LOCUS       DAOTEI010000001        52154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_484_length_52154_cov_5.996161, whole genome shotgun sequence.
ACCESSION   DAOTEI010000001 DAOTEI010000000
VERSION     DAOTEI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 52154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52154
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_484_length_52154_cov_5.996161"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="P5313_00005"
     CDS             complement(<1..156)
                     /locus_tag="P5313_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRY78770.1"
                     /translation="MDIPRIFTIAESAHRIHNPFTPEKLAVLGAALRLEPGARVLDLG
                     SGSGEMLC"
     gene            complement(262..945)
                     /locus_tag="P5313_00010"
     CDS             complement(262..945)
                     /locus_tag="P5313_00010"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRY78771.1"
                     /translation="MPDKTHSHLLVRVLVKAAFVIAVMAALFFGTAGSFRYMNGWIYL
                     GTITITLGTGITLLYRNDKALLEKRINTKEKETKQRFIVIISAILMTAIYAVPGFDFR
                     FGWSGMPVVLVVAGEIVLIVGYYLNLNVMLINSYASRVVEIQDNQKLIDTGLYAIVRH
                     PMYMAISLVYIGTCLVLGSYFALIPCMLMILVLGYRAVNEEKTLMNGLDGYSEYMKKT
                     RYRIIPYIW"
     gene            complement(1052..1597)
                     /locus_tag="P5313_00015"
     CDS             complement(1052..1597)
                     /locus_tag="P5313_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRY78772.1"
                     /translation="MNIEDKDELLTALSERFRKNPGRHRGIEWAEVEARLHGRSEAFK
                     TLGEMERTGGEPDVIGRDDKSGEYLFCDCSPETPAGRRSLCYDRQALDARKEHKPAGS
                     AVEAAEAMGVDLLTEAGYRALQELGEFDTKTSSWIRTPADVRALGGALFCDRRYGRVF
                     VYHNGADSYYSVRGFRGMLRV"
     gene            1836..2672
                     /locus_tag="P5313_00020"
     CDS             1836..2672
                     /locus_tag="P5313_00020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY78773.1"
                     /translation="MDFQGKRALITGASMGLGKALAEECARRGMDLFLVALPDSGLPV
                     LAGELAGEFGVETAWLEGDLTEASTLERLRDRVEEEGLDIDLLINNAGIGTPGHFLDH
                     SLEYHEATIELNALALVRLVRLFLPDLRRRNASILNVASLGAFFPMPTMPIYAATKSF
                     VLHFSLALREELGGTVGVSVLCPNAIRTTPDVNRYIDNLGPLARRACFTPECIARSTL
                     DGVARNRAVIVPGAVNRVIAFISQVVPRALINRTIRHYWGTFLRCGCGKLAPAGGGAD
                     HV"
     gene            2665..3585
                     /locus_tag="P5313_00025"
     CDS             2665..3585
                     /locus_tag="P5313_00025"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRY78774.1"
                     /translation="MFDPAVPGVGGRAPVAILYFSGVGGTEVAARLLGRLAGPDTPCE
                     VLSVEDPRAARRAAEPGLLVLCYPTYFLKPAPVMLDFLRGIPRGRGDRQAVLVTTYEL
                     YTGDSNRRAALILRDRGIPVVGSYALRAPGTDVTCVVPDRLCGWLYRFERAFPRKAAK
                     IARRIAEFSAAPPTAGRIPAPKWYTPLAWVLQVLALDGFIRMRDSFAVLTDRCDSCGL
                     CVRDCPHGAWRKTQGGPEHSGELCDLCTRCIHRCPRKALVLVRRLRDNRRLDPALYGA
                     LGREAFEACAGFGKDGRRAAGLVPETGGSP"
     gene            3582..4283
                     /locus_tag="P5313_00030"
     CDS             3582..4283
                     /locus_tag="P5313_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY78775.1"
                     /translation="MNPRAAPKRMGRPVRRPGEPSTRDRILGAAIRLFSENGYEGTSI
                     KEITRAVNLTESAFYRHFKGKDELLAEIIAAVEGMVHQPLPGPEGQPAGGASVFRHLF
                     EIPADAFGNNPVALHVCRFFFMECPRNERIRAYLKRAMEQLADLEVGRILEEEIEAGR
                     ILPCDVREVSRLVNVLRYQWCYQVAILDWDEGYDPEKSKRDLDPVIRFFEGLYSPGEQ
                     KTRQDVSRALSQREE"
     gene            5183..5803
                     /locus_tag="P5313_00035"
     CDS             5183..5803
                     /locus_tag="P5313_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY78776.1"
                     /translation="MDHATFEENLERNPLRMWVRERYEVKPLRDAAPPGRIGRALHYC
                     CRNGTGTALLHRYFSPGRTAAVDPDAAMVDRARERVAGLPVDLSVGSLTRLAFADASF
                     DAVFVLGELHNYPEWRDCVAEISRVTRPGGLFLIEELSVESFEYGLGRYFRDRTEHDY
                     GSMLRLDEFVDLVRSSGFEILRLRKPVPFGLFRYFVLAARKTGAAR"
     gene            5846..7054
                     /locus_tag="P5313_00040"
     CDS             5846..7054
                     /locus_tag="P5313_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY78777.1"
                     /translation="MRGLEAEFLLDPGIRFLNHGSFGACPRRVFASYRRWQLRLERQP
                     VLFLDREHEGLLRRARAALGAYLNADPDDLAFVPNATHGVNLAAAGLDLGPGDEVLAT
                     DHEYGACDFAWQDLCGRRGAVYLRRPIGPPSRSRAELAEALWAGVTPRTRAIFLSHIS
                     SPTALRLPVEEICRRARASGIVTVIDGAHAPGQIPLDLTGLGADFYTGNCHKWMMAPK
                     GSAFLHVRRELQDRVRALVVSWGYRKPPGTGSGSRFLDLLQWSGTYDPAAYLAVPDAI
                     RFQARHGWDSVRSECHDLLRRTLERIADLSGLEPAYPPDSDAFAQMAVTPLPESLDLA
                     EAKARLYDEYRVEVPLILWGDRKFVRVSIQGYNTEADADALLAGLRGVLRTSRVSGSS
                     PARGPGSARS"
     gene            complement(7115..7897)
                     /locus_tag="P5313_00045"
     CDS             complement(7115..7897)
                     /locus_tag="P5313_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY78778.1"
                     /translation="MMRRIYAGRPHTVVLRALYALKTSAWFVVVSGFFEPVFYLLAFG
                     LGLGTLVGEVTGPLGRPVSYAAFLAPALLATSAMNGAIYDSTWNVFFKMRFARLYEGM
                     IATSLGTLDIALGEILYALLRGLVYALAFLAVMVALGLVPSAWGILAVPASLLIAFGF
                     SACGMALTSYMTTFQQMDWINFFMLPMFLFSATFYPITVYPEYIRWIIQAFPLWHGIE
                     LIRGLTLGQFDGSILRHVLYFAAMIAVGLAATTRRLTNLFMK"
     gene            complement(7894..8745)
                     /locus_tag="P5313_00050"
     CDS             complement(7894..8745)
                     /locus_tag="P5313_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY78779.1"
                     /translation="MSDAISVHAGASAYFGRRRSARSMAYGTWYVTEHRLRTMRAYLG
                     SIIAGSVFNPVLYLFALGVGIGSYIDSNAGAPGIGGVGYLVFVGPALLVSAALTVAFE
                     ECSYPVMGGFKWTREFWAMNATPIDARQVVNGVLLAAGFRIVFTVAVFWCCLAAFGAS
                     PSGYGFLAMPAAVLSGLAFGSVIMAVAANLEEDDGWFPIINRLIITPMFLFSGTFFPL
                     EGLPIWLRWLGWISPLWHGTQLGRAAAYGMSEPVWLTLVHVAYIAGMLGVGLGLSWRS
                     FRRRLSK"
     gene            complement(8742..9656)
                     /locus_tag="P5313_00055"
     CDS             complement(8742..9656)
                     /locus_tag="P5313_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY78780.1"
                     /translation="MIKARGLTKDFGEVRAVDSVDFEVRKGEAFGLLGPNGAGKSTTM
                     RMIASVSRRTGGELSILGLDPNEHGPDIRGHLGVVPQQDNLDVEIRVRDNLVVYGRYF
                     GLPMKFLGPKADELIEFVQLTEKAKAKVDTLSGGMKRRLTIARALINEPDILLLDEPT
                     TGLDPQARHILWDRLFRLKEAGVTLVITTHYMDEAEQLCDRLIVMDKGKIMAEGTPAR
                     LIREYSSREVVELRFGSDRNAVLAGQLAGFGDRIEVLPDRILIYTDTGEEDLARIVER
                     GLRPLTSLVRRSSLEDVFLRLTGRSLIE"
     gene            complement(9739..10197)
                     /locus_tag="P5313_00060"
     CDS             complement(9739..10197)
                     /locus_tag="P5313_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRY78781.1"
                     /translation="MTVEEYLHSLPSDQKEPYQKLRQTILEKLPEGLEERIQYGMIGY
                     VVPHSLYPAGYHCNPKDPLPFLALAAQKGSINFYHMGMYARPDLLEWFKIEYSKVSRH
                     KVDMGKSCVRFKYYDEIPHDLIARLLGKMSVRDWIGLYEAGRGKGGRTDS"
     gene            complement(10255..12033)
                     /locus_tag="P5313_00065"
     CDS             complement(10255..12033)
                     /locus_tag="P5313_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRY78782.1"
                     /translation="MIRRAAIPALLALLLPYHAGAQGSPKEVLVLHSYQNDYEWTRLI
                     NQGINEELEEARDLSCQVKSEFLDAKRAWDEGRAEAFAGYLSRRYSGFPPDLVLVSDN
                     DAFEFMLEHGDGIFGPVPWIFIGVNDFSATSIARVRNRVTGVVERIDYESTLRLILEL
                     FPETREVKVLSDRTTTGPKTLHELLMTAESMHFPLPITSFPDVPFREVLRTAVGLPDT
                     TAVFLLAYARDGAGEVLSARDVASRVGAAASGPVFAIWDFFLGHGIVGGALTSGLDQG
                     REAGRMALRILRGESPGAIPVSDVPATPVVLDWRQLRRFRIPAGRFPPDVRFEYRPLS
                     LRERDPAAFWITVGLGTVAVVLAGSLAVIALAYGKVRQSEDRLAASLREKETLLREVH
                     HRVKNNFQVTASLLSLQAGTVEDERARAALRDSENRIRSMALVHAEVYEERNLADIDL
                     ADYARSLAGSLISSYAESAGASRFAVSGEPLSLGMDRAIPLGLALNELITNSLKYAAR
                     GRGGCAMELEIRPDGESGWSLKYSDDGPGLPADLSLENPKTLGLQLVTLLAQQAGGRI
                     DRNPERPAGYVIRLGAVPERPGPYGN"
     gene            complement(12079..13098)
                     /gene="pdxA"
                     /locus_tag="P5313_00070"
     CDS             complement(12079..13098)
                     /gene="pdxA"
                     /locus_tag="P5313_00070"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRY78783.1"
                     /translation="MGDAAGIGPEICAKTLARPGLWETCRPLMIGDRASMAAALESLP
                     AAGRPSFVPVSPREMEGLGPGTPEFGGIPLYQPGSPLSGVVPGKLSAEAGRGAVEFVK
                     AAVDLAKAGRVAGIVTAPLNKAAMHAAGFSYPGHTELLAEGFGVKNYSLVLSARGTFV
                     FHATTHVSLRRALDLIDENRVYAQIRLAHLLARALGRGGETVAVAGLNPHAGEGGLFG
                     TEEIDSILPAVERARAEGIPVEGPLPADVLFPRAARGIYRFLVAMYHDQGHGVFKTLY
                     FDEGINVTVGLPVIRTSVDHGTAFDIAGKGMAKDESLAEAVRLAASLGPVWAEIREAA
                     RAALE"
     gene            complement(13125..>14304)
                     /locus_tag="P5313_00075"
     CDS             complement(13125..>14304)
                     /locus_tag="P5313_00075"
                     /inference="COORDINATES: protein motif:HMM:NF028352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRY78784.1"
                     /translation="AYAAVRKAAGVLAAAGYLPVYKKIDSTLRGPAGRELDAVMDASG
                     ADLAVLCPAFPANKRIVRDGILRVDGVPVAETALSRDPVNPVKESRIAAYIVGQSRRP
                     VRELGRSVLDGGEAAVRSALEDFRRAGGGIAVCDAGGEADLALLARAALSLGSGVILA
                     GSAGLALPAAEILADRAGRGRPGGAAAGSAGSVSVRPDARTRPVVLAVGSVNPAARVQ
                     ARTLIEAGAREFRLDPAEILEARSPAEAEAAAEALGLRAREALQTGGKVLIATPGDRS
                     DVEAAQALGAGRGLSGAEVAAALARALAGAVRAAIRDVPDPGVAATGGDVARALLSAL
                     GSRAVDLLGEVSPGIPLCAVRGGERPGLFLVTKAGGFGAPDALEKAAALLSTCTRAEP
                     "
     assembly_gap    14305..14314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14322..14510)
                     /locus_tag="P5313_00080"
     CDS             complement(14322..14510)
                     /locus_tag="P5313_00080"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRY78785.1"
                     /translation="MKYAVIADDLTGAGDTGAQFARAGLRTRSLFGDWAPEDIEGSDA
                     LVVNTDSRPLDPAACARE"
     gene            complement(14582..15199)
                     /locus_tag="P5313_00085"
     CDS             complement(14582..15199)
                     /locus_tag="P5313_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRY78786.1"
                     /translation="MRNRIFPFDRIERPGFPAAAAAVLTLSLLNLGFSWTNNVVLKSP
                     LFLDSIFTAVGGAVLGPWAGLGIGLATNLGMEPIYGMTGYYWPFAACNMATGLIVGLM
                     SRRGAFSQGWHATTAVVLVTLANALLGGFIANLVFSGDTGVQIDNIVSAFTETGLSLF
                     TANFWARIPTNLIDKMLAVYAAFWVRAWLRGRVSGPGAEGGPVQV"
     gene            15225..15785
                     /locus_tag="P5313_00090"
     CDS             15225..15785
                     /locus_tag="P5313_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78787.1"
                     /translation="MIRKFSPYLSTYSNGILIILILFSFLGMTALISRSRRETTGKLL
                     AMDELLAMEAPAPSDWRSVPDALSGSHPGPAGRELDRLLGVYRTAVALLPDSRSDPGY
                     AEAEIRVRRLLYRVSKSYTELLRQREASFDSLFYFLAVGILVQSFAFLLSLYQVRVAR
                     EELVRQDQLFNLVQKVREEERRGLAS"
     gene            15897..16373
                     /locus_tag="P5313_00095"
     CDS             15897..16373
                     /locus_tag="P5313_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY78788.1"
                     /translation="MTYGLAPLHLDQAGLAASVRELAAEHLRRGGPPVDIEELGWDES
                     RLDPDTRLVLYRAVQEGLANIRKYAGAGKVELRLVGSHPYLILSLRDDGRGFDPRSRG
                     GPTSPGGGGLGLALLERQVRSLGGEYSLESRPGAGTRLQIRCEPGKERPWTPSAST"
     gene            16349..16990
                     /locus_tag="P5313_00100"
     CDS             16349..16990
                     /locus_tag="P5313_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY78789.1"
                     /translation="MDPIRVHVIDDHALFRRGILEILSKWPEVRSAGESETVDTFLDS
                     PMNQAVDLLLLDLSMGEGLNLDRLPEIRARLPELRVIALTMHNKPVLVKKTVNAGVQG
                     YVVKQSPPEVLRAAVRRVSEGGRYLDPELSESLYQLILDPSRDDASGYAALTPREQEI
                     FRLLAEGLSAELISRKLFISKKTAENHRFRIMQKLGLTSIAELVSYADELGVI"
     gene            complement(17224..18411)
                     /locus_tag="P5313_00105"
     CDS             complement(17224..18411)
                     /locus_tag="P5313_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY78790.1"
                     /translation="METRVYGYRWVVLGSFMLVNLTVQLLWISYAPVTGVAAAFYGVS
                     DLKIGLLSMVFMIAFIPLSIPVSWSIDTLGFHRTVGFGSLMMGAFGILRGLAGANYGL
                     ALAGSIGIAAAQPFLLNAWTKVPALWFPPKERATAVGLVTLANLTGTALGMVLTPALV
                     EASGIPRVQSYYGTVSAACALVFVLAARERPPLPPSEDAGAERALMLDGLRHAFTVPS
                     FRRFLAVAFIGLGIFNGVTTWIEAIVRPRGFGPVQAGTLGAVLLVAGVAGAVVLPALS
                     DRTGRRRPFILLGTTLAIPGLAGLAWTRSFAGLCLAAASLGFFLTGVLPTGMQYASEA
                     ALPTPEGTSNGLVQLFGQASVVFVFLMEALRTRDGSFTVSLAAACALLALAAVLAATL
                     KES"
     gene            complement(18486..19412)
                     /locus_tag="P5313_00110"
     CDS             complement(18486..19412)
                     /locus_tag="P5313_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRY78791.1"
                     /translation="MTRPKRSGKRLFQILLFTLTCPLFTLSSCSFEITATRYALVYGA
                     QNYDNATDLNYTDDDAIFMKAALEAAGWSVIVIREPKVTRDQFLQDIMDLANNPDVQS
                     DSSILVYFSGHGTFDSDGNTYICPTDTTFDGEGETEYSSCISSGEILSALQAFSCRNK
                     VAILDTCYSGGLVSVTNTGTTDAADPDYTNGSSTSTLLATLKNYSTLFGDAFRTGSVS
                     DPIVVTAAGSQEESLELSTYGHGVFTYFFLQAAEKGDLNGDGHVTIQEAFSYAKGKVE
                     TNFNTLGEIYPTYSGSEFLPRISGNARDIVIF"
     gene            complement(19409..20107)
                     /locus_tag="P5313_00115"
     CDS             complement(19409..20107)
                     /locus_tag="P5313_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78792.1"
                     /translation="MTRSRRSRTGVEAARLHPPVALLAVFVCLASGAAAQTASARPAG
                     VLSDDGGIAAGRPAWSIDVLAAPMLVLSGDGLAPVLPHGGFTFGTGTELRAGAWIPLR
                     IQLEYFHVFPSAISSAGDLYRSFAGWDLALGAGARVLSREGGAPLGLDLLGGVSFGAA
                     RYPGTTVAFATFSADLTVRADFTKSGNGGWRAVLPLSWIFRPGARSFSAGLGAGYVLP
                     PGRRSGAAVPGASE"
     gene            complement(20172..21440)
                     /locus_tag="P5313_00120"
     CDS             complement(20172..21440)
                     /locus_tag="P5313_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY78793.1"
                     /translation="MHLYERIYLDLRRLVEEGEVRPGERLPSIREAARKYGCNKLTAQ
                     KAYDLLSAAGLAENRVGSGSFALPPGSGQAREGELAVSVLSEEFFPYGPAGDLLAEAL
                     KSERGRVFSAPPVRGVPGLRESLAGLYRLPEESLLVLSGAQQGLELCRRLFGDRARPV
                     VLAEEPTYPAALALFRPSGHLPLGSEGPNPEEFDRFWMSAGPGPRVFYTVPDLHNPTG
                     LRYSRERKRALADLARRRDVWILEDDYLSETDPSSTPRFADLVPERTIWIKSLSKTTA
                     PGIRVGLLSAPDSVLTRLENLKSESDPGPATWLQLFLDRFYRSGLYARHLAACASAAS
                     ARRAELDALLSRFPGLSRDPASAGWNLWVTADREAAPASPPWAEGRHFGSNPATRRSF
                     RISFMSPGPSAWAGALGRLESALASAYPKV"
     gene            complement(21448..22359)
                     /locus_tag="P5313_00125"
     CDS             complement(21448..22359)
                     /locus_tag="P5313_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRY78794.1"
                     /translation="MKTGLSTAIFLVKIMIPVSLAVALLDWSGLLYHMARFLAPAMRL
                     IGLPGEAALALVSGFLLTNYSAIAVVTVLGLPVREASIVAIVCLTAHNLIVETAVMRK
                     AGSSALKMVLVRLGMGLAAGWAFNLLLPGSPAPASFAPPPQAVAVWSTLPPALAAWGL
                     GTARLVLKIFVLVVAILVSQRLLEEFHTAEYLARLAAPFMRGFGLPASASLLWLVINL
                     VGYAYGAAVIMEQIEGGKMKRQEADLFNHHAAVSHSLLEDTILYAALGIPVFWLTVPR
                     LAMAILVVWAERFRRTAFRRSFRVGTV"
     gene            complement(22451..23215)
                     /gene="rsmA"
                     /locus_tag="P5313_00130"
     CDS             complement(22451..23215)
                     /gene="rsmA"
                     /locus_tag="P5313_00130"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRY78795.1"
                     /translation="MNPDVRRRIADLAEAEPESLVWEIGPGLGAMTAELLDRDFRVEA
                     FEIDPGFSAALREIYGELPGFRLREGDFLRTRAAALAEARPDRVLGNLPYNAAAAMLA
                     ALLEGGRPPARMVFTVQKEAARRMASGPGTKDYASFTVLCRSVCEVRIAFDIGSSSFW
                     PAPRVTSSVVLLSPRADAAPEAGTPGFTRFVRSLFSSRRKTAVNNLRALGRSGPDAAE
                     ILRGMGFPADARAEALTPDELLAFYRALGGPDAGRD"
     gene            complement(23115..24884)
                     /locus_tag="P5313_00135"
     CDS             complement(23115..24884)
                     /locus_tag="P5313_00135"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY78796.1"
                     /translation="MTNHPDSSLRDCALVFASCLFLAPPGRAPAGFLPAALALWGAAR
                     ALEQGPGRGAGLSGVVLAASAPPRGLTRASGPHPVPGPALGPPRSRGRGGLPYRLRAA
                     AAALVLAFLARRSALEAGAPASFGLNAGVVEWVEGTAAEDSSPLRTGGRRLALDLRAA
                     GSGSDVRASAGGRLTVILDRPCAVVPAGARIRVRGRLVPDREGSGWVLFARGKDVEDR
                     GYASGWVDSRAVIRAGLLDALSRAGGEGGALLEALVLGVRDDLDLDLAEAFRKAGCAH
                     ILALSGQHLGILAALVSFLAAPLLGRRGALAAAMLLAGAFTVFVGPKPSLVRAALMFA
                     YSGIARLAGRPQGAANGLAAAFLVQCLAWPGVERDLSFQLSYLALAGLALLSPAFEYL
                     ILPRVPPVPAKALAASLAAQAAAGPRTLAVFGTLYPAGIPAAALAAPLTAAFIWAGLL
                     GAGACAALPILEAPVRAVLGLLYGALRLTMEAFAWVPGPTVPEGAGRGAWACVVAAAA
                     LAVYAVPHVDLYRTRRRLPAELRFPEGPGGLPGSPGPGDAQALRPELPGEPRRAPPDR
                     RPGRGRAGVLGLGDRAGPGGHDR"
     gene            complement(24993..25637)
                     /locus_tag="P5313_00140"
     CDS             complement(24993..25637)
                     /locus_tag="P5313_00140"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRY78797.1"
                     /translation="MPPAEPSPPGSFPGKAEARAWARALLRGVSPGDRIVLSLAAADR
                     ILYMEAFRESSLVLAFLSMPSEIDTSAVIRAALRAGKAVAAPRIEGGEIAFAYLDESW
                     ESLPRDSLGIPTPPAGEPIALEDLRRRKVFALIPGLLFDRTGGRLGRGKGYYDRFLAS
                     VLATLGTGTAGESGFFVPCGFCYETQVVPLVPVTERDVRVPLVATESGLFRAGR"
     gene            complement(25624..26142)
                     /locus_tag="P5313_00145"
     CDS             complement(25624..26142)
                     /locus_tag="P5313_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HRY78798.1"
                     /translation="MEKYWFTYEDIHSTIRDLAERIRQSGYDADAIVAIGSGGFIPAR
                     ILRTYIKKPIFAVGIAYYDDENKPTDTPHKVQWVDEVERKLTGKRILLVDEVDDSRTT
                     LEYCIRELLTHKPASVAVAVLHNKDKEKRGVIPPEVKLYLAGRELEDRWICYPWDALD
                     IEEQNRQAHAAR"
     gene            26476..27237
                     /locus_tag="P5313_00150"
     CDS             26476..27237
                     /locus_tag="P5313_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRY78799.1"
                     /translation="MAYTVSELAELAGVSVRALHHYDRTGLLVPGRRSRSGYRLYGDE
                     ELRRLQQILLFRELDFPLGEIRRILDRPGYDPAEALEAHRGILREKGERIARLLDLLD
                     HTIAQMRGETTMLTDKELYEGFSEERIEAMKEEARRRWGRDRVDESHRRVAALGKEEF
                     ARIKAAGEALEARFAAAFRAGEAPGSERTREICRGWEAHIGNFYEPGPDILEGLGRLY
                     AEPGPFRDRYEALAPGLADYLKESLALYAASRRKD"
     gene            27330..27833
                     /locus_tag="P5313_00155"
     CDS             27330..27833
                     /locus_tag="P5313_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRY78800.1"
                     /translation="METTLYEFKARAADGKDFDFEALRGRPVLIVNTASKCGFTPQYA
                     GLEALHQEFKDRGLAVIAAPCDQFAHQEPGTDEEVVGFCRRNFGVTFPILSKLEVNGP
                     GAHPLFVWLRGKTRGLLGDRIRWNFTKFLVSRDGTTVRRYSPATAPEALELDILSALE
                     AQQSSYH"
     gene            complement(27830..28249)
                     /gene="arfB"
                     /locus_tag="P5313_00160"
     CDS             complement(27830..28249)
                     /gene="arfB"
                     /locus_tag="P5313_00160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRY78801.1"
                     /translation="MNLGQLRASLERAARLDFARSSGPGGQNVNKTSTKAIARVSVAE
                     LEGLSDAERERVRERLDGRLTAEGDLVVYAQDERSQVLNRAAALARLESLIVKAAHRP
                     KERRPTKPTRASRERRLSGKKARSEVKRGRGRPEGET"
     gene            28345..29271
                     /locus_tag="P5313_00165"
     CDS             28345..29271
                     /locus_tag="P5313_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78802.1"
                     /translation="MKVRNNLAQITKKSLCEAINLDMMSRLARDVFSEYDLHERTGFP
                     STVPITSQLAAHHIVNDAIAENRFLHLAERLILLDREGYMGRRYPVPGLAEIVKQIRV
                     EGYVWDEEAELFMEDPRVRRTPNWGRLLDDDEFRFALLRLDVANNSKMVQTHGDSAMR
                     GALEDLRQIVSRCVEFRAGRIWQWEGDGALAGFLFGSRVMSAVLAGMAVLHELFLYDR
                     LHNRLGEPLRIRAAVHTGSFRYHSDPEMLVKQEIVQETEEIESRLTPVGSLVITPAVA
                     PSLDRVILERFRPVQHFAESRLLAYEVRMEGT"
     gene            29268..30230
                     /locus_tag="P5313_00170"
     CDS             29268..30230
                     /locus_tag="P5313_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78803.1"
                     /translation="MRILVFSETPDRVEGALKAALPKDADLEILPLRGLKRTVQKPLN
                     ADLVYLDIADIGEAGLGRYASALASAEKLPWGVLDTAGELTDPARAFFRGARDYLGPG
                     ALRAGITPARLSEIRAFAGLECPNPAAPEPAKRFPGWDRLPEGSEIDVRFCYVSVGDP
                     EGLRERIGEKRLRKLREDFAGFMASWAAECGGIAWMRDPEGTLLLFPERDEGTSPVLA
                     AFRLILDRALVGYEVFRLEVPLTFRFSFHAGRTVWRRPGSTGTIVSGDVNFIFHLGGK
                     YCSDGRITLSAETEASIPAPIRDLFVPAGDFEGRRILASRKFRD"
     gene            complement(30440..31231)
                     /locus_tag="P5313_00175"
     CDS             complement(30440..31231)
                     /locus_tag="P5313_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY78804.1"
                     /translation="MIRLESVEVVFGRGGPDERRVLDSLTLAVPRGQHVSVIGSNGAG
                     KTTLLNAIAGSVPLESGRILIDGADQSRFPEWKRARYVGRVRQDPLAGTAGDMTVLDN
                     LALASRKGPRRLRIAVPSRLRRELRDRLAELGMGLEDRLRENVNRLSGGQRQALTLLM
                     ASISRPAVLLLDEHTAALDPRNAELVSGLTRRFVGEYGLTALAVTHDMRRAIDEGDRL
                     IMMHEGTVVADVAGDVKKGLTVERLVDLFRRTRGGDYAEDRDLLS"
     gene            complement(31228..32124)
                     /locus_tag="P5313_00180"
     CDS             complement(31228..32124)
                     /locus_tag="P5313_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY78805.1"
                     /translation="MIEGVLVEGLVYGILALGVFISFRVLDFPDLTVEGSFPFGAAVG
                     SALIVARVPAPLALGAGFLAGTLAGAVTSGVYHGLRVPPLLAGIVTMTGLYSVDLRVL
                     GGRANLPLIAYNPVMRAAESWLGGSFDPSIQAFLAFLLIAGALVLVLDLFFHTEAGIA
                     LGALGDNEASVVSAGVDPARLRTVGICLANGLAGLSGAFAASYQGFADANFGAGIVAA
                     GLASVMIGEFLFRTNLIGGQLARVLLGSVLFRGIMYAGRSWGYLAGITPNDLRLLTAV
                     LIVASIAASRYKGRTPAGRRRA"
     gene            complement(32128..33111)
                     /locus_tag="P5313_00185"
     CDS             complement(32128..33111)
                     /locus_tag="P5313_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY78806.1"
                     /translation="MKRIAVSALALAALGVLLSSCSPKIAPVVGVAKFVSHPALDALE
                     KGITDEISAARKDVQFDLQNANTDMGTAAQIAQRFKSSKVKVAVGIATPTAQAMANAI
                     KDIPVIYSAVTDPVSAGLVASLDKGGPNVTGTSDMTPVREQLDLLLSLKPKARVGHLY
                     NAGEANSVKLAELVQAYCAEKGLSYVPATVTNSSEVRQALLSIAGRVDGIYLGNDNTV
                     FSAISAVTEICNERKLPLVTADPSSAETLPILAAMGFDYYAMGRSTGKIVLRVLAGEK
                     TADIPAYFAKDPSEMALVLNKDVAAAIGVSIPADLEARASLVIEGGAARRK"
     gene            33284..33628
                     /locus_tag="P5313_00190"
     CDS             33284..33628
                     /locus_tag="P5313_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HRY78807.1"
                     /translation="MKRAEDIDLIARNLKEMSVTLAAAHDPRLIEEFLYSLFTKSEAD
                     EIAKRWALVKDLASGIPQRQIAQNLGLSLCKITRGSRELKKPESAFRRMLQIAGIRIE
                     DRKKTRGTAKAG"
     gene            complement(33804..36836)
                     /locus_tag="P5313_00195"
     CDS             complement(33804..36836)
                     /locus_tag="P5313_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY78808.1"
                     /translation="MRPRRLVLANFGPYRGTAEVDFDRLGDVFLVCGKTGSGKSSIFD
                     ALTYALYGRAPESRGSLERELRSHLALPEEETRVELEFWLGLDAFRVVRTPPFRRPPK
                     KATKAGSAVEEPAAAALYEGHGEGARLVSDRISEVDEILRRRIGLTQEEFTKIVLLPQ
                     GAFQRFLEMNSTDRTSVLQKLFPVDLHDRTAALAKDRAADVRRRLEYLDGELARLDAE
                     LGGPGEGGSAGALLSDLEKALADARDRERAAQAELETRKAAFREARETEALLERRDRE
                     ARALAAREAGRPAREAWGRDLDRDSAARSAAPRVDAREKAEADLETAREELRKTRAEV
                     EALEAREGEAAESARKAEDLAARAAALDAEAGALAAARAAWERARAAAAAGEAARAAE
                     ESARAEAEDRARELDEAERGYRGLALSPEEEEGARERRETARTAREEARKLRDRASRG
                     AELARKRDAAREEAARKDREAEEAVRDAEVAEARLSDLARERDLSAAGVLAAGLEPGE
                     PCPVCGSRDHPAPAAPRPEAFGLEDRLREAGSSRDAARAREALARGRARDRSREAEEA
                     EAELAALGNVPEEEAARELLAAAEAEESAAVEALRALAERARAAAAARSVAEALAEGL
                     RSARERLSAASRSAAAAGAAESEARASAGDEDPTERLELVRRERAEADRARKAAEEEV
                     RNHRDGLSSARARRAVAEERIPALEAALETAREAEGSALAAAGFRDGAECRTALLSGP
                     DRARLASEAERYDRELSEARARARAAGEAAEGREPGDPEALAREAEAAEARLEEARAE
                     VLASSGRAEAARRGESRRRVLSEERRTLEERGRTLDGLSRLLNGELSGSRLPFKNYAL
                     SLYLRQVARAASRRLLDMSDRRYSLSVDEGPARGTAKAGLDLSVHDSFTGRSRPAGTL
                     SGGEKFLASIALALGLADVITRRAGGIVLDAVFIDEGFGSLDDEALDRAIEVLDRVRE
                     GRMVGLISHVPELRDRIPARIEVAKGRGGSRIRIEAVD"
     gene            complement(36823..37971)
                     /locus_tag="P5313_00200"
     CDS             complement(36823..37971)
                     /locus_tag="P5313_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRY78809.1"
                     /translation="MKILHASDLHLGKTLHERDLVPDQAAMLDQLLAAAVEHDPAALV
                     IAGDVYDKAVPPPEAVSLLGNFLGRVKEARPDLGVLVIPGNHDSPARLGFGDSLFRRA
                     GVHVRTRPEDAAEPVVLERDGERLYLWALPFLGAGAFPDAPRSQSGRFRAALDLIVPR
                     LAGDGANVLVCHAFAAGGLASESERVFLGSAELVDADAFEPFDYVALGHLHRPQKAGP
                     KGRYPGSPLGYSFSEAGQEKGFLLVEVRRGGFEAVPVPVKPLRPLSRLEGSYADLLES
                     GAGRAEKDHYLEILLTDPEPVVSPVESLRRVFPNLLSLRQAAFENPSLGEAEAAEAPA
                     RASASRDGAGAALEDFLAFHRAVRGADPEPPVLELFRELLSEAADAAP"
     gene            complement(37968..39236)
                     /locus_tag="P5313_00205"
     CDS             complement(37968..39236)
                     /locus_tag="P5313_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HRY78810.1"
                     /translation="MCDTFWTPGAGLSYFGKNSDRKPAEPQAVCILPRAPEAPRSAVS
                     GGELPYPDKGYAVLLSKPSWIRGAEIGLNEKGVAIGNEAVFSKWPARKDGILGMDFLR
                     IALRSASTAQEAVEILVELTERFDQGGNGAYRGRLFYHNSYLAAGFDGAFVLETAGKR
                     WAWRKAEGPSAISNSYSIGLDYKRLDAQTRKAISPVNEKMACYDEADAGRIGEKESWK
                     PYVESRLHAFLTQGDSRRRAVEGRLAAAGPGLDLAVVLDTLRSRSLPDPARPGKTAVI
                     CAHDGDNLGYPTTASLAVEYDPRAGRCVAWFTGTSYPDISLYKPVLLSKGEFTPLWTG
                     YDYSEDAPASLEYWKRGRDLYRRGGKALSPDPEFRKARDSAQERLREAAGRVEADGIE
                     AVRKDVARIVSDWEASFDLSGRGAPRQPSA"
     gene            complement(39247..40974)
                     /locus_tag="P5313_00210"
     CDS             complement(39247..40974)
                     /locus_tag="P5313_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRY78811.1"
                     /translation="MKRSMRAGFVFFALLVLGTSLAFAAPPGTTGAALGEAKVFAAAS
                     ANAKVTATLSAGDSFRVLETGAREEAVAGRRGRWLRISTPDGVQGWLHGGWAVVSTSF
                     LKLADFPDSSAYGRYIAWAFPKGERVRAVQDYEKVAKGDLGWFFALDEEDPPILVVWD
                     KDLDATPWVEALPEGFPRILSGRVYFVYPDAVEPVGEKALADFPKLAEQWRTSASYET
                     LFPIGSRVVLGEHAALSKTDPDSKNWGDEMSDYVGLEAVVTGHQGQDPWGRPVVSVDV
                     DEGQFYWRTENMELLERGTGEVVDSGDEDYGDGDYSYEDYDDEDYALEDAFPVGSLVV
                     LGRHLQLDPEDEESAYWAPEMDEYVGMEAYITEHAGLCPWGEPTALVDVDGGTFYWRL
                     SDMELLEEGPGYWEGGEVYDDEGEGEYEDLGEDLSGMVGVGTKVILGRHDDRNGGDNW
                     APEMDAFVGKQATVTEIIGADSQGFLVVGVKENTWSWRIRNMAVVGRGVAGSYGFQVG
                     DRVVLGRHRFVDGNENWNPSMDAFVGMTARITELVGTKGDGSDCFLVRVDADKGKWVW
                     RAESLTPVK"
     gene            complement(41095..43599)
                     /locus_tag="P5313_00215"
     CDS             complement(41095..43599)
                     /locus_tag="P5313_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRY78812.1"
                     /translation="MELNQEYVDGLSVNEVAVMNRIAGELGIRTGQVSAVVSLTAEGC
                     TVPFISRYRKERTGSLDEVQVRDTVHKYESYVNLETRRLEIVRAIFGQGKLTADLHDN
                     LLKCSSLAELEDIYAPFKKKKKTRGMLAQERGLEPLAELMVSGDDAAVEARAPEFVRE
                     DPENPELSVPTARDALQGAMDILAERASQDPENRALVKSWYLKTGRLTVKGVGDESAK
                     AKSVYQMYWEYSEELSRVKPHRVLAVNRGEREGVLAVTLDVDEDGAAERLKARYAPRT
                     TYHRDALDDGLRRLISPAVLREIRSDASDAAEGHGIDVFSENLKNLLLQPPIKGTRVL
                     GVDPGIRTGTKCAALDETGKFLDYFVINQETKPEEGKKAVAEAVRRHSLALIAVGNGT
                     ASHEVQKLVADAISENGLSVAYTVVDEDGASVYSASDVAREEFPELDLTIRGAISIGR
                     RLQDPLAELVKIDPKSIGVGLYQHDVNQKRLSDTLDEVVGSVVNNVGVNLNTASHSLL
                     KYVSGINAGLAKKIVKFREANGRISGRDQLKGIPGLGDKTFEQCAGFLKIPESPNPLD
                     NTWVHPENYPLAEEILPVVREGRDLTKDLRAGLKEKYKVGDTTLDDILGELKKPNRDP
                     REDLPKPILQKGVLQFEDLSEGMTVTGKVKNVVDFGAFVDIGIKESALIHVSEVADRF
                     VRDPMEVLKVGDVKEFRIISLDPERRRIGLSLRKEGAARGGRGAGPSPERGAAGEHPA
                     GAGRVPEGAGTDRAAAAAPAGARRGAVVRKPGAQDRDGPARPERRDGFGGGPGSGRDR
                     NPYSQSARTPARGPKPEDDDGTTYNPFAELLKGRKG"
     gene            43843..44442
                     /locus_tag="P5313_00220"
     CDS             43843..44442
                     /locus_tag="P5313_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY78813.1"
                     /translation="MFSALEVANLCGVVNQTAINWIRNGHLKAFTTPGGQYRVYAEDL
                     RAFLEGRGMRIPDELSGKSRFDVDWNSLVIIDDDRELNDLLRRYFEKKLPSLAIAQAF
                     DGFEAGRILTEKRPGFVILDVDLPGVTGHSLCKRIKTDPSFGKPYVVAITGLDRPEER
                     SSMLEDGADAFFPKPLDFEEVTASLLEFAGKVRAPVAAG"
     gene            44429..44812
                     /locus_tag="P5313_00225"
     CDS             44429..44812
                     /locus_tag="P5313_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY78814.1"
                     /translation="MQPDKTRPALVLVVEDEDSIRLSLRDFLRGRGYEVLVASDGVGA
                     IKQLLDNDVDVIISDYRMETLGGDYWVRFLARYCPEKKIILTSGFLRPDFKVPFEVVY
                     KPFDYSELEALVGRLASEARDARRP"
     gene            44829..45080
                     /locus_tag="P5313_00230"
     CDS             44829..45080
                     /locus_tag="P5313_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRY78815.1"
                     /translation="MVSGRVQGVGFRWSARIQALRFHVSGWVRNLPDGDVETHAEGSP
                     GAVEEYLAWLEKGPPYARVESVRSRPVECRGYVSFDIED"
     gene            complement(45211..46449)
                     /gene="fabF"
                     /locus_tag="P5313_00235"
     CDS             complement(45211..46449)
                     /gene="fabF"
                     /locus_tag="P5313_00235"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009783150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRY78816.1"
                     /translation="MDRRRVVVTGLGVVSPVGNSVPEFWESIKSGRNGIGPITQINVE
                     DMSVKIAGEVKNFDPGLRMDAKEAKKMDRFSQYAVYAAREAWEDAGLGQGVQDPERLG
                     VCLGTGQGGTSSIEESAARLYERGPGRVPPLTVVKVLPNIGAANVAISVGATGPCQCI
                     VTACAAGTDAIGTGLRWIQEGHADVVFAGGSEACVTRLAVASFAMIQALSTRNDDPAH
                     ASRPFDKDRDGFVMAEGCGVLVLEDFERAKARGARIYCELAGFGTTCDAHHLTAPDPE
                     GRGAARAMTLALKDAGMRPEDIDYLNAHGTSTPLNDPIETKAIKLAFGDHARKLMISS
                     TKSMTGHCVGAAGGVEGVATVLAVRDQFAPPTINLENPDPECDLDYVPNRGRSAPIRA
                     AMKDSLGFGGQNAVVIFKRI"
     gene            complement(46730..47482)
                     /gene="fabG"
                     /locus_tag="P5313_00240"
     CDS             complement(46730..47482)
                     /gene="fabG"
                     /locus_tag="P5313_00240"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRY78817.1"
                     /translation="MADGLLKDSVAIVTGGSKGIGHAVVAAFLREGARVHYFSRTKAE
                     DHAELEALAASGGGTVAWTACDVADEKGVEAAVDAVAASEGRIDVVVNNAGITRDNLV
                     FRMSLDEWDTVVRTNLTSAFLVSRTAARHMIKKRSGSIINVSSVVGIMGNGGQTNYSA
                     SKAGLIGFTKSLAREVSARKVRVNAVAPGYIDTAMTEAIPEEAKAKLAAAIPLGRTGS
                     PEEVAKAVLFLASDLASYVTGEVLKVDGGLAM"
     gene            complement(47492..48412)
                     /gene="fabD"
                     /locus_tag="P5313_00245"
     CDS             complement(47492..48412)
                     /gene="fabD"
                     /locus_tag="P5313_00245"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRY78818.1"
                     /translation="MKACFLFPGQGAQYIGMGKDFYETSTAAKEIFDMASEISKLDLK
                     KLIFEGTEDDLKQTRVTQIALAVVEAAASSCIRDYGTKASGAAGFSLGEWPALVEAGF
                     LDLPVMFRLVAERGRLMAEAGERSGGSGMAAVLGLAPEAIEAALKGAGIDDVWPANLN
                     SSSQTVVSGTESGLGKIEVPLKAAGAKRVIRLKVSGAFHSPIMKYAYDGFKELLSDVE
                     FKDAKIPFYSNVTGGIITSGAEAKRCAAEQIISAVRWVEEEKAILAAGFDACLEAGPG
                     TVLGGLWKSVTETVPCHPCGTVDQVAAISL"
     gene            48834..49133
                     /locus_tag="P5313_00250"
     CDS             48834..49133
                     /locus_tag="P5313_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRY78819.1"
                     /translation="MSDIGDRIRAVLREIPAGRVVSYAGAAARAGVANGGRTTARILH
                     ACSRKDGLPWHRVIRSDGRIALPPGGGFELQKTLLEAEGVEVSPEGRVDMRRFGW"
     gene            complement(49141..50055)
                     /locus_tag="P5313_00255"
     CDS             complement(49141..50055)
                     /locus_tag="P5313_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HRY78820.1"
                     /translation="MFELDDKVVESVIFAMENQGAVSYIDMETGILSSRIDEGAEAGE
                     PGEDAPDLEPIPTWTSQDGFRLMEQFAQTIGHPVVRADLLAALSRGRGVFRAFKDALD
                     AHPEAERKWYEYKARAMKARIVQWYEDLRDVRGLQRLGLEPEDTDDLLLSDFRILRYG
                     AEVWGDFEPLVRAGREDALARFPGTLVDHVFSTLDREIRGPYGADYSIYSAECEPGAR
                     AGMALTRILRSGEGAFAVLGFLYVDPERRRIGLGRTLIERAREDAFREGIRHFVVDMP
                     FLWPEFGASLEGLGYASFGTRWLSSSGS"
     gene            50214..51101
                     /locus_tag="P5313_00260"
     CDS             50214..51101
                     /locus_tag="P5313_00260"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY78821.1"
                     /translation="MEPRGADIGFIGLGVMGMSMAGHLRKAGARLHVYTRTKEKVLAF
                     LEDGAEWHDSPGAVAAASRAVFTMVGYPQDVEEVYFGKAGILETARPGTLVVDTTTSR
                     PDLARKVWEEARKRDLHALDAPVSGGDLGARNATLSIMVGGSEPDFEEAKPLLLTMGK
                     TVVRQGPAGSGQYTKMVNQIVIAGALAGTVEALVYAEKAGLDPRTVLDSISTGAAASW
                     QLSNMVPRMLDGNFEPGFFTKHYLKDLRIALESARAMGADLPMLTLAESLFAKMVDRG
                     YAEKGTQALYLLYKNGIPR"
     gene            51112..>52154
                     /locus_tag="P5313_00265"
     CDS             51112..>52154
                     /locus_tag="P5313_00265"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HRY78822.1"
                     /translation="MYERPVLDPLPAIVSEGRFAYGTFAKPFGRTNMLDVERPYHHAV
                     PRFIKRFRLKEWVAFYLGSSRYILMTALYDAKSAALALFQGYDRETRKSFGFRRILPG
                     GFFRFGPSLDGCKVAYKSRRDLLAYDLDLSGGSLRVRVDHRRTRTCPALEGEFTLPWN
                     DRQTAMQTVCLPLGLNRAMYSTKALCPMGGALLIGGETADFSGPDSMGILDDHKGYYP
                     YRLRYDWVIGFGQDPKGRRVGFNLTDNPVKDPERNSENCMWVGGRTFPLPPVKVTRPR
                     GVYEDWVIQDTEGRVDLTFHPETRFDIRLNLLAAQADYSGPFGSFKGFVRSGEGEKID
                     ASWVYGIGEKKYL"
BASE COUNT         8532 a        18092 c        17546 g         7974 t
ORIGIN      
        1 gcacagcatc tccccggacc cgctgccgag gtcgaggacc cgggcccccg gttccaggcg
       61 cagggccgct ccgagcacgg cgagcttctc gggagtgaac gggttatgga tacggtgggc
      121 gctttcggcg atggtgaaga tccgggggat gtccaaattc atctccttgc ggtcgtgaac
      181 ggatacgggc gggacttcac ctctgcacat accgaagctc gcacgggtgc ggtctatacc
      241 ccacggcgat acggcaagat atcaccaaat atagggaatg attcgatacc ttgttttttt
      301 catgtactcc gaatatccat ccagcccatt catcagcgtc ttttcctcat tgaccgccct
      361 atatcccagg acaagtatca ttaacatgca cggtatcagc gcgaaatagg atcccaggac
      421 gagacaagtc cctatgtaca ccaaggagat cgccatgtac atggggtgcc gtacgatcgc
      481 atacaatccc gtatctatga gcttctggtt atcctggatc tcgacgaccc tcgaggcata
      541 gctgttgatc aacatcacgt tcagattcag gtaatatccg acgataagta cgatttcccc
      601 ggccacaacg agaacaacgg gcattccgga ccatccgaat cgaaagtcga atccgggcac
      661 cgcatatatc gcggtcatga gaatggccga gatgatgaca atgaatctct gcttcgtttc
      721 cttttctttt gtattgattc ttttttcaag caacgcctta tcgtttctat acagcaacgt
      781 tattccagtg cccagcgtga tcgtaatcgt tccgagatat atccacccgt tcatataccg
      841 gaagctcccc gcagttccga agaacaatgc ggccatcaca gcgatcacga atgcggcctt
      901 cacaagcact ctaaccaaaa gatgggaatg ggttttatcg ggcatgttct gtcctgcctc
      961 cacctgactc gtccggggcc tttatagaag tataggcccg cacccgggag gacgcaacgg
     1021 ccgcggatcc cggaaccggt tcccgggaac ctcagaccct gagcatcccg cggaagcccc
     1081 ggacggaata gtaggagtcc gcaccgttgt ggtagacgaa gacccggccg tagcggcggt
     1141 cgcagaacag ggcgcccccg agggcccgga cgtcggcggg ggtccggatc cagctggagg
     1201 tcttcgtgtc gaattccccg agctcctgca gggcccggta cccggcttcc gtgagcagat
     1261 cgacccccat cgcctccgcg gcttccacgg cgcttcccgc cggcttgtgt tccttccggg
     1321 catccagggc ctgccggtca tagcagaggc tccgacgccc cgcgggagtc tcgggggagc
     1381 agtcgcagaa aaggtactct cccgatttgt cgtcgcgtcc gatgacgtcg ggctcgcccc
     1441 cggtgcgctc catctccccc agggtcttga aggcctcgga tctaccgtgc aaccgggcct
     1501 ccacctccgc ccattcgata ccccggtggc ggcccgggtt cttccggaac cgctccgaga
     1561 gggcggtcag caattcgtcc ttgtcttcga tattcataag atcccccttg gcggccgtag
     1621 ggatggaaat cgagtccgct tccctgaaat atactggttt caagcagagt cggccgttct
     1681 atcgatccac gtgcggttga caataatcac gggcaacgat attatgtaag taaccgttca
     1741 cttacttaac ggaaacgtac gtcgatcggc tggaaaacgc gcgcgcgttg cggcgcggaa
     1801 gcgacggcgg cggtagggaa cggccggagg atcgtatgga ttttcaaggt aagcgggcgc
     1861 tgatcacggg ggcgagcatg gggctcggga aggccctggc ggaggaatgt gcccgtcgag
     1921 ggatggatct cttcctcgtg gccctgccgg acagcggact gcccgtcctg gccggggaac
     1981 tcgcggggga gttcggggtc gaaaccgcct ggctcgaggg cgacctgacc gaggcctcca
     2041 ccctggaacg gctccgagac cgggtcgagg aggaaggcct ggacatcgac cttctgatca
     2101 acaacgcggg aatcggcacc ccggggcact tcctggacca ctccctggag taccacgagg
     2161 ccaccatcga actgaacgcc ctggccctgg tccgcctggt ccgcctcttc ctgccggacc
     2221 tgaggcgccg gaatgcgtcc atcctgaacg tggccagcct gggagccttc ttcccgatgc
     2281 cgaccatgcc catctacgcg gccaccaaga gctttgtcct ccacttcagc ctggccctcc
     2341 gggaggagct gggcggcacc gtcggcgtca gcgtcctctg cccgaacgcc atccggacca
     2401 ccccggacgt caaccggtac atcgacaacc tgggtcccct cgcccgccgg gcctgcttca
     2461 cccccgagtg catcgcacgg tcgaccctgg acggggtcgc caggaaccgg gcggtcatcg
     2521 tccccggggc ggtcaaccgc gtcatcgcct tcatctccca ggtcgtcccc cgggccctga
     2581 tcaaccggac gatccggcac tactggggca ccttcctccg ctgcggctgc ggcaagctcg
     2641 cgcccgcggg cgggggagca gaccatgttt gatcccgccg tcccgggggt cggcgggcgg
     2701 gcccccgtcg ccatcctgta tttttccggc gtcggcggca ccgaggtggc cgcccgcctc
     2761 ctgggcaggc tggcgggtcc agatactccc tgcgaggtcc tgtccgtcga ggatccccgt
     2821 gctgcccggc gggccgccga gccgggactc ctggtcctct gctacccgac ctacttcctg
     2881 aagcccgctc cggtcatgct ggacttcctg cggggcatcc cgcggggccg gggcgaccgg
     2941 caggcggtac tcgtgaccac ctacgaactc tacaccggcg actcgaaccg ccgggccgcc
     3001 ctgattctcc gggaccgggg catccccgtc gtgggctcct acgccctccg tgcccccggg
     3061 accgacgtga cctgcgtagt cccggacagg ctctgcggtt ggctttaccg cttcgagcgg
     3121 gccttccccc gaaaggccgc gaagatcgcc cggcgcatag cggaattctc cgcggccccg
     3181 cccaccgccg gacggatccc cgccccgaag tggtacaccc ccctggcctg ggtcctccag
     3241 gtcctggcac tggacgggtt catccgaatg cgggatagct tcgcggtgtt gacggaccgg
     3301 tgcgattcct gcggcctctg cgtccgggac tgcccccatg gcgcgtggcg gaagacccag
     3361 gggggccccg agcattccgg ggaactctgc gacctctgca cccggtgcat tcatcgctgc
     3421 ccccggaaag ccctggtcct cgtccggagg ctccgggaca accggcggct cgaccccgcc
     3481 ctgtacggcg cgctcggccg ggaagccttc gaggcctgcg ccgggttcgg gaaggacggg
     3541 cgccgggccg ccggactcgt gccggagacg ggaggttcgc cgtgaaccct cgcgccgccc
     3601 cgaagcggat gggccgcccg gtccgcaggc ccggggagcc ctccacccgg gaccggatcc
     3661 tgggcgccgc gatccggctc ttctccgaga acgggtacga gggcacctcg atcaaggaga
     3721 tcacccgggc ggtgaacctt accgagagcg ccttctaccg ccatttcaag ggcaaggatg
     3781 aactcctggc ggaaatcatc gccgccgtcg aggggatggt acatcaaccc ctccccggcc
     3841 ccgagggaca gcccgccggc ggagcctccg tctttcgtca cctattcgag atccccgcgg
     3901 acgccttcgg gaataacccc gtagcccttc atgtgtgccg attcttcttc atggaatgcc
     3961 cccggaacga gcgcatccgc gcctacctga aacgggcgat ggaacaactt gccgacttgg
     4021 aggtcggccg gatcctggag gaagagatcg aggcgggcag gatcctgccc tgcgacgtcc
     4081 gggaagtctc gcgcctcgtc aacgtgctcc gctaccaatg gtgctaccag gtcgcgatcc
     4141 tggattggga cgaggggtac gacccggaga aaagcaagcg ggacctcgac ccggtgatcc
     4201 ggtttttcga gggactctac agccccggag aacaaaaaac gaggcaggat gtaagccgtg
     4261 ccttgtcaca aagggaggaa tgaaatgagc agtgtcctat attactatag cgccaccggt
     4321 aattgccttg aactttcccg gcggctcgcg gaggcgatcg gcggaacccg gttgtctccc
     4381 ctggcgaagt ttcggaacca aactctgaag gtggagcgct cctcccacgc aggattcgtg
     4441 ttcccggttt atgcctgggg cccgccgcgg agcgtggaag aatttttcgc ccggatagac
     4501 cttagcgacg tcggatacat ctacgcggtg gcatgctgcg ggggtacggc ctgcaacacc
     4561 ctccccatgg tcgaccggct gctccgctcc aagggccgcg gtctggacgc cgggtttatc
     4621 gtgcgcgccc cgggctatgt cgaagtagat ccccaggatc cccaggccgg cctcatagaa
     4681 tttgtacgtc ttcggtccgg ccccgtcgcg cgacccgtgg aagaacgcct gccggaaatt
     4741 gtccgggcgg ttcaggagag gaggaaatct cccccggagc gcggagcctg gttcggctcg
     4801 gcggtgggcg gctttctgca tcgcgcttcc ctcgggcaga tgcggaagct cgatagcagg
     4861 taccgagtat cggaagcgtg cgatggctgc gggatttgtg tcaggacctg tccgcgggaa
     4921 aacatccggc ttgaggggaa tcggcctctc tggaaccacg attgcgacgc ctgcggcgcc
     4981 tgcgcgacct ggtgtccgaa acaagcgatc acccaaaaca ccgccgtagc cgctgcgggc
     5041 cgacaccatg cgggggtgtc gctgggcgac atgcttctac gagcatgagt tcgcccgcga
     5101 aaagcccgga atccggccgc ggatcgccgt cccccccagc cggctgcaat taaccgggaa
     5161 aaccaaaatt taggagcaag tcatggatca cgccaccttc gaggaaaacc tggagcggaa
     5221 ccccctgcgc atgtgggtcc gcgagcgata cgaagtgaaa cccctccggg acgcggctcc
     5281 cccgggcagg atcggccggg ccctgcatta ctgctgccgc aacgggaccg ggaccgccct
     5341 cctgcacagg tacttttccc ccggacggac ggcggcggtg gatcccgacg cggcgatggt
     5401 ggaccgggcc cgggaacggg tcgcaggcct gcccgtcgac ctgtccgtcg gatccctgac
     5461 ccgcctggcc ttcgcggacg cgagctttga cgcggtcttt gtcctggggg aactgcacaa
     5521 ctaccccgag tggcgggact gcgtggcgga gatctcccgg gtcacccggc ccgggggcct
     5581 tttcctcatc gaggagctct ccgtggaatc cttcgaatac ggcctgggca ggtacttccg
     5641 ggaccggacc gagcacgact acggatccat gctccggctg gacgagttcg tcgacctcgt
     5701 ccggtcctcg gggttcgaga ttctccggct ccggaagccc gtgcccttcg gtctgttccg
     5761 gtacttcgtc ctggcggccc ggaaaacggg agcggcgcgg tgaccggcta tatgctcccc
     5821 gcggatccgt actatagtgg gcggcatgcg gggacttgaa gccgaattcc tgctcgatcc
     5881 cggaatccgt ttcctcaacc acggctcctt cggagcctgc ccgcggcggg tgttcgcgtc
     5941 ctacaggcgc tggcagctcc gcctggagcg ccagccggtc ctcttcctgg accgggagca
     6001 cgagggcctg ctgcgccgcg cccgggccgc cctgggcgcc tatctgaacg ccgatcccga
     6061 cgaccttgcc ttcgtcccga acgccaccca cggggtcaac ctcgccgcgg ccggtctgga
     6121 cctggggccc ggggacgagg tgctggccac ggaccacgag tacggggcct gcgacttcgc
     6181 ctggcaggac ctctgcggcc gcaggggagc cgtctacctg cgcaggccga tcggtccgcc
     6241 ctcccgaagc cgcgccgaac tggcggaggc cctgtgggcc ggcgtgacgc cccgcacccg
     6301 ggcgatcttc ctgagccaca tctcctcccc caccgccctg cgcctgccgg tggaggagat
     6361 ctgccgacgg gcccgggctt ccggcatcgt gaccgtgatc gacggagccc acgcccccgg
     6421 ccagatcccc ctggacctga cgggcctggg cgcggacttc tacaccggga actgccacaa
     6481 gtggatgatg gcgccgaagg ggtccgcgtt cctccacgtc cggcgggagc tccaggacag
     6541 ggtccgggcc ctcgtcgtga gctggggcta ccggaagcct ccgggaaccg gttccggatc
     6601 gaggttcctg gacctcctgc agtggagcgg aacctacgat cccgcggcct acctggccgt
     6661 gcccgacgcg atccggttcc aggcccggca cggctgggat tcggtgcgct cggagtgcca
     6721 tgacctgctg cggcggaccc tggagcggat cgccgacctg agcggcctgg agcccgccta
     6781 ccccccggac tcggacgcct tcgcccagat ggcggtgacc cccctgccgg agtccctcga
     6841 ccttgcggag gccaaggccc ggctctacga cgagtaccgg gtggaggttc cgctgatcct
     6901 gtggggggat cggaagttcg tccgcgtttc gatccaggga tacaacaccg aagcggacgc
     6961 cgacgcactg ctggccggac tccgcggagt cctgcggact tcccgagtga gcggatcgtc
     7021 ccccgcccgg ggccccggct ccgcgaggtc ctagggggcg ggggtccccg tccgccttcc
     7081 ccgcccgtac cacgggcgca tcaggccgga tccctcattt catgaacagg ttcgtcaacc
     7141 ttcgggtcgt agccgcaagc ccgaccgcga tcatcgcggc gaaatagagg acgtgccgga
     7201 gtatcgatcc gtcgaactgc cccagggtaa ggccgcggat cagctcgatc ccgtgccaga
     7261 gggggaaggc ctggatgatc catcggatgt actcggggta cacggtgatg gggtagaagg
     7321 tggcggagaa caggaacatc ggcagcatga agaaattgat ccaatccatc tgctggaagg
     7381 tggtcatgta ggaggtgagg gccatgccgc aggcggaaaa gccgaaggcg atcagcaggg
     7441 aagcgggcac ggccaggatg ccccaggcgc tcgggaccag gcccagggcg accatgaccg
     7501 ccaggaaggc cagggcgtag accagtccgc gcaggagtgc gtagaggatc tcgcccaggg
     7561 ctatgtcgag ggtaccgagg gacgtcgcga tcatgccctc gtagagccgg gcgaagcgca
     7621 tcttgaagaa cacgttccag gtcgagtcgt agatggcccc gttcatggcc gacgtggcca
     7681 gcagggccgg ggccaggaag gccgcgtagc tcaccggccg ccccagcggc ccggtcacct
     7741 cgcccaccag ggtccccagg cccagcccga aggccaggag atagaacacg ggctcgaaaa
     7801 agccggagac cacgacgaac cacgcggacg tcttcagggc gtacaaggcc cggaggacga
     7861 cggtgtgcgg acgcccggcg tagatccgcc gcatcatttg gacaacctcc ggcggaagga
     7921 tctccacgaa agaccgaggc cgacgcccag catccccgcg atgtacgcga cgtggacaag
     7981 cgtcagccag accggttcgg acatgccgta cgcggccgcg cgccccaact gcgtcccgtg
     8041 ccacaggggg gatatccagc ccagccaccg cagccagatc ggcagccctt ccaggggaaa
     8101 gaaggtaccc gagaacagga acatcggtgt gatgatgagc cggttgatga tcgggaacca
     8161 gccgtcgtcc tcctccaggt tcgccgccac cgccatgatc acggagccga acgccaagcc
     8221 ggacaggacg gcggccggca tggccaagaa accatagccg gagggggaag ctccgaaggc
     8281 cgccagacag caccagaaca cggccacggt aaaaacgatc cggaaccccg cggcaaggag
     8341 gacgccgttc accacttgcc gggcgtcgat cggagtcgcg ttcatggccc agaactcccg
     8401 cgtccacttg aagcccccca tgaccggata cgagcattcc tcgaacgcca cggtgagggc
     8461 ggcgctcacc aggagggccg gtccgacgaa gaccaggtat cccacgcccc cgatgcccgg
     8521 ggctccggcg ttcgagtcga tgtaggagcc gatgcccacg cccaacgcga acaggtagag
     8581 gacggggttg aacacgctgc cggcgatgat ggaacccagg tatgcccgca tcgtccgcag
     8641 gcgatgctcc gtcacgtacc aggttccgta agccatgctc cgggcgcttc ggcgccgccc
     8701 gaagtaggcc gacgcgccgg cgtgtacact gatcgcgtcg ctcattcgat caggctccgg
     8761 ccggtcagcc tgaggaacac gtcctcgagg ctggagcggc ggaccaggga ggtcagggga
     8821 cggaggcctc gttccacgat gcgggcaagg tcctcctcgc ccgtgtcggt gtagatgaga
     8881 attcgatcag gcagaacctc gatgcgatcg ccgaatcccg ccagctgtcc ggccaggacg
     8941 gcgtttctgt ccgaaccgaa gcggagctcc accacttcgc ggctcgaata ctcccggatg
     9001 agccgggcgg gggttccctc cgccatgatc ttccccttgt ccatgacgat gagccggtcg
     9061 cagagctgct ccgcctcgtc catgtagtgg gtggttatca ccagggtgac cccggcttcc
     9121 ttcaatcgga acagccggtc ccagagtatg tgccgggcct ggggatcgag ccccgtcgtc
     9181 ggttcgtcca gcaggaggat atcgggctcg tttatcaagg cccgggcgat ggtgagccgc
     9241 cgcttcatgc cgccggaaag ggtgtccacc ttggccttgg ccttttccgt caactggacg
     9301 aactcgatca gttcgtccgc cttgggcccc aggaatttca tgggcaggcc gaagtagcgt
     9361 ccgtagacga cgaggttgtc ccggacccgg atctcgacgt ccagattgtc ctgctggggc
     9421 acgaccccca ggtggccgcg tatatccggt ccgtgctcgt tgggatccaa cccgaggatg
     9481 gagagctccc cgcccgtccg cctcgacacg ctggctatca tgcgcatggt cgtagacttg
     9541 cccgcgccgt tgggacccag gaggccgaag gcttcgccct tccggacctc gaaatccacg
     9601 gagtccaccg cccggacctc gccgaaatcc ttggtgagcc cccgggcttt gatcattatg
     9661 ccgttaggat tgtccacccg cacaggatcc cgatttgagg tccgattgta aagccgtgcg
     9721 tggtccgccc cgggcctatc aggaatccgt ccggccgccc ttcccccgcc cggcctcgta
     9781 caggccgatc cagtcccgga cgctcatctt ccccaagagc cgggctatca ggtcgtgggg
     9841 gatctcgtcg tagtacttga accggacgca gctcttcccc atgtcgacct tgtgccttga
     9901 aaccttcgag tactcgatct tgaaccactc gagcaggtcc ggccgggcgt acatacccat
     9961 gtggtagaag ttgatggaac ccttctgggc ggccagggcc aggaagggca agggatcctt
    10021 ggggttacag tggtagcccg cggggtacag ggagtgggga accacgtagc cgatcatccc
    10081 gtattggatt cgttcctcca ggccctccgg cagtttttca aggatggtct gccggagttt
    10141 ttgatacggc tccttctgat cggaaggcag ggaatgcagg tactcctcga ccgtcatgac
    10201 ccctcctcgg aacgcgagtc gatgcattaa aatatattac tattttgata atattcaatt
    10261 cccgtacgga cccggacgtt ccggtacggc gcccagccgg atcacgtagc ccgccggcct
    10321 ctccgggttc cggtcgattc gccccccggc ctgctgggcc aggagggtga ccagctggag
    10381 gcccagggtt ttcggattct ccagggacag gtccgccggg aggccggggc cgtcgtcgga
    10441 atacttcagg ctccacccgg attccccgtc cgggcggatc tccagttcca tggcgcagcc
    10501 tccccggccc cgggcggcgt atttcaggga gttcgtgatg agctcgttca acgcgagacc
    10561 cagggggatg gcccgatcca tgcccaggga gaggggttcc ccggagacgg caaaccggga
    10621 cgcccccgcg gattccgcgt acgaggagat gaggctgccc gccagggatc gggcgtagtc
    10681 cgcgaggtcg atgtccgcca ggttgcgctc ctcgtagacc tccgcgtgca ccagggccat
    10741 actccggatc cggttctcgc tgtcccggag ggccgcccgg gcccgctcat cctccacggt
    10801 accggcctgg aggctcagga ggctcgcggt cacctggaag ttgttcttga cccggtgatg
    10861 cacctcccgg agcagggtct ccttctcgcg gagggaggcg gccaggcggt cctcgctctg
    10921 gcgcaccttg ccgtacgcca acgcgatgac cgccagggag ccggccagga ccaccgcgac
    10981 ggtgcccagg ccgacggtga tccagaaggc cgcggggtcc cgttcccgca gggaaagggg
    11041 gcggtactcg aaccgtacgt ccggggggaa gcggccggcg gggatccgga accgtcggag
    11101 ctgtcgccag tccaggacta cgggagtggc gggcacgtcg ctgaccggga tggctccggg
    11161 gctctcgccc cgcaggatgc gcagggccat gcggcccgcc tcccggccct ggtcgagacc
    11221 ggaggtcagg gcgcctccca cgatcccgtg gcccaggaag aagtcccaga tggcgaatac
    11281 gggacccgag gctgcggcgc cgacccggga cgccacgtcc cgcgcggaca ggacctcgcc
    11341 ggccccgtcc cgggcgtacg ccaagaggaa taccgccgtc gtatccggca gcccgaccgc
    11401 cgtccgcaaa acttcccgga acgggacgtc cgggaaggag gttatcggca ggggaaagtg
    11461 catggactcc gcggtcatga ggagctcgtg cagggtcttc ggtcccgtgg tggtccggtc
    11521 cgagaggact ttgacttccc gggtctccgg gaagagctcc aggatcaggc ggagggtgga
    11581 ctcgtaatcg atccgctcca ccaccccggt gacccggttc cgcacccggg cgatggacgt
    11641 cgcggagaaa tcgttcaccc ctatgaagat ccagggtacg gggccgaaga tcccgtcccc
    11701 gtgttccagc atgaactcga aggcgtcgtt gtccgagacc agcaccagat ccggcggaaa
    11761 tcccgagtac cggcgcgaaa ggtagcccgc gaaagcctcc gcccggccct cgtcccaggc
    11821 ccgcttggcg tccaggaact cggacttgac ctggcaggac aggtcccggg cctcctcgag
    11881 ttcctcgttg atcccctggt tgatcaggcg ggtccactcg tagtcgttct ggtaggaatg
    11941 gaggaccagg acctccttgg gcgagccctg ggcccccgcg tgatacggca ggaggagggc
    12001 caacagtgcg ggaatcgcgg cgcgccggat catgacgaca gtgtaaagcc tcccgggcgg
    12061 acggtcaaag ggccctgctc actccagggc cgcccgggcg gcttcccgga tctccgccca
    12121 caccggcccc agcgaggccg ccaggcgcac cgcctccgcg aggctctcgt ccttggccat
    12181 ccccttgccc gcgatgtcga aggccgtgcc gtggtccacg ctggtgcgga tgaccgggag
    12241 gcccacggtg acgttgatcc cttcgtcgaa gtacagggtc ttgaagaccc cgtgcccctg
    12301 gtcgtggtac atcgccacca ggaaacggta gattccccgg gccgcccggg ggaaaaggac
    12361 gtccgcgggc agggggccct ccacggggat gccctccgcc cgggcccgct ccaccgcggg
    12421 gaggatggaa tcgatctcct cggtgccgaa gaggccgccc tccccggcgt gggggttgag
    12481 gcccgccacg gcgacggtct cccctccccg gcccagggcc cgggccagga ggtgggccag
    12541 gcggatctgc gcgtagaccc ggttctcgtc gatcaggtcc agggcccggc gcagggatac
    12601 gtgggtggtg gcgtggaaga cgaaggtgcc ccgggcggag aggaccaggg agtagttctt
    12661 gaccccgaag ccctcggcca gaagctccgt atgtccgggg taggagaatc ccgcggcgtg
    12721 catggccgcc ttgttgagag gggccgtgac gatccccgcc acccgacccg ccttggccag
    12781 gtccaccgcg gccttcacga actccaccgc cccccggccc gcctcggcgg agagcttccc
    12841 gggcaccacc cccgaaaggg gggagccggg ctgatagagg gggatgcctc cgaactccgg
    12901 ggtccccggc cccaggccct ccatctcgcg gggggaaacc gggacgaaac tcggccggcc
    12961 ggccgccggc agcgattcca gggccgccgc catggaggcc cggtccccga tcatcagggg
    13021 ccggcaggtc tcccagagcc ccggccgggc cagggtcttt gcgcagatct ccgggccgat
    13081 ccccgcggcg tcccccatgg tcagggccag gaagggccgg ccgctcacgg ttccgccctc
    13141 gtgcaggtcg aaagaagggc cgcggccttt tccagggcgt cgggcgcccc gaagcccccg
    13201 gccttagtca ccaggaagag acccgggcgc tcgccgcccc ggaccgcgca gaggggaatc
    13261 ccgggggaca cctcccccag gaggtccacg gcccgggagc ccagggcgga caacagggcg
    13321 cgggccacgt cgccccccgt ggcggccacc cccgggtccg gaacatcccg gatcgcggcc
    13381 cggacagccc cggccagggc ccgtgccagg gccgcggcca cctccgcccc ggaaaggccc
    13441 cggccggctc ccagggcctg ggcggcttcc acgtccgacc gatcaccggg tgtggcgatc
    13501 aggaccttcc cgcccgtttg cagggcctcc cgggcccgga gtcccagggc ctcggccgcg
    13561 gcctccgcct ctgcggggga gcgggcctcc aggatctccg cgggatccag ccggaattcc
    13621 cgggcccccg cctcgatcag ggtccgggcc tggacccgag ccgcggggtt cacggacccg
    13681 accgccagga ccaccggccg cgtgcgggcg tccggacgga cggaaaccga gccggccgat
    13741 cccgccgcgg cgcccccggg acggcctcgg cccgcccggt cggcgaggat ttccgccgcc
    13801 ggcagggcca ggcccgcgga gcccgcgagg atcaccccgg accccaggga cagggcggcc
    13861 cgcgccaaca gggccaggtc cgcctcgccc ccggcgtcgc agaccgctat cccccctccc
    13921 gcacggcgga aatcctccag ggccgaacgg acggcggcct cgcccccgtc caggacggaa
    13981 cggccgagct cccggaccgg tcgccggctc tgacccacga tgtaggcggc tatccgactc
    14041 tccttgaccg ggttcaccgg atcccgggac agggccgtct cggccacggg caccccgtcc
    14101 acccggagga taccgtcccg gacgatccgc ttgttggccg ggaaggcggg gcagaggacc
    14161 gcgaggtccg ccccggatgc gtccattacc gcgtccaact cccggcccgc gggcccccgg
    14221 agggtggagt cgatcttctt gtagaccggc aagtaaccag ccgccgccag gacgccggcg
    14281 gccttgcgga cagcggcgta agccnnnnnn nnnngccgaa gctattcacg ggcgcatgcc
    14341 gccgggtcca ggggccggga atccgtgttg accaccaggg cgtcggatcc ctcgatatcc
    14401 tcgggggccc aatccccgaa aagggaacgg gtccgcaggc ccgcccgggc gaactgggcc
    14461 cccgtatccc cggctcccgt aaggtcgtcc gcgatcaccg cgtatttcat ggatgcctcc
    14521 gccatgggtc ggatcctggc acaggattcc gggtgcgtca aacggaaaat ccggcgctac
    14581 gctacacctg caccggtccg ccctctgcgc ccggcccgga cacccggccc cgcaaccatg
    14641 cccgtaccca gaacgccgcg tagaccgcca gcatcttgtc gatcaggttg gtcgggatcc
    14701 gtgcccagaa gttggcggtg aacagggaca gccccgtctc ggtgaacgcc gagacgatgt
    14761 tgtcgatctg gacccccgtg tccccggaaa agaccaggtt ggcgatgaag cctcccagca
    14821 aggcgttggc cagggtgacc aggaccaccg ccgtcgtcgc gtgccagccc tgcgagaagg
    14881 cccctctccg ggacatgagg cccacgatca ggcccgtggc catgttgcag gccgcgaagg
    14941 gccagtagta gcccgtcatt ccgtagatcg gctccatccc caggttcgtg gccaggccga
    15001 ttcccaggcc cgcccagggg cccaggaccg cccctccgac ggcggtgaag atggagtcca
    15061 gaaagagggg cgacttgagg accacgttgt tggtccagga gaagcccagg ttgaggaggg
    15121 agagggtaag gaccgcggcc gccgctgccg ggaagcccgg gcgttcgatg cggtcgaagg
    15181 gaaaaatcct atttctcatg aatccagtat atactattcc caaaatgatc cggaaatttt
    15241 cgccctacct ttccacctac tccaacggca tcctgatcat cctgatcctt ttctccttcc
    15301 tgggcatgac ggccctgatc tcccggtccc ggagggagac caccggcaag ctcctggcca
    15361 tggacgagct tttggccatg gaggcccccg ccccctcgga ctggcggagc gtcccggatg
    15421 ccctgtccgg atcccacccc ggccccgcgg gcagggagct ggaccgactc ctgggggtgt
    15481 accgtaccgc ggtcgccctc ctgccggatt cccgttcgga tcccggctac gccgaggcgg
    15541 agatccgcgt ccgccgactc ctctaccggg ttagcaagtc ctacaccgag cttctgcgcc
    15601 agcgggaagc ctccttcgac tccctcttct acttcctggc cgtcgggatc ctggtccaat
    15661 ccttcgcctt cctcctgtcc ctgtaccagg ttcgggtggc ccgggaggag ttggtccgcc
    15721 aggaccaact gttcaacctg gtccagaagg tccgggagga ggagcggcgg ggactcgcct
    15781 cctaactcca cgactcgatc ctccaggacc tggggagcct gggactccgg cccgccctcc
    15841 gggacgatcc cgaaggccag gccctcctgt ccgggagcat cgacaggctg cgacgggtga
    15901 cctacgggct ggcgcccctg cacctggacc aggcgggctt ggccgcctcg gtgcgggaac
    15961 tcgcggcgga acaccttcgc cggggcggcc ccccggtgga catcgaggag ctgggctggg
    16021 acgagtcccg gctggatccc gatacccgcc tggtcctcta ccgggccgtc caggaaggcc
    16081 tggccaacat ccgaaagtac gccggggcgg gcaaggtcga gctccggctg gtgggctccc
    16141 acccctacct catcctgagc ctccgggacg acggccgggg cttcgatccc cgttcccggg
    16201 gcggcccgac ctccccgggg ggcggcggcc tgggccttgc cctcctggag cgccaggtcc
    16261 gaagcctggg cggcgagtac tccctggagt cccggccggg agccgggacc cgccttcaga
    16321 tccggtgcga acccggaaag gagcgaccat ggaccccatc cgcgtccacg tgatcgacga
    16381 ccatgccctc ttccgacggg gaatcctgga gatcctgtcc aagtggcccg aagtccggtc
    16441 cgccggggag tccgaaaccg tggacacctt cctggattcg cccatgaacc aggccgtgga
    16501 cctcctgctc ctggacctga gcatggggga gggactcaac ctggaccgcc tgcccgagat
    16561 ccgggcccgg ctcccggagc tccgggtgat cgccctgacc atgcacaaca agcccgtgct
    16621 ggtgaagaag accgtcaacg ccggggtcca gggctacgtg gtcaagcagt cccctccgga
    16681 ggtcctacgg gccgccgtcc gccgcgtctc cgaaggcggc cgctacctgg accccgagct
    16741 ctccgagagc ctctaccagc tgatcctgga tcccagccgc gacgacgcct ccggctacgc
    16801 ggccctgacc ccccgggaac aggagatctt ccgcctcctg gccgagggcc tctcggcgga
    16861 gctgatctcc cgaaaactct tcatcagcaa gaagaccgcg gaaaaccacc gcttccggat
    16921 catgcagaag ctgggcctaa catccatcgc cgagctcgtg tcgtacgccg acgagctagg
    16981 ggtgatctga gggcgcccgc ctaaaatgag tctttctcct atggcttccg ggcacgaccc
    17041 gggtgtagca tgatcccggc ggtcgacgcc cagagaaaaa ccatccgaaa gatgatacaa
    17101 aacgattgga aaaagtcctt cacccggcca tgattcagaa cggcgcggcg gacaccccgg
    17161 gtgtttcgcc gcgccctctt tttgtcgggg gacagacgga accgaactct gcgctccaga
    17221 tactcaggac tccttcagcg tcgcagccag gaccgcggcc agggcgagca aggcgcacgc
    17281 cgcggccagg gagaccgtga acgaaccgtc ccgggtccgg agcgcctcca tcaggaacac
    17341 gaacaccacc gaggcctgcc cgaaaagctg tacgaggccg ttcgaggttc cctccggcgt
    17401 cggcaacgcg gcctccgagg cgtactgcat ccccgtgggc aggacgccgg tcaaaaagaa
    17461 gccgagcgac gcggcggcca ggcagagccc ggcgaaagat cgcgtccagg ccaaccccgc
    17521 gagtcccggg atggcgaggg tcgttccgag gaggatgaac ggccggcgcc gtccggtccg
    17581 gtccgagagg gccggcagga ccaccgcgcc cgccacgccc gcgacaagca ggacggctcc
    17641 cagggtaccc gcctgcacgg gcccgaaccc ccggggtcgg acgatggcct cgatccaggt
    17701 tgtcaccccg ttgaagattc ccaggccgat gaaggcgacg gccaggaagc gacggaagga
    17761 cggcacggtg aaggcgtgcc ttagtccgtc cagcatgagg gcacgctccg cgcccgcgtc
    17821 ctccgagggc gggaggggcg gccgctcccg ggcggccagg acgaagacga gggcgcaggc
    17881 cgcggagacg gtcccgtagt acgactgaac ccggggaatg ccggacgctt ccaccagggc
    17941 cggggtcagg accatgccca aggccgtgcc tgtaaggttg gccagggtga ccaagcccac
    18001 cgccgtggcc cgctccttgg gcgggaacca gagcgccgga accttggtcc aggcgttcag
    18061 gaggaagggc tgggccgccg cgatcccgat ggaccccgcg agggccaggc cgtagttcgc
    18121 tcctgccagg ccccgaagga tcccgaaggc acccatcatc agggagccga agcccacggt
    18181 ccggtggaag cccagggtgt cgatggacca ggaaacgggt atggacaggg ggatgaaggc
    18241 gatcatgaag accatggata gaagcccgat cttcaggtcc gataccccgt agaacgccgc
    18301 ggcaacgcct gtcaccgggg cgtaggagat ccaaaggagc tggacggtca ggttcaccag
    18361 catgaacgaa cccaacacaa cccatcgata cccgtagacc ctggtttcca tggccgcctt
    18421 cctcggtatc ccttaccggg accgggaagc atcctacccc gggagatccc gggtccgata
    18481 ccccgtcaaa agatcacgat gtcccgggcg ttcccggaga tcctggggag gaactcgcta
    18541 cccgagtaag ttggatatat ttctcccaag gtattgaaat tggtttcgac tttccccttc
    18601 gcgtacgaaa aagcttcctg gatcgtcacg tgaccatcac cgttcaagtc tcctttttca
    18661 gcagcctgca gaaagaagta cgtgaatact ccgtgtccgt atgtggatag ctctagggat
    18721 tcttcctgcg atccggcggc ggttacgact atcggatccg aaacgctgcc cgtcctgaaa
    18781 gcatcgccga acagcgtgga gtaattcttt aaagtggcca ggagcgtcga ggtagaactt
    18841 ccgttggtat aatccggatc cgctgcatcg gtggttcctg tattggtaac ggatactaat
    18901 ccaccagaat aacaagtatc taagatcgcg actttgttcc tgcacgaaaa agcttgcaac
    18961 gctgaaagaa tttcaccaga agaaatacaa cttgagtatt ctgtttctcc ttctccatcg
    19021 aaagtcgtat ctgttgggca tatataggta ttcccatcgg aatcaaaggt tccgtgaccg
    19081 gaaaaataca caagaatcga agagtcggat tggacatccg ggttgttagc cagatccatt
    19141 atatcttgaa gaaactggtc acgggtcact ttaggctccc tgatgactat aaccgaccat
    19201 ccggcagcct caagtgcagc tttcatgaaa atggcatcat cgtcagtata gttcaaatct
    19261 gtcgcgttat cgtagttttg tgccccgtac accagggcat acctcgtggc cgtgatctcg
    19321 aaggagcagg aggagagggt gaacagtgga caggtaaggg tgaacagaag gatctgaaac
    19381 aggcgctttc cggaccgctt cgggcgcgtc attcggaggc tcccgggacg gccgccccgg
    19441 accgccggcc cggcggcagg acgtagcctg cacccagccc cgcggaaaag gaccgggccc
    19501 cggggcggaa gatccaggag aggggcagga cggcccgcca gcccccgttc ccggacttgg
    19561 tgaaatccgc ccggaccgtc aggtccgcgg agaaggtcgc gaaggccacg gtggttccgg
    19621 gatagcgggc ggccccgaag gagaccccgc ccagcaggtc caatccgaga ggggcgccgc
    19681 cctcccggga gagcacgcgc gcccccgcac ccagggccag gtcccacccg gcaaaggaac
    19741 ggtacaggtc cccggcggaa ctgatcgcgg aggggaagac atggaagtac tccagttgaa
    19801 tccgaagggg aatccaggcc cccgcccgaa gctcggtccc cgtgccgaag gtgaaacctc
    19861 cgtggggcag gaccggggcc aggccgtccc cggacaggac cagcatgggc gcggccagga
    19921 cgtcgatgct ccaggccggg cggccggccg cgattccgcc gtcgtcggac aggacgcccg
    19981 cgggccgggc ggaagccgtc tgagccgcgg cgcccgacgc taggcagacg aacaccgcga
    20041 gcagggcgac cggcggatga agccgggcag cctcgacgcc ggtccgggac cggcgggaac
    20101 gggtcatgga ttcagtatat cgcttcgact accgaaaggc gaactccccg gtcaggattc
    20161 ggccgtcgct gtcagacctt ggggtaggcg gaggccaggg cggactccag gcggcccagg
    20221 gcgccagccc aggcggaggg gcccggggac atgaaggaga tccggaagct gcggcgggtc
    20281 gccgggttcg agccgaaatg ccgaccctcg gcccagggag gggacgccgg tgcggcttcc
    20341 cggtccgcgg tcacccagag gttccagccc gcggaggcgg gatcccggga cagccccgga
    20401 aaccgcgaca gcagggcgtc cagctcagcc cgacgggcag aggccgcgga ggcgcaggcg
    20461 gccaggtgtc gggcgtagag tcccgagcgg tagaagcggt ccaggaagag ctggagccag
    20521 gtcgccggcc ccgggtccga ctcggacttg aggttctcca gccgggtcag gacggagtcc
    20581 ggggccgaca ggagccccac ccggatgccc ggggccgtgg tcttggacag ggacttgatc
    20641 cagatggtcc gctccgggac caggtccgcg aagcgcgggg tcgaggacgg gtccgtctcg
    20701 gacaggtagt cgtcctccag gatccagacg tcccgccgcc gggcgaggtc cgccagggcc
    20761 cgcttgcgct cccgggaata ccggagtccc gtggggttgt ggaggtccgg gacggtgtag
    20821 aagacccgcg gcccgggacc cgcggacatc cagaacctgt cgaactcctc cggattcggc
    20881 ccctccgagc ccaggggcag gtgcccggag gggcggaaga gggccagggc cgcggggtag
    20941 gtgggctcct ccgccaggac cacgggacgg gcccggtccc cgaagagcct gcggcagagc
    21001 tcgagcccct gctgggcccc ggacaggacc aggagggact cctcgggcag gcggtacagc
    21061 cccgcaaggc tctcccgcag gccggggacg ccccggacgg gaggagccga gaatacccgc
    21121 cccctctcgg acttgagggc ctccgccagg aggtcccccg ccgggccgta ggggaagaac
    21181 tcctcggaca ggacggacac cgccagctcg ccctcccggg cctggcccga tccgggcggc
    21241 agggcgaagg acccggaacc cacccggttt tccgccaggc ccgcggcgct caggaggtcg
    21301 taggctttct gggcggtgag cttgttgcac ccgtacttcc gggccgcctc ccggatggag
    21361 ggcaggcgtt ccccgggccg cacctctccc tcctccacga gcctgcggag atccaggtag
    21421 atgcgctcgt agaggtgcac ggcgggccta gacggtcccc acccggaagg accggcggaa
    21481 ggcggtcctc cggaagcgct cggcccagac gaccaggatc gccatggcca ggcgggggac
    21541 ggtgagccag aagacgggga tcccgagggc cgcgtagagg atcgtgtcct ccaggaggct
    21601 gtgggacacg gcggcgtggt ggttgaagag gtcggcctcc tgccgtttca tcttaccgcc
    21661 ctcgatctgc tccatgatga ccgccgcccc gtaggcgtac ccgaccaggt tgatcaccag
    21721 ccagagcagg ctggccgagg cgggcaggcc gaagccccgc atgaaggggg cggccaggcg
    21781 ggccaggtac tccgcggtgt ggaactcctc caggagccgc tgggacacca ggatcgccac
    21841 gaccaggacg aagatcttga ggaccaaccg ggcggtcccc aggccccagg ccgccagggc
    21901 cgggggcaag gtcgaccaga ccgcgacggc ctggggggga ggggcgaagg aggccggggc
    21961 gggggagccg ggaaggagga ggttgaaggc ccagcccgcg gccaggccca tgcccaggcg
    22021 caccaggacc atcttgaggg ccgaggagcc ggccttccgc atgaccgcgg tctccacgat
    22081 caggttgtgg gcggtcaggc agacgatggc gacgatggag gcctcccgga cggggagtcc
    22141 caggaccgtg accacggcta tggcgctgta gttcgtgagc aggaatccgc tgaccagggc
    22201 cagggccgcc tccccgggaa ggccgatcag ccgcatggcc ggggccagga aacgggccat
    22261 atggtacagg agcccgctcc aatccaggag ggccacggcc agggacacgg ggatcatgat
    22321 cttgaccagg aaaatcgcgg tggaaaggcc ggttttcacg ccggagctcg cggatcgcgc
    22381 gatccgccgg cggatgctcg gttgttcatc catgggtggg tcgaattctc ccgagatcgg
    22441 catgccgtgt ctagtcccgg cccgcgtcgg ggccgccgag ggcccggtag aaggccagga
    22501 gctcgtcggg ggtgagggcc tccgcccggg cgtccgcggg aaagcccatg ccccggagga
    22561 tttccgccgc gtccgggccg gagcgtccca gggcccggag gttgttgacc gcggtcttgc
    22621 gtcgggagga gaagagggac cgcacgaagc gcgtgaagcc cggggtcccc gcctccgggg
    22681 ccgcgtcggc ccgggggctc aggaggacca cggaactggt gacccggggg gccggccaga
    22741 aggaggagga gccgatgtcg aaggcgatcc gcacctcgca gaccgagcgg cacaggaccg
    22801 tgaaggaggc gtagtccttg gtccccggac cggaggccat gcgccgggcc gcctccttct
    22861 gcaccgtgaa gaccatccgg gcgggaggcc gtccgccttc caggagggcg gccagcatgg
    22921 ccgcggccgc gttatacggc aggttcccca ggacccggtc cggccgggcc tcggccaggg
    22981 cggccgcccg ggtccggagg aagtccccct cccgcaggcg gaagccgggc agttcgccgt
    23041 agatctcccg gagggcggcg gagaagccgg ggtcgatctc gaaggcctcg acccgaaagt
    23101 cccgatccag gagctcagcg gtcatggccc ccaggcccgg cccgatctcc cagaccaagg
    23161 actccggctc ggcctcggcc aggtcggcga tccggcggcg cacgtcgggg ttcaccagga
    23221 agttctggcc gtagcgcttg cgcatcccca ggccccgggc ttccaggtag gccgccaggt
    23281 ccctcgggga atcgtagttc ggccgggagg cggcgccggg tccggtagag gtcgacatgg
    23341 ggaacagcat acacggccag ggccgccgcc gcaacgacgc aggcccaggc cccccggccg
    23401 gctccctccg ggaccgtcgg gccgggcacc caggcgaagg cctccatggt cagccggagc
    23461 gccccgtaca ggagtccgag gacggcccgc accggggctt ccaggatcgg cagggccgcg
    23521 caagcccccg cgcccaggag ccccgcccag atgaaggccg ccgtcagggg agccgccagg
    23581 gcggcggccg gaatccccgc cgggtagagg gtcccgaaga cggccagggt ccggggtccc
    23641 gcagccgcct gggcggccag ggaggcggcc agggccttgg ccgggacggg gggaacccgg
    23701 ggcaggatca ggtactcgaa ggccggggac aggagggcca ggcccgccag ggccaggtag
    23761 gagagctgga aggacaggtc ccgctccacc ccgggccagg ccaggcactg aaccaggaag
    23821 gccgcggcca gcccgttcgc cgcgccctgg ggcctgcccg ccagccgggc gatccccgag
    23881 taggcgaaca tgagggcggc ccgcaccagg gacggcttgg gtcctacgaa gaccgtgaag
    23941 gcgccggcca ggagcatcgc cgcggccagg gcgccccgcc ttcccaggag gggggccgcc
    24001 aggaaggaga ccagggcggc taggatcccc aggtgctggc ccgagagggc caggatgtgg
    24061 gcgcagccgg ccttccggaa ggcctccgcc aggtcgaggt ccaggtcgtc ccggaccccc
    24121 aggaccaggg cctccaggag tgccccgccc tcccctcccg cccgggacag ggcgtccagc
    24181 agtcccgccc ggattacggc ccttgaatcg acccagccgg aggcgtagcc ccgatcctcg
    24241 acgtcctttc cccgggcgaa gaggacccag ccggagccct cccggtccgg gacgagccgg
    24301 ccccggaccc ggatccgggc gcccgcgggg acgacggcgc acggccggtc gaggatcacg
    24361 gtcagcctcc ctcccgcgga agcccggaca tcggaacccg atcccgcggc ccggaggtcc
    24421 agggcgagcc gacggccccc cgtcctaagg ggggacgagt cctcggcggc cgtaccctcc
    24481 acccattcca ccaccccggc gttcaggccg aacgaagcgg gggcccccgc ctccagggcg
    24541 ctccgccggg ccaggaaggc caggaccagc gccgccgccg cggcccggag ccggtacggc
    24601 aacccgcctc ggccgcggga tcggggcggg ccgagggcgg gacccggtac ggggtgcgga
    24661 ccggaggccc gggtgaggcc ccggggcggg gcggaggccg cgagcacgac accggagagg
    24721 ccggcacccc ggcccgggcc ttgctccagg gcccgggccg ccccccagag ggccagggcc
    24781 gccggcagga acccggcggg cgcccgcccc ggaggcgcga ggaagaggca ggacgcgaag
    24841 accagggcgc agtcccgcag ggaggagtcg ggatggttcg tcacgcggag gataccgctc
    24901 ggagcgggcc cgggcgcttg cgccggcagg ggcgtccgag aaacctggag gcgccgaacg
    24961 aaggctcggg cggggagaac ggcagttccg gttcagcggc cggcgcggaa caatcctgat
    25021 tccgtcgcca ccaggggcac ccggacgtcc ctctcggtga cgggcaccag gggcaccacc
    25081 tgggtttcgt agcagaagcc gcaggggaca aagaaaccgg attccccggc cgtacccgtt
    25141 cccagggtcg caaggacgga agccaggaat cgatcgtaat agcccttgcc ccggcccaga
    25201 cggccgccgg tccggtcgaa gaggagcccc gggatcaggg cgaagacctt ccggcggcgc
    25261 aggtcctcca aggcgatcgg ttctcccgcg ggaggcgtcg ggatccccag ggagtcccgg
    25321 gggagggatt cccaggactc gtccaggtac gcaaaggcga tctcgccgcc ctcgatgcgc
    25381 ggggccgcga cggccttgcc cgcccggaga gccgcccgga tcaccgcgga ggtgtcgatc
    25441 tccgagggca tgctcaggaa ggcgaggacc agggaggatt cccggaaggc ctccatatac
    25501 aggatccgat ccgccgccgc gaggctgagg acgatccggt ccccgggact caccccccgc
    25561 aggagggccc gggcccaggc tcgggcctcg gccttgccgg ggaaggaacc cggggggctc
    25621 ggctcagcgg gcggcatggg cttggcggtt ctgttcctcg atgtccaggg cgtcccaggg
    25681 gtagcagatc caccggtcct cgagctcccg gccggccagg tagagcttga cctccggcgg
    25741 gatcacgccc cgcttttcct tgtccttgtt gtgcaggacc gccacggcca cggaggcggg
    25801 cttgtgggta aggagctccc ggatgcagta ctccagggtc gtccgggaat cgtccacctc
    25861 gtccaccagg aggatgcgct tgcccgtgag cttgcgctcc acctcgtcca cccactggac
    25921 cttatggggt gtgtccgtgg gcttgttctc gtcgtcgtag taggcgatcc ccacggcgaa
    25981 gatcggcttc ttgatgtagg tgcgcaggat ccgggccggg atgaagcccc cggacccgat
    26041 ggccacgatg gcgtccgcat cgtagccgct ctgccggatc cgctccgcca ggtcccggat
    26101 ggtcgaatgg atatcctcgt aggtgaacca gtacttctcc atggagggat gatagacacg
    26161 gcggccccgg aagcgcaagg gcggccggtg cgggcctgcg gccggacttt gctccgagcc
    26221 cggcatcctc tagcaggttg ttgaaatact cgaggcattt caacaacctg ctatgcaatt
    26281 gctcgagaac cttacggttc tctgcgcaat tgcgacatta aggaagtgtt gaaaaaggct
    26341 ctatagcctt tttcaacact cttctagggt tccacgagaa atccgaggtt tcctacggga
    26401 aacccttgac cctgacgcta cgtcagggtt tatggtcgga aacagccccc gagaccgggg
    26461 cgacgggagg tccccatggc gtacacggtg agcgagctgg ccgagctggc cggggtgagc
    26521 gtccgcgcgc tccaccacta cgaccggacg ggcctcctgg tcccggggag gaggagccgg
    26581 tccggctacc ggctctacgg ggacgaagaa ttgaggcggc ttcagcagat cctcctcttc
    26641 cgggagctgg acttccccct cggggagatt cggcggatcc tggatcggcc cgggtacgac
    26701 cccgcggagg ccctcgaggc ccaccggggg atcctccggg aaaaggggga gcggatcgcc
    26761 cgtctgctgg acctgctgga ccacacgatc gcgcagatgc gaggagagac gaccatgttg
    26821 acggacaagg aactgtacga gggattctcg gaggagagga tcgaagccat gaaggaggag
    26881 gcccggcggc gctgggggag ggaccgggtg gacgaaagcc atcgaagggt ggcggccctg
    26941 ggcaaggagg agttcgcccg gatcaaggcg gccggagagg ccctggaggc ccggttcgcc
    27001 gcggccttcc gggccgggga ggcccccggg tccgagcgga cccgggagat ctgccgaggc
    27061 tgggaggccc acatcgggaa cttctacgag cccggcccgg acatcctgga gggcctgggg
    27121 cgactgtacg cggagcccgg gcccttccgg gaccgctacg aggccctggc cccggggctg
    27181 gcggactacc tcaaggagtc cctggccctc tacgccgcct cccggcggaa ggactgagcc
    27241 ctcccggcgg gctttcggcc cccggccggg agcccgccgc ttcgttcccg ggggaaacat
    27301 cagtaccttt cccccaggag cgatcaacca tggaaacgac cctgtacgaa ttcaaggccc
    27361 gggccgcgga cgggaaggac ttcgacttcg aagccctccg gggccgtccg gtgctcatcg
    27421 taaacaccgc cagcaagtgc ggattcaccc cccagtacgc gggactggaa gccctgcatc
    27481 aggaattcaa ggaccggggc ctcgcggtga tcgccgcccc ctgcgaccag ttcgcccacc
    27541 aggagcccgg cacggacgag gaggtcgtcg gcttctgccg gcgcaacttc ggggtcacct
    27601 tccctatcct gtccaagctc gaggtgaacg gccccggggc ccaccccctg ttcgtctggc
    27661 tccgggggaa gacccggggc ctcctggggg accggatccg gtggaacttc acgaagttcc
    27721 tggtgtcccg ggacggcacg acggtgcgcc ggtactcccc ggccacggct ccggaagccc
    27781 tggaactgga tatcttgtcc gccctggagg cgcaacagtc ttcgtaccac taggtttcgc
    27841 cctcgggccg gccccggccc cgcttgacct cggaccgggc cttctttccc gacaggcgcc
    27901 gctcccggga ggcccgggtc ggcttggtgg gcctgcgctc cttgggccga tgggcggcct
    27961 tcacgatcag gctctccagg cgggccaggg cggcggcacg gttcaggacc tgggacctct
    28021 cgtcctgggc gtacaccacc aggtccccct ccgcggtcag ccggccgtcg aggcgctccc
    28081 ggaccctctc ccgctccgca tcggaaagcc cctccagctc cgctacgctc acccgggcga
    28141 tggccttcgt gctggtcttg ttcacgttct gccctcccgg accggaggaa cgggcgaaat
    28201 cgaggcgcgc cgcccgttcc agggaggctc tcagctgtcc aaggttcatc atccctcccg
    28261 ttccccggcc ggaacggccg ccctggtctt ccggggaaaa atgcgctaga gtgactatat
    28321 caaaaagtcc ggaaacggag aaacatgaag gtccggaaca acctggccca gatcacgaag
    28381 aagtcgctgt gcgaagcgat caatctcgac atgatgtccc gcctggcccg ggacgtcttt
    28441 tccgagtacg acctccacga gcgcaccggc ttcccctcca cggtccccat cacctcccag
    28501 ctcgcggccc accacatcgt caacgacgcc atcgcggaaa accggttcct gcacctggcc
    28561 gaacggctga tcctcctgga ccgggaaggc tacatgggcc gccgctaccc ggtccccggc
    28621 ctggcggaga tcgtcaagca gatccgggtg gagggttatg tctgggacga ggaggccgag
    28681 ctcttcatgg aggacccccg ggtgcgccgc acccccaact ggggccgcct cctggacgac
    28741 gacgagttcc gcttcgccct gctccgcctg gacgtggcca acaactccaa gatggtccag
    28801 acccacgggg acagcgcgat gcggggcgcc ctggaggacc tccggcagat cgtctcccgc
    28861 tgcgtggagt tccgggcggg ccggatctgg cagtgggagg gcgacggggc cctggcgggc
    28921 ttccttttcg gctcccgggt catgtccgcc gtgcttgccg gcatggccgt cctccacgag
    28981 cttttcctgt acgaccgcct gcacaaccgc ctgggggaac ccctccgcat ccgggcagcc
    29041 gtccatacgg gctccttccg gtaccactcg gacccggaaa tgctggtcaa gcaggagatc
    29101 gtccaggaaa ccgaggagat cgaatcccga ctcacccccg tcggatccct ggtgatcacc
    29161 ccggcggtgg ccccctccct ggaccgggtg atcctggaac gcttccgccc cgtccaacat
    29221 ttcgcggaaa gccgcctgct ggcctacgaa gtgcgcatgg aggggacatg agaatcctgg
    29281 tcttctccga aaccccggat cgggtggagg gcgccctcaa ggccgcgctc ccgaaggacg
    29341 cggacctgga aatcctgccc ctccggggcc tcaagcggac ggtccagaag cccctgaacg
    29401 ccgacctggt ctacctggac atcgccgaca tcggggaggc ggggctgggc aggtacgcct
    29461 ccgccctggc ctccgcggaa aaactgccct ggggggtcct ggataccgcg ggagagctga
    29521 ccgacccggc ccgggctttc ttccggggcg cccgggatta cctgggtccc ggtgcgctgc
    29581 gggccggaat taccccggcc cggctttcgg aaatccgggc ctttgcgggc ctggaatgcc
    29641 cgaatccggc ggccccggaa cccgcgaagc gtttcccggg ctgggacagg ctcccggagg
    29701 gctcggagat cgacgtgcgg ttctgctacg tctccgtggg ggatcccgag ggcctgcggg
    29761 aacgcatcgg cgaaaaacgc ctgcggaaac tgcgggagga tttcgcgggg ttcatggcct
    29821 cctgggcggc ggagtgcggg gggatcgcct ggatgcggga ccccgagggg accctcctgc
    29881 tgttcccgga gcgggacgag gggaccagcc ccgtgcttgc ggccttccgg ctgatcctgg
    29941 accgggccct ggtgggctac gaggtcttcc gcctggaggt ccccctgacc ttccgcttct
    30001 ccttccacgc gggccggacg gtctggcgcc gcccgggatc cacgggcacc atcgtctccg
    30061 gggacgtgaa cttcatcttc cacctgggag gaaagtactg ctccgacggc cggatcaccc
    30121 tgtccgcgga gaccgaggct tccatccccg cccccatccg ggacctcttc gtgcccgccg
    30181 gggacttcga gggccgtcgc atcctggcca gccgcaaatt ccgggattag gtttttcagg
    30241 aatcaaccac ggagaccacg gagaaagcga gttttggaac catagatcag acaggatgaa
    30301 caggatgggg aaaggatata caggataaag aaacggggga gaccatatcc cttacttcca
    30361 tcctgttcat cctctcttca tcttgttaat cctgtccttc ctatggttcc aggcgtcctc
    30421 ctaggcagcg gccgcccggt cacgaaagca ggtccctgtc ctccgcgtag tccccgcccc
    30481 gggtgcggcg gaacaggtcc accagccgtt ccacggtcag ccccttcttg acgtccccgg
    30541 ccacgtccgc caccacggtg ccctcgtgca tcatgatgag gcggtcgccc tcgtcgatgg
    30601 ccctccgcat gtcgtgggtc accgccaggg cggtcaggcc gtactccccc acgaagcgac
    30661 gcgtcaatcc ggagaccagt tccgcgttcc ggggatccag ggcggcggta tgctcgtcca
    30721 ggagcaggac cgcgggccgg gagatggagg ccatgaggag ggtcagggcc tggcgctggc
    30781 ccccggacag gcggttcacg ttctcccgca accggtcctc caggcccatg cccagctcgg
    30841 ccaggcggtc ccgcagctcc cgccggaggc gggacggcac ggcgatgcgg agcctccggg
    30901 gtcccttgcg ggacgcgagg gccaggttgt ccaggaccgt catgtccccc gcggtccccg
    30961 ccaggggatc ctgccggacc cggcccacgt agcgggcccg tttccactca gggaaccggg
    31021 actggtccgc cccgtcgatc aggatgcgtc cggactccag gggcaccgag cccgcgatcg
    31081 cgttcagcag ggtggtcttt ccggccccgt tggagccgat caccgagacg tgctgccccc
    31141 tgggcacggc cagggtgagc gaatccagga cccggcgctc gtccggcccg ccccggccga
    31201 agaccacctc cacggactcg aggcggatca tgcccgcctc cttccggccg gggtccggcc
    31261 cttgtaccgg ctggccgcga tggaggccac gatcaataca gcggtcagga ggcgcaggtc
    31321 gttgggggtg atcccggcca ggtagcccca ggaccggccc gcgtacatga tgccccggaa
    31381 aaggacggat cccaggagga cccgggccag ctggccgcct atgaggttgg tccggaatag
    31441 gaactccccg atcatgaccg aggccaggcc cgcggccacg atcccggccc cgaagttggc
    31501 gtccgcgaag ccctggtagg aggccgcgaa ggcaccggag agcccggcca gtccgttggc
    31561 caggcagatg ccgaccgtcc ggagccgggc gggatccacc cccgcggaca ccacggaggc
    31621 ctcgttgtcc cccaaggcgc ccagggcgat cccggcctcg gtgtggaaga agaggtccag
    31681 gaccaggacc agggcgcccg cgatcaggag gaaggccagg aaggcctgga tcgaagggtc
    31741 gaaggacccg cccagccagg actccgcggc ccgcatgacc gggttatagg cgatgagcgg
    31801 gaggttggcc cggccgccca ggacccgcag gtccacggag tagagccccg tcatggtgac
    31861 gatccccgcc agcaggggcg gaacccggag cccgtggtag acaccggagg tgacggcccc
    31921 cgccagggtt cccgccagga agccggctcc cagggccagg ggcgcgggga cccgggccac
    31981 gatcagggcg ctccccacgg cggccccgaa gggaaagctc ccctccaccg tgaggtccgg
    32041 gaagtcgagc acccggaagg agatgaagac gccgagggcc aggatcccgt agaccaggcc
    32101 ctcgaccaga actccctcga tcatccgtca cttccggcgg gcggcgccgc cctcgatcac
    32161 cagggaggcc cgggcctcca ggtcggcggg gatggagacc ccgatcgcgg cggccacgtc
    32221 cttgttcagg accagggcca tctcggaggg atccttggcg aaataggcgg ggatgtcggc
    32281 ggtcttctcc ccggccagga cgcgcaggac gatcttcccg gtggaccggc ccatggcgta
    32341 gtagtcgaag cccatggccg ccaggatcgg cagggtctcc gcggagctcg ggtccgcggt
    32401 cactaggggg agtttccgct cgttgcagat ctccgtgacc gcgctgatcg cggagaagac
    32461 ggtgttgtcg ttgcccaggt agatcccgtc cacgcggccc gcgatggaca ggagggcctg
    32521 gcggacctcg gaggagttcg tgaccgtggc gggcacgtag gacagcccct tctccgcgca
    32581 gtaggcctgg accagctccg cgagcttcac ggagttggcc tccccggcgt tgtacaggtg
    32641 tcccacccgg gccttcggct tcagggaaag gaggaggtcc agctgttccc gcacgggggt
    32701 catgtccgag gtcccggtga cgttcggccc gcccttgtcc agggaggcga ccaagcccgc
    32761 ggacacggga tccgtcacgg cgctgtagat cacggggatg tccttgatcg cgttggccat
    32821 ggcctgggcg gtgggggtgg cgatgcctac cgccaccttg accttggagc tcttgaacct
    32881 ctgggcgatc tgggccgcgg tgcccatgtc ggtgttggcg ttctggaggt cgaactggac
    32941 atccttgcgg gccgcggaga tctcgtccgt gatgcccttc tccagggcgt ccagggcggg
    33001 gtgggacacg aacttggcca cccccacgac gggggcgatc ttgggggagc aggaggacaa
    33061 caagacccct agggcggcca gggcgagggc ggacacggcg attcgtttca tcgagttctc
    33121 ctgggctgtt tcgactccgg cagatgcggc gggtcggtta tatgtttcta tcaaaagaaa
    33181 cattaaccca ggatagtgaa atacggaaag tgagtcaagc cccgcggaga tccgatcccg
    33241 ggtagcgcgg ttcggggaaa ggtgatatta tccgtagtaa catatgaaac gtgccgaaga
    33301 catagacctg atcgcccgga acctcaagga aatgtccgtg accctcgcgg ccgcccacga
    33361 tccccggttg atcgaggaat tcctgtacag cctgttcacg aaatccgagg ccgacgagat
    33421 cgccaagcgc tgggccctgg tgaaggacct ggcttcgggc atccctcagc ggcagatcgc
    33481 ccagaacctg gggctttcct tgtgcaagat cacccggggc agccgggagc tgaagaaacc
    33541 cgaatccgcc ttccggcgga tgctccagat cgcgggcatc cggatcgaag accgtaaaaa
    33601 gacccgggga accgcgaagg cgggctagga gcctgtcgga catgtggagt tttagcgccg
    33661 atcggcgcta aaactcccga ttatccggct cctttggagt ttgcaaggta gaaaaatcgc
    33721 gtatatgcga tttttctatt gtttaggtgg gcaagcccga caaactccta gaccgggcta
    33781 ctcggcccgg cggctccccc cgttcaatcc acggcctcga tccggatccg ggatcctccc
    33841 cggcccttgg ccacctcgat gcgggccggg atccggtccc ggagttcggg gacatgggag
    33901 ataagcccca ccatccgccc ctcccgcacg cggtccagga cctcgatcgc ccggtccagg
    33961 gcctcgtcat ccagggatcc gaagccctcg tcgatgaaga ccgcgtccag gacgatgccc
    34021 cccgcccgcc gggtgatcac gtccgccagc cccagggcca gggcgatgga ggccaggaat
    34081 ttctcccccc ccgatagggt gcccgcgggc cgggaccgtc cggtgaagga atcgtggacc
    34141 gagaggtcca gacccgcctt ggcggtgccc cgggcgggtc cctcgtccac ggacagggaa
    34201 tagcgccggt ccgacatgtc caggaggcgc cgggaggccg cccgggccac ctggcgcagg
    34261 tacagggaca gggcgtagtt cttgaagggc agacgggagc cggacagctc tccgttgagc
    34321 agcctggaaa gcccgtccag ggtccggccg cgctcctcca gggtcctccg ctcctcggag
    34381 agtacccgcc gccgggactc gccccgccgg gcggcctccg ccctccccga ggaggccagg
    34441 acctcggccc gggcctcctc gaggcgggcc tccgcggcct ccgcctcccg ggccagggcc
    34501 tccggatccc ccggctcccg gccctccgcg gcctccccgg cagcccgggc ccgggcccgg
    34561 gcctccgaca gctcgcggtc gtagcgctcc gcctcggagg cgaggcgggc gcggtccggc
    34621 ccggaaagga gggccgtccg gcactccgcc ccgtcccgga atcccgcggc ggccagggcg
    34681 gatccctccg cctcccgggc cgtttccagg gcggcctcca gggcggggat ccgttcctcc
    34741 gccaccgccc ggcgggcccg ggcggatgag agaccgtccc ggtggttccg gacctcctcc
    34801 tccgcggcct tccgggcccg gtccgcctcg gcccgttccc tccggacgag ctcgaggcgc
    34861 tccgtagggt cctcgtcccc ggcggacgcc cgggcttcgg attccgccgc ccccgccgcg
    34921 gcagccgacc gggacgccgc ggaaagccgc tcccgcgcgg atcggaggcc ctcggccaag
    34981 gcttccgcga ccgaccgggc ggccgcggcc gcacgggccc gctccgccag ggcgcgcaga
    35041 gcctccacgg ccgcggattc ctccgcctcc gccgcggcca ggagctcccg cgcggcctcc
    35101 tcctccggga catttcccag ggcggccagc tccgcctccg cctcctccgc ctcccggcta
    35161 cgatcccggg cccggccccg ggccagggcc tcccgggccc gggccgcgtc ccgggagctt
    35221 cccgcctccc ggagccggtc ctccaggccg aaggcctcgg ggcgcggcgc ggccggcgcg
    35281 ggatggtccc gggacccgca gaccgggcag ggctcgccgg gttccagtcc cgccgcgagt
    35341 acgccggccg cggagagatc cctctcccgg gcgaggtccg agagccgggc ctccgccacc
    35401 tcggcgtccc ggacggcctc ctcggcctcc cggtccttcc gggccgcctc ctcccgggcg
    35461 gcgtcccgct tccgggccag ctcggccccc cgggaagccc ggtcccggag cttgcgcgcc
    35521 tcctcccggg ccgtccgggc ggtttccctg cgttcccgtg ccccttcctc ctcctccggg
    35581 gagagggcca ggccgcggta cccccgctcg gcctcgtcca gctcccgggc ccggtcctcc
    35641 gcctcggccc gggcggattc ctccgcggcc ctcgccgctt ccccggcggc agccgcggcc
    35701 cgggcccgtt cccaggccgc ccgggccgcg gccagggctc ccgcctcggc gtccagggcg
    35761 gccgcccggg cggccaggtc ctccgccttg cgggccgatt ccgccgcctc gccctcccgg
    35821 gcctccaggg cctccacctc cgcccgggtc ttccggagct cctcccgtgc ggtctcgagg
    35881 tccgcctccg ccttctcccg ggcgtccacc cggggggccg cggagcgcgc cgcggagtcc
    35941 cggtccaggt ccctccccca agcctcccgg gcgggccggc ccgcctcccg ggcggccagg
    36001 gcccgggcct cccggtccct ccgctcgagc agggcctccg tctcccgggc ttcccggaac
    36061 gcggccttcc gggtctccag ctccgcctgc gcggcgcgct cccggtcccg ggcgtccgcc
    36121 agggccttct ccaggtccga aaggagggct ccggcgctcc ctccctcccc gggaccgccc
    36181 agctcggcgt ccagtcgggc cagttctccg tccaggtatt ccaggcgcct ccggacatcc
    36241 gcggcccggt ccttggccag ggcggctgtc cggtcgtgga gatccacggg aaagagcttc
    36301 tggaggaccg aggtgcggtc cgtggagttc atctccagga agcgttggaa ggccccctgg
    36361 ggcaggagga cgatcttggt gaactcctcc tgggtcaagc cgatgcggcg ccggaggatc
    36421 tcgtccacct cggagatccg gtccgagacc aggcgggccc cctccccgtg gccctcatac
    36481 agggcggccg cggcgggctc ctccacggcg gaaccggcct tggtcgcctt cttggggggc
    36541 ctgcggaagg gaggggtacg gaccacccgg aaggcgtcca gcccgagcca gaactccagc
    36601 tccacccggg tctcctcctc cggcagggcc aggtgggatc ggagctcccg ctccaggctc
    36661 ccccgggact ccggggcccg gccgtagagg gcgtaggtca gggcgtcgaa gatgctggac
    36721 ttgccggagc cggtcttccc gcagaccagg aagacgtccc ccaggcggtc gaagtccacc
    36781 tccgcggtcc cccggtaggg gccgaagttg gccaggacca gcctacgggg ccgcatcggc
    36841 cgcctccgaa aggagctccc ggaagagctc caggaccggg ggctccgggt ccgccccccg
    36901 caccgcccga tggaaggcga ggaagtcctc cagggccgcc ccggctccgt cccgggaggc
    36961 ggacgccctc gcgggggcct cggcggcctc ggcctctcct aaggagggat tctcgaaggc
    37021 cgcctggcgc agggacagga ggttcgggaa gacccggcgg agggattcca cgggggagac
    37081 gacgggttcg ggatccgtga ggaggatttc caggtaatgg tccttctcgg cccgacccgc
    37141 cccggattcc aggaggtccg cgtagctccc ctccagccgc gacagggggc ggaggggctt
    37201 cacgggcacg gggaccgcct cgaaccctcc ccgccggacc tcgaccagga ggaagccctt
    37261 ctcctgcccc gcctcggaaa aggagtagcc caggggggaa cccggatagc ggcccttggg
    37321 gcccgccttc tggggacggt gcaggtggcc cagggcgacg tagtcgaagg gctcgaaggc
    37381 gtcggcgtcc accagttccg cggagcccag gaagacccgc tcggattcgc tggccagccc
    37441 ccccgccgcg aaggcgtggc agaccaggac gttggccccg tccccggcaa ggcgcgggac
    37501 gatgaggtcc agggcggccc ggaagcgccc ggactgggat ctcggcgcgt ccggaaaggc
    37561 tccggccccc aggaagggca gggcccagag atacaggcgt tccccgtccc gttccaggac
    37621 caccggttcc gcggcgtcct ccggccgggt ccggacgtgg acccccgccc ggcggaagag
    37681 gctgtccccg aagcccaggc gggccgggga atcgtggttc ccaggtatga cgaggacgcc
    37741 caggtccggc cgggcctcct tgacccggcc caggaagttc ccgaggaggc tcaccgcctc
    37801 gggcggcggg acggccttgt cgtacacgtc cccggcgatc accagggccg ccggatcgtg
    37861 ctccacggcc gcggccagga gctggtccag catggccgcc tggtccggga cgaggtcccg
    37921 ctcgtggagg gtcttgccca ggtggaggtc cgaggcgtgg aggatcttca tgccgagggc
    37981 tgccgggggg ctcctcgtcc ggacagatca aaggaagcct cccagtcgga gacgatccgg
    38041 gcgacgtcct tccggaccgc ctcgatcccg tccgcctcca cgcggcccgc ggcctcccgc
    38101 agtcgttctt gggcggagtc ccgggccttg cggaactcgg gatccgggga gagggctttc
    38161 ccgccccgcc ggtagaggtc ccggccccgc ttccagtatt ccagggaggc cggggcgtcc
    38221 tcggagtaat cgtagcccgt ccacaggggc gtgaattcgc ccttcgaaag gaggacgggc
    38281 ttgtacagcg agatgtccgg gtaggaggtt ccggtgaacc aggcgacaca gcggcccgcc
    38341 cgcggatcgt actccaccgc cagggacgcg gtggtcgggt agcccaggtt gtccccgtcg
    38401 tgggcgcaga tcaccgcggt cttgcccggc cgggccgggt ccgggaggga ccgcgaccgc
    38461 agggtgtcga ggactacggc caggtcgagc ccgggtcccg cggccgccag gcgcccctcc
    38521 acggcccggc gccgggagtc cccctgggtc aggaaggcgt gcaggcggct ttccacgtaa
    38581 ggtttccagg actccttttc gccgatccgg cccgcgtcgg cctcgtcgta gcaggccatc
    38641 ttctcgttca ccggggagat ggccttccgg gtctgggcgt ccaggcgttt gtagtccagg
    38701 ccgatggagt aggagttgga gatcgccgaa ggtccctccg ccttcctcca ggcccagcgc
    38761 ttccccgcgg tctccaggac gaaggccccg tcgaagcccg ctgccaggta ggaattatgg
    38821 tagaacagcc ggccccggta agccccgttc ccgccctggt cgaagcgctc ggtgagctcg
    38881 accaggatct ccaccgcctc ctgggccgtg gaggcggacc ggagggcgat ccggaggaag
    38941 tccatcccca ggataccgtc cttccgggcg ggccacttgg agaagaccgc ctcgttgcct
    39001 atggcgacac ccttctcgtt gaggccgatc tcggcacccc ggatccagga gggcttggat
    39061 aggaggaccg cgtagccctt gtccgggtac ggaagctcgc cgccggacac ggcggaccgg
    39121 ggagcttccg gagcccgggg caggatacag acggcctggg gctcggcggg cttgcggtcc
    39181 gagttcttgc cgaagtacga gaggcccgcc ccgggggtcc agaaggtgtc gcacatcgcg
    39241 gcgtcctcac ttcaccggcg tcagggactc ggcccgccag acccacttgc ccttgtccgc
    39301 gtccacacgg accaggaagc agtccgaacc gtcccctttc gtgccgacca gctccgtgat
    39361 ccgggcggtc atgcccacga aggcgtccat ggaggggttc cagttctcgt tcccgtccac
    39421 gaagcggtgg cggcccagga cgacacggtc gcccacctgg aatccgtagc tcccggcgac
    39481 tccccggccg acgacggcca tgttccggat ccgccaggac caggtgttct ccttaacccc
    39541 gacgaccagg aaaccctggg aatccgcccc gatgatctcg gtgaccgtgg cctgcttgcc
    39601 cacgaaggcg tccatctccg gggcccagtt gtcgccgccg ttccggtcgt cgtggcggcc
    39661 caggatcacc ttggtgccca ccccgaccat gcccgagagg tcctcgccca ggtcctcgta
    39721 ttcgccttcg ccctcgtcgt cgtagacctc gccgccctcc cagtacccgg ggccctcctc
    39781 gaggagctcc atgtccgaaa ggcgccagta gaaggtcccg ccgtccacgt ccaccagggc
    39841 cgtgggctcc ccccagggac agagtcccgc gtgctcggtg atgtaggcct ccatgcccac
    39901 gtactcgtcc atctcggggg cccagtacgc ggactcctcg tcctcgggat ccagctggag
    39961 atgccgtccc aggaccacga gggaacccac ggggaaggcg tcctccaggg cgtagtcctc
    40021 gtcgtcgtaa tcctcgtagg aatagtcccc gtccccgtag tcctcgtcgc cggagtcgac
    40081 cacctcgccg gttccccgct ccaggagctc catgttctcg gtccgccagt agaactggcc
    40141 ctcgtcgacg tccacggaca cgaccggccg gccccaggga tcctgcccct ggtggccggt
    40201 cacgaccgct tccaggccga cgtagtcgga catctcatca ccccagttct tggagtccgg
    40261 atcggtcttg gacagggcgg catgctctcc caggaccacc cgggaaccga tcgggaagag
    40321 ggtctcgtag gaggcgctcg tcctccactg ctccgccagc ttggggaaat ccgccagggc
    40381 tttctccccg accggctcca cggcgtcggg gtacacgaag tagactcgcc cggacaggat
    40441 gcgggggaag ccctccggga gggcctccac ccagggcgtg gcgtccaggt ccttgtccca
    40501 gaccaccagg atgggcggat cctcctcgtc cagggcgaag aaccagccca ggtcgccctt
    40561 ggcgaccttc tcgtagtcct ggaccgcccg gacacgctcg cccttcggga aggcccaggc
    40621 gatgtagcgg ccgtacgcgg aggaatccgg gaaatccgcg agctttagga aggaggtgct
    40681 gaccacggcc cagccgccgt ggagccagcc ctggaccccg tcgggggtgg agatccggag
    40741 ccagcggccc cggcggcccg ctacggcctc ctcccgggcg ccggtctcca ggacccggaa
    40801 ggaatccccc gcggacaggg tggccgtcac cttggcgttg gcggaggccg cggcgaagac
    40861 cttcgcctcc cccagggccg ccccggtcgt gccggggggg gccgcgaagg ccagggaagt
    40921 tcccaggacc aggagggcga agaacacgaa accggcccgc atcgaacgtt tcatggctga
    40981 ctcctcgcga acggcggggt ccggccggga tcggccccat gcatgcatgg ccccgttgct
    41041 acgaatatat gacaagcgcg gccggaatac gagggaaaaa cggggccgag tacgtcagcc
    41101 cttgcggccc ttgagcagtt ccgcgaaggg gttgtaggtc gtgccgtcgt cgtcctccgg
    41161 cttgggcccc cgggcggggg tccgggcgga ctgggagtag gggttgcggt cccgtccgga
    41221 ccccgggccg ccgccgaaac cgtcccgccg ctccggccgg gccgggccgt cccggtcctg
    41281 ggcgcccggc ttgcgcacca ccgcgccgcg gcgcgccccc gccggggcgg ccgcggcggc
    41341 cctgtccgtg cccgcgccct ccggaacccg tcccgccccg gccggatgtt ctcccgcggc
    41401 cccgcgctcc ggggagggac cggcgccccg gcctcccctc gcggcgcctt ccttccgcag
    41461 ggacaggccg atgcgcctcc gctcgggatc cagggaaatg atgcggaact ccttcacatc
    41521 ccccaccttg aggacctcca tggggtcacg gacgaagcgg tccgccacct cggagacgtg
    41581 gatcagggcg ctctccttga tcccgatgtc cacgaaggcc ccgaaatcca ccacgttctt
    41641 gaccttgccc gtgacggtca tgccctcgga caggtcctcg aactggagga cccccttctg
    41701 gaggatgggc ttgggcaggt cctcccgggg atcccggttg ggcttcttca gctcccccag
    41761 gatgtcgtcc agggtcgtat ccccgacctt gtacttctcc ttgaggcccg cccggaggtc
    41821 cttggtcagg tcccggccct cccggaccac cggcaggatc tcctcggcca ggggatagtt
    41881 ctccgggtgg acccaggtgt tgtccagggg gttggggctc tccgggatct tgaggaagcc
    41941 cgcgcactgc tcgaaggtct tgtcccccag gccggggatg cccttgagct ggtcccggcc
    42001 cgagatccgg ccgttggcct cccggaactt gacgatcttc ttggccaggc ccgcgttgat
    42061 ccccgagacg tacttgagga ggctgtgact ggcggtgttc aggttcaccc ccacgttgtt
    42121 caccacggag ccgaccacct cgtccagggt gtccgagagg cgcttctggt tcacgtcgtg
    42181 ctggtagagg cccacgccga tgctcttggg atcgatcttg accagctccg ccaggggatc
    42241 ctggaggcgc cggccgatgg agatggcccc ccggatggtc aggtcgagct ccgggaactc
    42301 ctcccgggcc acgtcgctgg cggagtagac cgaggcgccg tcctcgtcca ccaccgtgta
    42361 cgccacggaa agcccgttct ccgagatggc gtccgccacc agcttctgga cctcgtggct
    42421 ggccgtgccg ttgcccacgg cgatcagggc cagggagtgc cggcggaccg cctccgcgac
    42481 ggccttcttg ccctcctcgg gcttggtctc ctggttgatc acgaagtagt ccaggaactt
    42541 ccccgtctcg tccagggcgg cgcacttggt gcccgtccgg atcccgggat ccactcccag
    42601 gacccgggtg cccttgatgg ggggctggag caggaggttc ttgaggttct cgctgaacac
    42661 gtcgatcccg tggccctccg cggcgtccga ggcgtcggaa cggatctccc ggaggaccgc
    42721 cggggaaatc agcctgcgca ggccgtcgtc cagggcgtcc cggtggtagg tcgtccgggg
    42781 ggcgtacctg gccttgagcc gctcggcggc cccgtcctcg tccacgtcca gggtgaccgc
    42841 caggaccccc tcccgctccc cgcggttgac cgccaggacg cggtggggct tcacccggga
    42901 cagctcctcg gagtactccc agtacatctg gtagacgctc ttggccttgg ccgactcgtc
    42961 cccgactccc ttcaccgtca ggcgcccggt cttcaggtac caggacttca ccagggcccg
    43021 gttttccgga tcctgggagg cacgctccgc caggatgtcc atggccccct ggagggcgtc
    43081 ccgggccgtg ggcaccgaaa gctccggatt ctcggggtcc tcccggacga actccggggc
    43141 ccgggcctcc accgccgcgt cgtccccgga aaccatgagc tccgccaggg gctccagccc
    43201 ccgctcctgg gccagcatgc cccgggtctt cttctttttc ttgaaggggg cgtagatgtc
    43261 ctcgagctcc gcgaggctgg aacacttgag gaggttgtcg tggaggtccg cggtcagctt
    43321 gccctggccg aagatcgccc ggacgatctc caggcggcgg gtttccaggt tgacgtagga
    43381 ctcgtacttg tggaccgtgt cccgtacctg gacctcgtcc aggctgcccg tccgctcctt
    43441 ccggtagcgg ctgatgaagg gcaccgtgca gccctccgcc gtcagggaca ccacggccga
    43501 cacctggccg gtccgtatcc ccagctctcc ggcgatccgg ttcatcaccg ccacttcgtt
    43561 cacggaaagc ccgtccacgt actcctggtt cagctccatg tgctctcctt gggatgctcg
    43621 cgtcgcggcg ggtacgatac gggtttggca tggaaaaatc aacaaggttg gagtacactg
    43681 gggcacggga tcgtgaaaat cgaggcatcc tctattcttg cggctgattc ttgacattat
    43741 ggatatagtg actataataa aattgatatt tatagaataa gtaattaggc gccgacggcc
    43801 gtgccaggag ttgttatgcc gaaagcaacc gagaaagtca ggatcttctc cgccctggaa
    43861 gtcgcgaatc tctgcggcgt cgtcaaccag acggcgatca actggatacg caacggtcac
    43921 ctcaaggcct tcaccacccc gggagggcag taccgggtct acgccgagga tctccgcgcc
    43981 ttcctcgaag gccggggcat gcggatcccc gacgagctct ccggcaagtc ccggttcgac
    44041 gtggactgga actccctggt gatcatcgac gacgaccggg aactcaacga cctcctccgg
    44101 cggtacttcg agaagaagct tccctccctg gcgatcgccc aggccttcga cgggttcgag
    44161 gccggacgga tcctgacgga aaagcggccg ggcttcgtca tcctggacgt ggacctgccg
    44221 ggagtcaccg gccactccct gtgcaagcgc atcaagaccg atccgagctt cggcaaaccc
    44281 tacgtggtgg ccatcacggg cctggaccgg ccggaggagc gaagctccat gctggaggac
    44341 ggcgcggacg ccttcttccc caagcccctg gatttcgagg aagtcaccgc ctccctcctg
    44401 gagttcgccg ggaaagtgag ggcccccgtt gcagccggat aagacccgtc ccgccctcgt
    44461 cctggtcgtg gaggacgagg actcgatccg cctctccctc cgggatttcc tgcgcggccg
    44521 gggctacgag gtgctggtgg cttccgacgg ggtcggggcc atcaagcagc tcctggacaa
    44581 cgacgtggac gtcatcatca gcgactaccg gatggaaacc ctcgggggcg actactgggt
    44641 ccggttcctg gcccggtact gtcccgagaa gaagatcatc ctcacctccg gtttcctgag
    44701 gccggacttc aaggtgccct tcgaggtggt ctacaagccc ttcgactact ccgaactgga
    44761 agccctggtc ggccgcctcg cctcggaggc ccgggacgcc cgcaggccct agccgcgccg
    44821 tccgggccgt ggtctccggc cgggtccagg gcgtgggatt ccgctggtcc gcccgcatcc
    44881 aggccctgcg gttccacgtg tccggctggg tccggaacct tccggacggg gacgtggaga
    44941 cccacgcgga gggcagcccc ggggccgtgg aggaatacct ggcctggctg gagaagggcc
    45001 ccccctacgc ccgcgtggaa tccgtccgtt cccgtcccgt cgaatgcagg ggatacgtct
    45061 ccttcgatat cgaagactag caggttgttg aaatactcga ggcatttcaa caacctgcta
    45121 tgcaattgct cgagaacctt acggttctct gcgcaattgc gacattgagg tagtgttgaa
    45181 aaaggctcta tagccttttt caacaccctg ctagatccgt ttgaagatca ccaccgcgtt
    45241 ctgtcccccg aaccccaggg aatccttcat cgccgcgcgg atgggcgcgg aacggccccg
    45301 gttcggcacg tagtccaggt cgcactcggg atccgggttc tccaggttga tggtcggcgg
    45361 ggcgaactgg tcccggacgg ccaggaccgt ggctaccccc tccacacccc cggcggcccc
    45421 tacgcagtgc cccgtcatgg acttggtgga ggagatcatg agcttccggg cgtggtcccc
    45481 gaaggcgagc ttgatggcct tggtctcgat gggatcgttg aggggggtcg aggtcccgtg
    45541 ggcgttcagg taatcgatgt cctcgggtcg catccccgcg tccttgaggg ccagggtcat
    45601 ggcccgggcc gcgccgcggc cctcggggtc gggggcggtc aggtggtggg cgtcgcaggt
    45661 ggtcccgaag cccgccagct cgcagtagat gcgggcacct cgggccttgg ccctctcgaa
    45721 gtcctccagg accaggaccc cgcagccctc ggccatgacg aagccgtccc ggtccttgtc
    45781 gaagggccgg gaggcgtggg cggggtcgtc gttgcgggtg gagagggcct ggatcatggc
    45841 gaaggaggcc accgccagcc gggtgacgca ggcctcggag cccccggcga agaccacgtc
    45901 cgcgtggccc tcctggatcc atcggagtcc cgtgccgatg gcgtccgtgc ccgcggcgca
    45961 ggcggtcacg atgcactggc agggacccgt ggcgcccacg gagatggcca cgttggcggc
    46021 gccgatgttg ggaaggacct tgaccaccgt caggggcggg acccgtcccg ggccccgctc
    46081 gtacagacgc gccgcgctct cctcgatgga ggaggtccct ccctgtcccg tgcccaggca
    46141 gactcccaac cgctcgggat cctggacacc ctgccccaga cccgcgtcct cccaggcctc
    46201 ccgggcggca tagaccgcgt attggctgaa gcggtccatc ttcttggctt ccttggcgtc
    46261 catccgaagg cccgggtcga agttcttgac ctccccggcg atcttgacgg acatgtcctc
    46321 gacgttgatc tgggtgatgg gaccgatccc gttcctgccc gacttgatgg attcccagaa
    46381 ctcggggacg ctgttcccga ccggactcac gacccccaag cccgtcacga caaccctgcg
    46441 tctatccatg gtccaccccc tgaaaccgta gaagatggac agattctacg ctactcggcc
    46501 ccccgcttcc agaggccgtg cccttgctcc tgaagatcga cgaagccgct gcgcggcttc
    46561 gttgccggcc tggccattcc tgggcgtcgc ggaagcccga agggcttccg tgcccttgtt
    46621 tcgggaattc gacgaagccc gcaagggctt cgtcgccggc ttggccattc ctgggagtcg
    46681 cggaagcccg aagggcttcc gtgcccttgc gatgtagcgc gttatcgcgc tacatcgcaa
    46741 ggccgccgtc caccttcaga acctccccgg tcacgtagga ggccaggtcg gaggccagga
    46801 agaggacggc cttggccact tcctcggggg agcccgtccg gcccaggggg atggccgcgg
    46861 ccagcttggc cttggcctcc tcggggatcg cctcggtcat ggcggtgtcg atgtagcccg
    46921 gcgccacggc gttgacccgg accttccggg cggagacctc ccgggccagg ctcttggtga
    46981 agccgatgag accggccttg gaggcggagt agttcgtctg gccgccgttc cccatgatgc
    47041 cgaccacgct ggagacgttg atgatgctgc ccgaccgctt cttgatcatg tgccgggccg
    47101 cggtgcggga aaccaggaag gcgctggtca ggttggtgcg gaccacggtg tcccactcgt
    47161 ccagactcat ccggaagacc aggttgtccc gggtgatgcc cgcgttgttg accaccacgt
    47221 cgatgcggcc ttcggaggcc gccacggcgt ccacggccgc ctcgaccccc ttctcgtccg
    47281 ccacgtcgca ggccgtccac gccacagtcc cgccgccgga agccgccagg gcctccagct
    47341 ccgcgtggtc ctccgccttg gtccgggaga aatagtggac ccgggcgccc tcccggagga
    47401 aggcggccac gaccgcgtga ccgatcccct tggacccgcc ggtgacgatc gccacggaat
    47461 ctttgagaag cccatccgcc atgacgccct cctacaggga aatcgcggcc acctggtcca
    47521 ccgtcccgca ggggtggcag ggaaccgtct cggtcacgct tttccacagg ccgcccagga
    47581 ccgtgcccgg gcccgcttcc aggcatgcgt cgaagcccgc cgccaggatg gccttctctt
    47641 cctcgaccca tcgcacggcg ctgatgatct gctcggcggc gcagcgcttg gcttccgccc
    47701 cggaggtgat gatcccgccg gtcacgttgg agtagaaggg gatcttggcg tccttgaact
    47761 ccacgtccga gaggagttcc ttgaagccgt cgtaggcgta cttcatgatc ggggaatgga
    47821 aggccccgga caccttgagc cggatgaccc tcttggcccc cgccgccttc aaggggacct
    47881 cgatcttccc gagccccgat tcggtgccgg acacgacggt ctggctggaa gagttgaggt
    47941 tggcgggcca gacgtcgtcg atccccgcgc ccttgagggc ggcctcgatg gcctccgggg
    48001 ccagtccgag caccgcggcc atgccgctcc cgccggaccg ctcccccgcc tcggccatga
    48061 gccggccccg ctccgccacc aggcggaaca tgacgggcag gtccaggaag ccggcctcca
    48121 ccagggcggg ccactccccc agggagaagc ccgcggctcc cgaggccttc gtgccgtagt
    48181 cgcggatgca ggaagaggca gcggcttcca ccaccgccag ggctatctgg gtcacccgag
    48241 tctgcttgag gtcgtcctcg gtcccctcga atatgagctt cttcaagtcc agcttggaaa
    48301 tctccgacgc catgtcgaag atctccttgg ccgccgtgct ggtctcgtag aaatccttgc
    48361 ccatgccgat atactgcgcc ccctggccgg gaaacaggaa acatgccttc acgggatcct
    48421 ccttggcaaa gtgttgaaaa aggccaagga gcctttttca acactacctc aatctcgcaa
    48481 ttgcgcagag aaccgcaggt tctcgagcaa ttgcatggca ggttgttgaa atgcttcgag
    48541 tatttcaaca acctgccagg atcggactag gggaacgcta gcacgccccg aaaaggggtg
    48601 tcaaggatat gacactatga ctatgggtgt cattttgccc tctgacgaat tccagaattt
    48661 tccgacagga tggacaagat gaggaaagga tgaacaggat gaatcagaat gcgaaaggcg
    48721 ggtggacgac ttccctccct atatctcatc ctgtccatcc gtcataatcc agttcatcct
    48781 gtcggatcga tgatgcgaat gccctgcacg tcggcgccgg aagggagaaa accatgagtg
    48841 atatcggcga ccggatccga gccgtcctgc gggagatccc ggccggcagg gtcgtgtcct
    48901 acgcgggggc ggcggcccgg gcgggggtgg ccaacggggg ccggaccacg gcccggatcc
    48961 tccacgcctg ctcccggaag gacgggcttc cctggcaccg ggtgatccgg agcgacggcc
    49021 gcatcgccct acccccggga gggggcttcg agctccagaa gaccctcctg gaggccgagg
    49081 gggtggaggt cagccccgag ggtcgggtgg atatgaggcg tttcggctgg taaggggccg
    49141 tcaggagccg gaagaggaaa gccaacgggt cccgaaggag gcgtacccca agccctccag
    49201 ggaggcgccg aactccggcc acaggaaggg catgtccaca acgaagtgcc ggatcccctc
    49261 ccggaacgca tcctcccggg cccgctcgat cagggtccga cccaggccga tccggcggcg
    49321 ctcgggatcc acgtacagga agcccaggac cgcgaaggcc ccctccccgg accggaggat
    49381 acgcgtcagg gccatgcccg cccgggcacc gggctcgcac tccgcggagt agatggagta
    49441 atccgcccca tacggccccc ggatctcccg gtccagggtg gagaacacgt ggtcgaccag
    49501 ggtcccgggg aaccgggcca gggcgtcctc ccggccggcc cggaccaggg gctcgaaatc
    49561 cccccagacc tccgccccgt accggagtat ccggaagtcc gaaagcagga ggtcgtccgt
    49621 gtcctcgggc tcgagcccca ggcgctgaag cccccggacg tcccggagat cctcgtacca
    49681 ctggacgatc cgcgccttca tggcccgggc cttgtactcg taccacttcc gctccgcctc
    49741 gggatgggcg tccagggcgt ccttgaaggc gcggaaaacc ccccgtccgc gggaaagggc
    49801 ggccaggagg tccgcccgca ccaccggatg cccgatggtc tgggcgaact gctccatgag
    49861 gcggaacccg tcctggctgg tccaggtcgg gatcggttcg aggtccgggg cgtcctcgcc
    49921 cggctccccg gcctcagctc cttcgtcgat ccgggaggag aggattccgg tctccatgtc
    49981 gatgtaggac acggctccct ggttctccat cgcgaagatc acggattcca cgaccttgtc
    50041 gtccagctcg aacacgtccg cctccccggg gcttccagaa tagccgcggt ccggacgtcg
    50101 gtcaaggaaa ggcgacaggc gacaggaccc cgtattgcct ccgggagcgt tcggagctaa
    50161 agtagtcgcc ggtcccgacg ccggatgtcc gtcgacgggg gcaaggagga cgcatggaac
    50221 cccggggtgc cgacatcggc ttcatcggac tgggagtcat gggaatgagc atggcggggc
    50281 acctccgcaa ggcgggcgcc cggcttcacg tatatacccg gaccaaggaa aaggtcctcg
    50341 ccttcctgga agatggtgcc gaatggcacg actcccccgg ggccgtagcc gcggcgtccc
    50401 gggccgtgtt caccatggtg ggctaccccc aggacgttga ggaggtgtac ttcggcaagg
    50461 cgggcatcct ggagaccgcc cggcccggaa ccctggtcgt ggacaccacg acctcccgac
    50521 ccgacctggc ccggaaggtc tgggaggaag cccgcaagcg ggacttgcac gccctggacg
    50581 ccccggtgtc cggaggcgac ctcggggccc ggaacgcgac cctgagcatc atggtcggcg
    50641 gctcggaacc ggacttcgag gaggccaagc ccctcctcct gaccatgggc aagaccgtgg
    50701 tccgccaggg acccgcgggc tcgggccagt acaccaagat ggtcaaccag atcgtcatag
    50761 ccggcgccct cgcgggaacc gttgaggccc tcgtctacgc ggaaaaggcc ggcctggatc
    50821 cccggaccgt cctggactcc atctccaccg gggcggcggc atcctggcag ctctccaaca
    50881 tggtgccccg gatgctggac ggcaacttcg agccgggctt cttcaccaag cattacctca
    50941 aggacctgcg catagccctg gagtccgccc gggccatggg agcggacctc cccatgctga
    51001 ccctggcgga aagcctcttc gcgaagatgg tcgaccgggg gtacgcggaa aagggcaccc
    51061 aggccctcta cctcctgtac aagaacggga tcccgcggta gaacgcccgc gatgtacgaa
    51121 cgccccgtcc tggatcccct tcccgcgatc gtgtccgagg gccgcttcgc ctacgggacc
    51181 ttcgcgaagc ccttcgggcg gaccaacatg ctggacgtag agcggcccta ccatcacgcg
    51241 gtcccccgct tcataaagcg gttccgcctg aaggaatggg tggccttcta cctgggcagc
    51301 agccggtaca tcctgatgac ggccctgtac gacgccaaga gcgccgccct ggccctgttc
    51361 cagggctacg accgggaaac ccggaaaagc ttcgggttcc gccggatcct gccggggggc
    51421 ttcttccgct tcggcccctc cctggacggg tgcaaggtgg cgtacaaaag ccgccgggac
    51481 ctcctggcct acgacctgga cctgtccggg gggagccttc gggtccgggt ggaccaccgc
    51541 aggacccgga cctgcccggc cctggagggg gagttcaccc tgccctggaa cgaccgccag
    51601 acggccatgc agaccgtctg cctgcccctg ggcctcaacc gggccatgta ctcgaccaag
    51661 gccctctgcc ccatgggcgg agccctgttg atcggcgggg agaccgcgga cttctcgggt
    51721 cccgactcca tgggcatcct ggacgaccac aagggatact acccctaccg cctccggtac
    51781 gactgggtga tcggattcgg acaggacccc aagggacgcc gcgtgggctt caacctgacg
    51841 gacaacccgg tgaaggatcc cgagcgcaac agcgagaact gcatgtgggt cgggggccgg
    51901 accttccccc tgccgcccgt gaaggtcacc cgcccccggg gggtctacga ggactgggtg
    51961 atccaggaca ccgagggccg ggtggacctc accttccacc ccgagacccg gttcgacatc
    52021 cgcctcaacc tcctggccgc ccaggccgac tactcgggac ccttcggctc cttcaagggc
    52081 tttgtgcgtt ccggggaggg ggagaagatc gacgcgtcct gggtgtacgg gatcggggag
    52141 aagaagtatc tgag
//
LOCUS       DAOTEI010000002        47173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_545_length_47173_cov_6.002759, whole genome shotgun sequence.
ACCESSION   DAOTEI010000002 DAOTEI010000000
VERSION     DAOTEI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 47173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47173
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_545_length_47173_cov_6.002759"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="P5313_00270"
     CDS             complement(<1..501)
                     /locus_tag="P5313_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78823.1"
                     /translation="MKSRVLWTLAAVFLAAANLAGQEGAAVNGYARTKAGALLEDASL
                     FLSETTLDLRLSYRSGDAGFFANPVLYEREGQTEEPVLREAYMDFRGESFDLRLGKQQ
                     IIWGKGDGVFITDIVSPKDLSRFLVPDFEEIRGAVTGACLDLYLGSHALELVWLPAFT
                     PVTAPAA"
     gene            complement(501..1283)
                     /locus_tag="P5313_00275"
     CDS             complement(501..1283)
                     /locus_tag="P5313_00275"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRY78824.1"
                     /translation="MNAHANRVLILTATLFAAVALHAAEPSGTEIMQAVYDRPQGKDV
                     QGTLTMTLVDSKGKERVRSLRQVTGTFASGEKKLMVFLGPADVRGTSFMNWSYEEAGR
                     SDDQWIYLPALKRVKRISSEGRGDSFMGSDFTYDDLGDRHPSEDEHRVTGSESIAGED
                     CWIVESVPRDPKDLYSRTVTWVSKKTMVGVKREYYDHRGALLKTLTISKLESISGVWV
                     ILKTEMRNVQKNTSTRIEFSEVKVNSGIPEDEFTERAMTRGL"
     gene            complement(1280..3580)
                     /locus_tag="P5313_00280"
     CDS             complement(1280..3580)
                     /locus_tag="P5313_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRY78825.1"
                     /translation="MKPFYKWASRRPVAVFLVVLALVAASGILIRSRMRMETDLDEYM
                     PRDHPAFVFSDEAEERFLIRDAILVAVEHPESIFNPDTLEKLILIEEALAGFDEIEES
                     DIRSLHTAENILGTEDGLDVRDFYTDAPEDQAACDRIRDDVRANEMVRGRLVSSDERA
                     ALVLVDLADGEFSRDLYDKILALAEQFEGPETIHVAGRPIVEGTMAILGPKDMARMGP
                     LVILVIAATLFVVLRSLLRTLISLSVVLVSTVWTFGLMVLLGIPVYTVTIMIPVMLIA
                     IGVAYAIHIYNQIDYFTREHPGSTREEIAENAIDVIWNPVLFAGLTTMAGFVSLTTSQ
                     VYPVKYFGLFAAFGVLAALLLSMLLVPAGIVLFGAGTPRAKPTQSGSAAAGSGGFGAR
                     FADAILKRPKAVLGISLLVIVLAIFGITRVWINTSFLDNFERDSDIVRTDAFMNARFG
                     GTSTLNVILEARDADAFKDPALLRLMLEIQKSVKTRDKVGDSVSIADYLARMNKVMHE
                     DREEFDTVPESREMTAQYLLLYEMSGDPENLWKVVDNEYRSANMIVQLKSDDSRTTTN
                     VVSFLDGYADRLAEYGVTARYAGSGYKSLVFSGLILQGQISSLGLSVLIVFLLVSLMF
                     HSLLLGLVGTIPVVISIAVNFGSMGLLNLPLTTSTALISSIAVGIGVDYAIHFIERYR
                     ERMKEGHAAPEAGRFAMGLTGRAVFLNAAIVIAGFLVLLFSVFPPNRQVGALVSLNMV
                     TAFAGTVTIMFLVLRNFHTSIKEKNK"
     gene            complement(3671..4252)
                     /locus_tag="P5313_00285"
     CDS             complement(3671..4252)
                     /locus_tag="P5313_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY78826.1"
                     /translation="MSLSERQREILDAALERIDELGIQELTMKRIAATVGISEPALYR
                     HFTSKSEILSAVVGEMETVSAQSLESARRTGRGSAEVLASFLESHSRRFAEHPALTAV
                     LFSEDLFRNDPTLHSRVGAIVSNTQELLRSEIEKGRASGDFRRDLEPEAAALMLAGGF
                     RLLVSLWNLRGHNFDLIARTRSYLESAIKVLKG"
     gene            complement(4555..5433)
                     /gene="era"
                     /locus_tag="P5313_00290"
     CDS             complement(4555..5433)
                     /gene="era"
                     /locus_tag="P5313_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRY78827.1"
                     /translation="MQSKSAFVAVVGRPSSGKSTLMNALCGQKVSIVSPVPQTTKNTI
                     RGIVNRPEGQLVFLDTPGIHDSGQRLNQRLRDLAREALGEADLVLYVLDSTRAPGREE
                     QAIAALLAPYAAKAVAAVNKTDAESADPGRARAFLTESLPGLEALDISALTGTGTPEL
                     LARLYERAPEGPAWYPEEYYTDQEPVFRIAEMVREKVLLHTREELPHAVYVDYRDSGR
                     REDGALVYEADLVVERESQKGILIGAKGSTIRRIREEAERDLADIFPYAVVLSLRVRV
                     QPDWKKDEKLIRKLYY"
     gene            complement(5530..7035)
                     /locus_tag="P5313_00295"
     CDS             complement(5530..7035)
                     /locus_tag="P5313_00295"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRY78828.1"
                     /translation="MAQLCLCLTGSTLAENLRALDMYRGRIDLAELRADCLDPNEVFH
                     IRSFPELAGIPTILSVRRRADGGYFDDGEGARLVVFAKGLAFASRDRRLNFAYVDIEQ
                     DFRIPALEEAARTFGTRIIRSRYCPAGLPEDLDEIWTDLSRDPYEIPKLAAVLRGIGE
                     TRRLFEFADRLPDTEHIVSGMGEYGFCFRILTSRTGSLINYASPSLAGLPSAAPGQAT
                     AEEYENVYRWRKIRKDYSVFGILGGPSVLGSLSPSLHNAGYAELGMPAVYLPFPSDSL
                     EQFLALAEELGVRGFSVTVPFKEKILPYLTERAPEVSAIGACNTVVRTPSGWYGTNTD
                     AAGFERSVLDFLKRPDLKGVRTAIIGAGGSARAVAYALQKLGASACVVNRGMNKAKQL
                     AERHGFEWAGMNERAVELLGRYNDLIVQTTSVGMEGGVPGDPLEWYEFTGSEALVDII
                     YNPKETPVMRRAREAGCAVANGLGMLRAQGEVQFELFTGKKYPGLPFGDVV"
     gene            complement(7045..7569)
                     /locus_tag="P5313_00300"
     CDS             complement(7045..7569)
                     /locus_tag="P5313_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78829.1"
                     /translation="MKNRIPAAILLSLLVSAAAQVPAPRPDAPALPVPPAGTADRRAR
                     VLLPDDPAPLLDLSLEEAWNRLGPPRSILAVRGNEPWQDDVAFEYGDGLSVYWYRDRL
                     WQVRLSAGYAGSCFGFFLGDPADKALSLLGTPDRGESGILEWRLPWRGYPAKLRVVTR
                     DGAIAEVYVYRSDF"
     gene            complement(7621..8214)
                     /locus_tag="P5313_00305"
     CDS             complement(7621..8214)
                     /locus_tag="P5313_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRY78830.1"
                     /translation="MKNELATYILVALGLSMDAFAVSVSSGICIPRLEFRHGLRAAAF
                     FGLFQFLMPLAGYFVGAAFRGPIRGYDHWIAFGLLAFVGAKMIKEGFEVRTDAACDEE
                     ELRKRNILDLGTLVLLSVATSIDALAVGLSYSLLDQPILPPASIIGVLTFLVCLAGCE
                     FGKRLGAVFERWAEVAGGAVLVGIGIKILAEHLSNGL"
     gene            complement(8238..9206)
                     /locus_tag="P5313_00310"
     CDS             complement(8238..9206)
                     /locus_tag="P5313_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY78831.1"
                     /translation="MTDFWNDFPTVKKRLEAVSAEMASTVRSAGFPLAAELARVVESD
                     GKMLRPGFLLLAAEFGKPGRAADLAPLAASIELLHIATLIHDDILDEAPLRRGEPAFH
                     VRNGVKEAVLTGDWLFAQCFRLASAFSTPENARRFALVIGIVCSSEIRQDLDKFRYSK
                     SVREYLRKIAGKTAALFSLAFTLGAVEGGCDPGTVGRLRRTGYDIGMAFQVIDDILDY
                     ESTEGIFRKPVGKDISEGLCTLPLIHALERDDGRLAALLENPPFDRPAVDRIVRLIRE
                     SGALEASRGIARRYTERALREIEGLPDKPVRLTLRRVTERLLVRAY"
     gene            complement(9360..11360)
                     /locus_tag="P5313_00315"
     CDS             complement(9360..11360)
                     /locus_tag="P5313_00315"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY78832.1"
                     /translation="MADKKRILILGGGYAGVWAGKILEKKYRKRDDVEITLVDKKPFH
                     TLMTELHEVAGWRVEPESVQVPFRKIFGARKIDIRVDTITGVDFHAKVAKSSSAEYPF
                     DYIVIGVGAEPEYFGIPGVQENSFPLWSFDDAIRLRHHVEHMFAKAACETDPAKRRRM
                     LTFVVAGAGFTGIEMIGELLEYRQDMCRKHHLDPKETRVVNIEALPSILPILEEPLRV
                     RAENYMKKMGCEIFLNSAITGAEPGKVLLKDGTVVETDTFIWTCGVKGSSFTSVLDVP
                     KAERGRGRMQANDFMQSTAYPYVYLAGDSAFILEPNGKPMPQIVEASHCSAETVAHNI
                     MADIDGGEHHKFKPNYHGFMVSIGGKYGVANAGGMKTRGFMAMAMKHMINCWYLLNIA
                     GINQVWEYLKHEFLDMTTGRTFIGGFVSHRVRAYWPLLLRMWLGLMWVMEGVNKIGEG
                     WLKFDLGSKSGWMFSSGVVQKGVADAASAASGEWAADATAAASGAVEAAAEAVTAASG
                     EWAADATAAASGAAEAVAEGAGKALGKVWDLAKPIFDFNGPVSTWFRTTFMDGMFAHL
                     HFAAFQGMIVVMEILIGLALFGGCFTWLAAVASIGMCVVFTLSGMFAWDQLWFVFAAI
                     LCMGGVGRAFGMDYWVVPFFKRWWNGTKFARKNYFYADDPTK"
     gene            11564..12265
                     /locus_tag="P5313_00320"
     CDS             11564..12265
                     /locus_tag="P5313_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRY78833.1"
                     /translation="MSTEDTQHGFPAAEAEALIGKILEDVRSIRDPAILNACRSLFRS
                     RVPFHLRSYVAAALVLRALGASAGPRRSVPVPAPGPNRVVGPKGEKRSPGSAPGKPGA
                     PSGKAGTENALESADLRIRKGSYSGEGTTLFFSMGRRQRFTAQNVLRVLSGISGLEES
                     DIGSIRTFDNYTFVNVHPAAADLIIQDADGTSFKGRKLAVNRARPRKEESRPGRPEDR
                     PEGEEETEGDDPVNE"
     gene            complement(12255..13217)
                     /locus_tag="P5313_00325"
     CDS             complement(12255..13217)
                     /locus_tag="P5313_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78834.1"
                     /translation="MLRSLTLAVLAALLFYGVLPVAGAFSARAGWRTFRRRFLASLGY
                     APLALGDGLRSPEGEVLVRRHSGSAEVLQGEDRLWVRGDGTTAAVLLKDARIYLLPSA
                     RTPEGPYDLEAADESLQPAAWNRLSGLSEGARVYAAGNLRIEGGLPVFFGSPAEPLLI
                     LIHDGRDGDLARRAVWAGRHRNEYWNPVTLVSLTAGFLSTGAALYDLLRPPLLSLPAA
                     LAAALTVSPVLPFLPPGLAFLSIYRRLWARARYLRAHRDLLRSGLEDPGGKYGPGDAE
                     DPEDAAEARVRRARKDEVLAGLAFAAALSSNFLLAVLAVRGIIR"
     gene            13275..13898
                     /locus_tag="P5313_00330"
     CDS             13275..13898
                     /locus_tag="P5313_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY78835.1"
                     /translation="MSVGPIGVNVHIVDLGGGRAIVVDPGAEPERIAAALEGRKPVCV
                     ALTHGHLDHTAGLPGLLALLGPVPVAIHAADARYLGALGEETNLRTFTDIGARSFFTR
                     FWSPLPEADVLLSDGDSLPGTSIRVLHTPGHTSGSCCFYIEGDSALLAGDTLFRGGLG
                     RTDNFDSSEEAILDSIRRRILVLPPDTTIFPGHGETTTVAAERPYFL"
     gene            13969..14991
                     /locus_tag="P5313_00335"
     CDS             13969..14991
                     /locus_tag="P5313_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRY78836.1"
                     /translation="MRRIVPALLCLSLSLSACARTGTREPLRGSEYVLGTICTVSLQD
                     GGSEAVLQAVFARLREIEARMSANREGTEIAAVNEAAGRSPVRVSPDTLFVVKKALEY
                     ARLSNGAFDPTIGPVVKLWNIGLDGQRVPSDREIREALPLVSWKSVVLDERASTVFLP
                     RSGMRLDLGAIAKGYAADETARILGERRVRAAVVDLGGNVLVYGKKADGSLWRVGVQN
                     PFNDRGSYLGIVSMGAGTVVTSGVYERFFEQDGKRYHHILDTRTGSPVESEIVSVTVI
                     APSSIDADGLSTTLFALGRERGLALVRTLPGVEAIYVDDQRRLYLSPGASKAFNLTDK
                     TFTFAE"
     gene            complement(14975..15880)
                     /locus_tag="P5313_00340"
     CDS             complement(14975..15880)
                     /locus_tag="P5313_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRY78837.1"
                     /translation="MDEILAWGLEVVRSVQTAASPGLTASFRFLSLLGTEYFFLFALP
                     VVYWCVDHRKGIRLGLLVLVSAVLNSWFKVVFRQPRPYDLDPSVGMARETSYGLPSGH
                     SQSSATFWGVVAPWIRAPWGLVLALALPFLIGLSRVYLGVHFPTDVFLGWALGALFAA
                     SHAVWGDTLEALVGRLPGRLWIAAAAAAALGMNALNPEETGLPGALFGMVVGAVLAAS
                     RARFDAASGGLARKALRLAAGTAGAAVLYFGLKAVLPGEGSANYALFRFLRYGVLGAW
                     VSLGAPWVFLRLGLAEERPAATPRT"
     gene            complement(15959..17956)
                     /locus_tag="P5313_00345"
     CDS             complement(15959..17956)
                     /locus_tag="P5313_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY78838.1"
                     /translation="MESDSGRECSCATCSRAAEAESGARSRAGDPGRAVDPALTREGL
                     FLGAAALVAGAGFLLESGLTGGPDAKPLVLALFVAAYLLAGWNVIAGAVRNLLAGRPF
                     DELFLMTVSTIGAFLIGHPEEAVGVMVFYKIGELLQESAALRSRRSIRAVLALRPDSA
                     RVLRDGRPVVVRPEEVRVGEPVRVRPGERVPLDGVVEHGEGWVDSSALTGESVPRRAL
                     PGVEILAGSIAREGELTVRVIRPASESYAARIADLVENASHAKARPERFITRFARWYT
                     PAVVAAAAGLAFLPPLLVPGQSLSDWVYRALVMLVISCPCALVLSVPLGYFAGLGGAA
                     RRGILVKGSQAFEALAEARTVVFDKTGTLTRGEFEVRAVIPRDGRSEREVLEGAVRAE
                     SGSNHPIAAALRREAGRRGLPPAPEAGAPVYRRETAGRGVSARDGESHVLAGSAAFLA
                     EEGIALPEAPPEPELGAGTEVLVAADGSYLGRILLGDSVKPDAREALDRLRSQGIRRF
                     VLLTGDARGPAHEAASELGIGEVHFGLLPHEKLERMETLLAKRNGEPGGILFVGDGIN
                     DAPVLARADVGVAVGTGADAALETADAVLMSDRLSALPEAVFRARKTRGIVRQNIVFA
                     LGFKLVFLALGAAGFADMWLAVVADVGVALTAVLNSVRALK"
     gene            complement(17943..18329)
                     /locus_tag="P5313_00350"
     CDS             complement(17943..18329)
                     /locus_tag="P5313_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY78839.1"
                     /translation="MSDSDRAAVCGCRTVHEDRVERAREAQIPVARLLRMGELFKVFA
                     DPSRLRILGALASEELCVCDLASALDMSQSAVSHQLAVLKRSRLVRYRRDGKTVFYSL
                     DDDHVRQILAVASGHLAEEDGIHGIR"
     gene            complement(18581..19750)
                     /locus_tag="P5313_00355"
     CDS             complement(18581..19750)
                     /locus_tag="P5313_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRY78840.1"
                     /translation="MQNVRRLELGGRTFVLVGTAHVSRESIDEAAAIIESEKPGRVCV
                     ELDEGRYRSMTKAASWESLDIGKVLREGKGFLLLANLALASFQRRLGGDIGVKPGAEM
                     LAAVETADRLGIPWSLCDREVQTTLRRAWGRSGLWGKSKLLASLLSGVFTNEKLSSEE
                     VEKLKERDELDGMMRELADYMPSVKEVLIDERDRYLAARIFAQPESSLVAVVGAGHLD
                     GLEAWLRKFTEGRSGVDVSDLEVVPPPSPLARALGWIIPLALVGLIAAGFFRSGAAVS
                     LRMLLQWVLWNGSLAALGSALCMAHPLTILASFLSAPAATLNPLIGVGMFAGLVEAFL
                     RKPSVRDFETLNEDISTLKGFYRNRVTHILLIFFLSSLGGALGNFISIPVLAGGL"
     gene            complement(19830..21260)
                     /locus_tag="P5313_00360"
     CDS             complement(19830..21260)
                     /locus_tag="P5313_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY78841.1"
                     /translation="MRGNRIFPAAFRTAILLAAILLAASCVGGPERAPVPEKVPDSAL
                     SPREREIRSMESLSDLLSKGDFTAAESRLRALIAEDPGKNEYRILLSSVLVSAGKVPE
                     ARAAAAEAVSAAPADPGALMAVVQVERYSGDDKALRAALDKVLAVDPANAEALAAYGD
                     SWYAGKNYTKAEELYRKSVASDPQNLSGLLGLGRTLLRRDKPADAELVLNQAVQSHPA
                     SSQARADRSRVLYRRGKYAESLSDLDEAVRLDPAVAWYRIERGRFRMDMGRLDEARAD
                     FDEAIRLDPDYFLPYVYRAGMLEQAGRDEESRADYRRITALYPDYWYAFESIGVTSFR
                     LGDFPAAREGFEKALSYSPGRMEYGILAVISAYRAGQDAEGKALAARYLARVSRETQN
                     LHWLILRLLQDRNDAVSELETKIPAERSLDTRAEMLFYLGQYWMIQGKASLGARYLLL
                     AKDMGREGILEHRLAEKELERISPNP"
     gene            21421..23232
                     /locus_tag="P5313_00365"
     CDS             21421..23232
                     /locus_tag="P5313_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78842.1"
                     /translation="MKLGAFGRGLALAVLYIGILLGLVLLQFREGGDFTRVSSGITLK
                     GRAGEQEGSLVSGTLSFGGLVLVFSPAKPLAWTAQDGGSRSALPKSFQETKDGFLIQF
                     TDGLELRARSSGEPGSRASFEFSRRTGPLKDVTVSVAAARRFRLVQDSRTYLVSGSAG
                     RYVLEVPEGTVSPDRKSLSLPFTRGRVAPVALSKAPDEAQTASPRFLEQAALDPEVFQ
                     REISAYRDKVWKGLSSDRFLPGTVEWRDAAGKAAFSEPALTAYLAEAWSRGQGPSAFE
                     RVQAANTLHASSLTHLSVPFLGRTTDRMRAFEEADLLEVKRIERLVQAKNPSLLEKPG
                     LVRFLLDRAPYALARDAFQMAGALDLGSLTVAQAAGGLAAWAESEDLFPAGENPFARF
                     AGIPERQILPAVVRAGDGYFLSTGPDGSSHLETSIRAGLSLIRLGEPTAKPMFTGIGQ
                     SLVLGALRLADPLGFLPRTVVVRNGAVADRLEILSPEEAYPLLSGNLYYPREVSFFKE
                     VGPGAWMWTCSPAVSISATPERMVWTLEFPEQGVHYIAAYGVKPFAKMQLYGLDYPMD
                     PAFENYNASGYLYRRASNVVYFKMRHKSRTEDIRMFY"
     gene            complement(23264..23575)
                     /locus_tag="P5313_00370"
     CDS             complement(23264..23575)
                     /locus_tag="P5313_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78843.1"
                     /translation="MKKYALLTLALGVLLTGCATVWKTLGVATVQSVEENNAQIARRI
                     DELAVRAASGDAALAKAVELEKLVKELQGRMDGLPADTLARLADLLAKAAEEARKAAG
                     K"
     gene            23786..24163
                     /locus_tag="P5313_00375"
     CDS             23786..24163
                     /locus_tag="P5313_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY78844.1"
                     /translation="MRRSILLFIALASAAAAVQAQPKPISPRAARDLLSADKNALLLD
                     VRTREEFLEVRIPGSVLLPFDAIDAASAARVVGPKDRTVVVYCRTGRRSAVAASTLAS
                     LGYRNVHDMGGIGSWPFGTVRGE"
     gene            complement(24260..25543)
                     /locus_tag="P5313_00380"
     CDS             complement(24260..25543)
                     /locus_tag="P5313_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY78845.1"
                     /translation="MPVEVILLGSFALLILLGIPISFSLGISSLLAATAVGMGPAVIA
                     QRVAASMQSFPLLAIPLFILAGSIMARGGVAKRMVDFAYILVGPLPGGLAIVNCIESM
                     FFGGVSGSAVADVSSTGSIMIPMMVEKGYDRDFSVAVTVASASQGIIIPPSHNMVIYA
                     MVAGGVSVGRLFLAGYIPGILLGLALSAACFIIALKRKYPCEGRPPLKRSLRIAADGI
                     LALLAALIIVVGIAAGIFTATEASAVSVVYAGFLGFVVYRELRIRDLWPILMESVKTT
                     AIVLFLIGCASAFGWMMTYLQIPAAVMNAFLSLTKNPFLILLLVNILILLLGMVMDVA
                     PLIVIVTPVLLPVVKAARMSEVTFGILLMLNLGIGLTTPPVGASLFVGCIVGKTTIER
                     TSRALLPLWPAMIVVLLLITYVPWFTEFLPGLFMK"
     gene            complement(25533..26051)
                     /locus_tag="P5313_00385"
     CDS             complement(25533..26051)
                     /locus_tag="P5313_00385"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY78846.1"
                     /translation="MPDLFSRILDRLERVLTGIAAAIMGVLALIVCWQVFARYVLQKS
                     PVWVEEFSVTAIMWIGLLGAAAAVWTGDHMRLEWVTKRLPKRYGLALEILIDAAIVYF
                     AAFLLSNGRLLTEATWTSRMSTIPLPLGLTYLVLPIAAAFMIAFAALRILRKAAEALE
                     SRSPRKEGDHAR"
     gene            complement(26169..27140)
                     /locus_tag="P5313_00390"
     CDS             complement(26169..27140)
                     /locus_tag="P5313_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY78847.1"
                     /translation="MKRILACVLLTALALTLATAAPKYNWKLASVLPDTHPVHQALVF
                     FADELARKSGGDIKLTLFPAGQLGQEKDYIESIKIGSLEFAKVSSGPVGQYAPTFQVI
                     SLPLIWRNLDHQHKVLQGPIGKRLMADLDKAGYKGLAFMDAGFRSITTSKGPINTPAD
                     LKGLKIRVMQSKPLIDTVNALGGNAVPMGQSEVYSALQTKVIDGWENNEPTVLSFNMQ
                     EVAKYYSYTRHSSIPDFLMMNKKLFDGLPKNLQTMIEQTALEAMEKHNKLWGEMIDKT
                     VADLKAKGMIFNEVKDISEFQKAVGGVWKEFEPIVGKDLIDAIVTAK"
     gene            27316..28077
                     /locus_tag="P5313_00395"
     CDS             27316..28077
                     /locus_tag="P5313_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY78848.1"
                     /translation="MDRLRGKKALVTGGGSGIGNAIVRELADRGAEVLIHYFKSGDSA
                     RALVDTIRANGGAAEAVQADLSAEDGARSLAGFVERRWGSLDVLVNNTGDLVGRRRLE
                     EADLAFYRSVMAVNLDSLVFVTRALIPLLRKPGAASIVNLSSLAGRKGGHGGSLVYST
                     AKGAILSFTRSLSTELAPDGIRANAVAPGLILGSAFHRIHTTEESKQATIRDIPLRRA
                     GTCEDVARAVAFLAGEYDGFITGATLDINGGVYMA"
     gene            complement(28291..30066)
                     /locus_tag="P5313_00400"
     CDS             complement(28291..30066)
                     /locus_tag="P5313_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRY78849.1"
                     /translation="MDGRDVAVTINGVRVRVPEGTMILQAARTAGYEIPSLCFHPDLR
                     PNGTCGMCVVEIEGVGVRRSCCTPAGDGMDIRTNTPRVMDTRRTLIELILADHAADCP
                     ICDKHGKCDLQRIGQDLGIREIRMAPKEVFSRRSDGGIVLRDPNKCILCNRCVTVCTE
                     VQGVANLTRIGRGYTSKIGTAFDLPIEDSVCVNCGQCVVYCPVGALSSRDDTREVRRS
                     LADPGKHVVVQEAPAIRAALGEEFGLSPGAVVSGKMHAALRKLGFDRVFDTNFAADVC
                     ILEEGTEVVRRILSGSPDLPVITSCSPGWIKFMETFYPGLRSLVSSAKSPQQMFGALA
                     KTYYPREAGLKPEDIVSVSVMPCTAKKFEADRPEMRSSGFKDVDYVLTTRELARMIKE
                     SGLRFEDLADAPADELMGSYTGAATIFGATGGVMEAALRTAYHLVTGRELENVDILPV
                     RGMEGVKEAVVPLPLPDGGTLDLKVAVAHGLGNARRILDVIRNQLETTGRSEYHFVEI
                     MACPGGCVGGGGQPFGSTLGTRAERGSGLYEEDRTLPSRKSHENPAVQRLYETFLGSP
                     CSEKAHHLLHTAYVERERVHGRAAG"
     gene            complement(30042..31709)
                     /gene="nuoF"
                     /locus_tag="P5313_00405"
     CDS             complement(30042..31709)
                     /gene="nuoF"
                     /locus_tag="P5313_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRY78850.1"
                     /translation="MIPLMHVILDVGAEAEQAGARKFREVLEAKLRKEFLSEDVRIVE
                     SANLGVSGQGVCMLVYPDEVYYGNLKLSDADRIVDQHFINGKPVEELFLDSPEMRRLM
                     AWRRREGIIKKGRGRVVLDNVGAVDPENLAEYKSRDGFRALAEAVRGGTPEGVLDTVK
                     RSGLRGRGGAGFPTGMKWEAVREAPGDRKYVICNADEGEPGTFKDRIILEGDPFRVLE
                     AMALAGYATGAEEGYVYVRGEYRTSIDRLENAIRKSREAGYLGRNILGSPFSFEVRVK
                     EGAGAYICGEETALIESLEGKRGTPRLKPPYPVTEGLWGRPTVVNNVETLANVPPILR
                     NGADWFRKFGTERSPGTKIYLIIGHVVSPGLVEVETTTSLRELIYGYGGGIRGRRRLK
                     AVQVGGSSGGIFDPSVLDEAMHFDALKEKGGILGSGSMLVMDETTCIVDMVHGILNFF
                     LHESCGACFPCRIGNERLVRTVNGFRSGSARPSDIDLLEEIASAMKVASYCALGQSPF
                     LPLSTAIRYFREEFEEHVNGKCRAGVCRFKVLKERQEARRWTVVMSR"
     gene            complement(31706..32167)
                     /locus_tag="P5313_00410"
     CDS             complement(31706..32167)
                     /locus_tag="P5313_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY78851.1"
                     /translation="MTTSQIIRSLEPTQDNLLAILHRVQDANPANFLDQPELREVADY
                     LELPYSFVYGVATFYSMFSVKPRGRHVVRVCASPPCHLMGGRTIRDELREYLGVEFGD
                     TTEDGLFTLELSSCLGTCGIAPAMMIDGEIFGNLDGVRLREILDRKRREEP"
     gene            complement(32374..33312)
                     /locus_tag="P5313_00415"
     CDS             complement(32374..33312)
                     /locus_tag="P5313_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78852.1"
                     /translation="MTRDRAFLKGLRNTGGFLATLLLAGAAFIAASLAVPVPGAIRAA
                     VPASGFLPAPLDFLSFLGSLALLAYWTARRSAVPGWKRVGQVAVLVIGAQTFMTQIET
                     AYFLEAFPLLQGSFETYRMVLRGALWGFAAAAAATLLTGGFRKTAELREPRFTVDPVR
                     ALARTAILGAAYLVLYMLFGYYVAWQSEAVRLFYSGNADKLGFFAQWGRSFMEKPEIP
                     VFQWFRGCLWVLCLVPLFRGFTGGRVELTILSGLALGILPTAQLAFPNPLMPPAVSLA
                     HFWEVSVSTGLLGALAALWVPEPAKPAGKPEVRIDR"
     gene            complement(33309..33917)
                     /locus_tag="P5313_00420"
     CDS             complement(33309..33917)
                     /locus_tag="P5313_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY78853.1"
                     /translation="MKPFLEEERSRIRNALRETAAVEFGRRGIRGTGIQELCGRAGIA
                     SGSFYAFYDSKERLYAELLRERYAANAEAFRRVSAMTSRPAETLEALMKGALEAFRSD
                     PLLRRMTDPEDRALLRRKLPEEVWKALDEASLGAMVTFVRDWQARGLLVEGDPRTLAL
                     LLTSHFFLYLHADQIGEDRFDAVIALFARCTARGLTDVRRVP"
     gene            34186..34863
                     /locus_tag="P5313_00425"
     CDS             34186..34863
                     /locus_tag="P5313_00425"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HRY78854.1"
                     /translation="MALPLLQLALDNKTLPDALVSTRLLASEVDVLEAGTILCCSEGM
                     QAVRCLKALYPNRIVLADIKAADAGTILANMVYDAGADWMTVICCAPIATVETALAAA
                     KARNEAGKTGGVPRDVQVELTGSWTWEEAAAWRAAGVKQVVYHRGRDAQAAGQSWGEE
                     DLAKIRRLADMGFDLSITGGLVEEDLPLFKGIPVKVFISGRSIRDAADPAAAARSFKA
                     RIAELWG"
     gene            34878..35738
                     /locus_tag="P5313_00430"
     CDS             34878..35738
                     /locus_tag="P5313_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HRY78855.1"
                     /translation="MPLPPRIGIYEKALPAGLDWPGMLRAAREAGYDYVEMSVDETDA
                     RLARLEWSAGERAAFREAVADSGLRVPSMCLSGHRLYPFGSADPAVRERSRDIMVRAL
                     DLAADVGIRTIQLAGYDVYYEPSTPESRERYREGLAEAVELAERAQVCVAMEIMDTPF
                     MGTVSRWLEFAEKLPSAWFQVYPDIGNLSAWAEDVPGELAKAKGRIAAVHLKDTLKPR
                     PGFPGRFREVPFGTGCVDFPGAFRALRSIGYRGSYLIEMWTHQSPDPAAEIRAAREWI
                     LGRMREGGVA"
     gene            35735..36430
                     /locus_tag="P5313_00435"
     CDS             35735..36430
                     /locus_tag="P5313_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRY78856.1"
                     /translation="MTVEELKEAVFRANLELRDRGLAILTWGNASGIDRERGIVAIKP
                     SGVPYDVMKAADMVLVDLDGKVLEGKLRPSSDLATHLVLYRAFPSLGGVVHTHSVHAT
                     ALAQAGADLAAEGTTHADHFYGPVPCTRAMTRAEIEGEYEAETGNVIVETFRTRGIDP
                     ARVPAVLVRSHGPFAWGKSPGDAAMNAVVLEEVARMSILSRSFGDPGPMPKELLDRHY
                     LRKHGAGAYYGQK"
     gene            36550..37176
                     /locus_tag="P5313_00440"
     CDS             36550..37176
                     /locus_tag="P5313_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HRY78857.1"
                     /translation="MSDPLNQPHSKTSIVVANPSAMGLFGLAMVTLVASTQKLGWTSG
                     TAFELPWALFLGASLQLYASILDAKLNNAFGATAFGGYAFFWFAVGMTWMIQGGWFGA
                     EVAARADVRQLGVAFLGYLIFSLYMTLGALATTKVLFGIFVLIDLLFLGLSLSVLGIA
                     REFFHALAGWAELAIALASFYASAAAILNAQFGRTVLPLGKAFGIRRG"
     gene            complement(37355..38419)
                     /gene="ulaG"
                     /locus_tag="P5313_00445"
     CDS             complement(37355..38419)
                     /gene="ulaG"
                     /locus_tag="P5313_00445"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HRY78858.1"
                     /translation="MAKIDGITRESWILGTFPEWGTWLNEEIEEEKVAPGTFAMWWLG
                     CTGIWLKSPGGANLCTDFWVGTGKKTHKNPVMAAGHQMQRMTGAKKLQLNLRNSIFPL
                     DPFAIRNVDAILATHDHNDHIDVNVAAAVLKNCDPAVPFVGPAACVKLWTSWGVPETR
                     CVTVKPGDTVKVKDVEILALESFDRTALITAPADQGLSGRMPQDMDERAVNYLFKTPG
                     GSLYHSGDSHYSNHYAKHGNEHRIDVALGSFGENPRGVTDKMTSVDILRMAECLNAKT
                     VIPFHHDIWTNFQADPMEVVALWRMRKDRLGYGFTPFVWQVGGKFTWPADRDRIEYHY
                     PRGFEDVFEGPTDLPYPSFL"
     gene            complement(38504..39463)
                     /locus_tag="P5313_00450"
     CDS             complement(38504..39463)
                     /locus_tag="P5313_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY78859.1"
                     /translation="MNKKPIATELRLAVVLVPLMAVLSVLSPRFLTLDNLTNVVWSVC
                     LIGILSSGAIYPRITGGIDLSLGSTAALSGIVVDLFMNRMGIPWIPSILMTVVIGALV
                     GLFNGFIVAKIKIPAFVVTMAAKTYLYGIALVVSQGAMLAILEPRPFILISTGRFLGL
                     PNPIYIMVALAVTSQFVLRRTAFGRQVMAVGSNEVAARLSGVDPDRVRLISYTISGGT
                     SAVGGVILASLTQQVFAAAASGIELDVMTALVIGGTSAVGGRGSVLGALIGAIMVAFI
                     INGLNLMNIPAPFHPIVTGSVIILALLLNEGYLVPKRRTLSRA"
     gene            complement(39456..40988)
                     /locus_tag="P5313_00455"
     CDS             complement(39456..40988)
                     /locus_tag="P5313_00455"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY78860.1"
                     /translation="MTKVENGADLLLECRSITKSYSGHVVLDAVDFSVRRGEVHALVG
                     ENGAGKSTLIKIVTGATGRDSGEMVYDGTVVPMEHLRRDAEALGIAVIYQELSLIPGM
                     TVAQNIFLGKEPLRPLTRLIDVRKMNDQARELIDRYNFPLKPTDLIDNISIAQRQLVE
                     ILKALSGKASIMIMDEPTSSLTKTEVEMLFKIIALLKSEGISVLYISHRMEEVYALSD
                     RVTVLRDGKLVKVLDKEEISPEEIIRLMIGHKLEEKAVQHELTPKGGKVVLEVRGLTS
                     VGKFEDLSFDLHEGEVLGIGGLVGSGRTELVRALYGADSFDAGTIRYLGKEWVPSVRK
                     SIEGGIGFIPEERRLQGILATQSITRNIGIPNLDLIAPRGIVSVKEEDRRAARGIELL
                     NVKPPRPEQLVGNLSGGNQQKVVLAKWLVRDLKLIFIDEPTVGIDIGAKEEIYDHIRA
                     LSARGVSVLLVSSDLPELTRLSDRVIVLRQGRFFQEFRECVLTGEKILRAASGIREGD
                     DE"
     gene            complement(40998..42011)
                     /locus_tag="P5313_00460"
     CDS             complement(40998..42011)
                     /locus_tag="P5313_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY78861.1"
                     /translation="MSHHAVRRLILVGLMLALSALITVINPVFLSSGNLLKLLQEAAQ
                     TGIVAVGFTFVMITAGIDMSIGGVVAVTSMVCINFLHYTKVPIPVLVFLSLFVGALTG
                     LVNGFFITKYKLPEFIVTLATRSILAGLALVIAVKDSLGFVQNVYIQNDSYLWFGTSG
                     LFGIYRTTVAFLILGVGAQIFLKHTRTGTNIYATGANPTAARLTGIDTDRTTIGVYMF
                     SGICASLAAVFISSRMMTAMPELGLGSEMDVIASVVIGGTAFSGGVGDVWGTMLGSLF
                     LALIKNGILKTGISPWIQPIAIGGIIVVTVIVDVWQKKLREHLEARAKLKRLSAERGR
                     DAA"
     gene            complement(42112..43056)
                     /locus_tag="P5313_00465"
     CDS             complement(42112..43056)
                     /locus_tag="P5313_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY78862.1"
                     /translation="MKKTAIVAALLCVLLVVPAVAQTYKVAYIPQSIGQPNTNAWLEG
                     MMRVFRKYPNVKVQSFDAQLKAELQVSYMDDVINQKYDAIVLQAMDAAALSASVKAAE
                     AKGIPVITVNLATIQKHTASVQMADISAGYAVGKEMGKQLGGKGNVVIIQSPPGAILG
                     VNREKGFRQAMAELYPNIKIVGAQSAEGWKKEIAITIMNGFLQANKELAGVFAVNDNM
                     AEGAMIAAEAAGRLDQIKIWGANGQKSTLALIEQGKIAGTAYNNSFAQGSLIAEMVME
                     NLQKKLGPSKAAETKVIQIPPFAATKETVGQIAAEDRW"
     gene            complement(43272..44048)
                     /locus_tag="P5313_00470"
     CDS             complement(43272..44048)
                     /locus_tag="P5313_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRY78863.1"
                     /translation="MASNAAVYRRRLHILESVRSRGLATVEDLSESLGVSPITIRRDL
                     DHLCREGSLIRTHGGAVPQEESGSIPEKNLSEKDVLNIEAKNRIAAAAVRLLEDDEIV
                     FMNSGSTTLCFLRALAGRRAKVITNNVASLGVPASPGVELMILGGEYREQSQSLVGEF
                     ALSAIRDIWADHTFLGTNGLSLDRGLTTSVYQECSVNQAMIDHTHGKVVVLADSSKMG
                     KVANFVSSPLSNVDIVVTDSECPESFRKELEERGLQVILA"
     gene            44206..45702
                     /locus_tag="P5313_00475"
     CDS             44206..45702
                     /locus_tag="P5313_00475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRY78864.1"
                     /translation="MDTEGIIAIDAGTTSIRAVLFDRAGRPGAFAQAENPPVYFEDGR
                     VEQEASTWREALFRILSEVSAAAGSYGIRVEAVTITANRSCVIPADSEGRALHPALMW
                     QDVRTDGICRDLAPRSREVYERSGLPITSIFSAVKMAWIRRECPELWVRTARMLGVQD
                     YLVFLLTGNFVTNRSLASRTNLFNLESGDWDPELLGIFGIERRVLCDLVDPGETAGSL
                     APAAAAASGLSAGIPVITAGGDQQCAALGMGLLAAGDVVANTGTGAYVIGLADRPVRD
                     PDMRLYCNVSALPGRYVAEAASPAAGVAYRWFRDRFYGGPDGSADGFGTINAEVEASP
                     PGANGVVHLPRFKSMGPVRGIGTGGPGGRASFHGIGLATARGDLARAVLEGVAGDLAE
                     NLELIERWTGPKDRVRAAGGLTRFPEFDRILAGLCGRPVVLAAESEATARGAWISAAV
                     RLGLYPDAASAFAASGAGAGDRVFEPDPARREAYTRLREERKRMEVNE"
     gene            complement(45814..46788)
                     /locus_tag="P5313_00480"
     CDS             complement(45814..46788)
                     /locus_tag="P5313_00480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRY78865.1"
                     /translation="MAKPKVYVTRRIPDAGLDLLRKECEVEVSPHDRPLTRPEFLDAI
                     KGRDGVICLLTDKVDAEAFEAAAGVKGFANFAVGFDNLDVPEATRRRIPLSNTPDVLT
                     DATADMAWALLFAAARRVVESDGVNRSGAWKGWGPMQFIGGDVTGATLGIVGAGRIGA
                     AMAAKSVGFRMKVLYTDARRNEALEKAIGARFVSFDELLAQSDFVSVHVPLMPETKHL
                     FTYDTFKKMKRSAYLVNTARGPVLAEDDLVRALEDGLIAGAGLDVYEREPALAEGLAA
                     LPNVVITPHTASATVSSRNGMALKAATNVIAMVKNQKAPDCLNPEVYK"
     gene            complement(46871..>47173)
                     /locus_tag="P5313_00485"
     CDS             complement(46871..>47173)
                     /locus_tag="P5313_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78866.1"
                     /translation="INDNDGKWDWDYFCGTKHYLEYVEFLWYLRRIGYDGWFTSDTSP
                     TRWDVAGTFEANARLSSRIEKLLDRLDSKAIESRMGGPGYLETWKLLETEVFGLTD"
BASE COUNT         7741 a        15686 c        15865 g         7881 t
ORIGIN      
        1 ggcggcggga gcggtcaccg gggtgaaagc gggcagccat acgagttcca gggcatgaga
       61 cccgaggtag aggtccaggc acgcccccgt cacggcgccc cggatctcct cgaaatcggg
      121 gaccaggaac cgggacaggt ccttgggaga cacgatgtcg gttatgaaca ctccgtcccc
      181 cttgccccag atgatctgct gtttgcccag acggaggtcg aaggactccc cccggaaatc
      241 catgtaggct tcccgaagga ccggctcttc cgtctggccc tcccgttcgt acagcacggg
      301 gttcgcgaag aatccggcgt ccccgctccg gtaggacagg cggaggtcca gggtcgtctc
      361 cgagaggaag agggaggcgt cctccaggag ggcacccgcc ttggtccgcg cgtatccgtt
      421 taccgcggcg ccctcctgtc ccgcaaggtt cgccgccgcg aggaagacgg ctgcaagggt
      481 ccatagtaca cggcttttca tcatagcccc cgggtcatgg cccgctcggt gaactcgtcc
      541 tccgggatcc cggagttcac cttgacctcc gagaattcga tccgggtgct cgtgttcttc
      601 tggacgttcc gcatttccgt cttcaggatc acccagacgc cggaaatgga ttccagcttg
      661 gagatcgtca gcgtcttgag aagggcgccg cggtggtcgt aatactcccg tttcactccg
      721 accatggtct tcttggacac ccaggtcacg gtgcgggagt acaggtcctt agggtcccgg
      781 gggacgcttt ccacgatcca gcagtcctct cccgcgatcg attcgcttcc ggtcacccgg
      841 tgttcgtcct cggagggatg ccggtcgccc agatcgtcgt aggtgaagtc ggagcccatg
      901 aaggaatccc cccgtccctc ggaggagatc cgtttgaccc gtttgagggc gggcaggtag
      961 atccactggt cgtcgctccg gccggcctct tcgtagctcc agttcatgaa ggacgtcccc
     1021 cggacgtccg cggggcccag gaagaccatg agcttctttt ccccggaggc gaaggtgccc
     1081 gtgacctgcc ggagggagcg gacccgctcc tttcccttgg aatccacgag ggtcatcgtg
     1141 agggttccct ggacatcctt tccttgggga cggtcgtaga cggcctgcat gatctccgtt
     1201 cccgagggtt cggcggcgtg cagggcaacg gcggcgaaga gggtcgcggt caggatcagg
     1261 acacggtttg cgtgggcgtt catttgttct tctccttgat cgaggtatgg aagttcctca
     1321 gcacgaggaa catgatcgtc acggttcccg cgaaggccgt gaccatgttg agcgaaacca
     1381 gggcgccgac ctgccggttc ggcgggaaga ccgagaacag gagcaccagg aaccccgcga
     1441 tgacgatcgc cgcgttgagg aacacggctc taccggtgag gcccatcgcg aagcggccgg
     1501 cctccggggc ggcatggccc tccttcatcc gttcccggta gcgttcgatg aagtggatgg
     1561 cgtaatccac cccgatgccg acggcgatgc tggagatgag ggccgtcgag gtcgtgaggg
     1621 gcaggttcaa cagtcccatg ctcccgaaat tgacggctat ggaaatcacc accggtatcg
     1681 tgccgacaag gccgagcagc aggctgtgga acatgaggct cacgagcagg aagacgatga
     1741 ggaccgagag ccccaggctg ctgatctgcc cctggaggat gaggccggaa aacacgaggc
     1801 ttttgtagcc cgatccggcg taccgcgccg tgacgccgta ctcggcgaga cggtccgcgt
     1861 acccgtcgag gaaggaaacg acgttcgttg tggtccggga gtcgtcgctc ttcagctgta
     1921 cgatcatgtt ggcggatctg tactcgttgt ccaccacctt ccagagattt tccggatccc
     1981 cggacatttc gtagagaagc aggtactggg ccgtcatctc gcggctctcc ggcacggtgt
     2041 cgaattcttc ccgatcctcg tgcatcacct tgttcatgcg cgccaggtaa tccgcgatgg
     2101 ataccgagtc gccgaccttg tcccgggttt tgacgctctt ctggatttcc agcatgagcc
     2161 ggaggagggc gggatccttg aaggcgtcgg cgtcccgggc ttccaggatg acgtttagcg
     2221 tggaggttcc gccgaaccgg gcgttcatga aggcgtcggt ccgtacgatg tcgctgtccc
     2281 gctcgaaatt gtccaggaaa ctggtgttga tccatacccg ggtaatgccg aagatcgcga
     2341 ggacgatcac gagaaggctg attccgagca cggccttcgg ccgcttaagg atggcgtccg
     2401 cgaaccgggc gccgaacccg ccggagcccg cggccgccga cccgctctgg gtcggctttg
     2461 cccggggcgt tcccgctccg aacaggacga tgcccgccgg aaccaggagc atggatagga
     2521 gcagggccgc gagcacgccg aaggcggcga acaggccgaa atacttgacc ggatacacct
     2581 gggaggtggt cagggagacg aagcctgcca tcgtggtgag tcccgcgaag aggacaggat
     2641 tccagatgac gtcgatcgcg ttctcggcga tttcctcccg ggtcgagccg ggatgctccc
     2701 gggtgaagta atcgatctgg ttgtagatat ggatggcgta ggcgaccccg atggcgatca
     2761 gcatcacggg aatcatgata gtgaccgtgt acacggggat gcccaggaga accatgaggc
     2821 cgaaggtcca aaccgtgctg accaggacga cggacaggga gatgagagtc cggagcaggc
     2881 ttcggagcac gacaaacagg gtcgcggcga tcaccaggat caccagggga cccatacggg
     2941 ccatgtcctt gggacccagg atggccatgg tcccctccac gatggggcgc cccgcgacgt
     3001 ggatggtctc cggtccctcg aactgttccg ccagggccag gatcttgtcg tagaggtccc
     3061 gcgagaattc cccgtccgcc aggtccacca ggacgagagc cgcgcgctcg tcggaggata
     3121 ccaggcggcc ccggaccatc tcgttggcgc gtacgtcgtc ccggatccgg tcgcaggcgg
     3181 cctggtcttc cggtgcgtca gtatagaaat cccggacgtc caggccgtcc tcggttccca
     3241 ggatgttctc cgccgtgtgg agggaacgga tgtccgactc ctcgatctcg tcaaacccgg
     3301 ccagggcttc ctcgatgagt atgagttttt ccagggtgtc gggattgaag atcgactccg
     3361 ggtgttcaac cgccaccagg atcgcgtccc ggatcaggaa ccgctcctcc gcctcgtcgc
     3421 tgaacacgaa ggccgggtgg tcccggggca tgtattcgtc cagatccgtc tccatgcgca
     3481 tccgggagcg gatcaggatg cccgatgcgg cgaccagggc cagcaccacc aggaagaccg
     3541 cgacgggacg ccgggaagcc catttgtaga acggtttcat ctgtgctcct tattgtagtg
     3601 cgagttatcg ataactcgct atttgggtaa acaagtcccg ccatgtcgcc gccctcggcg
     3661 attcccggcg tcagcctttc aagaccttta tcgccgattc taggtaggat ctcgttcgcg
     3721 cgatcaggtc gaaattgtgg cccctcagat tccagaggga gacgagaagc cggaatcccc
     3781 ccgccagcat caaggcggcg gcttccggtt cgaggtcgcg gcggaaatcc ccggaggccc
     3841 ggcccttctc gatttcactc cgcaggagtt cctgggtgtt cgagacgatg gctcccaccc
     3901 gggagtgcag ggtcgggtcg ttccggaaca ggtcttcgga aaacaggacc gccgtcaggg
     3961 cgggatgctc tgcgaagcgg cgggaatggg actcgaggaa ggacgcgagg acttcggcgg
     4021 atccccgtcc cgtccgccgg gcggattcga gggactgggc gctgacggtc tccatctcgc
     4081 ccacgacggc cgaaaggatc tccgacttgg aagtgaagtg gcgatacagg gccggttcgc
     4141 tgatccccac ggtagccgcg atccgcttca tcgtcagctc ctggatgccc aattcatcga
     4201 tccgttccag agcggcgtcc aggatctccc gttgacgttc gctgagcgac aaaaaggcct
     4261 cctttcttgg gggttagtta tcaatcacta atctacagga atcctttaca actgtcaatg
     4321 cttttttgaa gttcacggtc gtgatgcaag cccggggtcc tttgaatgcc accgaggaac
     4381 atgggcctgt ccaccaggcg ggattcttcg tcgcttcctt gactttcttg agatgtatcg
     4441 acgatgggca ggatcggatg atgagagaac cggatatctc cgaatctctt cctcccgtat
     4501 cctgtccatc catctcctat cctgttcatc ctgttgggat ccattctggc aagctcagta
     4561 atacagcttc cggatgagct tttcgtcctt cttccaatcc ggctggaccc gaacccgcaa
     4621 ggataggacc accgcgtacg ggaagatgtc cgccaggtcc cgctcggcct cctcccggat
     4681 gcgccggatc gtggagccct tggccccgat caggatgccc ttctggcttt cccgctccac
     4741 caccaggtcc gcctcgtaga ccagggcccc gtcctcccgg cggcccgagt cccggtagtc
     4801 cacgtagacc gcgtggggca gttcctcccg ggtatggagc aggaccttct cccgcaccat
     4861 ctccgcgatc cggaacacgg gttcctggtc cgtgtagtac tcctcgggat accaggccgg
     4921 gccttccggg gcccgctcgt agagccgggc caggagctcc ggagtgcccg tcccggtcag
     4981 ggcggagata tccagggctt ccaggccggg cagggattcc gtcaggaagg cgcgggccct
     5041 cccggggtcg gcgctttcgg cgtccgtctt gttaactgcg gccacggcct tggccgcgta
     5101 gggagccagg agggcggcta tggcctgctc ctcccggccc ggggcacggg tggagtccag
     5161 gacgtaaagg acgaggtccg cctcccccag ggcttcccgt gccaggtccc ggaggcgctg
     5221 gttcaggcgc tggcccgagt cgtggatccc cggggtgtcc aggaagacca gctggccctc
     5281 cgggcggttc acgatgcccc ggatggtgtt cttggtggtc tgggggaccg gggagacgat
     5341 ggacaccttc tggccgcaca gggcgttcat cagggtggat tttccgctgg aaggtcggcc
     5401 gaccacggcc acgaaggcgc tcttgctctg cataggtctc ctttgtttaa caatcgcgcg
     5461 gaaaagcgcg ggcttttcga gcaattgcag agcggggtgc tgacaagctt cgagcgtgtc
     5521 agcaccctgc tagactacgt ccccgaaggg caggccgggg tactttttgc cggtgaacag
     5581 ttcgaactgg acctccccct gggcgcgtag catccccagg ccgttggcca cggcgcagcc
     5641 cgcctcccgg gcccggcgca tcacgggggt ctccttgggg ttgtagatga tgtccaccag
     5701 ggcttccgag cccgtgaact cgtaccattc caggggatcc cccggaactc ccccctccat
     5761 gcccaccgag gtggtctgta cgatgagatc gttgtatcgg cccaggagct ccaccgcccg
     5821 ttcgttcatc cccgcccact cgaagccgtg gcgctcggcc agctgcttgg ccttgttcat
     5881 gccccggttg accacgcagg ccgaggcccc cagcttctgc agggcgtagg ccaccgcccg
     5941 ggcggagcct ccggccccga tgatggcggt ccggacgccc ttcaagtcgg ggcgcttgag
     6001 gaagtccagg acggagcgct cgaagcccgc ggcgtcggtg ttcgtgccgt accagcccga
     6061 gggggtccgg accacggtgt tgcaggcacc gatggcggag acctccggag cgcgttccgt
     6121 aaggtagggc aggatttttt ccttgaaggg aacggtgacg gagaaaccac gaacccccag
     6181 ttcctccgcc agggccagga actgctccag ggaatccgag gggaagggta ggtagaccgc
     6241 gggcatcccc agttccgcgt atcccgcgtt gtgcagggag ggggagaggg agcccaggac
     6301 cgaggggcct cccaggattc cgaagaccga gtagtccttc cggatcttcc gccagcggta
     6361 gacgttctcg tactcctccg cggtggcctg tccgggggcg gcggagggaa ggcccgccag
     6421 ggagggactg gcgtagttga tcaggcttcc cgtgcgggag gtcaggatgc ggaagcagaa
     6481 tccgtactcc cccatcccgg agacgatgtg ctccgtgtcg ggcagacggt ccgcgaactc
     6541 gaagagccgg cgggtctccc cgatgccccg caggacggcc gccagcttgg ggatctcgta
     6601 gggatcccgt gagaggtccg tccaaatctc gtccaggtcc tccggcagtc cggcggggca
     6661 gtaccgggac cggatgatcc gggtaccgaa ggtgcgcgcc gcctcctcca gggccgggat
     6721 ccggaagtcc tgctcgatgt ccacgtaggc gaagttcagt ctccggtccc ggctggcgaa
     6781 ggccaggccc ttggcgaaga ccacgagccg ggcgccttcc ccgtcgtcga agtagcctcc
     6841 gtccgcgcgg cgccggaccg agaggatggt cgggattccc gccagctccg ggaacgaacg
     6901 gatatggaag acctcgttgg gatccaggca gtccgcccgg agttcggcca ggtcgatccg
     6961 gccccggtac atgtccaggg cccggaggtt ttccgccagg gtgcttccgg tcaggcagag
     7021 gcagagctgg gccacgggca cctcctagaa gtccgagcgg tagacataca cctccgcgat
     7081 cgcgccgtcc cgggtcacca cgcgaagctt ggccgggtaa ccccgccagg gcagccgcca
     7141 ctccaggatg ccggactccc cacggtccgg ggtgcccagg agggacaggg ccttgtccgc
     7201 gggatcgccc aggaagaacc cgaaacagga acccgcgtac cccgcggaga gacgcacctg
     7261 ccacaggcgg tcccggtacc agtacaccga aagcccgtcc ccgtactcga aggcgacgtc
     7321 gtcctgccag ggctcgttcc cccggaccgc caggatactc cggggcggcc ccagccggtt
     7381 ccaggcctcc tccagggaca ggtccaggag gggggcggga tcgtcgggaa ggagcacgcg
     7441 ggcccgccgg tccgcagtcc ctgcgggagg caccgggagg gcgggcgcgt ccggccgcgg
     7501 tgccggaacc tgggcggccg ccgagaccag gagggacagg aggatcgccg cggggattcg
     7561 attcttcatc ggatcgacag tataccctcc gcggggcttc cttgtggagc ggcgaggagg
     7621 ctacagcccg ttcgagaggt gctccgccag gatcttgatc ccgatgccca cgaggacggc
     7681 tcccccggcc acctcggccc agcgctcgaa gacggcgccc aggcgcttgc cgaactcgca
     7741 gcccgccagg cataccagga aggtcaggac gccgatgatg gaggcggggg ggaggatggg
     7801 ctggtccagg aggctgtagg aaagccccac cgccaaggcg tcgatgctgg tggccacgga
     7861 cagcaggacc agggttccca ggtccaggat gttccgtttg cggagctcct cctcgtcgca
     7921 ggccgcgtcc gtgcggacct cgaagccctc cttgatcatc ttggccccca cgaaggccaa
     7981 caggccgaag gcgatccagt ggtcgtagcc ccggatcggg ccccggaagg cggcgccgac
     8041 gaagtacccg gccaggggca tcaggaactg gaaaaggccg aagaaggcgg ctgcacggag
     8101 cccgtgccgg aactcgagcc tggggatgca gatcccgctg gagacggaca ccgcgaaggc
     8161 gtccatggaa aggcccaggg cgaccaggat gtaggtggcc agttcgttct tcatggacgg
     8221 ctccgttccc gcgtccgtca gtaggcccgc accaggaggc gctcggtcac gcgcctcagg
     8281 gtcagccgta cgggcttgtc gggcaggccc tcgatctccc ggagggcccg ctccgtgtac
     8341 ctccgcgcga tcccccggga ggcctccagg gctcccgact cccggatgag ccggacgatg
     8401 cggtcgaccg cggggcggtc gaagggggga ttctccagca gggcggccag gcggccgtcg
     8461 tcccgctcca gggcgtggat gaggggcagg gtgcacaggc cctcggagat gtccttgccc
     8521 acgggcttgc ggaagatccc ctcggtgctc tcgtagtcca ggatgtcgtc gatcacctgg
     8581 aaggccatgc cgatgtcgta cccggttctc cgcagacggc ctaccgtccc ggggtcgcag
     8641 cccccctcca cggcccccag ggtgaaggcc agggagaaga gggccgcggt ctttccggcg
     8701 atcttgcgca ggtactcgcg gacactcttg gagtaccgga acttgtccag gtcctggcgg
     8761 atctccgagg agcagacgat gccgatgacc agggcgaagc gccgggcgtt ctcgggggtg
     8821 gagaaggccg aggccaggcg gaaacactgg gcgaagagcc agtccccggt cagcaccgcc
     8881 tccttgaccc cgttccgcac gtggaaggcg ggctcccccc tgcggagggg cgcctcgtcc
     8941 aggatgtcgt cgtggatcag ggtggctatg tgcagcagtt cgatggaggc ggccaggggc
     9001 gccaggtcgg ccgcccggcc gggcttgccg aactcggcgg ccaggaggag gaagccgggg
     9061 cggagcatct tgccgtccga ctccactacc cgggccagtt ccgcggccag ggggaaaccc
     9121 gcggagcgga cagtcgaggc catctcggcg gagacggcct cgaggcgctt cttgacggtg
     9181 gggaaatcgt tccagaagtc agtcatcgct tcgggtcgct tcgcgacccg aaggcgacga
     9241 agcccttcgg gcttcgtcga gttctccgga tcgtgtttct gaaggcaggg cggcacgtct
     9301 tcggagggga aggacggcac accgccccgg gtgcgccgtc cttccggaga tcattcgaat
     9361 cacttggtcg ggtcgtccgc gtagaagtag ttcttgcggg cgaacttggt gccgttccac
     9421 cagcgcttga agaaggggac cacccagtag tccatgccga aggcgcgtcc gaccccgccc
     9481 atgcagagga tggcggcgaa gacgaaccag agctggtccc aggcgaacat gcccgagagg
     9541 gtgaagacca cgcacatccc gatgctggcc accgcggcca gccaggtgaa gcagccgccg
     9601 aagagggcga gtccgatcag gatctccatg acgacgatca tgccctggaa ggcggcgaag
     9661 tgcaggtgcg cgaacatccc gtccatgaag gtggtccgga accaggtgga gaccgggccg
     9721 ttgaagtcga agatcggctt ggccaggtcc cagaccttgc ccagggcctt cccggctccc
     9781 tcggctacgg cttccgcggc gcccgaggcg gccgccgtgg cgtccgcggc ccattcgccg
     9841 gaggcggcgg tcaccgcctc ggcggcggcc tccacggccc cggaggcggc ggcggtggcg
     9901 tccgcggccc actcgccgct ggccgcgctg gccgcgtcgg ccacgccctt ctggaccacc
     9961 ccggaggaga acatccaccc gctcttgctg cccagatcga acttgagcca gccctcgccg
    10021 atcttgttca cgccctccat cacccacatg aggcccagcc acatgcgcag gaggagcggc
    10081 cagtaggccc ggacccggtg ggacacgaag ccgccgatga aggtgcggcc cgtggtcatg
    10141 tccaggaatt cgtgcttgag gtattcccag acctggttga tccccgcgat gttcagcagg
    10201 taccagcagt tgatcatgtg cttcatggcc atggccatga agccccgggt cttcatgccg
    10261 cccgcgttgg ccacgccgta cttcccgccg atggacacca tgaagccgtg gtagttcggc
    10321 ttgaacttgt ggtgctcccc gccgtcgatg tccgccatga tgttgtgggc caccgtctcc
    10381 gcggagcaat gggaggcctc cacgatctgg ggcatgggtt tgccgttggg ctccaggatg
    10441 aaggcggagt ccccggccag gtagacgtag gggtaggcgg tggactgcat gaagtcgttg
    10501 gcctgcatgc ggccccggcc ccgctcggcc ttggggacgt ccaggacgga ggtgaaggac
    10561 gagcccttga ccccgcaggt ccagatgaag gtgtccgtct ccacgacggt gccgtccttc
    10621 agcaggacct ttccgggctc cgcgccggtg atggcggagt tcaggaagat ctcgcagccc
    10681 atctttttca tgtagttctc ggcccggacc cggaggggct cctccaggat cgggagaatg
    10741 gagggcaggg cttcgatgtt gacgacccgg gtctccttgg gatccaggtg gtgcttgcgg
    10801 cacatgtcct ggcggtactc caggagctcg ccgatcatct cgatccccgt gaagcccgcg
    10861 ccggccacca cgaaagtgag catgcgccgg cgcttggcgg ggtcggtctc gcaggcggcc
    10921 ttggcgaaca tgtgctccac gtggtgccgc aggcgtatcg cgtcgtcgaa ggaccacagg
    10981 gggaaggagt tctcctggac cccgggaatg ccgaagtact cgggctccgc cccgaccccg
    11041 atgacgatgt agtcgaaggg gtactccgcg ctggagctct tggcgacctt ggcgtggaag
    11101 tcgaccccgg tgatggtgtc gacccggatg tcgatcttcc gggcgccgaa gatcttgcgg
    11161 aaggggacct ggaccgattc gggctccacc cgccagccgg ccacctcgtg cagctcggtc
    11221 atcagggtgt ggaagggctt cttgtccacc agggtgattt ccacgtcgtc ccgcttccgg
    11281 tacttctttt ctaggatctt cccggcccag acgcccgcgt agccgccgcc caggatcagg
    11341 atgcgcttct tgtccgccat atacgctcct tgcgtaggat tcatgatttg gttcgccccg
    11401 aaatctccat aatcttgccc ggattttgcc gttcggtcaa gtatggaata gataatatag
    11461 atataataat atatttaatc gggccggatt ccggaggcgc cgcgagcccg ggcccttgcg
    11521 attccacccg gtatccgcca atatggaaac caaggagttt gatgtgtcaa cggaagatac
    11581 gcaacacggc tttcccgccg cggaagccga ggctctcatc gggaagatcc tggaagacgt
    11641 gcgctccatc cgcgacccgg ccatcctgaa cgcctgccgg tccctcttcc gctcccgggt
    11701 ccccttccat ctccggtcct acgtcgcggc ggccctggtc cttcgtgcct tgggcgcttc
    11761 cgccggtccc cgccgctcgg tccccgtccc cgccccggga ccgaaccggg tcgtggggcc
    11821 gaagggcgaa aagcgatctc ccggatcggc gcccggtaag cccggcgctc cgtcgggcaa
    11881 ggccgggacc gagaatgccc tcgaatcggc ggacctccgg atccggaaag gctcctactc
    11941 cggggagggc acgaccctct tcttcagcat gggccgccgc caacgcttca ccgcccagaa
    12001 cgtcctgcgg gtgctctccg ggatctccgg cctcgaggaa tcggacatcg gatccatccg
    12061 gaccttcgac aactacactt tcgtcaacgt gcatcccgca gccgcggacc tcatcatcca
    12121 ggacgcggac ggcacgtcct tcaaggggcg gaagctcgcg gtcaaccggg cccgcccgcg
    12181 gaaggaagag tcccggcccg ggaggcccga ggaccgcccc gaaggggagg aagaaaccga
    12241 gggagacgac cccgtcaacg aatgatcccg cggaccgcga gcacggccag caggaaattc
    12301 gacgacaggg cggccgcgaa ggccagcccg gccaggactt catccttgcg ggccctgcgc
    12361 acgcgggcct cggccgcgtc ctccgggtcc tccgcgtccc cggggccgta cttcccgccc
    12421 gggtcctcca gtccggatct caacaggtcc ctatgcgccc ggagataccg cgcccgggcc
    12481 cagagtctgc ggtatatcga aaggaaggcc agcccgggcg gcaggaaggg gaggaccggg
    12541 gagacggtca gggcggccgc cagggccgcg ggtagggaca gcaggggcgg ccgcagcagg
    12601 tcgtagaggg ccgctcccgt ggagaggaag cccgcggtca gggacaccag ggtgacggga
    12661 ttccagtact cgttccggtg ccggcccgcc cagaccgcgc gccgggcaag atccccgtcc
    12721 cggccgtcgt ggatcaggat caacaggggc tcggcggggg aaccgaagaa gacgggaaga
    12781 ccgccctcga tcctgaggtt tccggccgcg tagacccggg ccccctccga gaggccggac
    12841 agccggttcc aggccgcggg ctgcagggat tcgtccgcgg cttcgagatc ataggggccc
    12901 tccggggtcc gtgccgacgg gagcaggtag atgcgggcgt ccttgagtag gaccgccgcg
    12961 gtcgttccat cgccgcggac ccagagccgg tcctccccct ggaggacctc cgcggagccg
    13021 gaatgccgcc gtaccaggac ctctccctcc ggggaccgca gtccgtcccc cagggcgagg
    13081 ggcgcgtaac cgagggaggc caggaaccgt ctccggaagg tcctccaccc ggcccgggcc
    13141 gagaaggccc cggcgacggg caggaccccg tagaagagca gggccgccag gaccgccagg
    13201 gtcagggacc ggagcatgcc ggtattgccc ctcccccggg gcttcgatag gataccattc
    13261 ctatgatcac gaccttgagt gtagggccta tcggagtgaa cgtccatatc gtggacctgg
    13321 gcgggggccg ggcgatcgtc gtggatcccg gggcggagcc cgagcggatc gccgccgcct
    13381 tggaaggccg gaagcccgtc tgcgtcgccc tcacccacgg acatctggac cataccgccg
    13441 gccttccggg cctcctcgcc ctcctggggc ccgtgcccgt ggcgatccac gccgcggacg
    13501 cccgctacct gggggccctc ggggaggaga ccaacctgcg gaccttcacg gatatcggcg
    13561 cccgcagctt cttcacccgc ttctggtctc ccctgcccga ggcggacgta ctcctttcgg
    13621 acggggattc ccttccgggc acctccatcc gtgtcctgca caccccggga cacacctcgg
    13681 gcagctgctg cttctatatc gagggggatt ccgccctcct ggccggggac accctcttcc
    13741 ggggcggcct gggaaggacg gacaacttcg attcctccga ggaggccatc ctggacagca
    13801 tccggcggcg catcctggtc ctgcccccgg acacgacgat cttcccgggc cacggggaga
    13861 cgaccaccgt cgccgcggaa cggccgtact tcctgtgaga gggcgcgacc ccgccgtatc
    13921 ccggcccgcg gattgaacaa accggggatt atcggaatac ttccctccat gcgccgtatc
    13981 gttccggcac tcctgtgcct ttccctttcc ctatccgcct gcgcccggac gggcacccgg
    14041 gagcccctgc ggggatccga gtacgtcctg ggcaccatct gtacggtctc cctccaggac
    14101 gggggatccg aggcggtcct ccaggcggtt ttcgcccggc tccgggagat cgaggcccgg
    14161 atgagcgcca accgggaggg caccgagatc gccgcggtca acgaggccgc gggccgctcc
    14221 cccgtccggg tgtccccgga caccctcttc gtggtcaaga aggccctgga atacgcccgg
    14281 ctctcgaacg gggccttcga tccgaccatc gggcccgtgg tcaagctctg gaacatcgga
    14341 ctggacggac agagggttcc ctcggaccgg gagatccggg aggccctgcc cctggtgagc
    14401 tggaagtccg tggtcctgga cgaacgggcc tcgacggtct tcctgccccg gtccggcatg
    14461 cgcctggacc tgggggccat cgccaagggc tacgccgcgg acgagaccgc ccgcatcctg
    14521 ggggaacgcc gggtccgggc cgcggtcgtg gacctggggg gaaacgtcct ggtgtacggg
    14581 aagaaggccg acgggtccct ctggcgggtg ggggtgcaga atcccttcaa tgaccggggc
    14641 agctacctgg ggatcgtcag catgggcgcc ggcaccgtcg tgaccagcgg ggtgtacgag
    14701 aggttcttcg agcaggacgg gaagcgttac caccacatcc tggacacccg gacgggaagt
    14761 cccgtggagt ccgagatcgt ctccgtgacg gtcatcgccc cctcctccat cgacgcggac
    14821 ggactgtcca cgaccctctt cgccctgggg cgggaacggg gcctggccct ggtccggacc
    14881 ctgcccgggg tcgaggcgat ctacgtggac gaccagcgga ggctctacct gtctccgggg
    14941 gcttccaagg ccttcaacct gacggacaag accttcacgt tcgcggagta gccgcgggcc
    15001 gttcctccgc gagacccagg cgaaggaaga cccagggagc ccccagggag acccaggcgc
    15061 ccaggactcc gtacctcagg aagcggaaga gggcgtagtt cgcggaacct tcacccggaa
    15121 ggacggcctt gaggccgaaa tagaggaccg cggctcccgc cgtccccgcg gccagccgca
    15181 gggctttccg ggcgaggccg cccgaggcgg cgtcgaaccg ggcccgggat gcggccagga
    15241 cggccccgac caccatgccg aaaagggcgc ccggcagtcc cgtctcctcc gggttcaggg
    15301 cgttcatgcc cagggccgcc gcggcggccg ccgcgatcca cagccgtccc ggtaggcgcc
    15361 cgaccagggc ctccagggta tccccccaga cggcgtggga cgccgcgaag agggctccca
    15421 gggcccagcc caggaagacg tccgtgggga agtgcacccc caggtagacc cgggaaagtc
    15481 cgatcaggaa gggcagggcc agggccagca cgaggcccca gggtgcgcgg atccaggggg
    15541 cgacgactcc ccagaaggtg gccgagctct gggagtggcc cgagggcagg ccgtagctcg
    15601 tctcccgggc cattccgacc gaggggtcca ggtcgtaggg ccggggctgc cggaagacca
    15661 ccttgaacca ggaattcagg accgcggaaa ccaggaccag gagccccagg cggattccct
    15721 tccggtgatc cacgcaccag tagaccacgg gcagggcgaa gaggaagaag tactccgtcc
    15781 cgagcaggct caggaaacgg aacgacgccg tgagacccgg ggaggcggcg gtctgcacgg
    15841 accgtacgac ctccaggccc caggccagga tctcgtccat ggacggcctc cgtgggatgg
    15901 gctgacccaa gtctagcccc cggggccccg gatccgcaag gcggcccggc cccggcccct
    15961 acttgagcgc ccggaccgag ttcagcaccg ccgtcagggc caccccgacg tcggccacca
    16021 cggccagcca catgtcggcg aaccccgcgg ctcccagggc caggaagacc agcttgaagc
    16081 ccagggcgaa gacgatgttc tgccggacga tgccccgggt cttccgggcc cgaaaaaccg
    16141 cctccggaag ggcggacaag cggtcggaca tgaggaccgc gtccgcggtc tccagggccg
    16201 cgtcagcccc ggtgcctacg gccaccccca cgtccgcccg ggccaggacc ggggcgtcgt
    16261 tgatcccgtc cccgacgaag aggatccctc ccggctctcc gttccgcttg gccaggaggg
    16321 tctccatccg ctcgagcttc tcgtggggta aaaggccgaa gtggacctcc ccgatgccga
    16381 gctccgaggc cgcctcgtgg gccggacccc gggcgtcccc ggtcaggagg acgaagcgcc
    16441 ggatcccctg ggaccggaga cggtccagag cctcccgagc gtcgggcttc acggagtccc
    16501 ccaggaggat gcggcccagg taggagccgt ccgccgcgac caggacctcg gttccggcgc
    16561 ccagctcggg ctccgggggg gcctccggca gggcgatccc ctcctcggca aggaaggccg
    16621 cggaccccgc gagcacgtgg gattccccgt cccgcgccga gacgcccctg cccgccgtct
    16681 cccggcggta gaccggggcg cccgcttccg gagcgggcgg aaggccccgg cgcccggctt
    16741 cccggcgcag ggccgcggcg atcgggtggt tggaccccga ctccgcccgg accgcgcctt
    16801 ccaggacctc ccgttccgag cggccgtccc gggggatcac ggcgcgcacc tcgaactcgc
    16861 cccgggtcag ggtgccggtc ttgtcgaaga ccacggtccg ggcctccgcc agggcctcga
    16921 aggcctggga acccttgacc aggatgcctc gcctcgcggc gccccccagg cccgcgaagt
    16981 accccagggg cacgctcagg accagggcgc aggggcagga gatgacgagc atcaccaggg
    17041 cccggtagac ccagtccgag agcgactgtc ccggcacgag aagcggcggg aggaaggcca
    17101 gtcccgcagc tgccgcgacc acggcggggg tgtaccaccg ggcgaagcgg gtgatgaagc
    17161 gctccggccg ggccttggcg tgggaggcgt tctccaccag gtccgcgatc cgggccgcgt
    17221 aggattccga ggcgggccgg atcacgcgga ccgtgagctc cccctcccgg gctatggagc
    17281 cggccaggat ctcgacgccc gggagggcgc gccggggtac ggactccccg gtcagggcgc
    17341 tggagtccac ccagccctcg ccgtgctcga ccacgccgtc caggggcacc cgctctcccg
    17401 ggcgcacccg gaccggctcg ccgacccgga cttcctcggg ccggacgacg accggacggc
    17461 cgtcccgtag gacccgggcg gaatccggcc tcagggccag gaccgcccgg atggacctcc
    17521 ggctccgcag ggccgcggac tcctggagga gctccccgat cttgtagaag accatcaccc
    17581 ccacggcctc ctcgggatgg ccgatcagga aggccccgat ggtcgacacg gtcatgagga
    17641 agagctcgtc gaaggggcgg ccggccagga ggttccggac cgctccggcg atcacgttcc
    17701 aaccggccag gagataggcc gcgacgaaaa gggcgaggac gaggggcttc gcgtccggtc
    17761 cgccggtcag gccggactcc agcaggaacc ccgcgccggc caccagggcg gcggcgccca
    17821 ggaagaggcc ctcccgggtc agggcgggat cgacggcgcg gccggggtcc ccggcgcggc
    17881 ttcgggcccc gctttcggcc tcagccgccc gggagcaggt cgcgcaggag cattctcggc
    17941 cgctatcgga ttccatggat tccatcctct tccgccaggt gcccggaagc caccgccagg
    18001 atctggcgta cgtggtcgtc gtccagggag tagaagaccg tcttcccgtc cctccggtac
    18061 cgcaccaacc gggaccgctt gaggaccgcc agctggtggc tcacggcgga ctgggacata
    18121 tccagggccg aggccaggtc gcagacgcag agctcctccg aggccagggc gcccaggatg
    18181 cgcagccgtg aggggtccgc gaagaccttg aagagctctc ccatgcggag caggcgcgcc
    18241 accgggatct gggcctcccg ggcccgctcg acgcggtcct cgtggaccgt acggcatccg
    18301 cagaccgcgg ccctgtccga atcgctcacc ttcgcccctc ccggtccgac tccgaggccg
    18361 tataaacaaa tgaacatatg aacatatatt catatatatc gcggatggtt taggttgtca
    18421 aggacggggg tgttcacata ttgccgtttc gtttaatcag actgggccgc gaattatgcg
    18481 aagttcagat aatggcgtgc acaacaagaa tatgcgcggt cccacctgtc cggccggggc
    18541 gtacgggttg ttgaaaacct tcgagtattc cagcaacccg ctagagcccc ccggccagga
    18601 cggggatgct gatgaagttg cccaaggcgc cccccaggct ggagaggaag aaaatgagca
    18661 ggatgtgggt cacccggttg cggtagaaac ccttcagggt ggagatgtcc tcgttcaggg
    18721 tctcgaaatc ccggaccgag ggcttgcgga ggaaggcctc cacgaggccc gcgaacatcc
    18781 cgaccccgat cagggggtta agggtggccg cgggggcgct caggaaggag gccaggatgg
    18841 tcagtgggtg ggccatgcag agggccgaac ccagggccgc cagggatccg ttccagagca
    18901 cccattggag cagcatgcgc aggctcaccg ccgcgccgga gcggaagaag cccgcggcga
    18961 tgaggccgac cagggccagg ggtatgatcc atcccagggc ccgggcgagg ggggagggag
    19021 ggggcacgac ctcgaggtcc gagacgtcga ctccggaacg gccttcggtg aacttccgca
    19081 gccaggcctc cagtccgtcc aggtggccgg cccctaccac cgccaccagg gaggattcgg
    19141 gctgggcgaa gatccgggcc gccaggtaac ggtcccgctc gtcgatgagg acctccttga
    19201 ccgagggcat gtagtccgct agctcccgca tcatcccgtc cagctcgtca cgctccttga
    19261 gcttctccac ctcctcggag gagagctttt cgttcgtgaa gaccccggac aggagactgg
    19321 ccaggagctt ggacttgccc cagaggccgg atcggcccca ggcccgacgc agggtggtct
    19381 gcacttcccg gtcgcagagg ctccagggta tgcccaggcg gtcggcggtc tccacggcgg
    19441 ccagcatctc cgccccgggc ttcacgccga tgtctccccc caggcggcgc tggaaggagg
    19501 ccagggccag gttggccagg agcaggaacc ccttgccctc ccggagcacc ttgccgatgt
    19561 ccaggctctc ccaggaggcg gccttggtca tggaccggta ccgcccctcg tccagctcga
    19621 cgcagacgcg cccgggcttc tcggactcga tgatggcggc ggcctcgtcg atgctctccc
    19681 gggagacgtg ggccgtcccc accaggacga aggtcctgcc tccgagctcc aaacggcgaa
    19741 cgttctgcat ctcacgctcc agaatcgaag tataaggatc gtcgcgatct acggggccgg
    19801 cgttcgtccc gcggccccgg cggccggaat cagggattgg ggcttatgcg ctccagctcc
    19861 ttttccgcca gccggtgttc caggatgccc tcccggccca tgtccttggc caggaggagg
    19921 tagcgggctc ccagggaagc cttgccttgg atcatccagt actgccccag atagaacagc
    19981 atttcggccc gcgtgtcgag gctcctctcc gcgggtatct tggtttccag ctccgagacc
    20041 gcgtcgttac ggtcctgaag gagccggagg atgagccagt ggaggttctg ggtctcccgg
    20101 ctcacccggg ccaggtagcg ggcggccagg gccttgccct ccgcgtcctg gccggcccgg
    20161 taggcggaga tgaccgccag gatgccgtac tccatcctgc cgggggaata ggagagggcc
    20221 ttttcgaacc cctcccgggc cgccgggaaa tccccgagac ggaaggaggt gacgccgatg
    20281 gactcgaagg cgtaccagta gtccggatag agggccgtga tcctccggta gtccgcccgg
    20341 gactcctcgt cccgccccgc ctgctccagc atccccgccc ggtacacgta ggggaggaag
    20401 tagtccgggt ccaggcggat ggcctcgtcg aagtccgccc gggcctcgtc caggcggccc
    20461 atgtccatcc ggaagcgccc ccgctcgatg cggtaccagg ccaccgccgg gtccagccgg
    20521 acggcctcgt ccaggtccga aaggctctcc gcgtacttgc ccctgcggta gagcacccgg
    20581 ctccggtccg cccgggcctg ggaggaggcg gggtgggact ggaccgcctg gttgaggacc
    20641 agttcggcgt ccgcgggctt gtcccggcgc aggagggtcc ttcccaggcc caggaggccg
    20701 gagaggttct gcggatccga ggcgacgctc ttccgataga gctcctccgc cttggtgtag
    20761 ttcttgccgg cgtaccagga gtccccgtag gccgccaagg cctccgcgtt ggccggatcg
    20821 accgccagga ccttgtccag cgcggcccgg agggccttgt cgtccccgga gtagcgttcc
    20881 acctggacca ccgccatcag ggcaccggga tccgcgggcg cagcggagac cgcctcggcg
    20941 gccgcggccc gggcttcggg taccttgccc gcggagacca ggacggagga aaggaggatc
    21001 cggtactcgt tcttcccggg gtcctccgcg atcagggcgc gcaggcggga ttccgccgcc
    21061 gtgaagtccc ccttggacag caggtcggac agggactcca tgctccggat ttcccgctcc
    21121 cgggggctca gggccgaatc cgggaccttc tcgggaacgg gggcgcgttc cggcccgccg
    21181 acgcaggagg cggccaggag gatcgcggcc aggaggatcg cggtccggaa ggccgcgggg
    21241 aagattcggt ttcctcgcat ctgattctcc gttcgctcga gttccggcag gttgtcgaaa
    21301 tgcatcgagt atttccgcaa actgccgggg agcgcccatg gtacgggttt cccgggttcc
    21361 gcgcaagctc gccctccagg ttgcaatcgg ggggaaaacc tgctagagtc cgcccatacc
    21421 atgaaactag gcgcgttcgg acgaggcttg gccctcgccg ttctctatat cggcattctc
    21481 ctcggcctgg tcctgctcca attccgggaa ggcggggact tcacccgggt gtcttcgggg
    21541 atcaccctga agggccgggc gggcgagcag gaaggatccc tggtctccgg aaccctcagc
    21601 ttcgggggcc tggtccttgt cttttccccc gccaagcccc tggcctggac cgcccaggac
    21661 gggggctcgc gttcggccct gccgaagtcc ttccaggaaa ccaaggacgg gttcctgatc
    21721 cagttcacgg acggcctgga gctccgggcc cgctcctcgg gcgagccggg ttcccgggca
    21781 agcttcgagt tctcccgccg gacgggtccc ctgaaggacg tgaccgtctc cgtggccgcc
    21841 gcccgccgct tccgcctcgt ccaggactcc aggacctacc tggtttccgg aagcgccggc
    21901 cggtacgtcc tggaagtccc ggagggcacg gtctccccgg accggaagag cctttccctg
    21961 cccttcaccc ggggtcgggt ggcccccgta gccctctcca aggccccgga cgaggcccag
    22021 acggcctcgc cgcgcttcct ggagcaggcc gccctggatc ccgaagtctt ccagcgggag
    22081 atctccgcgt accgggacaa ggtctggaag gggctgtcct ccgaccgctt cctgccgggg
    22141 accgtggaat ggagggacgc ggcgggaaag gcggccttct cggagcccgc cctgacggcg
    22201 tacctggcgg aagcctggtc ccggggccag ggcccctccg ccttcgagcg ggtccaggcg
    22261 gccaacaccc tgcacgcctc gtccctgacc cacctctccg ttcccttcct gggccgcacg
    22321 acggaccgga tgcgcgcctt cgaggaggcg gacctcctgg aggtcaagcg gatcgagcgc
    22381 ctggtccagg cgaagaaccc gtccctgctg gaaaagccgg ggctcgtgcg attcctcctg
    22441 gaccgggcgc cctacgccct ggcccgggac gccttccaga tggccggggc cctggacctg
    22501 ggatccctca ccgtggccca ggccgccggg ggcctggccg cctgggcgga gtcggaggac
    22561 ctgttcccgg ccggggagaa ccccttcgcc cggttcgcgg gcatcccgga gcgtcagatc
    22621 cttcccgccg tggtccgcgc gggggacggg tacttcctga gcaccggccc cgacggatcc
    22681 tcgcacctgg agacctcgat ccgtgcggga ctgtccctga tccgcctggg cgagcccacg
    22741 gccaagccca tgttcacggg catcggccag agcctggtgc tgggcgccct tcgcctggcg
    22801 gatcccctgg gcttccttcc ccggaccgtc gttgtccgca acggggccgt ggcggacaga
    22861 ctggaaatcc tctcccccga ggaagcctac cccctgctgt cggggaattt gtactatccc
    22921 cgggaggtgt ccttcttcaa ggaggtcgga cccggggcct ggatgtggac ctgctcgccc
    22981 gccgtctcga tctccgccac accggagagg atggtctgga ccctggaatt ccccgagcag
    23041 ggagtccact acatcgcggc ctacggggtc aagcccttcg ccaagatgca gctatacggt
    23101 ctggactacc ccatggaccc ggccttcgag aactacaacg cctcgggcta cctgtatcgg
    23161 cgcgcctcca acgtcgtgta cttcaagatg cgccacaagt cccgcaccga ggacatccgg
    23221 atgttctact agacggtccg agcctccgcc cggaaccggc ggttcacttg cccgcagcct
    23281 tccgggcctc ttccgcggcc ttggccagga ggtccgcaag cctggccaac gtgtccgccg
    23341 gcaagccgtc catccggccc tgcagttcct tgaccagttt ttccaattcg acggccttgg
    23401 ccagggccgc gtccccggat gccgcccgga ccgccagttc gtcgatacgc cgggcgatct
    23461 gggcgttgtt ctcctccacg gactggaccg tcgcgacccc gagggtcttc cagaccgtcg
    23521 cgcacccggt caacaggacc cccagggcga gggtcagcag ggcatacttt ttcatatccg
    23581 gctccttgcg tgctcggtgt cgacggcatc acggccgcct ccccattcgc gatcctagga
    23641 cggattccgg ccggccgcaa gtcccgggat ccggaacccg cggccggagg atgtcccgtc
    23701 cgacggcacg tccggaggag agcccgcctg aggattgtcc cggcggagcc tttttattat
    23761 catttctctc agcaaggaga tacttatgag aaggtccata ttgctgttca tcgccctggc
    23821 gtcggccgcc gcggccgtcc aggcccagcc caagccgatc tccccccggg ccgcccggga
    23881 tcttctgtcc gccgacaaga acgccctcct gctggacgtc cggacccggg aggagttcct
    23941 cgaggtccgg atacccggat ccgtgctcct gcccttcgac gccatcgacg cggccagcgc
    24001 ggcccgggtc gtgggcccca aggaccggac ggtggtggtg tactgccgga ccggccgccg
    24061 gagcgccgtg gccgcctcga ccctggcctc cctggggtac cggaacgtgc acgacatggg
    24121 agggatcggg tcctggccct tcgggaccgt ccggggagag tagcgcttta ttccatggaa
    24181 ttatgctgtg aaccgccgag gccgtaggct tcggcgcccg gtgttcccaa tacgtttcca
    24241 acgtattggg aacacctcgc tatttcatga acaaccccgg caggaactcg gtgaaccagg
    24301 gaacataggt gatcaggagc aagaccacga tcatggccgg ccagagggga agtaaggccc
    24361 tgctcgtcct ttcgatggtc gtcttgccca cgatgcatcc cacgaaaagg ctcgccccta
    24421 cgggaggcgt ggtcaggccg atgccgaggt tcagcatgag caggatcccg aaggtgacct
    24481 cgctcatcct ggcggccttc actacgggaa gcaggaccgg cgtcacgatc acgatcaggg
    24541 gggccacgtc catcaccatc ccgagcagga ggatcaggat gttcaccagg agcaggatca
    24601 ggaagggatt cttggttaag ctgaggaagg cgttcatgac ggcggcgggg atctgcaggt
    24661 aggtcatcat ccatccgaag gccgaggcgc agccgatcag gaaaagaacg atggccgtgg
    24721 tcttcacgga ttccatcagg atgggccaca ggtcgcggat cctcaactcc cggtacacca
    24781 cgaaccccag gaagcccgcg tagaccacgg acacggcgga agcctcggtg gcggtgaaga
    24841 taccggccgc gatgccgacc acgatgatca gggcggccag gagggccagg atcccgtccg
    24901 ccgcgatgcg gaggcttctc ttcaggggag gccgcccttc gcaggggtac ttcctcttca
    24961 gggcgatgat gaagcaggcc gcggaaaggg ccagtcccag caggattccc ggtatgtatc
    25021 ccgctaggaa caatcttccc accgatactc cgcccgccac catggcgtag atgaccatgt
    25081 tgtgggacgg ggggatgatg atcccctggc tcgcggaggc gacggtgacg gccacggaga
    25141 aatcccggtc gtaccccttc tccaccatca tcgggatcat gatgcttccg gtggaggaga
    25201 cgtccgcgac ggcggagccc gaaaccccgc cgaagaacat ggactcgatg cagttcacga
    25261 tggccaggcc tccgggcagg ggtcccacca ggatataggc gaaatcgacc atgcgcttgg
    25321 ccactccgcc ccgggccatg atggagcccg ccaggatgaa cagggggatg gccaggaggg
    25381 ggaaggactg catgctcgcg gccacccgtt gggcgatgac ggcggggccc atccccaccg
    25441 ccgtggcggc caggagggag gagatgccca ggctgaagga aatgggaatc cccaggagta
    25501 tcagcagggc gaaggacccc aggaggatga cttcaacggg catggtcgcc ttccttccgg
    25561 ggggaacggc tctccagggc ctccgccgcc ttgcggagta tccggagcgc cgcgaaggcg
    25621 atcatgaagg ccgccgcgat cgggaggacc aggtacgtca agcccagggg caggggaatc
    25681 gtggacatcc ggctggtcca ggtggcttcc gtgagcagcc ggccgttcga gagcaggaac
    25741 gcggcgaaat atacgatcgc cgcgtcgatc aggatctcca gggctaggcc gtagcgtttg
    25801 ggcagtctct tcgtgaccca ttccaggcgc atgtggtcgc cggtccatac ggcggccgcc
    25861 gcgcccagca ggccgatcca catgatcgcg gtcacggaga attcctccac ccagacggga
    25921 ctcttctgca ggacgtaccg cgcgaaaacc tgccagcaga cgatcagggc cagaacgccc
    25981 atgatcgccg ccgcgatacc ggtcaggacg cgctcaagcc ggtcgaggat cctggaaaaa
    26041 aggtcgggca ttgggttccc ccgtggaagc tgtgtcgcca tgattatacg actcccgggt
    26101 catcccgtgc gtaccccgca caggattccc gggagccgcg gattcggatc cagtccgaac
    26161 ggtccggtct atttcgcggt cacgatcgcg tcgatcaagt ccttgccgac gatcggttcg
    26221 aactctttcc agacgccccc gacggccttc tggaactcgg agatgtcctt cacctcgttg
    26281 aagatcatcc ccttggcctt gaggtccgcg acggtcttgt cgatcatctc gccccagagc
    26341 ttgttgtgct tctccatggc ctccagggcg gtctgctcga tcatggtctg caggttcttc
    26401 ggaaggccgt cgaagagctt cttgttcatc atgaggaagt cggggatgga ggagtggcgg
    26461 gtgtagctgt agtacttggc cacctcctgc atgttgaagg acaggacggt gggttcgttg
    26521 ttctcccacc cgtcgatcac cttggtctgg agggcgctgt acacctcgga ctggcccatg
    26581 gggaccgcgt tgccgcccag ggcgttcacc gtgtcgatga ggggcttgct ctgcatgacc
    26641 cggatcttga gccccttgag gtccgcgggg gtattgatcg ggcccttgga ggtcgtgatg
    26701 gaccggaatc cggcgtccat gaaagccaag cccttgtatc cggccttgtc caggtcggcc
    26761 atcagtcgct tgccgatggg cccctgcagg accttgtgct ggtgatccag gttccgccag
    26821 atcaggggca gggagatgac ctggaaggtc ggggcgtact gtcccaccgg cccggaggac
    26881 accttcgcga attccaggga cccgatcttg atggactcga tgtagtcctt ttcctggccg
    26941 agctgtccgg cggggaagag ggtcagcttg atgtcgcctc cactcttccg ggcgagctca
    27001 tcggcgaaga agacgagggc ctggtggacg ggatgggtgt ccggcaagac ggacgcgagt
    27061 ttccagttgt acttgggagc cgccgtagcc agggtcaggg cgagcgccgt gagaagaacg
    27121 catgccagga tacgtttcat gatggcctcc ttggtcgatc cgttgttccg tattgtggaa
    27181 taacgtttca cgatcaagaa aagtgtacga ccgtttcttt aaatgcgcaa gcgatattcg
    27241 gaagcttccc ggacgccgcc gcgcttgccg ccgtccccgg gggggattag actgggatcg
    27301 gcaaggaggt acgggatgga tcgactccgg gggaaaaagg ccttggtgac cggcggcgga
    27361 tcggggatcg ggaacgctat cgtgcgggaa ctggcggacc ggggtgcgga ggtcctgatc
    27421 cactacttca agagcgggga ctccgcccgg gccctggtgg acacgatccg ggcgaacggc
    27481 ggcgcagccg aggccgtcca ggccgacctc tccgccgagg acggtgcccg gtccctggcc
    27541 gggttcgtgg agcggcgctg gggcagcctg gacgtcctgg tgaacaatac cggggatctc
    27601 gtgggccgcc ggaggctcga ggaagcggac ttggcattct accgctccgt gatggctgtg
    27661 aacctggact ccctggtgtt cgtgacccgg gccctgatcc ccctgctccg gaagcccggg
    27721 gccgcgtcca tcgtcaacct gtcctccctg gccggccgga aggggggcca cggcggctcc
    27781 ctggtctatt ccaccgccaa gggagcgatc ctgtccttca cgcgctcgct ttccacagaa
    27841 ctggccccgg acggcatccg ggccaacgcg gtggcccccg ggctcatcct gggatccgcc
    27901 ttccatcgga tccacaccac ggaggaatcc aagcaggcga ccatccggga catcccgctc
    27961 cgccgcgccg gcacctgcga ggacgtcgcc cgggccgtgg ccttcctggc cggcgaatac
    28021 gacgggttca tcacgggcgc caccctggac atcaacggcg gggtctacat ggcctaggag
    28081 cctgtggact tgtggatttt tagcgccgat aggcgctaaa aatccacgat tatccggctc
    28141 ctttggagtt tgcaaggtcg gggtgtccaa taagtcggtt acttattgga cacccccttt
    28201 attttggtgg gcaagcccga caaactcata gcgcacgggt tcggcacggg acggccgggc
    28261 cccggcggac ctgtcccgaa ttccgccgtc tcagccggcc gccctgccgt gcaccctctc
    28321 ccgttcgacg taggcggtgt ggagcaggtg gtgggccttc tccgagcagg gtgagccgag
    28381 gaaggtctcg tacagccgct ggaccgcggg attctcgtgg gacttgcggc tcggaagggt
    28441 ccggtcctcc tcgtacaggc cgcttccccg ctccgcccgg gtgcccaggg tcgagccgaa
    28501 gggctgtccc cctcctccga cgcagccccc ggggcaggcc atgatctcca cgaagtgata
    28561 ctcgctcctt cccgtcgtct caagctggtt ccggatcacg tccaggatcc tgcgggcgtt
    28621 cccgaggccg tgggccaccg cgaccttcaa gtccagggtc ccgccgtccg gcagagggag
    28681 gggcacgacg gcttccttga cgccctccat gccccgcacg ggcaggatgt ccacgttttc
    28741 cagctcccgc cccgtaacca ggtggtaggc cgtgcgcagg gccgcctcca tgacccctcc
    28801 ggtggccccg aagatggtgg ccgcgccggt gtacgagccc atcagctcgt ccgcgggggc
    28861 gtcggcgaga tcctcgaatc gcaggccgga ctccttgatc atccgggcga gttcccgggt
    28921 ggtcagcacg tagtccacgt ccttgaagcc gctggaccgc atctccgggc ggtccgcctc
    28981 gaacttcttg gccgtgcagg gcatgaccga gaccgagacg atgtcctcgg gtttgagccc
    29041 cgcctcccgg gggtagtagg tcttggccag ggctccgaac atctgctggg gactcttcgc
    29101 ggaggagact aggctccgga gcccgggata gaaggtctcc atgaacttga tccagcccgg
    29161 ggagcaggat gtgatgaccg gcaggtccgg gctccccgac aggatgcgcc ggacgacctc
    29221 ggtcccctcc tccaggatgc agacgtccgc ggcgaagttg gtgtcgaaaa cccggtcgaa
    29281 cccaagtttc cgcagggcgg cgtgcatctt tccggaaacc accgctcccg gggataggcc
    29341 gaactcctcg cccagggccg cccggatggc gggcgcctcc tgtacgacca cgtgcttccc
    29401 cggatcggcg aggctgcggc ggacctccct cgtgtcgtcc cgggaggaca gggccccgac
    29461 ggggcagtag accacgcact ggccgcagtt cacgcagacg ctgtcctcga tcggaaggtc
    29521 gaaggcggtt ccgatcttag aagtatagcc gcggccgatc cgggtgaggt tggccacccc
    29581 ctgcacttcc gtgcagacgg tgacgcagcg attgcacagg atgcacttgt tggggtcccg
    29641 aaggacgatg ccgccgtcgc tgcgccggga gaagacctcc ttgggggcca tgcgaatctc
    29701 ccggatgccg aggtcctggc cgatcctctg caggtcgcac ttgccgtgct tgtcgcagat
    29761 ggggcagtcc gccgcgtggt ccgccaggat cagttcgatc agggtccggc gggtgtccat
    29821 cacccggggc gtgttggtcc ggatgtccat gccatccccg gcgggggtgc agcaggaccg
    29881 acgcactccc acgccctcga tctccaccac gcacatcccg caggtgccgt tgggccggag
    29941 gtcgggatgg aagcacaggc tggggatctc gtatccggcc gtccgggcag cctgcaggat
    30001 catcgtcccc tcggggactc gaacccggac cccgtttatg gtcaccgcga catcacgacc
    30061 gtccatcgcc gggcctcctg tcgttccttc aggaccttga agcggcacac ccccgccctg
    30121 catttcccgt tcacgtgctc ctcgaattcc tcccggaagt agcggatcgc ggtgctcagg
    30181 ggcaggaacg gcgactgccc cagggcgcag tacgaggcga ccttcatggc cgacgcgatc
    30241 tcctccagga ggtcgatgtc ggacgggcgg gcggatccgc tgcggaaacc gttcaccgtc
    30301 cggaccagcc gctcgttgcc gatccggcag gggaagcagg ccccgcagga ctcgtggagg
    30361 aagaaattga ggatcccgtg gaccatgtcc acgatgcagg tggtctcgtc catgaccagc
    30421 atggacccgg aacccaggat gccgcccttt tccttgaggg cgtcgaagtg catggcctcg
    30481 tccaggacgc tcgggtcgaa gatccccccg gaggagccgc ccacctggac cgccttgagc
    30541 cgcctgcgtc cccggatacc cccgccgtag ccgtagatga gctcccgcag gctcgtcgtg
    30601 gtctccacct cgaccaggcc cgggctgacg acatgcccga tgatcaggta gatcttggtg
    30661 cccggggagc gctccgtgcc gaacttccgg aaccagtcgg ccccgttgcg caggatcggc
    30721 gggacgttgg ccagggtctc cacgttgttc accaccgtgg gccgtcccca gagtccctcc
    30781 gtcaccgggt agggaggctt cagacggggg gtgccgcgct tcccctccag ggactcgatc
    30841 agggcggtct cctccccgca gatgtaggcc cccgcgccct ccttgaccct cacctcgaag
    30901 gagaagggcg agcccaggat attccggccc aggtaccccg cttcccggga cttacggatg
    30961 gcgttctcca ggcggtcgat cgaggtccgg tactctcccc ggacatagac gtagccctcc
    31021 tccgctccgg tcgcgtagcc cgccagggcc atggcctcca acacgcggaa gggatccccc
    31081 tccaggatga tccgatcctt gaaggtgccc ggctcgccct cgtcggcgtt gcagatcacg
    31141 tacttcctgt cccccggggc ctcccggacg gcctcccact tcatccccgt cgggaagccc
    31201 gcgcctcccc ggccccgcag gccggatcgc ttgacggtgt ccaggacccc ctccggggtg
    31261 ccgccccgca ccgcttccgc cagggcccgg aagccgtccc gggacttgta ctcggccagg
    31321 ttttccgggt cgacggcccc cacgttgtcc aggaccacac ggcctcggcc cttcttgatg
    31381 atcccttccc gccggcgcca tgccatgagg cgccgcatct ccggcgagtc caggaagagc
    31441 tcctccacgg gcttgccgtt gatgaagtgc tggtcgacga tgcggtccgc gtcggagagt
    31501 ttcaggttcc cgtagtacac ttcgtcgggg tagacgagca tgcagacgcc ctggccgctg
    31561 acgccgaggt tggcggattc cacgatccgg acgtcctccg acaggaactc cttccgcagc
    31621 ttggcttcca ggacctcccg gaatttccgg gcgccggcct gctccgcctc cgcgcccacg
    31681 tcgaggatga cgtgcatcag ggggatcatg gctcctccct tcgcttccga tcgaggatct
    31741 cccggagccg gaccccgtcg aggttcccga agatctcccc gtcgatcatc atggccgggg
    31801 cgatgccgca ggttcccaga cagctggaga gctccagggt gaacaggccg tcctcggtcg
    31861 tgtcgccgaa ctccaccccc agatactcgc ggagttcgtc ccggatggtc cgcccgccca
    31921 tgaggtggca gggcggcgag gcgcagaccc ggacgacatg gcggccccgg ggcttcacgg
    31981 aaaacatcga gtagaatgtc gccactccgt agacgaagct gtacggaagc tccagataat
    32041 cggcgacctc gcggagttcc ggctggtcca ggaagttcgc gggattcgcg tcctgtaccc
    32101 ggtgcaggat cgccaggagg ttgtcctggg tcggttccag ggaccggatg atctggctcg
    32161 tcgtcatgga ggatcctcct ttcgacggaa agaacgggat gagagggtgg gaatggagga
    32221 gggttcggag ggaagaaccg aggatccttc ggacaccgag gaaacctccc cggtcgggat
    32281 tccgtgcatc ctggttgtcg tggcaccggc cgtgcgcggg agcgtcttca ggggctcccg
    32341 cgcgtaatga ttacgttgta tccccgtgcc gtttcatctg tcaatacgaa cttcgggctt
    32401 tcctgcgggc ttcgcgggtt ccggcaccca aagcgccgcc agggcgccca gaaggcccgt
    32461 ggatacggac acttcccaga aatgggccag gctaaccgcg gggggcatca agggattcgg
    32521 gaaggccaac tgggccgtgg gcaggattcc gagggcgagc ccggaaagga tggtcagttc
    32581 cacgcggccg cccgtgaatc cccggaacag gggcaccagg cacaggaccc agaggcagcc
    32641 ccggaaccac tggaagaccg ggatctcggg tttttccatg aaggatcgtc cccattgggc
    32701 gaagaatccg agcttgtccg cgttcccgga gtagaagagg cggaccgcct cggactgcca
    32761 ggcgacgtag tacccgaaga gcatgtacag gaccaggtac gcggccccga ggatcgccgt
    32821 ccgggccagg gcgcggaccg gatccacggt gaaccggggc tcccggagct cggcggtctt
    32881 ccggaaccct ccggtcagaa gagttgccgc cgccgcggcg gcgaaacccc agagcgctcc
    32941 ccgcaggacc atcctgtagg tttcgaagga tccctgtagc aggggaaacg cctccagaaa
    33001 ataggccgtc tcgatctggg tcatgaaagt ctgggccccg attaccagga cggctacctg
    33061 gcctacccgc ttccacccgg gcaccgcgga cctgcgggcc gtccagtacg ccagtagggc
    33121 cagggagccc aggaaggaga ggaaatccag gggggccggg aggaatccgg aggcagggac
    33181 agcggcccgg atcgcccccg ggaccgggac ggccaggctc gcggcgatga aggcggcccc
    33241 cgccaacagg agggtggcca ggaacccgcc ggtgttcctc aagcccttca ggaatgcacg
    33301 gtctcttgtc atgggactct ccttacatcg gtcaggcccc gcgccgtgca ccgggcgaac
    33361 agggcgatca ccgcgtcaaa ccggtcttcg ccgatctggt cggcgtgaag gtataggaag
    33421 aagtgggagg tcagaagcag ggccagggtc cgcgggtctc cctcgacgag gagtccccgg
    33481 gcctgccagt cccggacgaa ggtcaccatg gcccccaggg aggcttcgtc caaggctttc
    33541 caaacctcct ccggaagctt gcgtctcagg agggcccggt cttcgggatc cgtcatccgc
    33601 cggagcaggg ggtcggaccg gaacgcttcg agggccccct tcatgagggc ttccagggtt
    33661 tccgccggtc gcgaggtcat tgcggagacc cttcggaagg cctccgcgtt cgccgcgtag
    33721 cgctctctga gcaactcggc atacaggcgt tccttggaat cgtagaacgc gtagaaactg
    33781 cccgaggcga tgccggcccg gccgcagagt tcctggatac ccgttccccg gatcccccgc
    33841 cggccgaact ccaccgcggc ggtttcccga agcgcgttcc ggatcctgct tcgctcttct
    33901 tccaaaaagg gtttcatgaa gactcccatc agtataaatg aatgtataat tcattcattc
    33961 acagatgtca aggtactttc ccttcttctt cccggggagg aatacgggac ggccgcactc
    34021 gagatcgcct cgtgctgcga atacagggtg gaggatccac tcctgttgaa aaatgatcga
    34081 aagataaaga aaagaacaat taacttgcat tattccaagt atcgatctac aatatgaaca
    34141 gaaaatagct cgacacgctc aaataacgct caaaaggagt atccaatggc cctacccctt
    34201 ctccaactgg ccctggacaa caagaccctt cccgacgccc tggtctccac ccggctcctg
    34261 gcttcggaag tcgacgtcct ggaagccgga accatcctct gctgttccga ggggatgcag
    34321 gcggtgcgct gcctcaaggc cctgtacccg aatcgcatcg tcctggcgga cataaaggct
    34381 gcggacgcgg gcaccatcct ggccaacatg gtctacgacg ccggggcgga ctggatgacg
    34441 gtcatctgct gcgcccccat cgccacggtc gagaccgccc tggcggccgc caaggcccgc
    34501 aacgaagcgg gaaagaccgg cggcgtgcct cgggacgtcc aggtcgagct gacgggatcc
    34561 tggacctggg aggaggccgc cgcgtggcgg gccgcagggg tcaagcaggt cgtataccat
    34621 cgagggcggg atgcccaggc cgccggccag tcctggggcg aggaggacct ggccaagatc
    34681 cgccgcctgg cggacatggg cttcgacctg tccatcaccg gcggcctcgt ggaggaggac
    34741 ctccccctgt tcaagggcat ccccgtgaag gtcttcatct ccgggcgctc catccgggac
    34801 gccgcggatc ccgccgccgc ggcccggtcc ttcaaggccc ggatcgccga gctctggggt
    34861 tagtcgtgga cgagcggctg cccctgcctc cccggatcgg catctacgag aaggccctgc
    34921 ccgcgggact cgactggccc gggatgctcc gggcggcccg ggaggcgggc tacgactacg
    34981 tcgagatgtc cgtggacgag acggacgccc ggctggcccg cctggagtgg agcgccgggg
    35041 agcgggccgc cttccgcgag gccgtggcgg actccgggct ccgggtgccc tcgatgtgcc
    35101 tgtccggcca ccgcctgtat cccttcggaa gcgcggatcc ggcggtgcgg gagcgctccc
    35161 gggacatcat ggtccgggcc ctggacctcg cggcggacgt cggtatccgg accatccagc
    35221 tggcgggcta cgacgtctac tacgagcctt ccacccccga gtcccgggag aggtaccggg
    35281 agggcctggc cgaggcggtg gagctggcgg aacgggccca ggtctgcgtc gccatggaga
    35341 tcatggacac ccccttcatg gggaccgtct cccggtggct cgagttcgcg gagaaactgc
    35401 cttccgcctg gttccaggtc tacccggaca tcgggaacct ctcggcttgg gccgaggacg
    35461 tgcccgggga gcttgcgaag gccaagggac gcatcgccgc ggtccacctc aaggacaccc
    35521 tgaaaccccg gccgggcttc ccgggtcggt tccgggaggt gcccttcggg accgggtgcg
    35581 tggacttccc cggggccttc cgcgccctgc ggtcgatagg gtatcgtggc tcctacctga
    35641 tcgagatgtg gacccatcag tccccggacc cggccgcgga gatccgggcc gcccgggagt
    35701 ggatcctggg ccggatgcgg gaaggaggcg tcgcatgacc gtcgaggaac tgaaggaagc
    35761 cgtgttccgg gccaacctgg agctccggga ccggggcctg gccatcctga cctggggcaa
    35821 cgcctccggc atcgaccggg agcgcgggat cgttgccatc aagccctcgg gggtgcccta
    35881 cgacgtcatg aaagcggcgg acatggtcct ggtggatctc gacggcaagg tcctggaagg
    35941 caagctccgc ccctcctcgg acctggccac ccacctggtc ctgtaccggg ccttcccctc
    36001 cctggggggc gtggtgcaca cccactcggt ccacgccacc gccctggccc aggccggggc
    36061 cgacctggcc gccgagggta ccacccacgc ggaccacttc tacggtcccg tgccctgcac
    36121 ccgggccatg acccgggcgg agatcgaggg ggagtacgag gccgagaccg ggaacgtcat
    36181 cgtggagacc ttccggaccc gggggatcga ccccgcccgg gtccccgcgg tcctggtgcg
    36241 cagccacggg cccttcgcct gggggaagag ccccggggac gcggccatga acgccgtggt
    36301 cctggaggag gtggcccgca tgtccatcct gtcccggtct ttcggcgatc ccgggcccat
    36361 gcccaaggag ctgctggatc ggcactacct gcggaagcac ggggccggcg cgtactacgg
    36421 gcagaaataa gcggaagggc ggccggtatc cgcccggcgg atctcggcct atgccctggc
    36481 cgccaataaa gagaatgatt ccttcccgat cccttgatta agtcctttta tatagatagt
    36541 atcccggcca tgtcggaccc cctcaaccaa ccccacagca agaccagcat cgtcgtggcc
    36601 aacccctcgg ccatgggcct cttcggactg gccatggtga ccctggtcgc ctccacccag
    36661 aaactcggct ggacctcggg caccgccttc gagttgccct gggccctctt cctgggcgcg
    36721 tccctccagc tctacgcgtc catcctggac gccaagctga acaacgcctt cggcgccacg
    36781 gccttcggag gctacgcctt cttctggttc gccgtcggga tgacctggat gatccagggc
    36841 ggctggttcg gggccgaggt cgcggcccgg gcggatgtcc gccagttggg cgtcgccttt
    36901 ttggggtacc tgatcttcag cctctacatg accctggggg ccctggccac gaccaaggtc
    36961 ctgttcggga tcttcgtgct gatcgacctg ctcttcctgg gcctgtccct gtccgtgctc
    37021 gggatagccc gggagttctt ccatgccctg gcgggctggg cggaactggc catcgccctg
    37081 gcgtccttct acgcctccgc ggcggccatc ctgaacgccc agttcggccg gacggtcctg
    37141 cccctcggaa aggccttcgg catccgccgc ggctgaccgg tagctccgcg gaacgcacgg
    37201 ccggccgttc gggcccgaag ccgcccgcgc ttccgatgcg gcccaaaaag gcccccggtt
    37261 tcccgggggc ctttttgcat actgcggata gacgatccgc cgacctcgcc gaaaccggat
    37321 ggtttcggcg aggtcggccg cccgcgtccg ggcgctacag gaacgagggg tacggcaggt
    37381 ccgtcgggcc ctcgaagacg tcctcgaagc cccggggata gtggtactcg atccggtccc
    37441 ggtcggcggg ccaggtgaac ttgcccccca cctgccagac gaagggggtg aagccgtagc
    37501 ccagacggtc cttccgcatc ctccacaggg ccaccacttc catgggatcc gcctggaagt
    37561 tggtccagat gtcgtggtgg aaggggatga ccgtcttggc gttcaggcac tcggccatgc
    37621 gcaggatatc cacgctggtc atcttgtccg tcacgcctcg ggggttctcc ccgaaggagc
    37681 ccagggccac gtcgatccgg tgctcgttgc cgtgctttgc gtagtggttc gaatagtggg
    37741 agtccccgga gtggtagagg gagccgccgg gggtcttgaa gaggtagttc accgcccgct
    37801 cgtccatgtc ctggggcatc ctaccggaaa ggccctggtc cgcgggggcg gtgatcaggg
    37861 cggtccggtc gaaggactcc agggccagga tctccacgtc cttcaccttc accgtgtccc
    37921 cgggctttac ggtcacgcag cgcgtttccg gcactcccca ggaggtccag agcttcacgc
    37981 aggcagccgg cccgacgaag ggcacggccg gatcgcagtt cttgaggacc gcggcggcca
    38041 cgttcacgtc gatgtggtcg ttgtggtcgt gggtggccag gatcgcgtcc acgttccgga
    38101 tggcgaaggg atccaggggg aagatcgagt tccggaggtt gagctggagc ttcttggccc
    38161 ccgtcatcct ctgcatctgg tgccccgcgg ccatcacggg attcttgtgg gtcttctttc
    38221 cggtaccgac ccagaaatcc gtgcagagat tcgccccccc gggggacttg agccagatac
    38281 ccgtacagcc cagccaccac atggcgaaag tgccgggtgc gaccttttcc tcctcgatct
    38341 cctcgttgag ccaggtcccc cactcgggga aggttcccag gatccaggat tcccgggtga
    38401 ttccgtctat ctttgccaaa acgttcctcc tgcgattcgt tctgtcgttc tatcccgatc
    38461 ccgggacggc gccgcgcccc gggaccgatc cgccggcccc cggctaggcc ctcgagaggg
    38521 tccgccgctt gggcaccagg tagccctcgt tcaggagcag ggccaatatg atcaccgagc
    38581 cggtcacgat ggggtggaac ggcgccggga tgttcatgag gttgaggccg ttgatgatga
    38641 aggccaccat gatggccccg atcagggcgc ccagcacgga accccgtccg cccacggcgc
    38701 tggtgccgcc gatcaccagg gcggtcatca cgtccagctc gatccccgag gccgcggccg
    38761 cgaagacctg ctgggtcagg gaggccagga tcacgccgcc cacggcggag gtcccgccgg
    38821 agatcgtgta cgagatcagc cgaacccggt cgggatccac cccggacagc cgggcggcca
    38881 cctcgttgga acccacggcc atgacctgcc gcccgaaggc cgtcctgcgc aggacgaact
    38941 ggctggtcac cgccagggcc accatgatgt agatggggtt gggcaggccc aggaagcggc
    39001 ccgtgctgat caggatgaag ggccggggct ccaggatggc cagcatggcc ccctgggaca
    39061 ccaccagggc gatcccgtag aggtaggtct tggcggccat ggtgaccacg aaggccggga
    39121 tcttgatctt ggccacgatg aagccgttga acaggcccac cagggcgccg atgaccacgg
    39181 tcatcaggat cgagggtatc caggggattc ccatccggtt catgaagagg tccaccacga
    39241 ttcccgagag ggccgccgtg gatcccaggg acaggtcgat gcccccggtg atccgcgggt
    39301 agatggcccc ggaggacagg atgccgatga ggcacaccga ccagaccacg ttggtcaggt
    39361 tgtccagggt caggaaccgg ggggacagga cggacaatac cgccatcagg ggtaccagga
    39421 cgacggcgag gcggagctcg gtggcgatcg gtttcttatt catcgtctcc ctcccggatg
    39481 ccggacgcgg cgcgaaggat cttctcgccc gtgaggacgc actcccggaa ctcttggaag
    39541 aatcggccct ggcggagcac gatcacccgg tccgaaagcc gcgtcagttc gggcaggtcc
    39601 gaggacacca ggagcacgga caccccccgg gccgagaggg cccggatgtg gtcgtagatc
    39661 tcctccttgg ccccgatgtc gatgcccacc gtcggctcgt cgatgaagat cagcttgagg
    39721 tcccggacca gccacttggc cagcacgacc ttctgctgat tccctcccga gaggttgccc
    39781 accagttgtt ccggccgggg gggcttcacg ttcaggagct cgataccccg ggccgcccgg
    39841 cgatcctcct ccttgacgct cacgatgccc cggggggcga tcaggtccag gttcgggatc
    39901 ccgatgttcc gggtgatgga ctgggtggcc aggatgccct ggaggcgacg ctcctcgggg
    39961 atgaacccga tgccgccctc gatggacttc cgcaccgagg gcacccattc cttacccagg
    40021 tagcggatcg tgcccgcgtc gaaggagtcc gcgccgtaga gggcccggac cagctcggtg
    40081 cggccggagc ccaccaggcc cccgatcccc aggacctcgc cctcgtggag gtcgaaggac
    40141 aggtcctcga acttccccac cgaggtcaga ccccggacct ccagcaccac cttcccgccc
    40201 ttgggggtca gctcgtgctg gaccgccttc tcctccagct tgtggccgat catgaggcgg
    40261 atgatctcct cggggctgat ctcctccttg tccaggacct tcacgagctt gccgtcccgc
    40321 aggaccgtca cgcggtccga cagggcgtag acctcctcca tgcggtggga gatgtagagg
    40381 acgctgatcc cctcgctctt gagcagggcg atgatcttga agagcatctc gacctcggtc
    40441 ttggtgagcg aggaggtcgg ctcgtccatg atcatgatgc tcgccttgcc ggagagggcc
    40501 ttgaggatct ccaccagctg gcgctgggct atgctgatgt tgtcgatcag gtccgttggt
    40561 ttcaggggaa agttgtaccg gtcgatgagc tcccgggcct ggtcgttcat cttccgtacg
    40621 tcgatcagcc gggtgagggg ccgcagaggc tccttgccca ggaagatgtt ctgggccacc
    40681 gtcatacccg ggatcaggct gagctcctgg tagatgaccg cgatgcccag ggcctccgcg
    40741 tcccgccgga gatgctccat ggggaccacg gttccgtcgt acaccatttc cccggagtcg
    40801 cgccccgtgg ctccggtgac gatcttgatc agcgtggact tgcccgcgcc gttctccccc
    40861 accagggcgt ggacctcgcc gcggcggacc gagaagtcca cggcgtccag gaccacgtgg
    40921 ccggaatagg acttcgtgat ggagcggcac tccaggagga ggtccgcgcc gttctcgacc
    40981 ttcgtcatga cgccctccta cgccgcgtcc cggccgcgct cggccgagag ccgcttgagt
    41041 ttggcccggg cttccaggtg ctcccggagc ttcttctgcc agacgtccac gatgaccgtc
    41101 accacgatga tccccccgat ggcgatgggc tggatccagg gggagatgcc ggtcttcagg
    41161 atcccgttct tgatcagggc caggaagagg gacccgagca tcgtgcccca gacgtccccg
    41221 accccgccgg aaaaggcggt gcccccgatg accaccgagg cgatgacgtc catctcggac
    41281 ccgagcccga gctcgggcat ggcggtcatc atgcgggagg agatgaagac cgccgccagg
    41341 gaggcgcaga tcccggagaa catgtagacc ccgatggtcg tgcggtccgt gtcgatcccg
    41401 gtgagccggg ccgcggtcgg gttggctccc gtggcgtaga tattcgttcc cgtgcgggta
    41461 tgcttgagga agatttgggc gccgacgccc aggatcagga aggcgaccgt ggtccggtag
    41521 atcccgaaga ggccggaggt cccgaaccag aggtaggagt cgttctggat gtagacgttc
    41581 tggacgaagc ccagggagtc cttgacggcg atcaccaggg ccaggcccgc caggatgctt
    41641 cgggtggcca gggtcacgat gaactccggg agcttgtact tcgtgatgaa gaagccgttc
    41701 acgaggcccg tcagggcccc cacgaagagg gagaggaaga ccaggacggg gatgggcacc
    41761 ttcgtgtagt ggaggaagtt tatgcagacc atcgaggtca cggccacgac ccccccgatg
    41821 gacatgtcga tccccgcggt gatcatcacg aaggtgaacc ccacggcgac gatccccgtc
    41881 tgggcggctt cctggaggag cttgagcagg tttccggagc tcaggaagac cgggttgatc
    41941 acggtgatca gggcggaaag ggcgagcatc aggccgacca ggatcagcct tcgtacggca
    42001 tggtgggaca cggcgggcct ccgttttgat tgggaagcgt cgggaccgcc cgcgcccggc
    42061 cgcggcgaag cgccggcgcg ggcggggcgg acccgccagg gaagagaggt gctaccagcg
    42121 atcctcggcg gcgatctggc cgacggtctc cttggtggcg gcgaagggcg ggatctggat
    42181 gaccttggtc tccgcggcct tggaggggcc cagcttcttc tggaggttct ccatgaccat
    42241 ctcggcgatc aggctgccct gggcgaagga gttgttgtag gcggtgccgg cgatcttgcc
    42301 ctgctcgatc agggccaggg tgctcttctg gccgttggcg ccccagatct tgatctggtc
    42361 caggcggccg gcggcctcgg cggcgatcat cgcgccctcg gccatgttgt cgttcaccgc
    42421 gaacacgccg gcgagctcct tgttggcctg gaggaagccg ttcatgatcg tgatggcgat
    42481 ttccttcttc cagccctcgg cgctctgggc accgacgatc ttgatgttcg ggtagagctc
    42541 ggccatggcc tggcggaagc ccttctcccg gttgacgccc aggatggcgc ccggggggga
    42601 ctggatgatg accacgttgc ccttgccgcc cagctgcttg cccatctcct tgcccacggc
    42661 gtagccggcg gagatgtccg ccatctggac gctggcggtg tgcttctgga tggtggccag
    42721 gttcacggtg atgacgggga tgcccttggc ttccgcggcc ttgacggagg cggagagggc
    42781 ggcggcgtcc atggcctgca ggacgatggc gtcgtacttc tggttgatga cgtcgtccat
    42841 gtaggatacc tgaagctcgg ccttgagctg ggcgtcgaag gactggacct tgacgttcgg
    42901 gtacttgcgg aatacccgca tcatgccctc gagccaagcg ttggtgttgg gctggccgat
    42961 ggactggggg atgtacgcga ccttgtaggt ctgcgcgacg gcggggacga cgagcagcac
    43021 gcagagcagc gccgcgacga tggcggtctt cttcattctg tgcctcctgg gctggatggt
    43081 tcggatccga ttcgccgggg cgaaaatggt tgaagcatcg cgaatcgatc tcgatcaagc
    43141 ccaggataga tccataatgc tcatttggca agcattattt ttgcattttt tgatttttat
    43201 gttctaaata tgttttattt tagccaattc gatcagttta atgatcaatt cgcgttcaca
    43261 cgatgcccgc ctcaggccag gatcacctgg agaccccgtt cctcgagctc cttccggaag
    43321 gattccgggc attccgagtc cgtgaccacg atgtccacgt tggacagcgg ggaggacacg
    43381 aagtttgcca ccttgcccat cttggaggag tccgccagga ccaccacctt cccgtgggtg
    43441 tggtcgatca tggcctggtt gacgctgcat tcctggtaga cgctggtggt gagcccccga
    43501 tccagggaca gcccgttggt gcccaggaag gtgtggtccg cccagatgtc ccggatggcg
    43561 gacagggcga actcaccgac cagagactgg gactgttccc ggtactcgcc ccccaggatc
    43621 atgagctcca cccccgggga ggcgggcaca cccagggagg cgacgttgtt cgtgatcacc
    43681 ttggccctgc gtcccgccag ggcccggagg aagcagaggg tggtggagcc ggagttcatg
    43741 aacacgatct cgtcgtcctc caggagcctc accgcggccg cggcgatgcg gttcttggcc
    43801 tcgatgttga ggacgtcctt ctcgctcagg ttcttttcgg gtatggaacc ggactcctcc
    43861 tggggcacgg cgcccccgtg cgtccggatg agggagcctt cccggcagag atggtccagg
    43921 tcccgccgga tcgtgatggg ggagaccccc agggactccg agaggtcctc caccgtcgcc
    43981 aggcccctcg accggaccga ttccaggata tgcaagcgtc ttctgtacac cgccgcgttg
    44041 gatgccatga ttctccccgt tcgacccagc cggcattccg gaacgccccg ggtccgtatc
    44101 catatgtacc atacgaggcg cggctcggct agtatttttg atcgcttcgt cgtatatctt
    44161 gcgggaatcg atccaaacga tcgtatactg ttcacgggag gccccatgga caccgaaggg
    44221 atcatcgcga tcgacgcggg cacgacgagc atccgggcgg tcctcttcga ccgggccggg
    44281 cggcccgggg ccttcgccca ggcggaaaat ccccccgtct acttcgagga cggacgggtg
    44341 gagcaggagg cctccacgtg gagggaagcc ctgttccgga tcctgtccga ggtctccgcg
    44401 gccgcgggat cctacgggat ccgcgtcgag gcggtgacga tcaccgcgaa ccgttcctgc
    44461 gtgatccccg cggattccga gggccgggcc ctccacccgg ccctcatgtg gcaggacgtc
    44521 cgcacggacg ggatttgccg ggatctggct ccgcgctccc gggaggtgta cgaacggtcc
    44581 ggcctcccga tcacctcgat cttctcggcg gtcaagatgg cctggatccg gcgggagtgc
    44641 ccggagctct gggtccggac ggcccggatg ctgggagtcc aggattacct ggtcttcctg
    44701 ctcaccggga acttcgtcac gaaccgctcc cttgccagcc gcacgaacct cttcaacctc
    44761 gagtccgggg actgggatcc ggagctcctc gggatcttcg ggatcgagag gcgggtcctc
    44821 tgcgacctgg ttgaccccgg cgagacggcg ggatcccttg ctccggccgc cgcggccgct
    44881 tccggcctgt ccgcgggcat cccggtgatc accgcggggg gggaccagca gtgcgccgcc
    44941 ctgggcatgg gcctcctggc cgccggggac gtggtggcca acacgggcac gggggcctac
    45001 gtgatcgggc tggccgaccg gcccgtccgg gacccggaca tgcgcctgta ttgcaacgtc
    45061 tccgccctgc ccggtcgcta cgtcgcggag gcggcttcgc ccgcggcggg ggtggcctac
    45121 cgctggttcc gggaccggtt ctacgggggt cccgacgggt cggcggacgg attcgggacg
    45181 atcaacgccg aggtcgaggc gtccccgccg ggtgccaacg gggtggtcca tctgccccgc
    45241 ttcaagagca tgggcccggt tcgcgggatc ggaaccgggg gcccgggcgg ccgggcttct
    45301 ttccacggca tcgggcttgc gaccgcccgg ggggacctgg cccgggccgt gctggagggg
    45361 gtggccgggg acctggcgga gaacctggag ctgatcgagc ggtggacggg gccgaaggac
    45421 cgggtacgcg ccgcgggagg ccttacccgc ttcccggagt tcgaccgcat ccttgcgggg
    45481 ctctgcggcc gcccggtggt cctggccgcg gagagcgagg ccaccgcccg gggcgcctgg
    45541 atcagcgccg cggtccgcct ggggctgtat ccggacgcgg cttcggcctt cgccgcctcc
    45601 ggcgcgggcg ccggggaccg ggtcttcgag ccggatcccg cgcgccggga ggcgtatacg
    45661 aggctgaggg aggagcggaa gcggatggaa gtgaatgagt gaatagtgaa taggtgaata
    45721 ggacacgggg aatatgatat agccagacct gctccgtctc gattcgccat atagatatgc
    45781 cctacccatt cacttattca cccattcacc cgttcacttg tacacctccg gattcaggca
    45841 atccggcgct ttctggttct tgaccatggc gatcacgttc gtcgccgcct tcaaggccat
    45901 accgttccgg ctggatacgg tggcgctggc ggtgtggggg gtgatgacca cgttcggcag
    45961 ggcggccaga ccttccgcca gggcgggctc ccgttcgtag acgtccaggc cggcccccgc
    46021 gatgagcccg tcctccaggg cccggaccag gtcgtcctcg gccaggacgg gaccccgggc
    46081 ggtgttgacg aggtaggcgc tccgcttcat cttcttgaag gtgtcgtagg tgaagaggtg
    46141 cttggtctcg ggcatcaggg gcacgtggac gctgacgaag tcggactggg ccaggagctc
    46201 gtcgaaggac acgaagcggg ctccgatcgc cttctccaac gcttcgttgc ggcgggcgtc
    46261 ggtgtacagg accttcatcc ggaacccgac gctcttggcc gccatggccg ccccgatccg
    46321 cccggccccc acgatgccca gggtcgcccc ggtgacgtcc cccccgatga actgcatcgg
    46381 cccccagccc ttccaggccc ccgagcggtt caccccgtcg gactccacaa cccgccgggc
    46441 cgcggcgaag aggagggccc aggccatgtc cgcggtggcg tccgtgagga cgtccggggt
    46501 gttcgagagg gggatgcggc ggcgggtcgc ctccggcacg tccaggttgt cgaagcccac
    46561 ggcgaagttg gcgaagccct tgacccccgc ggcggcctcg aaggcctccg cgtccacctt
    46621 gtccgtcagg aggcagatca ccccgtcccg gcctttgatc gcgtccagga attccgggcg
    46681 ggtcaggggc cggtcgtggg ggctgacctc cacctcgcac tccttccgga gcaggtccag
    46741 tcccgcgtcg gggatccgcc gggtcacgta taccttgggt ttcgccatcg tcgtctcctc
    46801 caatgtgcag gcccggcgcg gatcgacggc cggccgggcc cgaaaagatt gcggtggttt
    46861 cggaaccgcc tcagtccgtg agtccgaaga cctcggtctc caggagcttc caggtttcca
    46921 ggtacccggg gccgcccatc cgcgactcga tggccttgga atccaggcgg tccaggagct
    46981 tctcgatgcg gctggacagc cgggcgttcg cctcgaaggt gcccgccacg tcccagcggg
    47041 tcgggctcgt gtccgaggtg aaccagccgt cgtacccgat gcggcgaaga taccacagga
    47101 actccacgta ctccaggtag tgcttggtgc cgcagaaata gtcccagtcc cacttgccgt
    47161 cgttgtcgtt gat
//
LOCUS       DAOTEI010000003        47019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_550_length_47019_cov_5.791862, whole genome shotgun sequence.
ACCESSION   DAOTEI010000003 DAOTEI010000000
VERSION     DAOTEI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 47019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47019
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_47019_cov_5.791862"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..1496)
                     /locus_tag="P5313_00490"
     CDS             complement(270..1496)
                     /locus_tag="P5313_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HRY78867.1"
                     /translation="MNRFLKTAAILLAVALIFAGCQKKPAEPAGPAKAAFHIGVVTGT
                     VSQSEDDLRGAEELIKRYGKVADGGMIQHITYPDNFMDQQETTISQIVALADDPMMKA
                     IVMNQSVPGAAEAFKRVRAKRPEILLFAGEPHEDPLVIEAAADLAINSDFISRGYTII
                     WAAKELGAKTFVHISFPRHMSYETLGLRRQIMEQACKDLGLKFAFETAPDPTSDVGQA
                     GAQQFILEKTPQWIQKYGKETAFFCTNDAHTEPLLKQLLAYGGMFVEADLPSPLMGYP
                     GALGLDLSAEAGNFPAILKKVEQAVIDKGGSGRFGTWAFSYGFTVTAGLGEFAKNVIE
                     GKTTKDSLQGLYDALAVYTPGAQWNGGYYIDANTGVRAKNHVLVYMDTYVMGKGYLPT
                     TKQKVDEKYYNIKFKK"
     gene            1779..3044
                     /locus_tag="P5313_00495"
     CDS             1779..3044
                     /locus_tag="P5313_00495"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY78868.1"
                     /translation="MRFLPKRFAEEFFLYSSQFTVFFILMLFSTPSIIALQPVALVVL
                     NGFLLLQITLLAFWGHKPLPRFLFSFITPAGYSLLQTITGWTFPVDMASFFLWAVSFY
                     VGLLQAWSGAARRSWPKRVLEAFLSFGTVLTFIFFYFYLDLRISLAMRFNAGDIPYEG
                     YIWGLSISSFLPAFVTFIKSPQHTFFAFGAFVFGILQLGNKVKELSLRARLERIVGSV
                     PVPTQDLKDEPGRAEAEGKDAAVIYGDIWNFSALTGKVSAREAVEVLNRYYALWNVTV
                     TRHGGYLDQFVGDTAVAVFGLLGEKDGAERAVACAFELLQELPHLQEDLAAAGLPVIK
                     HVGIGVHWGRIMVGQLNNGTCPALSVFGEAVSIAARLDSLCREFKQDVLVSQAVYKQV
                     GIETQQHLQMAGEVLLRGKTQPSAVYGRK"
     gene            complement(3052..4764)
                     /locus_tag="P5313_00500"
     CDS             complement(3052..4764)
                     /locus_tag="P5313_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HRY78869.1"
                     /translation="MDSTTPWAFLDEYRGKMFDGEWPTLPEMFRITARRYPDRPCFTV
                     YSPDRVALTYAEALARIERCAAFLAGAGIRKGDKAAVTGKNAPEWAVAYLGILFAGAT
                     AVPIDYQLRNEEIENILRASDSRFLFVDEEKYDSFDPKRLDLKGRYSLAPGKPGYVYD
                     LAPAPGGVPKEPAREGDLAAILFTSGTTGVPKGVMLTHANFVADCYLAQANLHIFPTD
                     TFYALLPIHHSYTMLAVFIEALSVGAEIVFGQRMVTSQILKDLKEAKVTMFLGVPLLF
                     NKVLAGILKGIRAKGPVVYGIIRFLMALSGMIKKVFKVNPGKKLFGSVLDKASLRTIR
                     ICISGGGPLAPSVFRQYNQLGIDFVQGYGLTETSPILTLNPTDAYVETSVGRVIPRAE
                     LRILDPDPDGRGEIVVKGPMVMQGYYKNPEATAEVFTPDGWFRTGDVGYLDSGNYLYL
                     TGRAKNLIVTEGGKNVYPEEIENRFQLYTEVEQVLIRGYVQDRKAKIEGIEALIYPSK
                     EYFEGPGARDGIDSGDVEARIRRVVDEVNSKMLPYQRISKVTLLEEPLEMTTTKKVKR
                     YSVA"
     gene            complement(4888..5445)
                     /locus_tag="P5313_00505"
     CDS             complement(4888..5445)
                     /locus_tag="P5313_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78870.1"
                     /translation="MSERFWRILGSAWFLAAAAALFAAFVPLVLVPYARKAAGYTPAG
                     GGFDTSFFYGPRQALDKAAAFDAPGRFSYISDRWTMDLAWPLVYGALFASAAAFALRR
                     ADSSPGAFALALRALVLAGPLLDYGENIAATVLVASVPARPLGWALAASALTPAKWIA
                     IAAAGAGILGLYLALAVRTLSGRRP"
     gene            5597..6337
                     /locus_tag="P5313_00510"
     CDS             5597..6337
                     /locus_tag="P5313_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78871.1"
                     /translation="MSRSRRPMRYLPALPAAAILLAALSSCAAGLSLAVNPDGSGTLA
                     VDARIPDAAAARLRSYAAAGTPGSAPTAPLFDTAAVRRETARRGLAAVSVETPTPNRF
                     RGSFSFRSLAELAADPELAESGILRVGSKDGVTALSFRLSRDNARSLPLLFPGLDPYV
                     LEALSPPALDPYPVSASEYREMLQALLGTAALGELDSAEIRIQVRAPDPVLRHAGGAV
                     SDRTFTAVLHLMDILVLEKPMEFSVSWR"
     gene            complement(6368..8266)
                     /locus_tag="P5313_00515"
     CDS             complement(6368..8266)
                     /locus_tag="P5313_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY78872.1"
                     /translation="MTPDAGPLKTLLLVEDEALIALAERRTLERYGFRVRTAADGEGA
                     VAAASEQDVDLVLMDINLGAGIDGTEAAKRILAARDLPLIFLSSHTEREVVERTEGIT
                     SYGYVVKNSDENVLVASIKMAFRLFEAKVQARAKEERITLLGRLLDAAPDSITIHDES
                     GAFLYANRRTFALHGYSEDEFMALNLHDLDVPESEVLIEERIRKIRDAGEATFEVTHR
                     RKDGTLLPLEVTVQTADWGGRFAMLSIARDRTERVRADREIRRSEARLASLLRINQYP
                     ARDIQDLLDYALDEAILLTESRIGYIYLYDEDRKLFTLNTWSKEVLHQCTVLEKQTVY
                     ELDKTGIWGEAVRQRKPILVNEYAAPSPLKKGLPEGHAPLRRFLTVPVFDEGRIIAVA
                     GVANKEAEYDESDVRQLTLMMDAVWKTVRRRRAEDALERQLREKETLLREVHHRIKNN
                     LASIGGVLRLQLDSVPDDASRTAILDAAARVESMRVVYDKLLVSGDYREVSVREYLEG
                     LLAAILDLFPDTGRIRIEPDIQEFELGTRTVFPLGTIVNELITNSMKHAFPGRDRGSL
                     GIRVAREGNEIVLEVKDDGRGLPEGFDPESSTGFGLQLVRMLAEQLNGSFSIRGGEGT
                     TGVVRFKA"
     gene            complement(8345..9181)
                     /locus_tag="P5313_00520"
     CDS             complement(8345..9181)
                     /locus_tag="P5313_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78873.1"
                     /translation="MPIARKSLPALMFLVSALSLSAMGTAEELRFPVRQSADGELTVI
                     STAGHKVERMSLPAFSPDGYRFSWQGSFYELATYPSGRFAVLSLDKYLKGRTLGHTVF
                     LKGRSDPEKPIPEWVTDERLLLTGLPQYSGFLALRYSRASMASTKKLTVERVLRDTIL
                     PFVVSTLENPSDSDRATAAILADDLARSLSSGSRLSTYWIEILRRAPNAAPKDTDFVS
                     TRKIYALVEVPIEGCLEAIAELQRSAGAGDAPEYASPSGVGMLEALSAFLRKETGIGS
                     TP"
     gene            9295..10146
                     /locus_tag="P5313_00525"
     CDS             9295..10146
                     /locus_tag="P5313_00525"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRY78874.1"
                     /translation="MKGVIVAAGYGTRFLPVTKTVPKEMLPLLTKPSIAFVVEEFAAS
                     GIRDILILTSRRKKALEDFFDRDPELESVFKAEGAAGKLERIAPPAVNVSFVRQPEMR
                     GTGHALLQAAPWLGGDPCVVAYPDDLHFGSPPLARQLMDVYERTGCSVLSGLHEPGDV
                     SRYGVIDPAPDGVHVRGFVEKPAPGTEPSREISLGRYLYTPEFFEILAEGWARHTGGE
                     YYHAYALDRLIAAGKVAWAPVTGTRLDTGDPAGFLDALLRFAWEDPGYRSVLESFIRE
                     KTGKPAL"
     gene            10169..10477
                     /locus_tag="P5313_00530"
     CDS             10169..10477
                     /locus_tag="P5313_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRY78875.1"
                     /translation="MKVVLVRIQVKPGAEEAFLAATLENARASNREPGVARFDVLRDA
                     GDPSRFVLVEAYRDEEAPARHKETAHYLRWKEIAEPLMAGERTREFYEGAYVPERDLR
                     "
     gene            10474..11682
                     /locus_tag="P5313_00535"
     CDS             10474..11682
                     /locus_tag="P5313_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRY78876.1"
                     /translation="MTWGFGGPGEILFGEGAVRELGKRAAALGTRAFVVTGRDPGRAA
                     AALDSLQAAGLEVAVFPAPGEPTFTVLRSALAEARKYAGPPAEGAHDASDPAAPPPGL
                     VVVGFGGGSALDLAKGLGILLSNPGDPLDYAEVIGAGRPFTAPSVPVIAVPTTAGTGS
                     EATKNAVFAEPSRGVKVSLRSPHMYPRLALVDPGLCLGLPSEVTAFTGLDALTQVLEP
                     FVCLKANPATDALCAAALAETAPALRRAFRDGGDLAARSILSRVSLFSGLALGNAGLG
                     AMHGLAAPLGGRFPVPHGAACAALLAPAAAVNIRALRNRDSGGPALDRYARAARLVTG
                     NAAAGPEDLPAALADLAADLGIPGLSVWGVREADLPGIAADAGTSSSMKANPVALSAP
                     ETEGILRAAL"
     gene            11810..12598
                     /locus_tag="P5313_00540"
     CDS             11810..12598
                     /locus_tag="P5313_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRY78877.1"
                     /translation="MNEEKGAGNANPRIARVVGVASGKGGVGKSTIAVLLAQALAAKG
                     SRVGILDADITGPSVPRLLGVDSFRAEADDGKLVPIKSDDGIEVMSINFLIEEETRPV
                     IWRGPLLSQAVKQFWTDTAWGDLDWLIVDLPPGTSDVMLTALQSLPFDGLLFAATPQD
                     FVSMIVAKAVHMAGQTDTRTLGLVLNMVSMVCPHCGKEIPLFTGGGTDDPDLGIPVLA
                     RMPWRADLAQKRHLLWDGLSEEVRRTASDLAEGVAGTLARAEVR"
     gene            12600..13277
                     /locus_tag="P5313_00545"
     CDS             12600..13277
                     /locus_tag="P5313_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRY78878.1"
                     /translation="MPRPVSERRVRGWPRSRAFKPAGIPLRELETVILTLDELESVRL
                     ADLEGLYQEAAAARMGVSRQTFGRIVETARRKIADALVNGKALEIDGGIVQMAETDMN
                     EYRIAVPTRDGMVDPHFGSCRGFTLFDVKDGTVVKEEYFPAPEGAGCKSAAASTLAKA
                     GVRVMLAGGMGEGAVRVMASFGITPVRGVTGPVRPVAEAWAAGKLSDSGEVCSHDHEG
                     HDCSHHS"
     gene            13386..14999
                     /locus_tag="P5313_00550"
     CDS             13386..14999
                     /locus_tag="P5313_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRY78879.1"
                     /translation="MKSSPSPTIKPKRARRPGRPAGARIFAAVLAAALLASCAGMSGS
                     AYERSAESDSSAAAVAAQRKKDSPPPSQPRTPPAPESGTSGFLFFGPPPPPPPRGWIT
                     VRGLPSGATLYVDDRPYSGGSAEVEAGDRFVEVRAFGYEPWSARVTVRIGESVELEAG
                     IFPAVFRLTELEAEPDTFDPGAPGALGRTRLEWTVTAPGRGDLEIRDTQGRTVFAARD
                     IELDSAYGDLAWDGRDGSGRSAAPGVYYARLSAAGRDGSASSLETSVEVSPEAAPQRF
                     SGLHGGFSGALFAPDARVLPEGSFQAAAGTYLLSTPQDADSAARMPAFAGIRAGGLFG
                     GRSELVASGMLVPYFGFSGADPSWGSLMVSVKTTLAAGPHGAAAFLASASVASFLDEA
                     SSGFPPAWDGPARYPGLGAGLVLEASTRTSRVFASLHAHASTYYPDWDGLEWDTPGLF
                     AWAYGRAGAEALISGVFGGDLALALSAATRTEPFGGARGFRPPLSVGAEAHWYAPESG
                     LVLSAYAAGEWAAFKSWYYAGGFGMGFAF"
     gene            complement(15184..15828)
                     /locus_tag="P5313_00555"
     CDS             complement(15184..15828)
                     /locus_tag="P5313_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRY78880.1"
                     /translation="MKKYLVLAGNIGAGKSTLVGLLAKRLGFMPYYEPVAENPYLTDF
                     YADMGRWALQSQLFFLTHRVKTHRALMDDPCSVVQDRSLYEDAEVFARNLREQGALSG
                     RDWSTYRELYTTVADLLPPPDLVIYLRASVPTLKKRIAKRGRDFEASIPDEYLAGLNR
                     LYEEWIGGFDRAPVLVVPGDRLDFVEESADFRAIASTIAERLRDKQGALFPYGM"
     gene            complement(15825..16874)
                     /gene="trpS"
                     /locus_tag="P5313_00560"
     CDS             complement(15825..16874)
                     /gene="trpS"
                     /locus_tag="P5313_00560"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRY78881.1"
                     /translation="MEHKRILTGDRPTGKLHLGHYVGSLANRVRLQDEYDCFFIIADL
                     HTLTTKPEKENIEGLADNVRSAVLDYLACGIDPARSVIYLQSAVPEVVELSLFFQNLV
                     TVPRLSRIPSLKEMARGAGLDEMPFGLLGYPVLQAADILLPRAHLVPVGKDNVAHVEL
                     TREIARRFNNLYGEVFPVPDVMEGEFGSLVGLDGNAKMSKSLNNAIFLSDDEKTVHKR
                     VMSMYTDPKRVSADVPGTVEGNPVFQYHDAFNPDKAEVEDLKARYRAGKVGDVEVKEK
                     LAKALNAFLDPLRERRAKYEAQSGLADEVVFDGTLRMREEARATLTAAKKAMGLSAVW
                     NRISRKAEDVKKKRG"
     gene            complement(16928..18325)
                     /locus_tag="P5313_00565"
     CDS             complement(16928..18325)
                     /locus_tag="P5313_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY78882.1"
                     /translation="MKDAESRDRESLLQAIFESTPDIITVSTLEDGTFLEVNPAFVQL
                     TGYTREEVLGKSSLSLGIWADPGERERFAGRLGRGEPVRGFEARLRSKSGEERRYQLS
                     ASPLVAGGRPALVMICRDITAAAEAAAVERKSAFLLERAEEMAKIGSWEFDYATKVVT
                     GSEGAARIYGVPRENLTVGDIETIPLPDYRPLLNKARDDHILRGLPYDVEFKICRRSD
                     GRILDLRSRALWDGAKKRLFGIIRDVTDEKRAEEGLRKAIAERDLLIQELFHRINNTL
                     QVVLSLLMLEAERDEDRRVDDLVSRMRRRVAAIASVHRSLYTARDVSRISLLDFLSRF
                     LETSSRIPGGPAAELFLDADGVDLNIEAAVPCGILLAELVDNAMVHGRVPGRPGRVSV
                     TARRLGDGRVELQVADDGPGLEVDLRDIPRPWLGLRLVKMLAEGQLGGTLQIRNSPGL
                     ECVVVFDDDRLGGSA"
     gene            complement(18546..19916)
                     /locus_tag="P5313_00570"
     CDS             complement(18546..19916)
                     /locus_tag="P5313_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY78883.1"
                     /translation="MKVLRKCSILALAGAVLFLSGCGARPGPSSSPVPEAARGAQAPE
                     TPPSPGPSGAAAGTGSAEAGQSSGTPGSPATAPGAAAPGAAAPAVPGQTSPGAPAPET
                     ARKAPRRIGELVYAEGTVTLHRGGSAPRPADIGQVVENFDVLQTGPKSRATLELDPGS
                     PGGAEIRLAENTAFYYETALSDDEARRTVLQLLAGSVAVKVEKLADGQFKVGTDSAVL
                     GVRGTTFIVNTIPDGALLVTCAEGRVEVTGGSGERGYAQPGRIVSQKAGESIREDLVA
                     LETLADFRVAWVDDGQKSLEARAPRVIAGLAGRYTEALPAFEAAYAKLKAQGSVLDAW
                     AVAREAGGEPKFTDWIPEKKAVAPVLFDCLAALFKLERPLFRLQELSALHDRGVGAGT
                     LPDGRTTAAFFGAYRAEAPGVADKLAYVRRSLLLFKWASAGSPLGQFFGSKAEALGSG
                     GLFLED"
     gene            20074..20961
                     /locus_tag="P5313_00575"
     CDS             20074..20961
                     /locus_tag="P5313_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78884.1"
                     /translation="MKKYLVPANAVAYAVAITAAIAVSIALAGCVSAPPEKAPEPRRK
                     ERVETVRVPVLLKESSYFADGVLDRTATYTYDTTLKFLLEKRVQEPSRTEPTERIVYE
                     YTGTAPASISVYDFEGKLRTRTDFKVDAAGRVLQETVSDPRGTPQSQVRYEYDPAGLR
                     TSRRVYDGAGALLAISEYSYTAGRLAVVILKDSAERPAGRVEHEYDPEGRLVVRVGFD
                     PSGAVAQRERFAYAGGALTEERLERGDGRVERRTVHEAGPGGAPAKSTVYDGSGRIRD
                     VRVYEYGFRSEERTVVYYE"
     gene            20976..22151
                     /locus_tag="P5313_00580"
     CDS             20976..22151
                     /locus_tag="P5313_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78885.1"
                     /translation="MKRSPKILAVLALACLTALASAADEMTFIYRRIYQQAETLRQKE
                     AAALAVVSRDDPGTASVLEYILGDLLRSGPEVAKGSQDRQVWNNLVRTTVGALGTARY
                     VEAAPLAWDAFNQSEDPLVRAEALMALGNMRALEYAERIALVLRNLNFEPVQDREAGE
                     KTAFGAILALEKLRDPVGFAPVFFASEGWYTRRVRDQALRSLPLLAEDPTEPVRRLIL
                     EESLDRKLRAFDLHAASKAPEERKIEIARLALQRGIEIKARDRAEGTKLRDLRVRGLR
                     SLVALKARGGASTDWIREAFRLGETDERLLALQALGTDASDPSARALAEILLDLDAQQ
                     RAGIADEVRNRIAAAAIQYAGATRNRLVRPALTAVSLNDTWSNSLIRAAQDALKELP"
     gene            22148..23278
                     /locus_tag="P5313_00585"
     CDS             22148..23278
                     /locus_tag="P5313_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRY78886.1"
                     /translation="MNDDAKTFAARRGRIAEWLKERGIAAAVFEDREGRRDPTVRYLS
                     GQPGDSLLLVTAAGRGVLVSWDVNMARKLSRVDEILAYGDFERKPVRAVLGVLAREGI
                     RPGSQVELPSTLAYPEYIRYAEEGADFDFLCREDGADEFARGLRAVKDESEIAVYRRA
                     SAVTDELVEALEAGVRKGTISTESDAALFLERESRARGCEGMGFETLAAGPARSFGIH
                     AFPTYTSGAFGTAGLSILDFGVCLEGYTTDVTLTFVSGRPSALQERMIALVVEAYKEA
                     AGMLRPGVPTLEVARRVDEIFAAGGFKMPHALGHGIGLEAHEAPAMRNRDDNPWVLAP
                     GHIVTLEPGLYDPEAGGVRLENDFLVLPGGAEALTRSRIVRL"
     gene            23275..24372
                     /locus_tag="P5313_00590"
     CDS             23275..24372
                     /locus_tag="P5313_00590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY78887.1"
                     /translation="MNPRLREGHPRIVQAGPILAIHKTLFVDRPLLSLAGAAAVYVFI
                     VLGFGRSLEISSNYFVLLPVLAAALGYGLAGGLVAGALGLPANLLLFNLLGRPEFSPA
                     SKPIAELSGLLVGGASGYLAEYFRQLQSEIARRIRTEESLRRALEDKQVLLLELNHRV
                     KNNLNVIKSLIQLQRNRSEAPEFREASDRLLARVFAIARAHDRMFGEDGPPVLDPVEY
                     LQDILAVYDAEAGIGPRIEANLRPDGLRLPAETAVPLGLILNEAVANAWKYGSSREEG
                     TIRVSLARTGAGCVLEVRDSGPGFDPDAVGRPDRPQGLGVKIIRSLAARLGGRVSWSR
                     EGGPRFRLEFPAAADPGKTGSVVADPGPGRL"
     gene            24390..25208
                     /locus_tag="P5313_00595"
     CDS             24390..25208
                     /locus_tag="P5313_00595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY78888.1"
                     /translation="MKTTSAAYIMENDQESRRLELKTDVRVVRDQARRAGLEPGMRVL
                     DAVCGPGITTAALAEEAGEGGSAVGIDFSEDRIARAREAHGGARTEFLVRDILEPLEG
                     LGTFDFIWMRFALEYYRAESFDIVRNLASVLREGGILCLVDLDYNCLSHHGMSGRLEK
                     ALRHVVDTLEARANFDPYAGRKLYSHLYRLGFRDIQVEAGAHHLIYGPLREVDEYNWT
                     RKMDVLGDRISGDLPGYSGLDEFRKDFLAFFRDPGRFTYTPVLSAWGRKPPAGA"
     gene            complement(25245..27347)
                     /locus_tag="P5313_00600"
     CDS             complement(25245..27347)
                     /locus_tag="P5313_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRY78889.1"
                     /translation="MNKRNDGPVRTDDEPLYNSKIISTYLRLLKRKYPFVDSAALLAR
                     AGMAPHQAEDEGHWFTQAQVDLFYEGVVKATGESGIAREAGRYSASPEGLGRFYHYVL
                     GLSNPGKAFELISNLAGRLTRSTLFESRTLSRKQIELSVTPREGVREKPYQCENRIGY
                     FEAILSGYNSRKPRIEHPECVFKGGKACRYRISWDDSFSVVLRMARLGLLAACVPILA
                     AAAGYRGGERFLETAAILSAAVLALSLAKEQVQFHELNAVIEHQRLTTERSFEDFERV
                     YNNALMGKEIGHVLSASTDVDAMLARVMEILSQRGDYDRGIILLADEDKRNLVFRAGF
                     GYTEDLYREIRATRFSLYKPDSRGVFVICYRDRKPFLVNDVSTIESEISPHSLEFLRR
                     LGSKSFICCPILFEDQCLGVLAVDNLQSKRPLLESDINLLMGIAPEIGLTLNSAFFAV
                     ERERQFRSILRTLAASIDARDSLTAGHSERVTEYAIAICEEMNMPAEFTEVIRVAAQL
                     HDYGKIGIKDSILKKSGPLTGKQREEIKTHVVKTQDILSRINFSGTYQQVPFIAGSHH
                     ERLDGTGYPRGLRGEEIPLGARILAVADFFEAITAKRHYHEPLRFEEAVETLKREGGR
                     HLDEAVIEALLRAIVKGRTAVPGAPAVPGGGAGPAPRGAVPGGAVARPAAPVPPGSGL
                     VAAARGAPVVPGPEAAAL"
     gene            complement(27313..29493)
                     /locus_tag="P5313_00605"
     CDS             complement(27313..29493)
                     /locus_tag="P5313_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY78890.1"
                     /translation="MNDYSRNRRWILTGSPTIPPRPAVHDDGGLGSSAPFLPPGLPAK
                     PAVQVGAASAEDLRGGRRIKHRDLANLLNRIHFKEGSVFVRFERRDGVGSFSLKALPL
                     PSDGRTLSCRWASRGPEPEKLAAYSCSGLLISDGTLYTSVPAEAAGMDEAGIELRLPE
                     PGTQESVRGQERYPVAGVRAKVLQSGVTWEGNLEDFNGVSLGVILDDGPSPSRNWLNP
                     SQPVTLLLFRDGILLFSGECAFLARDRGAPGKRMALSPTRTNIQRFPPREHRSHRREL
                     SPQPVVRFTHPLSGRTAFLRAENVSGMGVCVEEFFEQSSLFPGLILEDLTVELAGSVL
                     AACRAQVLYVNVLEKPEGRRTARYGLAFLDLDARDQVRLSSAVHASIDGRLSVCGRVE
                     MEELWQFFFDSGFLYPSKYLSLQTHKDEFCRTYRKLYQDSPSIARHFFFRDKGRIFGH
                     MSMLRFYPDSWLIQHHAASRDGYGAAGVSVLDEAGRFTNDFHLHPGARMDYLMCYYRP
                     ENRFPARVFGNVAKDIADPKGSSVDIFGYLRLPAPPEGAPGGFQLFPARPEDFRELRR
                     WYEARSGGLTLDALALTKSDGSDAELSGEYRREGFRRDRQVYSLRCGGALAAVIQVTV
                     SDLGLNLSSLTSCLHVWVLDPERLPAGTLFSALDALRGAYDDETPPVLVYPEEYLDRY
                     SVPCEKKYALWVLNTAYSDAYFESVRGTFRRSCREQEERRAGSD"
     gene            29692..30591
                     /locus_tag="P5313_00610"
     CDS             29692..30591
                     /locus_tag="P5313_00610"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRY78891.1"
                     /translation="MERLRGAEGPVSGEYLARDLGVSRVALWKRIEALRAWGYRIESS
                     RRGYALVRDDGLAPADLPPPVRVLSEAGSTMDEARDWGSAGAPSGAAVLALRQSAGRG
                     RGGRRWESPDGGLYLSLVLRSALPAACAGALVLEAARSLLDALATAGIRGVSFRWPND
                     LVAGPRKLGGILAESFGGLDRPEFYVLGIGLNAAPVEVSGRAAAGLADLAPGLPRRRD
                     LALGLSESLRAWCERPDTDPRRWAGLKPDPRRPARAELWDGRILGIRPRGYTTRGELA
                     REGDGPALAFGECRKMTYEGEEP"
     gene            30588..31145
                     /locus_tag="P5313_00615"
     CDS             30588..31145
                     /locus_tag="P5313_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRY78892.1"
                     /translation="MKNRPWNLAASALFAALISAGALFSLPLPPPLPPVSPAVFFVLL
                     AGLVLGPAGAGASAGIYLLLGSLGLPVFANGAGGLGHFLGPTGGFLAGYLAAALVTGA
                     LADRKRWGAGRSFLAAVAGVLALYALGLPWMRQVLAAKLATLAAAAALMLPYFIGDLV
                     KAAAAALLVRALKPVLERHFPREDL"
     gene            31142..31888
                     /locus_tag="P5313_00620"
     CDS             31142..31888
                     /locus_tag="P5313_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY78893.1"
                     /translation="MRLEARGLVRIFPDGTRALDGADLELRTPCFMVLSGPNGSGKTV
                     LARHFVGLEKPQAGAVLVDGVPVERCLAETRRRVGFVFQEPEHQILGITAGEDVEFGI
                     RVRVPDRGERRRRALRALRDAGLAGSEDRLASSLSGGQKRRLAVASALVSDPELVILD
                     EPFNGLDPAGASGLLRMLLSLRDRGVGILLVTHDLEKCLAHADRLAVLDSGRVAADGP
                     PAELWDRLPSLGLRRPPGGSGRLEEMTWLD"
     gene            31876..32556
                     /locus_tag="P5313_00625"
     CDS             31876..32556
                     /locus_tag="P5313_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HRY78894.1"
                     /translation="MAGLNGRPAAFPSDYRARLALYLALQAAVFLAPGPTILLPAAVV
                     AVLGARAGVDWGRWLVRLGPLLGMILLPALAGLPEAWPVRAEGAAFLAAWSPGLRRSL
                     AFLLVLASAEWLSRTTRVDEIRDTLEGLLGPLGPRGRRAALAAALTLGFLPWARYELA
                     RADEAARLRGSDPRRGPLRHLAVLGIPLTARLLEKARLSSEALALRDADPDPGASNLA
                     RSPRRFYS"
     gene            32573..33469
                     /locus_tag="P5313_00630"
     CDS             32573..33469
                     /locus_tag="P5313_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78895.1"
                     /translation="MRISRFLAAGILLLASASAAGQTRRVAVLPFENDGLAAGDVRGL
                     TLFFETALQNTKLCEIVEQTKVEEILKAHQFVLADFNDPAKAVAIGKLVPANTIALGA
                     AGKLGGRPYLNVKLIDLVSGTVVAARNVTAASVEALTSELNALAAGIMGSPSSSLASP
                     PETSRPGPPVPLTAVPRVGRSQLSMFESGPGIPPPESRIYAVTFFTAYTRFINWDLFL
                     EFSAPVSRSMTLPVDATILRSNGTVLTVQSLTANLESGWNWFRMWNGWGAPNPYNWAP
                     DTYTLRVTLGGVPVATTTFVVR"
     gene            33610..33978
                     /locus_tag="P5313_00635"
     CDS             33610..33978
                     /locus_tag="P5313_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78896.1"
                     /translation="MKRFALTALILALALAPAAAQGIDLGNFPLGSWLDANYDAVWEF
                     SSGNIRILSPAGDVLFDFGAAGVQNFKVGAGGDGPFITFACEAAGRTYKITKPLTKSS
                     AVLEIQRPNLADYKVEMPKK"
     gene            complement(33951..34304)
                     /locus_tag="P5313_00640"
     CDS             complement(33951..34304)
                     /locus_tag="P5313_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78897.1"
                     /translation="MDAAAVGFFAVGLADCFGAGLAVFEAALLTGGAAAGFLPVFGFA
                     EAGGAFEGFLVAAMTFSFVFRMDRLGRPGREPPRRRGNRITRSTRAQRGAGKSGGPLL
                     GGPPAPAYFFGISTL"
     gene            34336..35682
                     /locus_tag="P5313_00645"
     CDS             34336..35682
                     /locus_tag="P5313_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78898.1"
                     /translation="MIPIPGTAPDVPAQIVAFLEKFDALKAGKTWAVKAAALIEKIPA
                     ALFGEFEDALDLETPLSTGKIPLHLIDPKTAKTAAAKTGKGTIPAPRPSPKTFGTAIL
                     GNKIGREELVELVERLRWCLCCCHCWPCRPWPFCRICRWRLVKVSSTADSGWVYHLNF
                     KNASAAIADRVAEISCPSGGSSGGPWAPQVWNASTPAAEAPVGYAEAGHDHDGVYSAT
                     GHNHDAAYAPKQKSGADIKYAEIDAPVGQTVKLRDKATSPSPADVTVSLSGHNHDTAY
                     AAAGHNHDTAYAAAGHNHNTVYAAAGHNHDSSYAAAGHNHDSAYAAASHDNTSHTKKY
                     FSRTNLIMKANPDTTLDAGHYWVKIQLSLPVGFAPGTSNWAECVSGVCMDPTMAAAPL
                     DLQAVSPTIITELEGGVNVPKLKFRLTDSGGTDYRKNNGIWAGKLVVVTVAAPVTD"
     gene            complement(35689..36939)
                     /locus_tag="P5313_00650"
     CDS             complement(35689..36939)
                     /locus_tag="P5313_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HRY78899.1"
                     /translation="MRTRSIASGLAALALLAAALSCQNINVETETLQPPVISNGEPDF
                     RYSMTPARGRSVTITDPNGGADIYYTLNGSTPTNTVSATNYQYTVPFNLAGTEDATTV
                     KARAFKPGLRESSVASRTFELAWKKMSATLTTARMGHGAVEVDGKIYIIGGFTDQSFG
                     LMTADCEVYDVGTGVVSDLGYDLNSARAAFGIAVLNRKIYVIGGTVNGVPDPDPVLVP
                     SASIEVFDTQNPGNWTTVTDELPVAIDNLCAAVSGGVIYIFPGDSGVGSLDTIYAYSP
                     GSDPAAVTLSSGLYSDRTYFQAVPIGAGILLVGGSMSTKDVGSFDPSGPSISDLTDLT
                     TSSYRFASAADLGNVYLFGGYVIGISAAVRKYSDSEGESQDFTMPAALDYPAAASSGG
                     YVYVSGGLVSMSSPPVNTIYRFTP"
     gene            37251..37604
                     /locus_tag="P5313_00655"
     CDS             37251..37604
                     /locus_tag="P5313_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78900.1"
                     /translation="MAASKPAKEPAAAAKPKTEKKPAEKKAAAPKPVAVKKPAAKPAA
                     EKKPAAKPAAKPAAAKKPAAKPAAKPAAAKKPAAKPAAKPAAAKKPVEKKAAAPKPAA
                     AKKPVAKPAAKPTGK"
     gene            37793..38722
                     /locus_tag="P5313_00660"
     CDS             37793..38722
                     /locus_tag="P5313_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRY78901.1"
                     /translation="MDRRDFLKTLGRGAGALGAAWLLGPASRVFAQAGGTPTPGAYPD
                     LVAVKGGSAASRVDAALAAAGGVSRYVRKGALVVIKPNMGWDVRPELAANTDPAVVKR
                     IVELCLAAGARKVVVFDHTCDSWRAAYANSGIERAARDAGAEAAPAHAESYYQAVANP
                     AARTLGQAKVHELVLQADVFINVPVLKNHGGAGMTCAMKNLMGAVWDRGYFHSRGLDA
                     CIAEAALLRRPDLNIVDAGKVMLSGGPRGSAASRYSEQRLLMLSSDIVAVDVASARTL
                     GPGPDAFEYIAKAAALGLGQPDLAKLAVRRIEL"
     gene            38719..40350
                     /locus_tag="P5313_00665"
     CDS             38719..40350
                     /locus_tag="P5313_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY78902.1"
                     /translation="MRIPNPARLASRIRGGLAALWLAAFCAGVALPATELPETAPLAA
                     FLARTQPFHALEKILLARAVPAAAGASILAVLTVLVGTLVLGRFHCSLTCPLGTCQDA
                     AARLASGLRSPGRGSPRPAHRRLRTAIPRWTVFGLAIAGLAAGIPAAAALLEPYTIFA
                     RGFQRSFGDLVALGLNGLAALGARIPILTVRWEPFAFALAVLPFGAVLSAAAFRSRLF
                     CGELCPAGTLLGTLNRVALLRVRLDRKACIDCGACARVCRTRCVDGRTLDASRCVQCL
                     DCVAACPTGALRYGPPRRAAGVDASPEFAPDPGRRSFLAEAGGAALGAAAGTALALAA
                     PGLAGTAGSPLVRIEPRGASGRLLAAPPGALSVDRFAGLCTACGTCIRACPSGVLKHS
                     TVEFGLGRPGVPYLDYGRAFCQYECAACPAACPSGALLDRDPDLKKREAIGKSALVLA
                     DCIVVSKGTRCGACAEHCPTGAVRIERVEGRPLPVPVLHAPTCIGCGACETVCPSTPR
                     KAMTVSGLAVHEIADPPRKEPPVPVEHPPEEGFAF"
     gene            complement(40347..41834)
                     /locus_tag="P5313_00670"
     CDS             complement(40347..41834)
                     /locus_tag="P5313_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY78903.1"
                     /translation="MSDELLIIGAGTAGLSAGVYAAASGYRVTVLEMAGTPGGLCATW
                     SRGGYTFDGSAAGLAGSGPGAPLYRMWKELGVIGRCPLRDPESFGSVRLSDGRTVTVW
                     TDIDRFEAHFLDNFPRDARAVRAFVGALRACLRFDMPLAGEPRGAGPSALSTLPVLLR
                     YGTLTLRRFLEGLSDPLAVEAFGNLVHFGGTDVPLLTVLLPLAYAHRRMTGIPRGGWI
                     SFARAIEARLGELGGRVLYGARVVRLKIESGKVRGVALADGREFPADRVLSAADGRFT
                     RSLLPGGSSDGAEFDPRRMSDQPVQVNLGVAEDWSEYPGPLTWLLPDAPSAAGRPQHR
                     LTVHHRCYDPASAPAGKSALMAFLESDYGFWKDLEADRARYEAEKRRCADIVVQAVQR
                     FRPGFAGKVEVTDVSTPRTRERYTGNYMGTMQARKPDAGMIRTLLRGAARYDDPGLDG
                     FWRAGQWVEAWGGITTAAQSGRNAIRAMCRRDGVPFAPGLRGDAA"
     gene            complement(41824..42492)
                     /locus_tag="P5313_00675"
     CDS             complement(41824..42492)
                     /locus_tag="P5313_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY78904.1"
                     /translation="MKYEPSTTRQSILDSAAALIRKAGSVRDLTVRRIIQAAGVNLNA
                     VNYHFGSKEALIREAVRAVIGEYYRERGILPGSTGPGLRKNLARICDFLFDEPVAARL
                     ALESEMESGGGGESLTGETLSALAAMLRSESPGLGEAEARFRVWSLLAFVHMTLVRPE
                     GAAEWLGADARDKKTRDRLLAKACDLLGIPDSGTGGAKLVKTERDARRREARREGKDG
                     SNER"
     gene            complement(42584..43153)
                     /locus_tag="P5313_00680"
     CDS             complement(42584..43153)
                     /locus_tag="P5313_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78905.1"
                     /translation="MNGRFGTAMAFLFGKEPRKPEVALVLALGLGGGVAAALAGSPWG
                     WSPVQRAVGFLLAADILGGVAANRTVSTDRFYARGGRPLRFSFLGIHILQPLLLWAAF
                     PEIGPRAPLALWGVSAVFATAVILADGKPWQDALGAGLAALGSAAGVLAFGRPDLAWF
                     PVLYCHKLVSSFAPVRIPGAVSKDRTIRD"
     gene            complement(43178..45124)
                     /locus_tag="P5313_00685"
     CDS             complement(43178..45124)
                     /locus_tag="P5313_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78906.1"
                     /translation="MDKNAKKPASNATGDKAARAAAKAEAKSVRSAAAAAKSAAKEAK
                     ANRGYVKAGPLLGLVLLAAGLAVFGIFFMNPLLESGAEAGLEAAFGARATVEGLRFRP
                     FRMRLSVGSVAVADAASPMTDLFRAGRLEFRLSPSAVLRGKVYIEEASAASVELGAPR
                     STSGALPGAAEPEPRQKRPAPEIPALVDWEAFDAQALLEREKAKLAAPAAYEAAGRSY
                     ARSVEEWKGRRETSSAAVEKARASSTAALAIDVKAIRTAGEAAQALAVVKAAAGDVQS
                     VGTEAKAVAEGIRKDTAALDRQAKEARDTADRDAAYLKSLVTPGSGAARAALEPALRS
                     LLTDKGETWLYYGGRFLEAASRLKGSGGGAAGADKAGGKPAFRGRDVVFPGAAFPRFR
                     LGLLTSSFKAGDSAWYVEVREVSTEPDLVPAPSSLKIEIRRGGSLVRADARADLRESA
                     GGSWEASLSAAHLPLDLGDSLAGAGLSGLTGILEGTSEVRGVRDGGFSASLEAEVRNP
                     VVAAPSGTLGKTAADALRDAGAVNLTAEYRVVPGGKDSFVLRTDLDRIIAGAVDAAGR
                     RYAAQASRELDAALRRYIDGELEGKLAPKKDFDSLFKAAEGDAASAASLEKSLDGKRA
                     EIEARSKALGADLLKGVTVPKIGP"
     gene            complement(45134..45673)
                     /locus_tag="P5313_00690"
     CDS             complement(45134..45673)
                     /locus_tag="P5313_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HRY78907.1"
                     /translation="MFLKLIARVIVTLSSNTNPVQIAAGVASGLLLALLPAGNLLWIL
                     LFLLLFLSKAHYGMALVSMAVFKLAAPAAAGPLDALGWTVLNAPVLRPAFTALYGMPV
                     APLTRFNNTLVTGGLLAGIVLWAPAFLAARAGVSAYRARIAPRIAGSKLVTALKKVPL
                     VSALAKAVGAAAGIAGSAE"
     gene            complement(45872..>47019)
                     /locus_tag="P5313_00695"
     CDS             complement(45872..>47019)
                     /locus_tag="P5313_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRY78908.1"
                     /translation="HAVDHFLLETGPYKGTKVCVDGPEYETAAGVGSNLYVYDPHGIL
                     EINFYCDTYGIDTISFGTMTSFLMECWELGVLNKERTDGIDLSWGNWKGACQILHDMA
                     EGKRFGVLAGRGVKFLADYFAKEYGADPQVMKDIALHGKGLEQSEYISKESLAQQGGY
                     YMTNKGPQHDEAWLIFMDMVNNRIPTFENKAEALHYFPMFRTWFGLQGLCKLPWNDVE
                     PADNAKWPEPNKVPEHVHNYIDLYTAITGEPLDWEGLILQSERVYNFQRTFNIRQGFG
                     HRKDDKPPYRAMGPVFEDEYEARAERYDKQMKEKIGIDPAGKTVAEKVKITREYRLKQ
                     YESLLDAVFKRRGWTPDGCPTPEHLKKIGMDLPEVLEVVKGRIEGRL"
BASE COUNT         7220 a        16450 c        15955 g         7394 t
ORIGIN      
        1 ggatcctgcc cctgcccagg gtgaaggaca cgtctttgag gaccgcggtc ccgtagaagt
       61 ccttggtgat gccctcgaaa ccccataagg gcgattcatt cgacataggg acaccttccg
      121 tgcggaatcg gccgggggga ccggccggga acggcgcggc gccgggggga aggcgcgcgc
      181 taaggccggc ccccgggtcg tccccgggaa ccggccgccc ggggtgtccc gcacgcggtg
      241 cgcgcgcggg ggccccgggg aagctcgggt tacttcttga acttgatgtt gtagtacttc
      301 tcgtcgacct tctgcttggt cgtgggcagg tagcccttgc ccatgacgta ggtgtccatg
      361 tagaccagga cgtggttctt ggcacggacc ccggtgttgg cgtcgatgta gtagccgccg
      421 ttccactggg cgccgggggt gtagaccgcc agggcgtcgt acagaccctg gaggctatcc
      481 ttggtggtct tgccctcgat cacgttcttg gcgaactcgc ccagacccgc ggtgaccgtg
      541 aagccgtagc tgaaggccca ggtgccgaag cggccggagc cgcccttgtc gatgacggcc
      601 tgctcgacct tcttgaggat ggcggggaag ttcccggcct cggcggacag gtccaggccc
      661 agggctcccg ggtaccccat caggggggag ggcaggtcgg cctcgacgaa cattccgccg
      721 taggcgagca gctgcttgag caggggctcg gtgtgggcgt cgttggtgca gaagaaggcg
      781 gtttccttgc cgtacttctg gatccactgg ggggtcttct cgaggatgaa ctgctgggcg
      841 cccgcctggc cgacgtcgga ggtcgggtcc ggggcggtct cgaaggcgaa cttcaggccc
      901 aggtccttac acgcctgctc catgatctgg cggcgcaggc ccagggtctc gtaggacatg
      961 tgccggggga aggagatgtg cacgaaggtc ttcgcgccca attccttggc ggcccagatg
     1021 atggtgtagc cgcgggagat gaagtcgctg ttgatggcca ggtccgcggc cgcctcgatc
     1081 acgagggggt cctcatgggg ctcgccggcg aagagcagga tctcgggccg cttggcgcgg
     1141 acgcgcttga aggcttccgc ggcgccgggc accgactggt tcatcacgat ggccttcatc
     1201 atgggatcgt ccgccagggc gacgatctgg gagatcgtgg tctcctgctg gtccatgaag
     1261 ttgtcgggat acgtgatgtg ctggatcatc ccgccgtcgg cgaccttgcc gtaccgcttg
     1321 atcagctctt ccgctccgcg caggtcgtcc tcggactggg agaccgtacc ggtcaccacg
     1381 ccgatatgga acgcggcctt ggcgggacct gcgggctcgg cgggcttctt ctggcagccc
     1441 gcgaagatca gggcgaccgc gagcaggatc gcggcggtct tcaggaaacg attcataatg
     1501 cctccttcgc ctcgatgtgg cgatacccta gattctactc cgattgaaaa cttggtcaat
     1561 acagtcccgt cgtttctata tatagaatta tgcccgcgat ttccggcctc cggggcggat
     1621 ccctcccggg tcgcatccgt ctcccgcatt ccaggcctct ccgagcccgg cccgccatcg
     1681 cgcgcccggc agggtttcct tttcccgggg aatcctttat catggccctt cggacgctcc
     1741 ggcgaaccgc gggtccaaat ccagacgtcg agggttgcat gcggtttctt cccaagcgct
     1801 tcgcggaaga gtttttcctc tactccagcc agttcaccgt cttcttcatc ctgatgctct
     1861 tctcgacccc ctccatcatc gccctccagc ccgtggccct ggtcgtcctg aacgggttcc
     1921 tcctgctgca gatcaccctc ctggccttct ggggccacaa gcccctgccc cgcttcctgt
     1981 tctccttcat aacccccgcg ggctactccc tgctccagac catcacgggc tggaccttcc
     2041 ccgtggacat ggccagcttc ttcctgtggg cggtctcctt ctacgtgggg ctcctccagg
     2101 cctggagcgg ggcggcccgg aggtcctggc ccaagcgggt cctggaggcc ttcctctcct
     2161 tcggcacggt cctcaccttc atctttttct acttctacct ggacctccgt atctccctgg
     2221 ccatgcgctt caacgccggg gacatcccct acgagggcta tatctggggc ctttccatct
     2281 cgagtttcct gcccgccttc gtgaccttca tcaagtcgcc ccagcacacc ttcttcgcct
     2341 tcggggcctt cgtcttcggg atcctccagc tggggaacaa ggtgaaggag ctgtccctgc
     2401 gggcccggct ggagcggatc gtcggctccg tgccggtacc gacccaggac ctgaaggacg
     2461 aaccggggcg cgccgaggcg gagggcaagg acgccgcggt catctacggg gacatctgga
     2521 acttctccgc cctgaccggc aaggtctccg cccgggaggc cgtggaggtg ctcaaccggt
     2581 actacgccct gtggaacgtg accgtcaccc gtcacggtgg atacctggac cagttcgtgg
     2641 gggataccgc ggtggcggtc ttcgggctcc tgggggagaa agacggggcc gagcgggccg
     2701 tggcctgcgc cttcgagctc ctccaggaac tgccccacct gcaggaggac ctggccgccg
     2761 cgggactgcc cgtcatcaag cacgtcggaa tcggcgtcca ctggggcagg atcatggtcg
     2821 ggcagctgaa caacgggacc tgcccggccc tgtccgtgtt cggggaggcg gtcagcatag
     2881 ccgcccgcct ggactccctg tgccgggagt tcaagcagga cgtcctggtc agccaggcgg
     2941 tctacaagca ggtgggcatc gagacccagc agcaccttca gatggccggg gaggtcctgc
     3001 tccggggcaa gacccagccc agcgccgtgt acggaaggaa gtagcggccc cctaggcgac
     3061 cgagtaccgc ttgaccttct tcgtggtggt catctccagg ggctcctcca gcagggtgac
     3121 cttggagatc ctctggtagg gcagcatctt cgagttaacc tcgtccacca ccctccggat
     3181 ccgcgcctcc acgtcccccg agtcgattcc gtcccgggcg ccggggccct cgaagtattc
     3241 cttgctcggg tagatgaggg cctcgatccc ctcgatcttg gccttcctgt cctggacgta
     3301 gccccggatc aggacctgct ccacctcggt atagagctgg aaccggttct cgatctcctc
     3361 gggatacacg ttcttgcccc cctcggtgac gatcaggttc ttggcgcgcc ccgtcagata
     3421 gaggtagttc ccggaatcca ggtagcccac gtccccggtg cggaaccagc cgtccggggt
     3481 gaagacctcc gcggtggcct cgggattttt gtagtaaccc tgcatgacca tgggcccctt
     3541 cacgacgatc tcgccccgac cgtcagggtc ggggtccagg atacggagct cggcccgggg
     3601 gatgacccgg cccacgctgg tttccacgta ggcgtccgtg gggttgaggg tcaggatggg
     3661 gctggtctcc gtgaggccgt agccctggac gaagtcgatg cccaactggt tgtactgccg
     3721 gaagacgctg ggcgccaggg gacctccccc ggagatgcag atgcggatgg tccgcagaga
     3781 ggccttgtcc aggactgaac cgaagagctt ctttcccggg ttcaccttga agaccttctt
     3841 gatcattccg gaaagggcca tcaggaaacg gatgatcccg tagacgacgg ggcccttggc
     3901 ccggatgccc ttcaagatcc ccgccaggac cttgttgaag aggaggggca cccccaggaa
     3961 catcgtgacc ttggcctcct tgaggtcctt gaggatctgg ctggtcacca tccgctgtcc
     4021 gaagacgatc tcggccccca cggacagggc ctcgatgaag accgccagca tcgtgtagga
     4081 gtggtggatg ggcaggaggg cgtagaaggt gtcggttggg aagatatgca ggttggcctg
     4141 ggccaggtag cagtccgcca cgaagttggc gtgggtcagc atgaccccct tcgggacccc
     4201 cgtggtgccg ctggtgaaga ggatggccgc caggtcccct tcccgggccg gctccttcgg
     4261 aacgcccccg ggggcgggcg ccaggtcgta gacgtagccc ggcttgcccg gagccaggga
     4321 gtaccgcccc ttcaggtcca gccgcttggg gtcgaaggag tcgtacttct cctcgtccac
     4381 gaacaggaac cttgaatccg aggcccggag gatgttctcg atctcctcgt tgcgaagctg
     4441 gtaatcgatg ggcaccgccg tggccccggc gaagaggatc cccaggtagg cgaccgccca
     4501 ttccggggcg ttcttgcccg tgaccgcggc cttgtccccc ttgcgaatgc cggcgccggc
     4561 caggaaggcc gcgcaccgct cgatccgggc cagggcctcg gcgtaggtca gggcgacccg
     4621 gtccggggag tagaccgtga agcagggccg gtccggatag cgccgggcgg tgatccggaa
     4681 catctccgga agggtcggcc attcgccgtc gaacatcttg ccgcggtatt catccaggaa
     4741 tgcccagggg gtcgtcgaat ccatggtttt cgctccgaat tagggatacc ggaagggttc
     4801 cgggcgcttg ggcgctgaga gttagacggt cccgaccgcg gaacggttgc ggtcgaatcc
     4861 cgccgctgct tcacaagcct cgggacacta ggggcggcgg ccgctcaggg tccggaccgc
     4921 caaggccagg tacagcccca ggatgcccgc ccccgccgcg gctatcgcga tccacttggc
     4981 cggggtcagg gccgaggccg ccagggccca gccgaggggg cgggccggca cggaggctac
     5041 cagaacggtg gccgcgatgt tctccccgta atccaggagg ggacccgcca ggaccagggc
     5101 ccgcagggcc agggcgaagg cgccggggct cgaatccgcg cgccgcaggg cgaaggccgc
     5161 ggccgaggcg aagagtgccc cgtagaccag gggccaagcc aggtccatgg tccagcggtc
     5221 cgagatgtag gaaaagcgcc ccggcgcgtc gaaggccgcc gccttgtcca gggcctgccg
     5281 gggaccgtaa aagaagctcg tgtcgaaacc tccgcccgcg ggggtgtagc ccgccgcctt
     5341 ccgggcgtag ggtaccagca ccaggggcac aaaggccgcg aacagggccg ccgcggccgc
     5401 caggaaccag gcggaaccca ggatcctcca gaatctctcc gacataggac ctccccgccc
     5461 ctccgggccg gatgtaacca agccgccgta gtatcattgt agtacgtcag gggacgggcc
     5521 ggaagggaca tagcacccgc ccgcccccgc cgtccggccc cgcgccggcg gctaattcga
     5581 tccttcgaga ggccccatgt ccagatcccg gcgaccgatg cgatacctgc ccgccctgcc
     5641 ggcggccgcg atccttctcg cggccctgtc ctcctgcgcg gcgggcctct ccctggccgt
     5701 gaaccccgac gggtcgggga ccctggccgt ggacgcccgg atccccgacg ctgcggcggc
     5761 ccgcctgcgg tcctacgcgg ccgccgggac cccgggttcc gctccgacgg cgcccctctt
     5821 cgacaccgcc gcggtccgcc gagagaccgc ccgacgcggg ctggccgcgg tatccgtgga
     5881 aacccccacc ccgaaccgtt tccggggcag cttctccttc cggagcctgg cggaactggc
     5941 cgcggacccc gaactggcgg agtcgggcat tctgcgggtg ggatcgaagg acggcgtcac
     6001 cgccctgtcc ttccggcttt cccgggacaa cgcccgatcc ctgcccctgc tcttccccgg
     6061 gctggatccc tacgtgttgg aggccctctc gcctccggcc ctggatccct atcccgtgtc
     6121 ggcctccgag taccgggaga tgctccaggc cctcctgggc acggcggccc tgggggaact
     6181 ggattccgcg gagatccgta tccaggtccg ggcccccgac cccgtgctcc gccacgcggg
     6241 aggcgcggtc tcggaccgca ccttcaccgc ggtcctgcac ctgatggaca tcctggtcct
     6301 ggaaaaaccg atggagttct ccgtatcctg gaggtagccc gggaggccgc ttcccgggcc
     6361 ggcccgatca agccttgaac cggacgacgc cggtcgtccc ctcgccgccc cggatcgaga
     6421 aactgccgtt gagctgctcg gccagcatcc ggaccagctg gaggccgaag cccgtcgagg
     6481 attccggatc gaacccttcg ggaaggcctc ggccgtcgtc cttcacttcc aggacgatct
     6541 cgttcccctc ccgggccacc cggatcccca ggctaccccg atcgcgcccc gggaaggcgt
     6601 gtttcatgga attcgtgatc aactcgttga cgatggtccc cagggggaag acggtccggg
     6661 tcccgagttc gaattcctgt atgtccggtt cgatccggat cctcccggta tccgggaaga
     6721 ggtccaggat ggcggccagc aatccttcca gatactcccg gacggagacc tcccggtagt
     6781 ccccgctgac caggagcttg tcgtagacca cccgcatgct ctccacccgg gccgcggcgt
     6841 ccaggatggc ggtgcgggaa gcgtcgtcgg gtacggaatc aagctggagc cggaggactc
     6901 ccccgatgga ggcgaggttg ttcttgatcc ggtggtggac ctcccggagc agggtctcct
     6961 tttcccgtag ctgccgctcc agggcgtcct cggcccggcg ccgccggacg gtcttccaga
     7021 cggcgtccat cataagggtc agctggcgga catccgactc gtcgtactcc gcctccttgt
     7081 tggccacccc cgccacggcg atgatcctgc cttcatcgaa gacgggcacg gtcaggaagc
     7141 ggcgcagcgg ggcatgcccc tcgggaagac ccttcttgag ggggctcggc gcggcatact
     7201 cgttgaccaa gatcggcttc ctctgccgga cggcctcccc ccagattccg gtcttgtcga
     7261 gttcgtacac ggtctgcttt tccaggaccg tgcattgatg cagaacctcc ttggaccagg
     7321 tgttcagggt gaacagcttc cgatcctcgt cgtacaggta gatgtacccg atccggcttt
     7381 cggtcaggag gatggcctcg tccagtgcgt agtccaggag gtcctggatg tcccgggcgg
     7441 ggtactggtt gatcctcagg aggctggcca gtcgggcctc gctccgacgg atctcgcggt
     7501 ccgcccgcac ccgttccgtc ctgtcccggg ctatactgag catcgcgaag cgtccgcccc
     7561 agtccgccgt ctggaccgtc acctccaggg ggaggagcgt gccgtctttc cgccggtggg
     7621 tcacttcgaa cgtggcttcc ccggcgtccc ggattttccg gatacgttcc tcgatgagca
     7681 cctcgctctc cggcacgtcc aggtcgtgga ggttgagggc catgaactcg tcctcggagt
     7741 atccgtgaag ggcgaaggtc ctgcggttgg catagaggaa ggcgccggat tcgtcgtgga
     7801 tcgtgatgga gtccggggcg gcgtccagga gccggcccag cagggtgatg cgctcctcct
     7861 tggcccgggc ctgcaccttc gcctcgaaga gccggaaggc catcttgatg gacgccacaa
     7921 ggacgttctc gtccgagttc ttgaccacgt agccgtagga ggtgatcccc tcggtccgtt
     7981 ccaccacctc ccgttcggtg tgcgaggaaa ggaagatcag gggaaggtcc cgggccgcca
     8041 ggatccgctt cgctgcttcc gtgccgtcga tgcccgctcc caggttgatg tccatgagga
     8101 ccaggtcgac gtcctgctcg gaggcggccg cgaccgcccc ttccccgtcc gctgcggtcc
     8161 ggacccggaa accgtacctc tccagggtcc tccgctccgc cagagctatg agggcctcgt
     8221 cctcgacgag gagcagggtc ttcaaagggc cggcgtcggg ggtcatatcg aattaagtat
     8281 ttcgcatcct cgccgaatgt caaacgaggt acatggcgag acgggaacgg gtccagaatg
     8341 aaacctaggg agtcgaaccg attccggttt ccttccgcag gaatgcggac aaagcctcga
     8401 gcatcccgac tccggaaggg ctcgcatact ctggagcgtc gccggctcca gcggaacgct
     8461 gcagctcggc tatagcctcc aggcaaccct cgatgggaac ctcgacgagg gcgtagatct
     8521 tcctcgtgga gacgaagtcc gtatcctttg gagccgcgtt cggggctcgc cggaggatct
     8581 cgatccagta ggtagaaagg cgtgacccgg aagagagact gcgggcgaga tcatccgcca
     8641 ggatcgccgc ggtcgcacga tccgaatccg acggattctc cagagtcgaa acaacaaagg
     8701 gcagtatggt atcccgaagt acccgttcaa ctgtcaactt cttcgtcgaa gccatgctgg
     8761 ccctcgagta ccgtagcgcc aggaaaccgg aatactgagg gagaccggtg agcagaaggc
     8821 gctcgtccgt cacccattcg gggatgggct tctcggggtc ggatcgcccc ttaaggaaga
     8881 cggtgtgccc cagggtccgg cctttgaggt atttgtccaa ggatagcacc gcgaagcggc
     8941 cactcgggta ggtcgccagc tcatagaaac taccctgcca ggaaaatctg tatccgtccg
     9001 gtgaaaatgc gggcagactc atgcgttcca ctttatgccc ggccgttgag atcaccgtca
     9061 gttccccgtc tgccgattgt cggaccggga agcgaagctc ctcggccgtg cccatcgcgg
     9121 agagggacag cgcggaaacc aggaacatca gggcgggaag ggatttgcgg gcgatgggca
     9181 tcgtatcctc cacggccatc ctatccaatt gtcagtaccc aatcaagcga gcccggctac
     9241 cgaagcaccc ccgactcctg gacagcggcc gcaccctcgg ctaccattcc cggcatgaaa
     9301 ggcgtcatcg tcgcggcggg ctacggtacc cgcttcctgc cggtcaccaa gacggtcccc
     9361 aaggagatgc ttcccctcct cacgaagccc agcatcgcct tcgtggtgga ggagttcgcg
     9421 gcctcgggga tccgggacat cctgattctg accagccggc gcaagaaggc cctggaggac
     9481 ttcttcgacc gggatccgga gctggaatcc gtgttcaagg ccgaaggcgc ggcgggcaag
     9541 ctcgagcgga tcgccccgcc cgcggtcaac gtatccttcg tccgccagcc cgagatgcgg
     9601 ggcacgggcc acgccctgct ccaggcagca ccctggctgg gcggggatcc ttgcgtggtg
     9661 gcctatcccg acgacctcca tttcggctcg ccccccctgg cccgccagct catggacgtg
     9721 tacgagcgca cggggtgctc cgtgctctcg ggactccacg agcccgggga cgtgtcccgc
     9781 tacggggtca tcgaccccgc cccggacggc gtccatgtcc ggggcttcgt ggaaaagccc
     9841 gccccgggga ccgagcccag ccgggagatc tccctgggcc ggtacctgta cactccggaa
     9901 ttcttcgaga tccttgcgga gggctgggcc aggcatacgg gaggggaata ctaccacgcc
     9961 tacgccctgg acagactcat cgccgcggga aaggtcgcct gggccccggt cacgggcacc
    10021 cgcctggaca ccggggatcc cgcgggtttc ctggacgccc tgctgcgctt cgcctgggag
    10081 gatccgggat accgaagcgt cctcgaatcc ttcatccggg aaaaaaccgg gaagccggcc
    10141 ctatgacgcg gggccggagg gaggaaccat gaaggtcgtg ctggtccgaa tacaggtcaa
    10201 gccgggcgcc gaggaggcct tcctggcggc caccctggag aacgcccggg cttccaaccg
    10261 tgagcccggg gtggcccggt tcgacgtgct ccgggacgcg ggggaccctt cccgcttcgt
    10321 ccttgtggag gcctaccggg acgaggaagc ccccgcccgg cacaaggaga ccgcccacta
    10381 cctccggtgg aaggagatcg ccgaacccct catggccggg gaacggaccc gggagttcta
    10441 cgaaggcgcc tacgtcccgg agcgggacct ccggtgacct ggggattcgg aggacccggg
    10501 gagatcctct tcggggaggg cgccgtacgg gagctcggca agcgggccgc cgccctgggg
    10561 acccgggcct tcgtggtgac cggccgggat cccggccggg ccgccgcggc cctggattcc
    10621 ctgcaggccg ccggtcttga ggtcgcggta ttccccgccc ccggggaacc caccttcacc
    10681 gtcctgcgat ccgccctggc ggaggcccgg aagtacgccg gtccgcccgc cgagggcgcg
    10741 cacgatgcaa gcgatccggc ggctccgcct cccgggctcg tcgtggtcgg cttcggaggg
    10801 ggcagcgccc tggacctggc caagggtctg ggcatcctgc tttcgaatcc cggggacccc
    10861 ctggactacg cggaggtgat cggcgcaggc cggcccttca cggccccctc ggtccccgtg
    10921 atcgccgtgc ccaccaccgc gggcaccgga agcgaggcga ccaagaacgc cgtcttcgcg
    10981 gaaccctccc ggggcgtgaa ggtatccctc cggtcaccgc acatgtatcc gcggctggcc
    11041 ctggtggatc ccgggctctg cctggggctc ccatccgaag tcacggcctt caccgggctg
    11101 gacgccctga cccaggtcct cgagcccttc gtctgcctca aggcgaaccc cgcgaccgac
    11161 gccctctgtg cggccgccct ggcggaaacg gcgccggcct tgcgccgggc cttccgggac
    11221 ggcggcgacc tggccgcgcg ctcgatccta tcccgggtct ccctgttcag cggcctggcc
    11281 ctggggaacg cgggtctcgg ggccatgcac ggcttggcgg cccccctggg cggacgcttc
    11341 ccggtgcccc acggggccgc ctgcgcggcc ctcctggcgc cggccgcggc ggtcaacatc
    11401 cgggccctcc ggaaccgcga ctccggaggc cccgccctgg accgctacgc ccgggccgcc
    11461 cgcctggtga ccgggaacgc cgcggccggg ccggaagacc tcccggccgc cctggcggac
    11521 ctggccgcag acctgggcat ccccgggctt tccgtctggg gcgtccggga ggccgacctg
    11581 cccggaatcg cggcggacgc ggggacttcc agctccatga aggcgaatcc cgtcgcactc
    11641 tcggctccgg agaccgaggg catcctccgg gcggcgctgt aacgggaacc gagagtctcg
    11701 aagcgccggc ccgccccggg gccccgtggg cttccaacct cgaaacaccc tagacggaaa
    11761 agcccgaagg ggctattatt tggcataagc ccattaaaga ggatatgcca tgaacgagga
    11821 gaaaggcgcg ggaaacgcga atccccgtat agcccgggtc gtcggggttg ccagcggcaa
    11881 ggggggcgtg ggcaagtcca ccatcgccgt cctcctggcc caggccctgg cggccaaggg
    11941 ttcccgggtg gggatcctgg acgcggacat cacgggcccc agcgtgcccc gccttctggg
    12001 ggtggattcc ttccgcgccg aggcggacga cggcaaactc gtgccgatca agtccgacga
    12061 cgggatcgag gtgatgtcca tcaacttcct gatcgaggag gagacccggc cggtcatctg
    12121 gcgcggcccc cttttgtccc aggcggtcaa gcagttctgg accgacacgg cctggggaga
    12181 cctggactgg ctgatcgtgg acctgccccc gggcaccagc gatgtcatgc tgacggccct
    12241 ccagagcctc cccttcgacg gccttctgtt cgcggccacg ccccaggact tcgtgtccat
    12301 gatcgtggcc aaggccgtcc acatggccgg tcagacggac acccggaccc tcggcctggt
    12361 gttgaacatg gtatccatgg tctgcccgca ctgcgggaag gagatacccc tgttcaccgg
    12421 cggaggtacc gacgacccgg acctgggcat acccgtcctg gcccggatgc cctggcgggc
    12481 cgacctggcc caaaagcgcc acctcctctg ggacggcctg tccgaagagg tccgcaggac
    12541 cgcttcggac ctggccgagg gcgtcgcagg aaccctggcc cgggcggagg tacggtagca
    12601 tgccccgccc cgtctcggag cgccgcgtcc ggggctggcc ccggtcccgg gccttcaagc
    12661 ccgcggggat acccctgcgg gaactcgaga ccgtgatcct gaccctggac gagctcgagt
    12721 cggtccgcct ggcggacctt gaggggctct accaggaagc cgcggcggcc cgtatgggcg
    12781 tatcccggca gacattcggg cgcatcgtag aaaccgcgcg ccgcaagatc gcggacgccc
    12841 tggtgaacgg aaaggcgctg gaaatagacg gaggaatcgt acagatggcc gagacggaca
    12901 tgaacgagta caggatcgcc gtccccaccc gggacgggat ggtggacccc catttcgggt
    12961 cctgccgggg cttcaccctc ttcgacgtca aggacgggac cgtggtcaag gaggagtact
    13021 tccccgctcc cgagggtgcg ggctgcaaga gcgccgccgc ctcgacctta gccaaggccg
    13081 gggtccgggt gatgctggcc ggcggcatgg gcgaaggcgc cgtgcgggtg atggcatcct
    13141 tcgggatcac ccccgtgcgg ggggtcacag gtcctgtgcg ccccgtcgcg gaggcctggg
    13201 ccgccgggaa gctctccgac tccggggagg tctgctccca cgaccacgaa ggacacgact
    13261 gctcgcacca ctcctagggg atcccgcggc acggcggaag cccgaagcct tcgaaccgcc
    13321 ccggatcccg gggcggtttt ctttcacgcc cccggccgcg ctatactgga atccggaggc
    13381 cgcctatgaa aagttccccc tccccgacta tcaagccgaa gcgggcacgc cggccggggc
    13441 gccccgccgg cgcccggatt ttcgcggcgg tcttggcggc ggccctgctc gcgtcctgcg
    13501 ccgggatgag cggcagcgcc tacgagcgct ccgccgagtc cgattcctcc gcggcggccg
    13561 tcgcggccca gcggaagaaa gactcccctc ccccgtccca accccggacc cctcccgccc
    13621 cggagtccgg tacctcgggg ttcctgttct tcggaccccc gcctccaccg cccccccggg
    13681 gatggatcac ggtccggggc ctgccctccg gcgctaccct ctacgtcgat gatcgtccct
    13741 actccggagg ctccgcggag gtcgaggcgg gcgaccgttt cgtcgaggtc cgggccttcg
    13801 gctacgaacc ctggtcggcc cgggtcacgg tccggatcgg agaatccgtc gaactcgagg
    13861 ccgggatctt ccccgcggtc ttccggctca cggaactgga agccgaaccg gacaccttcg
    13921 accccggagc acccggcgcc ctggggcgga cccggctgga atggacggtc acagctcccg
    13981 gccggggaga cctggagatc cgggataccc aaggccggac ggtgttcgcg gcccgggaca
    14041 tcgagctcga ttccgcgtac ggggatctcg cctgggacgg ccgggacggc tcgggtcgct
    14101 cggccgcgcc cggggtctac tacgcccgcc tttccgcggc aggccgggac ggttcggcct
    14161 cctccctgga gacctccgtg gaggtaagcc ccgaggccgc cccccagcgc ttctccggcc
    14221 tccatggggg attctcgggc gccctcttcg ccccggatgc ccgagtcctg ccggaaggct
    14281 ccttccaggc ggcggcggga acctacctac tctcgacgcc ccaggacgcc gattccgcgg
    14341 cccggatgcc ggccttcgcg gggatccggg cgggagggct cttcggaggg agatcggagc
    14401 tcgtcgcgtc ggggatgctg gtaccctact tcgggttttc cggggcggat ccctcctggg
    14461 gctccctcat ggtgtcggtc aagacgacct tggcggcggg gcctcacggg gccgccgcct
    14521 tcctggcctc cgcctcggtc gcgagcttcc tggacgaagc ctcctcgggc ttcccgcccg
    14581 cctgggacgg cccggcccgc taccccggcc tcggggccgg cctcgtcctg gaggcctcca
    14641 cccggacctc ccgggtcttc gccagcctcc acgcccacgc ctcgacctac taccccgact
    14701 gggacggcct cgaatgggac actccggggc tcttcgcctg ggcctacggc cgggcggggg
    14761 cggaggccct gatttcgggg gtgttcggag gggacctggc cctggccctg tcggcggcga
    14821 cccgtaccga gcccttcggc ggagcgaggg gattccggcc gcccctgtcc gtcggcgccg
    14881 aggcgcactg gtacgccccg gagtccggcc tggtcctgtc cgcctacgcc gccggggaat
    14941 gggctgcctt caaaagctgg tactacgcgg ggggcttcgg gatgggcttc gcgttctaga
    15001 aggacatttt cccggaatca accacggagt gcgcgccaag ccggcgatgc cggcttggcg
    15061 gcgccgaagc aagcttcggc gaattgccca caggtcaacc ggcttccatc gagccggtct
    15121 ccaggaattc ggcgaagccc ggcagggctt cgctgccgta ggtccggcgc agccggacct
    15181 acgttacata ccgtacggaa agagcgcgcc ctgcttgtcc cggaggcgct cggcgatggt
    15241 ggaggcgatg gcccggaagt ccgcggactc ctccacgaag tccaggcggt ccccgggcac
    15301 caccaggaca ggagcccggt cgaagccccc gatccactcc tcgtagagcc tgttcaggcc
    15361 cgcaaggtat tcgtcgggta tcgacgcctc gaagtcccgg ccccgcttgg cgatccgctt
    15421 cttgagggtc gggacggagg cccggaggta aatgaccagg tccggcggcg ggaggaggtc
    15481 ggccacggtg gtgtagagct cccggtacgt ggaccagtcc cgcccgctca gggccccctg
    15541 ctcgcgcagg ttgcgggcga agacctcggc gtcctcgtag agggagcggt cctggaccac
    15601 cgagcaggga tcgtccatga gggcgcggtg ggtcttgacc cgatgggtca ggaagaagag
    15661 ctggctctgg agggcccagc ggcccatgtc cgcgtagaaa tccgtgaggt agggattctc
    15721 cgccacgggc tcgtagtagg gcatgaaccc caggcgcttg gccagaaggc cgaccagggt
    15781 ggacttcccc gcgccgatgt tccccgccag gaccaggtac ttcttcatcc ccgctttttc
    15841 ttcacgtcct ccgccttgcg ggagatccgg ttccacaccg ccgagagccc catggccttc
    15901 ttggccgccg taagggtggc ccgggcctcc tcccgcattc ggagagtgcc gtcgaacacg
    15961 acctcgtccg ccaggcccga ctgggcttcg tacttcgcgc ggcgctcccg cagggggtcc
    16021 aggaaggcgt tcagggcctt ggccagcttc tccttcacct ccacgtcccc gaccttgccg
    16081 gcccggtacc gggccttgag gtcctcgacc tcggccttgt cgggattgaa ggcgtcgtgg
    16141 tactggaaga ccgggttgcc ctccaccgtt cccgggacgt ccgcgctgac ccgcttgggg
    16201 tccgtgtaca tggacatgac gcgcttgtgc acggtcttct cgtcgtcgga caggaagatg
    16261 gcgttgttga ggctcttgga catcttcgcg ttcccgtcca gtccgaccag ggagccgaac
    16321 tccccctcca tgacgtcggg caccgggaag acctcgccgt agaggttgtt gaagcgccgg
    16381 gcgatctccc gggtcagctc cacgtgggcc acgttgtcct tgcccaccgg caccaggtgc
    16441 gcccggggca ggaggatgtc cgcggcctgg aggaccgggt aacctaaaag gccgaagggc
    16501 atttcgtcca ggccggcacc ccgggccatt tccttgaggc tggggatccg ggacaaacgt
    16561 ggcacggtga ccaggttctg gaagaagagg gacagttcca ccacctcggg caccgcggat
    16621 tggaggtaga ttacgctccg ggccgggtcg atgccgcagg ccaggtagtc caggaccgcg
    16681 gagcggacgt tgtccgccag gccctcgatg ttttccttct cgggcttggt ggtcagggtg
    16741 tggaggtccg cgatgatgaa gaagcagtcg tactcgtcct ggagccggac ccggttggcc
    16801 agggagccga cgtagtgtcc gagatggagt ttaccggtgg ggcggtcgcc cgtgaggatg
    16861 cgcttgtgtt ccatggcgag ggatcatagc ccgaaggagc gaggacaggc aaggcggacg
    16921 gaccccctca ggccgaaccg cccaggcggt cgtcgtcgaa tacgacgacg cactccaggc
    16981 ccggggagtt ccggatctgg agggtcccgc ccagctgacc ctccgccagc atcttcacga
    17041 gccggagtcc cagccagggc cgcgggatgt cccggaggtc cacctcgaga cccgggccgt
    17101 cgtccgccac ctggagctcg acccgcccgt ccccgaggcg ccgggccgtc accgagaccc
    17161 gtcccggcct gcccggaacc ctgccgtgga ccatcgcgtt gtccacaagc tccgccagga
    17221 ggatcccgca ggggaccgcg gcctcgatgt tcaaatcgac gccgtccgcg tccaggaaga
    17281 gttccgccgc cggcccgccc ggaatccggg aggaagtctc caggaaccgg gagaggaaat
    17341 ccagcaggga gatacgggag acgtcccgcg ccgtgtagag gcttcggtgc acggaggcga
    17401 tggcggccac ccgtctgcgc atccgggaga ccaggtcgtc gacccgtcga tcctcgtccc
    17461 gttccgcctc gagcatgagc agggacagca ccacctggag ggtgttgttg atcctgtgga
    17521 acagctcctg gatgaggagg tcccgttccg cgatggcctt ccggagcccc tcctccgccc
    17581 gcttttcgtc cgtgacgtcc cggatgatcc cgaagagccg cttcttcgcc ccgtcccaaa
    17641 gggcccggga ccggaggtcc aggatccggc cgtcgctccg cctgcaaatc ttgaattcca
    17701 cgtcgtaggg aaggcctcgg aggatgtggt cgtcccgggc cttgttcagg agtggacgat
    17761 aatcggggag gggtatcgtt tcgatgtccc cgacggtcag gttctcccgg gggacgccgt
    17821 aaatcctcgc cgcgccctcg gagcccgtca cgaccttggt cgcgtagtcg aactcccagc
    17881 tcccgatctt ggccatctcc tcggcccgtt ccagcaggaa ggcgctcttg cgctccacgg
    17941 cggcggcttc cgccgcggcc gtgatgtccc ggcagatcat caccagggca ggtctgccgc
    18001 cggccaccag aggggaggcg gaaagctggt acctccgctc ctcccccgac ttggaacgga
    18061 gccgggcctc gaagccccga acgggttcac cgcgacccag gcgtcctgcg aaccgctctc
    18121 gttccccggg atccgcccag atccccaggg agaggctgga ctttcccagt acttcctccc
    18181 gggtgtaccc ggtcagctgg acgaaagccg ggttgacctc gagaaaggtc ccgtcctcca
    18241 gggtggacac ggtgatgatg tccggcgtgg actcgaatat agcctggagg agggattccc
    18301 ggtcccggga ttctgcatcc ttcatgcgac aattctctcc atcgggcgcc cgaaatcaag
    18361 ccgtccgggg ctcgcccggt ccggccgggt gccgtgctcc ggtccgggaa aatagacgaa
    18421 gcccttcggg cttcgttgcc gtgacgaact acgggccgcc catggcccgg gaaaatagac
    18481 gaagcccttc gggcttcgtt gccttgacga actacgggcc ggccacggcc cgtagttcgt
    18541 caagcctaat cctccaggaa caggcccccg ctccccaggg cctccgcctt ggagccgaag
    18601 aactgtccca ggggcgagcc ggcggatgcc cacttgaaca ggagcaggga ccggcgcacg
    18661 taggccagct tgtcggccac ccccggggct tccgcccggt aggccccgaa aaaggcggcc
    18721 gtggtccggc cgtcgggcag ggtcccggcc ccgacgcccc ggtcgtgcag ggcggagagc
    18781 tcctgcaggc ggaagagggg ccgctccagc ttgaacaagg ccgcaaggca gtcgaagagg
    18841 acgggagcca cagccttctt ctcgggtatc cagtccgtga acttgggttc gcctccggcc
    18901 tcccgggcca ccgcccaggc atccaagacg gacccctggg ccttcagctt ggcataggcg
    18961 gcctcgaagg cgggcagggc ttccgtgtag cggccggcca agccggctat gacccggggg
    19021 gcccgggctt ccagggattt ctggccgtca tccacccagg ccacgcggaa gtccgccagg
    19081 gtttccaggg ccaccaggtc ctcccggatg gattccccgg ccttctggga gacgatccgg
    19141 ccgggctggg cgtagccccg ctcccccgaa cctccggtca cctcgacgcg gccttccgcg
    19201 caggtcacca gcagggcccc gtcggggatg gtgttgacga tgaaggtggt tccccggacc
    19261 ccgaggacgg cgctgtccgt gccgaccttg aattggccgt ccgcgagctt ctccaccttc
    19321 acggccaccg agcccgccag gagctggagc accgtcctgc gggcctcgtc gtccgagagg
    19381 gcggtctcgt agtagaaggc cgtgttctcc gccaggcgga tctccgcgcc tccggggctg
    19441 ccggggtcga gttccagggt ggcccggctc ttcgggcccg tctggaggac gtcgaagttc
    19501 tccaccacct ggccgatgtc ggcggggcgg ggagcggagc cgccccggtg cagggtgacc
    19561 gtcccctcgg cgtatacgag ttccccgatc ctgcgggggg ccttccgggc ggtttccggg
    19621 gcgggggcgc ccggggaagt ctgtccgggg acggccgggg cggcggcgcc cggggcggcg
    19681 gccccgggcg cggtagccgg cgagccgggg gttccggagg actgcccggc ctcggccgac
    19741 ccggtcccgg ccgcggcccc ggaggggccg ggggagggtg gcgtttcggg ggcctgggcg
    19801 ccccgggcgg cctcgggaac cggggaggac gaggggccgg ggcgggcgcc gcatccggaa
    19861 aggaacagga cagccccggc gagggcgagg atggaacatt tccgcaggac cttcatggcg
    19921 tcctccgctg cgcaagctgg accgggcgta ccgctggaaa cctcggtccg ttatagtata
    19981 taacgctttc aacgccgtag tcggccgatt cccgcaaaaa cccgacgtcc ccgaacgcgg
    20041 gtccgggata atcccccgca ggaggccgtt cccatgaaga aatacctcgt cccggcgaac
    20101 gccgttgcgt acgccgttgc gatcaccgcc gcgatcgccg tctcgatcgc cctggccggt
    20161 tgcgtcagcg cccccccgga gaaagccccg gagccccggc gcaaggaacg ggtggagact
    20221 gtccgggtac ccgtcctgct caaggagagc tcctacttcg ccgacggggt tttggaccgg
    20281 acggcaacct acacgtacga caccaccctg aaattcctcc tggagaaacg ggtccaggaa
    20341 ccctcccgta cggaacccac ggagcggatc gtgtacgagt acacggggac cgctccggcc
    20401 tcgatctccg tctacgactt tgaaggaaaa ctcaggacgc gtacggactt caaggtcgac
    20461 gccgcgggtc gggttctcca ggagacggtc tccgatcccc gggggactcc ccagtcccag
    20521 gtccgatacg aatacgatcc ggccgggctc cgcacgagcc gccgggtgta cgacggagcc
    20581 ggcgccctcc tggcgatttc cgaatactcc tacaccgcgg gccgcctggc cgtcgtgatc
    20641 ctgaaggatt ccgcggaacg ccccgcgggc cgggtggaac acgaatacga ccccgagggc
    20701 aggctcgtcg tccgggtcgg cttcgacccc tcgggggccg tggcccagcg ggagcgcttc
    20761 gcctatgcgg gcggcgccct gaccgaggaa cggctggagc gcggggacgg ccgggtggag
    20821 cgccgtacgg tccacgaggc cggccccggg ggcgcccccg caaaatccac ggtgtacgac
    20881 ggatccggcc ggatacggga cgtccgcgtc tacgagtacg ggttccgctc cgaggagcgg
    20941 accgtcgtct actacgaatg atccggagga tgggaatgaa gcgaagcccc aagatcctgg
    21001 ccgtcctggc cctggcctgc ctgacggccc tggcctcggc cgcggacgag atgaccttca
    21061 tctaccggcg catctaccag caggccgaga ccctccggca gaaggaagcc gccgccctgg
    21121 cggtcgtctc ccgggacgat cccgggaccg cgtccgtcct ggaatacatc ctgggggacc
    21181 tgctcaggtc cgggcccgag gtggccaagg gttcccagga ccggcaggtc tggaacaacc
    21241 tggtgcggac caccgtcggg gccctcggca cggcccggta cgtcgaggcc gcccccctgg
    21301 cctgggacgc cttcaaccag agcgaggatc ccctggtgcg ggcggaggcc ctgatggccc
    21361 tggggaacat gcgggccctg gagtacgccg agcggatcgc cctggtcctg cggaacctca
    21421 acttcgagcc cgtccaggac cgggaggcgg gggagaagac ggccttcgga gccatcctgg
    21481 ctttggagaa gctccgggac cccgtcggat tcgccccggt cttcttcgcc tccgagggct
    21541 ggtacacccg gcgcgtccgg gaccaggccc tgcgcagcct gcccctcctg gcggaagacc
    21601 ccacggagcc ggtgcgcagg ctcatcctcg aggaatccct ggaccgcaag ctcagggcct
    21661 tcgacctcca cgcggcatcc aaggctccgg aggagaggaa gatcgagata gcccgcctgg
    21721 ccctgcagag ggggatcgag atcaaggccc gggaccgggc ggagggtacc aagctccggg
    21781 acctccgggt ccggggcctg cgctccctgg tggccctgaa ggcccgggga ggagcctcga
    21841 cggactggat ccgggaagcc ttccggctgg gggagacgga cgagcgcctc ctggccctcc
    21901 aggccctggg caccgacgcc tccgacccct ccgcccgggc cctggcggag atcctcctgg
    21961 acctggacgc ccagcagcgg gcggggatcg ccgacgaggt ccgcaaccgc atcgccgcgg
    22021 cggccatcca gtacgccggg gccacccgga accgcctggt ccggccggcc ctcacggcgg
    22081 tctccctgaa cgacacgtgg tccaattccc tgatccgggc ggcccaggac gccctgaagg
    22141 agctaccatg aacgacgacg cgaagacctt cgccgcccgc cgcgggcgga tagccgaatg
    22201 gctcaaggag cggggcatag ccgccgcggt cttcgaggac cgggagggcc ggagggaccc
    22261 cacggtgcgc tacctctccg ggcagcccgg ggactccctg ctcctggtca ccgccgcggg
    22321 ccggggggtc ctggtctcct gggacgtgaa catggcgcgg aagctgtccc gggtcgacga
    22381 gatcctggcc tacggggact tcgagcggaa gcccgtccgg gccgtcctgg gcgtcctggc
    22441 ccgggaaggg atccggcccg ggtcccaggt ggagctcccc tccaccctgg cctatccgga
    22501 gtacatccgc tacgccgagg agggggccga ctttgacttc ctctgccggg aggacggggc
    22561 ggacgagttc gcccggggac tccgggcggt gaaggacgag tccgagatcg ccgtgtaccg
    22621 ccgcgcctcg gccgtcacgg acgagctcgt cgaggccctg gaggccgggg tccggaaggg
    22681 gacgatctcc acggagtccg acgcggccct cttcctggag cgcgagagcc gggcccgggg
    22741 ctgcgagggc atgggcttcg agaccctggc cgcgggaccg gcccggtcct tcgggatcca
    22801 cgccttcccg acctacactt ccggggcctt cgggaccgcc gggctttcca tcctggactt
    22861 cggggtctgc ctggagggct acaccacgga cgtcaccctg accttcgtca gcggacgacc
    22921 ctcggccctg caggagcgga tgatcgccct ggtggtggaa gcctacaagg aagccgcggg
    22981 gatgctgcgc cccggggtcc ccaccctcga ggtggcccgc cgggtggacg agatcttcgc
    23041 cgcggggggc ttcaagatgc cccacgccct gggacacggg atcggcctgg aggcccacga
    23101 ggccccggcc atgcggaacc gggacgacaa cccctgggtc ctggcccccg gccacatcgt
    23161 caccctggag cccggactct acgatccgga ggcgggaggg gtgcgcctgg agaacgactt
    23221 cctggtcctc cccggcggcg cggaagccct gacccgttcc cgcatcgtgc ggctgtgaat
    23281 ccccgcctcc gggagggaca tccccgcatc gtccaggcgg ggcccatcct ggcgatccac
    23341 aagaccctct tcgtggaccg ccccttgctg tccctggcgg gcgcggcggc ggtctacgtc
    23401 ttcatcgtcc tgggcttcgg ccgaagcctc gagatctcca gcaactactt cgtcctcctg
    23461 ccggtcctgg cggccgccct gggctacgga ctggccgggg gcctggtcgc gggggccctg
    23521 ggcctcccgg ccaacctcct gctcttcaac ctgctgggcc ggcccgagtt ctcccccgcc
    23581 agcaagccca tcgcggagct ctccggcctc ctggtggggg gcgcctcggg ctacctggcc
    23641 gagtacttcc gccagctcca gtccgagatc gcccgcagga tccggacgga ggaatccctg
    23701 cggagggccc tggaggacaa gcaggtgctc ctcctggaat tgaaccaccg ggtcaagaac
    23761 aacctcaacg ttatcaagag tctgatccag ctgcagagga accggtccga agccccggag
    23821 ttccgggagg cctcggacag gctcctggcc cgggtcttcg ccatagcccg ggcccacgac
    23881 cgaatgttcg gggaggacgg tcccccggtg ctcgacccgg tcgagtacct ccaggacatc
    23941 ctggcggtct acgacgccga ggcggggatc gggccccgga tcgaggcgaa cctccgcccc
    24001 gacggcctgc ggcttcccgc ggagaccgcg gtgcccctgg ggctcatcct gaacgaggcg
    24061 gtggctaacg cctggaagta cggatcctcg cgggaagaag ggaccatccg ggtctccctg
    24121 gcccggaccg gggccggctg cgtcctggag gttcgggact cgggaccggg cttcgacccg
    24181 gacgccgtcg gccgcccgga ccggcctcag ggcctgggag tcaagatcat acgcagcctg
    24241 gcggcccggc tgggagggcg ggtgtcctgg tcccgggagg ggggaccccg cttccggctg
    24301 gaattccccg cggccgccga ccccggcaaa accggatccg tcgttgccga tcccgggccc
    24361 ggaaggctat aatccccccg gaccaatcga tgaagaccac atcagccgcg tacatcatgg
    24421 agaacgacca ggagagccgc cgcctggaat tgaagaccga cgtccgggtc gtgcgggacc
    24481 aggcccggag ggccggcctc gaaccgggca tgcgggtcct ggacgcggtc tgcgggcccg
    24541 ggatcaccac cgcggccctg gcggaggagg ccggggaggg agggtccgcg gtcggcatcg
    24601 acttctccga ggaccgcatc gcccgcgcgc gggaggccca cgggggggct cggaccgagt
    24661 tcctggtccg ggacatcctg gaacccctgg aaggcctggg aaccttcgac ttcatctgga
    24721 tgcgcttcgc cctggagtac tatcgggccg agagcttcga catcgtccgg aatctggcct
    24781 cggtcctccg ggagggcggg atcctctgcc tcgtggacct ggactacaac tgcctttccc
    24841 accacggcat gtccgggcgc ctggaaaagg ccctgcgcca cgtggtggac accctggagg
    24901 cccgggcgaa cttcgatccc tacgcgggcc gcaagctcta ttcccacctg tacaggctcg
    24961 ggttccggga catacaggtc gaggcgggag cccaccacct gatctacggc cccctgcggg
    25021 aagtggacga gtacaactgg acccggaaga tggatgtcct cggagaccgg atatccggag
    25081 acctgccggg ctattcgggg ctggatgaat tccggaagga tttcctggcc ttcttccggg
    25141 atcccggacg gttcacctac acccccgtgc tgtccgcctg gggccggaag cccccggccg
    25201 gggcctagca ggttgtcgat acacccgatg cattccacgc cccgctagag ggcggcagcc
    25261 tccgggcccg ggacgaccgg agcgccccgg gccgcagcca cgaggccgga tccggggggt
    25321 accggggcag ccggacgtgc gaccgctccg cccgggaccg cccctcgcgg agccggtccg
    25381 gccccgccgc ccgggaccgc cggggcgccg gggaccgcgg tccggccctt cacgatggcc
    25441 cggagcaggg cttcgataac ggcctcgtcc aggtgccggc ccccctcgcg tttcagggtc
    25501 tccacggctt cctcgaaacg gaggggctcg tggtagtgcc gcttggccgt gatggcctcg
    25561 aagaagtccg cgaccgccag gatccgggcg cccaggggga tctcctcccc ccggagcccc
    25621 cggggatagc cggtcccgtc caggcgctcg tggtgggacc cggcgatgaa ggggacctgc
    25681 tggtaggtgc cggaaaagtt gatccggctc aggatgtcct gggtcttcac cacgtgggtc
    25741 ttgatctcct ccctctgttt acccgtcagg ggaccgctct tcttgaggat cgagtccttg
    25801 atcccgatct tgccgtagtc gtggagctgg gcggccaccc ggatgacctc cgtgaactcc
    25861 gcgggcatgt tcatctcctc gcagatcgcg atggcgtact ccgtgacccg ctccgaatgg
    25921 cccgcggtca ggctgtcccg ggcgtcgatg ctggccgcca gggtgcggag gatggaccgg
    25981 aactgccgtt cccgttccac cgcgaagaag gcgctgttca gggtcaggcc gatctccggg
    26041 gcgatgccca tgaggaggtt gatgtcgctc tccaggaggg gccgcttgct ctgcaggttg
    26101 tccaccgcca ggactcccag gcactggtcc tcgaagagga tggggcagca gatgaaggac
    26161 ttggagccca gccttcgcag gaattccagg ctgtgggggg agatctccga ctcgatcgtc
    26221 gacacgtcgt tgaccaggaa gggcttgcga tcccggtagc agatgacgaa aaccccccgg
    26281 gagtcgggct tgtacaggct gaaccgggtg gcgcggatct cccggtagag atcctcggtg
    26341 tacccgaatc cggcccggaa gaccaggttg cgcttgtcct cgtccgccag gagtatgatc
    26401 ccccggtcgt aatccccccg ctggctcagg atctccatca ccctggccaa catggcgtcg
    26461 acgtccgtgg acgccgacag gacgtggccg atctccttgc ccatcagggc gttgttgtag
    26521 acccgctcga agtcctcgaa gctccgctcc gtggtcagcc gctgatgctc gatgaccgcg
    26581 ttcagctcgt ggaactggac ctgctccttg gccagggaca gggccaggac ggccgcggac
    26641 aggatggcgg cggtctcgag gaagcgctcc ccccctcggt acccggccgc ggcggccagg
    26701 atcggcacgc aggcggccag aaggcccagg cgggccatcc ggagcacgac ggagaaggag
    26761 tcgtcccagg agatccggta ccggcaagcc ttgccgccct tgaacacgca ctcgggatgc
    26821 tcgatccggg gtttgcggct gttgtagccg gacaggatcg cctcgaagta gccgatccgg
    26881 ttctcgcact gatagggctt ttcccggacc ccctcccggg gggttaccga aagctcgatc
    26941 tgcttacggc tcagggtccg ggattcgaag agggtggacc gggtcaggcg cccggccagg
    27001 ttgctgatga gctcgaaggc cttgcccggg ttggaaagcc ccaggacgta gtggtagaac
    27061 cggcccaagc cctccgggga ggcgctgtac ctcccggctt cccgggcgat tccggattcc
    27121 cccgtggcct tgacgacccc ctcgtagaac aggtccacct gggcctgggt gaaccagtgg
    27181 ccctcgtcct ccgcctggtg gggggccatc cccgcccggg cgagcagggc cgcggagtcc
    27241 acgaagggat actttcgctt caggagcctc aggtacgtgg agatgatctt gctgttgtag
    27301 agcggttcgt cgtcagtccg aaccggcccg tcgttcctct tgttcacggc aggatctcct
    27361 gaaggttccg cggacggatt cgaagtaggc gtccgagtac gcggtattca atacccagag
    27421 ggcgtatttt ttctcgcagg gcaccgaata cctgtccagg tactcctcgg ggtagaccag
    27481 gacgggcggg gtctcgtcgt cgtacgcgcc ccgcagggca tccagggcgg agaagagggt
    27541 gcccgccgga aggcgttccg ggtccagaac ccagacgtgc aggcagctcg tcaggctcga
    27601 aaggttcagg cccaggtcgg acacggtcac ctggatgacc gcggccaggg cgcccccgca
    27661 ccggaggctg tagacctgcc ggtcccgccg gaatccctcc cggcggtact ccccggagag
    27721 ctccgcgtcg gatccgtccg acttcgtcag ggccagggcg tccagggtca gtcccccgga
    27781 tcgggcctcg taccaccggc gcagctcccg gaagtcctcg ggacgtgcgg ggaagagctg
    27841 gaatcccccc ggggcccctt ccggcggggc gggcagacgc aggtagccga agatgtccac
    27901 cgaggagccc ttgggatcgg cgatatcctt ggccacgttt ccgaagaccc gggcggggaa
    27961 gcggttctcc gggcggtagt agcacatcag gtagtccatc cgggccccgg ggtggaggtg
    28021 gaagtcgttc gtgaaccggc ccgcctcgtc caggacgctc acccccgcgg cgccgtaccc
    28081 gtcccgggag gccgcgtggt gctggatcag ccaggaatcg ggatagaacc ggagcatgga
    28141 catgtgcccg aagatccgcc ccttgtcccg gaagaagaag tggcgggcta tggaggggct
    28201 gtcctggtac agcttccggt aggtcctgca gaactcgtcc ttgtgggtct ggagggaaag
    28261 gtatttggac gggtagagga agcccgagtc gaagaagaac tgccagagct cctccatctc
    28321 cacccggccg cacacggaca ggcggccgtc gatggaggca tggacggcgg aggagagccg
    28381 gacctggtcc cgggcgtcca ggtccaggaa cgccagtccg taccgggcgg tccgccgtcc
    28441 ctccggcttc tccaggacgt tcacgtagag gacctgggcg cggcaggccg ccaggacgct
    28501 ccccgcgagc tccaccgtca ggtcctcgag gatcaggccc gggaagaggg aggattgctc
    28561 gaagaactcc tccacgcaga cccccatgcc cgagacgttc tccgcccgga ggaaggcggt
    28621 gcggcccgag agggggtggg tgaatcggac cacgggctgg ggggagagct cccgtcggtg
    28681 gctccggtgt tcccggggcg ggaagcgctg gatgttcgtc cgggtcgggg acagggccat
    28741 gcgcttgccc ggggcgccgc gatccctcgc gaggaaggcg cattctccgc tgaacaggag
    28801 gatcccgtcc cggaagagca ggagcgtgac cggctgggag ggattcagcc agttccggga
    28861 gggggacggg ccgtcgtcca ggataacccc cagactgacg ccgttgaagt cctcgaggtt
    28921 cccttcccag gtgacgcccg attggaggac cttcgcccga actcccgcca cggggtaccg
    28981 ttcctggccc cggacgctct cctgggtgcc cggctcggga agccggagtt cgatgcccgc
    29041 ctcgtccatc ccggcggcct ccgcgggaac ggaggtgtag agggtgccgt cgctgatgag
    29101 cagtccgctg caggagtagg ccgcgagctt ctccggttcc gggccccggg aggcccagcg
    29161 gcatgatagg gtacggccgt cgctgggcag gggcagggct ttcagggaaa aactccccac
    29221 cccgtcccgg cgctcgaaac ggacgaagac gctgccttcc ttgaagtgta tccggttcag
    29281 gaggttggcc aggtcccggt gcttgatccg ccggccgccc cgaagatcct cggcggaggc
    29341 tgccccgacc tggaccgccg gcttggccgg gagtcccggg gggaggaagg gagccgagga
    29401 tcccaggccc ccgtcgtcat gcacggcggg cctcgggggg atggtcgggc ttccggtcag
    29461 tatccatcgg cggttcctgg aataatcgtt catcgcgctt catggtaatg atcggcggaa
    29521 tggacccccg gcaatacagg aacccgccgg gaacgccggg ccctgcttga cggagggcca
    29581 ggattccggc atcctgcctt cgttaacccg tatatggtca tatggttaac ctaaatcccc
    29641 gaaaaggagc ggctcatcac ggatcgaggc aagctcggag acaggctcat cctggaacgg
    29701 ctccgcgggg ccgaaggccc cgtatccggg gagtacctgg cccgggacct gggggtcagc
    29761 cgggtggccc tctggaagcg gatcgaggcc ctcagggcct ggggctaccg gatcgagtcg
    29821 agccgccggg gatacgccct ggtccgggac gacggtctgg ctccggcgga cctgccgcct
    29881 ccggtccggg tcctgtccga ggcgggatcc accatggacg aagcccggga ctggggaagc
    29941 gcgggggctc cctccggagc cgccgtcctg gccctccggc agagcgccgg ccggggccgg
    30001 ggcggccgaa ggtgggaaag cccggacggc ggtctttacc tttccctggt cctccgctcc
    30061 gccctgcccg cggcctgcgc gggggccctg gtcctggagg ccgcgcgctc cctgctggat
    30121 gccctggcga cggccggaat ccggggggtc tccttccggt ggcccaacga cctcgtcgcg
    30181 ggcccccgca agctcggggg gatcctggcg gaatccttcg ggggcctgga ccgccccgag
    30241 ttctacgtcc ttggaatcgg gctcaacgcc gcccccgtcg aggtatcggg ccgggcggcg
    30301 gcgggcctcg cggatctggc gccggggctg ccccggaggc gggacctggc cctgggcctg
    30361 tccgagtccc tccgggcctg gtgcgaacgc ccggacacgg atccccgccg ctgggcgggc
    30421 ctgaagccag acccccggcg cccggcccgc gccgagctat gggacggccg gatcctgggt
    30481 atccgtcccc ggggctatac gacccggggg gagctggccc gcgaaggcga cggccccgcg
    30541 ctggccttcg gggagtgccg caagatgacc tacgaaggag aggaaccttg aagaacagac
    30601 cctggaacct cgcggcgtcc gccctcttcg cggccctgat ctccgcgggc gccctcttct
    30661 ccctgcccct gccgccgcct ttgccccccg tgtccccggc ggtcttcttc gtcctcctgg
    30721 cgggactggt cctgggtccc gccggggcgg gggcttccgc ggggatctac ctgctcctgg
    30781 gctccctggg cctgcccgtg ttcgccaacg gggcgggggg actggggcac ttcctggggc
    30841 ccacgggggg cttcctggca ggatacctgg ccgcggccct ggtgaccggg gccctggccg
    30901 accggaagcg ctggggcgcg gggcgatcct tcctggccgc cgtggccggg gtcctggcct
    30961 tgtacgccct gggccttccc tggatgcggc aggtcctggc ggccaagttg gccaccctgg
    31021 ccgcggcggc ggccctgatg ctcccgtact tcatcgggga cctggtcaag gccgccgcgg
    31081 ctgccctcct cgtccgcgcc ttgaagcccg tactggagcg gcacttcccc cgggaggacc
    31141 tgtgaggctg gaggcccgcg gcctggtgcg gatcttcccg gacggcaccc gggccctgga
    31201 cggggcggac ctggagctcc ggacgccctg cttcatggtc ctgtccgggc ccaacgggag
    31261 cggcaagacc gtcctggccc ggcatttcgt gggccttgag aagccccagg ccggggccgt
    31321 cctggtcgac ggggtccccg tggagcggtg cctggcggag acccggcgcc gggtgggctt
    31381 cgtgttccag gagccggagc accagatcct ggggatcacc gccggggagg acgtggagtt
    31441 cgggatccgc gtaagagtcc cggaccgggg ggagcgccgc cgccgggcgc tccgggccct
    31501 ccgggacgcg ggcctggccg ggtcggagga tcgcctggcc tcgtccctct cggggggcca
    31561 gaagcgtcgc ctggccgtgg cctccgccct ggtctcggac ccggagctgg tcatcctgga
    31621 cgagcccttc aacggcctgg acccggcggg ggcctcgggg ctcctgcgga tgctcctttc
    31681 gctccgggat cggggagtgg ggatcctcct ggtgacccac gacctggaga agtgcctggc
    31741 ccacgcggac cgcctggcgg tcctggactc cggtcgggtg gccgcggacg gccctcccgc
    31801 ggagctctgg gaccggcttc cgtccctggg cctccgacgg ccgccgggag ggtccgggcg
    31861 gctggaggag atgacatggc tggattgaac ggccgccccg ccgccttccc atcggactac
    31921 cgcgcccgct tagccctcta cctggccctg caggccgctg tcttcctggc ccccgggccg
    31981 accatcctcc tgcccgccgc cgtcgtcgcg gtcctgggag cccgggcggg ggtggactgg
    32041 ggacggtggc tggtccgact aggtcccctg ctgggaatga tcctcctgcc cgccctggcg
    32101 ggcttgccgg aggcctggcc ggttcgggcc gagggagccg ccttcctggc cgcctggtcg
    32161 ccgggtctgc gaaggagcct ggccttcctg ctggtcctgg cctcggcgga atggctttcc
    32221 cggaccaccc gggtcgacga aatccgggat accctggaag gcctgctcgg ccccctggga
    32281 ccccggggac ggcgggcggc tctggccgcg gccctgaccc tgggattcct gccctgggcg
    32341 cggtacgagc tggcccgggc ggacgaggcc gcccgcctcc ggggttcgga cccccggcgc
    32401 ggtcccctga ggcacctggc cgtcctgggg atacccctga cggcccgtct gctggaaaag
    32461 gcgcgcctaa gctcggaggc cctggccctg cgggatgcgg atccggaccc gggcgcctcg
    32521 aaccttgcca ggagcccccg caggttctac agttagggga taggagtctt ccatgcggat
    32581 atcgcgcttc ctggcggccg ggatcctcct gctcgcctcg gcgtccgccg cgggccagac
    32641 ccggcgggtg gctgtcctcc ccttcgagaa cgacggcctg gcggcgggcg acgtccgcgg
    32701 cctgaccctc ttcttcgaga cggccctgca gaacaccaag ctctgtgaga tcgtggaaca
    32761 gaccaaggtg gaggagatcc tcaaggccca ccagttcgtc ctggcggact tcaacgaccc
    32821 cgccaaggcc gtggccatcg gcaagctcgt accggcgaat acgatcgccc tgggcgccgc
    32881 ggggaagctc gggggccgtc cctacctgaa cgtcaagctc atcgacctgg tgagcggcac
    32941 cgtggtggcc gcccgcaacg tgaccgccgc cagcgtcgag gccctgacct cggaattgaa
    33001 cgccctggcc gcggggatca tgggcagtcc gtcctcctcc ctcgcctcac cgcccgagac
    33061 ctcgcgcccc ggacctcccg tacccctgac cgcggtgccc agggtgggcc gctcccagct
    33121 ctcgatgttc gagagcggcc cgggcatccc cccgccggaa tcccggatct acgccgtgac
    33181 cttcttcacc gcctacaccc gcttcatcaa ctgggacctc ttcctggagt tttccgcccc
    33241 ggtatcgcgg agcatgaccc tgcccgtgga cgcgaccata ctccgctcca acggcacggt
    33301 gctcacggtc cagagcctga cggcgaacct agagtccggc tggaactggt tccggatgtg
    33361 gaacggctgg ggggctccca acccgtacaa ctgggctccg gacacctaca cactccgggt
    33421 gaccctgggc ggagtgcccg tggcgacgac caccttcgtg gtgcggtaag ctccaagaag
    33481 gcaccgcgga ccgggcggac ggctgccgct tgcgccggaa ctcgaaacga ctacacttga
    33541 agacgacatg tccgggcccg ccccgagggc gcccggtgta tcccatcttc gatccatgag
    33601 gagcagacca tgaaacgctt cgccctgacc gccctcatcc tggccctggc cctggccccc
    33661 gccgcggccc agggcatcga cctaggcaac ttcccgctgg gaagctggct ggacgccaac
    33721 tacgacgccg tatgggagtt cagctccggg aacatccgca tcctgtcccc cgccggggac
    33781 gtcctcttcg acttcggcgc ggcgggcgtc cagaacttca aggtcggtgc ggggggcgac
    33841 ggtcccttca tcaccttcgc ctgcgaggcc gccgggcgga cctacaagat caccaagccg
    33901 ctgaccaagt cctcagcggt cctggagatc cagcgcccga acctcgcgga ctacaaggtg
    33961 gagatgccga agaagtaagc cggcgcgggc ggcccgccga ggagggggcc gccgcttttt
    34021 ccggctccac gttgcgcccg cgtcgatcgg gtgatacgat ttccccgacg tcgcggcggt
    34081 tcccgcccgg ggcggccgag acgatccatc cggaaaacaa aggagaacgt catggccgcc
    34141 accaagaaac cctcgaaggc acccccagcc tctgcaaaac cgaagaccgg caagaagccg
    34201 gccgccgcgc ccccggtaag caaagccgcc tcgaagacgg ccaagcccgc gccgaaacag
    34261 tcggccaggc cgaccgcaaa gaaaccgacg gccgcagcat ccaagaccgc tccgcggaag
    34321 ccccttccgg aatccgtgat cccgatcccc ggcactgccc cggacgtacc cgcccagatc
    34381 gtcgcgttcc tggagaagtt cgacgccctg aaggccggca agacctgggc cgtgaaggcc
    34441 gcagccctga tcgagaagat ccccgcggcc ctgttcggcg agttcgagga tgccctggac
    34501 ctggagacgc ccctttccac gggcaagatc cccctgcacc tgatcgaccc taaaacggcc
    34561 aagacggccg ccgccaagac cggcaagggg acgatccccg cgccccggcc ctcccccaag
    34621 accttcggga ccgccatcct cggaaacaag atcggccggg aggagttggt ggaactcgtg
    34681 gagcgcctgc gctggtgcct ctgctgctgc cactgctggc cctgccgacc ctggcccttc
    34741 tgcaggatct gccggtggcg cctggtcaag gtgagcagca cggcggattc cggctgggtc
    34801 tatcacctca acttcaagaa cgcgtcggcc gccatcgcgg accgggtggc ggaaatctcc
    34861 tgcccgtccg gaggatcctc gggcggcccc tgggctcccc aggtctggaa cgcgtcgacc
    34921 ccggccgccg aggccccggt cggatatgcc gaggcgggcc acgatcacga cggggtctac
    34981 tccgccaccg ggcacaacca cgacgcggcg tacgcgccga aacagaagag cggggcggat
    35041 atcaagtacg ccgagatcga cgcgcccgtg gggcagaccg tcaagttgag ggacaaggct
    35101 acatcgccct cccccgcgga cgtgacggtc agcctgtcgg ggcacaacca tgatacggcg
    35161 tacgcggcgg ccgggcacaa tcacgatacg gcgtatgcgg ccgccgggca caaccacaat
    35221 acggtgtatg ctgctgccgg acataaccat gactcttcct acgcggcagc cggacacaac
    35281 cacgattcag cgtacgcggc ggcgagccac gacaacacaa gccatacgaa gaagtacttc
    35341 tcccgaacga acctgatcat gaaggcgaac ccggacacca cgttggacgc gggacactac
    35401 tgggttaaga ttcaactcag cttgccggtg gggtttgccc cgggcacgag caattgggcc
    35461 gagtgcgtct ccggggtatg tatggatccg accatggccg ccgcccccct ggacctccaa
    35521 gccgtttcgc cgacgatcat cacggagttg gagggcggag taaacgtgcc caagctcaag
    35581 ttccgcctca cggattccgg cggaaccgac taccggaaaa acaacggtat ctgggcaggc
    35641 aagctcgtcg tggttaccgt ggcggcgccc gtcacggact gaatcccatc agggggtgaa
    35701 acggtagatc gtattcaccg gtggtgagga catggaaacg agtccgccgg agacgtagac
    35761 ataccccccc gagctcgcgg ccgcgggata atccaaggcc gcgggcatcg tgaagtcctg
    35821 ggactccccc tccgaatccg agtatttcct taccgcggca gagataccga ttacataccc
    35881 tccgaagagg tatacgtttc ccaggtcggc ggcagaggcg aatcggtacg acgaagtagt
    35941 cagatccgta agatccgata tggaagggcc ggacggatcg aaggagccta cgtccttcgt
    36001 cgacatggat ccgccgacaa ggaggattcc cgctccaatt gggacagcct gaaagtaggt
    36061 gcggtccgag tacaatcccg acgaaagtgt aacggcggcc ggatcggacc ccggtgagta
    36121 tgcgtagatt gtatctaagg aaccgacgcc actgtctccg gggaatatgt agatgacacc
    36181 gccagaaacc gcagcgcaaa ggttgtctat agccacgggc agctcgtcgg tgacagttgt
    36241 ccagttcccg gggttctgcg tatcgaatac ctcgattgaa gcggaaggga cgaggacagg
    36301 atcagggtca gggacaccat taacagttcc cccgatgaca tagatttttc tgttcaatac
    36361 cgcgattccg aaagccgcgc gggcagagtt caaatcatat ccaagatcgg aaacaacccc
    36421 ggtaccgaca tcatacacct cgcagtccgc cgtcatgagt ccgaagcttt gatctgtgaa
    36481 gcccccaatg atatagatct tcccatccac ttcaacggcg ccgtgcccca tccgggctgt
    36541 cgtcaatgta gccgacatct ttttccaggc gagttcgaag gtccgggatg cgacagacga
    36601 ctcccgcagg ccgggtttaa aagcacgcgc cttgacggtc gtcgcatcct ccgtcccggc
    36661 caggttgaat ggaacggtgt attggtaatt agtagccgaa accgtattcg taggggtgct
    36721 tccgttcagc gtgtagtata tatccgcgcc gccgttcgga tccgtgatcg tgacggatcg
    36781 gcctcgggca ggagtcatag agtatctaaa gtccggttcc ccgttgctta tcaccggcgg
    36841 ctggagtgtc tcagtctcca cgttgatgtt ctggcaggaa agcgccgcgg ctaggagggc
    36901 gagggcggcg agaccggacg cgatgcttcg agtcctcatg gcttgcctct tttctcggat
    36961 tgttacgaca attctatccc atcccggggg attttgcacg aatcccgcgc aggatgccga
    37021 cccgccgcat ccatcctctt taccctcgac ctacgccgcg aagaccggcc gaggtccgta
    37081 ccttccgaaa tcggaccgtc gagagttagt ccgccgaaac gagtcctgta cgatttcccg
    37141 ccgaaacatt gcgcccgcgc ttgaacagtg ctacgattct atcgacgtcg cggcgcaaac
    37201 gcccgggggg cggcccgaga cgatccatac ggaaaccaaa ggagatgttc atggccgcaa
    37261 gcaaacccgc gaaggaaccc gcagccgccg cgaaacccaa gaccgagaag aaaccggccg
    37321 agaagaaagc cgcggcgccc aagcccgttg ccgtcaagaa gccggctgcc aagcccgctg
    37381 ccgagaagaa gcccgcagcc aaaccggccg ccaagcccgc cgccgccaag aagcccgcag
    37441 ccaaaccggc cgccaagccc gccgccgcca agaagcccgc agccaaaccg gccgccaagc
    37501 ccgctgccgc caagaagccc gtcgagaaga aagctgcggc tcccaagccc gcggccgcca
    37561 agaagcccgt cgccaaaccg gctgccaagc cgacgggaaa gtaagttccc caggcaaagc
    37621 cgggtgcccg cacggacccg gctgcctgta tccttgacac ccgcgccccg atgggcgatc
    37681 ctggatattc ccgacgacca cgctcgggta ttcggcggcg cgcaagccgg tccggcgtgc
    37741 tcctgaacag agcgccccgc tcggccgccc gcgccgttcc cggaggttcc ccgtggaccg
    37801 acgagatttc ctcaagaccc taggccgggg cgcgggcgcc ctgggagccg cgtggctcct
    37861 gggtcccgcg tcccgtgtct tcgcgcaggc cggcggaacc ccgacccccg gcgcctaccc
    37921 ggacctcgtg gccgtgaagg gcggaagcgc cgcctcccgt gtggacgccg ccctagccgc
    37981 ggccgggggg gtctcccggt acgtccgcaa gggcgccctc gtggtcatca aacccaacat
    38041 gggctgggac gtccggcccg aactcgcggc taacacggac cccgcggtgg tcaagcggat
    38101 cgtggagctc tgcctggccg cgggggcccg gaaggtcgtg gtcttcgacc acacctgcga
    38161 ctcctggagg gcggcctacg cgaactccgg gatcgagcgc gccgcgaggg acgccggggc
    38221 cgaggccgcc ccggcccacg cggaatccta ctaccaagcg gtcgcgaatc ccgcggcccg
    38281 caccctcggg caggccaagg tccacgagct cgtccttcag gcggacgtct tcatcaacgt
    38341 cccggtgctc aagaaccacg gcggggccgg gatgacctgt gcgatgaaga acctgatggg
    38401 cgcggtctgg gaccggggat atttccattc ccgcggcctg gacgcctgca tcgccgaggc
    38461 ggccctcctg cgcaggccgg acctcaacat cgtggacgcc ggaaaggtca tgctctccgg
    38521 gggaccccgg gggagcgcgg cctcccggta ttccgaacag cgtctgctca tgctttcgtc
    38581 cgacatcgtc gccgtggatg tcgcctccgc gcgcaccctg ggacccgggc ccgacgcctt
    38641 cgagtacatc gcgaaggccg ccgccctggg cctgggtcag ccggacctgg ccaagctggc
    38701 ggtgcggcgg atcgagcttt gaggatcccg aacccggccc gcctcgcctc ccggatccgg
    38761 ggcggactgg ccgccctgtg gctggccgcc ttctgcgccg gggtcgccct gcccgccacg
    38821 gagcttcccg agaccgcgcc cctggcggcc ttcctcgccc gcacccagcc cttccacgcc
    38881 ctggagaaga tcctgctcgc ccgggccgtc cccgccgccg ccggggcctc gatcctggcc
    38941 gtcctgacgg tcctggtcgg caccctggtc ctcggccgct tccactgctc cctcacctgc
    39001 cccctgggaa cctgccagga cgcggcggcc cggctggcct cgggcctccg gtccccgggg
    39061 cgcggctctc cccgaccggc ccaccgcagg cttcgtaccg cgataccgag atggacggtc
    39121 ttcggcctgg cgatcgccgg gctcgccgcg ggcattcccg ccgccgcggc cctcctggag
    39181 ccctacacga tcttcgcccg gggcttccag cggagcttcg gggacctggt cgccctgggg
    39241 ctcaacggac tggcggccct gggagcgcgc atcccgatcc tgacggtacg atgggagccc
    39301 ttcgccttcg ccctggcggt cctgcccttc ggggcggtcc tgtcggccgc ggccttccga
    39361 agccgcctct tctgcgggga actctgcccg gcgggcaccc tactgggtac cctgaaccgg
    39421 gtggccctcc tcagggttcg cctggaccgg aaggcgtgca tagattgcgg agcctgcgcc
    39481 cgggtctgcc ggacccgctg cgtggacggc cggaccctgg acgcgagccg ctgcgtccag
    39541 tgcctggact gcgtggccgc ctgcccgacc ggggccctcc ggtacggccc gccgcggcgg
    39601 gcggccggcg tggacgcctc ccccgaattc gcgccggacc ccggccggcg cagtttcctg
    39661 gccgaggcgg gaggcgcggc gctcggcgcc gccgcgggca ccgcccttgc cctggcggcc
    39721 cccggtctcg ccgggacggc gggatcgccc ctcgtccgga tcgagccccg gggcgcctcg
    39781 ggccgcctcc tggccgcgcc tcccggggcg ctctcggtgg accgcttcgc cgggctctgc
    39841 accgcctgcg gtacctgcat ccgggcctgc ccctcggggg tcctgaagca ttcaaccgtc
    39901 gagttcggac tcggccggcc cggggttcct tacctcgact acggccgggc cttctgccag
    39961 tacgagtgcg ccgcctgccc ggcggcctgc ccctccgggg ccctcctgga ccgcgacccg
    40021 gatctcaaga agcgggaggc gatcggcaag tccgccctgg tcctggcgga ctgcatcgtg
    40081 gtgtccaagg gcacccgctg cggggcctgc gccgagcact gccccaccgg ggcggtccgg
    40141 atcgagcggg tcgaaggccg ccccctcccc gtcccggtcc tccacgcccc cacctgcatc
    40201 ggctgcgggg cctgcgagac cgtctgcccc tcgacccccc ggaaggcgat gaccgtctcc
    40261 ggcctggctg tccacgagat cgcggatcca ccccggaagg agccccccgt gcccgtggag
    40321 catcctccgg aggaaggctt cgcgttctag gcggcatccc cccggagacc cggggcgaag
    40381 ggaacaccgt ccctcctgca catcgcccgg atcgcgttcc ggcccgactg ggccgcggtg
    40441 gtgatcccgc cccaggcctc cacccactgc cccgcccgcc agaaaccatc gagcccggga
    40501 tcgtcgtacc gggccgcccc ccgcaggagg gtccggatca tgccggcgtc gggcttccgg
    40561 gcctgcatgg tgcccatgta gtttccggtg taccgctccc gggtccgggg cgtggacacg
    40621 tccgtcacct cgacctttcc cgcgaacccg gggcggaagc gctggaccgc ctggacaacg
    40681 atgtccgcgc agcgtcgctt ctccgcctcg taccgggctc gatccgcctc cagatctttc
    40741 cagaacccgt agtccgattc caggaaggcc atgagggcgg acttccccgc gggagccgag
    40801 gcgggatcgt agcagcggtg gtggaccgtg agccggtgct ggggccgtcc ggccgcggac
    40861 ggagcgtccg ggagcagcca ggtcaggggt cccgggtatt ccgaccagtc ctcggctacc
    40921 ccgaggttca cttggacggg ctggtccgac atgcgccggg ggtcgaactc cgcgccatcc
    40981 gagctccctc ccgggaggag cgagcgggtg aagcgcccgt ccgcggcgga caggacccgg
    41041 tccgcgggaa attcccggcc gtccgccagg gcgacacccc ggaccttccc ggactcgatc
    41101 ttcagccgca ccacccgggc cccgtacagg acccggccgc ccagctcacc gaggcgggcc
    41161 tcgatcgccc gggcgaagga aatccagcct ccccggggga tcccggtcat cctccggtgc
    41221 gcgtaggcca agggcaaaag gacggtcagc aggggtacgt cggtcccccc gaagtggaca
    41281 aggttgccga aggcctccac cgcgagggga tccgagagcc cctccaggaa gcgcctcagg
    41341 gtcagggtcc cgtaccgcag gaggacggga agggtcgaca gggccgaggg tccggctccc
    41401 cggggctctc ccgccagggg catgtcgaag cggaggcagg ccctgagcgc cccgacgaag
    41461 gcccggaccg cccgggcgtc ccggggaaag ttatccagaa aatgcgcctc gaaccggtcg
    41521 atgtccgtcc atactgtcac ggtcctgccg tcgctcaacc ggacgctccc gaagctctcc
    41581 ggatcccgga ggggacaccg cccgatcacc ccgagttcct tccacatccg gtacaggggc
    41641 gccccggggc ccgagccggc cagtccggcc gcggacccgt cgaaggtata cccgccccgg
    41701 ctccaggtgg cgcagagccc cccgggagtc cccgccatct ccaggacggt cacccggtag
    41761 cccgaggccg cggcatagac tcccgcagac agtcccgccg tcccggcccc gatgatcagc
    41821 agttcatcgc tcattcgatc catccttccc ctcacggcgc gcctcgcgtc gccgggcgtc
    41881 ccgctccgtt ttaacaagct tcgcaccacc ggtacccgaa tccggaattc ccagcaggtc
    41941 gcaggccttg gccagcaggc ggtcccgggt cttcttgtcc cgggcatccg ccccgagcca
    42001 ctccgcggcg ccctcagggc gtactagggt catgtgcacg aaggccagga gggaccagac
    42061 ccggaaccgg gcctccgctt ccccgaggcc gggcgactcg gaccggagca tcgccgccag
    42121 ggcggacagg gtctccccgg tcaggctctc gccgcctccg cccgattcca tctcgctctc
    42181 cagggccagt cgggcggcca cgggctcgtc gaagaggaaa tcgcagatcc gcgccaggtt
    42241 cttccggagc cccgggcccg tggatccggg caggatgccc cgctcccggt agtattcccc
    42301 gatgacggcc cgaaccgcct cccggatcag ggcctccttg gagccgaagt ggtagttgac
    42361 ggcgttcagg ttgacccccg ccgcctggat gatccgccgc accgtcagat cccggacgga
    42421 tccggctttc cggatcaggg ccgccgcgga atccaggatg gactgccgcg tcgtggatgg
    42481 ttcatatttc atactttgtt tcttacattg tttcataaaa cctgtcaagg gatcccgatg
    42541 gaggcggaaa aacgagcagg gcgaaagccc gggatgtgcc ggatcaatcg cggatcgtgc
    42601 ggtccttcga cacggccccg gggattcgga cgggagcgaa actggagacc agcttgtggc
    42661 agtagaggac ggggaaccag gccagatccg gacggccgaa ggccaggacg cccgcggcgg
    42721 atccgagggc cgccagaccc gcgcccaagg cgtcctgcca gggcttcccg tccgccagga
    42781 tcacggcggt cgcgaagacc gcggacactc cccagagggc caggggagcc ctcggtccga
    42841 tctccgggaa ggcggcccag agcaggagcg gctggaggat gtggattccc aggaacgaga
    42901 accgtagcgg gcggcctccg cgggcataga agcggtcggt gctcaccgtc cggttcgcgg
    42961 ccacgccgcc caggatgtcg gccgccagca ggaaaccgac ggcccgctgg acggggctcc
    43021 agccccacgg actgcccgcc agggccgccg cgaccccgcc ccccaggccc agggccagga
    43081 ccagggccac ctccggcttc cggggttcct tcccgaacag gaaggccatc gccgttccga
    43141 accgaccatt catggtatcc gccttcgccg tcccggccta cggcccgatc ttcggaaccg
    43201 tcacgccctt gaggaggtcc gcgcccaggg ctttcgaccg ggcctcaatc tccgcccgct
    43261 tgccgtccag ggatttttcc agggatgcgg cggaagcggc gtccccctcg gcggccttga
    43321 acagggaatc gaagtccttc ttcggggcga gcttgccctc cagctctccg tcgatgtacc
    43381 ggcgaagggc ggcgtcgagc tcccgggaag cctgggcggc gtaccgtcgg cccgcggcgt
    43441 ccacggcccc ggcaatgatc cggtccaggt ccgtgcgcag gacgaaggag tccttcccgc
    43501 ccgggacgac ccggtactcg gccgtaaggt tcacggcccc ggcgtcccgc agggcgtccg
    43561 cggccgtctt gcccagggtg cccgaggggg ccgccacgac ggggttccgc acctccgcct
    43621 ccagggacgc agagaagccc ccgtcccgga cgccccggac ttccgaggtt ccctccagga
    43681 tcccggtgag accggaaagc cccgccccgg caagggagtc acccaggtcc aggggaaggt
    43741 gggccgcgct cagcgaagct tcccaggatc cacccgcgga ttccctcaag tccgcccggg
    43801 cgtccgcccg gaccagggag ccgcccctcc ggatctcgat cttgagggac gagggtgccg
    43861 gcaccaggtc cggttccgtg gagacctccc ggacctcgac gtaccacgcg gagtccccgg
    43921 ccttgaagct ggaagtaagg agccccaacc ggaaccgggg gaaggcggct ccggggaaga
    43981 ccacgtcccg gccccggaag gcgggcttcc cgccggcctt gtcggcgccg gccgccccgc
    44041 cccccgagcc cttgagacgc gacgccgcct ccaggaacct cccgccgtaa tagagccagg
    44101 tttcaccctt gtccgtgagc agggagcgga gcgcgggctc cagggcggcc cgggcggctc
    44161 cgctgccggg ggtgaccagg gacttcaggt aggccgcgtc gcggtccgcc gtgtcccggg
    44221 cctccttggc ctggcggtcc agggccgcgg tgtccttccg gatcccctcg gccacggcct
    44281 tggcctccgt cccgacggac tggacgtccc cggcggccgc cttgaccacg gccagggcct
    44341 gggccgcctc cccggcggtt cggattgctt tcacgtcgat ggccagggcg gccgtggagg
    44401 aggcccgggc cttctccacg gcggcggagg aggtttccct ccgccccttc cactcctcca
    44461 ccgaccgggc gtaggaccgg ccggccgcct cgtaggccgc gggggcggcg agcttggcct
    44521 tttcccgctc cagcagggcc tgggcgtcga aggcctccca gtccaccagg gccgggatct
    44581 ccggggccgg gcgcttttgc cgaggctcgg gctccgccgc gccgggcagg gcccccgagg
    44641 tcgagcgggg cgccccgagc tcgacggacg ccgcggaggc ctcctcgatg tagaccttcc
    44701 cccgcaggac cgcggagggg gagaggcgga actcgagccg gcccgcgcgg aagaggtccg
    44761 tcatgggaga ggccgcgtcc gccaccgcga cggagccgac ggacaggcgc atccggaagg
    44821 gccggaaccg gaggccctcg accgttgccc gggccccgaa ggccgcctcg agcccggcct
    44881 ccgcgccgga ctccaggagg gggttcatga agaagatccc gaagaccgcc aggcccgcgg
    44941 ccaggaggac gagacccaga aggggcccgg ccttcacgta cccccggttc gccttggcct
    45001 ccttcgccgc gcttttggcc gcggccgccg ccgaccggac cgacttcgcc tcggccttgg
    45061 cggccgcccg ggcggccttg tcgcccgtcg cgttcgaggc gggcttcttc gcgttcttgt
    45121 ccatatccgc cccctattcc gccgaaccgg cgatcccggc cgcggccccg acggccttgg
    45181 ccagggcgct caccaggggt actttcttca aggccgtgac gagcttgctg cccgcgatcc
    45241 ggggggctat ccgggcccgg tacgcggaga cccccgcccg ggctgccagg aaggcgggcg
    45301 cccacaggac gatacccgcc aggaggcctc ccgtcaccag ggtgttgttg aaccgcgtga
    45361 ggggcgccac cggcatgccg tacagggccg tgaaggcggg ccgcaggacg ggggcgttca
    45421 ggaccgtcca gcccagggcg tccaggggcc ccgcggcggc cggggcggcc agcttgaaga
    45481 ccgccatgga aaccagggcc atgccgtaat gggccttgga caggaagagg agcaggaaga
    45541 gcaggatcca caggaggttg cccgcgggca ggagggccag gagcaggccc gatgcgactc
    45601 ccgcggcgat ctgtacggga ttcgtgttgg aggacagtgt cacgatcacc cgggcgatca
    45661 gtttcaggaa catgggcacc tccgattcgg atgaaacggt tcgggagcga gtcggaacgg
    45721 gaaccaatac aagtatgagg tccgggatcg acgaacgcaa caatcgggaa aagaacggcg
    45781 gccccctcaa gggccgccgc gcctgtctcg accatcggag gcggccgtcg tattgcgccc
    45841 cgcccctctt acctgagtcc tgactcctcc cctacagccg accctcgatc cggcccttca
    45901 ctacctccag gacctccggc agatccatgc cgatcttttt caggtgctcg ggggtcgggc
    45961 agccgtccgg tgtccagccc cggcgcttga agaccgcgtc caggagggac tcgtactgct
    46021 tgaggcggta ctcccgggtg atcttgacct tctccgccac ggtcttgccc gcggggtcga
    46081 tcccgatctt ctccttcatc tgcttgtcgt agcgctccgc ccgggcctcg tactcgtcct
    46141 cgaacacggg gcccatggcc cggtagggcg gcttgtcgtc cttccggtgc ccgaacccct
    46201 ggcggatgtt gaaggtccgc tggaagttgt agacccgctc gctctggagg atgagaccct
    46261 cccagtccag gggctccccg gtgatggcgg tgtacaggtc gatgtagttg tggacgtgct
    46321 cggggacctt gttgggctcc ggccacttgg cgttgtccgc gggctcgacg tcgttccagg
    46381 ggagcttgca caggccctgg aggccgaacc aggtccggaa catggggaag tagtgcagcg
    46441 cctcggcctt gttctcgaag gtggggatgc gattgttcac catgtccatg aagatcagcc
    46501 aggcctcgtc gtgctggggg cccttgttgg tcatgtagta gccgccctgc tgggccaggg
    46561 actccttgga gatgtattca ctctgctcca ggcccttgcc gtggagggcg atgtccttca
    46621 tgacctgggg atcggctccg tactccttgg cgaagtaatc cgccaggaac ttgacacccc
    46681 tgcccgccag aaccccgaag cgcttgccct ccgccatgtc gtggaggatc tggcaggccc
    46741 ccttccagtt gccccaggac aggtcgatcc cgtcggtgcg ctccttgttg aggaccccga
    46801 gctcccagca ctccatgagg aagctggtca tggtcccgaa ggagatggtg tcaatcccgt
    46861 aggtgtcgca gtagaagttg atctccagga taccgtgggg gtcgtagacg tagaggttcg
    46921 agcccacccc cgcggcggtc tcgtactccg gcccgtccac gcagaccttg gtgcccttgt
    46981 agggaccggt ctccaggagg aagtggtcca ccgcgtggg
//
LOCUS       DAOTEI010000004        41236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_642_length_41236_cov_5.612515, whole genome shotgun sequence.
ACCESSION   DAOTEI010000004 DAOTEI010000000
VERSION     DAOTEI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 41236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41236
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_642_length_41236_cov_5.612515"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..737
                     /locus_tag="P5313_00700"
     CDS             60..737
                     /locus_tag="P5313_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRY78909.1"
                     /translation="MIETLTPGSGVQRSLVRAATLLAGVYVAAQMLADIASLKIAVVG
                     GLAVDAGTFIYPITFTVRDMIHKRLGKSAARTVIVLAAGINLAMAGFLWLVSVLPADA
                     AWSAWEGANVTVNDAFARVLAPAWTIVLASIAAEVVSELIDTEVYHLWTSRVTSRFQW
                     TRVLVSNAASVPVDSLIFSWLAFGWGFGMPAPQVWQIFWFNVAVKGAVTLASLPGIYL
                     VRERGVQ"
     gene            1081..2259
                     /locus_tag="P5313_00705"
     CDS             1081..2259
                     /locus_tag="P5313_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY78910.1"
                     /translation="MDILKKCREFDEHKKVMALGLYPFFQPLQGTEGTEVTMSGRKVI
                     MLGSNNYLGLTFHPKVKAAAQKAIDTFGTSCTGSRLINGTLCIHEELEKRLAAYMKRE
                     AALVFSTGMQTNLGTISCLIGRNDIVITDKDDHASIVDACRLGYGEIKRFVHGDLDQL
                     ERILQKIPEENGVLVVVDGVFSMGGDIVDLPRLGPLCKKYGARLYVDDAHSIGVLGGG
                     RGTAAHFNQYGDVDLTMGTFSKSFASLGGFVVGDADIIDYVKHMARSFIFSASLPAPN
                     VMAAMAALDIMENEPEHVEHLWENARYMMKSYRELGFNIGATQTPIIPLIIGDDMKCF
                     AFWKEAYEGGVYANPVIAPAVPQGMALLRTSYMATHTRDQLDRALGIFEKAGKKVGVI
                     "
     gene            2632..3918
                     /locus_tag="P5313_00710"
     CDS             2632..3918
                     /locus_tag="P5313_00710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY78911.1"
                     /translation="MDPRTIALLMLISGVECSLLFFASGIFRKRTDGGAGRPSLFWGA
                     GTTVHVLGVLGLMTQGIVGPWFSVVFANSAIILGQLLILVGIRRLTAINPALPRYASA
                     WLLYSAAAVVFTFVLPSTTVRIILFSAAISAFYIEAAILVLISKSWKREPLTVYLTGT
                     FLAMAGFFAARALVTVFVSPASIFSPSAMNLATYIVSHVGLIGWSLGLILLQQRRTEA
                     DLERALREKEVLFRELQHRIKNSISVIASLVSLEASRVEDPGASAVLEGLEGRISAIA
                     SLYEHLFQSGQTDHVDLDLYLKAVVETLFSGQAARERGVRLNLEAQELRIDVRRAIPL
                     GIIANELASDCLKHAFPEGRSGTVRVSLRAEGPSAVLEVRDDGKGLPEGFTIGSSEGL
                     GLVLVDMLAKQVGGSFTAASEGGSVFTVKFPKEWKS"
     gene            3950..5029
                     /locus_tag="P5313_00715"
     CDS             3950..5029
                     /locus_tag="P5313_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRY78912.1"
                     /translation="MKTEGNPIRAVMDLFDDRIRREAADRFGMDGDSLEELEGSAYVF
                     AGERRDGKGRVRGILKITPGLRDPGPIMGASRAELEAEVDYVRYLAANRVPAARSLPS
                     LTGADVEEIPIDGDRSFLAYCFEFAEGSMFPDDDLTVFPPGVLREWGRLLGMTHRLAP
                     GFRPRPGARRPDWRGDDHLRSRLFVPASQEAVHRRFDETVLLLESLPAEPREYGIVHG
                     DFHHGNFLAEGERLTLIDFDACRFTWFSMDLATALFNCLPMPRSRTALRRDCAMDWLS
                     RILEGYREERTDSPSLLADLPLFLYVNELLAYSYRYKHWEPEELEDRADYLSSIRERI
                     EDRAPVVEFRPGDLESLAEPRRSMR"
     gene            complement(4924..6309)
                     /locus_tag="P5313_00720"
     CDS             complement(4924..6309)
                     /locus_tag="P5313_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRY78913.1"
                     /translation="MRAAEPFLALTEGLKVHFLVSAACLAAGLPTPPASSAAILFLAY
                     GTGRILGSLGGPVLFHALARLAAFSAAFLGFSVFAEYPSLPELSSALAAGTGWVRPAA
                     ALSCLGLHWIRGSWLERRKSDHAFHVARFDEGIGVFLVVLSIAALVGGELGRPAAAAL
                     PFLAFSILGLGLARREGDDPGAFSRRSALASLLAVSALFLGAAAGIALLMPLLTEPAA
                     RAGEALAEAAPAVLRLIGRLLGRIYRLETAAPRLSEGGSGILPGEAGDPEGGTLTGIF
                     VAVLGGIAALSAAALVVAVLVHLVRRLASPAERRNRPPDLSFLARWLSGIPAALRRAL
                     ARLGTLRSRRTSSPALKAYARLVSVGRLVGLARNRAETPREYARRLAAVFPRNAAEAE
                     FVVDRMEREVYGGKPRDPDADRRLAAARRGTRVRGYLMERLGSARDSRSPGLNSTTGA
                     RSSIRSRMDDR"
     gene            complement(6306..7541)
                     /locus_tag="P5313_00725"
     CDS             complement(6306..7541)
                     /locus_tag="P5313_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY78914.1"
                     /translation="MAEESRTPYLGSLFGTGGMGILLALLTAVAAAGRAYPLARLSAV
                     VLILMLALRAWTRIGSARLSASFELGNRRLFAGESSELGAEIVNRSPLALRLRLELPK
                     PAGIEATRASCLTAETSLLPFERRRGSWAFTAARRGIHSLGPVRLSAGDPLGLFTRER
                     ELPFPDELVVFPRILPLALPDLPFREYFGIRAARGIVEDPAWYEGTREYSGNRPARNI
                     HWKASARLAALQEKIFQPTSHRKVFLLVEGELFERAGDGEGFERALQIAGSLASLLSE
                     SGASFALATDRAVRDFPAALDLGRGPEHLGAVLELLARCGIERGRDFRSLLAGTVPGN
                     TGFLVLARSPGGGAGAALAGTAGRRDPVLFLYAEPAGGGEPESTTPADHRAPPADRAA
                     PAGISLRFTDLLPPEDNES"
     gene            complement(7549..8490)
                     /locus_tag="P5313_00730"
     CDS             complement(7549..8490)
                     /locus_tag="P5313_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY78915.1"
                     /translation="MPDINDCAALVENASRVVVGKRRALELMTIALLAEGHVLLEDVP
                     GLGKTIMAKALAASMGGVFRRIQFTPDLLPSDVTGFSVYDQKSGDFVFHPGPVMANVL
                     LADEINRTIPRTQASLLESMSEFQATVDGSTRVLPRPFFVIATQNPIEMDGTFPLPEA
                     QLDRFLMKIDLGYPTMEEELDILERFRKADPIPELRAVTTPERVRELQAFRGDIRVAP
                     PIRRYIAELAAATRAHPRVRYGASPRGSLGLLKAAQASALLNGRDYAVPDDVKILALP
                     VLAHRLILRHEERAKGASAAAVMAEILAGVSAPSPAG"
     gene            complement(8573..9805)
                     /locus_tag="P5313_00735"
     CDS             complement(8573..9805)
                     /locus_tag="P5313_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY78916.1"
                     /translation="MTSSPDTTFSIGGVAFKNPFLVGSGPTVTTVDQIRRAEDSGWAG
                     ASIKLAVEPFPYLNFPPRYRWLKKSRYHIFTAERRLTADESLRLTEEACRKTSSDFVV
                     IPTMTYDGTDLEGWGRLARRFVDAGARIVELNLCCPNMSFNLSSTGASTTKHTGASLG
                     NDMEEMPKVVRVVSEAAGVPVIAKFSADGNLAPLAGRTAVRAGAAAIGHNGNLLGIPD
                     IDIRDPLKGIYRLQDQITLGCMSGPAIRPLALRTTYQMRQAAGPDAVLISSGGATDLA
                     SSVEHLLVGADAVWICTQTMLRGFDWLPRMLDQFTAYMRDMGFVRLADLRGLLSRNLA
                     SAGELTVHEGRAVLDEGKCSACGACWNIGHCCAISGAGGRTAYDPTLCRACSTCVDLC
                     PTGAFRVVRSDGAEEPVT"
     gene            10215..11216
                     /locus_tag="P5313_00740"
     CDS             10215..11216
                     /locus_tag="P5313_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78917.1"
                     /translation="MIPSTTPSEPPPASARLQLREASDLTPGYGPVTAQAWRRDGATL
                     LTADFDRIQRWDAPGARNLGAIAGTGGLIIDFSLFEPASGKARLAVAARDGTTTVRDP
                     DDGSPLASVSVDADCAAWSPDGSALAVCLTDGRVLVLAWPGLKTSAELPAVPGRAPLR
                     AAWFPSGRELVLGGASGGLARWRPGEPAARPLGPRPSAGSAEADAAGTDAGKPAVLAL
                     AVSPDGSRVAAAGSDGTVTVLDLVSGAAVLRFQAHRGAALSVAWSPDGTRLATGGQDR
                     QVRVWAAADGSPYAMQHHNSLPVRSVAWRPGGSFLSSGAGSMDLKAHTGATILWEVP"
     gene            complement(11308..12009)
                     /gene="radC"
                     /locus_tag="P5313_00745"
     CDS             complement(11308..12009)
                     /gene="radC"
                     /locus_tag="P5313_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRY78918.1"
                     /translation="MAESSSYEVRPEAYGVKPDIRERLLQSSPGHISDADLLAVILGT
                     GSRGRDVRTLAREILARIDGERRTPAAEDLARLPGVGPAQACRVTAALELGRRLYGFR
                     ERRISGPRDVFPLVSHWADRTKERFAVLTLNGAHEVLAARVVSVGLVNRTVVHPREVF
                     AEAISDRACAIIAAHNHPSGRLDPSPEDREITERLRDAGATLGIPLLDHVIFCPDGYW
                     SFVEHGLLEPPEEGG"
     gene            complement(12106..13752)
                     /locus_tag="P5313_00750"
     CDS             complement(12106..13752)
                     /locus_tag="P5313_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY78919.1"
                     /translation="MRLRSVEPGVKGYPLLLGILAASASLLWGVAVAGSLSEHRAAVL
                     NAEDSLTRMTEVVLEQTGGLFGEVRVYLKLLDAWLQDNPEADPRTDPRFIRLVDSLRR
                     EGRIRIDLRLVSRDDGLFYIPSPDTSKALAQVGDREYVRAQKDEATRGFYIAGPVLSR
                     VTGIWGIPVSYPLTRGNAGMAILFAAIELPTLNELYESARPKPDGSITLVRQDGIILA
                     RAPFDAKEVGRNLAAERVWRDRREGFDRKVVAIDNVEKFIVLRALPDLPLEISVAQPV
                     ASALGPWRRETSLRFAILAIATAVMLVLYRRMLGNWRALTLSESNVRILNRELGDTVE
                     EVRRQLAEKEAIIRETNHRVKNHLAQVVSLVRMTRMEESTPKMEALEARLEGYRLLYE
                     KLSYKPGEGERLDAGDYLEDLLSRLVEVTPSDRPVTAAFHSEPLAVTTKTCSALGLML
                     GELVTNSIKHAGPGEGGLRIECSVRADREGLRMTYSDNGPGFDYPSVDRSSIENHIGL
                     ILVESLVKQYEGTLTYRRDPGSRFEILLKTGEPSPDAPAA"
     gene            14009..14443
                     /locus_tag="P5313_00755"
     CDS             14009..14443
                     /locus_tag="P5313_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY78920.1"
                     /translation="MQGTRILDYIYAAHRYLAELESVPREYGSGEKLFASEIHTVAAV
                     FHSPGCNLTSLAAALDVSPAAASKFVAKLIRRGYIVKYKAPDNKRDVLFEATEKGRAA
                     AKGHERFEKRTFANLIRAEETLDPREREAVRGYFEKLFALVK"
     gene            14979..15776
                     /locus_tag="P5313_00760"
     CDS             14979..15776
                     /locus_tag="P5313_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY78921.1"
                     /translation="MKAVSGGRPGRRGFRKALLLGSALLFPVTMLYFSPGMVFRGARL
                     GILSGSYLTFAALFLGALVAGRAWCGFLCPGGGLCETARAVNDDPFRRGGLKAVKYVV
                     WILWLAGIALVAVFVGHGFHAVDPLLGTDRGVSIHSLELWPFYYLVIGIILALSLSLG
                     RRGFCHTLCWMAPFMVLGRGLRNAMRTPALQLAADPEGCPGCGACRKVCPMSLPVPEL
                     VRTGSLEHRDCILCGECVRACTKGCIRLEFGRPSAVGVRSGMNRGER"
     gene            15839..16507
                     /locus_tag="P5313_00765"
     CDS             15839..16507
                     /locus_tag="P5313_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78922.1"
                     /translation="MYFLGPVLWHAWMSFRLGFLALEESCRCLLSPWAIAIAVLLFTL
                     NAASLAKGVERNGEGEGERAAAGRVAVHFASLVAFATIGTAASMCALRGADAGGLPPL
                     LKPVVGSLNGASMCFVFYASSSANLIARISPPGRGSSEGRRRILSMSRACNALLFAIG
                     LPLFLATSLSAARLGGGIPADRGMNRLLASLVVPATMGSALFIRADRRLAAAPGGDGK
                     EIAR"
     gene            16696..19722
                     /locus_tag="P5313_00770"
     CDS             16696..19722
                     /locus_tag="P5313_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY78923.1"
                     /translation="MFTRSVRNPKPAGLVACLVWLIAAAGSFGVETPFRFTSIGAREG
                     LPNSSVAAIVQDSRGFLWFGTQNGLVRWDGYTFKLFENAPFVSDTLVHNQIQTLLLSG
                     DTLWIGTYGGLNRFDLRTESFTAYRSDPKDPGSLSADLVISLCEDSAGRLWVGTSRGL
                     DRLTEKGTFVHYRSIPGDPRSLGADLIRSVFEDSRGTLWVGTSGGGLARYQPVSDDFD
                     VVRAGPGSSLPSDYVMSISESPSGELWFAFWYGGIARLADPESLRFEHVRLADDRTYF
                     VDARRGDVVLAGSWGGGLFAYHPATGALERYRAADGPGSIAGDVVYSSLLDEDGAFWI
                     GTNGNGVSRTERSDLRYEAYQHGDDPGSLAAGKASAILEDSRGRLWVGIYSGGLDRRD
                     PGKTSFAHYRRDPRNPRSLPDDIVNALFEDSRGEIWVATNGGLCRYVERTDDFQVYRN
                     DPKDPDSFADLVAYSLAEAPNGDLWVGTYTKGLDRYERATGRFIHYPPDETDPSKPGA
                     ALVYALAQGRDGTLWLGVGNGLERYSGGVFTRYRYDPAKPDGLAALSVRSLLVDSRGA
                     LWLGTVGGGLQRLVDPEAGTFLRYGRAQGLPDNSVRSILEADDGSIWVGTPFGLAIVD
                     PDRGAIRGFSVFNDLKDRDFHVGAWKSRDGSLYFGGQGAVYRIHPSSETSARKPPRLL
                     VSELRIGGSPSRDLTAPSYLDSVELPYDGNSFTISFAASDFRDPRRNRFSYYLEGFDK
                     AWSPVSPARSATYTNLRGGTYVFRVKASDNEGVWNEEALALRVRVLSPPWLSPPAYAL
                     YLGLLAGLWYLTASTLRGRELRARIRELTELKGRLEEANDRLSDLSLQDPLTLVANRR
                     RLDAELQRSHASAVRDRRPLSLLMVDLDHFKDFNDRYGHLAGDEALKSVAELLRKEVS
                     RASDFVARYGGEEFSVLLPNTAEEGASRVAERIRSQVEALGIPNEALGSAAVVTLSVG
                     ISCVVPGPESTPEAIVAAADAALYRAKAAGRNRVSF"
     gene            19865..20440
                     /locus_tag="P5313_00775"
     CDS             19865..20440
                     /locus_tag="P5313_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRY78924.1"
                     /translation="MKSVKGTETEKNLLTAFAGESQARNRYTYYASKAREEGFVQISQ
                     IFEETANQEKEHAKRLYKFLEGGEAMVSASFPAGTIAGTSVNLMHAAEGENYEWKKMY
                     PGFAKIARKEGFDAIATVFEAIAVAEKQHAKRYEKLKANVDAAQVFKKEKSVTWRCIN
                     CGYILDAVEAPKACPACAHPQAYFEVLAKNY"
     gene            20479..20853
                     /locus_tag="P5313_00780"
     CDS             20479..20853
                     /locus_tag="P5313_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HRY78925.1"
                     /translation="MAKPMEVYKCMKCGNIVEVLHGGDGDLVCCGEPMKLLKENTSDG
                     AKEKHVPVVEKTAEGYKVKVGSVAHPMLPEHYIEWIELVADGISYRAFLKPGSAPEAF
                     FPVKAKSVYAREYCNLHGLWKA"
     gene            21005..21163
                     /locus_tag="P5313_00785"
     CDS             21005..21163
                     /locus_tag="P5313_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRY78926.1"
                     /translation="MQKYVCDVCGYIYDPAVGDPDGNVKPGTPFEKLPADWVCPMCGA
                     DKGSFSPA"
     gene            21304..21594
                     /locus_tag="P5313_00790"
     CDS             21304..21594
                     /locus_tag="P5313_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78927.1"
                     /translation="MISREDFIFAIGFDGAEAVVDGRAKREFGKLSIRELTEAGQWRA
                     AFAAALWSGKDEDIQEFRSLYNRASGSDYKTVDELKRLFGVQKEEIAKALVL"
     gene            complement(21665..22900)
                     /locus_tag="P5313_00795"
     CDS             complement(21665..22900)
                     /locus_tag="P5313_00795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRY78928.1"
                     /translation="MDAKVDIRLKPREEDRILAGHPWIYDNEIARADGPAASGAEARV
                     FAARGRFLGSGTLSPGSKIRVRLHSRSDEDWDGAFTRRLLETALAFRRRVRDLSSESA
                     RIVFGEADGLPGLVADRFVGTPLGPDGEPAGEPGSWIVLQVLAAGAEARKAGIVEALR
                     GALGPDGILERSEAPVRRLEGLSPSVGVLAGSVPDRIDIRENGLSFVVDLAGGQKTGW
                     FLDQRENRAAAAAYAPGRTVLDAFCNAGGFGLCAARAGAASVLSVDSSEAAVAQVREN
                     ARRNGLENKVEVRAANAFDLLRDLEKEGRRFGLVVLDPPAFAKNRAALEGAARGYKEI
                     NLRALRLLEPGGVLATCSCSWWFDRERFRAMLEDAAADSGRRLRYLEDRGQAPDHPVV
                     SGYPESWYLKCVIAEIVNR"
     gene            23151..23891
                     /locus_tag="P5313_00800"
     CDS             23151..23891
                     /locus_tag="P5313_00800"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRY78929.1"
                     /translation="MKRPVLCAAAVLAVLLPLSSCGTRVSASPLGLAGNGDVVARELR
                     VSGFDAVAFGGAGILRFHRAPAHRVVVTLDSNLLESFQAELRGSTLELGFKPGFQINR
                     YSKLEIDLYLPDLKGITLSGSAEAALLDSFRGSTFQANLSGSGRLIGEVRMDRIRVTM
                     SGSGNVELGGEADRLEADISGSGVIKAARLEVRSARASLTGLGFLDVRVRDDLDIRLS
                     GAGRVRYFGDPKVRSSVTGAGTVERAGA"
     gene            24032..25885
                     /locus_tag="P5313_00805"
     CDS             24032..25885
                     /locus_tag="P5313_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY78930.1"
                     /translation="MAQSKSGRWILSGIVSLALGAAALTTLASCSAAPAQRPETVAAA
                     PEPETKPSDDYSPKPPVPPPAPGPSGDKGSAAAAASESLKMEEFSKDRAAGAPASPSA
                     PGRPSAPPSASGLKAGFSDDNAQFNYFLGFLEEFGHVPNHALEIGERITLRIQDSAGK
                     AVPNARVAVRAGTRVLDQGLSYADGTFRLYPLIYETTAASLSVRVESQSGSASLDVTR
                     DGPRRVVVKLDRPRVLPAPLPLDVLFVLDTTGSMGEEIERLRDTIEIVHANIRAMTPK
                     PAVRFGMVLYKDRGDEYVTQVVPFTADLPAFQAALAEVYASGGGDTPEDLQAALEDSL
                     KKMDWNKDGVRTAFVITDAEPHLDYGQSYTYAHAAKDARARGIRFHTIGTGGLAVEGE
                     YVLRQLAQFTEGKYIFLTYGETGESEGGAPGSVSHHTGSNFQTDKLEAIVIRFVKEDL
                     ANLSDVPLSMDEDYFEATKIPEEERDATLDKLFEEALRNLTDYSTFKVEKDTPAAVLP
                     LVPNTPDLASNAEYFGERLLLTAVKSKAFKLVERKSLQSVLEELELQLSGLADEKAAA
                     RAGELLGADILVTGSVFRRDGKFEIFLRLVRVSTAEVLSVARARVDAKLGL"
     gene            26210..27628
                     /gene="radA"
                     /locus_tag="P5313_00810"
     CDS             26210..27628
                     /gene="radA"
                     /locus_tag="P5313_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRY78931.1"
                     /translation="MSAQKKKPTVFRCSSCGHEEAKWLGRCPSCGEWNTLREAAASAS
                     SVRAAGAAGTAERWSLPLAAVDPAEGSRFPSGMAEVDRVLGGGVMKGSSVLVGGEPGI
                     GKSTLLLQVAAKAGVSGRVLYVSGEESAPQIRLRADRLGLAREGLEILCSGDLELVLD
                     ALEKLKPRAVVIDSLQTLRSAEAGSVPGTVNQVKYCALALSEWAKEAGSAVFLVAHVT
                     KEGAIAGPKAAEHMVDAVLYFEQTDGELRFLRATKNRFGSTDEVGLFTMTETGLAEVA
                     DPSRLFLTRREGGLPPGAAVAPLWEGSRCLLVEIQALTVPAKASLTRVYSDRVDAGRV
                     ARIAAVLERHGGLRFSDQDIYVNVAGGMRIAETGADLAVAAALYSARSGLSLPDRCAV
                     AGELSLAGEVRPLRRMRARVRACGALGYAEVLGPVPDDGEREPGWTGAATIKEAIRRL
                     FGPGARGASGGKPGPEGRGESV"
     gene            27625..29628
                     /locus_tag="P5313_00815"
     CDS             27625..29628
                     /locus_tag="P5313_00815"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRY78932.1"
                     /translation="MKLSIETRKSGVVVPLGALRTARSGGVGEYPDLPVLGELCERCG
                     LSFVQLLPVNDTGFQSSPYSALSAFALHPLYLRLADLPEAAAFRAEAESLASRFGGRK
                     RFPYPEILSAKLALLRRIFDTALPAIRKDPALAAWIGANPWVRDYAVFLGLKEEHGGK
                     SWKDWPARRDPGAGDIEALWEEPGRRDGHLFHAWVQLRCEEQFLAASRALEKRGVALK
                     GDLPILMNDDSADVWAHRSWFRTDRIAGAPPDMFSELGQNWGFPIYDWGALAREGYSF
                     WTGRLAQAAKFYGAYRIDHVLGFFRIWALPEREESGYLGAFEPGAAVSRAELAAAGFS
                     DGRIRWISEPHIREAALSEAASRGAAAAVAAEGIAARAAEALLDRIGSEPLFLFKRSV
                     RGEKDIRAAGLPEALRDFLQAAWRDRVLIPLGPDSFAFAWRHPSASAWPSLSDSERSA
                     LEGLLRAKAAESEREWEEHGRRLLSVLKDAADMLPCAEDLGAVPPLVPKVLAELGILG
                     LRIPRWTRRWDEPGQPYVEPGDFPRLTVSAPSVHDTSTLRDWWEHEAGPADRTAAYGA
                     APPESWLPEVGARVLARFAAGASVLLVVQLQDLLDLSPAYASGDSRADRINVPGTVAD
                     SNWTWRMPVTLEDLAADPQWAGRVGRSVDRSAARRAASPAAGP"
     gene            complement(29522..30448)
                     /locus_tag="P5313_00820"
     CDS             complement(29522..30448)
                     /locus_tag="P5313_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRY78933.1"
                     /translation="MKNKLTRELAVCGLSAVRALLDRNPDRVRRFFLAESLLPEFRTV
                     LRRFAERKTLYRMVSPEELERLAGTVHHQGAVAMIEAPEVPDLDAATAAGWAARGERI
                     LVLDRLGNDHNLGAIVRSAAFFGVRNVVLAGPSAAALVTTSAFRVAEGGMEWVNLYGA
                     SDAGELARRAGPSLAILGADHRGSRTVREALSALPTGRAVALVLGNEETGISPASRAA
                     CSALVRIPGTGDLESLNVAQSAAVLLYELAAGAPAGQAGTGPGRPSGPSAPEPAVPRA
                     PRPERPPASPRTGRPSAPPSRPTGGRRQDPPE"
     gene            30540..30914
                     /locus_tag="P5313_00825"
     CDS             30540..30914
                     /locus_tag="P5313_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY78934.1"
                     /translation="MTDVQVSAVNEETIDTILAGDVEFSGTMVFKKPLMIKGRVSGTI
                     RSESDLYIDTGAVVEAEISANQVSVRGTVKGNVVAKDRVDLYASCSLDGDIRAPEVTM
                     ETGCRFNGICTMTGKKADAPAS"
     gene            30898..31545
                     /locus_tag="P5313_00830"
     CDS             30898..31545
                     /locus_tag="P5313_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78935.1"
                     /translation="MRRLPDPRIAFLALILALATGPASDLGAQTPRPDALKLYREGRY
                     EESRVQCLQEISQDPANIEAYVVLGWSLVALGRHADAEVYARKAYDEVRKDPRVMETL
                     GEAAYFLGKNDQALRWFQSYITALPEGSRVASSYQYMGEIYIRTSRWGHADIALRTAL
                     QFEPSNARWWARLGYAREMGGDLRYSLEAYDAALKINPGLTDAQLGRDRVSKRLR"
     gene            31547..32962
                     /gene="mtaB"
                     /locus_tag="P5313_00835"
     CDS             31547..32962
                     /gene="mtaB"
                     /locus_tag="P5313_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRY78936.1"
                     /translation="MPFRAAFRTFGCKLNQLETEALAGAFRESGTVLVPFSESADVYV
                     VNTCTVTGKAEQKARREIRKALRSNPRAVVLVTGCWAEVGTEEVAALGPRTVVIPGSR
                     KDDAALLASDLAEAWEKGMDLAEAAERGRDARAGRAHDPFAFRPETFLLHSRASLKIQ
                     DGCDNRCAYCRVCLARGPSRSLEPGGVVERARALAVRGAREIVLTGVNLSQYRAGGLS
                     MAGLLTLLVRETEGVSYRLSSWEPDKVDEEFLTAFALPRVRNHLHLAVQSGCDAVLAR
                     MGRGYRSETVRRAVRDLRAAKADPFLGADLIAGFPGETEAEFQATFDLSAELTFAWIH
                     AFPFSPRPGTKAFGMRPRVPERTAGARVEALLDLGRRRRAAYAARAVGRLTRAVLEDP
                     AEEDGAEASGVESEYEPGPDPAREAAVAAISDEYLRLEVTGIPESLRRRGSEVSCRIV
                     EALPGAGDLGIPDARAEFVGP"
     gene            33038..33799
                     /locus_tag="P5313_00840"
     CDS             33038..33799
                     /locus_tag="P5313_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78937.1"
                     /translation="MRRMLPVLILAPLLLLGGCDAFFTNVFEGLYTLEVPSAAELGTL
                     PVSDLERLAEDDQFFEELAGDPAKEDAVLENLDNYFGPEGDGTVDTPEEQRAAALYAE
                     VLLRTSGADRVVAGLAGYLASADGFDQLETNPGPELAELFGESLRTLAEIEAMVAALD
                     AAWDAYAAIGAGLATSPLDESLNAGDIAFGAALAAALKGVTVTSGQTDAEYIYASIQG
                     TAGGMSADFSTTVLSDTEMDYLFLAAGFDPADLGL"
     gene            33813..34958
                     /locus_tag="P5313_00845"
     CDS             33813..34958
                     /locus_tag="P5313_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78938.1"
                     /translation="MKRIGIVLAAAVLLLAPLTAQDIPLSGFSPKDARALGMGGAFTA
                     VSEGFASLYGNPAGFAASEGELTILAPMSWLYLRPTSDNLALLRSAVADDGAAVDALE
                     TLLTDNGLGGGASLGIGYVGKGLGIGAFVTADAYAAGDTLAGAVLDVDLQFNAVLGLA
                     RTVKVFGLGLTLGGNLRPFYRAVGDIPLSEVVGAMMGTADPGDVFLSKELISGFGLAA
                     DLGASLELGAFKLGLAVRDIAPAFQAYRNTVRDLADSGFVTSSGTPAGSFALTPNVVL
                     GASWHPDLGAFSRILNPRVLLEIQDPLAAIRDRASFWNLVHAGVEVKTLGFLALRAGV
                     NKGWLSAGLGLDLFFLEIDAAAFTEELGWRPGQNGRSGVALQAAIRL"
     gene            35102..35785
                     /locus_tag="P5313_00850"
     CDS             35102..35785
                     /locus_tag="P5313_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78939.1"
                     /translation="MKRTRYIAIVAAVLAALTLASCQDFFTSSFASWAQRDPSVPSGL
                     TADQAMSIADQAVANRDTALAQALLPQMAGLIATGTPPAALVASAVDTAVLASGIDDA
                     FGTILTDIGIEALTDDPTVYAAQIDTIISSIAVDVDSQAIFTALAAADPNVMAAAGAT
                     ASDYATAAVALVLAEAAANSIDFFSDSPDITTFTCYSTVEMLMDNASATWPNDSIVDV
                     LADFISYVP"
     gene            35841..36956
                     /locus_tag="P5313_00855"
     CDS             35841..36956
                     /locus_tag="P5313_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78940.1"
                     /translation="MTYLLLILSLPLSAQTAAPGVPVSPRRAAIGGLHAADLAGFDSI
                     FTNPAGLASAEGEWMYSRIALNAAGPIFDIANMALGGTDILSNLTSLLDPAGRLYTSM
                     DLATPVALGYVGKGLGIGIFNRTYVIVNAPSVTTSFLTFGEELLLTGGYAKRFDLGEG
                     NLLDLGIVVKGFLRNEFAWSGALTDVSTAFNDIFGGIPFNIVTGMGVDLGATWTFGDY
                     ALALTARDAFTPYIASSYTSYNGFKANPTANKIETVTDLMPIDLSLGFRWNPRWLFLA
                     RTGSELTLLADYRNIWGLFRILDRNPILELSLGTEIVMLEILSLRAGIRDTLPSAGFG
                     LDLQVFDLSLAMYGKELGLEPGQRPVFNLDFAIDFRY"
     gene            37063..37135
                     /locus_tag="P5313_00860"
     tRNA            37063..37135
                     /locus_tag="P5313_00860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37096..37098,aa:Lys,seq:ttt)
     gene            complement(37497..38327)
                     /locus_tag="P5313_00865"
     CDS             complement(37497..38327)
                     /locus_tag="P5313_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY78941.1"
                     /translation="MRSFVGLTEFRGLLDSLTAKGAGEFLVSPGDIDPPDQVYGAIQE
                     KLGSSFPWFPMIGNHEAETPEDMDYLRAFPVETQYSVENFLGGPPGSEETCYSFDFGG
                     AHLVFLNQYYDGFTDIGLDPPPSDPFAGTISPSLLEWLRTDLESANLRAPAFILVFGH
                     EPLWPMADEATGRLRHDGTSLDAHPVEVRSFIALLQEYSVTAYVCGHTHNFSAALVDG
                     VPQIDAGHGRGDADAGAPSTYLLFDLYENMVKISAFRDPGIPDGNYELAREIRLLSRP
                     "
     gene            complement(38554..39054)
                     /locus_tag="P5313_00870"
     CDS             complement(38554..39054)
                     /locus_tag="P5313_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78942.1"
                     /translation="MKLCRRARLTVIAVMAMTIAPLSAQAPTPRVLFVYDEVNDQTKP
                     YIAHFREAFTSSGIPFDEAAAVDLESKDLSRYEVLVLHGIVQAFNSKSPIRDWLKTKP
                     PIAGKRVYLFVTANRWFLDNLFKDLNGLLEKNGAKPVDAVSMATKETDDSLEKTAVKS
                     FVGKVR"
     gene            complement(39219..40007)
                     /locus_tag="P5313_00875"
     CDS             complement(39219..40007)
                     /locus_tag="P5313_00875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRY78943.1"
                     /translation="MKSPSRITAVSILTIALLLLAVSPLAAQAKKPVQTNASFTAADG
                     TKLGAYLALPPGFGKHPGVIMVHEWWGLSGDIAAMADRLAKEGFAVLAVDAYRGKLAQ
                     TAAEAGALRNGTPPTQIAADLDAAYEWLTRHPRVDGSRMGTLGFCFGGAQAMLLGTRQ
                     AGLGAVVTLYGTGLIQQESALGELGRKGAVLGIFGDTDAGIPPAQRQGFKDALAARKA
                     RYAESVYTGVGHAFVKDENVDVPGPARQAWRQTIAFLQDELGVK"
     gene            complement(40148..>41236)
                     /locus_tag="P5313_00880"
     CDS             complement(40148..>41236)
                     /locus_tag="P5313_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY78944.1"
                     /translation="KAGGSDYLVKPVTSESLEHLFIREYEKSTIEHENDELDPFLSPE
                     SSEVPFIHADTRMREVLSAVYRARESSATVLITGESGTGKELVARLIHDSSDRRDKPF
                     VTVNIAALPETLMESELFGHVKGAFTGADQSREGRFAEGDGGSLFIDEVGDIPLGIQV
                     KLLRALQFGQIQRVGENATRTLDVRIIAATNRNLKGMMELGTFRADLYWRLNVIPIAI
                     PPLRERREDIAALTRWFIEKFSKEYGRPVRGISREALSALMRHPFPGNVRELENAVER
                     AVLMTRREILSTQDIQLESSSGATEPEGAASGTYDEVVGRFELDLIAKALEAAGGNRS
                     EAARALGIGERRLRSRLKVLNEKLGPGR"
BASE COUNT         6468 a        13804 c        14165 g         6799 t
ORIGIN      
        1 ctgcaaccac gccgttcacc gtccaccgac cactgtccac cgttccggga gccgcgatca
       61 tgattgagac cttaacgccg ggatccggcg tacaacgatc ccttgtacgt gcggcgaccc
      121 ttctagctgg cgtctacgtt gccgcccaga tgcttgcgga catcgccagc ctcaagatcg
      181 ccgtggtggg cggattggcc gtggacgccg gcacgttcat ctatcctata accttcacgg
      241 tccgggacat gatccacaag cgcctcggga agtccgcggc ccgaaccgtc atcgtcctgg
      301 ccgccgggat caacctggcc atggccggat ttttatggct ggtcagcgtc ctgcccgcgg
      361 acgcggcctg gtccgcctgg gaaggggcga acgtcaccgt gaacgacgcc ttcgcacggg
      421 tcctggcccc ggcctggacg atcgtcctgg ccagtatagc cgcggaggtt gtcagcgaac
      481 tgatcgatac cgaggtctac cacctgtgga catcccgggt cacctcgcgc ttccagtgga
      541 cccgggtcct ggtgtccaac gcggccagcg tgcccgtgga cagcctgatc ttttcctggc
      601 ttgccttcgg ctggggcttc ggaatgcccg cgccacaagt ttggcagatc ttctggttca
      661 acgtggccgt aaagggtgcg gtgaccctgg cttccctgcc gggcatctac ctggtgcgcg
      721 aacggggtgt ccaataagtc ggttacttat tggacacccc gttcggcaac gaagccctcg
      781 ggcttcgtcg acatttcgca gaatgccggg aggcggggac ggagggcaac cttgacgcct
      841 cgccccgccg aagcgtacaa ttcttaccta atttgaccgc aaccgggact accgccttcg
      901 gcggacagtc cggccggacc cgcgcgggat acccagcgtc gggaacccgg ctccagacgg
      961 acggcggttc cgaagctccg gaaccccgtc cacaacccgg catcgcccgc cgcgctacgc
     1021 ggatcacggc gagccagtca ccgcgcgcgg aagcgtactg cgaggcggga aggagcgatc
     1081 ttggacattc tcaagaaatg ccgcgagttc gacgagcaca agaaggtgat ggccctcggc
     1141 ctgtacccgt tcttccagcc cctccagggc acggagggga ccgaggtgac catgtccggc
     1201 cgcaaggtca tcatgctcgg ctcgaacaac tacctggggc tcaccttcca tccgaaggtc
     1261 aaggccgcgg cccagaaggc catcgacacc ttcggcacca gctgcaccgg cagccggctg
     1321 atcaacggca ccctctgtat ccacgaggag ctggaaaagc gcctggccgc ctacatgaag
     1381 cgcgaggccg ccctggtctt ctccacgggg atgcagacca acctgggcac catctcctgc
     1441 ctgatcggcc gcaacgacat cgtcatcacc gacaaggacg accatgcctc catcgtggac
     1501 gcctgccgcc tgggctacgg ggagatcaag cgcttcgtcc acggcgacct ggaccagctt
     1561 gagcgcatcc tccagaagat tcccgaggag aacggagtcc tggtcgtggt ggacggggtc
     1621 ttctccatgg gcggcgacat cgtggacctg ccccggctgg gacccctctg caagaaatac
     1681 ggcgcccgcc tatacgtgga cgacgcccac tccatcggag tcctgggcgg gggcagggga
     1741 accgcggccc acttcaacca gtacggcgac gtggacctga ccatgggaac cttctccaag
     1801 agcttcgcgt ccctgggcgg cttcgtcgtg ggcgacgcgg acatcatcga ctacgtgaag
     1861 cacatggccc ggtccttcat cttctccgcc tccctgcccg cccccaacgt gatggccgcc
     1921 atggcggccc tggacatcat ggagaacgag ccggagcacg tggagcacct ctgggagaac
     1981 gcccggtaca tgatgaagag ctaccgggag ctgggcttca acatcggcgc tacccagacc
     2041 ccgatcatcc ccctgatcat cggggacgac atgaagtgct tcgccttctg gaaggaagcc
     2101 tacgagggcg gggtgtacgc caacccggtc atcgcccccg cggtgcccca gggcatggct
     2161 cttcttcgca ccagctacat ggccacccat acccgggacc agctggaccg agccctcggc
     2221 attttcgaga aggccggcaa gaaggtcggg gtcatctagc gaagtcccag gatccccggg
     2281 acttcccggc ggggctgtcc aaaaagctca gatagctttt tggacagctg gtattagtgt
     2341 agcagtttct aacgttctta agagaacgtt agaaactgca aggggtgtcc aataagtaac
     2401 cgacttattg gacaccccgg acttgaccgt acgggtattg tacggctgga tccgccctag
     2461 ttgtgcccga atccaggaag cgccgcccga tcgggcggcg cttcgtttta tcctgttccg
     2521 ggtcaacacc tgccgccgta gacttcggcc tgcatccggc gtttccggtt gcgcccgggc
     2581 ggaatgaggt atactttata gacagagcgg agatattcga ggggaatgcc gatggatcca
     2641 cgtacgatcg ccctgctgat gctcatcagc ggggtggaat gcagcctgct gttcttcgcg
     2701 tcggggatct ttcgaaaaag aaccgatgga ggagccgggc ggccctccct tttctgggga
     2761 gccggcacga cggttcacgt cctgggggtc ctggggctca tgacccaggg gatcgtcggt
     2821 ccctggttca gcgtggtctt cgccaattcc gcgatcatcc tgggccagtt gctcatcctg
     2881 gtcggtatac gccgcctgac ggcaataaac ccggccctcc cccgatacgc gtcggcctgg
     2941 ctgctctatt ccgcggcggc ggtcgtcttc acgttcgtcc tgccttccac caccgtgcgg
     3001 atcatcctgt tctccgcggc catctcggcg ttctatatcg aggccgcgat cctggtcctg
     3061 atttccaagt cctggaaacg ggagcccctg accgtctacc ttaccggaac ctttctggcc
     3121 atggcgggtt ttttcgccgc ccgagccctg gtcacggtgt tcgtctcgcc ggcatcgatc
     3181 ttttccccct ccgccatgaa cctggctacc tacatcgtgt cccacgtcgg tttgattggc
     3241 tggagcttgg ggctcatcct cctccagcag cgacggaccg aggcggacct ggagcgggcg
     3301 ctccgggaga aggaggtgct tttccgggaa ctccagcacc gcatcaagaa cagcatctcc
     3361 gtgatcgcga gcctggtgag cctggaagct tcccgagtgg aggatcccgg ggcgtcggcg
     3421 gttttggagg ggctggaagg acgcatctcc gccatagcct ccctgtacga gcacctcttc
     3481 caatccggcc aaaccgacca cgtcgacctc gacctgtacc tgaaggccgt ggtcgagacg
     3541 ctcttttccg gccaggccgc gcgggagcgc ggagtgcggc tgaacctgga agctcaggag
     3601 ctgcgcatcg acgtgcgaag ggcgatcccc ctgggcatca tcgccaacga gctggcctcg
     3661 gactgcctca agcacgcctt cccggagggt cggtcgggca cggtccgggt atccctccgc
     3721 gcggagggac cctcggcggt cctggaggtc cgggacgacg ggaaaggact tccggaggga
     3781 ttcaccatcg ggtcctcgga aggcctcgga ctggtcctgg tggatatgct ggccaagcag
     3841 gtgggcgggt ccttcacggc ggcgtccgag ggcggttcgg tcttcacggt gaagttcccg
     3901 aaagaatgga agagctgacc ttcctgcgtc cggcctccgg gaggaaccgg tgaagacaga
     3961 aggcaatccc attcgcgcgg tcatggacct cttcgacgac cgcatccgcc gcgaggcggc
     4021 ggaccggttc ggaatggacg gggactccct ggaggagctg gagggctcgg cctacgtgtt
     4081 cgccggcgag cgtcgggacg gcaagggccg tgttcggggg atcctcaaga tcaccccggg
     4141 gcttcgggat ccggggccga tcatgggggc cagcagggcg gagctcgagg ccgaggtgga
     4201 ctacgtgcgg tatctcgccg ccaaccgggt gcccgcggct cggagcctac cctccctcac
     4261 gggggcggac gtcgaggaga tccccataga cggggaccgc tcgttcctcg cgtactgctt
     4321 cgagttcgcc gagggaagca tgtttcccga cgacgatctc accgtcttcc cgcccggggt
     4381 actgcgcgag tggggccgac tgctcggaat gacccatcgg ctggctccgg gtttccgtcc
     4441 ccggccgggg gcccggaggc ccgactggcg gggggacgat cacctccgtt cccggctctt
     4501 cgttccggcg tcccaggagg ccgtacatcg gcgcttcgac gagaccgtct tgctgctgga
     4561 atcccttccc gcggagcctc gggagtacgg catcgtgcac ggggatttcc accacggcaa
     4621 cttcctggcg gagggggaac ggctcaccct catcgatttc gacgcgtgcc gattcacatg
     4681 gttctcaatg gacctggcca cggccctctt caactgtctg cccatgcccc ggtcccggac
     4741 cgccctacgc cgcgattgtg ccatggactg gctgtcccgg atcctcgagg gctaccggga
     4801 ggagcggacg gattcgccgt ccctgctggc ggatctcccg cttttcctgt acgtgaacga
     4861 gctcctcgcc tactcgtacc ggtacaagca ctgggaaccc gaagagctcg aagaccgggc
     4921 ggattaccta tcgtccatcc gggagcgaat cgaggaccgg gcgccggtcg tcgagttcag
     4981 gcccggcgac ctcgagtccc tggccgaacc caggcgttcc atgaggtagc cgcggaccct
     5041 cgttccccgg cgcgccgcgg ccagccgacg atccgcatcc ggatcccggg gcttcccgcc
     5101 gtaaacctcc ctctccatgc ggtccacgac gaattccgcc tcggcggcgt ttcgggggaa
     5161 caccgcggcc aggcgccggg cgtactcccg gggggtctca gcccggttcc gcgccagccc
     5221 gaccagacgc ccgacggata cgaggcgggc gtaggccttc aaggccgggc tcgaggttcg
     5281 gcgggaccgg agggtaccga ggcgagccag ggcccgccgc agggccgccg ggattcccga
     5341 aagccagcgg gccaggaagg acaggtccgg gggacggttt ctccgctccg cgggggaggc
     5401 gaggcgccgg actaggtgga ccaggaccgc gacgacgagg gccgcggccg atagggcggc
     5461 gatccccccc aggacggcga cgaagatgcc ggtgagggtc cccccctcgg gatccccggc
     5521 ctccccggga agaatccccg atcccccctc ggaaaggcgg ggcgccgcgg tctcgagcct
     5581 gtagatccgc cccagtagtc ggcctatgag ccgaagtacg gccggagcgg cctccgcgag
     5641 ggcttcaccc gcacgggcgg cgggctccgt caacaggggc atcaggaggg cgatcccggc
     5701 cgcggcgccc aggaacaggg cggatacggc cagcagggag gccagggccg aacgccggga
     5761 gaaggccccg ggatcgtccc cctccctccg ggccagtccg aggccgagga tggagaaggc
     5821 aaggaagggc aaggctgcgg cggcgggacg gccgagttcc ccgccgacca gggcggcgat
     5881 cgagagtacg accaggaaga ccccgatccc ctcgtcgaag cgggcgacat ggaaggcgtg
     5941 atccgacttc cgccgttcca gccacgagcc ccggatccag tggagcccca ggcaggacag
     6001 ggcggccgcc ggacggaccc agcccgtccc ggccgcaagg gccgaggaga gctcgggaag
     6061 ggacggatac tccgcgaaaa cggagaatcc caagaaggcc gcggagaagg cggccagccg
     6121 ggccagggcg tggaacagga cgggtccgcc cagggatccc aggatccgcc ccgtaccgta
     6181 ggccaggaag aggatcgcgg ccgatgacgc ggggggcgtg ggaagccccg cggccagaca
     6241 ggcggcggag accaggaagt ggaccttcaa gccctccgtc agggccagga agggttcggc
     6301 ggccctcatg actcattgtc ctcgggaggc aagaggtccg taaacctgag ggatatcccg
     6361 gccggggccg cccgatccgc gggcggtgcc cggtgatccg ccggggtcgt gctttccggt
     6421 tctcctccgc ccgccggctc ggcatacaag aaaaggacgg gatcccgcct tcccgcggta
     6481 ccggccaagg cggccccggc ccctcccccg ggggatcggg ccaggacgag gaatcccgtg
     6541 tttccgggca ccgtcccggc aaggagggat cggaagtccc ggcccctctc gatcccgcac
     6601 cgggccagga gctccaggac ggctcccagg tgctccggtc cccggccaag gtccagggcg
     6661 gcggggaagt ccctcacggc ccggtccgtg gccagggcga aggaggctcc cgactcggaa
     6721 agcagggaag ccagggaccc cgcgatctgt agggcccgct cgaagccctc gccgtccccg
     6781 gccctctcga agagctcgcc ctccaccagg aggaagacct tgcggtggga ggtgggctgg
     6841 aagatcttct cctggagcgc ggccagccga gcgctggcct tccagtggat gttccgggcg
     6901 ggccggttcc ccgagtattc ccgggtgccc tcgtaccagg cggggtcctc cacgatcccc
     6961 cgggcggccc gtatcccgaa atactcccgg aaggggagat ccggcagggc gagggggagg
     7021 atccggggaa agaccacgag ttcgtccggg aaagggagct cccgttcccg ggtgaagagt
     7081 cccagggggt ctccggcgct cagtcggacc ggcccgaggc tgtggatccc acgccgggcc
     7141 gcggtgaagg cccaggaacc tcgccggcgc tcgaagggca ggaggctcgt ctccgccgtg
     7201 aggcacgagg cccgggtcgc ctcgatcccc gcgggcttgg gcaactcgag gcgaaggcgc
     7261 agggccaggg gggaacggtt gactatctcg gcgccgagct ccgaggactc ccccgcgaag
     7321 agcctgcggt tacccagttc gaaggaggcg gaaaggcggg cggagccgat ccgggtccag
     7381 gccctcaggg cgagcatcag gatcaggacg accgcggaga gccgcgcaag gggataggcc
     7441 cggcccgccg cggcgacggc ggtgagcagg gccagcagga tccccatgcc ccccgtgccg
     7501 aagaggcttc cgaggtacgg ggtgcggctt tcctcggcca tggcccgatc agcccgccgg
     7561 ggagggggcc gaaacccccg cgaggatctc cgccatgacc gccgcggcgg aagccccctt
     7621 ggcccgctcc tcgtggcgga ggatcagccg gtgggccagg accggcaatg ccaggatctt
     7681 cacgtcgtcc gggaccgcgt agtcccggcc gttcaggagg gcggaggcct gggcggcttt
     7741 caggagcccc agggagcccc gggggctggc cccgtaccgg acccgggggt gggcccgggt
     7801 agccgcggcc agctccgcga tgtagcgccg gatcggcggg gcgacccgga tatcgccccg
     7861 gaaggcctgg agttcccgga cccgttccgg ggtcgtcacg gcccggagct ccgggatggg
     7921 gtccgccttc cggaagcgtt ccaggatgtc cagctcctcc tccatcgtcg ggtagcccag
     7981 gtcgatcttc atgaggaagc ggtccaactg ggcctccggg agggggaagg tcccgtccat
     8041 ctcgatgggg ttctgggtgg cgatcacgaa gaagggccgg ggcaggaccc gggtgctccc
     8101 gtccacggtg gcctggaact cgctcatgct ctccaggagg ctggcctggg tccgggggat
     8161 ggtgcggttg atctcgtccg ccaaaagaac gttggccatg accgggccgg gatggaagac
     8221 gaagtccccg gacttctggt cgtagaccga gaagcccgtc acgtccgagg gcaggaggtc
     8281 cggggtgaac tggatccgac ggaagactcc ccccatgctg gccgccaggg ccttggccat
     8341 gatcgtcttg cccaggcccg gcacgtcctc caggaggacg tgcccttccg ccaggagggc
     8401 gatggtcatg agctccaggg cccgcctctt tccaaccaca acccgggagg cgttttccac
     8461 gagggcggcg cagtcgttga tgtccggcat gggtacatca tcccgaggat ggaagcgggc
     8521 gtcaacggat cggggacggg gggccgagga cgtgagcgcc gcgggagtcc tcctaggtca
     8581 ccggctcctc ggccccgtcc gaccttacca cgcggaaggc cccggtcggg cagaggtcca
     8641 cgcaggtgga acaggcccgg cagagcgttg gatcataggc ggtccttcct cccgcgccgg
     8701 agatagcgca gcagtggccg atgttccagc aggccccgca ggcgctgcac ttcccctcgt
     8761 ccaggacggc ccgaccctcg tgtacggtga gctccccggc cgaggccagg ttccgcgaca
     8821 gaaggccgcg gaggtccgcg agccggacga agcccatgtc ccgcatgtag gccgtgaact
     8881 ggtccagcat cctcggcagc cagtcgaagc cccggagcat ggtctgggtg cagatccaca
     8941 cggcgtccgc ccccaccagg aggtgctcca ccgagctggc caggtccgtc gccccgccgg
     9001 agctgatcag gacggcgtcg ggacccgcgg cctggcgcat ctggtaggtc gtacgcaggg
     9061 ccaggggccg gatcgcgggc ccggacatgc agcccagggt gatctggtcc tgcaggcggt
     9121 agatcccctt cagtgggtcg cggatgtcga tgtccgggat tcccaggagg ttgccgttgt
     9181 gcccgatcgc cgcggccccc gcccgcacgg cggttcgccc ggccaggggg gccaggttcc
     9241 cgtccgcgga gaacttggcg atgaccggaa cccccgccgc ctcgctcacg acccggacca
     9301 ccttgggcat ctcctccatg tcgttcccca ggctggcgcc ggtgtgcttg gtggtggacg
     9361 ctcccgtgga ggagaggttg aaggacatgt ttgggcagca gaggttgagc tccacgatcc
     9421 gagccccggc gtccacgaag cgtcgggcca gccggcccca gccttcgagg tccgtgccgt
     9481 cgtaggtcat ggtcggaatg accacgaagt ccgaggaggt cttccggcag gcctcctcgg
     9541 tcaggcgcag ggactcgtcc gcggtgagcc tccgctcggc ggtgaagata tggtaccggg
     9601 atttttttag ccagcggtac cggggcggaa agttgaggta ggggaagggc tctacggcca
     9661 gcttgatcga ggctcccgcc cagcccgaat cctcggcgcg ccggatctgg tccaccgtcg
     9721 tgaccgtcgg accggagccg acgaggaagg gattcttgaa cgccacgccg ccgatggaga
     9781 aggtcgtgtc cgggcttgat gtcatggggc ctcctggatg cggttcaagc tgaacggacg
     9841 cagttccggg ttccggcgct gcaccgcctt catgcatcgc tcaatcgggt cggaattgca
     9901 agaggggagg ccgcgcatcc ccgtgcggaa ccggagcgcc cggggccggg tccgcaggaa
     9961 tagcaagaag tatcaaggtt ccgggcggat tttccggagt agactttcgc ggacctgaac
    10021 tgcctgcgtc cggcgggggg aatgctatgg aaccagaaaa tccttggacg aagatccgac
    10081 tcccggcgtt cccggggatc tgcgcgatca tcctcgtatc ctgtgtgatc gacggagtcg
    10141 ggaccgggta tgcgagcccg gccgcgcaag gcccggaggc atctgcgccc gggactccga
    10201 cggcggtcgc gcccgtgatc ccttcgacaa ccccctcgga gccgccgccc gcctcggccc
    10261 ggctccagct ccgggaagcc tcggacctga cgcccggcta cgggcccgtg acggcccagg
    10321 cctggaggcg ggacggggcg accctgctga cagccgactt cgaccgcatc cagcgctggg
    10381 atgccccggg tgcccgcaat ctcggggcga tcgccgggac cggcggcctc atcatcgatt
    10441 tttccctctt cgagccggcc tccggaaagg cccgcctcgc cgtcgccgca cgggacggga
    10501 cgacgaccgt ccgggatccc gacgacggct ccccgcttgc ctccgtatcc gtggacgcgg
    10561 actgcgcggc ctggtccccc gacggatccg ccctcgcggt ctgcctgacc gacgggcggg
    10621 tcctcgtcct cgcctggccg gggctgaaga cctccgcgga gcttccggcc gtccccggcc
    10681 gggctcccct acgtgcggcc tggttcccct cgggacggga actcgtcctc gggggcgcct
    10741 cggggggcct ggcgcggtgg agacccgggg agccggccgc ccgccccctc ggcccacggc
    10801 cctcggccgg gtcggccgag gcggacgcgg ccggtaccga cgccgggaag cccgcggtcc
    10861 tcgccctggc ggtctccccg gacggatccc gggtcgcggc cgcgggctcg gacggaaccg
    10921 tgacggtgct ggacctcgtg tccggggcgg cggtcctccg cttccaagcc caccggggcg
    10981 cggcgctctc cgtggcctgg tcgccggacg gcacccggct ggccacgggg ggacaggacc
    11041 ggcaggtccg ggtctgggcc gctgcggacg gttcgcccta cgcgatgcag caccacaact
    11101 ccctgccggt ccggagcgtc gcctggaggc ccggagggag cttcctgtcc tccggggccg
    11161 gctcgatgga cctgaaagcc cacaccggcg ccacgatcct gtgggaggtt ccctgaggag
    11221 ggggaggagg atgggggcct ccctgatccg gatgctgggg gagtctacgg gcaccgggta
    11281 cgggtttccg gatcggggtg tcggcgctta tcccccctcc tccggcggct ccagaagccc
    11341 gtgctccacg aagctccagt aaccgtcggg gcagaagatg acgtggtcca ggagggggat
    11401 gcctagggtg gccccggcgt cccggaggcg ctcggtgatc tcccggtcct ccggggaggg
    11461 gtccaggcgg cccgaggggt ggttgtgggc ggctatgatc gcgcaggccc ggtccgagat
    11521 ggcttcggcg aagacttccc gggggtggac gacggtccgg ttcaccaggc ccacggagac
    11581 gacccgggcg gccaggacct cgtgggctcc gttcagggtc aaaacggcga agcgttcctt
    11641 ggtccgatcc gcccagtggg acaccagggg aaagacgtcc cggggcccgg agatccgccg
    11701 ctcccggaag ccgtagagtc gccttcccag ctccagggcc gcggtcacac ggcaggcctg
    11761 ggcggggccc accccgggaa ggcgggccag atcctccgcg gcgggagtcc tgcgctcccc
    11821 gtcgatccgg gccaggatct cccgggccag ggtccggacg tcccggcccc gggacccggt
    11881 gcccaggatg accgccagga ggtccgcgtc ggatatgtgc ccggggctgg actggagcag
    11941 gcgctcccgg atgtcgggct tgactccgta ggcctcgggc cgaacctcgt agctggagct
    12001 ttccgccatg ccgcctcctt ggggcccccg tccggcagcg gcccgggcgg gggctgatcc
    12061 caagacgcgc gggttcgggg tttttgattg cggaagcgcg ggttattatg cggccggggc
    12121 gtccggcgac ggttcgccgg tcttgaggag gatctcgaag cgggagcccg ggtccctgcg
    12181 gtaggtcagg gtgccctcgt actgttttac caggctctcc acgaggatga ggccgatatg
    12241 gttctcgatg gaggatcggt cgacggacgg gtagtcgaag ccgggaccgt tgtcggagta
    12301 cgtcatccgc agtccttccc ggtcggcccg gaccgaacat tcgatccgca gtcctccctc
    12361 cccgggaccg gcatgcttga tggaattcgt gaccagttcc ccgagcatga gtccgagggc
    12421 ggaacacgtc ttcgtggtga cggccagggg ttccgaatgg aacgctgcgg tgacggggcg
    12481 atcagagggc gtgacctcca cgagccggga caggagatcc tccaggtagt ccccggcgtc
    12541 cagccgctcg ccctccccgg gtttgtagga gagtttttcg tagaggagcc ggtagccttc
    12601 caggcgggct tccagggctt ccattttggg cgtggattct tccatgcggg tcattcgcac
    12661 cagggagacg acctgggcca ggtggttctt cacccggtgg ttggtttccc ggatgatcgc
    12721 ctccttctcg gccagttgac gccggacctc ctcgaccgta tcccccagtt cccggttcag
    12781 gatgcggacg ttggactcgg agagggtaag ggcccgccag ttcccgagca tcctccggta
    12841 gagtaccagc atgaccgcgg tggcgatcgc caggatcgcg aacctcaggg aggtttccct
    12901 gcgccaaggg cccagcgcgg acgcgacggg ctgtgcgacg gaaatctcca ggggaagatc
    12961 gggcagggcg cgcaggacga tgaatttctc cacgttgtcg atggccacga ctttccgatc
    13021 gaaaccctcc cgccggtccc gccagacgcg ttcggccgcc aggttccttc cgacttcctt
    13081 cgcgtcgaag ggagcccggg ccaggatgat cccgtcctgc cggaccaggg tgatcgagcc
    13141 gtccggcttt gggcgggcgc tctcgtacag ttcgttgagc gtgggcagtt cgatagcggc
    13201 gaacaggatc gccattcccg cgttgccccg ggtcaggggg taggagaccg ggatgcccca
    13261 gatgccggtt acccggctca ggacggggcc cgcgatgtag aatccccggg tcgcctcgtc
    13321 cttttgggcc cggacgtact cgcggtctcc cacctgggcc agggccttgg aagtgtccgg
    13381 ggaggggata tagaaaagcc cgtcgtcccg ggagaccaga cggagatcta tccggatccg
    13441 accttccctg cgcagactgt ccaccaggcg gatgaagcgg ggatccgtcc tcgggtccgc
    13501 ctccgggttg tcctgcagcc atgcgtccag cagcttgagg tacacccgga cttccccgaa
    13561 gagcccgccg gtctgctcca ggacgacctc ggtcatccgg gtcaggctgt cctcggcgtt
    13621 caggaccgcg gcgcggtgct cggaaagact tcccgccacg gcgacgcccc acaggaggga
    13681 cgcgctggcc gcgagtatcc cgaggaggag cgggtagccc ttgaccccgg gctcgaccga
    13741 acgaaggcgc actataaacc ctccaccaag gcggtacggc cgtcgggcgc gactttcgga
    13801 gacactccgc aaggaatttg aatgggttat tgagattcat cgtacacgat cccggttgcg
    13861 tgtcaaggtg gaatcgcgcc gctgtcgcag actgcgattg acggcgaatt ttccgggcat
    13921 ccggcggcgc cggggcgggc gccgatccgc cttgacaata ggacggaacc cccttgacat
    13981 tattattaac caggataata ataagcccat gcagggaacc aggatcctgg actacatcta
    14041 cgccgcgcac aggtatcttg cggagctgga gagcgtgccg agggaatacg ggtccgggga
    14101 gaagctcttc gcctcggaga tccataccgt cgccgcggtg ttccacagtc ccgggtgcaa
    14161 cctgacgtcc ctcgcggcgg ccctggacgt gtcgcccgcg gcggcctcga agttcgtcgc
    14221 gaagctcatc cgccgcggat acatcgtcaa gtacaaggcc ccggacaaca agcgggacgt
    14281 cctgttcgag gccacggaaa agggcagggc ggccgcgaag gggcacgagc ggttcgagaa
    14341 gcgtaccttc gcgaatctta tccgggcgga ggaaaccctg gatccccggg agcgggaagc
    14401 cgtccgcggg tacttcgaga agctcttcgc cctggtcaag tgacttggtg gattaaaatt
    14461 aaccaggtta ataaaaaaga gaccgccatc ctggacaagc gtgcgatcgc tcgcagggcg
    14521 gcaaggaagg ggactacgct gctttccttc ctcctgatcc tgtttccgct ctccgctcaa
    14581 tccgggccgc cggcgatctc tccggccgcg gcgggacccg cggtgatccg cgtcaccgtc
    14641 ctatgccgcg agccgggcgt actaagggtc gatctcgtcg acgaatcgac cttcggggat
    14701 cccgggcggc cccttgcgag aagggaggtc cccgtcgggg cgggggggct cgcggagccg
    14761 gtgacggtgt ccttcgaggg cctcgcgccg ggacggtacg cggtgcgcgc cttcctcgac
    14821 atcgacggaa acgggcgcct gaacggggga gtcctcggac cggccgaacc ctgggcccta
    14881 tcgtgggcgg cgggtccgag gcgcggaatt ccccgcttcc aggacatcgc cttttcggcg
    14941 gctggagaag tattcgagat tcatatggag gtgaaacgat gaaggctgtc tcggggggac
    15001 gacccggacg caggggcttt cggaaggccc tgttgctcgg ctcggccctg ctctttcccg
    15061 tgaccatgct ctatttttcc ccgggcatgg tgttccgggg cgcgcggctg ggcatcctgt
    15121 ccggcagtta cctgactttc gcggcgcttt ttctgggcgc cctggtcgcc ggacgggcat
    15181 ggtgcgggtt cctctgcccg ggaggcggct tgtgcgagac ggcccgggcg gtgaacgacg
    15241 atcccttccg ccgggggggc ttgaaggccg tgaagtacgt cgtatggatc ctgtggcttg
    15301 ccgggatcgc cctggtggcg gtcttcgtgg gacacggatt ccatgcagtc gatcccctgc
    15361 tgggtacgga tcgaggggtg tcgatccact ccctcgaact ctggccgttc tactacctcg
    15421 tgatcgggat catcctcgcc ctgtccctct ccctcgggag gaggggcttt tgccataccc
    15481 tctgctggat ggcgccgttc atggtgctgg gccggggtct gcggaacgcg atgaggaccc
    15541 ccgccctgca gctcgccgcg gatcccgaag gatgcccggg atgcggcgcc tgccggaagg
    15601 tctgccccat gagcctcccc gtccccgaac tcgtgcggac gggctccctc gaacaccgcg
    15661 actgcatcct gtgcggagag tgcgtccgtg cctgtacgaa gggatgcatc cggctggaat
    15721 tcggccggcc ctccgcggta ggcgttagat ccgggatgaa tcgaggcgaa cgatgaatgt
    15781 cgggagccgg gacgcccggg cgaagcgtct tgcgcggatc gtgccgacat actgcttcat
    15841 gtacttcctc gggcctgtcc tgtggcatgc atggatgtcg ttcaggctgg gcttcctcgc
    15901 tctcgaggaa tcgtgccgat gcctgctttc gccctgggcg atcgccatcg ccgtcctcct
    15961 ctttaccctc aacgcggcga gcctggcgaa gggggtggaa aggaacggag agggggaggg
    16021 agagagggcg gctgcgggaa gggtggccgt ccacttcgcc tccctcgtag ccttcgcgac
    16081 gatcggcacc gccgcctcga tgtgcgcgct ccgcggcgcc gatgccggag ggcttccgcc
    16141 gctgctcaag ccggtcgtcg gctcgctcaa cggagcctcg atgtgcttcg tgttctacgc
    16201 gtcctcctcc gcgaacttga tcgcgcgtat ctccccgccg ggaagaggat cgagcgaggg
    16261 caggcgcagg atcctctcga tgtcgcgggc gtgcaacgcc ctgcttttcg cgatcgggct
    16321 tcctcttttc ctggcaacca gcctgtccgc cgcccgcctt ggcggcggca tcccggcgga
    16381 ccgcgggatg aaccgactcc tcgcttccct ggtggtgccc gcgacgatgg gcagcgcatt
    16441 gttcatccgc gccgacagga gattggccgc agcgccggga ggcgacggga aggagatcgc
    16501 gcgatgaggg tcgaagcctc gatccggatt ccgtcctgga gctcaaaggt ttccgccgta
    16561 tgggtgtcgc ttccgcggac caggtcctgg tcggattgct ggatccgatg taccggggct
    16621 ccccgtttcc gcgcggaacg gcagccgcct tcctgaaccc tggcaaagat ccgcccgtac
    16681 gcttagacta tacgcatgtt cactcggagt gtccggaacc cgaagccggc cggcctggtc
    16741 gcgtgcctgg tctggttgat cgcggccgcc ggttccttcg gggtggagac ccccttccgg
    16801 ttcacctcca tcggggcccg ggaggggctt ccgaactcct cggtggccgc gatcgtgcag
    16861 gattcccggg gcttcctgtg gttcgggacc cagaacggcc tggtccgctg ggacggctac
    16921 accttcaagc tcttcgagaa cgcccccttc gtctccgaca ccctggtgca caaccagatc
    16981 cagaccctcc tcctctcggg agacaccctc tggatcggaa cctacggggg cttgaaccgc
    17041 ttcgacctcc ggaccgagtc cttcaccgcc taccggagcg atcccaagga tccgggatcc
    17101 ctgagcgcgg acctggtgat ctccctgtgc gaggattccg cggggaggct ctgggtcggg
    17161 acctcccggg gactggaccg gctgaccgag aaggggacct tcgtccatta ccggtccatc
    17221 cccggggatc cccggagcct gggagccgac ctcatccggt cggtgttcga ggacagccgg
    17281 ggtacgcttt gggtcggcac ctccggggga ggcctggccc ggtaccagcc ggtgagcgac
    17341 gatttcgacg tggtgcgcgc cgggccggga tcctccctcc cgagcgacta cgtgatgtcc
    17401 atctccgagt cgccctccgg ggaactctgg ttcgccttct ggtacggcgg catcgcccgg
    17461 ctcgccgatc ccgagtccct gcgcttcgaa cacgtgcggc ttgcggatga ccggacctat
    17521 ttcgtggacg cccggcgcgg ggatgtcgtg ctggcggggt cctggggcgg ggggctcttc
    17581 gcctaccatc ccgccacggg cgccctggag cggtaccggg cggcggacgg tcccggctcg
    17641 atcgccggcg acgtagtgta cagctccctg ctggacgagg acggggcgtt ctggatcggc
    17701 acgaacggca acggggtgtc ccggaccgag cgctcggacc tgcggtacga ggcctaccag
    17761 cacggcgacg atccgggatc cctcgcggcg ggtaaggcct ccgccatcct ggaggattcc
    17821 aggggtcggc tctgggtggg catctacagc gggggcctcg accggcggga ccccgggaag
    17881 acctccttcg cccactaccg ccgggatccc cggaatccca ggtccctgcc cgacgacatc
    17941 gtgaacgccc tgttcgagga ttcccggggc gagatctggg tcgccaccaa cggggggctc
    18001 tgcaggtacg tcgaacggac cgacgacttc caggtgtacc ggaacgaccc gaaggacccc
    18061 gattccttcg cggacctggt ggcctactcc cttgcggagg cgccgaacgg cgacctctgg
    18121 gtgggaacct acacgaaggg gctcgaccgc tacgagcgtg ccacgggccg gttcatccac
    18181 taccctcccg acgagacgga tccctcgaaa cccggcgcag ccctggtcta cgccctggcg
    18241 cagggccggg acggaaccct atggctgggg gtgggcaacg ggttggagcg ctattccggg
    18301 ggggtcttca cccgctaccg ctacgatccg gccaagcccg acgggctggc cgccctgagc
    18361 gtgcgcagcc tcctggtgga ttcccgggga gccctctggc tgggcaccgt ggggggcggc
    18421 ctgcagaggc tggtggaccc ggaggccggc accttcctcc ggtacggcag ggcccaggga
    18481 cttccggaca actccgttcg gtccatcctg gaggccgacg acggatcgat ctgggtgggc
    18541 acccccttcg gcctggccat cgtggacccg gaccggggcg ccatccgggg cttctcggtc
    18601 ttcaatgacc tcaaggaccg ggacttccac gtgggggcct ggaaatcccg ggacggttcc
    18661 ctctacttcg gcggccaggg ggcggtgtac cggatccatc cttcctcgga gacctcggca
    18721 cggaaaccgc cgcgccttct ggtttccgag ctccgcatcg ggggcagccc ttcccgggac
    18781 ctcacggccc cgtcctacct ggactccgtc gagcttccct acgacggcaa ctccttcacc
    18841 atctccttcg cggcgtcgga cttccgggat ccccggcgca accggttctc gtactatctg
    18901 gagggattcg acaaggcctg gagtcccgtg tcgccggcgc gttcggcgac ctacacgaac
    18961 ctgcgggggg gcacctacgt cttccgggtc aaggcctcgg acaacgaggg ggtctggaac
    19021 gaggaggccc tggccctgcg ggtgcgggtc ctctccccgc cctggctgag cccgccggcc
    19081 tatgccctct acctcgggct cctggcggga ttgtggtacc tgacggcctc caccctccgc
    19141 ggccgggagt tgagggctcg gatccgggag ctcacggagc tcaagggacg gctcgaggag
    19201 gcgaacgacc gcctttccga cctctccctg caggatcccc tgaccctcgt ggcgaaccgc
    19261 cggcgcctgg acgcggagct ccagaggtcc cacgcctccg cggtgcggga caggcggcct
    19321 ctctcgcttc tgatggtcga cctggaccac ttcaaggatt tcaacgaccg ctacggacac
    19381 cttgccgggg acgaggcgtt gaagtcggtg gcggaactcc tgcggaaaga ggtaagtcgg
    19441 gcttccgact tcgtggcccg ctacggcggg gaggagttct ccgtcctcct gcccaacacc
    19501 gccgaggagg gagcctcccg ggtcgcggag aggatacggt cccaggtgga ggccctggga
    19561 atccccaacg aggccctggg atccgcggcg gtggtcaccc tgagcgtggg gatctcgtgc
    19621 gtcgtgcccg gcccggaatc gacgcccgag gcgatcgtgg cggccgccga cgcggccctg
    19681 taccgggcca aggcggccgg ccggaaccgg gtatccttct gatctccggc cccggcccgg
    19741 accgcgtccg agtaggatgg aataacgaga aatgttcttg ttattgcccc tattccgctt
    19801 gcatgttccg cggaggtgga ttaaaattcc aacgcgcgaa cactttcgcg aaaaagaggg
    19861 agacatgaag agcgtcaagg gaaccgagac cgaaaagaac cttctcacgg ccttcgccgg
    19921 ggagtcgcag gctcgcaacc gctataccta ctacgcgtcc aaggcccggg aggaagggtt
    19981 cgtccagatc tcccagatct tcgaggagac ggcgaaccag gagaaggagc acgccaagcg
    20041 gctctacaag ttcctcgagg gcggagaggc catggtctcc gcgtccttcc cggccggaac
    20101 gatcgccggg acctcggtga acctgatgca cgccgcggaa ggcgagaact acgagtggaa
    20161 gaagatgtac cccggcttcg ccaagatcgc ccgcaaggag ggcttcgatg cgatcgccac
    20221 ggtcttcgag gccatcgcgg tggcggagaa gcagcacgcc aagcgctacg agaagctcaa
    20281 ggccaacgtg gacgccgccc aggtcttcaa gaaggagaag tccgtgacct ggcgctgcat
    20341 caactgcggc tacatcctgg acgccgtgga ggcccccaag gcctgcccgg cctgcgccca
    20401 cccccaggcg tacttcgagg tcctggccaa gaattactga ttcccggaat cgacgcttga
    20461 cgtcaaaagg aggctgatat ggccaaaccg atggaagtct acaagtgcat gaagtgcggc
    20521 aacatcgtgg aagtcctcca cggcggggac ggggacctgg tctgctgcgg cgagccgatg
    20581 aagctgctca aggagaacac cagcgacggc gccaaggaaa agcacgtccc cgtggtcgag
    20641 aagaccgcgg agggctacaa ggtgaaggtc ggatccgtgg cgcacccgat gctcccggag
    20701 cactacatcg agtggatcga gctggtggcg gacgggatct cctaccgggc cttcctgaag
    20761 cccggctcgg cgccggaagc attcttcccc gtcaaggcga agtcggtgta tgctcgggag
    20821 tactgcaatc tgcacgggct gtggaaggcc tagcaggttg ttgaaacact cgaagcattt
    20881 caacaacctg ccatgcgata gctcgggagc cgggcggttc cctgcgctat cgcgagattt
    20941 aggaagctgt cgaaaaaggc gccttcgcct ttttcgacag ccgtagagtc gaggaggagc
    21001 cggaatgcag aagtacgtgt gcgacgtgtg cggctacatc tacgatcccg cggtcggcga
    21061 tcccgacggc aacgtgaaac cggggacccc cttcgagaag ctcccggcgg actgggtctg
    21121 ccccatgtgc ggggccgaca agggaagctt cagcccggcg taagcccgcc ccccgggccg
    21181 accgcggccg gacgaaaccg tcccggcggc cgagccgggt cgttgatgcc ttcgcctctt
    21241 gcggctagaa taggccgtcc cggaacgccg gggcggctta ttcgtttttt caggaggacc
    21301 ttggtgatct cccgtgagga cttcatattc gccatcgggt tcgacggcgc cgaggcggtg
    21361 gtggacggcc gggccaagcg cgagttcggc aagctctcca ttcgggaact gacggaggcg
    21421 ggccagtggc gcgcggcctt cgcggcggcc ctctggtccg gcaaggacga ggatatccag
    21481 gagttccgct ccctctacaa ccgcgcttcc ggctcggact acaagaccgt ggacgaactc
    21541 aagcgcctgt tcggggtgca gaaggaagag atcgcgaagg cgttggtgtt atagcgggtg
    21601 aatgggtgaa tcgtgaataa gtgaatatgg gggatgccac tgcccgccgt tcctattcac
    21661 ccattcacct attcactatt tccgcaatca cacacttcag ataccacgac tccggatacc
    21721 cggacaccac gggatgatcc ggggcctggc cgcggtcctc caggtagcgg aggcgtcggc
    21781 cggaatccgc ggcggcgtcc tccagcatgg cccggaatct ctcccgatcg aaccaccagg
    21841 aacaggagca ggtcgccaat acgcccccgg gttccaggag ccgcagggcg cggaggttga
    21901 tctccttgta gccccgggcc gcaccctcca gggcggcgcg gttcttggcg aaggcggggg
    21961 ggtccaggac cacgaggccg aagcggcggc cctccttttc caggtcccgc aggaggtcga
    22021 aggcgttggc cgctcgaacc tcgaccttgt tttctagccc gttgcggcgg gcgttctccc
    22081 ggacctgggc gacggcggcc tccgaggagt ccacggacag aacggaggcg gcccccgccc
    22141 gggccgcgca gagcccgaag cccccggcgt tgcagaaggc gtccaggacg gtgcggcccg
    22201 gggcgtaggc cgcggcggct gcgcggttct cccgttggtc caggaaccac ccggtcttct
    22261 gtccccccgc caggtccacg acgaaggaaa gcccgttctc ccggatatcg atccggtccg
    22321 gcacggagcc cgcaaggacg ccgacggagg gggaaaggcc ttccagccgc cggaccggtg
    22381 cttcgctccg ctccaggatc ccgtcggggc cgagggcgcc gcgcagggcc tcgacgatcc
    22441 cggccttccg ggcttccgcc cccgcagcca ggacctggag gacgatccag gagcccggct
    22501 ccccggcggg ctcgccgtcc gggccgaggg gcgtcccgac gaagcggtcc gccaccaggc
    22561 cgggaaggcc gtccgcctcc ccgaagacga tccgggcgct ttccgaggaa aggtcccgga
    22621 cccggcggcg aaaggccagg gcggtctcca ggagccggcg ggtgaaggcc ccgtcccagt
    22681 cctcgtcgga ccgggaatgg aggcggaccc ggatcttgga gcccgggctc agggtgccgg
    22741 agcccaggaa gcggccccgg gccgcgaaga ccctcgcctc cgcgccggag gccgccgggc
    22801 cgtcggcgcg ggcgatctcg ttgtcgtaga tccaggggtg ccccgccagg atgcggtcct
    22861 cctcccgggg cttcagtcga atgtccacct tcgcgtccat ggggaaaatc tagcccgggc
    22921 ggggggtgtc gggctagggc tcccttgatc aattgcgaag ggttctatat atttctaccg
    22981 ataagcaagc ggctcacccg atccatggga tgggagggcc gcgggatccg gcctgttcgg
    23041 ctttcgcgga gtttccggga agccgccgta tccgtcgccg cggccccacg ggggtggcac
    23101 ctcgcatcgg aagaccggat ccgacacgct cgctcgtacg aggagtgacc atgaaacgcc
    23161 ccgtcctttg cgcggccgcc gtcctggcgg ttctccttcc cctgtcatcc tgcggaaccc
    23221 gcgtctccgc ctcccccctc ggcctcgccg gaaacggcga cgtggtcgcc cgggagctcc
    23281 gggtttccgg attcgacgcc gtggctttcg gcggagccgg tatcctccgg tttcaccgcg
    23341 cgcccgccca tcgggtggtc gtgaccctgg attccaacct actggagagc ttccaggcgg
    23401 agcttcgcgg ttccacgttg gagctgggct tcaagccggg attccagatc aaccgctact
    23461 ccaagctcga gatcgatctc tacctcccgg acttgaaggg gatcaccctg tccggttccg
    23521 cggaggcggc cctcctggat tccttccggg gttcgacctt ccaggcgaat ctgagcggct
    23581 ccggccgcct gatcggggaa gttcggatgg acaggatccg ggtgacgatg tccggatcgg
    23641 gcaacgtgga gctcggcggg gaggcggacc ggctggaggc ggacatctcc ggctccgggg
    23701 tgatcaaggc cgcccgcctc gaggtccgct cggcccgggc tagcctgacc gggctcggtt
    23761 tcctggatgt ccgggtccgg gacgacctgg acatccgcct gagcggggcg ggccgggtgc
    23821 ggtacttcgg cgatcccaag gtccggagca gcgtcacggg cgccgggacc gtggaacggg
    23881 cgggcgcctg atagtccgcc gaggccccga tccgtcccga aacgggaaga ttcatccggg
    23941 gcgcgtcggc atttttcccc gttcggctgt atactaaggg ctgccggcct tccgccggcg
    24001 gccctttcat ttccggatta tggaggatgc tatggcgcag tcgaagtcgg gtcgatggat
    24061 tctttccggc atcgttagcc tggccctcgg ggccgccgcc ctgacgaccc tggcttcctg
    24121 ctccgcggct cccgcccagc ggccggaaac cgtcgcggcc gcgccggagc ccgagaccaa
    24181 gccctccgac gattactctc ccaaacctcc cgtgccgcct ccggccccgg ggccctcggg
    24241 agacaaagga tccgcggccg cggccgcctc ggaatccttg aagatggagg aattctccaa
    24301 ggaccgagca gcgggggcac ccgcctcgcc gtccgcgccg ggccgcccct ccgcccctcc
    24361 ctccgcctcg ggcctcaagg cgggcttctc ggacgacaac gcccagttca actacttcct
    24421 gggcttcctg gaggagttcg gccacgtccc gaaccacgcc ttggagatcg gggagcgcat
    24481 caccctaagg atccaggact ccgcgggcaa ggccgtgccg aacgcccggg tcgcggtccg
    24541 cgcggggacc cgggtcctgg accaggggct ctcgtacgcg gacggcacct tccggctcta
    24601 tccccttatc tacgagacca ccgccgccag cctctcggtc cgggtggagt cccaatccgg
    24661 ctccgcgagc ctggacgtga cccgggacgg gccccgccgg gtcgtggtca agctggaccg
    24721 gccgcgggtc ctgcccgccc ccctgcccct ggacgtcctc ttcgtcctgg acaccacggg
    24781 ttccatgggg gaggagatcg agcgccttcg ggacaccatc gagatcgtcc acgccaacat
    24841 ccgcgccatg acccccaagc cggcggtccg gttcggcatg gtcctctaca aggaccgggg
    24901 ggacgagtac gtcacccagg tcgtgccctt caccgcggac ctcccggcct tccaggcggc
    24961 cctggcggag gtctacgcct cgggcggcgg ggacaccccc gaagacctcc aggcggccct
    25021 ggaggactcc ctcaagaaga tggactggaa caaggacggg gtgcggaccg ccttcgtcat
    25081 cacggacgcg gagccccacc tggactacgg ccagagctac acctacgccc acgccgccaa
    25141 ggacgcccgg gcacggggca tccgcttcca tacgatcggg accggaggcc tggccgtgga
    25201 gggcgaatac gtcctgcgcc agctggccca gttcaccgag ggcaagtaca tcttcctgac
    25261 ctacggggag accggggagt ccgagggcgg cgccccgggc tcggtgagcc accacacggg
    25321 atccaacttc cagaccgaca agctggaggc catcgtcatc cgcttcgtga aggaggacct
    25381 ggccaacctc tcggacgtgc ccctgtccat ggacgaggac tacttcgagg cgaccaagat
    25441 ccccgaggag gagcgggacg ccaccctgga caagctcttc gaggaggccc tgaggaacct
    25501 cacggactac tcgaccttca aggtggagaa ggacaccccc gccgcggtcc tgcccctggt
    25561 tccgaatacc ccggacctgg cctccaacgc cgagtacttc ggggagaggc tccttttgac
    25621 cgcggtcaag tccaaggcgt tcaagctggt ggagcggaag tccctgcagt cggtcctgga
    25681 ggagctggag ctccagctct cggggctggc ggacgagaaa gccgccgccc gggcgggcga
    25741 actcttgggg gcggacatcc tggtgaccgg ctcggtcttc cgccgggacg ggaagttcga
    25801 gatcttcctg cgcctggtcc gggtctccac cgccgaggtc ctgtccgtgg cccgggcccg
    25861 ggtggacgcg aaactggggc tgtgacggaa cggtttccgg attcaaccac ggaggtcacg
    25921 gagagcacgg aggaagccgg gcttggaacc atagatccga caggatggaa aggatgaaga
    25981 aaggatgaac aggatggaag cttgggatcg gatattccct atggtgtatc ctgtccatcc
    26041 aggatttgat cctgttcatc ctgtccgctc cttggatgtg tagtctttct tcaccgtgtc
    26101 ctccgtgtgc tccgtggtga attcctgaaa aatgctgata ctatcgacag atggataagg
    26161 ggagaacccg aatgcaagtg gtttcgtcgg ctccggcggt agttccggca tgagcgcgca
    26221 gaagaagaag ccgaccgtct tccgttgttc ctcctgcggc cacgaggagg ccaagtggct
    26281 gggccgctgt ccctcctgcg gggagtggaa caccctgcgg gaggcggcgg cttccgcctc
    26341 gtccgtccgc gccgcgggag ccgcgggcac cgccgagagg tggtccctgc ccctggccgc
    26401 ggtggacccc gcggagggat cccgcttccc ttccggcatg gccgaggtgg accgggtcct
    26461 gggcggaggg gtcatgaagg gctcctcggt cctggtgggc ggggaaccgg ggatcggcaa
    26521 gagtaccctc cttttacagg tggccgccaa agcgggggtg tccggccggg tcctctacgt
    26581 ctccggggag gagagcgcgc cccagatccg gctgcgggcg gaccgactgg gcctggcccg
    26641 ggagggtctg gagatcctct gctccgggga cctggagctg gtcctggacg ccctggaaaa
    26701 gctcaagccc cgggcggtgg tgatcgactc cctgcagacc ctgaggtccg cggaggccgg
    26761 gagcgtgccg ggcacggtga accaggtgaa gtactgcgcc ctggccctgt ccgagtgggc
    26821 caaggaggcg ggctcggcgg tcttcctggt ggcccacgtc accaaggagg gggccatcgc
    26881 gggccccaag gccgcggagc acatggtgga cgcggtcctc tacttcgagc agaccgacgg
    26941 ggagctccgc ttcctccggg ccacgaagaa ccgcttcggg tccacggacg aggtgggcct
    27001 gttcacgatg accgagacgg gcctggcgga ggtggcggat ccctcccggc tcttcctgac
    27061 ccgccgggag gggggcctgc ccccgggcgc cgccgtggcg cccctctggg aggggagccg
    27121 ctgcctcctg gtggagatcc aggccctgac ggtccccgcc aaggcctccc tgacccgggt
    27181 ctactcggac cgggtggacg cggggagggt ggcccggatc gccgcggtcc tggagcgcca
    27241 cgggggcctg cggttctcgg accaggacat ctacgtgaac gtggccgggg gcatgcgcat
    27301 cgccgagaca ggggcggacc tggccgtggc ggcggccctc tactccgccc ggtcgggcct
    27361 gtccctgccg gaccggtgcg ccgtggccgg ggagctctcc ctggccgggg aggtccggcc
    27421 cctgcggcgg atgcgggccc gggtgcgggc ctgcggggcc ctggggtatg ccgaggtcct
    27481 gggtcccgtc cccgatgacg gggagaggga gcccgggtgg accggggccg ccacgatcaa
    27541 ggaggcgatc cgcaggctct tcgggccggg ggccagaggg gcgtccggcg gaaagcccgg
    27601 gccggagggg aggggggaat ccgtatgaaa ctgtccatcg agacccggaa gtccggagtc
    27661 gtggtgcccc tgggggccct tcggaccgcc cgttccggcg gagtggggga gtatccggac
    27721 ctgccggtcc tgggcgagct ctgcgagcgc tgcgggctgt ccttcgtcca gctcctgccg
    27781 gtgaacgaca cgggcttcca gagctcgccg tactcggccc tgtccgcctt cgccctccat
    27841 cccctttacc ttcgtctggc ggacctgccc gaggccgcgg ccttccgggc ggaggcggag
    27901 tccctggcct cccgcttcgg agggaggaag aggttcccgt atcccgagat cctgtccgcc
    27961 aagctcgcac tcctgcgccg gatcttcgac accgccctcc cggcgatccg gaaggacccc
    28021 gccctggccg cctggatcgg ggccaacccc tgggtccggg actacgccgt cttcctgggg
    28081 ctcaaggagg agcacggcgg gaagtcctgg aaggactggc ccgcacgccg ggatcccggg
    28141 gccggggaca tcgaggccct ctgggaagag ccgggccgcc gggacggcca cctcttccac
    28201 gcatgggtcc agctgcgctg cgaggagcag ttcctcgcgg cctcccgggc cctggagaag
    28261 cggggcgtgg ccttgaaggg ggacctcccc atcctgatga acgacgactc cgcggacgtc
    28321 tgggcccaca ggtcctggtt ccgcacggac cggatcgccg gtgcgccccc ggacatgttc
    28381 tccgaactgg ggcagaactg gggcttcccg atctacgact ggggcgccct ggcccgggaa
    28441 ggctattcct tctggaccgg gcgcctcgcg caggcggcca agttctacgg ggcctaccgc
    28501 atcgaccacg tcctgggctt cttccggatc tgggccctcc cggagcggga ggagtccggc
    28561 tacctgggag ccttcgaacc cggagccgcg gtctcccgcg ccgagctcgc ggccgcggga
    28621 ttctccgacg gccggatccg ctggatctcg gagccccaca tccgggaggc ggccctgtcg
    28681 gaggccgcga gccggggcgc cgcggcggcc gtcgcggccg aggggatcgc ggcccgggcg
    28741 gcggaggccc tcctggaccg catcggctcg gagccccttt tcctgttcaa gcgatccgtc
    28801 cggggggaga aggacatccg ggcggcgggg cttccggagg ccctccggga cttcctccag
    28861 gccgcgtgga gggaccgggt cctcattccc ctgggcccgg actccttcgc cttcgcctgg
    28921 cgtcaccctt ccgcctccgc gtggcccagc ctctcggatt ccgagcggtc cgccctggag
    28981 ggtctgctcc gggccaaggc cgccgaatcg gagagggagt gggaggagca cggccgccga
    29041 ctcctgtcgg tcctcaagga cgccgcggac atgctgccct gcgcggagga cctgggggcg
    29101 gtgccccctc tcgtgcccaa ggtgctagcg gaactcggga tcctgggcct gcggatcccc
    29161 cgctggaccc gccgctggga cgagcccggc cagccctacg tcgagcccgg ggacttcccg
    29221 aggctgacgg tctcggcccc gtccgtgcac gacacctcga ccctgaggga ttggtgggaa
    29281 cacgaggccg ggcccgcgga ccgtacggcc gcctacgggg cggctccgcc ggaatcctgg
    29341 ttgcccgagg tcggggcccg ggtcctggcg cggttcgccg cgggggcttc ggtcctcctg
    29401 gtggtccagc tccaggacct cctggacctc tccccggcct acgcctccgg ggactcccgg
    29461 gccgaccgga tcaacgtccc gggcaccgtc gcggattcga attggacctg gaggatgccg
    29521 gtcactctgg aggatcttgc cgccgacccc cagtgggccg ggagggtggg gcgctcggtc
    29581 gaccggtccg cggcgaggcg ggcggcctct ccggccgcgg ggccctagga accgcgggtt
    29641 ccggagcgga agggccggac ggccgtcccg ggccggtccc ggcctgcccg gcgggagccc
    29701 cggcggccag ctcgtagagc aggacggccg cggactgggc cacgttgagg ctctcgaggt
    29761 cccccgtgcc gggtatccgg accagggcgg agcaggccgc ccgggaggcg ggggagatcc
    29821 ccgtctcctc gttgcccagg accagggcga cggcccggcc ggtcggcagg gcggacaggg
    29881 cctcccggac ggtccgggaa cccctatggt cggcccccag gatggccagg gacggaccgg
    29941 cccggcgcgc gagctccccg gcgtccgatg ccccgtacag gttgacccat tccatcccgc
    30001 cctcggccac ccggaaggcg ctggtggtga ccagggcggc cgcggagggt ccggccagga
    30061 ctacgttgcg gacgccgaag aaggcggcgg agcggacgat ggcccccagg ttgtggtcgt
    30121 tccccagcct gtccaggacc aggatccgct ccccccgggc ggcccagccc gcggcggtcg
    30181 cggcgtccag gtccggcacc tcgggggcct ctatcatggc cacggccccc tggtggtgga
    30241 ccgtcccggc caggcgctcg agctcctcgg ggctcaccat gcggtacagc gtcttgcgct
    30301 cggcgaagcg gcgcaggacg gtgcggaact ccggcaggag ggactcggcg aggaaaaagc
    30361 ggcggacgcg gtcgggattc cggtccagga gggcccggac ggcggacagt ccgcagacgg
    30421 caagttcccg ggtgagcttg tttttcacga atccatggta cttcgggcac ggcccccggg
    30481 gcaagcgcgt cccggatcgg cttgtcatcg gcggccccct cggcgtagta tggacgggta
    30541 tgacagacgt acaggtttcc gccgtcaacg aggagacgat cgacacgatc ctggccgggg
    30601 acgtggagtt ctcggggacg atggtcttca agaagcccct gatgattaag ggcagggtga
    30661 gcggcacgat ccgctccgag agcgacctct acatcgacac gggggccgtg gtggaggcgg
    30721 agatttccgc caaccaggtg tccgtgcgcg gcaccgtcaa gggcaacgtg gtggccaagg
    30781 accgggtgga cctctacgcc tcctgctccc tggacgggga catccgggcc ccggaggtga
    30841 ccatggagac gggctgccgg ttcaacggga tctgcaccat gaccgggaag aaggcggatg
    30901 cgccggcttc ctgatccccg gatcgccttc ctggccctga tcctggccct ggcgaccgga
    30961 ccggcttcgg atctcggcgc ccaaacaccc cgtccggatg ccttgaagct ctaccgggag
    31021 ggccggtacg aggagtcccg ggtccagtgc ctccaggaaa tctcccagga tcccgccaac
    31081 atcgaggcct acgtggtcct gggttggagc ctggtggccc tggggcgcca cgcggacgcg
    31141 gaggtctacg cccgcaaggc ctacgacgag gtgcgcaagg atccccgggt catggagacc
    31201 ctgggcgagg ccgcctactt cctgggcaag aacgaccagg ccctgcgctg gttccagtcc
    31261 tacatcacgg cccttcccga gggctcccgc gtcgcctcga gctaccagta catgggggag
    31321 atctacatcc gcacctcccg ctggggccac gcggacatcg ccctgcgcac ggccctccag
    31381 ttcgagccgt ccaacgcccg gtggtgggcc cgactgggct acgcccggga gatgggtggg
    31441 gacctgcgct attccctgga agcctacgac gcggccctga agatcaaccc cggcctgacg
    31501 gacgcccagc tcggccggga ccgcgtgtcc aagcgcctcc gctgacgtgc ccttccgcgc
    31561 cgccttccgc accttcgggt gcaagctcaa ccagctcgag accgaggccc tcgcgggagc
    31621 cttccgggaa tccgggaccg tcctggtccc gttctcggag agcgcggacg tctacgtcgt
    31681 gaacacctgc accgtgacgg gcaaggcgga gcagaaggcc cgacgggaga tccggaaggc
    31741 cctgcggtcg aatccccgcg ccgtggtcct ggtcacgggc tgctgggccg aggtggggac
    31801 cgaggaggtc gccgccctgg gcccccggac cgtggtgatc cccgggagcc ggaaggacga
    31861 cgcggccctc ctggcctcgg acctggcgga ggcctgggag aaggggatgg acctggcgga
    31921 ggccgcggag cggggccggg acgcccgggc cggccgagcc cacgacccct tcgccttccg
    31981 acccgagacc ttcctgctcc actcccgggc ctccctcaag atccaggacg gctgtgacaa
    32041 tcgctgtgcc tactgccggg tctgcctggc ccggggcccc tcccggagcc tcgagcccgg
    32101 cggagtcgtg gagcgcgccc gggccctggc ggtgcggggc gcccgggaaa tcgtcctgac
    32161 cggggtgaac ctctcccagt accgggccgg gggcctgtcc atggcgggcc tccttaccct
    32221 cctggtccgg gaaaccgagg gggtctccta cagactgtcc tcctgggagc ccgacaaggt
    32281 ggacgaggag ttcctgacgg ccttcgccct gccccgggtg cggaaccacc tgcacctggc
    32341 cgtccaatcc ggctgtgacg ccgtcctggc ccggatgggc cggggctacc gctccgagac
    32401 cgtgcgccgg gcggtccggg acctccgggc cgccaaagcc gaccccttcc tgggggcgga
    32461 cctgatcgcc ggctttccgg gggagacgga agccgagttt caagccacct tcgatctgtc
    32521 cgcggagctc accttcgcct ggatccacgc cttccccttc tcgccccggc ccggcaccaa
    32581 ggccttcggg atgcggcccc gggtgcccga gcggaccgcg ggagcgcggg tggaggccct
    32641 cctggacctg ggacgccgga gacgggcggc ctacgcggcc cgggctgtcg ggcgcctcac
    32701 ccgggcggtc ctggaggacc ccgcggagga ggacggcgcg gaagcgtccg gggtcgaatc
    32761 cgagtacgaa cccggaccgg accccgcccg ggaggccgcg gtcgcggcga tctcggacga
    32821 gtacctacgg ctggaggtga cggggatccc ggagtccctg cgtcgccggg ggtccgaggt
    32881 ctcctgccgg atcgtggagg ccctgcccgg cgccggggac ctgggaatcc cggacgcccg
    32941 ggccgaattc gtcggtccct gagcccggga tcctggaaaa aactccattc tgatatatac
    33001 tctcctgtac cctcgtccaa cgtttccgga ggcccgcatg cgccgaatgc ttccagtcct
    33061 gatcctcgcc cccctcctcc tcctgggcgg ctgcgacgcc ttcttcacga acgtgttcga
    33121 aggcctctac accctggagg tcccgagcgc ggccgaactc ggtaccctgc cggtatccga
    33181 cctggagcgc ctggcggagg atgaccagtt cttcgaggag ttggccgggg atcccgcgaa
    33241 ggaagatgcg gttctggaaa acctcgacaa ctatttcgga cccgaggggg acgggaccgt
    33301 ggacactccc gaggaacagc gagccgcggc cctgtacgcg gaggtgttgc tgcggacgtc
    33361 gggggcggac cgggtcgtcg cgggtctggc ggggtacctg gcctccgcgg acgggttcga
    33421 ccagctcgag acgaatcccg gtccggaact tgcggagctt ttcggagaat ccctccggac
    33481 cctcgccgag atcgaggcca tggtcgcggc cttggacgcg gcctgggacg cctacgcggc
    33541 gatcggcgcg ggcctggcaa catcccccct ggacgagtcc ctgaacgccg gcgacatcgc
    33601 cttcggcgcc gccttagccg cggccctgaa gggggttacg gtgacgagcg gccaaaccga
    33661 tgccgagtat atttacgctt caattcaagg aaccgcgggc gggatgagcg ctgatttcag
    33721 cacaactgta ctgtcggaca ccgagatgga ttacctattc ctggccgcgg gattcgaccc
    33781 cgcggacctg ggcctctaga ggagcggcgt acatgaagcg catcggaatc gtacttgcag
    33841 ccgccgtcct cctcctggcg cccctgacgg cccaggacat ccccctctcg ggcttctctc
    33901 ccaaggacgc ccgggccctg ggcatgggcg gggccttcac cgcagtctcc gaaggctttg
    33961 cgagcctcta cggcaacccc gcgggcttcg ccgcctccga gggcgagctg accatcctgg
    34021 ccccgatgtc ctggctgtac ctccgcccca cctcggacaa cctcgccctg ctccggagcg
    34081 ccgtcgccga cgatggcgcc gcggtggacg ccctggaaac cctcctgacc gacaacggcc
    34141 tggggggcgg ggcgagcctg gggatcggct acgtcgggaa gggcctgggg atcggggcct
    34201 tcgtgaccgc ggacgcctac gccgccgggg acacccttgc cggcgccgtc ctggacgtgg
    34261 acctccagtt caacgcggtc ctgggcctgg cccggaccgt caaggtcttc ggcctgggcc
    34321 tgaccctggg cgggaacctc cggcccttct accgcgccgt gggggacatc cccctttccg
    34381 aggtcgtggg ggcgatgatg ggcaccgcgg accccggcga cgtcttccta tccaaggagc
    34441 tgatctccgg cttcggcctg gccgcggacc tgggtgcttc cctcgagctg ggggcgttca
    34501 agctgggact ggccgtgcgg gacatcgcgc cggccttcca ggcttacagg aacaccgtcc
    34561 gggacctggc ggactccggt ttcgtgactt cctcgggaac gccggcgggc tccttcgccc
    34621 tgaccccgaa cgtggtcctg ggcgcgagct ggcaccccga tctgggggcc ttctcccgga
    34681 tcctgaaccc ccgcgtcctg ctggagatcc aggatcccct ggccgcgatc cgggaccggg
    34741 cgtccttctg gaacctggtg cacgcgggag tcgaggtcaa gaccctgggc ttcctggccc
    34801 tccgggccgg ggtcaacaag ggctggctgt ccgccgggct cggcctggac ctcttcttcc
    34861 tggagatcga cgcggcggcg ttcaccgagg agctgggctg gcgaccgggc cagaacgggc
    34921 ggagcggcgt ggctctccag gcggccatcc ggctgtaaca ggccgggccg ctctggcggg
    34981 acgcgaccgc ggaatgctag agtctcagga atcggcccct cgcggccgat actcgtagga
    35041 ggcccggccg ggcccgaacc cggcgggagg gacccggcgc tccgggcgcg ggaggttgtg
    35101 catgaagcgg acgaggtaca tcgcgattgt tgcggccgtc ctggccgccc tgaccctggc
    35161 ctcctgccag gacttcttca cgtcctcctt cgcctcctgg gcccagcggg atccctcggt
    35221 gccctccggc ctgacagcgg accaggcgat gagcatcgcg gaccaggccg tggcgaaccg
    35281 cgacaccgcc ctggctcaag cactgttacc gcagatggcg ggtttgatcg ccacaggaac
    35341 gccgccggcc gccttggtcg cctccgccgt ggatacggct gtgttggctt cgggaataga
    35401 cgatgcgttc ggaaccatcc tgacggacat cggaattgaa gcgctgacgg acgatccgac
    35461 cgtgtatgcc gcacaaatag atacgatcat ctccagtatc gcggtcgatg tcgattctca
    35521 ggcgatcttc acggctcttg cggccgccga cccgaatgta atggccgctg caggagccac
    35581 agcttccgat tatgcgacag cagcggtagc tctagtactt gcggaggccg ccgctaattc
    35641 aatagatttt ttctcggact ctccggatat tactaccttt acttgctaca gcacggttga
    35701 gatgcttatg gacaatgcga gtgctacatg gcctaatgat tcaattgttg atgttctggc
    35761 agattttatt tcctatgtac cttaagcagg ggaaggcagt gaatataaag tctcgttctt
    35821 tatccatgtc aacttttgct ttgacttacc ttctgctcat cctctccctc cccctctccg
    35881 cccaaaccgc cgcacccggt gtacccgttt ctccccgcag ggccgcgatc gggggcctcc
    35941 acgccgcgga cctcgccgga ttcgattcga tcttcacgaa tcccgcggga ttagcgtccg
    36001 cggagggcga gtggatgtac tcccggatcg ccttgaacgc cgcggggccc atcttcgaca
    36061 tcgccaacat ggccctgggc ggcacggaca tcctgtccaa cctgaccagc cttctggacc
    36121 cggcgggacg gctgtatact tccatggacc tggccacgcc cgtcgccctg ggctacgtcg
    36181 gcaagggctt ggggatcggt atcttcaacc ggacctacgt gatcgtcaac gccccctcgg
    36241 tgacgacttc cttcctgact tttggcgagg aactcctcct gaccggcggc tacgccaagc
    36301 gcttcgatct gggggaagga aacctcctgg acctgggcat cgtggtcaag ggcttcctcc
    36361 ggaacgagtt cgcctggtcc ggggcgctca cggacgtctc caccgccttc aacgacatct
    36421 tcgggggcat ccccttcaac atcgtgacgg ggatgggcgt ggacctggga gccacgtgga
    36481 ctttcggaga ttacgccctg gccctgactg cccgggatgc tttcacgccc tacatcgcat
    36541 cgtcctacac cagctacaac gggttcaagg ccaatcccac ggccaacaag atcgagacgg
    36601 tcaccgacct catgcccatc gacctgtccc tgggcttccg ctggaacccc cggtggctct
    36661 tcctggcccg gacgggaagc gagcttaccc tgctggcgga ttaccgcaac atctgggggc
    36721 tcttccggat cctggaccgc aacccgatcc tggagctcag cctgggcacg gagatcgtca
    36781 tgctggagat cctgagcctc cgggccggaa tacgggacac cctgccctcc gcgggcttcg
    36841 gcctggacct ccaggtcttc gacctcagcc tggccatgta cggcaaggag ctgggcctgg
    36901 agcccggcca gcgccccgtc ttcaacctgg acttcgcgat cgatttccgg tactgaagcc
    36961 ttacgggcat tttcccaacg gactcctggt tcctgagtcc cgactcctcc cctgccgcgt
    37021 tgacactccc cggttccttc cgatagtcta aaagcccgtt tcgggccgct agctcagtag
    37081 gtagagcaac gcccttttaa ggcgtgggtc actggttcga atccagtgcg gctcagaaaa
    37141 tccgcgctcc ggtgctgccg gggcgctatt tttttcccgc actggattcg aagggtgcgt
    37201 gcgccgagct tcgcgcgaat cgatgagcgc gaaacgaggg aaaggcgcta tgagggcgtc
    37261 cgccagtgcg cgccccggcc cggagaaccg agacaaggat gccggggtcg cgcaagcgac
    37321 cactaagatc cattcgcatc ggcaaacgac gcgaagcggc gtatgtcgag gttcgcaggg
    37381 cgaatccagt gcggcttatt cagtggacgg agaacggtgg acggtgaact ggggaagcac
    37441 gggtttttag gttggccgga atacccgggg cgggtgtcat gtattctcgt tacagctcac
    37501 ggcctgctca acagcctgat ttccctcgca agttcgtagt tgccatccgg tattccggga
    37561 tcccggaacg ccgaaatctt gaccatattc tcgtagaggt cgaagagtag gtaggtgctg
    37621 ggcgcgcctg catccgcgtc gccccttccg tgaccggcgt cgatctgcgg aaccccgtct
    37681 acaagagctg cgctgaagtt gtgtgtatgt ccgcagacgt atgcggtcac gctgtactct
    37741 tgaagcaggg cgatgaagct ccggacctca acaggatggg cgtccagact ggtaccgtcg
    37801 tgacggaggc gtccggtggc ttcatccgcc ataggccaga ggggttcgtg accgaagacc
    37861 aggatgaagg cgggagcgcg gaggttcgcg gactccaggt cggtccgcag ccattcgagc
    37921 aagctcggag aaatggtgcc cgcaaaagga tccgagggcg gtggatccag gccgatatcg
    37981 gtgaatccgt cataatactg gttgaggaat acgaggtggg caccgccgaa atcgaaggaa
    38041 taacaggtct cctccgaccc cggaggtcca ccgaggaaat tttccacgga atactgcgtt
    38101 tcgaccggga aagccctcag atagtccata tcctccggag tctccgcttc atggtttccg
    38161 atcataggga accagggaaa cgaggagccg agtttttcct ggatggcgcc atatacctga
    38221 tccggagggt cgatgtcgcc ggggctcacc aggaattctc cggcgccttt cgcagtcagg
    38281 gaatccagga ggccgcggaa ttcggtcagc ccgacgaagg atctcatgtc cgcggctacg
    38341 gagaatcgga ccggcgttcc tgcgggatcc tccacgattg ccgtaatttc acttatggac
    38401 ggagcgaaca tgtccccggc cgggcaggat gttaacacaa gggccagagg cgtgatgatc
    38461 gagagccagc gagcgaaggc gggatttcgg gtcactaggg cctccttttc cggatgttat
    38521 acggcacggg attcccggcc gtgatctcaa cccctacctc acctttccca cgaagctctt
    38581 caccgcagtc ttttccaggg agtcatcggt ctccttggtg gccatggaca cggcgtccac
    38641 gggcttggct ccgttcttct ccaggaggcc gttcaggtcc ttgaagaggt tgtccaggaa
    38701 ccagcggttg gcggtcacga agaggtagac ccgcttgccc gcgatcggcg gcttggtctt
    38761 gagccagtcc cggatggggg acttcgagtt gaaggcttgg acgatgccgt gcaggaccag
    38821 gacttcatag cgggagagat ccttggactc gaggtccacg gccgccgcct cgtcgaaggg
    38881 gatgccggag gaggtgaagg cttcccggaa atgcgcgatg tagggcttgg tctggtcgtt
    38941 cacctcgtcg tagacgaaga ggacccgggg cgtcggggct tgggcggaaa ggggggctat
    39001 ggtcatggcc ataaccgcga ttacggttag acgggcgcgg cggcagagtt tcatgaaagg
    39061 ctcctctgcg agaagtatac cccgcggagg agcctttcaa aatatgatcg cggatcagga
    39121 gactttttcg gagtcggccg cctgttggcg gaagtccgca gggcttacgc gccgccgcat
    39181 gcttcagtcg ctcccgtcca ccgtccactg tccaccgtct acttcactcc caactcgtcc
    39241 tgcaggaagg cgatggtctg cctccaggcc tggcgggcgg gaccggggac gtccacgttc
    39301 tcgtccttca cgaaagcgtg acctactccc gtgtagaccg actcggcgta ccgggccttg
    39361 cgggcggcca gggcgtcctt gaagccctgg cgctgggcgg gagggatgcc cgcgtcggta
    39421 tccccgaaga tcccgagcac ggcgcccttg cgcccgagct cgcccagggc ggactcctgc
    39481 tggatgaggc cggtgccgta gagggtcacc accgcgccga ggcccgcctg ccgggtcccc
    39541 aggagcatgg cctgggctcc gccgaagcag aatcccaatg tacccatgcg ggatccgtcc
    39601 acccggggat ggcgggtcag ccactcgtag gcggcgtcca ggtccgcggc gatctgcgtc
    39661 gggggcgtgc cgttccgcag ggctcccgcc tccgcggcgg tctgggccag cttgccccgg
    39721 taggcgtcca cggccagtac ggcgaaccct tccttggcca ggcggtccgc catggcggcg
    39781 atgtccccgg acaggcccca ccactcgtgg accatgatga ccccgggatg cttcccgaag
    39841 ccggggggga gggccaggta ggcgcccagt ttggtgccgt ccgcggcggt gaaggacgcg
    39901 ttggtctgca caggcttctt ggcctgggcg gccagggggg atacggccag gagcagcaga
    39961 gctatggtga ggatggatac cgccgtgatt cgggaggggg atttcatgaa aacctccggt
    40021 cgataggaat gggacccggc tctcggccgg gattcatcgt ctaaaggtaa ggataataga
    40081 ttataaaagc aataagaaat atttgtcctg gaggttccgg atctgccgat ccgccccgtg
    40141 agccgcgcta gcgcccgggc ccgagtttct cgttcaggac ctttagccgc gagcgcaggc
    40201 ggcgctctcc gatgcccagg gcccgggcgg cctcgctccg gtttccaccc gcggcttcca
    40261 gggccttggc gatcaggtcc agctcgaaac ggccgacgac ttcgtcatag gttccggaag
    40321 cggctccttc cggttccgtc gcgcccgacg acgattccag ctggatgtcc tgggtgctca
    40381 ggatctcccg acgcgtcatg agcacggccc gttcgacggc gttctcgagt tcccgcacgt
    40441 tgccggggaa ggggtgccgc atgagcgccg acagggcttc ccggctgatg ccccggacgg
    40501 gccgcccgta ttccttggag aatttctcga taaaccaacg ggtcagggcg gcgatgtcct
    40561 cccggcgttc ccgaaggggg ggtatcgcga tcgggatcac attcagtctc cagtagaggt
    40621 cggcccggaa cgttcccagc tccatcatac ccttgagatt ccggttggtg gcggcgatga
    40681 tgcggacatc cagggtccgg gtcgcattct ccccgacccg ctggatttgc ccgaactgga
    40741 gggcccggag gagcttcacc tggatgccca gggggatgtc ccctacctcg tcgatgaaga
    40801 ggcttccgcc gtcgccctcg gcgaaccgac cttcccggga ctggtccgcc cccgtgaagg
    40861 cgcccttcac gtgtccgaag agctcgcttt ccatcagggt ctccggcagg gcggcgatat
    40921 tgaccgtcac gaagggcttg tcccgccgat cgctggaatc gtggatcagt cgggcgacca
    40981 gctcctttcc ggttccgctc tccccggtga tcagcactgt cgccgagctt tcccgggccc
    41041 ggtacaccgc gctcaggacc tcccgcatcc gggtgtcggc gtggatgaag gggacctcgc
    41101 tcgactccgg gctcaggaag ggatccaact cgtcgttctc gtgttcgatg gtcgatttct
    41161 cgtactcccg gatgaacagg tgctccaggc tttccgaggt caccggcttc acgaggtaat
    41221 ccgagccgcc ggcctt
//
LOCUS       DAOTEI010000005        40241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_659_length_40241_cov_5.401807, whole genome shotgun sequence.
ACCESSION   DAOTEI010000005 DAOTEI010000000
VERSION     DAOTEI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 40241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40241
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_659_length_40241_cov_5.401807"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..1801
                     /locus_tag="P5313_00885"
     CDS             335..1801
                     /locus_tag="P5313_00885"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY78945.1"
                     /translation="MLRSSFRKAFQARSREAVLLLVRMPLTEVNRPALAAAARRASVE
                     ALLAVRFPLVRLCHGISVLASETSGPGIPEGATRIRALRAALNAAEAEAGLAEGSGAT
                     ASDTPGMSLSGCPCSRVVPVRGRLPLSSSLIRAAVSSGAGLRILRFRPSGLKGTPQDL
                     SDRLSDLLRREALLGLYAIAGRRPDSPAREFALFVPDRPPEASGGGRGSDPLAGFPVT
                     GRIRTLVDAGAEAPSDLAGFELADGDPGTKPSGPAPIGKVEGPPAEKRPAKSAAPVRR
                     EARVAEYRNAAVLRAEIPDLLGALKGMDPREAAAYLNRVLARLASCVEAAGGIVDSCA
                     GGGIRAFWGAPASTGRDAEAAGKAALAMRRTIGALNRGRLASGKVPLRLFCALDAGPV
                     LAAPIGAPDRPAYTAAGEAPRRAAGMGHRNGRLGTDILVSEYAVRLMGAAFRFQPVDR
                     LEAGGEAGTVGIYALLGRSGDASGPRDLGDLRRRLGTV"
     gene            complement(1960..2454)
                     /locus_tag="P5313_00890"
     CDS             complement(1960..2454)
                     /locus_tag="P5313_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRY78946.1"
                     /translation="MEHLLAAVKKGLKGELDSVTIYMEAAEKSDGPVAEFFRERGKEE
                     QRHYNWLLSYYKELSQGRMPSENLAAEVFGMEGRSPLVTEEFLKRVGSSQHLVTAVAA
                     GVLLEVEAVRHYRKAAEETVVPALRTFFDTLADWEQQHYKDLLAIQDEAERYWFDAQR
                     FEPF"
     gene            2535..3605
                     /locus_tag="P5313_00895"
     CDS             2535..3605
                     /locus_tag="P5313_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRY78947.1"
                     /translation="MSIEQFAQGVAFLASRSPVFGEKPLSVTLIANPRAGGFTRPFVF
                     SRHLGELKLLARRAADLPVRGAPTTFRLHATAYPRHAAEVTRALAEEARAAPETERLV
                     VTAGGDGTTLEALSTLMDLSEAERSRFVVLRLPMGTGNDGADSRELLSALELLLGPSR
                     FDRQSALRVIPASAGKPGGRGPWWSFNIASLGADAFVSDMTNRLKLTFPGDSYKLWVE
                     LATVFYDALWPTRGFEVRAWDAKGREVKSFRGDLLLLAMGVSGRRTYGSNKPILPDDD
                     DVCLVRQMPLLRKLVVKGPLTSGRHRGYPEVDLFSASKVQIGFRHRALVQMDGEAERL
                     EAEDFPVVFERTEPAIRVLRRA"
     gene            complement(3659..4579)
                     /locus_tag="P5313_00900"
     CDS             complement(3659..4579)
                     /locus_tag="P5313_00900"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HRY78948.1"
                     /translation="MEPDYDRILADIAQEVRPLLALGAPADYIPALARVPRDRFGMAV
                     RTLEGRIHAVGDADEPFSIQSVSKVFTFILALRSAGDGLWDRIQREPSGDPFNSPILL
                     EQERGIPRNPFINAGALVVADILLEALEDPREELLRFVRARSGSGAVRADPEVAESER
                     STGHRNAALAHLLKSFGNLRNRVEEVLDFYFFQCSLRMSCRDLAASFLFLANRGLCPV
                     SGERVLEPSPAKRAAALMLTCGTYDAVGDFVYRVGLPAKSGVGGGIAAILPGRLAAAV
                     WSPGLNERGNSLAGTQALELFTTKTGMSIF"
     gene            4659..5783
                     /locus_tag="P5313_00905"
     CDS             4659..5783
                     /locus_tag="P5313_00905"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRY78949.1"
                     /translation="MILPENPSAGPFPELAIDLDVLSSNVRSLAGLCSARGVSLTGVV
                     KGADGLAPVARAMLTGGCEHVASSRIGQLRALREAGIEAPLLLVRVPAPGELAETARW
                     ADRSLHSDLSVLRALDREADRAGRPHGVVLMLDLGDLREGWYEEGDLVEAAVRTERDL
                     SALELLGIGTNLGCYGSILPTPENLGRLARAAGRIEERIGRALEVVSGGATSSIPLLL
                     SGALPAGINNLRVGEGLLTARDLPHFYKVDVPGLSPGVVTLRAEVIEVRTKPSHPVGE
                     IFIDAFGERPTYVDRGPRRRALLAVGKQDFGKHESLVPLEPGVEVIGSSSDHLICDVE
                     DLGRPLAAGDILEFGLFYPALLFASQSRYVRKTFRGGGQD"
     gene            complement(5706..7199)
                     /locus_tag="P5313_00910"
     CDS             complement(5706..7199)
                     /locus_tag="P5313_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY78950.1"
                     /translation="MNTTLRGRLTLSHALLAAAAVLAAGGAARLVARERFDAYIAAGI
                     ESRVHALAASVSAARDPGGGWDHGRMESLGVGALEDGFILRLLDGTGSVLWDAREHNA
                     GLCSQMIESMAVRMAGADRGGGYRERSVALPSLPGDAAPPALVAGYYGPVYYRDAELA
                     FLRSLDRVLAAVGLGLAALAVLAGAWSARRLSAPLRSAARAARSIGRGDFDIRLPPSG
                     IRELDELSGALSRLSGALGEQEELRKRLVADASHELRTPLASLRARLEALLDGVRTPD
                     EAALEACRRDALRLGSLVGSLEGLARADAAAYSGAPETFDLAAAVRSVCAAYRDSARA
                     GNLDIRTDAPEPVPVSGDRPALEGAVGNLLSNALRFSDPGGTVEVRVRLARGDGDLPE
                     PAPAPGTRAWAVVEVEDEGIGISPEDLPRVFERFFRADPSRSRDTGGFGLGLAIVRAA
                     ARAHGGEAYAESPGPGRGSRFLLVLPAAPEGLPDVAALGGEEQGRVE"
     gene            complement(7196..7870)
                     /locus_tag="P5313_00915"
     CDS             complement(7196..7870)
                     /locus_tag="P5313_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY78951.1"
                     /translation="MDRVLVVDDEPGIRETVRAYLERDGFAVDEAADGPTALARAETG
                     EYALVVLDLMLPGLSGREVCRRLRAESAVPVLMLTARSGPEAAAEGLDLGADDYMEKP
                     FSPRELAARVRAVLRRARPPGSRLEFPGLSLDREGRTACRDGACVELTASEWRILEAL
                     ALRPGRVFSREEIAARALGELWDGYERTIDAHVKNLRRKLDEDSENPRWIETVRGMGY
                     RFRREP"
     gene            complement(7876..8580)
                     /locus_tag="P5313_00920"
     CDS             complement(7876..8580)
                     /locus_tag="P5313_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78952.1"
                     /translation="MNPVSRHPGHLGAPSFPSLILAVLAAAFLAVSCGSTDVVAKYAA
                     RSFGTAAGALGSPSPGEYRVLASPGGEELRLSADPGSPPRAVLSLDAVPFLEAGLDGA
                     RLPSGPDLSWTVEGGRLTGRFNLGASPLGGKPGGSPADHMAALAASARDRIGYHAQLG
                     HYGLSLGGGNLVEWAAEPAKNDKDWVLILDPAFLAAAGADPSRVRGWTLAKVPVDGPD
                     GKMVEVEKLLLPYDLR"
     gene            complement(8642..9007)
                     /locus_tag="P5313_00925"
     CDS             complement(8642..9007)
                     /locus_tag="P5313_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRY78953.1"
                     /translation="MEGKPCTDPTPGVAAEIPGCGCSGDPALPECRRTVRPEDLKRAL
                     DARLARIEGQVRGIRRMIAEDAYCDDVLAQVSAARSALERTALAVLEHHMKHCLIDRV
                     RAGQDEIVDELVESLSKLL"
     gene            complement(9012..11504)
                     /locus_tag="P5313_00930"
     CDS             complement(9012..11504)
                     /locus_tag="P5313_00930"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY78954.1"
                     /translation="MKSELIPIRGMTCAACARANERAVGGLPGVAKAEVNFATETLKV
                     DYDESSVGRAAIAAAVADAGYEAVLPPESKTAVVPVGGMTCAACAAAVERSVRALPGV
                     ASADVNLVSEKLTVNYDPEKTRLSEVKKAVRDAGYEPRAVEAETGTDEHAAAKERDYR
                     RLRINFYVAAAVALPLLYIAMGHMVGLPLPKILHPMHYPLNFALVQFVLTLPAIWAGR
                     RFYSVGFRTLLKGSPNMDSLIAIGTSAAIAYSTYSTFEIARGDFSGASHLYFETAAVI
                     IALILLGKGLEAKSKGRASEAIKKLMGLAPTTARVVHGTEEIEIPVDEVEVGDLVRVR
                     PGERVPVDGVVVEGWTSVDESMLTGESLPVEKKAGDTVAGATVNGTGSFVFRATRVGK
                     DTALARIVRLVEEAQGSKAPIAALADKVSGVFVPVVVGIALAAALAWLLAGEGVVFSL
                     RVFIAVLTIACPCALGLATPTAIMVGTGKGAELGVLIKSGTALETAHKVRAVVLDKTG
                     TITVGKPSVTDVEVLGPEGGSGAGDARDRLLALAAGAERGSEHPLASAVIRAAEERGL
                     MVVPPTSLQAVPGRGIEAEAGGRRILVGNARMMGERGVDTAPAAGILESFSREGKTAL
                     FVAADGRLEGILAVADTVKAGSKEAVAAFRSLGIATAMITGDSRAVAEAVAREVGVDR
                     VLAEVLPEDKASEVAKLQAEGYITAMVGDGINDAPALARADVGIAIGSGTDVAAESAD
                     IILARSDLMDAVSALALSRATIRNIKQNLFWAFAYNVLGIPVAAGLLHVFGGPLLNPI
                     FAAAAMSLSSVSVVSNALRLRRFRAPTARAGR"
     gene            complement(11766..13331)
                     /locus_tag="P5313_00935"
     CDS             complement(11766..13331)
                     /locus_tag="P5313_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY78955.1"
                     /translation="MKRARSDGTDGARIVLFVTGMTCGHCERKVAGALEAIPYVDRAR
                     ADARGGRAEAWLRAGTEDGPGIRASLASAVAAAGYEAVPGGAGRRDGLIAAAVGTGLA
                     GLFALAESAGLFSFVPVPDGGSAPGMLLVVGLLTGLHCVSMCGGIALSQGMKGISGPA
                     GDGGRAGALAPSLAYNAGRVVSYTVVGAAAGALGGVLDLGTGGRSIVMAAAGLFMILM
                     ALRLGGILPPSRLRIPLWDRLRERTGAWASRAGSFAVGLANGLMPCGPLQAIQLFALG
                     SGSAAAGALAMFLFSLGTVPVLLAFGAGGAFLPARLRTGAVRAGAVLVAFLGVASLGR
                     AWALSGLDSPLALLRAGSASLPGVRASFGPVAPTAPSAAPSIPSQEAAAPAPIVARRV
                     GGVQVVEFDLSPRRYAEFVVQSGIPVRWVIRAAPGTVNGCNSPLVVPSLGIQKALVVG
                     ENVIEFTPGAPGTVPYSCWMGMIRSRFTVVSDLAAYGPGEAGGPAAAIPADWTPPDLS
                     ARDGDCPTCGNRP"
     gene            13540..14196
                     /locus_tag="P5313_00940"
     CDS             13540..14196
                     /locus_tag="P5313_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HRY78956.1"
                     /translation="MIAPGDRILIGASGGKDSTTLAYDLAVKRRWWPDRFEFLALHVE
                     TDVGEPQDRAPLEALFRDWGVPFASIRVPVIGRLKPGERMNCYWCSTQRRTELIRYAL
                     GKGFNKIALGHHLDDIVETLLMNMLHKGEISTMPPVVRYSKYPLSVIRPLALCEERQI
                     EAFAREKGFASVTCTCGFDGASRRKEVRAKIEALTGGSSELKRNLFRSMSNVNAEYLA
                     "
     gene            14422..15141
                     /locus_tag="P5313_00945"
     CDS             14422..15141
                     /locus_tag="P5313_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HRY78957.1"
                     /translation="MLDLAFSVLLAALPAAILLAWFRRQDRARPEPVGLLGRSVLYGF
                     LATVPAAALELLLLRFGPSGPRLLAAAFDAFVVAGLVEEGVKYFFVRRYLLGRPEFDE
                     RMDGILYAACVSLGFAFAENILYGYADRTVLFFRSFTAVPMHAAVSGILGYWIGRAKI
                     EGGRAAAESASSDRAPRALRGLWEAAFLHGAYDFFLFTRSAWALMAVPVLLAAILRLL
                     FLVADAKRRDRKGLEEEPKLY"
     gene            15203..16009
                     /gene="zupT"
                     /locus_tag="P5313_00950"
     CDS             15203..16009
                     /gene="zupT"
                     /locus_tag="P5313_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRY78958.1"
                     /translation="MEGNIGFALGLTVFAGLSTGVGSLIGFVSKELKPRFLSAALGFS
                     AGVMIYVSLVEIFVKARDSLAIGMGARLGYFVTVASFFAGMLLIAVIDKLLPSYENPH
                     EMNTAAKLEGKSEREKAKLMRMGVFSALAIAIHNFPEGLATFMGALANPTLGVSIAVA
                     IAIHNIPEGLAVSAPIYFATKSKRKAFLLSFLSGFAEPIGALLGYFVLRAVFNESMFG
                     MVFGAVAGIMVYISLDELLPTAEEYGEHHVAITGVILGMVVMAGSLLLFS"
     gene            complement(15995..17998)
                     /locus_tag="P5313_00955"
     CDS             complement(15995..17998)
                     /locus_tag="P5313_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRY78959.1"
                     /translation="MAFVQFTDVSLAFGHRDILKNASLFLAPGTKAALAGPNGAGKTT
                     LLKIAAGLIAPDGGDRAIQKGARISYLPQTGVVYRGCTVYEEAERAYQSVEALVREQE
                     EIGRRLEGSVADDPRTLALVEEHHRLHEAVEGSGYWRRKDRIREVLQGLGFKAGDPDR
                     PVEEFSGGWQMRVALAKVLLENPDILLLDEPTNYLDLEARDWLEDFLRGFPGGFVVVS
                     HDRSFLDSAVTQVYELWNGRLTRYEGTYSAYERTRAQELETLLKDWEKQQEEIAKLED
                     FIRRFRYQASKATLVQSRVKQLERIVPIEIPEGMKRIHFRFPPAPKCGRIALTVEGLS
                     KAYGDKSVVRDLSLTLDSGWKVAFVGRNGAGKSTLMRLVAGVDRDFRGTLRLGSGVTT
                     AYFAQDVAESLDGTRTVEEEALSVCPTDLIPSIRGLLGAFLFRGDDIYKSVRVLSGGE
                     RSRLALLKMLLHPANLLVMDEPTNHLDLDSKDVLLSALKAFEGTVLFVSHDRFFLEEL
                     ADRVLELECGSVPRFYPGGYDYYREKRAALDAEAAAGAGKAAPVRSGPAVSPGKAAPG
                     AGQPAGTGSPRPSGTAQSWEEEKARKAALRRLQKREEEILARLATLGAQKAAAEAEMG
                     KPENYSDGARMKRLQEAAAALDAEAERLNLEWEDVALRLSAEQ"
     gene            complement(18001..19395)
                     /locus_tag="P5313_00960"
     CDS             complement(18001..19395)
                     /locus_tag="P5313_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78960.1"
                     /translation="MSPARRSLVLLAGALLLALSLTGCSRRLGWGVVLWYFEGTEAAP
                     VPEEEATPDGQDTGTGLEAPSPERVPAGTVVPVFIKSNINHTYVVENPATRKKAEVPV
                     WKIELFRRKREAEARAREFEPSARLYAVALRDGLVLRDEPRNLANQVYRMRRDQSIKL
                     LAKTEGEEVTTGGKPLPGDWYLAMGEDGTRGYVFSNQLRIYDESEGPAAGAAVSGPDP
                     AADLDLLFSRTWRPAYFKTMIEQGRVDLERLGPQFGIFTDAVRRQIRIEYPGASRFFN
                     YTNILPQADGSFRFEGLPLTLRFPDPESLEVTIETDYGAGTHPFAVFDQDIREIVRRE
                     ELRRANALARMIAEGERFVSDEWGALTISRSGRFTWAAYDKLIPEGIPEYSGEAGAVV
                     LGLFPGPLLAGEWDGGLSLFFDGGARPRADFVYRATPGGLDLQLVRPIDKEWSVVNTL
                     SEPPLSIRFRRSAN"
     gene            19498..20544
                     /locus_tag="P5313_00965"
     CDS             19498..20544
                     /locus_tag="P5313_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY78961.1"
                     /translation="MAAETGFVAGIDLGATKILAAVFDRGMKVRAQAKVKTPGSGSAD
                     QVLAAILSALDIALAELDLKRSDLEGLGVAVPSPVNRRKGIVLSTPNMGMKDFPLRDR
                     LAGLSGLPVALENDVQAGTLGELRGGALRGKKYAAAFFVGTGIGGGLVLDGRLYRGST
                     GSAGELGHMILQDGGALCGCGRRGCLEALASRTAMARDAVAAAASGKAPILLDHAGTD
                     FRKYRSSAFARSVESGEKAIERIVERSAYWLGVGAANVTAVLNPEAVLLGGGMVARFP
                     DLYKDIAFRSLKDHLMPALGGTVELLITKLGDLAVPLGAAWASRDLAAGRESAAAREV
                     TGAREDMDGQEAAE"
     gene            20541..22079
                     /locus_tag="P5313_00970"
     CDS             20541..22079
                     /locus_tag="P5313_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY78962.1"
                     /translation="MSGQKALAVVEVGSTGIRLIVAAVEASGEYRVLDHAGKPVQLGR
                     DVFVSGSVSRASMLEVIAVLRGFRELLRTYGIEPKDARVIATSALREADNRDTFTDRI
                     ALQTGFSVDIVEDIEENHLMYLAVQKALGEEFRSLARGNALIIEVGGGSTELMLLRRG
                     RMTAAHSLRIGTIRVDEQVRAAMGSPAYLERYLTDNVRTTCDNLDSEMPLATVRNFVV
                     IGSDARFAARAAGTRSESGDYYVVPRESFDRFVGEIQELRVEEIAAKYRVAHGEAEGY
                     SVGLLITKLFLERTAAEELIVPFVSIRDGYLMRITQGPSASLENELHRQVVASTLALG
                     RRYHFDEEHAQHVSELALELFDRLADEHGLGKRERLLLEIAGLLHDVGTFIRTSGHHK
                     HSEYIIANSEIFGLQRDDLTIIANCARYHRKASPSSAHPSFMSLSREDRILVLKLSAL
                     LRVADALDRGHTQRVRIADTERKEDHLVIRVEDGGELTLERLSLEEKGDMFEDVFGIK
                     AVLA"
     gene            22076..24262
                     /gene="ppk1"
                     /locus_tag="P5313_00975"
     CDS             22076..24262
                     /gene="ppk1"
                     /locus_tag="P5313_00975"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRY78963.1"
                     /translation="MIRDRFFNRELSWLEFNDRVLEEGLDPSNPPLERLKFLSIVSSN
                     MDEFFMVRVAGIKAQIRDRDETRDAAGLSPEEQLAAISARVREIQTRQYRCLTEDIFP
                     ALAAEGLCVVRPSDWTGSELRWLEAWFARNILPVLTPLRMDDGEGVPSTGNLRMHAAF
                     LLEPPEVPDGADPAGGAPEGAGGPGGVSGLMGEAPDGRPRLVMVTVPPNLDRFVRIPS
                     ESGRTHLALLDEVILTLGHALFPGYRVLERNLFKVARDAAIGVDEGRDDDFVAAMEEV
                     LANRQNSWPVRMTLGLESPALRERLREVLGLSEEDVYELAGPIDLRSFMELATMKGFD
                     RLRYPARKPVEGYEFSEDNSIFDQIRKGDLLLHVPYESFAPVEEFVRAAADDPNVLAV
                     KMTLYRTSGDSPLVRSLTRAAGKGKQVTVMVELKARFDEAKNIEWASSLEQAGAVVVY
                     GVARLKVHAKACLVVRREEDGSILRYVHLSTGNYNDKTARLYSDLSFFTINETLCREA
                     GIFFNMLTGLSAVEELRTLAMAPFSLKRRIIAMIDREADWSSPETPGLIMAKMNSLCD
                     PDVVAALYRASRKGVRILLNVRGICILVPGVEGLSETVTVVSVVGRFLEHSRIFYFRN
                     GGAEELYLASADWMPRNLERRVELMFPVPDPAARDRVLSILQAYFRDTVKARILLPSG
                     AWERRLPEPGREPFSAQDWFWESERNRFREESEAEERVLTVRRRAP"
     gene            24259..26283
                     /locus_tag="P5313_00980"
     CDS             24259..26283
                     /locus_tag="P5313_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRY78964.1"
                     /translation="MNDPGRPARGPGRGPRGGFRNRGARDAAVVRIVGLPNAGKSALY
                     NALTGAYSVVANYPNTTVDPERTIRREGGVPVRYVDEPGVDGLAPESEDARLLLDRLL
                     SERTDLLLFAADARRLESGLALLWEFLFLGIPTVLVLTGIDEAPVYGVEVEYAALSRL
                     LGVPVAAVSSEEGRGIPELRSRVRTLLSPAGAAGTPRTGGEAASVAPSPPPEPPLPPG
                     IRDARETVRAALAPGVRSPGAAALFLLRADPDLARAYPGAGRTAAVRTALLGLPLERL
                     LSRGRTDWARRIVRSCARIRGRRPGSAADRLAALARHPLWGLPVLGALLLSAYFLVMY
                     ASVDLAGWMDSTLAVPAAAWVADRIASPGIRDFLVGDFGLLTLGVFNALCTVLPILAV
                     FYLFFGIIEDSGYLPNLIVLSRTALGRIGLSGRSVLPVILGFGCKTMATLSARSIPDR
                     KERTIAVFLIAFGIPCSAQLGLNMAILARAGTGAFLIALGFLSAAELGAGAVLNRLLP
                     GESRGGFVLELPPFRVPDPGALLRKTGWRLARFVREALPVFAAAAVALYLADGSGILG
                     AFRDFLAPLVSGWLGLPPSMADALILSMARHEAAAGLILGMAERGLLDRTAMVVSVTV
                     TTLFVPCAANIVAIVKELGKRRGAAVVLAANALAITLGGALNGVLRFLGF"
     gene            26296..26943
                     /locus_tag="P5313_00985"
     CDS             26296..26943
                     /locus_tag="P5313_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRY78965.1"
                     /translation="MTDTRNEDEHLERIWRMEEEGGGSLTLLRTHMGKDYDPLDIESL
                     SRQGCLEVRGDEVAFTDPGRERARRLIRAHRLAELLVRDILGVPDFEAGACELEHLAD
                     TKVVDGICTLLGHPACCPHGLPIPRGDCCRAGAREVTSVVVPLTELEPGKVGRVVFLE
                     ASDEREIRLLGTSQIRPGTELRVRQRRPCYVVDTGTAAVALDEAVAATVKVLPLG"
     gene            27113..29293
                     /locus_tag="P5313_00990"
     CDS             27113..29293
                     /locus_tag="P5313_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRY78966.1"
                     /translation="MRLSAPRPALVLALSLLLAAVLPAQTLAGFDTGLYRIERDGSSR
                     PLWLGGEVKKILATEAGWYFLSSRGVVHSRDLSAFEERNKGLPVKTIKRYDQGVKSFQ
                     YEIQDLKDLEADPYDPRTLVTCTKDAVFLTRDGGLTWTALPAAPSWSTGLKAAAVVSG
                     TENLIFASHPIRGVFVRPLGNPGAAWTEAGGLDRAAGSTSPDEVSDIVAAKGPEGARI
                     WAANSFLPRLYRWDSARRSFAVVHADDRDFAAFESLQVREDGVLYVTDGEIYRYDPRS
                     GRHEAEPGALRAVLAASRAQPGQLESFHGVDSTGKPLQLSELWLAAFKDRKPHRPAAE
                     GRHGLYLQTGFMVNPETRAKYLGVLGRHNLDSVVVDLKDDLGRLRFEPRTELVRRLGR
                     SVNPLDVEAFTAEMKARGVYLIARIVVFKDNHMYKAAGGKYAVWDSAEKAPWRGYFTE
                     KVEAPAPAAPVPSTPPALLAPSRPAPGASVAPAAPPAPAPTYQRKYMEEYWVDPYSEE
                     IWEYNVAIAREIIARGFDEVQFDYIRFPTDGENLGNASYRWRDPGMDMESALMSFLGY
                     ARRNIEAPISIDIYGANGWFRSGVRTGQDVELLARYVDVICPMFYPSHFEQDFLAQPP
                     EELRPYRIYYLGTLRNTFIARKRVVVRPYVQAFFLNVRYDRKWYNPDYVRRQVEGVRD
                     SANLGLTFWNNIGRYDDIPVLDVRGNRRLAAGPEALRPKGVQLD"
     gene            29372..30586
                     /locus_tag="P5313_00995"
     CDS             29372..30586
                     /locus_tag="P5313_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY78967.1"
                     /translation="MKPSQPFRVRFLAFAAAALAALLLFRLMHAPGTLGLLVWNPSYR
                     SFAYGILALGAVPFLLAIVYEVLRGIPSFRRRRAAGLVSMGVSAVSGAAALAAILFLA
                     IAPNRVPDPLPTPRILDPSRGIVVRPSGDGGPYLRLAFSSDPHWGREQSDEAARTRIL
                     TSVDAGDYDAFFLLGDISEQGFPESGFREAALDLETYLTRVPVRPMMGNHDALVNAAG
                     RWARYFFPSGHSSDSGSPLYYRIDAGRAHILVLNLLWGEEDFDHAQRAWLTEQLESIP
                     RQDAVVVLSHCYFYASGYDDPESGSPWYDHPGTIREVVPLLETYGVDLAVSGHNHYQE
                     LLEHNGVTYAVIGAMGGVPDPAPTYRSPASRWIRVSTFGFLEARFYPDRIELAFRDAD
                     GGELHRAVVSAE"
     gene            30597..31748
                     /locus_tag="P5313_01000"
     CDS             30597..31748
                     /locus_tag="P5313_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY78968.1"
                     /translation="MESVADPAGSGSARFQEASFTALDRYRGNPAAPLRFLLFRGGLP
                     DLARRGQELAFPGLPYADAVSANAVSGIPRPGESGPVDGALPDPVSVPCRYVCAEADE
                     DPEPHAYGLLDLSYDPETRRYRHPRQRFPDPREPVIYPCDAPDDLTLFETAVLLARYP
                     YRLPEGGFVVDVPRNLSPMFQRDLLTLILTGRDPGSALEWLGRTGFLDAYWPELAALR
                     GVSHSKEYHPEGDVWAHTLETFRYRKLSEFRLSLGLLLHDTGKPESRSAEGRRFDQHS
                     EIGARLARRFLERLGFARDLSEDVEFLVRRHMMPAALPRLPLNRVQEDLEHPLFPILL
                     ELYRCDELSTFRGPDGYYQACAAYRQYLRNVRNPYRNPDGRKVQRMFLE"
     gene            31757..31978
                     /locus_tag="P5313_01005"
     CDS             31757..31978
                     /locus_tag="P5313_01005"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRY78969.1"
                     /translation="MKEAYRCVYMEDALVVKSLLDSAGIPSRILSGDMLDVNPFFSAT
                     VQGVRILVSDEDWEDARAVAADFKSRRED"
     gene            complement(32183..32923)
                     /locus_tag="P5313_01010"
     CDS             complement(32183..32923)
                     /locus_tag="P5313_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78970.1"
                     /translation="MDRRRYEKFTALRDAFREKVRLWTAAVPELRDIQERLRIARGYD
                     DYVVETPVVYNRALDDIGPESRPRWILIADNPGKQEQKSANLRYLVGQSGKLAEGFFR
                     RELDTDFRRDVLIVNKTPVHTPKTAELALLRNLDASGAVEALLAESQAWMALFARALH
                     EALGVPVWISGRSELKPKGIFGSWFREYSRLYREAPAAVREEVLVFSHFSMNQFSLEL
                     SRKRDPGRPLMEELRRIGAENRRAIFNF"
     gene            33020..33799
                     /locus_tag="P5313_01015"
     CDS             33020..33799
                     /locus_tag="P5313_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78971.1"
                     /translation="MKSTRRLMIALVLLLGAHAAFGQGRFRNILSLKFEYFTDQRFGL
                     EWEEVFLAPLPKGFYLVSAVSGTNTSFSDLTGGRLGLAFDLPGSFYGEFSYEFEYDWR
                     VAQPKHTPLVSCTYESGPAMASLSLSGEFTPDSAGGVLSPSLKYTLNPRFFLSSSLFL
                     AFHHYSTSESFFNFAALATGEYGLGPEIFLSLGGTFGTVYEPDARYEKWSVLGGVKVV
                     PSNSVAVKCLVEFENADAAPVNPHNIISAQIVVDVKFRAKE"
     gene            33861..34193
                     /locus_tag="P5313_01020"
     CDS             33861..34193
                     /locus_tag="P5313_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY78972.1"
                     /translation="MGIETIGAALVLTPEIKHLDILSSKEFAERFAPAAKDARTVILD
                     LSQVEFVDSSGLGALLNLVRDTLGRGGRISICRPQPSVKVLFRMVQLSKLVSIFDGKD
                     AALAWAET"
     gene            34190..35875
                     /locus_tag="P5313_01025"
     CDS             34190..35875
                     /locus_tag="P5313_01025"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY78973.1"
                     /translation="MSGVPPDLIPWVPAAAVSVSLCALAAAAVLAARRGPLLPLDRPE
                     YREFAASLDGAVALISGDLEKVLYASPGFEPLFGSTPDRVSFRRLEGVVHAEDLEALE
                     SALSAGRMRPFEVEFRIRSEGEPRWLRMRGFPVAGTGRKAARRIAVWVEDRSLRKTDE
                     LRLVEALDYEVAVGARIQQALLLGSPERAYPGFDLASMTLPSQKIDGDFMDFFDGGPE
                     VLDLMLGDVMGKGVPAALTGAAACNAFFRARLSLAEQAGESLPPVKDIVRGAEERIAG
                     KLMDLRTFFTLVYGRLDGKRGLYRYVDCGHTSVLHFERKTGRCWRLKGANAPVGFLPE
                     QDYVEYAVPLSPGDLLFLYSDGITEAENSEGERFGEERLMKLVGSHSALDAAALLDKV
                     KHIAFAYAGGDFKDDVTALAVRVASGPSVRTARRSFPQSMDSLRAVREFYRACADRAL
                     SASLPSEDLDALLIATGEAAANIFRHSVPDRPADCTAAFRVSDSWAAFYLYYRGADYE
                     WQEPTAPPIESYPAGGYGLYLINEAMDSVTVSPGEDGEVRLCMLVDLASRARP"
     gene            35872..37872
                     /locus_tag="P5313_01030"
     CDS             35872..37872
                     /locus_tag="P5313_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY78974.1"
                     /translation="MRGIREFGDGIYRIAAAGEPRLFPGYSYLVTDAGRGVLLDPGPA
                     SDFAATLALCRSVISPERISFLILTSETPDAASSLPLWPASGFSGRVLAHWKAGLQAR
                     CYGGPEIRDLGNHEERISFGGGRTFRFVNVPGLPSAGSIFCLDEASGSLFTGMALGSV
                     GGGETEGDEESPGRIESFHDLVFPALGARPDVLAALASLSPRRILPRNGPPVERHLSV
                     ASVLFGCGDGAACGPGDLPEACARLIGEVNQRLADVFSPAELRDVLAETGLGFGADGR
                     PEPADLAPSDLLERYFDTLARVKGPVWISLVRAHVARGTEALGIPLPAAFDRFGREID
                     RGIGGLIRDIERIRTENIELQKSIIQASDDHLKDPVTGYYNEIFFKEYLDDLLLAEAG
                     AGTPPGSVAFIRLDGIRKLNERFGTEAGDTALKALGLHLLDTKRPNSIFFRMNGPLFA
                     CYLQGDGKTQAVDYVRELQAEVEGSGRFVEKVTVSAAIVELSEISTVAAETGKPYACL
                     MKVCKERIHLLDKLGPASICHESSVTLRRNRGNVLVIEASPFEADLLRKVLEREGFEV
                     HSVTRGGEALAHADLYRPDVIVSEIFVSQMDGFQIRQRLLASPDLKNIPFILVSWDKG
                     ASALRQAFDLGILHFYKKPVQPEELIGIIKLLAGEASEERRP"
     gene            37869..>40241
                     /locus_tag="P5313_01035"
     CDS             37869..>40241
                     /locus_tag="P5313_01035"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY78975.1"
                     /translation="MILPISLLLFFLAANGVTVLALLVVKRRGRVIYRRAQTVRGALI
                     SAVIDRDRSGLGKLARRNSGLSIRLWADLRATFYFTDREAEPVLEVFRSSGIEARLLR
                     RLRSREPLERFRASRMLLPLVSPGIVPALVRALHRETRDFVRIALVKDLLSSDLSPAY
                     DAIAESLRDCGMGYFRSVRAMLAAVGNGFVDWARDRLVPSDPGFAVAVLAGARTHPAP
                     WLRDFVLICLDHDDPRIRSSAIEAAESLYPEMLAGRAEDRGADIVTRRASLRAQAEVS
                     YPLDLEAFAEYLTDPTLRDVAASCLSEYLGRYPEEAGRILELLSRLPDSEAARALAGV
                     LGVRLPFLFVSGEDPGRCRRVVEGLAASGRSSEIIALLNSNRDSELEGLLLEWIRPYL
                     GTVPEFRRDCAGYLEPRLREALGIAPEEVPKSSRKIPLTRRDRLRLVVVLAAVVAVPS
                     AAIAYGLSGATIGRTWILAREIFRLFTWGFAFYAVFLNLVYIVLLLLAARNLGIQAKY
                     WAVLDKDFLFTPGIMPSISILAPAYNEEKTVLESVHSLLSLQHPDYQVIVINDGSSDD
                     TMGVLARAFDLERMDPSRTGSLPTAPVRGVYRSPGNPKLLVVDKENGGKADALNAGIN
                     LARCEYICSIDSDSLLEPDALLRMTAEVLTSDVETVAVGGNILPVNGCTVERGMLQKI
                     GLPSNRIARFQTIEYLRSFIAGRLGWAQINALLIISGAFGLFRRDRVLEIGGYLTGRG
                     EFKRDTVGEDMELVVRLVRRMRESRRKYRIRYAATANCWTDSMPTWPCSGR"
BASE COUNT         5794 a        14096 c        13995 g         6356 t
ORIGIN      
        1 gccgatgtat ccgccctggt gagccacgaa gtacttggtc gcggtctgcc cgaacgccgt
       61 ccaggcgcag agcaggagcg cgatggtgat gattgcgatc ctcttcatgg gatcctcctt
      121 caaagtgccc gccttgaatg cagaaagggg gctccgcctc cgggttcaag gcctcgggga
      181 ggttagtacc aatatgaatt ctccaaatga ccgatttagt caagatgaaa atatagattg
      241 tagattatag gtaaaaaaat atataaacag ggctgcctct ttgtacatct gcggcagtgg
      301 cgtggcgccg gattccggtg tatagtccct ggaaatgttg agatcatcct tccggaaggc
      361 gttccaggcc cgctcccggg aagcggtcct cctgctggtc cgcatgcccc tgaccgaggt
      421 caaccggccc gccctggcag ccgccgcgcg ccgagcctcc gtggaagccc tcctggccgt
      481 ccggttcccc ctggtccggc tctgccacgg tatctccgtg cttgcctccg agacctccgg
      541 ccccggcata cccgagggag ccacccgcat ccgcgcactc cgggccgcgc tgaacgcggc
      601 ggaggccgaa gccgggctcg ccgaaggctc cggtgccacg gcctccgata caccggggat
      661 gagtctttcc ggctgtccct gctcccgggt cgtgcccgtc cggggtcggc ttcccctcag
      721 ttcgtccctg atccgggccg ctgtttcgtc cggagcggga ctgcggatcc tccggttccg
      781 gccctcgggg ctgaagggaa cgccccagga cctctcggac cgcctttcgg acctccttcg
      841 ccgggaggcc ctcctggggc tctacgcgat cgccggcaga cggcccgatt ccccggcccg
      901 ggaattcgcc ctgttcgtgc cggaccggcc gccggaggcg tccggcgggg ggagggggtc
      961 cgatcctctg gcgggattcc cggtgaccgg acgcatccgg accctggtgg acgcgggggc
     1021 ggaggccccc tcggacctgg cgggcttcga gctcgccgac ggggatcccg gaacgaagcc
     1081 gtcgggaccc gccccgatcg gaaaggtcga agggccgccc gcggagaagc ggcccgcaaa
     1141 gtcggccgcc ccggtccgga gggaagcccg ggtggcggag taccggaacg cggcggtcct
     1201 gcgggcggaa attccggacc tcctcggtgc cctcaagggc atggaccccc gggaggcggc
     1261 ggcctacctg aacagggtcc tggcccggtt ggcatcctgc gtggaagccg ccggggggat
     1321 cgtggactcc tgcgcgggcg ggggaatccg tgcgttctgg ggggctccgg cttccacggg
     1381 ccgggatgcc gaggctgccg ggaaggcggc cctcgccatg cgccggacga tcggggccct
     1441 gaaccggggc cgcttggcct cggggaaggt cccgctccgg cttttctgcg ccctggacgc
     1501 cggccccgtg ctggccgccc cgatcggagc cccggaccgc ccggcctaca cggccgccgg
     1561 ggaggcgccg cggagagccg ccggcatggg gcaccggaac ggccgcctgg gaacggacat
     1621 cctggtctcc gagtacgccg tgcgcctcat gggtgcggcc ttccggttcc agcccgtgga
     1681 caggctggaa gccggggggg aagccgggac ggtgggtatc tacgccctcc tgggccgttc
     1741 gggggacgcc tcgggccccc gggatctagg ggacctgagg agacggctcg ggacggtcta
     1801 ggagagcgtc ggcctcctag gagtttgtcg ggcttgccca cctaaaaagt agaaaaatcg
     1861 catttgtgcg atttttctac cttgcaaact ccaaaggagc cgcataatcg ggatttttag
     1921 cgtctatcgg cactaaaaat ctacaagtcc gactggctcc tagaacggct cgaaacgctg
     1981 ggcgtcgaac cagtagcgct cggcctcgtc ctggatggcc aggaggtcct tgtagtgctg
     2041 ctgttcccag tccgccaggg tgtcgaagaa ggtcctcagg gcgggaacca cggtttcctc
     2101 cgcggccttg cggtaatgcc ggaccgcctc cacctccaga aggacacctg ccgccacggc
     2161 ggtcaccagg tgctggctcg aacctacccg cttcaggaac tcttccgtca ccagggggct
     2221 gcgcccctcc atgccgaaga cttcggcggc caggttctcg ctgggcatcc tgccctggga
     2281 aagttccttg taataggaga ggagccagtt gtagtgccgc tgttcctcct tcccccgctc
     2341 ccggaagaac tcggccaccg gaccgtcgga tttctccgcg gcctccatgt agatggtcac
     2401 gctgtccagt tcgcccttca ggcccttttt aacggccgcc aataagtgtt ccatcccatc
     2461 aatatacttc cgcggacccg ggttctcaag ggcgcgggct tgccattgcc gcccttcggt
     2521 ggtagcgtcg ccgcatgagc atcgagcagt tcgcccaggg ggtggccttc ctggcctccc
     2581 ggtccccggt tttcggcgaa aagccccttt ccgtgaccct gatcgcgaat ccccgggcgg
     2641 gggggttcac ccgtcccttc gtattctccc gccacctggg ggagctcaag ctcctggccc
     2701 ggcgggccgc cgaccttccc gtccgcggcg cccccacgac cttccggctg cacgccacgg
     2761 cgtatccccg gcacgccgcc gaggtgaccc gggcactggc ggaggaggcc cgggcggctc
     2821 cggagacgga gcgcctcgtg gtcaccgcgg gaggggacgg cacgaccctg gaggccctct
     2881 cgaccctcat ggacctgagc gaggcggagc ggagccgctt cgtggtcctg cgcctgccca
     2941 tgggcaccgg gaacgacggg gcggattccc gggaactgct gagcgccctg gagctcctcc
     3001 tgggtccctc ccggttcgac cgccagagcg ccctccgggt catccccgcc tcggccggta
     3061 agcccggcgg cagggggccc tggtggtcct tcaatatcgc ttccctgggg gcggacgcct
     3121 tcgtctcgga catgaccaac cgcctcaagc tgaccttccc gggagactcc tacaagctct
     3181 gggtggagct ggccacggtc ttctacgacg ccctctggcc cacccggggc ttcgaggtcc
     3241 gggcctggga cgcgaagggg cgggaagtca agagcttccg gggtgacctg ctccttctgg
     3301 ccatgggggt gtcgggccga aggacctacg ggtccaacaa gcccatcctg ccggacgacg
     3361 acgacgtctg cctggtgcgg cagatgcccc tcctgcggaa gctcgtcgtg aagggccccc
     3421 tgacctcggg acgccaccgg ggctacccgg aggtggacct cttttcggcc tccaaggtcc
     3481 agatcggctt ccgtcaccgc gccctggtcc agatggacgg ggaggcggag cgccttgaag
     3541 cggaggattt tcccgtggtc ttcgagcgca ccgagcccgc gatccgggtg ctccgcaggg
     3601 cctgagcggt tccgcccctc ccccggcagg ccgatccggc ctcggccgcg gcgggctcct
     3661 agaagatcga catccccgtc ttggtggtga agagctccag ggcttgggtg cccgccaggg
     3721 aatttccccg ctcgttcagg cccggggacc agacggcggc ggccaggcgg cccgggagga
     3781 tcgccgcgat ccccccgccc accccgctct tggcgggcag gcccaccctg tagacgaagt
     3841 cccccacggc gtcgtaggtc ccgcaggtga gcatgagggc cgcggctcgc ttggccgggc
     3901 tgggctccag gacccgctcc ccggatacgg ggcagaggcc gcggttcgcc aggaagagga
     3961 agctcgcggc caggtcccgg cagctcatcc ggagggagca ctggaagaag tagaagtcca
     4021 ggacctcctc cacccggttc cgcaggttcc cgaagctctt gagcaggtgg gccagggcgg
     4081 cattccggtg ccccgtggac cgctcggact cggccacctc ggggtccgcc cgtacggctc
     4141 cggaccccga acgggcgcgc acgaagcgga gcagttcctc ccggggatcc tcgagggcct
     4201 ccagaaggat gtccgccacg accagggccc cggcgttgat gaaggggttc cgggggatgc
     4261 cccgttcctg ctccaggagg atcggggaat tgaaggggtc ccccgagggc tcccgttgga
     4321 tgcggtccca gagcccgtcc ccggcggagc gcagggccag gatgaaggtg aagaccttgg
     4381 atacgctctg gatcgagaag ggctcgtccg cgtccccgac cgcgtggatc cggccctcca
     4441 gtgtgcgcac ggccatgccg aagcggtccc gggggacccg ggcgagggcg gggatgtagt
     4501 ccgccggcgc gccgagggcc aggagggggc ggacctcctg cgcgatgtct gcgaggatcc
     4561 ggtcgtagtc gggttccatc gggaaattgt atccgccgcc gcgggggatg ccaaccacgt
     4621 cgtggccgtc gccccgcccg ccgtggtatc atctccccat gatccttccc gaaaatccct
     4681 ccgccggccc cttcccggag ctggcgatcg acctggatgt cctttcctcc aacgtccgtt
     4741 ccctggccgg cctttgcagc gcccgggggg tctccctgac cggggtcgta aagggggcgg
     4801 acggcctggc ccccgtggcc cgggcgatgc tcacgggggg ctgcgaacac gtcgcttcct
     4861 cccggatcgg ccagctccgg gccctgaggg aggccggaat cgaggcgccc ctcctcctgg
     4921 tccgcgtccc cgccccgggg gagctggcgg agacggcccg ctgggcggac cggagcctcc
     4981 actcggatct gtccgtgctc cgtgccctgg accgggaggc ggaccgggcc gggcgcccgc
     5041 acggggtcgt cctgatgctg gatctggggg acctccggga gggctggtac gaggaggggg
     5101 acctcgtcga ggcggcggtc cggaccgagc gggacctttc cgccctggag ctcctgggga
     5161 tcgggaccaa cctgggctgc tacgggtcga tcctgccgac cccggagaac ctgggccgcc
     5221 tggcccgggc cgctggccgg atcgaggaac ggatcggccg ggccctcgag gtcgtgtccg
     5281 ggggggccac ctcctccatc cccctcctgc tgtccggcgc cctgcccgcg gggatcaaca
     5341 acctccgggt gggggagggg ctcctgacgg cccgggacct gccgcacttc tacaaggtgg
     5401 acgtgccggg gctgagcccc ggagtcgtca cccttcgggc ggaggtgatc gaggtccgga
     5461 ccaagccctc ccacccggtg ggtgagatct tcatcgacgc attcggggag cggccgacct
     5521 acgtggaccg ggggccccgg cgcagggccc tcctggcggt cggcaagcag gacttcggga
     5581 agcacgagtc cctggtgccc ctggagcccg gggtcgaggt gatcgggagc tccagtgacc
     5641 acctgatctg cgacgtcgag gacctgggcc ggcccctggc cgcgggggat atcctggagt
     5701 tcggcctatt ctacccggcc ctgctcttcg cctcccagag ccgctacgtc cggaagacct
     5761 tccggggcgg cgggcaggac tagcaggaag cggctgcccc ggccgggccc cgggctctcc
     5821 gcgtaggcct cccccccgtg cgcccgcgcg gctgcccgga cgatggccag ccccaggccg
     5881 aagcccccgg tatcccggga ccgggaggga tccgcccgga agaaccgctc gaagacccgg
     5941 ggcaggtcct ccggggagat cccgatgccc tcgtcctcca cctccaccac ggcccaggcc
     6001 cgggttcccg gcgcgggggc cggctccgga aggtccccgt cgccgcgcgc gaggcggacc
     6061 cggacctcca cggtcccgcc gggatccgag aaccgcaggg cgttggacag gaggttgccc
     6121 acggcgccct cgagggccgg gcggtccccg gacaccggga cgggctccgg ggcgtccgtc
     6181 cggatgtcca ggttcccggc ccgggcggaa tcccggtagg ccgcgcagac cgaccggacc
     6241 gcggcggcaa ggtcgaaggt ctccggggcc ccggagtacg ccgcggcgtc ggcccgggcc
     6301 aggccttcca ggcttcccac caggcttccc agccggaggg cgtcccggcg gcaggcttcc
     6361 agggccgcct cgtcgggagt ccggaccccg tccagcaggg cttccaggcg ggcccggagg
     6421 gaggccaggg gggtgcggag ctcgtgggac gcgtcggcca ccagccgctt gcggagctcc
     6481 tcctgctccc ccagggctcc ggagaggcgg gagagggcgc cggacagctc gtcgagctcc
     6541 cggatcccgg agggcggaag ccggatgtcg aagtcgcccc tgcctatcga ccgggccgcg
     6601 cgggcggccg agcggagggg agcggaaagc cgccgggcgc tccaggcccc cgcaaggacg
     6661 gccagggctg ccaggcccag gccgaccgcg gccaggaccc ggtccaggga gcggaggaag
     6721 gccagctcgg cgtcccggta gtagacgggg ccgtagtatc ccgccaccag ggcggggggg
     6781 gccgcgtctc cgggcaggga ggggagggcg acggaccgtt cccggtagcc tccaccgcgg
     6841 tcggctccgg ccatgcggac cgccatggac tcgatcatct ggctgcagag cccggcgttg
     6901 tgttcccggg cgtcccagag gaccgacccg gttccgtcca ggaggcgcag gatgaagccg
     6961 tcctccaggg cgcccacccc cagggattcc atgcggccgt ggtcccaccc gcccccggga
     7021 tcccgggcgg ccgagacgga ggccgcgagg gcgtgcacgc gggactcgat accggcggct
     7081 atgtaggcgt cgaagcgttc ccgggccacg agccgggccg ccccgcccgc ggccaggacg
     7141 gccgccgcgg ccaggagggc gtgggagagg gtgaggcgac ccctcagggt ggtgttcaag
     7201 gttccctccg gaagcggtat cccatgcccc gcaccgtctc gatccagcgg gggttctcgg
     7261 aatcctcatc gagcttgcga cggaggttct tgacgtgggc gtcgatggtc cgctcgtatc
     7321 cgtcccaaag ctcccccagg gcccgggcgg cgatctcctc ccgggagaag acccggcccg
     7381 gccgcagggc cagggcctcc aggatcctcc actccgaagc ggtcagttcg acgcaggctc
     7441 cgtcccggca ggcggtccta ccttcccggt ccagggagag ccccgggaac tccagccgcg
     7501 accccggcgg ccgggccctc cggagcaccg cccggacccg ggccgcgagc tcccgggggc
     7561 tgaagggctt ctccatgtag tcgtccgccc ccaggtcgag cccctcggcc gcggcctccg
     7621 ggccggaccg ggcggtgagc atcagcacgg gcacggcgga ttccgcccgg aggcggcggc
     7681 agacctcccg gccggacagg cccggcagca tcaggtccag gacgaccagg gcgtactccc
     7741 ccgtctccgc ccgggccagg gccgtcggcc cgtccgcggc ctcgtccacc gcgaagccgt
     7801 cccgttccag gtaggcccgg acagtctccc ggatgccggg ctcgtcgtcc acgaccagga
     7861 cgcggtccac ggcgcctacc tcaggtcata gggaagaaga agcttctcga cctcgaccat
     7921 cttgccgtcc ggcccgtcca cggggacctt ggcgagggtc caaccccgga cccgggaagg
     7981 gtccgcgccg gcggcggcca ggaaggcggg atccaggatc aggacccagt ctttgtcgtt
     8041 cttggcgggc tccgcggccc actcgaccag gttccccccc cccagggaca gcccgtagtg
     8101 gcccagctgg gcgtggtagc cgatccggtc ccgggccgag gcggccaggg cggccatgtg
     8161 gtccgccgga gagccgcccg gtttgccgcc cagaggggag gctcccaggt tgaagcggcc
     8221 ggtcagccgg ccgccctcca cggtccagga caggtccggg ccggagggaa gccgcgcccc
     8281 gtccagcccg gcctccagga agggcaccgc gtccagggat aggaccgccc gcggcgggga
     8341 tccggggtcc gcggacaggc gcagctcctc cccgcccggg gaggcgagga cgcggtactc
     8401 gcccggggac ggggagccga gggcgccggc cgcggtcccg aaggaccggg cggcgtactt
     8461 ggccacgacg tccgtggatc cgcaggaaac ggccaggaag gcggccgcca ggacggccag
     8521 gatgagcgag gggaaggaag gggcgccgag gtgcccggga tgccgggaaa cgggattcat
     8581 gggattcctc cgcgggcggc tctctcgccg cccggattcg ggttcgtttc tgttcgaacc
     8641 gctacaggag tttggacagg gactccacca gttcgtccac gatctcgtcc tgaccggccc
     8701 gtacccggtc gatcaggcag tgcttcatgt ggtgctccag gaccgccagg gccgtgcgct
     8761 ccagggcgct ccgggccgcg gagacctggg ccagtacgtc gtcgcagtag gcgtcctcgg
     8821 cgatcatgcg ccggatcccc cggacctggc cctcgatgcg ggccagccgg gcgtccaggg
     8881 cccgtttgag gtcctcgggc cggacggtcc ggcggcattc gggcagggcg gggtctccgg
     8941 agcacccgca gcccgggatc tcggccgcca cgccgggcgt gggatccgta cagggctttc
     9001 cttccatagg tctacctccc cgcccgcgcc gtgggagcgc ggaagcggcg cagtcgcagg
     9061 gcgttggaca ccaccgagac cgaggacagg gacatggccg cggcggcgaa gatcggattc
     9121 aagaggggac ctccgaagac gtggaggagt cccgccgcca ccgggatgcc caggacgttg
     9181 taggcgaagg cccagaagag gttctgcttg atgttccgga tcgtcgcccg ggacagggcc
     9241 agggccgaca ccgcgtccat gaggtcgctc cgggccagga tgatgtccgc ggactccgcg
     9301 gccacgtccg tgccggagcc gatggcgatc cccacgtccg cccgggccag ggccggggcg
     9361 tcgttgatcc cgtccccgac catggcggtt atgtagccct ccgcctggag cttggcgacc
     9421 tccgaggcct tgtcctccgg gaggacctcc gccagcaccc ggtccacccc gacctcccgg
     9481 gcgaccgcct cggccacggc ccgggagtct ccggtgatca tggcggtggc gatgcccagg
     9541 ctccggaagg ccgcgacggc ctccttgctc cctgccttga ccgtatccgc caccgccagg
     9601 atgccttcta gcctgccgtc cgcggccacg aagagggccg tcttgccttc ccgggagaag
     9661 ctctccagga ttccggcggc gggggccgtg tcgactcccc gctcccccat catccgggcg
     9721 ttgcccacca ggatccggcg gccccccgcc tcggcctcga tcccccgtcc cgggacggcc
     9781 tgcagggagg tcgggggaac caccatcagt ccccgctctt cggcggcccg gatcaccgcg
     9841 gaggccaggg gatgctcgga accgcgctcg gccccggccg ccagggccag gaggcggtcc
     9901 cgggcatccc cggcgccgga tccgccctcg ggacccagga cctcgacgtc cgtgaccgag
     9961 ggcttgccga cggtgatggt gccggtcttg tccaggacaa cggcccggac cttgtgcgcc
    10021 gtctccaggg ccgtcccgct cttgatcagg acccccagct ccgccccctt gcccgtgccg
    10081 accatgatgg ccgtcggggt ggccaggccc agggcgcagg ggcaggcgat ggtgaggacg
    10141 gcgatgaaga cccgtaggga gaaaacgaca ccctcgcccg ccaggagcca ggccagggcc
    10201 gcggccaggg cgatgcccac gaccacgggc acgaagaccc cggagacctt gtccgccagg
    10261 gccgcgatgg gggccttgga gccctgggcc tcctcgacca ggcgcacgat tcgggccagg
    10321 gccgtgtcct tgcccacccg ggtcgcccgg aagacgaagg accccgtgcc gttgaccgtg
    10381 gcccccgcca cggtgtctcc ggccttcttc tcgaccggca ggctctcccc ggtcagcatg
    10441 gactcgtcca cggaggtcca gccctcgacc accaccccgt ccacggggac gcgctccccg
    10501 ggacggaccc ggaccaggtc gccgacctcc acctcgtcca cggggatctc gatctcctcc
    10561 gtcccgtgca cgacccgggc ggtggtgggg gccaggccca tgagcttctt gatggcctcg
    10621 gaagcccggc ccttggactt ggcctccagt cccttgccca gcaggatcag ggcgatgatg
    10681 accgcggcgg tctcgaagta gaggtgggag gctcccgaga aatcgccccg ggcgatctcg
    10741 aaggtggagt aggtgctgta ggcgatcgcg gcggaggttc cgatggcgat caggctgtcc
    10801 atgttcgggg aacccttgag cagggtccgg aagcccacgg agtagaagcg ccgtcccgcc
    10861 cagatcgcgg gcagggtcag gacgaactgg accagggcga agttgagagg atagtgcatg
    10921 gggtgaagga tcttcggcag gggaaggccg accatgtgtc ccatggcgat gtacaggagg
    10981 ggcagggcga cggcggcggc gacgtagaaa ttaatccgca gcctccggta gtcccgttcc
    11041 ttggccgccg cgtgctcgtc cgtcccggtc tcggcctcca cggctcgggg ttcgtacccg
    11101 gcgtcccgga cggccttctt gacctccgaa agccgggtct tctccgggtc gtagttgacc
    11161 gtgagttttt cggacaccag gtttacgtcc gcggaggcca cgccgggcag ggcccggacg
    11221 gaccgctcca ccgcggcggc gcaggccgcg caggtcatgc ccccgacggg cacgacggcg
    11281 gtcttggact ccggcggcag gaccgcctcg tagcccgcgt ccgccaccgc ggccgcgatg
    11341 gccgcccggc ccacggagga ttcgtcgtag tcgaccttca gggtctccgt cgcgaagttc
    11401 acctccgcct tggccacccc cgggaggcct cccacggccc gctcgttggc ccgggcgcag
    11461 gcggcgcagg tcatgccccg tatcggaatc agttcggact tcatgctggt ggttctcctc
    11521 ggggcggctc gacggcccgc ccgggtctcc tatatccccg acggattcgg tcttggaatg
    11581 gatgctcagc cggaagagaa gggtctcaag ccttccatag tccattccac gggaataact
    11641 catcctaggc agccggtttg gaaatcccga cgagggattt ccaaaccgat tcgtcgcgaa
    11701 tcggacatgg tccgattcgc gacgataccc cctgggggta tattaccgct gccggggacc
    11761 gggggtcaag gacgatttcc gcaggtggga cagtccccgt cccgggcgga gaggtccggg
    11821 ggggtccagt ccgcggggat ggccgcggcc gggccgcccg cctccccggg gccgtaggcg
    11881 gccaggtccg agaccacggt gaaccgcgac cggatcatcc ccatccagca ggagtagggc
    11941 accgtgccgg gcgcgccggg ggtgaactcg attacgttct ccccgacgac cagggccttc
    12001 tggatgccca gggagggcac caccaggggg ctgttgcagc cgttgaccgt gcccggggcg
    12061 gcccggatca cccagcggac cggaatcccg gactgcacca cgaactcagc gtacctccgg
    12121 ggagagaggt cgaactcaac gacctggacg cccccgaccc tgcgggcgac gatcggggcg
    12181 ggagcggcgg cctcctggga ggggattgag ggggctgccg acggggccgt cggagcgacg
    12241 gggccgaagg aggcccggac gccgggaagc gaggccgatc cggcgcgcag gagcgcgagg
    12301 ggggaatcca ggcccgaaag ggcccaggcc cgtcccaggg aggcgacgcc caggaaggcc
    12361 accaggaccg cgcccgcccg gaccgccccc gtccggagcc gggccggcag gaaggctccc
    12421 ccggccccga aggccagcag cacgggcaca gtgcccaggg agaagaggaa catcgcaagg
    12481 gcgcccgccg cggcgcttcc ggagcccagg gcgaagagct ggatggcttg cagggggccg
    12541 cagggcatga ggccgttggc caggcccacc gcgaaggaac cggcccggga ggcccaggcg
    12601 ccggttcgct cccggagccg gtcccagagg ggtatccgga ggcgtgacgg cggcaggatg
    12661 ccgcccaggc gcagggccat gaggatcatg aagagccccg cggcggccat gacgatcgag
    12721 cgcccgccgg ttcccaggtc caggacgccc cccagggccc ccgccgccgc gcccacgacg
    12781 gtgtaggaga ccacccgccc ggcgttgtag gccagggagg gggccagggc cccggcccgt
    12841 cccccgtcgc ccgcggggcc ggagatcccc ttcatcccct gggacagggc gatcccgccg
    12901 cacatggaga cgcagtgcag gcccgtgagc aggccgacca cgaggagcat ccccggcgcg
    12961 gaaccgccgt ccgggacggg gacgaaggag aagagtccgg cggactcggc cagggcgaag
    13021 agccccgcga ggcccgtgcc gaccgcggcc gcgatgagcc cgtcccgacg gcccgcgccc
    13081 ccgggaaccg cctcgtagcc cgccgccgcg accgcggagg ccagggaggc ccggatgccg
    13141 ggtccgtcct cggtcccggc ccggagccag gcctcggccc ggccccctcg ggcgtccgcc
    13201 cgggcccggt ccacgtacgg gatcgcctcc agggccccgg cgaccttgcg ctcgcagtgc
    13261 ccgcaggtca tccccgtcac gaaaagcacg atccgtgcgc cgtcggtccc gtccgatctc
    13321 gctcgcttca tgtctcctcc ccgatcaagc ataccggagt ctaggccggt attgtgaagt
    13381 tcccgtgaag gagcgggatc tcgtccacca ttcaccggtc cgtccgagga ttgcgggggc
    13441 gggctggacg cgggtgcggc cggccggtag gatgcgtccg tgaagaacgc ggaaaccacg
    13501 gtggccaagc tctccgaggt ggccctccgg cgctggtcca tgatcgcccc gggggaccgg
    13561 atcctcatcg gggcctcggg gggcaaggac tccacgaccc tggcctacga cctggcggtc
    13621 aagcgccgct ggtggccgga ccgcttcgag ttcctggccc tccacgtgga gaccgacgtc
    13681 ggggaacccc aggaccgggc ccccctggaa gccctgttcc gggactgggg ggtgcccttc
    13741 gcgtccatcc gggtcccggt gatcggccgg ctgaaacccg gggagcggat gaactgctac
    13801 tggtgctcca cccagcgccg cacggagctc atccgctacg ccctgggcaa gggcttcaac
    13861 aagatcgccc tgggccacca cctggacgac atcgtggaga ccctcctcat gaacatgctc
    13921 cacaagggcg agatctccac catgccgccc gtggtccggt actccaagta ccccctgtcg
    13981 gtgatccgtc ccctggccct ttgcgaggag aggcagatcg aggccttcgc ccgggagaag
    14041 ggcttcgcga gcgtgacctg cacctgcggg ttcgacgggg cctcccgtcg caaggaggtg
    14101 cgggccaaga tcgaggccct caccgggggc tcctcggagc tgaagaggaa cctcttccgg
    14161 tcgatgtcca acgtgaacgc cgagtacctg gcctaggagt ttgtcgggct tgcccatata
    14221 aataatagaa aaatcgcatc tgtgcgattt ttctaccttg caaactccaa aggagccgga
    14281 taatcgggat tttagcgctt atcggcgcta ataatccaca agtccgccag gctcctagcc
    14341 cggcccggcc ggcgcgaagt acgtgaaggt ttttccgccg gggactgtat cggcccgggc
    14401 ggcccgggat agtatccctc catgctcgac ctagccttca gcgtcctgct ggccgccctg
    14461 cccgccgcga tcctcctggc ctggttccgc cgccaggacc gggcccgccc cgagcccgtg
    14521 ggcctcctgg gccgcagcgt cctctacggc ttcctggcca ccgtgccggc cgccgccctg
    14581 gagctcctcc tcctgcgctt cggcccttcc gggccccggc tcctggccgc ggccttcgac
    14641 gccttcgtgg tggccggcct ggtggaggag ggggtcaagt actttttcgt gcggcgctac
    14701 ctcctcgggc gccccgagtt cgacgagcgg atggacggga tcctctacgc ggcctgcgtg
    14761 tccctgggct tcgccttcgc ggaaaacatc ctgtacggct acgcggaccg gacggtcctg
    14821 ttcttccggt ccttcaccgc cgtgcccatg cacgccgccg tctccgggat cctgggctat
    14881 tggatcggcc gggccaagat cgagggaggc cgcgcggcgg cggagagcgc ctcgtccgac
    14941 cgtgcccccc gggccctccg ggggctatgg gaggcggcct tcctgcacgg ggcctacgac
    15001 tttttcctgt tcacccgatc ggcctgggcc ctgatggcgg ttcccgtcct gctggcggcg
    15061 atcctccgcc tgctcttcct ggtcgcggac gcgaagcgac gggatcggaa ggggcttgaa
    15121 gaggagccaa agttatatta aactaacatt atagaatcga accggttatt cccgggaggg
    15181 aagccggaaa ggatacatca tcatggaagg aaatattgga ttcgccctgg gcctgacggt
    15241 gttcgcgggg ctttcgaccg gagtgggaag cctgatcggc ttcgtttcca aggagctgaa
    15301 gccgcggttc ctgtccgcgg ccctgggctt ttccgcgggg gtgatgatct atgtctccct
    15361 ggtggagatc ttcgtgaagg cccgggattc cctggccatc gggatgggag cccggctggg
    15421 atacttcgtc accgtcgcgt cgttcttcgc gggaatgctc ctgatcgccg tcatcgacaa
    15481 gctcctgccg tcctacgaaa acccccacga gatgaacacc gcggccaagc tggaagggaa
    15541 gagcgagcgg gagaaggcca agctgatgcg gatgggggtc ttttccgccc tggccatcgc
    15601 catccacaac ttccccgagg ggctggccac cttcatgggg gccctcgcga accccaccct
    15661 gggggtgagc atcgcggtgg ccatcgccat ccacaacatc ccggaaggac tggccgtgtc
    15721 ggcaccgatc tacttcgcga cgaaaagcaa gcgcaaagcc ttcctcctgt ccttcctgtc
    15781 cggcttcgcg gagccgatcg gggccctcct ggggtacttc gttctccggg cggtcttcaa
    15841 cgaatccatg ttcgggatgg tgttcggggc ggtggcgggg atcatggtct acatctccct
    15901 ggacgaactc ctgcccacgg cggaggagta cggggagcac cacgtggcga tcacgggggt
    15961 gatcctgggg atggtcgtga tggccgggag cctcctactg ttcagctgag aggcgcaggg
    16021 ccacgtcctc ccattccagg tttaaccgtt ccgcctcggc gtccagggcc gcggcggctt
    16081 cctggaggcg cttcatccgg gccccgtcgg agtagttttc cggcttgccc atctccgcct
    16141 ccgcggccgc cttctgggcc cccagggtcg cgagccgggc caggatctcc tcctcccgct
    16201 tctggagcct gcggagggcg gccttgcggg ccttctcctc ctcccaggac tgggcggtcc
    16261 cggagggtcg cggggagccc gtcccggcgg gctgtccggc ccccggggcg gccttcccgg
    16321 gggagacggc cgggccggac cgtaccgggg ccgccttgcc ggcaccggcc gcggcttccg
    16381 cgtccagggc ggcgcgcttt tcccggtagt agtcgtatcc cccggggtag aaccggggca
    16441 cggatccgca ctccagctcc aggacccggt cggccagctc ctccaggaag aagcggtcgt
    16501 gggacacgaa gaggaccgtg ccctcgaagg ccttcagggc cgacaagagg acgtccttgg
    16561 agtccaggtc cagatggttc gtgggctcgt ccatgaccag gaggttggcg gggtggagga
    16621 gcatcttgag caaggccaga cgggagcgct cgcccccgga caggacacgg acgctcttgt
    16681 agatgtcgtc cccccggaag aggaaggccc ccaggaggcc ccggatcgag gggatcaggt
    16741 ccgtggggca gaccgagaga gcctcctcct ccaccgtccg ggtgccgtcc agggattccg
    16801 ccacgtcctg ggcgaagtag gccgtggtca ctccgcttcc cagccgcagg gtcccccgga
    16861 agtcacgatc gaccccggcg acgaggcgca tcagggtgga cttgcccgcc ccgttgcggc
    16921 ccacgaaggc caccttccac ccggaatcca gggtcaggga gaggtcccgg accacggact
    16981 tgtccccgta ggccttggac aggccctcga cggtcagggc gatgcgcccg cacttgggcg
    17041 ccggcgggaa ccggaaatgg atgcgcttca tgccctcggg gatctcgata ggcacgatcc
    17101 gctccagctg cttgacccgg ctctgcacca gggtcgcctt ggaggcctgg tagcggaagc
    17161 gccggatgaa atcctccagt ttggcgatct cctcctgctg cttctcccag tccttgagca
    17221 gggtctcgag ctcctgggcc cgggtccgtt cgtaggcgga gtaggtcccc tcgtagcggg
    17281 tcaggcggcc gttccagagc tcgtagacct gggtgaccgc ggagtccaag aaggagcggt
    17341 cgtgggacac caccacgaag cccccgggaa agccgcgcag gaaatcctcc agccagtccc
    17401 gggcctccag gtccaggtag ttcgtgggct cgtccaggag caggatatcg ggattctcca
    17461 ggagcacctt ggccagggcc acgcgcatct gccagccccc ggagaactcc tccacggggc
    17521 ggtccggatc gccggccttg aagcccaggc cctggagcac ctcccggatc cggtccttcc
    17581 gccgccagta gcccgagcct tcgaccgcct cgtggaggcg gtggtgctcc tccaccaggg
    17641 ccagggtccg cgggtcgtcg gccacggacc cttccaggcg gcggccgatc tcctcctgct
    17701 cccggaccag ggcctccacg gactggtagg cccgctcggc ctcctcgtag accgtgcagc
    17761 cccggtagac caccccggtc tggggcaggt aggagatccg ggcccccttc tggatggccc
    17821 ggtccccgcc gtcgggggcg atgagccccg cggcgatctt gagcagggtg gtctttccgg
    17881 ctccgttcgg ccccgccagg gcggccttgg ttcccggcgc caggaagagg ctcgcgttct
    17941 tcaggatgtc ccggtgcccg aaggccaggg agacgtcggt gaactgtacg aacgccatgt
    18001 ctagttggcg gacctccgga accggatgct cagggggggc tcggacaggg tgttcaccac
    18061 ggaccactcc ttgtcgatgg gacgcacgag ctggaggtcc agcccgccgg gggtggctcg
    18121 gtacacgaaa tccgcccggg gcctcgcgcc accgtcgaag aaaagggaca ggcctccgtc
    18181 ccattccccc gccaggaggg ggcccgggaa gagccccagg acgaccgcgc cggcctcgcc
    18241 ggaatattcc ggtatgccct cggggatcag cttgtcgtag gccgcccagg tgaaacggcc
    18301 ggatcgggag atcgtcaggg cgccccactc gtcggagacg aagcgctccc cctcggcgat
    18361 catgcgggcc agggcgttgg cccttcggag ctcctcccgg cgcacgatct cccggatgtc
    18421 ctggtcgaag accgcgaagg ggtgggttcc cgccccgtag tcggtctcga tcgtcacctc
    18481 cagggattcc gggtccggga agcggagggt cagggggagt ccctcgaacc ggaaggaccc
    18541 gtccgcctgg ggaaggatgt tcgtgtagtt gaagaaccgg gacgccccgg ggtactcgat
    18601 ccggatctgc cgccgcacag cgtccgtgaa gatcccgaac tgcgggccga ggcgctccag
    18661 gtccacccgg ccctgttcga tcatggtctt gaaatacgcg gggcgccagg tccgggagaa
    18721 gaggaggtcc aggtccgcgg ccggatccgg cccggatacc gccgcgcccg cggcgggacc
    18781 ctcggattcg tcgtagatcc tgagctggtt cgagaagacg tagccccggg tcccgtcctc
    18841 ccccatggcc aggtaccagt ccccgggcag gggttttccg cccgtggtga cctcctcgcc
    18901 ctcggtcttg gccaggagtt tgatggactg gtcccggcgc atccggtaca cctggttggc
    18961 caggtttctg ggctcgtccc ggagtacgag gccgtcccgg agggccacgg cgtagaggcg
    19021 ggcgctgggt tcgaattccc gggcccgggc ctcggcttcc cgtttccgcc ggaagagttc
    19081 gatcttccag acgggcacct cggccttctt ccgggtggcg gggttttcca cgacgtaggt
    19141 atggttgatg ttcgacttga tgaagaccgg caccaccgtg cctgcgggta cccgttcggg
    19201 ggagggggcc tcgagccccg ttccggtgtc ctgcccgtcg ggggtcgctt cctcctcggg
    19261 tacgggcgcc gcctccgtcc cctcgaagta ccagagcacg accccccagc ccagcctgcg
    19321 ggaacacccc gtcagggaga gggccaggag caaggctccg gcgaggagga ctaagctgcg
    19381 gcgggcggga ctcatggggg ccagtctaca acgcgtccgg ggaattcatc aatacgaacc
    19441 gcgaaggctt gcgcggacct ctccggggcg gacatacttc aaggcatgga ggacgccatg
    19501 gcagccgaaa ccggtttcgt cgcgggcatc gacctggggg ccaccaagat cctggcggcg
    19561 gtcttcgacc gggggatgaa ggtccgggcc caggccaagg tcaagacgcc cgggagcggc
    19621 tcggcggacc aggtcctggc ggcaatcctg tccgccctgg acatcgccct ggccgagctc
    19681 gacctgaaac ggagcgacct ggagggcctg ggggtggcgg tcccctcccc ggtgaaccgc
    19741 aggaagggca tcgtcctttc caccccgaac atggggatga aggactttcc cctccgggac
    19801 cgcctggccg ggctctcggg tctgccggtg gccctggaga acgacgtcca ggccgggacc
    19861 ctgggggagc tccggggggg agccctccgg gggaagaagt acgcggccgc cttcttcgtg
    19921 ggcaccggga tcgggggcgg cctcgtcctg gacggcaggc tgtaccgggg ctccacgggc
    19981 tccgcggggg agctggggca catgatcctc caggacggcg gggccctgtg cggctgcggc
    20041 cgccggggct gccttgaggc cctggcctcc cggacggcca tggcccggga cgccgtggca
    20101 gcagcggcct ccggcaaggc cccgatcctg ctggaccacg cgggcacgga cttccgcaag
    20161 taccggagct ccgccttcgc ccggagcgtg gagtccgggg agaaggcgat cgagcgaatc
    20221 gtggagaggt ccgcgtactg gctgggggtc ggggcggcca acgtcaccgc ggtgctcaac
    20281 cccgaggcgg tgctcctggg gggcgggatg gtggcccgct tcccggatct gtacaaggat
    20341 atagcattca ggtccctgaa ggaccacctg atgccggccc tggggggcac ggtggagctc
    20401 ttgatcacca agctggggga cctcgcggtg cctctgggcg cggcctgggc cagccgcgat
    20461 ctcgcggccg gccgcgaatc cgcggccgcc cgggaggtca cgggcgcccg ggaggacatg
    20521 gacggacagg aggcggcgga atgagcgggc agaaggccct ggcggtggtg gaggtggggt
    20581 ccacggggat acggctgatc gtggccgccg tggaagcctc cggggaatac cgggtcctgg
    20641 accacgcggg caagccggtc cagctgggcc gggacgtatt cgtctcgggc tccgtcagcc
    20701 gggcttccat gctggaggtc atcgccgtgc tccggggctt ccgggagctc ctgcggacct
    20761 acgggatcga gcccaaggac gcccgggtga tcgccaccag cgccctgcgg gaggcggaca
    20821 accgggacac cttcacggac cggatagccc tgcagacggg gttctcggtg gacatcgtgg
    20881 aggacatcga ggagaaccac ctgatgtacc tggcggtcca gaaggccctg ggggaggagt
    20941 tccgctccct ggcccggggg aacgccctga tcatcgaggt ggggggcggg tccacggagc
    21001 tcatgctcct gcgccggggg cgaatgacgg ccgcccactc cctgcggatc ggcaccatcc
    21061 gggtggacga gcaggtccgg gccgccatgg ggtctccggc ctacctggag cgctacctca
    21121 cggacaacgt ccgcaccacc tgcgacaacc tcgactcgga gatgcccctg gccacggtcc
    21181 ggaatttcgt ggttatcggc agcgacgccc gcttcgcggc ccgggccgcg ggtacccggt
    21241 ccgaaagcgg ggactactac gtcgtgcccc gggaatcctt cgaccgcttc gtcggggaga
    21301 tccaggagct ccgggtcgag gagatcgcgg ccaagtaccg ggtggcgcac ggcgaggcgg
    21361 agggctattc cgtgggtctc ctgatcacca agctcttcct ggagcggacg gccgcggagg
    21421 agctgatcgt ccccttcgtg tccatccggg acggatacct gatgcggatc acccagggcc
    21481 cctcggcctc cctggagaac gagctgcacc ggcaggtggt ggcctccacc ctggccctgg
    21541 gccgccgcta ccacttcgac gaggagcacg cccagcacgt ctcggagctg gccctggagc
    21601 tcttcgaccg cctggcggac gagcacggcc tggggaagcg ggagcgcctg ctcctggaga
    21661 tcgcgggcct cctgcacgac gtcggcacct tcatccgcac cagcggccac cacaagcact
    21721 cggagtacat catcgccaac agcgagatct tcgggctcca gcgggacgac ctgaccatca
    21781 tcgccaactg cgcccggtac caccggaagg ccagcccctc ctcggcccac cccagcttca
    21841 tgagcctgtc ccgggaggac cggatcctgg tcctcaagct ctccgccctc ctgcgggtgg
    21901 cggacgccct ggaccgggga catacccagc gggtccggat cgcggacacg gagcgcaagg
    21961 aggaccacct ggtcatccgg gtcgaggacg ggggggagct caccctggag cggttgtccc
    22021 tggaggaaaa aggggatatg ttcgaggacg tgttcggcat caaggcggtt ctggcatgat
    22081 tcgtgaccgg ttcttcaatc gcgagctttc ctggctggag ttcaacgacc gggtcctgga
    22141 ggaggggctg gatccctcca atccccccct ggagcggctc aagttccttt ccatcgtgtc
    22201 ctccaacatg gacgagttct tcatggtccg cgtggcgggc atcaaggccc agatccggga
    22261 ccgggacgag acccgggacg cggcgggcct ctcgccggag gaacagctgg ctgcgatatc
    22321 cgcccgggtc cgggagatcc agacccggca gtaccgctgc ctgaccgagg atatcttccc
    22381 cgccctggcg gccgaggggt tgtgcgtcgt gcgcccctcg gactggacgg gctcggagct
    22441 ccggtggctg gaggcctggt tcgcccgcaa catcctgccg gtcctgactc ccctgcgcat
    22501 ggacgacggg gagggcgtgc cctccacggg caacctccgc atgcacgcgg ccttcctgct
    22561 ggaacccccg gaggttcccg acggggcgga tccggccgga ggggctccgg agggggccgg
    22621 cggaccgggc ggtgtctccg gcctcatggg ggaggccccc gacgggaggc cccggctggt
    22681 catggtcacg gtgccgccca acctggaccg tttcgtccgc atcccctcgg agtccgggcg
    22741 cacccacctt gccctcctgg acgaggtgat cctgaccctg gggcacgccc tgttcccggg
    22801 ctaccgggtc ctggagcgga acctcttcaa ggtggcccgg gacgcggcca tcggcgtgga
    22861 cgagggccgg gatgacgact tcgtggcggc catggaggag gtcctggcca accgccagaa
    22921 ttcctggccc gtgcggatga ccctggggct ggagagcccg gccctccggg aacggctccg
    22981 ggaggtcctc gggctctcgg aagaggacgt gtacgaactg gccggaccga tcgacctccg
    23041 gtccttcatg gagctggcca ccatgaaggg cttcgaccgc ctgcgctacc cggcccgcaa
    23101 acccgtggag ggctacgagt tctccgagga caactccatc ttcgaccaga tccgcaaggg
    23161 ggatctcctt ctgcatgtgc cctacgagtc cttcgccccg gtggaggagt tcgtgcgcgc
    23221 cgccgcggac gaccccaacg tcctggcggt caagatgacc ctgtaccgga cttccgggga
    23281 ttcccccctg gtccggtccc tgacccgggc cgccggcaag gggaagcagg tcacggtgat
    23341 ggtggaactc aaggcgcgct tcgacgaggc caagaatatc gagtgggcct ccagcctgga
    23401 gcaggccggg gcggtggtgg tctacggagt ggcccgcctc aaggtccacg ccaaggcctg
    23461 cctggtggtc cgccgggagg aggacggctc catcctccgg tacgtccacc tgtccacggg
    23521 caactacaac gacaagaccg cccggctcta ctcggacctc tccttcttta ccatcaacga
    23581 gaccctctgc cgggaggcgg ggatcttctt caacatgctc accgggctgt ccgcggtgga
    23641 ggagctccgg accctggcca tggccccctt cagcctcaag cggcggatca tcgccatgat
    23701 cgaccgggag gcggactgga gttccccgga gaccccgggc ctgatcatgg ccaagatgaa
    23761 ctccctctgc gatcccgacg tggtcgccgc cctctaccgg gcctcccgca agggggttcg
    23821 gatcctgctc aacgtccggg ggatctgcat cctggtcccc ggggtcgagg ggctctcgga
    23881 gaccgtcacg gtggtatccg tggtgggccg gttcctggag cactcccgga tcttctactt
    23941 ccggaacggg ggggccgagg agctctacct ggcctccgcg gactggatgc cccgcaacct
    24001 ggaacgccgg gtggagctca tgttcccggt gcccgatccc gctgcccggg accgggtgct
    24061 ttccatcctc caggcctatt tccgggatac cgtgaaggcc cggatcctcc ttccctccgg
    24121 ggcgtgggag cgcaggctcc ccgagcccgg ccgggagccc ttctccgccc aggactggtt
    24181 ctgggagtcc gagcggaacc ggttccggga ggagtccgag gccgaggaga gggtcttgac
    24241 cgtgcgcagg cgagccccgt gaacgatccc ggccggcccg cccgcggccc gggccgggga
    24301 ccgcggggag gatttcggaa tcgaggggcc cgggacgcgg cggtggtccg tatcgtgggc
    24361 ttgccgaacg cgggaaagag cgccctgtac aacgccctga ccggtgccta cagcgtcgtg
    24421 gccaattacc cgaacaccac cgtggatccc gagcggacga tccggaggga ggggggcgtt
    24481 ccggtccgct acgtggacga gccgggggtg gacgggttgg ccccggagtc ggaggacgcg
    24541 cgcctgctcc tggaccggct gctgtcggag aggacggacc tcctgctctt cgcggccgac
    24601 gcccggcgcc tggaatccgg cctggccctg ctctgggagt tcctgttcct ggggatcccg
    24661 acggtcctgg tcctcaccgg tatcgacgag gcccccgtct acggcgtgga ggtggagtat
    24721 gcggccctct cccgcctgct gggcgtcccg gtcgcggcgg tcagctcgga ggagggccgc
    24781 gggatccccg aactcaggtc ccgggtgcgc acgctcctct ccccggccgg cgcggcggga
    24841 acgccccgga ccggcgggga agcggcgtcc gtcgcaccct ccccgcctcc cgagcccccc
    24901 ctgcctccgg ggatccggga cgcccgggag acggtgcgtg cggccctggc tcccggggtc
    24961 cggagccccg gcgccgcggc cctcttcctt ctccgggcag acccggacct ggcccgggcc
    25021 taccccgggg ccggccggac cgcggctgtc cggaccgccc tcctgggcct gcccctggag
    25081 cgcctcctgt cccggggccg gacggactgg gcccgccgga tcgtgaggtc ctgcgcgagg
    25141 atccgggggc gccggcccgg gagcgccgcg gaccggctgg ccgccctggc gcggcatccc
    25201 ctctggggct tgcccgtgct gggcgccctc ctgctgtcgg cctatttcct ggtgatgtac
    25261 gcctccgtgg acctggccgg gtggatggac tcgaccctgg ccgtcccggc cgcggcctgg
    25321 gtggcggatc ggatagcctc ccccgggatc cgggacttcc tggtcgggga cttcgggctt
    25381 ctgaccctgg gggtgttcaa cgccctgtgc acggtcctgc ccatcctggc ggtgttctac
    25441 ctcttcttcg gaatcatcga ggactccggg tacctgccca acttgatcgt cctgtcccgg
    25501 accgccctgg gccggatcgg cctttccgga cgctccgtcc tgccggtgat cctgggcttc
    25561 gggtgcaaga ccatggccac cctctcggcc cgctccatcc cggaccggaa ggaacggacc
    25621 atcgcggtct tcctcatcgc cttcggcatc ccctgctccg cccagctggg cctgaacatg
    25681 gcaatcctgg cccgggcggg gacgggagcc ttcctgatcg ccctgggctt cctgagcgcc
    25741 gcggagctgg gggcgggggc ggtcctgaac cgcctgcttc ccggcgagtc ccgggggggc
    25801 ttcgtcctgg aacttccccc cttccgggtt ccggatcccg gcgccctgct tcgcaagacc
    25861 gggtggcggt tggcccgctt cgtccgggag gcccttcccg tgttcgccgc cgccgccgtc
    25921 gccctgtacc tggcggacgg atcggggatc ctcggggcct tccgggactt cctggcgccc
    25981 ctggtctccg gctggctcgg cctaccgcct tccatggcgg acgccctgat cctgtccatg
    26041 gcccgccacg aggcggcggc ggggctgatc ctgggcatgg cggagcgggg cctcctggac
    26101 cggacggcca tggtcgtttc cgtgacggtc acgaccctgt tcgttccctg cgcggccaac
    26161 atcgtggcca tcgtgaagga actggggaag cgccggggcg cggcggtagt cctggcggcc
    26221 aacgccctgg ccatcaccct ggggggtgcc ttgaacggcg tccttcgctt tctgggcttc
    26281 tgagccggag ggatcatgac ggacacgagg aacgaggacg agcacctgga gcggatctgg
    26341 cgcatggagg aggagggagg cgggagcctg accctgctcc ggacccacat ggggaaggac
    26401 tacgatcccc tcgatatcga gtccctttcg cggcagggct gcctggaggt gcggggggac
    26461 gaggtcgcct tcacggaccc gggccgggag cgggcccgcc gcctgatccg ggcccaccgc
    26521 ctggcggagc tcctggtgcg ggacatcctg ggggtcccgg atttcgaggc cggggcctgt
    26581 gagctggagc acctggcgga caccaaggtg gtggacggga tctgcaccct cctgggccac
    26641 ccggcctgct gtccccacgg cctgcccatc ccccggggcg actgttgccg cgccggggcc
    26701 cgggaggtga cctcggtggt ggtgcccctg acggagctgg agccgggcaa ggtcgggcgg
    26761 gtggtcttcc tggaagcctc ggacgaacgg gagatccgcc tcctgggtac ctcccagatc
    26821 cggccgggga cggagctacg ggtgcgccag cgcaggccct gctacgtggt ggacacgggc
    26881 accgccgcgg tggccctgga cgaagcggtg gccgccaccg tgaaggtcct ccccctgggc
    26941 tgattcccga gcccgggaga cccgggtccg gagcgccggc ggcgcctctt ccccatccgg
    27001 gattcgtccg atactatacg taacgcggcg gccgtgcgcc ttcgccggtc cggacccggg
    27061 ctcgcccgcc gcgccggatc tcaacctcaa ttccatcggg agcacctacc gcatgcgcct
    27121 gtccgccccc cgtcccgccc tcgtcctcgc cctctccctc ctccttgccg ctgtcctgcc
    27181 cgcccagacc ctggcgggct tcgacacggg gctctatcgg atcgagcggg acgggtcctc
    27241 ccgacccctc tggctgggcg gggaggtcaa gaagatcctg gccaccgagg ccggctggta
    27301 tttcctgagc tcccgggggg tggtccacag ccgcgacctt tcggccttcg aggagcggaa
    27361 caagggcctg ccggtcaaga cgatcaagcg ctacgaccag ggggtcaaga gcttccagta
    27421 cgagatccag gatttgaagg acctggaggc cgacccctac gacccccgga ccctggtgac
    27481 ctgcaccaag gacgccgtgt tcctgacccg ggacgggggt cttacatgga ccgccctgcc
    27541 cgcggctccc tcgtggtcca cgggcctcaa ggccgcggcg gtcgtttccg ggacggagaa
    27601 cctgattttc gcctcccacc ccatccgggg cgtcttcgtc cgtcccctgg gaaatcccgg
    27661 ggcggcctgg acggaggcgg ggggcctgga cagggccgcc ggctccacca gcccggacga
    27721 ggtttccgac atcgtcgcgg ccaaggggcc cgagggggcc cggatctggg cggccaactc
    27781 cttccttccc cgcctgtacc gctgggattc cgcgcggcgc tccttcgcgg tggtccacgc
    27841 ggacgaccgg gacttcgccg ccttcgagag cctccaggtc cgggaggacg gggtcctcta
    27901 cgtgacggac ggggagatct accgctacga tccccgctcg ggccggcacg aggccgagcc
    27961 cggagccctc cgcgcggtcc tggcggcctc ccgggcccag ccgggccagc tggaatcctt
    28021 ccacggcgtc gactccacgg gcaagcccct ccagctttcc gagctctggc tggccgcctt
    28081 caaggaccgc aagccccacc ggccggccgc ggagggccgc cacggcctgt acctccagac
    28141 gggattcatg gtgaacccgg aaacccgggc caagtacctc ggggtcctgg gccggcacaa
    28201 cctggactcc gtggtcgtgg acctcaagga cgacctgggc cgcctgcgct tcgagccccg
    28261 caccgaactg gtccggcgcc tgggacgctc cgtgaacccc ctggacgtgg aggccttcac
    28321 ggcggagatg aaggcccggg gggtgtacct gatcgcccgg atcgtggtct tcaaggacaa
    28381 ccacatgtac aaggccgcgg ggggaaagta cgcggtctgg gactccgcgg agaaggcccc
    28441 ttggcgcggc tacttcacgg aaaaggtcga ggctcccgca cccgccgctc cggtaccctc
    28501 aacgcccccg gccctcctgg ccccttcccg gcccgcgccg ggggcctccg tcgcgcccgc
    28561 cgcccccccg gctcccgctc cgacctacca gcggaagtac atggaagagt attgggtgga
    28621 cccctatagc gaggagatct gggagtacaa cgtggccatc gcccgggaga tcatcgcccg
    28681 gggcttcgac gaggtgcagt tcgactacat ccgcttcccc acggacgggg agaacctcgg
    28741 gaacgcctcg taccgctgga gggaccccgg catggacatg gagagcgccc tgatgtcctt
    28801 cctgggctac gcccggcgga acatcgaggc gcccatcagc atcgacatct acggggccaa
    28861 cggctggttc cggtccgggg tgcggaccgg ccaggacgtg gagctcctgg cccggtacgt
    28921 ggacgtgatc tgccccatgt tctatccctc ccatttcgag caggacttcc tggcccagcc
    28981 cccggaggag ctccgtccct accggatcta ctacctgggg accctccgca acaccttcat
    29041 cgcccgaaag cgggtggtcg tgcgccccta cgtccaggcc ttcttcctga acgtccgcta
    29101 tgaccggaag tggtacaacc ccgactacgt ccgccggcag gtggaggggg tgcgggactc
    29161 ggcgaacctg gggctcacct tctggaacaa tatcggccgc tacgacgaca tccccgtcct
    29221 ggatgtccgg gggaaccgga ggctggcggc gggaccggag gccctccggc ccaagggcgt
    29281 ccagctggac tagtggggag ggggcggcct gccgcatgcg tcggccgtcg agcttgacgg
    29341 gcgcggcggc tggaagtagc ttgtaggtac cgtgaaaccc tcccagccct tccgagtccg
    29401 attcctggcc ttcgcggccg cggccctcgc cgccttgctc ctcttccgcc tgatgcacgc
    29461 cccggggacc ctggggctcc tggtctggaa tccgagctac cgctccttcg cctacgggat
    29521 cctggccctg ggggcggtgc ccttcctcct ggcgatcgtg tacgaggtcc tccgggggat
    29581 cccctccttc cgccggcgcc gggccgcggg cctggtctcc atgggggttt ccgcggtctc
    29641 cggggcggcc gccctggcgg cgatcctgtt tctcgcgatc gccccgaacc gggtcccgga
    29701 tcccctcccg acgccgagga tcctggatcc ctcccggggc atcgtcgtcc ggccctccgg
    29761 ggacgggggg ccctacctcc gcctggcctt ctcctcggat ccccactggg gccgggaaca
    29821 gagcgacgag gccgcccgaa cgcggatcct gacaagcgtg gacgccgggg actacgacgc
    29881 cttcttcctc ctcggggaca tctccgagca gggctttccg gagtcgggct tccgggaggc
    29941 ggccctggac ctggaaacgt acctgacccg ggtcccggtc cgtccgatga tgggcaacca
    30001 cgacgccctg gtcaacgccg cgggccggtg ggcccggtat ttcttcccct cgggccattc
    30061 ctcggattcg ggttcccccc tttactaccg gatcgacgcg ggccgggccc atatcctggt
    30121 cctcaacctc ctctgggggg aggaggactt cgaccacgcc cagcgtgcct ggctgacgga
    30181 acagctggag tccatccccc ggcaggacgc ggtggtcgtc ctgtcccact gctatttcta
    30241 cgcctccggg tacgacgacc ccgagtccgg aagcccctgg tacgaccatc ccgggaccat
    30301 ccgggaggtc gtacccctgc tggagacgta cggggtggac ctggcggtct ccgggcacaa
    30361 ccattaccag gagctcctgg agcacaacgg cgttacctac gccgtgatcg gggccatggg
    30421 cggggtgccg gatccggccc cgacctaccg gtcccccgcc tcgagatgga tccgggtctc
    30481 caccttcgga ttcctggagg cccggttcta tccggaccgc atcgagctgg ccttccggga
    30541 cgcggacggg ggcgagctgc accgcgcggt ggtttccgcg gagtaggggc gatgggatgg
    30601 aaagcgtcgc ggacccggcc ggctccggca gcgcccggtt ccaggaagcc tccttcacgg
    30661 ccctggaccg ctaccggggc aacccggccg cccccctgcg gttcctcctc ttccgggggg
    30721 gacttccgga cctggcccgc cgcggccagg agctggcctt tcccgggctt ccctacgcgg
    30781 acgcggtctc cgcgaacgcg gtctccggga taccgcggcc cggggaatcc ggtcccgttg
    30841 acggcgcctt gccggatccc gtctccgtgc cctgccgcta cgtctgcgcg gaggcggacg
    30901 aggaccccga gccccacgcc tacggccttt tggacctgag ctacgacccg gagacccggc
    30961 ggtaccggca tccgcgacag cgtttcccgg acccccggga gcccgtgatc tacccctgcg
    31021 acgcccccga cgacctcacc ctcttcgaga ctgccgtcct cctggcccgg tatccctacc
    31081 gcctgccgga ggggggcttt gtcgtggatg tcccgaggaa cctctccccg atgttccagc
    31141 gggacctcct gaccctgatc ctcaccggcc gggatcccgg gtccgccctg gaatggctgg
    31201 gccggaccgg gttcctggat gcctactggc ccgagcttgc ggccctgcgg ggggtgagcc
    31261 attccaagga gtaccatccc gaaggggacg tctgggccca caccctggag accttccggt
    31321 atcgcaagct ctcggagttc cggctttccc tgggcctgct cctgcacgac acgggcaagc
    31381 ccgaatcccg gtccgcggag ggccgccgct tcgaccagca cagcgagatc ggggcccgcc
    31441 tggcccgccg cttcctggag cgcctgggat tcgcccggga cctctcggag gatgtggagt
    31501 tcctggtccg caggcacatg atgcccgcgg ccctcccgcg cctgcccctg aaccgggtgc
    31561 aggaagacct ggagcacccc ctcttcccca tcctcctgga gctgtaccgc tgcgacgagc
    31621 tgtccacctt ccggggaccc gacgggtact accaggcctg cgcggcctat cgccaatacc
    31681 tgcggaacgt gcgcaacccg taccggaacc cggacggccg gaaggtccag aggatgttcc
    31741 ttgaatagga ggtcccgtga aggaagccta tcgctgcgtg tacatggagg acgccctggt
    31801 cgtgaagagc ctcctggact ccgcgggcat cccctctcgc atcctgagcg gggacatgct
    31861 ggacgtgaat cccttctttt ccgccaccgt ccagggtgtc cggatcctgg tctccgacga
    31921 ggactgggag gacgcccggg cggtggccgc ggacttcaag tcccgtcggg aggactgagc
    31981 ttgtccggac tcaccgcgga gttcacggag ggaagaatcg aaccggagcg ccagatatcc
    32041 ttttctagaa attgagccga tttcaaaagt cggtcacttt tgaaatcggc tttgcgattg
    32101 ctcgcggttg cgttgcaacc gctgcgcaaa tcgcgatttt caaggtagct ttttcaaaac
    32161 tctctgagtt ttgaaaaagc ctctagaaat tgaatatagc ccgacgattc tccgcgccga
    32221 tccttcgtag ttcctccatc aagggccttc cgggatccct cttccgggac agctctaggg
    32281 agaactggtt catggaaaag tgactgaaca ccaggacctc ctcccggacg gccgccgggg
    32341 cttcccggta gagtcgggag tactcccgga accaggaccc gaagatcccc ttgggcttga
    32401 gctcggaacg gccggagatc cagaccggga cgcccagggc ctcgtgaagg gcccgggcga
    32461 agagggccat ccacgcctgg ctctccgcca gcagggcctc cacggccccg cttgcgtcca
    32521 gattcctgag aagggccagc tccgcggtct tgggggtgtg gacgggggtc ttgttgacga
    32581 tgaggacatc ccgccggaaa tccgtatcca gctccctgcg gaagaagccc tccgccagct
    32641 ttccggactg gcctaccagg tatcggagat tcgcggattt ttgctcctgc ttgcccgggt
    32701 tgtccgcgat caggatccac cggggacggg actccgggcc gatgtcgtcg agggcccggt
    32761 tgtacacgac gggggtttcc acgacgtagt cgtcgtagcc ccgggcgatg cggagcctct
    32821 cctggatatc ccggagctcc ggcacggcgg cggtccacag ccggaccttt tcccggaagg
    32881 cgtccctcag ggctgtgaat ttctcgtacc ggcgtcgatc catgccgggc attgtgacgg
    32941 acccggcccg aagccgcaag ggcggccggc aatccgagct catgtggata ttgcggcccg
    33001 ccgagcatat actgaagcca tgaaatccac acgccgcctt atgatcgccc tggtcctgct
    33061 cctgggcgcg cacgccgcct tcgggcaggg acggttccgc aacatcctct cgctcaaatt
    33121 cgagtacttc accgaccagc gcttcggatt ggagtgggag gaagtcttcc tggcccccct
    33181 gcccaagggg ttctacctgg tgtccgcggt ctccggcacg aacacgagct tttccgacct
    33241 caccggggga cgcctggggc tggccttcga tctgcccggt tccttctacg gagagttctc
    33301 ctacgagttc gaatacgact ggcgggtggc ccagccgaaa cacacccccc tggtctcctg
    33361 cacctacgag tcgggacccg cgatggcgag cctttcgttg tccggtgagt tcaccccgga
    33421 cagcgcaggc ggggtcctct cgccctccct gaagtatacc ctgaatcccc ggtttttcct
    33481 gtcctcctcc ctgttcctcg ccttccatca ctactccacc tcggaaagct tcttcaactt
    33541 cgcggccctg gccacggggg agtacgggct gggaccggag atattcctgt ccctcggcgg
    33601 taccttcggt acggtgtacg agcccgacgc gcgctacgag aaatggtccg tcctcggagg
    33661 agtcaaggtc gtgccctcga attcggtcgc ggtgaagtgt ctcgtcgagt tcgagaacgc
    33721 cgacgccgcg ccggtgaacc cccacaacat catctccgcc cagatcgtgg tggacgtcaa
    33781 gttccgcgca aaagagtgag gaaggcccgc ccgggccgga gatgtccgcg aagccccggg
    33841 ggattcgttc gggaggcagc atggggatcg agacgatcgg cgcggcgctc gtattgacgc
    33901 ccgagatcaa gcacctggat atcctgagct ccaaggagtt cgcggaacgc ttcgctccgg
    33961 cggcgaagga cgcgcggacg gtcatcctgg acctgtcgca ggtcgagttc gtggacagtt
    34021 cggggctcgg cgccctgctc aacctcgtcc gggacaccct cggaaggggc ggacggatct
    34081 cgatctgccg tccccagcct tccgtcaagg tcctgttccg catggtccag ttatcgaagc
    34141 tcgtctccat cttcgacggg aaggacgcgg ccctcgcctg ggcggagaca tgagcggagt
    34201 ccctcccgac ctgataccct gggttccggc cgccgcggta tccgtgtcgc tctgcgcact
    34261 tgccgcggcc gcggtacttg cggcccggcg aggtcccctc ctgcccctcg atcggccgga
    34321 gtaccgcgag ttcgccgcct ccctggacgg agccgtcgcc ctgatctccg gggacctgga
    34381 gaaggtcctg tacgcgagcc ccggtttcga gcccctcttc gggtcgactc cggatcgcgt
    34441 ctccttccgg cggctggagg gcgtcgtgca cgccgaggat ctggaggccc tggagtcggc
    34501 cctctccgca ggccgcatgc ggcccttcga ggtggaattc cggatccggt cggagggaga
    34561 gccgcgctgg ctgcggatgc ggggcttccc ggtggcggga acggggagga aggccgcccg
    34621 caggatcgcc gtgtgggtgg aggaccggtc cctgcgcaag accgacgagc tcaggctggt
    34681 ggaggccctc gactacgagg tggccgtggg ggcccgcatc cagcaggccc tgctcctggg
    34741 gagtccggag cgggcgtacc cgggcttcga tctggcctcc atgaccctgc cttcccagaa
    34801 gatcgacggg gatttcatgg atttcttcga cggcggaccc gaggtgctgg atctcatgct
    34861 cggggacgtg atgggaaagg gcgtccccgc ggccctgacg ggcgccgcgg cttgcaacgc
    34921 cttcttccgt gcccggcttt ccctggcgga acaggcgggg gaatcccttc cccccgtgaa
    34981 ggacatcgtc cgcggcgcgg aggaacgtat cgccgggaag ctcatggacc tgcgcacctt
    35041 cttcaccctg gtgtacggcc gcctggacgg gaagcgggga ctgtaccgct acgtggactg
    35101 cgggcatacg agcgtcctcc atttcgagcg gaagaccggg cgctgctgga ggctcaaagg
    35161 cgcgaacgcc ccggtggggt tcctgcccga gcaggactac gtcgagtacg cggtgccgct
    35221 ctctcccggg gaccttctgt tcctgtactc cgacgggatc acggaagccg agaattccga
    35281 aggggagcgg ttcggggagg agcgcctcat gaagctcgtg ggatcccact ccgctctgga
    35341 cgccgcggcg ctcctggaca aggtcaagca catcgccttc gcctacgccg ggggggattt
    35401 caaggacgac gtcacggccc tggcggtgcg cgtcgcctcc ggcccctccg tccggaccgc
    35461 gcgccgctca ttcccccagt cgatggactc cctgcgggcg gtacgggagt tctaccgcgc
    35521 ctgcgcggac cgtgccctgt cggcctccct tccctccgag gacctggacg ccctgctcat
    35581 cgcgaccggc gaggccgccg cgaacatctt ccggcacagc gtaccggacc ggccggccga
    35641 ctgcacggcg gccttccggg tgtccgattc ctgggccgcc ttctatctct actatcgggg
    35701 ggcggattac gaatggcagg agccgaccgc cccgcccatc gagagctacc ccgcgggggg
    35761 ctacggcctg tacctcatca acgaggccat ggattccgtg accgtctcgc cgggggagga
    35821 cggggaggtt cgtctctgca tgctggtcga cctggcgtcg agggcgcgcc cttgaggggc
    35881 atccgggagt tcggggacgg gatctaccgc atagccgcgg cgggggagcc gaggttgttc
    35941 cccgggtact cgtacctggt cacggacgcg ggcaggggcg tccttttgga ccccggcccc
    36001 gcgtcggact tcgccgcgac cctggccctc tgccggtcgg tgatctcccc ggaacgcatt
    36061 tccttcctga tcctgacctc cgagaccccg gacgccgcct ccagccttcc cctgtggccc
    36121 gcttccggct tctcgggtag ggtcctggcc cactggaagg cgggactcca ggcccgatgc
    36181 tatgggggtc cggaaatccg ggacctgggg aaccatgagg aacggatctc cttcggcggc
    36241 ggcaggacct tccgcttcgt caacgtgccc ggcctgccgt ccgcgggctc gatcttctgc
    36301 ctggacgagg cctccgggag cctgttcacc ggcatggccc tgggctcggt gggagggggc
    36361 gagacggagg gggacgagga gtctcccgga aggattgagt ccttccacga cctcgtgttc
    36421 cccgccctgg gcgcccggcc ggacgtcctc gcggccctgg cttccctgtc cccccgcagg
    36481 atccttcccc ggaacggccc ccccgtcgag cggcatctgt ccgtcgcctc cgtcctgttc
    36541 gggtgcgggg acggggcggc ctgcggaccc ggggacctcc cggaagcctg cgcccgcctg
    36601 atcggggagg tgaaccagcg ccttgccgac gtgttctccc ctgcggagct ccgggacgtc
    36661 ctggccgaga ccgggctggg cttcggcgcg gacggccgcc cggagcccgc ggacctcgcg
    36721 ccctcggacc tgctggagcg gtacttcgac accctggccc gggtgaaggg tccggtgtgg
    36781 atctccctcg tgcgggccca cgtcgcccgg gggacggagg ccctggggat ccccctgccc
    36841 gccgccttcg atcggttcgg ccgggagatc gaccggggca tcggcggcct gatccgggac
    36901 atcgagagga tccggacgga gaacatcgag ctccagaaat ccatcatcca ggccagcgac
    36961 gaccacctca aggatcccgt cacggggtac tacaacgaga tcttcttcaa ggaatacctg
    37021 gacgaccttc tcctggcgga agccggcgcc gggacaccgc cgggatcggt ggccttcatc
    37081 cgtctggacg gtatccggaa gctcaacgag cggttcggga cggaggcggg cgacaccgcc
    37141 ttgaaggccc tgggcctgca cctgctcgac accaagaggc cgaactcgat cttcttccgg
    37201 atgaacggcc cgctgttcgc ctgctatctc cagggggacg gaaagacgca ggccgtggac
    37261 tacgtgcggg agctgcaggc ggaggtggag ggctcgggac gcttcgtgga gaaggtgacc
    37321 gtgtcggcgg cgatcgtgga gctttccgag atctccaccg tggccgcgga gacgggaaag
    37381 ccctacgcat gcctgatgaa ggtctgcaag gagcgcatcc accttctgga caagctcggc
    37441 ccggcctcca tctgccacga gtcctcggtg accctccgga ggaaccgggg gaacgtcctg
    37501 gtgatcgaag ccagcccctt cgaggccgac ctcctgcgga aggtcctgga acgggaaggg
    37561 ttcgaggtgc actcggtgac gagggggggc gaggccctgg cccacgcgga cctctacagg
    37621 cccgacgtga tcgtttcgga gatcttcgtc tcccagatgg acggcttcca gatacgccaa
    37681 cgcctgctcg cgtctccgga cctgaagaac atccccttca tcctggtctc ctgggacaag
    37741 ggggcctccg ccctgcgcca ggccttcgac ctgggcatcc tccatttcta caagaaaccc
    37801 gtccagccgg aggagctcat cgggatcatc aagctgctcg ccggggaggc gtccgaggag
    37861 cgccggcctt gattcttccg atatccctcc tgctattttt cctggcggcg aacggcgtca
    37921 cggtcctggc gcttctggtc gtcaagcggc gagggcgcgt gatctaccgc cgggcccaga
    37981 ccgtgcgggg cgcgctcatc tcggcggtga tcgaccggga ccggtccggg ctcgggaagc
    38041 tggcacggag gaattccggc ctttcgatcc ggctctgggc cgacctgagg gcgaccttct
    38101 acttcacgga ccgggaggcg gagcccgtgc tggaggtgtt ccgatcgtcc gggatcgagg
    38161 cgcggctcct gcggcggctc cgctcccgcg agcccctgga acgcttccgc gcctcccgca
    38221 tgctcctgcc cctggtgtct ccggggatcg tgcccgctct cgtccgggcc ctccaccggg
    38281 agacccggga tttcgtccgg atcgccctgg tcaaggatct gctgtcctcg gacctgagcc
    38341 cggcctacga cgcgatagcc gagagcctgc gggactgcgg gatggggtat ttccgttccg
    38401 tccgcgcgat gctggcggcg gtcgggaacg gcttcgtcga ctgggcacgg gatcgcctcg
    38461 tgccctcgga cccgggattc gccgtcgcgg tgctggcggg ggcccggacc caccccgccc
    38521 cgtggctccg ggatttcgtc ctcatctgcc tggaccatga cgatccccgg atccggagct
    38581 ccgccatcga ggccgcggaa tccctctacc ccgagatgct ggccggccgc gcggaggaca
    38641 ggggcgcgga catcgtgacc cgccgggcct cccttcgcgc ccaggcggag gtatcttatc
    38701 ccctggacct ggaagccttc gccgaatacc tgacggaccc gaccctccgg gacgtggcgg
    38761 cctcctgcct gtccgaatac ctcgggcgct acccggagga ggcgggccgg atcctggaac
    38821 tcctgtcccg tctgccggat tccgaggccg cccgcgccct ggcaggggtc ctgggcgtcc
    38881 ggctgccctt cctgttcgtc tcgggagagg atcccgggcg ctgtcggcgc gtcgtggaag
    38941 gcctggccgc ctcgggccgc agttcggaga tcatcgccct gctcaactcc aaccgggact
    39001 ccgagctgga ggggctcctg ctggagtgga tacggccgta tctcgggacg gttccggagt
    39061 tccgcaggga ctgcgcggga tacctggagc cccgcctgcg ggaggccctg ggcatcgccc
    39121 ccgaggaggt cccgaagagc tcccggaaga ttcccctgac ccgccgggac cggcttaggc
    39181 tggtggtcgt gctggcggcc gtggtcgccg tgccttcggc ggccatcgcc tacggccttt
    39241 ccggggccac catcggcagg acctggatcc tggcccggga gatcttccgc ctgttcacct
    39301 ggggcttcgc cttctacgcg gtcttcctga acctggtgta catcgtcctc ctgctgctgg
    39361 ccgcgcggaa cctgggcatc caggcgaagt actgggccgt cctggacaag gacttcctgt
    39421 tcaccccggg aatcatgccc tccatctcga tcctggcgcc ggcgtacaac gaggagaaga
    39481 ccgtgctgga gagcgtgcac tccctgctgt ccctccagca tcccgactac caggtcatcg
    39541 tcatcaacga tgggtcctcg gacgacacga tgggcgtcct tgcgagggcc ttcgacctgg
    39601 agcggatgga tccgagcagg accggatccc tgccgacggc ccccgtgcgg ggggtgtacc
    39661 ggtcgccggg caacccgaag ctcctggtcg tggacaagga gaacgggggc aaggcggacg
    39721 ccctgaacgc gggcatcaac ctggcccgct gcgagtacat ctgcagtatc gattccgact
    39781 ccctcctcga gcccgacgcc ctgctccgga tgaccgcgga ggtgctcacc tcggacgtcg
    39841 agaccgtggc cgtgggcggc aacatacttc ccgtgaacgg atgcaccgtg gaacggggca
    39901 tgctgcagaa gataggcctg ccatcgaatc ggatcgcccg gttccagacc atcgaatacc
    39961 tccggtcctt catagccggc cgcctgggct gggcccagat caacgccctc ctgataatct
    40021 ccggggcctt cggactcttc cggcgggacc gggtgctgga gatcgggggc tacctcacgg
    40081 ggcgggggga gttcaagcgg gacacggtgg gggaggacat ggagctggtc gtccgcctgg
    40141 tccggaggat gcgggagtcc cggcggaagt accggatacg gtacgccgcc acggcgaact
    40201 gctggaccga ctcgatgcca acctggccgt gttcggggcg g
//
LOCUS       DAOTEI010000006        40129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_661_length_40129_cov_5.791361, whole genome shotgun sequence.
ACCESSION   DAOTEI010000006 DAOTEI010000000
VERSION     DAOTEI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 40129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40129
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_661_length_40129_cov_5.791361"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="P5313_01040"
     CDS             complement(<1..393)
                     /locus_tag="P5313_01040"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HRY78976.1"
                     /translation="MLVRTKISFLTVCILAALGALSLFAARWFVLGIFDRLERESAAG
                     QALRAAALIRTSAEGLASTLGDWALWDDSYAFAGGERPGYPAENLQVETLVNLDVNLV
                     AFIGPDASRVYAKAVDLTGGVEEPVPRDV"
     gene            complement(466..1884)
                     /gene="aroA"
                     /locus_tag="P5313_01045"
     CDS             complement(466..1884)
                     /gene="aroA"
                     /locus_tag="P5313_01045"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRY78977.1"
                     /translation="MTRSVEPSNVAGVLKAPSSKSSLQRALACAALAPGVSEIAEATW
                     SADSLAACSAARALGARIERGPDGASVRGIPPAAFSDPGMPGEAPLALPCGESGTALR
                     MLTAVAALFPREIVLSGEGTLTRRPVGPVEEPLRALGAECATTGGFTPVRVRGPLRPG
                     RVRVDGSGSSQFLTGLLIALPLARTPGSGGARGGAAPGSPDAASVIEVENLVSRGYVD
                     LTLDVMRAFGVRAERSADYSEFRIPGGQTYGSASYRTEGDWSGAAFLLVAGALAARFE
                     PLEVTGLDPASSQPDRAVLDALRTAGANLELAAGSIRVRAAPLRAFSFDATDRPDLFP
                     PLAALAAFCEGTTALTGAGRLRAKESDRAAALAEELGKLGIRIEVEGDRMLVRGRGSG
                     SAGRPGGEAGTSSPGGTATGEASVVDSRGDHRIAMAAAVAALAGAGPVEIRGAECVSK
                     SYPAFFEDLAALGARVSGAVVR"
     gene            complement(1868..2395)
                     /locus_tag="P5313_01050"
     CDS             complement(1868..2395)
                     /locus_tag="P5313_01050"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRY78978.1"
                     /translation="MPAPPVPPAAASLALIGLPASGKSTVARILAGRLGIPARDLDQE
                     IADEAGRDIPGIFEAEGEAGFRDRESRALRGVAEGAPCVLAAGGGVVVRPENRELLRT
                     VFRTVWLRVSPETAAARAAGGSRPLLAGGAPEARIRDLYDRRAALYAECSILSVDTEG
                     RSPTAVAEDILDQIR"
     gene            complement(2404..2910)
                     /locus_tag="P5313_01055"
     CDS             complement(2404..2910)
                     /locus_tag="P5313_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HRY78979.1"
                     /translation="MNPEPYFLPLTSSTLVLAAGLLAALLAVVLALGLLVGRRAGRLE
                     AERGLEDRIAAEREDAVKRSRAVLGGLAAEQAAPYLPGFPADPGDLRFIGKPVDFVAF
                     AGASGGEVREVLFVEVKSGSASPSRVERSLRDAVRAGRVRWAEYRVPAPGDAPVATPE
                     LTRPASGG"
     gene            2936..4792
                     /locus_tag="P5313_01060"
     CDS             2936..4792
                     /locus_tag="P5313_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRY78980.1"
                     /translation="MPFLPVSRVDLEARGMAQADFVLVTGDAYVDHPAFAAALLGRLL
                     EAEGYSVAVLARPDPDDVEAFRLAGPPRLAFLVGAGALDSMVSSYTAARKPRSEDDYA
                     PGGRPELCLRGDGTLGPGKAGRRNARPDRAVIAYAAKCREAFKGVPVVIGGIEASLRR
                     FSHYDYWSDTMRRSVLLDAKADLLVHGMGETALLEIARRLAAGEPAPGIRGVRGTAWR
                     TSRREDLPAGAVELPPWTAVSAPGEPGFAAYAESFRLQHLNADPYSGKVLVEESEGRF
                     VVQEPPAFPLAADALDRLYEAPYERAAHPMYEGLGGVPALEEVRFSLASARGCFGSCS
                     FCSLAYHQGRIVQARTADSLEREARVLTALPGFKGYIHDVGGPTANFRAPACARQEKH
                     GACPDRRCLAPEACPSLKVDHSDYLAVLRRLRSLPGVRKVFVRSGIRFDYLMLDPDDG
                     FYRELVEHHVSGQLKVAPEHVSDRVLSLMGKPPRRVYDTFARRFAELNREYGLKQYLI
                     PYFISAHPGATLADALELAEYLRDSGFSPDQVQDFYPTPGTRSTVMFRTGRDPLTGEA
                     VYVARGERERALQRALLQYRKPENRKLVREALELLGRRDLIGRGPKCLVGER"
     gene            4949..6097
                     /locus_tag="P5313_01065"
     CDS             4949..6097
                     /locus_tag="P5313_01065"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HRY78981.1"
                     /translation="MPEAAVAAFALVFPGIALILKERVSLFREWSPVIVCYVAGLVLG
                     NLGILPDRVAGVLDGISTAAVALSIPLLLFSVDLSKWRSLAGRAGLSFVLAGVSVALV
                     SAAAVFLLNPAGGESWKLTGLLVGLYTGGTPNLAALKTALAVDQNLYLAVHASDIVLS
                     AVYLFFVMTAAKPLLGRFLPAYAAGGPESADIQPPPTRLGDLVRRGTRGPLAAAFGLA
                     VLVVAAGLGLSFLVPKDWETATVILAITTLSLAASLVPKIRNTPKTFELGEYILYVFC
                     VAVGAMGNFSRLFGAAPAVFLFTALTLFGAFLLHVLLSALFRIDVDTTLAVSVSAICS
                     PPFVGVAAVAIGNRRVIAAGITTGVIGYAVGNYLGIALAQAVRILAGG"
     gene            complement(6152..7450)
                     /locus_tag="P5313_01070"
     CDS             complement(6152..7450)
                     /locus_tag="P5313_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRY78982.1"
                     /translation="MKIFILAVAAAMYGLVIVFPHRKSVVALASAAALLAAGFLIPGE
                     GGLTLRRALLELVNWNILAIYVGSLVIADLFIYSRVPAVLADRVVEKAPTLGIAITAI
                     LVITGLVSAFVENVATVLLMAPIALAICRRMELDPAPFLIGLAVMANLQGTATLVGDP
                     PSMIFASYADYGFNDFFFKDGLPSIFFAVQAGALVGALYFFVYFRKLGKVRMDLPYER
                     IVSWVPTILLVAMIAGLALISFLSGEGLHATSGLLCLALGFAGLLWFVSIRREPASKA
                     VDLVRRLDWETILFLIGVFIVVGAIGDAGILDDVATWMSAAIGGRVFLGFAAVVAVSV
                     LISGFVDNVPYIIAMLPVAGKLASSAGMPDEVLMFGLLIGSCLGGNLTPFGASANVVA
                     VSLVKKAGRHVGFLEWVRIAGPFTVLTTIASALFVWFVWG"
     gene            7775..8116
                     /locus_tag="P5313_01075"
     CDS             7775..8116
                     /locus_tag="P5313_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78983.1"
                     /translation="MKTSDLPAAIPCAPVYDGWASKEIRGGYTSDLLSDVMSKAGEGY
                     ALITIQAHKNTVAVASMLGLAAVILCNDRPVPPDMVEAARAEGIPLFSTSETQFEVSG
                     RLWAALRGDAP"
     gene            8129..8890
                     /locus_tag="P5313_01080"
     CDS             8129..8890
                     /locus_tag="P5313_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRY78984.1"
                     /translation="MPLVRADLHNHSCLSPCGSLDLSPAVLARRARDRGLDLVALTDH
                     NSARNAPAFRACCRREGLAALYGVEATSAEEAHVLALFATVEESLAFSEFLQGHLPRL
                     PYDPRLLGDQAVVDAEDNVLDLPVLYLGAALDLGWDELCLEADSRGALVIPAHIDRLA
                     FGALAQLGFLPEGPYAAVEALHPQVRGEARGYPVISGSDAHHPEHVGRRPFVLELPED
                     WRSGDGTVRLEALREALAAGRARPYWETLPPSNGR"
     gene            complement(9074..10306)
                     /locus_tag="P5313_01085"
     CDS             complement(9074..10306)
                     /locus_tag="P5313_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY78985.1"
                     /translation="MAADRFARSKAVFERAARVIPGGIYGTKSPGFLLPGKFPYYIES
                     ASGSRITDADGNVFVDYLCGFGSQIVGYGNPAVERAGSERSARGDLLNQPSPLMADLA
                     ERLVSRIRGMDWTVFAKNGTDATTLAVSLARVHTGKRVVIAAEGAYHGAANWCSSNLF
                     PELAPDRADVALFRYNDLEGLEALFRRHRGNVACVILTPHHHPAFRPQVLPDPELYPT
                     VRRLCQAEDALFVMDDIRDGFRLHPEGSHVRFGAEPDLVCVAKALANGWPLAALLGTG
                     RVRSAADSFFITGTYWMSGAPMAAALACLDEMERMGGVGRMEALGTALREGLESAGRE
                     AGFSARVSGPAAIPYLTFDEDPDLYLNQRFCGAMADRGFFLHPHHNWFVSLAHSEADI
                     AATVEAAREVFVEMSGER"
     gene            complement(10296..11213)
                     /locus_tag="P5313_01090"
     CDS             complement(10296..11213)
                     /locus_tag="P5313_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78986.1"
                     /translation="MRPFLDDLAPAELRVLARIRLAVTARLVAAAAPLDPAVRELSAR
                     PFRLALKVPGLPRALVAGLDGRVSAWAGGEAPPLRLPADLTLLFPSARDCARVLDGVP
                     GRVLPVPGGLHAGRALRFFRAAAAAAPRILKSPETPPAVRAALLLEAAVSALVETAAA
                     DPYVAPKLEHAPDGLVVVDAGEGVRRALRKEGTRLRLVPVPGEDPGSAGAEPPDGTDP
                     AGAGRPGGGRAEAARADAVRIDAILAFRDPGAAVAVLSGKKSAVTALGACEASIRGLV
                     PLVQGLFAVLDRASRYLAVKADKEAGHGR"
     gene            11342..11767
                     /locus_tag="P5313_01095"
     CDS             11342..11767
                     /locus_tag="P5313_01095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRY78987.1"
                     /translation="MIHVTKLTVRSYECDAYGHVNNANYLHYLEVARGELLHAMGLDY
                     KGLIAAGYGLYVTKVCIEYKSPAFMEDELTIESETVTSRKLSGRMRQVVRRGDAVLAV
                     ADLDWGFVNSEGRPTKMPYEFDLTRLTAEADARAAGRTP"
     gene            11772..14645
                     /locus_tag="P5313_01100"
     CDS             11772..14645
                     /locus_tag="P5313_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRY78988.1"
                     /translation="MIGLRTAPRRGAARKTARAAAAAAFAGAAALCSFTGNSGALPPD
                     RGLAIGSLPSGLSWYLRPTERPEDRLVFRLVVAAGSARELEEERGYAHFTEHMAFNGT
                     AAYPGNTLDDLLGSLDGGLDLELNAFTYPDCTVYHLELPAALPPDRGDALALGFGILR
                     QWASELSLEPGEVEREKGVIRAELREYDTPEDRSAAREEEALYGGTPLADRGILGIPE
                     SVQAASAESLRAFYLREYLPGRMAVIAAGPFEAPEARRALTEAFADLEGRSRPVRAPA
                     LLKSDPRPGVRVIRDRDARRTRTELSLAFREPARALRTRADWAVEYRARLSERILAGR
                     LADLGADPSVPWIYLGSERREDRFGERLWRVSVSCPPGSEPEALRSLGRELGALRASG
                     GDPEEADTATLEAGLTLPYPDPDAYEAVESAIRQFLYGDASLDPGRADDLEARLRGAA
                     LAGDAPAPGVSRGDLPDLEAGILSVVRPGTGDPGDGDLASAYLEGLGEGRSVGTALRR
                     GSGRGTEPAASGVLSGTGSSASSGEGGGAGIPRARASTRVVLPDGTVDLVLANGIRVR
                     MRATGSGEGWAYLGAWSPGGLAAVPAARRAAADEVPAILARAGYPGHSTADLRAALAG
                     TWAAWRADTAFREETVEVDGGSGDLDLLFRLLAASFRPPAPDPGALRKAAAARAEELR
                     LEAGPEAEFRAVVESSDPARADPAELETWTAEAIRAAWEDRFGNPGDFEFLAVGDFDL
                     AEAERSARKHLGALPAAGIREPVPPPPPAPAPGLRGIDSGNHPRGRILVLWPLRGLSD
                     SDALRLEPLARALETRLFRRIREDLADTYDVYCVWEEWPDQPGSGEFRIELEAAPGRA
                     RALAEEVLREVAAFGAGGAGSDDAPSGFSRAADDDEAVYRKLLDGLIAERAAGGAGGR
                     GTPRRIPRNADETLEGSSPDRPKAPAARLLVPDRAAVFVLGWD"
     gene            complement(14800..15993)
                     /locus_tag="P5313_01105"
     CDS             complement(14800..15993)
                     /locus_tag="P5313_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY78989.1"
                     /translation="MKRWTAAFVLACALVSGLAAERFEHKFRAGEKYRFLSTAEEDVY
                     YNRAFSHHAEIVNRISFEVPQAFEDGSGLLRGEFTMSTRVKGLEAFVEDQRYESEYRL
                     TRNGIYDIAPRFYMPVVRNVPTFPDRDLAPGDTWAAPGEEKHDFRKEFGIPDPFVIPF
                     NAGYTYVGPETRDGIDVRVIKVSYTIFHRPPEPLIHSGIFPTQIAGWSEQTLYWDPLR
                     GGLHSYEERFQFVLELSNGTTVEFRGKAASRMLEAPPLDREALTRELQEATKDLENVT
                     VQAGDEGVTITIENVQFEADSARLLPSEQEKLGRIAEILKAYPERDILVEGHTALAGT
                     AAGRQKLSVERAAAVADRLVRLGVRTPDRIVVRGWGAERPIDTNSTEAGRSRNRRVEI
                     TILEN"
     gene            16518..17129
                     /locus_tag="P5313_01110"
     CDS             16518..17129
                     /locus_tag="P5313_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY78990.1"
                     /translation="MPAGFSERERDTIRKRLRAAALDALARGGLTAASVESLARAADI
                     AKGSFYAFYPTKEHLFLEALESIEDRYRGRFAGAVAGPGSPAERLERAFTEAFEMASS
                     EPALRRMDMRTVERLARALPPERIREHEARDARAVARIAEEWRREGLLAEGVRDAEVG
                     AAGYAVFLVAAGLGEFPEEVGGAVRSVTARGLARALAAGGGAA"
     gene            17126..17992
                     /locus_tag="P5313_01115"
     CDS             17126..17992
                     /locus_tag="P5313_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY78991.1"
                     /translation="MIEVKGLRFTYPRAKAEAVRGVDFSVAAGEVFGFLGPSGAGKST
                     TQRILLGSLGGYSGRAAVSGIEASRADGAYRSRVGAAFEFPCFYQKLTGRENLELFAS
                     FYPGPKDDPRELLERLGLAEAADRRVAEYSKGMKMRLNLIRALIHRPRVLFLDEPTSG
                     LDPANARTVRDLVRERAAAGAAVFLTTHDMAAADAACDRVAFLVDGRIAACDSPAALK
                     RRYGKRAVVVEREAPERDGPSLLSPEYPLDGLAEDEAFRAELRKPGLLGVRTLDSSLD
                     QIFLQLTGRELA"
     gene            17989..18693
                     /locus_tag="P5313_01120"
     CDS             17989..18693
                     /locus_tag="P5313_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78992.1"
                     /translation="MNLLPFIRNDFRLLWRHGFAIAYLVVALVYAAVLSAFPASWADA
                     ALPVLAWSDAAFFCFFFAGASVCLDLAQGTFRALFVSPLPPWTYFLVKTANLSVLSFG
                     MAAAVALSNRGLGFRPLPLAVAVIAGGSPSALIGAALALRLRTVNRFLVGTIPIFLVL
                     SLPILRYAAEPWIPGWLAALSRWTPTEGSFLWARAAFAPVPGAELAAGAAAALAWTAA
                     ASALILGPAVRAAKGE"
     gene            18695..19468
                     /locus_tag="P5313_01125"
     CDS             18695..19468
                     /locus_tag="P5313_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78993.1"
                     /translation="MESREPAGTDQARTEPIVGVSRNLARLAAADIRMLARDPLLAFI
                     PFIPFLAAFVLRFAVPLLSGFLERALGFRLLDWADLVRAVVLLFPGMFYGMAAGFLLL
                     DDRDDGVSAYWGVTPAGRAGYLGARLGLFSAAAFLAGVLAARALGLGRPDLGRELGAA
                     AIGAAQAAFFGLFLAAFAADKVEGLSVLKALGGLDLAPLAVLLALPLRIAAWPFPQYW
                     AAELMLGRNSRSGIALVAGLGTSALWIAALYGKYRKRIE"
     gene            19524..20942
                     /locus_tag="P5313_01130"
     CDS             19524..20942
                     /locus_tag="P5313_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78994.1"
                     /translation="MRIEPRKLGGLAAKSLGGILPAAAVLVFRPLGLDPGQTVVAAAL
                     VLAVGFWTAGAASKALVSAFLLVVFLAFGRTAPASVFRFPLSESFPVIVLAFLVSQGI
                     VKSGLARKLLLPVLDRRAGTPGRLLAAMILSSAAFVFLIPQPFSRVIILSGIFAECLE
                     ARGLPARLKERLLFALHLFSALANNLFLRGDLILNPAFLNFSGLSMGESGWTARLLVP
                     GLVHMALALVVFLLMARRDWREYARGAETSRTEGTGRPASDIPATAPASSRLSSADRR
                     NLVLIGLVLTAWALEGLHGIRGVWIAAAGTGAMFVLGLLEPKDLKAVNPGLLLFLTAA
                     SSIGAVLEGSGTAARIFPALAALLPRAWSPGLALGTVGVSMAGHMVLGSNMTTLSVLV
                     PAFRAVGEGAGVPEAVAFLVFVSVTTQFLLPFHHVVLLLGAEAGRYGRGTVFRFGLAL
                     TLVSAAAAAFLYPAWWGLRGLP"
     gene            20988..21911
                     /locus_tag="P5313_01135"
     CDS             20988..21911
                     /locus_tag="P5313_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY78995.1"
                     /translation="MDAIPINTDTGPQVVLELIYRLKVKDVMTPDPVTAAPGDTLRSI
                     QEAMRDRGFSGVPVVEAGRLVGIVSIGDILSALDGGWIGERAEGRMTRSVIVLEDDMP
                     LTFAITYHQRYRYGRFPVLDKSGKLVGILTPTDIIRSLLVEMNREVERLESRLKENAE
                     PPGGETVRLAFSTVRYDFENAGRASGAIKKALKDRGVDPAIIRRVAVASYELELNQVI
                     HSVGGTITFTLEPGRAEVRAEDRGPGIPDLDQAMREGYSTADEWIRSLGFGAGMGLPN
                     ARRSADEFTIESAPGRGTSVRVGFALKPDRA"
     gene            21920..23326
                     /locus_tag="P5313_01140"
     CDS             21920..23326
                     /locus_tag="P5313_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HRY78996.1"
                     /translation="MFDSSAYKRRREALRRSLADRGLSSGTVLILGNRESPMNYADNA
                     YPFRQDSSFLYFAGIPEPDLALTLDLETGKSVLYGEELSLDDVIWTGPRPSLRELAEA
                     AGLDGVRPRASLAGAAGSAKPLHFLPPYRAEHREELSEILGIPPKAVETKASGDLIRA
                     VIALREIKEPREVERMDAAADLSADMHRAVLARARPGVRESELAARAAEVALAGGAGL
                     AFPVIATTRGSVLHNHDHSGTLAEGDLFLMDMGAETVDGYAGDLTTTFPAGGRFDPRQ
                     REIYEILLSAFRAATDSIAPGVPYRDVHFAAAREIFTGMKALGLARGNTEEALAAGAH
                     ALFFPHGVGHQIGLDVHDLESLGEVNVGYDGQPKSPQFGLRSLRLAKPLKPGMTVTVE
                     PGIYFIAPLIAKWKAEKLHRGFLEYPKIEEWVSVGGMRNEEDWLVTDAGARKLGGPFD
                     KDAAALEAAVGSEWKGNR"
     gene            23323..24003
                     /locus_tag="P5313_01145"
     CDS             23323..24003
                     /locus_tag="P5313_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY78997.1"
                     /translation="MTGVPELTVDPSLEPRKIRVGLVWGLGLASCPPSAGPSASLAAL
                     LGQVAAAGEGFLAPERKAAVRNMLRFGAYKPAGRSKPSSEYLLAAALEGCFPIVNGPV
                     DANNEVSLRWGYPASVFDLGKTGPLLQLSRGGPGESFVFNPSGQEIDLEDLIVIRRRV
                     GDAWEACGNPVKDAMATKVFEAARDVAGVVYAPLTDPKADLEACADRFARILREDCGA
                     REAGWKIV"
     gene            24171..24707
                     /gene="purE"
                     /locus_tag="P5313_01150"
     CDS             24171..24707
                     /gene="purE"
                     /locus_tag="P5313_01150"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRY78998.1"
                     /translation="MDEPHGGSGPLVGIIMGSDSDLPVMAEASRILEELGVPHETTIV
                     SAHRTPDRMYRYAKEARERGLEVLVAGAGGAAHLPGMCASLTDLPVIGVPVRSKAFNG
                     LDSLLSIVQMPPGIPVATVAVDGGRNAGLLAARILASRRPDLAERLRAWSAARAAEVL
                     DKASRLEEEGPEALLSRI"
     gene            24710..25720
                     /locus_tag="P5313_01155"
     CDS             24710..25720
                     /locus_tag="P5313_01155"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRY78999.1"
                     /translation="MPVEIIATSDADLRRAAEILASGGLVAFPTETVYGLGGDAWNTR
                     ALARIFEAKRRPAFDPLIVHIAALAALDDVADLGALVPEARRAACDLMEEFWPGPLTL
                     ILPKRPRVPDLATSGLPTVAVRLPNHPVARALIELSTGAVAAPSANPFGYLSPTRAEH
                     VAAQLGDRVDLIVDGGRCPVGVESTVVDVTGPAPRVLRPGGLSLERLRTVAPGIEVLD
                     RTDPAPKAPGQLANHYAPRRALRLAPAGGLSAARPKGRAAALVFDAESRRRLEESAAR
                     FAEIRVLSEAGDPVEAAANLFDLLHDLDSGDCEELWAERVPEAGLGAAVNDRLYKASK
                     KT"
     gene            25790..26173
                     /locus_tag="P5313_01160"
     CDS             25790..26173
                     /locus_tag="P5313_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79000.1"
                     /translation="MQTIDSAYSLPLAVMLSRYSAPGRVSIPVPSSQVIYAHFEHVSG
                     VAAGEGRASSVDRLKILNTLIDRLSSLKSDPLTARESSGLISSDRVDALLEQYGREAH
                     ALAARSGPYAPSLGLPPGSLISLAA"
     gene            complement(26185..28512)
                     /locus_tag="P5313_01165"
     CDS             complement(26185..28512)
                     /locus_tag="P5313_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY79001.1"
                     /translation="MSRSSVSSPVKGPRHPPRAFLFYLIAAAALTGSCEDPMVLGVRA
                     HELRDRLLAGDRSLVLSAPPERLAELARLGPGALYYAARAAGLGGDPEREAVLLDLAS
                     RKEAGLFRRRAWELLPEALYRKKDWPGLLAFSERQAQAGIDDYLPRRRRVEALASLGR
                     LPETRAELDALAAAWPDRAARDEALLAGLRMRAAEDPGERAARIRELFALASPRTEDL
                     EAALGAAEAPAAAQAAAPDAATGGADPSAFSEGELALFRMRLQIARRDYGAAYRALAP
                     AQALLSPDLPRAYLSDAGKAYLYAPAPAEGIAAMTALETAAAQGSAGTPAQAGGEADP
                     REALFIAVFYRARMLARLERKAEAAAEFGRAWELAVSGEDRDAAAWYRTDGLLPGGRR
                     EAAAWLARTAPSWHNPASFADILERLAREALLAKDGATLYTLRTEVAPRMSPRAAFRL
                     VYLAGRAAEAGIAPPRASDGTAPDPGEYARQAFEEVRARSPEPYYRLLSAHRLGLPLV
                     ELPPSRPVEPRKPESRSARDLRRSGPDPEDYLAGFALYGLADLVPQEVRALEGSPDQP
                     DPETLRRLAALLRDRGYPAASMLVVNRLFETPGYEASREDWELLWPRPWPAEVSASTA
                     ASGMEEHILYGLVRSESFFRPAVVSRAGAVGLAQLMPATAEETARRLRMGSYDLENPA
                     DNLRLGSAHFAGLLRALNGRVLPSVFAYNAGLSRMRQWERAAPGFPDDLLLESLSIEE
                     TRQYGRNVLWASVVYGVLHYGLDAEGALTGMLKDR"
     gene            complement(28547..29776)
                     /locus_tag="P5313_01170"
     CDS             complement(28547..29776)
                     /locus_tag="P5313_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRY79002.1"
                     /translation="MSDRNIGIKLANGQFYPILEDGKPARKRLVVTTVKDDQPSVQID
                     MYRGSGETAASAAYIGSLVIENIPPRPKGEPDIRLELSLDEGGELLASAREEASGESQ
                     SLRVSLQALSEEEKYEIPDFEFEEESREAATTDTLPDLESAEFEVPEMAGPESPEAVP
                     KAPDLEDAGPAEDLLSEEGPEPAPEGGESYIGQSEESFTLEEEAEEASFGEPFPEDAS
                     FEEPSFEESPGGPGTVANPFEDEEPAPKPAPEPKPKKERGSGRALWIILIVLAALALA
                     LGLGLIIYRCVTAPKPAAASQPAAPAAQPAPQPAPAPAVQPAPAPAAQPAAPAPAEPP
                     KALPAGMAAYVRPEVAARPGMKPGVNYKIRWGDTLWDLSYVFYRDPWLYPRIARANKI
                     RNPNLIISGRTIWIPPR"
     gene            complement(29939..31555)
                     /locus_tag="P5313_01175"
     CDS             complement(29939..31555)
                     /locus_tag="P5313_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79003.1"
                     /translation="MPLPLLLVALYLLAVGLVGSAAARGTCRGTDPGARRDYVLGGAR
                     SGPLLLFATLAATNFSAFTVFGLSGAGYRIGWAYYPAIGFGTGLMAAAFLFLGIPLRR
                     LSARRGYLTPADFLRDRYGSPALAKAFSLCLAAVTVPYLAAQAVAGGRMISILTGIPY
                     PAASLLLVAVTALYTCRGGFRAVAATDALQLAVLAAGAAAAFAAVLGMADGSAAARLL
                     ASSPAHLSRDGAGAGVGLDRLLGLWLLWFLADPLFPQMFQRFYAARDDESLHRAAILY
                     PLICGILFFLTVGVGVAGAALLPGLPPAESEQVFPRLAAIRAEPLAGALFALAAVSAL
                     MSTLDSQLLSLSSMIVQDFAPSGARSRRTDAALVAALALLAWIASLSPPDLILDALTG
                     IAFPAYAVLAPPVWAGVYGRRANARGAAAALALGLALVVLESAGRLTFGPVPPAAVNL
                     AAQALVLAAFGRADGRREPPAPRTGILPRFRPGWLAALAATAALGTVAWEYGVPPVLV
                     GGVPRWVWLSAASCGLLALLIACWKPEPPA"
     gene            complement(31693..32457)
                     /locus_tag="P5313_01180"
     CDS             complement(31693..32457)
                     /locus_tag="P5313_01180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY79004.1"
                     /translation="MADRGTEWFEDEEFWDVFAPLMFDENRWAEVPGVVDAIERLTGA
                     SPPGAVLDACCGPGRHSLEFAGRGYRVTGVDLMDSYLEAARESARDEGLDIEFLRADL
                     REFRRPGAFDLAVNLFNSFGYFADPAQDLGMLRNIRESLKPGGRFVLEMLGKETAVRD
                     FTEGEWFERNGWTVLTEYSAVGAWEGMRNRWILIRGAERIERTFVQRLYSGTEMAALL
                     ARAGFGEVRILGSLAGAAYDRKAESLVALAFPEGSG"
     gene            complement(32459..32833)
                     /locus_tag="P5313_01185"
     CDS             complement(32459..32833)
                     /locus_tag="P5313_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79005.1"
                     /translation="MRPSDRLLSDLDDWAGRTARDPEARRFESLKALLGRSLRGIPGD
                     LLAGIAERWSALTRGPGGAARARAWLDAVCLLALMDYQDDPPLEREDWEEIRELFSAE
                     GETLDMDLLSYVLERVLDHGGF"
     gene            complement(32893..33570)
                     /locus_tag="P5313_01190"
     CDS             complement(32893..33570)
                     /locus_tag="P5313_01190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRY79006.1"
                     /translation="METPGREHPWIRAEAVLVAAVFVLPSLAAAPDAAAVETLASGSR
                     LAGNLAVLVPQTLLLLYIIGAGGRSREFHLRRPLPRDAATALVLLPLLALTVWAAASG
                     AALLPGAPPPGRAAASDLPAALFLPLLAASCLAVGYREELLFRVYLPGALEADGAPPW
                     AAAAVSIALFSAAHRYQGAAGVLGAAASGVLLTAAWGRTRSLHALAWAHAAYDAAAIL
                     AAVYGAR"
     gene            33751..34809
                     /locus_tag="P5313_01195"
     CDS             33751..34809
                     /locus_tag="P5313_01195"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY79007.1"
                     /translation="MIAVLAVVVFILFLLTITLGSRGDKRYSWFEYYAKGRESGFTFR
                     QAKDLKDLAQVAGLGDPTEVFWSLKALDRVIAAITRSRNASPKGLDRETEQLLERMYE
                     YRHRMEFDQPKYKMGIKSSHQLAANQRIRILVHGVGVFNSTLIDVHARYLVCTYPIGV
                     KLPAGFSWKGRRVSVYFWRHEDAGYVFDTYVIDDLRIRSVPVLHLAHSEALFRTQKRK
                     SVRARSRISAYMYLLKRVEGAYEKPERDPGLKCVVQDLSEDGFAVMIGGKARDGIFVK
                     IQFFLGEDQVVMSGTVKGFDYWPENNRSLVHVEAVRPSPRIRNLIRSYVYASRASDPD
                     KGGGEVFAPVHASAGRNG"
     gene            34938..36122
                     /locus_tag="P5313_01200"
     CDS             34938..36122
                     /locus_tag="P5313_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79008.1"
                     /translation="MVRILAAVLILLVSGGSALAQDTKAIIETFQRNFVRSSLGTKLE
                     LLREAAGYEGVDMGPLYETAVKFVLGNASLLESDLVMRDIAIVSAEMIRKYKYVPAAE
                     SLWDLFQTFRENSVRVPILLALGETAQDNPRLIERLNAYLGAQTTLFRSGSQPDYPTL
                     EACIEALGRLGDGSSFPVLFSAFVAGYRVDVTQKASAALAGLKGDYHGYLVQVIERNT
                     ALEKRAALDAGLSNPGFTGDQRAALAEAALSAGIGYSSGNAVETEAVREMRYKAVREL
                     TERKWQRASPAAIRHFYDVLSLFNRSQATKGSLLEAVACLGAMGTPEAAQTLSLYLQL
                     INTETEQGKVFDEQTVMAVIRNLGILGDKVAFDYLLYIGYLKYPDSIKKAAKDALQNL
                     KW"
     gene            complement(36377..36580)
                     /locus_tag="P5313_01205"
     CDS             complement(36377..36580)
                     /locus_tag="P5313_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRY79009.1"
                     /translation="MKIVLNHNPEDIPGESLTIRQILDLKGWSFPLIIARVDGTLVER
                     KDWDSVVVRDGSVVDLHHLVSGG"
     gene            36769..38658
                     /locus_tag="P5313_01210"
     CDS             36769..38658
                     /locus_tag="P5313_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY79010.1"
                     /translation="MAAAPEIPGPRRGSSLRTAILLPLASLVLLGFAASWTLYVLASR
                     ASMGGVVERLVLETTAVLDARLSRILEAPRHVGITVAEFARQVGDSESSLDLFQRVFR
                     AQLGKVPELNIAAIGFRDGEYAEAQRLGDGRILVGRAGRSTGGALESWTCDEDGNPGE
                     LYATRPGYDPRTRPWYLRSAETRIPGWSPVYSYYSNQDLAVAAGFPVLAGGEVLAVVS
                     VTTTLKGIHDFLNTMPQARRGVLAILDREGRTIAVTGKYPVSLDGDGMLPAAEESGNS
                     LLAKASPHLPLKVKPESSSLRFRLEGVRYIAGISELRNDEWDLGWRVVAILPESEFTE
                     RMTESDVRGGFVFLLLLSLALLVAWLTVDGLTEPLSQLEAAVRDLAFARFSEDSGGTV
                     RALARRRDEIGRLAASFDNMRSSLLISFETLRSNLEEKEVLLKEVHHRVKNNLQVISS
                     MLSIQAGEIEDPKALEAFAACQERILAMAFVHEGVYQSGLYTEVEMEEYLGRICSSLR
                     WSRAADAPRIDIRVDAREIRLPLSQAIPCGLIVNELTVNALKHAFPGERPGAVSVVLA
                     REDPGYRLIVEDDGVGEVPSGTSVPGIGRTLVESLVSQLRGTVTAAARPDGSGLRVDI
                     RFGTL"
     gene            38810..39268
                     /locus_tag="P5313_01215"
     CDS             38810..39268
                     /locus_tag="P5313_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY79011.1"
                     /translation="MIKYLHTDDSKCVGCMTCTAVCSQMYFKEDNPAKSCIQVFGMGK
                     GAFHLVACDQECRKCVMECPTQAITVSKTGVVMINKSLCVGCLACVAVCPIEAMRYYP
                     GHKNPFKCIACGACAKKCPKEALSVREKEESPAARRPFEVAAAVSQGGKE"
     gene            39265..>40129
                     /locus_tag="P5313_01220"
     CDS             39265..>40129
                     /locus_tag="P5313_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY79012.1"
                     /translation="MKFDAKSFKLLSEWTYEKKPLARGYNMRTLHVDVGTRKVSEKPV
                     SEDMKKRFIGGKGFGLRLLWDATKPSTTWDSPENEIVIAMGPVCGNTNYPGSGKSLVV
                     SLSPMTGVPIDSNVGGYFGPYLKFGGWDALELQGKADRETIVFLDGNEGLVRIFEAPA
                     DLPQDTHLLAEALTHAFAKDEKDLQSVSVVSAGRGADHSPMGLLNFSLYDKRRGGCRV
                     KQAGRGGIGLVLRNKKIRAVVARYKGVEQDSNGAADPAAVQRIGLKLHREIVVNDDKQ
                     CKMRKQGTAHLM"
BASE COUNT         5887 a        14029 c        14291 g         5922 t
ORIGIN      
        1 cacgtcccgt gggacgggtt cctcgacgcc cccggtcagg tcgacggcct tcgcgtagac
       61 ccgggaagcg tcgggcccga tgaacgccac gaggttcacg tcaaggttga ccagggtctc
      121 gacctggaga ttttccgccg ggtagcccgg acgttcaccg ccggcgaacg cgtacgagtc
      181 gtcccacagg gcccagtcgc ccagcgtgga ggcgagccct tccgcggatg ttcgtatcag
      241 ggcggcggcc cggagcgcct ggccggccgc ggattcccgc tcgaggcggt cgaatatccc
      301 caggacgaac catcgggccg cgaagaggga gagggcgccc agggctgcga gaatgcatac
      361 cgtcaggaag gaaattttcg tgcggacaag catacgctcc tacctcacat cctcctacgc
      421 cgcggtccgg ggcggccgcg aaccgtccgg accgatccgg acggactagc ggacgacggc
      481 gccgctcacc cgggcgccca gggcggccag gtcctcgaag aaggccgggt aggacttgga
      541 gacgcactcg gcgccccgga tttccaccgg cccggcaccg gccagggccg ccacggcggc
      601 ggccatggcg atgcggtggt ccccccggga atccacgaca gatgcctcgc cggtcgcagt
      661 cccgcccggg ctcgaggttc cggcctcccc gccgggccgg cccgcggatc cggatccccg
      721 tccccgcacg agcatccggt ctccctccac ttcgatccgg atccccagct ttccgagctc
      781 ctccgcgagg gcggccgcac ggtcgctctc cttggcccga aggcggcccg cccccgtgag
      841 ggccgtggtt ccctcgcaga aggccgccag ggctgccagg ggcgggaaga ggtccggccg
      901 gtccgtggcg tcgaaggaaa aagcccggag gggagcggcc cggacccgga tcgagccggc
      961 cgcgagctcc aggttcgcgc ccgcggtccg gagtgcgtcc aggaccgccc ggtcgggctg
     1021 ggaggaggcg gggtcgagtc ccgtgacctc caaaggctca aaccgggccg ccagggcacc
     1081 ggccaccagg aggaaggcgg ccccgctcca gtccccctcg gtccggtacg acgcggaacc
     1141 gtaggtctgg cccccgggaa tacggaactc cgagtagtcc gcgctgcgtt cggcccggac
     1201 cccgaaggcc cgcatcacgt ccagggtcag gtccacgtaa cctcggctca cgaggttctc
     1261 gacctcgatc acggaggctg cgtccgggga ccccggggcg gcgccgcccc gggccccgcc
     1321 ggatcccggc gtccgcgcca ggggcagggc gatgagcaaa cccgtgagga actgggagct
     1381 gccgctcccg tccacccgga cccggcccgg acggaggggg ccccgcaccc gaaccggagt
     1441 gaagcctccc gttgtggcgc attccgcccc cagggcccgc aggggttcct cgaccgggcc
     1501 gacgggacgc cgggtcaggg tgccttcgcc ggaaaggacg atctcccggg ggaagagggc
     1561 cgccacggcg gtgagcatcc ggagggccgt gccggactcc ccgcagggga gagcgagcgg
     1621 cgcttcgccc ggcatccccg ggtccgagaa ggccgcgggc gggatgcccc ggaccgaggc
     1681 gccgtccggg ccgcgctcga tccgggcacc cagggcccgg gccgcggagc aggccgcgag
     1741 ggagtccgcg gaccaggtgg cctccgcgat ctccgagaca cccggcgcca gggccgcgca
     1801 ggccagggcc cgctggaggg agctcttgga ggagggcgcc ttcaggacgc cggccacgtt
     1861 cgagggctca acggatctgg tcaaggatgt cctccgccac agccgtcggg ctccggccct
     1921 cggtgtccac ggatagaatc gagcattcgg cgtagagggc cgcccgtcgg tcgtacaggt
     1981 cccggatccg ggcctccggg gcgcccccgg ccaggagggg gcgcgagccc cccgcggccc
     2041 gggccgccgc cgtttccggg gagacccgga gccagaccgt ccgaaacacc gtccgcagga
     2101 gctctcggtt ttcgggccgg accacgacac cgccccccgc cgcgaggacg cagggggctc
     2161 cctccgcgac tccccgaagg gcccggctct cccgatcccg gaagcccgcc tccccctcgg
     2221 cctcgaagat cccggggatg tcccgtcccg cctcgtccgc gatctcctgg tccaaatccc
     2281 gggcggggat gcccaggcgg ccggccagga tccgggccac cgtggatttc cccgaggccg
     2341 ggaggccgat cagggcgagg gaggccgctg cggggggtac gggaggcgcg ggcatgggga
     2401 atcctagccg ccggaggcgg gccgggtcaa ttccggtgtg gcgaccggcg cgtcacccgg
     2461 cgccggaacc cggtactccg cccagcggac ccggccggcc cgcaccgcgt cccggaggct
     2521 gcgctcgacc cggctgggag aggcggaacc ggacttcacc tccacgaaga ggacctcccg
     2581 gacctccccc ccggaggccc cggcgaaggc cacgaagtcc acgggcttgc cgatgaaccg
     2641 gaggtccccg gggtcggcgg gaaagccggg caggtagggg gccgcctgct ccgccgcgag
     2701 gccccccagg accgcccggg accgcttcac cgcgtcctcc cgctccgcgg cgatgcgatc
     2761 ttcaaggccc cgttccgcct ccaggcggcc ggcgcgccgt ccgaccagga ggccaagggc
     2821 caggacgacc gccagcaggg ccgccaggag tcccgcggcc aggaccaggg tggaactcgt
     2881 caggggcagg aaataaggtt ccgggttcat ggggggagta tagtatactc ctcccatgcc
     2941 cttcctcccc gtgtcccggg ttgacctgga agcccggggg atggcccaag ccgacttcgt
     3001 cctcgtcacc ggggacgcct acgtggacca ccccgccttc gccgccgccc tcctggggcg
     3061 tctgctggag gccgaaggct actccgtcgc ggtcctggcc cggcccgacc cggacgacgt
     3121 cgaagccttc cgcctggcgg ggcctccccg gctggccttc ctggtcgggg cgggggccct
     3181 ggactccatg gtctcctcgt acacggcggc ccggaaaccc cggtccgagg acgactatgc
     3241 cccgggaggc cggccggagc tctgtctccg gggggatggc accctgggcc ccgggaaggc
     3301 ggggcggcgc aacgcccggc cggaccgggc ggtcatcgcc tacgcggcca agtgccggga
     3361 ggccttcaag ggcgtgcccg tggtgatcgg gggcatcgag gcgtccctgc gacgtttttc
     3421 ccactacgac tactggtccg acacgatgcg gaggtccgtg ctcctggacg ccaaggcgga
     3481 cctcctggtc cacggcatgg gggagacggc cctcctcgag atcgcacgcc gcctggccgc
     3541 cggagagccc gcccccggga tccggggcgt ccgggggacc gcatggagaa cctctaggag
     3601 ggaggatctg cccgccgggg ccgtcgagct gccgccctgg accgccgtct ccgccccggg
     3661 ggagccgggg ttcgcggcct acgccgagtc cttccgccta cagcacctca acgcggaccc
     3721 gtactcgggg aaagttctcg tggaggagtc cgagggccgc ttcgtggtcc aggagcctcc
     3781 cgccttcccc ctggccgcgg atgccctgga ccgcctgtac gaggccccct acgagcgggc
     3841 agcccatccg atgtacgagg ggctcggggg cgttcctgcc ctggaggagg tccgcttctc
     3901 cctggcctcg gcccggggct gcttcggatc ctgctccttc tgctccctgg cctaccacca
     3961 gggcaggatc gtccaggccc ggaccgcgga ctccctggag cgggaggccc gggtcctgac
     4021 ggccctgccg ggattcaagg gctacatcca cgacgtgggc ggccccaccg ccaacttccg
     4081 ggcccccgct tgcgcccgcc aggaaaagca cggggcctgc ccggaccggc gctgcctggc
     4141 ccccgaggcc tgcccctccc tcaaggtcga ccattcggac tatctcgccg tgctccgtcg
     4201 cctccgatcc ctgccggggg tcaggaaggt cttcgtccgg tccgggatcc gtttcgacta
     4261 cttgatgttg gaccccgacg acggcttcta tcgggagctc gtggagcacc acgtctccgg
     4321 ccagctcaag gtagccccgg agcacgtctc ggaccgggtc ctttccctca tgggcaaacc
     4381 ccccaggagg gtctacgaca ccttcgcccg gcgcttcgcc gagttgaacc gcgagtacgg
     4441 cctcaaacag tacctgatcc cctacttcat ctccgcccac cccggggcga ccctggcgga
     4501 tgccctggag ctggcggaat accttcggga ctcggggttc tcaccggacc aggtccagga
     4561 cttctacccg accccgggga cacggtccac ggtcatgttc cggaccggcc gggatcctct
     4621 cacgggcgag gcggtctacg tggcccgggg cgagcgggag cgggccctcc agagggccct
     4681 cctccagtac cggaagcccg agaaccggaa gctcgtgcgg gaggccctgg agctcctggg
     4741 ccgccgggac ctgatcggga gagggccgaa gtgtctggtc ggtgagcggt gaaccgtgga
     4801 cggtaaacgg aaatgagact accgcggcgg acaccttgaa cgccgaagcg tagtcaagta
     4861 tacgcagtaa aggctcactt ccgtccaccg accaccgttt acagtccact gtccctcagg
     4921 ctatccttcc cccccaagga gtccccccat gcccgaagcc gccgtagccg ccttcgccct
     4981 cgtattcccc ggcatcgccc tgatcctgaa ggagagggtg tccctcttcc gggaatggag
     5041 ccccgtgatc gtctgctacg tcgcgggcct ggtcctgggg aacctgggca tcctgcccga
     5101 ccgggtcgcg ggggtcctgg acgggatctc caccgccgcc gtggccctct ccattcccct
     5161 cctcctgttc tccgtggacc tctccaagtg gaggagcctg gcgggccggg cggggctgtc
     5221 cttcgtcctg gcgggtgtct ccgtggccct ggtgtccgcg gccgccgtct tcctgttgaa
     5281 tcccgcgggc ggcgagtcct ggaagctaac gggcctcctg gtgggcctgt ataccggcgg
     5341 gactcccaac ctggcggccc tcaagacggc cctggcggtg gaccagaatc tctatctagc
     5401 ggtccacgcc tcggacatcg tcctgtccgc ggtctacctc ttcttcgtga tgaccgccgc
     5461 caagcctctc ctggggcgtt tcctgcccgc ctatgccgcg gggggcccgg agtcggcgga
     5521 cattcagccg cccccgacgc gcctgggcga cctggtccgg cgcggaaccc ggggacccct
     5581 ggccgcggcc ttcgggctgg cggtcctggt ggtcgcggcg gggctcgggc tgtccttcct
     5641 ggtacccaag gactgggaga ccgcgacggt gatcctcgcc atcaccaccc tgagcctggc
     5701 cgcctccctc gtcccgaaga tccggaacac gccgaagacc ttcgaactcg gggagtacat
     5761 cctgtacgtc ttctgcgtgg ccgtgggggc catgggcaac ttctcccggc tcttcggggc
     5821 ggcacccgcg gtgttcctct tcaccgccct caccctgttc ggcgccttcc tgctccacgt
     5881 gctcctgtcc gccctgttcc gcatcgacgt ggacacgacc ctggcggtct ccgtgtccgc
     5941 gatatgctcg ccgcccttcg tgggcgtcgc ggcggtggcc atcgggaacc ggcgggtcat
     6001 cgccgcgggg atcacgacgg gggtcatcgg ctacgccgtg ggcaactacc tgggcatagc
     6061 cctggcccag gcggtgcgga tcctcgcggg agggtaggcg gagtagcgga cggccgcgga
     6121 ttccggaccc gcggcgtatc cggggccggc ctcagcccca gacgaaccag acgaagaggg
     6181 ccgaggcgat cgtggtgagc accgtgaagg ggcccgcgat ccggacccat tccaggaagc
     6241 ccacgtgccg gcccgccttc ttcaccaggc tcacggccac gacgttggcg gaggccccga
     6301 agggggtcag gttgcccccc aggcaggacc cgatcaggag cccgaacatc agtacctcgt
     6361 cgggcatccc cgcggaggag gccagcttcc cggccacggg gagcatggcg atgatgtagg
     6421 gcacgttgtc cacgaagccc gagatcagga ccgagacggc cacgacggcg gcgaacccca
     6481 ggaagacgcg gcctccgatc gcggcgctca tccaggttgc gacgtcgtcc aggatgccag
     6541 cgtccccgat ggctcccacc acgatgaaga ccccgatcag gaagaggatg gtctcccagt
     6601 ccaggcgcct caccaggtcc acggccttgg aggccggctc cctccggatg gacacgaacc
     6661 agagcaggcc cgcgaagccg agggccaggc agaggagccc cgaggtcgcg tggaggccct
     6721 ccccggagag gaaggagatc agggccagtc ccgcgatcat cgcgaccagg aggatcgtgg
     6781 ggacccagga gacgatgcgc tcgtagggga ggtccatccg gaccttcccg agcttccgga
     6841 agtacacgaa gaagtagagc gcacccacga gggcccccgc ctggacggcg aagaagatgg
     6901 acggcaggcc gtccttgaag aagaagtcgt tgaatccgta atcggcatag ctggcgaaga
     6961 tcatggaggg gggatccccg acgagggtcg cggtgccctg gaggttggcc atcaccgcga
     7021 gtccgatcag gaagggggcg ggatccagct ccatccggcg gcagatggca agggcgatgg
     7081 gggccatgag gaggacggtg gcaacgttct ccacgaaggc ggagaccagg cccgtgatca
     7141 ccaggatggc ggtgatggcg atccccaggg tcggggcctt ctccaccacc cggtccgcca
     7201 ggaccgcggg gacccgggag tagatgaaga ggtccgcgat caccaggctt ccgacgtaga
     7261 tggccaggat gttccagttc accagctcca gcagggcgcg gcggagggtc agtccgcctt
     7321 ccccgggaat caggaatccc gccgccagga gggcggcggc agaggccagg gcgacgacgg
     7381 atttgcggtg gggaaacacg atgaccaggc cgtacatggc ggcggccacg gccaggatga
     7441 agatcttcac ggaggcatcc tcctcgcgaa ggctccgctc cggctcggga acggacccgt
     7501 actcgacgcg ccgaaacggg cgcgcccgta cggtcttgcc ccgtcccgtt tccgggactc
     7561 gaaccgccgg gagccgggaa ggctccgtcc tgacggcggg ttcttccgcc ggacccgcgg
     7621 cccggcggcg ggctactccc cgtccgatgg aaggaatcta ccacggccta ggcggccgga
     7681 caaggagggc ggcccccgcc tccggggctt ccctcggccg ccggttttcc gtacaataga
     7741 taaaaattta cgcattcgcc cgacggaggc aaccatgaag acgtccgacc tgcccgccgc
     7801 gatcccctgc gctcccgtgt acgacggatg ggcgtccaag gagattcggg ggggctatac
     7861 ctccgatctg ctgtccgacg tcatgagcaa ggccggggaa gggtacgcgc tcatcacgat
     7921 ccaggcccac aagaacaccg tggccgtggc ctccatgctg gggctggcgg cggtgatcct
     7981 ctgcaacgac cggcccgtac ccccggacat ggtcgaggcc gcccgggcgg agggcatccc
     8041 cctcttttcg acctccgaga cccagttcga ggtctccggc cgtctttggg ccgccctgcg
     8101 cggggacgcg ccgtagcgga ggcgggcggt gcccctcgtc cgggcggacc tgcacaacca
     8161 ctcctgcctt tccccctgcg ggagcctgga tctgagcccc gccgtcctgg cccgccgggc
     8221 ccgggaccgg ggtctggacc tggtggccct cacggaccac aactccgccc ggaacgcccc
     8281 ggccttccgg gcgtgctgcc gccgggaggg tttggccgcc ctctacggcg tggaggcgac
     8341 cagcgccgag gaggcccacg tcctcgctct cttcgcaacg gtggaggagt ccctcgcttt
     8401 ttcggaattc ctgcaggggc acctgccccg tctgccctac gatccccgct tactggggga
     8461 tcaggccgtg gtggacgcgg aggacaacgt cctggacctc ccggtgctct acctcggggc
     8521 ggccctggac ctgggctggg acgagctctg cctcgaggcg gacagccggg gcgccctggt
     8581 gatccccgcc cacatcgacc gcctcgcgtt cggggccctg gcccagctgg gcttcctccc
     8641 cgaggggccc tacgccgccg tggaggccct gcatccgcag gtccgcggcg aggcgcgggg
     8701 ctacccggtg atctcgggat ccgacgccca ccaccccgag cacgtcgggc gccggccctt
     8761 cgtcctcgaa ctccccgagg attggcgctc cggggacggt accgttcgac ttgaagccct
     8821 gcgggaggcc ctggccgcgg gccgtgcccg gccctactgg gagacgcttc cgccgtctaa
     8881 tggtcgatag acttttatat ccaatagcac atatcaggaa ctcaccacgg gcatacatca
     8941 ggagcgattt ggaaccctag atccaacagg atgaacatga taggacaaga ttaacaggat
     9001 gggagatgga attgaggatc aacgtatcct ttgatcttgt tcatccttct tcatcttgtc
     9061 catcttgttg gaattatcgt tcaccgctca tctccacgaa gacctcccgg gcggcctcga
     9121 cggtggccgc gatgtccgcc tccgagtggg ccagggatac gaaccagttg tggtggggat
     9181 gcaggaagaa accccggtcc gccatggctc cgcagaagcg ctggttcagg tagaggtcgg
     9241 ggtcttcgtc gaaggtgagg taggggatgg ccgcaggtcc ggagacccgg gcgctgaagc
     9301 ccgcctcccg ccccgccgat tccagtccct cccgcagggc cgtgcccagg gcctccatgc
     9361 gtccgacgcc gcccatgcgt tccatctcgt ccaggcaggc cagggcggcc gccatggggg
     9421 ccccggacat ccagtaggtg ccggtgatga agaaggagtc cgccgcggat cgaacccgcc
     9481 cggtgcccag gagggccgcc aggggccagc cgttggccag ggccttggcc acgcagacca
     9541 ggtcgggctc ggcgccgaag cggacgtggc ttccctcggg gtggaggcgg aatccgtccc
     9601 ggatgtcgtc catcacgaag agggcgtcct cggcctggca gaggcggcga accgtggggt
     9661 agagctcggg atccggcagg acctgggggc ggaaggcggg gtggtggtgg ggggtcagga
     9721 tcacgcaggc cacgttcccc cggtgccgcc ggaagagggc ttccagaccc tccaggtcgt
     9781 tgtaccggaa tagggccacg tccgcccggt ccggggccag ctctgggaag aggttcgagg
     9841 agcaccagtt ggccgccccg tggtaggccc cctccgcggc gatcacgacc cgtttgccgg
     9901 tgtgcacccg ggccagggag accgccaggg tggtggcgtc ggtgccgttc ttggcgaaaa
     9961 ccgtccagtc catgccccgg atccgggaga ccaggcgctc cgcgaggtcc gccatcaggg
    10021 gggagggctg gttgaggagg tcgccccggg cggagcgctc ggacccggcc ctctccaccg
    10081 cggggttgcc gtagcccacg atctggctcc cgaagccgca gaggtagtcg acgaagacgt
    10141 tcccgtcggc gtccgtgatc cgggaccccg aggcgctctc gatgtagtag gggaacttgc
    10201 cgggcaggag gaagcccggg ctcttggtcc cgtagatgcc gccggggatg acccgggcgg
    10261 cccgctcgaa gacggccttg gatcgtgcga agcggtcagc ggccatgtcc ggcctccttg
    10321 tcggccttga cggccaggta acgcgaagcc cggtccagga ccgcgaagag cccctgcacc
    10381 aggggcacga gaccccggat cgaggcctcg caggccccca gggcggtgac ggcggacttt
    10441 ttcccggaca ggacggccac cgccgccccc ggatcccgga aggcgaggat cgcgtcgatt
    10501 cggacggcat ccgcccgggc ggcttccgcc cggcctccgc cgggccggcc cgcgcccgcc
    10561 ggatccgtcc cgtccggcgg ctcggccccg gcggatccgg ggtcctctcc gggaaccggc
    10621 accagccgga gccgggtgcc ctccttccga agagcccggc gtaccccctc cccggcatcc
    10681 acgaccacga ggccgtccgg ggcgtgctcc aacttcgggg ctacgtaggg gtccgcggcg
    10741 gcggtctcga ccagggcgga caccgccgct tccaaaagga gggccgcccg taccgccggc
    10801 ggggtttccg gggatttgag gatccggggg gcggcggccg cggcggcccg gaagaaccgg
    10861 agggcccggc ccgcgtgcaa gccgcccggg acggggagga ccctgcccgg gaccccgtcc
    10921 aggacccggg cgcagtcccg ggccgagggg aagagtaggg tgaggtccgc gggcagacgg
    10981 aggggcgggg cctcccctcc ggcccaggcc gagactcggc cgtccaggcc ggccaccagg
    11041 gcgcggggaa ggccgggaac ctttagggcc aggcggaagg ggcgggcgga gagttcccgg
    11101 acggcggggt ccaggggggc cgcggcggcc acgagtcggg cggtcaccgc caggcggatc
    11161 cgggccagga cccggagctc ggcgggagcg agatcgtcca ggaagggacg catggtgcct
    11221 ccggccgccg cgggaaggat gcgggaaagt cgcccgggct cccctcggta cggggtccgg
    11281 cgcgggccgc ggcgttccgg ccactgtagc ggaaacggcc cgattccgcc atactcgccc
    11341 catgatccac gtcacgaaac tgaccgttcg gtcctacgag tgcgacgcct acgggcacgt
    11401 gaacaacgcc aactacctcc actacctgga ggtggcccgg ggcgagctcc tccatgccat
    11461 ggggctggat tacaagggtc tcatcgccgc ggggtacgga ctctacgtca ccaaggtctg
    11521 catcgagtac aagtccccgg ccttcatgga ggacgagttg acgattgaat ccgagaccgt
    11581 cacgtcccgg aagctgagcg gccgtatgcg ccaggtcgta cgccggggag acgccgtcct
    11641 ggccgtcgcg gacctggact gggggttcgt caatagcgag ggccggccca ccaagatgcc
    11701 ctacgagttc gacctgaccc gcctgaccgc ggaggcggac gcccgggccg cgggccggac
    11761 tccctgatgc cgtgatcggg cttcgaaccg ccccccgccg gggcgccgcc cgaaaaaccg
    11821 cccgtgcggc ggccgcggcg gcgttcgccg gtgccgcggc cctgtgctcc ttcaccggga
    11881 actccggcgc cctccccccg gaccggggcc tggcgatagg ctccctcccc tcggggctgt
    11941 cctggtacct gcgcccgacg gagcggcccg aggatcgcct ggtgttccgg ctggtcgtag
    12001 cggcgggctc cgcccgggaa ctcgaggagg agcggggcta cgcccatttc accgagcaca
    12061 tggccttcaa cgggaccgcg gcctaccccg ggaataccct ggacgacctc ctgggctccc
    12121 tggacggggg cctcgacctg gagctcaacg ccttcaccta cccggactgc acggtctacc
    12181 acctggagct cccggccgca ctccccccgg accgcgggga cgccctggcc ctgggcttcg
    12241 gaatcctgcg gcagtgggcc tcggaactga gcctcgaacc gggggaggtc gaacgggaga
    12301 aaggcgtcat ccgggcggaa ctgcgggagt acgacacccc tgaggacaga tcggcggcga
    12361 gggaggagga agccctgtac gggggcacac ccctggcgga ccggggcatc ctgggcatcc
    12421 cggagtcggt gcaggcggcc tcggcggagt ccctccgggc cttctacctc cgggagtacc
    12481 ttcccggccg aatggccgtg atcgccgcgg gccccttcga ggccccggag gcgcggcggg
    12541 ccctgacgga agcctttgcg gacctcgaag gccggtcccg gcccgtccgg gcgccggccc
    12601 tcctgaagtc cgatccgcgg ccgggcgtcc gggtgatccg ggaccgggac gcccgccgga
    12661 cccggacgga gctgagcctc gcgttccggg aacccgcccg ggccctccgg accagggcgg
    12721 actgggcggt cgagtaccgg gcccgccttt cggagaggat cctggcgggg cgcctggcgg
    12781 acctgggggc cgacccctcg gtcccgtgga tctacctggg ttcggagcgc cgggaggacc
    12841 ggttcgggga gcgcctgtgg cgggtgtccg tgtcctgtcc gcccgggtcc gagccggagg
    12901 ccctgcgttc cctgggccgg gagctcgggg ccctgcgggc ctccggagga gatcccgagg
    12961 aagcggatac cgcgaccctc gaggccggcc tgacccttcc gtacccggat cccgacgcct
    13021 acgaggcggt cgagtccgcg atccggcagt tcctctatgg agacgcctcc ctggacccgg
    13081 gccgggcgga cgacctggag gcccggctgc gaggagccgc cctggcgggc gatgccccgg
    13141 ctccgggcgt ttcccggggc gatcttccgg atctcgaggc ggggatcctg tccgtggtcc
    13201 gtcccgggac gggagacccc ggggacgggg atctggcctc ggcctacctg gaggggctcg
    13261 gcgaggggcg gagcgtcggg accgccctcc ggcggggttc cgggcgcggc accgagccgg
    13321 ccgcgtcggg ggtgctttcc gggaccgggt cttccgcgag ctccggagag gggggcggtg
    13381 ccggaatccc gcgggcccgg gcctcgaccc gggtagtgct tcccgacgga acggtcgacc
    13441 tggtcctggc gaacgggatc cgggttcgga tgcgggctac cggctccggc gagggctggg
    13501 cctacctggg cgcctggtcc cccggggggc tggccgccgt tcccgccgcc cgccgggccg
    13561 ccgcggacga ggtgcccgcg atcctggccc gggcggggta tccgggacac tcgaccgcgg
    13621 acctgcgggc cgcgttggcg ggcacctggg cagcctggcg ggccgacacg gcctttcggg
    13681 aggaaacggt cgaggtcgac ggcggttccg gggacctgga tctcctcttc cgcctgctgg
    13741 ccgcctcttt ccggcccccc gcaccggatc ccggcgccct ccgcaaggcc gcggcggccc
    13801 gggccgagga gctccggctg gaggccggcc ccgaggccga gttccgggcc gtcgtggagt
    13861 cgtccgatcc cgcccgcgcg gatcccgcgg agctcgagac ctggacggcc gaggcgatcc
    13921 gggccgcctg ggaggaccgc ttcggaaatc ccggggactt cgagttcctg gcggtcgggg
    13981 acttcgacct cgccgaagcc gagcgctcgg cccggaagca cctgggagcc ctcccggcag
    14041 ccggaatccg ggagcccgtc ccgccgccgc ctcccgcccc tgcgccgggc ctgcgcggga
    14101 tcgattccgg gaaccatccc cggggccgga tactcgtcct gtggcctctc cggggcctct
    14161 cggattccga cgccctccga ctggaacccc tggctcgcgc cctggagacc cggctcttcc
    14221 gccgtatccg ggaagatctc gcggacacct acgacgtgta ctgcgtctgg gaggagtggc
    14281 cggaccaacc cggctccggg gagttccgga tcgagctaga ggcggcaccc ggccgggccc
    14341 gggccctggc ggaggaggtc ctccgggagg tggccgcctt cggtgccggg ggtgccggct
    14401 cggacgacgc tccctccgga ttctcccggg cggccgacga cgacgaggcg gtgtaccgga
    14461 aactcctgga cgggctgatc gccgagcggg cggccggcgg cgcgggagga cggggcacgc
    14521 cgcggcgtat cccccggaat gcggatgaga ccctcgaagg ctcctccccg gaccggccga
    14581 aggcccctgc cgcacgcctc ctggtcccgg atagggccgc tgttttcgtc ctcggctggg
    14641 actgaggcct agcaggttgt tgaaatactc gaagcatttc aacaacctgc tatgcaattg
    14701 ctcgagaacc ttgcggttct ctgcgcaatt gcgacattaa ggtagtgttg aaaaaggctc
    14761 tgtagccttt ttcaacaccc tgctaggccg ccgccgggat caattttcca ggatggtgat
    14821 ctccacccgg cggttccggg acctccccgc ctcggtggaa ttcgtgtcga tgggccgttc
    14881 cgcgccccag ccccggacta cgatgcgatc cggggtgcgg acccccaggc ggacgagtcg
    14941 gtccgccacg gcggcggccc gctccaccga gagcttctga cgacccgcgg cggtgcccgc
    15001 cagggcggtg tgcccctcca ccaggatgtc ccgctcggga taggccttca ggatctccgc
    15061 gatccgcccg agcttctcct gttccgaagg caggaggcgc gcggagtccg cctcgaactg
    15121 gacgttctcg atggtgatgg tcacaccctc gtccccggcc tggaccgtca cgttttccag
    15181 gtccttcgtg gcctcctgga gctcccgggt cagagcctcg cggtcgaggg gcggggcctc
    15241 cagcatccgg gaggcggcct tcccgcggaa ttcgacggtg gtcccgttgg acagttccaa
    15301 gacgaactgg aagcgctcct cataggagtg gagtcccccg cgcagggggt cccagtagag
    15361 ggtctgctcg gaccagcccg ctatctgggt ggggaagatt ccggagtgga tcaggggttc
    15421 cggggggcgg tggaagatgg tgtaggagac cttgatcacc cgtacgtcga tcccgtcccg
    15481 ggtctccggg ccgacgtagg tgtagccggc gttgaagggg atgacgaagg gatcggggat
    15541 cccgaattct ttgcggaagt cgtgcttctc ctccccggga gcggcccagg tgtccccggg
    15601 ggccaggtcc cggtccggga acgtgggaac gttccggacc acgggcatgt agaaccgggg
    15661 ggcgatgtca tagatcccgt tccgggtcag gcggtattcg gattcgtagc gctggtcctc
    15721 gacgaaggct tccagaccct tcacccgggt cgacatggtg aactctcccc gcaggaggcc
    15781 cgagccgtcc tcgaacgcct gcgggacctc gaaggaaatc ctgttcacga tctccgcatg
    15841 gtgggagaat gcccggttgt agtagacatc ctcctcggcg gtggagagga agcggtactt
    15901 ctccccggca cggaacttgt gctcgaaccg ttccgccgcc agtccggaca cgagcgcgca
    15961 ggcgagaacg aaggccgcgg tccaccgttt catggctacc cccaagacta gtatcggtca
    16021 tcctggaaac tactggcaac agggagggtt tcaaatccca agggattcct aggagcctgt
    16081 cggacttgtg gtttttagcg ccgataggcg caaaaaaacc cgattatccg gctcctttgg
    16141 agtttgcaag gtagaaaaat cgcacaaatg cgattttcta ttattttcgt gggcaagccc
    16201 gacaaactcc taggcgggtc taggggcgga tccggagctt gccgggccgc acttcgtata
    16261 tatcacgggg tcggcgtgtt atctgaagtt cgcataggtg gtcccgggcc ggcagtatgt
    16321 gtagttcgca tatcgtgcac gagaccggcc gggacccggg atcggccgcg gccccgccgt
    16381 gccatccgct cccccttgac gggaatccat gtatgattta ccatatgacc agattggtat
    16441 aaatggtcat atgcgcgcac gatcaccccg ggccgttccc ggcgaatcgg gcggggtcga
    16501 tggaacaagg aggccgcatg ccggcgggat tcagcgagag ggaacgggat acgatccgga
    16561 agcggctccg ggccgcggcg ttggacgccc tggcccgggg gggcctgacc gcggccagcg
    16621 tggaaagcct ggctcgggcc gcggacatcg cgaagggttc cttttacgcc ttctacccga
    16681 caaaggaaca cctcttcctg gaggccctgg aatccatcga ggaccgatac cgtgggcggt
    16741 tcgcgggcgc cgtggcgggc ccgggttccc cggcggagcg actggagagg gctttcacgg
    16801 aagccttcga gatggcctcc tcggagcccg cgcttcgacg catggacatg cggaccgtgg
    16861 aacgcctggc ccgggccctg cccccggagc ggatccggga gcatgaggcc cgggacgccc
    16921 gggcggtcgc ccggatcgcc gaggagtggc ggcgggaggg gctcctggcc gagggtgtcc
    16981 gggacgcgga ggtcggcgcc gcgggctacg cggtattcct tgtggccgcg ggcctcgggg
    17041 agttcccgga ggaagtgggc ggcgccgtgc ggagcgtgac ggcccggggc ctggcccggg
    17101 ccctggcggc agggggaggt gcggcatgat cgaggtgaag ggtctgcggt tcacataccc
    17161 ccgggccaag gccgaggcgg tccggggcgt agatttctcc gtggccgccg gggaggtttt
    17221 cgggttcctg ggccccagcg gggcggggaa gagcaccacc cagaggatac tcctgggatc
    17281 cctgggggga tactccgggc gggcggccgt gtcggggatc gaggcctccc gggcggacgg
    17341 ggcctaccgg tcccgggtgg gggcggcctt cgagtttccc tgtttctacc agaagctgac
    17401 gggccgggag aacctcgagc tcttcgcctc cttctatccg gggcccaagg acgaccctag
    17461 ggaactcctg gaacgcctgg ggctggcgga ggccgcggac cggagggtgg cggaatactc
    17521 caaggggatg aagatgcgcc tgaacctgat ccgggccttg atccaccgcc cccgggtcct
    17581 cttcctggac gagcccacga gcggcctgga ccccgccaac gcccggacgg tccgggatct
    17641 cgtccgggag cgcgccgccg ccggagccgc ggtcttcctg accacccacg acatggccgc
    17701 cgcggatgcc gcctgcgacc gggtggcctt cctggtggac gggcggatcg cggcctgcga
    17761 ctccccggcc gccctgaagc gccggtacgg gaaacgcgcc gtggtcgtgg agcgggaagc
    17821 tccggagcgg gacgggccct ccctcctctc cccggagtac cccctggacg gcctggcgga
    17881 ggacgaagcc ttccgggcgg agctgaggaa acccggcctc ctgggggtcc gcaccctgga
    17941 ttccagcttg gaccaaatat tcctccagct gacgggaagg gagctggcat gaatctcctt
    18001 cccttcatcc gtaacgattt ccgcctcctg tggcggcacg gcttcgccat cgcgtacctg
    18061 gtcgtcgccc tggtctacgc ggccgtcctc tcggcgttcc cggcatcctg ggcggacgcg
    18121 gccctgccgg tcctggcctg gagcgacgcg gccttcttct gtttcttctt cgcgggcgcc
    18181 agcgtctgcc tggacctggc acaggggacc ttccgggccc tcttcgtctc ccccctgccg
    18241 ccctggacct acttcctggt caagaccgcg aatctctcgg tcctgtcctt cggcatggca
    18301 gcggcggtgg ccctgtccaa ccggggcctc gggttccggc ccctgcccct ggcggtcgcg
    18361 gtcatagccg gaggctcgcc ttccgccctg atcggggcgg ccctggccct gcggctccgg
    18421 accgtgaatc gcttcctggt ggggaccatc ccgatcttcc tggtcctgtc cctgccgatc
    18481 ctccgctacg ccgcggagcc ctggataccg gggtggctgg ccgccctgtc ccgatggacc
    18541 ccgacggagg gatcctttct ctgggctcgg gcggcgttcg cccccgttcc cggagccgaa
    18601 ctggccgcgg gcgcggcggc cgccctggcc tggaccgcgg cggcctcggc cctgatcctg
    18661 ggtcccgcgg tccgtgccgc gaagggggag tgagatggaa agccgagaac ccgccggcac
    18721 cgaccaggct cgaacggagc ccatcgtcgg cgtatcgcgc aacctggccc gcctggccgc
    18781 ggcggacatc cggatgctgg cccgggatcc cctgctggcc ttcatcccgt tcatcccctt
    18841 cctggccgcc ttcgtcctcc gtttcgcggt gcccctcctc tcgggcttcc tggagcgggc
    18901 cctgggcttc cgcctcctgg attgggcgga cctcgtccgg gccgtggtcc tcctcttccc
    18961 cggcatgttc tacggcatgg ccgcgggctt cctgcttctg gacgaccggg acgacggggt
    19021 ctccgcgtac tggggggtga cccccgcggg ccgcgccgga tacctgggag cccggctggg
    19081 gctcttttcc gccgcggcct tcctggcggg cgtcctggcc gcccgggccc tcgggctggg
    19141 gaggccggac ctcggccggg agctcggggc ggcggccatc ggagcggccc aggcggcctt
    19201 cttcggcctc ttcctggcgg ctttcgcggc ggacaaggtg gagggcctat cggtcctcaa
    19261 ggccctgggg ggcctggacc tggctcccct ggcggtactc ctggccctgc ctcttcggat
    19321 tgcggcctgg cccttccccc agtactgggc cgcggagctc atgctggggc ggaactcgcg
    19381 gtccgggatc gccctggtcg cgggcctggg aaccagcgcc ctttggatcg ccgcgctgta
    19441 cgggaagtac cggaaaagga tagagtagcc cgggccgccg agcgttccgg gtccgatagg
    19501 ggcggcccgg gagcgggaag ggattgcgga tcgaacctcg aaagctcggc ggcctcgcgg
    19561 ccaagtccct gggtgggatc cttcccgccg cggcggtcct ggtcttccgt cccctcggcc
    19621 tagacccggg gcagaccgtc gtcgcggcgg ccctcgttct ggccgtgggc ttctggaccg
    19681 ccggagcggc ttcgaaagcc ctggtctcgg cgttcctcct ggtcgtcttc ctggccttcg
    19741 gccggaccgc tcccgcgtcg gtcttccgct ttcccctgtc cgagtccttc cccgtcatcg
    19801 tcctggcctt cctggtctcc caggggatcg tgaagagcgg cctggcccga aagctgctac
    19861 tccccgtcct ggaccgccgg gcgggaaccc ccgggcggct cctggcggcc atgatcctgt
    19921 cctcggccgc cttcgtcttc ctcatccccc agcccttttc ccgggtgatc atcctgtccg
    19981 ggatcttcgc ggagtgcctg gaggcccggg ggcttccggc ccgcctgaag gagcggctgt
    20041 tgttcgccct ccacctgttc tcggccttgg cgaacaacct gttcctccgg ggggacctga
    20101 tcctgaatcc cgcctttctg aacttctccg gcctgtccat gggcgagagc ggctggacgg
    20161 cccggctcct ggtcccgggg cttgtccaca tggccctggc cctggtcgtc ttcctgctca
    20221 tggcgcggag ggattggcgg gagtacgccc gcggcgcgga gacgtcgcgg accgagggaa
    20281 ccggcaggcc ggcctcggac attcctgcga cagccccggc ctcgtcccgg ctttcgtcgg
    20341 cggaccggcg gaacctggtc ctgatcggcc tggtcctgac ggcctgggcc ctggagggcc
    20401 tgcacgggat acggggggtc tggatcgccg ccgcgggcac gggggcgatg ttcgtcctgg
    20461 gtctcctgga gccgaaggac ctcaaggccg tcaatcccgg gctcctcctg ttcctgaccg
    20521 ccgcgtcctc gataggggcc gtgctcgagg ggagcgggac agcggccagg attttccccg
    20581 ccctggcggc cctccttccg cgggcctggt ccccgggact ggccctggga accgtcggcg
    20641 tctccatggc gggccacatg gtcctgggca gcaacatgac caccctttcc gtcctggtcc
    20701 cggccttcag ggccgtcggg gagggggcgg gagtcccgga ggccgtcgcc ttcctggtct
    20761 tcgtctccgt gacaacccag ttcctcctgc ccttccacca cgtggtcctg ctcctgggcg
    20821 ccgaggcggg acgctacggc cgggggacgg tgttccggtt cggcctggcc ctgaccctgg
    20881 tctccgccgc ggccgcggcc ttcctgtatc cggcctggtg gggtttgcgg ggtctgccct
    20941 aggggtcacg tttgccaaac gacttcatcc gtgccacaat ccggggcatg gacgcgatcc
    21001 ccatcaacac cgacacggga ccccaggtgg tcctggagct catctatcgc ctgaaggtta
    21061 aggacgtcat gaccccggac ccggtcaccg cggcccccgg ggacaccctc cggtccatcc
    21121 aggaagccat gcgggaccgg ggcttctccg gggtgcccgt cgtggaagcc gggcgcctgg
    21181 tcgggatcgt ctccatcggg gacatcctgt ccgccctgga cgggggctgg atcggggaga
    21241 gggccgaagg ccggatgacc cggtccgtga tcgtcctgga ggacgacatg cccctgacct
    21301 tcgccatcac ctaccaccag cgctaccggt acggccgctt cccggtcctg gacaaatccg
    21361 ggaagctcgt cgggatcctg acccccacgg acatcatccg gtccctcctg gtggagatga
    21421 accgggaggt ggagcgcctg gagtcccggc tcaaggagaa cgccgagcct cccggaggcg
    21481 agacggtccg cctggccttc tccacggtgc gctacgattt cgagaacgcc ggccgggctt
    21541 ccggggccat caagaaagcc ctcaaggacc ggggggtgga tcccgcgatc atccgccgcg
    21601 tggccgtcgc gagctacgag ctcgagctga accaggtcat ccactcggtg gggggcacca
    21661 tcaccttcac cctggaaccg ggccgcgccg aggtgcgcgc cgaggaccgg ggcccgggga
    21721 tcccggacct ggaccaggcg atgcgggaag ggtattccac ggcggacgag tggatccggt
    21781 ccctgggttt cggggcgggg atgggcctgc cgaacgcccg caggagcgcc gacgagttta
    21841 ccatcgagtc cgcccccggc cgcggtacct ccgtgcgagt ggggtttgcg ctcaagccgg
    21901 atcgagcgta ggataggcca tgttcgactc aagcgcctac aagcgacggc gggaagccct
    21961 gagacggtcc ctggcggacc ggggcctttc ctcgggcacg gtcctcatcc tgggcaaccg
    22021 cgagagcccg atgaactacg cggacaacgc ctatcccttc cggcaggatt cctccttcct
    22081 ctacttcgcg gggatcccgg agccggacct ggccctgacc ttggacctgg agacggggaa
    22141 atccgtcctg tacggggagg agctctccct ggacgacgtg atctggacgg gcccccggcc
    22201 ctccctccgg gagctcgcgg aggctgcggg cctggacggg gtccggcccc gggcctccct
    22261 ggccggcgcg gcgggttccg cgaaacccct ccacttcctc ccgccctacc gggcggagca
    22321 ccgggaggag ctctccgaga tcctggggat ccccccgaag gcggtggaaa ccaaggcgtc
    22381 cggggacctg atccgggcgg tcatcgccct tcgggagatc aaggagcccc gggaggtcga
    22441 gcggatggac gccgccgcgg acctctccgc ggacatgcac cgggccgtgc tggcccgggc
    22501 ccgtccgggc gtccgggaat ccgagcttgc cgcccgggcc gcggaagtcg ccctcgcggg
    22561 aggcgcgggc ctggccttcc cggtcatcgc caccacgagg ggttcggtcc tccataacca
    22621 cgaccattcc ggcaccctcg ccgaggggga cctcttcctc atggacatgg gcgccgagac
    22681 cgtggacggc tacgccgggg acctgaccac gaccttcccc gcgggcggcc gcttcgatcc
    22741 ccgccagcgg gagatctacg agatcctcct gtccgccttc cgggccgcga ccgactccat
    22801 cgcccccggg gttccctatc gggacgtcca cttcgcggcg gcccgggaga tcttcacggg
    22861 gatgaaggcc ctgggccttg cccgggggaa caccgaggag gccctggccg caggggccca
    22921 cgccctcttc ttcccccacg gggtgggaca ccagatcggc ctggacgtcc acgacctgga
    22981 gagcctaggg gaggtgaacg tggggtacga cggccagccc aagagccccc agttcggtct
    23041 ccgatccctt cgcctggcca aacccctgaa gcccggcatg acggtgaccg tggagcccgg
    23101 gatctacttc atcgcccccc tcatcgccaa gtggaaggcc gagaagctcc atcgcggttt
    23161 cctggagtac ccgaagatcg aggagtgggt gtccgtgggc gggatgcgga acgaggaaga
    23221 ctggctggtc acggatgccg gggcccggaa gctgggcggc cccttcgaca aggacgccgc
    23281 ggccctggaa gccgcggtcg gctcggaatg gaaggggaat cgatgaccgg cgttcccgaa
    23341 ctgacggtgg atccctccct ggagccccgg aagatccgtg tgggtctggt ctggggcctg
    23401 ggcctggcct cctgcccgcc cagcgccggg ccttccgcgt ccctggccgc cctgctcggc
    23461 caggtggccg cggcggggga gggcttcctg gccccggaac gcaaggccgc ggtccggaac
    23521 atgctccgct tcggggccta caagcccgcg ggcaggtcca agccctccag cgagtacctc
    23581 ctggccgccg ccctggaggg ctgtttcccg atcgtgaacg gtcccgtgga cgccaacaac
    23641 gaggtcagcc tgcgctgggg ctacccggcc agcgtcttcg acctcgggaa gaccggcccg
    23701 ctcctccagc tttcccgggg cggtccgggg gagagcttcg tcttcaatcc ctcgggtcag
    23761 gagatcgacc tggaggacct gatcgtgatc cgccgccggg tcggcgacgc ctgggaggcc
    23821 tgcggcaacc ccgtgaagga cgccatggcc accaaggtct tcgaggccgc ccgggacgtg
    23881 gcgggggtgg tctacgcccc cctcacggac cccaaggcgg acctggaggc ctgcgcggac
    23941 cgtttcgccc ggatcttgcg ggaggactgc ggggcccggg aggcggggtg gaagattgtg
    24001 tgaagtgaat gggtgaatag gtgaatggat gaatgggtga atgggtgaat gggtgaatgg
    24061 gtgaatggga gcggacggga atctggagac attccgccga cccgtccgcc gtagccgttc
    24121 tgaacacctc ttcactattc actattcacg attcacggta ctattgcccc atggacgaac
    24181 cccacggcgg ctcgggcccc ctggtcggca tcatcatggg cagcgactcg gacctcccgg
    24241 tcatggccga ggcttcccgg atcctggagg agctcggcgt cccccatgag acgaccatcg
    24301 tctccgccca ccggaccccg gaccggatgt accggtacgc gaaggaagcc cgggagcggg
    24361 gcctggaggt cctggtggcg ggcgccgggg gggccgccca cctcccgggc atgtgcgcca
    24421 gcctgacgga ccttccggtc atcggggtgc ccgtccgctc caaggccttc aacggcctgg
    24481 acagcctcct gtccatcgtg cagatgcccc cgggcatccc cgtcgccacc gtggccgtgg
    24541 acgggggccg gaacgccggt ctcctggcgg cccgcatcct ggcgagccgc cgcccggacc
    24601 tggcggagcg cctccgggcc tggtcggccg cccgtgccgc cgaggttctg gacaaggcga
    24661 gtcgcctgga ggaggagggt ccggaagccc tcctctcccg gatctaggga tgcccgtgga
    24721 gatcatcgcg acctccgacg ccgacctgcg gcgggccgcc gaaatcctcg cctcgggggg
    24781 cctcgtggcc ttccccacgg agacggtcta cggcctcggg ggggacgcct ggaacacccg
    24841 cgccctggcg aggatcttcg aggccaagcg ccgccccgcc ttcgaccccc tcatcgtgca
    24901 tatcgccgcc cttgccgccc tggacgatgt cgcggacctg ggtgccctgg tcccggaagc
    24961 ccgtagagcc gcctgcgatc tcatggagga gttctggccc ggccccctca ccctgatcct
    25021 gcccaagcgc ccccgggtcc ccgacctggc caccagcggg cttcccaccg tggcggtccg
    25081 cctcccgaac cacccagtgg cccgggccct catagagctg tccaccgggg ccgtggccgc
    25141 gcctagcgcc aaccccttcg ggtacctgag cccgacccgg gcggagcacg tggccgccca
    25201 gctgggggac cgggtggacc tgatcgtgga cggggggcgc tgtcccgtgg gggtggagtc
    25261 cacggtggtg gatgtcaccg gcccggcccc ccgggtcctg cgccccggtg ggctgtccct
    25321 ggagcgcctg aggaccgtgg cgccggggat cgaggtcctg gaccggacgg atcccgcgcc
    25381 caaggccccg ggccagctgg ccaaccacta cgccccccgc cgggccctgc ggctggcgcc
    25441 cgcgggaggg ctttccgcgg cccggccgaa gggccgggct gccgccctgg tcttcgacgc
    25501 ggaaagccgc aggcggctgg aggaatccgc cgcccggttc gcggaaatcc gggtcctctc
    25561 ggaggcgggg gatcccgtgg aggccgcggc caacctcttc gacctcctcc acgacctgga
    25621 ctcgggggac tgcgaggagt tatgggcgga gcgggtcccg gaggcgggct tgggcgccgc
    25681 ggtcaacgac cggctctaca aggccagcaa aaaaacttga tcccgcacgc ttatttcgtc
    25741 gtataatcaa agcgctcggt tcctccgata atgagtatgg aggagagcga tgcagacgat
    25801 cgattccgcg tacagcctgc ccctcgccgt gatgctgtcc cggtacagcg cgcccggccg
    25861 cgtctccatc cccgtgccca gcagccaggt catctacgcc cacttcgagc atgtctccgg
    25921 agtcgccgcc ggcgaaggcc gggcgtcctc ggtggaccgc ctcaagatcc tgaacaccct
    25981 gatcgaccgg ctttccagcc tcaaatccga tccgctgacg gcccgggaaa gctccggtct
    26041 gatcagctcc gaccgggtgg acgccctcct ggagcagtac ggccgggagg cccacgccct
    26101 ggccgcccgg agcggcccct acgccccgag cctcggcctg cccccgggct ccctgatctc
    26161 cctggccgcg taagggccgg ccgcctaacg gtctttcaac atccccgtca gtgccccctc
    26221 ggcgtcgagt ccgtagtgga ggactccgta gaccacggag gcccagagga cgttccggcc
    26281 gtactggcgg gtctcctcga tggacaggga ttccagcaga aggtcgtccg ggaagcccgg
    26341 ggccgcgcgc tcccactggc gcatccgcga gagccccgcg ttgtaggcga agaccgaggg
    26401 caggacccgg ccgttcaggg cccggagcag acccgcgaag tgggcgctgc ccaggcggag
    26461 gttgtccgcc ggattctcca ggtcgtagga gcccatgcgg agccgtcgcg cggtctcctc
    26521 cgccgtggcc ggcatgagct gggccaggcc cacggccccg gcccgggaga cgaccgcggg
    26581 gcggaagaag ctctcggatc gaacgagccc gtacaggata tgctcctcca tccccgaggc
    26641 ggccgtggaa gccgagacct ccgcgggcca gggccggggc cagagaagtt cccagtcctc
    26701 ccgggaggcc tcgtagccgg gggtctcgaa caggcggttc accacgagca tggaggccgc
    26761 cgggtatccc cggtcccgga ggagggccgc aagtcggcgc agggtctccg ggtccggctg
    26821 gtcgggggat ccttccaggg cccggacttc ctgggggacc aggtccgcca gcccgtagag
    26881 ggcgaacccg gccaggtagt cctcggggtc cggtcccgag cgccgaaggt cccgggcgga
    26941 ccgggattcc ggcttccggg gctcgacggg gcgggacggg gggagctcga ccagggggag
    27001 tcccagccgg tgggcggaca ggaggcggta gtagggttcc gggctccggg cccgcacctc
    27061 ctcgaaggcc tgccgtgcgt actccccggg gtccggggcc gtaccgtccg aggcccgggg
    27121 cggggcgatc ccggcttcgg cggcgcggcc ggccaggtag acgaggcgga atgccgcccg
    27181 gggactcatc ctgggcgcca cctcggtgcg cagggtgtac agggtcgcgc cgtccttggc
    27241 caggagagcc tcccgggcca ggcgttccag gatgtccgcg aaggacgcgg ggttgtgcca
    27301 ggagggggcg gtccgggcga gccaggcggc ggcttcgcgg cggccgccgg gaaggaggcc
    27361 gtccgtccgg taccaggccg cggcgtcccg gtcctccccg gatacggcca gctcccaggc
    27421 ccgtccgaac tcggcggccg cctcggcctt gcgttccagg cgggccagca tccgggcccg
    27481 gtagaatacg gcgatgaaga gggcctcccg gggatcggcc tcgccgcccg cttgagccgg
    27541 ggttccggcc gacccctgcg cggcggcggt ttccagggcc gtcatcgcgg cgatcccctc
    27601 cgccggggcg ggggcgtaga gataggcctt gcccgcgtcc gaaagatagg cccggggcag
    27661 gtcgggcgac aggagggcct gtgccggcgc cagggcccgg taggcggccc cgtagtcccg
    27721 gcgcgcgatc tggagccgca tacggaagag ggccagctcc ccttcggaga aggccgaggg
    27781 atccgctccg ccggtcgccg cgtccggggc cgcggcttgg gcggccgccg gggcttccgc
    27841 ggcgcccagg gcggcctcca gatcctccgt ccggggggag gccagggcga agagctcccg
    27901 gatccgggcg gcgcgttccc cgggatcctc ggcggcccgc atccggaggc ccgcgagcag
    27961 ggcctcgtcc cgggcggccc ggtcgggcca ggcggcggcc agggcgtcca gctccgcccg
    28021 ggtttcggga aggcggccca gggaggccag ggcctcgacc cggcgccggc gggggaggta
    28081 gtcgtcgatc cccgcttggg cctggcgctc ggagaaggcc aggagccccg gccagtcctt
    28141 cttccggtag agggcctcgg gaaggagctc ccaggccctc ctgcggaaga gccccgcctc
    28201 cttccgggaa gccaggtcca ggaggacggc ttcccgctcg gggtctcccc cgaggcccgc
    28261 cgcccgggcc gcgtagtaca gggccccggg tcccagacgc gccagctcgg ccaggcgctc
    28321 gggaggggcg gacaggacga gggaacggtc cccggccagg agccggtccc ggagctcgtg
    28381 ggcccggacg cccaggacca tgggatcctc gcaggatccg gtcagggccg cggccgcgat
    28441 gaggtagaat aaaaaggctc gcggcgggtg ccgcgggcct ttcacggggc tcgaaacgct
    28501 cgatcgactc aaacgggctc ctcgtcctcc ggcggcctgc cctcccttac cgggggggga
    28561 tccagatcgt gcggccggag atgatcaggt tcgggttgcg gatcttgttg gcccgggcga
    28621 tgcgcggata cagccaggga tcccggtaga agacgtagga gaggtcccag agggtgtccc
    28681 cccatcggat cttgtagttc acgccgggtt tcatgcccgg ccgcgcggcc acctcggggc
    28741 gcacgtaggc ggccatgccc gcgggcaggg ccttgggggg ctccgccggg gcgggggcgg
    28801 ccggctgggc cgcgggggcc ggcgcgggct ggaccgcggg ggccggtgcc ggttgcggtg
    28861 ccggctgggc cgcgggagcg gcgggctgtg aggccgcggc cggcttcggg gccgtgacgc
    28921 accggtagat gatcagtccg aggcctaggg ccagggccag ggccgccagg acgatcagga
    28981 tgatccagag cgcccgaccg gatccccgct ccttcttggg cttcggttcg ggtgcgggct
    29041 tcggagcggg ttcctcgtcc tcgaagggat tggcgaccgt cccgggccct ccgggggact
    29101 cctcgaagga gggctcctcg aaggaagcat cctcggggaa gggctccccg aaggacgcct
    29161 cctcggcttc ctcctccagg gtgaaggatt cctcggactg tccgatatag gattccccgc
    29221 cttccggggc gggctccggg ccctcctcgg acaggaggtc ctcggccggg ccggcgtcct
    29281 cgagatcggg cgctttcggg acggcctccg gggattccgg accggccatc tccgggacct
    29341 cgaactccgc ggactccagg tccggcagcg tgtccgtcgt ggcggcctcc cgggactcct
    29401 cctcgaactc gaagtccggg atctcgtact tctcctcctc cgagagggcc tgcagggaga
    29461 cccggaggct ctggctctcg ccggaggctt cctcccgggc ggaggcgagc agttcccccc
    29521 cctcgtccag ggagagctcc aggcggatgt ccggctcgcc cttggggcgg ggagggatgt
    29581 tctcgatgac caggctgccg atgtacgccg ccgacgccgc ggtctcgccg gagccgcggt
    29641 acatgtcgat ctgcacgctc ggctggtcgt ccttcacggt ggtcacgacc aggcgcttgc
    29701 gggcgggctt cccgtcctcc aggatggggt agaattggcc gttggccagc tttataccga
    29761 tgttcctatc cgacatggtg ccttccatcg gcccgcagcg tgcgcgtgcc ggcgggggcc
    29821 ggccgacgcg cgagccattc gttaattatc taagtatatc ggctgaaatt agggaaagca
    29881 aatcgggaaa cggtggcctg tggtcgggtg aatgtggact caggactcgg gacgcggcct
    29941 aggccggggg ctcgggtttc cagcacgcga tgaggagggc gaggagcccg caggaggccg
    30001 cggacagcca gacccaacgg gggacgcctc cgaccaggac cggcgggacc ccgtactccc
    30061 aggcgacggt ccccagggcg gccgtcgcgg ccagcgcggc gagccatccg gggcggaacc
    30121 gggggaggat gcccgtacgg ggggcgggcg gctccctccg gccgtccgcc cggccgaagg
    30181 cggccaggac cagggcctgg gcggccaggt tcacggccgc gggcggaacc gggccgaagg
    30241 tcaggcgtcc ggccgactcc aggacgacca gggcaaggcc gagggccagg gcggcggccg
    30301 ccccccgggc gttcgcgcgg cgcccgtaca cccccgccca caccgggggc gcgaggacgg
    30361 cgtaggccgg gaaagcgatc ccggtcaggg catcgagtat gaggtccggc ggggagaggg
    30421 acgcgatcca ggccagcagg gcgagggccg cgacgagggc ggcgtcggtc cggcgggatc
    30481 gggcccccga gggcgcgaag tcctggacga tcatcgagga cagggacagt agctgggagt
    30541 ccagggtcga catgagggcg gagaccgcgg ccagggcgaa gagggcgccc gccaggggct
    30601 ccgcgcggat cgccgccagc cgggggaaga cctgctcgga ttccgccggc ggcaaccccg
    30661 gcaggagggc cgccccggcg acgccgacgc cgacggtcag gaagaacagg atcccgcaga
    30721 tgagggggta gaggatcgcg gcccgatgga ggctctcgtc gtcccgggcc gcgtagaacc
    30781 tctggaacat ctggggaaag aggggatccg ccaggaacca caacagccac agcccgagca
    30841 ggcgatccag tccgaccccg gcgccggcgc cgtcccggga gaggtgggcc ggcgaggacg
    30901 cgaggagccg ggccgccgcg gacccgtccg ccatcccgag gacggccgcg aacgccgcgg
    30961 ccgctcccgc ggccaggacc gccagctgga gggcgtccgt ggccgcgacc gcccggaatc
    31021 ccccgcggca ggtatacaag gccgtcacgg cgacgagcag gagggaggcc gccggatagg
    31081 ggatccccgt caggatcgag atcatccggc ctcccgccac ggcctgggcc gccaggtagg
    31141 gaacggtgac cgcggccagg cagagggaga acgctttggc cagggcgggg ctcccgtagc
    31201 ggtcccgcag gaagtctgcc ggggtcaggt atcccctccg cgcggagagc cggcgcaggg
    31261 gaatgccgag gaacaggaag gccgcggcca tgagccccgt gccgaacccg atggcgggat
    31321 aatacgccca gccgatcctg taccccgcgc cggacaggcc gaacacggtg aaggcggaga
    31381 agttcgtggc cgccagggtg gcgaacagga ggagggggcc ggagcgcgca ccgcccagca
    31441 cgtagtcccg gcgggcgccg gggtcggtgc cgcggcaggt cccgcgggcg gcggccgagc
    31501 cgacaaggcc caccgcgagg aggtacaggg cgacgagcag gaggggtagg ggcatgccga
    31561 cgctccgcgg ccgcgctccg ggagggcgcg gcgattcggg gatccgcccg cggaggccgg
    31621 aggggccgtc cgggaggcgg ttcggagcct gggtggccac tcaaggcttc ggcatggctc
    31681 gaggaccgga ttctacccgg agccctccgg gaacgcaagg gccacgaggc tctccgcctt
    31741 tcggtcgtag gccgcgccgg ccagggagcc caggatccgg acctccccga aacccgcccg
    31801 ggccaggagg gccgccatct cggtgccgga atagagccgc tggacgaagg tccgctcgat
    31861 ccgctcggcc ccccggatca ggatccagcg gttccgcatc ccctcccagg ccccgaccgc
    31921 ggagtattcg gtcagcacgg tccagccgtt ccgctcgaac cactcgccct cggtgaagtc
    31981 ccggaccgcg gtctccttgc ccagcatctc caggacgaag cggcccccgg gcttcaggga
    32041 ctcccggata ttgcggagca tccccaggtc ctgggcgggg tccgcgaagt agccgaagga
    32101 gttgaagagg ttcacggcga ggtcgaaggc cccgggccgc cggaactccc gcaggtccgc
    32161 cctaaggaac tcgatgtcca ggccctcgtc ccgggcgctc tcccgggcgg cctccaggta
    32221 ggagtccatg aggtccaccc cggtgacccg gtagccccgg cccgcgaact ccaggctgtg
    32281 ccgcccgggg ccgcagcagg cgtccaggac cgcgcccggc ggggaggcgc ccgtcagacg
    32341 ctcgatcgcg tccacgaccc cggggacctc ggcccagcgg ttctcgtcga acatgagggg
    32401 ggcgaagacg tcccagaact cctcgtcctc aaaccactcc gtcccccggt ccgccacgtt
    32461 agaagccccc gtggtccagg acccgctcca ggacgtagct cagaaggtcc atgtccaggg
    32521 tctccccctc ggcggagaag agttcccgga tctcctccca gtcctcccgc tccaggggcg
    32581 gatcgtcctg gtagtccatc agggccagga ggcaaacggc gtccagccag gcccgggcgc
    32641 gggcggcgcc accgggaccc cgggtcaggg cggaccagcg ctccgcgatg ccggccagga
    32701 ggtccccggg gatgccccgg agggagcggc ccaggagggc cttgagggac tcgaagcgcc
    32761 gcgcctcggg gtcccgggcg gtgcggcccg cccagtcgtc caggtcggaa aggagccggt
    32821 cggagggtct catggggaaa agaataacgt caaaccccgg acctgcatag ttcccggagg
    32881 cggcccggcc gtttaccggg ctccgtacac ggccgccagg atcgcggcgg catcgtaggc
    32941 cgcgtgggcc caggccaggg cgtggaggct ccgggtgcgg ccccaggccg cggtcaggag
    33001 gacccccgag gcggccgccc cgagcacccc cgccgcgccc tggtaccggt gggccgccga
    33061 gaacagggcg atggagaccg ccgcggccgc ccagggaggg gcgccgtccg cttccaacgc
    33121 tcccgggagg tagacccgga acaggagctc ctcccggtag cccacggcca ggcaggaggc
    33181 ggccaggagg ggcaggaaga gggcggccgg gaggtccgat gccgccgccc tcccgggggg
    33241 cggggcgccg ggcaggaggg ccgcgccgga agcggcggcc cagaccgtca gggccaggag
    33301 gggcaggagg accagggccg ttgcggcgtc ccggggaagg ggccggcgaa gatggaactc
    33361 ccgggatcga ccccccgcgc cgatgatgta aaggagcagg agggtctggg gtaccaggac
    33421 cgccaagttc cccgcgaggc gggatcccga ggccagggtc tcgaccgcgg cggcgtccgg
    33481 cgcggccgcc agggagggca ggacgaagac ggccgccacc aggacggcct ccgcccggat
    33541 ccaggggtgc tctcggcccg gggtctccat gcgcggaccc tccttcggat tttccgtttc
    33601 cggggcgata caatcgaatc gcctccgcat tttgattatg tgtcgttttt tatagtatag
    33661 ttgactccgg gcggcaaggc gcggccgccc gaaggcccga gccggggacc gggttcccgg
    33721 gcgatcggcc ttggagcgcc gggagtgccc atgatcgcag tgctcgccgt cgtggttttc
    33781 atcctcttcc tcctgaccat cacgctgggc tcccggggcg acaagcggta ctcctggttc
    33841 gagtactacg ccaagggccg ggagtccggc ttcaccttcc gccaggccaa ggacctgaag
    33901 gacctggccc aggtcgccgg gctgggggat cccacggaag tcttctggag cctcaaagcc
    33961 ctggaccggg tcatcgcggc catcacccga tcccggaacg cctccccgaa ggggctggac
    34021 cgggagaccg agcagcttct ggaacggatg tacgagtacc gccaccgcat ggaattcgac
    34081 cagccgaagt acaagatggg gatcaagagc tcccaccagc tggcggccaa ccagcgcatc
    34141 cgcatcctgg tgcacggcgt cggggtcttc aactcgaccc tgatcgacgt ccatgcccgt
    34201 tacctggtct gtacctaccc catcggggtg aagcttcccg cgggcttctc ctggaagggg
    34261 cggcgggttt ccgtgtactt ctggaggcac gaggacgcgg gctacgtatt cgacacctac
    34321 gtgatcgacg acctccgcat ccggagcgtg ccggtcctcc acctggccca ttcggaggcc
    34381 ctcttccgga cccagaagcg caagtccgtc cgcgcccgat cccggatctc cgcctacatg
    34441 tacctgctga aacgggtgga gggagcctac gagaaacccg agcgggaccc gggcctcaag
    34501 tgcgtggtcc aggacctctc ggaggacggc ttcgccgtga tgatcggggg caaggcccgg
    34561 gacggtatct tcgtgaagat ccagttcttc ctgggggagg accaggtggt gatgagcggg
    34621 accgtgaagg gattcgatta ctggcccgag aacaaccgtt ccctggtgca cgtggaggcg
    34681 gtgcgcccga gcccgcgcat ccggaacctc atccggtcct acgtgtacgc ctcccgggct
    34741 tccgatccgg acaagggcgg cggcgaggtc ttcgcccccg tgcacgcatc cgcggggagg
    34801 aacggatgat ccgtaacgaa tcgggcccgg aaaccctttt ctatacgggg gcgcccggcg
    34861 ccccgtccgc ccggtcggga atccgccgcg tctgcctccg cggcgcccgt ctccgggggg
    34921 gaaggagtgc tgacgtcatg gtgcgaattc tcgccgcggt cctgatcctg ctcgtctccg
    34981 ggggctcggc cctcgcccag gacaccaagg ccatcatcga gaccttccag aggaacttcg
    35041 tgcgctccag cctcggaacc aagctggagc tcctccggga ggccgcgggc tacgagggcg
    35101 tggacatggg gcccttgtac gagacggcgg tgaaattcgt cctggggaac gcctccctcc
    35161 tggaatccga cctggtcatg cgggacatcg ccatcgtctc cgccgagatg atccggaaat
    35221 acaagtacgt gcccgccgcg gaatccctgt gggacctctt ccagaccttc cgggaaaaca
    35281 gcgtccgggt tcccatcctc ctggccttgg gggagacggc ccaggacaac ccgaggctca
    35341 tcgaacggct caacgcctac ctgggagccc agacgaccct gttccggtcc ggctcccagc
    35401 cggactaccc gaccctggag gcgtgtatcg aggccctcgg ccgcctgggg gacggttcgt
    35461 ccttcccggt cctgttctcc gccttcgtgg cggggtaccg ggtcgacgtg acccagaagg
    35521 cctccgcggc cctggcgggg ctcaaggggg attaccacgg ctacctggtc caggtcatag
    35581 agcgcaacac ggccctggag aagcgggccg ccctggacgc ggggctttcc aacccgggct
    35641 tcacggggga ccagcgggcg gccctggccg aggcggccct gagcgccggg atcggctact
    35701 ccagcgggaa cgccgtggag accgaggccg tgcgggagat gcggtacaag gccgtgcggg
    35761 agctgaccga gcggaaatgg cagagggcgt cccccgccgc gatccggcac ttctacgacg
    35821 tcctgtccct gttcaaccgg agccaagcga ccaagggctc cctgctggag gccgtggcct
    35881 gcctgggcgc catgggtacc ccggaagccg cccagaccct gtccctctac ctccagctga
    35941 tcaacaccga gaccgagcag ggcaaggtct tcgacgagca gaccgtgatg gcggtgatcc
    36001 gcaacctggg catcctgggg gacaaggtgg ccttcgatta ccttctgtac atcggctacc
    36061 tcaagtaccc ggactccatc aagaaggccg ccaaggacgc cctgcagaac ctgaaatggt
    36121 gacgatgtgt ctggaaggga tcctatttct ggaataaacc acggagctca cggagagcac
    36181 ggagaatcag gggcttggga tcaaactttt gacaggattg acgggatcaa gttcggatga
    36241 acaggattcg gtacgggata ttaaagatcc ttcagattca acccgatccc gggtgtcgca
    36301 atttctcgaa aagcgtgagc ttttcgagaa attgcatagc ggggttctga caagctcgga
    36361 gcttgtcaga accccgctag cccccgctga ccaagtggtg caggtccacc accgagccgt
    36421 cccggaccac gacggaatcc cagtctttgc gctccaccag ggtcccgtcc acccgggcga
    36481 tgatcagggg aaagctccag cccttgagat ccaggatctg gcggatggtc agggattccc
    36541 cgggaatatc ctccgggttg tggttcaata cgatcttcac gctcgccccc ggcccgacgg
    36601 ttcccccggg cctacgggta taagctacca aagccttccg gacgggtaaa gggcctgtaa
    36661 ggatgccggc cgcccggagt accggccggg tgtcacaatc gcttccgcgt tcctccgcga
    36721 ttctccgaac cggttcccca aattctcgaa tatgcatata ctgacgccat ggcagcggct
    36781 cccgagattc ccggaccccg gcggggctcc agcctccgca cggccatcct cctgcccctg
    36841 gcctccctcg tgctcctggg cttcgcggcc tcctggacgc tgtacgtcct ggcttcccgg
    36901 gcttccatgg gcggggtcgt ggagcgtctg gtcctggaga ccaccgccgt cctggatgcc
    36961 cgtctttccc gcatcctgga ggcgccccgg cacgtcggca tcacggtcgc cgagttcgcc
    37021 cggcaggtgg gggattccga gtcgagcctg gacctgttcc agcgggtctt ccgggcccag
    37081 ctggggaagg ttccggaact caacatcgcg gccatcgggt tccgggacgg ggagtacgcg
    37141 gaggcccagc gcctggggga cggccggatc ctggtgggcc gtgccggccg ctccacgggc
    37201 ggggccctgg agagctggac ctgtgacgag gacggtaacc ccggcgagct gtacgcgacc
    37261 cgtccgggct acgacccccg gacccggccc tggtacctgc gaagcgccga aaccaggata
    37321 cccggctggt ccccggtgta ctcctattac tccaaccagg atctcgccgt cgccgcgggg
    37381 tttcccgtcc tcgcgggggg cgaggtcctg gccgtggtat ccgtgacgac gaccctcaag
    37441 ggcatccacg atttcctcaa caccatgccc caggcccgac ggggcgtcct ggccatcttg
    37501 gaccgcgagg gccggacgat cgccgtcacc gggaagtacc cggtcagcct ggacggggac
    37561 ggcatgctcc ccgcggcgga ggaatccggc aactccctgc tcgccaaggc ctctccgcac
    37621 ctccccctta aggtcaaacc cgagtcgtcc agcctgaggt tccggttgga gggggttcgg
    37681 tacatcgccg ggatttcgga gcttcggaac gacgaatggg acctgggctg gagggtggtc
    37741 gccatccttc cggagtccga gttcacggag cggatgaccg aatcggacgt gcggggaggc
    37801 ttcgtgttcc tgctcctcct gtccctggcc ctcctcgtgg cgtggctgac cgtggacgga
    37861 ctgacggagc ccctgagcca gctggaggcg gcggtccggg acctggcctt cgcccggttc
    37921 tccgaggatt ccggcgggac ggtccgggcc ctggcccgcc gcagggacga gatcggccga
    37981 ctggccgcct ccttcgacaa catgcgttcc agcctgttga tcagcttcga gaccctgcgc
    38041 tccaacctgg aggaaaagga ggtcctcctc aaggaggtgc accaccgggt caagaacaac
    38101 ctccaggtaa tctccagcat gctgtccatc caggccgggg agatcgagga tcccaaggcc
    38161 ctggaggctt tcgcggcctg ccaggaacgc atcctggcca tggccttcgt gcacgagggg
    38221 gtctaccagt ccggcctcta caccgaggtg gagatggagg aatacctggg ccggatctgt
    38281 tcctccctcc ggtggtcccg ggccgcggac gctccccgga tcgacatccg ggtggacgcc
    38341 cgggagatcc gcctgccttt gtcccaggcc atcccctgcg gcttgatcgt gaacgaactg
    38401 accgtcaacg ccctcaagca cgccttcccc ggggagcgcc cgggcgctgt ctcggtcgtc
    38461 ctggcccggg aggatccggg gtaccgcctg atcgtggagg acgacggcgt cggggaagtc
    38521 ccttcgggga cctccgtacc ggggatcggg cgcaccctgg tggagtccct ggtctcccag
    38581 ctccggggga cggtcacggc cgccgcccgg cccgacggct ccggcctccg ggtggatatc
    38641 cgcttcggca ccctctagcc cgtcgggaga aaggcttcgc ttgccgattt cgggaattcc
    38701 gggctatact gtccgggctt catacgggaa gtaatcagct aggacggctg atacatcagc
    38761 aacatcttcg aatacctcga tccagctcaa agaaggaggt ccgcgagcca tgatcaagta
    38821 tcttcacacg gacgactcga agtgcgtcgg gtgcatgacc tgtacggcgg tctgctccca
    38881 gatgtacttc aaggaggaca acccggccaa gtcctgcatc caggtcttcg gcatgggcaa
    38941 gggggccttc cacctggtcg cctgcgacca ggaatgccgg aagtgcgtaa tggagtgccc
    39001 gacccaggcc atcaccgtgt ccaagaccgg ggtcgtgatg atcaacaaga gcctctgcgt
    39061 gggctgcctg gcctgcgtgg ccgtctgccc gatcgaggcc atgcgctact atccgggcca
    39121 caagaacccc ttcaagtgca tagcctgcgg ggcctgcgcc aagaagtgcc ccaaggaggc
    39181 cctgtccgtc cgggagaagg aggagagccc ggccgcccgc aggcccttcg aggtcgccgc
    39241 cgccgtgtcc cagggaggga aggaatgaaa ttcgacgcca aatccttcaa gctcctttcc
    39301 gagtggacct acgagaagaa acccctggcc aggggctaca acatgcgcac cctccacgtg
    39361 gacgtgggga cccggaaggt ttccgagaag cccgtgtccg aggacatgaa gaagcgcttc
    39421 atcggcggca agggcttcgg gctgcgcctg ctctgggacg ccaccaagcc ctccaccacg
    39481 tgggattccc cggaaaacga gatcgtcatc gccatgggcc ctgtgtgcgg caacacgaac
    39541 taccccggct ccggaaagag tcttgtggtg tccctgtccc ccatgaccgg ggtgcccatc
    39601 gactccaacg tgggcgggta cttcggcccc tacctcaagt tcgggggctg ggacgccctg
    39661 gagctccagg gcaaggcgga ccgggagacc atcgtcttcc tggacggcaa cgaggggctc
    39721 gtgcggatct tcgaggcccc cgccgacctg ccccaggaca cccacctcct ggcggaggcc
    39781 ctgacccacg ccttcgcgaa ggacgagaag gacctccagt ccgtgtccgt ggtctccgcg
    39841 ggccgggggg cggaccattc ccccatgggc ctcctgaact tcagcctcta cgacaagcgc
    39901 cgggggggct gccgggtcaa gcaggccggc cggggcggca tcggcttggt cctgcggaac
    39961 aagaagatcc gcgccgtggt ggcccggtac aagggggtcg agcaggactc caacggggcg
    40021 gcggatcccg cggcggtcca gcggatcggc ctcaagctcc accgggagat cgtggtcaac
    40081 gacgacaagc agtgcaagat gcgcaagcag ggcacggccc acctcatgg
//
LOCUS       DAOTEI010000007        39990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_664_length_39990_cov_5.930512, whole genome shotgun sequence.
ACCESSION   DAOTEI010000007 DAOTEI010000000
VERSION     DAOTEI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 39990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39990
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_664_length_39990_cov_5.930512"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="P5313_01225"
     CDS             complement(<1..597)
                     /locus_tag="P5313_01225"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HRY79013.1"
                     /translation="MDKSRKPGSRIAALAFPLILAAAVGGGILLWQPLTGIFRDPQRA
                     RAWVESLGAAAPLAYVGLQFLQVVVFIIPGEVVQIGGGYVFGFWGATILSSLGILAGS
                     VFNYYVGRLAGRPFVERLVKPEVLARLEKAAASGREAAGFFLLYVIPSIPKDALSYVV
                     GMSRISLPLFILISGIGRLPGILGSAFIGNSAYRGEYGT"
     gene            941..2215
                     /locus_tag="P5313_01230"
     CDS             941..2215
                     /locus_tag="P5313_01230"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRY79014.1"
                     /translation="MKIALAYSGGLDTTVIVPWLKETYDCEVIAVCGDVGQDCDWADL
                     EKRALSSGASKLIVKDLKREFVEDYLWPLVKAGAAYEGRYLLGTAAARPLLASSLTET
                     ALAEGADAVAHGCTGKGNDQVRFELGIRAAAPHMDIIAPWRIWNLSSREDEIAYLEAR
                     GIPVPVKVEDSYSRDDNLWHVSHEGLDLEDPALEPKWEGMLKLCCTPEKAPNVPEYVE
                     LGFEGGVPVALNGVRMGGVELVSALNVAAGRHGIGVLDMVENRVVGMKSRGVYETPAG
                     TVILEAHAKLEQLCLDRKTLSFKLQAAQRFAELLYEGEWTSPLMEALRAFVDSTQKVV
                     TGTVKLKLYKGAILGAGVASAYSLYDKSLASFATGPLYDHRDATGFVNLTALPTKARS
                     LVNSRLAARKAAAGGGDPTFRYGTGRVAASAP"
     gene            2241..3635
                     /gene="argH"
                     /locus_tag="P5313_01235"
     CDS             2241..3635
                     /gene="argH"
                     /locus_tag="P5313_01235"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRY79015.1"
                     /translation="MAKLWEVGSSGLDPRVDAFLSSVAVDGRLLAEDLEGSIAHARML
                     GKQGILPAASAEALVSELEALRSEAASGSLVPDPKAEDVHSFVEGELTRRLGDAGRAV
                     HAGRSRNDQVAVDLRLFLKKAFARARSGSLSAISAILDLAEGHTATLMPGYTHLQRAQ
                     PVTFAFHLLAWCAALERDAGRFADGLARADECPLGSGALAGSGLPLDREATAKDLGFC
                     RPSRNAMDATADRDACVEYAAAASTLLMHLSRFCEEVVLWSSEEFGFVKVGPACSTGS
                     SIMPQKRNPDTAEVIRGKAGRVFGGLVSLLVLQKGLPLAYGRDLQEDKEALFDVVDTV
                     DGCLESFAILVKSLEPNLERMRRAAETGFLEATDIAELLVLKGIPFRSAYGAAKQAVL
                     TCLSLGLRPADLSNSDLAGISPLFARAGIRAEELAAYLDLDACVARRNGTGGPAPERT
                     RDEIARLRAFAAGW"
     gene            3643..4245
                     /locus_tag="P5313_01240"
     CDS             3643..4245
                     /locus_tag="P5313_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRY79016.1"
                     /translation="MFDCIIPAAGASSRMGAWKPGLAFRDGTLLSAAAARALEAGCRV
                     IVVAGRAADSVPGALGSRGDRVEIVLNPAWEEGMMGSLQAGMARVSSPFFFVLPADMP
                     FVPARAFRLLAREAEVRAAAGLPESPVFPAFRGEPGHPVLIPSSLIREALRLPRDARF
                     KDFLEARGPVYVEVGDPGIRTDLDTRSEYEGALRLVSRSS"
     gene            4313..5326
                     /locus_tag="P5313_01245"
     CDS             4313..5326
                     /locus_tag="P5313_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79017.1"
                     /translation="MNLSRSRSVPVAALVLIVAASAHSQVPPARIIMGGRAAVLVADA
                     VYAFPSARSRVITVGGGDQGLGTFLQELDPSYPSKPVLDRNAAAEVYAAYRPDLAIFK
                     SALKRGVGGALESLGIRVHYLDLETPEDYYRDLEGLGRILGEPSRAAELVAYYKGVVE
                     TAARISAGASRKPRVLVVQAVGDAWEVPPASWMQTRIVEMAGGVPVWIGANPGEGWAR
                     VGLEQVAAWNPDVLLVIDYRRGVDEAVARMRADPRFSLLAAGKSGMIRGFPQDFFSWD
                     QPDTRWALGLLWTVKTLHPDRSGTLSLEAEARRFYRVLYGFDDAAFDRAVRPRLTGDH
                     GIR"
     gene            5313..6329
                     /locus_tag="P5313_01250"
     CDS             5313..6329
                     /locus_tag="P5313_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY79018.1"
                     /translation="MASGSADAPRPAGRVRAAGLWAAAFLLFALALLAGRYPNPGFLD
                     LRLLASDDLARTILLSVRLPRVLAAALLGAVLAASGNAFQMVFSNPLVEPGFLGVSQG
                     AAFGAALALVLGFRHDLAVSGSAFVFALAGLGASAALARRFRFGGWVLRLVLAGIAVS
                     AFFSSALAWVKYAADPLRELPDIAYWTLGGLSGIGWKRLAVVAPIALASLAALRAAGW
                     RLSILSLEDSASFSLGARPGRERALVLAAAVAGVASMTAVSGIVSWAGLIVPHAARLL
                     VGADGRRSMPASIALGAAFLMACDALSRSLLPGELPLGVTTAFLGTAGFTALLLGRVV
                     TVVR"
     gene            6329..7138
                     /locus_tag="P5313_01255"
     CDS             6329..7138
                     /locus_tag="P5313_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79019.1"
                     /translation="MLEYRQVCLRYPEGDRDAVAGFSLSVPEGSVRALLGPNGSGKST
                     LLLAALGWMRPRAGEVLLAGRPVSAWSPGERARKAAHLSQIERVSFTYEVLEFVLMGR
                     APHLAPLAEPDEGDEERARAALDALGIGDFARRHVTALSGGELQLVRLARCLVQEAPL
                     VLLDEPTAALDPANALRVADALASLAREGRTVVFSTHDAALAAYASDSVVLIREGRLL
                     ASGPSAEILTPELLSRTFAVPFGVSGAPSPFVTGSSIRAAGGGRGSGSWPR"
     gene            complement(7077..8300)
                     /locus_tag="P5313_01260"
     CDS             complement(7077..8300)
                     /locus_tag="P5313_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79020.1"
                     /translation="MSFFAVSDQTLADLAARAPGPFFAYSRSAARERWDRLAAVLPPR
                     VRLAYAVKANPNPGLLATFARKGAAFDCASGGELDRAARALAKARPLAGTRTTAAGRG
                     GPGPGNLLFYGGPGKRAEEIRRALRLGARIHAESVEDLALVDRFSRKPAAVNLRVHPA
                     SGIEEGSRIIGGTGPSAFGVDEENLARVLHVAKRFTRVRIEGIHVFAASNERDADKLA
                     RTYDTVLGLSRKAAEAFGLDLRQIDLGGGLGLAYSPGEEELDVEALGRSLGSLLERHD
                     WFRGELVLEPGRWLAGPCGVYAARVVRVKESRGVRFVILEGGINHLLRPLLTGQPFPV
                     RAFRADGSSIEEEAGPAVLTGPLCTSLDRLGDVLLPGLDSGDFVAFGRTGAYGFTEAM
                     SRFLCHPLPREYWIP"
     gene            complement(8297..9427)
                     /locus_tag="P5313_01265"
     CDS             complement(8297..9427)
                     /locus_tag="P5313_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY79021.1"
                     /translation="MRPAARMEGLGLSVIRALNEGAPAGCVSLGLGEPSWELPREARE
                     ALAAWASSEGSCPYGPNAGTPELRAVFRDFLGATDAEFLVSAGSQGALFSLVHAWAGP
                     GDQVLLPDPGFLAYPTLARLAGAEPVPYPLAPDFSLDPGLFRAVLDSAPRTRLAIVNH
                     PANPTGAGASRGALDEVYAACRARGVVLVSDEVYRELYLDERPAGLADTGIRPGAVVL
                     GSLSKAFAAPGLRLGWAHGDPEVLAPARLVHNAMVSCLSRPAQAAAAALIASAGSVFP
                     RARSELAVRRETLRSAAREHLGWDPPAPAGGFYAWLALPPAGRADPLSFCLAVRDRGG
                     VIVVPGSAFGTRGASYARVSWAGSPVDITEGVRRLSAFWRDQ"
     gene            complement(9424..10149)
                     /locus_tag="P5313_01270"
     CDS             complement(9424..10149)
                     /locus_tag="P5313_01270"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011045222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRY79022.1"
                     /translation="MAALESGEVRAAFRGPDGTWTAVTWVKRGILAGFKAAPVISFPA
                     WPGGARDKASYPPREISEADGVRMVPGGSSVRRGARLGRGVVVMPPAFVNVGAWIGDG
                     TMVDSHALVGSCAQIGERVHLSAGAQIGGVLEPAGARPVVVEDGAFVGALAALLEGVL
                     VRERAVIAPGTILSAGVPIHDLVNGRVLRGEVPPGAVVVPGSRPAPGDYAARSGILLA
                     APCIVKYRDAKTDASLALEEALR"
     gene            complement(10236..11174)
                     /locus_tag="P5313_01275"
     CDS             complement(10236..11174)
                     /locus_tag="P5313_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRY79023.1"
                     /translation="MSHALQSAGFRTLFRVSDPRPRDASAPEVSPPRFSRTDFVSPPG
                     QVPPAASQAAPPAQAPHTENKPLRVGIFGRGRLGSAVLENAKDAKGLTVAWALGRGYA
                     PRSPVDVVLDASHADAVRDHVEWAVRTGSDLVIGTSGWTIPDLADLVGDRIGVLTAPN
                     FSLAAALLRRLALVLARFAASYPEADLGITEKHHNKKADAPSGTAKSLAAALVEGCPR
                     FTGWSLAPAAPGKVSVASLRTGYEVGFHEVLLDAPSETMTLTHEARSRDLFARGALEA
                     LRWIRGRKGVYSFDDMAAEMLDPLFRMECADDRGTR"
     regulatory      complement(11267..11455)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(11530..12528)
                     /locus_tag="P5313_01280"
     CDS             complement(11530..12528)
                     /locus_tag="P5313_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRY79024.1"
                     /translation="MTDPAPVSLFRHPAWIAWKESQGWKRAETGLGCSALCRRIGTAG
                     SMAYIPGPLSLPGLRARDIDERGAAMEALSLRALPKLPPDCVFIRWDLMVEAWTDGRG
                     SALNRGLQELRMNASTVRRRFRKAPVEYTCPDTMILDLTGGPAAVWSRMEAKTRYAVR
                     LAERRGTVVERTGEAGLRTFHELYGRTASRKGLPRHPESLFRKFFREARDRGLELDLY
                     LARYKDEPAAAAVVARVRDEAWYLFAASSWNLRSAAGPSAVLHRAAADCARAGVRRMD
                     LLGVSPPDSPDHPLAGLTRFKKGFGGRRHTRAGAWDYVVDGEIYSGYARTEGLSRP"
     gene            complement(12521..12901)
                     /locus_tag="P5313_01285"
     CDS             complement(12521..12901)
                     /locus_tag="P5313_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRY79025.1"
                     /translation="MELYALTDSDYRKLSALALAMRNSRNPGRAAVLDQRLRIARGAG
                     SADSAGIQVTMNSRITIRDEDSGEVFSYRLAFPAEADISTSRISVLTPLGSELLGRRA
                     GESFGYDSPGGRRRVLVEKVVQDD"
     gene            complement(13006..13893)
                     /locus_tag="P5313_01290"
     CDS             complement(13006..13893)
                     /locus_tag="P5313_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRY79026.1"
                     /translation="MATLKERLREREKELACVYSICLLAAGGPEPRDAAEGIARALCA
                     AVQHESRAACVVRFRRIGSEDTVTVRRGPPVPEDGALRPALEAPLPDEDSVGWSGSVR
                     MEYLDPDLSFLPQEKALLDSILVIAASILRTSNLITRLRAASEDLSAKNVALREVLSA
                     IEADRRRTLLAFRERAAAEILPLAERARDRSLTEERRDSYLELLIRELERDVDSIGRG
                     PEGRAALSPREREIAVQVRNGRTSKEIAELLGISRATVERHRHNLRRKLRISDRAVNL
                     AGLLGGMSASAADGFDPYL"
     gene            13980..15170
                     /locus_tag="P5313_01295"
     CDS             13980..15170
                     /locus_tag="P5313_01295"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRY79027.1"
                     /translation="METIRTVFKIGYGPSSSHTMGPARAAESFKERAPEGRYEVTLYG
                     SLAATGKGHLTDEAILKVLGPEVRFEWKPDTILPGHPNALTLEARDEAGRSVLSETWF
                     SVGGGEVTRTPGVRSAESRYDLGRMDGILAWCREEGRNLWEYVAEREDPGIWDYLGGV
                     WEAMGDSIRNGLDHESRLPGPLGVARKASSYFTKSQLQGDPIRVRTRLFAYALAVAEE
                     NASGGRVVTAPTCGSCGVLPAVLYLLEEYYKFSEKRLLHALATAGLVGNLIRHNASIS
                     GAEVGCQGEIGSACAMAAAAAAQLFGGTPAQVEYAAEMGLEHHLGLTCDPIGGLVQIP
                     CIERNAFAAARALDAAEYALLSDGSHRVSFDRVVEVMRRTGHDLPHLYRETALGGLAG
                     DGGR"
     gene            complement(15242..17011)
                     /locus_tag="P5313_01300"
     CDS             complement(15242..17011)
                     /locus_tag="P5313_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY79028.1"
                     /translation="MKKHRNANRTERPVLAAALTAILAAGSAATGLAVPGLGRPAAAA
                     SAALALLAAAVGLRAYVSVRKASRGDAALQAASSDPERGALDLVRLAAAASEDRSLAW
                     HARLLETLNQDIALLQRSAVKFDLFSSDILFSAQTLAGLARRDLEMLTRLRGQVTEFF
                     EEQARTNEELGQLRERLTGSAELAARLARTASDSRSELSLLVDLSREAARDARAGVEG
                     VEATGEAASGLERLLDRLNQAARREAEEADRIAESLARIADIVERTHILATNASIEAA
                     RAGARGSGFAVIAAEVRNLSEASRKALEDIGKVLGSVAAGIRESGALSAQVASHSEQL
                     DRSLSRTRSSFEAIDGRIREVEGRLARFDEVFSEQAEGSNRSSASAGQAASLLEGFAV
                     AFRERTETYEGILEAARESETSVHDAERSARVLAQLAGYLKNGGLDRNRVLRRYRADG
                     DLAQGRLGRRERREELLYNLEIFDPEGASLGHLGDLSPSGLLLVTPGRIEPGTRLALS
                     LALPITAEGERRVPLSVTVRRVEEDGGLFRHGCSIDDRERAAGQVEEILRSLSLGAVS
                     APGLPAAGAGSPGAEEVEEAEEV"
     gene            complement(17058..17795)
                     /locus_tag="P5313_01305"
     CDS             complement(17058..17795)
                     /locus_tag="P5313_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY79029.1"
                     /translation="MRPARGADLGASIPAAFLAAVLVLGMASCGPGTTSPSGAPPSQA
                     PVPASADPEYLPARSRSELPPVVPPKMPAFVPGYAELDPDSGLHMTGTPLELDLKTWR
                     LAVTGAVRRPLSLDFDELRTLPRVRSRSTLVCPGYFEDNAVWAGASLAALLELAEPLE
                     GAKGVNLVSADGYSTWIGMEDARAPHNFLAYEFAEGRPVPVLHGFPVRAVFPSLTGSK
                     WVKWLTEVQVEYADPPKAPPAGSVPGS"
     gene            complement(17792..18151)
                     /locus_tag="P5313_01310"
     CDS             complement(17792..18151)
                     /locus_tag="P5313_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79030.1"
                     /translation="MASWNPSCERWLDATLPQILPVLKRKMFGYPAWFEYGRMRLCLM
                     EGGVGIKSDPGVIRSARDSGIPLGDFVPLGRTAGRTWAFFALPAAEEPWDEESLRFAL
                     GLLLSVPAPERAAGRKP"
     gene            18369..19736
                     /locus_tag="P5313_01315"
     CDS             18369..19736
                     /locus_tag="P5313_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRY79031.1"
                     /translation="MKLKPWIPRESVKIELGRQSRVFLSVTALLGPAYARFVLRLARI
                     GVEGRERLVEEYRRSAADEGRFILAYRHPGDADPHLVFHVLTNLIRGKAPAVEADGRP
                     GAWYPSGSEVQLWASPLVHWALRNAGIVPVRHGAVDRAVLDYLVRAVAERRRPMAIAP
                     EGMATLNGGILPELDPGTTRIALLAAERLASSDKPMPVRIIPLAIEYSYGRTTSPARL
                     GRFLTRLEKRIGLDRAAARPDPGQGATGKDPTSPEAIKSRLLAIWGRLVDIAETTYAR
                     SWGLKPAPTGAGLAERLGVLLDASISRMEAFYGVQPAATLKARILNIRAESLRRVFYS
                     REELAALSPLELAAARRGSAEAYFLDKIYMTAALGQFLDPGYLDGNPSFERLVETAQN
                     LHDFANRLEGLELKHRSKYFLKDARVIAGEPVEASRRPGEGRKEAAERVERELEEGFR
                     RLIRH"
     gene            complement(19741..21060)
                     /locus_tag="P5313_01320"
     CDS             complement(19741..21060)
                     /locus_tag="P5313_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY79032.1"
                     /translation="MAGDTGFGAVEAALDRGAADLEGMAADLAAHPELGFREERTAAV
                     LEKALRETGARVRTRIALTGIRADLGSEDAPAIFLIADLDAILTSGAAGGVAHSCGHH
                     AQMAVMLAVFRALAEAGVPEREKVRLVFLGTPAEEFLEMEYRLGLRAEGKVRFLSGKQ
                     EMIRLGALDDACVALKYHSMSDSPERVATVNGTLNGFLAKRATFLGRSAHAGAWPEKG
                     INALNAASLAMLAIHAQRETFRDDDHIRVHPVMKSGGTTVNTVPDTAVLETYVRGASW
                     PAIRDADAKVDRALAAGAVAIGAAVRIDNSPGYMPFRPDPALGEVLGAAARTVLSERD
                     IDFHDRSFASDDIGDVAALVPTCQLGYAGFSGTIHAADFKPSDPVRAYREPARILARA
                     AFDLALNGGAKALEIRRSFKPEFTKEAYLSSMEALFASRTLEWDPGT"
     gene            21299..22369
                     /locus_tag="P5313_01325"
     CDS             21299..22369
                     /locus_tag="P5313_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY79033.1"
                     /translation="MKRLTLPVVLILALAFALPAAAQTTNVFLLISGSLGDKGFNDSA
                     KAGLDMIKAKYGAKVTTKYTELGADTAKYAPSLEDAGAEGYAVVFCSNNFRSAVQEIA
                     PRYPRTLYVMYDGWVEGDIPNVYSVQYKNSEAGYLAGVLAGSMTRTGGNPKINPDNVI
                     GFIGGRDITGINDFFVGYIAGAQKVNPAVKIVYNYVNSFTDTAKAKDQALIQFGSFKA
                     DVVYHAAGRAGLGLFDAAVETGKYAIGVDTDQASLFPSEPAKAGLILSSTMKRVDLTL
                     AGTFDKFMAGQKLGGQKVYLGVADGVIGFAPLNKAVPGAIQKEMDRIIAEINAGKVAI
                     PSAFSMSAEEINKMRDAVNPNK"
     gene            22579..24132
                     /locus_tag="P5313_01330"
     CDS             22579..24132
                     /locus_tag="P5313_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79034.1"
                     /translation="METILQAVDLCKVYGNGTAANKFVNLTVRRGEILGLVGENGAGK
                     STLMKVVYGEEPPSSGTLLLDGKSVRFGSSRDAIAAGIGMVHQHFMLVPSLTLAENLV
                     LGREPRKKSGMYDLNRAVRIAEELSAKYNLRIIPRARVVDVSVSMRQKLEILKTLYRG
                     ARILILDEPTSVLTPQETDELFAELKALRDKGQTIIFISHKLGEVFQLCDRVTVMRDG
                     AVIATHDIGDVDRQTISNEMVGREVRLTLDKKAAAPGGTALEVRDLSFVDDFGITMVD
                     RVSFSLRKGEILGVVGVDGNGQSELVRILTGHARTLTGSVRKGGTDLTNASPRKVRKA
                     GMAHVAEDRMTVGTALPASIADNLLADRYSEPAFTGGGFLRRSAIQAEAKGIVQRFDI
                     RCSSPNQAVASLSGGNMQKVVIGREFTAAADVLVVSQPTRGVDIGAIEFIHRTILSMR
                     DEGKAILLVSSDLGEVLSLSDSLIVMHSGKIVAWFPDASAVTDKELGLYMLGLKTQDE
                     IEIRKVLHE"
     gene            24125..25270
                     /locus_tag="P5313_01335"
     CDS             24125..25270
                     /locus_tag="P5313_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79035.1"
                     /translation="MSDGTRTKTLPGKVGLFFTNRDALEPALVTLFAAVFSLLAGVGL
                     IFLVSKEPAQSARNFLLGPFLRTYNIYYMMIAMVPLTFTGLALCIIYQSKHMSLIVDS
                     SFYIGAVVATAAGIHVALPAGWHPAFAMLAAGITGGFIGLLPAILKIRWRANELVSSL
                     MLNYVFYFVGSFVILYFLRDRNQTILSSLPFRNTFLLPRLIPNTQLHAGFIVAAVFVV
                     LVSVFLYRTKWGYEIRMIGANQKFAAYAGLSVPTVILYSHFVSGFLGGMGGAIEMAGL
                     YKAFIWQMNPSYAWDGVIVAMLARKNPKFVPLSAFFLAYMRVGADFMSRRGDVAFEFI
                     TLLQGLIILILASDKIVNYFKARRLQRTALARTAEARAAGAPAGGAE"
     gene            25267..26220
                     /locus_tag="P5313_01340"
     CDS             25267..26220
                     /locus_tag="P5313_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79036.1"
                     /translation="MMEFLASVFGSSNYWFIVVRSTAPILLVTLGAVITEQAGIKNMA
                     LEGSMLWAALTGVLASAWTGSPWIGLAAGVAAGVAIALVLGLFALKLDANAVLVGIAL
                     NLLSTGGTVFVLFSITGDKGISSSLPSKVLPMVSVPLLRDIPWLGKVLSGQNLIVYLA
                     AAAVVVIHVILNKLTIGRRIRATGENVEAARAAGIDPDRIKFFTLIVSGVLAGLAGCF
                     LSMGYTSNFTSGISAGRGYVANAAQVVAGGTAVGSIFAALIFGVTDALATFLQSRGLP
                     PELIQALPYAVTILGFVVVTLVRDRREAARKLRLFAGRDDR"
     gene            26217..27545
                     /locus_tag="P5313_01345"
     CDS             26217..27545
                     /locus_tag="P5313_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRY79037.1"
                     /translation="MTCDLLIENAAVLTVDAAWNLYEPGFLAVKGDSIAAVGPMAVRR
                     AWEARTVVDAAGKLLMPGFVNAHTHVAMAAFRGACEDVDDRLRKYIFPLEARLVDRDL
                     VYRASRFCLVEMAASGTTCFGDMYYFEDEVARAAREAGLRCVLGETVVDFPAPDCPEP
                     YGGLGYGLDFISDWAGDALVKPCLAPHAPYTVDAEHLRRVRRESEARGLPVLMHIAET
                     APENERFTASHGSVLRYLDAEGALYPGLVGAHMIYLDPSDIALAAARGLSVAHCPGSN
                     AKSGRPICPAADLADAGVPVGLGTDGPLSGNGMDLQGIVGLFPKLQKMRRNDRGAVPA
                     RQAVRAATRGGAEALGLGKVTGSLEPGKKADLVLVDADSFGLQPVYDWYAAVVYSMRP
                     SDVAATYVGGRLVWDRTGMKGFDQAEAMADVRGVADRCRGVIAELTGGAS"
     gene            27542..28276
                     /locus_tag="P5313_01350"
     CDS             27542..28276
                     /locus_tag="P5313_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRY79038.1"
                     /translation="MTESTPHIVPEGPIAPSVLMPGDPLRAKYIAERFLEKAVRFNSV
                     RGMLGFTGTWRGVQVSAMGSGMGIPSMGIYSYELFSAFGVRDILRVGTCGGFREDMEL
                     GDLVFAQASSTSSSWAEQYGLGGTFSAIPDFGLLEAGVAAARDLGRRFVVGNTVCLDQ
                     FSRYHAVDKPWTPWARMGCVATDMEAYALYCNAAYLGRRALAMFAVSDLRLKGLHMES
                     ADRERSLDSMIETALETVRRVRAAEA"
     gene            complement(28291..29655)
                     /locus_tag="P5313_01355"
     CDS             complement(28291..29655)
                     /locus_tag="P5313_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRY79039.1"
                     /translation="MTDHIWNYIEGRAGEYDDLARDIWEHPERSGEEERSAALHRDRL
                     SASGFRITIYPEMPHAFAAERGAGKPVIALMGEYDALPGLSQACSVRREARKPGAPGH
                     ACGHNLLGIGSLAAAEALADALAAAGLPGTVRYYGCPAEEALGRIPQVKAGRFDDADA
                     ALTWHPADVNTPHRYTTSANVALVVRFAGRASHAAMAPHAGRSALDGAQIFNLGLEFL
                     REHVERGVLIHYVISDGGARPNIVPESAAVTLYVRAPTAQALRLAVARVLKAAKGAAM
                     MTETRVSWEVSSGKCDYIPNDAIHGAMLGAMRRLPVPALAPQETAFARDLAATVPKRD
                     RESTLTMIGAPLSLLKRPTHDQAGDFGEGFRIGGSLDTGDVSYVTPTGQMNAATWPLG
                     VGAHTWQSCSASGSTGAFKRMRWAAAVLAATGFALASDPALLAAARAEHRRKTRPYRS
                     TMDL"
     gene            complement(29652..30206)
                     /locus_tag="P5313_01360"
     CDS             complement(29652..30206)
                     /locus_tag="P5313_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRY79040.1"
                     /translation="MTRIRLSARTAAALAAAALAVLTSCSGAPSTKGIPAVRSIDFGL
                     FEGAWYEIARIPVPVARDWVGTTDTYIPNDDGTWRVLYEGYKGGFDGKKGVMRQKLRI
                     PDPAVAGEMSARPFPFTWLPYRLVYWNEADRTMLVTSGTMDFLWLMAKDPVPPPEVYE
                     GLVEESRRLGFDVSRLERVPQGRP"
     gene            complement(30203..30991)
                     /locus_tag="P5313_01365"
     CDS             complement(30203..30991)
                     /locus_tag="P5313_01365"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRY79041.1"
                     /translation="MNPFVLSAVAAVAINGIFFAYAALRKTDVVTDLSYSLSFALVSA
                     VLILRNLGASPAALVPAVLTLLWAARLGSYLFTRIIKIKVDHRFDDKRDDPWKFASFW
                     SLQAVAVFVILLPVVASIASSVSPRSFGTFEIVGMVLWLVGFVIEVEADAQKSAFKKA
                     GRSGVITTGLWSWSRHPNYFGESLLWWGLFLYAVPALSGFLWISVLGPVFITVLLLFV
                     SGIPLLEKSADAKYGSDPAYQEYKRRTSIFLPLPPRPAEKGTSK"
     gene            complement(31064..31672)
                     /locus_tag="P5313_01370"
     CDS             complement(31064..31672)
                     /locus_tag="P5313_01370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY79042.1"
                     /translation="MHPDSGLSHAERHARLFERIARPYAWFFHGQLRTYARAFELGRS
                     FLPDPAGRRALDIGCGTGAFTRALAREGWEVRGVDVARAMLERARSRGLSCDPGDALR
                     GLAEPDGSFDLVTAAYVAHGLRREDRAKLFAEMRRLSRDTVLFQDYTPDRSLLVTIVE
                     YLEHGDYFNFIRTGLPELREAFPSVQVVRVGPRAAWYVCKAA"
     gene            complement(31775..33463)
                     /locus_tag="P5313_01375"
     CDS             complement(31775..33463)
                     /locus_tag="P5313_01375"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRY79043.1"
                     /translation="MSIYNIFDLAGGLAIFLFGMVMMNNNLTALAGARLRTIMIELTK
                     TKVRGYLTGLGVTIVNQSSSATTVLEAVLVGAGLMTFRQSLAVTLGAELGSTFLGQLF
                     AFPKITRLAALFVALGFFAFLLASKKQHKSAANTVLGFGLLFLGMDMMSRSMEPLRTL
                     PAFLNLMASVERPLIGILVGLVFTMIVQSSGATSGLVIAMAISGTISLAQAVPINLGA
                     SIGTCVTAVLGSLSLNREAKRSAYIHVVFQTIGVILAYILLSIPIQGDRLYLFLAREL
                     SLLFTGGRENLAREIAMAHTLMPVLNHILVIPLLPLIVKAFDRLVPPEPPKEVFGPQY
                     LSEAMLTEPSVALFQVKKEILRMRPIIEHMLEASVRLFSERRPEDGKAIKTEDKYIDT
                     LRTEIVRYLTKVAKQSLTESESKRQVSYLFIVSELENLADVIEGNIRDRAKKLINNDL
                     RLSDEGLADVQELAKVVADNFRLVMRAFEDNDTGGARQVLECSESCWELQREFRRKHF
                     RRLNEGVQVSIETTEIHMDLLNHLHRVNRHIYHVAQALLELADEPVPDQHRSAM"
     gene            33642..34601
                     /locus_tag="P5313_01380"
     CDS             33642..34601
                     /locus_tag="P5313_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79044.1"
                     /translation="MDDLLERALEDGPLLRSRVEAFLGPAPEKVPDFWKPGIGEIHYV
                     PVPGAEIRIIQVRPDRTAARRPVVLVPGWGTTPEGFREFYGPLHGKAEFLFIETREKN
                     SSRIQGRKPDLSVERAALDIRAALEAAGLLDRDYVLAGTCWGSAQILEGMIRGYLDPS
                     TVLLADPMHTLWFNKTFLRRVSPWIPAAAIRAVRPLLRQALVGDLKEGAQKQRTFEFI
                     DRADVWKWKKSAEAAWDFELFGRLGAVRREVFVLNGTGDKVHDPRNYPRVAAELPAGR
                     FLRMPLDESLRERMFGTVALEFARGRAGEGLPARLAEFERAVR"
     gene            complement(34631..35581)
                     /locus_tag="P5313_01385"
     CDS             complement(34631..35581)
                     /locus_tag="P5313_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79045.1"
                     /translation="MVGMNKVFRAARVAAAGLALSLVLTTCEPLALRDLAEKLAQGGA
                     KHLYITDRDAKRIYRCETDGRNLIEILPLSNQDDLAMIAVDPNAGKLFWTCAAISNLG
                     KVVSSNLDGTGLSVLYQEPGPIGGVFPRGLSPNIAEEVLFWAGISNDTIYRQPYSSAE
                     SPLAGAGIGDPQDLAYYPNLDKVFVADPANKKIHRINSNGSLDLTLDLTPVSAGFKTP
                     GNLIVDNLHGYVYWSDDGPGGGIFRCDANLSSASVTNIVVWAGAPPRQLAVDPDEGFL
                     FWVDLGTKTIQRAKLDGSDVRTIVSGLQGPRGVALDPVRR"
     gene            complement(35569..37680)
                     /locus_tag="P5313_01390"
     CDS             complement(35569..37680)
                     /locus_tag="P5313_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79046.1"
                     /translation="MRRFRLLRPLPRWLLLFILIVSAVSADETRPWSLSAGPGVEAPL
                     GNGASVFGLGVGVRAGLVRNRGPFGTAPGLWLDYTLQPVGEYSISNLSAALDLALPFR
                     LSDAIRLSPYLAGGVFFSFFNQRSELEAAFPDDTFAGIGPWAAGGLRLGWTFPKGYTA
                     VLDLGYRGRFGLSHGLGLGVGVSYGLRDRRGSALPQGMRLQPLSTVSFRDIRLDPVFP
                     VFYKHYDQNPVGTATLVNSGKQEITDIKVSLFSRQFMDDPKTCSGPRTLAPAASAEYS
                     LLALFNPLVLELSERTKASVVLAVEFSSGGIRYRNEYVESLGFEDRNATVWDDDRKAA
                     AFVSVKDPDVLTWARRASEASETPGSAVDRNLAAAVAAFEILRLASFRYSVDPTTPYR
                     EFSTRSGAVDYLQFPRQTLRLRSGDCDDLTVLYAALLESVGVGTAFVTVPGHIYTAVA
                     LDTTPAGMRKTFRSTSDFLEASGRAWVPVEVTALDLGFDEAWHLGARQWREQGAAGKA
                     RLWPVAQAWELYEPVGLARGEPVPEPPSADKVRAAASEVWRRLLEREYRPQVAGLEKA
                     ALSGGPKGYNALGILHARYGFLEEAEKAFQAAIKAEAYPPALVNLGNLALLRGRVEEA
                     YRHYDRAAASAPKNPAALLGLARASQLLENYGTARRAFDQLKAVDPALASRYEYLAQA
                     GQENARAAEAQRARRVDTWSE"
     gene            37988..38965
                     /locus_tag="P5313_01395"
     CDS             37988..38965
                     /locus_tag="P5313_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY79047.1"
                     /translation="MKRRFILLAVLLATVGASILWAAPVVLKLGHGAQATHASHIAGL
                     RFAELVKERTKGQVEVQVFPNRQLGEERDLVEGLQIGTVDFAVVSTGPLGGFVPEVGV
                     VDLPFLFTTAAHAYKVLDGEIGRGILDKFQSRGIIGVAFWENGWRNLSTSKRKVLKPE
                     DLKGLKIRTMENKVHMASFTAAGASPVPMVWGEVYTSLQQGVIDGQENPVVIIHSNAL
                     WEVQKYYALTGHFYGSHVFLASKRNLDKLPKDVQKIILDTARELSAFQRDYSKKLEDE
                     LIVQLKAKGMDVYEIPNKKAFQDAMQPVYKQFESQFGKALIDKILAAGR"
     gene            39049..39531
                     /locus_tag="P5313_01400"
     CDS             39049..39531
                     /locus_tag="P5313_01400"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY79048.1"
                     /translation="MDAFILGLKKAIDRIVDAVAVSALSVLVLVVAAQVISRYVVGRP
                     FTWSEELARYMLVWIVYLFAVIAFRLSRHMGIDFFVLLLSDRFRGIVQGAGDVLIFGF
                     LILVLRFGTELVGITLPQTSAALGIPMGLVYLAFPVSAGLMALELLFRLYSRLTGGRR
                     "
     gene            39528..>39990
                     /locus_tag="P5313_01405"
     CDS             39528..>39990
                     /locus_tag="P5313_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY79049.1"
                     /translation="MTTTLFLSFAVLILLNVPIAFSLFISASLALALNGITLMAVIQR
                     GVTAVDSFILLAIPFFMLAGNLMSGGGISNRIIKFANSSVGFIRGGLAHVNIATSMLF
                     AGITGAAVADTSAVGSVLIPAMKDEGYDADFSAAVTASSSVIGVIIPPSIPF"
BASE COUNT         6219 a        13577 c        13954 g         6240 t
ORIGIN      
        1 ggtcccgtac tcgccccggt aggcggagtt gccgatgaag gcgctgccga ggatcccggg
       61 tagacggccg atcccggaga tcaggatgaa gaggggcagg gagatccggc tcatcccgac
      121 gacgtacgag agggcgtcct tggggatgct ggggatcacg tacagcagga agaagcccgc
      181 ggcctcccgg ccggaggcgg ccgccttttc cagccgcgcg aggacctcgg gcttcacgag
      241 gcgttccacg aagggtcgtc cggccaggcg gccgacgtaa taattgaaga ccgagccggc
      301 caggatcccc agggacgaga ggatcgtggc cccccagaac ccgaagacgt agcccccgcc
      361 gatctgcacg acctccccgg gaatgatgaa aaccacgacc tgcaggaact ggagcccgac
      421 gtaggccagg ggcgcggccg cgcccaggga ctcgacccag gcccgggccc gctgggggtc
      481 ccggaagatc ccggtcaggg gctgccagag gaggatgcct ccgcccaccg cggcggccag
      541 gatgagggga aaggcgagag cggcgatccg ggagccgggt ttacgggact tgtccatggc
      601 caggagtgta gccggggggc ttcggtttct caaggtatac gccaaatgca taaaaatgcg
      661 tataagctct tgacggaacc ttccgatatg gaataggatc tcgtcagata ctgattccgg
      721 gaggttccca tgtcctgctg tcccgaaccg tacgggctct cattcctcgc aaagacccct
      781 gtttcccctt ccatccgccg tattttcccc cgcctccgtc gtcgttaacc gaaatccccg
      841 atagacagtt cccataggtt cgagatccgg acgaggcgtc cggggaggtt ccattcccgc
      901 gccttcgccg ggaacgtatt tttatgcaaa ggagtcgcat atgaagatag ccctggccta
      961 ttccggcgga ttggacacga cggtcatcgt gccctggctc aaggagacct acgactgcga
     1021 ggtgatcgcc gtctgcggcg acgtcgggca ggactgcgac tgggcggacc tggagaagcg
     1081 ggccctgtcc tcgggggcct ccaagctcat cgtgaaggac ctgaaacggg agttcgtcga
     1141 agactacctc tggcccctgg tgaaggcggg ggccgcctac gagggccggt acctcctggg
     1201 caccgccgcg gcccggcccc tcctggcctc tagccttacg gagacggccc tggcggaggg
     1261 ggcggacgcg gtcgcccacg ggtgcacggg caaggggaac gaccaggtgc gcttcgagct
     1321 ggggatccgc gccgcggccc cgcacatgga tatcatcgcc ccctggcgga tctggaacct
     1381 ctccagccgg gaggacgaga tcgcctacct ggaggcccgg ggcatcccgg tccccgtgaa
     1441 ggtggaggat tcctactccc gggacgacaa cctctggcac gtgagccacg agggcttgga
     1501 cctggaggat cccgccttgg aacccaagtg ggaggggatg ctcaaactgt gctgtacccc
     1561 ggagaaggct ccgaacgtgc cggagtacgt cgagctgggc ttcgaggggg gcgtgccggt
     1621 ggccctgaac ggggtccgga tggggggcgt cgagctcgtc tcggccctga acgtcgcggc
     1681 ggggcgccac gggatcggcg tccttgatat ggtagagaac cgggtggtgg gcatgaagag
     1741 ccggggtgtc tacgagactc ccgcgggcac ggtcatcctg gaggcccacg ccaagctgga
     1801 gcagctctgc ctggaccgga agaccctttc cttcaagctc caggccgccc agcgcttcgc
     1861 ggagctcctc tacgagggcg agtggacgag ccccctgatg gaagcccttc gcgccttcgt
     1921 ggattccacc cagaaggtgg tcaccggaac ggtgaagctc aagctctaca agggtgctat
     1981 actgggggcc ggcgtcgcct cggcgtactc gctgtacgac aagtccctgg cctccttcgc
     2041 cacggggccc ctgtacgacc accgggacgc gacgggcttc gtcaacctga ccgccttgcc
     2101 gaccaaggcc cggagcctgg tgaactcgcg cctggcggcc cggaaggcgg ccgcgggcgg
     2161 cggggacccg accttccggt acgggaccgg gcgggtggcg gcctcggcgc cctaaggcgc
     2221 cggccggaaa aggagcatcg atggccaagc tctgggaagt cggatcgtcg ggcctggacc
     2281 cgagggtgga cgcgttcctg tcctccgtgg ccgtggacgg ccgccttttg gccgaagacc
     2341 tggagggctc gatcgcccac gcccgtatgc tgggcaagca gggcatcctt cccgcggctt
     2401 ccgccgaggc cctggtgtcc gagctggagg ccctccggtc cgaggcggcc tcgggctccc
     2461 tggtcccgga tcccaaggcc gaggacgtcc actccttcgt ggagggcgag ctgacccgcc
     2521 gcctggggga cgcaggcagg gccgtgcacg cggggcgcag ccggaacgac caggtggccg
     2581 tggacctgcg tctcttcctc aagaaggcct ttgcccgggc ccggtccggg agcctgtcgg
     2641 cgatctcggc gatcctggac ctggccgagg ggcatacggc caccctcatg cccggctaca
     2701 cccacctcca gcgggcccag cctgtgacct tcgccttcca cctcctggcc tggtgcgccg
     2761 cccttgagcg ggacgcgggc cgcttcgcgg acgggcttgc ccgggcggac gagtgccccc
     2821 tgggctccgg ggccctggcc ggctcgggcc tgcccctgga ccgggaggcc acggcgaagg
     2881 acctgggctt ttgccggcct tcccggaacg ccatggacgc caccgcggac cgggacgcct
     2941 gcgtcgagta cgcggccgcg gcctccacct tgctcatgca cctctcccgg ttttgcgagg
     3001 aggtggtcct ctggtcgtcc gaggagtttg gcttcgtgaa agtggggccg gcctgctcca
     3061 cgggctccag catcatgccc cagaagcgga accccgacac cgccgaggtg atccggggca
     3121 aggcggggag ggtcttcggg ggcttggtga gcctgctcgt cctccagaag ggtctgcccc
     3181 tggcctacgg ccgggacctc caggaggaca aggaggccct cttcgacgtg gtggacaccg
     3241 tggacgggtg tctggagtcc ttcgcgatcc tggtgaagtc cctggagccg aacctggagc
     3301 ggatgcgccg ggccgccgaa accggcttcc tggaagccac ggacatagcg gaactcctgg
     3361 tgctgaaggg catacccttc cggtccgcct acggggcggc caagcaggcc gtcctcacct
     3421 gcctctccct ggggctacgg cccgcggacc tttccaactc cgacctggcg gggatctccc
     3481 ccctcttcgc ccgggcgggg atccgtgcgg aggaactggc ggcctacctg gacctggacg
     3541 cctgcgtcgc ccgccggaac gggaccggag gccccgcccc cgagcggacc cgggacgaga
     3601 tcgcccgcct gcgagccttc gcggccgggt ggtgagggcg ccatgttcga ctgcatcatc
     3661 cctgccgcgg gggcttccag ccgcatgggg gcctggaagc cgggcctcgc gttccgggac
     3721 gggaccctgc tgtccgcggc cgccgcccgg gccctggagg cgggctgccg cgtgatcgtg
     3781 gtggccggcc gggccgcgga ctcggtcccg ggagccctgg gaagccgggg ggaccgggtc
     3841 gagatcgtcc tgaatcccgc ctgggaggaa gggatgatgg gctccctcca ggcgggcatg
     3901 gcccgggtct cttccccctt cttcttcgtc ctccccgccg acatgccctt cgtcccggcc
     3961 cgggccttcc ggctcctggc ccgggaagcc gaagtccggg cggcagccgg gcttccggag
     4021 agtccggtct ttccggcctt ccggggggag cctggccacc cggtcctgat cccctcttcc
     4081 ctgatccggg aggccctgcg ccttccccgg gacgcccggt tcaaggactt cctggaagcc
     4141 cgaggcccgg tgtacgtgga agtcggggat ccgggaatac gcacggacct ggatacccgg
     4201 tcggagtacg agggcgccct gaggctcgtg tcccgaagct cgtgaagccc gcctcctatc
     4261 cttgacaggg atattcatgc cccatatatt cacgatgtga atagggggtg ccatgaacct
     4321 gtcccgctcg cgttccgtcc cggttgccgc tctggtcctg atcgtcgcgg cctcggccca
     4381 ttcccaggtc ccgccggccc gcatcatcat gggcggccgg gccgcggtcc tggtggcgga
     4441 cgcggtgtat gccttcccct cggcccggtc ccgggtcatc accgtggggg gcggggacca
     4501 gggcctggga accttcctcc aggagctgga tccctcctac ccgtcgaagc cggtcctgga
     4561 ccggaatgcc gccgcggagg tctatgccgc ctaccgtccg gacctggcga tttttaaaag
     4621 tgccctgaag aggggagtgg gcggcgccct ggaatccttg ggtatccggg tccactacct
     4681 ggacctggaa accccggaag actattaccg ggaccttgaa ggcctgggcc ggatcctggg
     4741 ggagccgtcc cgggccgcgg aactggtcgc ctactataaa ggggtcgtgg agacggcggc
     4801 ccgaatctcg gcgggggcat cccggaaacc ccgggtcctg gtggtccagg ccgtcgggga
     4861 tgcctgggag gttccccccg cgtcctggat gcagacccgg atcgtggaga tggccggagg
     4921 cgtaccggtc tggatcgggg ccaaccccgg ggagggctgg gcccgggtgg gcctggagca
     4981 ggtcgcggcg tggaacccgg acgtcctcct ggtcatcgat tatcgccggg gggtggacga
     5041 ggccgtggcc cggatgcggg cggacccccg cttttcgctc ctggccgcgg gaaagtccgg
     5101 gatgatccgg ggcttccccc aggacttctt cagctgggac cagcccgata cgcgctgggc
     5161 cctgggcctc ctctggacgg tcaagactct ccacccggac cgctcgggca ccctctccct
     5221 ggaggccgag gcgcgacgct tctacagggt cctctacggc ttcgacgacg cggccttcga
     5281 ccgggcggtc cggccccggc tgaccgggga tcatggcatc cggtagcgcc gacgccccgc
     5341 ggccggccgg gagggtccgg gcggcgggtc tgtgggccgc agccttcctc ctcttcgccc
     5401 tggccctcct ggccggtcgc tatccgaacc cgggcttcct ggacctccgc ttgctggcct
     5461 ccgacgacct ggcccggacc atcctgctaa gcgtccgcct acctcgggtc ctggcggccg
     5521 ccctcctggg cgcggtcctg gccgcctcgg gcaacgcctt ccagatggtc ttttccaatc
     5581 ccctggtgga accgggcttc ctgggggtct cccagggcgc ggccttcggt gcggccctgg
     5641 ccttggttct gggcttccgg cacgacctgg ccgtctcggg ctccgccttc gtcttcgcgc
     5701 tggcgggcct gggagcctcc gcggccctgg cgcggcggtt ccgcttcggg ggctgggtcc
     5761 tgaggctggt cctggcgggc atcgcggtgt ccgccttctt ctcctccgcc ctggcctggg
     5821 tcaagtacgc cgcggatccc ctgcgggagc ttccggacat cgcctactgg accctggggg
     5881 ggctctcggg gatcgggtgg aagaggctgg ccgtggtcgc gccgatcgcc ctggcctccc
     5941 tggcggcgct ccgggcggcg gggtggaggc tgtccatcct gtccctggag gactccgcct
     6001 ccttttccct gggcgcccgc ccgggccggg agcgcgccct ggtcctggcc gccgccgtgg
     6061 ccggggtggc gtccatgacg gcggtcagcg ggatcgtctc ctgggccggg ctcatcgtgc
     6121 cccacgcggc ccgcctcctg gtcggtgcgg acgggcggag atccatgccc gcctccatcg
     6181 ccctgggggc cgccttcctc atggcctgcg acgccctgtc ccggtccctc ctccccgggg
     6241 agctgcccct gggggtaacg accgccttcc tgggcaccgc gggcttcacg gccctcctcc
     6301 tgggccgggt cgtgacggtg gtccgctgat gctggaatac cggcaggttt gcctgcgcta
     6361 ccccgagggg gaccgggacg cggtggccgg gttttccctt tcggtgcccg aggggagcgt
     6421 acgggccctg ctcgggccga acggctccgg aaaatcgacc ctcctgctgg cggccctggg
     6481 ctggatgcgc ccccgggcgg gggaggtcct actggccggc cggccggtct ccgcctggtc
     6541 cccgggggag cgggcccgga aggcggccca cctctcccag atagagcggg tctccttcac
     6601 ctacgaggtc ctggagttcg tcctgatggg ccgggctccc catctggccc ccctggccga
     6661 gccggacgag ggggacgagg agcgggcccg ggcggccctg gacgccctgg ggatcgggga
     6721 cttcgcccgc cggcacgtca cggccctgtc cgggggggag ctccagctcg tccgcctggc
     6781 ccggtgcctg gtccaggagg ctcccctggt cctcctggac gagcccaccg cggccctgga
     6841 ccccgccaac gccctgcggg tggcggacgc cctggcctcc ctggcccggg agggccgcac
     6901 ggtggtcttc tccacccacg acgcggccct ggccgcctat gcctcggatt ccgtagtctt
     6961 gatccgggag ggacggctct tggcttccgg cccgtccgcg gagatcctga cccccgaact
     7021 cctgtcgcgg actttcgccg tgcccttcgg ggtctccggg gcgccctcgc ccttcgtcac
     7081 gggatccagt attcgcgcgg cagggggtgg cagaggaagc ggctcatggc ctcggtgaag
     7141 ccgtaggccc cggtacgccc gaaggccacg aaatcccccg agtccaggcc gggtagaagg
     7201 acgtccccca ggcggtccag ggaggtgcac agggggccgg tcaggacggc cggtccggcc
     7261 tcttcctcga tggaggatcc gtccgcccgg aaggcccgga ccgggaaggg ctggccggtc
     7321 aggaggggcc ggaggaggtg gttgatcccc ccttccagga tcacgaagcg gaccccccgg
     7381 ctttccttga cccggaccac ccgcgccgcg tagaccccgc agggtccggc cagccagcgt
     7441 ccgggctcca ggaccagttc gccccggaac cagtcgtgcc gttccaggag gctcccgagg
     7501 gaacggccca gggcctcgac atccagctcc tcctccccgg ggctataggc gagcccgagg
     7561 cccccgccca ggtcgatctg gcgcaggtca aggccgaagg cttccgccgc cttccgtgaa
     7621 agccccagga cggtgtcgta ggttcgggcc agtttgtccg catccctctc gttgctggcg
     7681 gcgaagacgt ggatgccctc gatccggacc cgggtgaagc gtttggcgac gtgcaggacc
     7741 cgggccaggt tttcctcgtc caccccgaag gcggaggggc cggtcccccc gatgatccgg
     7801 gaaccctcct cgatacccga ggcggggtgg acgcggaggt tgaccgccgc gggtttccgg
     7861 gagaaccggt ccaccagggc caggtcctcc acgctctccg cgtggatccg ggcccccagg
     7921 cgcagggccc ggcggatctc ctcggcccgc ttgcccgggc cgccgtagaa caggaggttg
     7981 ccgggccccg ggccgcccct accggccgcg gtggtgcggg ttccggcgag ggggcgggcc
     8041 ttggccaggg cccgggcggc ccggtcgagc tcccccccgg aggcgcagtc gaaggcggca
     8101 cccttccggg cgaaggtcgc gagcaggccg ggattcgggt tggccttgac cgcgtaggcc
     8161 aggcggaccc ggggcggcag gacggcggcc aggcggtccc agcgttcccg ggcggcggaa
     8221 cgggaatacg cgaagaaggg ccccggggcc cgggccgcca ggtccgcgag ggtttggtcg
     8281 gatacggcga agaagctcac tggtccctcc agaaggcgga gaggcggcgg acgccttccg
     8341 tgatatcgac gggagatccc gcccaggata cccgcgcgta ggacgcgccc cgagtcccga
     8401 aggccgagcc gggcacgacg atcaccccgc cccgatcccg gaccgcaagg cagaaggaca
     8461 gggggtccgc ccggcccgcg ggaggcaggg caagccaggc gtagaagcct cccgcggggg
     8521 ccgggggatc ccagcccagg tgttcccggg ccgcggagcg taaagtttcc cggcggacgg
     8581 ccagttcgga ccgggcccgg gggaacaccg atcccgcgga ggcgatgagg gccgcggcgg
     8641 ccgcctgggc gggccgggac aggcaggaga ccatggcgtt gtgcacgagc cgggcggggg
     8701 ccaggacctc cggatccccg tgcgcccagc ctagtcggag ccccggggcg gcaaaggcct
     8761 tgctcaggga gcccaggacg acggccccgg ggcggatccc cgtgtccgcg agtcccgcgg
     8821 ggcgctcgtc aaggtagagc tcccggtaga cctcgtcgga gaccaggacc accccgcggg
     8881 cccggcaggc cgcgtagacc tcgtccaggg ccccccggga agccccggcg ccggtcggat
     8941 tggcggggtg gttcacgatc gccagccgcg tccggggcgc ggagtccaaa acggcccgga
     9001 agagccccgg atccagggag aagtccgggg ccagggggta ggggaccggc tcggccccgg
     9061 ccagccgggc cagggtcggg taggccagga agcccgggtc cggaaggagg acctggtccc
     9121 ccgggcccgc ccaggcgtgg acaagggaaa atagggcacc ctggctcccc gcggacacga
     9181 ggaactcggc atccgtggcg cccaggaaat ccctgaagac ggcccgaagc tccggggtgc
     9241 ccgcgttggg accgtagggg caggagccct ccgaactcgc ccacgcggcc agggcctccc
     9301 gggcttcccg gggaagttcc caggagggtt cccccaggcc cagggaaacg cagccggcgg
     9361 gcgccccctc gttcagggcc cggatcacgg agagacccag gccctccatg cgggcggcgg
     9421 gtctcaccgg agggcctcct ccagggccag ggaggcgtcg gtcttggcgt ccctgtactt
     9481 cacgatgcag ggggcggcca ggaggatccc cgagcgggcc gcgtagtccc ccggggcggg
     9541 ccgggagccc ggcacgacca cggcccccgg aggaacctct ccacgcagga cccggccgtt
     9601 caccaggtcg tggatgggca cccccgcgga caggatcgtc cccggggcga tgaccgcccg
     9661 ttcccggacc aggaccccct ccaggagggc cgccagggct cccacgaagg ccccgtcctc
     9721 caccaccacg ggccgggccc ccgcgggctc caggaccccg ccgatttggg cccccgcgga
     9781 gaggtggacc cgttccccga tctgggcgca ggaccccacc agggcgtggg agtccaccat
     9841 ggtcccgtcc ccgatccagg ctcccacgtt gacgaaggcc gggggcatga ccacgacccc
     9901 ccggcccagg cgcgctcccc gacggaccga ggagcccccg gggaccatgc gcaccccgtc
     9961 ggcctcggag atctcccggg gcgggtagga ggccttgtcc cgggctcccc cgggccaggc
    10021 cgggaaggag atcaccgggg cggccttgaa gcccgccagg atgccccgct tgacccaggt
    10081 cacggcggtc caggttccgt cggggccccg gaacgcggcg cgcacctccc cggactccag
    10141 ggcggccagg aaggcttcgt ggaactccga ccatccgggg cgggcggcga tctcgtccgg
    10201 ggtgccctgg cagaaggcct cgaggtcggc ggggctcacc gtgtcccccg atcgtcggcg
    10261 cactccatcc ggaagagggg atccaacatc tcggcggcca tgtcgtcgaa ggagtagacc
    10321 cccttccggc cccggatcca ccggagggcc tccagggccc cccgggcgaa gaggtcccgg
    10381 gaccgggcct cgtgggtcag ggtcatggtc tccgaagggg cgtccaggag gacctcgtgg
    10441 aaccccacct cgtaaccggt ccgcagtgag gccacggaga ccttaccggg ggcggcgggg
    10501 gcgagggacc agcccgtgaa ccggggacag ccctccacca gggcggccgc cagggacttg
    10561 gcggtgccgc tgggggcgtc ggccttcttg ttgtggtgct tctcggtgat tcccaggtcc
    10621 gcttcggggt atgaggcggc gaagcgggcc aggaccaggg ccaggcggcg caggagggcc
    10681 gcggccaggg agaagttcgg ggccgtgagg accccgatcc ggtcccccac gaggtccgcc
    10741 aggtccggga tggtccagcc cgaggtaccg atgaccaggt ccgagcccgt gcggacggcc
    10801 cactccacgt ggtcccgcac ggcatcggca tgactggcgt ccaggaccac gtccaccggg
    10861 gaccgggggg cgtagccccg gcccagagcc caggccacgg tcaggccctt ggcgtccttg
    10921 gcgttctcca ggaccgcgga gcccaggcgt ccgcggccga agatcccgac ccgcaggggc
    10981 ttgttctcgg tgtgaggggc ctgcgcgggc ggcgcggctt gtgacgccgc gggcggtacc
    11041 tgtcccggag gagatacgaa atccgttcgg gaaaaccgcg ggggagaaac ttccggggcg
    11101 gaagcgtccc gcggtcgcgg gtccgaaacc ctgaataggg tccggaaccc cgcggattga
    11161 agggcgtgcg acatggcacc tccaatccga gggaggtccc atgggcgaac gcgaatccca
    11221 taccctatgg gacggaccca tagcgcattc gtcgggacaa gcgatgcatc cctctggcac
    11281 tccgcgggcg gctttggttt tgcccgcgac agccgccggg tattagcccg gacgtccaag
    11341 tcggcccggg aacggccccg gaccttcctt cggcgatcct cccctttcgg ccggggtcta
    11401 cgggatccgc tcccttgccc ggcgtactcc tggggatcgc tcctccatcg gtaggctgga
    11461 ccatagcacg gggaagggcg aagcgcaagc ggtgcggccg cggtttgcgg ggacgggcgg
    11521 ccttcgcgct cagggccgcg aaaggccttc cgtccgggca tagccggaat agatctcccc
    11581 gtcgaccacg taatcccaag cgccggcccg ggtatgacgg cggcccccga agcctttctt
    11641 gaaacgggtc aagcccgcca agggatggtc cgggctgtcc gggggactca cgcccagaag
    11701 atccatacgg cgcacccccg cccgggcgca atccgcggcg gcgcgatgga ggacggcgct
    11761 gggccccgcg gccgaacgca ggttccagga ggaggccgcg aacaggtacc aggcctcgtc
    11821 ccggacgcgg gcgacgacgg cggcggcggc cggctcgtcc ttgtaccggg ccaggtaaag
    11881 gtccagctca agcccccggt cgcgggcttc ccggaagaat ttccggaaca gggattccgg
    11941 atgccggggc agcccctttc gcgaggccgt ccggccgtag agctcatgga aggttcggag
    12001 tccggcttct cccgttcgtt cgacgacggt ccccctgcgc tccgcgagcc gcacggcgta
    12061 gcgggtcttc gcctccatcc tggaccagac ggccgccggg ccgcccgtca gatccaggat
    12121 catcgtatcg gggcaggtgt attcgaccgg ggccttgcgg aacctgcgcc gcacggtcga
    12181 cgcgttcatg cgcagctcct gcagtccccg gttcaatgct gatccgcggc cgtcggtcca
    12241 ggcctctacc atgaggtccc agcggatgaa gacgcaatcc ggggggagct tcgggagcgc
    12301 gcgaagggac agggcctcca tcgcggcgcc ccgctcgtcg atgtcgcgcg cccggaggcc
    12361 cggaagggac agggggccgg ggatgtaggc catggagccg gccgtaccga tccgcctgca
    12421 aagggcggaa cagcccagcc ccgtctccgc ccgcttccag ccctgcgatt ccttccaggc
    12481 tatccaggcg ggatgccgaa acagggaaac gggcgcgggg tcagtcatcc tggaccacct
    12541 tctcgacgag gacgcgccgg cggcctccgg gactgtcgta gccgaaggat tcacccgccc
    12601 gccggcccag gagttcggac ccgagggggg tcaggaccga gatcctgctg gtggagatgt
    12661 cggcttccgc gggaaatgcc aggcgatagg agaaaacttc cccggagtcc tcgtcccgga
    12721 tcgtgatccg ggaattcatg gtcacctgga tgcccgccga atccgcggat cccgcacccc
    12781 gggctatccg cagacgttga tcgaggacgg ccgcgcggcc gggatttcgg ctgtttcgca
    12841 tcgccagggc cagggcggac agcttccggt agtccgaatc cgtgagtgcg tagagttcca
    12901 tcctccgctc cttgtgagta ggtatgtcgt ttgagaataa gaacatacct gctcgggacg
    12961 aaattgggaa ctctcacatg tcttgtacgg aatttgtatg aatatctaca agtaaggatc
    13021 gaatccatcc gcggcgcttg cggacatgcc gcccaggagc cccgcaaggt tcacggcccg
    13081 gtcggagatc ctgagtttcc ggcgcaggtt gtggcggtgc cgctccacgg tagcccggga
    13141 gatccccaac agctcggcga tctccttgct ggtccggccg ttcctcacct gcacggcgat
    13201 ctccctctcc cgggggctca gggccgcgcg gccctcgggg ccccggccga tggagtcgac
    13261 atcccgttcg agctcccgga tcaggagctc caggtaggaa tcccggcgtt cctccgtcag
    13321 ggagcgatcc cgggcgcgct cggcgagggg caggatctcc gcagcggcgc gttcccggaa
    13381 ggccagcagc gtcctgcggc gatcggcctc gatcgcggag agcacctccc ggagggccac
    13441 gttcttggcc gaaagatcct ccgaggccgc tcgaagccgg gtgatcaggt tcgaggtccg
    13501 gaggatggac gcggcgatca cgaggatgga atccaggagg gccttttcct ggggcaggaa
    13561 ggacaggtcc ggatccaggt actccatgcg gacgcttccg ctccatccga cggagtcctc
    13621 gtccggcagg ggggcttcca gcgcgggtcg gagggcgccg tcctcgggga cgggggggcc
    13681 cctgcgcacg gtgacggtat cctcggagcc gatcctccgg aagcgtacga cgcaggccgc
    13741 ccgggactcg tgctggacgg ccgcgcacaa ggcacgggcg attccctcgg cggcgtcccg
    13801 gggctccggg ccgccggcgg ccaggaggca gatggaatag acgcaggcca gctccttctc
    13861 ccgttcccgg agccgttctt tcagggtagc catggctcca tgatatccca tgcgggaaac
    13921 ccgtcaacgg cgctaggatg ggcggcctcg gatggagtac actcgccggg gaaaccgcca
    13981 tggaaaccat ccggaccgtg ttcaagatcg gctacggccc ctcaagcagc cacaccatgg
    14041 gacccgcccg ggcggcggag tccttcaagg aacgcgcgcc cgagggacgg tacgaggtga
    14101 ccctctacgg gtccctcgcg gccacgggga aggggcacct cacggacgag gcgatactca
    14161 aggtcctggg cccggaggtc cggttcgagt ggaagcccga taccatcctg cccggacacc
    14221 ccaacgccct cacactcgag gcccgggacg aggccggtcg gtccgtcctc tcggagacct
    14281 ggttcagcgt ggggggaggg gaggtcaccc ggaccccggg cgtccgttcc gcggaatccc
    14341 ggtacgacct cggccgcatg gacgggatcc tggcctggtg ccgggaggag ggacggaacc
    14401 tctgggaata cgtcgcggag cgtgaggacc ccggcatctg ggactacctg ggcggggtat
    14461 gggaggccat gggagactcg atccggaacg gactggacca cgagagccgc ctgcccggcc
    14521 ctctgggggt ggcccgcaag gcctcctcgt acttcacaaa gtcccagctc cagggcgacc
    14581 cgatccgggt ccggacccgg ctcttcgcct acgcccttgc cgtggccgag gagaacgcct
    14641 cgggcgggcg ggtcgtcacg gcccccacct gcggatcctg cggggttctg ccggcggtgc
    14701 tgtatctcct ggaggaatac tacaagttca gcgagaagcg cctgctccac gccctggcca
    14761 ccgcggggct ggtgggaaac ctcatccggc acaacgcctc catctccggg gcggaggtcg
    14821 gctgccaggg ggagatcggc tcggcctgcg ccatggccgc ggccgccgcc gcccagctct
    14881 tcggagggac ccccgcccag gtggagtacg ccgccgagat gggcctggag caccacctgg
    14941 gcctgacctg cgatcccatc ggggggctgg tgcagatccc ctgcatcgag cggaacgcct
    15001 tcgcggccgc tcgggccctg gacgcggcgg agtacgccct cctgtccgac gggagccatc
    15061 gggtgagctt cgaccgcgtc gtggaagtca tgcgtcggac gggccacgac ctgccgcacc
    15121 tgtaccggga gaccgccctg gggggattgg cgggggacgg tggacggtaa acactaagcg
    15181 gtgaaggatg ccggtctcat agcttgttca cctgttcacc tattcaccga tccaccttct
    15241 ttcacacctc ctccgcctcc tcgacctcct ccgcgcccgg ggaaccggcg cccgccgcgg
    15301 gaaggcccgg ggcggagacg gcacccaggg agaggctccg gaggatctcc tccacctgtc
    15361 cggccgcccg ctcccggtcg tcgatggagc atccgtggcg gaacagcccc ccgtcctcct
    15421 cgacccggcg gaccgtaacg gacaaaggaa ccctccgttc cccctccgcc gtgatgggca
    15481 gggccaggct cagggcgagg cgcgtgccgg gctcgatccg cccgggcgtc accaggagga
    15541 ggcccgaggg cgacaggtcc ccgaggtggc cgagggatgc gccttcggga tcgaagatct
    15601 ccaggttata gaggagctcc tccctccgct ccctgcgtcc gagccggccc tgggccaggt
    15661 ccccgtccgc ccggtaccgg cgcaggaccc ggttgcggtc caggcccccg ttcttgaggt
    15721 agcccgccag ctgggccagg acccgggcgg accgctccgc gtcgtgcacg gaggtctcgg
    15781 attcccgggc cgcctccagg atgccctcgt aggtctcggt acgctcccgg aaggcgacgg
    15841 cgaagccctc caggagggag gcggcctggc ccgcggaggc ggaggagcgg ttcgagccct
    15901 ccgcctgctc ggagaagacc tcgtcgaacc gagccaggcg gccctcgacc tcccggatcc
    15961 ggccgtcgat ggcctcgaag gaggaccggg tgcgcgagag cgagcggtcg agctgttccg
    16021 aatgggaggc gacctgggcg gacagggccc cggattcccg gatccccgcc gccacggagc
    16081 ccaggacctt gccgatgtcc tccagggcct tccggctggc ctcggagagg ttccgaacct
    16141 ccgcggcgat caccgcgaag cccgaacccc gggcgccggc ccgggccgcc tcgatggagg
    16201 cgttcgtggc caggatgtgg gtgcgctcca cgatgtccgc gatccgggcc aggctttcgg
    16261 cgatgcggtc cgcctcctcg gcctcccggc gggccgcttg gttcaggcgg tccaggaggc
    16321 gttccagccc cgaggcggcc tcgccggtcg cctcgactcc ctcgacgccc gcccgggcat
    16381 cccgggcggc ctcccggctc aggtccacca gcagcgaaag ctccgaccgc gaatccgagg
    16441 cggtgcgggc cagccgggcc gcaagttccg cgctcccggt cagccgttcc cgcaattgtc
    16501 cgagctcctc gttggtccgg gcctgctcct cgaagaactc cgtgacctgg cccctcagcc
    16561 gggtcagcat ctccaggtcc cgacgggcca gcccggccag ggtctgggcc gagaaaagga
    16621 tatccgagga gaagaggtcg aatttcacgg ccgagcgctg aaggagggcg atgtcctggt
    16681 tcaaggtttc caggagccgg gcgtgccaag ccagggaccg atcctccgag gcggccgccg
    16741 ccaggcggac caggtcgagg gctccccgct ctgggtccga ggacgcggcc tggagggcgg
    16801 cgtccccccg ggaagctttc cgaacggata cgtaggctcg gagaccgacg gccgccgcga
    16861 gcagggcgag ggccgccgag gcggccgccg cgggacggcc caggccggga accgccaggc
    16921 ccgtcgcggc ggatcccgcc gccaggatcg cggtcagggc ggccgccagg accggccgct
    16981 ccgtccgatt cgcgttccga tgctttttca tggccttcaa gtctagcgct ttttgggaat
    17041 aatgcacgcc ggaaggttca gcttcccggc acgctccccg cgggcggggc cttcgggggg
    17101 tccgcgtact ccacctggac ctccgtcagc cacttgaccc acttcgaccc ggtcagggag
    17161 ggaaagaccg cccgcacggg gaacccgtgt agcacgggta ccggtcgccc ctcggcgaac
    17221 tcgtaggcca ggaagttgtg gggggcccgg gcgtcctcca tgccgatcca ggtcgagtat
    17281 ccgtcggcgc tgaccaggtt cacgcccttg gccccctcca ggggctccgc gagttccaga
    17341 agggccgcca gggaggcgcc cgcccagacg gcgttgtcct cgaagtagcc cgggcagacc
    17401 agggtggacc gggagcgcac ccggggcagg gttcgaagct cgtcgaagtc gagggaaagg
    17461 ggccttcgca ccgctcccgt gaccgccagg cgccaggtct tcaggtccag ctccaggggg
    17521 gtgcccgtca tgtggaggcc cgagtcggga tcgagctcgg cgtagccggg aacgaaggcc
    17581 ggcatcttgg gcgggaccac cggcgggagt tcggaccggg aacgggccgg gaggtactcg
    17641 ggatccgcgg acgcgggaac cggagcctgc gaagggggcg ccccggaagg ggaggtcgtc
    17701 ccggggccgc aggacgccat gccgagaacc aggaccgccg cgaggaaggc ggccgggatc
    17761 gatgcgccga ggtcggcccc gcgtgccggc ctcacggttt cctcccggcc gcccgctccg
    17821 gggcgggcac ggaaaggagg agccccaggg cgaagcgcag ggattcctcg tcccagggtt
    17881 cctccgcggc gggaagggcg aagaaggccc aggtcctgcc ggcggtccgc ccgaggggga
    17941 cgaagtcccc caggggtatc ccggaatccc gggcggaccg gatgaccccg ggatcggact
    18001 tgatgccgac cccgccttcc atgaggcaca gccgcattcg gccgtactcg aaccaggccg
    18061 ggtatccgaa catcttccgc ttcaggaccg ggaggatctg ggggagggtg gcgtccagcc
    18121 atcgttcgca ggacgggttc caggaagcca tggcatctcc tcggatgacg ggcaggtatc
    18181 cccgggaatc ttctacataa gatactcacg cggtagtcta atcaaaaagt cagaaataag
    18241 ggtcgcccgc ctcgccgggg tggtggccgc cgcgcccgcg tcgggtatac tggagggggt
    18301 ggctggcagg gtgctgacga actcggagta tgtcagtatc cggccggatc accgccaagg
    18361 agaaggccgt gaaactgaaa ccctggattc cccgggagtc ggtgaagatc gaactgggcc
    18421 ggcagagccg ggtgttctta agtgtgaccg ccctgttggg gcccgcctat gcccggttcg
    18481 tcctgcgcct ggctcggatc ggcgtggagg ggcgggagcg cctggtcgag gagtaccgta
    18541 ggtccgccgc ggacgagggg cgcttcatcc tggcctaccg ccatccgggg gacgcggatc
    18601 cccacctggt cttccacgtc ctgacgaacc tgatccgggg caaggcaccc gccgtggagg
    18661 cggacggccg gcccggggcc tggtatccct cgggctcgga ggtccaactc tgggccagcc
    18721 ccctggtgca ctgggcgctc cggaacgcgg gcatcgtgcc ggtgcgccac ggcgccgtgg
    18781 accgggcggt cctggactac ctggtgcgag ccgtggccga gcggcgccgt cccatggcca
    18841 tagccccgga gggcatggcc accctgaacg gggggatcct cccggaactg gatcccggaa
    18901 ccacccggat cgccctcctg gccgcggagc gtctggcctc ctcggacaag cccatgccgg
    18961 tacggatcat ccccctggcc atcgagtatt cctacggtcg gaccaccagc cccgcccgcc
    19021 tgggccgctt cctgacccgc ctggagaaac ggatcggcct ggaccgggcc gcagctcgcc
    19081 ccgatcccgg gcagggcgct accgggaagg atccgacctc gccggaggcg atcaagtccc
    19141 ggctcctggc catttggggc aggctcgtgg atatcgcgga gacgacctat gcccgttcct
    19201 ggggcttaaa gccggccccg acgggcgcgg gcctggcgga acggctgggc gtcctgctgg
    19261 acgcctcgat ctcccggatg gaggccttct acggggtgca gcccgcggcc accctgaagg
    19321 cccggatcct gaacatccgc gcggagtccc tgcgccgggt cttctattcc cgggaggagt
    19381 tggcggccct gagccccctg gaactcgcgg ccgcgcggcg gggctccgcg gaggcctatt
    19441 tcctggacaa gatctacatg accgctgccc tggggcagtt tctggatccc gggtacctgg
    19501 acgggaatcc gagcttcgag cgcttggtgg agacggccca gaacctccac gacttcgcca
    19561 accggctgga gggcttggag ctcaagcacc gctccaagta cttcctgaag gacgcccggg
    19621 tcatcgcggg ggagcccgtc gaggcctcgc gccgccccgg ggaggggagg aaggaggcgg
    19681 cggaacgggt ggagcgggaa ctggaggaag gcttccggcg gctgatacgg cattagccgt
    19741 tcacgtgccc ggatcccact ccagagtccg tgacgcgaac agcgcttcca tcgagctcag
    19801 gtacgcttcc ttcgtgaact cgggcttgaa gctccgccgg atctccaggg ccttcgcgcc
    19861 tccgttcaag gccaggtcga aggcggcccg ggccaggatc cgggcgggct cccgataggc
    19921 ccggaccggg tcggagggct tgaagtccgc ggcgtggatg gtccccgaga agcccgcgta
    19981 cccgagctgg caggtgggca ccagggccgc cacgtccccg atgtcgtcgc tggcgaagga
    20041 ccggtcatgg aagtcgatgt cccgttccga aaggacggtc cgggccgcgg ccccgaggac
    20101 ctcccccagg gcgggatcgg gccggaaggg catgtagccc ggggagttgt cgatccggac
    20161 ggccgccccg atggcgacgg ccccggcggc cagggcccgg tccaccttgg cgtccgcgtc
    20221 ccggatggcg ggccaggagg ccccgcggac gtaggtctcc aggaccgcgg tgtccgggac
    20281 cgtgttcacc gtggtccccc cggacttcat caccgggtgt acccggatgt ggtcgtcgtc
    20341 ccggaaggtc tcccgctggg cgtggatggc cagcatggcc agacttgcgg cgttcagggc
    20401 gttgatcccc ttctcgggcc aggcccccgc gtgggcgctc cggccgagga aggtggcccg
    20461 cttggccaaa aacccgttca gggtgccgtt caccgtggcg acccgctccg gagaatcgga
    20521 catggagtgg tacttgaggg cgacgcaggc gtcgtccagg gcgcccaggc ggatcatctc
    20581 ctgcttgccc gagaggaagc ggaccttccc ttccgcgcgg agacccaggc ggtactccat
    20641 ctccaggaac tcctccgcgg gggttcccag gaagaccagg cggaccttct cccgttccgg
    20701 aacccccgcc tccgccaggg cccggaagac ggccagcatg acggccatct gggcgtggtg
    20761 cccgcaggag tgggccacgc ctccggcggc cccggaggtg aggatcgcgt ccaggtccgc
    20821 gatcaggaag atcgccggag cgtcctcgga gcccaggtcg gcccggattc ccgtgagggc
    20881 gatgcgggtc cgcacccggg ctccggtttc ccggagcgcc ttttccagga cggcggcggt
    20941 gcgctcctcc cggaacccga gctccgggtg ggcggccagg tccgccgcca tgccctccag
    21001 gtccgccgcg ccccggtcca gggccgcctc gaccgcgccg aaaccggtat ctcccgccat
    21061 ccccgcctcc ttcacaagct tcgtgacact accctagttc cgccgggaat ctttttcaac
    21121 cggccgaggg cgccgcggag ttttctcgac cgccgtcacc gcccggcccg cgcctcgggc
    21181 ttggaatcct gactaaacgc tattgatttg ccccggggcc ttggaatatt cttaatgctt
    21241 ccggagaacc gcggagccgc ggcccccgcc gcgcccgatg tatcgcgagg aggtttccat
    21301 gaagcgtctc acgctgcccg tcgtcctgat cctggccctg gccttcgccc ttccggcggc
    21361 ggcacagacc acgaacgtgt tcctgctgat ttccggatcc ctgggcgaca agggattcaa
    21421 cgactccgcc aaggcgggcc tggacatgat caaggccaag tacggggcca aggtcaccac
    21481 gaagtacacc gagctgggcg cggacacggc caagtacgcc ccctccctgg aggacgccgg
    21541 agccgagggc tacgcggtgg tcttctgctc caacaacttc cgaagcgccg tccaggagat
    21601 cgccccccgc tatccccgca ccctgtacgt catgtacgac ggctgggtcg agggcgacat
    21661 ccccaacgtc tactcggtgc agtacaagaa ctccgaggcc ggctacctgg cgggcgtcct
    21721 ggcgggaagc atgacccgga ccggcggcaa tcccaagatc aatccggaca acgtgatcgg
    21781 tttcatcggc ggccgggaca tcacggggat caacgatttc ttcgtcggat acatcgccgg
    21841 agcccagaag gtcaacccgg cggtcaagat cgtctacaac tacgtgaact ccttcacgga
    21901 taccgccaag gccaaggacc aggccctgat ccagttcggg tccttcaagg ccgacgtggt
    21961 ctaccacgcc gcgggccgcg cgggcctggg gctcttcgac gccgccgtgg agaccggcaa
    22021 gtacgccatc ggcgtggaca cggaccaggc ctccctcttc cccagcgagc cggccaaggc
    22081 gggcctgatc ctctcctcca cgatgaagcg cgtggacctg accctggcgg gaaccttcga
    22141 caagttcatg gccggccaga agctcggggg ccagaaggtc tacctgggcg tggcggacgg
    22201 ggtcatcggt ttcgcccccc tgaacaaggc ggtgccggga gccatccaga aggagatgga
    22261 ccggatcatc gccgagatca acgcgggcaa ggtggccatc ccctcggcct tcagcatgag
    22321 cgccgaggag atcaacaaga tgcgggacgc cgtcaacccg aacaagtaag atcgttccgg
    22381 aggctgtcca aaaagctcag ttggcttttt ggacagctgg ttctagggaa gcagttccta
    22441 acgttcctta aagatcgtta gaaactgcaa ggggtgtacg ataagtaacc gacttattgg
    22501 acaccccgtt tatcttttct agccgccggg accgccgcgg gcgcaccgca ccccccggcc
    22561 cggcaaggaa gcacgcgagt ggaaaccatt ctccaggccg tggacctctg caaggtgtac
    22621 ggcaacggca cggcggccaa caaattcgtc aacctgaccg tgcgccgggg ggagatcctc
    22681 ggcctggtcg gggagaacgg tgccggaaag tccaccctga tgaaggtggt ctacggggag
    22741 gaacctccca gctccggaac cctcctcctg gacggcaagt ccgtccgctt cgggtcctcc
    22801 cgggacgcca tcgccgcggg tatcggcatg gtccaccagc acttcatgct ggtgccctcc
    22861 ctgaccctgg cggaaaacct cgtgctcggc cgggaacccc ggaagaagtc cgggatgtac
    22921 gacctgaacc gggcggtccg tatcgccgag gagctctcgg ccaagtacaa cctgcgcatc
    22981 atcccccggg cccgggtggt ggacgtctcc gtctcgatgc ggcagaagct ggagatcctg
    23041 aagaccctgt accggggggc ccgcatcctg atcctggacg agcccacctc ggtcttgacc
    23101 ccccaggaga cggacgagct cttcgcggag ctcaaggccc tccgggacaa gggacagacc
    23161 atcatcttca tcagccacaa gctgggggag gtcttccagc tctgcgaccg ggtgacggtg
    23221 atgcgggacg gggcggtcat cgccacccac gacatcgggg acgtggaccg ccagactatc
    23281 tccaacgaga tggtggggcg ggaggtccgg ctgaccctgg acaagaaggc ggccgccccc
    23341 gggggaacgg ccctggaggt ccgggacctc tccttcgtgg acgacttcgg gatcaccatg
    23401 gtggaccggg tctccttctc cctgcgcaag ggggagatcc tgggggtcgt gggggtggac
    23461 gggaacggcc agtccgagct ggtccgcatc ctgaccggcc atgcccgcac cctgaccggg
    23521 tcggtgcgca agggggggac ggacctgacc aacgcatccc caaggaaggt ccgcaaggcc
    23581 gggatggccc acgtggccga ggaccggatg accgtgggga ccgccctgcc ggccagcatc
    23641 gcggacaacc tcctggcgga ccgctactcc gagcccgcct tcaccggagg cggcttcttg
    23701 cggagatccg ccatccaggc ggaggccaag ggcatcgtgc agcgcttcga catccgttgc
    23761 tcctccccga accaggcggt ggccagcctc tcggggggga acatgcagaa ggtggtcatc
    23821 ggacgggagt tcaccgcagc ggcggacgtc ctggtggtct cccagcccac ccggggggtg
    23881 gacatcgggg ccatcgagtt catccatagg accatcctgt ccatgcggga cgagggcaag
    23941 gccatcctcc ttgtgtcctc ggacctgggg gaggtcctct ccctctcgga cagcctcatc
    24001 gtgatgcact ccgggaagat cgtggcgtgg ttccccgacg cctccgccgt gacggacaag
    24061 gagctggggc tctacatgct cggcctcaag acccaggacg aaatcgagat caggaaggtg
    24121 ctccatgagt gacggaaccc gaaccaagac tcttcccggc aaggtcgggc tcttcttcac
    24181 gaaccgggac gccctggagc ccgccctggt caccctgttc gccgcggtct tctccctcct
    24241 ggcgggggtg gggctcatat tcctggtcag caaggagccc gcccagtcgg cgcggaactt
    24301 cctgctgggg cccttcctgc ggacctacaa catctactac atgatgatcg ccatggtgcc
    24361 cctgaccttc acgggcctgg ccctgtgcat catctaccag tccaagcaca tgtccctgat
    24421 cgtggactct tcgttctaca tcggggcggt ggtggccacc gcggcgggca tccacgtcgc
    24481 cctcccggcg ggctggcatc ccgccttcgc catgctggcc gcggggatca cggggggctt
    24541 cataggcctt ctgccggcga tcctcaagat ccggtggcgg gccaacgagc tcgtctcctc
    24601 cctgatgctg aactacgtct tctacttcgt cgggagcttc gtgatcctct acttcctccg
    24661 ggaccggaac cagaccatcc tctcgtccct gccgttccgg aataccttcc tgctgccccg
    24721 gctgattccg aatacccagc tccacgcggg attcatcgtg gccgcggtct tcgtggtcct
    24781 ggtgtccgtc ttcctctatc ggaccaagtg gggctacgag atccggatga tcggggccaa
    24841 ccagaagttc gccgcctacg cggggctttc ggtgccgacg gtcatcctct attcccactt
    24901 cgtgtccggt ttcctgggag gaatgggcgg ggccatcgag atggcgggac tctacaaggc
    24961 cttcatctgg cagatgaacc cctcctacgc ctgggacggg gtcatcgtgg ccatgctggc
    25021 ccggaagaac cccaagttcg tgcccctctc cgccttcttc ctggcctaca tgcgggtggg
    25081 ggcggacttc atgtcccggc ggggggacgt ggccttcgag ttcatcaccc tgctccaggg
    25141 cctcatcatc ctgatcctgg cctcggacaa gatcgtgaac tacttcaagg cccggaggct
    25201 ccagcggacc gccctggcgc ggaccgccga ggcccgggcg gccggggctc cggcgggagg
    25261 cgcggaatga tggaattcct ggcctccgta ttcggctcct cgaactactg gttcatcgta
    25321 gtgcggtcca cggcccccat actcctggtt accctggggg cggtcatcac cgaacaggcg
    25381 gggatcaaga acatggccct ggagggctcc atgctctggg ccgccctgac gggggtcctg
    25441 gccagcgcct ggaccgggag cccctggatc ggcctggccg cgggcgtcgc cgcgggggtg
    25501 gccatcgccc tcgtcctggg gctcttcgcc ctcaagctgg acgccaacgc ggtcctggtg
    25561 ggcatcgccc tgaacctcct ttccacgggg gggacggtct tcgtcctctt ctccatcacc
    25621 ggggacaagg gcatctcctc ctccctgccc agcaaggtcc tgcccatggt ctcggtcccc
    25681 ctgctgcggg acataccctg gttggggaag gtcctgtcgg ggcagaacct gatcgtgtac
    25741 ctggccgccg ccgcggtggt cgtgatccac gtgatcctga acaagctgac cataggccgg
    25801 cggatccgtg ccaccgggga aaacgtcgag gccgcgcggg cggcggggat cgacccggac
    25861 cggatcaagt tcttcacgct gatcgtctcc ggagtgctgg cggggcttgc gggctgcttc
    25921 ctgtccatgg gctacacctc gaacttcacc tccgggatct ccgcgggccg gggctacgtg
    25981 gccaacgcgg cccaggtcgt tgccggcggg accgcggtgg gctccatctt cgcggccctg
    26041 atcttcgggg tgaccgacgc cctggcaacc ttcctccaga gccggggact cccgcccgag
    26101 ctgatccagg ccctgcccta cgcggtgacc atcctgggct tcgtagtggt gaccctggtc
    26161 cgggacaggc gggaggccgc ccggaagctc cggctcttcg cgggcaggga cgaccggtga
    26221 cctgcgacct cctgatcgag aacgccgcgg tcctgaccgt ggacgcggcg tggaacctgt
    26281 acgagccggg cttcctggcg gtgaaggggg actcgatcgc ggccgtgggc cccatggcgg
    26341 tgcgccgggc ctgggaagcc cggaccgtcg tggacgccgc ggggaagctc ctcatgccgg
    26401 gcttcgtgaa cgcccacacc cacgtggcca tggccgcctt ccggggcgcc tgcgaggacg
    26461 tggacgaccg cctgcggaag tacatcttcc ccctggaggc ccgcctggtg gaccgggacc
    26521 tggtgtaccg ggcctcccgg ttctgcctgg tggagatggc cgcctcgggg accacctgtt
    26581 tcggggacat gtactacttc gaggacgagg tggcccgggc cgcccgggaa gcggggcttc
    26641 gctgtgtcct gggggagacc gtggtggatt tcccggcccc ggactgcccg gagccctacg
    26701 ggggtctcgg gtacgggctc gacttcatct ccgactgggc cggggacgcc ctggtgaaac
    26761 cctgcctggc cccccacgcc ccctataccg tggatgccga gcatcttcga agggtccgcc
    26821 gggaatccga ggcccgaggc ctcccggtcc tcatgcacat agcggagacg gccccggaga
    26881 acgagcgctt cacggcctcc cacggctcgg tgctgcgcta cctggacgcc gagggagccc
    26941 tctaccccgg cctggtcgga gcccacatga tctacctgga cccctccgac atcgccctgg
    27001 ccgccgcccg ggggctttcg gtggcccact gcccgggctc caacgccaag agcggccggc
    27061 cgatctgccc cgccgcggat cttgcggacg cgggggtgcc ggtgggactg ggaaccgacg
    27121 gccccctgtc cggaaacggc atggatctgc aggggatcgt cggacttttc cccaagctcc
    27181 agaagatgcg ccggaacgac cgcggggccg tccccgcccg gcaggcggtc cgggccgcga
    27241 cccggggggg cgccgaggcc ctgggcctgg gaaaggtcac gggctccctg gaacccggca
    27301 agaaggcgga cctggtcctg gtggacgccg attccttcgg cctgcagccc gtctacgact
    27361 ggtacgcggc ggtcgtctac tcgatgcgtc cctccgacgt cgccgccacc tacgtgggcg
    27421 gccgcctggt ctgggatcgc acgggcatga agggcttcga ccaggccgag gcgatggcgg
    27481 acgtccgcgg cgtcgcggac cggtgccggg gagtgatcgc ggaattgacg ggaggcgcgt
    27541 cgtgacggaa tcgaccccgc acatcgtacc cgaggggccc atcgccccct cggtcctcat
    27601 gcccggggac cccctgcggg ccaagtacat cgccgagcgt ttcctggaga aggccgttcg
    27661 cttcaactcc gtccggggga tgctcggatt cacgggcacc tggcgggggg tacaggtttc
    27721 ggccatgggc tccgggatgg ggatcccgtc catggggatc tactcctacg agctcttctc
    27781 ggcgttcggg gtccgggaca tcctgcgggt cggcacctgc ggaggcttcc gggaggacat
    27841 ggagctgggg gacctcgtct tcgcccaggc ttcctccacc tcgtcctcct gggcggagca
    27901 gtacggcctg ggcggaacct tctcggcgat cccggacttc gggctcctgg aggccggagt
    27961 ggccgccgcc cgggacctgg ggaggcgctt cgtggtgggg aacacggtct gcctggacca
    28021 gttctcccgg taccacgccg tggacaagcc ctggaccccc tgggcacgga tgggctgcgt
    28081 ggccacggac atggaggcct acgccctgta ctgcaacgcc gcctacctgg gccgccgggc
    28141 cctggcgatg ttcgcagtct cggacctccg cctcaagggg ctccacatgg aaagcgccga
    28201 ccgggagcga tccctggact ccatgatcga gacggccctg gagacggtcc gcagggtccg
    28261 cgccgcggag gcctgacggc cccgcgtccc ctacagatcc atggtggacc ggtagggccg
    28321 ggtcttccgg cggtgctccg cccgggccgc cgcgaggagg gccgggtccg aggccagggc
    28381 gaagcccgtc gcggccagga ccgccgccgc ccagcgcatc cgcttgaacg cccccgttga
    28441 gcccgaggcc gagcaggact gccaggtgtg ggctcccacg cccaggggcc aggtggccgc
    28501 gttcatctgc cccgtggggg tcacgtagga cacgtccccg gtgtccaggg atcccccgat
    28561 ccggaaccct tccccgaagt ccccggcttg gtcgtgtgtc ggccgcttga gcagggacag
    28621 gggcgccccg atcatcgtca gggtcgactc gcggtcccgc ttggggaccg tggccgccag
    28681 gtcccgggcg aaggcggtct cctggggggc cagggcgggc acggggaggc ggcgcatcgc
    28741 ccccagcatc gccccgtgga tggcgtcgtt cgggatgtag tcgcacttcc cgctgctgac
    28801 ctcccaggag acccgggtct ccgtcatcat cgcggccccc ttggccgcct tgagcacccg
    28861 ggcgacggcc aggcgcaggg cttgggccgt gggggcccgg acgtagaggg taaccgcggc
    28921 gctctcgggc acgatgttcg gccgggctcc cccgtcggag atgacgtagt ggatcaggac
    28981 cccccgctcc acgtgctccc ggaggaactc gaggcccagg ttgaagatct gggccccgtc
    29041 cagggcggag cgtcccgcgt ggggggccat ggccgcgtgg ctggcccggc cggcgaagcg
    29101 gacgaccagg gccacgttgg cgctggtggt gtagcggtgc ggggtattca cgtccgcggg
    29161 gtgccaggtc agggccgcgt cggcgtcgtc gaaacgcccc gccttgacct gggggatccg
    29221 cccaagggcc tcctcggcgg ggcaaccgta gtaccgcacc gtgcccggaa gacccgcggc
    29281 cgcgagggcg tccgccaggg cctccgcggc ggcgagggac ccgatcccca ggaggttgtg
    29341 cccgcaggca tggcccgggg cgcccggttt ccgggcctcc cggcgtaccg agcaggcctg
    29401 ggacaggccc ggaagggcgt cgtactcccc catgagggcg atcacgggct tccccgcgcc
    29461 ccgttcggcc gcgaaggcat ggggcatctc gggatagatc gtgatgcgga atcccgaggc
    29521 ggacaggcgg tcccggtgca gggccgcgga ccgctcctcc tcacccgagc gctcggggtg
    29581 ttcccagatg tcccgtgcca ggtcgtcgta ctcacccgcc cggccctcga tgtagttcca
    29641 tatatggtcg gtcatggccg cccctggggc acccgctcca gccgggagac atcgaagccg
    29701 agcctccggg actcctccac caggccctcg tagacctccg ggggagggac ggggtccttg
    29761 gccatgagcc agaggaagtc catcgtgccg ctggtcacca gcatggtccg gtccgcctcg
    29821 ttccagtaga ccaggcggta gggcagccag gtgaagggga agggccgggc ggacatctcc
    29881 ccggccaccg cgggatccgg gatccggagt ttctgcctca tcaccccctt cttgccgtcg
    29941 aagccgccct tgtacccctc gtagaggacc cgccaggtcc cgtcgtcgtt ggggatgtag
    30001 gtgtccgtgg tgcccaccca gtcccgggcg acgggaacgg ggatgcgggc gatctcgtac
    30061 caggccccct cgaagaggcc gaagtcgatg gaccggaccg cggggatgcc cttcgtggag
    30121 ggggcgcccg agcaggacgt caggactgcc agggcagccg ccgcgagggc cgcggcggta
    30181 cgggccgaga gtcggatccg ggtcatttcg aagtcccctt ctccgcgggc cggggcggta
    30241 gggggaggaa gatgctggtc ctgcgcttgt actcctgata agccgggtcg gagccgtact
    30301 tggcgtccgc gctcttctcc agaagtggga taccgctgac gaagaggagg aggaccgtga
    30361 tgaacaccgg ccccaggacg gagatccaca ggaaacccga gagggccgga accgcgtaca
    30421 ggaagaggcc ccaccagagc agggactccc cgaagtagtt gggatgccgg gaccaggacc
    30481 agagtcccgt ggtgatcacc cccgatcttc cggccttctt gaacgcggat ttctgcgcat
    30541 ccgcctccac ttcgatcacg aaaccgacga gccaaaggac cattcccacg atctcgaagg
    30601 tgccgaagga ccgcggcgag acggaggagg cgatggaagc gacgacgggc agcaggatca
    30661 cgaacacggc caccgcctgg aggctccaga agctggcgaa cttccacgga tcgtcccgct
    30721 tgtcgtcgaa gcggtggtcc accttgatct tgatgatccg ggtgaacagg tagcttccaa
    30781 gccgggccgc ccagagcagg gtgaggaccg cggggaccag ggccgccggg gaggccccga
    30841 ggttccggag tatgaggacg gcggacacga gggcgaagga aaggctatag gacaggtccg
    30901 tcaccacgtc ggtcttgcgc agggccgcgt aggcgaagaa gatcccgttg atcgcgacgg
    30961 ccgcgacggc ggaaaggacg aaagggttca agatgcctcc ttttcgatat gcattcctaa
    31021 aatcctatat tcccgggcga aaggcaacga cggacgaggg ccttcaggcc gccttgcaca
    31081 cgtaccaggc cgcccggggt ccgacccgga ctacctgcac ggaaggaaag gcttcccgca
    31141 gctccggaag ccccgtccgg atgaagttga agtagtcccc gtgttccagg tattccacga
    31201 tcgtcaccag gaggctgcgg tccggggtgt agtcctggaa gaggaccgtg tcccgggaca
    31261 ggcgccgcat ctccgcgaag agcttggccc ggtcctcccg cctaaggccg tgggccacgt
    31321 aggccgcggt caccaggtcg aagctcccgt ccggctccgc caggccccgg agtgcgtccc
    31381 cggggtcgca ggaaagcccc cggctccggg cccgctccag catggcccgg gcaacgtcga
    31441 ctccccggac ctcccagccc tcccgggcca gggcccgggt gaaggccccg gtgccgcagc
    31501 cgatgtccag ggcccggcgt cccgcgggat ccgggaggaa ggagcggccg agctcgaagg
    31561 cccgggcgta ggtccggagc tggccgtgga agaaccaggc gtagggccgg gcgatccgct
    31621 cgaagaggcg ggcgtggcgc tccgcgtggg agaggccgga atcggggtgc atacctccag
    31681 ggtagcggat ccgccgcccg ggcgccagcc gccggggccc gacgccgacg gggtcgccgc
    31741 aaccggatac ggtggtacgg cgcccccggc ctccctacat cgcggaccga tgttgatccg
    31801 gcaccggctc gtcggccaac tccaaaaggg cctgggccac gtggtagatg tggcggttga
    31861 cccggtgcag gtggtttagg aggtccatgt ggatctccgt ggtctctatg gagacctgga
    31921 cgccctcgtt caggcggcgg aaatgcttgc ggcggaactc cctctggagt tcccagcagg
    31981 actcggagca ttccaggacc tgccgggccc ctcccgtgtc gttgtcctcg aaggcccgca
    32041 ttactaggcg gaaattgtcc gccacgacct tggccagctc ctgcacgtcc gcgagaccct
    32101 cgtcggagag ccgtaagtcg ttgttgatga gcttcttggc ccggtcgcgg atgttaccct
    32161 cgatcacgtc cgccaggttc tccagttcgc tgacgatgaa gaggtaggag acctggcgct
    32221 tgctctcgct ctcggtcagg gactgcttgg ccaccttggt caggtatcgg acgatctcgg
    32281 tgcgcaacgt gtcgatgtac ttgtcctcgg tcttgatggc cttgccgtcc tcgggacggc
    32341 gttccgagaa gagccggacg ctggcctcca gcatatgctc gatgatgggc cgcatccgga
    32401 ggatctcctt cttgacctgg aagagggcca ccgagggttc ggtcagcatc gcctcgctca
    32461 gatattgggg gccgaaaacc tccttgggag gctccggggg caccaggcgg tcgaaggcct
    32521 tgacgatgag gggcagtagg gggatcacga ggatgtgatt gaggacgggc atgagggtgt
    32581 gggccatggc gatctcgcgg gccagattct cccgtccccc cgtgaacagc agggacagtt
    32641 cccgggccag gaagaggtag agccgatccc cctgaattgg tatggacagg aggatgtagg
    32701 caaggatcac tccgatggtc tggaagacca cgtggatata ggcgcttcgc ttggcctccc
    32761 ggttcaggga caggctcccg aggacggcgg tgacgcaggt gccgatggag gctcccaggt
    32821 tgatcggcac cgcctgggcc aggctgatgg tcccggagat ggccatggcg atgactaggc
    32881 ccgaggtggc ccccgaggat tggacgatca tggtgaacac gagtcccacc aggattccga
    32941 tgaggggccg ctccaccgag gccatcaggt tcaggaaggc ggggagggta cggaggggct
    33001 ccatggaccg ggacatcatg tccatcccca ggaacaacag gccgaagccc aggacggtgt
    33061 tggccgcgga cttgtgctgt ttcttggagg ccaggaggaa ggcgaagaag cccagggcca
    33121 cgaaaagggc cgccaggcgg gtgatcttgg ggaaggcgaa gagctggccc aggaaggtgg
    33181 agccgagttc cgcccccagg gtgaccgcca ggctctgccg gaaggtcatg agccccgcac
    33241 ccaccaggac cgcctccagg accgtggtgg ccgaggaaga ctggttcacg atggtgaccc
    33301 ccaggcccgt gaggtagccc cggaccttgg tcttggtgag ttcgatcatg atggtccgga
    33361 gccgggcccc cgccagggcg gtgaggttgt tgttcatcat caccatgccg aagaggaaga
    33421 tcgccaggcc gccggccagg tcgaagatgt tgtagatgga catgccggat actcctcagc
    33481 ccttcccggg accacgcggg taccgggatt gtaggagagt atcgtcttaa ccgtattttc
    33541 gtcaaattct aaccgagcgg cccgccgagt ccccgccggt cgaatcgtgg cgggaccccc
    33601 cgtccttgac cggccggccc tgagccgcgt atcctcccgc catggatgac ctgctggagc
    33661 gggctctcga ggacggcccc ctgcttcggt cccgggtgga ggccttcctg ggcccggctc
    33721 cggaaaaggt tccggatttc tggaagcccg gcatcgggga gatccactac gttcccgtgc
    33781 ccggggcgga gatccggatc attcaggtac gaccggaccg gaccgcagcc cgccgccctg
    33841 tggtcctggt gccggggtgg ggtacgaccc ccgaaggctt ccgggaattc tacggccccc
    33901 tgcacgggaa ggcggagttc cttttcatcg agacccggga gaagaattcg agccggatac
    33961 agggccggaa gcccgacctg tccgtggaac gagcggcctt ggatatccgg gcggccctgg
    34021 aggcggcggg actcttggac cgggactacg tccttgccgg gacctgctgg ggctccgccc
    34081 agatcctgga gggcatgatc cggggatacc tggatccttc gaccgtcctc ctggccgacc
    34141 ctatgcacac cctgtggttc aacaagacct tcctgcgccg ggtgtccccc tggatacccg
    34201 ccgccgcgat ccgggcggtc cggcccctcc tgcggcaggc cctggtgggg gacctaaagg
    34261 aaggggctca gaaacagcgg accttcgagt tcatcgacag ggccgacgtg tggaagtgga
    34321 agaagtccgc cgaggcggcc tgggacttcg aactcttcgg ccgcctcggg gccgtgagac
    34381 gggaggtctt cgtcctcaac gggaccgggg acaaggtcca cgacccccgc aactaccccc
    34441 gcgtggccgc ggaactcccg gcgggccgct tcctccggat gcccctggac gaatccttgc
    34501 gggagcggat gttcggaacc gtcgccctgg agttcgcacg aggccgggcc ggggaaggac
    34561 tacccgctag gctggcggag ttcgaacggg cggtgcggta gggacggccg ccttgatcgt
    34621 cccccccccc tcagcgccgg acggggtcca gagccacgcc gcggggaccc tggagtccgg
    34681 acacgatggt ccgaacgtcc gagccgtcca gcttggcacg ttgaatcgtt tttgtcccga
    34741 gatctaccca gaaaaggaag ccttcatcag gatccacggc aagttgtcgg ggcggtgctc
    34801 ctgcccaaac gactatattc gtaacactgg ctgagctcag attcgcatca cagcggaaaa
    34861 tgcctccccc gggaccgtcg tcgctccaat acacatatcc gtgtaagtta tccacgatca
    34921 agtttccagg agttttaaat ccagcagata caggggttaa atccagtgtc agatccaaac
    34981 ttccattgga attaattcga tgtatctttt tattagccgg atcggctacg aaaaccttgt
    35041 ccagattcgg ataatacgcc agatcctgcg gatctccgat accggcgccc gccaacggac
    35101 tttctgcgga ggagtacggc tgtcgataga tcgtgtcatt ggagattccg gcccagaata
    35161 gaacttcctc ggcgatgttt ggactcaatc cccgtgggaa taccccccca atcggaccag
    35221 gttcctgata gagcacggaa aggccggttc cgtccaggtt cgaggagacg accttcccga
    35281 gattgctgat cgcggcgcat gtccagaaga gtttgcccgc gttcggatca actgcgatca
    35341 tggccaggtc gtcctggttg gaaagcggaa ggatctctat aagattcctt ccatcggttt
    35401 cgcagcggta gatgcgcttt gcatcccggt ccgtgatgta caggtgcttc gccccgccct
    35461 gggccagctt ttccgccagg tcccggaggg ccaggggctc gcaggtcgtc aggaccaggg
    35521 acagggcgag gcccgcggcg gccacgcggg ccgcacggaa taccttgttc attccgacca
    35581 tgtgtccacc ctccgggccc gctgggcctc cgcggcgcgg gcgttttcct ggcccgcctg
    35641 ggccaggtac tcgtagcggg acgccagggc gggatcgacc gccttgagct ggtcgaaggc
    35701 cctgcgggcg gtcccgtagt tctccagaag ctggctggcc cgggccagcc cgagcagggc
    35761 cgccgggttt ttaggtgccg aagcggcggc ccggtcgtag tgccggtagg cttcctcaac
    35821 ccggccccga aggagggcca ggttccccag attcaccagg gccggcgggt aagcctccgc
    35881 tttaatcgcg gcctggaagg ccttttcggc ctcctccagg aacccgtacc gggcgtgcag
    35941 gatccccagg gcgttgtacc ccttggggcc tccggagaga gccgccttct ccaggcccgc
    36001 gacctggggc cggtactcgc gttccaggag ccggcgccat acctccgagg ccgcggcccg
    36061 gaccttgtcc gccgacggag gctccgggac gggctccccc cgagcgaggc ccacgggctc
    36121 gtagagttcc caggcctggg ccacgggcca gagccgggcc ttgcccgcag cgccttgttc
    36181 ccgccactgc cgggctccca ggtgccaggc ctcgtcgaag cccaggtcca gggcggtcac
    36241 ctccacgggc acccaggctc ggcctgaggc ttcgaggaag tccgaagtcg agcggaaggt
    36301 ctttcgcatc cccgcggggg tggtgtccag ggccacggcc gtgtaaatgt gccccggcac
    36361 ggtgacgaag gctgtgccga ccccgacgga ttccaggagg gctgcgtata ggaccgtgag
    36421 gtcgtcgcag tccccggacc gaagccggag ggtctggcgg gggaactgca ggtagtccac
    36481 ggcgccggac ctagtggaga actccctgta gggggtggtc gggtccacgg agtagcggaa
    36541 cgaggcgagc cggaggatct cgaaggcagc aacggcagcg gctaggttcc tgtccacggc
    36601 agagcccggc gtctcggaag cctcggaagc cctccttgcc caggtcagta cgtcggggtc
    36661 cttgacggag acgaaggccg cagccttgcg atcgtcgtcc caaacggtcg cgttcctgtc
    36721 ttcaaaaccc aggctctcca cgtattcgtt ccggtaccgg attccaccgg aactgaattc
    36781 cacggcaagg actacggaag ccttggtacg ctcggatagt tccagtacta ggggattgaa
    36841 caaggccagc agggaatact ccgcggaggc tgccggcgct agggtccgcg ggccagaaca
    36901 tgtcttgggg tcatccatga actgccggga aaagaggctg accttgatat ccgtaatctc
    36961 ctgcttcccg gagttgacca gggtcgcggt cccgacggga ttctggtcgt agtgcttgta
    37021 gaacaccggg aacacgggat ccagccggat gtcccggaag cttaccgttg aaagaggctg
    37081 gagccgcatg ccctggggaa gcgcggaacc ccggcggtcc cgaagtccgt aggagacccc
    37141 gacgcccagg cccaggccgt gggacaggcc gaagcggccc cggtacccga gatccagaac
    37201 cgccgtatac cccttgggga aggtccagcc cagcctaagg cctccggcgg cccaggggcc
    37261 gatccccgca aatgtatcgt ccgggaacgc cgcctccagc tcggatcgct ggttgaagaa
    37321 ggagaagaat acaccgccgg ccaggtaggg ggaaagccgt atcgcgtcgg aaaggcggaa
    37381 gggcagggcc agatccaggg cggcgctcag gttggagatg gaatactccc cgacgggctg
    37441 gagagtgtaa tccagccaga gaccgggagc cgtcccgaag gggccccggt tccgaaccag
    37501 ccccgcacgg acacccaccc cgagcccgaa gaccgaggct ccgtttccca gcggcgcttc
    37561 tacaccgggt ccggcgctca gggaccaggg gcgggtctcg tcggcggata cggcggagac
    37621 gatcaggatg aacagcagga gccacctcgg aagggggcgc aaaaggcgga atcggcgcat
    37681 gggcacctcc ggtgtaaaaa accggtggat gcgggaccat ggaagctgag cggcgaccgg
    37741 cggagttcaa ggttcacggc tgcgtcagcg agggggaact acccctattc taacactatt
    37801 tcccgatatt ggaagatctc caagtcgagg ttgatctttc aggaagggtc cgggggaaac
    37861 cgtcgatacc aaaagattcc accgaccccg ggtaaatatt agtttgacag gtagaaaaac
    37921 cgatgctagt ctcatgttaa acattggacg tttaatgtcc acatgtcaat actttcagga
    37981 ggcgttcatg aagcggcggt tcattctact ggcggttctt ctggcgacgg tcggggcttc
    38041 catcctctgg gccgcgccgg tggtcctgaa actcggacac ggggcccagg cgacccatgc
    38101 gagccacatc gccggactcc ggttcgcgga gctggtgaag gaacggacca agggccaggt
    38161 cgaggtccag gtattcccga accgccagct gggcgaggaa cgggacctgg tggagggtct
    38221 gcagatcggc accgtggact tcgccgtcgt ctccaccgga cctctgggcg gtttcgtccc
    38281 ggaggtgggt gtcgtcgacc tgcccttcct cttcacgacc gctgcccacg cctacaaggt
    38341 cctggacggc gagatcggcc ggggcatcct ggacaagttc cagtcccggg ggatcatcgg
    38401 ggtggccttc tgggaaaacg gctggcgaaa cctgtccacc tccaagcgga aggtcctgaa
    38461 gcccgaggac ctcaaggggc tcaagatccg gaccatggag aacaaggtgc acatggcctc
    38521 cttcaccgcg gccggggcgt ccccggttcc catggtgtgg ggagaggtct ataccagtct
    38581 ccagcagggt gtgatcgacg ggcaggagaa tcccgtggtg atcatccatt ccaacgccct
    38641 ctgggaggtc cagaagtact acgccctgac cgggcatttc tacggctccc acgtcttcct
    38701 ggccagcaag cggaacctgg acaagctgcc caaggacgtc cagaagataa tcctggacac
    38761 ggcacgtgag ctctccgcct tccagcggga ctacagcaag aagctcgagg acgagttgat
    38821 cgtccagctc aaggccaagg gcatggatgt ctacgagatt cccaacaaga aggccttcca
    38881 ggacgccatg cagccggtgt ataagcagtt cgagtcccag ttcggcaagg ctctcatcga
    38941 caagatcctg gccgcgggcc gctgaccggc caacgtccgg ctcgggtgtc gcgagactca
    39001 gggccgcccc gcgaaggggc ggcccttgct aaaggagcga gtcataccat ggatgcgttc
    39061 atcctgggct tgaagaaggc gatagaccgg atcgtggacg ccgtggcggt cagtgccctg
    39121 tccgtcctgg tgctcgtggt ggccgcccag gtcatcagcc gctacgtggt cggacggccc
    39181 ttcacctggt cggaggaact ggcccggtac atgctggtct ggatcgtcta cctcttcgcc
    39241 gtcatcgcct tccggctctc ccggcacatg gggatagact tcttcgtcct cctgctttcg
    39301 gataggttta gagggattgt ccagggcgcc ggggacgtcc tgatcttcgg attcctgatc
    39361 cttgtcctgc ggttcggaac ggaactggtc ggcatcaccc tgccccagac ctccgcggcc
    39421 ttgggcatac ccatggggct cgtgtacctg gcgtttccag tttccgcggg gctcatggcc
    39481 ctggagctcc tattccggct gtacagccgt ttgaccggag gccgacggtg accacgacgc
    39541 tcttcctctc cttcgcggtc ctgatcctgc tgaacgtgcc catcgccttc agcctcttca
    39601 tatccgccag cctggccctg gccctgaacg gcatcaccct gatggccgtc atccagcggg
    39661 gcgtcacagc ggtggattcc ttcatcctcc tggccatccc cttcttcatg ctcgccggaa
    39721 acctgatgtc cgggggcggc atctccaacc ggatcatcaa gttcgcgaac tcctccgtgg
    39781 gtttcatccg aggcggcctg gcccacgtca acatcgcgac cagcatgctc ttcgcgggca
    39841 tcaccggcgc ggcggtggcg gacacctcgg cggtcgggtc ggtcctgatc cccgccatga
    39901 aggacgaggg ctacgacgcc gatttctccg ccgccgtcac ggcctcatcc tcggtcatcg
    39961 gggtcatcat ccccccgagc atccccttcg
//
LOCUS       DAOTEI010000008        39961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_666_length_39961_cov_6.094898, whole genome shotgun sequence.
ACCESSION   DAOTEI010000008 DAOTEI010000000
VERSION     DAOTEI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 39961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39961
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_666_length_39961_cov_6.094898"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /gene="yjfF"
                     /locus_tag="P5313_01410"
     CDS             <1..844
                     /gene="yjfF"
                     /locus_tag="P5313_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HRY79050.1"
                     /translation="VGMTLVIVAGGIDLSVGSMLALSTMLSSFLLERAGWKPGQVIPT
                     VLAVGLALGAFMGYMIHAFSLPPFIVTLAGLYLARGLCYVVSIDAIGIKNEFWQGMAN
                     ARIPAEAANSVSPSVIIALAVVAAGGFILRSTKFGRTIYAIGGNEQSAVLMGLPVART
                     KVGIYAISGFCSALSGIVFSFYTLAGYALNGRGMEMDAIATVVIGGTLLTGGVGGVVG
                     TVFGVLVYGTIQVLIMFQGTLNSWWTRIAIGFLVFLFCLLQRVFEAGRGKSGKDGSAG
                     PASC"
     gene            complement(945..2444)
                     /gene="araA"
                     /locus_tag="P5313_01415"
     CDS             complement(945..2444)
                     /gene="araA"
                     /locus_tag="P5313_01415"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HRY79051.1"
                     /translation="MIDLKTYDIWFVTGSQDLYGPETLRKVDEHSKAMAAGLDAVLPC
                     RLVWKPVRKGPDAILELVREANSDPSCAGIVTWMHTFSPSKMWIAGLLELRRPLCHLH
                     TQYNRDIPWSTIDMDFMNLNQSAHGDREHGFIGARLRLPRKVIVGHWEDPQVRERLAG
                     WMRAAAAFQDGRSLRVARFGDNMREVAVTEGDKVEAQAKFGWSVNGWGVGELVRAVEA
                     VPYAEADRRVRDYAELYEIAPDARSGEGGKRVREQARIELGLRAFLSGQGRPFGAFTT
                     TFEDLHGLPQLPGLAVQRLMAEGFGFGAEGDWKTAALVRAMKVMAAGLPGGTSFMEDY
                     TYHLEQGRQLVLGAHMLEICPSVAVGRPRLEVHPLGIGGKADPARLVFDGDSGPALNA
                     TLVDLGDRFRMIVNEVQAVPAPAPMPKLPVARVLWRPLPDLSRAAESWILAGGAHHTG
                     YSRVVKTEWLRDWAEMAGIEFLRIGEGTDPEEIRKELRWNEAAWRLGAR"
     gene            complement(2448..4127)
                     /locus_tag="P5313_01420"
     CDS             complement(2448..4127)
                     /locus_tag="P5313_01420"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HRY79052.1"
                     /translation="MKRERGLDYVLGADFGTDSVRVVVVDAADGSVAGSGVSPYRRWA
                     EGLYCDPASNRFRQHPLDHIESLEAASRAALAEASGRTGPSTAAKVRAVSVDTTGSTP
                     CLADGTGRPLALVPGFAEDPDGMFILWKDHTSVAEAERINALARGWGGEDYTKYSGGV
                     YSSEWFWAKALHVVSANPAVAGAARTIVEHCDWLSALLAGAGNLGAWRRSRCAAGHKA
                     LWHASWGGYPPAEFLARLHPRLAELAGSLGTETWPATEAFGPLCEEWAARLGVPAGIT
                     VGVGAFDAHMGAVGGDLEPGTLLKIMGTSTCDIMVGPKPADDREKLVRGICGQVDGSV
                     IPGMIGYEAGQSAFGDVFAWLRGVLAWPLRNLPGIDPGQAARMEDALLPLLEAAAAKV
                     DPERSGLTALDWLNGRRTPDADQSLKGAIYGITLGTDAPALYRALIEAAAFGSRAIVE
                     RMRQEGLRIDRVAAVGGVAKKSPLAMQVSADVLGMEIAVSSSEQSCALGAAMFAATAA
                     GLYPDILSAQAAMRAPVEKVWKPDPGRRAVYDRLYRNYRALGAYAERAAKE"
     gene            complement(4124..5182)
                     /locus_tag="P5313_01425"
     CDS             complement(4124..5182)
                     /locus_tag="P5313_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY79053.1"
                     /translation="MPNQNATVKEIARLAGVSIGTVDRVLHGRDGVAEETRENVESIV
                     RSLGYRPNVLARQLSLGKTWLFRVLAPKAGQDSGYWGLCLEGIRAAARDLSRFSVRTE
                     VVEFDRYDPAGYRKLLSGTLAEGFDGLLLAPVLPAVIGPALAALPPGTPYAFFDAALE
                     GADPVFSIGQDAYSAGRLAGRLLSLLAPGDVPLAAIDAHPEDGHIRRRVAGFESYLRE
                     AGSRRLIVRECPGFERPEVRARWFEDIFREEPGIGGLLVANSSGHLAGEWLAAAGKKD
                     RCAVVSWDLVPRNAAALREGLVDCVISQRPREQGRLGLERLYRIVAHGDPGGTVAVAT
                     EILLKENMPAPEAVEERT"
     gene            complement(5392..6522)
                     /locus_tag="P5313_01430"
     CDS             complement(5392..6522)
                     /locus_tag="P5313_01430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY79054.1"
                     /translation="MSAAEAAGPGFVTAPGFEGAARAFDGQILRGLHFGAQIEVWHGD
                     RRVLSRWGGFRDPRGARPVLGDTPFAAYSATKALTAACVHKLADEGRLDLDAPVAEYW
                     PAFAAKGKGGVTIAHVLLHQAGIPAAADLPELLSWLVPGGGERRAAARRPQHAPGEKV
                     IYHAFTGGFVLGEVIRRVSGLSAADYLKREFLAPLGMTDSFPGLPPGELRRASGIHTA
                     DPAQIGAARVFSNPLYRRLFLPAASLNTCARDLARFYRMLCAGGQLDGRRYLSKAAVE
                     RATVLRWDGPDAQSGMRIRWALGFGLGGYSPFPDRDIRHMGRGATERTFGHSGQGGCA
                     FGWADPPSGLVFAFTCNRFQDIPGAHLRFQELADACWEGVGK"
     gene            complement(6519..7850)
                     /locus_tag="P5313_01435"
     CDS             complement(6519..7850)
                     /locus_tag="P5313_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79055.1"
                     /translation="MSPKESALTPEFYRKSALLGFGLFGAQLMWMVYNTYMPIFLQAG
                     SPGFRAAAAVRGFGLSATVTGILMTLDNVAAFFLQPIMGPLSDRTRSRFGRRMPWILV
                     FAPLSALAFALIPMGTQLIPPESSGRVSELGGPFALLMGAALVMVVCMALWRTPMFAL
                     LPDLFPSALRSQANAVVNIMSGIGGILAFVVGGALFAVYQPLPFWFGALATVAAVLVL
                     FRSVREPPELAQAAEAPGGLRVLARLREIPGENKRSLVLLILTVFFYMVGYMAIETFF
                     SSFAVTRLGLAPSAAAMLLAVSYVSFLIFALPASLVAKRLGRRITISVGLLTFAAGLA
                     VIRLFPSLPVVAAMLLVGGFGWALVNVNCLPMILDTSTSEELMGTYSGLYFIATTLAG
                     TIGPILNGRVIDLAGRDYSVIFLVCPIFFVLSFLCISGVRTGEVKVSGGRG"
     gene            complement(7969..8682)
                     /locus_tag="P5313_01440"
     CDS             complement(7969..8682)
                     /locus_tag="P5313_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRY79056.1"
                     /translation="MKQFAILGLSWFGKNVLVELLDMGADVMIIDKDREVIDAYRDYP
                     VTAIVMDIVNEENLRKALPQGLDGVVIDMGDKIEASILATSYCRKLGIPRIFVKAVTE
                     GHAEILEMVGATKIVFPNREAAKRVTPLLVSSGLLNYLPVSGNLVIAELVVPDRLFGQ
                     SLLEANLRKNHGINLISVRNGDEEYGLFSPEYRFRPGDVALVSGLDESVEAFANGHGT
                     ARRAGSLMRDLGRLFGRRK"
     gene            complement(8687..10021)
                     /locus_tag="P5313_01445"
     CDS             complement(8687..10021)
                     /locus_tag="P5313_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRY79057.1"
                     /translation="MKRAFKSDAAYLLSFFLIVIFVGTFLLMLPAAWAGRPGRPVSLS
                     PIDALFTSTSAVCVTGLTTVDTADYSRFGQLVILGLIQVGGLGIVSFTSFMLILPGGR
                     LPFRRLRTIRSFSIQGVENDPFRILRSIVVSTFLVEVLGAVALAAQFARLGQADPIYL
                     GIFHAISAFCNAGFSVFPDSLERFKGEPVLLGTFSVLIATGGIGFIVLQDIARRIRGK
                     KRILSYHSKLMLSITAILILAGAGAFWILEKDHAFAGMSGPVRAMNALFQSVTPRTAG
                     FNAVHQAGLRQPSKFITILLMFIGGAPGSIAGGVKIATAYVVLLVMLKRANERGEINS
                     FRRRLTPETINSAVIYFIKAAFLLIFAAGILSVVEAPRGVDFGHIAFEVTSAFGTVGL
                     SLDFTAGLSTAGKLVIIGTMFTGRVGLLAFLFLGGSTRAGNFVYPEAEILIG"
     gene            complement(10023..10709)
                     /locus_tag="P5313_01450"
     CDS             complement(10023..10709)
                     /locus_tag="P5313_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79058.1"
                     /translation="MVIPVLVVDDEVQIRKLLRLYLEKEGYEVFEADGLEEGLRQFGA
                     VKPGVVLLDLGLPDGDGRDLLLAVRRRGDVPVIVLSVRDSEEEITGLLDAGADDYLIK
                     PFGVGELMARIRVAVRHRGLGSPEGRPLRVGLLEVRFDTREAFRDGAEEHLTPTEWSI
                     LEMLVRNSGKILTRNQILREVWGPDMAGDYNSLRVYVNQLRKKIEAEPSVPRTLLTEP
                     GVGYRLKLES"
     gene            complement(10703..12625)
                     /locus_tag="P5313_01455"
     CDS             complement(10703..12625)
                     /locus_tag="P5313_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRY79059.1"
                     /translation="MEEPISSRSESGLGDHFLVAVTGSRNSEYLIRWTDAAARRQGAA
                     WTVLHVHDPECDEDPAGVERNLALARTLGAEILSAVDRDVASCIVRHARVKDAGTLVI
                     GKAEEADRSFLGRRSVMEDILRESGDLDVVILRGKAPVPSVRRPLKIRDSSGPLRGVP
                     LALAALASITLLGHLAQPALGYRSVSILYLLVNMTLPFACGRITVLASAALSSLLWNF
                     LFIPPRLTFSIASLEDLLMFTAYFLAAFAGGLLTSRLKEKESALSLRERRMAFLYGFT
                     RAVSRERDIAGLARLASDYLERHMRVRASVFLRGTDGTLDTGGPVRGGPPTAPGFDPS
                     AAARCFQSDVCVADDPGRLYIPLGSQDGVLGILYAAGPGERALDGENREVLSALAGNL
                     ALALERELLAEENERNKLAGESARLSRILLNHVSHELRTPLTTITGSVSALQAGAAED
                     PALRGELLSETMIAAGKLNRIVEDLLAMSRLEAGRLVVRPETAYVGELIGAAREGLES
                     DLGGRTVTLEESARDAEITADPVLMVQVFRNILRNFSAYTPPGSTLRVGSESVPGATV
                     VRFSDDGPGVSERELPLLFDTFFRGSRTGARPGCGLGLSICRGIVEAHGGSARAARSD
                     RGGFMIELRLPRKEES"
     gene            complement(12725..14092)
                     /locus_tag="P5313_01460"
     CDS             complement(12725..14092)
                     /locus_tag="P5313_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRY79060.1"
                     /translation="MDHARLRKDATDIFAAGVASVDPRPMVRDALKPEEGRLTLAAGG
                     RPLSWDLGRFDRILVLGYGKAGARMARGAEEVLGKRLSGGVVAVKAGHAEPCDRVRIL
                     EAAHPVPDETSVRAARAVMEAARGADERTLVLVLVSGGGSAILCAPYDRDGRTLSLAD
                     KAAVTRELLACGADIREINAVRRHLSAVKGGRLAEILAPASVVSLILSDVVGDELASI
                     ASGPTVPDPSTWADALGIIRRFGIEKKIPAAAADLLRDGAAGLLPDTPKPGDGIFRSV
                     ANIVLGSNRRAAMAAAARARQLGYRTLYLGSRITGEAREIARAYLGMALDCAADGTPR
                     SRPACLIAGGETTVTLRGTGTGGRNQEMALSLLAGPSDAEGRLAQRVCFLSAGTDGND
                     GPTDAAGAFADTGLLEAARGAGLKPRDYLAANDSYPFFDRIGGLLRTGPTNTNVCDLQ
                     VVLVR"
     gene            complement(14103..15434)
                     /locus_tag="P5313_01465"
     CDS             complement(14103..15434)
                     /locus_tag="P5313_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRY79061.1"
                     /translation="MVSGTVALLLLVVAVVAIVFLTGKYKVNAFLVLIGVAFAFGLAI
                     GLPALDVVGKIKTGFGGTLTNIGIVIVAGTIMGTILEKTGAALSMTQAILKLVGKNRA
                     PLAMNAAGYVVSIPVFCDSGYVILNPLNKALAKETGTSMAVMAVALATGLYATHTMVP
                     PTPGPIAAAGALSADLGRVILFGLIAAVPASLAGLFWATKVAKKYQIEPDVEESYSDI
                     VKKYGKLPSTFLSFLPIVLPIVLILLKSVAEFPSKPFGDQSLQKLIRFIGDPVTALMI
                     GVFCSLLLVKKGELGKAVDGWLGQGIKDSAIILVITAAGGSFGAMIAASPIVEFLKGN
                     LTSLSLGVFLPFIIAAALKTAQGSSTVAIITTSTILAPMMASIGLDPALAVVAIGAGA
                     MVVSHANDSYFWVVSQFSGMSVNTAYKAYSSATAVEGVVAALVVAVMSLFV"
     gene            complement(15516..16766)
                     /locus_tag="P5313_01470"
     CDS             complement(15516..16766)
                     /locus_tag="P5313_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HRY79062.1"
                     /translation="MSPDREASGGTSNPATPGERLRLDAETAGAFLRKLGERLDYNIN
                     IMDREGVIIASRDPARVGTYHAAARHLLESGSREERISPGDHLPEGTKPGVNLPLEVR
                     GEVLGVVGVTGDPQVVGDLAQAVKTSVESMIELESMREAVLNRRTRKSSLMAYLLYEE
                     AVPESAVLALAGKLGYDPAIERAALLLRLPPGKGPEDALAQAKARGAHDAQDIASALP
                     DGSLLVFRRLGTERVEAPEERVPGILAAYRGEVQAYCGAFSRAAGIPKVTCFAGSPQG
                     DFSRYRGSYRQALWLASRFPVPPGDGPVLILDHLDDYFLARTPRIVLDDALASIAARA
                     PAELRRTLAPTLKALEASGFNAKEAAARLGVHRNTLSARLARLSSFLGCDPRADAGAL
                     SLSRSMAAYLKLRGGTVQDAQEHL"
     gene            complement(16756..17868)
                     /locus_tag="P5313_01475"
     CDS             complement(16756..17868)
                     /locus_tag="P5313_01475"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRY79063.1"
                     /translation="MKALFAYYTGTGNTLRALEKVAEGLEASGWEAEWLEIRAGGPSL
                     GSALQCLRENDLLVLGFPALGFSAPRPVLRALRSLPGLPGLRAAVLCACGATAAKGRV
                     VNGWSGTAVPEALRILVRKGAVPVGSVEASYPENWTQVSPPEDEAARAAMTSLGDGQA
                     REFGVRLAAALAGGGTDSPGLPAGSVRRGPVSRALGPLLARGFRTFGRRLLARLFVAD
                     GSCTSCGLCARTCPSAAIRMTGARPAWTTACSGCNRCINLCPERAIQTSYARLVLVLG
                     LNVAALFLSGPAARALLETASGRGGRSGGIGAFAAGLALYLGFSLLQAGPLDAVLRAL
                     ERSGAFGSLFSRGFTQRFGRYRAPGFQPGRNVRDVP"
     gene            17997..19001
                     /locus_tag="P5313_01480"
     CDS             17997..19001
                     /locus_tag="P5313_01480"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HRY79064.1"
                     /translation="MHPRPKLFVSRCLGFEACRYNGAVIEDPLVAKLAGFCDVTTACP
                     EKDTGLGVPRDPVRLARAGGGIRMIQPASGRDHTDAMTAFCAAKAAELAGTAPGSARP
                     PEGLPAVDGAVLKSRSPSCGMKDVKVYRSTEPGAMADVGIGLFGQAVLSALGGAPVED
                     EGRLKNYTIREHFLSGLFARARFRAVAESGGPRELSEFQARHKLLFLAVNQASMRRLG
                     RIAANLERHPFPAVLSEYGAELGTMFGSPAKPGGVINTALHAFGGFSERLSPLERKYF
                     LDAVEEYRDERIPASALFTLLKAWAVRFEVRYLLDQVFLDPFPKELVEITDSGKGRDR
                     "
     gene            complement(19051..19857)
                     /locus_tag="P5313_01485"
     CDS             complement(19051..19857)
                     /locus_tag="P5313_01485"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRY79065.1"
                     /translation="MPRARALYIPHGGGPLPLLGDPGHAELVGGLRGLGRDLGRPSAI
                     LVVSAHWEEPEVRVIGGPAPELLYDYYGFPEEAYRIRYPAPGDPALAGRILETLSAAG
                     IPARPEEARGFDHGVFVPLALMYPGADVPCVQVSLIRGLEPRAHLDLGRALTALQGED
                     LLILGSGFSFHNMREFFSAAPGSPDPRNEAFQAWLTETCASPDLPAGERDRRLAEWER
                     APHARYCHPREEHLLPLHVCAGFGGRPASRSWSWTVLGKKASAFLWEPGA"
     gene            19969..20706
                     /locus_tag="P5313_01490"
     CDS             19969..20706
                     /locus_tag="P5313_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79066.1"
                     /translation="MKPLVLLRTLVRTAGILAGAFLVFTGLANLPRMFLGIGVILAGL
                     GLGALVNAALRKAGQYIPPEIVADPGIRNPVLRFLIQPEILIVVFGFFFNFFWEVSQI
                     PLYEGINRGIPYFGENTVEMKLFFVRTFWRAAFLDALLLVGAHLLTLAILRERFWFSK
                     GGRLFGIRSLTVRPWVGFGISTLICVAFLIYLEISAFDQGLWAYSRLMPTLFTKIGLT
                     PVGGLLVSPTLVFLLTRRVSRPGPPLS"
     gene            20703..21329
                     /locus_tag="P5313_01495"
     CDS             20703..21329
                     /locus_tag="P5313_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY79067.1"
                     /translation="MTEIEIRDGRPEDAPVAALLIREAGDRLMDFLAGFRGRAATEEV
                     LEGLWNLPGTRFSREAARVALTDGEPAGILVGYPREAMAGLDLGTGRALLGLRGLALA
                     AGVLRHPVLTWRMSTLPEAEPGDWYICVLATREACRGRGIGAALLSDAESRARRHGAA
                     ALSLMVSEGNAPARSLYVKFGFEERGRFTVAGRTSLRMGKALEMLEPR"
     gene            complement(21399..22031)
                     /locus_tag="P5313_01500"
     CDS             complement(21399..22031)
                     /locus_tag="P5313_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79068.1"
                     /translation="MAENGREEDPRIRKTRRAIVQAYLDLCSEGGGPASTVASIAERA
                     EVNRATFYRHFEDKEDLAERGLGLYLGELLAEIDPESVGRARTGSRPADDSGPRERIR
                     ARVERFFRVLQDNASLFRPLLSGRAGSRVFLGIEEYFSAYLRESRLARLSGIRLSLPE
                     PLAARALSMMILGLAASWLEDSRGLSPGDLAGAYVDFLFEGIFKNSGKEG"
     gene            22232..23266
                     /locus_tag="P5313_01505"
     CDS             22232..23266
                     /locus_tag="P5313_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRY79069.1"
                     /translation="MDERRTLKIGLVQNSPKPGAAIENLMDARPLIEAAAAAGADLIA
                     LPELSASGYAPTRGIWRSAEPADGPTERWLGETADALGVYLCAGYLETDGTDFFNTYA
                     VAGPEGRLLGRVRKETPSMGETYLFQGETGSHVVETPIGRIGIGICNDNHMAYLPGRL
                     RAGGAELLLMPHAWPLPTEPSRLVSREDIERQRANMTELPARYARGLGIPAAFTNLSG
                     TLDLGGERSPGFLGALLPPPEKYGFPGYAAIHAAGGEILRSRADGEGWIVGEVVLGTG
                     MRPEASMPAYGRRVYPGNSDGGILALYESLGRRSYARSRDRRTAARAAFEAGRTSLRM
                     GKALEMLEPR"
     gene            23356..24450
                     /locus_tag="P5313_01510"
     CDS             23356..24450
                     /locus_tag="P5313_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY79070.1"
                     /translation="MNPTRSRALLAVVLAVLAIAAAAAQARPSGTAPALPYPRTSSEW
                     AAFLAPDPRFVELGGIPEPMPTRKLAEAGLVASGVPPERMEYYIGKLRETFEALRARS
                     EYIQDPALRAESILPFLHRTLFKKYEENSTTVDGVIDTGYFNCVSSAVVYLLAARVFV
                     LPMKGVQTPDHAFCVITVNGRDIDVETTNPFGFDPGTSKKFQSTFTKTTKFAYVPPQD
                     YARRKTITEKDLVGLILHNRGVDMQRRGRHLEALRLAVDRATGFPGPDAAEFLAACAQ
                     NVLAELFDRRDWPGALDMAEGVRKLAPADPRLRELYTLAARNYAVDAHNRFAERYNAR
                     DTAGARAILEQALRRLPGDPILTQDLEALR"
     gene            24473..25360
                     /locus_tag="P5313_01515"
     CDS             24473..25360
                     /locus_tag="P5313_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRY79071.1"
                     /translation="MGTLRIGTCSWKYPSWKGLVYSSREPGNFLAEYARKYDTVEVDQ
                     WFWSLGRSGAGLPRRETVLEYDAAVPPEFRFTVKCPNALTLTRPYARKGETAEPNPRF
                     LDPGFFHGFLDALAPLVPKVGLFLFQFEYLNKDRIPGREAFLERLGTFLESLPGDLPY
                     GVEIRNPRWIDAGWFDFLAEHGAAPVLLQGYWMEDLADILARHGGRIRGRACLRLHGE
                     DREGMEERTGEDWSRIVRPKDGDLARIVPELATLVEALDEVYLNVNNHYEGSAPLTID
                     KIRRSLHLSEGEQHGFRAP"
     gene            25341..26279
                     /locus_tag="P5313_01520"
     CDS             25341..26279
                     /locus_tag="P5313_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY79072.1"
                     /translation="MDSGRPDIRVLGRRAAAASLRFLASSLATFLVILFMAGLPLLIS
                     AKDARVEIDPERVLAAARGFLASALDGTLRTFKIGRTAWDLREIYPKFLSVSFLYTAI
                     PGAAGILLGTVAGAAARSRRRGTLDRLTDLALGTPDFLLILAVQLGAVWILDTLGIDL
                     SLGGNSRLALLPLSVMGVYPFFLARRCAAEASREAETSDYIAFARAKGLPEGWILRRH
                     LGAAVIPRLEAELPVIVSFMQGSLFITERAFALPGMARLLFDSAFSGQRKHMMWTIYQ
                     YDHVVLSLTGLAVSGLLVFAALRLGLGAARKVLTRE"
     gene            26272..27177
                     /locus_tag="P5313_01525"
     CDS             26272..27177
                     /locus_tag="P5313_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79073.1"
                     /translation="MNRRLAAGLLLLALLLGLALFSDSIAPHERGFMKAVRYDTVDGQ
                     EVMLVSPEPPSRFFLFGSDGWGYDLFSEMIHGLGWTLSIVFATALLRSTAALTAGLAL
                     GALRPTGLRRRRFSPLASIPGFILAAFVLYPVTINSRLPPVALFAVQSSLLALVELPA
                     LAESFAARSADVLGRPFVEAARTAGAGVPWILRYHVLPFAFTDWLESLPVQALSVAAL
                     VGKLGVLRIFVGGTILTVDPVIFLPAKGEWLGLLGYYYTGVRGQPWLFLAPFAGWMLI
                     LTCVGLLSSGTKAAWARSRRIEVPE"
     gene            27339..28448
                     /locus_tag="P5313_01530"
     CDS             27339..28448
                     /locus_tag="P5313_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HRY79074.1"
                     /translation="MRTVRSILMVLLALSLVAMPVFAQKDYKIGIMTGTVSQNEEEFR
                     KAEAVRNALGANRVVLATYPERFMQEQETTISNMMAMASDPKVKAIVMVQAVPGAAAA
                     IDRVKAVRPDILFILGVPAEDPDMIAKKADIVLQTNDLARGEQIAQQAKKLGAKTLVH
                     YSFPRHMSYEHLSKRRTLMKEAAEKIGLKFVEQDAPDPTGEGGVPATQQFIMEDVPRK
                     VVQFGKDTAFFGTNCSMQEPLIRQVVAQKAVYPVQCCPSPFHALPGALGIAVPPEKKA
                     DVPWILSEEKSKIKAAGMNGRMSTWPVPVNMMYIEAGVKYAQAYVEGKTKGKAKVDPA
                     LLTKIMSEIAGGKVELTNYKDYPNFFMYLSDFYNF"
     gene            28566..30122
                     /locus_tag="P5313_01535"
     CDS             28566..30122
                     /locus_tag="P5313_01535"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY79075.1"
                     /translation="MKGISKEYYGTKVLKGIDLEIRPGEVHGIVGENGAGKSTLMNIL
                     FGMTVIHSTGGYQGEIEIAGAKADIKSPFDAMKLGIGMVHQEFMLIPGYTITENIKLN
                     RETTKDNPVSRVFGPKLKTLDMASMGRDARKALDHLEMGIDEWARVAGLPVGHMQFIE
                     IAREIDKSNIKLLVFDEPTAVLAESEADNLLAAVRKLSASGIAILFITHRLHEIMAVC
                     DGITVLRDGSLVAHKRREETTIEELARLMVGRSIGDMIRNPAKSLPDRNALEIRNLRV
                     QMPGEAAKGVDLDVKDGEILGIGGLAGQGKIGIANGILGLYPAEGEVRFHGEPLPLNS
                     TREALKRGLAFLSEDRRGVGLLLDMSVEMNIAATAVEIQGKFLRRFGPFSQIDGKAVR
                     AHALETIKALDIRCTGPLQPVRRLSGGNQQKVCIAKALVLGPRLLFVSEPTRGIDIGA
                     KRLVLETLVDLKERLGMTIVITSSELAELRSVCDRIAIVHDGRIAGILNPDDDDAEFG
                     LMMAGDYGKR"
     gene            30166..31182
                     /locus_tag="P5313_01540"
     CDS             30166..31182
                     /locus_tag="P5313_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79076.1"
                     /translation="MRSFGAPRIIIIVFLVSLWITSTLLKIPFPAVLSDSLVRLGMNA
                     VLTLAMLPSILSGTSLYFGLPLGIICGLLGGVMSIELDLRGFEAFFGAVAMSLPLAVL
                     VGWGYGWLLNRVKGSEMTVGTYIGFGFVSLMSFGWVTLPFHHPEMAWAIGKGIRVTIS
                     LEGRFSGVLNNLWSFDILGVRVPTGALAFSALCCLLVWLFFRSRSGLAMKSAGMSPKF
                     AEASGINVNRMRIRGTILSTVLGAVGILVYSQGFGFLQLYQAPMYMGFIAAASILIGG
                     ATLKTGRISHVIIGTFLFHSILVVALPVANTVSQLGSLAEISRSVVSNGIILYALAQK
                     GGAE"
     gene            31179..32468
                     /locus_tag="P5313_01545"
     CDS             31179..32468
                     /locus_tag="P5313_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79077.1"
                     /translation="MSHDANPHGTAGTKGAVRRFFTNNLVTLVFLFIGIAGLRVSGLA
                     LPFFIVDIVDRVARNGFLVLALIIPVLAGLGLNFAIVLGAMAGQATIIFMTHWGIGGI
                     WGILLCMAISFPIALALGVLTGRLLNKARGKEMITTLILGFFANGIYQLVFLILVGTL
                     IPMKNETLVLSSGVGIRTTIDLTDGLKFGLDDLLRIPLPLFAILCGAAVLGSFPVRSV
                     LRKRALAAREKTPLRDGVLALTGLFLAGWGIKNLVVESMYSFIRVPAGTFLVLGALCL
                     FILFFTRTKLGQDMRTVGQDRHVAEVSGIDTDRIRITAMVMSTVLAAWGQIIFLQNLG
                     TFSTYGSHEQVGMFAIAALLIGGASVAKATIGQAVVGLFLFHTMFIVSPLAGRALFGD
                     AQLGEYFRSFVAYGIIGLSLALHSWKKHMQQKQGAGG"
     gene            32481..33557
                     /locus_tag="P5313_01550"
     CDS             32481..33557
                     /locus_tag="P5313_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRY79078.1"
                     /translation="MREAVGRRRPPSGAGSSRRSEYPRLADFTTVFLSILLEAFPFVL
                     LGTLISSAIHILVSAETIARILPRSRLAGLLAASAAGFAFPICECANVPVTRRLLAKG
                     LPAPLAVTFLLAVAIVNPVVLFSTWVAFGGNLGVVLLRGLLGMGIAVLTGWIVGSLPV
                     GRQPLRDTAPGGAGEEQGWEHGPDHGPGCDCGHDHPHDHDAGHGGNRGADTGRAGRRG
                     AADAALEILTHAGAEIYSVGRYLILGAAVAALMQTILPRAALVGVGAAPLLSVAALMV
                     LAYLLSLCSEADAFIAATFAGYFTPGAVLAFMVYGPMMDVKNTLMLLDGFKPRFVAIL
                     LALVTAACLGAGFLVNLLASGGVG"
     gene            33554..34339
                     /locus_tag="P5313_01555"
     CDS             33554..34339
                     /locus_tag="P5313_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HRY79079.1"
                     /translation="MKRTRISPGAAAWAAILLAWSLWMLRLLVSEDVLLYVHPRMRVF
                     VWFAVLGFLVLSAVTARSALGGTVGRAPRAGYALFLVPLTLAVAGGPRALGAGAAENR
                     LLRAAAPGTAQSAAPGRTERTLDSILASADPGTVLSFEDDFHSAMAADMAAAPERYEG
                     RRLSLVGFAHRVPGLPADRVFVTRLLITCCAADAEPVGVLASWPGAAGLRGGEWMRVT
                     GTGKRTAYRNPYTGKDSFVAMVEVDEVVPGEQPASAYLYPGQF"
     gene            complement(34347..35066)
                     /locus_tag="P5313_01560"
     CDS             complement(34347..35066)
                     /locus_tag="P5313_01560"
                     /inference="COORDINATES: protein motif:HMM:NF027487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HRY79080.1"
                     /translation="MEGPAVAERLARLNPGLAVFGPGDPRLARYGRIAELPGLAALAE
                     AAAPRIPSNLEANAYTASDPDLEARPEAAAFGPVFGFYPLQVGWNAGPNTRLNGLEWH
                     ASPEILVAVTDLALLLGRVEDIDWDSPGGPAYPSNRVGCLVLSAGTALEIHPGTMHLA
                     PCRLSDRGFKSLVVLPRGTNEILSEEEKAAAAKRAAAGDPRARLLFMRNKWIITHPER
                     KVLVDKGAFPGITGPNVEVRY"
     gene            complement(35066..36568)
                     /locus_tag="P5313_01565"
     CDS             complement(35066..36568)
                     /locus_tag="P5313_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRY79081.1"
                     /translation="MKIEADRTFRNALFLALAAAVCAALVVLPPPASLAQTIAKAVDA
                     ATGKPAYPASVQGGEAAMSALGVLAFCLILWVTEALPFHITGMLSLGLLTFLKVGKYK
                     EIISTGFGNDIVVFFIGVLLLGAFITKSGLGKRISTVILSVTGNDTRLIILGFLIAGT
                     LLSMWITDMAVAAMLMPLARAILREEGAEPMKSNFGKGLMIAVAWGALIGGIATPAGT
                     GTNPVAMQFLASMADIHLTFLDWMAYGIPSALLILGPAWGILLAFFPPEMRKLKVSKE
                     KLREELRDMPPMNREEKLTLAVFVLTVLIWILTPLLQKPLGMVIEISLPALLAACLFF
                     LPGMSRTKWKEVEGDVDWAGILLIVSGLSMGTYLYKTGAAEWLAVILLGRIGSVPLFA
                     QLFLVTLGVCLLKVVFSSNTVTATILIPLIIGLGKALSLNVSAITLAAGLTSSLAFIL
                     VTSTPTNVIPFNAGYFGIKDMAKAGLVLTVVASLVIAVTFLVVGGIRGDF"
     gene            complement(36578..37474)
                     /locus_tag="P5313_01570"
     CDS             complement(36578..37474)
                     /locus_tag="P5313_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRY79082.1"
                     /translation="MSVFIDRSDRVLVQGITGGEGTFWTKHMRDLGTDVVAGVTPGKE
                     GQSVEGVPVYHTVRRAVAEHPADAAMLFVPPRFTKDAVLEALDAGIRKVVTIADGIPL
                     HEALEIRAAARSCGARVVGGNTSGVISPGRAMMGMFPYWIERVYRPGRIGVMTRSGSL
                     TNEVTAMIVRAGFGVSSLLGVGGDSVPGTRFAEFLPDFQADSGTDAVVVIGELGGTME
                     EEVAEAIESGVFCKPLVAFLGGRNAPAGKRMGHAGAIVTGGKGTVEGKAAALKRAGAA
                     VADRPRAVGDLLREILGDPAHS"
     gene            complement(37471..38628)
                     /locus_tag="P5313_01575"
     CDS             complement(37471..38628)
                     /locus_tag="P5313_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HRY79083.1"
                     /translation="MKLHEHEAKDVFKAEGIPVPRSVLATDPEGVARAAREAGFPCVL
                     KSQVLRGGRGKAGLIKLVRSEPEARDTAAALFASPHGVRRILVEEAVDIDRELYLSIT
                     VDPAAAEAVVLACSEGGVEIEDLAARSPEKIVRERVPLAEGLSPYRARNVAYALGLSG
                     DAAKKTAGILEALWRVFRKNDAELAEINPLFLTKGGGVVAGDGKLAIDDNSLFRHPGR
                     EPGRDYYDSDAAFEAAAEGIPYLQFDGDISLMCAGAGLTTVVYDLIVDEGGSVANYLE
                     FGGPNYKKAVRAMELCLKNEPKVILIVTFGTIARADVMAEGIADAVKRLAPKCPIVAC
                     IRGTGEEKAVETLRSLGLDPLFDTEEAVRKAVALAAAARGSGAAPGAGGSK"
     gene            complement(38636..>39961)
                     /locus_tag="P5313_01580"
     CDS             complement(38636..>39961)
                     /locus_tag="P5313_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY79084.1"
                     /translation="EVCLAVGWSVYKDENIARLARAAVDETGMGVYEDKITKHKNKVM
                     GVLRDIRTAKSVGLIERDEAKGLLKFAKPVGVVGALAPVTNPTATPSSNGLSILKGRN
                     AVIFAPHPKAKGASKLACEFMRQALRSVGAPEDLVQCIEEPSIERTGELMRQVDLVVA
                     TGGGPMVKAAYSSGTPAYGVGPGNAVQLLAEDADPEDAASKIAVSKAFDNATSCSSEN
                     SVVACGNVYDAFVRAMRTKGAHLCSPAEKEKLRSWLWPVGPDGKEALNPKVIARSAES
                     IAAGAGIPVAPGTKILMVEAELPRPGGPAPEKWAQEKISPVLALWKCPDFRSGLEAVV
                     LITDACGTGHSSGIFTSREDNILAMGETLRSSRIMVRQGMASGNGGTFANGMPSTVTL
                     GCGTWGGNITTENIHWKHFVNVTWLALPLAPNRPSDEEIFGSLWAKYGK"
BASE COUNT         6347 a        13683 c        13727 g         6204 t
ORIGIN      
        1 cgtgggcatg accctggtca tcgtcgcggg ggggatcgac ctgtccgtgg gctccatgct
       61 cgccctttcc accatgctct cgtccttcct cctggagcgg gcgggatgga aaccgggcca
      121 ggtgataccg accgtcctgg cggtggggct ggccctgggg gccttcatgg ggtacatgat
      181 ccacgccttc tccctgccgc ccttcatcgt caccctggcg ggactctacc tggcccgggg
      241 cctgtgctac gtcgtgagca tcgacgccat cgggatcaag aacgagttct ggcaggggat
      301 ggcgaacgcc cggatccccg ccgaggcggc caacagcgtc tcgcccagcg tgatcatcgc
      361 cctggccgtc gtggccgcgg ggggcttcat cctccggtcc accaagttcg ggcgcacgat
      421 ctacgcgatc ggggggaacg agcagtccgc ggtcctcatg ggcctgccgg tggcccgcac
      481 gaaggtcggg atctacgcga tctcgggctt ctgctccgcc ctctcgggga tcgtgttctc
      541 cttctatacc ctggccggtt acgccctgaa cggtcggggc atggagatgg acgccatcgc
      601 cacggtcgtc atcgggggga ccctgctcac cgggggagtc ggcggagtgg tgggtacggt
      661 gttcggtgtc ctggtctacg gcaccatcca ggtgctcatc atgttccagg gcaccctgaa
      721 ctcctggtgg acccgcatcg ccatcggctt tctggtcttc ctcttctgcc tgctccagag
      781 ggtgttcgag gcgggccgcg gcaagtccgg taaggacggg tccgccggac ccgcgagctg
      841 ctaaaaaaac cgggactgtc caaaaagctc ggatagctta ttggacaccc cggcctcttg
      901 ccgccgcccc aggcctcccg ggagcggcgg cttctttttc gcgcctatct cgcgcccagg
      961 cgccaggcgg cctcgttcca gcgaagctcc ttccggatct cctcggggtc cgtaccctcc
     1021 ccgatccgga ggaactcgat ccccgccatc tcggcccagt cccggagcca ctcggtcttc
     1081 acgacccttg aataccccgt gtggtgggct cccccggcca ggatccagga ctccgccgcc
     1141 cggctcaggt ccggcagggg acgccagagg acccgggcca cgggaagctt cggcatgggg
     1201 gccggagcgg gaaccgcctg gacctcgttc acgatcatcc ggaaccggtc ccccaggtcg
     1261 accagggtgg cgttgagggc gggccccgag tccccgtcga agacgaggcg ggcagggtcg
     1321 gccttgccgc cgatgcccag gggatggacc tccaggcggg gccggccgac ggccacgctc
     1381 gggcagatct ccagcatgtg ggctcccagg accagttggc gcccctgctc caggtggtag
     1441 gtatagtcct ccatgaagga ggtgcccccg ggcaggcccg cggccatgac cttcatggcc
     1501 cgcaccaggg cagcggtctt ccagtccccc tcggcgccga acccgaaacc ctccgccatg
     1561 aggcgctgga ccgccagccc gggcagctgg ggaaggccgt ggaggtcctc gaaggtcgtg
     1621 gtgaaggcgc cgaagggtcg gccctgtccg gacaggaagg cccgcaaacc cagctcgatg
     1681 cgggcctgct cccggacacg cttccccccc tccccggacc gggcgtccgg ggcgatctcg
     1741 tagagctcgg cgtagtcccg gacccgacgg tcggcctcgg catacggcac ggcctctacg
     1801 gcccggacga gctcgcccac gccccagccg ttgacggacc agccgaactt ggcctgggcc
     1861 tccaccttgt cgccctcggt gacggccacc tcccgcatgt tgtccccgaa gcgggccacc
     1921 cgcagggaac ggccgtcctg gaaggccgcc gcggcccgca tccacccggc aaggcgttcg
     1981 cggacctgcg ggtcctccca gtggcccacg atgaccttgc gcggcaggcg caggcgggcc
     2041 ccgatgaaac cgtgctcccg gtcgccgtgg gcggactggt tcaggttcat gaaatccata
     2101 tcgatggtgg accagggtat gtcgcggttg tactgggtgt gcaggtggca cagggggcgc
     2161 cggagctcca ggagccccgc gatccacatc ttggaagggc tgaaggtgtg catccaggtc
     2221 acgatccccg cgcaggacgg atcggagttg gcctcccgga ccagctccag gatggcgtcc
     2281 gggcccttgc ggaccggctt ccacaccaag cggcagggaa ggaccgcgtc cagccccgcg
     2341 gccatggcct tggagtgctc gtccaccttg cgcagggtct ccggaccgta gaggtcctgg
     2401 cttccggtca cgaaccagat gtcgtacgtt ttaaggtcga tcacggctca ctccttggcg
     2461 gcccgctcgg cgtaggcgcc gagggcccgg taatttcggt acaggcggtc gtagacggcg
     2521 cggcgtccgg gatcgggctt ccagaccttc tccaccgggg cgcgcatggc ggcctgggcg
     2581 gacaggatgt cgggatagag acccgccgcg gtcgccgcga acatggccgc cccgagggcg
     2641 caggactgct cgctggagct caccgcgatc tccatcccca ggacgtccgc ggagacctgc
     2701 atggccaggg ggctcttctt ggccaccccc ccgaccgcgg ccacccggtc tatcctgagc
     2761 ccctcctggc gcatccgctc cacgatggcc cgggagccga aggccgccgc ctcgatgagg
     2821 gccctataga gggcgggggc gtcggtgccc agggtgatcc cgtagatcgc ccccttcaaa
     2881 gactggtccg cgtccggggt gcgtcggccg ttgagccagt ccagggcggt gaggcccgac
     2941 cgctccggat cgaccttcgc ggccgccgcc tcgagcaggg gcaggagggc atcctccatg
     3001 cgggcggcct gccccgggtc gatgcccggg aggttgcgca ggggccaggc caggaccccc
     3061 cggagccagg cgaagacgtc cccgaaagcg gactgccccg cctcgtaccc gatcatcccg
     3121 gggatcacgg agccgtccac ctggccgcag atgccccgga cgagtttctc ccggtcgtcc
     3181 gcgggcttgg ggcccaccat gatgtcgcag gtgctggtgc ccatgatctt gaggagggta
     3241 cccggctcca ggtccccgcc cacggccccc atgtgggcgt cgaaagctcc cacccccacg
     3301 gttatcccgg cgggcacgcc caggcgcgcg gcccattctt cgcagagggg gccgaaggcc
     3361 tcggtggcgg gccaggtctc ggtgcccagg gacccggcca actccgcaag gcggggatgg
     3421 agccgggcca ggaactccgc gggcggatag cccccccagg aggcgtgcca gagggccttg
     3481 tggcccgcgg cgcagcggct cctccgccag gccccgaggt tcccggcccc cgccaggagg
     3541 gcggagagcc agtcgcagtg ctccacgatg gtccgggccg ctcccgcgac ggccggattg
     3601 gccgacacca cgtggagggc cttggcccag aaccactcgg acgaatagac cccgcccgag
     3661 tatttcgtgt agtcctctcc cccccagccg cgggccaggg cgttgatgcg ctccgcctcg
     3721 gccacggagg tatggtcctt ccagaggatg aacatcccgt cgggatcctc ggcgaaaccg
     3781 ggcaccaggg ccaggggcct gcccgtcccg tccgccaggc agggggtgga gcccgtggta
     3841 tccacggaga cggcccggac cttcgcggcc gtcgaggggc cggtccgacc cgaggcctcg
     3901 gccagggcgg cccgggacgc ggcctcgagg ctctcgatgt ggtccagggg atgctgccgg
     3961 aaccggttgg aggccgggtc gcagtacagg ccctcggccc atctccggta ggggctgacc
     4021 ccgctccccg cgacggaacc gtccgccgcg tccaccacca ccacccgcac cgagtccgtg
     4081 ccgaaatcgg cgccgagcac gtaatccaag ccgcgttccc tcttcatgtt cgctcctcca
     4141 ccgcctccgg ggcgggcatg ttttccttca gcagtatctc ggtcgccacc gcgacggttc
     4201 ctcccgggtc cccgtgggcg acgatccggt acagccgttc caagcccagc ctcccctgtt
     4261 cccgggggcg ctgggagatg acgcaatcga cgaggccctc ccgtagggcg gccgcgttcc
     4321 ggggcacgag gtcccaggag acgacggcgc agcggtcctt ttttcccgcc gccgccagcc
     4381 attccccggc caggtggccc gatgagttcg cgaccaggag gccgccgatc ccgggctcct
     4441 cccggaagat gtcctcgaac cagcgcgccc ggacctcggg ccgctcgaag cccgggcatt
     4501 cccggacgat caggcggcgg cttcccgcct cccgaagata ggattcgaag cccgccacgc
     4561 ggcgccggat gtggccgtcc tcgggatggg cgtcgatggc ggccaggggc acgtccccgg
     4621 gagccaggag ggaaagcagg cggccggcca ggcggccggc ggaatacgcg tcctggccta
     4681 tggagaagac ggggtccgcc ccttccaggg cggcatcgaa gaaggcgtag ggagtcccgg
     4741 gagggagggc cgcgagggcc gggccgatca cggccggcag gaccggcgcc agtaggaggc
     4801 cgtcgaagcc ctccgcgagg gttccggaca ggagctttcg gtagcccgcg ggatcgtagc
     4861 ggtcgaattc gaccacctcc gtcctcaccg agaaccggga caggtcccgg gccgccgccc
     4921 ggatgccctc caggcagagt ccccagtacc ccgaatcctg cccggccttg ggggccagga
     4981 cccggaacag ccaggtcttg cccagggaga gctggcgggc caggacgttc ggccggtacc
     5041 caagggaccg cacgatggac tcgacgttct cccgggtttc ctcggccacc ccgtcccggc
     5101 cgtggagcac ccggtccacg gtcccgatgg acacgccggc caggcgggct atctccttga
     5161 cggtcgcgtt ctggttcggc acggttggac ttccttaccg tgtacgtaca cgataaggat
     5221 ttatacccag agtcgaagaa tctgtcaacg aaaatcaagc gagcgggcga ttacgggagg
     5281 agaaaattga cgccgatcat cctggacggc cgatccgcgt ggagataccg gtcctcgaag
     5341 ccaagcggcc cggcggcagg ctacgaccgg gcaggttccg gaagggccgc cctacttccc
     5401 taccccctcc cagcaggcgt ccgccagctc ctggaacctc aggtgggctc cgggtatgtc
     5461 ctggaagcgg ttgcaggtga aggcgaacac gaggcccgag ggggggtcgg cccagccgaa
     5521 ggcacagccc ccctggccgg agtggccgaa ggtccgctcg gtggctcccc tgcccatgtg
     5581 gcgaatgtcc cggtccggga agggcgagta gccccccagg ccgaagccca gggcccagcg
     5641 gatccgcatt ccggactggg cgtccgggcc gtcccagcgc aggaccgtgg cgcgctccac
     5701 cgcggccttc gaaagatagc gccgcccgtc cagctgtcca ccggcgcaca gcatccggta
     5761 gaaccgggcc aggtcccggg cgcaggtgtt cagggaggcc gcgggcagga aaaggcggcg
     5821 gtacaggggg ttcgagaaga cccgggcggc gccgatctgg gcgggatccg cggtgtggat
     5881 gcccgatgcc cggcggagct cccccggggg gaggcccgga aaactgtccg tcatgcccag
     5941 gggcgcaagg aactcccgct tcaggtagtc cgccgcggaa aggccggata cccggcggat
     6001 cacctcccca aggacgaagc ctcccgtgaa ggcgtggtag atgaccttct cccccggggc
     6061 gtgctggggc ctccgggccg cggccctgcg ctctcccccg cccgggacca gccaggacag
     6121 gagctccgga aggtccgcgg cggcgggtat gcccgcctgg tggaggagga cgtgggcgat
     6181 cgtgaccccg cccttgccct ttgcggcgaa ggcgggccag tactcggcca ccggggcgtc
     6241 caggtccagg cggccctcgt ccgccagctt gtgcacgcag gccgcggtca gggccttggt
     6301 ggccgaatag gccgcgaagg gggtgtcgcc gaggacgggc cgggctcccc ggggatcccg
     6361 gaacccgccc caccgggaca ggaccctgcg gtccccgtgc cagacctcga tctgggcccc
     6421 gaaatggagg ccccgcagga tctggccgtc gaaggcccgg gccgccccct cgaaacccgg
     6481 ggcggtgacg aagcccggac cggcggcctc ggcggcgctc acccccggcc tcccgaaacc
     6541 ttgacctccc cggtgcggac ccccgagatg cagaggaagg agagcacgaa gaagatcggg
     6601 cagaccagga agatcaccga gtagtcccgg cccgccaggt cgatgacccg gccgttcagg
     6661 atcggcccga tcgtcccggc cagggtggtg gcgatgaagt agaggcccga gtaggtgccc
     6721 atcagttcct cggaggtgga ggtgtccagg atcatgggaa ggcagttcac gttgaccagg
     6781 gcccagccga atcctccgac cagaagcatg gccgccacga cgggcaggga ggggaagagc
     6841 cggatgaccg ccagccccgc ggcgaaggtg aggagcccca cggagatcgt gatcctgcgg
     6901 cccaggcgct tggccacgag gctggcgggc agggcgaaga tcaggaagga gacgtaggac
     6961 accgccaaca gcatggccgc cgccgagggg gccaggccca gacgggtgac cgcgaaggag
     7021 gaaaagaagg tctcgatggc catgtagccc accatgtaaa agaagaccgt caggatcagg
     7081 aggaccaggc tccgcttgtt ctcccccggg atctcccgga gccgggccag gacccgcaag
     7141 cccccgggag cctccgcggc ctgggccagc tccgggggct cccggacgct ccggaagagc
     7201 accaggaccg cggccaccgt agccagggct ccgaaccaga acggcagggg ctggtagacc
     7261 gcgaagaggg cccccccgac cacgaaggcc aggatgcccc cgatcccgga catgatgttg
     7321 accaccgcgt tggcctggga gcgcagggcc gagggaaaca ggtccggcag gagggcgaac
     7381 atgggggtcc gccagagggc catgcacacg accatgacca gggccgcgcc catcaggagg
     7441 gcgaagggtc cccccaactc ggataccctg cccgacgatt ccgggggtat gagctgggtg
     7501 cccatgggaa ttagggcgaa ggccagggcc gagagggggg cgaagaccag gatccagggc
     7561 atgcggcggc cgaagcggga acgggtccgg tcgctcaagg gtcccatgat cggctgtagg
     7621 aagaaagcgg cgacgttgtc cagggtcatc aggatcccgg tcaccgttgc cgagagcccg
     7681 aagccccgca ccgcggcggc agcccggaag cccgggcttc cggcctggag gaagatcggc
     7741 atgtaggtgt tgtagaccat ccacatgagc tgggccccga agaggccgaa gcccaggagg
     7801 gcggacttcc tgtagaactc gggggtcagg gcggattcct tcggggacat cggagcctcc
     7861 ggttttttcg atcccgccgg aaccgggtcc ggcgggcccg gaggacaagt accccgggcg
     7921 gggtcattgt cgcccccgcc gaccgcgccg tcaaggcgag ccggacaatc acttccttcg
     7981 tccgaagagg cgcccgagat cccgcatcag ggagcccgcg cgccgggccg tcccgtggcc
     8041 gttggcgaag gcctcgaccg actcgtccaa cccagagacc agggccacgt ccccggggcg
     8101 gaaccggtac tccggggaga acaggccgta ctcctcatcc ccgttccgaa ccgagatcag
     8161 gttgatcccg tgattcttgc ggaggttggc ctccagcagg gactggccga agaggcgatc
     8221 cggcaccacc agctccgcga tgaccaggtt gcccgagacg ggcaggtagt tgaggagtcc
     8281 cgaggacacc agcagggggg tgacccgctt ggccgcctcc cggttcggga agacgatctt
     8341 ggtggctccg accatctcca ggatctccgc gtgcccctcg gtgacggcct tgacgaagat
     8401 ccggggaatg cccagcttgc ggcagtagct ggtggccagg atggaggcct cgatcttgtc
     8461 ccccatgtcg atcaccaccc cgtccagccc ctggggcagg gccttcctca ggttctcctc
     8521 gttcacgatg tccatgacga tggctgtcac cggataatcc cggtaggcgt cgatgacctc
     8581 ccggtccttg tcgatgatca tgacgtccgc gcccatgtcc aggagttcca cgagaacgtt
     8641 ctttccgaac cagctcaagc ccaaaatcgc gaattgcttc atatccctac cctatgagta
     8701 tctccgcctc gggatacacg aagttgcccg cccgggtcga accgcccagg aacaggaagg
     8761 ccagcagtcc caccctgccg gtgaacatgg tgccgatgat cacgagcttt cccgccgtgc
     8821 tcaagcccgc ggtgaagtcc agggacaggc cgacggtccc gaaggctgag gtgacctcga
     8881 aggcgatgtg gccgaagtcc acaccccggg gtgcctccac cacgctcagg attcctgccg
     8941 cgaagatgag caggaaggcg gccttgatga agtagatcac cgccgaattg atcgtctccg
     9001 gcgtcaaccg tctgcggaag gaattgatct cgcctctctc gttcgcccgt ttcagcatga
     9061 ccaggaggac cacgtaggcg gtcgcgatct tgacgccccc cgcgatggag ccgggcgcgc
     9121 cgccgatgaa catcagcagg atcgtgatga acttggaggg ctggcgcagg cccgcctggt
     9181 gaaccgcgtt gaagcccgcg gtgcgggggg tcacggactg gaagagggcg ttcatggccc
     9241 ggaccggtcc ggacatcccc gcgaaggcat ggtccttttc caggatccag aaggctccgg
     9301 ccccggccag gatcaggatg gcggtgatcg atagcatgag cttggagtgg tagctcagga
     9361 tccgcttctt gccgcggatc ctgcgggcga tatcctgcag aacgatgaag ccgatgcccc
     9421 ccgtcgcgat gaggaccgag aaggtcccca ggagaacggg ctctcccttg aaccgctcca
     9481 ggctgtccgg gaacacggaa aaccccgcgt tgcagaaggc ggatatcgcg tggaaaattc
     9541 ccaggtagat cgggtccgcc tggcccaggc gcgcgaactg ggccgccagc gccacggctc
     9601 ccaggacctc gaccaggaag gtggaaacca cgatgctccg caggatccgg aaggggtcgt
     9661 tttccacccc ctggatggaa aagctccgga tggtccggag cctgcggaag ggcagccgcc
     9721 ccccgggaag gatcagcatg aaggacgtga aggatacgat ccccagtccc cccacctgga
     9781 tgagccccag gatgacgagt tggccgaacc gggaatagtc cgcggtgtcc acggtcgtga
     9841 ggcccgtgac acagaccgcg gaggtcgagg tgaacagggc gtcgatcggg gacaaggata
     9901 ccggcctccc cggtcgtccc gcccaggcgg cgggaagcat caacaggaag gtccctacga
     9961 agatcacgat caggaagaaa gacaggagat aggcggcgtc ggacttgaat gcccgtttca
    10021 tgtcagcttt ccagcttcag gcgatacccg acgccgggtt cggtcaggag ggtccggggc
    10081 acggagggct cggcctcgat cttcttgcgc agctggttga cgtagactcg gaggctgttg
    10141 tagtccccgg ccatgtcggg cccccatacc tcccggagga tctggttccg ggtcaggatc
    10201 ttcccggaat tccggaccag catctccagg atggaccatt cggtcggggt caggtgctcc
    10261 tcggccccgt cccggaaggc ttcccgggtg tcgaaccgca cctccagaag cccgacccgc
    10321 aggggccggc cctccggaga cccgagtccc cggtggcgga ccgccacccg gatccgcgcc
    10381 atgagctccc ccaccccgaa gggcttgatc aggtagtcgt ccgcccccgc gtccaggaga
    10441 cccgtgatct cctcctcgga atcccgcacg gacaggacga tgacggggac atccccccgc
    10501 ctgcggacgg ccaggaggag gtcccggccg tccccgtccg gaaggcccag atccaggagg
    10561 acgacgcccg gcttgaccgc gccgaactgc ctcaggccct cctccaggcc gtccgcctcg
    10621 aagacctcgt agccctcctt ttccaggtag agccggagga gcttccgtat ctggacctcg
    10681 tcgtccacca cgaggacggg aatcacgatt cctcttttcg gggcagccgc agctctatca
    10741 tgaaacctcc ccggtcgctc cgggcggccc gggcggagcc cccgtgggcc tccacgatgc
    10801 cccggcagat agacagcccc agcccgcagc ccgggcgggc cccggtccgg gatccccgga
    10861 agaaggtatc gaacaggagg gggagttccc gttcggatac cccgggaccg tcgtccgaga
    10921 agcggacgac cgtggccccc ggcaccgatt cggacccgac gcggagggtg gagcccgggg
    10981 gagtgtaggc cgagaaattc cgcaggatgt tccggaaaac ctggaccatc aacacagggt
    11041 ccgcggtgat ctccgcatcc cgggcggatt cctccagggt cacggttctc ccgcccaggt
    11101 cggattccag accctcccgg gcggctccga tcagctcccc tacgtatgcg gtctccgggc
    11161 ggacgaccag ccgccccgcc tccaggcggc tcatggccag caggtcctcc acgatccggt
    11221 tgagcttgcc cgcggcgatc atcgtctccg aaagaagctc ccctctcagg gccgggtcct
    11281 ccgcggcccc ggcctggagg gcgctcacgg aacccgtgat cgtcgtgagg ggcgtccgca
    11341 gttcgtggga gacgtgattc aggaggatcc tggaaagccg ggcggactcc ccggccagct
    11401 tgttccgctc gttctcctcc gccagaagct cccgctccag ggccagggcc aggttgccgg
    11461 ccagggccga caggacttcc cggttctctc cgtccagggc cctctccccc ggtcccgccg
    11521 cgtagaggat tcccaggacc ccgtcctggg atcccagggg gatgtacaac ctcccgggat
    11581 cgtccgcgac gcagacatcg gactggaaac accgggccgc ggcgctcgga tcgaagcccg
    11641 gcgccgtcgg aggcccgccc cgcacgggac ctccggtgtc gagggtcccg tcggtgccgc
    11701 ggaggaagac cgaggcacgc accctcatat gccgttccag gtagtccgac gccaaccggg
    11761 ccaacccggc gatatcccgt tcccgggaga ccgcccgggt aaagccgtac aggaaggcca
    11821 tcctgcgttc ccgcagggac agggcggact ccttctcctt tagccgggag gtgagcaggc
    11881 ctcccgcgaa ggccgccagg aagtacgccg tgaacatgag caggtcttcc aggctggcga
    11941 tcgagaaggt caggcgggga ggtatgaaca ggaagttcca caggagcgag gatagggccg
    12001 cgctggccag gaccgtgatg cggccgcatg cgaacggcag ggtcatgttg accaggaggt
    12061 acaggatgga gacggacctg taacccaggg cgggctgggc caggtgcccc agcagggtga
    12121 tcgaagccag ggcggccagg gccaggggga ctccccgcag cggtccggag gaatctcgga
    12181 tcttcagggg cctcctcacg gacggaaccg gggcctttcc ccgaaggatc accacgtcca
    12241 ggtccccgga ctcccggagt atgtcctcca tgacgctgcg ccggcccagg aaggatcggt
    12301 cggcctcctc ggccttgccg atgaccaggg tgccggcgtc cttgacccgg gcgtggcgga
    12361 cgatgcagga agccacgtcc cggtccacgg cggagagtat ttcggctccc agcgtccgag
    12421 ccagggccag gttccgttcc acccccgcgg gatcttcgtc gcattccggg tcgtgcacgt
    12481 gcaggaccgt ccaggcggct ccctgccggc gggcggcggc gtcggtccac cggatcaggt
    12541 actcggagtt gcgactcccg gtgacggcga cgaggaagtg gtctccgagc ccggactcgg
    12601 aacggctcga aatcggttct tccatgcccc gagcatagca cttcctaaaa acctcgtccc
    12661 attaaggaga cgttaagaaa ccctggagtt tcgttaagga tcgattaaaa ccggaggaac
    12721 cgggctatcg gacgaggacc acctgcaggt cgcagacgtt cgtgttggtg ggcccggtgc
    12781 gtaaaaggcc gccgatccgg tcgaagaagg gataggagtc gttggccgcc aggtagtccc
    12841 ggggcttgag ccccgcgcct cgggcggctt ccaggagacc tgtatccgcg aaggcccccg
    12901 cggcgtccgt cggcccgtcg tttccgtccg tgcccgccga gaggaagcag acccgctggg
    12961 cgagccgtcc ctccgcgtcg gaagggcccg ccaggaggga cagggccatc tcctggttgc
    13021 gtccccccgt gccggtcccc cgcagggtga ccgtggtctc ccccccggcg atgaggcagg
    13081 cgggcctgct ccggggcgtc ccgtcggcgg cgcagtccag ggccatgccc aggtaggccc
    13141 gggcgatctc ccgggcttcc ccggtgatcc gggaccccag gtagagggta cggtacccga
    13201 gctgacgggc ccgggccgcc gcggccatgg ccgcccggcg gttcgagccc agcacgatgt
    13261 tggcgaccga ccggaagatc ccgtccccgg gcttgggggt gtccggcagg aggccggccg
    13321 ccccgtcccg gagcaggtcg gccgcggcgg cggggatctt cttctcgatc ccgaagcgcc
    13381 ggatgatccc cagggcgtcg gcccaggtgc tcgggtcggg gaccgtcggc cccgaggcga
    13441 tggaggccag ctcgtccccc acgacgtcgg agaggatgag ggacacgacg gaggccgggg
    13501 ccaggatttc cgccaggcgc ccgcccttga ccgcggacag gtgacggcgg accgcgttga
    13561 tctcccggat gtccgccccg caggccagga gctcccgggt gaccgcggcc ttgtccgcca
    13621 gggagagggt ccggccgtcc cggtcgtagg gggcgcagag gatggcggat ccaccgcccg
    13681 agacgagcac caggaccagg gtccgctcgt ccgccccgcg ggcggcctcc atcacggccc
    13741 gggcggcccg tacggaggtt tcatccggca cgggatgggc ggcctccagg atgcgcaccc
    13801 gatcgcaggg ctccgcgtgg cccgccttga cggccaccac cccgccggac aggcgcttcc
    13861 ccaggacctc ctcggccccc cgggccatcc gggctccggc ctttccgtag cccaggacca
    13921 ggatccggtc gaaacggccg aggtcccagg agaggggacg gccgcctgcg gccagcgtca
    13981 ggcgaccttc ctccggtttc agcgcgtccc ggaccatagg ccggggatcc acgctggcca
    14041 cgcccgcggc gaagatatca gtcgcatcct tcctcaggcg ggcgtggtcc acggcgggtt
    14101 ccttacacga acagggacat cacggcgacg acgagagccg ccacgacacc ttccaccgcg
    14161 gtggcgctgg aataagcctt gtaggcggtg ttcacggaca tcccggagaa ctgggagacc
    14221 acccagaagt aggagtcgtt ggcgtgggac accaccatgg caccggcccc gatggccacg
    14281 accgccaggg cgggatccag gccgatgctg gccatcatgg gggccaggat cgtggaggtg
    14341 gttatgatgg ccaccgtgga ggacccctgg gcggtcttga gggccgcggc gatgatgaag
    14401 ggcaggaaga ccccgaggga caggctggtg aggttcccct tgaggaattc cacgatgggg
    14461 ctggcggcga tcatggcgcc gaaggagccg cccgccgcgg tgatgaccag gatgatggcg
    14521 gagtccttga tcccctggcc cagccaaccg tccacggcct tgccgagctc gcccttcttg
    14581 accaggagca ggctgcagaa gacgccgatc atgagggccg tgacggggtc gccgatgaag
    14641 cggatgagct tctgcaggga ctggtccccg aagggcttgg aggggaattc cgcaacgctc
    14701 ttcaggagaa tgagaacgat gggaagcacg atgggcagga aggagaggaa ggtgctgggg
    14761 agtttgccgt atttcttcac gatgtcgctg taggactcct cgacatccgg ctcgatctgg
    14821 tacttcttgg ccaccttggt cgcccagaac agacccgcaa gggacgcggg caccgcggcg
    14881 atgaggccga agaggatcac ccggcccagg tccgcggaga gggcgcccgc cgcggcgatg
    14941 ggacccgggg tggggggcac catggtgtgg gtggcgtaga ggcccgtggc cagggccacg
    15001 gccatgaccg ccatggaagt tccggtctcc ttggccaggg ccttgttcag ggggttcagg
    15061 atgacgtagc cggagtcgca gaacaccggg atggacacca cgtagcccgc ggcgttcatg
    15121 gccagggggg cgcggttctt gcccaccagc ttcaggatgg cctgggtcat ggacagggcc
    15181 gccccggtct tctccaggat cgtgcccatg atggtgccgg ccacgatgac gatgccgatg
    15241 ttcgtcaggg tgccgccgaa cccggtcttg atcttgccca ccacgtccag ggcgggcagt
    15301 ccgatggcca ggccgaaggc gaaggccacg ccgatcagga cgaggaaggc gttcaccttg
    15361 tatttccccg tcaggaatac gatggccacc accgcgacca ccagcaaaag cagagctacc
    15421 gttccggata ccatgggctc ctccttgggt aaggcgagaa tggatgattc taagaccgga
    15481 atccggcttt ggtcactggg aactgcgcat ccgtttcaaa gatgctcctg tgcatcttgc
    15541 acagtaccgc cccgcaattt cagataggcc gccatggacc gggagaggga cagtgcgccg
    15601 gcgtcggccc gggggtcgca acccaggaag gaggacaacc tcgcgagccg ggcggagaga
    15661 gtgttgcggt ggacacccag ccgggccgcg gcctccttcg cgttgaatcc cgaggcttcc
    15721 agggctttta aggtgggggc cagggtccgt cggagctccg cgggcgcccg ggccgcgatc
    15781 gaggccaggg cgtcgtccag gacgatccgg ggagtccggg ccaggaaata gtcgtccagg
    15841 tggtccagga tcaggaccgg cccgtccccg ggggggacgg ggaaccggga ggccagccac
    15901 agggcctggc ggtagctccc gcgataccgg gagaagtcgc cttgggggga accggcaaag
    15961 cacgtcacct tggggatgcc ggccgcccgg gagaaggccc cgcagtacgc ctggacctcc
    16021 ccgcggtagg cggccaggat gcccgggacc cgctcctccg gagcctcgac cctctcggtg
    16081 cccagcctgc ggaacaccag gagggacccg tctggcaggg cgctggctat atcctgggcg
    16141 tcatgggcgc cccgggcctt ggcctgggcc agggcatcct cgggcccctt gcccgggggc
    16201 agcctgagga ggagcgctgc gcgttcgatc gccgggtcgt agcccagctt gccggccagg
    16261 gccagcacgg cggattcggg gacggcttcc tcgtacagga ggtaggccat cagggaggac
    16321 ttgcgggtcc tgcggttcag gaccgcctcc cgcatggact ctagctcgat catggactcc
    16381 acgctggtct tgaccgcctg ggccaggtcc cccacgacct ggggatcccc ggtcaccccg
    16441 accaccccca gcacctcccc ccggacttcc aggggcaggt tcaccccggg cttcgtgccc
    16501 tcgggcaggt ggtccccggg ggagatcctc tcctcccggc ttccggattc caggaggtga
    16561 cgggccgctg cgtgataggt tccgacccgg gccgggtccc ggctggctat gatgaccccc
    16621 tcccggtcca tgatgttgat gttgtagtcc aggcgctccc cgagcttgcg caggaaggcg
    16681 ccggccgtct ccgcgtccag gcggagccgc tcccccgggg ttgccggatt ggaggtaccg
    16741 cccgaggctt cccgatcagg ggacatcccg gacattcctt cccggctgga accccggcgc
    16801 ccggtaccgg ccgaacctct gcgtgaagcc ccgggagaag agggagccga aggccccgct
    16861 tcgttccagg gcgcgcagga ccgcgtccag gggtcccgcc tgaaggaggc tgaacccgag
    16921 atagagggcc aggccggccg cgaaagcgcc gatcccgccc gaccgcccgc cccgcccgga
    16981 tgcggtttcc aggagggcgc gggccgcggg gcccgacagg aagagggccg cgacgttcag
    17041 acccagcacc aggaccagtc gggcgtagga ggtctggatg gcccgctcgg ggcagaggtt
    17101 gatgcagcgg ttgcagccgg agcacgccgt ggtccaggcg ggccgggctc cggtcatgcg
    17161 gatcgccgcg gacgggcagg tccgggcgca gagcccgcag gaggtgcagg atccgtccgc
    17221 gacgaagagc cgggccagga ggcggcggcc gaaggtccgg aaccctcggg ccaggagggg
    17281 gccgagcgcg cgggagacgg gtccgcgccg taccgagccg gccgggagcc cgggagagtc
    17341 cgttccgccg ccggccaggg cggcggccag gcggacgccg aactcccggg cttgcccgtc
    17401 acccaggctc gtcatggcag cccgggccgc ctcatcctcg gggggcgaaa cttgggtcca
    17461 gttttccgga taactggcct ctaccgatcc gaccggaacg gcgcctttcc ggaccaggat
    17521 ccggagggct tccggaaccg cggtcccgct ccagccgttc acgacccgac ccttcgcggc
    17581 ggtcgccccg caggcgcaga ggacggcggc ccgaagtccg gggagcccgg gcagggagcg
    17641 aagggcccgg aggacgggcc ggggcgcgga aaagcccagg gccgggaacc ccagcaccag
    17701 aaggtcgttc tcccgaaggc actgcagggc ggaacccagg gacggtcctc cggcgcggat
    17761 ctcgagccac tccgcctccc accccgaggc ctctagcccc tcggcgacct tctccagcgc
    17821 cctcagggtg ttcccggtcc ccgtgtagta cgcgaacaac gccttcatcc gccccttcct
    17881 ccgatcggca ctcgccgcgc acagagtacc acgggggccg ggatttgtcc ggaatcacgc
    17941 ccttgactta cgcggggtgc cggagtatgc cgatacgggt atccgaagga gccgccatgc
    18001 acccacgccc caagctcttc gtttcccgct gtctcgggtt cgaggcctgc cgctacaacg
    18061 gcgcggtcat cgaggatccc ctggtcgcca agctggccgg cttttgcgac gtcacgacgg
    18121 cctgccccga aaaggacacg ggcctggggg ttccccggga tcccgtacgc ctggcccgtg
    18181 cgggaggcgg gattcggatg atccagcccg cctcgggccg ggaccatacg gacgccatga
    18241 cggccttctg cgcggccaag gccgcggagc tggccgggac ggcccccgga tcggcccgcc
    18301 cgcccgaggg ccttcccgcc gtggacggtg cggtgctcaa gtcccggtca ccctcctgcg
    18361 gaatgaagga cgtgaaggtc taccgctcca cggagccggg agccatggcg gacgtcggga
    18421 tcgggctttt cgggcaagcg gtcctgtccg ccctgggcgg ggcccccgtg gaggacgagg
    18481 gccgcctcaa gaactacacg atccgggaac atttcctgtc cggcctcttc gcccgggccc
    18541 ggttccgcgc cgtcgccgag tccggaggtc cccgggagct ctcggagttc caggcccggc
    18601 acaaactcct cttcctggcg gtcaatcagg cgtccatgcg cagactcggc cggatcgcgg
    18661 ccaacctgga aagacacccc ttcccggcgg tcctgtcgga gtacggggcg gaactgggca
    18721 cgatgttcgg gagcccggcc aagccggggg gcgtcatcaa cacggccctg cacgccttcg
    18781 gcggcttctc ggagcgcctg tcgccgttgg aacggaagta cttcctggac gccgtcgagg
    18841 agtaccggga cgagcgcatc cccgcctccg ccctcttcac cctgctcaag gcctgggccg
    18901 tccgcttcga ggtgcgctac ctcctggacc aggtcttcct ggaccccttc cccaaggaac
    18961 tggtggaaat cacggattcc gggaagggtc gggaccgata ggtcgccgat ccggacggag
    19021 cggacgccag gcgttggccg cgcgcctccc tcaggccccg ggttcccaga gaaaagcgga
    19081 ggccttcttt cccagcacgg tccaggacca ggaccgcgat gccggccggc cgccgaaccc
    19141 ggcgcagacg tggaggggca ggaggtgctc ctcccgggga tggcagtacc gggcgtgggg
    19201 ggcccgctcc cactccgcca gccttcgatc ccgctcgccg gccggaaggt ccggggaggc
    19261 gcaggtttcc gtgagccagg cctggaaggc ttcgtttcgg ggatcgggac tccccggggc
    19321 ggccgagaag aactccctca tgttgtggaa agaaaacccc gaacccagga tcaagaggtc
    19381 ctccccttgc agggccgtca gggcccggcc caggtcgagg tgggcgcggg gttccaggcc
    19441 gcggatcagg gagacctgga cgcacgggac gtccgccccg gggtacatca gggccagggg
    19501 cacgaagacc ccgtggtcga atccgcgtgc ctcctcgggc cgggcgggga tccccgcggc
    19561 ggacagggtt tccaggatcc ggccggcaag ggcgggatcc cccggggcgg gataccggat
    19621 ccggtaggct tcctcgggga agccgtaata gtcgtagagg agctccggtg cgggcccgcc
    19681 gatcacccgg acctcgggct cttcccagtg ggcgctgacc acgaggatcg cggacggacg
    19741 gccgagatcc cggccgagtc cccggagacc cccgaccagc tccgcgtggc cgggatcgcc
    19801 caggaggggc agggggccgc ctccgtgggg aatgtacagg gcgcgggctc gaggcatgct
    19861 catggggacc tccttgatcg aagctcggcc cggacgccgt tcctcaatat cccgtggatt
    19921 ctcgcaccaa acgatgagat gatctatact gacccgagga gtcagcatat gaaacctctc
    19981 gtcctgctaa gaaccctcgt ccggaccgcc ggcatcctgg ccggggcatt cctggtgttc
    20041 acgggtctcg cgaacctgcc ccggatgttc ctgggcatcg gcgtcatcct agccggcctt
    20101 ggcctcgggg ctctcgtgaa cgcggctctc cggaaagccg ggcagtacat cccgcccgag
    20161 atcgtcgcgg atcccggaat ccgaaacccc gtcctccgct ttctgatcca gccggaaatt
    20221 ctcatcgtgg tcttcggctt cttcttcaat ttcttctggg aggtttccca gatccccctg
    20281 tacgagggga tcaaccgggg catcccctac ttcggggaga acacggtgga gatgaagctc
    20341 ttcttcgtcc ggaccttctg gcgggccgcc ttcctcgacg ccctgctcct ggtcggtgcc
    20401 cacctcctga ccctggcgat cctgagggaa cgattctggt tctccaaggg agggcggctc
    20461 ttcggtatcc gttcccttac cgtccgcccc tgggtcggct tcgggatctc caccctcatc
    20521 tgcgtggcct tcctgatcta cctggagatc agcgccttcg atcagggcct ctgggcctac
    20581 tcccgcctga tgccgacctt gttcacgaaa atcggcctga cgcccgtagg aggcctcctg
    20641 gtcagtccga ccctggtgtt cctgctgacc cggagggtaa gtcgaccggg gccgccgctg
    20701 tcttgacgga gatcgagatc cgggacggac ggccggagga cgcgcccgtg gccgcccttt
    20761 tgatccggga agccggggac cggctcatgg acttcctggc gggattccgg ggccgggcgg
    20821 cgacggaaga ggttcttgaa gggctctgga atctcccggg tacccgcttc agccgggagg
    20881 ccgcgcgggt tgccctgacg gacggcgagc cggcgggcat cctggtcggg tatccccggg
    20941 aggcgatggc gggcctggac ctcgggacgg gacgggccct gctgggcctg cggggtctcg
    21001 ccctggcggc gggggtcctc cggcatcccg tgcttacctg gcgcatgtcc accctgcccg
    21061 aggcggaacc gggggactgg tacatctgcg tcctggcgac ccgggaagcc tgccgcggca
    21121 gaggcatcgg ggcggccctg ctctcggacg ccgaatcccg ggcccggcgg cacggcgcag
    21181 cggccctctc cctgatggtc tccgagggca acgccccggc ccgatccctg tacgtgaagt
    21241 tcgggttcga ggaacggggc cgcttcaccg tggcgggccg gacgagcctg aggatgggga
    21301 aggccctgga aatgctagag ccgcggtaag tattcccgac ctcatgagct tcgctgtatc
    21361 acaggattcg ttggattatg cgtatcgtga cgccttcctc atccctcttt gccggaattc
    21421 ttaaaaatcc cctcgaacag gaagtccacg taggctccgg ccaggtcccc gggggaaagg
    21481 ccccgggaat cctcgagcca ggaagccgcg aggcccagga tcatcatgga cagggcccgg
    21541 gcggccaggg gttccggaag ggagaggcgg atcccggaga ggcgggcgag tcgggattct
    21601 cggaggtagg ctgagaaata ctcttcgata cccaggaaaa cccgggaacc cgcgcggccc
    21661 gaaagcaggg gacggaagag ggaggcattg tcctgcaaga cccgaaagaa tctctccacc
    21721 cgggcccgga tccgctcccg gggacccgaa tcatccgccg gacgggaacc cgtacgagcc
    21781 cgtccgacgg actccggatc gatctcggcc agcagttccc ccaggtagag acccaacccc
    21841 cgctccgcaa ggtcctcctt gtcctcgaag tgccggtaga aggtggcccg gttgacctcg
    21901 gcccgttccg cgatggaggc cacggtggaa gcggggccgc ctccttcaga gcagaggtcc
    21961 aggtatgcct ggactatggc tcgccgggtc ttccggatcc tgggatcctc ttcgcgaccg
    22021 ttctcagcca tggtacctcc cgcttcgcgg acgggattca gcataggatc ccgggtgcgt
    22081 cggatcaagc gcttatgtcg agtacgcgac gctttcccgg gtccgtgtcg cgggggcgga
    22141 tcctggaaac cgggacacgg ccgaaggcgg ccgatatcct gtctttgcgg aacgggccgg
    22201 aacagcgggc ctcgccgcgc aggagacaac catggatgaa agaaggaccc tgaagatcgg
    22261 actcgtgcag aactccccga agccgggagc cgcgatcgag aacctcatgg acgcccgtcc
    22321 cctgatcgag gcggccgcgg cggcgggggc cgacctcatc gccttgccgg aactctcggc
    22381 ctcgggttat gccccgaccc ggggcatctg gcgcagcgcg gaacccgcgg atggacccac
    22441 ggagcggtgg ctcggggaga cggccgatgc cctgggagta tacctctgcg cggggtacct
    22501 ggagacggac ggaacggatt tcttcaatac ctacgcggtg gccggtccgg agggaagact
    22561 cctcgggaga gtccggaagg agaccccatc catgggggaa acctacctct tccagggaga
    22621 gaccggttct cacgtcgtgg agactcccat cggcaggatc gggatcggga tctgcaacga
    22681 caaccacatg gcctacctgc caggccgcct ccgggccggc ggggcggaac tgctgctcat
    22741 gccccacgcg tggcctctgc ccacggagcc gtccagactg gtctcccggg aggacatcga
    22801 gcgccagagg gcgaacatga cggagctgcc cgcccggtac gcccgggggt tgggaatccc
    22861 cgcggctttc acgaacctgt cgggcaccct ggacttggga ggggaacggt cgccgggatt
    22921 cctgggagcc ctgcttccgc ccccggagaa gtacgggttc ccgggatacg cggcgatcca
    22981 cgccgcgggc ggggagatcc tccggtcccg ggcggacggc gagggctgga tcgtcgggga
    23041 ggtcgtcctg gggaccggca tgcggcccga ggcttcgatg cccgcgtacg gacggagagt
    23101 gtatccgggt aactcggacg gcgggatcct ggcgctctac gagagcctgg gccgccgatc
    23161 ctacgcgcgt tcccgggatc ggcggacagc cgcccgggcg gccttcgagg cgggccggac
    23221 gagcctgagg atggggaagg ccctggaaat gctagagccg cggtaggcat tcccgccctc
    23281 atgagcttgg cgatatcaca ggattcgtca aatccttgat tgtccgcccc gccgccgcgg
    23341 ggtataatgc ctcccatgaa tcccacccga tcccgagcgc tccttgccgt cgtcctggcg
    23401 gtgctggcga tcgcggcggc ggccgcccag gcccggccgt ccgggaccgc cccggccctc
    23461 ccctatcccc ggacttcgtc ggagtgggcg gccttcctgg ctccggaccc gcgcttcgtc
    23521 gagctcggcg gcatccccga gcccatgccc acccggaagc tggcggaggc gggcctcgtc
    23581 gcatccggcg ttccgccgga gcggatggag tactacatag gtaaactgcg ggagaccttc
    23641 gaggccctgc gcgcccggtc ggagtacatc caggatcccg ccctgcgggc cgaatcgatc
    23701 cttcccttcc tgcacaggac cctgttcaag aagtacgagg agaactcgac aaccgtggac
    23761 ggggttatcg acacggggta cttcaactgc gtctcctccg cggtggtcta cctcctggcg
    23821 gcccgggtct tcgtcctgcc catgaagggg gtccagaccc cggaccacgc cttctgcgtg
    23881 atcaccgtga acggccggga catcgacgtg gagaccacga accccttcgg cttcgatccg
    23941 gggaccagca agaaattcca gagcaccttc acgaagacca cgaagttcgc ctacgtgccg
    24001 ccccaggact acgcccgccg caagacgatc acggaaaagg acctggtggg attgatcctg
    24061 cacaaccggg gcgtggacat gcagagacgg ggacgacacc tcgaggccct ccggctggcg
    24121 gtggaccggg cgacgggctt cccgggcccc gacgccgcgg agttcctggc cgcctgcgcc
    24181 cagaacgtcc tggcggagct cttcgaccgc agggactggc cgggcgccct tgatatggcc
    24241 gagggagtcc ggaaactggc ccccgcggat ccgaggcttc gggagctgta caccctggcg
    24301 gcccggaact acgcggtgga cgcccacaac cgcttcgcgg aacgctacaa cgcccgggat
    24361 accgccggtg cgcgggcgat cctggaacag gccctgcgcc gcctgccggg ggatccgatc
    24421 ctcacccagg acctggaagc cctgcgctag gcggcaggcc ggacatcggg acatgggaac
    24481 cctgcgaatc gggacctgct cctggaagta cccctcctgg aagggactgg tctactcgag
    24541 ccgggaaccc gggaacttcc tggcggagta cgcccgcaag tacgacaccg tggaggtgga
    24601 ccagtggttc tggtccctgg gcaggtccgg ggcgggtctg ccccggcggg agacggtcct
    24661 ggagtacgac gcggcggtgc cgccggagtt ccggttcacc gtaaagtgcc ccaacgccct
    24721 gaccctgacc cggccctacg cccggaaggg cgaaacggcc gagccgaacc cccgcttcct
    24781 ggacccgggg tttttccacg gattcctgga cgccctggct cccctggtgc ccaaggtcgg
    24841 actcttcctc ttccagttcg agtacctgaa caaggacagg atccccggcc gggaggcctt
    24901 cctggagcgg ctcgggacct tcctggaatc cctgcccggg gacctgccgt acggcgtgga
    24961 gatccgcaac ccccggtgga tcgatgccgg gtggttcgac ttcctcgcgg agcacggggc
    25021 ggccccggtc ctcctccagg gctactggat ggaggacctg gccgacatcc tggcccggca
    25081 cggcggacgc atccggggcc gggcctgcct gcgcctccac ggggaggacc gggaggggat
    25141 ggaggagcgc accggcgagg attggagccg gatcgtccgg ccgaaggacg gggacctggc
    25201 ccggatcgtc ccggagctgg cgaccctggt ggaagccctg gacgaggtct acctcaacgt
    25261 gaacaaccac tacgagggct ccgcgcccct cacgatcgac aagattcgaa gaagcctgca
    25321 cctttccgag ggggaacagc atggattccg ggcgccctga catccgagtc ctgggccgga
    25381 gggccgcggc cgcgtctctc cgatttctgg catcctccct cgcgaccttc ctggtgatcc
    25441 tgttcatggc cgggttaccc ctgctcatct cggcgaagga cgcccgggtg gagatcgatc
    25501 ccgagcgggt cctggccgcg gcccggggct tcctggcctc cgccctggac ggcaccctcc
    25561 gcaccttcaa gatcggacgg accgcctggg acctccggga gatctacccc aaattcctgt
    25621 ccgtgtcctt cctgtacacg gcgatccccg gagccgcggg gatcctcctg gggacggtgg
    25681 cgggcgccgc ggcccggtcc cggcggcggg gcactttgga ccgtctgacg gacctggccc
    25741 tgggcacgcc ggactttctc ctgatcctgg cggtccagct gggggcggtc tggatcctgg
    25801 acaccctggg gatcgacctg tccctggggg gaaattcccg gctggccctc ctgcccctgt
    25861 ccgtcatggg ggtctacccc ttcttcctgg cccgtcgctg cgccgccgag gcgtcccggg
    25921 aggcggagac ctcggactac atagccttcg cccgggccaa gggcctgccg gaggggtgga
    25981 tcctccgccg ccacctgggc gcggcggtca taccccggct ggaggcggag cttcccgtga
    26041 tcgtctcctt catgcaggga agcctgttca tcaccgagcg ggccttcgcc ctgcccggca
    26101 tggcccgcct gctcttcgat tcggccttct cgggccagcg gaagcatatg atgtggacga
    26161 tctaccagta cgaccatgtc gtgctgtccc tgaccgggct cgcggtttcg ggactgctcg
    26221 tgttcgcggc cctgcgcctg ggcctgggag cggcccggaa ggtgttgacc cgtgaatagg
    26281 cggctggccg cgggcctcct cctgctggcc ctgctcctgg gcctggcgct gttctcggat
    26341 tccatcgcgc cccacgagcg gggcttcatg aaggccgtgc gctacgacac cgtggacggg
    26401 caggaggtca tgctcgtctc cccggagccg cccagccggt tcttcctctt cggcagcgac
    26461 ggctggggct acgacctttt ctcggagatg atccacggcc tgggatggac cctttccatc
    26521 gtattcgcca cggccctcct gcggagcacc gccgccctga cggcggggct ggccctgggg
    26581 gccctccgtc cgacgggcct tcggcgcagg cggttctccc ccctggcctc catcccggga
    26641 ttcatcctgg cggccttcgt cctgtatccg gtcaccatca actcccggct tccgccggtc
    26701 gcgctcttcg cggtccaatc cagcctgctt gctttggtcg agctcccggc cctggcggaa
    26761 tccttcgcgg cccggtcggc ggacgtgctc ggacggccct tcgtggaggc ggcccggacc
    26821 gcgggagcgg gggtgccgtg gatactccgg taccatgtcc tgcccttcgc gttcaccgat
    26881 tggctggaat cccttccggt ccaggccctg tccgtggcgg ccctggtggg caagctgggg
    26941 gttctccgca tcttcgtggg ggggacgatc ctgacggtgg atcccgtgat cttcctgccc
    27001 gccaaggggg aatggctcgg cctgctcgga tactactaca ccggcgtccg cggccagccc
    27061 tggctcttcc tggccccctt cgcggggtgg atgctgatcc tgacctgcgt aggcctgctc
    27121 tcctcgggca ccaaggcggc ctgggcccgg tcgcgccgca tcgaggtccc ggaataggcg
    27181 cgacccgcac cctccgtgca cggtatttgt ttctacgcat agaattcaag cctcttcgga
    27241 acccctgttt cagtagataa aaaagatttg ttgactagtc aaattttgca tgataccctc
    27301 caaacatccc caacatcccc tattctcagg aggcaagtgt gcgtacggtg agatctattc
    27361 tcatggttct gctcgcgctc tcgttggtcg cgatgcccgt cttcgcccag aaggactaca
    27421 agatcggcat catgaccggt accgtttccc agaacgagga agagttccgc aaggcggagg
    27481 cggtccgcaa cgccctcgga gccaaccgcg tcgtcctggc cacgtatcct gaacgcttca
    27541 tgcaggagca ggagacgacg atctccaaca tgatggccat ggccagcgac ccgaaggtca
    27601 aggccatcgt catggtgcag gccgtacccg gagccgccgc cgccatcgac cgcgtgaagg
    27661 ccgtccggcc ggacatcctg ttcatcctgg gcgttcccgc cgaggacccg gacatgatcg
    27721 ccaagaaggc cgacatcgtc ctgcagacga acgacctggc ccggggcgag cagatcgccc
    27781 agcaggccaa gaagctggga gccaagaccc tggtccacta ctccttcccg cgccacatgt
    27841 cctacgagca cctgtccaag cgccgcaccc tcatgaagga agccgccgag aagatcgggc
    27901 tcaagttcgt ggagcaggac gctccggacc ccaccggtga gggcggagtc ccggccaccc
    27961 agcagttcat catggaagac gtgccccgca aggtcgtcca gttcggcaag gatacggcgt
    28021 tcttcgggac caactgttcc atgcaggagc ccctgatccg ccaggtcgtg gcccagaagg
    28081 ccgtctaccc ggtccagtgc tgcccctccc ccttccacgc cctcccgggc gccctgggca
    28141 tcgcggtgcc cccggagaag aaggcggacg tgccctggat cctctccgag gaaaagtcca
    28201 agatcaaggc cgccggcatg aacgggcgca tgtccacctg gccggtcccc gtgaacatga
    28261 tgtacatcga ggccggcgtg aagtacgccc aggcctacgt ggaaggcaag accaagggaa
    28321 aggccaaggt cgatccggcc ctgctgacca agatcatgtc ggagatcgcg ggcggaaagg
    28381 tcgagctcac gaactacaag gactacccga acttcttcat gtacctctcc gacttctaca
    28441 acttctaacc tagggcggca gaaccacccg aaaggcggag tccgttccgg ggctccgcct
    28501 ttttttgaac gccgagtccc aaggaaggag agatcgtgtc gcaaacggaa gaattcgtcc
    28561 ttcggatgaa gggcatttcc aaggaatact acggcaccaa ggtgctcaaa ggcatcgacc
    28621 tggagatccg cccgggcgag gtgcacggga tcgtcgggga gaacggggcg ggcaagagca
    28681 ccctcatgaa catcctattc ggcatgaccg tgatccactc cacggggggc taccagggcg
    28741 agatcgagat cgccggcgcc aaggccgaca tcaagagccc cttcgacgcg atgaagctcg
    28801 gtatcggcat ggtccaccag gagttcatgc tgatacccgg ctacaccatc accgagaaca
    28861 tcaagctgaa ccgggagacc accaaggaca acccggtttc ccgggtcttc ggccccaagc
    28921 tcaagaccct ggacatggcc tccatgggcc gggacgcccg aaaggccctg gaccacctgg
    28981 aaatgggcat cgacgagtgg gcccgggtgg cgggcctgcc cgtggggcac atgcagttca
    29041 tcgagatcgc ccgggagatc gacaagagca acatcaagct cctggtgttc gacgagccca
    29101 cggcggtgct ggcggagtcc gaggcggaca acctcctggc cgcggtccgc aagctctcgg
    29161 cctcggggat cgccatcctc ttcatcaccc accgcctgca cgagatcatg gccgtctgcg
    29221 acgggatcac cgtcctgcgg gacggcagcc tggtcgccca caagcgccgg gaggagacga
    29281 cgatcgagga gctggcccgg ctcatggtcg gccggtcgat cggcgacatg atccggaatc
    29341 ccgccaagtc cctgccggac aggaacgccc tggaaatccg gaacctccgc gtccaaatgc
    29401 ccggcgaggc cgccaagggc gtggacctgg acgtcaagga cggggagatc ctggggatcg
    29461 gcggattggc gggtcagggc aagatcggca tcgccaacgg catcctggga ctctaccccg
    29521 cggaagggga ggtccggttc cacggggagc ccctgcccct gaactccacc cgagaggccc
    29581 tcaagcgggg cctggccttc ctttcggagg accgccgggg cgtgggactc ctcctggaca
    29641 tgagcgtgga gatgaacatc gcggccacgg ccgtggagat ccagggcaag ttcctgcggc
    29701 gcttcgggcc cttctcccaa atcgacggga aggcggtccg cgcccatgcc ctggaaacga
    29761 tcaaggccct ggacatccgc tgcaccggcc ccctgcagcc cgtgcgcagg ctgagcgggg
    29821 gcaaccagca gaaggtctgc atcgccaagg ccctggtcct gggtcccagg ctcctcttcg
    29881 tgtcggagcc gacccggggt atcgacatcg gcgccaaacg gctggtcctg gaaaccctgg
    29941 tggacctcaa ggagcggctg gggatgacca tcgtcatcac ctccagcgaa ctggcggagc
    30001 tccggtcggt ctgcgatcgc atagcgatcg tccacgacgg ccggatcgcg ggcatcctga
    30061 accccgacga cgacgacgcg gaattcggac tcatgatggc aggtgattat ggaaaacgct
    30121 aatgctacgc ccttcgcgcg ggcgctgcgc ggtatcggcg cggggttgcg atccttcggc
    30181 gccccgcgga tcatcatcat cgtcttcctg gtatcgctct ggatcacctc gaccctgctg
    30241 aagatcccgt tccccgcggt cctttcagac tccctggtgc gcctgggaat gaacgccgtg
    30301 ctgaccctgg ccatgctccc ctccattctg tcgggaacga gcctgtactt cgggctgccc
    30361 ctggggatca tctgcggtct cctgggcggg gtcatgagca tcgagctgga cctgcggggc
    30421 ttcgaggcct tcttcggggc cgtggccatg tcccttcccc tggccgtcct ggtcggctgg
    30481 ggctacgggt ggctgctgaa ccgggtcaag ggctcggaga tgaccgtggg aacctacatc
    30541 ggcttcggtt tcgtgtccct catgtccttc ggttgggtca ccctaccctt ccaccatccc
    30601 gagatggcct gggccatagg caagggaatc cgggtgacca tctccctgga gggacggttc
    30661 agcggcgtgc tgaacaacct ttggtccttc gacatcctcg gggtaagggt cccgaccggc
    30721 gcccttgcct tcagcgccct ctgctgcctc ctggtgtggc tcttcttccg gtcccgatcg
    30781 gggctggcga tgaagtccgc cgggatgagc cccaagttcg cggaggcctc ggggatcaac
    30841 gtgaaccgta tgcggatccg ggggaccatc ctgtccacgg tcctgggggc cgtcggcatc
    30901 ctggtctact cccagggctt cggcttcctc cagctctacc aggctcccat gtacatgggc
    30961 ttcatcgccg cggcatccat cctgatcgga ggcgccacgc tgaagacggg ccggatcagc
    31021 cacgtcatca tcggcacctt cctgttccac tccatcctgg tcgtggccct gccggtggcg
    31081 aacaccgtgt ctcagctggg cagcctggcc gagatctccc ggagcgtcgt ctccaacggg
    31141 atcatcctct acgccctggc gcagaaaggg ggagcggaat gagccacgac gcgaatccgc
    31201 acggtacggc cggcacgaag ggagccgtac gccgcttctt cacgaacaac ctggtgaccc
    31261 tggtattcct gttcatcggg atcgcggggc tccgggtctc cgggctcgcc ctgcccttct
    31321 tcatcgtcga catcgtcgac cgggtggcac gcaacgggtt cctggtgctc gccctgatca
    31381 tccccgtgct ggcggggctg ggcttgaact tcgccatcgt cctgggggcc atggcgggac
    31441 aggccaccat catcttcatg acccactggg gcatcggagg aatctggggg atcctgctct
    31501 gcatggcgat atccttcccc atagccctgg cgctgggcgt cctgaccggg cgactcctga
    31561 acaaggcccg gggcaaggag atgatcacca ccctgatcct gggcttcttc gccaacggga
    31621 tctaccagct cgtcttcctg atcctggtgg ggaccctgat ccccatgaag aacgagaccc
    31681 tcgtgctctc ctcgggcgtg ggcatccgga cgaccatcga cctcacggac ggcctcaagt
    31741 tcgggctgga cgaccttctg cgcatccccc tgcctttgtt cgcgatccta tgcggggcgg
    31801 ccgtcctggg atccttcccg gtgcggagcg tcctccggaa acgggccttg gccgcgcggg
    31861 agaagacccc ccttcgggac ggcgtcctgg ccctgacggg cctcttcctg gcgggctggg
    31921 ggatcaagaa cctggtcgtg gaatccatgt acagcttcat ccgcgtgccc gcggggacct
    31981 tcctggtgct gggcgccctt tgcctgttca tcctcttctt cacccgcacc aagctgggcc
    32041 aggacatgcg caccgtggga caggatcgcc acgtggccga ggtctccggg atcgacacgg
    32101 accggatccg catcaccgcc atggtcatgt ccacggtcct ggcggcctgg gggcagatca
    32161 tcttcctcca gaacctgggg accttcagca cctacggcag ccacgagcag gtgggcatgt
    32221 tcgccatcgc ggccctcctg atcggcggcg cctcggtggc caaggccacc atcggccagg
    32281 cggtggtcgg actcttcctc ttccatacga tgttcatcgt gtctcccctg gcgggccggg
    32341 ccctcttcgg ggacgcccag ctgggcgagt acttccgttc cttcgtggcc tacgggatca
    32401 tcggcctgtc cctggccctg cactcctgga agaagcacat gcagcagaag cagggagccg
    32461 ggggatgagg gaggacgtcc ttgcgggagg ccgtcggacg gcggcggcct ccgtctgggg
    32521 ctggctcgtc ccggcggtcg gaatatccga gacttgcgga tttcacgacg gtcttcctca
    32581 gcatactcct ggaggccttt cccttcgtgc tcctgggaac cctgatcagc tcggccatcc
    32641 acatcctcgt gagcgccgag accatcgcgc ggatcctgcc gcgcagccgg ctggcgggac
    32701 tgctagccgc ctcggcggcg ggattcgcgt ttccgatctg cgaatgcgcc aacgtgccgg
    32761 tgacccggag gctcctggcc aaggggctcc cggcacccct cgcggtcacc ttcctcctgg
    32821 cggtggccat cgtgaacccc gtcgtcctat tctccacctg ggtggccttc ggcgggaacc
    32881 tgggggtggt cctcctgcgc ggcctcttgg gcatgggcat cgcggtgctc acgggctgga
    32941 tcgtcggatc cctgccggtc ggccgtcagc ccctccggga caccgccccg ggaggcgccg
    33001 gggaagaaca ggggtgggaa cacggcccgg atcacggccc ggggtgcgac tgcggccatg
    33061 atcatcctca cgatcacgac gccggacacg gggggaaccg cggcgcggac acggggcggg
    33121 ccgggaggcg gggtgccgcg gatgccgccc tcgagatcct gacccatgcg ggtgcggaga
    33181 tctattccgt gggccgctac ctgatcctgg gagccgcggt cgccgcgctg atgcagacga
    33241 tcctgccccg ggcggccctg gtgggcgtgg gagccgctcc cctcctgtcc gtggcagccc
    33301 tcatggtcct ggcctacctg ctgtccctct gctcggaggc ggacgccttc atcgcggcga
    33361 ccttcgcggg atacttcacc cccggggccg tcctggcctt catggtctac ggacccatga
    33421 tggacgtgaa gaacaccctg atgctcctgg acggattcaa gccccggttc gtcgcgatcc
    33481 tcctcgccct ggtgaccgcg gcctgcctgg gggcggggtt cctcgtaaac ctgctcgcgt
    33541 cgggaggtgt cggatgaaaa ggacgcggat ctctccgggt gccgcggcct gggccgccat
    33601 actgctggcc tggtccctgt ggatgctgcg cctgctggtc tcggaggatg tgctcctcta
    33661 cgtccatccc aggatgcggg tcttcgtttg gttcgcggtc ctggggttcc tggtcctgtc
    33721 cgcggtgacg gcccgcagcg ccctgggcgg cacggtcggg cgcgccccga gggcggggta
    33781 cgcgttgttc ctggtgcccc tgacgctcgc cgtcgccgga ggtccgcgcg ccctgggtgc
    33841 cggggccgcg gagaatcgcc tgctgcgagc ggccgcgccc ggaactgccc aatccgccgc
    33901 cccgggccgc acggaaagaa ccctggactc catcctcgcg tccgcggatc ccgggacggt
    33961 cctctccttc gaggacgact tccattcggc catggcggcg gacatggcgg ccgccccgga
    34021 gcgctacgag ggccgcaggc tctccctggt cggcttcgcg caccgggtac cgggcctgcc
    34081 cgcggaccgg gtcttcgtca cccggctcct gatcacctgc tgcgccgcgg acgcggaacc
    34141 ggtgggtgtg ctcgcctcct ggccgggggc agcgggactc cggggcgggg aatggatgcg
    34201 cgtgacgggc accgggaagc ggacggcgta caggaacccc tacacgggca aggattcctt
    34261 cgtggccatg gtggaggtgg acgaggtcgt acccggggaa caacccgcga gcgcctatct
    34321 ctatccggga cagttctgag ggcccgtcag taccgcacct ctacgttcgg gcccgtgatc
    34381 cccgggaagg cccccttgtc cacgaggacc ttgcgctccg ggtgggtaat gatccacttg
    34441 ttccgcatga agaggagacg ggcccgcgga tccccggcgg cggcccgttt ggccgcggcg
    34501 gccttctcct cctccgagag gatctcgttg gtgccccggg gcagaaccac caggctcttg
    34561 aagccccggt cggacagtcg gcagggggcc aggtgcatcg tgcccggatg gatctccagc
    34621 gccgtgcctg cggacaggac caggcacccc acgcggttcg acgggtaggc gggtccaccc
    34681 ggggaatccc agtcgatgtc ctcgaccctg cccaggagca gggccaggtc cgtgacggcc
    34741 acgaggatct ccggggaggc gtgccattcc aggccgttga ggcgggtgtt gggccccgcg
    34801 ttccagccga cctggagggg atagaacccg aagaccggcc cgaaggccgc ggcttccggg
    34861 cgggcttcca ggtccgggtc cgaggcggtg taggcgttgg cctcgaggtt cgacgggatg
    34921 cggggggccg cggcctccgc cagggcggcc aggcccggga gctccgcgat ccggccgtac
    34981 cgggccagcc ggggatcccc ggggccgaag acggctagtc cggggttgag ccgggctaat
    35041 cgctccgcga cggccgggcc ctccatcaga agtccccccg gatcccgccc acgaccagga
    35101 aggtgacggc gatcaccagg gaggccacga cggtcaggac caggcccgcc ttggccatgt
    35161 ccttgatgcc gaagtatccg gcgttgaagg ggatcacgtt ggtcggggtg gaggtcacca
    35221 ggatgaaggc caggctggag gtgagccccg cggccagggt gatggccgat acgttcaggg
    35281 acagggcctt gcccaggccg atgatcaggg ggatgagtat ggtggcggtc acggtgttcg
    35341 aggagaacac caccttcagg aggcagaccc ccagggtcac caggaagagc tgggcgaaga
    35401 ggggtaccga gccgatgcgg cccaggagta tgacggccag ccactccgca gccccggtct
    35461 tgtacaggta ggtccccatg gaaagccccg acacgatgag gaggatcccc gcccagtcca
    35521 cgtccccctc gacctccttc cacttcgtcc gggacatccc gggcaggaag aagaggcagg
    35581 ccgccaggag ggcgggcagg gagatctcga tgaccatccc caggggtttc tggaggaggg
    35641 gggtgaggat ccagatgagg accgtaagga cgaagaccgc caaggtgagc ttctcctccc
    35701 ggttcatggg gggcatgtcc cggagctcct cgcggagctt ctccttggag accttgagct
    35761 tccgcatctc cggggggaag aaggccaaga ggatccccca ggcgggaccc aggatcagga
    35821 gggccgaggg gatcccgtag gccatccagt ccaggaaggt caggtggatg tccgccatgg
    35881 aggccaggaa ctgcatggcc acggggttgg tgccggttcc cgctggggtg gcgatacccc
    35941 cgatcagggc tccccaggct acggcgatca tgagcccctt gccgaagttg ctcttcatgg
    36001 gctcggcccc ctcctcccgg aggatggccc gggccagggg catgagcatg gccgccacgg
    36061 ccatgtcggt gatccacatg gacaggaggg ttcccgcgat caggaatccc agaatgatga
    36121 gccgggtgtc gttgcccgtg acggacagga tgaccgtgga gatccgcttg cccaggccgc
    36181 tcttggtgat gaaggcgccc aacaacagga ccccgatgaa gaacacgacg atgtcgttcc
    36241 cgaagcccgt ggagatgatc tccttgtact tgcctacctt gaggaaggtg aggagcccca
    36301 gggagagcat gcccgttatg tggaagggaa gggcctcggt cacccagagg atcaggcaga
    36361 aggccaggac ccccagggcg gacatggccg cctcgccgcc ctggacggag gcggggtagg
    36421 cgggcttgcc cgtggccgcg tccaccgcct tggcgatggt ctgggccagg gaggccggcg
    36481 ggggcaggac caccagggcc gcgcagaccg cggcggccag ggccaggaag agggcgttgc
    36541 ggaatgtcct atcggcttcg atcttcatgt cgtctcctta tgaatgtgcg gggtcgccca
    36601 ggatttcccg gagcaggtcg cccaccgcgc ggggccggtc ggccaccgcg gccccggccc
    36661 gcttgagggc ggcggccttg ccttccacgg tgcccttgcc gccggtcacg atggccccgg
    36721 catggcccat gcgcttgccc gcgggggcgt tccggccgcc caggaaggcg accaggggct
    36781 tgcagaagac tccggactcg atggcctcgg ccacctcctc ctccatggtt cccccgagct
    36841 cgccgatgac gaccaccgcg tcggtccccg agtccgcctg gaaatccggc aggaactccg
    36901 cgaaccgggt ccccggcacg gagtcgcccc cgacgcccag gagactggag acgccgaaac
    36961 cggcccggac gatcatggcc gtcacctcgt tggtcaggga cccggaccgg gtcatcaccc
    37021 cgatccggcc ggggcggtag acccgctcga tccagtaggg gaacattccc atcatggccc
    37081 ggcccgggga gatgaccccg gaggtgttcc cccccacgac ccgggccccg caggaccggg
    37141 cggcggcgcg gatctccagg gcctcgtgca gggggatgcc gtcggcgatg gtgaccacct
    37201 tgcggatccc cgcgtccagg gcctccagga cggcgtcctt ggtgaagcgc ggcggaacga
    37261 agagcatggc cgcgtccgcg ggatgctcgg ccacggcccg tcgcacggtg tggtagacgg
    37321 ggacgccctc cacggactgt ccctccttgc ccggggtgac cccggctacg acgtccgtgc
    37381 ccaggtcccg catgtgcttg gtccagaagg tcccctcccc gcccgtgatc ccctggacca
    37441 ggacgcggtc ggaccggtcg atgaacacgc tcacttcgag cctcccgccc caggggccgc
    37501 cccggagccg cgggcagcgg cggccagggc cacggccttg cgcacggcct cctcggtgtc
    37561 gaacagagga tccagaccca gggagcgcag ggtctccacg gccttttcct ccccggtgcc
    37621 ccggatgcag gccacgatgg ggcacttcgg cgccaggcgc ttcaccgcgt ccgcgatccc
    37681 ctcggccatg acgtccgccc gggctatggt cccgaaggtg acgatcagga tgactttggg
    37741 ctcgtttttc aggcagagct ccatggcccg gacggccttc ttgtagttcg ggcccccgaa
    37801 ctccaggtag ttggccaccg agcccccctc gtccacgatc aggtcgtaga ccacggtggt
    37861 gagccccgcg ccggcgcaca tgagggagat gtccccgtcg aactggaggt aggggatccc
    37921 ctccgcggcg gcctcgaagg cggcgtcgga gtcgtagtag tcccggcccg gttcccggcc
    37981 cgggtggcgg aagagggagt tgtcgtcgat ggccagcttc ccgtccccgg ccacgacccc
    38041 gccgcccttg gtcaggaaga gggggttgat ctccgccagc tccgcgtcgt tcttccggaa
    38101 gacccgccag agggcctcca ggatcccggc ggtcttcttg gccgcgtccc cggaaaggcc
    38161 cagggcgtag gctacgttcc gggcccggta gggggagagc ccctccgcca ggggaacccg
    38221 ttcccggacg atcttctccg gggaacgggc ggccaggtcc tcgatctcca cgcccccctc
    38281 ggagcaggcc aggaccacgg cctcggcggc ggcggggtcc acggtgatcg aaaggtagag
    38341 ctcccggtcg atgtccaccg cctcctccac caggatcctg cggaccccgt ggggactggc
    38401 gaagagggcg gccgcggtgt cccgggcctc gggctcggac cggaccagct tgatcaggcc
    38461 cgccttgccc cgtcctcccc ggaggacctg ggacttgagg acgcagggga agcctgcttc
    38521 ccgggcggcc cgggcgaccc cctccggatc cgtcgccagg acggaccgcg ggacggggat
    38581 gccctcggcc ttgaagacgt ccttggcctc gtgctcgtgg agtttcatgt ccgcctcact
    38641 tgccgtactt ggcccagagg gagccgaaga tctcctcgtc cgagggccgg ttcggcgcca
    38701 ggggcagggc cagccaggtc acgttcacga agtgcttcca gtggatgttc tcggtggtga
    38761 tgttcccccc ccaggtgccg cagcccaggg tgaccgtgga gggcatcccg ttggcgaagg
    38821 tgcccccgtt cccggaggcc atgccctggc gcaccatgat gcggctggac cgcagggtct
    38881 cgcccatggc caggatgttg tcctcccggg aagtgaagat ccccgaggag tggccggtgc
    38941 cgcaggcgtc ggtgatcagg accaccgcct ccagaccgga ccggaagtcc gggcacttcc
    39001 acagggccag gaccggggag atcttctcct gggcccactt ctccggggcc ggtccgccgg
    39061 gccggggaag ttcggcctcc accatcagga tcttcgtgcc cggggccacg gggatccctg
    39121 ccccggcggc gatcgactcg gcggagcggg cgatcacctt ggggttcaga gcttccttcc
    39181 cgtcggggcc gaccggccag agccaggagc gcagtttctc cttctccgcg ggggagcaga
    39241 gatgggcccc cttggtccgc atagcccgga cgaaggcgtc gtagacgttc ccgcaggcca
    39301 ccacggagtt ctcgctggag cagcttgtgg cgttgtcgaa ggccttgctc acggcgatct
    39361 tggacgccgc gtcctcgggg tccgcgtcct ccgccaggag ctggaccgcg ttcccgggcc
    39421 ccacaccgta ggcgggggtt ccggaggaat aggccgcctt gaccatgggt ccgcccccgg
    39481 tggccaccac caggtccacc tggcgcatga gctccccggt tcgttctatg gaaggctcct
    39541 ctatgcactg gaccaggtcc tccggggccc cgaccgatcg caaggcctgg cgcatgaatt
    39601 cgcaggccag cttggaggcc cccttggcct tggggtgggg ggcgaagatc acggcgttcc
    39661 ggcccttcag gatggaaagg ccgttggagg agggggtggc ggtggggttg gtcaccgggg
    39721 ccagggcccc gacgaccccg acgggcttgg cgaacttgag gaggcccttc gcctcgtccc
    39781 gctcgatgag gcccacggac ttggcggtcc ggatgtcccg gaggaccccc atcaccttgt
    39841 tcttgtgctt ggtgatcttg tcctcgtaga ctcccatgcc cgtctcgtcc accgcggccc
    39901 gggccaggcg ggcgatgttc tcgtccttgt agaccgacca gcccacggcc agacagacct
    39961 c
//
LOCUS       DAOTEI010000009        39307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_685_length_39307_cov_5.513248, whole genome shotgun sequence.
ACCESSION   DAOTEI010000009 DAOTEI010000000
VERSION     DAOTEI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 39307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39307
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_39307_cov_5.513248"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2709)
                     /locus_tag="P5313_01585"
     CDS             complement(<1..2709)
                     /locus_tag="P5313_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HRY79085.1"
                     /translation="MTVTFKLNGRPVSWDCPEGESLKAALRRKGIVSVRDGCDGEGSC
                     GLCAVLLDGRTVNSCTLLAAQAEGREIYTVEHFSKDRKLSVVQRALIDAACVQCGYCT
                     PAVVLAVHSLLERSPEPTKQEVKDALSGTLCRCTGYEQFHNAVRIAAKRLKDPGYAEA
                     AAPEFRPDLRHVGKDREKVDAAALARGEKAFVEDRVDSDACHLALLTSPHAHAWIKSI
                     DASAALELPGVVAVLTHENCPDRVYTTAGQGFPEPSPHDQRMFSRKVRHVGDRVAAVL
                     AETPDQARTALKALKVEYEVLKPVFSIAEARTPGAPVVHSGEVRYVSGEAPAGTSTAG
                     DGRDDPVVYQFPLHADPHRNLAASVADGIGDVEKGFAEADVVVERTYKGNRVQCTPME
                     PHVVYAKVDGGRLVLHASTQVPWHVRRIVSRAVGIPENKVRVIKERVGGGFGAKQDIV
                     LEDVAGYLAWTTGRPVFFRYDREQEFISSRTRHLMEITVKLGAKKDGRLTAVLMKVDA
                     DTGPYGQHCLTVPMNAVSKSLPLVLCDNARFEVKSYYTNLTPAGAYQGYGAPKGSFAL
                     QTALAELAAELGMDQLDLIERNRVRSGSRIEILRSLGEGRPGAAVTLGECGLGEMIGR
                     GRKAFGWGGAKPATEDPDWKVGRGAVIVQQGSGLPGLDAANAEVRLLADGTVLLLSGG
                     ADLGTGLDTVTAKAAAEILGLDMEDVAVISGDTDVTPFDKGAYASSGTYFSGNAAVKA
                     AEDLRAKVLAAASAVLGEPVSDLAIVHRGIVRGRKGEVTLAKLAHMSTQGEHHGDLVG
                     IGGFKTEHAAFPYGAHFAEVAVNVRTGDVKLRRFHAYQDCGTPINPALALGQIYGGIL
                     KAVGHSLYEELLYDKDGRCVNPRFLDYKIPTIWEVPEDFHVELVPVA"
     gene            complement(2706..3500)
                     /locus_tag="P5313_01590"
     CDS             complement(2706..3500)
                     /locus_tag="P5313_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY79086.1"
                     /translation="MKYIRAASLDEADRVLASDKRARILAGGTQLLTSEFRDREFVAV
                     DAGSLLPRGLERTAEGLTRIGAGAVFQDLADSPVPSRILSEAALGMANRNVRNAATVG
                     GNLGAARSCSSLVPALLVLDARVRIHGAAETRPLADWLAAPSGIVTWIEIPASPGLRA
                     AYARWSRTACDLSVLTCAASYRLQDGSLRDVRIACGGLDARSRRFPELERLFEGRPLP
                     GRDEAAAAVAPALKPIDDLRGSAAFKRLRASELVAEALLTAREVSR"
     gene            complement(3497..4876)
                     /locus_tag="P5313_01595"
     CDS             complement(3497..4876)
                     /locus_tag="P5313_01595"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HRY79087.1"
                     /translation="MIVLKDCLCIVRPPGSPGGNLAGADVHIDGNRIVRVGKNLPVPE
                     GTRVIDASRHVVTPGFVNTHHHFYQTLTRNLPAVQNAKLFEWLVYLYEVWKGIDEEAV
                     YWSSRLALAELVKTGCTCTTDHHYLYPRDFSQDLPGIQFQAASDIGVRFAPTRGSMSR
                     SKKDGGLPPDSVVQDEDTILAQCEETIRKYHDPAPDSMRRVALAPCSPFSVSEQLMKD
                     SAVLARKYGARLHTHLAETGDEDDYCVKIYKRRPLKVMEDCGFLGPDVWYAHGIHFND
                     QELDVLARTGTGVAHCPSSNMRLASGICRVREMLDRGVNVGLAVDGSASNDASDMLGE
                     MRQALLLQRVRYGSAGITAPEVYGIATEGGARILGWDRLGRIEEGWLADIALFDVQKM
                     EYAGALSDPAAALLFSGYDHGADYVFVNGKAAVVQGRLVGEDEEEIRDNANRIAARLY
                     AKAGVAGWS"
     gene            complement(4873..6336)
                     /locus_tag="P5313_01600"
     CDS             complement(4873..6336)
                     /locus_tag="P5313_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY79088.1"
                     /translation="MKIDLSINEAQRKKNIQRCKEKGILLPTYAQMRDPKKIPDSIKA
                     DLKGIGLWDVHSRNLFRVNWHNEPKEKGGVFGDVNYFELPRELTGTKARIGAVLGKWF
                     PTGAHKVGAAYSCLVPGLVTGQFDPTTKKAVWPSTGNYCRGGAYISRLLSCPSVAILP
                     AEMSRERFEWLATMAEEVIATPGCESNVKEIFDKCVEIEKTRPDAVIFNQFDQLPNHL
                     WHYTVTGPALEEVYGKMNADGKSRLALSLYSSGSAGTLGAGSYLKEKHNGARVGVGEA
                     LQCPTILENGFGGHRIEGIGDKHIPWIHNLRDTDVAVGVDDEITVRLLRLFNEPAGRR
                     ALVEAGVPQAAVDRLDLLGISGIGNLVATIKAAKYYELGADEIALTVFTDSLQLYGSR
                     LKELAAERGPYDQRQADRDLEMLKSLSVDYVLDMTHTDRRRVHQLKYYTWIEQLGKDL
                     AELNAQWDDWRGYWGELHGEVGALDALIEDFNKEVLR"
     gene            complement(6473..7627)
                     /locus_tag="P5313_01605"
     CDS             complement(6473..7627)
                     /locus_tag="P5313_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY79089.1"
                     /translation="MRWFYRCPTCGAEYEMEPDRYLCSSCAPKTLPDRPLEGVLECAW
                     AGVWEGDSRSVPLPVEPEWFPPVPVGDTPLWAPERLRADTGFKGLWIKDDTCQPTGSF
                     KDRASFLVAAYARKKGIREIALASTGNAASSMAGIGAAAGLKITVFLPKAAPVAKRIQ
                     VLQYGAELKTVDGTYDKAFDESLAYTKATGTLSRNTAYNPLTIEGKKTVSFEILSDLA
                     ARGAPAPDHVFVPVGDGVILAGVYKGFEDLVRLGRLSKVPVVWACQAKGSSAIARALS
                     AGGFGDPEPSATIADSISVDVPRNGTLALAKLTKYGGRAVVVTDEEILSAQRTLASGT
                     GLFAEPSSSAAAAGFLKVKGELDPSSTVVLLVTGSGLKDVKSAAHGLGIS"
     gene            complement(7642..8856)
                     /locus_tag="P5313_01610"
     CDS             complement(7642..8856)
                     /locus_tag="P5313_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HRY79090.1"
                     /translation="MDSNAQKVRAKASSYRDETADILSRIIQVPALSAKEKERIALLK
                     SMCEKAGFDEVKVDGLGNLLARVGSGKKILAVDAHIDTVDTGDPAQWKNPPFSGLVAD
                     GLVHGRGASDQLGGAASMITAGRILKELGYAGDYTVWFAFTIMEEDCDGLCWKYLIEE
                     EKLVPDLFVSTEPTSLRLYRGHRGRMEIECRFQGVSCHGSAPERGKSAAYMASRAALA
                     IEKLNENLKPDTDNFLGKGTVTVSRMDVKGPSLCAVPDQGMIYLDRRLTWGETKELAV
                     EQVKKALSEAGLSNFEVKVPYYEIPSWKGTKYGQDLYFPTWKIDADHALVRAGVAAHK
                     ALWGSEPVVDKWTFSTNGVAVCGRHKIPTIGFGPGDENQAHAPNEITRVDDLEKAAAF
                     YATLPYALEGKL"
     gene            complement(9004..10335)
                     /gene="ssnA"
                     /locus_tag="P5313_01615"
     CDS             complement(9004..10335)
                     /gene="ssnA"
                     /locus_tag="P5313_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011218649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HRY79091.1"
                     /translation="MLIFENVRVITWNPAAVSEPRDVVLREAAEGGTVAEAGANLKAK
                     YPAARAAGSGGYLSPGLVCSHTHLYSALARGILANIRPSKDFGQILAHLWWRLDRAID
                     EPILRASARAGLADAAACGVTSVVDHHASPGFIDGSLDVIRAAYDEIGLRGLVCYETT
                     DRNGLEGARVGVRENERFARVLDAERKAGKAPRVEAAIGGHALFTMGDETLELSAQAV
                     RSTGRGFHVHAAEDRYDASDSRHRYGADLVERMEKFGLLGPKTIVGHGLWLSPSEVEL
                     LNERDCFLAHNARSNMNNAVGYASLLPGLKNPVLGTDGMGADMAEEFKFAYFRHREAG
                     GPWWPGDFLGVLDRGNRLMERYMAGTRFGRVEPGYPADLVHWDYDPPTPLSAENLAGH
                     LAFGLSSRSVRTVLSDGAFVVRDRAPAFDAAGIAADGRVQAARLWKRMDTL"
     gene            complement(10329..13544)
                     /gene="ygfK"
                     /locus_tag="P5313_01620"
     CDS             complement(10329..13544)
                     /gene="ygfK"
                     /locus_tag="P5313_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HRY79092.1"
                     /translation="MSTSKVMETTPMGALIGRMAGEYKAKKSVYDLPEALFRKVFEDE
                     PASPGFEVLGSRILYPIGPAAGPHTQIAPNLVASYLGGSRIFELKTVQVNDALEIAKP
                     CIDALDEGHNVEWSTELSLVQARDEYLRGFMALHVLKALFAERGAGDFLFNMSVGYTL
                     EGIKSPTMDAFIEGMRAPASTPFWGAALAELRAAVTSPAFREAFGAEGAARAAAAVDS
                     MPAAPVHSVTLSTMHGCPPAEIERIGRYLIEEKGFSTYIKLNPTLLGYDRVREILDRT
                     GWTDVEIQRPGFEHDLQWPDALALITSLAAAAKRKGVRFGLKLSNTLANVNVGDRLPG
                     QERYMSGRALFPITVRLAADLAAAVPGPLPFSFCGGVSALNAADCVRAGLGPLTVATD
                     ILKPGGYLRLADMARSAVSALPAAPKGPDARSLAALAEAALGRPEFRGDWKEGEAAID
                     APLPEFDCFDAPCVRACPVNQKVPEYIRLSARGKDEEALALILADNPLPYITGTLCDH
                     VCQEACCRNDYEGPVEIRGVKLACARAAEIPPKPRARRKDFPGKAAVIGAGPAGLACA
                     YWLAREGVPVTVFEKAASPGGVPANVIPPFRIPRKDIERDIERIRSMGVEFRFGAEVR
                     DLGSLRADGYSAVFVGAGSPAPRDLPLTGSGVRVVDALEFLAETAEGPGPFAAVRHVV
                     VAGGGNTAMDAVRAASRIPGIREVRLSYRRTVREMPADREELHNALSEASAVNSASGA
                     ASPPTALMELTLPESAAPGTLTLRRMTLGERDASGRRSPVPSGETVDVPCDLVVAAVG
                     ETPDRAFLEALGVPVGKDGRPKANLETGSVGVPGLYVGGDALRGPASIISAVADGRRS
                     AAAILRAAGIEPEGAWGSYAPPAPDRDKLARRGSQDPAAAAREELRVPEGAGARAFTA
                     AQAERCLECDAACLRCVEVCPNRANTFVAVPTGTGFTQSLQILHVDALCNECGNCGFF
                     CPWRGEPYRGKPTVFASAGALEASRNAGFAFTGKAGAPDLVLRAEYEGEVRTLPHAEW
                     AVASAASAPGAAGPAGAAARMAALARIVGREHPYLAGGDAC"
     gene            complement(13541..14923)
                     /locus_tag="P5313_01625"
     CDS             complement(13541..14923)
                     /locus_tag="P5313_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY79093.1"
                     /translation="MIVKNGLLALRYERDFLRRDFRVGPDGRVTEIAETLTPQPGEEV
                     LDARGLQVLPGAIDPHVHFDEPGFTHREDFLHGTSEAAKGGVTTVVDMPCTSLPPVTT
                     RAALENKLSFVSKAAVVDYAFFGGVSGHTVEESLSEHMEALAPEVVGFKTYFISGMKT
                     FTRVTHDDFGRIVRRGAALGRPILLHAEDLDCVTAAEARLRKARGAEAPRWSDYVGAR
                     SEDAETVAAASAATLARGNEAWLHVVHVGTAEAAELLAASGATCETCAHYLAFSAEDF
                     ESLGSSLKTAPPVKAPGQAERLWALLSGGAISFVTSDHAPAPEEEKNTGDLWTDYGGI
                     PGTGTWAPFLYSEGLLGGRLSLSRYLDVTARAAAERYGLSGRKGSLEAGKDADFILAD
                     PRGTTVVEGRTLLSKGKITAFEGMRLSGRFLATYVRGRLVYDAARADRNEHPICAAPG
                     SGRFLRWGYR"
     gene            complement(14920..17136)
                     /locus_tag="P5313_01630"
     CDS             complement(14920..17136)
                     /locus_tag="P5313_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY79094.1"
                     /translation="MKSWNPESVQNVLGRTKYIDDLPEPAGLLHGQPVVSKSAHGRLR
                     SLDPSAALALHPSVRVLSARDIPGENELGTAVSDEPLLVADEWTYRGQSLALVLADTR
                     SLARRAAALVKIEGEELPAVTDPREAHARGMIILAPRTMRTGDPEAAFRSCAVVVSGR
                     VESGGQEHVYLETQGAVAQVRDGGGIFVLSGTQSPTGVQEAVARNLHLPMNAVEVDAP
                     RLGGAFGGKEDQAASWSALAALGAYVTGRPVKVYLNRKDDLTFTGKRHPYSSDYRLGL
                     DSEGRILAFEVDYYQNSGACTDLSPAILSRTLFHAASAYRIPNLRATGWMCRTNLPPF
                     TAFRGFGAPQGIFVMEAALHRAAETMGVDPIELQRKNLLRTGDTFHYGMPVAEARTER
                     SVDRALQRSGWDRRRADIDEFNRAHRDRKRGMAALPLCFGVSFTKLMMNQGGALVHVY
                     RDGSVGVHTGAVEMGQGVNRKIQKVVALTLGIPEGRVRIERTRTAAVANTSPTAASTG
                     TDLNGMAALVACEEIGKRLSAFRSGSGLADAPWETLVAKAHEARIDLSAHGFYATPGL
                     FYDGKAERGSPFAYHVYGCAVLIAEVDTVRGTYRIASATLVHDAGNVIDEVVDRGQTE
                     GALAQGLGWALLEDLRYDETGRLLSDTLSTYKLPDAHFLDFPVDLEFLGDAPNPRAVL
                     KSKAIGEPPFMYGIAGYFAVLDALKAARGDRLRAPGIHDLPLTPEKALDYLTGVIP"
     gene            complement(17133..18545)
                     /locus_tag="P5313_01635"
     CDS             complement(17133..18545)
                     /locus_tag="P5313_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY79095.1"
                     /translation="MKRRLVFRVNERLVDAEAWEETLALDYLRRDLGLTGTKEGCREG
                     DCGACLVLLGERGGDGPSWKAVPSCLLALGELDGRHVVTIEGIAAGGLTPVMEAFLDE
                     GASQCGFCSPGFIVALTAFLVEGHIDPVRALVSIEGNLCRCTGYGSIRRAAERLTRDF
                     ADLPADLGARLAVLGDRKVLPGSLAALMTSLPEPGSRPAAAPKGLNLGGGTDWFVRNP
                     DPEPGTGVAFTDRQAGLDRIERTGDFLSVGAAVTVRSFFEDPRIRAHAPGIEAFEAQM
                     ASLPIRNRATLAGNVVNASPIADMTALLLALDARVRLRSASGARELSLADFFLGYKKT
                     ALRDGEFLETLLLPAAAGPVRLNFEKVSKRESLDIATANTALALRMEGDRIAWARLSV
                     GGMAAVPLLARKASAFLVGKSPDAAAARETARLALEDCSPLSDVRGSAGYRTRLLERL
                     VLAHFVRLFPDKGLLEEFWP"
     gene            18808..21039
                     /locus_tag="P5313_01640"
     CDS             18808..21039
                     /locus_tag="P5313_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY79096.1"
                     /translation="MLRSPFLLLGLVWMGAAAAAQGVPAAASTGTRAELREWRYVPGD
                     RPEYADPGYDDSGWASVRLPARLDLDAGRGWLRGEFDGSGMPDGEVYLLLGRVDAAAE
                     VYLNGSLIGRRGTLVPEFTVPGNQPGVFLLPSRLWNPRGGNLLALRLAAPASALDASG
                     FQIGDGSAADYERRVIGFFNFELYTVLGALCAFIGLYFLALWAGRPADTPNLWYAVSS
                     FAIAFYFAEIGATYPILEYTLNRALAKACLTVSMAALVAFFLSFFRLRLPRILVALLA
                     LVPAASVVSYLVVRGDYVRVDAMFNRNLIFVQAAIVFIVFVTVREVIRGNREAVPILV
                     GVVLGVGFGTHDVVYSALGRKPVAWLQGIGFFALNLSLFVTLTLRSSRLHAELRRYSA
                     DIERKTGQLADYIGRIEETADSVSAITAEIDSDAAKASESADKLAAEAGRIGANAERQ
                     ARAARESGQAVERLGRSLGLVRTGVDSQAAGIEGSAEAVAVVADAAASVAGSVSSTAD
                     FARGLGLTVERGRDSSRTLGEAIDRIKEAAGRVVDIVGAVEEFAERTNLLSMNAAIEA
                     AHAGAAGRGFAVIAGEIKSLAGASAERAARIRDSIADIVRRIENGVEANAGLAESLDS
                     VAEGARTALEGILSVSGSLEAQRGATDRLRTSLRDLADSAASIRAEAERQDEEGGRIR
                     ERMSELVSISDELGQSIGGIARENAEIADTIKRLAEVSRDGKEAVTGLRRLLDAREGP
                     ASG"
     gene            complement(21036..22754)
                     /gene="ade"
                     /locus_tag="P5313_01645"
     CDS             complement(21036..22754)
                     /gene="ade"
                     /locus_tag="P5313_01645"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRY79097.1"
                     /translation="MVSKEQIDAARGLVPCDVNFVNCSVADLFRGEVRPGLTVSVHKG
                     YVVGVGDGLAARTEVDLDGRVLVPGLVDAHVHIESSLLSPSEYARLVVPRGTTAVVAD
                     PHEIANVLGYDGMTYMLRASEGLPLDVYLMAPSCVPATDFDSSGAALFASDMYPFLRE
                     PRVLGLGEVMNFPGVLAKDPQLLDKIALFWDSGKAVDGHAPELRGRDLSAYAAAGIRS
                     DHECVTAEEALEKISKGMYVMIREGSATRDLARLVPAVTPRTAPRFLLCSDDRHPTDL
                     AEEGHIDRSIRLLVDAGVDPLDAIAMSSLNAARYFGLRNSGAIAPGYKADFVAASDLR
                     AFEAEVVIKSGQIVAEGGRLVRAVENRPAVLRDSVNIKWLTEEDFRIPAEGRRIRVIE
                     ARPRTLITGEIQTEPPVREGLCVSDVSRDLLKIFVIERHKGSGNIGKGFITGLGLKRG
                     AIGSTISHDSHNMILVGADDASIFKAARHLNKIGGGMVVTDGDRIVLDLPLPIAGLMS
                     DRDAMWVVERLRAFDELFRAEGLTNESPLMAISFMALPVIPKLKITDRGLVDVERFAP
                     VPLFVD"
     gene            22947..24233
                     /locus_tag="P5313_01650"
     CDS             22947..24233
                     /locus_tag="P5313_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRY79098.1"
                     /translation="MEKFFKLKEHGTTVRTEVMAGITTFMTMAYILAVNPGILSAAGM
                     PAGGVFTATALASLIATLCMALLSNLPVALAPGMGLNAFFAFAVVLGMGYSWQLALTA
                     VFVEGVIFILMSLFNIREAIVRSIPANIKSAVSVGIGLFIAFIGLQNAGLVVNNDATL
                     VGLGNITKGAPLLALIGLVIMGVLLIFKVKGALLIGILATTIIGIPFGVTVIPKGWNP
                     VSAPAAPLLFQFQFDKFFTLDFWIVLFTFLFVDIFDTIGTLVGVTTQAGLISKKGEIP
                     RVKEALLSDAIGTVAGACLGTSTVTSYIESAAGVAEGGRTGLTSLTTAVLFGVALFLS
                     PLFLLVPGAATAPALILVGLFMLSPVKDLDLKDFSEAIPAFITMLFMPLTYSIAEGLV
                     FGILAYILLKLCTGKAKEIPVVTWIVGFFFVLKHVL"
     gene            24305..25147
                     /locus_tag="P5313_01655"
     CDS             24305..25147
                     /locus_tag="P5313_01655"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRY79099.1"
                     /translation="MRLRLPFAPAFLAALAVLSAAAPAGAQSDVRVPDWMAYVEGGTY
                     LQGSPDAEAGRSFDEGPRREVNLSAFLLAKREVTFDEYDEYCAASGKSRPDDKGWGRW
                     SRPVINVTWRDAVEYCNWRSRKDGLAPAYALNGDTVTCDWTAEGYRLPTEAEWEYAAR
                     GGKRTRGFPYPGGSDPEALAWFSLNAGNRTHPAGGKAANELGLFDMAGNVWEWCWDWY
                     AGYPAGKASEPRGPEGAALKVVRGGGYFNVASNLRPAKRWFVDPAHKFDYLGFRLARS
                     LPRR"
     gene            complement(25409..26107)
                     /locus_tag="P5313_01660"
     CDS             complement(25409..26107)
                     /locus_tag="P5313_01660"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRY79100.1"
                     /translation="MPVETKKVLWTVISVGLFLVVFFGTALLLFAPRKGAPQAPAAIG
                     NEAPPRAVEPDEYLRNPAPAPDPAAPLPAAPGEVVIVYGENPPAAALPGAAAPGSPTG
                     AAQTAPAGTALPAPAKSGSYTPAAAPAAKAPAAKPSAPAPKPKTVTVTEWWIQASSPT
                     SRSRAESLQAELARQGLSSVITLKDVDGTTHYRVRIGPYTSEKEAIGWRDRVRALPDC
                     AEAYVSKTTVQRTL"
     gene            complement(26238..26834)
                     /locus_tag="P5313_01665"
     CDS             complement(26238..26834)
                     /locus_tag="P5313_01665"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRY79101.1"
                     /translation="MILGLTGGYCAGKNLVARLLAERGWAACDVDVLGHAALESRLAE
                     TAELLGPGILRPDGSPDRREIGRRVFADPALLARYEAVVHPALFALLDEILRDAGDRD
                     LCLNAAILYKLPQVARCTAVLEVRSPLLARIRRGRARDGLPPGAILDRIRRQRPLWGR
                     RREYPGPVLVLRNSGSEALLARRLDAALERLGRGRPTS"
     gene            complement(26834..29653)
                     /gene="polA"
                     /locus_tag="P5313_01670"
     CDS             complement(26834..29653)
                     /gene="polA"
                     /locus_tag="P5313_01670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRY79102.1"
                     /translation="MESRKPALYLLDSYGIIYRSYYAFINRPLKNPKGANVSAVYGFF
                     RFLFALFEERNPGAFAAVFDSAVPTFRHEMYTAYKATRQKTPEDLHAQVPLVEEILRA
                     LGIPVLKADRYEADDLIAALAERCRAEGRECWIVSGDKDLLQLVGGSVRALRPEKDTA
                     CRSLGPEEVEAEWGVPPARILDYLSLTGDSSDNVPGVPGIGDKTARKLLTEYGTLDGL
                     YERLASVKPDGIRAKLEAGRESAYLSRTLITLASDAPLPVAGPSELEVGTLNRAAAGP
                     IFLREGMRSLAGKAGVAGGVTAATDLFEAAAPGVPVAAPGVHGPEAPEAAAAASPFPP
                     AVDPAVPAGLSGPGEYEAVTDEASLDRWLDAAARAGTFAFDCETDALDDLSALPVGFS
                     LAVEPRKACYVPIRCPEGTKIPEAVLKAKLGALLAREDLVMAGQNLKFDRHVLENWGV
                     AARCRPWDTMVAAWLLDAERSAFNLEALAESYLGYRGIEYDDVVPKGSTFDKVPLAQA
                     SGYAAEDADLTLRLKLRFEPELVRAGLADLFRDVEMPLLPVLGDMERAGIRVDPEELR
                     RYGRELDVALRDIEAEIYRLVGHEFNIASTKQLQEVLFTERKLRTGKKTKTGYSTDIS
                     VLEELAREDPVPERILRHRTLSKLKSTYVDSLAELAGPDGRIHTHFVQTGAATGRLSS
                     RDPNLQNIPIREEEGRRIRHAFSAESGRVLVSADYSQIELVVFAHLSGDPELRGAFAE
                     GADIHRRTASLIFGIPESEVGPDQRRIAKTINFGVVYGMSAFRLSNELGIGRGDAQKF
                     IDAYFERYAGVRRFMETTIAAAEKDGYVRTLLGRRRPVPAVVSRNKTEKQAAERVAVN
                     TPIQGSAADIVKLAMLRVRRALDRSVPEARLLLQVHDELIAEVPEARAAETAEVMKRE
                     MEGAVRLSVPLRVDVETAVRWGEMH"
     gene            complement(29770..30153)
                     /locus_tag="P5313_01675"
     CDS             complement(29770..30153)
                     /locus_tag="P5313_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79103.1"
                     /translation="MPDRLVLCVDDEAVLLLALRHRLESVLGAGYRIETALSGESALA
                     ALETIRAGGGEPAAVVSDWRMPGMKGDEVLRAARARFPRTRLVLLTGYADREELLALD
                     SELGLAGILQKPCDVRELAKLIRGQ"
     gene            complement(30146..32035)
                     /locus_tag="P5313_01680"
     CDS             complement(30146..32035)
                     /locus_tag="P5313_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY79104.1"
                     /translation="MKPAPLRVLCISALLTSFAPSTLPAQKGPVIWGLVPNAPPTTYV
                     DAAGKPTGFFIELYSRIMDELGIDYEFRVASFSELYPRLVSGEIDFFTTLLKNPEREA
                     LFVFPDQGVSAGWSQLFVAHGTVLQSVLDLQHKRIGVVTDDRNGAAFRTYVDSLAIPC
                     EITEYPDFDQLVRAVSGGEAFAGVQSNWFVSAERRVQPTTVVFSPFKAYPVLSRNSGR
                     RAEFDAVVDRYAEIVADPDSYYYELQRKWLGHERTETAVIPAWVPAVVATLLAASLVA
                     AFVIRALTSRLRRVNADLERKVEERTAQVARSEKLAALGGLAAGLAHELNSPLQALRL
                     AAEGLAEEPVLPVLSGPESSQVNHILEAARGVRAAVRADSGSLRDILAARLETAGLDA
                     DGRLAARLSDLGIPDPDEGTLALVRDAPETVIRAAREARARDAYRVALADSLDRITTV
                     VQALRVYTHRDHRGVPTVADLTRQIDAVLELSKNRLGTGIRVLRDYSGLPPYRCYADK
                     LQQVWMALIDNALDALGETGTLAVRGEAIPEGIRVSVEDSGPGIPEELAGRIFEPFFT
                     TKPAGEGFGLGLATASEIIQTHRGRLAFDSRPGRTVFTVSLPSAGIVSAGGSEERSYG
                     TDTDA"
     gene            complement(32131..32646)
                     /locus_tag="P5313_01685"
     CDS             complement(32131..32646)
                     /locus_tag="P5313_01685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRY79105.1"
                     /translation="MLHPVTKKQNNSRMCFVCGLANEHSLKASFYETESGELVAKIKP
                     TELHQSYPGRMHGGIAAAILDETIGRAIAIGKDGQVWGVTVELNTKYRKPIPLDQDLW
                     IVGRVTKDGSRFFEGSGEIVLENGDVAVSAVGRYLKVPIDKITDSAMDERDWFLHRHA
                     EDPETIEVPGR"
     gene            complement(32811..33275)
                     /locus_tag="P5313_01690"
     CDS             complement(32811..33275)
                     /locus_tag="P5313_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgN"
                     /protein_id="HRY79106.1"
                     /translation="MALTEAALEERKSLLRRFRELLQKQRDKFHHYLEVLDHQKADIE
                     SGDVDSLVAHVEMEQGIVGEIFAFQKVIDPLEDLYRAAYPSAAEDDIPALKATLEDLK
                     TEVVERNTENRKLLKQRMEMLRHEIMSVNNPYAKKRSVYADAGEAGLVDLKG"
     gene            complement(33288..33722)
                     /gene="fliS"
                     /locus_tag="P5313_01695"
     CDS             complement(33288..33722)
                     /gene="fliS"
                     /locus_tag="P5313_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRY79107.1"
                     /translation="MTQNPLTAYRETRVKTASPGQLIVMLYDEAVKQCDIALEIFAKD
                     VKKHPQYIEGAHKALSKVQDIITELTAALDFESGGEIAQNLFALYLYFNRELLAANID
                     KDGSKLRAIRGMLDELRTAWATAAAQTAGSGSEARAGVNIAG"
     gene            complement(33805..34311)
                     /locus_tag="P5313_01700"
     CDS             complement(33805..34311)
                     /locus_tag="P5313_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRY79108.1"
                     /translation="MDPIFDRLETLLKSLWNQDRDPFAGDRPAEGRVRPSSGDSDYSD
                     ALDELDAYLKDDRQAQERLEAERRRRAEEEERRARNRYSSGAAGRSPGADPSARLSGD
                     YKILGLAFGAPMAEVKAAYKRLLKQHHPDRHGASPEAQKKATEFSARLNAAYARIETW
                     TSTGKIPE"
     gene            complement(34565..35533)
                     /locus_tag="P5313_01705"
     CDS             complement(34565..35533)
                     /locus_tag="P5313_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRY79109.1"
                     /translation="MGLRGRRWGTAATVLAAGMLYGTTGTAQALAPGGADPSSVGLLR
                     LALGGLALLPFAAARSGGFRVLLRGASPWVLLAGAGLAGFQVLFFRGVIAAGVALGTV
                     VAIASGPVFAGALGAAVFRERLSPAWWVSTALAAAGCALVSLGKSAAPAPAAGIALAL
                     GAGASYALLGLGIRKSSAALPSLGAVTLGLLAGSLLLVPVYLASGSGLGWVLSPRGAL
                     TAAHLGLLTTALPYFLFGRALGILPMGWVYTLGLSEPLVAALLGILYLRETPSPAALA
                     GIALLTAGLAVSGAAASGKPSVAREEGSPGITTESTERTEAYDAGL"
     gene            35595..37397
                     /locus_tag="P5313_01710"
     CDS             35595..37397
                     /locus_tag="P5313_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRY79110.1"
                     /translation="MAARNDYDESKIKTLSSLEHIRLRTGMYIGRLGTGTNPDDGIYI
                     LLKEVVDNAVDEFIMGAGRRIEVRIEGGRVAVRDFGRGIPLGKVVECVSVINTGAKYN
                     DEVFQFSVGLNGVGTKAVNALSSRFRVVSHRSRKCLEAVFKRGRLVSQAESSTREPDG
                     TLVEFEPDPEIFGDFAFNMEFVEKRFKNYAYLNTGLTLSLNGNDFSSENGLLDLLKDE
                     VGEDTLYEVGWWRGDKIEFAFTHTGNYGEENFSFVNGQYTSDGGTHLSYFREGFLKAV
                     NEYFQASYRGEDVREGIAAAIAVKLQNPLFESQTKNKLGNAEIRPWILHEVKRGVDEF
                     LRRNPQAAKRIQDKIVSNEKLRTELNIVKKEAKEAAKKIELKIPNLKDCKCHLGDGDR
                     GEVSMIFLTEGQSAAGSLVSARDVYTQAVFSMRGKPENMYAKKRTAIYKNEELYNMMM
                     ALGIENDIEGLRYARIIIATDADYDGYHIRNLLLTFFLTYFEELVVQGRVHIFETPLF
                     RVRNKKETRYCYSPKERDEAVKVLGATAEVTRFKGLGEISPKEFGQFIGPDIRLVPVD
                     VQTLKAVPGVLGFYMGKNTPERRDYIMKNLVNDL"
     gene            37411..>39307
                     /locus_tag="P5313_01715"
     CDS             37411..>39307
                     /locus_tag="P5313_01715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRY79111.1"
                     /translation="MAYVKKLFDDNFLFYASYVIKDRAIPDLDDGLKPVQRRILHTLF
                     EADDGKFHKVANIVGSCMKYHPHGDASIYSALVVLANKELFIDRQGNFGNLYTGDEAS
                     AARYIECRATPLAKEIFHNPKITEYVDSYDGRNREPVVFPAKIPVALVLGADGIAVGM
                     STKILPHNPVEVLQAEIDCLRGRPFRLYPDFPTGGLADVSEYEDGAGRVRVRARLDTS
                     DPKRIVIRELPFGSTTESLIASVEAAAKSGRLKIASISDFTTETVEIEIKLARGVYSQ
                     ETVDALYAFTECEQAVSCNLLLIKDGHPTLMTVTEVIRHHARRLVGILKAELELEARE
                     LADEIHARTLERIFIEERVYKAIERMRTQEAVRKAVIDGLAPFAKEIRREVTEEDVER
                     LLKIPIRRISLYDIERAKAEMARLLERLKEVKYHLAHLTDYAVGFLTGVMKRLEPEWK
                     RKTEVAGFRKVDVKEAARRDVALRYDGQTGYLGTSVSTGDSLFDVSPYDKVLAVRRNG
                     IYTVFPVPERAFVDQGLLYCGLAEKELLADVVFTLVYRDAGTGHPHIKRCQIDAWILN
                     KDYQLVPEGDQILCFTTLPQGTLTVKYAPKPKTRVLQEKFKIEDFGVKGLKARGVRLA
                     HREALAAE"
BASE COUNT         5978 a        13425 c        13775 g         6129 t
ORIGIN      
        1 cgccacgggg acgagctcca cgtggaagtc ctcggggacc tcccagatcg tgggtatttt
       61 gtaatccagg aaccgcgggt tcacgcagcg cccgtccttg tcgtagagca gctcctcgta
      121 gagggagtgt cccacggcct tgaggatgcc cccgtagatc tgtcccagcg ccagggcggg
      181 gttgatcggg gtgccgcagt cctggtaggc gtggaagcgc ctaagcttca cgtccccggt
      241 gcggacgttg accgccacct ccgcgaagtg ggccccgtag gggaaggcgg cgtgctcggt
      301 cttgaagccc ccgatgccga ccaggtcccc gtggtgctcc ccctgggtgc tcatgtgggc
      361 cagcttggcc agggtcacct cgcccttcct cccgcgcacg atgccgcggt ggacgatcgc
      421 gaggtccgag acgggttccc cgagcaccgc cgaggcggcg gccaggacct tggcccgcag
      481 gtcctccgcg gccttgaccg cggcgttccc ggagaagtag gtcccgctgg aggcgtaggc
      541 gcccttgtcg aagggggtca cgtcggtgtc cccggagatg accgccacgt cctccatgtc
      601 caggcccagg atctccgcgg cggccttggc tgtcaccgtg tccaggcccg tgcccaggtc
      661 ggcaccgccc gagagcagaa gtaccgtgcc gtccgccagg aggcggacct ccgcgttggc
      721 ggcgtccagg cccggcagcc ccgagccctg ctggacgatc accgctccgc ggccgacctt
      781 ccagtccggg tcctccgtgg cgggcttggc gccgccccag ccgaaggcct tccgcccgcg
      841 tccgatcatc tcccccaggc cgcattcccc cagggtgacc gcggcgccgg gccggccctc
      901 ccccaggctc cgcaggatct cgatgcggct cccggagcgg acgcggttgc gctcgatcag
      961 gtccagctgg tccatgccga gttcggcggc cagctccgcc agggcggtct ggagggcgaa
     1021 ggagcccttg ggggccccgt agccctggta ggcccccgcg ggggtgaggt tggtgtagta
     1081 gctcttcacc tcgaagcggg cgttgtcgca gagcaccagg ggcaggctct tggacacggc
     1141 gttcatgggc accgtgaggc agtgctggcc gtagggtccc gtgtccgcgt ccaccttcat
     1201 gagcaccgcg gtgagccggc cgtccttctt ggcgcccagc ttgacggtga tctccatcag
     1261 gtggcgggtc cggctggaga tgaactcctg ctcgcggtcg tagcggaaga agacgggccg
     1321 tcccgtggtc caggccaggt agccggccac gtcctccagg acgatgtcct gcttagcccc
     1381 gaagcccccg cccacccgct ccttgatcac ccggaccttg ttctcgggga tcccgacggc
     1441 gcgggagacg atgcggcgca cgtgccaggg cacctgggtg gaggcgtgga ggaccaggcg
     1501 gcccccgtcg accttggcgt agaccacgtg gggctccatc ggggtgcact gcacccggtt
     1561 gcccttgtag gtccgctcga cgaccacgtc cgcctcggcg aagcccttct cgacgtcccc
     1621 gatcccgtcg gccacgctgg ccgccaggtt gcggtgcggg tccgcgtgca ggggaaactg
     1681 gtagacgacg gggtcgtccc ggccgtcccc ggcggtggag gtacccgcgg gggcttcccc
     1741 ggaaacgtac cggacctccc cggagtggac caccggggcg cccggggtcc gggcctcggc
     1801 gatggagaac acgggcttca agacctcgta ctccaccttc aaagccttga gggcggtccg
     1861 ggcctggtcg ggggtctccg ccaggacggc cgcgacgcgg tcccccacgt ggcggacctt
     1921 ccgggagaac atcctctggt cgtgggggga gggttccggg aagccctggc ccgcggtggt
     1981 gtagacccgg tccgggcagt tctcgtgggt gaggaccgcc acgaccccgg gcaactccag
     2041 ggccgcggac gcgtcgatgc tcttgatcca ggcgtgggcg tggggactgg tgagcagggc
     2101 caggtggcag gcgtccgagt cgacccggtc ctccacgaag gccttctccc cccgggccag
     2161 ggcggcggcg tccaccttct cccggtcctt gcccacgtgg cgcaggtccg gccggaactc
     2221 cggggccgcg gcctccgcgt agcccgggtc cttgaggcgt ttcgcggcga tccgcacggc
     2281 gttgtggaac tgctcgtacc cggtgcagcg gcagagggtg cccgagaggg cgtccttgac
     2341 ctcctgcttg gtgggctccg gggagcgctc caggagggag tgcaccgcca ggaccaccgc
     2401 gggggtgcag tagccgcact ggacgcaggc cgcgtcgatc agggccctct gcaccaccga
     2461 aagcttccga tctttggaga agtgttcgac cgtgtagatc tcccggccct ccgcctgggc
     2521 ggccaggagg gtgcaggagt tcacggtgcg gccgtccagg aggacggcgc acaggccgca
     2581 ggagccttcc ccgtcgcagc cgtcccgcac ggagacgatc cccttgcggc gcagggcggc
     2641 cttgaggctc tccccctcgg ggcagtccca ggagacgggg cggccgttga gcttgaaggt
     2701 gacggtcatc ggcttacctc ccgggccgtg agcagggcct cggctacgag ttccgaggcg
     2761 cgcaggcgct tgaaggccgc gctcccccgg aggtcgtcga tcggcttgag cgcgggcgcg
     2821 acggccgcag ccgcctcgtc ccgcccgggc aggggccgcc cctcgaagag gcgttccagc
     2881 tcgggaaagc ggcggctccg ggcgtccagg cccccgcagg cgatacgtac gtctcgcaag
     2941 ctaccgtcct gcagacggta gcttgctgcg caggtcagga ccgagaggtc gcaggccgtc
     3001 cgggaccagc gggcgtaggc cgcccggagc cccggcgagg cggggatctc gatccaggtc
     3061 acgatgcccg agggcgcggc cagccagtcc gccaggggcc gggtttccgc cgcgccgtgg
     3121 atccgcaccc gggcgtccag cacgaggagg gccggaacca gggaggaaca ggagcgggcg
     3181 gcccccaggt tgcccccgac ggtggcggcg ttccggacgt tccggttggc catgcccagg
     3241 gccgcctccg agaggatgcg ggacgggacg gggctgtccg ccaggtcctg gaagaccgcc
     3301 ccggcgccga tgcgcgttag gccctcggcc gtccgctcga ggccccgggg caggagggac
     3361 ccggcgtcga cggccacgaa ctcccggtcc cggaactcgc tggtcaggag ctgggtgccc
     3421 cccgccagga tccgcgcccg cttgtccgac gcgaggaccc ggtcggcctc gtccagcgag
     3481 gccgcacgta tgtatttcac gaccatccgg ccacccccgc tttcgcgtag agcctggctg
     3541 cgatacggtt ggcgttgtcc cgtatctcct cctcgtcctc cccgaccagc cggccctgga
     3601 ccaccgcggc cttgccgttc acgaagacgt agtccgcccc gtggtcgtag cccgagaaga
     3661 ggagggccgc cgccgggtcc gagagggccc cggcgtattc catcttttgc acgtcgaaga
     3721 gggcgatgtc cgccagccag ccttcctcga tccggcccag cctgtcccag cccaggatcc
     3781 gggccccgcc ctcggtggcg atgccgtaga cctccggcgc ggtgatgccc gcggagccgt
     3841 agcgcacccg ctggagcagg agggcctgcc gcatttcacc cagcatgtcc gaggcgtcgt
     3901 tggacgccga gccgtccacg gccaggccca cgttcacgcc gcggtccagc atctcccgga
     3961 cccggcagat cccgctggcc agacgcatgt tcgaggaggg gcagtgggct accccggtcc
     4021 cggtccgcgc cagcacgtcg agttcctggt cgttgaaatg gatcccgtgg gcgtaccaga
     4081 cgtcggggcc caggaagccg cagtcctcca tgaccttgag gggacggcgc ttgtagatct
     4141 tcacgcagta gtcgtcctcg tccccggtct ccgccaggtg ggtgtgcaac cgggcgccgt
     4201 acttccgggc caggaccgcg gagtccttca tgagctgctc cgacaccgag aaggggctgc
     4261 agggggccag ggccacccgg cgcatggagt ccggggcggg gtcgtggtac ttccggatcg
     4321 tctcctcgca ctgggccagg atcgtgtcct cgtcctggac caccgagtcc gggggcaggc
     4381 ccccgtcctt cttggaccgg ctcatggagc cccgggtggg tgcgaatcgc accccgatgt
     4441 cgctggcggc ttggaactgg atgcccggca ggtcctggga aaagtccctg gggtagaggt
     4501 agtggtggtc cgtggtgcag gtgcagcccg tcttcacgag ctccgccagg gccaggcggc
     4561 tggaccagta caccgcctcc tcgtcgatcc ccttccagac ctcgtagagg tacacgagcc
     4621 actcgaagag cttggcgttc tgcaccgccg ggaggttccg ggtcagggtc tggtagaagt
     4681 ggtggtgggt gttcacgaag cccggggtca ccacgtggcg ggaggcgtcg atcacccggg
     4741 ttccctcggg cacgggcagg ttcttcccca cccggacgat ccggttgccg tcgatgtgga
     4801 cgtccgcccc cgccaggttg ccgccggggc ttcccggcgg gcgaacgatg caaagacagt
     4861 ccttgaggac gatcaccgaa ggacctcctt gttgaagtcc tcgatcaggg cgtccagggc
     4921 gccgacctcg ccgtggagct cgccccagta gccgcgccag tcgtcccact gggcgttcag
     4981 ttcggccagg tccttcccga gctgctcgat ccaggtgtag tacttgagct ggtggacccg
     5041 gcggcggtcc gtgtgggtca tgtccaggac gtagtccacc gagaggctct taagcatctc
     5101 gaggtcccgg tccgcctggc gctggtcgta ggggccccgc tccgcggcga gctccttgag
     5161 ccgggagccg tagagctgga gggagtccgt gaagaccgtg agggcgatct cgtccgcgcc
     5221 cagctcgtag tacttggcgg ccttgatggt ggccacgagg tttccgatcc cggagatccc
     5281 cagaaggtcg agccgatcca cggcggcctg gggcacaccg gcctccacga gggccctgcg
     5341 gcccgcaggc tcgttgaaca ggcgcagaag tcggacggtg atctcgtcgt ccacccccac
     5401 cgccacgtcg gtgtcccgga ggttgtggat ccaggggatg tgcttgtccc cgatcccctc
     5461 gatccggtgg cccccgaagc cgttctccag gatggtgggg cactgcaggg cctcgcccac
     5521 gcccacccgg gcaccgttgt gcttctcctt caggtaggag cccgcgccca gggtgcccgc
     5581 ggagccggag ctgtacaggg agagggccag gcgggacttt ccgtcggcgt tcatctttcc
     5641 gtagacctcc tccagggcgg gacccgtcac ggtgtagtgc cagaggtggt tgggcagctg
     5701 gtcgaactgg ttgaagatca ccgcgtccgg ccgggtcttc tcgatctcga cgcacttgtc
     5761 gaagatctcc ttcacgttgc tctcgcagcc cggggtggcg atgacttcct cggccatggt
     5821 cgccagccac tcgaagcgct cccgggacat ctcggccggt aggatggcca cggaggggca
     5881 ggacaggagg cgggagatgt aggccccgcc ccggcagtag ttgcccgtgg agggccacac
     5941 ggccttcttg gtggtggggt cgaattgccc ggtcacgagc ccggggacca ggcaggagta
     6001 ggccgcgccg accttgtggg cacccgtggg gaaccacttc ccgaggaccg ctccgatccg
     6061 ggctttggtg cccgtgagct cccgggggag ctcgaagtag ttcacgtccc cgaagactcc
     6121 gcccttctcc ttgggctcgt tgtgccagtt gacccggaag aggttgcggg agtggacatc
     6181 ccagaggccg atacccttga ggtcggcctt gatggaatcc gggatcttct tgggatcccg
     6241 catctgggcg taggtcggca ggaggatgcc cttttccttg caccgctgta tgttcttctt
     6301 ccgctgggct tcgttgatgg aaagatcgat cttcatgggt agctcctttt taggtgaatt
     6361 ggtgaatcgt gaatgagtga ataggaccgg atggcaggtt tacgagcgcg ttcggcacgc
     6421 atacccgctg tttccgcaat cctccctatt tacccattca cctattcact aattaactta
     6481 tccctaatcc gtgggccgcg ctcttcacgt ccttgagtcc gctgcccgtg acgaggagga
     6541 ccaccgtcga cgacgggtcc agctcgccct ttaccttcaa gaatcccgcc gccgcggcgc
     6601 tggaggaggg ctcggcgaag aggccggtcc cgctggccag ggtccgctgg gcggacagga
     6661 tctcctcgtc cgtgaccacc accgcccggc cgccgtactt tgtcagcttg gccagggcca
     6721 gggtcccgtt ccgaggcacg tccaccgaga tggagtccgc gatcgtggcc gagggctcgg
     6781 ggtccccgaa acctcccgca gacagggccc gggctatcgc gctggacccc ttggcctggc
     6841 aggcccagac cacgggcacc ttggaaaggc ggcccaggcg gactaagtcc tcgaagccct
     6901 tgtagacccc cgccaggatc accccgtccc cgacgggcac gaagacgtgg tccggcgccg
     6961 gggctccccg ggccgcgagg tccgagagga tctcgaagga gaccgtcttc ttgccctcga
     7021 tggtcagggg attgtaggcg gtgttccggg acagggtccc cgtggccttg gtgtaggcca
     7081 gggactcgtc gaaggccttg tcgtaggtgc cgtccaccgt cttgagttcc gccccgtact
     7141 gtaggacctg gatccgcttg gcgacgggag ccgccttggg caggaagacc gtgatcttca
     7201 agcccgccgc cgccccgatg cccgccatgg aggaggccgc gttgcccgtg gatgccaggg
     7261 ctatctcccg gatgcccttc ttgcgggcgt aggcggccac caggaagctg gcccggtcct
     7321 tgaaggagcc cgtgggctgg caggtgtcgt ccttgatcca gagacctttg aaaccggtgt
     7381 ccgcgcgcag ccgctccggc gcccacaggg gggtgtcccc cacgggcacc ggggggaacc
     7441 actcgggctc cacggggagc ggcaccgagc gggaatcccc ctcccacacg ccggcccagg
     7501 cgcactccag caccccctcc aggggtcggt ccgggagcgt cttgggggcg caggaggagc
     7561 agaggtagcg gtccggttcc atctcgtact cggcgccgca ggtagggcac cggtagaacc
     7621 atcgcatgcc gctcctcctt attagagctt cccttccaga gcgtagggca gggtagcgta
     7681 gaaggccgct gccttctcca ggtcgtccac ccgggtgatc tcgttggggg cgtgggcctg
     7741 gttctcgtcc ccgggtccga agccgatggt cgggatcttg tggcgcccgc acacggccac
     7801 cccgttcgtg gagaaggtcc acttgtcgac cacgggctcg gaaccccaga gggctttgtg
     7861 ggcggccacc ccggcccgga ccagggcgtg gtccgcgtcg atcttccagg tcgggaagta
     7921 gaggtcctgg ccgtacttgg tacccttcca ggaggggatc tcgtagtagg ggaccttgac
     7981 ctcgaagttg gacagtccgg cctcggagag ggccttcttg acctgctcca ccgccagctc
     8041 cttggtctcc ccccaggtga ggcggcggtc caggtagatc atcccctggt ccgggacggc
     8101 gcagagggag ggacccttca cgtccatgcg gctgaccgtg acggtgccct tgcccaggaa
     8161 attgtccgtg tcgggcttca ggttctcgtt gagcttctcg atcgccaggg ccgcccggga
     8221 ggccatgtag gccgcgctct tgccgcgctc cggggcggag ccgtggcagg acacgccctg
     8281 gaagcggcac tcgatctcca tgcggccccg gtggccgcgg tacaggcgga gggaggtggg
     8341 ctcggtggac acgaagaggt ccggtacgag cttttcctcc tcgatcaggt acttccagca
     8401 caggccgtcg cagtcctcct ccatgatggt gaaggcgaac cagaccgtat agtccccggc
     8461 gtagccgagt tccttgagga tgcgccccgc ggtgatcatg gaagccgccc cgcccagctg
     8521 gtccgaggcg ccccggccgt gcacgaggcc gtcggccacg aggcccgaga agggcgggtt
     8581 cttccactgg gccgggtcgc cggtgtccac ggtgtcgatg tgggcgtcga cggccaggat
     8641 cttctttccc gagcccaccc gggccaggag gttccccagg ccgtccacct tgacctcgtc
     8701 gaagccggcc ttctcgcaca tcgacttgag cagggcgatg cgctccttct ccttggcgct
     8761 cagggccgga acctggatga tccgggacag gatgtccgcg gtctcgtccc ggtaggacga
     8821 ggccttcgcg cggacctttt gggcgttcga gtccatacaa cccccatgtc ctgccccgta
     8881 cggcccgagt cgccgcgcgg ggggaagaaa actaaagtcc gttcccgccc gaatcgcggg
     8941 gacggacaat ccgcgtcggc gggagagggc ccacggccgt ccctccgtac gcggaacgaa
     9001 attctacaag gtatccatcc gcttccagag ccgggcggcc tggacgcggc cgtccgcggc
     9061 gatccccgcc gcgtcgaagg cgggcgcccg gtcccggacg acgaaggccc cgtcggaaag
     9121 gacggttcgc acgctccggc tggagagccc gaaggccagg tggcccgcca ggttctccgc
     9181 ggacaggggc gtgggcggat cgtagtccca gtggaccagg tccgcgggat acccgggctc
     9241 cacccggccg aagcgcgttc cagccatgta tcgttccatg agacggttgc cccggtcaag
     9301 gacccccagg aagtccccgg gccaccaggg ccccccggcc tcccggtggc ggaagtaggc
     9361 gaacttgaac tcctccgcca tgtccgcccc cataccgtcg gtccccagga cgggattttt
     9421 caggccgggc aggagggacg cgtagcccac ggcgttgttc atgttgctcc gggcgttgtg
     9481 ggccaggaag cagtcccgct cgttcaggag ctccacctcc gagggggaga gccacaggcc
     9541 gtggcccacg atggtcttcg gccccaggag cccgaacttc tccatccgtt cgaccaggtc
     9601 cgccccgtag cggtgccggg agtccgaggc gtcgtacctg tcctccgcgg cgtggacgtg
     9661 gaagccccgg cccgtggacc ggaccgcctg ggcggagagc tccagggtct cgtcccccat
     9721 ggtgaagagg gcgtggcctc ctatggcggc ctccacccgg ggcgccttcc cggccttccg
     9781 ctcggcgtcc aggacgcggg cgaagcgctc gttctcccgg actcccaccc gggccccctc
     9841 cagcccgttc cggtccgtgg tctcgtagca caccaggccc cgaagtccga tctcgtcgta
     9901 ggcggcccgg atcacgtcca ggctcccgtc gatgaagccc ggggaggcgt ggtggtccac
     9961 cacggaggtg accccgcagg ccgccgcgtc cgccagcccc gcccgggcgc tggcccggag
    10021 gatcggctcg tcgatggccc ggtccaggcg ccaccagagg tgggccagga tctgcccgaa
    10081 gtccttggag ggccggatgt tggccaggat gccccgggcc agggcggagt agaggtgggt
    10141 atgggagcag acgaggcccg gggaaaggta gcccccggag cccgcggccc gcgcggcggg
    10201 gtacttggcc ttcaggttcg ccccggcctc cgccaccgtc ccgccctcgg cggcctcccg
    10261 gaggaccacg tcccgaggtt ccgagaccgc ggcggggttc caggtgatga ctcggacgtt
    10321 ctcgaagatc agcatgcgtc gcctcccgcg aggtagggat gttcccggcc cacgatccgg
    10381 gccagggcgg ccatccgggc cgccgctcct gcgggccctg ccgcgccggg cgccgaagcc
    10441 gccgatgcta cggcccactc ggcgtggggc agggtccgga cctcaccctc gtactccgcc
    10501 cggaggacca ggtccggggc cccggccttc ccggtgaagg cgaagcccgc gttccgggag
    10561 gcttccaggg ccccggcgga cgcgaagacc gtgggcttgc cgcggtaggg ctccccccgc
    10621 caggggcaga agaatccgca gttgccgcac tcgttgcaca gggcgtccac gtggaggatc
    10681 tgtaggcttt gtgtgaaacc ggtgccggtc ggcacggcca cgaaggtgtt ggcccggttg
    10741 gggcagacct ccacgcagcg caggcaggcc gcgtcgcact ccaggcagcg ttccgcctgg
    10801 gcggccgtga aggctctggc accggcaccc tcgggcaccc ggagctcctc ccgtgccgcg
    10861 gcggccggat cctgggagcc ccggcgggcc agcttgtccc gatccggggc ggggggggcg
    10921 taggatcccc aggcgccctc gggctcgatc cccgcggccc ggaggatggc cgcggcgctg
    10981 cgccggccgt ccgccacggc ggagatgatg gaggccgggc cccggagggc gtccccgccg
    11041 acgtagaggc ccgggacgcc gacggaaccg gtctcgaggt ttgccttcgg gcggccgtcc
    11101 ttaccgaccg gtacgcccag ggcctccagg aaggcgcggt ccggggtctc cccgaccgcg
    11161 gccaccacca ggtcgcaggg cacgtccacg gtctcgccgg agggaaccgg ggagcggcgg
    11221 ccggaagcgt cccgctctcc cagggtcata cgccgcaggg tgagcgtgcc gggagcggcg
    11281 gactccggca gggtcagttc catcagggcc gtcgggggcg acgcggcgcc cgacgcggag
    11341 ttaaccgcgg aggcctcgga cagggcgttg tggagctcct cccggtccgc gggcatctcc
    11401 cggacggtgc ggcggtagga aagccggact tcccggatcc cggggatgcg ggaggccgcg
    11461 cggaccgcgt ccatggcggt gttaccgccc ccggccacca cgacgtgccg gacggccgcg
    11521 aagggacccg ggccctcggc ggtctcggcc aggaactcca gggcgtccac gacgcgaact
    11581 ccggagccgg taagggggag atcccggggg gccggggagc ccgcgcccac gaagacggcc
    11641 gaatagccgt ccgcccgcag ggacccgaga tcccggacct cggcgccgaa gcggaactcc
    11701 acgcccatgg accggatgcg ctcgatgtcc cgctcgatat ccttccgggg gatccggaag
    11761 ggggggatca cgttggcggg gacgcccccg ggggaggcgg ccttctcgaa gaccgtgacc
    11821 ggaacccctt cccgggccag ccagtacgcg caggccagtc ccgcgggtcc ggcgccgatc
    11881 accgcggctt tccccgggaa atctttccgc cgggcccggg gtttgggcgg aatctccgcg
    11941 gcccgggcgc aggccagctt gaccccccgg atctccacgg ggccttcgta gtcgttccgg
    12001 cagcaggcct cctggcagac gtggtcgcag agggtgccgg tgatgtacgg aagggggttg
    12061 tccgccagga tcagggccag ggcctcctcg tcctttcctc gagccgagag gcggatgtac
    12121 tcggggacct tctggttcac cggacaggcc cggacgcagg gggcgtcgaa gcagtcgaac
    12181 tccggcaggg gcgcgtcgat ggccgcctcg ccctccttcc agtccccccg gaactccggg
    12241 cgccccaggg ccgcctccgc cagggccgcc agggaccggg cgtccggacc cttcggggcg
    12301 gcgggcaggg ccgagacggc cgaacgggcc atatccgcca ggcggaggta gccgccgggc
    12361 ttgaggatgt ccgtggccac ggtcaggggt cccaggcccg cccggacgca gtccgcggcg
    12421 ttcagggcgg acacgccccc gcagaaggag aagggcaggg gcccggggac cgcggcggcc
    12481 aggtcggcgg ccaggcggac cgtgatcggg aagagggccc ggccggacat gtagcgctcc
    12541 tggccgggga ggcggtcgcc cacgttcacg ttggccaggg tgttcgagag cttgaggccg
    12601 aagcgcaccc ccttccgttt ggccgcggcg gccagggagg tgatcagggc cagggcgtcc
    12661 ggccactgca ggtcgtgctc gaagcccggg cgctgtattt ctacgtccgt ccacccggtc
    12721 cggtccagga tctcccggac ccggtcgtag cccaggaggg tggggttcag cttgatgtag
    12781 gtggagaagc ccttctcctc gatgaggtag cggccgatcc gctcgatctc cgcgggggga
    12841 cagccgtgca tggtggacag ggtcaccgag tggaccggcg cggccggcat ggaatcgacc
    12901 gccgcggcag cccgggcggc cccctcggcc ccgaaggcct ccctaaaggc gggggaggtc
    12961 accgcggccc ggagctccgc cagggcggcg ccccagaagg gggtggaggc gggggcccgc
    13021 atcccctcga tgaaggcgtc catggtgggg ctctttatac cctccagggt gtagcccacg
    13081 gacatgttga agaggaaatc cccggccccc ctctccgcga agagggcctt gagcacgtgg
    13141 agggccataa atccccgcag gtactcatcc cgggcctgga ccagcgagag ctccgtggac
    13201 cactccacgt tgtgcccctc gtccagggcg tcgatacagg gcttggcgat ctcgagggcg
    13261 tcgttcacct ggacggtctt gagctcgaag atccgggagc ctcccaggta ggaggcgacc
    13321 aggttcgggg cgatctgggt gtggggcccc gccgcggggc cgatcgggta gaggatgcgg
    13381 cttcccagga cctcgaagcc cggggaggcc ggctcgtcct cgaagacctt gcggaagagg
    13441 gcttccggga ggtcgtagac gctctttttg gccttgtact ccccggccat ccgtccgatc
    13501 agcgccccca tgggcgtggt ttccataacc ttgctcgtgc tcaccggtac ccccaacgca
    13561 ggaatctgcc gctccccgga gcggcgcaaa tcggatgctc gtttcggtcc gcccgggccg
    13621 cgtcgtacac gaggcgtccc cggacgtagg tcgcgaggaa gcggccggag aggcgcatcc
    13681 cctcgaaggc cgtaatcttc cccttggaca ggagggtccg gccctcgacg accgtggtcc
    13741 cccggggatc cgccaggatg aagtccgcgt ccttcccggc ctccagggaa cccttgcggc
    13801 cggaaaggcc gtaccgctcc gcggcggccc gggcggtcac gtccaggtag cggctcaagg
    13861 agaggcgccc gcccaggagc ccctcggagt agaggaaggg ggcccaggtc ccggtcccgg
    13921 ggatcccccc gtagtcggtc cagaggtctc cggtgttctt ctcctcctcc ggggccgggg
    13981 cgtggtcgct ggtcacgaag gagatggccc cgcccgaaag gagggcccag agccgctcgg
    14041 cctggcccgg ggccttgacg ggcggggcgg tcttgaggga ggagcctagg ctctcgaagt
    14101 cctcggcgga gaaggccagg tagtgggcgc aggtctcgca ggtggccccg gacgcggcca
    14161 ggagctccgc ggcctcggcg gtgcccacgt ggaccacgtg gagccaggcc tcgttccccc
    14221 gggccagggt cgcggcggag gcggcggcca cggtctccgc atcctcggac cgggcgccca
    14281 cgtagtcgct ccaccggggg gcctccgcgc cccgggcctt cctcaggcgg gcctccgcgg
    14341 cggtaacgca atccaggtcc tcggcgtgca ggaggatcgg tcgtcccagg gccgccccgc
    14401 ggcggacgat ccgcccgaag tcgtcgtggg tcacccgagt gaaggtcttc atcccggaga
    14461 tgaagtaggt cttgaagccc accacctccg gggccagggc ctccatgtgc tccgataggc
    14521 tttcctccac ggtatggccg gagacccccc cgaagaaggc gtagtccacc accgcggcct
    14581 tggacacgaa ggagagtttg ttctccaggg ccgcccgggt ggtgaccggg ggcagggagg
    14641 tgcagggcat gtccaccacc gtggtcaccc ctcccttggc cgcctcggag gtgccgtgga
    14701 ggaagtcctc gcggtgggtg aagcccggct cgtcgaagtg gacgtgggga tcgatggccc
    14761 cgggcaggac ctgaaggccc cgggcgtcca ggacctcctc cccgggctgg ggggtcaggg
    14821 tctcggcgat ttccgtcacg cgcccgtccg ggcctacccg gaagtcccgg cgcaggaagt
    14881 cccgctcgta gcgcagggcc aggagtccgt tcttcacgat catgggatca cccccgtaag
    14941 gtagtccagg gccttttccg gcgtgagggg caggtcgtgg atgcccgggg cccggaggcg
    15001 gtccccccgg gcggccttga gggcgtccag gaccgcgaag tacccggcga tgccgtacat
    15061 gaagggaggc tccccgatgg ccttgctttt cagcaccgcc ctggggttcg gggcgtcccc
    15121 caggaactcc aggtccacgg gaaagtccag gaagtgggcg tccgggagct tgtacgtgga
    15181 cagggtgtcc gagaggagcc tcccggtctc gtcgtagcgc aggtcctcca ggagggccca
    15241 ccccaggccc tgggccaggg ctccctcggt ctggccccgg tccacgacct cgtcgatcac
    15301 gttccccgcg tcgtggacca gggtggcgga tgcgatccgg taggtgcccc ggaccgtgtc
    15361 cacctccgcg atgaggaccg cgcagccgta gacgtggtag gcgaaggggc tgccccgctc
    15421 cgccttgccg tcgtagaaga gcccgggggt cgcgtagaag ccgtgggccg agaggtctat
    15481 ccgggcctcg tgggccttgg ccacgagggt ctcccagggg gcgtccgcca ggcccgaccc
    15541 ggagcggaag gcggaaaggc gcttcccgat ctcctcgcag gcgaccaggg ccgccatgcc
    15601 gttcaggtcc gtgcccgtgc tggccgcggt ggggctcgtg ttggccaccg cggcggtccg
    15661 ggtgcgctcg atccggaccc ggccttcggg gatacccagg gtcagggcca cgaccttctg
    15721 gatcttccgg ttgaccccct ggcccatctc cacggccccg gtgtggaccc ccacggaccc
    15781 gtcccggtag acgtggacca gggcgccgcc ctggttcatc atgagcttgg tgaaggacac
    15841 cccgaagcag agggggaggg cggccatgcc cctcttccgg tcccgatggg cgcggttgaa
    15901 ctcgtcgatg tccgcgcgcc tgcggtccca gccggaccgc tggagggccc ggtccacgct
    15961 ccgctccgtc cgggcctccg ccacgggcat gccgtagtgg aaggtgtccc cggtgcggag
    16021 gaggttcttt ctctgcagtt cgatcgggtc cacgcccatc gtctccgccg cccggtggag
    16081 cgccgcctcc atcacgaaga tcccctgggg ggcgccgaag ccccggaagg ccgtgaaggg
    16141 gggcagattg gtccggcaca tccagcccgt ggcccggagg ttggggatgc ggtaggcgct
    16201 cgcggcgtgg aagagggtcc gggaaaggat ggccggggag aggtccgtgc aggccccgga
    16261 gttctggtag tagtccacct cgaaggccag gatccggccc tccgaatcca atcccagcct
    16321 gtagtccgag ctgtagggat gccgcttccc ggtgaaggtc aggtcgtcct tccggttcag
    16381 gtagaccttg accggccggc ccgtgacgta ggcccccagg gcggccaggg cggaccagga
    16441 ggcggcctgg tcctccttgc ccccgaaggc cccgcccagg cggggggcgt ccacctccac
    16501 ggcgttcatg gggagatgaa ggttgcgggc caccgcctcc tggacccccg tgggggactg
    16561 ggttccggag aggacgaaga tcccgccccc gtcccggacc tgggccaccg ccccctgggt
    16621 ctccaggtag acgtgctcct ggcccccgga ttccacccgg cccgagacga ccacggcgca
    16681 gctcctgaag gccgcctcgg ggtcccccgt gcgcatggtc cggggggcca ggatgatcat
    16741 cccccgggcg tgggcctccc ggggatccgt gaccgccggg agctcctcgc cttcgatttt
    16801 gaccagggcc gccgcacggc gcgccaggga ccgggtatcc gccaggacca gggccaggga
    16861 ctggccccgg taggtccact cgtccgcgac caggaggggc tcgtcggaca ccgcggtgcc
    16921 cagttcgttc tccccgggga tgtcccgggc gctcaggacc cggacgctgg ggtggagggc
    16981 gagggccgcc gaggggtcca gggaacggag ccgcccgtgg gcggacttcg acaccacggg
    17041 ctggccgtgg aggaggcccg cgggctcggg cagatcgtcg atgtacttcg tgcggcccag
    17101 gacgttctgg accgactcgg gattccagga tttcatggcc agaactcctc cagaagaccc
    17161 ttgtccggga agagccggac gaaatgggcc aggaccaggc gctccaggag gcgcgtccgg
    17221 tagcccgcgg aaccccgcac gtccgagagg ggcgagcagt cctccaaggc cagccgtgcg
    17281 gtttcccggg cggccgcggc gtccggcgac ttcccgacca ggaaggcgga ggccttccgg
    17341 gccaggaggg gcaccgcggc catcccgccc accgagaggc gggcccaggc gatccggtcc
    17401 ccctccatcc gcagggccag ggccgtgttg gccgtggcga tgtccaggct ttcccgcttg
    17461 gagaccttct cgaagttcag ccggaccggt cccgccgccg cgggcagaag gagggtctcc
    17521 aggaattccc cgtccctcag ggcggtcttc ttgtagccca ggaagaagtc cgccagggac
    17581 agttcccggg cccccgaggc ggagcggagg cggacccggg cgtccagggc caaaaggagg
    17641 gcggtcatgt ccgcgatggg ggaggcgttc accacgttcc ccgccagggt ggcgcggttc
    17701 cggatgggga ggctggccat ctgggcctcg aaggcctcga tccccggggc gtgggcccgg
    17761 atccgggggt cctcgaagaa ggagcgcacc gtgacggcgg ccccgacgga gaggaagtcc
    17821 ccggtccgct cgatccgatc caggcccgcc tggcgatcgg tgaaggcgac tccggtcccg
    17881 ggttcggggt cgggattgcg gacgaaccag tccgtgcccc cgcccaggtt gagtcccttc
    17941 ggggcggcgg cgggccggct gcccggctcg ggaaggctcg tcatgagggc ggccagggac
    18001 ccgggcagaa ccttccggtc ccccaggacc gccagccgtg ccccgaggtc cgcgggcagg
    18061 tccgcgaagt cccgggtcag gcgctccgcg gcccgccgga tggacccgta gcccgtgcag
    18121 cggcagaggt tcccttctat ggaaaccagg gcccgcaccg ggtcgatgtg tccttccacc
    18181 aggaaggcgg tcagggccac gatgaagccc ggggagcaga acccgcactg gctggccccc
    18241 tcgtccagga aggcctccat cacgggggtg aggccccccg cggcgatgcc ttcgatggtg
    18301 accacgtggc gcccgtccag ctcccccagg gccaggaggc aggagggcac ggccttccag
    18361 gacggtccgt ccccgccccg ttcccccagg aggaccaggc aggccccgca gtcgccctcc
    18421 cggcaccctt ccttcgtacc cgtgagtccc aaatcccggc gcagatagtc cagcgccagg
    18481 gtctcctccc aagcttccgc gtccacgagg cgttcgttca cgcggaagac caatcgtcgt
    18541 ttcatccggc cttcctctta tagcttatta taatggtaat ataggatttg tacaccgaaa
    18601 cctgccgggg aaaccacccc gggggcggaa cgctcctgta cgtgggacat gacccggatc
    18661 agtgtacacc aggattcggg gaccggcaac tcgaaggaga acgtcggctt gattctccgg
    18721 aacggatcgg caacactggg tacgggttcc ggcatcccgt ccggagcccc accccggacg
    18781 cccgcgtccg catccaggag atccgccatg ctcagaagtc ccttcctcct gctcggcctg
    18841 gtctggatgg gggcggccgc ggccgcccag ggggtccccg ccgcggcctc gaccggaacc
    18901 cgcgccgagc tccgggaatg gcgctacgtc cccggggatc gaccggagta cgcggatccg
    18961 gggtacgacg actcgggctg ggcatccgtc cgcctgcccg cccgcctaga cctggacgcg
    19021 ggccggggct ggctccgcgg ggagttcgac ggaagcggga tgccggacgg cgaggtgtac
    19081 ctcctgctgg gccgggtgga cgcggccgcg gaggtctacc tgaacggcag cttgatcggc
    19141 cggcgcggga ccctggtccc cgagttcacg gtcccgggca accagccggg ggtcttcctc
    19201 ctgccttccc ggctctggaa cccccggggc ggaaacctcc tggccctgcg cctggccgcc
    19261 ccggcctccg ccctggacgc gtcggggttc cagatcgggg acggctcggc cgcggactac
    19321 gagcgccggg tcatcggatt cttcaacttc gagctgtaca ccgtgctcgg ggccctctgc
    19381 gccttcatcg gcctctattt cctggccctc tgggcgggac gtccagcgga cacccccaac
    19441 ctctggtacg cggtctcctc cttcgccatc gccttctact tcgccgagat cggggcgacc
    19501 tacccgatcc tggagtacac cctgaaccgg gccctggcca aggcctgcct taccgtttcg
    19561 atggccgccc tggtcgcctt cttcctgagc ttcttccggc tccgcctgcc ccggatcctg
    19621 gtcgccctcc tggccctggt gcccgccgcc tcggtcgtat cctacctggt cgtccggggc
    19681 gactacgtcc gggtggacgc gatgttcaac cggaacctga tcttcgtgca ggcggccatc
    19741 gtcttcatcg tctttgtgac cgtccgggag gtgatccggg ggaaccggga ggcggtgccc
    19801 atcctggtcg gggtggtcct gggggtgggc ttcggcaccc acgacgtggt ctactcggcc
    19861 ctggggcgca agcccgtcgc ctggctccag ggaatcgggt tctttgccct gaacctctcc
    19921 ctcttcgtga ccctgaccct ccgctcctcc cggctccacg cggagctccg gcggtattcc
    19981 gcggacatcg agcggaagac gggccagctg gcggactaca tcggccggat cgaagagacc
    20041 gcggactcgg tctcggccat caccgcggag atcgattccg acgccgccaa ggcctcggag
    20101 tccgcggaca agctggccgc cgaggcgggg aggatcgggg cgaacgcgga gcgccaggcc
    20161 cgggccgccc gggaatccgg ccaggccgtg gagcgcctcg ggcggtccct ggggctcgtg
    20221 cgcacggggg tggactccca ggcggcgggg atcgagggct ccgcggaggc cgtggcggtc
    20281 gtggcggacg ccgccgcctc cgtcgcgggg agcgtatcgt ccaccgcgga cttcgcccgg
    20341 ggcctgggcc tgaccgtgga gagggggcgg gactcctcgc gcaccctggg ggaggcgatc
    20401 gaccggatca aggaagccgc gggccgggtg gtcgacatcg taggagcggt ggaggagttc
    20461 gcggagcgca cgaacctcct gtccatgaac gccgccatcg aggctgccca tgccggcgcg
    20521 gccggccggg gcttcgcggt gatcgcgggg gagatcaaga gcctggccgg ggcctccgcg
    20581 gagcgggccg cccggatccg ggactccatc gccgacatcg tccggcggat cgagaacggg
    20641 gtggaggcca acgcgggcct ggcggaatcc ctggattccg tggccgaggg ggcccggacg
    20701 gccctggagg ggatcctctc ggtctcgggc tccctggagg cccagcgcgg ggccacggac
    20761 cgcctgcgca cgagcctccg ggacctggcc gactcggcgg cctccatccg cgcggaggcg
    20821 gagcgtcagg acgaggaggg cgggcgcatc cgggagcgca tgtccgagct ggtgtcgatc
    20881 tccgacgagc tgggccagtc catcgggggc atagcccggg agaacgccga aatcgcggac
    20941 accatcaagc gcctggccga ggtctcccgg gacggaaagg aagccgtgac gggtctgcgg
    21001 cggctcctgg acgcgcggga gggacccgcc tccggctagt ccacgaagag gggcaccggg
    21061 gcgaagcgct ccacgtccac gagcccccgg tccgtgatct tgagcttcgg gatcaccggg
    21121 agggccatga aggagatggc catgaggggg gactcgttcg tgagcccctc ggcgcggaag
    21181 agctcgtcga aggcccgcag gcgctccacc acccacatcg cgtcccggtc gctcatgagc
    21241 cccgcgatgg gcaggggcag gtccaggacg atccggtccc cgtcggtcac gaccatgcct
    21301 ccgccgatct tgttgaggtg gcgggcggcc ttgaagatgg aggcgtcgtc cgcccccacc
    21361 aggatcatgt tgtgggagtc gtggctgatg gtggaaccga tggccccccg cttgagcccg
    21421 agccccgtga tgaagccctt tccgatgttc ccggagccct tgtgacgctc gatcacgaag
    21481 atcttgagga ggtcccggga cacgtcggac acgcagagcc cctcccggac cgggggctcg
    21541 gtctggatct ccccggtgat cagggtccgg ggccgggcct cgatgacgcg gatcctccgg
    21601 ccctccgcgg ggatgcggaa gtcctcctcg gtcagccact tgatgttcac cgagtcccgg
    21661 agcaccgcgg gccggttctc cacggcccgg accaggcgtc ccccctcggc cacgatctgc
    21721 ccggacttga tcaccacctc cgcctcgaag gcccggaggt ccgaggcggc cacgaagtcg
    21781 gccttgtagc ccggggcgat ggccccggag ttccgcagtc cgaagtagcg ggccgcgttc
    21841 agggaggaca tggcgatcgc gtccaggggg tccaccccgg cgtccacgag gagccggatg
    21901 gaccggtcga tgtgcccctc ctccgcgagg tccgtggggt ggcggtcgtc ggagcagagc
    21961 aggaaccggg gggccgtgcg gggggtgacg gccgggacca ggcgggccag gtcccgggtg
    22021 gcggagccct cccggatcat cacgtacatg cccttggaga tcttctccag ggcctcctcg
    22081 gcggtgacgc actcgtggtc ggaccggatg cccgcggccg cgtaggccga gaggtcccgg
    22141 ccccggagtt ccggggcgtg cccgtccacg gccttaccgg agtcccagaa gagggcgatc
    22201 ttgtcgagca gctggggatc cttggccagg acgcccggga agttcatcac ctcccccagg
    22261 cccaggaccc ggggttcccg caggaagggg tacatgtccg aggcgaagag ggccgccccg
    22321 gaggagtcga agtccgtggc gggcacgcag gagggggcca tgaggtagac gtccaggggc
    22381 agtccctcgc tggcccggag catgtaggtc atcccgtcgt acccgaggac gttggcgatc
    22441 tcgtgggggt ccgcgaccac cgcggtggtg ccccggggga ccacgaggcg ggcgtactcc
    22501 gagggcgaca gcagggagga ttctatgtgg acgtgggcgt ccacgagtcc ggggaccagg
    22561 acgcggccgt ccaggtccac ctcggtgcgg gcggcgaggc cgtccccgac ccccacgacg
    22621 tagcccttgt gcaccgagac cgtgagaccc gggcgcacct ccccgcgaaa gaggtccgcc
    22681 accgagcaat tgacgaagtt gacgtcgcag ggaacaaggc cgcgggccgc gtcgatctgc
    22741 tccttggaca ccatgcggta cctccgtgtt cgaagaatgt accgatcatt ctataccgcc
    22801 ggcccgcccg gcgcaaatcg ggccgcggcg ccggggatct cggactgccc gggttcggaa
    22861 aaagtgttgc gcgcccgccg actccgtcgt acactgtccc gtcgagaacg ccgggcttcg
    22921 cccggcgaca aaaacaggag gaagaagtgg agaaattctt caagctcaag gagcacggca
    22981 ccaccgtgcg cacggaagtg atggcgggca tcacgacctt catgaccatg gcgtacatcc
    23041 tggccgtgaa ccccggcatc ctttccgcgg cgggcatgcc cgcgggcggg gtcttcaccg
    23101 ccacggccct ggcgtccctg atcgccaccc tctgcatggc cctgctctcg aacctgcccg
    23161 tcgccctcgc gcccggcatg gggctcaacg ccttcttcgc cttcgcggtg gtcctgggga
    23221 tggggtattc ctggcagctg gccctgaccg ccgtgttcgt ggagggcgtg atcttcatcc
    23281 tgatgtccct gttcaacatc cgggaggcca tcgtccgctc gatcccggcg aacatcaaga
    23341 gcgccgtctc cgtgggcatc ggcctgttca tcgccttcat cggtctgcag aacgccgggc
    23401 tcgtggtgaa caacgacgcc accctggtgg gcctgggcaa catcaccaag ggagcccccc
    23461 tcctggccct catcggcctt gtgatcatgg gcgtgctcct gatcttcaag gtcaaaggcg
    23521 cactgctcat cggcatcctg gccacgacga tcatcggcat ccccttcggc gtgaccgtga
    23581 tccccaaggg ctggaacccc gtctccgccc cggctgcgcc cctcttgttc cagttccagt
    23641 tcgacaagtt cttcaccctg gacttctgga tcgtcctgtt caccttcctg ttcgtggaca
    23701 tcttcgacac tatcggcacc ctggtgggcg tgaccaccca ggccggtctg atcagcaaga
    23761 agggtgagat cccccgggtc aaggaagccc tcctgtccga cgccatcgga accgtggccg
    23821 gcgcctgcct gggcacctcc acggtcacct cctatatcga gagcgccgcg ggcgtggcgg
    23881 agggcggccg gacgggcctc acctccctga ccaccgccgt cctcttcgga gtggccctct
    23941 tcctgtcccc cctcttcctc ctggtacccg gcgcggccac ggccccggcc ctgatcctcg
    24001 tcggcctgtt catgctctcc cccgtgaagg acctggacct caaggacttc tccgaggcca
    24061 ttcccgcctt catcacgatg ctcttcatgc ccctgaccta cagcatcgcc gagggcctcg
    24121 tgttcggcat cctggcctac atcctcctga agctctgcac cggcaaggcc aaggagatcc
    24181 ccgtggtcac ctggatcgtc gggttcttct tcgtactgaa gcacgtgctc tgatccgcat
    24241 ttgcggcgcc cgggaatcag tcccggcgcg ccgcgcgttc cggaccgcgt ccacggaggc
    24301 agcgatgcga ctacggcttc ccttcgcgcc cgcttttctc gccgccctgg cggtcctgtc
    24361 ggccgccgcg cccgcggggg ctcagtccga cgtgagggtc cccgactgga tggcctacgt
    24421 ggagggagga acctacctcc agggttcccc ggatgccgaa gccgggcgct ccttcgacga
    24481 gggcccgcgg cgggaagtca acctgtccgc cttcctgctg gccaagaggg aagtcacctt
    24541 cgacgagtac gacgagtact gcgccgcctc cgggaaatcc cggccggacg acaagggttg
    24601 gggccgatgg tcccggcctg tgatcaacgt cacctggcgg gacgcggtgg aatactgcaa
    24661 ctggagatcc cggaaggacg gcctcgcgcc cgcctatgcc ctgaacggcg acaccgtcac
    24721 ctgcgactgg accgccgagg gataccgcct tcccacggag gctgaatggg agtacgcggc
    24781 ccggggcgga aagaggaccc gggggtttcc gtatcccggc ggaagcgacc cggaagccct
    24841 ggcctggttc tccctcaacg ccgggaaccg gacccatccc gcgggcggga aggccgccaa
    24901 cgaactcggc ctctttgaca tggccggaaa cgtctgggag tggtgctggg actggtacgc
    24961 ggggtatccg gccggcaagg cctccgaacc ccgggggccg gagggggccg ccctgaaggt
    25021 cgtccgcggg ggcggctact tcaacgtggc ctccaacctc cgcccggcca agcgctggtt
    25081 cgtggatccc gcccacaagt tcgactacct gggcttccgc ctggcgaggt ccctgccgcg
    25141 ccgctgacga caggacgaca tccacaggac ccaaccacgg agtacacgga gatcacggag
    25201 gatgagagaa tcacacaacg gattcgacag gatttgcagg attcgatacg gatgaacagg
    25261 attaagaact agagtagagg gcgataatca aattctaacg tccctttcct ctcatcctgt
    25321 acatcctttt tcatctcgtc catcctgtcc aggttcctca aaaaaacgcc gcccgggaaa
    25381 ggcggcgtta acagggtcgc ggccggtgtc ataacgtccg ctggacggtg gtcttggaga
    25441 cgtaggcctc cgcgcagtcc ggcagggccc ggacgcggtc cctccagccg atcgcttcct
    25501 tctccgaggt gtacggcccg atccgcaccc ggtagtgggt cgtcccgtcc acgtccttca
    25561 gggtgatcac cgaggacagt ccctgccggg ccaattccgc ctggaggctt tccgcccggg
    25621 agcggctggt ggggctggaa gcctggatcc accactccgt gacggtcacg gtcttgggct
    25681 tgggggcggg ggcggagggc ttggccgcgg gggccttggc ggccggggcg gccgcgggag
    25741 tataggagcc gctcttggcc ggcgcgggca gggcggtccc ggccggtgcc gtctgcgctg
    25801 cgcccgtggg agatcccggg gcggccgcgc cgggcagggc cgccgcggga ggattctcgc
    25861 cgtacacgat cacgacctcc ccgggggcgg ccggaagggg agcggcggga tcgggcgccg
    25921 gggcaggatt gcgcaggtac tcgtcgggct cgaccgcccg gggaggcgcc tcgttcccga
    25981 tcgccgcggg cgcctggggg gcccccttgc gaggggcgaa gagcaggagc gccgtcccga
    26041 agaacacgac caggaacaac ccgacggata tcacggtcca caggaccttc tttgtctcaa
    26101 ccggcatcgc aagcccctcc cgaacgttgg ggaaccggtc cgccctcgtg gaggctcgcg
    26161 gacgccggtt cctttctcct ttatcggcgg gaaggccggt ccggcttagc gcaaccgccc
    26221 gggtgtcacg aagcctgtta ggaggtcggc cggccccgcc cgaggcgctc cagggccgcg
    26281 tccagccggc gggccaggag ggcctccgac ccggagttcc gcaggaccag gaccgggccg
    26341 ggatactccc tcctccgccc ccagaggggc ctctggcggc ggatccggtc caggatggct
    26401 cccggcggaa gcccgtcccg ggctcggccc cggcggattc tagcgagcag gggggagcgg
    26461 acctccagga ccgccgtgca gcgtgcgacc tggggcagtt tgtagaggat ggcggcgttc
    26521 aggcagaggt cccggtcccc ggcatcccgc aggatctcgt ccaacagggc gaagagggcc
    26581 gggtggacca cggcctcgta gcgggccagg agggccggat cggcgaagac cctgcggccg
    26641 atctccctgc ggtcggggct gccgtcgggc cggaggatcc cggggccgag aagctccgcc
    26701 gtctccgcca ggcggctctc cagggccgcg tggcccagga cgtccacgtc gcaggcggcc
    26761 cagccccgtt cggccaggag tcgggcgacc aggttcttgc ccgcgcagta gcccccggtg
    26821 aggcccagga tcatcaatgc atctcccccc agcggaccgc ggtctccacg tccacccgca
    26881 gaggcacgga caggcggacc gcgccttcca tctcccgctt catgacctcc gccgtctccg
    26941 ccgcccgggc ttccggaact tccgcgatga gctcgtcgtg cacctgtaaa aggagccggg
    27001 cctcgggcac gctccggtcc agggcccggc ggacccggag catggcgagc ttcacgatgt
    27061 ccgccgcgct tccctggatg ggggtgttca cggccacccg ctcggcggcc tgcttctcgg
    27121 ttttgttccg ggacacgacc gccgggacgg gccgccgtct tcccaggagg gtgcggacgt
    27181 agccgtcctt ctccgccgcg gcgatcgtgg tctccatgaa gcggcggacc ccggcgtagc
    27241 gctcgaagta ggcgtcaatg aacttctgtg cgtccccgcg gccgatcccc agctcgttgg
    27301 aaagccggaa ggcgctcatt ccgtagacca ccccgaagtt gatggtcttg gcgatgcgcc
    27361 gctggtccgg gccgacctcg gactcgggga tcccgaagat gaggctggcg gtccggcggt
    27421 ggatgtccgc cccctccgcg aaggccccgc ggagctcggg atcccccgag aggtgggcga
    27481 agaccaccag ctcgatctgg gagtagtccg cggagaccag gaccctcccg ctctccgcgg
    27541 agaaggcgtg gcggatcctg cggccctcct cctcccggat ggggatgttc tggagattgg
    27601 ggtcccggct ggacaggcgg cccgtggcgg ccccggtctg gacgaagtgg gtgtggatgc
    27661 ggccgtcggg ccccgccagc tccgccaggg aatcgacgta ggtgctcttg agtttggaca
    27721 gggtccggtg gcggaggatc cgctccggca ccgggtcctc ccgggccagc tcctccagga
    27781 ccgatatgtc cgtggagtat ccggtcttgg tcttcttgcc cgtccggagc ttgcgctccg
    27841 tgaagaggac ctcctggagc tgcttggtgc tggcgatgtt gaactcgtgg cccaccaggc
    27901 ggtagatctc cgcctcgatg tcccggaggg ccacgtccag ctcccggccg tagcgcctca
    27961 gctcctcggg atccacccgg atgcccgccc gctccatgtc ccccaggacg ggcaaaagag
    28021 gcatctccac gtcccggaag aggtccgcaa gccccgcccg gaccagctcg ggttcgaagc
    28081 ggagcttcag gcgaagggtc aggtccgcgt cctcggcggc gtatccggag gcctgggcca
    28141 ggggcacctt gtcgaaggtg gaacccttgg gcaccacgtc gtcgtactcg atgccccgat
    28201 agcccaggta ggactccgcc agggcctcca ggttgaaggc cgaccgctcc gcgtccagga
    28261 gccaggcggc caccatggtg tcccaggggc ggcagcgcgc cgccacgccc cagttttcca
    28321 ggacatgacg gtcgaacttg aggttctgtc ccgccatgac caggtcctcc cgggccagga
    28381 gggctcccag cttggccttc aggacggcct ccgggatctt ggttccctcg ggacagcgga
    28441 tggggacgta acaggccttc cggggctcca cggccaggga gaaacccacc ggaagggcgg
    28501 acaggtcgtc cagggcgtcg gtctcgcagt cgaaggcgaa ggtccccgcc cgggccgccg
    28561 cgtccagcca ccggtccagg gaagcctcgt ccgtcacggc ctcatactct ccggggccgg
    28621 aaagccccgc ggggacggcg gggtccacgg cgggcgggaa gggggaggcc gcggccgcgg
    28681 cctccggggc ctccggtccg tggacgcccg gggccgcgac cggtaccccc ggagcggcgg
    28741 cctcgaacag gtccgtggcg gcggtcacgc ccccggcgac gcccgcctta cccgccaggg
    28801 agcgcatgcc ctcccgcagg aagatggggc cggccgcggc ccggttgagg gtccccacct
    28861 cgagttcgga ggggcccgcc acgggcaagg gggcgtccga ggccagggtg atgagggtcc
    28921 gggacaggta ggcgctttcc cggcccgcct ccagcttggc ccgaatcccg tcgggcttca
    28981 cggaggccag gcgttcgtag agcccgtcca gggtgccgta ctcggtcagg agcttccggg
    29041 ccgtcttgtc gccgatcccg gggaccccgg gcacgttgtc cgaggagtcc ccggtcaggg
    29101 acaggtagtc caggatgcgc gcgggaggga cgccccactc ggcctcgacc tcctcgggcc
    29161 ccaagcttcg gcaggccgtg tccttctcgg gccgcagggc ccgcacggag cccccgacca
    29221 gctggaggag gtccttgtcc ccggacacga tccagcattc ccgtccctcc gcgcggcagc
    29281 gctccgccag ggcggcgatc aggtcgtccg cctcgtagcg gtcggccttg agcacgggaa
    29341 tccccagggc ccggaggatc tcctcgacca ggggtacttg ggcgtgcagg tcctcggggg
    29401 tcttctgacg ggtcgccttg taggccgtgt acatctcgtg gcggaaggtg ggcacggcgc
    29461 tgtcgaagac cgccgcgaag gccccggggt tgcgctcctc gaagagggcg aagaggaagc
    29521 ggaagaatcc gtagacggcg gagacgttgg cccccttggg gttcttcagg ggccggttga
    29581 tgaaggcgta gtaggagcgg tagatgatcc cgtagctgtc caggaggtag agggcgggtt
    29641 ttcggctttc catggtcaga gtgtagccgg gaggcgtccg ggtggcaatg acggtggacg
    29701 gtggtcagtg gacggtatct atgaccaagg ccgctcttca gaatccgtta tccgaccgcc
    29761 gaccactgtt cactgtccac ggatcaactt cgccagctcc cgcacgtcgc agggcttctg
    29821 gaggattccg gccagcccca gttccgagtc cagggccagg agctcctccc ggtccgcgta
    29881 acccgtcagg agcaccagcc gggtccgggg gaagcgggcc cgagccgccc gaaggacctc
    29941 gtcccccttc atcccgggca tgcgccagtc gctcaccacg gcggccggct cgccgccgcc
    30001 cgcccggatt gtctccaggg ccgccagggc agactccccg gagagggcgg tctcgatccg
    30061 gtatccggct cccaggaccg actccaggcg gtgacggagg gccaggagga ggacggcctc
    30121 gtcgtccacg cagagcacca gccgatcagg catcggtgtc cgtcccgtag gatctctcct
    30181 cggatccccc ggcggagacg atgccggcgg agggcaggct cacggtgaag accgtccggc
    30241 ccggacggga gtcgaaggcc aggcggcccc ggtgggtctg gatgatctcg gaagccgtcg
    30301 ccaggccgag gccgaagccc tcccccgcgg gcttggtggt gaagaagggc tcgaagatcc
    30361 ggcccgccag ttcctccgga atgcccggac cggaatcctc cacggagacc cggatcccct
    30421 cggggatcgc ctcgccgcgg acggccaggg tcccggtctc gcccagggcg tccagggcgt
    30481 tgtcgatcag ggccatccag acctgctgga gcttgtccgc gtagcagcgg tagggcggaa
    30541 ggccggagta gtcccgtagg acgcggattc cggtgcccag gcggttcttg gacagttcca
    30601 ggacggcgtc gatctgcctc gtcaggtccg ccacggtcgg gactccccga tgatcccggt
    30661 gggtgtagac gcgcagggcc tggacgaccg tggtgatccg gtccagggaa tccgccaggg
    30721 cgacccggta ggcgtcccgc gcacgggctt cccgggcggc ccggatgacg gtctccgggg
    30781 cgtcccggac cagggcgagg gtcccctcgt cgggatcggg gatgcccagg tcggacaggc
    30841 gtgccgccag gcggccgtcc gcgtccagcc ccgcggtttc caaccgggca gccaggatat
    30901 cccggaggga gccgctgtcc gcccggacgg cggcccggac gccccgggcc gcctcaagga
    30961 tatggttcac ctgcgaggac tccggcccgg aaagaaccgg gaggaccggc tcctccgcca
    31021 ggccctccgc cgcgagccgc agggcttgga ggggggaatt gagctcgtgg gccaagccgg
    31081 ccgccaggcc gcccagggcg gccagcttct cgctccgtgc gacctgggcc gtgcgctcct
    31141 ccaccttccg ctccaggtcc gcgttgaccc ggcgcaggcg cgaggtcagg gcgcggatga
    31201 cgaaggccgc caccagggag gcggccagca gggtggctac gacggccggg acccaggccg
    31261 ggatcaccgc ggtctcggtc cgctcgtggc ccagccattt ccgttggagc tcgtagtagt
    31321 acgagtccgg atccgcgacg atctcggcgt atcggtccac gacggcgtcg aactcggccc
    31381 ttcggccgct gttccgggac agcaccgggt acgccttgaa gggggagaac accacggtgg
    31441 tgggctggac ccggcgttcg gcggatacga accagttgct ctgcaccccc gcgaaggcct
    31501 ccccccccga cacggcccgg acgagctggt cgaagtccgg gtattccgtg atctcgcagg
    31561 ggatggccag ggaatccacg tacgtccgga aggcggcgcc gttccggtcg tccgtgacga
    31621 ccccgatcct cttgtgctgg aggtccagga cggactggag gaccgtcccg tgggccacga
    31681 agagctggct ccacccggcc gatacgccct ggtccgggaa gacgaaaaga gcctcccgtt
    31741 cggggttctt gaggagggtc gtgaagaaat cgatttcccc ggagaccagt cgggggtaga
    31801 gctcggagaa cgaggcgacc cgaaactcat agtctattcc cagctcgtcc atgatccggg
    31861 agtagagttc tatgaagaag ccggtgggct tgccggccgc gtcgacgtac gtggtcggag
    31921 gggcgttggg tacgagtccc cagatcacgg gtcccttttg ggcaggcagg gtagacgggg
    31981 cgaaggaggt gagaagcgct gagatacaga gcaccctgag cggagcaggt ttcatccggt
    32041 actccccgtc gacgctccaa ctataggcgg gatccgttcc ggccgcaagg ccgcgtcctg
    32101 ggcttgtcgg cccgggctaa cgtcccggga ctaccgtccc ggcacctcga tagtctcggg
    32161 gtcttcggcg tggcggtgca ggaaccagtc ccgctcgtcc atggcggagt ccgtgatctt
    32221 gtcgatgggc accttcaggt agcggccgac agccgacacc gccacgtccc cgttttccag
    32281 gacgatctcc ccggaaccct cgaagaagcg ggaaccgtcc ttggtgaccc ggccgacgat
    32341 ccaaagatcc tggtccaggg ggatgggctt gcggtacttg gtgttcagtt ccacggtgac
    32401 cccccagacc tggccgtcct tgccgatggc gatggcccgg ccgatggtct cgtccaggat
    32461 ggccgcggcg atcccgccgt gcatccggcc gggatagctc tggtggagtt ccgtcggctt
    32521 gatcttcgcg accagctccc cggactcggt ctcgtagaag gaggccttca ggctgtgttc
    32581 gttggcaagc ccgcagacga aacacatgcg ggaattgttc tgtttcttgg tgacggggtg
    32641 cagcatagga acgattttct catgatattg acaggatgaa caagatgaag aaaggatgaa
    32701 caggatactt gaaaaagatt aattttatct ttttctataa taacggatta tcctgtctat
    32761 ccgtttccag cctgttcatc ctgtctaaaa tcgccggaaa ccgttatcac ctagcccttc
    32821 aaatcgacca aacccgcctc tccggcgtcg gcgtagacgc tccgcttctt agcgtagggg
    32881 ttgttgacgc tcatgatctc gtgccgcagc atttccatcc gctgcttcag gagcttccgg
    32941 ttctccgtat tccgttccac cacctcggtc ttcaggtcct ccagggtggc cttcaaggcc
    33001 ggaatgtcgt cctccgccgc ggaggggtag gccgcccggt agagatcctc cagggggtcg
    33061 atcaccttct ggaaggcgaa gatctccccg acgatcccct gttccatctc gacgtgggcc
    33121 accagggagt ccacgtcccc ggactcgatg tcggccttct ggtgatccag gacctcgagg
    33181 taatgatgga acttgtcccg ttgcttctgg aggagttccc ggaaccggcg gagcaggctc
    33241 ttgcgctcct ccagggcggc ttccgtcagg gccacgcctt ccgtccctta accggcgata
    33301 tttacgcccg cccgggcctc gctgccgctg cccgcggtct gggccgccgc ggtcgcccag
    33361 gcggtcctga gctcgtccaa catgccgcgg atggcgcgga gcttggagcc gtccttgtcg
    33421 atgttggcgg ccaggagttc ccggttgaag tagaggtaga gggcgaagag gttctgggcg
    33481 atctcccccc cggactcgaa gtccagggcg gcggtgagct cggttatgat gtcctggacc
    33541 ttggaaagag ccttgtgggc tccttcgatg tactgggggt gtttcttgac gtccttggcg
    33601 aagatctcga gggctatgtc gcattgcttg accgcctcgt cgtagagcat gacgatgagc
    33661 tggccggggc tggcggtctt cacgcgggtc tcccgatacg ccgtcaacgg attctgggtc
    33721 atctggtctc tccctcccat acggtctctc tacgattctc ggcggatctc gggggttctt
    33781 gactgcatag tgcgggggat ccggtcattc gggaattttc cccgtggagg tccaggtttc
    33841 gatccgggcg taggccgcgt tcaggcgggc ggagaactcg gtggccttct tctgggcctc
    33901 cggggaagct ccgtgacggt ccgggtggtg ctgcttcaag aggcgtttat aggccgcctt
    33961 gacctccgcc ataggggcgc cgaaggcgag gcccaggatc ttgtagtccc cggaaaggcg
    34021 ggcggaggga tcggcacccg gagatcgtcc ggcggccccg gaggagtacc ggttccgggc
    34081 cctgcgctcc tcctcctcgg cccgtcgccg ccgctccgcc tccaggcgtt cctgggcctg
    34141 gcggtcgtcc ttcaggtagg cgtccagttc gtccagggcg tcggagtagt ccgagtcccc
    34201 cgaggagggg cgcacccggc cttcggcggg acgatccccc gcgaaggggt cccggtcctg
    34261 gttccagagg gatttgagca gggtttccaa gcggtcgaat atcggatcca cggtcgtcct
    34321 cgtaggaata ctgtactcca gggagaggat tgaggcaatg gagggagaga aaagagggta
    34381 tatataagga gttcaccaca gaggcacaga gggcacagag aagaggggtc atagaaccat
    34441 aattccaaca tgatgaacaa gataaagata agatgaacaa gatggataat gagggacaat
    34501 gcaatccctt tcctcccatc ttgtcaatct tgatccatct tgtagatctt gttggaccta
    34561 tggtttaaag cccagcatca taagcctccg tgcgctccgt gctctccgtg gtgattccag
    34621 gagatccctc ttcccgggcg accgacggtt tccccgacgc cgcggccccc gacacggcaa
    34681 gccccgcagt caggagggcg atccccgcaa gggccgcggg gctcggggtt tcccggaggt
    34741 agaggatgcc caggagggcc gccaccaggg gttccgagag ccccagggtg tagacccagc
    34801 ccatgggaag gatacccagg gccctgccga agaggaagta gggcagggcc gtggtcagca
    34861 agcccaggtg ggccgccgtc agggcgcccc ggggggagag gacccagccc aggccggaac
    34921 cggaggcaag gtacacgggc acgagcagga ggctcccggc caggagcccc agggtcacgg
    34981 ccccgaggga gggcagggcg gccgaggact tcctgatgcc caagcccagg agggcgtagg
    35041 aagcccccgc ccccagggcc agggcgatcc cggcggctgg ggcgggggcg gccgattttc
    35101 cgagggagac cagggcgcag cccgcggcgg ccagggccgt ggagacccac cacgcggggg
    35161 atagccgctc acggaatacc gccgcgccta gggctcccgc gaagaccggg cccgaggcaa
    35221 tggccaccac ggttcccagg gctacccccg cggcgatcac cccccggaag aagaggacct
    35281 ggaaccccgc cagccccgcc ccggccagga gcacccaggg agaggctccc cgaagcagta
    35341 cccggaagcc gccggaacgg gccgccgcga agggcaggag ggccaggccc cccagggcta
    35401 ggcgcaggag gccgacggac gaggggtctg cccccccggg agccagggcc tgggccgtcc
    35461 cggtggtccc gtagagcatg cccgcggcca gcaccgtggc cgccgtaccc catcgccgtc
    35521 cccgcagtcc catagcgcct cctgaagcgt ccccgttgtt gccggaagtc ccgcatcttg
    35581 gtacactccc tgccatggca gcccggaacg attacgacga atccaagatc aagaccctgt
    35641 cctccctgga gcatatccgc ctgcggacgg ggatgtacat aggaaggctg ggcaccggca
    35701 cgaaccccga cgacggcatc tacatcctcc tcaaggaggt ggtggacaac gccgtggacg
    35761 agttcatcat gggggcgggc cgtcggatcg aggtccggat cgagggcggc cgggtcgcgg
    35821 tgcgggactt cggccggggc atccccctgg gcaaggtggt ggaatgcgtc tcggtcatca
    35881 acaccggagc caagtacaac gacgaggtct tccagttttc cgtgggcttg aacggggtgg
    35941 gcaccaaggc ggtcaacgcc ctgtcctccc ggttccgggt cgtgtcccac cggtcccgga
    36001 agtgcctgga ggcggtgttc aagcgggggc gcctggtatc ccaggcggaa tcctccaccc
    36061 gggaacccga cggtaccctg gtggagttcg agccggaccc ggagatcttc ggggatttcg
    36121 ccttcaacat ggagttcgtg gagaagcgct tcaagaacta cgcctacctt aacacgggcc
    36181 tgaccctctc cctgaacggc aacgatttct cctccgagaa cggcctcctg gacctcctga
    36241 aggacgaggt gggggaggac accctctacg aggtcggctg gtggcggggg gacaagatcg
    36301 agttcgcctt cacccacacg ggcaactacg gggaggagaa cttctccttc gtgaacggcc
    36361 agtacacctc cgacgggggc acccacctct cgtacttccg ggaagggttc ctcaaggcgg
    36421 tgaacgagta cttccaggcc tcctaccggg gggaggacgt ccgggagggg atcgcggcgg
    36481 ccatcgccgt gaagctccag aatcccctgt tcgagagcca gaccaagaac aagctgggga
    36541 acgcggagat ccgcccctgg atcctccacg aggtcaagcg gggggtggac gagttccttc
    36601 gccggaaccc ccaagccgcc aaacgcatcc aggacaagat cgtctccaac gagaagctcc
    36661 gcacggaatt gaacatcgtc aagaaggagg ccaaggaggc ggccaagaag atcgagctca
    36721 agatccccaa cctcaaggac tgcaagtgcc acctcgggga cggggacagg ggcgaggtca
    36781 gcatgatctt cctgaccgag ggacagagcg ccgcggggtc cctggtgagc gcccgggacg
    36841 tctacaccca ggcggtcttc tccatgcggg gcaagcccga gaacatgtac gccaagaagc
    36901 gcacggccat ttacaagaac gaggaacttt acaacatgat gatggccctg ggcatcgaga
    36961 acgacatcga gggcctgcgc tacgcccgga tcatcatcgc caccgacgcg gactacgacg
    37021 gataccacat ccggaacctc ctcctgacct ttttcctgac ctacttcgag gagctggtcg
    37081 tccagggtcg ggtccacatc ttcgagaccc ccctctttcg ggtgcggaac aagaaggaga
    37141 cccggtactg ctacagcccc aaggagcggg acgaggccgt gaaagtcctg ggcgccaccg
    37201 cggaggtcac ccgcttcaag ggcctcgggg agatcagtcc caaggagttc ggccagttca
    37261 tcggcccgga catccgcctg gtgcccgtgg acgtacagac cctgaaggcg gtgcccgggg
    37321 tcctgggctt ctacatgggc aagaacaccc cggaacgccg ggactacatc atgaagaacc
    37381 tggtgaacga cctgtaagca aggaaatccg atggcctacg taaagaagct cttcgacgac
    37441 aacttcctct tctacgcctc ctacgtcatc aaggaccggg ccatcccgga cctggacgac
    37501 ggcctgaagc ccgtgcagag gcggatcctc cacaccctct tcgaggcgga cgacgggaag
    37561 ttccacaaag tggccaacat cgtgggctcc tgcatgaagt accatcccca cggcgatgcc
    37621 tccatctact ccgccctggt ggtcctggcc aacaaggagc tcttcatcga ccggcagggg
    37681 aacttcggga acctctacac cggcgacgag gcctccgcgg cccggtacat cgaatgccgg
    37741 gccacccccc tggccaagga gatcttccac aaccccaaga tcacggagta cgtggattcc
    37801 tacgacggcc gcaaccggga gcccgtggtc ttcccggcca agatccccgt ggccctggtc
    37861 ctgggggcgg acgggatcgc cgtgggcatg agcacgaaga tcctgcccca caaccccgtg
    37921 gaggtcctcc aggcggagat cgactgcctc cggggccggc ccttccggct gtacccggac
    37981 ttccccaccg ggggcctcgc ggacgtgagc gagtacgagg acggggccgg ccgcgtccgg
    38041 gtccgggccc ggctggacac ctcggacccc aagcgcatcg tcatccggga gctccccttc
    38101 ggctcgacca cggagagcct gatcgccagc gtggaggcgg cggccaagag cggccggctc
    38161 aagatcgcct ccatcagcga cttcaccacg gagaccgtgg agatcgagat caagctggcc
    38221 cggggggtct actcccaaga gaccgtggac gccctgtacg ccttcacgga gtgcgagcag
    38281 gccgtctcct gcaacctcct tctgatcaag gacggccacc ccaccctcat gaccgtgacg
    38341 gaggtcatcc gccaccacgc ccggcgcctg gtcgggatcc tcaaggccga gctcgagctg
    38401 gaggcccggg aactggccga cgagatccac gcccgcaccc tggagcggat cttcatcgag
    38461 gagagggtct acaaggccat cgagaggatg cggacccagg aggcggtccg gaaggccgtg
    38521 atcgacggcc tcgcgccctt cgccaaggag atccgccggg aggtcacgga ggaggacgtg
    38581 gagcgcctgc tcaagatccc gatccggcgc atctccctct acgacatcga gcgggccaag
    38641 gcggagatgg cccgcctgct ggagcgcctc aaggaggtga agtaccacct ggcccacctc
    38701 acggactacg cggtgggctt cctcacgggg gtgatgaaga ggctcgagcc cgaatggaaa
    38761 cgcaagaccg aggtggcggg gttccgcaag gtggacgtta aggaggcggc gcgccgggac
    38821 gtggccctgc gctacgacgg gcagaccggc tacctcggga cctccgtgtc cacgggggac
    38881 tccctgttcg acgtctcccc ctacgacaag gtcctggcgg tccgacggaa cgggatctac
    38941 acggtctttc cggttccgga gcgggctttc gtggaccagg gcctgctcta ctgcggcctg
    39001 gcggaaaagg agctcctcgc ggacgtcgtg ttcaccctgg tgtaccggga cgccgggacc
    39061 ggccaccccc acatcaagcg ctgccaaatc gacgcctgga tcctgaacaa ggactaccag
    39121 ctggtcccgg agggggacca gatcctctgc ttcaccaccc tgccccaagg cacgctcacg
    39181 gtgaaatacg cccccaagcc caagacccgg gtcctccagg aaaagttcaa gatcgaggac
    39241 ttcggcgtaa agggcctcaa ggcccgggga gtccgtctgg cccatcggga agccctggcc
    39301 gcggagt
//
LOCUS       DAOTEI010000010        38261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_714_length_38261_cov_6.516097, whole genome shotgun sequence.
ACCESSION   DAOTEI010000010 DAOTEI010000000
VERSION     DAOTEI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 38261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38261
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_714_length_38261_cov_6.516097"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="P5313_01720"
     CDS             <1..848
                     /locus_tag="P5313_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HRY79112.1"
                     /translation="KAAADRGLGRVAVFTKPREAAVFEGLGFRRLAAAERPATGLGAS
                     LLEWGSPGIGDWKEDLARRAEGRPRPSGVVVVNCNPFTRGHQALLEWAASESSWVHVL
                     VVEEDRSLFPFPVRLDLVRKGTTHIRNLDVHPGGPYVISSATFPTYFTRPRPGSPDDA
                     AAELHAALDLEVFRTHVAPVLGAVERFVGTEPYCPTTSTYNRLMREILPAPRPDSPAL
                     EIRELPRVERDGAAVSASRVRDLIRAGRFEEIRTLVPETTWDWLRSPGAAPVLERIRA
                     SDTRH"
     gene            950..1222
                     /gene="citD"
                     /locus_tag="P5313_01725"
     CDS             950..1222
                     /gene="citD"
                     /locus_tag="P5313_01725"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HRY79113.1"
                     /translation="MKAKKEAMAGSLESSDVLVTIRPREGEGVECTIESIVLKQYGRR
                     IRSVTEEVAAAAGLSGAAVRVQDRGALECTLRARLETAIERSTAGT"
     gene            1219..2586
                     /locus_tag="P5313_01730"
     CDS             1219..2586
                     /locus_tag="P5313_01730"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HRY79114.1"
                     /translation="MSSPALDAREERWARRLSLAGSLPAGGSLLTLTLRLPAALRLGG
                     DFDGAARALFEALEAEARTSGSPVLSSEFKISADGPEGWLALGTGARAAKTLALGVEE
                     DHPWGTLADIDATDARGQPLGREDLGLPPRTCLVCGGPAAVCAAGAAHGLPELRAAAE
                     TVLARPACTPGRIGRLALQALLEEAAVDPKPGLVTPRSRGSHADMDYFTFLAGAAALA
                     PWFAEAADLGAGLAGFASTEDPGAQDLLDRLRAAGRRAEAAMFEATGGVNTHKGLVFS
                     LGALCAAAGCLAARGFVPTAESCCDTAGALVRAAATADFTAASAKPEAELTAGERLYR
                     SSGVLGVRGEAARGFPSALRIGLPRLRSGLAAGLSRNDALAEVLLALCAEVEDTCVLA
                     RGGPEGLEYLRAEAARALGLGGLSTSAGRAAVRNLDEGLIARHLSPGGCADLLAVTDF
                     LERLG"
     gene            complement(2649..3680)
                     /locus_tag="P5313_01735"
     CDS             complement(2649..3680)
                     /locus_tag="P5313_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY79115.1"
                     /translation="MKTLKVLPIVLASTLWILSCGSKPADSSGTGTADPSVPGQGFLS
                     AGQPVTGVQPQGMQAADTSWIKVKHLDLAYASKSPTQKLDLYLPNEGSGPFPLIIEFH
                     PGGFMVGGKSMGIGPMLEGLKRGYAVASVGYRLSGEAPFPAAVNDAKAAVKFLRASAV
                     KYNLDPRRFAAWGSSAGGNLAALVAVSGDDPSLVDSSLGNAGVSDSVRAAVDWYGPLD
                     FSAMDEQFKALGTSGSMGPTSSPTSPESKYLGKTVGTPEARPLAEKANPGTYISADDP
                     PMFIQHGTADRNVPITQSRDFAARLSAEIGADKVIFQALEGAGHGGTAFEAPENVARI
                     LDFLDRHLK"
     gene            complement(3741..5186)
                     /locus_tag="P5313_01740"
     CDS             complement(3741..5186)
                     /locus_tag="P5313_01740"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY79116.1"
                     /translation="MSLLALLPAASSVAVGSVESFTRRLDARISGLMRRYEVPGLGIA
                     LIRDGGIVWQKSYGIADSGRPLNEDSVFRAESISKSVTAWGVLTLVDRGLIDLDAPVS
                     RYLGDFRLPDSPYEEDTITVRALLSGNAGMVLGPVGPSVEYAPGAPKPTLREALAGTV
                     RMNREPGTGFEYSNVGFNLLELVVENVSGRPFAEYMAEEVLQPLGMTDSSYEWNATIR
                     ERSASGHDLKGRRVGAYVYSEKGSGGLFTTVRDIAAFVRAGMRVPGGEGAGVLTAGSL
                     GSLHSDSAEIRGLFGMAADAYGFGHFTERLSDGRRAVWHGGQGHGWMTHFHAVPESGD
                     GIVILTNTQRSWPVISVLLADWSRWCGFKPVKFSRIRFGVAGMWVLVGVLALATLYSG
                     GRLAAGIARGRLRFAPLAPEGRAGRILRAAAGTAGIAAIGWAASRPYLMVTSLFPGPA
                     ESAGGAVLALSSVLLFSALFSAPAAVRSARF"
     gene            complement(5252..5452)
                     /locus_tag="P5313_01745"
     CDS             complement(5252..5452)
                     /locus_tag="P5313_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79117.1"
                     /translation="MTLILRISNLLSFLRKAEAARTTRVERAAGRAAKNRMSGLRSDA
                     EEFGSRAAADAWQYACNQRAFR"
     gene            complement(6093..6512)
                     /locus_tag="P5313_01750"
     CDS             complement(6093..6512)
                     /locus_tag="P5313_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79118.1"
                     /translation="MARRAVCWILVLVAAAAGAQTITFGLGDAGLEASLNEIGASARV
                     DLPGFTAEVSLQWGIPKTQVQVVLSQGFQPAEVYLVAALSSLSGKPVATVVDVYRKNK
                     AKGWGFVAKELGIKPGSKEFKALKDKSTASASKAKKK"
     gene            complement(6625..7821)
                     /locus_tag="P5313_01755"
     CDS             complement(6625..7821)
                     /locus_tag="P5313_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79119.1"
                     /translation="MRDSSRTVLLLTLAHFSHHVLTALLTPLLPFIRDDLGLSYAQVG
                     VVLAAFNLSYGFGQIPAGRLADRIGARVVLTAGIAGIGGAGLLLGLSSGYSLLLASLV
                     LMGILGGGYHPAASPLITSAVDPKRRGRALGLHAVGGSISYLAAPLLGVAAAAVLGWR
                     GTFLAFSVPVVLLGILIHRACLRASLGGPAVAGPADGGRRDRLTPGTILELSAFILMV
                     TGMGAALASASSFLTLYIVDVLGATERTAAALLALYFSAGVWAAPLGGRLADRFGRKR
                     VAAAAAFAAVAGLLALPRVPYGFGFFALLAALGAVPYVRMPAAEAYLVGRVPENLRST
                     VLGLYFFAGMEGSGVLTPLLGRWIDLHGFSAGFTALGFLLACVVAVCSAALAAASRNE
                     ARGTAV"
     gene            8014..9144
                     /locus_tag="P5313_01760"
     CDS             8014..9144
                     /locus_tag="P5313_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79120.1"
                     /translation="MNDGWLASLPLLLPFLRGELGLDYAGIGILTSLLGVAGIFLALP
                     AAGLARRFGGFRVLAAAAVLYAASFLVVAAARSYGALALAFGLASLGFGAFHPIGFSL
                     TASAGPSASLGKRMGGFTAVGDIGRIGVAALVTALMAWLSWRRVAAIYAAVPILVLAA
                     CLAGGLFRDANDAGTHGLPETGKNRRFRLSVDRRLAPILASGFIDTLAGSSLFVFLPF
                     LFTLRGASPGLLGSLSGAFFLGNMLGKVALGGTVDRFGGRRVFFVSEFVMAALLVALG
                     RSESLAVLVPLSVLLGAVTKGTVPVINTLLAEAAGEGGGVEEAFALGSTLNGVAGVIS
                     PLLFGLVSERFGVDKVFPVSAAFAVLAVLPLLITRKRGAARS"
     gene            complement(9172..9669)
                     /locus_tag="P5313_01765"
     CDS             complement(9172..9669)
                     /locus_tag="P5313_01765"
                     /inference="COORDINATES: protein motif:HMM:NF023540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HRY79121.1"
                     /translation="MRIVYDVPGHVPGLRAVSVVRGGRSSEPVLVVRLAEDGPSGNGE
                     SAGRPAEAGFPAINSERVRARDAAVRAHEAAHLSTLGPYAASAVLYDTVATPDGPAAV
                     GGRIAVDLAEVPGDPEATLRKARVILAAAGAPGDPSAADARVAAEAYRLMAKAVAELS
                     VDERA"
     gene            complement(9749..10348)
                     /locus_tag="P5313_01770"
     CDS             complement(9749..10348)
                     /locus_tag="P5313_01770"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HRY79122.1"
                     /translation="MKTKHVLWIFLAAMAGLVAFAVLFRAEFTALLNRPDAYRHALFV
                     HVAAATMFFANAVVGMLWERRSLASGRKDVILHTYETVAWLDARLSSPLILLSLVAGL
                     MLSVLMGDLWGIGWLASGFVLFLLSGLVWVASDIPTQYRLKRLMAGLDPADSALPEEL
                     IRLLRLRWWISLAGVLPLVVVFALMVYKPDIPAPAAWFR"
     gene            10430..12016
                     /locus_tag="P5313_01775"
     CDS             10430..12016
                     /locus_tag="P5313_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY79123.1"
                     /translation="MIKKDIVVVGGGIAGLTAALSLAHKGKDVLLLEKNERCGGLMNS
                     FVRDGFRFEGGARALVNAGLVKPLMKEFGIDLEVLPNPITLGIEDRTLVIAGEKSLDD
                     YARLLKDLYPGSEGDVDRIIRAVREIIEDMKVLYGVDNPLFAQKKKNILVLLPELLAW
                     TIRFFRTMYHISQMDTPFEEYLDAISSNRSLKDIIGQHFFRKTPVFFALSYFALYNDY
                     IYPKGGVGALIDTLVDTIRARGGEIRCGTEVTRIDAKTRILTDSAGNSFGYSRLIWAG
                     DLKTLYALSGEEGLAPKDRASYARRKEAVLGHRGAESVFSVFAAVDLPPETFAKAASG
                     HVFFTPDRRGLGTIHTAEVRDLLRRWDSVRKDELFAWLRRFCAFNTFEISIPVLRDPD
                     AAPPGRTGIIVSALFDYELTERIRSSGWYEEFKSRMEDEFLKTLSSSLYPGLEEKILF
                     RFSASPLSIRERVGSSEGSIVGWSFEEGIPAVTNMFRMADSVKTPLPGVCAAGKWVYS
                     PAGGPTAIMTGRIAARRCLR"
     gene            12181..13623
                     /locus_tag="P5313_01780"
     CDS             12181..13623
                     /locus_tag="P5313_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY79124.1"
                     /translation="MKAPRRIVSVVLLVILASYMIIAQSIEADYPGVRSDPLVFPPPT
                     GREKGLIESGRTDVSDYHPLSDFGPVADPFLDEIAAKALWHRRQGDPAKAEELYAYVI
                     WYSATNNLALTNGQLNGMLQLRAFSLSHLKRNEAAARVFDFRIAMLEDRSWRERLPAR
                     LVVHDALDDRTRAGLLAFAYRDRALERTITRDFDGAWADAGASAAEFNKIGATRDVLY
                     TYEIFITNATLLGGYDHVPAAYDAFTKAFSGGGKQEADRMLRISGFAAQAYLKLGDIV
                     GAYDTLNLMVKLSDLWGYKDPMVTEFRSLRDQLKPLVEAMPKRSPGSLVDFLRSGDAA
                     AVRRILAEGADPNLPDDTNTTPLMYACRSGGSIEIVRLLLEAGADPNIRGENGITALS
                     LAGGFTSQVDIVRALLAAGADPNARSKAGWTTLMLVARNGTNPEMLRVLLEAGADPNF
                     RTNDGVSALHLARNSGNKEIIRLLLEAGAR"
     gene            complement(13744..14391)
                     /locus_tag="P5313_01785"
     CDS             complement(13744..14391)
                     /locus_tag="P5313_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY79125.1"
                     /translation="MKNETLSERIFTKLKDDILSGVYVPGERLLYEKIAERLGVSMTP
                     LKDAFHKLEQEGLVNNVPRRGCFVTRLDDEDIVEYCQIRWALESLAVDLVCAKSSVPE
                     GDLDGLEKINDQIAKAIRRKRPRECIVHDAQFHLSLVNMSGNRRLIQMLHQFPLSNLL
                     VFMGRGEKSIENGEAILDDHARIIEALKVRDRQRIKEILEKNIFVPYNQIIGERR"
     gene            14616..15995
                     /locus_tag="P5313_01790"
     CDS             14616..15995
                     /locus_tag="P5313_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRY79126.1"
                     /translation="MHKVSKPFAFSKMKDAEQFAREMHAAGLPFPVSSDMSILGEPVR
                     LGAKTIPNRICLQPLEGFDGTKEGAPSDLIFRRYDRYARGGAGLIWYESITVADDGRC
                     NPLQMIIKKSTIKEIRRLVEETDRTAYEAFGRKPYNVLQLTHSGRRSVDANWKSTPLA
                     VVANPYIDHHNSIDGQSGKITIASDEKIEQIIREFIEAAVLSVEAGFDSVDIKVCHEY
                     ILRELLAAFNRPGKFGGSFENRTRALFDIIDGVRARVGDAIDICVRLNAYDCIPYPHG
                     WGMVKEEGVMAPDVSEPIRLCQMLVERDVKLINLSTMMPRYQPYGRGYLAELEEDAEI
                     QPYKGTYELLKATRQIKEAVPEGIFVCTGLTWLEQFGGNVGAGGVRDGWFDIAGFGRQ
                     AFAYPDFARDILIRNVMEREKCCMTCDKCYDLIQKGHTVTGCVMRDQEVYLPLYRKHV
                     QKREVEAVR"
     gene            16040..17026
                     /locus_tag="P5313_01795"
     CDS             16040..17026
                     /locus_tag="P5313_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY79127.1"
                     /translation="MHYRKLNRTDLEVSPICLGTVNYDTALLSKADSKRQLSRFVERG
                     GNFVDTAHVYGMWEPGATSRSEAAIGEWIRETGKRDSLVLATKGAHPEWGRMSVPRVR
                     PRDIEADLEESLRLLGTDHVDLYFLHRDDPAVPVSDIVDCLDRARERGKIRWYGCSNW
                     KLGRIREAAAYAAGKGSPGFVVNQLMLSLADINFHNLPDKTFVLMDRETAEYHAETGL
                     NVMAYMSIAKAYFTRRHQGEKLPESVTSVYGSESNDRIYERARAAVDAGEYTFMDLSL
                     MYLMAETRFPTVPIASFDTPEQLEEGLSCWDKPVPGALLAELGRMKRYVYFG"
     gene            17064..18332
                     /locus_tag="P5313_01800"
     CDS             17064..18332
                     /locus_tag="P5313_01800"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HRY79128.1"
                     /translation="MKESRIREGYELAKEAYGELGVDVDAAVRKADSIPISMHSWQGD
                     DLIGFDGVGELTGGIAATGNYPGRARTADELRADLDKAMSLIPGTKRVNLHSCHAETA
                     VRKVDRDAYAVEHFRSWLDWAGARGIGLDFNPTFFSHPRMDGDFSLASRDAGTRAFWI
                     EHGKRCREIGAEFGKALKSPCIVNFWMPDGYKDTPADTAAPRTRMAESLDRIFERKFD
                     PAHLRDALESKLFGLGLESYTVASHEFSLGYAVTRKILYTLDAGHFHPTETISAKLSA
                     LLPFVGELLLHVSRGVRWDSDHVVSWDDELQNIMNEIVLNGYERQVRIGLDYFDASIN
                     RLACWVIGTRNARKALLAAALTPVAAMRKAEDSGDYTTRLALIEEAKSLPFGPVWDHY
                     CLESSVPVGSDWLDEIRRYEREVLSARGRA"
     gene            18342..18725
                     /locus_tag="P5313_01805"
     CDS             18342..18725
                     /locus_tag="P5313_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010348477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HRY79129.1"
                     /translation="MEPIGSRTWIIPDMYWPEHTSPGRYVSHEAVCVLNPSDADCEVV
                     LDLYFEDRAPIRGLRALCPAARTRHIRMDALKSPDGSPVPRGVPYAAVVTCSVPAVVQ
                     YTRVDTTQSENALMTTLAYPASGRG"
     gene            18744..20264
                     /locus_tag="P5313_01810"
     CDS             18744..20264
                     /locus_tag="P5313_01810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009427843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRY79130.1"
                     /translation="MRYYLGIDNGGTVTKAALYTKDGREAAVESRRTEVLSRAPGFAE
                     RDMDDMRETNFEVIRCLLERSGIPGRDVAGIACCGHGKGLYLWGRDGRPVRPGILSSD
                     NRAWEYPERWNRDGTAPKVFGKTFQRILACQPVSLLAWMRDHEPEKLPEIRWIFECKD
                     YVRFCLTGEAFGELTDYSGANLVNLETRDYDPELLELFGLSGFRDRLPPLRSSTDLCG
                     RITPDAARRTGLAEGTPVFGGMFDIDACAVAVDAADDDTLCMIAGTWSINEYVRSEPV
                     RDGSVMMNSVFCVPDRYLIEESSPTSAVNLEWFLSTLTPGAAASKDAYAAAESWVESV
                     PRSDAYPLFLPFLMAGVPHPNAKACFLGMTYYHTRAHLAKAVYEGIAFSHRYHVDRLL
                     ASRGRPFDRIRLAGGVTKSAGWVRMFADVLGSPIETVDIRETGTLGCAMNAAVSLGDY
                     ADFGAAARGMIQVRPAVEPDPSKTLEYDRRYRLYRAALDALDPVWDGIQGLYDPTP"
     gene            20377..21399
                     /locus_tag="P5313_01815"
     CDS             20377..21399
                     /locus_tag="P5313_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY79131.1"
                     /translation="MGRKWLLFAALLALAGSVFAQGSKKITMVGIDKVVSAKEFGYDA
                     KPKGKYTIAVVVKSAAIPVWESHLIAAEKAGKKLGVKIIGYAPTKADNVEEQKRILED
                     LITTGVDAVILAPANTEAVRGPVLSLIKAGIPVIYDNTMGPSNVDYLTYVGVDNLEVG
                     NVIAKTIGDMMGGKGKLLVMEGVPGQSTSDLRTEGVMKSLAKNYPGIVAERAVTKWQF
                     DEGRKVTEDYITKWGKDLKAIVAVGGNQAEGAVEAVKAAGMNGQVLISGFDVQEPQYT
                     AVANGDEVFTVSQGVYDQAYLSVVAAIRALNGESVPRLITTPITVVTKKNLTAMDERP
                     AALKAR"
     gene            21563..23089
                     /locus_tag="P5313_01820"
     CDS             21563..23089
                     /locus_tag="P5313_01820"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY79132.1"
                     /translation="MGTPEYLLEMRNITKTFPGVRALDGVSFDLRPGEVHVLLGENGA
                     GKSTLMKVLAGAYIPDSGELYIDGRLVDHFNPRHAQSLGVGIIYQEFNLVPYLNVAHN
                     IFIDHMPRTLGGLALDHARMHRDAEALLKSLDMRVDTRALAHDIPVAQQQMVEVAKAL
                     THDLKILIMDEPTASLCDREIEQLFRTIRELTARGIGIVYISHRLQEIPLIGDRITVL
                     RDGRYVGTANAADLTQERMVEMMVGREISNLFNRSYRPRGEPALRVKDLSSRGTGLSN
                     VNLTVHRGEIVGLAGLVGSGRTELARAIFHVDPHETGEIELLGKSLAKEDPKGAIRRG
                     LALLPEDRKRQGLALILSVAENVVMASLSRLFPRGFLRNRREQESVSKYVQELRISTP
                     GIRQFAKNLSGGNQQKVVLAKWLCTEAEVFMFDEPTRGIDVGAKQEIYAFMDDLARQG
                     KAVLMISSELPEIIGLCDRVYVMREGSIVAELDRTEATQETIIAYAMGGVCADEGRAS
                     "
     gene            23086..24036
                     /locus_tag="P5313_01825"
     CDS             23086..24036
                     /locus_tag="P5313_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79133.1"
                     /translation="MSGFSRILKNVPAVLWVELLLVIVFSFFSRNYLTTRNLLILLQQ
                     GAVLLVISAAATFVIISGGLDLSLGAIMTVAGVFTALAVNAGFPIPAVIAVGALVGTA
                     CGAVNGLLISFAGMEPFIATLGMQGLLYGISLAMTNKEGIPVSNERFVFLGDLVNRTV
                     PMAAISCGLIFLLAVFVQNHTLLGRYTFAIGGNEEGSRLSGVETRFWKWAIYAFAGFL
                     TGLGAVVLVARLEVADPIVGVQWEFEAIAAAILGGTSLRIGRGDVRGTIVGVMLITII
                     RSGLNVVRVPSVWQPAIIGTIIILSIVFQVWISTSGKERK"
     gene            24033..25019
                     /locus_tag="P5313_01830"
     CDS             24033..25019
                     /locus_tag="P5313_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79134.1"
                     /translation="MTKTSSGVRIRADAALLAGLGRFGALAILIVVGALASDVFLSGR
                     NISNIVTNASVIIILGVGQTIAIITNGPDLSAGSVLTICAVIATLMVKQLGIFFPIAI
                     LAALALGMFLGFLNGYMIARIGIPSFISTYGLQWAVFGFAYVILKGYVIYDFDTSFRF
                     IGNGYLFGTIPMPIVVMLLVVTGGMFMLRRTVVGRELYAVGANRVAARMSGVDTEKKI
                     IQAFILSGALAALAGIVLVARINAVQADIGKNYVLPVIAAVFMGGTSPTGGRGTLLGT
                     VIGALIITVVQNGMNLLAVDSVWRDAVIGVLIILTVLIDITMQKRLARIQAA"
     gene            25099..25851
                     /locus_tag="P5313_01835"
     CDS             25099..25851
                     /locus_tag="P5313_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY79135.1"
                     /translation="MDLLTDLTFLHWLTIALAAVLVGSARAGLDGGPLIAIPLMAGVF
                     GARLSSSIMLGIMLTADLAALFKYRSLGSFRHLLRTLPYALAGILLGAFVGGRLSEET
                     FRTVVSVLILLSAAMMLAQELKAGGFVLPERWYYAAPLGLLSGFSSMVGNAGGPIMSL
                     FLLSSGLLKNQLIGTMVWFFFIINMMKLPFHIFAWHTLSVPTLLVDLAVAPLAVASVF
                     GSARLVQRIPEKPYRFFRLATVALGGAYLLFT"
     gene            25888..26229
                     /locus_tag="P5313_01840"
     CDS             25888..26229
                     /locus_tag="P5313_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY79136.1"
                     /translation="MTIVRSDAGGTYTPPGHDASVRSRKLFNPSNGCPKLDAHVTTFA
                     PGAGMEEEIHEVSDHVFFMLRGELEVFRGAEAAGTIGPGDAVHIPAGEPHRLRNSGPS
                     DAVFLAVTTLP"
     gene            26226..27104
                     /locus_tag="P5313_01845"
     CDS             26226..27104
                     /locus_tag="P5313_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HRY79137.1"
                     /translation="MKTDDRPPLPPRIGIYEKALPAGLDWPGMLRAAREAGYDYVEMS
                     VDETDARLARLEWSAGERAAFREAVADSGLRVPSMCLSGHRLYPFGSADPAVRERSRD
                     IMVRALDLAADTGIRTIQLAGYDVYYEPSTPESREKYREGLAEAVELAERAQVCVAME
                     IMDTPFMGTVSRWLEFAEELPSAWFQVYPDIGNLSAWAEDVPGELAKAKGRIAAVHLK
                     DTLKPRPGFPGRFREVPFGTGCVDFPGAFRALRSIGYRGSYLIEMWTHQSPDPAAEIR
                     AARQWILGRMREGGVA"
     gene            complement(27685..29238)
                     /locus_tag="P5313_01850"
     CDS             complement(27685..29238)
                     /locus_tag="P5313_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79138.1"
                     /translation="MVLSPEEIRAVPFASAPLPVLLKPLGALKSEAERSGLLVIEDLA
                     RVIDHPVLGNLLSLAVDGMSPGDLAAFGLGAAVGKDSGEATRILLFTFFAVLLQTASR
                     DTESELRSFLERKSPCRSIADALIRGTMPLHYERLLGGKPGLREIVEGAGASDAHSLY
                     IGGLLSLGLPDLGEAVDLLDRFRSAYGRIHPFQWAFQDYLSTLEVEKGGTEGLLGVLR
                     FGFLAGPAMSESFHLLSRVRDTGRAGPFTNFLLGMLHLYREESVPYDAEKAFALVLDS
                     ADQGSALGAEWKAGFLGSRQTPAFPGNAVPRSIRGTEVFSAGEIDLLLAAFGDPGAGE
                     NPAVPAWYDPERAAEDLLGPSPGWSGLCLRARAVRAAAAGTTDEAWGFVRRCAVLGES
                     WAVATVVGAWSGSTLGLFAPKEDGLSGGLVIPSGLSASSLVKAIRGDRRSRPIVVLAA
                     RHPLHRTDAAALAERLEGGGAPRDPELQTLLRTELDKAEAADRNAPGAMTQAEIDELL
                     GNTPRVESQ"
     gene            complement(29279..31051)
                     /locus_tag="P5313_01855"
     CDS             complement(29279..31051)
                     /locus_tag="P5313_01855"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRY79139.1"
                     /translation="MSRTLILHRKIISFLLLLLSAHLPSVAQDAARSGEFLFHRTLSG
                     FETWFAESPRGDLAVAGLAEQGPVVLLERSTGLVLRELSPNAQAFGAAFSADQDLVFV
                     TERYILKVYEVRTGRVRSVHRVEMEGPYLVVAGRVLFLRGRVDGSEGLFRLDPGGPGT
                     GEDGTLTRVPALPPGIVLGGTRAAAADKDPLPSDLRKTFSRVFPPRVVGRLSAAVLAE
                     GAVVLLDLRTGRPGRILDLDGSEPKLFETDYFGRIGAAYAPRGSDHYLHLWHLDSATA
                     PAVQRPDNRYQVPLHPVRLAISNSGTEAWISGFRGGRDIGGGRIIETVIAAGFLDLKT
                     GSWKESAKDEAPSWAVEKTSLGVEELVQPGRLRYRMLDHGVVEARDPKTGDPLVHLSV
                     DGRGEWAAFAPDGRWDASPGALGSLLYRSDGLRTWAPDPARDGGFTPGLVQALAEAAE
                     SIHLSVNVDRGEGGVYRIGEEITILVSVDRPVWLRLYYIQEDGTAKLIWPSDHVRKQW
                     IPAGTDQRFPPVDDWYSFKLTPPLGRESILAFASTRPFEPGHPGLPDTGKQDQGSSVP
                     RYITEPESWPPASASARTTFTVTE"
     gene            complement(31237..33753)
                     /locus_tag="P5313_01860"
     CDS             complement(31237..33753)
                     /locus_tag="P5313_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRY79140.1"
                     /translation="MLIKTILILVMVFLFDIEAFPEEQSYPITVPPDSSVVDVYGAKK
                     NYVAVIKSTAGYSIFSNFFPRQGPYKWIGSDNLRLSPDGEKLAFSYDDFSGASFINVS
                     GKVYGPLTGYIWNGTFSPSGDEFAFVRTTDSKNYLHVGSSVFGPYLEIGYVSYLQGVG
                     FIVQAKNEKGWYSFEGSRMWGPFSEMGEYWVLSDGKTLVYSAKRIDDTKSPTPVWNLY
                     TSSGRSIAGPYLEIEQLTISPDGKNYAFMFKSDDGWRIRSQSVTVGPFHNIFGRFAIA
                     NNGTLAYIVEGKNGRRTLYRNDTATGIWGDLFDPSISSNGNLIAVYVFRDGRKCDLIT
                     QFGKEEGCLWPYENVLDAHISVDGTHLLMAVKNKDKIQIIVNNSLLMDVSDSLKGTGF
                     RPEDIDLYPSPRWGRLAFIVKDPSFVRSNGDDGYCLLFDGAMWVGQFERIMNLNFAPD
                     SSSLSYSVSERGVMGARENHLSRIDSTHKRWYTGRSYSSGSAYFDGTGSIIVRYVDSI
                     APSIGVATETSGSLSLKLASPGTLIVGNVTMTVSAGTVPVNDLPAGPHCVTVRYEDGK
                     VESYVGMVSAGEATALSFTYIPMPKATRSLDKSKSLRGEIFVQGGTFRMGSESGRPNE
                     KPVHSVTVSSFWMMKTEVTQKDFIALMRMNPSSFEGDDLPVEKISWYDAIDYANRLSQ
                     KDGLTPAYTISEDKVSWNKGADGWRLPTEAEWEFAARGGVSSRGYTYAGGNEVDAVAR
                     YNKKGGHGTKPVGTYGPNELGLYDMSGNVSEWCWDWGWSYSDAAQIDPVGAASGDVRV
                     TRGGGWKQDAIGVRVAFRGQRAPFGSGEDLGFRLVRPQIR"
     gene            33922..34887
                     /locus_tag="P5313_01865"
     CDS             33922..34887
                     /locus_tag="P5313_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRY79141.1"
                     /translation="MDSDWHNQAETLFESATRIAAQIRLGGTTGLSMLPNHAYPPIFN
                     LGLDMARKGAARADIVEVIEILMASSGGSFAHDNINRFSLAALLLVLDGEGPDALREE
                     REAAILGRSRLPGRDRRPPVFEVSPDGIESRLNWIDTRVSSRQDLLIKGNVSDELSVD
                     SSGSIRVVGQVNAAHIEAEGSIKVEGCVFGKGRAVIRCGGDFHAISLERARLESGGDV
                     HLGATVEPIELTCTGDLICEGGPGVILGGSVRAGGSVHAGMIGGPLCTCTSVSAGAGI
                     TVTGTIHERTEISVGQSRMSLRQALTCVRIREEGFLLRLEAADAF"
     gene            34877..35329
                     /locus_tag="P5313_01870"
     CDS             34877..35329
                     /locus_tag="P5313_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79142.1"
                     /translation="MLFDVSEIRAASCSREERAVLAGLADRMAEMAGTVEKEGLFALG
                     RYAEEHTGFLAEAVGFALEGSGGAKTALREAALSSGKRGRALLERLMISQGLAGITSG
                     EGADLVRLKVRAYTGTEFFFREDQEEAIQSFMNLGFDRSTAEQLSGIN"
     gene            35721..36728
                     /locus_tag="P5313_01875"
     CDS             35721..36728
                     /locus_tag="P5313_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HRY79143.1"
                     /translation="MENIMRKSWLAIGLIVVVTLVLEACGTTLQDPVKASSKSITAFS
                     FVGGEAAIIDQEQRVIHVNLDSRINMTGLVAIFETTGVGVSVDGVLQLSGSTPNDFSY
                     PLRYLVIAEDGTMADYLVIVDPPLIDECCIGVLVDSFMGDIDWSAARNDMVDYAFIRA
                     RQGEFPDAKFSANWAGARAAGILRSAYIYVDFNKDVEESTNLFLADFTGDYKSFDLPP
                     LILCEAEGATSEAYRLNWLQLCVGLIEAEIGVKPIIATGTLFWSGSMGNSDAFTEHPL
                     MIRHYTTAIVPTFPSAWHNWAFWNYTGTGTVNGITGDVTSMLRLGWTKKRSSLFPHSL
                     P"
     gene            complement(37504..37851)
                     /locus_tag="P5313_01880"
     CDS             complement(37504..37851)
                     /locus_tag="P5313_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009113182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79144.1"
                     /translation="MSSIPRNSPFNVAVSGIDTLPHLPVIVNLATKYCWWKTPEESLA
                     YPQRVIAAIMDKGTFSDIQLLLSLIGEEGLRDALSQAEPGQFRPRSWAYWQYRLNGLY
                     EHSLPALPSRVIP"
BASE COUNT         7172 a        12140 c        12098 g         6851 t
ORIGIN      
        1 tgaaggccgc cgcggaccgg ggcctgggca gggttgcggt cttcaccaag ccccgggagg
       61 ccgcggtctt cgagggactg ggattccggc gcctggccgc cgcggagcgg cccgccacgg
      121 gcctcggggc gtccctgctg gagtggggca gcccggggat cggggactgg aaggaggacc
      181 tggcccgccg cgccgagggc cgcccccggc cctcgggggt cgtggtggtg aactgcaacc
      241 ccttcacccg ggggcaccag gccctcctgg aatgggccgc ctccgaatcc tcctgggtcc
      301 acgtcctggt tgtggaggag gaccgctcgc tcttcccctt cccggtccgc ctggacctgg
      361 tgcggaaggg gacgacccac atccggaacc ttgacgtgca ccccggcgga ccctacgtga
      421 tctcctccgc caccttcccg acctacttca cccgcccaag gccgggaagc ccggacgacg
      481 cggcggcgga gctccacgcg gccctggacc tggaggtctt ccggacccac gtagccccgg
      541 tcctcggggc cgtcgagcgc ttcgtgggca ccgaaccgta ttgcccgacc acctcgacct
      601 acaataggct gatgagggag atccttccgg ccccgcggcc ggactctccc gccctggaga
      661 tccgggaact gccccgggtg gagcgggacg gggcggccgt cagcgcctcc cgggtccggg
      721 acctgatccg ggccggacga ttcgaggaaa ttcgaaccct ggtgcccgag accacctggg
      781 actggctccg ctcccccggg gcggcaccgg tcctggagcg tatccgggcc agcgacaccc
      841 ggcattgatc ggacggggcg gccggacggc cgccgaccgg ttttcccggg aggacgccgc
      901 agggctttcc ccgggtcccg cgaggttcgt atatgaggaa aggaagaata tgaaggccaa
      961 gaaggaagcg atggcgggct ccctggagtc cagcgacgtc ctggtcacca tccgcccccg
     1021 ggagggcgag ggcgtcgagt gcacgatcga gagcatcgtc ctcaagcagt acggccgccg
     1081 gatccgttcg gtcaccgagg aggtggcggc ggccgcgggc ctgtccgggg cggcggtgcg
     1141 ggtccaggac cggggagccc tggaatgcac cctccgggcc cgcctggaaa ccgccataga
     1201 gcggtcgacc gccggtacgt gagctccccg gccctggacg cccgggagga gcgctgggcc
     1261 cggcggctct ccctcgccgg gtccctgccc gcgggcggga gcctcctcac cctgaccctc
     1321 cggctccccg cggccctgcg ccttggcggg gatttcgacg gggccgcccg ggcgctgttc
     1381 gaggccctgg aagccgaggc ccgtacgtcg gggtcacccg tcctttcctc cgagttcaag
     1441 atctccgcgg acggccccga gggctggctg gccctgggga ccggcgcccg ggccgccaag
     1501 acgctcgccc tgggcgtgga ggaggaccat ccctggggta ccctggcgga catagacgcc
     1561 acggatgccc ggggccaacc tctcggccgg gaggacctgg gcctgccgcc ccggacctgc
     1621 ctggtctgcg ggggccccgc cgcggtctgc gccgccggag ccgcccacgg cctgccggag
     1681 cttcgcgccg ccgcggagac ggtcctggcc cggccggcct gcacgcccgg ccggataggc
     1741 cggctggccc tgcaggcact cctggaggag gcggccgtgg atcccaaacc cggcctcgtg
     1801 acgccccgct cccggggttc ccacgcggac atggactact tcaccttcct ggccggggcg
     1861 gcggccctgg ctccctggtt cgcggaagcg gccgacctgg gcgccgggct tgccgggttc
     1921 gcctctacgg aggaccccgg ggctcaagac ctcctggacc gtcttcgagc cgccggacgc
     1981 cgcgccgagg ccgcgatgtt cgaggccacg ggcggggtga acacccacaa gggactcgtc
     2041 ttctccctgg gggcactgtg cgccgcggcc gggtgcttgg cggcccgggg cttcgttccg
     2101 accgcggagt cctgctgcga cacggcgggc gccctggtcc gggccgccgc gaccgcggac
     2161 tttacggcgg cgtcggccaa acccgaggcg gagctgaccg cgggggagag actgtatcgg
     2221 agctcgggag tcctgggggt gcggggcgag gcggcccggg gattcccctc ggcgcttcgg
     2281 atcgggcttc cgcgtctccg ctccggcctg gccgccgggc tctcccggaa cgacgccctg
     2341 gccgaagtcc tcctggcgct ctgcgcggag gtcgaggaca cctgcgtcct ggcccggggc
     2401 gggccggaag gcctggagta tcttcgggcc gaagccgccc gggctctcgg cctgggtggc
     2461 ctatcgacct cggccggccg cgccgcggtc cggaatttag acgagggctt gatcgcccgg
     2521 cacctcagcc ccgggggctg cgcggacctc ctggcggtga cggacttcct ggagcgcctg
     2581 ggatagacgt ctccgcgccg gaccgcgggg gggacccgtg cggagcggaa gattccggca
     2641 aggccggttt atttcagatg gcgatccagg aagtccagga tacgggcgac attctccgga
     2701 gcctcgaacg cggttcctcc gtgtccggcc ccttccaagg cctggaagat caccttatcc
     2761 gccccgatct cggccgacag gcgggcggcg aagtcccggg actgggtgat cgggacgttg
     2821 cggtccgcgg tgccgtgctg gatgaacatc ggcgggtcat cggcggagat gtaggttccg
     2881 gggttcgcct tctcggccag gggccgggct tccggggtgc ccacagtctt ccccaggtac
     2941 ttcgactccg gggacgtcgg ggacgaggtc ggacccatgc ttccgctcgt acccagggcc
     3001 ttgaactgct cgtccatggc ggaaaaatcg agggggccgt accagtccac cgcggcccgg
     3061 acggagtcgg acactccggc gttccccagg gacgagtcca ccagggacgg gtcatccccc
     3121 gacacggcga ccagggccgc caggttccct cccgcggaac ttccccaggc ggcgaagcgg
     3181 cggggatcga ggttgtactt cacggccgag gcccgcagga atttcacggc cgccttcgcg
     3241 tcgttcaccg ccgcgggaaa cggggcctcc ccgctcagcc tgtaaccgac ggaggcgacg
     3301 gcgtaacccc gtttgagacc ctcgagcatg ggcccgatgc ccatggattt cccgcccacc
     3361 atgaatcccc cgggatggaa ctcgatgatc agggggaagg gcccggaccc ctcgttcgga
     3421 aggtacaggt ccagcttctg ggtgggggac ttggatgcgt aggccaggtc gagatgcttc
     3481 accttgatcc acgaagtgtc cgcggcctgc atgccctggg gttggacccc cgtcacgggc
     3541 tggcccgcgg aaagaaagcc ctggccggga acggacgggt cggcggtccc cgttccggag
     3601 ctgtccgcgg gtttcgagcc gcaggatagg atccagagcg tcgaagcaag gacgattggt
     3661 aatactttca gagttttcat ttttcctccc cccgatcttg ggatcggcgc ttctaaccat
     3721 agttcccggg attttggttg tcaaaaccga gcggagcgga ccgcggcagg tgcggaaaac
     3781 agggctgaaa agaggagcac cgacgaaagg gccaggacgg ctccacccgc gctctcggcc
     3841 ggtccgggga agagggaggt caccatcagg tagggacgcg aggccgccca cccgatcgcg
     3901 gcgatcccgg ccgtcccggc cgcggcccgg agaatccgac ccgcacggcc ctccggcgcg
     3961 aggggagcga agcgcaggcg tccccgggcg atcccggcag ccaagcggcc gcccgagtac
     4021 agagtcgcca gcgccaggac gccgaccaga acccacatcc ccgcgacgcc gaaccgtatc
     4081 cggctgaact tgaccggctt gaatccgcac caccgggacc agtcggccag caggacggaa
     4141 atgaccggcc agcttctctg ggtgttggtc aagatgacga tcccgtcccc ggattcgggc
     4201 accgcgtgga agtgggtcat ccagccgtgg ccctgcccgc cgtgccacac ggcccggcgg
     4261 ccgtcggaga gtcgctccgt gaagtggccg aacccgtagg cgtcggcggc catgccgaag
     4321 aggccccgga tctcggcgga atcggagtgg agggacccca gggagccggc cgtgaggact
     4381 ccggcgccct ccccgcccgg cacccgcatc ccggcacgga caaaggccgc gatgtcccgg
     4441 acggtggtga acaggccgcc ggaccccttc tcggaataca cgtaggctcc gaccctccgg
     4501 cccttgagat cgtggcccga ggcgctccgc tcccggattg tcgcgttcca ttcgtaggag
     4561 gaatccgtca tgcccagggg ctggaggacc tcctcggcca tatactccgc gaagggccgg
     4621 ccggacacgt tctccacgac gagctccagg aggttgaacc cgacgttgga gtactcgaag
     4681 ccggttccgg gttcccggtt catccggacc gtgccggcca gggcttcccg gagcgtgggc
     4741 ttgggggcac ccggcgcgta ttccaccgag ggacccaccg gacccaggac catccctgcg
     4801 tttccggata ggagggctcg gacggtgatc gtgtcttcct cgtaggggga gtccggaagc
     4861 cggaagtccc ccaggtaccg gctgaccggg gcgtccaggt cgatcaatcc gcgatcgacc
     4921 agggtcagga caccccaggc ggtgacggac ttggagatgg attcggcccg gaagaccgaa
     4981 tcctcgttca ggggacggcc ggagtcggcg atcccgtagg acttttgcca gacgattccg
     5041 ccgtcccgga tcagggcgat gcccaagccc ggcacctcgt accggcgcat taagccggag
     5101 atccgggcat ccagacggcg cgtgaacgac tctacggacc cgaccgcgac cgaggacgcg
     5161 gcgggcagaa gggcgagaag ggacaagaaa aacccggcga gaaggcggcg cgccgcaccg
     5221 gccgcaccgc cccctcccgg aaggctaagt atcatcggaa cgcccgctgg ttgcaggcgt
     5281 actgccaggc gtcggcggcg gcccgggagc cgaattcctc cgcatccgag cggagtccgg
     5341 acatccggtt cttcgcggcc cgtcccgcgg ctcgctcaac gcgggtcgtt cgggcggcct
     5401 ccgccttgcg caggaaagaa aggagattcg agatacgcag gatcaaggtc atggttcacg
     5461 ctccaatttc gttggacaaa ggaatccggg gcagcccgcg caaggatcgc gcgtggaata
     5521 ccccgaatcg cgtcggacgc gggagcggcg ggccgtccat ccgtcgtcga cgcatggatg
     5581 gccttcgggc gctcggccgc gcggccggga tccgtccggg catgaggaat cgccttgaaa
     5641 acctcgggac cggtgcttcc ccgacgccga caaagggcga agggtgtccg gatcgctcgg
     5701 tttcctgagg cgctcgggaa ggctgtctac ggttcagagt gcgcgatcca tccggatcgc
     5761 ggggcctccc gagctacatg cccagggcgg gagtggaggg gcagtaatcg cagaaaatca
     5821 tttagatact ccttgtacaa tcgcgcgaaa agagcggcat ccttccgccc tgcgaggaac
     5881 cgcttcgttc cccccatggg aaccggcgca tatcctcggg gaccatcccg gtgcggaccc
     5941 ttccgggctc cgcgtcgcac gattttccgg aaacgatatc gaagggaatc ggacgtgtca
     6001 agggtgtcgt gcaaaaaata tataatatcg agaacggttt cgaacttgct cccctccggc
     6061 ccggcggagg ggagccggcg tgtttgggct ccttacttct tcttggcctt cgaggcggaa
     6121 gccgtgctct tatccttcag ggccttgaac tccttggatc ccggcttgat cccgagttcc
     6181 ttggcgacga agccccaacc cttcgccttg tttttccggt agacatccac caccgtggcg
     6241 accggcttgc ccgagaggct ggaaagggcc gccacgaggt agacctccgc gggctggaac
     6301 ccctgggaaa ggacgacctg cacctgggtc ttgggaatcc cccactgcag gctgacctcc
     6361 gcggtgaaac ccgggaggtc gacccgtgcg gacgcgccga tctcgttcag ggacgcctcc
     6421 aggccggcgt cgccgagccc gaaggttatc gtctgggcgc ccgccgccgc cgcgaccagg
     6481 accaggatcc aacaaaccgc gcgtctcgcc atgatctcct cctgcccggg gacgccccgg
     6541 atacggtgag tatagcgcgg gatcaccccg cgtccaggcg ccggccggcc ccgattcccg
     6601 gagagaaggg acggaccttc ggcatcagac cgcagtcccc ctcgcctcgt tccggcttgc
     6661 cgccgcgagc gccgcggagc agacggccac gacgcaggcc agcaggaacc ccagggccgt
     6721 gaatcccgcg gaaaaaccgt gcaggtcgat ccaccggccc aggagggggg tgagcacacc
     6781 gctgccctcc atgcccgcga agaaatagag acccaggacg gtggaccgga ggttttccgg
     6841 cacccgaccc acgaggtagg cctccgcggc gggcatgcgc acgtagggaa ccgcgcccag
     6901 ggcggccagg agggcgaaaa acccgaatcc gtacggtacc cgggggaggg ccagcagccc
     6961 cgcgaccgcg gcgaaggccg cggccgcggc cacccgcttc cggccgaaac ggtccgccag
     7021 gcgcccgccc aggggagccg cccagacgcc ggcggaaaaa tacaaagcca ggagggcggc
     7081 cgccgtacgc tcggtagccc ccagaacgtc cacgatgtac agggtcagga aggaggaggc
     7141 cgaggccaag gccgccccca tccccgtcac catgaggatg aaggcggaaa gctccaggat
     7201 cgtccccggg gttagccgat ccctccggcc accgtccgcc ggacccgcga cggccggtcc
     7261 cccgagggag gcgcgcaggc acgcccggtg gatcaggatc cccagcagga cgacgggtac
     7321 cgagaaggcc aggaaggtcc cgcgccatcc caggacggca gccgcggcca cacccaggag
     7381 gggcgcggcc aggtagctga tgctgccgcc tacggcgtga aggcccaggg cgcgcccccg
     7441 ccgtttcggg tccaccgccg aggtgatcag gggggacgcc gcgggatggt accccccgcc
     7501 cagtattccc atgaggacca gggaggccag aagcagggag taccccgagg acaatcccag
     7561 gaggagcccc gcccccccga tgcccgcgat acccgcggtc agcacgaccc gcgccccgat
     7621 ccggtccgcc aggcgtccgg cggggatctg cccgaacccg taggagaggt tgaaggccgc
     7681 gagcacgacc ccgacctggg cataggacag ccccaggtcg tcccggatga agggcaggag
     7741 gggcgtcaac agggcggtca ggacgtggtg gctgaagtgc gccagggtca gtaggaggac
     7801 cgtccgggag gagtcgcgca tgcagccatt gtacggggaa tcgagcccgg agtccaacat
     7861 cgacttgcta gacggatacg gacgagagtc ccctcccgct tgaaccgccc atcgtcccgg
     7921 ggtagtattc gagcccctat gggcacaact cggcacggag gacccggggt atcggcgagc
     7981 ggccggtacc gggcgctcgg actgctccac atcatgaacg acggatggct cgcgagcctt
     8041 cccctgctcc tgcccttcct ccggggcgag ctcgggctgg actacgcggg tatcggcatc
     8101 ctgacgagcc ttctcggcgt cgccggaatc ttcctggccc tgccggccgc cggcctcgcc
     8161 cggcgtttcg gaggctttcg ggtgctcgcg gccgcggcgg tcctgtacgc ggcctccttc
     8221 ctggtcgtcg cggcggcccg gtcctacggc gccctcgccc tggccttcgg cctcgcgagc
     8281 ctcggtttcg gcgccttcca ccccatcgga ttttccctga ccgcctcggc gggtccgtcc
     8341 gcttccctcg ggaagcggat gggcggcttc accgccgtcg gggacatcgg gaggatcggg
     8401 gtcgccgccc tggtcaccgc gctcatggcc tggctgtcct ggcgccgggt cgccgccatc
     8461 tacgccgcgg tgccgatcct cgtgctcgca gcctgcctgg cgggcggtct gttccgggac
     8521 gcgaacgacg ccggaacaca cggattgccg gaaaccggga agaaccggag gttccggctc
     8581 tcggtggatc gccgcctcgc gcccatcctg gcgagcggct tcatcgacac cctcgcgggc
     8641 tcctcgctct tcgtgttcct gcccttcctc ttcaccctcc gcggcgcctc ccccgggctc
     8701 ctgggctccc tctcgggggc cttcttcctg gggaacatgc tcggcaaggt cgccctcggg
     8761 ggcaccgtgg accggttcgg gggccgtagg gtcttcttcg tctcggagtt cgtcatggcg
     8821 gccctcctgg tcgcactggg ccgctcggag agccttgcgg tcctggttcc gctctcggtc
     8881 ctgctgggcg cggtgaccaa gggaacggtc cccgtgatca acaccctgct cgcggaggcg
     8941 gccggggagg gcggcggggt cgaggaggcc ttcgccctcg gttccaccct gaacggcgtc
     9001 gcgggtgtga tctcgcccct gctcttcggc ctggtatccg aacggttcgg cgtggacaag
     9061 gtcttccccg tcagcgcggc cttcgccgtc ctcgcggtcc tgcccctgct cataacgagg
     9121 aaacggggag cggctcgaag ctgaaacgaa gggcgggaaa ccgaagcggg tctaggcccg
     9181 ctcgtccaca gagagctcgg cgacggcctt ggccatgagg cgatacgcct ccgcggccac
     9241 gcgggcatcc gccgcggaag gatctcccgg agcgcccgcc gcggccagga tcacgcgggc
     9301 ttttcggagg gtggcttcgg gatccccggg gacctcggcg aggtcgaccg cgatccggcc
     9361 gcccaccgcg gccggcccgt cgggagtcgc gaccgtgtcg tagagaaccg ccgacgcggc
     9421 gtagggtccg agggtggaaa gatgagccgc ctcgtgggcc cgcaccgccg cgtcccgcgc
     9481 gcgtaccctc tcggaattta tggcggggaa cccagcctcg gccggccttc cggccgattc
     9541 cccgttcccg gacggaccgt cctcggccag gcggaccacc agaaccggct cgctcgaccg
     9601 gccgccccgg acgacggaaa cggcgcggag ccccgggaca tgacccggga cgtcatagac
     9661 gatacgcatt cctggaaaga agtataggac cacccccgcg gctccgcaag ctcggcacag
     9721 ggcttggatg cccgggcaga gggttcgatc accggaacca cgcggccggg gcggggatgt
     9781 cgggcttgta gaccatcagc gcgaagacga cgacgagggg caggaccccc gccaggctga
     9841 tccaccaacg cagcctcagc aaccggatga gttcctccgg gagcgccgaa tcggcggggt
     9901 cgagtccggc catgaggcgt ttcagccggt actgggtcgg gatgtcgctg gccacccaaa
     9961 caaggccgga gagcaggaag aggacgaagc ccgacgccag ccagccgatc ccccacaggt
    10021 cgcccatcag gacgctgagc atcaggcccg cgacgaggga aaggaggatg aggggcgacg
    10081 agagccgcgc gtccagccag gccacggttt cgtaggtatg gaggatcacg tccttgcggc
    10141 ccgatgccag gctccggcgc tcccagagca tgcccacgac ggcgttggcg aagaacatcg
    10201 tggccgccgc gacgtggacg aacagggcgt ggcggtaggc gtcgggccgg ttcaggaggg
    10261 cggtgaactc cgcccggaac aagaccgcga aggcgaccag cccggccatg gcggccagga
    10321 agatccacag gacatgcttg gtcttcatgg tctaccattg gaacatagtt cccgttagat
    10381 tacaattacg gtcgcaaaca ccggcgagga gcggcacagg gatggaacca tgataaagaa
    10441 ggacatcgtc gtggtcggcg ggggcatcgc ggggcttacc gcggccctga gccttgcgca
    10501 caaggggaag gacgtcctcc ttctcgagaa aaacgaacgc tgcggcggcc tcatgaactc
    10561 cttcgtacgg gacgggttcc gcttcgaggg cggcgcgcgc gccctggtca acgccggcct
    10621 cgtgaagccc ctcatgaaag aattcgggat cgacctcgag gtcctgccca accccatcac
    10681 cctcgggatc gaggacagaa ccctcgtcat cgccggggag aaaagcctcg acgactacgc
    10741 ccgcttgctc aaggacctgt atccgggcag cgagggcgac gtggacagga tcatccgggc
    10801 cgtccgcgaa atcatcgagg acatgaaggt cctctacggc gtcgacaatc ccctgttcgc
    10861 ccagaagaaa aagaacattc tggttctcct gcccgagctc ctggcgtgga cgatccggtt
    10921 cttccggacc atgtaccaca tatcccaaat ggacacaccc ttcgaggagt acctggacgc
    10981 gatctcctcc aaccggtcgc tgaaggacat catcgggcag catttcttcc ggaagacccc
    11041 cgtgttcttc gccctgagct atttcgccct ctacaacgac tacatctacc ccaagggcgg
    11101 cgtgggggcg ctcatcgaca ccctggtcga cacgatccgg gcgcggggcg gcgagatacg
    11161 ctgcgggacg gaggtcaccc gcatcgacgc gaagacccgg atcctgacgg actcggccgg
    11221 caactccttc ggctattcga ggttgatctg ggcgggggac ctgaagactc tgtacgccct
    11281 gagcggggag gagggactgg cgcccaagga ccgggcctcc tacgcacgtc gcaaggaagc
    11341 ggtgctgggg caccggggcg cggagtcggt gttctccgtg ttcgcggccg tcgaccttcc
    11401 gcccgaaacc ttcgccaagg ccgcaagcgg acacgtcttc ttcacccccg accgacgagg
    11461 cctcgggacc atccataccg cagaggtccg ggacctgctc cggcgctggg acagcgtccg
    11521 gaaggacgag ctgttcgcct ggctgcgcag gttctgcgcg ttcaacacct tcgagatttc
    11581 gatcccggtc ctgcgcgatc cggacgcggc ccccccgggc aggacgggca tcatcgtcag
    11641 cgccctcttc gactacgagc tgaccgagag gatccgctcg tcgggctggt acgaagaatt
    11701 caagagccga atggaggacg agttcctcaa gacactgtcg tctagcctat acccgggtct
    11761 cgaggaaaaa atcctgttcc ggttctccgc ctcgcccctg tcgatccgcg agcgcgtggg
    11821 aagctcggag gggtccatcg tgggctggtc cttcgaggag gggatacccg ctgtgacgaa
    11881 catgttccgc atggccgatt ccgtgaagac cccgctcccc ggcgtgtgcg cagccggcaa
    11941 gtgggtctac agcccggccg gcggccccac cgccatcatg acgggcagga tcgccgccag
    12001 gcgctgtctg cggtaaggcg gcggccaggg ccggttcggg cccccgaagg ggtgaggcgg
    12061 agtttcgaat ttagagagca actcagactt gagcaccggc tactattccg gttcactcct
    12121 gtcccctggt ctgtcgatct ggacccggta tataccggaa cgaagtcagg aggaggtacg
    12181 atgaaagcac ctaggcggat cgtctctgtc gttctgcttg taatcttggc ttcctatatg
    12241 ataatcgcgc aatcgatcga ggccgactat cccggtgtga ggtcggatcc gctcgttttc
    12301 ccgccgccga cgggtcggga aaaagggttg atcgagagcg ggcgcacgga cgtaagtgat
    12361 taccaccccc tttccgactt cggcccggtt gccgatccat tcctggatga gatcgcggcc
    12421 aaggcgctct ggcatcgtcg acagggcgat ccggccaagg cggaggagct gtatgcctat
    12481 gtgatctggt attcggccac caacaacctc gcgctgacaa acggccaact caatggaatg
    12541 cttcagttac gggcattctc gctgtcccat ttgaaacgga acgaggctgc tgcccgagtg
    12601 ttcgacttca ggatcgcgat gctggaggat aggagttggc gtgagaggct ccccgcgcgg
    12661 cttgtcgtcc acgacgccct tgacgatcga acccgcgccg gattgctcgc cttcgcgtac
    12721 cgggatcgcg ccctcgagcg tacgatcacc cgtgattttg atggagcctg ggctgacgcc
    12781 ggggcttccg ccgccgagtt caacaagatc ggtgcgacgc gcgatgtctt gtatacctac
    12841 gaaatattca tcaccaacgc gaccttgctt gggggttacg atcatgtccc ggccgcgtat
    12901 gacgctttta ctaaagcctt ttcagggggc gggaaacagg aagcggatcg gatgcttcga
    12961 atttccggtt tcgcggctca agcgtatctc aaactcgggg acattgtcgg cgcctatgac
    13021 acgctcaacc tgatggtgaa gctttcggat ctctggggct acaaagatcc catggtcact
    13081 gagttccgtt cactccgtga tcaattgaaa cccctggtgg aggcgatgcc gaagcgtagt
    13141 cccggttcgc tcgtcgactt cctccgatcc ggcgacgccg ccgccgtgcg acggatcctg
    13201 gcggagggtg cggatccgaa tctcccggat gacacgaaca cgacgccgct gatgtacgcc
    13261 tgccgctccg gcggcagtat tgaaattgtg cgtttgctgc tcgaagcagg cgctgatccg
    13321 aacatccgcg gcgaaaatgg catcaccgca ctctccctgg caggaggctt caccagccag
    13381 gttgacatcg tccgagccct gctcgccgca ggtgcggacc caaacgcccg ctcgaaagcg
    13441 ggatggacga ccttgatgct cgtggcgcga aacggtacaa accccgaaat gctgcgggta
    13501 ctcctcgagg ccggtgcgga tccgaacttc cgaaccaacg acggagtaag cgcgcttcat
    13561 ctggcgagga attccggcaa caaggagata attcggctcc ttctggaagc gggagctcgt
    13621 tagggccgtt agtcaggcgg ggccgccgta aaagaggcgg ccgacttcta gggttctcgg
    13681 tttgccgtat tccttgaaca gcggaggagt gtttaggcaa atttggtttt caatccgctc
    13741 agtctaccgt ctctccccga tgatctggtt gtacgggacg aagatgttct tctccaggat
    13801 ctccttgatc cgttgccgat cccggacctt cagcgcctct atgatgcggg catggtcgtc
    13861 caggatggcc tctccgttct cgatgctctt ctcgccccgt cccatgaaga ctaggaggtt
    13921 ggacagcggg aattggtgga gcatctggat gaggcgacgg ttgccgctca tgttcaccaa
    13981 ggataggtgg aactgggcgt cgtgcacgat gcattccctg ggccgcttgc gtcggatcgc
    14041 cttggcgatc tgatcgttga tcttctccag gccgtccagg tcaccttccg gaaccgagct
    14101 tttcgcacat accagatcga cggccaagga ctccagcgcc caccggatct ggcagtattc
    14161 cacgatatcc tcgtcgtcca acctcgtgac gaaacacccc cgccggggaa cgttgtttac
    14221 gaggccttcc tgctcgagtt tatggaaagc gtccttgagg ggcgtcatgc tgacgcccag
    14281 gcgctcggca atcttttcgt agagcaggcg ctcgccgggt acataaaccc cggacaggat
    14341 atcgtccttg agcttggtga agatgcgttc gctcagtgtc tcgtttttca tgccttcccg
    14401 ccgtttccga cttcgtccac ccgtccggcg gactcagaac gccaggatat ctatacgccc
    14461 tcccgggccg ggttgtcaat cgaaatccaa gaaacatcga tcccatctaa cacaactgtc
    14521 aatataagat agaaatacaa atttaatttt ttataaaaaa taaattgaca gccgggtatc
    14581 ccgtggtatc atggcctggg atccaggagg aattcatgca caaggtctcg aagccgttcg
    14641 cgttctccaa aatgaaggac gcggaacagt tcgcccggga aatgcacgcc gcggggctgc
    14701 ccttccccgt ctccagcgac atgtccatcc tcggagagcc cgtccggctg ggcgccaaga
    14761 ccatcccgaa ccgcatctgc ctgcagcctc tcgagggatt cgacggcacc aaggaaggcg
    14821 cgccgtcgga tctcatcttc cgccggtacg accggtacgc ccggggcggg gcggggctca
    14881 tctggtacga atccataacc gtcgcggacg acgggcggtg caacccgttg cagatgatca
    14941 tcaagaagag cacgatcaag gagatacggc gcctggtgga ggaaaccgac cgaacggcct
    15001 acgaggcctt cggaaggaag ccctacaacg tcctgcagtt gacccactcg ggacgccgca
    15061 gcgtggacgc gaactggaaa tcgacgcccc tcgcggtagt cgccaatccc tatatcgacc
    15121 atcacaactc catagacggc caatccggga agatcactat cgcttcggac gagaagatcg
    15181 aacagatcat ccgggagttc atcgaggcgg ccgtgctgtc cgtggaggcg gggttcgatt
    15241 ccgtggacat caaggtttgc catgaataca tcctccggga actcctcgcg gccttcaacc
    15301 gcccggggaa gttcggcgga agcttcgaga accgcacccg ggcgcttttc gacatcatcg
    15361 atggcgtccg cgcccgggta ggagatgcaa tcgacatctg tgtcaggctc aacgcctacg
    15421 attgcattcc ctatccccac ggctggggca tggtcaagga ggagggggtc atggcccccg
    15481 acgtcagcga gcccatccgg ctctgtcaga tgctcgtcga acgggacgtc aagctcatta
    15541 acctctccac gatgatgccc cggtaccagc cctacggacg agggtacctg gcggaactcg
    15601 aggaggatgc tgaaattcaa ccttacaaag ggacgtacga gctcctgaag gcgacccggc
    15661 agatcaagga ggcggtcccg gaggggatct tcgtctgcac gggcctgacc tggctggagc
    15721 agttcggagg gaacgtaggg gccggcgggg tgcgggacgg atggttcgac atcgcggggt
    15781 tcgggcgcca ggccttcgcc tatccggact tcgcaagaga catcctgatc cggaacgtca
    15841 tggagcgtga aaagtgctgc atgacctgcg acaagtgcta cgacctcatc cagaagggac
    15901 acacggtcac gggctgcgtg atgcgggacc aggaggtcta tctgccgctc taccggaaac
    15961 atgtccagaa gcgggaggtc gaggcagtcc gctaggcgga ctgcccggac ggcctcctga
    16021 cgagaaagga gcgcgggaaa tgcattaccg gaaactgaac cgcacggacc tggaggtcag
    16081 tccgatctgc ctggggacgg tgaactacga caccgccctc ctttcaaagg ccgactccaa
    16141 gcggcagctc agccggttcg tggagagggg cggcaatttc gtggataccg cccacgtcta
    16201 cggcatgtgg gagccgggag cgacgagccg cagcgaggcg gcgatcgggg aatggatccg
    16261 ggagaccggg aaacgcgatt ccctggttct cgccacaaag ggtgcccacc cggaatgggg
    16321 taggatgagc gttccccgcg tccggccccg ggacatcgag gcggacctgg aagaaagcct
    16381 ccgtctgctc ggcacggatc acgtggacct gtacttcctc caccgggacg atccggcggt
    16441 gccggtatcg gacatcgtgg actgcctgga ccgggcccgg gaacggggga agatccgctg
    16501 gtacggctgc tccaactgga agctcggccg catccgggaa gccgcggcct acgcggcggg
    16561 gaagggctcc ccgggcttcg tcgtgaacca gctaatgcta agccttgcgg atatcaattt
    16621 ccacaatcta ccggataaaa ccttcgtcct catggaccgg gagacggcgg agtaccacgc
    16681 cgagaccggc ctcaacgtca tggcgtacat gtccatagcg aaggcctact tcacccggag
    16741 gcaccagggc gaaaagctgc ccgagagtgt gacctcggtg tacggcagcg agagcaacga
    16801 ccggatctac gagcgggccc gcgcggcggt ggacgcgggg gagtacacct tcatggacct
    16861 ctccctgatg tacctcatgg ccgagacgcg gtttcccacg gtgcccatcg cgtccttcga
    16921 cacgcctgag cagctcgagg agggcctgtc ctgctgggac aaacccgttc cgggcgccct
    16981 gctggcggaa ttgggacgga tgaagcggta cgtgtacttc ggatgaggat cccagcgcgg
    17041 atcggaaggg ccgggagagc gaaatgaagg aatccaggat acgggaaggg tacgagctcg
    17101 cgaaggaagc gtacggcgag ctcggggtgg acgtggacgc cgccgtccgg aaggcggact
    17161 cgatcccgat ctcgatgcac agctggcagg gggacgacct catcggcttc gacggggtcg
    17221 gcgagctcac cggagggatc gcggccaccg ggaactaccc gggccgggcc cgcaccgcgg
    17281 acgagctccg ggccgacctg gacaaggcca tgtccctgat cccggggacc aagcgggtca
    17341 atctccactc ctgccacgcg gagaccgcgg tacggaaggt cgaccgggac gcctatgccg
    17401 tggaacattt ccgttcctgg ctcgactggg cgggcgcccg gggaatcggg ctggacttca
    17461 atccgacctt cttctcccac ccccggatgg acggcgattt ctccctggcc agccgggacg
    17521 cgggaacgcg ggctttctgg atcgaacacg ggaagcgctg ccgagagatc ggcgcggagt
    17581 tcgggaaggc tctgaagagt ccctgcatcg tgaacttctg gatgcccgac ggctacaaag
    17641 acacccccgc ggacacggcc gcgccccgga cgcgcatggc ggaatccctg gaccgaatct
    17701 tcgagcggaa gttcgatccg gcccacctcc gggatgccct ggagagcaag ctcttcggcc
    17761 tgggcctcga gagctacacc gtggccagcc atgagttctc cctgggctac gccgtcaccc
    17821 ggaagatcct gtacaccctg gacgcggggc acttccaccc caccgagacg atcagcgcga
    17881 agctcagcgc cctcctgccc ttcgtgggag agctgctcct gcacgtcagc cggggcgtcc
    17941 gctgggacag cgaccacgtg gtctcctggg acgacgaact gcagaacatc atgaacgaga
    18001 tcgtgctgaa cgggtacgag aggcaggtgc gcatcggatt ggactatttc gacgcctcca
    18061 tcaaccgctt ggcctgctgg gtgatcggga cccggaatgc ccgcaaggcc ctgctcgccg
    18121 ccgccctgac ccccgtcgcc gcgatgcgga aggccgagga ctcaggggat tacacgaccc
    18181 ggctggccct gatcgaggaa gccaagtccc tgcccttcgg ccccgtctgg gatcactact
    18241 gcctggagag ctcggttccc gtcggctccg actggctgga cgagatccgc cgctatgagc
    18301 gcgaggtttt gtccgcccga ggccgggctt aggaggctac tgtggaaccg atcggatcga
    18361 ggacctggat catcccggac atgtactggc cggagcatac gtccccgggg cggtacgtga
    18421 gccacgaggc ggtctgcgta ttgaatccct cggacgcgga ctgcgaggtg gtcctcgacc
    18481 tctacttcga ggaccgcgcg cccattcgcg gcctccgggc cctctgcccg gccgcccgga
    18541 cccgccacat ccgcatggat gccttgaaaa gcccggacgg aagcccggtg ccccgggggg
    18601 tcccgtacgc ggccgtggtg acgtgctccg tccccgcggt tgtccagtac acccgggtcg
    18661 acacgaccca aagcgagaac gccctgatga cgaccctggc gtatcccgcc tcgggacggg
    18721 gttgagggcg gcgaggtacg ggaatgcgat actatctggg gatcgacaac ggcggaacgg
    18781 tcacgaaggc cgcgctgtac acgaaggacg gccgggaagc ggccgtggaa tcgcggagga
    18841 ccgaggtcct ctcccgggcc ccgggattcg ccgaacggga catggacgac atgcgcgaga
    18901 ccaacttcga ggtcatccgc tgcctgctcg agcgttccgg catccccggg cgggacgtgg
    18961 cggggatcgc ctgctgcggt cacggcaagg gcctttacct ctggggacgg gacggaagac
    19021 cggtacgccc cgggatactc tcctcggaca accgggcctg ggaatacccc gaacgctgga
    19081 accgggacgg aacggctccg aaggtgttcg ggaaaacctt ccaacgcatc ctggcctgtc
    19141 agccggtatc cctcctcgcg tggatgcggg atcatgagcc ggaaaaactc ccggagatac
    19201 ggtggatctt cgagtgcaag gactacgtcc ggttctgtct taccggagag gcgttcggcg
    19261 agctcacgga ctattccggg gcgaacctcg tgaacctcga gacccgggac tacgacccgg
    19321 aactcctcga gctgttcggg ctgtccggtt tccgggatcg ccttcccccc ctgcgttcgt
    19381 ccaccgacct ctgcggccgg atcacgccgg acgccgcgag acggacgggc ctggcggagg
    19441 ggaccccggt cttcggcggg atgttcgaca tcgacgcctg cgccgtggcg gtggacgccg
    19501 cggacgacga caccctctgc atgatcgccg gcacctggag catcaacgag tatgtccgga
    19561 gcgaaccggt ccgggacggc agcgtcatga tgaactccgt cttctgcgtt cccgaccgct
    19621 acctgatcga ggagagcagt cccacatccg ccgtgaacct ggagtggttc ctgtccaccc
    19681 tcaccccggg cgcggccgcg tcgaaagacg cctacgcggc cgcggaatcc tgggtggaaa
    19741 gcgtcccccg atccgacgcc tacccgctct tcctgccgtt cctgatggcc ggtgttccgc
    19801 atccgaacgc gaaagcctgc ttcctgggca tgacgtacta tcacacccga gcccacctcg
    19861 ccaaagcggt gtacgagggg atcgcgttca gccatcggta tcacgtggac cggctcctgg
    19921 cctcccgggg ccggccgttc gacaggatcc gcctggccgg tggagtgacc aaatccgccg
    19981 gctgggtccg gatgttcgcg gatgttctcg ggtccccgat cgagaccgtc gacatccgcg
    20041 agacgggaac gctcggctgc gccatgaacg ccgcggtttc gctcggagac tacgcggatt
    20101 tcggcgcggc cgcccgggga atgatccagg tccgcccggc ggtggaaccc gacccgtcga
    20161 agaccctgga atacgaccgg aggtaccgcc tgtaccgggc ggcgctcgac gccctggatc
    20221 ccgtctggga cggcatccag ggattgtacg acccgactcc ctagggatga aggtaccccg
    20281 ggggcgtccg acggcgcctc cggaccggaa ccgttgatcc caggcggaca cgcctgagac
    20341 aatagaccgc gcgaagcgcg acgaaggaga accgtcatgg gaaggaaatg gttgctgttc
    20401 gcagcgttgc tggctctggc cggatccgtg ttcgcccagg gaagcaagaa gatcacgatg
    20461 gtcggaatcg acaaggtcgt ctccgccaag gaattcggct acgacgccaa gccgaagggc
    20521 aagtacacca tcgcggtggt ggtgaagagc gccgccatcc cggtctggga atcccacctg
    20581 atcgccgccg agaaggcggg caagaagctc ggcgtcaaga tcatcgggta cgccccgacc
    20641 aaggcggaca acgtcgagga gcagaagcgg atcctcgagg acctcatcac taccggggtg
    20701 gatgcggtca tcctggcccc ggccaacacc gaggccgtcc gcggaccggt cctgagcctg
    20761 atcaaggccg ggattcccgt catctacgac aataccatgg gcccctccaa cgtggattac
    20821 ctgacctacg tgggcgtgga caacctcgag gtcgggaacg tcatcgccaa gaccatcggg
    20881 gacatgatgg gcggtaaggg caagctcctg gtcatggagg gcgtgcccgg acagtccacc
    20941 tcggacctcc gcacggaagg cgtcatgaag agcctcgcca agaactaccc ggggatcgtc
    21001 gcggaacgcg ccgtcaccaa gtggcagttc gacgagggcc ggaaggtcac ggaagactac
    21061 atcaccaagt ggggcaagga cctcaaggcg atcgtggcgg tcggcggaaa ccaggccgaa
    21121 ggcgccgtcg aggcggtcaa ggccgccggc atgaacggcc aggtcctgat cagcgggttc
    21181 gacgtgcagg aaccccagta caccgccgtg gccaacggag acgaagtatt cacggtatcc
    21241 cagggtgtgt acgatcaggc ctacctgagc gtggtggccg cgatccgtgc cttgaacggc
    21301 gagagcgtcc cccgcctgat caccaccccg atcacggtgg tgaccaagaa gaatctgacg
    21361 gccatggatg agcgccccgc ggccctgaaa gcccggtaaa cgcttccgcc cggcccgatc
    21421 gggccgggca tttcatttac cggatcaagc gaaccggact atcctgacca agcatccctg
    21481 gaccgggaaa gcgccgagcc tcccggtccg ggtctccgcc ggacctgccg gctgcatcct
    21541 cccagcgtga tagggatcga ccatgggaac tccagagtac ctgctcgaga tgagaaacat
    21601 caccaagacc ttcccgggcg tacgagccct cgacggcgtc agcttcgacc tccggcccgg
    21661 ggaagtccac gtccttctcg gggagaacgg cgcggggaag tcgaccttga tgaaggtcct
    21721 ggcgggggcc tacatccccg actccggcga gctgtacatc gacggccgcc ttgtggatca
    21781 cttcaacccg cggcacgccc agtccttggg tgtaggcatc atttaccagg aattcaacct
    21841 tgtgccctac ctgaacgtgg cgcataacat attcatagac cacatgcccc gcaccctcgg
    21901 agggctggcc ctcgaccatg cccgcatgca ccgggacgcg gaagccctat tgaaaagcct
    21961 ggacatgcgc gtcgacaccc gggccctggc ccacgacatc cccgtggccc agcagcagat
    22021 ggtcgaggtg gccaaggcgc tcacccacga cctcaagatc ctcatcatgg acgagccgac
    22081 cgcctcgttg tgcgaccggg agatcgagca gcttttccgg acgatccgcg agctcaccgc
    22141 ccgggggatc ggaatcgtgt acatctccca ccgccttcag gagatccccc tcatcgggga
    22201 ccggatcacc gtgctccggg acggcaggta cgtgggaacc gcgaacgcgg cggacctgac
    22261 ccaggaacgc atggtggaga tgatggtcgg ccgggaaatc agcaaccttt tcaaccggtc
    22321 ctatcgccct cggggggaac cggccctccg ggtgaaggat ctctcgtccc ggggaaccgg
    22381 actgagcaac gtgaacttga cggtacacag aggggagatc gtgggcttgg cggggcttgt
    22441 cggatccggc cgcacggagc tagcccgggc gatcttccat gtggaccccc acgaaaccgg
    22501 agagatcgaa ctcctcggca aatccctcgc caaggaagat ccgaagggcg cgatccgcag
    22561 gggcctcgcg ctgctgccgg aggaccggaa acgccagggg cttgccctga tcctctcggt
    22621 cgcggagaac gtcgtgatgg cgagcctttc tcggctcttt ccccgggggt tcctccggaa
    22681 ccgcagggaa caggaatccg tgtcgaaata cgtacaggaa cttcgaatct ccacgccggg
    22741 catacggcaa ttcgcgaaga acctctcggg gggaaatcag cagaaggtgg ttctggccaa
    22801 gtggctgtgc accgaggcgg aggtcttcat gttcgacgag ccgacccgcg gcatcgacgt
    22861 gggggccaaa caggagatct acgcgttcat ggatgatctg gcccgccagg ggaaagccgt
    22921 actcatgatc tcttcggaac tcccggaaat tatcgggctg tgcgaccggg tctatgtcat
    22981 gcgggaggga agcatcgtgg ccgagttgga cagaaccgag gcgacgcagg agacgatcat
    23041 cgcctacgcc atgggcggcg tgtgcgccga cgagggacgc gcgtcatgag cggattctcc
    23101 cgcatcctca agaacgtacc agcggtgctc tgggtggaac tccttctggt gatcgtgttc
    23161 agtttctttt cccggaatta cctgaccacc cggaatcttc tcatcctcct tcagcagggg
    23221 gccgtgctcc tggtcatctc cgcggcggcg accttcgtga tcatcagcgg gggcctcgac
    23281 ctctccctgg gagcgatcat gaccgtcgcg ggggtcttca ccgccctggc cgtgaacgcg
    23341 ggctttccca tcccggccgt catcgcggtc ggcgccctcg tcgggaccgc ctgcggggcg
    23401 gtcaacggcc tgctcatctc cttcgcgggc atggaaccct tcatcgccac cctgggcatg
    23461 cagggactcc tgtacggcat ttcgctggcc atgacgaaca aggagggcat cccggtctcg
    23521 aacgagcggt tcgtcttcct gggggacctg gtcaaccgga ccgttcccat ggccgcgatc
    23581 agctgcggcc tcatctttct gctggcggtt ttcgtgcaga accacaccct gctggggcgt
    23641 tacaccttcg cgatcggggg taacgaggag ggttcccgtt tgtccggcgt agagacccga
    23701 ttctggaaat gggcgatcta cgcgttcgcg ggttttctca ccgggctcgg cgcggtggta
    23761 ctggtcgccc gtctcgaagt ggccgacccc atcgtgggtg tccagtggga gttcgaggcg
    23821 atcgccgccg cgatcctagg aggcacgagc ctccggatcg gacgcggcga cgtccgaggg
    23881 acgatcgtgg gcgtcatgct gattaccatc atccggagcg gattgaacgt ggtacgggtc
    23941 ccttccgtct ggcagcccgc catcatcgga accatcatca tcctttcgat cgtgttccag
    24001 gtctggatct ccacctcggg aaaggaacgg aaatgacgaa gacttcttcc ggggtccgca
    24061 tccgggcgga cgcggccctg cttgcgggcc tgggacgctt cggggcgctg gcgatcctca
    24121 tcgtggtcgg agccttggcc tccgacgtct tcctgagcgg aaggaacatc agcaacatcg
    24181 tgaccaacgc cagcgtgatc atcatcctgg gcgtcggaca gaccatcgcc atcatcacca
    24241 acggcccgga tctctcggcg ggttcggtcc tgaccatttg tgcggtgatc gcgaccttga
    24301 tggtcaagca gcttggcata ttcttcccga tagccatcct ggccgccctc gcgttgggaa
    24361 tgttcctggg cttcctgaac ggctacatga tagcccgcat cggaatcccc tcgttcatat
    24421 ccacgtacgg gctccaatgg gccgtgttcg gattcgcgta cgtcatcctg aagggctacg
    24481 tgatctacga tttcgacacc tccttccggt tcatcggaaa cggatacctc ttcgggacga
    24541 tccccatgcc catcgtcgtg atgctcctgg tagtgacggg aggcatgttc atgctccgca
    24601 ggaccgtcgt aggccgtgaa ctctacgccg tgggcgcgaa ccgcgtcgcg gcccgcatgt
    24661 ccggcgtgga cacggagaag aagatcatac aagccttcat cctgagcgga gccctggccg
    24721 ccctggccgg gatcgtgctc gtggcacgca tcaacgcggt ccaggccgat atcggaaaga
    24781 actacgtcct gccggtcatc gccgcggtgt tcatgggcgg tacgagcccg acggggggcc
    24841 gcggaacctt gctcggaacg gtgatcggag cgctcatcat cacggtcgtg cagaacggga
    24901 tgaacctgct ggcggtggac agcgtctggc gggacgccgt gatcggcgtg ctgatcatcc
    24961 tgaccgtcct gatcgatatc acgatgcaga agaggctggc tcggatccag gccgcctgaa
    25021 atttatttta atcccgcgat tcctggcgct ggaatccctt ccgcaccgag taagactgag
    25081 gaacagaggt ggctgccgat ggacctgctc acggacctga ccttcctgca ctggttgacg
    25141 atcgccttgg ccgcggtact ggtcggttcg gcccgcgcgg gtctggatgg ggggcccctg
    25201 atcgccatcc ccctcatggc gggggtgttc ggcgcccgcc tgtcatcgtc gatcatgctg
    25261 ggcatcatgt tgaccgcgga cctcgccgcc ttgttcaagt acaggtccct gggaagcttc
    25321 cggcacctcc tccggaccct gccgtacgcc ctggcgggca tccttctcgg ggccttcgta
    25381 gggggacgcc tatccgagga gaccttccgg accgtggtgt ccgtgctcat cctgctgagc
    25441 gcggccatga tgctggccca agagctgaaa gccggcggct ttgtcctgcc cgagcggtgg
    25501 tattacgccg ccccgttggg gctgttgagc ggattctcca gcatggtcgg gaacgcgggc
    25561 ggtccgatca tgagcctgtt cctcctgtcc agcggcctgc tcaagaatca gttgatcggg
    25621 accatggtct ggttcttctt catcatcaac atgatgaagc tcccgttcca catcttcgcc
    25681 tggcataccc tctcggtccc gacccttctg gtcgacctcg ccgtggcgcc cctcgcggtc
    25741 gcctccgtgt tcggcagcgc ccggctagtc cagcgtatcc cggagaagcc gtaccggttt
    25801 ttccgcttgg ccaccgtggc cctcggcggg gcctatcttc tgttcacttg agcgggtctc
    25861 gaagtcccga ctccaggagg cgatgacatg acgatcgtcc gatccgacgc ggggggaacc
    25921 tacacgccgc ccggccacga cgcttccgta cgttcccgga aactcttcaa tccgtcgaac
    25981 ggatgtccca agctggacgc ccacgtgacg accttcgccc cgggcgcggg catggaggag
    26041 gagatccacg aggtttcgga ccatgtgttc ttcatgctcc ggggcgagct cgaggtgttc
    26101 cggggcgccg aggccgcggg cacgatcggt cccggggatg ccgtgcacat ccccgccggg
    26161 gaacctcatc ggctccggaa ttcggggcca tcggatgcgg tcttcctggc cgtgaccacc
    26221 cttccatgaa aaccgacgat cggccgcccc tgcctccccg gatcgggatc tacgagaagg
    26281 ccctgcccgc gggactcgac tggccgggga tgctccgggc ggcccgggag gcgggctacg
    26341 actacgtcga gatgtccgtg gacgagacgg acgcccggct ggcccgcctg gagtggagcg
    26401 ccggggagcg ggccgccttc cgcgaggccg tggcggactc cgggctccgg gtgccctcga
    26461 tgtgcctgtc cggccaccgc ctgtatccct tcggaagcgc ggatccggcg gtgcgggagc
    26521 gctcccggga catcatggtc cgggccctgg acctcgcggc ggatacgggg atccggacca
    26581 tccagctggc gggctacgac gtctactacg aaccctccac tcccgagtcc cgggagaagt
    26641 accgggaggg tctggccgag gcggtggagc tggcggaacg ggcccaggtc tgcgtggcca
    26701 tggagattat ggacaccccc ttcatgggga ccgtctcccg gtggctcgag ttcgcggagg
    26761 agctgccttc cgcctggttc caggtctacc cggacatcgg gaacctgtcg gcctgggccg
    26821 aggacgtgcc cggggagctt gcgaaggcca agggacgcat cgccgcggtc cacctcaagg
    26881 acaccctgaa accccggccg ggcttcccgg gtcggttccg ggaggtgccc ttcgggaccg
    26941 ggtgcgtgga cttccccggg gccttccgcg ccctgcggtc gatagggtat cgtggctcct
    27001 acctgatcga gatgtggacc catcagtccc cggacccggc cgcggagatc cgggccgccc
    27061 gacagtggat cctgggccgg atgcgggaag gaggcgtcgc gtagcccgcg gcgctcgctg
    27121 gcggcgcact tcgtgtgcgc cttccgctcc tactgtcgcg tactcgcgcc gatcaagtcc
    27181 agcagggagg ttatgtgaga gccccgcgac gcggggctcc tggacttgcc ctccgctccc
    27241 cggaatcctt ccggctcgct ccgggccgcc tcggggcact cgcgccgcgt cctgtggcgc
    27301 gtccgcccgc ggccctcaca tgatcgggcc gtgggtggat cgtgcccctc ggttcaagtc
    27361 caacagggcc cacggttgcc gggggctgcg gtgttactga gtcttatgtc acttattcag
    27421 ggaatttggg ccctgctgga cttgaaccag cgacctacgg attatgaggg ctaaggggcg
    27481 tggttacacc gagtagctct tggtacctcc agagtactct agactacagg atacatgtaa
    27541 ttctgactat ttcaagagtt aagaaaatga cagctcagga ttctcctgag ctgtcctgaa
    27601 ctacaccgag ctatctggaa ttggtttggc acattttttg tacagtgggg ggaatcgtcc
    27661 tggactctcc ctatctcgcc tgagctactg tgattccact cggggagtgt tccccagcag
    27721 ttcgtcgatc tccgcctggg tcattgcgcc cggggcattc cgatccgccg cttctgcctt
    27781 gtccaactcc gtacgcagca gggtttggag ttcaggatcc cggggcgcgc cgccgccctc
    27841 cagacgctcg gcgagggcgg ccgcgtccgt ccgatggaga ggatgccggg ccgcgaggac
    27901 cacgataggg cgggagcggc ggtccccgcg gatcgctttg accaggctcg aggcggatag
    27961 gccggaggga atcacgagcc cgccggacaa accgtcctcc ttgggcgcga agaggcccag
    28021 ggtcgaaccg ctccatgcgc cgaccacggt ggcgaccgcc caggactccc cgaggacggc
    28081 acagcgccgg acgaagcccc aggcttcgtc ggtggtaccg gcagcggcgg cccggacggc
    28141 gcgggcccgg agacacaggc cgctccagcc cggagacggt cccaggagat cttccgccgc
    28201 tctttccgga tcgtaccatg cggggacggc cgggttttcc ccggccccgg gatctccgaa
    28261 ggccgcgagg agcagatcaa tctccccagc ggaaaagacc tcggtgcccc gaatggagcg
    28321 cgggacggcg tttccgggaa aggcgggggt ttgccgggat cccaggaacc cagccttcca
    28381 ttcagctccc agggccgagc cctggtctgc ggaatctaaa accaaggcga aggctttctc
    28441 cgcatcgtag ggcaccgact cctcccggta gaggtggagc atgccgagta ggaagttcgt
    28501 gaagggaccc gcccgtcccg tgtcccggac ccgggataga aggtggaagg attccgacat
    28561 tgcgggaccg gcaagaaaac cgaaacggag cactccaagc agtccttccg tcccgccctt
    28621 ctccacttcg agggtcgaaa ggtaatcctg gaaggcccac tggaagggat ggatccggcc
    28681 gtaggcggat cgaaaccggt ccaggagatc caccgcttcg cccaggtccg ggagccccag
    28741 ggacagaagc cccccgatgt agaggctgtg ggcgtcggat gctccggctc cttcaacaat
    28801 ctcccgtaaa cccggttttc cgcccaggag gcgttcgtag tggaggggca tggttccccg
    28861 gatcagggcg tccgcgatgg aacgacaggg agatttccgc tccaggaagc tccggagttc
    28921 agattccgtg tcccgcgagg cggtttggag caacacggcg aagaaggtga acaggaggat
    28981 ccgggtcgcc tccccggaat ccttcccgac cgccgccccg agtccgaagg ccgccaggtc
    29041 gcccggggac ataccgtcga cggccaggga aagaagattt ccgagcacgg gatggtcgat
    29101 gacacgggcg aggtcttcga taaccaggag tccgcttcgt tcggcctcgg acttcagcgc
    29161 ccccaggggc ttcaagagga ccgggagggg ggcggaggcg aaggggacgg ctcggatctc
    29221 ttccgggctc aagaccatgc gttcctccta ttccttccga gaacgtactc gttgcaggtc
    29281 attccgtcac cgtgaaggtc gtccgggcgg acgccgaggc gggtggccag gattccggct
    29341 ccgtaatata gcggggtact gaagatccct gatcctgttt ccccgtgtcc ggaagaccgg
    29401 gatgcccggg ctcgaagggc cgggtggagg cgaaggccag gatggactcc ctgccgaggg
    29461 gaggtgtcag cttaaaggaa taccaatcgt ccacaggggg aaaacgttga tcagtaccgg
    29521 caggtatcca ttgtttccgg acgtggtcgc tcggccagat caatttggcc gtaccgtcct
    29581 cctggatgta gtagagccgg agccagaccg gccggtccac cgagaccagg atagtaattt
    29641 cttcgcctat ccggtacacg ccgccctcac cccggtcaac gtttacggaa agatggatac
    29701 tctccgcagc ttcggccagc gcctgtacga gccccggcgt gaaacccccg tctctcgccg
    29761 gatcaggggc ccaggtgcgc aggccgtcgg acctgtagag gaggcttccc agcgcgccgg
    29821 ggctggcgtc ccagcggccg tcgggggcga aggccgccca ctcgccccgg ccatcgacgc
    29881 tcaggtggac caaggggtca ccggtttttg gatcccgcgc ctctacaacg ccgtgatcga
    29941 gcattcgata ccggaggcgg ccgggctgta caagctcttc gactccgagg ctggttttct
    30001 ccacagccca ggacggagct tcatccttgg cggattcttt ccaggagccg gtcttcaggt
    30061 ccaggaagcc cgcggcgata acagtctcga taatcctccc gccgccgata tcccggccgc
    30121 cccggaaccc gctgatccag gcctccgtgc cggagttcga aatggccagc cttacgggat
    30181 gcagaggcac ctggtagcga ttatcagggc gttgaactgc gggggcggtc gcggagtcca
    30241 ggtgccacag gtggaggtag tgatcggagc ccctgggagc gtacgcggcg ccgatccgcc
    30301 cgaagtagtc cgtctcgaag agcttgggct cggaaccgtc gaggtcgagg atccgaccgg
    30361 gacggccggt tctcagatcg agcaggacca cggcgccctc ggccaggacc gccgcggaaa
    30421 gccgtccgac gacacggggc ggaaaaaccc gggaaaaggt ctttcggaga tccgagggaa
    30481 gcgggtcttt atccgcggcc gctgcccggg tcccgcccag gacgatgccg ggcggcaggg
    30541 ccgggacgcg cgtaagggtc ccgtcctccc cggtgccggg gccgcccggg tccaggcgga
    30601 acaggccttc tgaaccgtcg acccggcctc gaagaaagag aacgcgtcca gcaacaacga
    30661 gataggggcc ctccatctcg accctgtgca cggagcggac tcgccctgtc cggacttcgt
    30721 acaccttcag gatataccgc tccgtgacga acaccaggtc ctggtcggcg ctgaaggccg
    30781 cgccgaaagc ctgggcgttc ggggagagct cccggagcac caggcccgtg gaccgctcga
    30841 ggagtactac cggtccctgt tcggccagcc ccgcgacggc caagtctccc cgtggagact
    30901 cggcgaacca ggtctcaaaa ccgctcaggg tgcggtggaa gaggaattca ccagatcgtg
    30961 ccgcatcctg ggcaacggag gggaggtggg cggagaggag gaggaggagg aaggagatga
    31021 tcttacgatg gagaatcaaa gttcgactca tccaagcctc ctctcgcggg ccgcgggggg
    31081 atatattccc ccgctccccc cttcccgctt ttcttccgcc acgcctccgg cttcgcgcct
    31141 ccggtggaga gcgtaggcaa gcgcggtata attgccgcgc cagagcttga cccgtccgtc
    31201 cttgcggacg agtgcgtcat ggatgacaag cccgggtcag cgaatctgag gacgaacgag
    31261 acggaagccc aggtcctcgc cgctcccaaa gggagctctc tgtcctcgaa aggctacacg
    31321 tacaccaatc gcatcttgtt tccagccacc tccacgcgtc acacgcacat ccccagaggc
    31381 agcgccaacg gggtcgattt gagcggcatc actgtagctc cagccccagt cccaacacca
    31441 ctccgatacg ttcccactca tgtcgtacaa tcctagttcg ttgggaccat acgtacccac
    31501 gggcttggta ccatgtccac ccttcttatt gtacctggcc acagcgtcta cttcattccc
    31561 gccggcgtaa gtgtaacctc gactcgatac ccctcccctg gctgcaaact cccactccgc
    31621 ttctgtgggc aaccgccagc cgtccgcacc cttgttccaa gaaaccttat cctcgcttat
    31681 ggtgtacgct ggtgttaatc catctttctg acttaaccta ttcgcatagt ctatcgcgtc
    31741 gtaccagctt attttctcca ctggaaggtc atcgccctcg aaagaactag ggttcattct
    31801 catcaaagca ataaaatcct tctgcgtcac ctcagtcttc atcatccaga agcttgagac
    31861 agtcacagag tgcacaggct tctcgtttgg gcgaccggac tcgctgccca tccggaaagt
    31921 cccaccctga acaaagatct caccccggag gcttttgctt ttatctaggg atcgtgtggc
    31981 ttttggcatc ggtatatagg tgaaggacag agcggtcgct tcacccgcag ataccatccc
    32041 aacgtagctt tctacctttc cgtcctcgta tcgaactgtt acacagtggg ggccggcggg
    32101 caagtcgttc accggcacag tccccgcaga tacagtcata gtgacgttac ccacgatcag
    32161 ggtaccaggg ctggctagct tcagagacag gctgcccgat gtctcggtcg ccaccccaat
    32221 actaggagcg atggaatcga catatcggac gatgatcgaa ccagtcccat caaaataggc
    32281 gctaccactt gaatacgaac gtcctgtata ccagcgttta tgagtcgaat cgattctact
    32341 taaatggttt tcccgggcac ccataacacc tcgctccgag accgaataac tcaaactgga
    32401 actatccgga gcgaaattta ggttcattat tctctcaaat tgtccaaccc acatggctcc
    32461 atcaaatagt aagcaataac catcatcacc attactccta acaaaactcg gatcctttac
    32521 tataaaagcc aatcgacccc acctggggct tggatataaa tcaatatctt caggtcgaaa
    32581 tcctgtccct tttaagctgt cgcttacatc catgagaagg gagttattta ctattatttg
    32641 tattttatct ttattcttca cggccattag aaggtgggta ccatcaacag agatatgtgc
    32701 atctagaaca ttctcgtatg gccacaagca tccttcttct ttgccgaatt gcgtgatcaa
    32761 atcgcattta cgtccatcgc ggaatacata cacggctata aggtttccat tggaagaaat
    32821 tgagggatca aataagtcac cccaaatacc ggttgccgta tcatttctat aaagagtccg
    32881 cctcccattt tttccctcta cgatataagc gagagtgcca ttgtttgcta tagcgaatcg
    32941 tccaaaaata ttatgaaacg gcccgacggt tacggattgg ctacgtattc tccatccatc
    33001 gtccgacttg aacatgaacg catagttctt cccatccgga cttatggtaa gttgttcgat
    33061 ttccagatac ggccctgcga tagatcgtcc actagatgtg tacaagttcc aaacaggagt
    33121 cggcgacttg gtgtcgtcta tcctttttgc gctatataca agggtcttcc catccgaaag
    33181 aacccaatat tcccccatct cactaaaagg gccccacata cgagagccct cgaagctata
    33241 ccagcctttt tcattcttgg cttgtacgat aaaaccaact ccttggagat agcttacata
    33301 ccctatttca aggtatggac cgaaaacaga tgaacctaca tgaagataat tctttgaatc
    33361 agtagtacga acaaaggcaa actcgtcacc agatggggaa aaagtaccat tccatatata
    33421 tccggtcaga ggaccgtaga ccttgcctga aacatttata aagctggccc cactaaagtc
    33481 atcataagaa aaagcaagtt tttccccgtc gggcgaaagg cgcaaattat cagagcctat
    33541 ccatttataa ggcccctgcc gaggaaagaa attgctaaaa atcgaatagc cagccgtgct
    33601 ttttataaca gcaacatagt tcttcttcgc gccatataca tcgacgactg agctatccgg
    33661 aggtacagtg atcggataac tttgttcctc tggaaaagct tcaatatcga ataagaaaac
    33721 catgaccagg ataagtatcg ttttgattaa catatatact ccttctttaa taaaagtacg
    33781 gtatatactt ctagagatca aacctcgcat tgattataca ccttccatcc aaatccacaa
    33841 tttcttgaaa tttattagaa agggtatttg tactcccgcc cccgccggtg tatcctttta
    33901 tgcatgaaac cgaggtagtt catggattct gactggcaca accaggccga aaccctcttt
    33961 gaatccgcca cacgtatcgc ggcccagatc aggctcggcg gtactaccgg ccttagtatg
    34021 ctcccgaatc atgcgtatcc tcctatattc aacttgggcc tcgatatggc ccggaaaggg
    34081 gccgcacggg ccgatatcgt tgaagttatc gaaatactca tggcctcgag cggaggatcc
    34141 ttcgcgcacg ataatattaa ccgttttagc cttgctgcct tgctgctcgt cctggacggc
    34201 gaaggccccg acgccctgcg tgaagaacgc gaagcggcaa tccttggaag atccaggctt
    34261 ccgggtcgag accgtcgtcc gcccgtattc gaggtatcgc cggacgggat tgaatcccgc
    34321 ctcaattgga tcgacacacg ggtctcaagc cgacaggatc tcctcatcaa gggcaatgtg
    34381 agcgatgaac tgtccgtgga cagctcaggc tcgatccggg tcgtcgggca ggttaacgcc
    34441 gcacatatcg aggccgaagg cagcatcaag gtggaaggct gtgtatttgg caagggcagg
    34501 gctgttatcc ggtgcggggg agatttccat gcgatatcac tcgaacgggc ccgcttagaa
    34561 tccggtggag atgtccacct tggtgcgaca gtcgagccga tcgagttgac atgtaccggc
    34621 gacttgattt gcgaaggcgg acccggcgta atcctaggag gttcggtccg ggcgggtggc
    34681 tcggttcacg cagggatgat cggaggaccg ctatgtacgt gcacatcagt ttccgccgga
    34741 gccggtatca ccgtaactgg aaccatccac gaacgcacgg aaatctcggt gggacagtcc
    34801 cgcatgtccc tacgtcaagc tctcacgtgt gtcaggatac gggaagaagg gtttctcctg
    34861 cgactggagg ccgctgatgc tttttgacgt gtccgagata cgagcggctt cctgttccag
    34921 ggaggaacgc gccgtcttgg ctggtcttgc ggacaggatg gcggaaatgg ccgggacggt
    34981 agaaaaggaa ggactgttcg ctctgggcag gtacgccgag gaacatacgg gctttcttgc
    35041 ggaggccgtc ggctttgcgt tggaaggttc gggcggagcc aaaacggccc tccgggaagc
    35101 cgccttgtcc agcgggaaac ggggacgggc actccttgaa cgtctcatga tctcccaggg
    35161 tttagccgga atcacatcgg gcgagggtgc agacctagtt cgtctcaagg taagagccta
    35221 cacgggaacg gagttcttct tcagggaaga ccaggaagag gcaatccaaa gttttatgaa
    35281 tctcggtttc gaccgaagca cggcggagca gctttcaggt atcaactgag tcagatcacg
    35341 atttctgagt tcgggaaatg acagctcggg gcaaccctga gctgtcttga actacaccga
    35401 gctatctgaa agtggtttgg caaatttttt gtacagtggg gtcaacctca attcgaagtc
    35461 atagttcaaa gtctcataga caaatcaatt tctcattgaa tgatccctgt gtgcctggaa
    35521 ttagatatat cgcctatccg tgctcgtcgg gctcgggcta ttgaaatcac cgagtagcta
    35581 cctcattcaa ttccttcaca gttgacgcat tccatcacac atgaaggggc gtatttcggt
    35641 caggcaagtg tgaagcttgc agatttcaca cttgcgttct ttcctgggag gtctatactg
    35701 attcatcatc taaagatagc atggagaata ttatgagaaa gtcttggctg gcaatcggat
    35761 taatcgtagt ggttacgctg gtgttagagg cctgcggcac gacgcttcag gatcctgtta
    35821 aagcttcgtc aaagtctata acggcttttt ccttcgttgg aggggaggcg gccattatag
    35881 atcaggagca gcgtgtcatc cacgtcaatt tggactcgcg aataaacatg actggacttg
    35941 tcgccatctt cgagacgacg ggtgtgggcg tttctgtcga cggcgtcctt caactaagtg
    36001 gttcaacccc gaacgatttc tcctatcccc tgaggtactt ggttatagcc gaggacggca
    36061 cgatggccga ctaccttgta atcgtcgacc cacctctgat cgacgagtgt tgcattggtg
    36121 tcttagtaga ttcctttatg ggcgacattg attggagcgc agccaggaac gatatggtcg
    36181 attatgcatt catccgggcc cgtcaaggcg aattccctga cgccaagttc tccgccaact
    36241 gggcgggtgc tcgggctgcg ggtatcctgc gaagcgccta catatatgtt gacttcaata
    36301 aagacgtcga agaatctacc aatctattcc tcgccgattt taccggagat tacaaaagct
    36361 tcgaccttcc tcctctcatc ctgtgcgagg cggaaggcgc gacatcagag gcatatcgcc
    36421 ttaactggct tcagctctgc gtgggcttga tcgaagcgga gatcggcgtc aagccgatca
    36481 tcgctacggg aaccctattc tggtcgggaa gcatgggaaa ctcggacgcc ttcaccgagc
    36541 acccgctgat gatacgccat tatacgacgg ccattgtccc gaccttcccg tccgcctggc
    36601 ataactgggc tttctggaat tacaccggaa ccggtacggt taacggaata acgggagacg
    36661 tgacatcgat gctccgtctg gggtggacga aaaagcgttc cagccttttc ccgcacagct
    36721 tgccatgagg gcgaactgac cagcagctat aacctgtacc ttcggcgaca gatagtgcaa
    36781 ttatagtttt atatgattga gttcaaaatg acatcccatc cggtgagccg gattcttgat
    36841 tgctttcgat tgaacccaga cgaaggctct tcaattctaa ttgtggcaac tctcgcacgc
    36901 ttacactcat ctctcgtaga tgggctcgag tcacttcgct caatgagtct aaatcaccac
    36961 ccctgaaata cgtaagcgcg cgcaggcatt ccatttctgg gaattccgga tacagttact
    37021 ttgccgccgc gagacctagt tcgagggata caccggacat gcataaagcg gaaatatcta
    37081 aataatcctt agcctcgatt ccgtcaaata ggactgcaag ctatgtggcc atgagatcaa
    37141 tcggcgacgc aacaaccagg gctttatccg tcgtaatgga aggttcccct acacggctaa
    37201 agttaatggt cccgaaaaaa gaaagcttaa cgcggtcttc attcaccagg aaggaaaagg
    37261 tgttcggctc accttgaagt atgcttcaga tctcctactt gactacaatc catttacgta
    37321 tgacttcttt atccaaggaa ctctcggtga agaaatcaaa gtccttggac tcccggtgac
    37381 cacaatacaa cgcgacggtg gttccattgt acagcacgaa tcccatggcc tttgtccaag
    37441 cgagcgtggg gtacagcagt ttttgtgctt ctggtaatgt ctcaagatgc gggaccaccg
    37501 atctcaggga atgaccctac ttggaagcgc gggcaaggaa tgctcataga gcccgttcag
    37561 ccgatactgc caatacgccc aagatcttgg ccggaattga ccaggctccg cctgtgaaag
    37621 ggcatctcga agcccttctt cgcctatcag cgagagtaga agctggatgt cggagaaggt
    37681 ccctttatcc attatggcgg ctataacacg ctgtggatag gccagagact cctctggcgt
    37741 cttccaccag cagtacttcg ttgccaggtt cacgatcaca ggcagatgag gcaatgtatc
    37801 gattcccgat accgcgacat tgaagggaga attccttggt atggagctca tgccccctct
    37861 ccgatatctt tataagtcct ttttataaaa caatttgggc cctgctggac ttggcctaca
    37921 gtcgcgatcc tcctgatcgc tccttccggc ccgcctcggg gcactcgcgc cgcgtcctgc
    37981 ggcgcgtccg cccgcggccc tcactagatc gggctgcggg cggatcgtgc ccctcggttc
    38041 aagtccagca gggccgccgt gaagcaaggc ggccgacttc gaggtttaac ggtgtcttgt
    38101 actgggaatg ggccctgctg gacttgaacc agcgacctac ggattatgag ggcacagggg
    38161 cgtggttaca ccgagtagct cctggtacct ccagagcact ctagattaca ggatacatgt
    38221 aattccaatt atttcaagag ttcggaaaat gacaactcag g
//
LOCUS       DAOTEI010000011        37470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_742_length_37470_cov_5.583803, whole genome shotgun sequence.
ACCESSION   DAOTEI010000011 DAOTEI010000000
VERSION     DAOTEI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 37470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37470
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_742_length_37470_cov_5.583803"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="P5313_01885"
     CDS             <1..265
                     /locus_tag="P5313_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY79145.1"
                     /translation="SYDSAAIALKAIENAAIAAGKKMPARPDVAKAVRALKDFPGITG
                     TFTFNSKGDLEKAKYFIIQVTSGDPAKWSENKIDKILDIAPPQ"
     gene            332..1507
                     /locus_tag="P5313_01890"
     CDS             332..1507
                     /locus_tag="P5313_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY79146.1"
                     /translation="MRRLIKADALREILLPLGRMAIFVLGAGALLSVVMVVLSLLFRG
                     SGADKLLQTTQGVNIFTYTMANLPQVLIDGVTLGFVYAAIALGYTMVYGVLEFINFAH
                     SEIFALGAFAGVEFLIILDQTGVLKTAGGMSPYLYLVLALIVSMAASGGTAVFIERVA
                     YRPLRNAPRLVPLISAIGVSFALQDIIRLTESLTTGQFYRVIPTFGNFDDRIPLFSIP
                     TGGAPIRVDIQVKSLVVIAAAMFMLVGLNYIVNATRIGKAIRSVAQDRNTAAIMGIGV
                     NGIISFTFLLGGSLGGAAGVLYALKFTRIDPFVGFMPGMKAFTAAVLGGIGNLTGALL
                     GGIVLGMLESFAGAYLGTFTMGNFGSEYKDILAFLILIIVIIFRPSGLLGEQVSQKA"
     gene            1520..2938
                     /locus_tag="P5313_01895"
     CDS             1520..2938
                     /locus_tag="P5313_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY79147.1"
                     /translation="MKIQIRDALRKAAVFANRGKIPYILSLALLALGFILVYLSPRSI
                     PAFLVFAAAAALPYFLEIRKPVKLALAVLVAVVLVPILGARNIFYLEVIFQIAVFSAL
                     ALGLNIVVGFSGLLNFGYMAFYAVGAYLWAFFGSSQPFLLKAIPGTVPPGTPFFMAAD
                     WFYLFIFLGIILAAVVGLVLGLPVLRVRGDYLAIITLGFGEIVRQLANNLDKPINLTN
                     GPQGISPIQRPTLGPETVKFFNNIFEPLVGRPVTVNQFYNILFYLIALVVVVAAVVIT
                     YRLDDSKVGRAWTAIREDELAASAMGIPANKLKLGAFAVGASFAGAMGVLFAASRTFV
                     SPESFSFLQSVSVLTMVILGGIGSIQGVIIGASVVIILNLQVLQSFSLFLSRLRQSDA
                     YIPIINFYWRDLSNQLDPSKYQRLVFGIILILMMIYRPAGILPAARRKRELKAHVSED
                     GGTLAMNGNGGAASGTKEDDHA"
     gene            2931..3725
                     /locus_tag="P5313_01900"
     CDS             2931..3725
                     /locus_tag="P5313_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79148.1"
                     /translation="MPDSANILVAENVVKRFGGLVAVNKMNFNIEKGRLYSIIGPNGA
                     GKTTFFNAISGLYPPEEGAITFDGHRIERLVPEKITALHIARTFQNIRLFGGMTVVEN
                     IMVGAFTRLKQNPVQAVLRTKAFKEEEKRVEARAEELMEYVGLRGVGNELAASLPYGA
                     QRRVEIARALASEPKLLLLDEPAAGMNPAESEDAMKLFRRLRDDLGITILLIEHDMKV
                     VMNISERISVMDYGEKIAEGTPKEIASNPRVIEAYLGRGAASAMND"
     gene            3736..4446
                     /locus_tag="P5313_01905"
     CDS             3736..4446
                     /locus_tag="P5313_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79149.1"
                     /translation="MSLLELKEIHSYYGNIHALKGVSLTVEEGEIVTLIGANGAGKTT
                     TLRSISGLLHPKSGKVLFNGKDVTHLEAHLIHKEGIGMVPEGRGIFPRLTVLENLEMG
                     AYLVKDKAEIQRGVDRAFSLFPRLKERSSQKGGTLSGGEQQMLATARGLMSNPKILLM
                     DEPSMGLAPVLVDQIFDVIKELNSKGTTILLVEQNALMALSIAHRAYVLQTGSIVLSG
                     TAKEVSQDESVRKAYLGI"
     gene            complement(4545..5150)
                     /locus_tag="P5313_01910"
     CDS             complement(4545..5150)
                     /locus_tag="P5313_01910"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HRY79150.1"
                     /translation="MGRLSDWADGSGRFRIHTRSITTSTYPYDTNRVISVGRLTDTRH
                     APSYGFTGRPHPAGSLHDLEIILLLHTPDLTIEEVEVRIRAVPRPDCESLERSLDGAV
                     GLNIGPGFTEKAKALAGRGTGCTHLAHLLAVMAPAILQGWWALSDRERTEPDDARRRA
                     GSATLFLRDSCYTWRDGGNALRELAEIAGEGAVREEGTPGK"
     gene            complement(5336..6400)
                     /locus_tag="P5313_01915"
     CDS             complement(5336..6400)
                     /locus_tag="P5313_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79151.1"
                     /translation="MISKKDCLTVLTLLVVSLFITGCPTDVKLDTSIELSRLGRVYTA
                     GYSTGTIRAYYWVDDQLQTITEAGLGIATKAACVVPTTFGDYVAGARTDFVDPILIEH
                     AILWFNGKGEFLDDKPSFAFSAVLHDGKVFVAGWRNPDGSGDRPCVWEVRDSWMIVDS
                     GYVVSTRQIIRHDLPTDGGFGAARDISSDGSDLYVCGYYWDEGIRRACYWKNDERVDL
                     VDGNGSWAESLCVDEGKVYVGGKSASYACYWIDGNIYEFEDTTEFPAGASDFIVTAVS
                     VDEGELYLGGNYYKGDYLGFKWNAGTFSLSLPPVIGVGARGTDVYLGCEECWIKNDDD
                     TPLQWIGGTVRPRGIRMRLY"
     gene            complement(6529..7041)
                     /locus_tag="P5313_01920"
     CDS             complement(6529..7041)
                     /locus_tag="P5313_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY79152.1"
                     /translation="MRAGVRTDRDFLVRMGAAFAAIRETAGMSQSELAAATRLHVNTI
                     SNIERGRTDPSIFAVGLLCFGLGCPRIELADSGLVPRFDPSARLPPRVFRDLVWTPEA
                     VRVHSFSFRTCRLSAGLTLKDLSHLAGVHPNTVWNFENARAALSATAAYRLYRAVGAG
                     FIGGTEGVLN"
     gene            complement(7145..8263)
                     /locus_tag="P5313_01925"
     CDS             complement(7145..8263)
                     /locus_tag="P5313_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79153.1"
                     /translation="MKRTAVVLLIAASFLSPVHAEFSNFTFIAPRFNFRLLGGPPVPT
                     GADVEIRFPLSEAGGAPLALSLRLGAGYEDGKLVRDPAAGDPIQAPETFDDPAWFDAP
                     NAQWDIGVVLGWIRGSVGNRLEAFLLYRGRWDVYRGGFSTAVFPDARGLFGTALLAGL
                     GYDSVRDLGRGLDRGAAAEASVEWGPAFLASGFGPVEYIRFNASVRGYLPLVPERSAL
                     FPILAPQLSARVSADAAFGPDQPLFVLQGFGGRYEGRALAGEVRGYPTRAYDASLKAY
                     GNLELRVFGPALGPAAPVVLGFLDAGWYSGLHKSPTFGDASGTLASAGGGLALGIGPG
                     FHGGVYGGILVPGDDPLFPVYVPDGGRGFFSAMFTLHF"
     gene            complement(8334..10343)
                     /locus_tag="P5313_01930"
     CDS             complement(8334..10343)
                     /locus_tag="P5313_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY79154.1"
                     /translation="MKIRFGVRARILIPALTVTLLTLGIVIAVAYFVSTGIITEMAKK
                     EGDALAKEYAAELDAWLEVPMDEARALSRALLGMRRAGIRDREAYASVLKAALEGNTL
                     YSAAWTMWENGAFGDREAEFSGTLYGTESGRLALAWARDEKGGLERSGWVDAYEAEPF
                     YALPESSRGEVLLEPYPFSYAEGKPPVWMTSVCVPIVDQEMFIGVCGFDVGIQPLREL
                     VGGIKPMEEAYAILVDNQAKRISHPNADLIGKPVGDDTPLLKDALLGAIRDGKPYNLV
                     KKNLATGAVSYLAYAPVVVGADKSPWSLAVVLPLDKLLSGVDRLAIVLLGASGAGIAL
                     AVLVLLVSANAIVRPVTAARDAAVRFASGDLRRSGTGGEALAALALRRDELGEMAARL
                     DEAAGAVGTATSGVTAAARQVASGSEQVSSTAQTISSGASEQAASGEEVSSSMEEMGA
                     SIRQNADSAQTTEVIARKTAVDAEEGGRAVDEAVAAMKDIAGRIGVIEEIARQTNLLA
                     LNAAIEAARAGEAGKGFAVVASEVRKLAERSQKSAQEITELSSRTVGAAEKAGGIIRR
                     IVPDIRKTAELVQEIAASTREQSGGVEQVNKALGQLDTVIQQNASASEQLASMAEELS
                     SQSRLMLEALAFFRSDEDGTVEARAPAPARAAVSRTAIVPVEEED"
     gene            10514..14080
                     /locus_tag="P5313_01935"
     CDS             10514..14080
                     /locus_tag="P5313_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY79155.1"
                     /translation="MELSRGHGGVRPRFCFLLAGVHTGASVDLWHGVAEEAEARDIDL
                     LVLPAGRLGARDEFEYMRNGLARLIRPGSADGLLSWVSSLSGACGPEDLLALHEGFGP
                     VPLVTLAQRVGGAPVVSLDAYGGMRALLDHLRDVHGYERIAFLRGPELHASAADRFRA
                     YRDFLADRGLPYEERLVSSPLPWDEGGRALSELLDVRGLVPGQDFQAAAAASDLLAFW
                     FLRGLQDRGFQVPADVAVAGFNDSPESRLLAPPLTTVAMPFREQGRRGLSALADLWQG
                     ARKIEDAVLPVSLVVRESCGCPSREVLLAGGDSGPGPGMAGSGRRPGIDEAPGSPAPE
                     PSGPIEGPDSLDPAVEAVAEAAGADARAREAWVLPLVRAFRVEGDSGEDRFLPTLGRI
                     LDRMVHEEGPGLSWQNAVSALERLAGEGGARTARMEARALIAQARVMIGQAAARHSDF
                     RRWTAEEADKRLRSLGRALLTTYTVPGIAGALAERLPALGMLHAYLVLDRPGETPLLV
                     LALRDGLREDLPGDGLPVESPAELPADLLPPGRFTLVLEPLFVNEESLGCLVLDGRLG
                     KGTVFEELRGFVSAALQGARLFAQAAEARARAERADRLKTRLLANVSHELRTPLGMIQ
                     DEVRALDSAAGLPDDGRLVRGLARISSHARYQARVVNDLLDLSRADIEELDLAREYAD
                     PGVLARDCLESFRGGEGDGGSEVAWTADIREPLPLVRIDPVRIRQILFNLLENARRHT
                     ARGEIRLTVRADPPWILLEVSDTGSGIPPERLPEIFEPFSGDGEAGGIGLGLAISRRL
                     AALHGGRIDAVSAPGTGSRFTLRLPLPALGARIPSVEPARTWAAWVGTGSPDPKVVES
                     LRAAGWEVRRADTLTDELPGSGDPGPDALVLDPRAMDPASWALLRGLRRNPRLLELPV
                     LVYESAGDAGPAGGTPPPREILARLLDGASEDLLNLLGSPILAVDDDPAQRSRLRDFF
                     AREFPGFEVIEAARGDEALTLLAERVPALAVLDLGLPGASGEGILEFMKSEERLRHVP
                     VIVLTGRLLDPEELLRLDRHLRVVVQVKGIWTDEEFGRTVRLALRDELGAGNPAVKRA
                     LAWILAQYGRPFTRSRLAEAAGVNEDHLSRLFHRELGIGIWDFLNRFRMRKAKELLAA
                     TSLTVSEVADRVGFTDPSYFCRVFRKLCGATPQAYRESDGGR"
     gene            14168..15118
                     /locus_tag="P5313_01940"
     CDS             14168..15118
                     /locus_tag="P5313_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY79156.1"
                     /translation="MRGTKADRPKIPGRTFPETLSHAWKFRGIYALGILPLAFFLVIR
                     YLPLWNAQIAFKEFSPLDGVWGSPWNGLANFSAFFRSPYFWPLLRNTLMYSLAKLILG
                     IPAAVLLALALYETGRRALRKVVQTLAYLPHFLSWIILHGILLGLFSASEGLFNEINR
                     AAGVGTVNYMGDPAVFPFIVVLSDLWKEMGWSAIIFLAALMGIDPALFESAQVEGASR
                     LQQVRYITLPGILEVVVVVALLRIGTILDAGFHQLFVLYSVPVYDVGDIIDTWVYRQG
                     ILNADYSVATAVGLFKGLIGLGFLVAANRLAKRIAGSGLY"
     gene            15139..16011
                     /locus_tag="P5313_01945"
     CDS             15139..16011
                     /locus_tag="P5313_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY79157.1"
                     /translation="MRKSPGDAVFQFLVDNYLVVILILMVLPLWRVFMLSVTPQVDLS
                     DRTFGMLVPPSRWSLAAYKQFLTHPSFLVAAGNSFLILGMGVSLSLFLTVPLAYVLSV
                     RNLPGRRFLHTLVMIPFLFNPGLIPSYLVVTGLGLYDRLLAVVVPGAINVYNVFVMKN
                     FFEGLPEELKEAARIDGASELYILFKVVIPLSKPILLTIGLFYGVHFWNDFFNAILYL
                     GDARLQPLPILLRNILQAANFNEYVDIDVFSEARMESLKAASVFLTALPMLLIYPWIQ
                     RYFTKGTLSGGLKG"
     gene            16123..17685
                     /locus_tag="P5313_01950"
     CDS             16123..17685
                     /locus_tag="P5313_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY79158.1"
                     /translation="MKTNASRAFLSLALVLCVALGAGAQAKPVEIELWITGTASEAGP
                     PPADWAGYRIVRETLGIEMKISLLPTSFTDQDTKIVTAAAANRLPDILHVNRDVFIKL
                     TKQGLLAPVDELMKKMPTRTKTHYDDPLRNKLAMYKGKMYGLPDPGKMPMTDGWVIRK
                     DWLDKLGLPAPNTIEDFYKVAKAFTQNDPDGNGKNDTYGFGAYIETSDITNWGLGVRF
                     DPILGAYGVTGMWDVTSAKSFGLNVRKPEFYDAMTFVARLNKDGLIDPDWTTLKKDEF
                     RARWKQGKYGIMRENFAALSTVANYPAFDKNFPDGEWIPIPPPVGPKGKSSEGMLYVD
                     TRIHVVSKRAIDAGKGDAVAKLLEWFATDGYALAMFGEKGVNFTLDAEGNVSTAGLPD
                     PEKSYAKAANVPVLQLRNLASVNSDWELLPRYVPHKTINGRTIRPLDIWRYFTKQPWT
                     EATAALLIEPPANKADFERYYTENLVKFALGQQELSPASWKAFLAGLDKLGAKELEKS
                     AKAKVEEAGLLK"
     gene            17796..18989
                     /locus_tag="P5313_01955"
     CDS             17796..18989
                     /locus_tag="P5313_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRY79159.1"
                     /translation="MTPRVMDAPGAVGTAAFRVPGSVRWARTLLGRTPPETLSWHYEN
                     ALLLLAADRAGRVHGDPDLGTAARRFVDARVDGEGRIEGYREGEYNLDQINPGRLLLL
                     HPESGTPRLRTASMRLLDQLDRQPRCPSGGYWHKRIYPDQMWLDGLYMAGPFAAAWGL
                     RYGRPERFDEAVRQFRLAWERTRDERTGLLYHAWDESRRQLWSRPDTGCSPNFWGRAI
                     GWYAMALVDVLDYLPRDHEGRGILTEFLRRLAAAVLRYQDPESGLWHQVVDQGSRAGN
                     YPETSASAMFAYAFAKASRKGLLAGRDAEEAAASASRARAGIGRLMVREGPDGPVLEG
                     ICAVAGLGGDPYRDGSFEYYASAPRKPDDPKGTGPYILALLEAEARDDPDAAAALVRA
                     EEAVE"
     gene            18986..20302
                     /locus_tag="P5313_01960"
     CDS             18986..20302
                     /locus_tag="P5313_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HRY79160.1"
                     /translation="MSEDFARGGGYIDVAGALRSRAAAPDSDPASVRLLTRPLQALID
                     RASERGGGTLVFGPGTWRTGALVLRDDVGLRLEHGARLVASADPADFPVRAQRWEGRT
                     VPVHAPLIGAEGARRISVTGPGLVDGSGESWWTAFREGTLDFPRPRLVAFQDCRDVLL
                     EGFTAVDSPSWTVNPVRCERVEIRGIRIENPPDSPNTDGIDPDSCRDVRISGCSISAG
                     DDCIAVKSGTEDEEPRLRPASELIVITGCVFEAGHGGVVLGSEMSGGIRDVVVSACAF
                     RGTDRGIRVKTRRGRGGSVEGAVFSDLVMRDVPVPVAVNCYYGCGAWGDPVVADKGRR
                     LPDSGTPGVSRLRFAALQARGARYCAAFLYGLPEAPLDALSFSDCDFEIDPDWTEAGQ
                     PEMGDGLPDLGRAGFFARNVRDLELRSVRIRGHRGPEFDLGDPEPT"
     gene            complement(20295..21308)
                     /locus_tag="P5313_01965"
     CDS             complement(20295..21308)
                     /locus_tag="P5313_01965"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRY79161.1"
                     /translation="MKQYRVAVVGAMGAVGTEMIKTLEQRNYPVSELVPLDIPANAGK
                     TVTFRGAPVQVRAAQAGAFQGVDVAIFSAGADASKVLAPLAASEGAIVVDNSSQWRMD
                     PSVPLVVPEVNPQALDGHKGIIANPNCSTIQMLVALKPIHDAYRIKRVVVSTYQAVSG
                     TGGAAIAELTNQARQFTEGKPIAAKVYPRQILFNALPHIDVFLDNGYTKEEMKMVNET
                     KKILDPAIEVCPTAVRVAVYRGHSEAVNVQTERPFTLPDVRTLLEKAPGVQVMDDPSK
                     LVYPTALDADGKDAVYVGRLRLDPTVPNGLNLWVVSDNLRKGAALNAVQIAEVLIQRG
                     LLR"
     gene            complement(21542..21826)
                     /locus_tag="P5313_01970"
     CDS             complement(21542..21826)
                     /locus_tag="P5313_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79162.1"
                     /translation="MRRIEVIANRSVQEELIEALEHALPDFFYTVVPVVHGRGRQKRR
                     LGTSTWPEENFLLLAYVRDEDRTAVKDVVAAVKRKYPKEGIKIFSASADD"
     gene            complement(21826..24909)
                     /locus_tag="P5313_01975"
     CDS             complement(21826..24909)
                     /locus_tag="P5313_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY79163.1"
                     /translation="MSVAGRVIDRPVLISIVFALTAIVGLYTMGNLALDLMPESEMPR
                     LSVSTSYSRAGPESVEKGVTSVLESALINVNGLKSISSSSSEGSSRVTLEFNYGTDLE
                     DATNDIRDKLDRVRNSLPDGAGSPRIFRFDANAMPILRIAVSGKRSSEELKELAEDYI
                     APRFEQVDGIAQAEVTGGRSKIVRVEISQNRLDAYNLTLTGVASTLAGQNLELSGGTI
                     SEGSRNYLVRTTGEYRSLRQIADTVVADKGGYGVRLSDLGTVVEGYRDETQAVYIDGQ
                     PGVYVSLTKASGSNSVQAADGVYAKIEEIRGLLPDDVDLQIISDETKQIRDTISNLVS
                     SAYWGAALAMAVLFLFLRSLKSTVIVGLAIPLSMLVTLLAMFLAGITLNMMTMTGLIL
                     GVGMIVDASIVILENIHQYRERGAKPSVSAVLGSHDMISAILASNLTTVFVFIPVLFF
                     KDRLGMFGQLFSHMIFTIVIALLASLLVAVFLIPVLAGRFLPLSTREEKPLRNPVLRG
                     LDDATEGALRSLTRAYRRTLEAAIRHRPTTVLLVVGFLALSVAFLPRMNISLMPQSTD
                     DSVSLNVNLPVGTALSETKALLLDIQERAAAEIRGVRTMIATAGRGGSSYRAQLTIQL
                     PPFAQRIDSSDVVRNKLRSYFPDYPSASFSFSAGRFGGGGGSDIDLVLRVHDLAQGMS
                     TARSIVEILKAEVPDVAEPSISLTEGLPQVEVAIDRDRAYSFGVSVQAAASEISASLN
                     GYTATTYRTAGHEYDVVLMLREQDRSKVPDLQRIFVRGSAGRVPVANFASLGKSLGPV
                     SITRENQSRVIHVTANIVSDERPDRVEAKIRQAIAGALVLPEGITLSYEGSWKSISET
                     GQTFILIVTMALLLVFGVMAGQYESFKDPLINLFTIPLLVIGVVAVYLITGQPLSTFT
                     AIGVVMLVGLVVNTGIVLVDYTNLLVRRGMPVREACAAAGEARLRPVLMTTLTTILGL
                     CPMAFFPGENSSMIQPIGLTVIGGLVSSTFITLFFIPVMYTFFNEKRGHKKGEAA"
     gene            complement(24906..25976)
                     /locus_tag="P5313_01980"
     CDS             complement(24906..25976)
                     /locus_tag="P5313_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY79164.1"
                     /translation="MRIRNIVLLVFFSAAVALVLLLPKPKAAGAAGAAVSGGAPSAAA
                     GTAGQGPGGGARSGGPGAAGSSAAAGGSPAAAGPSGARAGRSASTTAFSVRTEPVRRG
                     TIQDYIESNGDVVSDSAVKVYPYIAGRLVSLKVPLGGRVSKGEVLAEVDPSTPGSAFS
                     LNPVLAPISGTVTSLPVAVGSLVGTGIAVAEIGLIEDVQVEARVPERFVGVLRTGLRA
                     SVSLEAYPDVTFPASVVRVSPVVDPVSRTKTIRLGFDRPDSRVNPGMFARIRLNTLAY
                     PDRLIVGEAALRSDAEGSFVFVVRDGTASKRRVVRGVAVDGRAEVVSGLEEGETVVIE
                     GAAALADGAAVRDIGTREGDRP"
     gene            complement(25979..27271)
                     /locus_tag="P5313_01985"
     CDS             complement(25979..27271)
                     /locus_tag="P5313_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY79165.1"
                     /translation="MQRFAILILAAAVAVGAARAQAAQPWSSAAPESAAALTPEAAVE
                     LALANNLALARSRVDLAAAERALSRAWNGAAPGITLSAGARRSSATEAVTLSGSAGVS
                     VTLAPRTLHEVRKARASRDVQSLAYETAARSLELEVRKSFYSLILARENLAVLRQSLA
                     TAQKGYDQVEAKRRAGLASELDALSARVNLENLKPSLESALTAYESQEARFKQTLGLE
                     QGAPISLVGSLAEASSVSALDFSGLSGEAPSVAALRASLESARVQEAAARSATRSPSL
                     VLSGSYGPQSVNSGPWTDNGSLSATLSFSLDNLMPWSSARETADKARDTTVKTESQLK
                     EALTGAEISVRSLRRSIEQSLASLAARRLTVEMAERSWRLTEEAYNFGTKDILSLQKA
                     GDNLQEARANLLKEAYNLVSALLDLEYALGLPFGTLGR"
     gene            27422..28123
                     /locus_tag="P5313_01990"
     CDS             27422..28123
                     /locus_tag="P5313_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY79166.1"
                     /translation="MKARVLIVEDVAELSDLVALYLAKEGMEARQAETAEAALALIPE
                     WTPDLIVLDLNLPGMDGFEFLHEFRKGGRCPVLIVSARDADEDIIAGLGYGADEFVTK
                     PFSPRVLTARVRALLRRAQDGTASRETPGLVRFGPFTLDTESCLLRKEGRRVPLSAKE
                     YAVLAFLAANPGRPLAPQSIYASVWKGAYGDPTAVAVYIQRLRRKLEEDPGDPRFLET
                     VHGMGYRFNPSGEPA"
     gene            28120..29514
                     /locus_tag="P5313_01995"
     CDS             28120..29514
                     /locus_tag="P5313_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY79167.1"
                     /translation="MRIRTQFYLLAAGIVIIPLAVMTGFYFAWRLREAPAPRIPGYEE
                     ILNLAGEPVDRAAWEELARFMERKPPTVEYTVLDPSMRVLFSTIEDFGAGEAVPDAEV
                     LDRVRGTGERYLYQLDSPVKTEGGRLLVLTRISREGRRPPSPFQRLFTTFATLLAALF
                     AVSAAGSVLIARSLTQSVLALEAATRRIAAGELDAPVAVRGGGELASLAASLVSMRDA
                     LKEDRTRRSRFIMGVSHDLKTPLALIKGYAEAIGDGMADAPGERERCVEIIGAKVDQL
                     DGMVDDLIDFVRMDTAEWRRHLRSVELAPFLRDLVRRTRADAELLGRRVQDEVELPEG
                     ISAPMDERLAQRALENILNNALRYTGPGGLVRLAARAEPGRAVVTISDDGPGIPGEDL
                     PRIFDLFYRGTASRREQGLGLGLAVVRGVADSHGWEIRVRSEPGRGSEFEISIPLSAR
                     PGSGTARRLSGLPI"
     gene            complement(29628..29954)
                     /locus_tag="P5313_02000"
     CDS             complement(29628..29954)
                     /locus_tag="P5313_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY79168.1"
                     /translation="MVIRNADVKKKDLFPGASRKILARGGKLMVVEVRFAAGSVVDAH
                     AHPHEQASYVVSGKLVLTMDGREEVLGPGDTFYAGPNVSHGVKFLEDSVVVDMFTPQR
                     EDFLQS"
     gene            complement(29963..31315)
                     /locus_tag="P5313_02005"
     CDS             complement(29963..31315)
                     /locus_tag="P5313_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HRY79169.1"
                     /translation="MKIAPYTGDMDSFDYLSLLVVDLQGALRSVSLPRSYVSDKVLAD
                     GIGFDASNFSFAQVHASDMIAVPDMASAFLEERGEFRVLHAFCDVRSTDGRPFLQYPR
                     VVARRAEEYLRTTGIGEQAHMLVELEFYVFEDVRYHTTVDKSYYFVESAEGLGEEHED
                     RDRFGLSKGYHRMGPDEKYGLLRDRAVRAMLEAGIPVKYHHHEVGASQMEIELDFMNL
                     SKAADGVVLAKWILKSTADELGLKLTFMPKPMYKIAGSGMHVHQYVTKGGKSIFPGEG
                     LYGLSEAGLAYTAGILSHARTGSLLAFSNPSTNSYRRLVPGYEAPVSATFAQGSRAAA
                     VRIPGYLKKGHARVEFRTGDATANTYYFLAAMLLAGLDGVRRNLDPVRLGFSSAKPSP
                     KTTFPTGLYHVLGGLKKDRDYLAPAFPEELLSNWIESKIKEAEYVYNAPVPQEYELYF
                     "
     gene            31542..32183
                     /locus_tag="P5313_02010"
     CDS             31542..32183
                     /locus_tag="P5313_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRY79170.1"
                     /translation="MNPVETWHAEALGAQAVKALQKNGFDAVYVATAAEAADKVLSYI
                     KPGASVGFGGSMTVKALGIQEKAAAKGAVLLDHNAPGLAPDKKLEILRRQLTCDVFVS
                     GSNAVTLEGDIVNVDGNGNRVAALTFGPSKAVVVVGANKIVRDLDEGFARVETYASPM
                     NNKRLDKPNPCVKTGTCEDCQGETRICRVYQILRRKPSLSDFTVVIVGENLGF"
     gene            complement(32315..32740)
                     /locus_tag="P5313_02015"
     CDS             complement(32315..32740)
                     /locus_tag="P5313_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79171.1"
                     /translation="MTKRFFPILAVLTALFLIASCASKPPVIPEDATAMELIQRAQEA
                     SDRSDWAAAIVYYETARERFGSDPSVLVTCEYETAFIHYKQGKYALAEEEFLALIAKY
                     ESPEGASLPPRYLILARKVLPTVREKMGKPAETTAESTN"
     gene            complement(32759..33073)
                     /locus_tag="P5313_02020"
     CDS             complement(32759..33073)
                     /locus_tag="P5313_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79172.1"
                     /translation="METLTELRESETADHAAARIYCANCVHCKLVPTPAHGTGRYYLR
                     VRCDAGKWKKKLGEEKIYKYFTVARRAIESCDAYEDMGDPREYMRDLKKSLPDKDEIY
                     TA"
     gene            33163..33861
                     /locus_tag="P5313_02025"
     CDS             33163..33861
                     /locus_tag="P5313_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRY79173.1"
                     /translation="MIATVVNAVAIILGTLVGLVLRKGFSEKMKDTVFTAAGCITLVL
                     GMQMAFKTGHVLALALALFIGGIWGTWMDVEGAVFRFGEVLKRRFAKGEEGSSFAYGF
                     LNASVLFCAGAMALVGSFRAGVEGDYSILLTKSVLDGFISVLFAGAMGVGVAFSALAV
                     FAYQGALTLLSVRIAHLVTETMLSELTAVGGVLVLMIGLNLLDLRKVRTGDFLPALLV
                     TVIFVLLFPYVPFL"
     gene            33905..35308
                     /locus_tag="P5313_02030"
     CDS             33905..35308
                     /locus_tag="P5313_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79174.1"
                     /translation="MSTNVANIRMARTLFQSASDQYYMLDNAAIIMPAISDLVATSLF
                     RIALVLDRPVDLPLLQRALANVAPRFPYFLVELRRGFFWYYFQPSRKPPAVIADLGSP
                     CQGFDPRKTRGHLFRVRARGSRIACEFSHILADGYGGLILAKTLLAEYFRLQGVASEP
                     APDVFPPGSEPNPHEWEDAYHRYFRENWPHPDPRPRAFRPPGPTLPLGQYRVLTGSFD
                     LAAVREAAQSRDATVTELLTAALMFAYQGIWESLPPKARRRRECMAAVEIPVNMRRFY
                     PSRTLRNFTLFILPAVDLRLGPYTFEELVRIVHHRVALENEEREIARQISRNAGGGRK
                     LLVRLVPLVLKNFFARLLYDSLGEGLITGLLTNLGSMTLPAPLDERVRRFEFVPAPSR
                     SLKTNAAVSSWKGELHVTFGSRARSREVERRFFSVLSSINLRARIECDADPTAPGVPE
                     PRPAESRTREGSWKPRD"
     gene            35290..36627
                     /locus_tag="P5313_02035"
     CDS             35290..36627
                     /locus_tag="P5313_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79175.1"
                     /translation="MEAERLNQKYANAARSDEFGPWYVLDNAAMIFPVMSDRVATYLF
                     RLAATLTEPVHLPSLSAALARIAPRFPYYMVDLHRGFFWNYLQPLNKTPSPVSDQRYP
                     VQDFDVRKRGQFPFRVRTRGRRIALEMHHALSDGGGALIFFKTLLAQYFRYRGIESDP
                     APDIFDPDGTPHPEEYEDAYNRFFQSELPVPEARPNAFRIVSPLLPPYQFRVTTGILP
                     VDKVKEVSKSFGVTVTELFCASIMDALQEIRASYPAWMRRRSGPMATLEVPINMRKIY
                     PTRTMRNFSLYFMPSVDLRLGRYDFGELAELVHYRIKSESPEREIPRQMARNVGISRK
                     RYVRLLPLGIKEVAMRAAYNSMGKRFMSGGVSNLGLLSLPEPLGSRVERMECLMPPSR
                     VTCGNLGAVTWGDRIYANFGSLMKSREVERLAFKRLVSLGIPVRLECNMEDPI"
     gene            36624..37352
                     /locus_tag="P5313_02040"
     CDS             36624..37352
                     /locus_tag="P5313_02040"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HRY79176.1"
                     /translation="MNLCPNCGVDLGDEARACPLCGVRVGPDADKPGPVIHPLPRMEP
                     LFDPDGKGNFSPREKLFITREVITVCLAIAAGSAAAINYLVSGHLTWSLYPVASIAFV
                     WLCVGFPLYVPKRPGLALTAAALGLVGFLGALDLIDGPEYTWFMALGLPLALCIEVFG
                     IGTVLAVIKARRKGLNIVSLILFGVTAICLSIEYLTDRFLRGEVHLQWSSMVAFALVP
                     VGIFLVYIHLRIATTTSLRKFFHV"
BASE COUNT         6017 a        12886 c        12372 g         6195 t
ORIGIN      
        1 gtcctacgac agcgccgcca tcgccctcaa ggccatcgag aacgccgcaa tcgcggcggg
       61 caagaagatg ccggcccgcc cggacgtggc caaggccgtc cgcgccctca aggacttccc
      121 gggcatcacg ggaaccttca cgttcaactc caagggcgac ttggaaaagg ccaagtactt
      181 catcatccag gtgacctcgg gcgacccggc caagtggtcg gagaacaaga tcgacaagat
      241 cctggacatc gcccccccgc agtgatccca gcctgattca atcccttccc cgcccccggg
      301 caaccggggg cggggacttc atggaggcct tatgaggcgc ttgatcaaag ccgacgccct
      361 gagagagatc ctccttcccc tgggacggat ggctatcttc gtcctgggag cgggagccct
      421 tctttccgtc gtcatggtcg tcttgtccct gctcttccgc ggttcgggag ccgacaagct
      481 tcttcagacg acccagggtg tcaacatctt cacctatacc atggccaacc tgcctcaagt
      541 cctcatcgac ggggtgacgc tgggcttcgt atacgccgcc atagccctgg gctacaccat
      601 ggtctacggc gtactggaat tcatcaactt cgcgcacagc gaaatcttcg ccctgggcgc
      661 cttcgccggg gtggagttcc tgatcatcct ggaccagacg ggagtcctga agaccgcggg
      721 cggcatgagc ccctacctct acctggtcct ggccctcatc gtgtccatgg ccgcctcggg
      781 agggacggcg gtcttcatcg agcgcgtggc ctaccggccc ctgaggaacg ccccccggtt
      841 ggtgcccctc atctcggcca tcggagtgtc cttcgccctc caggacatca tccgcctcac
      901 cgaaagcctg accaccgggc agttctaccg ggtcatcccg accttcggaa acttcgacga
      961 ccggattccc ctgttctcga ttcccaccgg gggagctccc atccgggtgg acatccaggt
     1021 aaaatccctg gtcgtcatcg cggcggccat gttcatgctc gtggggctca actacatcgt
     1081 gaacgccacg aggatcggca aggccatccg ctccgtggct caggaccgca acaccgcggc
     1141 catcatgggc atcggggtga acgggatcat ctccttcacc ttcctgctcg gcggttccct
     1201 gggcggagcc gcgggcgtgc tctacgccct gaagttcacc cgcatcgacc ccttcgtggg
     1261 tttcatgccg gggatgaagg ccttcacggc cgccgtcctg ggaggcatcg gcaacctcac
     1321 gggagccctc ctgggaggca tcgtcctggg gatgctggaa agcttcgcgg gcgcctacct
     1381 cggaaccttc accatgggaa acttcggatc ggagtacaag gacatcctgg cgttcctgat
     1441 tctgatcatc gtcatcatat tccgcccctc gggcctgctc ggcgagcagg tttcccagaa
     1501 ggcgtagggg aggtcgtcta tgaagatcca gattcgggac gccctccgga aagcggccgt
     1561 cttcgcgaac cggggaaaga tcccctacat cctgagcctg gccctcctgg ccctgggctt
     1621 tatcctggtg tacctgagcc cgcggtccat accggccttc ctggtcttcg ccgccgccgc
     1681 ggccctgccc tatttcctgg aaatccgcaa gcccgtgaag ctggccctgg ccgtcctggt
     1741 ggcggtggtc ctggtgccca tcctgggagc ccggaacatc ttctacctgg aggtcatctt
     1801 ccagatcgcc gtgttctccg ccctggccct gggcctgaac atcgtcgtgg gcttctcggg
     1861 gctcctcaac ttcgggtaca tggccttcta cgcggtgggc gcctacctgt gggccttctt
     1921 cggctcctcc cagcccttcc tgctcaaggc catccccggg accgttcccc cgggcactcc
     1981 cttcttcatg gccgcggact ggttctacct cttcatcttc ctgggtatca tcctggccgc
     2041 cgtggttggc ctggtgctgg gcctgccggt cctgcgggtc cggggagact acctggccat
     2101 catcaccctg ggtttcgggg agatcgtgcg ccagctggcc aacaacctgg acaagccgat
     2161 caacctgacc aacggccccc agggcatctc gcccatccag cggccgaccc tgggtccgga
     2221 gaccgtcaag ttcttcaaca acatcttcga gcccctggtg ggacggccgg tcacggtgaa
     2281 ccagttctac aacatcctgt tctacctgat cgccctggtc gtggtggtcg ccgcggtggt
     2341 cataacctac cgcctggacg actccaaggt gggacgggcc tggacggcca tccgggagga
     2401 cgagctggcg gccagcgcca tgggcatccc ggccaacaag ctcaagctcg gggccttcgc
     2461 cgtgggcgcc tccttcgcgg gcgccatggg ggtgctcttc gcggccagcc ggaccttcgt
     2521 cagccccgag tccttcagct tcctccagtc ggtcagcgtc ctgaccatgg tcatcctggg
     2581 cggcatcggg agcatccagg gggtgatcat cggggcctcg gtggtcatca tcctgaacct
     2641 ccaggtcctg cagagcttct ccctcttcct gtcgcgcctc cggcagagcg acgcctacat
     2701 ccccatcatc aacttttact ggagggacct gtccaaccag ctggacccct ccaagtacca
     2761 gcggctggtc ttcgggatca tcctgatcct gatgatgatc taccgccccg cgggcatctt
     2821 gcccgcggcc cggcgcaagc gggagctcaa ggcccatgtc tcggaggacg gagggaccct
     2881 ggccatgaac gggaacggcg gcgccgcatc cgggaccaag gaggacgacc atgcctgaca
     2941 gcgcgaacat cctggtcgcg gaaaacgtag tcaagcgctt cgggggcctg gtggccgtca
     3001 acaagatgaa cttcaacatc gagaagggtc ggctctacag catcatcggc ccgaacggtg
     3061 ccgggaagac caccttcttc aacgccatct ccgggctcta cccccccgag gagggggcca
     3121 tcaccttcga cggccaccgc atcgagcggc tggttccgga aaagatcacc gccctgcaca
     3181 tcgcccggac cttccagaac atccgcctct tcgggggcat gaccgtggtg gagaacatca
     3241 tggtcggggc ctttacccgg ctcaagcaga accccgtgca ggcggtcctc cggaccaagg
     3301 ccttcaagga agaggagaag cgtgtcgagg cccgggccga ggagctcatg gagtacgtgg
     3361 gcctgcgggg cgtgggcaac gaactggcgg ccagcctgcc ctacggggcc cagcgccggg
     3421 tggagatcgc ccgggccctg gcctcggaac ccaagctcct cctccttgac gagcccgcgg
     3481 cgggcatgaa tcccgcggag agcgaagacg ccatgaagct cttccgccgc ctacgggacg
     3541 acctggggat cacgatcctc ctgatcgagc acgacatgaa ggtggtcatg aacatctccg
     3601 agcggatctc ggtcatggac tacggggaga agatcgccga gggcacgccc aaggagatcg
     3661 cctccaatcc gagggtcatc gaggcctacc tgggccgggg cgccgcctcc gccatgaacg
     3721 attaaggaga agccgatgag cctgctcgaa ctgaaggaaa tccacagcta ctacggcaac
     3781 atccacgccc tcaaaggcgt ctccctcacc gtggaggagg gggagatcgt caccctgatc
     3841 ggcgccaacg gggcgggcaa gaccaccacc ctccgctcca tctccggcct gctccacccc
     3901 aagtccggca aggtcctttt caacggcaag gacgtgaccc acctggaggc ccacctgatt
     3961 cacaaggagg ggatcggcat ggtccccgag ggccggggga tcttcccgcg gctcacggtc
     4021 ctggaaaacc tcgagatggg cgcctacctg gtcaaggaca aggcggagat ccagcgcggc
     4081 gtggaccgcg ccttctccct cttcccccgg ctcaaggagc ggtcctccca aaaggggggg
     4141 accctctccg gcggggaaca gcagatgctg gccaccgccc ggggactcat gtccaacccc
     4201 aagatcctcc tcatggacga gccgtccatg ggactcgcgc cggttctggt ggaccagatc
     4261 ttcgacgtga tcaaggagct caactccaag ggcaccacga tcctcctggt ggagcaaaac
     4321 gccctcatgg ctctgtccat cgcccaccgg gcctacgtcc tgcagaccgg cagcatcgtc
     4381 ctgtccggca cggccaagga agtgtcccag gacgagagcg tgcggaaggc gtatctggga
     4441 atttagcgcc gggccgcgcg cggcccggcg gcccggaacg cttcgcgttc cgcgagtctc
     4501 cgtcaaatta agaagcgccg cccgggtctc accgggcggc gcttttattt gccgggagta
     4561 ccttcttccc gtactgctcc ctcccccgcg atctccgcaa gctcccgaag ggcgtttccc
     4621 ccgtcgcgcc aggtgtaaca ggagtcccgg agaaacagcg tcgcggagcc ggcccgtcgc
     4681 cgggcatcgt cgggttccgt gcgttcccgg tccgaaaggg cccaccagcc ctggaggatg
     4741 gcgggagcca tgacggccag caggtgggcc aggtgggtac atcccgtccc gcggcccgcc
     4801 aaggccttgg ccttctccgt gaacccgggg ccgatgttca gccccacggc gccgtccaag
     4861 gaacgttcca gggactcgca gtccggccga gggacggccc ggatccggac ctccacctcc
     4921 tcgatcgtca ggtccggcgt gtgcaggagc aggatgatct ccaggtcgtg cagggagccc
     4981 gccggatggg ggcgtcccgt gaagccgtag ctgggagcgt gccgggtatc cgtgagccga
     5041 ccgacgctga tcacacggtt tgtatcatag ggataggtcg atgtcgtgat ggaccgggta
     5101 tggatccgga aacggccgga cccgtccgcc cagtccgaaa gcctgcccat gttcgcccct
     5161 ccgaggaatc gggatggatc atgatgacat ggaaccgcgg atccgggcca ggagccagtc
     5221 ggacttgtgg atttcaagcg cttactggcg ctaaaaatcc cgactatccg tccgcctagg
     5281 agtttgtcgg gcttgcccgg gcggcggtct taggcttccg gttgacagtt actgatcagt
     5341 agagccgcat gcggattccc cggggacgaa cggttccgcc gatccattgc aaaggcgtat
     5401 cgtcatcgtt tttgatccag cattcttcgc agccgagata gacgtccgtg cctcgggcgc
     5461 ccacgccgat gacgggcgga agcgataggg aaaaggtccc ggcgttccat ttgaaaccta
     5521 gataatcccc tttgtagtag tttcccccga gatagagttc gccttcatcc acggagacgg
     5581 cggtcacgat gaaatccgag gctccggcgg gaaactcggt agtgtcttcg aattcgtaga
     5641 tattgccatc gatccaatag cacgcgtacg aggcggactt tcccccaacg tagaccttac
     5701 cctcatccac gcacaaggac tccgcccagc ttccgtttcc atcgactaaa tccacccgtt
     5761 catcgttctt ccaatagcaa gcgcgacgga ttccttcatc ccaataataa ccgcatacat
     5821 acaggtccga gccgtcggaa gagatatccc gggccgcccc gaagccgccg tccgtgggaa
     5881 gatcatgccg gatgatttgc ctggtactta cgacataacc gctgtccacg atcatccagg
     5941 aatcccgaac ttcccagacg cagggccggt cgcccgaacc atccggattc cgccaaccgg
     6001 ccacgaagac ctttccgtcg tgcaggaccg cggagaaggc gaaggagggc ttgtcgtcca
     6061 ggaattcccc cttcccgttg aaccagagga tggcgtgttc gatcagtatc ggatcgacga
     6121 aatccgtcct ggctccggct acatagtcgc cgaaggtcgt gggaacgacg caggcggcct
     6181 tcgtagcgat gcccaggccg gcttctgtga tggtctggag ttggtcatcc acccagtagt
     6241 aggctcggat ggttcccgtc gaatacccgg ctgtgtatac ccgcccgagc ctggaaagct
     6301 cgatgctcgt atcaagctta acatcggtgg gacagcccgt gataaaaagg gaaacaacaa
     6361 ggagtgtcag aaccgtgagg caatcctttt tcgatatcat gaaagctcct tttatgccgg
     6421 aactgcccgc cttcgcggga agccctgcgg cgttcaagga gactctagca cccggcgcgc
     6481 ccgggcaact cacaaggtat tgtgaaaaca ggccggtgtt tccgagcctt aattcaggac
     6541 gccctcggtt cccccgataa agcccgcgcc gacggcgcga tagaggcggt aggcggccgt
     6601 cgcggacagg gcggcccggg cgttctcgaa attccacacc gtgttcggat ggaccccggc
     6661 gagatgcgac aggtctttca gggtcaggcc cgcggacagt cggcaggtcc ggaaactgaa
     6721 gctgtggacc ctcaccgcct cgggcgtcca gaccaggtcg cggaagaccc tgggaggaag
     6781 ccgggccgag ggatcgaagc ggggcaccag cccggaatcg gccagctcga tccggggaca
     6841 gcccagaccg aagcacagca atccgaccgc gaagatggac gggtccgtcc ggcccctctc
     6901 gatgttcgag atcgtgttga cgtgaagacg ggtcgcggcc gcgagttcgg actggctcat
     6961 cccggcggtc tcgcggatcg cggcgaaggc cgctcccatt cggacgagga aatcccgatc
     7021 ggtccgcacg cccgccctca tggctgcatc gattccttcc cggtggatga tagggacgag
     7081 accacgggtt cgagtatacc acgggacccg gcttagctcc attcggcccc ggagccgctc
     7141 ggacttaaaa gtgcagggtg aacatcgccg agaagaagcc ccggccgccg tcggggacgt
     7201 agacagggaa cagcggatcg tcgccgggga cgaggatccc accgtagacc cccccgtgga
     7261 agcccggtcc gatgccgagg gccagccccc ctccggcgga ggccagggtg ccggaagcgt
     7321 ccccgaaggt cggggatttg tgcagcccgg aataccagcc ggcgtccagg aatcccagga
     7381 ccacgggagc cgccggaccg agggcggggc cgaatacccg gagctccagg tttccgtaag
     7441 ccttgaggga ggcgtcgtag gctcgggtgg gataacccct cacctctccc gccagagccc
     7501 ggccttcgta gcggcccccg aagccctgga gcacgaagag gggctggtcc ggtccgaagg
     7561 cggcgtccgc ggagacccgg gcggagagct ggggagccag gatcgggaag agcgcggacc
     7621 gctccggaac gaggggaagg taaccccgga ccgacgcgtt gaaccggatg tactccacgg
     7681 ggccgaaccc ggaggccagg aacgcgggcc cccattccac ggaagcctcc gcggccgccc
     7741 ctctgtcgag gccgcggccg aggtcgcgga cagagtcgta ccccaggccc gccaacaggg
     7801 ccgtgccgaa gagtccccgg gcatccggga agaccgcggt ggaaaagccg ccgcggtaga
     7861 cgtcccagcg gccccgatac agcaggaaag cctcgagccg gtttccgacg gagcctcgaa
     7921 tccatcccag caccacgccg atgtcccatt gggcgttcgg ggcgtcgaac caggccggat
     7981 cgtcgaaggt ttccggcgcc tgtatggggt ctccggccgc gggatcccgg accagcttgc
     8041 cgtcctcgta tccggccccc aggcgcaggg acagggccag gggagcgccg cccgcctcgg
     8101 acagggggaa gcggatctcg acatccgccc ccgtcgggac gggcgggccc ccgaggagac
     8161 ggaagttgaa ccggggggcg atgaaggtga agttcgagaa ttccgcgtgg accggggaaa
     8221 ggaaggaggc ggcgatcaag agtacgactg cggtgcgctt catcggctgc ctccgggggc
     8281 tgaataacaa tagtaaaata gtaatgttcc ccggaggcgc aggcaacagg tttctaatcc
     8341 tcctcttcaa ccggcacgat cgcggtccgg gatacggcgg cccgcgcggg cgccggggcc
     8401 cgtgcctcga ccgtcccgtc ctcgtcgctc cggaagaagg ccagagcctc cagcatcaac
     8461 cgggactggg aggagagctc ctcggccatg gaggcgagtt gttcgctggc ggaggcgttc
     8521 tgctggatca ccgtgtccag ctggcccagg gccttgttca cctgctccac ccccccggac
     8581 tgctcccggg tggaggcggc gatctcctgc accagctccg cggtcttccg gatgtccggg
     8641 acaatccggc ggatgatccc gcccgccttc tccgcggctc cgaccgtgcg ggacgagagc
     8701 tccgtgatct cctgggcgga cttctgggag cgctccgcga gcttccggac ctcggaggct
     8761 acgaccgcga agcccttgcc ggcctcaccg gcccgggccg cctcgatggc cgcgttcagg
     8821 gccaggaggt tggtctgccg ggctatctcc tcgatgaccc cgatgcggcc ggctatgtcc
     8881 ttcatcgccg cgacggcctc gtccacggcc cggcccccct cctccgcgtc gaccgccgtc
     8941 tttcgggcga tgacctccgt agtctgggcg gaatcagcgt tctgccggat cgacgctccc
     9001 atctcctcca tcgaggagga gacttcctcc ccgctggcgg cctgttcgct ggcccccgag
     9061 gagatggtct gggctgtgga ggaaacctgc tccgatcccg aggcgacctg ccgggccgca
     9121 gccgtgaccc ccgacgtcgc ggttcccacg gccccggccg cctcgtccag gcgggcggcc
     9181 atctcgccca actcgtcccg acggagggcc agggcggcca gggcctctcc cccggtgccc
     9241 gaccggcgca ggtcccccga ggcgaaccgc accgccgcgt cccgggccgc ggtcacgggc
     9301 cggacgatgg cgttggcgga taccagcagg acgaggacgg cgagcgcgat ccccgccccc
     9361 gaggcaccca aaaggacgat cgcgagccga tcgaccccgg acaggagctt gtccaggggg
     9421 aggacgacgg ccagggacca ggggctcttg tccgctccca cgaccacggg ggcgtaagcc
     9481 aggtaggaga ccgccccggt ggccaggttc ttcttgacca ggttgtacgg tttgccatcc
     9541 cggatggccc cgaggagggc gtccttcagg aggggcgtgt cgtccccgac gggcttgccg
     9601 atcaggtccg cgttgggatg ggagatccgt ttggcctggt tgtccacgag gatggcgtaa
     9661 gcctcctcca tcggcttgat ccccccgacg agctcccgga ggggctggat cccgacgtcg
     9721 aatccgcaga ccccgatgaa catttcctgg tccacgatcg ggacgcagac actggtcatc
     9781 cagaccgggg gcttgccctc cgcataggag aacggatagg gctccaggag aacctcgccc
     9841 cgggacgact cgggcagggc gtagaagggc tcggcctcgt aggcgtccac ccagccggag
     9901 cgctccaggc cgcccttctc gtcccgggcc caggccaggg ccagccggcc ggactcggtc
     9961 ccgtacaggg tgcccgagaa ttcggcctcc cggtccccga aagccccgtt ttcccacatg
    10021 gtccaggccg ccgagtacag ggtattgcct tccagggccg ccttgaggac cgaggcgtag
    10081 gcctcccggt ctcggatccc tgccctccgc atccccagca gggcgcggga gagggcccgg
    10141 gcctcgtcca tgggcacctc gagccaggcg tccagttcgg ccgcgtactc cttggcgagg
    10201 gcgtcgccct ccttcttggc catctcggtg atgatcccgg tggaaacgaa gtaggcgacg
    10261 gctatgacga tcccgagggt caacagggtg accgtcaggg ccggaatcag gatgcgggca
    10321 cggacgccga accggatctt cataggcggc tccttcggat caggatctcc gtacattctt
    10381 taccgtccaa gtatagtgaa tcgtcccgga acgcaagccc atatcgttga caataggccg
    10441 ggggggggta caatttcccg gacacgtacg gattccgcgc cgccggcggc gcggacgaaa
    10501 ggcggatcct gcggtggagt tgtcccgcgg ccacgggggc gtgcgcccga gattctgttt
    10561 cctcctggcc ggcgtgcaca ccggcgcctc cgtggatctt tggcacgggg tggcggagga
    10621 ggcggaagcc cgggacatcg acctgctggt cctgcccgcg ggccgcctcg gggcccggga
    10681 tgaattcgag tacatgcgga acggactggc ccggctcatc cgtcccgggt ccgcggacgg
    10741 tcttctgtcc tgggtttcca gcctctccgg cgcctgcggc cccgaggacc tcctcgccct
    10801 gcacgaagga ttcggtcccg tccccctggt gaccctggcc cagcgagtgg gaggggctcc
    10861 agtggtcagc ctggacgcct atggaggtat gcgcgccctc ctggaccatc tccgggacgt
    10921 ccacggctac gagcggatcg ccttcctccg gggtcccgaa ctccacgcct ccgccgcgga
    10981 ccggttccgc gcctaccggg actttttggc cgaccgcggc ctgccctacg aggaacgcct
    11041 ggtctccagc cccctgccct gggacgaggg gggccgggcc ctctcggaac tcctggacgt
    11101 ccgaggactg gtgcccggac aggacttcca ggcggccgcc gcggccagcg acctgctggc
    11161 cttctggttc ctccggggcc tccaggaccg gggcttccag gtccccgcgg acgtggcggt
    11221 ggcgggtttc aacgacagcc cggagagccg gctcctggct cccccgctga ccaccgtggc
    11281 catgcccttc cgggaacaag gacggcgggg cctgtccgcc ctggcggacc tctggcaggg
    11341 cgcccggaag atcgaggacg cggtcctgcc cgtgtccctg gtggtccggg agtcctgcgg
    11401 ctgcccctcc cgggaagtcc ttctggcggg cggcgattcc gggcccggac cggggatggc
    11461 gggctcggga aggcggcccg ggatcgatga agccccggga tcgcccgccc cggagccctc
    11521 gggaccgatc gaaggcccgg actccctcga ccccgcggtg gaagccgtcg cggaggccgc
    11581 cggggccgac gcccgagccc gggaggcttg ggttctcccc ctggtgcggg ccttccgggt
    11641 cgagggggac tctggggaag accgcttcct tccgaccctg gggcgcatcc tggaccggat
    11701 ggtccacgag gagggcccgg ggctgtcctg gcagaacgcg gtgtccgccc tcgagcggct
    11761 tgccggggaa ggcggggccc gaacggcccg gatggaggcc cgcgccctca tcgcccaggc
    11821 ccgggtcatg atcggccagg cagcggcccg gcacagcgac ttccgccggt ggaccgcgga
    11881 ggaagccgac aagcgcctgc gctccctggg ccgggccctg ctgacgacct acacggtgcc
    11941 cggcatagcg ggggccctgg cggagcggct cccggccctg gggatgctcc acgcctacct
    12001 ggtgctggac cgcccgggcg aaacacccct cctggtcctg gccctccggg acggcctgcg
    12061 ggaggacctg cccggggacg gcctgcccgt ggaatccccg gcggagctcc cggcggacct
    12121 tctccccccg gggcgcttca ccctggtcct ggaacccctc ttcgtgaacg aggagtccct
    12181 gggctgcctg gtcctggacg ggcgtctggg caagggaacc gtattcgagg agctccgggg
    12241 cttcgtctcc gcggccctcc agggcgcccg gctcttcgcc caggccgcgg aggcccgggc
    12301 ccgggcggaa cgggccgacc gcctgaagac ccgcctcctg gcgaacgtga gccacgagct
    12361 gcggacaccc ctcggcatga tccaggacga ggtccgggcc ctggattccg ccgccggcct
    12421 cccggacgac gggcgcctgg tccggggcct ggcgcggatc tcctcccacg cccggtacca
    12481 ggcccgggtg gtcaacgacc tattggacct ctcccgggcg gacatcgagg aactcgacct
    12541 ggcccgggag tatgcggatc ccggcgtcct ggcccgggac tgcctggagt ccttccgggg
    12601 cggggaaggg gacggggggt cggaggtcgc atggaccgcg gacatccggg aacccctgcc
    12661 cctggtccgg atcgacccgg tccggatccg gcagatcctg ttcaacctcc tggagaacgc
    12721 ccgtcggcac acggcccggg gcgagatccg cctgaccgtc cgcgcggacc ctccctggat
    12781 cttgttggag gtgtccgaca cggggagcgg gatccccccg gaacgcctcc cggagatctt
    12841 cgaaccgttc tccggggacg gcgaggcggg cgggatcggc ctgggcctgg cgatctcccg
    12901 tcgcctggcg gccctccacg ggggaaggat cgacgcggtc agcgcccccg ggacaggaag
    12961 ccgcttcacc ctccgcctgc ccctgcctgc gctcggggcg cggatcccgt ccgtggagcc
    13021 cgcccggacc tgggcggcct gggtggggac cggatccccg gatccgaagg tggtcgaatc
    13081 cctccgggcg gcgggctggg aagtccggag ggcggacacc ttgaccgacg aacttcccgg
    13141 ctccggggac ccgggcccgg acgccctggt cctggatccc cgggccatgg atcccgcctc
    13201 ctgggccctc ctccgcggcc tgcgcaggaa cccccggctt ctggaactgc ccgtcctcgt
    13261 atacgagtcc gcaggggacg ccgggcccgc gggcggcacg ccccctcccc gggagatcct
    13321 ggcacgcctc ctggacggcg cttccgagga cctcctcaac ctactgggca gcccgatcct
    13381 ggcggtggac gacgatcccg cccagcgtag ccgcctccgg gacttcttcg cccgggagtt
    13441 cccgggcttc gaggtgatcg aggccgcccg gggcgacgag gccctgaccc tcctggcgga
    13501 gcgcgtgccc gccctggcgg tcctggacct gggcctgccc ggcgcttcgg gcgaggggat
    13561 cctggagttc atgaaatccg aggaacgcct ccgacatgtc ccggtcatcg tcctgaccgg
    13621 gcggctcctg gatcccgagg agctcctgag gctggatcgc cacctccggg tggtggtcca
    13681 ggtcaagggt atctggaccg acgaggagtt cggccgtacg gtccggctgg ccctgcggga
    13741 cgagctcggg gccggaaacc ccgcggtcaa gcgggccctg gcgtggatcc tggcccagta
    13801 cggccggccc ttcacccggt cccgcctggc cgaggcggcg ggggtcaacg aggaccacct
    13861 ctcccggctc ttccaccggg agctcgggat cgggatctgg gacttcctca accggttccg
    13921 gatgcggaag gccaaggagc tcctggcggc cacctccctg accgtctccg aggtggcgga
    13981 ccgtgtcggc ttcacggacc cctcgtactt ctgccgggtc ttccgcaagc tctgcggcgc
    14041 caccccccag gcctaccggg agtccgacgg aggtcggtaa aatcctatag ggcgcctgca
    14101 tcttctcccg gaacgggtct accctgaagg cgagatgctc aagaagggcg gcggggggag
    14161 agtcgaagtg cgaggtacca aggccgatcg accgaagatc ccgggcagga ccttcccgga
    14221 aactctgtca cacgcctgga agttccgggg catctacgcc ctggggatcc tccccttagc
    14281 cttcttcctg gtgatccggt acctccccct ctggaacgcc cagatcgcct tcaaggaatt
    14341 ctcccccctg gacggtgtgt ggggcagccc ctggaacggc ctcgcgaact tctcggcctt
    14401 cttccgcagt ccctacttct ggcccctcct gcggaacacc ctgatgtatt ccctggccaa
    14461 gctgatcctg ggcatacccg cggccgtcct cctggccctg gccctctacg agacggggcg
    14521 tcgggccctg cggaaggtcg tccagaccct ggcctacctg ccccacttcc tgtcctggat
    14581 catcctgcac gggatcctcc tggggctctt ctccgccagc gaagggctct tcaacgagat
    14641 caaccgcgcc gccggggtcg ggacggtcaa ctacatgggc gatccggcgg tcttcccctt
    14701 catcgtggtc ctctccgacc tgtggaagga gatgggctgg agcgccatca tcttcctggc
    14761 cgccctcatg ggcatcgacc ccgccctctt cgagtccgcc caggtggagg gggccagccg
    14821 cctccagcag gtccggtaca tcaccctgcc cggcatcctg gaggtggtgg tcgtggtggc
    14881 cctgctccgg atcgggacca tcctggacgc gggattccac cagctcttcg tgctctactc
    14941 agtgcccgtc tacgacgtgg gagacatcat cgatacctgg gtgtaccgac aggggatcct
    15001 gaacgccgac tacagcgtag ccacggcggt ggggctcttc aagggcctga tcggcctggg
    15061 cttcctggtg gcggccaacc gcctggccaa gcgcatagcc ggcagcgggc tgtattgagg
    15121 aggccgaggg tgggcaggat gcggaaaagc ccgggggacg cggtgttcca gttcctggtg
    15181 gacaactacc tggtcgtcat cctgatcctg atggtcctgc ccctctggcg ggtcttcatg
    15241 ctctccgtga cgccccaggt ggatctctcg gaccgcacct tcggcatgct ggttcccccg
    15301 agccgctggt ccctggccgc gtacaagcag ttcctgaccc acccgagctt cctggtggcc
    15361 gcggggaaca gcttcctcat cctgggcatg ggggtgtccc tgagcctctt cctgacggtt
    15421 cccctggcct acgtcctctc ggtccggaac ctcccgggcc ggcgcttcct gcacaccctg
    15481 gtcatgatcc ccttcctctt caacccggga ctcataccca gctacctggt ggtcacgggc
    15541 ctggggctct acgaccgcct cctggccgtg gtggttcccg gggcgatcaa cgtctacaac
    15601 gtcttcgtca tgaagaactt cttcgagggc ttgcccgagg aactcaagga ggcggcccgg
    15661 atcgacgggg ccagcgagct ctatatactg ttcaaggtgg tgatccccct gtccaaaccc
    15721 atcctcctga ccatcgggct cttctacggg gtgcacttct ggaacgactt cttcaacgcc
    15781 atcctgtacc tcggggacgc ccggctccag cccctgccca tcctcctgcg gaacatcctg
    15841 caggccgcca acttcaacga gtacgtggac atcgacgtct tcagcgaggc ccggatggag
    15901 tccctgaagg ccgccagcgt cttcctgacc gccctgccca tgctcctcat ctatccgtgg
    15961 atccagcgct acttcaccaa gggaaccctc tcggggggcc tcaaggggta gcggcgttcg
    16021 atatgtattc gggacgaccg gcggaaaccg agtccgccgg gtcccggccc ggggccgtcc
    16081 ggtcccgaac aggttccgat cgcgttcctg aaggaggttg ccatgaagac gaatgcgtcc
    16141 cgcgcgttcc tctcgctcgc cctggtcctg tgcgtcgccc tcggcgccgg cgcccaggcc
    16201 aagcccgtgg agatcgagct ttggatcacc ggcacggcca gcgaggccgg cccgcccccc
    16261 gccgactggg ccgggtaccg gatcgtccgg gagaccctgg gcatcgagat gaagataagc
    16321 ctcctgccca cgagcttcac ggaccaggac accaagatcg tgaccgccgc ggcggccaac
    16381 cgcctgccgg acatcctcca cgtcaaccgc gacgtcttca tcaagcttac caagcaggga
    16441 ctcctggccc cggtggacga gctcatgaag aagatgccga cccgcaccaa gacccactac
    16501 gacgatcccc tgcgcaacaa gctggccatg tacaagggaa agatgtacgg tctcccggac
    16561 ccgggcaaga tgcccatgac ggacggctgg gtcatccgca aggactggct ggacaagctc
    16621 ggccttccgg ctcccaatac catcgaggac ttctacaagg tggccaaggc cttcacccag
    16681 aacgacccgg acggcaacgg gaagaacgac acctacggat tcggcgccta catcgagacc
    16741 tcggacatca ccaactgggg cctgggagtc cgcttcgacc ccatcctggg agcctacggt
    16801 gtgacgggga tgtgggacgt gacgagcgcc aagtccttcg gcctcaacgt ccgcaagccc
    16861 gagttctacg acgcgatgac cttcgtggcc cgtctgaaca aggacggcct catcgatccc
    16921 gactggacca ccctcaagaa ggacgagttc cgggcccggt ggaagcaggg caagtacggg
    16981 atcatgcggg agaacttcgc ggctctgtcc acggtggcca actaccccgc cttcgacaag
    17041 aacttcccgg acggggagtg gatccccatc ccgccccccg tcgggcccaa gggcaagagc
    17101 tcggagggca tgctttacgt ggacacccgc atccacgtgg tctccaaacg tgcgatcgac
    17161 gcgggcaagg gcgatgcggt ggccaagctc ctggagtggt tcgccacgga cgggtacgcc
    17221 ctggccatgt tcggggagaa gggagtgaac ttcaccctgg acgcggaggg caacgtctcc
    17281 accgcgggcc tgccggatcc cgagaagtcc tacgccaagg ccgccaacgt cccggtgctc
    17341 cagctgagga acctggcctc ggtcaactcc gactgggaac tgctcccccg ctacgtgccc
    17401 cacaagacca tcaacggccg gaccataagg cccctggaca tctggcgcta cttcaccaag
    17461 cagccctgga ccgaggcgac cgcggccctc cttatcgagc cgcccgccaa caaggcggac
    17521 ttcgagcgct actacaccga aaacctggtg aagttcgccc tgggccagca ggagctgagc
    17581 cccgcctcct ggaaggcctt cctcgcgggc ctggacaagc tgggggccaa ggagctggag
    17641 aagtcggcca aggccaaggt cgaagaagcc ggcctgctca agtaggatcc ggccggcaac
    17701 ccggaccccg gcggcgcgcg acgcgccggg gccccgggta cccgtggggc ggtccggcgg
    17761 tcccgggccg ctccgcgggc atcgagggag aacgcatgac acctagggtg atggacgccc
    17821 cgggggcggt cgggacggca gcgttccgcg tccccgggtc ggtacggtgg gcccggaccc
    17881 tcctggggag gacccctccg gaaaccctct cctggcacta cgagaacgcc ctcctcctcc
    17941 tggccgccga ccgggcggga cgggtccacg gggacccgga cctggggacg gcggctcggc
    18001 gcttcgtgga cgcccgggtg gacggcgagg gccgcatcga agggtaccgg gagggcgagt
    18061 acaacctgga ccagatcaac cccggacgcc ttctgctcct gcatcccgaa tccgggactc
    18121 cccggctccg gaccgcctcg atgcgactcc tggaccagct ggaccggcag ccccgctgcc
    18181 cctccggggg gtactggcac aagaggatct accccgatca gatgtggctg gacgggctct
    18241 acatggccgg gcccttcgcg gcggcctggg gcctgcggta cggacggccg gaacgcttcg
    18301 acgaggcggt ccggcagttc cgcctggcgt gggaacggac ccgggacgag cgcacgggcc
    18361 tgctctacca cgcctgggac gagtcccggc gccagctgtg gagccggccg gacacgggat
    18421 gctccccgaa tttctggggt cgggcgatcg gctggtacgc catggccctg gtggacgtcc
    18481 tggattacct gccccgggac cacgagggcc gcggtatcct gacggaattt ctccgccgcc
    18541 tggccgccgc ggtgctccgg taccaggatc ccgagagcgg gctctggcac caggtggttg
    18601 accagggctc ccgggcgggc aactacccgg agacctcggc ctccgccatg ttcgcctacg
    18661 cctttgccaa ggcctcccgc aagggcctcc tcgcgggccg ggacgcggag gaggccgcgg
    18721 catccgcctc ccgggcccgg gcgggcatag gccgcctcat ggtccgggaa ggcccggacg
    18781 gccccgtcct ggaggggatc tgcgcggtcg ccggcctggg aggggatccc taccgggacg
    18841 gcagcttcga gtactacgcc tccgcccccc ggaagcccga cgaccccaag gggacggggc
    18901 cctacatcct ggccctgctg gaagccgagg cccgggacga tccggacgcg gcggccgccc
    18961 tggtccgggc cgaggaggcc gtggaatgag cgaagacttc gcgcggggcg gcggatacat
    19021 cgacgtcgcc ggggctctcc gctctcgggc ggccgcgccg gattccgacc cggcctcagt
    19081 ccgcctcctc acccgccccc tccaagccct gatcgaccgg gcgtcggagc ggggcggggg
    19141 gaccctggtc ttcggtcccg ggacctggag gaccggagcc ctggtcctcc gggacgacgt
    19201 ggggctacgc ctggagcatg gagcccgcct ggtcgcctcc gccgaccccg cggactttcc
    19261 ggtccgggcc cagcgttggg agggccgcac cgtcccggtc catgcccccc tgatcggggc
    19321 ggagggggcc cggcgcatct ccgtcacggg accgggcctc gtcgacgggt ccggggagtc
    19381 ctggtggacg gccttccggg agggcaccct ggacttcccc cgcccccggc tggtcgcctt
    19441 ccaggactgc cgggacgtcc tcctcgaagg gttcaccgcc gtggactccc cctcctggac
    19501 cgtgaacccc gttcgctgcg agcgggtcga gatccgggga atccgcatcg agaatccccc
    19561 ggactccccg aacaccgacg gcatcgaccc ggattcctgc cgggacgtgc ggatttccgg
    19621 gtgcagcatc tccgccgggg acgactgcat agccgtgaag tccgggacgg aggacgaaga
    19681 gccccggctg cgccccgcct cggaattgat cgttataacc ggttgcgtct tcgaggcggg
    19741 ccacgggggc gtggtcctgg gatccgagat gtccgggggt atccgggacg tggtcgtctc
    19801 cgcctgcgcc ttccggggca cggaccgggg catccgcgtc aagacccgcc ggggccgggg
    19861 cggatcggtg gagggggctg tgttctcgga cctggtgatg cgggacgtgc ccgtgccggt
    19921 ggcggtgaac tgctattacg gctgcggggc ctggggggat cccgtcgtgg cggacaaggg
    19981 ccggcgtctc ccggactccg ggacgcccgg ggtgagccgt ctgcgcttcg ccgccctcca
    20041 ggcccggggc gcacggtact gcgcggcctt cctctacggc cttcccgagg cccccctcga
    20101 tgccctgtcc ttttcggact gcgacttcga gatagacccg gactggaccg aggcgggcca
    20161 gccggagatg ggggacggcc tcccggatct cggaagggcc ggattcttcg cacggaacgt
    20221 tcgggacctg gaactccggt ccgtccggat ccggggccac cgcggccccg agttcgacct
    20281 gggggacccg gaacctacct gagcaggccc cgctggatca ggacctccgc gatctggacg
    20341 gcgttcaagg ccgcgccctt gcgcaggttg tccgagacca cccagaggtt gaggccgttg
    20401 ggcaccgtcg ggtccaggcg caggcggccc acgtagacgg cgtccttgcc gtccgcgtcc
    20461 agggcggtcg ggtagaccag cttggaaggg tcgtccatca cctgcacccc cggggccttc
    20521 tccagcaggg tgcgcacgtc cggcagggtg aagggccgct cggtctggac gttgaccgcc
    20581 tcggaatggc cgcggtatac ggccacgcgg accgcggtcg ggcagacctc gatggccgga
    20641 tccaggatct tcttggtctc gttgaccatc ttcatttctt ccttggtgta cccgttgtcc
    20701 aggaagacgt cgatgtgggg cagggcgttg aagaggatct gccgggggta gaccttggcg
    20761 gcgatcggct tgccctcggt gaactgacga gcctggttcg tgagctccgc gatggcggcc
    20821 ccgccggtgc ccgagacggc ctggtaggtg gagaccacca cccgcttgat cctataggcg
    20881 tcatggatgg gcttcagggc caccagcatc tggatggtcg agcagttggg gttggcgatg
    20941 atccccttgt ggccgtccag ggcctggggg ttcacctccg gcaccaccag ggggaccgag
    21001 ggatccatgc gccactggct ggagttgtcc accacgatgg ccccctccga ggcggccagg
    21061 ggcgccagga ccttgctggc gtcggctccg gcggagaaga tggccacgtc caccccttgg
    21121 aacgcccccg cctgggccgc ccggacctgg accggggcgc cccggaaggt gacggtcttg
    21181 ccggcgttgg ccggtatatc caggggaacc agctcggaaa ccgggtagtt gcgctgttcc
    21241 agggtcttga tcatctcggt gcccacggcg cccatcgccc cgaccacggc cactctatac
    21301 tgcttcacgg tattcctcct gcgatcctat aggatatagg ctactcatca tatggtgagc
    21361 tataatatcg cgacttccgt caagtggcaa gagaaataaa tctcaggact caccacggag
    21421 ctcacggagg taagaagagg agcagaataa atcgccgaag ctagcttcgg cgccgtgatg
    21481 ccggcgctgc cggcatcacg ccctctgtgt ctctgtggtt cattcttggg aaaggcgttg
    21541 atcaatcgtc cgcggaagcc gagaatatct tgatcccttc cttggggtac ttccgcttga
    21601 ccgccgccac gacatccttg accgcggtgc ggtcctcgtc ccggacgtag gccaggagca
    21661 ggaagttctc ctccggccag gtggaggtgc ccagacgacg cttctggcgg ccccgcccgt
    21721 gcaccacggg caccacggta tagaagaagt ccggaagggc gtgctcgaga gcctcgatca
    21781 gctcctcctg gacggaccgg ttcgcgatga cctcgatgcg ccgcatcagg ctgcctcccc
    21841 cttcttgtgg ccccgtttct cgttgaagaa ggtatacatc acggggatga agaacagtgt
    21901 gatgaaggta gaggacacga gtcccccgat cacggtcagt cctatgggct ggatcatgct
    21961 ggaattctcc cccgggaaga aggccatggg gcaaagcccc aggatggtcg tcagggtggt
    22021 catgagcacg gggcggagcc gggcctcccc ggcggccgcg caggcctccc ggaccggcat
    22081 gccccggcgg accagcaggt tcgtgtagtc caccagcacg atgccggtgt ttaccacaag
    22141 cccgaccagc atgaccactc cgatggccgt gaaggtggac aggggctgcc ccgtgatcag
    22201 gtacacggcc accaccccga tcaccaagag gggaatggtg aagaggttga tcagggggtc
    22261 cttgaaggat tcgtactggc ccgccatgac cccgaacacc aaaagcaggg ccatggtcac
    22321 gatcaggatg aaggtctggc cggtctcgga tatgctcttc caggatccct cgtagctcag
    22381 ggtgatcccc tccggcagta ccagggcccc cgcgatggcc tggcgtattt tcgcctccac
    22441 ccgatcgggc cgctcgtcgc tcacgatgtt cgcggtgacg tggatcaccc ggctctggtt
    22501 ttcccgggtt atgctcaccg gacccaggct cttgccgagg gacgcgaagt tggccaccgg
    22561 gacccggccc gcggagcccc gcacgaagat cctctggagg tccgggacct tggaccggtc
    22621 ctgttcccgg agcatcagga ccacgtcgta ctcgtgtccg gcggtcctgt aggtcgtggc
    22681 ggtgtacccg ttcagggagg cgctgatctc ggaagccgcg gcctggacgg agactccgaa
    22741 ggaatatgcc cgatcccggt cgatggccac ctccacctgg ggcagaccct cggtcaggct
    22801 gatggagggc tcggcgacgt ccggcacctc ggctttcagg atctccacga tgctccgggc
    22861 cgtggacatg ccctgcgcga gatcatggac gcgcaggacg agatcgatgt cggaaccccc
    22921 gccgcctccg aaccttcccg cggagaagga gaacgaggcg ctggggtaat cggggaaata
    22981 cgatcgaagc ttgttccgca ccacgtcgga ggaatcgatc cgctgggcga aggggggaag
    23041 ctggatcgtg agctgagccc ggtaggaact gcctccgcgt cccgcggtcg cgatcatggt
    23101 ccggaccccc cggatctcgg cggcggcccg ttcctggatg tccaggagca gggccttcgt
    23161 ctccgacagg gccgtgccca ccgggaggtt cacgttcagg gaaaccgagt cgtccgtaga
    23221 ctgcggcatc agggagatgt tcatccgggg gaggaaggcg acgctgaggg ccaggaatcc
    23281 gacgaccagg agcaccgtcg tgggccggtg gcggatggcc gcctccaggg tccggcggta
    23341 ggcccgggtc agggatcgca gggctccctc cgtggcgtcg tccaggcccc ggagcacggg
    23401 gttcctcagg ggcttctcct cccgggtgga caggggcagg aaccgtcccg ccaggacggg
    23461 gatcaggaag acggccacca ggagactggc cagcagggcg atcacgatgg tgaagatcat
    23521 gtgggagaag agctgcccga acatccccag gcggtccttg aagaagagca cggggatgaa
    23581 gacgaagacc gtcgtcaggt tggaggccag gatggcggag atcatatcgt ggcttccgag
    23641 caccgcggag acgctcggct tggccccccg ctcccggtac tggtggatgt tctccaggat
    23701 gacgatggag gcgtccacga tcatccccac ccccaggatc aatcccgtca tggtcatcat
    23761 gttcagggtg atcccggcca ggaacatggc caacagggtg accagcatgg acaggggaat
    23821 ggccaggccg acgatcacgg tgctcttcag gctccgcagg aagaggaaga ggaccgccat
    23881 ggccagggcg gcgccccagt aggccgagga gaccaggttg gagatggtgt cccggatctg
    23941 cttggtctcg tcggagatga tctggaggtc cacgtcgtcc ggaaggagtc cccggatctc
    24001 ttcgatcttg gcgtagaccc cgtccgcggc ctgcacggag ttggatccgc tggccttggt
    24061 cagggagacg tacaccccgg gctgtccgtc gatgtacacc gcctgggttt cgtcccggta
    24121 gccctcgacc acggtcccca ggtccgagag ccggaccccg tacccgccct tgtcggccac
    24181 cacggtatcc gcgatctgtc ggaggctccg gtactccccg gtcgtgcgga ccaggtagtt
    24241 ccgggagccc tccgatatgg tgcctccgga gagctcgagg ttctggcccg ccagggtgga
    24301 ggcgactccg gtgagggtca ggttgtaggc gtccagccgg ttctgggaga tctcgacccg
    24361 gacgatcttc gagcgccctc ccgtcacctc cgcctgggct ataccgtcca cctgctcgaa
    24421 ccggggggcg atgtagtcct cggccagctc cttgagctcc tcggaactgc gcttgccgct
    24481 cacggcgatc cgcaggatag gcatggcgtt ggcatcgaag cggaaaatcc gggggcttcc
    24541 ggccccgtcg ggcagggagt tccgcacccg gtccagcttg tcccggatgt cgttcgtcgc
    24601 gtcctccaaa tccgtcccgt agttgaactc cagggtgacg cggctggacc cctcgctgga
    24661 actcgagctg atgctcttga gcccgttcac gttgatcagg gcggattcca ggacgctggt
    24721 cacccccttc tccacggact cggggccggc ccgggaatag gaggtggaga ccgagagccg
    24781 gggcatctcg ctttccggca tcaggtccag ggcgaggttc cccatggtat agaggcccac
    24841 gatcgccgtc agggcgaaga ctatggagat gaggacgggc cggtcgatga ccctgccggc
    24901 tacgctcacg gcctgtcccc ttcccgggtc ccgatgtccc ggactgcggc cccgtcggcc
    24961 agggcggcgg ccccctcgat caccacggtc tccccctcct ccaggcccga gacgacctcc
    25021 gccctcccgt ccacggccac tccccggacg acccggcgct tggaagccgt cccgtcgcgg
    25081 actacgaaga cgaaggatcc ctccgcgtcg gacctcagcg cggcctcccc cacgatcagc
    25141 cggtcgggat acgcgagggt gttcaggcgg atccgggcga acatgcccgg attgacccgg
    25201 gagtccggcc ggtcgaagcc cagccggatg gtcttggtgc gggaaacggg atccaccacg
    25261 ggggataccc ggaccacgga ggcggggaag gtaacgtccg ggtaggcttc cagggacacc
    25321 gaggcccgaa gccccgtccg gagcaccccg acgaaccttt ccggtacccg ggcctcgacc
    25381 tggacgtcct cgattagccc gatctcggcg accgcgatcc ccgtgcccac cagggagccg
    25441 accgcgacgg gcagggaggt cacggtgccc gagatcgggg ccaggacggg gttcagggag
    25501 aaggccgagc ccggggtaga ggggtccacc tcggccagga cctcgccctt ggaaaccctc
    25561 ccgcccaggg ggaccttgag ggacactagc cttccggcga tgtaggggta caccttcacc
    25621 gccgagtcgc tgaccacgtc cccgttggac tcgatgtaat cctggatcgt cccgcgccgg
    25681 acgggctccg tgcggaccga gaacgcggtg gtcgaggcgg accggcccgc tcgggcgccc
    25741 gagggaccgg ccgcggccgg ggaccctccc gcggccgcgg aggatcccgc cgctccgggg
    25801 ccgccggatc gggcgccgcc gccgggaccc tgtccggccg taccggcggc ggccgaggga
    25861 gccccgccgg acaccgcggc cccggccgcg ccggcggcct tgggcttggg caggagcagg
    25921 accagggcga ccgccgcgga aaaaaagacc agcaggacga tgttgcgtat cctcacgatc
    25981 acctccccag ggtcccgaag ggaagcccca gcgcgtattc caggtccaga agggcggaga
    26041 ccaggttgta cgcctccttg aggaggttcg cccgggcttc ctggaggttg tcccccgcct
    26101 tctgcaggct caggatgtcc ttggttccga aattgtacgc ctcctcggtc aggcgccagg
    26161 aacgctccgc catctccacc gtcaggcggc gggccgccag ggaggccagg gactgctcga
    26221 tggatctgcg cagggaccgt accgagatct ccgcccccgt cagggcttcc ttgagctggc
    26281 tttcggtctt gaccgtcgtg tcccgggcct tgtccgcggt ttcccgggcg gaggaccagg
    26341 gcatgaggtt gtccagggag aaggagagcg tggcgctcag ggaaccgttg tccgtccagg
    26401 gtccggaatt caccgattgg gggccgtagg aacccgagag gaccagggac ggggaccggg
    26461 tcgcggaccg ggccgcggcc tcctggaccc gggcgctctc cagggaggct ctcagggcgg
    26521 ccacggaggg ggcttccccc gaaagtccgg aaaaatcgag cgccgagacc gaggacgcct
    26581 cggccaggct gccgaccagg gagatcgggg caccctgctc aaggcccagg gtctgcttga
    26641 acctcgcctc ctgggactcg taggccgtca gggcgctctc cagggagggc ttcaggttct
    26701 ccaggttgac ccgggcggac agggcgtcca gctccgaggc caggcccgcc cttcgcttgg
    26761 cctccacctg atcgtacccc ttctgggcgg tggccaggga ctggcgcagg accgccaggt
    26821 tctcccgggc caggatcagg gaatagaagg atttgcggac ctccagctcc agggaacggg
    26881 ccgcggtctc gtaggccagg gactggacgt cccgggaggc ccgggccttc cgcacctcgt
    26941 gaagggtgcg gggcgccagg gtgacggaca cccccgcgga tccggacagg gttaccgcct
    27001 ccgtcgcgct ggaccgccgg gcgcccgcgg aaagggtgat ccccggggcc gccccgttcc
    27061 atgcccggga cagggcccgc tccgcggcgg ccaggtccac ccggctccgg gccagggcga
    27121 ggttgttggc cagggccaac tccacggcgg cttccggagt cagggccgcg gcgctctccg
    27181 gggccgcgga ggaccagggc tgggccgcct gcgcccgggc cgcccccacg gcgaccgcgg
    27241 cggccaggat caggatcgcg aatctctgca tgccgcaaga atacgcgatc caataggcct
    27301 tggactatat atcccgcatg aagggggcat aattttttgt tatatcggga gggtccgggc
    27361 cccttcccgt tgtccccgcc gggaacccgc gcctatactc gaagccgagg tgatgttcgg
    27421 catgaaggcc cgggttctga tcgtggagga cgtggcggag ctctccgacc tcgtcgccct
    27481 gtacctcgct aaggagggca tggaagcccg gcaggccgag accgcggagg cggccctggc
    27541 cctgatcccg gagtggaccc cggacctcat cgtcctggac ttgaacctcc ccgggatgga
    27601 cggattcgag ttcctccacg agttccgcaa aggcggacgc tgcccggtcc tgatcgtctc
    27661 cgcacgggac gcggacgagg acatcatagc cggcctgggc tacggggcgg acgagttcgt
    27721 caccaagccc ttctcccccc gggtcctgac cgcccgggtc cgggccctcc tccgccgggc
    27781 ccaggacgga accgcgtccc gggaaacccc gggcctcgtc cgcttcggtc ccttcaccct
    27841 ggacaccgag tcctgcctgc tcaggaagga ggggcggcgg gtccccctgt ccgccaagga
    27901 gtacgcggtc ctggccttcc tggccgccaa tccgggccgg cccctcgccc cccagtcgat
    27961 ctatgcctcg gtatggaagg gcgcctacgg ggacccgacc gccgtggcgg tctatatcca
    28021 gcgcctgcga aggaagctgg aggaggatcc cggggatccg aggttcctgg agacggtcca
    28081 cggcatggga taccggttca atccctcggg ggagccggca tgaggatacg gacccagttc
    28141 taccttctgg ccgcggggat cgtcatcata cccctggccg tcatgacggg cttctacttc
    28201 gcctggcgcc tgcgggaagc cccggcgccc cggatccccg ggtacgagga gatcctcaac
    28261 ctcgcggggg aacccgtgga ccgggcggcc tgggaggagt tggcgaggtt catggaacgc
    28321 aagcccccca cggtggagta tacggtcctg gatccctcca tgcgggtcct cttctccacc
    28381 atcgaggact tcggggcggg cgaggccgtc ccggacgcgg aggtgctgga ccgggttcgg
    28441 ggcaccggcg agaggtacct ctaccaactc gattcccccg tcaagacgga agggggacgc
    28501 ctcctggtcc tgacccggat cagccgggaa ggccggcgtc ctcccagtcc cttccagcgc
    28561 ctgttcacga ccttcgccac cctgctggcc gccctcttcg ccgtttccgc cgccggatcc
    28621 gtcctgatcg cccggtccct cacccagtcc gtcctggccc tggaggccgc cacccggcgc
    28681 atcgccgcgg gggagctgga cgccccggtc gccgtccggg gaggcgggga actggcttcc
    28741 ctcgcggcct ccctggtcag catgcgggac gccctgaagg aggaccggac ccgccggagc
    28801 cgcttcatca tgggggtgtc ccacgacctc aagacccccc tggccctgat caagggctac
    28861 gcggaggcca tcggggacgg gatggcggac gcccccggag agcgggaacg ctgtgtcgag
    28921 atcatcgggg ccaaggtgga ccagctggac gggatggtgg acgacctgat cgacttcgtc
    28981 cgcatggaca ccgccgagtg gaggcggcac ctccggtccg tggagctggc ccccttcctg
    29041 cgggacctgg tccgacgcac ccgggcggac gcggagctcc tgggccgccg ggtccaggac
    29101 gaggtggagc ttcccgaagg gatttcggcg cccatggacg aacgccttgc ccagcgggcc
    29161 ctggagaaca tcctgaacaa cgccctgcgc tacaccggtc caggaggcct cgtgcgcctg
    29221 gccgcccggg cggagccggg acgggccgtg gtgacgatct ccgacgacgg gccgggcata
    29281 cccggggaag acctgccccg gatcttcgac ctcttctacc ggggaaccgc ctcccgccgg
    29341 gagcagggcc tgggcctggg cctggccgtc gtgcggggag tcgcggattc ccacggctgg
    29401 gagatccggg tacggtccga gcccggccgg ggcagcgagt tcgagatttc gataccccta
    29461 tccgcacggc ccggctcagg gaccgctagg agattgtcgg gcttgcccat ctaaatagta
    29521 gaaaaatcgc atccatgcga tttttctact ttgcaaactc caaaggagcc ggataatcgg
    29581 gatttttggc gacttaggcg ccaaaaatcc acaagtccga caggctccta gctctgcagg
    29641 aagtcctccc gctggggggt gaacatgtcc accaccaccg agtcctccag gaacttcacg
    29701 ccgtgggaca cgttgggacc cgcgtagaag gtgtccccgg gacccagcac ttcctcccgg
    29761 ccgtccatgg taaggacgag cttcccggaa accacgtagc tggcctgctc gtgggggtgg
    29821 gcgtgggcat ccaccacgga gcccgccgcg aaccgcacct ccacgaccat gagcttgccc
    29881 ccccgggcca ggatcttgcg gctggcgccg gggaacaggt ccttcttctt cacgtccgcg
    29941 ttcctgatga ccatgatgcc tcctagaagt acagctcgta ttcttgggga accggggcgt
    30001 tgtacacgta ctccgcttcc ttgatcttgg attcgatcca gttggacagc agctcctccg
    30061 ggaaggcggg agccaggtag tcccggtcct tcttcaggcc cccgaggacg tggtacagcc
    30121 ccgtggggaa ggtggtcttg gggctcggct tggcggagga gaagccgagc cggacgggat
    30181 ccaggttccg gcggacgccg tccaggcccg ccaacaacat ggccgccagg aagtagtagg
    30241 tgttggccgt ggcgtccccg gtgcggaact ccacccgggc gtgtcccttc ttgaggtagc
    30301 cgggaatgcg gacggccgcg gcccgggagc cctgggcgaa ggtggcggat accggggcct
    30361 cgtagcccgg gaccaggcgg cggtaactgt tcgtgctggg attggagaag gccaggaggg
    30421 atcccgtccg ggcgtgggac aggatgcccg ccgtgtaggc cagcccggct tcggagaggc
    30481 cgtacagccc ctcacccggg aagatgctct tcccgccctt ggtgacgtac tggtgcacgt
    30541 gcataccgct gcccgcgatc ttgtacatcg gcttgggcat gaaggtgagc ttgagcccca
    30601 gctcgtccgc cgtggacttg aggatccact tggccaggac caccccgtcc gcggccttgg
    30661 agaggttcat gaagtccagc tcgatctcca tctgggaggc gcccacctcg tggtggtggt
    30721 acttgacggg gattcccgcc tccagcatgg cgcgcaccgc gcggtcccgg aggagaccgt
    30781 acttctcgtc cgggcccatg cggtggtagc ccttggacag gccgaagcgg tcccggtcct
    30841 cgtgctcctc ccccagcccc tcggcgctct ccacgaaata gtaggacttg tccacggtgg
    30901 tgtggtagcg gacgtcctcg aagacgtaga actccagctc caccagcatg tgggcttgct
    30961 cgccgatccc cgtggtccgg aggtactcct ccgccctgcg ggccaccacc cgagggtact
    31021 ggaggaaggg ccggccgtcc gtggagcgga cgtcgcagaa ggcgtgaagg acccggaact
    31081 ccccgcgctc ctccaggaag gccgaggcca tgtcgggtac cgcgatcatg tcgctggcgt
    31141 ggacctgggc gaaggagaag ttggaggcgt cgaagccgat accgtccgcc aggaccttgt
    31201 cggacacgta ggaccggggc agggagaccg agcgcagggc gccctggagg tcgacgacca
    31261 ggagggacag gtagtcgaag gaatccatgt cccccgtgta cggggcgatt ttcataggat
    31321 gctccttttg tcgcgctagg gggaggatat cctatcccgg cgggcggctt tgggcaagcg
    31381 agccccggcc gaggccggag gcgcggccgc tgtttcggcc tgcggaaggg ccgcgcggaa
    31441 ccggggcggc gatcccgctt tacaatcccg ctcgggccct gtatggtgaa agcgtcggcc
    31501 gtacggtcca atacccttaa aaacctcgca aggaggtccc catgaacccc gtcgagactt
    31561 ggcatgccga agccctgggc gcccaggccg tcaaggccct gcagaagaac ggcttcgacg
    31621 ccgtctacgt cgccaccgcc gcggaagccg cggacaaggt cctgtcctac atcaagcccg
    31681 gcgcctccgt cggattcggc ggctccatga ccgtcaaggc cctgggcatc caggagaagg
    31741 ccgcggccaa gggcgccgtc ctcctggacc acaacgcccc cgggctggcc ccggacaaga
    31801 agctggaaat cctgcgcagg cagctcacct gcgacgtctt cgtctcgggc tccaacgcgg
    31861 tcaccctgga aggggacatc gtcaacgtgg acgggaacgg caaccgcgtg gcggccctga
    31921 ccttcggtcc ctccaaggcc gtcgtggtcg tgggtgccaa caagatcgtc cgggacctgg
    31981 acgagggctt cgcccgggtg gagacctacg ccagccccat gaacaacaag cgcctggaca
    32041 agcccaaccc ctgcgtcaag accggaacct gcgaggactg ccagggcgag acccgcatct
    32101 gccgggtcta ccagatcctg cgccgcaagc ccagcctctc ggacttcacc gtcgtgatcg
    32161 tcggggaaaa cctggggttc tagaccctat ccggataaag gaacggggtc cccgcgggga
    32221 ccccgttcct ttatgtagtt cgacgaagcc cgcagggctt cgttgccgcg aggcgtaccc
    32281 gagtccgcct cgcgcggact ctgtgtctct gtggttagtt cgtggactcg gccgtggttt
    32341 cggccggctt gcccatcttt tcccgcaccg tcggaaggac cttccgggcc aggatgaggt
    32401 accggggcgg gagggaagcc ccctcggggg actcgtactt ggcgatgagg gcaaggaact
    32461 cctcctccgc gagggcgtac ttcccctgct tgtagtggat gaaggccgtc tcgtactcgc
    32521 aggtgaccag gaccgagggg tccgagccga aacgctcccg ggcggtctcg tagtagacga
    32581 tggcggcggc ccagtcggac cggtccgagg cctcctgggc gcgctggatc aactccatgg
    32641 ccgtggcgtc ctcggggatg acggggggct tggaggcgca ggacgcgatc agaaaaagag
    32701 cggtcaggac tgcgagaata gggaaaaagc gcttggtcat cgggattcct tcgaacggtc
    32761 aggccgtgta gatttcgtcc ttgtcgggca gggacttctt gagatcccgc atgtactccc
    32821 ggggatcccc catgtcctcg taggcgtcgc aggactcaat ggcgcgccgg gcgacggtga
    32881 agtatttgta gatcttctcc tcccccagct tcttcttcca tttgccggcg tcgcagcgga
    32941 cccggaggta gtaccgcccc gtcccgtggg cgggggtcgg cacgagcttg caatggacgc
    33001 agttggcgca gtagatgcgc gccgcggcgt ggtcggcggt ttcggattcc cgtagttccg
    33061 tgagggtttc catgtccctt gccccccttg cgggttcgat agtatataat cgggaatccg
    33121 cggtcaagcc ttgaccctta gccgtcccgc gtcggagccg ccatgatagc caccgtagtg
    33181 aacgccgtcg ccataatcct gggcaccctc gtggggctgg tcctgcgcaa gggattctcc
    33241 gaaaagatga aggacacggt cttcaccgcc gcgggctgca tcaccctggt cctggggatg
    33301 cagatggcct tcaagaccgg ccacgtcctg gccctggccc tggccctgtt catcgggggg
    33361 atctggggca cctggatgga cgtggagggg gcggtcttcc gcttcgggga ggtcttgaag
    33421 cgccgcttcg cgaagggcga ggagggatcc tccttcgcct acggcttcct gaacgcctcg
    33481 gtgctcttct gcgcgggggc catggccctg gtgggatcct tccgggcggg tgtggaggga
    33541 gactactcca tcctcctgac caagagtgtc ctggacggct tcatctccgt gctcttcgcc
    33601 ggggccatgg gggtgggcgt ggccttctcc gccctggcgg tcttcgcgta ccagggagcc
    33661 ctgaccctgc tctccgtccg catcgcccac ctggtcacgg aaaccatgct ctccgagctc
    33721 accgccgtgg gcggggtcct ggtcctgatg atcggtctca acctcctgga cctccgcaag
    33781 gtccggaccg gggacttcct gccggccctg ttggtgacgg tgatcttcgt cctgttgttc
    33841 ccttacgtgc cgttcctatg acgaagcggc aaccgatttc gcgttccaag gtcgaattcg
    33901 ggtgatgtcc accaacgtcg cgaacatacg gatggcccgg accctcttcc agtccgcctc
    33961 cgaccaatac tacatgctgg acaacgcggc catcatcatg cccgccatct cggacctggt
    34021 ggccaccagc ctcttccgga tcgccctggt cctggaccgc ccggtggacc ttcccctcct
    34081 ccaacgggcc ttggcgaacg tggccccccg gttcccctac ttcctggtgg agctccggcg
    34141 cgggttcttc tggtactact tccagccctc ccggaagcct ccggcggtca tcgcggacct
    34201 cggatccccc tgccagggct tcgatccccg caagacccgg gggcacctct tccgggtccg
    34261 ggcccggggc tcccgcatcg cctgcgagtt ctcccacatc ctggcggacg ggtacggggg
    34321 cctcatcctc gccaagaccc tcctggccga gtatttccgc ctccaggggg tggccagcga
    34381 gcccgctccg gacgtctttc cccccggctc cgagccgaac ccccacgaat gggaggacgc
    34441 ctaccaccgg tatttccggg agaactggcc ccatccggat ccccggcccc gggccttccg
    34501 ccctccgggt cccaccctcc cgctggggca gtaccgggtg ctgacgggga gcttcgatct
    34561 cgccgcggtc cgcgaggccg cccagtcccg ggacgccacg gtcacggaac tcctgaccgc
    34621 agccctgatg ttcgcctacc aggggatctg ggaatccctg ccccccaagg cgcggcggcg
    34681 cagggaatgc atggccgccg tggagatccc ggtgaacatg cgccgcttct atccctcccg
    34741 aaccctgcgg aacttcaccc tcttcatcct gcccgccgtg gatctccggc tcgggccgta
    34801 caccttcgag gagctcgtcc ggatcgtgca ccatcgggtg gccctggaga acgaggagag
    34861 ggagatagcc cggcagatct cccggaacgc cgggggcggg cgcaagctcc tggtccggct
    34921 ggtgcccctg gtcctcaaga acttcttcgc ccgcctgctc tacgactccc tgggggaggg
    34981 gctgatcacg ggactcctga ccaacctggg atccatgacc ctgcccgccc ccctggacga
    35041 gcgggtccga cgcttcgagt tcgtcccggc acccagccgc tccctgaaga ccaacgcggc
    35101 cgtctccagc tggaagggcg aactccacgt caccttcggc agccgcgccc ggtcccggga
    35161 ggtggagcgg aggttcttct ccgtgttgtc ttccataaac ctgcgggcga gaatagaatg
    35221 cgatgcggat ccgacggctc ccggagttcc ggagccccgg ccggcagaat cccgaacgag
    35281 ggaaggctca tggaagccga gagactgaac cagaagtacg ccaacgccgc gcgctccgac
    35341 gagttcggcc cctggtacgt cctggacaac gcggccatga tcttcccggt catgtccgac
    35401 cgcgtcgcca cctacctgtt ccgcctggcc gcgacgctca cggagcccgt ccacctccct
    35461 tccctctccg cggccctggc ccgcatcgcc ccccggtttc cgtactacat ggtggacctg
    35521 caccggggct tcttctggaa ctacctccaa cccctgaaca aaaccccctc gccggtctcc
    35581 gaccaacggt atccggtcca ggatttcgac gtgcgcaagc gcgggcagtt cccgttccgg
    35641 gtgcggacgc ggggccggag gatcgccctg gagatgcacc acgccctgtc cgacgggggc
    35701 ggggccttga tcttcttcaa gaccctcctg gcccagtatt tccggtaccg ggggatcgaa
    35761 tccgatccgg ccccggacat cttcgatccc gacgggacgc cccacccgga ggagtacgag
    35821 gacgcctaca accggttctt ccaatccgag cttcccgtac ccgaagcccg ccccaacgcc
    35881 ttccgcatcg tatcccccct gctcccgccc taccagttcc gggtgaccac ggggatcctg
    35941 ccggtggaca aggtcaagga ggtatccaag agcttcgggg tcacggtcac cgaactcttc
    36001 tgcgcctcca tcatggacgc cctccaggag atccgggctt cctaccccgc ctggatgcgg
    36061 cgccgcagcg gccccatggc caccctggaa gtgcccatca acatgcgcaa gatctacccc
    36121 acccggacca tgcggaattt cagcctctac ttcatgccct ccgtggacct ccgtctgggc
    36181 cgctacgact tcggggaact tgcggagctc gtgcattacc ggatcaagag cgagagcccg
    36241 gagcgggaaa taccccgcca gatggcccgg aacgtcggga tctcccgcaa gcggtacgtg
    36301 cgcctgcttc ccctggggat caaggaagtt gccatgcggg cggcctacaa cagcatggga
    36361 aagcggttca tgtccggggg agtctcgaac ctcgggctgc tgtccctgcc ggaaccgctg
    36421 ggctcccggg tggagcgaat ggaatgcctc atgcccccca gccgggtcac ctgcggcaac
    36481 ctgggagccg tgacctgggg ggacaggatc tacgccaact tcggcagcct catgaaatcc
    36541 cgggaggtcg agcgcctggc cttcaagcgc ctggtgtccc tgggcatccc cgtccgcctc
    36601 gagtgcaaca tggaggatcc gatatgaacc tgtgccccaa ctgcggagtc gacctgggag
    36661 acgaagcccg agcctgcccc ctctgcgggg tccgggtggg gcccgatgcg gacaagccag
    36721 gccccgtgat ccatcccctg ccccgcatgg agcccctctt cgatcccgac gggaaaggca
    36781 atttttctcc tcgggagaag ctcttcatca cccgggaggt catcaccgtg tgcctggcca
    36841 tcgcggcggg ctccgcggcg gccatcaact acctcgtctc cggccacctg acctggtccc
    36901 tctacccggt ggcttccatc gccttcgtct ggctctgcgt gggtttcccc ctgtacgtgc
    36961 ccaagcgccc cggactggca ctgaccgcgg ccgcactggg cctcgtcgga ttcctgggag
    37021 ccctggatct catcgacggc ccggagtaca cctggttcat ggccctggga ctccccctgg
    37081 ccctgtgcat cgaggtcttc gggatcggca ccgtcctggc ggtgatcaag gcccgtcgga
    37141 aggggctcaa catcgtatcc ctcatcctct tcggagtcac cgcgatctgc ctgtccatcg
    37201 agtacctgac cgaccggttc ctccgcggcg aggtccacct ccagtggtcc tccatggtgg
    37261 ccttcgccct ggtgcccgtg gggatcttcc tggtgtacat ccacctccgg atcgccacga
    37321 ccacctccct gcggaagttc ttccacgttt gagggttctg caatagaccg ggtctattga
    37381 cattttctct cgtttcttgt agtttattct cacatcgacg cagggtagag cagttggcag
    37441 ctcgtcgggc tcataacccg gaggtcgtag
//
LOCUS       DAOTEI010000012        36884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_764_length_36884_cov_6.340085, whole genome shotgun sequence.
ACCESSION   DAOTEI010000012 DAOTEI010000000
VERSION     DAOTEI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 36884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36884
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_36884_cov_6.340085"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="P5313_02045"
     CDS             complement(<1..196)
                     /locus_tag="P5313_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734376.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HRY79177.1"
                     /translation="MSRKGLINLRSLPSLVTLGLFLLMFGAGSLSFPGFFSLQNFLNL
                     FIDNAYLLVLAVGMTLVIVAG"
     gene            complement(193..1230)
                     /locus_tag="P5313_02050"
     CDS             complement(193..1230)
                     /locus_tag="P5313_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79178.1"
                     /translation="MAFLRNLSRKKIFWPLAALVLLMCFNLFFTPNFFRVEIKEGHLY
                     GNVIDILKNSAPIMFLAIGLTLVIATKGVDISVGSVVAISAGVVAMLIGGDLKGVPKN
                     PILLSMAAAVLVATLAGMWNGLLVSRVGMQPVIATLILLVAGRGIAMVTTNAMVVWLY
                     AKPFALLGQGYLLGLPFSLYMVAFLLLATMLVTRRTALGLFIEAVGINPTAARFAGIN
                     ARNIVFWVYAFSGFCAGISGLVVCSTVMSADGNNAGDGYEMNAILAVVLGGTSMSGGK
                     FYLMGSMVGALIVQTLTTTIYAFNVPPEIAFLVKAVVVWVVSLLQSPDLRSGMAGFLG
                     RRSEGGKEAAA"
     gene            complement(1232..2725)
                     /locus_tag="P5313_02055"
     CDS             complement(1232..2725)
                     /locus_tag="P5313_02055"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY79179.1"
                     /translation="MKKIRKEFPGVLALSGVDFRLWKGEVHALMGQNGAGKSTLVKVL
                     TGVHRHDGGEMLLEGRPIRPDSPLAAQKLGINAVYQEINLCPNLSVAENVFIGREPLR
                     LGRIDWKAMREKAREVLGRLNLSVDVTRTLSSCSIAVQQMVAIARVLDSAAKVLVLDE
                     PTSSLDEREVAQLFKVVRKLRDEGMAILFITHFLDQTYQIADRITILKNGEFVGEYEA
                     AKLPKLELVAKMLGKELSEFSQASPGEGEKSRTASVSAGELLRARGLGRRGGVAPFDL
                     DLDGGEVLGLAGLLGSGRTETARLLFGIDKADCGEISVKGKPAQIGSPRAAMALGIGF
                     CSEDRKNEGIFAELSIRENLILALQTKLGAFRFIAPKRQVEIADRLIRELGIKTPSSS
                     GEAKQLSGGNQQKVMLARWLATDPGILILDEPTRGIDVGAKLEIMEQILNLSRAGLGL
                     LFISSEFEEVLRCSGRVAVFKDKALVAQLAGPEMTESGIMRTIAGGR"
     gene            complement(2913..3875)
                     /locus_tag="P5313_02060"
     CDS             complement(2913..3875)
                     /locus_tag="P5313_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79180.1"
                     /translation="MKKVAVLMAVLALFAAAAFAAPKHWVVGFSQIGSESEWRTANTI
                     SVQNSFLEDTSFTLIYSDAQQKPENQIKALRSFIARKVDCIVFTALVETGYGPVLEEA
                     KRAGIPVIMIDRDVQASDRHLRLTIMGSDFVKEGEKAGTWLADYLKKKGLDDGKKQIN
                     IVELQGTTGSAPAIDRKTGFATVMKTHPNWVLTRSQTGNFTSSEGKQVMEAFLKADKN
                     IQVLYAHNDQMALGAIQAIKEAGLKPGKDIIIVGVDAVKGAFEAMVAGEMNCSVECSP
                     LLGPQAVQAVRDLRDGKKLPARIWTIEGIFDETNAAAALPTRQY"
     gene            complement(4036..4671)
                     /locus_tag="P5313_02065"
     CDS             complement(4036..4671)
                     /locus_tag="P5313_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79181.1"
                     /translation="MKNHSAGAFLALALFVAGILPASAADPAGLTCVGVYSETSAGSV
                     SWRTGSAAWTAVKVGDVLPASAEIRINVDRDWVEFVQNGKPGAVYEIPGPPSGEKILK
                     AADILKLKPRAVAFPKGAAGKPDAAYKDKLTVVEYLGRQIYLTADGDSRDIKYGDVLD
                     AKGKVKIIAINNTLTLMNAEGAVVKVIGPLTFTIADVLGNKNLYKFLNVQK"
     gene            complement(4895..6211)
                     /locus_tag="P5313_02070"
     CDS             complement(4895..6211)
                     /locus_tag="P5313_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY79182.1"
                     /translation="MGKRSDARLLPPLVADWLRDRAEDELTARSGSLPGIDVVFAQND
                     AMAYGAWRAFQAAGRTGIKLIGIDGLPGPMGGIDLVRKGILAATFTCPTGGKEAVAYA
                     VDLLSKKEGIPKKIYLRPRMVTAESLKEGTPASTTAPAARAPLGRPIVLGFSQVGSES
                     EWRVANTKSIKNAAAKAGIQLLFEDGRQRQENQIAAIRSFIERKVDIIAFSPVVESGW
                     DEVLREAKDAGIPVILSDRAVDVKDDTLWLSFMGSDFLEEGRRAARWLVEALKGEVSV
                     HIVELQGTIGSAPAIDRRIGFEEVIRDYPQFKIVASESGDFFKDQGYEVMKRILASGL
                     PRVDALFAHNDDMAIGAIQALEEAGLKPGTEVKIVSIDAAHGAFMAMLAGKLNCTVEC
                     NPLLGPQLMKAIQDYMAGTDLPIRMITSEGVYTAENARRDMKGREY"
     gene            complement(6309..6605)
                     /locus_tag="P5313_02075"
                     /pseudo
     CDS             complement(6309..6605)
                     /locus_tag="P5313_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(6752..7957)
                     /locus_tag="P5313_02080"
     CDS             complement(6752..7957)
                     /locus_tag="P5313_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79183.1"
                     /translation="MWKVLIADDEPKIRRGLRSLVSRCRDDLEVVAEAEDGEEAYARA
                     LEREPDILIIDVRMPLKDGLALIEELNAALPGRIVIIVSGHDEFEYAKAAVKLHVFEY
                     VLKPVDAEGFKAVIERAVAELEARRDSGKYAVWAREQLERNLPVLRERFLRDWITGTL
                     SRTEILEGLAFLGIELASPAALIAARFAERGPAGTAGGDRGRKLALVAMRTVLAEALP
                     PEGTYIFEDDTETVLALTPALREDELSAAVEAIERRAGEQVYQIPSISTRSIPDPVEG
                     ACDAYDEVRGELADGDGCEAFVVLARNYIDKHYWNPGLCLEDAAAELQISPGYLSRLM
                     KRETGFSFVEYLNRIRVRKATLLLSDPAAKAFEVAERVGYKSQHYFSRAYKKVTGSAP
                     TDRRKGAAP"
     gene            complement(7959..9776)
                     /locus_tag="P5313_02085"
     CDS             complement(7959..9776)
                     /locus_tag="P5313_02085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY79184.1"
                     /translation="MSKSKIAVRPPRRVPSGEARTLSLRFKLMVYFLVILALPLVTLG
                     IIGPSLYASSLERESMSYTEKMIGQVTMSIELRVLEMEKLIDLVSQMVPVAAFLEEGD
                     ASDAVRGEIRRIVSSVVGTHPEIAGLLVVTDRGAWEGEGFARTTRDPLTEERWYTEAR
                     TAPGRVSLAARPIGRNIRSTRRFGADEVVSIVKAVTGERDGRVRGAILVDMKLSYIEE
                     PFRYASLGKTDFLFIADARGEIVYAPVNEVVYRIPLAPLEDGAFSGLVRVGRGAYQVL
                     ARGSEYTGWRTVGVFSLAETLQEVSLVKYWTFLIGGMTMALAIVMAFFFMSAVTKPVL
                     RLRSLMKRVEQGDLAVRFAGAGGDEIAELGLGFDEMIERIQSLIEQVYREQQSKMEAE
                     LRILQEQIKPHFLYNTLDTIQWMAQEHRVDDVVCMVGALTSLFRIGLSKGREIISLPD
                     ELEHVRSYLCIQKMRYEDKFEYEVLEGEGLRGLRVMRLILQPLVENAVYHGIKERKGP
                     GSLAVETRIEHRPDGRGSDLILAVRDDGVGMDEARVARLNASLENGAVADLGGYGIRN
                     VHERIRLTFGKPYGLSFRSSPGRGTVVTIRHPILSSEDA"
     gene            9921..10550
                     /locus_tag="P5313_02090"
     CDS             9921..10550
                     /locus_tag="P5313_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY79185.1"
                     /translation="MDPSHVRLVWDRLKDRMLAFLRSKVSSPEDAEDLLQELFVRVHT
                     GLCCLEEWTSLERWIYRVARNLVIDHYRSRKRHEPLDSDPGIPSPYGAAEAEEDPEAR
                     IAFSLRETAEELPAPYREALTAVEYEGLSVADYARRSGISLSAAKSRVLRARERLKAS
                     LLECCHFELDRLGGIVNYEKRCAECDLRRAASASRPASGNPRPASPGAN"
     gene            10632..11723
                     /locus_tag="P5313_02095"
     CDS             10632..11723
                     /locus_tag="P5313_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRY79186.1"
                     /translation="MESIVDLFRHAVSQTLVSLSHNWPYLLAGILTAAALKAFLKSDR
                     VSAFLLRFRGAGVFAATAAAVTTPFCSCGTTAVVLGMMAGAMPLAPVVAFMVASPLSS
                     PGELVYSAGLFGWPFAIWFFASSILLGLGGGGVAAVLESRGWLKGQTRFTIAGAPSPV
                     PGGRAAARSPIRNPIPTSAAACACAAGPARPSDASAGPYRDASSACGCAAAATAPSPR
                     PGPLVSRTLEFLLAAAEVTRALVPMFLGFAFLGYLLNGLIPASWISALFGEGRAYGVP
                     LAATLGLPLYVNSEASLPLVRAFLDSGMTRGAVMAFLIAGSGTSVGAITGALTIARWR
                     VVGLVTAVLWIGAILAGWGFDLAAAAGWI"
     gene            complement(11792..12607)
                     /locus_tag="P5313_02100"
     CDS             complement(11792..12607)
                     /locus_tag="P5313_02100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY79187.1"
                     /translation="MESNGSTGPVVVLTGGTRGIGYGLAAAFLERGCSVVISGRDGAS
                     VAKSVRRLEAAAPGRAAGAACDVREFRDVQSLWDFAAGRFGRVDVWINNAGIGQPQSD
                     VDALDPDLVGNIFATNCAGALYGCKVALAGMKAAGRGAVYNMEGLGSGGEIVRGTTAY
                     GASKRALAYITDSAAREARGTGVIVGAIRPGMTATDLITAEYRGKPEEWVRVRRIFNI
                     LSDTVETVSPWIADRVLRNRRNGARIVWLTGAKAAGRFLTAPFVRRSIYPDLP"
     gene            complement(12636..12944)
                     /gene="hgcB"
                     /locus_tag="P5313_02105"
     CDS             complement(12636..12944)
                     /gene="hgcB"
                     /locus_tag="P5313_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HRY79188.1"
                     /translation="MLDEYLMNGSSLRLDPDACVGCEACVDVCPHGVFLVEEGKARIA
                     NRGACMECGACALNCPAAALTVRAGVGCAAAIILGKLRGTAPSCGDGGCSGDRQAGCC
                     "
     gene            complement(12954..14039)
                     /gene="hgcA"
                     /locus_tag="P5313_02110"
     CDS             complement(12954..14039)
                     /gene="hgcA"
                     /locus_tag="P5313_02110"
                     /inference="COORDINATES: protein motif:HMM:NF040863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HRY79189.1"
                     /translation="MIDLARRAAGPAERQAGFAALRELQVPWVERYLIREGRTVPVVR
                     TRLSLRDRRGTLKVRWGFGRGTYRVLPGLYAAGEPGPDSPVFVSANYKLSFDTLRSNL
                     AGIDGWILVLDTKGVNVWCAAGKGTFGTGELVSRIAKTRLGALVRHRELVLPQLGATG
                     VSAPEVARRAGWRVAWGPVRAEDIPAWLAAGRIKTEGMRTMTFTLRERLAVAPVELVH
                     SWPLTVAGLALAALYGLPADGSWASRTLPAAVLLGGLAPVGALAFPALLPGLPGRAFS
                     VKGAALGAAWGAACAALFGLPPSAAAAGVLVAAPVTAFLGMNFTGSSTFTCQTGALLE
                     VEKGFWPMAASLAAGLALGVLTRAFGA"
     gene            complement(14036..14518)
                     /locus_tag="P5313_02115"
     CDS             complement(14036..14518)
                     /locus_tag="P5313_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY79190.1"
                     /translation="MSVATIREFRRTLRTLEREVDLALQAQTGCCGVSSAQCHLLLTL
                     EESGPSSVGDLAAALELDSSTLSRTVDGLVRAGLAERREDPENRRRQIVSLSGAGREK
                     TDSINGLCDRYYRRLLGSLPPAEADVLHKALPILARALRSFRLAEGSAACCDRSGRPS
                     "
     gene            14798..15979
                     /locus_tag="P5313_02120"
     CDS             14798..15979
                     /locus_tag="P5313_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY79191.1"
                     /translation="MKPLSIGIVGTGFVAEIHAQTYAQIAGPSFRIAGCASGRPDNAR
                     AFSAKWSYPRAYNSFEELIRDPEVEAVDLCAPNDRHRDMITAAARAGKHVICEKPLTG
                     AFGEGSGAEAAGLLPKQGLFQEALANVRACGEAVRAAGVGFGYAENFVYAPPLAKARR
                     LLDRAGGTILDLRAEESHSGSHAEYARRWSRAGGGSLIRLGSHPIGAVLHLKRREGLR
                     RDGRPIRPVRVTAETGTHTKMPSFLGEAEHFLKAGWEDVEDWACALVTFEDGSHATIH
                     SSDGVLGGVRNSVSIYASNAVLQVNINPSDTLKVYAPRPGIFAGEYIQEKLETDGGWN
                     FPAPDEDWVRGYDAELTDFIDCFRSGREPESGLALAEDTVRVIYAAYLSAEEGIRVDL
                     P"
     gene            16025..17764
                     /locus_tag="P5313_02125"
     CDS             16025..17764
                     /locus_tag="P5313_02125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY79192.1"
                     /translation="MDPSARQGGFRNAVLFFFAAAILARPVSATPALVLPEEGKSVAA
                     RPYAECRVDAAGEEGYARVSRGAAFLSCERRSFRFDPAEGPSLWVRLSMELRPGAPPG
                     AAWYLEINNPALRTARLYVPVSGSTGGDVRILAAGWDPPSRGPVPFGRPGFRLPPDLD
                     PGRPVFLEVASSHEISFDLYVHSETAHSRREQALFLFLGTCLGGLGAMLLYNGFLYLA
                     LGDRHYGLFVLYLLCQLLYQATLAGIVRWLFPSVGVFLIRNVLIQFVLMNAGMVQFAR
                     AFLDTPRNAPRHDRAFRAALAGMALVALMSLAGRVREGNRLAYFLAQVQMPLLITASV
                     AALVRGFRPALLFLTSSSLLLAAAGVFILRNYGLLPNTLFTFHSVLAGTTAQSVLLSF
                     ALGQRIRILREEERRARERERQFRFASVTDELTGLFNRRHFNAALVQAVGEAEREGSG
                     LSLLMLDVDRFKDYNDEWGHPAGDRVLAELGAVIRASLRDCDAPCRYGGEEFAAILRG
                     ADLATARAIAERIRDRFARSAFEPRPGKTARVTVSIGAARLGGGESAEDFLERADRAL
                     YRAKDAGRNRVES"
     gene            17823..18374
                     /locus_tag="P5313_02130"
     CDS             17823..18374
                     /locus_tag="P5313_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRY79193.1"
                     /translation="MKNQAESDETESRRRLTEAYRAEKPRLLARLRAAGRTWEEAEDL
                     VQDVYAETLEGLPLVSGIRNLPAWINWLFTRRVTDLWRREKVRSASRVEVTEETLREI
                     IAGTGLDPLDEYVRDSLAEALNDALRALPAPQRKVLEAQVFGGLTFKEIAETTGENMD
                     TLMARKRYALANLARALRHWIEE"
     gene            18386..18760
                     /locus_tag="P5313_02135"
     CDS             18386..18760
                     /locus_tag="P5313_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79194.1"
                     /translation="MGERMKDGECRPDAWWEDRSVPQKVLIVIGFAILGAGFLVLIGW
                     VVMSLWNWLMPAIFGLPRVGYWQAWGLLILSSILFKGSGHGDKGGRADRKRRRELRKF
                     MKEGADGPDAEGGTAEPGGEAT"
     gene            complement(18844..20877)
                     /locus_tag="P5313_02140"
     CDS             complement(18844..20877)
                     /locus_tag="P5313_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY79195.1"
                     /translation="MRERFRVTSFLGTLFSVIVLSSLSIMGVSLERFVRADLTRHAEE
                     SNGLLAASVAHEISAFLDLHFMGLSLLRSRDFRPKGVDLLHEAYPAFDTVLFTDSSGR
                     VSRASMGSGDAGFDFSFREYFRIPFETGADFVSRPFVSGIRYTPSAVLAHPIPDGVAI
                     GYIDLGFLGDFLSSLPSSQNRSISVVDDRGAFVAHNSNLPLSGREDIAGLEPWFRERD
                     IQAGSGSTLVTRREGSEELLSWASTSGPSGWTVIVSEPSARVFASLLAFRISLVLVLV
                     AYAVLSLPLILGSVRLVRRDLAALARFSQDIAEGRTEAVLTFKGFRDFSLLASNLVRM
                     GRAIGERETRLRENERRLLETLDFMPVPVILLARDQRMELMNRAFSSVFGWAREDIDT
                     AEKWWSSVYPDPRYREEVRTFWQEYIDRLGRGERTEAPFRGRLRCKDGSCRSVVGQAA
                     LIAEGFVVTYVDMTESEQAAERTAASLREKEILLKEIHHRVKNNLQVVTSLLSLQASG
                     DPEQVRLFSDSINRIQVMAGIHELLYRSRDLAHIDLAEYGETIVHWLASTYSVGSSAP
                     ELELRMDRIELDIDKAVPCGLIMNEILTNTLKYAFPPDHPAPRIRVRAARDTEGFVVL
                     ELSDNGTGLPPDFDPERSSTLGFLLIRSLCQQIRGSWSLDRSGGTGWTIRFPV"
     gene            complement(20881..21984)
                     /locus_tag="P5313_02145"
     CDS             complement(20881..21984)
                     /locus_tag="P5313_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79196.1"
                     /translation="MMRRFAPARLAALLLAVLTVSCAPRGEITIGFVASLSGMDYMLG
                     TEGRDAAFLFVDRLNEEGGIGGRKLRLELRDLASDDSRAPDAVRDLKAAGAAAILGFF
                     SSSSALQALPVLEETGIPAVSPTSTSAELSGKDDPFFRTIMTSARDPAVLARRMRESR
                     HSRVLFLAAAYNRPYYETYRSGLVPEVALVDTVLYNRLEEIDYGRLEALARDPGFDAV
                     MIVASSLDSGTIAQNLVRQGIRKPLYLSGWAGNEDVITYGGKAVEGAVLVHQVDLDRA
                     STYPLSVLYKKTFGVSPGYGALETWDSLIFLTEGLKAARGDPGKLRSTLRGIRSFEGT
                     LGTIRMDEFGDAFRPLYLKRIIGGRFVVLGRAD"
     gene            22143..23213
                     /locus_tag="P5313_02150"
     CDS             22143..23213
                     /locus_tag="P5313_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRY79197.1"
                     /translation="MDDVLFRRVVRLADDFTVSADSRLRWMWGEALLGFALLELDRVL
                     GEDRYLPFLRAFGDHWAARDPRVDQSDTAAPGLVTYGLQKKLPGRGCERLTAKVLDYI
                     RNAPRVLDDSPNHLGSSPEGRLYPVSIWVDSLMMLSVFPAVYGAETGDRELLDFAARQ
                     PRLYAARLQDPEDGLWRHSWWVRAARPHPAGRVYWARGNGWVVASLPRILDRLPQDHP
                     ERPGILDILSRTSRALLPLQRPDGWWETVLLPPGKTYRESSATALIAAGWLHAAAAGY
                     LPSSYRAPAEMAFRSVVDSLAAGPDGRLAMPEISAPTIPLPVFPRLGYRLVPRGSNWT
                     YGIAALILAAVAGRKPGGAVAR"
     gene            23573..24562
                     /gene="dctP"
                     /locus_tag="P5313_02155"
     CDS             23573..24562
                     /gene="dctP"
                     /locus_tag="P5313_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY79198.1"
                     /translation="MRTWKKWVLLLLAAAVVPAVCFAQAKKYNLKLAHVAPVEEPYAV
                     AALKFAELVKAATDGNVNIEIFPNSVLGSSRDLVEGLQIGTVDATLCTAAVLANFVPK
                     SQVIELPFMFKSREHVYKVVDGPLADKIYEGAESKGLKIVSTWENGFRNITNNLRPIV
                     TPADMKGIKIRVMESQMYIDLFKALGANPTPMARGELFTALQQKTVDAQENPMGQIYT
                     SRFYEVQKYVSLTGHSYSPEVLVFSLATWKKLPKAYQTAIEKAAVEARDFNRKLSAEK
                     DDEFIRLCQEKGMQVTRLSPAQIAEFSKLMGPVWEKYYKVIGKDLIDKISSSK"
     gene            24637..25137
                     /locus_tag="P5313_02160"
     CDS             24637..25137
                     /locus_tag="P5313_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY79199.1"
                     /translation="MKAYKNLLDAVNRGTLWIAILAAAVMSVLVILQVVFRYAIQSSL
                     SFSEELARFLFVWTAFLGSAVALAKRAHVSIEIVVAHLPRKLKRGAILVTNGLGAIFF
                     LILIVYGILMTIRTLDQTSAAMELSMGFVYVVVPLSGLIMLMNLVYNGYEEFMNPDII
                     KSEGVE"
     gene            25140..26411
                     /locus_tag="P5313_02165"
     CDS             25140..26411
                     /locus_tag="P5313_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY79200.1"
                     /translation="MPLILFGSLAILLVLNVPIAVALGLATTFALLQSGLPLLVVVQR
                     MVTANDSFPLLAIPFFMLAGSIMTYGGVSKRLVAFADALVGWLTGGLGIVSNVSGMFF
                     SAISGSSAATTAAVGSVMFPEMEKRGYDRSFSAAIIAAAGETGIIIPPSVPMVVYGVI
                     AGVSIGDMFLGGFGPGILMGTTMAVLVYFVSRRHGYKGSSFGGLRKLGKSFLDALWGI
                     LMPVIILGGIYGGIFTPTEAAAIASLYGVLIGFFVYKDLKVSHFPKIMQSTIVSTAVV
                     MFIMNSAGLFGWLIAREQIPDAFARFFVSISSNPTVFLMLVNVLLLFTGMFLNASAAI
                     TILAPILVPVAIQLGIDPVFFGVIVVCNLAIGCLTPPVGVDLFVASTISGVSMARLSR
                     AIVPFLVVLIADLVVLTYVPAITMWLPNLMR"
     gene            26443..27330
                     /gene="ttdA"
                     /locus_tag="P5313_02170"
     CDS             26443..27330
                     /gene="ttdA"
                     /locus_tag="P5313_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HRY79201.1"
                     /translation="MDIERFTDIIARFLAVSGKILPDDIVKKLAELKAAEHMPLAKSV
                     YDSMSRNQDLAASLDRPSCQDTGVVNFFVSAGENFPGLGKIAGALREAVVRATAEAPL
                     RHNTVAIFDEKNDGTNVGERAPFVHWDIVPGSDKVKVETYMAGGGCSLPGRAITLMPS
                     AGYEGIVQYVFDTMVDWGINACPPLLIGIGIAGSVENASILSKRALLRPLGSHNPNPR
                     GAEMEGLLEDGLNRLGIGPQGLSGSSSVMGVHIESAARHPSTISVGINVGCWAHRRGA
                     IVLDKDLSYEILSHKGVRL"
     gene            27327..27935
                     /gene="ttdB"
                     /locus_tag="P5313_02175"
     CDS             27327..27935
                     /gene="ttdB"
                     /locus_tag="P5313_02175"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003259286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HRY79202.1"
                     /translation="MNKVLRTPISDSDLEDIRIGDVIYMDGILVTCRDVAHRRLIEFH
                     RELPVDLKGLAIFHAGPIVKKLPDGKYQMVSIGPTTSMRMERFEKEFVRQTGVKIIVG
                     KGGMAEETEEACRTYKALHCVFPGGCAVLAATEVEEIERVEWMDLGMPESLWVNRVKG
                     FGPLIVSIDAHGNNLFKKNKAVFNERKDRLVAEISEKVRFIQ"
     gene            28027..29490
                     /locus_tag="P5313_02180"
     CDS             28027..29490
                     /locus_tag="P5313_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HRY79203.1"
                     /translation="MKRVVCLALAALACVGFSFANGTREAEDGTAVLPLRDPVPTKSG
                     WVSGVVNGKPGKEVISYRGIPYAAPPVGNLRWKAPQPVTPWTGIRECTGLTKWPPQSF
                     PTVERYGSIPESFMSEDCLYLNVHVPVRTDGKPLPVMVWLHGGGLTNQSGSRDIYNMP
                     FLPAAGNVIQVSVVHRLGPIGYLAHPALSAESPQGVSGNYGNLDILAALKWVKDNIAA
                     FGGDPGNVTLFGQSGGGTKTAMVVASPLAKGLVDKAIIMSGRVDGTPLKEAEKIGLAL
                     AEKLGVTGTGPEALAALRALKWQDIIVTSLQEKYSADHTIDGWALPDAVSAIFKDGKQ
                     NDIPLMMGFTESDIPDYAIQGVSTVVSQMKMLKSPVYPYMFTYLPKNWRKDGAKAWHS
                     LDVGYIFGNPRKIAPVNFGDYGKRAGAVTPDAGFGPADDAMSEFMMQTWAQFARTGDP
                     SLPGGLKWPSYSRDKDQYVALDTVPEIRTGYSAFAAK"
     gene            29704..30375
                     /locus_tag="P5313_02185"
     CDS             29704..30375
                     /locus_tag="P5313_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY79204.1"
                     /translation="MKQISILPIRERVASEIRNAIFSGSFAPGEELGQDAIAAKLGVS
                     RMPVREALQILAGEGLVELRVNRGAIVKDVSTESIREHFELRLILECEAAARACGRIT
                     DFSELDYIHEEQRKAIENQDVAKANMCNQALHMYIWEHSGNKKLKNILVQLWNGLSIG
                     TVVRPIDHMRLSFGEHGEIIQAMKSRDENRARAVMAAHLKRSMENMIQRPTAEKATES
                     PSQTA"
     gene            complement(30502..30990)
                     /locus_tag="P5313_02190"
     CDS             complement(30502..30990)
                     /locus_tag="P5313_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY79205.1"
                     /translation="MIETILTRRSVRSFLPDPVPPDSLEALLKAGFHAPSAMNQRPWH
                     FVVLEGERLEGLLRGLANTPKGAPLALVVCVDENLERLTRAGDSDAAAASQNILLAAH
                     SLGLGAVWSAVLPGSEGFVRGYLGLPEKIRPFSVIPVGKPAQKPAPVDRYDGSRVHRN
                     GW"
     gene            complement(31091..32704)
                     /locus_tag="P5313_02195"
     CDS             complement(31091..32704)
                     /locus_tag="P5313_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HRY79206.1"
                     /translation="MTAPRLALGIDSSTQGTAAVLIDLESPALAAEARVAYRGDPRLA
                     GFGLKGNEFLLPPREEGEADQPVGLFLAALDAVLSDLPRDLLSRVAAVAVSAQQHGQV
                     WLNPRAEGAFRALRLAGAGKPGSPGLAEHLASALASGRAPIWMSSNAAREAGELRTTA
                     GGADAMTALSGSDSPVRFTGAVLRRRALREPRTYAACSRIHLISSFLASVLAGRPDAP
                     ADWGNGAGTSLMDWAARRWSPVLLEAAAAGLPGGAEGLHARLPALAHPLHVVGTAARY
                     FTERFGIPDDAVVAAGSGDNPQTKVLSPGGLLSLGTSFVLMSEGGRPHPSANAMYDGL
                     GRPFLFGCRTNGSLSWESVRISHGLAGDDFSASERALASTPPGSVLRILQGEPESFPE
                     SPPLNLGGRKDFAEDYSGAVDSALGLLWLGSRPFSGDPAGPAAGIAAAGGGAGSVEVL
                     RRVAAIWDRPVRRIGRAGAAAGAAVAAASARISSEEREEFAARVAAVVADRGPPILPD
                     PAYRAAYHGEEGYLRHLARFFESASGRSL"
     gene            complement(32730..34052)
                     /gene="xylA"
                     /locus_tag="P5313_02200"
     CDS             complement(32730..34052)
                     /gene="xylA"
                     /locus_tag="P5313_02200"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HRY79207.1"
                     /translation="MNDLFPSIPKIPYEGPDSDRELAFKHYDPAARTPDSPEPGTLKE
                     CLRFSMAFWHSMTGTGKDPFGDGTLLRPWDGVDDEMEVARLRMRSVFELTEKLSIPFF
                     CFHDRDIAPEGDTLRETNRKLDEITALTKDLMRTSPTRVLWGTANLFNHPRFLHGAAT
                     SPDPRVFAYAAAQVKKALEVTLELGGQNYVFWGGREGYETLLNTDMKREQDHLARFLH
                     MAADYAREIGFDGQLLIEPKPKEPTKHQYDSDAAACWAFLQEYGLSSRFKLNIETNHA
                     TLAGHTMQHELRYARERGILGSIDANQGDELLGWDTDQFPTDLYMATLAMYEVRKNGG
                     LHSGGLNFDAKVRRGSHRPEDLAIAHIAGMDTMARGYKAAGRMIADGALDRPLAERYA
                     LWGKGVGAAVEAGRESFGSLESFALDIEDFDLPSGGQEFLESRVNRYL"
     gene            34190..35008
                     /locus_tag="P5313_02205"
     CDS             34190..35008
                     /locus_tag="P5313_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRY79208.1"
                     /translation="MRFEDIVFVYHLRDERQIAWHGRRHAHREGEYELHYFISGEGSF
                     RNGSGTWTIRPGSLHMTPPGSVHQILATNLRKPITYFAVLFSASGDQELFGLLDRAVD
                     AMARPVDIGTSHRFFFADLLERSSSGHADLVEASRHSFLSFLYGLVAGAPSSYGAPEN
                     SHVEKAIAIMQASIEDNLDLDSLSGRLGVSREHLVRVFAQRMRMPPMRYYSRLKIEAA
                     RAMLSSTNLGIGTISEKLGYENQFGFSRAFKNVVGFSPTEYRARCLQKADFAHS"
     gene            35121..36173
                     /gene="chvE"
                     /locus_tag="P5313_02210"
     CDS             35121..36173
                     /gene="chvE"
                     /locus_tag="P5313_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY79209.1"
                     /translation="MKKGILILLALAVVCSSAFAQGMLIGVAMPTQSLQRWNQDGANM
                     KAQLEAKGYRVDLQYANNDINTQIQQLENMITKGCKVLVIASIDGSVLSDVLKKAQAG
                     GIKVIAYDRLIMNTPNVDYYATFDNFKVGVIQGKYIESALGLDKGKGPFNIELFAGSP
                     DDNNATFFFNGAMSILRPYLDSKKLVIPTGQKDFTVIAIQSWESAKAQARMDNLIAAK
                     YAGSTKLDAVLSPNDSLAIGIVASLKNVGYGTARKPYPILTGQDCDKANVKAMIAGQQ
                     SMSIFKDTRTLAAKVVEMVEAIMQGKTVPVNDTKTYNNGVKVVPSFLCDPVYADKANY
                     KALLVDSGYYTEADLK"
     gene            36266..>36884
                     /locus_tag="P5313_02215"
     CDS             36266..>36884
                     /locus_tag="P5313_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY79210.1"
                     /translation="MVGNILEMRSITKLFPGVRALSDVNLSVRRGEIHALVGENGAGK
                     STLMNVLGGVYPHGTYSGQLVFEGEECSFRTIHDSERKGIVIIHQELALVPYLSVGEN
                     IFLRNERSRGGVIDWDKTHRDAAKLLELVGLKEGTRTAVKDLGVGKQQLVEIAKALSK
                     NVKLLVLDEPTASLNEDDSEHLLGLLLDLKAKGVTSILISHKLGEV"
BASE COUNT         6065 a        12399 c        12308 g         6112 t
ORIGIN      
        1 cccccgccac gatgaccagg gtcatgccca cggccagcac cagaaggtag gcgttgtcga
       61 tgaagaggtt caggaaattc tggagggaga agaagcccgg gaaggacagg gacccggccc
      121 cgaacatgag gaggaacagc cccagggtca ccagggaagg caggctcctc aggttgatca
      181 aacctttccg gctcatgccg ccgcctcctt tccgccctcg gatctccggc ccaggaaacc
      241 ggccatcccg gatctgagat cgggggactg gaggaggctc acgacccaca cgaccacggc
      301 cttcaccagg aaggcgatct cgggggggac gttgaaggcg tagatcgtcg tggtcagggt
      361 ctggacgatg agggcgccga ccatggagcc catgaggtag aacttcccgc cgctcatgga
      421 ggttcccccg aggaccaccg ccaggatggc gttcatctcg tacccgtccc ccgcgttatt
      481 gccgtccgcg ctcatcaccg tggagcagac gaccagcccc gatatcccgg cgcagaagcc
      541 cgagaatgcg tagacccaga agacgatgtt ccgggcgttg atgccggcga aacgcgcggc
      601 ggtcgggttg atgccgacgg cctcgatgaa caggcccagg gcggtccttc gtgttaccag
      661 catggtcgcc aggaggagga aggccaccat gtacagtgag aagggcaggc ccaggaggta
      721 gccctgcccg agtagggcaa agggcttggc gtagagccag acgaccatgg cgttggtggt
      781 caccatcgcg atcccccgtc ccgcgaccag gaggatgagg gtcgcgatca cgggctgcat
      841 tcccacccgg gacactaaaa gaccgttcca catccccgcc agggtggcga ccaggaccgc
      901 cgcggccatg gacaggagga tggggttctt cgggaccccc ttcaggtccc cgccgatcag
      961 catggccacg actcccgcgg agatcgccac cacggacccg acggagatgt cgaccccctt
     1021 ggtcgcgatg accagggtca ggccgatggc taggaacatg atgggcgcgg agttcttgag
     1081 gatgtcgatc acgttgccgt agaggtgccc ctccttgatc tcgacccgga agaagttggg
     1141 ggtgaagaag aggttgaagc acatgaggag cacgagggcg gccaggggcc agaagatctt
     1201 cttcctcgag aggttccgta ggaatgccat gctatctgcc tcccgctatg gtgcgcatga
     1261 tgcccgattc ggtcatctcc ggcccggcca gctgggccac cagggccttg tccttgaaga
     1321 ccgccaccct cccggagcag cggaggacct cctcgaactc ggaggatatg aacaggaggc
     1381 ccagcccggc gcggctgagg ttcaggatct gctccatgat ctccagcttg gcgcccacgt
     1441 cgatgccccg ggtcggctcg tccaggatca ggatgcccgg gtccgtggct agccagcggg
     1501 ccagcatcac cttctgctgg ttcccgccgg acagctgttt ggcctccccc gaggaggacg
     1561 gggtcttgat cccgagctcc cggatcagtc ggtcggcgat ctcgacctgc ctcttggggg
     1621 cgatgaaccg gaaggctccg agcttggtct gcagggccag gatgaggttc tcccggatcg
     1681 agagctccgc gaagatcccc tcgttcttgc ggtcctcgga gcagaacccg atgcccaggg
     1741 ccatcgcggc ccggggggat ccgatctggg ccggctttcc cttcacggag atctcgccgc
     1801 agtcggcctt gtcgatcccg aagaggagcc gggcggtctc ggtccttccc gatccgagga
     1861 gccccgccag gcccaggacc tccccgccgt ccaggtccag gtcgaagggg gctacgcccc
     1921 cgcgacgccc aagtccccgg gcgcgcagaa gctcccccgc cgaaacggag gcggtccggg
     1981 atttctcgcc ttcccccggg gaggcctggc tgaactcgga gagctccttg cccagcatct
     2041 tggccaccag ctcgagcttg gggagcttgg cggcctcgta ctccccgacg aactcgccgt
     2101 tcttgaggat cgtgatccgg tcggcgatct ggtaggtttg gtccaggaag tgggtgatga
     2161 agaggatggc catgccctcg tcccggagct tccggacgac cttgaacaac tgggcgacct
     2221 cgcgctcgtc caggctcgag gtcggttcgt ccaggaccag gacctttgca gcggagtcca
     2281 ggacccgggc tatggccacc atctgctgca cggcgatcga gcacgatgac agggtccggg
     2341 tgacgtccac ggacaggttg agccgtccga ggacctcccg cgccttttcc cgcatggcct
     2401 tccagtcgat ccggcccagt cgcaggggct cccggccgat gaagacgttc tcggccaccg
     2461 agaggttcgg gcagaggttg atctcctggt acaccgcgtt gatcccgagc ttttgggcgg
     2521 ccagggggga gtcgggccgg atcggtcggc cttccaggag catctccccc ccgtcgtggc
     2581 ggtgcacccc ggtcaggacc ttcacgaggg tggacttgcc cgcgccgttc tgtcccatca
     2641 gggcgtgcac ctcccccttc cacaagcgga aatccacgcc ggacagggcc aggacgcccg
     2701 ggaattcctt tctgatcttc ttcatctcca ggatcggagc ttgttcggac atcggcttcc
     2761 tccagcgggg ttgggcggcc gggacgtccg gtattcggag cgcccggcgc ttcggaagga
     2821 acggggatcc ttccgggcat gccggaggcg cggtacggga cccgcccgcc cgcgcctccg
     2881 aagcccgcgt ttttacattc tttcggccgg gctcagtact gccgggtggg cagggccgcc
     2941 gcggcgttcg tctcgtcgaa gatgccctcg atcgtccaga tccgggcggg gagcttcttg
     3001 ccgtcccgga ggtcccggac ggcctgcacc gcctgggggc cgagcagggg gctgcactcc
     3061 acggagcagt tcatctcgcc cgcgaccatg gcctcgaagg cgcccttcac cgcgtccacg
     3121 ccgacgatga tgatgtcctt gccgggcttg agtcccgctt ccttgatggc ctggatggca
     3181 cccagggcca tctggtcgtt gtgggcgtag aggacctgga tgttcttgtc cgccttcagg
     3241 aaggcctcca tgacctgctt gccctcggag ctggtgaagt ttccggtctg ggagcgggtg
     3301 agcacccagt tcggatgggt cttcatgacc gtggcgaatc cggtcttgcg gtcgatggcc
     3361 ggagcggacc cggtggtgcc ctggagttcg acgatgttga tctgcttctt gccgtcgtcc
     3421 aggcccttct tcttgaggta gtcggcgagc caggtcccgg ctttctcgcc ttccttcacg
     3481 aagtcggaac ccatgatggt aagccggagg tggcggtcgg aggcctggac gtcccggtcg
     3541 atcatgatga cggggatccc ggccctcttg gcctcctcga ggacggggcc gtatccggtc
     3601 tcgaccaggg ccgtgaagac gatgcagtcg accttgcggg cgatgaagga gcgcagggcc
     3661 ttgatctggt tttcgggctt ctgctgggcg tccgagtaga tcagggtgaa gctcgtgtcc
     3721 tcgaggaacg agttctgcac ggagatggtg ttggccgtgc gccattcgct ctcggagccg
     3781 atctgggaaa atccgacgac ccagtgcttg ggcgccgcga acgcggccgc ggcgaacagc
     3841 gccagtacgg ccatcaacac tgccactttc ttcatgtcgt attcctcctt ggagatctat
     3901 cgtcgccggc cggcgacgcc gtccccgtcc gccttggcgg tccgggaaat cgcccgcggc
     3961 cccggcctgc gccggaaacc ccgcgcggac aatccgatcc ttgcgttggc gccttccacc
     4021 tccttccggg ttcgtctact tctggacgtt gaggaacttg tagaggttct tgttgccgag
     4081 cacgtccgcg atcgtgaagg tgagcggccc gatgaccttc acgacggcgc cctccgcgtt
     4141 catgagtgtg agggtgttgt tgatcgcgat gatcttgacc ttgcccttgg cgtccaggac
     4201 gtcgccgtac ttgatgtccc gggaatcccc gtccgcggtc aggtagatct gccgtcccag
     4261 gtactcgacc acggtcagct tgtccttgta cgccgcgtcg ggcttgcccg cggcgccctt
     4321 cgggaaggcc accgcccggg gcttcagctt gaggatgtcc gcggccttca ggatcttctc
     4381 ccccgacggc gggccgggga tctcgtagac ggcgccgggc ttcccgttct gcacgaactc
     4441 cacccagtcc cggtccacgt tgatccggat ctccgcggac gcgggcagga cgtcgcccac
     4501 cttgaccgcg gtccacgcgg cggatcccgt gcgccagctc acggagcccg cgctggtctc
     4561 cgaatagacc ccgacgcagg tgaggccggc gggatccgcg gcggacgccg gcaggatccc
     4621 ggcgacaaaa agggcgagtg ctaggaacgc tcctgcgctg tggttcttca tgacgggctc
     4681 ctccgcggac gaaagatggc aggcggcacg cgccgtacgg ggatagtatg ggggtggttt
     4741 tcaaaaacgc gatttagcgg aaacggcttt tttttgcact attcggcgat cggggcgggc
     4801 gcgccgagcg gatgtcgtcc gctcgttccg agcgcccggg aatggtgtcc gggaccgacg
     4861 cccggccacg gcgctcgtcc gtacggccgg accgtcagta ctcccggccc ttcatgtccc
     4921 ggcgggcgtt ctcggcggtg tagaccccct cggaggtgat catgcggatg ggaaggtccg
     4981 tcccggccat gtagtcctgg atggccttca tgagctgggg gccgaggagg ggattgcatt
     5041 ccacggtgca gttgagcttc cccgccagca tggccatgaa ggccccgtgg gcggcgtcga
     5101 tggagacgat cttcacctcc gtgccgggct tcagccccgc ctcctccagg gcctggatgg
     5161 ccccgatcgc catgtcgtcg ttgtgcgcga agagggcgtc gacgcggggg agcccggagg
     5221 ccaggatccg cttcatcacc tcgtagccct ggtccttgaa gaaatccccg ctctcggagg
     5281 cgacgatctt gaactgcggg tagtcccgga tcacctcctc gaacccgatg cggcggtcga
     5341 tggcgggggc cgagccgatc gtgccctgga gctccacgat gtgcacgctg acctccccct
     5401 tcagggcctc cacgagccac cgggccgcgc gccgtccctc ctccaggaag tcggatccca
     5461 tgaaggagag ccacagggtg tcgtccttca cgtccaccgc ccggtccgac aggatcacgg
     5521 ggatgcccgc gtccttggcc tcccggagca cctcgtccca gccggactcc accaccgggg
     5581 agaaggcgat gatgtccacc ttccgctcga tgaaggaccg gatcgcggcg atctggttct
     5641 cctggcgctg tcggccgtcc tcgaagagca gctggatgcc ggccttggcc gcggcgttct
     5701 tgatggactt ggtgttggcc acccgccact cgctctcgga cccgacctgg gagaagccca
     5761 ggacgatggg ccttcccagg ggagcccggg ccgcgggagc ggtcgtcgag gccggggtcc
     5821 cttccttcag ggattcggcg gtgaccatgc ggggccggag gtagatcttc ttggggatgc
     5881 cctccttctt cgagagcagg tcgacggcat aggccaccgc ctccttgccg cccgtggggc
     5941 aggtgaaggt ggcggccagg atgcccttgc ggaccaggtc gatcccgccc atgggccccg
     6001 gcagtccgtc gatgccgatg agcttgatcc ccgtacgacc ggcggcctgg aaggcccgcc
     6061 aggccccgta ggccatggcg tcgttctggg cgaacacgac gtcgatcccc gggagggatc
     6121 cgctccgggc tgtaagctcg tcctccgccc tgtcccggag ccagtccgcc accaggggcg
     6181 gaaggagccg ggcgtcgctc cgcttcccga tctcctcccg gaagccctcg ctccgctcga
     6241 ccgcgggggg gggagcccga gcggccctcg atgaccagga cgtccccgcc cccgggcccc
     6301 aggagcccgg aggcgaccct tccggcctcc cgccccaggc gctcgttgtc cgggccgatg
     6361 aacagggtgt agtcgtagcc ctccaccgcc cggtccagga cgatgacggg gatgcgcgag
     6421 taggtctccc gcacgaccgg ggtcagggcc tgggagtccg tgggggacac gatcaaaagg
     6481 tcgatgccga agcccaggag gcgccggacg tcctcgatct gccggacgct ggaatccgcg
     6541 gcgtccgcgt agatgacccg caggcccttc tcccgggccg cggcctcccg gatctcctcg
     6601 ttcatggcga tgcgccaggg ctccgtcagg ttggcctggg acaccccgat gaggaagcgc
     6661 accccgctct cgctggcggg gggacgcccc ccgcaggcgg ctgccgccag ggccagggcg
     6721 caggccagga cccgaaggac agaccgcggt ttcacggggc cgcccccttg cgccggtcgg
     6781 tgggggccga accggtcacc ttcttgtagg cgcggctgaa gtagtgctgg ctcttgtaac
     6841 cgacccgttc cgcgacctcg aaggccttgg ccgcgggatc gctcaggagc agggtcgcct
     6901 tccggacccg gatgcggttc aggtactcca cgaaggagaa ccccgtctcc cgcttcatga
     6961 ggcgggagag gtatccgggg ctgatctgga gctccgcggc cgcgtcctcc aggcagagcc
     7021 ccgggttcca gtagtgcttg tcgatgtagt ttcgggcgag gaccacgaag gcttcgcagc
     7081 cgtcgccgtc cgccagctcc cctcgcacct cgtcgtaggc gtcgcaggcg ccctccacgg
     7141 gatccgggat cgagcgcgtc gagatggacg ggatttggta gacctgctcc ccggccctgc
     7201 gctcgatggc ctccacggcc gcggacagct cgtcctcccg gagggcgggg gtcagggcca
     7261 ggacggtctc cgtgtcgtcc tcgaagatgt aggtgccctc ggggggcagg gcttccgcga
     7321 gcaccgtccg catggccacc agggcgagct tccggccgcg gtcgcccccc gcggtccccg
     7381 cggggccgcg ctcggcgaac cgggcggcga tcaacgcggc gggggaggcc agctcgatgc
     7441 ccaggaacgc caggccctcc aggatctcgg tgcgcgagag ggtcccggtg atccagtccc
     7501 gcaggaaacg ctcccggagc accggcaggt tgcgctccag ctgttcccgg gcccagacgg
     7561 cgtacttgcc gctgtcccgc cgggcctcca gttcggccac ggcgcgctcg atcacggcct
     7621 tgaagccctc cgcgtccacg ggcttcagga cgtactcgaa tacgtggagc ttcaccgccg
     7681 ccttggcgta ctcgaactcg tcgtgccccg agacgatgat gacgatcctg cccggcaggg
     7741 cggcgttgag ctcctcgatc agggcgaggc cgtccttgag gggcatgcgc acgtcgatga
     7801 tgaggatgtc cggctcccgc tccagggccc gggcgtaggc ctcctccccg tcctccgcct
     7861 ccgccaccac ctccaggtcg tcccggcagc gggagaccag ggagcggagc ccccggcgga
     7921 tcttgggctc gtcgtcggcg atcagcactt tccacatgtc aggcatcctc cgaactcagg
     7981 atcgggtgcc ggatcgtgac cacggtgccc cggccggggg agctccggaa ggagagcccg
     8041 tagggcttgc cgaaggtcag ccgtatccgc tcgtggacgt tgcggatccc gtagcccccg
     8101 aggtccgcga ccgccccgtt ctccagggag gcgttgagcc gggcgacccg ggcctcgtcc
     8161 atcccgaccc cgtcgtcccg gaccgcgagg atcaggtcgg agccccggcc gtccggcctg
     8221 tgctcgatgc gggtctccac ggccagggaa cccgggccct tcctctcctt gatcccgtgg
     8281 tagaccgcgt tctccaccag gggctggagg atgagccgca tgacccggag gccccggagg
     8341 ccctccccct cgaggacctc gtactcgaac ttgtcctcgt aacgcatctt ctggatgcag
     8401 aggtagctcc gcacgtgctc gagctcgtcc ggcagggata tgatctcccg ccccttggac
     8461 aggccgatcc ggaacaggct ggtcagggcc ccgaccatgc agaccacgtc gtccacccgg
     8521 tgctcctggg ccatccactg gatcgtgtcc agggtgttgt acaggaaatg gggcttgatc
     8581 tgctcctgca ggatgcgcag ctccgcctcc atcttcgact gctgttcccg gtagacctgc
     8641 tcgatgaggc tctggatccg ctcgatcatc tcgtcgaagc ccagccccag ctccgcgatc
     8701 tcgtccccgc cggctcccgc gaagcgcacg gccaggtccc cctgctccac gcgcttcatg
     8761 aggctgcgca ggcgcaggac cggcttggtt accgcggaca tgaagaagaa ggccatgacg
     8821 atggccaggg ccatggtcat ccccccgatc aggaaggtcc agtacttgac caggctgacc
     8881 tcctggaggg tctccgccag ggagaacacc cccacggtcc tccagcccgt gtattccgat
     8941 ccccgggcca ggacctggta ggccccgcgg ccgacccgca ccaggccgga aaacgccccg
     9001 tcctccaggg gggccagggg gatacggtac accacctcgt tgaccggggc gtacacgatc
     9061 tcgccgcggg cgtcggctat gaacaggaag tccgtcttgc ccagggatgc gtaccggaag
     9121 ggctcctcga tgtaggatag cttcatgtcc accaggatcg cgccccggac cctcccgtcc
     9181 cgctccccgg tcacggcctt cacgatggag acgacttcgt cggccccgaa acgccgggtg
     9241 ctccggatgt tacgcccgat cggccgggcc gccaggctga cccgtcccgg ggccgtccgg
     9301 gcttccgtat accatcgctc ctcggtgagg ggatcccggg tggtgcgggc gaagccctcg
     9361 ccttcccagg cgccccggtc cgtgacgacc aggagtcccg cgatctccgg atgcgtcccg
     9421 accacggagg agacgatcct acggatctcc ccgcgcaccg catcggaggc gtccccctcc
     9481 tccaggaagg ccgcgacggg caccatctgg gagacaaggt cgatgagctt ctccatctcc
     9541 aggaccctaa gctcgatgct catggtgacc tgcccgatca tcttctcggt ataggacatc
     9601 gactcgcgtt ccagggagct cgcgtagagg gagggaccga tgatgccgag ggtgaccagg
     9661 ggcagggcga ggatgacgag gaaatacacc atcagcttga accggaggct gagggtccgg
     9721 gcctctcccg agggaacgcg gcggggcggc ctcactgcga tcttggattt cgacatccct
     9781 ttccccgtgt acgtacacga ttgtacatcg ggaatcggga tttgtggcgg ggaatcggag
     9841 gcggcttgcc gtccccggta cggaatcgta gactgggtgc cgcggcgggt ccgccgcgaa
     9901 tccgcgaagg agaagtcggc atggatccct cccacgtccg tcttgtatgg gaccggctca
     9961 aggacaggat gctcgcgttc ctccgatcca aggtttcctc ccccgaggac gcggaggacc
    10021 tgctccagga gctcttcgtg cgggtgcata cggggctctg ctgtctggaa gagtggactt
    10081 ccctggaacg ctggatctac cgggtggcac gcaacctggt catcgaccac taccggtccc
    10141 ggaagcggca cgagcctctc gattccgatc ccgggatccc ctctccctac ggggccgcgg
    10201 aggcggagga ggatccggag gcccggatcg ccttctccct gcgggagacc gcggaggagc
    10261 tccccgcgcc gtaccgggaa gccctgacgg cggtcgagta cgagggcttg tcggtcgccg
    10321 actacgcccg ccggtccgga atatccctgt ccgccgccaa atcccgggtc ctgcgcgccc
    10381 gggaacggct caaggcctcc ctcctggagt gctgccactt cgaactggac cgcctgggag
    10441 ggatcgtgaa ctacgagaag cgctgcgccg agtgcgacct gcgccgcgcg gcctcggcgt
    10501 cccggcccgc gtccgggaac ccccgccccg cgtctcccgg ggcgaactag gccccgcccc
    10561 gaacgatttt ccgcatcctc cggcacgccc gatcgtcagt ccggtcggaa ccgaaatcac
    10621 cggaggtacc tatggaatcg atcgtcgacc tgttccgcca cgcggtctcc cagaccctcg
    10681 tatccctgtc gcacaattgg ccctaccttc tggcgggcat cctgaccgcg gccgccctga
    10741 aggccttcct gaagagcgac cgggtctccg ccttcctgct ccgcttccgg ggcgccgggg
    10801 tcttcgcggc caccgcggcc gcggtgacca cgcccttctg ctcctgcggg accaccgccg
    10861 tggtcctggg catgatggcc ggcgcaatgc ccctggcccc ggtggtggcc ttcatggtcg
    10921 cctcgcccct gagctccccg ggagagctgg tctacagcgc aggcctcttc ggatggccct
    10981 tcgcgatctg gttcttcgct tcctcgatcc tcctgggtct cgggggaggc ggcgtggcgg
    11041 cggtactgga gagccgcggc tggctgaagg gacagacccg gttcacgatc gccggcgctc
    11101 cctccccggt gcccggcggc cgggccgcgg cgcgttcccc gatccggaac ccgatcccga
    11161 cctccgcggc ggcctgcgcc tgtgcggccg gccccgcccg cccgtccgat gcgtccgccg
    11221 gtccgtaccg ggacgcgtcc tccgcctgcg gctgcgccgc ggcggcgacc gccccctcgc
    11281 ctcggcccgg gcccctcgtc tcccggaccc tcgagttcct gctcgccgcg gccgaggtta
    11341 cccgggccct cgtcccgatg ttcctgggtt tcgccttcct cgggtacctg ctgaacggcc
    11401 tcatccccgc gtcctggatc tccgccctct tcggggaggg cagggcgtac ggggttccct
    11461 tggcggctac cctcgggctc ccgctctacg tcaactccga ggcctccctt cccctggtcc
    11521 gggccttcct ggactcgggc atgacccggg gggcggtgat ggccttcctg atcgccggct
    11581 cgggcacctc ggtcggcgcg atcacgggcg ccctgacgat cgcccgctgg agggtcgtgg
    11641 gactcgtgac cgcggtgctc tggatcgggg cgatcctggc cggctggggt ttcgacctgg
    11701 ccgccgcggc gggctggatt tgacggaagg gttcaccgaa agggggcgag cccggtacct
    11761 cgccgggttt ctcgggcccg ccccggccgc gtcagggcag gtccgggtag atcgagcggc
    11821 ggacgaaagg cgcggtcagg aagcggccgg cggccttggc gcccgtcagc cagacgatcc
    11881 gggcaccgtt cctgcggttc cggagcaccc ggtccgcgat ccagggcgat acggtctcca
    11941 ccgtgtccga gaggatattg aagatccggc ggacccggac ccactcctcg ggcttgcccc
    12001 gatactcggc ggtgatgagg tccgtggccg tcatgccggg acggatggcc ccgacgatca
    12061 cgccggtgcc ccgggcctcc cgggccgcgg agtcggtgat gtaggccagg gcgcgcttgg
    12121 aggccccgta ggccgtcgtg ccccgtacga tctcccctcc ggatcccagg ccttccatgt
    12181 tgtagacggc ccctcggccc gcggccttca tgcccgccag ggcgaccttg cagccgtaca
    12241 gggcccccgc gcagttcgtg gcgaagatgt tcccgacaag gtccggatcc agggcgtcca
    12301 cgtcggactg gggctggccg atcccggcgt tgttgatcca gacgtccacc cggccgaacc
    12361 ggcccgccgc gaagtcccag agggactgga cgtcccggaa ttcccggacg tcgcaagccg
    12421 ctccggccgc gcgcccggga gccgcggctt cgagtcggcg gaccgacttc gcgacggagg
    12481 ctccgtcccg gccggagatc actacggagc agccgcgctc caggaaggcc gcggccaggc
    12541 cgtacccgat gccccgggtc ccgcccgtga ggacgacgac ggggccggtc gatccgttcg
    12601 attccatgcg tactcccgtt ccccggttcg cggcctcagc agcagccggc ttgccggtcc
    12661 ccggagcatc cgccgtcccc gcaggagggg gcggttcccc ggagcttgcc caggatgatg
    12721 gccgcggcgc agcccacccc ggcccggacg gtcagggcgg ccgcggggca gttcaacgcg
    12781 caggccccgc actccatgca ggcgccccgg ttcgcgatcc gcgctttccc ttcctccacg
    12841 aggaacaccc cgtgggggca gacgtcgacg caggcttcgc acccgacgca ggcatccgga
    12901 tcgaggcgaa ggctgcttcc gttcatcagg tattcgtcca gcatcgcgtc ctcctaggct
    12961 ccgaaggccc gggtcaggac gcccagggcc agtccggccg cgaggctcgc ggccatgggc
    13021 cagaacccct tctccacctc cagcaaggcc ccggtctggc aggtgaaggt ggaggagccc
    13081 gtgaagttca tgcctaggaa ggccgtcacc ggagcggcca ccaggacgcc cgcggcggcc
    13141 gcggacggcg gaaggccgaa gagggcggcg caggcggccc cccaggccgc gcccagagcg
    13201 gcccctttga ccgaaaaggc ccggcccggc agcccgggca ggagggcggg gaaggccagg
    13261 gcgcccaccg gggccaggcc tcccaggagg acggccgcgg gcagggtccg ggaggcccag
    13321 gatccgtcgg ccggcagccc gtagagggcc gccagggcca gccccgcgac ggtcaggggc
    13381 caggagtgga caagctccac cggggccacc gccagccgct cccggagggt gaaggtcatg
    13441 gtgcgcatgc cctcagtctt gatgcgcccg gcggccagcc aggccgggat gtcctcggcc
    13501 cgcacgggcc cccaggccac ccgccagccc gcccggcggg cgacctcggg ggccgagacc
    13561 ccggtcgccc ccagctgggg caggacgagc tcccggtgcc ggaccagggc gcccagccgc
    13621 gtcttggcga tccgcgagac cagctccccg gtgccgaagg ttccctttcc cgcggcgcac
    13681 catacgttga cgcccttggt gtccaggacc aggatccacc cgtcgatgcc ggccaggttg
    13741 gaccggagag tgtcgaagct cagcttgtag ttggcggaga cgaagaccgg ggagtccggg
    13801 ccgggctccc cggccgcgta gagtccggga aggacgcggt aggtcccccg gccgaagccc
    13861 cagcggacct tgagggtgcc ccggcggtcc cggagggaca gacgggtgcg gacgaccggg
    13921 acggtccggc cctcccgtat caggtagcgc tccacccagg gaacctggag ctcccggagg
    13981 gcggcgaatc cggcctggcg ctcggcgggg ccggcggctc tccgcgcaag gtcgatcatg
    14041 acggcctccc ggaccggtcg cagcacgcgg ccgagccctc cgcgaggcgg aaggaccgga
    14101 gggcccgggc caggatcggc agggccttgt ggaggacgtc cgcctcggcg ggcgggaggg
    14161 aaccgagcaa ccgccgatag tagcggtcgc agagtccgtt gatggagtcg gtcttctccc
    14221 ggccggcccc gctcagggac acgatctgcc tgcgccggtt ctccggatcc tcccgccgct
    14281 cggccaggcc ggccctcacc aggccgtcca ccgtccggga cagggtggag gagtcgagct
    14341 ccagggcggc cgcgaggtcc ccgacgctgg aggggccgga ttcctccagg gtcaggagca
    14401 ggtggcattg cgcggaggag actccgcagc acccggtctg ggcctgcagg gccaggtcca
    14461 cctctcgttc gagggtccgc agggtccggc ggaactcccg gatggtcgcg acgctcatcg
    14521 cggttcccga ggggaaaggg ttctcatgga atgcattgtt gtgcaagatg cataaaatgt
    14581 caacagcaat atatgttata tgcattgatt accgtcgttc cgccgaaggc cgatcggagg
    14641 tcctcgcggt tcgccgcttc ccggatggaa tcgcacctcg aggagtccct gaaccggatc
    14701 cgaaaagcga cctgatccgg ttccggcggt ccttcccgcc ccgtattccc ccggccgtgc
    14761 tacaatgccc cggggcgaac cggccccggg aggtaccatg aagcctctgt ccatcgggat
    14821 cgtcggcacg ggattcgtcg ccgagatcca cgcccagacc tacgcgcaga tcgccggacc
    14881 ctccttccgg atcgccggct gcgcctcggg ccgcccggat aacgcccggg ccttctccgc
    14941 gaaatggtcc tacccccgag cctataacag cttcgaggag ctgatccgcg acccggaggt
    15001 ggaagccgtg gacctctgcg cccccaacga ccggcaccgg gacatgatca cggcggcggc
    15061 ccgggcgggc aagcacgtca tctgcgagaa gcccctcacc ggggccttcg gggagggctc
    15121 gggcgccgag gcggcgggac tcctgcccaa gcaggggctc ttccaggagg ccttggccaa
    15181 cgtccgggcc tgcggggagg cggtgcgggc cgcgggggtc gggttcgggt acgcggagaa
    15241 tttcgtctac gccccgcccc tggccaaggc ccgccgccta ctggaccggg ccggcgggac
    15301 catcctggac ctccgggccg aggagagcca ctcggggtcc cacgcggagt acgcccgccg
    15361 ctggtcccgg gccggaggcg ggtccctgat ccgcctggga tcccacccga tcggggccgt
    15421 gctccacctg aagcgccggg agggcctccg gcgggacggc cggcccatcc gccccgtacg
    15481 cgtaacggcg gagacaggca cccacactaa gatgccctcc ttcctcgggg aggcggaaca
    15541 cttcctgaaa gccggttggg aggacgtgga ggactgggcc tgcgcactcg tcaccttcga
    15601 ggacgggagc cacgccacca tccactcctc ggacggagtc ctgggcggag tccggaactc
    15661 cgtctcgata tacgcctcca acgcggtcct ccaggtgaac atcaacccca gcgataccct
    15721 gaaggtctat gcgccgcgtc ccggcatttt cgcgggagag tacatccagg aaaagctgga
    15781 aaccgacggg ggctggaact tcccggcccc cgacgaggac tgggtccggg gctacgacgc
    15841 ggagctcacg gatttcatcg attgcttccg gtccggccgc gagcccgaga gcggccttgc
    15901 gctggcggaa gacacggtcc gggtgatcta cgcggcctat ctttccgcgg aggaggggat
    15961 ccgggtcgac ctgccctgac ggcggatcct ggagttcctc cgccgatccg ctatactgaa
    16021 ggaaatggac ccgagcgcgc gacagggcgg attccggaat gcggtcctct ttttcttcgc
    16081 ggcggcgatc ctcgcgcggc ccgtctcggc gacccccgcc ctcgtcctgc cggaggaggg
    16141 gaagtccgtc gcggcgcggc cctacgcaga atgccgggtg gacgccgcgg gagaggaagg
    16201 gtacgcgcgg gtctcgcggg gcgcagcctt cctgtcctgc gagcggcggt ccttccgctt
    16261 cgacccggcc gagggtccgt ccttatgggt tcgcctttcc atggagcttc gccccggggc
    16321 gccgccggga gccgcctggt accttgagat caacaatccg gccttgagga ccgcccggct
    16381 ctacgtcccc gtttccggat ctaccggagg ggacgtgcgg atcctggccg ccggttggga
    16441 ccccccttcc cgcggacccg tccccttcgg ccggccgggc ttccgtctgc ccccggacct
    16501 cgacccgggc cgtccggttt tcctggaggt ggcctcgagc cacgagatct ccttcgacct
    16561 gtatgtccat tctgaaaccg cccactcccg ccgggagcag gccctgttcc tcttcctggg
    16621 aacctgcctg gggggcctgg gggcgatgct cctgtacaac ggtttcctgt acctggccct
    16681 gggagaccgc cattacggcc tcttcgtcct gtacctcctc tgccagctcc tctaccaggc
    16741 gaccctggcc ggaatcgtcc gatggctctt cccgtcggtc ggcgtcttcc tgatccggaa
    16801 tgtcctaatc cagttcgtcc tcatgaacgc cggcatggtc cagttcgccc gggccttcct
    16861 ggatacaccc cggaacgccc cccggcacga tcgggccttc cgggcggccc tggcggggat
    16921 ggccctcgtg gcgctcatga gcctggcggg gcgcgtacgg gagggaaacc gcctggcgta
    16981 cttcctggcc caggtccaga tgcccctcct gatcaccgcc tccgtcgcgg ccctggtccg
    17041 gggcttccgc cccgccctcc tgttcctgac ctcatcgtcc ctgctcctgg ccgcggccgg
    17101 ggtattcatc ctgcggaatt acggccttct gcccaatacc ctgttcacct tccattccgt
    17161 gctggcgggg accacggccc agtccgtcct gttgtccttc gccctgggcc agcgaatccg
    17221 catcctccgg gaggaagagc gccgcgcccg ggagcgggaa cgccagttcc gcttcgccag
    17281 cgtgaccgac gagctcacgg gactcttcaa tcgccggcat ttcaacgcgg ccctggtcca
    17341 ggccgtgggg gaagccgagc gggagggatc cggcctgtcc ctgctgatgc tggacgtgga
    17401 ccggttcaag gactacaacg acgagtgggg acatcccgcg ggcgaccggg tgctggcgga
    17461 gctgggggcc gtgatccggg ccagcctccg ggattgcgac gcgccctgcc gctacggggg
    17521 cgaggagttc gcggccatcc ttaggggggc ggacctggcg accgcccggg ccatcgcgga
    17581 gcgcatccgg gaccgcttcg cccggtccgc cttcgagccc cggccgggca agactgcccg
    17641 ggtcaccgtc agcatcggcg cggcccgcct gggaggcggg gaaagcgccg aggacttcct
    17701 ggagcgcgcg gaccgggccc tctaccgggc caaggacgcg ggcaggaacc gggtggagtc
    17761 ctgaaggatt tccgattccg tacacgaaaa cagtcccccg tccgtcgata tgcatgaaga
    17821 ccatgaagaa ccaagccgag tccgacgaaa cggaatcccg aagacgcctc acggaagcct
    17881 accgggccga gaagccccgc ctgctggcgc gcctgcgggc cgcggggcgg acctgggagg
    17941 aagccgagga cctggtgcag gacgtctatg ccgagaccct ggagggcctg cccctggtgt
    18001 ccggcatccg gaacctgccg gcctggatca actggctttt cacccggcgg gtcacggacc
    18061 tgtggaggcg ggagaaggtc cgctccgcct cgagggtgga ggtgaccgag gagacccttc
    18121 gcgagatcat agccgggacg ggcctggatc ccctggacga gtacgtgcgg gacagcctcg
    18181 cggaagccct gaacgacgcc ctgcgggcct tgcccgcccc ccagagaaag gtgctggagg
    18241 cgcaggtctt cgggggcctc acgttcaagg agatcgccga gacgacgggg gagaacatgg
    18301 ataccctcat ggcccggaaa cggtacgccc tggcgaacct ggcccgcgcc ctgaggcact
    18361 ggatcgagga atgaggagga agactatggg tgagaggatg aaggacgggg aatgccggcc
    18421 cgacgcgtgg tgggaggatc gcagcgttcc ccaaaaggtc ctgatcgtga tcggcttcgc
    18481 gatcctggga gccggattcc tggtcctgat cggctgggtc gtgatgtccc tctggaactg
    18541 gctcatgccg gccatcttcg gcctgccgcg cgtcggctac tggcaggcct gggggctgtt
    18601 gatcctgagc tcgatcctgt tcaaaggatc cgggcacggg gacaagggcg gccgggcgga
    18661 ccgcaaacgg cggagggagc tgcggaaatt catgaaggaa ggcgcggacg gcccggacgc
    18721 cgagggcgga accgccgagc cggggggcga agccacgtga aaccgggaat ccggctctga
    18781 aagaacggcg aggccggggt ggaagccccg gcctcgtcgt atcttgttca tccgtccgga
    18841 gcgctagacg gggaaccgga tggtccagcc cgtgcccccg gatcgatcca gggaccagga
    18901 tccccggatc tgctggcaga gggaccggat cagcaggaaa cccagggtgg agctccgttc
    18961 gggatcgaaa tccggcggca gccccgtgcc gttgtcggaa agctccagaa ccacgaagcc
    19021 ctccgtatcc cgggcggcac ggacccggat gcgcggggcg gggtggtccg gcgggaacgc
    19081 gtatttcagg gtgttcgtca ggatctcgtt catgatcagc ccgcagggta ccgccttgtc
    19141 gatatccagc tcgatccggt ccatccggag ttccagttcc ggcgcggagg agcccaccga
    19201 ataggtggag gcgagccaat ggacgatcgt ctccccgtac tccgccaggt cgatatgcgc
    19261 caggtcccgg gagcggtaga gcagttcatg gatgcccgcc atgacctgga tgcggttgat
    19321 cgaatccgag aagagccgga cctgctccgg atcaccggag gcctggagag aaagtaggct
    19381 ggtgaccacc tggaggttgt tcttcacccg gtggtggatc tccttgagca ggatctcctt
    19441 ttcccggaga ctggccgcgg tccgctccgc ggcctgctcg gattccgtca tgtccacgta
    19501 cgtcacgacg aatccctcgg cgatcagggc ggcttggccc accacgctcc gacaggagcc
    19561 gtccttgcag cggagccggc cccggaaggg cgcctccgtc cgctctcccc ttcccagtcg
    19621 gtcgatgtac tcctgccaga aggtccggac ttcctctcga taccggggat ccgggtagac
    19681 ggaggaccac cacttctcgg cggtgtcgat gtcctcccgg gcccagccga agacggacga
    19741 gaaggcgcgg ttcatcaact ccatccgctg gtcgcgggcc agcaggatca ccggaacggg
    19801 catgaaatcc agcgtttcga ggagccgccg ctcgttctcc cggagccggg tttcccgctc
    19861 cccgatggcg cgtcccatcc ggacgagatt ggacgccagc agggagaaat cccggaaacc
    19921 cttgaatgtc aggacggcct ccgttcttcc ctcggcgatg tcctgggaga accgggccag
    19981 ggcggcgagg tcccggcgca ccagccggac gctgcccagg atgaggggca gggaaaggac
    20041 ggcgtaggcg accaggacca ggacgaggga gatccggaac gcgagcaggg aagcgaagac
    20101 ccgggcggag ggttccgaga cgatgaccgt ccacccggag gggccggacg tggaagccca
    20161 ggagaggagt tcttcggaac cctcccgtct cgtgaccagc gtgcttccgg acccggcttg
    20221 tatatcccgt tcccgaaacc agggttccag accggcgatg tcctcccggc cggacagggg
    20281 aaggttcgag ttgtgggcca cgaaggctcc ccggtcgtcc acgacggaga tggaccggtt
    20341 ctggctcgag ggcagactcg aaaggaagtc ccccaggaag cccaggtcga tatacccgat
    20401 ggcgaccccg tccgggatcg gatgggccaa gaccgcggag ggagtgtacc ggattccgga
    20461 aacgaagggc cgggacacga agtccgcccc ggtctcgaac gggatccgga agtactcccg
    20521 aaaggagaag tcgaaccccg catccccgga gcccatggag gcccgggaga cccggcccga
    20581 ggagtcggtg aacaggaccg tgtcgaaggc ggggtaggct tcgtgaagga ggtcgacccc
    20641 cttcggacgg aaatccctgg aacgcaggag cgagagcccc atgaaatgca ggtccaggaa
    20701 ggccgagatc tcgtgggcca cgctggcggc caggagcccg ttcgattcct ccgcgtggcg
    20761 ggtcaggtcc gcccgcacga agcgctccag ggatactccc atgatggaca gggaggaaag
    20821 gacgatcacg ctgaacaggg tgccgaggaa ggacgtgacc cggaatcgtt cacgcacggg
    20881 tcaatccgcc cggcccaaca cgacgaaccg gcctccgatg atccgcttca ggtacagggg
    20941 tcggaaggcg tccccgaact cgtccatgcg tatggtgccc agggtgcctt cgaaggaccg
    21001 aatcccccgg agggtggacc ggagcttccc ggggtctccc cgggcggcct tcaacccctc
    21061 ggtcaggaag atcagggagt cccaggtctc cagggctcca taccccggag agacgccgaa
    21121 ggttttcttg tacagtaccg aaaggggata ggtggaggcc cggtccaggt cgacctggtg
    21181 gacaaggacg gccccctcga cggcctttcc tccgtaggtg atgacatcct cgttcccggc
    21241 ccaaccgctc aggtacaggg gcttccggat gccctgccga accaggttct gggcgatcgt
    21301 gcccgaatcc agggagctcg ccacgatcat gaccgcgtcg aagccgggat cccgggcaag
    21361 ggcctcgaga cggccgtagt cgatctcctc caggcggtta taaaggacag tgtccacgag
    21421 cgcgacttcg gggacgagcc cggatcggta ggtctcgtag tacggccggt tgtaggctgc
    21481 cgccaggaac aggacgcggg aatgcctcga ctccctcatc ctccgggcca ggaccgcagg
    21541 gtcccgggcg gaggtcatga tggtgcggaa gaagggatcg tccttgcccg acagctccgc
    21601 ggaggtggac gtcggactga ccgccgggat ccccgtttct tccagcacgg gcagggcctg
    21661 cagggccgag gagctggaga agaacccgag gatggcggcg gctcccgcag ccttcaggtc
    21721 ccgcaccgcg tcgggggcgc gggagtcgtc gctggccagg tcccgcagtt ccagcctcag
    21781 cttccgtccc ccgatcccgc cctcctcgtt cagcctgtcc acgaagagga aggccgcgtc
    21841 ccggccctcc gtgcccagca tgtagtccat cccggacagg ctggccacga acccgatggt
    21901 gatctccccc cggggggcgc aggagacggt caggacggcc aggaggagcg cggcaagccg
    21961 cgcgggggcg aaccttcgca tcatgggtca cctttcctga gcgaaagagt aaagcgccgg
    22021 ccgcaatcgc gcaaggggag gcgggcagca ccccgtggcg gaggcctcga agggcttccg
    22081 tgactccggt atactcggac cgggcccgcc ccgaatccgg tcggccccgg gaggacggtt
    22141 cgatggacga cgtgttgttc cggagggtgg tccggctggc cgacgatttt accgtctccg
    22201 cggattcccg gctgcgctgg atgtgggggg aagccctcct gggtttcgcc ctgctggagc
    22261 tggaccgcgt cctcggggag gaccggtacc tgcccttcct gagggccttc ggtgaccact
    22321 gggcggcccg ggatccccgg gtggaccagt cggacacggc cgccccgggc ctggtgacct
    22381 acgggctgca gaaaaaactc ccaggccggg gctgcgaacg gctgaccgcc aaggtcctgg
    22441 actatatccg gaacgccccc cgggtcctgg acgactcgcc caaccacctg ggctcctccc
    22501 cggagggccg gctctacccg gtctcgatct gggtggactc cctcatgatg ctgtccgtgt
    22561 tcccggcggt ctacggcgca gagaccgggg accgggagct cctggacttc gcggcccggc
    22621 agccccggct gtacgccgcc cgcctccagg atcccgaaga cggcctgtgg cgccacagct
    22681 ggtgggtgag ggcggcccgg ccccatcccg ccggtcgagt ctactgggca cggggaaacg
    22741 gctgggtcgt cgcctcgctt ccccgcatcc tggaccgcct gcctcaagac catcccgagc
    22801 gtccgggcat cctggacatc ctttcccgga ccagccgggc cctcctgccc ctccagcggc
    22861 ccgacgggtg gtgggagacc gtactccttc cgcccgggaa gacctaccgg gaatccagcg
    22921 ccaccgccct gatcgccgcg ggctggctgc atgccgccgc ggcggggtac ctgccctcct
    22981 cctaccgcgc ccccgcggaa atggccttcc ggtccgtggt cgactccctg gccgcggggc
    23041 cggacggccg cctggccatg cccgagatct cggcccccac gatccccctg ccggtcttcc
    23101 cccgcctggg gtacaggctc gttccccggg gctcgaattg gacctacggt atagccgccc
    23161 tgatccttgc ggcggtggcg gggaggaagc cgggcggggc ggtagctcgg tagccgggaa
    23221 gccgtccgaa cggggagtcc tgcatggata cggaccgggt ggtgttccag aataccggcc
    23281 cattcctgag gtaggccggg ccttacaagt cctccttaaa agtaggcagg tttcagctac
    23341 ttggatccgc attccgtttg gcccggcccg gcggaggctc cttccttgag gatgtcgtcc
    23401 ggtgaacggt tgctaaaaag cccggccaaa aataatgctt gacaattccg ggatgcggcc
    23461 tattatggct ttaggatatt aaatcatgga ttaaggatat tggatccaaa acctgttcag
    23521 gatgccagcc aagatatctt cgtatatacg gctaacgtaa cgggaggaaa gtatgagaac
    23581 gtggaagaaa tgggtcctgt tgttgctcgc ggccgcggtc gtccctgcgg tctgtttcgc
    23641 ccaggccaag aagtacaacc tcaagctcgc gcatgtcgcc ccggtggagg aaccctatgc
    23701 ggtggccgcc ctgaaattcg ccgaactcgt caaggcggcg acggacggca acgtgaacat
    23761 cgagatcttc ccgaacagcg ttctcggaag ttcccgggac ctggtcgaag ggctccagat
    23821 cggaacggtg gacgcgacac tctgtaccgc cgccgtgctt gcgaacttcg tgccgaaatc
    23881 ccaggtcatc gagcttccgt tcatgttcaa gagccgggag catgtctaca aggtggtgga
    23941 cggccccctg gcggacaaga tctacgaggg cgcagagtcc aaggggctca agatcgtgag
    24001 cacctgggag aacggattcc ggaacatcac gaacaacctc cggcccatcg tgactccggc
    24061 ggacatgaag ggcatcaaga tccgggtgat ggaaagccag atgtacatcg acctgttcaa
    24121 ggccctcggg gcgaatccta cccccatggc ccggggcgag ctcttcacgg ccctccagca
    24181 gaagacggtg gacgcccagg aaaaccctat gggccagatc tacacctccc ggttctacga
    24241 ggtccagaag tacgtcagcc tcaccggcca ttcctactcc cccgaggtgc tggtattctc
    24301 cctcgccacg tggaagaagc tccccaaggc ctatcagacc gctattgaaa aggcggcggt
    24361 ggaggcccgg gacttcaacc ggaaactctc cgcggagaag gacgacgagt tcatccgcct
    24421 ctgccaggaa aagggcatgc aggtaacccg gttgtccccc gcgcagatcg ccgagttttc
    24481 gaagctgatg ggaccggtct gggaaaagta ctacaaggtg atcgggaagg acctcatcga
    24541 caagatctcc agttccaagt agcgagtctc ccgatcccat tccgacgagg atcggcccgg
    24601 gtcggccggt cctcgtcccc cctcggagta aaccctatga aggcctacaa gaatctcctg
    24661 gatgcggtta atcggggaac tttgtggatc gcgatcctgg cggccgcggt gatgtcggtt
    24721 ctcgtcatcc tgcaggtcgt cttccggtac gcgatccaga gttccctcag tttttcggag
    24781 gaactcgcga ggtttctgtt cgtttggact gcgttcctcg gttccgccgt ggccctggcc
    24841 aagcgagccc atgtaagcat cgagatcgtc gtggcccatc ttccgcggaa actgaagagg
    24901 ggagcgatcc tggttaccaa cgggctcgga gcgatcttct tcctgatcct gatcgtgtac
    24961 gggatcttga tgaccatccg gaccttggat cagacctccg cggccatgga gctttcgatg
    25021 ggcttcgtgt acgtggtcgt tcccctgtcc gggctgatca tgctcatgaa tctcgtctac
    25081 aacggatacg aggaattcat gaatcccgac atcatcaaga gtgagggggt ggaatgatga
    25141 ttcctctaat cctgttcgga tccctggcga tcctcctcgt cctgaacgtg cccatcgccg
    25201 tggcgctggg gctggcgacg accttcgccc tcctgcagtc cgggctcccc ctcctggtgg
    25261 tcgtccagcg gatggtgacg gccaacgatt ccttccccct cctggccatc ccgttcttca
    25321 tgctcgccgg atcgatcatg acctacggcg gggtttccaa gcggctcgtc gcgttcgccg
    25381 acgcccttgt gggctggctg acgggcggcc tggggatcgt ctcgaacgtt tcgggaatgt
    25441 tcttctccgc catctcggga tcctcggccg ccaccacggc cgcggtggga tccgtcatgt
    25501 tcccggagat ggagaagcgc ggatacgacc ggtccttctc cgcggccatc atcgcggcgg
    25561 ccggagagac gggcatcatc atccctccga gcgttccgat ggtggtgtac ggcgtgatcg
    25621 ccggcgtctc gatcggggac atgttcctgg gaggattcgg cccgggcatc ctgatgggca
    25681 cgacgatggc cgtgctcgtc tatttcgtgt cccggaggca cggctataaa ggatcgtcct
    25741 tcggcggtct gcggaaactc ggcaagtcct tcctggacgc cctctgggga atcctgatgc
    25801 cggtgatcat cctgggcggc atctacggtg gcatcttcac tcccaccgaa gccgcggcga
    25861 tcgcgtccct gtacggcgtc ctcatcgggt tcttcgtgta caaggacctg aaggtctccc
    25921 acttcccgaa gatcatgcag agcaccatcg tgagcaccgc ggtcgtcatg ttcatcatga
    25981 attccgcggg gctcttcggc tggctcatcg cccgggagca gatacccgac gccttcgcgc
    26041 ggttcttcgt gagcatttcc tcgaacccca cggtgttcct tatgctcgtg aacgtcctcc
    26101 tgctcttcac cgggatgttc ctgaacgcct ccgcggcgat cacgatcctg gcccccatcc
    26161 tggttcccgt cgcgatccag ctcggcatcg atcccgtctt cttcggcgtc atcgtggtct
    26221 gcaacctcgc gatcggctgc ctgacgcccc ccgtgggcgt ggacctgttc gtcgcctcga
    26281 cgatctcggg ggtttccatg gcccgtcttt cccgggcgat cgtcccgttc ctggtggtgc
    26341 tgatcgcgga cctggtcgta ctcacgtacg tcccggcgat caccatgtgg ctacccaatc
    26401 tcatgagata ggcgaggaaa cctcgcgata ggagcgacca ggatggacat cgaacgattc
    26461 accgacatta ttgcccggtt cctcgcggtt tcgggaaaga tccttccgga cgacattgtc
    26521 aagaagctcg cggagctcaa ggccgccgag cacatgcccc tggccaaatc cgtgtacgat
    26581 tccatgagcc ggaaccagga cctggccgcg agtctggacc ggccgtcctg ccaggacacc
    26641 ggggtggtca atttcttcgt gagcgcgggg gagaactttc ccgggctcgg aaaaatcgcc
    26701 ggagccctgc gggaagccgt ggtccgggcc accgccgagg cgccgctgcg ccacaatacg
    26761 gtggccatct tcgacgagaa gaacgacggg acgaatgtcg gggagagggc gcccttcgtc
    26821 cactgggaca tcgtgcccgg cagcgacaag gtgaaggtgg agacctacat ggccggcggc
    26881 gggtgcagtc tgcccggccg cgccatcacc ctcatgccat cggccggata cgagggcatc
    26941 gtccagtacg tgttcgacac gatggtcgac tggggcatca acgcctgtcc gcccctgctg
    27001 atcggaatcg gcatcgccgg ctccgtggag aacgcctcga tcctgtccaa gcgcgccttg
    27061 ctcaggccac tgggatccca taacccgaat ccccgggggg cggagatgga aggcctactc
    27121 gaggacgggc tcaatcggct cggaatcggc ccccagggct tgagcggaag ctcgagcgtg
    27181 atgggcgtcc acatcgagag cgccgcccgg catccctcca cgatctccgt gggaatcaac
    27241 gtgggctgct gggcgcatcg gcgaggcgcc atcgtgctcg acaaggattt gagctacgag
    27301 atcctctcgc acaaaggagt acggctgtga acaaggtgtt gcgcactccc atttccgatt
    27361 ccgatctcga ggacatccgg atcggggacg tcatctacat ggacgggatc ctcgtgacct
    27421 gccgggacgt ggcccaccgc cgcctgatcg agttccatag ggaactgccc gtggacctca
    27481 aggggctcgc catcttccac gcgggcccca tcgtcaagaa actgcccgac gggaagtacc
    27541 agatggtttc catcgggccc acgaccagca tgcgcatgga acgcttcgag aaggagttcg
    27601 tccgtcagac cggggtcaag atcatcgtcg ggaagggggg catggccgag gagaccgagg
    27661 aggcctgccg gacctacaag gccctgcatt gcgtgttccc cggaggctgc gccgtgctcg
    27721 ccgcgacgga ggtggaggag atcgagcggg tggagtggat ggatctcggc atgcccgaat
    27781 ccctctgggt gaaccgggtc aagggcttcg gtcccctcat cgtctcgatc gacgcccacg
    27841 ggaataacct tttcaagaaa aacaaagcgg tgttcaacga gcgcaaggac cgtctggtgg
    27901 cggagatctc cgagaaggtc cggttcatcc agtagcggga acggcgtcga aggccccggc
    27961 acggccggag gcttgcggcg ccgtattcat atccgaggtg aagcgacgta tcgaaggagg
    28021 catcgaatga agagggtcgt ttgtctggca ttggcggcgc tcgcctgcgt cgggttctcg
    28081 ttcgcgaacg ggacccggga ggcggaagac gggactgcgg tcctgccgct gcgggatccc
    28141 gttcccacca agtccggatg ggtatccggc gtggtcaacg gaaaacccgg gaaggaagtc
    28201 atcagttacc gcggcatccc gtacgccgct ccccccgtcg ggaatctccg gtggaaggct
    28261 ccccagcctg taacgccctg gacgggcatc cgggagtgca ccggcctgac caagtggccg
    28321 ccccagagct tcccgaccgt ggagcggtac gggtcgatcc cggaatcctt catgagcgag
    28381 gactgcctct acctgaacgt ccacgtcccg gtccggacgg acggcaagcc acttcccgtc
    28441 atggtctggc tccacggagg cggtttgacg aaccagtccg gcagccgcga catctacaac
    28501 atgcccttcc ttcccgccgc cggaaacgtg atccaggtaa gcgtcgtcca ccggctcggg
    28561 ccgatcggct acctcgccca tccggccctc tccgcggaat ccccgcaggg cgtgtccgga
    28621 aattacggaa acctcgacat cctggcggcc ctcaagtggg tcaaagacaa catcgccgcg
    28681 ttcggcgggg atcccgggaa cgtgaccctc ttcgggcagt cgggcggcgg aacgaagacc
    28741 gcgatggtcg tggcctcgcc cctcgcgaag ggcctggtcg acaaggccat aatcatgagc
    28801 ggccgcgtgg acggcacccc cctcaaggag gccgagaaga tcggactcgc gctcgccgaa
    28861 aagctgggag tgaccggcac cgggccggaa gccctggccg cactgcgtgc gctgaagtgg
    28921 caggacatca tagtgacgag cctgcaggag aagtattccg cggaccacac catcgacggg
    28981 tgggcccttc cggatgccgt atccgcgatc ttcaaggacg gaaagcagaa cgacattccc
    29041 ctcatgatgg ggttcacgga gtccgacatc cccgactacg ccatccaggg cgtatcgacg
    29101 gtggtttccc agatgaagat gctcaagtcg ccggtctatc cgtacatgtt cacctacctc
    29161 cccaagaatt ggcggaagga tggggccaag gcttggcatt ccctggacgt cggctacatt
    29221 ttcgggaacc cgcgcaagat agcgccggtg aatttcggcg actacggcaa gcgggcgggt
    29281 gcggtcaccc cggacgccgg tttcggcccc gcggatgacg cgatgtccga gttcatgatg
    29341 cagacctggg cgcagttcgc gcgtaccggg gatccctccc tgcccggcgg cctgaaatgg
    29401 ccctcgtatt cccgggacaa ggatcagtat gtcgcgctgg atacggtacc ggagatccgg
    29461 accggatatt ccgcgttcgc cgccaagtag gggcggcgaa gcggccggat ttcccgactc
    29521 gttctagctg aggaccacgc ccttcgtgtc cgggttttcc cgggtcgcgg agggcgtttt
    29581 cgtcttccgg gcgaacatcc ccgttccctg cgggaacctg gtcccgcgcc gcgcccggct
    29641 cggtcgattt acttaatcaa ggatatatga tatagtctcc acgtgcgagg ataacctctt
    29701 atgttgaagc aaattagcat cctaccgatc cgcgagaggg tcgcctccga gatccggaac
    29761 gccatttttt ccggcagttt cgctcccggg gaggaactcg gccaggatgc catagccgcg
    29821 aagctgggcg tatcgcggat gcccgtccgg gaagccctgc agatcctcgc cggcgaggga
    29881 ctcgtggagc ttcgggtgaa ccggggagcc atcgtcaagg acgtttcgac ggaaagcatc
    29941 cgggagcatt tcgagctgcg gctcatcctg gaatgcgagg ccgcggcccg cgcgtgcggc
    30001 cggatcacgg atttctcgga actcgactac atccatgaag agcagagaaa ggcgatcgag
    30061 aaccaggatg tcgcgaaagc gaacatgtgc aaccaggcct tgcatatgta catctgggag
    30121 cattccggga acaagaaact gaaaaacatc ctggtgcagc tgtggaacgg tctttcgatc
    30181 ggaaccgtcg ttcgtccgat cgaccatatg cgactttctt tcggagagca cggcgagatc
    30241 atacaagcca tgaaatcacg ggacgagaac agggcccggg ccgtcatggc cgcgcacctc
    30301 aagcgaagca tggagaacat gatccagcgg cctacggccg aaaaagcaac agaatcccct
    30361 tcccagaccg cctgaggccg tgtacggctg cggaacgcaa aacgtgatcg gtactacgtc
    30421 gccggcggat acggctccta aattccgatc cgggcgatac aggttgcacc caggcgcgct
    30481 tcggaatagc ctgaaaagct ctcaccaccc gttcctgtgg acccgggacc cgtcgtaccg
    30541 gtccaccggg gccggcttct gggccggctt gccgacgggg atgaccgaga acggccggat
    30601 cttttcgggc aggccgaggt agccgcggac gaacccttcg gacccgggca gcacggcgct
    30661 ccagacggct cccaggccca gggaatgggc ggccaggagg atgttctggc tggccgcggc
    30721 ggcgtcggaa tcccccgcac gggtcaggcg ttccagattc tcgtccacgc agacgaccag
    30781 ggcgaggggc gctcctttcg gcgtattcgc caatccccgg aggagacctt cgagccgttc
    30841 gccctccagg acgacgaagt gccagggccg ctggttcatg gcgctgggcg cgtggaaacc
    30901 ggccttcagg agcgcttcaa gggaatcggg cggtacggga tccggaagga aggagcggac
    30961 gctccggcgg gtcaggatgg tctcgatcat ggtgcctcct gctcctgaga atatactggg
    31021 gcgggctcgg gaaggcgaga tccgcggcgg ccggcctcga ggccgtgtgg aggacttcgg
    31081 ggcgggttcc ttacaggctt cttccggagg ccgattcgaa gaaccgggcc aggtgccgaa
    31141 ggtagccctc ctccccgtgg taggccgccc gatacgcagg atcggggagg atcggagggc
    31201 cccggtcggc gacgacggcc gccacccggg cggcgaactc ctcccgttcc tccgaggaga
    31261 tccgggccga ggccgcggcc acggcggcgc ccgccgcggc gcccgcccgg ccgatccgcc
    31321 gcacgggccg gtcccagatc gcggccaccc gtcggagtac ctccacgctt ccggctcccc
    31381 cgccggccgc cgcgattccc gcggcggggc ccgccgggtc cccggaaaag ggccgggacc
    31441 cgagccaaag gagcccgagg gccgagtcga ccgcgccgga ataatcctcc gcgaaatctt
    31501 tccggccccc gaggttcagg ggcggggatt ccgggaagga ctccggctct ccttgaagga
    31561 tcctcagcac ggatccgggg ggcgtcgagg ccagggcccg ctcggatgcg gagaagtcgt
    31621 cccccgccag gccgtgggaa atccgcaccg attcccagga gagggaaccg ttcgtccggc
    31681 agccgaagag gaagggacgg ccgaggccgt cgtacatcgc gttggcgctc ggatggggcc
    31741 ggccgccttc ggacatcagg acgaagctgg tgcccaggga caggagccct cccggagaca
    31801 ggaccttggt ctggggattg tcccccgacc cggccgccac gaccgcgtcg tccggaattc
    31861 cgaaacgttc cgtgaagtac cgcgccgccg tcccgaccac gtgcagggga tgggccaggg
    31921 ccggaagccg tgcgtgtagt ccctccgccc ctcccggaag acccgccgcc gcggcttcca
    31981 gaaggacggg gctccaccgc ctggccgccc agtccatcag gctcgtgccc gccccgttgc
    32041 cccagtccgc cggggcgtcc ggccgtccgg cgagcaccga ggccaggaag gaggagatta
    32101 ggtggatccg ggagcaggcg gcgtaggtcc ggggttccct cagggcccgc cggcgcagga
    32161 cggccccggt gaagcggacg gggctgtccg acccggagag ggcggtcatc gcgtcggcgc
    32221 cgcccgcggt cgtcctcagc tccccggctt cccgggcggc gttcgagctc atccagatgg
    32281 gggctcggcc cgaggccagc gcggaagcca ggtgttcggc caagcccggg gacccgggct
    32341 ttccggcccc cgcgagacgc agggcccgga aggcgccctc cgcccgggga ttcagccaga
    32401 cctgaccgtg ctgctgggca gagaccgcga cggccgcgac ccgggacaga aggtcccggg
    32461 gaaggtcgga gaggaccgcg tccaaggccg ccaggaaaag cccgacgggc tggtccgctt
    32521 ctccctcctc ccggggcgga aggaggaact cgtttccttt aagaccgaac cccgcgagcc
    32581 ggggatcccc gcggtaggcg acccgggcct ccgcggccag ggccggggac tcgaggtcga
    32641 tgagcaccgc cgcggtgccc tgggtgctcg agtcgatgcc gagcgccaaa cgcggagccg
    32701 tcatggatcc tccctacccg cccgtgccgt cagaggtacc ggttcacccg ggactccagg
    32761 aactcctggc cgcccgaagg gagatcgaag tcttcgatat ccagggcgaa ggactcgagg
    32821 ctcccgaagg attcccggcc cgcctcgacc gccgctccca cgccttttcc ccagagcgcg
    32881 tagcgctccg ccagggggcg gtccagggct ccgtcggcga tcatgcgccc cgccgctttg
    32941 tagccccggg ccatggtgtc catcccggct atgtgggcga tcgcgagatc ctcgggccgg
    33001 tggctgcccc tgcgcacctt cgcgtcgaag ttgagacccc cggagtggag acccccgttc
    33061 ttccgcacct cgtacatggc cagggtcgcc atgtacaggt ccgtggggaa ctggtccgta
    33121 tcccagccca gaagctcgtc cccctggttg gcgtcgatgg aacccaggat cccccgttcc
    33181 cgggcgtagc ggagctcgtg ttgcatggtg tgtcccgcca gtgtggcgtg gttcgtctcg
    33241 atgttgagct tgaagcggga ggacaggccg tattcctgga ggaaggccca gcaggccgcg
    33301 gcgtccgagt cgtactggtg tttcgtcggc tccttgggct tcggctcgat gagaagctgt
    33361 ccatcgaagc cgatctcccg ggcgtagtcc gcggccatgt ggaggaaccg ggccagatga
    33421 tcctgctctc tcttcatgtc cgtgttgagc agggtctcgt agccctcgcg gccgccccag
    33481 aagacatagt tttgaccccc gagttccaaa gtgacttcca gggccttctt cacctgggcg
    33541 gcggcgtagg cgaagaccct ggggtccggg ctcgtcgcgg ctccatggag gaagcgcgga
    33601 tggttgaaga ggttggccgt gccccagagc acccgggtgg gactcgtccg catgaggtcc
    33661 ttcgtcaggg cggtgatctc gtcgagcttc cggttcgtct cccgcagggt atccccctcg
    33721 ggggcgatat ctcggtcgtg gaagcagaag aagggaatgg agagcttttc cgtgagctcg
    33781 aacacggaac gcatccgcag acgcgcgacc tccatctcgt cgtccacgcc gtcccagggc
    33841 cggaggaggg tcccgtcgcc gaaggggtcc tttcccgtgc cggtcatgga gtgccagaag
    33901 gccatggaga agcgaaggca ctccttcagg gtcccgggct ccggagaatc cggagtccgg
    33961 gcggccgggt cgtagtgctt gaaggccagt tcccggtcgg aatccggacc ctcgtacggg
    34021 attttcggta tcgaggggaa taggtcgttc acggttgcct cctcgctgcg gtgcgcgaat
    34081 ccttaacgat actagagctc cttcccggga gggtaaagat cgggatttga ccgaatatat
    34141 ccgaaattga tattattccc gaacaccacg aaccccgcgg aggcccagga tgcgtttcga
    34201 ggacatcgtg ttcgtatacc acctgcggga cgagcgccag atcgcctggc acgggaggcg
    34261 gcatgcccac cgggagggag agtacgagct gcactacttc atctccgggg aggggagctt
    34321 ccggaacggc tccgggacct ggaccatccg gcccggaagc ctgcacatga cgcccccggg
    34381 ttcggtgcac cagatcctgg ccacgaacct gcgcaagccc atcacctatt tcgccgtgct
    34441 gttcagcgcc tccggggacc aggagctttt cgggctcctg gaccgcgcgg tggacgccat
    34501 ggcccgaccc gtcgacatcg gcacctccca ccggttcttc ttcgcggacc tgctggagcg
    34561 cagctcctcg ggccacgcgg acctcgtgga agccagccgg cattccttcc tgtccttcct
    34621 gtacggcctg gtggccgggg ccccatcctc ctacggtgcg ccggagaact cgcacgtgga
    34681 gaaggcgatc gcgatcatgc aggcttccat tgaagacaac ctcgacctgg attcgctctc
    34741 cgggcggctc ggggtgtccc gtgagcacct ggtccgggtc ttcgcccagc gcatgcgtat
    34801 gccgcccatg cgctactact cgcggctgaa gatcgaggcg gctcgggcca tgctgtccag
    34861 caccaacctg ggcatcggga cgatctccga gaagctcggg tacgagaacc agttcggatt
    34921 ttcccgggcc ttcaagaacg tcgtcgggtt ctctccgacc gaataccggg ctcggtgcct
    34981 tcagaaggcg gacttcgccc actcctgatc aatatcaatt tcggatatgg aaaatcaatc
    35041 cctcccatag tgtgccgggg gaaacgcgcc tatcgtcacc ctatagcgcg cttcgatcga
    35101 atcccaagga ggcacacaag atgaagaaag gtatcctgat tctcctggcc ctcgcggtcg
    35161 tctgctcttc cgcgttcgcc caggggatgc tcatcggcgt cgccatgccg acgcagtcgc
    35221 tccagcgatg gaaccaggac ggcgcgaaca tgaaggccca gctcgaggcc aagggctacc
    35281 gcgtcgatct ccagtacgcg aacaacgata tcaataccca gatccagcag ctggagaaca
    35341 tgatcaccaa gggatgcaag gtcctggtga tcgcgtccat cgacggctcg gttctctcgg
    35401 acgtactcaa gaaggcccag gccggcggga tcaaggtcat cgcctacgac cgcctgatca
    35461 tgaacacccc gaacgtggac tactacgcca ccttcgacaa tttcaaggtg ggggtcatcc
    35521 agggcaagta catcgagagc gccctcgggt tggacaaggg gaagggcccg ttcaatatcg
    35581 agctcttcgc gggatccccg gacgacaaca acgcgacctt cttcttcaac ggggccatga
    35641 gcatcctccg tccctacctg gacagcaaga agctcgtcat ccccaccggc cagaaggact
    35701 tcaccgtaat cgccatccag agttgggagt ccgccaaggc acaggcgcgg atggacaacc
    35761 tgatcgccgc caagtacgcc gggagcacca agctcgacgc cgtgctctcc ccgaacgaca
    35821 gcctggccat cggcatcgtg gcctccctga agaacgtcgg ctacggtacc gcccggaagc
    35881 cctatcccat cctcacgggt caggattgcg acaaggccaa cgtcaaggcg atgatcgcgg
    35941 gccagcagtc catgtcgatc ttcaaggaca cccgtaccct ggccgccaag gtcgtggaga
    36001 tggtcgaggc catcatgcag ggcaagacgg ttcccgtgaa cgacaccaaa acctacaaca
    36061 acggcgtcaa ggtcgtaccg tccttcctct gcgaccccgt gtacgcggac aaggccaact
    36121 acaaggccct cctggtcgat tcgggctact acaccgaagc cgatctcaag tagaatagcg
    36181 caacattcca ggtccggacg cccgcacgcc tcatggcggc gggcgtccga atcgcggctc
    36241 gcatatggat taggagaacg cggcagtggt cggcaacatc ctcgagatga ggagcatcac
    36301 gaagctgttc ccgggcgtcc gggccctttc cgacgtgaac ctctcggttc gtcgggggga
    36361 gatccacgcc ctggtcgggg aaaacggcgc gggcaagtcc accctcatga acgtactcgg
    36421 cggcgtatat ccccacggga cgtattcggg acagctcgtc ttcgaggggg aggagtgctc
    36481 cttccggacc atccacgaca gcgagcggaa gggcatcgtc atcatccacc aggaactggc
    36541 cctggtcccc tatctgtccg tgggggagaa catcttcctc cggaacgaac gctcccgggg
    36601 cggcgtcatc gactgggaca agacccatcg ggacgcggcc aagctcctgg agctcgtggg
    36661 gctcaaggaa ggcacccgga cggcggtgaa ggatctgggg gtcgggaagc aacagctcgt
    36721 ggagatcgcg aaggccctct ccaagaacgt gaagctcctt gtcctggacg agcccacggc
    36781 ctccctgaac gaggacgact cggagcactt gctcgggctc ctgctcgacc tcaaggccaa
    36841 gggcgtcacc tccatcctga tatcccataa gctcggcgag gtcg
//
LOCUS       DAOTEI010000013        36522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_776_length_36522_cov_5.673787, whole genome shotgun sequence.
ACCESSION   DAOTEI010000013 DAOTEI010000000
VERSION     DAOTEI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 36522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36522
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_776_length_36522_cov_5.673787"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="P5313_02220"
     CDS             <1..466
                     /locus_tag="P5313_02220"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HRY79211.1"
                     /translation="CGPATPKSRVPADWTSWRKPVDRVLNYTIPGHGDKARVIYVNAP
                     GWEYAGGAGSGRLEFPEGTVVVKEIYASGSPAPGEKPVTLDVMVKAPGDPDARGGWLW
                     LVKDMASGKETVITTNFCQACHANANSSHPYGDRNPGGAFRDYLYFPPIPGT"
     gene            502..2283
                     /gene="sulP"
                     /locus_tag="P5313_02225"
     CDS             502..2283
                     /gene="sulP"
                     /locus_tag="P5313_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HRY79212.1"
                     /translation="MSPSSFSSLLRGAFSPKLFHVLRSGYTREMFFKDLTAGLTVGVV
                     ALPLAMAFAIGAGASPAQGLYTAVIAGFLMAVLGGSRYQVSGPTGAFVVIIAGVIVRH
                     GMGGLVTATLLAGIILVILGVSGLGKLIKYIPYPVTTGFTTGIGLIIAGGQLKDFLGL
                     SVPDPHAEFFERIGEVIEYGSTLSPATVGVGAGTLTAIILIRKLAPRVPAAVTAVALG
                     AAAVWIFRIPVETVGSKYGELPGGFPGPILPDLRLATIRAVFPSALTIALLGAIESLL
                     SAVVADGMTGDRHNAGTELTAQGIGNIAVAFFGGIPATGAIARTATNIKNGARSPVSA
                     IIHSLVLLAFALFLSKAASAIPLAGLSAVLLVVAWDMSELPRFLGMRKAPKSDLAVML
                     TTFVLTVAIDLTVAVQVGLLLAVFLFLRRTSETSSVSSIREILESEDSDTPDDPEAMG
                     RKTVPAGCEVFEIEGPFFFGTADLLQDTLTDLEKPPRTFILRLRKAPAVDATGLNALA
                     SFHKYCRRHGTTLILSGVRDQPRKALESFGLVEAIGVENILPDIDAALARAASILAAA
                     KADSEAKAEAAAAERLPVGPKGIRLPK"
     gene            2374..2455
                     /locus_tag="P5313_02230"
     tRNA            2374..2455
                     /locus_tag="P5313_02230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2408..2410,aa:Leu,seq:gag)
     gene            2726..3718
                     /locus_tag="P5313_02235"
     CDS             2726..3718
                     /locus_tag="P5313_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRY79213.1"
                     /translation="MHDEGIGSVQENPRILEYCFSAWTPDSEYVKSRVRRGVSISAAY
                     IYQNAKLLKKHLGQALKGVRLQDLTAGRVERILMDLADRGVNPRTINTTLQALRVPIR
                     HFCKMNRIPDPLQYVQPLKEDPKTRGALSFEEVRALIALEDVSPRTRAAFLLAALCGL
                     RLGETCGLKWEDVNREAGILHVRGNYVKRDGLKKPKWSSFRDIPLPSVVLEALDLCGS
                     MPEAQGSPFILYNDRRKDVPAWPRTMTEGFSKMLDRIGLDEAARAERNISFHSLRHSF
                     VSMSRAAGLPDFVTQRLAGHRSAGMMERYSHAEVIDFAAARLALEGALKAAGRE"
     gene            3728..4279
                     /locus_tag="P5313_02240"
     CDS             3728..4279
                     /locus_tag="P5313_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79214.1"
                     /translation="MDSKISQFPELPPGFLADCRFLLENGRHNFETLEITARTLVEHI
                     EAKKAALLSDQKPSPRARILSFPVTSEAMRRTREHSLLDLISKGFNVWGYRWPTIKCP
                     SPVNSVYAMMEKDGVFYEYVYSYGDGSDPVNCISEIPKDRLLECYKRFGPRTGRLIPP
                     KMALRILTRASGCTALESEPGGT"
     gene            complement(4687..4890)
                     /locus_tag="P5313_02245"
     CDS             complement(4687..4890)
                     /locus_tag="P5313_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79215.1"
                     /translation="MPKLEIDFSIEAIQEEAGAKRRGYLLRLTESEHERLKAYAATVG
                     IPLSVILRSVAGKLLEAAPGVKS"
     gene            complement(4912..6006)
                     /locus_tag="P5313_02250"
     CDS             complement(4912..6006)
                     /locus_tag="P5313_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRY79216.1"
                     /translation="MTKLEQCRAELEEATIRGKRFLDEGNMPAAAIVAEEIRTKRAQL
                     DRELEVHRADESVARNLPAPSMPSSGGGWREIRQAMMEKRAITANGAGINTVSDVVSA
                     LVDGGKLRSKVSIFFGKNATSVVPVFAPSLALPVGTAPGATGTGSDTTAVLAGKNLVL
                     KPWYNTLAVGMDALISTNIEAALPGILAKNFAGAIDKGILVGAGSGSDMLGVFVADAG
                     GVPTGSDIACAAAGTPKWVDVAKLALTVNGLGNDPATTAIVMHPNIMAPLLAESTTGV
                     DPYKVEYLNKGTILGIPVILSSYCPTTLTAGSYVVVGGDFAHYGLAIAQELTIDPIKT
                     VGSDNVTFQSFMYMQGSPLIGSSFRRLKTV"
     gene            complement(6008..6574)
                     /locus_tag="P5313_02255"
     CDS             complement(6008..6574)
                     /locus_tag="P5313_02255"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HRY79217.1"
                     /translation="MAGMEKRTFHGEKLEIRKEGSKKFVEGVLRYQVLSEDLGGFREK
                     IAPSVFSRTLNAGSDVKMLWNHNDDEILGSTKAGTLVIENRAEGLHFSCLIPETASNR
                     FETIQRGDVDGVSFGFIPEAETWDHNTTPPTRTLTAARLIEISPTPFPAYPAFSTQAT
                     QRSRDAGESLRLRLEIENAIAMYGKERV"
     gene            complement(6955..7239)
                     /locus_tag="P5313_02260"
     CDS             complement(6955..7239)
                     /locus_tag="P5313_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79218.1"
                     /translation="MPDLAKTMAEIRRLIEEAKRLETLHRPDCNESVYWTGRMDGLAE
                     GLAALEKYENSPGRQDPELSDLLEEDEEPVLDDAAARAFAKALRAARGGL"
     gene            complement(7456..8256)
                     /locus_tag="P5313_02265"
     CDS             complement(7456..8256)
                     /locus_tag="P5313_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4373 domain-containing protein"
                     /protein_id="HRY79219.1"
                     /translation="MKNNIPYFQHRTDSHSDYRHKIILGLFGMEGIGAYWTLLEIIGN
                     APDCRIKYSDIGVQGSLAYELGMHKPQGRFQGFIDTLVQYELIVLSEGYLTSLELQET
                     LEYVSRKRIRNHQQYIQRKGSAVDSSDSTTKTDNSAAETDNSASPNHTIRTGTGTGTG
                     NKQERAARVELSPGEEEALRAFALGWAKKQRGIGNPEGFARKRWQDSDVVAAWRKSLE
                     PPPPPVEPIPPATPDEIAAANEEADRIMREAMARPGLGQALSGAVGAV"
     gene            complement(8253..8453)
                     /locus_tag="P5313_02270"
     CDS             complement(8253..8453)
                     /locus_tag="P5313_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79220.1"
                     /translation="MAAATESGAALLERLRPEIERALSSAPTFGYLGFRVHFNDGQPV
                     RIEYEGQVSRFIRDRPPGRGAT"
     gene            complement(9636..12671)
                     /locus_tag="P5313_02275"
     CDS             complement(9636..12671)
                     /locus_tag="P5313_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRY79221.1"
                     /translation="MSGPVILNLLGPPLVEVDARPIELTRRKTLALLARLAVEPGEHA
                     RDCIAALLWPECGQSRARGNLRSSVFELNHALGAGLLVTRQDLLRLDPEKIRVDVDAF
                     QSGAVPCESHDPDLLCPECAARAEAAASLWRAPFLSGFSLRDCPDFDEWQLAMSVRLR
                     GEYCVLLRRLTAYHRRRKDYRKAEAFALSWIREEPFEEEARLARVEILLAAGRRRAAL
                     ESIEEWERVSRTELGRPLSRRMGSRVRELREAESPDGTDSPTLAGIPEAAPGPLDAVG
                     GSGWMIGRDGELAELTSILRGGTCRLCSITGAGGIGKTLLARNVLRSAGRDFPEGSHF
                     VELAALREPSQVPDAVARELGILESRRSEKGVEEAILSAIGRSRRLLVFDNLEHLPGA
                     RGFVKRLLESCPNLGILATSRERLGIPGERECRLRTLAIPPRTRSATRADCEGYSSIR
                     LFSELARSANPDFALSDDNAFAVSSICRRLDGLPLALELAAARLAVLEPEELLSRLDR
                     RFDLLHAESSDLPERHRALLSAIDWSWDLLDGRDRGVFAALAVFPGGFDLAAAETVCE
                     APSDPDPGKLVDGLASLVGKSLVVRYVEGGATRFRMLESIREYAAERLAESPENRIVR
                     LRHAAHFLALAERETPALHGRRQSSALARLEREHLNLRTALEFFRASEDAEAALRLVS
                     SLEWYWHRSGRWNEGRASIKACMSLPRTDDFPLLRGRALRALGWLDFVQGEWREARGH
                     YLEAERLLRAEEDRIWLARCLSDLGVVECWLGDRAQGMERAREAIALARDLGDPGTVS
                     RALVWAYGTNGGRKAGEDQDERLEEAVRCARQAGDRWTEAHALESLGDSLREEGLFNM
                     AGACFEEGLQGFDELGDAWMKAWSLEGLGMNDCLKGDPARGFQHLRKAVRLFERSGAR
                     LDAAYVLGELGIAAGLLGDGEKSDLFLGASWALRDASVDPVAELPPGGGPQTQVPSPL
                     ERALASAAARKSDAWCRGIRLRFEEAVRLAEEDLGDRYP"
     gene            12862..13383
                     /locus_tag="P5313_02280"
     CDS             12862..13383
                     /locus_tag="P5313_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HRY79222.1"
                     /translation="MKKRFGSVILALAGIFLVAAAVAGREAPGVFVNDAEERPRRIPG
                     EAAVIRAIFETYAACIERHDADGWMALWDEEGVQLPPDGPMFVGKPSIKVGNYQEFKD
                     TSKKWTMNIQTQEVQVFPAEGYAFARGVYDWTCTPVGGGEVLCYDGKFMTIFRRQADG
                     TWLIYRDIFNSNL"
     gene            13561..14001
                     /locus_tag="P5313_02285"
     CDS             13561..14001
                     /locus_tag="P5313_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY79223.1"
                     /translation="MTQAAAAQLDATTDGTAILNLFDRYVDRIVNYDVDGWMALWDED
                     GVQLPPGSPMCVGKDEIRRGNQGWISDRSVIWTMHIDTQEVKVFPKEGYAFARGVYGW
                     TQTPRNGGTTSRYDGKFLTVFRRRNDSPWRVYRDCFNSNVPTVE"
     gene            14089..14844
                     /locus_tag="P5313_02290"
     CDS             14089..14844
                     /locus_tag="P5313_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY79224.1"
                     /translation="MSGILGSIAGGRVLDAATGGGGFALLLADRLAGWTEIVGIDTNP
                     KAAASFSAAAAGRPGIRFETRDARATGWPDASFDTVAVANSLHHFPDPSALLAELFRL
                     LAPGGTFILTEMHRDTRDPARKSHVLLHHWWAEVDRALGVEHRETYGRAELTAFAASL
                     GLEDLVLHDLEEDGDPRDSEALEEVGRAIDAYIARVKGTPDSERLIMRGEELRSRVDR
                     TGFLGAPGFLAVGRKRIAPHLPGVNSKSDTTTS"
     gene            complement(14799..15527)
                     /locus_tag="P5313_02295"
     CDS             complement(14799..15527)
                     /locus_tag="P5313_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79225.1"
                     /translation="MSGPVSGARLLRSAAAFLAALSAAAPASAAGPRILDFGGLSWTV
                     RRTAGPSAPGPNVFRDTQDQVRTDEAGRLVLSVRKREETWTACEIIARKATGYGTYRF
                     DLDGGARDLDPGIVFGFFTWDPNPGLHNREIDIELSRWGDPEGPDGWFTVQPYERPGN
                     QDSFRLPPASVYRFEMRWEPGSVSFACRADGREVRAWTFRGEVPDPGKARVRFNLWLF
                     RGAPPAGPGPYDVVVSDFEFTPGR"
     gene            complement(15524..16759)
                     /locus_tag="P5313_02300"
     CDS             complement(15524..16759)
                     /locus_tag="P5313_02300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY79226.1"
                     /translation="MSEARDADSPSPVAVRPAAGRRDLEAFLRFPWTVYAGDPLWVPP
                     LLPERREALDPRRGELFRRGGEAAPFLARRGDRIVGTVAAWRDPDADARSGRKTCFFG
                     FLEYRDDPDAARALLDAAEGWGRARGCVELYGPFHLDYENAYGVLVEGRDRPPALLCG
                     HSPPYYLPTMELLGFEPARGDNLAFEVRLDRSVPDRERLARLADWSRRRRGYANRGAD
                     FSRFEEEVDRVHDLLNRSLAHLPGFIGWERSAVRALLAPFRRFADPDLILFAEKDGKT
                     VGFFPAIPDLNEIFRTCDGFSDLSSTLAGLWRLAFRSRPLRPADRFPSTILGRRYRCV
                     TIKSVLVPPEHWNAGVAALLFDEMLRRLEGRGYEWIDLSLTSADNPNTPVLAERFGGR
                     VYKRYRVFRRPIGPAGAVS"
     gene            complement(16756..18120)
                     /locus_tag="P5313_02305"
     CDS             complement(16756..18120)
                     /locus_tag="P5313_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79227.1"
                     /translation="MNGKLGLGRKLAYGAGDLGFSVTTTMAGAYLSVFLTDVVGLAPA
                     LAAAAIFVGRSVDYLNDPLIGHLVDRTRSRWGRRRPYILFGALPFGAAFLLLWTRPAL
                     PSPVLLAAYYGAAYALFDVAATFVYMPYYALTPEIAPSYDERTSLTSWRMAFSILGSL
                     LVFTVPPALAGGFGPDSARRVFLLACGAAVVSVAPLFSCFAAVRERPEAPTEQRPGIR
                     DSLAAAFRNRAFLLGAGIFLATWVCLDLLQALIVYYIKHVVRREASMDLIMGLIFVTA
                     LVSLPLWNFVSARLDKRRTYAVGAGLLAVCLGALAFLDPSTPLAFIAALSVAAGVGVG
                     AAHVLPWAIIPDAVELDEATTGARHEGMFYSLITLAQKVASSLALPLVLLVLGAAGYV
                     PASSDQPERAVAGIRLVMGLGPAILLGAGAVFASVYPLTREAHARLRAELAARAAPES
                     GGEP"
     gene            complement(18197..19795)
                     /locus_tag="P5313_02310"
     CDS             complement(18197..19795)
                     /locus_tag="P5313_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY79228.1"
                     /translation="MGSTCPPRRRSAAAFLAFIAFALSAGPLAPAPRTVRIGLFPAAP
                     LVFEKDGKPAGLFIELAEHFARELGWNLEFERGTWENLQAELTDGRIDLLPALGYTPE
                     RTKTRDFSKHPVFVDSGVVFTSPRFPIHTVFDLEGRKIAAVRGSVFTEGFLIYTGSFG
                     ISCRMVLTEDNAQVMAAIADGSADAGVTIYSLGTELARNYPVRVTPISFSPVALHFAV
                     RKDANADILEGIDRLMAAMVNDPESFYSRAFRKWTQPEPERTVPTWIWFTLAGIAFAG
                     MLFAAWSLSLRSQVAAKTKSLTAEIQERIRAEERLKATLLQEETLLRELAHRIGNSLQ
                     LVKSLVGLKAADYPDKSVEAFAQNLGGKLDSIILAQRMLPESDDLSSIPLRKYIENLT
                     ETILSELGRSDGSIRVRIEVDAPDILLDIAIPLGLIVNELVSNSLLHGIPEGREGTIR
                     IEACETAPGRLCVRYADDGSGVPPGFDPRTQDSSGFRFILALAEGQLKGAVTFPAGNG
                     FRCSFEVPTDLYHRRITLRSDCSE"
     gene            complement(19839..20522)
                     /locus_tag="P5313_02315"
     CDS             complement(19839..20522)
                     /locus_tag="P5313_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY79229.1"
                     /translation="MEETGGYAARWGLDRILPRDLYESLRPVRHGPGHPIVRSGDPAR
                     DLLFFVEGRAKAYSILENGRGILAAFYRPPEVLGELELFTSDTYALTVEALTDTVCLA
                     LPSATVRASVDRYSRLLAFLCERLGRKLGERAQAESVNLRYPVEARLASYLLAASDPE
                     GRIVGTDDLGELADFIGASYRQLARAVRKFRDLGILDGARGRIRILDRARLEPRAGDR
                     YDREGGGRT"
     gene            20655..21536
                     /locus_tag="P5313_02320"
     CDS             20655..21536
                     /locus_tag="P5313_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY79230.1"
                     /translation="MMYTILASLTGVLITLMNSVNSRLAATAGYPAAAVTAHVVGLAA
                     VTILTLARKEPPEPGRVPFRFYLGGFFGVGTIFACNFAFSTLSASLAVALSLLGQAGF
                     SLLADGTGLLGRRKYPLGPGRLPGIALAAAGTAVLGWGGELRWAAVPAALAAGILPGI
                     SFILNSELGRRRGVLRSARWNYLTGLAGTAAVAALTGLPAAPAARAVLEAGPLLALGG
                     GLAGVAVVATMNFVFPRVPAFTATILLFSGQALAGVALDWAARGELDLHKLIGTGIVL
                     AGLVLDSRISRRPAAAC"
     gene            21547..22233
                     /locus_tag="P5313_02325"
     CDS             21547..22233
                     /locus_tag="P5313_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRY79231.1"
                     /translation="MPRRYDTVFIDADDTLFDYPRAEERAFSRTCVRFGLSDTPELRA
                     AYRRINREQWKLLERGGTDQATLRVERFRLLFEETGDRLDPAAVGGAYVEELSQGADL
                     LPGAREICGYLAERYLLAIVTNGLADVQRSRLALSGLGDLVAALVISEEAGTSKPDPR
                     IFEYACGRVGRTDRGRMLMVGDSLESDIRGGTAFGIDTCWYNPRGMENPTELRPTYEI
                     RYLAELRSIL"
     gene            22403..23287
                     /locus_tag="P5313_02330"
     CDS             22403..23287
                     /locus_tag="P5313_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY79232.1"
                     /translation="MKNRGGGSAFKIAAFLVPVGVLFAAFFLYPFVFTLFASLTRWRG
                     IGAMRFIGLGNFGRLLGDPTFRLAIRNNVTWALAAGFVQVPLAALVAILLARRPRGWK
                     FLRTVYFLPNVISTVALAMVWSALYNPQYGFFNGLLKLAGFEARNFLGDPSTALLSVI
                     VSSVLYIGYFMIILLAAAMNIPQELYEAAKIDGANAFQQEFLITVPMLRGTLVTSITL
                     AMAYGMRHFEATFLMTGGGPAYSTTTMGIDLFLKMDALRYSEASAAGVILILLGTVVI
                     VAIRKILGNKDPMSEAGQ"
     gene            23284..24141
                     /locus_tag="P5313_02335"
     CDS             23284..24141
                     /locus_tag="P5313_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY79233.1"
                     /translation="MSGTPGSGGLRAGAALAVLLKWIVLVFFLAVAVLPLAWLVLNSF
                     KTNLEIQSSPFSLPARPDFENYANAVALADLPRLFLNSVIVAVGTVFLNLVASSMAGF
                     VLARESWKGRETVRTVLEAGVLVPILAFMVPYFTLVTRLGLYDKLVTLVLVYAAINVP
                     VSIFLVTSFMRAIPKEMEEAAIIDGCSFVQRFTKVVLPLSRSGLVTAGTFCFIYAWNE
                     FLMAMLLTSSISARTIQLGIRFFTSQFITDYAGMFAAIVLSILPSVLAYVFLHNKIIA
                     GLTAGAVKG"
     gene            24200..25228
                     /locus_tag="P5313_02340"
     CDS             24200..25228
                     /locus_tag="P5313_02340"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRY79234.1"
                     /translation="MDFERKRFGTLSTGEEVDLYILKSADLSLGITPYGATLVSLVVP
                     GRSGRDDVLLGFSTLGPYTRKHPFFGSTVGRFANRIAGGRFGLGGKDYVLARNNGPNS
                     LHGGLRGFDKHVWKAEAYREDGGVFVRLSHTSLDGDEGYPGTLRAAVTYGLSGDNEVS
                     ALYEAEVDAPCPVNLTNHAYFNLAGEGRGTILDHEAALFASAYLPVDGTLIPTGELAP
                     VAGGPFDFRTPKPIGRDLEAAGGGYDHCFVVDGVPGNLRPCARVGDPGSGRTLEVSTT
                     QPGVQFYTGNFLDGIPGKRGALYGKHAGFCLETQGFPDAPNQTTFPDCVFGPVRPYRE
                     RTVFTFGY"
     gene            complement(25308..26141)
                     /locus_tag="P5313_02345"
     CDS             complement(25308..26141)
                     /locus_tag="P5313_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY79235.1"
                     /translation="MRLATRKKIITAAVAVFALAVLAVELYPIAITLLNGFRRDIVIL
                     SGQKLSLKQFTGASYALVFQNGQLMRAMRNSVIVGLLSTALSVGVGAMAAYGLTRFKF
                     RGKNALAYSFLVFRMLPQMSLVVSLYLMFRTVGIRDTIFGITLAHSSFNVPYVIWMLI
                     PFFTVVDKSYEEAAMVDGCSRWGMFRLIFLPLAAPGLVVAAVFAFINSWNEFLYALIL
                     TGVNARTAPVAISSLIGGDTLTWGQMCAASTIMLIPVFVFTLGMQKFLIRGVAAGGVK
                     G"
     gene            complement(26138..26977)
                     /locus_tag="P5313_02350"
     CDS             complement(26138..26977)
                     /locus_tag="P5313_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY79236.1"
                     /translation="MFAVLIFPIVFSFVVSLFDWPLSAGGGTRRFVGAGNYVELVRDK
                     EFWNSLRLQLGFIFIAIPIEIALGLGAALLLNREFRGGRVVRALILLPVFFLPIVSGM
                     TWRFMLQPRYGALNNFLLSLGAPEITWLGHPTYAYAAVIVQDVWRMWPFVFMLLYAGL
                     VGIPADILEAAEIDGATSTKRLTKIILPLLYPTILTALLLRIIDALRIFSEVYVMTEG
                     GPGMATTLFSLYTHRQAFGYMKVGMSSAMAIFLLVISILFAVTLVRKNMSLEKLEEQG
                     ENR"
     gene            complement(27060..28349)
                     /locus_tag="P5313_02355"
     CDS             complement(27060..28349)
                     /locus_tag="P5313_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY79237.1"
                     /translation="MNLKKSVLLGALLAAAAFASAQQKVINVLLWDDPYPKALVAMLP
                     EFEKATNIKVNIEMLQPPQVLTKTAVAVTATSTDYDVVGIDEGNVPVFASLLQPFAQW
                     PAGKVYPKTDPKSVTPKMLSIGTWNSVQLGIPINGNLYVWMTRKDLIENPTYKTQFKA
                     KYGYDLGIPKTFKELLEMGTFFHENGIAAGFGPFNGGPAGVLSEAVFMWESHGTKFID
                     YTGGTFKVVLDEAKAIEGMEFYKKLMAISPKGAETMAHVERQAAFAADPKGVFSMFIW
                     PAQIAAYEDPNKSLVAGKIAYSAPPAGPRGQAAVTGAWAICIPKASKNQDAAAEFVYW
                     WSSKAVADKLVQANTIPARTESLTNPAFVSSKPWLPALAASMNVAASRPRFKEIGQVQ
                     DVVKKYWIMGITGQMPTKDAVQAIVRETNDILKKAGY"
     gene            28661..30724
                     /locus_tag="P5313_02360"
     CDS             28661..30724
                     /locus_tag="P5313_02360"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRY79238.1"
                     /translation="MSRLEIIRTGNGFEVRLGGRLAIEHGPGRPFLSAGRGEGRYDMH
                     HGNFEVTESLAELVALEDFELAPRPDGGAEVSFSRRGLYPVRADFSEVRGRLVVSFRA
                     EPGEGGSAPPNRYRVRLPAEPAEHVYGAGEQFSHFDLRGRVFPLWTSEQGVGRNKLTE
                     VTRLADLHDKAGGDYWWTFYPQPTFCSSRRIFYHFDATAWASFDFRFPDRHEYYVWGP
                     PASLTVGAADSMLDLVSDLTDLLGRQPELPDWAHDGVVLGIQGGTETCLAKLARARAA
                     GVPVSGIWAQDWEGINMTSFGQRLRWNWVWDPERYPGLDEAIRSLRAEGVRFLGYINP
                     YVAAGKSLFREAAAKDLLARRADGSVYLVDFGEFDAGIVDFTKPEAFAWYKGVIRKNL
                     VEFGLAGWMADFGEYLPTDAVLADGTPAELAHNEWPALWARANREAVDEAGASGEIVY
                     FMRAGWTGSQRWCPMMWAGDQNVDWSRDDGLPSVIPAALSLAMSGHGLHHSDIGGYTT
                     LFILKRTKELFQRWAELAAFTPFMRSHEGNRPGDNWQFDSDEETLRHLASMTRLHVAL
                     KSYLKDCVRQNALRGHPVQRPLFLHFEEDPEAWGIQDQFLLGPDLLAAPVLEEGARKR
                     RLHLPPGEWADFWSDRVVRSPSPSGTTLEVEAPIGRPPAFFKASSAWKDVFRKAADAA
                     SGAGR"
     gene            30721..31920
                     /locus_tag="P5313_02365"
     CDS             30721..31920
                     /locus_tag="P5313_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY79239.1"
                     /translation="MNHFRRDAPGSGDPLRSSDVRRRNENLVLRLIRASGDRGLSQSE
                     AVTATGLRAPTMLRIFSSLEESGYIESLPPAATEEEGKERKGRPPVSFAARADALYTL
                     GVEFWVDRVSLGLFDFRGNHLSSSVLALGGGVDADSVTEVIAGGVRAAMSARGLGGER
                     ILGLGLGAPGQVDVRSRRITFYSRIPGMRDYPMAEILERKLGIPVHLHNNCAVIALSE
                     YRYGNVGKTDSLFMFLLRSGVNGAFIDHGRIHLTSDGTTLESGHVPIDYRGKPCVCGA
                     RGCLEPYLADLAKEDLAAGRYLFEGLGPALEAGDPKARKVLRQAADYLASAVRSVRRL
                     LNPRAFLFVTASAPVSEALARLVPESLAESPSGFDDPPAAFVGTVYDPGLVQRGAADL
                     VLDAFLG"
     gene            complement(31927..33183)
                     /locus_tag="P5313_02370"
     CDS             complement(31927..33183)
                     /locus_tag="P5313_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY79240.1"
                     /translation="MTPRGLSATDIRRRNRSGILRLIHRHGALARNDLAGALGLTRAS
                     VTILSGELLDEGLLEEAGSSGPGGKAGRRKVFLRIRPGAGKLIGAGVEPDRLQVLVTD
                     LAGRVLGVRELPPGDFAGPGAGGHPGPDSREGSRKAEPGARLAEAVLGSASDILGEAG
                     PAAAGVVGAGLGVTGRVDPGTGTCLREPRIWDVPVSLAAPLAAALGVPVSVDNNVRAL
                     ALAELLLTEARRDPPPGLLFVKYGPGVGGAWTAGGPPWAGAQNRAGEIGHTLVEADGP
                     VCPMCGRRGCLESLVSLGALRARFGRGDRGRGNGIETLLDRLDREDPAGYSLLAARFA
                     RALGNAIELLDPSRVALYGLPFRDEALLGQIASRVESTGRPCEIRRSGLDPALPALGG
                     AALALDRFLEAGGELGVTKIAASGGR"
     gene            33315..34070
                     /locus_tag="P5313_02375"
     CDS             33315..34070
                     /locus_tag="P5313_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRY79241.1"
                     /translation="MATKDGKLFHARDSRAVLREIFNQRDSNLEFLSLAELDIADARS
                     TSLCHKREETLAFNLAGPASVFVDGTEYRMEHYDVLYIPKGAEYFVEAGAEPAHLYLY
                     RALAENVHPVQLRRWKDLKDDPSRIRLLTRKKVHLMFDVAERADKFMAGYTFYEDRSR
                     AWPPHNHTDQEECYSFIEGSGAMQVYEDDEKLTFVKGVEVGSHVTIPLLNYHPVFSHE
                     EPLCFIWCIAGERYWVGDKNKSFMDGTAKKVTT"
     gene            34067..34969
                     /locus_tag="P5313_02380"
     CDS             34067..34969
                     /locus_tag="P5313_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY79242.1"
                     /translation="MINPSPDRPALPAELADLAVRARSIRRACLESIAALGVGHVGGS
                     MSVVEALAVLYFRHMRLDPGDAKSPDRDRFVLSKGHAGPALYAALAEKGYLDRPLLLT
                     LNKGGTSLPSHADRQRTPGVDMSTGSLGQGFSAACGIALGLRMDGPRADGTVRRCWTV
                     IGDGESDEGQVWEAAAFAAHYRLSNLVAFTDANRFQIDGATEAVMHLRDLSAKWSAFG
                     WRTFRVDGHDLGALDRTILSACSPDPEADGEGRGRPAMIVLDTVKAKGVPALEGKAES
                     HNAPFGPADLEAALDAMDKEAPRG"
     gene            34962..35906
                     /locus_tag="P5313_02385"
     CDS             34962..35906
                     /locus_tag="P5313_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY79243.1"
                     /translation="MADREMRAVYAQTLTALAASDRRICVVEADLARATGTGPFAKAY
                     PDRFFNVGVAEANLVGVASGLSAAGKRPFAATFACFASRRAYDQFFLSANYAGLEVVL
                     VGTDPGVTAAFNGGTHMPLEDLALMRAVPGLAIVEPADPVSLEAAVKLALERKGCTYL
                     RLQRKPAPEIYGAGEAFEFGKAKLLRPGKDVVLAALGALMVGEARKAADSLAAEGIDA
                     AVVDVFTLAPLDRAVLLDLARKTGRIVTCENHRVTGGLGSAVAELLAEEGVPARLARI
                     GAGADEFGEVGTQDWLAERFRLTAPHIAAAARRLVKGV"
     gene            35909..36397
                     /locus_tag="P5313_02390"
     CDS             35909..36397
                     /locus_tag="P5313_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRY79244.1"
                     /translation="MEKKIRLALASDLSGFPLAREIVKHLNENHPEIEVIDFGIPSAD
                     QPQPYFIQAPKVAKAVQKGEADKGVLICGTGQGMAIVANKHKGVYACVVDDIFSAERS
                     KIVNNANVITLGGWITAPFLGCQIVDAWLAMAFTQKMEFKKDFLTNAFNQVVALEAEN
                     FK"
BASE COUNT         5928 a        12126 c        12374 g         6094 t
ORIGIN      
        1 ctgcgggccc gcgaccccga agtcccgggt acccgcggat tggacctcct ggcgcaagcc
       61 cgtggaccgg gttctgaact acacgatccc gggacacggg gacaaggccc gggtgatcta
      121 cgtgaacgcc ccgggctggg agtacgcggg cggggcgggt tcaggccgcc tcgagttccc
      181 cgaaggcacc gtggtggtca aggagatcta cgcctccggc agccccgccc ccggggaaaa
      241 acccgtgacc ctggacgtga tggtcaaggc tcccggggat cccgacgccc ggggggggtg
      301 gctctggttg gtgaaggaca tggcttccgg gaaggaaacc gtgatcacga cgaacttctg
      361 ccaggcctgc cacgccaacg ccaactcctc ccacccctac ggagacagga accccggggg
      421 cgcgttccgg gattacctct attttccgcc gataccgggg acttgacccc ggtagcccgg
      481 cgggggtaca gctaagggat catgagtcca tcatccttct catccctcct gcggggggcc
      541 ttctccccga agcttttcca tgtgcttaga tccgggtaca cccgggagat gttcttcaag
      601 gacctgaccg ccggcctgac cgtgggcgtg gtggccctgc ccctggccat ggccttcgcc
      661 atcggggccg gggccagccc cgcccagggc ctgtacaccg ccgtcatcgc cggcttcctc
      721 atggccgtgt tgggcggttc ccgctaccag gtctccggtc cgaccggagc cttcgtggtc
      781 atcatcgcgg gggtcatcgt gcggcacggg atgggtggcc ttgtcacggc gaccctcctg
      841 gccgggatca tcttggtgat cctgggggtg tccgggctcg ggaagctcat caaatatatc
      901 ccttatcccg tgaccacggg attcaccacc ggtatcgggc tcatcatagc cggaggccag
      961 ctcaaggatt tcctgggact ttccgtgccg gacccccacg cggagttttt cgagcggatc
     1021 ggggaggtga tcgagtacgg ctccaccctg tcccccgcca ccgtgggcgt gggggccgga
     1081 accctcacgg ccatcatcct gatccggaag ctggcacccc gggtgcccgc ggcggtgacc
     1141 gccgtggccc tgggggcggc ggcggtctgg atcttccgga tccccgtcga gaccgtcggc
     1201 tccaagtacg gggagctccc ggggggcttc cccggcccga tcctgccgga cctccggctg
     1261 gccaccatcc gggcggtctt ccccagcgcc ctgaccatcg ccctcctggg ggccatcgag
     1321 tccctgctgt ccgcggtggt cgcggacggc atgaccgggg accggcacaa cgccggcacc
     1381 gaattgaccg cccaggggat cggcaacatc gccgtggcct tcttcggcgg catccccgcc
     1441 accggggcca tcgcccggac cgccacgaac atcaagaacg gggcccggtc cccggtctcc
     1501 gcgatcatcc actccctggt cctcctggcc ttcgccctgt tcctatcgaa ggccgcctcg
     1561 gccatacccc tggcgggcct ttccgcggtc cttctggtgg tggcctggga catgagcgag
     1621 ctgccccggt tcctggggat gcgcaaggcc ccgaagagcg acctggcggt catgctgacc
     1681 accttcgtcc tgaccgtggc catcgacctg accgtggccg tccaggtggg tctgctcctg
     1741 gcggtcttcc tcttcctgcg ccgcacctcg gagacctcca gcgtcagctc catccgggag
     1801 atcctggagt ccgaggattc cgacaccccc gacgatcccg aggccatggg gcgcaagacc
     1861 gtgccggcgg gctgtgaggt gttcgagatc gagggaccct tcttcttcgg gaccgcggac
     1921 ctgctccagg acaccctgac ggacctggaa aagcccccca ggaccttcat actccgactg
     1981 cgcaaggcgc ccgccgtgga cgccacgggt ctcaacgccc tcgcgtcctt ccacaagtac
     2041 tgccgcaggc acgggacgac cctcatcctg tccggggttc gggaccagcc ccggaaagcc
     2101 ctggagtcct tcggcctggt ggaggcgatc ggggtcgaaa acatcctgcc ggacatcgac
     2161 gccgccctgg ctcgggcggc gagcatcctg gccgccgcca aggcggacag cgaggcgaaa
     2221 gccgaagccg cggccgcgga gaggcttccg gtggggccga aggggatcag attgcccaaa
     2281 tagaaggcag atttcaatgt cttcgcgtca tttgggactc catccggcgc ttgacgttcg
     2341 cggccccgac ggggtataaa agctccgttc cgcgcggcga tggtggaact ggtagacacg
     2401 ccagcttgag gtgctggtgc cgagaggcat ggaggttcaa gtcctcttcg ccgcatctgt
     2461 tttaacggta agtaattacc gaggcccacc gaacaacgga ggccgcccaa aatcgacctt
     2521 gttaccgcgt ttgttactgc gggaaggggg ttgtatggcg cggactccgt tttctgttta
     2581 taaccgcccc ggaaccgatg gaaagaatcg gttctatgcg cggttcctgg atccggatac
     2641 gggggcggtc ctgaaaaccg ttgcccttta tgacaaggga gccccggtcc gggggagggc
     2701 gaaggctctc caggaagccg cccggatgca cgatgaggga atcggctccg tccaggagaa
     2761 tccgcggatc ctggagtatt gcttttccgc ctggacgccg gactccgaat acgtaaaaag
     2821 ccgggtccgg cggggggtct ctatctccgc ggcgtacatc taccagaacg cgaagctctt
     2881 gaaaaagcac ctcgggcagg ccttgaaggg ggtccgcctc caggacctca cggcggggcg
     2941 ggtggagcgg attctcatgg accttgcgga tcgaggtgtc aatccgcgga cgatcaatac
     3001 cacgctccaa gccctccgcg tcccgatccg gcatttctgc aagatgaacc gcatcccgga
     3061 tcccctccag tacgtccagc ccttgaagga agacccgaag acccgcggcg ccctgtcctt
     3121 tgaagaggtc cgcgccctga tcgccttgga ggacgtttcg ccgcggaccc gggcggcctt
     3181 cctcctggcg gccctctgcg gcctccggtt gggggagacc tgcggcttga agtgggagga
     3241 cgtgaaccgg gaggccggga tcctccacgt tcgcgggaat tacgtgaagc gggacggcct
     3301 caagaaacct aagtggtcat cgttccggga tatccccctt ccgtccgtcg tcctggaggc
     3361 cctggacctc tgcgggagta tgcccgaggc ccaggggtcc cccttcatcc tgtacaacga
     3421 tcgccggaag gacgtcccgg cctggccgcg gaccatgacc gagggattct ccaaaatgct
     3481 cgataggatc gggctcgatg aggcggcccg ggcggaacgg aacatttcgt ttcattccct
     3541 ccgccatagt ttcgtatcca tgtcccgagc cgcggggctc ccggatttcg tgacccaacg
     3601 cctggccggg caccggagcg ccgggatgat ggagcggtat tcccatgccg aggtgattga
     3661 cttcgccgcg gcgcgcctgg cccttgaggg cgcgctcaag gccgcggggc gagagtagga
     3721 ggtaatcatg gattccaaga tttcgcagtt tcccgaactt cctcccggct tcctggcgga
     3781 ttgccggttc ctcctggaga atggccgaca taacttcgag acgcttgaga ttacggcccg
     3841 gactctggtt gagcatatcg aggcgaagaa agcggcctta ctttctgacc agaaaccaag
     3901 cccgcgggct cgaatcctgt cgttccccgt gacgtcggaa gctatgcgaa gaacgagaga
     3961 acattctctc ctagacctca tcagcaaggg ttttaacgta tgggggtatc gatggccgac
     4021 aattaaatgc ccctccccgg ttaattccgt gtacgcgatg atggagaaag acggggtttt
     4081 ctatgagtac gtctattcgt acggggatgg ttcggatccg gtgaattgca tttcggaaat
     4141 ccccaaggat cgcttgcttg aatgttataa acgcttcggc ccccggactg ggcgtttaat
     4201 ccccccgaag atggccctcc ggattctcac cagagcttca ggctgtacag ccttggagag
     4261 cgaacccggg gggacgtgat ttcatacccc ggaggcccgc cggacgccct ggcgggcctt
     4321 tcttgcaaat tccgcaggtt gcggatttga cgccggggcg gggaacctcg aaaacgtttt
     4381 ctaggtcgag aacggggcaa ctcccgcaac gttgcgggac ctccacaacg ttgcggaggt
     4441 ccccccgtga ttaactccgt caacgttggc ggaggtggac aacgttgtcc aggtcttcgg
     4501 gcaagtgggc ctctgcaaaa tcaaatcaag tttgaatttg caaaacggcc agcgtggccg
     4561 atttgtctgt ttgcaaattg ccaacagtta gcaatttgca tcggatgaaa aaggggaggc
     4621 cgtgctccga ctcccgtcgg tgccgtgcct ccccggatcc gcggccctca tagtcgcgga
     4681 cgctctctag gacttgaccc ccggggccgc ctccaggagc ttgcccgcga cgctccgcag
     4741 gatgaccgaa agggggatcc ccacggtagc cgcgtaggcc ttgaggcgtt catgctccga
     4801 ttcggtgagg cggagcaagt acccgcggcg cttcgctccc gcctcttcct ggatcgcctc
     4861 gatcgaaaaa tcgatttcaa gtttcggcat gattccccct cccggttccg cttagaccgt
     4921 cttcaggcgg cggaaactgg aaccgatcaa cggggacccc tgcatgtaca tgaaggactg
     4981 gaaggtcacg ttgtcggacc ccaccgtctt gatcgggtcg atggtcaact cctgggcgat
     5041 ggccaggccg tagtgggcga agtccccgcc taccaccacg tacgaccccg cggtgagggt
     5101 ggtcgggcag tagctggaca ggatgaccgg gatccccagg atggtcccct tgttcaggta
     5161 ctccaccttg tacggatcca cgcccgtggt ggactcggcc aggagggggg ccatgatgtt
     5221 cggatgcatc acgatggccg tggtggccgg atcgttcccc aggccgttca cggtcagggc
     5281 cagcttggcc acgtccaccc acttcggcgt ccccgcggcg gcgcacgcga tatccgaccc
     5341 ggtcggcacc ccgccggcgt cggccacgaa cacgcccagc atgtcggacc cggaacccgc
     5401 gccgactagg atccccttgt cgatggcccc ggcgaagttc ttggccagga tcccggggag
     5461 ggccgcctcg atgttggtcg agatcagggc gtccattccg acggccaggg tgttgtacca
     5521 gggcttgagg accaggtttt tcccggccag gaccgccgtg gtatcggagc ccgtgccggt
     5581 cgcgcccggg gcggttccga cggggagcgc cagggacggg gcgaacacgg ggaccacgga
     5641 ggtcgcgttc ttcccgaaga agatcgagac cttggagcgg agcttgccgc cgtccaccag
     5701 ggcggaaacc acgtcggaca cggtgttgat cccggccccg ttcgcggtga tggcgcgttt
     5761 ctccatcatc gcctgccgga tctcccgcca gccgcccccg ctggacggca tgctcggcgc
     5821 ggggaggttc ctggcgacgg actcgtcggc ccggtggacc tcgagctccc ggtccagctg
     5881 ggcgcgcttg gtccggatct cttcggcgac gatcgccgcg gcgggcatgt tcccctcgtc
     5941 gaggaatcgc tttccccgga tggtggcctc ttcgagttcg gccctgcact gttcgagttt
     6001 ggtcatgtta aaccctttcc ttgccgtaca tggcaattgc gttttcgatc tcgagacgga
     6061 ggcggagaga ttccccggcg tccctggagc gctgcgtagc ctgggtggag aaagccggat
     6121 acgccggaaa gggcgtgggg cttatctcga taaggcgggc cgcggtcagg gttcgggtcg
     6181 ggggcgtcgt gttgtgatcc caagtttccg cctccgggat gaacccgaag gaaaccccat
     6241 cgacgtcccc gcgctggatc gtctcgaatc ggttcgaggc cgtttccggg atcaggcaag
     6301 agaagtggag cccttcggcc cgattctcaa taaccagagt accggctttc gtcgaaccta
     6361 ggatttcatc gtcgttatgg ttccagagca ttttgacatc ggatccggcg ttcagcgttc
     6421 gggagaacac cgacggggcg atcttctccc ggaagccgcc aagatcctcg gagagcactt
     6481 gataccggag gacgccctca acgaatttct tcgagccctc tttccggatt tccagttttt
     6541 ccccgtgaaa cgttcgcttc tccatgcctg ccatggtgat tccttccggg tctcttttct
     6601 tcgggagacc cctccaaagg cccacgatcg gacgtgtatc acgttcgata tcaaaacttc
     6661 aagcgatttt ttcgctttcg cacgtcttcc ggattttgcg gttttgccat tttgtgagga
     6721 aaaagggacg gctgcggttc atggtttggc atcctgtttt cccggagggg ggtaggggtt
     6781 cgcctccccc cgagcctcgc gcgcgtgcgg aaggtgcgac aaaacggcaa gtgttgccga
     6841 tttgcaagcc ccgagcctcg cgcgcctgcg ggaggtgcga caaaacggcc agcgtggccg
     6901 atttgcagga agcctccccc gagccccgcg agcgtgcggg aggtgcgggg gcggtcataa
     6961 tccaccccgg gcggctcgaa gagctttcgc aaaggcccgg gccgcggcgt catcgaggac
     7021 cggctcctcg tcctcctcca ggaggtccga gagttccgga tcctgccgcc ccggagagtt
     7081 ctcgtacttt tcgagggcgg ccaggccttc cgccaggccg tccatcctgc cggtccagta
     7141 gactgattcg ttgcagtccg gacggtgcaa cgtctccaac cgcttggcct cctcgatcaa
     7201 tctccgaatc tcggccattg ttttcgcaag atcgggcatt gtcgcctcct cgcctgggca
     7261 ccgccccgga tgacctggga taggtttccc aggtgcttcc tcccgcgtcc tccgcctcat
     7321 gcttcccgcg gctcgccgcc gtgccgttcg tgtctctctc cccgcatagc gcataccgcc
     7381 ccaccgcctg ggccgcgttg atccggagat ggatcggggc gtcatggcct gcgtcctgta
     7441 cattcggcgg gatggtcata cggcccccac cgccccggac aaggcttgcc cgaggcctgg
     7501 ccgggccatg gcttcccgca tgatccggtc cgcctcttca ttggccgccg cgatctcatc
     7561 cggtgtagcc ggtggaatcg gttcaacggg tggcggtggc ggttccagac ttttccgcca
     7621 tgcggctaca acgtccgaat cttgccatcg ctttcgggcg aatccctccg gattgccgat
     7681 cccgcgttgc ttctttgccc agcccaaggc aaaggcgcgg agggcttcct cttcgcctgg
     7741 ggataactcc acgcgcgcgg cgcgctcttg tttatttcca gttccagttc cagttccagt
     7801 tctaattgta tgatttgggc tggctgaatt atcagtttcg gcggctgaat tatcagtttt
     7861 agtcgtggaa tcgctagaat caactgccga acccttgcgt tggatatact gctgatggtt
     7921 ccggattctt ttccgggaaa cgtattcaag ggtttcctgt aattccaagg aggttagata
     7981 gccctcgctt aggactatca gctcgtattg gactagagta tcgatgaagc cttgaaaacg
     8041 tccctgcggt ttgtgcatcc ccagttcata ggcaaggctc ccctgtaccc caatgtctga
     8101 atattttatc cggcaatcag gagcgttccc aattatttcc aacaacgtcc aataagctcc
     8161 gatcccttcc atgccaaaaa ggcctagaat gattttgtgt cgataatccg aatgggaatc
     8221 ggttcgatgc tggaagtagg ggatattgtt tttcatgtcg ccccccgccc cggaggccgg
     8281 tcccggatga agcgggatac ctggccctcg tattcgattc ggacaggctg gccgtcgttg
     8341 aagtggaccc ggaatccgag atagccgaag gtgggggcgc tcgaaagggc gcgctcgatt
     8401 tcggggcgga gacgttccag taaggccgcc ccggattcgg tagcggcggc cattaccgga
     8461 ccaccttgtc aacggtccag aaagcaacag gtggattgtc tccgcttgcc cgggaccccg
     8521 gatcgagggc tttctcgatc aacgtatcga tgacggcttc ggggatcagg cggcgggctc
     8581 cgatccggac cgatggcaac gcgccggact tgatccagcg gtgaaccgtg gggatggagc
     8641 atcgaagggt ttccgcggtt tcttccacgg acaggaaacg ggaacgaggc atagtggcct
     8701 ccgaatatct cgcccgttcc ctgtactccc ggctgattag ggccgggaga ttcgccaagg
     8761 aaaagtgcga gtaatcagag gcccgcctca atcgccgatc atcaacgtat gttgggcttg
     8821 acctcgggaa tcaagccaac ggtttcagct tgaacgactc ccgggcgggt aggggctccg
     8881 aggagccgac tccatgccac atggttgaac taattcgcgc ggaccttgca caatggacaa
     8941 acagtccaaa tcatgcaccc tactcggtga acaccgttgc gtatcctttc gggacgtatt
     9001 catgatatac agacttatca gaaaaccgca aggggtattt tttcgggtgt ctagcttatt
     9061 cggctagtga ctgttaccag taaaaaacag gattccagcg cctcaaaaaa gacagcgcta
     9121 gctttttagg ttttttgcta gtgctattca gcctcaaatc tgttaccgga attgttacca
     9181 caagtagcag ttgacggaga aaaagtgagg ccttattata caagctatcc ggctatcatc
     9241 gttgatcgta tcaatcggtc ggataatctt ttacgccgta gtaacaagcg tgtttgatcg
     9301 tccctgattt tgggtgattt tggggccttg agggcttgag gtgctggtgc cgagaggcat
     9361 ggaggttcaa gtcctcttcg ccgcacgaaa aacgagaccc ggtcttctga ccgggtctcg
     9421 tttttataag aggcgaagag gacttgatcc gaagcaccga ccctgagcac gcgagtcggc
     9481 gagcgtgcga aggggcaccg ggcttggagc ccggtgaggt gctgaggcgg cccggagcga
     9541 gcgaccaggg atgggtgcga gcgtagggca agcctcttcg ccgcaggaaa acgagaagcc
     9601 cgaccgaaag gtcgggtttc tcgtttttag aagggttacg gatacctgtc cccgagatcc
     9661 tcttcggcca gccggaccgc ctcctcgaac cggagccgga tcccccggca ccaggcgtcg
     9721 gatttccggg ccgccgcgga ggccaaggcc cgttccaacg gcgacggaac ctgggtctgg
     9781 ggaccgccgc ccggggggag ctccgcgacg ggatcgacgg atgcgtcccg aagggcccag
     9841 gaggctccga ggaacaggtc ggatttctcg ccgtccccga ggagtcccgc cgcgataccg
     9901 agttccccca ggacgtaggc cgcgtccagc cgtgccccgg atcgttcgaa cagacgaacg
     9961 gctttccgca agtgctggaa accccgggcg gggtcgccct tgaggcagtc gttcataccg
    10021 agtccttcga gggaccaggc tttcatccag gcgtccccga gctcgtcgaa accctggagc
    10081 ccttcctcga agcaggcgcc cgccatgttg aagagcccct cctccctcag ggaatccccg
    10141 aggctttcca atgcatgggc ctcggtccat cgatcccccg cctgccgggc gcagcggaca
    10201 gcctcctcga ggcgttcgtc ctggtcttcc cccgccttgc ggcccccgtt ggttccgtag
    10261 gcccagacga gggcccggga tacggttccc ggatcgccca ggtcccgggc cagggcgatc
    10321 gcctcgcggg cccgttccat gccctgtgcg cggtcgccca gccagcattc caccacgccg
    10381 aggtcggaaa ggcacctcgc gagccaaatc cggtcctcct cggcccggag caggcgttcg
    10441 gcttccaggt agtgcccccg agcctcccgc cactccccct ggacgaagtc gagccagccc
    10501 agggcgcgca gggcccggcc ccgaaggagg gggaagtcgt cggtccgggg aagggacatg
    10561 cacgccttga tggatgcgcg gccctcgttc cagcgccccg aacggtgcca gtaccactcc
    10621 agggaggaga cgagccggag agcggcctcg gcgtcctccg atgcccggaa gaattccagc
    10681 gccgtccgga gattcaggtg ctctcgctcc aggcgggcca acgcggaaga ttgacgccgg
    10741 ccgtggagcg cgggcgtctc gcgctcggcc agggccagga aatgcgccgc gtgccgaagc
    10801 cgtacgatcc ggttctcggg cgattcggcc agacgttccg cggcgtactc ccggatggac
    10861 tctagcatcc gaaagcgggt agcgcctccc tcgacgtacc ggaccacgag gcttttcccc
    10921 accagggacg ccagaccgtc caccagctta ccgggatcgg gatcggaagg tgcctcgcag
    10981 accgtctcgg cggcggccag gtcgaatccc cccgggaaca cggccagcgc tgcgaacacc
    11041 ccgcggtcgc gtccgtccag gaggtcccac gaccagtcga ttgcggacag gagggcccga
    11101 tggcgttccg gcaggtcgga ggattccgca tgcagaaggt cgaagcggcg atccagccgg
    11161 gaaaggagtt cttccggttc caggacggcc aggcgggccg cggccagttc cagggccagg
    11221 gggagaccgt cgaggcgtcg gcagatggag gacaccgcga aggcgttgtc atcggagagg
    11281 gcgaagtccg gattcgcgga ccgcgcgagt tccgagaaca ggcggatcga ggagtatccc
    11341 tcgcagtcgg ctcgggtggc ggatcgcgtc ctcggaggga tggccagggt gcgcaggcgg
    11401 cattcccgct cccccgggat accgaggcgt tcccggctcg tcgcgaggat cccgaggttc
    11461 gggcaggact cgagcagccg tttcacgaat ccgcgggcgc ccggaagatg ctcgaggttg
    11521 tcgaacacca gcagccgacg ggatctcccg atcgccgaga ggatcgcctc ctccactccc
    11581 ttctcggaac gtcgggattc caggatcccg agctcccggg cgacggcgtc gggaacctgg
    11641 gagggctccc gaagcgcggc cagctccacg aaatgggatc cctccgggaa atcgcgcccc
    11701 gcggatcgga ggacgttccg cgccagcagg gtcttcccga tccctcccgc cccggtgatc
    11761 gagcacaggc ggcacgtccc gccgcggagg atagaggtca gttcggcgag ctctccgtcc
    11821 cgtccgatca tccacccgga gcccccgacg gcgtcgaggg gacccggcgc tgcctccggt
    11881 attcccgcga gtgtcgggga atccgtgccg tcgggcgatt cggcctcccg gagctcccgg
    11941 accctgcttc ccattcgacg ggaaaggggg cggcccagct ccgtccggga tacccgctcc
    12001 cactcctcga tgctctccag cgcggccctg cgtcggcccg cggccagcag gatctcgacg
    12061 cgggccagcc gggcctcctc ctcgaagggc tcttcgcgga tccaggataa cgcgaaggct
    12121 tccgccttcc ggtagtcctt ccggcggcgg tggtaggccg tcagcctccg cagcaggaca
    12181 cagtactccc cccggagccg tacgctcatg gcaagctgcc actcgtcgaa atcggggcag
    12241 tcccggaggc tgaagccgga caggaagggc gcgcgccaga gcgaggcggc cgcctccgcc
    12301 cgggcggcgc attccgggca gagcaggtcg ggatcgtggg attcgcaggg gaccgcgccg
    12361 ctttggaacg cgtcgacgtc gacccttatc ttctcggggt ccaaacgtag aagatcctga
    12421 cgagtgacca ggagtccggc gcccagggcg tggttcagct cgaagaccga ggatcgcagg
    12481 ttgcctcggg cccgggactg gccgcactcg ggccagagca gggccgcgat acagtcccgg
    12541 gcatgctctc cgggttccac ggcgagccgg gcgaggagcg ccagggtctt gcggcgcgtg
    12601 agctcgatcg gccgggcgtc cacctcgacg aggggagggc ccagcaggtt caggatcacc
    12661 gggccggaca tttcttctcc gttttggata tacgctggtg tatatccccg cgtaccactc
    12721 cagcatagac ctcctgctcc cggcgcgcaa cggttctgca acgcggatgt aggacgctca
    12781 gggcggagat cgcggccggc cgggccgcgg cttcccgccg ccgtcggcgg cgaacgatcg
    12841 tttcaaggac tggagaacac catgaagaaa aggtttggtt ccgtgatcct ggctttggcc
    12901 gggatctttc tggtcgcggc tgccgtcgcc ggcagggagg ccccgggcgt gttcgtgaac
    12961 gatgccgagg aaaggccccg caggatcccg ggcgaagccg ccgtaatccg tgccatcttc
    13021 gaaacgtacg ccgcctgtat cgaaaggcac gacgcggacg gctggatggc cctgtgggac
    13081 gaggagggcg tccagcttcc cccggacggt ccgatgtttg tcgggaagcc ctcgatcaag
    13141 gtcggaaatt accaggagtt caaggatacc tcgaaaaaat ggacgatgaa catccagacc
    13201 caggaagtcc aggttttccc ggcggaggga tacgcgtttg cccggggagt ctacgactgg
    13261 acctgcacgc ccgtgggagg aggagaggtc ctgtgctatg acggcaagtt catgacgatc
    13321 ttccgccggc aggccgacgg cacctggctc atctaccggg acatcttcaa ctcgaatctc
    13381 tgaccgtccg gtatcgcaga tccccccgcg accgaaggac gccctaggcg tccttcggtc
    13441 cggtccggac ggtatcctga ctttgccccc gcgagcagta tttttccctg caacgtttct
    13501 gcaacggtct gacaagaagc ttggattggg aagcccggta cagtctgtaa ggaggaaccg
    13561 atgacccaag cggctgcggc tcagctcgat gcgacgacgg acgggaccgc gatcttgaat
    13621 ctcttcgacc ggtacgtcga ccggatcgtc aactacgacg tggacggctg gatggccctc
    13681 tgggatgagg acggcgtcca gctgcctccc ggcagtccca tgtgcgtcgg gaaggacgag
    13741 attcggcggg gcaaccaggg ctggatctcg gaccggtcgg tgatctggac catgcatatc
    13801 gacacccagg aggtcaaggt gttcccgaag gagggatacg ccttcgcccg gggggtctac
    13861 ggctggaccc agacgccgcg gaacggcgga acgacatccc gatacgacgg caagttcctg
    13921 acggtgttcc gcagacggaa tgacagtccc tggcgcgtct accgggattg cttcaactcc
    13981 aacgttccga ccgtcgaata gagccgatcc ctcgccggct atacaggagg cttcgggtgc
    14041 cctatcctgg agcctccagt tcgatgccgg aggtcggggt tggatacaat gtcggggatt
    14101 ctaggatcca tcgccggagg acgtgtcctg gacgcggcca cgggaggcgg gggattcgcg
    14161 ctcctgctgg cggaccggct ggcgggctgg accgagatcg tggggatcga cacgaatccc
    14221 aaggcagcgg cgtccttctc ggcggctgcc gcgggccgcc ccggcatccg gttcgagacc
    14281 cgggacgccc gggccacggg atggccggac gcctcgttcg acaccgtcgc ggtggccaac
    14341 tccctgcacc acttcccgga tccgtccgcc ctcctggccg aacttttccg cctgctggcc
    14401 ccgggcggaa ccttcatact gaccgagatg catcgcgata cccgggaccc cgcccggaaa
    14461 agccacgtcc tcctgcacca ctggtgggcc gaggtcgacc gggccctcgg ggtggagcat
    14521 cgggagacct acggacgggc ggagctgacg gccttcgccg cttccctcgg actcgaggac
    14581 ctggtcctcc acgatctgga ggaggacggg gacccccggg actcggaagc cttggaggag
    14641 gtcggacggg cgatcgacgc gtacatcgcc cgggtgaagg ggacgccgga ctcggaacgc
    14701 ctgatcatgc ggggcgagga gctccggtcc cgggtcgacc ggacgggatt cctgggagcc
    14761 cccgggttcc tggccgtcgg gaggaagcgg atcgcccctc acctccccgg tgtgaactcg
    14821 aagtccgata cgacgacatc gtaggggccg gggccggccg ggggggcgcc gcggaagagc
    14881 cagaggttga agcggacccg ggccttcccg ggatcgggga cctccccccg gaaggtccag
    14941 gcccggactt cccggccgtc cgcccggcac gcgaaggaga ccgaacccgg ctcccagcgc
    15001 atctcgaagc ggtagaccga ggccggcggc aggcggaagg aatcctggtt ccccggccgt
    15061 tcgtagggct ggaccgtgaa ccacccgtcc ggcccctcgg gatcccccca gcgggagagc
    15121 tcgatgtcga tctcccggtt gtggagtccc gggttcggat cccaggtgaa gaagccgaac
    15181 acgatcccgg gatccaggtc ccgggctccc ccgtcgaggt cgaagcggta ggtgccgtag
    15241 ccggtcgcct tccgggcgat gatctcgcag gccgtccagg tttcctcccg cttgcggacc
    15301 gagaggacca ggcggccggc ctcgtccgtc cggacctggt cctgggtatc ccggaagacg
    15361 ttcggtcccg gggccgaggg tcccgccgta cggcggaccg tccaggaaag acccccgaag
    15421 tccaggatcc ggggtcccgc cgcggaagcg ggggccgcgg ccgagagggc cgccaggaag
    15481 gcggccgcgg accggaggag acgagcacca cttaccgggc cgctcacgag acagccccgg
    15541 caggccctat ggggcgtcgg aagacccggt accgcttgta cacccggccg ccgaagcgct
    15601 cggccagcac cggtgtgttc gggttgtccg cggaggtcag ggaaaggtct atccattcgt
    15661 agccccgtcc ctcgaggcgg cggagcatct cgtcgaagag gagggcggcg accccggcgt
    15721 tccagtgctc cggcggaacg aggacgcttt tgatggtcac gcagcggtag cggcggccca
    15781 gaattgtact cgggaagcgg tcggccgggc gcaggggccg tgaccggaag gccagccgcc
    15841 aaaggccggc cagggtcgag ctgaggtccg agaatccgtc gcaggtacgg aagatctcgt
    15901 tcaagtccgg tatggccggg aagaagccga cggtcttgcc gtccttctcc gcgaagagga
    15961 tgaggtcggg atccgcgaag cgccggaagg gggccaggag ggcccggacc gcggagcgct
    16021 cccagccgat gaatccgggc aggtgggcca ggcttcggtt gaggaggtcg tggacccggt
    16081 cgacctcctc ctcgaagcgg gaaaagtccg cgccccggtt ggcgtacccg cgccgccgcc
    16141 gggaccagtc ggccaggcgg gcaagccgtt cccgatccgg gacggacctg tccaggcgga
    16201 cctcgaaggc caggttgtcc ccccgggcgg gctcgaagcc caggagctcc atcgtgggca
    16261 ggtagtaggg cgggctgtgg ccgcacagga gggcgggggg ccggtcccgg ccctccacca
    16321 ggaccccgta ggcgttctcg tagtccaggt ggaaggggcc gtagagctcg acgcagcccc
    16381 gggcccggcc ccagccctcc gcggcgtcca ggagagcccg ggcggcgtcc gggtcgtccc
    16441 ggtactccag gaacccgaag aagcacgtct tgcggccgct ccgggcgtcg gcgtccggat
    16501 cccgccaggc cgccacggtc cccacgatcc ggtccccccg ccgggccagg aagggggccg
    16561 cctctccgcc ccggcggaag agctcgcccc ggcggggatc cagggcttcc cggcgttccg
    16621 gcaggagggg cggcacccag aggggatccc cggcgtagac ggtccagggg aagcggagaa
    16681 aggcctcgag gtcccgtcgg cccgcggcgg ggcggaccgc cacgggggac ggggagtccg
    16741 cgtcccgggc ttcgctcacg gttcgccgcc cgattccggg gcggcccggg ccgccagctc
    16801 ggcccggagc cgggcgtggg cttcccgggt gaggggatag acggaggcga agacggcacc
    16861 cgcccccagg aggatggcgg gccccaggcc catgaccagg cggattccgg ccaccgcccg
    16921 ttcaggctgg tccgaggagg cgggcacgta gccggcggcc cccaggacca ggaggaccag
    16981 ggggagggcc agggagctgg ccaccttctg ggccagagtg atcaggctgt agaacattcc
    17041 ctcgtggcgg gcccccgtgg tagcctcgtc cagctccacg gcgtccggga tgatggccca
    17101 ggggaggacg tgggccgccc ccaccccgac ccccgcggcc acggacaggg cggcgatgaa
    17161 ggccagcggc gtcgaggggt cgaggaaggc cagggcgccc aggcagaccg ccaggaggcc
    17221 cgccccgacg gcgtaggtcc tccgcttgtc caagcgcgcg gatacgaaat tccacagggg
    17281 cagggagacc agggcggtca cgaagatgag gcccatgatg aggtccatgg aggcttccct
    17341 gcggaccacg tgcttgatgt agtacacgat gagggcctgc aggaggtcca ggcagaccca
    17401 ggtggccagg aagatccccg cccccaggag gaaggcccgg ttccggaagg ccgcggccag
    17461 ggaatcccgg attccgggac gttgctccgt cggggcttcg ggccgctccc gcaccgcggc
    17521 gaagcaggag aagaggggtg ccacggagac gaccgccgcc ccgcaggcca ggaggaagac
    17581 ccgccgggcg gagtccggcc cgaagccccc cgccagggcc gggggcaccg tgaagaccag
    17641 gaggcttccc aggatcgaaa aggccatgcg ccaggaggtc agggaggtcc gctcgtcgta
    17701 ggagggggcg atctcggggg tcagggcgta gtagggcatg tacacgaagg tggcggcgac
    17761 gtcgaagagg gcgtaggccg ccccgtagta ggccgccagg aggacggggg aggggagggc
    17821 cggccgggtc cacaggagga ggaaggccgc cccgaagggc agggccccga aaaggatgta
    17881 gggcctcctc cggccccagc ggctccgggt tcggtcgacc aggtggccga tgagggggtc
    17941 gttcaggtag tcgacgctcc gccccacgaa gatggccgcc gcggccagag ccggggccag
    18001 gcccaccacg tcggtcagga agaccgacag gtaggccccc gccatggtgg tggtcaccga
    18061 gaacccgagg tcccccgccc cgtaggcgag cttccgtccc agcccgagct tgccgttcat
    18121 ggcggaaagc atagggcgcc gcctcgggac ggtcaagcga gacgccgcat cctcgatgta
    18181 aggccgcggc gccggttcat tccgagcagt ccgaccggag ggtgatgcgt cgatggtaga
    18241 gatccgtcgg gacctcgaag gagcagcgga atccgtttcc ggccgggaag gtcaccgctc
    18301 ccttgagctg accctccgcc agggccagga tgaaccggaa ccccgaacta tcctgggtac
    18361 gcgggtcgaa gcccgggggg accccggatc cgtcgtccgc gtaccgcacg cagagcctgc
    18421 ccggggccgt ttcgcaggcc tcgatccgga tcgttccctc ccgcccttcc gggattccgt
    18481 gcagcaggga attcgagacg agctcgttca cgatcaggcc gaggggtatc gcgatgtcga
    18541 gcaggatgtc cggggcgtct acctcgattc ggacccggat cgaaccgtcc gaccgtccga
    18601 gctcggagag aatggtttcg gtcaaatttt cgatgtactt tcgcaagggg atgctcgaca
    18661 ggtcgtccga ttccgggagc atccgctggg ccaggatgat ggagtcgagc ttgcctccca
    18721 ggttctgcgc gaaggcttcg accgacttgt ccgggtagtc cgccgccttg agtccgacga
    18781 ggctcttcac gagctgcagg gaattcccga tccggtgggc cagttcccgg aggagggtct
    18841 cctcctggag gagggtcgcc ttgaggcgct cctccgcgcg gatgcgttcc tggatttccg
    18901 ccgtgaggct tttcgtcttg gcggccacct ggcttcgcag ggagaggctc caggccgcga
    18961 agagcatccc cgcaaaggcg atcccggcca gggtgaacca tatccaggtc ggtacggttc
    19021 gctcgggctc cggctgggtc catttccgga acgcccggga atagaaggac tcggggtcat
    19081 tcaccatggc ggccatgagc cgatcgatcc cctcgaggat atccgcgttc gcgtctttcc
    19141 ggaccgcgaa gtgcagggcc acgggggaga agctgatggg ggtgacccgc accggatagt
    19201 ttcgggccag ctcggtgccc agggagtaga tggtgactcc cgcgtccgcg gaaccgtccg
    19261 cgatcgccgc catcacctgg gcgttgtcct cggtgagtac catccggcag gagatcccga
    19321 aggatcccgt atagatgagg aatccctccg tgaacaccga gccccggacg gcggctatct
    19381 tccggccttc caggtcgaag accgtgtgga tcgggaagcg ggggctcgtg aacactacgc
    19441 cgctgtccac gaagaccggg tgcttggaga aatcccgggt cttcgtccgc tctggggtgt
    19501 agcccagggc cggcagaagg tcgatccggc cgtcggtgag ctcggcctgc aggttctccc
    19561 aggtcccccg ctcgaactcc aggttccatc ccagttcccg ggcgaagtgc tccgcgagct
    19621 cgatgaacag acccgcgggc ttcccgtcct tctcgaagac caggggggcg gcggggaata
    19681 atccgatacg gaccgtccgg ggggcgggcg cgagggggcc cgccgacagc gcgaacgcga
    19741 tgaacgccaa aaacgcggcg gcgctccggc gcctcggcgg gcaggtagaa cccatggagt
    19801 ctcctcgaga tccattctaa tcccgcgcca tcgggttctc aagtccggcc gcccccttcc
    19861 cggtcgtacc ggtccccggc ccggggctcc agccgagccc ggtccaggat ccggatccgt
    19921 cctcgggcgc cgtccaggat ccccagatcc cggaacttcc gcacggcccg ggcaagctgg
    19981 cggtagctgg ccccgatgaa gtccgccagc tcccccaggt cgtcggtgcc cacgatccgt
    20041 ccctccggat ccgaggcggc caggagatag ctggccaggc gggcctccac ggggtagcgg
    20101 agattgacgg attccgcctg ggcccgctct cccagcttgc ggcccaggcg ctcgcagagg
    20161 aaggccagga gccggctgta ccgatccacg gatgcccgta ccgtcgcgga gggcagggcc
    20221 aggcacacgg tgtccgtcag ggcctccacg gtcagggcgt acgtgtccga ggtgaacagc
    20281 tcgagctccc ccaggacctc cggggggcgg tagaaggcgg ccaggatgcc ccggccgttc
    20341 tctaggatgc tgtaggcctt ggcccggccc tccacgaaga acaggaggtc ccgggcggga
    20401 tccccggacc gcacgatggg atggccgggg ccgtgccgta cgggccggag ggattcgtac
    20461 aggtcccggg gaaggatgcg gtccagaccc caacgtgctg cgtagccgcc cgtctcctcc
    20521 atgtccgcct cctcggtcgt ttccggtgtc acgtagcttg tgaagagtcc cggattattt
    20581 cagtggatag gacatatgtc ctatccggaa accccattct acccggatac tcgaatccgg
    20641 gaaaggcgga caggatgatg tacacgatac ttgcgtccct tacgggggtc ctgatcaccc
    20701 tcatgaactc ggtgaacagc cggctggccg cgacggccgg ataccccgcg gcggcggtga
    20761 cggcccacgt ggtcggcctg gcggccgtga cgatcctgac cctggcgcgg aaagagcctc
    20821 ccgagcccgg ccgggtgccg ttccgtttct acctgggagg gtttttcggg gtcgggacga
    20881 tcttcgcgtg caacttcgcg ttctcgaccc tgagcgcctc cctggccgtg gccctgtccc
    20941 tcctgggcca ggcgggcttc tccctactgg ccgacgggac cggcctcctg ggccggagga
    21001 agtaccccct gggtccgggc cgcctcccgg gcatagccct ggccgccgcg gggaccgccg
    21061 tcctggggtg gggcggggag ctccgatggg ccgccgtgcc cgcggccctg gccgcgggga
    21121 tcctcccggg gatctccttc atcctcaact ccgaactggg gcggcggcgc ggggtcctgc
    21181 gaagcgcccg ctggaactac ctgaccggcc tggcgggcac ggccgcggtg gccgccctca
    21241 ccgggcttcc ggcggcgccc gcggcccggg cggtcctgga agcgggcccc ctcctggccc
    21301 tgggcggggg gctggcgggt gtggccgtcg tggcgaccat gaacttcgtg ttcccgaggg
    21361 tcccggcctt caccgcgacg atcctcctct tttccggcca ggccctggcg ggcgtggccc
    21421 tggactgggc ggcccgggga gagctcgacc tccacaaact gatcggtacg gggatcgtcc
    21481 tggcgggact ggtgctcgat tcccggatct cccgcaggcc cgcggccgcg tgctaggatg
    21541 gctcccatgc cgcgacgtta cgacacggtc ttcatcgacg ccgacgacac cctcttcgac
    21601 tatccccggg cggaggagcg cgccttctcc cggacctgcg tccgcttcgg cctttcggac
    21661 acgccggaac tccgggccgc ctaccggcgg atcaaccggg agcaatggaa actcctagag
    21721 cggggaggga cggaccaggc caccctgcgg gtcgagcggt tccgcctgct cttcgaggag
    21781 accggggacc ggctcgatcc tgcggccgtg ggcggggcct acgtggagga gctctcgcag
    21841 ggggccgacc tccttccggg ggcccgggag atctgcggat acctggcgga gcgttaccta
    21901 ctggcgatcg tgaccaacgg cctggccgac gtccagcgct cccgactggc cctctcgggg
    21961 ctcggggacc tcgtcgcggc cctggtcatc tccgaggagg ccgggacctc gaagccggat
    22021 ccccggatct tcgagtacgc ctgcggccgg gtaggccgga ccgaccgcgg gcggatgctc
    22081 atggtcgggg attccctgga gtccgacatc cggggaggga ccgccttcgg gatcgacacc
    22141 tgctggtaca atccccgggg gatggagaat cccacggaac tccggccgac ctacgagatc
    22201 cggtacctgg cggagctgcg gagcatcctc tgacccggag tgtccggtca gccgttcacg
    22261 gatttggcac ttcggccgcg gacttgcggg gaccgggggc ccgccgctag aatgggagtc
    22321 tgggtcgagt cggcgcgggg cgcctggtcg gccccggacc cgagatcggt ccggccggtc
    22381 gatccttccg cggaggtggg gcatgaagaa caggggcggt ggttccgcct tcaagatcgc
    22441 ggccttcctg gtgcccgtgg gggtgctctt cgcggctttc ttcctctacc ccttcgtgtt
    22501 caccctcttc gccagcctga cccggtggag gggcatcggg gccatgcgct tcataggcct
    22561 ggggaacttc ggccgactcc tgggggatcc gaccttccgg ctggccatcc gcaacaacgt
    22621 gacctgggcc ctggccgcgg gcttcgtcca ggtgcccctg gccgccctgg tcgcgatact
    22681 gctggcccgg cgtccccggg gctggaagtt cctccggacg gtctacttcc tgccgaacgt
    22741 gatctccacg gtggccctgg ccatggtgtg gtccgccctg tacaaccccc agtacgggtt
    22801 cttcaacggc cttctgaaac tggcgggctt cgaggcccgc aacttcctgg gagatccgtc
    22861 cacggccctc ctgtccgtga tcgtgtccag cgtcctctat atcgggtact tcatgatcat
    22921 cctcctggcc gcggccatga acatacccca ggagctctac gaggccgcca agatcgacgg
    22981 cgccaacgcc ttccagcagg agttcctgat cacggtgccc atgctccggg gaaccctggt
    23041 cacctccatc accctggcca tggcctacgg gatgcggcac ttcgaggcca ccttcctcat
    23101 gaccgggggc ggtcccgcct attccaccac gaccatgggc atagacttgt tcctcaagat
    23161 ggacgccctc cggtacagcg aggccagcgc cgccggggtg atcctgatcc tcctgggaac
    23221 cgtggtgatc gtggcgatcc ggaagatcct ggggaacaag gaccccatgt cggaggcggg
    23281 acaatgagcg ggacccccgg atccggaggc cttcgggccg gtgccgccct cgcggtcctc
    23341 ctcaagtgga tcgtgctcgt cttcttcctg gccgtggccg tcctgcccct ggcctggctg
    23401 gtcctgaact ccttcaagac gaacctggag atccagagct cccccttctc cctgcccgcc
    23461 cggccggact tcgagaacta cgccaacgcc gtggccctgg ccgacctgcc ccggctcttc
    23521 ctcaactcgg tgatcgtggc ggtcgggacg gtcttcctga acctcgtcgc ctcctccatg
    23581 gcgggtttcg tactggcccg ggagtcctgg aagggccggg agacggtgcg cacggtcctg
    23641 gaggcggggg tcctggtccc catcctggcc ttcatggtgc cctacttcac cctggtgacc
    23701 cgcctgggcc tgtacgacaa gctcgtgacc ctggtgctgg tgtacgcggc catcaacgtg
    23761 ccggtgtcca tcttcctggt gacctccttc atgcgggcga tcccgaagga gatggaggag
    23821 gccgccatca tcgacggctg ctccttcgtc cagcgcttca ccaaggtggt cctgcccctg
    23881 tcccggtcgg gcctggtgac cgctggaacc ttctgcttca tctacgcctg gaacgagttc
    23941 ctgatggcca tgctcctgac ctccagcatc tccgcccgga ccatccagct ggggatccgt
    24001 ttcttcacca gccagttcat cacggactac gcggggatgt tcgcggccat cgtcctgtcc
    24061 atcctgccct cggtcctggc ctacgtcttc ctgcacaaca agatcatcgc gggcctcacc
    24121 gcaggcgccg tgaagggcta acggcccgga gtgtccaatt agtcggttac ttgttggaca
    24181 agcccatgaa ggagcggata tggacttcga gcgaaagcgc ttcgggaccc tgtcgaccgg
    24241 cgaggaggtc gacctctaca tcctcaagtc cgccgacctt tccttgggga tcaccccgta
    24301 cggggcgacc ctggtgtcct tggtcgtgcc ggggagatcc ggccgggacg atgtgctcct
    24361 aggcttctcc accctgggac cctacacccg gaagcatccc tttttcggct ccacggtggg
    24421 gcggttcgcc aaccgcatag ccgggggacg attcggcctt ggcgggaagg actacgtcct
    24481 ggcccggaac aacgggccga actccctgca cgggggcctg cggggcttcg acaagcacgt
    24541 ctggaaggcg gaggcctacc gggaggacgg gggggtcttc gtgcgtcttt cccacacgag
    24601 cctcgacggg gacgaggggt acccgggaac cctccgggcg gcggtgacct acgggctttc
    24661 cggggacaac gaggtctccg ccctctacga ggccgaggtg gatgccccct gcccggtcaa
    24721 cctcacgaac cacgcctact tcaacctggc cggggagggc cggggcacga tcctggacca
    24781 cgaggccgcc ctcttcgcgt ccgcctacct ccccgtggac gggaccctca tcccgaccgg
    24841 cgagctcgcc cccgtagcgg gcggtccctt cgacttccgg acccccaagc ccatcggccg
    24901 ggacctcgag gccgcgggag ggggttacga ccactgcttc gtcgtggacg gggtccccgg
    24961 gaacctccgg ccctgcgccc gggtgggcga ccccgggagc ggccggaccc tggaggtctc
    25021 gaccacccag ccgggggtcc agttctacac gggcaacttc ctggacggga tcccgggcaa
    25081 gcggggggcc ctgtacggga agcacgcggg tttctgcctg gaaacccagg gcttcccgga
    25141 cgccccgaac caaactacct tcccggactg cgtcttcggc ccggttcggc cctaccggga
    25201 gcggacggtc ttcaccttcg ggtactgagg agcctcaaag gaacggcccg ccgttctttc
    25261 gaaggcgggc cgtttttgtc tcggcgcaga gagctcccgg gaccaggcta acccttgacg
    25321 cctcccgcgg ccacgccgcg gatcaggaac ttctgcatcc cgagggtgaa cacgaagacc
    25381 gggatgagca tgatcgtgct ggccgcgcac atctggcccc aggtcagggt gtcccctccg
    25441 atcaggctgc tgatggccac cggagccgtc cgggcgttca cccccgtcag gatcagggcg
    25501 tagaggaact cgttccagct gttgatgaac gcgaagaccg cggccaccac gagtcccggg
    25561 gcggccaggg gcaggaagat cagccggaac atcccccagc ggctgcaccc gtccaccatg
    25621 gcggcctcct cgtagctctt gtccaccacc gtgaagaagg ggatgagcat ccagatcacg
    25681 taggggacgt tgaaactcga gtgggccagg gtgatgccga agatggtgtc ccggatgccc
    25741 acggtgcgga acatcaggta cagggacacc accagggaca tctggggtag catgcggaag
    25801 accaggaaac tgtaggccag ggcgttcttg ccccggaact tgaaccgggt gagcccgtag
    25861 gccgccatgg cccccacccc cacggagagg gcggtggaca gaaggcccac gatcacggag
    25921 ttccgcatgg ccctcatcag ctggccgttc tggaagacca gggcgtagct ggctccggtg
    25981 aactgcttga gcgagagctt ttggcccgag aggatcacga tgtcccggcg gaagccgttc
    26041 agcagggtga tcgcgatggg gtagagttcc accgccagga ccgccagggc gaagacggcc
    26101 accgcggcgg tgatgatttt cttgcgcgtc gccagcctca tcggttctcc ccctgctcct
    26161 cgagcttttc taggctcatg ttcttccgga ccagggtgac cgcgaagagg atggagatca
    26221 ccagaaggaa gatggccatg gcgctggaca ttccgacctt catgtacccg aaggcctggc
    26281 ggtgggtgta caggctgaac agggtcgtgg ccatgcccgg tcctccctcg gtcatcacgt
    26341 agacctcgct gaaaattcgg agggcgtcga tgatgcgcag gagcagggcc gtcaggatgg
    26401 tggggtagag caggggcagg atgatcttgg tcagccgttt ggtcgaggtg gctccgtcga
    26461 tctccgcggc ctccaggatg tccgcgggga tgcccaccag gcccgcgtac aggagcatga
    26521 agacgaaggg ccacatgcgc cagacgtcct gcacgatcac cgccgcgtag gcgtaggtcg
    26581 gatggcccag ccaggtgatc tccggggctc ccagggacag gaggaaattg ttcagggcgc
    26641 cgtaccgagg ctggagcatg aagcgccagg tcatgcccga cacgatgggc aggaagaaga
    26701 ccggcaggag gatgagggcg cggaccacgc ggcctccccg gaattcccgg ttcaaaagga
    26761 gggccgcccc caggcccagg gctatctcga tcggaatggc gatgaagatg aagcccagct
    26821 ggagccggag gctgttccag aactccttgt ccctcaccag ttccacgtag tttcccgccc
    26881 cgacgaagcg ccgggttccc ccgcccgcgg acaggggcca gtcgaagagg gagaccacga
    26941 aggagaagac gatgggaaag atgaggaccg cgaacacgat gaggaaggcc ggtatcaaaa
    27001 acgggacggg ggagccgtag ctccccgtcc cgtttccaga agcggcgttc ccggaagggc
    27061 tagtagcccg ccttcttgag aatgtcattg gtttcccgaa cgatggcctg gacggcgtcc
    27121 ttggtgggca tctggccggt gattcccatg atccagtact tcttcaccac gtcctggacc
    27181 tgtccgatct ccttgaacct cggccgggag gcggccacgt tcatggacgc ggcgagggcc
    27241 ggcagccaag gcttgctgga gacgaaggcc gggttggtca gggactcggt gcgggcgggg
    27301 atcgtgttgg cctggaccag cttgtccgcc acggccttgc tggaccacca gtagacgaac
    27361 tccgccgcgg cgtcctggtt cttggaggcc ttggggatgc agatggccca ggcgccggtg
    27421 acggcggcct gtccgcgggg tcccgcgggc ggggcggagt aggcgatctt gccggcgacc
    27481 agggacttgt tcgggtcctc gtaggcggcg atctgggccg gccagatgaa catggagaag
    27541 acgcccttgg ggtccgcggc gaaggcggcc tggcgctcca cgtgggccat ggtctcggca
    27601 cccttggggg agatggccat cagcttcttg tagaactcca tgccctcgat ggccttggcc
    27661 tcgtccagga cgaccttgaa ggtcccgccg gtgtagtcga tgaacttggt gccgtgggat
    27721 tcccacatga acacggcttc cgagaggacg ccggcgggtc cgccgttgaa ggggccgaag
    27781 ccggcggcga tcccgttctc gtggaagaag gtccccattt caaggagttc cttgaaggtc
    27841 ttggggatgc cgaggtcgta cccgtacttg gccttgaact gggtcttgta ggtcgggttc
    27901 tcgatcaggt ccttccgggt catccagaca tagaggttcc cgttgatcgg gatgcccagc
    27961 tggacgctgt tccaggtgcc gatgctcagc atcttggggg tcacggactt cggatccgtc
    28021 ttgggataca ccttgcccgc gggccactgg gcgaagggct ggagcaggga ggcgaagacc
    28081 gggacgttgc cctcgtcgat gccgaccacg tcgtaatcgg tgctggtggc ggtcacggcc
    28141 acggcggtct tggtcaggac ctgggggggc tggagcatct cgatgttgac cttgatgttc
    28201 gtggccttct cgaactccgg cagcatggcc accagggcct tcggataggg atcgtcccag
    28261 agcaggacgt tgatgacctt ctgctgggcg gacgcgaacg cggcggccgc caggagggca
    28321 ccgagcaaga cgcttttctt caggttcatc tcgcacttcc ttttttcgct ggacatgggt
    28381 tccggaagcc taccgcattc cgtctgccgc cgcccgggat cgatcccgat tggggacggc
    28441 atattagtta cttcatgtaa ataatacccg gtattggaat cctatcaaca aaaaacggtg
    28501 aaatccgagg ccgcgacctc cctcggcggg cccggcactc gggcccggcc ggccttgccg
    28561 tgggaccgga aaccccactt tgtccttgac aggtccggat tctcccccta ggatcgattt
    28621 acttacactt gaaaagaaat atgtccgcga ggaaggctat atgtccagac tggagatcat
    28681 caggaccgga aacggcttcg aggttcggct ggggggccgc ctggcgatcg agcacggacc
    28741 cggccggccc ttcctgtccg cgggccgggg ggaaggccgc tacgacatgc accacggcaa
    28801 cttcgaggtg accgagagcc tcgccgagct cgttgccctg gaggacttcg agttggcccc
    28861 ccggccggac gggggggccg aggtcagctt ctcccgccgg gggctctacc ccgtgcgggc
    28921 ggacttctcc gaggtccggg gccgcctggt cgtctcgttc cgggccgaac ccggggaggg
    28981 cggatcggcg ccgcccaacc ggtaccgggt gcggcttccc gccgagccgg cggagcacgt
    29041 ctacggggcg ggggagcaat tctcccattt cgacctccgg gggcgggtct tccccctgtg
    29101 gaccagcgag cagggcgtcg gccgcaacaa gctgaccgag gtgacccgcc tggcggacct
    29161 ccacgacaag gccgggggcg attactggtg gaccttctat ccccagccca ccttctgctc
    29221 cagccggcgg atcttctacc atttcgacgc gaccgcctgg gcgtccttcg atttccgctt
    29281 cccggaccgc cacgagtact acgtctgggg cccgcccgcg agcctgaccg tgggggccgc
    29341 ggattccatg ctcgacctgg tctcggacct gacggacctc ctgggacgcc agccggagct
    29401 cccggactgg gcccacgacg gggtggtcct gggcatccag ggcgggaccg agacctgcct
    29461 ggccaagctg gcccgggccc gggccgcggg ggtccccgtg tcggggatct gggcccagga
    29521 ctgggaaggg atcaacatga ccagcttcgg ccagcggctc cgctggaact gggtctggga
    29581 tcccgagcgg taccccggcc tggacgaggc gatccggagc cttcgggccg aaggcgtccg
    29641 cttcctgggc tacatcaacc cctacgtcgc cgcgggcaag tccctcttcc gggaggccgc
    29701 cgcgaaggac ctcctggccc gcagggcgga cggctcggtc tacctcgtgg acttcgggga
    29761 gttcgacgcg gggatcgtgg acttcaccaa gcccgaggcc ttcgcctggt acaagggggt
    29821 catccggaag aacctggtcg agttcggcct tgccggctgg atggcggact tcggggagta
    29881 cctgcccacg gacgccgtcc tggcggacgg gacccccgcg gagctggccc acaacgagtg
    29941 gcccgccctc tgggcccggg ccaaccggga ggccgtggac gaggcgggcg cgtcgggaga
    30001 gatcgtctac ttcatgcggg cgggctggac ggggagccag aggtggtgcc cgatgatgtg
    30061 ggcgggtgac cagaacgtgg actggtccag ggacgacggg ctcccctcgg tgatccccgc
    30121 ggccctgtcc ctggccatga gcggccacgg cctgcaccat tccgacatcg gcggctacac
    30181 gaccctgttc atcctcaagc ggaccaagga gctgttccag cgatgggcgg agctggccgc
    30241 cttcaccccc ttcatgcgga gccacgaggg caaccggccg ggggacaact ggcagttcga
    30301 ctccgacgag gagaccctcc ggcacctggc ctccatgacc cgcctgcacg tagccctcaa
    30361 gtcctatctc aaggactgcg tccgccagaa cgccctgcgg ggacatcccg tccagcggcc
    30421 gctcttcctc cacttcgagg aggaccccga ggcctggggc atccaggacc agttcctcct
    30481 gggcccggac ctcctggcgg ccccggtcct ggaggaggga gcccgcaagc ggcgcctcca
    30541 cctgccgccc ggggaatggg cggatttctg gtccgatcga gtcgtccgct cgccgtcccc
    30601 ctcgggcacg accctggagg tcgaggcccc gatcgggagg ccccccgcct tcttcaaggc
    30661 ttcctcggcc tggaaggacg tgttccggaa ggccgcggac gccgcgtcgg gggccggccg
    30721 gtgaaccatt tccggcggga cgcaccgggc tccggggacc cgctccgctc ctcggacgtc
    30781 cgcaggcgga acgagaacct ggtgctacgg ctgatccggg cctccgggga ccggggactc
    30841 tcccagtccg aggccgtgac cgccacgggc ctgcgcgccc cgaccatgct gcgtatcttt
    30901 tccagcctgg aggagtcggg ctacatcgag tccttgcccc cggcggcaac ggaggaggag
    30961 gggaaggagc gcaagggccg tccccccgtg tccttcgcgg cccgggcgga cgccctctat
    31021 accctggggg tcgagttctg ggtggaccgc gtctccctgg ggctcttcga cttccgggga
    31081 aaccacctgt cctccagcgt ccttgccctg ggcggcgggg tggacgcgga ttccgtgacc
    31141 gaggtcatag ccgggggcgt ccgagccgcg atgtccgccc ggggcctggg cggcgagcgc
    31201 atcctgggcc tggggctggg ggccccgggg caggtggacg tccgcagccg gcggatcacc
    31261 ttctactcgc ggatacccgg catgcgggac taccccatgg cggagatcct ggaacgcaag
    31321 ctcggcatcc ccgtccacct gcacaacaac tgcgcggtca tcgccctgtc ggagtaccgg
    31381 tacgggaacg tcgggaagac ggactccctg ttcatgttcc tcctgcgctc cggggtgaac
    31441 ggggccttca tcgaccacgg ccgcatccac ctgacctcgg acgggacgac cctggagtcc
    31501 gggcacgtgc ccatcgatta ccggggcaag ccctgcgtct gcggggcccg gggctgtctg
    31561 gaaccctacc tggcggactt ggccaaggag gacctggcgg cggggcgcta cctcttcgag
    31621 ggcctgggtc ccgccctgga ggcgggggat ccgaaggcga ggaaagtact caggcaggcc
    31681 gcggactacc tggcctcggc ggtgcggagc gtacgcaggc tcctcaatcc ccgggccttc
    31741 ctcttcgtga ccgcctccgc ccccgtgtcc gaagccctgg cccggctcgt cccggaaagc
    31801 ctggcggagt ccccctcggg gttcgacgat cccccggcgg ccttcgtcgg gaccgtctac
    31861 gatcccggac tggtccagcg gggcgcggcg gatctggtcc tggacgcctt cctgggctga
    31921 gggccgctac cggccgccgg atgccgcgat cttcgtgacc ccgagctctc cgcccgcctc
    31981 caggaagcgg tccagggcca gggcggcccc ccccagggcc ggcagggcgg ggtccagccc
    32041 ggacctccgg atctcgcagg ggcggccggt ggactccacc cgggaggcga tctgccccaa
    32101 cagggcctcg tcccggaagg gcaggccgta cagggcgacc cgggaaggat ccaacagctc
    32161 gatcgcgttc cccagggccc gggcgaagcg ggcggccagg agggaatagc ccgccggatc
    32221 ctcccggtcc agccgatcga gaagggtctc gatcccgttc cctcgacccc ggtccccgcg
    32281 tccgaaccgg gcgcgcaggg cccccaggga gaccagggac tccagacagc ccctgcggcc
    32341 gcacatgggg cagaccggtc cgtcggcttc caccagggtg tggccgatct ccccggcccg
    32401 gttctgggct cccgcccagg gcggcccacc cgcggtccag gccccgccca ccccggggcc
    32461 gtacttgacg aagaggagcc ccgggggcgg gtcccgccgg gcctccgtca ggaggagctc
    32521 cgccagggcc agggctcgga cgttgttgtc caccgagacg ggcaccccca gggcggccgc
    32581 gaggggagcc gcgagggaga cgggaacgtc ccagatccgg ggttcccgga ggcaggtccc
    32641 ggtcccggga tcgacccgtc ccgtcacccc caggccggct ccgacgactc ccgcggccgc
    32701 gggtccggct tctccgagga tgtccgacgc cgacccgagg acggcctccg ccagccgggc
    32761 tcccggctcg gccttccggc tcccctcccg ggaatccggt cccggatgcc cgccggctcc
    32821 ggggcccgcg aagtcccccg gggggagctc ccggaccccg aggacccggc cggcgaggtc
    32881 ggtgaccagg acctggagcc ggtcgggctc caccccggcg ccgatcaact tgccggcgcc
    32941 gggccgtatc ctaaggaaga ccttgcgacg cccggccttg ccgccggggc ccgaagagcc
    33001 cgcctcctcc aggagcccct cgtccagcag ttccccggag aggatggtca cggaggcccg
    33061 ggtcaggccc agggccccgg cgaggtcgtt gcgggccagg gccccgtggc gatggatcag
    33121 gcggaggatg cccgaccggt tgcgcctgcg gatgtccgtc gcggagagtc cccgaggggt
    33181 catggggcgc gccccgagcg cggcatgtcc ggatttttcc gctcgaatcc accgattttc
    33241 atgggccccc gtacttgcgc gtttccgcgc ggcgtgctag gattggttaa agactttaac
    33301 atataggggc cgatatggca accaaggacg gaaaactttt ccatgcccgg gacagccggg
    33361 cggtgctccg ggagatcttc aaccagcggg actcgaacct cgaattcctc tccctggcgg
    33421 agctggacat cgcggacgcc cgctccacca gcctctgcca caagcgggag gagactctgg
    33481 ccttcaacct ggccgggccc gcctcggtct tcgtggacgg gacggaatac cggatggagc
    33541 actacgacgt gctctacatc ccgaaggggg cggagtactt cgtggaggcc ggggcggaac
    33601 ccgcccacct ctacctctac cgggccctgg cggaaaacgt ccaccccgtc cagctccgac
    33661 gctggaagga tctcaaggac gatccgtccc ggatccgcct cctgacccgg aagaaggtcc
    33721 acctgatgtt cgacgtggcc gagcgggcgg acaagttcat ggcgggctac accttctacg
    33781 aggaccgctc ccgggcctgg ccgccccaca accacacgga ccaggaggag tgctactcct
    33841 tcatcgaggg ctccggggcc atgcaggtct acgaggacga cgagaagctg accttcgtga
    33901 agggggtcga ggtcggcagc cacgtgacca tacccctcct caactaccac cccgtcttca
    33961 gccacgagga gcccctctgc ttcatctggt gcatagccgg cgagcgctac tgggtggggg
    34021 acaagaacaa gtccttcatg gacgggaccg ccaagaaggt gacgacatga tcaacccttc
    34081 ccccgaccga cccgccttgc cggcggagct ggcggacctg gccgtccggg cccggtccat
    34141 ccggagggcc tgcctcgagt ccatcgccgc cctgggggtg ggacacgtgg gcgggtccat
    34201 gtccgtggtg gaggccctgg ccgtcctgta cttccggcat atgcgactgg atccgggaga
    34261 tgccaaatcc ccggaccggg accgtttcgt cctgtccaag ggccacgcgg ggccggcgct
    34321 ctacgcggcc ctggccgaga agggctatct ggaccggcct ctccttctca ccctgaacaa
    34381 gggaggcacc tccctgccca gccacgcgga ccgccagcgg actcccgggg tggacatgag
    34441 caccgggtcc ctgggccagg gcttctccgc ggcctgcggc atcgccctgg gcctccggat
    34501 ggacggcccc cgggcggacg ggacggtccg ccgctgctgg accgtcatcg gcgacggaga
    34561 gagcgacgag ggccaggtct gggaggccgc ggccttcgcg gcccactacc gcctgtccaa
    34621 cctggtcgcc ttcacggacg ccaaccgctt ccagatcgac ggggccacgg aggctgtgat
    34681 gcacctccgg gacctttcgg ccaaatggtc cgccttcggc tggaggacct tccgggtcga
    34741 cggccacgac ctgggggccc ttgaccggac gatcctttcc gcctgctccc cggatcccga
    34801 ggcggacggg gagggccggg gccggccggc catgatcgtc ctagacacgg tcaaggccaa
    34861 gggagtcccc gccctggagg ggaaggccga gagccacaac gcccccttcg ggcccgcgga
    34921 cctcgaggcc gccctggacg cgatggacaa ggaggccccc cgtggctgat cgagagatgc
    34981 gggcggtcta cgcccagacc ctgacggccc tggcggcctc ggaccggcgg atctgcgtgg
    35041 tggaggccga cctggcccgg gccaccggca cgggtccctt cgccaaggcc tacccggacc
    35101 gcttcttcaa cgtgggggtg gcggaggcca acctggtggg cgtggcctcg ggcctgtccg
    35161 ccgcgggcaa gcgccccttc gcggccacct tcgcctgctt cgccagccgc cgggcctacg
    35221 accagttctt cctttccgcc aactacgcgg gcctggaggt ggtcctggtg ggcacggacc
    35281 ccggggtgac cgcggccttc aacggcggga cccacatgcc cctggaagac ctggccctga
    35341 tgcgggccgt gcccggcctg gccatcgtgg agcctgccga cccggtgtcc ctggaagccg
    35401 cggtgaagct ggccctggag cgcaagggct gcacctacct ccggctccag cggaagcccg
    35461 ccccggagat ctacggcgcc ggggaggcct tcgagttcgg aaaggccaag ctattgcgtc
    35521 ccgggaagga cgtggtcctg gccgccctgg gggccctgat ggtcggggag gcccggaagg
    35581 ccgccgacag cctggccgcg gaagggatcg acgccgcggt cgtggacgtc ttcaccttgg
    35641 cccccctgga ccgggcggtc ctcttggact tggcccggaa gaccggccgg atcgtgacct
    35701 gcgagaacca ccgggtgacc gggggcctgg ggtccgcggt ggcggagctc ctggccgagg
    35761 agggcgtccc ggcccggttg gcccggatcg gggccggcgc cgacgagttc ggcgaggtcg
    35821 ggacccagga ctggctggcg gagcgattcc gcctgaccgc gccgcacatc gcggccgcgg
    35881 cgcgcagact cgtgaaggga gtgtaattat ggagaagaag atacggctcg ccctggcctc
    35941 ggacctctcg ggctttcccc tggcccggga gatcgtgaag cacctgaatg aaaaccaccc
    36001 cgagatcgag gtgatcgact tcggcatccc ctccgccgac cagccccagc cctacttcat
    36061 ccaggccccc aaggtggcca aggccgtgca gaagggggag gcggacaagg gcgtcctgat
    36121 ctgcgggacc ggccagggca tggccatcgt ggccaacaag cacaagggcg tgtacgcctg
    36181 cgtggtggac gacatcttct ccgccgagag atccaagatc gtgaacaacg ccaacgtgat
    36241 caccctgggc gggtggatca cggccccctt cctgggctgc cagatcgtgg acgcctggct
    36301 ggccatggca ttcacccaga agatggaatt taagaaggac ttcctgacca acgccttcaa
    36361 ccaggtggtg gccctggaag cggagaactt caagtgagcg cccggtacga ggttccggac
    36421 accgaggacg gcagggccgt cgcggccctg gtggcccggg cccgggcggc ccaggccgtg
    36481 gccgcgacct gggaccaggc ccggaccgac gaggtctgtc tg
//
LOCUS       DAOTEI010000014        33532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_865_length_33532_cov_5.612092, whole genome shotgun sequence.
ACCESSION   DAOTEI010000014 DAOTEI010000000
VERSION     DAOTEI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 33532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33532
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_865_length_33532_cov_5.612092"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="P5313_02395"
     CDS             <1..602
                     /locus_tag="P5313_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRY79245.1"
                     /translation="ETTSDYDREKLQERLAKLAGGVAVINVGAATEVEMKEKKHRVED
                     ALSATRAAIEEGIVPGGGIALIQAALVLEKSDVAKLTDDEKVGWKIVKRALEEPIRQI
                     AENAGVDGAIIADQAKKEKKGVGFDAAKMEWVDMTKVGIIDPAKVTRSALQNAASVAG
                     LLLTTECAITDLPEPKAAPGGGGMGGGMGGMGGMGGMDY"
     gene            complement(1527..1853)
                     /locus_tag="P5313_02400"
     CDS             complement(1527..1853)
                     /locus_tag="P5313_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79246.1"
                     /translation="MASRLSYVLVVVLLSTLVCAGCAPGSDLPSDVVWERDFMTDGEA
                     VLDSDWYFDGGNTGEYSQGFYMNQTDIAAPYLFTGDFEAEFEFYLKVLSGEDIYRYAI
                     RLVDPN"
     gene            2336..3349
                     /locus_tag="P5313_02405"
     CDS             2336..3349
                     /locus_tag="P5313_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY79247.1"
                     /translation="MNDHGAYNRHSLVREMLQTPDLIRSFRLKDAEPVIAAVRETGKV
                     ILTGEGSSRIFPAKNAVAWARRKGLRLDLVTGGSRQASEYDLGDRVVMGASNSGRTRE
                     LIALFEALRKRGHRRLYSITANPGTPLESLSDRTFLLTCGREDAVAATKSVQEMALFY
                     FALLNAAEGLGIDDQLAEAAEAVERALRMPIDGEITEAVAGASTLYFAGRDDGVAEEL
                     ALKTNEIIRRKSGYFEGTYALHGVEEVLNPDDVVLWIEPFEEEYEKIRSALIDGVGMK
                     VLAISEKDTPFPTIRIRGARELTNLVRLAAGWNLLVEAGMRLGIDLDRPARARKVGNE
                     FTG"
     gene            complement(3339..3881)
                     /locus_tag="P5313_02410"
     CDS             complement(3339..3881)
                     /locus_tag="P5313_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRY79248.1"
                     /translation="MRGQAGSEALVIVHSWHHGNTRKVAEALARILGAEVRTPDRVDP
                     ATLGEYDLVGFGAGIDSGRHYRPILDLADRLPPGGGRRVFLFSTCGVPAAFARGEAFD
                     RLVEANHAELRARLADRGYAIAGEFSCVGFNTNSFLKHFGGLNKGRPDAADLARAEDF
                     ARGLLAAPRGERLEPSRTTR"
     gene            complement(3878..4591)
                     /locus_tag="P5313_02415"
     CDS             complement(3878..4591)
                     /locus_tag="P5313_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HRY79249.1"
                     /translation="MNERRTAVAVGGLYILGTVSGVASVILAGFGFGAPDPFTGIAAL
                     GPRMQAGAQAILTMGLALAFVPILMYPILKRESEVSAVGYVVFRGALETVTYILITAC
                     WIVLAGLAPQIVRAGEADGAGLRAAGEAVLRISEAVRMMTVFTFSLGALILYGSFYRS
                     RLVPRWISVWGLAAILVDLGAGFFDLFRGNGRATAVPAWIHFPIFLQEMVMAVWMIVK
                     GFRASAGIPGVVPGKGGAA"
     gene            complement(4584..4814)
                     /locus_tag="P5313_02420"
     CDS             complement(4584..4814)
                     /locus_tag="P5313_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY79250.1"
                     /translation="MSIIFRLDRMMADRKMPLNELARRIGLTDSNLSILKTGKARAVR
                     VSTLNALCRELSCQPGDLLEYVPGGPEEDNDE"
     gene            complement(4826..5317)
                     /locus_tag="P5313_02425"
     CDS             complement(4826..5317)
                     /locus_tag="P5313_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRY79251.1"
                     /translation="MKYFGTRSLSSFMHGLLTVAWYLAWLCAALAIVLGTVIVFHEQL
                     GSPFSRGMAESSRSDIRDWERFQSLPLGVRVLILPYFGAVMTLVLMVLRKTRSLFSNF
                     KDEVVFDAGNVRLIRRTSKLVIALAVLTFSAGSFLTAVILLLICEILAAGTVLKEEHD
                     LTV"
     gene            complement(5482..6588)
                     /locus_tag="P5313_02430"
     CDS             complement(5482..6588)
                     /locus_tag="P5313_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRY79252.1"
                     /translation="MTRPVDSRVLSDLRSFAHRLVDPAAARVAIPPERPERGYWFGGG
                     NMTVGPDGRLFLTGRYRSAGDSRTGLDLGDRGRELAVFASRDGGRSWEKVLSFDKAAV
                     CPDGREALSIEGSALRFDPGRVDLYVSSEKRVPYPAPVEAFQKPGTGIWTIERLSAPT
                     LEGLAGARPRTVAAGADPATLQVKDPFLAERPGNGQYLFFCHHSFNWSSSGTGYLPLG
                     PDGDAEGPPVFDCWPRGPAWDVAITRGTCVLPLPGPPPGARTAPSDWPAGLGLAFYDG
                     GECLRNLDEHASAVRRPRGYSCEELGGLGYFVDGDPTAFLRLSEIFPEFASPDGTGCL
                     RYVDVLAAPDSYIATWQQSRPDGSQALMINRVDR"
     gene            6792..7448
                     /locus_tag="P5313_02435"
     CDS             6792..7448
                     /locus_tag="P5313_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRY79253.1"
                     /translation="MTTIERLPPVRLWIRESGGAWYGVAECDGRLAASSSGRTREEAE
                     RVLTSCIPPGAPRTAGIGPSDFADDAARRLAALEAGGEPDRGFELAPEYYPEPAASVY
                     RLAAAVPPGYAASYGGIARAAGTIAREVGRLMAGNPLYPLVPCHRVVGSDYALVGYRG
                     ATSGPDLRDKLERLRSEARGFREEVELPGAGGLRVFPVEWVLAKADLEGDGGGQPELW
                     "
     gene            complement(7467..8405)
                     /locus_tag="P5313_02440"
     CDS             complement(7467..8405)
                     /locus_tag="P5313_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HRY79254.1"
                     /translation="MDEIRYALVAGAGAIGSAVAAAIHKARPGAVRLLADDRRAEVLA
                     RDGLSVNGERLDLKVLPAGRAPAPGDEPGLVLVCVKHHQLAAAIRDLAPCVGPGTLIL
                     SLMNGIDSEKDLRAAFGEDRVLDAMILAIDAVREGNATNFTVGGTIHFGEDRNPPRAW
                     SPRVARIAEFFDRCGVRYKVPEDMVHAMWFKLMVNVGMNPASAILRAPYSSFQQYPEA
                     RNVMLAAMGELIEISKVLGTGLTEEDIGVWDRTLAGLSPSGKSSMLQDVEARRKTEVE
                     LFAGTVVRLGREAGVATPVNALLFDMIRTIEKGYGA"
     gene            complement(8451..9119)
                     /locus_tag="P5313_02445"
     CDS             complement(8451..9119)
                     /locus_tag="P5313_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79255.1"
                     /translation="MKKVDVPGKAFLTRERVLRAALDMADELGVESLSMRRLGSRLGV
                     EAMSLYNHVEGKEDVLDGILELVIGEIAVPAAGVDWRTAMRERAVSARKAFSLHPWAA
                     ALMDSRTSSGPGRLGYFDAVVGALTGAGFPLERAGRALSVLDCYIYGFALQRRNMASG
                     GRERAEDRAEALRDSAPTEAYPHLARMIEWTVAHGYDEDADFEFGLTLILDGLEGMLN
                     PGRG"
     gene            9313..9966
                     /locus_tag="P5313_02450"
     CDS             9313..9966
                     /locus_tag="P5313_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HRY79256.1"
                     /translation="MQDLQKAGGIAALVAAGTYLFAIGLAATVLGPMADPDLGFREYM
                     TFLEAHKTLVFLWHSVMYFVNGICLAVLVPALYERLKPSSPRLALVASIFGLLWTAFV
                     FLSGVITNHGTEALLALHGKNPAGAEALRTALDAVTLGIDSSDRLLGSLWVGLASLAA
                     FKGKTFPRFLCVFGMLIGAAGLVGTAIPAAVAVAYLFGVGAILWWAGAGVVLIRKRT"
     gene            complement(10113..10928)
                     /locus_tag="P5313_02455"
     CDS             complement(10113..10928)
                     /locus_tag="P5313_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY79257.1"
                     /translation="MKLHPLRKLGFVLVLVLIAGPVLFPFVWMLASSFKTQVDIIAWP
                     PRFLFSPILENYRKVFQEQNFLKYFINSSIVGVTAVAFSLLLGLPAAYSIARFKQKKL
                     SVLILVARLMPGISFLMPWYIIFSRLKLMDSYAALILSHMLIALPIVVWIMSSYFETI
                     PVEMEESAMVDGATRQRAFLSIILPVSSPGIVTAVTLSFIFSWNNFMFSQVLSMERTK
                     TLPIAVYNFVSYAEVDWGAVMAAAVAIMAPAIILTMIFQKYVVKGLTMGAVKG"
     gene            complement(10938..11816)
                     /locus_tag="P5313_02460"
     CDS             complement(10938..11816)
                     /locus_tag="P5313_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY79258.1"
                     /translation="MRKSGILERHLNVLFPLPAVAFVVVMMVFPVLYTLFLSFSNWNL
                     TSGMPFALVGFRSYLRVLREPRFLDALGRTFSFTALALTAETLLGLGLALLLNRKFRG
                     KGFMKLILLLPLVATPVAIGIVWNLFYDPTIGLANYVLSLFGLPRQAWVSGAATVIPS
                     LALVDIWQWTPMITLIVLAGLAGLSPEPYESARVDGATGPQIFFRITLPMIAPTLLTA
                     MILRAIDVLKTFDIIYAMTGGGPGYASETLNVMAFKYSFEYFRMGQSSVVLVFLFLLV
                     LTLSALIMRLRRRFEL"
     gene            complement(12007..13254)
                     /locus_tag="P5313_02465"
     CDS             complement(12007..13254)
                     /locus_tag="P5313_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY79259.1"
                     /translation="MRKLIAILLAVVLVMAVSAQAKEIRVLLANHPYGDLLKTVIAEY
                     EAASGVKVNVESLQESQLTTKLTTEFATNSSTVDVFMTRPLQEGKMFYKNGWYEPLAG
                     YDFSDYPESAMGIARFGAKPYLVPLVTEWQVLYYRKDLLAKAKLKVPTTFAELEAAAK
                     ALKTADIAGFASRGRGAAAVTQLSSYIYNYGGRYLDKGKAVFDSKEALDATRFYGRML
                     ANYGPTGVTSMSWENIMPLFQAGKVAMWTDASVFYGQIVDPAKSQVKAADVGIANLPA
                     GPKASSPFIVVSWGMAVARQSKNKALAMDFIKWATSKELAKKGLIANITMARNSAWAD
                     PEVRAKANAELVASQAFAAKNGYPYDRPYMSAVGNARDLIGEVIIESINTAGTSTKLE
                     QLAKDKVRAVNELLEDTGEFGVY"
     gene            13397..14245
                     /locus_tag="P5313_02470"
     CDS             13397..14245
                     /locus_tag="P5313_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRY79260.1"
                     /translation="MRHTSCLYTIHERLASMSEKERRVADFILDSPQEAVHPSIEALA
                     ERIGVSESTLFRFVRKLGYEGYQQFRIALATETVGPKSGYYDAPVDSESGESVVAVVF
                     KTAIGALERTMGGLDPKAVAKAADLLTRTRFVLFLGLGGSAIVAKDAYHKLMRTGIRC
                     AAPEDFHMQLMAASQTEPGDVALLVSHTGANKDALAIADVLRSRGAVIVVVSSYPRTP
                     LSKMADVLLLSAAPPSPYSIEAFSARLAQLALVDALYVEMMQRRGEEGSQALGEMRSV
                     IAKRRT"
     gene            14357..15397
                     /locus_tag="P5313_02475"
     CDS             14357..15397
                     /locus_tag="P5313_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HRY79261.1"
                     /translation="MKALVLEEYKRFAYVDVPDPDLRGPKDVIVRVRAAAICGSDVHG
                     MDGSTGRRRPPVIMGHEAAGEIVRTGTEVRRFKPGDRVTFDSTEYCGECWHCRRGEVN
                     LCDDRRVLGVSCEEYRRDGAFAEYVAVPERILYPLPEGLSFVRASLTEPLAVAAHAAM
                     LSPPLPGESAAVVGAGLIGLLLIQVLRTSTSGPLFALDTDPDRRAQALRLGADYALDP
                     ADPGALEELRSRCGGRGADKAWEAVGITSAVATAVDCLRKGGALTLIGNASPRIDLAL
                     QKVVTRQLTLRGSCAIAGEYPLALDLMARGQVDVDALISATAPLSEGAAWFNRLYARE
                     PGLLKVVLEPGR"
     gene            15407..16489
                     /locus_tag="P5313_02480"
     CDS             15407..16489
                     /locus_tag="P5313_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY79262.1"
                     /translation="MEELRFALYGYGKVAELHAKALASLAGTARLVSVIGRDKGKREA
                     FAARWGVSARATAAEAADLDHARAVLVTTPHPLHAAHSRESSAAGLHVLVEKPMALSV
                     ADCDAMIAAAAAAGRALGVVSQRRWYPAVRRIREALDSGRLGTPALGQVVMLGWRDEA
                     YYRSDPWRGSWAGEGGGVLVNQAPHQLDLLSWFLGPVVEVSAYWANLNHPYIEVEDTA
                     VAALRFTGGALGSILVSNSQKPGIYAKVHVHGSNGASAGVQTDGGAMFIAGMSGVLEP
                     PVNDLWTVPGEEGKLAFWKAEDEAFFKTVDATWWFFARQVEDFAAAVREGRPPAVSGR
                     DGRETVRLMEGIYGSGRSGGPVRPAP"
     gene            complement(16517..16978)
                     /locus_tag="P5313_02485"
     CDS             complement(16517..16978)
                     /locus_tag="P5313_02485"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRY79263.1"
                     /translation="MTDEDLRISVRRLLARQLFGVLATRGEDGRPHLSIVSFVSVDDL
                     GGIIFATSRSTRKYRYLRDRPEVSLFTDDRRDRMDDLMLVTGVEASGSARELAEPDRG
                     AYKELYLAKYPELAEFADAPDTALVRIEVDRYDVVDHFQHLVVLETGRPAG"
     gene            complement(17105..18568)
                     /gene="gnd"
                     /locus_tag="P5313_02490"
     CDS             complement(17105..18568)
                     /gene="gnd"
                     /locus_tag="P5313_02490"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HRY79264.1"
                     /translation="MEAKADIGLIGLAVMGQNLVLNMNDHGYSAAVYNRTVSKVDEFL
                     ADAAKGRSTIYGARSVEEFCALLKRPRKIMLMVKAGAAVDEFIETLLPHLAAGDVIID
                     GGNSHFPDSTRRTRYLAQKGIRFIGTGVSGGEEGARHGPSIMPGGNPEAWPLVKDILQ
                     AIAAKTADGQPCCDWVGQEGAGHFVKMVHNGIEYGDMQLICEAYHVMKQGLGLDAARM
                     HEVFAEWNKGELDSYLIEITRDILAYKDADGSPLVERILDTAGQKGTGKWTGISSLEL
                     GVPVTLIGEAVYARCLSAMKEERVEAAKVLAGPGSGKISVPDAKAFLEDLRQALLASK
                     IVSYAQGYMLMREAAKEEGWNLNYGGIALMWRGGCIIRSVFLGRIKEAFDRDPNLRNL
                     LLDPYFWSVIERCQGSWRRVVAKAVEAGIPVPALSTALGFYDGYRSERLPANLLQAQR
                     DYFGAHSYERTDAPRGKFFHTDWTGTGGKVAAGTYEV"
     gene            complement(18604..19890)
                     /locus_tag="P5313_02495"
     CDS             complement(18604..19890)
                     /locus_tag="P5313_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRY79265.1"
                     /translation="MLYYAKGSPDTAFTDRELEAAFQEGLDKVEAARGRPKRVLVLPP
                     DITRVHSRAGILTRFAYDRYGSALTDILPALGTHKPMTSEEIREMFPGVPENLFRVHR
                     WRTDVATLGEVPASFIEEVSEGKLSFPWPAQANRMVVEGGHDLILSPGQVVPHEVIGM
                     ANYNKNVFVGTGGAEGINKSHYLGAVYGMERIMGRAINPVRKVLNYAHDRFARHLPLV
                     FAHTVVGRRADGSLAVQGLYIGDDLECFLKAAELSLKVNFTMLDKPLAKAVVYLDPKE
                     FRSTWLGNKSVYRTRMALADGGELVVLAPGVKEFGEDPEIDRLIRKYGYSGTDRVVGL
                     VEKEADLKASLSAAAHLIHGSSEGRFSITYAPGHLSREEVEGVGFRFADLGETLKRYD
                     PARLMEGFNTLPDGEEIFYISNPAVGLWAYRGRFAS"
     gene            complement(19891..20793)
                     /locus_tag="P5313_02500"
     CDS             complement(19891..20793)
                     /locus_tag="P5313_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRY79266.1"
                     /translation="MSATTNDPSQVLRDLKPEHEFFIGIDSDGCVFDSMEIKHKECFC
                     PAFVNHYGLQAVSKYAREAWDFVNLYSKTRGCNRFPAVLRALDLLRERDETKARGVRV
                     PELAGLRDWVRRETKLGNPTLKAELDRTGDPDLRRVYAWSVDVNETVARIVRDVPPFP
                     LVRESLAAMEERADVIVVSQTPQEALQREWEEHGIAGHLRVIAGQEMGTKTEHIAFAS
                     KGKYAPGHVLMIGDAPGDLEAAKKNGALFFPVNPGHEEASWKLFHEEALGRFFAGTYG
                     GEYESALIAEFDRYLPELPPWKGR"
     gene            20930..21781
                     /locus_tag="P5313_02505"
     CDS             20930..21781
                     /locus_tag="P5313_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRY79267.1"
                     /translation="MTHLPRHAEILKILSLLRNVSVGDFAQRLGVSEVTIRKDLAQLE
                     EMGCLIRTRGGAQIAEDTRVLKTMDVRLKERMDVKEAIARKARELVRDGETVYLDAGS
                     TCLLLAKELTEMNLRVVTNSIDVMAALGACPGISLISLGGTYRREAGSFVGPMAIEAL
                     RTLQIETCFLGATGFSNRGAFSSQNLIESQLKQKVLEASGRRIILADSGKYGRQAFSV
                     FARAGDVDILVTDPGFRNIKEFNVFGMEIVLATGIAASDPSRGPESGSSAGFGEFQAR
                     KTAGIGE"
     gene            21852..23081
                     /locus_tag="P5313_02510"
     CDS             21852..23081
                     /locus_tag="P5313_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRY79268.1"
                     /translation="MKGNCVVAQSGGPTAVINSSACGVIQEALKSPDITGVYAAHNGI
                     LGILNETLFDLGKEDPADIEGLKTTPSAALGSCRYKVKTEEDYGKILAVFKKFGVRYF
                     FYIGGNDSMDTADKVNRLASREGYEFRAMGVPKTIDNDLAFTDHCPGYGSVIKYLATM
                     VMEAGRDTEALYTADTVNVIEAMGRNAGWIAAGTALAKRSEEDAPHIILLPEVPFDPE
                     AFKDRVNHYLKTIKRCVIVVSEGTKNPDGTYISEQKGQFAKDSFGHVQLGGAANYIKA
                     LVEAEVKVKARFAIPSTIQRAGAHFASLTDSQEAYETGRQAVRLAVAGESGKMVTLVR
                     DSDDPYRWSTGLADLSQVANGEKYFPKEWIGADGFSVTDAFLRYARPLIQGEVPVPIE
                     GGLPRYVRFNKHFVKSV"
     gene            23205..24029
                     /locus_tag="P5313_02515"
     CDS             23205..24029
                     /locus_tag="P5313_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY79269.1"
                     /translation="MTYLEEMFSLSGKTVAITGGGGVIAGAMSEAFLRAGATVALWDI
                     KLEFAEAARQRLAALPGLSDAADRILPVEVDALSEPSVRKALEATADKLGGPHVLVNA
                     AGGNKGKSAFLEIDVPLFEDILRLNLVAGLVVPTKVTAAFWIEKGIKGNILNMASMGS
                     YIPLSGVWAYDAAKAGVMNLTMACAKEFAPHGIRVNAIAPGFFLGNQNRALLVDEKTG
                     DLTPRGKDVIAHTPFGRFGDVSEIAGISLYLASDKAAGFVTGITVPVDGGYLVQNI"
     gene            24031..25452
                     /gene="uxaC"
                     /locus_tag="P5313_02520"
     CDS             24031..25452
                     /gene="uxaC"
                     /locus_tag="P5313_02520"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006118302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRY79270.1"
                     /translation="MASFIHEDFLLSGENARALFHDHAEGMPIFDYHCHVPPAQISEN
                     TRFENLTRVWLGGDHYKWRAMRTAGVPENLITGDAPDREKFQAWARTVPRTLGNPLYH
                     WTHLELKRVFGIDELLDEQSADRIYDRCTALLAREDYRVRGLVRRFGVAGICTTDDPT
                     DTLEHHAAIAADESFPVKVLPAFRPDKAHALESPERWKAWIEALEAASGVPVTDYPSL
                     VAALKNRHDFFHARGCRISDHGLQFVPAPSASPADRDRILKEAKAGRRPSDADILAFR
                     GALLTDLGRMDAEAGWTMQLHVGALRNVNPRMFASLGPDTGYDVIGDWPQAEGLARLL
                     ADLDQDDRLPKTILYVLNPRDNEVLATMAGAFQDGRTAGKIQFGSGWWFNDQKDGMER
                     QMTALSSMGLLSAFVGMLTDSRSFLSYPRHEYFRRILCNLVGGWMDRGEIPPDLEAAG
                     NLVKDVCFRNAASYFGIDLGVKG"
     gene            25510..27126
                     /locus_tag="P5313_02525"
     CDS             25510..27126
                     /locus_tag="P5313_02525"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HRY79271.1"
                     /translation="MERNDLRIQRNRELLLAALDRQGLPDAKPEALAAHRERSFGRTD
                     LGGGVFYYPRSLFAAGSLRFLLAGTGIPAAPDAPMNERDGKRLLVLSKDAIPPAFEGE
                     RFSAAGWKVLDGPRSWGNYQAIKEHLPFLAPTSLRGERTTIGCGDRLGMAGPGHIRAA
                     RGFRVSPVLAQLSMRELKLTGRTYPRVVKDAAFAALQEGFDRGFGADGDHLKNIPDID
                     TALDAGMPMITLDLTEVMDPAPASWTEAAVEEAFGRLAEPVRRRVLDGYAGKTFALAG
                     ASIVVSDLEAKRCALMYWKALEFTGEVDRHLRSRRGDAYDLEVSIDETTAPTVPAHHL
                     FIASELARREVTVNSLAPRFIGEFQKGIDYIGNPGDFERQFAVHCAIAKAYGGYKISI
                     HSGSDKFSVYPAIGRHTGLRVHVKTAGTSWLEAVHAASLQDPGLFRDLLSKAYEYFPD
                     ALKLYHITPDLSRIPDPKGVADEDLPGYLERPESRQLLHVSYGGLLNDPDTGPRLFRF
                     LIDREELHYGCVGDHIHAHIERLGAPPHGR"
     gene            27116..27847
                     /locus_tag="P5313_02530"
     CDS             27116..27847
                     /locus_tag="P5313_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY79272.1"
                     /translation="MGDREDTLRFLDFDGVLCDSLPECYAVSRTAYWTGFLGLPEPPA
                     SVPEEAEFARMRPLIRRGADYLLLHLAMREGLRLVSQGDFDALAAREAERDGLFHDLF
                     YQARSDLLRRDPERWFALNPLYPGIREILLRNRANRAVLILSTKEAPFILAILRHHGV
                     EWEADRVLCSGKESKLRFIDRILSERGARGAVFVDDQVDHFQGPSEHPVRRVLADWGY
                     VRPEWIESGTFETVSLSRLPELLPA"
     gene            27781..29361
                     /locus_tag="P5313_02535"
     CDS             27781..29361
                     /locus_tag="P5313_02535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY79273.1"
                     /translation="MDRVGDIRNGLAEPAAGTSAGLGAAAGPAPGIPGKPGIRIGIRA
                     KLLAFYGAAILAVVLLDLSVQVVSFGAIRQFEGNLARYHAIHRLRAGLSAQYARVERQ
                     MRESPDAGSLSLEQDLQDFLMVIQHLEETEPESLEAFFALQATHRGLPAYFEKLEAAI
                     RRRLANDRDWYQDMAWAGRIAGYMDGYLSTFLSEAMQYGDARYQALTRRLNSLRRLTL
                     GGLVVFVVLFGMAAVAISSSVSAPIRRLARASERIAAGELDVPEVRADTGDEVEILAR
                     SFNTMSKSIASMVVDLRGKAELERSLREEERQLMDKERALREAQFISLQDQIQPHFLF
                     NALNTIARTALLEGAKRTEALSLALGRLFRYALGAPESMVTLREELAIVEEYLAFQRL
                     RFGERLRWTVEAAPELQDVRIPRFTIQPFVENAVRHGIEPREEGGSVEISARIRKGRL
                     LLDIRDTGVGMAAAGRGSSGGRSPGRAGEGIGISNVRKRLELRYGPAARLTVRSERGQ
                     GTAVRIGIPAERDGGAPS"
     gene            29358..30665
                     /locus_tag="P5313_02540"
     CDS             29358..30665
                     /locus_tag="P5313_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79274.1"
                     /translation="MSFRILIADDEQLERQALAHILGGLSDRGVELVEAANGRQAVAA
                     ASGGGIDLAFLDIRMPGMDGLEAARILRDADPGIHIVFVTAFDKFDYAREALRLGVDE
                     YLVKPAAADEVLAMARRILDRLERERLGPGASSDGALSLLEDELRADLGRDLGRDLGR
                     DLGRDADRDAEIRERLAAFLRLRGCQGRIAAALVLRPAGPAAPEPGPFPRRPIGRGTR
                     ELLERRLREAGWYALAGEGEPELRCLAVAPSSEGAEAEAEEALWAVLAALTEELRRSL
                     GIRVLTGACLSPDGSPRFREARQAAALARIDRPVVILTDDPGPHAASEDADGPGAGAV
                     ERAVDYLREHLAENLSLVDVARAVGTAPSHLSRLFRRHRGETFVQVFCRLRVDAAKRL
                     LRTGAYRVKETASLVGFKDTAYFCRVFRKYEGASPSEYQAGSG"
     gene            30797..31774
                     /locus_tag="P5313_02545"
     CDS             30797..31774
                     /locus_tag="P5313_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY79275.1"
                     /translation="MSLKKALLLALIVVLAVTSVAAQKKPIVLRLAETHPADYPTTLG
                     DQKFAELVKERSKGRIEIQVYPGSQLGQERAVIEQVQFGAIDFTRVSISPLASFVPVF
                     NALQLPYLYRSEDHMWKVLLGPIGKELFASLEPSGFIGIGWFEPGARNFYNSKRPVSK
                     PEDLKGLKIRVQESELMVGMVQGLGATATPMPYGEVYSGLQTGVIDGAENNWPSYEST
                     SHYEVAKFYTLDEHTRVPEIIIGSKISLSKLSPKDLELIKQAALDAIPYQRKLWAERE
                     KASEAKVRAAGNTITVITDKAPWQAAMKPLYDKQPAAIRYLVQRISEVK"
     gene            31854..32399
                     /locus_tag="P5313_02550"
     CDS             31854..32399
                     /locus_tag="P5313_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY79276.1"
                     /translation="MARRIVAFFNLLHIALLTLAKFMMVAMVLVVFVNVVLRYGFNSG
                     LMWSEEVALLLAVWFGFISLALGVKQNLHINLTLISEHRMSDRANRVLDILRGLVYLA
                     IGLAMLVFGWKLSKMTMGSIMPATLWPAGILYAVLPLSGFVVIYEAAADLAGIDTRDA
                     AVDKYLSGEGTLRDATGGPDA"
     gene            32392..>33532
                     /locus_tag="P5313_02555"
     CDS             32392..>33532
                     /locus_tag="P5313_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY79277.1"
                     /translation="MPDFTFAVIYLIGSFLVLLLLRVPISFALAFSSLSTAALLGIPL
                     MAVVQQMVQGIRSFSLLAIPFFILAGEIMSQGGISRRIVLFANVLVGRVRGGLAQVNC
                     LDSMFFGGISGSAVADVSSIGALIIPMMKEKGYDDDFSVALTVTSACQGVIIPPSHNM
                     IIYSLAAGGVSVGRLFLGGFLPGMLLGFSLMILSYIYALKRNYPKEKKYSLKEALLIT
                     KDAFLGLFTAVLIMGGVISGVFTATESAAIAVVYAFFITFFVYREIPLRAMKGILYNS
                     LKTLAMVMALIASASAFGWMMAYLRIPAMATELLLSITHNKTVLLILVNILLLLLGMI
                     MDMAPLILITTPILYPVFVGALGMSPVHFGIMLILNLAIGLTTPPV"
BASE COUNT         5689 a        11350 c        11091 g         5402 t
ORIGIN      
        1 aggagaccac cagcgactac gaccgcgaga agctccagga gcgcctggcc aagctggccg
       61 gcggcgtggc cgtcatcaac gtcggcgccg cgaccgaggt cgagatgaag gagaagaagc
      121 accgggtcga ggacgccctg tccgccaccc gcgcggccat cgaggaaggc atcgtccccg
      181 gcggcggcat cgccctcatc caggccgccc tggtcctcga gaagtccgac gtggccaagc
      241 tcacggacga cgagaaggtg ggctggaaga tcgtgaagcg cgccctggag gagcccatcc
      301 gccagatcgc cgagaacgcc ggcgtggacg gcgcgatcat cgcggaccag gccaagaagg
      361 aaaagaaggg cgtgggcttc gacgccgcca agatggagtg ggtggacatg accaaggtcg
      421 ggatcatcga ccccgccaag gtgacccggt ccgccctcca gaacgccgcc tccgtggcgg
      481 gcctgcttct gaccaccgag tgcgccatca ccgacctgcc ggagcccaag gcggcccccg
      541 gcggcggcgg tatgggaggc ggcatgggcg gaatgggcgg catgggcggg atggattact
      601 gatcgcggaa gcctcaatcg ccgcatgata gggcgccccg aaaggggcgc ttcttatttc
      661 atgccgccca ttccgtttct gggcatcgcc gaacgccagt tcggcgccgc gaggtcccga
      721 ggggacctcg cgcggcacgc cgtggtcgcg caagcgacca ctacaatctc ttccttgcgg
      781 cgccaagcgc gaagcgcttg atgggcggga tggattacta aaggccggcc aaccagcacg
      841 ctgactagat gggccgcccg caagggcggc cttttcttac aggccgccca ttctggagat
      901 aagctgcgaa gcgggtcgag cgaaagcgag actttttgcg ggcggcacgc cgtggtcgcg
      961 caagcgacca ctacaatctc ttccttgcgg cgccaagcgc gaagcgcttg atgggcggga
     1021 tggactacta gtccgcggac aaccaataag ctgtaaaaag ggccgggccg caaggctcgg
     1081 ttttttaccg ccgccaatga cgctggttaa gttttcaaac accaactcga cgtccttgag
     1141 cgtcggcgtc acgggttcca gtcatggtaa acccgttctc ccgccgtata ccggacggtg
     1201 accctgcgaa ggtagaaatt gctatccgcc ggatccgaac tattatgtcc atgtatgaac
     1261 ggtgcgtaac ccacggaagg agcattgctc gcagtgatcg tggtcgaccg tataaaggta
     1321 tcattcatat agagtgcaat gatattcccg gatcggacca gggtacaagt atttatacca
     1381 atgaccattc cggggacatt cccgtcaata tctgagtatg aataggatcc gttaccctgg
     1441 gaaacttggt agtacgcatc gacatgttcc gggaaagccg tgtaataagc ggcgaaactg
     1501 tagtacttac gggtcgtcga gttttcctaa ttcggatcaa ccagtcgaat tgcgtatcgg
     1561 tagatgtctt cccccgaaag caccttaagg tagaattcga actctgcctc gaaatctccc
     1621 gtgaacagat atggtgcagc aatatctgtt tgattcatgt agaagccttg cgagtattcc
     1681 ccggtattgc ctccgtcgaa gtaccagtcg ctatccagaa ccgcttcccc gtccgtcatg
     1741 aagtcccgct cccacacgac atcacttggt agatcactgc ccggcgcgca acctgcgcag
     1801 acgagggttg agagtaatac aacaaccagg acgtaggaca atctcgatgc catgatctcc
     1861 tcctcgggtc agtaatgggt atccgtcacc gcaaaagcgg cacttggttg aggaaccgta
     1921 cgtttagagg ggattccaag aaaaacggac aagctctcag agggctctga gaaccaagta
     1981 tcgcatcctg cacgggtacg gaaaaggatg atttcacatt ttccctgctt tttggaatcc
     2041 ttgactatag tttcattcgt actcctgatg ctgtcaacga ccggacttcg gcttcttctc
     2101 ggactcatgt ccgatggttt ccggcctccc gttgccgata taccgcgatt ccgcatcaac
     2161 tgaagtcaag tggagctccg ccgagtccag gatgcggcga tgaaagcgcc atctggagga
     2221 ggaatcggct ccctcggcct tgaactcctt gactgtacat tccctgcatg cgaagactct
     2281 ccgaggtgga atacgtagca ttggcgcacc atcctcatag tagggaggct cgacgatgaa
     2341 tgaccacggc gcctataacc ggcactccct ggtacgcgag atgctccaga ctcccgacct
     2401 catccggtcg ttccgcctga aggacgcgga gcccgtgatc gcggccgtcc gggagaccgg
     2461 caaggtgatc ctcacgggcg aaggctccag ccgcatcttc ccggccaaga acgccgtcgc
     2521 ctgggcgcgc aggaaaggac tccgcctcga cctggtcacc gggggcagcc gtcaagcctc
     2581 cgagtacgac ctaggcgacc gggtggtgat gggcgcctcc aacagcggcc ggacccggga
     2641 gctgatcgcc cttttcgaag ccttgaggaa acggggccac aggcggctct actcgatcac
     2701 cgcgaatccg ggtaccccgc tcgagtccct gtccgatcgg actttcctcc tgacctgcgg
     2761 aagggaagac gcggtggcgg ccacgaaaag cgtccaggag atggctctct tctatttcgc
     2821 gctgctgaac gccgcggagg gactcgggat cgacgatcag ctggccgaag ccgccgaggc
     2881 cgtcgagcgc gcactgcgga tgccgataga cggggagatc acggaagccg tggcgggcgc
     2941 gtcgacgctg tacttcgccg gacgcgacga cggggtcgcc gaggagctcg cgcttaagac
     3001 caacgagatc atccgaagga aaagcggtta cttcgaggga acctacgcgc tgcacggcgt
     3061 cgaggaggta ttgaaccccg acgacgtcgt gctttggatc gaacccttcg aggaggaata
     3121 cgagaagatc cggtccgcct tgatcgacgg cgtggggatg aaggtcctgg cgatctccga
     3181 aaaggatacg ccgttcccga cgatccggat acgaggcgcc cgggaactga ccaacctcgt
     3241 ccgcctggcc gcggggtgga acctgctggt cgaggcgggg atgcgtctgg gaatcgacct
     3301 ggaccggccc gcacgggccc ggaaggtcgg taacgagttc accgggtagt ccggctcggc
     3361 tccaagcgtt caccccgcgg ggccgcgagc agccccctcg cgaaatcctc cgccctcgca
     3421 aggtccgcgg cgtccggtct tcccttgttg aggcccccga agtgcttcag gaagctgttc
     3481 gtgttgaacc ccacgcagga gaactccccc gcgatcgcgt agccgcggtc cgccagccgg
     3541 gcccggagct ccgcatgatt ggcctcgacc agccgatcga acgcctcccc ccgggcgaag
     3601 gctgcgggaa ccccgcaggt ggagaagagg aagacccgtc gccccccgcc gggagggagg
     3661 cgatccgcca ggtccaggat gggccggtag tgccggccgg agtcgatccc cgcgccgaag
     3721 ccgaccaggt cgtactcccc gagggtcgcc gggtcgaccc gatcgggcgt ccggacttcc
     3781 gcccccagga ttcgggccag ggcctcggcc accttccggg tgttcccgtg gtgccaggaa
     3841 tggacgatca ccagggcctc ggatccggcc tgccccctca tgccgcgccg cccttcccgg
     3901 ggaccacccc cgggatgccg gcggacgcgc ggaacccctt cacgatcatc cataccgcca
     3961 tgaccatctc ctgcaggaag atcgggaaat ggatccaggc cggcaccgcg gtggctcttc
     4021 cgttcccccg gaacaggtcg aagaatccgg cgcccaaatc cacgaggata gcggccaggc
     4081 cccagacgga tatccatctc ggaacgaggc gcgatcgata gaacgagccg tacaggatca
     4141 gggcgcccag gctgaaggtg aacacggtca tcatccggac cgcttccgaa atccgcagga
     4201 cggcttcccc cgcggcccgg agccccgccc cgtcggcctc cccggcgcgg acgatctggg
     4261 gggccagccc ggccaggacg atccagcagg cggtgatcag gatataggtg acggtctcca
     4321 gggcgccccg gaacaccacg tatccgacgg cggagacctc gctctcccgt ttcaagatcg
     4381 ggtacatcag gatcggcacg aaggccaggg ccagacccat ggtcaggatg gcctgggccc
     4441 ccgcctgcat gcgggggccc agggcggcga tcccggtgaa ggggtccggg gctccgaagc
     4501 cgaaacccgc caggatcacg ctggccaccc ccgaaacggt tcccagtatg tacaggccgc
     4561 cgaccgcaac ggccgtccta cgttcattca tcgttgtcct cctccggtcc cccgggaacg
     4621 tactccaaca ggtccccggg ctggcaggat agctccctgc acagggcgtt cagggtcgat
     4681 acgcgcaccg cccgggcctt cccggtcttc agtatggaaa ggttcgagtc ggtcaagccg
     4741 atccgccggg ccagctcgtt gagcggcatc ttgcggtccg ccatcatccg gtccagccgg
     4801 aatatgatgc tcatctgaag tctccttaca cggtcaggtc gtgctcttcc ttgaggaccg
     4861 ttccggccgc caggatctcg cagatcaaaa gcaggatcac ggccgtcagg aaggatcccg
     4921 cgctgaaggt caggacggcc agggcgatga ccagcttgct ggtccggcgg atgagacgca
     4981 cgttcccggc gtcgaacacg acctcgtcct tgaaattgga gaagagcgac cgggttttcc
     5041 ggagcaccat caggaccagg gtcatgaccg cgccgaagta cggcaagatg aggacgcgga
     5101 ctcccagggg caggctctgg aacctctccc agtcgcggat gtccgaccgg gagctttccg
     5161 ccattccccg ggagaagggg ctgccgagct gttcatggaa gacgatcacc gtgcccagga
     5221 cgatcgcgag ggcggcgcac agccaggcca ggtaccaggc gacggtcagc aagccgtgca
     5281 tgaacgaaga aagggatcgg gtgccgaagt atttcatggg aggacctcct ttggttctcg
     5341 ccatgaatat atcatcccgc tttctgttat tcaagtgtta tttattgttt ttcaataatt
     5401 tttaattatt cgtcaactat acggtattga atttcaacaa taagacaccg cgacgaccgt
     5461 tcaggcgccg aggcggccct cctaccggtc cacccggttg atcatgaggg cctgggatcc
     5521 gtccggacgg gactgctgcc aggtggcgat ataggagtcc ggggcggcca ggacgtccac
     5581 gtagcgcaga cagcctgtcc cgtcggggct cgcgaactcg ggaaagatct cggagagccg
     5641 taggaaggcc gttggatcac catccacgaa ataccccagg ccgcccagtt cctcgcagga
     5701 atagccccgg ggccgccgga ccgcgctcgc gtgctcgtcc aggttgcgca ggcactcccc
     5761 cccgtcgtag aaggccaggc cgagacccgc gggccagtcc gagggggccg tcctcgcgcc
     5821 cgggggcggc ccgggcaggg ggagaacgca ggtgccccgg gtgatggcga cgtcccaggc
     5881 gggtccccgg ggccagcagt cgaagacggg aggtccctcg gcatccccat ccggcccgag
     5941 gggaaggtac ccggtgccgc tggaggacca gttgaaggag tggtggcaga agaagaggta
     6001 ctggccattc ccggggcgct cggccaggaa gggatccttg acctgcaggg tagccggatc
     6061 cgcgccggcc gcgacggtcc ggggccgcgc cccggccaag ccctccagag tcggggcgga
     6121 caggcgctcg atggtccaga tgcccgtgcc gggcttctga aaggcctcga cgggggcggg
     6181 gtacgggacg cgtttttccg aggagacgta gagatccact ctccccgggt cgaagcgcag
     6241 ggcggagccc tcgatcgata gcgcctcccg gccgtccgga cagaccgcgg ccttgtcgaa
     6301 ggacaggacc ttctcccagg accggccgcc gtcccgggag gcgaagaccg cgagttcccg
     6361 gccccggtcc cccaggtcca ggcccgtccg ggaatccccc gcgctgcggt accggcccgt
     6421 caggaagagc cgtccgtcgg ggccgacggt catgttgccg cccccgaacc agtagcccct
     6481 ctcggggcgc tccggaggga tcgccacccg tgcggccgcg ggatcgacca ggcgatgcgc
     6541 gaaggagcgc agatccgaca agactcggga atcgacggga cgggtcatgg gcatctcctc
     6601 gacggcggaa ctttcgcggc gagcgcccgg gattcgcgga cacggcgccg ggtccggccg
     6661 ccgcccacta tagacgtccg tccgggaccc tgtaaacggc cgggacgaac taccaagcct
     6721 cgggaagcag ggggagcctc cgacgtttcc atggcttgac ggacgggccc cagggtttga
     6781 gaatcggggg catgactacg atcgaacgcc tcccccccgt ccgcctctgg atccgcgagt
     6841 ccgggggagc ctggtacggg gtcgccgagt gcgacggccg gctcgcggcc tcgtcgtccg
     6901 ggcggacccg ggaggaggcg gagcgggttc tcacctcctg cattcctccc ggagcgcccc
     6961 ggaccgccgg catcgggccc tcggacttcg cggacgacgc ggcccgcagg ctggccgcgc
     7021 tggaagccgg cggcgagccg gaccggggct tcgagcttgc cccggagtac tacccggaac
     7081 cggccgcctc ggtctaccgc ctggccgcgg ccgtaccccc cgggtacgcc gcctcctacg
     7141 gcggcatcgc ccgggccgcg ggcaccatcg cccgggaggt ggggcgtctc atggcgggca
     7201 accccctcta ccccctggtg ccctgccacc gggtcgtggg ctcggactac gccctggtcg
     7261 ggtaccgggg cgcgacctcg ggaccggacc ttcgggacaa gctcgaacga ctgagatccg
     7321 aggcccgggg cttccgggag gaggtcgagc ttccgggagc cgggggcctc cgggtctttc
     7381 ctgtggagtg ggtcctggcc aaggcggacc tcgaagggga cggcggggga cagccggagc
     7441 tctggtgagg gccgggatcc ccgggattag gccccgtacc ccttctcgat cgtccggatc
     7501 atgtcgaaga gcagggcgtt gaccggagtg gcgaccccgg cctcgcggcc gaggcggacg
     7561 acggtccccg cgaagagctc cacttcggtc ttccggcggg cctccacgtc ctggagcatg
     7621 gaggatttgc ccgaagggga aagcccggcc agggtccggt cccacacccc gatgtcctcc
     7681 tccgtgagcc cggtgcccag aaccttggat atctcgatca gctcccccat ggcggccagc
     7741 atgacgttcc gggcctccgg atactgctgg aatgatgaat acggcgcccg caggatggcg
     7801 gaagccgggt tcatgcccac gttgaccatg agcttgaacc acatcgcgtg gaccatgtcc
     7861 tcgggtacct tgtaccgcac accgcaccgg tcgaagaact ccgcgatccg ggccacccgg
     7921 gggctccacg ccctcggcgg attccggtcc tccccgaagt ggatggtccc gcccacggta
     7981 aagttcgtgg cgttcccctc ccggaccgcg tcgatggcca ggatcatcgc gtccaggacc
     8041 cggtcctccc cgaaggcggc ccggaggtcc ttctcgctgt cgatcccgtt cataagggaa
     8101 agaatgagtg tcccggggcc gacacagggg gccaggtccc ggatcgcggc ggccagctgg
     8161 tggtgcttca cgcagaccag gaccagtccg ggctcgtcgc cgggcgccgg ggcccggccc
     8221 gcggggagga ccttcaggtc caggcgctcc ccgttgacgg aaaggccgtc ccgggccagg
     8281 acctccgctc tccggtcgtc cgcgagcagg cggaccgccc cgggccgggc cttgtggatc
     8341 gcggcggcca ccgccgaacc gatggctccg gcccccgcga ccagggcgta acgtatttcg
     8401 tccactgcgg catctcctga cgaacctgat cttccgggag gcggcggact tcagcccctc
     8461 cccgggttca acataccttc cagcccgtcc aggatcaagg tgaggccgaa ctcgaagtcc
     8521 gcgtcctcgt cgtagccgtg cgcaacggtc cactcgatca tgcgggccag gtgcggatag
     8581 gcttcggtcg gagcggaatc ccgcagcgcc tcggcccggt cctcggcccg ctcgcgcccg
     8641 ccggaggcca tgttgcgcct ctggagcgcg aacccgtaaa tgtagcaatc caggaccgag
     8701 agggcccttc cggcccgctc cagcgggaag cccgcccccg tcaaggcgcc gactacggcg
     8761 tcgaaatacc ccagacgacc ggggcccgag ctcgtgcggg aatccatgag ggcggcggcc
     8821 caggggtgga gggaaaaggc tttccgagcg gagaccgcac gttcccgcat ggccgtccgc
     8881 cagtcgactc cggccgcggg gaccgcgatc tccccgatga cgagttccag aattccgtcc
     8941 aggacgtcct ccttgccctc gacgtggttg tacagggaca tcgcctctac ccccagccgg
     9001 cttcccagcc ggcgcatgga cagggactcg acgccgagtt catccgccat atcgagggcc
     9061 gcgcggagca cacgctccct cgtcaggaaa gccttcccgg gcacgtccac tttcttcatc
     9121 cactcctccc cgcgttattc tactccgtcc ccgggcggat gaattgccca cttgacgaac
     9181 ttacaccgta agtagtaaaa cttacattgt aagtatggcc tttgggccag gacatgtcaa
     9241 gggaacccgc cgggcggggc gaacgcccgc ccggcgagcc gtcccgccaa ccgtccatta
     9301 aaggagtatc ggatgcagga tcttcagaaa gcgggcggca tcgccgccct cgtcgccgcg
     9361 ggaacctacc tgttcgccat cggtctggcg gccaccgtct tgggacccat ggcggacccg
     9421 gacctgggat tccgggagta catgaccttc ttggaagccc acaagaccct ggtgttcctc
     9481 tggcactcgg tcatgtactt cgtcaacggg atctgcctgg ccgtcctggt gccggccctg
     9541 tacgaacgcc tgaagccctc ctccccgcgg ctggccctgg tcgcctcgat cttcggcctc
     9601 ctgtggaccg ccttcgtctt cctgagcggg gtgatcacga accacgggac cgaggccctg
     9661 ctcgccctcc acggcaagaa cccggccggg gccgaggcgc tcaggaccgc cctggacgcc
     9721 gtcaccctgg gtatcgactc ctcggaccgg ctcctcggga gcctctgggt ggggctggca
     9781 agcctggcgg ctttcaaggg aaaaacgttc ccgaggttcc tgtgcgtctt cgggatgctg
     9841 atcggcgcgg ccggcctggt ggggaccgcg atccccgcgg cggtggccgt cgcctacctc
     9901 ttcggggtcg gcgcgatcct ctggtgggcg ggcgcgggag tggttctgat ccggaagcgg
     9961 acctgacggg acttggaatc agaattccga caggatctac aggatggatc aggatgaaca
    10021 ggataaggat ccagatatct catatctgaa actccaatca tgttcatccg gtctcaatcc
    10081 tgttcatcct gtctgatcta ttcagaagct gcctagcctt tcacggcccc catggtcagg
    10141 cccttcacca cgtacttctg gaagatcatg gtcaggatga tcgcgggggc catgatggcc
    10201 accgcggcgg ccatgacggc cccccagtcc acctcggcgt aggacacgaa gttgtagacc
    10261 gcgatgggca gggtcttggt gcgttccatg ctgaggacct gggagaacat gaagttgttc
    10321 caggagaaga tgaaggacag ggttacggcg gtcacgatcc ccggggacga gacgggcagg
    10381 atgatggaca ggaaggcccg ctggcgggtg gccccgtcca ccatcgcgga ctcctccatc
    10441 tccacgggaa tggtctcgaa gtagctggac atgatccaca ccacgatggg cagggcgatg
    10501 agcatgtggg agaggatcag ggcggcgtag ctgtccatga gcttgaggcg ggagaagatg
    10561 atgtaccagg gcatcaggaa ggagatgccg ggcatcagcc gggccaccag gatgagcacg
    10621 gagagtttct tctgcttgaa ccgggcgatc gaatacgccg cgggcaggcc caggagcagg
    10681 gaaaaggcca cggcggtcac gcccacgatg gaggagttga tgaagtactt gaggaagttc
    10741 tgctcctgga agacctttcg atagttctcc aggatcgggc tgaacaggaa ccggggcggc
    10801 caggcgatga tgtccacctg ggtcttgaaa gacgaggcca gcatccagac gaaggggaag
    10861 aggacggggc ccgcgatcaa gaccagcacg aggacgaatc cgagcttccg caggggatgg
    10921 agtttcacgg cagcccccta caactcgaag cggcgccgga gccgcatgat cagggcgctc
    10981 agggtgagca ccaggaggaa gaggaagacc aggaccaccg aggactggcc catccggaaa
    11041 tactcgaagc tgtacttgaa ggccatgacg ttgagggtct ccgatgcgta gcccggtccc
    11101 cctccggtca tggcgtagat gatgtcgaag gttttcagga cgtcgatggc ccggaggatc
    11161 atggcggtca ggagggtcgg cgcgatcatg ggcagggtga tgcggaagaa gatctgcggc
    11221 cccgtggccc cgtccacccg ggcggactcg tagggttccg gcgagagccc cgccaagccc
    11281 gccaggacga tgagggtgat catgggggtc cactgccaga tgtccaccag ggccagggag
    11341 gggatgaccg tggccgcccc ggagacccag gcctggcggg gcaggccgaa gagggacagg
    11401 acgtagttgg ccagcccgat ggtcgggtcg tagaagaggt tccagacgat gccgatggcc
    11461 accggggtgg ccaccagggg caggagcagg atgagcttca tgaagccctt gccccggaac
    11521 ttccggttca ggaggagggc caggcccagt cccaggaggg tttccgcggt cagggccagg
    11581 gcggtgaagg agaaggtccg ccccagggcg tccaaaaaac gcggctcccg aagcacccgg
    11641 aggtaggatc ggaagcccac cagggcgaag ggcatgccgg aggtcaggtt ccagttgctg
    11701 aaggagagga acagggtgta gaggaccggg aagaccatca tcacgacgac gaaggccacc
    11761 gcgggcaggg ggaagaggac gttcaggtgc cgttccagga taccgctctt ccgcatgtcc
    11821 cctccgatca aaaaacagga ctcaccacgg agggcacgtc cggccggcct gagccggacg
    11881 gacggcgccg aagcgagctt cggcgaaata cccggacggg cagatcgacg aagcccgaag
    11941 ggcttcgttg ccgccaggct gtccttagga cagcctggcg cgactccgtg gttaattcct
    12001 ataaacctag tacactccga actcgcctgt gtcctcgagg agttcgttca ccgcccggac
    12061 cttgtccttg gccagctgct ccagcttcgt ggatgtgccc gcggtattga tggactcgat
    12121 gatcacctcg ccgatcaggt cccgggcgtt cccgacggcg ctcatgtagg gccggtcgta
    12181 gggatagccg ttcttggcgg cgaaggcttg ggaggccacg agctcggcgt tggccttggc
    12241 gcggacctcg ggatccgccc aggcggagtt gcgggccatg gtgatgttgg cgatcaggcc
    12301 cttcttggcc agttccttgc tggtcgccca cttgatgaag tccatggcca gggccttgtt
    12361 cttggactgc cgcgccacgg ccatgcccca ggacacgacg atgaaggggc tcgaagcttt
    12421 gggacccgcg gggaggttgg cgatccccac gtccgcggcc ttgacctggc tcttggcggg
    12481 gtcgacgatc tggccgtaga agaccgaggc gtcggtccac atggccacct tgccggcctg
    12541 gaagaggggc atgatgttct cccaggacat ggaggtaacc ccggtgggtc cgtagttggc
    12601 cagcatccgg ccgtagaacc gggtggcgtc cagggcttcc ttgctgtcga acacggcctt
    12661 acccttgtcc aggtagcgcc cgccgtagtt gtagatgtag gaggacagct gggtcaccgc
    12721 cgcggcgccg cgtccgcgcg aggcgaagcc ggcgatgtcc gcggtcttga gggccttggc
    12781 cgccgcctcg agctccgcga aggtcgtggg gaccttgagc ttggccttgg ccaggagatc
    12841 cttccggtag tagaggacct gccactccgt cacgaggggg acgaggtagg gcttggcgcc
    12901 gaagcgggcg atgcccatgg cgctctcggg ataatccgag aagtcgtagc ccgccagggg
    12961 ctcgtaccag ccgttcttgt agaacatctt cccttcctgc aggggccggg tcatgaagac
    13021 gtccacggtg gaggagttcg tggcgaactc cgtggtcagc ttggtggtca gctggctctc
    13081 ctggaggctc tcgacgttca ccttgacgcc ggaggcggcc tcgtactcgg cgatcaccgt
    13141 cttcaaaaga tccccgtagg ggtggttggc caggagcacg cggatctcct tggcctgggc
    13201 ggaaaccgcc atgaccagga cgacggcaag caagatcgcg atcagctttc tcattgcacc
    13261 ctccttgggc tcgatcggac atgaaataca aatcaatcgc attatcaggc cggatttccg
    13321 gaaggtcaag gaaaaaatag aaataatttt ttattgtaag aatttttttt tcaccgtata
    13381 ctggggatgt accatgatgc gtcacacaag ctgcctgtac acgatacacg agcgactcgc
    13441 ctccatgagc gaaaaggagc gcagggtcgc ggacttcatc ctcgactcgc cccaggaggc
    13501 ggtgcatccc agcatcgaag ccctggccga gcgcatcggc gtctccgaat cgactctgtt
    13561 ccgctttgtc cggaagctcg ggtacgaagg ttaccagcag ttccggatcg ccttggccac
    13621 ggagaccgta ggccccaagt cggggtatta cgacgccccc gtcgattcgg aatccggcga
    13681 gtccgtggtg gccgtcgtct tcaaaacggc tatcggcgcc ctggagcgga ccatgggagg
    13741 actcgatccc aaggccgtcg caaaggccgc ggacctgctg acccgcacgc gcttcgtcct
    13801 gttcctgggc ctcggcggtt cggccatcgt ggccaaggac gcctaccaca agctcatgcg
    13861 gacgggcatc cgctgcgccg ctcccgagga cttccatatg cagctcatgg cggcatccca
    13921 gacggaaccc ggcgacgtcg cccttctcgt atcccacacg ggggccaaca aggacgccct
    13981 ggcgatcgcg gacgtgctcc ggagccgcgg cgccgtcatc gtcgtcgtga gttcctatcc
    14041 tcggactccc ctctccaaga tggccgacgt gctactcctc tccgccgcgc ccccctcccc
    14101 ctattccatc gaagccttct ccgcccgcct ggcccagctt gccctggtgg acgccctcta
    14161 cgtcgagatg atgcagcgcc ggggcgagga gggatcccag gccctgggag agatgagatc
    14221 cgtcatcgcc aagcgcagga cataatcgca cgcctcccgg gggcggcccc gctgcctccg
    14281 cggggcatcg acggacggga acatgcggaa cggggccggc ttcatcccgt cgaggaccgg
    14341 cccgaaagga tcgcccatga aagccctggt actggaagaa tacaaacgtt ttgcgtacgt
    14401 cgacgtcccc gacccggacc tgcggggacc gaaggacgtg atcgtgcggg tcagggccgc
    14461 ggccatctgc ggctccgacg tacacgggat ggacgggtcc acgggccgcc gacgccctcc
    14521 ggtcatcatg ggccacgagg ccgcgggtga gatcgtccgg accgggaccg aggtccggcg
    14581 cttcaaaccc ggggaccggg tcaccttcga ctccaccgag tactgcgggg aatgctggca
    14641 ctgccgccgg ggcgaggtga atctctgcga cgaccgccgg gtcctgggtg tctcctgcga
    14701 ggagtaccga agggacgggg ccttcgcgga atacgtggcc gtgccggagc gcatcctcta
    14761 ccccctgccc gagggactgt ccttcgtacg ggcttccctg acggagcccc tggccgtggc
    14821 cgcccatgcg gccatgctct cgccccccct gcccggggag tccgcggcgg tggtgggcgc
    14881 ggggctcatc ggcctattgt tgatccaggt cctgcgcacg tccacctccg gccccctctt
    14941 cgccctggac acggaccccg accgccgcgc ccaggccctg aggctgggag cggactacgc
    15001 cctggacccc gcggacccgg gggccctgga ggagctccgg tcccggtgcg gagggcgggg
    15061 cgccgacaag gcctgggaag ccgtggggat cacctccgcg gtggccaccg ccgtggattg
    15121 cctccgcaag ggcggcgccc tcaccctgat cggcaacgcc agcccccgga tcgacctggc
    15181 cctccagaag gtcgtgaccc gccagctgac cctgcgggga agctgcgcca tagccgggga
    15241 gtaccccctg gccctggacc tcatggcccg ggggcaggtg gacgtggacg ccctgatctc
    15301 cgccacggcc cccctctcgg aaggagccgc ctggttcaac cgcctctacg cccgggagcc
    15361 cggcctcctc aaggtcgtcc tggaaccggg gcgctaggag aaacccatgg aagagctgcg
    15421 tttcgccctc tacgggtacg gcaaggtggc cgaactccat gccaaggccc tggcctccct
    15481 ggcgggaacc gcccggctgg tgtccgtgat aggccgggac aagggaaagc gggaagcctt
    15541 cgccgcgcgc tggggggtct ccgcccgggc gaccgccgcg gaggccgcgg acctcgacca
    15601 tgcccgggcc gtcctggtca cgacccccca tcccctccac gccgcccact cccgggaatc
    15661 cagcgccgcg gggctccacg tcctcgtgga gaaacccatg gccctgtccg tggcggactg
    15721 cgacgccatg atcgccgcgg cggccgctgc gggcagggcc ctgggggtgg tgagccagag
    15781 gcgctggtac cccgcggtac gccggatccg ggaagccctg gactcgggcc gcctaggcac
    15841 ccccgccctg ggccaggtgg tcatgctggg ctggcgggac gaggcctact atcgctcgga
    15901 tccctggcgg ggttcctggg ccggagaggg gggcggggtc ctggtgaacc aagcgcccca
    15961 ccagctggac ctgctctcgt ggttcctggg tcccgtggtg gaggtgtccg cctactgggc
    16021 caacctcaac cacccctaca tcgaggtgga ggacacggcg gtggcggccc tccgcttcac
    16081 cgggggggcc ctgggctcca tccttgtgtc gaactcccag aaacccggga tctacgccaa
    16141 ggtccacgtc cacggatcga acggagcttc cgcgggggtc cagaccgacg ggggggccat
    16201 gttcatcgca ggcatgagcg gggtcctgga gcccccggtc aacgacctgt ggaccgtccc
    16261 gggggaggag ggcaagctgg ccttctggaa ggccgaggac gaggcgttct tcaagaccgt
    16321 ggacgccacc tggtggttct tcgcccggca ggtcgaggac ttcgccgccg ccgtccggga
    16381 gggccgccca cccgcggttt cggggcgcga cggacgcgag actgtgcgtc tgatggaagg
    16441 gatctacggg tccgggagga gcggcgggcc ggtgcggccg gcgccgtaaa ccgaaggctc
    16501 gctcggccgg accgggtcaa ccggccgggc ggcccgtctc gagcactacg aggtgctgga
    16561 agtggtccac cacgtcgtat cggtccacct cgatccgcac cagggccgtg tccggggcgt
    16621 cggcgaactc cgccagctcc gggtacttgg ccaggtagag ctccttgtac gccccccgat
    16681 ccggctccgc aagctcccgc gccgaacccg aggcctcgac gccggtgacc agcatcaggt
    16741 cgtccatgcg gtccctccgg tcgtcggtga acagggaaac ctcgggacgg tcgcgcaggt
    16801 accggtactt tcgggtgctc cgggaagtgg cgaagataat gccgcccagg tcgtcgacgg
    16861 aaacgaagga gacgatggaa aggtggggcc ggccgtcctc accccgggtg gcgaggacgc
    16921 cgaagagctg gcgcgccagg aggcgtctga ccgaaattcg aagatcctcg tccgtcatat
    16981 ccgctccccg aaaccgctta gtcgggcgcc gattcggcgt ggtaatcgtg cagttcggtg
    17041 gaccttcggg tccaccgaac tgcgaaacca aaccccggac cgctagaagc ggtccggggt
    17101 ttggttagac ttcatacgtc cccgccgcca ccttgcctcc cgtgccggtc cagtcggtgt
    17161 ggaaaaactt gccccggggc gcgtcggtcc tctcgtagga gtgggctccg aagtagtccc
    17221 gctgggcctg gaggaggttt gccgggagcc gctcggagcg gtagccgtcg tagaatccca
    17281 gggccgtgga cagggccggg acggggatgc ccgcctccac ggccttggcc accacccggc
    17341 gccaggatcc ctggcagcgc tcgatcacgc tccagaagta cggatccagc agcagattcc
    17401 tgagattcgg gtcccggtcg aaggcctcct tgatcctgcc caggaagacg ctgcggatga
    17461 tgcagccccc tcgccacatg agggcgatgc cgccgtagtt gaggttccag ccctcctcct
    17521 tggcggcctc ccgcatgagc atgtagccct gggcgtagct gacgatcttg gacgccagga
    17581 gggcctgccg gaggtcctcc aggaaggcct tggcgtcggg gacggagatc ttcccggaac
    17641 cgggcccggc gaggaccttg gccgcctcga cccgctcctc cttcatggcc gaaagacaac
    17701 gggcgtacac ggcctccccg atcagggtga ccgggacccc cagctccagg gagctgatac
    17761 ccgtccactt gcccgtgcct ttctggcccg ccgtgtccag gatcctctcc accagggggg
    17821 agccgtccgc gtccttgtag gccaggatgt cccgggtgat ctcgatcagg taggagtcca
    17881 gctcgccttt gttccattcc gcgaagacct cgtgcatccg ggcggcgtcc aggcccaggc
    17941 cctgcttcat gacgtggtag gcctcgcaga tcagctgcat gtccccgtac tcgatcccgt
    18001 tgtggaccat cttcacgaag tgcccggccc cctcctggcc gacccagtcg cagcagggct
    18061 ggccgtccgc ggtcttggcc gcgatggcct ggaggatgtc cttgaccagg ggccaggcct
    18121 cgggattgcc cccgggcatg atggagggcc cgtgccgggc gccctcctcg ccgccggaga
    18181 ccccggtgcc gatgaagcgg atgcccttct gggccaggta gcgggtgcgg cgggtggagt
    18241 ccgggaagtg ggagttgccg ccgtcgatga tcacgtcccc ggcggccagg tgcggcagga
    18301 gggtctcgat gaactcgtcc accgcggcgc cggccttgac catcagcatg atcttccgcg
    18361 gccgcttgag gagggcgcag aactcctcca cggaacgggc cccgtagatc gtggaccgcc
    18421 ctttggccgc gtccgccagg aactcgtcca ccttggagac cgtgcggttg tacaccgccg
    18481 ccgagtaccc gtggtcgttc atgttcagca ccaggttctg gcccatcacc gcgagtccga
    18541 tgagcccgat gtccgccttg gcttccatat gtattccgtc tccttcaacg ttgtcttgag
    18601 gcgtcacgag gcgaagcggc cgcggtacgc ccagaggccc acggccggat tgctgatgta
    18661 gaagatctcc tcgccgtccg ggagggtgtt gaagccctcc atgaggcggg cggggtcgta
    18721 gcgcttgagg gtttccccga gatccgcgaa gcggaacccg accccctcca cctcctcccg
    18781 ggagaggtgc ccgggggcgt aggtgatgga gaagcggccc tccgaggagc cgtggatcag
    18841 gtgggccgcc gcggagaggc tggccttgag gtccgcctcc ttttccacca ggcccaccac
    18901 ccggtccgtg ccggagtagc cgtacttgcg gatcagccgg tcgatctcgg gatcctcccc
    18961 gaactccttg acccccggtg ccaggacgac cagctccccg ccgtccgcca gggccatccg
    19021 ggtccggtac acgctcttgt tgcccagcca ggtgctccgg aactccttgg ggtccaggta
    19081 taccaccgcc ttggccaggg gcttgtccag catggtgaag ttaaccttca gggagagctc
    19141 cgccgctttc aggaaacact ccaggtcgtc cccgatatag aggccctgga cggccaggga
    19201 cccgtccgcc cgccggccca ccacggtgtg ggcgaagacc agggggaggt gccgggcgaa
    19261 ccggtcgtgg gcgtagttga ggaccttccg gaccgggttg atcgcccgcc ccatgatgcg
    19321 ctccatgccg tagaccgcgc ccaggtagtg gctcttgttg atcccctcgg ctccgccggt
    19381 gcccacgaag acgttcttgt tgtagttggc catgccgatg acctcgtggg gaaccacctg
    19441 tcccggggac aggatgaggt catgcccgcc ttccacgacc atgcggttgg cctgggccgg
    19501 ccaggggaag gagagctttc cctcggaaac ctcctcgatg aaggaggcgg gcacctcccc
    19561 cagggtggcc acgtccgtgc gccagcggtg cacccggaag aggttttcgg gcacccccgg
    19621 gaacatctcc cggatctcct cgcttgtcat gggcttgtgg gtgcccaggg ccggcaggat
    19681 gtccgtcagg gcggatccgt agcggtcgta ggcgaagcgg gtcaggatcc ccgcccggct
    19741 gtggacccgg gtgatgtccg ggggcaggac caggacccgc ttgggacgcc cgcgggcggc
    19801 ctcgaccttg tcgagcccct cctggaaggc ggcttccagc tcccggtccg taaaggcggt
    19861 gtccggcgag cccttggcgt agtacagcat ctatcgtccc ttccagggcg gaagctcggg
    19921 gaggtagcgg tcgaactccg cgatgagggc gctctcgtac tccccgccgt aggtccccgc
    19981 gaagaagcgc cccagggctt cctcgtggaa gagcttccag gaggcctcct cgtgcccggg
    20041 attcaccggg aagaagaggg ccccgttctt cttggccgcc tccaggtccc ccggggcgtc
    20101 cccgatcatc aggacatgcc cgggggcgta cttgcccttg gacgcgaagg cgatgtgctc
    20161 ggtcttggtg cccatctcct ggcccgcgat gacccggagg tggcccgcga tgccgtgctc
    20221 ctcccactct cgctggaggg cctcctgggg ggtctgggac acgacgatga cgtccgccct
    20281 ctcctccatg gccgcgaggc tctcccgcac gagggggaag ggcggaacgt cccggacgat
    20341 cctggccacc gtctcgttga cgtccacgga ccaggcgtag accctccgga ggtcgggatc
    20401 cccggtccgg tcgagctcgg ccttcagggt cgggtttccc agcttggtct cccgcctcac
    20461 ccagtcccgg aggcccgcca gttcggggac cctcaccccc cgggccttgg tctcgtcccg
    20521 ttcccggagg aggtccaggg ccctgagcac cgcggggaag cggttgcagc cccgggtctt
    20581 ggaatagagg ttcacgaaat cccaggcttc ccgggcgtac ttggaaaccg cctggaggcc
    20641 gtagtggttg acgaaggcgg gacagaagca ttccttgtgc ttgatctcca tggaatcgaa
    20701 gacgcagccg tccgagtcga tgccgatgaa gaactcgtgc tcgggcttca ggtcccgcag
    20761 gacctgggag ggatcgttcg tggttgcgct catcgggctt acctttcctt tcgaaatatc
    20821 atccgatacg aaatcagata ccacaaacac cctccgcagt caagggaagg cgcggcggtt
    20881 gacagcacgg gggtttcatt atactctgcc attcgaaagg taacgaaaga tgacccacct
    20941 gccccggcac gccgagatcc tgaagatcct cagcctcctc cggaacgtct ccgtggggga
    21001 cttcgcccag cgcctggggg tgtcggaggt gacgatccgg aaggacctgg cccagctgga
    21061 ggagatggga tgcctgatcc gcacccgggg aggcgcccag atcgccgagg atacccgggt
    21121 cctgaagacc atggacgtgc ggctcaagga acgcatggac gtgaaggagg ccatcgcccg
    21181 gaaggcccgg gagctcgtcc gggacgggga gaccgtctac ctggacgccg ggagcacctg
    21241 cctcctcctg gccaaggagc tcacggagat gaacctccgg gtggtgacca actccatcga
    21301 cgtcatggcc gccctgggag cctgcccggg catctccctc atcagcctcg gaggcacgta
    21361 ccgccgggag gcgggatcct tcgtcggccc catggccatc gaagccctcc ggactctcca
    21421 gatcgagacc tgcttcctgg gcgccacggg cttctcgaac cgcggggctt tctcgtccca
    21481 gaacctcatc gagtcccagc tcaagcagaa ggtcctggag gcctcggggc gacggatcat
    21541 cctggcggat tccggcaagt acgggcgcca ggccttctcc gtcttcgccc gggccgggga
    21601 cgtggacatc ctggtgacgg acccggggtt ccggaacatc aaggagttca acgtcttcgg
    21661 catggagatc gttctcgcga ccgggatcgc cgcaagcgat cccagtcgcg gccccgaatc
    21721 cggctcaagt gccggattcg gcgagttcca ggctaggaaa accgcgggaa tcggcgaatg
    21781 acaggacttg gtacaacgcg ggaccggcgc gtatgccgcg gacccgaatc cgcatcagga
    21841 ggaatccatc catgaagggg aactgcgtcg tagcacagag cggagggccc acggcggtca
    21901 tcaattccag cgcctgcggg gtgatccagg aggccctgaa gagcccggac atcaccgggg
    21961 tgtacgccgc ccacaacggc atcctgggca tcctgaatga gaccctcttc gacctgggca
    22021 aggaggatcc cgcggacatc gagggcctca agacaacccc ctccgcggcc ctgggctcct
    22081 gccggtacaa ggtgaagacc gaggaggact acggcaagat cctggcggtc ttcaagaagt
    22141 tcggggtccg ctatttcttc tatataggcg gcaacgactc catggacacc gcggacaagg
    22201 tgaaccgcct ggcctctcgg gagggctacg agttccgcgc catgggagtg cccaagacca
    22261 tcgacaacga cctggccttc acggaccact gccccggcta cggcagcgtg atcaagtacc
    22321 tggcgaccat ggtcatggag gcgggccggg acaccgaggc cctctatacg gcggacacgg
    22381 tgaacgtgat cgaggcgatg ggccggaacg ccggctggat cgccgcgggc acggccctgg
    22441 ccaaacggag cgaggaggac gcaccccaca tcatcctgct tccggaggtc cccttcgatc
    22501 ccgaagcctt caaggaccgg gtgaaccact acctcaagac gatcaagcgc tgcgtcatcg
    22561 tcgtgtccga gggtaccaag aatcccgacg gcacctacat ctccgagcag aaaggccagt
    22621 tcgccaagga ctccttcggc cacgtccagc tgggcggggc ggccaactac atcaaggccc
    22681 tggtggaagc cgaggtcaag gtgaaggccc gcttcgccat cccctccacc atccagcggg
    22741 cgggagccca cttcgccagc ctcacggact cccaggaggc ctacgagacg gggcgccagg
    22801 cggtccgcct ggccgtggcc ggggagagcg gaaagatggt caccctggtc cgggattccg
    22861 acgaccccta ccgctggagc acgggcctgg cggacctgtc ccaggtagcc aacggggaga
    22921 agtacttccc caaggagtgg atcggggccg acgggttctc cgtgaccgac gccttcctgc
    22981 gctacgcccg gcccctgatc cagggggagg tcccggtccc catcgagggc ggcctgcccc
    23041 ggtacgtccg gttcaacaag cacttcgtga aaagcgtctg aagcggcgcg gtccggacgg
    23101 cggatcccga cgcggacccg gccgggccgg gttcatatgg acggagaaac gaacagcccg
    23161 ccgatgcccc gcaacggcgg atacccgaga acgaggagac agcgatgact tacctggaag
    23221 agatgttttc cctgagcggc aagaccgtgg ccatcaccgg gggcggcggc gtgatcgccg
    23281 gcgccatgtc cgaggcattc ctgcgggcgg gcgccacggt ggccctctgg gacatcaagc
    23341 tggagttcgc cgaggcggcc cggcagaggc ttgcggccct gcccggcctt tcggacgccg
    23401 cggaccgcat cctgcccgtg gaggtggacg ccctctcgga gccgtcggtg cgcaaggccc
    23461 tggaggccac cgccgacaag ctcggcgggc cccacgtcct ggtgaacgcc gcggggggga
    23521 acaagggcaa gtccgccttc ctggagatcg acgtccccct cttcgaggac atcctccggc
    23581 tgaacctggt ggcgggcctc gtggtcccca ccaaggtgac cgccgccttc tggatcgaga
    23641 aggggatcaa ggggaacatc ctcaacatgg cttccatggg ttcctacatc cccctgtccg
    23701 gggtctgggc ctacgacgcc gccaaggcgg gggtcatgaa cctgaccatg gcctgcgcca
    23761 aggagttcgc cccgcacgga atccgggtca acgccatcgc cccgggcttc ttcctcggca
    23821 accagaaccg ggccctcctg gtggacgaga agaccgggga cctcactccc cggggcaagg
    23881 acgtcatcgc ccacaccccc ttcggccgct tcggggacgt gtccgagatc gcgggaatca
    23941 gcctctacct agcctccgac aaggcggcgg gcttcgtgac cgggatcacc gtgcccgtgg
    24001 acggcggcta cctggtccag aacatctgac atggcgagct tcatccacga ggacttcctc
    24061 ctttcgggag agaacgcccg ggccctcttc cacgaccacg ccgagggcat gccgatcttc
    24121 gactaccact gccacgttcc cccggcccag atctccgaga acacccgctt cgagaacctc
    24181 acccgggtct ggctgggagg agaccactac aagtggcggg cgatgcggac cgcgggggtc
    24241 cccgagaacc tgatcaccgg ggacgccccg gaccgggaga agttccaggc ctgggcccgg
    24301 accgtgcctc ggaccctggg caaccccctc taccactgga cccacctgga gctcaagcgg
    24361 gtcttcggga tcgacgaact cctggacgaa cagtccgcgg accggatcta cgaccgatgc
    24421 accgccctcc tcgcccggga ggactaccgg gtccggggcc tggtgcggcg cttcggggtg
    24481 gccgggatct gcaccaccga cgacccgacg gataccctgg agcatcacgc ggccatagcc
    24541 gcggacgaat ccttcccggt gaaggtccta cccgccttcc ggcccgacaa ggcccacgcc
    24601 ctggagagcc ccgagcgctg gaaggcctgg atcgaggccc tggaagccgc ctccggcgtc
    24661 ccggtcacgg actacccctc cctggtcgcg gccctgaaga accggcacga cttcttccac
    24721 gcccggggct gccggatctc ggaccacggc ctgcagttcg tgcccgcccc ctcggcgtcc
    24781 cccgcggacc gggaccggat cctcaaggaa gcgaaagccg gccgccggcc ctcggacgcc
    24841 gacatcctgg cattccgggg cgccctgctc acggacctgg ggcgcatgga cgccgaggcg
    24901 ggctggacga tgcagctcca cgtgggggcc ctgcggaacg tcaacccccg catgttcgcc
    24961 tccctgggcc cggacacggg ctacgacgtc atcggggact ggccccaggc ggagggcctg
    25021 gcccggctcc ttgcagacct ggaccaagac gaccgcctgc ccaagaccat cctctacgtc
    25081 ctcaaccccc gggacaacga ggtcctggcc acgatggccg gggccttcca ggacggccgg
    25141 accgccggaa agatccagtt cggctcgggc tggtggttca acgaccagaa ggacgggatg
    25201 gagcggcaga tgaccgccct ttccagcatg ggacttctgt cggcattcgt ggggatgctc
    25261 acggattccc ggagcttcct gtcctacccc cggcacgagt acttccgcag aattctctgc
    25321 aacctcgtgg gcggctggat ggaccggggg gaaatccccc cggacctcga ggcggcgggg
    25381 aacctcgtga aggacgtctg cttccggaac gcggcctcgt acttcggcat cgacctgggg
    25441 gtcaaagggt aatccccgga cagcgtccgc agccgcgggg cgcacactac aaaggagagt
    25501 ccatccatca tggaacggaa cgatttgagg atacaacgga accgcgagct cctccttgcg
    25561 gccctggacc ggcagggctt gccggacgcg aagcccgaag ccctggccgc ccatcgggag
    25621 cggtccttcg gccgcacgga cctggggggc ggggttttct actacccgag atccctcttc
    25681 gcggcgggga gcctccgctt cctcctggcg ggcacgggga tcccggccgc cccggacgcc
    25741 ccgatgaacg agcgggacgg caagcgcctc ctcgtcctgt ccaaggacgc gattcccccg
    25801 gccttcgaag gcgagcgctt ctccgcagcg gggtggaagg tcctggacgg tccgcggtcc
    25861 tggggcaact accaggccat caaggagcac ctgcccttcc ttgcccccac ctccctccgg
    25921 ggcgagcgca ccaccatcgg ctgcggggac cgactgggca tggccgggcc cggacatatc
    25981 cgggcggccc ggggtttccg ggtcagcccc gtcctggccc agctgtccat gcgggagctc
    26041 aagctcaccg gccggaccta cccccgggtg gtcaaggacg ccgccttcgc ggccctccag
    26101 gagggcttcg accggggctt cggggccgac ggggaccacc tcaagaacat cccggacatc
    26161 gacacggccc tggacgccgg catgcccatg atcaccctgg accttacgga agtcatggac
    26221 cccgcccccg cctcctggac cgaggccgcg gtggaggagg ccttcggccg cctggcggag
    26281 cccgtccgcc gccgcgtcct ggacggctac gccggaaaga ccttcgccct cgcgggcgcc
    26341 tccatcgtcg tctcggacct cgaggccaag cgctgcgccc tgatgtactg gaaggccctg
    26401 gagttcaccg gggaggtgga ccgccacctc cggtcccgcc ggggggacgc ctacgacctg
    26461 gaggtgagca tcgacgagac cacggctccc acggtgcccg cccaccacct cttcatcgcc
    26521 tccgaactgg cccggcggga ggtgacggtc aactccctgg ccccccggtt catcggggag
    26581 ttccagaagg ggatcgacta catcgggaac cccggggact tcgagcggca gttcgcggtc
    26641 cactgcgcga tcgccaaggc ctacgggggc tacaagatct ccatccactc gggaagcgac
    26701 aagttcagcg tgtacccggc catcggccgg cacacgggcc tgcgggtcca cgtgaagacc
    26761 gcgggcacca gctggctgga ggcggtccat gccgcgtccc tccaggaccc cggcctcttc
    26821 cgggacctcc tttccaaggc ctacgagtac ttccccgacg ccctgaagct ctaccacatc
    26881 accccggacc tctcccggat cccggacccg aagggggtcg cggacgagga tctcccgggt
    26941 tacctcgagc ggcccgagag ccgccagctc ctccacgtga gctacggggg cctcctgaac
    27001 gacccggaca cggggccccg gctcttccgc ttccttatcg accgggagga gctccactac
    27061 ggctgcgtgg gggaccacat ccacgctcac atcgaacggc tgggggcacc gccgcatggg
    27121 cgatagggag gacaccctcc ggttcctgga tttcgacggg gtcctctgcg acagcctccc
    27181 ggagtgctac gccgtgtccc ggaccgccta ctggaccggc ttcctggggc tgccggagcc
    27241 cccggcgtcc gtcccggagg aggccgagtt cgctcgcatg cggcccctca tccgccgggg
    27301 cgcggactac ctcctcctgc acctggccat gagggagggt ctccgcctgg tctcccaggg
    27361 ggacttcgac gccctggcgg cccgggaggc ggagcgggac ggcctcttcc acgacctctt
    27421 ctaccaggcc cggagcgatc ttctgcggcg cgacccggaa cgctggttcg ccctgaaccc
    27481 cctgtatccg ggaatccggg aaatcctgtt gcggaatcgc gcgaaccgtg ctgtccttat
    27541 cctgtccacc aaggaggctc ccttcatcct ggcgatcctc cggcaccacg gggtcgagtg
    27601 ggaggcggac cgggtcctgt gttccggcaa ggaatccaag ctccggttca tcgaccggat
    27661 cctctcggag cggggcgccc ggggggcggt cttcgtggac gaccaggtag accatttcca
    27721 gggcccgtcg gagcacccgg tccgccgcgt cctggcggat tgggggtacg tacggcccga
    27781 gtggatcgag tcggggacat tcgaaacggt ctcgctgagc cggctgccgg aacttctgcc
    27841 ggcctaggcg cggccgcggg ccccgcgccg ggcattcccg ggaaaccggg aatccggatc
    27901 gggatccggg ccaagctcct ggccttttac ggggcggcca tcctggcggt ggtgctcctg
    27961 gacctctcgg tccaggtggt ctccttcggg gccatccgcc agttcgaggg caacctggcc
    28021 cggtaccacg ccatccaccg tctgcgcgcg ggactgtccg cccagtacgc ccgggtggaa
    28081 cgccagatgc gggagtcccc cgacgcgggc tccctgtccc tggagcagga tctccaggac
    28141 ttcctgatgg tcatccagca cctggaggag acggaacccg agtccctgga ggccttcttc
    28201 gccctgcagg cgacccaccg gggcctgccg gcctacttcg agaagctgga agccgcgata
    28261 cgccgccgcc tggccaacga ccgggactgg taccaggaca tggcctgggc cggtcggatc
    28321 gcgggctata tggacggcta cctgtccacc ttcctctccg aggccatgca gtacggggac
    28381 gcccggtacc aggccctgac ccggaggctc aactccctgc gcaggctgac cctcgggggc
    28441 ctcgtcgtct tcgtggtcct cttcggcatg gcggcggtgg ccatctcctc ctccgtctcc
    28501 gcccccatcc gccggctggc ccgcgccagc gagcggatcg ccgcggggga gctggacgtg
    28561 cccgaggtcc gggccgacac gggggacgag gtggagatcc tggcccggtc cttcaacacc
    28621 atgagcaaga gcatcgcctc catggtcgtg gacctccggg gcaaggccga gctggaacgg
    28681 agcctccggg aggaggagcg ccagctcatg gacaaggagc gggccctccg ggaggcccag
    28741 ttcatcagcc tacaggacca gatccagccc cacttcctct tcaacgccct gaacacgatc
    28801 gcccgcaccg ccctgctgga aggagcgaaa cggaccgagg ccctgtccct ggccctgggg
    28861 cgcctgttcc gctacgccct gggcgccccg gaatccatgg tcaccctccg cgaggagctg
    28921 gccatcgtgg aggagtacct ggccttccag cgcctccgct tcggggagcg cctccggtgg
    28981 accgtggagg ccgccccgga gctccaggac gtccggatcc cccggttcac gatccagccc
    29041 ttcgtggaga acgcggtccg ccacggcatc gagccccggg aggaaggggg aagcgtcgag
    29101 atctccgcgc ggatccgcaa gggccgtctg ctcctggaca tccgggacac cggggtgggg
    29161 atggccgcgg cgggacgagg atcctccggc ggaaggtctc ccggccgggc cggggagggc
    29221 atcgggatct ccaacgtgcg gaaacgcctg gagctgcgct acggcccggc cgcccgcctc
    29281 acggtccgga gcgagcgggg acagggcacc gcggtccgca tcgggatccc cgcggaacgg
    29341 gacggggggg ccccgtcgtg agcttccgca tcctgatcgc cgacgacgag cagctggagc
    29401 ggcaggccct ggcccacatc ctgggcggac tttccgaccg gggcgtcgag ctggtcgaag
    29461 ccgccaacgg gcgccaggcc gtggcggcgg cctccggggg aggaatcgac ctggccttcc
    29521 tggacatccg gatgcccggc atggacggcc tggaggcggc tcggatcctc cgggacgccg
    29581 accccggtat ccacatcgtc ttcgtgaccg ccttcgacaa gttcgactac gcccgggagg
    29641 ccctgcggct gggggtggac gagtacctgg tcaagcccgc ggccgcggac gaggtgctgg
    29701 ccatggcccg gcgaatcctg gaccgcctgg agcgggagcg gctcgggccg ggggcttcct
    29761 ccgacggagc tctgtccctg ctggaggacg agctccgcgc cgaccttggt cgggaccttg
    29821 gtcgggacct tggtcgggac cttggtcggg acgcggaccg ggacgccgag atccgggagc
    29881 ggctggccgc cttcctccgg ctccggggct gccagggccg gatcgcggcg gccctggtgc
    29941 tccggcccgc gggcccggcc gccccggaac ccggcccctt cccccggcgc ccgatcggcc
    30001 ggggtacccg ggagctcctg gagcggcgcc tccgggaggc cgggtggtac gccctggcgg
    30061 gggaggggga gcccgaactc cgctgcctgg cggtcgcccc ctcctccgaa ggcgcggagg
    30121 cggaggcgga ggaagccctg tgggccgtac tggcggcctt gacggaggag ctccgccggt
    30181 ccctgggaat ccgggtcctg accggggcct gcctctcccc cgacgggtct ccccgtttcc
    30241 gggaggcccg ccaagccgcg gctttagccc ggatcgaccg gcccgtggtg atcctgacgg
    30301 acgacccggg tcctcacgcg gcctccgagg acgcggacgg ccccggagcc ggggccgtcg
    30361 agcgggccgt cgactacctg cgggagcatc tggccgagaa cctttccctg gtcgacgtgg
    30421 cccgggccgt aggcaccgcc ccctcccacc tctcccgcct cttccgccgc caccgggggg
    30481 aaaccttcgt ccaggtcttc tgccggctcc gcgtcgacgc ggccaagcgc ctgctccgga
    30541 ccggcgccta ccgggtcaag gagaccgcgt ccctggtggg gttcaaggac accgcctatt
    30601 tctgccgggt cttccgcaag tacgaggggg ccagcccctc ggagtaccag gccggctccg
    30661 gataggccgc cggacccgcg gaaccgcccg aggtgtcaag aaagtacccc attacgcaag
    30721 aatttcccca acccggatcc gcggaacggc cctatcctgg atcatcgttc gacaagtttc
    30781 gacataggag gaacccatga gccttaagaa agcgttgctt ctggcgctga tcgtggtcct
    30841 ggcggtgacg tcggtggccg cgcagaagaa gcccatcgtc ctgcggctgg cggaaaccca
    30901 tcccgccgac taccccacca ccctggggga ccagaagttc gcggagctgg tcaaggaacg
    30961 gtccaagggc cgtatcgaga tccaggtcta ccccggctcc cagctgggcc aggaacgcgc
    31021 cgtgatcgag caggtgcagt tcggcgccat cgacttcacc cgggtctcca tctccccgct
    31081 ggcctccttc gtcccggtct tcaacgccct ccagcttccc tacctgtacc gctcggaaga
    31141 ccacatgtgg aaggtcctcc tcggcccgat cggcaaggag ctcttcgcct ccctggagcc
    31201 ctcgggcttc atcgggatcg gctggttcga gcccggcgcc cgcaacttct acaactccaa
    31261 gcgccccgtc tccaagcccg aggacctcaa gggcctcaag atccgggtcc aggagagcga
    31321 gctgatggtc ggcatggtcc agggcctggg cgccaccgcg accccgatgc cctacggaga
    31381 ggtctactcg ggcctccaga ccggcgtcat cgacggcgcg gagaacaact ggccctccta
    31441 cgagtccacc agccactacg aggtggccaa gttctacacc ctggatgagc acacccgggt
    31501 accggagatc atcatcggct ccaagatctc cctttccaag ctctccccga aggacctcga
    31561 gctgatcaag caggccgccc tggacgccat cccctaccag cgcaagctgt gggccgagcg
    31621 cgagaaggcc tcggaggcca aggtccgcgc cgcgggtaac accatcaccg tgatcacgga
    31681 caaggcgccc tggcaggcgg ccatgaagcc cctgtacgac aagcagcccg cggccatccg
    31741 gtacctggtc cagcggatca gcgaagtaaa gtagcaagac gtctccgaag cggaagcggg
    31801 cggacggccg accgggcgcc cgcccgtttc catgcccggg gaaaggcgga accatggcac
    31861 gtagaatcgt agccttcttc aatctcctgc acatagcgct cctgaccctg gcgaagttca
    31921 tgatggtggc catggtcctc gtggtcttcg tgaacgtggt gctgaggtac ggattcaaca
    31981 gcgggctcat gtggtccgag gaggtcgccc tgctcctggc ggtctggttc ggcttcatca
    32041 gcctggccct gggcgtcaag cagaacctac acatcaacct gaccctgatc tcggagcatc
    32101 gtatgtccga ccgggccaac cgggtcctgg atatcctgag ggggctcgtt tacctcgcga
    32161 tcgggctggc catgctggtg ttcgggtgga agctctcgaa gatgaccatg ggttccatca
    32221 tgcccgcaac cctctggccc gcgggaatac tctacgcggt gcttcccctg agcggcttcg
    32281 tcgtcatcta tgaggccgct gcggacctgg ccgggatcga cacccgggac gcggccgtgg
    32341 acaagtacct gtccggagag ggcaccctcc gggacgcgac gggaggaccc gatgcctgat
    32401 ttcaccttcg ccgtgatcta cctgatcgga tccttcctgg tcctgctcct gctccgggtc
    32461 ccgatctcct tcgccctcgc cttctcctcc ctgagcacgg cggccctcct cgggatcccc
    32521 ctgatggccg tggtccagca gatggtccag ggaatccggt ccttcagcct ccttgccatc
    32581 cccttcttca tcctcgcggg ggagatcatg agccagggag ggatctcccg gaggatcgtg
    32641 ctgttcgcga acgtcctggt ggggcgggtg cggggcgggc tggcccaggt caactgcctg
    32701 gacagcatgt tcttcggggg catctccggg tccgccgttg cggacgtctc ctccatcggg
    32761 gccctcatca tccccatgat gaaggagaag ggctacgacg acgacttctc cgtggccctg
    32821 accgtgacca gcgcctgcca gggggtcatc atccccccca gccacaacat gatcatctat
    32881 tccctggccg ccggcggggt atccgtggga cggctcttcc tgggcggctt ccttccgggg
    32941 atgctcctgg gattctccct gatgatcctg agctacatct acgctctcaa gcgcaactac
    33001 cccaaggaga agaagtacag cctcaaggag gccctactca tcaccaagga cgccttcctg
    33061 ggcctgttca ccgcggtcct catcatgggc ggggtcatct cgggcgtctt cacagccacc
    33121 gagtccgcgg ccatcgcggt cgtgtacgcc ttcttcatca ccttcttcgt gtaccgggag
    33181 atacctctcc gggccatgaa gggaatcctg tacaactccc tgaagaccct ggccatggtg
    33241 atggccctga tcgcctccgc cagcgccttc ggctggatga tggcctacct gcggattccg
    33301 gcgatggcca cggaactgct cctgtccatc acccacaaca agaccgtgct cctcatcctg
    33361 gtcaacatcc tgctcctgct cctgggcatg atcatggaca tggccccgct gatcctgatc
    33421 accacgccca tcctctaccc cgtgttcgtg ggtgccctgg ggatgtcccc ggtccacttc
    33481 gggatcatgc tgatcctgaa cctggccatc ggcctgacca ccccgcccgt gg
//
LOCUS       DAOTEI010000015        33059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_884_length_33059_cov_5.457672, whole genome shotgun sequence.
ACCESSION   DAOTEI010000015 DAOTEI010000000
VERSION     DAOTEI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 33059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33059
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_33059_cov_5.457672"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="P5313_02560"
     CDS             <1..532
                     /locus_tag="P5313_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRY79278.1"
                     /translation="VYCGDARNNMGNALMIGSAKMGMHFVALAPKELWPNESLTGEMR
                     KVAAGTGGKIEFFDISKADEALKGADVLYTDVWVSMGEEAKFAERIAQLEKYRVTMDF
                     VRKTGNPDCIFLHCLPAFHDLETQVGRDVQKEHGLAEMEVSDEVFRSKHSVVFDEAEN
                     RLHTIKAVMVATIGDL"
     gene            726..1382
                     /gene="deoC"
                     /locus_tag="P5313_02565"
     CDS             726..1382
                     /gene="deoC"
                     /locus_tag="P5313_02565"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRY79279.1"
                     /translation="MIDLSCVQANHDEAEIAAMADAARTYGVFAVFALPAHTGYLKDL
                     IRDTDIKLGGTVGFPSGSVTTETKLDEVAQLKRIGVDELDMVVNITWLRSGRYDRVLD
                     DVRAVVDAAAGLPVKLILEVTYLDEERIRRGCEIGVQAGVEFVKSGTGWSGSPTTLDH
                     IRIMADAVGGRCKLKAAGGVRDRETLLAMHTLGVTRFGIGTRTAKGIFSGPQGGSDGY
                     "
     gene            1372..2907
                     /locus_tag="P5313_02570"
     CDS             1372..2907
                     /locus_tag="P5313_02570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRY79280.1"
                     /translation="MGTDKILAFDLGTGGCKASLYDLTGLCLGSDFRAYSTRYPRPGY
                     HEQKPEDWWDAVVRSSRALVGSGLCDPDSVKGIGLSGFSLGMVPIGEDGRSLLEWVPI
                     WSDARPETGDLEPFFREVPEDEWYSITGNGFPPALYTVFKILWFRRELAKEFSRARTI
                     LGSKDYINLRLTGVPATDHSYASGTGIYDLTARAYSDRLLAASGLPRSLFPEVVPSTR
                     ILGPLLPEAAAALGVGRDAVVVAGGVDNSCMALGARNLEEGSVYNSLGSSSWIAVTSR
                     RPLLDRTYRPYVFEHVVPGMYNSATAIFSAGTSHSWVLRELCRDLADPADPGAIYERT
                     EALAREVPPGSRGLLFNPSLAGGSSLDPSPRIRGAFLGLTLEHTRADLVRATLEGICL
                     GLRVALDVLASLTTVQDEMILAGGGAKSPLWRQMLSDVLGKRVLKTSVDQQAAALGAA
                     AVAAVGIGAWKDFSRIRDLHEVQARHEPDPSVSGIYAGILPAFREASRYLSGYGDSLG
                     SRP"
     gene            3230..4270
                     /locus_tag="P5313_02575"
     CDS             3230..4270
                     /locus_tag="P5313_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY79281.1"
                     /translation="MKKLTSLLLIACLAAAVLTGQTVKTNYTVGHVVYDLKHQYHQAV
                     AKEIVDYGKKLYGAKVIVLDGKGDSGEILKAVENLIAQKVKAISVHSPDQAMTLAAIK
                     MARAKGIPVVTTLVWPAEKIAPHVQPLETPSSFLMGQRAAQQWLAANPGKPCRVAILD
                     FGKFPPVEVLRTTPFWEGVKAVDPKAVLVTQLNGQGDTTKSMEITLDILQANPEVNII
                     FGANDEMALGALAACEQIGRGKMANGKPLTEVIAGLDGNVSAMIKIFDPNCSFKLTHG
                     AVKDVARAEMDTMIGMLTGKIDPNKWSETQVLSKVTDYWNSTVEEAQVFLQDNFLYKG
                     NLKDDIAKAAKK"
     gene            4400..5362
                     /locus_tag="P5313_02580"
     CDS             4400..5362
                     /locus_tag="P5313_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79282.1"
                     /translation="MGANSRPIQVDRQKIVLISILAAIIAVMSFLSPKFLQVSNFQNI
                     LLQLAPLIIIASAANLLMITGNFDLSIGSVLAFCAIMHAFLSKQGVPIGLSIVICCVL
                     AVLWGVSNGIMVGVLNVNPVIATMGTMYAARGFAFLIARWDGGANISAGLPGNFSDFG
                     RTNLFGVLPIIIVFMVIVVALFIFIERKTVLGRYSFAIGGNRRSSVLSGINVPGMMIV
                     LYSLVGLLAGFCGVVQVSRVGSGFPNIAEGMEFDVVVAIVLGGTSMLGGSGSTAGMIL
                     GAFIVGTAANGLNLLNVPYFYQTITKGLLLIVSVFTDQAIRRRG"
     gene            5377..6912
                     /locus_tag="P5313_02585"
     CDS             5377..6912
                     /locus_tag="P5313_02585"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY79283.1"
                     /translation="MNSGDAILMSIRGLSKRFPGTQALDEVSFDIRRNSVHCIIGENG
                     SGKSTMIKILTGALERSGGELSFEGRIYDPKSIREAMSEGVTALYQELNVVDDLTVGE
                     NLTLGREKSRLGMIRSGEIPPTLVENLKSLDETIKLSDRVGDLSVAQRQVIEIARAIS
                     TDCKMLIMDEPTAALSEEETGRMFKVIRKLSAKGITIVYISHKLSEVFEIGDRVTVLR
                     DGRVVGDRTVDEIRNGSGCDSESEACHELIRMMLGKVVQEEYVPSRNRTEETLLEARK
                     IRTGKLKDVSFELRRGEILGFYGLVGAGKTEIAQVLYGIDPFQGEILMDGRPVRLSGV
                     SKALGAGITLIPEERRADGIFGELSIRENIPMMRMEKVLRNGIINRTLENQLADRSIK
                     SMSLATNSREKKVSQLSGGNQQKVVISKCLNRESTVLLMDEPTRGVDVGAKQEIHDII
                     RNLANQGKGVIVFSSELPEILQLCDRIVLLYEGAVRTVLDNGRGLDGDRIMALVAGRE
                     ASA"
     gene            6909..7889
                     /locus_tag="P5313_02590"
     CDS             6909..7889
                     /locus_tag="P5313_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79284.1"
                     /translation="MKRSMDLKRAVRDPKTLMLTLLVLLNLFFALNSEAFFSLENYFN
                     MLKQSAMIIIVASAATILMMTGNFDLSTGSNLALTGVVYSLLLSSGMDLLPAALLAIL
                     CGSSVGIVNGLLVARIEFPPFIATLGTMYIARGLALILAGGSPVRGEKIPENVTILAR
                     GDWMGIPIPVYFLVLFVVLFRIVEGRSIFGKYSMTIGGNRNAAFYSGIPVGRIVFWNY
                     QFVGILASFAGILMASRLAAGDPRIGVGFEFDVILAILLGGTSLKGGKGSVLGTLIGA
                     LIVAVLGNGLDMMNILTFWQGILKGIIFVLAIVVNEKILKNVGKASTRAA"
     gene            complement(7971..9524)
                     /locus_tag="P5313_02595"
     CDS             complement(7971..9524)
                     /locus_tag="P5313_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRY79285.1"
                     /translation="MEQTYLAGIDIGTSGAKAIVFDRGGTPLASSYHEYGSTYPRPNW
                     VEQDADQIVESAMTSMREAVVRSGAAPDRIVSISLSAQRCCGIFLDENGRLIRPMISW
                     QDNRPVEEIADIARIVPEGEYYRKTGFPNSTTWLLGKMLWVRKHEPETWKRTARIVQM
                     HDYFLRALGAEEYFVDFNDAGFFGFFDSRTCDWDPELLSAFDIPRNILPIPRASGSSA
                     GTVSARGAERSGLAAGTRIAIGAGDQCAGSLGAGVVRRGTLSISMGTAGAVTALLDSP
                     YRDPNGRIMITNHSERGKWLLEGYQAAAASVFRWFKEEIAGVETAEAARLGRDPYDLI
                     GERAAGAPPGAKGLVFLPYLAAAGTPRYNPDARGTLFGLTFAHDRACLARAFMEGITL
                     DMQDMIRAIKAAGAGVDTVRLLGGPTKSVFWNQMQADIYGATVQTLKIGDATVLGAAI
                     LGGLGAGIFSSVDEGTREMVRVDRTFEPDAGRSAYYRELYEVWLSAYEGLAGSGAFGK
                     IAEIQKKHG"
     gene            9731..10612
                     /locus_tag="P5313_02600"
     CDS             9731..10612
                     /locus_tag="P5313_02600"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY79286.1"
                     /translation="MNRIELVVDAGAVCGESPIWDPVSGLLLWTDTASPFVHAWDPAS
                     RTASSRKTDRTVRSVARARDGALALICADGVYTLEKGASAARLLCDPERGRAGMMPDD
                     GTVDPAGRLVFDTYHSEVLDAPEGSIYAAEPDGTVRTLDSGLRLPNGIAFSPDGRAMY
                     VAEMFAGRILRYDYDPISGRASGRSVFAEIPADEGMPDGLIADAEGFLWNARWGGWRI
                     VRYAPDGKVDRTYPMPFATATCMGFGGTGLTDLYITSATLGLSPEDRDRSRSPGGLFV
                     LRNAGKGLEEGVFGTAP"
     gene            complement(10634..11614)
                     /locus_tag="P5313_02605"
     CDS             complement(10634..11614)
                     /locus_tag="P5313_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HRY79287.1"
                     /translation="MPVIDENVLISIAKLYYVEGLSQQEIADKISVSRPTVSNLLRRC
                     KEEGIVEIRIRETNSLPFVLQEELKSIFGLRDVRVVPSSPDESRTVEATGRAAAELLA
                     RSLRDGLKIGISWGVTTYKMISELNVAPKYQGIEVTQLVGSLGSVNPIYDSYDLVRTL
                     AVKLNGGYATIQAPAVVRSMEAKEFFLKEPGIAAAIEKARGVDTAILSVSPDDPSYSS
                     LVREGFITPEESAEISLRGAVGHSCGIHFDIQGRVLDIPLNDRIVGISSEDLSRIPEV
                     ILAACGTVKANAILGALRTGLFHVLATDEAAALKILAEAKKDGARAPGRI"
     gene            complement(11680..13059)
                     /gene="lpdA"
                     /locus_tag="P5313_02610"
     CDS             complement(11680..13059)
                     /gene="lpdA"
                     /locus_tag="P5313_02610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRY79288.1"
                     /translation="MREEYDVVVVGGGPAGYVAAIRAAREGAAVALVERDRVGGTCLN
                     RGCIPTKSYLQTADAILETREWPSRGLLLDAPPRLDMRKALSCKDAAVRRLTGGVRSL
                     LESRKVEILQGTAVLRGPGRTEIEGRGILRSRAVILAGGSASARIPVPGADSRRVLLS
                     EDVLALDEVPKSLVVIGGGVIGIEMAQILSAFGAVVTVVELEDGILPGMDKGLRDVMA
                     VALARRGVAILTGVSVASFEETASGLAVVLADGRRLEAEYALLSVGRRPDLSCLGDSG
                     VAVDRGRVLVDDAMATSLPGVFAPGDLNGRKMLAHAAFTMGEAAAEAALGRPRPVRLD
                     RVPSVVYGRPELASVGLTEAEARASRDSVRVGIFPFSANGRAVASGDTEGFVKVLADP
                     EFGEILGVHIAGTVASELINEAAVLMSMEVTAFEASGTVHAHPTRSEALMEAAADALG
                     TCVHLPPRG"
     gene            complement(13075..14403)
                     /locus_tag="P5313_02615"
     CDS             complement(13075..14403)
                     /locus_tag="P5313_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRY79289.1"
                     /translation="MAQSVIMPKSGMAMEEGIIVSWLKKVGETVSPGEAIATIETDKA
                     VLDLEAEAAGTLIAILREAGDRVPVTQIIAWIGKPGEAVPESPSAEKSQPAAAATAPS
                     PGVNAGTGSASPGGGRVAATPAARRVASEAGIDLGTVPSANGVPVAAEDVLRYVRKDA
                     TPLAARMAEAAGIDPASLAREGGGRIRKADVEAAAAGASTGERLLKERPRSGDGPRPD
                     SRVPFTSIQRITGLRLARSHAEIPSVTSFAQADVTSLLAARESLNASGGLRISLNDLT
                     VRACTKALAANPRVNAVVDGDGLILKGNIDIGIAVATETGLLVPVLRDADRLSLPEQS
                     RRSRDLAERARTRRLRPEEFEGGTFTVSNIGMYGISAFTPIINPPQVGILGVGAVEER
                     LALDPDGRPVVRKVLNLCFTFDHRALDGAESSAFLAALRDLLESPIHLVL"
     gene            complement(14388..15389)
                     /locus_tag="P5313_02620"
     CDS             complement(14388..15389)
                     /locus_tag="P5313_02620"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRY79290.1"
                     /translation="MREMTYAEAIREALSEEMRRDESVFFIGEDIGVYGGAFGVSKGM
                     LAEFGPKRVRDTPISETAIVGCGVGAAMAGLRPVVEIMFSDFVAVAMDQIVNQAAKAR
                     FQFGGQFSVPLVIRTPSGSGTGAAEQHSQSLEAWFVHTPGIKVAVPSTPADAKGLLIS
                     AIRDPNPVLFLEQKLLYRTKGEVPEGDYTVPFGQAAVRREGRDVAVITYGRMVARCLE
                     AAQAAAAEGIEAEVLDLRTLAPMDREAIIRTVKKCGRVLIVHEACRTGGVGGEIAATI
                     ADSEAFFWLDAPVRRLCGEDTPIPYNRNLEDSIVPRVPGITEEILRLARREASWPRA"
     gene            complement(15406..16362)
                     /locus_tag="P5313_02625"
     CDS             complement(15406..16362)
                     /locus_tag="P5313_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRY79291.1"
                     /translation="MGVPRETLRELYLKMAQTRRFEETAARLFTEGKVHGTAHFCIGE
                     EATGVGVCSALAPEDLIAQTHRGHNQAIGKGMDIRRMMAEFLGKETGYCRGRGGCMHI
                     ADASVGSLGANGIVGGGIPIAVGAAFAQNYRGQDAITVCFFGDGAANEGAFHEALNLA
                     SVWKLPILFVCVNNYYAMSTHLSLHMNIKDISVRAKSYGIPGFSLDGNDVVAVYTATL
                     EAKKRILENGPELMVLNTYRIMGHSKSDPQTYRTRDEVEAWKKKDPLLLFRNRLVEAG
                     EFLASDLDALDAQAARDVAEAVEYAESSPEPKVEEALQDVLA"
     gene            16739..17908
                     /locus_tag="P5313_02630"
     CDS             16739..17908
                     /locus_tag="P5313_02630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRY79292.1"
                     /translation="MYPFEFDMTTKAIFGEGAVARVGELAAGFGKKALLITYDEKFVK
                     EVGFYQKVADSCKAAGVTLLSAFGVKSNPTVEHAAGVIALAKKEKPDVLIALGGGSAM
                     DEAKFVGVAAGYDGDPWDFATGKTPITKTLPLIAVVTIPATSSELNGTSVMSYEKIQR
                     KDGFLSPLMRPKFAVLDPELTYTIPLKQTAYSAADIVSHLLEAYIGHGLDWAPFQEHY
                     CQGSIRTIMECMDRILANPRDKEARAQMMWTASYAWNGFYPCGLGPADATIHVLGHSL
                     SNFYDTPHGAAMSVTIPATMRYFLNTKTKRLAGIAREVFAVREADDLAAAKAGIAAIV
                     AWFKKIGTPTTLGEAGIPNEAIAKMAPDALKTAQAWGLGDLYSLETCVKMFELCK"
     gene            18038..19210
                     /locus_tag="P5313_02635"
     CDS             18038..19210
                     /locus_tag="P5313_02635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY79293.1"
                     /translation="MPDLVTSVSRRMLDIPREDWDALAAPAETPLLSWGFLALLEESG
                     SIRPETGWKAAHVLLRDGKTGRPAAAAPFYVRGDSWGEFVFDQGFARAAERAGYPWYP
                     KLSGVVPVTPAPAWRVLTAAGGDGLDAATQTGRLLDAAEGLAREGGLAGVHVLWPDEP
                     AAGLLRSRPGWTAWEHQAFLWTDEGYGDFRGWLDSFSKNMRRNVLRERAALAASGVRT
                     RIVGAREAAADPGILALMADLYEAHNDRFGPWAARFLTRDFFLRLPEFLPEGWILGRA
                     DRGGHTVGLSFLFEGRDRLYGRYYGASEDIPFLHFELCYYLPVEYALSRGLRAFDPGM
                     GGEHKARRGFRSYTTPSFHRPFARPVARFLEGILPELGREEAAAAAALNLDLPYKV"
     gene            complement(19227..19607)
                     /locus_tag="P5313_02640"
     CDS             complement(19227..19607)
                     /locus_tag="P5313_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY79294.1"
                     /translation="MNNGNAGSVGMLRVYPGGMDTEERRALEEGRWEWTDDPAGADLV
                     PVSRPGFRGPVTPVFGAPSLSPREREILEYLADGWSNEEIADRLGLSLATVKFHAGSL
                     YRALGVGRRTEAVREARLLGLLDW"
     gene            complement(19608..20492)
                     /locus_tag="P5313_02645"
     CDS             complement(19608..20492)
                     /locus_tag="P5313_02645"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HRY79295.1"
                     /translation="MGEFAKEFEGTLREVVESWKGSLVHIGGPGIPYRTGIVVKEGLA
                     ASLARSARDGEEVPVVTASGPATGRVEAWDGESGLVLIRIGDGAAPVPAGAGTEGLDA
                     PRVGTPVLRLAYPSFQGIEAAFSVVRFSGGSTEWGRRKASAHFQVDGPAYPGFLGAPV
                     VDLAGTLRGVVISNEDGNDGWVLPAGPWRVAVDELAKEGTRIRGWLGISIGTVRLSKA
                     QSSSAGRETALMVRETAPGGPAEAAGVQPGDLILGADGAGLGDDGIPPLRVGRPVILD
                     ILRGSERLALQVLPSPRS"
     gene            20615..21547
                     /locus_tag="P5313_02650"
     CDS             20615..21547
                     /locus_tag="P5313_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY79296.1"
                     /translation="MDRDEDLLDSYSKTVSGVAESARDSVAHVRVRRGRDPEAGGSAF
                     AFTPDGFLLTNSHVLRDAEAVEAVLPGGRRCEAAIVGDDPATDLAVLRIYGESLPPLE
                     FGDSSRIRVGQIAVAIGNPLGFESTLTAGVVSALGRSLRSVTGRLIDSVIQTDAALNP
                     GNSGGPLLDSRGRVIGVNTAIIAQAQGLCFAIPSDTAKAVAAALIRDGRILRGWLGIG
                     GQGVPIPRKLAHHYGLEGPEGAFLSAVEPGSPADRAGLKAGDILVRFGRERIQGLDDL
                     LRLLGPDSPGREFPVEALRGPDRLLAWVIPVPAP"
     gene            complement(21593..22858)
                     /locus_tag="P5313_02655"
     CDS             complement(21593..22858)
                     /locus_tag="P5313_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2088 domain-containing protein"
                     /protein_id="HRY79297.1"
                     /translation="MIGYANLDGLSLTKVVRDFPGAGIPDIEAAVAEEFSRPGAPFRG
                     IRPGARIAVAVGSRGIANLARIVRAVVRELSARGALPFVIPAMGSHGGATAEGQREVL
                     AGYGITEAGVGAPVVSSLDIVELPAAPDGTRVYMSRDAWGSDGVLLVNRVKPHTDFHG
                     IHESGLVKMAVIGLGKHAQALEIHSHGVAGLRERILPAARVVLGTGKVLGGLGIVENA
                     REEPEILRLVSGEGLPEADRELLERARELQPRLPVDDLDVLLVDEMGKDVSGVGLDSN
                     VIGRMRVRDQGEPDRPRISSVAVFDLTEASHGNALGVGLADVITRRLRDRIDLGATFE
                     NVVTSGFLERGKIPLSAESDRAVVEIALRGASVRDLFRARILRIRNTLRLGEVWASDA
                     LLPELAGREGVEITGERATLLDGHRLGKF"
     gene            complement(22869..24800)
                     /locus_tag="P5313_02660"
     CDS             complement(22869..24800)
                     /locus_tag="P5313_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79298.1"
                     /translation="MKTSIRTQLGALLLFWTASAGSLYLASWIQSDSGTVEVSPVRIE
                     APAGTVTAKLYRPASARPGSPVPGVVLIHGYQNDKETSSAFALELARRGIAALSIDAF
                     GHGSAEPGMRARGFTPYKYPNFERRISGPERYKVMMNFSALDFFLPKYSEGLADTSMG
                     GKAAYAWLRSRDFVDPAALGVTGHSMGTWASWSVAADFPDHRAVVLQCGEIIPEGFYD
                     PSAVRFNNVLLLQAVWDEFAFFRDYRNTVKGLEATDLRYRVFMGQEGPVEWDRTYGSF
                     ADGSARRMELLYTNHRLVTHNGKGIAAAVDWFARSFDLEPALPASDRTYLRKELLVGL
                     AMLSALASLLPLLSILLRIPLFAGAAQPLPSRVLLEGRAWRRAAAGTVLLGAATFPFL
                     TQLGHGLAPLPESVFRMTVGNGFLVWLTFLMVVSLVLLVRWFRKGPGRAAGATLHDLA
                     LAPAERPAAVPWPVVGKSVLLAFLLAGSLYAQTALYDALFGLELRFIWPFFRVFTPER
                     FGQFWVYLPFYGAFFFVNGGVKLFGQLRLPERASPAATQAAWWLTACGVMLGSLLVWA
                     FIEYVPFFLGIGPGADLLLTPLFGGPFMSILLLTIPQFALMFFLAAAMFRRTGFVYVG
                     SLTVGILAAWMLSGGSAIF"
     gene            24957..25922
                     /locus_tag="P5313_02665"
     CDS             24957..25922
                     /locus_tag="P5313_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79299.1"
                     /translation="MNYLFHYYAVRWLAREAGVPEPEAETIARSSQFVDEAVRPLRVE
                     TGGVPYDLRVTQDYLFWDESILSEVYLPFHFLPGDPEEAGRKRRDGARNPGAVTPNGG
                     VARELLVEALKTGDPYRIGIALHSFADGWAHQNFTARWEDFNALDGSGALPPVGHLQA
                     LTNPDNPSRLWADPRLLPELYRIDNAARFLEAARKIFRYLRTWLRKPFGDEDLLLPKL
                     EGLLRSPGDREGVEAAFIVDGDLPKYEKGAWAREAGVLDGWDRGEGSRGYDKLLWLKS
                     QAERRLGSGVRTVRAGPGFFESDLYRWHEAAGEHRRAAKAALANF"
     gene            complement(25961..26704)
                     /locus_tag="P5313_02670"
     CDS             complement(25961..26704)
                     /locus_tag="P5313_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRY79300.1"
                     /translation="MNFPCFRRGPGAARPPGPARAAVAAAACAVLGSCAVLSPVRLES
                     GVVGPTLLVVAGIHGDEPSGVRAARALADRGPALRGRLLVLPEADPAALEAETRTAPG
                     AGDLNRAFPGRRLGAPAERLAFELFRLIRNARPDLVLDLHESDGTWTEGPGPFLVVPA
                     LPRAAELALAMLDRPGMEEFSYTGGPPGGSLAAETSRLLDVPALIVEIPDTVPAARRM
                     ELHRAAVEAAMAVLGMLDTGEEGRIKPCR"
     gene            complement(26701..27831)
                     /locus_tag="P5313_02675"
     CDS             complement(26701..27831)
                     /locus_tag="P5313_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HRY79301.1"
                     /translation="MKRHHATALAVTLLALAAAAAASIRFLDHRKPEPIVRGPGVTEV
                     RMLSEWLPDLAGSPGDTEVYVFRGASPGSSMLVLGGTHPNEPSSFLSAVLLIENALVS
                     AGTLYVIPRANASGFTHNDPQEGAPERYSVALPGGSVRTFRYGSRATNPVHQWPDPDV
                     YVHAASGQTLSGNETRNLNRGYPGRADGTLTERVCLGIANLIRAEKIDISVDLHEASP
                     EYPVINAIVAHERAMPLASQVVLGLEFLDIKIGLEPSPKNLRGLTHRELGDATDTLAV
                     LMETANPSQGRLRGRTDEALVLKGRDPYYVKAQALGRLYVPFDENGHPIEERVGRHLS
                     GISEFAFALSSERPDRPLEFAGVPEYGALLEKGLGAFLKPLP"
     gene            complement(27841..27984)
                     /locus_tag="P5313_02680"
     CDS             complement(27841..27984)
                     /locus_tag="P5313_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79302.1"
                     /translation="MTYLYLAIAASVLGLTAWNLWTEKDWRKQATAAMVAIPLLLRVL
                     LIK"
     gene            complement(28001..29269)
                     /locus_tag="P5313_02685"
     CDS             complement(28001..29269)
                     /locus_tag="P5313_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY79303.1"
                     /translation="MPFELVIFLAMVGVFAAGCFAAKLPVSLSMLLAACAGLLVSGNG
                     LPVRHLVEGTFGYVDTILVIATAMVFMRTVQESGALEALNAVILRRFRTVPALLLPLL
                     MLVAMFPGMITGSSSASVLTAGAVVAPVLILLGLPAPVTGAFIAMAGILGMIAPPVNI
                     PAMIIGGGIDMPYVGFEVPLALLTFPLAVLFSLWFGLPHVKVVEWEVLAAKLDLETYK
                     KYGIRLFLPILVVAVLMVLGKTLPQVFGMGLPVVFLIGAALGLVVGKRFNILSASKAA
                     VNDALPVLGILAGVGMFIQVMTLTGVRGFVVVSALSVPPALLYAAIAVSIPLFGAVSA
                     FGASSVLGVPFLLALLGRDQIVTAAALSLIASLGDFMPPTALSAIFAAQVVGESKYSR
                     VLVKLVVPSLVIIGWALAFIVFSKQIRALY"
     gene            complement(29279..29830)
                     /locus_tag="P5313_02690"
     CDS             complement(29279..29830)
                     /locus_tag="P5313_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HRY79304.1"
                     /translation="MRRAILSVFVLLAAAAALSAQAPAAFAEKPALLTSVGQSADIEM
                     VKVLLNRGKVAHTADPLVTAAKLPGGAKTLVLAVGGSSKGLGAAGISTEAEIDRAKAL
                     IKRARELGMKIIAIHVGGEARRGTLSDAFISLCVPVSDYAIVVEDADKDGLFTKLASQ
                     AKIPLVKVDKITAAGVPLAAAFK"
     gene            complement(29948..31636)
                     /gene="ggt"
                     /locus_tag="P5313_02695"
     CDS             complement(29948..31636)
                     /gene="ggt"
                     /locus_tag="P5313_02695"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY79305.1"
                     /translation="MKKLFSVFVLVAFAAALIAAQTPVNLYGRAAQGAKGVVAAAKPE
                     ASQVGIDILKKGGNAVDAAIATAFALGVLEPNASGIGGGGFMVIKMANMKEAVVIDFR
                     ETAPAAAKPDMFKFGEDGKVIDNASVVGGLSVGVPGEVAGLLYALENYGSKKMNREKV
                     LAPAVEWAEKGIPVTVNLASIIQSELGKINKYPATAKIYTKDGLPYELGETIKNPDLA
                     ATLRAIAKDGKDAIYKGEMAERIAKAARDAGGILTAADLANYQVKIRKPVTGTYRGYT
                     IVSVPPASSGGTHVIQILNILENMDIAKMGPGTPATTHAWIEALRLAFADRGKYMADA
                     DFVKVPLKGLANKDYAKVLAAKIDMAKAMGSATADDPTKFESGSTTHFSVMDKDGNMV
                     AVTKSINYFFGSGVTVPGTGFILNDHMDDFALKPGHVQSVEPGKRPLSSMTPTLVLDP
                     DGQPFMTVGSPGATRIIAAVAQVISNVIDHRMSVQDSIMAPRVFAMASGQVQLEGRAS
                     INTLKGLEALGHNVNVRADWDAYFGGVHAVLFDRKAKVLYGGADPRRDGQAAAF"
     gene            31779..32723
                     /locus_tag="P5313_02700"
     CDS             31779..32723
                     /locus_tag="P5313_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79306.1"
                     /translation="MRPTRTRPTRRNGAPALVLTATLVLASCATRIPVAQEADPSAGL
                     PREASVYLRADRPLLEEAAGALLAPADLRAVRPFLARTDRVTAALVLPRNDSGGSGAG
                     PGAPETSEVFGIAEGTYPAGAAAFRLRLSREWRKEGRTLVRRDGKLRIAFAGRQVLAA
                     STSDLEPLLDRLAVPGPHPVPGDLRPYWEAPGALWIPRPGETAERILGAGAPRVPARG
                     MLLSLNPGEAVTGYSGTWIFAFESERDARVYAPICRLAHLAFIRVVHGTGTEAAAALE
                     RTVWTAEGTLIKASGFPLDLSSLFRALKAALPESSQGS"
     gene            complement(32766..>33059)
                     /locus_tag="P5313_02705"
     CDS             complement(32766..>33059)
                     /locus_tag="P5313_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79307.1"
                     /translation="RYDLGTELARLSAAGLPILLMQGDLDLQVTMEEFDRLRLSVPDA
                     EAVLLPDTNHILKLVGNEVEENYESFSDPSYPLSPGVVPALADFLERVPARPE"
BASE COUNT         5593 a        11163 c        10944 g         5359 t
ORIGIN      
        1 ggtctactgc ggggacgccc ggaacaacat gggcaacgcc ctcatgatcg gctccgccaa
       61 gatgggcatg cacttcgtgg ccctggcccc caaggagctc tggcccaacg agagcctgac
      121 cggggaaatg cggaaggtgg ccgccgggac cggcgggaag atcgagttct tcgacatttc
      181 caaggccgac gaggccctca agggcgccga cgtcctctac acggacgtgt gggtgtccat
      241 gggcgaggag gccaagttcg ccgagcggat cgcccagctg gagaagtacc gggtgaccat
      301 ggacttcgtc cgcaagaccg gcaacccgga ctgtatcttc ctgcactgcc tgcccgcctt
      361 ccacgacctg gagacccagg tgggccggga cgtccagaag gaacacggcc ttgcggagat
      421 ggaagtctcc gacgaggtct tccgcagcaa gcactccgtg gtcttcgacg aggctgaaaa
      481 tcgactgcat acgattaagg cggtgatggt cgccacgatc ggagatctgt agaccgcctc
      541 gattccgcgg cggcgcctcc cggcgcctcc gcggcccttc attctttcct gccccatcgg
      601 ccaacccttc gataccccat gaattgactg gacacctatc ccaagcgggt ttactgtgtc
      661 cgcactgcgg catagaccgc atcgaaaggc gggcgaacgg gatggacaac cggactatgg
      721 cttcgatgat cgacctgagc tgcgtgcagg cgaaccacga cgaggccgag atcgccgcca
      781 tggcggatgc cgcccggacg tacggagtct tcgccgtctt cgccctgccc gcccacacgg
      841 ggtacctgaa ggacctgatc cgggatacgg acatcaagct cggcggcacc gtcggttttc
      901 catccggatc ggtcaccacg gaaacgaagc tcgacgaggt cgcccagctc aagcggatcg
      961 gcgtcgacga attggacatg gtcgtgaaca tcacctggct ccgctcgggc cggtacgacc
     1021 gggtgctcga cgacgtacgg gcggtcgtgg acgccgcggc cggcctgccg gtcaagctga
     1081 tcctggaggt cacctacctc gacgaggagc ggatccgccg gggctgcgag atcggcgtcc
     1141 aggcgggggt ggaattcgtg aaaagcggga ccgggtggtc cggatccccg acaaccctgg
     1201 accatatccg gatcatggcc gacgcggtcg gcggacggtg caagctcaag gccgccggcg
     1261 gggtccggga ccgggagacg ctcctggcca tgcacaccct gggcgtcacg cgcttcggga
     1321 tcggaacccg gaccgcaaag ggcatctttt cgggaccgca gggaggttcc gatgggtact
     1381 gacaagatcc tggccttcga cctgggtacc ggcggctgca aggcctccct gtatgacctc
     1441 accggtctct gcctgggcag cgacttccgc gcctacagta cccggtatcc ccgcccaggg
     1501 taccacgaac agaagcccga ggattggtgg gacgcggtgg tccgaagctc ccgggccctc
     1561 gtgggatccg gcctttgcga tccggattcg gtaaagggca tcggcctttc gggtttcagc
     1621 ctgggcatgg tgccgatcgg agaggatggc cgatcgctcc tggaatgggt accgatctgg
     1681 tcggatgccc ggccggaaac cggggacctg gaacccttct ttcgcgaggt cccggaggat
     1741 gagtggtatt cgatcaccgg gaacggcttc cccccggccc tctacacggt cttcaagatc
     1801 ctctggttcc gcagggaact ggccaaggag ttttcccggg cgcggacgat cctggggtcc
     1861 aaggattaca tcaacctgag actcacgggc gtcccggcca cggaccattc ctacgcctcc
     1921 ggcacgggaa tctacgacct cacggctcgg gcgtattccg accgcctcct tgcggcctcc
     1981 ggcctgcccc gctccctctt ccccgaggtg gtcccctcca cccggatcct gggacccctc
     2041 cttcccgagg cggccgccgc cctgggggtg ggccgggacg cggtggtcgt cgccgggggg
     2101 gtggacaact cctgcatggc cctgggagcc cggaacctgg aggaaggctc ggtgtacaac
     2161 tccctgggct cctccagctg gatcgcggtc acctcccgcc ggcccctcct ggaccgtacc
     2221 taccggccct acgtgttcga gcacgtcgtg ccggggatgt acaactccgc cacggcgatc
     2281 ttctccgccg gcaccagcca ttcctgggtc cttcgggagc tctgccggga cctcgcggat
     2341 cccgccgacc ccggagcgat ctacgagcgt acggaagccc tggcacggga agtccccccg
     2401 ggttcccggg gcctgctgtt caatccctcc ctggcgggag gcagcagcct ggatcccagc
     2461 ccgcggatcc gcggcgcctt cctggggctc accctggagc acacccgggc ggacttggtc
     2521 cgtgccaccc tggaaggcat ctgcctgggg ttgagggtgg ccctggacgt cctggcctcc
     2581 ctgacgacgg tccaggacga gatgatcctg gcgggaggcg gcgccaagag ccccctctgg
     2641 cgccagatgc tctcggacgt cctgggcaaa cgtgtgctca agacctccgt ggaccagcag
     2701 gccgcggccc tgggcgcggc ggcggtggcg gcggtcggaa tcggggcctg gaaggatttt
     2761 tcccgaatcc gggacctcca cgaggtccag gcccgccacg agccggaccc ctcggtatcc
     2821 ggaatctacg ccggcatcct gccggctttc cgtgaagcat ccaggtacct gagcggatac
     2881 ggcgactccc tcggatcccg gccctagttc cgatccagga tccatcctca tcccgatgcc
     2941 ggcacgcgcg gcccgcggcc ggcgttcccg cgagtccgga cttgccgccc cgatacaggt
     3001 ttcgattgac aactgtcaat tcatgcccta caattccaac agatgtaatt gttgtagcaa
     3061 tgtatcaaga ttcgtgatga ttggattaca tatgtaaccc ggatcgtcat ggattcccaa
     3121 cggacgaatt ccgggacccc gagcgcccga ttcgataccg ggggaaagtg tggcgggtga
     3181 acattccggc gggagagccg gtaaacaagt ggaaacggga ggagttctga tgaagaagct
     3241 cacaagtcta ttgctgatcg cgtgtcttgc ggcggcggtg ttgaccggcc agaccgtaaa
     3301 gacgaactac acggtgggac atgtggtcta cgatctcaag catcagtacc accaggcggt
     3361 ggccaaggaa atcgtcgatt acgggaagaa gctgtacggc gccaaggtga tcgtcctgga
     3421 cggcaagggc gattccgggg agatcctcaa ggccgtggaa aacctgatcg cccagaaggt
     3481 gaaggccatc tccgtgcaca gccccgacca ggccatgacc ctggcggcga tcaagatggc
     3541 ccgggccaag ggcatccccg tagtgaccac cctggtctgg ccggccgaga agatcgcgcc
     3601 ccacgtccag cccctcgaga cccccagctc cttcctgatg ggacagcgcg ccgcccagca
     3661 gtggctggcc gccaaccccg ggaaaccctg ccgcgtcgcc atcctcgact tcggtaagtt
     3721 cccccccgtc gaggtccttc gcaccacccc cttctgggaa ggcgtaaagg ccgtcgaccc
     3781 gaaggccgtt ctcgtgaccc agctcaacgg ccagggcgac accaccaagt ccatggagat
     3841 cacccttgat atccttcagg ccaatcccga ggtgaacatc atcttcgggg ccaacgacga
     3901 gatggccctg ggagccctgg ccgcctgcga gcagatcggc cgcggcaaga tggccaacgg
     3961 aaagcccttg accgaggtca tcgcgggcct ggacggaaac gtctcggcca tgatcaagat
     4021 cttcgacccc aactgctcct tcaagctgac ccacggagcc gtcaaggacg tggcccgggc
     4081 cgagatggac acgatgatcg gcatgctcac cgggaagatc gatcccaaca agtggtccga
     4141 aacccaggtt ctgagcaagg tgaccgacta ctggaacagc accgtcgagg aagcccaggt
     4201 cttcctgcag gacaacttcc tgtacaaggg caacctgaag gacgacatcg ccaaggccgc
     4261 gaagaaataa tccggagcac tccttcggag gcgagggacg gcacgccgtt tctcgccttt
     4321 ttttagggtc tggccgcccc tccgtccccc gggacgggcg ggcggcctcg gattaacgca
     4381 atccctaagg atattgtaga tgggcgcaaa ctccagaccg atccaagtcg accggcagaa
     4441 gatcgtgctg atttcgatcc tggcagcgat catcgcggtg atgagcttcc tgtcccccaa
     4501 attccttcaa gtcagcaact tccagaacat cctgctccaa ctggcgccgt tgatcatcat
     4561 tgcctccgcc gcaaacctgc tcatgatcac cggcaatttc gacctctcga tcgggagtgt
     4621 cctggcattc tgcgccatca tgcacgcctt cctttccaaa cagggtgtac ccatcgggct
     4681 gtcgatcgtg atctgctgcg tgttggccgt cctctggggc gtatccaacg ggatcatggt
     4741 gggcgtcctc aacgtcaatc ccgtgatcgc caccatggga acgatgtacg ccgcccgggg
     4801 cttcgccttc ctgatcgccc gctgggacgg tggcgccaac atatccgcgg gcttgcccgg
     4861 caacttcagc gacttcggcc gcacgaacct cttcggcgta ttgcccatca tcatcgtttt
     4921 catggtgatc gtggtcgccc tgttcatctt catcgagagg aagaccgtac tgggccggta
     4981 cagcttcgcc ataggcggca atcgaaggtc ctcggtactc tcggggatca acgtacccgg
     5041 gatgatgatc gtcctctatt ccctcgtggg tctcctggcg gggttttgcg gggtggtcca
     5101 ggtctcccgt gtcggatccg gctttcccaa catcgcggag ggcatggagt tcgacgtcgt
     5161 ggtggccatc gtcctgggcg gaaccagcat gctgggcggc tcgggctcca ccgcgggcat
     5221 gatcctgggg gccttcatcg tcggtaccgc ggccaacggg ctcaacctgc tcaacgtgcc
     5281 ctatttctac cagacgatca ccaaaggcct gctgctgatc gtctcggtct tcaccgatca
     5341 ggcgatccgc cggcgcggat gaacggggag gcggaaatga acagcggtga cgcgatcctg
     5401 atgtccatcc gaggactgag caagagattc ccgggaaccc aggccctgga cgaagtatcc
     5461 ttcgacatcc gcaggaactc ggtccattgc atcatcggtg aaaacgggtc cggcaaatcc
     5521 acgatgatca agatcctgac cggagctctg gaacgaagcg ggggggaact ctccttcgag
     5581 ggccggatct acgatcctaa aagtattcgc gaagccatgt cggagggggt gaccgccctc
     5641 taccaggagc tgaacgtagt ggacgacctc acggtcggtg agaaccttac cctgggtcgg
     5701 gagaagtccc gcctcggcat gatccgaagt ggggagatcc ctcccaccct tgtcgaaaac
     5761 ctcaagagcc tggacgaaac gatcaaactc tccgaccggg tcggagacct gagcgtcgcg
     5821 caaaggcagg tcatcgagat cgcccgcgcc atttccacgg actgcaagat gctgatcatg
     5881 gacgaaccga cggccgccct gagcgaggag gagaccgggc ggatgttcaa ggtgatccgc
     5941 aaactctcgg ccaaggggat cacgatcgtc tacatctccc acaagctgag cgaggtgttc
     6001 gagatcggcg accgcgttac cgtgctccgg gacggaaggg tcgtcggcga ccggaccgtg
     6061 gatgagatcc ggaacggatc gggctgcgat tccgaatcgg aagcctgcca cgaactgatc
     6121 cgcatgatgt tgggcaaggt ggtccaggaa gagtacgtac ccagccggaa ccggacggag
     6181 gaaacgctcc tggaagctcg caagatccgt accggtaaac tcaaggatgt gagcttcgaa
     6241 ctgaggaggg gggagatcct gggcttctac ggtctggtcg gggccgggaa gacggaaatc
     6301 gcccaggtgc tctacggaat cgatcccttc cagggcgaga tactcatgga cggccgcccg
     6361 gtccgtcttt ccggcgtgtc caaggccctg ggcgccggga tcaccttgat tcccgaggaa
     6421 cggagggcgg acgggatctt cggggagctt tccatccggg agaacatccc catgatgcgg
     6481 atggagaagg ttctccggaa cgggatcata aacagaaccc tggagaacca actcgccgac
     6541 aggtccatca agagcatgtc cctggcgaca aacagccggg agaagaaagt atcccaattg
     6601 agtggcggaa atcagcagaa ggtggtcatc tcgaaatgct tgaaccggga aagcacagtc
     6661 ctccttatgg acgaacccac ccggggagtc gacgtcgggg ccaagcagga gatccacgac
     6721 atcatccgga acctggccaa ccagggcaag ggagtgatcg ttttctcctc ggaactgccg
     6781 gagatcctgc agctctgcga ccggatcgtc ctgctctacg agggggccgt ccggaccgtg
     6841 ctggacaacg gacgcggatt ggatggagac aggatcatgg ccctggtggc cggccgggag
     6901 gcgagcgcat gaagcggagc atggacttga agagggcggt acgggatccc aagaccctga
     6961 tgctgaccct gctggtgctg ctgaacctgt tcttcgccct gaactcggag gcctttttct
     7021 ccctggaaaa ctatttcaac atgctcaagc agtcggccat gatcatcatc gtcgcctcgg
     7081 cggccacgat cctgatgatg accggcaact tcgacctctc caccggcagc aacttggccc
     7141 taacgggcgt agtctattcc ctgctccttt cctcgggcat ggacctcctt ccggccgccc
     7201 tcctggcgat cctctgcgga agttcggtag ggatcgtcaa cgggctcttg gtggcccgga
     7261 tcgagttccc gcccttcatc gccaccctgg gcactatgta tatagcccgt ggactggctc
     7321 tgatcctggc gggaggatcc cccgtccgtg gcgagaagat ccccgagaac gtcacgatcc
     7381 tggcccgagg ggactggatg gggatcccca tccccgtgta tttcctcgtc ctcttcgtgg
     7441 tcctcttccg catcgtcgaa gggagatcca tcttcggcaa gtactccatg accatcggcg
     7501 gcaaccggaa cgcggccttc tattccggaa ttccggtggg gcggatcgtc ttctggaatt
     7561 accagttcgt gggtatcctg gcgtcctttg ccgggatcct gatggcctcc cgtctggccg
     7621 cgggggaccc ccgaatcggg gtgggattcg aattcgacgt gatcctggcc atccttctgg
     7681 gcggaaccag cctcaagggc ggcaagggtt cggtcctggg caccctgatc ggggccctga
     7741 tcgtcgcggt cctgggaaac ggtctggaca tgatgaacat cctgaccttc tggcagggca
     7801 tactgaaggg gatcatcttc gtcctggcca tcgtggtcaa cgagaagatc ctcaagaatg
     7861 tcgggaaagc atctacgcgc gccgcctgaa ccggatcgaa gagatagccc ccggtgaggc
     7921 acggcctccc cgggggcttt ttactacccg gaatacaacg ggcgggccct ctagccgtgc
     7981 ttcttctgga tctccgcgat ctttccgaag gctcccgatc cggccagccc ctcgtaggcg
     8041 gaaagccata cttcgtacag ctcccgatag tacgcggacc ttccggcgtc gggctcgaag
     8101 gtgcgatcca ccctcaccat ctcccgggtt ccttcatcca cggaggagaa tatccccgcc
     8161 cccagacctc ccaggatcgc ggcccccagg acggtggcgt ccccgatctt cagggtctgc
     8221 acggtggcgc cgtagatatc cgcctgcatc tggttccaga ataccgactt ggtgggtcca
     8281 cccaggagcc gcaccgtgtc cacccccgct cccgcggcct tgatcgcccg gatcatgtct
     8341 tgcatgtcca gggtgatgcc ttccatgaaa gcccgggcca ggcaggcgcg gtcgtgggcg
     8401 aaggtcaggc cgaacagggt tccccgggcg tccggattgt agcggggggt ccccgcggcg
     8461 gcgagatatg gaaggaaaac aaggcccttg gcacccgggg gagccccggc ggcccgttcc
     8521 ccgatgaggt cgtaggggtc tcggcccaac cgggcggcct cggcggtctc aactcccgcg
     8581 atctcctcct tgaaccagcg gaagacgctg gccgccgcgg cctggtagcc ctccagcagc
     8641 catttccccc gttccgagtg gttggtgatc atgatccgcc cgttgggatc ccggtagggg
     8701 ctgtccagca gggccgtgac cgccccggcg gtccccatgg agatggacag ggtaccccgg
     8761 cggacaaccc cggctcccag ggagcccgcg cactggtccc cggccccgat cgcgatccgg
     8821 gtgcccgcgg ccaggcccga acgctccgcc ccccgggcgg aaaccgtgcc cgcggaactt
     8881 cccgatgccc ggggaatcgg gaggatgttc cgggggatat cgaaggccga tagaagttca
     8941 ggatcccagt cgcaggtccg ggaatcgaag aatccgaaga aacctgcgtc gttaaaatcg
     9001 acgaaatact cctccgcacc cagggcccgc aggaagtagt cgtgcatctg cacgatccgg
     9061 gccgtgcgct tccacgtctc gggttcgtgc ttccgaaccc agagcatctt gcctaacagc
     9121 caggtcgtgc tgttcgggaa gccggtcttc cggtagtact ccccttcggg aacgatccgg
     9181 gctatatcgg cgatctcctc caccggccgg ttgtcctgcc aggatatcat cgggcggatc
     9241 agccggccgt tttcgtccag gaagatcccg cagcaccgct gggcggagag cgagatggag
     9301 actatgcggt ccggggcggc cccggaacgg acgacggctt cccgcatcga cgtcatggcg
     9361 ctctccacga tctggtcggc gtcctgctcc acccagttgg gccgcggata ggtggaacca
     9421 tattcatggt aggacgaggc gaggggtgtg ccgccacgat cgaacacgat ggctttggct
     9481 ccgctcgttc cgatatcgat gcccgccagg taggtttgct ccatgggatg cctccggatc
     9541 cgtactagtg gttgaactta gtttatcatg ggtcaaataa aaagaaaaca gataaatatt
     9601 gtcgttcatt cgtacatatg taatttcatc tttatacata ttacatttgt attgacagcc
     9661 ggaacgtcca tggtatactc cttccaccga tccctacaat caagccgagg agtccaggat
     9721 gggaacgtca atgaacagga ttgagctggt cgtggatgcc ggagcggtct gcggggagtc
     9781 cccgatctgg gatcccgtct ccgggctcct gctttggacg gatacggcct ctcccttcgt
     9841 ccatgcctgg gatccggcct cccggacagc ctcctcccgg aagacggacc ggaccgtccg
     9901 atccgtagcc cgggcgcggg acggcgccct cgccctcatt tgcgccgacg gagtttatac
     9961 cctggagaaa ggcgcatccg ccgcgcggct cctctgcgac ccggaacgag gcagggccgg
    10021 aatgatgccc gacgacggca ccgtcgatcc cgcgggcagg ctggtcttcg acacctacca
    10081 ctcggaggtc ctggacgctc ccgaaggctc gatctacgcc gcggaaccgg acgggaccgt
    10141 ccggaccctg gactcgggat tgaggcttcc caacggcatc gccttctccc ccgacggccg
    10201 tgcgatgtac gtggcggaga tgttcgccgg gaggatcctg cgctacgact acgacccgat
    10261 ctcgggcagg gcatcggggc gctcggtctt cgcggaaatt cccgccgacg aaggcatgcc
    10321 cgacggcctc atcgcggatg ccgagggctt cctctggaac gcccggtggg ggggatggag
    10381 gatcgtccgg tacgcccccg acgggaaggt cgaccgcacc taccccatgc ccttcgccac
    10441 ggctacttgc atgggcttcg ggggtaccgg cctcacggat ctgtacatca cctccgccac
    10501 cctgggactc tccccggagg acagggaccg gagccggtcc ccggggggcc tctttgtcct
    10561 gcggaacgcc ggaaagggtc tggaagaagg cgtcttcggg acggcgccct gagttccgga
    10621 cccgagacgg gtcctagatt cggcccgggg cccgggcccc gtccttcttg gcctccgcca
    10681 ggatcttcag ggccgcggcc tcgtcggtgg ccaggacgtg gaaaagcccg gtgcgaaggg
    10741 cgcccaggat ggcgttggcc ttcacggtac cgcaggccgc caggatcacc tcgggtatcc
    10801 gggagagatc ctccgaggag atcccgacga tccggtcgtt cagggggatg tccaggaccc
    10861 gcccctggat gtcgaaatgg atgccgcagg agtggcccac ggccccccgc aggctgatct
    10921 ccgcggattc ctccggtgtg atgaagccct cccggaccag ggagctgtaa ctcgggtcgt
    10981 cggggcttac ggaaaggatc gcggtatcaa ccccccgggc tttttcgatg gccgcggcga
    11041 ttccgggttc cttgaggaag aactccttgg cctccatgga gcgcaccaca gcgggggcct
    11101 ggatggtggc atagccgccg ttgagcttga cggccagcgt acgcacgagg tcgtaactgt
    11161 cataaattgg atttacggat cccagggatc cgaccaattg ggtaacctca attccttggt
    11221 acttcggcgc tacgttgagt tccgagatca tcttgtaagt cgtcactccc caggagatgc
    11281 cgatcttaag cccatcccgg agggatcgcg ccaggagctc cgctgccgcc ctccccgtcg
    11341 cctccaccgt cctggattcg tcggggctgg agggcacgac ccggacatcc cggagcccga
    11401 agatggactt cagttcctcc tggagcacga agggcaggga attggtttcc cggatccgga
    11461 tttccacgat cccctcctcc ttgcagcgcc ggaggagatt ggagaccgtc ggacgggaga
    11521 cggagatctt gtcggctatc tcctgctggg acaaaccttc cacgtagtac agcttggcta
    11581 tggatatgag tacgttctcg tcgatgaccg gcatacatac tccgtcgtga cattcatcaa
    11641 ggtttcggga ttcatagtac aactgacagc cgatatcagc tagcccctgg gcgggagatg
    11701 tacgcatgtt cccagggcat ccgcggccgc ctccatcaac gcctccgacc gggtcgggtg
    11761 ggcgtggacg gttcccgacg cctcgaaggc cgtcacttcc atgctcatga ggacggcggc
    11821 ctcgttgatg agctcggagg ccacggttcc tgcgatgtgg acccccagga tctccccgaa
    11881 ctccggatcc gccaggacct tcacgaatcc ttccgtatcc ccggaggcta cggcgcggcc
    11941 gttcgcggag aaggggaaaa tgccgacgcg cacggaatcc cgggaagccc gggcttccgc
    12001 ctccgtgaga cccaccgagg ccagctcggg tctcccgtag acgaccgagg ggacccggtc
    12061 cagacggacc gggcggggtc ggccgagggc cgcctccgcc gcggcttccc ccattgtgaa
    12121 ggccgcgtgg gccagcatct tccttccgtt caggtccccg ggggcgaaaa ctccgggcag
    12181 gctggtcgcc atggcatcgt caaccaagac tcggccccgg tccaccgcga ccccggaatc
    12241 gcccaggcag gagaggtccg ggcgccttcc gacggacagg agggcgtact cagcctccag
    12301 ccggcgcccg tcggccagga ccaccgcgag gccggacgcg gtctcctcga aggacgccac
    12361 ggacacgccg gtgaggatgg ctacgcccct gcgagccagg gcgaccgcca tgacatcccg
    12421 gagccccttg tccatcccgg gcaggatgcc gtcctccagc tccaccacgg tcaccaccgc
    12481 tccgaaagcc gaaaggatct gggccatctc gatcccgatc accccgcccc cgatcacgac
    12541 gaggctcttt ggcacctcgt ccagggccag cacatcctcc gagagaagga ctctccggga
    12601 gtcagcgccc ggaacgggaa tacgggccga tgcggagccg ccggcgagga taaccgcccg
    12661 ggaccgcagg atgccccggc cctcgatttc cgtccttccg ggtccccgaa ggacggccgt
    12721 tccctggagg atttccacct tccgggactc caggagggac cggacgcccc cggtgagcct
    12781 ccgcactgcg gcatccttgc aggacagggc cttcctcatg tccaagcggg gcggagcgtc
    12841 caggaggagc ccccgggaag gccattcccg ggtctccagg atggcgtccg cggtctggag
    12901 gtaggacttc gtgggaatgc agccacggtt caggcaggtt ccccccaccc ggtcccgttc
    12961 caccagggct acggcggctc cctcccgggc ggctcgtatg gcggccacgt agcccgcggg
    13021 gcctcccccg accacgacga cgtcatattc ttcgcgcacg ggaaccctcc gggcctacag
    13081 cacgagatgg atgggcgact ccaggaggtc ccggagggcc gccaggaagg ccgacgactc
    13141 ggcgccgtcc agtgccctat ggtcgaaggt aaaacagagg ttaaggactt tccggacgac
    13201 aggacgtccg tcgggatcca gggccagacg ttcttcgacg gccccgaccc ccaggatgcc
    13261 cacctggggc gggtttatga tgggagtgaa ggccgagatg ccgtacatgc cgatgttgct
    13321 gaccgtgaag gtcccgccct cgaattcctc cgggcggagg cggcgcgtgc gggcacgttc
    13381 cgccaggtcc cgggaccgcc gggactgctc gggcagggac agccggtccg cgtcccggag
    13441 cacgggaacc aggaggcccg tctccgtggc cacggcgatg ccgatgtcga tgttcccctt
    13501 gaggatcaga ccgtccccgt cgacaaccgc gttgacccgg ggattcgcgg ccagagcctt
    13561 ggtgcaggcc cggaccgtca ggtcgttgag actgatcctc aggccgccgg aggcgttgag
    13621 gctctcgcgg gcggcgagca gggacgtgac gtccgcctgt gcgaaggagg tgaccgaggg
    13681 aatttcggcg tggctgcggg ccaggcgtag gccggtgatc cgttggatgg atgtgaaggg
    13741 aacccggcta tccggccgcg ggccgtctcc ggaacggggc ctttccttta gaagccgttc
    13801 acccgtggag gcgcccgcgg cggccgcctc cacgtcggcc ttgcgaatcc ttccgccgcc
    13861 ttcccgggcg aggctcgcgg gatcgatccc ggcagcttcc gccatccggg ccgcaagggg
    13921 agtggcgtcc ttccggacgt agcggagcac gtcctccgcg gccacgggga ccccgttcgc
    13981 ggaggggacg gtacccaggt cgatcccggc ctccgaggct acgcgccggg ccgcgggtgt
    14041 cgcggccacc ctgccgccgc cgggggatgc cgaaccggtc ccggcgttga cgcccggaga
    14101 cggcgccgtc gcggcggcgg cgggctgcga cttctcggcg gaggggctct ccggaacggc
    14161 ctccccgggc ttgccgatcc aggctatgat ctgggtaacc gggacccggt cgcccgcctc
    14221 ccggaggata gcgatgaggg tgcccgcggc ttccgcctcc aggtccagga cggccttgtc
    14281 ggtctcgatg gtcgcgatgg cctccccggg ggagaccgtc tcgcccacct tcttgagcca
    14341 ggagacgatg atcccttcct ccatggccat cccggacttg ggcatgatca cgctctgggc
    14401 catgacgcct ccctccgggc aagccgaaga atctcctcgg ttatgcccgg gacccggggc
    14461 acgatgctgt cctccaggtt gcggttgtag ggtatgggcg tatcctcccc gcagagccgc
    14521 cgcaccggag cgtccagcca gaagaacgcc tcgctgtccg cgatcgtggc cgcgatctcc
    14581 cccccgacac cgccggtccg gcaggcctcg tggacgatga gcacccggcc gcacttcttg
    14641 actgtccgga tgatggcctc gcggtccatg ggggccaggg tacggagatc caggacctcc
    14701 gcctcgatcc cctccgccgc ggcggcctgg gccgcctcga ggcaccgggc caccatccgg
    14761 ccgtaggtga tgacggcgac gtcccggcct tcccggcgga ccgcggcctg cccgaagggt
    14821 acggtgtagt ctccttccgg gacttctcct ttggtccggt acaggagctt ctgctccagg
    14881 aagaggacgg gattcggatc ccggatcgcg gagatgagca gacccttggc gtccgccggc
    14941 gtcgagggca ccgctacctt gatgcccggg gtatgcacga accaggcctc caggctctgg
    15001 gagtgctgtt ccgcggcacc cgtgccggac cccgagggag tccggatcac caggggcacc
    15061 gagaactggc cgccgaactg gaaccgggcc ttggccgcct ggttcacgat ctggtccatg
    15121 gcgacggcga cgaaatcgga gaacatgatc tccaccaccg gtcggaggcc cgccatcgcg
    15181 gcccccaccc cgcagcccac gatcgcggtc tccgagatgg gggtgtcccg gacccttttg
    15241 gggccgaact ccgcgagcat gcccttggaa accccgaagg cgcccccgta gacgccgata
    15301 tcctcgccga tgaagaacac ggattcgtcc cgtcgcattt cctccgagag ggcttcccgg
    15361 atggcttcgg cgtaggtcat ctcacgcatc gcttctcctt gaatgctagg ccagcacgtc
    15421 ctgaagggct tcctcgacct tcggctccgg gctcgactcc gcgtactcca cggcctccgc
    15481 gacatcccgg gcggcttggg catccagggc gtccaagtcc gaggccagga attcaccggc
    15541 ctcgaccaag cggttccgga aaaggagcag ggggtccttt ttcttccagg cctccacctc
    15601 atccctcgtc cggtaagtct ggggatcgct cttggaatgc cccatgatcc ggtaggtatt
    15661 gagcaccatg agctcgggcc cgttctccag aatcctcttc ttcgcctcga gggtggccgt
    15721 atagaccgcc acgacgtcgt tcccgtccag ggaaaacccg ggaatgccgt aggactttgc
    15781 ccgtaccgaa atatccttga tgttcatgtg gaggctgaga tgggtggaca tggcgtaata
    15841 gttgttcacg cagacgaaaa ggatgggaag cttccagacc gaggccaggt tcagcgcttc
    15901 gtggaaggct ccttcgttcg ccgcgccatc cccgaagaag cagaccgtga tcgcgtcctg
    15961 tccgcggtag ttttgggcga aggccgcgcc aacggcgatg gggatgccgc ccccgacgat
    16021 cccgttcgcg cccaggctgc ctacggaggc gtcggcgatg tgcatgcacc cgccccggcc
    16081 tcggcaatat cccgtctctt tgcccaggaa ctccgccatc atacgccgaa tgtccatgcc
    16141 ctttccgatg gcctggttgt ggccccggtg ggtctgggcg atcaggtctt ccggcgcaag
    16201 ggcggaacag actcctaccc ccgtcgcctc ttccccgatg cagaagtggg cggtcccgtg
    16261 cacctttcct tcggtaaaca accgtgccgc tgtctcttcg aaacggcgcg tttgagccat
    16321 cttgagatag agttccctca gggtctcacg gggaaccccc atcgttcccc ccgggtgcct
    16381 gggcttggcg gcggagatct gcttcccggg aacaccccgc ggtgccttgg cacggccggc
    16441 cggattccgc gttccggggg tttcggctgt aggattgcgc tgtaacataa gttagatttt
    16501 ataccttgac ttcactttcg tcaacataat ccaacaaaag aaaatcattc aatttcgatt
    16561 gacaaatacc aagactccat attaatccta ttgtagcgcc gaatcccgga ctttccggaa
    16621 agccctcggc tccgagatcc gaacccgggg ttcttgcgcg cccaaggacg tccaccagag
    16681 tacgacggtt gtcaaccgct cgcgtcccaa actggaattt cccggaggaa tcaacgacat
    16741 gtatcccttc gaattcgaca tgacgaccaa ggcgatcttc ggcgaaggag ccgtggcccg
    16801 ggtcggcgaa ctggccgcgg gattcggcaa gaaggccctc ctgatcacct acgacgagaa
    16861 attcgtcaaa gaagtcggtt tctaccagaa ggtcgccgac agctgcaagg ccgcgggggt
    16921 gacgctcctg tccgccttcg gcgtgaagag caatcccacc gtcgagcacg ccgccggagt
    16981 catcgccctg gccaagaagg agaagcccga cgtcctcatc gccctgggcg gcggcagcgc
    17041 catggacgag gccaagttcg tgggcgtagc cgcgggctac gacggggatc cctgggactt
    17101 cgccacaggg aagaccccga tcaccaagac cctcccgctc atagcggtgg tcaccatccc
    17161 ggcgaccagc tcggaactga acgggacctc ggtcatgtcc tacgaaaaga tccagcgcaa
    17221 ggacggattc ctgagccccc tcatgcgtcc gaaattcgcg gtcctggacc cggagctgac
    17281 ctacacgatc ccgctcaagc agacggccta ttccgccgcg gacatcgtat cccacctgct
    17341 ggaggcctac atcggccacg gcctggattg ggctcctttc caggagcact attgccaggg
    17401 atcgatccgg accatcatgg aatgcatgga ccggatcctg gcgaatcctc gggacaagga
    17461 agctcgggcc cagatgatgt ggacggcctc gtacgcctgg aacgggttct atccctgcgg
    17521 cctcggcccc gcggacgcaa ccatacacgt tttgggccat tccctcagca acttctacga
    17581 taccccccac ggagcggcca tgtccgtgac catccccgcg accatgcgct acttcctgaa
    17641 tacgaagacc aagcgactgg ccggcatcgc ccgggaagtc ttcgccgtgc gggaagccga
    17701 cgacttggcc gccgctaagg ccggaattgc cgccatcgtt gcgtggttca agaaaatcgg
    17761 aacccctacc accctggggg aggccggaat tccgaacgaa gccatcgcca agatggctcc
    17821 ggacgcactc aagaccgccc aggcctgggg cctgggagat ctctactcgc tggagacctg
    17881 cgtgaagatg ttcgagctct gcaaatgatt cgttatccgg gacgccctcc ggggcgcccc
    17941 ggataacgga ccggcccgag ccttcgaccg ccgctacctg ccccgaaacc gcccgccccc
    18001 gtatccccgc cccgggatcc ctcagtacct tctacccatg cccgacctgg tgacctccgt
    18061 ctcccgacgc atgctcgaca tcccccggga ggactgggac gccctggcgg ccccggccga
    18121 gactcccctc ctctcctggg gcttcctggc cctgctggag gaatcgggct cgatccggcc
    18181 ggagacgggg tggaaagccg cccacgtcct ccttcgggac gggaaaaccg gacgtccggc
    18241 cgcggcggcg cccttctacg tgcgcgggga ctcctggggc gagttcgtct tcgaccaggg
    18301 cttcgcccgg gctgcggaac gggccggcta cccgtggtac cccaagctct ccggggtggt
    18361 tcccgtcacc ccggcgcccg cctggcgggt cctgaccgcc gccggagggg acgggctgga
    18421 tgctgcgacc cagaccggcc ggctcctgga tgccgccgag ggcctggccc gggagggcgg
    18481 cctggcggga gtccacgtcc tctggccgga cgaaccggcc gccggcctcc tccgctcccg
    18541 gccgggttgg acggcctggg agcaccaagc cttcctctgg accgacgagg ggtacgggga
    18601 cttccggggc tggctggact cgttctccaa gaacatgcgc cgcaacgtcc tccgggagcg
    18661 ggcggccctg gcggcctcgg gggtacgaac ccggatcgtg ggcgcccggg aggccgcggc
    18721 cgatccgggt atcctggccc tcatggcgga cctctacgaa gcccacaacg accgcttcgg
    18781 tccctgggcc gcacggttcc tgacccggga cttcttcctg cgcctgccgg aattcctgcc
    18841 cgagggctgg atcctcggcc gggcggaccg gggcggacat accgtgggtc tgtccttcct
    18901 gttcgaaggg cgggaccgac tgtacggacg ctactacggc gcctccgagg atatcccctt
    18961 cctccacttc gagctctgct actacctgcc cgtggaatac gccctgtccc ggggcctccg
    19021 cgccttcgac cccggcatgg gcggggagca caaggcccgg aggggattcc gctcctacac
    19081 gaccccgtcc ttccaccggc ccttcgcccg gcctgtggcc cgcttcctgg aaggcatcct
    19141 gccggagttg ggccgggaag aggccgccgc ggcggccgcg ctaaacctgg atctccccta
    19201 caaggtatga gcggggatcg acggcctcac cagtccagaa gaccgaggag ccgtgcctcc
    19261 cgaacggcct cggtccggcg cccgaccccg agcgcccggt acagggagcc cgcatggaat
    19321 ttcaccgtcg ccaggctgag tcccaggcgg tcggcgatct cctcgttgct ccacccgtcc
    19381 gccaggtatt ccaggatctc ccgttcccgg ggactcaggg agggcgcccc gaatacgggt
    19441 gtcaccggac cccggaagcc gggacgggaa acggggacca ggtccgcgcc ggcgggatcg
    19501 tcggtccatt cccagcggcc ttcctccagg gccctgcgct cctccgtgtc catgcccccc
    19561 gggtataccc ggagcatccc gacggacccc gcgttcccgt tattcattca ggaccggggc
    19621 gaagggagga cttggagggc gagccgttcc gagccccgca gtatgtccag gattacgggc
    19681 cttcccaccc gcaggggcgg aatcccgtcg tccccgagac cggccccgtc ggcgcccagg
    19741 atcaggtccc cgggctgcac tccggccgcc tccgcggggc cgccgggcgc cgtttcgcgc
    19801 accatcaggg cggtctccct gccggccgac gaagactggg ccttggaaag ccggaccgtg
    19861 ccgatggaga tcccaagcca gccccggata cgggtgcctt ccttcgccag ctcgtccacc
    19921 gctaccctcc atggccccgc gggcaggacc catccgtcgt tgccgtcctc gttggagatg
    19981 accacgccac gcagggttcc ggccagatcc accaccggcg cccccagaaa acccggatag
    20041 gcggggccgt ccacctggaa atgggcgctc gcctttcgcc gcccccattc cgtggagccg
    20101 cccgagaacc tcaccaccga aaaagccgcc tcgattccct ggaacgacgg gtaggccagc
    20161 ctcaggaccg gcgttcccac ccggggagca tcgagacctt ccgtgcccgc tcctgccggc
    20221 accggggccg cgccgtcacc gatccggatc aacacgagac cgctctctcc gtcccaggct
    20281 tccacccgcc cggtcgccgg acccgaggcc gtcacgacag gcacctcctc gccgtcccgg
    20341 gcggaacgcg ccagggaggc cgcgaggcct tcctttacca cgatgcccgt gcgatacggg
    20401 atcccgggcc ctccgatatg caccagggat cccttccagg attccaccac ctctcgcagg
    20461 gttccctcaa attccttcgc gaactcgccc acgtcgacct cccgggcttg gcgcccgggt
    20521 agaacatact gaggccccgg gccgccgtca cctggccgaa ggtatagcct cctggtgttg
    20581 cccgacgccc taggacgtgc ctacctttca ttccatggat cgggacgagg atttgctgga
    20641 ttcctattcg aagaccgtct cgggggtggc cgaatcggcc cgggactcgg tcgcccacgt
    20701 gcgagtgcgc cggggccggg atcctgaagc cgggggctcc gccttcgcct tcactccgga
    20761 cgggttccta ctcacgaaca gccacgtcct gcgggatgcg gaggcggtgg aagctgtgct
    20821 gcccggaggg cgccgctgcg aggccgcgat cgtgggcgac gatccggcca cggacctggc
    20881 ggtgctccgg atatacgggg agtccctgcc tcccctggag ttcggcgact cctcccggat
    20941 ccgggtcggc cagatcgccg tggccatcgg gaatcccctg ggtttcgaga gcaccctgac
    21001 cgcgggggtg gtgagcgccc tgggcaggtc cctgcgctcc gtgacgggac ggctcataga
    21061 cagcgtcatt cagaccgacg cggccctgaa tccgggcaac tccgggggcc ccctcctgga
    21121 ttcccggggc cgggtcatcg gcgtgaacac cgcgatcatc gcccaggccc aggggctctg
    21181 cttcgcgatc ccctcggaca cagcaaaggc tgtcgcggcg gccctgatcc gggatgggcg
    21241 gatccttcgc ggttggctgg gcatcggagg ccagggggtt ccgatacccc gaaagcttgc
    21301 ccaccattac ggtctggagg gacccgaggg ggccttcctc tccgcggtgg agcccgggag
    21361 ccccgcggac cgtgcgggac tcaaggcggg ggacatcctg gtccggttcg gcagggagcg
    21421 gatacagggc ctggacgacc ttttacgcct gctgggaccg gacagtccgg gccgggaatt
    21481 ccccgtggag gcgctgcgag gacccgaccg cctccttgcc tgggtgatcc ccgtccccgc
    21541 gccgtgacgg ttcgcccccc ctgaaagagg ggacccgccc gttcaggggg cttcagaact
    21601 ttcccaggcg gtggccgtcc aacagggtgg ccctctcccc ggtgatctcc acgccctccc
    21661 ggccggcgag ctccggaagg agtgcgtcgg aggcccagac ctccccgaga cgcagggtgt
    21721 tccgtatccg caggatccgg gcccggaaaa ggtcccggac ggaggccccc cgcagggcga
    21781 tctccacgac ggcccggtcg ctctccgcgg acaggggtat ctttccccgt tccaggaatc
    21841 cgctggtcac cacgttctcg aaggtagccc cgaggtcgat cctgtcccgg agccgccggg
    21901 tgatcacgtc cgccaggccc acccccaggg cgttgccgtg ggaagcctcg gtcaggtcga
    21961 agacggcgac ggaggagatc cggggccgat ccggctcccc ctggtcccgg acccgcatgc
    22021 ggccgatgac gttggagtcc agacccaccc cgctcacatc cttgcccatc tcgtccacca
    22081 ggagcacgtc caggtcgtcc acgggcaggc ggggctggag ctcccgggcc cgctcgagaa
    22141 gctctcggtc cgcttccgga agtccctctc cggagaccag ccggaggatc tcgggttctt
    22201 cccgggcgtt ctccacgatc cccaggcccc ccaggacctt tccggttccc agcacgaccc
    22261 gggcggcggg gaggatccgt tcccgcagtc ccgccacgcc gtgggagtgg atctccaggg
    22321 cctgggcgtg cttgcccagg ccgatgacgg ccatcttcac gaggccgctc tcgtggattc
    22381 cgtggaagtc cgtgtggggc ttcacgcggt tgaccaggag gaccccgtcg gacccccagg
    22441 cgtcccggga catgtacacc cgggtcccgt cgggggcggc gggcagttcc acgatgtcca
    22501 gggaggagac gacgggcgcc ccgaccccgg cctcggtgat cccgtagccc gccaggacct
    22561 ccctctggcc ctccgccgtg gccccgccgt ggcttcccat ggccgggatg acgaagggca
    22621 gggctccccg ggccgagagc tcccggacga ccgcccggac gatgcgggcg aggttcgcga
    22681 tcccccggct ccccacggcc acggcgatcc gtgcgcccgg ccggataccc cggaacgggg
    22741 cgccgggccg ggagaactcc tcggccacgg cggcctcgat gtccgggatg ccggcccccg
    22801 ggaagtcccg tacgaccttg gtcagggaaa gcccgtccag gttcgcatac ccgatcatgc
    22861 cgtcgccctc agaagatcgc ggacccgccg gagagcatcc aggcggccag gatcccgacg
    22921 gtcagggacc ccacgtacac gaatccggtc cgccggaaca tcgcggcggc caggaagaac
    22981 atcagggcga actgggggat cgtgaggagg aggatggaca tgaagggccc cccgaacagg
    23041 ggggtgagaa gaaggtccgc cccggggccg atgcccagga agaaggggac gtactcgatg
    23101 aaggcccaga cgagcaggct ccccagcatg accccgcagg cggtcagcca ccaggccgcc
    23161 tgggtcgccg cgggggaggc acgctccggc aggcggagtt ggccgaagag cttgaccccg
    23221 ccgttcacga aaaagaaggc cccgtagaag ggcaggtaga cccagaattg cccgaagcgc
    23281 tcgggggtga agacccggaa gaagggccag atgaaccgga gttctagtcc gaagagggcg
    23341 tcgtacaggg cggtctgggc atagagggac cccgccagga ggaaggccag gaggacggat
    23401 ttcccgacca cgggccacgg gaccgcggcg ggccgttccg ccggggccag ggccaggtcg
    23461 tggagggtcg cgcccgcggc ccggcccggg cccttccgga accatcggac caggagcacc
    23521 agggagacga ccatcaggaa ggtcaaccac acgaggaagc cgttcccgac ggtcatccgg
    23581 aataccgact ccggaagggg cgccagtccg tggcccagct gggtcaggaa ggggaaggtc
    23641 gcggctccca gaagcacagt ccccgcggca gcccgcctcc aggcccggcc ctccaggagg
    23701 acccgggacg gcaggggctg ggccgccccg gcgaataagg gaatccggag caggatcgaa
    23761 agcaggggca ggagggaggc cagggccgag agcatcgcca ggccgacgag cagctccttc
    23821 cggaggtagg tccggtccga ggccggaagg gcgggctcca aatcgaagga ccgggcgaac
    23881 cagtccaccg ccgcggcgat tccttttccg ttgtgggtca ccaggcggtg gttcgtgtac
    23941 aggagctcca tccggcgggc ggagccgtcc gcgaaggagc cgtaggtccg gtcccattct
    24001 acggggcctt cctggcccat gaagacgcgg taccggaggt ccgtcgcctc caggccctta
    24061 acggtattcc ggtagtcccg gaagaatgcg aactcgtccc agaccgcctg cagcaggagc
    24121 acgttgttga accgcaccgc ggaggggtcg tagaaaccct ccgggatgat ctcgccgcac
    24181 tgaaggacca cggccctatg gtccgggaag tccgcggcca cggaccagga agcccaggtt
    24241 cccatggagt gccccgtgac gcccagggcc gcggggtcca cgaagtcccg ggagcgcagc
    24301 caggcgtagg ccgccttacc tcccatggaa gtgtcggcca ggccctccga gtacttcggg
    24361 aggaaaaaat ccagggcgct gaagttcatc atcaccttgt agcgctcggg cccggagatc
    24421 ctccgctcga agttcgggta cttgtagggt gtgaagcccc gagcccgcat cccgggctcg
    24481 gcactcccgt ggccgaaggc gtcgatggac agggccgcga tcccccggcg ggccagctcc
    24541 agggcgaagg cggaagaagt ttccttatcg ttctggtacc cgtggatcag gacgaccccg
    24601 ggcaccggag agccggggcg ggcggaggcg ggacggtaga gcttggccgt aaccgtcccc
    24661 gcaggggcct cgatccgcac gggcgagact tcgacggttc cggaatccga ctggatccag
    24721 gaagccaagt acagggaacc ggccgacgcc gtccagaaca ggagcagggc gcccagctgg
    24781 gtacggatcg aggttttcaa acgccacccc cgggggacaa gatgggccag cctacgacgc
    24841 ggccggcggg gcgtcaatac ggggcgcggc ccacccggac tcgcggcgcc cggctcgcga
    24901 ctccgccccg tacgaacacc ttgcccgtcc cgccccgacg ggccatactc ccctcgatga
    24961 actacctctt ccactactac gccgtccgct ggctggcccg ggaggccggc gtgccggagc
    25021 ccgaggccga gactatcgcc cgttcctccc agttcgtgga cgaggctgtg cggcccctgc
    25081 gcgtcgagac cggcggggta ccctacgacc tccgggttac ccaggactac ctcttctggg
    25141 acgagtcgat cctctcggag gtctaccttc ctttccattt ccttcccggg gaccccgagg
    25201 aggcgggccg gaagcgaagg gacggggccc ggaaccccgg ggcggtcacc ccgaacggag
    25261 gggtcgcccg ggagctcctg gtggaagccc tgaagaccgg cgatccgtac cggatcggca
    25321 tcgccctgca ctccttcgcg gacgggtggg cccaccagaa tttcacggcc cggtgggagg
    25381 acttcaacgc ccttgacggc tccggcgccc tgcccccggt ggggcacctc caggccctga
    25441 ccaacccgga caacccgtcc cgtctctggg cggaccctcg gctcctgccg gaactctacc
    25501 ggatcgacaa cgcggcccgc ttcctggagg cggcgcggaa gatcttccgc tacctgcgga
    25561 cctggctgcg caagcccttc ggggacgagg atctcctcct gcccaagctg gaaggcctcc
    25621 tgcgttcccc gggggaccgg gagggggtgg aggcggcctt catcgtggac ggggacctgc
    25681 ccaagtacga gaagggcgcc tgggcccggg aggccggcgt cctggacggc tgggaccggg
    25741 gagagggctc ccggggctac gacaagctcc tgtggctgaa gagccaggcc gagcggcgcc
    25801 tgggctccgg agtccggacc gtccgcgccg gcccgggatt cttcgaatcg gacttgtacc
    25861 gctggcacga ggccgcgggg gagcaccgca gggcggcgaa ggccgccctg gccaacttct
    25921 aacggcaggg ccgccatgcc taacggcagg gccgccatgc ctaacggcat ggcttgatcc
    25981 ggccttcctc tcccgtatcc aacatgccca gcacggccat ggccgcctcc acggctgccc
    26041 ggtgaagctc catccgccgg gcggcgggca ccgtgtcggg tatctccacg atcagggcgg
    26101 gtacatccag gagccgggaa gtctccgcgg ccaggctgcc cccgggggga cccccggtgt
    26161 acgaaaactc ctccatcccg ggccggtcca gcatggccaa ggcgagctcc gccgcgcgcg
    26221 gcagggccgg cacaaccagg aaggggccgg gaccctccgt ccaggtcccg tcgctctcgt
    26281 ggaggtccag gaccaggtcg ggccgggcgt tccggatcag acggaagagt tcgaacgcga
    26341 gacgctccgc gggagctccg aggcgccggc ccgggaaggc ccggttgagg tccccggccc
    26401 ccggggccgt ccgggtttcc gcttccaatg ccgccggatc ggcctccggc aggacgagga
    26461 gccggccccg gagggcgggt ccccggtccg ccagggcccg ggccgcccgg acaccggagg
    26521 gctcgtcccc gtggatcccc gcgaccacca ggagggtcgg ccctacgacc ccgctctcca
    26581 ggcggaccgg ggacaggacg gcgcaggagc cgaggactgc gcaggccgcg gccgcgacgg
    26641 ccgcccgggc cggccccggc ggccgggcgg cgcccgggcc gcgccggaag cacgggaagt
    26701 tcacggcaga ggtttcagga acgcgcccag gcccttctcg agcagggccc cgtattccgg
    26761 cacaccggcg aactccaggg gccggtccgg ccgctccgag gaaagggcga aggcgaactc
    26821 ggaaatgccg gacaggtgcc gccccacccg ctcctcgatg gggtggccgt tctcgtcgaa
    26881 gggcacgtag agcctgccca gggcctgggc cttcacgtag taggggtccc ggcctttcag
    26941 gaccagggcc tcgtccgtcc ggccccggag ccgtccctgg gaggggttcg cggtctccat
    27001 caggaccgcc agggtatccg tggcgtcccc cagttcccgg tgggtcaggc cccgcaggtt
    27061 cttcggagag ggctccaggc cgatcttgat gtccaggaac tccaggccca ggacgacctg
    27121 ggaggccagg ggcatggccc gctcgtgggc cacgatggcg ttgatcaccg ggtactccgg
    27181 ggaggcctcg tggaggtcca cgctgatgtc gatcttctcc gcccggatca ggttcgcgat
    27241 gcccaggcag acccgctcgg tcagggtccc gtccgcccgg cccgggtaac ctcggttcag
    27301 gttccgggtc tcgttgcccg agagggtctg gccgctggcg gcatggacgt agacgtccgg
    27361 gtccggccac tggtgcacgg ggttcgtggc ccgggacccg taccggaagg tccgcacgga
    27421 cccgccgggc agggcgacgg agtacctctc gggagccccc tcctgggggt cgttgtgggt
    27481 gaaccccgag gcgttggccc gggggatcac gtacagggtc cccgccgaaa ccaaggcgtt
    27541 ctcgatgagc aggaccgcgg acaggaagga ggagggctcg ttcgggtggg tgccccccag
    27601 gacgagcatg gaggaacccg gagaggctcc ccggaacacg tagacctccg tgtccccggg
    27661 agagccggcc aggtccggca gccattcgga gagcatgcgg acctcggtga cgcccggacc
    27721 ccggacgatg ggctcgggct tacggtggtc caggaagcgg atcgaggcgg ccgcggcggc
    27781 ggccaaagcc aggagggtga ccgcgagggc ggtcgcatgg tgtcgtttca cggcattccc
    27841 ctacttaatc aggaggaccc ggagcaggag gggaatcgcg accatggccg cggtggcctg
    27901 cttccgccag tccttctcgg tccagaggtt ccacgcggtc agtcccagga cggatgccgc
    27961 gatcgccagg tacaggtatg tcatctcttc gctcctgtcg tcagtagaga gcccggatct
    28021 gcttggagaa cacgatgaac gccagcgccc agccgatgat gaccagggag gggacgacca
    28081 gcttgaccag gacccgggaa tacttcgatt cccccaccac ctgggccgcg aagatggcgg
    28141 acagggccgt ggggggcatg aagtccccca gggaggcgat gagggagagg gccgcggcgg
    28201 tcacgatctg atcccggccc aggagggcca ggaggaaggg aacccccagg acgctggagg
    28261 ctccgaaggc cgataccgcc ccgaagaggg gtatggacac ggcgatggcc gcgtagagca
    28321 gggccggggg caccgagagg gcgctgacca ccacgaaacc ccggaccccg gtcagggtca
    28381 tcacctggat gaacatcccc acgccggcca ggatgcccag gaccggcagg gcgtcgttta
    28441 ccgcggcctt ggaggccgaa aggatgttga agcgctttcc gaccacgagc cccagggcgg
    28501 ctccgatcag gaagaccacg ggaagcccca ttccgaagac ctgcggcagg gtctttccca
    28561 ggaccatcag gacggcgacg accaggatgg gcaggaagag gcggatgccg tatttcttgt
    28621 acgtctccag gtccagcttg gcggccagga cctcccactc cacgaccttg acgtggggaa
    28681 gcccgaacca gagggagaac aggacggcca aggggaaggt caggagggcc aggggaacct
    28741 cgaagcccac gtagggcatg tcgatgcccc cgcctatgat catggcgggg atgttcaccg
    28801 ggggagcgat catccccagg atgcccgcca tggctatgaa ggcccccgtc acgggggcgg
    28861 gcagacccag gaggatcagg accggggcga ccacggcccc cgcggtcagg accgaggcgc
    28921 tggaactgcc cgtgatcatc cccgggaaca tggccaccag catgagcagg ggcaggagga
    28981 gggccgggac ggtccggaag cgccggagga tcacggcgtt cagggcttcc agggccccgg
    29041 attcctggac ggtccgcatg aagaccatgg ccgtggcgat gaccaggatg gtgtccacgt
    29101 acccgaaggt cccctccacc aggtggcgga cgggcaggcc gttcccggag accaggaggc
    29161 ccgcgcaggc ggccaggagc atggacaggg agaccgggag cttcgcggcg aaacagcccg
    29221 ccgcgaagac cccgaccatg gctaggaata tgaccagttc gaagggcacg gcgtccccct
    29281 acttgaacgc ggcggccagg ggcacccccg cggcggtgat cttgtccacc ttgaccaggg
    29341 gaatcttggc ctgggaggcg agcttggtaa agaggccgtc cttgtccgcg tcctccacca
    29401 cgatggcgta gtccgagacc gggacgcaga ggctgatgaa ggcgtccgag agggtgcccc
    29461 gccgggcctc gccgcccacg tggatcgcga tgatcttcat gccgagctcc cgggcccgct
    29521 tgatcagggc cttggcccgg tcgatctccg cctcggtgct gatgcccgcg gctcccaggc
    29581 ccttggagct gccgcccacg gccaggacca gggtcttggc gcccccgggg agcttggcgg
    29641 cggtgaccag gggatccgcg gtgtgggcga ccttgccccg attgaggagg accttgacca
    29701 tctcgatgtc ggcgctttgg ccgacggagg tcaggagggc gggcttttcc gcgaaggccg
    29761 cgggagcctg ggcggacagg gccgccgcgg cggccaaaag gacgaatacg ctgaggattg
    29821 cacgacgcat agaaactcct ggaagcggac gggccgccgg ttcctccccg ggggaaggtc
    29881 cggcggccgt tgcgaagcgg cggaggcggg cccggcgggg tacgccgggt cgtccgccgc
    29941 gtatggatca gaacgccgcg gcctggccgt cccgccgggg gtcggcgccg ccgtagagga
    30001 ccttggcctt gcggtcgaag agcaccgcgt gcacgccgcc gaagtaggcg tcccagtcgg
    30061 cccggacgtt cacgttatgc cccagggcct cgaggccctt gagggtgttg atggaagccc
    30121 gtccctcgag ctggacctgg ccggaggcca tggcgaagac ccggggggcc atgatggaat
    30181 cctggacgga catcctgtgg tcgatcacgt tggagatgac ctgggccacc gccgcgatga
    30241 tccgggtggc tccgggggag cccacggtca tgaagggctg accgtcgggg tccaggacca
    30301 gggtgggggt catggaggac aggggccgct ttccgggctc cacggactgg acgtggccgg
    30361 gcttcagggc gaagtcgtcc atgtggtcgt tcaggatgaa tcccgtgccg gggaccgtca
    30421 cgccggagcc gaagaagtag ttgatgctct tggtgaccgc caccatgttc ccgtccttgt
    30481 ccatgacgga gaagtgggtg gtggagccgc tctcgaactt ggtcggatca tcggcggtgg
    30541 cggagcccat ggccttggcc atgtcgatct tggcggccag gacctttgcg tagtccttgt
    30601 tcgccaggcc cttgaggggc accttcacga agtcggcgtc ggccatgtac ttgccgcggt
    30661 ccgcgaaagc caggcgcagg gcttcgatcc acgcgtgggt ggtggcgggg gtgccgggcc
    30721 ccatcttggc gatgtccatg ttctccagga tgttgaggat ctggatcacg tgggttccgc
    30781 cggagctggc ggggggcacg gagacgatcg tgtatccgcg gtaggtgccc gtgacgggct
    30841 tgcggatctt cacctggtag ttggccaggt ccgccgcggt caggatgccg cccgcgtccc
    30901 gggcggcctt ggcgatccgc tcggccatct cgcccttgta gatcgcgtcc ttcccgtcct
    30961 tggcgatggc ccggagggtg gcggccaggt ccggattctt gatggtctcc ccgagctcgt
    31021 aagggagtcc gtccttggtg tagatcttgg ccgtggcggg gtacttgttg atcttgccca
    31081 actcggactg gatgatcgag gccaggttga cggtaacggg aatgcccttc tccgcccact
    31141 cgacggcggg ggccaggacc ttctcccggt tcatcttctt ggaaccgtag ttctccaggg
    31201 cgtacaaaag tcctgcgacc tcgccgggaa ctccgacgga caggccgccc acgacgctgg
    31261 cgttgtcgat gaccttgccg tcctccccga acttgaacat gtccggtttg gccgccgcgg
    31321 gggcggtctc ccggaaatcg atcaccacgg cttccttcat gttcgccatc ttgatgacca
    31381 tgaatccgcc gccgccgata ccggaggcgt tgggttccag gacacccagg gcgaaggcgg
    31441 tggcgatggc cgcgtccacg gcgttcccgc ccttcttgag gatgtcgatg cccacctggg
    31501 acgcctcggg cttggcggcc gcgacgacgc ccttggcgcc ctgggccgcc cggccgtaca
    31561 ggttgaccgg ggtctgggcc gcgatcaggg cagcggcgaa ggcgaccaac acgaataccg
    31621 agaacaactt tttcacagga tcctcccttt gtttctctgg atagaaactt tccagcgttt
    31681 cttaccgaaa ctaattaaat atttaccgat tgatccgagc ttgtcaaccg caggactccg
    31741 cttgccggtc cggacgggat tgcggcatac tgggcgggat gcgccccacc cgaacgcgcc
    31801 ccacccgccg aaacggggca cccgccttgg tcctgacggc gacactggtc ctggcctcct
    31861 gcgcgacccg gatacccgtc gcccaggagg cggacccctc cgccggactg ccccgtgaag
    31921 cctcggtcta tctccgcgcg gaccgccctc tcctcgaaga ggccgccggg gccctcctgg
    31981 cgccggcgga cctcagggcg gtccggccct tcctggcccg tacggaccgg gtcacggcgg
    32041 cgctggtcct tccgcggaac gattcgggcg gctccggtgc gggcccggga gccccggaga
    32101 cttcggaagt cttcgggatc gccgagggca cctaccccgc cggcgccgcg gccttccggc
    32161 tccggctctc ccgggagtgg aggaaggaag gccggaccct ggtccggcgg gacggaaagc
    32221 tccgcatcgc cttcgcgggc cggcaggtcc tggccgcctc cacctcggac ctggagcccc
    32281 tcctggaccg gctggccgtg cccggcccgc atcccgtccc gggggatctc cggccgtact
    32341 gggaggctcc gggcgccctg tggatccccc gacccgggga aacggcggaa cgcatcctcg
    32401 gagccggagc tccccgggtg cccgcccggg gcatgctcct gtccctgaac cccggcgaag
    32461 ccgtaaccgg gtactccgga acctggatct tcgccttcga atccgagcgg gacgcccggg
    32521 tctacgcccc catatgccgt ctggcccacc tggccttcat ccgggtcgtc cacggtacgg
    32581 ggacggaagc ggccgccgcc ctggagcgca cggtctggac cgcggaggga accctgatca
    32641 aggcctccgg gtttcccctg gacctgtcct cgctgttccg ggccttgaag gccgcgttgc
    32701 ccgagagttc ccagggttcg tgacggcggg gtcccgcccg gtcggagtcc ggaccccggc
    32761 ggggatcact caggccgggc gggaaccctc tccaagaaat cggccagggc cggaaccacc
    32821 cccggggaca ggggatacga gggatccgag aaggactcgt agttctcctc cacctcgttg
    32881 cccacgagtt tgaggatatg gttggtatcc gggagcagca ccgcttccgc gtccggcacg
    32941 gacaggcgca gtcgatcgaa ctcctccatg gtgacctgga ggtccaggtc cccctgcatg
    33001 agcaggatgg gcaggcccgc ggcggacagc cgggcgagct ccgtccccag gtcgtagcg
//
LOCUS       DAOTEI010000016        32785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_892_length_32785_cov_5.932203, whole genome shotgun sequence.
ACCESSION   DAOTEI010000016 DAOTEI010000000
VERSION     DAOTEI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 32785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32785
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_32785_cov_5.932203"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="P5313_02710"
     CDS             complement(<1..109)
                     /locus_tag="P5313_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+-transporting NADH:ubiquinone oxidoreductase
                     subunit D"
                     /protein_id="HRY79308.1"
                     /translation="MPANSLLLSSSPHIYSGVSTRRLMLSVIIALLPATV"
     gene            complement(99..1445)
                     /gene="rsxC"
                     /locus_tag="P5313_02715"
     CDS             complement(99..1445)
                     /gene="rsxC"
                     /locus_tag="P5313_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HRY79309.1"
                     /translation="MEKKTFRGGAHPPERKDRTSELPIEYVRSVKQVVVPVNQHFGPP
                     IQPLVKVGDTVKRGQKIADAEGRMTVPVHAPISGVVKKIEPRLQPNNAEGPAIVIEPD
                     GSDAVDFLPVLDAFSCTKDQALARIREAGIVGMGGAGFPAHVKLAPPPNKPIDVVIAN
                     AAECEPYLTIDERLLEERSGDVVDGMAILLKTLGVAKGVIGLEANKEHLVSLLRKTID
                     ERRREGQEISVIVLETKYPQGGEKMLITAITGKEVPSGGLPMDVGCVVQNVGTLAAVA
                     DAFNKGQPLIERGFTITGGACKTPKNLVVPIGTLVSDLVPDTVQVDDENLARVVAGGP
                     MMGPAVPSYVFPIQKNTSGVLLMDKAEARYFQEQPCIRCGRCMRACSCRLSPALLNEA
                     LNAGDLDAAEAIGVLDCIECGTCSFVCPSRIQLVQRFRIGKQLLRAKKQKEAQRAR"
     gene            complement(1911..3014)
                     /gene="corA"
                     /locus_tag="P5313_02720"
     CDS             complement(1911..3014)
                     /gene="corA"
                     /locus_tag="P5313_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRY79310.1"
                     /translation="MKDAHNRARPRPIEPIFKSVSSGTAGSVPGTPIYIGDRTPVEAT
                     LSLIQYNAEQVSFITPESLEEILAMQEEGMVNWVNVNGLADQDLVKRLCGYFRLEALT
                     VEDILNTEHRPKAEDFGTYFLLITKMITRKPDCTVEYEQVSIVITPFGVLTFQEVPGD
                     CFGPVRERLRTGVGRLRRLGPSYLAYALLDVITDNYFSVLESLGDQLETREEIAAARS
                     SGNRFMVELQGIKADLNRMRRTVWPVREAVGALLHSQSDILEDGLDPFLRDLYENTVQ
                     VLDSLEVYREAAASIQEVYLSSVSNRMNEVMKVLTIISTIFIPLTFIVGVYGMNFQYM
                     PELEQPWAYPATWGVMLAIALGMVAYFKKKKWF"
     gene            3196..4290
                     /locus_tag="P5313_02725"
     CDS             3196..4290
                     /locus_tag="P5313_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY79311.1"
                     /translation="MSVRARWSWAALGSLAFLAASCGKPDGVAGATAWEGQQTTETVA
                     VESLKVVRGSLADEIRASGTVAGIREAFVVSETQGLVKSVTFSLGDRVEEGRELLRVD
                     DRIAALNLDRSREQYEAARLELDATEKLAAAGRAAPADLTRARGNASGAKAQYETALK
                     SFNDTRLRAPVSGVIAAKDEVATVGATIAPGARVARIIDTSSFRITVGVGEREVGLIE
                     QGAAVKVYVPAALGEDFVEGTVAAVAAGADPATGSFPVVVAFRNGFAGRVKSGMAASV
                     SIAARKSEPLVVIPSAALVRRGPDFAVFVDRGGTAEVRPVTLGRRSGVRAEILSGLAE
                     GEKLIISALTRLRPGVPVVSTERGDTADWE"
     gene            4300..7380
                     /locus_tag="P5313_02730"
     CDS             4300..7380
                     /locus_tag="P5313_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY79312.1"
                     /translation="MNLPKFSTENSVLLTIVLVTVLVLGTFSIARMPQEQFSEVPFFW
                     VNIAVPYPGVSAGDIERSVVVKIEREMQGLDNLKRIQSVASDGLAIVRVEFDDGISND
                     EFDRLFQDVRTRFGRVELPDGTLQALVDDFSSNDFLPVIEVAVSGPAAYEELNRIART
                     LRDRILEVKDVSTVDLVGDRDRRIVVDADRERLEAVGISLSEVLRAVQQKNVTVPGGT
                     LETVSREYILRTVGSLQGIGDFDKVIVRRVGPSGAVVHVGDVARVYEAFDESGDTARI
                     NGERAVILRVGKVPRGSAIGVIDGVKKIVEETWPGLPEGVRIDLVNDSTVQIRDSVDV
                     LVSNAVQGLILLSVILLLFLGVRNALMTALGIPVTFAITFVILEAMGETLNSNTLFGL
                     VLVLGMIVDHAIVITENTYRLRYMGYDKKRAAIEGTAQVAWPVVSSSLTTVAAFLPLM
                     LLPGTIGKFLRVIPLTVIIALLASTLEAILFIPSHAAEWPGGDRLSSKPDFFQRIRPG
                     FERFVKGLYGRRRLVVLGFAVVTVAIFSTIPFIRQDLFSAEDFTLFYIDLDMPAGTNR
                     EATERTIRAFEERILPLKGNGEVANVISSVGFRSDGSGGTSAGNVGQIVVDLTEVREG
                     RKRSVTAVMDEIKGMTSDIPGPENVLYRKAQSGPPVSPPVGFRIRGDDYDGLIETAAL
                     MRERLAGYPELFNIEDTLQSGTPELRVRINEERAASYGLSVAAVGSFIRGSYDGLPAG
                     TVFFRNEELDVIVRYEGTGGASSVERLRELKIPAADGRLVPFSAVAELVEGAALASIK
                     RVDGKREVEVASQAYDKKGIQGINADIRAWFRTEVASRHPDQTLIVGGEFAEFADLLV
                     QILRVFLIGLFLIYAVLGAQFKSYTQPFLILLSVPIAFSGVILYLVFSGTPFSTTVLY
                     AGVALAGISVNDSIVLIDFINEDRRNGKPVAEAVVDAAVTRLRPIFLTSVTTIAGLLP
                     TALGLGGYSVVWSPMASTIIFGLIFSTTTAVTVVPALYGLLFDRRPRVAKGAAV"
     gene            7404..8906
                     /locus_tag="P5313_02735"
     CDS             7404..8906
                     /locus_tag="P5313_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY79313.1"
                     /translation="MNPAFLRAAAAAFAALAAILPAAAQAAGPGPTGSPLTLSQVAVR
                     ALENDPAVAASRAAAQTARVQYELKLDEARPRFILGLTPFSLDQRRIFDFTGFDYADA
                     RTLSAGGGLTLNQALPTAGTLSAGAKAAFKAIDTDGTLEYELTPSLSANLRQPLGAGG
                     QFLPVAAAAGAKRSAELASDQAALDDLGRRNQAVRGAVDLAGRVLVLRAVLDTQTAGL
                     ETARRRAESALLRRRGGTATEDAALELELAAETLRAAREETRLSLRDAERRLAGLLGL
                     AEAPALSGRLPALAPAPAPEPRTAPEAARAALALERAAADAAARAVSDAASFGASLSL
                     EPRYPDTRADPDDLGSVLSDYFGGGTGAGMNLNLTLTLDVPLSVREARALRSRSDALA
                     ARAAAANLRAAEQTARERARALEERRAFLADRVEIQKRIADLSRRKWERLRDLAAAGT
                     VPADEADAARTDWDRAVSDVLRTEVDLVLAELDIRILRGEDLAVVLAGAK"
     gene            complement(8983..9417)
                     /locus_tag="P5313_02740"
     CDS             complement(8983..9417)
                     /locus_tag="P5313_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY79314.1"
                     /translation="MTLIKHVLRNKMTLPVTVAPGDTVYSALEAMAKHNIGAVLVMDG
                     KRLVGILSERDYARKVVLKGKLSKDTLVREIMSEKVHFATPDQSVDECLARMTHARFR
                     HMPVLDGEKNVIGIVSIGDLVKEKIAEQELVIDQLQQYITAG"
     gene            9733..11412
                     /gene="ettA"
                     /locus_tag="P5313_02745"
     CDS             9733..11412
                     /gene="ettA"
                     /locus_tag="P5313_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRY79315.1"
                     /translation="MATVDDKKIIYSMYRVSKRHGTKQVLKDISLSYFYGAKIGVIGL
                     NGSGKSSLLRILAGVDTEFAGETSVSPGYTIGFLEQEPKLQTGRTVKEIVSEGVQEIV
                     DLLKEFDEVNEAFGDPDADFDKLATRQAALQEKLDAADAWDLDSRLEFAMEALRCPPG
                     DQVVDVLSGGERRRVALCRLLLRKPDILLLDEPTNHLDAETVAWLERHLREYAGTVIA
                     VTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLDQKRQRLEQEEKGESERARTLARE
                     LEWIGMSPKGRHAKSKARISQYEKLLAEDGREKIKETRIFLPAGPRLGSVVIEAKGLS
                     KSYGDKVLFQGLEFEVPPGAVVGIVGPNGAGKTTLLRLITGEEKPDSGTLRLGETVKL
                     AYADQMRGNLDPDKSVWEQLSGGLDIVKLGGTREINSRAFCSWFNFSGADQQRKVGVL
                     SGGERNRLNLALMVQTAANVLLLDEPTNDLDVNTLRALEEALDSFAGCALVVSHDRWF
                     LDRVATHILAFEDGEVVWYDGNWTEYAEYRREKLGASADRPHRYVYRKLER"
     gene            11529..12293
                     /locus_tag="P5313_02750"
     CDS             11529..12293
                     /locus_tag="P5313_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79316.1"
                     /translation="MARYVLLALPYALQLLCIVHAMRTGRAYYWIWIVLFLPYVGGLA
                     YLIVEILPGLRLRRHLVVAQTAVDRVFVPAKKLRDLRNKASFSPTVENRIEYADALAE
                     AGAREEALAEYEACLGGLARNNPAILYKAAAAAFEAGDFERASGYLGRLPRKPDGTFE
                     QKPWNLLHLRILERTATPEAAAAGFERFCTLVNDREIDCLYLEFLERTGAYDKLETVL
                     ERIRAEEESLRGMNVRYDRALYRRAYAVGKRARRPE"
     gene            12335..14164
                     /locus_tag="P5313_02755"
     CDS             12335..14164
                     /locus_tag="P5313_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY79317.1"
                     /translation="MPAGSDAGYYTKERNPKTERTTSRKSWFPAISIRSALLLILVAA
                     LAPAFHIIAVTGAEHARHMEEQAAAEALRQAAAFGDIQTRITESTRQMLAVLAALPSF
                     RSRNDDIAERTLQAVHSGNPEYLNLSLTDGAGRVTASSLLARGTDLSIRPHVHRALTS
                     GRFSTGEYILGLVQSEPSFSYSFPIPDESGRPYATINALYKLSAYAVLFETFKLDPDS
                     FLGIVDRNGIRLFFYPPRDANPVGKPIKEEVWLRIQRGDDAGTFLDTSSDGVVRFFGY
                     RKLRLEGDPAPYITVVYAVPREKILRASRTITRRNLLAMAGAAILALALAGILSEILF
                     GRRLSRIGSAVDRIRGGDLGARVRLEGDSSDLGRISAALDGMAESVQRRDAEKEEEAL
                     RLSRLLDEKSVLLKEIHHRVKNNLQMTLSLIRLQEGASSEDGEASLKGLESRISAMSM
                     VHEMLYGSENLTDIDLGEYCGRLLDLLRSSYAGKNPLQVSLTCETVRCPLESAIPFGL
                     ITAELVTNAFKHASGRGPAAVSVVLAHKDGTACLTVSDNGPGLPPGFDPAGSRRLGLQ
                     LVLALTSQLRGTLTWENDGGARFRVDFPLDTPKGPGAPDPEAG"
     gene            14269..15942
                     /gene="asnB"
                     /locus_tag="P5313_02760"
     CDS             14269..15942
                     /gene="asnB"
                     /locus_tag="P5313_02760"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011760214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HRY79318.1"
                     /translation="MCGIFTVLDIKGNPVELRKLAINRVKRIRHRGPDWSGVYSDKRA
                     VICHERLAIVDVTHGAQPLVDKGTGRVLSVNGEIYNHLELRARYLKKAHEFQTASDCE
                     PILYLYDELGPDFVKLMNGIFAFVIYDPRDGDFFIARDHIGINPLYWGRDGEGNLFVA
                     SEMKALYDVCPKVEVFPPGHYYKGSEGRLVKWYEPAWAAPGRIPDGEADLEALRDALC
                     SAVKRQLMCDVPYGLLISGGVDSSIIAAIAAKFSEKRVESGDTERAWWPRMHSFSVGL
                     PGAPDTKYARKVAEFIGSQHHEIEFTVQEALDAIPDVIYHLETFDVTTIRAGTPMYLM
                     SRVIKSMGIKMVLSGEGSDEIFGGYLYFHKAPNPVEFHEELVRKLSLLHLYDCLRANK
                     AMTAWGVEARVPFLDPEFLDTAMNIDPKHKMIRKDEGRMEKYILRKAFDGWVPDEVLW
                     RQKEQFSDGVGYSWIDSIKAHAEKEVSDSAMANARHRFPVKTPPTKEAYWFRSIFEEH
                     FKNPSAVDLVPDGPSIACSTPTAIRWDAEWAKLADPSGRAVRGVHDKSN"
     gene            16193..16678
                     /locus_tag="P5313_02765"
     CDS             16193..16678
                     /locus_tag="P5313_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY79319.1"
                     /translation="MDRNEKEALIVRYETAFEPIRHLVKDLSAKELSFQPDLPEAWSI
                     NEHLVHLLEADLICWYRIRASIAEPGVTIPVWNQEAWRSRLRYGDLDGIGCLAEAERV
                     RALIGRTCRGALDGDWSEFHVIHPERGRMDLAQLLELYCGHGAFHLKYVERNLAACRA
                     R"
     gene            complement(16720..17037)
                     /locus_tag="P5313_02770"
     CDS             complement(16720..17037)
                     /locus_tag="P5313_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY79320.1"
                     /translation="MDQEEREKLCPRVQAAFELLGRKWTGLILHQLLEGECSFSDLER
                     TVPSLSSRMLALRIKELEAAGLVHRTVVGGPPIRVSYRLTEKGLALEPAMRGIEEWAQ
                     AWT"
     gene            17144..17767
                     /locus_tag="P5313_02775"
     CDS             17144..17767
                     /locus_tag="P5313_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRY79321.1"
                     /translation="MNDFSAAAPRERIEKAMAALRANGIDAVFARDAAAAKDTVLELI
                     PAGAEVMTMTSVTVDSIGLGKALNEGGRFDAVKPRLSSMDPKTRGREMRKYGAAPDVA
                     VGSVHALTEEGHLVIASLTGSQLPAYAYGSAKVIWVVGANKIVRDLEEGIRRVEDYVL
                     PRESARARAAYGLPDSFHSYPSKILLIRREVQPGRAVVVVVGESLGF"
     gene            17980..19308
                     /locus_tag="P5313_02780"
     CDS             17980..19308
                     /locus_tag="P5313_02780"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRY79322.1"
                     /translation="MVSENIRSAYSAILNEELVPALGCTEPIAVAYAAARAREILGRM
                     PEGLCVRCSGNIVKNVKGVRVPNTGGLKGIAAAAVAGVVGGDASRRLEVLEGVRPDQL
                     EEVRRLLAGGYCEVSLAEGVENLFIEAEAFDGDDTAVVRIEGSHTNITLASRNGESVR
                     HSVAAPEAGRKPESRGDATAGPRVEGGALASGAGLLNIRDILEYAETADLSDVERVLA
                     DQIEMNRAISDEGLNHAWGAQVGKTLLKTRGTDVRVRAAARPAAGSDARMSGCSLPVV
                     INSGSGNQGMTVSLPVVEYALELGVPRERLIRALALSNLVAIRQKIRIGKLSAYCGAV
                     SAACGSGAGIAFLRCGGYREICDTIANTLGNVGGIVCDGAKPSCAAKIATAVEAAVLA
                     QDLAFEGRAFADGEGLVSEDVERTIENIGTLGREGMRETDKVILRIMTSD"
     gene            19359..19919
                     /locus_tag="P5313_02785"
     CDS             19359..19919
                     /locus_tag="P5313_02785"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY79323.1"
                     /translation="MAKPSDWKELGSGRIADCRIFDVSSSDRACPDGKRGTFFLVNAP
                     DWAGVIPVVDTPEGRKFVMIRQFRHGTGRLSVEFPGGIVDQGEDPAQAVARELLEETG
                     YRADLVVPLGLLSPNPAFMTNTFHAFIAEGCILERPQALDEHEDIEVLLVPEHEAIDL
                     VGAEDYGHALMTATLFLYMRYRGFCP"
     gene            19933..20970
                     /locus_tag="P5313_02790"
     CDS             19933..20970
                     /locus_tag="P5313_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HRY79324.1"
                     /translation="MNVEITEPRELLDPDGRITKEGWARKPFWRYDRSRIKAPWWRIK
                     EWDYYSVLSRDGKFGITLTLSDLGYAGLCAVCFLEFERRHVCQVDTLSVLPMGRTGLG
                     PDSDAGHVAFADSKLALDFEYLPGKRILTFAAPGIRNAQGDEGLTGSVTLSQPDGLES
                     MNIATSWAENRRAFYYNRKINCMPASGWFTVGDRRYEFDPARDFGALDWGRGRWTYRN
                     RWYWGSASGHLDGRPFGWNIGYGFSDRSPASENALILDGRIHKLAEVEFRIDTSDYLK
                     PWRFTSSDGRFEMDFEPAVDRASETNLGVIRSVQHQVFGSFTGTAISDDGTKIKVKDF
                     PGFAEDVLNLW"
     gene            complement(20974..21468)
                     /locus_tag="P5313_02795"
     CDS             complement(20974..21468)
                     /locus_tag="P5313_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HRY79325.1"
                     /translation="MNAAIETIALDYGEGKNNIIVGQSHFIKTVEDLGEILAAVPGAK
                     WGLAFNEASGPCLVRTEGNDPSLVETAAACAKAVGAGHTFYVVLGPGTFPLHALDRIK
                     SCPEVCRVFCATGNPLQVLVARTPQGAGIVGVVDGSSPKGVEGEADKSDRKSLLRRFG
                     YKFA"
     gene            complement(21465..23066)
                     /locus_tag="P5313_02800"
     CDS             complement(21465..23066)
                     /locus_tag="P5313_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY79326.1"
                     /translation="MKSITAKIILLAAGAALVVTIGLSAVFGYLFVSASNDQISALEA
                     TLREDYDRLIKSQVESAVSLVERIGKMRNAGELGPEAAEKLARDLLRDLRYGKDGYFW
                     ADTPEGVNKVLLGNASEGTNRYNLKDAKGFELVKAIIGAALAGGGYTDYWFPRPGETE
                     ANLKRGYSMLAPSFNWVVGSGNYIDDIEKVVGQKRAEADARLRAAVGALVPLAALLAA
                     VMVVLAVVVGRRISAPLSYAVERVRRISEGDLTGDFDPGFLARPDEAGRIVRGLSAMR
                     DDLSRLIGQAAQASRRVALGSGQFREASNGISDGASRQAAGAQEVSASVEELSATARR
                     NAENARETAGIASEAAREAAESEDSFRGAAQALRSIADRIGVIEEIARQTNLLALNAA
                     IEAARAGESGKGFAVVAGEVRKLAERSGKAAEEIRGISADTQKQSDRALESLGRLVPA
                     IRRTSTLVEEIGAASREQEIGTEQISKAVLALDEVVQQNAAAAEELAASAASLSEESV
                     RLEKAVSSFKTEDRALALPGTEDPE"
     gene            complement(23088..23834)
                     /locus_tag="P5313_02805"
     CDS             complement(23088..23834)
                     /locus_tag="P5313_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRY79327.1"
                     /translation="MSETYETGRNFLRSNWSLIEDPWKSDQSKGVPAPAQEEGPREGE
                     RILPLVPASEFKPRGGSLLDLLRTRKSRRKYTDGYLTREEFSFLCWAAAGIKDHRPKF
                     SFRTYPSGGARHPFDLFAFAARVEGVEPGLYRYLPVEHALALVRLGDDSHALGEALCG
                     QDWNPAAVLVWAAVPYRTEWRYGPVSHKIIALDAGHACQNVYLACEAVGAGTCAIGAY
                     DQEKLDAYLGLDGRDRFALYAAPIGRFSSE"
     gene            complement(23906..24670)
                     /locus_tag="P5313_02810"
     CDS             complement(23906..24670)
                     /locus_tag="P5313_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY79328.1"
                     /translation="MANQILLGLVVTLAFVVKRLTGFGNTLITNTFFLFFTELRFAAP
                     AELLTNVPMNLAMAWKARRLVDVKFVLVLFAFVLAGILGGALILDGIRGRPLKVLLGL
                     FVAADSLIVLVRGRAPSVETTEPEERARRAPVRGPGDALRLTASGLSLGLFGVGAVLA
                     AFLARRFRDRDALRGTLCAVFFLDNVCRFGAYAALGLYGRETFRAALGLYPFVAAGFL
                     LGQVLDGRIPERVLFIGTRILLIGSGAALAAANLGG"
     gene            complement(24697..26187)
                     /gene="glpK"
                     /locus_tag="P5313_02815"
     CDS             complement(24697..26187)
                     /gene="glpK"
                     /locus_tag="P5313_02815"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRY79329.1"
                     /translation="MSRSETYVLALDQGTTSSRAIVFNRGGNIIGVRQIPFPQIYPKP
                     GWVEHDPAAIWDTQYEAAKGALAAAQVGPDQIEAVGITNQRETTLVWERATGKPVYNA
                     VVWQCRRSAEICKRLAARGLAPTVREKTGLVLDPYFSGTKLVWMFDEIPGLRTRAERG
                     ELAFGTVDSWLIYNLTGRHATDATNASRTLLFNIHTGRWDEELLAELKIPASILPEVL
                     PSSAEFGKTRRELFGREIPVTGCAGDQQAALFGHACFEPGSAKNTYGTGCFTLMNTGS
                     QPVASKHNLLTTVAWDLGKGFTYALEGSVFIAGAVIQWLRDELGLISDAAESEALAAS
                     VPDTGGVYIVPAFVGMGAPYWDPDVRGTIVGITRGTNRARLVRAALESVAFQSRDLLR
                     SMENDFGSALPYIKADGGAAANSFLMQFQADVLDRPVILPEVNEVTALGAAYLAGLKT
                     GYWTDVKEVERNWKRRREFRPEMAGETRNRLLSGWDKAVGSARSFR"
     gene            26624..27790
                     /gene="metK"
                     /locus_tag="P5313_02820"
     CDS             26624..27790
                     /gene="metK"
                     /locus_tag="P5313_02820"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRY79330.1"
                     /translation="MRDKSYLFTSESVGEGHPDKLCDQISDAVLDACLAEDPESHVAC
                     ECFATTGMVLIGGEITTETYVDIQTLARDVVREIGYTNPDYGIDYESMAILNTIHSQS
                     PDIDQGVDGRGLKEYEGQQGAGDQGMMFGFACRETPELMPAPIMYAHRILERAARLRK
                     AKEIPWLRPDAKSQVTLQYDGHKPVRADAVVVSHQHDPDIQYATIRDTVIEEIVKPVL
                     GPTGLLDSNTKYYVNPTGRFVIGGPMGDTGLTGRKIIVDSYGGMGRHGGGAFSGKDPS
                     KVDRSAAYMARYVAKNVVAAGLAERCEVQLAYAIGVPFPVSVMVDTFDTGKAAEHVIS
                     DAIRRVFDLSPAGIIRTLDLKKPIYQRTAAYGHFGRDGFAWERTDKAEELKKAL"
     gene            27813..28154
                     /locus_tag="P5313_02825"
     CDS             27813..28154
                     /locus_tag="P5313_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79331.1"
                     /translation="MAEISYTLARFLEAVRDYPAERVQARLLSASDRDLAAVLAALKP
                     PDREAVLARIGPAKRTRVEEGVERMRFVRLAPGQADRIAAHLTAHIQGDVPLGPASSH
                     FRPHKSEGERD"
     gene            28151..29389
                     /locus_tag="P5313_02830"
     CDS             28151..29389
                     /locus_tag="P5313_02830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY79332.1"
                     /translation="MNPEPEGVPDTSAEARPAPAPRRALRRGDLPDNFTRSLYGLSPY
                     RGCAHGCRYCDGRAEKYYVEGDFERDIAVRGWLPDRLAEELPRLREPGMVAIGSGTTD
                     PYQPVEAEARIVGRCAELLADAGMPVTVMTKSDLVLRDLDAWRRVNERSLFVLLISIT
                     TLREDVRAALEPGASPVKERLEAMRTFKKAGCAVGALAMPFLPGITDDEAGIRVLYTE
                     LAAIGVDFVMPGGLTLRPGRQKDLYLKTLGEYRPELLGSVRDLYREDRPSGRPIRAAE
                     ETLHRTIATVRKDVPLPFLLPHRVFSRALPAYDTFRVLLRDMAELYADRGVDTRPLRK
                     SADRYDQWLIGLRRVHRRKRSLPDSWLADRFEDALDTGELDGVLDNRRLSGFARSVLR
                     DGATLDYRTLRLVPNTLKSD"
     gene            29661..30224
                     /locus_tag="P5313_02835"
     CDS             29661..30224
                     /locus_tag="P5313_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HRY79333.1"
                     /translation="MIRSWDDPARPVRSMSLDGGRPCLDLLNTLLPAIPSVQARSEDS
                     RDLLHEYEDLLALAVRTAVLPAAEADALQARGGREPDLAAVALGKTRDLRTLLRRIFL
                     KVLRKEVPSARDLASFEALRSTARSLEVLVWEDGRFRLRPRYLSEVFDAPSLAFVRDA
                     DEFLRSPDLAKVRVVRINTPEARFALS"
     gene            complement(30591..>32785)
                     /gene="gyrA"
                     /locus_tag="P5313_02840"
     CDS             complement(30591..>32785)
                     /gene="gyrA"
                     /locus_tag="P5313_02840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HRY79334.1"
                     /translation="SLRYPLVHGQGNFGSIDGDPPAASRYTEAKLSRVGEEMLGDLGK
                     ETVDFVPNYDESLKEPSVLPSALPNLLVNGSSGIAVGMATNMPPHNLLEAGEAVAAFI
                     DDPEISVDGLMRHVQGPDFPTGGIIFGRRGIREAYVTGKGKIVVRGKFQIETQKGGRE
                     QIIFSEIPYAVNKATLVARIADLIRDKVIEGISDLRDESDREGIRVVIELKKGAVPKL
                     ILNQLFAHTALQSTFGVINLALVKGQPKLLGLKDIVSCFVAHRVEVVTRRSRFDLRKA
                     EEKAHLLRGMMIALRNIDEVVRIIKAAKDVDAAKKGLMERFDLDEVQSQAIVDMRLAK
                     LTSLETEKLEAELRETEATISYLKDLLSSEAKIRAVVKDETLALAKKYGDPRRTEIVP
                     DEIEAINIEDLIKKEEMVILLSNKGFIKRVSTAAYRSQGRGGKGSNSTALLEDDFLEQ
                     IFIANTHDYLLFVSSAGKAYWLKVHEIPEASRQARGAHIKSLLAISQNEEITTVVDLR
                     DFSETEFLFMGTLRGVVKKVATRDFANAKTRGIIAINLDEGDRLVSACRTGGENEVVL
                     ITRRGQALRITEKDVRVMGRASRGVCGIRLEDADELCAMLPVDPEERMLILSEYGYGK
                     RVRFDEYNSHGRATRGQRIYDPDDKTGELVGAIAVRTEDEVVVITSQGKTLKLPADSV
                     STQGRQARGVRIVNIDKPDFVIGLDRIVREDEEGPQEPEPKSGQDGQD"
BASE COUNT         5427 a        11020 c        10832 g         5506 t
ORIGIN      
        1 agaccgtcgc gggcaggagg gcgatgatga ccgagagcat caggcggcgg gtgcttactc
       61 cgctgtagat gtgggggctg gaggacagga gcagggaatt agcgggcacg ttgggcctcc
      121 ttctgcttct tggcgcggag gagctgcttg ccgatgcgga agcgctggac cagctggatc
      181 cgggaggggc agacgaagct gcaggtcccg cactcgatgc agtccaggac cccgatggct
      241 tccgcggcat ccaggtcccc ggcgttcagg gcctcgttga ggagggccgg ggagaggcgg
      301 caggagcagg cgcgcatgca gcgcccgcag cggatgcagg gctgttcctg gaagtaccgg
      361 gcctccgcct tgtccatcaa aaggacgccg gaggtgttct tctggatcgg gaagacgtag
      421 gagggcacgg ccggacccat catgggaccg ccggccacca cccgggccag gttctcgtca
      481 tccacctgga ccgtgtcggg caccaggtcc gagaccaggg tcccgatcgg gaccaccagg
      541 ttcttggggg tcttgcaggc cccgccggtg atggtgaagc ccctctcgat caggggctga
      601 cccttgttga aggcgtccgc cacggcggcc agggtgccga cgttctgcac cacgcagccc
      661 acgtccatgg gaagcccacc ggaggggact tccttgcccg tgatggcggt gatcagcatc
      721 ttctcgccgc cctgggggta cttggtctcc aggacgatca cggagatctc ctggccctcc
      781 cggcggcgtt cgtcgatggt cttccggagg agggaaacca ggtgctcctt gttggcctcc
      841 aggccgatga cgcccttggc gacgcccaat gtcttcaaga ggatggccat gccgtccacc
      901 acgtccccgg atcgttcctc caggaggcgc tcgtcgatgg tcaggtaggg ctcgcactcc
      961 gcggcgttgg cgatgaccac gtcgatgggc ttgttcgggg gcggggccag cttgacgtgg
     1021 gccgggaagc ccgcgccgcc catgcccacg attcccgcct cgcggatccg ggccagggcc
     1081 tggtccttgg tgcaggagaa ggcgtccaga accggcagga agtccacggc gtcggacccg
     1141 tccggttcga tgacgatggc cggaccctcc gcgttgttgg gctggaggcg gggctcgatc
     1201 ttcttcacga ctccggagat cggggcatgg acggggaccg tcatgcgccc ctcggcgtcg
     1261 gctatcttct gcccgcgctt gaccgtgtcc ccgaccttga ccaggggctg gatggggggc
     1321 ccgaaatgct ggttgaccgg gaccacgacc tgtttgaccg agcgcacata ttcgatcgga
     1381 agttcgctgg ttcgatcctt ccgctccggc ggatgcgcgc cgcctcggaa tgtctttttc
     1441 tccatatcga aacgctgctc cttatggggt gtcggcctgt ccggtcgacc taccggagat
     1501 cggatggcgc cccggacgtc cgattcccac gctctcgcgg tttccggcct ccagcaagag
     1561 cgggggcggg aggattgcac gatagtcgtg aacggaaggg tcctcgtcct cgcgcgcggg
     1621 gtatgtacag ggttcgggcc gggcgcggcg gagacggggg gcgacgggct atcctatacc
     1681 gtaatacatc accggtcaag attcgcggat ggagaattta tataaaacac tatattagga
     1741 actcaccaca gagtacacgg agctcacgga ggtgaaaaaa ggggagaaga gggatagaac
     1801 cgggtcgccc ggaaacgctc aatattcatc gcttattccc gtaattcatt cttagccttt
     1861 tctccgtgtc ctccgtgcgc tccgtggtga aatcctgaca acgctccttt tcagaaccac
     1921 ttcttcttct tgaagtacgc caccatgccc agggcaatgg cgagcatgac tccccaggtg
     1981 gccggatagg cccagggctg ctccagttcc ggcatatact ggaagttcat cccgtaaacc
     2041 cctacgatga aggtcagggg aatgaatatg gtggagatga tggtcaggac cttcatgacc
     2101 tcgttcatcc ggttggagac cgaggacagg taaacttcct ggatgctcgc ggcggcttcc
     2161 cggtagacct ccagggagtc caggacctgg accgtgttct cgtagaggtc ccggaggaag
     2221 gggtccaggc cgtcctccag gatgtcggat tgggaatgaa ggagggcccc gaccgcctcc
     2281 cgtacgggcc agacggtccg gcgcatccgg ttcaggtccg ccttgatccc ctggagttcc
     2341 accatgaagc ggtttcccga ggaccgcgcg gcggcgattt cctctcgggt ttccagctgg
     2401 tccccgaggg attccagcac cgagaagtaa ttgtccgtga tcacgtccag cagggcgtag
     2461 gccaggtacg agggtcccag ccgccggagg cggccgaccc cggtccgcag gcgctcccgg
     2521 acggggccga agcagtcccc gggaacctcc tggaaggtca ggaccccgaa gggggtgatc
     2581 acgatgctga cctgctcgta ttcgaccgtg cagtcgggct tccgggtgat catcttggtg
     2641 atcaggagga agtaggtccc gaaatcctcc gccttcgggc ggtgctcggt gttcaggatg
     2701 tcctcgacgg tcagtgcctc cagccggaag tacccgcaca gacgtttcac caggtcctgg
     2761 tccgccagtc cgttcacgtt gacccagttg accatgccct cctcctgcat ggccaggatc
     2821 tcctccaggg actcgggggt gatgaaggag acctgttcgg cgttgtactg gatcagggac
     2881 agcgtcgcct ccacgggggt ccggtctccg atgtagatgg gcgtgcccgg gacgctgccc
     2941 gcggtgccgc tggaaacgct cttgaagatg ggttcgatgg gccgggggcg cgcacgatta
     3001 tgggcatcct tcatggcttc aagagtagcc ccgccggacc ggactggcaa tcccgtttct
     3061 cctgtccaat actggagggg aattggatca atcagaaatc tcttttctgt tttaggaact
     3121 attactatat tattcgaggt tttacgcccg accgagcccc ggctccctcg atgcaaaacc
     3181 atcctggagg atttcatgag cgtccgtgcg cgttggagtt gggcagcatt gggttccctg
     3241 gccttcctgg ccgcgtcctg cggcaaaccc gacggggtgg ccggtgccac cgcctgggaa
     3301 ggtcaacaga cgaccgaaac ggtggcggtg gaatccctca aggtagtccg gggcagcctg
     3361 gcggacgaga tccgcgcatc cggcaccgtc gcggggatcc gggaggcctt cgtggtctcc
     3421 gagacccagg gcctcgtgaa gagcgtgacc ttcagcctgg gggaccgggt cgaggagggc
     3481 cgggagctcc tgcgggtgga cgaccgcata gccgccttga acctggaccg gtcccgggag
     3541 cagtacgagg ccgcccggct ggagctggac gccacggaaa agctggccgc cgcgggccgg
     3601 gccgccccgg cggacctgac ccgagctcgg ggcaacgcca gcggggccaa ggcccagtac
     3661 gagacggccc tcaagtcctt caacgacacc cgcctgcggg cccccgtctc cggggtgatc
     3721 gccgccaagg acgaggtggc caccgtcggc gcaacgatag ccccgggggc ccgggtcgcc
     3781 cggatcatcg acacatcctc gttccggatc acggttggcg tgggcgagcg ggaggtgggt
     3841 ttgatcgagc agggagcggc cgtgaaggtc tacgtgcccg cggccctggg ggaggacttc
     3901 gtagagggga ccgtggcggc cgtggccgcg ggagctgacc ccgcgaccgg aagctttccc
     3961 gtggtcgtcg ccttccggaa cgggtttgcc ggccgggtga agtccggcat ggccgcctcg
     4021 gtctccatag cggctcgcaa atccgaaccc ctggtcgtga tccccagcgc cgccctggtg
     4081 cgccgcggcc cggacttcgc ggttttcgtg gaccggggag gcaccgcgga ggtgcgcccc
     4141 gtcaccctgg gccgcaggtc cggggttcgg gccgagatcc tttcgggtct ggcggaaggc
     4201 gaaaagctca tcatcagcgc cctgacccgg ctccggcccg gggttcccgt ggtatccacg
     4261 gagcggggcg acacggcgga ctgggaatag gagccggaca tgaacctgcc caaattctcg
     4321 accgagaaca gcgtcctgct caccatcgtc ctggtcacgg tcctggtcct ggggaccttc
     4381 agcatagccc gtatgcccca ggaacagttc tcggaggtgc cgtttttctg ggtgaacata
     4441 gcggtcccct atcccggggt ttccgccggg gacatcgaac gctccgtggt cgtcaagatc
     4501 gagcgggaga tgcagggctt ggacaacctc aagcgtatcc agagcgtcgc ctccgacggc
     4561 ctggcgatcg tgcgggtcga gttcgacgac gggatctcca acgacgagtt cgaccgcctc
     4621 ttccaggacg tgcgcacccg gttcggccgg gtggaactgc cggatgggac cctgcaggcc
     4681 ctggtggacg atttctcgtc caacgacttc ctgccggtca tcgaggtcgc cgtctcgggc
     4741 ccggccgcct acgaggagtt gaaccgtatc gcccggaccc tccgggaccg gatcctggag
     4801 gtcaaggacg tctccaccgt ggacctggtc ggggaccggg accggcggat cgtcgtggac
     4861 gcggaccggg aacggttgga agcggtcgga atatccctgt ccgaggtcct acgcgcggtc
     4921 cagcagaaga acgtgaccgt gccgggcggc accctggaga cggtgtcccg ggaatacatc
     4981 ctgcggaccg tgggttccct gcaggggatc ggggatttcg acaaggtcat cgtccgccgg
     5041 gtgggaccct ccggggcggt ggtccacgtg ggggacgtgg cccgggtcta cgaggccttc
     5101 gacgaaagcg gggacacagc ccggatcaac ggggaacgcg ccgtgatcct ccgagtgggc
     5161 aaggttcccc ggggctccgc catcggggtc atcgacgggg tcaagaagat agtcgaggaa
     5221 acctggccgg gcctgccgga gggcgtccgg atcgacctgg tcaacgactc caccgtgcag
     5281 atccgggaca gcgtggacgt cctggtgtcc aacgcggtcc aggggctcat cctcctgtcc
     5341 gtgatcctcc ttctgttcct gggagtccgg aacgccctga tgacggccct gggcatccct
     5401 gtgaccttcg cgatcacctt cgtgatcctg gaggccatgg gggaaaccct gaactccaac
     5461 accctcttcg gcctggtcct ggtcctgggc atgatcgtgg accacgccat cgtcatcacc
     5521 gagaacacct accgcctgcg gtatatgggg tacgacaaga aacgggcggc catagaaggc
     5581 accgcccagg tggcctggcc cgtcgtctcc tcatccctga ccaccgtggc ggccttcctc
     5641 cccctcatgc tcctgcccgg cacgatcggg aagttcctgc gggtcattcc cctgacggtg
     5701 atcatcgccc tcctggcctc caccctggag gccatcctct tcatcccctc ccatgccgcc
     5761 gagtggccgg ggggagacag gctgagctcc aagccggact tcttccagcg catccgcccg
     5821 ggcttcgagc gcttcgtgaa gggactgtac ggccgtcgga ggctcgtggt cctcggattc
     5881 gcggtcgtga ccgtcgccat cttctcgacg attcccttca tccgccagga cctcttcagc
     5941 gccgaggact tcaccctgtt ctacatcgac ctggacatgc ccgcgggcac gaaccgggag
     6001 gcgaccgagc ggaccatccg ggccttcgag gagcgcatcc tgcccctgaa ggggaacggc
     6061 gaggttgcca acgtcatctc ttccgtggga ttccgatcgg acgggagcgg ggggacctcc
     6121 gcgggcaacg tgggtcagat cgtggtggac ctgacggagg ttcgggaggg acgaaagcgt
     6181 tccgtgaccg cggtgatgga cgagatcaag gggatgacct cggatatacc cggaccggag
     6241 aacgtgctgt accggaaggc ccaatccgga cctcccgtct cgccccccgt gggattccgt
     6301 atccggggcg acgactacga cggacttatc gagaccgcgg ccctgatgcg ggagcgcctc
     6361 gccggatacc ccgaactctt caacatcgag gacactctcc aatccggcac cccggaactt
     6421 cgggtccgca tcaatgagga gcgggccgcg tcctacggtt tatccgtggc cgccgtcgga
     6481 tccttcattc ggggatccta cgacgggctc cccgcgggca cggtcttctt ccgcaacgag
     6541 gaactggacg tgatcgtgcg gtacgagggc accgggggag cctcctcggt ggagcgcctc
     6601 cgggagctca agattcccgc ggcggacggc cgcctcgtcc ccttctccgc cgtggcggag
     6661 ctcgtggagg gggccgccct ggcctccatc aagcgcgtgg acggcaagcg ggaagtggag
     6721 gtcgcttccc aggcctacga caagaaggga atccagggga tcaacgcgga catccgcgcc
     6781 tggttccgga ccgaggtcgc ctcccgccat cccgaccaga ctctgatcgt gggaggcgag
     6841 ttcgcggagt tcgcggacct cctggtccag atcctgcggg tcttcctgat cgggctcttc
     6901 ctgatctacg cggtcctggg agcccagttc aagagctaca cccagccctt cctgatcctc
     6961 ctgtccgttc ccatcgcctt ttcgggggtg atcctctacc tggtattctc cgggactccc
     7021 ttctccacga cggtgctgta cgcgggggtg gccctggcgg ggatctccgt caacgactcc
     7081 atcgtcctca tcgacttcat caacgaggac cggaggaacg gaaagcccgt ggcggaggcc
     7141 gtggtggacg cggccgtcac ccgccttcgg cccatcttcc tgaccagcgt caccaccatc
     7201 gcgggcttgc tgcccacggc cctgggcctg ggcggatact ccgtggtctg gagtcccatg
     7261 gcctccacga tcatattcgg cctcatcttc tccacgacca ccgcggtcac cgtggtgccg
     7321 gccctgtacg gcctcttgtt cgaccgcagg ccccgggtcg cgaagggagc cgcggtatga
     7381 acacggtacg aaggaaaatc gtcatgaacc ctgcgttcct gcgggcggcc gcggccgcct
     7441 tcgccgcgct ggcggcgatc ctgccggccg ccgcccaggc cgccggaccc ggtccgaccg
     7501 gatcccccct caccctgtcc caggtcgccg tccgggccct ggagaacgat ccggcggtgg
     7561 ccgcctcccg tgccgccgcc cagacggccc gggttcagta cgagctcaag ctggacgagg
     7621 cccggccccg cttcatcctg gggctgaccc ccttttccct ggaccagcgg cggatcttcg
     7681 atttcacggg gttcgactac gccgacgccc gcaccctgag cgccggcggc ggccttaccc
     7741 tgaatcaggc cctgcccacg gcgggcactc tgagcgccgg ggccaaggcg gccttcaagg
     7801 cgatcgacac ggacggaacc ctggaatacg agcttacccc gtccctgtcc gcgaacctcc
     7861 gccagcccct gggggcgggc ggccagttcc tgcccgtagc cgcggcggcg ggggccaagc
     7921 gctccgccga actggcgtcg gaccaggcgg ccctggacga cctgggccgc cgcaaccagg
     7981 cggtccgggg ggccgtggac ctggcgggcc gggtcctggt actccgggcg gtgctggaca
     8041 cccagacggc cggtctggag accgcgcgca ggcgggcgga gagcgccctg ctccgcaggc
     8101 ggggggggac cgccacggag gacgcggccc tggagctgga gttggccgcg gagaccctcc
     8161 gggccgcccg ggaggagacc cggctttccc ttcgggacgc cgaacggagg ctcgcgggcc
     8221 tcctgggcct ggccgaggct cccgcccttt ccggccggct tccggctttg gcccccgccc
     8281 cggctcccga accccgtacc gccccggagg cggcccgggc cgccctggcc ttggagaggg
     8341 ccgccgcgga cgccgcggcc cgggccgtat cggacgccgc gtccttcggg gccagcctct
     8401 ccctggaacc caggtatccg gacacgcggg cggacccgga cgacctggga tccgtcctgt
     8461 ccgactactt cggcggcggg accggcgcgg ggatgaacct caacctcacc ctgaccctgg
     8521 acgtgcccct gagcgtccgg gaagcccggg ccctgcggtc ccggtcggac gccctcgcag
     8581 cccgggccgc cgccgcgaac ctccgggcag ccgagcagac ggcccgggag cgcgcccggg
     8641 ccctggagga gcgacgggcc ttcctcgcgg accgggtgga gatccagaaa cggatcgcgg
     8701 acctctcccg gcgcaagtgg gagcgcctgc gggacctggc cgcggcgggc acggtccccg
     8761 cggacgaggc cgatgcggcc cggacggact gggaccgtgc cgtctcggac gtgctccgga
     8821 ccgaggtgga tctggtcctg gcggaactgg atattcggat actgcgggga gaggacctgg
     8881 ccgtcgtcct ggccggggcg aagtaggaag gtttcgaggc gcccgggtat cgggcaccgg
     8941 cgcggcggcc cgggctgacg gaccgccgcg ccgggcaggc cctcagcccg cggtgatgta
     9001 ctgctggagc tggtcgatca ccaattcctg ctccgcgatc ttctccttca ccaggtcccc
     9061 gatggagacg atgccgatca cgttcttctc cccgtccagg accggcatgt gccggaaccg
     9121 ggcatgggtc atacgggcca ggcactcgtc cacggactgg tcgggcgtgg cgaagtggac
     9181 cttctcgctc atgatctccc gcaccagggt gtccttggag agttttccct tcagaacgac
     9241 cttccgcgcg tagtcccgtt ccgaaaggat tccgaccagg cgcttgccgt ccatgacgag
     9301 tacggcgccg atgttgtgct tggccatggc ttccagggcc gagtacaccg tatcgccggg
     9361 cgccacggtc acggggaggg tcatcttgtt ccggagtacg tgcttgataa gggtcatggc
     9421 gggctccttt ttaccgtatt gccgcggagc cgacagcccg aaagaacctg ctcgggggcc
     9481 gccgtcatcc ttcacgaagg gaaccgaccg gccgtgcggt gcggtcccat gtcctacgga
     9541 tcgggcgttg acgatgcgga gccgaggaag aactcgatcc accttcaact ctaggcagga
     9601 tttatcctgc tgtcaaatat tttatataaa tatttattta atgaataatg aattatctat
     9661 accaagcccc cttcgtgatc ccgtccgtgc ctaccctaca cagccccgcc cccggcggtg
     9721 tatcctttcg gcatggcaac cgtcgacgac aagaagatca tctattccat gtaccgcgtc
     9781 tccaagcgac acggcaccaa gcaggtcctc aaggacatca gtctctccta cttctacggc
     9841 gccaagatcg gcgtcatcgg cctcaacggc tcgggcaaat cgagcctgct ccggatcctg
     9901 gcgggggtcg acaccgaatt cgccggggag acctccgtct ccccgggcta caccatcggt
     9961 ttcctggagc aggagcccaa gctccagacc ggcaggaccg tcaaggagat cgtcagcgag
    10021 ggggtccagg agatcgtgga cctcctgaaa gaattcgacg aggtgaacga ggctttcggg
    10081 gatcccgacg cggacttcga caagctcgcg acccggcagg ccgccctcca ggaaaaactg
    10141 gacgccgcgg acgcctggga cctggactcc cgcctggagt tcgccatgga agccctgcgc
    10201 tgtcctccgg gagaccaggt cgtggacgtc ctgtccgggg gagagcgccg ccgggtagcc
    10261 ctctgccgcc tcctcctgcg aaagccggac atcctccttc tggacgagcc cacgaaccac
    10321 ctggacgcgg agaccgtggc ttggctggag cgccacctcc gggagtacgc gggcaccgtc
    10381 atcgcagtga cccatgaccg gtacttcctg gacaacgtgg ccggttggat cctggagctg
    10441 gaccgggggg agggcatccc ctggaagggc aactactcga gctggctgga ccagaagcgc
    10501 cagcggctgg agcaggagga gaagggcgag agcgagcggg cccggaccct ggcccgggaa
    10561 ctcgagtgga tcggcatgag ccccaaggga cggcacgcca agagcaaggc ccgcatctcc
    10621 cagtacgaga agctcctggc cgaggacggc cgggagaaga tcaaggaaac caggatcttc
    10681 ctgcccgcgg gtccccggct gggatccgtg gtgatcgagg ccaaggggct ttcgaagtcc
    10741 tacggggaca aggtcctctt ccagggactc gagttcgagg tacccccggg ggccgtggtg
    10801 ggcatcgtgg ggcccaacgg ggcgggtaaa accaccctgc tccggctgat cacgggggag
    10861 gagaaacccg actccgggac cctgcggctg ggggagaccg taaagctggc ctatgcggat
    10921 cagatgcggg ggaacctgga tccggacaag tccgtctggg aacagctctc cgggggcctg
    10981 gacatcgtca agctgggggg aacccgggag atcaactccc gggccttctg ctcctggttc
    11041 aacttctccg gggcggacca gcagaggaag gtcggggtcc tgtccggggg cgagcggaat
    11101 cgcctgaacc tggccctgat ggtccagacc gccgcgaacg tcctcctgct ggacgagccg
    11161 acgaacgacc tggacgtgaa caccctgcgc gccctggagg aggctctgga ttccttcgcg
    11221 ggctgcgccc tggtggtcag ccacgaccgg tggttcctgg accgggtggc cacccacatc
    11281 ctggccttcg aggacgggga ggtggtctgg tacgacggca actggaccga gtacgccgag
    11341 taccgccgtg agaagctcgg cgcctccgcg gaccggccac atagatacgt ttatcgaaag
    11401 ttggagagat gatcggccgg tcggcggttt gttggcggac ggttcgcgtc tcgcgcgaac
    11461 cgaaccgccg ggcccgcggc ccggcgcggc cgcataggta tgtataccga aagttggaga
    11521 gatagcccat ggcgcggtac gtactgctcg cccttcccta cgccctgcag ctcctgtgca
    11581 tcgtgcacgc catgcggacg ggccgggcat actactggat ctggatcgtc ctcttcctgc
    11641 cctacgtcgg aggactggcg tacctgatcg tcgagatcct tccgggcctg cggctgcggc
    11701 ggcaccttgt cgtcgcgcag accgccgtgg atcgggtctt cgtcccggca aagaagcttc
    11761 gggacctgcg gaacaaggcg agcttctccc cgaccgtgga gaaccggatc gaatacgcgg
    11821 acgccctggc cgaggcgggg gcccgggagg aagccctggc ggagtatgaa gcctgccttg
    11881 gggggctggc ccggaacaac ccggccatcc tctacaaggc ggccgcggcc gccttcgagg
    11941 cgggggactt cgagcgcgcc tcgggttacc tcggccgact gccccggaag cccgacggaa
    12001 ccttcgagca gaagccctgg aacctcctgc atctccgcat cctggagcgc accgccaccc
    12061 cggaagccgc ggccgccggg ttcgagcgtt tctgcaccct ggtgaacgac cgggagattg
    12121 actgcctgta cctggagttc ctggagcgga ccggcgcgta cgacaagctg gagaccgtcc
    12181 tggaacgaat ccgcgccgag gaggaatccc tccggggcat gaacgtccgc tacgacaggg
    12241 cgctctaccg ccgggcctat gccgtgggca aacgggcccg ccgcccggaa tagcccgcgc
    12301 cggcggttcg cggccctcgt tcgggataaa atccttgccc gccggttcgg atgcgggata
    12361 ctatacgaag gaacgaaatc cgaaaacgga gcgaactacg agccgaaaat cctggtttcc
    12421 cgcgatctcc atccggagtg ccctccttct gatcctggtg gccgccctgg ccccggcctt
    12481 ccacatcatc gccgtgaccg gagccgagca cgcccggcac atggaggagc aggcggccgc
    12541 ggaagccctg cggcaagccg cggccttcgg agacattcaa acccggatta ccgagtccac
    12601 ccgacagatg ctggccgtcc tggcggccct tccgtcgttc cggtcccgga acgacgacat
    12661 cgcggagcgg accctgcaag ccgtccacag cggcaacccc gaatacctga acctcagcct
    12721 gaccgacggt gccggccggg tcacggcctc ctccctcctg gcccggggta cggacctgtc
    12781 gatccgcccc cacgtacacc gggccctcac gtccggccgc ttcagtaccg gggagtatat
    12841 cctcggcctg gtccagtcgg agccctcctt ttcctactcc ttccccatcc cggacgagtc
    12901 cggccgaccc tacgcgacga tcaacgcctt atacaagctc tccgcctatg ccgtcctctt
    12961 cgagaccttc aaactcgatc cggactcctt cctgggcatc gtggaccgga acgggatacg
    13021 cctttttttc tatcctcccc gggacgccaa tcccgtcggg aaacccatca aggaggaagt
    13081 ctggcttcgc atccagcggg gggacgacgc ggggaccttc ctggatacca gctccgacgg
    13141 agtcgtccgc ttcttcgggt accggaagct ccggctggaa ggggacccgg cgccctacat
    13201 caccgtggtc tacgcggtac cccgggaaaa gatcctgaga gcaagccgca ccatcacccg
    13261 acgaaacctc ctggccatgg ccggggcggc gatcctggct ttggccctgg ccgggatact
    13321 ctccgaaatc ctcttcggcc ggaggctttc ccggatcggg tcggcggtgg accggatccg
    13381 gggaggggac ctcggagccc gggtccgtct ggagggagat tcgtcggatc tcgggcggat
    13441 ctcggcggcc ctggacggca tggcggaatc ggtccagagg cgggacgcgg aaaaggagga
    13501 ggaagccctc cgcctgagcc gtcttctgga cgagaagagc gtcctgctca aggaaatcca
    13561 ccatcgcgtc aagaacaatc tccagatgac cctgagcctg atccgccttc aggagggcgc
    13621 cagttcggag gacggcgagg cgtccttgaa gggactggaa tcccggatct ccgccatgtc
    13681 catggtgcac gagatgctct acggatccga aaatctgacg gacatcgacc tgggcgagta
    13741 ctgcggccgc ctgctcgatc ttctgaggag cagctatgcc gggaaaaacc ccctccaggt
    13801 ctctttaacc tgcgagaccg tacgttgtcc cctggaatcg gccatcccct tcgggttgat
    13861 cacggccgag ctcgtaacga acgccttcaa gcacgcctcc ggacggggtc ccgccgcggt
    13921 ctccgtggtc ctggcccata aggacggaac ggcctgcctt accgtttccg acaacggtcc
    13981 cggtctgccc ccgggcttcg atcccgcggg gtcccgacgc ctcggcctcc aactcgtcct
    14041 ggccctgacc tcccagctga gaggaaccct cacctgggag aacgacggcg gcgcccggtt
    14101 ccgggtcgac tttcccctgg ataccccgaa aggtcccggc gccccggatc ctgaggcagg
    14161 atagggccga tttcggaaaa atgtatcttt ttacagaaac aagcccttgt cccgcacggc
    14221 cgttttattg gataatcccg caccagtctt cctgtttcgg agagtgccat gtgcggaatc
    14281 ttcacggtcc tggatatcaa gggtaacccg gtcgagctcc ggaagctcgc catcaaccgg
    14341 gtcaagcgca tccgccaccg cggcccggac tggagcgggg tctactcgga caagcgtgcg
    14401 gtgatctgcc acgagcgcct ggccatcgtg gacgtcaccc acggtgccca gcccctggtg
    14461 gacaagggca cgggccgcgt actttccgtg aacggggaga tctacaacca cctggagctc
    14521 cgggcccggt acctcaaaaa agcccacgag ttccagacgg cctcggactg cgagcccatc
    14581 ctctacctct acgacgaact cggtcccgac ttcgtcaagc tcatgaacgg gatcttcgcc
    14641 ttcgtgatct acgatccccg ggacggggac ttcttcatcg cccgggacca catcggcatc
    14701 aaccccctgt actggggtcg ggacggggag ggcaacctct tcgtggcctc ggagatgaag
    14761 gccctctacg acgtctgtcc caaggtggag gtcttcccgc cgggacacta ctacaagggc
    14821 tccgagggca ggctggtgaa gtggtacgag cccgcctggg ccgctcccgg gaggatcccc
    14881 gacggggagg cggacctgga agccctccgg gacgccctgt gctcggcggt caagcgccag
    14941 ctcatgtgcg acgtgcccta cggccttttg atctccggcg gagtggactc ctccatcatc
    15001 gccgccatcg ccgcgaagtt ctccgaaaag cgcgtggaaa gcggggacac cgagcgggcc
    15061 tggtggcccc ggatgcactc cttttccgtg ggcctcccgg gcgccccgga cacgaaatac
    15121 gcccgcaagg tggccgagtt catcggcagc caacaccacg agatcgagtt caccgtccag
    15181 gaagccctgg acgccatccc cgacgtcatc taccacctgg agaccttcga cgtcaccacc
    15241 atccgggcgg gcacgcccat gtacctcatg tcccgggtga tcaagtccat ggggatcaag
    15301 atggtccttt cgggggaggg gtccgacgag atcttcggcg gttacctcta cttccacaag
    15361 gcccccaacc ccgtggagtt ccacgaggag ttggtgcgca agctttccct gctgcacctg
    15421 tacgactgcc tccgggccaa caaggccatg accgcctggg gggtcgaggc ccgggtgccc
    15481 ttcctggacc ccgagttcct ggacacggcc atgaacatcg accccaagca caagatgatc
    15541 cgcaaggacg agggccgcat ggagaagtac atcctgcgca aagccttcga cgggtgggtg
    15601 cccgacgagg tcctgtggag gcagaaggaa cagttctccg acggggtggg gtactcctgg
    15661 atcgactcca tcaaggccca cgcggaaaag gaggtctccg actccgccat ggccaacgcc
    15721 cggcaccgct tcccggtcaa gacgcccccg accaaggagg cgtactggtt ccggtccatc
    15781 ttcgaggagc acttcaagaa cccctcggcg gtggacctgg tccccgacgg gccctccatc
    15841 gcctgctcca cccccaccgc catccgctgg gacgccgagt gggccaagct ggcggatccc
    15901 tcgggccgcg cggtccgggg cgtccacgat aaatccaatt gaggttattg gaaagaatca
    15961 cggaggacac ggagggtccc gcttaagagc gacaggataa caggataaaa gcggattgac
    16021 aggataagat atttgtaaga atggttttat cctgtgatcc tttccaaatc ctgtcatcct
    16081 gtcgtttctt ctcctcgatc tttctccgtg tactccgtgt actccgtggt tgattcccga
    16141 aacacactcg tgtatcgttc atcgaagcgg tgggccgcag agggaggaaa cgatggacag
    16201 gaacgagaaa gaggccttga tcgtacggta cgagacggcc ttcgagccca tacggcacct
    16261 tgtgaaggac ctctccgcca aggaactttc attccagccg gacctgcccg aggcgtggtc
    16321 catcaacgag cacctcgttc acctcctgga agcggacctg atctgctggt accgcatccg
    16381 cgcctccatc gcggagccgg gcgtcaccat cccggtctgg aaccaggagg cctggcggtc
    16441 ccggctgcgg tacggggacc tggacgggat cgggtgcctg gcggaggccg aacgggtccg
    16501 ggccctgatc gggcggacct gccgcggagc cctggacggg gactggtccg agttccacgt
    16561 catccatccc gagagggggc ggatggacct ggcccagctc ctggagctct actgcggcca
    16621 cggggccttc cacctcaagt acgtcgagcg gaacctggcc gcctgcaggg cgcggtaagc
    16681 cccgcggagg gctccgggcc tcccggcgga tcccgagcgc taggtccagg cctgcgccca
    16741 ctcctcgatg ccccgcatgg cgggttccag ggcgaggcct ttttccgtca ggcgatagga
    16801 cacacggatc ggagggcccc ccacgaccgt gcggtggacc aggcctgcgg cctccagttc
    16861 cttgatgcgc agggccagca tgcgggagga gagggagggc accgtccgct ccaggtcgga
    16921 gaaggaacac tccccctcca gaagctggtg caggatgagt ccggtccatt tccttcccag
    16981 gagctcgaag gccgcctgca cccggggaca cagcttctct cgctcttctt gatccatacc
    17041 tagatagtag cattttcctt tactcggctc aatataatta ttaacttatt attcgtaaca
    17101 tgctacgata agagaagcca cattcggcag gaaggagtac ccgatgaacg atttcagcgc
    17161 ggccgccccc cgggagcgga tcgaaaaggc tatggcggcc cttcgggcca acggcatcga
    17221 cgcggtgttc gcccgggacg ccgccgcggc gaaggatacg gtgttggaat tgatccccgc
    17281 cggagcggaa gtgatgacga tgacctccgt gacggtcgac tccatcggcc tcgggaaggc
    17341 cctcaacgag gggggacgct tcgacgccgt caaaccccgg ctctcctcca tggacccgaa
    17401 gacccgcgga cgggagatgc ggaagtacgg ggcggctccg gacgtggcgg tcgggtcggt
    17461 ccacgccctc accgaggagg ggcacctggt gatcgcctcc ctgaccggga gccagctccc
    17521 cgcatacgcc tacgggtccg cgaaggtgat ctgggtcgtg ggggcgaaca agatcgtgag
    17581 ggacctggag gaaggaatcc gccgcgtgga ggactacgta ctgccccggg agagcgcgag
    17641 ggcccgggcg gcgtacggac tcccggacag tttccactcc tatccttcga agatcctcct
    17701 gattcggcgg gaggtgcagc ccggccgggc cgtcgtcgtc gtcgtggggg aaagcctggg
    17761 cttctgattc ccggatctcg tatcgtcccc gccccgggca atcctccgac ggcggccgcc
    17821 tccccgggcg gccatggacc acggcacgga cttccacatc gccgggccgg gaatcgggtt
    17881 ccgttccgtc gccgataccg ggttgccccc ccgccgttcc ggtgacatac tcccgtcggg
    17941 gccttcggga gactcgaagc ggcccggcag gagcgcgcca tggtatcgga aaacatacgc
    18001 tcggcctatt cggccatcct gaacgaggaa ctggtgcctg ccctgggctg cacggagccc
    18061 atcgccgtgg cctacgccgc ggcccgggcc cgggagatcc tgggccgtat gccggagggc
    18121 ctgtgcgtac gctgctccgg gaatatcgtc aagaacgtga agggcgtccg ggtgcccaac
    18181 accggaggcc tcaagggcat cgcggcggcc gccgtggccg gggtcgtagg cggggacgcc
    18241 tcccggcgcc tggaggtcct ggagggggtg cgtccggacc agctcgagga agtacgacga
    18301 ctcctggccg ggggatactg cgaggtaagc ctggcggagg gggtggaaaa cctcttcatc
    18361 gaggccgaag ccttcgacgg ggacgatacg gccgtggtcc gcatcgaggg ctcccacacg
    18421 aacatcaccc tagcctcccg gaacggcgaa tccgtgcggc attccgtcgc cgcgcccgaa
    18481 gccggaagga agccggaatc ccggggcgac gcgaccgcgg gcccccgcgt cgaggggggc
    18541 gccctggcct cgggcgcggg attgctcaac atccgggaca tcctggagta cgccgagacg
    18601 gcggacctgt cggacgtgga acgggtcctg gcggaccaga tcgagatgaa ccgggccatt
    18661 tccgacgagg gactgaacca tgcctggggc gcccaggtcg gcaagaccct gctcaagacc
    18721 cggggcaccg acgtgcgggt ccgggcggcc gcccgccccg ccgcggggag cgacgcgcgg
    18781 atgagcggct gctccctacc ggtggtgatc aattccggca gcggaaacca ggggatgacc
    18841 gtcagcctgc cggtcgtgga atacgcccta gaactcgggg ttccgaggga acggttgatc
    18901 cgggccctgg ccctgtccaa cctggtggcc atccggcaga agatccggat cgggaagctc
    18961 tccgcctact gcggggccgt cagcgccgcc tgcgggagcg gggcggggat cgccttcctc
    19021 cggtgcgggg gctatcgcga gatctgcgat accatcgcga acaccctggg caacgtgggg
    19081 ggcatcgtct gcgacggggc caagccctcc tgcgccgcca agatcgccac ggccgtggag
    19141 gccgccgtcc tggcccagga cctggccttc gagggcaggg ctttcgcgga cggggagggc
    19201 ctcgtctccg aggacgtgga gcggaccatc gagaacatcg gaaccctcgg ccgggagggt
    19261 atgcgggaga cggacaaggt gatcctccgg atcatgacct cggactgata ccggaccttc
    19321 cgcttgcgac aagcggaccg atacgctacc ctttgtccat ggccaagccc tcggattgga
    19381 aggaactggg ttccggcagg atcgcggact gccggatctt cgacgtctcc tccagcgacc
    19441 gggcctgccc ggacggcaag cggggaacct tcttcctggt gaacgccccg gactgggcgg
    19501 gggtcatccc cgtggtcgac actcccgagg gccgcaagtt cgtgatgatc cggcaattcc
    19561 ggcacgggac gggaaggctc tccgtggagt ttccgggggg catcgtggac cagggggagg
    19621 atcccgccca ggccgtggcc cgggaactcc tggaagagac cgggtaccgg gcggatctcg
    19681 tcgttcccct ggggcttctg tcccccaacc ccgccttcat gacgaacacc ttccacgcct
    19741 tcatcgccga gggctgcatt ctggaacgtc cccaggccct ggacgagcat gaggacatcg
    19801 aggtgctcct ggtgcccgaa cacgaggcca tcgacctggt gggcgcggag gactacggcc
    19861 acgccctcat gaccgccacc ctgttcctgt acatgcgcta ccgcggcttc tgcccgtgag
    19921 agggaggcac cgttgaacgt cgagatcacc gaaccgaggg aactgctgga tccggacgga
    19981 cggatcacga aggaaggctg ggcccggaaa ccgttctggc gctacgaccg gtcccggatc
    20041 aaggctccct ggtggcggat caaggagtgg gattactact ccgtcctctc ccgggacggg
    20101 aaattcggga tcaccctgac cctctccgac ctgggctacg cggggctctg cgccgtctgc
    20161 ttcctggaat tcgagcgccg ccatgtatgc caggtcgata ccctctcggt cttgcccatg
    20221 ggtcgcaccg gactgggacc cgacagcgac gccgggcacg tcgccttcgc ggattccaag
    20281 ctggccctgg atttcgagta cctcccgggc aagcggatcc tgaccttcgc ggcccccggg
    20341 atccggaacg cccaggggga cgagggtctc acgggaagcg tgaccctgtc ccagcccgac
    20401 ggcctggaga gcatgaacat cgccacctcc tgggcggaga accggagggc cttctactac
    20461 aaccggaaga tcaactgcat gcccgcctcc gggtggttca cggtcgggga ccgtcgctac
    20521 gagttcgatc ccgcccggga tttcggggcc ctggactggg ggaggggacg ctggacctac
    20581 cgcaaccggt ggtactgggg ctcggcctcc ggccatctgg acggccggcc cttcggctgg
    20641 aacataggct acggattctc ggaccggagc cccgccagcg agaacgccct gatcctggac
    20701 ggacggatcc acaagctggc ggaggtggag ttccggatcg acacctcgga ctacctgaag
    20761 ccctggcgct tcaccagctc cgacggccgc ttcgagatgg acttcgagcc cgcggtggat
    20821 cgggcctcgg agacgaacct gggggtgatc cgctcggtcc agcaccaggt cttcgggagc
    20881 ttcacgggaa ccgcaatctc cgacgacggg accaagatca aggtgaagga cttcccgggc
    20941 ttcgcggagg acgtgctgaa cctctggtaa acgtcacgcg aacttgtagc cgaacctccg
    21001 gagcaaggac ttccgatccg acttgtccgc ctcgccctcg acgcccttgg gggaggaccc
    21061 gtccacgacc ccgacgatcc ccgccccctg gggggtgcgg gccacgagga cctggagagg
    21121 attgccggtg gcgcagaaga cccggcagac ctccgggcag gacttgatcc ggtccagggc
    21181 gtggaggggg aaggtccccg gccccaggac gacgtagaag gtgtggcccg ccccgacggc
    21241 cttggcgcag gccgcggcgg tctcgaccag ggagggatcg ttcccctcgg tccggaccag
    21301 gcagggcccc gaggcctcgt tgaaggccag gccccatttc gccccgggta ccgcggccag
    21361 gatctccccg aggtcctcca cggtcttgat gaagtgggac tgccccacga tgatgttgtt
    21421 cttcccctcc ccgtagtcca gggcgatcgt ttcgatggcc gcgttcattc cggatcctcc
    21481 gttccgggca gggcgagcgc cctgtcctcg gtcttgaacg atgataccgc cttctccaga
    21541 cggacagatt cctccgaaag ggaggcggcc gaggccgcga gctcctcggc ggcggcggcg
    21601 ttctgctgga ccacctcgtc cagggccagc accgccttgg agatctgctc cgttccgatc
    21661 tcctgctccc ggctggccgc cccgatctcc tccaccaggg tagaggtccg gcggatggcg
    21721 ggcaccaggc gccccaggga ttccagggcc cggtccgact gcttctgggt gtccgcggaa
    21781 attccgcgga tctcttccgc ggcctttccg ctccgttccg ccagcttccg aacctccccg
    21841 gccaccaccg caaagccctt gccgctctct ccggcccggg cggcctcgat ggcggcgttc
    21901 agggccagga ggttcgtctg ccgggcgatc tcctcgataa ccccgatccg gtccgcgatg
    21961 gagcgcaggg cctgggcggc tccccggaag gaatcctcgc tctccgcggc ctcccgggcg
    22021 gcctccgagg cgatgccggc ggtttcccgg gcgttctccg cgttgcgccg ggccgtggcg
    22081 gagagctcct ccacggaggc ggaaacctcc tgggcccccg cggcctgccg gctggctccg
    22141 tcggagatcc cgttcgaggc ttcccggaac tgcccgctcc ccagggccac ccgccgggag
    22201 gcctgggcgg cctggcctat cagtcgggac aggtcgtccc gcatggccga gagccctcgg
    22261 acgatccgac ccgcctcgtc cggccgggcc aggaagcccg ggtcgaagtc cccggtcaag
    22321 tccccctcgg agattcggcg gacgcgctcc accgcgtagg aaaggggtgc ggagatgcgc
    22381 cgcccgacca cgacggccag gacgaccatg accgccgcga gcagggcggc caggggcacc
    22441 agggctccca cggcggcccg gagccgggcg tccgcctccg cccgcttctg gccgacgacc
    22501 ttctcgatgt cgtcgatgta attgcccgaa cccacgaccc agttgaagga gggggccagc
    22561 atgctgtagc cccgcttcaa atttgcctcc gtctccccgg gccgggggaa ccagtagtcc
    22621 gtatatcccc cgccggccag ggccgccccg atgatggcct tcacgagctc gaagcccttg
    22681 gcgtccttca ggttgtaccg gttcgtcccc tccgaggcgt tccccaggag gactttgttc
    22741 accccctccg gggtgtccgc ccagaagtag ccgtcctttc cgtaccggag gtcccgcagg
    22801 aggtctcggg ccagcttctc cgcggcctcg gggccgagct cgccggcgtt ccgcatcttc
    22861 ccgatgcgtt cgaccaggga gaccgcggac tccacctggg acttgatgag ccggtcgtag
    22921 tcctcccgga gggtggcttc cagggccgag atctggtcgt tcgaggcgga gacgaagagg
    22981 tacccgaaaa cggcggaaag cccgatcgtc acgaccaggg cggcccccgc ggccagcagg
    23041 atgatcttcg cggtgatcga cttcatccgt ttctccttcg tatgaggcta ttccgaggaa
    23101 aaccgtccga tcggcgcggc gtacagggcg aaccggtccc tgccgtccag ccccaggtag
    23161 gcgtccagct tctcctggtc gtaggctccg atggcgcagg tccccgcccc gacggcctcg
    23221 caggccaggt agacgttctg gcaggcgtgg cccgcgtcca gggcgatgat cttgtgggac
    23281 accgggccgt agcgccattc cgtacggtac ggcacggcgg cccagaccag gacggccgcg
    23341 gggttccagt cctggccgca gagggcctcc ccgagggcat gggagtcgtc cccgaggcgc
    23401 accagggcca gggcgtgctc caccgggagg tagcggtaga gcccgggctc gaccccctcg
    23461 acccgggcgg cgaaggcgaa gaggtcgaag gggtgccggg ccccgccgga ggggtaggta
    23521 cggaaggaga acttgggcct gtggtccttg atccccgcgg cggcccagca taggaaggag
    23581 aactcctccc gggtcaggta tccgtccgtg tacttgcggc ggctcttccg ggtccgaagg
    23641 aggtccagca gggatccgcc ccggggcttg aactccgagg ccggaacgag ggggaggatc
    23701 cgctcgccct ccctcgggcc ttcctcctgg gcgggggcgg gcacgccctt ggactggtcc
    23761 gacttccagg gatcctcgat caggctccag ttggagcgaa gaaaatttcg tccggtttcg
    23821 taggtttcgc tcatgtcgat tctcctcggt cttccagtgt acatcgaagg tccggccgcc
    23881 ggaagaccgg cctgaggggc ggccctcagc ctcccaggtt cgcggccgcc agggccgccc
    23941 cgcttccgat caggaggatc cgggtcccga tgaacaggac gcgttcgggt atccggccgt
    24001 ccaggacctg gccgagcagg aaccccgccg cgacgaaggg gtacagcccc agggcggccc
    24061 ggaaggtttc gcggccgtac aggcctagag ccgcgtaggc cccgaaacgg cagacgttgt
    24121 ccaggaaaaa caccgcgcac agggtacccc ggagggcatc ccgatcccgg aatcgccggg
    24181 ccaggaaggc cgccaggacc gcccccacgc cgaagaggcc gagggacagc cccgaggcgg
    24241 tcagccggag ggcgtccccg gggccccgga ccggggccct ccgtgcccgc tcttccggtt
    24301 ccgtcgtctc caccgagggc gcccgtcccc ggaccaggac gatcagggaa tccgcggcta
    24361 cgaagagccc caggagcacc ttgagcgggc gtccccggat accgtccagg atcagggcgc
    24421 cccccaggat tcctgcaagc acgaaggcga agagcacgag cacgaacttc acgtccacca
    24481 ggcgccgggc cttccaggcc atcgccaggt tcatggggac gttcgtcagg agctccgcgg
    24541 gggccgcgaa gcgaagctcc gtgaagaaga gaaagaatgt gttcgttatg agcgtattcc
    24601 caaaaccagt cagccgcttc acgacgaagg ccagggtcac gacaaggccg aggagaatct
    24661 ggttggccac gggtcagacg gccgggtcgc aggcgctcac cggaaggacc gggcggatcc
    24721 caccgccttg tcccagccgg acagcaggcg gttccgggtt tccccggcca tctcgggacg
    24781 gaattcccgg cggcgcttcc agttccgctc cacttccttt acgtcggtcc agtaccccgt
    24841 cttgagaccc gccaggtagg ccgcacccag ggcggtcacc tcgttcacct ccggcagtat
    24901 caccgggcgg tccaggacgt ccgcctggaa ttgcatcagg aaggagttgg ccgcggcccc
    24961 cccgtccgcc ttgatatagg gcagggcgga accgaagtcg ttctccatgg accgcagaag
    25021 gtcccgggac tggaaggcca cggactccag ggccgcccgg accaggcggg cccggttcgt
    25081 tcctcgggtg atccccacga tcgtgccgcg gacatcgggg tcccagtagg gggcgcccat
    25141 gcccacgaag gccggcacga tgtagacgcc ccccgtgtcc ggcacggacg cggccagggc
    25201 ctcgctctcg gcggcgtcgg agatcaggcc cagctcgtcc cggagccact ggatcaccgc
    25261 ccccgcgatg aagaccgagc cctccaaggc gtaggtgaag cccttcccga ggtcccaggc
    25321 cactgtggtc aggaggttgt gcttggaggc cacgggctgg gagcccgtgt tcatgagggt
    25381 gaagcagccc gtgccgtagg tgttcttggc cgatcccggc tcgaagcagg cgtgcccgaa
    25441 gagggcggcc tgctggtccc ccgcgcagcc ggtcaccggg atctcccgtc cgaagagctc
    25501 ccgcctcgtc ttcccgaact cggcggagct cgggaggacc tcgggcagga tggaggccgg
    25561 gatcttgagc tccgccagga gctcctcgtc ccagcgcccc gtgtggatgt tgaagaggag
    25621 ggtgcggctg gcgttcgtgg cgtccgtggc atgccgtccg gtcaggttgt agatgagcca
    25681 ggagtccacg gtcccgaagg ccagctcccc ccgctccgcc cgggtccgca atccgggaat
    25741 ctcgtcgaac atccagacca gcttggtccc ggaaaaatag ggatccagga ccagacccgt
    25801 cttctcgcgg accgtcgggg ccaggccccg ggcggcgagc ctcttgcaga tctccgcgga
    25861 gcggcggcac tgccagacga cggcgttgta tacgggcttg cccgtggccc gttcccagac
    25921 cagggtggtc tcccgttggt tcgtgatgcc gacggcctcg atctggtccg gcccaacctg
    25981 ggccgccgcg agggctccct tggcggcctc gtactgggtg tcccagatgg ccgccggatc
    26041 gtgctccacc cagccgggct tggggtagat ctggggaaag ggaatctgcc tgaccccgat
    26101 gatgttcccg ccccggttga agacgatggc gcgggaactg gtcgtgccct ggtccagggc
    26161 caggacgtag gtttcgcttc ggctcatgtt cgactccggc gggaaggatt gttgcgggcg
    26221 cggcgcaccc gtcgggcccg aggcggccgg aaacgccgcc gcatccagcg tcagtatacg
    26281 agaatccgtt cggtttgtaa aaacaaagcc gcccccctct tccttctccc ttcattcttt
    26341 ttccttctcc ccttcccttt tttctccgtg tcctccgtgt ctccgtggtt caatcgggaa
    26401 accgatacct cggggccttg ccgcgccgcc gcgatttcgc gatacttgat ttcgcgccgc
    26461 aagcccgtcg ggcccggggc gcaccgccga tttgctccct attgcgggcg gtcaacaaat
    26521 ccacgctaaa aaggaggccc gccgtggctt cgtccctccg tcatccgaac ccgcgataca
    26581 cctcccgcct caagcgtcct cgttcccggt aaaggagtcc tccatgcgcg acaagtccta
    26641 cctgttcact tccgagtccg tcggggaggg acaccccgac aagctttgcg accagatatc
    26701 cgacgccgtc ctggacgcct gcctcgcgga ggatcccgaa agccacgtgg cctgcgagtg
    26761 cttcgccacc accggcatgg tcctgatcgg cggggagatc accacggaaa cctacgtgga
    26821 catccagacc ctggcccggg acgtggtccg ggagatcggc tacacgaacc cggactacgg
    26881 gatcgactac gagtccatgg ccatcctgaa caccatccac tcccagtccc cggacatcga
    26941 ccagggggtg gacggccggg ggctcaagga gtacgagggg cagcagggcg ccggggacca
    27001 ggggatgatg ttcggcttcg cctgccggga gaccccggag ctgatgcccg ccccgatcat
    27061 gtacgcccac cgcatcctgg aacgggcggc gcgcctgcgc aaggccaagg agatcccctg
    27121 gctacgtccg gacgccaaga gccaggtgac cctgcagtac gatggacaca agcctgtccg
    27181 ggcggacgcc gtggtggtga gccaccagca cgaccccgat atccagtacg cgaccatccg
    27241 ggacacggtg atcgaggaga tcgtgaagcc cgtgctcgga cccacgggct tgcttgacag
    27301 taacacgaag tattacgtca acccgacggg acgcttcgtg atcggcgggc ccatgggtga
    27361 cacgggtctg acggggcgca agatcatcgt ggactcctac ggggggatgg gccgtcacgg
    27421 aggcggggcc ttctccggga aggatccctc caaggtcgac cggtccgcgg cctacatggc
    27481 ccggtacgtg gccaagaacg tcgtggccgc aggcctcgcg gagcgctgcg aggtccagct
    27541 ggcctacgcc atcggggtgc ccttcccggt ctccgtgatg gtggacacct tcgacaccgg
    27601 aaaggccgcg gagcacgtca tctccgacgc gatccgcagg gtcttcgacc tgagccccgc
    27661 ggggatcatc cggaccctgg acctgaagaa gcccatatat cagcgcaccg ccgcctacgg
    27721 ccacttcggc cgagacggtt tcgcctggga gcggaccgac aaggccgagg agctcaagaa
    27781 ggcgctgtag ccggattcgg aggggcggag ggatggccga gatctcctat accctggctc
    27841 gctttctcga ggcggtccgg gattacccgg ccgagcgggt ccaggcccgc ctgctgtccg
    27901 cctcggaccg ggacctggcc gccgtcctgg cggccctgaa acccccggac cgggaagccg
    27961 tgctggcccg gatcggtccc gccaagcgaa cccgggtgga ggagggcgtg gagcggatgc
    28021 ggttcgttcg cctggccccc gggcaggcgg accggatcgc cgcccacttg accgcccaca
    28081 tccaggggga cgtccccctc ggccccgctt cctcccattt ccgaccgcac aagtccgagg
    28141 gagagcggga ttgaatccgg agcccgaagg cgttcccgac acgtccgccg aagcacgccc
    28201 tgcccccgcc ccccgccgcg ccctgcgccg gggggacctt cccgacaact tcacccgttc
    28261 cctgtatggg ctgtccccct accggggctg cgcccacggc tgccgctact gcgacggccg
    28321 ggcggagaag tactacgtgg aaggcgactt cgagcgggac atcgcggtcc ggggctggct
    28381 gccggatcgc ttggccgagg aactcccgag gctccgggaa cccggcatgg tcgccatcgg
    28441 gtcgggcacc acggacccct accagcccgt cgaggcggag gcgcgcatcg tcgggcgctg
    28501 cgcggagctc ctggcggacg cggggatgcc cgtcacggtc atgaccaagt cggacctcgt
    28561 cctccgggac ctggacgcct ggcgccgggt gaacgagcgc tccctcttcg tcctcctgat
    28621 ctcgatcacc accctccggg aggatgtccg ggccgcctta gagcccgggg cctccccggt
    28681 caaggaacgt ctcgaggcga tgcggacctt caagaaagcc ggttgcgccg tcggagccct
    28741 ggccatgccc ttcctgcccg ggatcacgga cgacgaggcg gggatccgcg ttctctacac
    28801 ggaactggcc gccatcggcg tggacttcgt catgcccggg ggcctcaccc tgcggccggg
    28861 tcggcaaaag gacctctacc taaagaccct cggggaatat cggccggagc tcctcggctc
    28921 cgtccgcgac ctgtaccggg aggaccggcc ctcggggcgc cccatccggg cggcggagga
    28981 aacccttcac cggaccatcg ccacggtgcg aaaggacgtc ccccttccct tcctcctgcc
    29041 ccaccgggtc ttctcccggg ccctcccggc ctacgacacc ttccgggtac tcctgcggga
    29101 catggcggag ctctacgcgg accggggcgt cgacacccgg cccctccgga aatcggcgga
    29161 ccgctacgac cagtggctga tcggcctgcg ccgcgtccat cgccggaagc gctccctgcc
    29221 cgattcctgg ctggccgacc ggttcgagga cgccctggat accggggaac tggacggggt
    29281 cctcgacaac cggcgcctgt ccggcttcgc acggtccgtg ctccgggacg gggcgaccct
    29341 ggattatcga accctgcggc ttgttcccaa taccctgaaa tcggattaag taaaagatag
    29401 gaaccacaga gacacggagg cacagagaga aaaggagaga gtgggaaagg agaaaccagt
    29461 tagccaagac attgaatacc tccgatcctt catccttcat ccctcatcct ttcttccttc
    29521 ctccctctta cctctgtgcc ctctgtgcct ctgtggcgaa ttatggatat aacgcttcac
    29581 cgtccgaagc ggccgcgctc tggttatttc ccccgaatag cgatagggtt acggtatccg
    29641 atccgacccg aggtgcgcct atgattcgtt cctgggatga tccggcccgg cccgtgcgga
    29701 gcatgagcct ggacggaggc cgcccctgcc tggacctgct caacaccttg cttcccgcga
    29761 ttccctccgt ccaggcccgc agcgaggact cccgggacct tttgcacgaa tacgaagatc
    29821 tcctggccct ggccgtacgg accgccgtcc ttcccgccgc ggaggcggac gcccttcagg
    29881 cccggggagg ccgggagccg gatcttgccg cagtggccct gggaaagacc cgagacttac
    29941 gcactcttct ccgcaggatc ttcttgaagg tcctccggaa ggaagtccct tccgcccgag
    30001 acctggcctc cttcgaagcc ttgcgatcga cggcccgttc cctggaagtg ctcgtatggg
    30061 aggacggccg attccggctt cggccccggt atttgtcgga agtattcgac gctccctcgc
    30121 tggccttcgt ccgggacgcg gacgagttcc tgcgttcccc ggacctggcc aaggtccgag
    30181 tggtccggat caacaccccg gaggcgcgat tcgcgttgag ttaggcatcc gcaggactca
    30241 accacggaga gcacggagaa gaggaaagga tgagggatgt cctttgtttc acgtgaaaca
    30301 tccaggatcc ttgcgaggta ccctttcaag tttctttccc agaacctgaa tacaccctgc
    30361 ctcttttagt tctcctcgtc ctctattcct ccgtgaactc cgtgttctcc gtggttccct
    30421 caaaaaaaca gccgcccgga gggggcggcc cgattgtttc acgtgaaaca aggtacgtct
    30481 caaaaacccg gacaggataa acagaatcgg aaccggatga acaggatcaa aaattgggat
    30541 ggaactgcta cgatcccact tcctcaattc cttatcctgt tcatccttcc ttaatcctgt
    30601 ccatcctgtc cgctcttcgg ctccggctcc tgtggtcctt cctcgtcctc ccggacgatc
    30661 cggtccagtc cgatcacgaa gtcgggcttg tcgatgttca cgatccggac cccccgcgct
    30721 tgccggccct gggtggacac ggaatccgcc gggagcttca gggtctttcc ttgggaggtg
    30781 atcaccacaa cctcgtcctc cgtgcgcact gcgatggcgc ccacgagctc gccggtcttg
    30841 tcgtcgggat cgtagatccg ctggccccgc gtggcccggc cgtgggagtt gtactcgtcg
    30901 aagcggaccc gcttgccgta gccgtactcc gagaggatga gcatccgctc ctcgggatcc
    30961 acgggcagca tggcgcagag ctcgtcggca tcctccagcc gaatgccgca gaccccccgg
    31021 gatgcccggc ccatgacccg cacgtccttt tccgttatcc ggagggcctg gccgcgccgg
    31081 gtgatcagga ccacctcgtt ctccccgccg gtccggcagg cgctcacgag cctgtccccc
    31141 tcgtccaggt tgatggcgat gatcccccgg gtcttggcgt tggcgaagtc ccgggtggcc
    31201 accttcttca ccacaccccg cagggtcccc atgaagagga actcggtctc cgagaaatcc
    31261 cggaggtcca ccacggtggt gatctcctcg ttctgggaga tggccagcag gctcttgatg
    31321 tgggcgcccc gggcttgccg ggatgcctcg gggatctcgt ggacctttag ccagtaggcc
    31381 ttgcccgcgg aggagacgaa gaggaggtag tcgtgggtgt tggcgatgaa gatctgctcc
    31441 aggaagtcgt cctccaggag ggccgtggag ttggagccct tccctccccg cccctggctg
    31501 cggtaggccg cggtggacac ccgcttgatg aagcccttgt tggaaaggag gatgaccatc
    31561 tcctccttct tgatcaggtc ctcgatgttg atggcctcga tctcgtccgg gacgatctcc
    31621 gtgcgccggg gatctccgta cttcttggcc agggccaggg tctcgtcctt gaccaccgcg
    31681 cggatcttgg cctcgctgga caggaggtcc ttgaggtagg agatggtcgc ctcggtttcc
    31741 cgcagctcgg cctcgagctt ctcggtctcc agggaggtga gcttggccag gcgcatatcc
    31801 acgatggcct gggactggac ctcgtccagg tcgaagcgct ccatgaggcc cttcttggcg
    31861 gcgtccacgt ccttggctgc cttgatgatc cgaaccacct cgtcgatgtt ccgcagggcg
    31921 atcatcatcc cccgcaggag gtgggccttc tcctcggcct tgcgcaggtc gaaccgtgac
    31981 cggcgggtca cgacctcgac ccggtgggcg acgaagcagg agacgatgtc cttgagcccc
    32041 aggagcttgg gctgaccctt gaccagggcc aggttgatga ccccgaaggt ggactgcagg
    32101 gcggtgtggg cgaagagttg gttcaggatg agcttgggga cggcgccctt cttgagctcg
    32161 atgaccaccc ggatgccctc ccggtcggac tcgtcccgga ggtcggagat cccctcgatc
    32221 accttgtcac ggataaggtc cgcgatccgg gccaccaggg tggccttgtt caccgcgtag
    32281 ggaatttccg agaagatgat ctgctcccgg ccgcccttct gggtctcgat ctggaacttg
    32341 ccccggacca cgatcttgcc cttgcccgtg acgtaggcct cccggatccc ccggcggccg
    32401 aagatgatgc cccccgtggg gaagtccggg ccctggacat gacgcatgag cccgtccacg
    32461 gagatctccg ggtcgtcgat gaaagccgcc acggcctccc cggcctccag gaggttgtgg
    32521 ggcggcatgt tcgtggccat gcccacggcg atcccggagg acccgttcac caggaggttc
    32581 gggagggccg agggcaggac cgagggctcc ttcagggact cgtcgtagtt cgggacgaag
    32641 tccacggtct ccttgcccag gtccccgagc atctcctccc ccacccggga gagcttggcc
    32701 tcggtgtagc gggaggccgc ggggggatcc ccgtcgatgg agccgaagtt gccctggccg
    32761 tggaccaggg ggtagcggag ggaga
//
LOCUS       DAOTEI010000017        32732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_897_length_32732_cov_5.418735, whole genome shotgun sequence.
ACCESSION   DAOTEI010000017 DAOTEI010000000
VERSION     DAOTEI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 32732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32732
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_897_length_32732_cov_5.418735"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="P5313_02845"
     CDS             complement(<1..1726)
                     /locus_tag="P5313_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY79335.1"
                     /translation="MFDQVRPEDLAYVGKDSGRIDATEKLTGEALYVSDMTVPGMLYA
                     AVKRSPHARARIKRIDTSKALALPGVRAVVTGAELDYRLGLYVVDKYILAKGEVRHYG
                     EAVAAVAAETYAQARRAADAIAVEYEPLPPVLHHMEALKPDAPRVHPDLGSYDHIAAV
                     FTPKPGTNIANHTRLRKGDTEKGFAESEWVVEREYSCPSVQHVPMETHVAIAKWERGD
                     RVTVWTSAQSPFTVRNLMCVAFKLPHNNVRVHIPYVGGGFGGKAGIGVEPLVAVLSRA
                     AGGKPVKFQATREEEFSLLPCRSALTYRLKTGVSSEGKILAQEMTLYWDSGAYADYAV
                     NVTRASGYSASGPYEIPNAKVDSYTIYTNKPYGTAYRGFGHVEFHWALERHMELVAEA
                     IGMDPLEFRRRNALRPGSKTLTGEEITSHTGNVLKCIDKAAASIGYGKLTPAEAAREK
                     KTGWKIGKGVAALQKAPAMPSYTATAVFLKMNEDGTVTVNLALTDYGQGTYTSIRQIV
                     AERLGFPLEKVKVSFECDTDKDPYDWQTVASKGLLLSGNAAILAAEDLLKNAYDTAAR
                     ALRAQECDL"
     gene            complement(1719..2201)
                     /locus_tag="P5313_02850"
     CDS             complement(1719..2201)
                     /locus_tag="P5313_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY79336.1"
                     /translation="MTITLTVNGVSYTRDAPENKTLLHFLREDLGLTGTKEGCGMGEC
                     GACTVFLNGRTVNSCCVLAVEADGAAIETIEGEAGPAGLSEIQKAFDRNHAVQCGFCT
                     PGMILSVKELLKAVPKPTVEEIKEAIEGNFCRCTGYEQIVEAVLDVTGQGPKGRLDHV
                     "
     gene            complement(2198..3016)
                     /locus_tag="P5313_02855"
     CDS             complement(2198..3016)
                     /locus_tag="P5313_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY79337.1"
                     /translation="MVHEFQYRAPRSLSELLALMAEHGTSAKVLAGGTDLLPNIRMGI
                     AKPAFVVDCKRLPDADRLVFSPAEGLRMGHGVTISAVLRDRTIREKFPLLAEAAHELA
                     SHQVRNRATVAGNVVNASPCSDMALPLLCLDAVAVLTSTSGERTVSFREFFTGPKATV
                     MKPGEFLAEIRVPASSADRKGRYAKLKRVKGHDLGIVGVMLASSPEGLRVAVSSAAPT
                     PVLVSGIGRGLDAEAVDALVQKAIKPIDDVRCTAEYRRYMVGAYIRRLLPEVSR"
     gene            complement(3118..4620)
                     /locus_tag="P5313_02860"
     CDS             complement(3118..4620)
                     /locus_tag="P5313_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY79338.1"
                     /translation="MDTGARCSIEVVGDVAVLHVAGAVEMAGADILRDEILARIQGTG
                     LSKVVLDLEKVPLVDSSGLGLFLSLSQQLSESKRIRFCNMAGNVRAVFEYMGIATYLD
                     LDHSQEESLAALSKPAAPAKAARRIVEKPLDLPGKYVLNEAGQRYCSQLRIPVRDLRT
                     FAGDRAVGFDWKVCKLDLLRKLVVHGLITTIEISRPEFVSARHALLDLTRTILSGILL
                     KRFRPELKRRLRRTPEAARIASDPAFIGLVGDRAAMASALRRRAVWAANLRTSIEEEC
                     AARTRSGSPEGTCDEDTLARVSSLLDEVDDETALLLALGGADLVGTAADVVYSYARRL
                     EIAEHLCLMLAEFIQLAEKSFLTNLAERELFVRSHPEELERMLAEEAFRDRLRERAVQ
                     RNELMLLRMDFTGMVLDPSDPAAIRLTVRNRGLIGYGSRLENLGRRPRTVKENTLEQI
                     LKADEEGGGLGLIYHTLLREKCAAEGMDFSTSVIRNEKEDETVATLNLTV"
     gene            4711..6552
                     /locus_tag="P5313_02865"
     CDS             4711..6552
                     /locus_tag="P5313_02865"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRY79339.1"
                     /translation="MQITYMNGKRLKRALLAGALRLGENVPHLNRINVFPVPDGDTGT
                     NMAGTVQAMASALHASPAISLGETARTAADSALMGAKGNSGAILAQFFQGLAEELERK
                     VRVSTRDFADAVGKAVARTYGALSKPREGTLLTVLKDWGHAVHRVAERTDDFLELLKT
                     TLHDARASLERTRDMLPELKKAGVVDAGALGWVHILEGIVHYITHGRLREFRDSPPEG
                     FSETYEESHVESPLADSEPSAFRFCTECLLEGSALDLEAVRRGLEGLGDSIVVAGSAR
                     KAKVHLHTDTPARAFRVLEGFATVTGQKADDMELQRRLAADPPRCAVFVDSACDLPEE
                     ERLALRVERVPLTIVMDGVNYLDRDGLSSEDFYARQRTPGIALSTSQPSDAAFRRKAD
                     TVLASAREAVYLGLSGPLSGTLEAGRRTAAARPDSGRIRVVDSRSISVGYALLARKAG
                     EAAAAGASAEEIGTLVESLKPRLRLLVAVPDLSGLIRSGRLHGMKGLAARAFGLRPVI
                     TVNEEGRADSAGMYVGAANGRAALVRMLRKAVSGAGAPETGSAPLEILVGHADSRAEA
                     EALVRDLGAAFELSREPDIVEVSPALALHAGPGALALAALLPVGRVR"
     gene            6549..7208
                     /gene="plsY"
                     /locus_tag="P5313_02870"
     CDS             6549..7208
                     /gene="plsY"
                     /locus_tag="P5313_02870"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRY79340.1"
                     /translation="MIGPAAALVLSYLLGSFPTSIVVGKVFFRRDIRAEGSGNAGGTN
                     TFRVFGWKAGVLVVLVDVGKGVAAALLASRLAAGTPLPEDAVRLACGTAATVGHVWTV
                     FAGFRGGKGVATAAGTLAAVAPLPFLAALAAFALGILSTGIVSVGSLAAAVTFPAAAL
                     AIRAAGFPVSNVLLGFSFLLAPLIIWTHRKNIGRLLRGEENRFEKLRVLPRLFRKQDL
                     K"
     gene            complement(7367..8257)
                     /locus_tag="P5313_02875"
     CDS             complement(7367..8257)
                     /locus_tag="P5313_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRY79341.1"
                     /translation="MKAGRANLGIGILLFAVAAGSFGQVSEDEVRRRILEAASRFRGV
                     PYMYGAESPAAFDCSGFVRYVYRQAAGIELPRSSRGIFAAGIPVDPRKARPGDVLVYD
                     TVGGYPSHVAIYLGEGSVIHAVSQGPRTGVIVSPASDSYFPPRLIAARSFLGSPGSAG
                     ALVRPPAAASAPAAAAPAAAPAAPSREEPPVADIGFTIPRERASYADPIPAAAGTRIS
                     FTLTNGTGKDGAFVVIFYKADPQFRESQEIHRETARIPAGGHLELPAYLFAEPGQYKL
                     IVKDTWNTQLLERTFAVKGK"
     gene            complement(8254..9234)
                     /locus_tag="P5313_02880"
     CDS             complement(8254..9234)
                     /locus_tag="P5313_02880"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HRY79342.1"
                     /translation="MADMKTKYLGLTLKNPVVVAASSLTATPTGVKKAADAGAGAVVL
                     KSLFEEQLRADIQSVGGSAGESHPEADAYLAEMGMHAGSGEYLKLIREAKAAADIPVI
                     ASVNCIGGPWWTEFAEQVGKSGADALELNIAFLSTSPDETASSLEDRVVQIVRTARAA
                     SGLPVSVKLGSQYTNLLNLASRLSSAGAGGLVLFNRFFRMDIDLSTLGLMAGPVHSKP
                     EDYHESLRWISLLFGRVPCDLAAGTGVHDYASALKVILAGARAVQVCSVVYQKGYGVI
                     REILTGMETWLDQRKAAGLDDIRGKLSRFTSRSPADYERLQYIKALTGIY"
     gene            complement(9481..10296)
                     /gene="nudC"
                     /locus_tag="P5313_02885"
     CDS             complement(9481..10296)
                     /gene="nudC"
                     /locus_tag="P5313_02885"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRY79343.1"
                     /translation="MQDTQPKPAYFAFRGQELLCRPDGRAEPLDSHPFPDRKPGKDPW
                     LLDVFPLRIPGPADPAKEGPVPTVLSLAPEAEAPEGLSWVPFRSVLGSLAWDGVLPAC
                     RALALANWRAGSRFCGRCGSAQGDKPDETARLCPSCGSVTYPRLSPAVLARVHRDGRI
                     LLARNAAFKTGIFSVLAGFVEPGESFEDCVVREVAEEVGIRVRNVRYLGSQPWPFPDS
                     LMVGFEAEWESGELAPDGIEIAEAGWYGPDDHPPLPLPGSLSRRIIDAELFKK"
     gene            complement(10372..11529)
                     /locus_tag="P5313_02890"
     CDS             complement(10372..11529)
                     /locus_tag="P5313_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79344.1"
                     /translation="MPTRSSRSRGTNPAAARAFTAGGGAGAAASVRALLPAAVLAYAF
                     LGEPGAALTAAAAWALSLAGEAWSRGAARQAPVADSSGAPTSGPAKGSGRPAAGSGRP
                     VPGVSSFRPFLPAGIAFVSFQAVFLASDLLRGGFPIRSAFLDSIQSALTCTVLLLPLG
                     LPAAAAFLRSRTGRDGFSRVEAFQFTCCAGMSLAVAGSVLAGWLPPLSTAQLLWANAV
                     GTVLPTLSLAFVRGDAGPTAPTEGERPSRIIPDILVPWLLLALLLLAVYRLGLGYAGV
                     APRAADRARSLVFLALCLVQAGEPPGRLRSPPESGNPRARARRIAVRLGVFLAALVPA
                     AAGFLVPAARTFLGLSVPEPAAAASALGAAGMYAVLRFAARRIRSPIPRKN"
     gene            complement(11537..12502)
                     /locus_tag="P5313_02895"
     CDS             complement(11537..12502)
                     /locus_tag="P5313_02895"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY79345.1"
                     /translation="MLAAAAALAALLSSCATAQAPRIALEYYEIGNAWLAMEKWDKAG
                     AAYERALAYDRSLVAASYNLARALAEAGAYPRALEVLERLRSDDPDNVRLIAFRAYVL
                     NKSGDDEAALAAYRRAAELNPRDAGTLRNLALLLERAGRDGEALVILRELYAVNPADD
                     GVLRRLALLEARRVSGTRGSGESPAPGEESAPAITSTAAAAASAEEDALTLLSAYLSR
                     KPGDREVLRARAELREARELFARAQEDWAALAQADPADGEAWFRLARIRLVVAGDREA
                     GMEALRKALDAKFSDSKAAAALLAALPEAERAKPAELLKAAGLSE"
     gene            complement(12535..13599)
                     /locus_tag="P5313_02900"
     CDS             complement(12535..13599)
                     /locus_tag="P5313_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRY79346.1"
                     /translation="MNRVILKAQDLDDYLTEGDQKFLGQLDVLYRQAMVSFNILSHAK
                     GADERRREEKVLIDLYNRMGGAMQELCKAEPRAQVYSFVTPSENHGEASRLIAKLRDA
                     RTQRFEFVYYIQRAYELLFNLAFGGARSENKNHLIVSTPVTVPVQNYAVHKIPNIDDR
                     IENTVMCVMLRGALLPSMILSKEIQEYSSSGYVTPFALFKISRDDTKVENNMEYVLDL
                     DRSYFRTEDMDGKDLLFADPMNATGGSLVTVVKYLLDLGVKPRSIKVFNVISALKGAL
                     RVVRAIPEATVYTLWMDPVLNEMAYIMPGLGDAGDRVNGVDEHERPRDMLQLIADYGS
                     NIANLYRSQIRTIEEMVLRR"
     gene            complement(13596..14174)
                     /locus_tag="P5313_02905"
     CDS             complement(13596..14174)
                     /locus_tag="P5313_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HRY79347.1"
                     /translation="MPENKVLINLSNRHVHMCREDVEILFGKGHQLTKMKDLLQPGQF
                     ACEETVTIKGPKGSFEGVRILGPERKETQCEVMASDAFRLGLRELPCRESGQLDGSAA
                     FEIVGPAGSVRKTQGLIIAKRHIHFDPASAERFGVKDKQIVKLHAGGDRGATFDNVVC
                     RVNAAYALECHLDFDEGNAVGIGNGAQGEVVL"
     gene            complement(14286..>14475)
                     /locus_tag="P5313_02910"
     CDS             complement(14286..>14475)
                     /locus_tag="P5313_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79348.1"
                     /translation="DDLRSLYPRLVGKGGRGWAAWGLVLDGRRDLAVRADPPRRAEAV
                     RNQAGAVAELLGRELRER"
     assembly_gap    14476..14485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14486..14817)
                     /locus_tag="P5313_02915"
     CDS             complement(<14486..14817)
                     /locus_tag="P5313_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79349.1"
                     /translation="MSPFSPPLVLRPKAPGRVDLAVHPLVRAAQAAFAAAFALGSLGS
                     GDPFWLAGTAVCLLAALSEDSWILDSARRIIRRRFGILPFPRSFDLDWEEVEAVFVET
                     VDRPTAED"
     gene            complement(14814..16442)
                     /locus_tag="P5313_02920"
     CDS             complement(14814..16442)
                     /locus_tag="P5313_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY79350.1"
                     /translation="MSQESRGQPLEAQAAALLKELRSRYRVLADHVWSLSIESDLTTD
                     FFEESLAANSKNLDSLRAIEADLESLNGGISGISGAAGEADARLEAAESSVGSARGAV
                     EDGTKALADMDSRFELFISMFRRVAESADRIEEALQSIEEIAELTNLLALNAAIEAAR
                     AGVHGRGFKVVATEVKALAAKSRALTDTAAGLLKGLRAGMGSADEGLAAMAQGKAELS
                     RRMAHARDELGSGASAVAGAASNMRSIRGALEGQKESSGRIARSMTALSEAARLLNDN
                     SALVRGNLERQKASSEAVQSAAGAVKTEINHMATSFADLFGSRACGETIPIAHDVSYP
                     PWVHIQGGQSAGKAIDTARRALEAAGLVPEFHPGQFFEALSDLLAGKVRVVANVGWPN
                     QGLADKPVIPTRPFGVFRPAVFAHTSRTASFRGLPDLSGRRVAAQEGSYVVDCLRGTG
                     CEVVTTANDLEAFAAVIWERADCAITERLVGAYLSENYFSGTVKACFETGHELSVVYL
                     LSRGDEALRDAFDKGLAVLGTSRAAEELAGRERP"
     gene            complement(16592..17242)
                     /locus_tag="P5313_02925"
     CDS             complement(16592..17242)
                     /locus_tag="P5313_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY79351.1"
                     /translation="MIRCLLFDLDNTLYPSSNPMESQIVGRMNEYTARFLNLSVEEAL
                     DRRRADMPRHGTTLEWLMAEYGFDDPEDYLAAVHPEGEEECIDPDPSLGDFLRSLPYP
                     KVVFTNSTREHADRVLGRLGFPDVFQAVFDIRFNHFKGKPHPAAGLRVCSSLGVKPSE
                     TLLVDDLPRYVRGYLECGGNGVLLDDRNRHPDYDSLRIASLYDLASLLSDGRFGSV"
     gene            17338..18465
                     /locus_tag="P5313_02930"
     CDS             17338..18465
                     /locus_tag="P5313_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY79352.1"
                     /translation="MQRILYLYLNTGGGHISGARALKAGMENLFPGRAEGHLLHGYPP
                     RLVLDRLVMEQGYSLAINHFMPGYLLVYKLAELRPLITLSQETTAQACAGYIASHIRR
                     LGITKVVCLHFILEKAARDAIRQVDPAMPLVTVVMDPFSVPPIWFGVKDQEFCVFSER
                     ARRSALDEGVAPERVRVFPLMLRKEFERPRPPGEIPELKRRYGFDPDRPLLLLAGGGD
                     GLPRSDRIVREALKDGFEGQIAVVCGKNMLLRRRLRDLRSRLGARDLKIYGFVDFMAD
                     LMAMADCVVTKAGPATVMETLALRKPLILSCYVRNQEKGNVQFVTSNKVGWYLTEPSD
                     IVGKTREIFGKPELRRELEGRIAGLGIRNGLSDLARHIFEL"
     gene            18547..19050
                     /locus_tag="P5313_02935"
     CDS             18547..19050
                     /locus_tag="P5313_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79353.1"
                     /translation="MNRSKFCIPALFILALASAPAFGAPEKKLDVDLSPSNWALGIFL
                     GEPTGITVQLDVGATQALEVKAAWNFVAAGQNEGGNVTLQGNYVLWFPGLLVFDGHDI
                     PPFAGIGVEARFGTDFLLGLHIPFGVRYRFREVPIELALELGAGMSLIPSTSFMGSGG
                     LAIRWML"
     gene            19066..19848
                     /locus_tag="P5313_02940"
     CDS             19066..19848
                     /locus_tag="P5313_02940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY79354.1"
                     /translation="MIPVGDDNSRNRRTPVVTRLLLALNIAAFILLQQCGTDPNIPRS
                     LGAVPQELIQGRDIRTADQVIRSPTTGRSYRVPGLLETPVWIYLTVLTAMFLHGSTGH
                     ILGNLLYLGIFGDNVENRMGPMRFLLFYLIMGAAATAAQVLASMVTGQGLLTPMIGAS
                     GAISGVMGAYLVLFPRNRVYILTFGFYPMPVSAWFALGIWFLMQLVSASAAGPAGSGV
                     AYAAHIGGFAAGWLWARIFEAGDSRRRGRGGRDGDITWRIVD"
     gene            20024..21829
                     /locus_tag="P5313_02945"
     CDS             20024..21829
                     /locus_tag="P5313_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79355.1"
                     /translation="MAQDKKTSGRRNPVRFFTYLSPYWNLFVAGFAAMLAVTAARLAG
                     PLILRELVDKAIPARDTGLMLRYALAYMGIIVVMGALTYFQTMLIVRLGLKVVTRIKD
                     ELFSHLLTLPVSYFDKHPVGELMARVENDTEKVKQLFSETVIFLATNVLYFLGMFAVF
                     FRMDARVASFILLPVPLFLAAFLFLFGRLRPLYEKARKKYAEITAVATEFVQGIEILR
                     AFNRTGYAAERLDAASKEKRDVEIRAGLFEYGFTGGMGFLAGPVFVVLIIRLVAPNIF
                     RGALTVGTLLVFIEYGMRLFEPLLAIGENIRGIQQARVALGRIFGILDLEPEESPDAG
                     GDPPSFRKSITFDRVSFEYKEGEPVLDSVSFTVEKGQTVALVGASGSGKTTTVSLLCR
                     FYPYSRGRILVDGRPLESLDLRAWRRMIGLVLQDIYLFPGTVLENVRVYDDTVTELDV
                     FGALKTVHAAEFVRRLPNELRTELHERGANLSMGEKQLLSFARAVAFDAEIVIMDEAT
                     ASVDVATERRIQESMAELLAGRTAIIVAHRLSSILKADKILFFKDGRIVAQGRHEELL
                     ASLPDYGDLVRLQFPDLEGGGRGAEKRIAAVETSA"
     gene            21826..23592
                     /locus_tag="P5313_02950"
     CDS             21826..23592
                     /locus_tag="P5313_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79356.1"
                     /translation="MSARGTVDHVAWIRAVWSEHKGLVALLFFLTLLSSAVAVLYPYL
                     SKLLLDTIQGLLASPGLGNPMDEVDRLLLVFLAVGLAGLVASMFPGIRGMTNSVFEHV
                     IRSKYFRKVLAKDYSFFAKFSTGDVVTRLTDDLYDFPKLSWFLCSGIFRAVESISKVA
                     FCLAAMVLLNPKLTLYSLVPLPLMIAIFYVTQDRIYDTFQKNQEAISAVNARLEMSFS
                     GVRIIKAYACEDKYRRFFRNVLEQRFGTELAVAKLEVVLHLIYQYIDYVAQVGIVFAG
                     GLMAVRGEISIGTFYAFYTYLTLLIFPILDIPQLFVSGKRAFVNIDRLEEIARHPEPA
                     APGKPRAVDRLERVELYGVSFRYGDRAENSLTDICFSIGRGERIGVIGPVGSGKSTLV
                     KCLLGLLPPSSGEIRVNGEDLRELDLVSYRARVGYVPQEALLFSGSLRENVDFGGESL
                     SDEMFRTSVEAAQMAEEIASFTDGERTVVGQRGVSLSGGQKQRMAIARAVARRPELML
                     FDDITASLDAANEERLMRRLDELSGDLSFVIVSHRLSTLQYVDKVLFLDQGRALGFGR
                     HEELLQNRRYRAFIEEHLGKSE"
     gene            23674..24207
                     /locus_tag="P5313_02955"
     CDS             23674..24207
                     /locus_tag="P5313_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY79357.1"
                     /translation="MKTGALYHVTARANRQEMILDSRGMKELFMKTLSRAKKKYRFRV
                     ENFCVMGNHFHLIIRPEAGESLSRILQWILSVFAMAYNRIHGLWGHVWGSRFFSRILA
                     GLREYLAVFDYIDQNPLAVHRVRDPREWMYGGLWEHRHGYRTVADRPEPYVEMMVPDH
                     GLVRLERVTPRRHAHFS"
     gene            24361..25509
                     /locus_tag="P5313_02960"
     CDS             24361..25509
                     /locus_tag="P5313_02960"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY79358.1"
                     /translation="MERLQTLLDTLVSHQPIKQAIMAVESGDGSFRWSGAAGKSVDGL
                     PLDPEAPFFIASIDKLYNAAVVLMLAERGKLRLDDPISDYLPVDLTRGLHTLNGIDYS
                     GSLTIRHLLSHSSGLADWLEDTPKNGKNLIEHVLERDDRSMSTAEIADLVRGLSPHFP
                     PQDPSARRVKIRYSDTNFILIIALLETMCGQPLHRIHEDLLYAPLGLRHTYFPGTTLP
                     LETTSAPVPLRAEGRIIEIPRLMTAFRGIYSTAGDTISFLRHLACGEVFQNPKTFALM
                     QSRWNRFGFPMDRAALRSPGWPIEYGLGLMRFQLPRAFTSLKRLPAVIGHTGSTGCWL
                     FWCPEMDLYFSGSVDEAGAGAVPYRIIPQILQILATGEGGPIHGIRKV"
     gene            25490..25951
                     /locus_tag="P5313_02965"
     CDS             25490..25951
                     /locus_tag="P5313_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79359.1"
                     /translation="MEYAKFSLLFMLVHTGAYFIAGMLAYRVSRDLYMGENRLLDFVK
                     DMSDQGENASVAKLTLPLQLVRGLVLSIVLYPIIGFLGELSYLHRFLFLTGLMFVYTD
                     FASAIPFPHNLEGLIYLKARYFKKFAFWKLQFEMIVFSLIFGLASGWILFQ"
     gene            26000..26890
                     /locus_tag="P5313_02970"
     CDS             26000..26890
                     /locus_tag="P5313_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79360.1"
                     /translation="MQRSLTWIVPLVAVLALAASATGLFLPGGDGPYKFTTLRGQEVE
                     MFGRGLYRYDTLLVGAALRGTDAVTLLIGLPLLVLTYMKSRHGSTNAPLALLGVLFYF
                     LYKGASLTFSAAFNPLFLVYTALFSASLFAVIISMTAFDLESLAKRVQPGFPHRGAAV
                     FLFIAGIGTLLIWLSEIFVPLLAGTAPEIMGPYTTLFTHGFDSAVITPAAVIAGVCLV
                     RRKPLGYLLTIPILILCTLIGAVVIGQSVAQTLVGLVFPPQIYIGMIGSWIVMGGFAI
                     GLTASSFRNLEPPSGDRVRS"
     gene            complement(26921..27334)
                     /locus_tag="P5313_02975"
     CDS             complement(26921..27334)
                     /locus_tag="P5313_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRY79361.1"
                     /translation="MKSYRKEFMIHTKERYAIVRITDRVEEALRESGIREGLCLVNPM
                     HITASVFINDDERGLHSDFLAWLEKIAPYDRNGYRHNTGEDNADAHLKRAIMGREAVV
                     AVTDGRLDFGTWEQIFYGEFDGRRDKRVLMKIIGE"
     gene            27489..28259
                     /gene="recO"
                     /locus_tag="P5313_02980"
     CDS             27489..28259
                     /gene="recO"
                     /locus_tag="P5313_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRY79362.1"
                     /translation="MPHRNLSFEAVVLRCRESPGGDRIVTFLAANSGLVEAFVFGGPK
                     SRLRSLAAPYHTGTAWIYLDPVKDLRKLSDFDARETYPTLRENLRKIWAASYFAETVL
                     RTDAAGGESREAFDLLRSGLSALDAGGEADVDYVCLQYGWRLLGLMGLRPDSETCGQC
                     GASFPEGRRAAFSRIQETFLCDQCARAGDSGNSVDDLTLSGGALRYLAASEALPFRDA
                     LRVRPSGEDLPILKALVFHLVRSAAEGELRTLARGELL"
     gene            28256..28846
                     /gene="thpR"
                     /locus_tag="P5313_02985"
     CDS             28256..28846
                     /gene="thpR"
                     /locus_tag="P5313_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRY79363.1"
                     /translation="MRCFIALELPDEAKVRLAAAAGILAGRFPGARWVRPESFHLTLA
                     FLGEIKGTELDCARDAVVATAGSGAFDLAFAGTELLPVRGPARVLALSAGAGTRECVE
                     IHGRVNRALSLAARAAGLPPLNPEWPDGRPFRVHVTLARAGGRPFPRDPRGLGEAWEE
                     IRACTQAPCRISRCILYRSAPGPGGARYEPVLATVL"
     gene            28866..30176
                     /locus_tag="P5313_02990"
     CDS             28866..30176
                     /locus_tag="P5313_02990"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRY79364.1"
                     /translation="MRAVDLIMKKRDGGELSREEIAFLVSGYVDGSLPEYQVSAWLMS
                     VFFRGMNPRETADLTELMLRSGDVMDLSGIPGPFVDKHSTGGVGDKVSLILAPIAAAC
                     GVKVPMMSGRALGHTGGTLDKLESIPGYRTNLSPAEFRDFLRADGYAMTGQTARVVPA
                     DKKLYALRDVTATVESVPLITASILSKKVAEGAESLVFDVKCGPGAFMKTFPDARRLA
                     ESLVDTGKAMGKRIVGVITDMTEPLGLKVGNFLEVEESLDCLTGKGPADLMEVTYRLA
                     AWMLVAAGKAKGVEEALRLCEAAVAEGRAMELFRRNVRTQGGDLAKMDALYGKLRSPH
                     SFELRAPASGFVTAIDAYRIGLAGVYLGVGRNTTADPVYPDVGFVFERKVGDSVTAGD
                     LVARVYGKDEASLAAARPLVEEALTVGPAAPAPRKLVLEELAAL"
     gene            30173..32308
                     /gene="recJ"
                     /locus_tag="P5313_02995"
     CDS             30173..32308
                     /gene="recJ"
                     /locus_tag="P5313_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRY79365.1"
                     /translation="MKWNKKDVSPQAVKDLAARFDLDLLSASVLARRGVTDPGELLYF
                     VEDDPRYLHNPFLFDQMEDAVDRVLLARDEGEKVLVFGDRDADGISSTVLVYEALKSR
                     GLDVRYRLPSADDPYGLSAKAVEEFAGDYGTLIITVDCGISNHAEIERARELGVDVIV
                     LDHHLHRRDELPGAVAVVNPKIESCGYPFRDLAGCGVAYKFTRALAFAESGLYKQQVA
                     LLNVRPANDAYTLEAVRLTNLVETGRISETIVPGMVELEKTRLVPFLKDRQIFVWDGD
                     LQKKLLAKAFGRAADVQVYDIAEDIGDLIPAVRGKSLLRLREQSRIAKYSDKAAGEID
                     VFASLFVSFALKKTSYFGEADLEALQLVAVGTLADLMPLRNENRILVRKGIAALNAKP
                     RAGLAELLAKLNLTGKRLAAKDLAWQLTPAINATGRMGSPETAVRLFLGQTVEERSRL
                     AEEVIGMNQDRRRLGAEGWEAVLPDARKSLEAHDNKFVLVGRDDVNRGITGIIAARLS
                     NTLRVPAIVAAFLGGGTIVGSLRSLRGYPVKALLEACSDLFIDYGGHDEAAGFSLPAD
                     KWDELTRRVLEFTKAVELEAEEESLSIDAELPHDYMKPEITDLVDRFEPYGEENGPLV
                     FLTRDVPIASMELVGKKEQNHVKMLLDYGKHRWPALFWSAAERVERDFSRTDRVDVVF
                     QVGRNYWNGMEQAQLVVMDVRRTGEDGTH"
     gene            32359..32429
                     /locus_tag="P5313_03000"
     tRNA            32359..32429
                     /locus_tag="P5313_03000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32391..32393,aa:Gln,seq:ctg)
BASE COUNT         5295 a        10983 c        10839 g         5605 t
ORIGIN      
        1 ccaggtcgca ctcctgcgcg cggagggccc gcgccgccgt gtcgtacgcg tttttgagaa
       61 ggtcttcggc cgcgaggatg gcggcgttgc ccgagaggag gagccccttc gacgcgacgg
      121 tctgccagtc gtaggggtcc ttgtcggtgt cgcactcgaa gctgaccttg accttttcga
      181 gcgggaagcc gaggcgctcc gccacgatct ggcggatcga ggtgtaggta ccctggccgt
      241 agtcggtcag cgcgaggttg acggtgacgg tcccgtcctc gttcatcttg aggaagaccg
      301 cggtggccgt gtacgagggc atggccggcg ccttctgcag cgccgcgacg cccttgccga
      361 tcttccagcc ggtcttcttc tcgcgcgcgg cctccgcggg cgtgagcttc ccgtagccta
      421 tcgaggccgc cgccttgtcg atgcacttga gcacgttgcc cgtgtgcgag gtgatctcct
      481 cgcccgtgag cgtcttcgag ccggggcgga gcgcgttcct ccggcggaac tcgagcgggt
      541 ccatgccgat ggcctcggcc accagctcca tgtggcgctc gagcgcccag tggaactcga
      601 cgtggccgaa gccgcggtag gccgtgccgt agggcttgtt cgtgtagatg gtgtacgagt
      661 cgaccttggc gttcgggatc tcgtagggac ccgaggcaga gtagcccgag gcgcgcgtca
      721 cgttgacggc gtagtcggcg taggcgccgg aatcccagta gagggtcatc tcctgggcga
      781 ggatcttgcc ttcggacgac acgccggtct tgaggcggta ggtcagcgcc gagcggcagg
      841 ggagcaggct gaactcctcc tcgcgcgtgg cctggaactt gacgggtttg ccgcctgccg
      901 cgcgagagag caccgcgacg aggggctcga cgccgatgcc cgccttgccg ccgaagccgc
      961 cgcccacata ggggatgtgc acgcgcacgt tgttgtgggg cagcttgaac gcgacgcaca
     1021 tgaggttccg cacggtgaag ggcgactggg cgctcgtcca gaccgtgacg cggtcgcccc
     1081 gctcccactt ggcgatggcc acgtgggtct ccatggggac gtgctggacc gaggggcagg
     1141 agtactcccg ctccacgacc cactcgctct ccgcgaagcc cttctccgtg tcgcccttgc
     1201 gcaaccgggt gtggttggcg atgttcgtgc cgggcttcgg ggtgaagacc gcggcgatgt
     1261 ggtcgtagct cccgaggtcc gggtgcaccc ggggggcgtc gggtttgagg gcctccatgt
     1321 ggtgcagcac cggcggcagc ggctcgtact ccacggcgat cgcgtccgcg gcgcggcggg
     1381 cctgggcgta ggtctccgcg gcgacggcgg cgacggcctc gccgtagtgg cggacctcgc
     1441 ccttggccag gatgtacttg tccaccacgt agagccccag tcggtagtcc agctccgccc
     1501 cggtcaccac ggcccggacc ccgggcaggg cgagggcctt ggaggtgtcg atgcgcttga
     1561 tccgggcccg ggcgtgggga ctgcgcttga ccgccgcgta gagcatcccc ggcaccgtca
     1621 tgtcgctgac gtacagggcc tccccggtca gtttttccgt ggcgtcgatg cggccggaat
     1681 cctttccgac gtaggccaga tcctcggggc ggacttggtc aaacatggtc gagcctcccc
     1741 ttcgggccct ggccggtgac gtccaggacg gcctccacga tctgctcgta cccggtgcag
     1801 cggcagaagt tgccctcgat ggcctccttg atctcctcca ccgtgggctt aggtactgcc
     1861 ttgagcagtt ccttgacgga gaggatcatc ccgggagtgc agaatccgca ctggaccgcg
     1921 tggttccggt cgaaggcctt ctggatctcc gacaggcccg cgggccccgc ctcgccctcg
     1981 atggtctcga tcgcggctcc gtcggcctcc acggccagga cgcagcagga gttcacggtg
     2041 cggccgttca ggaagaccgt gcaggccccg cactccccca tgccgcagcc ttccttggtg
     2101 cccgtaagtc ccaggtcctc cctaaggaag tggaggaggg tcttgttctc cggggcgtcc
     2161 cgggtgtagg agacgccgtt cacggtcagg gtgatggtca tcgggaaacc tccgggagca
     2221 ggcggcggat gtaggcgccg accatgtagc ggcggtattc ggcggtgcag cgcacgtcgt
     2281 cgatgggctt gatggccttc tggaccaggg cgtcgaccgc ttccgcgtcc aggcccctcc
     2341 cgatcccgga gaccaggacc ggggtgggcg ccgcggagct cacggccacc cggaggccct
     2401 ccggcgagga ggccagcatg acccccacga tcccgaggtc gtggcccttc acgcgcttga
     2461 gcttggcgta ccggcccttg cggtcggcgg aagaggcggg cacccggatc tccgccagga
     2521 actccccggg tttcatgacc gtggccttgg ggcccgtgaa gaattcccgg aagctaaccg
     2581 tgcgctcccc cgaagtcgag gtcaggaccg ccacggcgtc caggcacaga aggggcaggg
     2641 ccatgtccga gcagggggag gcgttcacca cgttccccgc cacggtggcc cggttccgga
     2701 cctggtggga cgccaactcg tgggccgcct cggccaggag ggggaatttc tcccggatcg
     2761 tccggtcccg gaggacggcg ctgatggtga cgccgtgacc catgcggagc ccctcggcgg
     2821 gggaaaaaac caatcgatcc gcgtcgggca ggcgcttgca gtccaccacg aaggcgggct
     2881 tggcgatgcc catccgtata ttcggcagga ggtcggtccc gccggcgagt accttggccg
     2941 aagtcccgtg ctcggccatc agggcgagca gctcggacag ggacctggga gcccggtatt
     3001 gaaactcgtg caccatcggg aagggtcctt tccgcgggaa tgcccgatcc cgcgcgtgcc
     3061 gtcgattttg acatcgaccc atcggtctgt caagaatttc ttgagcgcgg ctatcgtcta
     3121 cacggtgagg tttagggtgg cgaccgtctc gtcctccttc tcgttccgga tcaccgaggt
     3181 cgaaaagtcc ataccctccg cggcgcactt ctccctcagc agggtgtggt agatgaggcc
     3241 cagccctccg ccctcctcgt ccgccttgag gatctgctcc agggtgtttt ccttgaccgt
     3301 ccggggacgc cgccccaggt tttccaggcg ggacccgtag cctatgaggc cccggttccg
     3361 gacggtaagc cggatggccg cggggtccga ggggtccagg accataccgg tgaagtccat
     3421 ccgcagcagc atgagctcgt tgcgctggac ggcccgttcc cggaggcggt cccggaaggc
     3481 ctcctcggcc agcatccgct ccagctcctc cggatggctg cgaacgaaga gctcccgctc
     3541 ggccaggttc gtgaggaagg atttttccgc cagctggatg aattccgcca gcatcaggca
     3601 caggtgctcc gcgatctcca ggcgccgggc gtaggaatag accacgtccg cggcggtccc
     3661 caccaggtcc gcgcccccca gggccaggag cagggcggtc tcgtcgtcca cctcgtccag
     3721 gaggctggag acccgggcca gggtgtcctc gtcgcaggtt ccctccgggg agccggagcg
     3781 ggtgcgggcc gcgcattcct cctcgataga ggtgcgcagg ttcgcggccc agacggcccg
     3841 gcggcgcagg gcggaggcca tggcggcccg gtcgccgacc agcccgatga aggcggggtc
     3901 cgaggcgatc cgggccgcct cgggggtgcg ccgcaggcgg cgcttgagct ccggccggaa
     3961 ccgtttgagc aggatgcccg aaaggatggt ccgggtcagg tccagcaggg cgtggcgggc
     4021 ggacacgaac tcgggtcggc tgatctcgat ggtggtgatg aggccgtgga ccaccaactt
     4081 gcgcaggagg tccagcttgc agaccttcca gtcgaagccc acggcccgat ccccggcaaa
     4141 ggtccggagg tcccggacgg ggatgcgaag ctgggagcag tacctctgcc cggcctcgtt
     4201 caatacgtac ttccccggaa ggtccagggg tttttccacg atcctgcggg ccgccttggc
     4261 cggcgcggcg ggtttggaaa gggcggccag ggattcttcc tggctatggt ccaggtcgag
     4321 ataggtggcg atgcccatgt actcgaagac ggcgcggacg ttgcccgcca tgttgcagaa
     4381 ccggatccgc ttggattcgg aaagctgctg ggacaggctc aaaaagagcc cgagcccgga
     4441 gctgtccacc agggggacct tctccaggtc caggacgacc ttggaaagcc ccgtgccctg
     4501 gatccgggcc aggatctcgt cccggaggat atccgcgccc gccatttcga ccgcccccgc
     4561 cacgtgcagg acggctacgt ctccgactac ctcgatcgag caccgggctc ccgtatccat
     4621 gctacatgat gatgcacagc cccgacgcta cgtcaagacg attcggaatc ccttgattca
     4681 tcgagtttta catggtacct tgaaagtgtc atgcaaatca cctacatgaa cggaaagcgg
     4741 ctcaagcggg ccctcctggc cggagccctg cgcctgggcg agaacgtccc ccacctcaac
     4801 cgcatcaacg tcttcccggt cccggacggg gatacgggga ccaacatggc cggcaccgtc
     4861 caggccatgg cctcggccct ccacgcctcc ccggcgattt ccctgggcga gaccgcccgg
     4921 accgccgcgg attcggccct gatgggcgcc aagggcaact ccggggccat cctggcccag
     4981 ttcttccagg gcctcgcgga agagctggag cggaaggtcc gggtaagcac ccgggacttc
     5041 gcggacgcgg tgggcaaggc cgtggcccgt acctacggag ccctgtccaa accccgggaa
     5101 ggcaccctcc tcacggtcct caaggattgg gggcacgcgg tgcaccgggt cgccgagcgg
     5161 accgacgact tcctggaact cctcaagacc accctgcacg acgcccgggc ctccctggaa
     5221 cggacccggg atatgctacc ggaactgaag aaggccgggg tcgtggacgc cggcgccctg
     5281 ggctgggtcc acatcctgga gggcatcgtc cactacatca cccacggcag gctccgggaa
     5341 ttccgggaca gccccccgga aggattctcc gagacctacg aagaatccca cgtcgagtcc
     5401 ccgctggcgg acagcgagcc ctcggccttc cggttctgca ccgagtgcct cctggagggt
     5461 tcggccctgg acctcgaggc ggtccgtcgc ggcctcgagg gcctggggga ttcgatcgtc
     5521 gtggccggat ccgcccggaa ggccaaggtc cacctccaca ccgatacccc cgcccgggcc
     5581 ttccgggtcc tggagggatt cgccacggtt acgggccaga aggcggacga catggagctc
     5641 cagcgccgcc tggcggccga tccgccccgc tgcgccgtgt tcgtggactc cgcctgcgac
     5701 ctccccgagg aggagcgcct tgccctgcgg gtggagcggg ttcccctgac catcgtgatg
     5761 gacggggtga actacctgga ccgggacggc ctttcctccg aggatttcta cgcccgccag
     5821 cgcacgcccg gaatcgccct gtccaccagc cagccctcgg acgcggcctt ccgccgcaag
     5881 gcggacaccg tgctggcctc cgcacgggag gcggtctacc tgggactgtc gggccccttg
     5941 tccggcactc tggaggcggg gcggcgcacg gctgccgcgc ggccggattc cggccggatc
     6001 cgggtggtcg actcccggtc catctccgtg ggctacgccc tcctggcccg gaaggccggc
     6061 gaggcggccg ccgcgggcgc ctccgccgag gagatcggga ccctcgtcga gtccctgaag
     6121 ccccggctcc ggctcctcgt ggccgtcccg gacctctcgg gcctgatccg gtccggacga
     6181 ctccacggga tgaagggtct ggcggcccgg gctttcgggc tccggccggt catcacggtg
     6241 aacgaggaag gccgcgcgga ttccgcgggc atgtacgtgg gcgcggcgaa cggccgcgcc
     6301 gccctggtcc ggatgctccg caaggcggtc tccggtgccg gagcgccgga aacgggatcc
     6361 gctcccctgg aaatcctggt gggccacgcg gacagccgcg ccgaggcgga ggccctggta
     6421 cgggatctgg gcgcggcctt cgagctctcc cgggaacccg acatcgtgga ggtatccccg
     6481 gccctggccc tccacgcggg ccccggggcc ctggccctgg ccgccctgct cccagtggga
     6541 agagtccggt gatcggtccc gccgcggccc tggtcctctc ctacctcctg ggctccttcc
     6601 cgaccagcat cgtggtcggg aaggtgttct tccgccggga catccgggcg gagggctcgg
     6661 gcaacgccgg cggcacgaac accttccggg tcttcgggtg gaaggccggt gtcctggtgg
     6721 tcctggtcga cgtgggcaag ggagtcgcgg cggccctcct ggcctcccgc ctggccgcgg
     6781 gcacccccct gcccgaggac gccgtccgcc tggcctgcgg taccgcggcc acggtcggcc
     6841 acgtgtggac ggtcttcgcg ggcttccgag gcggaaaggg agtggccacc gcggcgggca
     6901 ccctggccgc cgtcgctccc ctgccctttc tggcggccct ggccgccttc gccctgggga
     6961 tcctgtccac gggtatagtg tccgtgggct ccctggccgc ggccgtgacc tttcccgcgg
     7021 cggccctggc cattcgagcg gccgggttcc cggtctcgaa cgtccttctg ggcttctcct
     7081 tcctgctggc gcccctgatc atctggacac accggaagaa tataggacgg ctgctccggg
     7141 gcgaggagaa ccgcttcgag aaactgcgcg tcctgcccag gctattcaga aaacaagact
     7201 taaaataata gatccaacaa gatctacaag atcaattaag atgaacaaga tgaatccaat
     7261 ttagggaaaa gtgttctagg ttggatagga ctatcgaact ttcccatctt gtgtatcttt
     7321 acttttcttg ttcatcttgt tggatcgatt aaaagtagcc ccgccctcat ttccccttga
     7381 ccgcgaacgt cctctccagg agttgggtgt tccaggtgtc cttgacaata agcttgtact
     7441 gcccgggctc ggcgaagaga taggccggga gctccaggtg gccccccgcg gggatgcggg
     7501 ccgtttcccg gtggatttcc tgggattccc ggaactgcgg atcggccttg tagaatatca
     7561 cgacgaaggc tccgtccttc ccggtcccgt tcgtcagggt gaaggagatc cgggtccccg
     7621 cggccgcggg gatgggatcc gcgtaggaag ccctctcccg ggggatggtg aagccgatgt
     7681 cggcgacggg gggttcctcc cgggacgggg ccgccggggc cgcggccgga gcggcggcag
     7741 ctggtgcgga cgcggctgcg ggcggccgga ccagggcacc ggccgagccg ggggaaccca
     7801 ggaaggaccg ggccgcgatg agccggggcg ggaaatagga atccgaagcc ggagatacga
     7861 tcacccccgt gcgggggccc tgggagaccg catggatgac cgagccctcc cccaggtaga
     7921 tggccacatg gctgggatat cctcccacgg tgtcatagac caggacatcc ccgggccggg
     7981 ccttgcgggg atccaccgga atccccgccg cgaagatccc ccgggaggac cgggggagct
     8041 cgatccccgc ggcctgccgg tatacatacc ggacgaagcc ggagcagtcg aaggcagccg
     8101 gggactcggc tccgtacatg taagggaccc cgcggaagcg ggacgcggcc tccaggatcc
     8161 gtctccggac ctcatcctcc gatacctggc cgaaggatcc ggccgcgacc gcgaagagga
     8221 ggatcccgat gcccaggttc gcccggccgg ctttcaataa attcccgtaa gcgccttgat
     8281 gtactggagc cgctcgtagt ccgcggggct ccggctggtg aagcgcgaaa gcttgccccg
     8341 gatgtcatcc aggccggcgg ccttcctctg gtccagccag gtttccatcc ccgtcaggat
     8401 ctcgcggatg accccgtatc ccttctggta gaccaccgag cagacctgca cggcccgggc
     8461 accggccagg atgaccttga gcgcggacgc gtagtcgtgc acccccgtgc ccgcggccag
     8521 gtcgcagggg acccgcccga agagcaggga gatccaccgg aggctctcgt ggtagtcctc
     8581 gggcttgctg tgcaccggtc ccgccatgag gcccagggtg gaaaggtcta tatccatgcg
     8641 gaagaatcgg ttgaacagga ccagcccgcc agcccccgcg gagctgagcc gggaggccag
     8701 gttcaggagg ttcgtgtact gggaaccgag cttgacggac accggcaggc ccgacgcggc
     8761 ccgggcggtc cggacgatct ggactacccg gtcctccagg ctggaggccg tctcgtccgg
     8821 ggaggtggac aggaaggcga tgttgagctc cagggcgtcc gccccggact tgccgacctg
     8881 ctccgcgaac tcggtccacc agggtccgcc tatgcagttg acgctggcga tcacggggat
     8941 gtccgccgcg gccttggcct cccggatcag cttgaggtac tccccggaac ccgcatgcat
     9001 gcccatctcc gccaggtagg cgtccgcctc gggatgggat tcacccgcgg aacctcccac
     9061 ggactggatg tccgcccgga gctgctcctc gaacaggctc ttcaggacca cggcccccgc
     9121 gcccgcgtcc gcggctttct tcacgcccgt cggagtcgcc gtcaggctgg atgccgccac
     9181 cacgacagga ttcttcaggg taagcccgag gtacttggtc ttcatgtcgg ccatgttcgc
     9241 tccttgttcc gtgtctctcg cctctgctcg tcccgcatcc ggcctcgaat accggaaaaa
     9301 ccgggccgga gggtccgcag ttcgtaatat atcaagcggg tcccgatgaa attcaaacgc
     9361 ccctcctcag ggcatccgtc catccgaacc gggacccgct tccaggatca agaaaataat
     9421 atatgcatcc tttcgtaaaa tacaagctcg ccctggggcc cagggtgtcc gatacgtcgg
     9481 ttactttttg aacagctccg cgtcgatgat acggcgggag aggcttcccg ggaggggaag
     9541 gggcggatgg tcgtccggcc cgtaccagcc cgcctctgcg atctcgatcc cgtcgggggc
     9601 aagctcgccg gattcccact ccgcctcgaa gccgaccatc agggaatccg gaaagggcca
     9661 gggctgggat cccaggtacc ggacgttccg gacccggatg cccacctcct ccgcgacctc
     9721 ccggaccacg cagtcctcga agctctcgcc gggctccacg aaccccgcca ggacactgaa
     9781 gatcccggtc ttgaaggcgg cgttccgggc caggaggatg cggccgtccc ggtggacccg
     9841 ggccaggacc gccggcgaaa ggcgcgggta ggtcaccgaa ccgcaggagg gacagagccg
     9901 ggcggtctcg tccggcttgt ccccctgggc cgagccgcag cgcccgcaga accgggagcc
     9961 ggccctccag ttggccaagg ccagggcccg gcaggccggc aggaccccgt cccaggcaag
    10021 gctccccagc accgaacgga agggtaccca ggacaggccc tccggagctt cggcctccgg
    10081 ggccagggag aggaccgtgg gtacgggacc ttccttcgcg ggatcggcgg ggcccgggat
    10141 ccgcagggga aagacgtcca ggagccaggg atccttgccg ggcttccggt ccggaaaggg
    10201 atgggagtcc aggggctccg cgcggccgtc gggacggcag agaagttcct gcccgcggaa
    10261 ggcgaagtaa gccggcttcg gctgggtgtc ctgcatggtg ttcctcgagg ccaggatata
    10321 cgaagaggcc gtcccggggc tacgccttcc gggggcttcc gccctggaat ttcagttctt
    10381 ccttgggatt ggactccgga tccgcctcgc ggcaaaccgc aggaccgcgt acattcccgc
    10441 agctccgagg gcggaggccg ccgcggccgg ctccgggacg gaaaggccca ggaaagtccg
    10501 ggcggccggg acgaggaagc ccgcggcggc cgggaccaag gccgcgagga aaacgcctag
    10561 ccggacggcg atcctccgcg cgcgggctcg gggattcccg gactccgggg gcgaccggag
    10621 ccttcccggg ggttccccgg cctggaccag gcagagggcc aggaagacca ggctccgggc
    10681 ccggtctgcc gcccggggcg ctaccccggc gtatcccagg cccaggcgat agaccgccag
    10741 gaggaggagg gcaagcagga gccacgggac caggatatcc gggatgatcc gggaaggacg
    10801 ttccccttcg gtcggcgccg taggccctgc gtccccgcgg acgaaggcca gggacagtgt
    10861 cggcaggacc gttccgacgg cgttggccca cagcagctgg gcggtcgaga gcgggggaag
    10921 ccagcccgcc agcacggagc cggccacggc caggctcatc cccgcgcagc aggtgaactg
    10981 gaaggcctcg acccgggaga acccatcccg accggtccgg ctccgaagga aggcggccgc
    11041 cgcgggcagt cccaggggca gcagcagaac cgtgcaggtc agggccgact ggatcgagtc
    11101 caggaaggcg gaccggatcg gaaagccccc acgaaggagg tccgaggcga ggaagaccgc
    11161 ctggaaggac acgaaggcga ttccggcagg caggaacggc cggaacgagg agaccccggg
    11221 gaccgggcgg cccgagcccg cggccgggcg gcccgagccc ttggccgggc cggacgtcgg
    11281 ggcgccggac gaatcggcga ccggtgcctg ccgggcggct ccgcgggacc acgcctcccc
    11341 ggccagggac agagcccagg ccgcggccgc cgtcagggcc gccccgggct cgcccaggaa
    11401 ggcgtaggca aggacggccg cgggaaggag ggcgcggacc gaggcggctg ccccggctcc
    11461 cccgcccgcc gtgaaggcgc gcgctgcggc ggggttcgtt ccgcggctcc gggagcttcg
    11521 agtcggcatg cgcgccttat tccgacagcc ccgccgcctt gaggagctcc gccggcttgg
    11581 cgcgctccgc ctccggaagg gccgccagca gggccgccgc ggccttggaa tccgagaact
    11641 tggcgtctag cgccttccgc agcgcctcca taccggcctc ccgatccccg gccaccacca
    11701 gccgaattcg ggccagccgg aaccaggctt ccccgtccgc cgggtccgcc tgggccaggg
    11761 ccgcccagtc ctcctgggcc cgggcgaata gctcccgggc ttcccgaagc tccgcgcggg
    11821 cgcgcaggac ttcccggtcc ccgggcttcc gggacaggta ggccgagagc agggtcaggg
    11881 cgtcctcctc ggcagaagcc gcggccgcgg ccgtggaagt gatcgcgggc gcggactcct
    11941 cccccggggc gggggattcc ccggagcccc gggtcccgga gacgcggcgg gcctccagca
    12001 gggccagccg acggaggact ccgtcgtcgg cggggttgac ggcatacaac tcccgcagga
    12061 tcaccaaggc ctccccgtcc cggcccgccc gttccaggag cagggccagg ttccggaggg
    12121 tgcccgcgtc ccgggggttc agctccgccg cccgccggta cgcggccagg gcggcctcgt
    12181 cgtccccgga cttgttaagc acataggccc tgaaagcgat gaggcgcacg ttgtcggggt
    12241 cgtcggagcg cagccgctcc aggacctcca gggcccgggg ataggcgccc gcctccgcca
    12301 gggcccgggc caggttgtag gaggccgcga ccaggctccg gtcgtaggcc agggccctct
    12361 cgtaggcggc cccggccttg tcccacttct ccatggccag ccaagcgttc ccgatctcgt
    12421 agtattccag ggcgatccgg ggcgcctggg cggtggcgca ggaggaaagg agggcggcca
    12481 aagccgcggc cgcggcgagc acggcgcgta cggccgcccg ggccccgccc ttccctatcg
    12541 ccgtagcacc atctcctcga tcgtccggat ctgggagcgg tagaggttcg cgatgttcga
    12601 gccgtaatcc gcgatcagct ggagcatatc ccggggccgc tcgtgctcgt ccaccccgtt
    12661 cacccggtcc cccgcgtccc ccaggccggg catgatgtag gccatctcgt tcaggaccgg
    12721 gtccatccag agggtgtaga ccgtggcctc ggggatggcc cgcacgaccc tcagggcgcc
    12781 cttcagggcg gagatcacgt tgaagacctt gatgctccgg ggcttcaccc ctaggtccag
    12841 gaggtacttc acgaccgtca ccaggctccc ccccgtggcg ttcatggggt ccgcgaacaa
    12901 caggtcctta ccgtccatgt cctcggtgcg gaaataggag cggtccaggt ccaggacgta
    12961 ctccatgttg ttctccacct tggtgtcgtc ccggctgatc ttgaacaggg cgaagggggt
    13021 cacgtatccg gaggaggagt attcctggat ctccttggac aggatcatgg agggcaggag
    13081 ggcccctctc agcatgacgc acatcacggt gttttcgatc cggtcgtcga tattcgggat
    13141 cttgtggacc gcgtagttct ggaccggaac cgtgaccggc gtggacacga tcaggtggtt
    13201 cttgttctcg ctccgggcgc cgccgaaggc gaggttgaac aagagctcgt aggcccgctg
    13261 gatgtagtag acgaactcga agcgctgggt ccgggcgtcc cggagtttgg cgatcagccg
    13321 gctggcctcc ccgtggttct ccgagggggt cacgaaggaa tagacctggg cccggggttc
    13381 agccttgcag agttcctgca tcgccccgcc catccggttg tagaggtcga tgaggacctt
    13441 ctcctcccgc cggcgctcgt ccgcgccctt ggcgtgggag aggatgttga agctcaccat
    13501 ggcctgccgg tagaggacgt ccagctggcc caggaatttc tggtccccct cggtcaggta
    13561 atcgtccagg tcctgggcct tgaggatgac gcggttcata ggaccacttc gccttgggct
    13621 ccgttcccga tccccacggc gttgccctcg tcgaagtcca ggtggcactc cagggcgtag
    13681 gcggcgttca cccggcacac cacgttgtcg aaggtggccc cccggtcccc gccggcgtgg
    13741 agcttgacga tttgcttgtc cttgaccccg aaacgctcgg cgctggccgg atcgaagtgg
    13801 atgtgccgct tggcgatgat gagtccctgg gtcttcctca cggagcccgc cggtccgacg
    13861 atctcgaagg cggcgctccc gtccagctgg ccggactccc ggcagggcag ctcccggaga
    13921 cccaggcgga aggcgtcgct ggccatgacc tcgcactggg tttccttgcg ttcgggcccc
    13981 aggatgcgga caccctcgaa ggaacccttg ggtcccttga tggtcaccgt ttcctcgcag
    14041 gcgaactgcc cgggctgtag gaggtccttc atcttggtca gctggtggcc cttgccgaag
    14101 aggatttcga cgtcctcccg gcacatgtgg acgtggcggt tggacaggtt gatgagcacc
    14161 ttgttctcgg gcatgggagt cctcctggaa cgcggccccg gcctcggccg cggcgtacgt
    14221 tgatgattgt agtgtcgttc cagagtaaac gcgaacggat cggaggtcaa gccgattcgg
    14281 ggcgcctacc gctcccggag ctccctgccc aggagctccg cgacggcccc cgcctggttc
    14341 cggaccgctt ccgcccgtcg gggcgggtcc gcccgcacgg ccaggtcccg ccgtccgtcc
    14401 aggacgaggc cccaggctgc ccagcccctg ccccccttgc ccaccagccg aggatacagg
    14461 gagcggaggt cgtccnnnnn nnnnnaaatc ctccgcggtg gggcggtcca cggtctccac
    14521 gaacacggcc tcgacctcct cccagtccag gtcgaaggac cggggaaagg gcaggatccc
    14581 gaagcgcctc cgaatgatcc gccgcgcgga atccaggatc cagctgtcct cggagagggc
    14641 ggccagcagg cacaccgcgg tcccggccag ccagaagggg tcgccggatc ccagggagcc
    14701 cagggcgaag gccgccgcga aggcggcctg ggccgcacgg accagggggt gcacggccag
    14761 gtcgacgcgg cccggggcct tcgggcgcag gaccagggga ggcgagaagg ggctcacggc
    14821 cgttcccgcc cggcaagctc ctcggccgcc cgggatgttc cgaggacggc gagtcctttg
    14881 tcgaaggcgt cccggagggc ttcgtccccc cgggacagga ggtagaccac cgagagttcg
    14941 tggcccgtct cgaaacaggc ctttacggtc ccggaaaaat aattctccga caggtaggcg
    15001 cccaccaggc gctcggtgat cgcgcagtcc gcccgctccc agatgaccgc agcgaaggct
    15061 tccaggtcgt tcgcggtggt cacgacctcg cagcctgtcc cccgcaggca gtccaccacg
    15121 taggagccct cctgggccgc cacccgccgg cccgagaggt ccggaaggcc ccggaacgag
    15181 gctgtccggg atgtgtgggc gaagaccgcg ggccggaaaa ccccgaaggg ccgggtcggg
    15241 atcaccggct tgtccgccag gccctggttg ggccaaccca cgttggcgac cacccggacc
    15301 ttgccggcca ggaggtccga cagggcctcg aagaactggc cggggtggaa ctccggaacc
    15361 aggcccgccg cctccagggc ccttcgggcc gtgtcgatcg ccttgcccgc ggactggccg
    15421 ccctggatgt ggacccacgg cggatagctt acgtcgtggg ctatcgggat cgtctcgccg
    15481 caggcccggg agccgaagag gtcggcgaag gaagtggcca tgtggttgat ctcggtcttg
    15541 accgcgcccg cggcggactg cacggcctcg gaagaggctt tctgccgttc caggttcccc
    15601 cgaaccaggg cggagttgtc gttcaggagg cgggccgcct cggacagggc cgtcatggat
    15661 cgggcgatgc gtccggagct ctccttctgc ccctccaggg cccctcggat cgagcgcatg
    15721 ttggaggcag ccccggcgac cgcggaggcc ccggagccca actcgtcccg ggcgtgggcc
    15781 atgcggcggg acagctcggc cttgccctgg gccatggcgg caagaccctc gtccgcggag
    15841 cccatgcccg cccggaggcc cttgagcagg cccgctgcgg tgtccgtcag ggcccggctc
    15901 ttggccgcca gggccttgac ctccgtggcc accaccttga agcctcggcc gtggactccc
    15961 gcccgggcgg cctcgatcgc ggcgttcagg gccaggaggt tggtcagctc cgcgatctcc
    16021 tcgatggact ggagggcctc ctcgatgcgg tccgcgctct cggcgacccg gcggaacata
    16081 gatatgaaga gctcgaaccg ggaatccata tccgccaggg ccttggtccc gtcctccacg
    16141 gctccccggg cgctgcccac cgaggactcc gcggcctcca gccgggcatc cgcctccccc
    16201 gccgccccgg agatgccgga gatcccgccg ttcagggact ccaggtccgc ctcgatcgcc
    16261 cgtagggaat ccaggttctt ggagttcgcg gccaggctct cctcgaaaaa gtccgtggtg
    16321 aggtccgact cgatggacag ggaccagacg tggtccgcca gcacccggta ccgggagcgg
    16381 agttccttga ggagcgcggc cgcctgggcc tctaggggct gccctctgga ttcctgggac
    16441 atgccgatac ctccgcgggt atgggggaca gagctcgtgg attccggact gcggcgcaat
    16501 ccggaaccgc ctacgattat tccatggttt tgcggtatag gctagggctg aattgacatc
    16561 ctggcggtat tcgtcaagct cgaggtccgc cctaaacgga gccgaaacgt ccgtccgaca
    16621 ggagggacgc caagtcgtag aggctggcga tccggagact gtcgtagtcc ggatgccggt
    16681 tccggtcgtc caggaggacc ccgttgcccc cgcactccag gtagccccgg acgtaccggg
    16741 ggaggtcgtc caccagcagg gtttccgacg gcttgacccc caatgaggag cagacccgga
    16801 ggcccgcggc cggatgcggc ttgcccttga aatggttgaa gcggatgtcg aagaccgcct
    16861 ggaagacgtc cgggaagccg agccgcccga ggacccggtc cgcgtgctcc cgggtggaat
    16921 tcgtgaacac gaccttggga tacggcaggg aacgcaggaa gtcccccaag gaggggtccg
    16981 ggtcgatgca ctcctcttcg ccctcgggat ggacggcggc caggtaatcc tcgggatcgt
    17041 cgaacccgta ctcggccatg agccactcca gggtggttcc gtgccggggc atatcggccc
    17101 gtctacggtc cagggcttcc tccacggaga ggttcaggaa ccgggcggta tactcgttca
    17161 tgcgccccac gatctgggac tccatggggt tggaggaggg atacagggtg ttgtcgagat
    17221 cgaagagcag acaccgtatc ataggaagga ttcataccat gcaaccgggc gcgtgaacaa
    17281 cccaagcttg acccgacgcc ggtttcgggg gatgatgggc cgccagggag gaccgggatg
    17341 cagcgcatac tttatctgta cctgaacacc ggcggcggcc acatctccgg cgcccgggcc
    17401 ctcaaggcgg ggatggagaa cctctttccc ggccgggccg agggccatct cctgcacggc
    17461 tacccgcccc ggctggtcct ggaccgcctg gtgatggagc agggctattc cctggcgatc
    17521 aaccacttca tgcccggcta ccttttggtc tacaagctcg cggagctccg tcccctcatc
    17581 accctgtccc aggagaccac ggcccaggcc tgcgcgggct atatcgcctc ccacatccga
    17641 aggctgggga tcaccaaggt cgtctgcctg cacttcatcc tggagaaggc cgcccgggac
    17701 gcgatccggc aggtggaccc cgccatgccc ctggtcacgg tggtcatgga ccccttctcg
    17761 gtccccccca tctggttcgg agtgaaggac caggagttct gcgtcttctc cgagcgggcc
    17821 cgcaggtccg ccctggacga gggcgtcgct ccggagcggg tccgggtctt ccccctgatg
    17881 ctccggaagg agttcgagcg tccccggccc ccgggggaga tcccggagct gaagaggcgg
    17941 tacggcttcg accccgaccg ccccctcctg ctcctggccg ggggcgggga cgggctgccc
    18001 cgctcggacc gtatcgtccg ggaggccctg aaggacggat tcgaggggca gatcgccgtg
    18061 gtctgcggca agaacatgct cctgcggcgc cggctccggg acctgcgctc ccggctgggc
    18121 gctcgggacc taaaaatcta cggcttcgtg gacttcatgg cggacctcat ggccatggcg
    18181 gactgcgtgg tcaccaaggc ggggcccgcg acggtcatgg agaccctggc cctccgcaag
    18241 cccctgatcc tctcctgcta cgtccggaac caggaaaaag gcaacgtgca gttcgtcacc
    18301 tcgaacaagg tgggatggta cctgacggag ccctccgaca tcgtgggcaa gacccgggag
    18361 atcttcggca agcctgaact acgcagggag ttggaggggc ggatcgcggg gctcgggatc
    18421 cggaacggcc tttccgacct ggcccggcac atcttcgagc tgtgatcgga cttccccttt
    18481 gaacgaagaa tcgttatgtt ggaatcgtcc gaattccata gaaatcctca tacaccggag
    18541 caatctatga accgatcgaa attctgtatc ccggccctct tcatcctggc cctagcctcc
    18601 gcgcccgcct tcggggcccc ggagaagaag ctggacgtgg acctgtctcc ctccaactgg
    18661 gccctgggga ttttcctggg ggagcccacg ggcatcaccg tccagctcga cgtgggggcg
    18721 acccaggccc tggaggtcaa ggccgcctgg aacttcgtgg ccgccggcca gaacgagggg
    18781 ggcaacgtca ccctccaggg gaactacgtc ctctggttcc cggggctcct ggtcttcgac
    18841 ggccacgaca tccccccctt cgcgggaatc ggcgtcgaag cccggttcgg gaccgacttc
    18901 ctcctggggc tccatatccc cttcggagtg cggtaccggt tccgggaagt cccgatcgag
    18961 ctggccctgg agctgggggc gggcatgtcc ctgatcccct cgacgagctt catggggtcc
    19021 ggaggtctgg ccatccgctg gatgctgtag gggcgggtcc gggcgatgat tcccgtaggg
    19081 gacgacaact cccgcaaccg ccggacaccg gtcgtgacac ggctcctcct ggccttgaac
    19141 atagcggcct tcatcctgct ccagcagtgc gggaccgacc cgaacatccc ccggtccctg
    19201 ggagccgtgc cccaggaatt gatccagggc cgggacatcc ggaccgcgga ccaggtgatc
    19261 cgttccccga cgacgggccg gtcctatcgg gtgccgggcc tgctggaaac cccggtctgg
    19321 atctacctta cggtgctgac cgccatgttc ctgcacggga gcacgggaca catcctgggc
    19381 aacctgctgt acctggggat cttcggggac aacgtcgaga accggatggg accgatgagg
    19441 ttcctgctct tttacctgat catgggggcc gccgcgacgg cggcccaggt cctggcctcg
    19501 atggtcaccg gacaaggcct cctgaccccc atgatcgggg cttccggcgc gatctccggg
    19561 gtcatggggg cgtacctggt cctctttccc cgcaacaggg tctacatcct taccttcggc
    19621 ttctacccca tgcccgtgag cgcctggttc gccctgggta tctggttcct catgcagctg
    19681 gtcagcgcct ccgccgcggg tcccgcgggc agcggagtcg cctacgccgc gcacatcggc
    19741 ggcttcgcgg ccgggtggct gtgggcccgg atcttcgagg ccggggactc ccgccgcaga
    19801 ggacggggcg gccgggacgg ggacataacc tggaggatcg tggactaggg gggacgtgct
    19861 tccctcgcga aggataaacg gctatactgc ccgccggcta aacatggttc ggtcaagggt
    19921 cttggggcgg cgccgacggg cggccctgga cgccggatcg gcggcgcatc cgccgggacc
    19981 gaaaagcgag cgtgggcgca caggcgcgaa gcggcgaaac ggtatggcac aggacaaaaa
    20041 aacctcgggc aggaggaatc ctgtccggtt cttcacgtat ctttccccat attggaacct
    20101 tttcgtcgcg ggattcgcgg ccatgctggc ggtcacggcc gcccgtctcg ccggtccgct
    20161 catcctgcga gaactcgtgg acaaggccat tcctgcacgg gataccggcc tcatgctgcg
    20221 ctacgcccta gcctacatgg gcatcatcgt ggtcatgggc gcgctcacct acttccagac
    20281 catgctcatc gtgcgcctgg gactcaaggt ggtgacccgg atcaaggacg aactcttttc
    20341 gcatctcctg accctgcccg tgtcctactt cgacaagcat ccggtgggcg agctgatggc
    20401 ccgggtggag aacgacacgg aaaaggtgaa gcagctcttc agcgagaccg tcatcttcct
    20461 ggccaccaac gtgctctact tcctggggat gttcgccgtg ttcttccgca tggacgcacg
    20521 ggtggcttcc ttcatcctcc tgcccgtgcc cctcttcctg gccgccttcc tcttcctgtt
    20581 cggccgcctg cgccccctct acgagaaggc ccgaaaaaag tacgccgaga tcaccgcggt
    20641 ggcgacggag ttcgtccagg gaatcgagat cctccgggcc ttcaaccgca cggggtatgc
    20701 cgcggagagg ctcgacgccg ccagcaagga gaagcgggat gtggagatcc gggccggcct
    20761 cttcgagtac ggattcacgg gagggatggg gttcctggcg gggcccgtgt tcgtcgtcct
    20821 catcatccgg ctcgtggccc cgaacatctt ccggggagcc ctcaccgtgg ggacgctgct
    20881 cgtgttcatc gagtacggga tgcggctctt cgagcccctc ctggccatcg gcgagaacat
    20941 ccgcggcatc cagcaggccc gggtcgccct gggccgcatc ttcgggatcc tcgacctcga
    21001 acccgaggag tccccggacg cggggggcga tccgccgagc ttccggaagt ccatcacctt
    21061 cgaccgggtg agcttcgagt acaaggaggg cgagccggtc ctcgactcgg tgtcgttcac
    21121 cgtggagaag ggacagaccg tggccctggt aggcgcctcc ggctcgggca agaccaccac
    21181 ggtgagcctc ctgtgccgct tctaccccta ctcccgggga cggatcctcg tggacggccg
    21241 gcccctcgaa tccctggacc tccgggcctg gaggcggatg atcggcctcg tgctccagga
    21301 catctacctc ttcccgggca cggtcctgga aaacgtgcgg gtgtacgacg acacggtgac
    21361 agagctcgac gtgttcggcg ccttgaagac cgtgcacgcc gcggaattcg tccgccgcct
    21421 gccgaacgag ctccgcacgg aactgcacga acggggcgcc aacctttcca tgggggaaaa
    21481 acagctcctg tccttcgccc gtgccgtggc cttcgacgcc gagatcgtca tcatggacga
    21541 ggccacggcc agcgtggacg tggcgaccga gcggaggatc caggagagca tggccgagct
    21601 cctggcgggc cgtacggcca tcatcgtggc ccaccggctt tcgtccatcc tcaaggccga
    21661 caagatcctc ttcttcaagg acggccgcat cgtggcccag ggccggcacg aggagctcct
    21721 ggccagcctg cccgactacg gggacctggt gcgcctgcag ttccccgacc tcgaaggcgg
    21781 aggccgcggc gcggagaaac ggatcgcggc ggtggagact tcggcatgag cgcccgcggg
    21841 accgtcgacc acgtggcctg gatccgtgcc gtgtggagcg agcacaaggg cctggtggcc
    21901 ctcctcttct tcctgaccct attgtccagt gccgtggccg tgctctaccc ctacttgtcc
    21961 aagctgctcc tggacaccat ccagggcctg ctcgcctcgc ccggccttgg gaatcccatg
    22021 gacgaggtcg accgcctcct cctcgtgttc ctggccgtgg ggcttgcggg actggtggcc
    22081 tcgatgttcc ccggcatccg gggcatgacc aactccgtgt tcgagcacgt catccgaagc
    22141 aagtacttcc ggaaggtcct ggccaaggat tacagcttct tcgccaagtt ctccaccggg
    22201 gacgtggtaa cccggctcac cgacgacctc tacgacttcc ccaagctgtc gtggttcctc
    22261 tgctccggaa tcttccgggc ggtggagtcg atcagcaagg tggccttctg cctggccgcc
    22321 atggtcctct tgaatcccaa actcacctta tactccctcg tccccctgcc cctgatgatc
    22381 gcgatcttct acgtcaccca ggaccgcatc tacgacacct tccagaagaa ccaggaggcc
    22441 atcagcgcgg tgaacgcccg gctggagatg agcttctccg gggtgcgcat catcaaggcc
    22501 tacgcctgcg aggacaagta ccgccgtttc ttccggaacg tcctggagca gcgcttcggc
    22561 accgagctag cggtggccaa gctcgaggtc gtcctgcacc tcatctacca gtacatcgac
    22621 tacgtagccc aggtgggcat cgtgttcgcc ggcggcctca tggcggtgcg aggagagatc
    22681 agcatcggca ccttctacgc cttctacacc tacctgaccc tgctgatctt ccccatcctg
    22741 gacattcccc agctcttcgt atccggcaag cgcgccttcg tcaacatcga ccggctggag
    22801 gagatcgccc gccatcccga gcccgctgct ccggggaaac cgcgtgcggt cgaccggctc
    22861 gagcgcgtgg aattgtacgg cgtgtccttc cgttatgggg acagagctga gaactctctt
    22921 acggacatct gcttctctat cggccgaggg gagcggatcg gcgtcatcgg gcccgtgggg
    22981 agcgggaaat ccaccctggt caagtgtctc ctcggactcc tgccaccaag ttccggagag
    23041 atccgggtga acggtgagga cctccgggaa ctcgaccttg tctcctatcg cgcccgggtg
    23101 ggctatgtcc cccaggaggc cttactgttc tccggatccc tgcgagagaa cgtcgatttc
    23161 ggcggcgagt cgctttcgga cgagatgttc cggacctccg tggaagcggc ccagatggcc
    23221 gaagagatcg cctccttcac cgacggagag cgcaccgtgg tgggccagcg cggcgtgagt
    23281 ctgtcggggg ggcagaaaca acgcatggct atcgcccgag ccgtggcccg gaggcccgag
    23341 ctcatgctct tcgacgacat caccgccagc ctggacgcgg ccaacgagga acgcctcatg
    23401 cgccgactcg atgagctttc cggggacttg tccttcgtca tcgtctccca ccgcctgtct
    23461 accctgcagt acgtggacaa ggtcctgttc ttggaccagg gccgggccct cggattcggc
    23521 cggcatgaag agctgctgca aaatcgccgg taccgggcct tcatcgagga gcacctggga
    23581 aagtcagagt aagtattttt cttagggaca gagctcgaag caagcaaatc gggaaaccgg
    23641 ccgcgtatac gcggtatgcg caagccaaga gaattgaaaa ccggcgccct ctaccacgtg
    23701 acggcccggg ccaatcggca ggagatgatt ctcgacagcc gagggatgaa ggaacttttc
    23761 atgaaaaccc tctcgagggc caagaaaaag tacagattcc gggtcgagaa tttctgcgtc
    23821 atgggaaacc atttccatct cataatccgc ccggaggcgg gagaatctct ttccaggatc
    23881 ctgcaatgga tactgagcgt tttcgcgatg gcgtataaca ggatccatgg gctctggggt
    23941 catgtgtggg gatcccgatt cttctcccgg atcctggccg gcctgcggga atacctggcc
    24001 gtcttcgact acatcgatca aaatcctctg gcagttcatc gggtgcggga tccgcgggaa
    24061 tggatgtacg gcggtctttg ggagcaccgg cacggatacc gtacggtggc ggatcgtccg
    24121 gaaccgtatg tcgagatgat ggttccggat cacgggttgg tccgactgga acgggtaact
    24181 ccgcggaggc acgcccattt ttcttgaccc aagccggtct ctccgctaat ctgccatttc
    24241 cactatacgg gtggtatgga acgccatgaa tcaggggaag gctcggccgg ggaaatgatt
    24301 ttcccccgaa ataagtcgaa ttctcgtgta acctgcaaac catattgaag gatactttac
    24361 atggaacgat tgcagacact gctcgacacc ctcgtatccc atcaacctat caaacaagcc
    24421 attatggccg tggagagcgg cgacggctcc ttccgctggt ccggcgcggc aggaaaatcg
    24481 gttgacggat tgccgctcga tcccgaagcc cccttcttca tcgccagcat cgacaagctc
    24541 tacaatgccg ccgtcgtgct gatgctggcc gagcggggca aactcagatt ggacgatccg
    24601 atctccgact atctgcccgt cgatctgacg cggggattgc acactctgaa tggaatcgat
    24661 tattccggat cactcaccat ccggcacctg ctgtcacact cctccgggct ggctgattgg
    24721 ctggaagata cgccgaaaaa cggtaaaaac ctgatcgagc acgtgctgga acgagacgac
    24781 cgttcgatga gcactgccga gatcgcggac ctggtgcgcg ggttgagccc gcatttccct
    24841 ccccaggatc cgtccgctcg acgcgtcaag atacgctatt cggatactaa cttcatcttg
    24901 atcatcgccc tcctcgagac gatgtgcgga caacccctcc atcgtattca cgaggacctg
    24961 ctctacgcgc cgctcgggct tcgccatacc tatttccccg gaactaccct gccgcttgaa
    25021 acgacatccg cgccggttcc ccttcgtgcc gaaggacgta tcatcgaaat tccccggctg
    25081 atgacggcct tccgcggaat ctatagtact gcgggcgata cgatctcatt cctgcgccat
    25141 ctagcctgcg gcgaggtctt ccaaaacccc aaaactttcg cccttatgca atcccgctgg
    25201 aaccgcttcg gtttcccgat ggatcgggcc gccctgcgct cccccggctg gccgatcgag
    25261 tatggtctgg ggctgatgcg tttccagctg ccacgggcct tcacctcgct gaagcgcctc
    25321 ccggcggtca tcggccatac cggctcgacc ggctgctggc ttttctggtg ccccgaaatg
    25381 gatttgtatt tttccggcag cgtcgacgag gcgggtgccg gggccgtacc gtatcggatc
    25441 attccgcaaa tattgcagat tttggcgacg ggcgaaggag gtccgataca tggaatacgc
    25501 aaagtttagc ctgctgttca tgctggttca cacgggtgcg tattttattg ccggtatgct
    25561 tgcctatcgc gtcagtcgcg acctttacat gggcgagaat cgcctgctcg atttcgtgaa
    25621 agacatgtcc gaccagggcg aaaatgccag cgtggcgaaa ttgacccttc cgcttcaact
    25681 cgtgcgcggg ctggttcttt cgatcgtact gtaccccatc atcggattcc tcggagagtt
    25741 gtcgtatttg catcgttttc ttttcctcac cggcctgatg ttcgtttaca ctgactttgc
    25801 cagcgcaatc ccctttcccc ataatcttga aggactgatc tatttgaaag ctcggtactt
    25861 caagaaattc gcgttttgga aactgcaatt tgagatgatc gtcttcagcc tgatcttcgg
    25921 gctggcctcg ggttggattc tattccaatg agaccgcacg aacaggaaaa cacgtacatc
    25981 gacggatggg agaaatacca tgcagagatc gctcacatgg atcgttccgc tagtagccgt
    26041 actggccctt gccgcttctg cgaccggcct tttcctgccc ggcggggatg gcccctataa
    26101 atttacaacc ctgcgcggac aagaagtcga gatgttcggg cgcggattgt accgatacga
    26161 cacactcctg gtcggcgctg ccctgcgcgg caccgacgcc gtcactctgc tcatcggctt
    26221 gcccctgctg gtactgacct acatgaaatc ccgacacggt tcaaccaacg caccgctcgc
    26281 cttgctgggc gtcctgttct atttcctata caagggcgct tcgctgacct tctcggcggc
    26341 gttcaatccg ctcttcctgg tatataccgc cctcttttct gccagcctgt tcgccgtcat
    26401 catctccatg accgccttcg acctggagtc cttggcgaaa cgagtccagc ccggattccc
    26461 gcatcgcggc gcggcggtat ttctgttcat cgcaggaatc ggcaccctgc tgatttggct
    26521 gagcgaaatc ttcgtccccc tgctggcagg gaccgctccc gagatcatgg gaccctatac
    26581 cacactgttc acccacgggt tcgattcggc ggtcatcacc ccggcggcgg tgatcgcggg
    26641 cgtctgcctg gttcggcgca aaccgctcgg gtatctgctg acgattccta ttctcatcct
    26701 atgtacgctg atcggcgcgg tggtcatcgg gcagtccgtc gctcagacac tggtcgggct
    26761 ggtctttccg ccgcaaatct atatcggcat gatcggcagc tggatcgtga tgggcgggtt
    26821 tgcaatcgga cttaccgcct cgtccttccg caatctggag ccccccagcg gggacagagt
    26881 tcgatcctga caggacgctc ccgtaccgcc gaaagcgctc tcactctccg atgatcttca
    26941 tgaggacccg cttgtcccgg cggccgtcga actcgccgta gaaaatctgc tcccaggtcc
    27001 cgaagtccag ccgcccatcc gtcacggcca cgaccgcctc gcgtcccatg atcgcacgct
    27061 tgaggtgggc gtccgcgttg tcctcccccg tgttatgacg gtagccgttc cggtcgtagg
    27121 gggcaatctt ctccaaccag gccaggaaat ccgagtgcag gccgcgctcg tcgtcgttga
    27181 tgaagacact ggcggtgatg tgcatcggat tcacaaggca caacccttcc cgtatcccgc
    27241 tctccctcaa cgcctcctcc acccggtcgg tgatacgaac gatggcgtac cgctccttgg
    27301 tatggatcat gaactccttc cgatagctct tcacggcctc ctcccctccg cgcggccccg
    27361 gtcgcccgcg ctggccttaa tcatgccacg cggaggggca gccaggaaag ccagacccgg
    27421 ggacagagct cgacagagga aactcgcgta ttgacaaccc cctccggcgg cggggatact
    27481 tgcctcccat gccgcacagg aacctctcct tcgaggccgt ggtcctccgc tgccgggaga
    27541 gcccgggagg cgaccggatc gtgaccttct tggccgcgaa ttccggcctc gtggaggcct
    27601 tcgtgttcgg aggtccgaag agcaggctcc gatccctcgc cgccccctac cacaccggta
    27661 ccgcctggat atacctggat cccgtgaagg acctgcggaa gctctcggat ttcgacgccc
    27721 gggagaccta ccccaccctg agggaaaacc tgcgcaagat ctgggcggcc agctactttg
    27781 cggagacggt cctgcgcacc gacgccgccg ggggggagtc ccgggaggcc ttcgaccttc
    27841 tgcggtcggg tctgtccgcc ttggacgcgg gcggggaggc ggatgtcgac tacgtctgcc
    27901 tccagtacgg gtggaggctc ctgggcctca tgggcctgcg tccggattcg gagacctgcg
    27961 gacagtgcgg cgcgtcgttc ccggagggcc ggagggcggc gttttcgcgc atccaggaaa
    28021 ctttcctctg cgaccagtgc gcccgggccg gagattcggg gaattccgtg gacgacctta
    28081 ccctgagcgg gggtgccctg cgctacctgg ccgcctcgga ggctctgccc ttccgggacg
    28141 ccctccgcgt ccgcccttcc ggggaggacc tgccgatcct caaggccctg gttttccacc
    28201 tggtccggtc ggccgccgag ggggagctgc gcaccttggc acggggggag ctcctgtgag
    28261 gtgcttcatc gccctggagc tgcccgacga ggccaaggta cggttggccg cggcggccgg
    28321 gatcctggcg ggtcgtttcc cgggagcccg gtgggtgagg ccggagtcct tccacctcac
    28381 cctggccttc ctgggggaga tcaaggggac agagctcgac tgcgcccggg acgccgtggt
    28441 ggccaccgcc ggctcggggg ctttcgacct ggccttcgca gggacagagc tgctgccggt
    28501 gcggggcccc gcccgggtcc tggccctctc ggcgggtgcc gggacccggg aatgcgtgga
    28561 aatccacggc cgggtgaatc gagctctgtc cctggcagca cgggccgcgg ggcttccgcc
    28621 cctcaacccg gaatggcccg acggacggcc cttccgggtc cacgtgaccc tggcccgggc
    28681 gggaggccga cccttccccc gggacccccg gggcctcggg gaagcctggg aggaaatacg
    28741 ggcgtgcacg caagcgccct gccggatttc ccggtgtata ctgtacaggt cggccccggg
    28801 gccggggggc gcccggtacg agcctgtgct ggccacggta ctataaaaga cggaaaggag
    28861 tcgcgatgcg cgcggtggac ctgatcatga agaagcggga cggcggggag ctgtcccgcg
    28921 aggagatagc cttcctcgtt tccggctacg tggacgggag cctcccggag taccaggtat
    28981 cggcctggct catgtccgtc ttcttccggg gcatgaaccc ccgggagacc gcggacctca
    29041 cggagctcat gctccgttcc ggggacgtga tggacctctc cgggatcccc ggccccttcg
    29101 tggacaagca ctccacaggg ggtgtgggag acaaggtctc cctgatcctg gcgcccatcg
    29161 cggcggcctg cggggtcaaa gtgcccatga tgagcggccg agccctgggc cacacggggg
    29221 ggaccctgga caagctggag tccattcccg ggtaccggac gaacctctcc cccgcggagt
    29281 tccgggattt cctacgggcg gacggctacg ccatgaccgg ccaaaccgca cgggtggtcc
    29341 ccgcggacaa gaagctctac gccctccggg acgtgacggc cacggtggag tccgtgcccc
    29401 tcatcaccgc cagcatcctg tccaagaagg tcgccgaggg ggccgagtcc ctggtcttcg
    29461 acgtcaagtg cggccccggg gcctttatga agaccttccc ggacgcacgc cgcctggcgg
    29521 aaagcctggt ggacaccggc aaggccatgg gcaagcggat cgtcggggtg atcacggaca
    29581 tgaccgagcc cctgggcctt aaagtcggaa acttcctgga ggtcgaggag agcctggact
    29641 gcctgaccgg gaagggcccc gcggacctga tggaggtcac ctatcgcctg gccgcctgga
    29701 tgctcgtggc cgcgggcaag gccaagggcg tcgaggaggc cctgcggctc tgcgaggccg
    29761 ccgtggcgga gggccgggcg atggagctct tccgccgcaa cgtccggacc cagggcggag
    29821 acttggctaa gatggatgcc ctgtacggga agctccgctc gccccattcc ttcgagctcc
    29881 gggccccggc gtccggcttc gtcacggcca tcgacgccta caggatcgga ctcgcgggag
    29941 tctacctggg ggtgggccgg aacaccaccg cggatcccgt ttaccccgac gtgggcttcg
    30001 tcttcgagcg gaaggtcggc gactccgtga ccgcgggaga tctcgtggct cgggtgtatg
    30061 ggaaggacga agcgagtctg gccgcggccc ggcccctggt ggaggaggcc ctgaccgtcg
    30121 gacccgcagc ccccgccccc cggaaactcg tcctcgagga gctggccgcc ctatgaagtg
    30181 gaacaagaag gacgtgagcc cccaggcggt caaggatctg gccgcccgct tcgacctgga
    30241 cctcctttcc gcctccgtcc tggcccgccg gggcgtcacg gatcccgggg agctcctcta
    30301 cttcgtggag gacgaccccc gctacctcca caaccccttc ctcttcgacc agatggaaga
    30361 cgccgtggac cgggtcctcc tggcccggga cgagggggag aaggtcctcg tgttcgggga
    30421 ccgggacgcg gacggcatct ccagcaccgt cctggtctac gaggccctga aaagccgggg
    30481 cctggacgtc cggtatcgtc tgccctcggc ggacgatccc tacggactct cggccaaggc
    30541 cgtggaggag ttcgccgggg actacggcac cctgatcatc accgtggact gcggcatctc
    30601 gaaccacgcc gagatcgagc gcgcccggga gctcggcgtg gacgtcatcg tcctggacca
    30661 ccacctccac cgtcgggatg agctccccgg agccgtggcg gtcgtgaacc ccaagatcga
    30721 gtcctgcggc taccccttcc gggacctggc gggctgcgga gtggcctaca agttcacccg
    30781 ggccctggcc ttcgcggaga gcggcctcta taagcagcag gtggccctgc tcaacgtgcg
    30841 tccagccaac gacgcctaca ccctggaggc cgtgcgcctg acgaacctcg tggagaccgg
    30901 ccgcatttcc gagaccatcg tcccggggat ggtggagctc gagaagacac gcctggtgcc
    30961 cttcctcaaa gaccgccaga ttttcgtatg ggacggggat ttgcagaaga aactcctggc
    31021 caaggccttc ggacgggccg cggacgtaca ggtctacgac atcgccgagg acatcgggga
    31081 cctcatcccc gccgtccggg gcaagagcct cctgcgcctg cgggagcagt cccggatcgc
    31141 caagtactcg gacaaggcgg ccggggagat cgacgtcttc gccagcctct tcgtctcttt
    31201 cgccctcaag aagacttcgt acttcggcga ggcggacctg gaggcccttc agctggtagc
    31261 cgtcgggacc ctggcggacc tcatgcccct gcggaacgag aacaggatcc tggtgcgcaa
    31321 gggcatcgcc gccctcaacg ccaagccccg ggcgggactg gcggagctcc tggccaagct
    31381 gaacctcacc ggcaagcgcc tggccgccaa ggacctggcc tggcagctta cccccgccat
    31441 caacgccacc ggccggatgg gctccccgga gaccgcggtc cgcctcttcc tgggccagac
    31501 cgtggaggaa cgctcccgcc tggccgagga ggtcatcggc atgaaccaag atcgccgacg
    31561 cctgggagcc gagggctggg aggccgtcct gccggacgcc cggaagagcc tggaggccca
    31621 cgacaacaag ttcgtcctgg tcgggcggga cgatgtcaac cggggcatta ccgggatcat
    31681 cgccgcccgc ctctcgaaca ccctccgggt gcccgccatc gtcgccgcct ttctgggcgg
    31741 cggcaccatc gtgggctccc tgcgttccct ccggggctac cccgtcaagg ccctgctgga
    31801 ggcctgctcg gacctcttca tcgactacgg gggccacgac gaggccgccg gcttttccct
    31861 ccccgcggac aagtgggatg aactgacccg ccgggtcctg gaattcacca aggccgtgga
    31921 acttgaggcc gaggaggagt ccctgtccat cgacgcggag cttccccacg actacatgaa
    31981 gcccgaaatc acggacttgg tggaccgctt cgaaccctac ggggaagaga acggtcccct
    32041 ggtcttcctg acccgggacg ttcccatcgc ctcgatggaa ctggtgggca agaaggagca
    32101 gaaccacgtc aagatgctcc tggactacgg caagcatcgc tggcccgccc tcttctggtc
    32161 cgccgccgag cgggtggagc gggacttctc ccggaccgac cgggtggacg tggtcttcca
    32221 ggttggccgc aattactgga acggaatgga acaagcccag ctcgtggtca tggacgtacg
    32281 gcggactggg gaggacggga cccattgaca gaccgccccc gttttcggta ccttgtccgg
    32341 cgtcgacgca agtcgcggtc cccggttgtg taatggtagc acggcagact ctggatctgc
    32401 ttgtgggggt tcgaatcctc cctggggaat acggagtggc gcccttcgaa tcttgggacg
    32461 cgtctccggg gacgggcggg taccgatcga gactgtacgg atgggttcca tcgtcaggct
    32521 ctagggtatg ctggaaagta tgcagatttt gcctcgcaaa atctgcacgc gagcgaatcg
    32581 gtcttgaaaa gcggtagacg ggaattcgcc gagcgagctt tgtgagctcg gcgccgcgaa
    32641 gcgaaaaatc ttccctggaa gatttttcgc ttcgcgaggc cccttcgtct atcggttagg
    32701 acaaaagatt ctcaatcttt aaagaggggt tc
//
LOCUS       DAOTEI010000018        31394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_950_length_31394_cov_4.800249, whole genome shotgun sequence.
ACCESSION   DAOTEI010000018 DAOTEI010000000
VERSION     DAOTEI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 31394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31394
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_950_length_31394_cov_4.800249"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /gene="aspS"
                     /locus_tag="P5313_03005"
     CDS             complement(<1..1783)
                     /gene="aspS"
                     /locus_tag="P5313_03005"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRY79366.1"
                     /translation="MEYLKRTTTCGALRKGDAGQIVALNGWVHRKRDHGGISFINLRD
                     RYGITQVVVDADAEAALKASAAELKNEYCLSVRGRVRARPDSMVNPDMPTGEIEVVVS
                     ELQILTRCETLPFQIDERSDAKEETRLKYRYLDLRSGAMQYRMRLRHEVAFAVREHLA
                     SEGFYEIETPTFIRSTPEGARDYLVPSRLYPGKFYALPQSPQLYKQILMVSGMDKYFQ
                     IARCYRDEDARGDRQPEFTQIDIEMSFVKRDDVLSMVERLFGHVFRKTLGEELPANFR
                     RISYDESMELYGTDKPDLRFGLAMKDIGDLAARSTFQAFKDALAAGGAVKALVAPDKA
                     DYSRKQIEELEAAAKVYRAKGMAWFKVAAGEGGAAAQLEGGASKFFAGEGAPAGLASD
                     LVAALGAKSGDLVLMVADAKRKVACQSLGAVRSKLGKDLGLCDPKRFEFAWIVDFPMF
                     EWNEDENRWEAAHHMFTMPQERFHATMESDPGSVKGDLYDLVLNGYELASGSIRIHDP
                     DLQKRVFKIVGFSEEDAQKKFGFLTEAFKYGPPPHGGIAPGLDRLVMLMAGDTSIKEV
                     IAFPKNSLAVSPMDDCPNEVDQKQLDEL"
     gene            1883..2599
                     /locus_tag="P5313_03010"
     CDS             1883..2599
                     /locus_tag="P5313_03010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRY79367.1"
                     /translation="MRYLLVRHGPPRSPYNFEADLAASGARLDTVLLSEGDRPAAPAA
                     YDAVVVFGGYMYATEDDKYPWIRDELGYMESAIKAGVPLLGICLGGQLLARLLGARVY
                     KTPVPEFGYWPIERTEAGRSSVLFEGLPEPLLGFLWHNDAFDLPSGAVRLARTEHCPN
                     QAFSYGDRTFGLQFHLEFSGDQLRSMVTRDAASLPAPSPVRQDPAAVAANEAAHAEVK
                     SSMLRMLRTVGQLRETRRSP"
     gene            complement(2641..3897)
                     /gene="pepT"
                     /locus_tag="P5313_03015"
     CDS             complement(2641..3897)
                     /gene="pepT"
                     /locus_tag="P5313_03015"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRY79368.1"
                     /translation="MDNYKEALSDPFAGELAERLTRYARIETTSDRHVEDIPSTPTQW
                     DLARVLQKELQDLGFSDVTLDDHCYLSARLPATPGYEGKPSVGFMAHMDTASDVTAKD
                     AKPRVVPDYDGKALKLSDEHTLDPAAYPDLADHAGDTLIVTDGTTLLGADDKAGVAEI
                     LTALGHLIAHPEIAHGPVEVFFTPDEETGKGMNLFPLAKVRSVACYTLDGGKLGEIEA
                     ECFTAYSAKLTFKGYVIHIGSARGKLANATAMAASFISMLPRAESPESTDGWYGYYCP
                     IEVQGTLESASVEVFLRDFTDEGMNRRIETVKALAKAVEAQFPGGSVQADIKKQYVNM
                     RRKLDERPEVLERLVRACKDVGIPPVIKPIRGGTDGARLTEMGIPTPNIFTGGYNYHS
                     RYEWASLSEMVLTVRTVLRLIRLWAA"
     gene            3998..4870
                     /locus_tag="P5313_03020"
     CDS             3998..4870
                     /locus_tag="P5313_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRY79369.1"
                     /translation="MAIPEYPDFAPISLDMRDEMYPSLNLLSDGISEFTFSNLFLFRH
                     AYGYKVSRIPGQTLVLRGVKNGEPFFLTPCAVPDREHLEDLFKDHLYLKNLSESQSQV
                     HRVELEQWGYRIQEDRDNFDYLYHRTDLAELSGKAYHKKRNLVNAFLGTCACEQRPLT
                     KANVADAVEVLDRWREAKGVEGDYAASREALEYFEELGMRGAVYYADGRPAGYVLGEP
                     ISKARMFAVHFEKAVDGYKGIYQFINQAFAQTLPAYFRIVNREQDLGDEGLRQAKMTY
                     RPFGFVKKYRVYRA"
     gene            4896..5942
                     /locus_tag="P5313_03025"
     CDS             4896..5942
                     /locus_tag="P5313_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79370.1"
                     /translation="MTSSLVRLTGTDHDKVFGLRDTLLARALSENRRGIRSLPEYLEY
                     LAGRTVCLTLLAGAGSRWVDSLRQAAAEGRDLRFDPARPRGLFPVRNALGRGPDPVPI
                     AAYAVDAFRGLGSRVIVVRGWEAEIDREILEPLGVSPEERRFFTQEAPLGKPLGHGDA
                     VWQCRDLWKDAEYVLVNFGGDASSPFTARAALVVLDALVSLGEPVGLLLPAARFERPA
                     YPITLDGLGRPASFGHAKLSGTAVRSSSGWANVGLRAYKAPSLLALCETIRAEHWKDG
                     EGYSIPGNDPAGREFALDNVDARLAEGRLVRLLAAALPEELSPVKSLADVPAFEEAAA
                     RVRRDAAALPGGDP"
     gene            complement(5947..8013)
                     /locus_tag="P5313_03030"
     CDS             complement(5947..8013)
                     /locus_tag="P5313_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY79371.1"
                     /translation="MNNEKILIVEDEKIIALDLQRRLERFGYTVVGMASEGETAVELA
                     RQAGPDIVLMDIYLAGTMDGIDAAGIIRNQLGLPVIFLTAYTDERTLERAKEVEPYGY
                     ILKPLKERELYTTIDIALYKYGIEKRLRKQERLFSAILHSINDGIVATDMNLNIQFMN
                     TVAEEITGYREEAVQGACLTRILTLFDPRTQKDLLGSGAPPVEERPYFFHEVSIKNNL
                     NQTILVDGSITRIHEKENATEGYLLALRDITEMKRMSETINYQASHDILTGLSNREEL
                     SFKLGEVLKNIQQTGDSHGLLILDVDRFKAVNDTCGALAGDELLRQVASHIQANIQRN
                     DISARIGGDEFACILMNCAVQDAFNVAKRLQEAAANRKFIWQGTVFPVTMSIGIVPLT
                     RESGDIHAVLAAGDDACHIAKESGGNKISIFQVEDTVFQRRRGEMEWIGKINRAIDEN
                     RFLLYYQPIVPLDPVKGYSKKIEILIRLVNEDGGIARPTDFIPSAERYNLMPQIDRWV
                     IENAMRSFRTLQDRRNPLADSIFSINLSGPSLLDETLVDKILATLAQYSLSATSFCFE
                     ITETAAIQNLAYATRFMKRLKEEGFTFALDDFGSGFSSFGYLKNLPVDYLKIDGSFVQ
                     NIDESLVSCTMVESINSVGHVMGLKTIGEFVKSDKIWKKLVELGVDYGQGYIFAEPRP
                     LLSDPV"
     gene            complement(8050..8571)
                     /locus_tag="P5313_03035"
     CDS             complement(8050..8571)
                     /locus_tag="P5313_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRY79372.1"
                     /translation="MNLLSLAWVFFKIGIAAYGGGWTIVGIIKTEILGRGWITEAGFS
                     DLVAISQVTPGPVALNAATLVGYRLAGIPGAAVATLAVVAFPILSILAATSLLARLGK
                     ARGMLQEALKTGTIALVAMTLWTFLPAAASSWTSGVLAAGAFALAAFTKTHPLFIILG
                     AGGIGLVLSLLVP"
     gene            complement(8568..9122)
                     /locus_tag="P5313_03040"
     CDS             complement(8568..9122)
                     /locus_tag="P5313_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRY79373.1"
                     /translation="MEGTSPTPPAPSPGDIFWTFFRISAVTIGGGYAMVPVIGKEVTA
                     KRWMEEKEFYDLFALAQSFPGPIALNTALIVGRKMAGMAGFCLALAGILVPPFVSVLV
                     VALLLDGFGNLAPVRGFLGGAAAVIPGLVGALLWRMTRTRTWTVWRIACTLCAAAAVI
                     AFRSWAIPLFFASVLVALAGEVRS"
     gene            9410..9982
                     /locus_tag="P5313_03045"
     CDS             9410..9982
                     /locus_tag="P5313_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRY79374.1"
                     /translation="MHQRITLRPGDHRATTDATVLSTLLGSCVAACLYDPAARVSGMN
                     HFLLANRRYARSMPLNVTEAGRYGIHAMELLINDMMKLGAQRSRLQAKVFGGGAILDE
                     GADSHFLCVGDVNQRFIREFLAAEGIRIVSEDLGGELGRVIHFHTDTFQVFRKFIPKT
                     QTIRIERRELGLWKREIERPEEREGAIILF"
     gene            complement(9987..10059)
                     /locus_tag="P5313_03050"
     tRNA            complement(9987..10059)
                     /locus_tag="P5313_03050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10024..10026),aa:Thr,seq:cgt)
     gene            10144..10674
                     /locus_tag="P5313_03055"
     CDS             10144..10674
                     /locus_tag="P5313_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79375.1"
                     /translation="MERRRFVRALPAFFLAVLSAAAAAAEREVDVLVVRLSGGSGEER
                     FRYMDLPAAARFLSPVAFSARDGRLSFQSESSSGILERGRQTLASRPSGTLERISVLL
                     ADPGREPRPSRTVTVTFTLDELLARGGSWSGVPAAWACARAASDSRWTAGRVWARSAI
                     YDAGTGRITVRVALSK"
     gene            complement(10715..14134)
                     /locus_tag="P5313_03060"
     CDS             complement(10715..14134)
                     /locus_tag="P5313_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY79376.1"
                     /translation="MSANPMSSRLDPDQRTAVELRRNGAVSAGAGSGKTTVLAARYLD
                     LVLRDGADVRSILVLTFTRKAAAEMYGRIHRELLASGEPRALEQVDRFSEAQISTLDS
                     FCSLVLRPEAQEYGYPPDFRVDDEECGRIARAQALSFLLERREDPALREVFARLGFER
                     AWKELFADAAARLWTPSAAPDIPAMLRLQADTAERTLREQAALILEEARAVREAGAGS
                     LSAKAASVLDAFATLPAGFAALPTDMIAAALDPVVAFNLQGFGRSDAETRIKEAASAA
                     RDAARRILKAREMEKLAPLTGSVLGLLAELGERVRRAKRGARVMGFRDVAEAAVDLLS
                     RRPGVRAGWKRRFRYIMIDEFQDDDELQKDLLYLLAERADRTDPGVPEVRDLDPDKLF
                     FVGDEKQSIYRFRGADVSVFRGLAEDLGAEGPALRTNYRSEPELVSFFNETFERVLGD
                     AVEPWEARFAPILSRPAAPGGSPSIRYLLKPRRPAAEREGYRSDDEALACGVASFVRK
                     SVEGGELRVPDPGAPGGPGWRAARYDDFAVLLRSTGKQYLLEKYFRLLKVPYSAGDVC
                     GLFTESPANDVYQALRLAVHPEDGPAYAAFLRSPFVRISDDAFVRILAPGLPPFDPEA
                     RCAADLSEDDGARFDRGREILDRLRAGADRLPHSTLVRSLWYEEGLRLGLVRKPDAHP
                     FLEHFDYLFRLAADADARGLALASFVAELEPLTGTPERVPDLDAPREAGSGLRILTVH
                     RSKGLEFPVVILPFLDNAVKPGGNGKAWYLSRNHGITLNLKPWDDPQAKTVNLFYDLA
                     REEESRQEAAELKRLFYVACTRASAHLVFASLEPVRADNLGSSFHTLLAGEDGLRDPG
                     TGLFPHMPAGVREVRVPDLGEEDYGKLFAGGRGASLETLASRYEEARIVRRDWEPRTV
                     SATAVKAAWEVTGKEAWEDLPSLYFDTVAEEVPETVFGELCHAAIQARLDPSGVGTED
                     FEALLSGMPEAHRDALAGEARRLAEGFLGSELGRAAAEAAAAGTLRSEAPFLYAPEVD
                     AGTDRVAAVPVVSGRMDLCFRTGNRVEVVDFKSDRRRRDGVYDGQLAVYRAACRGLYP
                     GAEVRTRLFWLRAARAEERVPAPGEEARLAEAARRAAAPGTGPAPGAPGDR"
     gene            complement(14131..16806)
                     /locus_tag="P5313_03065"
     CDS             complement(14131..16806)
                     /locus_tag="P5313_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY79377.1"
                     /translation="MPSFIESVLAARLPDPSVSFVFPSEVAAASWALRALDLGPSPAV
                     FTDRFLGWDTFKARLLSRKAEGRAADDRTRLLWAASALEANSRSPFLETLLRPAYRSE
                     YEGYVPFVAGILPALRRIAGRAGSRPETGKLRDFGALYARYRSFLSERGLFEPPWEPL
                     PSPEPGLRYDLFCPELARDFEEYRTALSAGTAFRILPLPEAPDCPPLAEFPNLYEELR
                     WIFLSVARDLDSGLRPEEIAVTVPDLDETAPWVEKAAALAGVPAEIRSGRPLARHPVG
                     RFLAALGACSERDFDLESVTALLLDRFLPWKEPDRARSLVRFGILRHAYAPYAADGRK
                     RDPWLESFRICGVPEPGLDSFYRRLKSGARQVTGAADFRSLARAFVAFRREFLDEDRL
                     SEEDARSFQRAMDELSAYSRVESDLDLAAPEGSAFSLFRTVLADQRYVHQSSVPAVSV
                     YSYRVSALLAVRRHYVAGASQEGLRVRFVSAPGLGEEEKEDLGLPDRDASGEYARAYA
                     STGMTVFSFAVEGFRGWNLPFPWFSRAGRVGVPPDGEALRGSDPWSREERAWRGEDFP
                     PVLLADQSRAFRSAARSLGPPASDYRRDRASPQAAGAILTRVTRADGRLRLSATQVSE
                     LLSCPFAWLLARGLALEAEVSGVGFFDALLAGEMGHETLRVLYGRIAASGPFDGRRIP
                     EYLSWIRPSMDEILPAFESRHGPFLRPMFEAYMPKLGDRIERLLREEARVFQGWEVES
                     LEGDLEKDYPDLEAVLVGRLDRLARRDGEHGIIDYKKRSIPGKDDVRPDANGYLAAPQ
                     MAAYILLCEAAGRTVSRARIWSLEDAKALDVVGPGAGTREDYGPALAAFEDGLRKTAE
                     RLRAGDFRPPRPEERDCGNCGWASICRTGYATEGR"
     gene            complement(16924..17436)
                     /locus_tag="P5313_03070"
     CDS             complement(16924..17436)
                     /locus_tag="P5313_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRY79378.1"
                     /translation="MPFESVDLENLSDNPWKLIGKDWMLVTAGNLASWNTMTASWGGL
                     GVLWHQNVAFVFVRPSRHTFEFMNRGDRFTLSFFPEERREILSKCGSVSGRDADKAAL
                     TGLDPFEPEPGVVSFRQARLVLSCRKLYAQDLGPGHIVDPDVLKNYANGDWHRMYVAK
                     VEGAWVDGGR"
     gene            complement(17450..21124)
                     /locus_tag="P5313_03075"
     CDS             complement(17450..21124)
                     /locus_tag="P5313_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY79379.1"
                     /translation="MIGDGGNAMTQRYGIVLRLSAFEGADTEAARPHGPEMEFHVRRT
                     VRAEHKLEESLFSLSGNVVFPNFYASRVLARSLNARPSVQAHPERSVKAGRLNAMGLV
                     DEILHFVCAKFRESVAPDAFVRALAAVEKAVGKAQTDALLLAFTKEFPPRDVYRGKAD
                     EGAWLAGTTEGVPNRVLALEELLLLKLANDNPAFEPFKELFDDTAFRGATKYLPVLES
                     LETFFDGLPVFGPEKRPLVKLLRCPVEASPYSLPGQLDYMRRNWGLLLGPLLARILGG
                     LDMIKEEEKPFFPGPGPSRVYVYEGMEHEYERFTEDMDWMPRVVMLAKSVLVWLHQLS
                     VTYGRTIDRLDLIPDEELDLLASRGFNALWLIGLWERSDASRRIKQLCGNPEAAASAY
                     SLFDYEIAGELGGWSSLHQLRERCAWRGIRLAADMVPNHTGIDSEWVRHRPDLFVQAG
                     HPPFPGYTFNGENLSADPSVGIWLEDHYYNRSDAAVVFKRVDFRTGDTRYIYHGNDGT
                     GMPWNDTAQIDFLKPEAREAVEERILHVARAFPIIRFDAAMVLAKRHFRRLWYPEPGS
                     GGDIASRAEHALSRKDFDVRMPEEFWRQVVDRSARDAPDTLLLAEAFWMMEGYFVRTL
                     GMHRVYNSAFMNMLKKEENAKFKETIKNTQEFDKRILKRFVNFMNNPDEETAVAQFGK
                     GDKYFGVCTLMATLPGLPMFGHGQVEGFEEKYGMEYRRAYRDEAPDSGLVARHEREIF
                     PILKRRHLFSGVDDFLLYDLWDEHGTVNQNVFVYSNGAGGERALVAYNNAYGRASGWV
                     KDSAYYAEILPDGGKRHVQKSLAEALGLSGKEGRFLVFREQRSELWYLRPSKDVAAKG
                     LHLALNGYESQVFLDVHEVEDDATGRYGTLCRRLGGAGAPDLSAALQDIDLAELYAAF
                     AALLSPEFLAALRLPPGTSEKAGPVPGAPASKSKPGKPGKAEAGTAAETRAAPDLDPE
                     TSSAFAALPERCRAFARTALVFLEDFDGGAEALTERPEEAGIKAKKPPAGSAPTDPAE
                     RTAGALAAGFQILTSFLAEARSGGPDSFPASLAAAFPAAAQAAAALVVLGSLRALLGP
                     EARGPDARALADHWCLDRKLREALQSAGMPGDEAWRLAHAAKTAAALHPDKPTVRELW
                     KAWTSDEDGGRILGVNTWDGVEWFRAEGFDLFAPVSALAALFAPGGGTYKTREAAAAK
                     LYSDLRKMEAKSGYKVDGGL"
     gene            21412..22644
                     /locus_tag="P5313_03080"
     CDS             21412..22644
                     /locus_tag="P5313_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79380.1"
                     /translation="MRKIAAVILALALIASAGAQDKVIKIGTLFPMTGPVATAGQRCQ
                     AAVETIVDIINGVYPDLDLPIAKKAGLLDGYRIELVKADHQGKPDVAKSEAERLYGQE
                     GVFAVIGSYNSSASKPASAVAERLQKIFMCGASSSAALTERDLKYFFRLAPTDRIEAI
                     EFVHWFDELNKSKKAGIKTVGVIYENTEFGKHAADEAKAHAQKAGYKVVADVPFTVGA
                     TNLNSEIQKLKAANPDALFGAVLGADYSLMVRTMKQFKWKPKIAINFCSGYQDQKINE
                     QLGKDGWGFMGSAGYTPEFGSRFMKGVAPVEAYFMKKVGVPFDSNSIQEAVAMYVLAL
                     AIEKAGTLDTEKVAEVLRTATFDSPLSMGGKVKFESPGGQNIMARSVISQLQDGAYKT
                     VFPYEYKDAAPIYPIPKW"
     gene            22722..23579
                     /locus_tag="P5313_03085"
     CDS             22722..23579
                     /locus_tag="P5313_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY79381.1"
                     /translation="MSLFFQVILDGLWAGLLYGLIAAGLSLIWGVMDVINFAHGDFLM
                     VGMYVSFFLGFLFKADPLVSWVASGIFLFLFGMLTYKLVISRILGKAMAPLLATFGLS
                     MMVKNLVLNRFSPNFRILESTILEGRSFMVGDVIVPLPQFVTSLFALAVLGLLYWMLT
                     RTRFGWAVQATALDGEAARLMGVNTEKIFLLVFGIGGACVGIAGGLLPSYLATHPDVG
                     TLFSLIAFMIVAMGGFGSVPGALVAAVMVGLIESLAGFYIGPVYKYIAVFGIYLVVIL
                     IRPKGLFGW"
     gene            23583..24593
                     /locus_tag="P5313_03090"
     CDS             23583..24593
                     /locus_tag="P5313_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY79382.1"
                     /translation="MDYKKLLSKFGWPLLLVALAALPLAGLVGPGFSSHVLITVLLTA
                     AMAQAWNLIGGYGGQVSFGHSVFFGIGAYGAAFAVCVGKTSPWPGMIVGAVLAAVTAV
                     IVSYPCFKLKGHYFAIATFAVVEIFNRVFMIWDWVGGAVGLDYTILEDGWRNFTWSVD
                     KRGYYLGALVLFALVFTITRVIERSRFGYYLRAVREGQETAESLGVNSTGVKLTAMAL
                     SAFLAALCGAFFAQYNFRVDPPMVMSLDMSMKFVLVAILGGIGTFWGPLLGALVLIPL
                     QEYSRAYLAKLGSGVDLIIFGLIIILMMVYQPGGILGILTGSRKRQARLRARLAAEGG
                     AK"
     gene            24590..25339
                     /locus_tag="P5313_03095"
     CDS             24590..25339
                     /locus_tag="P5313_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79383.1"
                     /translation="MSEMLQVRGVSMKFGALLANKDVSFDVEEGAIVGLIGPNGAGKT
                     TLFNCVAGFYKPSAGSIRFRGEEIAGLPSYKVARKGLARTFQVVRPLKEMSVFENILV
                     GAYLGHPDNKHARKVAEECLEICFLQEWRDWPAGDMTIGNKKRLEVARALATSPRMIL
                     LDEAVAGLTSTEVREMVEVIKRLKANGLTVLMVEHIMEAIMPIADKIVVLASGEKIAE
                     GAPKDIVRDPKVITAYLGKKYAETMDKGAGA"
     gene            25336..26055
                     /locus_tag="P5313_03100"
     CDS             25336..26055
                     /locus_tag="P5313_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79384.1"
                     /translation="MSGEILLEVKDIRCGYDGVPVIHGVSLEVRSGEIVAIIGANGAG
                     KSTTMRTIAGLMHPLSGVIRFDGADITRTPAYSIVARGLAYVPEARRLFSKLSVRQNL
                     EMGAFTVKDRGEIAARLDRAFQLFPVLKERAGQIAETMSGGEQQMCAIARGLMSNPKL
                     IMLDELSLGLQPSIVEKVLETVVEIRNQGITVLLVEQLVQEALEISDRGYVIQTGAIV
                     HQGTASELLASPEVRKAYMGM"
     gene            complement(26229..26960)
                     /locus_tag="P5313_03105"
     CDS             complement(26229..26960)
                     /locus_tag="P5313_03105"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HRY79385.1"
                     /translation="MERYPEIDALRGFALVCMAVYHLAYDLVIFGVAGLAFWEGFWWW
                     FPRCIAAAFVTLAGLSMALRKARTGAGLRPFLARGLRLAAVAAGISAVTYLALGPGTF
                     VFFGVIHLIAFATLVSYPLADRPAAAAALGAAVLAGGLALGSRRFPGPALAWLGLRPE
                     GYHPADYLPVLPWYAWFLFGIAVAALLYPRGRRSFEPPRTFLDVPPIRALAWLGRRSL
                     AIYLIHLPVLYGIVWGITRAGVAGR"
     gene            27070..27663
                     /locus_tag="P5313_03110"
     CDS             27070..27663
                     /locus_tag="P5313_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79386.1"
                     /translation="MSIVVEHEKRKKEILERALDVFTDEGYEDTTFRKIAERCGITRT
                     ILYLYFKNKREIFSYSIKQFTVGLEADVREFATREDLSNAEKISAILDMVLARCAEEK
                     RLLTVIFGYLDHLRRSGGDPDERVRRRTIRMRHILSTLLIAGIQAGEFRKVPVRAATD
                     LLYSLIEAAIFRIVILGKSEAGTLVDSGHLVVEGMKR"
     gene            27660..28511
                     /gene="lipA"
                     /locus_tag="P5313_03115"
     CDS             27660..28511
                     /gene="lipA"
                     /locus_tag="P5313_03115"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRY79387.1"
                     /translation="MTGPKPAWLRVPIPSGPEWRNMSRLLDRRGLHTVCDSARCPNKA
                     ECWGCGTATFMILGNTCTRNCRFCAVATGRTGDSVNPDEPGQLAQAVAELRLTYAVLT
                     SVDRDDLPDRGAGHFAACVRAIKAGNPGVRVEVLVPDFREGEIDLVLAAGPDAVAHNV
                     ETVPRLQRVRDARASYEGSLHTLERAAAAGIEVVKSSLLLGLGETREEVLGVLDDLRR
                     IGCGSVVLGQYLRPTPSQVPVAEYVTPETFAEYAREARARGFSAVVSGPLARTSYHAR
                     QAFEEVS"
     gene            28508..28795
                     /locus_tag="P5313_03120"
     CDS             28508..28795
                     /locus_tag="P5313_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79388.1"
                     /translation="MTLRRIVKIPAGKLLKLTADVENGVLKSVAVRGDFFAHPEEGFD
                     RAEESLVDIPVGEFRSAFSAALAREGVELFGLSADAAADVFEEMLHDLPPA"
     gene            28776..29537
                     /locus_tag="P5313_03125"
     CDS             28776..29537
                     /locus_tag="P5313_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HRY79389.1"
                     /translation="MTFRLLETGVSPGAFNMGLDEAILGAVAEGRQPPTLRLYGWNPP
                     TVTLGYFQGLDEEVDREACARMGVDVVRRITGGGAVFHDAELTYSIVLPESHPRARPG
                     ILDSYRVLCAGVVEGLARLGLSGEFAPINDVLVGGRKVSGNAQTRKRGCILQHGTILL
                     DVDVDRMFALLKVPSEKMKGKLIEDVKQRVTSLRHQLGRSVGLPEAAAAFAAGFAAAL
                     DVVLDPAQPSPEELEEGRRIAREKYGTEAWTGRRK"
     gene            complement(29655..30395)
                     /locus_tag="P5313_03130"
     CDS             complement(29655..30395)
                     /locus_tag="P5313_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRY79390.1"
                     /translation="MPVPTSRTLFRSLAVLAAALAAASLTVVVSSCMSSVTAVEPEAY
                     VPEEFRKFSSYDETEYVQAFRSHAPEDFIAAGYGNPLYRESVLAFFSSITKSTEIAQA
                     ILDNAVEYSVPVSLAFALAFEESQFHPRAINRNAESVDRGLFQLNSKSFPNMTIEQFY
                     DPVLNAKKGIAHLAYCLDEGGNEVAALAIYNAGYGRVSKGGTPRKTLDYISRITKYAS
                     NLEALFEAQVLARRIAAAKDGTRGTKVE"
     gene            30612..31178
                     /locus_tag="P5313_03135"
     CDS             30612..31178
                     /locus_tag="P5313_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY79391.1"
                     /translation="MLNRDEAIRLWRTHNQDESLFRHALSVEAALRRFAVRYGQDPDW
                     WGLVGLLHDIDYERWPEEHLKHARTLLAGAGLPEDFIRAVESHGWGLCTDVEPKSDME
                     KVLYAVDELTGFIAACAFVRPSRSVLDMEVKSVRKKWGAAAFAAKVDRSVIEKGAAML
                     GLSLDELIGETILALRDASEAVGLKGTL"
BASE COUNT         4979 a        10487 c        10914 g         5014 t
ORIGIN      
        1 gcaattcgtc caactgtttc tggtccacct cattggggca atcatccatg ggactcacgg
       61 ccaaggagtt cttggggaag gcgatgactt ccttgatgga agtgtccccg gccatgagca
      121 tgaccaggcg gtccagaccg ggggcgatgc cgccgtgggg cggggggccg tacttgaagg
      181 cctcggtcag gaagccgaac tttttctggg cgtcctcttc ggagaagccc acgatcttga
      241 agacccgctt ctggaggtcc gggtcgtgta tccggatgga accggaggcg agctcgtacc
      301 cgttgaggac gaggtcgtag aggtcgccct tcacggagcc ggggtcggac tccatcgtgg
      361 cgtggaacct ctcctggggc atggtgaaca tgtggtgggc cgcttcccag cggttctcgt
      421 cctcgttcca ctcgaacatg gggaagtcga cgatccaggc gaactcgaaa cgcttggggt
      481 cgcagagacc gaggtccttg ccgagcttgg aacggacggc ccccagggac tggcaggcga
      541 ccttgcgctt ggcgtccgcc accatcagta cgaggtcccc ggacttcgcc cccagggccg
      601 cgaccaggtc cgaggcgagt cccgccgggg cgccctcgcc ggcgaagaat tttgaagccc
      661 cgccttcgag ttgcgcggcc gcgccgcctt ctccggccgc gaccttgaac cacgccatgc
      721 ccttggcgcg gtataccttg gcggccgcct cgagctcctc gatctgcttg cgcgagtagt
      781 cggccttgtc cggggcgacc agggccttga cggcgccccc cgcggccagg gcgtccttga
      841 aagcctggaa ggtcgagcgg gcggcgaggt ccccgatgtc cttcatcgcg aggccgaagc
      901 ggaggtcggg cttgtcggtc ccgtacagct ccatggactc gtcgtaggag atgcggcgga
      961 agttcgcggg cagctcctcc cccagggtct tgcggaagac atggccgaag agccgctcca
     1021 ccatggaaag gacgtcgtcg cgcttgacga aggacatctc gatgtcgatc tgggtgaatt
     1081 cgggctggcg gtccccccgg gcgtcctcgt cccggtagca ccgggcgatc tggaagtact
     1141 tgtccatccc ggagaccatg aggatctgct tgtagagctg ggggctctgg ggcagggcgt
     1201 agaacttccc ggggtagagg cgggagggca cgaggtagtc ccgggcgccc tcgggggtgg
     1261 agcggatgaa ggtgggggtc tcgatctcgt agaagccctc ggaggccagg tgctcccgca
     1321 ccgcgaaggc gacctcgtgg cgcaggcgca tccggtactg catggccccg gaccgcaggt
     1381 ccaggtagcg gtacttgagg cgggtctcct ccttggcgtc ggaccgctcg tcgatctgga
     1441 aggggagggt ctcgcagcgg gtcaggatct ggagctcgct caccacgacc tcgatctccc
     1501 cggtgggcat gtcgggattc accatggaat cgggtcgggc gcggaccctg ccgcggacgg
     1561 acaggcagta ctcgttcttg agctccgccg ccgaggcctt cagggcagcc tcggcgtccg
     1621 cgtccaccac gacctgggtg atcccgtagc ggtcccggag gttgatgaag gagattccgc
     1681 cgtggtcccg cttgcggtgg acccagccgt tcagtgcgac gatctgcccc gcgtcgccct
     1741 tgcgaagggc cccgcaggtg gtggtgcgct tgaggtattc catgaggcgg cattgtatac
     1801 ttgcccgccg ggggcgcgca agggaggccg ccgttccggc tccgcttgcc ccgggttccg
     1861 ggggccggta taatccgcgg ccatgagata cctcctggtc cggcacggtc ccccccgctc
     1921 gccctacaac ttcgaggccg acctggccgc ctccggcgcc cgcctcgata cggtcctctt
     1981 atcggagggc gaccgccccg ccgccccggc cgcctacgac gctgtggtcg tgttcggggg
     2041 ctacatgtac gccaccgagg acgacaagta cccctggatc cgggacgagc tggggtacat
     2101 ggagtccgcg atcaaggcgg gggtcccgct cctggggatc tgcctgggag gccagctgtt
     2161 ggcccgcctg ctgggggccc gggtctacaa gaccccggtg cccgagttcg gctactggcc
     2221 catcgagcgg accgaggccg ggcggagctc ggtcctgttc gagggcctgc ccgagcccct
     2281 gctcggattc ctgtggcaca acgacgcctt cgacctgccc tccggggccg tgcgcctggc
     2341 ccggacggag cactgcccga accaggcctt ttcctacggg gaccggacct tcggcctgca
     2401 gttccacctg gagttctccg gggaccagct ccggtccatg gtcacccggg acgccgcgtc
     2461 cctgccggcc cccagcccgg tccggcagga ccccgccgcc gtggccgcga acgaggccgc
     2521 ccacgccgag gtgaagtcat ccatgctccg gatgctccgg acggtaggcc aactccggga
     2581 aactcgacga agcccctagg ggcttcgttg ccgttgggga ccacaacgtg gtccccaacg
     2641 ctatgcggcc cagagccgga tcaggcggag caccgtccgg acggtcagga ccatctcgga
     2701 caggctggcc cactcgtagc gggagtggta gttgtagccc ccggtgaaga tgttcggggt
     2761 ggggatgccc atctccgtga gccgggcccc gtcggtgccc ccccggatcg gcttgatcac
     2821 cggcgggatc ccgacgtcct tgcaggcccg gaccaggcgc tcgaggacct cgggccgctc
     2881 gtccagcttc ctgcgcatat tcacgtactg cttcttgatg tccgcctgca cggagccgcc
     2941 cgggaactgg gcttccacgg ccttggccag ggccttgacc gtctcgatcc ggcggttcat
     3001 cccctcgtcc gtgaagtccc ggaggaagac ctccacggag gcgctctcca gggtcccctg
     3061 gacctcgatg gggcagtagt agccgtacca gccgtcggtc gactccgggc tctccgcccg
     3121 gggcagcatc gagatgaagg aggcggccat ggcggtggcg ttggccagct tgccccgggc
     3181 cgacccgata tggatcacgt accccttgaa ggtcagcttg gccgaatagg cggtgaaaca
     3241 ctccgcctcg atctccccca gcttgccgcc gtccagggtg tagcaggcca ccgagcggac
     3301 cttcgcgagg gggaagaggt tcatgccctt gccggtctcc tcgtcggggg tgaagaagac
     3361 ctcgacgggg ccgtgggcga tctccgggtg ggcgatgagg tgccccaggg cggtcaggat
     3421 ctccgccacc cccgccttgt cgtccgcccc cagaagggtc gtcccgtccg tgacgatcag
     3481 ggtgtccccg gcgtggtccg ccaggtccgg gtaggccgcg gggtccaggg tatgctcgtc
     3541 ggacagtttc agggccttcc cgtcgtagtc cgggacgacc cggggcttgg cgtccttcgc
     3601 cgtgacgtcg ctggcggtgt ccatgtgggc catgaacccg acggagggct tgccctcgta
     3661 cccgggggtc gcggggagcc gggcggaaag gtaacagtgg tcgtccaggg tgacgtcgga
     3721 gaaccccagg tcctggagtt ccttctggag gacccgggcc aggtcccact gggtcggggt
     3781 ggaggggatg tcctccacat gccggtcgct ggtcgtctcg atccgggcgt agcgcgtaag
     3841 gcgctcggcc agctccccgg cgaaggggtc cgaaagcgct tccttgtagt tgtccatgcc
     3901 cggcagtatg tcccggtttc cgggattggg caagtacgga ggcgaccgga cggtcgcggc
     3961 cttgcccccg ggaggggcaa tcgatatatt cttcccgatg gcaatacccg aataccccga
     4021 cttcgccccg atctccctgg acatgcggga cgagatgtac cccagcctga accttctttc
     4081 cgacggaatc tcggagttca ccttctccaa tctcttcctc ttccggcacg cctacggcta
     4141 caaggtgtcc cgcatccccg gccagaccct ggtcctgagg ggggtcaaga acggggagcc
     4201 cttcttcctg accccctgcg cggtgccgga ccgggagcac cttgaggacc ttttcaagga
     4261 ccatctgtac ctcaagaacc tctccgagag ccaatcccag gtccaccggg tggagctgga
     4321 gcagtggggc taccgcatcc aggaggaccg ggacaacttc gactacctct accacaggac
     4381 ggacctggcg gagctctccg gaaaggccta ccacaagaaa cggaacctgg tgaacgcctt
     4441 cctgggcacc tgcgcctgcg aacagaggcc cctgaccaag gccaacgtgg cggacgccgt
     4501 tgaggtcctg gaccggtggc gcgaggccaa gggggtcgag ggggactacg ccgcctcccg
     4561 ggaggccctg gagtatttcg aggagctggg gatgcgggga gcggtgtact acgccgacgg
     4621 ccgccccgcg ggctacgtcc tcggagaacc catctccaag gcccggatgt tcgcggtcca
     4681 cttcgagaag gccgtggacg ggtacaaggg gatctaccag ttcatcaacc aggccttcgc
     4741 ccagaccctg cccgcgtatt tccggatcgt gaaccgggaa caggacctgg gggacgaggg
     4801 gcttaggcag gccaagatga cctaccgtcc cttcgggttc gtgaagaaat accgggtcta
     4861 tagagcctag gtccgttccg cactatactg cttccatgac atcctccctg gtacggctca
     4921 cgggaaccga tcacgacaag gtcttcggac ttcgggatac cctcctggcc cgggccctct
     4981 ccgagaaccg gagggggatc cgttccctgc ccgagtacct cgagtacctg gcgggacgga
     5041 cggtctgcct gaccctcctg gcgggagccg ggtcccgatg ggtggactcc ctgagacagg
     5101 ccgccgccga gggccgggac ctccgcttcg atcccgcccg gccccggggc ctcttcccgg
     5161 tgcgcaacgc cctgggccgg gggccggatc ccgtccccat cgcggcctac gcggtggacg
     5221 ccttccgggg cctggggagc cgggtgatcg tggtccgggg ctgggaagcc gagatcgacc
     5281 gggagatcct ggaaccctta ggtgtctccc cggaggagcg gcgcttcttc acccaggagg
     5341 cccccctggg caagcccctg ggccacgggg acgcggtctg gcagtgccgg gacctctgga
     5401 aggacgcgga gtacgtcctg gtcaatttcg ggggggacgc gtcgagcccc ttcacggccc
     5461 gggccgccct ggtcgtcctg gacgccctcg tctccctggg ggagcccgtc ggcctcctgc
     5521 ttcccgccgc ccgcttcgaa cgaccggcgt acccgataac cctggacggc ctcggacggc
     5581 cggcttcctt cggccacgcc aagctctccg gaaccgcggt ccggtcctcc tcgggctggg
     5641 ccaacgtggg cctgcgggcc tacaaggccc cgtccctcct cgccctgtgc gagacgatcc
     5701 gggccgagca ctggaaagac ggggagggct actcgattcc cgggaacgat ccggccggga
     5761 gggagttcgc cctggacaac gtggacgccc ggctggccga aggacgcttg gtacgcctcc
     5821 tggccgcggc cctgccggag gagctttccc cggtgaagag cctggcggac gtcccggcct
     5881 tcgaggaggc ggccgcccgg gtgcggcggg acgcggccgc cctgccgggc ggggatccct
     5941 gaccggctag acgggatcgg agagtagggg ccggggctcc gcgaagatgt agccctggcc
     6001 gtagtccacc cccagctcga ccagcttctt ccagatcttg tcggacttca cgaactcccc
     6061 gatggtcttg agccccatga cgtggcccac ggagttgatg gattccacca tggtgcagct
     6121 caccaggctc tcgtcgatgt tctgcacgaa ggacccgtcg atcttgaggt agtccacggg
     6181 caggttcttg aggtagccga aggatgagaa ccccgagccg aagtcgtcca gggcgaaggt
     6241 gaacccctcc tccttgagcc gcttcatgaa gcgggtggcg taggcgaggt tctggatggc
     6301 cgccgtctcg gtgatctcga aacagaaaga ggtggcggac aggctgtact gggccagggt
     6361 ggccaggatc ttgtcgacca gggtctcgtc caggagggac ggcccggaca ggttgatcga
     6421 gaagatggag tccgccaggg ggttcctccg gtcctggagg gtgcggaagg accgcatggc
     6481 gttttcgatc acccagcggt cgatctgggg catcaggttg taccgctccg cggaggggat
     6541 gaagtccgtg ggacgggcga tccccccgtc ctcgttcacc aggcggatga ggatctcgat
     6601 cttcttggag tatcccttca cgggatccag ggggacgatg ggctggtagt agagcaggaa
     6661 gcggttctcg tcgatggcgc ggttgatctt gccgatccac tccatctcgc cccggcgccg
     6721 ctggaagacc gtgtcctcca cctggaagat gctgatcttg ttgcccccgg attccttggc
     6781 gatgtggcag gcgtcgtccc ccgcggccag gacggcgtgg atgtccccgg attcccgggt
     6841 cagcgggacg attccgatgg acatggtgac cgggaacacc gtcccctgcc agatgaattt
     6901 acggttggcc gcggcctcct ggaggcgctt ggccacgttg aaggcgtcct ggacggcgca
     6961 gttcatcagg atgcaggcga actcgtcccc cccgatccgg gcggagatgt cgttccgctg
     7021 gatgttggcc tggatgtggg acgccacctg ccgcaggagc tcgtcccccg ccagggcccc
     7081 gcaggtgtcg ttgaccgcct tgaagcggtc cacgtccagg atcagaaggc cgtggctgtc
     7141 tccggtctgc tggatgttct tgagcacctc cccgagcttg aaggagagct cctcccggtt
     7201 ggacagtccg gtcaggatgt cgtggctggc ctggtagttg atggtctcgg acatccgctt
     7261 catctcggtg atgtcccgca gggccaggag gtagccctcc gtcgcgttct ccttctcgtg
     7321 gatccgggtg atggagccgt ccaccaggat ggtctggttg aggttgttct tgatggacac
     7381 ctcgtggaag aagtaggggc gttcctccac cgggggcgct ccgctgccca ggaggtcctt
     7441 ctgggttcgg gggtcgaaga gggtcaggat gcgggtcagg caggcgccct ggacggcctc
     7501 ctcccggtac cccgtgatct cctccgccac ggtgttcatg aactggatgt tgaggttcat
     7561 gtccgtggcc acgatcccgt cgttgatgga atggaggatg gccgagaaga gccgctcctg
     7621 cttccgcagg cgcttctcga tcccgtactt gtacagggcg atgtcgatgg tggtgtagag
     7681 ttcccgttcc ttgaggggct tcaggatgta gccgtagggt tcgacttcct tggcccgctc
     7741 cagggtcctc tcgtccgtat aggcggtcag gaagatgacc ggcaggccca gctggttgcg
     7801 gatgatcccc gcggcgtcga tgccgtccat ggtgccggcc aggtagatgt ccatgaggac
     7861 gatgtccggg cccgcctggc gggccagctc cacggccgtc tcgccctcgc tggccatccc
     7921 gaccaccgtg tacccgaagc gctccagcct gcgctggagg tccagggcga tgatcttctc
     7981 gtcctcgacg atcagtatct tctcgttgtt catggcgtac tggaagtatc gttccctcgt
     8041 tgccccgggt caagggacaa ggagggagag gaccagtccg atcccccccg cgcccaggat
     8101 gatgaacagg gggtgggtct tggtgaaggc ggccagggcg aaggctccgg cagccaggac
     8161 cccggaggtc caggacgagg ccgccgcggg caggaaggtc cagagggtca tggccaccag
     8221 ggcgatggta cccgtcttca aggcctcctg gagcatgcct cgggccttgc ccagccgggc
     8281 caggagactc gtggccgcca ggatggacag gatcgggaag gccaccacgg ccagggtcgc
     8341 gacggccgcc cccgggatcc ccgccaggcg gtaccccacc agggtagcag cgttcagggc
     8401 caccgggccc ggggtcacct gggagatcgc cacgagatcg gaaaaccccg cctcggtgat
     8461 ccagccccgg ccgaggatct cggtcttgat gatgccgacg atggtccaac cgcccccgta
     8521 ggccgcgatg ccgatcttga agaagaccca ggccaggcta agcaggttca cgagcggacc
     8581 tccccggcca gggccaccag gacggatgcg aagaagaggg ggatggccca ggaccggaag
     8641 gcgatgaccg cggccgccgc gcacagggtg caggcgatcc gccagaccgt ccaggtgcgc
     8701 gtccgggtca tgcgccagag cagggcgccc accaagccgg ggatcaccgc cgccgccccg
     8761 cccaggaagc cccgcaccgg cgccaggttc ccgaagccgt ccagaaggag ggccaccacc
     8821 aggaccgaga cgaagggggg caccaggatg ccggccaggg cgaggcagaa gccggccatg
     8881 ccggccatct tcctccccac gataagggcc gtgttcaggg cgatgggacc cgggaaggac
     8941 tgggccaggg cgaagaggtc gtagaactcc ttctcctcca tccaccgctt ggcggtcacc
     9001 tccttgccga tgaccgggac catggcgtag cccccgccga tggtcacggc ggagatgcgg
     9061 aagaatgtcc agaagatgtc gccggggctc ggggcgggcg gggtgggcga ggtgccttcc
     9121 atggtatctc cggaatggct ggattcgagg gggcatcggc cggaggtcca cggccggctc
     9181 ccggagccgc cccatcatac aggggggcgg ggcggctgta aagaagcgga tcccccccca
     9241 ggtccgccgg gcatcgtgcc gggcggcggg gaacgcggct tcccttgtac ccgcggactc
     9301 gggaccgtta tatttgtaga aaagagtact atccacgcgc gggcctgcgc ccggccgcgg
     9361 tacgggaatc cggaaaccga acgggagacg tatgggatac tcggttccgt tgcaccagcg
     9421 aatcaccctt cggcccgggg accatcgggc caccacggac gcgacggtcc tctcgaccct
     9481 gctgggatcc tgcgtggccg cctgtctcta cgatcctgcg gcccgggtgt ccgggatgaa
     9541 ccacttcctt ctggccaacc gacggtacgc ccgaagcatg cccttgaacg tcaccgaggc
     9601 cggacggtac gggatccacg ccatggagct cctgatcaac gacatgatga agctcggggc
     9661 ccagcggtcc cgtctgcagg ccaaggtctt cgggggaggg gccatcctgg acgagggggc
     9721 ggactcccac tttctctgcg tcggggacgt gaaccagcgc ttcatccggg aattcctggc
     9781 cgccgagggc attcggatcg tctccgagga cctgggaggg gagctgggcc gggtgatcca
     9841 tttccatacg gacaccttcc aggtcttccg caaattcata cccaagaccc agaccatccg
     9901 gatcgagcgg cgggagttgg gcttgtggaa acgggagatc gagaggcccg aggagcggga
     9961 gggagcgatc attcttttct agctaaagcc gagaagcgga atcgaaccgc tgacctgcgc
    10021 attacgagtg cgccgctctg ccatctgagc tatctcggcg ggcggtcgag actataccgg
    10081 aagagggtca aagaatcaag gggaaccggg aatcccgccc ggggccgcgc tatcttctcg
    10141 gtcatggaac gaagacggtt cgtgcgggcc ctgcccgcct ttttcctggc cgtcctgtcc
    10201 gcggcggccg ccgcagcgga gcgggaggtc gacgtcctgg tggttcggct ctccgggggc
    10261 tccggggagg aacgcttccg ctacatggac ctcccggcgg cggcccggtt cctgtccccc
    10321 gtggccttct ccgcacggga cggccgcctc agcttccagt cggagtcctc gtccggcatc
    10381 ctggagcgcg gccggcagac cctggcctcc cggccttccg gcaccctcga gcggatttcc
    10441 gtgctcctgg ccgaccccgg ccgggaaccc cggccttccc ggacggtcac ggtcaccttc
    10501 accctggacg agctcctggc ccggggggga tcctggtccg gggttccggc ggcctgggcc
    10561 tgcgcccggg cggcttcgga ttcccgctgg accgccggca gggtctgggc ccggtccgcg
    10621 atctacgacg ccgggacggg caggataacc gttcgggtgg ccctgtcgaa gtagggtccg
    10681 ggccgccgga gcggcaaccg cgggcccggg cgggtcatcg atctcccggg gccccgggcg
    10741 cggggccggt acccggggcg gccgcccgcc gggccgcttc ggccaggcgg gcttcctccc
    10801 cgggggcggg gacccgttcc tccgcccggg ccgcccggag ccagaagagc cgggtccgca
    10861 cctcggcccc ggggtagaga ccccggcagg ccgcccggta gaccgccagc tggccgtcgt
    10921 agaccccgtc ccggcggcgg cggtcgctct tgaagtccac cacctccacc cggttcccgg
    10981 tccggaaaca caggtccatg cgccccgaga ccacggggac ggccgccaca cggtccgtcc
    11041 cggcatccac ctccggcgcg tacaggaagg gcgcctccga ccgcagcgtc ccggccgcgg
    11101 cggcctccgc ggcggcccgc ccgagctcgg agcccaggaa gccctccgcc agacggcggg
    11161 cttccccggc cagggcgtcc cgatgggcct cgggcatccc ggacaggagg gcctcgaagt
    11221 cctccgtccc gacgccggag gggtccagcc gggcctggat cgcggcgtgg cagagctccc
    11281 cgaagaccgt ctccggaacc tcctctgcga cggtgtcgaa gtacagggag ggaaggtcct
    11341 cccaggcttc cttcccggtc acctcccagg ccgccttgac cgcggtggcc gagaccgtcc
    11401 ggggctccca gtccctccgg acgatccgcg cctcctcgta ccgggacgcc agggtctcga
    11461 gcgaggcgcc cctacccccc gcgaaaagtt ttccgtagtc ctcctcgccc aggtccggaa
    11521 cccggacctc ccggacgccg gccggcatat gcgggaacaa accggtcccc gggtcccgca
    11581 ggccgtcctc tcccgccagg agggtatgga aggaggaacc cagattgtcc gcgcgcacgg
    11641 gctccaggga ggcgaagacc agatgggcgg aggcccgggt gcaggccacg tagaagagcc
    11701 gtttgagctc cgcggcctcc tgccgggact cctcctcccg ggccaggtcg tagaagaggt
    11761 tcacggtctt ggcctgcgga tcgtcccagg gcttgaggtt cagagtgatc ccgtggttcc
    11821 gggacaggta ccaggccttt ccgttccctc cgggtttcac ggcgttgtcc aggaagggca
    11881 ggatgaccac cgggaactcc aggcccttgg acctgtggac cgtcaggatc cggaggccgg
    11941 acccggcctc ccggggggcg tccaggtccg ggacccgttc cggggtgccc gtcaggggct
    12001 ccagctcggc aacgaaggaa gccagggcca gcccccgggc gtccgcgtcc gccgccaggc
    12061 ggaagaggta gtcgaagtgc tccaggaagg gatgggcgtc gggtttccgg acaaggccca
    12121 ggcgcaggcc ctcctcgtac cacagggatc ggaccagggt ggagtgcgga aggcggtcgg
    12181 caccggccct aagccgatcc aggatctcgc ggccccggtc gaaccgggcc ccgtcgtcct
    12241 cggagaggtc cgcggcgcac cgggcctcgg gatcgaaggg aggcaggccc ggggccagga
    12301 tgcggacgaa ggcgtcgtcg gagatccgga cgaaggggga gcggaggaag gccgcgtagg
    12361 cgggcccgtc ctcgggatgg accgccaggc ggagggcctg atagacgtcg ttggccgggg
    12421 actcggtgaa gaggccgcag acgtccccgg ccgagtaggg gactttcagg agtcggaagt
    12481 acttctccag gaggtattgc ttgccggtgc tccggagaag gaccgcgaag tcgtcgtacc
    12541 gcgccgctct ccaaccgggg cctcccgggg cgcccggatc cggaaccctc aactccccgc
    12601 cttccacgga tttccggacg aaggacgcga ccccgcaggc cagggcctcg tcgtcgctcc
    12661 ggtagccttc ccgttccgcg gcgggacgcc ggggcttcag gaggtaccgg atcgagggcg
    12721 agcccccggg ggcggcgggg cgggagagga tcggggcgaa ccgggcctcc cagggctcga
    12781 cggcgtcccc caggacccgc tcgaaggtct cgttgaagaa ggaaaccagc tcgggctcgg
    12841 agcggtagtt cgtcctcagg gcgggaccct ccgcccccag gtcttccgcc aggccccgga
    12901 agaccgagac gtcggccccc cggaagcggt agatgctctg cttctcgtcc cccacgaaga
    12961 agagcttgtc cgggtccagg tcccggacct ccgggacccc gggatccgtc cggtccgcgc
    13021 gctccgcgag gaggtagagc aggtccttct gcagctcgtc gtcgtcctgg aactcgtcga
    13081 tcatgatgta ccggaaccgc ctcttccacc cggcccggac cccgggcctc cgggacagga
    13141 ggtccaccgc ggcttccgcc acgtcccgga accccatcac ccgggctccc cgcttggccc
    13201 ggcggacccg ctcccccagc tcggccagga ggcccaggac ggaacccgta aggggcgcca
    13261 gcttttccat ttcccgggcc ttgaggatcc ggcgggccgc gtcccgggcc gcggacgcgg
    13321 cttccttgat ccgggtctcg gcgtccgaac gcccgaagcc ctggagattg aaggccacga
    13381 cgggatccag ggccgccgcg atcatgtccg tgggcagggc cgcgaagccc gcgggtaggg
    13441 tcgcgaaggc gtccaggacg gaggcggcct tggcggaaag ggacccggcg cccgcctcgc
    13501 ggacggcccg ggcttcctcc aggatgagcg ccgcctgttc ccgtagggtc cgctccgcgg
    13561 tatccgcctg gagccggagc atggccggga tgtccggggc ggcggagggg gtccagagcc
    13621 gggcggccgc gtccgcgaag agctccttcc aggccctctc gaagcccagc cgggcgaaga
    13681 cctcccggag ggcgggatcc tcccggcgct ccaggaggaa ggacagggcc tgggcgcggg
    13741 cgatgcggcc gcattcctcg tcgtccaccc ggaagtccgg gggatatccg tactcctggg
    13801 cctccggtcg aaggaccagg gagcagaagg agtccagggt ggagatctgg gcctccgaga
    13861 agcgatccac ctgctccagg gcccggggct cgccggaggc caggagctcc cggtggatgc
    13921 gtccgtacat ctccgcggcg gccttgcggg tgaaggtcag gaccaggatg gaccggacgt
    13981 ccgccccgtc ccgcaggacc aggtccaggt accgggcggc caggaccgtg gtcttcccgc
    14041 tcccggcccc cgcggacacg gctccgttcc tccggagctc cacggcggtc cgctggtccg
    14101 gatcgagccg gctcgacatc gggttggcgc tcatcgtccc tccgtggcgt agccggtccg
    14161 gcagatcgag gcccagccgc agttcccgca gtcccgctcc tccggacggg gaggacggaa
    14221 gtccccggcc cggagccgtt ccgcagtctt ccgcaggccg tcctcgaagg ccgccagggc
    14281 cggaccgtag tcctctcggg tcccggctcc ggggcctaca acgtccaggg ccttggcgtc
    14341 ctccagggac cagatccggg cccgggatac ggtccggccc gccgcctcgc acagaaggat
    14401 gtaggcggcc atctggggtg ccgccaggta tccgttcgcg tcgggccgga cgtcgtcctt
    14461 gcccgggatc gagcgcttct tgtagtcgat gatcccgtgc tccccgtccc ggcgggccag
    14521 gcggtccagg cgcccgacca ggaccgcctc caggtccggg tagtccttct ccaggtcgcc
    14581 ctccaggctc tcgacctccc agccctggaa gacccgggcc tcctcccgta ggaggcgctc
    14641 gatccggtct cccagcttgg gcatgtaggc ttcgaacatg ggccggagga aggggccgtg
    14701 ccgggactcg aacgcgggga ggatctcgtc catggaaggc cggatccagg ataggtactc
    14761 cgggatccgg cgtccgtcga aggggccgga agcggctatc cggccgtaca aaactcggag
    14821 ggtctcgtgg cccatctccc cggccaggag ggcgtcgaag aagcccaccc cggacacctc
    14881 cgcttccagg gccaggcccc gggccaggag ccaggcgaag gggcaggaca gcagctccga
    14941 aacctgggtg gccgagaggc gcaggcgccc gtccgcccgg gtgacccggg tcaggatcgc
    15001 cccggcggct tggggggagg cccggtcccg gcgatagtcc gaggccgggg gccccaggga
    15061 ccgggccgcg gaccggaagg cccgggactg atccgccagc agtacggggg ggaagtcctc
    15121 gccccgccaa gcccgctcct cccgggacca ggggtccgag ccccggaggg cctccccgtc
    15181 cggcgggact ccgacccggc cggcccggga gaaccagggg aacgggaggt tccagccccg
    15241 gaagccctcg acggcgaagc tgaagaccgt cattcccgtg gacgcgtagg cccgggcgta
    15301 ctccccggag gcgtcccggt ccgggagtcc caggtcctcc ttctcctcct ccccgagtcc
    15361 gggggcggag acgaagcgaa cccgcaggcc ttcctgggat gccccggcca cgtagtgcct
    15421 gcggacggcc aggagggcgg agacccggta ggagtacacg gacacggcgg ggaccgagga
    15481 ttggtggacg tagcgctggt ccgccagcac cgtacggaag agggagaagg cggaaccctc
    15541 cggggcggcc aggtccaggt cggactcgac gcgggaatag gcggagagct cgtccatggc
    15601 ccgctggaag gaccgggcgt cctcctcgga aaggcggtcc tcgtccagga attccctgcg
    15661 gaaggcgacg aaggcccggg ccagactccg gaaatccgcg gccccggtga cctgccgggc
    15721 gccggacttg agcctgcggt agaaggagtc caggccgggt tccgggacac cgcagatccg
    15781 gaagctttcc agccagggat cccgcttccg gccgtccgcg gcgtaggggg cataggcgtg
    15841 ccgcaggatt ccgaagcgca ccagggaacg ggcccggtcc ggctccttcc agggcaggaa
    15901 gcggtccagg agcagggcgg tgaccgattc caggtcgaag tccctctccg agcaggcgcc
    15961 cagggcggcc aggaagcggc cgaccggatg ccgggccagg ggccggccgc tccggatctc
    16021 cgcgggcacc cccgccaggg cggcggcctt ctccacccag ggggcggtct cgtccaggtc
    16081 cgggaccgtg accgcgatct cctcggggcg cagccccgaa tccaggtccc gggctacgga
    16141 gaggaatatc cagcggagct cctcgtacag gttcgggaat tccgccaggg gggggcagtc
    16201 cggggcttcc gggagcggca ggatgcggaa cgccgtcccc gcggacaggg ccgtccggta
    16261 ctcctcgaag tcccgggcga gctccgggca gaagaggtcg tagcgcaggc cgggctccgg
    16321 ggagggcagg ggttcccagg gcggttcgaa gaggccccgc tcggacagga aggaccggta
    16381 ccgggcgtac agggccccga aatcccggag cttgccggtt tccggtcggg atccggcccg
    16441 gccggcgatc ctgcgcaggg ccgggaggat cccggccacg aagggaacgt agccctcgta
    16501 ctcggaacga taggccggcc gcaggagggt ctccaggaag ggggaccggg agttggcctc
    16561 cagggcggag gcggcccaga gcagacgggt ccggtcgtcc gcggcccggc cctcggcctt
    16621 ccgggagagg agccgggcct tgaaggtgtc ccagcccagg aagcggtccg tgaagaccgc
    16681 cggggagggc cccaggtcca gggcccgcag cgcccaggac gccgcggcca cctcgctggg
    16741 gaagacgaag gagaccgagg ggtcgggaag ccgggctgcc aggacggatt cgatgaagga
    16801 cggcataggt cagagggaag tgaacggtgg acagcggaca gtgaaccgag gcgaagcttc
    16861 cgcccggatc ccgcgtatcg ttaaacggtc gtatgattca agtgttctgc cgtacatttc
    16921 cgttcaccgt ccaccgtcca cccacgctcc ctccaccttg gcgacgtaca tccggtgcca
    16981 gtccccgttg gcgtagtttt tcaggacatc ggggtccacg atatggccgg ggcccagatc
    17041 ctgggcgtag agcttgcggc aggaaaggac gagccgggcc tggcggaagg agacgacccc
    17101 cggttcgggc tcgaacgggt cgaggcccgt gagggccgcc ttgtccgcgt ccctccccga
    17161 gacgctcccg cacttggaaa ggatctcccg ccgttcctcg gggaaaaagg agagggtgaa
    17221 acggtcgccc cggttcatga actcgaaggt gtgccgggag ggccggacga agacgaaggc
    17281 cacgttctgg tgccagagga cccccaggcc gccccagctg gcggtcatgg tgttccagga
    17341 ggcgaggttc cccgcggtca ccagcatcca gtccttgccg atgagcttcc agggattgtc
    17401 cgaaaggttc tcgaggtcga cggattcgaa gggcatggtc actccttggt tacagtccac
    17461 cgtccacttt gtaccccgac ttcgcctcca tcttccggag gtcggaatac agtttggcgg
    17521 ccgcggcctc ccgggtcttg taggtcccgc cgccgggagc gaagagggcg gccagggccg
    17581 agacgggggc gaacaggtcg aaaccctcgg cccggaacca ctcgaccccg tcccaggtgt
    17641 tcacccccag gatgcgtccg ccgtcctcgt ccgaggtcca ggccttccag agctcccgga
    17701 ccgtcggttt atccggatgg agggccgcgg cggtcttggc cgcgtgggcc aggcgccagg
    17761 cctcgtcccc gggcatcccc gcggactgga gggcttcccg gagcttgcgg tccaggcacc
    17821 agtggtccgc cagggcccgg gcgtccgggc cccgggcctc gggtccgagg agggcgcgca
    17881 gggaacccag gacgaccagg gccgcggcgg cttgggcggc cgcggggaag gcggcggcca
    17941 gggaggcggg gaaggaatcc ggtccgccgg accgggcttc cgccaggaag gacgtcagaa
    18001 tttggaatcc tgcggccagg gcacccgcgg tccgctccgc cgggtccgtc ggggcggatc
    18061 cggccggcgg ctttttcgcc ttgatcccgg cctcctcggg cctttcggtc aacgcctcgg
    18121 cgcctccgtc gaaatcttcc aggaagacca gggccgtccg ggcgaaggct cggcaccgct
    18181 ccggcagggc ggcgaaggcc gaggacgtct cggggtccag gtccggggcg gccctcgtct
    18241 ccgcggcggt gcccgcctcg gccttgcccg gcttcccggg cttcgatttc gacgcgggtg
    18301 cccccggcac cgggccggct ttctccgagg tgccgggggg gagccgcagg gcggccagga
    18361 actccggcga gagcagggcc gcgaaggccg cgtagagctc cgccaggtcg atgtcctgga
    18421 gggccgcgga caggtccggc gccccggccc cgcccagtct gcggcagagg gtgccgtagc
    18481 ggcccgtcgc gtcgtcctcg acctcgtgga cgtccaggaa gacctggctc tcgtagccgt
    18541 tcagggccag gtgcaggccc ttggcggcca cgtccttgga gggacgcagg taccagagct
    18601 cgctccgctg ttcccggaag accaggaagc ggccctcctt gccggaaagc cccagggcct
    18661 cggccaggga tttctggacg tggcgcttgc ctccgtccgg aaggatctcg gcgtagtagg
    18721 cggagtcctt gacccatccc gaggcccgcc cgtaggcgtt gttataggcc accagggccc
    18781 gctcgccccc ggccccgttc gagtacacga agacgttctg gttgaccgtt ccgtgctcgt
    18841 cccagaggtc gtagagcagg aagtcgtcca ctccggagaa gaggtggcgc cgcttcagga
    18901 tcgggaagat ctcccgctcg tgccgggcca cgaggcccga atcgggggcc tcgtcccggt
    18961 aggcgcggcg gtactccatc ccgtacttct cctcgaagcc ctcgacctgg ccgtgcccga
    19021 acatcggcag gcccggcagg gtggccatca gggtgcagac cccgaagtac ttgtccccct
    19081 tgccgaactg ggccaccgcg gtctcctcgt ccgggttgtt catgaagttg acgaatcgct
    19141 tgaggatgcg cttatcgaac tcctgggtgt tcttgatggt ttccttgaac ttcgcgttct
    19201 cttccttctt gagcatgttc atgaaggccg agttgtacac ccggtgcatg cccagggtcc
    19261 ggacgaagta gccctccatc atccagaagg cttcggccag gaggagggtg tccggggcgt
    19321 cccgggcgct tcggtccacc acctggcgcc agaactcctc gggcatccgc acgtcgaagt
    19381 ccttgcgcga cagggcgtgt tccgcccggg aggctatgtc ccccccggat ccgggttcgg
    19441 ggtaccagag gcggcggaag tgccgcttgg ccaggaccat ggccgcgtcg aagcggatga
    19501 tggggaaggc ccgggccacg tggaggatcc gctcttccac cgcctcccgg gcttcgggct
    19561 tgaggaagtc gatctgggcg gtgtcgttcc agggcatccc ggtcccgtcg ttgccgtggt
    19621 agatgtaccg ggtgtccccg gtacggaagt ccacgcgctt gaagaccacc gccgcgtcgg
    19681 agcggttgta gtagtggtcc tccagccaga tccccacgga ggggtcggcg gaaaggttct
    19741 ccccgttgaa tgtgtagccc gggaaggggg gatgccccgc ctggacgaag aggtccggcc
    19801 ggtgccgcac ccactcggaa tcgatgcccg tgtggttggg gaccatgtcc gcggccaggc
    19861 ggatgccccg ccaggcgcag cgctcccgga gttggtgcag ggaggaccag ccccccagct
    19921 ccccggcgat ctcgtagtcg aagagactgt aggcgctggc cgcggcctcg ggattcccgc
    19981 agagctgttt aatgcggcgg ctggcgtcgg aacgctccca caggccgatg agccagaggg
    20041 cgttgaagcc ccgggaggcc aggaggtcca gctcctcgtc ggggatgagg tccaggcggt
    20101 ctatcgtccg gccgtaggtc accgacagct ggtgcagcca caccaggacg ctcttggcca
    20161 gcatgacgac ccggggcatc cagtccatgt cctcggtgaa gcgctcgtac tcgtgctcca
    20221 tgccctcgta gacgtagacc cgggagggcc cggggcccgg gaagaagggc ttttcctcct
    20281 ccttgatcat gtccaggccg cccaggatcc gggccaggag gggccccagg aggaggcccc
    20341 agttccggcg catgtagtcc agctgaccgg gcagggaata gggcgaggcc tccacgggac
    20401 agcggaggag cttgaccagg gggcgttttt ccgggccgaa gaccggcagc ccgtcgaaga
    20461 aggtctccag gctttccagg accggcaggt acttcgtcgc gccccggaag gccgtatcgt
    20521 cgaagagttc cttgaagggt tcgaaggcgg ggttgtcgtt ggccagcttg aggaggagga
    20581 gttcctccag ggccaggacc ctgttgggta ccccttcggt cgtcccggcg agccaggcgc
    20641 cttcgtccgc cttgccccgg tacacgtccc gaggcgggaa ttccttggtg aaggccagga
    20701 ggagggcgtc ggtctgggcc ttgcccacgg ccttctccac ggcggccagg gcccggacga
    20761 aggcgtcggg ggccacggat tcccggaact tggcgcagac gaagtggagg atctcgtcca
    20821 ccagccccat ggcgttcagg cgcccggcct tcaccgagcg ttccgggtgg gcctgtacgg
    20881 agggccgggc gttcagggat cgggccagga cccgggaggc gtagaagttc gggaagacga
    20941 cgttgcccga gagggagaac agggattcct cgagcttgtg ctccgcccgc actgtccggc
    21001 gcacgtggaa ctccatctcc ggaccgtggg gccgggccgc ctccgtatcc gcgccttcga
    21061 aggccgagag ccgcaggact ataccgtagc gctgggtcat ggcgttcccc ccgtctccga
    21121 tcatagccga aggggggcgg ggtgtccaat cccgcgatgt cgagcctcgc tcgacatcgc
    21181 ggcgccgagc ctcgctcggc gaagttccgg tgacgtcggg cgggacagat ccgtccaatt
    21241 cgtccatttt ttaccttgac agttctaata tatcaggtga agatactttt acgtcacatg
    21301 gcctattctc ctcaaagaaa catcgtaggg gggtactgcc atgtccggca cgggaatcct
    21361 gggattcccg gacactttca acgggggact tcgcgtccca tggaggcttc gatgcggaaa
    21421 atcgccgccg tcatcctcgc gctcgccctg atcgcatccg cgggcgccca ggacaaggtc
    21481 atcaagatcg ggaccctctt ccccatgacg ggtcccgtcg ccaccgccgg ccagaggtgc
    21541 caggcggcgg tcgagaccat cgtggacatc atcaacggcg tgtacccgga cctggacctg
    21601 cccatcgcca agaaggcggg actcctggac ggatacagga tcgagctcgt gaaggcggac
    21661 caccagggca agcccgacgt ggccaagtcc gaggccgagc gcctctacgg gcaggagggc
    21721 gttttcgccg tcatcggctc ctacaactcc tccgcctcca agcccgccag cgccgtggcc
    21781 gagcgcctcc agaagatctt catgtgcggc gcctcctcct ccgcggccct caccgagcgg
    21841 gacctgaagt atttcttccg cctggccccc acggaccgga tcgaggccat cgagttcgtg
    21901 cactggttcg acgagctcaa caagtccaag aaggccggga tcaagaccgt cggcgtcatc
    21961 tacgagaaca ccgagttcgg caagcacgcc gcggacgagg ccaaggccca cgcccagaag
    22021 gccggctaca aggtcgtggc ggacgtcccc ttcacggtcg gagccaccaa cctgaactcc
    22081 gagatccaga aactcaaggc cgccaacccc gacgccctct tcggcgcggt cctgggcgcg
    22141 gactactccc tgatggtccg gaccatgaag cagttcaagt ggaagccgaa gatcgccatc
    22201 aacttctgct ccggctacca ggaccagaag atcaacgagc agctgggcaa ggacggctgg
    22261 ggcttcatgg gttccgcggg ctacaccccc gagttcggct cccggttcat gaagggcgtc
    22321 gcccccgtgg aggcgtactt catgaagaag gtcggggtgc ccttcgactc caactccatc
    22381 caggaagccg tggccatgta cgtcctggcc ctggccatcg agaaggccgg caccctggac
    22441 accgagaagg tcgccgaggt cctgcgcacc gccaccttcg acagccccct gtccatgggc
    22501 ggcaaggtca agttcgagtc ccccggcgga cagaacatca tggcccgaag cgtgatctcc
    22561 cagctccagg acggggccta caagaccgtg ttcccctacg agtacaagga cgcggctccg
    22621 atctatccga tcccgaagtg gtaatctgag gtaccggggg ccggccgaag gccggcccca
    22681 tttttccgcg caaccatcct atccggcgag gttcacggac tatgtcgtta ttctttcagg
    22741 tcatactgga cggcctatgg gccggtctgc tctacggcct catcgccgcg ggcttgagcc
    22801 tcatctgggg cgtcatggac gtgatcaact tcgcccacgg cgacttcctc atggtgggca
    22861 tgtacgtctc cttcttcctg ggcttcctct tcaaggcgga tcccctggtg tcctgggtcg
    22921 cctccgggat cttcctcttc ctgttcggga tgctgaccta caagctggtc atctcccgga
    22981 tcctgggcaa ggccatggcg cccttgctgg ccaccttcgg gctttccatg atggtgaaga
    23041 acctcgtcct caaccgcttc tccccgaact tccggatcct ggaatccacc atcctggagg
    23101 gacggtcctt catggtcggg gacgtgatcg tgcccctgcc ccagttcgtg acctccctct
    23161 tcgccctggc ggtcctggga ctcctctact ggatgctgac ccggacccgg ttcggctggg
    23221 ccgtccaggc cacggccctg gacggggagg ccgcccggct catgggggtc aacaccgaga
    23281 agatcttcct cctggtcttc ggcatcggcg gggcctgcgt ggggatcgcg ggagggctcc
    23341 tgccctccta cctggccacg cacccggacg tgggaaccct cttcagcctg atcgccttca
    23401 tgatcgtggc catgggcggg ttcggaagcg tgcccggagc cctggtggcc gcggtcatgg
    23461 tgggcctcat cgagtccctg gcgggcttct acatcggccc ggtctacaag tacatcgcgg
    23521 tcttcgggat ctacctcgtc gtcatcctca tccggcccaa gggcctcttc gggtggtgag
    23581 ccatggacta caagaaactg ctgtccaagt tcggttggcc cctcctgctg gtagccctgg
    23641 ccgccctgcc cctggcgggc ctggtgggac ccgggttctc ctcccacgtc ctgatcaccg
    23701 tccttctgac agcggccatg gcccaggcct ggaacctgat cggcggctac ggcgggcagg
    23761 tctccttcgg gcactcggtc ttcttcggga tcggggccta cggagcggcc ttcgcggtct
    23821 gcgtgggcaa gaccagcccc tggccgggca tgatcgtcgg ggccgtcctg gcggcggtca
    23881 ccgccgtgat cgtcagctat ccctgcttca agctcaaggg acactacttc gccatcgcca
    23941 ccttcgcggt ggtggagatc ttcaaccggg tcttcatgat ctgggactgg gtgggcgggg
    24001 ccgtgggctt ggactacacg atcctggagg acgggtggcg caacttcacc tggtccgtgg
    24061 acaagcgcgg ttactacctg ggtgccctgg tcctcttcgc cctggtcttc acgatcaccc
    24121 gggtcatcga gcgttcccgg ttcggctact acctccgggc cgtccgggag gggcaggaga
    24181 ccgccgaatc cctgggggtc aactccacgg gggtcaagct gacggccatg gccctatccg
    24241 ccttcctggc ggccctctgc ggggccttct tcgcccagta caacttccgg gtggacccgc
    24301 ccatggtgat gagcctggac atgtccatga aattcgtcct cgtcgccatc ctgggcggca
    24361 tcggtacctt ctggggaccc ctcctggggg ccctggtgct catccccctg caggagtaca
    24421 gccgggccta cctggccaag ctcggctcgg gggtcgacct gatcatcttc gggctcatca
    24481 tcatcctcat gatggtctac cagcccggcg gcatcctggg aatcctgacc ggttcccgca
    24541 agcgccaggc ccggctccgg gcgcgcctgg ccgcggaagg aggcgcgaaa tgagcgagat
    24601 gctgcaggtt cggggcgtct ccatgaagtt cggcgccctc ctggccaaca aggacgtgtc
    24661 cttcgacgtg gaggaggggg ccatcgtcgg cctcatcggc ccgaacgggg cgggaaagac
    24721 caccctgttc aactgcgtgg ccggattcta caaaccctcc gcgggttcca tccggttccg
    24781 gggcgaggag atcgcgggcc tgccctcgta caaggtggcc cgcaaaggcc tggcccggac
    24841 cttccaggtc gtgcggcccc tcaaggagat gtcggtcttc gagaacatcc tggtcggggc
    24901 ctacctcggc cacccggaca acaagcacgc ccggaaggtc gccgaggagt gcctggagat
    24961 ctgcttcctc caggaatggc gggactggcc ggcgggggac atgaccatcg gcaacaagaa
    25021 gcgcctggaa gtcgcccgcg ccctggccac ctcgcccagg atgatcctcc tggacgaggc
    25081 ggtcgcgggg ctcaccagca ccgaggtgcg ggagatggtg gaggtgatca agcgcctcaa
    25141 ggccaacggg ctcacggtcc tcatggtgga gcacatcatg gaggccatca tgcccatcgc
    25201 ggacaagatc gtcgtgctgg cctcggggga gaagatcgcg gaaggggccc cgaaggacat
    25261 cgtccgggat cccaaggtga tcaccgccta cctcggcaag aagtacgcgg agaccatgga
    25321 caagggggcc ggggcatgag cggcgaaatc cttctggaag tcaaggacat ccgctgcggg
    25381 tacgacgggg ttcccgtgat ccacggagta tccctggagg tccgatccgg cgagatcgtc
    25441 gccatcatcg gggccaacgg cgccggcaaa tccaccacca tgcggaccat tgccggactc
    25501 atgcatcccc tttcgggcgt catccgcttc gacggagcgg acatcaccag gaccccggcc
    25561 tactcgatcg tggcccgggg cctggcctac gtgcccgagg cgcggcggct cttctccaag
    25621 ctctcggtgc gccagaacct ggagatgggg gccttcacgg tcaaggaccg gggcgagatc
    25681 gccgcccgcc tggaccgggc cttccagctc ttcccggtgc tgaaggagcg ggccggccag
    25741 atcgcggaga ccatgtccgg gggcgaacag cagatgtgcg ccatagcccg gggcctcatg
    25801 tcgaacccca agctgatcat gctggacgag ctctccctgg gactccagcc gagcatcgtg
    25861 gagaaggtac tggagacggt cgtggagatc cgcaatcagg gtatcaccgt cctcctggtc
    25921 gagcagttgg tccaggaagc cctggagatc tcggaccggg gctacgtcat ccagaccggg
    25981 gccatcgtcc accagggcac cgcctcggag ctcctggcgt ccccggaagt gcggaaggcg
    26041 tacatgggga tgtagcgtca ttctgttggg ccgcgctgcg gcccaacaga atgacggcaa
    26101 cgaagccctt cgggcttcgt tgatttacag taggcgcggc gctgaagccc caatgggggc
    26161 ttcgtcgatt atcagtaggc gcggcaacga agcccccgtt tgggggcttc ggcgtttttg
    26221 ggtgtgcctt accttcccgc aacccccgcc ctcgtgatac cccacacgat cccgtacaac
    26281 accggcagat gaatcaggta gatcgccagg gatcggcgtc cgagccaggc caaagcccgg
    26341 atcgggggca catccaggaa tgttcggggc ggctcgaagc tgcgccggcc ccgggggtag
    26401 aggagggcgg cgacggcgat cccgaagagg aaccaggcgt accacggcag gaccgggagg
    26461 tagtccgcgg ggtggtagcc ctcggggcgc aaccccagcc aggccagggc cgggcccggg
    26521 aacctccggg accccagggc cagacccccg gccaggacgg cggcgcccag ggcggcggcg
    26581 gccgcgggcc ggtccgccag ggggtacgag acaagggtgg cgaaggctat caggtggatg
    26641 accccgaaga agacgaaggt cccggggccc agggcgagat aggtgaccgc cgagatgccc
    26701 gcggccacgg ccgccagacg cagcccccga gccaggaagg gccgtagacc ggcgcccgtc
    26761 ctggccttcc gcagggccat cgagagcccg gccagggtca cgaaggccgc cgcgatgcac
    26821 cgggggaacc accaccagaa gccctcccag aaggccaggc ccgcgacgcc gaagatcacc
    26881 aggtcgtagg caagatggta gacggccatg cagaccaggg cgaacccgcg caaggcgtcg
    26941 atctcgggat atcgttccat ggggggagta tacgacgggg gaaccgggcc ggacaggccc
    27001 ctttttcccg gtgccgtctt atcaaaggag aataccttgg tatatagtac gtgacaggag
    27061 ggccccggtt tgtcaatcgt cgtagagcac gagaagcgca agaaggagat cctggagcgc
    27121 gctttggacg tcttcacgga cgagggatac gaggatacca ccttccggaa gatcgccgag
    27181 cgctgcggga tcacccgtac catcctgtac ctctatttca agaacaaacg cgagatcttt
    27241 tcctacagca tcaagcagtt caccgtgggg ttggaggcgg acgtccggga gttcgcgacc
    27301 cgggaggacc tctccaacgc ggagaagatc tccgccatcc tggacatggt cctggcccgc
    27361 tgtgccgagg agaagcggct cctgaccgtc atcttcggct acctggacca cctgcgacgt
    27421 tccgggggag acccggacga gagggtccgg cgccggacca tccgcatgcg gcacatcctg
    27481 tccacgctcc tgatcgccgg catccaggcc ggggaattcc ggaaggttcc cgtccgggcc
    27541 gccacggacc tcctctattc cctgatcgag gcggcgatct tccggatcgt catcctgggc
    27601 aagtccgagg cgggtacctt ggtcgactcg gggcacctgg tcgtggaggg gatgaaacgg
    27661 tgaccggccc gaagcccgcc tggctccggg tgcccatccc ctcgggcccc gaatggagga
    27721 acatgagccg gctcctggac cggcggggcc tccacacggt ctgcgattcc gcccgctgtc
    27781 cgaacaaggc cgagtgctgg ggctgcggga ccgcgacctt catgatcctg gggaacacct
    27841 gcacccggaa ctgccggttc tgcgccgtcg cgaccggtcg gaccggggat tcggtcaacc
    27901 ccgacgagcc gggccaactg gcccaggccg tcgcggaact tcgcctgacc tacgcggtcc
    27961 tgaccagcgt ggatcgggac gacctgccgg accggggtgc gggacacttc gccgcctgcg
    28021 tccgggcgat caaggcgggc aacccgggcg tccgggtgga ggtcctggtg cccgacttcc
    28081 gggagggtga gatcgacctc gtcctggccg cgggccccga cgcggtggcc cacaacgtag
    28141 agaccgtgcc ccggctccag cgggtccggg acgcccgggc ctcctacgaa ggttccctcc
    28201 ataccctgga acgggccgcc gccgcgggga tcgaggtggt caagtccagc ctgctcctgg
    28261 ggctcgggga gacccgggag gaggtgctcg gagtcctgga cgacctgcgc cggatcggct
    28321 gcggcagcgt cgtcctgggc cagtacctcc gaccgacgcc ctcccaggtt cccgtggcgg
    28381 agtacgtgac cccggagacc ttcgccgagt acgcccggga agcccgtgcc cggggattct
    28441 ccgccgtggt gtccggtccc ctggcccgga ccagctacca cgcccgccag gccttcgagg
    28501 aggtgtcgtg accctccgca ggatcgtgaa gatccccgcc gggaagctcc tcaagctgac
    28561 cgcggacgtc gagaacggcg tcctgaagtc cgtcgccgtc cggggggact tcttcgccca
    28621 cccggaggag ggcttcgacc gggcggagga gagcctggta gatatccccg tcggggagtt
    28681 ccggagcgcc ttttccgccg ccctcgcccg ggagggcgtg gaactcttcg gcctctccgc
    28741 ggacgcggcg gccgacgtct tcgaggagat gctccatgac cttccgcctg cttgaaaccg
    28801 gggtctcccc gggagccttc aacatgggcc tggacgaggc gatcctgggc gccgtggcgg
    28861 agggccgcca gcctcccacc ctgcgcctgt acggatggaa tccccccacc gtgaccctgg
    28921 gatacttcca gggactggac gaggaggtgg accgggaggc ctgcgcccgg atgggcgtgg
    28981 atgtcgtccg gcggatcacc gggggcgggg cggtcttcca cgacgcggag ctgacctata
    29041 gcatcgtcct gcccgagagc catccccggg cccggccggg catcctggac tcctaccggg
    29101 ttctctgcgc gggggtcgtg gaaggcttgg cccggctcgg cctgtccggg gagttcgcgc
    29161 ccatcaacga cgtcctcgtg gggggacgga aggtctccgg aaacgcccag acccggaagc
    29221 ggggctgcat cctccagcac gggaccatcc tcctggacgt ggacgtggac cggatgttcg
    29281 cccttctcaa ggtgccctcg gagaagatga aggggaagct gatcgaggac gtgaagcaga
    29341 gggtcacgag cctgcgtcac cagctcggcc ggagcgtggg ccttcccgag gcggccgccg
    29401 ccttcgccgc ggggttcgcc gccgccctgg acgtcgtcct ggaccccgcg cagccctccc
    29461 cggaggagct cgaggagggc cgccgtattg cacgggagaa gtacggcacc gaggcctgga
    29521 cagggagaag gaagtgaatg ggtgaatagt gaatcgtgaa taggacggtc ggggtaatag
    29581 ctatgaactc catcggccgg ctcttcaccc attcacccat tcacttattc acctattcac
    29641 ctattcacct actttcattc caccttcgtc ccccgcgtcc cgtccttcgc cgccgcgatt
    29701 ctccgggcca gcacctgggc ctcgaagagg gcctccaaat tcgaggcgta cttggtgatc
    29761 cgggagatgt aatcgagggt tttccggggc gtgccgccct tggatacccg gccgtacccg
    29821 gcgttgtaga tggccagggc cgccacttcg ttcccgccct catccaggca gtaggccaga
    29881 tgggcgatcc ccttcttcgc gttcaggacg gggtcgtaga actgctcgat ggtcatgttc
    29941 gggaaggact tggaattaag ctggaagagt cctcggtcca cgctctcggc gttgcggttg
    30001 atcgcccggg ggtggaactg gctctcctcg aaggccaggg cgaaggccag ggagacgggg
    30061 accgagtact cgacggcgtt gtccaggatg gcctgggcga tctcggtgga cttggtgatg
    30121 ctggagaaga aggccaggac cgattcccgg tacagggggt tcccgtagcc cgcggcgatg
    30181 aaatcctcgg gggcgtggct ccggaaggcc tggacgtact cggtctcgtc gtaggaggag
    30241 aacttacgga actcctcggg gacgtaggct tccggttcca cggcggtcac ggaggacatg
    30301 caggacgata cgacgaccgt gagggaagcg gcggccaacg ccgcggccag gaccgccaaa
    30361 ctgcgaaaca gggttcgaga agtgggaacg ggcatgcgat cgatgctcct gacgatttgg
    30421 aatcgggccc gaggggaccc gctgcatttc gaaatagggc cggccggggg gccggaggaa
    30481 tcggggcccg gaggcccgcg cgacgggtcc ccattttatg gagatttcgg agatcgtcaa
    30541 gccccgcccg cgggagcccg gcgcccttgc gcccgggacc cgggcatcgt acactggggc
    30601 ccggaggaga tatgctgaac cgcgacgaag ccatccggct ctggaggacc cacaatcagg
    30661 acgaatccct gttccggcac gccctttccg tggaggcggc cctccggcgg ttcgcggtcc
    30721 ggtacgggca ggacccggac tggtggggcc tggtgggact cctgcacgac atcgactacg
    30781 agcgctggcc cgaggagcac ctgaagcatg cccggaccct cctggccggg gcgggcctcc
    30841 cggaggactt catccgggcc gtggagagcc acggctgggg cctttgcacc gacgtggagc
    30901 cgaaatcgga catggagaag gtcctgtacg ccgtggacga gctcacgggc ttcatcgccg
    30961 cctgcgcctt cgtccggccc tcccggagcg tcctggacat ggaggtgaag agcgtccgga
    31021 agaagtgggg cgccgcggcc ttcgccgcca aggtggaccg gtccgtgatc gagaagggag
    31081 ccgcgatgct cggcctctcc ctggacgagc tcatcggcga gaccatcctg gccctccggg
    31141 acgcctcgga ggcggtgggc cttaaaggaa ccttgtgatc ccgggtcact ccgggcgcta
    31201 ggagtttgtc gggcccgccc acgaaaacca aaggaaaatc gcatacttgc gattttccta
    31261 cctcgcaaac tcccaaagga gcccgataat cgggactttt tacgcctact ggcgtaaaaa
    31321 gtccacaaat ccgacaggct cctagacgcg cccgcccggg acgggacata ctcgcatccg
    31381 ccgcggccgt cgcg
//
LOCUS       DAOTEI010000019        30883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_975_length_30883_cov_4.662839, whole genome shotgun sequence.
ACCESSION   DAOTEI010000019 DAOTEI010000000
VERSION     DAOTEI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 30883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30883
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_30883_cov_4.662839"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="P5313_03140"
     CDS             complement(<1..399)
                     /locus_tag="P5313_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY79392.1"
                     /translation="MKRKCILTAVLLAAAFAAWGQGASAWAEVEFAVGTDVTILRDNK
                     PLPADELLGFRLEQGDQVQTGKGTFLELRLKPSGGVLKLSENTVFVLTTLSESPERGF
                     KLVYGRVRAKVDKLARGSSFNIASDSAAAGV"
     gene            484..1443
                     /locus_tag="P5313_03145"
     CDS             484..1443
                     /locus_tag="P5313_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79393.1"
                     /translation="MESILNEIRLAFSDPQINNLAASAPAEARDSLYSMRFGQSEEYF
                     LELARDIEIPHFPIHHDVRLREPSETYLQALRATLRQLVSLLPATFRDLVHLFEPAET
                     LKPAFFHLYKVQESVYLYVLRIDLLNRPLETDLVEAGTNETTPAYRTRRLYLESELIP
                     LTGIEREEGTIRAFRVRQMVSNTWIGESGKGYQVRGIWMDADLSKFFSKLFLPEGKRV
                     YPFLPFFCKYKTVCSFVPVPEGTSRKRALPVLHRALGFLEPEMERIQGILKGSTFSES
                     DERFLALRARIPPSWKDLYREVSVRPYLNPRDMKEYELEYPRS"
     gene            1424..2839
                     /gene="murD"
                     /locus_tag="P5313_03150"
     CDS             1424..2839
                     /gene="murD"
                     /locus_tag="P5313_03150"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRY79394.1"
                     /translation="MSIRDPEARDLSGRRVTVMGLGLNGGGLASARFFALRGASVTVT
                     DMKDETALAESLAALEGLPIRYVLGHHELEDFSGADLVIKNPAVRADSPFLSAARAVE
                     TDISTFLRYCPGPVAAVTGSKGKSTTASALAHALSAAGTAFLGGNITVSPLTFLDQVR
                     VDTPVVLELSSWQLGDLKGRGLLRPRVAVLTRIVPDHLDRYPSMEAYVADKRLIYADQ
                     DPSCYTVCDRDDPYGRSFGTETPGTARWYADTLEAGLSGAWFEGPVGLYRDPDGRTEE
                     ILPERLRVPGPHARRNLLAAALGARLMGAPAGTILRALGEFPGVEHRLEMFLDAGGVR
                     YCNDSAATVPQAVEAALHAFDTPTVLITGGTDKNLDFSPVRAAYRRAKAIVLLAGTGS
                     EKLRTLLDQDGTSYEGPYDDLEKAVRAAAAKTIPGDTVLLSPGCTSFGMFRNEFDRGR
                     KFKECAARIAGVPVTENRGSE"
     gene            2836..3228
                     /locus_tag="P5313_03155"
     CDS             2836..3228
                     /locus_tag="P5313_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRY79395.1"
                     /translation="MRILALEGDYSVYRFDPDRDIPGSLLAGSGFRSVTRTDQELSVV
                     ARTGALRGAPREEAGWTLWMVQGPLDFGLLGVLSSITGPLAAAGVSVFAVSTFDTDYI
                     LWKKDRAGEAVRALREAGFEVEGSARFL"
     gene            complement(3245..4075)
                     /gene="amrB"
                     /locus_tag="P5313_03160"
     CDS             complement(3245..4075)
                     /gene="amrB"
                     /locus_tag="P5313_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRY79396.1"
                     /translation="MRIRNRSLEGGWYPGDEEGVRAAVDEWERTLEADPDRTAGAGVA
                     PHAGWYFSGRLAWDTLRRARTDAETVIVAGGHRPPGSAPLAAEEEAFRTPLGLVPADR
                     ELAEALGAYAPLEADRWADNTVEVLLPLVRLRFPEARVLWLRVPNDDSAENLGAAAAR
                     AAANLGRKTFFLASTDLTHYGPSYSFCPKGRGPEAQEWVRDVNDRGFLDALLALDSRD
                     VIRRGNEDRAACSPGAAAAAIGFARALGFTNPKLCGYYTSRDVQASESFVGYGSVVFE
                     "
     gene            4142..5395
                     /locus_tag="P5313_03165"
     CDS             4142..5395
                     /locus_tag="P5313_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79397.1"
                     /translation="MDGSKRTLFLVASLAVFLAALGVSSVRLALRYSENLRKSQSVFE
                     TLSSAASRTLAQTGPQDEAWRTLVRTVYRNDDSVLLVVLHAPGTGILHMMPKSAPYLD
                     GSPQTAPVPPELYYPPQTVSRLSAVVGSAAGSPVTLELLYSLARQRDVYEAARLGLIL
                     AAAWTALCLLSATAATLRSGSRTPAAVPASVSGGPAPGPVVPGPVLPDEDFQLPEIPP
                     NPDGAADRGESGPSAPSRESPPPREPSGHPRGLYSPDSGLGWPEYLPERLGAELSRAA
                     SFEQDLVLMELSHEGISRGSPAYRVIIRILLEFFSFRDLAFEQDPAGLAVILPNIDAE
                     HALRMAEEFLKKVTSILREEGASSDYRKIFIGLSSRSGRLVDSERVITEARAALTRAR
                     EEGDSRIVAFKADPNKYRDYIAARP"
     gene            complement(5611..6576)
                     /locus_tag="P5313_03170"
     CDS             complement(5611..6576)
                     /locus_tag="P5313_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRY79398.1"
                     /translation="MTAFRRKAAVLSAVAGFLALLVAAGVAFSPGARNARASRIRLLV
                     PDPAEIHAVEVSGPDGSLVFRRFGAAWTLEEDGLPLPARTERIEAYLETLHSVRSLVE
                     VSRREASWDELGLDEGSSRRIRLLLRDGASAADFRVGRYAPDGSRVFLRKSGETRAYA
                     APAATASRLPAGRMAWLDLRVFGEPVPREDVQRLRVSGSVRFPDGTVRRADYVLERGL
                     SGAWTSPQIPDLDTAAAGRLVRSWMGAEAEDFSLRMPEDREGLRVDLELGGGSLRSLR
                     ISGTPDPEGGYTAALTGSGQYLILDARILRDLLMTPGELSAGSRP"
     gene            complement(6573..8207)
                     /locus_tag="P5313_03175"
     CDS             complement(6573..8207)
                     /locus_tag="P5313_03175"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HRY79399.1"
                     /translation="MKTSDTAARRLREEGILKGLTLLVLALGAAAGSLYRIRIDLTED
                     RAFTLSPASRSLAARIPDTVNLTYYRSAALADLHPGIRMAQDLLSEMESVSQGRIRVS
                     VRDASEDPGRLESLGILPRQLQVEGGGEARIATVYAGVVAEYLDDIRVLPSVLGPERL
                     EYEVVKAVLGLVGGTVPVAALREGDGNRDPEEDFRLLRAALARAGYEVRLSPPGSEVE
                     PDVSVLLVLGNAALDERDAYSIDRYLAGGGRVLAAVRGVRVDPDRNLEARALGADPVL
                     DLLAAYGVRVRRELVLDASSLSVPFQVQGSGGETTYRYLRYPHWVAVERENVTVSHPA
                     AAGFSGLDLYWPSPLELSDRPGLSSEALVKSSTRAWKQTRAFAAAPGEESLYREEEDA
                     TRGQYVLAAALSGTFPRLYGDAVPGLAGRPGPDGRLAPAPAPTSPNAGRPGRLVVAGS
                     SDFLTDLAGMSGSESNILFAVSAADWLSAGDDLPLPSAARGPRRLRLPEEEEVRRRAV
                     GAAYAVNILVVPALVGLAAFLRASRRTRREREGRAA"
     gene            complement(8204..8926)
                     /locus_tag="P5313_03180"
     CDS             complement(8204..8926)
                     /locus_tag="P5313_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY79400.1"
                     /translation="MSPVLAVFRRELRSFRNTAPAYIFAAFFLAVSAAWLFLVRGFFV
                     RGEASLRPYFEVMPALFAVLLPALTLRSWAEERRTGTYEILVTLPLRPGDLVVGKHLA
                     VMAVVGAVLGLSLSVPLTLSRYGDFDAGVLVTEYLGALALASACAALGQYVSSRSRSP
                     AGAYLATSALLLALSAVHRVPAFLTLPGFLARVLVWISPASHFESFVRGVLDSKDLVY
                     FGILTSGALYLSAKALVREAWR"
     gene            complement(8923..9870)
                     /locus_tag="P5313_03185"
     CDS             complement(8923..9870)
                     /locus_tag="P5313_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79401.1"
                     /translation="MIEVRDLRKSYGEFEAVRGVSFDAGPGGVVGILGPNGAGKTTIL
                     RILAGYHGPTSGTARVAGIDAAEDSRELKRRVGYLPENAPLYPELTAEEHLAFAAEAR
                     GLRGPAKARAIREAAERAGAAEARAIPAERLSKGWRQRVGLALALLGDPPVLVLDEPT
                     AGLDPNQIRQTRQLIRDLGRDRTVLLSTHILGEAEALCSEILVLFRGALAGKGSPREI
                     SEGLASRDRLEAVIKAPGAPEVEAALARLAGAGLEGPPSPEGVGAWRVRLGFPAGAAD
                     PAESVSDWAASEGFKLLELKKERLSMEEAFARLTGEEGE"
     gene            complement(9880..10809)
                     /locus_tag="P5313_03190"
     CDS             complement(9880..10809)
                     /locus_tag="P5313_03190"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRY79402.1"
                     /translation="MNLEAFILGTGGMMPLPHRHLTSVLLRREGDLFLFDAGEGTQVS
                     LRRLNLRWKKINAIFISHTHADHVTGLPGILMLSSQVDRNEPLYIYGPPRIAEYVESS
                     RRVLDMYINYEIVVREIEAPCLCHEGEGFRIRAFPLRHTKPCVGYVLEEDPRPGAFFP
                     EKAEASGVPRGPLWSVLQGGSPVDLPDGRVVRPEEVMGAPRSGRKFGYVTDTLYFPEI
                     APNVSGSDLLVCEGMFERELLASAQEKRHMTAEQAARIALDAGGVKKLALIHYSPRYT
                     EQNLKRLLEEAREIFPDTVLSRDRMCIPIEYLD"
     gene            10898..12388
                     /locus_tag="P5313_03195"
     CDS             10898..12388
                     /locus_tag="P5313_03195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY79403.1"
                     /translation="MVEFQSLGIREDLARALASFGFEGPTEIQRRTVPALLDRSDAFV
                     SSSTGTGKTFGYLVPLLQLAPGETRKPFALVVAPTHELAVQVEKELDRLAAAAGIRAD
                     TVLLIGSAQARRQAERLRKGGSFVIGTPGRVRDLLAARELDLSELSFLVLDEADRLFE
                     KEALAETADILDALPEACCRVLVSATLPDRVRDKAAPWFRAPEALVLDNSEVLRENID
                     HWVFHSSSRRKIDFLRRFESAVRPKRCLVFASSNAQIFNILRKLEYLEFPTAELRAQG
                     DKEWRKQALDDFREGRVRYLVTSDLGARGMDVKGIDYVLCMDLPEEPTVYMHRAGRTG
                     RAGERGVSVLVADIVELKRASKVAVRYGFSFQCRILEGGEVHEIDPENFFALAEEEEA
                     DRAVKPPLEAKRFSDMEGRRPRGRLSPSGGVPAGARSRKPRAGSPALRQETRRPDGPS
                     GAAETGPGRGHRPSQGRRGAPGASGPAGPGKSRPDGRRDGRRKRSG"
     gene            complement(12398..14134)
                     /locus_tag="P5313_03200"
     CDS             complement(12398..14134)
                     /locus_tag="P5313_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRY79404.1"
                     /translation="MNREAVPREREYVTGQLIPYLGNKRSLTRHLGSLFRSLTEGRNH
                     PVFLDPFAGSGSVSRLARTLGMRVKANDWEPYAYALNRAWLVLRASDLPDLWAEEGGL
                     KAWLDRLNSFHPARDNLSPDPEPEPYMARWYAPRDTSRADWRVERLFYTRENAVFLDR
                     VREHIERAYPSGESGFPDSPASRRRDLLLALLVLEAAVHANTSGVFKAFHKGFGGHGR
                     DALPRILGAMELEHPLLPEAEPAEVFREDAAVFVRRWTADLAYLDPPYNQHQYGSNYH
                     ILNTLVRWDRDPVPLETGPDGRLLRKAGIPPAWKETRSPFCGRSTARAALAGLIDALD
                     ARTLVLSYNTEGLVSPEELYELLSGRWEVRPEVLDYVRYRGGRQSGTRRVRNHELVYV
                     CRARSGARGSVSGLARLRAELKLRRLFSSRFDPRKIPETFEPSPGEPGIATPLGNLRM
                     WRFHRFEDPLPDLGAVGVEDLTEFGGRLEACEVTDHARTVEVLAAVARDCGGRGPPLA
                     PSRDLADARRAAAEALGSLRKLAHPRYVREYERAEEILTELGEDLPVLGERLLSGLER
                     LRVQRAERLRNG"
     gene            complement(14124..14483)
                     /gene="queD"
                     /locus_tag="P5313_03205"
     CDS             complement(14124..14483)
                     /gene="queD"
                     /locus_tag="P5313_03205"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRY79405.1"
                     /translation="MYTVRVEADFSAAHFLSGYRGKCENLHGHNYRVRAFARGSDLDE
                     SGMLLDFGVFKKALREVCEGLDHRLLNENPAFLDSPSAERIARYIFDSLAARLPGTPL
                     QAVEVFESATSMARYEP"
     gene            complement(14549..15118)
                     /locus_tag="P5313_03210"
     CDS             complement(14549..15118)
                     /locus_tag="P5313_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79406.1"
                     /translation="MSRENLRSVLDYILNKADRDEFEAIVKACERRRRDLSLFASIGG
                     VNPERAARNTAQAVEGSMGASLDGIRSMIRDFVADIIRKNAPEVTEEQLEELLASYVP
                     DPGARGPAEVSPLPPDALAKMVKDFTDYSLGLMPPSAQQALWERMPRWQEEYWTAFPA
                     ELKAFVKAFLEGRMDADTFWSAVYSFLGL"
     gene            15266..16075
                     /locus_tag="P5313_03215"
     CDS             15266..16075
                     /locus_tag="P5313_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRY79407.1"
                     /translation="MSENAESEAGRHLLEPLGQVGSLMGEIPEPEIAFPGRSGPRDSR
                     GRLRLDVCLAVSLALHALVLALFPVPGTGGRGAAASTVRVNLILGTGAAAREAAGSVP
                     SGEGAAPAAPSPGAPDGSASDAPEDSLPRPGEAEPAGGEEPGAPDSAVSPSAPGAPAA
                     GSAAGSGGAGYPSPSDRAAALLLARVEAALVYPEAARRRGTEGVVGLRILLDGSGALA
                     DLKITRSSGSSLLDKAARETVAACLPVPNPSGIPLAFELAVRFTLKKAAAP"
     gene            complement(16129..18018)
                     /locus_tag="P5313_03220"
     CDS             complement(16129..18018)
                     /locus_tag="P5313_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY79408.1"
                     /translation="MRKAACFILVAALAVLGPGAQVRDLGEVSVTAESWSPAALAPGG
                     SVVVVTAEQIEASGAKNAAEALASVPGFHSLDYGNPGSVRAPSLRGASSGQVLVVLVG
                     ARLNDARQGNVDLGQIPAESIERIEVLKGGASSLYGADAVGGVIVITTKRGGDPRLTV
                     TAENKVYPAALGADLEYLAAAQRLGASLDTRIGAAGLSASGSFERAGDSFPAAQAGGA
                     VLERGNAGFLGGSGSLGLEVPVLGGILSAALSGRYEDFGVPGILTWLTPNAEQRNRDF
                     RGTAAWSSDALMRGRLSLDVSAFGAWSRMEYEDPDEPADDRHDTFSSGADFRFRYLAS
                     DAVELPAGLEFRYDGAESTKVGNPRRYHAGAYAAPVLAVGRGVKVSPAVRLDWYDDYP
                     AGLTYSLGASAAVSEVVTLKATAGRSYRAPAFNDLYWPADPWTEGNPDLLTETSWYGE
                     IGLETLGSRIETAVNLFARYSDDLIRWGDPDGDFVWRPENLAAAAFIGADAAVAVHAG
                     PVSVSASYGLLLTYDLADGRTLANDVRIANYPVHTVKLEPVYSDGFGKVSLRGSWRSE
                     RYQSPTITLDPVLLLGARVELRVAENADVYLDGENLLNRAYAEMDGYPMPGITLKAGL
                     RILLD"
     regulatory      complement(18229..18384)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(18491..19780)
                     /locus_tag="P5313_03225"
     CDS             complement(18491..19780)
                     /locus_tag="P5313_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY79409.1"
                     /translation="MGFDRNERIGILGAGAAGLSLALRLADSGFRSVTVLERESEPGG
                     KCRTFRAEGGNWELGAVMGTTDYRDTLDLMRRVGLDPWRSYAPDRTPEDAFVGRGFWP
                     CEKLFPGWIGIGELPEGILEILRYHILAPRYRAAFGPNHLGTPGELADSFQAWVDRHR
                     MGVLAKAISIPYTTFGYGYYDESPAAYVLKFFEPGIVRALALQFKFFKWREGVQTLWE
                     RLAESLDVRYSLDVREIRRGGTVAVSGRERSSGEPFRFEFDRLVLACPLDDSLGFLDA
                     TTEERELYTKIRHKDYRVYLKRARGFRVPAGFAPARFDRSGIGRAMIWDDRVPGSGIH
                     TFYVLGDGKQSDAEIEAGLDEDIRTLGGEPGETVSRVRWKYYPHVTCEDYRAGWYDRF
                     EALQGRIRTYACGEIASFSTVERVIRYSRDLSRRFFE"
     gene            19843..20142
                     /locus_tag="P5313_03230"
     CDS             19843..20142
                     /locus_tag="P5313_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRY79410.1"
                     /translation="MHELPIITRVLELSLAAAPEGSEICRIKLRVGSLCDAEPLWLQR
                     YFRIAARGTPARDAELAVRRVPAGSTEDAVRDPDSAFGYVLESIEIRDAPVGAVP"
     gene            20139..20852
                     /gene="hypB"
                     /locus_tag="P5313_03235"
     CDS             20139..20852
                     /gene="hypB"
                     /locus_tag="P5313_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRY79411.1"
                     /translation="MSGTSRIIDVRASILEDNGKNSEALRRSLSGRGVFLVNLMGSPG
                     SGKTSLLAALLPLLSPPLRPAVLEADLDSRVDADALEARGWKAVQLRTGGFCHLDAFM
                     ARRGLAELGVLPPEDGIPGVPNDAGPDSPFDIVFLENVGNLICTAQVDTGAHLNLALL
                     SVPEGDDKPLKYPVMFAKADAVVVTKTDYLDREDFDVEAVRRRVEGIRPDSRLFTVSA
                     RTGMGMPELARFLQEKIRT"
     gene            complement(20849..22072)
                     /locus_tag="P5313_03240"
     CDS             complement(20849..22072)
                     /locus_tag="P5313_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY79412.1"
                     /translation="MDTRKAYAELRRHLDTLPVGFPRSRAGGRILRRLFTPAEARVAL
                     GLSWKFRSPAEVETALKARGLDPRELAREAGAEAEPSGAPDGSGLASLLGALASKGSI
                     LRKATDGTYALLPFVVGMYEMQIARLSRDLVRDTVDFLHQGFGLELLTTGEPQTRVIP
                     IDAAIRPEHRIAEYDECRFLIRNAGGRIAVLDCICRRATDLAGKPCKSTDRRELCMVF
                     RDFADTVIREGWGRAVGEAEALELADLNQREGLVMRPSNEREPQFLCACCGDCCGLLS
                     VVKAMKRPADFVATNHRAVVLPGNCVSCGACARICPMEAIVLDGPAAKTGGRSGARIL
                     ESRCIGCGVCTAACKFEALRLEPLTPARVPPKNTEELLDRLAASRPGTLRKLRLGIAG
                     ILGLKTHPHGRRGRS"
     gene            22237..22761
                     /locus_tag="P5313_03245"
     CDS             22237..22761
                     /locus_tag="P5313_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79413.1"
                     /translation="MKKAIILAALAAMLAVPAFAQWRVDIGADMPFYAGITDSIVGDA
                     TGGNIPYFIPFPNAALSYQFDLGLISLGVGAKVYSFILLNILSPMAYVEMDFDPLVVN
                     LSAWGGGFLVFGLVNEFLTENFIVPDLSVMFKLGKRQTLRLGGGVMTALSLEGSQGVF
                     PFIAYGTIKFSFPL"
     gene            22846..24396
                     /locus_tag="P5313_03250"
     CDS             22846..24396
                     /locus_tag="P5313_03250"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRY79414.1"
                     /translation="MTSRLSDLLPFLPAHELAGPADVPVTGMAYDSRDVRPGYLFFAL
                     PGLHADGHDFIDRALDAGASAIVHSRDPGPFRQGIAYVRVPDSRFALSPAADAFYGHP
                     SRSLGVVGVTGTEGKSTTVYLIWQLLNLSGARAGFFSTVMSDTGSGELPNPEHQTTPE
                     ANAVHRMLADMRDAGYEYAVVEASSHGLSPRTNRLGDVAFDAGVMTNVTHEHLEFHGT
                     VEQYRHDKANLFRALDRVGHRKVIAGRSVEVPSFGVVNADDPVSDLFRRACGKPVYSF
                     GAADAPADAFARAVASDAGGSSFEYVEEGSSYPARIELPGEFNVRNALAALLTVSRLR
                     GIPASELLPFLPKLRPVRGRMTVVDRGQPFEVIVDYAHTPSSFQAILPPLRSRVRGRV
                     ICVFGSGGERDTEKRPLQGRIAAEYCDVVILSDEDPRGEDPMALLEDIAAGCPGLVRG
                     ERLFLIPDRRAAIRRAFSLAGPGDLVLLLGKGHENSIIYARGTIPWDEIAEAEAALAE
                     LDYERSSP"
     gene            24393..25478
                     /locus_tag="P5313_03255"
     CDS             24393..25478
                     /locus_tag="P5313_03255"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRY79415.1"
                     /translation="MNTVILYGGRSGEHEVSLASAASVLRHLPPSHEPTLIGIDRDGV
                     WYLQPPRAVEKARGGSGPLEILKDESRVAAAVPGRGLALLSGDPIPADVVLPVLHGTF
                     GEDGTVQGLLETAGLAYAGADVLGSAVAMDKEVSKALWARAGLPVPPFRTVRAADLEP
                     ARFEVLRSDLEASFGYPMFAKPACAGSSVGTSKIDNSAGLLPALRSALEWGSKALVEP
                     FLQARELECSVVGNGEPTAYPPGEIVSSHEFYDYEAKYVDPDGARLLIPAPVPAEISR
                     LVMDIAVRAYKLAALSGMARIDFFLDRKDGSVYLNEANTIPGFTHISMFPKMCEAGGL
                     PYPELLDLLARLGIERKRERDGIRYTR"
     gene            25691..26890
                     /gene="rocD"
                     /locus_tag="P5313_03260"
     CDS             25691..26890
                     /gene="rocD"
                     /locus_tag="P5313_03260"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRY79416.1"
                     /translation="MKTQEYIDLDEKYGAHNYHPLPVVLSKAKGVKVIDPEGKEYFDF
                     LSAYSAVNQGHLHPKIVKAVRDQLDRVTLTSRAFHNDKMGMFLKKLCEFTGYEMALPM
                     NTGAEAVETAIKAARRWGVEVKGVQNGKQEIICMEDNFHGRTLAAISMSSDPDSTVNY
                     GPYAPGFIRVPYQDIKAIEKAVTPNTVAVILEPIQGEAGVVVPPEGYLKAVRALCDKN
                     KMLFIADEVQTGFCRTGKRFAWEHENARPDIMCLGKALGGGVMPISAIVADHGVMKVF
                     TPGTHGSTFGGNPLAGAVGIASLEVLEEGKLEENAERLGRIFRDEVAKIPCKKMVLVR
                     GKGLLNAIVFEKGFEAWNVCLALRDAGLLAKQTHGNIIRFAPPLVINEKELREAIAII
                     RSVLEKI"
     gene            complement(26994..28226)
                     /locus_tag="P5313_03265"
     CDS             complement(26994..28226)
                     /locus_tag="P5313_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY79417.1"
                     /translation="MKRIRTVIFTFGTAAAALCIVFLVWAFVLRERSTRRLYAEYEVY
                     RALTALTDMYRDPGAVMPPDDRILGFGLYRSDGTRLYQHGRTPDRLAPEILQDPASAR
                     RFTDSSFILVRPFGMDPLRRPVPGRPDRPGSGMMMPMGGAARFAYIEYGLGSFRREQR
                     ALSAGALAITAALGAAYALLVHLYRRNLDLRDREYRNRELVQLGEAARTLAHEIKNPL
                     GVIRVQTALLARTLGGGSEEGFRIIDEEVRRLGGLVDRIRDFLRSGEGRPETVDLGEF
                     LEGWAKRHGGGVRLEGAVTEGAAVRIDRGRLTDILDNLARNARESMEGRTDLPVELAC
                     GVKRGQAVVSVLDRGPGVDPEEEARLFEPFHTTKEKGSGIGLALSAKWARTAGGTLSY
                     RPREGGGSVFELRLPVAK"
     gene            28328..30256
                     /locus_tag="P5313_03270"
     CDS             28328..30256
                     /locus_tag="P5313_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY79418.1"
                     /translation="MTNAEGSDLLRKALEAGSRRDYESAADILTRIVSETDEFPEALL
                     YLGRSLHSLGDFGKAIGAFRLYLRDTSAKPEGWFFLGRTYLAVSRPLESARCLRKALE
                     LGADGAETWSLLGLAALRLRKSKASVEALERAVTMAPGNPRVFRGYLNALFVHAVRTL
                     NRGDPDMARQMLSFVIANGLDGVPQRLWRSRAYRELRRIPEALADCRAALSENPKDPG
                     LLTLEALLLLADGSPEDAAAASTRLRSLWPDMDGVAWTEDSLERYRAVALLRDGDHAG
                     ALRAAVGILRREGPDAGMRALAAEACRELGRFDRAVEHLTRALELDPGNPDLLFERAV
                     CRWRLSEFQEALADFGRARRAGADADAVEYWSVLCRCRLGQGGPSVLESLQKMLRLRP
                     ADPPLMFALGEALYKDGRPDLARNWFDRTFQADPMHEMAALYRISSRESLGDREGAFE
                     AYADYLERWPDNSEIRREYVGALMAARRFADAAAGIEAGIPYEPPGNRDHAALAACYR
                     NIGRYREAGTLYRSLLRASPKNEDFLLGLALCLDKSGSGATAVELLEKGAPYVGTSGP
                     WSALGILYARRGRVERALEAFRKAADLDPRDPKPLRNLARIYEKTGLADLAKKYKARA
                     AAAERSSKTPESLAKPGR"
     gene            30285..30842
                     /locus_tag="P5313_03275"
     CDS             30285..30842
                     /locus_tag="P5313_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79419.1"
                     /translation="MSLKAMDLFDAYAKSQLPKDHGFIVSSFFSPNSAYSRYEVVSYN
                     NVKSIYPSGEGLTFQTDGKKLFILVEPQNYAKKSEEPYVRSSTEQIPLRFSELHCHTA
                     KNQTRVYYGKKAVLSYGAFTILKPTGINFSFCFYYLPDMFDSMRIFFEKTFNKEAGVP
                     LADAKKVAAAVVETAKQALAVEFTS"
BASE COUNT         4937 a        10911 c        10221 g         4814 t
ORIGIN      
        1 gacccccgcg gccgcggagt cgctggcgat gttgaaggag gaaccgcggg ccagcttgtc
       61 caccttggcc cggacccggc cgtacaccag cttgaagccc cgttccgggg actcggagag
      121 ggtggtgaga acgaagaccg tgttctccga gagcttgagc acccctcccg aaggcttgag
      181 ccgcagttcc aggaaggtgc ccttcccggt ctgcacctgg tctccctgct ccaggcggaa
      241 acccagaagc tcgtctgcgg gcaggggttt gttgtcccgg aggatcgtga catccgtgcc
      301 gaccgcgaat tccacctcgg cccaggccga ggccccctgc ccccacgcag cgaaggccgc
      361 agccagcagc acggccgtca gaatacattt ccgcttcata tccacagtat agcatgaacc
      421 tggcccgacc gccacgacgc ctcggattgc atcggaccgg gggccgggct atacttccct
      481 ccgatggaat ccatcctcaa cgagatccgc ctagcgtttt cggatcccca gatcaacaac
      541 ctggccgcct cggcgccggc ggaggctcgg gattccctgt actcgatgcg gttcggccag
      601 agcgaggaat acttcctgga gctcgcccgg gatatcgaga tcccccactt tcccatccat
      661 cacgacgtcc gccttcgcga accttcggag acctacctgc aggcgctgag ggccaccctg
      721 cgccagctcg tctccctcct tcccgccacc ttccgggacc tggttcatct gttcgaaccc
      781 gccgaaaccc tgaagcccgc gttcttccac ctctacaagg tccaggaatc cgtctacctc
      841 tacgtcctgc gcatcgacct cctgaaccgc cccctggaga cggacctggt ggaagcgggc
      901 acgaacgaga cgacccccgc ctaccgcacc cgacggctct atctggaaag cgagctcatc
      961 cccctgaccg ggatcgaacg tgaggaaggg acgatccgcg cgttccgggt aaggcagatg
     1021 gtctcgaaca cctggatcgg cgaatccggc aaggggtacc aggtgcgggg aatctggatg
     1081 gacgccgacc tgtccaagtt cttctccaag ctgttcctgc ccgaggggaa acgggtctat
     1141 cccttcctgc ccttcttctg caagtacaag accgtatgct ccttcgtgcc cgtgccggag
     1201 gggacctccc ggaaacgggc ccttccggtc ctgcaccggg ccctgggatt cctggaaccg
     1261 gagatggagc ggatccaggg aattcttaag ggctcgacct tctcggaatc cgacgagaga
     1321 ttcctggcgc tccgtgcccg catcccccca tcctggaaag acctgtaccg ggaggtctcg
     1381 gtccgcccgt acctgaaccc ccgggacatg aaggagtacg agcttgagta tccgcgatcc
     1441 tgaggcccga gacctatcgg gccggcgcgt caccgtcatg ggcctggggc tcaacggggg
     1501 cggtctggcc tccgcccggt tcttcgccct ccggggcgcg tccgtgaccg tcacggacat
     1561 gaaggatgaa acggccctgg cggaatccct ggccgccctg gaagggcttc ccatccgata
     1621 cgtcctgggg caccacgagc tggaggactt ttccggggcg gacctggtca tcaagaatcc
     1681 cgcggtccgg gccgattcgc ccttcctgtc cgcagcccgg gccgtggaga cggacatctc
     1741 gaccttcctc cgctactgtc cgggtcccgt cgcggccgtg acgggctcca agggcaaatc
     1801 caccaccgcg agcgccctgg cccacgccct ttccgcggcg ggcaccgctt tcctcggggg
     1861 gaacatcacg gtctcccccc tcaccttcct ggatcaggtc cgagtggaca ctccggtggt
     1921 cctggagctc tccagttggc aactgggcga cctgaaggga cgcgggctcc tccggccccg
     1981 ggtggccgtc ctcacccgga tcgtgccgga ccacctcgac cgctacccgt ccatggaggc
     2041 ctacgtcgcg gacaagcgcc tcatctacgc cgaccaggat ccttcctgct ataccgtctg
     2101 cgaccgggac gacccctacg gacgctcctt cgggaccgaa acccccggga ccgcccggtg
     2161 gtacgccgac accctggagg cggggctttc cggagcctgg ttcgaaggtc ccgtcggcct
     2221 ataccgggat ccggacggcc gcacggagga gatactaccg gagcgtctcc gggtgcccgg
     2281 tccccacgcc cgccgcaacc tcctggccgc ggccctggga gcccgcctga tgggcgcccc
     2341 ggccgggacg atcctccgtg ccctggggga gttccccggg gtggagcacc ggctggagat
     2401 gttcctggac gccgggggag ttcggtactg caacgactcc gccgccacgg tcccccaggc
     2461 cgtggaagcc gccctgcacg ccttcgacac gccgacggtg ctcatcaccg ggggaacgga
     2521 caagaacctg gacttttccc ccgtccgagc agcctaccgc agggccaagg ccatcgtgct
     2581 gctggccggg acgggttccg aaaaactccg caccctcctg gaccaggacg gaacctccta
     2641 cgaaggaccc tacgacgacc tggagaaggc cgtccgggcg gccgccgcga aaaccatccc
     2701 gggcgacacg gtcctcctgt ccccgggctg cacctccttc ggcatgttcc ggaacgagtt
     2761 cgaccgggga aggaaattca aggagtgcgc ggcccggatc gccggagtcc cggtgaccga
     2821 aaaccgcggg tccgaatgag gatcctcgcg ctggaggggg attactccgt ctatcgtttc
     2881 gatccggacc gggacattcc cgggagcctc ttggcaggct cgggcttccg gagcgttacc
     2941 cggactgacc aggagctctc ggtggtcgcc cggaccgggg ccctccgggg cgctccccgg
     3001 gaagaggcgg gctggaccct ctggatggtc caggggcccc tggacttcgg gctattgggc
     3061 gtcctcagct cgatcaccgg acccctggcc gccgcgggag tcagcgtctt cgcggtctcc
     3121 accttcgata cggactacat cctgtggaag aaggacaggg ccggggaggc cgtccgagcc
     3181 ctccgggagg cgggattcga ggtggaggga tccgcacggt ttctctgagg cggccctccg
     3241 ggattcactc gaacacgacg gagccgtacc ccacgaagct ctccgaagcc tggacgtccc
     3301 ggctcgtgta atagccgcac agcttgggat tcgtgaaacc cagggctcgg gcgaatccga
     3361 tggccgcggc agccgctccc ggggaacagg cggcgcgatc ttcgttgccc cggcgtataa
     3421 cgtcccgcga atccaaggcc agaagggcgt ccagaaaacc gcggtcgttc acgtcccgga
     3481 cccattcctg ggcctcgggt ccccggcctt tgggacagaa gctgtagctg ggcccgtagt
     3541 gggtcaggtc cgtggaggcc aggaagaagg tcttgcgacc caggttcgcg gccgcccggg
     3601 ccgcggcggc tcccaggttc tccgcggaat cgtcgttggg aacccttagc cagaggaccc
     3661 gggcctcggg gaatcgaagc cggacgaggg gaaggaggac ttccacggtg ttgtccgccc
     3721 agcggtcggc ctccaggggg gcgtaggccc ccagggcttc cgccaattcc cggtccgcgg
     3781 ggacgagtcc caggggagtc cggaaggcct cctcctcggc cgcgaggggg gcggatccgg
     3841 ggggcctatg gcctcccgcc acgatgaccg tctccgcgtc ggtccgcgcc ctgcgaaggg
     3901 tgtcccaggc caggcgcccg gaaaagtacc agcccgcgtg gggggcgact cctgcgcccg
     3961 cggtccggtc agggtccgcc tccagggtcc tctcccactc gtccaccgcg gcccggactc
     4021 cttcctcgtc gcccgggtac cagccgccct ccagggacct gttccgaatc cgcatgtccg
     4081 cccccttttc cgcttgagcg gttccgtttt ccggtttctt acagtatact cggaatccat
     4141 catggatgga tcaaaacgta cgctgtttct cgtggccagc ttggccgtgt tcctggccgc
     4201 cctgggcgtc tcctccgtcc ggctggccct ccggtactcg gagaacctgc gaaagtccca
     4261 gtccgtcttc gaaaccctta gttccgccgc gtcccggaca ctcgcgcaga ccgggcccca
     4321 ggacgaggct tggagaaccc tggtccgtac cgtgtacagg aacgacgact cggtcctcct
     4381 ggtcgtcctt catgcccccg ggaccgggat actccacatg atgcccaagt ccgctcccta
     4441 cctggacggg tccccccaga ccgcccccgt ccccccggag ctctattatc ccccccagac
     4501 cgtctcccgg ctctccgccg tcgtcggttc cgccgccggt tcgcccgtca ccctggaact
     4561 cctgtattcc ctggcgcgcc agcgggacgt ctacgaggcg gcccggctcg gactgatcct
     4621 cgccgcagcc tggaccgccc tctgtctgct gtccgccacg gccgcgaccc tccggtccgg
     4681 aagccgcacc ccggctgcgg tccccgcctc cgtttccggg ggacccgctc ccgggcccgt
     4741 cgttcccgga ccggtcctcc cggacgagga ctttcagctg ccggagatcc ccccgaatcc
     4801 ggacggtgcg gcggaccgcg gggaatccgg gccttccgcg ccgtcccggg aatccccccc
     4861 accccgggaa ccttccggac acccccgggg actttattcc ccggattccg gactcggctg
     4921 gcccgagtac ctgccggaac ggctgggagc cgaactttcc cgggccgcgt ccttcgagca
     4981 ggacctggtg ctcatggaac tctcccacga aggcatatcc cggggctccc cggcctatcg
     5041 ggtcatcatc cggatcctgc tggagttctt cagcttccgg gacctggcct tcgagcagga
     5101 cccggccggt ttggccgtca tccttccgaa catcgacgcc gaacacgccc tgcgcatggc
     5161 ggaggagttc ctgaagaagg ttacttccat tctccgggag gagggtgcgt cctcggatta
     5221 ccggaagatc ttcatagggc tgtcctcccg ctccggacgg ctcgtggact cggaacgggt
     5281 catcacggaa gcccgcgcgg ccctgacccg ggcgcgcgag gaaggggatt cccggatcgt
     5341 cgccttcaag gcggacccga acaagtaccg ggattatatc gccgcccggc cctaaagtcc
     5401 gccggcggtc ccggccgact cccggagcgg cggggcgctg ccggtccctt cccccagccg
     5461 ggccggctag gagcctgtcg gacttgtgga tatatagcgc cgataggcgc tatatatccc
     5521 gattattcgg ctcctttgga gtttgcaaag tagaaaaatc gcataaatgc gatttttcta
     5581 ttatataaag tgggcaagcc cgacaaactc ctagggccgc gagccggcgg acagctcccc
     5641 gggagtcatc agcaggtccc gaagaatccg ggcgtccagg atcaggtact gccccgagcc
     5701 ggtcagggcc gctgtatacc cgccctccgg atccggagtc ccggagatcc gaaggctgcg
     5761 gagggatccg ccgcccaatt ccaggtcaac ccgcaggcct tcccggtctt cgggcatccg
     5821 gaggctgaaa tcctccgcct ccgcccccat ccaggaccgg acgaggcgtc cggccgcggc
     5881 ggtatccagg tccggaatct gggggctcgt ccaggctccg gacaggccgc gctccaggac
     5941 ataatccgcc cggcgaacgg tcccgtccgg gaatcttacg gagccggata cccggaggcg
     6001 ctggacgtcc tcccggggaa ccggctcgcc aaaaacccga aggtccagcc aggccatccg
     6061 tcccgccgga agccgggagg ctgtcgcggc cggggccgcg taagcccgag tctccccgct
     6121 cttccgcagg aagaccctcg acccgtccgg ggcgtacctt cccacgcgga aatccgccgc
     6181 tgacgctccg tcccgaagga gaagccgaat ccgccgggag gatccctcgt ccagcccgag
     6241 ctcgtcccag gaggcttccc tccgggacac ttcgaccagg gaacgaacgg aatgtagggt
     6301 ctccaggtag gcctcgatcc tctcggtccg ggccggaagg ggaaggccgt cctcctccag
     6361 ggtccaggcg gcgccgaacc gccggaagac gagggaaccg tcgggcccgg atacctccac
     6421 ggcatggatc tccgcgggat ccgggaccag gagccgaatc cgggaagccc gggcgttccg
     6481 ggcccccggc gaaaacgcga cacccgcggc gaccaggagg gccaggaacc cggcgaccgc
     6541 ggacaggacc gccgccttcc tccggaaagc cgtcatgccg ctctcccctc ccgctcccgc
     6601 cgggtccggc gggaagccct caggaaggcc gcgagtccca ccagggcggg gaccacgagg
     6661 atgttgaccg cgtaggccgc tccgacggcc cgccttcgga cctcttcctc ctcgggcagg
     6721 cggagccgcc gggggccccg ggccgcggac ggcaggggaa ggtcgtcccc cgcggaaagc
     6781 cagtccgccg ccgaaaccgc gaacaggata ttggactcgc tgccgctcat ccccgcgagg
     6841 tccgtgagga aatccgagga acccgccacc accaggcggc ccgggcgccc tgcgttcggg
     6901 gaagtcgggg cgggtgccgg agcgagccgc ccgtccgggc cgggccgccc ggccaagccg
     6961 ggcacggcat ccccgtacag ccgtgggaag gtgccggaca gggccgcggc cagtacatac
     7021 tgtccccggg tggcgtcctc ctcctcccga tagagggatt cctccccggg ggccgcggcg
     7081 aaggctcggg tctgtttcca ggcacgggtc gaggacttca ccagggcttc gctggaaagg
     7141 cccggacggt ccgagagctc cagggggctg ggccagtata ggtcgaggcc ggagaacccg
     7201 gcggccgcgg gatgcgagac ggtcacattc tcccgctcca cggccaccca gtgggggtac
     7261 cgcaggtatc ggtaggtggt ttccccgccg gagccctgga cctggaaggg cacggagagg
     7321 ctggaagcgt cgaggaccag ctcgcgccgg acccggacgc cgtaggccgc caggagatcc
     7381 aggacgggat cggcccccag ggcccgggct tccaggttcc ggtcgggatc cacgcggacc
     7441 ccccggacgg cggcgaggac ccggccgcct ccggcgaggt agcggtcgat ggagtacgcg
     7501 tcccgctcgt ccagggcggc gtttcccagc acgaggagga cggagacgtc cggttccacc
     7561 tccgagccgg ggggcgaaag gcggacctcg tagcccgccc gggcgagggc cgcccgcagg
     7621 agacggaagt cctcctcggg atcccggttc ccgtccccct cccggagggc ggccacggga
     7681 acggtcccgc ccacgagtcc caggacggcc ttgacgacct cgtactccag gcgttcgggc
     7741 cccaggacgg agggcaggac gcggatgtcg tccaggtatt ccgccacgac tcccgcgtag
     7801 accgtcgcga tccgggcctc ccccccgccc tccacctgga gctgccgggg aaggattccc
     7861 agggactcca gcctcccggg atcctcggag gcgtcccgga cggatacccg gatacggcct
     7921 tggctcacgg attccatctc cgaaagaaga tcctgggcca tccggatccc cgggtgcagg
     7981 tccgccaggg ccgcggaccg gtagtaggtg aggttcacgg tgtccgggat ccgggcggcc
     8041 agggaacggg acgccggcga gagggtgaag gccctatcct cggtcaggtc gatccggatc
     8101 cggtacaggc tccccgccgc ggccccgagg gcgagcacca ggagagtaag gcccttcagg
     8161 atcccttcct cccgcagcct ccgggccgcg gtatcggagg tcttcatcgc caggcctccc
     8221 ggaccagggc cttggccgac aggtagagcg ccccggaggt caggatcccg aagtagacga
     8281 ggtccttact gtccaggacc ccccggacga aggactcgaa gtgggaggcc ggggagatcc
     8341 agacgaggac ccgggcaagg aagcccggca gggtcaggaa ggcgggcacc cggtggaccg
     8401 cggacagggc taaaagcagg gcagaggtgg ccaggtaggc ccctgcggga ctccgggacc
     8461 gggaggaaac gtactgcccc agggcggcgc aggcggacgc cagggccagg gctcccaggt
     8521 attccgtgac caggacaccc gcatcgaaat ccccgtaccg ggaaagcgtg aggggcaccg
     8581 agaggctcaa gcccaggacg gctccgacca ccgccatcac cgccaggtgc ttgccgacca
     8641 ctaggtcccc ggggcggagc ggcagggtga ccaggatctc gtacgtcccc gtcctgcgct
     8701 cctcggccca ggaccgcagg gtcagggcgg gaaggagtac cgcgaagagc gccggcatga
     8761 cctcgaagta gggccggagg gaagcctccc cgcggacgaa gaacccccgg accaggaaaa
     8821 gccaggccgc ggacacggcc aggaagaagg ccgcgaagat gtaggccggg gcggtgttcc
     8881 ggaaggagcg aagctcccgg cggaagacgg ccaggacggg gctcattccc cctcctcccc
     8941 ggtcagccgg gcgaaggcct cttccatgga gagccgctcc tttttcaact caaggagctt
     9001 gaacccctcc gaggcggccc agtccgaaac cgattccgcc gggtccgcgg ccccggccgg
     9061 gaaaccgagc cgcacccgcc aggcgccgac cccctccgga ctcggcggcc cctcgaggcc
     9121 cgcccctgcg aggcgggcca gggcggcctc cacctccggg gcgccgggag ccttgataac
     9181 cgcctccagg cgatcccggc tcgccaagcc ctcggagatc tcccggggac tccccttccc
     9241 ggccagggcc ccccggaaca ggaccaggat ctccgagcag agggcctccg cctcccccag
     9301 gatatgggtg gatagcagga cggtccggtc ccggccgagg tcccggatga gctggcgggt
     9361 ctgtcggatc tggttggggt cgagtcccgc ggtcggttcg tccaggacga ggacgggcgg
     9421 atcccccagc agtgccaggg cgagccccac gcgctgtctc cagcccttgg acaggcgttc
     9481 cgccgggatg gcccgggcct ccgcggcccc ggcgcgctcc gcggcctccc ggatcgcccg
     9541 ggccttggcc ggcccgcgca gtccccgggc ttcggccgcg aaagccaggt gctcctccgc
     9601 cgtcaattcc ggatagaggg gggcgttttc cggaaggtat ccgacacgcc gcttgagttc
     9661 ccgggaatcc tcggcggcgt cgatccccgc gacccgggcc gtgcccgagg tgggaccgtg
     9721 gtagccggcg aggatgcgga ggatcgtggt cttcccggcc ccgttgggcc ccaggatgcc
     9781 gaccaccccg cccggtcccg cgtcgaagga gacgccccgg accgcctcga actcgccgta
     9841 gcttttccgc aggtcccgaa cctcgatcat ccggcgcccc tagtccaggt actcgatggg
     9901 gatgcacatc cggtcccggg acaggacggt atccgggaag atctcccggg cttcttccag
     9961 gagccgcttg aggttctgtt cggtgtagcg ggggctgtag tggatcaggg cgagcttctt
    10021 gacccccccc gcgtccaggg cgatccgggc cgcctgctcg gcggtcatgt gccgcttttc
    10081 ctgggcgctg gccaggagtt cccgctcgaa catcccctcg cagaccagga ggtccgagcc
    10141 ggacacgttg ggggctatct ccgggaaata cagggtatcc gtgacatagc cgaatttccg
    10201 gcccgaccgg ggcgctccca tcacctcttc cggccggact acccttccgt ccggaaggtc
    10261 cacggggctg cccccctgga gcacggacca cagggggccc cggggcacgc cggaggcttc
    10321 cgccttttcc ggaaagaagg cccccggccg cggatcctcc tccaggacgt agcctacgca
    10381 gggcttggtg tgccggaggg gaaaggcccg gatccggaag ccctccccct cgtggcagag
    10441 gcagggggct tcgatctccc ggaccacgat ctcgtagttg atgtacatgt ccagaaccct
    10501 gcggctggac tccacgtatt ccgcgatccg tggagggccg tagatgtaca aaggttcgtt
    10561 ccggtccacc tggctggaga gcatgaggat gcccggcagg cccgtgacat ggtccgcgtg
    10621 ggtatgggat atgaagatcg cgttgatctt cttccaccgg aggttcaggc ggcgcaggga
    10681 gacctgggtt ccctcccccg cgtcgaagag gaagaggtcg ccctcccggc gcaacaggac
    10741 gctggtcaga tgccgatggg gcaggggcat catgcccccg gtacccagga tgaaggcttc
    10801 cagattcatg gagggcttac tataccgaca agtccgtccc taggcaagga cggccgagct
    10861 tgtgcgggcg acccttttca tgccacaatc ggcagatatg gttgaatttc agagtctcgg
    10921 aatccgggaa gacctggccc gggccctagc gtccttcggc ttcgagggtc ccacggagat
    10981 ccagcgaagg accgtgcccg ccctcctgga ccggtcggac gccttcgtca gctcctccac
    11041 cggcacgggc aagaccttcg gatacctggt ccccctgctg cagctggccc ccggggagac
    11101 ccggaagccc ttcgccctgg tcgtggcccc gacccacgag ctggcggttc aggtggagaa
    11161 ggagctggac cgcctggccg ccgccgcggg gatccgggcg gacacggtcc tcctcatcgg
    11221 gtccgcccag gctcgcaggc aggcggagcg gctccggaaa ggcggttcct tcgtcatcgg
    11281 cacccccggc cgcgtacggg acctccttgc ggcccgggaa ctcgacctct cggagctctc
    11341 cttcctggtc ctggacgagg cggaccgcct cttcgagaag gaggccctgg cggagaccgc
    11401 ggacatcctg gatgccctgc ccgaggcctg ctgccgcgtc ctggtgtccg ccaccctccc
    11461 ggaccgggtg cgggacaagg ccgccccgtg gttccgcgct cccgaggccc tggtcctgga
    11521 caactcggag gtcctacggg agaacatcga ccactgggtt ttccactcct cgtcccgaag
    11581 gaagatcgac ttcctccgac gcttcgagtc cgcggtgcgt ccgaagcgct gtctggtctt
    11641 cgcctcctcc aacgcccaga tcttcaacat cctgcgaaag ctggagtacc tggaattccc
    11701 gaccgcggag ctccgggccc agggggacaa ggagtggagg aagcaggccc tggacgactt
    11761 ccgggagggc cgcgtccgat acctggtgac cagcgacctg ggagcccggg gcatggacgt
    11821 gaaaggcatc gactacgtgc tctgcatgga cctcccggag gagcccacgg tctacatgca
    11881 ccgcgcgggc aggaccgggc gcgccgggga gcggggggtc tccgtcctgg tggccgacat
    11941 cgtggagctc aagcgggctt ccaaggtggc ggttcgctac ggtttttcct tccagtgccg
    12001 catcctggag ggcggggagg tccacgagat cgatcccgag aacttcttcg ccctggcgga
    12061 ggaggaggag gccgaccgcg ccgtcaagcc gcccctggag gccaagaggt tttccgacat
    12121 ggaaggccgc cgcccccggg ggcgcctgtc tccctcggga ggcgtaccgg cgggggcgcg
    12181 gtcccggaaa ccccgcgcgg gctccccggc gctccggcag gaaacccgaa ggccggacgg
    12241 accgtccggc gccgcggaaa cgggcccggg ccgggggcac cggccttccc agggaagacg
    12301 cggcgccccc ggcgcgtccg gtccagccgg acccggcaaa tcccgtcccg acggacggag
    12361 ggacggccgc cggaaaagat ccggctaggg tcgagcccta cccgttccgg agccgttccg
    12421 cgcgctggac ccgaagtcgt tccagtcccg acagcagcct ctcccccagg accggcaagt
    12481 cctcgccgag ttccgtcagg atttcctcgg ccctctcgta ctcccgcacg taccggggat
    12541 gggccagctt ccgcagggac cccagggctt ccgcggcggc ccgacgggcg tccgcgaggt
    12601 cccgggaagg ggcgagcggg ggtccgcggc ctccgcagtc ccgggcgacg gccgccagca
    12661 cctccaccgt ccgggcgtgg tccgtcactt cgcaggcttc cagacgcccc ccgaattccg
    12721 tcagatcctc gaccccgacg gccccgaggt ccggaagggg gtcctcgaag cggtggaacc
    12781 gccacatcct cagattgccc aggggggtgg cgatgcccgg ctcccccggg gagggctcga
    12841 aggtttccgg aattttccgg gggtcgaagc gggaggagaa caggcgccgg agcttcagct
    12901 ccgcccggag acgggcaagc ccggagacgg atccccgggc accgctccgg gcccggcata
    12961 cgtacaccag ttcatggttc cgcaccctgc gggtaccgct ctggcgcccg ccccggtagc
    13021 gcacgtagtc caggacctcc ggtcggacct cccagcgccc ggacagcagc tcatagagtt
    13081 cctccgggga gaccaggccc tcggtgttgt agctcaggac cagggttcgg gcatccaggg
    13141 cgtcgatcag cccggccagg gccgcccggg ccgtggaacg tccgcagaag ggggagcggg
    13201 tctccttcca ggccggtggt atgcccgcct ttcggaggag ccgtccgtcc ggcccggttt
    13261 ccaggggaac gggatcgcga tcccagcgca cgagggtgtt caggatatgg taattcgagc
    13321 cgtactggtg ctgattgtag ggaggatcca ggtacgccag gtccgcggtc cagcgacgga
    13381 cgaagacggc cgcgtcctcg cggaaaacct ccgccggctc cgcctcgggc aacagggggt
    13441 gctccagctc catggccccc aggatacggg gcagtgcgtc ccggccgtgg cctccgaagc
    13501 ccttgtggaa ggccttgaat accccggagg tgttcgcgtg caccgccgcc tccaggacca
    13561 gcagggctaa aaggaggtcc cgccggcggg aggccgggga gtcggggaaa ccggactcgc
    13621 ccgagggata cgcgcgttcg atgtgttccc ggacccggtc caggaagacc gcgttctccc
    13681 gggtgtagaa gagtcgttcc accctccaat ccgcccggga ggtgtcccgg ggggcgtacc
    13741 accgggccat gtagggctcc ggctccggat cgggggacag attgtcccgg gcggggtgga
    13801 aggagttcag gcggtccagc caggctttca acccaccctc ctccgcccag aggtccggga
    13861 gatccgaggc ccgaaggacc agccaggcgc gattcagggc gtaggcataa ggttcccagt
    13921 cgttcgcctt gacccgcatc cccagggtcc gggccagacg ggacaccgag ccggatcccg
    13981 cgaagggatc caggaagacg gggtggttcc ggccctcggt cagggaacgg aacagggagc
    14041 ccagatgccg ggtcagggac ctcttgttcc ccagatacgg aatgagctgt cccgtgacat
    14101 actcccgctc ccgcggaacc gcctcacggt tcataccggg ccatgctcgt ggcgctctcg
    14161 aagacctcca cggcctgcag gggcgttccc gggagtctcg cggccaggga atcgaagatg
    14221 taccgggcta tccgctcggc gctcggcgag tccaggaagg ccgggttctc gttcaggagc
    14281 cggtggtcca gcccctcgca gacctcccgg agggctttct tgaacacccc gaaatccaag
    14341 agcatcccgg attcgtccag gtcgcttccc cgggcaaagg cccggacccg gtagttgtgg
    14401 ccgtggaggt tctcgcactt gccccgatag cccgaaagga aatgggcggc cgaaaagtcc
    14461 gcctcgactc tgactgtgta catggcgtct ccggacggca ttgtacaacg agggccggaa
    14521 aaagtcccgc gccgcgtgta ggcggcggct acagtcccag gaagctgtac acggcgctcc
    14581 agaaagtgtc ggcatccatg cgcccttcga ggaaagcctt cacgaaggcc ttgagctccg
    14641 ccgggaaggc ggtccagtac tcctcctgcc aacggggcat gcgctcccac agggcctgct
    14701 gggcgctggg aggcatcaag cccagggaat aatccgtgaa gtccttgacc atcttcgcca
    14761 gggcgtccgg aggcagggga gagacctcgg cgggtccgcg tgcccccgga tccggtacgt
    14821 aggaggcgag cagttcttcg agctgttcct ccgtgacctc cggggcgttt ttccggatga
    14881 tgtccgccac gaagtcccgg atcatcgacc gaatcccgtc caggctggcc cccatggagc
    14941 cctccaccgc ctgagcggtg ttccgggccg cccgttccgg gttgacccct ccgatgctcg
    15001 cgaagaggga caggtccctg cgcctgcgct cgcaggcctt gacgatggcc tcgaactcgt
    15061 cccggtcggc cttgttgagg atgtagtcca ggacggatcg gagattttcc cgggacatct
    15121 tgagtccact gtacccccgg cggattccga gcgcaagcct tggcgccgga ttccatcgcc
    15181 cccgatcttc cctgtacacc gtccaccgtg cgctgtttac cgtccaccgc gcctcagggt
    15241 actcttccga ccggagaacc acggcgtgtc ggagaacgcg gaatcggaag ccgggcggca
    15301 ccttctcgag cccctgggac aggtcggaag cctcatggga gagatcccgg agccggagat
    15361 cgcctttcct ggacggagcg ggccgaggga ttcccgaggc cgcttacgcc tcgacgtctg
    15421 cctggccgtc tccctcgcac tgcacgccct ggtcctggca ctttttcccg tcccgggcac
    15481 cggagggcgg ggcgcggcgg catccacggt acgggtgaac ctgatcctcg gtacgggcgc
    15541 cgccgcgcgg gaagcggcgg ggtccgtacc atccggggaa ggcgccgccc cggcggctcc
    15601 ttccccgggc gccccggacg gctccgcttc cgacgctccc gaggattccc ttccccggcc
    15661 cggggaggcg gaacctgccg gcggtgagga gccgggcgcc ccggactcgg cggtatcgcc
    15721 gtccgccccc ggcgcccctg cggcgggttc tgccgctgga tccggcgggg ccggctatcc
    15781 ttccccctcc gaccgggcgg ccgccctgct cctggcccgg gtggaggcag ccctggtata
    15841 ccccgaagca gcccgtcgcc ggggcaccga gggggtcgtg ggactgcgga tcctcctgga
    15901 cggttcgggc gccctcgcgg acttgaagat cacgcgctcc tcgggcagtt cgcttttgga
    15961 caaggccgcc cgggagacgg tcgcagcctg cctcccagtg ccgaatccct cggggatacc
    16021 cctggccttc gagcttgcgg ttcggttcac cctgaaaaaa gcggcggccc cgtaggaacc
    16081 gccgccctgt tgtcccgaag ctagagaaac gatacccgtt caaccccctc agtccaggag
    16141 gatgcgcagc ccggccttga gggtgatgcc gggcatgggg tagccgtcca tctcggcgta
    16201 ggcccggttc aggaggtttt caccgtccag gtagacgtcg gcgttctcgg ccacccgaag
    16261 ttccacccgg gcacccagga gaaggacggg atccagggtg atcgtcgggg actggtagcg
    16321 ttcgctccgc caggagcccc tcagcgagac cttcccgaag ccgtcggaat agacgggttc
    16381 gagcttcacg gtatgtacgg ggtaattggc gatccggacg tcgttcgcca aggtccttcc
    16441 gtccgccaaa tcgtaggtta gtaacaggcc gtacgaggcg ctgactgata ccggcccggc
    16501 gtgtacggca accgccgcgt cagcgccgat gaaggccgcg gcggccaggt tttccggacg
    16561 ccagacgaaa tcgccgtccg gatcccccca tcggatgaga tcgtccgagt agcgggcgaa
    16621 gaggttcacc gctgtctcga ttcggctccc cagggtttcc aatccgatct ccccgtacca
    16681 ggaggtttcc gtcaaaagat cgggattgcc ctccgtccag ggatccgcag gccagtacag
    16741 gtcgttgaag gccggggccc ggtaggaccg tccggccgtg gccttcaggg tcacgacctc
    16801 ggataccgcg gccgaggcgc ccagggagta cgtgagcccc gccgggtagt cgtcgtacca
    16861 gtcgagacgg acggctgggg agaccttgac cccacgacct acggcaagga cgggtgccgc
    16921 gtacgccccc gcgtggtacc gcctgggatt gccgaccttg gtgctctccg ccccgtcgta
    16981 ccggaactcc aggcccgcgg ggagttccac ggcatccgag gccagatacc ggaaccggaa
    17041 atccgcgccc gaggagaagg tgtcgtgtcg gtcatccgct ggctcgtccg gatcctcgta
    17101 ctccatgcgg gaccaggctc cgaaggccga gacatccagg gacagccgtc ctctcatcag
    17161 ggcgtcgctg gaccaggcgg cggttccccg gaagtcccgg ttccgctgtt cggcgttcgg
    17221 cgtcagccat gtcaggatgc ccggcacccc gaagtcctcg tagcgccccg aaagggccgc
    17281 cgagaggatg ccgcccagga ccggaacctc gagccccagg gatccggagc cccccaggaa
    17341 ccccgcgttt ccccgttcca gaacggcgcc tccggcctgg gcggccggga aggagtctcc
    17401 cgcccgctcg aaggagccgg aggcggacaa gcccgccgcc ccgatccggg tgtccaggga
    17461 ggctcccagg cgctgggccg cggctagata ctcgagatcc gcgcccaggg ccgcgggata
    17521 gaccttgttc tccgcggtca cggtcagccg cgggtcccct ccccgcttgg tggtgatcac
    17581 gatgaccccg cccacggcgt cggcgccgta cagggaggac gccccgccct tgagaacttc
    17641 gatccgctcg atggactcgg cgggaatctg ccccaggtcc acgttccctt gccgggcgtc
    17701 gttgagccgc gccccgacca ggaccaccag gacctggccg ctcgaggccc cccgcaagga
    17761 gggcgcccgg acgctcccgg ggttcccgta gtccagggaa tggaagcccg ggaccgaggc
    17821 cagggcttcc gcggcgttct tggccccgga ggcctcgatc tgctccgccg tcaccacgac
    17881 gacggagccg cccggggcca gggccgcggg gctccagctt tcggccgtga cggagacctc
    17941 tccgaggtcc cgtacctgcg cgccgggtcc caaaaccgcg agggccgcga cgaggatgaa
    18001 acatgccgcc ttgcgcatgt tccgctcctt tccgctcctt cctgagggag cggcggaccg
    18061 gaaggcgtgg accgatccta gggcaagcag ggagacgacg ggtctgccga gactgcgcgc
    18121 cgcccggggc ggcttccagg gagggggtgc acgacgactc ctcgatccgc gaaggttccc
    18181 cgcccggaca gcccgagggc gcccggatcg cgggggagcg gagggccggt atcctggctt
    18241 ccggatcgtc gatgtacgcc gacgccttct cgggcttcat cgcccaatgg ccgcgttcgg
    18301 gtccgcattc ctgtggaccc gaacccatgt cggcattctc cccggttaca gtggcgggac
    18361 cgccgcggcc ttgcaccgcg ttccacggat ccctcgctcg tcgcgggaca ggatgcccgg
    18421 aaatctccgg gccgtcaagg ccggccggga gaattgcgtc cggtcaggcg caggggcggg
    18481 cgccggccgt ctactcgaag aacctccgcg acaggtcccg ggagtagcgg atcaccctct
    18541 ccacggtgga gaaggacgcg atctccccgc aggcgtaggt ccggatcctg ccctgcaggg
    18601 cctcgaaccg atcgtaccaa ccggcccggt agtcctcgca ggttacgtgg gggtagtact
    18661 tccagcggac ccgggagacg gtttccccgg gctcccctcc cagggtccgg atgtcctcgt
    18721 ccaggccggc ctcgatctca gcgtcggact gttttccgtc ccccaggacg tagaatgtat
    18781 ggatcccgct gccgggaacc cggtcgtccc agatcatggc ccggccgatt ccggagcggt
    18841 cgaaccgcgc cggggcgaag cccgcgggga cccggaagcc tcgggcccgt ttgaggtaga
    18901 cccgatagtc cttgtgacga attttcgtgt aaagctcccg ttcctccgtc gtagcgtcca
    18961 ggaatcccag ggaatcgtcc agggggcagg ccaggaccag acggtcgaac tcgaaccgga
    19021 agggctcgcc cgagctccgt tcccggcccg agacggcgac ggtcccgccc cgacggatct
    19081 cccggacgtc cagggagtac cggacgtcca gactctcggc caggcgctcc cagagggtct
    19141 ggacgccctc cctccacttg aagaacttga actggagggc cagggcgcgg acgatcccgg
    19201 gttcgaagaa cttgagcacg taggccgccg gggactcgtc gtagtagccg tatccgaagg
    19261 tcgtgtaggg aatggagatc gccttggcca ggacgcccat gcggtgacgg tccacccagg
    19321 cctggaagga atccgccaac tccccgggag tgcccaggtg gttcggcccg aaggccgccc
    19381 gatagcgggg cgcgaggata tggtagcgga ggatctcgag gatgccctcg gggagttccc
    19441 cgatcccgat ccaacccggg aagagcttct cgcagggcca gaacccgcgc ccgacgaagg
    19501 cgtcctcggg ggtccggtcc ggggcatagg agcgccaggg atccaggccg acccggcgca
    19561 tcaggtccag ggtatcccgg tagtccgtgg tgcccatcac cgcgcccagt tcccagttgc
    19621 cgccctcggc gcggaaggtc cggcacttgc cgcccggctc ggattcccgc tccaggaccg
    19681 tcacggaccg gaaacccgag tccgctaggc gcagtgccag ggaaagcccc gcggccccgg
    19741 cgccgagtat cccgattctc tcgtttctat cgaagcccat ggcacgcctc cgttccccta
    19801 gcggcccttg tccgcgcggg aatcccggcc tatagtcgaa ccgtgcacga attgccgatt
    19861 ataacacgag tcctcgagtt gtcgctcgcg gccgcgcccg agggctcgga gatctgccgc
    19921 atcaaactcc gggtcggcag cctctgcgac gccgaacccc tctggctcca gcggtatttc
    19981 cgcatcgcgg cccggggcac ccccgcccgg gacgccgagc tggcggtccg ccgggtgccc
    20041 gcgggctcga cggaggacgc ggtccgcgac ccggactccg ccttcgggta cgtcctggaa
    20101 tccatcgaga ttcgggacgc ccccgtagga gctgtgccgt gagcggaacc tcccgtatca
    20161 tcgacgtgcg ggcctcgatc ctggaggaca acggaaaaaa ttcggaggct ctgcgccgat
    20221 ccctttcggg ccgaggggtc ttcctggtca acctgatggg ctccccgggg tccggcaaga
    20281 ccagcctgct ggccgccctg ctccccctgc tctccccgcc cctccgaccc gccgtccttg
    20341 aggcggacct ggattcccgg gtggacgcgg acgccctgga agcccggggt tggaaggctg
    20401 tccagctccg cacaggggga ttctgtcacc tggacgcctt catggcacgc aggggcctcg
    20461 cggagttggg cgtcctgccg ccggaggacg gcatccccgg tgttccgaat gatgccggtc
    20521 cggactcgcc cttcgacatc gtcttcctcg agaacgtggg caacctgatc tgcaccgccc
    20581 aggtggatac gggggcgcac ctgaacctgg ccctcctgtc cgttcccgag ggggacgaca
    20641 agcccttgaa ataccccgtg atgttcgcca aggcggacgc ggtcgtggtg accaagacgg
    20701 actacctgga ccgcgaggac ttcgacgtgg aggcggttcg ccggcgtgtg gaagggatac
    20761 gaccggattc ccggctcttc accgtttccg cccgtaccgg aatgggcatg ccggagctcg
    20821 cgcgcttcct gcaggaaaag atccggacct aggaccgccc gcggcggccg tggggatggg
    20881 tcttcagacc caggataccc gcgattccca gcctcagctt ccggagcgtc ccgggacggg
    20941 acgcggccag acggtccagg agttcctcgg tgtttttcgg aggcacccgc gcgggcgtga
    21001 ggggctccag ccgtagggct tcgaacttgc aggccgccgt gcaaaccccg cagccgatgc
    21061 accggctttc caagattcgg gcgccgctcc gtcctccggt tttcgcggcg gggccgtcca
    21121 ggacgatcgc ctccatgggg cagatccgcg cgcaggcccc gcaggacacg cagttccccg
    21181 gcagcacgac ggcgcggtgg ttcgtggcca cgaagtccgc ggggcgcttc atcgccttca
    21241 cgacggaaag gagtccgcag cagtccccgc agcaggcgca gaggaactgg ggctccctct
    21301 cgttggaagg cctcatcacc agtccttccc tctggttgag gtccgccaat tccagtgcct
    21361 cggcctcccc gaccgcacgg ccccacccct cccggatcac cgtatccgcg aaatcccgga
    21421 ataccatgca cagctcccgc cggtccgtgg atttgcaggg cttcccggcc aggtccgtcg
    21481 cacgacggca gatgcagtcc aggaccgcga tccggcctcc ggcgttccgg atcaggaatc
    21541 ggcactcgtc gtactccgcg atcctgtgct cgggccgtat ggccgcgtct atcgggatca
    21601 cccgggtctg gggctccccg gtggtcagaa gctccagccc gaatccctgg tgcaggaaat
    21661 ccaccgtatc ccggaccagg tcccgggaga gtcgggctat ctgcatctcg tacatgccca
    21721 ccacgaaggg caggagggcg taggtaccgt ccgtcgcctt ccggaggatg ctgcccttcg
    21781 aagccagggc gccgagaagc gaggcgaggc cggagccgtc cggggcgccg gagggctccg
    21841 cttcggcccc ggcctcccgg gcaagctcgc gcggatcgag accccgggct ttcaacgccg
    21901 tctcgacctc cgcggggctc cggaatttcc aggaaagacc cagggcgacc cgggcctccg
    21961 cgggagtgaa gagcctgcgg aggatgcggc cccccgcccg gctccggggg aaaccgacgg
    22021 ggagggtgtc gaggtgccgt cggagctccg cgtaggcttt ccgggtatcc atgcccggag
    22081 catgacggat ggccgccgcc gcgtcaaccg gcgcaggctg gccaaatcaa cggatgtttt
    22141 atccccgaat cggggatata ctatcgagtg tacgggcgtg cctcggcggc gggtaccgcc
    22201 gccgaggcac gccgaacctc gaacaaggag gcaaccttga agaaagcgat catcctggcg
    22261 gccctggccg ccatgctggc cgtcccggcc ttcgcgcagt ggcgcgtgga tatcggagcg
    22321 gacatgccct tctacgcggg catcacggat tcgatcgtcg gcgacgccac gggggggaac
    22381 atcccgtatt tcatcccctt cccgaacgcc gcgctcagct accagttcga tcttggactc
    22441 atatccctgg gagtcggggc gaaggtgtat tccttcatac tcctgaacat cctctccccc
    22501 atggcgtacg tggagatgga tttcgatccc ctcgtggtga accttagcgc ctggggaggg
    22561 ggattcctcg tgttcgggct cgtgaacgag ttcctcacgg agaatttcat cgtccccgac
    22621 ctctccgtga tgttcaagct cgggaaacgt cagaccctcc ggctcggggg aggggtcatg
    22681 accgcgctga gcctggaggg aagccagggc gtgttcccct tcatcgccta cggcactatc
    22741 aagttcagtt tccccctctg agtccgccgt ggtgaagcct gcccgggacc cggttccggg
    22801 caggccgcct tgaacaaggc cgcgagcggc gctaccatag gccgcatgac gagccgtctc
    22861 tcggacctcc tccccttcct gcccgcccat gaactcgcgg gccccgcgga tgtaccggta
    22921 accggcatgg cctacgattc ccgggacgtg agacccggat acctcttctt cgccctgccg
    22981 ggcctccacg cggacggcca cgatttcatc gacagggccc tggacgccgg ggcttccgcg
    23041 atcgtccact cccgggatcc cgggcctttc cggcaaggca tcgcctacgt gcgggtgccc
    23101 gactcccgct tcgccctgtc ccccgcggcg gacgcattct acgggcatcc ctcccggagc
    23161 ctcggggtcg taggagtgac gggaaccgag ggcaagagca ccacggtcta cctgatctgg
    23221 cagctcctga acctttcagg agcccgggcg ggcttcttct ccacggtcat gtccgacacg
    23281 ggatccgggg aactgccgaa cccggagcac cagactaccc cggaggccaa cgccgtccac
    23341 cggatgctgg cggacatgcg ggatgcggga tacgagtacg cggtggtgga ggcttcctcc
    23401 cacggcctgt cgccccgcac caatcgcctg ggggacgtgg ccttcgacgc gggggtcatg
    23461 accaacgtga cccacgagca cctggagttc cacgggaccg tggagcaata ccgccacgac
    23521 aaggccaacc ttttccgcgc cctggaccgg gtcggacacc ggaaagtgat cgccggacgc
    23581 tccgtggagg tcccctcctt cggggtggtg aacgccgacg accccgtgtc ggacctgttc
    23641 cgccgggcct gcggaaagcc cgtatactcc ttcggcgccg cggacgcgcc ggcggacgcc
    23701 ttcgcccgag ccgtggcctc cgacgccgga ggctcctcct tcgagtatgt cgaggaaggc
    23761 tcgtcctatc cggcccgtat cgaattgccc ggggagttca acgtacgcaa cgccctggcg
    23821 gccctgctga ccgtctcccg cctgaggggg atcccggcct cggaactcct gccgtttctc
    23881 ccgaagctga ggcccgtacg cggccggatg accgtcgtgg accggggcca acccttcgag
    23941 gtgatcgtgg actacgccca caccccgtcc tcgttccagg cgatcctgcc tcccctgcgg
    24001 agccgggtcc ggggccgggt gatctgcgtc ttcggctccg ggggggaacg ggacacggaa
    24061 aagcgtcccc tccaaggccg catagcggcg gaatactgcg atgtcgtgat cctctcggac
    24121 gaggaccccc ggggggagga tcccatggcc ctcctggagg acatcgccgc gggctgcccc
    24181 ggcctagtcc ggggggaacg gctcttcctc atcccggatc ggcgggccgc catccgcagg
    24241 gccttctccc tggccgggcc cggagacctc gtccttctcc tgggcaaggg acatgagaac
    24301 tccatcatct acgcccgggg gaccatcccc tgggacgaga tagccgaggc cgaagccgcc
    24361 ctggccgagc ttgactacga aaggtcctcc ccatgaacac cgtgatcctg tacggaggcc
    24421 gctccggaga gcacgaggtc tccctggcct ccgccgccag cgtcctccgc caccttcccc
    24481 cgtcccacga accgaccctg atcgggatcg accgggacgg cgtctggtac cttcagccgc
    24541 cccgggccgt cgaaaaggcc cggggcggat ccggccccct ggagatcctg aaggacgagt
    24601 cccgggtcgc ggcggccgtg ccgggccggg gcttggccct cctctccggc gaccccatcc
    24661 ccgcggacgt ggtcctgccg gtcctgcacg ggaccttcgg ggaggacggc accgtccagg
    24721 gtctactgga gaccgcgggt ctggcctacg cgggagcgga cgtcctggga agcgccgtgg
    24781 ccatggacaa ggaggtctcc aaggccctct gggcccgggc cggcctaccc gtcccgccct
    24841 tccgcacggt ccgtgccgcc gatctcgagc ccgcccggtt cgaggtcctg cgctcggacc
    24901 tggaggcttc cttcgggtac ccgatgttcg ccaagcccgc ctgcgccggc tccagcgtgg
    24961 gcacctccaa gatcgataac tccgccggac tcctgccggc tctccggtcc gccctggagt
    25021 ggggctccaa ggccttggtg gaacccttcc tgcaagcccg ggagctcgaa tgctcggtgg
    25081 tgggcaacgg ggagccgacg gcctatcccc ccggggagat cgtgagctcc cacgagttct
    25141 acgactacga ggccaagtac gtggaccccg acggtgcccg ccttctcatc ccggccccgg
    25201 tgccggcgga gatctcccgg ctcgtcatgg acatcgcggt ccgagcctac aagctggccg
    25261 ccctgtccgg gatggcccgg atcgattttt tcctggacag gaaggacgga tcggtttacc
    25321 tgaacgaggc gaacaccatc ccgggcttca cccacatctc catgttcccc aagatgtgcg
    25381 aggccggggg actgccgtac ccggagctcc tggatctgtt ggcccggctc gggatcgagc
    25441 gcaagcggga gagggacggg atccggtaca cccgatagcc ccggattccc ggctttcgga
    25501 ctcgtatccc cggggccggc gggacccgcc gcccgccggg agcgggtccc ggggaacccg
    25561 ggcgcataaa gatgcacggt ctcttctata aatatctata ttataagaaa ttagcaaaag
    25621 gcctcttggc gggatcccgc tcgggatgct atactggcga cctggttcca caggaaccga
    25681 ggaggaatcc atgaagacgc aggaatacat cgatctggac gagaaatacg gggcccacaa
    25741 ctaccatccc ctccccgtcg tcctctccaa ggccaagggc gtgaaggtga tcgacccgga
    25801 gggcaaggag tacttcgatt tcctgtccgc ctattccgcg gtcaaccagg ggcatctgca
    25861 ccccaagatc gtcaaggccg ttcgcgacca gctcgaccgg gtcaccctga ccagccgggc
    25921 cttccacaac gacaagatgg gcatgttcct gaagaagctc tgcgagttca cgggctacga
    25981 gatggccctt cccatgaaca ccggggccga ggccgtggag accgccatca aggccgcccg
    26041 ccgctggggc gtggaggtca agggcgtcca gaacggcaag caggagatca tctgcatgga
    26101 ggacaacttc cacggccgga ccctggccgc catctccatg tccagcgatc ccgattccac
    26161 ggtcaactac ggtccctacg ccccgggctt catccgggtt ccctatcagg acatcaaggc
    26221 catcgaaaaa gccgtcaccc cgaacaccgt ggcggtgatc ctggaaccca tccagggcga
    26281 ggcgggtgtg gtcgttccgc cggagggata cctgaaggcc gtccgcgccc tctgcgacaa
    26341 gaacaagatg ctgttcatcg ccgacgaggt gcagaccggg ttctgccgga ccggcaagcg
    26401 cttcgcctgg gagcacgaga acgcccggcc ggatatcatg tgcctgggca aggccttggg
    26461 cggcggtgtc atgccgatct ccgccatcgt ggcggaccac ggggtcatga aggtcttcac
    26521 tcccggtacc cacggctcca ccttcggcgg caaccccctg gcgggcgccg taggcatcgc
    26581 ctccctggag gtgctggagg aaggcaagct cgaggagaac gccgagcgcc tgggcaggat
    26641 cttccgggac gaggtcgcca agatcccctg caagaagatg gtcctggtcc gtggcaaggg
    26701 tcttctgaac gccatcgtct tcgagaaggg cttcgaggcc tggaacgtct gcctggccct
    26761 ccgggatgcc ggccttctgg ccaagcagac ccacggcaac atcatccgct tcgccccacc
    26821 tctcgtgatc aacgagaagg agcttcggga ggccatcgcc atcatccggt ccgtcctgga
    26881 gaagatctga cggagagctg agcgacgggg cagggttttg taagcttcgg cttcgagcct
    26941 gagtccacag ggggccgtcc gggaggcgcg cgtagcttcc cggacggccg tttttatttc
    27001 gccacgggca ggcgcagctc gaacacggag cccccgcctt cccggggccg gtaggacaga
    27061 gttccgcccg cggtccgggc ccacttcgcg gacagagcca gtccgatccc cgaacccttt
    27121 tccttggtcg tgtggaaggg ctcgaagagc cgggcttcct cctcgggatc caccccgggc
    27181 ccccggtcca ggacggacac gacggcctgg cccctcttga caccgcaggc cagctccacg
    27241 ggcaggtcgg tccttccttc catgctctcc cgggcgttcc gggccaggtt gtccaggatg
    27301 tccgtgagcc taccccggtc gatgcggacc gccgccccct ccgtaacggc gccctccaga
    27361 cggacgcccc ctccgtgccg cttcgcccag ccctccagga attcccccag gtccacggtc
    27421 tcgggccggc cctccccgct ccggaggaaa tcccggatcc ggtccaccag gcctcccagc
    27481 cggcggacct cctcgtcgat gatgcgaaag ccctcctcgg atccgccccc cagggtccgg
    27541 gccaggaggg ctgtctggac ccggatgacc cccagggggt tcttgatctc gtgggccagg
    27601 gtccgggcgg cttccccgag ttgtaccagc tcccggttcc ggtattcccg gtcccttagg
    27661 tcgaggttcc tgcggtacag gtgcacaagg agcgcgtagg cggcccccag ggccgcggtg
    27721 atcgccagtg cgccggcgct cagggcccgc tgttccctcc ggaaggaccc gaggccgtat
    27781 tcgatgtagg cgaacctcgc ggcccctccc atgggcatca tcatgccgct tcccgggcgg
    27841 tccggcctgc cggggacggg cctgcgcagg ggatccattc cgaagggccg gaccaggatg
    27901 aaggacgagt cggtgaacct ccgggcggag gccgggtcct ggagtatctc cggggccagg
    27961 cgatccgggg tccggccgtg ctggtagagc cgggtcccgt ccgaccgata cagcccgaag
    28021 ccgagaatcc tgtcgtccgg gggcatgacg gctccagggt cccggtacat gtccgtcagg
    28081 gccgtcaggg cgcggtagac ctcgtactcc gcgtagagcc tccgtgtgga cctctcccgg
    28141 agcacaaagg cccagaccag gaacacgatg cacagggcgg cagccgcggt tccgaaggtg
    28201 aagatcacgg tccgaatgcg cttcatggca tcccagtata tcccgctcag gacggggcag
    28261 gaaggcgccg cgccgttttt ccgtccttcg tattgcccct cccggcggag ccgggtagaa
    28321 tcgttccatg acgaacgccg agggatccga cctgctccga aaagccctgg aggccggatc
    28381 ccggagggac tacgagtccg cggcggacat cctcacccgc atcgtctccg agacggacga
    28441 gttccccgag gcgctcctct acctgggccg gagcctccac tccctgggcg atttcggaaa
    28501 ggccatcggg gccttccggc tctacctccg tgacacgagc gccaagccgg aaggctggtt
    28561 tttcctgggc cggacctacc tcgccgtctc ccgccccctg gagtccgccc gctgcctgcg
    28621 caaggccttg gagctcgggg cggacggcgc ggaaacctgg tctctcctgg gcttggcggc
    28681 ccttcgcctg cgcaagagca aggcttccgt ggaggccctg gagcgggcgg tcacgatggc
    28741 ccccggcaat ccccgggtgt tccggggcta cctgaacgcc ctcttcgtcc acgccgtgcg
    28801 gaccctgaac cgcggcgacc cggacatggc ccggcagatg ctctccttcg tgatcgccaa
    28861 cggcctggac ggggtccccc agaggctctg gcgcagccga gcctaccggg agctccgccg
    28921 catccccgag gccctggcgg actgccgggc ggccctttcg gagaacccca aggacccggg
    28981 gctcctgacc ctggaggccc tcctgctcct ggccgacggc agtcccgagg acgcggccgc
    29041 ggcatcgacc cgcctccggt ccctgtggcc cgacatggac ggggtcgcct ggaccgagga
    29101 ttccctggag cgttatcgcg ccgttgccct gctgagggac ggcgatcacg cgggcgccct
    29161 gcgggccgcg gtggggatcc tacgcaggga gggtcccgac gcgggcatgc gggcactggc
    29221 cgcggaggcc tgccgcgaac tgggacgctt cgaccgcgcc gtggaacacc ttacgcgggc
    29281 cctggagttg gatcccggga accccgacct cctcttcgag cgggccgtct gccgatggcg
    29341 gctctcggag ttccaggaag cccttgcgga tttcggcagg gcccgcaggg cgggggcgga
    29401 cgcggacgcg gtggagtact ggtccgtcct gtgccgctgc cgactcgggc agggaggtcc
    29461 ctcggtcctg gagtccctcc agaagatgct gcggctccgt cctgcggacc ccccgctcat
    29521 gttcgccctc ggggaagccc tctataagga cggccggccg gacctggccc ggaactggtt
    29581 cgacaggacc ttccaggcgg atccgatgca cgagatggcc gccctctacc ggatatccag
    29641 ccgggaatcc ctgggcgacc gggaaggagc cttcgaggcg tacgcggact acctggagcg
    29701 gtggccggac aactcggaga tccggcggga atacgtcggg gccctgatgg ccgcccggcg
    29761 cttcgccgac gcggccgccg ggatcgaggc ggggatcccc tacgagccgc cgggaaaccg
    29821 agaccacgcc gccttggcgg cctgttaccg caacatcggc cggtaccggg aggccgggac
    29881 cctctatcgc tccctcctgc gggcctcccc gaagaacgag gatttccttc tgggcctggc
    29941 cctgtgcctg gacaaatcgg gctccggcgc caccgccgtc gagctcctgg agaagggagc
    30001 gccctacgtc ggaacgtccg gcccctggtc cgccctgggg atcctgtatg cccgcagggg
    30061 ccgggtcgag cgggccctgg aagccttccg gaaggccgcg gatctcgacc cgcgggatcc
    30121 caagcccctt cgcaacctgg cccggatcta cgagaagacc ggccttgcgg acctggcgaa
    30181 gaaatacaag gcccgagccg ctgcggcgga gagaagctca aagaccccgg aatcccttgc
    30241 caaaccgggc agatagtcta tactcgaacc gttaggagga atgtatgtcc ctaaaagcca
    30301 tggacctctt cgacgcctac gcgaaaagcc agctccccaa ggaccacggc ttcatcgtgt
    30361 cgtccttttt cagcccgaac tccgcctact cccggtacga ggtggtctcg tacaacaacg
    30421 tgaaatccat ctacccctcc ggcgaggggc tcaccttcca gacggacggc aagaagctgt
    30481 tcatcctggt ggaaccccag aactacgcga agaagagcga ggaaccctac gtccgatcca
    30541 gcacggaaca gatccccctc cgcttcagcg agctacactg ccacaccgcc aagaaccaga
    30601 cccgcgtcta ttacggcaag aaggccgtcc tgtcctacgg cgccttcacc atccttaagc
    30661 ccacgggcat caacttctcc ttctgtttct actatcttcc cgatatgttc gattccatgc
    30721 gcatattctt cgagaagacc ttcaacaagg aagccggggt ccccctggcg gacgccaaga
    30781 aggtggccgc ggcggtggtc gagaccgcca agcaggccct ggccgtggag ttcacttcct
    30841 agggcagagt cgaccttccg ggataaaacg gccggccccc tct
//
LOCUS       DAOTEI010000020        30523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_993_length_30523_cov_5.326342, whole genome shotgun sequence.
ACCESSION   DAOTEI010000020 DAOTEI010000000
VERSION     DAOTEI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 30523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30523
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_30523_cov_5.326342"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="P5313_03280"
     CDS             complement(<1..514)
                     /locus_tag="P5313_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HRY79420.1"
                     /translation="MSLPGIPRARVRVLSCGILREELGALPPALLARLDIVILDSILH
                     MRPLDLESGLHGLLDSAPGAPAILVFGDCSPGMREISEGTGLIRIPGVNCCEIVLGRE
                     RYRNLRRQGVFLFMPEWTARWREVFQAELGLGDSETARGFMTDSMKALAYVDTGVVPV
                     PHAILAEIEAF"
     gene            complement(511..1527)
                     /locus_tag="P5313_03285"
     CDS             complement(511..1527)
                     /locus_tag="P5313_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRY79421.1"
                     /translation="MKPVERVTALFRGEPYDRPPYGLACCTLGASLTSTPVGEYYGDP
                     ALYARGQGAVCDLLDPDIVFTPFVFAFEAAAFGAALAPQKDSVPNVRQPPFRTAEDAL
                     TGRDPKPGSDRYLDFLVDSSVACSKAFGTERIVAAPIVAPTDLPALLVGIEEWIETLL
                     FRSDVAERLFSKALDHFRSLSEAYRNAGVSILVMPMMFANPRILDERNIRRSALPVLE
                     SALRDCPLPVVLHHGGTAFADRIGLYTGLPGLAGFAIGAQDSFSRARELAGPEAVLLG
                     NILGPCFDAYSRDFLSGALRALVEAQRGDRRWIFANSGAELPAWTPPDRLTLVRDILQ
                     GRGS"
     gene            complement(1524..2033)
                     /locus_tag="P5313_03290"
     CDS             complement(1524..2033)
                     /locus_tag="P5313_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY79422.1"
                     /translation="MLKAALEDGDPGTAAPPRGVAVLGNIEDDFHALGRKMVASFLRL
                     DGWEIVDLGNDVSPADFVDAALEQKARIIGVSAMMLSTALNIRGLREELDRRNLSGVL
                     PLAVGGAVFNMRPGLVKEVGGDGTASSAMDAPALFRTLCDNAAASGPVSDTESAAGDD
                     AGMPDGART"
     gene            complement(2049..2249)
                     /locus_tag="P5313_03295"
     CDS             complement(2049..2249)
                     /locus_tag="P5313_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79423.1"
                     /translation="MPEYDLEGLLNEILDASLEAALKRLADAARRDGYPAVIRNYLEP
                     VLQRTGKMWIDGTISLAQSYIA"
     gene            2426..3514
                     /locus_tag="P5313_03300"
     CDS             2426..3514
                     /locus_tag="P5313_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY79424.1"
                     /translation="MVRNSRALEELIDHPDTAAGRALDSLFELRPDLRLRYDERSLRL
                     AREDMAHHVRRLAQSALSGEVDSLKDYLSWLKVLFRGLPLPDELIAESLRCAARGAAG
                     SLKDPDARVILELGELAAESYAGLDAYSSRYGGPGPAPGTPVHAYVRSLVDGRRDLAG
                     KVVETELSRGRSVRDLYLDLFQPAQREIGRLWHVREVSVAQEHFATAATQYLMSELYP
                     RLISQSRPNGRVLVAASAQGELHEVGIRMVADFFQSDGWDSRYFGANLPAESLLAEVD
                     RLRPDLIALSATLPENVRWISRVVEALRARGPGRPAVLVGGLPFLVSEGLWRRIGADA
                     TGSDCREALVTGNRIAAERSGPDGKART"
     gene            3511..4122
                     /locus_tag="P5313_03305"
     CDS             3511..4122
                     /locus_tag="P5313_03305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY79425.1"
                     /translation="MTQEELLEEFSRMNNELINTKRELLRLNRELEESSRIDPLTGLR
                     NRRSFDELADQHHKVAARGGGSFAVLYFDLDDFKSVNDRFGHAEGDAALKAAAGILAS
                     CFRAGDILARIGGDEFIALQANTKPGSVDTLTGRIRAAARNWNETSGKLWSIRFSIGG
                     AEFDTATCESLAELIDRADEAMYRDKRGKSRKPDELDGADRPG"
     gene            4162..5157
                     /locus_tag="P5313_03310"
     CDS             4162..5157
                     /locus_tag="P5313_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRY79426.1"
                     /translation="MKRAVLFLSVLILSLAAFAQPKVAVLDAVLQKGIDKSASAAISE
                     KLTERLVLSGRFIVLDRSNVEQVLREREFQLSGLVSDSEISEAGKYLGADFVVAIRVQ
                     RVDRTYMVTAKMIAVDTGIIARQASAEGEGRASILLALAEEAGDGLAGIARKPSATSD
                     RKAAPRPASSARPADAASIGTRLYAGLGTGGQHLEDSLESETYDTIGFDLYGLVGLWK
                     GLCAVGSLSYLDGSAQGGGTALSLDAGLGYARPLGILLPWVALKAGYVFLDWSPYSSV
                     LSDLQYSLDLGADLRLGRLLLGGRLQVSLSAFADSGSYGLTALQNSFWFMAGWKL"
     gene            complement(5294..6244)
                     /locus_tag="P5313_03315"
     CDS             complement(5294..6244)
                     /locus_tag="P5313_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79427.1"
                     /translation="MNAGSFILNILILGVPFSVALLLAGLGETINQRSGVFNLGCEGI
                     MAMGAFLAFLPPYFLAKTPLAPSANLIGILLAVAAGALLGLLFALVVVTFRAPQGIAG
                     IGLQMLGWGTAGTLFRRYVGGVTGVTGIPSLPIPGLSRIPILGPILFNHNPLVYLAFL
                     LVPVFWYILFKTPWGLKVRAVGTYPRAADSLGIPVARVRYQALALGGAMAGLAGAYLS
                     LAQAKMFADDLIAGRGFIAVALVYFGRWNPVGIMGGALLFSLAQSLQLSIQVAGIDFP
                     YEFAVMLPYALVIVVLALVRKARMVGPAELGKPFDREQRV"
     gene            complement(6241..7461)
                     /locus_tag="P5313_03320"
     CDS             complement(6241..7461)
                     /locus_tag="P5313_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79428.1"
                     /translation="MNKTMKAVYNAAVVLAATLAAMGIGALVFAAVGADVVKAYGVIL
                     TEPLKDLIGITEIAVRAVPLTLIALGIAIAFRSGTLNIGAEGQMLMGILASSAAAIAL
                     PDLPKPLLIPLAVLAGAAGGAVWGAAAGILKARLGVNEILSTVMLNYIAAQFYTFLLR
                     GPMMDPEELVTGSGTPQSVRIPRAAWLDRLLPGTRLHAGVYIALVLAVVVFILLWRTS
                     AGFRMRAAGAEAKAARYAGIRVERYVVAAMLLSGAFAGIAGAVEILGVHRRAIEGVSG
                     GYGFTGIVVSLFGGLHPAGILPASLFFGLLLLGADMTQRLADVPANMVLVLQGAIILA
                     IVSARQFMNNPYVQDRLARRLARIRGRPAPNPIPGSPEACPDPSDSPDPCLADEPAAL
                     AGGGSRDVRPEDQR"
     gene            complement(7454..8986)
                     /locus_tag="P5313_03325"
     CDS             complement(7454..8986)
                     /locus_tag="P5313_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79429.1"
                     /translation="MQFEPIRSLELRGITKRFPGILACDHIDLAVGEHSVRALVGENG
                     AGKTTLMNILMGLYQPDEGSILLNGREVRFRSPNDAFAHGIGMVHQHFMLVPNMTVTE
                     NVALGMKSTHRPFRLDLGAARKRIREVSERHELPVNPDAYVWQLSVGEQQRVELVKTL
                     CLGARFLILDEPTSALTPQETDELIELLKKMSRELSVIFISHKLHEVKALSDTVSILR
                     RGRIVFEGRTEDHSPADLAALMTGHEVALPCVECSDAAPAEGRGLLSVRDLRVRGDRG
                     NLALDGLSFDVAAGEILGLAGVSGNGQRELAETLNGLRKPESGSVLLDGAELGGRGAD
                     AVIASGMGYIPEDRNTEGIVPSFSIRENLVLKDVGTVDWSRGGFLRLGPIARNAADLR
                     ERFDIRCPSVDTAAGSLSGGNIQKVILARELARKPKALVAVYPTRGLDMGAEEFIHKQ
                     LLALRAAGVGILLVSEELDEIMNLSDRIAVIYKGRILKVVPGRDATKRSLGLLMAGVL
                     DE"
     gene            complement(9057..10070)
                     /locus_tag="P5313_03330"
     CDS             complement(9057..10070)
                     /locus_tag="P5313_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRY79430.1"
                     /translation="MKKLVALLVALALLAAAGCSRKPEAAPEKPKAKIALIIESTVDD
                     KGWGQAMHDAIAAVQKKYGAALVEYSYSEKMKPVDAGSAARQYASQGFDIIIAHGAQF
                     KNLILELSEEFPKTTFAFGTNADIGPKNVFTYMPMSEETGYINGVIAGMVTKTGIIGV
                     VGPVDAGDSARYNRGFWLGVKAVNPKAEIKVAHTGSFADFVKAGELAHTHVKANADVL
                     TGAAQQATGALRAVAEYKDKPIWWLGQDTLQLTMPEGYKVIAAASYDYSAVVEAMMDK
                     RQAGILGGENIPLTFRNGGFVYKFNDSVGPVLTEAIRKAAEQAKTDITAQTLKLDWQA
                     VKY"
     gene            10260..11372
                     /locus_tag="P5313_03335"
     CDS             10260..11372
                     /locus_tag="P5313_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRY79431.1"
                     /translation="MKVSKVYWTDLRTTPDTPMPVKLDRLLRAAGLADLELRKKLVAL
                     KIHFGEPGNLSYIRPPYVAPVARLVRELGGRPFLTDSNTLYSGRRHDAYDHIRAALEN
                     GFSRDATGCDILIADGLRGNDFVLLPVEGGKHCSAAMIGSAVAEADAVISLNHFKGHE
                     LTGFGGAVKNLGMGCASRAGKLFLHEVSHPSIIQADCTGCGSCVENCAAGAVTLNPEG
                     KAVIDHKLCTGCCQCVAVCGFGAARASEGSASAVCGRRIAEYTAAVVRGKPCFHVNFV
                     MNVSPNCDCWGHNDAPIVADIGIFASPDPVALDQACVDAVNAAPAIGGTVLMDKDYGG
                     SGEKFGHIHPDTDWSATLDHCELLDLGSRAYDLVKV"
     gene            11399..12049
                     /locus_tag="P5313_03340"
     CDS             11399..12049
                     /locus_tag="P5313_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79432.1"
                     /translation="MANRFARRIRNGTIRTPGELKSEFKSLAKALHPDLAGHGAGEEF
                     IRVRSEYEAALRDFELHRFRTRPRRSEGQPAASEAGTDTDARPRVFAALGILRGRGFP
                     KVPRHEKERFRYEYARYRFETALGELGADAGTDLAAVERALLDLRAREPSSFEAVLAH
                     LDGLTEYAARGLPAMRTSLALESHSLGADPGIPETVKRFLGALADTLGLVQALPDT"
     gene            complement(12077..12619)
                     /locus_tag="P5313_03345"
     CDS             complement(12077..12619)
                     /locus_tag="P5313_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRY79433.1"
                     /translation="MNPLELLWAALATAGFAVLFHLRPRDLPLAVVGASLGWWIFSAV
                     RAAGDPAIGYFAAAAAIGFLSELGALVFKKPAFLYMVCSILPLVPGSGMYNTMLQSVR
                     GDFPGSLAIGFSTLQAAGAIAAGLAVSSALARLVSLAAAARRRSVEPPPGPQIGPGSG
                     RGFEDDSGGDARRSGWISAK"
     gene            complement(12616..13395)
                     /locus_tag="P5313_03350"
     CDS             complement(12616..13395)
                     /locus_tag="P5313_03350"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRY79434.1"
                     /translation="MPEPNPEDVLLLASDAARLVLASGAETYRAEETAQAFCSALGGV
                     EAECYATPTGVLLSFFGPDGRSRSIVRRIKGRSMNLERIAGIHALTRDLYAGRAEFHQ
                     AAAGLDALERLSGYPAAVTLAGAAAAAGFFTLLYGGAWNDALVAAPVGALLSRLTRIL
                     SRHGISDFFTNITGGALAALACLAAGLAGWASGPDTAIIGSIMLLVPGVAITSAIRDT
                     IAGDLVAGVARGADAFISAAGISIGAGAAFGLWRLAGLGAL"
     gene            complement(13397..14086)
                     /locus_tag="P5313_03355"
     CDS             complement(13397..14086)
                     /locus_tag="P5313_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79435.1"
                     /translation="MKKFPVVVLVLLLAAQAAFSQSKALDPLWTVSSKSYPAAGKFGV
                     PKSWAPGQYVVVGQLSNGKRDSVTTTLVVAKVGNGWVIENSSVDKAGKESVMQMLLGN
                     YDTAMKTGDASQIELVWIKTLEKDGSVTKTEGQSLIVMKTLMKSSYEKMIVNLSEYVD
                     GGSVQVPAGTFAGTTVVTAKTKILGKTIETESWFHPSVPVNGMVKSRTKDDKSVSELI
                     SFGFDGKPRIP"
     gene            14332..16191
                     /locus_tag="P5313_03360"
     CDS             14332..16191
                     /locus_tag="P5313_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY79436.1"
                     /translation="MKKHGLMFRLNVFLPSLALAAAVIGVYTVFRLPDVPIARILEVC
                     GAAGLAFILATFFLVGGGGKHFAADFAALQKDEPAYETALERLGGTPLLSMIKYLLLS
                     LAFLAGLGAFAGRTFLRDGTGGLVTMFLMALAMFSTSFLYVFADRLVSVTLLSYSLTS
                     FPRDLREARQQRKILIIPAFMAVMTLLFAFSTSLMVTGHSSELTSLSATWILMILSGA
                     YLVVVVLLVLVWNSGTALLYKSVISEMDALSSAEKDLTRRISIASVDELGTISGMVNS
                     FCSTLSESVSGLKAAQARLNELGEGIGVNAAETAQGVERIASHVEKVREKTHAQSASV
                     GESSGAVHEIAKNIESLDQMITDQSASVTEASASIEEMVGNISSISESIEKMAGEFGD
                     LLSAADDGRKKQDQARDKIRQIAERSEALLEANKVIAVIASQTNLLAMNAAIEAAHAG
                     EAGRGFSVVADEIRRLAETSSKQSGAIKAELGRVQEAIREVVDASSASGESFSRVAER
                     IGSTDALVREINSAMEEQKEGTRQILEALQSMNQITSQVQVGSKEMNEGNNTVLAEVS
                     YLQSATLEIKESIEEMASGAEALASGARKVSDIARGARDTIQGMDEELERFRT"
     gene            complement(16248..16565)
                     /locus_tag="P5313_03365"
     CDS             complement(16248..16565)
                     /locus_tag="P5313_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79437.1"
                     /translation="MKALRRIAVVLSIAVLLLVSCAPGPNTLRGTQDEAGKIAGFWKG
                     LWHGAITPVTFVISLFNKDVNVYESRNSGGWYNFGYVIGLSIVFGGGGRGSGGVRRRN
                     RQG"
     gene            complement(16736..17053)
                     /locus_tag="P5313_03370"
     CDS             complement(16736..17053)
                     /locus_tag="P5313_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HRY79438.1"
                     /translation="METSTIRSALAIQCLPLGFPDRERTWAAVDRAIASIEATGLSYT
                     VGPFETVLEGPLDRLLEAAAAAHRALLESGAPTAATYMKLWSGQGIGSSEEKTGKFRA
                     AGH"
     gene            complement(17134..17856)
                     /locus_tag="P5313_03375"
     CDS             complement(17134..17856)
                     /locus_tag="P5313_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRY79439.1"
                     /translation="MRALSNPRNPLAALFVTVCLLAPVLLSTGYATPLAFLVLGLINL
                     RILGGVRYPDYFKTLSVLSLASVGLFVLNVLAPAEGVNGPARGAAVFLRSVSLISLSM
                     GFIFAVDPYDLVRALMVQLGLAPRVGFALFAGWNTIPLLRRDLGIIEKAQALRYGTRR
                     RRFRDRTHTATALLAGAVRHGERVALSMAARGADKPGPRTFLKDSPWTRGDTLYCAAG
                     FLAAALTTALILSLGLYTFDLG"
     gene            complement(17831..19195)
                     /locus_tag="P5313_03380"
     CDS             complement(17831..19195)
                     /locus_tag="P5313_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY79440.1"
                     /translation="MPDLIRLRSAGFRHRGARAPVLKDIDLTVREGDRILIRGPSGSG
                     KSTLLQVMAGLAPEYVPGTLDGIVEHLYASMGVVLQNPEAQVITPTVEEEIAFSLEND
                     GVDARLIRRRTAEVLDRLGIGRLAARHPLTLSGGECQRVSLAAALAREPDILFLDEPT
                     AYLDEASAAAFFRALEDLPARTAVVVVEHKIEAAAAVCARAWEVTPEGGLVPSDFRTP
                     PPPAGSRTGKGSLPSETEPPALEVRGLSHGFEGFPPVFRRVDLDVRPGRVAALTGPSG
                     CGKTTVLARIARILPAGPGSIRLAGRDALALKPRDFHASLLYIPQNPEHMFVAETVRG
                     ELGLSGPEALESADRYGLRNRLDSNPYRLSEGEKRRLNLCVALAERRPLYLLDEPTYG
                     LDEAARESLVRDLGALADSGAGVLLVSHDEGFVGAVADTVFRMKDGRILREEVPRAGA
                     LKSA"
     gene            complement(19188..19763)
                     /locus_tag="P5313_03385"
     CDS             complement(19188..19763)
                     /locus_tag="P5313_03385"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRY79441.1"
                     /translation="MAVKKSWKLNEIVVLVVLSVAIGVLFWGWTFVSALAAPLSSLGL
                     SYLLAGMWFTGGTLVPFLIRKPGAALFGELIAAVLEGFITQWGLTAAIWGLVQGLGCE
                     LVFAATGYRRWDLRVMIAAGMAAGILSYGLDFFYSQYWTLQPWVWPVQIASILAGGAF
                     WAGFGAYRAGRAIIRTGVAANLLSGDDSPLA"
     regulatory      complement(19809..19907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            20009..21595
                     /locus_tag="P5313_03390"
     CDS             20009..21595
                     /locus_tag="P5313_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY79442.1"
                     /translation="MSAAALNLAGLAAGFLYTLVPAVEAAGHFFGWILPGAAAMACAV
                     CSMPRAPRRPSAVYLAWTGLGFAILPFLLIARASFLTPFVFDAASRLVVLGSLGSGFV
                     AAVSRPASGRPDFAGKDRRARPAAAAAGFFVGIAAFAIAARFLTGPHPRTAGVLIPQC
                     LPYWSLSFLAVGAATARSGGGESSTRRGAGLLALPAVAAAILGGMPVYQTPGVLRTAE
                     RDYSRAFEPESATPGRGGSGTAFFPGRMFLGTPAGRIRPILDVPYLAETVPDGRIFTF
                     RYDAWPPNHKGPRPVLIRIHGGAWVSGDKGYGNINNVNRHFASRGYLVFDLQYGLAET
                     GTFRPRSPTPEGMLGPYGIEDMLRHIGRFTTYLADRAEELGADTSRVFVSGASAGGHL
                     ALAVALAGATGYAREHPGSGPDPRITVRGVIPFYPGIGYAASMGIPTPAELDSLEPLL
                     GPANPPALFYQGSLDGMVDPVRVRGFVEAYNRAGGGPAVLVEFPSAGHGSDLIFWNPY
                     GQIFLRYMERFMDRLSGENP"
     gene            21610..22923
                     /locus_tag="P5313_03395"
     CDS             21610..22923
                     /locus_tag="P5313_03395"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRY79443.1"
                     /translation="MNGSTKRGRIAALILLCSVLSAAADVPVPRISGDEVLALAAELS
                     GPLYEGRGIGTEGGRRAAGLLEGMLRKAGYRVQFQEFPERPGFNRGEAGLDVLLPGGA
                     RQTFVYRKDFRETARGGWTGGTVEGPLRLLEARGQEFPGGSVLLIPASLYDPAALDSY
                     AARGAKGVILELPEGSPAQRSGYTGAAPGALVEPKQGMIVLAVSSDAYRRLADAAAGK
                     ARVRMSSPVRFEDARGRNIVAAWNGDGGDFRPTLAFIAHYDHMGTDADGSRFPGALDN
                     ASGTALAVRLAEAIALGSTRADFALVLTDGEEVNLGGAWALARDPPFPLRGVSVVNLD
                     MVGSRADMELSVFHNGDPASRQLADRIAEVVREAGFTAVLRSPVYNLDHGHLPRAGAR
                     AVSVCEFDTSFYHTKEDLPGILSASELSVLGGALLRFALEELESR"
     gene            23054..23242
                     /locus_tag="P5313_03400"
     CDS             23054..23242
                     /locus_tag="P5313_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HRY79444.1"
                     /translation="MAEKETLVIASKVKAYIKEAGDMKCSATVIDALSDKVRALCDEA
                     VKNAKADKRKTVQDKDVR"
     gene            23331..24188
                     /locus_tag="P5313_03405"
     CDS             23331..24188
                     /locus_tag="P5313_03405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY79445.1"
                     /translation="MIEILAVSPPIGDLEIGLRSFQESCGTRDGTIRLETEFALNAFT
                     DMRALFLARDSDRLVGALSVFAPTRHEAEINAAVLPDRRRGGIFRSLLSEAERELARF
                     GYAEELFVVDSGSGPGKEAAARLGGTHEYTEYTLRYAGPCPEVPARDLEIRRVGSESL
                     EDLVDLRSGAFGDSREETEAFERATLASPERQVYAAFLGDRMAGACSLGFQGREVSIN
                     GLVTAVDLRGRGYAQSLLARIVRTLESEGYGMTLDVESRNADALHVYTKLGFLPERAV
                     EYWRRSLPV"
     gene            complement(24185..25207)
                     /locus_tag="P5313_03410"
     CDS             complement(24185..25207)
                     /locus_tag="P5313_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRY79446.1"
                     /translation="MTFQEVREAAKKNIGPHCRVCPECNGVACRGEIPGPGGKGTGAG
                     FIRNYQELRKIRPAMDTIYRSREVSTETRLFGRTFRLPVFAAPIGAVGLHYNDLYTDG
                     TYSAAVVSGCAAEGSAAFTGDGPKDPAFFEPLEAIRAAGGIGIPTIKPWGVREVIERA
                     KKAEDAGVFALAMDIDAAGLTVLAAMGKPVAPMPVEEISRIVESLRVPFILKGIMTPE
                     GARKAVRAGARGIVVSNHGGRVLDETPATIEVLPEIVAAVGGEATILIDGGFRTGLDV
                     FKALALGADGVLIGRPFAYAVYGGGAEGVSTYLRKVRAELEEAMLMTGAAVLSEIDAS
                     KVSRPA"
     gene            25390..26447
                     /locus_tag="P5313_03415"
                     /pseudo
     CDS             25390..26447
                     /locus_tag="P5313_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436366.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
     gene            complement(26536..27588)
                     /gene="asd"
                     /locus_tag="P5313_03420"
     CDS             complement(26536..27588)
                     /gene="asd"
                     /locus_tag="P5313_03420"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRY79447.1"
                     /translation="MERIGVAILGATGIVGQRFVARLAEHPLFEIKYLSASGRSAGLK
                     YGEACTWRAPGRPYGGLADRIVEETSPERAWAPVVFSALDAQAASELEPLYAASGALV
                     FSNASSYRMHEDVPLLVPEANSDHLALLEEQRRRRRWRGGIACNPNCTTAILTTALAP
                     LHRTFGIEAALVTSMQAVSGAGYPGVSALDISGNLIPFIRGEEEKLETETAKILGTLQ
                     GGRVEPAGFPVSATCTRVPVRDGHSVSASVRLAGNPSIEEVVGCLKAYRSPLADLGLS
                     SAPEYPVTVREEADRPQPLLDADAGDGMSVSVGRFRSCGILGLKFLALGHNLERGAAG
                     AAVLNAELWVSAGVPA"
     regulatory      complement(27806..27992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(28120..29424)
                     /locus_tag="P5313_03425"
     CDS             complement(28120..29424)
                     /locus_tag="P5313_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRY79448.1"
                     /translation="MRDTLFENVTAVTMDGDTPVRPDSYVLVSGGRIASVTDSPPPDF
                     SGERIDGRGKVLLPGLVNCHTHIPMTVLRGYADDYDLQTWLTKYIFPVEAKLDGRCVR
                     AGALLGIAEALASGTTSFTDMYYFCDDILAAVEESGIKANVSRGTSLFSEDFSFESHP
                     GCVEMRRLHREWDGRDGGRIRIEASIHAEYTSNPKLWAAVAEYAAENGLGMHVHLSET
                     RKEHEDCIAKYGASPAAILDRYGVWGTRAVAAHCVRVTGDDMDLLAARGVNVAHNPVS
                     NLKLASGIAPVPALLARGINVALGTDSVASNNSHDLFEEIKTAALIHKGASGDPRAVS
                     ALDALRLATFNGARAQGRADECGRMVPGMDADLILLDFDRPHLVPCHNVVSNLVYSAR
                     GSDVVLTMVRGRILYRDGAFTTIDLPRVRRELEDYVLPTLFG"
     gene            complement(29435..30199)
                     /locus_tag="P5313_03430"
     CDS             complement(29435..30199)
                     /locus_tag="P5313_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HRY79449.1"
                     /translation="MNQAIIGGTGVYKLFDDKESVTVDTRYGSADVDLVTIDGFRFAF
                     LPRHGRGHSVPPHRINYRANIQALKDLGIRFIYATAAVGSCSERFAPGDVVVLRDFLD
                     FTKGRPSTFFEDGTEVRHVPMGEPYCRKLTEAFEETARDHRLWIKGSAVYVCTEGPRF
                     ETAAEIRMYARMGGEVVGMTSVPEVVLAREAGICYASVGIVTNMCTGLSEEEATLRQV
                     QEAMSRNRAAILRACVDTFKRPMTKTCCEVPAAARV"
     gene            30259..>30523
                     /locus_tag="P5313_03435"
     CDS             30259..>30523
                     /locus_tag="P5313_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79450.1"
                     /translation="MKIIVGHSNMDLDCIGSLVLAQYLFPDHVPLRSHLMQPAARNLM
                     NLYADRLDFPGIKDIKGEKVERVVVVDARSEDRIAEVLPLIEPG"
BASE COUNT         4977 a        10172 c        10444 g         4930 t
ORIGIN      
        1 agaaggcttc tatctccgcg aggatggcat gcggaaccgg cacgaccccg gtgtccacgt
       61 acgccagggc cttcatggaa tcggtcatga atccccgggc tgtttcggaa tcgccgagtc
      121 caagctccgc ttggaagacc tcgcgccacc gggcggtcca ttccggcatg aagaggaaca
      181 ctccctgcct gcggaggttc ctgtaccgtt cccggcccag gacgatctcg cagcagttga
      241 ccccggggat gcggatgagg ccggtcccct cgctgatctc ccgcatcccg ggggagcagt
      301 ccccgaacac caggatcgcg ggggcgccgg gggcggaatc caacaggccg tgcaggccgg
      361 actccaggtc cagcggccgc atatgtagga tcgaatcgag gatcacgatg tccaggcgcg
      421 ccagcaaagc gggcgggagg gccccgagtt cctcccggag tatcccgcag gagaggaccc
      481 ggacccgggc ccgcgggatt ccgggtaggc tcacgagccg cgcccctgca ggatgtcgcg
      541 gacgagggta agccggtccg ggggggtcca ggcgggaagc tccgccccgc tgttcgcgaa
      601 aatccaccgc cggtcacccc tctgtgcctc caccagggcc ctgagggcgc cggagagaaa
      661 atcccgggaa taggcgtcga agcaggggcc caggatgttg cccaggagga ccgcctccgg
      721 ccccgcgagt tcccgggccc gggagaagct gtcctgggcc ccgatggcga atcccgcaag
      781 accgggcagg ccggtataaa ggccgatcct gtcggcgaac gcggttcctc cgtgatggag
      841 aactacgggc agggggcaat cccggagcgc cgactccagg accggcaggg ccgacctgcg
      901 gatgttcctc tcgtccagga tgcggggatt cgcgaacatc atcggcatga ccaggatgga
      961 tacccccgcg ttccggtagg cttcggaaag ggaccggaaa tgatcgagcg ccttcgagaa
     1021 cagcctttcc gccacgtcgg accggaagag cagggtttcg atccattcct cgatcccgac
     1081 caggagggcg ggcaggtccg tgggtgcgac gatgggggcc gccacgatcc gttccgtccc
     1141 gaaggccttc gagcaggcca cggaagagtc gaccaggaaa tccaggtacc gatcggaacc
     1201 gggcttggga tcccggcccg tcagggcgtc ctccgcggta cggaagggag gctggcgtac
     1261 gttgggcacc gagtccttct ggggagccag ggccgcgccg aaggctgcgg cctcgaaggc
     1321 gaacacgaag ggggtgaaca cgatgtccgg gtccagcagg tcgcagacgg ccccctgacc
     1381 ccgggcatac agggccgggt ccccgtagta ttccccgacg ggggtcgagg tgagggaagc
     1441 ccccaaggta cagcaggcca gtccgtaggg aggccggtcg tagggctccc cgcggaacag
     1501 ggccgtcacc cgttccacgg gtttcatgtc cgggctccgt ccggcattcc ggcatcatcc
     1561 ccggcagcgc tctcggtatc gctgacggga ccgctggcgg cggcattatc acagagtgtc
     1621 cggaacaggg cgggggcgtc catggcgctc gaggcggtgc cgtcccctcc gacctccttc
     1681 acgagaccgg ggcgcatgtt gaacaccgcc cctcccacgg ccaggggaag gaccccggac
     1741 aggttccgcc ggtcgagttc ctcccgcagg ccccggatgt tcagggccgt ggacagcatc
     1801 atggcggaaa ccccgatgat ccgggctttt tgttccaggg cggcatccac gaaatccgcg
     1861 ggggagacgt cgtttcccag gtccacgatc tcccagccgt ccaggcgcag gaaggacgcg
     1921 accatctttc ggcccagggc gtggaagtcg tcctcgatgt tgccgagcac ggccactccc
     1981 cgtggaggcg ccgcggtgcc cgggtccccg tcttccagcg cggccttgag cacgttctcc
     2041 gcaacctttc aggcgatata gctctgggcc agggagatcg tgccgtcgat ccacattttg
     2101 cccgtccgct gaagtacggg ttccaggtaa tttcggatca ccgcggggta tccgtccctc
     2161 cgggcggcgt ccgcgaggcg tttcaaggcg gcctccaggc tggcgtccag gatctcgttc
     2221 agcaagcctt caaggtcgta ctcaggcatg tcctctccaa taggatgatg cttccgttga
     2281 tgtaagtatg aaggagtttc ccgaaatgtc aaaaccgccc aggtccggcc gcttgtcttt
     2341 cccctccacc gacatatact tagcgcaaga cttagattac cctgagcggt aaccttcacc
     2401 ttcgatcccc ccggagaggc aggagatggt ccggaattcc cgagcccttg aagagttgat
     2461 cgatcacccc gatacggccg ccgggcgggc cctggactcg ctcttcgaac tccggccgga
     2521 cctgaggctc cgctacgacg agaggagcct ccgcctggcc cgggaggaca tggcccacca
     2581 tgtccgaagg ttggcccagt cggccctgtc cggagaagtc gattccctca aggactacct
     2641 ttcctggctc aaggtcctgt tccgcggcct tccgttgccc gacgaactga tcgcggaatc
     2701 cctccgttgc gccgcccggg gggccgccgg ttcgttgaag gatcccgacg cccgcgtgat
     2761 cctcgaactc ggggagctcg ccgcggaatc ctacgcgggt ctggacgcgt attcgagccg
     2821 gtacggaggg ccggggcccg ctccgggcac ccccgtccac gcctacgtcc ggtccctggt
     2881 ggacgggcgg cgggacctgg ccgggaaggt ggtcgaaacc gaactctccc ggggccgttc
     2941 ggtccgggac ctctacctgg acctcttcca gcccgcccag cgggagatcg gccgactctg
     3001 gcatgtccgc gaggtttccg tcgcccagga gcatttcgcg accgccgcca cccagtacct
     3061 catgtccgag ctgtatcccc gcctaatttc ccagtcccgg ccgaacggcc gggtcctggt
     3121 agcggcctcc gcccaggggg agctgcacga ggtcggcatc cgcatggtgg cggacttctt
     3181 ccagtccgac ggctgggatt cccggtactt cggggcgaac ctccccgcgg agtccctgct
     3241 cgccgaagtg gatcgtctgc gtcccgacct gatcgccctg tccgcgaccc tcccggagaa
     3301 cgtacgatgg atctcccggg tcgtggaggc cctgcgggcc cggggacccg gccgtcccgc
     3361 cgtcctggtc gggggccttc cgttcctggt ctccgaaggg ctctggcgtc ggatcggtgc
     3421 ggacgccacc ggctcggatt gccgggaagc cctggtaacc ggcaaccgga tcgccgccga
     3481 gcggtccggc ccggacggga aggcccggac gtgacccagg aagaattgct ggaagagttt
     3541 tcccggatga acaacgagct catcaacacc aagcgggaac tcctgcgcct gaaccgggag
     3601 ctcgaggaat catcccggat cgaccccctc acgggcctaa ggaaccgacg gtccttcgac
     3661 gagctggcgg atcagcacca caaggtcgcc gcccgggggg gaggctcctt cgcggttctg
     3721 tacttcgacc tggacgactt caagagcgtc aacgaccggt tcggccacgc cgagggcgac
     3781 gcggccctga aggccgcggc ggggatcctg gcctcctgct tccgggccgg ggatatcctg
     3841 gccaggatcg gtggggacga gttcatcgcc ctgcaggcga acacgaaacc tggttccgtg
     3901 gataccctga cggggcggat ccgggcggcc gctcggaact ggaacgaaac gtccggcaag
     3961 ctctggagca tacggttttc cataggcggc gccgagttcg acaccgcgac ctgcgagagc
     4021 ctggcggagc tgatcgaccg ggcggacgag gccatgtacc gggataagcg gggaaagagc
     4081 cgaaaacccg acgaacttga cggtgcggac cgccccgggt agagtggtcc cgggcgcgca
     4141 cgccgcagcc aggagccgag catgaaacga gctgtcctct tcctctccgt ccttattctc
     4201 tcccttgccg ccttcgccca gcccaaggtg gccgtgctgg acgcggtatt acagaagggc
     4261 atcgacaagt ccgcctccgc ggcgatctcg gaaaagctga cggagcggct cgtcctgtcc
     4321 ggccgcttca tcgtgctgga ccggtcgaac gtcgagcagg tcctgcggga gagggagttc
     4381 cagctctccg gcctggtctc ggactccgag atctccgagg ccgggaaata cctgggggcg
     4441 gatttcgtgg tcgcgatccg ggtccagcgc gtcgaccgta cctacatggt caccgccaag
     4501 atgatcgccg tggacacggg gatcattgcc cgtcaggcct ccgcggaggg ggagggccgt
     4561 gcctcgatcc tgctggccct ggcggaagag gccggggacg gcctcgcggg catcgcgcgc
     4621 aagccctccg cgacctccga ccggaaggcg gcgcccaggc ccgcatcgtc cgcccggccg
     4681 gccgacgcgg cctcgatcgg cacccggctc tacgcggggc tcggcacggg ggggcagcat
     4741 ttggaagata gcctcgagtc cgagacctac gataccatcg ggttcgacct ctacggcctt
     4801 gtcggccttt ggaaggggct ctgcgcggtc ggcagcctga gctatctgga cggttccgcc
     4861 cagggcggtg gaacggccct gtccctggat gcgggcctgg gctatgcccg tcccctcggg
     4921 attctccttc cctgggtcgc cctcaaggcg ggctacgtct tcctggattg gtcgccctac
     4981 tcctcggtcc tctcggacct gcagtacagc ctggacctcg gcgcggacct ccggctgggc
     5041 cgcctgctcc tgggcggccg cctgcaggtc tccctgtccg cgttcgcgga ttccgggagc
     5101 tacgggctca ctgcccttca gaactccttc tggttcatgg cgggctggaa gctctgacct
     5161 agcaggctgt tgacaaaggc gatggcgcct ttgtcaacag cttcctcaat gtcgcaattg
     5221 cgtagtaaag cgcaagcgtt tcgagcaatt gcatagcagg gtgctgacaa gcttcgagtt
     5281 agtcagcacc ctgctagacc cgctgctccc ggtcgaaggg cttgcccaat tcagcggggc
     5341 cgaccatccg cgccttgcgc accagggcca gcaccacgat gaccagggcg taggggagca
     5401 tgaccgcgaa ctcgtagggg aagtcgatcc ccgccacctg gatggatagc tggagggact
     5461 gggccaggct gaacaggagg gcgcctccca tgatcccgac gggattccat cggccgaagt
     5521 agaccagggc cacggcgatg aagccccggc ccgcgatcag gtcgtcggcg aacatcttgg
     5581 cctgggccag gctgaggtag gcccccgcca gccccgccat ggcgccgccc agggccaggg
     5641 cctggtaccg cacgcgggcc acggggattc ccagggagtc cgcggcccgg gggtaggttc
     5701 ccaccgcccg caccttcagg ccccaggggg tcttgaacag gatgtaccag aacaccggca
     5761 ccagcaggaa ggccaggtag accaggggat tgtggttgaa caggatggga cccaggatcg
     5821 gtatgcggga aagccccggt atggggaggg aggggattcc ggtgaccccg gtcacgccgc
     5881 ccacgtatct ccggaacagg gtgcccgcgg tgccccagcc gagcatctgc aggccgatgc
     5941 ccgcgatccc ctggggggcc cggaaggtca caacgaccag ggcgaacagg agccccagca
     6001 gggcgcccgc cgctacggcc aggaggatgc cgatcaggtt ggcggaaggc gccagggggg
     6061 tcttggccag gaagtagggc ggcaggaagg ccaggaaggc ccccatggcc atgatgccct
     6121 cgcagcccag gttgaagacc ccggagcgct ggttgatcgt ctcgccgagg ccggccagga
     6181 ggagggccac ggagaaggga acccccagga tcaggatgtt caggatgaag gagccggcgt
     6241 tcatcgttga tcctccggtc gtacgtcgcg ggaaccgccc ccggccagcg ccgcgggctc
     6301 gtcggccagg caggggtccg gggaatccga aggatccggg caggcctccg gggaacccgg
     6361 aatcggattc ggagccggcc ggccgcggat ccgggcgagc ctgcgggcca gccggtcctg
     6421 gacgtaggga ttgttcatga actgccgggc ggagacgatg gccaggatga tggccccctg
     6481 caggaccagg accatattgg ccgggacgtc cgccagccgc tgggtcatgt ccgcccccag
     6541 gagcaggagc ccgaagaaga gggaggcggg caggatcccc gcggggtgga gccccccgaa
     6601 cagggacacc acgatgcccg tgaacccgta gcccccggaa accccctcga tggcccgtcg
     6661 gtggaccccc aggatctcca cggctcccgc gatgcccgcg aaggctccgg acaggagcat
     6721 ggccgccacc acgtagcgct ccacccggat gcccgcgtag cgcgcggcct tggcctcggc
     6781 gccggcggcc cgcatccgga agcccgcgga ggttcgccac aggagtatga acaccaccac
     6841 ggccaggacc agggcgatat agactcccgc gtggagccgg gttcccggga ggagccggtc
     6901 tagccaggcg gcccggggga tccgcacgct ctggggggtc ccggaaccgg tcaccagctc
     6961 ctcgggatcc atcatgggtc cccggagcag gaaggtgtag aactgggcgg cgatgtagtt
     7021 gagcatgacc gtggacagga tctcgttcac ccccagccgg gccttcagga tgcccgccgc
     7081 ggctccccag acggccccgc ccgcggctcc ggccaggacg gccaggggga tcaggagggg
     7141 cttcggaagg tccggcaggg cgatggcggc ggcgctggag gcaaggatgc ccattagcat
     7201 ctgcccctcg gccccgatgt tcagggttcc gctccggaag gcgatggcga tccccagggc
     7261 tatcagggtc aggggaacgg cccgtaccgc gatctccgtg ataccgataa gatccttcag
     7321 gggttccgtg aggatcaccc cgtaggcctt cacgacgtcc gctccgacgg ccgcgaaaac
     7381 cagggccccg atgcccatgg cggccagggt cgccgccagg acaacggcgg cgttgtagac
     7441 cgccttcatc gtcttattca tcgagcactc cggccatcaa aagccccagg gaccgcttgg
     7501 tcgcgtcccg tcccggaacc accttcagga tcctgccctt gtagatgacc gcgatccggt
     7561 ccgacaggtt catgatctcg tccaattcct cggataccag gaggatcccc accccggcgg
     7621 ccctcagggc caggagctgc ttgtggatga actcctcggc gcccatgtcc agcccccggg
     7681 tggggtatac ggcgacgagg gccttgggct tccgagccag ttcccgggcc aggatcacct
     7741 tctggatgtt gccccccgaa agggatcccg cggccgtatc gaccgaggga cagcggatgt
     7801 cgaaccgttc ccgcaagtcc gcggcgttcc gggctatcgg acccaggcgc agaaagcccc
     7861 cccggctcca atcgaccgtc cccacgtcct tgagcacgag gttctcccgg atcgaaaagg
     7921 aggggacgat tccctcggtg ttccggtcct cgggaatgta gcccatcccc gaggcgatca
     7981 cggcgtccgc cccgcggccc ccgagttcgg cgccgtccag gaggacggac ccggactccg
     8041 gcttccgaag gccgttcagg gtctccgcca actcccgctg gccgttgccg gagactcccg
     8101 ccaggccgag gatctccccc gcggccacat cgaagctaag cccgtccagg gccaggttgc
     8161 cccggtctcc ccgcacccgg aggtcccgga cggaaaggag tccccggccc tcggccggtg
     8221 ccgcgtccga gcactccacg cagggcaggg cgacctcgtg gcccgtcatg agggccgcca
     8281 ggtccgcggg ggaatggtcc tccgtgcgcc cctcgaagac gatgcggccc ctgcgaagga
     8341 tggagaccgt gtcggacagg gccttcacct cgtggagctt gtggctgatg aagatgaccg
     8401 agagttcccg agacatcttc ttgagcagct cgatgagttc gtccgtctcc tggggggtca
     8461 gggcggaggt gggctcgtcc aggatcagga agcgggcgcc caggcagagg gtcttaacca
     8521 gctcgacccg ctgttgttcc cctacggaaa gctgccagac gtaggcgtcg ggattcaccg
     8581 ggagctcgtg gcgctccgag acctcccgga tgcgcttccg ggcggctccg agatccagac
     8641 ggaagggcct gtgggtggat ttcatcccaa gcgcgacgtt ttccgtgacc gtcatgttgg
     8701 gcaccagcat gaagtgctgg tgcaccatcc cgatcccgtg ggcgaaggcg tcgttggggg
     8761 accggaagcg gacttcccgg ccgttgagga ggatgctccc ctcgtccggc tggtatagcc
     8821 ccatgaggat gttcatgagg gtcgtcttgc ccgccccgtt ttcccccacc agggctcgca
     8881 cggaatgctc gcccaccgcc aggtcgatgt ggtcgcaggc cagtatcccc gggaaacgtt
     8941 ttgtgatgcc ccgaagctcg aggctgcgga tgggttcgaa ctgcatgaag actcccgacg
     9001 ccggaatctg accggtccgg ggagcgggtc ccccgggccg gtcgtcgatc tacggattaa
     9061 tacttcacag cctgccagtc gagcttgagc gtctgggcgg tgatgtcggt cttggcctgt
     9121 tcggccgcct tgcggatggc ctcggtcagg acggggccca cggagtcgtt gaacttgtat
     9181 acgaacccgc cgttccggaa ggtgaggggg atgttctccc cgcccagaat cccggcctgc
     9241 cgcttgtcca tcatggcctc cacgaccgcg gagtagtcgt agctggccgc ggcgatgacc
     9301 ttgtagccct cgggcatggt cagctggagg gtgtcctgac cgagccacca gatgggcttg
     9361 tccttgtact cggcgaccgc gcggagggcg ccggtggcct gctgggcggc gcccgtgagg
     9421 acgtcggcgt tggccttgac gtgggtatgg gccagctctc cggccttgac gaagtccgcg
     9481 aaggagccgg tgtgggccac cttgatctct gccttgggat taacggcctt gacgcccagc
     9541 cagaacccgc ggttgtaccg ggcggagtcc cccgcgtcta cggggccgac gacgccgatg
     9601 atccccgtct tggtcaccat gccggcgatg accccgttga tgtaccccgt ctcctcgctc
     9661 atgggcatgt aggtgaagac gttcttgggg ccgatgtccg cgttcgtgcc gaaggcgaag
     9721 gtggtcttcg ggaactcctc ggacagctcg aggatgaggt tcttgaactg ggcgccgtgg
     9781 gcgatgatga tgtcgaaacc ttgggaggcg tactgccggg ccgcggagcc cgcgtccacg
     9841 ggcttcatct tctcgctgta gctgtactcc accagggcgg cgccgtactt cttctgcacc
     9901 gcggcgatgg cgtcgtgcat ggcctgtccc cagcccttgt cgtccacggt ggactcgatg
     9961 atcaaggcga ttttagcctt gggcttttcg ggtgccgcct caggcttgcg cgaacagcct
    10021 gccgctgcca gcaaggccaa ggcgacgagc agggccacga gtttcttcat gaaagcctcc
    10081 taaacacgat tcgacagatt gcgtccgcgg gcccggcacc gggctccgcg gatttcggga
    10141 aagaatacca tgggccgatc gggccgtaaa caagaatcga atcgcaagat gttccgatac
    10201 cgggtctttt cggatatagt gtccgggctt gataatcgaa ttcagcggag gccctgcgaa
    10261 tgaaggtttc caaggtatac tggacggatc tgcgcaccac ccccgacact cccatgcccg
    10321 tcaaactcga ccggctcctg cgggccgcgg gactcgcgga cctggagctc cggaagaaac
    10381 tggtagccct caagatccac ttcggcgaac ccggcaatct ttcctatatc cgtcccccct
    10441 acgtcgcccc cgtggcccgg ctcgtccggg aactgggggg ccggcccttc ctgacggact
    10501 cgaataccct ctactccgga cgcaggcacg acgcctacga ccatatccgc gcggccctgg
    10561 agaacggatt ctcccgggac gccacgggct gcgacatcct gatcgcggac ggcctgcgag
    10621 ggaacgattt cgtccttctg cccgtcgagg gagggaagca ctgctccgcg gcgatgatcg
    10681 ggagcgccgt ggccgaggcg gacgcggtca tttccctgaa ccatttcaag gggcacgagc
    10741 tgaccgggtt cggcggggcg gtcaagaacc tgggtatggg ctgcgccagc cgggccggaa
    10801 aactgttcct ccacgaggtc tcccacccct cgatcattca ggcggactgc acgggctgcg
    10861 gaagctgcgt cgagaactgc gcggcgggcg cggtgaccct gaatccggaa ggcaaggccg
    10921 tcatcgacca caagctgtgc acggggtgct gccagtgcgt ggcggtatgc ggcttcgggg
    10981 cggcccgggc ttcggaaggg tccgccagcg ccgtctgcgg ccggcggatc gccgaataca
    11041 cggccgccgt ggtccggggc aagccctgct tccacgtgaa cttcgtgatg aacgtctcgc
    11101 ccaactgcga ctgctggggc cacaacgacg cgccgatcgt ggccgatatc gggatcttcg
    11161 cgtccccgga tcccgtagcc ctggaccagg cctgcgtgga cgccgtgaac gccgcaccgg
    11221 cgatcggcgg caccgtcctc atggacaagg actacggcgg atccggggag aagttcggcc
    11281 acatccaccc cgacacggac tggagcgcca ccctggacca ctgtgagctt ctggatctgg
    11341 gcagccgcgc ttacgacctc gtgaaggtct aggcccggcg gaccggacgg ttccctccgt
    11401 ggccaaccga ttcgcccggc gcatccggaa cgggaccatc cggactcccg gggaattgaa
    11461 gtcggagttc aaatccctcg ccaaggccct gcatcccgat ctcgcggggc acggggccgg
    11521 cgaggaattc attcgggtcc ggtcggagta cgaggccgcg ctccgggatt tcgaactcca
    11581 ccggttccgg acccgcccgc gacggtccga gggtcagccg gccgcctcgg aggcgggtac
    11641 ggatacggac gcacgcccgc gggtcttcgc cgccctgggg atcctccgcg gacggggctt
    11701 ccccaaggta ccccgtcacg agaaggagag gttccgctac gagtacgccc ggtaccgctt
    11761 cgagacggcc ctgggggaac tcggggcgga tgcggggacg gacctggcgg ccgtggaacg
    11821 ggccctgctc gaccttcgcg cccgggaacc ttcctccttc gaggccgtcc tcgcgcacct
    11881 ggacggattg acggagtatg ccgcccgcgg ccttccggcc atgcgcacgt ccctggccct
    11941 ggagtcccac tcactggggg cggatcccgg aatcccggaa acggtcaaac ggttcctggg
    12001 ggcgctcgcg gatacgctgg ggcttgtgca ggccctcccg gatacctgat ccgcggtcct
    12061 ccggacaggg gtttccctac ttggcgctga tccagccgct ccggcgggca tcgccgccgg
    12121 aatcgtcctc gaagcctctc ccggagcctg ggccgatctg cggaccgggc gggggctcga
    12181 cggatcggcg gcgggccgcg gcggccaggg ataccaggcg ggccagggcg ctggagacgg
    12241 ccagccccgc cgcgatggcc cccgccgcct ggagggtgga gaacccgatc gccaggctcc
    12301 ccgggaagtc cccgcggacg gactgcagca tggtgttgta catcccgctc ccgggtacca
    12361 ggggcaggat ggagcagacc atgtagagga aggccggctt cttgaagacc agggctccca
    12421 gttcggagag aaaaccgatg gccgccgcgg cggcgaagta gccgatggca ggatcccccg
    12481 ccgcccgtac cgcggagaag atccaccatc ccagggaggc tccaaccacg gccaggggca
    12541 ggtcccgggg ccggaggtgg aagaggacgg cgaatcccgc ggtggccagg gcggcccaca
    12601 ggagctccag ggggttcaca gggcccccag tccggccagc cgccacagcc cgaaagccgc
    12661 cccggccccg atggaaattc ccgccgcgga gatgaacgcg tccgcgcccc gggcgacgcc
    12721 ggccaccagg tcccccgcga tcgtatcccg gatggcgctc gtgatggcca ccccgggaac
    12781 caggagcatg atggagccga tgatcgccgt gtccgggccg gaggcccacc cggccagtcc
    12841 ggccgcgagg caggccaagg cggccagggc tccccccgtt atgttcgtga aaaaatccga
    12901 aatgccgtgg cgggagagta tgcgggtaag ccgggagagc agggctccca cgggcgccgc
    12961 gaccagggcg tcgttccagg cccccccgta caggagggtg aagaatccgg cggcggccgc
    13021 ggccccggcc agggtgacgg cggccggata gccggacagg cgctctaggg cgtccaagcc
    13081 tgcggcggcc tggtggaatt ccgccctgcc cgcgtagagg tcccgggtca gggcgtgtat
    13141 gccggcgatc cgttccaggt tcatgctgcg ccccttgatc ctgcgcacga tcgagcggga
    13201 ccggccgtcg gggccgaaga aggagagcag gacccccgtg ggcgtcgcgt agcattccgc
    13261 ctcgactccc cccagggcgg aacagaaggc ctgggcggtt tcctcggccc ggtacgtctc
    13321 cgcgccgctg gccagaacca ggcgcgcggc gtcggaggcc aggagcagca cgtcctccgg
    13381 attcggttcg ggcatctcag ggaatccgcg gtttcccgtc gaacccgaag gagatgagct
    13441 ccgacacgga cttgtcgtcc ttggtccggg atttcaccat gccgttgacc ggcacggagg
    13501 ggtggaacca ggattcggtc tcgatcgtct tgcccaggat cttggtcttg gcggtcacga
    13561 ccgtggtacc cgcgaaggtg cccgcgggaa cctggaccga gcccccgtcg acgtattcgg
    13621 agaggttcac gatcattttc tcgtaggagg acttcatcag ggtcttcatg acgatgaggg
    13681 attggccctc ggtcttcgtg acggaaccgt ccttttccag ggtcttgatc cagaccagtt
    13741 cgatctggga ggcgtccccg gtcttcatcg cggtgtcgta gttgcccagc agcatctgca
    13801 tgacgctttc cttgccggcc ttgtcgacgc tcgagttctc gatgacccag ccgttgccga
    13861 ccttggccac gactagggtc gtggtgacgg aatcgcgctt gccgttcgag agctggccga
    13921 cgaccacgta ctggccggga gcccaggact tgggaacgcc gaacttaccc gcggcgggat
    13981 aggatttgga tgaaacggtc cagagcggat cgagagcctt ggactgggag aaggcggcct
    14041 gggcggccag caggagcacg agcacgacga ccggaaattt cttcatgatt gcctccgcca
    14101 gtgactctat cacgcccgcc gtccattttc gagcgccctt gcacggcggg agtcaacggg
    14161 gccccgagtc cggattatcc gagagttttt cccgacggcc tggaaattct gcgcaaagta
    14221 acaatactat cttcaggacc ggtgttcgcg gcagccgggg cgctccggac agggagtcgt
    14281 ccaggatgcc tcggactaga tccttagaac atccatacca tggggttccg catgaaaaag
    14341 cacggcctga tgtttcgcct gaacgtgttc ctgcccagcc ttgccctggc ggccgcggtg
    14401 atcggtgtct ataccgtgtt ccggctgccc gacgtgccga tcgcccggat actggaagtc
    14461 tgcggcgccg cgggcctggc cttcatcctg gcgacgttct tcctggtcgg gggcggcggg
    14521 aagcacttcg cggcggactt cgccgccttg cagaaggacg agccggccta cgaaacggcg
    14581 ctggaacgtc taggcgggac tccgcttttg agcatgatca agtaccttct cctgagcctg
    14641 gcctttctgg ccgggctggg ggcgttcgcg ggaaggacct tcctgcggga cggaaccggc
    14701 ggtctggtga ccatgttcct catggccctg gcgatgttca gtacctcctt cctgtacgtc
    14761 ttcgcggacc gcctggtttc cgtcaccctg ctctcgtatt ccctgacctc ctttccccgg
    14821 gatcttcggg aggcccggca gcagaggaag atcctgatca ttccggcctt catggccgtg
    14881 atgaccctcc tgttcgcctt ctccacgtcc ctgatggtaa ccggacactc ctcggaattg
    14941 acctcgcttt ccgcgacgtg gatcctgatg atcctgtccg gggcgtacct cgtcgtcgtg
    15001 gtcctgctcg ttctggtctg gaattccggg acggccctgc tgtacaagtc ggtgatctcc
    15061 gagatggacg ccctgtcctc cgcggaaaag gacctgaccc gaagaatttc catcgcctcg
    15121 gtggacgagc tgggcacgat ttccggaatg gtcaattcct tctgctccac cctgtccgaa
    15181 agcgtctcgg gactgaaggc cgcccaggcg aggctcaacg aactgggcga gggcatcggg
    15241 gtgaacgcgg ccgaaaccgc ccagggcgtg gagcggatcg cttcccacgt ggaaaaggtc
    15301 cgggagaaga cccacgccca gtccgccagc gtcggggaat cctccggtgc ggtgcacgag
    15361 atcgccaaga acatcgaatc cctggaccag atgataacgg accagtccgc cagcgtgacc
    15421 gaagcgtccg cctcgatcga ggagatggtc ggcaacatat cctccatctc cgagtcgatc
    15481 gagaagatgg ccggggaatt cggggacctc ctgtcggcgg cggacgacgg ccggaagaag
    15541 caggaccagg cccgggacaa gatccggcag atcgcggagc gctcggaggc cctcctggag
    15601 gccaacaagg tgatcgcggt gatcgcctcc cagacgaacc tcctggccat gaacgcggcg
    15661 atcgaggccg cccacgccgg cgaggcggga cggggctttt ccgtggtggc cgacgagatc
    15721 cgcaggcttg cggagacttc ctccaagcag tccggcgcca tcaaggcgga gctcggacgg
    15781 gtgcaggagg ccatccgcga ggtcgtggac gcgtcctccg cctcgggaga gtccttctcc
    15841 cgggtggccg agaggatcgg cagcaccgac gccctggtcc gggaaatcaa ctccgccatg
    15901 gaggagcaga aggagggcac ccgtcagatc ctggaagccc tgcagtccat gaaccagatc
    15961 acctcccagg tccaggtcgg ttccaaggag atgaacgagg gcaacaacac ggtcttggcg
    16021 gaggtttcct acctgcagtc cgcgaccctg gagatcaagg agagcatcga ggagatggcc
    16081 tcgggcgcgg aggctctggc ctcgggggcc cggaaggtgt cggacatcgc ccggggcgcc
    16141 cgggacacca tccaaggaat ggacgaggaa ctcgagcgct tccggacttg aagaagccgc
    16201 ggcgaagtcc gccgcggcgg aaaaacaggg cccggtggcc ggtcggctca gccctgccgg
    16261 tttctccgcc ggactcctcc ggatcccctt cctcctccgc cgaagacgat ggacaggccg
    16321 atgacgtacc cgaagttgta ccagcccccg gagttgcggc tttcatagac gttcacgtcc
    16381 ttgttgaaca gggagatcac gaaggtcacg ggggttatgg cgccgtgcca gagccccttc
    16441 cagaatccgg cgatcttgcc ggcctcgtcc tgggtccccc gaagggtgtt gggtccgggg
    16501 gcacaggata cgagcaacaa gaccgcgatg gacagaacga cggcgatacg tctgagcgct
    16561 ttcatgagcc tctccttggg acggccggtg tttccggccg ggatgttccg cggatcctcg
    16621 tcgatggagc gatccgtgtc ggccgagggc ccgagccccg ttccggccgc gcttggacca
    16681 gtatatcacc cgggcacccg gcgcgccatt cgcccgccgg tatactccgg cccgttcagt
    16741 gtcccgcggc ccggaatttt ccggtcttct cctcgctcga gccgattccc tggccgctcc
    16801 acaacttcat gtaggtggcc gcggtggggg cgcccgattc caggagtgcc ctgtgcgcgg
    16861 ccgccgcggc ttccagcagg cggtccaggg ggccctcgag gaccgtctcg aagggaccga
    16921 cggtatacga taggccggtc gcctcgatgc tcgctatggc ccggtccacg gcggcccagg
    16981 ttcgctcccg gtccgggaat ccgaggggca ggcactggat ggccagggcg gaacggatcg
    17041 tgctggtttc catgaacatc ctccatgtcg aactatgtct cggccgccca aaatgccggg
    17101 tcggcaatct agacgaccgg attcccttcg ggatcatccc aggtcgaagg tgtagagccc
    17161 cagggaaagg atcaaggccg tggtcaaggc ggcagccagg aatcccgccg cgcagtatag
    17221 ggtgtccccc cgtgtccagg ggctgtcctt caggaaggtc cgcggtccgg gcttgtccgc
    17281 gccccgggcg gccatggaca gggccacccg ctccccgtgc cgcacagccc cggccaggag
    17341 ggccgtggcc gtatgcgtcc ggtcgcggaa acggcgcctg cgggtcccgt accggagggc
    17401 ctgggccttt tcgatgatcc cgaggtcccg gcggaggagg ggtatggtgt tccacccggc
    17461 gaacagggcg aacccgaccc ggggtgcaag tccgagctgc accatgaggg cccggaccag
    17521 gtcgtagggg tcgacggcga agatgaatcc catcgacagg gagatgaggc tcacgctccg
    17581 caggaacacg gcggcgcccc gggccgggcc gttcaccccc tccgcggggg ccaggacgtt
    17641 caggacgaag agcccgacgg aggccaggga gaggacggat agggtcttga aatagtccgg
    17701 gtacctcacg ccccctagga tccggaggtt gatcaacccc agtaccagga aggcgagggg
    17761 ggtcgcgtag ccggtggaca ggagcaccgg cgccagaagg cagaccgtca cgaagagggc
    17821 ggccagggga ttacgcggat ttgagagcgc ccgcacgggg aacctcctcc cgaagtatcc
    17881 ggccgtcctt catgcggaag accgtatccg ccaccgcccc cacgaaaccc tcgtcgtggg
    17941 aaaccaggag caccccggcc cccgaatccg ccagggctcc caggtcacgg accagggact
    18001 cccgggcagc ctcatccagc ccgtaggtgg gctcgtccag gaggtacagg ggacgccgtt
    18061 ccgccagggc gacgcagagg ttcagcctgc gtttctcgcc ctcgctcaga cgataggggt
    18121 tggagtccag ccggttccgc aggccgtacc ggtccgcgga ttccagggcc tccgggccgg
    18181 acaggcccag ctccccccgg accgtctcgg ccacgaacat gtgctcgggg ttctggggga
    18241 tatagaggag cgatgcgtgg aagtcccggg gcttcagagc gagcgcgtcc cggcccgcga
    18301 gccggatgga gcccgggccg gcgggcagga tgcgggcgat ccgggcgagc acggtggtct
    18361 tgccgcatcc gctgggaccc gtcagggcgg ctacgcgtcc gggtcggacg tcaaggtcga
    18421 ctctccggaa gaccgggggg aacccctcga atccgtggga caggccccgg acctccaggg
    18481 ccggcggctc ggtctcggac gggaggctcc ccttcccggt ccgggaaccg gccgggggag
    18541 ggggggtccg gaaatcggag gggaccaggc ctccctccgg ggtgacttcc caggcccggg
    18601 cgcagacggc cgccgcggcc tcgatcttgt gctccacgac caccaccgcg gtccgggcgg
    18661 gcaggtcctc cagagcccgg aaaaaggcgg cggcggaggc ttcgtccagg taggccgtag
    18721 gttcgtccag gaagaggatg tccggttccc gggccagggc cgctgccagg gagacgcgct
    18781 ggcactcgcc tccggaaagg gtcaggggat ggcgtgccgc gaggcggccg atgcccagac
    18841 ggtccaggac ctccgccgtc cgcctgcgga tgaggcgggc atccaccccg tcgttttcca
    18901 gggagaaggc gatctcctcc tccaccgtgg gagtgatgac ctgggcttcc ggattctgga
    18961 gcaccacccc catcgaggcg tagaggtgct ctacgatgcc gtcgagggtc ccggggacgt
    19021 attccggggc caggcccgcc atcacctgca ggagggtcga tttcccggag cccgaggggc
    19081 cccggatcag gatccggtcc ccctcgcgga cggtaaggtc gatgtccttg aggacggggg
    19141 cccgcgctcc ccgatgccgg aagcccgcgg accgcaggcg aatcaggtca ggcaaggggg
    19201 ctgtcgtccc cggacaggag gttcgcggcc accccggtcc ggatgatggc ccggccggcc
    19261 cggtaggcgc cgaacccggc ccagaacgcc ccgcccgcca ggatgctcgc gatctggacg
    19321 ggccataccc agggctggag ggtccagtac tggctgtaga agaagtcgag gccgtaggag
    19381 aggatccccg cggccatccc cgccgcgatc atcacccgca gatcccagcg gcggtatccg
    19441 gtggccgcga acacgagctc gcagccgagg ccctggacga gaccccagat cgccgccgtg
    19501 aggccccact gggtgatgaa tccctccagg accgcggcga tcagctcccc gaagagggcg
    19561 gccccgggct ttcggatgag gaagggcacg agggtgcctc ccgtgaacca catccccgcc
    19621 aggaggtagc tcaaacccag ggacgaaagg ggggcggcca gggcggagac gaaggtccag
    19681 ccccagaaga gcaccccgat cgccacggac aggaccacca ggaccacgat ctcgttcagt
    19741 ttccaggatt tcttgacagc catgaagctc tccttgccgg tgcatccgga catgaggtag
    19801 gaatggctgt gggggagatg aatcggttcc cttcgccggt attatccgga tcaggttcaa
    19861 cgggtatgat ctcaggctcc caacgggaac caccccacga tgctataata gtaccgacgc
    19921 cggcggataa ggtcaatccg cggggaggga gtcgtagttg tccgttggct tcgttttttc
    19981 gggaacccgg gtccgacgga ccttatgcgt gtcggccgcc gcgctgaacc tggcgggatt
    20041 ggccgcgggt ttcctgtaca ccctggttcc cgccgtcgag gcggccggcc acttcttcgg
    20101 ctggatcctt ccgggcgccg cggctatggc ctgcgccgtc tgctcgatgc cccgggctcc
    20161 tcgccgcccc tcggccgtct acctggcctg gacgggcctg gggttcgcga tccttccctt
    20221 cctcctcata gcccgggcaa gtttcctgac cccattcgtc ttcgacgcgg catcccggct
    20281 cgtcgtcctg ggaagcctcg ggtcgggctt cgtggcggcc gtttcgcggc cggcctccgg
    20341 gaggccggac ttcgcgggga aggacaggag ggcgaggccc gcggcggccg cggccggatt
    20401 cttcgtggga atcgccgcct tcgcgattgc cgcccggttc ctgacgggac cgcacccccg
    20461 tacggccgga gttctgattc cgcaatgcct gccgtattgg tccctgagct tcctggccgt
    20521 cggggccgcg accgcacggt ccggcggagg cgaaagctcc acacgacgag gggccgggct
    20581 cctggccctg cccgcggtcg cggccgcgat cctggggggg atgccggttt accagactcc
    20641 cggagtactc cggaccgcgg agcgggacta ttcccgagcc ttcgagccgg agtccgcgac
    20701 gccgggtcgg ggaggctccg gtacggcctt tttcccgggc cggatgttcc tgggaacccc
    20761 ggccggccgg atccgcccga tcctggacgt gccctacctt gccgagacgg tcccggacgg
    20821 aaggatcttc accttccggt acgacgcctg gccgccgaat cataaggggc cgaggccggt
    20881 ccttatccgg atacacggag gtgcctgggt cagcggggac aagggctacg gcaacataaa
    20941 caacgtgaac cggcatttcg cgtcccgggg ctaccttgtg ttcgatcttc agtacggtct
    21001 cgcggaaacg ggaaccttcc gcccccggag ccccaccccg gagggtatgc tcggccccta
    21061 cggaatcgag gatatgctga ggcacatcgg tcgattcacg acgtacctgg cggaccgggc
    21121 cgaggagctg ggcgcggaca cctcccgggt cttcgtgagc ggagcctccg cgggaggcca
    21181 cctcgccctg gccgtcgccc tggccggcgc gaccgggtat gcccgggagc atcccggcag
    21241 cggtccggac ccccggatca cggtacgggg cgtgatcccc ttctaccccg ggatagggta
    21301 cgccgcgagc atggggattc cgacccctgc ggagctggat tccctggaac cgcttctcgg
    21361 gcccgcgaat cctcccgcgc tgttctatca aggttccctg gacggcatgg tcgatcccgt
    21421 ccgggtgcgc ggattcgtcg aggcctataa ccgcgcggga ggcggtcccg ccgtactggt
    21481 cgagtttccc tccgccggcc acggaagcga ccttatattc tggaacccct acggccagat
    21541 cttccttcga tacatggaga ggtttatgga tcgattatcc ggggagaatc cctgaggtag
    21601 gagggtttga tgaacggatc cacgaagcgc ggacggatcg cggcgctgat cctcttatgt
    21661 tccgtcctgt ccgccgcggc ggacgtgccg gttccccgaa tttctggcga cgaggtgctg
    21721 gccctggcgg cggagctctc gggccccctc tacgaaggcc gcgggatagg gaccgagggc
    21781 ggccgtcggg ccgccggcct gctggaaggg atgctccgga aggccgggta tcgggtccag
    21841 ttccaggaat tcccggagcg cccgggattc aaccgggggg aggccgggct ggatgtcctc
    21901 cttccgggtg gcgcccggca gaccttcgtc taccggaagg atttccgcga gaccgcccgg
    21961 ggagggtgga cgggggggac cgtggaaggc cccctgcgcc tcctggaagc ccgggggcag
    22021 gagttccccg gcggctccgt cctgctgatc ccggcctccc tctacgatcc cgccgccttg
    22081 gattcctacg ccgcccgggg agcgaagggg gtgatcctgg aactgccgga gggaagcccg
    22141 gcccagagat ccggatacac cggggcggcg cccggagccc tggtggagcc caaacagggc
    22201 atgatcgtgc tcgcggtatc ctcggatgcc taccggcgtc tggccgacgc cgcggcgggg
    22261 aaggcccggg tccgcatgtc cagtccggtc cgcttcgagg acgcccgggg ccgcaatatc
    22321 gtcgcggcct ggaacggaga cgggggtgac ttccggccca cgctggcctt catcgcccat
    22381 tatgaccaca tgggcaccga cgcggacggc agccgttttc ccggcgccct ggacaacgcg
    22441 tccggaactg ccctggctgt ccgtctggcg gaagcgatcg ccctcggctc gacccgggcg
    22501 gatttcgccc tggtcctcac ggacggagag gaagtgaacc tgggaggcgc ctgggccctg
    22561 gcccgggatc cgcccttccc gctgcgcggg gtatcggtcg tcaacctgga catggtgggg
    22621 tcccgcgcgg acatggagct gtccgtgttc cacaacggag atccggcgag ccggcagctc
    22681 gcggaccgga tcgcggaggt ggtccgggag gcgggcttca cggccgtcct ccgatcgccg
    22741 gtgtacaacc tggaccacgg gcacctgccc cgtgcggggg cccgggccgt gagcgtctgc
    22801 gagttcgaca cgagcttcta ccatacgaag gaggacctgc cggggatcct gtcggcgtcg
    22861 gaactttcgg tcctgggggg cgccttgctg aggttcgccc tggaggagct ggaatcccgt
    22921 tgaggcctcc cgattcccaa gatccgtttg tcctcgacag gagggcccgt atggcgtact
    22981 atgtccgcat accggaccgg agcccggcga ctccgccgcg cccggaggcc cggaaaaatc
    23041 cataggagca ggtatggcag agaaagagac cctcgttatc gcgtccaagg tcaaggcgta
    23101 catcaaggaa gccggggaca tgaaatgctc ggccacggtc atcgacgcct tgtcggacaa
    23161 ggtccgtgcg ctctgcgatg aggccgtcaa gaacgccaag gcggacaaga ggaagacggt
    23221 ccaggacaag gacgtccgct agacccttcc cgttcgcgtt ccgggaccgc gccgacggcg
    23281 cgttcccgga acgcgaagga tccgagcgga gagttccagc cgagcctgcc atgatagaaa
    23341 tcctcgccgt atccccgccg atcggggatc tggagatcgg tctccggtcg ttccaggagt
    23401 cctgcggaac ccgggacggc acgatccgcc tggagacgga gttcgcgctc aacgccttca
    23461 cggacatgcg ggccttgttc ctcgcgaggg attccgaccg cctggtcggg gccctctccg
    23521 tcttcgcccc cacgaggcac gaggcggaga tcaacgccgc ggtcctgccg gatcgtcggc
    23581 gcgggggcat cttccggtcg cttctctcgg aggcggaacg ggagctcgcc cgattcgggt
    23641 atgccgagga gctgttcgtg gtggattccg ggtccggccc cgggaaagag gcggccgccc
    23701 gcctgggcgg gacgcacgag tataccgagt ataccctgcg gtatgcgggt ccctgcccgg
    23761 aggtccccgc ccgggatctg gagatccgaa gggtgggatc ggaatccctg gaggatctcg
    23821 tggacctgcg atccggggcg ttcggcgatt cccgggagga gacggaggcc ttcgagcggg
    23881 cgaccctcgc ctcgccggag cggcaggtct acgcggcgtt cctgggggat cggatggcgg
    23941 gcgcctgttc cctgggattc caggggcggg aggtctccat aaacggcctc gtcacggccg
    24001 tggaccttcg cggccgcggg tacgcgcaaa gcctcctggc tcgcatcgtc cggaccctgg
    24061 aatccgaggg ctacggaatg accctcgacg tggaaagccg gaacgcggac gcgctccatg
    24121 tctatacgaa gctcggattc cttccggagc gggccgtgga atactggcgc aggtccctgc
    24181 ccgtttaggc cgggcgggat accttggagg cgtcgatctc ggaaaggacg gcagccccgg
    24241 tcatgagcat ggcctcctca agttccgcgc gcaccttccg caggtaggtc gataccccct
    24301 ccgctcctcc gccgtagacg gcgtaggcga agggccggcc gatcaggacg ccgtcggccc
    24361 ccaaagctag ggccttgaac acgtctagcc cggtgcggaa tcccccgtcg atgaggatcg
    24421 tggcctctcc tcccacggcc gccacgatct ccggaagcac ctcgatggtc gcgggtgtct
    24481 cgtccaggac tcttcccccg tgattggaga cgacgatgcc ccgggcaccg gcccggacgg
    24541 ccttccgcgc cccctcgggg gtcatgatcc ccttcaggat gaacggcacc cggaggctct
    24601 ccacgatccg ggaaatctct tccacgggca taggggccac gggctttccc atggcggcca
    24661 ggaccgtgag gcccgcggcg tcgatgtcca tggccagggc gaagactccc gcgtcctcgg
    24721 ccttcttggc acgctcgatc acctcccgga ctccccaggg tttgatcgtc ggaatgccga
    24781 tgccgccggc cgcgcggatc gcctcaaggg gctcgaagaa ggcgggatcc ttggggccgt
    24841 ctcccgtgaa ggccgcggac ccttccgcgg cacagccgct caccaccgcg gcggagtagg
    24901 tgccgtcggt gtacaggtcg ttgtagtgca ggcccaccgc cccgatggga gcggcgaaga
    24961 cggggaggcg gaaggtccgc ccgaagaggc gggtttccgt ggacacctcc cgggaccggt
    25021 aaatcgtgtc catggccggg cggatctttc gaagttcctg gtagttccgg atgaagccgg
    25081 ctcccgtgcc cttcccgccg ggtccgggta tctcaccccg gcaggccacg ccgttgcatt
    25141 ccgggcagac cctgcagtgg ggaccgatat tcttcttggc ggcttcacgg acttcctgga
    25201 aggtcatctt gcactccggc ggacaggatg cctgacttta tattgaattc ctgatccaat
    25261 caagggttat gggaacaccg caccgcgcct ccgcggagga acggactgcc gggacgcgaa
    25321 ggcgctcatc ccggtacgtt tgccagccgc cgggcgaagg gatatagtgg taccccgtat
    25381 ggagggagca tgtccgcata tttcgcagaa cccctggtcc ttcgttccct gaacatcccg
    25441 aaccgcttgt tcatggcccc catgtgccag tactcggccc gggacggatt gccgaacgaa
    25501 tggcaccttc ggcactacac cgagcgagcc ctgggagggg tcggcctggt cgtggtggag
    25561 gctaccgccg tttcccccga gggccgcatc agcccctcgg acctcgggct ctggaacgac
    25621 gagcaggtcc gggccttccg gcccctggtc aaggccgtcc gggattcggg ctgccggatc
    25681 gcggtccagc tggcccacgc ggggaggaag gcctccacgt accctccctg gcaggggaag
    25741 gggagggtgc ccgagtcggc aggcggctgg gaacccgtgg ctccctcggc cctggccttc
    25801 gacgaggact acgcccttcc ccgggaactg acccccgcgc aaatagagaa tctggctcgg
    25861 gctttcgggg aggcggcccg gcgggccctg gatgcgggct tcgacgcggt ggagatccac
    25921 ggggcccacg gctacctgat ccaccagttc ctgtccccct gtcgaacaag aggtccgacg
    25981 aatacggagg ggacctcgcc gggcgctcgc ggttcctggg gcaggtcgtc cgcgaggccc
    26041 ggaaggccgt tccggacacc gctccggtgc tcatccgggt ttccgccacg gactggatcc
    26101 ccgggggctg ggacccggag gagtgcgccc gggtcctgaa cgagatcaag gatctggggg
    26161 tggatttcgt ggacgtttcc tccggcgggc tggccccggg cgcgaaaccc ccggtgggtc
    26221 ccgggtacca ggtgggtctg gccgcccgga tccgggagaa gaccggcctg cccacgggag
    26281 ccgtcggact catcaccacc gcggagcagg cggaccagat cctgctctcc gggagcgccg
    26341 acgcggtgtc cctggggaga cttctgctga gggacccctg gtggcccgcg cggaacctgc
    26401 ccgcggacag gcgccgcatc cctccccagt atctccgggc gtactgaccg gacccgggcc
    26461 cgccgtatcc gggccggcgg gcccaggcgg cagcggggtc atacccctcg gcgtcggtcg
    26521 ccccgggccg ctctcctagg ccggcactcc cgcggatacc cagagctccg cgttcagcac
    26581 ggcggcaccc gcggcgcccc gttccaggtt gtggcccagg gccaggaact tcaggcccag
    26641 gatcccgcag gaacggaagc ggcccacgga tacggacatg ccgtccccgg catccgcgtc
    26701 caaaaggggc tgggggcggt cggcctcttc ccggaccgtc acgggatact cgggggcgct
    26761 ggacaggccc aggtccgcca ggggggagcg gtaggccttg aggcagccca cgacctcctc
    26821 gatagacgga ttcccggcca gccggacgga ggcggacacg ctgtgcccgt cccgcacggg
    26881 gacccgggta caggtggccg aaaccgggaa gcccgcgggc tccacccggc cgccctgcag
    26941 ggtgcccagg atcttggccg tctcggtctc cagcttttcc tcctcccccc ggatgaaggg
    27001 gatcaggttg cccgagatgt ccagggcgga gaccccgggg tacccggctc cgctgaccgc
    27061 ctgcatgctg gtgaccaggg cggcctcgat cccgaaggtc ctgtgcaggg gggccagagc
    27121 ggtggtcagg atcgcggtgg tgcagttggg gttgcaggct atgccgcccc gccagcgcct
    27181 gcgccggcgc tgttcctcca gcagggccag gtgatcggag ttcgcctccg gcacgaggag
    27241 gggcacgtcc tcgtgcatcc ggtacgagga cgcgttcgag aagaccaggg cccccgaagc
    27301 cgcgtacagg ggctcgagct ccgaggcggc ctgggcgtcc agggcgctga agacgacggg
    27361 cgcccaggcc cgctccgggg acgtctcctc cacgatacgg tccgcgagcc ccccgtaggg
    27421 gcggcccggc gctctccagg tgcaggcttc cccgtacttc aatccggcgc tccggccgga
    27481 cgccgaaagg tatttgatct cgaagagggg atgctccgcg agccgtgcca cgaatctctg
    27541 tcctacgatg ccggtcgcgc ctagaatcgc gaccccgatg cgttccatgg gtatacctcc
    27601 gtcctttgag tgcgatgagt cgtttgcgtc gtgatgcgga tgatcagagg ttggaacgag
    27661 tcaaccagcg gccttggtat gcgcgaagca agagcgatac gggattcgcc actcgccccg
    27721 cggatcgaag gaattgcatg gatcctccaa tccgaaggag agcccgggtc gggcggttcc
    27781 ctgcctcgag aggacacgga atcgccccgc ctttggcact ccgcgggccc ttgggatttg
    27841 cccgcgacag ccgtcgggtg ttagcccgaa cgtccaaggg ccggccatgc ggatgcccgt
    27901 ccccttcggc ggtcctcccc gttcacgccg gcgtctacgg gcaccgcgcc ctattccggg
    27961 ttactgatgg ggaccgctcc tccatcggta cttcggaacc tagcacggag ggggaaaccc
    28021 cttcaaggcg aagccggatg aatgccgtgt tttccgcgta ttttcacgta gaaacgggcg
    28081 gaatcagacc acggcgtcgc tactcaaatg atgatgatct cacccgaaca acgtgggaag
    28141 cacatagtcc tccaactccc ggcgaacccg cggcagatcg atggtcgtga acgccccgtc
    28201 ccggtagagg atccggcccc ggaccatggt gaggacgacg tcgctgcccc gggcggagta
    28261 gaccaagttg gacaccacgt tgtggcaggg gaccaggtgg gggcggtcga agtccaggag
    28321 gatgaggtcc gcgtccatcc cggggaccat gcggccgcac tcgtccgccc ggccctgggc
    28381 ccgggccccg ttgaacgtgg ccaggcgcag ggcgtccaag gcggacacgg cccggggatc
    28441 cccgcttgcc cccttgtgga tcagggcggc ggtcttgatc tcctcgaaga ggtcgtggct
    28501 gttgttggag gccacgctgt ccgtgccgag ggctacgttg atccccctgg ccaggagggc
    28561 gggcaccggg gcgatgccgc tggcgagctt caggttgctc acgggattgt gggcgacatt
    28621 cactccccgg gcggccagaa ggtccatgtc atccccggtg acccggacgc agtgggccgc
    28681 cacggcccgg gtcccccaga ccccgtaccg gtccaggatc gcagcgggac tcgctccgta
    28741 cttggcgatg cagtcctcgt gctccttccg ggtctccgac aggtggacgt gcatccccag
    28801 gccgttctcg gcggcgtact ccgccacggc ggcccagagc ttcgggttgg acgtgtactc
    28861 cgcgtggatg ctggcctcga tccggatacg tcctccgtcc cggccgtccc actcccggtg
    28921 gaggcgtcgc atctccacgc agcccgggtg ggactcgaag gaaaaatcct cggagaaaag
    28981 ggaggtgccc cgggagacgt tggccttgat gccggattcc tccaccgcgg ccaaaatgtc
    29041 gtcgcagaag taatacatgt ccgtgaagga cgtggtgccc gaggccaagg cctcggctat
    29101 gcccagcagg gcgccggccc ggacgcagcg gccgtccagc ttggcctcca cgggaaagat
    29161 gtacttcgtc agccaggtct gaaggtcgta atcgtccgcg taaccccgga ggaccgtcat
    29221 ggggatgtgg gtatggcagt tgacgaggcc cggcaggagg accttcccgc ggccgtcgat
    29281 tcgttccccg gaaaagtccg ggggaggcga gtcggtcacg gatgcgatcc tgccgccgga
    29341 gacgagcacg taggagtccg gccggaccgg cgtgtcgccg tccatcgtga ccgcggtcac
    29401 gttctcgaat agggtatccc tcatggctcc tccctcagac tcgcgcagcc gccgggacct
    29461 cgcaacaggt cttggtcatc ggtcgcttga aggtgtccac gcaggcgcgc aggatcgcgg
    29521 cccggttccg ggacatggcc tcctgcacct gtcggagggt agcctcctcc tcggacaggc
    29581 ccgtgcacat gttcgtcacg atccccacgg aggcgtagca gatccccgcc tcccgggcca
    29641 ggacgacctc cggcacgctg gtcatcccga ccacctcgcc gcccatccgg gcgtacatgc
    29701 ggatctccgc ggcggtctcg aagcggggcc cctcggtgca cacgtagacc gcggaaccct
    29761 tgatccaaag acggtggtcc cgggcggtct cctcgaaggc ctccgtcagc ttccggcagt
    29821 agggttcccc catgggcacg tgtcggacct ccgtgccgtc ctcgaagaag gtggagggcc
    29881 tgcctttcgt gaagtccagg aagtcccgga ggacgaccac gtccccgggg gcgaatcgtt
    29941 cggaacagga gcccacggcg gcggtcgcgt agatgaaccg gattcccagg tccttcaagg
    30001 cctggatgtt cgctcgatag ttgatcctgt gcggagggac cgagtggccc cgaccgtgcc
    30061 ggggcaggaa ggcgaaccgg aatccgtcga tcgtcacgag gtccacgtcc gcggagccgt
    30121 acctcgtatc caccgtgacg ctctccttgt cgtcgaagag cttgtagacg ccggtacctc
    30181 cgatgatcgc ctgattcatg gatttccttc cttccggccc ttttaaaccg ggatgcccgg
    30241 cattagtata tcggatacat gaagatcatc gtagggcatt ccaacatgga cttggactgc
    30301 ataggctccc tggtgctggc gcagtacctc ttcccggacc acgttcccct gcgaagccac
    30361 ctcatgcagc ccgcggcgcg caacctgatg aacctgtacg ccgaccgtct cgattttccg
    30421 gggatcaagg acatcaaggg cgagaaggtg gagcgcgtcg tcgtggtgga cgcccggtcc
    30481 gaggacagga tagccgaggt ccttcccctg atcgagccgg gga
//
LOCUS       DAOTEI010000021        29620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1036_length_29620_cov_5.045290, whole genome shotgun sequence.
ACCESSION   DAOTEI010000021 DAOTEI010000000
VERSION     DAOTEI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 29620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29620
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1036_length_29620_cov_5.045290"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="P5313_03440"
     CDS             <1..236
                     /locus_tag="P5313_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY79451.1"
                     /translation="EIAAAMLQRQQATAIIAARQMIVEGAVGMVEMALEKLSDKNIVN
                     LDEERKAAMVSNLLVVLCGNKDAQPVVNSGSIY"
     gene            256..420
                     /locus_tag="P5313_03445"
     CDS             256..420
                     /locus_tag="P5313_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRY79452.1"
                     /translation="MDGTDKEGGKKQILLRLSPSLWRELAAWAEDDFRSINGQIEYLL
                     TECVRQRKKK"
     gene            526..1734
                     /locus_tag="P5313_03450"
     CDS             526..1734
                     /locus_tag="P5313_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRY79453.1"
                     /translation="MALKHIGYRVIQNVLRLTSYFMSFRPPELLEGPGSVKRLPALIK
                     EKGFSKVLVVTDKGLRAAGLPDGLLAGLDETGIGRVLYDGVQPNPTIGNIEEALSLFV
                     REGCQAVVAFGGGSPMDCAKACAARAANPRRTVRRMRGLLRAGRKPKPLFAVPTTAGT
                     GSEGTVAAVIADPETHEKYSISDPKIMPRWAVLDPELTLGLPPNITSTTGLDALTHAV
                     EAYVGRSNTAGTRAMAEEAVRLVFANLQAAYKDGRNLAARTAMLRASFCAGSAFTRAY
                     VGYVHAIAHQLGGLYGIPHGLANAVILPYVLDFYGNAVAAPLARLAEEAGVGSAGMDE
                     ESRARAFTAAVRDLSRRLSIPERMDGVREEDIPLIVRRALAEAHPLYPVPKFMDAADC
                     EAVVRRVAGK"
     gene            complement(1921..2493)
                     /locus_tag="P5313_03455"
     CDS             complement(1921..2493)
                     /locus_tag="P5313_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRY79454.1"
                     /translation="MVKSILLVGVGGQGTILAAKLLTTGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDEKVHSPVIEVGGADILVSFERMEALRWLRFLKPGGRAIVNDYRINP
                     MPVVSGRAEYPEGILEELKSKADASVVDAAAKASELGNPKVMNVILLGAAIRSMGLGS
                     IDWARIIRENVKPAFVELNLKALEVGLGLV"
     gene            complement(2504..4285)
                     /gene="iorA"
                     /locus_tag="P5313_03460"
     CDS             complement(2504..4285)
                     /gene="iorA"
                     /locus_tag="P5313_03460"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRY79455.1"
                     /translation="MKKLMTGNEAVARGAWEAGVKYASAYPGTPSTEILETIAEYRGD
                     IKAEWGVNEKVAFEAAMGAAIAGARSLAAMKHVGLNVAADPLFSMAYTGTNGGFVVIT
                     ADEPGQHSSQNEQDNRNYAPFAKVPLFEPGDSQECRDMVKEAFRVSEDYDTPVLVRMT
                     TRTSHSKGIVECGDREEAAIREYRKDAVKYVSVPAHARARRLVVEDRMRRLADYSEAS
                     PLNYVEDNGSEVGVIASGTAFFFAKEVFGSTASYLKLGFTNPMPVRRIRDFCGSVKKI
                     YVIEENDPIIETQVRLLGFDCLGKKVFPFCGELTSDIIRKSVFGSCFDALEADPAKIV
                     ARPPTLCAGCPHRGFFYELGRRRNVMVSGDIGCYTLGFAEPYNAMDTCVCMGASISAG
                     HGAQQVFDMRKDEKPRRVVSVLGDSTFFHTGINSLLMVGYNRSRTVNVILDNRITGMT
                     GHQQNPGSGYALDGSRTPDLDFEALVRACGISNVRTIDPNDLKLVKETLDWALALDEP
                     SVIITRWPCALKRFSPEDKKEFAGAFQSRYRVDRDLCIGCKACLRSGCPALAFDFAGK
                     KSGIDPDQCVGCGVCAQICPKSAIVRE"
     gene            complement(4320..5237)
                     /locus_tag="P5313_03465"
     CDS             complement(4320..5237)
                     /locus_tag="P5313_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HRY79456.1"
                     /translation="MAQESFGNFTDILAICRKLGKVRISVAVAQDEDVLRAVWAARDA
                     GIADAVLVGDRGRIEAIAAKAEVGSLDIVHEPDEVAAARRAASLVREGRADALMKGLV
                     NTSDFLRAVLDPDSGLRKGSLLSHLAAFEVPGLSKLMFHTDGGINIAPGLDEKKAILL
                     NALGALARVGIREPKTALLSANEQVNPKMPSTTDARALVEMRARGEIPAGILEGPIAL
                     DVAVSRRAAEHKKIRSEISGDADLFLVPNIEAGNLLGKALVYYAKAKMAGLVLGAACP
                     IVLTSRSETAEGKLHSIALASLIALSPAP"
     gene            5367..5825
                     /locus_tag="P5313_03470"
     CDS             5367..5825
                     /locus_tag="P5313_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRY79457.1"
                     /translation="MDALDVRLLDLLQQDARMKISELSKRLNLSRPSVSERMARLQDA
                     GIIEGYSARISLKAIGRDTMLLIRLSSLKVSPRIFEAKIEADQDILECHRVTGEVDYL
                     IKAAVSNMDEMRHLIDRLMPFGDVSTSIVILSPIAHRRILPRAAEGRPVE"
     gene            complement(5868..6413)
                     /locus_tag="P5313_03475"
     CDS             complement(5868..6413)
                     /locus_tag="P5313_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HRY79458.1"
                     /translation="MNRTRILKLAVLAVAVLAAAAPLAAQGVAPSPKTPAADGTVSPG
                     EYSYTSAQKDMTVHLSLSPDGRTLYAALEAPTTGWVSVGLGSLRMDGAFMVLGYDAAG
                     TAAVSEETGSGRRHTPNPARILTAQAVRETAGKTVLEFALPAAPHAAGSSLKLILAYG
                     RRDDFTSIHARYAPVEVPISR"
     gene            complement(6410..7075)
                     /locus_tag="P5313_03480"
     CDS             complement(6410..7075)
                     /locus_tag="P5313_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79459.1"
                     /translation="MKKVLFYLGYLVSFAVPLYFYYQSVGAYLDGYTASVLLGVSAFV
                     LLCDQFILASKPGFAVKALGARGLLALHRTAPAALVLIVAAHFTLKKAAGFGTTSLRA
                     LLGIGAFTILLVLAAFAAVIMANTVLSRIGFLAAFKKHFTEHTGMTYKKARDLHNAFV
                     LVAYLVLIHALLASTGDFGMNPIGVSWLIFWFVLSQGMYARYRLTGRGGAEKKAPKEG
                     GTK"
     gene            7268..7738
                     /gene="bfr"
                     /locus_tag="P5313_03485"
     CDS             7268..7738
                     /gene="bfr"
                     /locus_tag="P5313_03485"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRY79460.1"
                     /translation="MKGNEKLLSALNGLLADELTAINQYMVHSEMCDDWGYEKLHKHF
                     EKRAIDEMKHAEKLIGRILFLEGIPIVSSLNKITIGATVPKQLDNDHAAETGAIKAYN
                     EAIILAGEVKDYATRDVLQSILNDEDAHMDGIEALQSKIEQMTLQVFLTTQVGE"
     gene            7735..>8394
                     /locus_tag="P5313_03490"
     CDS             7735..>8394
                     /locus_tag="P5313_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HRY79461.1"
                     /translation="MSEYLSRGPGAEDRAGRLKVIIEKLAAGAPPSAVKKEFHDLIKD
                     ADASEVASLEQSLIEGGMPVEEVQRLCEVHADVFKAGLERGERADRMPGHPVHTYRAE
                     NVEARKRARALSLAAFAGGIPAVKAAMDGLRPIIVHYERKENQLFPYLERTGFTGPSK
                     VMWGKHDEIRAAFRDLDAALGAGDVRSARAKARDLVRRVRMMIFMEERILLPNALKRL
                     TD"
     assembly_gap    8395..8404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8405..9010
                     /locus_tag="P5313_03495"
     CDS             <8405..9010
                     /locus_tag="P5313_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY79462.1"
                     /translation="DWAEVRRGEDAIGFAWVKPGARWDPALAVQAAGGGAYSNFDAAL
                     RTAAEAAAAKAGSETSEPEKPGGGAIALSTGSLPLDVLDRILKSLPVDVSFVDADDKV
                     MYYSDSPHRVFPRSPGIIGRSVQNCHPHKSVAVVEKILESFKRKEKDKADFWIEMGGR
                     FIVISYKPIYDDAGTYLGTLEMSWDATEVRGLTGQRRLLDW"
     gene            9505..10698
                     /locus_tag="P5313_03500"
     CDS             9505..10698
                     /locus_tag="P5313_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HRY79463.1"
                     /translation="MKKQMTATVSWVGKVDWELTKFHGDEYSTHKGSTYNSYVIEEGK
                     TVLVDTVWKPFAKEFVDNLEAEGLLGKLDAVIAQHAEIDHSGALPELLRRRPDLPVYC
                     TANGIKSLKGHYHQDWDFHPVKTGDEFDLGNGKKLVFVEAPMLHWPDSMFSYMTGDAI
                     LFSNDAFGQHLATERLYNDLVDQDELYDECVKYYANILTPFSALVKAKIEQVLSLKLP
                     VNMIATSHGVIWRKDPTQIVLKYMEWCQDYKEDRITILYDTMWEGTRALADEMARAIQ
                     EASPSTTVVSHCLSKFDKNDALTDVFRSKAILVGSPTIGKGILTAVAGTLEEIKGLRF
                     KGKKAAAFGCYGWSGESVKIIGDALRESGFEVVDEGIRALWNPDTDALKAARDYGKAF
                     AAKVK"
     gene            10938..11372
                     /locus_tag="P5313_03505"
     CDS             10938..11372
                     /locus_tag="P5313_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY79464.1"
                     /translation="MVVEEPVEDRFVEALCVLRARIFEGSPAESSGLTPALAKALLAI
                     TDGGERHLRVRELAESLGIKESSASVLADRLVQAGLANKKPDPVDARALLVGASPSGS
                     KLAEAVRLHRRRIARSLMEVLSREESQRLLEYLEKMGKREEY"
     gene            11399..11851
                     /locus_tag="P5313_03510"
     CDS             11399..11851
                     /locus_tag="P5313_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRY79465.1"
                     /translation="MAVAALIWALVGDREKARAGLRKGLQVMMGIAPVTLLVVAVVGI
                     VLAFIPAETVGLVLGEGAGFTGVLAALGLGALVHMPALLGFPLAGKVLQNGGSPGPVA
                     AFITSLTMIGVFTIPMEIKILGRRFTLARNLVSAALALSIAVLMGVLV"
     gene            12016..12441
                     /locus_tag="P5313_03515"
     CDS             12016..12441
                     /locus_tag="P5313_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79466.1"
                     /translation="MFILPAVCVLMGLFGAFVSKDLVMRILGKSSGWKGALLSIGLGS
                     LPTGPLYAAFPLAAALREKGARSANIFLFLTAWACIKLPQELLELRFLGPRFMLIRLA
                     LTVALAVPAAFLVERYVDSVASRSEPESPATSNGKEMRP"
     gene            12438..12773
                     /locus_tag="P5313_03520"
     CDS             12438..12773
                     /locus_tag="P5313_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY79467.1"
                     /translation="MIESAFPFARTDSQTMEKIVDDERVNINHIVVEPGKAVPTHVSN
                     SFVHQVVVRGTLSLSLEDGPATPHPAGTIVAVPFNTKMAIQNEGTETLEFFVVKAPNP
                     RDMPPVKAI"
     gene            complement(12974..13873)
                     /locus_tag="P5313_03525"
     CDS             complement(12974..13873)
                     /locus_tag="P5313_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY79468.1"
                     /translation="MSASLRSRFWKAAIRAAFRDKGLSIPELRARSERNGRFHPPFPT
                     DTGIEEIVLEGLRCAAIRPPRERPEKVVLYLHGGGYVTGSIESYWMLCVPLARALGLR
                     VVLPEYRLAPEHPYPAALEDALKAFRGLLAEGHAASDIVLAGDSAGGGLCLAAALSLR
                     DSGSPAPAAVICLSPWADLSNSGASHAANAKSEALLDTGTLDRWAALYSGGARLDDPY
                     ISPVHGDFRGLPPLLIQVDRGEILLDDSRTVAEKAEAAGVDLSLSVWDGLWHVWPALG
                     DLVPESGAAFEEMREFLKARSFL"
     gene            14157..14858
                     /locus_tag="P5313_03530"
     CDS             14157..14858
                     /locus_tag="P5313_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79469.1"
                     /translation="MAILQLSDVHKTYPLGKTEVHAVRGVSLEIQKGDFVSIAGPSGS
                     GKSTILNLIGCIDRPSGGSVRLGGVDTAKLSDRELTRLRHRVLGFIFQSFNLIPVLDV
                     YENVEFPLLLGREVPKPAERRERVGRLIREVGLETWTRHKANELSGGQRQRVAIARAL
                     VTRPEVVLADEPTANLDSATGESILALMQAMNREFGTTFVFSTHDARIVDLADHVVRL
                     KDGRIAEEFRRGGAA"
     gene            14855..16318
                     /locus_tag="P5313_03535"
     CDS             14855..16318
                     /locus_tag="P5313_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79470.1"
                     /translation="MILLRLAFRNMREHRAKTLIVGVLVTLGAFTLTAGNSFLDSLRA
                     GQRRTFVESFTGDLIVRAESDEAYSLTVSPARSPAPELPGFPEVRERAEALDGVAAVL
                     PLVNSMATLSAGETAAGFSMLWGVDFAEYRAMFPESLELVEGDWPAEPGAWILVSEAT
                     RREAEAEAEVPVRVGDRLLVTGMGEGGTKIREVTLAGVFRFRRGNNLLDRISLTDPGT
                     VRSLAGLTLARREAASSESSETGADAAVAAPAVTDEDALFSGFSIAAAETSAGPADFD
                     SILGDTSARARYSAVDSDAWNFLLIRLADGRDAGRAAEELGAAIGAGTSGLVVSDWSW
                     GAGVQADLSRYLRLAFNGIVLIVVAVAAIIIMNTLVISITERIPEIGTMRAIGAGRAF
                     VRRMILTETLMVSLAFGALGVLAGCVAVGILGRAGISSSNEILATLFGGEVFRPAVSA
                     AAVAGALAAMAMVGVLSCLYPVSVALRITPVRALQKA"
     gene            16331..17662
                     /locus_tag="P5313_03540"
     CDS             16331..17662
                     /locus_tag="P5313_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY79471.1"
                     /translation="MNQSMRIAFRNVGRQKKRTVLLAGAVGLGFFIITLSGSLLGGIA
                     ASARKNFSAAFGGHLYFSGSVVSDRGSEIAVMADPGPLESVLAAHARDIASIHRRSGT
                     SGTLIFGTRELRQTLTGVDFGREPDFAGGLRIVSGSLDGLSEPGALVLPEDAAERLGV
                     RTGEAVLFRASTVHGQRNVTELVLIATYEGQSSFGVSAGYAGLGTVNSLLGLGPEEFQ
                     TLNVWLRDDRAIDAAAGRILADLAGKVPVEPREDEDPMKRMAERLLGGSGPRSVGDGE
                     GWEGTRYGLTTLNDLMAQFTSILSIADALSFWVFLILLSITMVGILNSYRMVMVERTA
                     EIGTLRALGLQKGGVRDIFLWEALFVSLYGALAGLAAALAVMALSRAAVLPGGTVLSF
                     FLDSGRLPFSVDPIETVRNLVLLCAASLAAVSFPARAAARLPPAAALRAAY"
     gene            17741..18511
                     /locus_tag="P5313_03545"
     CDS             17741..18511
                     /locus_tag="P5313_03545"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRY79472.1"
                     /translation="MRLRTFAAAALVLASFLRAAPLAAQASGTPDFKALLAAVDGRTN
                     FRASDFSARLTLVSEDPSKGTETRSVRVFRRDRADTLLLLVLKPESELGQGYLRVDDS
                     LWFYDPESRKFTHSSVKENFQGTDAKNSDFGLSSLAEDYAVAAHGEGRLGAHETWILD
                     LKARHDETAYASRKVWITKKDGLLLKSEDYSLSGRLLRTAYYTSYARVGSSLICDRFT
                     YVDALVAGKKTSVLMEEISLRPVPDEVFTKAYVERVNR"
     gene            18522..19874
                     /locus_tag="P5313_03550"
     CDS             18522..19874
                     /locus_tag="P5313_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79473.1"
                     /translation="MDTTRFGRALAALLLALAVRTASAQAAPSPEQEIPAAPSPADEE
                     FFGAGDDLVTEAPEAGDADSGGAGLPEFQKVRWGGTFRLEAAGTADAEALARGEWAGA
                     PGAELDLSGTFFLDARPSEEVRVLAKARAAYPFEDAGDWALRELFADLALQPGLRLRA
                     GKQTVNWGVGRFWSPANVINLERIDPEHPDEELAGPVAARLHAVRGTSNLYAYALLQG
                     MEEDGTVGIAAKAEGLVGGTELSLAGVWRPDRPWAVAATATGPALGLDLYAEAVVRGG
                     GRRTYVVEDGGAPLGVSAESREAEPAFQGTLGFSWTWSDETEAWNLAASAQYLRNGAG
                     YADPDLFTTYAAGVAALAAAGQIGPEDLYLRGRNYGAASLALSDIARSDAGLSLLWLG
                     SLDDRSGKVVASASYSGFKNLRIALSYGERYGRAGSEYGFPGPRRTLGLSVETTGLSF
                     "
     gene            19889..20596
                     /locus_tag="P5313_03555"
     CDS             19889..20596
                     /locus_tag="P5313_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY79474.1"
                     /translation="MDARILVIEDEPDLARLAGMYLEREGMTCALAPSAEDALVRLAS
                     ERFDLVILDINLPGMDGFEFLRRLRRDDRVPVVVVSAREADEDVVLGLGLGADEFIVK
                     PVAPRVLAARVQALLRRLREGSTARRLLRFGDFELDPEACVLTRGGRRVGLSGKEVDI
                     LVFLASAGGRPSTPKEIYEKVWGQVYGDLSIVGVYIQRLRRKVEEDPAAPRFLETVYG
                     KGYRFNPGELKRAGPWD"
     gene            20587..21891
                     /locus_tag="P5313_03560"
     CDS             20587..21891
                     /locus_tag="P5313_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY79475.1"
                     /translation="MGLKARLLLLMTGIAVLPVLSSALLILYLRTQYPGFAALDSSPL
                     ELGIRQMETRLAEALEEGDLERFLRGSENLSIRVEESDTGRIVFERAVPAGGPGADEL
                     AIRFSAGGRGYRAVFRSDSPPDELGPSLILPLLPVAVLGIVILFTVAVSFGILRSLGR
                     SMGKLEEATRRIAAGDLDFEPALPAGDSLASLAASMDAMRLRLKEEYQRRDRFILAVS
                     HDLKTPLAVLEGYLDAFADGLADSPEKRESYLKVLREKTGVLAHRISHLVELARMTTV
                     EWRQTFEDCDLSAFLEETLGLLADEAAARGRRMEVDFRLSPGLRAALNRDMARRVLEN
                     LADNAETYSPPDSVLRASAREEGAEVLVRISNEGEGIPAERRGLVFEPFYRGNPGRNA
                     GGFGLGLASVKSIAEAHGWGIDLESVPGGTTTFTVRIPAPRS"
     gene            complement(21888..22517)
                     /locus_tag="P5313_03565"
     CDS             complement(21888..22517)
                     /locus_tag="P5313_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79476.1"
                     /translation="MSETETRKDTYHHGHLREELIRRGLDALEQEGAEALSLRALSGS
                     AGVSKTAPYRHFKDKEEFLGALADEGYRLLHEALAASIAPGRKRRRAETPVGAMGRAY
                     MAFAVSRPALYRLMNSPLMCRLPQEYTGWARKSLMLLAGTLEAARTGAEGSPDVRQAG
                     PPDLDATAAAWAYIHGLVLIRIDGLYPTDLPEPDWDRLAGVVPELPTGS"
     gene            22694..23398
                     /locus_tag="P5313_03570"
     CDS             22694..23398
                     /locus_tag="P5313_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79477.1"
                     /translation="MVDSNMRKTGGIAVFVLAGLFTAAFAGEPAALPAPKPGTVSGQR
                     LELRLEPGPHYAKDMRMLWIQYTVWPQVAAWIETPDGRFLDTLYVTELAVTGKYRAAP
                     KSGRPEALPVWSARKSGAADAVSSPTTVGSPVVYGNGIASRLPAGRYVVMLETNRSYD
                     WNGTYTKKNSGVNGQPSVVYRAELEIGSGPREARLTPIGIGSVDGSDGKVRPGLDGID
                     TALELFSAMTVKYLDK"
     gene            complement(23452..24018)
                     /locus_tag="P5313_03575"
     CDS             complement(23452..24018)
                     /locus_tag="P5313_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRY79478.1"
                     /translation="MTDRFDIYIAGVGGQGIGLLSEALIRAADAAGLTARGCDTHGLA
                     QRGGSVASHVRIGSRCHSALVSEGSADLVVALEIHEAVSAGRAYLKEGGTLVWCDASW
                     QPLAVRLGTERPAEVSDVEEVAALRRGRAVRVPADAVPDPRMQNTAVLAAICREGLVP
                     GLGLEHARTALTDLMSGPALEANLRLLA"
     gene            complement(24015..25907)
                     /locus_tag="P5313_03580"
     CDS             complement(24015..25907)
                     /locus_tag="P5313_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY79479.1"
                     /translation="MKDLGETLLRKEPFSEIVIGNTAIVRALVESGLRSVTAYPGSPT
                     PEIADAIAAIPADRRPFRFEFSVNEKVALEVAFGSALAGRPSAVFFKSVGLNVAADSF
                     VQLPLLDIPGGLVVILGDDPGANSSQNEQDNRHYARMSYMPLLEPASAQEAYDMFKTA
                     ARLAARHRTAVILRLTTHVCHAKEKVDFEALPPAFPRPAWAFDPERENYIPLAATVFP
                     LKRRALERLAAIGRDPEALGTNFVEGSGSRRGVITAGTAWLSLRDALEETKPGSERPD
                     VLKLGLVHPLPRDAVLAFLRAREEVKVLEELDPVLETEIKALAYDEGLSTRILGKRGV
                     EDLMGECVPAKVRDILSGTWPDLFDAPRPAPLPASAAPRPPQMCPGCGHRSAFYAIKR
                     ALSPGDVTVADIGCHTLGYLPPYRMGGVLLSMGHSTSTASGLSIFREGRRVVCFLGDS
                     TFFHAGMPGIVNAVYNGHEVLLVVMENGTTAMTGHQDHPGTGRSFNGPAPKLSVRAAL
                     EGLGVKNIREVDAYRQAELTALVREALAEPGFKVIIARHPCMLKLTREARRRAGAAWK
                     VRQVRVDPDTCVKNHECIERFACPSFQAREGGAVEVSRDLCIGDGSCRQTCPAGALVQ
                     DKEEGR"
     gene            26091..27413
                     /locus_tag="P5313_03585"
     CDS             26091..27413
                     /locus_tag="P5313_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY79480.1"
                     /translation="MAKSTKRKCKFDPERPYCLVLGGGGAKGVYHIGAWRALRELGVP
                     VNAYVGNSIGAIIGAFLAQGLEKELEELGATIRLESIVDVGPEKDGLPDGSEMVSRIK
                     KVSRSLARKGGLDTSPLRSTLMELIDEKVIRSRGCDLGVTTVNVSDLEPEEIFLDSMP
                     PGSLVDYLMASSAVPGFTLPKIQGKTYVDGGVYDNVPYEMARRRGYRRIIVVDISGAG
                     INLKPDVEGSETVYIKNSIQMGGILDFDRAFLDRFTLLGYLDTMRVFGRLSGYSYFLR
                     PDPEAEARFRGEAARLGPPLAGEAERPSKDFFPDRMRLDGRLLLKCLECSAQSLHVER
                     IREYTYAELASEVARKRAEEEERAKHAIGSGEARGARRLEQVLAAAWKDGRFEKPPYF
                     YLLLARATPAGKAREAVHMLLDRFFPELKAAEAWFGVDPRILEEVISR"
     gene            complement(27543..28043)
                     /locus_tag="P5313_03590"
     CDS             complement(27543..28043)
                     /locus_tag="P5313_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY79481.1"
                     /translation="MVGTEIRELHFIEDIGLFFEQMGMPRMAGRILGVLLICDPPEQS
                     LNDIGEAARASKASVSTMTRLLASSGLIEQVPSPVPRRDYFRYRKGGWLSFMRQRMEI
                     MAALHDITEKGLGILDGKDPALRERLCEAHDIFEFVENEFPDWLERMAAKRGANLGRR
                     RLGDRP"
     gene            28214..>29620
                     /locus_tag="P5313_03595"
     CDS             28214..>29620
                     /locus_tag="P5313_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRY79482.1"
                     /translation="MPDSFDPADLRGLSSEEVLERLSSEGYNELPQGKKRGLFHIVFE
                     VFREPMFLLLVACGLLYLILGDPEEALMLLGFVAVIIGITVYQEGKTEKALDALRDLS
                     SPRALVVRDGEQRRIAGKEVVRDDIVLLAEGDRVPADALVLHQVNLSADESLLTGESV
                     PVRKAAWDGKADPGRPGGDDQPMVYSGSMVVRGQAVVRVTSTGIQTEMGKIGKALQTI
                     ETGQTRLQAETGRIVKSFALAGLFLCVLVVLVFGFTRGNWLRGVLAGLSLAMATLPEE
                     FPVVLTVFLALGAWRISKRSVLTRRMPAVETLGSATVLCTDKTGTLTMNRMTVAELCV
                     GTRVSKVGQADNLLPEEYHALVEHTLLASPKDPFDPMEKAMRELGVRTLMNTEHLRDD
                     WKLLREYPLSEKLLAMSRVWESTVDGGFVVAAKGAPEAVSDLCHLDAAAASEVRACVD
                     RMAADGLRVIAVASSRIPA"
BASE COUNT         4805 a         9886 c        10129 g         4790 t
ORIGIN      
        1 cggagatcgc ggcggccatg ctccagcgcc agcaggccac ggccatcatc gccgcccgac
       61 agatgatcgt ggagggggcc gtcgggatgg tggagatggc cctggagaag ctctcggaca
      121 agaacatcgt taacctggac gaggagcgga aggccgccat ggtcagcaac ctcctggtgg
      181 tcctgtgcgg caacaaggac gcccaacccg tggtaaactc gggctccatc tactgagata
      241 ttccggggga tcggcatgga cggcacggac aaggaaggcg gcaagaagca gatcctcttg
      301 aggctctccc cgagcctgtg gagggagctg gcggcctggg ccgaggacga tttccgctcc
      361 atcaacggcc agatcgagta cctcctgacc gagtgcgtcc ggcaaaggaa gaagaaatga
      421 gatagcgagg ggggcggccg ccggatcggg accgtccccc tgccggttct tgcgtttccg
      481 accgtagggc gtacaatgga ggcatcccgc atcgaggagc cttccatggc gctcaagcat
      541 atcggctacc gtgtcatcca gaacgtcctg cgcctgactt cctacttcat gtccttccgt
      601 ccgcccgaac tcctggaggg tcccggcagc gtcaaacgcc ttccggccct gatcaaggaa
      661 aagggctttt ccaaggtcct ggtggtcacg gacaagggcc ttcgggccgc gggtctcccg
      721 gacggcctcc tggccgggct ggacgagacg gggatcggcc gggtcctgta cgacggggtc
      781 cagcccaacc cgaccatcgg caacatcgaa gaggctctgt ccctcttcgt ccgggagggc
      841 tgccaggcgg tcgtcgcctt cgggggcggc tcccccatgg attgcgccaa ggcctgtgcg
      901 gcccgggccg cgaacccccg caggaccgta cgccggatgc ggggcctcct gcgggcgggc
      961 cggaaaccga agcccctctt cgcggtgccg accaccgcgg gcacgggttc cgaggggacc
     1021 gtggccgcgg tcatcgcgga cccggaaacc cacgagaagt attccatctc ggaccccaag
     1081 atcatgcctc gctgggcggt tctggatccc gagctgaccc tgggactgcc ccccaacatc
     1141 acctccacca cgggcctgga cgccctgacc cacgcggtgg aggcctacgt gggccggagc
     1201 aacaccgccg ggacccgggc catggccgag gaggcggtgc gcctggtctt cgcgaacctc
     1261 caggccgcct acaaggacgg acggaacctg gccgcccgga ccgccatgct ccgggcttct
     1321 ttctgcgcgg gctccgcctt cacccgggcc tacgtggggt acgtccacgc catagcccac
     1381 cagctggggg gcctgtacgg tatcccccac ggcctggcca acgcggtcat cctgccctac
     1441 gtgctggatt tctacgggaa cgccgtcgcc gcgcccctgg cccggctggc ggaagaagcc
     1501 ggggtcgggt ccgcaggcat ggacgaggag tcccgggccc gggccttcac cgcggccgtt
     1561 cgagacctgt cccggcgtct ttccatcccc gaacgtatgg acggcgtccg ggaggaggac
     1621 atccccctca tcgtacgacg cgccctggcg gaggcccacc ccctctatcc ggtgcccaag
     1681 ttcatggacg ccgcggactg cgaggcggtg gttcggcggg tggccgggaa atgaaggttt
     1741 ctggaatcac cacggagtga acggggaata ggccctggaa ccagaagatc gacaggatta
     1801 gcaggattcg gaatggatga acaggatgaa gagaggggga agatccattc cccctgatct
     1861 atcctgttca tcctttcttt atcttgttca tcctgtcccc actatggttc caaggcctgt
     1921 ctagaccaag cccaacccga cctcgagggc cttcaggttc aattccacga aggcgggttt
     1981 cacgttctcc cggatgatgc gggcccagtc gatggacccg agtcccatgg accgaattgc
     2041 ggcccccagg aggatgacgt tcatcacctt gggattcccg agttcggaag ccttggccgc
     2101 ggcgtccacg acggaggcgt cggccttgga tttgagctcc tccaggatcc cctcggggta
     2161 ctcggccctg ccggacacca cgggcatcgg gttgatccgg tagtcgttga cgatggcccg
     2221 gcccccgggc ttcaggaagc ggagccagcg cagggcctcc atccgctcga agctgaccag
     2281 gatgtccgcc ccgcccacct cgatgaccgg ggagtgtacc ttctcgtccc cgtaccggac
     2341 ctggctggac accgagccgc cccgctggga catgccgtgg atctcgctca tcttgacgtc
     2401 gtagccggcc tccatgaggc ccgtggtcag gagcttggcg gccaggatgg tgccctgtcc
     2461 gcccacgccc accagaagga tgctcttcac catgacggcc tcctcattcc cggacgatcg
     2521 cggacttggg gcagatctgc gcgcagacgc cgcagccgac gcactggtcc gggtcgatgc
     2581 cggacttctt cccggcgaag tcgaaggcga gggcgggaca gccgctccgg aggcaggcct
     2641 tgcagcctat gcacagatcg cggtccaccc ggtagcggga ctggaaggcc cccgcgaact
     2701 ccttcttgtc ctcggggctg aagcgcttga gggcgcaggg ccagcgggtg atgatcaccg
     2761 agggctcgtc cagggccagg gcccagtcca gggtctcctt gaccagcttg aggtcgttgg
     2821 ggtcgatggt ccgcacgttg gagatcccgc aggcccggac cagggcctcg aagtccaggt
     2881 ccggggtgcg gctcccgtcc agcgcgtatc ccgagcccgg gttctgctgg tgcccggtca
     2941 tcccggtgat ccggttgtcc aggatcacgt tgaccgtccg gctccggttg tagcccacca
     3001 tcaggaggga gttgatcccg gtgtggaaga aggtggagtc ccccaggacg gagaccaccc
     3061 ggcggggctt ctcgtccttg cgcatgtcga agacctgctg ggccccgtgg cccgcgctga
     3121 tgctggcgcc catgcagacg caggtgtcca tggcgttgta gggctccgcg aagcccaggg
     3181 tatagcagcc gatgtccccg gagaccatca cgttccgccg ccgcccgagc tcgtagaaga
     3241 agccccggtg gggacagccc gcgcagaggg tgggggggcg ggccacgatc ttggccgggt
     3301 ccgcctccaa ggcgtcgaag caggagccga acacggactt ccggatgatg tccgaggtga
     3361 gctccccgca gaaggggaag accttcttgc ccaggcagtc gaagcccagc aggcgcacct
     3421 gggtctcgat gatcgggtcg ttctcctcga tcacgtagat cttcttcacg gaaccgcaga
     3481 agtcccggat cctccggacc ggcatggggt tcgtgaagcc cagcttgagg taggacgccg
     3541 tcgagccgaa gacctccttg gcgaagaaga aggcggttcc cgaggcgatg accccgacct
     3601 ccgacccgtt gtcctccacg tagttcaagg ggctggcctc ggaatagtcc gcaaggcgcc
     3661 tcatccggtc ctccaccacc aggcggcggg cccgggcgtg ggccgggacg gagacgtact
     3721 tgaccgcgtc cttgcggtac tcccggatgg cggcttcctc gcggtccccg cactccacga
     3781 tacccttgga atggctggtc cgggtggtca tgcgcacaag gacaggggtg tcgtagtcct
     3841 cgctgacccg gaaggcttcc ttgaccatgt cccggcattc ctggctgtcc ccgggctcga
     3901 agagggggac cttggcgaag ggggcgtagt tgcggttgtc ctgctcgttc tgggaggagt
     3961 gctggccggg ctcgtccgcg gtgatcacca cgaaaccgcc gttggtgccc gtgtaggcca
     4021 tgctgaacag gggatccgcg gccacgttga ggcccacgtg cttcatggcc gccagggacc
     4081 gggctcccgc gatggcggcg cccatggccg cctcgaaggc caccttctcg ttgacccccc
     4141 attcggcctt gatgtcgccc ctgtattccg cgatggtctc caggatctcc gtgctggggg
     4201 tgccgggata ggcggatgcg tacttcacgc cggcttccca ggctccccgg gcgacggcct
     4261 cgtttcccgt catcagcttc ttcatgtcgc ctcccatgct ttatggttcc cggcgcggct
     4321 cagggagccg gggacagagc tatcaaactg gccagggcga tggagtggag cttcccctcc
     4381 gccgtctcgg accgggacgt caggacgatg gggcaggcgg cccccaggac cagacccgcc
     4441 atcttggcct tcgcgtagta gaccagggcc ttgcccaaaa ggttgcccgc ctcgatgttg
     4501 gggaccagga agaggtccgc gtccccggat atctcgctcc gaatcttctt gtgctccgcg
     4561 gcccggcggc tgaccgccac gtccagggcg atggggccct ccaggatgcc cgcggggatc
     4621 tccccccggg cccgcatctc caccagggcc cgggcgtccg tggtggaggg catcttgggg
     4681 ttcacctgct cgttggcgga caggagggcc gtcttgggct cccggatccc gacccgggcc
     4741 agggctccga gggcgttcag gagtatggcc ttcttctcat ccagacccgg ggcgatgttg
     4801 atccccccgt cggtatggaa catcagcttg gaaagccccg ggacctcgaa ggccgccagg
     4861 tgggaaagga gggaaccttt gcggagcccc gagtcggggt ccaggaccgc ccgcaggaag
     4921 tcgctggtgt tcacaaggcc cttcatgagg gcgtccgccc ggccttcccg caccagggag
     4981 gccgctctcc gcgccgccgc tacctcgtcc ggctcgtgga cgatatccag ggagccgacc
     5041 tcggccttgg ccgcgatggc ctcgatccgg ccccggtccc cgaccaggac ggcgtccgcg
     5101 atgcccgcgt cccgggcggc ccagacggcc cggaggacgt cctcgtcctg ggccacggcc
     5161 acgctgatcc ggacctttcc gagcttacgg cagatcgcga gtatgtcggt aaagtttccg
     5221 aaggattcct gtgccacggc ggtgtccccc cgggcaagga tatacctgtt tttccggggg
     5281 cgctatggta ttccgaagca cttttcctat actgtattcc atattgcagg tttttaggta
     5341 gcttcacctg caaaacggag gtcaccatgg acgccctgga cgtacgcctt ctggacctgc
     5401 tccagcagga cgcccgcatg aagatcagcg agctatccaa gcgcctgaac ctgagccgtc
     5461 cgagcgtctc ggagcggatg gcccggctcc aggatgccgg gatcatagaa ggctactccg
     5521 ctcggatctc cctcaaggcg atcggccggg acaccatgct cctcatccgg ctctcgtccc
     5581 tgaaggtgtc cccgcggatc ttcgaggcca agatcgaggc ggaccaggac atcctggagt
     5641 gccaccgggt caccggggag gtggactacc tcatcaaggc ggcggtcagc aacatggacg
     5701 agatgcgtca cctcatcgac cgcctcatgc ccttcgggga tgtctccacc tccatcgtga
     5761 tcctgtcgcc cattgcccac cggcgcatcc ttccccgggc cgcggagggg cggcctgtcg
     5821 agtaggcggc ttctccggcg aacgggatcg ggcgcccgcc gcggcatcta ccgcgagatc
     5881 ggcacctcca ccggggcgta ccgggcgtgg atcgaggtga agtcgtcccg ccggccgtag
     5941 gccaggatga gcttgagact ggagccggcg gcatggggcg ccgcgggcag ggcgaactcc
     6001 aggaccgtct tcccggcggt ctcccggacc gcctgggcgg tgaggatccg ggccgggttg
     6061 ggggtatgcc tgcggccgga tcccgtttcc tcgctgaccg cggccgttcc cgcggcgtcg
     6121 tagcccagga ccatgaaggc cccgtccatc cgcagggaac caagaccgac cgagacccag
     6181 cccgtggtgg gggcttccag ggcggcgtag agggtccgtc cgtcggggga tagggagagg
     6241 tggaccgtca tgtccttctg ggcggaggtg taggagtact ccccggggct caccgtgccg
     6301 tccgccgccg gggttttcgg ggaaggcgcc acgccttgag cggccaaggg agccgcggcg
     6361 gccagcaccg cgaccgccag gaccgcgagc ttcaggatcc ttgttcgatt catttcgttc
     6421 ctccttcttt cggggctttt ttctccgccc ctccccgtcc ggtcagccgg taccgggcgt
     6481 acatcccctg ggacaggacg aaccagaaga tcagccagga gactccgatc gggttcatcc
     6541 cgaagtcccc ggtggacgcc agcaaggcgt ggatcaggac caggtacgcc accaggacga
     6601 aggcgttgtg caggtcccgg gccttcttgt aggtcatccc cgtatgctcg gtgaagtgct
     6661 tcttgaaggc cgccaggaag ccgatccggg acaggaccgt gttggccatg atcaccgcgg
     6721 cgaaggcggc caggaccagg aggatcgtga aggccccgat gcccaacagg gcccggaggc
     6781 tggtggtccc gaagccggcg gcctttttca gggtgaagtg ggccgccacg atcaggacga
     6841 gggctgcggg ggccgtgcgg tgcaaagcca gaagacccct ggcgcccagg gccttgaccg
     6901 cgaagcccgg cttggaggcc aggatgaatt ggtcgcagag caggacgaag gcggaaaccc
     6961 ccaggagcac cgaggcggtg tagccgtcca ggtaggcccc gacggactga tagtagaagt
     7021 acaggggcac cgcgaagctc accaggtatc ccaggtagaa cagaaccttc ttcattcttt
     7081 ggcctccttg cggggacgtg atttaataat cgttctcagg aggtactccg gtcaaggaat
     7141 accttcgtca caaggatcgt gacaccgggg tcaaattgcc agatcgcatc ccgggtacta
     7201 tagtgaaggg agggcgccgg gaaaccgggc gcccgccatc atccgacatc aaccttggag
     7261 gaatgccatg aaaggaaacg agaagctgct ttcggccctg aacgggctcc tggcggacga
     7321 gctcaccgcc atcaaccagt acatggtcca ctcggagatg tgcgacgact ggggttacga
     7381 gaagctccac aagcacttcg agaagcgggc catcgacgag atgaagcacg ctgagaagct
     7441 catcggccgg atcctcttcc tggagggcat tccgatcgtc tcctccctga acaagatcac
     7501 catcggggcc acggtcccga agcagctgga caacgaccac gccgcggaga cgggagccat
     7561 caaggcctac aacgaggcca tcatcctggc cggggaggtc aaggactacg cgacccggga
     7621 cgtcctccag tcgatcctga acgacgagga cgcccacatg gacggcatcg aagccctgca
     7681 gagcaagatc gagcagatga ccctccaggt cttcctgacg acccaggtcg gggaatgagc
     7741 gagtacctct ctaggggccc gggtgccgag gaccgcgccg gacggctgaa ggtcatcatc
     7801 gagaagttgg ccgccggtgc tccgccttcg gcggtcaaga aggaattcca cgatctaatc
     7861 aaggacgcgg acgcctcgga ggtagcctcc ctggagcagt ccctgatcga gggagggatg
     7921 cccgtggagg aggtccagcg gctctgcgag gtccacgcgg acgtcttcaa ggcggggctc
     7981 gagcgggggg agcgggcgga ccgcatgccc ggccacccgg tgcacaccta ccgggcggag
     8041 aacgtcgagg cccggaagcg tgcccgagct ctgtccctgg ccgccttcgc gggcggcatt
     8101 ccggcggtca aggccgccat ggacggcctc cgcccgatca tcgtccacta cgagcgcaag
     8161 gagaaccagc tcttccccta cctggagcgg accgggttca cgggaccctc caaggtcatg
     8221 tggggcaagc acgacgagat ccgagccgcc ttccgggacc tggatgcggc tctgggggcc
     8281 ggcgacgtcc gatcggcgcg tgccaaggcc cgggatctcg tccggcgcgt ccggatgatg
     8341 atcttcatgg aggagcggat cctccttccc aacgccctga aacgcctgac cgacnnnnnn
     8401 nnnngactgg gccgaggtcc gccgggggga ggacgccatc ggcttcgcct gggtcaagcc
     8461 cggcgcccgg tgggatcccg ccctggcggt gcaggcggcc gggggcggcg cgtactcgaa
     8521 tttcgacgcg gccctgagaa ccgccgcgga agcagcggcc gcgaaggccg gctcggaaac
     8581 ctccgagccc gaaaagccgg gcggcggcgc catcgccctc tccacgggat ccctgcctct
     8641 tgacgtattg gaccggatcc ttaagagctt gcccgtcgac gtctccttcg tggacgcgga
     8701 cgacaaggtg atgtactact cggattcacc ccatcgggtc ttcccccgga gcccggggat
     8761 catcggccgt tcggtccaga attgccaccc ccacaagagc gtcgcggtgg tggagaaaat
     8821 tctggaatcc ttcaagcgca aggagaagga caaggcggat ttctggatcg agatgggcgg
     8881 ccgtttcatc gtgatctcct acaagcccat ctacgacgac gccgggacgt acctgggcac
     8941 cctggagatg agctgggacg ccacggaggt ccggggcctc acggggcaga ggcggctgtt
     9001 ggactggtag cgcgcgaggg ggacggcaaa gccgtccccc tcgcggcaac gaggcctacg
     9061 gcttcgtcga tcttcaggag cctttggtcg gcaaagccga ccaaagggcg gcacggaagc
     9121 tcttcgagct tccgcgaatt taatcaacgc cggcttccgc cggcgcggca acgaatccgc
     9181 gtcatccctg acggttcaga atcgcgcagc gattctgtcc cacaatcaag cgcccatccc
     9241 tgggcgcgga tggtgcttcg tcgatcttca ggagccttcg gtcggctttg ctgaccgaat
     9301 cgcggcacgg aagctcttcg agcctcagcg acccaggggc gcccgatggc ccccttggat
     9361 tatgggaatc tttcctgata taaaaagtat aaaagtaccg caatacccta ttgacagaat
     9421 taaggcacga tagtaccatt ttaccaggaa cacgatgccg ccgctcgggc agacaaagag
     9481 gacggcggcc agaaggagcc atagatgaag aagcagatga ccgcgacggt gtcctgggta
     9541 ggcaaggtgg actgggagct cacgaagttc cacggggacg agtactccac ccacaagggc
     9601 tcgacgtaca acagctacgt gatcgaggag gggaagaccg tcctcgtcga cacggtctgg
     9661 aagcccttcg ccaaggagtt cgtggacaac ctggaggccg agggacttct gggcaagctg
     9721 gacgcggtga tcgcccagca cgcggagatc gaccactccg gggccctgcc ggagctcctg
     9781 cgccgccgtc cggacctgcc cgtgtactgc accgccaacg gcatcaagtc cctgaagggc
     9841 cactaccacc aggactggga cttccacccg gtcaagaccg gggacgagtt cgacctgggg
     9901 aacggcaaga agctcgtctt cgtggaagcc cccatgctcc actggccgga cagcatgttc
     9961 tcctacatga ccggggacgc catcctcttc tccaacgacg ccttcggcca gcacctggcc
    10021 accgagcgcc tctacaacga cctggtggac caggacgagc tctacgacga atgcgtcaag
    10081 tactacgcga acatcctgac ccccttctcg gccctggtga aggccaagat cgagcaggtt
    10141 ctgtccctca agctgccggt gaacatgata gccaccagcc acggggtgat ctggcggaag
    10201 gatcccaccc agatcgtgct caagtacatg gagtggtgcc aggactataa ggaggaccgg
    10261 atcaccatcc tctacgacac catgtgggag ggaacccggg ccttggcgga cgagatggcc
    10321 cgggccatcc aggaggcctc gccctccacg accgtggttt cccactgcct gtccaagttc
    10381 gacaagaacg acgccctcac agacgtcttc cgctccaagg cgatcctcgt cggctctccc
    10441 accatcggga agggcatcct gaccgccgtg gcgggaactc tcgaggagat caagggcctg
    10501 cggttcaagg gcaagaaggc ggcggccttc gggtgctacg gctggtccgg ggagtccgtg
    10561 aagatcatcg gcgatgccct ccgggaatcc ggcttcgaag tggtcgacga ggggatccgg
    10621 gccctgtgga atcccgacac ggacgccttg aaggccgccc gggactacgg caaggccttc
    10681 gcggccaagg tgaagtagac ggaggaggcg actgcccggc cccgggtcgt atcctgacgg
    10741 aggcgtgccc ctggcggttc gaagcttgtc cggggagacc ggcgaaagcc ggactccccg
    10801 cggcgaagcg gacatcggcg atcgaccggg tggatcggga gccgtcggtg tgaagcccgg
    10861 cttggctccg ggatccggtg aaacggctgc cggaaagaca ccttgacaaa taatatatag
    10921 atagactatc taaacatatg gttgttgaag aacccgtcga agatcgcttc gtagaagctc
    10981 tctgcgtact gcgagcccgg atcttcgaag gatccccggc ggagagctcg gggctcaccc
    11041 ccgccctggc caaggccctc ctggcgatta cggatggagg agaaaggcac ctgcgcgtgc
    11101 gcgagctcgc ggaatccctg ggcatcaagg aatccagcgc cagcgtactg gccgacaggc
    11161 ttgtccaggc aggacttgca aacaagaaac cggacccggt ggatgcgcgt gctcttttag
    11221 tgggagcttc tccctcggga tccaagcttg ccgaagccgt acgccttcac aggcgcagaa
    11281 tagcacgcag cctgatggaa gtgctgtcta gggaggaatc tcaaagactc ctggaatact
    11341 tggaaaagat gggcaagagg gaggaatatt gaactcgacg gcgatcatta tcaacggttt
    11401 ggctgtcgca gccctgatat gggccttggt cggcgaccgg gaaaaagccc gggcgggtct
    11461 gaggaaaggg ctgcaggtga tgatggggat cgctcccgta accctgctgg tggtggcggt
    11521 ggtgggcatc gtcctggctt tcatccccgc cgagaccgta ggactggttc ttggcgaagg
    11581 tgcaggattc acgggtgtcc tcgcagccct cgggctggga gccttggttc atatgcctgc
    11641 cctgctgggc ttccccctgg ccggcaaggt cctccaaaac gggggatccc cggggcccgt
    11701 agctgcattc ataacctccc tgaccatgat cggggtattc accattccga tggagatcaa
    11761 gatcctggga aggcgtttca ccctggcccg gaatctggtc agcgcggccc tggccctgtc
    11821 catcgccgtc ctgatgggag tccttgtatg aacggggaat cgaaaagccc gggtacgggc
    11881 gttgaggctc ctaagcggag taaagggctt ctcctcgcct tcgctgctct ggttctgatc
    11941 ggaggattct cccttgcatt cccggataaa cgtgccgcga tcctcgaggg aagcggcgga
    12001 ttcctccggg aggtcgtgtt catccttccc gcagtctgcg tcctcatggg gctcttcgga
    12061 gccttcgtgt ccaaggacct ggtgatgagg atcctgggga aatcctccgg ctggaagggg
    12121 gcgcttctct ccatcggtct gggctcccta cccaccggtc ccctgtacgc ggcgttcccc
    12181 cttgccgcag ccctccggga aaaaggggcc cgatccgcga atatcttcct gttcctgacg
    12241 gcctgggctt gtatcaagct tccacaggag cttctcgagc tccgcttcct gggtccccgg
    12301 ttcatgctga tccgactggc cctgaccgtc gccctggccg tacccgcggc cttcctcgtg
    12361 gaacggtatg tggattccgt cgcgtcccgg tcggaaccgg aatcacctgc gacttcaaac
    12421 ggaaaggaga tgcgaccatg atcgagagtg cttttccatt cgcccgcacc gattcccaga
    12481 ccatggagaa gatcgtggac gacgagcggg tcaatatcaa ccacatcgtc gtggagcccg
    12541 gcaaggccgt ccccacccat gtctccaact ccttcgtcca ccaggtggtg gtgcggggga
    12601 ctttgagcct ctccctggag gacggtcccg caaccccgca tcccgcgggc accatcgtgg
    12661 ccgttccctt caacacgaag atggccatcc agaacgaggg gaccgagacc ctggagttct
    12721 tcgtcgtgaa ggccccgaac ccccgggaca tgcccccggt caaggcgatc tagcaggttg
    12781 ttgaaatact cgaagcattt caacaacctg ctatgcaatt gctcgagaac cttacggttc
    12841 tctgcgcaat tgcgacatta aggtagtgtt gaaaaaggct ccttagcctt tttcaacacc
    12901 ctgctagaga ggccgacaac ctcgaggccg cttggcatcc tgccggcggg accttcgcgg
    12961 ccgcggggcg ctcctacagg aaggaacgcg ccttcaggaa ctcccgcatc tcctcgaagg
    13021 cggccccgct ctcggggacc aggtccccca gggcgggcca gacatgccag agcccgtccc
    13081 agacgctcaa gctcaggtcc acgccggcgg cctcggcctt ctcggccacg gtccggctgt
    13141 cgtccaggag gatctccccc cggtcgacct ggatcaggag gggcggaagg ccccggaagt
    13201 ctccgtgtac gggggagatg tagggatcgt cgagacgggc gccgcctgag tacaaggccg
    13261 cccaccgatc aagcgtcccg gtgtccagga gggcttccga cttcgcgttg gcggcatggg
    13321 acgccccgga gttcgatagg tccgcccagg gggacaggca gatcaccgcg gcgggcgcag
    13381 ggctccccga atcacggagg gacagagccg cggccaggca gaggcccccg ccggcggaat
    13441 cccccgccag gacgatgtcg gaagcggcat ggccctccgc caggagcccc cggaaagcct
    13501 tcaacgcgtc ctcgagagcc gccgggtagg gatgctccgg ggccaggcgg tactccggca
    13561 ggacgaccct caggccgagc gcccgggcca ggggcacgca gagcatccag tacgactcga
    13621 tcgagccggt cacgtacccc ccgccgtgca ggtagagcac taccttttcg gggcgctccc
    13681 ggggcggccg gatggccgca cagcggagac cttccaggac aatctcctcg atgcccgtgt
    13741 ccgtcgggaa gggcgggtgg aagcggccgt tcctctcgga acgggctcgc agttccggaa
    13801 tggacagccc cttgtcccgg aacgccgccc ggatcgcggc cttccagaaa cggctgcgca
    13861 ggcttgcgga catggatgcc tccgatggtc ccggccgagg gacagaggat acgagtctgg
    13921 acgccgccgg gccccgcgtc aagcggagat ttgcgcgttc cgccgtccga aaccgcaccc
    13981 gccaccggcg gctcgaaccg cggcaggtgg aggggagctt ctatcaaaat gttatccccg
    14041 tttcatcccc cgtttatggg aacgctccgg acaaatccgt atcgttctcc cgtgacgcga
    14101 gggccggccg acgacatcga atcggtacgt acaccccgcg ccgcgaggag aacgcaatgg
    14161 caatcctgca gctgtccgac gtgcacaaga cctatcccct gggcaagacc gaggtccacg
    14221 cggtccgggg cgtgagcctc gagatccaga agggggactt cgtatccatc gccgggcctt
    14281 cgggctcggg caagtcgacc atcctcaacc tcataggctg catcgaccgg cccagcgggg
    14341 gctcggtccg cctgggcggg gtcgacacgg ctaagctgtc cgaccgggag ctcacccggc
    14401 tccggcaccg ggtcctgggc ttcatcttcc agtccttcaa cctgatcccc gtcctggacg
    14461 tatacgagaa cgtcgagttc cccctcctcc tggggcgcga ggtgcccaag cccgccgagc
    14521 ggcgggagcg ggtgggccgc ctcatccggg aggtgggcct ggagacgtgg actcgccaca
    14581 aggccaacga gctttccgga ggccagcgcc agcgggtggc gatcgcccgg gccctggtca
    14641 cccggccgga ggtggtcctg gcggacgagc cgaccgccaa cctggattcc gccaccgggg
    14701 aatcgatcct ggccctgatg caggccatga accgggagtt cggcaccacc ttcgtcttca
    14761 gcacccacga cgcccggatc gtggacctcg cggaccacgt cgtccggctg aaggacggcc
    14821 gcatcgccga ggaattccgg cggggaggcg cggcatgatc ctgctgcggc tggcgttccg
    14881 gaacatgagg gagcaccggg ccaagaccct gatcgtgggg gtcctggtga ccctgggggc
    14941 cttcaccctg acggcgggca actccttcct ggacagcctg cgggccggcc agcgccgaac
    15001 cttcgtggag agcttcaccg gagacctgat cgtccgggcc gaatcggacg aggcctactc
    15061 cctgaccgtg agccccgccc ggtcccccgc cccggaactg ccggggttcc cggaggtccg
    15121 ggagagggcg gaagccctgg acggcgtcgc ggcagtcctt cccctggtga actccatggc
    15181 caccctctcg gcgggagaga cggccgcggg cttctccatg ctctgggggg tggacttcgc
    15241 ggagtaccgg gcgatgttcc cggagagcct cgagctggtc gagggggact ggcccgcgga
    15301 gccgggggcc tggatcctgg tctccgaggc cacccgccgc gaggcggagg cggaggccga
    15361 ggtcccggtc cgcgtcgggg accgcctcct ggttaccggc atgggggagg gcggcacgaa
    15421 gatccgggaa gtaaccctgg ccggggtgtt ccggttccgc cgggggaata acctcttgga
    15481 ccggatctcc ctcacggatc ccgggaccgt gcgctccctg gcgggcctga ccctggcgcg
    15541 gagggaagcg gcgtcttcgg agtcctcgga gacgggggcc gatgccgcgg tggccgcccc
    15601 ggccgtcacg gacgaggatg cactcttctc ggggttctcc atcgccgcgg ccgagacctc
    15661 ggccggtccg gcggacttcg attccatcct cggggacacg agcgcccggg cccggtactc
    15721 ggccgtggac tcggacgcct ggaacttcct gctcatccgg ctcgcggacg gccgggacgc
    15781 gggacgcgcc gcggaggagc tcggcgcggc gatcggggcc gggacctccg ggctcgtcgt
    15841 ctccgactgg agctgggggg cgggggtcca ggcggacctc tcccggtacc tccggctggc
    15901 cttcaacgga atcgtcctca tcgtcgtggc cgtggccgcc atcatcataa tgaataccct
    15961 ggtcatatcg atcaccgaac gcatccccga gatcgggacg atgcgggcca tcggggccgg
    16021 gcgcgccttc gtccgccgga tgatcctgac ggagaccctc atggtctccc tggccttcgg
    16081 ggccctgggc gtcctggcgg gctgcgtcgc ggtggggatc ctgggccggg cgggcatctc
    16141 cagctccaac gagatcctgg cgaccctgtt cggcggggag gtcttccggc ccgccgtatc
    16201 ggcggccgcg gtggccggcg ccctggcggc catggcgatg gtgggggtgc tgtcctgcct
    16261 ctaccccgtg tccgtcgccc tccggatcac ccccgtccgg gccctgcaga aagcctagag
    16321 gagaacgatc gtgaaccaat ccatgcgcat agccttccgc aacgtggggc ggcagaagaa
    16381 gcggaccgtc ctgttggccg gggccgtcgg gctcggcttc ttcatcatca ccctgtccgg
    16441 gagcctgctg ggcggcatcg ccgcgtcggc ccggaagaac ttctccgcgg cgttcggggg
    16501 tcacctgtac ttctccggct ccgtggtgtc ggaccgggga tcggagatcg ccgtgatggc
    16561 ggatccgggt cccctggagt cggtcctggc ggcccatgcc cgggacatag cgtcgatcca
    16621 ccggagatcc gggacgtcgg gcaccctgat cttcggtacc cgggagctgc ggcagacctt
    16681 gaccggggtg gacttcgggc gggagcccga tttcgccggg ggcctgcgga tcgtctcggg
    16741 atccctggac ggattgtccg agccgggggc cctggtcctg ccggaagacg ccgcggaacg
    16801 cctgggcgtc cggacggggg aggccgtgct cttccgggcg tccaccgtcc acggccagcg
    16861 gaacgtcacc gagctcgtcc tgatcgcgac ctacgagggg cagtcctcct tcggggtcag
    16921 cgccgggtac gccggcctgg ggaccgtcaa ctccctcctg ggactcggac ccgaggagtt
    16981 ccagaccctg aacgtctggc tccgggacga ccgggccatc gacgcggcgg ccggacgcat
    17041 cctggcggac ctggcgggga aggtcccggt ggagccgcgg gaggacgagg atcccatgaa
    17101 gcggatggcg gaacggctcc tgggcggctc gggtccccgg agcgtcgggg acggggaggg
    17161 ctgggagggc acgcggtacg gcctcacgac cctgaacgac ctcatggccc agttcacctc
    17221 gatcctgtcc attgcggacg ccctgtcctt ctgggtcttc ctgatcctcc tgtccatcac
    17281 catggtgggg atcctcaatt cctaccgcat ggtcatggtg gagcggacgg ccgagatcgg
    17341 gaccctccgc gccctggggc tccagaaggg aggcgtgcgg gacatctttc tctgggaggc
    17401 cctcttcgtc tccctgtacg gtgcactggc ggggctggcc gcggccctgg ccgtcatggc
    17461 cctctcccgg gccgcggtcc ttccgggcgg cacggtgctc tcgttcttcc tggactccgg
    17521 acgcctcccg ttctccgtgg atccgatcga gacggtccgg aacctcgtcc tcctgtgcgc
    17581 ggccagcctc gcggccgtct cgttccccgc ccgcgcggcg gcccggcttc cgccggccgc
    17641 ggcgcttcgg gccgcgtact agcggagccg gcggtttccc gccgatcccc cggcccgggg
    17701 gtatcgggct tcgattcgac ctgtgtcaaa ggagcgcttc atgagactac gaacattcgc
    17761 cgcggccgcg ctggtcctgg catccttcct gcgggcggcg cccctggccg cccaggcctc
    17821 cggaaccccg gacttcaagg ccctcctcgc ggccgtcgac ggccggacga acttccgggc
    17881 atccgatttc tccgcccgcc tcaccctggt ctccgaggat ccctcgaagg ggaccgagac
    17941 ccggagcgtc cgggtcttcc ggagggaccg ggccgacacc ctccttttac tggtgctcaa
    18001 gcccgagagc gagctgggcc agggttacct gcgggtcgac gactccctgt ggttctacga
    18061 ccccgagagc cggaagttca cccacagctc cgtcaaggag aacttccagg gcacggacgc
    18121 gaagaactcg gacttcggcc tttcctccct ggccgaggac tacgcggtcg ctgcgcacgg
    18181 ggagggcagg ctcggcgccc acgagacctg gatcctggac ctcaaggccc ggcacgacga
    18241 gacggcctac gcctcccgga aggtctggat caccaagaag gacggcctcc tactgaagag
    18301 cgaggactat tccctgtccg ggcgcctcct gcgcaccgcc tactatacga gctacgcccg
    18361 ggtggggtcg tccctgatct gcgaccggtt cacctacgtg gacgccctgg tggccgggaa
    18421 gaagacctcc gtcctcatgg aggagatcag tctgcgcccc gttccggacg aggtcttcac
    18481 caaggcctac gtcgagcggg tgaaccggta gggggcggag catggacacg acgcgattcg
    18541 ggcgggccct ggccgccctg ctcctggccc tggcggtccg gaccgcctcc gcccaggcgg
    18601 ctccgtcccc ggaacaggaa atcccggcag ctccgtcccc ggcggacgag gagttcttcg
    18661 gggccgggga cgacctggtg accgaggcgc ccgaggccgg ggacgcggat tccggaggag
    18721 ccggccttcc ggagttccag aaggtccgat ggggcgggac cttccgcctc gaggccgcgg
    18781 gcacggcgga cgccgaggcc ctggcccggg gcgagtgggc cggggctccc ggcgcggagc
    18841 tcgacctctc gggcactttc ttcctggacg cccggccgag cgaggaggtc cgcgtcctcg
    18901 ccaaggcccg ggcggcctac cccttcgagg acgccgggga ctgggccctg cgggaactct
    18961 tcgcggacct cgccctccaa cccgggctcc ggctccgggc gggcaagcag acggtcaact
    19021 ggggggtcgg gcggttctgg agcccggcga acgtgatcaa cctggagcgg atcgacccgg
    19081 aacacccgga cgaggagctg gccggccccg tggcggcccg gctccacgcc gtccggggca
    19141 cctcgaacct ctacgcctac gccctgctcc agggcatgga ggaggacggc accgtcggga
    19201 tcgcggccaa ggccgagggg cttgtcgggg ggacagagct gtccctggcc ggggtctggc
    19261 ggccggaccg gccctgggcc gtcgcggcca cagccaccgg gcccgccctc ggtctggacc
    19321 tctacgccga ggcggtcgtc cggggcggag gaagacggac ctacgtggtc gaggacgggg
    19381 gggcgcccct gggggtctcc gcggagagcc gggaggccga accggccttc cagggcaccc
    19441 tgggcttctc ctggacctgg agcgacgaga cggaggcctg gaacctcgcg gcctcggccc
    19501 agtacctccg gaacggggcg ggctacgccg atccggacct cttcacgacc tacgcggcgg
    19561 gggtggccgc cctggccgcc gcggggcaga tcggcccgga ggacctctac ctccggggaa
    19621 ggaactacgg ggccgcgagc ctcgcgctct cggacatagc ccggagcgac gcggggctct
    19681 ccctgctctg gctgggcagc ctggacgacc ggtcgggcaa ggtcgtcgcg tccgcctcgt
    19741 actcgggttt caagaacctg cggattgcgc tatcctacgg ggagcgctac ggaagggcgg
    19801 ggagcgagta cgggttcccc ggtccccggc gcacgctggg tctttccgtc gagacgacgg
    19861 gactctcgtt ctaggaggac accgagccgt ggacgcccgg atactggtga tcgaggacga
    19921 gcccgacctg gcccgcctcg cggggatgta cctggagcgg gagggcatga cctgcgccct
    19981 ggctccatcc gccgaggacg ccctcgtccg gctcgcctcg gagcgcttcg acctcgtgat
    20041 cctggacatc aacctgccgg gcatggacgg cttcgagttc ctccgccgcc tgcgccgcga
    20101 cgaccgggtg cccgtcgtgg tcgtgtccgc ccgggaggcg gacgaggacg tggtcctggg
    20161 cctgggcctg ggggcggacg agttcatcgt caagcccgtc gccccccggg tcctggccgc
    20221 ccgggtccag gccctcctcc gccggctccg ggaagggtcg acggcgcgga ggctcctgcg
    20281 cttcggggac ttcgagctcg atccggaagc ctgcgtcctg actcggggcg gccggagggt
    20341 cgggctctcg ggcaaggagg tcgacatcct ggtcttcctg gcctccgcgg gcggccgacc
    20401 ctccacaccc aaggagatct acgagaaggt ctggggccag gtctacgggg acctctccat
    20461 cgtgggggtc tacatccagc gcctccggcg caaggtggag gaggacccgg cggccccccg
    20521 attcctggag acggtctacg gcaaggggta ccgcttcaat cccggggagc tcaagagggc
    20581 ggggccgtgg gactgaaggc ccgcctcctc ctcctcatga ccggcatcgc cgtcctgccg
    20641 gtcctgagct cggccctcct catcctgtac ctgcggaccc agtatccggg cttcgccgcc
    20701 ctggactcct cgcccctgga gctgggcatc cggcagatgg aaacccggct ggccgaggcc
    20761 ctcgaggaag gcgacctgga gcggttcctc cggggttcgg agaacctctc gatccgggtg
    20821 gaggagtccg ataccggccg gatcgtcttc gagcgcgcgg ttcccgccgg cgggccgggc
    20881 gcggacgagc tcgcgatccg cttctccgcg ggcggccggg ggtaccgggc ggtcttccgg
    20941 tcggactccc ccccggacga gctcggcccc tccctcatcc tgcccctcct gcccgtggcc
    21001 gtcctgggca tcgtgatcct cttcaccgtg gccgtctcct tcgggatcct ccggtccctg
    21061 gggcggtcca tggggaagct ggaggaggcg acgcggagga tcgccgcggg ggacctggat
    21121 ttcgagccgg cccttccggc cggggattct ctggcctccc tggcggcgtc catggacgcc
    21181 atgcgcctcc ggctcaagga ggagtaccag cgccgggacc gcttcatcct ggcggtgtcc
    21241 cacgacctca agacccccct ggccgtcctg gagggctatc tggacgcctt cgcggacggg
    21301 ctcgcggaca gcccggagaa acgggagtcc tacctgaagg tcctgcggga gaagacgggc
    21361 gtcctggcgc accggatctc ccacctggtg gagctggccc ggatgaccac cgtggaatgg
    21421 aggcagacct tcgaggactg cgacctttcc gccttcctgg aggagaccct cggactcctg
    21481 gccgacgagg ccgcggcccg gggccgccgg atggaggtgg acttccgcct gtccccgggc
    21541 ctgcgggccg ccctgaaccg ggacatggcg cgccgggtcc tggagaacct ggcggacaat
    21601 gcggaaacct actcgccccc ggactcggtc cttcgcgcct ccgcccggga ggagggggcc
    21661 gaggtgctcg tgcggatctc gaacgagggg gagggcatcc ccgcggagcg ccggggcctg
    21721 gtcttcgagc ccttctaccg ggggaatccc ggccgcaacg ccgggggctt cggcctcggg
    21781 ctcgcgtccg tgaagtccat cgcggaggcc cacgggtggg ggatcgacct cgagtccgtt
    21841 cccgggggga cgacgacctt cacggtacgg attccggcgc cgcggtccta ggagcctgtc
    21901 gggagctccg gcaccacccc cgccaaccgg tcccagtccg gctcgggcaa gtccgtgggg
    21961 tacaggccgt cgatccggat cagcacgagg ccgtggatgt aggcccaggc cgcggcggtg
    22021 gcgtccaggt ccgggggacc ggcctggcgg acgtcggggc ttccctccgc gccggtccgg
    22081 gcggcctcca gggtccccgc caggagcata agggacttcc gggcccagcc ggtatattcc
    22141 tggggcaagc ggcacatcag gggtgagttc atgagccggt agagggccgg ccgggagacc
    22201 gcgaaggcca tgtaggcccg gcccatggcg ccgaccgggg tttcggcgcg ccgccgcttc
    22261 ctgcccggag ctatggaggc cgccagggct tcgtgcaaga gcctgtaccc ctcgtcggcc
    22321 agggcgccca ggaactcctc cttgtccttg aagtgccggt acggggccgt cttggagacc
    22381 cccgcggacc cggacagggc ccggaggctc agggcctcgg ctccttcctg ctccagggcg
    22441 tccagacccc gccggatcag ctcctcccgg aggtggccgt ggtggtaggt gtccttcctc
    22501 gtctccgttt cgctcattcc gcgagtcccc gcttccccgt ccagtctatc gtctcggccg
    22561 gcgcccggtc aaagggagtc cgtccccgcc gaccgcgcga aaacttcgcc gagtcatgtt
    22621 gacactgcca acacttgtca gtataatgtt ggaagtgcca acaatcaaga ggattcggca
    22681 cgaaggagag atcatggttg acagcaacat gaggaagacg ggcgggatcg ccgtcttcgt
    22741 actggccggc ctgttcacgg cggccttcgc gggcgagccc gccgctctgc ccgcccccaa
    22801 gcccgggacc gtctcggggc agcgcctcga gctccggctg gagccgggcc cgcactacgc
    22861 caaggacatg cgtatgctgt ggatccagta caccgtatgg ccccaggtgg ccgcgtggat
    22921 cgagactccc gacggccgct tcctggacac cctctacgtg accgaactgg ccgtgacggg
    22981 caagtaccgg gcggcgccca agagcggccg gcccgaggcc ctgcccgtat ggtccgcccg
    23041 gaagtccggg gccgcggacg ccgtgtcctc cccgaccacc gtcgggtccc cggtcgtgta
    23101 cggcaacggg atcgcctccc gcctgcccgc gggacggtat gtggtgatgc tggagacgaa
    23161 ccgctcctac gactggaacg gcacctacac caagaagaac tcgggcgtga acggccaacc
    23221 ctcggtcgtg taccgggcgg agctcgagat cggctcggga ccccgggagg cccggctcac
    23281 cccgatcggg atcggctccg tggacgggtc cgacgggaag gtccggccgg gcctggacgg
    23341 gatcgacacg gccctcgagc tgttctccgc gatgacggtt aagtacctgg acaagtaagg
    23401 actcgggact cgggaggggg gagggcggcc gctcgggtcg ccctccattt tttacgcgag
    23461 cagccggagg ttcgcctcca gggcggggcc ggacatcaag tccgtcaagg cggtccgggc
    23521 gtgctcaagg cccagccccg ggacgaggcc ttcccggcag atcgccgcca ggacggcggt
    23581 gttctgcatc cggggatccg ggaccgcgtc cgcgggcacc cggacggccc ggccccggcg
    23641 cagggcggcc acctcctcca cgtcggaaac ctccgcgggc cgctcggtgc ccaggcggac
    23701 ggccaggggc tgccaggagg cgtcgcacca gaccagggtc ccgccctcct tgaggtaggc
    23761 ccggcccgcg gacacggcct cgtggatctc cagggcgacg accaggtccg cggaaccctc
    23821 ggagaccagg gcggagtggc agcgggaccc gatccggaca tggctggcta cggagccgcc
    23881 ccgctgggcc aggccgtggg tgtcgcagcc ccgggcggtc aggcccgccg cgtcggcggc
    23941 gcggatcagg gcctccgaga ggaggccgat cccctggccc cctacccccg cgatgtagat
    24001 gtcgaagcgg tcggtcatcg cccttcctcc ttgtcctgga ccagggcccc ggcggggcag
    24061 gtctgccggc aggagccgtc cccgatgcag aggtcccggc tgacctccac ggccccgccc
    24121 tctcgggcct ggaagctcgg gcaggcgaag cgctcgatgc actcgtggtt cttcacgcag
    24181 gtgtccggat cgacccggac ttgccggacc ttccatgcgg ccccggcccg ccgccgggcc
    24241 tcccgggtga gcttcaacat gcaggggtgc cgggcgatga tcaccttgaa gcccggttcg
    24301 gccagggctt cccggaccag cgcagtcagc tcggcctggc ggtaggcgtc cacctcgcgg
    24361 atgtttttca cccccagccc ttccagggcc gcccgcaccg agagcttggg cgccgggccg
    24421 ttgaaggacc tgccggtgcc ggggtggtcc tggtggcccg tcatggcggt ggtcccgttt
    24481 tccatcacca ccaggaggac ttcgtggccg ttgtacacgg cgttgacgat cccgggcatc
    24541 ccggcgtgga agaaggtgga gtcccccagg aagcagacca cccggcggcc ctcccggaag
    24601 atcgagagcc ccgaggcggt ggaggtggag tggcccatgg acagaaggac tccgcccatg
    24661 cggtagggcg ggaggtagcc cagggtgtgg cagccgatgt ccgccaccgt gacgtccccg
    24721 ggggacagag ctcgcttgat cgcgtaaaaa gccgagcggt gcccgcagcc cgggcacatc
    24781 tggggggggc ggggggccgc cgaagcgggt aggggggcgg gccgaggcgc gtcgaatagg
    24841 tccggccagg tgcccgaaag gatgtcccgg accttggccg ggacgcactc gcccatgagg
    24901 tcctcgacgc cccgcttgcc caggatccgg gtcgataggc cctcgtcgta ggccagggcc
    24961 ttgatctcgg tctccagcac cgggtccagc tcctccagga ccttcacttc ctcgcgggca
    25021 cggaggaagg ccagcacggc gtcccgcggc agggggtgga ccagtcccag cttgaggaca
    25081 tccggccgct ccgatccggg tttcgtctcc tccagggcgt cccggaggga gagccaggcg
    25141 gtacccgcgg tgatcacgcc ccgccgggat ccggagcctt ccacgaagtt cgttcccagg
    25201 gcctcggggt cccgaccgat cgccgcaagt cgctccaggg cccgcctctt caacggaaag
    25261 accgtggccg ccagggggat gtagttctcc cgttccgggt cgaaggccca ggcgggccgg
    25321 gggaaggcgg gaggcagggc ctcgaagtcc accttttcct tggcatggca gacgtgggtg
    25381 gtcaggcgga ggatgaccgc ggtgcggtgg cgcgcggcca gccgggcggc ggtcttgaac
    25441 atgtcatagg cctcctgcgc cgaggccggc tccaggaggg gcatgtagct catccgggcg
    25501 tagtggcggt tgtcctgctc gttctggctc gagttggccc cggggtcgtc ccccaggatg
    25561 accacgaggc ccccgggtat gtccaggagg ggcagctgga cgaaggagtc cgccgccacg
    25621 ttcagtccca cgctcttgaa gaagaccgcg gacgggcggc ccgcaagcgc ggatccgaag
    25681 gccacctcca gggcgacttt ctcgttgacg gagaactcga agcggaaggg gcgccggtcc
    25741 gcggggatgg ccgcgatggc gtccgcgatc tccggggtcg gggagcccgg gtaggccgtc
    25801 acggaccgca gccccgattc gaccagggcg cgcacgatcg cggtgtttcc gatgactatc
    25861 tccgagaagg gttcctttcg gagcagggtc tcgccgagat ccttcatggg gcctcccctc
    25921 ccgcgggcga accgcgggaa ctccagtatc gcacggcccg ggcgcggggg gaaggcggat
    25981 tcgagctctg tcccccgaac tcgagctctg tccccgaact cgagctctgt ccccggtccc
    26041 gccgatctcc cccgttgaca cccgggggcc ggaatcccga gaatcagacc atggccaagt
    26101 cgaccaagcg gaagtgcaaa ttcgatcccg agcgacccta ctgcctggtc ctgggcggcg
    26161 ggggggccaa gggggtctac catatcggag cctggagggc tctccgggag ctgggcgtcc
    26221 ccgtgaacgc ctacgtgggc aactccatcg gggccatcat cggggccttc ctggcccagg
    26281 ggctcgagaa ggagctggag gagctcgggg cgaccattag gctcgagtcc atcgtggacg
    26341 tgggacccga gaaggacggg ctgccggacg gatccgagat ggtgtcccgg atcaagaagg
    26401 tctcccgaag cctggcccgg aaggggggac tggacacctc gcccctccgg tccacgctga
    26461 tggagctcat cgacgaaaag gtcatacgct cccggggctg cgacctgggc gtcacgaccg
    26521 tgaacgtcag cgacctggaa cccgaggaaa tcttcctgga ttccatgccg ccgggaagcc
    26581 tggtcgacta cctcatggcc agctccgcgg tgccgggctt cactctgccc aagatccagg
    26641 gcaagaccta cgtggacggg ggagtctacg acaacgtccc ctacgagatg gcccggcgcc
    26701 ggggataccg ccggatcatc gtggtggaca tctccggggc ggggatcaac ctgaagcccg
    26761 acgtggaggg ctccgagacg gtctacatca agaactccat ccagatgggc gggatcctgg
    26821 acttcgaccg ggccttcctg gaccgcttca ccctgttggg ctaccttgac acgatgcggg
    26881 ttttcggccg gctgtccggg tactcttact tcctccgccc ggatccggaa gcggaggccc
    26941 ggtttcgcgg ggaagccgcc cggttgggcc ctcccctggc cggggaagcc gaacgtccgt
    27001 ccaaggactt cttcccggac cggatgcgcc tggacggtcg cctcctcctg aaatgcctgg
    27061 agtgctcggc tcagtccctg cacgtggagc ggatccggga atacacctac gccgagctgg
    27121 cctccgaggt cgcgcggaag agggccgagg aggaggaacg ggcgaagcac gccatcgggt
    27181 ccggggaagc ccggggggcg cgccgcctgg agcaggtcct ggccgcggcc tggaaggacg
    27241 gccgcttcga gaagccgccc tacttctacc tcctcctcgc ccgggccacc cccgccggaa
    27301 aggcccggga ggcggtgcac atgctcctgg acaggttctt cccggagctc aaggccgcgg
    27361 aagcctggtt cggggtggac ccgaggattc tggaagaggt catttctcgc tgaagaccgt
    27421 gactcccttt cagctcgccc gaatgccttt ctgggctgga aaaggggaag ggagaacaag
    27481 gccgtgaaga cgagatgggt gttacgcgtc gacggctttc cagcgcgggg agtcgtgccg
    27541 attcagggcc tgtcccccag cctgcgccgg cccaagttcg cgccgcgctt ggcggccatg
    27601 cgttcgagcc agtcggggaa ctcgttctcc acgaactcga agatgtcgtg cgcctcgcag
    27661 agccgctccc tgagcgccgg atccttgccg tccaggatgc ccagcccttt ttccgtgatg
    27721 tcgtgcaggg cggccatgat ctccatgcgc tggcgcatga agcttagcca gccgcctttg
    27781 cggtagcgga agtaatccct ccggggaacg ggggagggga cctgctcgat caaccccgaa
    27841 gaggccagga gccgggtcat ggtcgagacc gacgccttcg aggccctcgc ggcctctccg
    27901 atgtcgttca gggactgttc gggggggtcg cagatcagga gtacgcccag gatgcggcct
    27961 gccatccggg gcatgcccat ctgctcgaaa aaaagcccga tatcctcgat aaagtgaagt
    28021 tcacgaattt ccgttccaac catgggaagt cccccgtggg atcggagtat ggcattcccg
    28081 aaattccatg tcaaggtttt gttcttaacg ttcagtattg actaaacgtt aagaacaaat
    28141 tagtattcct gggagcgccg ggaaagacgg cagtgcgccg cacccggccc atccgaatac
    28201 ccaggagaga aaaatgccgg attcgttcga tcccgcggac ctgcggggcc tgtcctccga
    28261 ggaagttctc gagcgcctgt cctcggaggg gtacaacgag cttccccagg gcaagaaacg
    28321 gggtcttttc catatagtgt tcgaggtatt tcgggaaccc atgttcctgc tcctggtcgc
    28381 ctgcgggctt ttgtacctga tactcgggga tcccgaggaa gctctaatgc tcctgggatt
    28441 cgtggccgtc atcatcggga taacggtcta ccaggaaggc aaaaccgaaa aggccctgga
    28501 cgcgctgcgg gacctctcca gtccgagagc cctggtggtc cgggacgggg aacagcgccg
    28561 gatagccggc aaggaggtcg tgcgggacga catcgtcctg ttggccgagg gcgaccgtgt
    28621 accggcggac gcgctggtgc tgcaccaggt gaacctgtcc gcggacgagt ccctgctgac
    28681 cggggaatcg gtgcccgtgc gtaaggccgc gtgggacggg aaagccgatc ccgggcggcc
    28741 cggcggcgac gaccagccca tggtgtactc gggcagcatg gtcgtacggg gtcaagccgt
    28801 cgtgcgggta acctccacgg gaatacagac cgagatgggc aagatcggga aggcgctcca
    28861 gaccatagag accgggcaga cacggctcca ggccgaaacc ggccgaatcg tcaagtcgtt
    28921 cgcgctggcc ggtttgttcc tgtgcgtact ggtggtgctc gtcttcggat tcacccgggg
    28981 caactggctc cggggcgtgt tggcgggtct gtccctggcc atggcgaccc ttcccgagga
    29041 gttccccgtc gtgttgaccg tcttcctggc cctgggcgcc tggcgcatct ccaagcggtc
    29101 ggtgctgacc cggcgcatgc cggccgtcga gaccctgggc tccgccaccg tgctctgcac
    29161 ggacaagacg ggcaccctca cgatgaatcg catgacggtg gccgagctct gcgtggggac
    29221 ccgggtctcg aaggtggggc aggcggacaa cctgctcccc gaggagtatc acgcgctcgt
    29281 ggagcacacc ctgctggcca gccccaagga tccgttcgat cccatggaga aggcgatgcg
    29341 ggaactcgga gtacgaacct taatgaacac cgagcacctc cgggacgact ggaagctgtt
    29401 gcgcgaatac cccctctcgg aaaagctcct ggccatgagc cgcgtctggg aatccaccgt
    29461 cgacggcggt tttgtcgtgg ccgcgaaggg tgccccggaa gccgtatccg acctctgtca
    29521 ccttgatgcg gcggccgcct ccgaagtgcg cgcctgcgtg gaccgaatgg ccgccgacgg
    29581 gctccgggtc atcgccgtgg cctcgtcccg gatccccgct
//
LOCUS       DAOTEI010000022        29300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1051_length_29300_cov_5.595657, whole genome shotgun sequence.
ACCESSION   DAOTEI010000022 DAOTEI010000000
VERSION     DAOTEI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 29300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29300
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1051_length_29300_cov_5.595657"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="P5313_03600"
     CDS             <1..462
                     /locus_tag="P5313_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRY79483.1"
                     /translation="PRRLGATVTAHHLSFTTDDLAGRALNPHLYCKPVLQDAAHRAAL
                     RKAAAACERVFFGSDSAPHPRDRKESGSIPPGIYAAPVALPLLAAAFEEEDALDRLED
                     FASARGASFYGLPPNRSRVRLVRETWTVPDEVDGAVPAAAGSALAWTAERV"
     gene            581..964
                     /locus_tag="P5313_03605"
     CDS             581..964
                     /locus_tag="P5313_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79484.1"
                     /translation="MDRPRILVVEDEPLAAMEMQETLEDAGYDVPEIIASGDAVLPAV
                     LRWKPSLVIMDIRLASFTDGVDAAERMRVLSDAPVIYVTAYPGRGSQDRAARTRPYAY
                     LLKPLAPGLLRKTVERALSGQAAVP"
     gene            996..1349
                     /locus_tag="P5313_03610"
     CDS             996..1349
                     /locus_tag="P5313_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79485.1"
                     /translation="MMTRLTEAQCRSALSRGEFEADVLGSAASVAVVLTQSWCPQWVW
                     MRSYLEGLPDSPEYRVYYLEYDREPFYEEFLAFKEDTLGNREIPYVRYYRDGRLNAQT
                     NFIAKDGFLKRLAGF"
     gene            complement(1404..2051)
                     /locus_tag="P5313_03615"
     CDS             complement(1404..2051)
                     /locus_tag="P5313_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79486.1"
                     /translation="MKRMFLAAAMLLVSVCGFALDPEYLNPRIGVILINNVVTGQVGA
                     PDALVNTLGASLSFPFKKNPRFSFDPGLDLYWTRYEWGADLRAHPTELETADAGFVVG
                     AILDAPVLWRWEIADRFDAHLGAGGSLVIRATFRTADSAAEDVRSIGAWFYQGLRWFY
                     PNAQVRLSYRLQERFTFEFILRGFLPLFNALDPDAPSFFDHAMLNAAMGMRIKFR"
     gene            complement(2079..3185)
                     /locus_tag="P5313_03620"
     CDS             complement(2079..3185)
                     /locus_tag="P5313_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79487.1"
                     /translation="MSRAGAFREVDEARLRCRGGIAAALCLAALCLAAGCARRADPEP
                     VEEFPESFAQGAVLSLSGGRLAALPAGAVSVPPGLPSGVAPAVAALAAVDGRTAALAL
                     NRTGLVLLRIDPERRRYRIDPLESGEEFDGRTVGGLFLREGTLYCTLYRDPHFETRPT
                     RDPPSVLVAADPGNPASPARPFDLGLGAEGSGLFALFPQPDGSWALQFRRAVEDGFES
                     TFRRYDPASGSSLALSRSVFEGLLAPRLLASAPDTLRRAARALAPEGAPAVVSASLAD
                     GSRGAYLFGDGPAEDTVELRGAVSASGAALVSWNAVAAVAREGSETALRLPVPIPGAV
                     YRDAVPLEGAILAVWEVGVFPNIEESGIVLLPVP"
     gene            complement(3348..4814)
                     /gene="proS"
                     /locus_tag="P5313_03625"
     CDS             complement(3348..4814)
                     /gene="proS"
                     /locus_tag="P5313_03625"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRY79488.1"
                     /translation="MAEKITSRAVDYSQWYIDIVLNAKLADYSPVKGCMVVRPRGYAI
                     WERIQTEYDRRFKETGHQNAYFPLLIPMSFLKKEAEHVEGFAPELAVVTHGGGEELEE
                     PLVIRPTSETIIWSKYKDWIQSWRDLPLLYNQWCSVLRWEKRTRLFLRTAEFLWQEGH
                     TAHETEKDAVEETMRMLDVYREVAERHLCLPVIAGRKSDAEKFAGAVATYCIEAMMQD
                     RKALQAGTSHFLGQNFGKAFEVQFQTRDGGLDYAWATSWGLSTRLIGAIIMTHSDDKG
                     LVLPPSLAQEQAVIVPIYKTDSKAAVLDYASKMETSLKDAGVRVVTDTDDTSSPGWKF
                     AEWEMRGVPVRIEAGPRDMEAGKAVLVRRDTGEKLVVAAAEVAVKVRELLDAIQKNLY
                     DRAKAFRLANTRDVADYEEMKAFFGKAGEENEGTATARGGFARALWCGSRECEAKLKE
                     ETKVTLRCMPLDDQDHIEGKCALCGSPARHRAIFARNY"
     gene            complement(5051..5743)
                     /locus_tag="P5313_03630"
     CDS             complement(5051..5743)
                     /locus_tag="P5313_03630"
                     /inference="COORDINATES: protein motif:HMM:NF021529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HRY79489.1"
                     /translation="MKRMQALCLALVLLAAPLWAGDVAFFVNLGFSPDSAYYMFGQYG
                     LDTRTGTPYAETFIVDTKANDFVRKGTARMTYAVRPEPGQDPAAAFYALLADQGAARL
                     KEYRIDPLRQGRIVYLLVNGQTPSDALSFRDYKTEAQWEVLLRPVTTEANGEVTSSFG
                     ISASITSKGGTVKRFEAGNPGIKRKGVRGYAIRRILVTPDEKTVVFIVEKRVAEKDGD
                     SVRYMVETVRIP"
     gene            5831..6658
                     /locus_tag="P5313_03635"
     CDS             5831..6658
                     /locus_tag="P5313_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79490.1"
                     /translation="MTISSRTRTYRACWILALVFAAVSATAFLRLGPDALTGAGRLPG
                     YPAHGRGMEILARYPGALPVAAAVLCSAYGIVVLFLLSFRYSRTVSMELYFFAFWALA
                     LAAEALRVESLRGALLGASFPERSIVTRLLLSMRFLGIFSLFAGSLYSVGYSEDKQSV
                     PIGAVLLASLALGTGLPINTGIFGADLLQRVGYTQLYGFLYAACLLGASANYALAARL
                     TGEKGYNLAALGNALIFVGGLALRASRSPGTVILGTLGLAAGTVLFLRKIHAYYLWL"
     gene            complement(6700..7326)
                     /locus_tag="P5313_03640"
     CDS             complement(6700..7326)
                     /locus_tag="P5313_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRY79491.1"
                     /translation="MEKSLVIAAGRTGLARFSGKIETEIVRKSIHILVGIVPTLAAVN
                     LQVTSTLLGFGILFYTYCELLRLRGYEVALVSRVTALAARRRDAGRFVLGPVTLGMGA
                     LFSLLVFPEPAASIAIYALAFGDGLSSLMGRLFGTVRIPYTGGKSLEGSLTCFLAVWV
                     SAYGRTGDPAGALAVAGVAMVAEALPTRDLDNLILPLVVGLTARLIFP"
     gene            7570..7641
                     /locus_tag="P5313_03645"
     tRNA            7570..7641
                     /locus_tag="P5313_03645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7602..7604,aa:Val,seq:cac)
     gene            complement(7689..10943)
                     /locus_tag="P5313_03650"
     CDS             complement(7689..10943)
                     /locus_tag="P5313_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY79492.1"
                     /translation="MPNLEDIDRFKQDIRALGRESELLEQWGEAYEDVPPPEQGLPQD
                     LSELMGPAPGGEAAPASPEGDLASFLDNLALGAEPGGEESESPLAAGLEAPEAFSPRE
                     TFGEEGGEGAASVEALGKSIPDFGGLEDLTGTEGLADRPREAPEVPVPEDEGGIPEGL
                     LGGLTEDLETPVPEFPETDFETAPEAPEETPGIVDDLSAFELSEPEAAAPEPAAEEPA
                     GFEEIPLAPEEVSLFGEEEAPESSEPAAPGLPDFGLPEEPLPDLSEAGASAAGAEDFE
                     GFDVSIPELGDAGGESPEPLRAAGAAPEEPAADFGELSPGPGPAPEEPSDSFETFRLD
                     EAELSSAPPPAAGAGGLNLDEELASLENDLTPSENFNLESAWGAADFSIPGYETPAPA
                     AKKPAAEKAKGGSGPGRPDRESRAFREPPREGEDESVPEVTLTEDQVDALQDTLLSYP
                     LNLRLAIEEALAEEKGTEAQRSRLVLLLAKGATPGEAASQASRILKRPISVPKDFEKR
                     TGAELEAEKGSLRYILIHTVAPIAAMVLAVVLGLGALGFLGYRFVYRPIRAELLYREG
                     YEEIRQDRFPEAETVFQKADRVWVHKRWYYRYAEAFAAKNQFVSAAGKYEALLKRWPN
                     DRKGVLDWAALEFRSRLDYAKAARLLKGFIEARNYFDKDALMLLGDVYLEWAESEGDR
                     GERLFEDARLQYTTLVERYGAEDPYMERMLRWFLRTDRTLGRDNYEDIRKLKDRFLGD
                     PKSRISSETLADLGGYLLDRGEVEDVSRILLRAVDQDPSVPEAHYELSRYFRRIGNAP
                     EERRALDNAIRILEDVPNPTNRRLGMLVEALNRAGEGYVELREYVTAEDLFTRAVRRY
                     ERALSLGQLRRSPVYGKAYANLADILFFQRDDLDGAAVLYGKSHDHGYVTPDTLYKLG
                     YVHYRRERWEQALENFYRAGLDSEESPYLLYSTANALFQREDFFAAQGYYARLVDRLE
                     FELDQLPLVEPQARPSHGEIVDLLMRTTNNLGVSLYRIGNRMGDPRRRASAMAQLMES
                     SRYYDSLERDPATMIRPETRNLGYLNLDFVLHPQRGIDLSIYPDLPKRMKFPRD"
     gene            11042..11743
                     /locus_tag="P5313_03655"
     CDS             11042..11743
                     /locus_tag="P5313_03655"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79493.1"
                     /translation="MKHRTAIAALLLALAAQAAGAFQVESLSLHLHLAGLKKAAPPQV
                     VEGYLILTAEGSFRHVGASFAHESFRTVHSFQRNAHGLFILAVPVPYEVREPLKYRLV
                     VDGIWGRDAVNPEFERDPRTGAVFSLAAVPFLSSARPGAWNLVAEDGRTARFRYESDP
                     GLSVAVIGDFNAWDPFVHELKETRPGVYELALPLPSGEHRYAFLVRGDRVPDPLNPAR
                     LYDSSGRTVSVIRIR"
     gene            complement(11800..12669)
                     /locus_tag="P5313_03660"
     CDS             complement(11800..12669)
                     /locus_tag="P5313_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY79494.1"
                     /translation="MPPEPPRADPSGNYRAAIEAFVVGYHKSVVLTAVTANSLEILDD
                     SMGRIGAALASIVSAFEEIRATSGSTAGNSARIDSMMAEILRKNEGMNGDIEARVGEI
                     VQASRDAGTLAGLFQNIKDKTSAVAGITGAIQDVSDRTGILAINASIEAARAGAVGRG
                     FRIIANEVRTLAQQTGDFAKQIDQNVGDFRKAVDAVSERMDGFLALLGRFHESFTGIL
                     EAFRENSRSVTQAGEFLSEISSALKEENLALTDGLESLEKISQSAQDTHAVFGALQSS
                     YRYLDTLLSRADR"
     gene            complement(12671..13447)
                     /locus_tag="P5313_03665"
     CDS             complement(12671..13447)
                     /locus_tag="P5313_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY79495.1"
                     /translation="MNDEADLTSRVLYEDGDHSFIWLGADARSGKGAVQTMQYLILDK
                     GRGWLLDPGGVHLFARVVAAVSRHVPMDRIDGIFFSHQDPDVSSGIALWLGVTPARIY
                     ISGLWTRFLPHFGIVDPGRIQPIEEAGKGVRLGSGAELSFVPTHYMHSPGAFSLFDNQ
                     ARILFSGDVGGGFFPEGGETAFVDDFSAHLPVIQGFHERLIASNSVARRWCDMVSRLK
                     PEMIAPQHGAIYRGASVQGFLAWLSALKCGTDLLDRFYGA"
     gene            13501..14178
                     /locus_tag="P5313_03670"
     CDS             13501..14178
                     /locus_tag="P5313_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79496.1"
                     /translation="MEAQSRGIGNRLIRALGVLTESAPGRGALAVSAAASFLSARSPG
                     AALVFVLLPAYVVLTRGDFSTAAGPEAGAAGRSPAGEIPSPSEIPLRLAAAALAAAAG
                     ILALGIGGTIAQRFRTQVFTLPWGLGAACAAASLILAAVHLPFLAAFGRRAGRIAAGT
                     VFAAAGIAAALSSFPVGSLYAAFLWLLYSGSAPALYLPLVLGAAALAGLSAAASRAIL
                     RARAAGR"
     gene            14175..15218
                     /locus_tag="P5313_03675"
     CDS             14175..15218
                     /locus_tag="P5313_03675"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY79497.1"
                     /translation="MKSPFRRDGLGRAVVGALVLPGAGALLALAACRWGPAFPAWAAL
                     GAAAAGAFLWWNLKPLPVRTRNPAPSSPDTGSLMHPDAAPLAQLKGHRDLLFCVHGFP
                     ATPAEFRPFLPEADVLGYDLAAPLLPGCGTRPRDLAGQDYDSFLHHVRSEYLRLRPRY
                     ERVFLVGQSLGGTLALALAEEYCAVPALAPSALAAIGAPVVLNSLVRHGMVRHPLIYA
                     ARFLGLFTACLGARVPDPDREGEDGDERWLGYEGIYPRIVHTMQIRFPEVERKLRRVT
                     CPVLVCHARGDRMTDYRNAAIIAGGVGSDRIETYTANMDRFRHIRHSLLLYDSQRDRV
                     RERILRFFGEFRS"
     gene            15242..17161
                     /locus_tag="P5313_03680"
     CDS             15242..17161
                     /locus_tag="P5313_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRY79498.1"
                     /translation="MIEVRDGRFRDAEGRTLILRGANLGGSSKYPARPDGRTHVREGF
                     YEGRDVSFVGRPFPRAEAGEHLDRLKLWGLDFLRLLVPWEAVEHAGPGLYDEEYLDYL
                     EGVAAAAEERGIRLFVDPHQDCWSRWTGGDGAPSWTLEEAGFELRNLHASGAAFLHQE
                     HGDPCPRMVWAANYARLGCATMFTLFFGGNHAAPELRVEGESFQERLQGHYIRAMATV
                     ARRLGRFPNVVGFDSMNEPSAGFLGLSDLRRLERALTRTGPMPSPWEAIRAGDGRAVE
                     VDRYGITPRGQSVVGRAILGREGVRAWREGVECLWKRAGVWDEARGKPVLLRPDAFSR
                     TRDGRPLDFGEDCLKPFARRYIREVRAAAEGGRRFLLFLETVPMDEPPAWSPADDPAA
                     VNAEHWYDALTLTLKRWFGFLGYDGEDGRIVLGRKALRSYFREALERVVRRGRERMGG
                     IPSLLGEFGLPFDLDGGRAYRTGDFRKHEEALAAYHDALDAAGLSSTIWNYTADNTNE
                     RGDGWNGEDLSIYSSDQARLPLPAGADPREAGGRAIRGFCRPYPRATAGDPQSFRFDA
                     RTGDLEYRFLPDPAVAAPTELYLPPVQYPEGCRVEAEGGTFEPDLPGRVLKVRPHPGS
                     AEVTVRVRRADRKPR"
     gene            complement(17188..18639)
                     /locus_tag="P5313_03685"
     CDS             complement(17188..18639)
                     /locus_tag="P5313_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY79499.1"
                     /translation="MRAFRYGFLILIAALAAVPGAGQGLAITQIDTERLLFGQSVRLY
                     LSAPGAAGTESPDVEVWESGEGGEWAPRPVTAVSRGINDAEGIAFCFLLDNSGSMWTG
                     LDGREGAPTEDQRMTHAKAAARAFLAEASRQGRGKDRIGLGVFNTRYWKAADPVGDFE
                     RISRGLDEIRKPATEDAYTELYLSLDRALADFAGVPGRRALVVLSDGENFPYYIRTGK
                     PNPETGTRTTGPEDVVERAAREGITVYVVRFGNEKDPSLGDAAVRTGGKVFDASNRDE
                     LAAVYSTIRADVLAEIAVDYRAGMAPGSRRNVRAVLRDREGREFSSERYYYAGTVLGW
                     SRDLPGWYYLAFLLVPALLWLALLVFKLERETTEAGLRLLYGPSGGGTRVFTLNSPQT
                     VIGSSDAADFTIGGNPSLKERHATILFDERQNAYTVVGDGNLTVNNREVERKRLEPGD
                     VINMAGTVVVFDDALVKKTPKGRLPQPSRRKSQ"
     gene            complement(18662..19555)
                     /locus_tag="P5313_03690"
     CDS             complement(18662..19555)
                     /locus_tag="P5313_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY79500.1"
                     /translation="MSLTLKRAVMAVLGILGALMLWPILLMVQRAQAAFPSYLVFTLS
                     QGVCLGLVFGALFGSFEGIVVSSRSKAFRGLGFGALFGAAAGAAGVLAGQLFLFLAGG
                     ALFRSNSSRLGPGLAAANGMAWAIAGTFLAMTEGLRARSGRKLLVGLLGGLAGGLIGG
                     AILSALQVLAPGGKWSLLAGLAAFGFLLTLFYSLFENRFQAGTLKLLNGPLKGKEYPV
                     VSRKLTVGTDASCDIVLAGYPGVRPLHATVHLEKGRVYIRPAGPDAGVLVNDGPAGDA
                     ALRPEDVLAAGKAKFLYGYFA"
     gene            complement(19559..21436)
                     /locus_tag="P5313_03695"
     CDS             complement(19559..21436)
                     /locus_tag="P5313_03695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY79501.1"
                     /translation="MAVFPELIGKYPIESLVAKGGMGAVFKALHPTLKRHVIIKKLTL
                     RGSATITERFKREARILMDFKNDHIVRVFDHFKEGSSHYIVLEYVDGMSLDQLIKRQR
                     SLSSELALVIFLDACRALQYAHGMGVIHRDIKPGNILISRKGEVKLADFGIAASEEED
                     DSGLTKEGMTLGTPSYMPPEQIENSKNVDRRADIYAMGIMLYEMVTGKKPYPGNFAPE
                     TVVLIQKGRYRAAGKVNPRVLPFVDKLIKKLIKPDPRKRFQTMDAVIKTVEKFLSRYS
                     ADSLRSVLSDLVSGKCQDEPRYRPAPRKRFLLAAILVLLAGIGAGGWYAWTEGLVHRY
                     LMPDRWGELRLVVRVPKSQAEAADIFLRARIFHNDGKEIPEVEDVRIVLRPAETEGAD
                     PFHTFVSNRLFLAPGPYRVKLTADQDLHWESFHLLPYSMAAPGEDGVRVLQFRLEDRP
                     PRPLAVLTEARDALTGTVLGEAVFSAYLRGEWKPLDKLESGDLTSGAVWKLRAEAEGY
                     FPEVFSLRIANAQERLRLEARLAPRPGSLVVETPAGSYRLTLNGLGTAASGGPEMLPL
                     RLGTVKEGRVEWELPASAYELGVARGGASGRASFRLEPGRTVRIRVSDEGGTLRFRVE
                     E"
     gene            complement(21446..22513)
                     /locus_tag="P5313_03700"
     CDS             complement(21446..22513)
                     /locus_tag="P5313_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79502.1"
                     /translation="MSAAEAVRAAFLRRGLPDREEYLAKVEAASDALDAEGGPGRPRD
                     SRGLPGGLVRLAGDAPTILLPDLHARTDFLASVLFQPLPEGQTVLDALGEGALQILCL
                     GDGFHAESRALDRWKRAYQEFAGGYAKHKAMDQEMRESLSLMEMVLVCKAAFPGRFHF
                     LKGNHENVLNEEGNGNHPFRKFALEGMMVTSWLERFYGRDFLERYAGFERRLPLFATG
                     PRFLASHAEPQRAYSEMELIDARLDPGTVLGLTWTDNGAADPRAVPVLLDRFLPGIPG
                     ARYFTGHRAIRGVYRELSGGRHLQIHNPDRFVVAWVMPGRDFDPELDIGEIRDITADL
                     RKVHAADLKRPRTADPRKSER"
     gene            complement(22530..23054)
                     /locus_tag="P5313_03705"
     CDS             complement(22530..23054)
                     /locus_tag="P5313_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79503.1"
                     /translation="MSTFETLEPIIQDHIRQIAKTSGLPAGPDTYEMMADAWLEKKTC
                     FEEALAGNDLEEVSFFGRAEARGALVLTYSGSLLNIGPQVEGLRRCEYTSIGMRTDVP
                     ASAVEEASVLEADLEADEPARFEKGPIKSSSPVFKIAVAKAKLEAEEEEALLTQVSQG
                     LAEDFVEVNKTVIE"
     gene            23239..24135
                     /locus_tag="P5313_03710"
     CDS             23239..24135
                     /locus_tag="P5313_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRY79504.1"
                     /translation="MKEVKFEDLPEAIRKQLLSLIQDSGLDGDEETRKRLAVIWDEKY
                     RLFTGQVGALGMELVPELGKEDTRGAVLLTYSGSLISLGPLRGRSRWLEYASIKLRTD
                     VPELVRGDKASLAGPIRLDQAAVFEGCPVKRSSALFRIAVCPEGLSADDQERRIREAT
                     VYLTNGFVKLNRTHSLEPVPEVDQFTAKAIIGYIAKKNGVTQLAARGLIEDYLSMVET
                     GMLLGERVSVGKLGSASLRYQAARKARLTRNLRTGEELLIPAKPACLAPRFTFSQAVK
                     DKCGSVDPTLFGAEEGGEDEED"
     gene            24228..25931
                     /locus_tag="P5313_03715"
     CDS             24228..25931
                     /locus_tag="P5313_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79505.1"
                     /translation="MDPTRRDPEKSYLFAALGAFDAAVLSALAWAAITVLTKYQIGYM
                     AVAVGALVGISVRWFGQGSGKAFRILSVGFALFGCVFGNYLSQVGFYLAESGAGLLDT
                     LLSLRPAQVPAILAESFSPIDLLFYALAVFQAWKLSVAPAAPEAETAAAAEGSASDAE
                     PRPLLPPYPPRKALAAALPFLAILAIPVYVAARSDGTARDVYDSGSPKYEGPTSWNEP
                     HGVWKYWDESGTLLAEIEFHRGVPEGRSVHYAEDGSMSERKTWKAGFLHGPYEQFYPD
                     GTLRAKGAYEYDRKTGPWEEYHPNGRLSSSGTMLLDLQHGTWEIRSDSGALRARTAFD
                     QGEPSGVWEGYSEEGILTLRRDFAGGRERILYQRDGDRETVVDGEGRYVERYEDGTRA
                     VEGPVAGGIKTGTWRDWYPGGKPRRVFEYSDGREYLKDYYLETGERAVRDGDGILSTG
                     EEGGRIEARYENGLLTGELKTYYPDGSPLGLSTYSAGLLDGPQRTWAPGGILLVEGEC
                     RGGLRTGVWTWYEEDGSVSSTVTFAEGKKQGVQTFFEDGVLVKEEVYRDGAYVETRLR
                     R"
     gene            complement(26001..26519)
                     /locus_tag="P5313_03720"
     CDS             complement(26001..26519)
                     /locus_tag="P5313_03720"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRY79506.1"
                     /translation="MRYAEIPVSDAYELQNAGGLVLVCTRGPDGRYDVAPVAWCCPLD
                     YSPASKLILVLDTGHRTYADLSDSGEFAVALPTKGQMDLVARAGSMSGHDADKYAELG
                     IPAFPAREVDARIPEGVAGWLECRLSRIVVEGTSGVVMGEVLRAEAVPDAWKLRVHYV
                     REGLRFTPGESF"
     gene            complement(26553..27791)
                     /locus_tag="P5313_03725"
     CDS             complement(26553..27791)
                     /locus_tag="P5313_03725"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79507.1"
                     /translation="MLRSAIHSFSIYRGLPASIYTLFFATVVNGIGVFVFPFLTLFLT
                     KKMGMGERAAGDFLLLVSLAYIPGTLAGGRLADRFGRKRVMLVSQALCGLLFIPCGFL
                     GAHALVPWLILVSIFFDGVTDPARSAMSTDLTTPENRQAAFSLLYLGHNLGFAFGPLI
                     AGFLFENAMSWLFWGNGIAVLASLALVILFIPESKPSDHQIRESLAGNSTERAHEGNL
                     LSALRSRYFLVIFTALSAGYGFVYAQHRFALPLQLEALFAEKGAPLYGTLMTLNAVLV
                     ILFNAPLVAALKRFRPVVNVAVAGLLYMAGFGMIAFIRSPALFLVSTTIWTFGEIVNA
                     TNEQVYVANHTPMSHRGRFNAILPLIGGMGFALSSPVTGRIIEGSGLGPVWILLGAVA
                     LAAAGGFLLVDRSERRAATR"
     gene            complement(27882..28262)
                     /locus_tag="P5313_03730"
     CDS             complement(27882..28262)
                     /locus_tag="P5313_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRY79508.1"
                     /translation="MPRKAFTAPGSVAVGPYSHAVEAGGFVYLSGQTPAIPGTSRLAE
                     GGIEAQTAQCFENLKNVLAAAGLTLDDVVKVNVFLTDMKNFQAMNEVYKSKFEAPYPA
                     RSTVGVRELPLGALVEIEMIARRN"
     gene            complement(28323..29057)
                     /locus_tag="P5313_03735"
     CDS             complement(28323..29057)
                     /locus_tag="P5313_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRY79509.1"
                     /translation="MLLSDTGLSDTFDERVVTGDWGIGMLKAVLFDLDGTIIDSEPLH
                     TEAVLRLLRDRSVDLKASDLDRFIGISSSVMWREMRERFGFAESVEALKELQHRANVE
                     MLEGSESILIPGVLPLLRDLRDRGIRTAVASSSTRDYIETVLSKYELDSLFDVVVSGE
                     EVPRGKPRPDVFLRAAELLGVEPGSCVVIEDSDNGLTAAREAGIRSIGFRNPNSGRQA
                     LSLADRIVDDIREVSVALLQRLVDSE"
BASE COUNT         4545 a        10155 c         9921 g         4679 t
ORIGIN      
        1 ccccggcgcc tgggcgccac cgtgacggcc caccacctgt ccttcaccac cgacgacctg
       61 gcggggcggg cgctgaaccc ccacctctac tgcaaacccg tcctgcagga cgccgcccac
      121 cgggccgccc ttcggaaggc cgcggccgcc tgcgagcggg tcttcttcgg ctccgactcg
      181 gccccccatc cccgggaccg caaggagtcc gggtcgatcc ccccggggat ctacgccgcg
      241 cccgtcgccc tccccctcct ggccgcagcc ttcgaggagg aagacgcctt ggaccgcctg
      301 gaggacttcg cctccgcccg gggcgcctcg ttctacggac ttccaccgaa ccggagccgg
      361 gttcggctgg tccgggaaac ctggacggtc ccggacgagg tggacggggc cgtcccggcc
      421 gccgctggat ccgcccttgc ctggacggcc gaaagggtgt aaccttacgg ggttgccgga
      481 ccttgtatgt acgcggccgc cgttcgagcc ccgcgggccc ggcccgtcga agcgaaaaac
      541 agaatttggg aatgttaacc ttttactcac cgagggatgc atggacagac cccgaattct
      601 cgtcgtcgag gacgagcctt tggccgccat ggagatgcag gagaccctgg aggacgcggg
      661 ctacgacgtg cccgagatca tcgcctccgg ggacgccgtc cttccggccg tcctgcgatg
      721 gaaacccagc ctggtgatca tggacatccg cctggccagc ttcaccgacg gagtggacgc
      781 ggcggagcgc atgcgggtcc tctccgacgc cccggtcatc tacgtgaccg cctacccggg
      841 ccggggatcc caggaccggg cggcccgtac ccggccctac gcctacctgc tcaaacccct
      901 ggccccgggc ctgctccgga agaccgtgga gcgggccctg tccggccagg ccgccgtgcc
      961 ttgaaggcgg gccgccttcc gggtacactc cggccatgat gacccgcctt acggaagcgc
     1021 agtgcaggag cgccctgtcc cggggggagt tcgaggcgga tgtcctcgga tccgcggcct
     1081 ccgtggccgt cgtcctgacc cagtcctggt gcccccagtg ggtctggatg cgttcctacc
     1141 tggaagggct tccggatagc ccggagtacc gcgtctacta cctggaatac gaccgggagc
     1201 ccttctacga ggaattcctg gcgttcaagg aggataccct gggcaaccgg gagatcccct
     1261 acgtccggta ctaccgggac ggccgcctga atgcccagac caatttcata gccaaggacg
     1321 gtttcctgaa acgcctggcg gggttttgaa ccgcatcgag tcttgcgaca acctgtcggg
     1381 acgggccgga accggaggat ttcctaccgg aatttgatcc tcatgcccat ggccgcgttc
     1441 agcatcgcat ggtcgaagaa ggacggggcg tcggggtcca gggcgttgaa gaggggcagg
     1501 aagccgcgga ggatgaactc gaaggtgaac cgctcctgaa ggcggtacga caggcgcacc
     1561 tgggcgttgg gatagaacca tcgcaggccc tgatagaacc acgccccgat cgaccgcacg
     1621 tcctcggcgg cggaatccgc ggtccggaag gtcgcccgga tgaccaggga tcccccggcc
     1681 ccgagatggg cgtcgaaacg gtcggcgatc tcccagcgcc agaggaccgg ggcgtccagg
     1741 atggccccca cgacgaagcc cgcgtccgcg gtctccagct ccgtaggatg ggcccgtagg
     1801 tcggcgcccc actcgtaccg ggtccagtag agatccaggc cggggtcgaa ggagaagcgg
     1861 ggattcttct tgaacggaaa gcttagggag gcgccgaggg tgttcaccag ggcgtccggg
     1921 gccccgacct gcccggttac gacgttgttg atgaggatga ccccgatccg gggattcagg
     1981 tactcgggat ccagggcgaa tccgcagacc gagaccagga gcatggccgc cgccaggaac
     2041 atccttttca tggggccctc caccaggaac tataccactc acggcaccgg aagcaagaca
     2101 atcccggatt cttcgatgtt ggggaagacc ccgacctccc agaccgccag gatcgccccc
     2161 tccaggggaa ccgcgtcgcg gtacaccgct cccggtatcg gtacgggcag gcgcagtgcg
     2221 gtctccgagc cctcccgggc taccgccgcc acggcgttcc aggagaccag ggccgccccg
     2281 gaggctgata cggccccgcg aagctccacc gtgtcctccg ccggaccgtc gccgaagagg
     2341 taggcccccc ggctgccgtc cgccagggac gccgagacga cggcgggcgc cccctccggg
     2401 gccagggccc gggcggccct gcggagggtg tccggggccg aggccaggag gcggggggcc
     2461 agaagcccct cgaacaccga gcgggaaaga gccagggaag atccggacgc ggggtcgtag
     2521 cggcggaagg tgctctcgaa gccgtcctcc acggccctcc ggaactggag agcccaggag
     2581 ccgtccggct gggggaagag ggcgaagagt ccggagccct ccgcgcccag gcccaggtcg
     2641 aaggggcgtg cgggcgaggc cggatttccc ggatccgccg cgaccagtac ggagggggga
     2701 tcccgggtgg gccgggtttc gaagtggggg tcccggtaca gggtgcagta cagggttccc
     2761 tcccggagga acaggccccc cacggtccgg ccgtcgaact cctccccgga ctccaggggg
     2821 tcgattctgt aacgccggcg ctcgggatcg atccggagaa ggaccagtcc ggtccggttc
     2881 agggcgaggg ccgcggtccg gccgtcgacg gccgcgaggg ccgcgaccgc cggagccaca
     2941 ccggagggca ggcccggagg gacggacacc gcgccggccg ggagggcggc caagcgtccc
     3001 ccggagaggc tgaggacggc gccctgggcg aaggattccg gaaattcctc gacgggctcg
     3061 gggtcggcgc ggcgggcgca gcccgcggcc aggcagagcg cggccaggca gagcgcggcg
     3121 gcgatgccgc cgcggcaacg aagcctcgct tcgtcgacct cccggaaagc ccccgcgcgg
     3181 ctcatcggta atccctcgcg aacatggccc gatgccgcgc ggcggcgatg ccgccgcggt
     3241 tcatcagtag ttcctcgcga agatcgcccg gtggtgcgcg gaggcggcgc ctccgcggcg
     3301 acgaagcttc gcttcgtcga tcgcccggaa ctcccccgcg cgaatcatca gtagttcctc
     3361 gcgaagatcg cccggtggcg cgcgggggag ccgcagaggg cgcacttccc ctcgatatgg
     3421 tcctggtcgt ccaggggcat gcagcgcagg gtgaccttgg tctcctcctt gagcttcgcc
     3481 tcgcactccc ggcttccgca ccagagggcc cgggcgaagc cgccccgggc cgtggcggtg
     3541 ccctcgttct cctcgccggc ctttccgaag aaggccttca tctcctcgta gtccgcgacg
     3601 tcccgggtgt tcgccaggcg gaaggccttg gcgcggtcgt agaggttctt ctggatcgcg
     3661 tccaggagct cccggacctt gacggcgacc tcggccgcgg cgacgacgag cttctccccc
     3721 gtgtcccggc gcaccaggac cgccttgccg gcctccatgt cccggggacc cgcctcgatc
     3781 cgcacgggca ccccccgcat ctcccactcc gcgaacttcc agcccgggga gctcgtgtcg
     3841 tccgtgtccg tgacgacccg gacccccgcg tccttcaggg aggtttccat cttcgaggcg
     3901 tagtcgagga ccgcggcctt gctgtcggtc ttgtagatcg ggacgatgac cgcctgctcc
     3961 tgcgccaggg agggcgggag gaccaggccc ttgtcgtccg agtgggtcat gatgatggcg
     4021 ccgatcaggc gggtcgagag cccccagctg gtggcccagg cgtagtcgag gcccccgtcc
     4081 cgggtctgga actggacctc gaaggccttc ccgaagttct gccccaggaa gtggctggtc
     4141 cccgcctgga gggccttgcg gtcctgcatc atcgcctcga tgcagtaggt ggccacggcg
     4201 cccgcaaact tctccgcgtc gctcttcctt ccggcgatga ccggcaggca gaggtggcgc
     4261 tcggcgacct cgcggtagac gtccagcatc cgcatggtct cctccaccgc gtccttctcg
     4321 gtctcgtggg ccgtgtggcc ctcctgccag aggaactccg cggtgcggag gaagagacgg
     4381 gtgcgcttct cccagcggag caccgagcac cactggttgt acaggagggg caggtcccgc
     4441 caggactgga tccagtcctt gtacttggac cagatgatgg tctcgctggt gggccggatg
     4501 accaggggct cctcgagctc ctccccgccc ccgtgggtga ccaccgccag ctcgggggcg
     4561 aagccctcca cgtgctccgc ctccttcttg aggaagctca tggggatgag gagggggaag
     4621 taggcgttct ggtgccccgt ctccttgaag cggcggtcgt actcggtctg gatccgttcc
     4681 cagatcgcgt agccccgggg ccggacgacc atgcagccct tcaccggcga gtagtcggcg
     4741 agtttcgcgt tgaggacgat gtcgatgtac cactgggagt agtcgacggc gcggctggtg
     4801 atcttctcgg ccatgatgct tccttgttcg gagaatggat gagggatgat aacggaaatc
     4861 aagggcggga ttcaacctgt ataggaatca gcgagaggcc gcgagaggct gcatccgcgg
     4921 cctctcggcg acgaagcccg cagggcttcg tcgaataccg ggaaactcag ccgaggttcg
     4981 cttcgaccat gttcttgccc ggtcttttct ccgtgccctc cgtgtcctcc gtggtgagtt
     5041 ccggaaaaag ttacgggatc cggacggtct ccaccatgta acggacggag tccccgtcct
     5101 tctcggccac gcgcttttcc acgatgaaga ctacggtctt ctcgtccggg gtcaccagga
     5161 tgcggcggat ggcgtagccc cggaccccct tgcgcttgat gccgggattg cccgcctcga
     5221 agcgcttcac ggtcccgccc ttggaggtga tggacgcgga gatgccgaag gaggaggtca
     5281 cctccccgtt cgcctccgtg gtcaccggtc ggaggaggac ctcccactgg gcctcggtct
     5341 tgtagtcccg gaacgagagg gcgtccgagg gagtctggcc gttcactaaa aggtatacga
     5401 tgcgcccctg ccggaggggg tcgatcctgt attccttgag ccgggcggcg ccctggtcgg
     5461 ccaggagggc gtagaaggcc gcggccgggt cctgtccggg ttccgggcgg accgcgtagg
     5521 tcatccgggc ggttcccttc cggacgaagt cgttggcctt ggtgtcgacg atgaaggttt
     5581 ccgcgtaggg ggttccggtc cgggtatcca ggccgtactg cccgaacatg tagtacgcgg
     5641 aatccgggga gaatccgagg tttacgaaaa acgctacgtc cccggcccac aggggcgcgg
     5701 cgagcaggac aagggccaga cagagcgctt gcatccgttt catccggttt actcctagcg
     5761 tatgtatcgg cacgcggcgc gacgggttca accgcttttt ttcgggtccg cttcgtagta
     5821 agctcgatcc atgacgatat cctcccggac gaggacctac cgcgcctgct ggatcctggc
     5881 cctggtcttc gcggccgtct ccgcgacggc gttcctgcgg ctggggccgg acgccttgac
     5941 cggggcgggc cgccttccgg gctatcccgc ccacggcagg gggatggaga tcctggcgcg
     6001 gtatcccgga gccctgcccg tcgccgccgc cgtcctgtgc tcggcctacg ggatcgtagt
     6061 cctcttcctc ctcagcttcc gctactcccg tacggtctcc atggagctgt atttcttcgc
     6121 cttctgggcc ctggccctgg cggccgaggc cctccgggtc gagtccctcc ggggtgccct
     6181 cctgggcgcg tccttcccgg agcgaagcat cgtgacccgc ctcctcctgt ccatgcggtt
     6241 cctggggata ttctccctgt tcgcgggatc cctgtactcg gtcgggtatt cggaggacaa
     6301 gcaatccgtc ccgatcggag ccgtgctcct ggcctccctg gccctgggca cggggcttcc
     6361 gatcaatacc ggtatcttcg gggccgatct cctgcagagg gtggggtaca cccagctgta
     6421 cggattcctc tacgccgcct gccttctggg cgcctccgcc aattacgccc tggccgcccg
     6481 gcttacgggc gaaaagggct acaatttagc ggccctgggc aacgcgttga tcttcgtcgg
     6541 gggtctggcg ctccgggctt cccgatcccc cggcacggtc atcctgggaa ccctgggact
     6601 ggccgccgga acggtcctct tcctgcggaa aatccacgcc tattacctgt ggctctagga
     6661 gtcccgacgc gtccgtcccg gagtcgtcgc cgtccgcggt tacgggaaga tcagccgggc
     6721 ggtcaggccg accaccaggg gcaggatgag gttgtccagg tcccgggtcg gaagggcttc
     6781 cgcgaccatc gcgacgcccg ccacggccag ggctcccgcg ggatcccccg tccggccgta
     6841 ggcggagacc cagacggcca ggaagcaggt gaggctcccc tccagggatt tcccccccgt
     6901 gtagggtatg cgcaccgtgc cgaacaggcg gcccatgagg ctggaaagac cgtccccgaa
     6961 ggccagggcg tagatcgcga tggaggccgc gggttccggg aagacgagga gagagaacag
     7021 cgcccccatg cccagggtca ccggtcctag gacgaagcgc cccgcatccc gccttcgggc
     7081 cgccagggcg gtcacccggg acaccagggc cacctcgtag ccccggaggc ggagaagttc
     7141 gcagtaggta tagaagagga tgccgaatcc cagcagggtc gaggtaacct ggagattgac
     7201 ggcggccagg gtcgggacga tgcccacgag gatgtggatg ctcttccgta cgatctccgt
     7261 ctcaatcttg ccggagaagc gggcgagtcc ggtcctgccc gcggctatga ccagggattt
     7321 ttccatcggc gaaatccttt cgagatccga taacatcctg tttccgtgcc agatttcgcc
     7381 gtttcccatg ataaaaaaac cgccttgttt gagaatgaat tactcatacc ccgccggttc
     7441 cgggacggcg gtcgtagaaa aaaccataca cctcgaattc acggttcgct tcaccccggg
     7501 cttccgccat ccttgacagc cggccgtacc cccgtatatc atgcggtccg tcggtcgaga
     7561 atccgcgttg gacgattaac tcagcgggag agtgccacct tcacacggtg gaagtcgttg
     7621 gttcaaatcc aacatcgtcc aacgcggatt ttccattttc cccctcaagt cttctttcct
     7681 tcagccgctc aatccctcgg gaacttcatc ctcttcggca ggtccggata gatcgacagg
     7741 tcgatccccc tctggggatg gaggacgaag tccaggttca ggtaccccag gttgcgggtc
     7801 tcgggacgga tcatggtggc gggatcccgc tccagggaat cgtagtaccg ggaggattcc
     7861 atgagctggg ccatggccga ggccctccgg cggggatcgc ccatgcggtt gccgatccgg
     7921 tacagggaca cccccaggtt gttcgtcgtc cgcatgagga ggtccacgat ctccccgtgg
     7981 gagggacggg cctggggctc caccagggga agctggtcca gctcgaactc gagccggtcc
     8041 accagccggg cgtagtaccc ctgggccgcg aagaagtcct cccgctggaa gagggcgttg
     8101 gccgtggaat atagaaggta gggactctcc tccgaatcca ggcccgcccg gtagaagttc
     8161 tccagggcct gctcccagcg ctcccgccgg tagtgcacgt acccgagctt gtacagggtg
     8221 tccggggtca cgtagccgtg gtcgtgggat tttccgtaca ggaccgcggc accgtccagg
     8281 tcgtcccgct ggaagaagag gatgtccgcc aggttggcat aggccttgcc gtagaccggg
     8341 ctcctgcgga gctgccccag gctgagggcc cgctcgtagc gccgtaccgc ccgggtgaag
     8401 aggtcctcgg cggtgacgta ctcccggagc tcgacgtagc cctcccccgc ccggttcagg
     8461 gcctccacca gcatgcccag ccggcggttg gtgggattcg gtacgtcctc caggatgcgg
     8521 atcgcgttgt ccagggccct ccgttcctcc ggggcgttcc cgatgcgtcg gaagtaccgg
     8581 gaaagctcgt aatgggcctc cgggaccgag gggtcctggt ccacggcgcg caggaggatc
     8641 cgggagacgt cctccacctc cccgcggtcc aggaggtagc ctcccaggtc cgccagggtt
     8701 tccgaggaga tcctggactt cgggtccccc aggaaccggt ccttcagctt ccggatgtcc
     8761 tcgtagttat cccggcccag ggtccggtcc gtgcgcagga accaccggag catcctctcc
     8821 atgtaggggt cctcggcgcc gtaccgttcc accagggtgg tatactgcag gcgggcgtcc
     8881 tcgaagagcc gctctccccg gtccccctcg ctctccgccc attccaggta gacgtccccc
     8941 aggagcatga gggcgtcctt gtcgaagtag ttccgggcct cgatgaagcc cttgagcagg
     9001 cgcgccgcct tggcgtagtc cagccgggac cggaactcca gggccgccca gtcgagcacc
     9061 cccttgcggt cgttgggcca gcgcttgagc agggcctcgt acttgcccgc cgcggagacg
     9121 aactgattct tagccgcgaa ggcctccgcg taccggtagt accaccgctt gtggacccag
     9181 accctgtccg ctttctggaa gacggtctcc gcctcgggga agcggtcctg gcggatctcc
     9241 tcgtaccctt cccggtagag cagttccgcc cggatgggcc ggtacacgaa gcggtagccc
     9301 aggaagccca gggctcccag gcccaggacg acggccagga ccatggccgc tatgggcgcc
     9361 accgtatgga tcaggatgta gcggaggctc cccttctccg cctccagctc tgcacccgtg
     9421 cgcttctcga agtccttggg gaccgagatc gggcgcttca ggatccggct ggcctgggag
     9481 gcggcctccc cgggggtggc ccccttggcc aggaggagga ccaggcggga ccgctgggct
     9541 tccgttccct tttcctccgc cagggcctcc tcgatggcca ggcgcaggtt caacggatag
     9601 gacaggaggg tgtcctggag ggcgtccacc tggtcctcgg tcagggtcac ctcggggacg
     9661 gactcgtcct ccccttcccg ggggggctcc cggaaggccc gggactcccg gtccgggcgg
     9721 cccggaccgg agccgccctt ggccttctcg gcggcgggct tcttggcggc cggagcgggg
     9781 gtctcgtagc cggggatgga gaagtccgcg gccccccagg cgctctccag gttgaagttc
     9841 tccgacgggg tgagatcgtt ctccagggac gcgagttcct cgtccaggtt cagcccgccg
     9901 gccccggccg cggggggcgg cgcggaggag agctccgcct cgtccaggcg gaaggtctcg
     9961 aaggaatccg agggttcttc cggcgcgggt ccgggacccg gggagagttc cccgaagtcc
    10021 gcggccggtt cttccggcgc cgctccggcg gcccggaggg gttccgggga ttccccgccg
    10081 gcatctccca gttcggggat ggagacgtcg aagccctcga agtcctccgc acccgccgcg
    10141 gaggctccgg cctcggagag gtccgggagg ggctcctcgg gcaggccgaa atccggcagc
    10201 cccggggcgg ccggctccga ggactcgggc gcttcctcct ccccgaagag ggaaacctcc
    10261 tccggggcca gggggatctc ctcgaaaccc gccggttctt ccgcggcggg ttccggtgcc
    10321 gcggcctccg gctcggacag ttcgaaggcg gaaaggtcgt ccacgattcc cggggtctct
    10381 tccggagcct ccggggcagt ctcgaagtcg gtttccggga actccgggac cggggtttcc
    10441 aggtcttccg tgaggccgcc caggagcccc tcggggatcc ccccctcgtc ctccgggacg
    10501 gggacctccg gcgcttcccg gggccggtcc gcgagccctt ccgttccggt caggtcctcg
    10561 aggcccccga agtccggtat ggatttgccc agggcttcca cggatgccgc gccctccccg
    10621 ccttcctccc cgaaggtctc ccgcggagag aacgcctccg gggcctccag tccggccgcc
    10681 aggggggatt cggactcctc ccctcccggt tcggcgccca gggccaggtt gtccaggaag
    10741 gaagccaggt ctccctccgg ggaagccggg gccgcctccc ctccgggcgc ggggcccatg
    10801 agctcggaaa ggtcctgagg caggccctgt tccggcggcg ggacgtcctc gtaggcctcc
    10861 ccccactgct ccaggagttc cgactcccgt cccagggcgc gtatatcctg cttgaagcga
    10921 tcgatatcct cgaggttagg catgtcgtcc catcccctat attaacggaa ggttcatcgg
    10981 gctgtatagg gaaatcgcta aaggacggcg taattccccg ccgatacagt acttgaacgc
    11041 catgaagcac agaaccgcga tcgccgccct cctcctggca ttggccgccc aggccgccgg
    11101 agccttccag gtggagtccc tcagccttca cctccacctg gcgggcttga agaaggccgc
    11161 tcccccccag gtcgtggagg gctacttgat cctgaccgcg gaaggttcct tccgccacgt
    11221 cggagcctcc ttcgcccacg agagcttccg gaccgtgcac tccttccagc ggaacgccca
    11281 cggcctcttc atcctggccg tgcccgtgcc ctacgaggtc cgggaacccc tgaagtatcg
    11341 gctggtggtg gacgggatct ggggacggga cgccgtcaac ccggagttcg agcgggatcc
    11401 ccgcacgggg gccgtcttct ccctggcggc ggtgcccttc ctctcctccg cgcgccccgg
    11461 cgcctggaac ctcgtggcgg aggacggacg gacggctcgg ttccggtacg aatccgatcc
    11521 ggggctttcc gtcgccgtga taggggactt caacgcctgg gaccccttcg tccacgagct
    11581 gaaggagacc cgccccgggg tctacgagct cgccctgccc ctgccgtccg gggagcaccg
    11641 ctacgccttc ctggtgcggg gggaccgcgt cccggacccc ctcaatcccg cccggctgta
    11701 cgattcctcc ggcaggaccg tctcggtcat ccggatccgc taggagtttg tcgggctcct
    11761 aggccatccc ggccgtcgac gacggccgga ggtcccggcc tatcggtcgg cccgggacag
    11821 gagggtgtcc aggtacctgt agctggactg cagggcgccg aagaccgcgt gggtgtcctg
    11881 ggcggactgg gagatctttt ccagggattc caggccgtcg gtcagcgcca ggttctcctc
    11941 cttcagggcc gaggatatct ccgataggaa ttcccccgcc tgggtgacgg accgggagtt
    12001 ttcccggaag gcttccagga tccccgtaaa ggactcgtgg aaccgtccca gcagggccag
    12061 gaagccgtcc atcctctcgg aaaccgcgtc cacggccttc cggaagtccc ccacgttctg
    12121 gtcgatctgc ttggcgaaat ccccggtctg ctgggccagg gtacggacct cgttggcgat
    12181 gatccggaac ccgcgcccca ccgccccggc ccgggcggcc tcgatggagg cgttgatggc
    12241 caggatgccc gtgcggtccg agacgtcctg gatcgcgccc gtgatgcccg ccacggcgga
    12301 ggtcttgtcc ttgatgttct ggaagagtcc ggccagggtg cccgcgtccc gggacgcctg
    12361 gacgatctcg ccgacccggg cctcgatgtc cccgttcatc ccctcgttct tgcgcaggat
    12421 ctccgccatc atggaatcga tccgggcgga gttgccggcg gtgctgccgc tggtggcccg
    12481 gatctcctcg aaggccgaga cgatggaggc cagggccgcc ccgatccggc ccatggagtc
    12541 gtccaggatc tccagggaat tggcggtcac ggcggtcagc accacgctct tgtggtaccc
    12601 caccacgaag gcttctatcg cggcccggta gttcccggac ggatccgccc ggggcggctc
    12661 gggaggcatc ctaggctcca tagaacctgt ccaggaggtc cgtgccgcac ttgagggccg
    12721 agagccaggc gaggaagccc tggaccgagg ctccccggta gatagcgccg tgctgggggg
    12781 cgatcatctc gggctttagc cgggagacca tatcgcacca gcgccgggcg acggagttgg
    12841 aggcgatgag ccgttcgtgg aagccctgga tcaccggcag gtgggccgaa aagtcgtcca
    12901 cgaaggccgt ctcccctccc tccgggaaaa accctccccc gacgtccccg gagaagagga
    12961 tccgcgcctg gttgtcgaag aggctgaagg cccccggaga gtgcatgtaa tgggtcggca
    13021 cgaaggacag ttcggccccg gagccgagcc ggacgccctt gcccgcctcc tcgatgggct
    13081 ggatccgccc gggatccacg atgccgaagt ggggcaggaa ccgggtccag agtccggaga
    13141 tgtagatccg ggccggggtc acccccagcc acagggcgat cccgctggaa acgtccgggt
    13201 cctggtggga gaagaagatc ccgtcgatcc ggtccatggg cacgtggcgg ctgaccgccg
    13261 cgaccacccg ggcgaagagg tgaacccccc cgggatccag gagccagccc cggcccttgt
    13321 ccaggatgag gtactgcatg gtctggacgg cacccttccc ggaacgggca tcggcaccca
    13381 gccagatgaa ggaatggtcc ccgtcttcgt agagcacacg gctcgtcaga tccgcctcgt
    13441 cgttcatcct ttccgtcctc gttctcccaa atcttagctc cgaagtatat aatacccgcc
    13501 atggaagcgc aaagccgcgg catcggaaac cgcctgatcc gggccctcgg tgtcctgacc
    13561 gagtcggccc ccggccgggg agccctggcc gtgtccgcgg cggcctcctt cctttccgcc
    13621 cggagccccg gggccgccct ggtcttcgtg ctcctgcccg cctacgtcgt gctgacccgg
    13681 ggcgacttct caaccgccgc cggacccgag gccggagctg ccggaaggag tccggcgggg
    13741 gagattcctt ccccctcgga aatccccctc cggctggccg ccgccgccct ggccgccgcc
    13801 gccggcatcc ttgccctggg catcggcggc accatagccc agcgcttccg tacccaggtc
    13861 ttcacccttc cctgggggct cggagcggcc tgcgcggccg cctccctgat cctggccgcg
    13921 gtccacctcc ccttcctggc ggccttcggc aggcgggcgg gcaggatcgc ggcgggcacg
    13981 gtcttcgccg ccgccgggat cgccgccgcc ctgtcctcct ttcccgtcgg gtccctctac
    14041 gcggccttcc tctggcttct gtactccggc agcgccccgg ccctgtacct tcccctggtt
    14101 ctcggggccg ccgccctggc ggggctttcc gcggccgcgt cccgggcgat cctccgggca
    14161 agggcggccg ggcgatgaaa tcccccttcc gccgcgacgg gctcggccgc gccgtcgtcg
    14221 gcgccctggt cctgcccggc gcgggggccc tcctggccct ggcggcatgc cgttgggggc
    14281 cggcgttccc ggcctgggcc gccttggggg ccgcggccgc gggggccttc ctgtggtgga
    14341 acctcaaacc cctgcccgta cggacccgga acccggcgcc gtcctccccg gatacgggga
    14401 gcctgatgca ccccgacgcc gcccccttgg ctcagctcaa gggtcaccgg gacctcctct
    14461 tctgcgtcca cggcttcccg gccaccccgg cggaattccg gcccttcctg ccggaggcgg
    14521 acgtcctggg ctacgacctg gcggcccccc tccttcccgg ctgcggcacc cgcccccggg
    14581 acctggccgg ccaggactac gattccttcc tgcaccatgt ccggtccgag tatctccgtc
    14641 tgcgcccccg gtacgagcgg gtcttcctgg tgggtcagtc cctggggggc accctggccc
    14701 tggccctggc cgaggagtac tgcgccgttc ccgccctggc cccctccgcc ctggcggcga
    14761 tcggggcgcc ggtggtcctc aattccctgg tccggcacgg catggtacgc catcccctga
    14821 tctacgcggc ccgcttcctg ggactcttca cggcctgtct gggcgcccgg gttccggacc
    14881 cggaccggga gggggaggac ggggacgaac gctggctggg gtacgagggg atctaccccc
    14941 ggatcgtcca caccatgcag atccggttcc cggaggtgga acgaaagctc cgccgggtta
    15001 cctgccccgt cctggtctgc cacgcccggg gggaccggat gacggactac cggaacgcgg
    15061 cgatcatcgc cgggggggtc ggctctgacc gcatcgagac ctatacggcc aacatggacc
    15121 ggttccggca catccggcac tccctcctcc tgtacgattc ccagagggac cgggtccggg
    15181 aacggatcct ccggttcttc ggggaattcc ggagctagga gcctgtcgaa aggggaaacg
    15241 catgatcgag gtccgggacg gacgattccg ggacgccgag gggcggaccc tcatcctacg
    15301 gggagcgaac ctggggggca gctcgaagta ccccgcccgg cccgacggac gcacccacgt
    15361 ccgggagggc ttctacgagg ggcgggacgt ctccttcgtc gggcggccct tcccccgtgc
    15421 ggaggccggg gaacacctgg accgcctcaa gctctggggc ctggacttcc tccgcctcct
    15481 ggtcccttgg gaggccgtgg agcacgcggg gcccggcctg tacgacgagg aatacctgga
    15541 ctacctggag ggcgtggccg ccgccgcgga agaacgaggg atccgcctct tcgtggaccc
    15601 ccaccaggac tgctggagcc gctggaccgg gggggacggg gccccttcct ggaccctgga
    15661 ggaggcaggc ttcgaactgc ggaacctcca cgcctcgggc gccgccttcc tccaccagga
    15721 gcacggggat ccctgccccc ggatggtctg ggcggccaac tacgcccgcc tgggctgcgc
    15781 cacgatgttc accctcttct tcggggggaa ccacgcggcc ccggagcttc gggtcgaggg
    15841 cgaatccttc caggagcggc tccaggggca ctacatccgt gccatggcta ccgtggcccg
    15901 gcgtctggga cgcttcccga acgtcgtcgg cttcgactcg atgaacgaac cctccgccgg
    15961 attcctgggg ctttcggacc tgcgccgcct ggagcgggcc ctgacccgca cgggccccat
    16021 gcccagcccc tgggaggcca tccgggcagg ggacggccgc gccgtggagg tggaccgcta
    16081 cggcatcacc ccccggggcc agtccgtggt cggacgcgcg atcctgggac gggagggggt
    16141 ccgggcctgg cgggagggag tcgagtgcct ctggaagcgc gcgggggtct gggacgaggc
    16201 ccgcggaaag cccgtcctcc tgcggcccga cgccttttcc cggacccggg acgggcgtcc
    16261 cctggatttc ggggaggact gcctcaagcc cttcgcccgg cggtacatcc gggaggtccg
    16321 ggccgccgcg gaggggggcc ggcgcttcct cctcttcctg gagaccgttc ccatggacga
    16381 gcctccggcc tggagccccg cggacgatcc ggccgcggtc aacgcggagc actggtacga
    16441 cgccctgacg ctgacgctga agaggtggtt cggatttttg gggtacgacg gggaggacgg
    16501 ccggatcgtc ctgggtagga aggccctccg gagctatttc cgggaggccc tggagcgggt
    16561 ggtccggcgg gggcgggagc ggatgggggg gatcccgagc ctgctcgggg agttcggcct
    16621 gcccttcgac ctggacgggg gccgggccta ccggacgggg gatttccgga aacacgagga
    16681 ggcccttgcg gcctaccacg acgccctgga cgcggcgggg ctgtcgtcca cgatctggaa
    16741 ctatacggcg gacaacacga acgagcgggg ggacggatgg aacggggagg acctgtcgat
    16801 ctattcatcc gaccaggccc gcctgcccct gcccgccggg gcggatcccc gggaggcggg
    16861 gggccgggcg atccggggct tctgccgccc ctacccccgg gcaaccgccg gcgacccgca
    16921 gtccttccgc ttcgacgccc ggaccgggga cctggagtac cggttcctgc cggaccccgc
    16981 cgtcgccgcg cccacggagc tctacctgcc ccccgtgcag tacccggagg gctgccgggt
    17041 cgaggcggag ggcgggacct tcgaaccgga cctcccgggc cgggtcctca aggtccggcc
    17101 ccacccggga tccgcggaag tgaccgtgcg ggtccggcgg gcggaccgga agccccggta
    17161 agggtcctcc ggaccgccgc tccgggtcta ctggctcttc cgccgggagg gctggggcag
    17221 gcgccccttg ggggtcttct tgaccagggc gtcgtcgaag accaccacgg tcccggccat
    17281 gttgatgacg tccccgggct ccaggcgctt gcgctcgacc tcccggttgt tgaccgtgag
    17341 gttcccgtcc cccaccacgg tgtaggcgtt ctgcctctcg tcgaagagga tcgtggcgtg
    17401 ccgctccttc agggaggggt tccccccgat cgtgaagtcc gcggcgtcgg aggatccgat
    17461 cacggtctgc ggggagttca gggtgaagac ccgggttccc ccgccggagg gcccgtagag
    17521 gagccgaagc cccgcctcgg tggtctccct ctccagcttg aacaccagga gggccagcca
    17581 caggagggcc ggcacgagca ggaaggccag gtaataccag ccgggaaggt cccggctcca
    17641 gcccaggacg gtgccggcgt agtagtatcg ctccgaggag aactcccgcc cctcccggtc
    17701 ccgcaggacc gcccggacgt tccggcggct gccgggggcc atgcccgccc ggtagtccac
    17761 ggcgatctcc gccaggacgt ccgcacggat cgtggagtag accgcggcca gctcgtcccg
    17821 gttcgaggcg tcgaatacct tgcccccggt ccgcaccgcc gcgtccccca gggaggggtc
    17881 cttctcgttc ccgaagcgga ccacgtacac ggtgatcccc tcccgggcgg ctcgctccac
    17941 cacgtcctcg gggcccgtgg tccgggtccc ggtctcgggg ttcggcttgc cggttcggat
    18001 gtaatagggg aagttctccc cgtccgagag gaccaccagg gcacgccgtc cgggcacccc
    18061 cgcgaagtcc gccagggcgc ggtccaggga gaggtagagc tccgtgtagg cgtcctcggt
    18121 cgcgggcttg cggatctcgt ccaggccccg ggagatcctc tcgaaatccc ccacgggatc
    18181 cgcggccttc cagtagcggg tgttgaacac ccccaggccg atccggtcct tcccgcggcc
    18241 ctgccgggac gcctcggcca ggaaggcccg ggcggcggcc ttggcgtggg tcatccgctg
    18301 atcctcggtc ggtgcgcctt cccggccgtc caggccggtc cacatgctgc cggagttgtc
    18361 caggaggaag cagaaggcga tcccctccgc gtcgttgatc ccccgggata cggcggtcac
    18421 gggcctcgga gcccattccc cgccctcccc ggattcccag acctcgacgt ccggggactc
    18481 ggtcccggcc gccccggggg cggagaggta gagccgcacg gactggccga acaggagccg
    18541 ctccgtgtcg atttgggtga tggccagccc ctggcccgcc cccggaaccg ccgccaaggc
    18601 ggcgatcagt attaggaaac cgtatcggaa agctctcatg atcctcaccc ccgccttcgc
    18661 gtcacgcgaa gtagccgtac aggaatttcg ccttgcccgc ggccaggacg tcctcgggcc
    18721 tcagggccgc gtcccccgcg ggtccgtcgt tgaccaggac cccggcgtcc ggtcccgcgg
    18781 gtcggatata gacccggccc ttctccaggt ggaccgtcgc gtggaggggc cgcaccccgg
    18841 gatatccggc gaggacgatg tcgcaggagg cgtccgtgcc caccgtgagc ttccggctca
    18901 ccaccggata ctccttcccc ttgagcggcc cgttcaggag cttcagggtc cccgcctgga
    18961 agcggttctc gaagagagaa tagaagaggg tcaggaggaa cccgaaggct gccaggccag
    19021 ccaggaggga ccacttccct ccgggggcca gtacctggag ggccgagagg atcgcgcctc
    19081 cgatgaggcc gccggccagt ccccccagga gcccgaccag gagcttccgg cccgagcgtg
    19141 cccgaaggcc ctccgtcatg gccaggaagg tcccggcaat cgcccaggcc atcccgttgg
    19201 ccgccgcgag tcccgggccc aggcgggagc tgttcgaacg gaagagggcg cccccggcca
    19261 ggaagaggaa gagctggccc gccaggaccc ccgccgcccc ggccgccgcg ccgaagaggg
    19321 cgccgaagcc caggccccgg aaggccttgg accgggagga cacgacgatc ccctcgaagg
    19381 acccgaagag ggccccgaac acgaggccca ggcagacccc ctgggacaag gtgaagacca
    19441 ggtagctcgg gaacgcggcc tgggccctct ggaccatcaa tagaatgggc cagagcatca
    19501 gggcccccag gatccccagg acggccatga ccgcgcgctt caaggtaagg gacatggttc
    19561 actcctccac gcggaacctc agggtcccgc cttcgtcgga tacgcggatc cggaccgtgc
    19621 ggcccggctc cagccggaag gacgccctcc ccgaggcgcc tccccgggcg actcccagct
    19681 catacgcgct cgcgggcagt tcccactcga cccggccttc cttgaccgtc cccagccgga
    19741 gggggagcat ctccggaccc ccggaggccg ccgtgcccag gccgttcagg gtcagccggt
    19801 aggaccccgc gggagtctcc acgaccaggg acccgggccg gggcgccagg cgggcttcca
    19861 ggcgcaggcg ctcctgggcg ttggcgatcc gcagggagaa gacctcgggg aagtacccct
    19921 cggcctcggc ccgcagcttc cagaccgccc cgctggtcag gtccccggat tcgagcttgt
    19981 cgaggggctt ccactcgccc cggaggtagg ccgagaagac ggcctcgccc aggaccgtcc
    20041 cggtcagggc atcccgcgcc tcggtcagga ccgccagggg ccgggggggc cgatcctcca
    20101 gtcggaactg gaggacgcgg acgccgtcct cacccggggc cgccatggag tacggcagga
    20161 ggtggaagga ttcccaatga agatcctggt ccgcggtcag cttgacccgg tagggccccg
    20221 gggccaggaa gaggcggttc gagacgaagg tgtggaaggg atccgccccc tccgtctccg
    20281 ccggccgcag gacgatccgg acgtcctcga cctccggaat ctccttgccg tcgttgtgga
    20341 agatccgggc acggaggaag atgtccgcgg cctccgcctg ggacttcgga acccggacca
    20401 ccaggcgcag ctccccccag cggtccggca tcaggtaccg gtggaccaga ccctccgtcc
    20461 atgcgtacca accccccgcc ccgatccccg ccaggaggac caggatggcg gccaggagga
    20521 aacgcttcct cggggcgggg cggtagcgcg gctcgtcctg gcacttcccc gacacgaggt
    20581 ccgagagaac ggagcgcagg gagtccgcgg agtagcggga caggaatttc tcgaccgtct
    20641 tgatgaccgc gtccatggtc tggaagcgct tgcggggatc cggcttgatg agcttcttga
    20701 tcagcttgtc cacgaagggc aggaccctgg gattgacctt gcccgccgcc ctgtaccggc
    20761 ccttttggat caggaccacg gtttccggcg cgaagttccc ggggtagggc ttcttcccgg
    20821 tcaccatctc gtagagcatg atccccatgg cgtagatgtc cgcccgacgg tccacgttct
    20881 tggagttctc gatctgctcc gggggcatgt aggaaggggt ccccagggtc atcccctcct
    20941 tggtcaggcc cgagtcgtcc tcctcctcgg aggccgcgat cccgaagtcc gccagcttca
    21001 cctcgccctt gcgggagatg aggatgttcc cgggcttgat gtcccgatgg atcaccccca
    21061 tcccgtgggc gtactgcagg gcccggcagg cgtccaggaa gatgaccaag gccagttcgc
    21121 tggacaggct ccgctggcgc ttgatcaact ggtccaggga cattccgtcc acgtactcca
    21181 ggacgatgta gtgggagctt ccttccttga aatggtcgaa gacccggacg atgtggtcgt
    21241 tcttgaagtc catgaggatc cgggcttccc gcttgaagcg ctccgtgatc gtggcgctgc
    21301 cccgcagggt cagcttcttg atgatcacgt ggcgcttgag ggtcgggtgc agggccttga
    21361 acacggcccc catcccgccc ttggcgacca gggattcgat cgggtacttg ccgatcagct
    21421 cggggaacac ggccatatcg atctcctatc tctcggactt ccgcgggtcc gctgtgcggg
    21481 gccttttcag gtccgcggcg tggacctttc gcaggtccgc ggtgatgtct cgaatctcac
    21541 cgatatccag ttccgggtcg aagtcccggc ccggcatgac ccaggctacc acgaacctgt
    21601 ccgggttgtg gatctgcaga tgccggccgc cggagagctc ccggtagacg ccccggatgg
    21661 cccggtggcc cgtgaagtag cgggctcccg ggatgcccgg aaggaaccgg tccaggagga
    21721 ccggaacagc ccggggatcc gcggctccgt tgtccgtcca ggtcaggccc aggaccgtcc
    21781 cgggatccag ccgggcgtcg atcagctcca tttccgaata cgctcgttgg ggctccgcgt
    21841 gggacgcgag gaagcggggg cccgtggcga acaggggcag ccgccgctcg aatcccgcgt
    21901 agcgctccag gaagtcccgg ccgtagaatc gctccagcca ggaggtcacc atcatcccct
    21961 ccagggcgaa cttccggaac ggatggttcc cgttcccctc ctcgttcagg acgttctcgt
    22021 ggttgccctt gaggaagtgg aagcgccccg gaaaggccgc cttgcagacc aggaccatct
    22081 ccatgaggga aaggctctcc cgcatctcct ggtccatggc cttgtgcttc gcgtagcctc
    22141 cggcgaattc ctggtaggcc cgcttccagc ggtccaaggc ccgggactcc gcgtggaagc
    22201 cgtcccccag gcagaggatc tgcagggccc cctcccccag ggcgtccagg accgtctgcc
    22261 cctccggcag gggctggaaa aggacactgg ccaggaagtc cgtccgggcg tggaggtccg
    22321 ggagcaggat ggtcggggcg tccccggcca ggcgcaccag gccccccggc aggccccggg
    22381 aatcccgggg cctgccgggg cctccctcgg cgtccagggc gtccgaggcg gcctccacct
    22441 tggccaggta ctcctcccgg tcgggcaggc cccggcgaag gaaggccgcc cggaccgctt
    22501 ccgcggcgga catgctcgtt ccgccccgcc tactcgatga cggtcttgtt cacttccacg
    22561 aagtcctcgg ccagcccctg ggacacctgg gtcaggaggg cttcctcctc ctccgcctcc
    22621 agcttggcct tggccaccgc gatcttgaag accggggagc tcgacttgat cgggcccttc
    22681 tcgaagcggg cgggctcgtc ggcctccagg tccgcctcca ggaccgaggc ctcctccacc
    22741 gcggaggcgg ggacgtcggt tcgcatgccg atggaggtgt actcgcagcg ccgcaggccc
    22801 tcgacctggg ggccgatgtt gaggagggat cccgagtagg tcaggaccag ggccccccgg
    22861 gcctcggcgc ggccgaagaa ggagacctcc tccaggtcgt tgcccgccag ggcctcctcg
    22921 aaacaggtct tcttctccag ccaggcgtcc gccatcatct cgtaggtgtc cgggcccgcc
    22981 gggagtcccg aggtcttcgc gatctgccgg atatggtcct ggatgatggg ctccaaggtt
    23041 tcgaacgtgc tcatgtgttc ctcctgtcgc ccctaagata cagcattctt ttgaattttt
    23101 cacggtattt tacgagaaaa aatgaaaaaa cttggatttc gtggtatagt aagggggtag
    23161 ggtgcggccg cgggacctcg ggccggccaa aaactcagga gtttttcagt cagcccggtc
    23221 ccgggagagg gggagggaat gaaggaagtc aaattcgagg accttccgga ggcgatccgg
    23281 aagcagttgc tctcgctgat ccaggactcc ggcctggacg gggacgagga gacccggaaa
    23341 cgcctcgcgg tgatctggga cgagaagtac cggctcttca cgggtcaggt gggggccctg
    23401 ggcatggagc tcgtcccgga gctcgggaag gaggataccc ggggggccgt cctcctcacc
    23461 tactccgggt ccctcatcag cctggggccc ctccggggca ggagccgctg gctcgagtac
    23521 gcctccatca agctccggac ggacgtgccc gagctcgtgc ggggagacaa ggcgagcctg
    23581 gcgggcccga tccgcctgga ccaggcggcc gtcttcgagg gctgccccgt caagcggtcc
    23641 tcggctctct tccgtatcgc cgtctgcccc gagggtctct ccgcggacga ccaggaacgg
    23701 aggatccggg aggccaccgt ctacctcacg aacggcttcg tgaagctcaa ccgcacccac
    23761 tccctcgagc ccgtcccgga ggtggaccag ttcaccgcca aggccatcat cggctacatc
    23821 gccaagaaga acggtgtcac ccagctggcc gcccggggcc tcatcgagga ctacctgtcc
    23881 atggtggaga ccgggatgct cctgggcgag cgggtctcgg tcggcaagct cggcagcgcc
    23941 tccttgcgct accaggcggc ccggaaggcc cggctcacca ggaacctgcg gaccggggag
    24001 gagctcctca tccccgccaa acccgcctgc ctcgcccccc gcttcacatt ctcccaggcc
    24061 gtgaaggaca agtgcggctc cgtggatccg accctgttcg gggccgagga gggcggggag
    24121 gacgaggagg actgagggcg tccgaaggac aggctccaga ggcgggatcc gcagggtacg
    24181 gagcccgccc gcgatatact ttctccgaac ccgaggaagg agatcccatg gacccgaccc
    24241 gacgcgatcc ggaaaagagc tacctgttcg ccgccctggg ggccttcgac gccgcggtgc
    24301 tgtccgccct ggcctgggcg gcgatcacgg tgctgaccaa gtaccagatc ggctacatgg
    24361 ccgtggcggt gggcgccctc gtggggatct cggtccgctg gttcgggcag ggatccggca
    24421 aggccttccg gatcctgtcc gtaggcttcg ccttgttcgg ctgtgtcttc gggaactacc
    24481 tctcccaggt cgggttctac ctggccgaat ccggggcggg cctgctggac accctgctgt
    24541 ccctgcgccc ggcccaggtc cccgcgatcc tggccgagtc cttcagcccc atagaccttt
    24601 tattctacgc cctggccgtg ttccaggcct ggaagttatc cgtggcgccg gccgctccgg
    24661 aggcggagac ggccgccgcg gcggagggat ccgcgtcgga cgcggaaccc cggcccctcc
    24721 tgccccccta cccgccccgc aaggccctgg ccgcggccct ccccttcctg gccatcctgg
    24781 ccatccccgt ctacgtggcc gcgcgaagcg acgggacggc ccgggacgtc tacgactcgg
    24841 gctccccgaa gtacgagggg ccgaccagct ggaacgagcc ccacggcgtc tggaaatact
    24901 gggacgagag cgggaccctg ctggcggaga tcgaattcca ccggggcgtg ccggagggcc
    24961 ggtccgtcca ctacgccgag gacggctcga tgtccgaacg gaaaacctgg aaggccggct
    25021 tcctccacgg accctacgag cagttctatc ccgacggcac gcttcgtgcg aagggcgcct
    25081 acgaatacga ccggaagacc ggaccctggg aggaatacca cccgaacggc cggcttagct
    25141 cttcggggac gatgctgctg gatctccagc acggcacgtg ggagatacgc tccgactccg
    25201 gcgccctccg ggcgcggacc gccttcgacc agggggagcc ctcgggcgtc tgggagggat
    25261 actcggagga ggggatcctg acgctccgga gggatttcgc ggggggccgg gagaggatcc
    25321 tgtaccagcg ggacggggac cgggagactg tggtcgacgg ggagggccgc tacgtggagc
    25381 ggtacgagga cgggacccgg gcggtcgagg gaccggtcgc cggggggatc aagacgggaa
    25441 cctggcggga ctggtacccg ggcgggaaac ccaggagggt tttcgagtac tcggacgggc
    25501 gggaatacct caaggactac tacctggaga ccggggagcg ggccgtgcgg gacggcgacg
    25561 gaatcctgtc gaccggggag gagggcggcc ggatcgaggc gcgctacgag aacgggctcc
    25621 tgaccgggga actgaagacc tactaccccg acggaagccc cctcggcctg tccacctact
    25681 ccgcgggcct tctggacggc ccccagagaa cctgggcccc cggcggaatc ctcttggtgg
    25741 agggtgagtg ccggggcggg ctgcggacgg gcgtctggac ctggtacgag gaggacggct
    25801 ctgtctcctc caccgtgacc ttcgcggaag ggaaaaaaca gggcgtccag accttcttcg
    25861 aggacggcgt cctcgtcaag gaggaagtct accgagacgg ggcctacgtg gagacgcggc
    25921 ttcggcgctg agggcgacag ggtccggagc cgggcgccgc cgcggcgggc ggcccggaag
    25981 gccgctccgg ggcccgggac ctagaaggat tcccccgggg tgaaccggag cccctcccgc
    26041 acgtagtgca cccggagctt ccacgcgtcc ggcacggcct cggcgcggag gacctccccc
    26101 atcacgaccc cggaggtccc ctccaccacg atccgggaca ggcggcactc cagccagccc
    26161 gcgacgccct cggggatccg ggcgtccacc tcccgggcgg gaaaggcggg gatcccgagc
    26221 tcggcgtact tgtccgcgtc gtgtcccgac atcgagcctg cgcgggcgac caggtccatc
    26281 tggcccttgg tcggcagggc caccgcgaac tccccggaat ccgaaaggtc cgcgtaggtg
    26341 cggtgtcccg tgtccaggac caggatgagc ttggaggcgg gcgagtagtc caggggacag
    26401 caccaggcta cgggcgcgac gtcatagcgt ccgtccggac cccgggtgca gaccaggacc
    26461 aggccgcccg cgttctggag ctcgtaggcg tcggagacgg ggatctctgc gtaccgcatg
    26521 gtcacctcct cgatcgcggc cttcgccgcg cctcatcgcg tcgcggcccg ccgttccgaa
    26581 cggtccacca gcaggaaacc gccggccgcc gccaaggcca ccgccccgag caggatccag
    26641 acgggcccca gtccggaacc ctcgatgatg cgccccgtca ccggcgaact cagggcgaag
    26701 cccatccccc cgatgagggg caggatcgcg ttgaaccgcc cccggtggga catgggggta
    26761 tggttggcga cgtagacctg ctcgttcgtc gcgttgacga tctccccgaa ggtccagatc
    26821 gtggtggaca ccaggaacag cgcggggctc cggatgaagg cgatcatccc gaacccggcc
    26881 atgtacagca aacccgcgac ggcgacgttg accaccggac ggaaccgttt caaggcggcc
    26941 accagggggg cgttgaagag gatgaccagg acggcgttca gggtcatcaa ggttccgtag
    27001 agcggcgcgc ctttctctgc gaaaagggcc tcgagttgca ggggcagggc gaaccggtgc
    27061 tgggcgtaga cgaagccgta tcccgcggac agggcggtga atatcaccag gaagtaccgc
    27121 gaccggagcg cggagaggag attcccctcg tgggccctct ccgtcgagtt cccggccagg
    27181 ctctcccgga tctgatggtc cgacggcttc gattccggga tgaacaggat cacgagggcc
    27241 agggacgcca gcaccgcgat cccgttgccc cagaacagcc agctcatcgc gttctcgaac
    27301 aggaagcccg cgatcagggg gccgaacgcg aatcccaggt tatgaccgag gtacaggagg
    27361 gaaaaggcgg cctggcgatt ctccggcgtc gtcaagtccg tgctcatcgc ggaacgcgcc
    27421 gggtccgtga cgccgtcgaa gaagatggaa accaggatga gccagggaac cagggcgtgg
    27481 gctcccagga atccgcacgg gatgaagagg agtccgcaga gggcctggga tacgagcatc
    27541 acgcgcttcc gcccgaagcg gtccgccagc ctgcctcccg ccaaggttcc gggaatgtag
    27601 gccaacgaga caaggagcag gaaatccccc gcggctcgct cgcccatgcc cattttcttg
    27661 gtcaggaaca aggtcaggaa cgggaagaca aagactccga tcccgttcac cacggtcgcg
    27721 aagaacaggg tgtagatgga ggccgggagg ccccggtaga tggagaagga atggatcgcc
    27781 gatcgaagca tgcggcagta tcgcatcccg gggtccgtcg tttccagccc cggcgcgcac
    27841 cggaatcccg gcacctatcc gggcgatcct ggcattcccg ctcagttccg tcgcgcgatc
    27901 atctcgatct cgaccagggc gcccagcgga agctcgcgga cacccacggt gctgcgtgcg
    27961 gggtacggcg cctcgaactt ggacttgtag acctcgttca tcgcctggaa gttcttcatg
    28021 tccgtcagga acacgttcac cttgacgaca tcgtccaggg tgagacccgc cgccgcgaga
    28081 acgttcttca ggttctcgaa gcactgcgcg gtctgggcct cgatcccccc ctccgccaag
    28141 cggctcgtcc ccgggatcgc gggagtctgt cccgagaggt agacgaatcc ccccgcttcg
    28201 acggcatggg agtacggacc gacggctacg gaaccgggag ccgtgaacgc ctttctgggc
    28261 atgactggga acctccttga ggggcgccgt tcatccggca cacgcggacc atagtacgcg
    28321 gcctattccg aatccacaag cctctgaagc agggcgacgg agacctcgcg gatgtcgtcg
    28381 acgatcctgt ccgcgagcga caaggcctgc ctccccgagt tcgggttccg gaatccgatc
    28441 gaccggatcc ccgcctcccg ggccgcggtc aagccgttgt cggaatcctc gatgacgacg
    28501 caggatcccg gctcgacccc gagcagctcg gccgctcgga ggaacacgtc gggtcgaggc
    28561 ttcccgcggg ggacctcctc gccgctcacg acgacatcga acaaagaatc gagttcgtac
    28621 ttcgacagga ccgtctcgat atagtcccgt gtcgaggagg aagccaccgc cgtccgaatg
    28681 cccctgtccc gcaggtcgcg gagcaacggt agcacacccg ggataaggat ggactccgaa
    28741 ccttccagca tctcgacgtt cgccctgtgc tgaagttcct tgagcgcttc gaccgactcc
    28801 gcgaatccga accgctcgcg catctccctc cacatcacgg agctcgatat gccgatgaac
    28861 ctgtccaagt cggaggcctt gagatccacg gatcgatccc ggagcagtcg aagcacggct
    28921 tccgtatgga gaggttcgct atcgatgata gtaccatcca ggtcgaacag aaccgccttg
    28981 agcatcccga tcccccaatc tccggtcacg actctttcgt cgaaggtatc ggaaagaccc
    29041 gtgtcggaca agagcatcgc ggtgccccga cagatcgtca gactcttcgg gctccgaggc
    29101 tctcctggac aaagtccggg gtccccgcaa tccccacccc agccgtctct cagaggaact
    29161 ggaacaagtc ctcctcgatt actgcctgga gtacccgacc caagaaagcc tcagggtcag
    29221 ccaacagctc cttctccggg ggattcacat cggagcggga gccatccgcg gcgtctggca
    29281 acggcacaat ctgttatcca
//
LOCUS       DAOTEI010000023        28800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1073_length_28800_cov_5.902105, whole genome shotgun sequence.
ACCESSION   DAOTEI010000023 DAOTEI010000000
VERSION     DAOTEI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 28800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28800
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_28800_cov_5.902105"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="P5313_03740"
     CDS             complement(<1..716)
                     /locus_tag="P5313_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRY79510.1"
                     /translation="MSKDYFHRVHRLTPTKFWINNPTRAEAATAIAAGALGCTNNPSY
                     TWKMLAHPEEGPYAGKILDESIRVSRSDDEAAAIFQRRLVAPVAEAFLPIWKASHGAD
                     GWVSIQGDPVHEDDPEVIIDEARENRKVSPNICIKIPTTEAGLEAMEVLIAEDTPINA
                     TEIMSVAQGVALCETYNRVSRKTGRKPPLYLSYIAGIYDDHLQNQVKAQGIPIDRDVL
                     NQAGLAGARKMYDIMTERNYP"
     gene            complement(776..1465)
                     /locus_tag="P5313_03745"
     CDS             complement(776..1465)
                     /locus_tag="P5313_03745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRY79511.1"
                     /translation="MLSPRSSEPLSGLRKVIADRLSRSKREAPHAYFSMRVDMGAAAD
                     LRRDLAARDRKVSYNDLALSAAARALVEVPGLNGRLEGETLSLYDTVNLGMAVSLEKG
                     LIVPVLAGAERLTLVELADKARDLAGRARLGKLKLSEYSDGTFTVSNLGMFGVGQFAA
                     VINPPELGILAVGAVTEEAVARDGVLTVRPVLTATLSVDHRAVDGALGGRWLAAFRDI
                     LQAPRERLAVE"
     gene            complement(1470..1715)
                     /locus_tag="P5313_03750"
     CDS             complement(1470..1715)
                     /locus_tag="P5313_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79512.1"
                     /translation="MALKMISMPKVSQEMEEVVLYTWFVKPGDSVKAGDPLFSAETEK
                     TTVEVESEYSGKITRLLVAQGDTVKLFSEIAEIDTED"
     gene            complement(1801..2565)
                     /locus_tag="P5313_03755"
     CDS             complement(1801..2565)
                     /locus_tag="P5313_03755"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY79513.1"
                     /translation="MDIDLKGRPVFVTGAAKGIGRAIAKVLAECGAPVAVADLDEAGA
                     RETAAAVTAAGGKARAYKLDVTDERAVGAAMDAAEKELGPLYALVNSAAILIPPRPFH
                     TIDLKDWEAIFSVDVKGLFLCCKAIAPRLIERRRGVIVNIGSVAGKIQRLNMAAYCAA
                     KASVIHFSKVAAFELAPHGVRVIAQCPGTTATDMVLKTITGGDPEALAKWLAGIPMGR
                     LAEPEDQARLTAFLISDLASHITGQAINVDGGQVMW"
     gene            complement(2586..3575)
                     /locus_tag="P5313_03760"
     CDS             complement(2586..3575)
                     /locus_tag="P5313_03760"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRY79514.1"
                     /translation="MAELTYGKAIGRAIAEEMRRDPDVFVMGENVGTTGNIFKVLEGL
                     LAEFGEERVRDTPLAEEIIINAAVGSAMAGLRPVAEIMYVDFTTFGLDALLNQASKSW
                     FNSAGRVNCPMVLRCPQGAGPGRGSHHTNSLEAWFAHVPGLKVVLPATPADAKGLMVS
                     AIRDDNPVVFLEHKQLYFTKGEVPDGEYSIPLGKAEIKRPGKDLTIVCYSITVHTALK
                     AAELLQKEGIEAEVLDLRCLMPLDREAVLASVAKTKRAMVLYEAPVHYGAGGEIVTLI
                     QQELFRDLRAPVRRIGGLHTHIAYAPNLCAAAVPTAEQVVVAARDLVGYERKA"
     gene            complement(3577..4542)
                     /locus_tag="P5313_03765"
     CDS             complement(3577..4542)
                     /locus_tag="P5313_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRY79515.1"
                     /translation="MEDGKLLRQYEVMLRERYFVLEQCTQNSKGKIHGYLHCYTGEEA
                     IAAGICADLNPKDSVFSTHRAEGHLVSMGARFDRMMAESFGRVDGYGKGLGGVMHMSV
                     PEIGLVHSNGVVGGGIGLAVGAGLAHRYRKDGGVAVAFFGDGASSHGVLYEGMNLASI
                     WKLPVIFVLENNGFAISSPATKMISIADVARRADGFSLPGIVVDGNDLVAVYEAASTA
                     IRRARAGEGPTFLECKTYRTRGHNEGDPQWYYRTREDVKDWEQNRDPVKRCKALLISK
                     GLLTEEKDKAMVDAIKKELAEAVAFADASPYPGEEYLEKYVWKEQ"
     gene            complement(4561..5523)
                     /locus_tag="P5313_03770"
     CDS             complement(4561..5523)
                     /locus_tag="P5313_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79516.1"
                     /translation="MNKIAARFRAFAEVQRWIWAAAGSFLLWLGMGLVSSRLNLESLV
                     SNSMSAAFLAIAAIGQMYVISCGRGAIDLSIPGVITITAFLSTGIINAQDANILQGVA
                     VSLAAGALVGLANGLIILFLEIPPIIATLATGYVLTSASLIYNEGFRTFNSAPLLVTL
                     TRGRFLGVPFMFLVTGLLALAAYWLMKRTVYGRSLTAVGQNVEAAALSGIRVRRVQLA
                     AYVISGLFSGAMGVLLAARVSGAFLGMGDSYLMETIGAVVIGGTLIFGGRSTVLGTLF
                     GCLFFVIIVTAMQVAGFDAGVQKIVKGVLIILVLVVATEKPVRN"
     gene            complement(5520..7994)
                     /locus_tag="P5313_03775"
     CDS             complement(5520..7994)
                     /locus_tag="P5313_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY79517.1"
                     /translation="MESMTAREDPAVRVRLLSAEGLSKTYVSTKAVQDVSLSISAGEV
                     IGLVGANGAGKSTLMRVLSGVTRPDSGTLEVGGSTIRWDEYSPARAAGLGIRVVYQEL
                     SLSTNLTVYENFYVEHAARYRGPFRKWREEARKAARASLDAVFPGNGIDERQSLSALT
                     IAQKQMVEIARSVSDPGLRILILDEPTSSLGVEQTEQLKNYIWNRRAQGVSFIFISHR
                     LNEVMTLVDRVVVMQNGQVKWSGAVADTDEEDLVRRMGEAVLRERSVPGSRACVEPGA
                     GSAGECSARLTIRGLRTHALRDIDLDLRGGSIVGIGGLEGSGQRELLRAVFAPSGSAA
                     RSVERTGRVAYVTGDRKKEGLFPLWSIAENAVISRLARLGGIRPVGRTTGQESVRSWY
                     DRLKVVAAGPGAPITSLSGGNQQKVLIARAMISDADIVILDDPTRGVDIQTKLQLYEI
                     FREAADKGKLILWYSTEDEEFEICDEVHILRYGTLVKKLSRGEIDKNSVVNAYFSGEH
                     LKPEAASIAKVSKFDVSGIGIPLGAMLLVYIVSGILTPAVFSEFGITLLIGGSIPLIL
                     ATLAQMWVIGLSMIDLSVGAFMGLVNVIGATLLETNPAAAVACYAVMLLAYAGLGALI
                     HLRQIPAIIVTLGASFIWTGIAYTILSMPGGKAPDWLIAFYGFQFPVIPFVLIIVGVV
                     TAAAVLLHRSRYGTVLRGFGNNAAALERSGWSVLKAYMAGYFLSGVFGLLGGIFVTAV
                     TTGADANATLSYTLLTVAAVVMGGGDLTGGIVSPLGAVFGAITLSLIGALLGFLNLST
                     NLVAAVQGAILIAVLALRLVGRRRSV"
     gene            complement(8082..9095)
                     /locus_tag="P5313_03780"
     CDS             complement(8082..9095)
                     /locus_tag="P5313_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79518.1"
                     /translation="MRKTVLIALAILMAFGSMSAAAQQPKKGKWVIALSNSYYGNMWR
                     KQSVEAFEEAAKAAKAQGWIKDYVIVNGDGTQNTQISQMNSLILKKVDAICINAASPT
                     ALNGVIDQAAKAGVKVLAFDSIVSTPSAWTMDFNFVEQGEGGAQFVADKLGGKGNVLI
                     VRGVTGSAPDQDMYKGQMNVLAKYPNLKVVGTVYGEATTTVTQAAVANILPSLPQVDA
                     VLTQGGGDAYGAVQAFEAANRPMPIIIGDNSAEFIQWWMKKMNDGYSTYSICSAPGIG
                     GAAFWTALAILNKVDVPKKMSLKVIPITNANAKDFSTLKPGTLASPTFTWDYVMEKII
                     KAK"
     gene            9301..11121
                     /locus_tag="P5313_03785"
     CDS             9301..11121
                     /locus_tag="P5313_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79519.1"
                     /translation="MQKPNPSKPAAPGLSSDRLRLAAGPVSAVFEDGFLRYIRLGSTE
                     IVRGLYSAVRDHNWDTIPGRIRDFRMERGEDSFTLTYVSEHRRGSIGFTWNAEITGTP
                     DGTLSYSMDGTVLTAFRKNRIGFCLLHPMSLAGLPCRVTHSDGSREDGAFPSLISPHQ
                     PFLDMRRIEYEVGGIRVSQDFEGEIFEMEDQRNWTDASYKTYCTPLGLPFPVEVREGL
                     RIRQRIVIRAARTEGSPVRVEEPGTASVTLRPSGAEHPLPPLGFCLAEGGEALTAPEI
                     GRISALAPGFLRAEVRFSREGWADRLAAAAETARDAGTSLQAALHLGADPAADLDAFG
                     ARAAGLPLASVLVFKDGEKSTSGRWIAEARARLRNAGISAPIGSGTDAFFAELNRGRP
                     PVADLDFLSFSVNPQVHAFDDASLVETIPAQAAAVESARAFAGGRGVTVSPVTLRMRF
                     NPNATGPEPEPKPGEPPANADPRQKTRFGAGWTAASVASLTAAGTASIAYYQCSGPLG
                     LMESGGGAVFPLYQVFADLAEFRGGTLESLESEDPLRVSGYILRKDGRSSLFLVNHCG
                     ESAEAVLEGPAGTFRTRGGSRIQSGGLPLEPEAYLRLDRE"
     gene            11160..12002
                     /locus_tag="P5313_03790"
     CDS             11160..12002
                     /locus_tag="P5313_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRY79520.1"
                     /translation="MKKIALMGAGGKMGCRITDNLKGKPGFEIAYVEVGKEGIERLRA
                     KGVTTTPQDQAVADADYVILAVPDRLIGRISGDIVPKLKGGCKVIGLDPAAAYAGVLP
                     PRKDVTYFVTHPCHPHMFNDETDPKAQKDWFGGIAKQDIVCALYQGPDADYRECEELA
                     KIMYMPVAKSFRITVEQMAILEPAVVETLGLCLVGALKEAYDEAVRMGVPAEAAKSFV
                     LGHARIEFAILFGYADFQVSDGAKLAMEKARDVIFKPDWKKNVMDIARLRESVADITG
                     SLNK"
     gene            12088..12897
                     /locus_tag="P5313_03795"
     CDS             12088..12897
                     /locus_tag="P5313_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRY79521.1"
                     /translation="MKLGISSYAYAWTIGVPGFPGGGMDAFGFLRRCADKGVRAVQIA
                     DNLPVHGMDAARRRELRSLAEGLGVEIQLGMRGCAVPDLRAYLALCEEFGSPLLRVVL
                     DTRTDRPDAAEAVRRLREVARDFERAGVDLSVENHDRFRARDLAALVRGAESSAVGIC
                     LDSVNSFGAAEGTQTVLEELIPLTTCLHVKDFTIARHPHNMGFSLTGVPAGRGFLDVP
                     DILARLATAGRDPDAFLELWPSPEQTPAETEAKEEAWVGESAAYLRTLIRD"
     gene            complement(12955..13653)
                     /locus_tag="P5313_03800"
     CDS             complement(12955..13653)
                     /locus_tag="P5313_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY79522.1"
                     /translation="MDPDLFRTPVRKSTVDTVIDRIKDLLISHKLKPGDRLPNEMDLT
                     KSLSASRGSIREAMKVLSSFGVLEIRRGDGTYVSDSTGQKVFDHLLFQVLLSRIDKSQ
                     LLQLRRLLELGIAQIIIKSASDEQIGHIRKVHEQMGRSLDRDEKDPGILTELDLDFHR
                     AFAEATGNVLIERIYGFTLELFEPSIRKTHENETRLAYTLHTKILEGLAARDEERAAE
                     AILRSIEQWNALSE"
     gene            13966..14937
                     /locus_tag="P5313_03805"
     CDS             13966..14937
                     /locus_tag="P5313_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HRY79523.1"
                     /translation="MILSLGLFLVGLVFLFLGSELVTRQMGPVARRLGINELVVTVLG
                     VSVLSSLPELMVSGFAAARGQSDVSIGNVVGSNFVTLTFVTAVCALIRPMDTSLQIKD
                     RESSWMILSTALVLVLGHDGILGRVDGALLILFYVPYIGSVIAGALREAKSGAGTGQG
                     VAAGGPPLALRLALMAAGLAAIILGADWAVSGGERLGVAAGIPPFALGVVLFAFGTSL
                     PELSIAVSATLKGKSEVSIGEVYASNTFTMMVVLGICALIRPLAVSEAITSFALPFLV
                     LASVVIQVFITTGMRLKRWEAAVLLVFYGFFVFANFTDVLGWTRALP"
     gene            15043..17247
                     /locus_tag="P5313_03810"
     CDS             15043..17247
                     /locus_tag="P5313_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79524.1"
                     /translation="MKGKMERAGTGTGPAESHRWRFARSGGVDQVVFRDGQDIARLRE
                     LDLKLWTALSMPVRGVDLDPRTAALLDVDGDGRIRPQEILAAVDWAVSAFKDLGELMT
                     PGDSVPLASIRDPGILDAARLLLAYLDKPGADRVSMADVADRETSFAGTLFNGDGVVP
                     PEAAGDEPTSRAVRDILAVSGSAPDLGGKPGINAAIAESFFAQADAWLAWRRKGEDPS
                     VAPLGPDASAAAWAALEAVRDKVDDYFTRCRLAAYDPRGAAAVNRDEADFKAVAGGLL
                     SPSAEAIAAFPLAPAAAGKDLPLGGAVNPAWASAVSNLARLAVEPVLGGDRTALSEAD
                     WSALRARFEAYAAWKAEEPGTPASALGAARLAELTAGGFRDRIAELIARDAALAGEAA
                     NIALVEKLVRFRRDLFRLLTNYVNFSAFYRHQGAIFQAGTLYLDARACTLCVEVLDES
                     RQAALAGLSGAFLAYCDLSRPGGLRKKIVAAFTDGDSDNLMAGRNGVFIDREGRDWDA
                     TVTRLVSNPISVREAFWLPYKKLARMIEEQVAKRAQAGESKATAQLAETAGTVANADK
                     AAAEHKPAEPTKKLDLGTIALLGTAIGGVSALVAGFLQTLFGLGVFLPLGVLGILLLI
                     SGPSMILASLKLRKRNLAPILDANGWAINTQARINIPFGASLTSLAVLPPGTLPSLPD
                     PFSEKKKPWGLYLLLLVLAVAAAAGTAWRFGWADRWLPEGARYSAVIGGERP"
     gene            17374..19230
                     /locus_tag="P5313_03815"
     CDS             17374..19230
                     /locus_tag="P5313_03815"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRY79525.1"
                     /translation="MDTPAYVRHEGLKAWVAQIAALCKPDTIHWCDGSREEHDKVCAE
                     LVASGTFIPLNPGKRPGSFLARSDPGDVARVEDRTFICSRRKVEAGPTNNWKDPKEMK
                     DILRGLFDGCMRGRTMYVIPFSMGPLGSPLSHIGVEITDSGYVVANMRIMTRMGKAAL
                     EVLGDHGEFVPCVHSVGAPLAPGQKDIPWPCDKDRKYIVHFPETREIWSYGSGYGGNA
                     LLGKKCFALRIASVIARDQGWLAEHMLILGVESPEGEKTYVAAAFPSACGKTNFAMLI
                     PPAAFEGWKVTTVGDDIAWLKPDAQGQVRAINPEAGYFGVAPGTSEKSNPNAMAAIRA
                     NTIFTNVALTPDGDVWWEGMTKTPPEGLIDWQGMPWDPASGKPAAHPNARFTAPARQC
                     PSIDPDWENPAGVPIKAFVFGGRRSTLFPLVYQSFNWMYGVYLAATLGSETTAAASSA
                     VGQIRRDPFAMLPFCGYHMAAYFNYWLRFGRMIKNPPRIFGVNWFRKDKNGDFLWPGF
                     GENIRVLKWIVERANNHAKGVESPLGWMPRYEDICWKGLEDFGEARFKELMAVDRDQW
                     VGELLSHEELFTKLYDRLPKELIFIRELTLSAIWRSPEHWEFNQTPLEPTED"
     gene            19233..20273
                     /locus_tag="P5313_03820"
     CDS             19233..20273
                     /locus_tag="P5313_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY79526.1"
                     /translation="MPNAPLPEYAAALAAAGAALLAAGVLHRIRLGRLRESLRRRESE
                     LVELSERLRRHSEHLRLVIDTVPAYIYAKDDAGHFILVNRLFARVFGVEPEEVTGKTN
                     EDYGATPEETARFMEEDREILRSGKPLFVPEERAPRPDGSPGWFQTTKIPYRHPDWEK
                     PAILGVSVDITENKEQAEALRRSARHDPLTGLANRALFSELLGKALAVTRRERNKLAL
                     LFIDLDDFKPVNDNYGHAAGDAVLEETARRIERTLRESDGAGRFGGDEFVAFAFGISR
                     REDAAAVAEKVREAIRAPIDLGTSVLGVTASVGFAVFPEDGTDEESLVRRADEEMYRE
                     KQERSGKKRPRT"
     gene            20370..21086
                     /locus_tag="P5313_03825"
     CDS             20370..21086
                     /locus_tag="P5313_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY79527.1"
                     /translation="MFARRARLFLMLGLAAAGLGAQTLSLVTDDVYPRSTPDGRGFED
                     LILKEAFRRVGLAVRITPLPSERALINVNQGIDDGLFVKVAGVEAGYANLVMVPESVC
                     DYEFAAFGRDPTLRITGYPSLAPYSIAFIRGWKNPEAATAGMRSVTRVKDDEALFEML
                     LHGRVDVIVYEALQGRVWIRNHGLTGIHQLGPPLATTEMFLYLNVRHRDLAPRLAAAL
                     KAMKADGTWRGIVDSVVGGL"
     gene            21091..22440
                     /locus_tag="P5313_03830"
     CDS             21091..22440
                     /locus_tag="P5313_03830"
                     /inference="COORDINATES: protein motif:HMM:NF028458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY79528.1"
                     /translation="MAGGIGPRAFRRMLLVCLLLVAGTTAVQYVRRYRSRTESAIEAA
                     ENLRSVQTPALLSALWSSDTDQVRILVDGMALFPYVNYVSVADQDGRVYFAGGMKPSS
                     RPGFFPLVYPYGGREIPVGTLALEIDTERLASDVRSEVLQSLGAQAFLLVLASLLVFL
                     LFERMVTRHLVRIVEHLDGFSPGKVRAPLRLDKKPKGDELEILADSFNALERGVAEAR
                     ADEVRAMEDLRESEEAVRRSLREKETLLQEVYHRTKNNMQVIASLLEMQALISEDEGT
                     RGVMREMVGRIRSMALVHKKLYESKDLSRIDLGEYVEDLTAGIRNGFLDDRKGIEIRV
                     DADQGIVSLLDAAIPCGLALNELVVNAVKHAFPGGRGGLIRVRLGRRDDGMIEMRVED
                     DGIGLPPGFDAERDGRLGLRTVVSLIEYQLHGSVEYRTGEGTTWILAFRDGLFEARV"
     gene            complement(22571..22990)
                     /locus_tag="P5313_03835"
     CDS             complement(22571..22990)
                     /locus_tag="P5313_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79529.1"
                     /translation="MDPTSAPEAILCVDDEGIILLHLVMLLKRRYGDRYRYERASDAA
                     EGLAVVERLESEGARLSILISDWLMPGISGDEFVRSLHETRPKLRSILVSGKSDEDEI
                     RRLCEEAGLAGFLSKPISSEELFALIDRIEEDRRAGT"
     gene            23335..24525
                     /locus_tag="P5313_03840"
     CDS             23335..24525
                     /locus_tag="P5313_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79530.1"
                     /translation="MPMVERRYAPIYAFLVCLFALFGTSMTVIGATLPRILENFGWSY
                     GTAGAVIAANAIGYFGSVFAAGRIVGRIGPRTAALIGLSLVSGGLALFAASPGPALNL
                     ALYALIGIGQGFLEILVNWSVMRMDREGRAMSIMHAAFSIGAVAGPFAVGWLLASGRP
                     WVLIYRGMALLFGVVLAAGLALPFDRLGRETVAARTGGGPSVRDRAYVLGFAVLFLYV
                     GAELGISNWAAELFVRSFRGSEATGSFMVSLFWLGLLAGRLGIPLLLPRARQEKVLVV
                     LSAGLAASTLLLAASSFFGPPAVFVGAASVALAGLFCSSIYPIVIFLEGRAHPGAEGA
                     AIGFAGAGGGLGAFLFPFLMSRIAGAAGVNVGFAFYALVAALVSALALALAATTRGGV
                     PDRG"
     gene            24629..25333
                     /locus_tag="P5313_03845"
     CDS             24629..25333
                     /locus_tag="P5313_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRY79531.1"
                     /translation="MSDSMTRTRFGDLTLERNILRNSFVWMTLGLAFTGIVAWGVSNS
                     PGLLRTLVLNRFLFYGLVFGQLGAVWFLASRVLKMSPAAAAATFVGYSGLTGVTLSVI
                     FLVYTSGSIASVFFITAGMFASLAFLGYVTKIDLTRMGSYLAMGVIGLVIASVVNIFL
                     RSDGLGWIVSLAGVAIFSGLTAYDVQKIKALSARFGSEVDEGNYLRLSIYGALQLYLD
                     FINLFLHLLRLTGRRR"
     gene            complement(25415..26134)
                     /locus_tag="P5313_03850"
     CDS             complement(25415..26134)
                     /locus_tag="P5313_03850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRY79532.1"
                     /translation="MKTFQETTILVEDLEVTLRRRPVRTLRVVIRPPDGRVLASAPLL
                     MPETAIRAFLSEKLPWIRRHRGAVRARERPSSDLESGDTVSWLGRAYRLSVRERGRGI
                     GARLAGPDTLELFVPPGAGPEGRRAILDGWYRERLKELIPPLGAKWETALGVRAAEWG
                     IKRMKTRWGTCNIRARRVWLNLELATRPPECLEYVIAHELAHLIERNHNARFHAILDR
                     VLPDWRERREDLRRNPPARDG"
     gene            complement(26131..26787)
                     /locus_tag="P5313_03855"
     CDS             complement(26131..26787)
                     /locus_tag="P5313_03855"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79533.1"
                     /translation="MERWSRNGTQGGRKPSEEKRESILEAAIRVFSRGGFEASSMDLV
                     ALEAGASKRTVYNYFPSKEILLHEIAARFGREMRAFKRFEYDPAVPIETQLGRFADAE
                     LEVVRNERWMGMIRLLVAAFVRSPELARASVSRDPASEDPLADWMRAAARAGKLDAPE
                     PAMAAAVFRALLGGAYTWPAVYGGGTVPDGGEALKRELVETFLARYRPSDHPAERRAR
                     "
     gene            26896..27627
                     /locus_tag="P5313_03860"
     CDS             26896..27627
                     /locus_tag="P5313_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HRY79534.1"
                     /translation="MDYDSKAARRAGLFYLAAAVFYIFGMMYVEGRVAGDDPELLRKI
                     AETGGLYRLGFLSALLGHVCLLATAAALYELFSPVDAAWARLLVVFVVAGVSVAFLNR
                     AHQLVVERLAVSAYPGLPGTGAEWLAPLFIGLYRDGERLACMFWGLWLLPAGVLAFKS
                     GFAPKALGVVLIAACAFYVLRFFEYFLLDPPPAALRAPLAVANAADSAAKVAAELGFI
                     GWLLAAGFRRRLPADRRESASAARP"
     gene            complement(27610..28395)
                     /locus_tag="P5313_03865"
     CDS             complement(27610..28395)
                     /locus_tag="P5313_03865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRY79535.1"
                     /translation="MWRFIAIAYLTFWAVILGLGGFASMVLGAPEPVMRGIVVLGSWS
                     PTLVLLALRRRFLPGGSVRAFYAKVFAGRIRPGMVLGIPALVFGLFAAAAAAAAVLGG
                     SRPGAGISIEPGALLGTAALMLFLGPSGEESAWRGYLRPELESRYGFLKGNLLLGLVW
                     AFWHAPLWVLASEFRGLEGLVFAGANVVVLTSLTVILGVLMKRSDNLLVAFWTHYCFN
                     VSLSLWSGGIGLFAALSVAYPAAAAILAAVFFRTRSESLGARG"
     gene            complement(28421..>28800)
                     /locus_tag="P5313_03870"
     CDS             complement(28421..>28800)
                     /locus_tag="P5313_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79536.1"
                     /translation="ALLPRAAAFGGELVVAGDGKSDYQIVVPDKVGSPAARHLEAAAV
                     FLQRCLSDAAGARLPVAQESSADPQKPSIFIGAVAVAARAGRVLFLRDGRIDRELELG
                     PPDGQGLEERTGRVSRTMRELGI"
BASE COUNT         4525 a         9839 c         9689 g         4747 t
ORIGIN      
        1 ggatagttcc gctccgtcat gatgtcgtac atcttccgcg cccccgccag gcccgcctgg
       61 ttgaggacgt cccggtcgat cgggatgccc tgggccttga cctggttctg gaggtggtcg
      121 tcgtagatcc ccgcgatgta ggaaaggtag agggggggct tcctcccggt tttccgggag
      181 acgcggttgt aggtctcgca gagcgccacc ccctgggcca cggacatgat ctcggtggcg
      241 ttgatcggtg tgtcctcggc gatcaggacc tccatggcct ccaggcccgc ctcggtggtg
      301 gggatcttga tgcagatgtt cggggagacc ttccggttct cccgggcctc gtcgatgatc
      361 acctcggggt cgtcctcgtg gaccggatcc ccctggatgc tcacccaccc gtccgcgccg
      421 tgggaggcct tccaaatcgg gaggaaggct tccgccacgg gggccacgag ccggcgctgg
      481 aagatcgccg cggcctcgtc gtcgctcctg gaaacccgga tcgattcgtc caggatcttg
      541 ccggcgtagg gtccctcctc gggatgggcc agcatcttcc aggtgtacga gggattgttg
      601 gtgcagccca gggccccggc ggcgatggcc gtggcggcct cggcccgggt cgggttgttg
      661 atccagaact tggtgggggt cagccggtgc acccggtgga aatagtcctt gctcatgaag
      721 aatcctccag aaaaaattac cgcgagctga ccggggaacc ggccggaccc gccgctcact
      781 cgacggcgag ccgctcccgc ggagcctgca atatgtcccg gaaggccgcc agccagcggc
      841 ctcccagggc cccgtccacg gcccggtggt cgacggagag ggtggcggtc aggacgggcc
      901 ggaccgtcaa gaccccgtcc cgggccacgg cctcctccgt cacggccccc acggccagga
      961 tacccagctc cggggggttg atgaccgccg cgaactgccc caccccgaac atcccgaggt
     1021 tggagaccgt gaaggtcccg tcggaatact ccgagagctt gagcttcccg agccgggccc
     1081 gtccggccag gtcccgggcc ttgtcggcca actcgaccag ggtcaggcgc tccgccccgg
     1141 ccaggaccgg gacgatgagg cccttctcca ggctgaccgc catgcccagg ttcacggtgt
     1201 cgtagaggga gagggtctcc ccctccaggc gcccgttcaa cccggggacc tcgaccaggg
     1261 cccgggccgc ggcggacagg gccaggtcgt tgtaggagac cttccggtcc cgggcggcca
     1321 ggtcgcggcg caggtccgcg gccgcgccca tatccacccg catcgagaag taggcgtggg
     1381 gggcttcccg cttgctccgg gacaggcggt cggcgatcac cttccgcagg cccgacaggg
     1441 gctccgagct tcggggcgag agcatcggct cagtcctccg tgtcgatctc ggcgatctcg
     1501 gagaagagct tgaccgtgtc gccctgggcg accaggaggc gggtgatctt cccggagtac
     1561 tccgactcga cctcgaccgt ggtcttctcg gtctccgcgg agaagagagg atccccggcc
     1621 ttcaccgagt cgccgggctt gacgaaccag gtatagagga ccacctcctc catctcctgg
     1681 ctgaccttgg gcatgctgat catcttcagg gccatcgtgc ttccttcctt gggttcgaga
     1741 ttgggcgggg ccgccgacgg ccgcccgccg ggcggaccgg cccgcgggcc ggcgccggct
     1801 tcaccacatg acctggccgc cgtccacgtt gatcgcctgg ccggtgatgt gggaggccag
     1861 gtcggagatc aggaaggcgg tcagacgggc ctggtcctcg ggctccgcga gccgtcccat
     1921 cgggatgccc gccagccact tggccagggc ttccgggtcc ccgccggtga tggtcttcag
     1981 gaccatgtcc gtggcggtgg tgcccgggca ctgggcgatc acccggaccc cgtgaggggc
     2041 cagctcgaag gcggccacct tggagaagtg gatgaccgag gccttggccg cgcagtaagc
     2101 ggccatgttg agcctctgga tcttgccggc cacggagcct atgttcacga tcacaccccg
     2161 gcgccgctcg atcagccggg gggcgatcgc cttgcagcag aggaagaggc ccttcacgtc
     2221 cacggagaag atggcctccc agtccttgag gtcgatggtg tggaagggcc gtggcgggat
     2281 caggatggcc gcgctgttca ccagggcgta caggggcccc agctccttct ccgcggcgtc
     2341 catcgcggct ccgacggccc tctcgtccgt cacgtccagc ttgtacgccc gggccttgcc
     2401 gccggcggcg gtcaccgcgg ccgcggtctc ccgggccccc gcctcgtcca ggtccgcgac
     2461 ggccacggga gccccgcact ccgccaggac cttggcgatc gcccggccta tgcccttggc
     2521 cgccccggtc acgaagacgg gtcgtccctt caggtcgatg tccatgacgt tctcccttcc
     2581 gcggcctagg ccttgcgctc gtacccgacc aggtcccggg ccgcgacgac gacctgctcg
     2641 gcggtcggca cggcggcggc gcagaggttg ggggcgtagg cgatgtgggt gtggaggccc
     2701 ccgatgcgcc gtaccggcgc ccggaggtcc cggaagagct cctgctggat cagggtgacg
     2761 atctcgcccc ccgccccgta gtgaaccggg gcctcgtaga ggaccatggc ccgcttggtc
     2821 ttggccaccg aggccaggac cgcctcccgg tccaggggca tgagacagcg caggtccagg
     2881 acctccgcct cgatcccttc cttctggagg agctcggcgg ccttgagggc cgtgtgcacc
     2941 gtgatggagt agcagacgat ggtcaggtcc ttgcccggcc gcttgatctc ggcctttccc
     3001 agggggatcg agtactcccc gtccgggacc tcgcccttgg tgaagtacag ctgcttgtgc
     3061 tccaggaaca ccacggggtt gtcgtcccgg atggcggaaa ccatgagccc cttggcgtcc
     3121 gcgggggtcg ccgggaggac caccttgagg ccggggacgt gggcgaacca ggcctccagg
     3181 ctgttcgtgt ggtgggatcc gcggcccgga ccggcaccct ggggacagcg caggaccatg
     3241 gggcagttca cccggcccgc ggagttgaac caggacttgg aggcctggtt gagcagggcg
     3301 tccaggccga aggtcgtgaa gtccacgtac atgatctcgg ccacgggccg caggcccgcc
     3361 atggccgatc cgacggcggc gttgatgatg atctcctccg ccaggggggt gtcccggacc
     3421 cgttcctccc cgaactccgc caggaggccc tccaggacct tgaagatgtt ccccgtggtg
     3481 cccacgttct cgcccatcac gaagacgtcg gggtcccggc gcatctcctc cgcgatggcc
     3541 cggcctatgg cttttccgta ggtaagctcc gccatctcac tgctccttcc agacgtactt
     3601 ctcgaggtat tcctcgccgg ggtaggggct ggcgtccgcg aaggccaccg cttccgcgag
     3661 ttccttcttg atcgcgtcga ccatcgcctt gtccttctcc tccgtgagca ggcccttgga
     3721 aatgaggagg gccttgcacc gcttcacggg atcccggttc tgctcccagt ccttcacgtc
     3781 ctcccgggtg cggtagtacc actgggggtc tccctcgttg tggccccggg tccggtaggt
     3841 cttacactcc aggaaggtcg ggccctcgcc ggcccgggcc cgccggatgg cggtgctggc
     3901 ggcctcgtag accgcgacga ggtcgttccc gtccacgacg atcccgggca gggagaagcc
     3961 gtccgccctc cgcgccacgt cggcgatgga gatcatcttg gtcgcggggc tggagatggc
     4021 gaaaccgttg ttctccagga cgaagatcac ggggagcttc cagatggagg ccaggttcat
     4081 cccctcgtag aggaccccgt ggctggacgc cccgtccccg aagaaggcca ccgcgacccc
     4141 gccgtccttc cggtacctgt gggccaggcc ggcgccgacc gcgagtccta tgccgccgcc
     4201 caccactccg ttggagtgga ccaggccgat ctcgggcacg gacatgtgca tgaccccgcc
     4261 cagacccttg ccgtagccgt ccacccggcc gaagctctcg gccatcatcc ggtcgaaccg
     4321 ggcgcccatg gacacgaggt gcccctcggc ccggtgggtc gagaagaccg agtccttggg
     4381 gttcaggtcc gcgcagatcc ccgcggcgat cgcctcctcg ccggtgtaac aatgcaagta
     4441 tccgtggatc ttgcccttgg agttctgggt gcattgctcg agcacgaagt accgttcccg
     4501 gagcatgacc tcgtactgcc taagcagttt cccgtcttcc acttcaaggc ctccttccgc
     4561 ctagttccgt acaggttttt ccgtggccac caccaggacc aggatgatca ggacgccctt
     4621 cacgatcttc tggacgcccg cgtcgaatcc cgccacctgc atggccgtga cgatgatcac
     4681 gaagaagagg cacccgaaca gggtacccag gaccgtggac cttcccccga agatgagggt
     4741 cccgccgatc accaccgctc cgatggtctc catgaggtag gaatccccca tccccaggaa
     4801 ggccccggag acccgggccg ccaggagcac gcccatggcc ccggagaaga gccccgagat
     4861 cacgtaggcc gccagctgga cgcgccggac ccggatgccc gagagggccg cggcctccac
     4921 gttctggccc accgcggtca gggaccgtcc gtacacggtg cgcttcatga gccagtaggc
     4981 cgcgagggcc aggaggcccg tgaccaggaa catgaagggc acccccagga agcggccccg
     5041 ggtcagggtg accaggaggg gggcgctgtt gaaggtccgg aacccctcgt tgtagatcag
     5101 ggacgccgag gtcaggacgt agcccgtggc cagggtggcg atgatgggag gaatctccag
     5161 gaagaggatg atgagcccgt tggccaggcc caccagggcg cccgcggcca gggagaccgc
     5221 cacgccttgg aggatgttcg cgtcctgggc gttgatgatc cccgtggaca ggaaggccgt
     5281 gatcgtgatg actcccggta tggagaggtc gatggcgcct cgaccgcagg agatcacgta
     5341 catctggccg atggccgcga tggccaggaa ggccgcggac atggaattgc tgaccaggct
     5401 ctccaggttg agccgggagg acacgaggcc catcccgagc cagagcagga aggaaccggc
     5461 ggcggcccag atccaacgct ggacctccgc gaaggcccga aaccgggcgg cgatcttgtt
     5521 catacgctcc tccgccttcc gacgagccgc agggccagga cggcgatcag gatggccccc
     5581 tgcaccgccg ccacgaggtt cgtgctcagg ttcaggaagc ccaggagggc cccgatcagg
     5641 gacagggtga tcgccccgaa gaccgcgccc aggggcgaca cgatgccccc ggtcaggtcc
     5701 ccgcccccca tgaccacggc ggccacggtc agcagggtgt aggagagggt ggcgttggcg
     5761 tcggccccgg tggtgacggc ggtcacgaag atccctccca ggagcccgaa gacgccggac
     5821 aggaagtagc ccgccatgta ggccttcagc accgaccacc cggagcgctc cagggcggcc
     5881 gcgttgttgc cgaagccccg gagcaccgtg ccgtaccggg accggtgaag gaggaccgcg
     5941 gcggcggtca cgactcccac gatgatcagg acgaagggga tcacggggaa ctggaaaccg
     6001 tagaaggcga tcagccagtc cggggccttg cccccgggca tgctcaggat ggtataggcg
     6061 atgcccgtcc agatgaagga cgcccccagg gtgacgatga tggcggggat ctgccgcagg
     6121 tggatcaggg cgcccagtcc cgcgtaggcc aggagcatga ccgcgtagca ggccacggcc
     6181 gcggcggggt tcgtctccag cagggtcgca ccgatcacgt tgaccagtcc catgaaggcg
     6241 cccacgctca ggtcgatcat ggacaggccg atgacccaca tctgggccag ggtggccagg
     6301 atcaggggga tggatcctcc gatcagcagg gtgatcccga attcggaaaa gaccgcggga
     6361 gtcaggatgc cggaaacgat gtaaaccagg agcatggccc cgagggggat cccgatcccg
     6421 gagacgtcga acttggagac cttggctatg gaggcggcct cgggcttcag gtgctctccc
     6481 gagaaatagg cgttcaccac gctgttcttg tcgatctcac cccgggaaag tttcttgacc
     6541 aaggtgccgt accgcaggat gtggacctcg tcgcagatct cgaactcctc gtcctcggtg
     6601 ctgtaccaga ggatgagctt gcccttgtcc gcggcttccc ggaaaatctc gtagagctgg
     6661 agcttggtct ggatgtccac gccccgggtc gggtcgtcca ggatcacgat gtccgcgtcc
     6721 gagatcatgg cccgggcgat caggaccttc tgctggttcc cgcccgacaa actagtgatc
     6781 ggggctcccg gtccggcggc gaccaccttg agccggtcgt accaggaccg cacggattcc
     6841 tgccccgtgg tccgtcccac cgggcggatc ccgcccagac gggccaggcg ggagatgacc
     6901 gcgttctccg cgatggacca gagggggaag agcccctcct tcttacggtc cccggtcacg
     6961 taggccaccc ggcctgtccg ctccaccgag cgggccgcgg agcccgaggg ggcgaacacg
     7021 gcgcgcagga gttccctctg accggagccc tccaggcccc cgatgcccac gatggagccg
     7081 ccccggaggt cgaggtcgat gtcccgcagg gcgtgggtcc ggagcccccg gatggtcagc
     7141 cgggccgagc actccccggc ggatcccgcc ccgggctcga cgcaggcccg ggatccgggc
     7201 acggaccgtt cccggaggac agcctctccc atgcggcgaa ccaggtcctc ctcgtcggtg
     7261 tccgccaccg ccccggacca cttgacctgg ccgttctgca tcaccaccac ccggtccacc
     7321 agggtcatga cctcgttcag gcggtgggag atgaatatga aggaaacccc ctgggcgcgg
     7381 cgattccaga tgtagttctt cagctgttcc gtctgctcca cccccaggga ggaggtgggc
     7441 tcgtccagga tcagtatgcg aaggccggga tcgcttacag agcgggcgat ctcgaccatc
     7501 tgtttctgcg ctatggtcag ggcgctcaag gattggcgct cgtcgatccc gttgcccggg
     7561 aagacggcgt ccaggctggc tcgggcggcc ttgcgggctt cctcccgcca tttgcggaag
     7621 gggccgcggt accgggccgc gtgctccacg tagaagttct cgtagaccgt caggttcgtg
     7681 gagagggaaa gctcctggta cacaacccgg attccgagcc ccgcggcccg ggcgggggag
     7741 tactcatccc accggatcgt cgaacccccg acctcgaggg tacccgaatc gggccgggtc
     7801 accccggaca ggacccgcat caaggtgctc ttccccgccc cgttggcccc cacgaggccg
     7861 atcacctcgc cggccgagat ggagagggac acgtcctgga cggccttggt ggaaacgtag
     7921 gtcttggaca agccttcggc ggagaggagg cggacgcgga cggccggatc ctcccgggca
     7981 gtcatggatt ccattcggac ctcatgggac cctccccggg cggaaggccc ggggagggtg
     8041 gatgatcggt tacggcttgg gggccgtacg tacgaggggg atcacttcgc ctttatgatc
     8101 ttctccatca cgtagtccca ggtgaaggtg ggggacgcga gggtgccggg cttcagggtg
     8161 gagaaatcct tggcgttggc gttggtgatg gggatgacct tgaggctcat cttcttgggc
     8221 acgtcgacct tgttgaggat cgcgagggcg gtccagaagg cggcgccgcc gatgccgggg
     8281 gcggagcaga tggagtaggt gctgtacccg tcgttcatct tcttcatcca ccactggatg
     8341 aattcggcgg agttgtcccc gatgatgatg ggcatggggc ggttggcggc ctcgaaggcc
     8401 tggaccgcgc cgtaggcgtc gccgccgccc tgggtcagga ccgcgtccac ctggggcagg
     8461 ctgggcagga tgttggcgac cgcggcttgg gtgaccgtgg tggtcgcttc gccgtatacc
     8521 gtaccgacga ccttgaggtt gggatacttg gccaggacgt tcatctggcc cttgtacatg
     8581 tcctggtccg gagcggagcc ggtcacgccg cggacgatga gcacgttgcc cttgccgccg
     8641 agcttgtccg ccacgaactg ggcgccgccc tctccctgct ccacgaaatt gaagtccatg
     8701 gtccaggcgc tgggggtgga gacgatggag tcgaaggcca ggaccttgac gcccgccttg
     8761 gcggcctggt cgatcacgcc gttcagggcg gtcggggagg cggcgttgat gcagatcgcg
     8821 tcgacctttt tcaggatcag ggagttcatc tgggagatct gggtgttctg ggtcccgtcg
     8881 ccgttgacga tgacatagtc cttgatccaa ccctgggcct tggcggcctt ggcggcttcc
     8941 tcgaaggcct cgacgctctg cttgcgccac atattgccgt agtaggagtt ggagagggcg
     9001 atcacccact tgcccttctt gggctgctgg gcggccgcgg acatggaccc gaacgccatc
     9061 aggatcgcga gggcaatgag cactgtcttt ctcaacgggg gcctccttgc tggattgtaa
     9121 acattagaca tctaatgtct tagatcagag gctagcacca gtttctatag ctgtcaagag
     9181 agaaattcaa cgcgtcccga aaatcccggt tcagtctttc gttgacgccc gatgtaaagc
     9241 atggtagagt tgttcaaaca ttatacgtct tacgtatgac agcaaataca ggaggaaagg
     9301 atgcagaaac ccaatccgtc gaagcccgca gcccccggac tgtcctccga tcgtctccgg
     9361 cttgcggccg ggcccgtctc cgcggtcttc gaggacggat tcctccgata tattcgcttg
     9421 ggttcgacgg agatcgtgcg gggcctttac tccgcggtcc gggaccacaa ctgggacacc
     9481 attcccggcc ggatccggga cttccggatg gagcgcgggg aggattcctt cacccttacc
     9541 tacgtctcgg agcaccggcg gggcagtatc ggattcacct ggaacgccga aataaccgga
     9601 actcccgacg gcaccctctc ctattcgatg gacggcaccg tcctcaccgc cttccggaag
     9661 aaccgcatcg gcttctgcct cctccacccc atgtccctgg ccggactacc ctgccgggta
     9721 acccactccg acggctcccg ggaggacggg gcctttccgt ccctcatctc cccgcaccag
     9781 cccttcctgg acatgaggcg gatcgagtac gaggtcggag gcatacgggt ttcccaggac
     9841 ttcgaggggg agatcttcga gatggaggac cagcgtaact ggacggacgc ctcctacaag
     9901 acctactgca ctcccctggg ccttcccttc cccgtcgagg tgcgggaggg actgaggatc
     9961 cgccagcgga tcgtgatccg ggcggcccgt accgaggggt ctcccgtccg ggtcgaggag
    10021 cccgggaccg cctccgtcac cctgaggccc tcgggcgcgg aacatcctct gccccccctg
    10081 gggttctgcc tggccgaggg cggcgaggcc ctgaccgcgc ccgagatcgg gcggatctcc
    10141 gccctcgcgc ccggtttcct ccgagccgag gtccggttct cccgggaggg ttgggcggat
    10201 cgcctggcgg ccgcggccga aacggcccgg gacgcgggta cctccctgca ggcggccctg
    10261 cacctgggcg ccgatcccgc ggcggacctg gatgccttcg gcgcccgggc cgcgggcctg
    10321 cccctggctt cggtcctggt cttcaaggac ggtgaaaagt ccacctcggg ccgatggatc
    10381 gcggaggccc gggcgcggct ccggaacgcg ggaatcagcg ccccgatcgg gtccgggacg
    10441 gacgccttct tcgcggagtt gaatcgcgga cgcccccccg tggcggacct ggacttcctg
    10501 tccttctccg tcaaccccca ggtacacgcc ttcgacgacg cctccctggt ggagacgatc
    10561 cccgcccagg ccgccgcggt ggagagcgcc cgggccttcg ccggaggccg aggggtgacc
    10621 gtaagccccg tgaccctccg aatgcggttc aaccccaacg ccacgggccc ggaaccggaa
    10681 cccaaacccg gtgagccgcc cgccaacgcg gacccccgac aaaaaacccg tttcggggcg
    10741 ggctggaccg ccgcctcggt ggcatccctg acggcggccg gaacggcctc tatcgcctac
    10801 taccaatgct ccgggcccct ggggctgatg gaatccggag gaggggctgt cttccccctg
    10861 taccaggtct tcgcggacct ggcggagttc cgcggcggga ccctggagtc cctggaatcc
    10921 gaagaccccc tgcgcgtatc ggggtatatc ctccgcaagg acggccgctc ctccctcttc
    10981 ctggtgaacc actgcggaga gtcggcggag gcggtcctgg aaggcccggc ggggaccttc
    11041 cggacccggg gaggatcgag aattcagtca ggcgggctac cgctcgagcc ggaagcctac
    11101 cttcgtttgg acagggaatg aaccggcagg gaccggcaac acatcgcagg aggacagaca
    11161 tgaagaagat cgcgctcatg ggtgcgggcg gcaagatggg ctgccggatc accgacaacc
    11221 tcaagggaaa gccgggtttc gagatcgcct acgtcgaggt gggcaaggag gggatcgagc
    11281 ggctccgcgc caagggtgtg accacgactc cccaggacca ggccgtggcc gacgcggact
    11341 acgtcatcct ggccgtgccg gaccgtctga tcggcaggat cagcggggac atcgttccca
    11401 agctcaaggg cgggtgcaag gtcatcggcc tggatcccgc ggccgcctac gccggggtcc
    11461 tgcccccccg gaaggacgtc acctacttcg tgacccaccc ctgccacccc cacatgttca
    11521 acgacgagac cgaccccaag gcccagaagg actggttcgg cgggatcgcc aagcaggaca
    11581 tcgtctgcgc cctgtaccag ggccccgacg ccgattaccg ggagtgcgag gaactggcca
    11641 agatcatgta catgcccgtg gccaagagct tccgcatcac cgtggaacag atggccatcc
    11701 tggagcccgc cgtggtggag accctggggc tctgcctggt gggggccctg aaggaggcct
    11761 acgacgaggc ggtgcgtatg ggggttcccg cggaggcggc caagtccttc gtcctgggcc
    11821 acgcccggat cgagttcgcc atcctcttcg ggtacgcgga cttccaggtc tccgacggcg
    11881 ccaagctggc catggagaag gcccgggacg tcatcttcaa gcccgactgg aagaagaacg
    11941 tgatggacat cgcccgcctc cgggagagcg tggcggacat caccggcagc ctgaacaagt
    12001 gacggccccg gccgcggcgg gccgcccggg aggcaggccg gcgcctcggg cggggaacgc
    12061 ccgccgcggc cgccgaaagg gatgagcatg aaactgggta tcagctccta cgcctacgcc
    12121 tggactatcg gggttccggg cttcccgggc ggcggcatgg acgccttcgg gttcctccgg
    12181 cgctgcgcgg acaagggcgt ccgagcggtc cagatcgccg acaacctccc ggtccacggg
    12241 atggatgccg cccgccgccg ggagctccga tccctggccg aagggctcgg ggtggagatc
    12301 cagctgggga tgcggggctg cgcggtgccg gacctccggg cctacctcgc cctctgcgag
    12361 gagttcggct cccccctcct tcgggtggtc ctggacacca ggaccgaccg accggacgcg
    12421 gcggaggccg tccggaggct ccgggaagtg gcccgggatt tcgagcgggc gggagtggac
    12481 ctgtccgtgg aaaaccatga ccggttccgg gcccgggacc tggccgccct agtccgcgga
    12541 gccgaaagct ccgccgtggg catctgcctg gattcggtga actccttcgg ggccgcggag
    12601 ggcacccaaa ccgtcctgga ggaactgatc cccctgacca cctgcctgca cgtcaaggat
    12661 ttcacgatcg cccggcatcc ccacaacatg ggcttctccc tgaccggggt ccccgcgggc
    12721 cggggcttcc tggacgtccc ggacatcctt gcgcgcctgg cgacggcggg ccgggatccc
    12781 gacgccttcc tggagctctg gccctccccg gagcaaacgc ccgcggagac cgaggccaag
    12841 gaggaggcgt gggtgggcga gagcgcggcc tacctgcgca ccctgatccg ggactgacgg
    12901 cccggagcgc cgggccgatc ggccggattc tcggggatca gtcccggccg gggttcactc
    12961 ggacagggcg ttccactgct cgatagagcg aaggatcgcc tccgcagccc tctcctcgtc
    13021 ccgggccgcg agaccttcca ggatcttggt gtgcagggta taggcaagcc gggtctcgtt
    13081 ctcgtgggtt ttccgtatgg acggctcgaa gagctccagg gtgaagccgt agatcctctc
    13141 gatcaggacg ttgcccgtgg cctcggcgaa ggcccggtga aaatccaggt ccagctcggt
    13201 caggataccg ggatccttct cgtcgcggtc caggctgcgc cccatctgct cgtgaacctt
    13261 gcggatgtgc ccgatctgct cgtcgctcgc ggatttgatg atgatctggg cgatgccgag
    13321 ctccaggagc ctgcggagct ggagcagctg tgacttgtct atccgggaga gcagaacctg
    13381 gaacagcagg tgatcgaaaa ccttttggcc cgtggagtcg cttacgtagg ttccatcccc
    13441 gcgccggatt tccagtacgc cgaaggagct caggaccttc atcgcctctc gtatggatcc
    13501 gcggctggcg gacaggctct tcgtgaggtc catctcgttc ggaagccggt cgccgggctt
    13561 gagcttatgg gagatcagga ggtccttgat cctgtcgatg accgtatcga ccgtggactt
    13621 ccgtacgggg gttcggaaca ggtcaggatc cacaacatgt acttccgaca taggggctcc
    13681 cgtttatgct atcataagat gtaggatgac aaatatcgga taaatatata acgaatacta
    13741 tcctctgtca acgaaaccgg gcttcacgga ttccccggcc tccgagtcgg tccgtttcga
    13801 tggcgaaacc cgtgcatcca acaggatttt catatgatat ccaatgtcgg acgttggaca
    13861 acattataca ttaaattttg gaacctgtac agaaaaacct cccccccggc acaaattcgc
    13921 acgcccgcgg ccgtcgtact atacttccct gcgacgggag gtgtcatgat cctttccttg
    13981 gggctttttc ttgtcggcct cgtcttcctt ttcctgggaa gcgagcttgt cacacgccag
    14041 atggggcccg tggcccgcag actggggatc aacgagctgg tggtcacggt cttgggcgtc
    14101 agcgtcctct cgagcctgcc ggagctcatg gtctcgggct tcgccgcggc ccggggacag
    14161 agcgacgtga gcatcgggaa cgtggtggga tccaacttcg tcaccctgac cttcgttacc
    14221 gcggtctgcg ccctgatccg gcccatggac acctccctcc agatcaagga ccgcgagtcc
    14281 agctggatga tcctgtccac cgccctggtc ctggtgctcg gacacgacgg gatcctggga
    14341 cgggtggacg gagctctcct gatacttttc tatgttccgt acatcgggtc cgtgatcgcg
    14401 ggggccctgc gggaggccaa atcgggcgcc gggacggggc agggcgtcgc ggccggaggg
    14461 cctcccctcg ccctgcggct ggccctgatg gccgcgggcc tggcggccat catcctcggt
    14521 gcggattggg cggtatccgg tggcgaacgc ctgggtgtcg ccgccggaat cccccccttc
    14581 gccctggggg tggtcctgtt cgccttcggg acgagcctgc cggagctgtc catcgcggtc
    14641 agcgccaccc tcaagggcaa gagcgaggtc agcatcggcg aggtctacgc ctccaacacc
    14701 ttcaccatga tggtggtcct ggggatctgc gccctgatcc gtccactggc ggtcagcgag
    14761 gcgatcacga gcttcgccct gcccttcctg gtcctggcca gcgtggtcat ccaggtcttc
    14821 atcacaacgg ggatgcggct caagcgctgg gaggccgcgg tcctcctggt cttctacggg
    14881 ttcttcgtct tcgccaactt caccgacgta ctgggctgga cccgggctct gccctagacg
    14941 gttccgctcg attgcccggg ggcggagccg ggcctatact ggaagtaccg gcgtaggccg
    15001 gcttcgtccg gaggccggcc ttcgcggggc aaggagcgca ccatgaaggg gaaaatggaa
    15061 cgagccggta cggggacggg gcccgcggag agccaccgct ggcgcttcgc ccgatccgga
    15121 ggcgtggacc aggtggtctt ccgggacgga caggatatcg cgaggcttcg ggagctggat
    15181 ctcaagctct ggaccgccct gtccatgccc gtccgggggg tggatctcga tccccggacc
    15241 gcggccctgc tggacgtcga cggagacggg cggatccgcc cccaggagat cctggccgcc
    15301 gtggactggg ccgtctcggc cttcaaggac ctcggggagc tcatgacccc gggggactcg
    15361 gtgcccctgg cctccatccg ggaccccggg atcctggacg cggcccgcct cctcctggcc
    15421 tacctggaca agcccggcgc cgaccgggtg agcatggcgg acgtggcgga ccgggagacg
    15481 tccttcgcgg gcaccctgtt caacggggac ggagtcgtac ccccggaggc ggcgggcgac
    15541 gaacccacct cccgggccgt tcgagacatc ctggccgtct cgggatccgc cccggacctg
    15601 ggcgggaagc cgggaatcaa cgccgccatc gcggaatcct tcttcgccca ggccgacgcc
    15661 tggctggcct ggaggaggaa gggcgaggat ccctcggtcg cccccctggg cccggacgcc
    15721 agcgccgcgg cctgggccgc cttggaggcc gtgcgcgaca aggtcgacga ttacttcacc
    15781 cgctgccgcc tggccgccta cgacccccgg ggcgccgccg cggtcaaccg cgacgaggcg
    15841 gacttcaagg cggtcgcggg cggcctttta tccccctccg cggaggcgat cgcggccttc
    15901 cccctggccc ccgcggcggc cgggaaggac ctgcccctgg gaggcgctgt gaatccggcc
    15961 tgggcttccg cggtgtcgaa cctggcccgg ctggcggtgg agcccgtcct gggcggggac
    16021 cggaccgcac tgtccgaggc ggactggtcg gcccttcggg cccggttcga ggcctacgcg
    16081 gcctggaagg cggaggagcc cgggacgccc gcctccgccc taggggcggc gcggctcgcg
    16141 gagctgaccg ccgggggctt ccgcgacagg atcgcggaat tgatcgcacg ggacgccgcc
    16201 ctggccgggg aggcggcgaa catcgccctg gtggagaagc tggtgcgctt ccgccgggac
    16261 ctcttccgcc tgctgaccaa ctacgtgaac ttctccgcct tctaccgcca ccagggggcg
    16321 atcttccagg ccggaaccct ctacctggac gcccgggcct gcaccctttg cgttgaggtg
    16381 ctggacgaat cccggcaggc cgccctggcc gggctttcgg gggccttcct ggcttactgc
    16441 gacctgtcgc gccccggggg cctgcggaag aagatcgtcg cggccttcac ggacggcgac
    16501 tccgacaacc tcatggcagg gcgtaacggg gtcttcatcg atcgggaggg acgggactgg
    16561 gacgccacgg tcacgcgcct ggtctccaac cccatcagcg tgcgggaggc cttctggctg
    16621 ccctacaaga agctggcccg gatgatcgag gaacaggtgg ccaagcgggc ccaggccggg
    16681 gaatccaagg ccaccgccca gctggccgag accgccggga ccgtggccaa cgcggacaag
    16741 gcggcggcgg agcacaagcc cgccgaaccg accaagaagc tggacctggg aaccatagcc
    16801 ctgctgggca ccgcgatcgg cggcgtgagc gccctggtgg cgggattcct ccagaccctc
    16861 ttcgggctgg gggttttcct gcccctcggg gtcctgggca tcctgctctt gatctcgggg
    16921 ccgtccatga tcctggcctc tctcaagctg aggaaacgca atctggcccc catcctggac
    16981 gccaacgggt gggccatcaa cacccaggca cggatcaaca tacccttcgg ggcgtccctc
    17041 acgtccctgg ccgtcctgcc tccggggacc ctgccctccc tgccggaccc tttctcggag
    17101 aagaagaaac cctggggcct gtatcttctc ctgctcgtcc tggccgtcgc ggccgcggcg
    17161 ggcacggcct ggaggttcgg ctgggccgac cgttggctgc cggagggggc gcggtattcc
    17221 gcggtcatcg gaggcgagcg gccgtgatcg tcgccgtccg gttcggtcag ggccgggatc
    17281 cggacggcgt tttcttccac cctttttcgg tatttcctta tatactggat tccgtcgcgc
    17341 agcccccgcg cgatttcata cacggaggat tccatggaca cccccgccta cgtgaggcac
    17401 gagggcctga aggcctgggt cgctcagatc gcggccctct gcaagcccga caccatccac
    17461 tggtgcgacg gctcccggga ggagcatgac aaggtctgcg ccgagctcgt cgcctccggc
    17521 accttcatcc ccctgaatcc cgggaagcgg cccggcagtt tcctggcccg gtccgatccg
    17581 ggggacgtag cccgggtgga ggaccggacc ttcatctgct cccgccgcaa ggtggaggcg
    17641 ggccccacga acaactggaa agatcccaag gagatgaagg acatcctccg ggggctcttc
    17701 gacggttgca tgcggggccg gacgatgtac gtcatcccct tcagcatggg tcccctgggt
    17761 tcccccctgt cccacatcgg ggtggagatc accgattccg gctacgtggt ggccaacatg
    17821 cggatcatga ctcggatggg gaaggcggcg ctggaggtcc tgggggacca cggcgagttc
    17881 gtgccctgcg tccactccgt gggggctccc ctcgcgccgg ggcagaagga cattccctgg
    17941 ccctgcgaca aggaccggaa gtacatcgtc catttcccgg aaacccggga gatctggtcc
    18001 tacgggtccg ggtacggcgg caacgccctg ctggggaaga agtgcttcgc cctgcgcatc
    18061 gcctcggtca tagcccggga ccagggctgg ctggcggaac acatgctgat cctgggggtc
    18121 gaatcgccgg agggcgagaa gacctacgtg gccgccgcct tccccagcgc ctgcggaaag
    18181 accaacttcg ccatgctgat cccgcccgcg gccttcgagg gctggaaggt caccaccgtg
    18241 ggggacgaca tcgcctggct caagccggac gcccaggggc aggtccgggc catcaacccc
    18301 gaggccgggt acttcggggt ggctccggga acctcggaga aatccaaccc caacgccatg
    18361 gccgccatcc gggccaacac gatcttcacg aacgtcgccc tgaccccgga cggggacgtc
    18421 tggtgggagg ggatgaccaa gaccccgccg gagggcctca tcgactggca gggcatgccc
    18481 tgggacccgg cctccgggaa gcccgcggcc catcccaacg cccggttcac ggccccggcc
    18541 cggcagtgcc cttccatcga cccggactgg gagaacccgg cgggggtgcc gatcaaggcc
    18601 ttcgtcttcg gcggccgccg ctccaccctc ttccccctgg tctaccagtc cttcaactgg
    18661 atgtacgggg tctacctggc cgccaccctg ggctccgaga ccaccgcggc ggcctccagc
    18721 gcggtgggcc agatccggag ggaccccttc gccatgctgc ctttctgcgg ctaccacatg
    18781 gcggcctact tcaactactg gctgcgcttc ggacggatga tcaagaaccc gccccggatc
    18841 ttcggggtca actggttccg gaaggacaag aacggggact tcctctggcc cggcttcggg
    18901 gagaacatcc gggtgctcaa gtggatcgtg gaacgggcca acaaccacgc caagggcgtc
    18961 gagagccccc tgggatggat gccccggtac gaggacatct gctggaaggg gctggaggac
    19021 ttcggggagg cccggttcaa ggagctcatg gccgtggacc gggaccagtg ggtcggggag
    19081 ctcctctccc acgaggaact cttcaccaag ctctacgacc gcctgcccaa ggagctcatc
    19141 ttcatccggg agctcaccct ctcggccatc tggcgatccc cggagcactg ggaattcaac
    19201 cagactcccc tggaacccac cgaggactag gagtgccgaa cgctcccctg cccgagtacg
    19261 cggcggccct ggccgccgcg ggcgccgccc tcctggccgc aggcgtcctg caccggatcc
    19321 gcctgggccg cctgcgggag agcctgcggc gccgcgagtc ggagctggtg gagctgagcg
    19381 agcggctccg gcggcattcc gagcacctgc ggttggtgat cgacaccgtg cccgcctaca
    19441 tctacgccaa ggacgacgcg gggcacttca tcctggtgaa ccgcctgttc gcccgggtct
    19501 tcggggtgga gcccgaggag gtgacgggca agaccaacga ggattacggg gccactccgg
    19561 aggagaccgc ccggttcatg gaggaggacc gggaaatcct gcgttccggg aagcccctgt
    19621 tcgtgccgga ggagcgggcg ccccggcccg acgggagtcc cggctggttc cagaccacta
    19681 aaattccgta ccgccacccg gattgggaaa agcccgccat cctcggggtc tccgtggaca
    19741 tcacggagaa caaggaacag gcggaggccc tgcgccgttc ggcccggcac gaccccctga
    19801 cgggcctggc gaaccgggcc cttttctcgg agctattggg gaaggccctg gccgtcaccc
    19861 ggcgggagcg gaacaagctg gccctcctgt tcatcgacct ggacgacttc aagcccgtga
    19921 acgacaacta cggccacgcc gcgggggacg cggttctcga ggagaccgcc cggaggatcg
    19981 aacggaccct ccgggaatcc gacggcgcgg ggcgcttcgg aggggatgag ttcgtcgcct
    20041 tcgccttcgg gatctcccgg agggaagacg ctgcggcggt cgcggaaaag gtgcgggagg
    20101 cgatccgggc gcccatcgac ctcggtacgt cggtcctggg cgtcacggcc agcgtggggt
    20161 tcgcggtctt cccggaggac ggcacggacg aggaaagcct cgttaggcgt gcggacgagg
    20221 agatgtaccg ggaaaagcag gagcgttccg gaaagaagcg gccgcggacc tgaacccccg
    20281 gggttcgcgc cgacgaactc gaacaagaac tcgattccat ggatattcgc agtcccatga
    20341 attagactcg agcctgcggg ggtacggaaa tgttcgcacg aagggcgcga ctgttcctca
    20401 tgctaggcct ggccgcggcc ggcctggggg cccagaccct cagcctggtc acggacgacg
    20461 tctacccccg ctcgaccccg gatggccgcg gcttcgagga cctgatcctg aaggaggcct
    20521 tccgccgcgt gggcctcgcc gtccggatca cccccctgcc ctcggagcgg gccctcatca
    20581 acgtcaacca gggcatcgac gacggactct tcgtgaaggt cgccggggtc gaggcgggat
    20641 atgccaacct ggtaatggtc ccggaatcgg tctgcgacta cgagttcgcg gccttcggca
    20701 gggatcctac cctgaggatc acgggatatc cgagcctggc gccctacagc atcgccttca
    20761 tccggggatg gaagaatccg gaggcggcca ccgccggcat gaggtccgtg acccgggtca
    20821 aggacgacga ggccctcttc gagatgctcc tccacggccg ggtcgacgtc atcgtgtacg
    20881 aggccctgca gggacgggtc tggatccgga accacggcct gacgggcatc catcagctcg
    20941 gtcctcccct ggcgaccacg gagatgttcc tgtacctgaa cgtccgtcac cgggacctgg
    21001 cgcccaggct ggccgcggcc ctcaaggcca tgaaggcgga cggcacctgg cgcgggatcg
    21061 tcgattccgt ggtcggcggc ctctgacgcc atggccggag ggatcggtcc ccgggcgttc
    21121 cggcggatgc tcctggtctg cctccttctc gtggccggca cgaccgcggt gcagtacgtc
    21181 cggaggtacc ggagccggac cgaatccgcg atcgaggcgg cggagaacct ccggtccgtc
    21241 cagaccccgg cccttctgtc cgccctttgg agctcggaca cggaccaagt ccggatcctg
    21301 gtcgacggga tggccctgtt cccctacgtc aactacgtct cggtcgcgga ccaggacggc
    21361 cgcgtctact tcgccggggg catgaaaccc tcgagccgcc ccgggttctt ccccctggtc
    21421 tatccgtacg ggggccggga gatccccgtg ggcacccttg ccctggagat cgacacggaa
    21481 cggctcgcct cggacgtccg gtccgaggtc ctgcagtccc tgggcgccca ggccttcctg
    21541 ctggtcctgg cctccctgct ggtcttcctg ctcttcgagc ggatggtgac ccgccacctg
    21601 gtacggatcg tggagcacct ggacggattt tcccccggga aggtccgcgc ccccctgcgg
    21661 ctggacaaga agcccaaggg ggacgagctg gagatcctgg ccgactcgtt caacgccctg
    21721 gagcggggcg tggcggaggc ccgggcggac gaagtgcggg ccatggagga cctgcgggag
    21781 tccgaggagg ccgtccggcg ctccctccgg gagaaggaga ccctcctgca ggaggtctac
    21841 caccggacca agaacaacat gcaggtcatc gcctccctcc tggagatgca ggccctgatc
    21901 tccgaggacg agggaacccg gggggtgatg agggagatgg tggggcggat ccggtccatg
    21961 gccctggtgc acaagaagct ctacgagtcc aaggacctct cccgcatcga ccttggggag
    22021 tacgtcgagg acctgacggc gggaatccgg aacggattcc tggacgaccg gaagggcatc
    22081 gaaatccggg tggacgcgga ccaggggatc gtgtccctcc tggacgccgc catcccctgc
    22141 ggccttgccc tgaatgagct ggtcgtgaac gccgtgaagc acgccttccc gggcggacgg
    22201 gggggcctca tccgggtccg gctcgggcgg cgggacgacg ggatgatcga gatgcgggtg
    22261 gaggacgacg ggatcggcct gcccccgggc ttcgacgcgg agcgggacgg gcggctcggc
    22321 ctgcgcacgg tggtgtccct gatcgagtac cagcttcacg ggagcgtgga ataccggacc
    22381 ggggagggga cgacctggat cctggccttc cgggacggat tgttcgaagc ccgggtctag
    22441 caggttgtcg aaatactcga ggcatttctc caacctggta tgcaattgct cgagaacctt
    22501 acggttctct gcgcaattgc gacattaagg aagtgttgaa aaaggctcta tagccttttt
    22561 caacaccctg ctaggtcccg gcccgccgat cttcctcgat ccggtcgatc agggcgaaca
    22621 gctcttccga ggagatcggc ttggacagga aacccgccag gccggcctcc tcgcagagcc
    22681 tccggatctc gtcctcgtcg gacttgccgg ataccaggat cgaccggagc tttgggcgcg
    22741 tttcgtgcag ggaacggacg aactcgtccc cggagatccc gggcatcagc cagtcggaga
    22801 tcaggatgga gagccttgcc ccctcggact cgaggcgctc cacgaccgcc agcccctccg
    22861 cggcgtcgga agccctctcg taccgatacc tgtccccgta tctccgcttc aggagcatga
    22921 ccaggtgcag gaggatgatg ccctcgtcgt ccacgcaaag aatagcctcg ggggcggaag
    22981 tcggatccac ggcgtctcgc tcctcgggtg gtttccgatc gaagtatcga acggggcgat
    23041 cccggagtca aggaaccgcg gagctgtcca aaaagcaacg gacttatcgg accccccggg
    23101 ccgctgcgcg gcccgacacc cttgcagaaa tcccggtgcg cggctatcct gtccgtatac
    23161 cgacctggaa aggaggccga catgcggaca gcatacggcg ccccgatcca tgccgcgcgg
    23221 ccccgggcct gattcccggg ccgcttccag ataagcgggc cgtccggtac gccttcaggc
    23281 ttccgggcgg ccctcccctt gacctcgccg atccccgcgt gcaatacccc tgtcatgccc
    23341 atggtcgaac gccgctacgc cccgatatac gccttcctgg tatgcctctt cgcgctcttc
    23401 ggaacctcga tgaccgtcat cggggccacc ctgccgcgga tcctcgaaaa tttcggctgg
    23461 agctacggga ccgcaggagc ggtgatcgcg gcgaacgcga tcggctactt cgggtccgtg
    23521 ttcgcggccg gtaggatcgt cggccgcatc gggccccgga ccgcagccct gatagggcta
    23581 tccctggtgt ccggcggtct cgccctcttc gccgcctccc ccggccccgc cttgaacctg
    23641 gccctctacg ccctcatcgg gataggccag ggcttcctgg agatcctggt caactggtcg
    23701 gtgatgcgca tggaccggga aggccgggcg atgagcataa tgcacgcggc cttctcgatc
    23761 ggggccgtag ccggcccctt cgcggtcgga tggcttctgg cttccggacg gccctgggtc
    23821 ctgatctacc gcggcatggc cctcctgttc ggcgtcgttc tcgcggcggg cctcgccctg
    23881 cccttcgacc gtctgggccg ggaaaccgtc gcggcccgga ccggcggcgg cccctcggtc
    23941 cgggatcgcg cctatgtcct cggcttcgcg gtcctgttcc tgtacgtggg cgccgagctg
    24001 ggcatatcga actgggccgc ggagcttttc gtacgctcct tccgcggatc cgaggcgacc
    24061 ggctccttca tggtgtccct gttctggctg ggactcctgg cggggcgcct ggggattccc
    24121 ctcctcttgc cccgggcccg gcaggagaag gtcctggtcg tcctgtcggc gggcctcgcg
    24181 gcgagcaccc tcctgctcgc ggcctcgtcc tttttcggcc ccccggccgt ctttgtgggg
    24241 gccgcatccg tcgcgctggc gggcctgttc tgctcgtcga tctaccccat cgtgatattc
    24301 ctggaaggcc gggcccatcc tggggcggag ggcgcggcca taggattcgc gggagccggg
    24361 ggagggctgg gagccttcct cttccccttc ctgatgtccc gcatcgccgg cgccgcgggc
    24421 gtcaacgtcg gattcgcctt ctatgccctc gtggcggccc tggtctccgc cttggccctg
    24481 gcccttgccg cgaccacccg gggcggcgta ccggaccggg ggtgaagcgc ccccggacgg
    24541 agctgtccac cgggcggcat cctgggtata ttcgagaaag cggccgggag gccgcggaac
    24601 gattcgtccg acaagagagg tactttccat gtccgacagc atgacccgga cccggttcgg
    24661 ggacctcacc ctggagcgga atatcctgcg caactccttc gtctggatga ccctggggct
    24721 ggctttcacc ggcatcgtgg cctggggggt gtccaacagc ccgggcctcc tccgcaccct
    24781 ggtcctgaac cgtttcctgt tctacggact cgtattcggc cagctcgggg cggtctggtt
    24841 cctggcctcc cgggtcctca agatgagccc cgccgcggcg gccgcgacct tcgtcggcta
    24901 ctcggggctc accggggtga ccctctcggt gatcttcctg gtctacacct ccgggtccat
    24961 cgcctcggtc ttcttcatca ccgcggggat gttcgcctcc ctggccttcc tggggtacgt
    25021 gaccaagatc gacctgaccc ggatgggctc ctacctggcc atgggggtca tcggccttgt
    25081 catcgcatcg gtggtgaaca tcttcctgcg ctccgacggg ctcggctgga tcgtgtccct
    25141 ggcgggagtg gccatcttct cgggcctgac cgcctacgac gtccagaaga tcaaggccct
    25201 ctccgcccgc ttcggctccg aggtggacga gggcaactac ctgcgcctct cgatctacgg
    25261 ggccctccag ctctacctgg acttcatcaa cctcttcctc cacctgctcc ggctcacggg
    25321 ccgcaggagg taggtctatc cggggaggga cacctttagg aaaggtttcc ctccccggcc
    25381 ccttcccttc caaagaagcg gcccgccgcg ccgcctaccc gtcccgggcc gggggattcc
    25441 gccgcagatc ctcccgccgc tccctccagt ccggcaggac ccggtccagg atggcgtgga
    25501 accgggcgtt gtggttccgc tcgatcaggt gggccagctc gtgggcgatc acgtactcca
    25561 ggcactcggg gggccgggtc gcgagctcca ggttgagcca gacccgccgg gcccggatgt
    25621 tgcaggttcc ccaacgggtc ttcatccgct tgatgcccca ctccgcggcc cggactccca
    25681 gggccgtctc ccacttggcc cccaggggcg ggatcagctc cttgagccgt tcccggtacc
    25741 agccgtccag gatcgcccgc cgaccctccg ggcccgcgcc gggcgggacg aagagctcca
    25801 gcgtatccgg ccccgccagg cgagcgccga tcccccgtcc ccgctcccgg accgaaagcc
    25861 ggtacgcccg cccgagccag gataccgtgt ccccgctctc caggtccgag gagggccgct
    25921 cccgggcccg gacggctccc cggtggcgcc ggatccaggg cagcttctcg ctcaggaagg
    25981 cccggatcgc ggtctcgggc atgagcaggg gcgcggaggc caggacccgg ccgtccgggg
    26041 gccggatgac gacgcgcagg gtccggacgg gcctacgccg gagggtcacc tccaggtcct
    26101 cgacgagaat tgtggtttcc tggaacgtct tcaccgggcg cgacgctccg cgggatggtc
    26161 cgaggggcgg taccgggcca ggaaggtctc cacgagctcg cgcttgaggg cctccccgcc
    26221 gtcgggcacc gtaccgcccc cgtagaccgc cggccaggtg tacgctccgc cgagcagggc
    26281 ccggaacacc gcggccgcca tggcgggctc cggcgcgtcc agcttcccgg ctcgggcggc
    26341 ggcgcgcatc cagtccgcca ggggatcctc cgacgcggga tcccgggaaa ccgaggcccg
    26401 ggccagctcg ggggaacgca cgaaggccgc cacgagaagc cggatcatgc ccatccaccg
    26461 ctcgttccgg accacctcga gctcggcgtc cgcgaaacgc ccgagctggg tctcgatcgg
    26521 aacggccgga tcgtactcga agcgtttgaa ggcgcgcatc tcccggccga agcgggcggc
    26581 gatctcgtga agcaggatct ccttgctcgg aaagtagttg tacacggtcc gcttggacgc
    26641 tccggcctcc agggcgacca ggtccatgct cgaggcctcg aagcccccgc gagagaacac
    26701 ccggatcgcg gcctccagga tggattcccg cttctcctcg gagggcttcc gccccccctg
    26761 cgtaccgttc cggctccatc gctccatttc gccaccatac catgccgaca gcgggttcac
    26821 aattctggat tgacagtaag taaactatac ggtatagttt acttactgtc aatccaggac
    26881 agcaaggagt agaccatgga ctatgattcg aaggcggccc gccgcgcagg gctcttctac
    26941 ctggcggccg cggtgttcta catcttcgga atgatgtacg tggagggtcg ggtcgccggc
    27001 gacgatccgg aactgctccg caagatcgcg gaaacgggcg gactctaccg actcggcttt
    27061 ttgagcgccc tgctggggca cgtctgcctg ctggccacgg ccgcggccct gtacgagctg
    27121 ttctcccccg tcgacgcggc gtgggcccgg ctcctggtcg tcttcgtggt cgcgggggta
    27181 tccgtcgcct tcctgaaccg cgcccaccag ctggtcgtgg agcgtctggc cgtctccgcg
    27241 taccccgggc tcccgggaac cggagcggaa tggctggccc cgcttttcat cggcctgtac
    27301 cgggacggcg aacgcctggc ctgcatgttc tggggactct ggctcctccc cgccggggtg
    27361 ctcgccttca agtcgggatt cgccccgaag gccctgggcg tcgtgctgat cgccgcctgc
    27421 gccttctacg tgctccggtt cttcgagtac ttcctcctgg atccgccccc cgcggccctg
    27481 cgggcccccc tggccgtggc gaacgcggcg gactccgcgg cgaaggtggc cgcggaactc
    27541 ggcttcatcg gctggctcct ggccgcgggg ttccggcgcc gcctcccggc ggaccgccgg
    27601 gaatccgcgt cagccgcgcg cccctaggct ctccgaccgg gttcggaaga agacggccgc
    27661 caggatcgcg gccgccgcgg gatacgcgac cgagagggcc gcgaagaggc cgatcccgcc
    27721 ggaccagagg gacagggaca cgttgaagca gtagtgggtc cagaaggcga ccaggaggtt
    27781 gtcgctcctt ttcatcagga cacccaggat caccgtcagg gaggtgagga cgaccacgtt
    27841 cgcccccgcg aatacgaggc cctccaagcc ccggaactcc gaggcgagca cccagagggg
    27901 ggcgtgccag aaggcccaga ccaggcccag gaggaggttc cccttcagga atccgtaccg
    27961 gctctcgagc tccggccgca ggtaaccccg ccaggcggac tcctcgccgg agggtcccag
    28021 gaagagcatg agcgccgccg taccgagcag ggccccgggc tcgatcgaga tccccgcacc
    28081 gggtctcgag ccgccgagaa cggccgcggc cgcggcggcc gccgcaaaga ggccgaacac
    28141 gagggccggg atccccagga ccatgccggg ccggatcctt cccgcgaaaa ccttggcgta
    28201 gaaggcgcgg accgagccgc cgggcaggaa cctgcgccgc agggccagca gtaccagggt
    28261 cggggaccag gaaccgagca cgacgatccc cctcatcacc ggctccgggg cgcccaagac
    28321 catcgaggcg aatcctccga ggcccaggat gaccgcccag aaggtcaggt aggcgatcgc
    28381 gatgaatctc cacatgctca gctcctttcg cgacaaccca ctagataccg agctcccgca
    28441 tcgtccggga tacgcgccct gtccgctctt cgagtccctg cccgtccgga ggcccgagct
    28501 ccagctcccg gtcgatccgg ccgtcccgga ggaagagcac ccgccccgcc cgggccgcca
    28561 ccgcaacggc cccgatgaaa atggacggct tttgcgggtc ggcggagctt tcctgcgcga
    28621 cgggaagcct ggcccccgcg gcgtcggaca ggcaacgctg cagaaaaaca gcggccgcct
    28681 caagatgtct cgccgccgga gagcccacct tgtcgggaac cacaatctga tagtccgact
    28741 tgccgtctcc ggccaccaca agctccccgc caaaggcggc cgcccgcggc aaaagtgcgg
//
LOCUS       DAOTEI010000024        28642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1080_length_28642_cov_5.652359, whole genome shotgun sequence.
ACCESSION   DAOTEI010000024 DAOTEI010000000
VERSION     DAOTEI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 28642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28642
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1080_length_28642_cov_5.652359"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="P5313_03875"
     CDS             complement(<1..217)
                     /locus_tag="P5313_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613792.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRY79537.1"
                     /translation="MESTYSAQNIQVLKGLEAVRKRPGMYIGSTGLDGLHHLVYEVVD
                     NSIDEALAGVCDRIEVVLEPNDIVRVTD"
     gene            685..2061
                     /gene="dnaA"
                     /locus_tag="P5313_03880"
     CDS             685..2061
                     /gene="dnaA"
                     /locus_tag="P5313_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRY79538.1"
                     /translation="MNEIDYSPFWNESLKQLEQELGEHEFAIWFNIEYAGATPDSIRV
                     RVPSAFYRDQFVKAYQKTFEAKLADLTGKNLTLEFLVEKKQAAPEPAKGGAPKSPDPA
                     APAAGRPARSKHPSLRTDLTFENFVIGENNSFAANAALAIAKNPGTAYNPFLIYGGVG
                     LGKTHLMQSIGNRVWAEAGKAKIICLSAEEFTNEFIQSIHDKTTHAFKNKYRQADILL
                     IDDINFLEKKMGSQEELFHTFNALYDTNKQMVFTCDRPVSELKNLSDRLRSRFERGLN
                     VDLQPPNYETRCAILRKKAESAKLPVPTEVTDIIAKNISTNVRDLEAALMKLSAYAAL
                     VGKSITLEIAQQQLKDVFSQSKHGNVTIDHILRVVADHFKISYGDLKGKKRTKNIAYP
                     RQVAMYIAREITEYSTTELGLEFGGRDHTTVMHGCQKIEERLKLDPALETNIQQIVAA
                     VKEMSTKG"
     gene            2512..3615
                     /gene="dnaN"
                     /locus_tag="P5313_03885"
     CDS             2512..3615
                     /gene="dnaN"
                     /locus_tag="P5313_03885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRY79539.1"
                     /translation="MKFTCEKNAFAKEISFAQEIIASKNALSIMSNVLLEAADGALSI
                     RATDIKVGFETRIPVDVAVPGSVTVFCDKLSGILSSIPEGDIEVEYDDAKVVVKPQFK
                     KVRFQLKTISSEKFPELPQADDAAWFALPAKDLKEMITQTVFAVSDDETRYFMNGVFL
                     ERTPEGLVMVATDGRRLAYIRKDIDPSVPDFKGVIVPPKILSLILKRAADEGPVDLAV
                     TEKNLFVRFGTYRVSSVLIEGQFPNYQKVIPQSQSRFFVLNRAETLDALRRVSIFVEQ
                     KSRRTFFNLSAGSLAVSSEESELGTAKEEIPCRYAGEDALLALNYKYLEDPFRVIKSD
                     EVKIEFTEPNRAITVRPEPETDYFHIIMPMQLE"
     gene            3625..4716
                     /gene="recF"
                     /locus_tag="P5313_03890"
     CDS             3625..4716
                     /gene="recF"
                     /locus_tag="P5313_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709718.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRY79540.1"
                     /translation="MFETVTTLRFRNLADASTGVGARRVFLVGDNGQGKTNFLDAVYS
                     LCYGASFRGATDAEAARHGQEEWSLAADTRAGSEDPPSRVTVSFRAGRKAAALDGKAV
                     PDRKAMIERNPAVVFCPSDMDFAQGEPERRRFFLDQTASLVSPGYLDSLRAYRKVLRM
                     RNQSLKDGRLDVLEVLDEQLAEYGTVLRDRRAEILGLFDPVFAERYEAVARLGIRVGL
                     EYRPSWKPGTGPEEVVAALRARRRDELALGVTLSGPHRDRFRFTREGGDFAAFASTGQ
                     LRLLALTLRGAQAGFCAERTGRKPLLLLDDVLLELDPEKRRRFMEGLPPHGQAFFTFL
                     PGEPYGDYADDSTMVYWVDDGRLSDKDRR"
     gene            4685..5218
                     /locus_tag="P5313_03895"
     CDS             4685..5218
                     /locus_tag="P5313_03895"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRY79541.1"
                     /translation="MDDSRIRTAAELISALISPKAAEAAGTWAKVTGAWPALAGDRAA
                     PHSRIADLENHVVTVEVDHPGWIQLLQFRQARLLTEIQERFPSLEVRALQFRLARRGG
                     PGPAGAADGAGRGGSSGDTGRPADRNDAGPGTVAGHPEGPESPPPAAPLRLDPEAVGD
                     EALRKALSGLRDAYEGR"
     gene            5348..5503
                     /gene="rpmH"
                     /locus_tag="P5313_03900"
     CDS             5348..5503
                     /gene="rpmH"
                     /locus_tag="P5313_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRY79542.1"
                     /translation="MKRTYQPSRTRRVRKFGFRARMKTVGGRLVLKRRRAKGRIKLTI
                     SDEGKKY"
     gene            5508..5906
                     /gene="rnpA"
                     /locus_tag="P5313_03905"
     CDS             5508..5906
                     /gene="rnpA"
                     /locus_tag="P5313_03905"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRY79543.1"
                     /translation="MGNDAGRARRCTFRKRERLRERSDIDRAFKQGTRYSVRGMRLHV
                     VPNGTAAVRALFVPVRKYGNAVLRNRARRLASEAYRLVKSGVAPGRDLVFVLYPGTDS
                     FETRSGQVERVLRLAGAMPGGSCELSSRRS"
     gene            5879..6100
                     /gene="yidD"
                     /locus_tag="P5313_03910"
     CDS             5879..6100
                     /gene="yidD"
                     /locus_tag="P5313_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009795302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRY79544.1"
                     /translation="MRALLSAVLIGLIRFYRAVISPLKPPSCRFYPTCSEYALEAVRK
                     HGPWKGTGLAVRRILRCHPMNPGGFDPVP"
     gene            6100..7935
                     /gene="yidC"
                     /locus_tag="P5313_03915"
     CDS             6100..7935
                     /gene="yidC"
                     /locus_tag="P5313_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRY79545.1"
                     /translation="MNETRSDKAFDSKTILAIVLSVLVMAGGTWIQQKFWPTQPAPAA
                     PPAATQPAAAQPATAPAAVSPGLPTPGAAAPVLAAAEFEAPSAEATYTVKTDVMEAVF
                     TNRGGELVSLRLPEHADKDGVVELIDAPRGEAQAFALALGGPNAPPLRELMNFRRIDD
                     RTVEFWRTFLPAAGGTDPFTLRKRFTFVPGEYMFRLEIVLENSVNDVLPLTTDGFAYT
                     LGYGPRIGPTIHEGGRYADFRKFAALENGKKKNINPKGGVETTDRRAAWSAVAGKYFT
                     LIVLPDATPYTTTYRNHQENGKTDLSSLAFSRPVVKASRQTDIFHIYFGPKSSRELVR
                     YDDSSKNAFGRTGDKLEEVMEGGNVLGWLEAILKVGLNFFHGLIPNYGIAIILLTILT
                     KVLMYPITAKGSQGTARMQELQPKIQEIQAKYKNNPQKMNQEMAEFYKREGYNPMSGC
                     LPLLIQFPIFIAMYNLFNNHFDLRGASFIAGWISDLSQPEAIFKFAEPINLVIWKMSA
                     IRGLPIIYVASQLLYGKFTQQPQSGGQSASQMKIMMYGMPIMFFFILYDVPSGLLVYW
                     IVSNVLTIGQQAVINRMIHQKKLAAAAAAPAPVIAPVKGRKGKGR"
     gene            7940..8740
                     /gene="jag"
                     /locus_tag="P5313_03920"
     CDS             7940..8740
                     /gene="jag"
                     /locus_tag="P5313_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRY79546.1"
                     /translation="MVYEFEGRTEKEAIDVAAAELGLARDEFDVEILETQSGGLFKKG
                     KVRLRIHVHSNARPAPISEAAAAQAAGRPIPEPRYDREGRGGRRDRGEERAEEPRTAA
                     PRAAATDPAGADEFEAKVLEFVKTTVEKMGYACEVSVLFREDKKLGVRLDSEHASILI
                     GKKGKNLDALQLLVNVYGGRLGREDLRIVIDSENYRIRREEALVKLAYESAEKVRRSR
                     GSLLLEPMNPFERRIIHTTLNDIVDVETKSEGEGLYKQVRVLWKGAKR"
     gene            8850..10493
                     /locus_tag="P5313_03925"
     CDS             8850..10493
                     /locus_tag="P5313_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY79547.1"
                     /translation="MGIQSAIRKRKSPTVPVPGEAGSRGSGRTAFDELSRASALLSSG
                     TDFGELESILVEQACDITRSDLAALYLYTGDPRDPKTPLRLSYKRGPWSVPASLARSG
                     EALSFLEDCGEAAVLNRRRPCALEGILLHPEMASGMALPVAVPKAAVGILFVNARTEN
                     HYDRIRLNFLGSFCALAAGMLRSSRLFQELKEHVAVIESLERYQESVFSSMTNLLVTT
                     ERDGSVRYFNEEAARAFGLSEEDVGRPLNDLLGTRVDAKILRTVEETLSKGSTILGVE
                     GIAAGARGDLDFSLNLSPIRGKRSDRHDGLTLLFTDQSRERALKERMNVVVEERRAIK
                     DMFSRYLSSEVVQHLMESPDQVRLGGAGKKATVFFADIRGYTSFSEGRTPEYIVEVLN
                     DYFSQAVEVVLQYKGFIDKFIGDCIMAAWGVPMTSEAEDAVNAVTCALEIQKLIEDPK
                     RTFFRGDAEHLRVGIGMHTGYLVAGNLGSSRRMNYSVIGDTVNVAARLEGVAKAGEVI
                     ITEDTRGLLGSAFQLEEREPVKVKGKVEPIHIYRAVKRR"
     gene            10510..13107
                     /locus_tag="P5313_03930"
     CDS             10510..13107
                     /locus_tag="P5313_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY79548.1"
                     /translation="MERIVGFLAGISTGTWIAAGIAAAVAAAAVLILSAQARFRRFVR
                     TAALDPAALGGRRLSPGTLALRERYVIRRARGGFRGNPGPFPRLPATLGYGERWMSAL
                     ESGGGRGTMSRVLEFLPDTGLFACFRAALRREALGRQLLEWTGTDAGAFALRRIALSG
                     PGRDFDGPKARALLAERLDEVRELLGDPEWAVRVFAVRILLGDGEERTARSLRSCLAD
                     PHPLVRRLIVESWTDPDRKGFYEGLYGLLVSDSVLEVRKAARARIRKDFADLYSPDPG
                     SLGPEQVLHFLELLDPADPSDEGIAFKYLEADSPEERLAAAECLQAGGALARVLETAE
                     LGDAVELERRFRLLDAAAELQVTGFLSKVETIEGDGAFHLAARILARAGDRKHIATLA
                     KRWFSRMGMPPYTPERLGIYDLVLAASRARGGEDACELVVGELNARRGQPELASNILM
                     SLEGNQGPCVFPALQGLFLDPTFELRAQLRAALLGQPREQVVPLALSLVRADRVSLPR
                     VVRKDALYLIGELKLTGALQRALESLPLLDADEISDFAPVLAGADMKAFERKARVILD
                     GADAPTRAAVIAALPAAGSKAFLADVKAALRDADPDIRVAAARALAGFHEGKALASGG
                     LDLLRDPVERVRFAASAALAMEGGAAVLQGLRSVLDDPNEVDDVKRSLIEGLGRADDV
                     GSLDLLADILGEKEGHREEIFKALAARVSKRDLERLVERFKDATGDLKTLLSEAFRRM
                     GETGERTMAALLEEDIASLKPYIVQVLESRGYVEARILELKHRDPKVRRDAAAALSNV
                     GSLQAFRGIVLAARDPDPEVRVAVTRALERLAGPEGASLLAELEADPNPRVRKYVLWA
                     LERVKAKNL"
     gene            13131..14099
                     /locus_tag="P5313_03935"
     CDS             13131..14099
                     /locus_tag="P5313_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRY79549.1"
                     /translation="MKNRKSALILVLILLAAPAWAQLQKPTAEVRRFQVEALTLRDVT
                     FLFELAVKNPYPVELSFSGMTLDFSVEGAKVFSAQSKGGFKVPARQEKANTFTVTLEY
                     EAVIRMVKEYASKEWLNTVIDGVLVIPLPKLPGLPQDVKFTYKLEKKIPAIKPQVALV
                     GFSVEPPTAAQVTQALAKAGKKADPAKAQKVFADVLAGRKPEAPVIDPAELDLPLSVS
                     FTLEIRNDAKGPLSFQKLGYELFVNGESLVLGDSSGVVREGARTLVKVTNTFSSKKLS
                     KGVRAIFSDRKGSFAVKGSASIKLPDEIRKEPVPLAFHETGTFSLK"
     gene            complement(14191..16128)
                     /locus_tag="P5313_03940"
     CDS             complement(14191..16128)
                     /locus_tag="P5313_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY79550.1"
                     /translation="MKRTVLSMLEEAERAYPNVNYVLKKTDAGWEGWTFGRVRSEARA
                     FASFLLGRDMKRQDTACILAEGSPEWVAAEFGVLMAGGISVPLSIKLLSEEIPFRVKH
                     SGSKVFITTGNQLEKVLTALVSTDAKKTLVVYLDEDEERPDAVCGKTGFPRKNIVRFK
                     DALQEGRTALPARTPALKDIEDATAEDDTVTICYTSGTTGDPKGIMLTHLNYWANCKD
                     GIELFEKPYFFTTLIILPVDHSFAHTVGIYAALLCGISLYFVDPRGGGIAILRNIPIN
                     LKEVEPMFLLTVPALSGNFMKKIVAGIEEKGGFIEKLFKAGIAAGIAVNGNGFNPVPF
                     GVRARNALVHGIAKALIFNKIKKMVFGKNIRFCVGGGALLDVKQQEFFAAFGVPIFQG
                     YGLTEAAPVISSNTPSKHKFGTSGIVAPTVRCRILREDGTECAVGETGEIVIRGENVM
                     KGYFRNPEASAKALRDGALYTGDLAYYDKDGFLVVVGREKALLIAADGEKYSPEEIEE
                     AIGTSTDCFAQIMAYNEQSKYTAGIVSLETGPVERMIKAEGIKTAAALMDRLAEEFYR
                     FKSDPKAKKVPANWIPATFLIVPEGFSDKDGTVNSTMKLVRHRVAKVHAEGIAYLYGS
                     EGSRPANPRNVETLRKMFNLA"
     gene            16250..17524
                     /gene="prs"
                     /locus_tag="P5313_03945"
     CDS             16250..17524
                     /gene="prs"
                     /locus_tag="P5313_03945"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRY79551.1"
                     /translation="MNYSDPTRLGILACPGGERFTELVLQRLASIYKRRFERKTRVLA
                     DRYHLSAELVIRKSNFANDIQSSMLYIPGDVNKFRAPRFKINARHSWFANGEIKTEIL
                     DSVRGKDLYIVQDVENHQPVKFNEGRDERVLSVNDHIFNLLVAVDAAMQSGAERITVV
                     VPTYPYSRQHKKKGREGLTAARIGQILENMGVSRIITLDIHSREIENCFNKLRLENLH
                     ASYQVIQKLATVVDVNCDDLVVLSPDTGAIDRNKFYASTLQKPLALIYKERDYSRVTK
                     NAEESNITDMKLLGDIRDKIVFMADDMLGTGGTLLKAMQFLRDQGARQVICAVSLPMF
                     SATAIQDFDAAYREGRFHRIIGTNAIHHDELLKKEWYVQADVSTLFAQIISLVYHNHS
                     LSPMLDNRGLISKMLKKSQQAACPPQGNLFQE"
     gene            17521..18228
                     /locus_tag="P5313_03950"
     CDS             17521..18228
                     /locus_tag="P5313_03950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY79552.1"
                     /translation="MRVFRLPAAPRALLFDLDSTLYTHAEYAAFQNEVLVGELARRRG
                     VDEERIRAEIRAVRDRVARETGKVTSLGNAMAALGVDIPTSVAWRERLIKPGDWLTRD
                     DRLAEALSALSARFVLAVVTNNPRSVGRETLRVLGVEDSFREVVGLDDTMISKPAREP
                     FELAARRLGVPAGDCVSVGDRYDVDLAVPLELGMGAVLVDGAEDVYRLPELFKTAGKP
                     KDGRFPDLTTEITESTE"
     gene            complement(18424..19215)
                     /locus_tag="P5313_03955"
     CDS             complement(18424..19215)
                     /locus_tag="P5313_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY79553.1"
                     /translation="MEIKGSVAVVTGGASGIGEAVAKTLAAKGASVVIGDMNQAGIDR
                     VVAEIKKAGGKAAGKTLNVTDDADTAAFMDFAVKEFGAINVVVPCAGIIKDGLMISPD
                     KETGKVKRVLSTADFRAVIDVNLVGPFITIREAASRMVDNGWKGVLITISSIQKQGGV
                     GQINYSSTKAAMALWPKILVGEFQMKGITGIRVASIAPGYVGTPMVKGMDQGALAKIL
                     SGVHLGRLIEPEEIAETIAFIVSNDAVNATCIEVAGGMISGLIAK"
     gene            19434..20990
                     /locus_tag="P5313_03960"
     CDS             19434..20990
                     /locus_tag="P5313_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HRY79554.1"
                     /translation="MKSDKIMSAADAVARIKSGAVVATEGFVGNGFPEELAVALEQRF
                     KETGAPRDLTLIYAAGQGDGKTRGLNHFAHEGLVKRVIGGHWGLAPALGKMAMENKIE
                     AYNLPQGCVSHLFRAIAGKRPGHITHVGLKTFVDPRNGGGKINAKTTENIVELLTLDG
                     KEYLRYRTFPIDAALIRGTSADSKGNISLEKEALYLENLAIAQAVHNSGGVVIAQVER
                     VVEDGFLNPKDVVVPGILVDCVVVAKPENHWQTFATQYNAAYASEVRVPVASLKPMEM
                     SERKIISRRAAMELHPGTIVNLGIGMPEGVAMVAAEEGILHDFTLTVEPGPIGGVPAG
                     GLDFGAATNAECLVDQPSQFDFYDGGGLDLAFLGLAQVDEQGNLNVSKFGPKFAGAGG
                     FINITQNAKKVFFLGTLTAGGLEVAVEGGKLKIVKEGEVKKFVKAVEHKTFSGEYAAQ
                     VGQTVMYITERAVFTLTKEGLELTEIAPGVDLKKDVLDLLEFKPVVRGTPKLMDSRIF
                     ADKPMGLSKN"
     gene            21111..21761
                     /locus_tag="P5313_03965"
     CDS             21111..21761
                     /locus_tag="P5313_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79555.1"
                     /translation="MTKRTNKRQEQAASTKAALFESAITLFNEKGYYSVTIEEIAQRA
                     GTAKGSFYTYFRTKSDIIIEEFRAIDDYYKRFEKTLPRFPNALEKIYAFNRAQMKYVR
                     DQVGLEMLKILYANNILEPSTEKILINTNRYLHGLIRGLIEEGQREGLFRTDVSADRL
                     ALLYTRGHRAVFLDWAISNNAFDLVKDGVEFCKIVMCPALMAKSVPATGTDSEPDK"
     gene            21964..23223
                     /locus_tag="P5313_03970"
     CDS             21964..23223
                     /locus_tag="P5313_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanoyl-CoA acyltransferase"
                     /protein_id="HRY79556.1"
                     /translation="MSKVSIVGAYNTKFGSFVNKNKETGEITDTCSLYELMAEAAQGA
                     VKDAGLDPAQIDGVWIGSFAPGLFTSQDHLGPLALESFPDAFRFKPTQRVEGACASSS
                     MAVYNALYAIESGRFRNVLVVGVEKMNLLKTAGVTHALAGASYWPTEGGVGMTFPGLF
                     AEYAKGYAKQYGLSPEKLRSILAAVAALCYKNGLENPLAHFGKGGPSDKLGLHTAANI
                     LGLPDEKNPMVAEPLRLHDCSLVTDGAAALVLSPTEEAKRRAGPAVEIAGIGHTTERM
                     AIKDRVNMYDLIAAKKAVQAAYKEAGISAKDLRAAEVHDCFTITQLLCTEALGLSGDG
                     KSGFEYEAGRFGPGDDCPVNLSGGLKAKGHPVGATGASMHVLLYKQMTGKPIGQAREK
                     GAPEVTATLNVGGSAVTNAVSVLRRIR"
     gene            23409..24788
                     /locus_tag="P5313_03975"
     CDS             23409..24788
                     /locus_tag="P5313_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRY79557.1"
                     /translation="MLGVIGIIVSLGLLMYLAYRGISVLILAPILALFAAVFSGMPLI
                     ATYTQVFMPNLANYLKLYFPVFMLGAIFGKVMEETGAAKAIAHAIATKLGKDKAILAV
                     VLTCAVLTYGGVSLFVVVFAVYPIAAHLFREAGTPKRLIPGAIALGSFTFTMTAFPGT
                     PQIQNAIPMPFFKTTVYAAPVLGIIAGIIMFVGGTWWLMARAKTLNAKGEGYGNHPNE
                     QIKEFDSSNLPSVLVALIPILIVLVLNYIIVTFIYTANNPAYEYLKAKPFETNINSVR
                     GNWGLIIALIAGIVVAILMNLKRLKVKETVNAGVFGSMLPILNTASEVGYGNVVKTLS
                     AFAIVQAALLSISDNPLITSAIATNVLAGITGSASGGMSIALGAMGETFYNQAIAKGI
                     NPEVLHRVVAIACGGLDSLPHNGAVVTLLGITQITHKEGYADVGMVSVVIPVLTCVVA
                     IILGSIGIV"
     gene            24865..25713
                     /locus_tag="P5313_03980"
     CDS             24865..25713
                     /locus_tag="P5313_03980"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRY79558.1"
                     /translation="MIGTIGVVGAGQMGGGIAQVCAQAGYEVVLFDVVEAQLAKARSG
                     MEKSLRKLAEKGGIDPAAVEPALARVKTVTELEAMAAADLVVEAVNENEALKLEIFRK
                     LDGILRPEAILASNTSSISITKLASATKRPSKVIGMHFFNPVPLMKLVEITRGHETGD
                     ETYAAVAELAAKVGKESAVVQDYPGFIVNRILVPMLNEAAYAVYENLATPEDIDKAMK
                     LGTNQPMGPLTLADFIGLDTCLAIMEVLYDGFKDPKYRPCPLLVKMVRAGYLGRKSGR
                     GFFVYG"
     gene            complement(25710..26537)
                     /locus_tag="P5313_03985"
     CDS             complement(25710..26537)
                     /locus_tag="P5313_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79559.1"
                     /translation="MADRKKLASLKKKLAPRKPKPTKAPGKPDPKFLDRLSKALRDEI
                     EEAGPSAANAAAARARPAPEPAPEQDAPARGRGRASAVEEPVSLSSGSPPRRAGASAA
                     ARASGKKRKAALLKELKSVLEELDEEGLDFLLEQAHVHRYNMEVERLNEAEERREAGR
                     AEAVSIRAGKRPAAGGRTGPRSPALRIERSQDGQTYHVVSAGRYKMFSADEMLALVRI
                     AHANPDAAEAGRGLHRWMSRERTDALADLGFAGPSDPGLAALAALLRKSFAAPPKRA"
     gene            26646..27302
                     /locus_tag="P5313_03990"
     CDS             26646..27302
                     /locus_tag="P5313_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRY79560.1"
                     /translation="MNHEEFYESCREAREELAYFMRRLYERGLTTCSGGNLSARVGEA
                     HVIVTPSALDKGRITGDQVGLITMDGENRTPHLKASIETGMHLAAYRARPDIRAIVHA
                     HPVTATSFAARPGGIDTTLTAEAYTVLGRPAVAPYALMGTRDLADRVAERLAVADVVI
                     LENHGILTVGRTLLSAFDRLEVLEAAAKMTLYTALLGGAAPLTPERLTELDAWKAARK
                     "
     gene            complement(27376..>28642)
                     /locus_tag="P5313_03995"
     CDS             complement(27376..>28642)
                     /locus_tag="P5313_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRY79561.1"
                     /translation="VMGSKRLKAVVAAPAHKNPRPVHDSAALAEACTAFAREMPGKTE
                     RLVKYGTAGIVVSSQDLGDIPVRNWNEGSFGDRAAPLSGQHLAESGRLKKRFFCGRCP
                     IGCGRTVELSDGTLGAGPEYETACMYGSNCLVGDPEAVIAANELSNRLGIDTISAGAA
                     IGFLMESFEKGETAGIDLGSVVPAWGDGKALVELVRMIGERRGAGELLGEGVLRASRV
                     IGRPEYAIHSKGLEFPAHDPRAFNSLALSYATSNRGACHLQGFTYVFEKAITLPERGF
                     PAAQDRFGTERKAELVIASQDLMGLFDSLKLCKLAMNGGVGATVALGWLNLATGWDMG
                     MEEFLAAGERIFNLKRAYNCALGVSRKDDTLPDRIRFVPKGGGAGDNLPPDLDASLDE
                     YYRLRGWSPEGIPKPETLKRLGIEYPGKS"
BASE COUNT         5083 a         9468 c         9308 g         4783 t
ORIGIN      
        1 tgtccgtcac ccgcacgatg tcgttgggct ccaggaccac ctcgatgcgg tcgcagaccc
       61 cggcgagcgc ctcgtcgatg gagttgtcca ccacctcgta gaccaggtgg tgcaggccgt
      121 ccaggccggt ggaaccgata tacatccccg ggcgtttgcg gactgcctcg agacccttca
      181 atacctggat attctgggca gaataggttg attccatgcg ggtacctatc atcgtggtat
      241 atcgcggcgg cgttcccgcg ttccgggacg gacgagcgcg gatctgcctc catcgttcga
      301 tggaagaaag gacttgccga acctggtttc aggattatac tcgttcccgg gaggaaagac
      361 aaggcgaccc gcctttcgag gacaggaatt acaggcttcc gagtcgttgt ggatatcctg
      421 ttgatagttt tataacgcat tgctcatcaa ggaataacaa gatttcggac ccgttccgcc
      481 ccgcggccgg catccttgca gtgaaattat tttattgtct cataattact tacaagagcc
      541 ttccgaggtt cgagagtcct tgattgtgga taaaccggag agtatccctt ccgggattct
      601 tgacaggccc gtgggtcttc ttgattgatt gtggataact tgtgaaaata cggtaccgta
      661 cagggacagg aaaggcgtag caggatgaac gagatcgatt attccccctt ctggaacgaa
      721 tcgctgaaac agctggagca ggaactcgga gagcacgagt tcgccatctg gttcaacatc
      781 gagtacgcgg gcgcgacccc ggacagcatc cgggtccggg ttccttccgc cttctaccgg
      841 gaccagttcg tgaaggccta ccaaaagacc ttcgaggcca agctggcgga cctgacggga
      901 aaaaacctga ccctggagtt cctggtcgag aagaagcagg cggctccgga gccggccaag
      961 ggaggcgcgc cgaaatcccc ggatcccgcg gccccggccg ccggccggcc ggcccggtcc
     1021 aagcacccct ccctccggac ggacctgacc ttcgagaact tcgtgatcgg ggagaacaac
     1081 tccttcgcgg ccaacgccgc cctggccatt gccaagaacc cgggcaccgc gtacaacccc
     1141 ttcctgatct acggcggcgt gggcctgggc aagacccacc tgatgcaatc catcggcaac
     1201 cgggtctggg cggaagccgg caaggccaag ataatctgcc tgtccgcgga ggaattcacg
     1261 aacgagttca tccagtccat ccacgacaag accacccacg ccttcaagaa caagtaccgc
     1321 caggcggaca tcctcctgat cgacgacatc aacttcctcg agaaaaagat gggcagccag
     1381 gaggagctct tccacacctt caacgccctg tatgacacga acaagcagat ggtcttcacc
     1441 tgcgaccgtc ccgtgtcgga gctcaagaac ctgtcggacc gcctgcgcag ccgcttcgag
     1501 cggggcctga acgtggacct ccagcccccc aactacgaga cccgctgcgc catcctccgg
     1561 aagaaggcgg agagcgccaa gctgccggtt cccacggagg tcacggacat catcgccaag
     1621 aacatctcca ccaacgtccg ggacctggag gcggccctga tgaagctgag cgcgtacgcc
     1681 gccctggtcg ggaagagcat caccctggag atcgcccagc agcagctcaa ggacgtcttc
     1741 agccagtcca agcacggcaa cgtcaccatc gaccacatcc tgcgggtggt ggcggaccac
     1801 ttcaagatct cctacgggga cctcaaggga aagaagcgga ccaagaacat cgcctacccg
     1861 cggcaggtgg ccatgtacat cgcccgggag atcaccgagt actccaccac ggagctgggc
     1921 ctggagttcg gaggaaggga ccacacgacg gtgatgcacg ggtgccagaa gatcgaggaa
     1981 cgcctgaagc tggaccccgc cctggagacg aacatccagc agatcgtggc cgccgtgaag
     2041 gagatgagta ccaagggata attttcttgg actcaccacg gaggacacgg aggacacgga
     2101 ggatacggag taatttcggg gaaaagcacc cgaaacgtcc gtcgagccgt gcatggatga
     2161 ggagcctcgt ggatatgtgg atatcgatcc gaaaatttcg ggaaaatcat ccccagggca
     2221 agtaccggat cctgcgatac ttgcgccgac tatccacaga aacctttccc ttattactac
     2281 tgctactgaa tttaataaat tctacaaaga caataactgc gccccgattc ctgacattct
     2341 cccttcgccc gtttcagatc tacacctacc acggggcatc agcctttagc cgcggcgtcc
     2401 ttatctaatc tcttctccgt gagctccgtg gtgagttcct gagacacgtc tctcccgttt
     2461 tccattgtcc taggccggaa aaacatggta cagtaccccg gaggtggaaa catgaagttt
     2521 acctgtgaaa agaacgcctt cgccaaggaa atttccttcg cccaggagat catcgcctcc
     2581 aagaacgccc tgtccatcat gtccaacgtc ctgctggaag cggcggacgg ggctttgtcc
     2641 atacgcgcca cggacatcaa ggtcggcttc gagacccgca tccccgtgga cgtcgcggtt
     2701 ccgggatccg tgacggtctt ctgcgacaag ctgtccggca tcctctccag catccccgag
     2761 ggggacatcg aggtcgagta cgacgacgcc aaggtcgtgg tgaagcccca gttcaagaag
     2821 gtgcggttcc agctcaagac gatctccagc gaaaagttcc cggagcttcc ccaggcggac
     2881 gacgcggcct ggttcgccct ccccgccaag gacctgaagg agatgatcac ccagacggtc
     2941 ttcgcggtgt ccgacgacga gacccggtac ttcatgaacg gcgtcttcct ggagcggact
     3001 ccggagggtc tggtcatggt ggccacggac gggcgccggc ttgcctacat ccggaaggac
     3061 atcgatccct cggttccgga cttcaagggc gtgatcgtcc cgcccaagat cctctccctg
     3121 atcctcaagc gcgccgcgga cgaggggccc gtggacctgg cggtcacgga gaagaacctc
     3181 ttcgtccgct tcggaaccta ccgggtgagc tccgtcctga tcgagggtca gttcccgaat
     3241 taccagaagg tcatccccca gagccagtcc cggttcttcg tcctgaaccg ggcggagacc
     3301 ctggacgccc tgcgccgggt ttccatcttc gtggaacaga agtcccggcg gaccttcttc
     3361 aacctctccg cggggagtct cgcggtctcc tccgaggaga gcgagctggg caccgccaag
     3421 gaggagatcc cctgccgcta cgccggagag gacgccctgc tggccctgaa ctacaagtac
     3481 ctggaggatc ccttccgggt catcaagtcc gacgaggtga agatcgagtt caccgagccg
     3541 aaccgggcca tcacggtgcg gcccgagccc gagacggact atttccacat catcatgccc
     3601 atgcagctcg agtgatccgg gcgcatgttc gagaccgtca ccaccctccg cttccgtaac
     3661 ctggccgacg cctccacggg cgtcggcgcc cgccgggtct tcctggtggg cgacaacggc
     3721 cagggcaaga ccaacttcct ggacgcggtc tacagcctct gttacggggc ttccttccgg
     3781 ggggcgacgg acgcggaggc ggcccggcac ggccaggagg agtggtccct ggccgcggac
     3841 acccgggccg gctccgagga ccccccctcc cgggtcacgg tgagcttccg ggccggtcga
     3901 aaggccgcgg ccctggacgg gaaggccgtt ccggaccgca aggccatgat cgagcgcaac
     3961 cccgcggtgg ttttctgccc ctcggacatg gacttcgccc agggggagcc cgagcgccgc
     4021 cgcttcttcc tggaccagac cgccagcctc gtgtccccgg gttacctgga cagcctccgg
     4081 gcctaccgca aggtccttcg gatgaggaac cagtccctca aggacggcag gctggacgtc
     4141 ctggaggtgc tggacgagca gctggccgag tacggaacgg tcctccggga tcggcgcgcg
     4201 gagatcctgg ggctcttcga cccggtgttc gccgagcgct acgaggcggt ggctcggctg
     4261 gggatccggg tgggtctgga ataccggccc tcctggaagc cgggaaccgg tccggaggag
     4321 gtcgtcgcgg ctctccgtgc gcggcgacgg gacgagctgg ccctgggggt gaccctctcg
     4381 ggaccgcacc gggaccggtt ccggttcacc cgggaggggg gggatttcgc cgccttcgcg
     4441 tccacgggac agctgcgcct cctggccctg accctgcgcg gcgcccaagc ggggttctgc
     4501 gcggagcgga ccgggaggaa gccccttctc ctgttggacg acgtcctgct ggagttggac
     4561 ccggaaaagc gccggcggtt catggagggc cttccgcccc acgggcaggc tttcttcacc
     4621 ttccttcccg gggaacccta cggggactac gcggacgatt ccacgatggt atactgggtg
     4681 gacgatggac gactctcgga taaggaccgc cgctgagctc atctccgccc tgatctcccc
     4741 gaaggccgcg gaagccgcgg ggacctgggc caaggtcacg ggagcctggc ccgccctggc
     4801 gggggaccgg gcggctcccc attcccggat cgcggacctg gagaaccacg tggtcaccgt
     4861 ggaggtcgac catccggggt ggatccagct cctccagttc cggcaggccc ggctccttac
     4921 ggagatccag gagcggttcc cgtccctgga ggtccgggcc ctccagttcc gtctggcccg
     4981 gcggggcggt cccgggccgg ccggtgcggc cgacggggct ggccggggcg gctcgtccgg
     5041 agataccgga cggcccgccg atcggaacga tgcgggtccg gggaccgtgg cggggcatcc
     5101 cgagggtccg gagagcccgc cgcccgccgc ccccctacgg ctggatccgg aggccgtcgg
     5161 ggatgaggcg cttcggaaag ccctttcggg cctccgggac gcctacgaag gccggtgaag
     5221 cgcggtccgt tgaccaaggg agccgaaacc cgtataatcc ccaagcttat accgagaatc
     5281 cgaaggcggt ccggcctgga gccgaagctc ccccggaaag cctgccaata gcaaggagca
     5341 gttcgccatg aaacgcacgt accagcccag ccggacccgc cgggtccgca agttcggctt
     5401 ccgggcccgc atgaagaccg tgggcggccg cctcgtcctc aagcgccgcc gggccaaggg
     5461 gcgcatcaag ctgaccattt ccgacgaagg caagaagtac tgatcccgtg gggaacgatg
     5521 ccggcagggc gcggcgctgt accttccgga agcgcgaacg cctgcgcgaa cgatcggaca
     5581 tagacagggc tttcaagcag ggaacccggt actcggtgag gggcatgcgc ctccacgtcg
     5641 ttccgaacgg aacggccgcc gtccgggccc tcttcgttcc cgtgcgaaag tacgggaacg
     5701 cggtcctgcg caaccgggcc cggcgcctgg cttccgaggc ctaccgcctc gtgaaatccg
     5761 gagtcgcccc gggccgcgac ctggtcttcg tcctgtaccc cggcaccgat tccttcgaaa
     5821 cacgatcggg tcaggtggag cgggtgctcc gcctggcggg cgctatgccc gggggatcat
     5881 gcgagctctc ctctcggcgg tcttgatcgg cctcatccgg ttctaccggg ccgtcatctc
     5941 tccgctcaaa ccgccgtcct gccgcttcta tcccacctgt tcggaatacg cgctggaagc
     6001 cgtacgcaag cacggtccct ggaaggggac cggattggcg gtacgccgga tcctgcgctg
     6061 tcaccccatg aaccccggag gctttgaccc agtaccatga tgaacgaaac ccgatccgac
     6121 aaggcgttcg attccaagac catcctggcc atcgtcctgt ccgtcctcgt gatggcggga
     6181 gggacctgga tccagcagaa gttctggccc acccagcccg cccccgcggc cccgcccgcg
     6241 gcgacccagc cggccgccgc ccagcccgcg accgcaccgg ccgccgtctc cccggggctt
     6301 ccgacgcccg gcgccgccgc tcccgtactc gcggccgcgg agttcgaggc tccctccgcg
     6361 gaggcgacct acaccgtcaa gaccgacgtg atggaggcgg tgttcacgaa ccggggagga
     6421 gagctcgtct ccctgcggct gcccgagcac gccgacaagg acggggtcgt cgagctgatc
     6481 gacgcccccc ggggggaggc ccaggccttc gccctggccc tgggcgggcc caacgccccg
     6541 cccctccggg agctcatgaa cttccgccgg atcgacgacc ggaccgtgga gttctggagg
     6601 accttcctgc ccgcagccgg gggaaccgac cccttcaccc tccggaagcg cttcaccttc
     6661 gtccccgggg agtacatgtt ccgcctggag atcgtcctgg agaactcggt gaacgacgtc
     6721 ctgcccctga ctacggacgg tttcgcctac accctgggct acggtccccg gatcggaccg
     6781 acgatccacg agggcggacg ctacgcggac ttccgcaagt tcgcggccct ggaaaacggc
     6841 aagaagaaga acatcaaccc caagggcggc gtggaaacca cggaccgccg ggccgcctgg
     6901 agcgccgtgg cgggcaagta cttcaccctg atcgtcctgc ccgacgccac tccgtacacg
     6961 accacctacc ggaaccacca ggagaacggc aagacggacc tgtcttccct ggccttctcc
     7021 cggcccgtcg tcaaggccag ccggcagacg gacatcttcc atatctactt cgggcccaaa
     7081 tcctcccggg agctggtgcg ctacgacgac tcctccaaga acgccttcgg ccgcaccggg
     7141 gacaagctgg aggaggtcat ggaggggggc aacgtcctgg gctggctgga ggccatcctc
     7201 aaggtgggcc tgaacttctt ccacggcctg atcccgaact acgggatcgc gatcatcctc
     7261 ctgaccatcc tgaccaaggt cctgatgtac ccgatcacgg ccaagggctc ccagggcacg
     7321 gcccgcatgc aggagctcca gcccaagatc caggagatcc aggccaagta caagaacaac
     7381 ccccagaaga tgaaccagga gatggcggag ttctacaagc gggagggcta caacccgatg
     7441 tcgggctgcc tgcccctgct gatccagttc cccatcttca tcgccatgta caacctgttc
     7501 aacaaccact tcgacctgcg gggagccagc ttcatcgcgg gctggatctc cgacctctcc
     7561 cagcccgagg cgatcttcaa gttcgccgag ccgatcaacc tggtgatctg gaagatgagc
     7621 gccatccggg gccttcccat catctacgtg gccagccagc tgttgtacgg gaagttcacc
     7681 cagcagcccc agtcgggcgg gcagagcgcc agccagatga agatcatgat gtacggcatg
     7741 ccgatcatgt tcttcttcat cctgtacgac gttccctccg gtctcctggt ctactggatc
     7801 gtatccaacg tcctcaccat cggccagcag gcggtcatca accggatgat ccaccagaag
     7861 aagctggccg cggcggccgc cgctcccgcc cccgtcatcg ccccggtcaa gggccggaag
     7921 ggcaagggga ggtaggatca tggtgtacga attcgaggga aggaccgaga aggaagcgat
     7981 cgacgtggcc gccgcggaac tcggcctggc ccgggacgag ttcgacgtcg aaattctgga
     8041 aacccagtcc ggcggactct tcaagaaggg caaggtccgg ctccggatcc acgtccattc
     8101 caacgcccgc ccggcgccga tctcggaggc cgcggccgcc caggccgcgg gccggccgat
     8161 ccccgagccc cggtacgacc gggaaggccg cggcggacgc cgcgaccgcg gcgaggaacg
     8221 cgccgaggag ccccgcacgg cggcgccccg ggccgcggcg acggatcccg cgggggccga
     8281 cgagttcgag gccaaggtcc tggagttcgt gaagaccacc gtggagaaga tgggctacgc
     8341 ctgcgaggtg tctgtcctct tccgggagga caagaagctg ggggtccggc tggacagcga
     8401 gcacgcctcg atcctgatcg gcaagaaggg caagaacctg gacgccctcc agctcctggt
     8461 caacgtctac ggcggccgtc tggggcggga ggatctccgg atcgtcatcg attccgagaa
     8521 ctaccgcatc cggcgggagg aagcccttgt caagctggcc tacgagtccg cggaaaaggt
     8581 ccgccggtcc cggggctccc tgctcctgga accgatgaac cccttcgagc ggcgcatcat
     8641 ccacaccacc ctgaacgaca tcgtggacgt ggagaccaag agcgaaggcg agggcctcta
     8701 taagcaggtc cgggttctct ggaagggagc caaacgctga ttcggggtcg gtaggctcga
     8761 atttctcccg aacctgcgtt accattacgc cgaggggcgg gacagtcgta cggaatccgc
     8821 cctccttcgg cttggttagg gggattccga tgggcatcca gtccgcgatc cgaaaacgga
     8881 agagtccgac ggtcccggtt cccggggaag cgggaagccg gggctccgga cgcacggcct
     8941 tcgacgagct ctcccgggcc tccgccctcc tctcctccgg gacggatttc ggggagctcg
     9001 agtccatcct ggtcgagcag gcctgcgaca tcacccggtc cgacctggcc gccctctatc
     9061 tctacaccgg cgatccccgg gaccccaaga ctcccctccg gctctcctac aagcggggtc
     9121 cctggtccgt gcccgcctcc ctggcccgat ccggggaggc gctctcgttt ctggaagact
     9181 gcggggaagc cgcggtcctg aaccgccgcc gcccctgcgc cctggagggc atactcctgc
     9241 acccggagat ggcctcgggc atggccctgc ccgtggcggt gcccaaggcc gccgtcggga
     9301 tcctgttcgt gaacgcccgg accgaaaacc actatgaccg gatccggctg aacttcctgg
     9361 gatccttctg cgccctggcc gcggggatgc tccggagctc ccggcttttc caggagctca
     9421 aggagcacgt cgccgtcata gaatccctgg aacgctacca ggagagcgtc ttctcctcca
     9481 tgacgaatct cctcgtgacc accgagcggg acggctcggt ccggtacttc aacgaggagg
     9541 ccgcccgggc cttcggactc tccgaggagg acgtgggccg gcccctgaac gacctgctgg
     9601 gtacccgcgt ggacgccaag atcctccgta ccgtggagga gaccctctcc aagggatcca
     9661 ccatcctggg ggtcgagggg atcgcggcgg gagcccgggg ggatctggac ttctccctga
     9721 acctctcccc catccgcggg aaacggtccg accggcacga cgggctcacc ctcctcttca
     9781 cggaccagag ccgggaacgg gccctcaagg agcggatgaa cgtcgtggtc gaggagcgac
     9841 gggccatcaa ggacatgttc agccgctacc tctccagcga ggtggtccag cacctcatgg
     9901 aatccccgga ccaggtacgg ctcgggggcg cgggcaagaa ggccacggtc ttcttcgcgg
     9961 acatccgggg ctacacctcc ttctccgagg gccgcacccc ggaatacatc gtggaagtgc
    10021 tgaacgacta tttttcccag gccgtggagg tggtcctcca gtacaagggc ttcatcgaca
    10081 agttcatcgg ggactgcatc atggccgcct ggggggtgcc catgaccagc gaggccgagg
    10141 acgcggtgaa cgccgtcacc tgcgccctgg agatccagaa gctgatcgag gacccgaagc
    10201 gtaccttctt ccggggggac gccgagcacc tacgggtggg catcggcatg cacaccgggt
    10261 acctggtggc cggcaacctg ggctcctccc ggaggatgaa ctattccgtg atcggggaca
    10321 cggtgaacgt ggcggcccgc ctggagggcg tggccaaggc gggggaggtg atcatcaccg
    10381 aggacacccg cggtctcctg ggaagcgcct tccagctgga ggagcgggaa cccgtcaagg
    10441 tcaagggcaa ggtcgaaccc atccatatct acagggccgt aaaacgccgc tagagtccgg
    10501 aggtacagca tggaacgtat cgtcggtttc ctggcaggga tatccacggg aacctggatc
    10561 gcggcgggaa tcgcggccgc cgtagccgcg gccgcggtcc tgatcctctc cgcccaggcc
    10621 aggttccgcc gcttcgtgcg gaccgcggcc ctggaccccg cggccctggg cggccgcagg
    10681 ctttctccgg gaaccctcgc gctccgggaa cggtacgtga tccggcgcgc ccgcgggggc
    10741 ttccggggga atcccggccc cttcccccgg ctgcccgcga ccctgggcta cggggagcgg
    10801 tggatgtccg ccctggagtc cgggggcggc cgcggcacga tgtcccgggt cctggaattc
    10861 ctgccggaca cgggtctctt cgcgtgtttc cgggcggccc tccgcaggga agccctgggc
    10921 cggcagctcc tggagtggac ggggacggac gcgggggcct tcgccctgcg caggatcgcc
    10981 ctgtcgggcc cgggccggga cttcgacggc ccgaaggcac gtgcccttct ggcggaacgc
    11041 ctggacgagg tgcgggagct cctgggcgat cccgagtggg cggtccgggt cttcgcggtc
    11101 cggatcctcc tgggggacgg cgaggagcgg accgcccgga gcctccgctc ctgcctggcg
    11161 gatccccatc ccctggtccg ccgcctgatc gtcgaatcct ggacggaccc ggaccggaag
    11221 gggttctacg aggggctgta cgggctgctc gtgtccgatt ccgtgctgga ggtgcggaaa
    11281 gccgcccggg cgcggatccg gaaggacttc gcggacctct attccccgga tccgggctcc
    11341 ctgggtcccg agcaggtcct ccatttcctg gagctcctgg atcccgcgga tccctcggac
    11401 gaggggatag ccttcaagta cctggaagcg gactccccgg aggaacggct cgcggccgcc
    11461 gagtgcctcc aggcgggcgg ggccctggcc cgggtcctgg agaccgcgga gctcggggac
    11521 gccgtggagc tggagcgccg gttccggctc ctggacgccg cggcggaact ccaggtgacc
    11581 ggcttccttt ccaaggtcga gaccatcgag ggcgacgggg ccttccacct ggccgcccgt
    11641 atcctggccc gggccgggga ccggaagcac atcgcgaccc tcgccaagag atggttctcc
    11701 cggatgggca tgccccccta tactccggaa cgtctgggca tctacgacct cgtcctggcg
    11761 gcttcccggg ctcggggcgg cgaggacgcc tgcgagctcg tggtgggcga gctgaacgcc
    11821 cggaggggcc agccggaact ggcctccaat atcctcatgt ccctggaggg caaccagggt
    11881 ccctgcgtgt tcccggccct gcagggcctc ttcctggatc cgaccttcga actccgggcc
    11941 cagctgcggg cggccctcct gggccagccc cgggagcagg tggttcccct ggccctgtcc
    12001 ctggtgaggg cggaccgcgt ctccctgccc cgggtggtcc ggaaggacgc cctctacctg
    12061 atcggggagc tgaagctgac cggggccctc cagcgggccc tggagtccct tcccctcctg
    12121 gacgcggacg agatttccga cttcgccccg gtgctggcgg gggcggacat gaaggccttc
    12181 gagcggaagg cccgggtgat cctggacggg gcggacgccc cgacccgggc ggcggtcatt
    12241 gccgccctgc ccgccgcggg atccaaggcc ttcctggcgg acgtcaaggc ggccctccgg
    12301 gacgcggacc cggacatccg ggtggcggcc gcacgggccc tggcgggctt ccacgagggc
    12361 aaggccctgg cgtccggggg gctcgacctc ctgcgggatc ccgtggagcg ggtccgcttc
    12421 gccgcgtccg cggccctggc catggaggga ggggccgcgg tcctgcaggg cctccgctcc
    12481 gtgctggacg accccaacga ggtggacgac gtcaagcgct ccctgatcga gggcctgggc
    12541 cgggcggatg acgtcggcag cctggacctt ctggcggaca tcctgggcga gaaggaaggg
    12601 caccgggagg agatcttcaa ggccctggcg gcgcgggtgt ccaagcgcga cctggagcgc
    12661 ctggtggaga ggttcaagga cgccacgggt gacctcaaga ccctcctttc cgaggccttc
    12721 cgccgcatgg gcgagacggg ggagcggacg atggcggccc tcctggagga ggacatcgcc
    12781 tccctcaagc cctacatcgt ccaggtcctg gaatcccggg gttacgtgga ggcccggatc
    12841 ctggagctca agcaccggga tcccaaggtc cgccgggacg cggccgccgc cctgtccaac
    12901 gtgggaagcc tgcaagcctt ccggggcatc gtcctggcgg cccgggatcc cgatcccgag
    12961 gtgcgggtgg ccgtgacccg ggccctggag aggctggcgg gtcccgaggg agcctccctc
    13021 ctggcggagc tggaggcgga cccgaatccg agggtgcgca agtacgtgct gtgggccctg
    13081 gaacgggtca aggcgaaaaa cctgtgagaa gcctatacgg ggagcctgcc gtgaagaatc
    13141 gtaagagtgc gctcatcctg gtcctgatcc tgctggccgc gcccgcctgg gcccagctac
    13201 agaaaccgac cgccgaggtc cggcgcttcc aggtggaggc cctgaccctg cgggacgtga
    13261 ccttcctgtt cgagctggcc gtgaagaatc cctaccccgt ggaactgtcc ttttcgggaa
    13321 tgaccctgga cttctccgtg gagggagcca aggtcttctc cgcccagagc aaggggggat
    13381 tcaaggtccc ggcccgccag gagaaggcga acaccttcac ggtcaccctg gagtacgagg
    13441 cggtcatccg gatggtcaag gagtacgcgt ccaaggagtg gctgaacacc gtgatcgacg
    13501 gggtcctggt catccccctg ccgaagcttc cgggcctgcc ccaggacgtg aaattcacgt
    13561 acaagctgga gaagaagatc cccgcgatca agccccaggt ggccctggtc gggttctcgg
    13621 tcgagccccc cacggccgcc caggtgaccc aggccctggc caaggcgggg aagaaagcgg
    13681 atcccgccaa ggcccagaag gtcttcgcgg acgtcctggc cggcaggaag cccgaggccc
    13741 cggtcatcga tcccgcggaa ctggaccttc ccctctccgt tagtttcacc ctggagatcc
    13801 ggaacgacgc caaggggccc ctgtccttcc agaagctggg gtacgagctt ttcgtgaacg
    13861 gcgagtccct ggtcctggga gacagctccg gcgtcgtgcg ggagggcgcc cggaccctgg
    13921 tgaaggtgac caacaccttc agttccaaga agctgtcgaa gggcgtccgg gcgatcttct
    13981 cggaccggaa gggaagcttc gcggtcaagg gcagcgcctc gatcaagctt ccggacgaga
    14041 ttcggaagga acccgtgccc ctggccttcc acgaaacggg gacgttctcg ttgaaataga
    14101 gaaggggctg tccaaaaagc gcagctcgct tttttggaca gcccgtccct cgataaaaca
    14161 gggcgggccg gcaggtccgc ccgtttccga tcaggccagg ttgaacatct tcctcagggt
    14221 ttccacgttc cgaggattcg ccggccggga gccctcggat ccgtagaggt aggcgatccc
    14281 ctcggcgtgg accttggcca cccggtggcg gaccagcttc atggtcgagt tcaccgtccc
    14341 gtccttgtca ctgaagccct cgggcacgat caggaaggtg gcggggatcc agttggcggg
    14401 gactttcttg gccttggggt cggacttgaa gcggtagaac tcctcggcca gccggtccat
    14461 cagggcggcg gcggtcttga tcccctcggc cttgatcatg cgttccacgg ggccggtctc
    14521 cagggagacg atgcccgccg tgtacttgct ctgctcgttg taggccatga tctgggcgaa
    14581 gcagtccgtg gaggtgccga tggcctcctc tatctcctcg gggctgtact tctccccgtc
    14641 cgccgcgatg aggagggcct tctcccggcc caccaccacc aggaacccgt ccttgtcgta
    14701 gtaggccagg tcaccggtgt acagggcccc gtcccggagg gccttggcgg aggcctcggg
    14761 gttgcggaag tagcccttca tgacgttctc gccccggatt acgatctcgc cggtctctcc
    14821 gaccgcgcac tccgtgccgt cctcccggag gatgcggcac cggacggtgg gggccacgat
    14881 gccggaggtg ccgaacttgt gcttgcttgg ggtgttggag gagatgacgg gggcggcttc
    14941 cgttaggccg tagccctgga agatgggaac cccgaaggcc gcgaaaaact cctgctgctt
    15001 gacgtccagg agggcgccgc cccccacgca gaagcggatg tttttgccga aaaccatctt
    15061 cttgatcttg ttgaagatca gggccttggc gatcccgtgg accagggcgt tcctggcccg
    15121 gacgccgaag ggcacggggt tgaatccgtt cccgttgacg gcgatccccg ccgcgatccc
    15181 ggccttgaag agcttttcta tgaagccgcc cttttcctcg atccccgcca cgatcttctt
    15241 catgaagttc cccgaaaggg ccgggacggt gagaaggaac atgggttcca cttccttgag
    15301 gttgatcggg atgttgcgca ggatggcgat cccgccgccc cgggggtcca cgaagtagag
    15361 ggagattccg cagagcaggg cggcgtagat gcccacggtg tgggcgaagg agtggtccac
    15421 gggcaggatg attagggtgg tgaagaagta gggcttctcg aagagctcga tcccgtcctt
    15481 gcagttggcc cagtagttga ggtgggtcag catgatgccc ttggggtcgc cggtggtccc
    15541 gctggtatag cagatcgtga ccgtgtcgtc ctcggccgtc gcgtcctcaa tgtccttgag
    15601 ggcgggggtg cgggcgggga gggcggtccg accctcctgg agggcatcct tgaagcggac
    15661 gatgttcttg cgggggaatc cggtcttgcc gcagaccgcg tcgggccgtt cctcatcctc
    15721 gtccaggtag accacgaggg tcttcttggc gtccgtggac acgagggcgg tcaggacctt
    15781 ctccagctgg ttgcccgttg tgatgaaaac cttggagccc gagtgcttta cccggaaggg
    15841 gatctcctcg gacaggagct tgatggacag gggcacggag atccccccgg ccatgagcac
    15901 cccgaactcg gcggccaccc attccgggct gccctcggcg aggatgcagg cggtatcctg
    15961 ccgtttcatg tcccggccga ggaggaagga ggcgaaggca cgggcttcgg agcgcacgcg
    16021 gccgaaggtc cagccttccc agccggcgtc ggtcttttta aggacgtagt tgacgttcgg
    16081 ataggcgcgt tcggcctctt ccagcatgga taacacggtc cgtttcatcg tccctccttg
    16141 ggtatcacac cacactacca tgaaggtacg ttccgcggaa gagcggatct attgacaacg
    16201 tctccggggg ggtgtagttt caaggcatcc tctccccaag gcgaatccaa tgaactactc
    16261 cgaccccacg agactcggga tcctggcctg tccgggcggc gagcgcttca cggaactggt
    16321 tctccagcgc ctggcgtcga tctacaagcg caggttcgag cgaaagaccc gggtcctggc
    16381 ggaccggtac cacctgtccg cggagctggt catccggaag agcaatttcg ccaacgacat
    16441 ccagtcctcc atgctctaca tacccgggga cgtgaacaag ttccgggccc cccggttcaa
    16501 gatcaacgcc cgccactcct ggttcgccaa cggggagatc aagacggaga tcctggattc
    16561 cgtgcgcggc aaggacctgt acatcgtcca ggacgtggag aatcaccaac cggtcaagtt
    16621 caacgagggg cgggacgagc gggtcctctc ggtcaacgac cacatcttca acctcctggt
    16681 ggccgtggac gcggccatgc agtccggggc cgagcgcatc acggtggtcg ttcccacgta
    16741 tccctattcc cggcagcaca agaagaaggg gcgggaaggg ctcaccgccg cgcggatagg
    16801 gcagatcctg gagaacatgg gggtgtcccg gatcatcacg ttggacatcc attcccggga
    16861 gatcgagaac tgcttcaaca agctccggct cgaaaacctc cacgccagct accaggtgat
    16921 ccagaaactc gcgaccgtcg tggacgtgaa ctgcgacgac ctggtggtcc tgtcccccga
    16981 cacgggggcc atcgaccgca acaagttcta cgccagcacc cttcagaagc ccctggccct
    17041 gatctacaag gaacgggact actcccgggt cacgaaaaac gccgaggaat ccaacatcac
    17101 ggacatgaag ctcctggggg acatccggga caagatcgtg ttcatggcgg acgacatgct
    17161 gggcaccggg gggaccctcc tcaaggccat gcagttcctg agggaccagg gagcccggca
    17221 ggtgatctgc gccgtgtccc tgcccatgtt ctcggccacg gccatccagg acttcgacgc
    17281 ggcgtaccgg gagggccggt tccaccgcat catcgggacg aacgccatcc accacgacga
    17341 gctcttgaag aaggagtggt acgttcaggc ggacgtttcg accctcttcg cccagatcat
    17401 ctccctggtg taccacaacc attccctctc gcccatgctg gacaaccggg gcctgatctc
    17461 caagatgctc aagaagtccc agcaggcggc ttgcccgccc caggggaacc tcttccagga
    17521 atgagggttt tccgccttcc cgccgctccc cgggccctcc tgttcgacct cgattccacc
    17581 ctatacaccc acgcggagta cgcggccttc cagaacgagg tcctggtggg cgagcttgcc
    17641 cgacgccggg gcgtcgacga ggagcggatc cgagccgaga tccgggcagt ccgggaccgc
    17701 gtggctcggg agaccgggaa ggtcacctcc ctgggaaacg ccatggccgc ccttggggtg
    17761 gacatcccga ccagcgtcgc ctggagggaa cggctgataa aaccggggga ctggctgacc
    17821 cgggatgatc gactggccga agccctgtcg gccctgtcgg cgcggttcgt cctggcggtg
    17881 gtgacgaaca atccccggtc cgtgggccgg gagaccctcc gtgtgttggg agtggaggat
    17941 tcattccggg aggtggtggg cctggacgac acgatgatct ccaagcccgc ccgggaaccc
    18001 ttcgagttgg cggcccgtcg gctgggggtg cccgccggag actgcgtgtc cgtgggggac
    18061 cgctacgacg tggacctggc cgtgcccctg gaactgggga tgggcgcggt cctcgtggac
    18121 ggggccgagg acgtctaccg cctgccggaa ctgttcaaga cggcgggcaa gccgaaagac
    18181 ggacggtttc ctgatctaac cacggagatc acggagagca cggagtgaga ataatgaaat
    18241 agaaggaggg gcgatattca atcctagttc ttttctatat taagattgaa gttacattga
    18301 aatccccatc gctccttttc cctgctccgt gtcctccgtg ttcccagcgg ttcctgaaac
    18361 ctcgacgaag cccctcgggg cttcgttgcc gtgaaatggc cggccacggc cggccatttc
    18421 acgctacttc gcgataaggc cgctgatcat gcccccggcc acctcgatgc aggttgcatt
    18481 gacggcgtcg ttggacacga tgaaggcgat tgtctcggcg atctcctcgg gttcgatgag
    18541 gcggccgagg tgcacgccgg aaaggatctt ggccagggcg ccctggtcca tgcccttgac
    18601 catgggggtg ccgacgtaac ccggggcgat ggaggccacc cggatgcccg tgattccctt
    18661 catctggaac tcgccgacca ggatcttggg ccacagggcc atggcggcct tcgtggagga
    18721 gtagttgatc tgccccaccc ctccctgctt ctggatggag gagatagtta tgaggacgcc
    18781 cttccacccg ttgtcgacca tgcgggaggc ggcttcccgg atcgtgatga agggacccac
    18841 caggttcacg tcgatgacgg cccgaaagtc cgcggtggac aggacccgct tgacctttcc
    18901 ggtttccttg tccggggaga tcatgaggcc gtccttgatg atgccggcgc aggggaccac
    18961 cacgttgatg gccccgaact ccttgaccgc gaagtccatg aaggcggccg tgtccgcgtc
    19021 gtcggtcacg ttcagggtct tgcccgcggc cttgccgccg gccttcttga tctccgcgac
    19081 cacccggtcg attccggcct ggttcatgtc ccctatgacg acggacgcgc ccttggcggc
    19141 cagggtcttg gcgaccgctt ccccgatccc gctggccccg ccggtgacga cggcgaccga
    19201 tcccttgatt tccatggagt ccctcctgaa gaaaatgacc atggtcaatg accacgtttg
    19261 ttgactgcgg tcattgtaac gccctttttc ccgaattgtc aaaaaaaatc ttgatctttc
    19321 cggtattccg gattatacag tacttggacc ggacgaaccc acctgacttc ctcgaagccg
    19381 cgcgcggggc gcgtacgccg cccgccgcaa gcactgcgga tgaaggagtc acaatgaaaa
    19441 gcgacaagat tatgagcgct gcggacgccg tggcccgcat caagagcggg gccgtggtcg
    19501 ccaccgaggg attcgtcgga aacggattcc ccgaggagtt ggccgtggcg ctggagcaga
    19561 ggttcaagga gaccggggcc ccccgggacc tgactctgat ctacgcggcg ggccaggggg
    19621 acggaaagac ccggggcttg aaccatttcg cccacgaggg gctggtgaag agggtcatcg
    19681 ggggccactg gggcctggcg cccgccctgg gcaagatggc catggaaaat aagatcgagg
    19741 cctacaacct gccccagggc tgcgtctccc acctgttccg cgccatcgcg ggcaagcgcc
    19801 cggggcacat cacccacgtg ggcctcaaga ccttcgtgga cccccggaac ggcggcggga
    19861 agatcaacgc caagactacg gagaacatcg tcgagctcct gaccctggac ggcaaggagt
    19921 acctccgcta ccggaccttc ccgatcgacg cggccctgat ccggggcaca tccgcggact
    19981 ccaaggggaa catctccctg gagaaggaag ccctgtacct cgagaacctg gccatcgccc
    20041 aggcggtcca caattccggc ggcgtggtca tcgcccaggt cgaacgtgtc gtggaggacg
    20101 gcttcctgaa ccccaaggac gtggtggttc cgggcatcct ggtggactgc gtggtcgtgg
    20161 ccaagccgga aaaccactgg caaaccttcg ccacccagta caacgccgcc tacgcctccg
    20221 aagtccgggt tcccgtggcc tccctcaagc ccatggagat gtccgagcgc aagatcatca
    20281 gccgcagggc tgccatggag ctccacccgg gcaccatcgt gaacctgggg atcggcatgc
    20341 ccgagggggt ggccatggtg gccgccgagg agggcatcct gcacgatttc accctgaccg
    20401 tggagcccgg gcccatcggg ggggttcccg cggggggtct ggatttcggg gccgcgacca
    20461 acgcggaatg tctggtggac cagccgtccc agttcgattt ctacgacggg ggcggcctgg
    20521 acctggcttt cctcgggctc gcgcaggtgg acgagcaggg caacctgaac gtctccaagt
    20581 tcggccccaa gttcgcgggg gccggggggt tcatcaatat cacccagaac gccaagaagg
    20641 tcttcttctt ggggaccctg acggcgggag gcctcgaggt ggcggtggag ggcggaaagc
    20701 tcaaaatcgt caaggaaggg gaggtcaaga agttcgtgaa ggctgtggaa cacaagacct
    20761 tcagcgggga atacgccgcc caggtcgggc agacggtgat gtacatcacc gagcgggccg
    20821 tgttcaccct gaccaaggaa ggcctcgagc tgaccgagat cgcacccggt gtggatctga
    20881 agaaggacgt gctggacctt ctggagttca agcccgtcgt tcgaggcacc cctaagctga
    20941 tggattcgag gatcttcgcg gacaagccca tggggctgtc gaagaactag ttcggacatc
    21001 cgtctctgat tccaactcgg gctccggcaa ggaattatcc ctgccggggc cttttttggt
    21061 ttcttgatac tatactatga atcatgatat gctccggatc cggagggttc atgaccaagc
    21121 gtacgaacaa gcggcaggaa caggcggcgt ccacgaaggc cgcgctgttc gagagtgcca
    21181 tcaccctctt caacgaaaaa ggctactact ccgtcacaat cgaggagatc gcacagcggg
    21241 ccggtaccgc aaagggcagc ttctatacct atttccggac caagagcgac atcatcatcg
    21301 aggaattccg ggccatcgac gactactaca agcgcttcga gaagaccctt ccccggtttc
    21361 cgaacgccct ggagaagatc tacgccttca acagggccca gatgaagtac gtccgggacc
    21421 aggtgggcct cgagatgctg aagatcctgt atgccaacaa catcctcgaa cccagcacgg
    21481 aaaagatcct gatcaacacg aaccggtatc ttcacggcct catccgggga ctcatcgagg
    21541 aaggtcagcg ggaggggctc ttccggaccg acgtgtccgc ggaccgcttg gccctcctgt
    21601 acacccgagg ccaccgggcg gtcttcctgg actgggcgat ctccaacaac gccttcgacc
    21661 tggtcaagga cggcgtcgag ttctgcaaga tcgtcatgtg ccctgccttg atggccaagt
    21721 ccgtcccggc gaccggcacc gactcggaac cggacaagta gcggttctgt atatcttttt
    21781 atccacttct tcttaaaagc cccgaaatga agcccggtcg ataagttttt attgactgcg
    21841 gtcatcgaaa ttaggggact cgcaagaaat ttgttgacag attccgggac ccgcgatacc
    21901 gtcgagtgac cggggccatt gaccatggtc aatgaccgcg gacaattcaa cacggaggcg
    21961 gtaatgagca aggtctcgat cgtcggcgcc tataacacca aattcggttc cttcgtcaac
    22021 aagaacaagg agacggggga aatcaccgac acctgctccc tctacgagct gatggccgag
    22081 gccgcccagg gcgccgtcaa ggacgccggg ctggatcccg cccagatcga cggagtctgg
    22141 atcgggtcct tcgcgcccgg actcttcaca agccaggacc acctggggcc cctggccctg
    22201 gagtccttcc cggacgcctt ccggttcaaa cccacccaac gggtggaggg ggcctgcgcc
    22261 tcctcctcca tggccgtcta caacgccctg tacgccatcg agagcggccg gttccgaaac
    22321 gtcctggtgg tcggggtgga gaagatgaac ctcctcaaga ccgccggcgt gacccacgcc
    22381 ctggccgggg cttcctactg gccaaccgag ggcggcgtgg ggatgacctt ccccgggctg
    22441 ttcgcggaat atgcgaaggg ctacgcgaag cagtacgggc tgtcccccga aaaactccgg
    22501 agcatcctgg cggcggtggc cgccctctgc tacaagaacg gcctcgagaa tccgctggcc
    22561 catttcggca agggcgggcc ctcggacaag ctgggtctcc acacggcggc caacatcctg
    22621 gggcttccgg acgagaagaa tcccatggtc gccgagcccc tgcgcctcca cgactgctcc
    22681 ctggtgaccg acggggccgc ggccctggtc ctgagcccca cggaggaggc caagcggagg
    22741 gccggcccgg cggtggagat cgcgggcatc ggccacacca ccgagcgcat ggccataaag
    22801 gaccgggtca acatgtacga cctgatcgcc gccaagaaag ccgtccaggc ggcctacaag
    22861 gaggccggaa tcagcgccaa ggacctgagg gccgcggagg tccacgactg cttcacgatc
    22921 acccaactcc tgtgcaccga ggccctgggc ctctccgggg acgggaagtc gggcttcgag
    22981 tacgaggccg gccgcttcgg gcccggggac gactgcccgg tcaacctctc cggcgggctc
    23041 aaggccaagg ggcatcccgt cggcgccacg ggggcgtcca tgcacgtcct cctctataaa
    23101 cagatgacgg gcaagcccat cggccaggcc cgcgagaagg gggctcccga ggtgaccgcc
    23161 accctgaacg tgggagggtc cgcggtgacc aacgcggtca gcgtgcttcg aaggatacgg
    23221 taagaaaggc ggccggccga gtcgggagac gaagccggcc gctttgagga taaagacaag
    23281 gagggaaccg gaacagcaag aggaaggagg gcacggcccc tgcaaggcgc ggagtgcgtc
    23341 cggtccggga caagcgctcc aagttgtgga tgcgaaatcg aatcctgcct gtcaaaggag
    23401 taccgaacat gttaggagtc atcggtatca tcgtttccct gggacttctg atgtacctgg
    23461 cctaccgggg catctcggtc ctgatcctgg ccccgatcct ggccctcttc gcggccgtct
    23521 tctcggggat gccgctcatc gcgacctaca cccaggtctt catgccgaac ctcgccaact
    23581 acctgaagct gtacttcccc gtgttcatgc tgggggccat tttcgggaag gtgatggagg
    23641 agaccggcgc ggccaaggcc atcgcccacg cgatcgccac caagctgggc aaggacaagg
    23701 ccatcctggc cgtggtcctg acctgcgcgg tcctgaccta cggcggcgtg tccctcttcg
    23761 tggtggtctt cgcggtgtac ccgatcgccg cccacctctt ccgcgaagcc ggaaccccca
    23821 aacggctcat cccgggcgcc atcgccctgg gctcgttcac cttcacgatg acggcattcc
    23881 ccggaacgcc ccagatccag aacgccatcc cgatgccctt cttcaagacc accgtctacg
    23941 cggccccggt cctggggatc atcgcgggca tcatcatgtt cgtgggcggc acctggtggc
    24001 tcatggcccg ggccaagacc ctgaacgcca agggcgaggg ctacggcaac cacccgaacg
    24061 agcagatcaa ggaattcgat tcctccaacc tgcctagcgt gctcgtcgcc ctgatcccga
    24121 tcctcatcgt cctggtgctg aactacatca tcgtcacctt catttacacc gcgaacaacc
    24181 ccgcctacga gtacctcaag gccaaaccct tcgagacgaa catcaattcc gtgcgcggga
    24241 actggggcct catcatcgcc ctcatcgcag gcatcgtcgt ggcgatcctg atgaacctca
    24301 agcgcctcaa ggtcaaggag acggtcaacg cgggagtgtt cggctccatg ctgcccatcc
    24361 tgaacaccgc gtccgaggta gggtacggga acgtcgtgaa gaccctctcg gccttcgcca
    24421 tcgtccaggc cgccctcctg tcgatctccg acaacccgct catcacctcc gcgatcgcca
    24481 ccaacgtcct ggcgggcatc acgggctccg cgtccggcgg catgagcatc gccctgggcg
    24541 ccatgggcga gaccttctac aaccaggcga tcgccaaggg catcaacccc gaggtcctgc
    24601 accgcgtggt ggccatcgcc tgcggcggcc ttgacagcct cccgcacaac ggcgcggtcg
    24661 tgaccctcct gggcatcacc cagatcaccc acaaggaagg ctacgccgac gtgggcatgg
    24721 tctccgtggt catccccgtc ctgacctgcg tggtcgccat catcctcggg agcatcggca
    24781 tcgtataagc tgagcctgag gagttccttc ccgggggagg tccttggaac tcccccgggc
    24841 tttccttatt tgagggagga tcctatgatc ggtaccatcg gagtcgtcgg ggccgggcag
    24901 atggggggcg ggatcgccca ggtctgcgcc caggccggat acgaggtggt cctgttcgac
    24961 gtggtggagg cccagctggc caaggcccgt tcgggcatgg agaagagcct gcggaagctg
    25021 gcggagaagg gcgggatcga tccggccgcc gtggagccgg ccctggcccg ggtcaaaacc
    25081 gtcacggagc tggaggcgat ggcggccgcg gacctggtgg tcgaggcggt caacgagaac
    25141 gaggccttga agctggaaat cttccgcaag ctcgacggga tcctccggcc cgaggccatc
    25201 ctggcctcga acacctcctc catctcgatc accaagctag cctcggccac caagcgtcct
    25261 tccaaggtga tcgggatgca cttcttcaat cccgtacccc ttatgaagct cgtggagatc
    25321 acccgggggc acgagaccgg cgacgagacc tacgcggccg tggcggagct ggcggccaag
    25381 gtgggcaagg agagcgccgt ggtccaggac tatcccggct tcatcgtgaa ccggatcctg
    25441 gtgcccatgc tgaacgaggc cgcctacgcg gtctacgaga acctggccac cccggaggac
    25501 attgacaagg ccatgaagct cgggacgaac cagcccatgg gccccctgac cctggcggac
    25561 ttcatcggac tggacacctg cctggcgatc atggaggtcc tctacgacgg cttcaaggac
    25621 ccgaagtacc ggccgtgtcc gctgctggtc aagatggtgc gggcgggtta tctcggccgg
    25681 aagtcgggcc gcgggttctt cgtgtacggc taggcccgct tcggcggcgc agcgaaggac
    25741 ttccgcagca gggccgcaag ggcggccagg cccgggtccg aggggcccgc gaagcccagg
    25801 tccgcaaggg cgtccgtccg ctcccgggac atccagcggt gcaggcccct ccccgcctcc
    25861 gcggcatccg gattggcgtg ggcgatccgg accaaggcca gcatctcgtc ggcggagaac
    25921 atcttgtacc ggccggcgga caccacgtgg taggtctggc cgtcctggga tcgctcgatc
    25981 cggagggccg gggatcgggg gccggtccgt cctcccgcgg ccgggcgctt cccggcccga
    26041 atcgaaaccg cttccgccct cccggcctcc cgccgttcct ccgcctcgtt caggcgttcc
    26101 acctccatgt tgtaccggtg gacgtgggcc tgctccaaga ggaagtcgag gccctcctcg
    26161 tccagttctt ccaggacgga tttcagttcc ttgagcaggg ccgccttccg cttcttgccg
    26221 gaggcgcgcg ccgcggccga ggctccggcc cgccgcggag gcgatcccga gctaagagaa
    26281 accggttcct ccacggcaga agcccggccc cgtccgcgcg ccggggcgtc ctgctccggg
    26341 gcgggctccg gcgcgggacg ggcccgcgcg gcggcggcgt tcgcggcgga gggtccggct
    26401 tcctcgatct catcccgcag ggccttggat aggcggtcca ggaatttcgg gtccggcttt
    26461 ccgggcgcct tggtcggctt gggtttcctg ggagccagct tcttcttgag ggaggcgagt
    26521 ttcttccggt cagccatggt tcctcctcga gcgccgcgta tagtagtatt ctatccgacg
    26581 gggtccggaa aatccgcggc gggcggaaag ttccgtgtag cggacggcct cgtcacggag
    26641 agcggatgaa ccacgaggaa ttctatgaat cctgccggga ggcccgggag gaactggcgt
    26701 acttcatgcg gcgcctctac gagcgggggc tgacgacctg ctccgggggc aacctgagcg
    26761 cccgcgtcgg ggaagcgcac gtgatcgtca ccccctccgc cctggacaag ggccggatca
    26821 ccggggacca ggtggggctt atcacgatgg acggagaaaa ccggacaccg cacctcaagg
    26881 ccagcatcga gacggggatg cacctggcag cctaccgggc gaggccggac atccgggcca
    26941 tcgtccacgc gcatccggtg accgcaacca gcttcgcggc ccggcccggc ggtatcgaca
    27001 ccaccctgac ggcggaggcc tacacggtgc tgggccgccc cgccgtcgct ccctacgccc
    27061 tcatggggac ccgggacctg gccgaccgcg tggcggaacg cctggcggtc gccgacgtgg
    27121 tgatcctgga gaaccacggg atcctgaccg tcggccggac cctgctttcg gctttcgacc
    27181 ggctggaggt cctggaggcc gcggccaaga tgaccctgta cacggccctc ctgggtggcg
    27241 cggcgcccct gaccccggaa cggctcaccg aactggacgc ctggaaggcc gcccggaaat
    27301 gaagtaccgc cgcggccggt attcggcagg cggcggcacg gtttggggat ggggcggagc
    27361 cggatagact cgaagctagg actttcccgg gtactctatc cccagcctct tcagggtctc
    27421 cggtttcggg atgccctccg gggaccagcc ccggagccgg taatactcgt ccagggaggc
    27481 gtccaggtcc gggggcaggt tgtcccccgc tccccctccc ttgggcacga aacggatgcg
    27541 gtccggcagg gtgtcgtcct tccgggagac ccccagggcg cagttgtagg cgcgcttcag
    27601 gttgaagatc cgctccccgg cggccaggaa ctcttccatg cccatgtccc acccggtggc
    27661 caggttcaac caccccaggg cgacggtcgc gccgactccg ccgttcatgg ccagcttgca
    27721 gagcttcagg ctgtcgaaca ggcccatgag gtcctgggag gcgatgacca gctcggcctt
    27781 gcgttccgtc ccgaaccggt cctgggcggc ggggaagccg cgttcgggaa gggtgatcgc
    27841 tttctcgaag acgtaggtga agccctggag gtggcaggcc ccccggttgc tcgtggcgta
    27901 ggaaagggcc aggctgttga aggcccgggg gtcgtgggcc gggaactcga ggcccttgga
    27961 gtggatggcg tattccggcc tgccgatgac ccgggaggcc cggaggacac cctctccgag
    28021 gagttctccg gctccccggc gctccccgat catccgcacg agctccacca gggcttttcc
    28081 gtcgccccag gcgggcacga cgctccccag gtcgatcccg gcggtctcgc ccttctcgaa
    28141 ggattccatg aggaagccta tggcggctcc cgcggagatc gtgtcgatcc cgaggcggtt
    28201 ggacagctcg ttggcggcga tcaccgcctc cgggtcgccc accaggcagt tggagccgta
    28261 catgcacgcc gtctcgtact ccgggcccgc cccgagggta ccgtccgata gctccacggt
    28321 gcgtccgcag ccgatggggc agcggccgca gaagaagcgc tttttcagcc ggccgctctc
    28381 ggccaggtgc tggccggaca ggggcgcggc ccggtccccg aaactcccct cgttccagtt
    28441 gcggaccggg atgtccccca ggtcctggga gctcacgacg atgcccgcgg tgccgtactt
    28501 caccaggcgc tcggtctttc ccggcatctc ccgggcgaag gccgtgcagg cctccgccag
    28561 ggccgcggag tcgtggacgg ggcgcgggtt cttgtgggcc ggggcggcca caaccgcttt
    28621 caggcgcttg gaacccatca cg
//
LOCUS       DAOTEI010000025        27951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1111_length_27951_cov_5.517386, whole genome shotgun sequence.
ACCESSION   DAOTEI010000025 DAOTEI010000000
VERSION     DAOTEI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 27951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27951
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1111_length_27951_cov_5.517386"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="P5313_04000"
     CDS             complement(<1..181)
                     /locus_tag="P5313_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY79562.1"
                     /translation="MNLRHFLLLSGLLSLPAAHAQPGPDLNALDAYLAKAVTDFDQPG
                     LAVGIVKDGRLIWSKG"
     gene            complement(178..897)
                     /locus_tag="P5313_04005"
     CDS             complement(178..897)
                     /locus_tag="P5313_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY79563.1"
                     /translation="MNTILSGEHIVKSFGDGAERRNVLDGVSVEIRKGEFVSIMGPSG
                     SGKSTLLYALSGMDRVDGGRILFEGRDLSGLRDDDLADLRRRRMGFVFQQPTLLGNLD
                     LLDNIILPAVRDERRGLGRHVEKARDLMRRTGIEGLERRSVSQVSGGQLQRAGICRAL
                     LRDPAVLFGDEPTGALNSASAREILDILSEVNAAGTAVLLVTHDAAVAARAVHHPTAE
                     HGAAASWPHRLVTLAHLQPAP"
     gene            complement(900..3218)
                     /locus_tag="P5313_04010"
     CDS             complement(900..3218)
                     /locus_tag="P5313_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79564.1"
                     /translation="MYARIIRNDIRRSRLASLATVLFVMVSALLVSLAGALGAELSAA
                     LETLMERAATPHLLQMHSGPIDLERLESFAAGSSELEGFQVLEFLNVEGAAFEFGDRT
                     LAGSVQDNGLSIQGRDFDFLLDLDGNVANPREGELYVPVFYFKDGTARAGDRVRILGR
                     EFRVAGFVRDSQMNSALSSSKRFLVSPEDYATLRDRGSVEHLIEFRLRDPGALGNLEA
                     AYVAAGLESNGPRVGFPLFRMMNALSDGLLIVVLLFVGLLVTFVSLLCVRFALLAKIE
                     QDYREIGAMKAVGLRVSDIRRAYLAKYAVLAVPGALFGWALSFAFRGPLLANLRLFMG
                     EGGASRLAPALGLAGALLVGLLVLGYVRGVLGRFRKISAAEALRFGAPRQAARRPGRL
                     RLAAGLPLGANAALGFLDVLARIPLYGTMLAVLVASSFIAVVPRNLHTTVASPSFSSY
                     LGIGACDLRADVQAVGDIPGRASEIASALAADPEVRKTVVLTARAFTVRGKDGSESSL
                     KVELGDHAVFPVAYAEGRAPGAPDEIALSVLSAEDLGTRVGDRLAAAVDGEWRDLRVA
                     GLYSDVTNGGKTAKAAFSGGEAPVLWAVVQAGLADPSRSREKAAAYAAAFPYAKVSSL
                     QEYVAQTFGPTIRAVGSASRISLGVALVLAALITALFVRMLVARDRVSIAALRALGFR
                     NSDLTRQYLARSALVAGIGVLAGSLLANTLGESLVGALISLFGAASFEFAADPLSAYI
                     LIPLAVALAALAATAAGAAGAGRIGIAQTIRE"
     gene            complement(3221..3904)
                     /locus_tag="P5313_04015"
     CDS             complement(3221..3904)
                     /locus_tag="P5313_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79565.1"
                     /translation="MMEAEERRQEILDAAEALFREQGYSGTSTGDILDRTGIARGTLY
                     YHFESKAAVMEALVDRLCDRLFKAARETGEALRDRPALERLLAVIGAFQASGDVGQGL
                     LEHLHDPGNLPLHDRVQRRLVAGMTSILSGIVREGISAGAFDTPYPEESMELVVVYMT
                     EVIDGGLVDLSEAERSGRLRALVFNIGRLFGLGTDDLSRVRSAFGGEPENGTRGSRGR
                     PGKNPKDGR"
     gene            complement(4373..4446)
                     /locus_tag="P5313_04020"
     tRNA            complement(4373..4446)
                     /locus_tag="P5313_04020"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4410..4412),aa:Pro,seq:ggg)
     gene            complement(4551..5420)
                     /locus_tag="P5313_04025"
     CDS             complement(4551..5420)
                     /locus_tag="P5313_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRY79566.1"
                     /translation="MNPPTPRSRAPDLSAFLETAYAELARPELIDPDPLAVTREYVDP
                     GDREVAGLAAACLALGSAPLIVRAAREVLAPLGSSPADALGALGDDDLARLLSGFRYR
                     FFGGEDLAALLSAVRNARARHGSLEVLFRAGDDPSEPTIIRGADRFAAALEDFAGSRW
                     IRVSGKAPWRDNLLPRPSRGSACKRLFLYLRWMVRKDAVDPGGWTGVSPSRLVVPLDT
                     HMGAACRRLGLVTRRADDLKAALEATEAFRRLRPDDPVRYDFALTRPGIHPCLEADAY
                     FRCFDDPEPRPRP"
     gene            complement(5417..7132)
                     /locus_tag="P5313_04030"
     CDS             complement(5417..7132)
                     /locus_tag="P5313_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY79567.1"
                     /translation="MRYRRGPKAAAAALAAAVLGAVLVLTACVSTPPPPDTGTALTMQ
                     GRSGESAAAKTAVETLLARGTPDALSRARELAESSRALSPSDVLLYRWFAVELGRMAY
                     PERLGTVPVAPEPPSGHPWARAFADARTGRIGTPREGADPLELLALASALFRGDSKET
                     TRAATLALELFAQTRLESPLADHLQGVISERSANLSSAQERYVAAFVAAPDCYPALFG
                     AARTLIALGRPQEAVDILSASKTAFGDSFGWVRLMALALYDSGRYDEAGPFVMRVLLE
                     DPLDSRILLIRADMLVRAGDYRQAAPLLDAYAAVDPADRRYLFLRGRVAWEGSRNKDE
                     ALRFFRRLLSIYPDDSETLLAAARILALGTSSERGEAYLMAGRVLAKYPADPGALRIL
                     LAEEIRRRDWTSALATQERLRAADPEYRDRASLYLVYRSAGRFEEAFRVASEWRTASP
                     DSEDARIAYIRSLIDRRDSGGARDLIARALTEKGSARFRSSLYFLQSLVQPNDEAALN
                     SLRSSLVENVQNLEALTAMYDIYMRQKDAQKARFYLRQALAVAPDDPALARRREELIR
                     LGLAP"
     gene            complement(7129..7686)
                     /locus_tag="P5313_04035"
     CDS             complement(7129..7686)
                     /locus_tag="P5313_04035"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRY79568.1"
                     /translation="MSGSRPRPPKDSTFALAIVLVLVGTALLFLTTGAMPGPARLWPM
                     GVAAAGSVILYLGATRSLASAWFFTGSAFVLSSVLLIVRGFLEWPLSWYWPLFMVVLG
                     LSSLVTGWNRYRRPRAAYLVPAFSFVVLGIFFSLFSFDVIRFSFRRFFTEWWPVFLIA
                     AGLVLLTLYFYNRTRFSRPPERHGR"
     gene            7752..8816
                     /locus_tag="P5313_04040"
     CDS             7752..8816
                     /locus_tag="P5313_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY79569.1"
                     /translation="MVPRTAAAVCAALLVLLAACGAPSPSSGRPDSAASAEDREVREL
                     AELFTLLSDPGIKDEERFAVVQTIADAYLRSRDYGRLVSFLTGVVEKDASDPYAAHYL
                     FTVAYAYLQSGAREVAALYFDRIVKNHPDLILRGESIHKVCLERLLELSPSPVRRIDY
                     RRELIARFPDSIDLGAAWFLLGKEYERIGDWDAALAAYQKYLPHFGSSIPGYPDAFQY
                     ARTLIEFANSPKDWTFESLDDLVGRVQAALAQGSPRTLQRLRAKVGFFAMSWQQDQTE
                     GNSQVLFDFSEFMTGSRIFSAPALDPGSNAREAFLRTWGWSERIATWYLYFRKINFPS
                     DPEIHGRWEWAGIYFGEKLN"
     gene            8813..10264
                     /locus_tag="P5313_04045"
     CDS             8813..10264
                     /locus_tag="P5313_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY79570.1"
                     /translation="MIRGPARRVRVPGILFRLAVFAAAAASAFASDGAPSSTAFPPPE
                     KASPPAAPAARDSPRLLRSVPPGSVPEGPGAPTDPASPGIAEPGYPADPGALTRRPPE
                     DGLSLALPWENERFRKFREAYLSPGGRKWLEAVAARSSPYASYVYERIRWYRLPEELF
                     FLPVIESEWSPRAVSRSGAMGLWQFMRNSIAGYDMRIDDWVDERRDFMKSTDAALRKL
                     RSNYESLGSWEMALAAYNAGLGAVSRAAAAAGTRDYWELSARGLLSAEAVNYVPKFLA
                     AVSVLGRAGRYGLTIPETALETWDRVDLDRPVDLALLSEASGVSLAVLRTGNPELRYD
                     VTPPEGRYALKVPVGAAPAVREVLARPGFQALRYYQHRIRSGDTLSALARHYEVSVPL
                     ILRLNPGVRPEALRIGSTLVIPALKEKGPYAPASPSESLEFSGSYVVTKGDTLWSISL
                     RYGVQPEVLAERNGLDISSIIREGMTLKVPKME"
     gene            10289..11266
                     /locus_tag="P5313_04050"
     CDS             10289..11266
                     /locus_tag="P5313_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79571.1"
                     /translation="MKRSPEHRRPAYRLLPAALWAAAALWAAGLLPASAQAGSSGMSA
                     SPVPAAPGPAATAAAGSALPATSYSTSALLRWDTMTLAARVSLDLRAAGIRLPAGRSE
                     AENMIERDLPGLLQETVFRIPVDSYRTVGDTLADGTLSINDLLNLIRSLSRRESSFSP
                     DFRTFSALYELPLTDIASLYVRHSRPYELAAPLDYTPSRPYSGIVIYAKGELPVHGEH
                     VRARAVPCLFPRIFDERMNTILERNLVSPEAIRRWGLVGYTEDLAAAALEERTGDNPL
                     RIAASAVFGTSRTDLVISREEALRILTLPENRALLREGRVVVVIDKSLP"
     gene            complement(11337..11846)
                     /gene="tpx"
                     /locus_tag="P5313_04055"
     CDS             complement(11337..11846)
                     /gene="tpx"
                     /locus_tag="P5313_04055"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRY79572.1"
                     /translation="MAVIKFKGNPVHTAGDLPAPGTAAPDFRLTKGDLSDVSLSDFKG
                     RVRILNIVPSLDTSVCALSAKRFEQEIKKRPGLVVLTISRDLPFAQGRFCKTEEVENV
                     VMLSELRNRDFGKAYGAEMKDGPLSGLLSRAVVVVDSAGKVVYTEQVPEIAQEPDYEK
                     ALAAASKAN"
     gene            complement(11982..12054)
                     /locus_tag="P5313_04060"
     tRNA            complement(11982..12054)
                     /locus_tag="P5313_04060"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12019..12021),aa:Phe,seq:gaa)
     gene            12243..14543
                     /locus_tag="P5313_04065"
     CDS             12243..14543
                     /locus_tag="P5313_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY79573.1"
                     /translation="MRFLLIQPPFVQLNAPYPAVHCLDRFLRDRGHDTVVRDDSAGLY
                     RELMAPRSVRRILSDAETALKARPAPDPETALQTARYLSYRDRWVEWAEPLARFLSGG
                     DPALAFRLSRVPDDLPLGRRAETFLEEREGLLKPEDAPALAIRILDDLGDLVAYALDP
                     DFGTVRYAERIASSRAEYGPISEAAESGYLMREFYRPRLREEFRALSAPVPDFALLTV
                     PFPGCLAGAVAAAREVRRAFGPGVRIALGGGYVSTELRRLRDASVFSDIDYLCFDAGY
                     GALASLIEGAEAERAGRPVPAPYRTARRAPDGEVEYCGQPPEDDADPAGIRRTPCPDG
                     DRFASLEEEALRSTFPDYRNADFGSCVRAPYSDNPMHRIWSDPPWLKYQLAYGCYWHR
                     CSFCDTRLDYVRRYLPSDPGALFDAARAARDRTGLSGIHFTDEALPLPGLLEFARRNR
                     AEGRPFSFWGNVRFDRVWTADRCALLAASGLTAVSGGIEIATDRGLAMTGKGFTFPDL
                     VRALAAFKRAGVLVHGYLIYGFPAQTPGDLVDSAEAVRQLFAAGLLDSAFWHRFVLTR
                     HSELYARWRTGKEPDLQPVDRGGNFATNDLEFRGSERWDPYDGPLEALLADWMAGKGL
                     DRPVRPLVPGGPGPSLAPDRVGTLARAAAEHPYEGEVLPARPRVHWLAGLPALTAVRA
                     SSGGRAASARTAAGGLARVEWFDRGRPAGIDLPRPGAERLAEALAALARRPDGMDPDE
                     FLAAAGAVGAGDGLLTALRGEGLVLV"
     gene            complement(14540..15766)
                     /locus_tag="P5313_04070"
     CDS             complement(14540..15766)
                     /locus_tag="P5313_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRY79574.1"
                     /translation="MSDKSKIFAHIEASVDGIVELESILTAVPAMAPESGGTGEMKKA
                     EVLLAWLAKKGIRNIERFDAPDDRVPGGLRPNIVATIPGRDDSGRLWIMSHLDVVPEG
                     DRSMWKTDPFTVVRKDGRVYGRGVEDNQQGLASSVFAALALLENGIVPTHTVKLLFVA
                     DEEYGSKFGIQHLLAKHDLFRKDDLIIIPDGGCKDGSEIEIAEKNIAWVKVVTKGKQC
                     HGSTPDEGANAFLAACDLALRVHELEKTVFTARDSLFHPDRTTLSPTLKEANVPNVNT
                     IPGDDVFCFDMRILPQYPIAQVLSEVEKVMRQVEAAHGVKMSYEILNRNESVATKAEA
                     PVVKALSGPVEEVYGVKAKAIGIGGGTVGAYLRNAGFDCVVWARLEETAHQPNENALL
                     SNIVGDAKVFASLMLR"
     gene            complement(15862..19269)
                     /locus_tag="P5313_04075"
     CDS             complement(15862..19269)
                     /locus_tag="P5313_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRY79575.1"
                     /translation="MSGGNRASDRDIRTVFGNRLLLADGSTGTALEELAPGRSATFLP
                     LEAPEIVEELHRAYFDAGSDLVETATFGANERALAKAGAPGAARELNRAACEAAVRAA
                     RRAEEADGRPRWVAGSVGPGEDPPSLGASTYAELVESYRPQMAGLLEGGADLVFIETC
                     QDPLQVKAALGALFDARAEVGREVPFIVSATVDASGRMLTGTGMEALAAIVAPFRPLA
                     LGLNCSGGPAELAAALEALARASPAPLSLMPNAGFPRLEAGRAVYPMGPGDFARETAA
                     LARRFGVSLAGGCCGTGPAHIRALAGLLADRPRPSPRPARKPGLASLYQAWPPESGGV
                     SKAADASPAAFLRVGEKANAAGSAAFAKRVLAGDVEGQAALALAQEESGAHALDLHLS
                     RPGRDEAADLAALTGRLAGTARAALSLDSPDPEVLAAALPLVGGRPLLNSANLEDEAK
                     ARRVFRLARRFGAAVVCLALDGGGPARDTAGKVRICRALHDLALEEGLAPEDLYFDCL
                     TFSLASESGGFEGAAARTLEAIPRVKEACPGSSTILGVGNCSFGLPRPLRPPVTAVFL
                     EAARAAGLDAAILDPGLPDPETVDVGIRSAVRTLIGGEAGVEARAAALDSLLARAPGA
                     AEAAAQDTPGAAPEDRLRAAILRGEAGAAARAAREAAGHPPVTSLVASAMSEVGARFS
                     AGDLPLPRVLRSAEAAQAALGELTPGSEVQGLGRGTVVMATVKGDLHDIGKNLTGLIL
                     GGSGYRVVDLGTDVGIERLLAAVGKEGAAALGLSGLLTRSLEEMRKAARALGDSGSEA
                     LLLLGGAAVDPRFVEAELAPLHPGRVRACADAFEALRVLAKDREPGASDPATGPATRP
                     AAAAPAAEASGAPSDAGPALPGAPAPDGRKRSRIPGLPPTPPWTPPFRGSRPADGLSP
                     EELFAALDRKTLLQVRWGFGRRGREEAEESFERISGLARSRGLCRAAGVYGYFSCEHR
                     GGGLLRVDDGRGGAREFRFPGAGGPGGPTIAAYFDGEGSVIPFFAVTAGPELSRAAAE
                     LRASGDPREYFLLHGLAAALAEAAAEVLHRRIGRELEAAGAGTAGRRYSFGFPGCPGV
                     EFQGELLGLLGAARVGIGVTEGHQLTPEFSVTAFVVPRPDAEYFSPP"
     gene            complement(19266..20294)
                     /locus_tag="P5313_04080"
     CDS             complement(19266..20294)
                     /locus_tag="P5313_04080"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRY79576.1"
                     /translation="MNDTNAAVPLADGIFSIEVVPPARGAGLEDLFAAVETLLPYGPS
                     KVSVTDHPAGPPDGAPLPGGGFARSGPRSKPGTLGTALALQSAFRVRAVPHLVSRGAD
                     RYAMEDLLIDLHYAGFRELFVVRGDDRFHRDPGDAGAGPESPVRGYSSAADLVAHVAA
                     LNRGEYLPPAEPGKPTDFRVGVAGYPEKHFAAPNKEADLRKLKEKVDAGASYIVTQMV
                     FDARRYFEFVAALRGAGIAVPVIPGIKPLTRASQAAAVPRNFFVDLPDSLLRSLEGAR
                     TPEEERSVGIRAAARLAEDLLSGGAPALHFFTMGRGGATRDVLDAVFGGPEGARIRGA
                     AGFGGNIQ"
     gene            complement(20291..21148)
                     /locus_tag="P5313_04085"
     CDS             complement(20291..21148)
                     /locus_tag="P5313_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HRY79577.1"
                     /translation="MYQPRTYRALAAAERFAYFPVRYRETDLWIGVDRPSFRSSLGQD
                     VLERVIDLRLRLESYIERRPEFRSSFEPIPDDPGAPAIASAMLRAAREAGVGPMAAVA
                     GAFADAAARFLLGPGRCRELVVENGGDIALSGESEFTAALLAGTSSLSGALGLRIRGG
                     GYRGLAASAGRVGPSYSAGRADACAVLARDAARADALATAFGNRVGSGADIEGVLDLA
                     RRTDGVLGIALIAGSTFGCAGDLELVPLAAGRGRACKSPKGGPDSETRRRSDPAAPET
                     RTVRAGRLP"
     gene            complement(21135..22601)
                     /locus_tag="P5313_04090"
     CDS             complement(21135..22601)
                     /locus_tag="P5313_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HRY79578.1"
                     /translation="MRTKTYEEINEKLARGDAVVLTAMEFKDLARDLDPAELVREVDV
                     VTTATFAPMCSSGVFLNFGHSDPPIRMEEISLNGVPAYEGLAAVDAYLGATAEDPEDP
                     GYGGAHVIEALVRGEEVRLSARGKGTDCYPRTSMETSVSLDRLNEAWFFNPRNGYQNY
                     GAAVNGSGRPLRTYMGLLEPDYGSVSYSTSGELSPLLNDPECRTVGTGTPVFLCGARG
                     RVTGPGTQYCDRREAGRSGLPVAGARTLALWADLKEAEPEFLAAARYDGYGISLFVGL
                     GLPVPVLDEDLARRLSVRDGDIETEVRDYSRPGRPVTGRCTYAELRSGSVELAGRRVR
                     TSSQSSLAKARRIAEVLRERVLEGRFPLEAPREPLPEGRTPRGLAERAFVSIPGSAES
                     TAGDPGVGGAGYRRDLCVDCGSCAAHCPRGALAIGAPEWRLRYVTGLCDGCGACFGAC
                     PRKALGAGKAAAAAGSISGRTAEGRAGSSNKRIIRVPA"
     gene            complement(23130..23966)
                     /locus_tag="P5313_04095"
     CDS             complement(23130..23966)
                     /locus_tag="P5313_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRY79579.1"
                     /translation="MPELPDLVYIVERLRPALLGRRIVDASVGNPTVLRILVPGDFRE
                     LLAGDTFRTLERRGPFLRFSLDRHDLVMHLMLAGRVRVVPAGEKPLAARRFALDLEDG
                     TRFEYGDEKNMGKVYLCEPGRFEAIPGFLTQGVDLTGPDFDREVFHRLIAKERRQARV
                     FLMDQTKLSAVGNAYADEILFSARIHPKAPCSRLSPHEKDAFYTAIREVLAWGISEVA
                     EAGKPTEEKVRDHMRVRNRAGSPCPVCGTTIRKAGVLGFDSFFCPGCQKPKTEQFIDW
                     SR"
     gene            complement(24094..24846)
                     /locus_tag="P5313_04100"
     CDS             complement(24094..24846)
                     /locus_tag="P5313_04100"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013553824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRY79580.1"
                     /translation="MSPEASRAVLRAAQKLGQAKRLIAFTGAGISVESGIPSFRGEGG
                     IWTRYDPATLELGRFLRDPAAAWPTIREIFYEYTSKAEPNDAHRILARWEAEGRLAFL
                     VTQNIDGLHEAAGSRALAEFHGSCRHLVCLDCGTRVEAEPALLADLPPRCRCGGIFKP
                     GFVFFGEGIPRNAYEASFAAAETADACLVIGSTGEVYPAAEIPRIVKRRGGLVVEIDP
                     EPTGFTESVTDIFIRAGASEAMRELDRELVNG"
     gene            complement(24919..25200)
                     /locus_tag="P5313_04105"
     CDS             complement(24919..25200)
                     /locus_tag="P5313_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79581.1"
                     /translation="MDTRNGLVTFGLFVLLFAFTFVFSMVALSEDNVAYGILALIGFL
                     VCIGASVFNSVLAAQEGAIFAVWFRTYAILVGILFVWFLTRVGTAFGWW"
     gene            25349..25891
                     /locus_tag="P5313_04110"
     CDS             25349..25891
                     /locus_tag="P5313_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRY79582.1"
                     /translation="MKRAVVFMANGTEEVEAITPIDYLRRAGVEVDVAGIGERDLMGA
                     HDIRLTADLAADDLEGPFDLVVVPGGRGCEAIGKDESARNLIVKHFREGRLTASICAA
                     PVWVLGKHCGILAGRRFTCYPGLEKDPYGGTFVAERVVEDGNLITSRAAGCAGDFSVA
                     VIRALLGKAAAEELASKVLL"
     gene            complement(25955..26368)
                     /locus_tag="P5313_04115"
     CDS             complement(25955..26368)
                     /locus_tag="P5313_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRY79583.1"
                     /translation="MKEQGTTTRIDGSVVTLRIDLEANCAGCMNKEGCGLAGSLIKAQ
                     DPGGLVSAPGQRVEVEISSGAQAAGAFWLLGLPLVLFGAGYAAGAALFPASGEGPAAL
                     LGIGGFALGILAAFLLKRGTRAASLPVVTRVLRLP"
     gene            complement(26337..27164)
                     /locus_tag="P5313_04120"
     CDS             complement(26337..27164)
                     /locus_tag="P5313_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRY79584.1"
                     /translation="MSVRILYIAEIVGKTGVFVVKNLLPGLRRDLKPDLVVACADGAT
                     GGAGVGRAHATYLRKLGADILTTGERGFYKKDIVELFPKASWLLRPANYPYGVPGHGW
                     KVYAAGERKIAVVQLLGQSGFPRTHLANPFLLMDDLLQRLKQETPVVIVEFHAATTAE
                     KITLAAHLDGRVTAVIGAHTRVPTADARVSPKGTAAITDAGRTGAFLSVGGMDPAGRV
                     REYLSGIPEWTRDGGGNLELQGCLVESDENGRALSLELLRVPCKEVMVERTGNDDED"
     gene            complement(27227..27712)
                     /locus_tag="P5313_04125"
     CDS             complement(27227..27712)
                     /locus_tag="P5313_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HRY79585.1"
                     /translation="MEEYRRPSWDEYFMEVTNAIAKRATCDRGRSGCVIARDNQVLAT
                     GYVGAPAGLPHCDEAGHQLKQTVHEDGTATTHCVRSVHAEQNAICQAAKNGVPIRGAT
                     LYCRMTPCRTCAMMIINCGILRVVCERKYHAGSESEAMFRTAGVKLEYVHEEVEKYEK
                     Q"
BASE COUNT         4264 a         9862 c         9790 g         4035 t
ORIGIN      
        1 agcccttgct ccagatgagg cggccgtcct tcacgatgcc cacggccagg ccgggctggt
       61 cgaagtcggt gacggccttg gcgaggtacg cgtcgagcgc gttgaggtcg ggacccggtt
      121 gggcgtgggc cgcaggaagg ctgaggagcc cggaaaggag gaggaagtga cgcaggttca
      181 tggcgcgggt tgaaggtgcg ccaaggtaac caagcggtgc ggccacgaag ctgcggcccc
      241 gtgctcagcg gttgggtggt ggaccgcccg ggccgccacc gccgcgtcgt gggtcaccag
      301 gaggaccgcg gtcccggccg cgttcacctc cgagaggatg tccaggatct cccgtgccga
      361 ggcggagttg agggccccgg tcggctcatc cccgaagagg accgcgggat cccgcaggag
      421 ggcccggcag atccccgccc gctggagctg cccccccgag acctgggaga ccgagcggcg
      481 ttccagcccc tcgatcccgg tcctacgcat gaggtcccgg gccttttcga cgtggcggcc
      541 caggccccgc cgctcgtccc gcaccgcggg caggatgatg ttgtccagga ggtcgaggtt
      601 ccccagcagt gtgggctgct ggaagacgaa gcccatgcgc cggcggcgca ggtccgcgag
      661 gtcgtcgtcc cgcagtcccg agaggtcccg cccctcgaag aggatccggc cgccgtcgac
      721 ccggtccatg ccgctcaggg catacaggag tgtggacttc ccggatcccg agggacccat
      781 gatggagacg aactcgccct tccggatctc cacggagacc ccgtccagga cgttccggcg
      841 ctcggctccg tcgccgaagg acttcacgat gtgttcgccg ctcaggatgg tgttcatggt
      901 tactcccgga tggtctgggc tatcccgatc cggccggctc ccgcagcccc ggccgccgtg
      961 gccgcgaggg ccgcgagcgc gacggctagg gggatcaaga tgtaggccga aagggggtcg
     1021 gcggcgaact cgaaggaggc ggcgccgaac agggatatca gggccccgac gagggactcg
     1081 ccgagggtgt tcgcgaggag ggaaccggcc aggaccccga tcccggccac gagggcggac
     1141 cgggccaggt actgccgggt caggtccgag ttccggaagc ccagggcccg cagggccgcg
     1201 atggaaaccc ggtcccgggc cacgagcatc cggacgaaga gcgccgtgat cagggctgcc
     1261 aggaccaggg ccaccccgag actgatgcgg gaggcggatc cgaccgcccg gatcgtgggc
     1321 ccgaaggtct gggccacgta ctcctggagg ctggagacct tggcgtaggg gaaggcggcg
     1381 gcgtaggccg cggccttttc ccgggatcgg gacggatcgg ccagaccggc ctggactacg
     1441 gcccacagga ccggggcctc cccgcccgag aaggcggcct tcgcggtctt cccgccgttc
     1501 gtcacgtccg agtagaggcc ggccacccgc agatccctcc actccccgtc caccgccgcc
     1561 gcgagccggt cccccacccg cgttccgaga tcctcggcgc tcaggacgga gagggcgatc
     1621 tcgtccggtg ccccgggcgc ccttccctcg gcataggcca cggggaagac cgcgtggtcc
     1681 ccgagctcga ccttgaggct cgactcggag ccgtccttcc cgcggaccgt gaaggcccgg
     1741 gccgtgagga cgacggtctt gcgaacctcg ggatccgcgg ccagggcgga cgcgatctcc
     1801 gaggcccgtc cgggtatgtc cccgaccgcc tgtacgtccg cccggaggtc gcaggccccg
     1861 atgcccaggt aggagctgaa gctcggggag gcgacggtgg tgtggaggtt cctgggcact
     1921 acggcgatga aggaggaggc gaccaggacc gcgagcatcg tcccgtagag cggtatccgg
     1981 gccaggacgt ccaggaagcc gagggccgcg ttggccccca ggggcagccc cgcggccaga
     2041 cggaggcggc ccggacggcg ggcggcctgc cggggggcgc cgaagcggag ggcctcggcc
     2101 gcggagatct tccggaagcg ccccaggact ccccggacat acccgaggac caggaggccc
     2161 accaggaggg ccccggcgag tcccagggcc ggggccaggc gcgaggcccc gccctctccc
     2221 atgaagagcc ggaggttggc caggaggggt ccccggaagg cgaaggagag ggcccagcca
     2281 aaaagggctc ccgggacggc caggaccgcg tacttggcca ggtaggcccg ccggatgtcc
     2341 gatacccgga gtccgacggc cttcatcgcc ccgatctccc ggtagtcctg ctcgatcttg
     2401 gccaggaggg cgaagcggac gcagagcagg gacacgaagg taacgaggag acccacgaag
     2461 aggaggacga cgatcagcag gccgtcggaa agggcgttca tcatccggaa gagggggaag
     2521 cccacgcggg gaccgttgga ctccagcccc gcggcgacat aggccgcctc gaggttcccc
     2581 agggccccgg ggtcgcggag gcggaactcg atgaggtgct cgacggagcc ccggtcccgg
     2641 agggtggcgt aatcctcagg gctcaccagg aatcgcttgg aagaggagag ggcggagttc
     2701 atctgggagt cccggacgaa gccggccacg cggaactccc ggcccaggat ccggacccgg
     2761 tccccggcgc gggcggtgcc gtccttgaaa tagaacacgg ggacgtagag ctcgccctcc
     2821 cggggattcg cgacgttccc gtccaggtcc aggaggaagt cgaagtcccg gccctggatc
     2881 gaaagcccgt tgtcctggac gctgccggcc agggtgcggt ccccgaactc gaaggcggct
     2941 ccctcgacgt tcaggaactc gaggacctgg aagccctcca actccgaact ccctgcggcg
     3001 aaggattcaa ggcgctccag gtcgatcggt cccgagtgca tctgcaggag gtggggcgtc
     3061 gcggcccgtt ccatcagcgt ctcgagggcc gcggagagct ccgcccccag ggcccccgcc
     3121 agggagacca ggagggcgga gaccatgacg aagagcaccg tcgccaggga ggccagccgg
     3181 ctcctccgta tgtcgttccg tatgatccgt gcgtacatgt ctaccgtccg tccttcggat
     3241 tctttccggg gcggccccgg gagccgcgcg ttccgttctc aggttcaccg ccgaatgccg
     3301 accgcaccct cgagaggtcg tccgtgccga gcccgaacaa acggccgatg ttgaagacca
     3361 gggcccggag ccttccggag cgctccgcct cggagaggtc cacgagtccc ccgtcgatga
     3421 cctcggtcat gtagacgacg accagttcca tgctctcctc gggataggga gtgtcgaagg
     3481 ctcccgccga gatcccctcc cggacgatgc cggacaggat cgaggtcatc ccggccacga
     3541 ggcggcgctg gacgcggtcg tggagaggaa ggtttccggg atcgtggagg tgctccagga
     3601 ggccctgccc cacgtcgccg gaggcctgga aggccccgat cacggccagg agccgctcca
     3661 gggccggccg gtccctcaga gcctcccccg tctcccgggc cgccttgaag aggcggtcgc
     3721 agagccggtc gaccagggcc tccatgaccg ccgccttcga ttcgaagtgg tagtacagag
     3781 ttccccgggc tatgcccgtt cggtccagga tgtccccggt gctcgtcccg gagtacccct
     3841 gctcccggaa cagcgcttcc gccgcgtcga ggatctcctg ccgccgttct tccgcttcca
     3901 tcatcactct cacagtacta gtagaccgac tgtcggtcgg tttcgacata ataccgttcc
     3961 cggaattccg tgtctagaag ccgggagcct acaaaccgac gaattcaatc gggcaacgcg
     4021 gactcgccct tcagcctcgt acatccttgt ccgaggcttc cgggccgggg ttctcgctct
     4081 cgaggccgtc catggcctct cgcacgggcg atcctcgatg tctcggatcg cccgtgccgc
     4141 tcgaaccctt cgagtccgcg ttgcccgaga aggcttgtca gagggcggcc aataaataaa
     4201 aagcggtccc cgtttccgga aaccgcttct cgggcaacgc ggactcgaac cgcggaccca
     4261 ttgggtcttc atgcagtttt cggcgaagcc gaaaactgcc aggtcaacaa ggcgctagcc
     4321 ttgttgaccg gtccccagac tcatcgtcac gatagtgatg gaaaaaaatc ggtcgggcaa
     4381 cgcggactcg aaccgcggac cccgagtccc ccagactcgt acgctaacca cctgcgctat
     4441 tgcccgaccg atttttctct attggtcgtt gtgacgtcgc taaccacctg cgctattgcc
     4501 cgtcgcgccc ttgggcgccc gaaaaatcta ccacgctccc tccccgaggg tcaaggccgg
     4561 gggcgcggct cgggatcgtc gaaacagcgg aaataggcat ccgcttccag acaggggtgg
     4621 attcccggac gggtcagggc gaagtcgtac cgcacaggat cgtccggccg aaggcggcgg
     4681 aaggcctcgg tggcttccaa ggcggctttc aggtcgtcgg ctctgcgggt taccaagccc
     4741 aggcggcggc atgccgcccc catgtgggta tccaggggaa cgaccaaccg ggaagggctc
     4801 accccggtcc agccgccggg gtccacggcg tccttccgga ccatccatcg caggtatagg
     4861 aagaggcgct tgcaggcgga gccccggctc ggccgaggca ggaggttgtc cctccaggga
     4921 gcttttcccg aaacccgtat ccaacgggag cccgcgaaat cttccagggc ggcggcgaac
     4981 cggtccgccc cccggatgat cgtcggctcc gagggatcgt ccccggcccg gaacaggacc
     5041 tccagggacc cgtgccgggc ccgggcgttc cggacggcgg acaggagggc cgccaggtcc
     5101 tcccccccga agaagcggta ccggaacccg gaaaggaggc gggcgaggtc gtcgtccccc
     5161 agggctccca gggcgtcggc cggggaggag ccgaggggcg ccaggacttc ccgggccgcc
     5221 cgcacgatga ggggggcgga gcccagggcc aggcaggcgg cggccaggcc cgccacctcc
     5281 cgatccccgg ggtcgacgta ctccctcgtc acggccagag gatccgggtc gatcagctcc
     5341 ggccgggcca gctcggcgta ggcggtttcc aggaaggcgg aaaggtccgg agcgcggctc
     5401 cggggggtcg gcgggttcag ggggccaggc ccaggcggat gagctcctcc cggcggcgag
     5461 ccagggcggg gtcgtcgggg gccacggcca gggcctggcg caggtagaac cgggccttct
     5521 gggcgtcctt ctgacgcatg tagatgtcgt acatggcggt cagggcctcc aggttctgga
     5581 cgttctccac caggctggag cgcagggaat tcagggccgc ctcgtcgttg ggctggacga
     5641 ggctctggag gaagtagagg ctcgagcgga accgggcgga ccccttttcg gtcagggctc
     5701 gggcgatgag atcccgggct ccgcctgagt ccctgcggtc gataagggag cggatgtagg
     5761 cgatccgggc gtcctcggag tccggggagg cggtccgcca ctccgaggcg acccggaagg
     5821 cttcctcgaa ccggcccgcg gaccggtaga ccaggtacag ggaggcccgg tcccggtact
     5881 cgggatccgc ggcccggagc cgctcctggg tggccagggc cgaggtccag tccctgcggc
     5941 ggatctcctc ggccaggagg atccgcaggg cgccgggatc cgcgggatac ttagccagga
     6001 cgcggccggc catcaggtag gcctcccccc gctccgagga ggtgcccagg gccaggatgc
     6061 gggccgcggc caggagggtc tcggagtcgt cgggatagat cgagaggagc ctgcggaaga
     6121 accgcagggc ctcgtccttg ttccgggacc cctcccaggc gacccttccc cggaggaaga
     6181 gatagcgccg gtccgcgggg tccacggccg cgtaggcgtc caggaggggc gctgcctggc
     6241 ggtagtcccc ggcccggacc agcatgtccg cccggataag gaggatccgg gagtccaggg
     6301 gatcctccag gagcaccctc atgacgaagg gtcccgcctc gtcgtagcgc cccgagtcgt
     6361 acagggccag ggccatgagg cggacccagc cgaagctgtc cccgaaggcg gttttggagg
     6421 ccgatagaat gtccaccgcc tcctggggcc gtcccagggc gatcagggtg cgggccgctc
     6481 cgaagagggc cggatagcag tccggggcgg cgacgaaggc cgcgacgtac cgctcctgcg
     6541 cggaggacag gttggcggac cgctccgaga tcacgccctg gaggtggtcc gccaggggcg
     6601 actccaggcg ggtctgggcg aagagctcca gggccagggt cgccgcccgg gtggtctcct
     6661 tggaatcccc ccggaagagg gccgaggcca gggccagaag ttccagggga tcggcccctt
     6721 cccggggagt cccgatccgg ccggtccggg cgtccgcgaa ggcccgggcc caggggtggc
     6781 ccgagggcgg ctccggcgcc accggaacgg ttcccagccg ttcgggatag gccatacgtc
     6841 ccagctccac ggcgaaccac cggtacagaa gcacgtccga gggactcagg gccctcgagg
     6901 attccgccag ctcacgggcc cgggacaggg cgtccggggt tccccgggcg agcagggtct
     6961 ccacggcggt cttggccgcc gcggactccc cggacctccc ctgcatggtc agggccgtgc
     7021 cggtatcggg ggggggaggc gtggagacgc aggcggtcag gaccaggacc gccccgagga
     7081 cggccgcggc gagcgcagcg gctgcggcct tcggaccccg ccggtacctc atcggccgtg
     7141 cctctccggg ggtcgggaga accgggtccg gttgtagaag tagagggtca acaggaccaa
     7201 gcccgcggcg atgaggaaca cgggccacca ttccgtgaag aaccgacgga aggagaaccg
     7261 gatgacgtcg aaggagaaga gggagaagaa gatccccagg accacgaagg agaaggccgg
     7321 caccaggtag gccgcccggg gtctgcggta ccggttccag cccgtgacca ggctggacaa
     7381 gcccagcacg accatgaaca ggggccaata ccaggaaagc ggccattcca ggaagccccg
     7441 gacgatcagg aggaccgagg agaggacgaa ggccgagccc gtgaagaacc aggccgaggc
     7501 cagggagcgg gtggcgccca ggtataggat gacgctgccc gcggcggcca cccccatggg
     7561 ccagagccgg gcgggtcccg gcatggcgcc ggtggtcagg aaaagcaggg ccgtgccgac
     7621 caggaccagg acgatggcca gtgcgaaggt ggagtctttc gggggtcggg gccgggagcc
     7681 gctcatgtat aggagtatag ccgcgcggcc cgttcttgcc aacgccgaga cggtcatgct
     7741 aggctttttt catggtcccc cgcaccgccg cggccgtctg cgcggccctc ctcgtcctcc
     7801 tggccgcctg cggcgcgccc agcccctcct ccggccggcc cgattccgcg gcttccgcgg
     7861 aagaccggga ggtccgggag ctcgccgagc tcttcaccct actctcggat cccgggatca
     7921 aggacgagga gcgcttcgcg gtcgtccaga cgatcgcgga cgcctacctc cgctcccggg
     7981 actacggccg cctggtgtcc ttcctgaccg gggtcgtgga aaaggacgcc tcggacccct
     8041 acgcggccca ctaccttttc accgtggcct acgcctacct ccagtccggg gcgcgggagg
     8101 tggccgccct ctacttcgac cggatcgtca agaaccaccc ggacctcatc ctgaggggcg
     8161 agtcgatcca caaggtctgt ctggagcgcc tcctggagct ctccccctcc ccggtgcggc
     8221 gcatcgacta ccgccgggag ctcatcgccc ggttcccgga ctccatcgac ctcggggcgg
     8281 cctggttcct cctgggcaag gagtacgagc ggatcgggga ctgggacgcg gccctcgcgg
     8341 cctaccagaa gtacctgccc cacttcgggt cctccatccc cggctacccg gacgccttcc
     8401 agtacgcccg taccctgatc gagttcgcca actctcccaa ggactggacc ttcgagagcc
     8461 tggacgatct ggtggggcgg gtccaggccg ccctggccca ggggagcccc cggaccctcc
     8521 agcggctccg ggccaaggtc gggttcttcg ccatgtcctg gcagcaggac cagaccgagg
     8581 ggaacagcca ggtcctcttc gacttctcgg agttcatgac gggcagccgg atcttctccg
     8641 cccccgccct ggacccggga tccaacgccc gggaggcctt cctccggacc tggggctggt
     8701 ccgagcgcat cgccacctgg tacctctatt tccggaagat caacttcccc tcggaccccg
     8761 agatccacgg ccgctgggag tgggcgggga tctacttcgg ggaaaaactg aattgatccg
     8821 tggcccggcc cgccgggtcc gcgtcccggg gatcctcttc cggctggccg ttttcgcggc
     8881 ggccgccgcc tcggccttcg cctcggacgg ggccccctcg agcacggcct tcccgccccc
     8941 ggagaaagcc tctcctcccg ccgccccggc cgcccgggac tcgccccgcc tcctccgctc
     9001 cgtcccgccc gggtcggtcc cggagggacc cggcgcgccc acggatcccg cttctccggg
     9061 catcgccgag cccgggtacc cggcggatcc gggagccctg acccgccgcc cccccgagga
     9121 cggcctgtcc ctggccctgc cctgggagaa cgagaggttc cggaagttcc gggaagccta
     9181 tctttccccc ggggggcgca agtggctgga ggccgtggcc gcccggtcct cgccctacgc
     9241 ctcctacgtg tacgagcgca tccgctggta ccgcctgccg gaggaactct tcttcctgcc
     9301 cgtgatcgag tcggagtggt ccccccgggc ggtgtcccgc tccggggcca tggggctctg
     9361 gcagttcatg cgcaactcca tcgcgggcta cgacatgcgc atcgacgact gggtggacga
     9421 gcggcgggac ttcatgaaga gcaccgacgc cgcgctccgc aagctccgga gcaactacga
     9481 gtccctgggc tcctgggaga tggccctggc cgcgtacaac gcgggcctgg gtgccgtgag
     9541 ccgggccgcc gccgcggcgg gcacccggga ctactgggag ctctccgccc ggggactgct
     9601 gtcggcggag gccgtcaact acgtgcccaa gttcctggcc gccgtgtccg tcctgggccg
     9661 ggccggccga tacgggctca ctattccgga aaccgccctc gaaacctggg accgggtgga
     9721 cctggaccgg cccgtggacc tggcattgct gtccgaggcc tccggggttt ccctggcggt
     9781 cctgcggacc gggaaccccg agctccggta cgacgtgacc cccccggagg gccggtacgc
     9841 cctcaaggtt cccgtcgggg ccgcccccgc cgtccgggag gtcctggccc ggccgggctt
     9901 ccaggccctg cgctactacc agcaccggat ccggtccggg gacaccctct ccgccctggc
     9961 ccgccactac gaggtctcgg tgcccctcat cctccggctc aaccccggcg tccgtcccga
    10021 ggccctgcgc ataggctcca ccctggtcat cccggccctc aaggaaaagg gcccctacgc
    10081 ccccgcctcc ccctcggagt ccctggagtt ctcgggctcc tacgtcgtta ccaagggaga
    10141 caccctgtgg tccatctccc tacggtacgg ggtccagccg gaggtcctgg cggagcgcaa
    10201 cggcctggac atctcgtcca ttatccgcga aggaatgacc ctgaaggtgc cgaaaatgga
    10261 gtgaggcgga acgcctcacc gaccacccat gaaacgttca cccgagcatc gaagaccggc
    10321 gtaccgcctt ctcccggcgg cgctgtgggc cgcggcggcg ctctgggccg ccggcctcct
    10381 ccccgcgtct gcccaggcgg gctcttcggg catgtccgcc tccccggtac ccgctgctcc
    10441 gggtccggcc gcgacggccg ccgcgggatc cgccctcccc gcgacatcct actcgacctc
    10501 cgcgctcctc cgctgggaca ccatgaccct ggccgcccgg gtatccctgg acctccgcgc
    10561 ggcgggaata cgccttcccg cgggccggtc cgaagcggag aacatgatcg agcgggacct
    10621 gcccggcctc ctgcaggaga cggtattccg gattcccgtg gattcctacc ggaccgtggg
    10681 ggataccctg gcggacggga ccctttccat caacgacctg ctcaacctga tccggtccct
    10741 gtcccgcagg gagagctcct tctccccgga cttccggacc ttctccgccc tctacgaact
    10801 gcccctgacc gacatcgcgt ccctctacgt gagacacagc cgcccctatg agctggccgc
    10861 ccccctggat tacacaccgt cccgtccgta ctcggggatc gtgatctacg ccaaggggga
    10921 gcttccggtg cacggggagc acgtccgggc gcgggcggtg ccctgcctct ttccgaggat
    10981 cttcgacgag aggatgaaca ctatcctgga acggaacttg gtttcccccg aagccatccg
    11041 ccgatggggc ctcgtgggct ataccgagga tctggccgcg gcggccctgg aggagcgcac
    11101 cggggacaac cccctgcgga tcgcggcctc cgcggtcttc ggcacgtccc ggacggacct
    11161 ggtgatctcc cgggaggagg ccctgcgcat cctgacccta ccggagaacc gcgccctcct
    11221 ccgggaaggc cgtgtcgtgg tcgtgatcga caagagcctg ccgtagacgg aatcgggccc
    11281 gaagcagatc cggcggctcg gccgctcgcc cgccccgggc ccgttcgatc gattccctaa
    11341 ttcgccttcg acgccgctgc cagggccttc tcgtagtccg gctcctgggc gatctccggc
    11401 acctgctccg tgtagaccac cttcccggcg gaatccacga ccaccacggc ccgggagagc
    11461 aggcccgaaa gggggccgtc cttcatctcc gccccgtagg ccttgccgaa gtcccggttc
    11521 cggagctccg acagcatgac gacgttctcc acctcctcgg tcttgcagaa ccgaccctgg
    11581 gcgaagggca ggtcccggct gatcgtcagg accacgaggc cggggcgttt cttgatctcc
    11641 tgttcgaagc gcttggccga gagggcgcag accgaggtgt ccaggctggg cacgatgttg
    11701 aggatccgga ccctgccctt gaagtccgac agggatacgt cggacagatc ccccttggtc
    11761 aggcggaaat ccggggcggc ggtgcccggg gcgggcaggt cgccggcggt atgcacgggg
    11821 tttcccttga acttgatgac ggccatggtt cctcctggag acccggggtg acgcttcgaa
    11881 gcttgtacgg attcgctccc gcgggacggg gtaaaaagta cacaggggga gggttccggg
    11941 gcaaggaaaa caaagggccg cccctttcgg gacggccctt ctgccaccgg tcagaattga
    12001 actgacgaca cgtggatttt cagtccactg ctctaccaac tgagctacag cggcatgtcg
    12061 cggggagttt ataccgtgaa agcggtaggg aagtcaagtc agatggcagg actcgggagt
    12121 caggaaccag gcgacgggcg gccgattatc attcagactg cggatggacg gcaggacgca
    12181 accgccatct agatagtctc tgagtcctga ctcctgactc ctgtctccag tatacttccc
    12241 ccatgcgctt cctcctcatc cagccgccct tcgtccagct gaacgccccc taccccgcgg
    12301 tccactgcct ggaccgtttc ctccgggacc ggggccacga taccgtcgtg cgggacgact
    12361 cggccggtct ctaccgggag ctgatggcgc cccggtccgt acggcgcatc ctttcggacg
    12421 cggaaaccgc cctgaaagcc cggccggccc cggatccgga gacggccctt cagaccgccc
    12481 ggtacctctc ctaccgggac cgctgggtcg agtgggcgga acccctggcc cgcttcctct
    12541 ccgggggcga ccccgccctg gccttccgcc tgtcccgtgt gcccgacgac ctgcccctgg
    12601 gccggcgggc ggagaccttc ctggaggagc gggagggtct tctgaagccc gaggatgccc
    12661 cggccctggc gatccggatc ctggacgacc tgggggacct ggtggcctac gccctggatc
    12721 cggacttcgg gacggtccgt tacgccgagc ggatcgcctc atcccgggcg gagtacggcc
    12781 caatctccga ggcggcggaa tcgggctacc tgatgcggga attctaccgg ccccggctgc
    12841 gggaggagtt ccgcgccctg tcggccccgg tcccggactt cgccctgctg accgtgccct
    12901 tcccgggctg tctggccggg gcggtcgccg ccgcccggga ggtccggcgg gccttcggac
    12961 ccggggtccg gatcgccctg ggcgggggtt atgtatccac ggagctccgg cgcctccggg
    13021 acgcctcggt cttctcggac atcgactacc tgtgcttcga cgcgggctac ggggccctgg
    13081 cctccctgat cgagggggcc gaggcggagc gcgccggtcg gccggtaccc gccccgtacc
    13141 ggacggcccg ccgggctccg gacggggagg tggagtactg cggccagccc ccggaggacg
    13201 acgcggaccc ggccgggatc cggcggacgc cctgccccga cggggaccgc ttcgcgtccc
    13261 tggaggagga ggcccttcgc tcgaccttcc cggactaccg gaacgccgac ttcggctcct
    13321 gcgtccgggc cccgtactcg gacaatccca tgcaccggat ctggtcggac cctccctggc
    13381 tcaagtacca gctggcctac ggctgctact ggcaccgctg ctccttctgc gacacccggc
    13441 tggactacgt ccggcgttac ctgccctcgg atcccggggc cctcttcgac gcggcccggg
    13501 cggcccggga ccggaccggc ctttcgggga tccatttcac cgacgaggcc ctgcccctcc
    13561 cgggcctcct ggagttcgcc cggcggaacc gcgcggaagg ccgacccttc tccttctggg
    13621 ggaacgtccg cttcgaccgg gtctggaccg cggatcgttg cgccctcctg gccgcctcgg
    13681 ggctcacggc cgtatccggg gggatcgaga tcgccacgga ccggggcctc gcgatgaccg
    13741 gcaagggctt cactttcccg gacctggtac gggccctggc cgccttcaag cgggcggggg
    13801 tcctggtcca cggctacctg atctacggct tccccgccca gacccccggg gacctggtcg
    13861 attccgcgga ggcggtccgc cagctcttcg cggcgggcct tttggattcc gcgttctggc
    13921 accgcttcgt gctcacccgg cactcggagc tgtacgcccg gtggcggacg ggtaaggagc
    13981 cggacctgca gcccgtggac cggggcggca acttcgctac caacgacctg gaattccggg
    14041 gttccgaacg ctgggatccc tacgacggcc ccctggaggc cctgctcgcg gactggatgg
    14101 ccgggaaggg gctggatcgt ccggtacgcc ccctggtgcc cggcggcccc gggccatccc
    14161 tggccccgga ccgtgtcggg accctggccc gggccgccgc ggaacacccc tacgaggggg
    14221 aggtccttcc ggcccggccc cgggtccact ggctggcggg actcccggcc ttgaccgcgg
    14281 tgcgggcatc ctccggcggg cgagccgcct cggcccggac cgccgcgggc ggcctcgctc
    14341 gggtcgagtg gttcgaccgg ggccggcccg cgggcatcga cctgccccga cccggggcgg
    14401 agcgcctggc ggaggcgctc gcggccctcg cgcggcgccc cgacggcatg gatccggacg
    14461 agttcctggc cgcggcgggc gcggtcggcg cgggggacgg cctgctgacc gccctccgcg
    14521 gcgagggatt agtactggtc tagcggagca tgaggctggc gaagaccttg gcgtccccga
    14581 cgatgttgga aagcagggcg ttctcgttgg gctggtgggc ggtctcctcg agccgggccc
    14641 agaccacgca gtcgaagccc gcgttccgca ggtaggcccc cacggtgccc ccgccgatcc
    14701 cgatggcctt ggccttgacc ccgtagacct cctcgaccgg cccggacagg gccttcacca
    14761 ccggggcctc ggccttggtg gccacggatt cgttccggtt caggatctcg tagctcatct
    14821 tcaccccgtg ggcggcctcc acctggcgca tgaccttctc gacctccgaa aggacctggg
    14881 cgatcgggta ctggggcagg atgcgcatgt cgaagcagaa gacgtcgtcc ccggggatgg
    14941 tgttgacgtt ggggacgttg gcctccttca gggtcgggga gagggtcgtg cggtccgggt
    15001 ggaagaggga gtcccgggcg gtgaatacgg tcttctcgag ttcgtggacc cgcagggcaa
    15061 ggtcgcaggc ggccaggaag gcgttggccc cctcgtccgg ggtggatccg tggcactgct
    15121 ttcccttggt gacgaccttg acccaggcga tgttcttctc ggcgatctcg atctccgagc
    15181 cgtccttgca gcctccgtcg gggatgatga tgaggtcgtc cttgcggaac aggtcgtgct
    15241 tggccaggag gtgctggatg ccgaacttgg atccgtactc ctcgtccgcg acgaagagga
    15301 gcttgaccgt gtgggtaggg acgatcccgt tctccaggag ggccagggcc gcgaagaccg
    15361 aggaggccag gccctgctga ttgtcctcca ccccccggcc gtagacccgg ccgtccttcc
    15421 tgaccaccgt gaaggggtct gtcttccaca tggagcggtc cccctcgggc accacgtcca
    15481 ggtggctcat gatccacagg cggccggagt cgtcccggcc ggggatggtg gcgacgatgt
    15541 tcggccgcag gcctccgggc acccggtcgt ccggggcgtc gaagcgctcg atgttccgga
    15601 tgcctttctt ggcgagccag gccaggagga cctcggcctt cttcatctcc ccggtgcccc
    15661 cggactccgg agccatggcg gggacggcgg tcaggatgga ttcgagttcg acgatcccgt
    15721 cgacggacgc ttcgatatgg gcgaagatct tggacttgtc ggacatggga gctccttttc
    15781 ggccccggcg ccgcgaaaac cgccgcagga ccggtcttgg tatcggggga cccccatact
    15841 acaacggcga gggcggggag tctacggcgg ggagaagtac tccgcgtccg gccggggcac
    15901 cacgaaggcg gttacggaga actccggggt cagctggtgc ccctccgtaa ccccgatccc
    15961 gacccgggcc gcccccagga gacccaggag ctccccctgg aactccaccc ccgggcagcc
    16021 gggaaagccg aaggagtagc gcctccccgc cgtaccggcc ccggccgcct cgagctcccg
    16081 gccgatccgc cggtggagga cctccgcggc ggcctccgcc agggctgcgg caaggccgtg
    16141 caggaggaag tactcccgcg ggtcccccga agccctcagt tccgcggcgg cccgggaaag
    16201 ctccggcccc gcggtcaccg cgaagaaggg gatgacggaa ccctccccgt cgaagtacgc
    16261 ggcgatcgta gggccccccg ggccgcccgc tccggggaac cggaactccc gggcgcctcc
    16321 ccggccgtcg tccacccgga gaagcccgcc cccccggtgc tcgcaggaaa agtagccgta
    16381 taccccggcg gcccggcaaa gcccccggga ccgggcgagg ccggagatcc gctcgaagga
    16441 ctcctccgcc tcctcccttc cgcgccggcc gaagccccag cggacctgga gcagggtttt
    16501 ccggtccagg gccgcgaaga gctcctccgg ggaaagcccg tccgccggtc gggagccccg
    16561 gaagggcgga gtccaggggg gggtcggcgg gaggcccgga atccgggacc gtttccgtcc
    16621 gtccggggcg ggtgccccgg gcagggctgg gccggcatcg gacggcgctc ccgaggcttc
    16681 ggccgcgggc gcggcggcag cgggtctcgt ggcgggtccg gtcgcgggat ccgacgcccc
    16741 cggctcccgg tccttggcca ggacgcgcag ggcctcgaag gcgtccgcgc aggcccggac
    16801 ccgaccgggg tgcaggggag ccagctcggc ttccacgaag cgggggtcca cggcggcccc
    16861 tcccaggaga aggagggctt ccgatcccga atccccgaga gcccgggcgg ccttccgcat
    16921 ttcctccagg ctccgggtca ggagtccgga cagccccagg gcggcggccc cctccttccc
    16981 taccgcggcc aggagtcgct cgatcccgac gtcggtgccc aggtccacga cccggtaccc
    17041 cgaacccccc aggatcaggc cggtcaggtt cttgccgatg tcgtgaaggt cccccttcac
    17101 ggtggccatc accacggtgc cccggccgag gccctggacc tccgaccccg gggtcagctc
    17161 cccgagggcc gcctgggccg cctcggcgct ccggaggacc cggggcaggg gcagatcccc
    17221 cgcggagaac cgggccccaa cctcggacat ggcggaggcc acgagggagg tgaccggggg
    17281 atgcccggcg gcctcccggg ccgcccgcgc cgcggccccg gcctcgccgc gcagtatggc
    17341 cgccctcagg cgatcctccg gggctgcccc gggggtgtcc tgcgcggcgg cctcggcggc
    17401 cccgggtgcc cgcgccagga gggagtccag ggcggcggcc cgggcttcga cgcccgcctc
    17461 cccgccgatc agggtccgga cggcggagcg gatcccgacg tccacggttt ccgggtccgg
    17521 aagtcccggg tccaggatgg ccgcgtccag gccggccgcc cgggccgctt ccaggaagac
    17581 cgcggttacc gggggacgga ggggccgcgg cagtccgaag gagcagttcc ccacccccag
    17641 gatcgtcgag gaaccgggac aggcttcctt cacccggggg atcgcctcca gggtccgggc
    17701 cgcggccccc tcgaagcccc cggactcgga ggccagggag aaggtcaggc agtcgaagta
    17761 gaggtcctcc ggcgccagtc cctcctccag ggcgaggtcg tggagggccc ggcagatccg
    17821 tacctttccc gccgtgtccc gggcgggacc cccgccgtcc agggccagac agactacggc
    17881 cgccccgaag cgccgggcca ggcggaagac ccggcgggcc ttggcttcat cctccaggtt
    17941 ggcggagttc aggaggggcc gccccccgac aaggggcagg gccgcggcca ggacctcggg
    18001 gtccggggaa tccagggaca gggccgcccg ggcggtcccc gccaggcgcc cggtcagggc
    18061 cgcgaggtcc gcggcctcgt cccggccggg tcgggacaga tggaggtcca gggcatgggc
    18121 ccccgactcc tcctgggcca gggcgagggc ggcctggccc tccacgtccc cggccaggac
    18181 ccgcttggcg aaggccgcgg agcccgcggc gttggccttt tcgccgaccc gcaggaaggc
    18241 ggccggggac gcatccgccg ccttcgagac gccgccggat tccggcggcc aggcctggta
    18301 cagcgaggcc aggccgggct tccgggccgg gcgcggggag ggccggggcc ggtccgcgag
    18361 gagtcccgcc agggcccgga tgtgggccgg tcccgtaccg cagcagccac cggccaggga
    18421 tacgccgaac cttcgggcca gagcggccgt ctcccgggcg aagtccccgg ggcccatggg
    18481 gtagacggcc cgccccgctt cgaggcgggg aaagcccgcg ttgggcatca gggaaagggg
    18541 cgcgggggag gcccgggcca gggcttccag ggcggccgcg agctccgcgg gtcccccgga
    18601 gcagttgagg cccagggcca gggggcggaa aggagcgacg atggcggcca gggcctccat
    18661 gcccgtgccg gtcagcatcc ggccggaagc gtccacggtg gcggagacga tgaaggggac
    18721 ctcccggccg acctcggccc gggcgtcgaa gagggccccc agggcggcct tgacctgcag
    18781 ggggtcctgg caggtctcga tgaagaccag gtccgccccg ccttccagga ggccggccat
    18841 ctggggccgg taggactcca ccagttcggc ataggtcgag gcccccaggc tgggagggtc
    18901 ctccccgggg cccacggaac ccgccaccca gcgggggcgg ccgtcggcct cctcggcccg
    18961 acgggcggcc cgcaccgcgg cctcgcaggc ggcccggttc agctcccggg ccgcgcccgg
    19021 ggcaccggcc ttggccaggg cccgctcgtt ggcgccgaag gtggcggtct ccacgaggtc
    19081 ggagcccgcg tcgaagtagg cccggtggag ttcctccacg atttcggggg cctccagggg
    19141 caggaaggtc gcggaacgcc cgggcgccaa ctcctccagg gccgtgcccg tggagccgtc
    19201 cgccaagagg aggcggttcc cgaagaccgt ccggatatcc cggtccgaag ctcgatttcc
    19261 tccgctcatt ggatgtttcc cccgaacccg gccgcgccgc ggatccgggc cccctccggc
    19321 cccccgaaga ccgcgtccag gacgtcccgt gtggcgccgc cccggcccat ggtgaagaag
    19381 tgcagggccg gggccccgcc cgaaaggagg tcctccgcca ggcgcgccgc ggcccggatc
    19441 cccacgctcc gctcctcctc cggagttcgg gctccctcca gggatcggag caggctatcg
    19501 gggaggtcca cgaagaagtt ccggggcaca gcggcggcct gggatgcccg ggtcaggggt
    19561 ttgatcccgg ggatcacggg caccgcgatc ccggcccccc ggagggcggc gacgaactcg
    19621 aagtaccgtc gggcatcgaa gaccatctgg gtgacgatgt aggaagcccc ggcgtccacc
    19681 ttctccttga gcttccggag gtccgcctcc ttgttgggcg cggcgaagtg cttttccggg
    19741 taccccgcca ccccgacccg gaagtccgtg ggttttccag gctcagcggg ggggaggtac
    19801 tcgccccggt tgagggcggc tacatgggcc accaagtccg cggcgctgga atatccccgg
    19861 accggggatt ccggccccgc gccggcgtcc ccgggatccc ggtggaaacg atcgtccccc
    19921 cggaccacga agagttcccg gaaccccgcg tagtgcaggt cgatgaggag gtcctccatg
    19981 gcgtagcggt ctgctccccg ggataccagg tggggtacgg ctcgcacccg gaaggccgac
    20041 tggagggcca gggccgtgcc cagggtgccg ggcttggacc ggggaccgga gcgggcgaag
    20101 cctcccccgg gcaggggcgc cccgtccggg ggacccgcgg ggtggtcggt gacgctcacc
    20161 ttggagggcc cgtagggaag gagggtctcc accgccgcga agaggtcctc caggcccgcc
    20221 ccccgggcgg gagggaccac ctcgatggag aagatcccgt cggcaagcgg cacggccgcg
    20281 ttcgtatcgt tcatggaagc ctccccgccc ggaccgtccg cgtctccggc gccgccggat
    20341 ccgaccgccg tctagtctcc gaatccggcc cgcccttggg agatttacag gcccggcccc
    20401 ggcccgcagc caaagggacc agctccaggt ctcccgcgca cccgaaggtg gagcccgcga
    20461 tcagggcgat ccccaggacg ccatcggtac gccgggccag gtccagcaca ccctcgatgt
    20521 ccgccccgga tcctacacgg ttcccgaagg ccgtggccag ggcgtccgcc cgggccgcgt
    20581 cccgggccag gaccgcgcag gcgtccgcac gtccggcgct gtaggagggt cccacccggc
    20641 ccgcgctggc ggccagcccc cggtacccgc cgccccggat cctgaggccc aaggcacccg
    20701 acagggacga ggttcccgcc agcagggccg cggtgaactc cgattccccg gacagggcga
    20761 tgtccccccc gttctccacg acaagctccc ggcaccggcc ggggcccagg aggaagcggg
    20821 ccgccgcgtc cgcgaaggcc ccggccacgg cggccatggg ccccaccccg gcttcccggg
    20881 cggcgcggag catggcagac gcgatcgcgg gggcccccgg gtcatccggg atcggttcga
    20941 aggaggaccg gaattccgga cggcgctcga tataggactc cagccggagc cgaagatcga
    21001 tcacccgctc cagcacgtcc tgacccaggg aggaccgaaa ggaggggcgg tccaccccga
    21061 tccagaggtc cgtctcccgg taccgcacgg gaaagtacgc gaatcgttcc gccgccgcca
    21121 gggcgcggta ggtcctaggc tggtacacgg atgatcctct tgttcgagga cccggcgcgt
    21181 ccctccgccg ttcgcccgga tatggaaccg gccgccgccg ctgccttccc ggcgcccagg
    21241 gccttccggg gacaggcgcc gaagcaggcc ccgcagccgt cgcacaggcc ggtcacgtac
    21301 cggagcctcc actccggcgc cccgatggcc aaggcgcccc gggggcaatg ggccgcgcag
    21361 gacccgcagt ccacgcatag atcccggcgg taccccgcac ctccgacccc gggatccccg
    21421 gccgtggatt ccgccgaccc cgggatcgac acgaaggccc gctccgcgag gccccgggga
    21481 gtccggcctt ccggaagcgg ctcccggggg gcttccaggg ggaagcgacc ctccaggacc
    21541 ctttccctca ggacctccgc gatgcgccgg gccttggcca ggctcgactg gctggaggtc
    21601 cgaacccgtc ggccggccag ctccacggac cccgaccgca actccgcgta ggtgcagcgt
    21661 cccgtcacgg gccggccggg ccgggaatag tcccggacct cggtctcgat gtccccgtcc
    21721 cgcacggaca ggcggcgggc caggtcttcg tccaggaccg ggacgggcag gcccagcccc
    21781 acgaagaggc tgatcccgta gccgtcgtag cgggcggcgg ccaggaactc gggttcggcc
    21841 tccttgaggt ccgcccagag ggccaaggtc cgggccccgg ccacggggag gccggaccgc
    21901 cccgcctccc gccggtcgca gtactgggtg cccggccccg tcaccctccc ccgggccccg
    21961 cagaggaaaa ccggggtgcc cgtccccacg gtccggcact cgggatcgtt cagcaggggc
    22021 gagagctccc cggaggtgga gtacgagacg ctcccgtaat ccggctccag gagccccatg
    22081 taggtgcgca acggccggcc ggagccgttg accgcggccc cgtagttctg gtacccgttc
    22141 cggggattga agaaccaggc ctcgttcaag cggtccaggg agacggaggt ctccatactg
    22201 gtgcgagggt agcagtccgt gcccttgccc cgggcggaga gccggacttc ctctccccgc
    22261 accagggcct cgatgacgtg ggcgcccccg taccccgggt cctccggatc ctcggccgtg
    22321 gcccccaggt aggcgtccac ggcggccagg ccttcgtagg cgggcacccc gttcagggaa
    22381 atctcctcca tccggatggg cgggtccgag tgcccgaagt tcaggaagac cccgctggaa
    22441 cacatggggg cgaaggtggc ggtggtcacg acgtccacct cccgaacgag ttcggcggga
    22501 tccaggtccc gggcgaggtc cttgaactcc atggccgtca ggaccacggc atcgcctcgg
    22561 gctagcttct cgtttatctc ttcgtaggtc ttggtccgca tacgaaactc cccagggccc
    22621 cccgcacggg tcggggcggc ggccggatac ggcggcgcgc cccgacggga cggcgggcgg
    22681 cgaaatgcgg cgaccggcgg aattccgccc ggaagccggt tgtgttcgac ggtgatgttc
    22741 ggttgtaact gcccgggcgt cgtcccttcc cgcgtcgacg ggtcggaacc gcttcagtcc
    22801 taggacgccc ggggcgtcac atcatcatgc cggggaggaa ggcggggcgc atggagcgga
    22861 agcggaggag ggtgtaggag gaggagcggc cggcggggct ctgcgttcgg gtcacgggaa
    22921 cctccctccg aagcgggaca ggaacggcgc ggcggccgtt tcatcctttg aaagcctcgg
    22981 acaggcagac tataacggtt tctgtaaata agtacaagat agtgaatagg tgaatgagta
    23041 aattggtgaa taagggagga aaagttgcgc cattctccca ttctcccatt ctcccattct
    23101 cccattcacc cattcaccca ttcacctatt caccgactcc agtctatgaa ctgctccgtc
    23161 ttgggcttct ggcatccggg gcagaagaag gaatcgaagc ccaggacccc ggccttgcgg
    23221 atcgtggttc cgcacaccgg gcagggcgag cccgcccggt tccgcacccg catatggtcc
    23281 cggaccttct cctccgtggg cttccccgct tcggccacct ccgagatacc ccaggccagg
    23341 acttcccgga tcgcggtgta gaaggcgtcc ttctcatggg gggacaacct gctgcagggg
    23401 gccttggggt ggatgcgggc ggagaagagg atttcgtcgg cgtaggcgtt ccccacagcg
    23461 gaaagcttgg tctggtccat caggaagacc cgggcctggc gccgctcctt ggcgatcagc
    23521 cggtgaaaaa cctcccggtc gaaatccggg cccgtgaggt ccaccccctg ggtcaggaag
    23581 cccgggatcg cctcgaagcg gccgggttcg caaaggtaga ccttgcccat gttcttctcg
    23641 tccccgtact cgaagcgggt tccgtcctcc aggtccaggg cgaagcgtcg ggccgccagg
    23701 ggcttctccc cggccgggac aacccggacc cggcccgcca gcatgaggtg catcaccagg
    23761 tcgtgcctgt ccagggaaaa ccgcaggaag ggaccgcgcc gctccagggt ccggaaggtg
    23821 tccccggcca gaagttcccg gaaatccccg ggcaccagga tccgcaggac cgtcgggttg
    23881 cccaccgagg catcgacgat ccggcgcccc aggagggccg gccggaggcg ctctacgatg
    23941 tagaccaggt cgggaagttc gggcatgggg gaagtgtaaa acgtgaatgg gtgaatagtg
    24001 aataggtgaa tgggtattgg ctagaaatca cctagctcaa tcatgaggaa agcgcatatc
    24061 acgtagactc agggaccttc ctaattcacc tattcaccca ttcaccaatt ctctatccaa
    24121 ctccctcatc gcctccgagg cgccggcccg gatgaagatg tccgtgaccg actccgtgaa
    24181 ccccgtgggc tcggggtcga tctccaccac gaggccgccc cggcgtttca cgatccgggg
    24241 gatctcggcg gcgggataga cctccccggt gctgccgatg accaggcagg cgtccgcggt
    24301 ttccgccgcg gcgaaggacg cctcgtaggc gttccggggg atcccctccc cgaaaaagac
    24361 gaagccgggc ttgaatatcc ccccgcaccg gcaccggggc ggaagatccg ccaggagggc
    24421 cggctcggcc tcgacccggg tcccgcagtc caggcagacc aggtgccggc aggagccgtg
    24481 gaactccgcc aaggcccggg agcccgcggc ctcgtggagc ccgtcgatgt tctgggtcac
    24541 caggaaggcc aggcggccct ccgcctccca gcgggccagg atccggtggg cgtcgttcgg
    24601 ctcggccttg gaggtgtact cgtagaagat ctcccggatg gtaggccagg cggccgcggg
    24661 gtcccggagg aaacggccca gctcgagggt tgcggggtcg taccgggtcc agatgcctcc
    24721 ttctccgcgg aaggagggta tgccgctctc cacggagatc cccgccccgg tgaaggcgat
    24781 gaggcgcttc gcttgtccga gtttttgggc ggcccggagg acggcccggg aggcttccgg
    24841 agacacggag ggggaggaac ccgcggggcc gcccgccggg agagagcccc ggcccgggcg
    24901 gcgctcagcg ggaacggctt accaccaacc gaaggctgtg cccacccggg tcaggaacca
    24961 cacgaagagg atgcccacca ggatggcgta ggtccggaac cagaccgcaa agatcgcccc
    25021 ttcctgggcc gccaggacgc tgttgaatac actcgctccg atgcagacca ggaacccgat
    25081 cagggccagg atcccgtagg cgacgttgtc ctcgctcagc gccaccatgc tgaagacgaa
    25141 ggtgaaggcg aacaggagca cgaacagtcc gaaggtcacg agcccgtttc gagtatccat
    25201 acggcgactc ctttggcgtg gtgggatggt acggtaggag tataatccgc ccccgcccgg
    25261 ttgtccacga agggcgggaa aacacctccc gggcggttgc gcggccgggg cgtcgggcct
    25321 acactcgagg agagatcaag gaggggagat gaagcgagcc gtcgtattca tggccaacgg
    25381 gaccgaggag gtcgaggcga tcacccccat agactacctc cggcgcgcgg gagtcgaggt
    25441 cgacgtggcc ggtatcgggg agcgggacct gatgggcgcc catgacatcc gcctgaccgc
    25501 ggacctggcg gcggacgacc tcgaggggcc cttcgacctg gtcgtcgtgc ccggcggacg
    25561 gggctgcgag gccatcggca aggacgagag cgcccggaac ctgatcgtga agcacttccg
    25621 ggagggacgg ctgaccgcct ccatctgcgc ggccccggtc tgggtcctcg gaaagcactg
    25681 cgggatcctg gcgggccgga ggttcacctg ctacccgggc ctggaaaagg acccgtacgg
    25741 aggaaccttc gtcgccgagc gggtggtcga ggacgggaac ctgatcacct cccgggcggc
    25801 gggctgcgcg ggcgattttt ccgtagcggt catccgggcc ctgctgggca aggccgcggc
    25861 ggaggagctg gcttccaagg tcctgttgta gccgagcgcc cacggacggg cgcttgacgt
    25921 attcggccag agtcgcgaac gcgactctgg atcgtcatgg aagacgaagc acccgcgtga
    25981 cgaccggcag ggaggcggcc cgggttcccc tcttgagcag gaaggcagcc aggatcccca
    26041 gggcgaagcc cccgatgcct aaaagggccg cgggtccctc cccggaggcg gggaacaggg
    26101 ccgcgccggc cgcgtaaccc gccccgaaga ggaccagggg caggcccagg agccagaagg
    26161 cccccgcggc ctgggcgccc gaggagatct ccacctccac ccgctggccc ggggcgctca
    26221 cgagccctcc gggatcctgg gcctttatca ggctgcccgc cagtccgcag ccttccttgt
    26281 tcatgcagcc cgcgcagttc gcctccaggt ctatccgaag ggtcaccacg gacccgtcaa
    26341 tcctcgtcgt cgttccctgt tctttcaacc atgacctcct tgcagggcac cctcaacagc
    26401 tccagggaga gggccctacc gttctcgtcc gattcgacca gacagccctg gagttccagg
    26461 ttcccgcccc cgtcccgggt ccactcgggt atccccgaga ggtactcccg gacccggccg
    26521 gccgggtcca tccccccgac ggacaggaag gcccccgtgc ggcccgcgtc ggtgatcgcg
    26581 gccgtcccct tgggggaaac ccgggcgtcc gcggtgggca cccgggtgtg ggccccgatg
    26641 accgccgtga cccggccgtc caggtgggcg gccagggtga tcttctccgc tgtcgtggcg
    26701 gcatggaact ccacgatcac gacgggagtc tcctgcttca ggcgctgcag gaggtcatcc
    26761 atcaggagga agggattggc caggtgggtc cggggaaagc ccgactgccc caggagctgg
    26821 actaccgcga tcttcctctc ccccgcggcg tagaccttcc agccgtgtcc cgggactcca
    26881 tagggatagt tggccggccg gagaagccag gaggccttgg ggaagagctc gacgatgtcc
    26941 ttcttgtaga agccccgttc cccggtggtc aggatgtccg cgcctagctt ccgcaggtag
    27001 gtcgcgtggg cgcggcccac cccggcacca ccggtggccc cgtccgcgca ggccacgacc
    27061 agatcgggct tgaggtcccg ccgcaggccg ggaaggaggt tcttgaccac gaaaacgccg
    27121 gtcttgccga cgatttccgc tatgtagagg atgcgtacgc tcacccgtcc aacataccga
    27181 cggaaggcgg ggggatcaag ccgtgcgaac cgcaaagcgg ttcgcgctac tgtttctcgt
    27241 acttctccac ttcctcgtgg acgtactcga gcttgacccc ggcggtgcgg aacatggcct
    27301 cgctctccga gccggcgtgg tatttgcgct cgcagacgac ccggaggatg ccgcagttga
    27361 tgatcatcat ggcgcaggtt cggcaagggg tcatgcggca gtagagggtg gcgccccgga
    27421 tgggcacccc gttcttggcg gcctggcaga tggcgttctg ctcggcatgg acggagcgga
    27481 cgcaatgggt ggtggcggta ccgtcctcgt ggacggtctg cttgagctgg tggcccgcct
    27541 cgtcgcagtg gggcaggccg gcgggggcgc ccacgtagcc cgtggccagg acctggttgt
    27601 cccgggcgat cacgcagccg gaccggcccc ggtcgcaggt ggcccgcttg gcgatggcgt
    27661 tggtcacctc catgaagtac tcgtcccagc tcggccttcg gtactcttcc atgcccgccc
    27721 ctcccccgcg ttactccgga tctcacaaca atggaaccat agatcagaca ggattcacaa
    27781 gatggaagag gatggacagg ataaaatcgg gcatgaattt atccccaact ccaatcctgt
    27841 tcatccgttc ttcatcctgt tcatcctgtc aaaatcatgg ttcccggccc ggttttcctc
    27901 cgtgatctcc gtgatctccg tggtgaattc cctcacaacg tccgcagggc c
//
LOCUS       DAOTEI010000026        27604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1132_length_27604_cov_5.549312, whole genome shotgun sequence.
ACCESSION   DAOTEI010000026 DAOTEI010000000
VERSION     DAOTEI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 27604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27604
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_27604_cov_5.549312"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /gene="dctP"
                     /locus_tag="P5313_04130"
     CDS             <1..591
                     /gene="dctP"
                     /locus_tag="P5313_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY79586.1"
                     /translation="KSGIRVLDAWYLGTRELSLTKKAGPVRTPADLKPLKIRWPNSEA
                     WLDVGRSLGANPTPMGFGEVYMALKTGTIDGQDNPIPTSYAEKFLEVAQYIVLTDHML
                     GYVTPVINEKLWKEMPENYRFYIKQALTVARFYMNQKVMEEEATLIGKAQKEYGIQVI
                     VPDKKAFMDNAKKYYSDPKFAKRFGDGAYAKIQAVE"
     gene            693..1202
                     /locus_tag="P5313_04135"
     CDS             693..1202
                     /locus_tag="P5313_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY79587.1"
                     /translation="MKRAFKQFLDAVEVYIPAAVFALLIASIFIQVVLRYVFNSPSPE
                     LFEISIYAFVWVIYLGGALASRYDQHIRFDLIYRGLKRPAQIVVDLVFNLATLGILLY
                     LLVPSFRYTFEMYKIKASALRIPWTFLLIVYPVFILLVIIHTSTTTYRGVRELMGKGR
                     APEETPPWI"
     gene            1193..2491
                     /locus_tag="P5313_04140"
     CDS             1193..2491
                     /locus_tag="P5313_04140"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY79588.1"
                     /translation="MDIAVTVWLAVFTVSFLMGYPFGFSMFASSTLYIILKGIDITTV
                     LDIMVIQFETNFVLLAVPLFIFSAKLLNASLLTDRLFDFAKVLVGPLPGGLAHVNIVN
                     SMIFAGMSGSEIADVAGPGQMEVRAMEKAGFDRPFSCAVSASSATIGPIIPPSIPMVI
                     YAMISGASVGYLFLGGFLPGVLLGFCLMIGVYIVSIRRKYPVGRWYTFREILATTKTS
                     FLILLAPAILLLGIYTGIFTPTEAAAVVCVYSLVLSLAVYRTVNLKGLFKLLVESVAA
                     AGFTSFTIGGAFLFGYVLAREEIPAKIAEFFLSLGISGSPALTLLSINVLFLILGCFV
                     DVSVSLLIVVPLILPVVEAAGIDLVHFGVVVTLNLMIGLSTPPFGEAVFIIARVTDTP
                     LGAVFKEMWRFIGFMILALMLISVFPDIVLFVPRLAGYQG"
     gene            2495..3700
                     /locus_tag="P5313_04145"
     CDS             2495..3700
                     /locus_tag="P5313_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79589.1"
                     /translation="METDERFIDALREGLDEETRARTDRVADTAAKIKERGGKIAVVT
                     GSGPNLHEGVTTLLAEFIRLGVVDGVLTSSAVVAHEMAGALDEVKRVPGSAVGIPPEL
                     LPKGGIFEASLLRPGDLDRISREMVLDRGLIDRVLAAEGRVIIKAAGNMAYPVGLRTE
                     RLAMEIESLADLHGLPFERAAGLGADPRTMIGAGAAAGVPVLVTVPQLVGGGMVGLAV
                     GDSIPLKRRSELVSRILGGADLIIESGIALSQEIHDGPFETYTGHGIWSRWEGSPTYS
                     LAGKTLVRIDLDPNLERVWRIEREGASVQGAVDQGLPKTKTFKVPFRMEMSGFARLEG
                     SLPLVGDLGILWPVIALGIARRLGLKPEFMSYPQESEPGKRMRRWIVDEVGILDRDRM
                     LAGLREARP"
     gene            3697..4434
                     /gene="kdsB"
                     /locus_tag="P5313_04150"
     CDS             3697..4434
                     /gene="kdsB"
                     /locus_tag="P5313_04150"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRY79590.1"
                     /translation="MNGIVGVIPARYASTRLPGKPLADILGKPMIRRVYESAARARVL
                     DRLLVATDDERIAMVVRAFGGEAVLTSPDHPSGSDRIAEAIRDVRADIVVNIQGDEPF
                     VSPTMIEEAVRPLLEDPTLPMSTLMHEIPEESFGDPNVVKVVVNLRGEALYFSRSLIP
                     FPRKRDGHRAYEHVGIYAYRAEFLRTFTQLPPSPLERFESLEQLRVLENGYRLKVVET
                     RAPDYVPLSIDTVEDLERARKLAEKLG"
     gene            4512..5570
                     /locus_tag="P5313_04155"
     CDS             4512..5570
                     /locus_tag="P5313_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY79591.1"
                     /translation="MLKLDEKLLAANKETEILSAYIAADRIEAGLAENREVFDDLAAY
                     IRSCRPDRLVFVGSGASWCSLYTGFWFLRSSSSLPVLHFFGPELVADDPPVLREGRTL
                     AILSSYSGKTADTLAASEFLGARGIPRIALSKDPGGPLARSCERVLAYGDKCLYTSAM
                     ANLLSLLGAYLRLRGEESQAEGMLADLARIPDLMRGSLRKAEERAMAALEPLAGDDFF
                     YVLGDGALWALAYQYGYTNLTEYSRVNAACLRACEWRHGPLEVLFRKPAMVHFIGNDR
                     TRAYSLATKEYCAANGARLAVFDVDDYYRVHPALAPFVLHPVSQFFLLYQSTQRGIDM
                     DDYLQMHVKPYLPGETYF"
     gene            5570..6529
                     /locus_tag="P5313_04160"
     CDS             5570..6529
                     /locus_tag="P5313_04160"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY79592.1"
                     /translation="MGDFKGVYAAMLTPLGEGGAAPSARTVESYCRFLLERGVSGLFP
                     LGTTGEWPLLREEERMRIAELVVGSAKAWRDDPARGARPGADSGPAKVIVHAGADSTD
                     ETVRLCRHARGIGADAVGVICPPYYPLNEEALRFHFITAARSVPDLPFFVYFIPSLVR
                     CDMTPALLLNLAREADNIVGLKYSGESIPRFREYRRVLGPSFSLFIGDDNHALPGLRE
                     GADGIVSGNASAMPELLVDLYRSFRAGEDEAATEAQRELDDFIAGIDGRAELSSFKRI
                     LGIRGVPAGEVRRPLNPLSPDGSEALAARIRRLEERNKARKPL"
     gene            6526..7527
                     /locus_tag="P5313_04165"
     CDS             6526..7527
                     /locus_tag="P5313_04165"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY79593.1"
                     /translation="MSETIFLTGASGRIGGRVAAGLLERGYKVRALIHSNRGNLPDGS
                     GLVTLEGDIVTRKGLAEGVEGADFVIHLAAAWDMFPPARYEATNDQLFESVLRGTYNV
                     LEAARSVRGLRALVYASTDAVYATGPRKFEAPITEDTELQPSRFYALCKIACETLCRQ
                     YGRLYGLPWIVVRICWSLEPAELLRVFGYDFWEGGMSEEDRARLKPILGGEKGVFAPL
                     NEDGSSGVDHIADPDDIAAGIVQAVVNHDKGLGGTFNLAGPAPFRYLEVIEKLAADLG
                     VPWGSARIPGIHPYELRNDRAIRTLGYAPAWTMERMLEKAAAARRTAAGKNPAGGTT"
     gene            7524..8540
                     /locus_tag="P5313_04170"
     CDS             7524..8540
                     /locus_tag="P5313_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRY79594.1"
                     /translation="MKAAVLYGKGDLRMEDRGVPVPGAGEVLVRIRACGVCGTDAALF
                     RGEYPAAFPVVIGHEFSGEVAALGKDVGGFKVGDRVTADPNVLCGSCGYCRGGLGHLC
                     SRVSSMGVHRDGADAEYALVPAVNLYTFPETLAFEEAAFTEPLACAVHGVDLAGVRTG
                     DTVLVLGAGGMGNLIAQLARLSGAARVVVSEPIAMRRDRALENGATDAIDPGAGDVGE
                     ALRRINPLGADVVFEVAGHVGLQAQAVRLARKGGTVVWFGVAPPDKVAEVSPFYVNEN
                     EIRILGSYNNPFATDRAVKLLAERRIRVDNLITHRIALGDYPKALSLFGGRDTVKLMV
                     TMGA"
     gene            8537..9511
                     /locus_tag="P5313_04175"
     CDS             8537..9511
                     /locus_tag="P5313_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRY79595.1"
                     /translation="MMERIVDIVGNLNMDLILRGIPEMPAWGREVFGNDCLLVPSGQA
                     GYTALALGALGVPVRVHSVLGGDPWGDSILAALDGSGADVRGIERAAGTRTGLSVALV
                     RGDGERAFASYYGSLAAFDAQALGRLHPGIRGRGALLVCGINALPNLPLDAVEEICRD
                     YRGSGGLTALDTGWDPLGWGPERLAGLRRLLREISVFIPNEEEARAMTGFEEPEEQAK
                     ALLGFGAGLVVIKLGPRGCYAAGGGSAERLPAFPAEVLDAVGAGDVFNAGFLAATLSG
                     RSLRERMAYSSAAASIYISRAENRYPSARETAAVAESLGAPGAVKEGT"
     gene            9508..10368
                     /locus_tag="P5313_04180"
     CDS             9508..10368
                     /locus_tag="P5313_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY79596.1"
                     /translation="MIDFHTHPVMIKELVEADPDFGRAVREVFGLLFPPQPLEILRRE
                     MDAAGIERAVILALDLSTAHGCRVPDNETVARLAESEPRLIGFASVDPNLPDAAERLD
                     GAVRRLGLRGLKLDPSLQRFDPGDRSRAYPLYRLAGELGIPVLLHAGMSWAPPGVSRL
                     SRPVLLEEALSDHPGTNFVLAHFGWPWTDEALMLALKHRNLYLDTSVVYARTPAETLR
                     KVVAEHIGLPVLEGSLPDRILFGSNYPRQDMRRAARGIASLGFSPGLLEAITRGNAAR
                     LLGLETEARP"
     gene            10365..10814
                     /locus_tag="P5313_04185"
     CDS             10365..10814
                     /locus_tag="P5313_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRY79597.1"
                     /translation="MISHLKRFTILTRKETEIIDITPSVLETVAASGVWSGTVFVLSL
                     HTTTGFYVNEGLPDLERDMADFLARLVPEDHPYRHARFLHSDGQMAINAPSHLRGTML
                     GFQAFFPVEEGKLVRGGRQTMYLAELDGPQERTYVVQVTGEARGGEA"
     gene            10811..11725
                     /locus_tag="P5313_04190"
     CDS             10811..11725
                     /locus_tag="P5313_04190"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY79598.1"
                     /translation="MTEPKWAEGLEGVIGALAVPMFEDGSIDYSLLEKQAAYYGGSGV
                     RGLFLGGTNGEGAYLTTEEKAHILKTVRGAVPKDKILCAACIRPSTREVLAEMRVLGA
                     LGPDYIVAVAPYYLAVDQEAIIRHFRTLAAASPAPLIPYNIPQNTHNPMSLKTIQELA
                     SIPNIAGIKDSSGDFISFTRGVLGPLPGPFTWIQGEDLLDAPSYLIGAKAVVTGLSNV
                     SVEPYVDMWRAATAGDTEGVKAGQRRINEMFKIVLAVGGKGIPAIKAAMELLGRGSRH
                     LRLSALTLDDAEVEAVRETLVGMGLLRR"
     assembly_gap    11866..11965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12151..13500)
                     /locus_tag="P5313_04195"
     CDS             complement(12151..13500)
                     /locus_tag="P5313_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRY79599.1"
                     /translation="MIQNLLAMSVPTMLGMLGQTLYDVVDLFWIGRISGSAVAGVALF
                     SSIFWLVEVLNEVIGVSSIALITQSFGAGDKARTARVIEQTIVFKSFMAVIASAFLYF
                     ALEPLIRFFTSDPEVIRSALEYGRIRTFFLPVFFATYSCFTALRCTGDPKSQMWIMLG
                     VSILNAVLDPLFMFSEVPYFGVRGLGLGVFGAGLATVLCICAAFAVGFGLLASGRTRI
                     KLSVRGLFRLDPAIDRKLITIGLPSGGEMLARQAAGLVTTKFVARYGTAALAALGIGN
                     RLAGLVFMPLFGLMGGGGTIVGQNLGAEQVDRAERTAKAAALLGGITITTLVALACAF
                     PRSVLGFFVDSAETVAVGVPMIRFLGPSFAIVSFAIGLGCAFTGSGYNIPYLISSLAG
                     RWGAQVPFLALSAFVLPRLGLDLGILGIWASFLVSDSVEAAVLAWHYRKGVWKGKRV"
     gene            complement(13613..15919)
                     /locus_tag="P5313_04200"
     CDS             complement(13613..15919)
                     /locus_tag="P5313_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY79600.1"
                     /translation="MEKGIEVQSILKSLEPEEAASDARPDDSIFTQLLERLVQGYSLR
                     IESGEDEADSLEILLDGVSDSVLIVSPSGAILNANPSFYRTFGYDREELLDSEIYALL
                     PPSHRDVFRQKFAAFGLREGERHSASSDDIMAFRGLRRDGVTLTLDCLLSSVQCGGQP
                     AVLMLIRDLSFDHALFEQLKETKDHYVALSETITEAIFRLDENFSIIFANSGVKNTFG
                     FEREDVVGKPFRVLFPPEVFAQHEKEFNKYFFVDDADRLAQGMKRTLEILGATKNRGV
                     APMEMSFGNSKDYKGRSLTCVIRDITQRKTLERRLRHLAYHDKLTGLGNRDLFNEDMK
                     SVLDRVRDDPEWKGAVLFLDLDGFKRVNDTLGHAAGDELLIETGRRLRVCLRDRDSAY
                     RFGGDEFVAVLGGIKETGDAAIVAERILATVGNPYILRTKGKNAGGKVNVGVSIGIAV
                     IPEYGATLEEATRNADIAMYCSKEAGKNRYTLYDPVITAKATEHWKMEQELAAAMTSG
                     GLSLAYQPIVGPDGRVRGMEALLRWRRPDGRMVPPSEFIPLAEANGTVMALGEWALRK
                     ACYDARRLHARGHTDVFVSVNLSGRHFDQPNLVPTLAAIVRQSGLPPGNLCIEVTETA
                     IMKNTQDAIERIKEAKRLLPGIRFMIDDFGTGYSSLAYLSRLPVDALKIDISFVTQLA
                     EPQNQKIVNAILNLARNLQLDTIAEGIETREEWQYFARRKCGAMQGYYILRPSNLGKV
                     AALIERQAREARPTVSAETAAKTVSDSD"
     gene            complement(16039..17175)
                     /locus_tag="P5313_04205"
     CDS             complement(16039..17175)
                     /locus_tag="P5313_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79601.1"
                     /translation="MKANGIPSIRNGALCCLAVLFLQGAAASQKPAAVLEYYGEIGCS
                     HCDTFEEKILPAAAARAGAAVELIQVDILSASGYERCRARLAEFGQEFRIFPVLVIGN
                     NAYQGNAAIEENLLPELEHFVREGGFRPRRDPSGERALSPGMRLEFLPVLLAGLVDGI
                     NPCAFTTLLFFLSFVSLGGAGRGRIAGIGLLFAAGVFLAYLLLGLGLFNLLRSGIRMA
                     GFRLALRILVTGLTAAFCALTVRDLLLLRGGKAADMKWGLPEGARKGIHAAIRGGTRG
                     AVLPAGVFLTGVVVAVLELACTGQVYFPTLSYMAQTGGGWLGIGGLLTYNLAFILPLL
                     AVLGLILLGVRQERIRRFFEARIALAKGLLAMTFAGLAALVWIF"
     gene            17282..17938
                     /locus_tag="P5313_04210"
     CDS             17282..17938
                     /locus_tag="P5313_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY79602.1"
                     /translation="MLVQELMTKNPLTTTPDTSVPDALALMRQKKVRRLPVLDKTGKL
                     VGIVSDQDLLYASPSPATSLSVFEINSLIAKITVAHVMTKKVVTITEDLPVEEAARIM
                     ADKKIGGLPVMKGTKLAGIVTETDLFRALLQLLGGRRSGVRVTVQIPGAKGTFAKISG
                     IVSAAGGDIVGLGVTEAQGVPGDFWHMTLKVQDLTKKAVSAAFAKAGVEVVDIRETKE
                     "
     gene            complement(18221..18763)
                     /locus_tag="P5313_04215"
     CDS             complement(18221..18763)
                     /locus_tag="P5313_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY79603.1"
                     /translation="MNRDLELVPGFYSETDDTLDLVLEPLDGVERGAVIRMSGYLDGF
                     NVGFFSKRVEKVIDRGFLFLSFDLGNVTYISSRGIGAFTAFLKMLKSRGGNIHLHNVQ
                     PKVLEVFRILGFLEYFNIGTSREEAARGFRSAGAAVFPLRVACPVCGKALRVPGAGRG
                     RCPACKCLLAIDACGRVTRS"
     gene            18937..19656
                     /locus_tag="P5313_04220"
     CDS             18937..19656
                     /locus_tag="P5313_04220"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HRY79604.1"
                     /translation="MFREVCPACRRPLPVCLCPDEPPMETRTRIVLLTHPKEWKRQRS
                     GTGRLTSLHLRNCEIVPGLRFDEHPRIRSLLEDPEGYPVLLYPGPGAWNLSEREFPAK
                     ALEGRRLTVFLVDATWACSRAVLRESPGLSRLPRVMFTPREPSRWVIKRQPRPECLST
                     LEAVHELLTALDAAGLDEYPDRTRLLEAFRRMQEIQVECARTRGRARFRGYDRVDRLL
                     EAEGEAGEPAVDLPELRGVHR"
     gene            complement(19588..20358)
                     /locus_tag="P5313_04225"
     CDS             complement(19588..20358)
                     /locus_tag="P5313_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRY79605.1"
                     /translation="MFRYAVVRRPARSLVSGLTSSPHLGTPDYEKALRQHDDYIRALE
                     ACGLSVTVLEALEEYPDSCFVEDTAFCLPDRAVLTNPGASSRKGEVEDMERALRSFYT
                     PDRIFRIRAPGTLEGGDILRAGGEVFVGLSDRTNQEGAIQFLNLLRDWGIPGQPVPVT
                     GVLHLKTGLSFLEGGRLLVTEEFAEYPPFRDFEKIPVPEAEAYAANCLWVNGNVLVPA
                     GFPGIRTALEDLGYPVLSVDTSEFRKIDGGLTCLSLRF"
     gene            complement(20510..22186)
                     /locus_tag="P5313_04230"
     CDS             complement(20510..22186)
                     /locus_tag="P5313_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY79606.1"
                     /translation="MNPPSAREILEIFYPYVAVNSESGTARENLAAAFFRAHFEKSPR
                     FAGGPGFFGTHPVPADPAGRTVEWALVRGRGKRTAVLMHHFDVVEIEDYGPLGHLAFD
                     PERLAAALRENPGSLDDESRADLESGGFIFGRGAADMKAGGAIQMALLDEAARDPYFK
                     GNLLLIALPDEENLSAGMRAAVDLLADLKGRYGLDYVLMINSEPHQRKNADTGILSGG
                     SIGKILPFVYVRGILAHAGKSAEGFNPLGILSRIVRGTEMSTELADCLEPEPEMSPPP
                     TWLMARDSKEAYDVSMPLSAFGILSVQPLENRPGRVRESLLRICEAAAGEASETAVRS
                     SRLFRSRTGRAVEESPAWTGDRKPRVLSFGSLARELRSKDEALFEKTREAAAERVRRG
                     EPLFRAVWSLVDALAGGLGGGRPLVVTGFLPPYYPSVSHRDRPDFDAAIRSLAARLSG
                     RARELWGQEYELESYFTGISDLSYSSILDAEGIETLIRDEMPLYGDAYSIPFGRIAGI
                     SMPCVNIGPWGKDFHKRTERVSRQDLTERTPVLLAEAVAGVLDPAGKDPF"
     gene            complement(22498..22571)
                     /locus_tag="P5313_04235"
     tRNA            complement(22498..22571)
                     /locus_tag="P5313_04235"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22535..22537),aa:Ala,seq:ggc)
     gene            22702..23853
                     /locus_tag="P5313_04240"
     CDS             22702..23853
                     /locus_tag="P5313_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRY79607.1"
                     /translation="MPKSNEAYAAICPVGLEKVLAQELSRMGIEAESRDPGRVGFRAD
                     ARGVASALCALRTADRILAAPGGFHAPDFDSLFEGMRALPWERWIGREDRVTIVRVRS
                     RASALGAQTSVQAVAHKAVYERLGRVYKVSRMPQTGPEREVRLYLDDDLCTPLLDLSG
                     DPLYKRGWRTESVEAPLRETVAAGLLFLSGWTRKRPLRDPFCGGGTLAVEAALWARDE
                     APNLRRRFAWETMPSFDPEALQGVRADLEGRIRRDIRADILGSDRDEGAVSVARANAR
                     RAGVEDLVRFERLDMEDSSSGGLPEGLVLANPPYGLRMGTEAEAEVLWRAAGGLKDRY
                     PGWSFGFIANREDFPAFFGARPASSRTILAGAEKLWFHWFPAASGSGKG"
     gene            23974..24933
                     /locus_tag="P5313_04245"
     CDS             23974..24933
                     /locus_tag="P5313_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY79608.1"
                     /translation="MKRSIAILGIAVLLVAALLIGCQPKEVKPTFINIATGGTAGTYF
                     PLGGALAEIWNTNIKGMNATAQSTGASVANINLLRDGKNEVIMVQNDICYYAVNGLEM
                     FKDKAFADIRGLVTLYNETCQVVTLADKGINGIADLKGKRVAVGAAGSGAEANARQIL
                     EAAGLSYSDLTAQYLSFAEAANNLKDGNIDAAFVTAGHPTAAIQDIAASRKVALVNIS
                     PEIITAITSKYPYYTPTTIPAGTYTGVDVPVSTVAVKAMLAVKADLDAKVAYEMVKTM
                     YANTERLKAAHKVGSQITVDTGKAGMPLTLHPGAEKFFKEAGK"
     gene            25040..25537
                     /locus_tag="P5313_04250"
     CDS             25040..25537
                     /locus_tag="P5313_04250"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HRY79609.1"
                     /translation="MRRPIRLFALAAVLVLIAGYALLPRTPTLTIRNERGRILARLAC
                     EDGRFVHRYVHSVHLSDVDEEYSIESDGSLRLTATRFDTLGVGIPYDAEGGFSKDGNR
                     FVLRMDRRYRSLPVRVSPIPGHVIVTGGRIFPLSEWVGPGELAVLEARNIPRTGILTP
                     RREDR"
     gene            25674..>27604
                     /locus_tag="P5313_04255"
     CDS             25674..>27604
                     /locus_tag="P5313_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRY79610.1"
                     /translation="MEAADLSDTVNGAEILKKFDKESDFRTLACFLGKFIAALAIAFS
                     VFQVYTAAFGVLDAMIQRAIHLGFGLTLIYLLYPTSKKWPRDKLHPVDALLAVASAVA
                     PMYILITYQQLVLRAGTTTPMDIAMGLLGIILVFEAARRVVGLPIVIIAGIFVLYAFA
                     GPYIPGRLAHRGARLDTLVGHLFFTTEGIFGIPLGVSSTFIFLFILFGAYLEKTGLGQ
                     YFIDIANSIAGWASGGPAKVAVLSSALMGTVSGSSVANVVGTGSFTIPMMKKLGYKPE
                     FAGAVEATASTGGQIMPPIMGAAAFLMAEFTGIPYARIIGAAVVPALLYYFGVWAGVH
                     IEAKRLGLRGLRKDELPKLGELLKKSFLMLPLAAIIYLLVSGYTPMRAALIAIVLSIG
                     TAGAASLVNWSRKRPVGFRWVDIVKGLEAGARGALGVLAATACAGIIIGVVTLTGLGL
                     KLGSSLVALANGQLFLTLVFTMFTSLILGMGVPTTANYVITSTIAAPAIMMILTKVNP
                     GMDPYAQAIVLPAHMFAFYFGIIADVTPPVALAAFAGAGIAKANPMKTGFNATKLAIA
                     AFLVPYIFVYSPQMLGIGATFLDMVGIFVTSSIGMIGVSAGVMGYLIRPMHLWERPIL
                     FIGGILMVIPGAVTDAIGLA"
BASE COUNT         4702 a         9378 c         8874 g         4550 t
ORIGIN      
        1 aagtcgggca tccgggtcct ggacgcctgg tacctcggca cccgcgagct ctccctcacc
       61 aagaaggccg gcccggttcg gactcccgcg gacctcaagc ccctcaagat ccgctggccc
      121 aattccgagg cgtggctgga cgtgggccgc tccctcggcg ccaacccgac tcccatggga
      181 ttcggcgagg tctacatggc cctcaagacc ggaaccatcg acggccagga caaccccatc
      241 ccgacgagct acgcggagaa attcctcgag gtcgcccagt acatcgtcct gacggaccac
      301 atgctcggct acgtcacccc ggtcatcaac gagaagctct ggaaggagat gcccgagaat
      361 taccgcttct atatcaagca ggccctcacc gtggcccggt tctacatgaa ccagaaggtc
      421 atggaggagg aagcgacgct gatcggcaag gcccagaagg aatacgggat ccaggtgatc
      481 gtgcccgaca aaaaagcctt catggacaac gccaagaagt actattcgga cccgaagttc
      541 gccaagcgct tcggcgacgg ggcgtacgcg aagatacagg cggtggaatg aggctttcgc
      601 ggacctgacg cgatagaccg acctttgaat cgagcgtcgc gggcgaccgg tacagtcacc
      661 ggccgcccgc gacgcgacag cccggaggca cgatgaagcg agcattcaag caattcctcg
      721 acgcggtgga ggtatacatc ccggctgccg ttttcgccct cctcatcgcg agcatcttca
      781 tccaggtcgt ccttcgatac gtcttcaact cccccagccc ggaactcttc gagatatcga
      841 tctacgcctt cgtctgggtc atctacctgg gcggggccct cgcctcccgc tacgaccagc
      901 acatccggtt cgacctgatc taccgcggcc tgaagcggcc cgcccagatc gtcgtcgatt
      961 tggtcttcaa cctcgcgacc ctcggtatcc ttctctatct cctggtgccg agcttccgct
     1021 acaccttcga gatgtacaag ataaaggcgt ctgccctgcg gatcccctgg acctttctcc
     1081 tgatcgtgta ccccgtattc atcctcctcg tgatcatcca caccagcact acgacatacc
     1141 gcggcgtccg ggaactcatg ggcaagggcc gggctcccga ggagactccg ccatggatat
     1201 agccgtaacc gtctggctcg ccgtcttcac cgtcagcttt ctcatgggat accccttcgg
     1261 gttcagcatg ttcgcgagct cgaccctcta catcatcctc aaggggatcg acatcaccac
     1321 cgtgctggac atcatggtga tacagttcga gaccaacttc gtgctcctgg cggtgcccct
     1381 gttcatcttc tccgccaagc tcctgaacgc gtccctcctc acggaccggc tcttcgactt
     1441 cgccaaggtc ctggtgggcc ccctgccggg agggctggcc cacgtgaata tcgtaaacag
     1501 catgatcttc gcgggcatgt ccggatccga gatcgcggac gtggcgggtc ccggccagat
     1561 ggaggtccgg gcgatggaga aggccgggtt cgaccgcccc ttcagctgcg cggtctcggc
     1621 atcctcggcc acgataggcc ccatcatccc gccttccatc ccgatggtga tctacgcgat
     1681 gatctccggg gcctccgtcg gctacctctt cctggggggc ttcctccccg gagtgctcct
     1741 ggggttctgc ctcatgatcg gggtctacat cgtgtcgatc cggaggaaat atccggtggg
     1801 ccggtggtat acgttccggg agatcctcgc gacgacgaag acgtccttcc tcatcctgct
     1861 ggctcccgcg atcctgctcc tgggcatcta caccgggatt ttcacaccca ccgaagccgc
     1921 ggcggtggtc tgcgtgtact ccttggtcct cagcctggcg gtctaccgga cggttaacct
     1981 caaggggctt ttcaagctcc tcgtggagag cgtggccgcg gcgggcttca ccagcttcac
     2041 cataggcggc gccttcctgt tcgggtacgt cctggcccgg gaggagatcc cggccaagat
     2101 agccgagttc tttctctccc tggggatctc gggcagcccg gccctcaccc tcctttccat
     2161 caacgtgctt ttcctcattc taggatgctt cgtggacgtc tccgtgtcgc tgctcatcgt
     2221 ggtgcccctc atcctcccgg ttgtggaggc ggccggcatc gacctggtcc acttcggcgt
     2281 cgtggtcacc ttgaacctca tgataggact gtccacaccg cccttcgggg aggcggtgtt
     2341 catcattgcc cgggtgaccg atacgcccct gggcgccgtg ttcaaggaga tgtggcgctt
     2401 catcggcttc atgatcctgg ccctgatgct catcagcgtg ttcccggaca tcgtgctgtt
     2461 cgtaccccgc cttgccgggt accaaggata acctatggaa acggacgaaa gattcatcga
     2521 cgccctccgg gagggactcg acgaggagac aagggcccgt accgaccggg tggccgatac
     2581 ggccgcgaaa atcaaggagc ggggcgggaa gatcgccgtg gtcaccggca gcggacccaa
     2641 cctccacgag ggggtgacga ccctcctggc ggagttcatc cgcctgggcg tggtcgacgg
     2701 ggtcctcacc tcctcggccg tggtggccca cgagatggcc ggagccctgg acgaggtgaa
     2761 gcgtgtcccc ggatccgccg tgggaatccc cccggagctg ctccccaagg gagggatctt
     2821 cgaggcgagc ctccttcggc ccggcgacct ggatcggatc tcccgggaga tggtcctgga
     2881 ccgcggcctg atcgaccggg tactggccgc ggaaggcagg gtcatcatca aggccgcggg
     2941 caacatggcc tatcccgtgg gtctccggac cgagcggctc gcgatggaga tcgagagcct
     3001 ggcggacctc catggtctgc ccttcgagcg ggcggccggc ctgggcgccg acccccgcac
     3061 gatgatcggc gccggggcgg cggcgggcgt tcccgtcctg gtcaccgtgc cccagctggt
     3121 gggcgggggc atggtcggcc tggccgtggg cgactcgatc ccccttaagc ggcgctccga
     3181 actggtgtcc cgcatcctgg gaggcgcgga cctgatcatc gagagcggca tcgccctttc
     3241 ccaggagatc cacgacggcc ccttcgaaac ctacacgggg cacggaatct ggagccgttg
     3301 ggagggctct ccgacctaca gtctggcggg caagaccctg gttcgcatcg acctggaccc
     3361 gaacctggag cgggtctgga ggatcgagcg ggagggggcc tcggtccagg gagccgtgga
     3421 ccagggcctc ccgaagacca agaccttcaa ggtaccgttc cgcatggaga tgtcgggctt
     3481 cgcccgcctg gagggcagcc ttcccctcgt gggggacctt ggcattctct ggccggtcat
     3541 cgccctggga atagcccggc ggctcggcct caagccggag ttcatgtcct atccccagga
     3601 atccgagccc ggcaaacgca tgaggaggtg gatcgtggac gaggtgggga tcctggaccg
     3661 ggaccggatg ctcgcggggc tccgggaggc gcggccgtga acgggatcgt cggcgtcata
     3721 cccgcccggt acgcctccac caggcttccg gggaaacccc tggcggatat cctcggaaag
     3781 cccatgatac gccgggtcta cgagtcggcg gctcgagcgc gggtgctgga caggctcctg
     3841 gtggcgaccg acgacgagcg catcgccatg gtcgtccggg ccttcggggg ggaggccgtc
     3901 ctgacatccc cggaccaccc ctccggctcg gaccggatag cggaggcgat acgagatgtc
     3961 cgggcggaca tcgtcgtgaa catccagggc gacgagccct tcgtctcgcc gaccatgatc
     4021 gaggaggcgg tccgtcccct cctcgaagat cccacccttc ccatgagcac cctcatgcac
     4081 gagatccccg aggaatcctt cggggacccg aacgtggtga aggtcgtcgt gaacctccgg
     4141 ggcgaggccc tttacttctc ccgctccctc atccccttcc cgcggaagag ggacggccat
     4201 cgggcctacg agcacgtggg catctacgcc tatcgcgccg aattcctgag gaccttcacg
     4261 cagctccctc cctcgcccct ggagcggttc gagagccttg agcagctccg ggttctggag
     4321 aacggatacc gcctgaaggt cgtggagacc cgggcgccgg actacgtccc ccttagcatc
     4381 gacaccgtcg aggacctcga gcgggcccgc aagctcgcgg agaagctcgg gtagcgtccg
     4441 ccgggcggcg gaccgtacat cgcgtgaaac gggcccgggc gggcccggca tactatggtt
     4501 aggagaatcg gatgctgaag cttgacgaga agctccttgc ggctaacaag gagaccgaaa
     4561 tcctctccgc ctacatcgcg gcggatcgaa tcgaggcggg gctcgcggag aaccgggaag
     4621 tcttcgacga cctggcggcc tacatccggt cctgccgccc ggaccgcctc gtcttcgtgg
     4681 gcagcggcgc ctcctggtgc tccctgtaca cggggttctg gttcctcaga tcctcgagct
     4741 ccctgccggt cctccacttc ttcggcccgg aactcgttgc cgacgatcct ccggtcctcc
     4801 gggaaggccg gaccctggcc atcctctcct cgtattcagg aaagaccgcc gacaccctgg
     4861 cggcgagcga attcctgggt gcccggggca taccccggat cgccttgagc aaggatccgg
     4921 gaggtcccct ggcccggtcg tgcgagcgcg tgctcgccta cggggacaag tgcctgtaca
     4981 cttcggccat ggccaacctc ctttccctcc tgggagcgta cctccggctc cgtggggagg
     5041 aatcccaggc ggaggggatg ctcgccgacc tggcacggat ccccgacctg atgcgcggct
     5101 ccctccgcaa ggcggaggaa cgggccatgg cggccctcga gcccctggcc ggggacgact
     5161 tcttctacgt cctcggggac ggagccctct gggccctggc ctaccagtac ggctacacca
     5221 accttacgga gtactcgagg gtcaacgcgg cctgcctccg ggcctgcgag tggagacacg
     5281 gccccctgga ggtcctgttc cggaaacccg cgatggtcca cttcataggg aacgacagga
     5341 cacgggcgta ttccctggcc acgaaggaat actgcgccgc gaacggcgcg cggctcgcgg
     5401 tcttcgacgt ggacgactac taccgcgtcc atcccgccct ggcccccttc gtcctccatc
     5461 ctgtaagcca gttcttcctc ctataccaga gcacccagcg agggatcgac atggacgact
     5521 acctccagat gcacgtgaag ccctaccttc cgggagagac gtacttctga tgggcgattt
     5581 caagggcgtt tacgcggcga tgctcacccc cctcggcgag ggcggggcgg caccgtccgc
     5641 ccgtaccgtc gagtcctatt gccggttcct cctggaacgg ggcgtgtccg ggctcttccc
     5701 cctgggcacc acgggcgaat ggcccctcct ccgggaggag gagcgcatgc ggatcgccga
     5761 actcgtcgtg ggatccgcga aggcgtggcg ggacgaccct gcccgcggag ctcggccggg
     5821 tgcggactcc ggtccggcca aggtcatcgt gcacgccggc gccgactcta ccgacgagac
     5881 tgtacgcctc tgccgacacg cacgcggcat cggcgcggac gcggtcgggg tcatctgccc
     5941 gccctattac cccctgaacg aggaagccct ccgtttccat ttcattaccg ccgcccggtc
     6001 cgtgcccgac ctgccgttct tcgtatattt tatcccgtcc ctggtccgct gcgacatgac
     6061 cccggctctg ctcctgaatc tcgcccggga agcggacaac atcgtaggcc tcaagtactc
     6121 cggggaaagc atcccgcgct tccgggaata ccggcgcgtc ctgggaccct cgttctccct
     6181 gttcatcggc gacgacaacc acgccctccc gggcctgagg gagggcgccg atggcatcgt
     6241 gtcgggtaac gcctcggcca tgcccgagct cctggtcgac ctctacagga gtttccgggc
     6301 cggcgaggac gaggccgcga cggaagccca gcgggaactg gacgacttca tcgcggggat
     6361 agacggacgt gccgagctgt cgtccttcaa acgaatcctg ggcatccggg gagttccggc
     6421 cggagaggta cggcgccccc tcaatcccct ctcccccgac ggatcggagg cactcgccgc
     6481 gcgaatccgc cgcctcgagg aaaggaacaa ggcaaggaaa ccactatgag cgaaaccata
     6541 ttcctcaccg gcgcgtccgg caggatcggc ggcagggtcg cggccggcct cctcgagagg
     6601 ggctacaagg tccgcgccct gatccactcg aaccggggga atctcccgga cggctccggc
     6661 ctggtgaccc tggaagggga catcgtaacc cggaagggtt tggcggaggg agtcgaaggc
     6721 gcggacttcg tgatccacct ggcggcggcc tgggacatgt tccctccggc ccggtacgag
     6781 gccacgaacg accagctctt cgagagcgtc ctccggggca cctacaacgt cctggaagcg
     6841 gcccggtccg tccgggggct ccgggccctg gtctacgcct ccaccgacgc ggtctacgcc
     6901 acgggacccc ggaaattcga ggctccgatc acggaggaca cggagctcca gccctcccgc
     6961 ttctacgccc tgtgcaagat cgcctgcgag accctctgca ggcaatacgg aaggctctac
     7021 ggtctcccct ggatcgtggt caggatatgc tggtccctcg aacccgcgga gctgctccga
     7081 gtcttcggct acgacttctg ggaaggcggg atgtccgagg aggaccgggc gcgactcaag
     7141 cccatcctcg ggggcgagaa gggggtcttc gcccccctca acgaggacgg aagttcgggg
     7201 gtcgaccaca tcgccgatcc cgacgacatc gccgcgggaa tcgtccaggc cgtggtcaac
     7261 cacgacaagg gcctgggtgg aaccttcaac ctcgcgggtc ccgcaccctt ccggtacctc
     7321 gaggtgatcg aaaagctcgc cgcggacctg ggcgtcccct ggggttccgc taggataccc
     7381 gggatccatc cctacgagct ccggaacgac agggcgatcc ggacgctggg ctatgccccc
     7441 gcctggacca tggagagaat gctcgaaaag gcggcggccg cgcgccgaac cgcagccggg
     7501 aagaacccgg cgggaggaac gacatgaaag cggccgtact gtacggcaag ggagatctgc
     7561 gcatggagga tcggggcgtc cccgtcccgg gagccggcga ggtcctggtc aggatacggg
     7621 cctgcggagt ctgcgggacc gacgcggccc tcttccgggg ggaatacccg gcggccttcc
     7681 ccgtggtcat cggacacgag ttctccgggg aggtcgcggc cctcggcaag gacgtcggcg
     7741 gcttcaaggt cggagaccgg gtgaccgcgg acccgaacgt cctgtgcgga tcctgcggat
     7801 actgccgggg gggcctgggg cacctctgtt cccgggtaag ctccatgggg gtccatcggg
     7861 acggcgcgga cgccgaatac gccctggtcc ccgcggtcaa cctctacacc ttccccgaga
     7921 ccctcgcctt cgaggaggcg gccttcacgg agcccctggc ctgcgcggtg cacggagtcg
     7981 acctggccgg ggtgcgcaca ggtgacacgg tgctcgtcct gggagccggc gggatgggca
     8041 acctcatagc ccagctggcg cggctttccg gagccgcccg ggtggtggtt agcgagccca
     8101 tcgccatgcg cagggaccgg gccctggaga acggagccac ggacgcgatc gatcccgggg
     8161 ccggggacgt cggggaggct ctccggcgga tcaaccccct gggcgccgac gttgtcttcg
     8221 aggtggccgg ccacgtcggc ctccaggccc aggctgtccg gctggcccgc aaggggggga
     8281 cggtggtctg gttcggcgtg gctccgccgg acaaggtcgc cgaggtctcg cccttctacg
     8341 tcaacgagaa cgaaattcgg atcctcggat cctacaacaa tcccttcgcc accgaccggg
     8401 ccgtgaagct cctcgcggag cgccggatcc gggtggacaa cctcatcacc cacaggatag
     8461 ccctcgggga ctacccgaag gccctgtccc tgttcggcgg ccgggatacg gtcaagctca
     8521 tggtgacgat gggcgcatga tggaacggat cgtcgatatc gtcggcaacc tcaacatgga
     8581 cctgatcctt cgggggatcc ccgagatgcc ggcctggggc cgggaggtct tcgggaacga
     8641 ctgccttctg gtcccctcgg ggcaggccgg atacacggcc ctggccctcg gcgccctggg
     8701 ggtgcccgtc cgggtgcact cggtactcgg cggggacccc tggggcgatt ccatcctggc
     8761 cgccctggac ggttccggcg ccgacgttcg ggggatcgag cgtgccgcgg ggacccggac
     8821 gggcctatcc gtcgccctgg tgcgcggcga cggggaacgg gccttcgcct cgtactacgg
     8881 atccctggcg gctttcgacg cccaggccct ggggcggctc catccgggga tccgcgggcg
     8941 gggcgccctc ctggtctgcg ggatcaacgc cctgcccaac ctgcccctcg acgcggtcga
     9001 ggaaatctgc cgcgactacc gagggagcgg gggcctaacg gccctcgaca cgggctggga
     9061 tcccctgggc tgggggccgg aacgcctcgc gggcctcagg cgtctcctcc gggaaatctc
     9121 ggtgttcatc ccgaacgagg aagaggcccg ggcgatgacg gggttcgagg agcccgagga
     9181 gcaagccaag gccctcctcg gcttcggcgc cgggctcgtg gtcatcaagc tgggtccccg
     9241 cggctgctac gcggcaggcg gcggaagcgc ggaacgccta ccggcgtttc ccgccgaggt
     9301 cctcgacgcc gtgggcgcgg gggacgtttt caatgccgga ttcctggccg cgacgctctc
     9361 gggccgttcc ctgcgggagc ggatggccta cagctccgcg gcagcctcga tctatatttc
     9421 ccgggccgag aatcggtatc cgtcggcccg ggagaccgcg gccgtggcgg aatccctcgg
     9481 cgcgcccgga gctgtaaagg aaggcacatg atcgattttc acacacatcc cgtgatgatc
     9541 aaggaactcg tcgaggccga tccggatttc ggcagagcgg tacgggaggt cttcggcctg
     9601 ctgttcccgc cccagcccct cgagatcctg cgccgggaga tggacgccgc cggcattgag
     9661 agggccgtga tcctggccct cgacctaagc acggcccacg gctgccgggt gccggacaac
     9721 gagaccgtcg cccggctcgc ggagtcagaa ccccgcctca tcggcttcgc atccgtggac
     9781 cccaacctgc cggacgccgc ggagcgtctg gacggggcgg tgcggcggct cggcctccgg
     9841 gggctcaagc tcgacccgtc cctgcagcgc ttcgatcccg gagaccggag tagggcctac
     9901 cccctctacc gcctggccgg ggagctcggg atccccgtcc tgctccacgc cgggatgagc
     9961 tgggcgcccc ccggggtctc gcgcctttcc cggcccgtcc tgctcgagga ggccctgtcg
    10021 gaccatccgg gtactaattt cgtattggcc catttcggct ggccctggac ggacgaagcc
    10081 ctcatgctgg cgctgaagca caggaacctc tacctggaca cctcggtcgt ctacgcgagg
    10141 actcccgcgg agaccttacg gaaggtcgtg gcggagcaca tcggcctgcc ggtgctggag
    10201 ggcagcctcc cggacaggat cctcttcggc tcgaactacc cgcgccagga catgcgccgc
    10261 gccgcacggg gcatcgcctc cctgggcttc tccccgggac tcctcgaggc gatcacgcgg
    10321 ggcaacgccg cccggctcct cgggctcgaa acggaggcgc ggccatgatc agccacctca
    10381 aacggttcac gatcctcacc cggaaagaga cggagatcat cgacatcacc ccctccgtcc
    10441 tggagacggt cgccgcttcc ggagtctggt cgggcacggt cttcgtgctc tccctccaca
    10501 ccaccacggg cttctacgtg aacgagggcc tcccggacct ggagcgggac atggcggact
    10561 tcctggcccg cctggtcccc gaggatcatc cctaccggca cgcccggttc ctccactccg
    10621 acgggcagat ggccatcaac gccccttccc acctccgagg cacgatgctg ggtttccagg
    10681 ccttcttccc cgtcgaggaa ggcaagctcg tccgcggcgg caggcagacc atgtatctag
    10741 cggaactcga cggcccgcag gagcggacct atgtcgtcca ggttacgggc gaggcccggg
    10801 gaggtgaagc atgaccgagc ctaaatgggc ggagggcctg gaaggcgtga tcggcgccct
    10861 ggcggttccg atgttcgagg acggtagcat cgactactcc ctcctggaga agcaggccgc
    10921 ctactacggc ggatccggcg tccggggctt gttcctgggc gggacgaacg gcgaaggagc
    10981 ctacctgacc acggaggaga aggcccacat cctcaagacc gtccggggag ccgtcccgaa
    11041 ggacaagatc ctctgcgcgg cctgcatccg tccctctacc cgggaagtcc tggcggagat
    11101 gagggtcctg ggagctctcg ggccggacta catcgtcgcc gtggccccct actatctggc
    11161 agtcgaccag gaggccatca tccggcattt ccgcaccctc gcggcggcct ccccggctcc
    11221 cctcatcccg tacaacatcc cccagaacac ccacaacccc atgagcctga agaccatcca
    11281 ggagctcgcg tccattccga acatcgcggg gatcaaggat tcttcgggcg acttcatatc
    11341 cttcacccgc ggagtgctcg gtcccctgcc cggccccttc acttggatcc agggggagga
    11401 tcttctggac gccccctcct acctcatcgg cgccaaggcg gtggtgaccg ggctgtccaa
    11461 cgtctccgtg gaaccctatg tggacatgtg gagggccgcg acggccgggg acacggaggg
    11521 agtgaaggcc ggccagcggc ggataaacga gatgttcaag atcgtcctgg ccgtcggcgg
    11581 aaaaggcatc cccgccatca aagccgccat ggaactcctg ggccggggaa gtcgacatct
    11641 tcgactctcc gcgctcaccc tggacgacgc cgaagtagaa gccgtgcggg agacgctcgt
    11701 cgggatgggg ttgctgcgca ggtagcccgg gtctcccggc cgtcccacat cgcctagact
    11761 ggcaagagcg ccgcagccct tcagggttcc tggaacgtgc ggacaggagg tccgcaccga
    11821 cacccgcgga agcgccttgc gcttccgcga tggcgatgtt gtacannnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnggaag cgccttgcgc ttccgcgatg gcgatgttgt
    12001 acagggatgt acaacatcgc ctagactcgc aagagcgccg caggcccttt agggttcctg
    12061 gaacgtgcgg acaggaggtc cgcaccgaca cccgcggaag cgccttgcgc ttccgcgatg
    12121 gcgatgttgt acagggatgt acaacatcgc ctagactcgc ttccccttcc acaccccctt
    12181 cctgtaatgc caggccagga ccgcggcctc cacggaatcg gagaccagga agctcgccca
    12241 gatccccagg atcccgaggt ccagtccgag ccgcggaagc acgaaggccg acagggccag
    12301 gaaggggacc tgggcgcccc agcggccggc cagggaggag atcaggtagg ggatattgta
    12361 gcccgagccg gtgaaggcgc agcccaggcc gatggcgaag ctgacgatcg cgaaggaggg
    12421 acccaggaac cggatcatgg gcacgccgac cgcgaccgtc tcggcggagt ccacgaagaa
    12481 gcccaggacc gaccggggaa aggcgcaggc cagggccacg agggtggtga tcgtgatacc
    12541 cccgagcagg gcggccgcct tggcggtcct ctcggcacgg tcgacctgtt ccgcccccag
    12601 gttctggccg acgatggtgc cgccccctcc catcaagccg aagaggggca tgaacaccag
    12661 tcccgccaag cggttcccga tgcccagggc ggccagggcc gcggtcccgt accgggccac
    12721 gaacttggtg gtcaccaggc ccgcggcctg ccgggccagc atctcgccgc cgctcggtag
    12781 tcctatggtg atcagcttgc ggtcgatggc cggatccagc cggaagagcc cccggaccga
    12841 gagcttgatc cgagtgcgcc cgctggccag gagcccgaac ccgaccgcga aggccgcgca
    12901 gatgcagagc accgtcgcca gcccggcccc gaagaccccc agccccagtc ccctcacccc
    12961 gaaataggga acctcggaga acatgaacag gggatccagg accgcgttca ggatcgacac
    13021 tcccagcatg atccacatct ggctcttggg gtcccctgtg cagcgcaggg cggtgaaaca
    13081 gctgtaggtg gcgaagaaca cgggcaggaa gaaggtgcgg atacgcccgt actccagggc
    13141 gctccggatc acctcgggat ccgaggtgaa aaaacggatc aggggctcca gggcgaagta
    13201 caggaaggcc gaggcgatca cggccatgaa ggacttgaac acgatggtct gctcgatcac
    13261 ccgcgcggtc cgggccttgt ccccggcgcc gaaactctgg gtgatcaggg cgatggagga
    13321 cacgccgatc acctcgttga gcacctccac gagccagaag atggagctga acagggccac
    13381 tcccgccacg gcgctgccgg agatccgccc gatccagaac aggtcgacca cgtcgtacag
    13441 ggtctgtccg agcataccga gcatcgtggg cacggacatg gccaggaggt tttggatcac
    13501 cgaacctttt gtcaaatctc tagacatgga caccgttata gaaggagggc acggccgggg
    13561 tcaatcggga gggtccgcag gtcgggcgcg ctccggcccg aatcggcctt cctcagtcgg
    13621 agtccgaaac ggtctttgcc gcggtttccg cggacactgt cggccgagcc tcccgagcct
    13681 gccgctcgat cagcgcggcc accttcccca ggttggaagg ccgcaggatg tagtatccct
    13741 gcatggcccc gcacttcctg cgggcgaagt actgccactc ctcgcgggtc tcgatcccct
    13801 ccgcgatggt gtccagctgg aggttccgcg ccaggttcag gatggcgttc acgatcttct
    13861 ggttctgggg ctccgccagc tgggtcacga aggagatgtc gatcttgagg gcgtccacgg
    13921 gcagacggga aaggtaggcc agggaggaat accccgtgcc gaaatcgtcg atcatgaagc
    13981 ggatccccgg caggagccgc ttggcctcct tgatccgttc gatggcgtcc tgggtgttct
    14041 tcatgatcgc ggtctccgtc acctcgatgc acaggttgcc gggcggaagg ccggactgcc
    14101 ggacgatggc cgccagggtc ggcaccaggt tcggctggtc gaagtgccgt cccgagaggt
    14161 tgaccgacac gaagacgtcg gtgtgtcccc gggcatgcag cctccgggcg tcgtagcagg
    14221 ccttccggag agcccactcc cccagggcca tgacggtccc gttcgcctcc gccaggggga
    14281 tgaactccga ggggggcacc atccggccgt cgggtctgcg ccagcggagg agggcctcca
    14341 tcccccgcac ccggccgtcg ggtcccacga tgggctggta ggccaggctc aggcccccgg
    14401 aggtcatggc ggcggccagt tcctgctcca tcttccagtg ctccgtcgcc ttggcggtga
    14461 tgaccggatc gtacagggtg taccggttct tgccggcctc cttggagcag tacatggcga
    14521 tgtccgcgtt ccgggtggcc tcctccaggg tcgccccgta ctccgggatg acggcgatgc
    14581 cgatggagac cccgacgttc accttgcctc ctgcgttctt gcccttcgtc cggaggatgt
    14641 agggattgcc gacggtcgcc aggatgcgtt ccgcgacgat ggccgcgtcc ccggtctcct
    14701 tgatcccccc caggaccgct acgaactcgt cacccccgaa gcggtaggcg gaatcccggt
    14761 cccgcagaca gacccgcagg cgtcgacccg tctcgatcag gagctcgtcc ccggccgcat
    14821 gccccagggt gtcgttgacc cgtttgaagc cgtccaggtc caggaagagg acggcaccct
    14881 tccactcggg atcgtcccgg acccgatcca ggacggactt catgtcctcg ttgaacaggt
    14941 cccggttgcc caggcccgtc agcttgtcgt ggtaggccag gtggcggagc cggcgctcca
    15001 gggtcttccg ctgggtgatg tcccggatca cgcaggtcag gctccggccc ttgtagtcct
    15061 tggagttccc gaaggacatc tccatggggg cgacgccccg gtttttcgtg gcccccagga
    15121 tctccagggt gcgcttcatc ccttgggcca gccggtccgc gtcgtccacg aaaaagtact
    15181 tgttgaactc cttctcgtgc tgtgcgaaga cctccggagg gaagagcacc cggaagggct
    15241 tgcccaccac gtcctcccgc tcgaatccga aggtgttctt caccccggaa ttggcgaaga
    15301 tgatggagaa attctcgtcc agtcggaaga tggcctcggt aatggtttcc gacagggcca
    15361 cgtagtggtc cttggtctcc ttgagctgct cgaagagggc atggtcgaag gacaggtccc
    15421 ggataagcat caggaccgcg ggctggcccc cgcactggac cgaggagagg aggcagtcca
    15481 gggtcagggt gaccccgtcc ctgcgcaggc cgcggaaggc catgatgtcg tccgaggacg
    15541 cggagtgccg ctcaccctcc cggagaccga aggccgcgaa cttctgccgg aacacgtccc
    15601 ggtggctggg cggcaggagg gcgtagatct cggaatcgag gagctcctcc cgatcgtacc
    15661 cgaaggtccg atagaacgac gggttggcgt tgaggatggc tccggagggc gagacgatca
    15721 ggaccgagtc cgacaccccg tccaggagga tctcgaggct gtccgcctcg tcctcgcccg
    15781 attctatgcg cagggaatac ccctgcacga gccgttccag caactgggtg aagatggagt
    15841 cgtccggacg ggcgtcggag gcggcttcct ccggctcgag ggacttcagg atgctctgga
    15901 cctcgatgcc tttttccatg gatctaggct ctcctcattt tgacttaacc tcgggcatgc
    15961 gggtcgtgag ccgaattccc ccgcattcca ggctagtctc cggccgccgc cctgtcaagg
    16021 aaacccgggc cggaaggcct agaagatcca gaccagggcg gccaaacccg cgaaggtcat
    16081 ggccaaaaga cccttggcca aggcgatcct ggcctcgaag aagcgccgga tacgttcctg
    16141 ccgaaccccc aggagaatca ggcccaggac cgccaggagg ggcaggatga aggcgaggtt
    16201 gtatgtcagg aggcctccga tcccgagcca cccgccgccg gtctgggcca tgtaggacag
    16261 ggtcgggaaa tacacctggc ccgtgcaggc cagttccagg accgcgacga cgacgccggt
    16321 caggaagacc cccgccggaa gcacggcccc ccgcgtcccg ccccggatgg ccgcgtggat
    16381 gcctttccgt gccccttccg gaagtcccca cttcatgtcc gcggccttcc ccccccggag
    16441 caggaggagg tcccggacgg tcagggcgca gaacgcggcg gtcagccccg tcaccaggat
    16501 acgaagggcc agccggaatc ccgccatccg gatccccgac ctgagcaggt tgaacagacc
    16561 caggcccagc aggagatagg ccaggaagac ccccgcggcg aagaggagcc cgatgcccgc
    16621 gatccgtccc cgtcctgccc cgcccaggct cacgaaggac aggaagaaca agagggtcgt
    16681 gaacgcgcag gggttgatcc cgtccaccag tcccgccagg aggacgggca ggaactcgag
    16741 gcgcatcccc ggggacagag cccgttcccc ggacggatcc cgccgggggc ggaagccgcc
    16801 ctcccggacg aaatgttcca gttcgggaag aaggttctcc tcgatggccg cattgccctg
    16861 atatgcgttg ttcccgatca ctaggacggg gaagatccgg aattcctggc cgaactcggc
    16921 cagacgggcc cggcacctct cgtaccccga cgcggagagg atgtcgacct ggatcagctc
    16981 caccgccgcc ccggcccggg cggccgcggc cggaaggatc ttctcctcga aggtatcgca
    17041 gtgggagcac ccgatctccc cgtagtattc caggaccgcc gcaggctttt gtgaggccgc
    17101 ggcgccttgg aggaacaaca cggccagaca gcagagcgcc ccgttccgga tcgaaggaat
    17161 cccgttcgcc ttcatatcgt cctttcagta tcttataatg gatccatgag gcgaggtacc
    17221 gtcgcgggcc caaccccggc ggcctccttc gccgtatgct gagttataca aggaggactc
    17281 catgctcgtc caggaactca tgaccaagaa ccccctgacc acgacccccg atacctcggt
    17341 gccggacgcc ctggccctga tgcggcagaa aaaggtccgg cgcctgcccg tgctcgacaa
    17401 gaccggcaag ctggtcggca tcgtctcgga ccaggacctg ctctacgcct ccccgtcccc
    17461 ggccacctcc ctgagcgtgt tcgagatcaa ttccctgatc gccaagatca ccgtggccca
    17521 cgtcatgacc aagaaggtcg tgaccatcac ggaggacctg cccgtggagg aagccgcccg
    17581 catcatggcg gacaagaaga tcggcgggct tccggtcatg aagggaacca agctggcggg
    17641 catcgtgacc gagacggacc ttttccgggc cctgctccag ctcctgggtg gacggaggtc
    17701 cggagtccgc gtgacggtcc agattcccgg cgccaagggc accttcgcca agatctccgg
    17761 catcgtctcc gccgcggggg gcgacatcgt cgggctcggc gtcaccgagg cccagggcgt
    17821 ccccggagat ttctggcaca tgaccctcaa ggtccaggac ctgacgaaga aggccgtctc
    17881 cgcagccttc gccaaggccg gggtcgaggt cgtggacatc cgggagacta aagagtaggt
    17941 cttaggagac agcttcaggc aataaccacg gagcgcacgg agaaagatgg cctttttgcc
    18001 tctgtgtgct ccgtgttctc cgcggtgagt tccggggaaa acttttccgc ccttcctgga
    18061 aaaaggcaga gtcaccgttt atcgtctaac aggctggtga caaaggcgat ggcgcctttg
    18121 tcaccagcat ccttaatgtc gcaattgcgc agaaaagcgt aagcttttcg agcaattgca
    18181 tagcagggtg ctgacaagct tcgagtttgt cagcaccctg ctaactccgg gtgacccgtc
    18241 cgcaagcgtc gatggccagg agacacttgc aggcggggca gcggccccgc cccgctccgg
    18301 ggacccggag ggccttcccg cacacggggc aggccacccg taggggaaag acggccgcgc
    18361 ccgcgctccg gaagcctcgg gctgcctctt cccgggatgt accgatgttg aagtactcca
    18421 ggaatccgag gatccggaag acctcgagca ccttgggctg aacgttgtgg agatggatat
    18481 tcccgccccg gcttttgagc atcttcagga acgcggtgaa ggcacctatc ccccgggatg
    18541 agatgtaggt gacgttcccc aggtcgaaag ataggaagag gaaaccccgg tcgatgacct
    18601 tttccacccg cttggagaaa aacccgacgt tgaaaccgtc cagatagccg gacatccgga
    18661 tcacggctcc ccgttcgacc ccatccaggg gctccaggac gaggtccaag gtgtcgtcgg
    18721 tctcggaata gaagcccggc acgagctcga ggtcccggtt cacgacggct tcctcgtaac
    18781 ggcgacgggg gacagagctc cacgatttgg catgtttctc atgatgaaac agtatagtgt
    18841 attcctgcac gatatgcgaa tacttccgta ccgtcccggc ggctccggcc cgccctcgcg
    18901 tagccgtcct agccggcggc gggtatactc ctgtccatgt tccgcgaagt ctgcccggcc
    18961 tgccgccgcc ccctccccgt gtgcctctgc cccgacgaac ccccgatgga gacccggacc
    19021 cggatcgtcc tcctgaccca tcccaaggaa tggaaacgcc agagaagcgg aacgggacgg
    19081 ctgacgagcc tgcacctgcg gaactgcgag atcgtcccgg ggctccgctt cgacgagcac
    19141 ccccggatcc gatccctact ggaggacccg gagggctatc cggtcctgct gtatcccggt
    19201 cccggggcct ggaacctgtc ggagcgggag ttcccggcga aggcgctgga agggcgccgc
    19261 ctaacggttt tcctggtgga cgcgacctgg gcctgctccc gggcggttct ccgggaaagc
    19321 ccgggcctga gccgcctccc ccgggtcatg ttcacacccc gggagcccag ccgctgggtt
    19381 atcaagcgcc agccccggcc ggagtgcctg tccaccctgg aggccgtgca cgagcttctg
    19441 accgccctgg acgccgcggg cctggacgag tatcccgacc ggacacgcct gctggaggcc
    19501 ttccgacgca tgcaggagat ccaggtcgaa tgcgcccgga cccgcgggcg tgcccggttc
    19561 cggggctacg accgcgtcga caggctccta gaagcggagg gagaggcagg tgagcccgcc
    19621 gtcgatcttc cggaactccg aggtgtccac cgataggacg gggtagccca ggtcttccaa
    19681 ggccgtccgg atcccgggga agcccgcggg gacgaggacg ttcccgttca cccagaggca
    19741 gttcgcggcg taggcctccg cctcgggcac cgggatcttc tcgaagtccc ggaacggggg
    19801 atactcggcg aactcctccg tgaccaggag ccggccgcct tccaggaaac tcaagcccgt
    19861 cttcaggtga aggaccccgg tcacgggcac cggctggccc gggatgcccc agtcccggag
    19921 caggttgagg aactggatcg ctccctcttg gttcgtccgg tccgagagcc ccacgaagac
    19981 ctccccgccc gcccggagga tgtccccgcc ctccagggtt ccgggggccc ggatgcggaa
    20041 gatccggtcc ggggtataga aggatcgcag ggcgcgctcc atgtcctcga cctccccttt
    20101 tctcgaagag gcgccggggt tggtcagcac cgcccgatcg ggcaggcaga acgccgtgtc
    20161 ctcgacgaag caggagtccg ggtattcctc cagggcttcc agcaccgtca cggacaggcc
    20221 gcaggcctcc agggcccgga tgtagtcatc gtgctgtcgc agggccttct cgtaatccgg
    20281 agtgcctaag tggggggagg aagtgagtcc cgagaccagg gatcgggcgg gtcttcggac
    20341 gacggcgtat cggaacatgg caacctccta ggagtttgtc gggcttgccc aataaaacca
    20401 aagaaaaatc gcatacgtgc gatttttcta cctcgcaact cccgaggagc ccgacaatcg
    20461 ggacttttta cgccttcagg cgtaaaaagt ccacaagatc gacaggctcc tagaacgggt
    20521 ccttcccggc cggatccagg actccggcca ccgcctcggc cagcaggacc ggggtgcgct
    20581 cggtcaggtc ctgccgggac accctctccg tccttttgtg gaaatccttg ccccaggggc
    20641 cgatgttcac acagggcatc gagatcccgg cgatccgccc gaaggggatg gaataggcgt
    20701 cgccgtaaag gggcatctcg tcgcggatca gggtctcgat tccctcggcg tccaggatgg
    20761 aagagtagct caggtcggag atccccgtga agtaggactc cagctcgtat tcctggcccc
    20821 agagctcccg ggccctaccg gaaagacggg ccgccaggga acggatcgcc gcgtcgaagt
    20881 ccggccgatc ccgatgggac acgctgggat agtagggagg caggaatccc gtcacgacca
    20941 ggggccggcc cccgcccagg ccgcccgcca gggcgtccac gagggaccag accgcccgga
    21001 agaggggttc cccccgccgg actcgttccg cggccgcttc ccgggtcttc tcgaaaaggg
    21061 cttcatcctt ggaccggagc tcccgggcga gacttccgaa ggagaggacc cggggcttcc
    21121 ggtcgccggt ccaggccggg ctttcctcga cggcccggcc cgtccgggag cggaagagcc
    21181 gggaggatcg caccgcggtt tcggaggcct cccccgccgc ggcctcgcag atccggagca
    21241 gggactcccg gacgcggccg gggcggtttt ccaggggctg gacgctcagg attccgaagg
    21301 cggacagggg catggagacg tcgtaagcct ccttgctgtc ccgggccatg agccaggtcg
    21361 ggggaggcga catctcgggt tccggttcca ggcaatccgc cagctccgtc gacatctccg
    21421 tcccgcgcac gatccgggac aggatgccca gggggttgaa gccctccgcg ctcttccccg
    21481 cgtgggccag gatgccccgg acgtacacga agggcaggat cttcccgatg gacccgccgg
    21541 agaggatccc cgtgtccgcg ttcttccttt ggtggggctc ggagttgatc atgaggacgt
    21601 agtccaggcc gtaccggccc ttgaggtccg caaggaggtc cacggcggcc cgcatcccgg
    21661 cggagaggtt ctcctcgtcc ggcagggcga tcaaaaggag gtttccctta aaatacgggt
    21721 cccgggcggc ctcgtccagc agggccatct ggatggcccc tccggccttc atgtccgcgg
    21781 ccccgcggcc gaagatgaat cccccgctct ccaggtcggc gcggctctcg tcgtccaggg
    21841 acccgggatt ttcccggagg gccgccgcga gccgttccgg gtcgaaggcc agatgcccca
    21901 gggggccgta gtcctcgatc tccaccacgt cgaagtggtg catgaggacc gcggtccgtt
    21961 tcccccggcc gcggaccagg gcccactcca cggtccgccc ggccgggtcc gccgggacgg
    22021 gatgggtccc gaagaatccc gggccgccgg cgaaccgcgg cgacttctcg aagtgggccc
    22081 ggaagaaggc tgccgcgagg ttttcccggg ctgtcccgga ctcggaattg acggccacgt
    22141 agggatagaa gatttccagg atttcccggg ccgaaggtgg attcacgcga cgctcctatc
    22201 accctaaaaa gcaaaaagcc gtccggagga cggctcgctg ccatttcgtg gaggtgaggg
    22261 gaatcggcct tcgggcctcg atccgatctc ggcccttcgg cccggggttc gccctggcgc
    22321 gcgcggacgt cgcttcgctc cgtctaccga taggaaacga ttcacgtgcg cgctatcgcg
    22381 cgccccggta tccctgcgcg cttccgccgc ttccgcggcg cgggcgaacc cttcaattcc
    22441 cctctcgttc catgaaaaaa cgacgagccg tccggaggac ggctcgctgc catttcgtgg
    22501 aggtgagggg aattgaaccc ctgacctcat gaatgccatt catgcgctct agccaactga
    22561 gctacacccc cgaaacgtgg gggcagtata cggatagaag ggccgagtgt caacgggagg
    22621 gccgaaggcc gccgtcttcg aggccgccgg gccggcccgg ccgtattgcg ctcgggacgg
    22681 tccgcggtcc atactccagt catgccgaag tccaacgaag cgtacgccgc tatctgcccc
    22741 gtgggcctgg aaaaggtcct ggcccaggaa ctctcccgga tgggaatcga ggccgaatcc
    22801 cgcgatcccg gccgggtagg cttccgggcg gacgcccggg gcgtcgcctc ggccctgtgc
    22861 gcgcttcgca ccgccgatcg catcctggcg gccccgggcg ggttccacgc cccggatttc
    22921 gactccctct tcgagggcat gcgggccctg ccctgggagc gctggatcgg ccgggaagac
    22981 cgggtcacca tcgtccgggt ccgttccagg gcgtccgccc ttggggccca aacctccgtg
    23041 caggcggtag cccacaaggc cgtctacgag cggctcggcc gtgtatacaa ggtgtcccgc
    23101 atgccccaga ccggaccgga acgggaggtg agactctacc tggacgacga cctgtgcacg
    23161 cccttgttgg acctttccgg agaccccctg tacaagcggg gttggcgcac ggagtccgtg
    23221 gaagccccgc tccgggagac cgtggccgcg ggccttctgt tcctttcggg ctggacgcga
    23281 aaacgtcccc tccgggaccc cttctgcgga gggggaaccc tggccgtgga ggccgccctc
    23341 tgggcccggg acgaggcccc caatctccgt cggcgcttcg cctgggagac catgcccagc
    23401 ttcgaccccg aggctctcca gggagtccgg gcggacctgg aaggccgaat ccggcgggac
    23461 atccgggcgg acatcctggg gagcgaccgc gacgaaggcg ccgtgtccgt cgcccgggcg
    23521 aacgcccgaa gggccggggt cgaggacctg gtccggttcg agcggctgga catggaggac
    23581 tcatcctccg gaggtcttcc ggagggcctg gtgctggcca acccgcccta cgggctccgc
    23641 atggggaccg aggcggaggc ggaggtcctc tggcgcgcgg ccggaggcct gaaggaccgc
    23701 tacccgggct ggagcttcgg gttcatcgcg aaccgggagg atttcccggc gttcttcgga
    23761 gcccggcccg cgtccagccg gacgatcctg gcgggggcgg agaaactctg gttccattgg
    23821 ttccccgccg cgtccggatc cggcaagggt tgacgaaaaa aaccttgcgc ccacgggcgg
    23881 taccgtagta caatacctcc ggccgggtcc aatcgatcgt tttctaccca aacacccggt
    23941 accaatcgcc acaattgtca gggaggaaga ttcatgaagc gttccatcgc catcctcggt
    24001 atcgctgtcc ttctggtcgc ggcccttctc atcggctgcc agcccaagga agtgaaaccc
    24061 acgttcatca acatcgccac cggcggtacc gccggcacct atttccccct cggtggagcc
    24121 ttggccgaga tctggaacac caacatcaag ggcatgaacg ccaccgccca gagcaccggc
    24181 gcctcggtcg ccaacatcaa cctcctgcgg gacggcaaga acgaagtcat catggtccag
    24241 aacgacatct gctactacgc cgtgaacggc ctggagatgt tcaaggacaa ggccttcgcg
    24301 gacatccgcg gcctggtgac cctttataac gaaacctgcc aagtcgtcac cctggccgac
    24361 aagggcatca acggcatcgc cgacctcaag ggcaagcgcg tggccgtggg cgcggccggt
    24421 tccggagccg aggccaacgc ccgccagatc ctcgaggccg ccgggctttc ctacagcgac
    24481 ctgaccgccc agtacctctc cttcgcggaa gccgccaaca acctcaagga cggaaacatc
    24541 gacgccgcct tcgtgaccgc cggccacccg accgcggcca tccaggacat cgccgccagc
    24601 cgcaaggtcg ccctggtcaa catctccccc gagatcatca ccgccatcac ctccaagtat
    24661 ccctactaca cccccaccac gatccccgcg ggaacctaca ccggcgtgga cgttccggtc
    24721 agcaccgtcg ccgtgaaggc catgctcgcg gtcaaggccg acctggacgc caaggtcgcc
    24781 tacgagatgg tcaagaccat gtacgccaac accgagcgcc tcaaggccgc ccacaaggtc
    24841 ggttcccaga tcaccgtgga caccggcaag gcgggcatgc ccctgaccct gcaccccgga
    24901 gccgagaagt tcttcaagga agcgggcaag taagcacccg cgcaccggat cgaggctcgg
    24961 cctcgatccg gccgcccatg accttacggg gtgtcccgtg cggctcgtcc gcctggacac
    25021 cccctttttt tcgggatcga tgcggcgccc cattcgtttg ttcgcccttg cagccgtcct
    25081 ggtactcatc gccggttacg ccctgctgcc caggacgccg accttgacga tccggaacga
    25141 acgcgggagg atcctggcgc ggctcgcctg cgaggatgga aggttcgtgc accgttacgt
    25201 ccattcggtg cacctttccg acgtggacga ggagtattcg atcgagtcgg acgggagcct
    25261 aaggcttacc gccacccggt tcgacacctt gggggtcggg ataccctacg acgccgaggg
    25321 cggattctcc aaggacggaa accgcttcgt cctgcggatg gaccgtcggt accggtccct
    25381 gcccgtccgg gtgtccccga tcccgggaca tgtgatcgtg acggggggcc ggatcttccc
    25441 gctctccgag tgggtcggcc cgggagaact ggccgtcctg gaagcccgaa acatcccccg
    25501 caccgggatc ctaactcccc ggcgggaaga ccgataacgc atagccatcc ggaggattgg
    25561 gcatgagcga acgcaagaaa aaagaacctg ccgcggcagg tgagagcacg ccgaaaaaac
    25621 gggccgcctc gtcgaagaaa accgcatccg gtacgcaagt accggcggcg tccgtggaag
    25681 cagcggacct ctcggacacg gtgaacgggg ccgagatcct caagaaattc gacaaggagt
    25741 cggacttccg caccctggcc tgtttcctgg gcaagttcat cgcggccctg gccatcgcgt
    25801 tctcggtgtt ccaggtgtac accgccgcct tcggcgtcct ggacgccatg atccagcggg
    25861 ccatccacct gggcttcgga cttaccctga tctacctgct gtacccgacc agcaagaagt
    25921 ggccccgcga caagctgcac ccggtggacg cgttactggc cgtggcgtcc gcggtcgctc
    25981 cgatgtacat cctgatcacc taccagcagc tcgtcctccg ggccggaacc acgaccccga
    26041 tggatatcgc gatgggactg ctcggcatca tcctggtgtt cgaggcggcg cggcgggtcg
    26101 tgggccttcc catcgtgatc atcgccggga ttttcgtcct gtacgccttc gcgggcccct
    26161 acatcccggg ccgcctggcg caccggggcg cccggctcga taccctggtc ggccacctct
    26221 tcttcaccac cgagggcatc ttcggcatcc ccctgggggt gtcgagcacc ttcatcttcc
    26281 tgttcatcct cttcggcgct tacctggaga agaccgggct gggccagtac ttcatcgaca
    26341 tcgccaactc catcgcgggc tgggcgtccg ggggacccgc caaggtggcc gtgctctcca
    26401 gcgccctcat gggcaccgtt tccggaagct ccgtggccaa cgtggtcggc accggcagtt
    26461 tcaccatccc gatgatgaag aagctggggt acaagcccga gttcgccggg gccgtagaag
    26521 ccacggccag cacgggcggc cagatcatgc ctcccatcat gggcgccgcg gccttcctga
    26581 tggcggagtt cacgggcatc ccctacgccc ggatcatcgg cgccgccgtt gtgccggcgc
    26641 tcctgtacta cttcggggtc tgggccgggg tccacatcga ggccaagcgg ctcgggctcc
    26701 ggggcctgcg aaaggatgaa ctgccgaaac tcggagaact cctgaagaag tccttcctga
    26761 tgcttcccct cgccgcgatc atctacctct tggtgtccgg atacaccccc atgcgggccg
    26821 ccctgatcgc catcgtgctg tccatcggaa ccgcgggcgc cgcatccctg gtcaactggt
    26881 ccaggaagcg gcccgtcggt ttccgttggg tggacatcgt gaaggggctg gaagcgggag
    26941 cccggggtgc cctgggtgtc ctggcagcca cggcctgcgc gggcatcatc atcggcgtgg
    27001 tcaccctcac cggtctcggc ctgaaactgg gctccagcct ggtggccctg gcgaacggcc
    27061 agctcttcct gaccctggtc ttcacgatgt tcacgtccct gatcctgggc atgggagtcc
    27121 cgaccacggc caactacgtg atcacctcca cgatagccgc accggccatc atgatgatcc
    27181 tgacgaaggt caatcccggg atggatccct acgcccaggc gatcgtcctg cccgcccaca
    27241 tgttcgcctt ctacttcggc atcatcgcgg acgtaacccc gccggtcgcc ctggcggcct
    27301 tcgcgggggc gggaatagcc aaggccaatc cgatgaagac ggggttcaac gccaccaagc
    27361 tggccatcgc ggccttcctg gtgccctaca tctttgtgta tagcccccag atgctcggca
    27421 tcggtgccac cttcctggac atggtcggga tcttcgttac ttcctcaatc gggatgatcg
    27481 gagtgtccgc gggggtcatg ggatacctca tccgtcccat gcacctgtgg gagaggccca
    27541 tcctcttcat cggcggaatc ctgatggtga tcccgggggc cgtgaccgat gccataggcc
    27601 tggc
//
LOCUS       DAOTEI010000027        27180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1152_length_27180_cov_4.865106, whole genome shotgun sequence.
ACCESSION   DAOTEI010000027 DAOTEI010000000
VERSION     DAOTEI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 27180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27180
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1152_length_27180_cov_4.865106"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="P5313_04260"
     CDS             <1..547
                     /locus_tag="P5313_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HRY79611.1"
                     /translation="QQAIGASLGEDSIEEGKRAVLIGLLAVMAFMLVYYKGAGINAAL
                     AQVVNLYLMLSILSGFNLTLTLPAIAGFVLTVGMSVDANVIIFERIKEELLAGKGRRA
                     AVEAGFGKAFWAVMDGNITTIIAALFLAQLGSGPIQGFAVSLAIGNLSSLFTALFVSR
                     LLFDFGTDRGLKGVSISWRIK"
     gene            544..1806
                     /gene="secF"
                     /locus_tag="P5313_04265"
     CDS             544..1806
                     /gene="secF"
                     /locus_tag="P5313_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRY79612.1"
                     /translation="MKRVIRFDRGFLAAVLLSVALIAFGIVGLATKGINLGVDFQAGI
                     NQYVQLVYPALDVSYSGTGNAVLSVSDTRAVLVFSGADVENRTIEYDLAASGTLSDLA
                     SAFSSQPGVSARVTDGAGKPAVTLVPTYQGDIRLAAAAVRLSREPETETEYFAGMDKV
                     RAAAATIGSVSVQSVGERIRQQYIIRLRDDGTDPKFSTTSAERIRTALETAFGAGRVV
                     VMKTDFVGARFSKDLADNAWKLTLFTVLAILAYATVRFKIQYAMGAVLAILHDALIMI
                     AFIVWTRIEFNTSTLAAILTILGYSINDTIVIFDRIREDRRLLPTEPFHTVLNKSITE
                     TLGRTVITTITTMLAVLSIFFFTSGSIKDFALTLFVGMVSGTYSTIFIATAFVAFWND
                     QAEKRKKAQGTAPTAKKPGTAPVLKPAK"
     gene            2058..2129
                     /locus_tag="P5313_04270"
     tRNA            2058..2129
                     /locus_tag="P5313_04270"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2090..2092,aa:Cys,seq:gca)
     gene            complement(2537..2797)
                     /locus_tag="P5313_04275"
     CDS             complement(2537..2797)
                     /locus_tag="P5313_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79613.1"
                     /translation="MPQGLPPDRAPNCLACRHFIVTWEPAYPRGCEVFGIKSRNLPSA
                     EVFAATGLHCPAFEAIEPMERREGPRPGGSGDAGSENGGIWA"
     gene            2859..3497
                     /locus_tag="P5313_04280"
     CDS             2859..3497
                     /locus_tag="P5313_04280"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY79614.1"
                     /translation="MNSRSVLRRPFRYQFHNATLVLIAVNVGVFFLTQISPDFLVNLA
                     MVPAYVVRGRFWLLFTYMFAHGGISHLFFNMLGLFFFGTHVERELGSCEFLLFYLLTG
                     VLAGAFSFAAYAATGAWITFLIGASGAIFAVLLAFAALFPGSIIHVWGILPVRAPVLV
                     LAYTAIEIASQLFSFRSGVAHLTHLAGFGFAYLYLLVRLDINAFRRFFPRDR"
     gene            3551..4312
                     /locus_tag="P5313_04285"
     CDS             3551..4312
                     /locus_tag="P5313_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRY79615.1"
                     /translation="MNLPELDAWCRSFLEIEAFKKVDDSLNGIQVGRSEGPVTRVAFA
                     VDACAETIRRAAEEKAQVLFVHHGLFWGSPARIQGALMERVRTLLDRDMALYACHLPL
                     DAHPEYGNNAVLARMLGLSGVKPFGFYKGISLGFRGVLDPAPTLEEAIRRILPDGSKP
                     RAVLPFGPDRISSAAVVSGGAPHELFEAVEEGLDLYVTGEPSHSVYHAAQESGINFVA
                     AGHYATEVWGVRAVAEKLARETGTETVFVEVPTGL"
     gene            4336..4881
                     /gene="lspA"
                     /locus_tag="P5313_04290"
     CDS             4336..4881
                     /gene="lspA"
                     /locus_tag="P5313_04290"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRY79616.1"
                     /translation="MDSDKPNSLRPFLLTAGILIADQVSKALIVDLIPRNTIAVRGLG
                     DFLWIVHARNTGIAFSMGDGLPPWMRMVLFSLLPLALIAGILIYYFRTDETTPVQRWA
                     LAAIVGGGLGNVVDRLFRPEGVVDFVSVKFYGLLGMERWPTFNVADSAVVLGALLVAA
                     TSLLAPGPGTPVRDKDGGSAG"
     gene            4878..5180
                     /locus_tag="P5313_04295"
     CDS             4878..5180
                     /locus_tag="P5313_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79617.1"
                     /translation="MRDPKGDPVPAARTPGARARGILLFLAATILNFLTASAFFFGFL
                     ALWGLVLAPWLKLPTSSPVILAAFVLAVAGSGILYRAAVKIYLRKTGRAPGHLTKS"
     gene            5232..6569
                     /locus_tag="P5313_04300"
     CDS             5232..6569
                     /locus_tag="P5313_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRY79618.1"
                     /translation="MASFEDKLALAGVRVPEVRLPARGADWEAWAVVACDQYSSEREY
                     WEGVKDRIGGKPSTLNLIFPECYLEDSDKDERILRIQAAMRDYLDRGVLESAGEGFIL
                     VERRTPFEAEPRKGLMIAVDLEKYEYGKGSASLIRPTEGTIVERLPPRMKIRRGAPLE
                     LPHIMLLIDDPGRTVIEPVYEARETLKPIYDFELMTGSGHVRGWQVRGEAALGGIADA
                     LTRLADPAAYKRKYGKDGVLLFAVGDGNHSLATAKAVWEEIKGANAGKPGIERHPARW
                     ALVELVNIYDEGLPFHPIHRVLFQADEKTFLAELAAHGDCRVEEASKAAAFAAVDEGA
                     AEHRFAILTAGGTRLVRFPRPKANLAAGTIQEFLDGYLARHPGTGIDYIHGRESLETL
                     AAKPGNLGIYLPPIDKAGFFGTVIRDGVMPRKTFSMGEAPEKRFYIEARKILP"
     gene            6744..7616
                     /locus_tag="P5313_04305"
     CDS             6744..7616
                     /locus_tag="P5313_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRY79619.1"
                     /translation="MVILLADAFLPDLPKRLAAFGEVTQDMGRVGEAEVMIVRSKTKV
                     GKDVIDKAPKLKFVIRGGVGVDTIDVPYAESKKIKVTNTPEASSVAVAELAFSMMLAL
                     ASRICEADASMKQGKWLKKDLERTELMGKTLGLVGIGRIAREVALRAKAFGMEVIAFD
                     KFVPKSDVAAMVSLEEVWAKSDYISLHVPLTDETKGMINAAAIAKMKKGVRVVNTCRG
                     QVVSEEDVAAALADGRLGGYGADVFYSEPPEGSPLLSAPRTVLAPHLGASTEENLARL
                     GDCIVDRMKEYVKK"
     gene            7713..8804
                     /gene="serC"
                     /locus_tag="P5313_04310"
     CDS             7713..8804
                     /gene="serC"
                     /locus_tag="P5313_04310"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRY79620.1"
                     /translation="MKRVINYSAGPAAIPVEVLEQARDEMLDWNGTGCSVMEVSHRGK
                     EYDALHMETMDLFREIAGLGKNYEVLFCTGGASTQFLMLPMNYLGPGKEADYVVTGNW
                     SKASIKEAKRFGKITFPANMELEGERFVAIPKQGDLKLNPAAEYVHYTSNNTVEGTEW
                     HYIPDVKGKPLVCDMSSDIFSRPLDFNKFSLIYAGAQKNLGPSGVTVVAVRDDFLAKA
                     HDDLPTMMSYKVFKDKFSMHNTPPCFSIYILNLVLKWLKKNGGLAAMGKTNERKQKLL
                     YAAVDGSKGFYKGLAEVDSRSWMNIDFKLPTPELDDKFVKEAKAAGIVQTKGYRSMGG
                     IRLSAYNAVSLKDVETTVAFMQDFMAKNG"
     gene            complement(8983..11187)
                     /locus_tag="P5313_04315"
     CDS             complement(8983..11187)
                     /locus_tag="P5313_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HRY79621.1"
                     /translation="MNDITLSDETGYGRSFIPSGFLPPGKDEYHLRNSTLGKPLDSYR
                     PLRAAEIETLARNGNTCTDWSGILVRDPFIPDLIRGCDFAGLVRIGRLERVVLSHHDL
                     ETPAGITDSRIVACDIGDLCAIHDCDYIAHYQIGDSCILLNNNEIHATNHAKFGNGVV
                     MEGEEESVRVWIDLMNEAGGRSVLPFDGMICADAWLWAKRRDDSALMERFKALTQAGF
                     ENRRGGYGRIGAGSVLKHNRIIKDARIGDCAYIKGANKLKNITINSSEAERTQIGEGV
                     ELVNGIVGYGCRAFYGVKAIRFIMGNNSALKYGARLIHSVLGDNSTVSCCELLNNLIF
                     PAHEQHHNTSFLIASVVQGQSNLAAGCTIGSNHNSRANDGEIQAARGFWPGLSTSLKH
                     SCRFASYVLLSKGDYPAELDIPLPFCLVNENPAKDRIELIPAYWWMYNLYALLRNESK
                     FAARDRRITKVQALELSPLAPDTAEEILRARVLLEEWAGTAAVLAEGGDPSAPGPEAL
                     RERGRALLAGPEDGKVAVHAYGVENSTRPVLILKAGRAWKAYRRMLHWYAVREILAYF
                     EARPDMTVSDAAEALDGPREREWENLGGQIVPARKVEALIAEIRSGVLGSWDEIHAAY
                     GRLASEYPVDKARHAWACLRDLEGWTSIGPRELGKALDLLEEGSRYVEEQVYLSRAKD
                     YSNPFRRATFRNPEELRAVCGSPEGNPFVRATKEDAERLRGRIDALRARIRK"
     gene            11273..11848
                     /locus_tag="P5313_04320"
     CDS             11273..11848
                     /locus_tag="P5313_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79622.1"
                     /translation="MSVPEILAEDPIAVDVEKAAYEIFPLLVQAENRVLREQYATAFA
                     NLLGNPGEFYTIVSGSMGERLVHVERLLHAFRENVELLIRKTWVEKHDEKSKEKVHED
                     LSVFVQEFQSGAVRKAFPHFVSLSLGIARLLFGAQAQAPDFLEYTFRIDPKLGVFYWF
                     IGRLKEQESAEVAEDLRTLELLLGVFALASF"
     gene            11854..12915
                     /gene="mtnA"
                     /locus_tag="P5313_04325"
     CDS             11854..12915
                     /gene="mtnA"
                     /locus_tag="P5313_04325"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRY79623.1"
                     /translation="MPDAGSLRSVRLDEEAGELELIDQTLLPNRTVLLRLSDPRDIRE
                     AIYSLRVRGAPAIGIAAAYGVYLAIRRSAARDWDALAVEFRAAKGLLASARPTAVNLS
                     WALDRMERRLDAARGRPVGEVKNALLEEAEAVRAEDEEACRAIGENGLSLLGPGWGIL
                     THCNAGWLATSRYGTALAPLYLGAERGYGFRVFADETRPLLQGARLTAWELDRAGIDL
                     TLICDNMASTVMKRGLVKAVLTGCDRVAANGDVANKIGTSGVAVLARHYGIPFYVLGP
                     VSTVDLACPTGAEIPVEERPAEEVTDRWYVRRMAPEGVKVLNPAFDVTDAGLITAIVT
                     ERGIARPPYGDSLARIVQP"
     gene            complement(12918..14957)
                     /gene="fusA"
                     /locus_tag="P5313_04330"
     CDS             complement(12918..14957)
                     /gene="fusA"
                     /locus_tag="P5313_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY79624.1"
                     /translation="MDLDTTRNIGIMAHIDAGKTTTTERILYYSGKTYRLGEVDDGEA
                     TMDWMEQEQERGITITSAATTTFWRNHQINIIDTPGHVDFTAEVERSLRVLDGAVAVF
                     CAVGGVEPQSETVWHQADRYHVPRIAYVNKMDRLGADFFAVLEDMRTKLGANPVPVAI
                     PIGREGAFEGMVDLLAREELRWDAAEGGQHITRTPLSPERATEADAWREKLMDSLGTW
                     SDEIAELFLAGGEVPPELLRREIRKQTLARNIVPVLCGASRRNIGVQALLDAVVDYLP
                     SPHEVPAARAFHLKKEEDIEVPCESAGNPLGLVFKVQYDREAGPLCFVRMYSGSVKNA
                     STVYNIGKKKRERVTRLLRMHANKSEPLDEVGAGDIAVIVGMKAAQTGDTIGSEGWPV
                     VLERMHFPEPVISVAIEPKTLSDRDKLKSTLDLLSLEDPTFTSKENDETGQLIIQGMG
                     ELHLDVLVTRIIRDYKVDARVGNPQVTYRESVTKAVEHTEAFRKVLAGKENAARLTLR
                     VEPRARGEGNLYRKDIRNPKVPDEIWEAVETAVTNAFAGGIQFGYPCVDVGVTLTDLE
                     WDELTASPYAFQAAAALGFDEACRKAAPVLLEPIMKVDIVTPSDFLGEAMSLVSQRGG
                     MIHGTDSKAASDTIHAEAPLSVMFGFTTALRSVTQGRAGFSMEFSHFQPKRNQGA"
     gene            15087..17498
                     /locus_tag="P5313_04335"
     CDS             15087..17498
                     /locus_tag="P5313_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRY79625.1"
                     /translation="MPSSLELLAPAGNPEALDAAVGEGADAVYLGLRSFNARMRSANF
                     AYNQFEAAVEVLHKQSKRVYVTVNTVFEEREADRMYQFLQYLDKVGPDGIIVQDFGVV
                     KMARDHFPSLRLHASTQMNVAGAAGANQLSRWGFKRVVLARELSLDEIAAVRRSTNLE
                     LETFVHGALCVSVSGLCLFSSWLGGKSANRGGCAQACRRLYSNDRETGYYFSPDDLEL
                     IARVPDLAEAGVSALKIEGRMKSSEYVGTVVGAYRYMIDNYRLDPERALAKAQGMLRN
                     DFARRKTTYWFEGTAAPDFIRPDQAGGTGIPLGKVREVRAFEGKLEGRWALLNTFEGI
                     AEGDSVRVHRADDSGRETGKVKEVLQKAEGMYLRLELPFRQNDQVYLIQTRSMARRYK
                     SVLPSSLERFRRFPSYDSAPRVEFPKLPAERLDSFPAGIYALVGRTADLHVLLASRPV
                     KAMLRLNRRTAADLRASESGAPFKKDSLVLWLDPFFPEGDAAWLAEELDYWISRGQRF
                     FVVNNLGHLNLLKNRGVVLAAGPWLYAFNVRAAAFLFENGVDCLIPPHEISKQALQQI
                     AEVIPPRAIMPLAFSFPPLFTIRSDLASEYKFRNFSDKEGTAYELVRDGTGSAVIPSR
                     PLSLTDRVPFLQKDGYEKFLLDFGHMELAKPMYKQVFQAAREGIVLPETGRFNWKSGF
                     WNPDEGSPARDDSKGGPGRRTAGAGTRSGGPGRSATAGRVGRSAGRDASDRPDAAGKP
                     GTRRPRSGPESGTSDRKPARGGRRPAGRGPSAGPGTGSGSGPSRDRKPRTGPKRPGAG
                     RKSRD"
     gene            17681..18445
                     /locus_tag="P5313_04340"
     CDS             17681..18445
                     /locus_tag="P5313_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRY79626.1"
                     /translation="MSGSTVKAFELLTYLSRAGEAVTLARISRETGWDKATALRYLGS
                     LAEAGAVEKRENGWVLGLALIELASRVPVSENVAARARAVLERLARETGETINLGSWS
                     NGGAYYLAKADGGRNLRLRTSPGDRLPLHCTAVGKAILATLDSAYLEDFLQQAPYPRL
                     TPTTITEAQAFREEIGQVRNVGYAMDREEYEPGLVCIAVPMEIPALGFLGAVSVSGPS
                     VRMRDPFGSILDLLRAASRDIVAAVTAAQTGEAGED"
     gene            complement(18628..19026)
                     /locus_tag="P5313_04345"
     CDS             complement(18628..19026)
                     /locus_tag="P5313_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRY79627.1"
                     /translation="MNNLNSILLEGNLVRDPDLKATPSGTPVCTFSVASNRWYKQNDQ
                     LEKEVSFFDVEAWSKLAQTCGDTLKKGRGVRVVGRLKQDRWNDGEGKSHNKVKIVAEH
                     VEFKPQMKDDASGERPPSSRARKEEPAEVF"
     gene            19259..19975
                     /locus_tag="P5313_04350"
     CDS             19259..19975
                     /locus_tag="P5313_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRY79628.1"
                     /translation="MDPSRPGRARPLSAALRIAVFAATLASCAGRGGSPSAKDAAYPF
                     PERETYAPAVPTKLQTAVVSGGLSLVGRSELVVNGTRYPMDCTGVVRAAYAFGGMDLA
                     YRFAFYPGNGVARLYGTLRDAGLLYSTRYPAPGDLVFWDDTYDANGNGRADDPLTHVG
                     MVVEVDEEGTVSYLHHNYRRGPVVERMNLIHPGVETLFVKGVSVTVNSPLRMRGSPPG
                     PGTTAGELFRVFGAAYKLSR"
     gene            20079..20645
                     /locus_tag="P5313_04355"
     CDS             20079..20645
                     /locus_tag="P5313_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRY79629.1"
                     /translation="MSIQHAILGFLSWKPHTGYELKKVFADSLSFHWSGNNNQIYGTL
                     IKLHQEGLVTNEVVYQDKYPARKVYTVTPKGLETLRAWLGSEPELPELRNAFHIQLAW
                     ADLLGDPELYALLGRYESALETRLLMARETTRRGRPSPNRSRREAFLWRSIEENHLGH
                     LESELAWARGVREGLGNLRYDPAREGSK"
     gene            20642..21502
                     /locus_tag="P5313_04360"
     CDS             20642..21502
                     /locus_tag="P5313_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY79630.1"
                     /translation="MRTEQKSVFKSEEGRRAVLAKYDEVLERWPEPGSVRDVPTRHGR
                     TRVVLSGPEGAPPAVLLHGSASNAVSWLGEAEILSRDFRVIAPDLPGEPGYSEEIRFP
                     LAGPAPEEWLDDVLAGLGLERILLAGMSLGGWAALRYASRRPGRVERLVLLCPSGAAP
                     ARASFLLKSVLYLAQGRKGVENLARLVYGNAEVPREAVDFTVLMMESFRPRMEAPPVL
                     ADAELARIPCPVLYVGGLKDALLRTRESAERLARLLPDFRAELDPEAGHVLPGRGGLI
                     SKFFGGDPSP"
     gene            21595..22368
                     /locus_tag="P5313_04365"
     CDS             21595..22368
                     /locus_tag="P5313_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY79631.1"
                     /translation="MLDFLVKGGPVLWVIIALSVAGTAIILERLLYFRRINTDEEKLF
                     LRVKSSVEKGHFDEALAICDNNLSPLSGLIRVGIEHRGYAETVQKDVLKDAANQEIPQ
                     LEKNVSALGTIAHIAPLLGLLGTVTGTMKAFGVLGQFGAVSDPSVLAKGVSEALITTV
                     AGIVVAVPAVIFYNYLVTKVNLILIKMENQVNTLILMINSVGRPAASARSESAARPES
                     AGRPESAGRPESAGRPMDASRGVSALSDWKTRKTREGGS"
     gene            22365..22781
                     /locus_tag="P5313_04370"
     CDS             22365..22781
                     /locus_tag="P5313_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRY79632.1"
                     /translation="MIIERRLKPRHTVDLTPLIDVVFQLVIFFMISSTFKVTPGIRLE
                     LPQSATAESVPLTEIHILAVSEGEIYLNKQQTSLEGLPVLLAKAVEGRAPDQMRAVLE
                     GDQGTSYQLMVSVLDALRKNGIEGVGLATKPNKGPK"
     gene            22778..23704
                     /locus_tag="P5313_04375"
     CDS             22778..23704
                     /locus_tag="P5313_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79633.1"
                     /translation="MTALNADPRQAYLRAEAKRRSLVSFGASAVLYLLALMILWIAGL
                     LRPVEYSEYAGPVLIRLGTPEGTEVPAPALPPVPDPVPEAVRPPEPTPAPAASVPAPS
                     PSQTARVPAAQTPASPPPSPTPIVPSTEGAVAGPIRGEEHGNSHETTFDATEGRVGRS
                     LYVPIWTFMPLPRSVEDRVYNRIQGDGFRTAVEQKTRFRNYYEFESSTWKLKQDVPPD
                     QRQGVWLILEGAGYDLSRADYKTGRVLSPIQIRFKVGPVQGNRSPVLESAELVVSSGD
                     PEIDTAVLFGFRRASFSNDSSRSVMGTFLYRF"
     gene            23704..24624
                     /gene="yqeC"
                     /locus_tag="P5313_04380"
     CDS             23704..24624
                     /gene="yqeC"
                     /locus_tag="P5313_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HRY79634.1"
                     /translation="MPSLQESLFSFPSAEQVPILALSGGGGKTTALFGLADELSARGA
                     RVLVTTTTRIRDPRAEGRGFDRFLEIPGAYATSEATAAVPLAAGLAADPAAAAPPGRG
                     PVTVLCAGVEGGKLVGLDPGIFPALAASGRWDAILVEADGARMRPVKAPAPYEPVWPA
                     GTTSALGVVGLDCLGRPLDDRVAHRPELLGALVGLEPGGTIELGHLLRLARHPEGLFR
                     GVPGGARRILVLNKADLRPDLDPGALADRTAAGLAEPGPAAPTGSALRPGPGTPGIDL
                     VLVCSLGDPDPRRRVRAAHKVAGVFKRREP"
     gene            24621..26252
                     /gene="yqeB"
                     /locus_tag="P5313_04385"
     CDS             24621..26252
                     /gene="yqeB"
                     /locus_tag="P5313_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005296144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HRY79635.1"
                     /translation="MNLYRIAADLEDRNRPFVLATIVESSGSTPRSRAKMIIEADGTC
                     HGTIGGGRVESRVIEEARACMASGRPRTLEFDLTGSGPDPDPMICGGRMTVLLEPGDR
                     KPRLLLVGAGHVNLAVARAAAPLGFRIAVVDDRPDLVTAERFPMASELFSGPTLREAL
                     GAALPADSALAASVPAEGNSVLIATASHASDEEALRHCIDKPCAYLGMLGSRRKTRVL
                     LSRLAESGVPEEALARVRAPVGLDIGAETPEEIAVSILGEILAERSGRGGGPLAGREG
                     DLVVVRGAGDLATGVILRLVRSGFRVVALESPRPTAIRRTVAFSEAVYDGSAEVEGLR
                     ARRAEGLAEVRAALAEGVVPVVVDPEAALVPALRPAVLVDAILAKRNLGTRVGQAPAV
                     IALGPGFEAGRDADAVVETARGHDLGRVILSGSAAPNTGVPGEVGGKSAERVVRAPVA
                     GEVIAVRSIGDPVRAGEPLLLIETPSGRVSVPSPLDGTLRGLIRPGIEAAEGEKIADV
                     DPRGRPEYCRTVSDKARAVAGGVLEALLYLRGRTK"
     gene            26302..>27180
                     /locus_tag="P5313_04390"
     CDS             26302..>27180
                     /locus_tag="P5313_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HRY79636.1"
                     /translation="MDTFDVVIVGSGPAGLGAAFEISSRDPGRSILMIDKEPFSTGGL
                     RNDCKMNFTWPIGFPENVWTEADGLSYLARVESFLKPDLMDRRNMDIYARRASKLGVR
                     LLEVRQAHLGTDGGLALIKDLTAELARRGVSISLGETAGRVEAEGRILVTDRREVGYR
                     DLILAPGRGGFSFLRHMMNDLGIPFQDNAVDIGVRVELKEERYAIVRDYYDPKFLFPK
                     KTRTFCTNSRCAHVVQEKYVTDDGRIWYSANGHAYSASRPANGLANFALLKTVTLTQP
                     LASGQDYASMLGVQASL"
BASE COUNT         4273 a         9269 c         9120 g         4518 t
ORIGIN      
        1 ccagcaggcc atcggcgcct ccctggggga ggactccatc gaggaaggca agcgtgcggt
       61 cctcatcggc cttctggcgg tcatggcctt catgctggtc tactataagg gcgcggggat
      121 caacgcggcc ttggcccagg tggtcaacct ctacctcatg ctgtccatcc tgtcgggctt
      181 caacctgacc ctgaccctcc cggccatcgc gggcttcgtg ctgaccgtgg gcatgtccgt
      241 ggacgccaac gtcatcatct tcgaacgcat caaggaagag ctcctggcgg gcaagggccg
      301 cagggccgcg gtcgaggccg gcttcggcaa ggccttctgg gcggtcatgg acggcaacat
      361 caccacgatc atagccgccc tcttcctcgc ccagctgggc tccggcccca tccagggctt
      421 cgcggtcagc ctggccatcg gcaacctctc ctccttgttc accgccctct tcgtgtcccg
      481 gctcctgttc gacttcggta cagaccgggg gctcaagggc gtttccatca gctggaggat
      541 caaatgaagc gcgtgattcg attcgaccgt ggattcctcg cagccgtgct cctctccgtc
      601 gccctgatcg ccttcggcat cgtcggcctg gccaccaagg gcatcaacct gggcgtggac
      661 ttccaggcgg gcatcaacca gtacgtccag ctggtatatc cggccctgga cgtctcctat
      721 tccgggacgg gcaacgcggt cctctcggtg agcgataccc gggcggtcct ggtcttctcc
      781 ggggcggacg tggagaaccg gaccatcgag tacgatctcg cagcctccgg gaccctgtcg
      841 gacctcgcct cggccttctc ctcccagccc ggcgtttccg cccgggtgac cgacggggcg
      901 ggaaagcccg ccgttaccct ggtaccgacc taccaggggg acatacgcct ggccgctgcc
      961 gccgtgcgcc tctcccggga acccgagacc gagaccgagt acttcgcggg catggacaag
     1021 gtgcgggccg cggccgccac catcggttcg gtttccgtcc agagcgtcgg ggagcgaatc
     1081 cggcagcagt acatcatccg gcttcgggac gacggcaccg atcccaagtt cagcaccacc
     1141 agcgcggaac ggatccggac cgccctggaa accgccttcg gggcgggccg ggtggtggtc
     1201 atgaagaccg atttcgtggg agcccggttc tccaaggacc tggcggacaa cgcgtggaag
     1261 ctgaccctgt tcaccgtgct ggccatcctg gcctacgcga ccgtgcggtt caagatccag
     1321 tacgccatgg gagcggtcct ggccatcctc cacgacgccc tcatcatgat cgccttcatc
     1381 gtctggaccc ggatcgagtt caacacttcc accctggcgg ccatcctgac gatcctgggc
     1441 tactccatca acgacaccat cgtcatcttc gaccgcatac gggaggaccg gcgcttgctg
     1501 cccacggagc ctttccacac ggtgctgaac aagtccatca ccgagaccct cgggcgcacc
     1561 gtgatcacca cgatcaccac gatgctcgcc gtgctgtcca tcttcttctt cacctccggg
     1621 agcatcaagg acttcgcgct gaccctcttc gtcggcatgg tgtccgggac gtattccacg
     1681 atcttcatcg ccaccgcctt cgtggccttc tggaacgacc aggcggagaa gcgcaagaag
     1741 gcgcagggca cggcgccgac ggccaagaag cccggaaccg ctccggttct caagccggcc
     1801 aagtagacat ctgtccgaaa gacgacggcc gccccgacgg gcggccgtcc tgtttttggc
     1861 tgccgcccga aggctttaca aaaggccctt tttttcgtat attcagtcgg cgcatggcgc
     1921 atggcgcatg gcgccgggcg gcagtagccg cgcggcgaga ggaagtgatt ataaaatgtg
     1981 ctatcgcgca tggcgccgtc cgtccgagta cggccgggcg gcgcaaggaa gcgattgtag
     2041 tatgtgctaa cgcacatggc gccgtaccca agcggtaagg gagaggtctg caaaaccttc
     2101 atgcggcggt tcgattccgc ccggcgccta aacgaaaagc ccccgtcagg gggcttttcg
     2161 tttaggcgca ctacaacaaa ttcctttcgg aaaacggccc gaagggccgt atggagcccg
     2221 gcgcctaaaa caacagccgc cccgaagggg cggctgttgt tttatcgagg cgagggcgga
     2281 atcgaagggt ccgagcgcgg cgcgatagcg ccgaagaggc gccaaggaag gcgccggaag
     2341 cgaggacccc ggcccggaga accgagccag gagggcgagg ttcgcagggc gattccgccc
     2401 ggcgcctaaa tgaaaagccc ccgtgagggg gcttttcatt taggcgcact acaatcgtta
     2461 cctttcggaa aggggcacga agcgccatgg agcccggcgc ctgaaacgac ggccgccctt
     2521 cggagcggcc gtcgttttag gcccagatcc ctccgttctc cgagcccgca tcgccgctcc
     2581 cgcccggacg gggcccttcc cggcgttcca tcggttcgat cgcctcgaag gccggacagt
     2641 gcaggcccgt cgcggcgaag acctccgcgg aggggaggtt ccgggacttg atcccgaaga
     2701 cctcgcagcc ccggggatac gcgggttccc aggtcacgat gaagtgtcgg caggccaggc
     2761 agttcggcgc gcgatccggg ggcagtcctt ggggcatcgg gctctccgag gttccggccg
     2821 ggttgaaccg accgtggatt ctccgtacat tatagaccat gaacagccga tcggtccttc
     2881 gccgtccctt ccgataccag ttccacaacg cgaccctggt cctgatcgcg gtcaacgtgg
     2941 gagtcttttt cctgacccag atctcccccg atttcctggt caacctggcc atggtgcccg
     3001 cctatgtggt ccgggggcgg ttctggctgc tcttcaccta catgttcgcc cacgggggga
     3061 tctcccacct cttcttcaac atgctcgggc tcttcttctt cggcacccat gtggaacgcg
     3121 agttgggttc ctgcgagttc ctgctgttct acctcctgac cggtgtcctg gcgggagcct
     3181 tctccttcgc ggcctacgcg gccacgggag cctggatcac cttcctgatc ggtgcctccg
     3241 gggccatctt cgcggtgctg ctggccttcg cggccctctt cccgggatcg atcatccacg
     3301 tctgggggat cctgcccgtt cgggccccgg tcctggtcct ggcgtacacg gccatcgaga
     3361 tcgcgtccca gctcttctcc ttccggagcg gggtggccca cctgacccac ctggccgggt
     3421 tcgggttcgc ctacctctac ctcctcgtcc gcttggacat caacgcgttc cgtaggttct
     3481 ttcccaggga ccgctgattc tcggaagcct ccatctggac ccttcgaagc gaggccgata
     3541 tactggaatc atgaacctgc ccgaactgga cgcgtggtgc cgctccttcc tggagatcga
     3601 ggcgttcaag aaggtcgacg actccctgaa cgggatccag gtgggacgga gcgaaggtcc
     3661 cgtcacccgg gtcgccttcg cggtggacgc ctgcgcggag acgatccgcc gggccgcgga
     3721 ggagaaggcg caggtcctct tcgtccacca tggccttttc tggggatccc cggcgcgcat
     3781 ccagggagcc ttgatggaga gggtgcggac cctgctggac cgggacatgg ccttgtacgc
     3841 ctgtcacctt cccctggacg cccacccgga gtacggcaac aacgcggtcc tggcccggat
     3901 gctcggactg tccggggtga agcctttcgg gttctacaaa gggatctccc tgggcttccg
     3961 gggtgtcctg gatccggctc cgacccttga ggaggcgatc cggaggatcc tcccggacgg
     4021 gagcaagccc cgggccgtcc tgcccttcgg tcccgaccgg atatccagcg ccgcggtggt
     4081 ctccgggggg gcgccccacg agctcttcga ggccgtggag gaggggctgg acctgtacgt
     4141 caccggggag ccttcccatt ccgtgtacca cgcagcccag gagtcgggga tcaacttcgt
     4201 ggccgccgga cactacgcca ccgaggtctg gggtgtccgg gcggtggccg aaaagctggc
     4261 ccgggagacg ggaacggaga cggtgttcgt ggaagtcccg acggggctct gatcatatga
     4321 accgggagag tatcgatgga tagcgacaag ccgaacagtc tccgcccctt cctcctgacc
     4381 gccgggatcc tgatcgcgga ccaggtttcc aaggccctga tcgtggacct gatcccccgc
     4441 aacaccatcg cggtccgtgg cctgggcgat tttctgtgga tcgtccatgc ccgcaacacg
     4501 ggcatagcct tcagcatggg ggacgggctt cctccctgga tgcgcatggt cctcttttcc
     4561 ctcctgcctc tggccttgat cgccggaatc ctcatctact acttccgcac cgacgagacc
     4621 accccggtgc agcgctgggc cctggccgcc atcgtggggg gagggctggg aaacgtcgtg
     4681 gatcgtcttt tccggccgga gggagtggtg gatttcgtca gcgtgaaatt ctacgggctt
     4741 ctcggcatgg aacggtggcc gaccttcaac gtcgcggact ccgccgtcgt cctgggggcc
     4801 ctcctcgtcg cggccacgag cctgcttgcg cccggtcccg ggactccggt ccgggacaag
     4861 gacggcggat cggccggatg agggatccga agggcgatcc ggttcccgcc gcccggaccc
     4921 ccggcgcccg ggcgcgggga atcctgctct tcctcgcggc cacgatcctg aacttcctga
     4981 ccgcctcggc cttcttcttt ggattcctgg ccctctgggg actcgtcctg gcgccttggc
     5041 tcaagctccc cacgtcctct ccggtgatcc tcgcggcctt cgtcctggcg gtcgcgggat
     5101 ccggcatcct ctaccgcgcg gcggtcaaga tctacctccg gaagaccggc cgggccccgg
     5161 gccatttgac gaaatcctga ttcggctgca tactcgaatc cccccatcac gatcacgcaa
     5221 ggagtcttca tatggcgtcg ttcgaggata agctcgcctt ggcgggcgtg cgagtgccgg
     5281 aggtccgcct gcccgcccgc ggcgcggact gggaggcctg ggccgtggtg gcctgcgatc
     5341 agtactccag cgagcgggag tactgggaag gcgtcaagga ccgcatcggc ggcaagcctt
     5401 cgaccctgaa cctcattttc ccggaatgct acctggagga ctcggacaag gacgagcgga
     5461 tcctgcgcat ccaggcggcc atgcgggact acctggaccg cggcgtgctc gaaagcgccg
     5521 gagagggctt catcctggtc gagcgccgga cccccttcga ggcggagccc cgcaagggcc
     5581 tcatgatcgc cgtggacctg gagaagtacg agtacggcaa gggctcggcc tccctcatcc
     5641 gcccgaccga gggaaccatc gtggagcgcc tgcccccgcg catgaagatc cgccgggggg
     5701 ctcccctgga gctcccgcac atcatgctcc tgatcgacga ccccggccgg acggtcatcg
     5761 agcccgtgta cgaggcccgg gagaccctga agccgatcta cgatttcgag ctgatgaccg
     5821 gctccggcca tgtccggggc tggcaggtcc ggggcgaggc ggccctgggc gggatcgcgg
     5881 acgccctgac ccgcctggcc gatccggcgg cgtacaagcg gaaatacggc aaggacgggg
     5941 tcctcctgtt cgcggtgggg gacggcaacc actccctggc cacggccaag gcggtctggg
     6001 aggagatcaa gggggcgaac gccgggaagc ccgggatcga gcgccatccc gcccgctggg
     6061 ccctggtgga gctggtcaac atctacgacg agggcctgcc cttccacccg atccaccggg
     6121 tcctcttcca ggccgacgag aagaccttcc tggcggagct ggccgcgcac ggggactgcc
     6181 gggtcgagga ggcctccaag gccgcggcgt tcgcggccgt ggacgagggg gccgcggagc
     6241 accgcttcgc catcctgacc gcgggcggga cccggctggt ccgcttcccc cggcccaagg
     6301 ccaacctggc cgccgggacc atccaggagt tcctggacgg gtacctggcg aggcaccccg
     6361 ggaccggcat cgactacatc cacggccggg aaagcctgga aaccctggcg gccaagcccg
     6421 ggaacctcgg catctacctg ccccccatcg acaaggccgg gttcttcggc accgtcatcc
     6481 gggacggggt gatgcccaga aaaaccttct ccatgggcga ggcgccggaa aagcggttct
     6541 acatcgaggc gcggaagata ctgccgtagg ggtcgtcctt gacaccccgc ggcggcatgg
     6601 taagatggat cgataaaggg aagaaccaac cgatctccag ggatggcccg cgtaccggag
     6661 cccgaggctc cggggcgctt cggccgcccc ggacggccgt ctttccttga tcagtatcaa
     6721 gcatcgagtt ccaggaggtt ttcatggtta tcctgcttgc ggacgccttc cttcccgacc
     6781 tgccgaagcg cctggcggcc ttcggcgagg tgacccagga catgggccgc gtgggggagg
     6841 cggaggtgat gatcgtccgg tccaagacca aggtcgggaa ggacgtgata gacaaggccc
     6901 cgaagctcaa gttcgtcatc cgcggcggcg tgggggtgga caccatcgac gtcccctacg
     6961 cggagtctaa gaagatcaag gtgacgaaca ccccggaagc ctccagcgtg gccgtggcgg
     7021 agctggcctt ctccatgatg ctggccctgg ccagccggat ctgcgaggcc gacgcctcca
     7081 tgaagcaggg caagtggctc aagaaggacc tggagcggac ggagctcatg ggcaaaaccc
     7141 tgggcctggt cgggatcggc cggatcgccc gggaagtcgc cctccgggcc aaggccttcg
     7201 gcatggaggt catagccttc gacaagttcg tgccgaagag cgacgtcgcc gcgatggtct
     7261 ccctggagga ggtctgggcc aagtcggact acatctccct ccacgtgccc ctgacggacg
     7321 agaccaaggg catgatcaac gcggcggcca tcgccaagat gaagaagggc gtccgcgtcg
     7381 tgaacacctg ccgcggacag gtcgtctccg aggaggacgt ggccgcggcc ctggccgacg
     7441 gccgcctggg cggctacggg gcggacgtgt tctactccga gccccccgag ggctcgcccc
     7501 tgctctcggc cccccggacc gtcctggcgc cccatctggg cgcctccacg gaggagaacc
     7561 tggcccgact gggggactgc atcgtggaca ggatgaagga atacgtgaag aagtgaatag
     7621 gtgaatacgt gaataggaga gggtcgggtt tccgttcgta cgccctattt acccattcac
     7681 ctattcaccc accctactag aaggagccga atatgaaacg cgtcatcaac tacagcgccg
     7741 gacccgccgc aattcccgtc gaggtcctcg agcaggcccg ggacgagatg ctggactgga
     7801 acggcacggg ctgctccgtc atggaggtgt cccaccgcgg caaggagtac gacgccctcc
     7861 acatggagac catggacctg ttccgggaga tcgccggcct cggcaagaac tacgaggtcc
     7921 tgttctgcac cggcggggcc agcacccagt tcctcatgct gcccatgaac tacctgggcc
     7981 cgggcaagga agcggactac gtggttaccg gcaactggtc caaggcctcg atcaaggagg
     8041 ccaagcgctt cggcaagatc accttccccg ccaacatgga gctggagggt gagcgcttcg
     8101 tggccatccc taagcagggg gacctcaagc tgaaccccgc ggccgagtac gtccactaca
     8161 ccagcaacaa cacggtggaa ggcaccgagt ggcactacat ccccgacgtg aagggcaagc
     8221 ccctggtgtg cgacatgtcc tcggacatct tcagccgccc cctggacttc aacaagttca
     8281 gcctgatcta cgcgggcgcc cagaagaacc tcgggccctc gggggtcacg gtcgtggcgg
     8341 tgcgagacga cttcctggcc aaggcccacg acgacctgcc cacgatgatg tcctacaagg
     8401 tgttcaagga caagttctcc atgcacaaca ccccgccctg cttctccatc tacatcctga
     8461 acctggtgct caagtggctc aagaagaacg gcggattggc ggccatgggc aagacgaacg
     8521 agcggaagca gaagctcctc tacgccgccg tcgacgggtc caagggcttc tacaagggcc
     8581 tggcggaggt ggattcccgg tcctggatga acatcgactt caagctcccc acccccgagc
     8641 tggacgacaa gttcgtcaag gaggccaagg ctgcggggat cgtccagacc aagggctacc
     8701 ggagcatggg cggcatccgc ctgtccgcgt acaacgcggt gtccttgaag gacgtcgaga
     8761 ccaccgtggc gttcatgcag gacttcatgg cgaagaacgg gtaatacgtt agcaggatct
     8821 caccacggag tcgcgcgagg cccgcacggc gggcctcgcg gcgccgaagc gagcttcggc
     8881 gatcttctgt tcaaccaaac tcggcgaagc ccgaagggct tcgctgccgc gagggcggct
     8941 ttgccgccct cgcgcgtctc tgtggttgat tcctgaaaaa tcctatttcc ggatcctcgc
     9001 gcgaagggcg tcgatgcggc cgcgcaagcg ctcggcgtcc tccttggtag cccgtacgaa
     9061 ggggttgccc tcgggggatc cgcacacggc ccgcagttcc tcgggattcc ggaaggtggc
     9121 ccgtcggaag gggttggaat agtccttggc ccggctcagg tagacctgct cctccacata
     9181 ccgggaaccc tcctccagga ggtccagggc cttgcccagc tcccggggtc cgatcgaggt
     9241 ccagccctcc aggtcccgga gacaggccca ggcgtggcgg gccttgtcca cggggtactc
     9301 cgaggccagg cggccgtagg cggcgtggat ctcatcccag gatcccaaga ccccggatcg
     9361 tatctccgcg atcagggctt cgaccttgcg ggccgggacg atctggccgc ccaggttctc
     9421 ccattcgcgc tcacgggggc cgtccagggc ctccgccgcg tccgagaccg tcatgtccgg
     9481 gcgggcttcg aagtacgcga ggatctcccg cacggcatac cagtggagca tccggcggta
     9541 ggccttccag gcacgccccg ccttgaggat caggaccggg cgcgtggagt tttccacccc
     9601 gtaggcgtgg accgcgacct tgccgtcctc gggtccggcc aggagggccc ggccccgctc
     9661 ccggagggcc tccggccccg gggcggaggg atcgccgccc tccgcgagga ccgccgccgt
     9721 gcccgcccac tcctccagga ggacccgggc ccggaggatc tcctccgcgg tatccggggc
     9781 gaggggggag agctccaggg cctggacctt cgtgatccgc ctgtcccggg cggcgaactt
     9841 ggattcgttc cgcaggaggg cgtagaggtt gtacatccac cagtacgccg ggatcagctc
     9901 gatccggtcc ttggcgggat tctcgttgac caggcagaag ggaaggggga tgtccagttc
     9961 tgcggggtag tcgcccttgg acagcaagac gtaggaggcg aagcggcagg agtgcttgag
    10021 ggaagtggac aggccgggcc agaaaccgcg ggccgcctgg atctccccgt cgttggcccg
    10081 ggaattgtgg ttcgagccga tggtgcagcc cgcggccagg ttggactggc cctggaccac
    10141 gctggcgatc aggaagctgg tgttgtggtg ctgttcgtgg gccgggaaga tcaggttgtt
    10201 gaggagttcg cagcaggaga cggtggagtt gtcccccagg acggagtgga tcaggcgggc
    10261 cccgtacttg agggccgagt tgttgcccat gatgaatcgt atggccttga ccccgtagaa
    10321 ggcccggcag ccgtacccga cgatgccgtt gaccagctcc acgccctccc cgatctgggt
    10381 ccgctccgcc tcgctggagt tgatggtgat gttcttgagc ttgttggcgc ccttgatata
    10441 ggcgcagtcc ccgatccggg cgtccttgat gatccggttg tgcttgagca cgcttcccgc
    10501 gccgatccgg ccgtagccgc ccctccggtt ctcgaagccg gcctgtgtca gggccttgaa
    10561 ccgttccatc agggccgaat cgtcccgacg cttggcccat agccaggcgt cggcgcagat
    10621 catcccgtcg aagggcagga cggaccggcc cccggcctcg ttcatcaggt cgatccagac
    10681 ccggacggac tcctcctccc cctccatcac gaccccgttg ccgaacttgg cgtggttcgt
    10741 ggcgtgtatt tcattgttgt tgaggagaat gcaggaatcc ccgatctggt agtgggctat
    10801 gtagtcgcag tcgtggatag cgcagaggtc cccgatgtcg caggcgacta tccggctgtc
    10861 cgtgatgccc gcgggggtct ccaagtcgtg gtgggacagc accacccgtt ccagccgccc
    10921 gatgcggacc aggccggcga agtcgcagcc ccggatcagg tccgggatga aggggtcccg
    10981 caccaagata cccgaccagt ccgtgcaggt attcccgttc cgggccaggg tttcgatctc
    11041 cgcggcccgg agggggcggt acgagtccaa aggctttccc agcgtggaat tacgaagatg
    11101 gtactcgtcc ttgccgggag gcaggaaccc ggaggggatg aaggagcggc cgtatccggt
    11161 ctcgtccgag agggtgatat cgttcatgcc cgaaagtgta tcccgaaacg ggcgggctgg
    11221 ccagcgaggc gggccggttg acaaagaaag cccctccggg acatggtaag ccatgtcggt
    11281 tcccgaaatc ctggccgaag atcccatcgc ggtggacgtg gaaaaagccg cgtacgagat
    11341 attccccctc ctggtgcagg ccgaaaacag ggtcctccgc gaacaatacg ccacagcctt
    11401 cgccaatctc ctgggcaacc ccggggagtt ctataccatc gtctccgggt ccatgggcga
    11461 gcgcctggtc cacgtggaac ggcttctgca cgccttccgt gaaaacgtgg aactcctgat
    11521 ccgcaagacc tgggtggaga agcacgacga gaagagcaag gaaaaggtcc acgaagacct
    11581 ctccgtcttc gtacaggagt tccaatccgg ggcggtgcgc aaggccttcc cccatttcgt
    11641 gtccctgagc ctgggtatag cccggctcct cttcggggcc caggcccagg ccccggactt
    11701 cctggaatac accttccgga tcgatcccaa gctgggggtg ttctactggt ttatcggccg
    11761 cctcaaggag caggaatccg ccgaggtcgc cgaggacctc cggaccctgg agctcctcct
    11821 gggggtcttc gccctggcct ccttctaaag cccatgcccg acgcggggtc cctccgatcc
    11881 gtccgcctgg acgaggaagc gggggaactc gagctcatcg accagaccct cctgccgaac
    11941 cggaccgtcc tcctccggct ctcggatccc cgggacatcc gggaggccat ctacagtctc
    12001 cgggtccggg gcgccccggc catcgggata gcggccgcct acggcgtcta cctggcgatc
    12061 cggagaagcg cagcccggga ttgggatgcc ctggccgtgg aattccgggc cgccaagggg
    12121 ctcctggcct cggcccggcc gaccgcggtc aacctctcct gggccctgga ccgcatggag
    12181 cggaggctgg atgccgcccg gggccgcccc gtcggagagg tcaagaacgc cctcctcgag
    12241 gaggccgagg ccgtccgcgc cgaggacgag gaggcctgcc gcgccatcgg ggagaacggg
    12301 ctttcgctcc tgggccccgg ctggggcatc ctgacccact gcaacgcggg atggctggcc
    12361 acctcccggt acgggaccgc cctggcgccc ctctacctgg gggccgaacg cggctacggc
    12421 ttccgggtct tcgcggacga gacccggccc ctgctccagg gcgcccggct gaccgcctgg
    12481 gagctggatc gggccgggat cgacctgacc ctgatctgcg acaacatggc ttccaccgtg
    12541 atgaagcgcg gcctggtgaa ggccgtcctg acgggctgcg accgggtggc ggccaacggg
    12601 gatgtcgcca acaagatcgg gacctccggg gtcgcggtcc ttgcccggca ctacgggatc
    12661 cctttctacg tcctggggcc cgtctcgacc gtcgacctgg cctgcccgac cggggcggaa
    12721 atccccgtgg aggaacgccc ggctgaggaa gtgacggacc ggtggtacgt ccggaggatg
    12781 gccccggagg gtgtgaaggt cctcaatccc gccttcgacg tcaccgacgc cgggcttata
    12841 accgccatcg tcacggagcg cggcatcgcc cgacccccct acggggattc cctggcgcgg
    12901 atcgtccagc cgtaagctca ggccccctga ttgcgcttgg gctggaagtg gctgaactcc
    12961 atgctgaagc ccgcccgccc ctgggtcacg gagcgcaggg ccgtggtgaa gccgaacatg
    13021 acggacaggg gagcctcggc gtggatggtg tcgctggcgg ccttggagtc cgtgccgtgg
    13081 atcatcccgc cccgctggct caccaggctc atcgcctcgc ccaggaagtc cgagggggtg
    13141 acgatgtcca ccttcatgat gggctccagg aggaccgggg cggccttccg gcaggcctcg
    13201 tcgaagccca gggccgcggc cgcctggaag gcgtaggggc tggcggtcag ctcgtcccat
    13261 tccaggtccg tcagggtcac ccccacgtcc acgcaggggt agccgaactg gatcccaccc
    13321 gcgaaggcgt tcgtgaccgc cgtctccacg gcctcccaga tctcgtccgg gaccttgggg
    13381 ttccggatgt ccttccggta gaggttgccc tccccccggg cccggggttc cacccgcagg
    13441 gtgagccggg ccgcgttctc cttgcccgcc aggaccttgc ggaaggcctc ggtgtgctcc
    13501 accgccttgg tcacggactc ccggtaggtg acttgaggat tgcccacccg ggcgtccacc
    13561 ttgtagtccc ggatgatccg ggtcaccagg acgtccaggt ggagttcccc catgccctgg
    13621 atgatgagct ggccggtctc gtcgttttcc ttgctggtga aggtgggatc ctccaaggac
    13681 aggaggtcca gggtggactt gagcttgtcc cggtccgaga gggtcttggg ctcgatggcc
    13741 acggagatga cgggctccgg gaagtgcatc cgctccagga ccacgggcca gccctcggag
    13801 ccgatggtgt ccccggtctg ggcggctttc atgcccacga tcacggctat gtccccggcg
    13861 cccacttcgt ccaggggctc gctcttgttg gcgtgcatcc tgagcaggcg ggtgacccgc
    13921 tcgcgcttct tcttgccgat gttgtagacc gtggaggcgt tcttgacgga acccgagtac
    13981 atccgcacga agcatagggg accggcctcc cggtcgtact ggaccttgaa cacgagtccc
    14041 aggggattcc ccgcggactc gcaggggacc tcgatgtcct cctccttctt gaggtggaag
    14101 gcccgggccg cggggacctc gtggggggag gggaggtagt ccaccaccgc gtccaacagg
    14161 gcctggacgc cgatgttgcg acggctggcc ccgcagagga ccgggacgat gttccgggcc
    14221 agggtctgtt tccggatctc ccggcggagg agttcgggcg ggacctcgcc gccggccagg
    14281 aagagctccg cgatctcgtc ggaccaggtc cccagggagt ccatcagctt ttcccgccag
    14341 gcgtccgcct ccgtggcccg ctcgggggac agaggcgtcc gggtgatgtg ctggcctcct
    14401 tcggctgcgt cccagcggag ttcctcccgg gcgaggaggt ccaccatgcc ctcgaaggca
    14461 ccttcccggc cgatcgggat ggccaccggg acgggattgg ctcccagctt ggtccgcatg
    14521 tcctccagga cggcgaagaa atcggctccc agacggtcca tcttgttcac gtaggcgatg
    14581 cgggggacgt ggtagcggtc cgcctggtgc cagaccgtct cgctctgggg ttccacgccg
    14641 cccacggcgc agaatacggc tacggccccg tccaggaccc gcagggaccg ctccacctcg
    14701 gcggtgaagt ccacgtggcc cggggtgtcg atgatgttga tctggtggtt ccgccagaag
    14761 gtggtggtgg cggcgctggt gatcgtgatc ccccgctcct gctcctgctc catccagtcc
    14821 atggtggctt ccccgtcgtc cacctccccc aggcggtagg tcttgccgga gtagtagagg
    14881 atgcgctcgg tagtggtggt cttgcccgcg tcgatgtggg ccatgatgcc gatgttgcgc
    14941 gtcgtgtcga ggtccatggg atgctccagg ataaggattc cgctcgacag cctcgaaagg
    15001 gaaagccggc cgcgggataa cttacggtac tatatacgaa ataccgcagg gttttcaagg
    15061 acgggaaagt gtgataaggt gtccccatgc cttcttccct cgaactcctc gcccccgcgg
    15121 gtaaccccga agccctggac gccgccgtcg gcgagggcgc cgacgccgtc tacctgggcc
    15181 tgcgctcctt caacgcccgc atgaggtcgg ccaacttcgc ctacaaccag ttcgaggccg
    15241 ccgtggaggt cctgcacaag cagtccaagc gggtctacgt cacggtgaac accgtcttcg
    15301 aggaacggga agcggaccgg atgtaccagt tcctccagta cctggacaag gtggggcccg
    15361 acggcatcat cgtccaggac ttcggggtgg tgaagatggc ccgggatcat tttccctccc
    15421 tgcgccttca cgcctccacc cagatgaacg tggccggcgc cgcgggggcc aaccagcttt
    15481 cccgctgggg cttcaaacgg gttgtcctgg cccgggagct gtccctggac gagatagccg
    15541 ccgtgcggag gtccacgaac ctggagctgg agaccttcgt gcacggggcc ctctgcgtca
    15601 gcgtctccgg gctctgcctc ttctccagct ggctgggggg caagagcgcc aaccggggag
    15661 gctgcgccca ggcctgccgc cgcctctatt ccaacgaccg ggagacgggc tactacttct
    15721 cccccgacga cctggagctc atcgcccggg tgccggacct ggcggaggcg ggggtctcgg
    15781 ccctcaagat cgagggacgc atgaagagct ccgagtacgt cggcaccgtg gtgggcgcct
    15841 accggtacat gatcgacaac taccggctcg accccgagcg cgccttggcc aaggcccagg
    15901 ggatgctccg caacgacttc gcccggcgca agaccaccta ctggttcgag gggaccgccg
    15961 cccccgactt catccggccc gaccaggcgg ggggcacggg catccccctg ggcaaggtgc
    16021 gggaagtccg cgccttcgag ggcaagctcg agggccgctg ggccctgctc aacaccttcg
    16081 aggggatcgc ggagggcgac tccgtccggg tccatcgggc ggacgattcc ggccgggaga
    16141 ccggcaaggt caaggaagtg ctgcagaagg ccgaggggat gtatctccgg ttggagctgc
    16201 ccttccgcca gaacgaccag gtctacctga tccagacccg cagcatggcc cgcaggtaca
    16261 agagcgtcct gccctccagc ctggagcgtt tccggcgctt ccccagctac gactccgccc
    16321 cccgggtgga gttccccaag ctccccgcgg agcggctgga ctcgttcccc gcggggatct
    16381 acgccctggt cggccggacg gcggatctcc acgtcctcct ggcttcccgg cccgtgaagg
    16441 ccatgctccg gttgaaccgg cgcaccgccg cggatcttag ggcctcggag agcggagcgc
    16501 ctttcaagaa ggattccctg gtactctggc tggatccctt cttcccggag ggcgacgccg
    16561 cgtggctggc ggaggagttg gactattgga tctcccgggg tcagcggttc ttcgtggtca
    16621 acaacctcgg ccacctcaac ctgctcaaga accggggggt ggtcctggcc gcggggccct
    16681 ggctctacgc cttcaacgta cgggccgccg ccttcctctt cgagaacggg gtggactgcc
    16741 tgatcccgcc ccacgagatc tccaagcagg ccctccagca gatcgccgag gtcatccctc
    16801 cccgggcgat catgcccctg gccttctcct tcccgcccct gttcacgatc cggtccgacc
    16861 tggcttcgga gtacaagttc cggaatttct ccgacaagga ggggaccgcc tacgaactcg
    16921 ttcgggacgg caccgggagc gcggtcatcc cctcccggcc cctgtccctg acggaccggg
    16981 tccccttcct gcagaaggac gggtacgaga agttcctcct ggacttcgga cacatggagc
    17041 tggccaagcc catgtacaag caggtcttcc aggccgcccg ggaaggcatc gttctgcccg
    17101 agacgggccg cttcaactgg aagagcgggt tctggaaccc cgacgaggga tccccggccc
    17161 gggacgattc caagggaggc ccggggcgcc gaaccgcggg cgcgggaacc aggtccggcg
    17221 ggccgggccg gtccgcgacg gccgggaggg tcggacgatc ggccggacgg gacgcctcgg
    17281 accgcccgga tgcggcgggg aaacccggaa cccgccggcc ccgaagcggg ccggaaagcg
    17341 ggacctcgga cagaaaaccc gcgcggggcg gccgccgtcc cgccggacgg ggaccctcgg
    17401 ccggtccggg gacaggctcc gggtccggac cctcccggga ccggaaaccc cggaccggcc
    17461 cgaagcgccc cggcgcgggc aggaaatccc gggattagcc gcgccccggc cttacagggt
    17521 gcggacatgc ttcgagtttg tcagcaccct gcgaatccac acggttccga aaaaaccttc
    17581 ttgacgaata tccaaaccgg gtgtagtcta aacccgttgt ttcaataacc gatattcagt
    17641 ttcttataga gaaacaagcc ctgttcccga taggtcgtac atgagcggat ccacggtcaa
    17701 ggcattcgag ctgctgacat acctctcccg agccggagaa gcggtcactc tggcccggat
    17761 ctcccgggag acgggctggg acaaggccac cgccctccgg tacctgggat ccctggccga
    17821 ggccggggcc gtggagaagc gggagaacgg ctgggtcttg ggcttggccc tgatcgagct
    17881 ggccagccgg gttccggtgt cggagaacgt cgcggcccgg gcccgggccg tcctggagcg
    17941 cctggcccgg gagaccgggg agactataaa cctcggctcc tggagcaacg gcggggcgta
    18001 ttacctggcc aaggcggacg gcgggcgcaa cctccgtctg cgcacgagcc cgggtgaccg
    18061 cctgcccctc cactgtaccg cggtgggcaa ggcgatcctg gccaccctgg attcggccta
    18121 cctggaggat ttcctccagc aggcgccgta cccgaggctg acccccacca ccatcacgga
    18181 ggcacaggcc ttccgggagg agatcggcca ggttcggaac gtggggtacg ccatggaccg
    18241 ggaggagtac gagccgggcc tggtctgcat cgcggtgccc atggaaatcc ccgccctggg
    18301 gttcctggga gccgtgagcg tgtccggccc ctcggtgcgg atgcgggacc ccttcgggtc
    18361 catcctggac ctgctgcggg ccgcgtcccg ggacatcgtc gcggccgtca cggctgccca
    18421 gaccggggag gccggggagg attgagggag gccggggccg cgtggacgcg cggaggcccg
    18481 gcaggctgtc gaaatgctta cgtatttcaa caacctacta cgttgttcgg tgcgcgggaa
    18541 ggccgtcggc cggcccgcga ttccgatagg gggattctgg gggcttccgg acgtcgttcg
    18601 ggagcccccg ctttttctcc ggcgcggtca gaagacctcc gccggctcct ccttgcgtgc
    18661 ccgggacgag gggggacgct ccccggaggc gtcgtccttc atctgcggct tgaactccac
    18721 gtgctccgcc acgattttga ccttgttgtg cgacttcccc tccccgtcgt tccaccggtc
    18781 ctgcttgagc ctcccaacga cccgtacccc ccgccccttc ttgagggtgt ccccacaggt
    18841 ctgggccagc ttggaccagg cctccacgtc gaagaaggac acctccttct ccagctggtc
    18901 gttctgcttg taccaccggt tgctggccac cgagaaggtg cagaccgggg ttcccgaggg
    18961 ggtggccttg aggtccgggt ccctcaccag gttcccttcc agcaggatcg aattcaggtt
    19021 gttcatgccc tctccttccg cgcggcatcc gcgcgacctg tcccgccccg cggggcggtc
    19081 cgccggccgg ggaccgggcc ccgcgccccg tccctttcgc cggcggggta gtaccgccgg
    19141 gaaccccgag ttcgcttgcg tcccggaggg aaatcgagcc cggccgctcg ggggcggcgg
    19201 gcggactgaa cgccggggcg gcttggacgg cgcgggcccc cgggctatac tgatccgcat
    19261 ggatccatcc cgccccggcc gagcccggcc cctgtctgcc gcccttcgga tcgcagtctt
    19321 cgcggcgacc ctggcttcct gcgcgggccg cggcggaagc ccttccgcca aggacgccgc
    19381 ctaccccttc ccggagcggg agacctacgc ccccgccgtc cccacgaagc tccagacggc
    19441 ggtggtttcc ggcgggctct ccctggtcgg caggagcgaa ctcgtggtga acgggacccg
    19501 gtaccccatg gactgtacgg gggtggtccg ggcggcctac gccttcgggg ggatggacct
    19561 ggcgtaccgc ttcgcctttt atccgggcaa cggggtggcc cggctctacg ggaccctccg
    19621 ggacgcgggg ctcctgtact cgacccgcta tcccgccccc ggggacctcg tgttctggga
    19681 cgatacctac gacgcgaacg ggaacggccg ggccgacgac ccgctcaccc acgtgggcat
    19741 ggtcgtggag gtcgacgagg aggggaccgt ctcctacctc caccacaact accgccgggg
    19801 tcccgtggtg gagcggatga acctgatcca tcccggcgtg gaaaccctct tcgtgaaggg
    19861 ggtctccgtg acggtcaact cgccgttgcg catgcggggc tctcccccgg gaccggggac
    19921 caccgcgggg gagctcttcc gggtcttcgg ggcggcgtat aagctttccc gataagtccg
    19981 acaggatgaa caggatggag tagaatgaag gaattcttct tgacagatat gttaaaagta
    20041 acatagtata ataataacta agttgccagg gggctattat gtccatccag cacgcgatcc
    20101 tgggcttcct gagctggaaa ccccatacgg ggtacgagct caagaaggtc ttcgcggatt
    20161 ccctgtcctt ccactggtcc gggaacaaca accagatcta cgggaccctc atcaagctcc
    20221 accaggaagg cctggtcacc aacgaggtgg tctaccagga caagtacccc gcccgcaagg
    20281 tctatacggt gactccgaag ggcctggaaa ccctgcgggc ctggctgggc tcggagccgg
    20341 agctcccgga gctccgcaac gccttccaca tccagctggc ctgggcggac ctcctggggg
    20401 atccggagct ctacgccctc ctggggcgct acgaaagcgc cctggaaacc cggctgctca
    20461 tggcccggga gacgacgcgg cgcggccgtc cgtctccgaa ccggtctcgg agggaggcct
    20521 tcctctggag gagcatcgag gaaaaccatc ttgggcacct ggagtccgaa ctggcctggg
    20581 cccggggcgt gcgggagggg ctgggaaacc tgcggtacga ccccgcccgg gagggctcga
    20641 aatgaggacg gagcagaagt cggtcttcaa atccgaggaa ggccgccggg cggtgctcgc
    20701 gaagtacgac gaggtactgg agcgctggcc cgagcccggt tcggtccggg acgtcccgac
    20761 ccgccacggc cggacccggg tcgttctctc gggccccgag ggggccccgc cggccgtcct
    20821 gctccacggc tcggcctcca acgccgtgtc ctggctgggg gaggcggaga tcctgtcccg
    20881 ggacttccgg gtcattgcgc cggacctgcc cggggaaccc gggtacagcg aggaaatccg
    20941 gttccccttg gcgggtccgg ccccggagga gtggctggac gacgttttag cgggcctcgg
    21001 ccttgagcgg atcctcctgg cggggatgtc cctgggcggg tgggcggccc tgcgctacgc
    21061 ctcccgccgt cccgggcggg tggagcgcct ggtcctgctc tgtccctccg gggccgcccc
    21121 ggcccgggct tcgttcctgc tcaagtccgt cctctatctc gcccaaggcc ggaagggggt
    21181 ggagaacctg gcccgtctgg tctacgggaa cgccgaggtc ccccgggagg cggtagactt
    21241 caccgtcctc atgatggagt ccttccggcc ccggatggag gcgcctcctg tccttgccga
    21301 cgccgagctg gcccggatac cctgtccggt cctctacgtc gggggactga aagatgccct
    21361 cctgcgtacc cgggaatccg ccgaacgcct ggcccggctc ctgccggact tccgggcgga
    21421 actcgacccc gaggccggcc acgttcttcc cggccgaggc gggcttattt cgaagttttt
    21481 cgggggcgat ccctccccct gaatcccgag tcctgggtcc tgtgtcctga ctaccgccgt
    21541 ccttcgcctt gaaaccatcc gggtatgagg gtattttaag aaggaggagg aaccatgctg
    21601 gactttttgg tgaaaggcgg ccccgtcctg tgggtgatca tcgccctctc ggtggcgggc
    21661 acggcgataa tcctggagcg gctcctctac ttccgccgga tcaacaccga cgaggagaaa
    21721 ctcttcctgc gcgtgaagtc ctcggtggag aagggtcact tcgacgaagc cctggccatc
    21781 tgcgacaaca acctctcccc cctgtccggg ctcatccggg tggggatcga gcaccggggc
    21841 tacgccgaga cggtccagaa ggatgtgctc aaggacgcgg ccaaccagga gatcccccag
    21901 ctggagaaga acgtctccgc cctgggcacc atcgcccaca tcgcccccct gctgggcctg
    21961 ctgggcaccg tgaccggcac catgaaggcc ttcggcgtcc tggggcagtt cggagcggtc
    22021 agcgatccct cggtcctggc caagggcgta tccgaggccc tgatcaccac cgtggcgggg
    22081 atcgtggtgg ccgtgccggc ggtgatcttc tacaactatc tggtgaccaa ggtgaacctg
    22141 atcctcatca agatggagaa ccaggtgaac actctgatcc tgatgatcaa ttccgtgggc
    22201 cggcccgccg cctccgcccg ctccgaatcg gccgcgaggc ccgaatcggc cggcaggccc
    22261 gaatcggccg gcaggcccga atcggccggc aggccgatgg acgcgtcccg gggggtctcc
    22321 gccctttcgg actggaagac acgtaagacc cgggagggcg gctcatgatc atcgagcggc
    22381 gcctgaagcc ccggcacacg gtggacctca cgcccctgat cgacgtggtc ttccagctgg
    22441 tcatcttctt catgatctcc agcaccttca aggtgacccc ggggatccgg cttgagcttc
    22501 cccagtcggc cacggccgag tccgtacccc tgacggagat ccacatcctg gcggtgtcgg
    22561 agggggagat ctacctgaac aagcagcaga cctccctgga gggcctgccg gtcctcctgg
    22621 ccaaggccgt ggagggcagg gccccggacc agatgcgggc cgtcctggaa ggcgaccagg
    22681 ggaccagcta ccaactcatg gtgtccgtgc tggacgccct ccggaagaac gggatcgagg
    22741 gggtcggcct ggcaaccaag ccgaacaagg ggccgaaatg accgccctga acgcggaccc
    22801 ccgacaggcc tacctgcggg cggaggcgaa acgccgttcc ctggtttcct tcggtgcctc
    22861 cgcggtcctg tatctgctgg ccctgatgat cctttggatc gcgggactgc tccgccccgt
    22921 cgagtactcc gagtacgcgg gccccgtcct catccggctc gggactccgg agggaacgga
    22981 ggtccccgct ccggccctgc ccccggtccc cgaccccgtg cccgaggccg tccgtccccc
    23041 cgagccgacc ccggcccccg cggcctcggt tcccgcgcct tccccgtccc agacagcccg
    23101 ggtaccggcg gcccagacac cggcctcccc gcccccgtcc cccaccccca tcgtcccctc
    23161 caccgaggga gccgtcgcgg gacccatccg gggggaggag cacgggaatt cccacgagac
    23221 caccttcgac gcgacggaag ggagggtggg gcggagcctc tacgtgccga tctggacgtt
    23281 catgcccctg ccccggtccg ttgaggaccg ggtctacaat cgtatccagg gggacggatt
    23341 ccgcacggcc gtcgagcaga agacccggtt ccgcaactac tacgagttcg agtcttcgac
    23401 ctggaagctc aagcaggacg tgcccccgga ccagcgccag ggtgtctggc tgatcctcga
    23461 aggcgccggc tacgaccttt cccgggcgga ctacaagacc gggcgggtgc tgtccccgat
    23521 ccagatccgc ttcaaggtgg gacccgtcca gggaaaccgg tcgccggtac tggaatccgc
    23581 ggagctcgtc gtgtcttccg gggatcccga gatcgacacg gccgtcctgt tcggcttccg
    23641 ccgggcttcc ttctccaacg actccagccg cagcgtgatg ggaaccttcc tgtacaggtt
    23701 ctgatgccga gccttcagga atccctgttc agcttcccgt ccgccgaaca ggttcccatc
    23761 ctcgccctct ccgggggcgg gggcaagacc acggccctct tcggcctggc cgatgaactg
    23821 tcggcacggg gcgcccgggt cctggtgacc acgaccaccc gcatccggga cccccgggcc
    23881 gaggggcggg gtttcgaccg gttcctggag attccgggcg cgtacgctac gagcgaggcg
    23941 accgccgccg ttcccttggc ggccggactc gccgcagacc cagcggctgc ggccccgccg
    24001 ggccgcggcc cggtcacggt cctctgcgcg ggggtcgagg gcgggaagct ggtcggcctc
    24061 gacccgggga tcttcccggc cttggccgcc tcgggccgct gggacgcgat cctggtcgag
    24121 gcggacggtg cccggatgcg gccggtcaag gctccggctc cctacgaacc cgtgtggccc
    24181 gccggcacga cgtcggccct cggcgtggtc ggcctggact gcctgggccg ccccctggac
    24241 gaccgcgtcg cacaccggcc ggaactcctg ggggcgctcg tcggcctgga acccgggggt
    24301 acgatcgaac tcgggcacct cctgcgcctg gcccggcatc cggagggcct cttccggggc
    24361 gtgccggggg gagcccggcg gatcctggtc ctgaacaagg cggacctgag gccggacctg
    24421 gatccgggtg ccctcgcgga ccggacggcg gcgggcctgg cggagccggg gccggccgcc
    24481 ccgaccggat cggccctccg gcccggtccc ggtacgccgg ggatcgatct cgtcctggtc
    24541 tgttccctgg gggacccgga tccccgccgg cgggtacggg ctgcgcacaa ggttgccggg
    24601 gtgttcaaaa ggagagaacc atgaacctgt accgcatcgc cgcggacctg gaggaccgga
    24661 accggccctt cgtcctggcc acgatcgtcg aatcctcggg gtccacgccc cggagccgcg
    24721 ccaagatgat catcgaagcc gacgggacct gccacgggac catcggcggg ggccgggtgg
    24781 agtcccgggt gatcgaggag gcccgggcct gcatggcctc ggggaggccg aggaccctgg
    24841 agttcgacct gaccggatcc ggcccggacc cggatccgat gatctgcggg gggaggatga
    24901 cggtcctcct ggagcccggg gaccggaagc cccggctcct cctggtcggc gcgggacacg
    24961 tgaacctggc ggtggcccgg gcggcggccc ccctgggctt ccggatcgcc gtggtcgacg
    25021 accggccgga cctggtcacg gcggaacgct ttcccatggc ctcggaattg ttctcgggtc
    25081 cgaccctccg ggaagccctg ggggccgcgc tcccggccga ctcggccctc gccgcctcgg
    25141 ttccggccga ggggaactcc gtgctcatcg ccacggcctc ccacgcctcg gacgaggagg
    25201 ccctgcgcca ttgtatcgac aagccctgcg cctacctggg catgctgggg agccgccgaa
    25261 agacccgggt ccttctgtcc cgcctggcgg aatccggcgt ccccgaggag gccctggccc
    25321 gggtccgggc ccccgtgggc ctggacatcg gggcggaaac ccccgaggag atcgcggtct
    25381 ccatcctggg ggagatcctg gcggagcgct ccggccgggg cgggggtccc ctggcgggcc
    25441 gggagggcga cctggtcgtg gtgcgcgggg ccggggacct ggccacgggg gtcatcctgc
    25501 ggctggtccg ctcgggattt cgggtggtcg ccctggagtc gccccggccc acggccatcc
    25561 gcaggaccgt ggccttctcg gaggcggtct acgacgggtc cgcggaggtc gagggattgc
    25621 gggcccgccg tgccgagggc ctggcggaag tccgggccgc cctggcggag ggggtggtgc
    25681 cggtcgtggt ggaccccgag gccgccctgg tcccggccct ccgcccggct gtcctggtgg
    25741 acgccatcct ggccaagcgg aacctgggca cccgggtcgg ccaggccccc gcggtgatcg
    25801 ccctgggccc cggcttcgag gcgggccggg acgcggacgc ggtcgtggag accgcccgcg
    25861 ggcacgacct gggccgggtc atcctttccg gttccgcggc cccgaacacg ggggtccccg
    25921 gggaggtcgg gggcaagtcc gccgagaggg tagtccgtgc ccctgtagcc ggggaggtga
    25981 tcgcggttcg ttccatcggg gatccggtcc gggcggggga acccctcctc ctgatcgaga
    26041 ccccttcggg ccgtgtttcc gtgccctccc ccctggacgg gaccctgcgg ggcctcatac
    26101 gcccggggat cgaggcggcc gagggggaga agatcgcgga cgtggacccc cggggccggc
    26161 cggagtactg ccgcaccgtg tccgacaagg cccgggccgt ggcgggcggg gtcctggagg
    26221 ccctgttgta cctgcggggg cggacgaaat agcggccgac ggttcccccg gcttgggaat
    26281 ctcgctatac tgccattccg tatggacacc ttcgacgtag tcatcgtggg ctccgggccc
    26341 gcgggcctgg gggccgcctt cgagatctct tcccgtgacc cggggcgctc gatcctcatg
    26401 atcgacaagg agcccttctc caccgggggc ctgcggaacg actgcaagat gaacttcacc
    26461 tggcccatcg gctttccgga gaacgtctgg accgaggccg acgggctttc ctacctggcc
    26521 cgggtggagt ccttcctgaa gccggacctg atggaccggc ggaacatgga catctacgca
    26581 cggagggcct ccaagctggg ggtccggctt ctggaggtcc gccaggccca cctgggcacg
    26641 gacggggggc tggccctgat caaggacctg accgcggagc tggcccgccg gggggtgtcc
    26701 atctccctgg gggagaccgc cgggcgcgtc gaggccgagg gccggatcct ggtgacggac
    26761 aggcgggagg tcgggtaccg ggacctcatc ctggccccgg gccggggggg cttctccttc
    26821 cttcggcaca tgatgaacga cctggggatc cccttccagg acaacgccgt ggacatcggg
    26881 gtccgggtgg agctcaagga ggagcggtac gccatcgtcc gggactacta cgaccccaag
    26941 ttcctcttcc ccaagaagac ccgcaccttc tgcacgaact cccgctgcgc ccacgtggtc
    27001 caggaaaaat acgtcaccga cgacgggagg atctggtact ccgccaacgg ccacgcgtac
    27061 tccgcatccc ggccggccaa cggcctggcg aacttcgccc tcctcaagac cgtcaccctg
    27121 acccagcccc tggcctcggg ccaggactac gcctcgatgc tgggggtcca ggcctccctg
//
LOCUS       DAOTEI010000028        27060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1162_length_27060_cov_5.388928, whole genome shotgun sequence.
ACCESSION   DAOTEI010000028 DAOTEI010000000
VERSION     DAOTEI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 27060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27060
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_27060_cov_5.388928"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="P5313_04395"
     CDS             complement(<1..1137)
                     /locus_tag="P5313_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY79637.1"
                     /translation="MITFTLNGRSVSVPEGATVLDAARAAGVDIPTLCHIEGLEGYGG
                     CRVCMVEVEGSRTGCAPACTLQAAEGMTVRTDSEAAVELRRAVLEMILAEHPADCFNC
                     KHSGACRLHELAARHGLEKVPSGIERSGFPVDDSNPFFTLDPNRCILCGKCVRTCAEI
                     QCSDVYTFADRGCRSSVAPGFGDLLSDSRCVSCGSCVAACPTGALRPKDGSWAGAMYV
                     DRRVRTVCSYCGVGCQLDLLVAGGKVVGALPADGPSNRGRLCVKGRFAHSFIGHPDRL
                     TSPLVRRDGKLVEAAWDEALDLVAGRFRAALDRLGPHGVAGLASARCTNEENYLFQKL
                     FRAVAGTNNVDHCARLCHASTVAGLAATLGSGAMTNSIDEIDVQD"
     gene            1320..3656
                     /locus_tag="P5313_04400"
     CDS             1320..3656
                     /locus_tag="P5313_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY79638.1"
                     /translation="MPANSGTILLVEDEALIALAESRTLEKEGYAVLRAGSGEEAVEI
                     ASANPGIDLVLMDVDLGPGMDGTRAAREILETRDIPVLFLSSHVEPEVVARTEAVTSY
                     GYVVKNSGTMVLLASIKMAFRLHESNRSAALSARRFEAVSGTTMEGFWISDSEGRILE
                     VNQAYSRMTGYSREELLGLRISDLEAVMDETAIGEEILRIRRLGSDRFETRHRTKDGR
                     TVEVEVNATDVPDQGFTICFLRDITELKRAEDLRREREQYSQLLSRMTAEMLDQGTPE
                     DLYRYIVRRMEERHPDTVVLFISIDEKEGLTRLECVAGLERNLLRRALDLIGFDPVGR
                     TYPLVTSHDEYFRSGRLVCFEGGLAEFCAGQFPEPAARAVETLLGLERIYTIGLAKGA
                     VLLGALHFLTFRGTEIPDPEFVEAFVRQAGLVLRQKIAEAEARESEGRIRKLSDNLPG
                     GIVYQVDFGSEGRDRRFTYISGGVETLHGVSAEAVLENPSSLYGQFPEEERRKIADLE
                     EEALRSMSRFYVEARFRKPDGTERWGLLSSMPRKLPNGHVLWDGIEIDITERKRAEEA
                     AERHLREKELVLREVHHRVKNNLGAVESMLRLQADLREDPGLRSVLQDAAGRVVSMEV
                     LYDRLYRAGDFGELPVREYLETLLPEIIRCHARSENVRTVVEAGDFRLGAKTLSALGI
                     LLYELVNNALKHAFEGRAGGTIRVTASRAGDRVRVVLADDGIGLPPSVTLENSSGFGM
                     RLVGMLADQLGGGIRVERDGGTRFTLEFPEIKTERGES"
     gene            complement(3702..4679)
                     /locus_tag="P5313_04405"
     CDS             complement(3702..4679)
                     /locus_tag="P5313_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79639.1"
                     /translation="MDFHAGRVRITVARDEADMGNRAAAEAARAAKDAVASGKKVAFW
                     LMAAPSGFSFYEAFVARTREDPGLAAAARSARYFQFDDYPIGRGDPRFPVTFRHLLES
                     RFFGPLENAAGPLEGKRLLELKGDEGDEAVLEGYRESLQEVLEDEGWYVIEVKGIGMD
                     GHWGFHGRETPLDSPPGFIRVPMSPLNVQQQLLDWPRYFRSAADVPEAAVTANVGLFL
                     KADRILDLVPQRTKEFAVLACYGPDAVSPLVPSSALKTHPAARSFLTELSAEALLEYR
                     RRGAPESPVSEETLERLKGAWRSPGDPERERRNIAEMLETLKAVFGQAG"
     gene            complement(4700..5842)
                     /locus_tag="P5313_04410"
     CDS             complement(4700..5842)
                     /locus_tag="P5313_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY79640.1"
                     /translation="MRVQGVDVVSGKALSLEIRDGFVAGIERIPPEPGMPWLSRGFLD
                     IQVNGYRGIDYSGAGLEGADVTRMAGFLAVSGTTRHVPTIITNSRERILRNLEIIAGA
                     VSSDPGLAAAIPGIHLEGPFICRDEGPRGAHDPRYVRDPSLAEFEEWIAASRGLLRMV
                     TLAPELDGALELIEEGVRRGVVMAIGHTAASGDRIARAVAAGASLSTHLGNGSHATVP
                     RLRNYLWEQLGADGLAASVIADGFHLPESVLKVFARAKGLERLVLVSDVASLGGSEPG
                     AYRWGDVEVEVYPDRHIGLRGTPFLAGAGHLLDWDIAHFARATGTDIRDAVRLATSNP
                     EAVLGLPSPGPGFRVGDRADLTRFTYDASGGPLVILDTVFGTEIRR"
     gene            6002..7444
                     /locus_tag="P5313_04415"
                     /pseudo
     CDS             6002..7444
                     /locus_tag="P5313_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
     gene            7624..8172
                     /locus_tag="P5313_04420"
     CDS             7624..8172
                     /locus_tag="P5313_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79641.1"
                     /translation="MKKYVYLVLALGLVLIAASCSSMAQGALSGALGAVVPGGGGGSS
                     AVASAAAPVEFQSGEILASADSRSMFDSGFRLAKILTPASAATKNQAEALWVSDGKKQ
                     WVNYVIGSRKATKADFVIGAPIFYLNGWANHDKISADDYRKDGWALGNITSIELLYKN
                     QVEIDGQPYYIEYLRVPTDPVK"
     gene            complement(8315..9493)
                     /locus_tag="P5313_04425"
     CDS             complement(8315..9493)
                     /locus_tag="P5313_04425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY79642.1"
                     /translation="MEEHSGPKDTRGTAGALDLYKALAESGTWESLEALRRENRELES
                     LVNDVSGLLGLSGIPEMMEFVISRFLDRFIPVFLAFLIEPPRGGRVRQYCYRSLHPAD
                     EEVPIRYYRALKRLFLEDSRSRTFEEARQALGDSAFDRDFLSYEPELILPMRGIGGLY
                     GIILLGRKIAGAEYTLPERMYVDRLSRFLSVAIQNGLHHESSITDPKTGLFNHDYFMR
                     RAEDELARSARHGSRAGMILADVDHFKRFNDRYGHLAGDEVLQVLSRVMKAATRSEDT
                     VARFGGEEFSVLIVECDEAHLAEVAERIRAAVEGMRVPYKGVVLSVTVSLGARLLDAR
                     ERRGARDLLDDADRALYASKAAGRNRVTIYRPGLLGRASMRRLPGGAAGGSAAAPAGP
                     "
     gene            9606..10088
                     /locus_tag="P5313_04430"
     CDS             9606..10088
                     /locus_tag="P5313_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRY79643.1"
                     /translation="MDNRTEYAVSEIGRTLIGRLLPGTDLVPGILSICAESGIRFGYV
                     ASLIGSLAMVRFIYVNKTPGKDSGFVFSKPVEKAGPLEILSGQGTLGEGEDGKPFLHL
                     HALLCDEKDRISGGHILETGCPVLATIEVVIRETRGIRLVRGPDEETGMSLFRVSSGF
                     "
     gene            complement(10129..11169)
                     /locus_tag="P5313_04435"
     CDS             complement(10129..11169)
                     /locus_tag="P5313_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY79644.1"
                     /translation="MEKAGLAEQELRHERERLQFIIDGAGLGTWEWDVRTNRTVFNET
                     WAAMLGYTLAELTPYDYSTWVRLVHPDDLVHAEAALDECISGRAPSYECEFRMLNKQG
                     RWVWILDQGRVMTRDPEGRPSLMFGTHTNIDRIKRTEEDLRRGLEEKDLLLRETHHRI
                     KNNLASIASLLSLQSGYVESPEVHSALNEALGRVRSMQALYTKMLSAGQDVRAVSVPG
                     YLADIADSVTGLFPSTAEVSVEKRLAPLSLDAKKLLPLGIILNELLTNAMKYAFQGRK
                     TGRIEISLELREGRAVFSVGDDGTGLPPDLDPAVSPGFGLTLVRIISEQLGGSARFET
                     GVGTRVTVEFPV"
     gene            11361..12026
                     /locus_tag="P5313_04440"
     CDS             11361..12026
                     /locus_tag="P5313_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79645.1"
                     /translation="MQETDGPDTRTRILDAAEEIAREQGLDSLSMRALAERIGLSAPA
                     AYRHFRSKSEIVRAMIDRGRDRFARGLAAARRGTEGPEEILAASARYYLRFWLRDRAG
                     FRLASEWGRQEPSLTGRAVEAGSFGDVPALVAAILGETRDPGETARVSRWVASTLYGM
                     ALSLVQDEGIPRAREMELVDSAADYLVGAVRAAASGRLSGGGSPAGNRGTARRNGRGR
                     SDP"
     gene            12023..12421
                     /locus_tag="P5313_04445"
     CDS             12023..12421
                     /locus_tag="P5313_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRY79646.1"
                     /translation="MRSEKRRRAVAAAAALALTLGSCAFMGEIDRIEIVEPDLAKAAD
                     GTWVGEWNTALVSATVSVDTLSHGISGIDILRHECGKGRPAEAIVDRVVAAQRLTVDT
                     VSGATGSSKVILRAIQDALEKAVAAGEATP"
     gene            12418..13359
                     /locus_tag="P5313_04450"
     CDS             12418..13359
                     /locus_tag="P5313_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79647.1"
                     /translation="MNIAVRILITAVAVAAALFLAARLGGLERRARGDVEGARSLPPV
                     FDYAAGPAAGNPGPDAVPAGDGWVDMATAAPGTPVTDADIDALPEPARRYFRHANVAG
                     KPRISSFSVVLEGRIRNGPAESWMPLVMRQFNRLDNPARVVYLTSTKPPMNGIDSFLD
                     GQGRMLIKAADLVKVVDSRGPEMAVSAFVTFLNDLVLCPLAYFSLPLRWRPAGPDSVE
                     LSMTHAGMTVSAVMTVDAEGRPVGWRSEDRYAEVKGRNRKDRWSTPFEGSKEIAGLRI
                     PEKGAGIHDYDGTPYVYVELERIRSLVLDAEGLPGRP"
     gene            13449..14624
                     /locus_tag="P5313_04455"
     CDS             13449..14624
                     /locus_tag="P5313_04455"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRY79648.1"
                     /translation="MRNPSAYTPGSSRRPASRTARACLPAIAFSAVLLAACGPGRAAP
                     PRDPAALRARAAELSARLLIADGHIDYPDRASPYEDLTAGTSGNFDEPRARKGGLNLA
                     FLAAYVPPEDEDAGTAFSRAEERIDLVLKAADIHPDKLALARSPEEARVAFGAGRLAL
                     AVGIENGAPLEGRLDRVGYFRDRGVAYIGLVHSEDNHLCDSSMEPDPEHRWNGLSSFG
                     REVVREMNRLGLMVDVSHATDDAIRQILELSTAPVIASHSGLRSLTPGFERNLPDDLV
                     RGVAESGGVVCLAFGSYFLRDAFRTTHAAADLATARDVAEAIDRTVRIVGVDHVGLGS
                     DFDGVGFSLPKDLADVSMYVNLVRELLSMGYGEEDLAKILGGNLLRVWGEVRAKAEE"
     gene            complement(14676..16553)
                     /locus_tag="P5313_04460"
     CDS             complement(14676..16553)
                     /locus_tag="P5313_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY79649.1"
                     /translation="MEAVQRVLFVDPSTGFYRLRRYKVGDYFGPVDLGLHITDRWRSL
                     NIGVGIFAGSILPGSNRLIVNGFSPCWRGFYVSSMGGAGVAFDNLGINLISIVGKAPV
                     PSILYLNRSHGEEIDVEVIPVDLAEAWSHGRGGLYGLMELVLDKYGNRYETDPRVLAT
                     GPAARSTDFGAVGSAPVVKGKLTYVDTWAGRGGFGSNMLQEHGLAAVVFGGTFLDEDF
                     RDRKVADEWFEQKYKQKLAAKDFESTTKYRFDPKFETGGTFGVNYATLKGRVMAFNYR
                     TIFDSEDARLDLHKRLILDHYLKQFNEETIQPRKQATCGEPCAAVCKKMNGEFKKDYE
                     PYQTMGPLCGVFDQRAAERLNHKADAAGFDGISLGGVLAWFMDLLDRGELAGADLGVS
                     GEPKFDAAGFDPVADSAANADLAEGLIDTILEKRGIVDLSEGVRKFARKAAKERGVRI
                     LNSFVHTANGRRGWMVPNQYWTPGALAPMAIMGKYYMHYGPEFLPPRKLGRTCAERLK
                     KELVMDNLGVCRFHRGWSEEMLPEIMESLYGNRDRFLAATAVCASRINSRNASVYWES
                     ERNLDFVVSFLKRRRDVEGDADPELARWIDEFDKDRKEAGLSFWYEMKKGIDESLREF
                     F"
     gene            complement(16663..18588)
                     /locus_tag="P5313_04465"
     CDS             complement(16663..18588)
                     /locus_tag="P5313_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY79650.1"
                     /translation="MDSAGNRYRMEVSGFLIRAGLVLGTVLLFPSLLYAIRSGKTALV
                     VADGSAYAAFLSLYLLNRWKPRLSAGGLVGLVFGFGCYLLAANGPAAAGTIWMGAAVV
                     LAAVLFGTAGGVLGAALVGAAFLAVTFAASRGLPTWSAPSAFLVILGLNAVVMSLAAG
                     LAASYVARSLERALAERERLSRQLAERSAALEREAAERLGAERKAEFYRDFDGRTGLP
                     NRERFLRELERTLVPASRRGRPLSVLAVGIHRPGLIFDEFGHVQGEAAIAGAAARLRE
                     AFREDDVVARFGDALFFVLCSDLGSPDDIAELLPKAFSAFERPVEAGSHLIHVAAVVG
                     VALYPHDGDTPDELVRAATAAYHHAEEDGPGAYRVFDASLHADLIERLRFERDLAGAV
                     RDGALVPWFQPKVDGRGAVVGAEALARWPRPDGSLGMPSDFIPVAERAGLMEALGRDI
                     LRKSCRRAREWNAGASGPASVAVNLSPQALRKPAIAEEVWKALEETGLDPALLELEIT
                     ETGLALAAEAAVPRLRELKSLGVSLAIDDFGTGASGISRLRDFPVDFVKLPLDLVEPL
                     PSDRRSRAIVKAVIDLAHALGFRVVAEGVESAGQLEWLGEASCDLFQGFLFAPPLPAD
                     RFRSALGEGYSKVLGNG"
     gene            complement(18783..18856)
                     /locus_tag="P5313_04470"
     tRNA            complement(18783..18856)
                     /locus_tag="P5313_04470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18820..18822),aa:Val,seq:gac)
     gene            complement(18974..19411)
                     /locus_tag="P5313_04475"
     CDS             complement(18974..19411)
                     /locus_tag="P5313_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79651.1"
                     /translation="MKTKEDFPEINARRPDGLKPEGTPYRVLIVDDSMFVAKQLNQIL
                     TSEGYQVVATAGDGLEGYEKYKELYPNLDVVTMDITMPKMDGLSALQKIMEFDKNAKV
                     IMISALGKQDLVKQALVAGAKNYIVKPLDRKKVLERFLSVVGK"
     gene            complement(19430..19894)
                     /locus_tag="P5313_04480"
     CDS             complement(19430..19894)
                     /locus_tag="P5313_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRY79652.1"
                     /translation="MRVEYINPFVESAFNVLKEVLQGEVKRGELYLKSTSIPVMGVAA
                     IVGLAGDVEGRVLFDMDKPTALKVASVMNGEELKVMDELVKATITELANMITAQAVTK
                     LHELGFKFDLTPPAIFTGENMEVSDQDVEALIVPMETAQGKLEINVAIRERG"
     gene            complement(19916..21292)
                     /locus_tag="P5313_04485"
     CDS             complement(19916..21292)
                     /locus_tag="P5313_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY79653.1"
                     /translation="MSSQTNPLDTMILERTEETRKERAEKVDFKMVTFSLGGKEYGID
                     IMNVKEIARAGRFTYVPNAAPFVRGVYNLRGDIISVIDLRVMFNLPVEKREADAVESL
                     LILRVEDHVFGVIVDSIDKVVGISRASIQPPHPIFGDINVKYIRGIVENAGKLYIILD
                     IAKIFSPKAEKEEHPAHYEAPRVEAAPAAAAGTRSASARSAPERSQTERSAIVDAEMT
                     SGFVKEGLFSFRHFAVSAVNDAWFRARYAEWERLRKGTDLQLHEDGDADAFLEGFLSP
                     YTGTLWGADYLDAVASILPDTDVRSINVWNPGCGKGFETWSLACALRRKYPQARVKIW
                     ANDSDLLAISMAPNMVFDAASAPEPYRTYLVKGMNGWTFDQAVRDSIFFEFHDILNAN
                     PLPPVDLILCRDMLSFLSPEDQGRVLADFQEKLKGAGVLILGTNETAATGAWRPVGSG
                     AVSAFAKA"
     gene            complement(21312..23810)
                     /locus_tag="P5313_04490"
     CDS             complement(21312..23810)
                     /locus_tag="P5313_04490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRY79654.1"
                     /translation="MSDYLDPNNEELLKDFFAEAQSQVDALEQNILVLENDPGNRDAV
                     DEIFRAAHTLKGGAATVQMMELQGFTHLVEDVLDEIRSDRLKVTEEAVDVLLKAIDVV
                     KAMLEARMDGSVFDGDVSPVREALSGFLPGEGSKAKGGAKAAASKPGKAKPAPAPEPE
                     ARDVPAQSVSAAGLTEYEILELKEAAGKDRRVYRVTVVFDESNLMKTISAIHAFAALR
                     DAGTVLKTHPDFEKLYEDQFFPEVDYYLATDRTAEDLDRKARIPEVTKDVRVTELTFE
                     EGGRPAPAPAATKPAPAKSSVPRAEAPSPGAVTEDELARAVARKKEAAAAPAASAAAP
                     ASAPAKGAVPEPSAPAAGAEEHPEEAAEEGDRRAAPKVQQGSILRVDSRRIDNLLNLV
                     SETVINKATFNQISSQFSELQGTFLAVQSEFRDRLKELFDEVNEALVSGKSVKDMKKA
                     LQERWAVLAAAHDPFEAEFKSTVAKFRSTAQNLGRITGELQEGVMRIRMVPISQIFSR
                     FPRLVRDLSKSLSKQVDLVIEGEDTELDKSVIEDLLDPLMHSVRNALDHGVESPDIRR
                     AAGKPETGTVHLRASNEGNMIIIEIEDDGKGIDVEAVKRKAVDRGLIHPNKVLTDVEA
                     FNLIFEAGFSTAAAITNISGRGVGLDVVRRQIEKLNGSVAVTSRPGKGTKFTIKLPLT
                     LAIIQGLLVRVGRETYSIPITSVIESHRIKPEEIRMIDNYEVFNVRSDVISLLRLDRL
                     FRIPTSGQREYNFVVIVGTNEKRVGLMVDSLIGEEDVVIKPLRDQYTASPGIAGASIL
                     GDGSVCLIIDVSQLLELGLKREKEERARLEGAIR"
     gene            23950..>24754
                     /locus_tag="P5313_04495"
     CDS             23950..>24754
                     /locus_tag="P5313_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79655.1"
                     /translation="MRTSRAAAAAALILALAAAPLSGLEIATEARAGNIFFPWPQAAA
                     TTGAFPSDNYFWGAALSVSDSVGDSLSYRIGYETDPVLRHLVTVLLTYDLGFAQFSAG
                     PFLGAFNSREAPLKGGLSTSLRFEWPGRVFASIRSDSSLGGGLLAAGDYIQETTEISA
                     GWYVYNAICTLSMLTKKFYIQETDTLMTQDRLARYTFDVNVYRKGAPLTLVWTLGYQA
                     LSKTWDDGTPETDTLGSVILGTRADWRVARKLRVTARLETSVFSFGADGL"
     assembly_gap    24755..24764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24882..26006)
                     /gene="ricT"
                     /locus_tag="P5313_04500"
     CDS             complement(24882..26006)
                     /gene="ricT"
                     /locus_tag="P5313_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRY79656.1"
                     /translation="MTRYADHDMTEEELRHLDDEGADVERKDSRPSQSLPWPLYRLAI
                     EHSHETFLAVYDGPVPQPPELSPAVPVPEVPDLTAPPVAEAAGSAEGRDAPESPPESP
                     AAQVSGTPAESAEPEAPAPAPRILAVGSYVLVSTRYGRDLCRVMGLIRNPGYTSPDEI
                     ARIERPATPSDLERKAQNAEKERHAFTVCQDRIAARRLPMKLVSSHYLLEEPKVLFFF
                     TADSRVDFRELVKDLVSVFKMRIELRQIGVRDEARVTGGCGVCGRVLCCHGVTDKLVP
                     VSIKMAKDQNLSLNSMKISGPCGRLLCCLAYEFGFYREARRSIPPEGARFPWDGVVFR
                     VTEINVPAGKVRISGEDGRVLVVDASRFSQAEGRWALKNE"
     gene            complement(26023..26526)
                     /locus_tag="P5313_04505"
     CDS             complement(26023..26526)
                     /locus_tag="P5313_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HRY79657.1"
                     /translation="MAKIDLPDNLLSGLVPGLSGRKPREERKVDKGKGVFRTLLRDAE
                     RTEDGFHAESVAEAPFSQADLEALLDDVHGLGEDLKRDPNPDAVVAYKLAVRNFVHFV
                     VSRAYTLEEKTSGTNILKRKKYVILSVVDQKLERLAAEILSTQRNQLEILRKVDEIYG
                     ILVDLLQ"
     gene            complement(26514..26969)
                     /locus_tag="P5313_04510"
     CDS             complement(26514..26969)
                     /locus_tag="P5313_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY79658.1"
                     /translation="MANLRDSLVNSLVGTGSSLRGDLVVDGLLRVDGDVTGAIRSTGK
                     VVIGQGGRCESSIQARSAIIGGLVKGDVCVTERLSVLDGAVIVGNVFAPRLDAEGDIV
                     IHGDVEVSGKVEKAEDALLDFQARHGTRVPAADPVDPRRGAGTEGPWRK"
BASE COUNT         4294 a         9285 c         9101 g         4370 t
ORIGIN      
        1 gtcctggacg tcgatctcgt cgatcgagtt ggtcatggct ccggagccca gggtggcggc
       61 cagacccgcc acggtggagg cgtggcagag acgggcgcag tggtccacgt tgttggtccc
      121 cgcgaccgcc cggaagagtt tctggaaaag gtagttctcc tcgttcgtgc accgcgccga
      181 ggccaagccc gcgacaccgt gaggacccag gcggtccagg gcggcccgga agcggccggc
      241 caccaggtcc agggcctcgt cccaggcagc ctcgaccagc ttaccgtccc ggcggaccag
      301 gggagaggtg aggcggtccg gatgcccgat gaaactgtgg gcgaaacggc ccttcacgca
      361 caggcgtccg cggttggacg gtccgtccgc gggcagggcc cccacgacct tgccgcccgc
      421 gaccaggagg tccagctggc agcccacccc gcagtaggag cagacggtcc ggaccctgcg
      481 gtccacgtac atcgccccgg cccaggatcc gtccttgggc cgcagggctc cggtcgggca
      541 ggcggccacg caggacccgc aggagacgca gcggctgtcc gaaaggaggt ccccgaaacc
      601 cggggccacg ctggaccggc agccccggtc cgcgaaggta tacacgtcgg agcactggat
      661 ctcggcgcag gtccggacgc acttgccgca gaggatgcag cggttggggt ccagggtgaa
      721 gaaggggttg gagtcgtcga cggggaagcc gctccgctcg atcccgctgg ggaccttctc
      781 gagcccgtgc cgggccgcca gctcgtggag tcggcaggcc ccgctgtgct tgcagttgaa
      841 gcagtccgcg ggatgttccg ccaggatcat ctcgagcacc gccctccgga gctcgacggc
      901 cgcctcggaa tccgtgcgga ccgtcatgcc ctccgcggcc tggagggtac aggcgggggc
      961 gcagcccgtc cgggagccct ccacctccac catgcagacc cggcagccgc cgtagccctc
     1021 gagcccctcg atgtggcaga gggtggggat gtcgactccc gcggcccggg ccgcatccag
     1081 cacggtcgcg ccctcgggga cggatacgga ccttccgttc agggtgaagg tgatcataga
     1141 ggaagcagta tgtatggaaa tcttggatat tacaaggaga aaccggaccg ccgccggggc
     1201 ggcgcggcct ccctgcggct tccagtgttg cgccggcgcc cggctcggcc tacaatgaaa
     1261 gagcggttcg gtcagatttg gtgaggagcc ccgccggttc acgacgggca gggagaccca
     1321 tgccggcaaa ttccggaacc atcctcctcg tggaggatga agccctcata gccctggccg
     1381 agtcccgtac cctggaaaag gaaggatatg cggtcctccg ggccggttcc ggagaagagg
     1441 cggtggagat cgcctcggcg aacccgggca tcgacctggt gctcatggac gtggacctgg
     1501 gtcccggcat ggacggaacc cgtgcggccc gagaaatcct ggaaacccgg gatatccccg
     1561 tcctgttcct ctcctcccac gtggagcccg aggtcgtcgc ccgcaccgag gccgtcacct
     1621 cctacgggta cgtggtcaag aactccggaa ccatggtcct gctcgcctcc atcaagatgg
     1681 ccttccgcct gcacgagtcc aaccgctcgg ccgccctcag cgcccgccgc ttcgaggccg
     1741 tgtcgggcac caccatggag ggattctgga tctccgactc cgagggccga atcctggagg
     1801 tcaaccaggc ctattcccgg atgacgggct acagccgcga ggaactgctg ggcctgcgca
     1861 tatccgacct ggaggccgtc atggacgaga cggccatcgg ggaagagatc ctccggatcc
     1921 ggcggctcgg gtcggaccgc ttcgagaccc ggcaccggac caaggacggc cggacggtcg
     1981 aggtcgaggt gaacgccacg gacgtgcccg accaggggtt cacgatctgc tttctccggg
     2041 acatcacgga gctgaagcgg gccgaggacc tccggaggga acgggagcag tattcccagc
     2101 tcctcagccg gatgaccgcg gagatgctcg accaggggac tcccgaggac ctctatcgct
     2161 acatcgtccg ccgcatggag gaacggcatc cggacacggt ggtcctgttc atctcgatcg
     2221 acgagaagga aggcctgacc cggctggaat gcgtggccgg cctggagcgg aatctcctgc
     2281 gtcgggccct ggacctgatc ggtttcgatc cggtcgggcg gacctaccct ttggtcacat
     2341 cccacgacga gtatttccgg tccggccgac tcgtgtgctt cgagggaggg ctggcggagt
     2401 tctgcgcggg ccagttcccg gagccggccg ctcgggccgt ggaaaccctc ctggggctag
     2461 aacggatcta cacgatcggc ctggccaagg gcgccgtcct cctgggcgcc ctgcacttcc
     2521 taaccttccg gggtacggag atcccggatc cggaattcgt cgaggccttc gtgcgtcagg
     2581 cggggctggt ccttcgccag aagatcgcgg aggcggaggc ccgggagtcc gagggccgca
     2641 tccggaagct ttccgacaac ctgcccggcg ggatcgtcta ccaggtggat ttcgggtccg
     2701 agggccggga caggcgcttc acctacatca gcggcggggt ggagaccctg cacggagtgt
     2761 ccgcggaagc cgtcctggaa aatccctcgt ccctgtacgg ccagttcccc gaggaggagc
     2821 gccggaagat cgcggacctg gaggaggagg cgctccggag catgtcgagg ttctacgtgg
     2881 aggcccgctt ccggaagccc gacggcacgg agcgttgggg gctcctctcg tccatgcctc
     2941 ggaagctgcc gaacggccac gtgctctggg acgggatcga gatcgacatc accgaacgca
     3001 agcgcgccga ggaggccgcg gaacggcacc tgcgggagaa ggagcttgtc ctcagggagg
     3061 tccaccaccg ggtcaagaac aacctggggg ccgtggagag catgctccgg ctccaggcgg
     3121 acctccggga ggaccccgga ctacgctccg tcctccagga cgccgcgggc agggtggtga
     3181 gcatggaggt cctctacgac cggctgtacc gggccggaga cttcggggag ctccccgtcc
     3241 gggagtacct ggaaaccctc ctgcccgaga tcatccggtg ccacgcccgg tcggagaacg
     3301 tccggaccgt ggtggaagcc ggggacttcc ggctgggcgc gaagaccctc tccgccctgg
     3361 gtatcctcct ctacgaactg gtcaacaacg ccctgaagca cgccttcgag ggccgggcgg
     3421 ggggcacgat ccgggttacg gcctcccggg ccggggatcg ggtccgggtc gtcctggcgg
     3481 acgacgggat cgggctaccg ccctcggtga cgctggagaa ttcctcgggc ttcggcatgc
     3541 ggctggtcgg gatgctggcg gaccaactgg ggggcggaat ccgggtggag cgggacgggg
     3601 ggacgcggtt caccctggag ttcccggaga tcaagaccga gcgcggcgag agttgagcgc
     3661 ggttcccgga caggcggctc gggccgcccg ggatccgcgg ttcaccccgc ttgcccgaac
     3721 acggccttca acgtttccag catctccgcg atgttccgcc gctcccgttc gggatccccg
     3781 ggacttctcc aggccccctt caggcgttcg agggtttcct cggacacggg gctctccgga
     3841 gcgccccgac ggcgatactc caggagcgct tccgcggaaa gctccgtcag gaaggaacgg
     3901 gctgcggggt gcgtcttgag agccgaggac gggacgaggg ggctcaccgc gtccggtccg
     3961 tagcaggcca ggacggcgaa ttccttggtc cgctggggca ccaggtccag gatgcggtcc
     4021 gccttcagga agagccccac gttcgcggtg accgcggcct cgggcacgtc ggcggcggag
     4081 cggaagtacc gcggccagtc cagcagctgc tgctgcacgt tcagggggga catcggcacc
     4141 cggatgaagc ccgggggcga gtccaggggg gtttccctcc cgtggaaacc ccagtgcccg
     4201 tccatgccga tgcccttgac ctcgatcacg taccagccct cgtcctccag gacctcctgg
     4261 agggattcgc ggtacccctc caggacggct tcgtcccctt cgtccccctt caattccagt
     4321 aggcgtttgc cttccagggg tcccgctgcg ttctcgaggg gtccgaagaa ccgggactcc
     4381 aggaggtgcc ggaaggtgac ggggaaccgg ggatccccgc ggccgatcgg gtaatcgtcg
     4441 aactggaagt accgggccga gcgggccgcg gcggccaggc cggggtcctc ccgcgtccgg
     4501 gcgacgaagg cttcgtaaaa cgaaaagccg ctgggcgccg ccatcagcca gaacgccacc
     4561 ttcttgcccg acgcgacggc gtccttcgcc gcccgggcgg cctccgcggc cgcccggttg
     4621 cccatgtccg cctcgtcccg ggccacggtt atccggaccc tgccggcatg gaagtccata
     4681 cccgtctcct tccgatccgt catcgccgga tctccgtccc gaacacggtg tccaggatga
     4741 ccagggggcc ccccgaggcg tcgtaggtga accgcgtgag atcggcccgg tccccgaccc
     4801 ggaacccggg ccccggggag ggcaggccca ggaccgcctc gggattcgag gtggccagac
     4861 ggaccgcgtc ccggatgtcc gtgcccgtgg cccgggcgaa gtgggcgatg tcccagtcga
     4921 gcaggtggcc cgccccggcc aggaagggcg tcccccgcag gccgatgtgc cggtccgggt
     4981 acacctccac ctcgacgtcc ccccaccgat aggcgccggg ctcgctgccg cccagggagg
     5041 ccacgtcgct gacgagcacg agccgctcca gtcccttggc ccgggcgaag accttgagga
     5101 cgctttccgg caggtggaag ccgtccgcga tgacgctggc cgcgagcccg tccgcgccca
     5161 gctgttccca gaggtagttg cggagcctgg gcaccgtcgc gtggctgccg ttgcccaggt
     5221 gggtggagag cgaggccccg gccgcgacgg cccgggcgat ccggtccccc gaggccgcgg
     5281 tatggccgat ggccatgacg actcccctcc ggaccccctc ctcgatcagc tccagggccc
     5341 cgtccagttc cggagccagg gtcaccatcc ggagcaggcc ccgcgacgcg gcgatccatt
     5401 cctcgaactc cgccaaggaa ggatcccgga cgtaccgggg gtcgtgggct cccctcggtc
     5461 cctcgtcgcg gcagatgaag ggcccctcca ggtggattcc cgggatcgcc gccgcgaggc
     5521 cgggatccga ggataccgcg ccggcgatga tctcgaggtt gcgcaggatc cgctcccggc
     5581 tgttcgtgat gatcgtgggc acgtgcctcg tggtgcccga gaccgccagg aagccggcca
     5641 tccgtgtgac gtccgcccct tcgagtccgg cgccgctgta gtcgatgccc cggtacccgt
     5701 tcacctggat gtccaggaac ccccggctca gccagggcat gcccggctcc ggcggaatcc
     5761 gttcgatccc ggccacgaag ccgtcccgga tctccaggga aagggccttc ccggagacga
     5821 catccacccc ttgtaccctc atctcccctc tcctgtcatg aagacggcct ccgacaagat
     5881 gaaaccatcc gcgttccggg ttcgtcaacc gaggcccggc accccaccgt tccgtccgaa
     5941 acttgccgtc cccggaatcg tgcactatct tttgcgcaat cccggatcgc gaggtgagcc
     6001 gttgaaagcc cggaaaagcg tcgaaccctc ctcggccgcc ccccggttct acgtggccgt
     6061 gggggcctcc gcgggcggcc tcgaggcgat cgacgagttc ttcacgaaca tgcccgcgga
     6121 cacggggttg gccttcatcg tgatccagca cctgtcgccg gattacaaga gcctgatggt
     6181 ggagctcctg tccaagaaga ccgctatgca ggtcctgcgc gccgaggacg gccttgcggt
     6241 cgaacccgac cgggtctacc tgatcccgcc ccggaagaac ctcagcatct tccacgggaa
     6301 gctcctgctg agcgagcagg accacgcccg gggcgtcaac cttcccatcg acatcttcct
     6361 gagctccctg gcggaggacc agggggaaaa ggccgtggcg gtgatcctct ccggcacggg
     6421 atccgacggg atgcggggag tgcggaaggt caaggaaagc ggcggcatgg tgatggtcca
     6481 ggacgaggac agcgccaagt tcaacggcat gccccgggcc gccatctcca cgggcctgac
     6541 ggacttcatc ctgaaaccct cggacatgcc ccgccagctc ctcgggttca tcgaccggcc
     6601 ctacgcgtcc ctggccgcgc gctcggactc cctgctgtcc gacgaggacg gcctgacccg
     6661 catcttcacc ctgctgcggg aacgctgcaa ggtggacttc accttctaca agccgagcac
     6721 cgtgacccgt cgaatcgaac gccgtatgac ggtcacccag tcggacacca tcggggacta
     6781 cgtcgcccac ctgaacgcaa accccctgga gatctccgcc ctcttccagg agctcctgat
     6841 cggggtgacg agcttgttcc gggatcccgc cgcctgggag cgattggcct ccaaggaact
     6901 gccggagctg atccgcgccg ccgagggccg ggaactccgg ttctgggtgg ccgcctgttc
     6961 cacgggggag gaggcctaca gcctggccat cctggcccgg gaggtcatgg aggagctggg
     7021 ggtccgtcgg gacgtgaaaa tcttcgcaac ggacatcgac aaggacgcca tccacttcgc
     7081 cgcggcgggg gtgtacccgg aaagcgtggc cgcggacctc tccccccgat acctggccaa
     7141 gtacttcttc aagcgggagg aatccttcca ggtatcccgg gcggtccggg agatggtggt
     7201 cttcgcccag cacaacctga tcaaggatcc gcccttcacg aacatcgacc tcgtgagctg
     7261 ccggaacgtc ctcatctacc tccagcccaa cctccagcgc aaggtcctgg attacttcac
     7321 cttctccctc cttcccggag gggtcctctt cctggggacc agcgagaccg ccggggaggc
     7381 ggcggagtac ttcgaactgg cggacgcgaa gaacaaggtc taccggtccg ccggccggtc
     7441 ccggcccctc ctggaacctg tccagaaccc ttcgatcacg gacacccggg ccgcggcgca
     7501 aacccggatc cgggacctgg agtccggaaa cccggccggc tcttcatgtc cttgatttcc
     7561 tgggttacgg gtatatacta atccccggca ccccgagggg atgccgtcaa aggagatagg
     7621 tccatgaaaa agtacgtcta cctcgtgctg gcgctgggat tggtcttgat cgcggcttcc
     7681 tgttcatcca tggcccaggg agccctgagc ggcgccctcg gcgcggtcgt tcccggaggc
     7741 ggcgggggga gctcggcggt agcctccgcg gcggcgcccg tcgagttcca gtccggcgag
     7801 atcctggctt ccgcggactc ccgttccatg ttcgattccg ggttccgcct cgcgaaaatc
     7861 ctgacccccg ccagcgcggc caccaagaac caggcggagg ccctctgggt ctcggacggc
     7921 aagaagcagt gggtcaacta cgtgatcggg agccggaagg cgaccaaggc ggacttcgtg
     7981 atcggcgccc cgatcttcta cctcaacggc tgggcgaacc atgacaagat ctccgcggac
     8041 gactaccgca aggacggctg ggcgctcggg aacatcacct caattgagct tctctacaag
     8101 aaccaggtcg agatcgacgg tcagccctat tatatcgagt acctccgggt acccacggat
     8161 cccgtaaagt aacggaccct tcggacccgg gggttcctta gcctgaccgg cccgggactc
     8221 ccgggccggc tccgggctcc ccggcccatc tccccctacc cgcgatcatc ctagtgcatc
     8281 ccgtgcatac ccccggccgc ggccccgtac cgttctacgg acctgcggga gccgccgccg
     8341 atccgcccgc cgcccctccc gggagcctcc gcatggaggc gcgtcccagc aggccggggc
     8401 ggtagatcgt cacccggttc cgccccgcgg ccttggacgc gtagagggcc ctgtcggcgt
     8461 cgtccaggag atcgcgggcc ccgcgccgtt cccgggcgtc cagcaggcgg gcgccgaggc
     8521 tcacggtgac ggacaggacc acccccttgt aggggacccg catgccctcc acggcggccc
     8581 ggatccgctc cgctacctcg gccaggtgcg cctcgtcgca ttccacgatc agaacggaga
     8641 actcctcccc cccgaaccgg gcgaccgtgt cctcggaccg ggtggccgcc ttcatcaccc
     8701 gggacaggac ctgcaggacc tcgtcgccgg ccaggtggcc gtaccggtcg ttgaagcgct
     8761 tgaaatggtc cacgtcggcc aggatcatcc ccgcccggga tccgtgccgc gcggatcggg
     8821 ccaactcgtc ctcggcccgg cgcatgaaat agtcgtggtt gaagagcccc gtcttgggat
     8881 ccgtgatcga gctctcgtga tggagcccgt tctggatcgc gacggaaagg aaccgggaca
     8941 ggcggtccac gtacatccgc tcggggaggg tgtactccgc accggcgatc ttccggccca
     9001 ggaggatgat cccgtacagc ccgccgatgc cccgcatcgg gaggatcaat tccggttcgt
     9061 aggagaggaa atcccggtcg aaggccgaat cccccagggc ctgtcgggcc tcctcgaagg
     9121 tgcgggaccg ggaatcctcc aggaacagcc tcttcaaggc gcggtagtac cggatcggaa
     9181 cctcctcgtc cgcgggatgg agggaccggt agcagtactg gcggactcgc ccgccccggg
     9241 gaggctcgat caggaaggcc aggaagacgg ggatgaagcg gtccaggaac cgggagatga
     9301 cgaactccat catctccggt atccccgaca ggcccaggag tccggacacg tcgttcacca
     9361 ggctctcgag ctcccggttc tccctgcgca gggcttccag ggactcccag gtcccggact
     9421 ccgcgagggc cttgtacagg tcgagagcgc ccgcagtccc gcgggtatcc ttcggtccgg
     9481 agtgctcctc catgcgacaa gcgtactacg ggaggccccg gaacggctac gagggcacct
     9541 cgtcggcccc gtgcctcgcg ccccgttccc tttacaagcg gccccgaagc tgcgagactt
     9601 tccctatgga caaccgaacc gaatacgccg tctccgagat cggccggacc ctgatcggtc
     9661 gactcctgcc gggaaccgac ctggtgcccg ggatcctctc gatctgcgcg gaatccggga
     9721 tacgcttcgg gtacgtggcc tccctgatcg gcagcctggc catggtacgg ttcatctacg
     9781 tgaacaagac cccgggcaag gactcgggat tcgtgttctc caagcccgtg gagaaagcgg
     9841 gccccctgga gatcctctcc ggccagggca ccctgggaga aggcgaggac ggcaagccct
     9901 tcctgcacct ccacgccctg ctctgcgacg aaaaggaccg catctccggc ggccacatcc
     9961 tggagacggg ctgtccggtc ctggcgacca tcgaggtggt catccgggaa acccggggca
    10021 tacgcctcgt acggggtccc gacgaggaga ccgggatgag tctcttccgg gtatccagcg
    10081 ggttctagcg agccgccgcc cggggccggg acgggtcccg aggtgcgctc acaccgggaa
    10141 ctccacggtc acccgggtgc cgaccccggt ctcgaaccgg gccgagcccc ccagctgctc
    10201 gcttataatg cggaccaagg tcaggccgaa ccccggggaa accgccggat ccaggtcggg
    10261 aggcagcccg gtcccgtcat cccccaccga aaaaaccgcc cgtccttccc gcagttccag
    10321 ggaaatctcg atccggccgg tcttcctgcc ctggaaagcg tacttcatgg cgttggtcag
    10381 cagttcgttc aggatgatcc ccaggggaag gagcttcttg gcgtccaggg acaggggcgc
    10441 cagccgtttc tcgacggaga cctccgcggt cgaggggaac agccccgtca cggaatccgc
    10501 gatatccgcc aggtagcccg gcacggaaac agcccggacg tcctggccgg cggagagcat
    10561 cttggtgtac agggcctgca tgctgcgtac ccggcccagg gcctcgttca gggcggagtg
    10621 aacctccggg ctctccacgt acccggactg cagggacagg aggctggcga tggacgcgag
    10681 gttgttcttg atccggtgat gggtctcccg gaggagcagg tccttctcct ccaggccccg
    10741 gcggaggtcc tcttcggtcc tcttgatccg gtcgatgttc gtgtgggttc cgaacatgag
    10801 ggaaggccgg ccttccgggt cccgggtcat gacgcggccc tggtccagga tccagaccca
    10861 gcgtccctgc ttattgagca tccggaactc gcattcgtag ctgggcgccc tgcccgagat
    10921 gcactcgtcc agcgccgcct cggcatgaac gaggtcgtcg ggatggacca gccgcaccca
    10981 ggtggagtag tcgtaggggg tgagctccgc cagggtgtat cccagcatgg ccgcccaggt
    11041 ctcgttgaat actgtccggt tcgtccggac atcccattcc caagtgccca ggcccgcccc
    11101 gtcgatgatg aactggaggc gttcccgctc gtgccggagt tcctgttccg caagcccggc
    11161 cttctccatt ccttccaccc ccgacacctc ggactatcgt gccgagaatc gttcctgtca
    11221 agcgggatgc ccgtccattc gaggccaact atccgtgtct tccgccgggc ggggacttga
    11281 caaaaaatgt gaacgctgtt aacaatacgg acgtctcgag ttaacagcgt aaaccataag
    11341 tcaggaaagg acggccagag gtgcaggaaa cggacggccc ggacacccga acccggatac
    11401 tggacgcggc ggaggagatc gcccgagagc aaggcctgga ttccctctcc atgcgggccc
    11461 tggcggagcg gatcggcctg tccgccccgg cggcgtaccg gcatttccgg tccaagtccg
    11521 agatcgtccg ggccatgatc gatcggggac gcgacaggtt cgcgcgcggg ctcgcggcgg
    11581 cccgccgcgg gacggagggg ccggaggaga tcctggccgc ctcggcccga tactacctcc
    11641 ggttctggct ccgggatcgc gcgggattcc ggctggcctc cgagtggggc cggcaagaac
    11701 cctccctgac cggtcgcgcc gtggaggccg ggtccttcgg ggacgtcccg gccctggtcg
    11761 cggcgatcct gggggaaacc agggaccccg gtgagacggc tcgggtaagc cgctgggtcg
    11821 cgtcgaccct gtacggcatg gccctttccc tggtccagga cgaggggatc ccgagggccc
    11881 gggaaatgga actggtcgat tcggccgccg actatctggt cggggccgtg cgggccgccg
    11941 cttcggggcg gctttccggc gggggatccc ccgccggaaa taggggaact gcgcgccgga
    12001 acgggcgcgg aaggagcgat ccatgagatc ggagaaaaga cggcgggccg tggcggccgc
    12061 cgccgcgctc gccctgaccc tggggagttg cgcgttcatg ggggaaatcg accggatcga
    12121 aatcgtcgag ccggacctgg ccaaggcggc cgacgggacc tgggtcgggg aatggaacac
    12181 cgccctggtg tccgccacgg tttccgtgga caccctgtcc cacggaatct cgggcatcga
    12241 tattctccgg cacgagtgcg gcaagggcag gcccgcggag gcgatcgtgg accgcgtggt
    12301 cgccgcccag agactgaccg tggacaccgt cagcggggcc acggggagca gcaaggtgat
    12361 cctccgggcg attcaagatg ccctggagaa ggccgtggct gcgggggagg cgacgccgtg
    12421 aacatcgccg tccgcatcct gatcacggcc gtcgccgtcg cggcggccct cttcctggcc
    12481 gcgcggctcg ggggcctgga acggcgggcc cgtggggacg tggagggcgc acggtccctg
    12541 ccgcccgtct tcgactacgc ggcgggaccg gccgcgggga atccggggcc cgacgcggtc
    12601 ccggccggcg acggctgggt ggacatggcc acggccgccc ccggcacccc ggtgacggac
    12661 gccgatatcg acgccctgcc ggagccggct cggcgctact tccggcacgc gaacgtggcc
    12721 ggcaagcccc ggatctcctc gttctccgtg gtcctggagg gccggatccg gaacggcccc
    12781 gccgagtcct ggatgcccct ggtcatgcgg cagttcaatc gcctggacaa tcccgcccgg
    12841 gtggtctacc tgacctccac gaagcccccc atgaacggga tcgactcctt cctggacggg
    12901 cagggccgga tgctcatcaa ggcggccgac ctggtgaagg tcgtggactc ccggggaccc
    12961 gagatggcgg tgtccgcctt cgtcaccttc ctgaacgacc ttgtcctctg ccccctcgcg
    13021 tacttcagcc tgcccctccg gtggcggccg gccgggccgg acagcgtgga gctgtccatg
    13081 acccacgcgg ggatgaccgt ctccgcggtg atgaccgtgg acgccgaggg acgccccgtg
    13141 ggctggaggt ccgaggatcg ctacgcggag gtgaagggca ggaaccggaa ggatcggtgg
    13201 tccacgccct tcgaggggtc caaggagatc gcgggcctgc gcatccccga gaagggggcg
    13261 ggcatccacg actacgacgg gactccctac gtctatgtgg aactggagcg catccggtcc
    13321 ctggtcctgg acgccgaggg cctgccgggc cggccgtagc ccgggtcggg tccggagcag
    13381 cggcgccgtc cgggggacgc gccgccgcac ggctggaagg tccggcccgg ggagcgtact
    13441 ataggaccat gcgaaatcca tcggcgtaca cgcccggctc ctcccgacgg ccggcctccc
    13501 ggaccgcccg ggcgtgcctc ccggcgatcg ccttctcggc ggtcctcctg gccgcctgcg
    13561 gtccgggccg cgcggccccc ccgcgggatc ccgcagccct gcgggcccgg gccgcggagc
    13621 tgtcggcccg gctcctgatc gcggacgggc acatcgacta tcccgaccgc gccagcccct
    13681 acgaagacct gacggcgggg acctccggca acttcgacga gccccgggcc cggaagggcg
    13741 ggttgaacct cgccttcctg gccgcctacg tgccccccga ggacgaggac gccggtacgg
    13801 ccttctctcg ggcggaggag aggatcgacc tggtcctcaa ggccgccgac atccacccgg
    13861 acaagctcgc cctggcgcgg agccccgagg aggcccgggt ggccttcggg gcgggtcggc
    13921 tcgccctggc cgtgggaata gagaacgggg cccccctgga ggggcggctc gaccgggtgg
    13981 ggtacttccg ggaccggggg gtggcctaca tcggcctcgt ccattccgag gacaaccacc
    14041 tctgcgactc gtccatggag ccggatcccg agcaccggtg gaacggcctg tcatccttcg
    14101 gccgggaggt cgtccgggag atgaaccgcc tcggcctcat ggtcgacgtg tcccacgcca
    14161 cggacgacgc gatccgacag atcctggaac tgtccacggc cccggtcata gcctcccact
    14221 cgggactccg gtccctcacc cccggcttcg agcggaacct cccggacgac ctggtccggg
    14281 gcgtcgcgga atccgggggg gttgtctgcc tggccttcgg gtcctacttc cttcgggacg
    14341 cgttccgcac cacccacgcc gccgcggacc tggccaccgc gcgggacgtg gcggaggcga
    14401 tcgaccggac cgtccggatc gtgggggtcg accacgtcgg tctgggatcc gacttcgacg
    14461 gggtcggatt ctccctgccc aaggatctgg cggacgtgtc catgtacgtc aacctcgtac
    14521 gggaactcct gtcgatgggc tacggcgagg aggacctggc caagatcctg ggcggaaacc
    14581 tcctgcgggt ctggggcgag gtccgggcga aggcggagga atagagagga ggcgccgccc
    14641 gttccgcgct gcgcctcccg gaacgcggcg ggctcctaga agaactccct gaggctctcg
    14701 tcgattccct tcttcatctc gtaccagaac gagagccccg cctccttgcg gtccttgtcg
    14761 aattcgtcga tccaccgggc cagctcgggg tccgcgtccc cctccacgtc ccgtcggcgc
    14821 ttgaggaagc tcaccacgaa gtcgaggttc cgctcgctct cccagtagac cgaggcgttg
    14881 cggctgttga tccggctggc gcagacggcc gtggcggcca ggaagcggtc ccggttcccg
    14941 tagagggatt ccatgatctc cgggagcatc tcctcggacc agccccggtg gaatcggcag
    15001 acccccaggt tgtccatgac cagctccttc ttgaggcgct ccgcgcaggt gcggccgagc
    15061 ttgcgggggg gcaggaactc ggggccgtag tgcatgtagt acttgcccat gatggccatg
    15121 ggggccaggg ccccgggggt ccagtattgg ttggggacca tccagccccg ccgtccgttg
    15181 gcggtgtgga cgaaggagtt caggatgcgg acgcccctct ccttggccgc cttccgggcg
    15241 aacttgcgca ccccctcgga gaggtccacg atcccccgct tctccaggat cgtatcgatc
    15301 aggccctccg ccaggtccgc gttggccgcc gagtccgcca cggggtcgaa gcccgccgcg
    15361 tcgaacttcg gctcgcccga gacccccagg tccgccccgg caagctcgcc tcggtccagg
    15421 agatccatga accaggccag gaccccgccc agggagatac cgtcgaaacc cgcggcgtcg
    15481 gccttgtggt tcaggcgctc cgcggcccgc tggtcgaaga ccccgcagag ggggcccatg
    15541 gtctggtagg gctcgtagtc cttcttgaac tcgccgttca tcttcttgca gaccgcggcg
    15601 cagggttccc cgcaggtggc ctgcttcctg ggctggatgg tctcctcgtt gaactgcttg
    15661 aggtagtggt ccaggatgag gcgcttgtgc aggtccagcc ttgcgtcctc ggagtcgaaa
    15721 atcgtgcggt agttgaaggc catcacgcgg cctttcaacg tggcgtagtt cacgccgaag
    15781 gtcccgccgg tctcgaactt gggatcgaag cggtacttgg tggtgctctc gaagtccttg
    15841 gccgccaact tctgcttgta cttctgctcg aaccactcgt ccgccacctt gcggtcccgg
    15901 aagtcctcgt ccaggaaggt ccccccgaag accaccgcgg ccagcccgtg ttcctggagc
    15961 atgttcgagc cgaagccgcc ccggcccgcc caggtgtcca cgtaggtcag cttgcccttg
    16021 acgaccgggg ccgacccgac ggcgccgaag tccgtggacc gggcggcggg ccccgtcgcc
    16081 aggacccggg gatccgtctc gtagcggttc ccgtacttgt ccaggaccag ctccatgagg
    16141 ccgtacaggc ccccccggcc gtgggaccag gcctcggcca ggtccaccgg gatgacctcc
    16201 acgtcgatct cctcgccgtg gctccggttg agatagagga tggaggggac gggcgccttt
    16261 cccacgatgc tgatcaggtt gatcccgaga ttgtcgaagg ccaccccggc cccgcccatg
    16321 gaggagacgt agaagccccg ccagcagggg gagaaaccgt tgacgatcag gcggttggag
    16381 cccggcagga tggagcccgc gaagatcccc accccgatgt tgaggctccg ccagcggtcc
    16441 gtgatgtgaa gccccaggtc cacgggcccg aaataatccc cgaccttgta acgccggagg
    16501 cggtagaatc ccgtggacgg atcgacgaag agcaccctct ggacggcttc catgatcgtc
    16561 tccttggacc cgggctctgt ccccgcaggc gggtccaggg gtcagtattc tacccccggc
    16621 gcggggtgtc aatcggaggg accggttttc cggatgcgtc ggtcagccgt tccccagcac
    16681 cttggagtac ccctccccca gggccgagcg gaaccggtcc gcgggcaggg gcggggcgaa
    16741 gaggaagccc tggaagaggt cgcaggaggc ctcgccgagc cattcgagct ggcctgcgga
    16801 ttccactccc tcggcgacca cccggaagcc cagggcgtgg gccaggtcga tgacggcctt
    16861 gacgatggcc cgggaccgcc ggtccgaggg caggggttcc accagatcca gggggagttt
    16921 cacgaagtcc acggggaagt cccgaagccg ggagatcccg ctagccccgg tcccgaaatc
    16981 gtcgatggcc agggaaacgc ccagggactt cagctcccgc agccggggga ccgccgcctc
    17041 cgcggccagg gccaggccgg tctcggtgat ctccagctcc aagagggccg ggtcgagacc
    17101 ggtctcctcc agggccttcc acacctcttc ggcgatggcg ggctttcgga gggcctgggg
    17161 ggacaggttg accgccacgg acgcggggcc ggaagcgccc gcgttccact cccgggcccg
    17221 gcggcaggat ttccgcagga tgtcccggcc cagggcctcc atcagccccg cccgctcggc
    17281 cacggggatg aagtccgagg gcatgcccag gctgccgtcc ggccggggcc agcgggccag
    17341 ggcctccgcc ccgaccacgg ctccccggcc gtccaccttg ggctggaacc aggggaccag
    17401 agccccgtcc cggacggctc ccgccaggtc ccgctcgaac cggagccgct cgatcaggtc
    17461 cgcgtggagg gacgcgtcga aaacccggta ggctccggga ccgtcctcct cggcgtggtg
    17521 gtacgccgcg gtggccgccc gtacgagctc gtccggggta tctccgtcgt ggggatacag
    17581 ggctacgccg accactgccg ccacgtggat gaggtggctc cccgcctcca cggggcgctc
    17641 gaaggcggag aaggccttgg gcagaagctc ggcgatgtcg tccgggctcc cgagatcgga
    17701 acagagcacg aagaaaaggg cgtccccgaa gcgggccacc acgtcgtcct cccggaaggc
    17761 ttcccggagc cgggccgcgg ctcccgcaat ggcagcctca ccctggacat gcccgaactc
    17821 gtcgaagatc aggccgggcc gatggatgcc cacggccaac acggacaggg gcctgccgcg
    17881 gcgggacgcc gggaccaggg tcctctcgag ctcccgcagg aagcgctccc ggttcggcag
    17941 ccccgtgcgg ccgtcgaagt cccggtagaa ctcggccttg cgctccgccc ccagccgctc
    18001 cgcggcctcc cgctccaggg ccgcgctgcg ctccgccaac tggcgggaaa gcctctcccg
    18061 ctccgccagg gcccgctcca gggaccgggc cacgtaggac gcggccaggc ccgcagccaa
    18121 ggacatcacc accgcgttca agcccaggat gaccaggaag gcggagggcg cgctccaggt
    18181 cgggagcccc cgggacgcgg cgaaggtcac ggccaggaag gcggccccga ccaaggccgc
    18241 gcccaggact cctcccgccg tcccgaagag gacggcggcc aggaccacgg cggctcccat
    18301 ccagatcgtc cccgcggcgg ccggaccgtt cgcggccaga aggtagcacc cgaacccgaa
    18361 cacgagcccg accaagcccc ctgcggaaag gcggggcttc caccggttca ggagatacag
    18421 ggaaagaaag gcggcatagg cggacccgtc cgcgacgacc agggcggtct ttccgctccg
    18481 gatcgcgtac aggaggctgg ggaacaacag gaccgtcccg agtacgagcc ctgcccggat
    18541 gaggaacccc gagacttcca tgcggtagcg gtttccggcc gagtccaagg gcggaatctc
    18601 caggaaccgc tccggttcgg gaggcccggt ttcggatatc cctggtgatg caggaatcat
    18661 tctttgattg taaggtcccc gtaaacgggg cgcaaggcgg accggaaaaa ggtggattcc
    18721 cgtctcggga cagggggaaa aaagaaagcc ttccagggct caaccttgga aggcttggtt
    18781 catgggcggt acaagagtcg aacttgtgac ccccagcgtg tcatactggt gctctaacca
    18841 actgagctaa ccgccccggg gacacagagt tataccccga agcctcccgg cgtgtcaatg
    18901 tcgttcggaa ccgcggtacc gggtccgggg cgcggggatc gacgcgggca ccccgatgag
    18961 gcctctttgc gctttacttt cccacgacgg acaggaagcg ctccaggacc ttcttccggt
    19021 ccaggggctt cacgatgtag ttcttggccc ccgccaccag ggcctgcttg accaggtcct
    19081 gcttgcccaa ggcgctgatc atgatgacct tggcgttctt gtcgaactcc atgatcttct
    19141 ggagtgccga gagcccgtcc atcttgggca tggtgatgtc catggtgacc acgtccaggt
    19201 tcgggtagag ctccttgtac ttctcgtagc cttccaggcc gtccccggca gtggcgacga
    19261 cctggtagcc ctcggaggtg aggatctggt tcagctgctt ggccacgaac atggaatcgt
    19321 ccacgatcag cacccggtac ggggtgccct cgggcttgag cccgtccggc cgcctcgcgt
    19381 tgatctcggg aaaatcctct ttcgtcttca tgacgtgctc tccccgcgcc tagccgcgtt
    19441 cccggatggc gacgttgatc tcgagcttgc cctgggcggt ctccatgggg acgatgaggg
    19501 cctccacgtc ctggtcggag acctccatgt tctcccccgt gaagatcgcg gggggcgtca
    19561 ggtcgaattt gaagcccagc tcgtggagct tggtcaccgc ctgggcggtg atcatgttgg
    19621 ccagctcggt gatcgtggcc ttgacgagct cgtccatgac cttgagctcc tcaccgttca
    19681 tgaccgaggc caccttcaag gccgtgggct tgtccatgtc gaagagaacc cggccctcca
    19741 cgtcccccgc cagtcccacg atggcggcca cccccatcac ggggatggac gtggacttga
    19801 ggtagagctc gccccgcttg acctccccct ggaggacttc cttgaggacg ttgaacgcgg
    19861 attccacgaa gggattgatg tattcgactc tcatgtcact ctcccgtctt gcgtatcatg
    19921 ccttggcgaa cgcggacacc gccccggagc ccacgggtct ccaggcgccg gtggcggcgg
    19981 tttcgttggt acccaggatc aggactcccg cacccttgag tttctcctgg aaatcggcca
    20041 gcacccgtcc ctgatcctcc ggtgagagga aggacagcat gtcccggcag aggatcaggt
    20101 cgaccggcgg gaggggattg gcgttcagga tgtcgtggaa ctcgaagaag atggaatccc
    20161 ggacggcttg gtcgaaggtc cacccgttca tccccttgac caggtaggtc cggtacggct
    20221 ccggcgccga ggccgcgtcg aagaccatgt tgggggccat ggagatggcc aggaggtccg
    20281 agtcgttcgc ccagatcttg acccgggcct ggggatattt cctgcggagg gcgcaggcca
    20341 gggaccaggt ctcaaagccc ttgccgcagc cggggttcca cacgttgatg ctgcgtacgt
    20401 ccgtgtccgg gaggatcgag gcgaccgcgt ccaggtaatc ggcaccccag agggtgccgg
    20461 tgtagggcga caggaagccc tccaggaagg cgtccgcgtc cccgtcctcg tggagctgga
    20521 gatccgttcc cttccgcagg cgttcccatt ccgcgtaccg agcccggaac caggcgtcgt
    20581 tcacggcgga cacggcgaag tgacggaagg agaagagccc ttccttgacg aaaccggagg
    20641 tcatctcggc gtccacgatc gcagatcgtt cggtctgcga ccgttcggga gcggaccgtg
    20701 ccgaggccga acgggttccg gccgcggccg ccggtgcagc ctcgacccga ggcgcctcgt
    20761 agtgggcggg atgctcctcc ttctccgcct tgggggagaa gatcttggct atgtccagga
    20821 tgatgtagag ctttcccgcg ttctccacta tcccccggat gtacttgacg ttgatgtccc
    20881 cgaagatcgg gtgcgggggc tggatgctcg cccgggagat gcccaccacc ttgtcgatgc
    20941 tgtccacgat cacgccgaag acgtggtcct ccacccggag gatcagcagg ctctccacgg
    21001 cgtccgcttc ccgtttttcc acgggcaggt tgaacatgac ccggaggtcg atgacggaga
    21061 tgatgtcgcc ccggaggttg tagactcccc ggacgaaggg ggcggcgttc ggcacgtagg
    21121 tgaagcggcc cgcccgggcg atctccttca cgttcatgat gtcgatgccg tattccttgc
    21181 cccccaggga gaaggtgacc atcttgaagt ccaccttctc ggcccgctcc ttgcgcgtct
    21241 cctccgtccg ctcgaggatc atggtgtcca acggattcgt ctggctgctc atgctggcgt
    21301 acccccggcc ctcatcggat ggctccctcg agacgcgctc gctcttcctt ctcccgcttg
    21361 agccccagct ccagaagctg gctgacgtcg atgatgaggc acaccgagcc gtcccctagg
    21421 atggaggcgc cggctatgcc cggggaggcg gtgtactggt cccgcagggg cttgatgacc
    21481 acgtcctcct ccccgatcag ggagtccacc atgagcccca cccgcttctc gttggtgccc
    21541 acgatgacca cgaagttgta ctcccgctgg ccggaggtgg ggatgcggaa gaggcggtcc
    21601 aggcgcagga gggagatcac gtcgctgcgg acgttgaaga cctcgtagtt gtcgatcatc
    21661 cggatctcct ccggcttgat ccggtgggat tcgatgaccg aggtgatggg gatggagtag
    21721 gtttcccggc ccaccctcac caggaggccc tggatgatgg ccagggtcag cggaagcttg
    21781 atggtgaact tggtgccctt gccgggcctg gaggtgacgg ccacggagcc gttcagtttc
    21841 tcgatctgcc ggcgcaccac gtccaggccc accccccggc cggagatgtt cgtgatcgcc
    21901 gcggcggtgc tgaatcccgc ctcgaagatg aggttgaagg cctccacgtc cgtgaggacc
    21961 ttgttggggt gtatgaggcc ccggtccacg gccttgcgct tgaccgcctc cacgtcgatg
    22021 cccttgccgt cgtcctcgat ctcgatgatg atcatgttgc cttcgttgga ggcccgcagg
    22081 tgcacggtcc ccgtctcggg cttgcccgcg gcgcggcgga tgtcggggct ctccaccccg
    22141 tggtccaggg cgttccgtac ggagtgcatc aggggatcca ggaggtcctc gatcacggac
    22201 ttgtccagct cggtgtcctc gccctcgatg accaggtcca cctgcttgga aaggctcttg
    22261 gaaaggtccc ggacgaggcg ggggaagcgg ctgaagatct ggctgatggg caccatgcgg
    22321 atccgcatga ccccctcctg gagctccccg gtgatgcggc ccaggttctg ggcggtggac
    22381 cggaacttgg ccaccgtgga cttgaactcc gcctcgaagg gatcgtgggc cgccgccagg
    22441 acggcccagc gttcctggag ggccttcttc atgtccttga cggacttacc ggaaaccagg
    22501 gcctcgttca cctcgtcgaa gagttccttc aggcggtccc ggaattccga ctgcaccgcc
    22561 aggaaggtgc cctggagctc cgagaactgc gagctgatct ggttgaaggt ggccttgttg
    22621 atgaccgtct cggacaccag gttgaggagg ttgtcgatgc ggcgggagtc cacccggagg
    22681 atggagccct gctggacctt gggcgcggcc ctccggtccc cctcctcggc ggcctcctcc
    22741 ggatgctcct cggcgcccgc ggccggggcc gaaggctccg gaaccgcgcc cttcgcgggc
    22801 gcggaggccg gggcggccgc ggaggccgcc ggagccgcgg ccgcctcctt cttccgggcc
    22861 accgcccggg ccagctcgtc ctcggtgacc gcccccgggg acggagcctc cgcccggggg
    22921 acagaggact tggcaggggc gggcttggtc gccgcgggag ccggagccgg ccggccgccc
    22981 tcctcgaagg tcagttccgt gacccggacg tccttcgtga cctcggggat gcgggccttc
    23041 cggtccaggt cctccgcggt cctgtccgtg gccaggtaat agtccacctc cgggaagaac
    23101 tggtcctcgt agagcttctc gaaatcggga tgggtcttga gcaccgtccc cgcgtcccgc
    23161 agggccgcga aggcgtggat ggcgctgatg gtcttcatca ggttcgactc gtcgaagacg
    23221 acggtcacgc ggtacacccg gcggtccttg cccgccgcct ccttgagctc cagaatctcg
    23281 tactccgtaa gacccgcggc ggacaccgat tgggcgggca cgtcccgggc ctcgggctcg
    23341 ggagccggcg cgggcttggc ctttccgggt ttggacgcgg cggccttggc cccgcccttg
    23401 gccttggatc cctcgccggg aaggaagccg gacagggctt cccggacggg ggacacgtcc
    23461 ccgtcgaaga ccgaaccgtc catccgggct tccagcatgg ccttgaccac gtcgatggcc
    23521 ttgagcagga cgtccacggc ctcctcggta accttgagac ggtcggagcg gatctcgtcc
    23581 agcacgtcct ccacgaggtg ggtgaagccc tggagctcca tcatctgcac cgtcgcggcc
    23641 ccgcccttga gggtgtgggc cgcgcggaag atttcgtcca cggcgtcccg gttcccagga
    23701 tcgttctcca gcaccaggat gttctgttcc agggcgtcca cctggctctg ggcttcggcg
    23761 aaaaaatcct tgaggagttc ctcgttgttc gggtcaaggt aatcgctcat tatggtatca
    23821 tcttatacgg aatggagatt aagtcaagat tcgccggaag atcgcgacat ccggcgaaaa
    23881 tgatatagaa tataggcgga gagtttccga aacttaacct agccgtgaag ccttgtacga
    23941 ggtgacccga tgagaacatc ccgcgcagcg gccgccgccg cgctgatcct ggcccttgcg
    24001 gccgcacccc tgtccggcct ggagatcgcg acggaagccc gggcggggaa catcttcttc
    24061 ccctggcccc aggccgccgc cacgacgggg gccttcccct cggacaacta cttctggggc
    24121 gccgccctgt ccgtctccga ctcggtcggg gacagccttt cctaccggat cggatacgag
    24181 accgatcccg tcctccggca cctggtcacc gtcctcttga cctacgacct gggcttcgcc
    24241 cagttcagcg cgggcccctt cctgggggcc ttcaactccc gggaagcccc cctgaagggc
    24301 gggctctcca cctccctgcg cttcgaatgg cccggccggg tcttcgcctc catccggtcc
    24361 gactcctccc tgggcggggg ccttttggcc gcgggggact acatccagga gaccacggag
    24421 atcagcgcgg gatggtacgt gtacaacgcc atctgcaccc tgtccatgct gaccaagaaa
    24481 ttctacatcc aggaaaccga caccctcatg acccaggacc gcctggcccg gtacaccttc
    24541 gacgtgaacg tataccggaa gggggctccc ctgaccctgg tctggaccct gggctaccag
    24601 gccctgtcca agacctggga cgacggcacg cccgagacag acactttggg gagcgtgatc
    24661 ctggggaccc gggcggactg gcgggtggcc cggaaactgc gcgtcacggc gcggctggag
    24721 accagcgtgt tctccttcgg ggccgacggg ctgcnnnnnn nnnncaggga cagagcccgg
    24781 acccgggggc cttcctcttc gacgcgggcc tgggggtgat ctaccggatc ggggagggca
    24841 cctcgggcgg ctctccctcc gcgccgtaga ccccggtcga cctactcgtt cttcagcgcc
    24901 cagcgcccct cggcctggga gaaccgtgag gcgtccacca ccaggacccg gccgtcctcc
    24961 ccggagatgc gcaccttgcc ggcggggacg ttgatctcgg tgacccggaa gaccaccccg
    25021 tcccacggga accgcgcccc ctcggggggg atgctccgcc gggcttcccg gtagaagccg
    25081 aactcgtagg ccaggcagca cagcaggcgg ccgcaggggc cggagatctt catggagttg
    25141 agggagaggt tctggtcctt ggccatcttg atggacacag gcaccagctt gtccgttacc
    25201 ccgtggcagc agaggacccg gccgcagacc ccgcagcccc ccgtgacccg ggcctcgtcc
    25261 cggacgccga tctgccttag ctcgatccgc atcttgaaga ccgataccag gtccttgacc
    25321 agctcccgga agtccacccg ggaatcggcg gtgaagaaaa agaggacctt gggctcctcc
    25381 agcaggtagt gggaggacac gagcttcatg ggaaggcgcc gggcggctat ccggtcctgg
    25441 cagaccgtga aggcgtgccg ttccttttcc gcgttctggg ccttgcgctc caggtccgag
    25501 ggcgtggccg gccgctcgat ccgggcgatc tcgtccgggg acgtgtaccc ggggttccgg
    25561 atgagcccca tgacccggca gagatcccgc ccgtaccggg tggataccag gacgtaggag
    25621 cccacggcca ggatccgggg cgccggagcg ggggcttcgg gctccgcgga ttccgccggc
    25681 gttcccgaaa cctgggcggc cggagactcg ggaggggatt caggcgcgtc ccggccctcc
    25741 gcgctcccgg cggcctccgc caccgggggc gccgtcaggt ccgggacctc cgggaccggg
    25801 accgccgggg acagctccgg gggctggggg acgggaccgt cgtagacggc caggaaggtc
    25861 tcgtgggagt gctcgatggc cagcctgtat aggggccagg gcagggactg ggacggccgg
    25921 gagtccttcc gctcgacatc cgcaccctcg tcatccaggt gccggagttc ctcctcggtc
    25981 atatcgtgat ccgcgtagcg cgtcatcgtt cctccatgag attcactgca ggagatccac
    26041 caggatcccg tagatctcgt ccaccttgcg caggatctcc agctggttcc gctgggtgga
    26101 caggatctcc gcggccagcc tctccagttt ctgatccacc acggacagga taacgtattt
    26161 cttgcgtttc aggatattgg tccccgaggt cttctcctcc agggtatacg cccgggatac
    26221 gacgaagtgg acgaaattgc gcaccgccag cttgtaggcc acaaccgcgt ccgggttggg
    26281 gtcccgcttc aggtcctccc ccaggccgtg gacgtcgtcc agcagggcct ccaggtccgc
    26341 ctgggaaaag ggggcttccg ccacggactc ggcgtgaaac ccgtcctccg tcctctcggc
    26401 gtccctgagg agggtccgga agaccccctt gcccttgtcg accttccgtt cctcccgcgg
    26461 cttcctcccg gagagtccgg gcacgagccc cgacaggagg ttgtccggta ggtctatttt
    26521 cgccatgggc cttccgtccc cgcgcccctg cggggatcga cggggtccgc ggccgggacc
    26581 cgggtcccgt gccgggcctg gaagtccagc agggcgtcct cggccttctc gaccttgccg
    26641 ctgacctcca cgtccccgtg gatgacgatg tccccctccg cgtccaaccg gggggcgaag
    26701 acgttcccga cgatgaccgc cccgtccagg accgaaagcc gttcggtgac gcagacgtcc
    26761 cccttgacca ggcccccgat gatggcggac cgggcctgga tcgaggactc gcagcgcccg
    26821 ccctggccga tcacgacctt cccggtggac cggatggccc ccgtcacgtc cccgtccacg
    26881 cgcagaagcc cgtccacgac gaggtccccc cgcagggaac tgcccgtccc gacgagggaa
    26941 ttcaccaggg agtcacggag gttcgccatg ggggtatcct aaccggggga ggctactgcg
    27001 tcaacgcgga gctggagcgg atgttcagga accgcagggg gtcgatggtc tcggtgccca
//
LOCUS       DAOTEI010000029        26303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1209_length_26303_cov_5.501676, whole genome shotgun sequence.
ACCESSION   DAOTEI010000029 DAOTEI010000000
VERSION     DAOTEI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 26303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26303
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_26303_cov_5.501676"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..763)
                     /locus_tag="P5313_04515"
     CDS             complement(140..763)
                     /locus_tag="P5313_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRY79659.1"
                     /translation="MKLDWKKTDKAFYLPGTEPELVTVPAFKFFAIRGAGDPNEPAFQ
                     DYMAVLYALAYAVRMSPKSGTAPAGYLEYSVFPLEGVWDVSEEAKARGGAGISKSEYV
                     FDLMIRQPDFVTPEYALSVIDRTKARKPLPRLGDARFEVLEEGPCVQMTHRGPYDAEP
                     ASFARMEEFCAARGLRRLSKVHREIYMSDPRKTAPEKLRTVLRFRVK"
     gene            complement(837..1820)
                     /locus_tag="P5313_04520"
     CDS             complement(837..1820)
                     /locus_tag="P5313_04520"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRY79660.1"
                     /translation="MTRRELLRREYLGRVERAIDFIYARFGDDIGLDEIADAAAFSRF
                     HFHRVFSGVVGETVSDFLKRVRLQRAAALLADNPGLSVTEIALASGFSSPSVFARAFR
                     ERFGMTASEWRDLGREGRRFLRDGSKQGQALGMPGQGDRKDRKESADAGGYPPPIVPD
                     GTRRKKMPKLSYKVEIKDLPELTVAYARHVGSFDGIPEAFGRLARWAGPRGLFALPGA
                     RTLAVYHDSPEATETAKLRSSACLTVPPGTEVSGDINLMTIPGGKFAVARFEIAPDQF
                     GAAWDALMGEWFPSSGWQPDDRMCYEVYLSEPDAHPEGRFVIDICEPVRPL"
     gene            complement(1822..2673)
                     /locus_tag="P5313_04525"
     CDS             complement(1822..2673)
                     /locus_tag="P5313_04525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY79661.1"
                     /translation="MQEFMTLEAESLQVVYAVFMEAFSDYSVPVTWSFEEFRASCLRR
                     GVDLSISLGAREDGRLVGFVLNGRGTWGGMPAAYDAGTGVVPAARGSGLAGRMASRAL
                     DLLAARGIRRYVLEVIRDNLPAFRTYVKAGFEVTRSLECPGGVFTDPGKLAPVGLTVE
                     ESGREGLDGKAAAVRRDWEPSWQNSDDSVRRAVEPLVVLEARRDGRSVGHLVASSTGT
                     VWQLAVDRDYRRRGVGTALLRALASKLGPSIRYVNVQAGDGATLGLLSACGIGPGVGQ
                     YEMIREL"
     gene            2877..3389
                     /locus_tag="P5313_04530"
     CDS             2877..3389
                     /locus_tag="P5313_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY79662.1"
                     /translation="MPNDGSTGADPGIVIIPVRDGPGLEAARTLIREYLAFLDADLSF
                     QDIDAELRNLPGKYAPPSGTLLLASVHRDPGPGKPAGCGALRDLGGGYCEMKRLFVRP
                     EYRGYGIGRALAERLLEEGRALGYRAMRLDTLDRLGEAVALYRALGFRPIPPYCENPL
                     PGAMFWERDL"
     gene            3469..5253
                     /locus_tag="P5313_04535"
     CDS             3469..5253
                     /locus_tag="P5313_04535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79663.1"
                     /translation="MKRFGATLLVILLAVPRILPQAQALDPVRLQLKWHHQFQFAGYY
                     AAKEKGFYAEAGLDVTLLEGDPDVSYTDEVVSGAADYGVNNTDLLIDRSKGKPVVVLA
                     AVFQHSPTVLLTLKGRGFEVPSDFAGRSVMIVPGAEAEVTSMFRYESIPLEAVRFRPH
                     SWDLDDLLSGRVDAVTAYITNEPIILKASGIDYTLIRPLTYGIDFYGDCLFTTESEVR
                     RRPGRVKAFLEASVRGWDYAMHNPEEICDLILAKYSTRKPRDVLLQEAEAMRELILPD
                     LVPIGFMNPGRWKHIGDTFASLGSLPADYSLEGFLFDPRAPRLDPRVIQAGLVFAGLA
                     LFAALAYILALRSFNRLLAFKVETRTAILRETNRLLEQEILRGAEREKRLAASLSEKE
                     VLLREIHHRVKNNLQIIVSILNLQAEKGDEVFLRDLRSRVVGMALVHEHLYSGADLGR
                     VDMGQYMGDLVSDIAASYSRPDLSIRTDVRVADVSLSIDQAVSLGLIVAELVSNSMKH
                     AFTGRRSGSIELALGREDGSYVLSIGDDGPIAETGPQSASAGSAAREGLGLRLVEALA
                     AQLRGDLSVDRTRGYRTSLRFPAALNGT"
     gene            5450..5842
                     /locus_tag="P5313_04540"
     CDS             5450..5842
                     /locus_tag="P5313_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY79664.1"
                     /translation="MDRHLAPSGFTTRQWFLCLVLEQAGRSLSLRETALRMGTSHQNA
                     KQLALKLERKGYLRIERDPEDARALRLSLTEACRAFWARRKDRDREILEAVFRGLPAP
                     DLDALHRALKRLDLDVHTLLADDSLPFL"
     gene            5852..6718
                     /locus_tag="P5313_04545"
     CDS             5852..6718
                     /locus_tag="P5313_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79665.1"
                     /translation="MKPVTNTQKALALGIAVLSLFACLAGLAPGTPRPDSPAAFTSIR
                     GERVELQGTGLYRLDSVSFAAQAKAQDLVTLLLGIPLLGISLVLAVRGSLRAGVLLAG
                     TFGYFTYTYATYAFGLQYNPLFLVYIALFGSSVFGLILSLAGLDTVRIREGFRGPRVR
                     KAAVGFDFLAGTLIFLMWMARLLPNILAGTDTTFIEHYTTLPIQVMDLGFVVPLALVS
                     GVNLARDRPLGYLLTGLFLMKGVTLGLAVGAMILWSVVAGLPVSPAETAVFAVIIGAG
                     VGLGAAWLGAIR"
     gene            6792..7793
                     /locus_tag="P5313_04550"
     CDS             6792..7793
                     /locus_tag="P5313_04550"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY79666.1"
                     /translation="MRNRLHRGRIAAAGLAASLLGAFGLAACVAPAPRGGPSTAEEAV
                     ATADGAWIAARDGVLLRVHAWEPAGAARGILLLVPGVTGLDTRMEAPVTEPLAAAGFA
                     VFALEPRGTGESGGPRGYAGNASLILDDLGSFAAWGRERAGQGGTAEGLPVFLFGHSM
                     GGTFALALAAEMETPPAGLVLVNPAYKYRPLKGATPGFGDYLRFAVHWIFAPRKPVVD
                     MGGDPSLLEDPDDRAEAVERKASPAVQGFQSMASMTAARKIMTAAPRNAARLSAPGLI
                     VEGKRDLLIDPAGTDEISAAWGGRGAERIRVDGAHGRSSALAGMETILRWLAEEAGR"
     gene            7790..8638
                     /locus_tag="P5313_04555"
     CDS             7790..8638
                     /locus_tag="P5313_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY79667.1"
                     /translation="MRGTELDGKVAVVTGGGSGIGRALCGELARRGARVVDTAGAEET
                     AAGIRAGAAGPEAGPRALARRVDVTDPGDVEALMAETVRDFGRIDFLFNNAGVSANGE
                     FQDIGPEHWKRILDVNLWGVVHGCRAAYPRMVRQGFGHIVNTASLAGLIPGSLTSAYS
                     ASKHAVVGLSLTLRAEARTRGVRVTALCPGYIRTPILKTTPTLSPYMDSPRNREMNEG
                     LRFPGPEGCAPAMVRGVLRNKAVVIAPASQAIYWLLYRLAPALLPWAGSKIVETMRRN
                     AEADGR"
     gene            complement(8741..9484)
                     /locus_tag="P5313_04560"
     CDS             complement(8741..9484)
                     /locus_tag="P5313_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79668.1"
                     /translation="MRNKGLWMVLALIAVSAAAFAQAPDPLAAGGWEFGGTVKFSHNP
                     DYPIFAGDVTEEEKGEYLQITEASISVGRFLADRFSLALRPSMLLYSRHTLNSSLQES
                     ISRDLFLGLGFEPSWYFPLGSSWILGVGAELGVGIWPGLPGVSDDVRESDHSLSFQLW
                     LEPKVRAYYRVSELLAPYAEAGFRLGVIRRVRDAFGDPYDPGVPLLDEVRGRFAFTLG
                     LKYFLPQGARFVETQKTPVNDWYDMSGGR"
     gene            complement(9634..11376)
                     /locus_tag="P5313_04565"
     CDS             complement(9634..11376)
                     /locus_tag="P5313_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRY79669.1"
                     /translation="MRFRNFAVLAFLAAAGMLAGCAAAGAGGEDPTLEQVAAPTFTPS
                     PGTFSEDQSVEISSATGGAEIRYTTDGSAPGGSSTLYTVPIAIAGNGTTMTIRAFARM
                     SGMSDSDAADATYTIDYSQVSTPQFSPAPGAVDPGTVVTFSCATSGVTYHCTTDGTDP
                     TTGSPSVSSYQVDGPVTLKLLAVKAGMADSPVAVATYAPRLPAPGLSPAAGTVEGGST
                     VSFSSPVAGVTYHYTTNGTTPTTGSPSATGIQVNDPVTLKVIAVKAGWTSSGVATAAY
                     TPKVAAPTLAPGSGIVSHAAAVTFGCATSGVTYRYTVDGSDPTGTSASGGSYSVDMSP
                     VTVKVMATRPGWANSDIASQTYTVNPPAAPTGFSVNLAARGWDSAGWRYDIDPAAWSH
                     SGINLGWFDIEVFVDGAWRYVVGATGIPGSRQANISFAQYYPSGKTGTVVWIPESLGE
                     YAFRITARNAGGTSAPATTTARTEIGTPALQLYSYTMAAPWQSTVRIYQLSDGGVHGY
                     KAERYNPDTGLWSEVGSALIGAKSWNVSQWVLDHTDTRPDNTKAYKYRVRGYRDIGGG
                     VLQYSAYSNEVSCR"
     gene            complement(11457..13004)
                     /locus_tag="P5313_04570"
     CDS             complement(11457..13004)
                     /locus_tag="P5313_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY79670.1"
                     /translation="MASAINELCNRTNGSSAETRRPFLPSAFLTCSLLFGWTLAYFYQ
                     SSPWIILTGPDGGADSARLFYFGGFLIGMFAGLSFMRRFNVKAMVSGCAAAALAAILV
                     LRFIPPARAGGCGFGLFGILAGTSFGAMFHFWVYAFPGLPRVSSIAAVHLGGGAVSLA
                     VLWAGERFGTPGAAAMDLAVPLVALWAALRVDMSRVEDPGSGPPLTFPGRLMGIVIVL
                     LLLVYFATFLPEGLLSVTGGSAWTTPSPGVRVLVSSAVYLLFLRKGSGIHILLLLYSM
                     ILCQLIGYGLIILHAGTSAGSLVLVVGEALGNLFLFKLTFDIFHKHRGRPLIVILFTG
                     AVVLGTSVGMLIGWVLELSGRRDTASFVGMMVVFYSTALALLPPLLRVLEEELSLRSL
                     TRIGPPGSRADAPSSPEYEEEGHVAILDPAYIREPMGRKIRRINERFDPPYRLTEREI
                     EIAALLAGRLDYGSIARRLDISVNTLKTHAKSVYRKFDVPGRKGLIELCLEPDRRSAA
                     EERAAEA"
     gene            complement(13042..13644)
                     /locus_tag="P5313_04575"
     CDS             complement(13042..13644)
                     /locus_tag="P5313_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79671.1"
                     /translation="MGRSKRVADPGDIVQTAYDLIDREGYEDFSARRLAQILGVSHMT
                     LYNYLAFGEILDGVIARGFEEITEALKPRIPEEVEGPCRPCALFKIIAEELLEYAKRR
                     PNLYRFVFQSGFGPTTGDPKVRRLYWSGIDLIPGLLDPVRQEELRRDGFLFLVLANGL
                     ILAFLSGRHDMDEGSCRRNIERGYSLILGGYSGRVSSPSR"
     gene            complement(13721..13858)
                     /locus_tag="P5313_04580"
     CDS             complement(13721..13858)
                     /locus_tag="P5313_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79672.1"
                     /translation="MARRGGEGKAVVFGLPGDRFGYHAPLVFGMEAGRNAVLSTGYSV
                     S"
     gene            13880..14278
                     /locus_tag="P5313_04585"
     CDS             13880..14278
                     /locus_tag="P5313_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79673.1"
                     /translation="MFHPKTTDWDNRMKEMFDRIDSILEDRYHGSWRLRRNRPGRGET
                     SNPEADGLFNVGVFFTPGFGSRLGRGYLVEIVLATEEAVPRAKRDEIELSVRDLLLEF
                     LPVYFPDRRLTVERDGSMYKIRGDLGLGGV"
     gene            14336..15391
                     /locus_tag="P5313_04590"
     CDS             14336..15391
                     /locus_tag="P5313_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 43"
                     /protein_id="HRY79674.1"
                     /translation="MTISDFLSMRWSGIEGPLIEPPRLSPIVADPSFLFPEETPGGVW
                     ELFAHSAWGIHRYTSPDGARWTERGIAVHNAMRPFVRRLDGEGLCPDEGPATGSGRRG
                     AAPTAAGPAGKPASSRFGLYYESYPPLALPLTALPFRRRWRSRIAVAVSSDLERWSPG
                     RTLVSPLLPWMRDRSLGFAVSNPCLVRDWTDTYRLYFSASLAWIEDCGFCEPKYVGLA
                     RGASPEGPFVPDPEPAVDPTRDPMPGVQGAGAIKVLPLSDGYVGLQNKIYRDPSGRSR
                     SALFVLRSEDGVSWEPARREPLLAPGSGWTASHVYACDCRFRESDGRWYLYFNARDGW
                     RISEGRERIGRIVGTPG"
     gene            15495..16235
                     /locus_tag="P5313_04595"
     CDS             15495..16235
                     /locus_tag="P5313_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY79675.1"
                     /translation="MPDPTPPAVLVADDDDNLRASVRASLEREGFGVREARDGVEARD
                     FLAGAPDPAGRPALVILDISMPRLSGLDLLRELRTRGDTIPVIFLTSRDEELDRVLGL
                     ELGADDYLPKPFSLRELAARVRAVLRRVESRSAPAAADPPAAGTRESPAPGSLTLDRD
                     GFRAFWAGTELDLTVTEFRILDCLAERPGAARTRDSIVARAWPGGVYLSDRVVDCHVK
                     RLRRKLREAGAGDEAIETVYGLGYRLRP"
     gene            16232..17884
                     /locus_tag="P5313_04600"
     CDS             16232..17884
                     /locus_tag="P5313_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY79676.1"
                     /translation="MRRPRVPIVARILGVNALLVFLPVAAFLSLDTYERSLLSALEDS
                     LVQQGRFLAAWLGEGELSASRAEAVLAALGRRHTARLRVLDAAGNLLADSSVRPPDAP
                     PSAPDAEAPYRAGPDVLPEAKPAESSWIYRLLSGPVRLWRRYLAPPSEPLASADFYTG
                     KRNFLEGAEVRAALEGRYGAATRISAGGQVSVTLYSALPVPSPDGTPRGAVLASQSTY
                     RILAGLYRLRLDVGRIFLAGLAASSALSLLLWLTISRPLRRLGIQARAALSLRGLAEG
                     HFKPGAVSDEIGDLGSALREFSARLGERLSWAEDFASDLSHEIRNPLASIRAAAELLP
                     DAGPEEVRILGDRIARDADRAARIVGGLRELSRIEGGEDGTGPADVLAVARNCARFAA
                     DRSGSGSGPTDTRITVEAAPDAGELRAAVSPDRLALALGALLDNAVSFSPPGGEVEVR
                     ADRTREADGEKIRLSVLDRGPGIPPEHRDRIFDRFFTFRPAGRDGNVPGTRAAEQQAS
                     EHQGLGLALVRGIALRAGGRVETADRPGGGTVFTLVLPAAPR"
     gene            17949..19352
                     /gene="creD"
                     /locus_tag="P5313_04605"
     CDS             17949..19352
                     /gene="creD"
                     /locus_tag="P5313_04605"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HRY79677.1"
                     /translation="MNRTPRLPRAAQTSLKAGLVGVLVLAFLIPVGMIREVRSERESY
                     RENALEDVSSKAGGRTLLITPYLAVPARYDVRETNAKGEEVVRTRKAVYIVFPRTLGL
                     TCDTRVELRKRGIYSVPVHRTEAAMDLEFDLRSWETGIPGVRVGWTEAQLYYAYDDPR
                     SLRGSPSLAATDGSRAVLRSSPAPLELAARSVSVPVRLQPGTDGSARYAARLELSLNG
                     AEALDFLALADTNTLRLSCDWASPSFSGYRLPADRELGDAGFRAEWFVDEPSRVLPRV
                     LAASDFRPESVAGAAFGVDFLQPTDVYQQVHRALRYAVLFLFIPFAALFLFEVLARRR
                     LHVVQYVLVGLANCLFYLLLLALAEHLPFLAAYLVSAAAVCGVTTFYVGAFLPDKRQA
                     ALAFAVLALQYGYLFCALSSEDYALLIGSVGLFGITALTMAATRKVDWFGGKGEAVPE
                     PDGEPQDLPEFPPLREP"
     gene            19426..19893
                     /locus_tag="P5313_04610"
     CDS             19426..19893
                     /locus_tag="P5313_04610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HRY79678.1"
                     /translation="MAETMVLREVGAVERRDGRTWVRIRPEYREALRGLEEFSHCHVL
                     WWSGSDFGMGFDTRSVLSGELPYSPGRTAGVFGCRGPFRPNLIAMTVCPIRAVDPEAG
                     RIEVAAIDAFDGTAVLDVKPYYPSVDRVRDARVPAWLPDWGEWAPDGGFGLDE"
     gene            19919..20752
                     /locus_tag="P5313_04615"
     CDS             19919..20752
                     /locus_tag="P5313_04615"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRY79679.1"
                     /translation="MDNAQVILETARWIEGRLREDLPVPAIAAKAGYSLHHFTRLFAA
                     VIGMPPKEYVLRRKLSEAARDLAGGRRVTDTAFDWGFRDLDSFSRAFRRELGSSPSAV
                     RRGAPFPYQEPAVRRIPGPPVDAEPVLERFPPVLLAGWQVRITGPGDEVGRLWAGFLP
                     RAASINRRIRPPRVRQLATWTEDAEDWVDILVAAEMETLDALPLDLVGRAVPGCECLS
                     FLHRGSAARVGESYAAIYGTLLPSLDRKPSLPFNFEWYFEDAGDPYSEDFRFKICVPV
                     A"
     gene            20820..22019
                     /locus_tag="P5313_04620"
     CDS             20820..22019
                     /locus_tag="P5313_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79680.1"
                     /translation="MGFFKKIRTTAVKAVRALTRRASTVLGSLRKSSSPLADRLLSDA
                     LRIAEIPSPTAREEQRAAFVLERLRSLGLNPQVDEDGNVLARITCPVPDDPRPILVLA
                     ELSSPRWHPLESLSRLDADRAYGAGLSDSLGPATLLSLAESLQSGTPAIPKDVLLLFA
                     ARPLDDPRGSVLRRLAEDPQDRPAASLSLRGLSLGTVPTKSLGTYRIEVRVRTDAEVS
                     ETDRGPRTAVDAVVALARTLTGITWDAEKRTTCAIRRIEAGSGFGHEPTEGLLDIELE
                     SADEAVLEIAMKAATATAESRGREANLQTQVTIAGYVPVGDPGAGAGLSKLVIQALKE
                     QHIKVREESGADPAAFFSSRGIPAVSLAVASGREGLDRDEIEVASIEKGRRLLVSVIE
                     RVSKEVL"
     gene            22016..23029
                     /locus_tag="P5313_04625"
     CDS             22016..23029
                     /locus_tag="P5313_04625"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HRY79681.1"
                     /translation="MSKPSDDSLLVRTWHHSKFSDIGRLVDLKEKKGLTLSLAFPTLN
                     EEATIGKEILVIRTELMDRYPLLDEIAVIDSSSKDRTRQVAERYGARVFESKDILPGR
                     GTFRGKGENLWKSLYALQGDIIVWVDADIANIAPKFVYGLVGPLLEDDSIGYVKAFYE
                     RPIRSSSGIQSSGGGRVTEILVRPIFSLFYPELARLVQPLSGEYAGRRELLETLPFSV
                     GYGVELGHLIDVYRKFGIGALAQVDLDLRIHRNQSTEALGRMAYGILATFLDRARRYG
                     DAEVLRELGDRHIALKVEKESHTVAATEISAVERPPMVEVPEYTQKFKESGRHWAGRN
                     LEA"
     gene            23108..23449
                     /locus_tag="P5313_04630"
     CDS             23108..23449
                     /locus_tag="P5313_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY79682.1"
                     /translation="MSEDTILESSPTGKRLQSLRKDDTLSILLCGKRIPLIGKIVLGR
                     DRSCGIVLEHALVSKRHAMIQKIKDDYFITDLSSTNGTFVNGKAVPPGKYVRVEPGDK
                     IKIGKTVLTIK"
     gene            complement(23541..24839)
                     /gene="eno"
                     /locus_tag="P5313_04635"
     CDS             complement(23541..24839)
                     /gene="eno"
                     /locus_tag="P5313_04635"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRY79683.1"
                     /translation="MSVIEYVEAREILDSRGNPTVEVDVVLEDGTVGRAAVPSGASTG
                     EHEAVELRDGDKKRYLGKGVLKAVENVNNVISGEIIGLDATEQVDIDRTMIDLDGTEN
                     KGKLGANAILGVSMACARAAANYLETPLYKYLGGAHTTLLPVPMANIINGGKHADNKV
                     DFQEFMVMPVGAQSFREAVRMTAEVFHSLKGLLKADGHNTSVGDEGGFAPNIGNEESL
                     DYIMKAIEKAGYKPGDDIAIALDCASSELFDEGEKKGYKFWKSNPGKLFSADEMIEIY
                     TKWVGKYPIVSIEDPLDQNDWDGYVKMTKALGGRIQVVGDDFFVTNTKRLEKGIDMGA
                     CNSILIKVNQIGTVTETIECIEMAKRAGYTAVVSHRSGETEDTFIADLVVARETGQIK
                     TGSMSRTDRICKYNQLIRIEDAMEGIAEYAGKKAFYSVKK"
     gene            complement(25092..25805)
                     /locus_tag="P5313_04640"
     CDS             complement(25092..25805)
                     /locus_tag="P5313_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79684.1"
                     /translation="MENRFLRGSGPARRRVLSLAAALLLASCPAFAAGPGTTDEYRWG
                     TAPGADFRVLLEKPVVTEMEAEPWKDPETGEHRIAGWSELHGVFEVPFEALVRVLTDY
                     EGQSRYSPRLFSARVESREGDLIRVAQNVGMSILGIKVRYDLVLEYREERLSDGGFAQ
                     RSRLLSNPSGNLYESFSSWYVLPVTVDGKSCVYVRGFSRPGIRRPFPGMAGALRTFTP
                     GEVKDLLERYVKEARKLGE"
     gene            complement(25822..>26303)
                     /locus_tag="P5313_04645"
     CDS             complement(25822..>26303)
                     /locus_tag="P5313_04645"
                     /inference="COORDINATES: protein motif:HMM:NF036800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRY79685.1"
                     /translation="KVLMEAAALSQEEVAEKVGKSRPTVANALRLLKLPAEMLEALRS
                     GDLSPGHARAVLAVSSDEDRRDLFRRILAEGLSVREAEALAADLNAGKRPAPARKPEA
                     PRRPLDPDLRDLEQKLIGFLGTKVQVKGDARKGSITIEYFSLDDLERVFDLMTGGKA"
BASE COUNT         4116 a         9168 c         8847 g         4172 t
ORIGIN      
        1 cgggtctggg gcgccacagc gatgatgctg gcggagctgt tagaattgat gatcgtgaat
       61 aggtgaatcg tgaataggtg aatcggacag ccttcaccaa acactgtgca cctgctaccc
      121 gaaaacctca ttcacccatt cacttcaccc tgaacctcag caccgtcctc agcttctccg
      181 gggccgtctt ccggggatcg gacatgtaga tctcccggtg caccttggac agccgccgga
      241 gcccccgggc cgcgcagaac tcctccatcc gggcgaagct ggcgggctcc gcgtcgtagg
      301 ggccccggtg ggtcatctgg acgcagggtc cttcctcgag gacctcgaac cgggcgtctc
      361 ccaggcgggg caggggcttc cgagccttcg tccgatcgat cacggagagg gcgtactccg
      421 gggtgacgaa atccggctgg cggatcatca ggtcgaagac gtactcgctc ttggagatgc
      481 ccgctccgcc ccgggccttg gcctcctcgg agacgtccca gaccccctcc agggggaaca
      541 ccgagtattc caggtaaccc gccggagcgg tccccgactt gggggacatc cgcaccgcgt
      601 aggccagggc gtagaggacc gccatgtagt cctggaaggc gggctcgttg ggatccccgg
      661 ccccccggat cgcgaagaac ttgaacgccg gtaccgtgac gagctccggc tccgtcccgg
      721 gcaggtagaa ggccttgtcg gttttcttcc agtccagctt catgatcgat actccttgcg
      781 gcgtttccgc ggcgccccgc cctcccgcga cgggaaccgg ggcgccgcgc cccgcgctag
      841 agcggccgca cgggctcgca gatgtcgatg acgaacctgc cctccgggtg ggcgtccggc
      901 tcggacaggt agacctcgta gcacatccgg tcgtcgggct gccagcccga ggaggggaac
      961 cattccccca tcagggcgtc ccaggccgcg ccgaactggt ccggggcgat ctcgaagcgg
     1021 gccaccgcga actttccgcc cggtatggtc atgaggttga tgtccccgga gacctcggtg
     1081 ccgggcggca cggtcaggca ggcgctggag cggagcttcg cggtctcggt ggcctccggg
     1141 gagtcgtggt agaccgccag ggtccgggct cccggcaggg cgaagagccc ccggggcccc
     1201 gcccagcggg ccaggcgccc gaaggcttcg gggatcccgt cgaaggaacc cacgtggcgg
     1261 gcataggcta cggtcagttc gggcaggtcc ttgatctcga ccttgtacga aagcttcggc
     1321 attttcttcc tccttgtccc gtccgggact atgggaggag gatacccgcc cgcgtccgcc
     1381 gattctttcc ggtccttgcg gtccccttga ccgggcatgc cgagcgcttg accctgcttg
     1441 ctcccgtccc ggaggaagcg gcggccctcc cggcccaggt ccctccactc cgaggccgtc
     1501 atgccgaagc gctcccggaa ggcccgggcg aagaccgagg ggctcgaaaa tcccgaggcc
     1561 agggctatct ccgtcacgga caggccgggg ttgtccgcca ggagggccgc ggcgcgctgg
     1621 aggcggaccc gcttgaggaa gtccgagacc gtctccccca cgacccccga gaagacccgg
     1681 tggaagtgga accgagagaa ggccgcggcg tccgcgatct cgtccaggcc gatgtcgtcc
     1741 ccgaagcggg cgtagatgaa gtctatggcc cgctcgacac gccccaggta ttccctccgt
     1801 agaagctccc gccgggtcac gtcatagctc ccggatcatc tcgtactggc cgaccccggg
     1861 cccgatcccg caggccgaaa ggaggccgag ggtcgccccg tcgccggcct ggacgttcac
     1921 gtagcggatc gagggaccga gcttcgaagc gagagcccgc aggagggccg tgccgacccc
     1981 acggcgccgg tagtcccggt ccacggccag ctgccagacc gtcccggtcg atgaggcgac
     2041 gaggtggcca accgatctcc cgtcccggcg ggcttcgagc accaccaggg gctccacggc
     2101 ccgtcggacc gagtcgtcgg agttctgcca ggaaggctcc caatcccggc ggaccgcggc
     2161 ggcctttccg tccagcccct cccggcccga ctcctcgacc gttagtccca cgggggcgag
     2221 cttcccggga tccgtgaaga ccccgcctgg gcattccagg gaccgggtca cctcgaatcc
     2281 cgccttgacg taggtccgga aggcggggag gttgtcccgg atcacctcca gtacgtaacg
     2341 ccggatgccc cgggccgcga gcaggtccag ggcccgggag gccatacggc cggcaaggcc
     2401 cgagccccgg gcggcgggga ccaccccggt ccccgcgtcg taggcggcgg gcatcccgcc
     2461 ccaggtcccc cggccgttca gcacgaagcc gaccaggcgg ccgtcctccc gggcccccag
     2521 ggagatcgag aggtccacgc ccctgcgcag gcaggaagcg cggaattcct cgaagctcca
     2581 ggtcaccgga acgctgtagt ccgaaaaggc ctccatgaag acggcgtaga caacttggag
     2641 actctccgct tccagggtca tgaattcctg catatacctt cccgtatccg gggccttcgg
     2701 agtcccgacg gattcccagt atatgacagg aacctgcccg ggggaatccg cggccgcgcg
     2761 cgcacggctc tccccgtcga tggcgcccgt cgatcccgcg ggcgattccg gagccgcccc
     2821 ggggcttggc cggagtccgg gcaccgggta ctattccccc cggggggagg atacccatgc
     2881 cgaacgacgg atcgacgggc gctgatcccg gaatcgtcat catcccggta cgggacggcc
     2941 ccgggctcga ggccgcccgg accctgatcc gggagtacct ggccttcctg gacgcggatc
     3001 tctcgttcca ggacatcgac gccgagctcc ggaacctccc ggggaaatac gccccgccct
     3061 cgggcaccct gctcctggcc tccgtacata gggacccggg ccccggaaag cccgcgggct
     3121 gcggggccct ccgggacctg ggtggcggat actgcgagat gaagcggctc ttcgtccgcc
     3181 cggaataccg cgggtacggg atcggtcgtg ccctggcgga gcgactcctg gaggagggcc
     3241 gcgccctcgg ctaccgggcc atgcgcctgg acaccctgga ccgcctgggg gaggcggtgg
     3301 ctctctaccg ggccctgggc ttccggccca taccccccta ctgcgaaaat cccctgcccg
     3361 gggcgatgtt ctgggagagg gacctgtaga aggtcgaagc cgacggaacc ggcgggccgc
     3421 acgaccgaac ttctatcatt cttcgagaat cgagcgtact atctgaccat gaaacggttc
     3481 ggggcgactc tcctggtcat cctgctggcg gtcccgcgga tcctcccgca ggcacaagcc
     3541 ctcgatcccg tgcgcctcca gctcaagtgg caccatcagt tccagttcgc ggggtactac
     3601 gcggccaagg agaaggggtt ctacgcggaa gccggcctgg acgtgaccct gctggaaggc
     3661 gatcccgacg tatcctatac cgacgaagtg gtgtcgggtg ccgcggatta cggggtcaac
     3721 aacacggacc tcctgatcga ccggagcaag ggcaagccgg tggttgtcct ggctgccgtg
     3781 ttccaacact cccccaccgt cctcctgacc ctgaagggca ggggattcga ggtgccctcc
     3841 gattttgcgg gacgctcggt gatgatcgtt ccgggtgccg aggcggaagt gacctccatg
     3901 ttccggtacg aatccatccc gttggaggcc gtccggttcc ggccccactc ttgggacctg
     3961 gatgatctcc tgtccggccg agtggacgcc gtcacggcgt atatcaccaa cgaaccgatc
     4021 atcctgaaag catcgggcat agactacacc ctaatccgcc ccctcaccta cggcatcgac
     4081 ttctacgggg actgcctgtt cacgacggaa tccgaggtcc ggcggcgtcc cgggcgcgtc
     4141 aaggcgttcc tggaggccag cgtccggggc tgggactacg cgatgcacaa tcccgaggag
     4201 atctgcgacc tgatcctggc caagtactcg acccgcaaac cgagggacgt cctgctccaa
     4261 gaggccgagg ccatgcggga gctcatcctg cccgacctcg tacccatcgg attcatgaat
     4321 cccggacgat ggaagcacat cggggacacc ttcgcgtccc tgggaagcct tccggccgac
     4381 tactccctgg agggcttcct gttcgatccg cgggcgccgc gcctggaccc ccgggtgatc
     4441 caggcgggtc tggtcttcgc ggggctggcc ctgttcgcgg cgctggccta catcctggcc
     4501 ctccgatcct tcaaccgcct tctggccttc aaggtcgaga cacgaaccgc cattctccga
     4561 gagacgaacc gcctgttgga gcaggagatc ctgcgagggg cggaacggga gaaacgtctg
     4621 gccgcctcgc tctcggaaaa ggaggtgctc cttcgggaga tccaccaccg ggtcaagaac
     4681 aacctgcaga tcatcgtgag catcctcaac ctccaggccg agaagggcga cgaggtcttc
     4741 ctccgggacc tccgcagccg tgtggtgggc atggccctgg tccatgagca cctctactcc
     4801 ggcgcggatc tgggtcgggt ggacatgggt cagtacatgg gggacttggt ttcggacatc
     4861 gcggcgtcct attcccggcc ggacctctcc atccggaccg acgtgagggt tgccgacgtc
     4921 tccctctcca tcgaccaggc cgtctccctg ggactcatcg tcgcggagct cgtgtccaac
     4981 agcatgaagc acgccttcac gggtcggcga tccggttcca tcgagctcgc gctcgggcgg
     5041 gaggacggat cgtacgtcct gtcgatcggg gacgacggac cgatcgcgga gacgggtccc
     5101 cagtccgcct cggcgggctc cgccgcccgc gagggactcg gcctgcgcct cgtggaagcc
     5161 ctggccgccc agctccgggg agacctgtcg gtcgaccgga cccgggggta ccggacgagc
     5221 ttgaggtttc cggccgccct gaacgggaca taggaagcgt agggcatgtc cggagaatgc
     5281 ctaagccaaa cttcaggata atcgcgggca cccggccggc ccggggttcg gtggaggctt
     5341 gacgaatttt atgtaatatg ttacctattt ccccattgga atccaagaaa cagcccgggc
     5401 tgagagcttc gccctcctca tcgacctggg cacgaaactg caggtcctgg tggaccggca
     5461 cctcgcgccc tcgggcttca ccacgcgaca gtggttcctg tgcctcgtcc tggagcaggc
     5521 cggccggagc ctgagcctcc gggaaacagc cctgcggatg gggaccagcc accagaacgc
     5581 caagcaactg gccctcaagc tggagcgcaa gggatacctg cggatcgagc gggacccgga
     5641 ggacgcgcgc gccctccggc tctccctgac cgaagcctgc agggccttct gggcccgaag
     5701 gaaggaccgg gaccgggaaa tcctggaagc ggtcttccgg ggcttgcccg ccccggacct
     5761 cgacgccctg caccgcgccc tcaaacgtct cgatctggac gtccacaccc tgctggcgga
     5821 cgacagcctt ccgttcttat aaggagaaat catgaagccc gtcacgaaca cccagaaggc
     5881 tttagccctg ggcatcgccg tcctctcgct cttcgcctgc ctggcgggtc tggccccggg
     5941 aaccccgcgg ccggattccc cggccgcctt cacctcgatc cggggtgagc gggtggaact
     6001 ccaaggcacg gggctctacc ggctagattc ggtatccttc gccgcccagg ccaaagccca
     6061 ggacctggtg acccttttgt tgggaatccc cctcctgggg atctccctgg tcctcgccgt
     6121 ccggggcagt ctgagggcgg gggtcctgct ggctggcacc ttcggatatt ttacctatac
     6181 ctacgcgacc tacgccttcg ggctccagta caatcccctc ttcctggtct acatcgcgct
     6241 tttcgggtca tccgtcttcg gactgatcct ttccctggcc ggcctggaca ccgtgaggat
     6301 ccgggaggga ttccggggcc cccgggtacg gaaggcggcc gtcggcttcg acttcctcgc
     6361 gggaaccctg atcttcctca tgtggatggc gcggctcctg cccaacatcc tggccggtac
     6421 ggacaccacc ttcatcgagc actacacgac cctgcccatc caggtgatgg acctggggtt
     6481 cgtcgtcccc ctggccctgg tctccggagt gaatctggcc cgggaccggc ccctggggta
     6541 cctgctgacc gggctcttcc tcatgaaggg cgtcaccctg ggcttggcgg tcggagccat
     6601 gatcctgtgg tccgtcgtcg cgggcctccc ggtctctccc gcggaaaccg cggtcttcgc
     6661 ggtgatcatc ggggcggggg tcggcctggg cgcggcctgg ctgggggcga tccggtagaa
     6721 ttccccggcg gggatccata atcctcccga tcggattcga atcccgtccc tccccgcgct
     6781 acactctccg catgcgcaat cgtctacacc gcggccggat cgccgcggcc ggcttggcgg
     6841 cctccctcct gggggccttc ggtctcgccg cctgcgtagc cccggcgccg cgaggcggcc
     6901 cttcgaccgc ggaggaggcc gtcgctacgg ccgacggagc ctggatcgcc gcccgggacg
     6961 gggtcctcct gcgggtccac gcctgggaac cggccggcgc cgcccggggg atcctgctcc
     7021 tggtgcccgg ggtcacgggc ctggataccc ggatggaagc gcccgtgacg gaacccctgg
     7081 cggccgcggg cttcgcggtc ttcgccctgg aaccccgggg gaccggggag tccgggggcc
     7141 cccggggcta cgccggaaac gcttccctca tcctggacga cctgggcagc ttcgccgcct
     7201 ggggccggga acgggccggc caaggcggaa ccgcggaggg cctcccggtc ttcctcttcg
     7261 gccacagcat gggggggacc ttcgccctgg ccctggccgc ggagatggag acgccgcccg
     7321 cgggcctcgt cctggtcaac ccggcgtaca agtaccggcc cctcaaggga gccacccccg
     7381 gcttcgggga ctacctgcgc ttcgcggtcc attggatctt cgccccccgg aagcccgtgg
     7441 tggatatggg gggagatccg tccctcctcg aggatcccga cgaccgggcg gaggcggttg
     7501 agcggaaggc cagccccgcg gtccagggct tccagtccat ggcctccatg accgcggccc
     7561 ggaagatcat gaccgcggcg ccccggaacg ccgcccgcct ctccgccccg ggcctgatcg
     7621 tggagggaaa gcgggacctc ctgatcgacc ccgcgggcac ggacgagatc tccgcggcct
     7681 ggggcggccg gggtgccgag cggatccggg tggatggggc ccacggccgg agctcggccc
     7741 tggccgggat ggaaacgatt ctcaggtggc tggcggagga ggcgggacga tgaggggcac
     7801 ggagctggac gggaaggtgg ccgtggtcac gggaggcggg tccgggatcg gccgggccct
     7861 ctgcggggag ctggcccggc ggggagcccg ggtggtcgac acggcggggg ccgaggagac
     7921 cgcggccggt atccgggccg gggccgccgg gccggaggcc gggccgaggg cgctcgcacg
     7981 ccgggtggac gtcacagacc ccggggacgt ggaagccctg atggcggaga ccgtccggga
     8041 tttcggccgg atcgatttcc tcttcaacaa cgccggggta tccgccaacg gggagttcca
     8101 ggacatcggc ccggagcact ggaagcggat cctggacgtg aacctctggg gggtcgtcca
     8161 cgggtgccgg gcggcctacc cccgcatggt ccgccagggc ttcggccaca tcgtcaacac
     8221 tgcctccctg gcggggctca tacccggcag cctcacatcc gcctattccg ccagcaagca
     8281 cgccgtggtg ggcctgtccc tgaccctccg cgccgaggcc cgaacccggg gcgtccgggt
     8341 gaccgccctc tgcccgggct acatccggac ccccatcctc aagacgaccc cgaccttgag
     8401 cccctacatg gattccccga ggaaccggga gatgaacgag ggcctgcgct tccccggccc
     8461 cgaaggctgc gccccggcca tggtccgggg cgtcctgcgg aacaaggccg tcgtgatcgc
     8521 gcccgcatcc caggcgatct actggctgct ctaccgattg gccccggccc tcctgccctg
     8581 ggcgggctcc aagatcgtgg agacgatgcg gaggaacgcc gaggcggacg ggaggtagcg
     8641 ttccggcggg gatgaaccgg gtttccctcc gatccgggaa aggatcgggc atcccccctg
     8701 gagcacggaa tgctccgccg gtccccgccg cccatcggac ctatctcccc ccggacatat
     8761 cgtaccagtc gttcaccggc gtcttctggg tttcgacgaa ccgggcgccc tggggcagga
     8821 agtacttgag gccgagggtg aaggcgaaac gtccccggac ctcgtcgagc aggggcactc
     8881 cgggatcgta gggatccccg aaggcgtcgc ggacccgccg gatgacgccc agacggaatc
     8941 cggcctccgc gtagggggcc agaagctccg acacccggta atacgcgcgg accttgggct
     9001 ccagccagag ttggaacgac agactgtgat cggattcccg gacgtcgtcg gacaccccgg
     9061 gaaggccggg ccagatcccg actcccagtt ccgccccaac gcccaggatc caggagctcc
     9121 ccagggggaa gtaccaggag ggctcgaagc cgagtcccag gaacaggtca cggctgatac
     9181 tttcctgcag gctggagttc agagtatgcc gggagtacag gagcatcgac gggcggaggg
     9241 ccagggagaa ccggtcggcc aggaaccggc cgacgcttat ggacgcctcc gtgatctgga
     9301 gatactctcc cttctcctcc tccgtgacat ctcccgcgaa aatggggtag tcgggattat
     9361 gggagaactt gaccgtgccc ccgaactccc agccccccgc ggccagggga tcgggcgcct
     9421 gggcgaaggc cgcggcggaa accgcgatca gggccaggac catccaaaga cctttgttgc
     9481 gcatacaacc tccgcatgaa tgatgggatc gagatcgttc ccacatcctg cgggggatgt
     9541 tcgagaaagg ccatcacccg aaaggggtta ggccgagggg ctcagcccgc ggcgggagga
     9601 cgccggggca atcacggcga tcccgccgct acatcaccgg cacgacacct cgttcgagta
     9661 cgcggaatac tgcaggaccc cgccgccgat gtcccggtat ccccggaccc ggtatttgta
     9721 cgccttcgtg ttatccggcc gggtatcggt atgatccaat acccattggc ttacattcca
     9781 gctcttggct ccgatcagag cggaaccgac ctccgaccac agcccggtat cggggttgta
     9841 tcgctccgcc ttgtacccgt ggactccccc gtccgacagc tggtatatcc gtaccgtcga
     9901 ctgccagggc gcggccatgg tgtaggagta aagctgcagc gcgggagtcc cgatctccgt
     9961 ccgcgccgtc gtcgtcgcgg gagcggacgt gcctccggcg tttcgggcgg tgatgcggaa
    10021 cgcgtactcg cccaaggact cggggatcca gaccaccgtg ccggtcttgc cggacggata
    10081 gtactgggcg aaggagatgt ttgcctgtct ggatccgggg atgccggtgg ccccgacgac
    10141 atacctccac gcgccgtcga cgaacacttc gatgtcgaac catcccaggt tgatcccgct
    10201 gtgcgaccat gccgcgggat cgatgtcgta tctccacccc gccgaatccc agcctcgtgc
    10261 ggccagattc acgctgaagc ctgtcggggc cgccggcgga ttcaccgtat acgtttggga
    10321 cgcgatgtcg ctgttcgccc agccgggccg ggtcgccatg accttcacgg tcacgggcga
    10381 catatccacg gaataggaac cgccggacgc ggaggtcccc gtcgggtcgg acccgtcgac
    10441 ggtgtaccgg tatgtcactc cggaggtcgc gcatccgaac gtgaccgcgg cggcgtggga
    10501 cacgatcccg gatccgggcg cgagggtggg agcggcgacc ttgggagtat aggcggcggt
    10561 ggcgacgccg ctcgaggtcc acccggcctt cacggcgatg accttcaggg tgaccggatc
    10621 gttgacctgg atgcccgtcg cgctcgggga acccgtcgtc ggcgtcgttc cgttcgtcgt
    10681 gtagtggtag gtcactcccg cgacaggact ggaaaagctc accgtcgatc cgccctccac
    10741 ggtacccgcc gccgggctca gcccgggagc cggcagccgc ggagcgtagg tcgcgaccgc
    10801 gacggggctg tccgccatgc ccgccttcac cgccagcagc ttcagcgtca cgggcccgtc
    10861 gacctggtag gacgaaacgg agggggagcc cgtcgtcgga tcggtcccgt ccgtcgtgca
    10921 gtggtacgtc accccgctcg tggcgcagga aaacgtgacg accgtgcccg ggtcgacagc
    10981 gcccggcgcc ggggagaact gcggcgtaga aacctggctg taatcgatgg tgtaggtcgc
    11041 gtccgccgcg tcactgtcgc tcatcccgga catgcgggcg aaggcgcgga tcgtcatcgt
    11101 ggtgccgttc ccggcgatcg cgatcggtac ggtgtacagg gtcgaggatc cgccgggagc
    11161 cgagccgtcc gtggtgtacc ggatctcggc tccgccggta gcggaggaga tttcgaccga
    11221 ctggtcctcc gagaaggttc cggggctcgg agtgaaggtg ggtgcggcga cctgctcgag
    11281 ggtgggatcc tctccacccg cgcccgccgc ggcgcacccc gccagcatcc cggcggcggc
    11341 taggaaggcg aggacggcga aattccggaa tcgcatgggt acctccgatg attatggatc
    11401 gacgggtacc cgatcctagg catggaagta ttggacgcgc catcacccga acagggttag
    11461 gcctcggccg cacgctcctc ggccgcggac ctccggtccg gctccaggca gagctcgatg
    11521 aggcccttgc ggccgggcac gtcgaatttg cggtagacgc tcttggcgtg tgtcttgagg
    11581 gtgttcaccg aaatgtccag cctccgggcg atgctcccgt agtcgagccg ccccgccagg
    11641 agggccgcga tctcgatctc ccgttccgtg aggcggtacg gggggtcgaa gcgctcgttg
    11701 atcctccgga tcttgcggcc catcggttcc cggatgtacg cgggatccag gatcgcgacg
    11761 tggccctcct cctcgtactc cggagacgac ggggcatccg cccgcgaacc gggcggcccg
    11821 atccgcgtaa ggcttcggag ggagagttcc tcctcgagca cccggagcaa ggggggcagc
    11881 aaggccaggg ccgtagaata gaagacgacc atcatcccga cgaacgaggc ggtatcccga
    11941 cggcccgaca gctccaggac ccacccgatc aacatcccca cggacgtccc caggactacc
    12001 gccccggtga agagtatcac gatgagggga cgaccccggt gcttatggaa gatgtcgaag
    12061 gtcagcttga acagaaagag gttccccaag gcctccccga cgaccaggac cagggagccc
    12121 gccgaggtgc cggcgtgcaa gatgatgagc ccgtacccga tgagctggca aaggatcatg
    12181 gaatagagca ggaggaggat gtggatgccg cttcctttcc ggaggaacaa caggtagacc
    12241 gcggaagaga cgaggaccct gacgcccgga gacggcgtcg tccaggcgct cccgcccgtc
    12301 acgctcagga gtccctcggg caggaaggtc gcgaaataca cgagcaggag caggacgatc
    12361 acgatcccca tgagccgccc cgggaacgtc agcggtggac cgctcccggg atcctccacc
    12421 cggctcatgt ccacgcgaag ggccgcccaa agggcgacca ggggcaccgc gaggtccatc
    12481 gccgcggcgc ccggagtgcc gaaccgctcc cccgcccaca ggaccgcgag ggacacggct
    12541 ccccctccca ggtgcaccgc cgcgatggag gagaccctcg gaagccccgg gaatgcgtac
    12601 acccaaaaat ggaacatggc gccgaaggag gtaccggcca ggatcccgaa gagaccgaat
    12661 ccacatccgc ccgccctcgc cggcggtatg aaccggagga ccaggatcgc cgccagggcc
    12721 gcagccgcgc aaccggaaac cattgccttg acgttgaagc ggcgcatgaa ggatagcccg
    12781 gcgaacatac cgatcagaaa tccgccgaaa tagaataggc gggcggagtc cgcgccgccg
    12841 tccggacccg tcaggatgat ccacggggac gattgataga agtacgccag ggtccagccg
    12901 aacagtaggg agcaggtcag gaaggcggac gggaggaagg gtcggcgggt ctccgcggag
    12961 gatccgttgg ttcgattaca taattcgttt atagctgaag ccatgctgca ggttcgactg
    13021 tattccaggc gccgcctcct gtcaacgcga gggagaggat acccgcccgc tgtagccccc
    13081 caggatcagg gaatacccac gctcgatgtt gcggcggcag gagccctcgt ccatgtcgtg
    13141 ccggccggaa aggaaggcca ggatgagacc gttcgccaga accaggaaga ggaagccgtc
    13201 cctccggagc tcctcctggc ggacgggatc caggagcccc gggatcaggt cgatcccgct
    13261 ccagtagagc ctccgcacct tgggatcccc ggtggtcggt ccgaacccgc tctggaagac
    13321 gaagcggtag aggttgggcc ggcgcttcgc gtactcgagg agctcctccg cgatgatctt
    13381 gaacaaggcg caaggccggc acggtccttc gacttcctcc ggaatccggg gtttgagagc
    13441 ctccgtgatc tcctcgaaac cccgggcgat cacaccgtcc aggatctccc caaaggccag
    13501 gtaattgtag agggtcatgt gggagacccc gagtatctgg gccagccgcc gggccgagaa
    13561 gtcctcgtac ccctcccggt cgattaggtc gtaggcggtt tgcacgatgt ccccggggtc
    13621 cgcgacccgt ttcgatcttc ccattctgcg ttcgatagta ccctgtcggg ccgccccggc
    13681 caagcccgga cggcgggtga cgactccggg gcgagggacg tcacgagact gagtaccccg
    13741 tggacaggac agcgttccga ccggcctcca tgccgaacac caggggggcg tggtacccga
    13801 acctatcgcc ggggagcccg aagaccacgg ccttgccctc cccgcccctc cgtgccacaa
    13861 tgcccccggc aggaggatca tgttccaccc caagacgacg gactgggaca accggatgaa
    13921 ggagatgttc gaccgcatcg actccatcct ggaggaccgg taccatggat cctggaggct
    13981 ccggcgcaac cgccccggac gcggcgaaac ctcgaatccc gaggcggacg gtctctttaa
    14041 cgtgggggtc ttcttcaccc ccgggttcgg gagccgcctg ggccggggct acctggtgga
    14101 gatcgtcctg gccacggagg aggccgttcc ccgggccaag cgggacgaga tcgaattgtc
    14161 cgtccgggac ctgctcctgg aattcctgcc cgtctacttc cccgaccgaa ggttgaccgt
    14221 ggagcgggac ggctcgatgt acaagatccg gggggacttg ggcctgggcg gggtttagcc
    14281 ttcgaaggac caactcgaag ccttgatttc cccggccgcg cactatactg gaaccatgac
    14341 catctcggac ttcctatcga tgaggtggag cgggatcgag ggccccctga tcgagcctcc
    14401 ccggctctcc cccatcgtgg cggaccctag cttcctgttc ccggaggaga ctcccggcgg
    14461 ggtctgggaa ctcttcgccc actcggcctg ggggatccat cggtatacct ccccggacgg
    14521 cgcgagatgg accgaacggg gcatcgccgt ccacaacgcc atgcgcccct tcgtgcgccg
    14581 cctggacggg gaagggctgt gtccggacga ggggccggcg accgggagcg gccgccgtgg
    14641 cgccgccccg acggccgccg gtcccgcggg aaagccggca tcctcccgtt tcggcctgta
    14701 ctacgagtcc tacccgcctc tggccctgcc cctgaccgcc ctgccgttcc gccggcgctg
    14761 gaggtcccgg atcgccgtag ccgtgagcag cgacctggag cggtggtccc ccggccggac
    14821 cctggtctcc cccctccttc cttggatgcg ggaccgatct ctgggattcg cggtgtccaa
    14881 tccctgcctg gtacgggact ggaccgacac gtatcggctc tacttctccg cgtccctggc
    14941 ctggatcgag gactgcggct tctgcgagcc gaaatatgtc ggcctggccc ggggagcctc
    15001 cccggagggt cccttcgtcc cggatcccga acccgccgtc gaccccaccc gggatccgat
    15061 gcccggagtc cagggcgccg gggcgatcaa ggtcctgccc ctgtcggacg gctacgtggg
    15121 actccagaac aaaatctacc gggatccgtc cgggcgctcc cgctcggccc tcttcgtcct
    15181 gcgctccgag gacggcgtct cctgggaacc cgcccgacgg gaacctctcc tggccccggg
    15241 ctccggttgg acggcctccc acgtctacgc ctgcgactgc cgtttccggg aatccgacgg
    15301 ccgctggtac ctctacttca acgcccggga cggatggcgg atcagcgaag gccgggagcg
    15361 gatcggacgg atcgtcggga ctccgggctg agcggccggc ccgagtccgg gctcggcggc
    15421 ccggaggccc cgccgcggcg gacaccgcgg cggcggcttg cgggcccggc ccggccgtcg
    15481 tatcctgagg gaccatgccg gatccgacgc cccccgccgt cctcgtggcg gacgacgacg
    15541 acaacctccg cgcctccgtc cgcgcctccc tggagaggga aggcttcggt gtacgggagg
    15601 cccgggacgg ggtcgaggcc cgggacttcc tggccggagc cccggatccg gccggacggc
    15661 cggccctggt tatcctggac atctccatgc cccggctttc cggcctggac ctcctgcggg
    15721 agctccgcac ccggggcgac acgatacccg tcatcttcct gacctcccgg gacgaggagc
    15781 tcgaccgggt cctgggcctg gaactggggg cggacgacta cctccccaag cccttctccc
    15841 tgcgggagtt ggccgcccgg gtacgggccg tgctccggcg ggtcgagtcc cggtccgccc
    15901 cggccgccgc ggatcccccg gcggccggaa cccgggaatc ccccgccccg ggctccctca
    15961 ccctggaccg ggacgggttc cgggccttct gggccggtac cgaactggac ctgacggtca
    16021 cggagttccg tatcctggac tgccttgcgg agcgtccggg cgcggcccgg acccgggact
    16081 ccatagtcgc ccgggcctgg cccgggggcg tgtacctctc ggaccgggtg gtggactgcc
    16141 acgtcaagcg actccggcgc aagctccggg aagcgggggc cggggacgag gccatcgaga
    16201 cggtctacgg cctagggtac cggctgcgcc catgaggcgg ccccgggttc ccatcgttgc
    16261 ccgcatcctg ggggtgaacg ccctcctcgt cttcctgccc gtggcggcct tcctctccct
    16321 ggacacctac gagcggagcc tcctgtcggc cctggaggat tccctggtcc agcagggccg
    16381 gttcctggcc gcctggctgg gggagggcga gctttcggcc tctcgggcgg aggccgtgct
    16441 cgccgccctt ggccggcgcc ataccgcccg gctccgggtg ctggacgccg cggggaacct
    16501 cctggcggac tccagcgtcc gccccccgga cgcgcccccc tccgccccgg acgcggaggc
    16561 cccgtaccgg gcgggaccgg acgtcctccc cgaggccaag cccgcggaat ccagctggat
    16621 ctaccgtctc ctctcgggcc ccgtccggct ctggcggcgc tacctggccc cgccctccga
    16681 gcccctggcc tccgcggact tctacacggg caagcggaac ttcctggagg gagccgaagt
    16741 ccgggcggcc ctggaggggc gctacggcgc ggcgacccgg atctccgcgg gaggccaggt
    16801 ttcggtgacc ctgtacagcg ccctgcccgt cccgtcgccg gacgggacac cccgaggggc
    16861 ggtcctggcc tcccagtcga cctaccgcat cctggcgggc ctgtaccggc tccggctgga
    16921 cgtcggccgg atcttcctgg ccggcctggc ggcctcctcg gccctgagcc tccttctgtg
    16981 gctgacgatc tcccggcccc tgaggcgcct gggaatccag gcccgggccg ccctcagtct
    17041 ccggggcctt gcggaaggcc atttcaagcc cggcgccgtt tccgacgaga tcggggacct
    17101 gggctcggcg ctgcgggaat tctccgcccg cctgggggag cggctctcct gggcggagga
    17161 cttcgcctcg gacctgtccc acgagatccg caatcccctg gcctcgatcc gggcggccgc
    17221 ggaactgctc ccggacgccg ggccggagga ggtccggata ctgggggatc gcatagcccg
    17281 ggacgcggac cgggcggccc ggatcgtggg aggcctgcgg gagctctccc ggatcgaggg
    17341 aggggaggac gggaccgggc ccgcggacgt cctggcggtc gcccggaact gcgcgcgctt
    17401 cgccgcggac cgctccgggt ccgggtccgg gccgacggac acgaggatca ccgtggaagc
    17461 cgccccggat gcgggggaac tccgggccgc cgtctccccg gaccgcctgg ccctggccct
    17521 gggcgccctc ctggacaacg cggtgtcctt cagccccccg ggcggggagg tcgaggtccg
    17581 ggcggaccgg actcgggaag ccgacggcga gaaaatacgc ctgtccgtcc tggaccgggg
    17641 accgggcata cccccggagc accgggaccg gatcttcgac cggttcttca ccttccgccc
    17701 ggccggccgg gacgggaacg tccccggcac ccgggccgcc gaacaacaag cttcggaaca
    17761 ccaggggctc ggcctggccc tggtccgggg gatcgccctc cgggccggag gccgggtcga
    17821 gaccgccgac cggcccgggg gcgggacggt cttcaccctg gtcctgcccg cggcgccccg
    17881 ataacacccc gccgccgcca cagttccgcc gcattcccgg cctagaatct ccgggcaagg
    17941 agtgcgccat gaacagaaca ccgagattgc cccgagccgc ccagacgagc ctcaaggccg
    18001 gcctggtcgg cgtcctggtc ctcgcgttcc tcatccccgt cggcatgatc cgggaggtcc
    18061 ggtccgagcg ggaatcctac cgggagaacg ccctggagga cgtctcgtcc aaggcggggg
    18121 gccggaccct tttgatcacc ccctacctgg ccgtgcccgc acgttacgac gtccgggaga
    18181 cgaacgccaa gggagaggag gtagtccgta cccgaaaagc ggtctacatc gtcttccccc
    18241 gaaccctcgg gctcacctgc gacacccggg tcgaactccg gaagcgcggc atctactcgg
    18301 tgcccgtcca ccggaccgaa gcggcgatgg atctcgagtt cgatctccgt tcctgggaaa
    18361 cggggatacc cggcgtccgg gtgggctgga ccgaggccca gctctactac gcctacgacg
    18421 atccccggtc cctgcgaggg agtccctccc tggcggcgac ggacggttcg agggcggtcc
    18481 tgaggtcgag cccggccccg ctcgagctgg ccgcccgatc cgtctccgtc cccgtacgcc
    18541 tccagcccgg gaccgacgga agcgcgcggt acgcggcgcg cctggaactc tccttgaacg
    18601 gggcggaagc cctggacttc ctggccctgg cggacacgaa caccctgagg ctttcctgcg
    18661 actgggccag cccctccttc tcgggctacc ggcttcccgc cgaccgggaa ctgggggacg
    18721 cgggcttccg ggccgaatgg ttcgtggacg agccctcccg ggtcctgccc cgggtcctgg
    18781 ccgcctcgga tttccggccc gaatcggtcg cgggggcggc cttcggggtc gacttcctcc
    18841 aacccacgga cgtgtaccag caggtccacc gggccctccg gtacgcggtg ctcttcctct
    18901 tcataccctt cgcggccctc ttcctcttcg aggtcctggc tcgccgccgc ctccacgtgg
    18961 tccagtacgt cctagtgggc ctggccaact gcctcttcta cctcctgctc ctggccctgg
    19021 cggagcacct gcccttcctg gccgcctacc tggtttccgc cgcagcggtc tgcggggtca
    19081 ccaccttcta tgtcggcgcc ttcctcccgg acaaacgcca ggccgccctg gccttcgccg
    19141 tcctggccct ccagtacggc tacctcttct gcgccctgtc ctccgaggat tacgccctgc
    19201 tcatcgggtc cgtggggctc ttcgggatca cggccctgac catggcggcc acccggaaag
    19261 tggactggtt cggcggcaag ggggaagcgg taccggaacc ggacggggaa ccgcaggatc
    19321 tgccggaatt cccgcccctt cgggagccct gaacgcgaat tccgccgggc gcggggcccc
    19381 ggttccgcgc cagagtggcc tcatcgagac accaaggagg tctgtatggc ggagacgatg
    19441 gttctgcggg aagtcggcgc ggtggaacgc cgggacgggc ggacctgggt gaggatccgg
    19501 ccggaatacc gggaagcctt gcggggcttg gaggagttca gccactgcca cgtcctgtgg
    19561 tggtcgggaa gcgacttcgg gatgggcttc gacacccggt cggtgctgtc cggggaactg
    19621 ccctactccc cgggcaggac cgcgggggtc ttcggctgcc ggggaccctt ccgacccaac
    19681 ctgatcgcga tgaccgtctg cccgatccgg gccgtggatc ccgaggccgg ccggatcgag
    19741 gtcgccgcga tcgacgcctt cgacgggacg gcggtgctgg acgtgaaacc ctactacccg
    19801 agcgtggacc gggtccggga cgcccgggtc cccgcttggc tcccggactg gggggagtgg
    19861 gctcccgacg gcgggttcgg gctggacgag tagggccgcc cgccgcatac tggcgtacat
    19921 ggacaacgcc caggtgatcc tggagaccgc ccgctggatc gaggggcggc ttcgggagga
    19981 cctgcccgtg cccgcgatcg cggccaaggc gggctactcc ctgcaccact tcacccggct
    20041 cttcgcggcg gtgatcggca tgccgcccaa ggaatacgtc ctgcggcgca agctatccga
    20101 ggccgcccgg gacctggccg gaggccggcg cgtcaccgac acggccttcg actggggctt
    20161 ccgggacctg gacagcttct cccgggcctt ccgccgggaa ctcggcagtt cacccagcgc
    20221 cgtccggcgg ggcgccccct tcccctatca ggagcccgcc gtcagacgga tccccggtcc
    20281 ccccgtggac gcggagcccg tcctggagcg ctttcccccg gtcctgctgg cgggttggca
    20341 ggtccgcatc acgggtccgg gggacgaggt gggacgcctg tgggcgggct tcctgccccg
    20401 ggcggcctcg attaaccgcc gtatccgacc gccgcgggtc cgccagctgg ccacctggac
    20461 cgaggacgcc gaggactggg tggacatcct cgtggccgcg gagatggaaa ccctggacgc
    20521 cctgcccctg gacctggtcg gccgggcggt cccggggtgc gagtgccttt ccttcctcca
    20581 ccggggctcc gccgcccggg tcggggaaag ctacgcggcc atctacggca ccctccttcc
    20641 ctccctggac cggaagccct ccctgccgtt caacttcgaa tggtacttcg aggacgcggg
    20701 ggatccctac tccgaggact ttcgcttcaa gatctgcgta cccgtggcct gagagggaag
    20761 cccgaccaaa cctgtactta tcccccctcc cgtggtaaaa agggggaaag gaggcaggca
    20821 tgggattctt caagaagatc cgaacgacgg ccgtcaaggc cgtccgggcc ctgacccggc
    20881 gggcctccac ggtcctgggt tccctgcgaa agtcatcctc ccccctggcg gatcgcctcc
    20941 tttccgacgc cctccggatc gcggagatcc cctccccgac cgcccgggag gaacagcggg
    21001 cggccttcgt cctggaacgc ctgcgttccc tgggactgaa cccccaggtg gacgaggacg
    21061 gaaacgtcct ggcccggatc acatgcccgg tcccggacga cccccggccc atcctggtcc
    21121 tggcggagct ttcctcaccg cgctggcacc ccctggaaag cctctcccgt ctggacgcgg
    21181 accgggccta cggcgcgggg ctttccgact ccctgggacc ggccaccctc ctatccctgg
    21241 cggagtccct gcagtccggg accccggcca tccccaagga cgtcctgctc ctcttcgcgg
    21301 cccgccccct ggacgatccc cgggggtcgg tgctccggcg cctggccgag gatccccagg
    21361 accggcccgc cgcgtccctg agcctccggg ggctctccct cgggaccgta ccgaccaagt
    21421 ccctggggac ctaccgcata gaggtccggg tgcgcaccga cgcggaggta tctgagacgg
    21481 atcgcgggcc taggaccgcg gtggacgcgg tcgtagccct ggcgcggacc ctgacgggca
    21541 taacctggga cgcggaaaaa aggacaacct gcgcgatccg ccgtatcgag gcgggttcgg
    21601 gcttcggcca cgaaccgacg gagggactcc tggacatcga actcgagtcc gccgacgagg
    21661 cggtcctgga gatcgcgatg aaggccgcca cggccacggc ggagtcgagg ggccgggagg
    21721 cgaacctcca gacccaggtg acgatcgccg gctacgtgcc cgtgggcgat cccggggccg
    21781 gggcgggact ctcgaagctc gtgatccagg ccctgaagga gcagcatatc aaggtgcggg
    21841 aagagtccgg agccgacccg gcggccttct tctcgtcccg cgggatcccc gccgtatccc
    21901 tggcggtggc cagcggccgg gagggcctgg accgggacga gatcgaggtg gcctccatcg
    21961 agaagggccg ccgcctcctg gtctcggtga tcgagcgggt ctccaaggag gtcctgtgag
    22021 caagccttcc gacgattccc tcctggtgcg gacctggcac cactcgaagt tctcggacat
    22081 aggccgtctg gtggatctca aggagaagaa aggcctgacc ctatccctgg ccttcccgac
    22141 cctgaacgag gaggcgacga tcggcaagga gatcctggtc atccgcacgg agctgatgga
    22201 ccgatacccc ctgctggacg agatcgccgt catcgactcc tcctccaagg accggacccg
    22261 gcaggtggcc gagcgctacg gggcccgggt cttcgagtcc aaggacatcc tgcccggccg
    22321 ggggaccttc cgcggcaagg gcgagaatct ctggaagagc ctctacgccc tacaggggga
    22381 catcatcgtc tgggtggacg cggacatcgc caacatagcc ccgaaattcg tgtacggcct
    22441 cgtgggcccc ctgctggagg acgactcgat cgggtacgtg aaggccttct acgagcggcc
    22501 catccgctcc agctcgggga tccagtcctc cgggggaggc cgggtcacgg agatcctggt
    22561 ccggcccatc ttctccctct tctacccgga gctggcgcgc ctagtacagc ccctgtccgg
    22621 ggagtacgcg ggccggcggg aactcctgga gaccctgccc ttcagcgtag gctacggggt
    22681 ggagctgggg cacctgatcg acgtctaccg gaagttcggg atcggggccc tggcccaggt
    22741 ggatctggac cttcgcatcc accgcaacca gagcaccgag gccctgggac gcatggccta
    22801 cggcatcctg gccaccttcc tggaccgggc ccggcgctac ggcgacgccg aggtgctccg
    22861 cgagctgggg gaccgtcaca tcgcactcaa ggtggagaag gaatcccata ccgtcgcggc
    22921 cacggagatc tccgccgtgg agcgccctcc catggtcgag gtccccgagt acacgcagaa
    22981 gttcaaggag tccggacggc actgggctgg gcggaacctg gaggcctagc gagccgtggc
    23041 gccggcgccc cgcacgggtg tcgggtctcg gattgcgggg agccgcccgg gggtatatat
    23101 tcaaggggtg agcgaagaca cgatcctgga atccagcccc acgggcaagc gtctccagtc
    23161 cctccgcaag gacgataccc tttcgatcct cctgtgcggc aagcggattc ccctgatcgg
    23221 gaagatcgtg ctgggccggg accgctcctg cgggatcgtc ctggagcacg ccctggtcag
    23281 caaacgccac gcgatgatcc agaagatcaa ggacgactac ttcatcaccg acctgtccag
    23341 caccaacggg accttcgtca acggaaaggc cgtccctccg ggcaagtacg tgcgcgtcga
    23401 gccgggggac aagatcaaga tcgggaagac ggtgctgacc ataaaatagc cgcgaatccc
    23461 cgtacgacgg cgcgacttcc acgtgaaaat gaaacggccg cccccatcgg aggcggccgc
    23521 cgcgtccgaa gccggacagc ttacttcttc acgctgtaaa aagctttctt ccctgcgtat
    23581 tccgcgatgc cctccatggc gtcctcgatc cgtatgagct ggttgtactt gcagatccgg
    23641 tcggtccggg acatggagcc ggtcttgatc tggccggtct cccgggccac cacgaggtcc
    23701 gcgatgaagg tatcctcggt ctccccggag cggtgggaga cgacggcggt gtagccggcg
    23761 cgcttggcca tctcgatgca ctcgatggtc tcggtgaccg tgccgatctg gttcaccttg
    23821 atcaggatgg agttgcaggc ccccatgtcg atgcccttct ccaggcgctt ggtgttggtc
    23881 acgaagaaat cgtccccgac cacctggatc ctgccgccca gggccttggt catcttcacg
    23941 tagccgtccc agtcgttctg gtccaggggg tcctcgatgg agacgatggg gtacttgccg
    24001 acccacttgg tgtagatctc gatcatctcg tccgcggaga agagcttgcc ggggttggac
    24061 ttccagaact tgtacccctt cttctccccc tcgtcgaaga gttcgctgga ggcgcagtcc
    24121 agggcgatgg cgatgtcgtc cccgggcttg tagccggcct tctcgatggc cttcatgatg
    24181 tagtccaggg attcctcgtt gccgatgttc ggggcgaagc cgccctcgtc gcccacggag
    24241 gtgttgtgcc cgtccgcctt gaggaggccc ttcaggctgt ggaagacctc ggcggtcatc
    24301 cggacggcct cccggaagga ctgggcgccc acgggcatga ccatgaactc ctggaagtcc
    24361 accttgttgt ccgcgtgctt gccgccgttg atgatgttgg ccatgggcac gggaaggagg
    24421 gtggtatgcg cgccgcccag gtacttgtac aggggggtct cgagatagtt ggcggccgcc
    24481 cgggcgcagg ccatggacac gcccaggatg gcgttggctc ccagcttgcc cttgttctcc
    24541 gtcccgtcca ggtcgatcat ggtacggtcg atgtccacct gctcggtggc gtccagtccg
    24601 atgatttccc cggagatgac gttgttcacg ttctcaacgg ccttgagcac gcccttgccc
    24661 aggtagcgct tcttgtcgcc gtcccggagc tccacggcct cgtgctcgcc ggtgctggcg
    24721 ccggagggaa cggccgcacg gccgacggtg ccgtcctcaa ggacgacgtc cacctcgacg
    24781 gtggggttgc cgcgggaatc caggatctct cgggcctcga cgtattcgat cacgctcatg
    24841 gtacgctcct cgggatatag ggggatgggg cggccggccc gtagggcccg cccggtgctt
    24901 gtctacgaag aaagcctagc aactaagggg aggacagtca actgcgagag gagtgaatag
    24961 gtaaatcgtg aatgggtgaa tcggagggaa agcgggcttc ggccgagtcc gctgcttttc
    25021 aataaccgct ctccaagctc cggcccccgt cttattcacc cattcaccca ttcactcatt
    25081 cacccattca cctattcacc taacttacgc gcttctttta cataccgctc cagcagatcc
    25141 ttcacctccc ccggagtgaa ggtccgcagg gccccggcca tgccggggaa gggcctgcgg
    25201 attcccggcc gggagaatcc gcggacgtag acgcaggact tcccgtccac ggtcacgggc
    25261 agcacgtacc aggaggagaa ggactcgtac aggttgccgg acgggttgga gagcaggcgg
    25321 gagcgctggg cgaatccccc gtccgaaagg cgttcctccc ggtactccag gaccaggtca
    25381 taccggacct tgatgcccag gatggacatt cccacgttct gggcaacccg tatgaggtct
    25441 ccctcccggg actccacccg ggcggagaag agccgcgggg agtaccggct ctggccctcg
    25501 tagtccgtca ggacgcggac cagggcctcg aagggcacct cgaagactcc gtggagctcg
    25561 gaccacccgg ctatgcggtg ttccccggtt tccgggtcct tccagggctc ggcctccatc
    25621 tccgtgacca cgggtttctc caggaggaca cggaaatccg cccccggggc cgttccccag
    25681 cggtactcgt cggtcgtacc ggggccggcg gcgaaagcgg ggcaggaagc caggaggagg
    25741 gcggcggcga gggacaggac ccggcggcgg gcggggccgg aaccacgaag aaagcggttt
    25801 tccatgaccg gacctacccg gctaggcctt cccgccggtc atgaggtcga agacccgctc
    25861 caggtcgtcc agggagaagt actcgatcgt gatggagccc ttcctggcat cccccttgac
    25921 ctggaccttg gtccccagga acccgatgag cttctgctcc aggtcccgga ggtcgggatc
    25981 caggggtctg cggggcgcct ccggcttgcg ggccggggcg gggcgcttgc ccgcgttgag
    26041 gtccgcggcc agggcttccg cctcccggac ggagagcccc tcggccagga tacgccggaa
    26101 gaggtcccgg cggtcctcgt cggagctcac cgccaggacg gcccgggcat gcccgggact
    26161 cagatccccg gagcgcaggg cctccagcat ctccgcgggt agcttgagga ggcgcagggc
    26221 gttggccacg gtcgggcggc tcttcccgac cttctcggcc acctcctcct gggagagcgc
    26281 ggccgcctcc atgaggacct tgt
//
LOCUS       DAOTEI010000030        26292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1211_length_26292_cov_4.880855, whole genome shotgun sequence.
ACCESSION   DAOTEI010000030 DAOTEI010000000
VERSION     DAOTEI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 26292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26292
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1211_length_26292_cov_4.880855"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>316
                     /locus_tag="P5313_04650"
     CDS             <1..>316
                     /locus_tag="P5313_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY79686.1"
                     /translation="PLALAVRKARPDWSRLLVEKGAAVFARNSTGLTPLAAAVSIGPE
                     AVDPLLIPSTIRARDDSGNGLLHAAVLLKAIPETLVLMMERGVDPSTRNNAGDTPLHI
                     AVR"
     assembly_gap    317..326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <327..1615
                     /locus_tag="P5313_04655"
     CDS             <327..1615
                     /locus_tag="P5313_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY79687.1"
                     /translation="RVLLAARTDIFAANVRGETPLGLALASPSNPKRWFFTEPVLAAR
                     DGAGNTPLHYAASEGLAQAADFLVSVGASPEARNADGQTPLHSAVRKDSPDSIRALVT
                     AGADLSARDLTGATPLHQAVYWNARRSMESLVLAGSDVNARDFAGASPVFEAVRRQDA
                     SAVRWLLDRGADPAARNDAGRTPLHDAAVNGDLGTVQLLLAAGAAPNARGDGGATALH
                     EAVGSDRVNVIPALIARGADIHARNFAGETPLTLALGRGTEVLKALLTGGAALSVDSD
                     GRSVLRVILEAKPTAGFVEIALAAGARTGDRDRSGATALHAAVSQGYTEIVRLLVVGG
                     ADVFARDRDGNSPLSLAMAAGQEALGAVVTASNVNARDLLGNRPLHYAAQTGNAAAAE
                     FLLNLGADRGARNSAGDTAAEAAEKRGFMELAGKLR"
     gene            complement(1755..2771)
                     /locus_tag="P5313_04660"
     CDS             complement(1755..2771)
                     /locus_tag="P5313_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY79688.1"
                     /translation="MSESAFPMRTAVKKKKLVPAFILKKHKKPKHPKETDPLALYLKQ
                     ISRYPLLTAEEELELGERIVRSRASVRRIDEEAALGKLPMKRQAERAESDRELVHCKN
                     RMISSNLRLVVSIAKNYQHRGLSLLDLIDEGNIGLIEAVERFDYTRGCRFSTYGTWWI
                     RQAIIKSLADKGRVIRIPIHMLNTIKKCYFVAKQLTQELGRDPNPEELAEKLGMDSRK
                     VKEIMKLSQETASLDTTVDEDNVTHLSDLIKDETAVEPFEEVFSMTLQDTLGSVLKNL
                     NQREMTIITLRYGLNGEGPRTLEETGKILGITRERVRQIQEKAILKLKELQQLSEYQE
                     SGGR"
     gene            complement(2935..3786)
                     /locus_tag="P5313_04665"
     CDS             complement(2935..3786)
                     /locus_tag="P5313_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY79689.1"
                     /translation="MRLNRFLFSALLFSIAAAALCPESLVHVVEKGQTIYGIARTYGV
                     PFESILAANGIEDPRRILPGTRLVIPSSHKVAKGETLYGIARAYGIPLEDILKVNKLA
                     PNSLIKPGDLLVIPGAAVRVPPPAPGALAPAPASGAGGTTAPPGTKPDPAAPVEPAAK
                     PPAKPPTGGAWPAPGEAVYLDGKLFGVGIRTEENIPIRAVRSGTVISAGPFRGYGRVA
                     FVQAKDGLVYVYGGAQNLSVRIGESVRSGTEIGRAGIDLKEGRPIVYFLVFKQGNPLD
                     PAKVPRE"
     gene            complement(3822..4643)
                     /locus_tag="P5313_04670"
     CDS             complement(3822..4643)
                     /locus_tag="P5313_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79690.1"
                     /translation="MKFKTIFYLFNAVVAFSFLFIFLFPILILGGEYAAGFWKTNWLL
                     GAAFLAVLGILNGFFLSNWKLFQLVEREDWSALSSWLEERIFRKGSLRGQYVRLYVNS
                     AMLRSDLEAIDRLEALLREKKPALLRRLAVLFGVGYLLRNEADSSLAFFEPFLESPEV
                     EARGWLWLDYSFSLVLRNRAPEALPWLRKASGQKDPVLSLLAAYLTANLAAPALPAGP
                     DRDAARSTAEGFRDRLRKRFDAERWSREVEKAKNDVHVVILSRLIDDAGRWLFQG"
     gene            4768..5934
                     /locus_tag="P5313_04675"
     CDS             4768..5934
                     /locus_tag="P5313_04675"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRY79691.1"
                     /translation="MNEIPVKDLEPDTYFSKPVLLDQDYILLSTETPVTRTLINHLIE
                     WGYRKVFTEGVPQQIIPDADSEGAAASENGNPVVAVNAVDSERLEHVRGFYEQFATFV
                     ESVYDRYVTKSELVLAEVSVRIKSLCEVIAENRRFILRVQSLVQPHSNYLVSHSTRTT
                     VLSIVIGSALRLPNHRLIELGTAALLHEIGMVRLPPQLYMASRQLSPQERRSITAHPI
                     LGYNVLKDKQVPLAISLAALEHHERMNGTGYPRGIAGDKISLYSRIIAVACSFDAVTA
                     SRPFKEARDGYSGMVDLLKNEGKKYDETVIRALVYSLSIYPIGSYVLLTNGKPAQVVD
                     VNPENPRFPVVQIVGMRTPDGKDLIARTAETGVRILRPMTPEEIKDLTKEPASS"
     gene            6077..7726
                     /locus_tag="P5313_04680"
     CDS             6077..7726
                     /locus_tag="P5313_04680"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRY79692.1"
                     /translation="MDESPLQTIRYRYEPKLPAILRSGVDRVAVSLGEPSRAADGIRD
                     LFPKTYGKPVARFVEGANPDMRRKLTVGVILSGGQAPGGHNVIAGLFDGLKKGNPESR
                     LVGFAGGPGGLVNDKAVEITADLMDRYRNTGGFDIIGSGRDKIETPEQFEKAAAAVRS
                     RGLDAVVVVGGDDSNTNAALLAEFFAGRGESVSVIGVPKTIDGDLKNEYIETSFGFDT
                     AVKTYSELIGNIERDAASARKYWHFIKLMGRSASHIALECALQCRPNVALISEEVEAR
                     GWTLARITDYICDAVAARAEKGENFGVVLVPEGLVEFIPEFKALISRLNDLLAAQAQG
                     FAALSEPAARVEFVASRLPGELAALYRGLPGDIKLQLIMDRDPHGNVQVSRIETEKLL
                     IALVEARLAERKAAGTYKGKFSWQNHFFGYEGRCAFPSNFDADYCYSLGFTAFALIAS
                     GLSGYISSVRNLTAKAEDWTAGGAPLTMMMNLERRHGSQKPVIRKALVDLEGRPFQAF
                     AAGREDWAVRTAYRFPGAIQYFGPPEVCDAVTRTLELERGK"
     gene            complement(7750..9717)
                     /locus_tag="P5313_04685"
     CDS             complement(7750..9717)
                     /locus_tag="P5313_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRY79693.1"
                     /translation="MVLFIAAAVLASCSRAGDSGQAAPAAPEPGSPASAVSEVPAPRF
                     PEGGQAELVRGDTGARILRGEERLPAEPGTLLALGDRVRTGASDPAELRLGGLAAVLL
                     LPDTEVELRAARLAPGAAHVEVFVSSGAALFDVRSLSAGESFLVAAPRILSGVRGTRF
                     LVRAGEASVTAVREGRVAVLPSGPVLDRLAAAARTDGVARAALRTLVALAPVAEPGRE
                     IRVDDPALARAEAAYAALESALDGLAPLPLPPGFPEEPWFAASPPELPAPDPRARDSL
                     ARARESAAAAAPALAVPVAAGPETLRGFDRFRDFRSGRLSPPPAASGPATPSPHPAEL
                     GRTGISPDPLAGSLARVPDAGILLAADGKGVLYAFDPEGRVRWSLRTANRGDPRGYPV
                     SFKGTAYYAGNAELVAVDGATGAVVARRGTEPGLDPGTRPSPFPDSLLLPTQAGIEVL
                     DPRTLERRTSLPLAAGPGTLPVQRDSFALVVDRQGTLLLVDPVSGTVRASTPTGARGY
                     SAVSPRILEEKACFADPTGLVVMVDLERMAVLWERRAGVPVLTDLELAREGVLAYGNG
                     TLFGYRLDGEVLMAPVSGVSAPPLLARGSVYYGTEKGELVAAQADPWRIRGSVPLGDV
                     PSARPLLVGDTLYVGTRGGKLVRIDVTQLPR"
     gene            10030..12516
                     /locus_tag="P5313_04690"
     CDS             10030..12516
                     /locus_tag="P5313_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY79694.1"
                     /translation="MFEFLKRMFRKAPEREPGLIPEELWTSELDQPASGRFLPVSEDR
                     YRASYRKDGGFELTLLRKNLFAWAEVPEPRHEDLALEAQIEFTGGEGLGAAGFLLRAE
                     GDRDFLYVLVSNRGFLRVDAVFNGKPHPVVPWTECPEPVGPSFNLKIVLSGSLLVAAV
                     DDLWTAECEDETVRQGRLAFAAQNYDEAGEASCVLRFILTETRPMEVEARRLRWSVLE
                     KADPGQRVRLARSLAAGGLDVPALVQLRKAEKSRPLDADGLFLRAECSLRLGLLEDAE
                     TALRACLAASGDDPGERARLAREELANLLYLRGNYLELRDELAGRDLSASPRLAGLLG
                     HAYWNLGNWGDAADCYGRAARAEPDMPIFALNEARSWDQAGRREEAAEAYERAARGFL
                     AQESPEDLEECLGRLKALRPRSDATAALAAKMLFRDGKKAEAEKIFRKLAAKGTEDSA
                     VLYLLGLILSERGERAEAVGLLRRACALESGFPLYWFRLAEALFLSGDAEGAAEPLSR
                     ARDLAPGDGWILNLGGLLAERRGDSGEAIRCFRAARETLPDQVEPAVNLSEALDAAGS
                     TAEALEILSGFPDSPAARNQAGNILARRGRLEEAAAEYDRAVRAAAGTASPEIEAEYR
                     ANYAACCLELDRYSDAEDQLRRALQRSPSRRVYLLTGDLAARYGDRYRAETAWMAGLE
                     LFPGDPDLLERLFRHRLYRGALGEAEQTARELERSDPARGERARAELRDATTVRYECA
                     SCGREWRAPKDLPARAARTIRAQPPDDSPAGACPDCGKVYCVGCRKDHLEDLRFTCPG
                     CGTFLKLSDDRLRHLVLESLGGKRKGSRGA"
     gene            12625..13302
                     /locus_tag="P5313_04695"
     CDS             12625..13302
                     /locus_tag="P5313_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HRY79695.1"
                     /translation="MKRSILSLSIALLLAAATPAQDIVTAEQYFAGVSERYAQITDYE
                     ARISIRTQRTTMLGTVWYKAPRLLRIDFTQPADQVICFNGENLVVYVPEYRAVLSQSV
                     STSGSGGAGLATADGLRTLRRNYTVAYEQTPNPVPLEEGSPELVVKLLLNRRTVAEGF
                     RTIVLSVNPDTRMIRRISGITVSNETFVFDFSGIRTNQGIPETRFVYDSPASANVYNN
                     FLFKTEN"
     gene            13320..13511
                     /locus_tag="P5313_04700"
                     /pseudo
     CDS             13320..13511
                     /locus_tag="P5313_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            14502..15815
                     /gene="rimO"
                     /locus_tag="P5313_04705"
     CDS             14502..15815
                     /gene="rimO"
                     /locus_tag="P5313_04705"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRY79696.1"
                     /translation="MDLHGCAKNQVDAEEIRARLEAEGWTARESPEGAGILVVNTCGF
                     IESAKKESLDAVLDLKARYPGSRVLLAGCLAQRYSEALCADLPEADGIFGNGDLSRVT
                     EAAEALRRGGRPVLVPGTAPPARAPRDTLFSYPRSAYLKISEGCSNRCSYCAIPLIRG
                     DARSRDVADVAGEARDLVSRGIFEINLIGQDLGAYGLDSIGRQALPDLLTELRRIDAD
                     FRVRMLYIHPDHFPGAILDHCAADPRILPYFDLPFQHASRSVLSAMNRRGDAPTYLRL
                     IDGIRERLSDAVLRSTFLVGFPGETEEDFAVLRDFQDRARLTWLGAFEYSREEDTPAA
                     ALKGRVPRKTASERRRLVEEAQRPITEADLRRFLGRDLEVLVEEPVEGEELSLGRSYA
                     QAPEVDGLVVLNARLDPGRVVRARVVAVNGVDLEAALYIPPSPAP"
     gene            15824..17935
                     /locus_tag="P5313_04710"
     CDS             15824..17935
                     /locus_tag="P5313_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY79697.1"
                     /translation="MPELPEYLQDLNPEQLAAVRHEGGNLLILAGAGSGKTRVITTKI
                     AWLVRERGVAPESVLAVTFTNKAAREMRDRASAIDPECARAYLRTFHSFGAWFLRRNA
                     AAAGLDPNFTIYDDDDSTTLLHGTAPDLTRAECGRLASRIARAKDYGLAPDAPDLEAR
                     FPDRNFRRLYAGYEKRLRETGNVDFGDLILLPARVLRDDEAIRNRFHQRFRVILVDEY
                     QDSNVAQFELLKVLAGGGAYVCVVGDDDQSIYRFRGAEVRNILEFPEVFPGTEIVRLE
                     RNYRSFQSILDVAGAVVARNEGRLGKTLKAVKAGGPKAKLALLADQDEEAAYASRVAE
                     ARASRTGRWSDTAVLYRTNAQSLAFEHLFNKRGIPYRIVGSVRFYEREEVKDALAYLA
                     VLSNPRDEVNFRRVVNKPSRGVGDTSVQAILEAAYASDGDLLSASRGAGPLLKTAKSR
                     SGLSAFVSLVDGLRGLLDAPGEGRPLSGLVERTVKDSGLAEYHRDQDEIAGTQRAANL
                     DELVNAAADFELSPAGLAEFLETIELDRRLSDQEESLEAVTLITMHNTKGLEFPVVVV
                     TGLEQGLFPRDDDEGEDLEEQRRLFYVAATRAKEELHLCSCRSRLLRGRRADFLPSRF
                     IHEIPPGLYETLGGRLPAPAPEGPWKPGLPVYHDDHGSGYVIQVKAGPPDSGPLVFVK
                     FETGRVLQFFPKFTRKLEALK"
     gene            17932..18834
                     /locus_tag="P5313_04715"
     CDS             17932..18834
                     /locus_tag="P5313_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79698.1"
                     /translation="MKTDLPPVRRMRLWRLYAEDGRRFLDLRLLDNRGFLGAKGRFAG
                     TRAKNGIDLGLLKEAPGRHAERLRKALAAEFPRLAAFRFFRNEERALATLSRTRSGLY
                     DPLARHAEAEDRPAAGAGRSPAAGMAAAEGAPALAVHRFFLAEEDRARLEAFPALLIP
                     LPCPEPFGPAVLGFSEAEAAASVPEDELPPIMPFTAMRAWEDFRGMSVRLTGGRRARS
                     GEGTPAYAEDLWKRSDRRLTPFFERRGPYLYARCARADWEGFRARALEAGCVLTPEWE
                     LPSIVPGDFDDGELKNLAKALEIL"
     gene            complement(18831..19766)
                     /locus_tag="P5313_04720"
     CDS             complement(18831..19766)
                     /locus_tag="P5313_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY79699.1"
                     /translation="MRNTTKTAGGAPGTEAAAVPVHPSRGAGTGVLLALLSAASFSTL
                     GIFAKLIYAEGFSTSQALAWRFTGAAAFLWIWILAADRRLPPLRPNLPVLALGLLGFA
                     PQAGLYFVTLRFLDAGITSLLLYLYPSFVVLLSAVFLGRRPTGVQLAALALSLTGCVT
                     TFFRAGRYPAIGIGFGILVALTYAAYLVVGERVLEGRKPVHSTAFIMTAAAAAYWTLT
                     ALTGGFKAPEALSSVLGLAGIAVAATVIPIVTLFGAMQRIGASNTSLVSTVEPLIAVL
                     LSALILGERFGLQQIAGGVLIVGAVVLIQAAARRA"
     gene            complement(19798..21312)
                     /locus_tag="P5313_04725"
     CDS             complement(19798..21312)
                     /locus_tag="P5313_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79700.1"
                     /translation="MAKETKVYDYKTAAVRDRLASEFRNTRGEATVADLVVRTGLPKY
                     QVETEVRAVADEFGARLRVTESGEILYSFPRGLHSRYRGLGPTLKRLWKALRKGAAAV
                     AAFLFKIWIVVMLVGYFVLFLALTLAALAASIAAQTAGNRDSRGRNRGGGGGLMFTGR
                     ILELFVRIWFYGELFKSPEQRYHERERRLARSADRRPLHKAVFSFVFGDGDPNEPWAE
                     TEKKAVTAFIQANRGLISLEEFMTLTGHRPGEAEDAINRYLYEFEGSPEVTPSGTIYY
                     FFPALMRRKDERDRTFGGSAPLKRLVPFSSNPRKANVWFAALNGVNLAFGSFFLVNAL
                     SRGIPRLPPGAERIPADFYLIVYALISQVAADPLSAILWGLGFVPAAFAVLFYLVPFL
                     RSLRLARTNEEIKRQNLRKLVYARVWDEPGRVSPGAAAVPDEAANPADRKAAEKAVEE
                     LAAWAGGEPDAGGAWSFPELARRKADVEAARRAVKPEDYALGGAVFDSEGPGAG"
     gene            complement(21330..22802)
                     /gene="glgA"
                     /locus_tag="P5313_04730"
     CDS             complement(21330..22802)
                     /gene="glgA"
                     /locus_tag="P5313_04730"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRY79701.1"
                     /translation="MKILMVSSEATPFAKSGGLADMVGALTRALDRAGHDVRIVLPRY
                     YCVPKTTLKKLEGPLCVPVGWGEEWCAVYESTLPGCKAPVYFLDHERFFGRDGIYGTK
                     EETDFRDNPQRFSFFSRAAFQLCRKLDWIPDVLHAHDWPAGLVPVYRRYVESASEFAR
                     TATVFSIHNLGYQGIYSKEIFPYFRLPWDLFHKAGLEYYDAINLLKGALASSDQLSTV
                     SPTYAREIQTPEFGCSLDGLLRSRSSDLIGILNGVDFDDWDPSSDRHLPAHYSAADLS
                     GKAACKAALQREFGLPEDPDVPLIGMVTRLTDQKGIGELFGPLYGSAYRICTDMILQV
                     VLLGSGDRWCEDEVQALAARIPNFRAKIGYSDRTAHLIEAGSDFFLMPSRYEPCGLNQ
                     MYSLRYGTLPIVRRTGGLADTVENYRQDTGEGTGFLFDLLTPRSIYDTVGWAVWAYYN
                     KKDHIAAMRKRAMTREFTWDRAAGEYGELYARALRRVRVD"
     gene            complement(23213..26215)
                     /locus_tag="P5313_04735"
     CDS             complement(23213..26215)
                     /locus_tag="P5313_04735"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRY79702.1"
                     /translation="MANGRTLNNTGGTLQVVSSAGAVSIVSPGTMTLSGNQLDLNALT
                     LHVSGFSTAVAHTLDPSDILILDGTATFSGGLTLDGAGALVDVGVQTLNVSGGTGFTL
                     TDGDAQVTTGTINATSGLTITNGSVTATTGRINTGASALNLAAGGSIAFTGAGAPSLS
                     AGPVTNAGIITLAGGTASTGSVTNTGTITFDTAAGATWTAAGFVTSSGAINNTFSNTI
                     STNGNVTISGTFATPLNSTLRMTGSPTNLNATIPLGNFIANAGGTITIAANLGLANDL
                     TIQTGTLASGNFTITIAGNWTNATGVITAFTPGTGIVEFTDSALATVSGPNQWYNLRY
                     NQPGGRIRFQRSNIQRIVAGGTFQAIGTIANHVTITRDDLGDDGADLNWLTGVPPNPV
                     LMWQLDKTGTGNLDFQYVDVWYSDARSNPIAYNSLYVTLRTVVPDGAPPLEQGLTCYA
                     WFSGMTVVYSYTEDSDDDGRIDRIRVTAQGALNMDFSDLEITVTGYEIDTTVYPSGFS
                     GTVGDADFYIHLVEKPYLDSSRTPSWTMTNNTALKSNAAFPNDYTLVLNLADPMIPAD
                     TVPPRIAYTLAVPSTPDVFFAFNERVERAGGGVLTAAEIPGSVSVSLPGLIGDGDVYG
                     VLAAYGGNFSIANIVGELARTVNAGITDQAVPLYDWSTDPVASAYITTVPDYPAGSLP
                     NPISLTSNTHRVSDLLVSVPPSDTVGAWSYSGGANADSFFVWPLWAKDEAYVPGVPDV
                     DYPTIPELDASGQYVGFVRLFDGSRFLRDQDFDLQVEDALGLGSAPQILYKSDVSAAY
                     LSAVPGIWLPTYAEPDFSGLASYPFTGFVSNLTGTGTTPIWDYSFLASDPEIYDRARF
                     EFYFRLPGPYPADLYAGRLDMDPDATAIPADWYRRVRPFGILIRNVVTQKGGVSILNN
                     VIDPTTGERTRLHYTIGQAGPVTITVFTLDGDVVRSLQRGTMTPGDYTASWDGRNRAG
                     DPVARGMYFIRIVAPGIDEIRKVMVVRY"
BASE COUNT         4367 a         8947 c         8943 g         4025 t
ORIGIN      
        1 cccccctggc cctggcggtc cgcaaggcgc ggcccgactg gagccgtctg ctcgtggaga
       61 agggagccgc cgtcttcgcg cgcaacagca ccggactcac ccccctggcc gcagcggttt
      121 ccatcggccc cgaggctgtg gaccccctcc tcatcccctc cacgatccgg gcccgggacg
      181 actcgggcaa cggactgctg cacgcggcgg tcctcctgaa agccatcccc gagaccctgg
      241 tcctgatgat ggagcggggg gtcgatccct ccacccggaa caacgccggg gacactcccc
      301 tccatatcgc ggtacgnnnn nnnnnncccg ggtcctcctg gccgcccgga cggacatctt
      361 cgcggccaac gttcggggcg agacccccct gggcctggcc cttgcgtcgc cgtccaatcc
      421 caagcggtgg ttcttcacgg agccggtcct ggcggcccgg gacggggcgg gcaacacgcc
      481 ccttcactac gcagcttcgg aaggactggc ccaggccgcg gacttcctgg tctccgtcgg
      541 agcctccccg gaggcccgga acgcggacgg acagaccccc ctccactcgg cggtccgcaa
      601 ggactccccg gacagcatcc gcgccctcgt gacggcgggc gcggacctct cggcccggga
      661 cctgacggga gcgactcccc tgcaccaggc ggtgtactgg aacgcccgcc ggagcatgga
      721 atccctggtc ctggccggga gcgacgtcaa cgcccgggat ttcgccgggg cttccccggt
      781 gttcgaggcg gtgcgccggc aggacgcctc cgcggtgcgc tggctcctgg accggggcgc
      841 ggaccccgcg gcccgcaacg acgccggccg gactccgctc cacgacgccg cggtgaacgg
      901 agacctggga acggtacagc tactcctggc cgccggcgcg gccccgaacg cccgcggaga
      961 cggcggagca acggccctgc acgaggcggt aggctcggat cgggtgaacg tcatccccgc
     1021 cctgatcgcc cggggggcgg acatccacgc ccgtaacttc gccggggaga cccccctgac
     1081 cctggccttg ggccggggca ccgaggtcct caaggccctg ctgaccgggg gcgccgccct
     1141 ctccgtggac tccgacggcc gttccgtgct ccgggtgatc ctggaggcca agcctaccgc
     1201 gggcttcgtg gagatcgccc tggccgcggg ggcgcggacg ggggaccggg atcggtccgg
     1261 cgcgaccgcc ctgcatgccg cggtctccca gggatacacg gagatcgtgc gcctgctagt
     1321 ggtgggcggg gcggatgtct tcgcccggga ccgggacggg aactcccccc tgtccctggc
     1381 catggccgcg ggacaagagg ccctgggagc ggtggtcacc gcctccaacg tgaacgcccg
     1441 ggacctgctc gggaaccgtc ccctgcacta cgcggcccag acgggaaacg cggcggcggc
     1501 ggaattcctg ctcaacctgg gagcggaccg gggagcccgg aactccgccg gggataccgc
     1561 tgcggaggcg gcggagaagc ggggcttcat ggagttggcg gggaaattga ggtgacagga
     1621 ctcagtcgat aggagccagg taacaggact caggcgactg gagcagttcc agtcggccgc
     1681 ggactacctc caggcatctg tcatctggag gtccctctga gtcctgagtc cggagtcctg
     1741 agtcccggct cccgtcacct gccgcctgac tcctgatact ccgacagctg ttgcagctct
     1801 ttgagcttca ggatcgcctt ttcctgaatc tggcggaccc gctcccgggt gattcccagg
     1861 atcttgccgg tctcctccag ggtccggggg ccttccccgt tcagaccgta gcggagggtg
     1921 atgatggtca tctcccgctg gttcaggttc ttcagcacgc tccccagggt gtcctggaga
     1981 gtcatggaga agacctcctc gaagggctcc accgcggtct cgtccttgat caagtcggac
     2041 aggtgagtga cgttgtcctc gtccaccgtg gtgtccaggg aggcggtctc ctgggagagc
     2101 ttcatgatct ccttgacctt ccgggagtcc atgcccagct tctccgccag ttcctcgggg
     2161 ttcggatccc ggccgagctc ctgggtgagc tgtttggcca cgaaatagca tttcttgatg
     2221 gtgttgagca tgtggatggg aatgcggatg acccgcccct tgtccgccag gctcttgatg
     2281 atggcctgcc ggatccacca ggtgccgtag gtcgagaagc ggcagccgcg ggtgtagtcg
     2341 aagcgctcca ccgcctcgat caggccgatg ttcccctcgt cgatcaggtc cagcagggaa
     2401 agaccccggt gctggtagtt cttggctatg gacacgacca ggcgaaggtt cgaggagatc
     2461 atccgattct tacagtgcac cagttcccga tccgattccg cgcgctccgc ctggcgtttc
     2521 atcgggagct tgcccagggc ggcctcctcg tcgatcctcc ggaccgaggc ccgggatcgg
     2581 acgatgcgtt ccccgagctc cagttcctcc tccgcggtca ggaggggata acgggagatc
     2641 tgcttgaggt agagggccag gggatccgtt tccttgggat gcttcggctt cttgtgcttc
     2701 ttcaggatga aggcgggaac cagttttttc ttcttgacgg cggttcgcat cgggaaggcg
     2761 ctctcactca tgttcgctcc cttcctggta tccaccccga agaacggccg aattggctgg
     2821 gtcctccgga gtgtgattga atatatcgca gttgacaaaa aaaacaagca ttattttact
     2881 catattttac aaaatatcaa tttttattcc tgatatttta ggtacccgtc cggatcactc
     2941 ccggggaact ttggcggggt ccagggggtt cccctgcttg aagaccagga agtagacgat
     3001 gggccgcccc tccttcaggt cgatacccgc gcgtccgatc tcggtgccgg atcggacgga
     3061 ttccccgatc cgcacggaaa ggttctgggc cccgccgtag acgtagacca ggccgtcctt
     3121 ggcctgcacg aaggccaccc ttccgtagcc ccggaaggga cccgcggaga tcaccgttcc
     3181 ggagcgcacc gcccggatcg ggatgttttc ctccgtccgg atgcccaccc cgaagagctt
     3241 cccgtccagg tagaccgcct ctcccggggc gggccaggcc ccgccggtcg gaggctttgc
     3301 gggaggcttg gccgcgggtt cgacgggcgc agccggatcg ggcttcgtgc cggggggcgc
     3361 agtcgtgccg cctgctccgg atgccggagc cggggcgagg gccccggggg ccggaggggg
     3421 aactcggacg gcggcgccgg ggatcaccag caggtctccg ggcttgataa gggagttcgg
     3481 ggccagcttg ttgaccttga ggatgtcttc caggggaatc ccgtaggccc gggctatacc
     3541 gtagagggtc tctcccttgg ccaccttgtg ggaggagggg atcaccaaac gggtgccggg
     3601 caggatcctg cgcggatcct cgatcccgtt ggccgccagg atggactcga agggaacccc
     3661 gtaggtccgg gctatgccgt agatcgtctg gcccttttcc acgacgtgca ccagggactc
     3721 cggacacagg gccgccgcgg cgatggagaa cagcagggcg gagaacagga atcggttgag
     3781 tctcatccga cttctattct cggccgggga cggaggaatc cttacccttg gaagagccac
     3841 ctgccggcgt cgtcgatcag gcgggacagg atgacgacgt gcacgtcgtt cttggccttc
     3901 tcgacctccc ggctccagcg ttcggcgtcg aaacgcttcc ggagccggtc ccggaagcct
     3961 tccgcggtcg accgggccgc gtcgcggtcc ggccccgcgg gaagggccgg agcggcaagg
     4021 ttggcggtca gatacgcggc caggagggaa aggacgggat ccttctgccc ggatgccttc
     4081 cggagccagg gcagggcctc gggggcccgg ttccggagga ccaggctgaa ggaataatcc
     4141 agccagagcc atccccgggc ctcgacctcg ggggactcca ggaagggttc gaaaaaggcc
     4201 agggaggaat ccgcctcgtt tcgtaggagg tagccgaccc cgaagagtac cgccagccgg
     4261 cgcaggaggg ccggcttctt ttcccggagg agggcttcca accggtcgat ggcttccagg
     4321 tcggagcgga gcatggcaga gttcacgtac aggcgcacgt actgaccgcg aagggaaccc
     4381 ttccggaaga tccgctcctc cagccaggag ctcagggcgg accaatcctc gcgctccacc
     4441 aactggaaga gcttccagtt ggacaggaag aagccgttca ggatccccag gacggccagg
     4501 aacgccgccc ctaaaagcca gttggtcttc cagaatcccg ctgcgtactc cccgcccagg
     4561 atcaggatgg gaaagaggaa tatgaacagg aaggagaacg cgacgacggc gttgaaaaga
     4621 taaaaaattg tcttaaactt catccgggaa cgctccgaaa ggtatgcggg atgcgctgat
     4681 cccggcagat gggcgatacg ggaaacagcc ttgccggaag cctcgctggt agtctagtat
     4741 gaaggtttcg gggggtcaat ggggaccatg aacgagatac ccgtcaagga cctggaaccg
     4801 gatacctatt tctcgaagcc cgtcctcttg gatcaggatt acatactcct ctccacggaa
     4861 acccccgtaa cccggacctt gatcaatcac ctgatcgaat gggggtacag gaaggtcttc
     4921 accgagggcg ttccccagca gatcattccg gacgcggact cggagggggc cgccgcctcg
     4981 gagaacggga atcccgtcgt ggcggtgaac gccgtggaca gcgagcggct ggagcacgtc
     5041 cgggggttct acgagcagtt cgccaccttc gtggagtccg tctacgaccg gtacgtcacc
     5101 aagagcgagc tggtgctggc ggaggtttcc gtccgcatca aatccctctg cgaggtcatc
     5161 gcggagaatc gcaggttcat cctgagggtc cagtccctgg tccaaccgca ttcaaactac
     5221 ctggtgtccc actccacccg caccacggtg ctctccatcg tgatcgggtc cgcgctccga
     5281 ctgccgaacc accggctcat cgaactgggc accgcggccc tcctgcacga gatcgggatg
     5341 gtgcgcctgc cgccccagct ctacatggcc agccgccagc tcagccccca ggagcgcagg
     5401 tcgatcaccg cccatcccat cctgggctac aacgtgctca aggacaagca ggtccccctg
     5461 gccatcagcc tggcggccct ggagcaccac gaacggatga acggcaccgg ctatccccgg
     5521 ggcatcgcgg gggacaagat ctccctttac tcccggatca tagccgtggc ctgttccttc
     5581 gacgcggtga ccgccagccg tcccttcaag gaagcccggg acgggtactc cgggatggtg
     5641 gacctgctca agaacgaggg caagaaatac gacgagaccg tgatccgggc cctggtatac
     5701 tccctgtcga tctatcccat cggctcctac gtcctcctga cgaacggcaa gcccgcccag
     5761 gtggtggacg tgaacccgga gaatccccgc ttcccggtcg tccaaatcgt aggcatgcgg
     5821 acccccgacg ggaaggacct catcgcccgc accgcggaga ccggggtgcg catcctccgg
     5881 cccatgaccc cggaggagat caaggacctg accaaggagc ccgcgtcctc ctaagctaag
     5941 caggttgttg aaatacctcg agcatttcaa cgacctgcta gacttcccta atccttcccc
     6001 cttgcgctac actcagggcg aacattcggc cgggtcggca ggtccgcccg gcggacgccg
     6061 aaacaaggag ccgaacatgg atgaatcccc cctccagacc atccgctacc gctacgagcc
     6121 gaagcttccc gccatcctcc gcagcggcgt cgaccgcgtc gccgtatccc tgggcgaacc
     6181 ctcccgggcc gccgacggga tacgggacct gttcccgaaa acctacggaa aacccgtggc
     6241 ccgcttcgtc gagggagcaa atccggacat gcgcaggaag ctgacggtgg gagtcatcct
     6301 ctccggaggg caggccccgg gagggcacaa cgtgatcgcc ggcctcttcg acggcctcaa
     6361 gaagggaaac ccggaatcgc gccttgtcgg gttcgccggg ggccccgggg ggctcgtgaa
     6421 cgacaaggcc gtggagatca ccgcggacct catggaccgg taccggaaca ccggaggctt
     6481 cgacatcatc ggttccgggc gcgacaagat cgagaccccc gagcagttcg agaaggccgc
     6541 cgcggccgtg cggtcccggg gcctggacgc ggtcgtcgtg gtgggcgggg acgattccaa
     6601 cacgaacgcg gccctcctgg ccgagttctt cgcgggccgg ggcgagagcg tgtccgtcat
     6661 cggggtgccc aagaccatcg acggggactt gaagaacgag tacatcgaga ccagcttcgg
     6721 gttcgatacc gcggtcaaga cctactccga actgatcggg aacatcgagc gggacgccgc
     6781 cagcgcccgc aaatactggc acttcatcaa gctgatggga cggtccgcca gccacatcgc
     6841 cctggagtgc gccctccagt gccggcccaa cgtcgccctg atctccgagg aggtcgaggc
     6901 ccggggctgg accctggccc ggatcaccga ctatatctgc gacgccgtgg ccgcccgtgc
     6961 ggagaagggg gagaacttcg gagtggtcct ggtgcccgag ggcctggtgg agttcatccc
     7021 ggagttcaag gccctgatct cccggctgaa cgacctcctg gcggcccagg cccaggggtt
     7081 cgcagccctc tccgagcccg ccgcccgggt cgagttcgtg gcatcccgcc tgcccgggga
     7141 actcgccgcc ctctaccgcg gcttgcccgg ggacatcaag ctccagctga tcatggaccg
     7201 ggacccccac ggaaacgtac aggtctcccg gatcgagacg gagaagctcc tgatcgccct
     7261 ggtcgaggca cgcctggccg aacggaaggc ggcggggacc tacaagggca agttcagctg
     7321 gcaaaaccac ttcttcggct acgaggggcg ctgcgccttc ccctcgaact tcgacgcgga
     7381 ctattgctac tccctgggtt tcacggcctt cgccctgatc gcctcgggcc tctcgggcta
     7441 catctcgtcg gtgcggaacc tcaccgccaa ggccgaggac tggacggccg ggggggcccc
     7501 cctcaccatg atgatgaacc tggaacgccg ccacggctcg cagaagccgg tcatccggaa
     7561 ggccctggtg gacctggagg gcagaccctt ccaggccttc gccgccggcc gggaggactg
     7621 ggcggtccgc acggcctacc gcttccccgg ggcgatccag tacttcgggc cgccggaggt
     7681 ctgcgacgcg gtcacccgga ctctggagct ggagcggggg aagtaggcgc cccggcgccc
     7741 gacatactcc tagcggggaa gctgggtcac gtcgatccgg accagctttc cgccccgggt
     7801 gcccacgtag agggtatccc ctaccaggag ggggcgggcg ctgggcacgt cccccagggg
     7861 gaccgagccc cggatcctcc agggatcggc ttgggccgcc acgagctccc ccttctcggt
     7921 cccgtagtac accgagcccc gggccaggag gggcggggcg ctcacccccg acacgggggc
     7981 catgaggacc tccccgtcca ggcggtaccc gaacagggtg ccgttcccgt acgccaggac
     8041 cccttcccgg gcgagttcca gatccgtgag caccggcacc cccgcgcggc gctcccagag
     8101 cacggccatc ctctccaggt ccaccatcac caccagcccc gtgggatcgg cgaagcaggc
     8161 cttctcctcg aggatccggg gggataccgc cgaatagccc cgggcccccg tgggcgtgct
     8221 ggcccggacc gtgccggata cgggatccac caggaggagg gtcccctgcc gatccaccac
     8281 cagggcgaag ctgtccctct ggaccgggag ggtgcccggc ccggcggcca ggggtagcga
     8341 ggtccgccgt tccagggtcc ggggatccag cacctcgatg cccgcctgag tcggcaggag
     8401 gagggaatcc gggaagggcg aggggcgggt tccgggatcc aggcccggtt ccgtcccgcg
     8461 acgggctacc accgccccgg tcgccccgtc cacggccaca agttcggcgt tacccgcgta
     8521 gtacgccgtg cccttgaagg agaccggata cccgcgggga tccccccggt tggcggtccg
     8581 gagactccag cggacccttc cttccggatc gaaggcgtac aggacgccct tcccgtccgc
     8641 ggccaggagt attcccgcgt cgggaacccg tgccagggaa cccgccaggg ggtccgggga
     8701 tatgcccgtc ctcccgagct ccgcgggatg gggggaaggg gtcgccgggc cggacgccgc
     8761 tggcggtggg gaaagacgcc cggagcggaa gtcccggaac cggtcgaacc ctcgcagggt
     8821 ttccggcccc gcggctacgg gcaccgccag ggccggcgcg gcggccgcgg cggattcccg
     8881 ggcccgtgcc agggaatccc gggcccgggg atccggcgcc ggaagctccg gcggggacgc
     8941 cgcgaaccag ggttcctcgg ggaatcccgg cggcaggggc agcggagcca ggccgtccag
     9001 ggcggattcc agggccgcgt aggccgcctc cgcccgggcc agggccggat cgtccacccg
     9061 gatctctcgg ccgggctccg cgacgggcgc cagggccacc agggtccgca gggccgcccg
     9121 ggcgaccccg tcggtccggg ccgcagccgc cagcctgtcc aggacgggcc ccgaggggag
     9181 gacggccacc cggccctccc ggaccgccgt caccgaggcc tcccccgccc ggaccaggaa
     9241 ccgggtgccc cgtaccccgg acaggatccg cggggcggcg accaggaagg attcccccgc
     9301 ggagagggac cggacgtcga agagggcggc gcccgaggaa acgaagacct cgacgtgggc
     9361 ggccccgggg gccaggcggg cggcccggag ctcgacctcg gtatccggaa ggaggaggac
     9421 cgcggccagt ccgcccaggc ggagctccgc cgggtcggac gcgccggtcc ggacccggtc
     9481 tcccagggcc aggagggttc cgggttccgc gggaagccgc tcctccccgc gcaggatccg
     9541 ggctcccgtg tccccgcgga cgagctcggc ctgcccgcct tcggggaaac ggggggcggg
     9601 gacttcggat acggcggacg ccggagaacc gggctcggga gccgcggggg cggcctgtcc
     9661 ggaatcgccg gcccgggagc aggaggccag cacggccgcc gcgatgaaca acaccaccct
     9721 ccgatccacc ctcgccccca aatccggcct ccctttataa ttccaacagg atgaacaaga
     9781 tgttgaatgg atgaacaaga tttggaaccc agaattcttt gatctatacc atcttgttca
     9841 tctcgtattc atctgtagat cctgtcggac ttttcgaacc cgtccatagt atatcccgcc
     9901 cgggagtccg gagcttgccc ggcggacgga atccggctag tatggccccg acggcagcct
     9961 agcagattgt tgaaatactc gaagcatttc aacaccctga tagagcggtg aatcgggagg
    10021 ttgaggggca tgttcgagtt cctgaagcgg atgttcagaa aggccccgga gcgggaaccc
    10081 gggctgatcc cggaggagct gtggacctcg gaactcgacc agcccgcctc cggccgcttc
    10141 ctcccggttt ccgaagaccg ataccgggcc tcctaccgca aggacggagg cttcgagctg
    10201 accctgctcc ggaagaacct cttcgcctgg gcggaggtcc cggagccccg gcacgaggac
    10261 ctggccctgg aggcccagat cgaattcacc ggaggggagg gcctgggcgc ggcgggattc
    10321 ctcctgcggg ccgaggggga ccgggacttc ctgtacgtcc tggtgtcgaa ccggggattc
    10381 ctacgggtgg acgcggtctt caacggcaag ccccaccccg tggtcccctg gaccgagtgt
    10441 cccgagcccg tcggcccctc cttcaacctg aagatcgtgc tgtccgggtc cctcctcgtc
    10501 gccgcggtgg acgacctgtg gaccgcggag tgcgaggacg agaccgtgcg tcagggccgc
    10561 ctggccttcg cggcccagaa ctacgacgag gccggggaag cttcctgcgt cctccggttc
    10621 atcctgacgg aaacccggcc aatggaggtg gaagcccggc gccttcgctg gtccgtcctg
    10681 gagaaggcgg acccgggaca gcgcgtccgc ctggcccgga gcctggcggc ggggggcctg
    10741 gacgtcccgg ccctggtcca gctccggaag gcggagaagt cccgccccct ggacgccgac
    10801 gggctcttcc tgcgggccga gtgctccctg cgcctggggc tactggagga cgcggaaacc
    10861 gcgctccggg cctgcctggc cgcctccggg gacgacccgg gggaacgggc ccggcttgcc
    10921 cgggaggagc tggccaacct cctctatctg cgggggaact atctcgagct ccgggacgag
    10981 ctggccggcc gggacctctc ggccagcccc cgcctggcgg gacttttggg ccacgcctac
    11041 tggaacctgg ggaactgggg cgatgccgcc gactgctacg gccgggccgc ccgggcggag
    11101 ccggacatgc ccatcttcgc cctgaacgag gcgcggagct gggaccaggc cgggcgccgg
    11161 gaggaggccg ccgaggccta cgagcgcgcc gcccggggct tcctggccca ggagtccccg
    11221 gaggacctgg aggaatgcct cggacgcctg aaggccctcc gaccgaggtc cgacgcgacc
    11281 gcggccctgg cggcgaagat gctctttcgg gacgggaaga aggccgaggc ggaaaagatc
    11341 ttccgaaagc tggccgccaa ggggaccgag gactccgcgg tcctctacct cctgggcctg
    11401 atcctctcgg agcgggggga gcgggccgag gcggtggggc tcctgcggag ggcctgcgcc
    11461 ctggagtcgg gcttccccct ctactggttc cgcctggcgg aggccctctt cctctccggg
    11521 gacgccgagg gggccgcgga gcccttgtcg agggcccggg acctggcccc cggggacgga
    11581 tggatcctga acctgggggg gctcctggcc gagcgccgcg gcgactccgg ggaggcgatc
    11641 cggtgcttcc gggccgcccg ggaaaccctc ccggaccagg tcgagccggc ggtcaaccta
    11701 tccgaggccc tcgacgccgc gggatccacc gccgaggccc tggagatact gtccggcttc
    11761 ccggactccc ccgcggcccg caaccaggcg ggcaacatcc tggcccggcg gggacggctg
    11821 gaggaggcgg cggccgaata cgaccgtgcc gtgcgcgccg ccgcgggaac ggcgtccccg
    11881 gagatcgagg cggagtaccg ggccaactac gcggcctgct gcctggagct ggaccggtac
    11941 tcggacgcgg aggaccagct ccggcgcgcc ctccagcgtt ccccctcccg tagggtctac
    12001 ctcctgaccg gggacctggc ggcccgctac ggggaccggt accgggcgga gacagcctgg
    12061 atggcgggcc tggagctctt ccccggggac ccggacctcc tggagcgcct cttccggcac
    12121 cgtctgtacc ggggcgccct gggggaggcg gaacagaccg cccgggaact cgagcggtcg
    12181 gatcccgccc gcggggagcg ggcccgggcg gagctccggg acgcgacaac ggtccgctac
    12241 gagtgcgcct cctgcggccg ggaatggagg gcccccaagg acctgcccgc ccgggccgcc
    12301 aggaccatcc gggcccagcc ccccgacgat tccccggccg gagcctgccc ggactgcgga
    12361 aaggtgtact gcgtggggtg ccggaaggac cacctggagg acctgcgctt tacctgcccg
    12421 ggatgcggga ccttcctgaa gctctccgac gaccggctcc ggcacctggt gctcgagagc
    12481 ctgggaggga agcggaaggg atcccggggc gcgtaggagg cgcggtttcc gcccgccttg
    12541 tctttttcac ccccccggga tactataggc ggacggcgtc gccgcaggaa tcggacgccg
    12601 gaaatcttcc tgtaggaccg tcccgtgaag cgcagcatcc tttccctatc gatagccctg
    12661 ctcctggccg ccgcgacccc ggcccaggat atcgtcacgg cggagcagta ctttgccgga
    12721 gtatccgaac ggtacgccca gatcacggat tacgaggccc ggatctcgat ccggacccag
    12781 cgaaccacca tgctgggcac cgtatggtac aaggccccga ggctcctccg catcgacttc
    12841 acccagcccg cggaccaggt gatctgcttc aacggggaga acctcgtggt ctacgtcccg
    12901 gagtaccggg ccgtgctctc ccagtcggtc tcaacgagcg gctccggcgg agccggcttg
    12961 gccaccgcgg acggcctccg aaccctgcgc aggaactaca ccgtagccta cgagcagacc
    13021 cccaatcccg tccccctgga ggagggcagc ccggagctgg tggtcaagct gctcctgaac
    13081 cgccggacag tggcggaggg attccggacc atcgtgctct ccgtcaaccc ggatacccgg
    13141 atgatccgcc ggatctccgg catcaccgtc tccaacgaga ccttcgtctt cgacttttcc
    13201 gggatccgaa ccaaccaggg gatcccggag acccggttcg tatacgactc ccccgcttcc
    13261 gcgaacgtgt acaacaattt cctcttcaaa accgaaaact gaaaaccagg aagaatgcga
    13321 tgatttccat cggcgaacaa ctgaaggcgg ctcgggaacg gacggggctg gacctggatc
    13381 gcatcagcga cgataccaac atcgccaagc gtttcctggc cgccctggag gaggagaatt
    13441 tctccatctt ccccggggat ccctacgtga tcgggttcct ccggaactac gccgagtatc
    13501 tcggcctgga cgcccgcgag ctggtcaccg cgttcaagaa cgtccggatc caggagcagc
    13561 ccatccctgt ggaatccctg atccccaagc gcggtccgtc gccctggatc ttcgcggcgg
    13621 gagggatcgc ggtcctggcc gtggttgtcg tcctcttcct ggtcctgggc cggaaggatc
    13681 cggcaaaccg gggcgccccc gaggaggccg cggtccggac accccaggag tacgccatgg
    13741 acgtcccggt cctggagaag cggttctacg agggcgacac ggtcctcatc cccctggggg
    13801 agcagaaata ccgcctcctg gtggcgaaga tctccgaccg ggtggccgtg gagtcccccg
    13861 tcgggcgggc cctgttcatg ctcggggaaa agggaaccct ggacctggac cgggacaccg
    13921 cgccggagct ccagatcttc gtgaacgact tccagaaggg ggatccctcc aagggcgccc
    13981 tgatcaccct gagcgccgag ggagccttgg ccgcggtcct gggcgctccc ggcgtcgcgg
    14041 cgcaggccga ccccgcggaa accccggacg cgcaggccac cgcagcggcc cccgccccgg
    14101 agaccccgat caccccggac ctcaagacca ccgtgatctt cgacaaggtc gcctcgccct
    14161 tccccatcgt gctgaccacg gtcttccgca actactgcat gttccgctac gaggcggacc
    14221 gccgggagcg ggaggaacgg tactaccaca agggcgacca gatcaccgtc aacgtcacga
    14281 acaccgtgaa attctggacc tcaacgccgc ggcctccaag accacggtcc tggcgaccgg
    14341 gggccgctcg gtggacctgg acctgggcgg ccagggggag gtcgcggtca agcagatccg
    14401 ctgggtcgcc gccgagggcg gcgggtggaa cctggtcctc gtggatgttc cgtaaggaaa
    14461 ccgtgaaccc cgcatccccc gaaacgcccc ggaccttctg gatcgacctc cacggctgcg
    14521 ccaagaacca ggtggacgcg gaggagatcc gcgcccgcct cgaggccgag ggctggacag
    14581 cccgggagag ccccgaaggg gccgggatcc tggtcgtcaa cacctgcggt ttcatagaat
    14641 cagccaagaa ggagagcctg gacgccgtcc tggacctgaa ggcccggtac cccggctccc
    14701 gggtcctcct ggccgggtgc ctggcccagc gctattccga agccctgtgc gcggacctgc
    14761 ccgaggcgga cgggatcttc gggaacgggg atctatcgcg ggttaccgag gccgcggaag
    14821 ccctgcgccg gggaggacgg cccgtcctgg ttcccgggac ggcgcctccc gcccgggctc
    14881 cccgggacac cctcttctcc tacccccgga gcgcctacct caagatctcc gaaggctgct
    14941 cgaaccgctg ttcctactgc gccatccccc tgatccgggg ggacgcccgc agccgggatg
    15001 tagccgacgt ggccggggaa gcccgggatc tcgtgtcccg ggggatcttc gagatcaacc
    15061 tgatcggcca ggacctgggg gcctacggac tggacagtat cggacggcag gctctcccgg
    15121 acctcctaac ggaactccgg cgcatcgacg cggacttccg ggtccgcatg ctctacatcc
    15181 acccggacca cttccccggg gcgatcctgg accactgcgc ggcggatccc cggatcctgc
    15241 cctatttcga cctccccttc cagcacgcct cccggtccgt cctttcggcc atgaaccgcc
    15301 ggggcgacgc cccgacctac ctgcgcctga tcgacgggat ccgggaacgc ctttcggacg
    15361 ccgtcctccg ctccaccttc ctggtgggct tccccgggga gaccgaggag gacttcgcgg
    15421 tcctccggga cttccaggac cgggcccggc tcacctggct gggggccttc gagtactccc
    15481 gggaggagga cacccccgcg gccgccctca agggccgggt gccccggaag accgcctccg
    15541 agcgccgccg cctcgtggag gaagcccagc ggcccatcac cgaggccgac ctgcgccgct
    15601 tcctgggccg ggacctcgag gtcctggtgg aggagcccgt ggagggcgag gagctctccc
    15661 tgggccggag ctacgcccag gccccggagg tggacggcct tgtggtcctg aacgcccgcc
    15721 tggacccggg ccgggtcgtc cgggcccggg tcgtcgccgt gaacggggtg gatctggagg
    15781 cggcccttta catccccccc tcccccgctc catagtcgac cccatgcccg agctccccga
    15841 gtacctgcag gacctcaacc ccgaacagct ggccgcggtg cgccacgagg gggggaacct
    15901 cctgatcctg gcgggggcgg ggtccgggaa gacccgggtg atcaccacca agatcgcctg
    15961 gctggtccgg gagcggggcg tggccccgga gtccgtcctg gcggtcacct tcacgaacaa
    16021 ggcggcccgg gagatgcggg accgggcctc ggccatcgac cccgagtgcg cccgggccta
    16081 cctccggacc ttccactcct tcggggcctg gttcctccgg cggaacgccg cggcggccgg
    16141 cctcgacccg aacttcacca tctacgacga cgacgactcc acgaccctcc tgcacgggac
    16201 cgcgccggac ctgacccggg cggagtgcgg ccgcctggcc tcccggatcg cccgggccaa
    16261 ggactacggc ctggccccgg acgccccgga cctggaggcc cggttcccgg accggaactt
    16321 ccgccgcctc tacgcgggct acgaaaagcg cctccgggag accgggaacg tggacttcgg
    16381 ggacctcatc ctcctgcccg cccgggtcct ccgggacgac gaggccatcc ggaaccgctt
    16441 ccaccagcgc ttccgggtga tcctggtgga cgagtaccag gactccaacg tggcccagtt
    16501 cgagctgctc aaggtcctgg ccgggggcgg ggcctacgtc tgcgtggtcg gcgacgacga
    16561 ccagtccatc taccggttcc ggggcgccga ggtgcgcaac atcctggagt tccccgaggt
    16621 cttccccggc accgagatcg tacgtctgga gcgcaactac cggtccttcc agagcatcct
    16681 ggacgtggcc ggggccgtgg tggcccggaa cgagggacgc ctgggcaaga ccctgaaggc
    16741 cgtcaaggcg gggggcccca aggccaagct ggccctgctg gcggaccagg acgaggaggc
    16801 cgcctacgcc tcccgggtgg cggaggcccg ggcgtcccgg acgggccgct ggtcggacac
    16861 ggcggtcctg taccggacga acgcccagtc cctggccttc gagcacctct tcaacaagcg
    16921 cggcatcccc taccggatcg tgggctccgt acggttctac gagcgggagg aggtcaagga
    16981 cgccctggcc tacctggccg tcctgtccaa cccccgggac gaggtcaatt tccgccgggt
    17041 ggtcaacaag ccctcccggg gggtcgggga taccagcgtc caggccatcc tggaggcggc
    17101 ctacgcctcc gacggcgacc tgctctcggc ctcccgcggc gcgggccccc tgctcaaaac
    17161 cgccaagtcc cggtcggggc tttccgcctt cgtctccctg gtcgacggcc tgcggggcct
    17221 gctggacgcc ccgggggagg gccgtcccct ttccggcctg gtggagcgga cggtcaagga
    17281 ctcgggcctt gcggagtacc accgggacca ggacgagatc gcggggaccc agcgggccgc
    17341 caacctggac gagctcgtga acgcggccgc ggacttcgag ctctccccgg cgggcctggc
    17401 ggagttcctg gagaccatcg agctggaccg gaggctgtcc gaccaggagg agagcctgga
    17461 ggcggtgacc ctcatcacga tgcacaacac caagggactg gagttcccgg tggtggtggt
    17521 cacgggactg gagcagggcc tctttccccg ggacgacgac gagggggagg acctggagga
    17581 acagcgccgg ctcttctacg tggccgccac ccgggccaag gaggagctcc acctctgctc
    17641 ctgccgcagc cgcctcctgc ggggacggcg ggcggacttc ctcccctccc ggttcatcca
    17701 cgagatcccc ccggggctct acgagaccct gggaggccgc ctgcccgccc ccgcccccga
    17761 gggcccctgg aagccgggac ttccggtcta ccacgacgac cacggctcgg ggtacgtaat
    17821 ccaggtgaag gcgggccccc cggactcggg gcccctggtc ttcgtcaagt tcgagacggg
    17881 acgggtgctc cagttcttcc ccaagttcac ccggaaactg gaggccctga agtgaagacc
    17941 gacctgcccc ccgtccgtcg catgcggctc tggcggctct acgccgagga cggccggcgc
    18001 tttttggacc tccggctcct ggacaaccgg ggcttcctgg gggccaaggg ccgcttcgcg
    18061 ggcacccggg ccaagaacgg gatcgacctg ggcctgctca aggaggcccc cggacggcac
    18121 gccgagcgcc tgcggaaggc cctggcggcc gagttccccc gtctcgcggc cttccgcttc
    18181 ttccggaacg aggagcgggc cctggcgacc ctctcgcgga cccgaagcgg cctgtacgac
    18241 cctctggccc ggcatgccga ggcggaagac aggccggccg ccggggcggg aaggtccccc
    18301 gccgcgggaa tggccgcggc cgagggagcg cccgccctcg cggtccaccg cttcttcctg
    18361 gccgaggagg accgggcccg tctcgaggcc ttcccggcgc tgctcatacc cctcccctgc
    18421 cccgagccct tcgggcccgc ggtcctgggc ttttccgaag cggaggcggc ggcctccgtt
    18481 cccgaagacg agcttccccc gatcatgccc ttcacggcca tgcgggcctg ggaggatttc
    18541 cggggcatga gcgtccgtct gaccgggggg cgccgggccc ggagcggaga gggaaccccg
    18601 gcctacgcgg aggacctgtg gaagcggtcg gatcgcaggc tcacgccttt cttcgaacgc
    18661 cggggaccct acctctacgc ccgctgcgcc cgggcggact gggagggctt ccgggcccgg
    18721 gccctggaag cgggctgcgt acttacgccc gaatgggagc ttccttccat cgtgcccggg
    18781 gacttcgacg acggggagtt gaagaacttg gctaaagctc tggagatact ctaagcccgc
    18841 cgggcggcgg cctggatcaa gaccacggcg cccacgatca gcacccctcc cgcgatctgc
    18901 tggagaccga agcgctcccc caggatcagg gcggacagga gcaccgcgat caggggttcc
    18961 accgtggaga ccagggaggt gttggaagcc ccgatcctct gcatggcccc gaagagggtg
    19021 acgatgggga tcaccgtggc cgccaccgcg attccagcca ggcccaggac cgaggagagg
    19081 gcctcggggg ccttgaagcc tcccgtcagg gccgtaaggg tccagtaggc cgcggccgcg
    19141 gcggtcatga tgaaggccgt ggagtgcacg ggcttccgac cctcaagaac cctctccccc
    19201 acgaccaggt aggccgcgta ggtcagggcc accaggatgc cgaagcctat cccgatcgcg
    19261 gggtaccggc cggcccggaa gaaggtggtc acgcagcccg tcagggacag ggccagggcg
    19321 gccaactgga cacccgtggg ccggcggccc aggaagacgg cggacaagag aaccacgaag
    19381 gacgggtaga ggtagaggag cagggaggtg atcccggcgt ccaggaagcg gagggtcacg
    19441 aagtagagcc ccgcctgggg cgcgaagccc aggagtccca gggccaggac cgggaggttg
    19501 ggacgcaggg ggggcagacg ccgatccgcg gccaggatcc agatccagag gaaggccgcg
    19561 gccccggtga agcgccaggc cagggcctgg gaggtggaga agccctcggc gtagatcagc
    19621 ttggcgaaga tgcccagggt ggaaaagctg gccgccgaga gcagggccag gaggaccccg
    19681 gtcccggctc cccgggaggg gtggaccggg actgccgccg cttccgtccc cggcgcgccc
    19741 ccggccgtct ttgtcgtgtt ccgcatgatg cttcctttcc gacgcgcgac ggatccctca
    19801 gcctgcgccg gggccttccg agtcgaagac cgccccgccc agggcgtagt cctcgggctt
    19861 gacggccctg cgggcggcct cgacgtccgc ctttcggcgg gccagctccg ggaaggacca
    19921 ggcgccgccc gcgtcgggct cgccgccggc ccaggcggcc agctcctcca cggccttttc
    19981 cgcggccttc cggtccgcgg ggttcgccgc ctcgtccggg accgctgcgg ccccggggct
    20041 cacccgcccc ggctcgtccc agacccgggc gtagaccagc ttgcgcaggt tctgccgctt
    20101 gatctcctcg ttggtccggg ccaggcgcag ggagcgcagg aagggtacca ggtagaagag
    20161 gaccgcgaag gccgcgggca cgaagcccag gccccagagg atggcggaca gggggtccgc
    20221 ggcgacctgg gagatcaagg cgtacacgat caggtagaag tccgcgggta tccgctcggc
    20281 cccgggcggg agacggggaa tgccccggga cagggcattg accaggaaga aggaaccgaa
    20341 ggccaggttg accccgttca gggccgcgaa ccagacgttg gccttccggg ggttggagga
    20401 gaagggcacc aggcgcttca ggggggccga gccgccgaag gtccggtccc gctcgtcctt
    20461 cctccgcatc agggccggga agaagtagta gatcgtgccg ctcggggtga cttcggggct
    20521 gccctcgaac tcgtagaggt agcggtttat cgcgtcctcc gcctccccgg gccggtgccc
    20581 ggtcaaggtc atgaactcct ccagggagat cagcccccgg ttggcctgga tgaaggccgt
    20641 caccgccttc ttctcggtct cggcccaggg ctcgttgggg tccccgtccc cgaagacgaa
    20701 ggagaagacc gccttgtgca gggggcgccg gtccgcggac cgggccaggc gccgctcccg
    20761 ctcgtggtac cgctgctccg ggctcttgaa gagctcgccg tagaaccaga tccgcacgaa
    20821 gagctccagg atccggcccg tgaacatcaa gcctcccccg cccccccggt tccggccccg
    20881 ggagtcccgg ttccccgcgg tctgggcggc aatggaggcc gccaaggcgg ccagggtcag
    20941 ggccaggaag aggacgaagt agcccaccag catgaccacg atccagatct tgaagaggaa
    21001 ggccgcgacg gccgcggcgc ccttgcgcag ggctttccac aggcgcttca gggtcggacc
    21061 gaggccccgg taccggctgt ggaggccccg ggggaaggag tagaggatct ccccgctctc
    21121 ggtcacccgg agacgggccc cgaactcgtc cgccaccgcc cggacctcgg tctccacctg
    21181 gtacttgggc aggcccgtgc ggaccaccag gtccgccacc gtggcttccc cgcgggtatt
    21241 ccggaactcc gaggccagac ggtcccgtac ggcggcggtc ttgtagtcgt ataccttcgt
    21301 ttccttggcc atcctgatct cctagcccgt cagtcgaccc gcacccgcct gagagcccgg
    21361 gcgtagagct ccccgtactc ccccgcggcc cggtcccagg tgaactcccg ggtcatggcc
    21421 cgcttccgca tggccgcgat gtggtccttc ttgttgtagt acgcccagac cgcccagccg
    21481 acggtgtcgt agatcgaccg gggggtcaaa aggtcgaaga ggaagcccgt cccctccccc
    21541 gtgtcttgcc ggtagttctc caccgtgtcc gcgagtcctc ctgtgcgccg cacgatgggc
    21601 agggtcccgt agcgcaggga gtacatctgg ttgagcccgc agggttcgta gcggctgggc
    21661 atgaggaaga aatccgagcc cgcctcgatg aggtgggcgg tccggtccga gtacccgatc
    21721 ttggcccgga agttgggaat ccgggcggcc agggcctgga cctcgtcctc gcaccagcgg
    21781 tccccggagc ccaataggac aacctgcaga atcatgtccg tgcagatccg gtaggcgctg
    21841 ccgtacaggg gtccgaagag ctccccgatc cccttctggt ccgtcaggcg ggtgaccatg
    21901 ccgatcaggg gcacgtccgg gtcctcggga aggccgaact cacgctggag ggccgccttg
    21961 cacgcggcct ttccggacag gtccgcggcc gagtaatggg cgggcaggtg gcggtccgag
    22021 gaggggtccc agtcgtcgaa atccaccccg ttcaggatcc cgatcaggtc cgagctccgg
    22081 gaccggagga ggccgtccag ggagcacccg aactcggggg tctggatctc ccgggcgtac
    22141 gtgggggaca ccgtggagag ctggtccgag gaggccagtg cccctttgag gaggttgatg
    22201 gcgtcgtagt actccagccc cgccttgtgg aagaggtccc agggcaggcg gaagtagggg
    22261 aaaatctcct tggagtagat cccctggtag cccaggttgt ggatggagaa gaccgtggcg
    22321 gtccgggcga actcggaagc ggactccacg tagcggcggt agaccggcac caggcccgcg
    22381 ggccagtcgt gggcgtggag gacatcgggg atccagtcca gcttgcggca gagctggaag
    22441 gccgcacggg agaagaagga gaagcgctgg gggttgtccc gaaaatccgt ctcctccttg
    22501 gtcccgtaga tcccgtcccg gccgaaaaag cgctcgtggt ccaggaaata aaccggggcc
    22561 ttgcagcccg ggagagtcga ttcgtagacc gcgcaccact cctcccccca gcccacgggg
    22621 acgcagaggg gcccctccag cttcttgagg gtcgtcttgg gaacgcagta gtaccgggga
    22681 aggacgatcc ggacgtcgtg gcccgcccgg tccagggccc gggtcagggc cccgaccatg
    22741 tccgccaagc cgccggactt cgcgaagggg gtcgcctcgc tggataccat gaggatcttc
    22801 acggagttcc ctcctcggaa tcgggacaag gatcgggatc gagtatagcc agaaacccgc
    22861 ttaggggcaa ggaaagagtt ttagcagggt gctgacaaac tcgaagcttg tcagcaccct
    22921 tctatgcaat tgctcgaaaa gcttgcgctt ctctgcgcaa ttgcgacatt aaggaagctg
    22981 ttgacaaagg cgccatcgcc tttgtcaaca gcctgttagg gaactcacca cggagaacac
    23041 ggagggcaca ggggagaagg gtctagcgga gaattcggac aggatgcaca ggatggaaga
    23101 cggatgaaca ggatagaatc cgagtattga ttctacttaa cctatcctgt ccatcctctc
    23161 caaatcctgt tcatcctgtc cgccctatgg ttccaagacc gaacgcgggg gcctaatacc
    23221 gcaccaccat caccttacgg atctcgtcga ttcccggggc cacgatacgg atgaagtaca
    23281 tcccccgggc cacggggtct ccggctcggt tccggccgtc ccaggaggcc gtgtagtccc
    23341 cgggggtcat ggtccccctc tgcaaggagc gcaccacgtc cccgtccaga gtgaagaccg
    23401 tgatcgtcac cggccccgcc tggcctatcg tgtagtgcag acgggtccgc tcccccgtag
    23461 tcggatcgat cacgttgttc aggatcgaga ccccgccctt ctgggtgacc acgttccgga
    23521 tcaggatccc gaagggccgc accctccgat accagtccgc aggaatggcc gtagcgtccg
    23581 ggtccatgtc cagacggccc gcgtagaggt ccgcgggata cggtccgggt aggcggaagt
    23641 agaactcgaa ccgcgcccgg tcgtagatct ccgggtccga ggccaagaac gagtagtccc
    23701 agatcggtgt agtaccggtc cccgtgagat tcgagacgaa gcctgtgaag ggataggagg
    23761 ccaagcccga gaagtccggc tccgcgtaag tgggcagcca gatccccggt accgcgctga
    23821 gatacgcggc cgagacgtcg gatttataca ggatctgcgg cgccgaaccc agtcccaggg
    23881 cgtcctccac ctgcaggtcg aagtcctggt cccggaggaa ccgggacccg tcgaagaggc
    23941 gaacgaagcc cacgtactgt cccgaggcgt ccagttccgg gatcgtcggg tagtccacgt
    24001 cgggcacgcc gggcacgtag gcctcgtcct tggcccagag gggccagacg aagaaggagt
    24061 ccgcattggc accgcccgaa tagctccagg ccccgaccgt gtccgagggg ggcacggaga
    24121 ccaggaggtc cgagacgcgg tgggtgttgg aggtcaggct gatcgggttg ggtagggagc
    24181 ccgcgggata atcgggaacg gtcgtgatat aggcggaagc caccgggtcg gtcgaccaat
    24241 cgtagagggg caccgcttgg tcggtgatgc ccgcgttcac cgtccgggca agttcgccga
    24301 ctatattggc gatcgaaaaa ttcccgccgt aggcggcaag gactccgtag acgtctccgt
    24361 cgcctatcag acccggcagg gagaccgaca ccgacccggg gatctccgcg gcggttagta
    24421 cgccgcctcc cgctcgttcc accctctcgt tgaaggcgaa gaatacgtcc ggggtggacg
    24481 gtacggcgag ggtataggcg atcctcgggg gtacggtgtc cgcagggatc atcggatccg
    24541 cgaggttcaa caccaaggta tagtcgttcg ggaaggccgc gttgctcttt aaggccgtat
    24601 tgttggtcat ggtccaactg ggggtccgtg aggagtccag gtagggcttt tcgaccagat
    24661 ggatgtagaa gtccgcgtcg ccgacagttc cagaaaaccc ggaaggatac actgtcgtgt
    24721 cgatttcata ccccgtaacg gttatctcca gatcggagaa gtccatattc aaggcaccct
    24781 gggcggtaac ccggatccgg tcgatgcgtc catcgtcgtc cgaatcctcg gtgtagctgt
    24841 acacgaccgt catgccgctg aaccaagcat agcaagtaag accctgttct aggggaggag
    24901 ctccatcggg gacgaccgtc cttagggtga cgtacaagct gttgtaggcg atgggattcg
    24961 atcgggcgtc actataccag acatcgacat attggaagtc aaggttcccc gttcccgtct
    25021 tgtccaactg ccacatcaac accggattgg gaggaacgcc ggtcagccag ttcaggtcgg
    25081 ccccgtcatc cccgagatca tcccgtgtaa tggtcacgtg attcgcgatc gtcccgatgg
    25141 cctggaacgt cccgcccgcg acgatacgct ggatattgct ccgttggaac cgaatcctcc
    25201 cgccgggctg gttgtacctc aggttgtacc actggttcgg cccggacacc gtcgcgagtg
    25261 cggagtcggt gaactcaacg atcccagtgc cgggcgtgaa ggctgtgatg actccggtcg
    25321 cgttggtcca gttcccggcg atggtgatcg tgaaattccc ggacgcgagg gtccccgtct
    25381 ggatcgtcag atcattcgcc aggccgagat tcgcggcgat ggtgatggtc cctcccgcgt
    25441 tggcgatgaa gttccccagg gggatagtgg cgttaagatt ggtcgggctc cctgtcatac
    25501 ggagcgtcga gttgaggggt gtcgcgaagg ttccggagat cgtgacgttc ccgttggtgg
    25561 aaattgtgtt cgagaaggta ttgttgatgg cacccgagct ggtgacgaac cccgccgcag
    25621 tccatgtcgc tcctgcggcg gtgtcgaaag tgatcgtgcc cgtgttcgtc acggacccgg
    25681 tggaggcggt cccgcccgcc aaggtgatga taccggcgtt cgtgacgggc ccggcggaga
    25741 gggagggggc ccccgccccc gtgaaggcga tcgagccgcc ggccgccaag ttcaaggcgg
    25801 aggcgcccgt attgatccta cccgtcgtcg ccgtgacgga gccgttcgtg atcgtgaggc
    25861 cgctcgtggc gttgatcgtc ccggtcgtga cttgggcatc gccgtcggtc agcgtaaatc
    25921 cagtacctcc cgagacgttc aaggtctgga cgcccacatc gaccagcgca cccgctccgt
    25981 ccagtgtgag acctccggag aaggtagcgg tcccgtccag gatcaggata tccgaggggt
    26041 ccagggtatg cgccacggcg gtggagaacc cgctcacgtg cagggtcaaa gcgttcaagt
    26101 cgagttggtt cccgctgagg gtcatcgtgc cgggggagac gatggaaacc gccccggcgg
    26161 atgaaaccac ctgaagggtc ccgccggtgt tgttcagggt tctgccgttg gccatcgaca
    26221 gtgtcgccat gcccgccgcg ttggaggcac caaggttgaa ggtaccagaa ttcgatgaat
    26281 tcaaccccag gc
//
LOCUS       DAOTEI010000031        25180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1287_length_25180_cov_5.316776, whole genome shotgun sequence.
ACCESSION   DAOTEI010000031 DAOTEI010000000
VERSION     DAOTEI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 25180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25180
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_25180_cov_5.316776"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="P5313_04740"
     CDS             complement(<1..790)
                     /locus_tag="P5313_04740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRY79703.1"
                     /translation="MKKVYSRIESITGNVITVRAEDVQYGELAQVSTRFGVSLAEVIR
                     LADDEVSLQVFAGGRGISTGDEVRFLGHPMQVSFTDSLMGRIFNGAGEPRDKGPELSE
                     NLIPIGGPSVNPANRIIPREMIRTGIPMIDLFNTLVKSQKLPIFSVSGEPYNQLLARI
                     AMQAEVDLIILGGMGLKYDDYLFFKDTLEQGGALSKTIMFVHTASDPVVECLMVPDIS
                     LAVAERFALKGRNVLVLLTDMTNFSDAMKEIAITQEQVPSNRGYP"
     gene            complement(792..2564)
                     /locus_tag="P5313_04745"
     CDS             complement(792..2564)
                     /locus_tag="P5313_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRY79704.1"
                     /translation="MTKTNGSVTGVNGNMVTVRVEGAVSMNEVAYIETRGKRLKSEVI
                     RIRGDLVQVQVFEMTKGIRVGDPVAFTGEMLAVELGPGLLGQIYDGLQNPLPEVAAKV
                     GHFLEPGVYLEALPRDRAWAFTPAAKPGDKVRAADLLGTVPEGAFTHRIMVPFGFLGT
                     YTVKSVAPAGSYKVTDRVAELQGEDGTVHPVTLMFQWPVKRAVTCYAERLKPVEPMVT
                     HVRLIDTFFPVAKGGTYCIPGPFGAGKTVLQQITSRHADVDIVIVAACGERAGEVVET
                     LKEFPELLDPHTGRSLMERTIIVCNTSSMPVASREASVYTAVTMAEYYRQMGLDVLLL
                     ADSTSRWAQAMREMSGRLEEIPGEEAFPAYLESVIASFYERAGIVRLADGNTGSVTIG
                     GTVSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARKYPAIHPLESWSKYSGILSAEA
                     VRYAHAVLARGAEVGQMMKVVGEEGTSLDDYVVYLKSEYLDAVYIQQNSFDPVDAAVS
                     PERQKSVFSMVIGVLGTAFRFKDKDEARDWFYKLRQGFLDLNGSEWKSDRFAQVEKGL
                     RTLIGERKTGLDPKAARILETLEK"
     gene            complement(2594..3175)
                     /locus_tag="P5313_04750"
     CDS             complement(2594..3175)
                     /locus_tag="P5313_04750"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HRY79705.1"
                     /translation="MPAYYYLRSTLPTLLPASAPPFSFRGFLDRCEGRMRDADFLVLA
                     AAGDTEAEDAETVRRVRASRVLSSFRTWDKALRNELARLRAHKLGRAPEKFLRPGDPE
                     WDGIRAAQAAAQCEDPLEGELIIEREKWAYLERLEAGHYFDLEALAAYALKLRILERK
                     ARFEAERGEASYRTAYHEILDTAEAPPAEGRGA"
     gene            complement(3180..3794)
                     /locus_tag="P5313_04755"
     CDS             complement(3180..3794)
                     /locus_tag="P5313_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRY79706.1"
                     /translation="MDIRLQELLDKIRKDGIEAAQTDAAKVLAEAEERRRAVLAEADR
                     EAKALLAKAKADADRFEEAGKAALEQAARNLVISFRTEIEAVLAAIVRTDTEKALSGP
                     VLEAAIPAVLAAWKDKGTDDLAVLLPPAELSKLEASFRKKLESELKKGLELRPFPGIK
                     AGFRIAEKGGAAYYDFSADSLAEMISQYLNARLGQIAAAAIRKE"
     gene            complement(3820..4185)
                     /locus_tag="P5313_04760"
     CDS             complement(3820..4185)
                     /locus_tag="P5313_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79707.1"
                     /translation="MERKCLYFLKILRAELEDLLEDLKSVEGRYRERFASSEITNYVL
                     LENEALLSREEESVRALISSIDGMNSSRYKSVDEIVEDLDAVAREFITSKEYPAAVHK
                     LLRRKMDKVLSYVMAEDAP"
     gene            complement(4421..5305)
                     /locus_tag="P5313_04765"
     CDS             complement(4421..5305)
                     /locus_tag="P5313_04765"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRY79708.1"
                     /translation="MRARPERMTDHFELEDLKFFEPDGKVGLLATLDPEGLPHVTLVT
                     AMQAQDERHLIFGQFCEGRGKRYAEERKKVGFLLMTLDRRLWKGTALWTGKAQSGPEH
                     ELMNRKPMWRYNSYFGIHTVHYLDLISVTPGRDLPLAAVARGVLAGALRGSLGEGRIR
                     GTELGRGTELERPDPINPWTRAFIRKTGNLKFLSYVAEDGFPRIVPQLAATCPDGRSI
                     LLPGTEFPADLARIPAGAATALFAMSLDMEDVLVRGRFEPRRRGGVIRVDWVYNSMPP
                     VAEQIYPPVDLARKVEAF"
     gene            complement(5281..6330)
                     /locus_tag="P5313_04770"
     CDS             complement(5281..6330)
                     /locus_tag="P5313_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRY79709.1"
                     /translation="MSRSERGLVLEIQRMSTEDGPGLRSTLFLKGCSLRCRWCHNPES
                     ISPKPELRWSGSRCLGCGECLSACPNRALSLGAGIRIDRGSCDGCGECERTCPGGALE
                     LWGRSMTAEEAVAELLRDRSFFGPDGGVTVSGGEACLQASFVQETLGRLRSEGVGTAL
                     DTCGMCTEFRLLRSAEHADLVLYDIKDADPDRHLRNTGGDLAVVLRNFRLLARPSGDR
                     ARKRIWVRTPVIPGLADSPGMIEGIARFLVESGAESVERWELCAFNNLCREKYRQLGE
                     EWELAASPLMDADHMDRLVDAAIRAGWPADRIRWTGMTAAGGTVGKERLGSPEPGIGR
                     RSWNEEAPCARDRNG"
     gene            complement(6317..8818)
                     /locus_tag="P5313_04775"
     CDS             complement(6317..8818)
                     /locus_tag="P5313_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRY79710.1"
                     /translation="MKTTDSPSAIKQPAGLSPRIRWLRDYYFRGTSRAWNNQQISFTT
                     GTAWDIQYNECNYHIVPETYAFFQTFTSSFRQIARPVALPPDFWNLSLPERRAWFNKE
                     VMTRYLPQEILPRDLIAGARFNVHTSLCLTKREHEERSAVLYGPSGLRKAAIGFHNHG
                     FGNAGPTGGHLIPDYGRVLAGGFEGILAELEGNLAALSPEDREGPRGAQLRAMITACG
                     MPVELAGKYRDEARRLADVERDPARAAELRRMAANLERVPAKGARDFWEAVQALWLAH
                     MLVMSDENYPGPGVSFGRLDQMLLPYWEASVREGMDREFAKEILKCFWIHANTAYDSM
                     IKVGNQGITAGFGQLFNLSGLGPGGRDMTNDLTFLILEVIDEMSPILEPKPNVRLHRG
                     SPEALLDKVVDMIASSQGAPFLLNFDERSMAGMMRQARMSGAEDRIHEGNVHEYASVG
                     CLENTMVGNDRSGTVDCNLNLLKAVELTLGNGYPLVPHTDAITGVTERPRRQGPSTGD
                     PRRFGDFDRFWDAYAEQTRALIREITGLYERGESLRARFHPTPYLSTLVRGCAEKGKD
                     VTRGGAELGYVTIEGVTFATTVDSLLAVKYLVYDNRFCTMDELIRSLKDNWKGYEILQ
                     ARARNRAPKYGRDDPEADELARRVMDLWTGETWKYRTESTGRRFRPGMLSWNYWVGDG
                     FILPASPDGRPKDQFLSNAICPSNGADIRGPTANANSVGTVLGGRGEGGDWGGYFNSL
                     PNGASHTISFNPSLIKRPENRAKFKSFLRGYAENGGTALQVNMLDPEVLRDAQRNPSE
                     YRHLLVRVTGYNAYFTSIGRELQDEVIARIAHEQI"
     gene            complement(8787..9560)
                     /locus_tag="P5313_04780"
     CDS             complement(8787..9560)
                     /locus_tag="P5313_04780"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY79711.1"
                     /translation="MRKKLNIATKKLEREILAGTFKPGDRLPAERALSLRYGVSRSIL
                     REGIRHLSSMGLVRTEAQSGTYVTDYRKEASLDFLLYLLDNNEPLDPELFRAINELRE
                     ILESGCASRAAARSDPERVRALRSALAALRAEEGDPEGLAAKDYEFHALFVEQTDNLA
                     LQLLFHAFRSVYLYYARCFYAEPESASATFSQLECFLDRVEARDESGAAAALSLALAY
                     GRDRVYRALSLDSNAPAISTARSNLKEVPHEDHGFPVRD"
     gene            9673..10413
                     /locus_tag="P5313_04785"
     CDS             9673..10413
                     /locus_tag="P5313_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HRY79712.1"
                     /translation="MTNGLPWLMILPSGIVLGFLLHWLTIRGIDTAEGRLPHCRGAYL
                     AFSAQLLTFAWAVYFTAGLGRYGAVLLPLGMTCSFAGDYFNLQFPAIRNRVREPVFLG
                     ILSFAAAQVIYIGAFLSLIPFRELAERGYFVPILAVLLIAPAMIFRLRVYQPGRPRSL
                     MLGGFLYGFALGTMTAIALSAAVTRGGSWIPVAAGALFFLLSDAVMGETTLHGRHPKH
                     EYQVPWGTYLAAQGLILAGTASILMAPG"
     gene            complement(10460..11455)
                     /locus_tag="P5313_04790"
     CDS             complement(10460..11455)
                     /locus_tag="P5313_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY79713.1"
                     /translation="MEYRYLGRSGLRVSPLWLGTWNFGDPTPEAEAIRMIHRALDAGI
                     TCIDTADSYAAGESERILGRALGGGLRDKVVLATKFHFPVSDGPNDRGNSRRHILRAV
                     EASLRRLGTDWIDLYQAHRPDFGVPQEETLRALDDLVRQGKVRYVGSSTFPAWMIMEA
                     LGISERLGLPRFVSEQPPYNLLDRRIENELVPLALRHGVGILAWSPLAAGQLAGRYAD
                     AARPPADSKAARVSYVAARLTERGIKVARSLAGLAADRGLTPSQLALLWVKDQPGIAA
                     ALLGSRTEAQLEEALPVLNLSLDAETAAALDSLVPPGSAVADFHNTSGWMKMKLL"
     gene            complement(11470..12237)
                     /locus_tag="P5313_04795"
     CDS             complement(11470..12237)
                     /locus_tag="P5313_04795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019505458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY79714.1"
                     /translation="MKAWYEALFENYGRKYDAENFTRGTSGECDFIEKELGSDRSKRI
                     LDIGCGTGRHTLELTARGYAVIGVDLSESQLERARDKARERGLSVEFRREDARNLPFE
                     AEFDAAIMLCEGAFPLMETDEMNFEILRGAARALKPGGILIFTTLNGLFPLVHSVEVF
                     CSSNSEPGNAVYRNNTFDLMTFRDHNVTEVVDDLGNTKTLECDERYYVPSEITWLLKT
                     AGFTDIGIFGAHLGAFSRDHRLTTEDFEMLVTARKAP"
     gene            complement(12357..14993)
                     /gene="dnaE"
                     /locus_tag="P5313_04800"
     CDS             complement(12357..14993)
                     /gene="dnaE"
                     /locus_tag="P5313_04800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRY79715.1"
                     /translation="MSLLEAARGRAYALLAPPVSRAVKGARLSWRSLSGTGAPLLATG
                     EVRFLRPSDREVQRLLVAIGSGRTTFEVQEGELSAPGTILSSPLDAIRPYQSVPEAIR
                     TNEAVVESIELCSLFDGYVFPEYKSCDGDRASAVLRSLVYQGAAKRYGDIDGIVRSRL
                     DYELGIIESKGFSAYFLIVRDIVRKSSRTCGRGSAAASVVSYALGITDIDPLAHGLYF
                     ERFLNPGRKDPPDIDVDFAWDERDAILESVIREYGEDRAARVSNHVCFQARSALRETA
                     RAFGMPDGEIGAVERALYLDRGRTLAEADPVWKEILELAPRIAGFPRHLGVHSGGLVL
                     VPGRLSDLVPVVPSGSGIRVTAWDKEGVEAAGLVKIDLLGNRSLAVVRDALANLAENG
                     TPIAEEDWNPLSDADTIDLLSRGDTLGVFYVESPAMRLLQKKTGKGDFEHLVIRSSII
                     RPAANRYINEYIDRLNGKTYEPLHPLLDDQLAETYGILCYQEDVSKAAVALAGFTPAE
                     ADGIRKVLSKKDVRTRLAAYRAQFMSGSRARGVDDKTIVAVWEMIESFAGYSFVKAHS
                     ASYAKLSFRSAYLRAHHGAEFMAAVLSNKGGFYSTLAYTSEARRMGLRILAPDVNESE
                     IRCRGRDGTIRFGLGMISSLGSETAAGIVAERKSNGPYRSVDDLGCRVSIDRTEAEAL
                     AGSGALDFLDEGQSCSQKLLRLLSRAAAREEPCSRGTELFGEPTPGTELNYRRGHVPM
                     PEHKRLEAQMRYLGTTLDVHPLTLCSQALKRSRTLGKDLPDLAGRRVELAGWPITAKP
                     VLTSADEPMEFVSFEDETSLYEAVLFPDAYLRYRFLLHDGKPLWVRGRVQEDRGATVL
                     SVESLAAMECSVPRGVGRFSVP"
     gene            complement(15429..16709)
                     /locus_tag="P5313_04805"
     CDS             complement(15429..16709)
                     /locus_tag="P5313_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79716.1"
                     /translation="MGTGTVLHVNVIGLMAALEENLDSTLRGRPFVVANRAAPRAVVL
                     DLSREAHREGVLRGMLLSRAVAMLSGLIVREPRTDICARANGELDRICRSYSPQVEMA
                     GNGHVFADLAGTSGLNGPPEDVSQRIRKDIFSATGLRPVLALAGSKTVSKVATRVLRP
                     AGFIALSPWEENQLMRHQPVEFLPGVGAALSRRLDLLGIGEIGDLAGLSDAEARALGP
                     RGPELTARARGMDAVPVDPEPPERRTERGAFIFEPDVVDAELLNPRVDMLASELAYSI
                     RRKGMGARNIRVEVWYADGLRNAATARSPRVLVRDDEVIHGVRDALRRARTRRVRVRR
                     ISIEFSGLGSAGPELDLFEPEETRLARLQPALDRVRTRYGFSAVSPAIILAAGRTGTN
                     GGAVDRSSVPISSLVLCQGVPGEAGDDLHGLAVG"
     gene            16935..17420
                     /locus_tag="P5313_04810"
     CDS             16935..17420
                     /locus_tag="P5313_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79717.1"
                     /translation="MESDIGEIRRDKVQDRRKARQAGGIEDAKAHIPCSQGLDNPSAV
                     PLGITQLDNQRNSLEFVQQASEVHPFAGGIMERRGKLKQDRREPAGPDQRIDSRPKLA
                     GGGGKRAGIPFMGEVPVGLHREIDRPPEASKHSVEDLRSRLAIIREVDFKGVELLAIE
                     P"
     gene            complement(17583..18200)
                     /locus_tag="P5313_04815"
     CDS             complement(17583..18200)
                     /locus_tag="P5313_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY79718.1"
                     /translation="MNTESFCGSFKGQSLIDASEIKNPGRIRGMRKPRLLKESALYHV
                     SARANRKEHILDEEGMKDVFLQVVRRARKRYCFRIENFCVMGNHYHLMIRPGPRESLS
                     RIMQWIMSVFAMTYNRAHGYSGHVWGERFFSKIIEGFREFLEIFRYIDRNPVNAGLVL
                     HDWEWKHGGLWHDRLGYRHVLVKPPCEALLWAPRHELLRLPDRSP"
     gene            complement(18295..18366)
                     /locus_tag="P5313_04820"
     tRNA            complement(18295..18366)
                     /locus_tag="P5313_04820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18331..18333),aa:Gly,seq:tcc)
     gene            complement(18501..20240)
                     /locus_tag="P5313_04825"
     CDS             complement(18501..20240)
                     /locus_tag="P5313_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY79719.1"
                     /translation="MDAKGEMEKQISFLYRDTGKDPEAKVLIDQVLARHRDGLRNTDA
                     HRKGSEPTLLSEGDVFLITYGNTLEGRTGEVPLAALRRFAERRLSGVISYIHILPFFP
                     FSSDDGFSVMDYRAVDPDLGGWEEVGALGKNFRLVFDLVINHASSQGIWFQGFLKGDP
                     KYEGWFLTRPKGYDASRVVRPRTHPLLTPYVRTDGSRVHVWTTFSEDQVDLDFSDPEV
                     LAEMLDVFLDYVEKGARIVRLDAIAYLWKEDGHPCLHHPRTHAVVKLFRAVVDALELD
                     VRLLTETNVPHPENVAYFGSGDEAHLVYNFALPPLTLHAFATGDASHLSAWAQDLRAP
                     SGCAFLNFLASHDGIGLTPARGLVDEAAFRQTLDKELGRGCLISYKATPEGPVPYELN
                     ASWFDAIADPDLPEDLRIQAHLASHAIAASLDGMPAVYIHSLLGTPNWAEGPVVRGYN
                     RAINRRVLDLNTVESTLMDPGSRPSRCLSGFRSLYSCRGREPALAPGAPSRVLSRDGP
                     VFSILRGGPEKRILCAVNVSPRPARFETGTNLGLPGRPYDPITGEVISWPCGSAYLDL
                     PPYGVIWLPLDPP"
     gene            20358..21254
                     /locus_tag="P5313_04830"
     CDS             20358..21254
                     /locus_tag="P5313_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY79720.1"
                     /translation="MEQITNGKKILIIGGVAAGATAAARARRLDETAEITIVEKGRYV
                     SFANCGLPYFISRDIPKRSALILQTPEGFFSRYRVTVLLETEALSLDRAARKVRVRGP
                     EGESDLTYDSLILAQGGTPVFPSIRGLDSPNVFKLWTIPDMDAVHKFVETEKPSSAVV
                     IGGGFIGLEAAEAFLKRGLATSIVELTETLMPPADPEFGMLIRRSYEAAGARVFTGRS
                     VRSVDVSSRTVLLDDGSEVPAGIVLVSAGVRPNTELAKAAGLEIGSSGGVSVDEGLRT
                     GAPHIWPPGIWLKSPTGSAAGA"
     gene            21251..22030
                     /locus_tag="P5313_04835"
     CDS             21251..22030
                     /locus_tag="P5313_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY79721.1"
                     /translation="MRIPLAGPANRQGRIAATNALGGSLTYRGALGSSVFKSMEETFA
                     MTGLSEKAASAAGFDVGTSTVSKAHHVTYYPGYEDLFLKLVFDRADGRLLGGQAYGRE
                     GADKRIDALATALAGGLTVDDLAELDLAYAPPYNSANDPINMAAYAAQNDLSGYSPAR
                     SARQAADALGQGEVLLVDVRTYGEYAKGSVQASLHLPVDELRDRLDELPKDRDILLLS
                     KAGFESHIALRQLRQRGFERVWNISGGMAALSLQPGLRWAE"
     gene            22065..22340
                     /locus_tag="P5313_04840"
     CDS             22065..22340
                     /locus_tag="P5313_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY79722.1"
                     /translation="MANIVQDKIASGAFILDVRTLEEFDDEHYPNAICIPVNELPYRL
                     SEIPKDRSVIVYCASGARSAYAARILKASGYPDVVNAGGLYDLPAAV"
     gene            complement(22300..23277)
                     /locus_tag="P5313_04845"
     CDS             complement(22300..23277)
                     /locus_tag="P5313_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRY79723.1"
                     /translation="MATTPESCRIPAGRLLFLDLETTGLSGGAGTIAFLAGLGRVVPG
                     GEAEGFEIRQYLLIDYPGEDAFLDALLGALVSETVLASYNGRSFDEPLLRTRCILNRK
                     HWPPHPHADFLPAARRLWRKRFPDCSLGTLEALMLKRQRSLDVPGARIPEIWFEYAKR
                     GSHPLMEAVLEHNAADVESLAAIAAECARTYANPVDARDCDRTALGTLWLKLQPDTGR
                     AVLERAFEEGEERAGWMLLRHYRRNGVWAEYRRVLSALSPGWDSWIERAKDAEHRLGD
                     PRAALQAARSARVFAADTELREHLELRIRRLTRKTAAIRQREDRTSRPR"
     gene            complement(23545..>25180)
                     /locus_tag="P5313_04850"
     CDS             complement(23545..>25180)
                     /locus_tag="P5313_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HRY79724.1"
                     /translation="LCSATIGNPGALAELLVEEPVETVTENGAPRAEKLVVFYNPPLV
                     DPVQGIRKSTATESQKLAVKLLRAGVKTILFARSRLRVELIAGYINQELRNLYTDNSG
                     IVVEPYRSGLLPTERRAIERGLRDGTVHGVVSTNALELGIDIGGLDAAIIAGFPGSFA
                     SFWQQSGRAGRRSGLSAAFFVASSSPLDQYIVAHPEYFFGASPESGRVDPANPYVYMD
                     HVKCAAFELPFGEGEPFGQDVGEALAYLAEDGIVRKAGGRWHWSSSGYPSEEISLRSA
                     ASDNVVIVDATEGRNRVIGEMDRPSAKELVYDDAVYIHRGRQYVVLKLDLENRLCKVE
                     EREVNYYTDAVVKTDIKVLSEDARGESAGHGWVLSDILVRSEAERFKKLRFHTHENVG
                     YGDIHLPPEEMQTRGLAVLFPPETSAGTELEKRDIAFRAAVLSGMSHLIRRIAPAFLM
                     CDPRDIGAAERARDDHFGVPALYLYDQYPGGTGLAEALRELLPKVFRAALETVELCPC
                     GGGCPSCIGVDFSDGRTALDGREVKTGVERLLAASA"
BASE COUNT         4504 a         8160 c         7992 g         4524 t
ORIGIN      
        1 cgggatagcc gcggttcgag gggacctgct cctgggtgat ggcgatctcc ttcatggcgt
       61 ccgagaagtt cgtcatgtcc gtcaggagca cgaggacgtt ccggcccttg agggcgaagc
      121 gctcggccac ggccagggag atatccggaa ccatgaggca ctccaccacg gggtccgagg
      181 cggtgtgtac gaacatgatg gtcttggaca gggcccctcc ctgctccagg gtgtccttga
      241 agaagaggta gtcgtcgtac ttgagcccca tgccccccag gatgatgagg tccacctcgg
      301 cctgcatggc gatccgggcc aggagctggt tgtagggctc cccggagacc gagaagatcg
      361 ggagcttctg gctcttcacc agggtgttga acaagtcgat catcgggatg cccgtccgga
      421 tcatctcccg ggggatgatg cggttggcgg gattgaccga gggtccgccg atggggatga
      481 ggttttccga gagctccggg cccttgtccc ggggttcccc ggcgccgttg aagatccggc
      541 ccatcaggga gtccgtgaag gacacctgca tgggatgccc gaggaagcgg acctcgtcgc
      601 ccgtggatat gccccggccc ccggcgaaga cctggaggga gacctcgtcg tccgccaggc
      661 ggatcacctc cgccaaggac acgccgaacc gggtgctcac ctgggcaagc tccccgtact
      721 gcacgtcctc ggcgcggacg gtgatcacgt tgccggtgat ggattcgatg cgggaatata
      781 ccttcttcat gtcacttctc cagggtctcg aggatgcggg ccgccttggg atccaggccg
      841 gtcttgcgct cgccgatcag ggtccggagg cccttctcga cctgggcgaa ccggtcgctc
      901 ttccattccg agccgttcag gtccaggaag ccctggcgca gcttgtagaa ccagtcccgg
      961 gcctcgtcct tgtccttgaa gcggaaggcc gtacccagca ccccgatgac catggagaag
     1021 acggacttct gccgctccgg ggagaccgcc gcgtccacgg gatcgaagga gttctgctgg
     1081 atgtagaccg cgtccaggta ctcgctcttg aggtacacga cgtagtcgtc cagggaggtc
     1141 ccctcctcgc cgacgacctt catcatctgc cccacctcgg ccccccgggc caggacggcg
     1201 tgggcgtagc ggacggcttc cgcggagagg atgcccgaat acttggacca ggactccagg
     1261 gggtggatgg ccgggtactt gcgggcgtcc gagcgctccc gggaaaggcc gtggaaggcc
     1321 ccgacgacct tgagggtggc ctgggtgacg ggctcctcga agttgccccc cgcgggggac
     1381 acggtcccgc cgatggtgac cgagccggta ttgccgtccg cgaggcgtac gatgcccgcg
     1441 cgctcgtaga aggaggcgat gacggattcc aggtaggccg ggaaggcctc ctcgccgggg
     1501 atctcctcca ggcggccgga catctcccgc atggcctggg cccagcggct ggtggaatcc
     1561 gccagcagaa ggacgtccag gcccatctgc cggtagtact ccgccatggt gaccgcggtg
     1621 tacacggaag cctcccggga ggccacgggc atggaggagg tgttgcagac gatgatggtc
     1681 cgttccatga gggagcggcc cgtgtgggga tccaggagct ccgggaactc cttgagggtt
     1741 tccacgacct cgccggcccg ctcgccgcag gccgcgacga tgacgatgtc cacgtccgcg
     1801 tggcggctgg tgatctgctg gaggaccgtt ttgccggccc cgaaggggcc cgggatgcag
     1861 taggtgccgc ccttggccac ggggaagaag gtgtcgatca agcggacgtg ggtgaccatc
     1921 ggctccacgg gcttgaggcg ctcggcgtag caggtgaccg cccgcttgac gggccactgg
     1981 aacatgaggg tcacggggtg gaccgtgccg tcctctccct ggagctccgc cacccgatcg
     2041 gtgaccttgt aggaccccgc gggcgccacg gacttgacgg tgtaggtccc caggaatccg
     2101 aagggaacca tgatgcggtg ggtgaaggct ccctcgggca cggtacccag gaggtccgcg
     2161 gcacggacct tgtcgccggg cttggcggcg ggggtgaacg cccaagctct gtccctcggg
     2221 agggcttcca ggtagacccc gggttccagg aagtggccga ccttggcggc cacttcgggc
     2281 agggggttct ggaggccgtc gtagatctgc cccaggaggc ccggcccgag ctccacggcg
     2341 agcatctcgc ccgtgaaggc cacgggatcc ccgacccgga tgcccttggt catctcgaag
     2401 acctgcacct ggaccaggtc cccccggatg cggatcacct cgctcttgag gcgcttgccc
     2461 cgggtctcga tataggcgac ctcgttcatg gagacggccc cttccacgcg gaccgtcacc
     2521 atgtttccgt tcacgcccgt cacgcttccg ttcgtcttcg tcatcgtatg tctccgatcg
     2581 ctctgaatct gtcctacgcc ccgcggcctt cggcgggggg cgcttctgcg gtatcgagga
     2641 tctcgtgata ggcggtccgg taggaggcct cgccgcgctc cgcctcgaac cgggccttgc
     2701 gctcgaggat ccggagcttc agggcatagg ccgccagggc ctccagatcg aagtagtgtc
     2761 ccgcctcgag gcgctccagg taggcccact tctcccgctc gatgataagt tcgccctcca
     2821 ggggatcctc gcactgggcg gccgcctggg cagcccggat gccgtcccac tccgggtccc
     2881 cggggcggag gaacttttcc ggagccctgc ccagcttgtg ggcccgcagt cgcgccagtt
     2941 cgttccggag ggccttgtcc caggtccgga aggaggacag cacccgggac gcgcggaccc
     3001 ggcggaccgt ctcggcatcc tcggcctccg tgtcgcccgc ggccgcgagc acgaggaaat
     3061 ccgcgtcccg catccggccc tcgcagcgat ccaggaagcc ccggaacgag aagggcggcg
     3121 ccgaggccgg caggagcgta gggagggtgg agcgcaggta gtagtacgcg ggcacggcgc
     3181 tattctttcc ggatggccgc cgcggcgatc tggcccagcc gggcgttcag gtactgggag
     3241 atcatctccg caagggagtc cgcggagaaa tcgtagtacg ccgccccgcc cttttccgcg
     3301 atccggaaac cggccttgat ccccgggaag gggcgtagtt ccaggccctt tttcagctcc
     3361 gactcaagct tcttccggaa ggacgcctcg agtttggaaa gctccgcggg cgggagcagg
     3421 acggccaggt cgtcggtgcc cttgtccttc caggcggcca agaccgcggg aatcgccgct
     3481 tccaggacgg gccccgagag ggccttttcg gtgtccgtcc gcacgatcgc ggcgagcacg
     3541 gcctcgatct cggtgcggaa agagatcacc aggttgcggg cggcctgttc cagggcggcc
     3601 ttgccggctt cctcgaagcg gtcggcgtcg gccttggcct tggccaggag ggccttcgcc
     3661 tcccgatccg cctcggccag gacggccctg cgccgctcct ccgcctccgc caggaccttg
     3721 gcggcgtcgg tctgggcggc ctcgatcccg tccttcctga tcttgtccag aagttcctga
     3781 agtcgaatgt ccatggcttc cccttatccg gattgaaatc tatggggcgt cttccgccat
     3841 aacgtaggag agcaccttgt ccatcttccg cctaagcagc ttgtggacgg cggcgggata
     3901 ctccttggaa gttatgaact cgcgggcgac ggcgtccagg tcctctacga tttcgtccac
     3961 acttttatag cgtgacgaat tcattccgtc aatgctggag atcagcgcgc ggaccgattc
     4021 ctcctcccgg gataggaggg cctcgttttc caggagcacg tagttcgtga tctccgagct
     4081 ggcgaaccgc tcgcggtaac ggccttctac ggacttgaga tcctccagga ggtcttccag
     4141 ctcggctcgg agtattttca ggaagtacaa acacttgcgc tccatgctcg cctccctcgg
     4201 gattccggat gatttcagta tcgtccatcg gggagggccg cgcaaggagc cttaggcccg
     4261 gctgaggaag agtgtgtacc ttacgccgaa cgatgtgggg ccgccgaagg cgcgtccccg
     4321 aaatttggaa aacctttcaa gaaaaatata tcttattgac tgcggtcatt tgaaaccacc
     4381 cgaaccacgg actcggaagg gtgcgcggac cgaaatccgc ctagaacgcc tccacctttc
     4441 gggccaggtc cacgggcgga tagatctgct ccgccaccgg gggcatggag ttgtacaccc
     4501 agtccacgcg gatgactccg ccccggcgac ggggctcgaa ccggccccgc accaggacgt
     4561 cctccatgtc cagggacatc gcgaagaggg ccgtcgcagc gccggcgggt atccgggcaa
     4621 gatccgcggg gaactcggtg ccgggcagca ggatactccg accgtccggg caggtcgcgg
     4681 ccagctgggg aacgatccgg ggaaagccgt cctcggccac gtaggagaga aatttcaggt
     4741 tcccggtctt gcggatgaag gcccgggtcc aggggttgat cggatccggg cgctcgagct
     4801 ctgtccccct gccgagctct gtccctcgaa tcctcccctc cccgaggctg ccgcgcaggg
     4861 ccccggcaag aacgcctcgt gcgaccgcgg ccaagggaag atcccggccc ggggtcacgg
     4921 agatgagatc caggtaatgg acggtatgga tgccgaagta ggagttgtac ctccacatgg
     4981 gtttccggtt catgagctcg tgctcggggc cgctttgggc cttgccggtc cacagagcgg
     5041 tacccttcca gagcctgcgg tccagggtca tgagcagaaa tccgaccttt ttccgctcct
     5101 ccgcgtaccg ttttccccgg ccttcgcaga attggccgaa gatgaggtgc cgctcgtcct
     5161 gggcctgcat cgcggtgacc agagtcacat ggggtaatcc ctcgggatcc agggtcgcca
     5221 gcaggccgac ctttccgtcg ggctcgaaga atttgaggtc ttcaagctcg aaatggtcgg
     5281 tcatccgttc cggtcgcgcg cgcatggagc ctcctcgttc caggatcttc gtccgattcc
     5341 cggctccggc gagccgagcc gctctttgcc cacggtcccg ccggccgccg tcatgcccgt
     5401 ccatcggatg cgatccgccg gccagcccgc ccggatcgcc gcgtccacca gccggtccat
     5461 gtgatcggca tccatcaggg gcgaggccgc cagctcccac tcctcgccca gctgccggta
     5521 tttctcgcgg cagaggttgt tgaaggcgca aagttcccag cgttccacgg attccgctcc
     5581 tgattccacc aggaaccggg cgatgccttc gatcatgccc ggagaatcgg ccaagccggg
     5641 gatgaccggg gtgcggaccc agatccgttt tcgagctctg tcccccgagg gacgcgcgag
     5701 caagcggaaa ttcctcagta ccacggccaa gtcgcccccc gtattccgca ggtgacgatc
     5761 cggatcggcg tccttgatgt cgtacaggac gaggtccgca tgctcggccg accgaagcaa
     5821 ccggaattcc gtgcacattc cgcaggtatc cagggcggtt ccgacccctt cgcttcggag
     5881 gcgccccagg gtttcctgta cgaacgacgc ctgaaggcag gcctcgcccc ccgaaaccgt
     5941 cacgcccccg tccgggccga aaaaggagcg gtcccgaagg agctcggcga cggcttcctc
     6001 cgcggtcatg gaccgccccc atagttccag cgcccccccg ggacaggtcc gctcgcactc
     6061 cccgcacccg tcacaggacc ctcgatcgat ccggatgccc gccccgaggg acagagctcg
     6121 gttcgggcag gcggacagac attccccgca gcccaggcac cgggacccgg accagcggag
     6181 ctcaggtttc ggcgagatac tctcggggtt gtggcaccag cggcagcgca gggagcaccc
     6241 cttcaaaaag agggtgctcc gcagccccgg tccgtcctcc gtgctcatgc gctggatttc
     6301 cagcaccagt cccctctcag atctgctcat gggcgatccg cgcgatcacc tcgtcctgga
     6361 gctcccggcc gatggaggtg aagtaggcgt tgtatccggt cacccgcacc aggaggtgcc
     6421 ggtattccga gggattccgt tgggcgtccc gtagaacctc ggggtccagc atgttcacct
     6481 ggagggccgt tcccccgttc tcggcatagc cccgaaggaa actcttgaac ttggcccggt
     6541 tctcgggccg cttgatcagg gaggggttga agctgatggt atggctggcc ccgttgggca
     6601 gggaattgaa gtacccgccc cagtctccgc cctcacccct cccgccgagg accgtcccga
     6661 cggagttggc gttcgccgtc ggcccccgga tgtccgcccc attggacggg cagatggcgt
     6721 tggaaaggaa ctggtccttg ggccgcccgt ccggggaggc cgggaggatg aacccgtccc
     6781 cgacccagta gttccaggag agcatcccgg gccggaaccg ccgcccggtg gattcggtac
     6841 ggtacttcca ggtctccccc gtccagaggt ccatgacccg gcgggcgagc tcgtccgcct
     6901 ccggatcgtc gcgtccgtac ttcggggccc tgttccgggc ccgggcctga aggatctcgt
     6961 accccttcca attgtccttg agggaccgga tcagctcgtc catcgtgcag aaacggttgt
     7021 cgtacaccag gtacttgacc gcgagcaggg agtccacggt cgtagcgaag gtgaccccct
     7081 cgatggtgac gtacccaagc tccgcgcccc cccgggtcac gtcctttccc ttttcggcgc
     7141 agccccggac gagggtggaa aggtagggag tgggatggaa ccgggcccgc aggctttccc
     7201 cgcgctcgta aagtccggtt atctcgcgga taagggcccg ggtctgctcg gcgtaggcgt
     7261 cccagaaccg gtcgaagtcc ccgaatcggc gggggtcccc ggtggagggg ccctgtcgac
     7321 ggggccgttc cgtgacaccg gtgatcgcgt ccgtatgcgg cacgagggga tacccgtttc
     7381 ccagggtcag ctccacggcc ttgaggaggt tcaggttgca gtccaccgtc ccggaccggt
     7441 cgttcccgac catggtgttc tccaggcagc ccaccgaggc gtactcgtga acgttcccct
     7501 cgtggatccg gtcttccgcc ccggacatcc gggcctgccg catcatgccc gccatggacc
     7561 tctcgtcgaa gttgaggagg aagggggcgc cctggctgga ggcgatcatg tccacgacct
     7621 tgtccaggag ggcctcgggg ctgccgcgat ggagccggac attgggtttg ggttcgagga
     7681 tcggggacat ttcgtcgatg acctccagga tcaggaaggt gaggtcgttc gtcatgtccc
     7741 ggccccccgg tcccaggccc gagaggttga agagttggcc gaaccctgcg gtgatgccct
     7801 ggtttccgac cttgatcatg gagtcgtagg cggtgttcgc gtggatccag aagcacttga
     7861 ggatttcctt ggcgaactcc cggtccatgc cctcccggac cgaggcttcc cagtagggca
     7921 gaagcatttg gtcgagccgg ccgaaggaca cgccgggccc cggatagttc tcgtcgctca
     7981 tcaccagcat atgggccagc cacagggcct ggacggcctc ccagaagtcc cgagcgccct
     8041 tcgccggcac ccgctcgagg ttggcggcca tgcgccggag ctccgccgcc cgagccggat
     8101 cccgctcgac atccgcgagc ctccgggctt catcccggta ctttccggcc agctcgaccg
     8161 gcatcccgca ggcggtgatc atggcccgga gctgggctcc ccgggggccc tcacggtcct
     8221 cgggggacag agctgcgaga ttcccctcga gctccgccag gattccctcg aatcccccgg
     8281 ccaggacccg gccataatcg gggatgaggt gcccgcccgt gggccccgcg ttcccgaagc
     8341 cgtggttgtg gaacccgatg gcggccttcc gaagcccgct cgggccgtag agcaccgcgg
     8401 aacgctcttc atgctccctc ttggtcaggc acagcgaggt atgtacgttg aagcgtgccc
     8461 cggcgatcag gtccctcgga aggatctcct ggggcaagta ccgggtcatc acctccttgt
     8521 tgaaccaggc ccggcgctcc ggaaggctga ggttccagaa atccggggga agcgccacgg
     8581 gccgtgcgat ctgacggaag gagctcgtga aggtctggaa gaaggcgtag gtctccggaa
     8641 cgatgtggta gttgcactcg ttgtactgga tgtcccaggc tgtgccggtg gtgaaactga
     8701 tctgttggtt gttccaggcc cgcgatgtcc cccggaagta gtagtcccgg agccatcgga
     8761 tccgaggcga aaggcccgcg ggctgcttaa tcgcggacgg ggaatccgtg gtcttcatgg
     8821 ggaacctcct tcaagttcga tcgcgccgtg gagatcgcgg gggcattcga atccagggac
     8881 agagctcgat agacccgatc gcgtccgtag gccagggcga gggacagagc cgcggcggcc
     8941 ccggattcgt cccgggcttc caccctgtcc aggaagcatt ccagttggga gaaggtggcc
     9001 gaggcgcttt cgggttcggc gtagaaacac cgggcatagt aaaggtagac ggaacggaaa
     9061 gcgtgaaaca gaagctgcag tgccagattg tccgtctgct ccacgaagag cgcatgaaac
     9121 tcgtaatcct tcgccgctaa tccctccgga tcgccttcct cggcgcggag ggccgccaag
     9181 gccgagcgaa gggcccgcac ccgttccgga tcggagcgtg ccgccgcccg ggacgcgcag
     9241 cccgactcca ggatctcccg cagttcgttg atcgcccgga aaagctccgg atccaggggt
     9301 tcgttgttgt ccaacaggta gaggaggaaa tccagggagg cttccttccg gtaatccgta
     9361 acgtaggttc cgctctgggc ctccgtgcgg acaaggccca tgctggaaag atgtcggatg
     9421 ccttcccgta gtatcgagcg gctcacccca taccggaggg acagagctcg ctccgcggga
     9481 aggcgatccc cgggcttgaa cgtgcctgcc aggatctccc gttccagttt tttggtcgcg
     9541 atgttcaact tcttgcgcat attggtccta ccagttagaa tatcatggca tccaggtccg
     9601 cgcaagcgta tagtaacagg gacagagctg attcgaagcg gcctgcccga tacctcgaaa
     9661 caaggagcga ccatgacgaa cggccttccc tggcttatga tcctgccctc cggaatcgtc
     9721 ctgggcttcc ttcttcattg gctaacgatc cggggcatcg ataccgcgga aggacgcctg
     9781 ccccactgca ggggtgccta cctggccttc tcggcccagc tcctgacctt cgcctgggcg
     9841 gtctacttca cggccggcct cgggcgatac ggagccgtcc tcctccccct gggcatgacc
     9901 tgctccttcg cgggggatta cttcaacctt cagttcccgg caattcggaa ccgggttcgg
     9961 gagccggtct tcctgggcat cctcagcttc gcggccgccc aggttatcta tataggtgcc
    10021 ttcctgtcct tgatcccctt ccgggagctt gcggagcgag ggtactttgt ccccatttta
    10081 gcggtgctcc tgatcgcgcc ggcgatgata ttccggctga gggtctacca acccggacgt
    10141 ccgaggagct tgatgctggg aggattcctg tacggattcg ccctgggaac catgacggcg
    10201 atcgcccttt ccgcggccgt gacccgggga gggtcctgga tcccggtcgc cgcgggagcc
    10261 ctgttcttcc ttctttccga cgccgtcatg ggcgagacga ccctccacgg gaggcacccg
    10321 aagcacgagt accaggtacc ctggggaacc tatctcgccg cccagggtct catcctggcc
    10381 gggacggcct cgatcctgat ggctccgggg tgagtccgtc ccggacccgg ctcgatcctg
    10441 cctcgcgctc ctgcgggatt cacagaagct tcatcttcat ccagcccgag gtgttgtgga
    10501 aatccgcgac cgcgctcccg ggaggcacga gggaatccag ggccgccgcg gtctcggcgt
    10561 ccaggctcag gttgagaacg ggcagggctt cctcgagctg ggcttccgta cggctgccga
    10621 gcagggccgc cgctatgccc ggctggtcct tcacccagag gagggccagc tgggagggtg
    10681 tcagcccccg atcggccgcc aatccggcca gggagcgggc gaccttgatc ccccgctcgg
    10741 tgagcctagc ggcgacatag gaaacccggg cggccttgga gtccgcgggc ggccgggcgg
    10801 cgtcggcgta ccgccccgcc agctggcccg cggccagggg cgaccacgcc aggatgccca
    10861 cgccgtgccg gagggcaagg ggtaccagtt cgttctcgat ccgccggtcc agaagattgt
    10921 aggggggctg ttccgagacg aaccgcggaa gacccagccg ctcgctgatc cccagggcct
    10981 ccatgatcat ccaggccggg aaggtcgagg agcccacgta ccgtaccttt ccctgacgta
    11041 cgaggtcgtc caaggcgcgg agggtttcct cctggggaac cccgaaatcc gggcgatgag
    11101 cctgatagag atcgatccaa tccgtgccga ggcggcgcag ggacgcctct acggcgcgga
    11161 ggatgtggcg gcgggagttc ccccggtcgt tcggtccatc cgacacgggg aagtggaatt
    11221 tcgtcgccag gacgaccttg tcccggagcc ctccccccag ggcccggccc aggatccgct
    11281 cgctttcccc cgccgcgtag gagtccgcgg tgtcgatgca ggtgatgccc gcgtccaggg
    11341 cgcggtggat catgcggatc gcctcggcct cgggggtcgg atccccgaag ttccaggtgc
    11401 ccagccaaag aggggaaact cgtagccccg aacgccccag gtatcgatat tccattcaac
    11461 acctccgggt cacggggcct ttcgggccgt gaccaacatt tcgaagtcct ccgtcgtcaa
    11521 ccggtggtcc cgggaaaaag cccccaggtg cgccccgaag atcccgatgt ccgtgaatcc
    11581 cgcagtcttg aggagccagg tgatctcgct ggggacgtag taccgctcgt cgcactccag
    11641 ggtcttggta ttgccgagat cgtccaccac ctcggtcacg ttgtggtccc ggaaggtcat
    11701 gaggtcgaag gtattgttcc ggtagacggc gttgccgggc tcggaattgg acgagcagaa
    11761 cacctccacc gagtggacga gcggaaaaag tccgttcaag gtcgtgaaga tgaggatgcc
    11821 gccgggtttc agggcccggg cggctccgcg gaggatctcg aagttcatct cgtccgtctc
    11881 catgaggggg aaggccccct cgcagagcat gatggccgcg tcgaactcgg cctcgaaggg
    11941 aaggttccgg gcgtcctccc gccggaactc cacggacagg ccccgctccc gggccttatc
    12001 ccgggcccgc tccagctggg actcggacag gtcgacccct atgaccgcgt agccccgggc
    12061 ggtcagttcc aaggtatggc ggcccgtccc gcatccgatg tccaggatgc gcttcgagcg
    12121 gtcgctcccc agctccttct cgatgaagtc gcattccccc gaagtccccc gggtgaagtt
    12181 ctcggcatcg tacttccggc cgtagttctc gaacagggct tcgtaccagg ctttcatgag
    12241 acctccagac gttcatgata tcagaaatgc ggggacagag ctcgagcaac ctggggacag
    12301 agcctggttt tttacaacaa ccagggacag agcttggata tgctccttcg gggagctcag
    12361 gggacagaga agcgacccac cccccgaggg acagagcatt ccatagccgc gaggctttcc
    12421 acggaaagca ccgtcgcccc ccggtcctcc tgtacgcggc cccggaccca aagcggcttg
    12481 ccgtcgtgga ggaggaatcg gtagcgcaga tatgcatccg ggaacagcac ggcttcatac
    12541 agggacgtct catcttcgaa ggatacgaat tccatgggct cgtcggcgga ggtcagcaca
    12601 ggcttggccg ttatcggcca gcccgcgagt tcaacccgtc ggcccgccaa atccggcaga
    12661 tcctttccaa gagtccggga acgcttgagt gcctgggagc aaagcgtaag cgggtggacg
    12721 tcaagggtgg ttccgagata tcgcatctgg gcttcgagcc gcttgtgctc gggcattggg
    12781 acgtgccctc gccggtagtt gagctctgtc cccggggtgg gttcaccgaa gagctccgtc
    12841 ccccgggaac aaggttcttc tctcgccgcg gcccgggaga gcagacggag cagcttctgc
    12901 gaacagctct gtccctcgtc caggaagtcc aaggctcccg atcctgcaag ggcttcggct
    12961 tcggtgcgat cgatcgacac gcggcaaccc aggtcgtcca cgcttcgata cggcccgttg
    13021 ctcttccgtt ccgccacgat gccggcggcc gtctccgagc cgagactcga gatcattccc
    13081 aatccgaaac gtatagtccc gtcccggccc cgacagcgga tttcgctctc gttcacgtcg
    13141 ggagccagaa tgcgcaaccc catccgcctc gcctcgctgg tataggccag ggtcgagtag
    13201 aagcctcctt tgttggacag gacggcggcc atgaactccg caccgtggtg agcacgcaga
    13261 tacgcggagc ggaacgacag cttcgcgtag ctcgcggagt gggctttcac gaacgagtac
    13321 cccgcgaagg actcgatcat ctcccaaacc gcgacgatcg tcttgtcgtc cacgccgcgg
    13381 gcccgggagc cggacatgaa ctgagcccgg tatgccgcta atcgagtccg gacatccttc
    13441 ttggacaaga ccttgcggat tccgtccgcc tcggccggag tgaatcccgc cagggccacc
    13501 gccgccttgc tcacgtcctc ctgatagcag aggatgccgt aggtctctgc aagctggtcg
    13561 tccagcaagg gatgaagcgg ctcgtaggtc ttgccgttaa gccggtcgat gtattcgttg
    13621 atgtaacggt tggccgcggg tcggatgatc gacgagcgga tgacgaggtg ctcgaaatcg
    13681 cccttgcccg tcttcttctg gaggagccgc atggccggac tttccacgta gaacaccccc
    13741 agggtgtcgc cgcgggacag gagatcgatg gtatccgcgt ccgaaagagg attccagtcc
    13801 tcttcggcga tcggtgtgcc gttttccgcc aggttggcca gggcgtcccg aaccacggcc
    13861 aggcttcggt tgccgagaag gtctatcttg accaaaccgg ccgcttccac gccttcctta
    13921 tcccaggccg tgacccggat gccggaaccg ctgggaacca cggggacaag atccgaaagc
    13981 ctgcccggta ccagaaccag accgccggaa tgaaccccca ggtggcgggg aaagcccgcg
    14041 atccgcgggg ccagttccag gatctccttc cagaccggat ccgcctcggc cagggtacga
    14101 ccccggtcca ggtaaagggc ccgctccacg gctcctatct cgccgtccgg catcccgaat
    14161 gcccgtgccg tctcccggag ggcggaacgg gcctgaaaac agacgtggtt cgagacccgg
    14221 gcggcgcggt cctcgccgta ctcccggatc acggattcca ggatcgcgtc cctttcgtcc
    14281 caggcgaagt ccacgtcgat gtccggcggg tctttccggc cggggttcag gaagcgctcg
    14341 aaatagaggc cgtgggccag gggatcgata tccgtgattc ccagagcgta ggaaaccacg
    14401 ctggcggcgg cgctgccgcg cccgcaggtc cgggagctct tccggactat gtcccgcacg
    14461 atcaggaaat aggcggaaaa tcccttggat tcgatgatcc ccagctcgta gtccagccga
    14521 gatcggacga tgccgtcgat atccccgtat cgcttcgccg cgccttgata gaccaaggat
    14581 cgcaataccg ccgaagctct gtccccatcg caggacttgt attcgggaaa gacatacccg
    14641 tcgaagagcg agcatagctc gatcgactcc actacggctt catttgtccg tatagcctcc
    14701 ggcacgctct ggtagggccg gattgcgtcg agcggagacg agaggattgt gcccggggcc
    14761 gaaagctccc cttcctggac ctcgaatgtt gtcctgccgc ttcctatcgc cacgagaagc
    14821 ctctggactt ccctatccga cgggcgcagg aaccgtactt ctcccgtcgc aaggaggggg
    14881 gcgcctgtcc cggaaagaga cctccaggag agacgtgccc ccttgacagc ccggctgacc
    14941 ggcggggcaa gaagggcata agcccgtcct cgggctgcct cgagcaaact catgtcgtcg
    15001 gtcgctatca ggagatccgg aggagggcct ttgcctagaa tacagggaca gagctcaacc
    15061 tccgttcgaa tcgccgcagc cagatcgaaa tcacctgaag ccctgttcgt gaggattcgg
    15121 catatccggg accaaccgga acgtgtccga gcgatcagga gcgtccggac atgcccttcc
    15181 acaagctctg tccccaggat cggggaaatc tccgcatggc cgtcgagctc tgtccctcgc
    15241 tggactcgag gaccttccgc gcaggcctcc aagaacgccg ggaccccgta tactccgttc
    15301 cgatccgtca gggccagagc ccggtacccg aggttacggg cctctcgcac aagatcccgg
    15361 gggaacatca cgccgtagtg aaaggaataa caggatgaaa ccagcaaggg agtgaacatt
    15421 gcctgtactt aaccgacggc gagaccgtga agatcatccc cggcttcgcc cgggaccccc
    15481 tgacagagaa ccagtgagga aatcggtaca gaactgcgat cgacggcccc tccgttcgtg
    15541 ccggtccggc cggccgccag gatgatcgcc ggggatacgg cggaaaaacc gtatcgggtc
    15601 cgcaccctgt ccagagccgg ctgcaggcgg gccaggcggg tttcctcggg ctcgaacaga
    15661 tcgagctcgg gaccggcgga gcccaatccg gaaaactcga tggatatccg gcggacccgg
    15721 acacggcggg ttcgcgcccg gcgaagcgcg tcccggaccc cgtggatgac ctcgtcgtcc
    15781 cggactagaa cgcggggaga tctagcggta gcggcatttc ggagaccgtc agcgtaccat
    15841 acctctaccc tgatgttccg cgctcccatc ccctttctcc ggatggagta cgcaagctcc
    15901 gatgccagca tgtctaccct cgggttcagg agttcagcat ccacgacatc cggctcgaaa
    15961 atgaacgcac ccctctccgt acggcgttcc ggaggctcgg gatccacggg gacggcgtcc
    16021 atgccccgag cccgagccgt caactccggt ccccggggac ccagggctcg ggcttccgcg
    16081 tccgacagcc cagccaagtc tccgatctct ccaatgccca ggaggtccag gcgccgggaa
    16141 agggcggccc ccacgcccgg aaggaactcg acgggttgat ggcgcataag ctggttttcc
    16201 tcccaggggg acagagctat gaatcccgca ggacgaagga ctcgcgtggc caccttgctg
    16261 acggtcttgg atcccgcaag ggcaagaacc ggacggagcc cggttgcgga aaagatatcc
    16321 ttccggatcc tctgggatac gtcctccggg gggccgttga gccccgacgt tcccgccaga
    16381 tccgcgaaga cgtgcccgtt gcccgccatc tccacctggg ggctatagga acgacagatc
    16441 ctgtcgagct cgccgttcgc ccgggcacag atatcggtgc ggggttctcg cacgataaga
    16501 ccggacagca tcgcgaccgc ccgggaaaga agcatgcctc gcagcacccc ctctcggtgt
    16561 gcttccctcg aaaggtccag aaccacggca cgaggcgccg ccctgttcgc gaccacgaaa
    16621 gggcgtcccc gaagggtcga atcgaggttt tcttccaggg cggccatcaa cccgatgaca
    16681 ttgacgtgca ggacggttcc cgtacccatg gcggccccct agagaaggcc ggaggaggtt
    16741 gcgaggcagg cgaaatcgcg ggcgttggag gccgcgttcc ccctcctgtg gttattgaag
    16801 tagacccgaa tcttccgggc ctgggaaccg agggaaccga gtcgaggggc ccacgcggat
    16861 aattcctccg gactgtaaaa gtactcgtag cgggtgcccg catctcccgt ccaccaggcg
    16921 aaggcgttcc gcccatggaa tcggacatag gcgagatccg ccgtgacaag gtccaagaca
    16981 ggaggaaggc ccggcaggcg gggggcatcg aggatgcaaa ggcccacatt ccgtgctcgc
    17041 aaggcctcga taacccgagc gctgtaccac tcggcattac gcagctcgac aaccagcgga
    17101 attccctcga gttcgtccag caggcgagcg aggtacaccc gttcgccggc ggaataatgg
    17161 aaagacgagg gaaactcaag caggaccgcc gcgagccggc cggcccggat cagaggatcg
    17221 attcccgccc gaaactcgca ggcggtggcg ggaagcgtgc gggcatcccg ttcatgggtg
    17281 aggttccggt aggccttcac cgcgaaatcg ataggcctcc ggaggcgtcc aagcattcgg
    17341 tcgaagatct ccgctcgcgg ctggccataa tacgtgaagt tgatttcaag ggtgtcgaac
    17401 tgctcgctat agaaccctag aaaatccttg cgggcaagcc ccgggggata aaagcttccc
    17461 ctccactccg gatagtcata tcccgaggta cctattcgaa tgtcgagatc ggacggcaag
    17521 gagtccactc ttacgatata ctacacatat gtgtagtttt caagtgcaaa actccctttc
    17581 ccctagggac tcctatcggg cagccgaagc agctcatgcc ggggcgccca tagcagagcc
    17641 tcgcagggag gtttgacaag aacgtgcctg tatcccaggc gatcatgcca tagaccgccg
    17701 tgcttccatt cccagtcatg gagcaccaga cccgcattta ccgggtttcg atcaatatag
    17761 cgaaagatct ccaagaactc gcggaacccc tctatgatct ttgaaaaaaa gcgttcgccc
    17821 cagacatgcc cggaataccc atgtgcccgg ttataggtca tcgcgaatac gctcatgatc
    17881 cactgcatga tccgtgacaa cgattcccgg ggccccggac ggatcataag gtggtaatga
    17941 ttgcccataa cgcagaaatt ctcgattcgg aagcagtatc tcttccgagc gcgccggaca
    18001 acctgaagga acacatcttt catgccttcc tcatccagga tgtgttcctt gcggtttgcc
    18061 cgagcggaga catggtagag ggcgctttcc tttagcaacc gtggctttct catacctctt
    18121 atccgtccgg gattcttaat ttcgcttgca tcgatcaagc tctgtccctt gaagcttcca
    18181 cagaaacttt cggtgttcat gcgcctctcg ctcgctagct cacagaggtt cttcggatcg
    18241 actcctttcc gtccaggaaa caagaaggcc cgccataggc gggccttctt gaatagcgcc
    18301 gaaagggagt cgaaccctcg tctccagctt ggaaggctgg ggtaatagcc gttatacgac
    18361 cggcgcgatt gtcgtgcgcg gattatgccg cgcgggaaac atggttgccg tccgttcatc
    18421 tcgcgccctt cggacgctcg gcgtacgacc ggcgcaactc gttatacgca caagaaaata
    18481 ccgggaaacc ccgaggtctg tcaaggcgga tccaagggaa gccagatcac gccatagggt
    18541 ggcagatcaa ggtaggctga accgcaaggc caggaaatta cttctcctgt gatcgggtcg
    18601 tagggccttc ccggaagacc taggtttgtc ccggtttcga accgggcggg tcgcgggctc
    18661 acatttactg cgcaaaggat acgcttctcc ggcccgcccc tgaggatgga gaacacgggg
    18721 ccgtcccggg atagtacccg ggacggcgcc cccggggcca gagccggttc ccggccccgg
    18781 caactgtaca gcgagcggaa gccggacagg caccgggaag gccggcttcc ggggtccatc
    18841 aaggttgact cgacggtatt caagtccaga acccgacggt tgatcgccct gttgtacccc
    18901 cggacaaccg ggccttcagc ccaattcggc gtgcccagga ggctatggat gtacactgcg
    18961 ggcatgccgt ccagggacgc agcgatcgca tggctggcta gatgagcctg gatccgcagg
    19021 tcctccggca gatcgggatc cgctatcgcg tcgaaccagg aagcgttcaa ctcgtatgga
    19081 accggccctt ccggtgtcgc cttgtaggaa atcaggcatc ctcgcccaag ctccttgtcc
    19141 aaggtctgtc ggaaagccgc ctcgtccaca agtccccggg cgggtgtaag tccgataccg
    19201 tcatgggaag cgagaaaatt aagaaatgcg cagccggaag gagcgcggag gtcctgagcc
    19261 caggcggaaa gatgacttgc gtccccggtt gcgaaggcat ggagggtcag ggggggcaag
    19321 gcgaaattgt atacaaggtg ggcttcatct ccggacccga agtacgcgac attttccgga
    19381 tgaggcacgt tggtctccgt gagcaggcgg acgtccaatt cgagggcgtc cacgacggcc
    19441 cggaagagtt ttaccacagc gtgggtccgg gggtgatgga ggcagggatg gccatcctcc
    19501 ttccaaaggt aggcgatggc atccagtcgc acgatccggg cacccttttc cacatagtcc
    19561 aggaaaacgt ccaacatttc cgcgagcacc tcgggatcgg agaaatccag gtcgacctgg
    19621 tcctcgctga aggtcgtcca gacatgtacc ctggagccgt cggtccgaac gtagggtgtc
    19681 aggaggggat gggtacgtgg acgcaccacc cgtgatgcgt cgtatccttt cggccgagtg
    19741 aggaaccatc cttcatactt cggatccccc ttcaggaacc cctggaacca aatcccttga
    19801 gaactcgcat ggttgatcac gaggtcgaag accagccgga agttcttccc cagcgcgccg
    19861 acctcctccc acccccccag atccggatcg accgctctgt aatccatcac ggagaagccg
    19921 tcatccgagg agaaggggaa aaagggcagg atgtgaatat aggagatcac gccggagagc
    19981 cgtctttccg cgaaacgcct cagcgcggcc aggggaacct cacccgttcg cccttccagg
    20041 gtatttccat acgttatgag gaatacatct ccctccgaga gtagtgtcgg ttccgatccc
    20101 ttgcgatggg cgtctgtatt ccggagtccg tccctgtgcc gcgccagcac ctgatcgatc
    20161 aataccttcg cctccgggtc cttcccggta tcgcgatata ggaaactgat ctgtttttcc
    20221 atttcgccct tcgcatccat aggccatcct tggagaacca gatggttcta tcctaatgcg
    20281 tttcctgctc ccatggaaat cattattgac tatatattgt tttatatata ttataagagt
    20341 cgacggcgga ggcaaatatg gagcaaatca caaacggcaa aaaaatcctg attatcggag
    20401 gagtggccgc gggagcaacc gccgcggccc gagcccggag gctggacgaa accgccgaga
    20461 ttacaatcgt ggagaaggga cggtacgtct ccttcgcgaa ctgcgggctc ccttacttca
    20521 tctcccggga catcccaaaa cgctccgccc tcatcctgca gaccccggag ggctttttct
    20581 cgagataccg agtcacggtc ttgcttgaaa cggaagctct gtccctcgat cgagcagccc
    20641 ggaaggtccg agtccgaggc cccgaggggg agtcggacct tacgtacgac tccctcatcc
    20701 tggctcaggg tggtacgccg gtcttcccgt ccatccgtgg gcttgactcc ccgaacgtat
    20761 tcaaactctg gacgattccc gacatggacg cggtccacaa gtttgtagag accgaaaagc
    20821 cttccagcgc agtcgtcatc ggcggaggat tcatcggcct cgaagccgcg gaagccttct
    20881 tgaaacgcgg actggctact tccatcgtcg aattgaccga gaccctgatg cccccggcgg
    20941 accccgagtt tggaatgttg atccggagat cctacgaggc tgcgggtgcc cgggtcttca
    21001 cggggcgttc cgtccggtcc gtggatgttt cctcgaggac ggtccttctg gatgatggat
    21061 cggaagtccc ggcaggaatc gtcctggtat ccgcgggggt ccggccgaat acggagctgg
    21121 ccaaagccgc gggactggaa atcggttcct ccggcggggt atccgtggac gaggggctcc
    21181 gaaccggcgc tccccacatc tggccgccgg ggatatggtt gaaatcgccc accgggtcgg
    21241 cggccggcgc gtgaggatcc ccctggcggg tcccgccaac cggcaaggcc gtatcgccgc
    21301 tacgaacgcc ctggggggtt ccctgaccta tcgcggcgcc ctcgggtcca gcgtcttcaa
    21361 gtccatggag gagaccttcg ccatgacggg cttgtccgaa aaggcagcgt ccgccgcggg
    21421 gtttgatgtc ggcacctcga cggtgtccaa ggcccaccac gtcacctact accccggcta
    21481 cgaggacctc ttcctgaagt tagtcttcga ccgtgccgac ggtcggcttc tgggcggcca
    21541 ggcctacgga cgggaagggg cggacaagcg catcgacgcc ctggcgacgg ccctggcggg
    21601 cgggctgacc gtggacgatt tggccgaact ggatcttgcc tatgcccccc cctacaactc
    21661 tgccaacgac cctatcaaca tggccgccta cgccgcccag aacgacctgt ccgggtactc
    21721 gcccgcacgg tccgcccggc aggccgcgga tgctctgggc cagggagagg ttttattggt
    21781 ggatgtgcgt acgtacgggg aatacgcgaa gggttccgtc caggcttccc tgcaccttcc
    21841 cgtggacgaa ctccgcgaca ggctggatga acttcccaag gaccgggaca tactgttgct
    21901 gtccaaggcc gggttcgaga gccacatagc ccttcggcaa cttagacagc ggggattcga
    21961 gcgggtttgg aacatttccg gaggaatggc agctctgtcc ctccaaccgg ggctccggtg
    22021 ggccgaatga acgacaaacg agaaacgcag aaaaggagat gaccatggcc aacatcgttc
    22081 aagacaagat cgcgtcgggt gccttcattc tcgacgtacg gaccctcgag gaattcgacg
    22141 acgagcacta ccccaacgcg atttgcatac ccgtcaacga actcccgtac cgcctctccg
    22201 agatccccaa ggacaggagc gtgatcgtgt actgcgcttc gggcgcccgg agcgcctatg
    22261 ccgcgcgcat cctcaaggca tcggggtatc ccgatgtggt caacgcgggc ggcttgtacg
    22321 atcttcccgc tgccgtatag cggccgtctt tcgtgtcagt ctccggatac ggagctcaag
    22381 gtgttccctg agctccgtat ccgcggcgaa aacccgggcg ctccgggcgg cctgcagggc
    22441 ggcccgaggg tcgccgagcc tgtgctcggc atccttggct cgttcgatcc aggaatccca
    22501 acccggggac agagctgaaa gaactcggcg gtactccgcc caaaccccgt tcctgcgata
    22561 gtgtcgcaac aacatccatc ccgctcgttc ttccccttct tcgaaggccc gctcgagcac
    22621 ggctcggccc gtatccggct ggagttttag ccagagagtc cccagggccg ttcggtcgca
    22681 atcccgggca tctacgggat tcgcgtacgt ccgggcgcat tccgctgcaa tcgccgccag
    22741 ggactccacg tcggcggcgt tgtgctccag aacggcttcc atcaagggat gggatccccg
    22801 tttcgcgtat tcaaaccaga tttccgggat gcgggcgccg ggaacgtcca gggaacgctg
    22861 cctcttcagc atcagggctt ccagggtccc gagggagcag tccgggaacc gcttccgcca
    22921 gagtctccga gccgccggaa gaaaatccgc atggggatgc ggaggccagt gcttgcggtt
    22981 caggatgcag cgggtgcgaa gaaggggctc gtcgaaggaa cgaccgttat acgaggccag
    23041 caccgtttcc gagacgagtg cgcccaatag ggcatccagg aaggcgtcct cgccggggta
    23101 gtcgatgagc aggtactggc ggatctcgaa gccttcggcc tctcctccgg ggacgaccct
    23161 cccgagcccc gccaggaagg cgatcgttcc cgcccctccg gataggcccg tggtctcgag
    23221 gtccaggaaa agcaggcggc ccgccgggat gcggcaggac tccggagtgg tcgccaagcg
    23281 gcccggctcg ttatcgtccg ggacgcgtat cgccgccctt gatagatacg gccgaaggtc
    23341 gagggtttcc gggagcggat tgccgcgacg aatcgtacgg gtcaggacga agggtgcaaa
    23401 ttgactccaa ccggagagtt gtgcggagag tgacgccccc gtcgggccgg agacctcctt
    23461 cctctcgtcc gcccaaggtt cccgggccgc ctgggcgagc tcccgcgccc gggcgagccg
    23521 ggatctcagg gttcctgcca tttcctaggc ggacgccgcc aggagccgtt cgaccccggt
    23581 cttgacttcc cgtccgtcca gggctgtccg tccgtccgaa aagtccaccc cgatgcagga
    23641 agggcagccg cccccgcagg gacagagctc gaccgtctcc agggccgccc ggaacacctt
    23701 cgggagcagc tcccggagcg cctccgcgag ccccgtcccc cccgggtatt ggtcatagag
    23761 gtacaaagcg ggaaccccga aatggtcatc ccgggcccgc tcggcggccc cgatgtccct
    23821 gggatcgcac atcaagaaag ccggggcgat cctgcggatc aggtgggaca tgccggacag
    23881 cacggccgcc cggaaggcga tgtccctctt ttcaagctct gtccccgcgg aggtctccgg
    23941 cggaaagagc acggcgaggc ccctggtctg catctcctcc ggagggagat gtatgtcccc
    24001 gtatcccacg ttttcgtggg tatggaatcg gagtttcttg aacctctccg cctccgaccg
    24061 gaccaggatg tccgacagga cccatccgtg gccggccgac tcgccccggg catcctcgga
    24121 aagcaccttg atgtccgtct tgacgaccgc gtccgtgtag tagttgacct cccgttcctc
    24181 caccttgcac aggcggttct ccaggtccag cttgaggacg acatactggc gcccccggtg
    24241 tatgtacacg gcgtcgtcgt agacgagctc cttggcgctg ggccggtcca tctcgccgat
    24301 cacccggttc cttccctccg tggcgtccac gatcaccacg ttgtccgagg ccgcggagcg
    24361 cagggagatc tcttcgctgg gatagcccga ggaggaccag tgccagcgac cgccggcctt
    24421 gcggacgatg ccgtcctcgg ccaggtaggc cagggcttcc ccgacgtcct gcccgaaggg
    24481 ctcgccctcc ccgaagggaa gctcgaaggc ggcgcatttc acgtgatcca tgtagacgta
    24541 ggggttcgcg ggatccacgc gtcccgattc cggggatgcc ccgaagaaat attccgggtg
    24601 ggctacgatg tactggtcca gggggctgga ggaggcgacg aagaaggccg cggaaaggcc
    24661 ggacctgcgc cccgcccgcc cggactgctg ccagaaactg gcgaagctcc cggggaatcc
    24721 cgcgatgatc gcggcgtcca gccctcctat atcgatcccg agctccaggg cgttcgtaga
    24781 gaccaccccg tgcacggtac cgtcccgaag cccccgttca atggccctgc gctccgtggg
    24841 caggagcccc gaccggtagg gttccacgac gatcccggaa ttgtcggtat agaggttccg
    24901 gagctcctgg ttgatgtacc cggcgatcag ctccacccgg agccgggacc gggcgaagag
    24961 tatggtcttg accccggcgc ggaggagctt gacggccagc ttctgggatt ccgtggccgt
    25021 gctcttccgg atcccctgta cgggatccac cagaggcgga ttgtagaaca ccacgagctt
    25081 ttccgcccga ggggcgccgt tctcggtgac cgtctcgacg ggctcctcga ccagaagttc
    25141 cgcgagcgcc ccggggttcc ctatggtcgc ggagcagagg
//
LOCUS       DAOTEI010000032        25130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1290_length_25130_cov_5.405344, whole genome shotgun sequence.
ACCESSION   DAOTEI010000032 DAOTEI010000000
VERSION     DAOTEI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 25130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25130
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_25130_cov_5.405344"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3526)
                     /gene="dnaE"
                     /locus_tag="P5313_04855"
     CDS             complement(<1..3526)
                     /gene="dnaE"
                     /locus_tag="P5313_04855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRY79725.1"
                     /translation="MSFVHLHNHSDFSLLDGAAGVKSMVAKAKALGMPGLALTDHGNL
                     FGALEFYKECRAQGVNPIVGSEFYVAGASRFEKTGTESGNKYYHLVLLAKDAEGWSNL
                     VKLSSASYVEGFYYKPRIDDEILVRHARGLVCSTACIAGEIPQLILMGRAEAAEAKVL
                     RYRELFGADSFFLELQDHGIPEERTVNAALVALGRKHGIPLLATNDMHYLDRPDASAH
                     DILLCVGTNRKVSDRDRMRFPGTEFYMKTPEEMAALFAEVPEALSNTLRINEMVRLTI
                     PQPGPLLPEFEIPPDFGPSGDARAEEPAARVRDAVARMDDRTICQPHPEYGKRECSDL
                     APEQRRALESRLAQPVTRYFIHLSNEGFRRRYGEGTGEQRDRLDFELAVIILMDFTGY
                     FLIVADFINWAKDHGIPVGPGRGSGAGSIVAYSLRITDIEPLKYNLLFERFLNPERVS
                     MPDFDVDFCFERREEVIDYVTRKYGTDRVGQIITFGTLKAKAVIKDVARALDIPFEDS
                     NNIAKLVPDDPKMTLAKAFEQEPKLRALAEDPRYTELFEAARKLEGRHRHASFHAAGI
                     VIGKTALTDYVPLYRDPKTGALATQFTMDQLEACGLVKMDFLGLKTLTLIQNTLALVA
                     KRGIELTEESIPEDDAATFRLLGEGKSTSIFQFESSGMQGVLKQAKPDRIEDLIALNA
                     LYRPGPMDNIPQFVDCKFGREPIQFPHPVLEPILKETYGVIVYQEQVMQAAQLLAGFS
                     LGGADLLRRAMGKKKAEEMAKQRALFVKGCAEKNSIPEARANEIFDLLDKFAGYGFNK
                     SHAAAYAVVAYRTAYLKANFPAEFMAANLTNEISNPDKMTEYIGEARSMGIEILPPDV
                     NRSETRFTVSEGRIVYGLLGIKGVGEGLVRSILEERERNGPYKDFIEFLERVGMGQMN
                     KKSLEALVLAGCFDSLGHTRQELVLNIDRAVEYVQRKEEARAFGQASLFDGSGEAELP
                     PFAYESSPEYPRKEILQREKDLLGFYFSGHPMDEFRKLWERCADLDLSRPDRAAPKRK
                     YTIVAQLTEWRETLTKSGRRMAFGAVGDFRGSLPIVVFANALEQYRDRFLPDAVLCLR
                     GEVDTSRSEPGFKVDEVLDPSQLREKSYRQVHLRLAGVRAEEDLWDLRDALFESAGPC
                     SVVFHVPGTGGEVTVLPHSGISCAPLPEV"
     gene            complement(3725..5137)
                     /locus_tag="P5313_04860"
     CDS             complement(3725..5137)
                     /locus_tag="P5313_04860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY79726.1"
                     /translation="MNQKPSDPLILRLIQSGKLEYILKGAGIFLRDYSTGQGMCNTLW
                     KECGYGESEMTRERWIELLHPEDRARVVEAVGLLQTGKADFFSEEYRFRDVRDRYRWV
                     RSRAIVLERTAEGVPSLYLGIDNEITDLMARIEAERTEREEMERRYLEAEALRQASTV
                     ITAGVNPGESIGRILAQSAGIVSADAVFIWAVTEDGLDCMGARGADSLPPIPQDRMPR
                     TFAWVLQERRPRRTFGRPISRGDVIYRDSLYMPIVSRGRVLGILEFLGKEPRALGRRA
                     EGPAAVFADSAAVALENALEFRKLDREAGLDWLTNLPTRRRFDFRARAYLEGPAEDTC
                     FCVVMMDLDRFKAVNDTFGHKAGDQGLAAAARVCREALRSTDLVCRYGGEEIALLLPG
                     ADGKAGHAVAERIRTGVEALRFPDYPEMRLTVSLGVRVSCGGTRDLSVLLAESDQALY
                     RAKAEGRNRVILSDPEAPTD"
     gene            5250..6011
                     /locus_tag="P5313_04865"
     CDS             5250..6011
                     /locus_tag="P5313_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRY79727.1"
                     /translation="MELDLFRGLRDADYRSRGLIVAEGRLVVEKALEAGLRIRALVCV
                     PSARGEWESRAEGRFEVRVLPRSEIETLAGFPFHRGVLALAERPEVPALEPGNLPPGN
                     LLWLWNVTDPDNLGTLIRSAAALGAGGVLLGPGCADPLGRKALRTSMGNALVRPLRRA
                     ELDTLPRLRAAGLRLFAAALTPDARPPESLPAGGTSVLVLGNEGWGLPAEVSDACDER
                     VAVPMARDVDSLNVGAAGAILMYVLFGPRGSAAAV"
     gene            complement(6029..6988)
                     /locus_tag="P5313_04870"
     CDS             complement(6029..6988)
                     /locus_tag="P5313_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRY79728.1"
                     /translation="MSAEIDRGTLLRRASLIALFGNAILALVKIGTGIWAGSLAVLGD
                     GIDSSTDVVIAGLSLFVAGVVARPADSDHPWGHGRAETVATTALAFILFFAGGQLVLS
                     GLGNLLRPEPLRIPDPAALVVTAISMAGKGFLAWTQFRYGRRADSAMLRANGKNMAGD
                     ILISAAVLAGLFLAVAAGIPAADPVAAVLVGLWVVRNAFGIFREANLELMDGNADTSL
                     YTLLFDAVRAVPGAGKPHRARMRRIAGAWDIDLDIEVDGAMTVRDSHELAGAVEREIR
                     RRVENVLDIVVHVEPAGASGSESHEGGEGYGLDEGSLDWDGEL"
     gene            7133..8233
                     /locus_tag="P5313_04875"
     CDS             7133..8233
                     /locus_tag="P5313_04875"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRY79729.1"
                     /translation="MEARSILPPVDVMDRAFRERDAEFDGVFYAAVTTTRIFCRPSCP
                     ARKPGEGRVEFYASPREALFAGFRPCLRCRPLEAGGRAPDWVRGLLDAVEEDPERRFR
                     DQDLRELGVDPAGARRWFKRAYGMTFQAYNRARRLGRAFDSLKRGGSVDDAALGYGWE
                     SFSGFREAFGKTFGAPPGTVTAEADGAARAVTGSRDFVRLGWIETPLGPMVAGAVDEG
                     LCLLEFSDRRMLETQLDTLRSRFRLPLAPSESPHFELLRRELSAYFAGSLREFTVPLV
                     LRGTPFQERVWRGLLEVPHGSTVSYAALAERIGVPGASRAVGHANGLNRIALIVPCHR
                     VVNADGRVGGYGGGLWRKQALLDLERGARTFG"
     gene            complement(8277..9194)
                     /locus_tag="P5313_04880"
     CDS             complement(8277..9194)
                     /locus_tag="P5313_04880"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HRY79730.1"
                     /translation="MSSFLIALLPVLGWGTWLAVSQDVRSPNPRAKTLYAVLANLAVA
                     ALVFLFTGSAWDRPSFWPVFLGGLLWSVGGVAAFAAAELIGLARGAGIWVPVNLAVGL
                     GWGVLLFRELEGASAGLKVLTALAAAILTGGVLLILSARKGETPATGNGAGGDAGGTG
                     IGRTVKGMLLAALAGLFFGTYFIPIRASGVSAWSAALPMSLGMAAGTLALCLAYPAAP
                     IMARPGHYLRSGLSGALWAAGNYGMLLLTERIGTGRGFSVAQLNIIVSAVAGIYVFRD
                     PPPRTRAARTALAGCLVAAAGGVLLGLVK"
     gene            9554..10015
                     /locus_tag="P5313_04885"
     CDS             9554..10015
                     /locus_tag="P5313_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY79731.1"
                     /translation="MYTNRWEPSTALDFFDSLDVFRDEMERWVEGVRSPEVRGIFDRT
                     LTPAVDVIETRDAFVVNVDVPGVSKKDLNVTLTGTVLSIQGEKKEEEKDDKGRKFFRK
                     ETWAGGFRRTLDLPETADPEKVSADLKDGVLTVTVQKREEVKPRLIAVNVN"
     gene            10035..10391
                     /locus_tag="P5313_04890"
     CDS             10035..10391
                     /locus_tag="P5313_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY79732.1"
                     /translation="MEATKSLTAPKRRFVNPACSICEDEGTVMVTVEMPGVRKEDLEI
                     KIEGNELVILGKRPESAPEGTYLLRERRIGDFRKTFTLDESIDHEGVDGKLADGVLAL
                     TLKVKAAALPRKIAIR"
     gene            10496..10786
                     /locus_tag="P5313_04895"
     CDS             10496..10786
                     /locus_tag="P5313_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79733.1"
                     /translation="MGVPVTQKSHPLSLERRQYLCERYGLSPSDLALLLEDLWAFTEE
                     TPERYVLRRHSELQKRGWTNDGIFKRIAGELAAGRFSAEPLSLRQIRRIIYG"
     gene            10815..12647
                     /gene="glmS"
                     /locus_tag="P5313_04900"
     CDS             10815..12647
                     /gene="glmS"
                     /locus_tag="P5313_04900"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRY79734.1"
                     /translation="MCGIIGYTGPRKALPLLLQGLHLLEYRGYDSAGIALATEGSLRV
                     YKKEGKLENLEAVLPSSSAAKSGIGHTRWATHGIVSDKNAHPHLGSKGRVAVAHNGIL
                     DNWAEIRRELESEGVVFTSDTDSEVIAQLVERELETRKPTDAVIAALKRISGTYGIAV
                     TFADKPALVIGARNGSPLAIGLGDGEAFLASDPSAFASHTRKAVFLQDGDIAVLQPKS
                     WEIRNLRGELSDREAETLPARTAEGDKGVHPDFFLKETFEQPQAVARALGNGGRLVKD
                     LGTAKLGGLDMAPREIQGIREIVFVAMGTALHAAQIGARIVETWARIPSRAEDASELR
                     SANPIVHRDALYVAVSQSGETADTLAAVREIQMKGGRTIGIVNGVGSTLARLCGSGIY
                     VHAGPEHSVAATKSWSAQIVCCALLALTVGRSRYLSLGEGREAVEELEALPGKIEEAL
                     ALNARVDELAKRWSGARSTLFLGRGVLYPAALEGALKLKEISYVHAEGFAGGALKHGP
                     LALVSPEVPTFALLADGETFDRTLSNMSEVRARRGPVIAVTNREDPRLADAADEILVV
                     PRSLEVLAPVLETIPLQLYARAVASELGRDVDRPRNLAKSVTTE"
     gene            12740..13240
                     /locus_tag="P5313_04905"
     CDS             12740..13240
                     /locus_tag="P5313_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRY79735.1"
                     /translation="MSNSPATEFKVDQRVVYPSQGVGRITSITEKKFKDVSVLYYIIY
                     LEFSDMTVMVPVDKARDLGIRPIVPKDEALKALNFIAEEYAPIPSDWKLRYQMNLDLL
                     KKGSVMDIASVVRSLYHRSKIKELPILERKLYDSALNLLQDEVSFSLAKPKDDIAEII
                     RQKLEG"
     gene            complement(13245..15245)
                     /locus_tag="P5313_04910"
     CDS             complement(13245..15245)
                     /locus_tag="P5313_04910"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRY79736.1"
                     /translation="MIALDAEPGGFTLSVDGHRVFSHSERRPCAELGVSRTAEGGKRG
                     AAPWEGGIKWTPLRRFRAEEAAPGSAVIDFEGRLRLSARHADGILRLTLERPRDRYDR
                     FRFRLAAVPGEAILGCGERFGPLDLKGRSVPLWVEDRGGARELCPLPIPANLKRNPRR
                     IRSTYLPLPCFVSTDRYWVFADTDSFARFDFRSSRQTVLEFRKTPREISLGRGGSAAE
                     TVSSLACRVGRPPPIPPWTRKGAILGVGGGAGEVLRKLDAARTAGAPVAAVWVRDWCG
                     ARDTRQGLQPRWNWEADPDLYPDLPGFIASLRSEGVRFLGYVNPFLASEGNLCAEARE
                     RGLLVRSPGGGEYRFGAGSQTAVMPDLTNPRAFRWVKDVLRSRMIDPGMSGWLADLGE
                     CLPDDAVLHSGEKAQDLHNLWPVLWARAGREAIEEAGASGEIAFFQRSGWAGSSTLAS
                     GFWAGVQLSDFSDAAGLSSVVPAGLSAGLSGAGFWHFDAGGCVSGAFGARKPECLERW
                     FEMAAFSPVFRTQEGNKPLSDCQYWTDPACLRHFVRMAGIFAALEPYHAAAARECSEA
                     GLPPIRHLWTHYEEDPEVRFLDHQYLYGRDLLVCPVFRKGQELKDSWLPPDRWVHFWT
                     SRTFRGGPVSLEAPLGYPLVFYREESRFAPLFDSIRRTARRP"
     gene            complement(15269..16162)
                     /locus_tag="P5313_04915"
     CDS             complement(15269..16162)
                     /locus_tag="P5313_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY79737.1"
                     /translation="MEVAVLLGSKDSQTALALLKNLGKAGISAYALKLGPSWEDESRK
                     PLEFRIRKASHILLAVSGESVHSTWFAFTGGWCLGNDKRLAVYREDASLRIPGYLTGV
                     TVLDSLDRAAAYYETERAEWLVREGRSRARASLLELGIPYRSESLAECCRQGDVKAVE
                     LFLRAGMIPDMRDRAGVTLLGLALRSKHQAVAELLVEHGAALDLQSEDRGYSALMDAA
                     AAGSADAVDYLLKRGANPNLSSKDGQTALVIAVGRNDVPLCRKLLSYGADPDLKDKLG
                     FSARKYAELFHNPEMVRLFAQ"
     gene            complement(16293..17687)
                     /locus_tag="P5313_04920"
     CDS             complement(16293..17687)
                     /locus_tag="P5313_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79738.1"
                     /translation="MSDKTEAMGAQEAGFPTMSSYGMGKFLAEFLTGGFAALVFKYYE
                     TELGLSAGLVAAGVIVYSLWNAVNDPLIGYLTARPTPWAARLGRRFPWIAAGTVLCAL
                     SFVLIFAPPGSPAGGTHPGLLFGWLVFSVCLYDGLYSVWELNYQSVFPDRFRGTAERA
                     KAAGVATVVGVLGIAGGAILPTLVIRYGDPASYTANGWIFAAVALVSALLLLPGVRET
                     PSMIRRYLEQTARTSEEPGFFARLREALGDRDFLAFILLFFFYQSATISMTASIHYVG
                     DYVLGGRSTTFIFAGMLLGALAAVPIWTVLLRRRGDVQGLLQAAALVLAVFCLPLLAA
                     RTYGAFVAAMFFWGTAFGGFWLLIGPALADVVDGIVVRTGKREDGVYLGFRAFAGRLS
                     YAVQALSFWAVHAATGFARDPRSPAAVAGIRVHTALLPALFLVAGVLVFRSLNTLSKA
                     KAESNRAALRERGL"
     gene            17970..18488
                     /locus_tag="P5313_04925"
     CDS             17970..18488
                     /locus_tag="P5313_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79739.1"
                     /translation="MNRKRITALLALATLTAAVFAASAAWGSAGAAAVPAGELTLESM
                     LAYAIQDEYLARAEYIAIQQRYGIQRPFSNIQRSEENHIAWLVDAYNTHGLPVPKDEA
                     GRYVRIPASLKDAFQTGVDAEVANIAMYDRFLATPLLARPENAALRDLFTRLRDASQN
                     HLQAFRTGLSRY"
     gene            complement(18561..19367)
                     /locus_tag="P5313_04930"
     CDS             complement(18561..19367)
                     /locus_tag="P5313_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79740.1"
                     /translation="MTRPGLPGPGPSGRSDPARRGFPASVLPGALLVLAAWQVLHLGL
                     GTSAVPSPGETFATLADLARRDLAVHLAASLARVLAATALQILLGSALGIALGASRRA
                     EFLLGSAVRILHPVPKVAFLPVFMILFGIGDLSKILFVAAVAVFQTLVSVRDGIAEIP
                     GRLFLAARSLGMGRSELLRHLYIPAVLPRIFTSLRLGLGMGVAALFFAENYATRYGAG
                     YLIMNAYAMADYRTTFAGIAALSLLALGLFGLADLAERRLCPWLRAGKGE"
     gene            complement(19364..20164)
                     /locus_tag="P5313_04935"
     CDS             complement(19364..20164)
                     /locus_tag="P5313_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY79741.1"
                     /translation="MIEARGLTVDYPRGRRAGRSAGPAFDRALDGLDLSLPRGSSLAV
                     VGPSGCGKSTLLYAIAGLVPAAGGSLHVDDRPPAERSGTALVFQDYGLFPWKTALQNV
                     ELGCRGGSRAGKEAQDRRSRGADRDRALSLLEHFGVGHGADKYPAELSGGERQRTAIA
                     RALAAEPDLLLLDEPSSSLDALSKESFQRAVLGLCSHSDSPKNLTVVLVTHDIEEAVF
                     LGSSVAVMERGRIRAVLDNPLFGDPSLRERLEYYEFCLGVRRLLEGEP"
     gene            complement(20184..21131)
                     /locus_tag="P5313_04940"
     CDS             complement(20184..21131)
                     /locus_tag="P5313_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79742.1"
                     /translation="MPAALPAPRGPRSIRSKPRSGRSFRLSAALFLLAPAALPLSAAG
                     PELRVGLMPAVDSAPALLAEKRGYFAREGVSVELVVFPNATERQVALQSGAVDGSMSD
                     LVAFVYNVQGGFDLRITSSTDGSFPFLVRPGFPEGRKASVAMMEVSVSNYLADAWLAG
                     RYEIEKVFITDIPARLEMIKRGKVDMACLPEPIASMGQLAGLEKRVFENKDDFYPDVM
                     VFTARAAGAKREAIAAFNRAVDRAVADIRADPALARDILVERLRLDPRVRDLMALPEY
                     RPTRLPSRGYLDKVSAWIEGLQGKPVTLDWGRYLLDASR"
     gene            21259..22167
                     /locus_tag="P5313_04945"
     CDS             21259..22167
                     /locus_tag="P5313_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRY79743.1"
                     /translation="MELLECGLPALIRDLWIAARPFSLSLALYATSVPLALAFRVGIL
                     PGDRPWVGAFRALLVTLSGLAVQAATNLINDYFESGIKGAPEAERKYRFLGRDRGAFE
                     ILVFLLALACFGFTALAGIYLVLTVTPVLLWIGALGLAGGYCYTGEPVVYKRFGLGAV
                     LSFALLGPLMNYGAWLGVGGTPSWLPVLVALPTSLLIPAMMLSNEIRDVERDAELGIR
                     TLTVRIGRKAGQAVYDGLLLATFGWAAALVLLDLAPAATLASWAALPLAVRARVRVAE
                     RRRDAIPATNLLHLVFGALYIGGLLW"
     gene            complement(22279..22908)
                     /locus_tag="P5313_04950"
     CDS             complement(22279..22908)
                     /locus_tag="P5313_04950"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY79744.1"
                     /translation="MPVRRRIGSTASEERLERLIEERLKPGSDRERIDGRIWDLFGED
                     WAVMFTDLSGFSRNVAKFGIIHFLQTIYESERILVPVLEDNDGILLKTEGDSMLVIFR
                     NPAKALHAALEMQAACDAYNRTRSDEEKVLLCVGLGYGKMLRIGDADVFGREVNASSK
                     LGEDVAKAGEILATDAVRQAVAGQPYEFRELEETPTGVGKAWKLVYGGQ"
     gene            complement(23052..23261)
                     /locus_tag="P5313_04955"
     CDS             complement(23052..23261)
                     /locus_tag="P5313_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRY79745.1"
                     /translation="MKKIVTIEGMSCGHCVMRVKKALEGVPGVTKADVDLLKGEATVE
                     GEGFGGPQLEGAVNHAGYRAVKVVP"
     gene            complement(23476..24909)
                     /gene="gcvPB"
                     /locus_tag="P5313_04960"
     CDS             complement(23476..24909)
                     /gene="gcvPB"
                     /locus_tag="P5313_04960"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRY79746.1"
                     /translation="MNLRPEPLAYELSAPGRCGAALPEPDVPETALPEGLLRDDLPLP
                     EIDELTAVRHFTRLSQKNYSIDTEFYPLGSCTMKYNPKANEAAASLPGWRTTHPLAGA
                     EFSQGALELMYRLQAMLVEIGGFKAASLQPAAGAHGELAGVLMIRKWHRDRGDLKRVK
                     MLIPDSAHGTNPATCSMVGFETVTIPSDADGGVDLAALAAALDDTVAGIMITNPSTLG
                     LFERNIEKIAKLVHDAGGLVYGDGANLNAITGIFRPGDAGIDVMHFNLHKTFSTPHGG
                     GGPGSGMVAAGERLADYLPGPIAVKKDGRFDMAMPKAGIGRLKAFHGNFGVLVRAYTY
                     IRMLGAPGLRRLAETAVLNANYLKAKVEKVYPLKYGRGCMHEFVAMGNFVEGVHTLDI
                     AKRLIDYGFHPPTIYFPLIVPEALMIEPTETETRETLDAFADALLKIAEEARTEPELL
                     HSAPHVTPVGRLDEVAAARCPILCYRG"
     gene            complement(24906..>25130)
                     /locus_tag="P5313_04965"
     CDS             complement(24906..>25130)
                     /locus_tag="P5313_04965"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRY79747.1"
                     /translation="VLRFAIAFMILFLVKTPVPAQSIYDKLSRRCVVPGLPLSRYWPD
                     RTHELLVCVTEMNTKEQIDLLAASLKEASK"
BASE COUNT         4112 a         8535 c         8614 g         3869 t
ORIGIN      
        1 tgacctccgg caggggcgcg cagctgatcc ccgagtgggg caggacggtc acctcccccc
       61 ccgtgccggg gacgtggaag accacggagc agggcccggc ggactcgaag agggcgtccc
      121 ggaggtccca gaggtcctcc tcggctcgga ccccggccag ccgcaggtgg acctgccggt
      181 aggatttctc ccggagctgg gaggggtcca ggacctcgtc caccttgaag cccggctcgg
      241 accggctggt gtccacctcc ccccgaaggc agagcacggc gtccggcagg aagcggtcgc
      301 ggtactgctc cagggcgttg gcgaagacga cgatgggcag ggagccccgg aagtccccca
      361 cggccccgaa ggccatcctg cggcccgact tcgtgagggt ctcccgccac tccgtcaact
      421 gggccactat cgtgtacttc cgcttgggag ccgcccggtc cggccgggac aggtccaggt
      481 ccgcgcagcg ttcccagagc ttccggaact cgtccatggg gtggccggag aaatagaaac
      541 ccaggaggtc cttttcccgc tggaggatct ccttccgggg atactccggg gaggactcgt
      601 aggcgaaggg cgggagctcc gcctccccgg atccgtcgaa gaggctggcc tgtccgaagg
      661 cccgggcctc ctccttgcgc tggacgtact ccacggcccg gtcgatgttg aggaccagct
      721 cctgccgggt gtgccccagg gagtcgaagc acccggccag gaccagggcc tccagggact
      781 tcttgttcat ctgccccatg cccacccgct ccaggaactc gatgaagtcc ttgtagggtc
      841 cgttccgttc ccgctcctcg aggatggagc ggaccagacc ctcccctacc cccttgatgc
      901 ccagaagccc gtagacgatc cgaccctcgg atacggtgaa cctcgtctcc gaacgattga
      961 cgtcgggggg caggatctcg atccccatgg accgggcctc cccgatgtac tcggtcatct
     1021 tgtcggggtt cgagatttcg ttggtcaggt tggcggccat gaactccgcg gggaagttgg
     1081 ccttgaggta ggccgtgcgg taggccacca cggcgtaggc cgcggcgtgg ctcttgttga
     1141 agccgtagcc cgcgaacttg tccaggaggt cgaagatctc gttcgcccgg gcttcgggga
     1201 ttgagttctt ctcggcgcaa cccttcacga agagggcccg ctgcttggcc atctcttcgg
     1261 ccttcttctt gcccatggcg cgccggagga gatccgcgcc tcccagggag aagcccgcca
     1321 ggagctgggc cgcctgcatg acctgctcct ggtagacgat gaccccgtag gtctccttga
     1381 ggatgggctc cagcacgggg tgggggaact ggatgggctc tcggccgaac ttgcagtcca
     1441 cgaactgggg gatgttgtcc atggggcccg gccggtacag tgcgttcagg gcgatcaggt
     1501 cctcgatgcg gtcgggcttg gcctgcttga ggaccccctg catgcccgag ctctcgaact
     1561 ggaagatcga ggtgctcttc ccctccccca ggaggcggaa ggtggccgca tcgtcctcgg
     1621 ggatcgactc ctcggtcagc tcgatccccc gcttggccac cagggccagg gtgttctgga
     1681 tcagggtcag ggtcttgagg cccaggaagt ccatcttcac gaggccgcag gcctccagct
     1741 ggtccatggt gaactgggtg gccagggcgc cggtcttggg gtcccggtag aggggcacgt
     1801 agtccgtcag ggcggtcttg ccgatgacga tccccgcggc gtggaaactg gcgtggcggt
     1861 gacggccctc cagcttccgg gcggcctcga agagctccgt gtaccgggga tcctcggcca
     1921 gggcccggag cttgggttcc tgctcgaagg ccttggccag ggtcatcttc gggtcatcgg
     1981 gcacgagctt ggcgatgttg ttggagtcct cgaaggggat gtccagggca cgggccacgt
     2041 ccttgatgac cgccttggcc ttgagggtcc cgaaggtgat gatctggcca acccggtccg
     2101 tgccgtactt ccgggtgacg tagtcgatca cctcctcccg acgttcgaag cagaagtcca
     2161 cgtcgaagtc cggcatggac acccgctcgg ggttcaggaa gcgctcgaac aagaggttgt
     2221 acttgagggg ctcgatgtcc gtgatccgca gggagtaggc cacgatggac cccgccccgg
     2281 aaccgcgccc gggtcccacg ggtatgccgt ggtccttggc ccagttgatg aagtccgcga
     2341 cgatcaggaa gtagcccgtg aagtccatga ggatgatgac cgccagctca aagtccaggc
     2401 ggtccctttg ctcgcccgtg ccctcgccgt agcgccggcg gaagccctcg ttggacaggt
     2461 ggatgaagta gcgggtgacc ggctgggcca ggcgggactc cagggccctc ctctgctcgg
     2521 gcgccaggtc cgagcactcc cgcttgccgt actcggggtg gggctggcag atggtccggt
     2581 cgtccatccg ggccaccgcg tcccggaccc gggcggcggg ctcctcggcc cgggcgtccc
     2641 cggagggtcc gaagtccggg gggatctcga actccggcag gagggggccc ggctggggga
     2701 tggtcagccg gaccatctcg ttgatccgga gggtgttgga cagggcttcg ggaacctccg
     2761 cgaagagggc ggccatctcc tcgggagtct tcatgtagaa ctcggtcccc gggaagcgca
     2821 tccggtccct gtccgagacc ttgcggttcg tacccacgca cagcaggatg tcgtgggccg
     2881 aggcgtccgg gcggtccagg tagtgcatgt cgttggtggc cagaaggggg atgccgtgct
     2941 tccggcccag ggctaccagg gccgcgttga cggtccgctc ctccgggatg ccgtggtcct
     3001 ggagctccag gaagaaggaa tccgccccga acagctcccg gtaccggaga accttggcct
     3061 ccgccgcctc ggcccgcccc atcaggatca gctgggggat ctccccggcg atgcaggcgg
     3121 tggagcagac gaggccccgg gcgtgccgga ccaggatctc gtcgtcgatc cggggcttgt
     3181 agtagaagcc ctccacgtag gaggcgctgg acagcttgac aaggttgctc cagccctcgg
     3241 cgtccttggc caagaggacc aggtggtagt acttgttccc gctctcggtg cccgtcttct
     3301 cgaagcgcga cgcgccggcc acgtagaact cggagccgac gatggggttc accccctggg
     3361 ccctgcattc cttgtagaac tccagggccc cgaagaggtt cccgtggtcc gtcagggcca
     3421 ggcccggcat gcccagggcc ttggccttgg ccaccatgga cttgaccccg gcggcgccgt
     3481 ccaggaggga gaagtcggaa tggttgtgga ggtggacgaa ggacatcggg tggagtatag
     3541 cgcggagccc tgcagctggg aaggctgcgg aagaacgttt cccggaaaaa ttcgacagga
     3601 tggtcaggat tagaaaagga tatacaggat aatgaattaa ttaaagcaag aagctcaagg
     3661 acgaatcctg ttcatcctta ttcaatcttg tccatcctgt ctatcccatg gttccaaggc
     3721 gccctcagtc ggtcggagcc tccggatccg acaggatcac ccggttgcga ccctcggcct
     3781 tggcccggta cagggcctgg tccgactccg cgaggaggac cgacaggtcc cgggtccctc
     3841 cgcaggacac ccggactccc aggctcacgg tcagccgcat ttcggggtag tccgggaacc
     3901 ggagggcctc cacccccgtc cggatgcgct ccgccacggc gtgaccggcc ttcccgtccg
     3961 caccgggcag gagcagggcg atctcctccc ctccgtagcg gcacacgagg tccgtggagc
     4021 gcagggcctc ccgacagacc cgggcggcgg cggccaagcc ctggtccccg gccttgtgcc
     4081 cgaaggtgtc gttcacggcc ttgaaccggt ccaggtccat catcacgacg cagaagcagg
     4141 tatcctccgc cggaccctcc agataagccc gggcgcggaa atcgaagcgg cggcgggtcg
     4201 gcaggttggt cagccagtcc aggcccgcct cccggtccag cttccggaac tccagggcgt
     4261 tctccagggc caccgccgcg gaatccgcga acaccgcagc cgggccttcc gccctgcgtc
     4321 ccagggctcg gggctccttg cccaggaact ccaggatgcc caggacccgg ccccgggaca
     4381 cgatgggcat gtacagggaa tcccggtaga tcacgtcccc ccgggagatg ggccggccga
     4441 aggtcctgcg gggacgcctc tcctggagca cccaggcgaa ggtacggggc atccggtcct
     4501 gggggatcgg gggcaggctg tccgcccccc gggctcccat gcaatccagc ccgtcctccg
     4561 tgaccgccca gatgaacacg gcgtccgcgg acacgatccc cgcgctctgg gccaggatgc
     4621 ggccgatgga ctccccggga ttgacccccg cggtgatcac cgtgctggcc tgccggaggg
     4681 cttccgcctc caggtagcgc cgctccatct cctcccgctc ggtccgttcc gcctcgatcc
     4741 gtgccatcag gtccgtgatc tcgttgtcga ttcccaggta gagggagggc accccctccg
     4801 cggtcctctc caggacgatg gcccgggagc gtacccagcg gtaccggtcc cggacgtccc
     4861 ggaagcggta ctcctcggag aagaagtcgg ccttgcccgt ctgcaggagt cccaccgcct
     4921 ccacgacccg ggcccgatcc tcgggatgca ggagttcgat ccagcgctcc cgggtcatct
     4981 cggactcgcc gtagccgcat tccttccaga gggtgttgca catgccctgt ccggtggagt
     5041 aatcccgaag gaagattccc gcgcccttga gtatatattc cagttttccg gattgtatca
     5101 gacggagtat caaggggtcg gaaggcttct ggttcatctg acttctttct atagcacgct
     5161 tttcaatggt agtcgatact atatactcgc ccggaggaat tgcaaatatt tcttcaagga
     5221 attccgtttg tcacgatgcg gagataacca tggagctgga ccttttccgc ggcctacggg
     5281 acgcggatta tcgcagccgc ggcctgatcg tggccgaagg ccggctggtt gtcgaaaagg
     5341 ccctggaggc gggtctgcgg atccgggccc tggtctgcgt gccctcggcc cggggcgagt
     5401 gggaaagccg cgcggaaggc cgcttcgagg tccgggtcct tccccggtcc gagatcgaga
     5461 ccctggccgg attccccttc cacaggggtg tccttgccct ggcggagcgc ccggaggttc
     5521 ccgccctgga acccggaaac ctacccccgg ggaaccttct atggctctgg aacgtcacgg
     5581 acccggacaa cctgggaacc ttgatacgga gcgccgcggc cctgggggcc ggcggggtgc
     5641 tcctggggcc cgggtgcgcg gatcccctgg gcaggaaagc cctccggacc tccatgggca
     5701 atgccctcgt ccggcccctc cgccgggcgg aactggatac ccttccccgg ctccgggccg
     5761 cggggctccg gctgttcgcg gccgccctga cccccgatgc ccgcccgccg gaatccctgc
     5821 ccgccggagg cacctcggtc ctggtcctgg gcaacgaggg ctggggcctg cccgcggagg
     5881 tctccgacgc ctgcgacgag cgcgtggccg ttcccatggc ccgggacgtg gattccctga
     5941 acgtgggagc cgccggagcc atcctcatgt acgtcctgtt cggaccccga gggtcggcgg
     6001 ccgccgtctg acctcccctg tccaccgtct acagttcacc gtcccagtcc agactcccct
     6061 cgtccaatcc gtagccctcc ccgccctcgt gggattccga ccccgaggcc cccgcgggct
     6121 ccacgtggac gacgatgtcc aggacgttct ccacccggcg tcggatctcc cgctcgaccg
     6181 ccccggccag ctcgtgggaa tcccggacgg tcatggcccc gtccacctcg atgtccaggt
     6241 cgatgtccca ggctcccgcg atgcgccgca tccgggccct gtggggcttt ccggcccccg
     6301 ggaccgcccg gaccgcgtcg aacaggaggg tatacagcga ggtgtccgcg ttcccgtcca
     6361 tcagctccag gttggcctcc cggaatatcc cgaaggcgtt ccggaccacc cacaggccga
     6421 ccaggaccgc ggcgaccggg tccgcggcgg gaatcccggc ggccacggcc aggaaaagcc
     6481 cggccaggac cgcggcggag atcaggatgt ccccggccat gttcttgccg ttcgccctca
     6541 gcatcgcgct gtccgccctg cgcccgtagc ggaactgggt ccaggccagg aatcccttcc
     6601 cggccatgga tatcgccgtg actaccaggg ccgcggggtc cgggatccgg aggggctcgg
     6661 gccggagcag gttccccagg cccgagagga cgagctggcc ccccgcgaag aacaggatga
     6721 aggccagggc cgtggtggcc acggtctccg cccgtccgtg tccccagggg tggtcggagt
     6781 ccgcgggccg ggcgacgacg cccgccacga agagggacag ccccgcgatc acgacgtccg
     6841 tggaggaatc gatcccgtcc cccagcacgg ccaggcttcc ggcccagatc ccggtgccga
     6901 tcttgaccag ggccaggatc gcgttcccga acagggcgat gagggaggcc ctccgtagga
     6961 gggtccccct gtcgatttct gcactcaaga tccgtccccc ttcacaagct ccggaacact
     7021 accgacttct taaatcggct ccactataga tggttttcgg gttccctgtc cattttccgg
     7081 tggatgaccg ggcggttccg gttgtatcgt tgggtgcagg caaggaggag ctatggaagc
     7141 gcgatcgatc cttccccccg tcgacgtgat ggaccgggcg ttccgggagc gggacgcgga
     7201 gttcgacggg gtgttctacg ccgcggtcac gacaacccgg atcttctgcc ggccctcctg
     7261 tccggcccgg aagcccgggg aggggcgggt ggagttctac gccagccccc gggaggccct
     7321 gttcgcgggg ttccgtccct gcctgcgctg ccgtcccctg gaggccgggg gccgggcgcc
     7381 ggactgggtc cggggtctcc tggacgcggt ggaggaggat ccggaacgcc ggttccggga
     7441 ccaggacctg cgggaactcg gggtggatcc cgcgggagcc cggcgctggt tcaagcgggc
     7501 ctacggcatg accttccagg cctacaaccg ggctcggagg ctcggccgcg ccttcgactc
     7561 tctcaaacgg ggggggtccg tggacgacgc ggccctggga tacggctggg agtccttttc
     7621 cggcttccgg gaagccttcg gcaagacctt cggggctcca ccgggaaccg tgaccgccga
     7681 ggccgacgga gccgcccggg cggtgacggg ttcccgggac ttcgtgcgcc tgggctggat
     7741 cgagactccc ctgggcccca tggtcgcggg ggccgtggac gagggcctgt gcctcctgga
     7801 gttctcggac cgtaggatgc tggaaaccca gctggacacc ctgcgctcgc ggttccgcct
     7861 gcccctggcc ccctcggaga gcccccattt cgaactactg cgccgggagc tgtccgccta
     7921 cttcgcgggt tccctccggg agtttacggt acccctggtc ctgcggggaa cgccgttcca
     7981 ggagcgcgtc tggcgcggcc tgctcgaggt tccccacgga agcaccgtaa gctacgcggc
     8041 cctggcggag cggatcgggg ttcccggggc gtcccgcgcg gtgggacacg ccaacgggct
     8101 caaccgcatc gccctgatcg tgccctgcca ccgggtggtg aacgccgacg gccgggtagg
     8161 aggctacggc ggggggctct ggcggaagca ggccctcctg gacctggagc ggggggcgcg
     8221 caccttcggg tgagtcattt gagccgattt taaaagtgcc cgactttaaa atcggctcat
     8281 ttgaccaggc cgagcaggac gccccccgcc gcggcgacca ggcatcccgc cagggcggtc
     8341 cgggcggccc gggtccgcgg aggcgggtcc cggaagacgt agatcccggc cacggcgctc
     8401 acgatgatgt tcagctgggc gacggaaaag ccccggcccg tgccgatccg ctccgtgagc
     8461 aggagcatgc cgtagttgcc cgcggcccat agagccccgg acagcccgga gcggaggtag
     8521 tggccggggc gcgccatgat cggtgccgcg ggataggcca ggcagagcgc caaggtacct
     8581 gcggccatcc ccaggctcat gggaagggcg gcgctccagg cggaaacccc gctggcccgg
     8641 atcgggatga agtaagtgcc gaagaagagg cccgccaggg ccgccaagag catgcccttc
     8701 accgtccgcc cgatgcccgt gccgccggcg tctccgcccg cgccgttccc ggttgccggt
     8761 gtctccccct tgcgggcgga gaggatcagc aggacacccc ccgtcaggat cgccgcggcc
     8821 agtgccgtca ggaccttgag ccccgcggag gcgccctcga gctcccggaa gaggagaacc
     8881 ccccagccca accccacggc caggttcacg ggcacccaga tgcccgctcc ccgggccagg
     8941 ccgatcaact ccgcggccgc gaaggcggcc acgcctccca cggaccagag caggccgccc
     9001 aggaagacgg gccagaagga gggacggtcc caggcggaac ccgtaaagag gaagaccagg
     9061 gccgcgacag ccaggttggc caggacggcg tagagggttt tggcccgggg attcggggac
     9121 cggacgtcct gggataccgc cagccaggtg ccccacccga ggacgggcag gagggcgatc
     9181 aggaagctgc tcatggccgg agtatatcga agggtaccgt cgggggaata gcctgtccgg
     9241 aaacagaacc ccggcccggc gaagtcggag tgtttctttt ttactcactt cgtagggcaa
     9301 tatttacaca ctatttccaa tcgaaacaat tttattggga acctcccgct cctcgtccgt
     9361 cccggaacgg gaacaagtcg aaggtgtgtt gtagttattt attttttatt cctgcaatta
     9421 cgtgcatcgg cattcgcggc gaccggaccg agcaaagttg gcacgaatcc tgctttacag
     9481 taagcgaaag aaaagacagc ggcgccgagg cgccgacacc ggggcagcgc cccgaaatac
     9541 cgaggaggtg gatatgtata ccaaccgatg ggagccgtcg acggctctgg acttcttcga
     9601 ttccctggac gtcttccggg acgagatgga acgctgggtg gaaggggtac ggagccccga
     9661 ggtccggggg atcttcgacc gcaccctgac ccccgccgtg gacgtgatcg agacccggga
     9721 cgccttcgtg gtgaacgtag acgtgcccgg cgtttccaag aaggacctta acgtcaccct
     9781 gaccggcacg gtcctgagca tccaggggga gaagaaggaa gaggaaaagg acgacaaggg
     9841 ccgaaagttc ttccgcaagg agacctgggc gggcggtttc cgccggaccc tggatcttcc
     9901 ggaaaccgcg gatcccgaga aggtgagcgc ggacctgaag gacggggtgc tcaccgtcac
     9961 ggtccagaag cgggaagagg tgaagccccg cctcatcgcc gtcaacgtca actgaaatcg
    10021 gaaaggagga agccatggaa gcgacgaaga gcctgaccgc cccgaagcgg cggttcgtca
    10081 atccggcctg ctccatctgc gaggatgagg gcacggtcat ggtaacggtg gagatgcccg
    10141 gggtccgcaa ggaagacctg gagatcaaga tcgaggggaa cgagctggtg atcctgggga
    10201 agcgccccga gtccgcgccg gagggcacct atctcctgcg ggaacggagg atcggggatt
    10261 tccggaagac cttcaccctg gacgaatcca tcgaccacga gggtgtggac ggcaagctcg
    10321 cggacggggt cctcgccctg accctcaagg tcaaggccgc cgccctgccc cgcaagatcg
    10381 ctatccggta gacagccgtc ctgcaggcaa gacccgagcc cgagctcggg tcttttttta
    10441 cccgttttcg ggcacgttcc ttgacatgcc gggctccgat caccatagtc ccgtcatggg
    10501 cgtaccggtg acacagaaaa gccatcctct gtccctggag cgaaggcagt acctctgcga
    10561 acgctacggc ctttcaccct cggacctggc cctcctcctg gaggacctct gggccttcac
    10621 ggaggagacc ccggagcggt acgtcctccg ccggcactcg gagctccaga agaggggctg
    10681 gacaaacgac gggatcttca agcgcatcgc cggggagctg gccgccggga ggttttcggc
    10741 ggagcccctc tccctccggc agatccggcg catcatatac ggatgaccgg cggcggccgg
    10801 caaaggagcg gaccatgtgc ggaatcatcg gatatacggg cccccgcaag gcgcttcccc
    10861 tgctcctgca ggggctccac ctcctggagt accggggcta cgattccgcg gggatcgccc
    10921 tggccacgga aggctccctc cgggtctaca agaaggaggg caagctggaa aacctggagg
    10981 cggtcctccc ctcctcctcg gcggccaagt ccgggatcgg ccacacccgc tgggcgaccc
    11041 acgggatcgt gtcggacaag aacgcccacc cccacctggg ctccaagggc cgggtggcgg
    11101 tggcccacaa cggcatcctg gacaactggg cggagatccg ccgggagctg gagtccgagg
    11161 gggtggtctt caccagcgac acggacagcg aggtgatcgc ccagctggtg gagcgggagc
    11221 tggagacccg gaagcccacg gacgccgtga tcgcggccct caaacggatt tcgggcacct
    11281 acgggatcgc ggtgaccttc gcggacaagc cggctctggt catcggtgcc cggaacggca
    11341 gccccctggc catcggcctc ggcgacggtg aggccttcct ggccagcgat ccctcggcct
    11401 tcgcctccca cacccgcaag gcggtgttcc tgcaggacgg ggacatcgcg gtgctccagc
    11461 ccaagtcctg ggagatccgc aatctccggg gggaactctc ggaccgggag gccgagaccc
    11521 tgcccgcccg caccgcggag ggcgacaagg gggtccatcc ggacttcttc ctgaaggaaa
    11581 ccttcgagca gccccaggcg gtggcccggg ccctggggaa cgggggccgc ctggtcaagg
    11641 acctgggcac ggccaagctg ggcggcctgg acatggcccc ccgggagatc cagggcatcc
    11701 gggagatcgt cttcgtggcc atgggcacgg ccctgcacgc cgcccagatc ggcgcccgga
    11761 tcgtggagac ctgggcccgg atcccctccc gggcggagga cgccagcgag ctccggtccg
    11821 ccaaccccat cgtccaccgg gacgccctgt acgtggcggt cagccagtcc ggggaaaccg
    11881 cggacaccct ggccgcggtg cgggagatcc agatgaaggg cggccggacc atcgggatcg
    11941 tgaacggggt cgggtccacc ctggcccgac tctgcgggag cggaatctac gtgcacgccg
    12001 gacccgaaca ctccgtggcg gccaccaaga gctggagcgc ccagatcgtc tgctgcgccc
    12061 tcctggccct taccgtcggc cgttcccgct acctctccct gggggagggt cgggaggccg
    12121 tagaggagct cgaggccctt cccggcaaaa tcgaggaggc cctggccctg aatgccaggg
    12181 tggacgagct tgcgaagcgt tggagcgggg cccggagcac cctcttcctg gggcggggtg
    12241 tgctctatcc cgccgccctg gagggagccc tgaaactcaa ggagatctcc tacgtccacg
    12301 cggagggatt cgcgggcggg gccctcaagc acggccccct ggccctggtc agcccggagg
    12361 taccgacctt cgccctcctg gcggacgggg agaccttcga ccggaccctc tccaacatgt
    12421 ccgaggtccg ggcgcgcagg ggtccggtca tcgcggttac gaaccgggag gatccccgcc
    12481 tggcggacgc cgcggacgag atcctggtgg tgccgcggtc cctggaggtg ctcgcccccg
    12541 tcctggagac gatccccctc cagctctacg cccgggccgt ggcctccgag ctgggccggg
    12601 acgtggaccg gccccggaac ctggccaaga gcgtcaccac cgagtgagga cgacgcccgc
    12661 gacccggtac gagattcgcg gaactgcgct ccggtaaaat tgacttttac gcgatttttt
    12721 tataaaatgt ccttaccata tgagcaattc cccggccaca gagttcaagg tggatcagag
    12781 ggtcgtctac cccagccagg gtgtgggacg gatcacctcc atcacggaaa agaagttcaa
    12841 ggacgtctcc gtcctgtact acatcatcta cctggaattc tccgatatga ccgtgatggt
    12901 cccggtggac aaggcccgcg acctgggcat ccgccccatc gtgcccaagg acgaggccct
    12961 gaaagccctg aacttcatcg ccgaggaata cgcccccata ccctcggatt ggaagctccg
    13021 gtaccagatg aacctggacc tcctcaagaa gggctccgtc atggacatcg cgtccgtggt
    13081 gcgttccctc taccacaggt ccaagatcaa ggagctgccg atcctggagc gcaaactcta
    13141 cgattccgcc ctcaacctgc tgcaggacga ggtctccttc tccctggcca agcccaagga
    13201 cgatatcgcc gagatcatac gccagaagct ggaaggctga gcccctaggg tcttcgggcc
    13261 gtcctgcgga tggagtcgaa gaggggagcg aaccgcgatt cctcacggta gaacacgagc
    13321 ggatacccca ggggcgcctc caggctgacc gggccgcccc ggaaggtccg gctggtccag
    13381 aaatggaccc acctgtccgg gggaagccac gagtccttga gttcctggcc cttgcgaaac
    13441 accgggcaga ccagcaggtc ccggccgtac aggtattggt gatccaggaa gcggacttcc
    13501 gggtcctcct cgtaatgcgt ccacagatgt cggatcgggg gcagccccgc ctccgagcac
    13561 tcccgggcgg ccgccgcgtg gtagggttcc agggccgcga aaatacccgc catccggacg
    13621 aaatgccgga ggcaggcggg gtcggtccag tattggcagt cggaaagggg cttgttcccc
    13681 tcctgggtcc ggaagaccgg ggaaaacgcg gccatctcga accatcgctc caggcattcg
    13741 ggcttgcggg ccccgaaggc tccggaaacg cagccccccg cgtcgaaatg ccagaacccg
    13801 gccccggaca gacccgcgga caggcccgcg ggcaccacgc tggacaggcc cgcggcgtcg
    13861 ctgaaatccg aaagctggac ccccgcccag aatcccgacg ccagggtcga ggaccccgcc
    13921 cagcccgatc gctggaaaaa ggcgatctcg cccgaggcgc ccgcctcctc gatcgcctcc
    13981 cggcccgccc gggcccagag cacgggccat aggttgtgga ggtcctgggc cttctcgccg
    14041 gaatgcagga ccgcgtcgtc cgggagacat tccccgaggt cggccagcca gcccgacatg
    14101 cccgggtcga tcatccggga gcggagcacg tccttgaccc atcggaacgc ccgggggttc
    14161 gtcaggtccg gcatgaccgc cgtttgggac ccggccccga accggtactc cccgcccccg
    14221 ggcgagcgga ccaggagccc ccgctcccgg gcctcggcgc acaggtttcc ctcggaagcc
    14281 aggaagggat tcacgtagcc caggaaccgc accccttccg aacgcaggga ggcgatgaat
    14341 ccggggaggt ccggatagag gtccgggtcc gcctcccagt tccaacgggg ctggagcccc
    14401 tgccgggtgt cccgggcgcc gcaccagtcc cggacccaga ccgcggcgac cggcgccccg
    14461 gccgtccgtg cggcgtccag tttccggagc acctcccccg ccccgccccc cacgccgagg
    14521 atcgcgccct tccgggtcca gggagggatc ggcggcggac gccccacgcg gcaggccagg
    14581 gaggagaccg tttccgcggc gctgcccccc cggcccaggg aaatctcccg gggggtcttc
    14641 cggaattcca ggaccgtctg ccgggaagag cggaagtcga agcgggcgaa ggaatccgtg
    14701 tccgcgaata cccagtatcg atcggtggag acgaagcacg gaaggggcag gtaggtcgag
    14761 cggatccgcc gggggttccg cttgaggttg gcgggtatcg gaaggggaca gagctcccgg
    14821 gctccccccc gatcctccac ccagagcggc acggaccggc ccttgaggtc cagcgggccg
    14881 aaccgttccc cgcatccgag gatggcctcc ccgggtacgg cggccaggcg gaaccggaac
    14941 cggtcgtacc ggtccctcgg ccgctcgagg gtcagccgga ggattccgtc cgcgtgccgg
    15001 gcggacagcc gaagcctgcc ttcgaaatcg atgaccgcgg atccgggtgc cgcttcctcg
    15061 gcccggaagc ggcggagggg ggtccacttg atcccgcctt cccagggagc ggccccgcgc
    15121 ttcccgccct cggcggtccg gctcaccccc agctccgcgc agggcctgcg ttcggaatgg
    15181 gagaagaccc ggtgcccgtc cacggagagg gtgaagccgc ccggttcggc atccagggcg
    15241 atcaacggag cccgtccctc ccgcggcatc actgcgcgaa cagtcggacc atttccgggt
    15301 tgtggaagag ctcggcgtac ttgcgggcgg agaacccgag cttgtccttc agatccggat
    15361 cggccccgta ggacaggagc ttcctgcaca ggggcacgtc gttccgtcca accgcgatga
    15421 ccagggcggt ctgcccgtcc ttgctggaca ggttgggatt ggctccgcgc ttcaggaggt
    15481 agtccacggc gtccgcgctt cccgcggccg cggcgtccat gagggcggag tagccgcggt
    15541 cctcgctctg caggtccagg gccgcgccgt gctccaccag gagctccgcg acggcctggt
    15601 gcttgctcct cagtgccagg cccaggaggg tcacccccgc ccggtccctc atgtccggta
    15661 tcatcccggc ccgaaggaag agttccaccg ccttgacgtc cccctggcga cagcattccg
    15721 ccagggactc ggacctgtag ggaatcccca actccagaag ggaagcccgg gcccggctgc
    15781 ggccttcccg caccagccat tcggcccgct cggtctcgta gtaggcggcc gcgcgatcca
    15841 gggaatccag gacggtgacc cccgtcaggt atccggggat ccggagggag gcgtcctccc
    15901 ggtacacggc caggcgcttg tcgttgccca ggcaccagcc cccggtgaag gcgaaccagg
    15961 tggaatggac gctttccccg gagaccgcca ggaggatgtg ggaagccttg cggatccgga
    16021 attccagggg cttgcgggac tcgtcctccc aggaggggcc cagcttcagg gcataggcgg
    16081 agatgcccgc cttgcccaga ttcttgagaa gagccagggc ggtctgggag tccttcgatc
    16141 caagcagaac ggctacttcc aaggctcggc actcctgggt gacgggatcg accggacggc
    16201 gagcctcccc gggagcgccg ggggactccc ttagttcttc ggcaggttcg gggcgaatcc
    16261 ttagcgggac ggtcggcgat gcccgggaga cctcagagcc cccgttcccg gagggccgcc
    16321 cggttgctct ccgcctttgc cttcgacagg gtgttgaggc ttcggaagac caggaccccg
    16381 gccacgagga atagggccgg aagcagggcc gtatgcaccc ggatccccgc gacggcggcc
    16441 ggggaacggg gatcccgggc gaagcccgtg gccgcgtgga ccgcccagaa actcagggcc
    16501 tggaccgcgt aggaaagccg ccccgcgaag gcccggaacc ccaggtagac cccatcctcc
    16561 cgcttcccgg tgcggaccac gatcccgtcc accacgtccg ccagggcggg cccgatgagg
    16621 agccagaatc cgccgaaggc ggtcccccag aagaacatgg ccgcgacgaa ggccccgtag
    16681 gtccgggccg ccagaagggg aaggcagaac acggccagga ccagggcggc ggcctgcagg
    16741 agtccctgga cgtccccccg cctgcgcagg aggaccgtcc agatcggcac cgcggccagg
    16801 gccccgagga gcatccccgc gaagatgaag gtcgtgcttc ggccgcccag gacgtaatcc
    16861 cccacgtagt ggatggaggc ggtcatcgag atggtcgcgg actggtagaa gaagaagagc
    16921 aggatgaagg ccaggaaatc ccggtccccc agggcctccc ggaggcgggc gaagaacccg
    16981 ggttcctcgg acgtgcgggc cgtctgctcc aggtaacggc ggatcatgga gggagtctcc
    17041 cggacccccg ggaggaggag cagggcggac accagggcga cggcggcgaa gatccaaccg
    17101 ttcgccgtgt aggaggcggg atcgccgtac cggatcacca gggtgggaag gatggctccc
    17161 ccggcgatgc ccaggacccc caccaccgtc gctacacccg cggccttggc ccgctccgcg
    17221 gtgccccgga accggtccgg gaatacggac tggtagttca gctcccagac ggaatagagc
    17281 ccgtcgtaca ggcacaccga gaacacgagc cagccgaaga ggaggcccgg gtgggttccg
    17341 ccggccgggg atcccggggg ggcgaagatc agcacgaagg atagggcgca cagcaccgtg
    17401 cccgcggcta tccaggggaa gcgccttccc agccgggccg cccagggtgt gggccgggcg
    17461 gtgaggtacc cgatcagggg atcgttcacg gcgttccaca gggagtagac gatcaccccc
    17521 gcggccacga ggcccgcgga caggcccagc tcggtctcgt agtacttgaa caccagggcc
    17581 gcgaatcctc ccgtgaggaa ctccgccagg aacttgccca taccgtagct ggacatggtc
    17641 gggaaacccg cctcctgtgc acccatcgcc tcggttttgt ccgacatagt ggctccttcg
    17701 gatatggaga gtaatgcccc gggggcgggg gaacaaggtc cgcaccccgg atgggaattt
    17761 ttttcccggg tccgcgggtg ttcctttcct gcggatgccg gcgcccgggg tcccgacgga
    17821 cccgctcccg gcgaaaaccc gctaattaag ctccgggaac cgggaaaccg ccgcttggga
    17881 cggttggcac gattgttgct atatagtgtt catcctatat ccgggaacaa tcccggacat
    17941 cggaaaacca agacattcgg aggatcgata tgaacaggaa acgaataacc gcgcttctcg
    18001 ccctcgccac cctcacggca gccgtcttcg cggcaagcgc cgcctggggc tccgccgggg
    18061 ccgccgcagt ccccgcgggg gaactcaccc tcgagtccat gctggcatac gccatccagg
    18121 acgagtacct ggcccgggcg gaatacatcg ccatacagca gaggtacgga atccagcgcc
    18181 ccttctcgaa catccagcgc tccgaggaga accacatcgc ctggctggtc gatgcgtaca
    18241 acacccatgg gctcccggta ccgaaggacg aggcgggccg ctacgtcagg atccccgcat
    18301 ccctcaagga cgccttccag accggagtgg acgccgaggt cgccaacatc gcgatgtacg
    18361 accggttcct ggccaccccg ctcctggccc ggccggagaa cgccgccctg cgggacctct
    18421 tcacccggct ccgggacgcg tcccaaaacc acctgcaggc cttccgcacg ggcttgtccc
    18481 ggtactgata ctacagagta ccctcccggc cgcggtgccg ccgcggccgc cgcccccggc
    18541 gcgcagccgc ggggcggttt ttattccccc ttgcccgccc ggagccaggg acagagccgg
    18601 cgttccgcca ggtccgcaag gccgaagagg cccagggcga gcagggagag ggcggcgatc
    18661 cccgcgaagg tcgtgcggta gtccgccatg gcgtaggcgt tcatgatgag gtagcccgcg
    18721 ccgtaccggg tggcgtagtt ctccgcgaag aagagggccg cgacgcccat gcccaggccc
    18781 agacgaaggg acgtgaagat ccgggggagg accgcgggga tgtagagatg ccgcagaagc
    18841 tccgagcgtc ccatccccag ggaacgggcg gccaggaaga gccgtccggg gatctccgcg
    18901 atcccgtccc gcacggacac gagggtctgg aacacggcca ccgcggcgac gaacaggatc
    18961 ttggacaggt ccccgatccc gaagaggatc atgaacaccg gcaggaaggc gaccttcgga
    19021 accggatgga ggatccgcac cgcggagccc agcaggaact ccgcccgtcg gctggccccc
    19081 agggctatgc cgagcgcgga tccgagcagg atctggaggg ccgtggccgc caggacccgg
    19141 gccagactgg cggccaggtg gacggccagg tcccgccggg ccaggtcggc cagggtcgcg
    19201 aaggtttccc ccggggaggg gaccgcgctg gtccccaagc ccaggtggag gacctgccag
    19261 gccgccagga ccaagagcgc cccggggagg acggacgccg ggaagccccg gcgcgcggga
    19321 tcggaccggc cggaggggcc cgggccgggg agccccgggc gcgtcacggc tccccctcca
    19381 gcaggcgacg gacgcccagg cagaattcgt agtattccag ccgctcccgc aaggacggat
    19441 ccccgaacag ggggttgtcc aggacggccc ggatgcgccc ccgctccatg accgccacgg
    19501 aacttcccag gaagaccgcc tcctcgatgt cgtgggtcac caggaccacc gtcaggtttt
    19561 tcggcgaatc ggagtggctg cacaggccca ggacggcgcg ctggaaagat tccttggaaa
    19621 gggcgtccag ggagctggag ggctcgtcca ggaggaggag atccggctcc gcggccaggg
    19681 cccgggcgat ggcggtccgc tgacgctccc ccccggacag ctccgcgggg tacttgtccg
    19741 ctccgtgacc cacgccgaaa tgctccagga gggacagggc ccggtcccgg tcggcgccgc
    19801 gggaacgccg gtcctgggct tccttaccgg cccgggaccc cccgcggcag ccgagctcca
    19861 cgttctgcag tgcggtcttc caggggaaga ggccgtagtc ctggaacacc agggcggtac
    19921 cggacctttc ggcggggggg cggtcgtcca cgtgaaggga gccgccggcg gccggtacca
    19981 ggcccgcgat ggcgtacagg agggtggact tgccgcagcc ggaagggccc acgacggcca
    20041 gggaggaccc ccggggcaag gataggtcga ggccgtccag ggcccggtcg aaggccggtc
    20101 ccgcggaccg ccccgcccgt cgcccgcggg gatagtccac ggtcaggccg cgcgcctcga
    20161 tcatccggat tccgcctccg gcctcagcgg gacgcgtcca ggaggtatcg tccccagtcc
    20221 agggtcacgg gcttgccctg gaggccctcg atccaggcgg agaccttgtc caggtacccg
    20281 cggctcggaa gccgggtcgg ccggtactcc ggcagggcca tcaggtcacg gacccgggga
    20341 tccaggcgga gccgctcgac caggatgtcc cgggccaggg cggggtccgc ccggatgtcc
    20401 gcgaccgccc ggtccacggc ccggttgaag gcggcgatcg cctcccgctt ggccccggcg
    20461 gcccgggcgg tgaagaccat cacgtccgga tagaagtcgt ccttgttctc gaagacccgc
    20521 ttctccagac ccgccagctg gcccatggag gcgatgggtt cgggaaggca ggccatgtcc
    20581 accttgcccc gcttgatcat ctccaggcgg gcggggatgt ccgtgatgaa gaccttctcg
    20641 atctcgtacc ggcccgcgag ccacgcgtcc gccaggtagt tggagacgct cacctccatc
    20701 atggccaccg aggccttccg gccctccggg aagcccgggc gcaccaggaa ggggaaggac
    20761 ccgtccgtgg agctcgtgat ccggaggtcg aagccgccct ggacgttgta gacgaaggcg
    20821 accaggtcgc tcatggaccc gtccaccgcc ccggactgta aggccacctg tcgctccgtg
    20881 gcgttcggga agacgaccag ctcgacggaa accccttccc gggcgaagta cccgcgcttc
    20941 tcggccagca gggccggggc ggagtccacg gcgggcatga gcccgacccg gagctccggg
    21001 cccgccgcgg acaggggaag ggccgccggg gcgagcagga aaagggcggc cgagaggcgg
    21061 aaggaccggc cggagcgggg cttggaccgt atggaacgcg gcccccgcgg ggccggaagg
    21121 gcagcgggca tgttcgagac ctcctgggtt ccgcgcgctc cgggggctgg agggagcgcc
    21181 cttcctatgc taccctcgcc ggcggggccg gtacaatacc cggcccgatc cttcgaggac
    21241 gaggaaggtg atcccgtcat ggaattgctc gaatgcggcc ttccggccct gatccgggac
    21301 ctctggatcg cggcccgccc cttctccctg agcctggccc tgtacgccac gagcgtgccc
    21361 ctggccctgg ccttccgggt ggggatcctg ccgggcgacc ggccgtgggt cggggccttc
    21421 cgggctctgc tcgtgaccct ctcgggcctg gccgtccagg ccgccacgaa cctcatcaac
    21481 gactacttcg agagcggcat caagggcgcc ccggaagccg agcggaagta ccggttcctg
    21541 ggccgggacc ggggagcctt cgagatcctg gttttcctcc tggccctggc ctgcttcggg
    21601 ttcacggccc tggcgggcat ctacctggtc ctgaccgtca ccccggtact cctgtggatc
    21661 ggcgccctgg gcctggccgg gggctattgc tataccgggg agcccgtcgt ctacaagcgc
    21721 ttcgggctgg gggcggtcct gtccttcgcc ctcctgggac ccctgatgaa ctatggggcc
    21781 tggctggggg tcggagggac gccttcctgg ctgcccgtgc tggtggccct gccgacttcg
    21841 ctgttgattc ccgccatgat gctgtccaac gagatccggg acgtggagcg ggacgccgag
    21901 ctcggcatcc gtaccctgac cgtccggatc gggcggaagg cggggcaggc cgtctacgac
    21961 ggcttgctgc tggcgacctt cgggtgggcc gctgccctcg tgctcctcga cctggcgcct
    22021 gccgcgaccc tggcctcctg ggccgccctg cccctggcgg tccgggcccg ggtccgggtg
    22081 gccgaacgcc gccgggatgc cattccggcg accaacctcc tgcatctggt gttcggggcg
    22141 ctgtatatcg ggggattgtt atggtagccg gtggactggg gactgtggac ggaattgtct
    22201 accgtccgcg acggccggct aggctatagc gaatgattcg gccgaggtcg gaacacactt
    22261 ccgactactg tccaccgttc actgtccacc gtacaccagc ttccacgcct tcccgactcc
    22321 cgtaggggtc tcctcgagct cccggaactc gtagggctgg ccggccacgg cctgccggac
    22381 cgcgtcggtg gccaggatct cccccgcctt ggccacgtcc tccccgagct tgctggaggc
    22441 gttgacctcc cggccgaaga cgtcggcgtc cccgatccgg agcatcttcc cgtacccgag
    22501 gcccacgcag agcaggacct tctcctcgtc cgaacgggta cggttgtagg cgtcgcaggc
    22561 ggcctgcatc tccagggccg cgtgcagggc cttggccggg ttgcggaaga tcaccagcat
    22621 gctgtccccc tcggtcttga ggaggatgcc gtcgttgtcc tcaaggacgg gcaccaggat
    22681 gcgctcgctc tcgtagatgg tctgcaggaa gtggatgatc ccgaacttgg ccacgttccg
    22741 ggaaaacccc gagaggtccg tgaacatcac cgcccagtcc tccccgaaga ggtcccagat
    22801 ccggccgtcg atcctctccc ggtcggaacc cggcttcagg cgctcctcga tcaggcgttc
    22861 caggcgttcc tccgaggcgg tggatccgat acggcggcgt acgggcatgg gggacctcct
    22921 gagaggagtg taatcggtaa acggtgaaca gtaaacggga atgacttcgg tccgcgttgg
    22981 gtggcaacgc taaatcgtat gaaccggccg cgactctggg gtcccttcgg tccgcagtcc
    23041 actgtccgtc cttacggcac caccttcacg gcccggtacc ccgcgtggtt cacggctccc
    23101 tcgagctggg ggcctccgaa accttcgccc tccaccgtcg cctcgccctt gaggaggtcc
    23161 acgtccgcct tggtcacccc ggggactccc tccagggcct tcttgacccg catgacgcag
    23221 tgcccgcagc tcatgccctc tatggtcacg atcttcttca cgaatcgctc cttcggggga
    23281 tctaatatac cccccagggg tatattacta cgaactcggg aaaaagaaaa gcgccgagct
    23341 gccccggcgc acgctttctc gaatcaccac ggagggcacg gagaggagag gatccggaat
    23401 acggggattc cagaaacctc gacgaagccc gcagggcttc gttgcccgac ggggcggctt
    23461 atgccgcccc gtcggtcagc ctctgtaaca caggatcggg caccgggcgg cggccacctc
    23521 gtccaggcgt cccacggggg tgacgtgggg tgccgagtgc agcagctcgg gctcggtccg
    23581 ggcctcctcg gcgatcttca gcagggcgtc cgcgaaagcg tccagggtct cccgggtctc
    23641 ggtctccgtg ggctcgatca tcagagcctc ggggacgatg agggggaagt agatggtcgg
    23701 cggatggaag ccgtagtcga tgagacgctt ggcgatgtcc agggtgtgca ccccctccac
    23761 gaagttgccc atggccacga actcgtgcat gcagccccgg ccgtacttca gggggtagac
    23821 cttctcgacc ttggccttga ggtagttggc gttgagcacc gcggtctccg ccaggcgccg
    23881 gagccccggg gccccgagca tgcggatgta ggtgtacgcc cgcaccagga ccccgaagtt
    23941 gccgtggaag gccttgaggc ggccgatgcc ggccttgggc atggccatgt cgaaacgccc
    24001 gtccttcttg accgcgatcg ggcccggcag gtagtccgcc aggcgctccc ccgcggcgac
    24061 cattccggat cccgggcccc cgcccccgtg gggggtggag aaggtcttgt ggaggttgaa
    24121 gtgcatgacg tcgatgcccg cgtccccggg ccggaagatc ccggtgatgg cgttcaggtt
    24181 ggccccgtcg ccgtacacga ggccccccgc gtcgtggacg agcttcgcga tcttctcgat
    24241 gttccgctcg aagaggccca gggtgctggg attcgtgatc atgatccccg ccacggtgtc
    24301 gtccagggcc gccgccaggg cggccagatc cacgccgccg tcggcgtcgc tggggatcgt
    24361 gacggtctcg aagccgacca tggagcaggt ggcggggttc gtgccgtggg ccgagtcggg
    24421 gatcagcatc ttcacgcgct tcaggtcgcc ccggtcccgg tgccacttgc ggatcatcag
    24481 gacccccgcc agctccccgt gggcccccgc ggcgggctgg aggctggcgg ccttgaagcc
    24541 gccgatctcg accagcatgg cctggagccg gtacatcagc tccagggcgc cctgggagaa
    24601 ctccgcaccc gccagggggt gggtggtgcg ccagcccggg agggaggcgg cggcctcgtt
    24661 ggccttgggg ttgtacttca tggtgcagga gcccaggggg tagaactccg tgtcgatgga
    24721 gtagttcttc tgggacagcc gggtgaagtg ccggaccgcg gtcagctcgt cgatctccgg
    24781 gaggggaagg tcgtcccgca ggagcccctc gggcagggcg gtctcgggga cgtccggttc
    24841 cgggagggcg gcgccgcagc ggccgggggc ggagagctcg taggcgaggg gttcgggtcg
    24901 aaggttcact tggaggcctc cttgagggac gcggccagga ggtcgatctg ctccttggtg
    24961 ttcatctcgg tcacgcagac cagaagctcg tgggttcggt ccggccaata ccgggagagg
    25021 gggagtcccg gcaccacgca gcgccgggag agcttgtcgt agatcgactg ggcgggcacc
    25081 ggagtcttga ccaggaacag tatcatgaaa gcgatcgcga acctcaacac
//
LOCUS       DAOTEI010000033        24826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1322_length_24826_cov_5.693109, whole genome shotgun sequence.
ACCESSION   DAOTEI010000033 DAOTEI010000000
VERSION     DAOTEI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 24826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24826
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1322_length_24826_cov_5.693109"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="P5313_04970"
     CDS             complement(<1..781)
                     /locus_tag="P5313_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79748.1"
                     /translation="MAAIIQVEGVSKEYRLGVIGNGSLYKDFQSWMAKIRGKEDPWTK
                     IGAVSKFGKRDRFWALKDVSFEVEAGDRLGIIGRNGAGKSTLLKILSRITAPTAGTIR
                     MRGRLASLLEVGTGFHQELTGRENIYLNGAILGMTKADIDRNFDEIVAFSEIEKFLDT
                     PAKRYSSGMYIRLAFAVAAHLETDILLIDDIQFIAGKESTQEEFFHTFNALYGQDKQI
                     VMTSDRSPKAMSTLEERLRSRFEWGLTVDMQAPDYETRLAIL"
     gene            complement(791..1654)
                     /locus_tag="P5313_04975"
     CDS             complement(791..1654)
                     /locus_tag="P5313_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79749.1"
                     /translation="MKAAGNASTGDWDIVITAKDKLLSFDFKELFQYRDLIKLLIRRD
                     FVAVYKQTILGPLWFLFHPIMSTIVYMFVFGNIAKIGTDNIPRPLFYFSGTMLWTFFA
                     TTLQKSSDTFIVNAPIYGKVYFPRFSMPIVYAVNSFFTLAIQFVCLLAIYVVYLVSGY
                     SFHPSWWLLATPILVLQTALLSLGIGIMVSALTTRYRDFRNLIHFGLQMMMYVTPVVY
                     PMSEVPARLKPLVMLNPLTPVMELFRHALLGGGEGNLTLWAVSLVETAAVFWLGMRVF
                     NHNERTFIDVV"
     gene            complement(1731..2450)
                     /locus_tag="P5313_04980"
     CDS             complement(1731..2450)
                     /locus_tag="P5313_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79750.1"
                     /translation="MLGILLLAAAAPGLSAQPQPFRNLSSEEAAAVSAGKSVFRRPSG
                     WKDLSVPVAAPFAKDIEDTVRKLGANYVGEVVLVLPKASNPDLLVILARNLADVESHV
                     GIPYWSRRYGKNFDLFTWVKVLERTGGGSEGGLTSEQYMKPFDPYRARYSWSLRGDRL
                     SFLSTNLSHLSYDGKKAVSPGEMVWRLEAYAEGDRWVLYGIGAVKAFDMLGLLRDRLS
                     ESFMGRIEAFFGYMYRKGVEL"
     gene            complement(2867..3217)
                     /locus_tag="P5313_04985"
     CDS             complement(2867..3217)
                     /locus_tag="P5313_04985"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY79751.1"
                     /translation="MNGDDSSRILEADETLGDIVDILVLAVNPDRIFLFGSRSRESEV
                     TGSDYDLLIVKNGVSNEREVSRAAYRSLLDHGSAVPVDILVADEERLEIARGKIGNVL
                     AGALVDGVKIYERA"
     gene            complement(3340..3906)
                     /locus_tag="P5313_04990"
     CDS             complement(3340..3906)
                     /locus_tag="P5313_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRY79752.1"
                     /translation="MQTDTELAVLENLYGSGKAESSRAPTQRALARTAGLSLGMTNVL
                     LKRFTEKGWVTVKRVNARNLHYALTPEGVQEIARRTYRYLKRTARSTALYRDLLEEHV
                     LAAKREGVRTLVLAGPSDLDFILEYVCERHGLVFLKTADPVRARALDGPEVRTVYAEG
                     VPEEARLPGSRGLADILAGRASGAEGGE"
     gene            4275..4814
                     /locus_tag="P5313_04995"
     CDS             4275..4814
                     /locus_tag="P5313_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRY79753.1"
                     /translation="MTFQPPPNVSVLPYKGMIPRIHPSVFLCDGVRIIGDVEIGEGSS
                     VWFNTVIRGDVNSIRIGARVNVQDLCMLHVTKRKFSLSIEDEASVGHSVSLHGCVLKS
                     GCLIGIGAIVLDGAVVGEGALIAAGTLVREGVEVPPNTLFAGVPGRVIRELSGEDRSR
                     VAATAPHYVEYAALYRSGS"
     gene            5053..6441
                     /locus_tag="P5313_05000"
     CDS             5053..6441
                     /locus_tag="P5313_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HRY79754.1"
                     /translation="MSSKVMKVPHTYVILFALIVVAAIGSYIIPAGEFDRAKDPNSGR
                     TVVVPGTFHKVEQNPLSFFDTFISVQKGSIDAADVVFFIFFVYASFYIVLQTGALHSF
                     IGWLLRVLKGKEIIIIPIFMYIFALGGSVFGMYEETFGFIPLFVGLAIAMGYDALVGM
                     SIVSLAVAMGFAAAFMNPFTVGVAQKVAELPLFSGMGFRIIVWFVHVTMAVVYTLLYA
                     IKVKKDPSKSILKGMDMGSLALNHDELVQKKLTGRDKLILLWVAVIVGLLVWGVIVKG
                     WYFDELAGLFIIMGVAAGFMAGWGPSKIAVTFLEGAKDIVFGALVVGLSRGILIVMRE
                     AKIIDTVINAMATPLSYLPRWLAAEGMLIVQTLINFFIPSGSGQAATTMPIMAPLSDL
                     LGITRQTAVLAFQFGDGFSNVLWPTTALPVICSIAKVPIDKWWKYFVPFFGWLLVAQM
                     IFMAIAVAINWQ"
     gene            6525..7868
                     /locus_tag="P5313_05005"
     CDS             6525..7868
                     /locus_tag="P5313_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY79755.1"
                     /translation="MSLDILVRGGTALDPAAGTERALNVGIEGGRIAFLGPEAPSAAR
                     VIDAAGLLVSPGFIDTHMHDEELEDPDTIQLALLRQGVTTAIAGNCGSGPLLRDIRAA
                     RPAPWLNLGYQTGHRYLRREVGIDDEYRPATEAEIARMEALLRNELDLGSLGLSFGLE
                     YAPNTSPREIDALCAVAARYPERVVSSHIRYDGPECLKAVQEMIDLARRHRVRVQISH
                     IGSMTAFGHSAEALRMTDRARSEGLDVTMDCYPYAAFCTYIGSAVFDPGFEERWKKGL
                     ESLEAASGKYKGGRLTPASYEDLRANDPHALIIAHVMNEAEIRLCLTHPKCAVASDGV
                     LHQGQGHPRAAGTFPRALRWLREAGLSWPEAVRHATSLPASMAFLSSGVLAIGAPADL
                     VLFSPERLKDKATFQDQLAPPEGIEYVIVNGKVALEKGEVTRPAVGRLLTRPPMK"
     gene            7890..9074
                     /locus_tag="P5313_05010"
     CDS             7890..9074
                     /locus_tag="P5313_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRY79756.1"
                     /translation="MDYKQFLPELDAVVDRLAPRAISLSDAMAADPEISEQEVRSSKA
                     HADFLRDAGFRVEHPFFGIPTAYNATVSSGTGGKVALLAEYDALPEIGHACGHNVHGA
                     MSLLAGAALAPLMGKIGGELWVTGTPAEESNGAKILMSAKGLFDDRDLALMIHSGGEK
                     SYVRYRCLAMDSFEFRFKGKTAHAAASPWEGRNALNGAQLFFHALDMLRQHVRPDVRI
                     HGIYTQGGAACNIVPEAATVRFYFRAARRAYLDTDVKKVLEAAKGCAQATDTEVEWSN
                     FELSFDEMRPNDAAETMMEEVFTEAGAPFGPSPGPQGSSDVGNVSMRCPALQPTLSII
                     DKPYTWHTREFAASTTGPLAHEALVKGARILARASLRALLDPDLRARMRADFEAERNR
                     HD"
     gene            9067..9525
                     /locus_tag="P5313_05015"
     CDS             9067..9525
                     /locus_tag="P5313_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRY79757.1"
                     /translation="MTRSDRARELHASGSNCAQSVLVAFAGDLGVDPKQAHRLATTLG
                     AGLGRRQLLCGAINGGALALGAAFGNEEGSDLATKELCYEIVRDYLRRVEEIAPSTEC
                     RVLLGVDISTAEGREESKRLGLTAKVCDPLIGKCAEIVEEILAERGRIAR"
     gene            9522..10526
                     /locus_tag="P5313_05020"
     CDS             9522..10526
                     /locus_tag="P5313_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79758.1"
                     /translation="MKSEAGPPWVRYRRLIDLEGVPPAAPESRAALADLLAAPEVRVL
                     LSDVASWPGTVLASHRSPSQTFHSLGFLAELLAGSWLGDGVAPAIGAAVARIRERTGP
                     DGIPRLPMTYPEHFGGSGVETWAWALCDAPILLRARVRFGGASEPLVRRGIRSLVALS
                     EPFGWPCRVAPELGSFRGPGKKSDPCPFATLILLELLASLREEAPADPEARDLAEGPE
                     ADAGIEALLDSWARSAERHPYMFYMGTDFRKLKAPGLWYDILHVSDVLSRFPKARGDP
                     RFLDMLGVLRSKARPDGTFVPESAYQPYKAWDFGQKKEASPWIGLAIARIEARMGGRD
                     "
     gene            complement(10523..11458)
                     /locus_tag="P5313_05025"
     CDS             complement(10523..11458)
                     /locus_tag="P5313_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY79759.1"
                     /translation="MSASTSSRDQTLGLAALAVCALLWSLAGLFIKIIDWNPMAIAGA
                     RSAIAGLFLLAWIRKPRITFSFPQLGAALSSAATMLLFVYANKATTSANAILLQYGAP
                     IYTAILGIFLLKEKPRLEHFAAFLAVAAGMVLFFLGDLGGGSLLGDLAGVASGVTFAM
                     YYVFLRMQKDGSPLESNLLAHGFTAVIALVWACFLPAPKVTWASGAAIVTLGVFQVGL
                     AAVFLSFGIKRVTAVQGILVAAIEPIANPVWVFLATGEAPGPNSIIGGAVIIAAVLAS
                     SVVSMRRDALAAERALTSGSGTPMMVDSGNPRTAD"
     gene            complement(11690..13051)
                     /locus_tag="P5313_05030"
     CDS             complement(11690..13051)
                     /locus_tag="P5313_05030"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRY79760.1"
                     /translation="MSRAAAGRPEPLSLREARALALAAGAGFPPAFPAGTEGVARAVE
                     ALGFVQIDTISVVRRAHEHILWSRVPDPGFSAVPALEGVGGGERRLVEYWAHAAAYLP
                     VEEWRYCLPRMERIRAEGHEWFRAEPEVVSRVRDRVAAEGPLSAKDFQESLSGPRGWW
                     DWKPAKVALEYLFHSGELACATRRGFQKVYDLAERQIPAAHAGPAPTEAELSARHVER
                     AARSLGIFTKAQAAYQRKDGLAGMDAALDAAVEAGTLVPAAVEGLGPGWYARRDLLGQ
                     TATDPGVDGADGGAGHSSPAVPARPRAFVLSPFDPFLIDRRRTARLWDFDYTLECYVP
                     AAKRAFGYFTLPILYAPGDGGEARFAGLLDAKADRRRRTLVLRRLRLARGGSVAPSGS
                     ADAALNPVKPADLARSVAAELARFAAFQGAEVLELEVLETDDPRLEKAFRREFARVSE
                     ALH"
     gene            complement(13092..14078)
                     /locus_tag="P5313_05035"
     CDS             complement(13092..14078)
                     /locus_tag="P5313_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79761.1"
                     /translation="MSPSRAPAGEKAAGVDPAVLAWLLAGDVSVQYRVRRDLLGRDER
                     ELASLRARIPLEGWGRRFLEARDPGTRMWGGGPYMPKWVSTHYTLMDLLNLGFPGDHP
                     AFREGVELLLERFLGPDGGIYVAKTVRTADVCVSAMVLSLVLYSGIRDERARRILSYL
                     AAHRQPDGGWNCFDVRSAASSFATTLSVLEALEDAGKAGLAPAMELDRLSRGGEEWLL
                     ARRLYFGLRSGRIANPEFPYLSFPCRWKYDVLRALSYFARAGRPFDPRMTDALALLRG
                     KRRKDGTWPVQRKHPGAVHFDMEKTGGPSRWNTLRALVVLGAYGKAAGVEVS"
     gene            complement(14075..15664)
                     /locus_tag="P5313_05040"
     CDS             complement(14075..15664)
                     /locus_tag="P5313_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY79762.1"
                     /translation="MSRKSRSGRNVRPLAAPTAALAVLALTAVLAASCSISRPSPMVG
                     RPPEGELKEELALPAPMAQKSMMMDRAAPPPADGTGTPSSEEYKGLPENPFKLAKDER
                     FSTFSADVDTASYSNIRRFLNEGRLPPPEAVRIEELLNYFDYGWKEPAGAEPIALSAE
                     SAASPWSSGRVLLALGLRTRTVSAANLPPANLVFLVDTSGSMSDPDKLPLVKRAFSLL
                     AETLRPQDRVSLVAYAGSAGLVLPPTPGTEKGKILAALEALESGGSTAGGQGIVLAYD
                     TALQNFRKGGNNRVILATDGDFNVGLSSESELVKLIESYRNRGVFLSVLGFGTGNYKD
                     AKMEALADKGNGNFAYIDSVEEARKVLIEEMGATLLTAAKDVKLQVEFDPAVVKSWRL
                     IGYENRILAKEDFTDDKKDAGEMGAGHRVTALYEIEPVSPAPGAGLGILRVRYKKPDQ
                     DQSAQLELALADARGSLSNASGDLRFAAAVAAYGMILRGSEHRGRADWKLVLDLARGA
                     AGDSDRRAEFVTLAEKASGLK"
     gene            15838..16803
                     /locus_tag="P5313_05045"
     CDS             15838..16803
                     /locus_tag="P5313_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HRY79763.1"
                     /translation="MFQPVQVIFAPTSRCNLACSHCRVRRGPEELSAPEASAFLERCA
                     RGGIERVGFSGGEPFLRPDFLAEVSRTAVASGLYFDRLMTNGDWFADPADLEESLSAL
                     YEAGFDGTFGVSADAFHDQDPGRLAAFLSAVFRIWGRKDCAEILSVRAPDEGPWLEGL
                     RVLAERLGGRLVPRTGEPRGIADAAPRREEAAAGEVLDIPILRFPRSAAGTETGWGED
                     RWFREDFCEGPGNVFYVHPDGSVAVCCGFANENPSLIVGNIRDDDYDSLMESARANPH
                     VRLVYEKGLDARRRELEASGVKFPGMTDDICFFCDHLYRNKLIGD"
     gene            16901..17518
                     /gene="rpsD"
                     /locus_tag="P5313_05050"
     CDS             16901..17518
                     /gene="rpsD"
                     /locus_tag="P5313_05050"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRY79764.1"
                     /translation="MSRTREPKGKIVRALGLNVFGNPKFDRLLDRKPHPPGKPPRYSP
                     RKTSEYADQLRETRKYRTAYGLGRRQLKNLYEKARRAGGSTSAALLVFLEARLSNVVY
                     RLGWAVSRAQARQLVSHGHVAVNGRTLDIPSAALRPGDALELRAPESLARLVRERTAS
                     EGAARLPSWLAREDGGRAARVASAPVPEEASVPGRIGRVVEFFAR"
     gene            17662..18825
                     /locus_tag="P5313_05055"
     CDS             17662..18825
                     /locus_tag="P5313_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79765.1"
                     /translation="MKRIVFFVIAAAMCARLPGVYAQDLSEPDEQSVLQKARDKYGRT
                     AEPSDPSSATDPSDAEDSGRPFSPFVFSFVPGIQFPPFGLYDTSAAFGWIGSGVGNVE
                     GIQASGVFNLARDVEGFQGSGVFNIARDVEGFQGSGVFNIARDVEGFQGSGVFNIARK
                     VEGFQASGVFNIADTVDGGMVAGVFNAAERVSGPMIGLVNVADRLDGVALGLVNIIGN
                     GVNEIGLQYIPAEDMTYLAYRTGTASLYSVYYGGVGSEDWFVDSDSLVVGLGWGHRIR
                     IGDGGLDFEVCAEQELYADRRAAMETAADADDEDAFLALIRPYPSVRAALNIPVLGFK
                     AVLGIETDFDVPAWGSYVPDRLRASSFRTGGWSGDAWGLEFTAWPKVFLGLSF"
     gene            18981..20087
                     /locus_tag="P5313_05060"
     CDS             18981..20087
                     /locus_tag="P5313_05060"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRY79766.1"
                     /translation="MKRLIVIAACLLTVVSAAAAQTLFGEIEYVEGDVTLSRGGKSLG
                     DLNIGDEVQVDDFIKTGPDGAAVIALNRSTGMRGSLSVKPRSAVYVRMSQDSSGSKTT
                     LELVAGQIGSKVAKLSGSPTLQVRTDTAVMGVRGTEFGVSTSVSGNIVIFCTEGAVET
                     SDGESTVQVPAGKAVEKRPAERLRLLPVAVSNPDEFAKRWIADEIEAFRANPARVVAF
                     YEKRYADLSVRFTAALDDLQRKAALRKWIEEDKAGTRVNPRSADTLREKKELVQSFLE
                     LRKILFLFERIYYRLDEAVDLVSGTPGERAELRPGLTAGAFLRQFRDERDRLAKMMAF
                     FRYAERLYALRNEGGAGFPGLGGDDDDFFGTSDF"
     gene            20137..20823
                     /locus_tag="P5313_05065"
     CDS             20137..20823
                     /locus_tag="P5313_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRY79767.1"
                     /translation="MTTVISLGGSIVAPDKPDVPFLRDFLALSTAWLEEDPERRLILV
                     VGGGGPAREWQKAYRETARRADPDAQDWVGIMATRLNAQLVKALFGELCPREVVIDPT
                     AVGIFPGRVLVAAGWKPGFSTDYDSVLLAELFGAGTIVNLSNIAKVYTADPRLDPAAR
                     PIDSIGWAEFRALVGDEWVPGKNVPFDPVASRRAQELGLRVVCAAGRDLPNLRAILEG
                     GPFTGTVIGG"
     gene            complement(20849..21223)
                     /locus_tag="P5313_05070"
     CDS             complement(20849..21223)
                     /locus_tag="P5313_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79768.1"
                     /translation="MNSNSGGGFRPLVLFALALTLASCAGLSYSHTGRIWDLPGTNPV
                     VSDTIAPLRDVTLAVTGSLESGSVTVRVYADSAVRGTLDIVPAASVSERKTFEYAPGL
                     WRFEAEASDGSAGSLYIEIEDR"
     gene            complement(21295..22050)
                     /locus_tag="P5313_05075"
     CDS             complement(21295..22050)
                     /locus_tag="P5313_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79769.1"
                     /translation="MPGPDPGRGGEPDACLFGRLRTGARRRGFALLAAAVLAGCATAP
                     AGRGLPPSVKRFLDRNGWNLAGYESSDLESPAGYFFHDRSYSSPAPPDLVWKAYTALE
                     PSAAWKTSRTRPGVVWDPSGSRLYAAGETAPPFSPGQVLVLDVRILGPFRIPASFRIT
                     RIDEPERLIEFVYLKRNLSNGVQRIRFLERAGPDGSPSTVIVHSTWYRSGRPFRDRSL
                     YGPIHARIIDAFHAGVFRKSGLILDRPVRNAPE"
     gene            complement(22037..23026)
                     /locus_tag="P5313_05080"
     CDS             complement(22037..23026)
                     /locus_tag="P5313_05080"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY79770.1"
                     /translation="MSANLSAFPGRAPRRGRPAARSFLVPVTLAAAVLSACSTAPKPV
                     AEVVARKNQAAEYSKLGDGFLAKRDYASAQRFYEESLRENRAVDNTEGVAISMNSIGR
                     VYLAAGRLDDAEATFREALDYAGPLKNPSFRALALANLGEALYARGDGAAAMEAFQEA
                     LPLAARDERTLAVLLHDRAVVYRDQGEWAAAESDLRRAAGINQRLKRLAEYGANLYLL
                     GNIAHKKGNSEAALDLLRQALEADRKAENSAGIAADLEALAVLSRGLGSREDAFHYYR
                     RAFDVFVTLDSAEAALRCAEALAVLAAELGRPEDVRRYREYAARIRSSLDARP"
     gene            complement(23023..24120)
                     /locus_tag="P5313_05085"
     CDS             complement(23023..24120)
                     /locus_tag="P5313_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRY79771.1"
                     /translation="MKFRIKHADRIVGLFVLVAVGLLCAIVILLGANQRWFAKNYEFK
                     SRFTSGTGIPPGTPILMRGFQIGKIKKITLTEENQVDVVFDIYDTYYPKVRQHSILEL
                     SVSPIGLGSQLLFHPGKGEEVLAEGSYIPAADTAEGQAVIEQELAEIPPRDDTVSRLI
                     AGLNPLLENASRTLVTVNRTLTEVNRALAGQSTGPLGSIVGDVSKAASELPRAVAGAS
                     NLIEDVEGRTDALLTELQGILASVNEVSASLAKAAKEMEDPTGLVPKLLDPKGSLKTF
                     LDDNNALYNKVLGMMGDVERSIRSLREIMGSLSSEMPKIASLLEEGKTTLQKTQDVLE
                     GLANNPLLRKGIPERQEQQSLYRSIREGDFQ"
     gene            complement(24117..>24826)
                     /locus_tag="P5313_05090"
     CDS             complement(24117..>24826)
                     /locus_tag="P5313_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY79772.1"
                     /translation="AAGLVVPDSGSVLFRGASLASFSEDEERYFRSRTSFVFQDSALW
                     ANQTILNNIALPLRVHRHWMGESEIAERIREVLARVGYDEGLALRPAELSAGEQKLVS
                     IARALVLDPELVFLDEPASGLDEDAKERVQEVLDSLRDAGKSVLLVTNDMGWVHRVAD
                     RIVIIRDGRIAAAGLYDEVVASEVPETAGLVARLRARGARARNAPESPVTVVRPAREP
                     SEGERSADNGADEERGS"
BASE COUNT         3969 a         8457 c         8330 g         4070 t
ORIGIN      
        1 gcaaaattgc caggcgggtt tcatagtcgg gcgcctgcat atccaccgtc agcccccatt
       61 cgaagcgcga gcgcaggcgt tcttccaatg tactcatcgc cttgggagag cgatcggagg
      121 tcatcacaat ttgtttgtcc tgtccgtaga gggcgttgaa agtgtggaaa aattcttcct
      181 gtgtggattc tttgccagcg ataaactgga tatcatcgat caaaaggata tccgtttcaa
      241 ggtgagcggc aacagcaaag gccaaacgta tatacatgcc actcgagtag cgcttggctg
      301 gagtatccag gaacttctcg atctcggaaa aggccacgat ctcgtcgaag ttccggtcga
      361 tgtcggcctt ggtcataccc aggatggctc cgttcaggta gatgttctcc cgtcccgtga
      421 gttcctggtg gaagcccgtc ccgacctcca gcaggctggc caggcgcccc cgcatccgga
      481 tcgtcccggc cgtgggcgcg gtgatccggg acaggatctt gaggagcgtg gacttcccgg
      541 ccccgttccg gccgatgatg cccaggcggt ccccggcctc cacttcgaag ctaacgtcct
      601 tcagggccca gaaccggtcc cgcttcccga acttggacac cgccccgatc ttggtccagg
      661 gatcctcctt cccccggatc ttggccatcc agctttggaa gtccttgtag aggcttccgt
      721 ttccgatgac gccgagacgg tattccttgc ttacgccttc cacctggatg atcgcggcca
      781 tgatgctccc ttatacgacg tcgatgaacg tccgctcgtt gtggttgaac acgcgcattc
      841 ccagccagaa caccgccgcc gtctcgacaa ggctgacggc ccacagggtc aggtttcctt
      901 ctccgccgcc cagcagggca tgccggaaca gctccatcac gggggtcagg ggattcagca
      961 taaccagggg tttcagccgg gccgggacct cggacatggg atacacgacg ggcgtgacgt
     1021 acatcatcat ctggagtccg aaatggatca ggttccggaa gtcccggtag cgggtggtca
     1081 gggcggacac catgatgccg atcccaaggc tgagcagggc ggtctgaagg actagaatcg
     1141 gcgtcgccag gagccaccag gacgggtgga aggagtagcc cgacaccagg tagacgacgt
     1201 agatggccag caggcacacg aactggatgg ccagggtgaa gaaactgttc accgcgtaga
     1261 cgatgggcat ggagaaccgc gggaagtaga cctttccgta gatgggggcg ttcacgatga
     1321 aggtgtccga agacttctgc aacgtggtgg caaagaaggt ccagagcatg gtcccggaaa
     1381 aatagaagag aggccggggg atgttgtccg ttccgatctt ggcgatgttc ccgaacacga
     1441 acatgtacac gatcgtggac atgatgggat ggaaaaggaa ccacagaggg cccaggatgg
     1501 tctgcttgta caccgccacg aagtcgcgcc ggatgagcag cttgatcagg tcccggtact
     1561 ggaagagctc cttgaagtcg aaagacaaca gcttgtcctt cgcggttata acgatgtccc
     1621 aatcccccgt cgacgcgttc ccggcagcct tcatttccgc tccttgcggt accgtaagtt
     1681 tttacgcccc atcttgtcta tcttccctca tcctgtccat cctgtcttcc ctatagttcc
     1741 acgcctttcc tatacatgta cccgaagaac gcctcgatgc gccccatgaa ggactccgag
     1801 aggcggtccc gcaggaggcc cagcatgtcg aaggccttga ccgccccgat cccgtagagg
     1861 acccagcggt ccccctcggc gtaggcctcc aggcgccaga ccatctcccc gggagataca
     1921 gccttcttcc cgtcgtagga caggtggctc aggtttgtgg acaggaacga gaggcggtcc
     1981 ccccggaggc tccaggagta ccgggcccgg taggggtcga agggcttcat gtactgctcc
     2041 gaggtgagtc cgccctcgga cccgccgccg gtccgctcca ggaccttcac ccaggtgaag
     2101 aggtcgaagt tcttcccgta ccgccgggac cagtagggga tgcccacgtg gctctccacg
     2161 tccgccaggt tccgggccag gatgaccagc agatccggat tggaggcctt gggcaggacc
     2221 aggaccacct ccccgacgta gttggctccc agcttccgga ccgtatcctc gatatccttg
     2281 gcgaagggag ccgcgaccgg aacggacagg tccttccagc ctgagggccg gcggaagacg
     2341 ctcttcccgg cggagaccgc ggcggcctcc tcggagctca ggttccggaa gggctggggt
     2401 tgggcggata ggcccggcgc ggcggcggcc agaaggagaa tcccgagcat tcgaatcctc
     2461 catggttgtc ggtcctgaat ccgcccagtg cctgatttag ccatgttctt tcctgattat
     2521 accgaaaacc catgaataac agcctccggc gagcctgacc gcttcacgga gttcatcctc
     2581 ggataccgga gtgtactcgc cgggataccg catttgaacg gcatagtcat tgaggataac
     2641 cgtttcccgg atgctctccg gaattgccgc gtacttcccg acggtatgta gcagcgaaac
     2701 ctaatcatgg gttctcgggg gtctctcccc gaagaatatc agcaaagcct tcaggccttt
     2761 ttcgacagat tgctgcgcct ggaagcaaag atcctcatat atgatctcat cggccttctg
     2821 cgttcttgcc aaggccaggc agcttcttgc gcgggaaagc cattcttcat gctctttcat
     2881 agatcttgac cccgtccacg agcgccccgg caagcacgtt ccctatcttt cctcgtgcga
     2941 tttcaagcct ctcttcatcg gcaaccagga tatccaccgg aaccgcggaa ccatgatcca
     3001 aaagtgagcg atatgccgca cgggatacct ctcgttcgtt cgacacgccg tttttgacga
     3061 tcagcagatc ataatcgctg cccgtgactt ccgactcccg gcttcgtgat ccgaacagga
     3121 aaatcctatc cggattcacg gccaaaacta atatgtcgac aatatctcct agagtttcat
     3181 ccgcttccag gattctgctc gaatcatcac cgttcatgcc cgcctccgtc tcgaccagta
     3241 tactcccgaa gccttccgga tcatgtcgag atacgcctcg atgcccgtca gcatatccgg
     3301 gatccgcccg aatatcttaa tgatcacggc agtattccat cactcccccc cctccgctcc
     3361 ggaggcgcgg ccggccagga tgtcggccaa gccccgggag cccgggagac gggcttcctc
     3421 gggcacgccc tcggcgtaga cggtacggac ctccggcccg tccagggccc gggcccggac
     3481 gggatccgcg gtcttcagga aaaccaggcc gtgccgctcg cagacgtact ccaggatgaa
     3541 gtccaggtcg ctcggccccg ccaggaccag ggtacggacg ccctcccgct tggcggccag
     3601 gacgtgctcc tccaggagat cccggtacag ggcggtactc cgggcggtgc gcttgaggta
     3661 ccggtaggtg cgccgcgcga tctcctggac cccttccggc gtcagggcgt agtggaggtt
     3721 ccgggcgttc acccgcttga ccgtcaccca gcccttctcg gtgaagcgct ttaaaaggac
     3781 gttcgtcatg cccagggaca gtcccgccgt ccgggccagg gcccgttggg tgggcgcccg
     3841 ggaggattcg gccttgccgg atccgtagag gttttcgagg accgccagtt cggtgtcggt
     3901 ttgcatgctg tttggttttc cggatgggca ggatggggcc ggcgccccgg gggagcgctt
     3961 ttttccccgt agtcacgacg ccccggccgg gggtcggagg cggccgcaac gcgctccccg
     4021 tcccggaggg gtccttgagg ggcggggcgc gcgaggtgct tttagggctc ctcggccgcg
     4081 gccgcccgag ttgttcaatc cgtgaacaac actatagcgc ggaggttacg gaagatcaac
     4141 ctgggggccg agatattcga cgaatagccg cgaaggaggg cctccaagga cggttcggct
     4201 ttccgaactc ccggtacccc gggcgaccct tcgcggattg acacggacct tagatacgca
     4261 taataggtat aggtatgacc ttccagccgc ccccgaacgt cagcgtcctt ccctataagg
     4321 ggatgattcc ccgcatccac ccctcggtct tcctctgcga cggggtgcgg atcatcgggg
     4381 acgtggagat cggggaaggc tcctcggtct ggttcaacac ggtgatccgg ggggacgtca
     4441 actcgatccg gatcggagcg cgggtgaacg tccaggacct gtgcatgctc cacgtcacca
     4501 agcgcaagtt ttctcttagc atagaggacg aggcttcggt ggggcactcg gtgtctctcc
     4561 acggctgcgt gctcaaatcc ggatgcctga tcggcatcgg ggcgatcgtc ctggacgggg
     4621 cggtcgtcgg ggagggcgcc ctgatcgccg cgggtaccct ggtgcgggag ggggtcgagg
     4681 ttccgccgaa caccctcttc gcgggggtgc cgggcagggt gatccgggag ctctccgggg
     4741 aggaccggtc ccgggtggcg gccacggccc cccactacgt ggagtacgcg gcactctacc
     4801 ggtcgggctc ctaggcggct ccggccgcgg cggccgaacc ggccttccga gggcgcccgg
     4861 gccgaaccgg accgcctccg ttccgctttc ggatatattt tcatcctttc cattgacgcc
     4921 gcaaattcga ccacctacac tttcagaggt tttgtatcca cccccaggtc ccgtgcgtcc
     4981 gggagaagga ggagtcgatc cgcacgcctc agaagaggcg gcccacccca actccttaag
     5041 gaggagcaac gcatgagctc gaaagtcatg aaagttccgc acacctacgt catcctgttc
     5101 gccctgatcg tcgtggccgc catcgggtcc tacatcatac ccgccggcga gttcgaccgg
     5161 gcaaaggacc cgaattccgg ccgcacggtc gtggttcccg ggaccttcca caaggtcgaa
     5221 cagaatcccc tgagtttctt cgacaccttc atctccgtcc agaagggaag catcgacgcg
     5281 gcggacgtgg tcttcttcat cttcttcgtc tatgcgtcct tctacatcgt tctgcagacg
     5341 ggggccctcc attccttcat tggctggttg ctcagagtgc tgaaggggaa agagatcatc
     5401 atcattccca tcttcatgta catcttcgcc ctgggcggct ccgtgttcgg gatgtacgaa
     5461 gagaccttcg gcttcattcc cctcttcgtg ggcctggcca tagccatggg ctacgacgcc
     5521 ctggtaggca tgagcatcgt ctccttggcg gtggccatgg gcttcgccgc ggccttcatg
     5581 aaccccttca ccgtgggcgt ggcgcagaag gtggcggagc ttcccctctt ctcgggcatg
     5641 ggcttccgga tcatcgtctg gttcgttcat gtcaccatgg ccgtggtcta caccctcctg
     5701 tacgcgatca aggtcaagaa ggatccctcc aagtccatcc tcaagggcat ggacatgggc
     5761 agcctggccc tcaaccatga cgagctcgtc cagaagaagc tcaccggccg tgacaagctg
     5821 atcctcctgt gggtggccgt catcgtcggc ctcctggtct ggggcgtcat cgtcaagggc
     5881 tggtacttcg acgagctggc gggcctcttc atcatcatgg gcgtggccgc gggcttcatg
     5941 gccggctggg gacccagcaa gatcgccgtg accttcctgg aaggcgccaa ggacatcgtg
     6001 ttcggcgccc tggtggtcgg tctctcccga ggcatcctga tcgtgatgcg ggaagccaag
     6061 atcatcgaca cggtcatcaa cgccatggcg actcccctgt cctacctgcc ccggtggctg
     6121 gccgcggagg gcatgctgat cgtccagacc ctgatcaact tcttcatccc ctcgggctcc
     6181 ggccaggccg ccacgaccat gccgatcatg gcccccctgt cggatctcct gggcatcacc
     6241 cgccagaccg ccgtcctggc cttccagttc ggcgacggct tctccaacgt cctgtggccc
     6301 accacggccc tgccggtcat ctgctccatc gctaaagtgc cgatcgacaa gtggtggaag
     6361 tacttcgtgc ccttcttcgg ctggctgctc gtggcccaga tgatcttcat ggccatcgcg
     6421 gtggccatca actggcagta gcccggatct tgtagtaaga taattcacga cgggcggcgg
     6481 gggacggtcc ctcgccgcct tttgttttca tgaaggggga acgcatgagc ctggacatcc
     6541 tggtacgggg ggggaccgcc ctggatcccg ccgcgggtac ggagcgcgcc ttgaacgtcg
     6601 gaatcgaggg cggccgcatc gccttcctgg ggccggaagc cccgtcggcg gcccgggtca
     6661 tcgacgccgc gggactcctg gtctctccgg gcttcatcga cacccacatg cacgacgagg
     6721 agctggagga cccggacacg atccagctgg ccctgctccg tcagggggtg accaccgcga
     6781 tagccggcaa ctgcggctcc ggccccctcc tgcgggacat ccgggccgcc cggccggccc
     6841 cgtggctcaa cctgggctac cagaccgggc accgctacct ccgccgggag gtcggcatcg
     6901 acgacgagta tcgccccgcc acggaggcgg agatcgcccg catggaggcc ctccttcgga
     6961 acgagctgga cctgggctcc ctggggctct ccttcggctt ggagtacgcc cccaacacga
     7021 gcccccggga aatcgacgcc ctgtgcgccg tggcggcccg gtaccccgag cgggtggtct
     7081 cgtcccatat ccgctacgac ggccccgaat gcctaaaggc cgtccaggag atgatcgacc
     7141 tggcccggcg ccaccgggta cgggtccaga tctcccacat cggctccatg accgccttcg
     7201 gccactcagc cgaggccctg cggatgaccg accgagcccg gtccgagggc ctggacgtca
     7261 ccatggactg ctacccctac gcggccttct gcacctacat aggctccgcg gtcttcgacc
     7321 ccggcttcga ggagcggtgg aaaaagggcc tggagagcct ggaagccgcc tcgggcaagt
     7381 acaagggcgg ccgcctgacc cccgcctcct acgaggacct gagggccaac gatccccacg
     7441 ccctgatcat cgcccacgtg atgaacgagg cggagatccg gctctgcctg acccacccga
     7501 agtgcgcggt ggcctccgac ggggtcctgc accagggcca gggccatccc cgggcggcgg
     7561 gcaccttccc ccgggccctc cggtggctcc gggaggcggg gctctcctgg cccgaggcgg
     7621 tccgccacgc cacgagcctc ccggcttcca tggccttcct ttcctcgggg gtcctggcga
     7681 tcggagcccc ggcggacctg gtcctcttct ccccggagcg cctcaaggac aaggccacct
     7741 tccaggacca actggccccg ccggagggga tcgagtacgt gatcgtgaac ggaaaggtcg
     7801 ccctggaaaa gggagaagtg acccgccccg cggtcggccg ccttctcacc cgtcccccga
     7861 tgaaataagc gatcgaaagg attccatcca tggactacaa gcagtttctg cccgagctgg
     7921 acgccgtcgt cgaccgcctg gccccccggg cgatctccct gagcgatgca atggccgcgg
     7981 atcccgagat ctccgaacag gaggtccgtt ccagcaaggc ccacgcggat ttcctccggg
     8041 acgcgggctt ccgggtggag caccccttct tcgggatccc cacggcctac aacgccacgg
     8101 tctcctcggg caccggcggc aaggttgccc tcctggcgga gtacgacgcc ctgccggaga
     8161 tcggccacgc ctgcggccac aacgtccacg gggccatgtc ccttttggcc ggggccgccc
     8221 tggctcccct gatggggaag atcgggggcg agctctgggt cacgggcacc cccgcggagg
     8281 agtcgaacgg ggccaagatc ctcatgtccg cgaagggcct gttcgacgac cgggacctgg
     8341 ccctgatgat ccattccgga ggggagaagt cctacgtccg gtatcgctgc ctggccatgg
     8401 attccttcga gttccgcttc aagggcaaga ccgcccacgc cgcagcctcc ccctgggagg
     8461 gacggaacgc cctgaacggg gcccagctct tcttccacgc cctggacatg ctgcgccagc
     8521 acgtccgccc ggacgtccgg atccacggga tctacaccca gggcggggcg gcctgcaaca
     8581 tcgtccccga ggcggccacg gtccgcttct acttccgcgc cgcccggcgg gcctacctgg
     8641 acacggatgt caagaaggtc ctggaggccg ccaagggctg cgcccaggcc acggacacgg
     8701 aggtggagtg gagcaacttc gagctctcct tcgacgagat gcggcccaac gacgccgcgg
     8761 agacgatgat ggaggaggtc ttcaccgagg ccggggcccc cttcggcccc tccccgggcc
     8821 cccagggctc ctcggacgtg gggaacgtgt ccatgcgctg tcccgccctc cagcccaccc
     8881 tgtccatcat cgacaagccc tacacctggc acacccggga gttcgcggcc tcgaccacgg
     8941 ggcccctggc ccacgaggcc ctggtcaagg gtgcccggat cctggcccgg gccagtctcc
     9001 gggccctcct ggatccggac ctgcgcgccc ggatgcgcgc cgacttcgag gccgaaagga
     9061 accgccatga ctagatccga tcgcgcccgg gagctccacg cctcgggctc caactgcgcc
     9121 cagtccgtcc tggtcgcctt cgccggggac ctgggggtgg acccgaagca ggcccaccgc
     9181 ctggccacca ccctgggggc cggcctgggc cgccgccaac tcctctgcgg ggcgatcaac
     9241 gggggagccc tggccctggg agcggccttc ggcaacgagg agggctcgga cctggcgacc
     9301 aaggagctct gctacgagat cgtccgggac tacctgcgcc gggtggagga gatcgccccc
     9361 tccacggaat gccgggtcct cctgggggtg gacatctcca ccgccgaggg tcgggaggag
     9421 tccaagcgcc tgggcctgac cgccaaggtc tgcgatcccc tgatcgggaa gtgcgccgag
     9481 atcgtggagg agatcctggc ggagcggggg agaatcgccc ggtgaagtcc gaggccggcc
     9541 ctccctgggt ccggtaccgc cgcctcatcg acctggaagg tgtcccgccg gccgccccgg
     9601 agtcccgggc ggcccttgcg gacctgctgg ccgcgcccga ggtccgggtc ctcctttccg
     9661 acgtcgcctc ctggcccggc accgtcctgg ccagccaccg cagtccctcc cagaccttcc
     9721 actccctggg cttcctggcg gagctcctgg ccgggtcgtg gctcggggac ggggtcgctc
     9781 cggcgatcgg ggccgcggtc gcccgcatcc gcgagcggac ggggcccgac gggatccccc
     9841 gcctgcccat gacctacccc gagcacttcg gaggcagcgg ggtcgagacc tgggcctggg
     9901 ccctctgcga cgcccccatc ctcctgcgcg cccgggtccg cttcgggggc gcctcggagc
     9961 ccctcgtccg ccggggaatc cggtccttgg tcgccctgtc cgagcccttc ggctggccct
    10021 gccgggtcgc cccggagctg ggttccttcc ggggccccgg caagaagtcc gatccctgcc
    10081 ccttcgccac cctgatcctg ctggagctgt tggcgtccct ccgggaggag gccccggcgg
    10141 atcccgaggc ccgggacctc gcggagggcc ccgaggcgga cgccggcatc gaggccctcc
    10201 tggactcctg ggcgcggagc gcggagcggc acccctacat gttctacatg ggaacggatt
    10261 tccggaagct aaaggccccg gggctctggt acgacatcct gcacgtatcg gacgtcctgt
    10321 ctcgcttccc gaaggcccgg ggggaccccc gattcctgga catgctcggc gtgcttcgct
    10381 cgaaggcgcg gccggacgga accttcgtcc ccgagtccgc ctaccagccc tacaaggcct
    10441 gggatttcgg gcagaagaag gaagcctccc cctggatcgg cctcgcgatc gcgaggatcg
    10501 aggcgcggat gggcggtcgg gactagtccg cggtccgggg attcccggaa tccaccatca
    10561 tgggggtgcc ggatccggag gtcagggccc gctccgccgc cagggcgtcc cgccgcatgg
    10621 acacgacgga cgaggccagc acggccgcga tgatgacggc tcccccgatg atggaattgg
    10681 gacccggggc ctccccggtg gccaggaaga cccagacggg gttggcgatg ggctcgatgg
    10741 cggccaccag gatcccctgg acggccgtga cccgcttgat cccgaaggac aggaaaaccg
    10801 cggccagccc cacctggaag acccccaggg tgacgatggc cgccccggaa gcccaggtca
    10861 ccttcggcgc cgggaggaag caggcccaga ccagggcgat cacggcggtg aagccgtggg
    10921 ccaggaggtt ggactccagg ggggacccgt ccttctgcat ccgcaggaag acgtagtaca
    10981 tcgcgaaggt cacccccgag gccacgcccg ccaggtcccc caggaggctc ccgcccccga
    11041 ggtcacccag gaagaagagg accatccccg ccgccaccgc caggaaggcc gcgaagtgct
    11101 ccagccgggg cttctccttg agcaggaaga tccccaggat cgccgtgtag atgggcgccc
    11161 cgtactggag gaggatggcg ttggccgagg tggtggcctt gttggcgtac acgaacagca
    11221 gcatcgtggc cgcgctggac agggcagccc cgagctgggg gaaggagaag gtgatccggg
    11281 gcttgcggat ccaggccagg aggaagagcc ccgcgatggc cgagcgtgcc ccggcgatgg
    11341 ccatgggatt ccagtcgatg atcttgatga acagccccgc cagactccac aggagggcgc
    11401 agacggccag ggccgccagg ccgagggtct ggtcgcggga agaggtggat gcgctcatgc
    11461 cggggatggt atcccgaaaa tcgcggaaag gctaggcggg cgaccggccc ggtctcgttc
    11521 ctgcaaatcg gccgggaggt gtcatacgat agatccaaca agatgaacag gatgttaggt
    11581 ggatgaacag gatggaagaa tgaaagaaat cacagatctt ttatctctat cttgttgatc
    11641 tttcttaatc ttgtacatct tgttggctct atggttccaa gccttccagt cagtgtaatg
    11701 cctcggatac gcgcgcaaac tcacgccgga aggccttctc caggcgcggg tcgtcggtct
    11761 ccaggacttc caattccagg acctccgccc cctggaaggc cgcgaagcgt gcgagctcgg
    11821 ccgcgacgga ccgggccagg tcggcgggtt tgacgggatt cagcgcggca tcggccgagc
    11881 ctgagggcgc aacggagccc ccgcgggcca ggcgcagcct ccgcaggacc agggtccggc
    11941 gccggcggtc cgccttggcg tccagaagcc cggcgaatcg cgcctccccg ccgtcccccg
    12001 gggcgtacag gatcggcagg gtgaagtatc cgaaggcgcg tttggccgcg gggacgtagc
    12061 actccagggt gtagtcaaag tcccagagcc gggcggtcct ccgccgatcg atcaggaagg
    12121 ggtcgaaggg ggataagacg aaggcccggg ggcgggccgg gaccgcgggg ctcgagtgtc
    12181 cggctccacc gtccgccccg tcgacaccgg ggtctgtcgc cgtttgcccg aggagatccc
    12241 gccgggcata ccagccgggg cccaggccct cgacggccgc ggggaccagg gtccccgcct
    12301 cgaccgcggc gtccagggcg gcgtccatcc cggccaggcc gtccttccgc tggtaggccg
    12361 cctgggcctt cgtgaagatc cccagggacc gggcggcgcg ttccacgtgc cgagcggaaa
    12421 gctcggcctc cgtcggggcg ggtcccgcgt gggccgcggg gatctgccgc tccgcgaggt
    12481 cgtagacctt ctggaagccc cgccgcgtcg cgcaggcgag ctcgcccgag tggaacaggt
    12541 actccagggc caccttggcg ggcttccagt cccaccagcc ccgggggccg ctcaggcttt
    12601 cctggaagtc cttggccgac aggggtcctt ccgcggccac ccggtcccgt acccgggaaa
    12661 ccacctcggg ttccgcgcgg aaccactcgt ggccctccgc ccggatccgc tccatccggg
    12721 gcaggcagta gcgccactcc tccacgggaa ggtaggcggc cgcgtgggcc cagtactcca
    12781 ccaggcggcg ttcgcctccc ccgacaccct ccagggccgg aacggccgaa aagcccgggt
    12841 cggggacacg gctccagagg atgtgctcgt gggcgcggcg gaccaccgag atggtgtcga
    12901 tctgcacgaa gcccagggcc tccaccgccc gggcgacgcc ctccgtcccc gccgggaagg
    12961 cggggggaaa gcccgccccc gcggcaaggg ccaaggcgcg ggcctcccgg aggctcaggg
    13021 gctcgggccg gccggccgcc gcccggctca cggggcggcc ccggcacccg gcgaggtccg
    13081 atcccggccc ttcaagatac ctccaccccc gccgccttcc cgtaggcgcc caggaccacg
    13141 agggcccgca gggtgttcca gcggctcggg ccgccggtct tctccatgtc gaagtgcacc
    13201 gccccgggat gcttccgctg gaccggccag gtcccgtcct tccggcgctt cccgcgcagg
    13261 agggccaggg cgtccgtcat gcgcgggtcg aagggccggc ccgcacgggc gaagtacgac
    13321 agtgcccgga ggacgtcgta cttccaccgg caggggaagg agaggtacgg gaactcgggg
    13381 ttggcgatcc gcccgctcct gagcccgaag tacaggcgcc gggccaggag ccattcctcc
    13441 ccgccccggg aaagccggtc gagctccatc gccggggcga ggcccgcctt tccggcgtcc
    13501 tcgagggctt ccagcacgga gagcgtcgtg gcgaaggagg acgccgcgga ccggacatcg
    13561 aagcagttcc agcccccgtc gggctggcgg tgggccgcaa ggtaggagag gatcctgcgg
    13621 gcccgctcgt cccggatccc cgagtacagg accagggaaa ggaccatggc gctgacgcag
    13681 acgtcggcgg tccggacggt cttggccacg tagatccccc cgtcgggtcc caggaagcgc
    13741 tccagcagga gctccacgcc ctcccggaag gcggggtggt ccccggggaa gcccaggtta
    13801 aggaggtcca tcagggtgta gtgggtgctg acccacttgg gcatataggg gcccccgccc
    13861 cacatccggg ttccgggatc ccgggcttcc aggaagcgcc ggccccagcc ttcgaggggt
    13921 atacgggcgc gcagggaggc cagttcccgc tcgtcccggc cgaggaggtc ccggcggacg
    13981 cggtattgga ccgacacgtc tccggccagg agccaggcca ggaccgcggg gtcgacgccc
    14041 gccgccttct ccccggcggg agcccgggaa gggctcactt caggcccgag gccttctcgg
    14101 ccagggtcac gaactcggcc cggcggtcgg aatccccggc ggcgccccgg gccaggtcca
    14161 ggaccagctt ccagtccgcc cggccccggt gctccgagcc ccggaggatc atgccgtagg
    14221 cggccacggc ggccgcgaag cgcaggtccc cggaggcgtt cgacagggag ccccgggcgt
    14281 ccgcaagggc caactccagc tgggcgctct ggtcctggtc gggcttcttg taccgcaccc
    14341 gcaggatacc cagacccgct ccgggggcgg gggagacggg ctcgatctcg tacagggcgg
    14401 tgacccggtg gcccgccccc atctcgcccg cgtccttctt gtcgtccgtg aaatcctcct
    14461 tggccaggat ccggttctcg tagccgatca ggcgccagga cttcacgacc gcgggatcga
    14521 actcgacctg gagcttcacg tccttggccg cggtcagcag ggtcgccccc atctcctcga
    14581 tcaggacctt ccgggcctcc tccacggagt cgatgtaggc gaagttcccg ttgcccttgt
    14641 ccgccagggc ctccatcttg gcgtccttgt agttgcccgt gccgaagccc aggacggaga
    14701 ggaagacgcc ccggttccgg taggactcga tcagcttcac cagctcggac tcgctggaca
    14761 ggcccacgtt gaagtccccg tcggtggcca ggatcacccg gttgttcccg cccttgcgga
    14821 aattctgcag ggccgtgtcg taggccagga cgatgccctg gccccccgcg gtggagcccc
    14881 cggactccag ggcttccagg gccgcgagga ttttcccttt ctccgtgccc ggggtgggcg
    14941 gcaggacgag gcccgcggat ccggcgtagg ccaccaggga cacccggtcc tggggccgca
    15001 gggtctccgc caggagggag aaggcccgct tcaccagggg cagcttgtcc gggtcggaca
    15061 tggagcccga ggtgtccacc aggaagacca ggttggccgg gggcaggttg gccgcggaga
    15121 cggtccgggt ccgcaggccc agggccagga ggacccggcc ggagctccag ggggaggcgg
    15181 cggactccgc ggaaagggcg atgggctccg cgcccgcggg ctccttccag ccgtagtcga
    15241 agtagttgag gagctcctcg atccggaccg cctcgggggg aggcaggcgc ccctcgttga
    15301 ggaagcggcg gatgttggag tacgaggcgg tatccacgtc cgcggagaag gtggagaacc
    15361 gttcgtcctt ggcgagcttg aagggatttt ccggaagtcc cttgtactcc tcggacgagg
    15421 gggttccggt tccgtccgcg ggcggagggg ccgcccggtc catcatcatg gacttctggg
    15481 ccatcggggc gggaagggct agttcctcct tgagctcccc ttccggcggc cggccgacca
    15541 tgggggacgg cctggagatc gagcaggaag cggccaggac cgcggtaagg gcgaggaccg
    15601 cgagggcggc cgtcggagct gcgaggggac gtacgttccg gccggatcgg gacttcctgg
    15661 acatgggcga acctcccggc gtccgcggag gcggagccgc ctctactata tcccggagcc
    15721 cgggggattt cgattcccgg ccccggggcc ctcatttttc cttcccgcgg ttgcatcttt
    15781 cccggtgccg tgcaaagatc gaagctcgcg ccgagaagcc ctcggccggg aggtcccgtg
    15841 ttccagcccg tccaggtcat cttcgccccc acgtcccggt gcaacctcgc gtgctcccat
    15901 tgccgggtcc ggcgcggccc cgaggagctc tccgccccgg aggcctcggc cttcctggag
    15961 cgctgcgccc ggggcggtat cgagcgcgtc gggttcagcg ggggcgaacc cttcctccgg
    16021 cccgacttcc tggccgaggt gtcccggacc gcggtcgcct cgggcctcta cttcgaccgg
    16081 ctgatgacca acggcgactg gttcgcggac ccggccgacc tcgaggagtc cctctcggcc
    16141 ctgtacgagg cggggttcga cgggaccttc ggggtgagcg cggacgcctt ccacgaccag
    16201 gaccccgggc gcctggcggc cttcctgtcc gcggtcttcc gcatctgggg ccggaaggac
    16261 tgcgccgaga tcctctcggt ccgggccccg gacgagggtc cctggctcga gggcctgcgc
    16321 gtcctggcgg agcgcctggg cggccgcctg gtcccccgga ccggcgaacc ccgggggatc
    16381 gcggacgcgg ctccccgccg ggaggaggcc gcggccgggg aggtcctgga cattccgatc
    16441 ctgcggttcc cccgctccgc cgccgggacc gagaccggct ggggcgaaga ccggtggttc
    16501 cgggaggact tctgcgaggg tccgggcaac gtcttctacg tccaccccga cggctccgtg
    16561 gccgtctgct gcggtttcgc caacgagaat ccctccctga tcgtcgggaa catccgggac
    16621 gacgactacg acagcctcat ggaatccgcc cgggcgaatc cccacgtccg cctggtctac
    16681 gagaagggcc tggacgcgcg ccgccgggag ctggaagcct cgggggtgaa gttcccgggc
    16741 atgacagacg acatctgctt cttctgcgac cacctgtata ggaacaagct gatcggggat
    16801 tgacaggatc cgtcgtatag attacttata ttataatcat tcccgatcat ccggcggaaa
    16861 gcccacggtt cgcggcgcga ggggcccccg cggaggaacc atgtcccgca cccgagaacc
    16921 taagggcaag atcgtacgcg ctctcgggct gaacgtcttc ggaaacccga agttcgaccg
    16981 tctcctggat cgcaagcccc accccccggg aaagcctccc cgctactccc cccgcaagac
    17041 ctcggaatac gcggaccagc tccgggagac ccggaagtac cggaccgcct acggcctggg
    17101 acgtcgccag ctcaagaacc tctacgagaa ggcccggcgg gcgggcggct ccaccagcgc
    17161 cgccctgctg gtcttcctgg aggcccggct ctccaacgtc gtctaccgcc tgggctgggc
    17221 ggtctcccgg gcccaggccc gccagctcgt cagccacgga cacgtcgcgg tcaacggccg
    17281 gaccctggac atcccgagcg cggccctgcg ccccggggac gccctggagc tccgggcccc
    17341 ggagtccctg gcccgcctcg tccgcgaacg gacggcctcg gaaggggccg cgcgcctgcc
    17401 ctcatggctg gcccgggaag atggaggccg ggcggcccgg gtggcatccg cgccggtacc
    17461 ggaggaagcc tcggtcccgg gccgcatagg acgggtggtc gagttcttcg cccgatgagg
    17521 ccttgcagag cctcccggga atcgatagta ttttaacgga atttcgcgcc cggcccggtt
    17581 tttcgcggcg gccgcgatat agtgttccac gtgcgccccg tacgggggca cgtacctgct
    17641 cgcctgccca aggggagtgc catgaaacgt atcgtgtttt tcgtgatcgc cgcggcgatg
    17701 tgcgcgcggc tgcccggggt ctacgcccag gacctttccg aaccggacga acaatccgta
    17761 ctgcagaagg cccgcgacaa gtacggccga accgcggagc cttcggatcc ttccagcgcg
    17821 accgacccct cggacgcgga ggattccggc cgaccgttct cccccttcgt cttctccttc
    17881 gtccccggca tccagttccc gcccttcggc ctctacgaca cctccgccgc cttcggctgg
    17941 atcgggtccg gggtcgggaa cgtcgaggga atccaggcct ccggggtctt caacctggcc
    18001 cgggacgtgg aaggcttcca gggatccggg gtcttcaaca tcgcccggga cgtggagggc
    18061 ttccagggat ccggggtctt caatatcgcc cgggacgtgg agggcttcca gggatccgga
    18121 gtcttcaaca tcgcccgtaa agtggaaggc ttccaggctt ccggggtctt caacatcgcg
    18181 gacaccgtgg acggcggcat ggtcgccggg gtcttcaacg ccgcggaacg ggtctccggt
    18241 cccatgatcg gactcgtgaa cgtggcggac cggctggacg gggtggccct gggcctggtc
    18301 aacatcatcg gcaacggggt caacgagatc ggcctccagt acatacccgc cgaggacatg
    18361 acctacctcg cctaccggac gggcaccgcc tccctgtact cggtgtacta cggaggcgtg
    18421 ggatccgagg actggttcgt ggactcggac tccctggtcg tgggactggg atggggccac
    18481 cggatccgca tcggcgacgg cggcctggac ttcgaggtct gcgcggaaca ggagctctac
    18541 gcggaccgca gggccgcgat ggaaacggcg gcggacgccg acgacgagga cgccttcctg
    18601 gccctcatcc ggccctatcc ctccgtacgg gccgccctga acatcccggt cctcggtttc
    18661 aaggccgtcc tggggatcga gacggacttc gacgtcccgg cctggggatc ctatgtcccc
    18721 gaccgcctcc gggcatcctc gttccggacg ggcgggtgga gcggggacgc ctggggcctg
    18781 gagttcaccg cctggcccaa ggtcttcctg ggcctgagct tctgagaccg aggggcgtgc
    18841 cgggaacggc ccggcgcgac cgcccccggc ccgctcgatt tcccgctacc cgcgagatat
    18901 actgaaccgt gaacccgggg tatccgaggc cggaatcttc cggcctcggg cagcccgatt
    18961 ggactaggga ggagcgaggg atgaaacgcc tgatcgtcat agccgcgtgt ctgctcacag
    19021 tcgtgtcggc cgccgccgcc cagactctct tcggggagat cgagtacgta gagggcgacg
    19081 tgaccctgag ccggggcggc aagagcctgg gggacctgaa catcggcgac gaggtccagg
    19141 tggacgattt catcaagacc ggccccgacg gcgcggcggt catcgccctg aaccggtcca
    19201 cggggatgcg cgggtccctt tccgtcaagc cccgatcggc cgtctacgtc cggatgtccc
    19261 aggactcttc gggctccaag acgaccctgg agctggtggc cgggcagatc ggatccaagg
    19321 tggccaagct ctccggctcc cccaccctcc aggtgcggac ggacaccgcg gtcatgggag
    19381 tccggggcac cgagttcggg gtatccacct ccgtgtccgg caacatcgtg atcttctgca
    19441 ccgaaggggc cgtggagacc tcggacgggg agtccaccgt gcaggtgccc gccggcaagg
    19501 ccgtggaaaa gcgccccgcg gagcggctcc gcctgcttcc cgtcgcggta tccaatccgg
    19561 acgagttcgc caagcgctgg atcgcggacg agatcgaggc cttccgggcc aatcccgccc
    19621 gggtcgtagc gttctacgag aagcgctacg cggacctgag cgtccgcttc acggcggccc
    19681 tggacgattt gcagcggaaa gccgccctgc ggaagtggat cgaggaggac aaggcgggca
    19741 cccgggtcaa tccccgatcc gcggacaccc tccgagaaaa gaaggaactg gtccaaagct
    19801 tcctggagct tcggaagatc ctcttcctct tcgagcgcat ctactaccgc ctggacgagg
    19861 cggtggacct ggtttccggt acccccgggg agcgggcgga gctccggccc ggcctgaccg
    19921 cgggggcctt cctgcggcag ttccgggacg agcgggatag gctggccaag atgatggcct
    19981 tcttccgcta cgccgaacgg ctctacgccc tgcgcaacga gggcggagcg ggcttcccgg
    20041 gcctgggcgg ggacgacgac gacttcttcg gcacttccga tttttgagag gaaagcccgg
    20101 aaccatagat ccgaacgctg aaactgtgag gtaaccttga cgaccgtgat ctccctgggc
    20161 ggctccatcg tcgccccgga caagcccgat gtccccttcc tgcgggattt cctggccctg
    20221 tccacggcct ggctcgagga ggatcccgag cgcaggctga tcctggtggt ggggggcggg
    20281 ggacccgccc gggaatggca gaaggcctac cgggagacgg cccgccgcgc cgatccggac
    20341 gcccaggact gggtggggat catggcgacc cgcctgaacg cccagctcgt gaaggccctc
    20401 ttcggggagc tctgtccccg ggaggtggtg atcgacccga ccgcggtggg gatcttcccc
    20461 ggacgggtcc tggtcgcggc ggggtggaag ccgggcttct ccacggacta cgactccgtg
    20521 ctcctggcgg agctcttcgg ggccggcacg atcgtgaacc tatccaacat cgccaaggtc
    20581 tatacggcgg accccaggct ggaccccgcc gcccggccca tcgactccat cggctgggcg
    20641 gagttccgcg ccctggtggg cgacgagtgg gtgccgggca agaacgtccc cttcgatccc
    20701 gtggccagcc gcagggccca ggagctgggc ctccgggtgg tctgcgccgc gggccgcgac
    20761 ctgcccaacc tccgggccat cctggagggc ggacccttca cgggtacggt gatcgggggc
    20821 tgatctcccc gatgtttccg gtccggcctt atcggtcctc gatctcgatg taaaggcttc
    20881 cggcgctccc gtccgaggct tcggcttcga atctccagag tccgggcgcg tactcgaagg
    20941 ttttccgctc actcacggag gccgccggga cgatgtccag ggttccccgg acggcggagt
    21001 ccgcgtatac cctcacggtc acggagccgg attccaggct gccggtgacc gccagggtca
    21061 cgtcccggag cggggcgatc gtgtcgctca cgacgggatt cgtgcccggc aagtcccaga
    21121 tgcgtccggt gtggctgtag ctgagcccgg cgcaggacgc gagggtcagg gcgagggcga
    21181 aaaggactag ggggcggaaa cctccgccgg aattcgagtt catgacatct cccggatgac
    21241 ccaggatacc atatcgataa aaaagggtct agacggatcg gggcccgtcg ggcttcattc
    21301 cggggcgttc cggaccggcc tgtcgaggat caggccgctt ttacggaaca ccccggcgtg
    21361 gaaggcgtcg atgatgcggg catggatcgg accgtagagc gacctgtccc ggaaggggcg
    21421 gccgctgcgg taccaggtgg aatgcacgat caccgtggag gggcttccgt cggggccggc
    21481 ccgttccagg aaccggatcc gctggacccc gttggacagg ttccgcttca ggtagacgaa
    21541 ctcgatcagg cgctccggct cgtcgatccg ggtgatccgg aaggacgccg ggatacggaa
    21601 gggtccgagg atccggacgt ccaggacgag gacctgcccc ggcgagaagg ggggagcggt
    21661 ctcgccggcg gcgtagagcc gggacccgga gggatcccag actacccccg ggcgggtacg
    21721 ggaagtcttc cacgccgccg agggttccag ggccgtgtag gccttccaga cgaggtccgg
    21781 cggggccgga gacgagtagc tcctgtcgtg aaagaagtac ccggccgggg attccaggtc
    21841 gctcgactcg tagccggcca ggttccagcc gttcctgtcc aggaagcgct tcaccgaggg
    21901 cgggagtccc cgcccggccg gggcggtcgc gcatcctgca aggaccgcgg cggccagaag
    21961 ggcaaacccc ctccggcgcg ccccggtccg gaggcggccg aacaggcacg cgtcgggttc
    22021 gccgccccgc cccggatcag ggccgggcat cgagggagga tcgtatccgg gcggcgtact
    22081 cccggtaccg ccggacgtcc tcgggccgcc ccagctccgc ggccaagacc gccagggctt
    22141 ccgcgcagcg cagggcggct tccgcggagt ccagggtaac gaagacgtcg aaggcccggc
    22201 ggtagtagtg gaaggcgtcc tccctcgatc ccaggccccg ggacaggacg gccagggctt
    22261 ccaggtccgc cgcgatcccg gcggagttct ccgccttccg gtccgcctcc agggcctgcc
    22321 ggagcaggtc cagggcggcc tcggagttcc ccttcttgtg tgcgatgttt cccagcaggt
    22381 acaggttggc cccgtactcg gccagacgct tgagccgctg gttgatcccc gccgcccgcc
    22441 tgaggtcgga ttccgcggcc gcccactcgc cctgatcccg atacaccacg gcacgatcgt
    22501 ggaggaggac cgccagggtc cgctcgtccc gggcggccag gggcagggct tcctggaagg
    22561 cctccatcgc cgcggctccg tctcctcggg catagagcgc ctcacccagg ttggccaggg
    22621 ccagggcccg gaaggacggg ttcttcaggg gaccggcgta atccagggct tcccggaagg
    22681 tcgcctccgc gtcgtccaag cggcccgcgg ccaggtagac ccggccgatg gagttcatgg
    22741 agatggccac gccctcggtg ttgtccaccg cgcggttttc ccggagggac tcctcgtaga
    22801 agcgctgggc cgaggcgtag tcccgcttgg ccaggaaacc gtccccgagc ttggagtact
    22861 cggccgcctg gtttttccgg gccacgacct ccgcgacggg cttgggcgcc gtggaacagg
    22921 ccgagaggac cgccgcggcc agggttaccg gaaccaggaa ggaccgggcg gccggtcggc
    22981 cccggcgggg ggcgcggccc gggaaggccg acaggtttgc gctcattgga aatccccctc
    23041 ccgtatgctg cggtagagcg actgctgctc ctgccgctcg ggaattccct tgcgcagcag
    23101 cgggttgttg gccaggccct ccagaacatc ctgggtcttc tgcagggtgg tcttgccctc
    23161 ctccaggagg gaggcgatct tgggcatctc ggagctcagg gaccccatga tctcccgaag
    23221 gctccggatg ctgcgctcca cgtcccccat catgcccagg accttgttat agagggcgtt
    23281 gttgtcgtcc aggaaggtct tcagggagcc cttgggatcc aggagcttgg gcaccaggcc
    23341 cgtgggatcc tccatctcct tggcggcctt ggccaggctc gcggagacct cgttgacgga
    23401 ggccaggatg ccctggagct ccgtgagcag ggcgtccgtc cggccttcca cgtcctcgat
    23461 caggttcgag gcccccgcga ccgcccgggg gagttccgag gccgccttgg acacgtcgcc
    23521 gacgatggag cccaggggtc cggtgctctg gcccgccagg gcacggttca cctccgtcag
    23581 ggtccggttc acggtgacca gggtgcggct ggcgttctcc agcaggggat tgaggcccgc
    23641 gatgaggcgg gagacggtgt catcccgggg agggatctcc gcgagctcct gctcgatgac
    23701 ggcctggcct tccgcggtat ccgcggcggg gatgtaggaa ccctcggcca ggacctcctc
    23761 gccctttccc ggatggaaga ggagttggct ccccaggccg ataggggaca cggaaagctc
    23821 caggatcgag tgctggcgga ccttgggata gtaggtgtcg tagatgtcga agaccacgtc
    23881 cacctggttc tcctcggtca gggtgatctt cttgatcttt ccgatctgga aaccccgcat
    23941 gaggatgggc gtgcccgggg gaatgcccgt gccggaggtg aagcgggact tgaactcgta
    24001 gttcttggcg aaccagcgct ggttggcccc caggaggatc acgatggcgc agaggagccc
    24061 cacggctacg aggacgaaga gccctacgat gcggtctgcg tgcttgatgc ggaacttcat
    24121 gaacctctct cctcgtccgc cccgttgtcg gcggacctct cgccttcgga aggttcccgt
    24181 gccggacgga ccacggtgac cggcgactcc ggggcgttcc gggcgcgggc gccccgggcc
    24241 cggagccggg cgaccagtcc cgcggtctcg gggacctcgc tggccacgac ctcgtcgtag
    24301 agccccgcgg cggcgatgcg accgtcccgg atgatcacga tccggtccgc tacccggtgc
    24361 acccagccca tgtcgttggt caccaggagg acggacttcc ccgcgtcccg cagggaatcc
    24421 agcacctcct ggaccctctc cttggcatcc tcgtccaggc ccgaggcggg ctcgtccagg
    24481 aagacgagct cgggatccag gaccagggcg cgggcgatgg agaccagctt ctgctccccg
    24541 gcggagagct ccgcgggccg cagagccagg ccctcgtcgt atcccacccg ggcgagcacc
    24601 tcccggatcc gctcggcgat ctcgctctcc cccatccagt gccggtggac ccgcaggggc
    24661 agggcgatgt tgttgaggat ggtctggttc gcccagaggg cagagtcctg gaacacgaag
    24721 gaggtgcggc tccggaagta ccgctcctcg tcctcggaga aggaagccag ggacgcgccc
    24781 cggaagagga ccgatccgga atcggggacc acgagccccg cggcgg
//
LOCUS       DAOTEI010000034        23374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1441_length_23374_cov_5.685964, whole genome shotgun sequence.
ACCESSION   DAOTEI010000034 DAOTEI010000000
VERSION     DAOTEI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 23374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23374
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1441_length_23374_cov_5.685964"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="P5313_05095"
     CDS             <1..1383
                     /locus_tag="P5313_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRY79773.1"
                     /translation="IAQKVKIIVEHYREHVAWRLGGQAKAMVVTNSREEVIRYKLAID
                     AYIRENRYPIGTLVVFSGDIKVKDLADLPFNESNMNPGLRRRDIKDAFDTGEFQILLV
                     ANKYQTGFDQPKLVAMYVDKKLSGVATVQTLSRLNRTYPGKDWTAVLDFVNNPDSILE
                     DFQLFYRSASLPQGTDPNLLYKLETKLDAAGIYLESEVEAFANWYWDPKRSKNNHFQL
                     QKYLQPPVQRLKERYVAAIEGKDNETKNAIEIFVKDVGSFCKLFEFITQIFDYGDDIG
                     LEMRYSYFKELLPLMLGILRDGRDSSDIDLSGVRLSHYGIRPKDAGRLQLDPAGVSEL
                     PPAYGDIGSGTSRDPETIKLLELIEQLNLIFEGDLSDSDLVNYAFAVRDKLLENELLR
                     TQARNNTRKQFDESDDLHEAILDAVDEQAKVNSDLARQVMSDARKRDEFMKIIRELAY
                     WGFLGEEKAG"
     gene            complement(1426..2223)
                     /locus_tag="P5313_05100"
     CDS             complement(1426..2223)
                     /locus_tag="P5313_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY79774.1"
                     /translation="MNKYLDLPGHGRMYAESEGTGPAIVFVHADFLDGRMWDGVRARL
                     AARHRTVSYDKLGFGRSDPAAVPVVRRKELTAVVDALGLETFHLVGCSNGGQQALDFT
                     LEHPDRVLSLTLVNASPSGWQPQGDMPPLLMEMFAAVQAGDTAAASELQLRIWFDGPD
                     RDKTRFSPEIREARRQASVMNRIFVERGTFFLSDPQAPESPALSRLAEVKAPALVVDG
                     LWDWSDNRRASRFLAEGIPGARRVEVDGGHAAPLEDPAGFAALWEKI"
     gene            2309..3265
                     /locus_tag="P5313_05105"
     CDS             2309..3265
                     /locus_tag="P5313_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRY79775.1"
                     /translation="MRADRLISLILALQDRGKTTAAHLAAALGVSRRTILRDIDALSL
                     SGVPVLAEGGLGGGIWLDPAYRTSLTGMKEAELRTLLAAGDARLLDDLGWGQTFRETQ
                     AKLRASVPRRFEPGVEHMRQRLIIDSRWWWHEAGEPNLEVLQAAVWGDRVIEGEYQTW
                     EGGAKAVRLEAYGLAAKAGLWYFVGRRAGEWRVYRVSRFEVVRASGAFARDPKFDLPA
                     WWSAHAAEFAGELAGFRFSLGLPEDRVRYLRGIAPGRVDVVGPWERPGWVRAGVGLDS
                     SLYAELVVLGLGRRCVILDNPELADAVVRRCREGLAAHEGLT"
     gene            complement(3335..5524)
                     /locus_tag="P5313_05110"
     CDS             complement(3335..5524)
                     /locus_tag="P5313_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79776.1"
                     /translation="MIGYRRAEGRPRARSTIAAVLALGTIFPVFPQESMAELNERYSF
                     PVSVGAMYRDLQAFGGFPSGFSFREIGAELRVPLTNLSTVQPFVRAGTLSVLQAGSAG
                     GSQFDHSQMWGALGLGYSTRFSRNFEIGIDAGGGIFQTAFPNLSPGTTVGELGYLAEA
                     GARIGLVPSYNLSVDLRPAIRYSRSLGLLDDFDGLYFGLGFAVSWRFGQDPDAAAAAV
                     RALRLETQKLAPAFAAMQSYYSTRPFAQVALVNGENTELRDIEVSFFQPGYMDAPTPS
                     ATLNSLAPRGRTEIGLLAAFNQEVFRTEGTVPLAGEVIVRYRYRGRPVEQRLPVSYEL
                     LDRTAITWDDDRKVGAFITPADSALRNYGSFVATSLRAETLPQLNQAMQAAAQVYSSL
                     GSFGLTYQADPSSPFVRAQAGAISVDSVSLARATLARGTGDCDDLTVLFASILESMGH
                     ETGFITVPGHIYLAVNTKIPARDFREIHPDRSMTIVQGDRLWIPVEITLVGKQPFADA
                     WRRGAELWKLYDTNPDKRGFYTTAAAQTVYRPVGLRETDLGLQYGSRSDLTAAFRPEL
                     IRLGDFALTELAAQTAKGNDVREWNSLGIAYAKFGRTKEAESAFTRALRIDASFTSAQ
                     VNLGNLAYLQKDYRRALASWQGAVRALDTQGKGRSTSAQMILVNMSLAYNALSDFSQA
                     RTIFERAAEIDPARVRDFAYLASVGAGSGSTGRAAEQSSSVLFLREE"
     gene            complement(5539..8397)
                     /locus_tag="P5313_05115"
     CDS             complement(5539..8397)
                     /locus_tag="P5313_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79777.1"
                     /translation="MSRASRPFTLAAAVLVVILTGCPNPITDDIVLSAKDAESPSILV
                     TTPSEYSSYSRVIRIEGVVLDGAGGGRSGRVSSLSYEILSYTAEKSATVSPSGAFVIE
                     EPNDLRTNITVLLKAVDWNGNAAEYRLPLVYPGNDIPSFQSEEDSRSVTLTWDPVPGA
                     TRYRLYVEPSSKTPDPGISTYFDDVTSPHTLNQLANGSVYSLLLAADGPGGRVNYSQV
                     VRSVPLSRMHLLPRATERYDGIALSWRTFESIPSYTVLRSASPAGPWEDVSGPVVGSG
                     YFDASASGGRRYYYAVKPTSRSTVVSEYIEAASDSVSSRQDAPVAVFDGVENSQSSAY
                     KDGYLYIADFYGGLRVLDVRNPGLPTQAGFLALTQVQSVVLDEDEPYAYLGGYRWTGT
                     AWAIALFIVDIGNPSMPSIAGYADLDPSGFVLQPESVAVLGNLAFVAGFNSGLYVVDV
                     TNKSAPAVRIQARDAALLGQAYDVTVQNRSGTRLVAVAGNSATAVYTVTGTDAVPTVT
                     RNSSSISGAGSVAFTASGTVLYAAQGSFVRAWQTSNPASPSALSAVEPGGSSVLVGEL
                     TVSGNRVFAAMNIYGYSVVDVSNPSSLRTAFTRNVPGSTEHVAVGDGYSFVSTGRDYP
                     FRIYGANDPSAAVLVNTQTGLRSAARLAAFGGRLYVSEYSADWTSSVFSLEAPAVPAR
                     VATDAGGYTPYDFSFAGERSYIAAERSGVMQWSLQTPGAPTHAPQVLPPWYVSTQGGN
                     AWSVALFGNYALMGTSNSWLNVVDLSRASQGFLTVVAAVTTQPSPGANREARRIALAG
                     SYAFVANEAAGVRAVSVQDPEFPSALAGYGSPWSTGETAAVAVYGDYVLAADTLGGLL
                     VYEASNPRAWSAGGTAHAWRQPSAVPAFDLAVRGSYAYVAYGAAGLGIWEISNPLAPL
                     QVGTVQAAGFEPRSLTIYGDYLYAADGGSKLYTVSLRP"
     gene            8582..9517
                     /locus_tag="P5313_05120"
     CDS             8582..9517
                     /locus_tag="P5313_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY79778.1"
                     /translation="MNRKKYPILLSVLLAALVVLSACSPKPPVTPVTVATMVDSEGAI
                     LGKGMILLMEAKGIPVTDKVGFGTPDILRKALVAGEVDLVVDYTGSGQYYHEDLDTTV
                     FNDPVKGYELIRDTDRKRHDLVWLAPAKANNTEALAVLRSFGQENGLRSMKDLAAYVN
                     AGKPVKLICAVGFAENPLGLPGFEKAYGFKLRKDQLILLESGNTAQMLKALVDGTDGV
                     NVSLVYGTDGALDKMNVLVLEDPANVPPVYLPTPILRGAVLEKHPALKDLFTAFFESL
                     DLVTLQRLNARVAFDGEDPKEVARSYLKERGLLKN"
     gene            9543..10709
                     /locus_tag="P5313_05125"
     CDS             9543..10709
                     /locus_tag="P5313_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY79779.1"
                     /translation="MTVDWIALAGAGAGLVSAAALPFLSLRPNRILAGVPQYAMEVLG
                     AGSVLIFTAAAAAAVLAALRRRHSGLDAAGTLVSGAGLASLLPLAGAAADRLAGDSAV
                     ARVSAGPGLWLAAAGLGAALFSFSRRLPSRSALRLAAALAIPAAAAAAGLAGLLDDLS
                     LVRELLSRRDTFTAELVRHLALAGGSAALGAAVGIPLGLAMHRRPRWRGLISVAVSGA
                     QVIPTLSLLGLLIVPMAALGAAVPYLGRIGVRGVGWAPAFVVLFLYALLPIAGNTFAA
                     LGAVAPEALDAARGMGMSRRQAFLRVQAPLALPVILAGVRTAFTANMGNAILAALVGG
                     GGLGTLVFLGLAQAAPDLILLGALSTSTLALAADRALALAFRLAVSRGLRSEAP"
     gene            10706..11653
                     /locus_tag="P5313_05130"
     CDS             10706..11653
                     /locus_tag="P5313_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79780.1"
                     /translation="MIRFRNIVKSYGSARAVDGADLEIAPGELCVLIGPSGCGKSTLL
                     RTANRMVEPDSGEVFVDGRPVREHPPEELRRGIGYVIQSVGLFPHMTVGDNVAVVPRL
                     LGIDKGEIAGRVDRMLELVGLSPEWSSRWPRELSGGEAQRVGVARALAADPPILLMDE
                     PFGSVDPLTRVRLQREFLAIQRRLHKTVLFVTHDVEEAIRLADRIAVMRSGRILRHAT
                     PEEFVATDAEDFVGEFLGLEYGVEILSRRRVREVLRPAAEIGSDPGAPSIRAEASLKE
                     ALGLMAARRVECLSVTDGEGRRAGSLRMEDVLGTWKACP"
     gene            11650..12450
                     /locus_tag="P5313_05135"
     CDS             11650..12450
                     /locus_tag="P5313_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79781.1"
                     /translation="MNDASGNPAAAQADSRRLRAPGWVFPALLLAVLILSVLRFDLIE
                     SLLGIRARGLLVSRIPLWELALQHLGLVAASSAAAVPIALGLGIAAVRFSESGLRALL
                     VAAGSAAQTFPSAAVLALSVPLAGFGFAPTFLALSLYGMLPVLHNTIAGLEGVPEAAV
                     DAARGMGMRPRQVLIRVQLPLALPVILAGVRTSVVINTAAATLGAAMGAGGLGLPIIS
                     GIRSFDPALILRGAVPSALLALFADASLRRLEDRARRAAGGKPDGRGA"
     gene            complement(12494..14155)
                     /locus_tag="P5313_05140"
     CDS             complement(12494..14155)
                     /locus_tag="P5313_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY79782.1"
                     /translation="MPHSPESIRRLLLDLVAIPSVSPDPRTEDAAVRYLRDRLTMLSW
                     FRDHPEDLALLPCEGDPLGRSFLFALLRPRPGLRDTVILTGHIDVVGVKVCGPLAPYA
                     FDPEGYGTRIGGMPLSPSARRDLESGEWLFGRGVADMKCGAAVATALLEDFAEEHAAL
                     ETNLALLFVPDEEDSSRGMLGAVRHLARLQDGGLRFLACINTEPSIGTGDRLSDLAAV
                     CTGSAGKINPFFFCVGREAHLGEYYEGLSAALLTSQINLLIEGDPARADRGGREALTP
                     WACMKHRDFRHEYSCSVPERSASYYSYFTAETLPGRLLEEMRRIAGTALRLSIARTRK
                     NARRFSRMGKPRPAALDLEPRVLTYAELEGRARAAAGPDWEAAVEAAGNALPPGSDAR
                     DRALARVDACVTLAGEKGPLVVIGYLMPWYPHRGNDGRSVGDRAMLALARDLRSFAAS
                     LGSPLSIRPYYEGISDLSYLAAPCGKADLAAFTENVPGYGPLYELPLEALDRLEVPVL
                     NLGPVGKDAHKLSERIHEGFAFKTYPKLLRRAVLGAGKTGASGML"
     gene            14457..15107
                     /locus_tag="P5313_05145"
     CDS             14457..15107
                     /locus_tag="P5313_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79783.1"
                     /translation="MNRVWMIAVCIAIGAGGLSAQPAWQKHMGEEPVSEFSLSGPYAK
                     AELGAIRTELGDREFGELTLARLEPYWTRMNLALSKDQYLERVSKMSMILPGTGQFEN
                     GDTSKGVGFLSLHIAVVTGTLAGFYFLLPADLRFDRLDYFKTSFKDINDTWEAHSLND
                     YLPSIGMMLAGTLVDLGVRFWASGDAYAGARAAVDSGRAELKPVVGPGFLGVGMSY"
     gene            15481..15978
                     /locus_tag="P5313_05150"
     CDS             15481..15978
                     /locus_tag="P5313_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRY79784.1"
                     /translation="MRTVKQRILSFLGLWGVWLLLTAPWNSEEAAAGAVVALLVAALP
                     FSAASPLGDLRLGPRALATLVAYFFVFAKALVLSNLDVAFRVLHPRLPIAPGIVKVKT
                     RLKTPLGRLLLANSITLTPGTITVEMRGEDIYVHWIKVADSDPEGATAAIVGGFEKYL
                     EVICG"
     gene            15971..16231
                     /locus_tag="P5313_05155"
     CDS             15971..16231
                     /locus_tag="P5313_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRY79785.1"
                     /translation="MADTVFAAASGLAVLGLLLSAVRFARGPWAIDRTVALDVMTIIA
                     TSLIVAFAVFSGRRIYVDVGMVYGLVGFAGVVAVARYLEGGL"
     gene            16233..16577
                     /gene="mnhG"
                     /locus_tag="P5313_05160"
     CDS             16233..16577
                     /gene="mnhG"
                     /locus_tag="P5313_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRY79786.1"
                     /translation="MNETIGAFVSLAGSLFFLLSAIGLLRMPDAFNRMQAGTKATTLG
                     SILFLLGVGIMKPDWLGRMIILIVFIVLTNPVSSNALARAAHAHRKRIGARLEPDALA
                     AADEGKEAGHAG"
     gene            16567..16857
                     /locus_tag="P5313_05165"
     CDS             16567..16857
                     /locus_tag="P5313_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRY79787.1"
                     /translation="MLVDAAILVSGFVMIAAGIGAVLARSLLVSILLSGGVSLVASLV
                     FLLMGAPDVAMTEAAIGSGLTTVVYLYAMARARQPDEEDADPLGDAVKGGRR"
     gene            16854..17555
                     /locus_tag="P5313_05170"
     CDS             16854..17555
                     /locus_tag="P5313_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRY79788.1"
                     /translation="MMRRVLVFLAVAGLAAALLPLALGTGVPEAPGALATAYLEDAPG
                     DFGAANVVTAIVVSYRGFDTLGEVAVLFAATAGVGALLTRKRLGIGDAEPADKPAATE
                     ILVTATGLLTGLLVMFGVYIFLHGHLTPGGGFQGGVVIATALLLGLLARPESKLSHGI
                     LSAVESLSGAAYVGMGLAGAFLAWGFLDPRVLPLGRIGSLVSAGSIPVIYSLIGLKVG
                     AELSGILDALKGDRE"
     gene            17552..17932
                     /locus_tag="P5313_05175"
     CDS             17552..17932
                     /locus_tag="P5313_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRY79789.1"
                     /translation="MSILEFVSRINPVAASGAVLVLVGVWGVLARPNLFKIAIATSVL
                     ETGVNLVILSTGYIRGRTAPILDSAADASTAAARMTDPVPQALVLTAIVIGLAVTALQ
                     LSYAVRLRAAGGGSSIESYGDEKW"
     gene            17926..21015
                     /locus_tag="P5313_05180"
     CDS             17926..21015
                     /locus_tag="P5313_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY79790.1"
                     /translation="MVNPLWLFALSLGAAFLLPLVDRLGRRIALALVHGVTAAWLAAG
                     IFWLSILLSGGEAMDFGTAGFMAPLAIVLRVGLAEAAGVTAVALTGLLAASSLTRSLR
                     DAPLGGSMALLMVLMGLSGIVLTRDLFNLFVFLEISAIGTYGFLGFDDRKPALRAAFK
                     YMIAGGLASSLYLIGVIYVYRLADTLNLDIAASGTALSGPAGTAAVFFLLAALLVELK
                     PFPANGWAVDAYETAHPGVSAILSGASATALLLALWKILPLLGPAHLSLVAASGLATF
                     AAGNLVGARQDRPLRMLGYSSVAQVGLVAAVAALARLSGLGDGGSDFAAMVAGGLLLN
                     HLLAKTGLFLLAGAVEDDRARGPLSPLALAAAGILSAALVGLPPFPGFWAKWRLVRAL
                     ADSGRWWILALVLGGSLLEAFYVFRWLGARTKGEAGEAPVRLGIRGAGPVLAALLLVA
                     AGTLGGIRGPVPDLLLWAPWAAAAVLFLLDGIPGRLKGILSAAAAAAFGAAVWGRLDG
                     LGQFFAVMLLGGGALFSIAALRKGGARRGLYPLLSAATLALGAILAARTPLEFFFAWE
                     TMTAASWLLVLRGRDGRKPALSYIVFGLGGAFLLMAGLAAGGWDGGTAAAGWPGLLVL
                     AGLLVKAGALGVHVWLPGSYAESDDESSGFLSAALSKAPVFALFALALSLGAALPGGP
                     WTSRVLGWLGLATALGGALYAVYQEDIKRTLAYSSLGQIGYILLAFAAMDQAGWTTSL
                     YLALNHFLYKSMLFLAVAGVIQRTGTRNMYEMGGLIKKMPVSFLSVLMAIIALSGVPP
                     LSGFGGKWLLYSTLIGKGWYLEAAVAFFASGVAFLYLYRLIHSIFLGQPKPSQSEVRE
                     APAALVLPQLLLMAVLMAVSVFPRLMLDPILALVGDRFPTTLAFEGSRLRSALGYWDG
                     TLTMVVTGGVFALCLAWMLANLHNPQSVRQFNIVFAAERPLRPETTHYAWRFFEPYRK
                     ALGFLTRERAERFWDAFGTGAASVGGALRRLYTGNGQTYALHVVLYVAVLYLLLGVL"
     gene            21015..21929
                     /locus_tag="P5313_05185"
     CDS             21015..21929
                     /locus_tag="P5313_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRY79791.1"
                     /translation="METGILVRVGYALVSLFVITNYGLLLIGSMARISAKVQGRVGQP
                     VWQPYIDIVKSTAKRNSIGHGVMFYLGPVFRLAGGVGTYMFIPAVYGSVLFRNFSDSG
                     DLLLVMYFIFFGQLGMALGASEGGHPYSAIGVSRGLAQMTAFEVPFALSVIAVAIQYR
                     TLDITAIVAAQQGGFWNWTVATNPLAVAAALIAFLGMSMHNPFSIVIAPQEIPIGPPT
                     EYQSNLLGMLQTNRAIFNGAKLVLYMNLYFGGATDVVTMVVKTFLIYFINVFVGQAFP
                     RFRVDQSIRFFLGVPTLIGIASVIIAAI"
     gene            21940..22569
                     /gene="nuoB"
                     /locus_tag="P5313_05190"
     CDS             21940..22569
                     /gene="nuoB"
                     /locus_tag="P5313_05190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRY79792.1"
                     /translation="MSDKKGKPVPLANEGLPPGERGLFCDAPPREYAPAAKIVEDFWN
                     WARSQSLWILGFGTGCGAIELRPLMTSRYDMYRFGIQPRPTPRQSSVFVIGGYASVKT
                     LKRIVRSYEQMMGPKFVVALGSCTINGGMYWDSYNTIKRIDQYIPVDVYIAGCMPRPE
                     ALLAGFQELKRIIRAGKAEGQNLYARDFERYKANQKRVIKDWNMPDYNW"
     gene            22579..23112
                     /locus_tag="P5313_05195"
     CDS             22579..23112
                     /locus_tag="P5313_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRY79793.1"
                     /translation="MRELADMLSGRYGAREWHDQRKDLSSVTVDASKLRDVLTWLRDE
                     AGYAHLAFATAVDEIERKVFRLTYTVHNYGTAHGILVHCEIDRDRPVMESIHRLWGQA
                     WTYQREMREWYGIDFPGSPRVDEEFVLEGWEGPPMMRRDFDSLAYSDATYAERPGRET
                     RDPAAYMKEKLYPEADA"
     gene            23109..>23374
                     /locus_tag="P5313_05200"
     CDS             23109..>23374
                     /locus_tag="P5313_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRY79794.1"
                     /translation="MSRPKTPYVAPEERAVPDLDSGKYLVMWQGPNHPGITGNMSLRL
                     VIEGDSVVDCETHVGYLHRGFEKLMERRLWMQNFPLVCRIAVPEP"
BASE COUNT         3538 a         7964 c         7949 g         3923 t
ORIGIN      
        1 atcgcccaga aggtgaagat catcgtggag cactaccgcg agcacgtcgc gtggcggctg
       61 ggcggtcaag ccaaggccat ggtggtcacg aacagccgcg aggaagtgat ccgctacaag
      121 ctggcgatcg acgcctacat ccgggagaac aggtatccca tcgggacgct ggtcgtgttc
      181 tcgggcgaca tcaaggtcaa ggacctcgcg gaccttccct tcaacgagag caacatgaac
      241 cccggcctcc ggcgccggga catcaaggac gccttcgaca cgggggagtt ccagattctc
      301 ctcgtagcca acaaatatca gacgggcttc gaccagccga agctcgtcgc catgtacgtg
      361 gacaagaagc tatccggggt cgccaccgtc cagaccctct cgcggctcaa tcgaacctat
      421 cccggcaagg actggaccgc cgtcctggac ttcgtgaaca acccggattc catcctcgag
      481 gacttccagc tgttctaccg ctccgcgagc ctaccgcagg ggaccgaccc gaacctgctc
      541 tacaagctcg aaaccaagct cgacgcggcg ggcatctacc ttgagtcgga ggtcgaggca
      601 ttcgccaatt ggtactggga ccccaagcgc tccaagaaca accatttcca gcttcagaaa
      661 taccttcaac cgcccgtcca gcgcctcaag gagcggtatg tcgcagcgat tgaagggaag
      721 gacaacgaga ccaagaacgc gatcgagatc ttcgtgaagg acgtgggcag cttctgtaaa
      781 ctcttcgagt tcataaccca gattttcgac tacggcgacg acatcgggct ggagatgcgc
      841 tactcctact tcaaggagct gctgcccctc atgctcggaa ttctccggga cggacgagac
      901 tcaagcgaca tcgatctatc cggggtgcgc ctgtcccact acgggatccg ccccaaggac
      961 gcgggaagac ttcagctcga tccggccggc gtcagcgagc tcccgcctgc ctacggggac
     1021 atcgggtccg gaacgagccg ggatccggag accatcaagc tcctcgagct catcgaacag
     1081 ctcaacctaa tcttcgaggg ggacctctcc gattccgacc tcgtgaacta cgcgttcgcc
     1141 gtccgggaca agctcctgga gaacgagctt ttaagaaccc aggcccggaa caacacccgg
     1201 aaacagttcg acgagtccga cgacctgcac gaggccatcc tcgacgcggt ggacgagcag
     1261 gccaaggtga actccgacct cgcccggcag gtcatgagcg acgcccgcaa gcgcgacgag
     1321 ttcatgaaga tcatccggga actggcgtac tggggcttcc tgggcgagga gaaggcgggg
     1381 tgagcattct tcaacccaag acccgcccat gaccaacggg ctggatcaga ttttctccca
     1441 cagggccgcg aatccggccg ggtcttccag gggggccgcg tggcctccgt cgacctcgac
     1501 ccgtcgggcg ccgggaatcc cctcggccag gaaacggctg gcccggcggt tgtcgctcca
     1561 gtcccacagg ccgtcgacca ccagggcggg cgccttcacc tcggcgagcc gggacagggc
     1621 cggggattcc ggggcttggg gatcggaaag gaaaaaggtc cccctctcga cgaatatgcg
     1681 gttcatgacg gaagcctgtc gacgggcttc ccggatttcc ggggagaacc gagtcttgtc
     1741 cctgtcgggg ccgtcgaacc agatcctcag ctggagttcg gaggcggccg ccgtgtcccc
     1801 cgcctggacc gcggcgaaca tctccatcag gagcggaggc atgtcgccct ggggctgcca
     1861 gcccgacggc gaggcgttga ccagggttag ggacagcacc cggtccgggt gctccagggt
     1921 gaagtcgagg gcctgctggc cgccgttgga acagcccacg agatggaagg tctcgaggcc
     1981 cagggcgtcg actacggcgg tcagttcttt gcggcggacc acggggaccg cggcggggtc
     2041 ggaacggccg aaccccagct tgtcgtagga taccgtccga tggcgggccg ccagccgggc
     2101 tcggacgccg tcccacatcc gcccgtccaa aaagtcggca tgcacgaaca cgatggcggg
     2161 gccggtccct tcggattcgg cgtacatccg gccgtggccg ggaaggtcga gatacttgtt
     2221 catggtgact ccttgcgggt atattccacc cgggtgggtg acatcttgtg tcacccacct
     2281 tcgacatttt tcaaaaaaag aggatagcgt gcgagcagat cgactgattt ccctgatcct
     2341 ggccctgcag gaccggggaa agaccaccgc ggcgcatctg gccgccgccc tgggggtgag
     2401 ccgaaggacc atccttcggg acatcgacgc tctgtccctc tcgggagtgc ccgtgctggc
     2461 cgaaggcgga ctcggcggcg ggatctggct ggatccggcg taccgcacgt ccctgacggg
     2521 catgaaggag gccgagctgc ggaccctgct ggcggccgga gacgcccgcc tgctcgacga
     2581 cctgggctgg ggacagacct ttcgggagac ccaggccaag ctgagggcct cggtgccgcg
     2641 ccggttcgaa cccggggtgg agcacatgcg gcagcgccta atcatcgact cccggtggtg
     2701 gtggcacgag gccggggaac cgaacctcga ggttctgcag gccgccgtgt ggggagaccg
     2761 ggtgatcgag ggcgaatacc agacttggga aggcggcgcc aaggccgtcc ggctcgaggc
     2821 ctacggactg gccgccaagg cgggcctctg gtacttcgtg gggcgccggg caggcgagtg
     2881 gcgtgtttat cgtgtgtccc ggttcgaggt tgtccgagcg tcgggggcct tcgctcggga
     2941 tccgaagttc gacctcccgg cctggtggtc cgcccacgcc gccgaattcg cgggcgaact
     3001 ggcggggttt cggttctccc tgggccttcc cgaggatcgg gtgcgctacc tgcggggcat
     3061 cgccccggga cgcgtcgacg tggtcggacc ctgggagagg cccggctggg tccgggccgg
     3121 cgtggggctc gactcctcgc tctacgccga actcgtcgtc ttgggcctcg gacgccggtg
     3181 cgtgatcctc gacaacccgg agctggccga cgccgtggtc cggagatgcc gggaggggct
     3241 ggccgcccac gaaggattga cgtaacctgc gcgaagcatt agtcgacgtt ccccctgtcc
     3301 cattgttccg ggctccgggc cggtgtatcc tccctcactc ctccctcaag aacagaacgg
     3361 agctcgattg ctccgcggcc ctgccggtag agccggaccc ggccccgacg ctggcaaggt
     3421 acgcgaagtc ccggacccgg gcggggtcga tctccgccgc tcgttcgaag atggtccgtg
     3481 cctgggagaa atcggacaag gcgttgtagg ccagggacat gttcaccagg atcatctggg
     3541 ccgaggtgct ccggcccttg ccctgggtgt ccaaggcgcg gacggcgccc tgccaggaag
     3601 cgagcgccct gcggtagtcc ttctgcaggt atgccaggtt tcccaggttg acctgggcgc
     3661 tggtgaagga ggcatcgatc ctcagggccc gggtgaacgc ggactcggct tccttcgtcc
     3721 gtccgaactt cgcgtaggcg atgcccaggg agttccactc ccggacgtcg ttgcctttgg
     3781 ccgtctgggc cgcgagttcc gtgagggcga agtcgcccag ccggatgagc tccgggcgga
     3841 acgcggccgt caggtccgaa cgggaaccgt actggaggcc caggtcggtc tcgcgcaggc
     3901 cgaccggacg gtacacggtc tgggccgcgg cggtcgtgta gaaaccccgc ttgtccggat
     3961 tggtgtcata gagcttccat aactccgcgc cgcggcgcca ggcgtccgcg aagggctgct
     4021 tgccgaccag ggtgatctcg acgggtatcc agagccgatc gccctgcacg atggtcatgg
     4081 agcgatcggg atggatctcc cggaaatccc gggcgggtat tttcgtgttc accgccaggt
     4141 agatatgccc cggcaccgtg atgaagccgg tctcgtgtcc catcgattcc aggatgctgg
     4201 cgaagagcac ggtgaggtcg tcgcagtccc cggttccccg cgccagggtg gcccgcgcca
     4261 ggcttacgga gtcgaccgat atcgctcccg cctgggcccg cacgaagggc gagctggggt
     4321 ccgcctgata ggtaagcccg aaggacccca gggacgagta cacctgggcc gcggcctgca
     4381 tcgcctggtt gagctgggga agggtttcag ctctcaggga agtggccacg aagctgccgt
     4441 aattccggag tgccgagtcc gcgggggtga tgaaggctcc caccttgcgg tcgtcgtccc
     4501 aggtgatcgc ggtgcgatcg agcagctcgt aggacacggg caggcgctgt tccacgggtc
     4561 ggccgcgata ccggtagcgg acgatgacct ccccggccag gggtacggtg ccttccgtgc
     4621 ggaatacctc ctggttgaag gccgccagga gtccgatctc cgtacggccg cgcggtgcca
     4681 gggagtttag ggtcgcgctg ggggtcgggg cgtccatgta gccgggctgg aagaacgata
     4741 cctcgatatc ccggagttcg gtgttctccc cgttcacgag ggcgacctgg gcgaaggggc
     4801 gggtggaata atagctctgc atggccgcga acgccggagc gagtttctgg gtctccaggc
     4861 gcagggcgcg gacggccgcc gcggccgcgt ccgggtcctg gccgaagcgc caggagaccg
     4921 cgaagccgag gccgaagtac aggccgtcga aatcgtccag gaggccgagg cttcgggaat
     4981 accggatcgc gggtcgcagg tccacggaca ggttgtacga agggaccagc ccgatgcggg
     5041 ctccggcctc ggcgaggtat ccgagctcgc cgaccgtcgt gcccggggag aggttcggga
     5101 aggcggtctg gaagatcccg ccccccgcgt cgatcccgat ctcgaaatta cgggagaacc
     5161 gtgtggagta gcccaggccc agggcccccc acatctggct gtggtcgaat tgcgatcctc
     5221 ccgcgctgcc ggcctggagc accgataggg tccccgcgcg gacgaagggc tggacggtcg
     5281 acagattggt caggggcacg cgcagctccg ctccgatctc ccggaacgag aatccggaag
     5341 ggaagcctcc gaaggcctgc agatcccggt acatcgcccc caccgatacg gggaagctgt
     5401 accgctcgtt gagttccgcc atgctctcct ggggaaatac gggaaagatc gtcccgagcg
     5461 ccagcaccgc ggctatcgtg gagcgcgctc tcggtcttcc ttctgccctg cgatatccga
     5521 tcatgaccgc ctcctgattc atggcctgag actcacggtg tagagcttgc ttccgccgtc
     5581 cgcggcatac agatagtccc cgtagatggt caaggaacga ggttcgaaac cggccgcctg
     5641 gacggttccg acctggagcg gcgcaagggg gttgctgatc tcccagattc ccagccccgc
     5701 ggccccgtag gctacgtagg cgtagcttcc ccggaccgcg agatcgaagg cggggaccgc
     5761 ggaaggctgc ctccacgcgt gggccgttcc gccggcgctc caggcacggg ggttggaggc
     5821 ctcgtagacc aggagcccgc ccagggtgtc ggcggccagg acgtaatcgc cgtagacggc
     5881 cacggccgcg gtctcgcccg tgctccaggg ggacccgtac ccggccaggg cggaggggaa
     5941 ctccgggtcc tggacggaga ctgcacggac ccccgcggcc tcgttggcca cgaaggcgta
     6001 ggagccggcc agggctatgc gccgggcctc ccggttggcg ccgggggagg gctgggtggt
     6061 gacggccgcc acgacggtca ggaagccctg ggacgcccgg gacagatcca cgacgttgag
     6121 ccaggagttc gaggttccca tcagggcgta gttcccgaac agggccacgg accacgcgtt
     6181 gcctccctgg gtgctgacgt accagggagg aaggacctgc ggggcatggg tcggggctcc
     6241 cggcgtctgg agcgaccact gcatcacccc gctccgctcg gcggcgatgt aggaacgctc
     6301 ccccgcgaag gagaagtcat agggagtgta gcccccggcg tcggtggcca cccgggcggg
     6361 taccgcgggg gcttccaggc tgaatacgga cgaggtccag tccgccgagt attccgacac
     6421 gtacagtctt cccccgaacg cggcgagccg ggcggcggaa cgcaggccgg tctgggtatt
     6481 caccaggacg gccgcggacg gatcgttggc gccgtagata cggaacggat aatcccggcc
     6541 cgtggacacg aaggaatatc cgtctcccac ggccacgtgc tcggtggagc ccgggacgtt
     6601 ccgggtgaag gcggttcgca gggacgaggg attcgagaca tccaccaccg aatacccgta
     6661 gatgttcatg gccgcgaata ctcggttccc ggacacggtc aattctccga cgagcacgga
     6721 ggacccgccc ggttcaaccg cggatagcgc ggagggcgag gccgggttcg aggtctgcca
     6781 ggcccggacg aagctgccct gggcggcgta caggaccgtg ccgctcgccg tgaaggcgac
     6841 cgaacccgcc ccgcttatgg aggaactgtt gcgtgtgacc gtcgggacgg cgtcggttcc
     6901 cgtgaccgta tagacggcgg tcgcgctgtt tccggccacc gccacgagac gggttccgga
     6961 acggttctgc accgtcacgt cgtaggcctg gcccaggagg gccgcgtccc gagcctgaat
     7021 gcgcacggcc ggagccgact tgttcgtgac gtcgaccacg tagagcccgc tgttgaatcc
     7081 cgccacgaag gccaggtttc ccaggacggc cacgctttcg ggttgaagaa cgaaccccga
     7141 gggatccagg tccgcatagc ccgcgatcga gggcatgctc ggatttccta tgtctacgat
     7201 gaacagggcg atcgcccatg ccgttccggt ccaccggtag cccccgagat aggcgtacgg
     7261 ttcatcctcg tccagtacga cggattggac ctgggtcagc gcgaggaatc ccgcctgcgt
     7321 aggcagtccg gggttccgta cgtccaagac ccgtaaccct ccgtaaaagt ccgcgatgta
     7381 caggtacccg tccttgtagg ccgagctctg ggaattttcc accccgtcga agacggccac
     7441 cggggcgtcc tggcgggacg acacggagtc ggaggccgcc tcgatgtact cgctcacgac
     7501 cgtgcttcgg gatgtcggct tgacggcgta gtagtatctc cggcccccgg aggcgctggc
     7561 gtcgaaatac cccgagccca cgaccggccc cgagacgtcc tcccacgggc ccgccggcga
     7621 cgcggaccgg agcacggtat aggagggtat ggactcgaag gtccgccagg agagggctat
     7681 gccgtcgtag cgctcggtcg cccggggcag aagatgcatc cgggacaggg gaacggagcg
     7741 aacgacctgg gagtagttca cgcggccgcc cgggccgtcg gcggccagga gcaggctgta
     7801 gacggaaccg ttggcaagct ggttcaaggt gtgcggtgag gtgacgtcgt cgaaataggt
     7861 ggagatcccc gggtccggag tcttgctgga gggttcgacg tagagccggt accgggtcgc
     7921 gcccggcacc gggtcccagg tcaaggtcac cgaccggctg tcctcctccg actggaagct
     7981 cgggatgtcg ttccccgggt ataccagcgg aagccggtat tccgccgcgt ttccgttcca
     8041 gtccacggct ttcaggagga cggttatgtt cgtgcgcagg tcgttgggct cctcgatcac
     8101 gaaggccccg gagggcgata cggtcgcgga cttttcggcg gtgtacgaaa gtatctcgta
     8161 gctcagggac gatacccgtc cggagcggcc tccgccggcc ccgtccagta cgaccccctc
     8221 gatccggatg accctggaat agctcgaata ctctgaaggc gtcgtcacga ggatcgacgg
     8281 ggattccgca tccttggcgc tgagcacgat gtcgtccgtg atgggattcg ggcagccggt
     8341 caggattacg accaggacgg cggcagcgag ggtgaaaggc cgcgaagctc gggacatgtc
     8401 ccacctcctc cgggagaaga gcagatggag ctattatatt actttttttg tcatttataa
     8461 aggacgcaag gaaaattatc ggagcgaaag aaatccggac acgtccgccc ggccgtgtcg
     8521 aaccgtgaca cctccccgaa aaagcagtat ccttaaaaag ccaacaacgg aggtgatctc
     8581 catgaaccgg aaaaagtatc ctattctgct gtccgtcctg cttgccgccc tggtcgtcct
     8641 ttccgcctgc agtcccaagc cccccgtcac cccggtcacc gtagccacca tggtggactc
     8701 cgaaggggcc atcctgggca aggggatgat cctgcttatg gaagccaagg gcatcccggt
     8761 gaccgacaag gtcgggttcg ggaccccgga catcctgcgc aaggccctgg tggccggaga
     8821 ggtggacctg gtggtggact acacgggctc ggggcagtac taccacgagg acctggacac
     8881 gacggtcttc aacgatccgg tcaagggcta cgagttgatc cgggatacgg acaggaagcg
     8941 gcacgacctg gtctggctgg cccccgccaa ggcgaacaac acggaggccc tggcagtgct
     9001 acggagtttc ggccaggaga acggcctgcg gtcgatgaag gatctggccg cctacgtgaa
     9061 cgcgggtaaa ccggtcaagc tgatctgcgc ggtcgggttc gcggagaatc ccctgggcct
     9121 gcccgggttc gagaaggcct acggcttcaa gctccggaag gaccagctga tcctcctgga
     9181 aagcgggaac accgcccaga tgctgaaggc cctggtggac ggcacggacg gggtgaacgt
     9241 ctccctggtg tacgggaccg acggcgccct ggacaagatg aacgtcctgg tgctggagga
     9301 tccggcgaac gtgccccccg tgtacctccc gacccccatt ttgcggggag cggtccttga
     9361 aaagcatccg gccctgaagg acctgttcac ggccttcttc gagtccctgg acctggtcac
     9421 cctccagcgc ctgaacgccc gggtggcctt cgacggggag gatcccaagg aagtggcgcg
     9481 gtcctacctc aaggaacgcg gcctcctgaa gaactgacgg cccccggacg gatccgcacg
     9541 gcatgaccgt cgactggata gccctggcgg gggccggggc gggactggtg tccgccgccg
     9601 ccctcccctt cctcagcctg cgccccaacc ggatcctggc cggggttccc cagtacgcga
     9661 tggaggttct gggtgccggg tcggtcctga tcttcactgc ggcggcagcc gccgcggtcc
     9721 tcgcggccct ccgaagacga cactccgggc tcgacgccgc gggaaccctg gtgtccggcg
     9781 cgggcctggc ctcccttctg cccttggcgg gagccgccgc ggaccgcctg gcgggggatt
     9841 ccgcggtcgc ccgggtatcc gcgggccccg gactctggct ggcggccgcg ggtcttggag
     9901 cagccctctt ttccttctcc cgcaggcttc cctcccggtc ggcgctgcgt ctggccgcgg
     9961 cgctcgcgat acccgcggcc gcggcggccg cgggccttgc cggcctcctg gacgatctct
    10021 ccctggtccg ggaactcctt tcccgccggg acaccttcac cgccgagctt gtccggcacc
    10081 tggccctggc cggaggatcc gcggccctgg gcgcggcggt cggcatcccc ttgggcttgg
    10141 ccatgcaccg ccgcccccga tggcgggggc tcatctccgt ggcggtcagc ggggcccagg
    10201 tcatccccac cctttccctc ctgggcctgc tgatcgtgcc gatggccgcc ctgggcgcgg
    10261 cggtgcccta cctgggccgg atcggcgtgc ggggagtggg atgggcgccc gccttcgtcg
    10321 tcctcttcct gtacgccctc ctgcccatcg cgggcaatac cttcgcggcc ctgggggccg
    10381 tcgcgccgga agccctggac gcggcccggg gcatggggat gagccgtcgc caggccttcc
    10441 tgcgggtcca ggcgcccctg gccctcccgg tcatcctggc gggggtccgc accgccttca
    10501 cggccaatat gggaaacgcg atcctggccg ccctggtggg cggggggggc ctcgggaccc
    10561 tggtcttcct ggggctggcc caggcggccc cggacctgat cctcctgggc gccctgtcca
    10621 catcgaccct ggccctggcg gcggaccggg ccctggccct ggccttccgg cttgcggtat
    10681 cccggggcct gcgatcggag gctccgtgat ccggttccgg aatatcgtaa aatcctacgg
    10741 atccgcccgg gccgtggacg gggcggacct cgagatcgcc ccgggggagc tctgcgtcct
    10801 catcggacct tcgggctgcg gcaaatccac cctcctccgg accgcgaacc ggatggtgga
    10861 acccgactcc ggcgaggtgt tcgtggacgg ccggcccgta cgggaacatc cgcccgagga
    10921 actgcgcagg gggatcggct acgtcatcca gtcggtgggc ttgtttcccc acatgaccgt
    10981 gggggacaac gtggccgtgg tgccccgcct cctgggcata gacaagggcg agatcgccgg
    11041 ccgggtcgac cggatgctcg agctggtggg cctttctccc gagtggtcca gccgctggcc
    11101 ccgggaactg tccggcgggg aggcccagcg ggtcggggtg gcccgggccc tggccgcgga
    11161 tccacccatc ctgctcatgg acgagccctt cggttccgtg gaccccctga cccgggtccg
    11221 gctccagcgg gagttcctgg ccatccagcg ccggctccac aagaccgtgc tcttcgtgac
    11281 ccacgatgtc gaggaggcca tccgcctggc ggaccgcatc gccgtcatgc gttcgggacg
    11341 gatcctccgg catgccaccc ccgaggagtt cgtcgcaacc gacgcggaag acttcgtggg
    11401 agaattcctg ggcctggagt acggggtgga gatcctatcg cgccgtcgcg tccgggaagt
    11461 cctgcggccc gctgcggaga tcggctccga tcccggcgcg ccgtccatcc gcgccgaggc
    11521 gagcctcaag gaggccctgg gactcatggc cgcccggagg gtcgaatgcc tttccgtcac
    11581 cgacggggaa ggccgccggg ccgggagcct ccgcatggag gatgtcctgg ggacctggaa
    11641 ggcctgcccg tgaacgatgc ctccggaaat cccgcggccg cccaggccga ctcccggcgc
    11701 ctccgggctc cgggctgggt ctttccggcc ctgctcctgg ccgtcctgat cctctcggtc
    11761 ctccgtttcg acctaatcga gtccctcctg ggaatccgag cccggggact attggtttcc
    11821 cggatccccc tctgggaact ggccctccag cacctgggcc tggtggccgc ctccagcgcc
    11881 gcggcggtgc cgatagccct gggcctgggg atcgctgcgg tccggttttc ggaatccggt
    11941 ctccgggccc tgctggtcgc ggcgggctcc gcggctcaga cctttccgtc cgcggccgtc
    12001 ctggccctgt cggtccccct ggcgggcttc ggcttcgccc cgaccttcct ggccctgagt
    12061 ctgtacggga tgctgccggt cctgcacaat acgatcgcgg gattggaagg cgtcccggag
    12121 gcggcggtgg acgccgcccg gggcatgggc atgcggcccc gccaggtcct gatccgggtc
    12181 caactccccc tggcccttcc cgtgatcctg gcgggggtgc gcaccagcgt ggtgatcaac
    12241 acggcggcgg ccaccctggg ggccgccatg ggtgccgggg gcctgggcct ccccatcatc
    12301 agcggtatcc ggagcttcga tcccgccctc atcctgcggg gagccgtacc ctccgccctg
    12361 ctggccctgt tcgcggacgc gtccctccgc cgcctggaag accgggcgcg gcgggccgcg
    12421 ggagggaaac cggacggtcg gggagcctga gccgggccct ggcggagaat cccgaacccc
    12481 gcgcgcgatc tgctcaaagc atccccgaag ctcccgtctt ccccgccccc agcaccgccc
    12541 gacgcaggag cttcgggtag gtcttgaagg cgaacccttc gtggatgcgc tcggagagct
    12601 tgtgggcgtc cttgcccacg ggacccaggt tgaggacggg aacctccagg cgatccaggg
    12661 cctccagggg tagttcgtag agcggaccat acccggggac gttttccgta aaggccgcga
    12721 ggtcggcctt cccgcagggt gcggccaggt agctcaggtc cgagatgccc tcgtagtagg
    12781 gccggatcga gaggggcgat cctagggacg cggcgaagga tcgcaggtca cgggccaggg
    12841 cgagcatggc ccgatcgcct acggaccggc cgtcgttccc gcggtggggg taccagggca
    12901 tgaggtaccc gatgaccacg agggggccct tctcccccgc cagggtgacg caggcgtcca
    12961 cccgggccag ggcccggtcc cgggcgtccg agccgggcgg cagggcgttc ccggcggcct
    13021 cgaccgcggc ttcccagtcc ggtcccgcgg cggcccgggc ccggccttcc agttcggcgt
    13081 acgtgagcac ccggggctcc agatccaaag cggcgggccg gggcttgccc atccgggaaa
    13141 agcggcgggc gttcttccgg gtccgggcga tcgaaaggcg cagggccgtt cccgcgatgc
    13201 ggcgcatctc ctccaacagc cgtcccggca gggtttccgc ggtgaagtag ctgtagtacg
    13261 aggccgagcg ctcgggcacg gagcaggaat actcgtgccg gaagtcccgg tgcttcatgc
    13321 aggcccaggg ggtcagggcc tcccttcccc cgcggtcggc ccgggcggga tcgccctcga
    13381 tcagaaggtt gatctgggat gtgaggaggg cggcggacag gccctcgtag tactccccca
    13441 ggtgggcttc ccggccgacg cagaagaaga agggattgat cttgcccgcg gaacccgtgc
    13501 agaccgcggc caggtccgaa agtcggtccc cggtcccgat ggaaggctcc gtgttgatgc
    13561 aggccaagaa acgaaggccc ccgtcctgca ggcgcgccag gtgccggacc gcccccagca
    13621 tgccccggga ggagtcctcc tcgtccggga cgaagaggag agccaggttg gtttccaggg
    13681 ccgcgtgttc ctccgcgaag tcctccagca gggccgtcgc gacggccgcc ccgcacttca
    13741 tgtccgcgac gccccgcccg aagagccact ccccggactc caggtcccgc cgggcggagg
    13801 gggacagggg catcccgccg atccgggtgc cgtagccctc ggggtcgaag gcgtacggag
    13861 ccagggggcc gcagaccttg accccgacca cgtcgatgtg tcccgtgagg atcaccgtat
    13921 cccgcaggcc cggccggggc cggagcaggg cgaagaggaa ggaccgtccc aggggatccc
    13981 cctcgcaggg caggagggcc aggtcctcgg gatggtcccg gaaccaggaa agcatggtca
    14041 ggcggtcccg aaggtaacgg accgcggcgt cctcggtccg ggggtccggg gagacggagg
    14101 gtatggcgac gaggtccagc aacagtctac ggatgctttc ggggctatgg ggcatggcga
    14161 gaactcctgg tcgcggctgc ccgccgcggc tccaggatgg cgtccccggc ggagtcccgt
    14221 caaggaaggc gccggcggtt ccccgagcga ctcccggcgc ccccggaagc gcggcggcga
    14281 tccggaccct cgggcgactt ccgaatattc tacgctcggc aatgtatttt tattgttctg
    14341 cgatatcttc ttattgtttt gcgatatgtg gatcatggct gcccggaagg catgcccgca
    14401 cgcgtggatt ctcccgggta taaactatcc catgattcga caggaaggga accggtatga
    14461 acagagtttg gatgatcgcc gtctgtatcg cgatcggggc gggcgggctt tctgcccagc
    14521 ccgcatggca gaagcatatg ggggaagagc cggtatcgga attctccctt tccggtccgt
    14581 acgccaaggc ggaactcggg gcgatccgga cggagctcgg ggatcgggag ttcggggagc
    14641 tgaccctggc ccggctggag ccctactgga ctcggatgaa cctggccctg agcaaggacc
    14701 agtaccttga gcgtgtctcg aagatgtcca tgatccttcc gggaaccggg cagttcgaga
    14761 acggcgacac gtcgaagggg gtcggattcc tgtccctcca tatcgccgtg gtgaccggta
    14821 ccctggccgg tttctacttc ctgctccccg ccgacctgag gttcgatcgg ctcgattact
    14881 tcaagacctc gttcaaggac atcaacgaca cgtgggaggc gcacagcctg aacgactatc
    14941 tcccgtcgat cgggatgatg ctggcgggaa cccttgtcga tctgggcgta cggttctggg
    15001 cctccggcga cgcgtatgcc ggagcccggg ccgccgtgga ttccggccgg gccgagctga
    15061 aacccgtggt cggccccgga tttctgggag tcggaatgtc ctactgatcg ttccgcgacg
    15121 ccgcccccgc cgggcggagt gtccaaaaag ccaactgggc ttttaggaca gcccgtccgg
    15181 tacccgcgct tgtgccggaa gaaataaacc cggaaatccg ggcaatccgg tccccgcatc
    15241 cactatagtt gtttccatgc gtccgcccgc cgatcccctg cggggccctc ggcggccggc
    15301 agggggcgcg gccgagcgcc cctgcggacc gccgtccgtc ttcacgctcc tccccgcccg
    15361 ccgaaattcc gggaaaaaaa tatatcaaca tctatatttt gctttacata tattgaatac
    15421 ctcatatatc atgtcgaaaa ccgccccggt cccggggacg gccggtacgg agggctttcc
    15481 atgcgaacag tgaaacaacg gatcctgtcc ttcctcgggc tctggggcgt ctggctcctc
    15541 ctcacggccc cctggaattc cgaggaggcc gcggccgggg ccgtcgtcgc cctcttggtc
    15601 gcggcgctcc ccttctccgc cgcctccccc ctcggggacc tgaggctggg cccccgggcc
    15661 ctggcgaccc tggtcgccta tttcttcgtc ttcgccaagg ccctggtcct gtccaacctg
    15721 gacgtcgcct tccgggtcct ccacccccgg ctccccatcg ccccggggat cgtgaaggtg
    15781 aagacccggc tcaagacccc cctcggccgt ctcctgctgg ccaactccat caccctgacc
    15841 cccgggacga tcacggtgga gatgcggggt gaggatatct acgtccactg gatcaaggtg
    15901 gcggactcgg atccggaagg agccaccgcc gccatcgtgg ggggattcga gaaatacctg
    15961 gaggtgatct gtggctgaca ccgtcttcgc ggcggcttcc gggctcgccg tcctgggcct
    16021 cctcctctcc gccgtccgct tcgcccgggg gccctgggcg atcgaccgga ccgtggccct
    16081 ggacgtgatg accatcatcg ccacgtcctt gatcgtcgcc ttcgccgttt tctccggaag
    16141 gaggatctac gtggacgtgg ggatggtcta cggactcgtg ggattcgcgg gggtcgtggc
    16201 cgtcgcccgg tacctggaag gggggctctg agatgaacga gaccatcggg gctttcgtga
    16261 gccttgccgg ctccctcttc ttcctccttt cggccatcgg cctcctgcgg atgcccgacg
    16321 ccttcaaccg gatgcaggcg ggcaccaagg cgaccaccct gggttccatc ctcttcctcc
    16381 tgggcgtcgg catcatgaag ccggactggc tgggaagaat gatcatcctg atcgtgttca
    16441 tcgtcctcac gaacccggtc agctccaacg ccctggcccg ggcggcccac gcccatcgga
    16501 agcggatcgg agcccggctg gaaccggacg ccctcgcggc cgcggacgag gggaaggagg
    16561 ccggacatgc tggttgacgc cgccatcctg gtttccggct tcgtcatgat cgccgcgggg
    16621 atcggggccg tcctggcccg gagcctcctg gtatccatcc tcctgtccgg aggggtgagc
    16681 ctcgtggcca gcctcgtgtt cctcctcatg ggggccccgg acgtcgccat gaccgaggcc
    16741 gccatcgggt ccgggctgac caccgtggtc tacctctacg cgatggcccg ggcccgacag
    16801 cccgacgagg aggacgcgga tcccctcggg gatgccgtga agggaggccg tcgatgatgc
    16861 gccgtgtcct ggtgttcctg gccgtcgccg gtctggccgc ggccctcctg cccctggcct
    16921 tggggacggg ggtccccgag gcccccggcg ccctggcgac cgcctacctg gaggacgcgc
    16981 ctggggactt cggggccgcc aacgtcgtga cggccatcgt ggtgagctac cggggcttcg
    17041 acaccctggg cgaggtggcg gtcctcttcg cggccaccgc cggggtgggc gccctcctca
    17101 cccggaagcg cctgggcatc ggggacgcgg agcccgcgga caaacccgcg gccacggaga
    17161 tcctggtcac cgccacgggg ctcctcacgg gcctcctggt gatgttcggg gtctacatat
    17221 tcctgcacgg ccacctcact cccggcggcg ggttccaggg cggcgtggtg atcgccacgg
    17281 cccttctgct cggactcctg gcccggccgg aatccaaact ctcccacggg atcctgtcgg
    17341 cggtggagtc cctgtccgga gccgcctacg tgggcatggg cctggcgggc gccttcctgg
    17401 cctggggttt cctggacccc cgggtcctgc ccctgggccg gatcggatcc ctggtcagcg
    17461 ccggatccat cccggtcatc tactccctga tcggcctcaa ggtcggcgcc gagctctccg
    17521 gaatcctcga cgccttgaaa ggagaccgcg aatgagcatc ctggaattcg tctcccggat
    17581 caaccccgtg gcggcctccg gggccgtcct ggtgctggtg ggcgtctggg gcgtcctggc
    17641 ccggccgaac ctcttcaaga tcgccatcgc gacctccgtg ctggagaccg gcgtgaacct
    17701 ggtcatcctc tcaaccggct acatccgggg ccgcacggcg cccatcctgg acagcgcagc
    17761 ggacgcctcg acggccgcgg cccggatgac ggaccccgtg ccccaggccc tggtcctcac
    17821 ggcgatcgtc atcggtctgg cggtcacggc cctccagctc tcctacgccg tgcggctccg
    17881 ggccgccggg gggggctcct ccatcgaatc ctacggagac gagaaatggt gaaccccctc
    17941 tggctcttcg ccctgtccct gggggcggcc ttcctcctgc ccctggtgga ccgcctgggg
    18001 cgcaggatcg ccctggccct ggtccacggg gtgacagccg cctggctggc ggccggcatc
    18061 ttctggctat cgatcctcct gtccggcggc gaggccatgg acttcgggac cgccgggttc
    18121 atggctcccc tggccatcgt gctccgggtg ggccttgccg aggcggccgg ggtgacggcg
    18181 gtcgccctga cgggcctcct ggccgcctcc tccctgaccc ggtccctccg ggacgcccca
    18241 ctggggggat ccatggccct cctcatggtc ctcatggggc tctccgggat cgtcctcacc
    18301 cgggacctct tcaacctctt cgtgttcctg gagatctccg cgatcgggac ctacgggttc
    18361 ctcgggttcg acgaccggaa gccggcgctt cgcgcggcct tcaagtacat gatcgccggg
    18421 ggactggcga gctccctgta cctgatcggg gtgatctacg tgtaccgcct cgcggacacc
    18481 ctgaacctgg acatcgccgc ctcgggcacg gccctctcgg gccccgcagg gaccgccgcg
    18541 gtcttcttcc tcctggccgc cctcctggtg gagctcaagc ccttcccggc caacggttgg
    18601 gcggtggacg cctacgagac cgcccatccc ggggtttccg cgatcctctc cggtgcctcc
    18661 gccacggccc tcctcctggc cctctggaag atccttcccc tcctgggccc cgcccacctg
    18721 tccctggtgg ccgcgagcgg actggccacc ttcgcggcgg gcaacctcgt gggagcccgg
    18781 caggaccggc ccctccgtat gctgggctat tcctccgtgg cccaggtggg cctggtggcc
    18841 gccgtcgcgg ccctcgcccg gctctcgggc ctgggggacg ggggatcgga cttcgccgcc
    18901 atggtggcgg gcgggctcct cctgaatcat ctcctggcca agacgggcct cttcctcctg
    18961 gccggggccg tggaggacga tcgcgcccgg ggacccctgt cccccctggc cctggccgcc
    19021 gcgggcatcc tttcggcggc cctggtggga cttccgccct tcccgggctt ctgggccaag
    19081 tggcgcctgg tccgagccct ggcggactcg gggcggtggt ggatcctggc cctggtcctg
    19141 ggagggagcc tgctggaggc tttctacgtc ttccggtggc tcggtgcgcg taccaagggc
    19201 gaggccggcg aggcacccgt ccggctcggg atccggggcg ccggccccgt cctggcggcc
    19261 ctgctcctgg tcgccgcggg cacgctgggc gggatccggg gaccggtccc ggacctcctg
    19321 ctctgggccc cctgggccgc ggccgccgtc ctcttcctcc tggacggaat ccccggccgg
    19381 ctcaagggca tcctgtccgc ggcggccgcg gccgccttcg gcgcggccgt ctggggccgt
    19441 ctggacggct tggggcagtt cttcgcggtg atgctcctgg gcggcggagc cctcttctcg
    19501 atagcggccc tccgcaaggg cggcgcccgg cggggccttt accccctcct gtccgccgcc
    19561 accctggccc tcggggccat cctcgccgct aggaccccgt tggagttctt cttcgcctgg
    19621 gagaccatga cggccgccag ctggctcctg gtcctgcggg gccgggacgg gcggaagccc
    19681 gccctgtcct acatcgtctt cggcctgggc ggtgccttcc tcctcatggc cggcctggcc
    19741 gcggggggct gggacggtgg gaccgcggcc gcgggctggc cgggcctcct ggtcctggcg
    19801 ggcctcctgg tgaaggccgg ggccctgggg gtccacgtct ggctgccggg ctcctacgcg
    19861 gagtcggacg acgagtcctc gggcttcctg tccgcggccc tctccaaggc cccggtcttc
    19921 gccctcttcg ccctggccct ctccctggga gccgccctgc ccggcggacc gtggactagt
    19981 cgggtcctgg gttggctggg cctggccacc gccctggggg gcgccctgta cgcggtctac
    20041 caggaggaca tcaagcggac cctggcctac tcctccctgg ggcagatcgg gtacatcctc
    20101 ctggccttcg ccgcgatgga ccaggcgggg tggacgacct ccctctacct ggccttgaac
    20161 cacttcctgt acaagtccat gctcttcctg gccgtggcgg gggtgatcca gcggaccggc
    20221 acccggaaca tgtacgagat gggggggctc atcaagaaga tgcccgtgag cttcctgtcc
    20281 gtgctcatgg ccatcatcgc cctgtccggg gtgccgcccc tgtccggctt cggcgggaag
    20341 tggctcctct actcgaccct gatcggcaag ggctggtacc ttgaggccgc ggtcgccttc
    20401 ttcgccagcg gcgtcgcctt cctctacctc taccggctga tccactccat cttcctgggc
    20461 cagcccaagc cctcccagtc ggaggtccgg gaggccccgg cggccctggt cctgccccag
    20521 ctgctcctga tggcggtcct gatggcggtc tcggtcttcc cccgcctgat gctggatccc
    20581 atcctggccc tggtgggaga ccggttcccg accaccctgg ccttcgaagg gagccgcctg
    20641 cgctcggcct tggggtactg ggacgggacc ctgaccatgg tcgtgacggg cggggtcttc
    20701 gccctctgcc tggcctggat gctggccaac ctccacaacc cccagtccgt gaggcagttc
    20761 aacatcgttt tcgcggcgga gcgccccttg aggcccgaga ccacccacta cgcctggcga
    20821 ttcttcgagc cctaccggaa ggccctgggc ttcctgaccc gggaacgggc cgagcgcttc
    20881 tgggacgcct tcggcaccgg ggccgcctcc gtcgggggag ccctgcggcg cctgtatacc
    20941 ggaaacggcc agacctacgc cctccacgta gtgctgtacg tggcggtcct ctatctcctc
    21001 ctgggggtcc tgtgatggaa accggaatcc tagtccgcgt cgggtacgcc ctggtctccc
    21061 tgttcgtcat cacgaactac ggcctcctct tgatcgggtc gatggcccgg atcagcgcca
    21121 aggtccaggg gcgcgtgggc cagcccgtgt ggcagcccta catcgacatc gtgaagagca
    21181 cggccaagcg gaactccatc ggccacgggg tgatgttcta cctgggtccc gtcttccgcc
    21241 tggccggggg ggtgggcacg tacatgttca tccccgcggt ctacggctcg gtcctcttcc
    21301 ggaacttctc ggactccggg gacctgctcc tcgtgatgta cttcatcttc ttcggccagc
    21361 tgggcatggc cctgggggcc agcgaggggg gacatcccta ctccgccatc ggcgtgagcc
    21421 gggggctggc ccagatgacc gccttcgagg tgcccttcgc cctgtccgtg atcgccgtgg
    21481 cgatccagta ccggaccctg gacatcacgg ccatcgtggc cgcccagcag gggggcttct
    21541 ggaactggac ggtcgcgacg aaccccctgg cggtagccgc cgccctgatc gccttcctgg
    21601 gcatgtccat gcacaacccc ttcagcatcg tcatcgcccc ccaggagatc cccatcggcc
    21661 cgcccacgga gtaccagagc aacctcctgg ggatgctcca gacgaaccgg gccatcttca
    21721 acggggccaa gctcgtcctg tacatgaacc tctacttcgg gggcgcgacg gacgtcgtga
    21781 ccatggtggt gaagaccttc ctcatctact tcatcaacgt gttcgtcgga caggccttcc
    21841 cccggttccg ggtcgaccag tcgatccggt tcttcctggg cgtgcccacc ctgatcggca
    21901 ttgcctcggt catcatcgcg gcgatatagg aggccgaccg tgtccgacaa gaaaggcaag
    21961 ccggttcccc tggccaacga gggcctgccc cccggagagc gggggctgtt ctgcgacgcg
    22021 cccccccggg agtacgcccc ggccgcgaag atcgtggagg atttctggaa ctgggcccgg
    22081 tcccagagcc tgtggatcct gggcttcgga acgggctgcg gggccatcga actgcgcccc
    22141 ctcatgacaa gccgctacga catgtaccgg ttcggcatcc agccccggcc gacgccgcgg
    22201 cagtcctcgg tcttcgtgat cggaggctac gcctccgtga agaccctgaa gcgcatcgtc
    22261 cgcagctacg agcagatgat gggcccgaag ttcgtcgtgg ccctgggatc ctgcaccatc
    22321 aacggcggga tgtactggga cagctataac accatcaagc ggatcgacca gtacatcccc
    22381 gtggacgtct acatcgcggg ctgcatgccc cggcccgagg ccctgctggc gggcttccag
    22441 gagctcaagc ggatcatccg cgccgggaag gcggagggac agaacctcta cgcccgtgac
    22501 ttcgagcggt acaaggccaa ccagaagcgg gtcatcaagg actggaacat gccggactac
    22561 aactggtagg ggggaaggat gcgtgaactg gcggatatgc tttccggccg ctacggcgcc
    22621 cgggaatggc acgaccagcg gaaggacctc tcctccgtga ccgtggacgc ctcgaagctt
    22681 cgggacgtcc tcacctggct ccgggacgag gcgggctacg cccacctggc cttcgcgacc
    22741 gcggtggacg agatcgagcg gaaggtcttc cggctgacct acacggtgca caattacggc
    22801 acggcccacg ggatcctggt ccactgcgag atcgaccggg accggcccgt gatggaatcc
    22861 atccatcggc tctgggggca agcctggacc taccagcggg agatgaggga gtggtacggg
    22921 atcgacttcc ccggcagccc ccgggtggac gaggagttcg tcctggaagg ctgggagggg
    22981 ccgcccatga tgcggaggga cttcgattcc ctggcctaca gcgacgccac ctacgcggag
    23041 cggcccggcc gcgagacccg ggaccccgcg gcgtacatga aggaaaagct ctacccggag
    23101 gccgacgcat gagccgcccc aagacgccct acgtggcccc cgaggagcgg gccgtcccgg
    23161 acctggattc cggcaagtac ctggtgatgt ggcagggtcc gaaccacccg ggtatcacgg
    23221 ggaacatgtc cctgcgcctg gtgatcgagg gcgactcggt ggtggactgc gagacccacg
    23281 tgggctacct ccaccgaggc ttcgagaagc tcatggagcg gcggctgtgg atgcagaact
    23341 tccccctggt ctgccgcatc gccgtgccgg agcc
//
LOCUS       DAOTEI010000035        22793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1480_length_22793_cov_5.503562, whole genome shotgun sequence.
ACCESSION   DAOTEI010000035 DAOTEI010000000
VERSION     DAOTEI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 22793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22793
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_22793_cov_5.503562"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..427
                     /gene="rpsI"
                     /locus_tag="P5313_05205"
     CDS             35..427
                     /gene="rpsI"
                     /locus_tag="P5313_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRY79795.1"
                     /translation="MVRNLGIGTGRRKTSVARVYLREGTGKILVNGLEADKYFRFEEL
                     FNKVRGPLVVTANENKLDVVINVVGGGMNGQAGACRHGLARALAQFDPANHTALRTNG
                     MLSRDPRMVERKKYGQRGARRRFQFSKR"
     gene            443..1063
                     /locus_tag="P5313_05210"
     CDS             443..1063
                     /locus_tag="P5313_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRY79796.1"
                     /translation="MRVESLEIGASGIVRLRTDGGSCFLFRLEQLSPSAGPVPSIGQE
                     AGDTFLAGAKEASGIYAAETKALELLARAEQTRYLLTLKLRKRGMGDGSISPALDRLE
                     GQGLLSDERFARAWAEERARRRGEGPARIAAGLAARGVSEDLARRVCVELYPEEARGE
                     ALLRAARRLLRSGRKDPEAAARKLRSEGWKPSEIRGALEAIRKEGD"
     gene            1137..1793
                     /locus_tag="P5313_05215"
     CDS             1137..1793
                     /locus_tag="P5313_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY79797.1"
                     /translation="MDDDILTIEEVARYLRVSERTVYDWAQKGEIPSGKIGTVWRFKK
                     SEIERWVNERLSSNRSSAASNPVLIRNVLTPERIIFPNHPHKRDALVALAETLATAPQ
                     VKSRPELVSEILRREELMSTAIGRGIAIPHVRLSSVTDLVVAVGISKCDIVDFASFDD
                     SPVRILFMIASAYNQHAYYLQTLSYFSSKLKNAELRETLLKATEPQAVYDIFAKSDEA
                     "
     gene            1916..2221
                     /locus_tag="P5313_05220"
     CDS             1916..2221
                     /locus_tag="P5313_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY79798.1"
                     /translation="MPTYEYECSSCGHTFEAFQSMSDAPLSSCPECGKAVKRLIFGGT
                     GVIFKGSGFYVNDSRKSSSGSPKAGAPAAKDGAVKDVPAKEGAAKDSPAPAKAQESA"
     gene            2256..2558
                     /locus_tag="P5313_05225"
     CDS             2256..2558
                     /locus_tag="P5313_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79799.1"
                     /translation="MKRPRYFCEHCGAEVGRDASVCPKCGRFFSSVKCPRCGFSGEPF
                     LFRNGCPTCGYSAASGSPAAPKSGRPTASGPLPGWIYAAAFMALAAALAAVLIAAR"
     gene            complement(2638..3174)
                     /locus_tag="P5313_05230"
     CDS             complement(2638..3174)
                     /locus_tag="P5313_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79800.1"
                     /translation="MKNFVFEEVSGFYKIIALEPFRRTEGVAFDIVPMDALPRINGID
                     RVIHAKDALSPGSVGGVERPWYVHPHQEDNLIVLHGKRIVDIYSRAVGRMETFTVEPQ
                     RILKDGKVIFDGPAMLVWPVNVYHRIVSGPDGSASLNFAVRSKGFDVRTNFSIYDLDI
                     KTGEHRVIREGYKDQMTG"
     gene            complement(3213..4106)
                     /locus_tag="P5313_05235"
     CDS             complement(3213..4106)
                     /locus_tag="P5313_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79801.1"
                     /translation="MLFIDRVDGRRIRDPGPFHAILPFLMRGRNESAVYYSKDIDVDS
                     AVRYVHRRNAESGERTFTLFGLILTAAAQTFVLKPRLNRFVHGRSLYERKDIKFSFIV
                     KKRLTEDAKETTAQVTFPPDVTVDSAADIISRAVELARGQDLTPDEREASIIHRIPFG
                     KFVVSRLFRLLDRFNVAPRFMLASDPLYSSAFFANLGSIGLDTPFHHLYEWGTASLFI
                     VVGRLFQQESRGPDGAYRKKNYVNVKMTLDERIADGLYFARAAALFQRLVQHPERMER
                     PPDARESDPEGKPSGPDAVYL"
     gene            complement(4109..4384)
                     /locus_tag="P5313_05240"
     CDS             complement(4109..4384)
                     /locus_tag="P5313_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRY79802.1"
                     /translation="MARKESTVTFKADEELLGALEGVPNRSEFIRHAVLAALDNRCPL
                     CRGTGILTPEQKVHWETFSRRHRIEECVDCHAWHLVCEADPAAPAGP"
     gene            complement(4396..5130)
                     /locus_tag="P5313_05245"
     CDS             complement(4396..5130)
                     /locus_tag="P5313_05245"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HRY79803.1"
                     /translation="MSGTAKSDSGRQILGILGGMGPEATADFFRKVVDATPAAKDQDH
                     IHILVNCDPSIPDRTENILGRGPDPLPALIEAGKRLAAAGATVGAIPCMTAHHWMDAL
                     RAALPFPFLSAFEELGRRIRSDYPQAKRIGVLATTGSIRAGLYEKHLTDWRIHFPDEE
                     AQTRFVMEAVYGKHGIKAGNRGDEPRRLLREAGNRLVARGADLLVAGCTEIPLALKPG
                     DFEVPVLDPMQVLARAAVDYCRGGRA"
     gene            complement(5220..6665)
                     /locus_tag="P5313_05250"
     CDS             complement(5220..6665)
                     /locus_tag="P5313_05250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRY79804.1"
                     /translation="MEEMRRTGLGDKARLLAALTVLLLAGFLGTGYFAFLAARDSIRA
                     NIEKNDLPVAAESVYARLQKDLVEPVFVSSMMANDTFLHDWIVGGERDQGAVVRYLGE
                     IKARYGAFTAFFVSERTGRYYYPEGVLKIVSPSEPRDEWYYRVRSMSEPYELNVDPDL
                     ANADALTIFINYRVIDTDGTYLGAAGVGLAVDSMTRMVATLKSEYGTNVYFVDKAGRI
                     VAGEPYGGPGTEDPALAPIYRRDLRVGPGSYRYRRGSENVLLFVRWLPEVGWFLFVER
                     PEDAAIAGARRALYLNLVVYILILVLALLAAGFTVNRFQSRLERTASYDPLTGAMNRL
                     AFQAVADHAIRETRREGRPLSVILFDIDHFKAVNDTLGHAAGDEALKTVVRTCAATLR
                     ASDLLCRWGGEEFLVLLPASGPDGAFAAAEKLRAAVSAERLAPGSPTVTISAGTATLR
                     PEEDLSSLVQRADERLLEAKRTGRDRTVRAG"
     gene            complement(6732..8021)
                     /locus_tag="P5313_05255"
     CDS             complement(6732..8021)
                     /locus_tag="P5313_05255"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRY79805.1"
                     /translation="MKPVEIAILGFGTVGSGAYRILNEHAKAIARRAACPIAVKRILV
                     RDASRERAVPTPPGVLTESFEEILADPDIRIVVEALGGEEPALGYTLAALEAGKDVVT
                     ANKLALALHGRRIFETARAAGRRIAFEGAVAGGIPVIRALREGLTANNIERITGILNG
                     TTNFILSAMTDRGMEYQDALGEAQSLGYAEADPSADVEGFDAAYKLAILASLGFEGYV
                     DPRRIPRKGISTVTAQDIALADKFGYRIKLLAGAERADGSLHLSVTPSLVPKGHPLAN
                     VNGAYNAVTIEGDSVGQLMLYGKGAGDLPTGSAVVSDIIELVRFPDLSESEIIWQDRE
                     MELSDSTARPSEYYVRLLVKDLPGVLGQIATMLGVHGISIATVTQELDNAGRATLLIF
                     THECERSSVEAAAGTLRTMPFVLSIANILPLWAGTRG"
     gene            8177..8467
                     /locus_tag="P5313_05260"
     CDS             8177..8467
                     /locus_tag="P5313_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HRY79806.1"
                     /translation="MPEDQPNNPLHGLTLEAILRLLVEKYGWEGLAERIPVRCFSVDP
                     SVGSSLAFLRRTPWARAKVEALYLGRLRPRRKGRGRKPGGGNRGRPVEPREP"
     gene            8483..8728
                     /locus_tag="P5313_05265"
     CDS             8483..8728
                     /locus_tag="P5313_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79807.1"
                     /translation="MEITLVIVGGVIVITLIGVVGDLLGKSVKSRSGADPKALRDLSR
                     RVEDLERLSLDREERIRRLEADVAFANKLLEDRSGKA"
     gene            8828..9259
                     /locus_tag="P5313_05270"
     CDS             8828..9259
                     /locus_tag="P5313_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRY79808.1"
                     /translation="MADERITVRTRIRAPVARVWDAYTTPEDITKWNFASDDWHCPSA
                     HVDLRVGGTFSSRMESKDGKAGFDFAGRYLDVRRHSRIEYEMGDGRGVTVEFRPRTDS
                     TDVEVAFDSDGVYSRERQAEGWGAILENFRKHVEGSGEAKS"
     gene            9341..10633
                     /locus_tag="P5313_05275"
     CDS             9341..10633
                     /locus_tag="P5313_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79809.1"
                     /translation="MLETESPAPAPIPGGWKRNVAVFLTSQALSLFGSALVQYALMWH
                     ITLTTKSGTMMALMIILGFLPTFFLSPFAGVFADRHDRKRIIMIADAGIALATLGLAL
                     VYARGTEAVGLLLAVSALRSVGQAFHMPAVGAILPQMVPRDSLMRVNALNGTVQSGLS
                     FAAPVAAGALLSLAPFQVILFVDVATASLAVGLLALFLHVPPHAKASHAQATGYFEDL
                     KLGFRYVRDHRYLVGLFVYMALLFFLIAPAAFLTPLQTARTYGPEVWRLTALEIAFSA
                     GMTVGGALLAALSGFRNRMGTVVASTLVMALCTAALGLAPPFWVYIGFMVLFGLAIPY
                     FNTPLTTLLQEHVEPDYLGRVYSINSMISSSMMPVGMLAFGPLGDLVRIEILLIATGA
                     AIAALGIGVRMSRTLMEMGKPVPSSKVPMETAPDPAGA"
     gene            10680..10835
                     /locus_tag="P5313_05280"
                     /pseudo
     CDS             10680..10835
                     /locus_tag="P5313_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
     gene            10908..11282
                     /locus_tag="P5313_05285"
     CDS             10908..11282
                     /locus_tag="P5313_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRY79810.1"
                     /translation="MSPGSDPGTAAVDAFIAGYPPETRKLLSEIRALIRENAPGAAEK
                     IAYGIPTFTLNGNLVHFSGYERHIGFYPGSEGIAAFEAELAGYKRAKGSVQFPIDRPL
                     PRDLIARIVRFRVEQNGGRKKR"
     gene            11294..12475
                     /locus_tag="P5313_05290"
     CDS             11294..12475
                     /locus_tag="P5313_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY79811.1"
                     /translation="MSGVRSGARRLLVLALVSVFPARIPARPPAIPPGIDLAIRNCIL
                     IDGTGSPPVRDAVLLIGEGRVLAAGPAAAVGIPPGSRVLDLDGAAVLPGFVNAHVHYA
                     LDEERLARWLESGVTSVRDMSSFGMPPAEVARWRARTRERPDLARLYAVGPMITVPGG
                     YGSSFVSTPAEARAAVDRIADAGLDAVKASLEDGYAGVSGLPQFDDATLDALVAAAKA
                     RGLRVTMHVTEARFLRRAVLAGVDELAHIAWDRVDPDTWRRAVSAGILLIPTFSVFRS
                     YGAPVQSCVANLRAFRLAGGRVALGNDYGGGPGEFEGGIPMFEIECMLAAGMTPMEVI
                     TACTRTSAEACAADREIGTLEPGKLADLIVVRGDPLSDPGVLRDPVLVMKEGRIAADR
                     R"
     gene            12506..14104
                     /locus_tag="P5313_05295"
     CDS             12506..14104
                     /locus_tag="P5313_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HRY79812.1"
                     /translation="MTDLDLILGIDSSTQSTSGIVIDRRDFSIAARARVGYRDDPRLA
                     SYGLTGPSPVLPPREPGEADQPPGLFLDALECLLADLGPAVLGRVAAVNVSAQQHGQA
                     WLGAGGETAVSDLRGRGCGAEGERGLAARIGPGFSYDRAPIWMTSSTAEEAEELRSAL
                     GGRDAMTARSGSDSPLRFSGAVLRRTARRFPEAYARTTVCHLLSSFLSAILSGRPRAP
                     VDWGNGAGTSLMDWSGRRWDPDLLEAASRGLPDGAAGLAGRLPLLSHPLAAAGGIAAY
                     FSERFGFDPACVVLAGSGDNPQSKVLAPGALLSLGTSFVFMTDGTKPHPAANAMYDGL
                     GTPFLFGCRTNGALCWESVRTFHGLGANDFRASERALAVTAPGSVLRVLQGERESFPE
                     SRETDYGRPAGFEADYAGVVDSSLGLIYLASRPFAADVAAVAVTGGAAASEGTLRRIA
                     AIWNRPVAIIGEAGAAAGAAVAAAAALEPESGRDGYVARAAGAVSRRGGAVDPVPADV
                     RAYHGESGYLSRLEDLLERGALGI"
     gene            complement(14165..14893)
                     /locus_tag="P5313_05300"
     CDS             complement(14165..14893)
                     /locus_tag="P5313_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRY79813.1"
                     /translation="MPPKPKSPELSTLASPIKKRSVVHFVIDKLKEAMILKELKPGDY
                     LPSESELTRQLGVGKTSVREALKQLEAMGIVEIRQGHGTFIPQKPAADAINPLIFQMI
                     LEQATNEDLLELRMMFEPAYALIAMDKARPDQIRALEDSVDQFERKIRAGIHTAEDDL
                     AFHERMLECTGNPYIVRIGTTIHQLFKTSVSRSMRAIPDTALRDHKAILAAFLSRDPR
                     KLVEAIRNSHKGWAYSLQVTAGNS"
     gene            15075..15590
                     /locus_tag="P5313_05305"
     CDS             15075..15590
                     /locus_tag="P5313_05305"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY79814.1"
                     /translation="MRIRSLIDRLSDALNGAACFLVALGTAAFALLLFASVVARYVFR
                     HPLIFSAEVAKLLFIWSTFLATSVAYKEKIHIRFEFINRRLGDRGVKGTEIAVHAAAL
                     VFFAVIIGKLWEFTSAIWETYFPIIGLSQGWLYVSVLVGFSLMLVHALAHLLEAVEAY
                     GDSVAGAGREA"
     gene            15587..16867
                     /locus_tag="P5313_05310"
     CDS             15587..16867
                     /locus_tag="P5313_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY79815.1"
                     /translation="MIWAALACFVVLVFLRFHIALAIGISSMIAIVFFSDLSLLVLAQ
                     RLVVGVDSYVFLAIPLFILAGNLMNAGGITDRIFAFARALIGHFRGGLGHANVLASMI
                     FAGMSGSAVADAGGLGAIEMKAMDAQGYPREFSASITVASSVIGPVIPPSIPMVIYGA
                     MAEVSVGRLFLGGILPGILVAVSLMVLVYIFSVRHDFPRDPKATFAVFRTRFLESFFP
                     ILTPAVILGGIVFGIFTPTEAAAVAVVYAFILSFFVYRTVRLRDLPGILIDSMKTTAI
                     TTFVMATTSTFSYILLLGQFSDWVVGFLTSVTMNKFVMLFILNLILLFFGCIMEAGVA
                     LILLVPILVPVLNAVGIDLVHFGVVMVVNLMIGVATPPVGMCLFVVSHVANIKLERLM
                     RAILPFLVPPILVLFLITYVPPLVTWLPNLVFGK"
     gene            16909..17895
                     /locus_tag="P5313_05315"
     CDS             16909..17895
                     /locus_tag="P5313_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY79816.1"
                     /translation="MKRFPVLFAVASLVLALPLQAQAKLVLKLGHGHTATHSYQLGME
                     QAKKALEEKTGGRISIQFFPSNQLGDERQMQEALSVGTQDMTITGLANLYDPAFALFD
                     FPFQYANRAQIKAVMYSPLMDRMNEALIRKGLRIVGLMEVGFRNVTSKKPIRSPADLK
                     GMKIRTPESPAQVETFKALGALPTPMPFGELYGALQQGVVDGQENPLENIYNGKLYEV
                     QKYVIETRHIYNFAYVLVSEKTWQKLSAADQAALKEAVKFGSFWQIDWAEANEAKYAQ
                     MLKEKGMEFIQVDREAFRKGAEGAYSSKFVKDLEPRASAILAEIRSIIATVK"
     gene            17967..18746
                     /locus_tag="P5313_05320"
     CDS             17967..18746
                     /locus_tag="P5313_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY79817.1"
                     /translation="MTIIDADCHISPTPESGNSIGREELLARMDRAGVAKALVWLQPP
                     YRRDGIEASNRYVYESASLHPDRFLGFGWADPSLGVEHAVREARRCLSDYGFRGVKLN
                     GAQNGFPIDDPERALPVIDAIAEAGGVLAFHIGADSYNNTHPWRLAKVSDRHPRLPIL
                     AVHMGGASFDDMSDLVIEAAASRPSITLIGSAVRSIPILKAVRKLGPDRVCFGSDTPF
                     ELMHVEVAKYLTLLEGEIPPEDIERVMGGNLARILGVKAGA"
     gene            18743..19168
                     /locus_tag="P5313_05325"
     CDS             18743..19168
                     /locus_tag="P5313_05325"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY79818.1"
                     /translation="MSRPLHPLLPNSEAVRSGDWIFFGGIVAKDGRGNPAFPYDVTAQ
                     TAAVVGRLESYLRAAGLSLGHLVRVEVYLPDIRYYDEMNEAYARVMPRPLPPRKVLVS
                     PLTVEGALVEFTAIASRAVPVVLAPPGEPASGTGREDPP"
     gene            19213..20625
                     /locus_tag="P5313_05330"
     CDS             19213..20625
                     /locus_tag="P5313_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79819.1"
                     /translation="MPEPRRSTALEPSSPSRLLSRGKAYLASFPRPVWYLIAATFVES
                     CGRFMVIPYLSLYMRDVGVGLGTLGLVLGAAPVANVVFGAWGGQLSDRWGRKPVQILG
                     VAASGLSMFGFAFAGPRPVLLAVLNFMNGMTRTFYRPATGAALSDHCPPERRSEAFAL
                     NRIALNAAFGLGPVAGVALFQASPRTGFILGGLLNLAVGLYIALVVPESAPGRIGPAA
                     RDVRSDEPADARVAQGAAGDRAGHSGDGTSGRNAWAEIVRDAGFWVWTAGMTLVWGAY
                     DLIQSFLPLHFRENGTDLWIYGAILSMNAFICVTVQLPLSRRLRVSPIAPAAAWSKAA
                     FAAGFLGFAFLRSPALLLASMLILSLGEVWGSAVQVRYIPEHARPALLGRYMGLSMVS
                     ELGRGIAAPAAGFLMGAAGGEAVFLMAAAFSVGGGALLHVAGKAQDDAARFPGRPRPN
                     GSRRAGGSRTRTRRKTPRGV"
     gene            20655..22010
                     /locus_tag="P5313_05335"
     CDS             20655..22010
                     /locus_tag="P5313_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY79820.1"
                     /translation="MSEESKSPGLNILVVDDEPNIRKMLSFCLAGEGHSVTSVGDPGE
                     AVEEARRRPYDLVFLDLRLGERSGMDLIVPLASDSPWMKIVVITAYASVESVVQAMRL
                     GAEDYLPKPFDPEQVRILARRIGRLRAMEAEVAALREYAGSRGAEGSFESRDPAMLRV
                     VETARRAAESDAIVLLCGESGTGKSLLARAIHGWSPRSAKPFMTVSCPAVPQELLESE
                     LFGHVKGSFTGALRDHPGRIAACEGGTLFLDEVADMAPPVQAKFLRFIQDKEYERLGE
                     SRARKADVRIIAATNADLEERVAQRGFREDLFYRLAVIRLTLPPLRERPDDIEPLASG
                     FLTEFSRAYHRTFLGFTEEARAALRRRAWPGNIRELRNSIERAAILARGPQIDIRDLG
                     GDFPSGPGSPAVGDRVPLSEIERLHIRRVLASAGSLREAASVLGIDPATLWRKRKLYG
                     I"
     gene            22171..>22793
                     /locus_tag="P5313_05340"
     CDS             22171..>22793
                     /locus_tag="P5313_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRY79821.1"
                     /translation="MTLRRKILAGYGLACLLLALVVAWAIANLVALGKATASILRENY
                     RSILAADGMAGALDRLDSGALLVLAGDPDGGQARFREGEALFFSWLARAEDNITIEGE
                     AELVRAIRSDFEAYRTALLASVEGGRKTEIRMWTAYRETLRPLYRKTREGCGRLRVLN
                     ENVMYAARDAAGLRARKAIWSTALVAASALVSALVFSLVFADRIARPL"
BASE COUNT         3454 a         7719 c         7728 g         3892 t
ORIGIN      
        1 gcccatcgcc ctggaactgt aggagaatag gataatggtc aggaatctgg gtatcggaac
       61 cggacgccgc aagacctccg tcgcccgcgt ctatctccgg gaaggcaccg ggaagatcct
      121 cgtgaacggc ctggaggcgg acaagtactt ccgcttcgag gaactgttca acaaggtccg
      181 cggtcccctg gttgtcaccg ccaacgagaa caagctggac gtcgtcatca acgtcgtcgg
      241 cggaggcatg aacggccagg ccggggcctg ccggcacggc ctcgcccgcg ccctggccca
      301 gttcgacccc gcgaaccaca cggccctgcg cacgaacggc atgctctccc gggatccccg
      361 aatggtggag cggaagaagt acggccagcg cggagcgcgc cgccggttcc agttctccaa
      421 gcgctgatcc gggaccgacg cgatgcgcgt cgaatccctg gaaatcggag cctccggcat
      481 cgtgcgactg cgcacggacg ggggctcctg ttttttgttc cgcctggaac aactatcgcc
      541 ttccgcgggc cccgttccct cgatcgggca ggaagccggg gacacgttcc tggccggcgc
      601 gaaggaagcc tcggggatct acgcggccga aaccaaggcc ctggagctcc tggcccgggc
      661 cgagcagacc cggtacctcc tgaccctgaa actccgcaag cgggggatgg gcgacggatc
      721 gatatccccg gccctggacc ggctcgaggg gcaggggctc ctgtccgacg agcgcttcgc
      781 ccgcgcctgg gccgaggaga gggcccgccg ccgcggggag gggccggccc ggatcgcggc
      841 gggcctggcc gcccgagggg tttccgagga cctggcccgg cgtgtctgcg ttgaactata
      901 cccggaggag gcccggggag aggccctgct gagggcggcg cgcagactgc tccggtccgg
      961 ccggaaggat ccggaggccg cggcacggaa gctccgctcc gagggttgga aaccctcgga
     1021 aatccgaggt gccctggaag cgatacgcaa ggaaggggac taaatgcgcg tcttgacaat
     1081 ctttcgcatt ggattcaata tagaagaaac ttaagccaac cttgggagct ttccgcatgg
     1141 atgacgatat cctgacgatc gaagaagtcg cacgctacct tcgggtatcc gagcggaccg
     1201 tctacgactg ggcccagaag ggggagattc cctccggcaa gatcgggacc gtctggcgct
     1261 tcaagaagag cgagatcgag cgctgggtca acgaacgcct ctcctccaac cggagttccg
     1321 cggcctccaa tcccgtcctg attcggaacg tcctcacccc cgagcggatc atcttcccga
     1381 accaccccca caagcgggac gccctcgtgg ccctggcgga aaccctggcc acggcgcccc
     1441 aggtaaaaag ccgcccggaa ctggtctcgg agatcctccg ccgggaggag ctcatgagca
     1501 cggccatcgg ccggggaatc gccatccccc atgtccggct gtcctccgtg acggacctcg
     1561 tggtggccgt ggggatcagc aagtgcgaca tcgtggattt cgcgtccttc gacgactccc
     1621 cggtccggat cctcttcatg atcgcctcgg cgtacaacca gcacgcctac tacctgcaga
     1681 ccctttccta cttcagctcc aagctcaaga acgcggagct gcgggaaacc ctgctcaagg
     1741 ccacggaacc ccaggcggtc tacgacatct tcgccaagtc cgacgaagcc tgagccccgc
     1801 cggacttgcc ggagcccggg gcctatgggt atatttcgat cggaaaaatc atgcccggtt
     1861 cagccgatcc ggagcgccgg gcgctcggga tcggcccggc ggcaagaggt ttgatatgcc
     1921 cacctacgaa tacgagtgtt cctcctgcgg ccacaccttc gaagccttcc agagcatgtc
     1981 cgacgccccc ctttcctcct gccccgagtg cggcaaggcc gtcaagcggc tgatcttcgg
     2041 gggcaccggg gtcatcttca agggctccgg gttctacgtg aacgactccc gcaagtcctc
     2101 ctccggaagc ccgaaggccg gagccccggc ggccaaggac ggtgccgtga aggacgtccc
     2161 ggcgaaagag ggagccgcca aggacagccc cgcccccgcc aaggcgcagg agagcgcgtg
     2221 agcccgtccg cggccgtccg ccgggaggga cgggggtgaa acgcccccga tacttctgcg
     2281 agcactgcgg ggcggaggtc ggccgggacg cctcggtctg cccgaaatgc ggaaggttct
     2341 tctcctccgt gaaatgtccc cgttgcggat tttccgggga acccttcctg ttccggaacg
     2401 gatgtcccac ctgcggttat tccgcggctt ccggaagccc ggccgccccg aagtccgggc
     2461 gcccgacggc ctcggggccc ctgcccggct ggatctatgc cgccgccttt atggcgctgg
     2521 cggccgccct ggccgccgtc ctcatcgcgg cgcgctgatc tgatccttgg gaccgtgcgc
     2581 gggcggcatc atccccggcc gccccggaaa cccgatcgag cctttcggaa ggttccgtca
     2641 gcccgtcatc tggtccttgt atccctcccg gatcacccgg tgttccccgg tcttgatgtc
     2701 caggtcgtag atggagaagt tcgtccggac gtcgaaaccc ttggagcgca cggcgaagtt
     2761 cagggaggcg gacccgtcgg gtccggagac gatccggtga tagacgttca cgggccagac
     2821 cagcatggcc ggtccgtcga agataacctt cccgtccttc aggatccgct gaggctccac
     2881 ggtgaaggtc tccatccgcc cgaccgcccg gctgtagata tccacgatcc gcttgccgtg
     2941 caggacgatc aggttgtcct cctggtgggg gtgcacgtac cagggccgtt ccacgccccc
     3001 cacggaaccc ggggacaggg cgtccttggc gtggatgacc cggtcgatgc cgttgatccg
     3061 gggaagggca tccatgggga cgatatcgaa ggccaccccc tcggtcctgc ggaagggttc
     3121 cagggcgatt attttgtaga atccggagac ttcctcgaag acaaagtttt tcataccgcc
     3181 tccttctttc cttaaggatc gcgacttcgg gtctataagt aaacagcgtc ggggccggaa
     3241 ggtttcccct ccgggtccga ctcccgggca tccggaggac gttccatccg ttcggggtgc
     3301 tggaccaggc gctggaagag ggccgcggcc cgggcgaaat acaggccgtc cgcgatgcgc
     3361 tcgtccaggg tcatcttcac gttgacgtag ttcttcttgc ggtaggcccc gtcgggcccc
     3421 cggctttctt gctggaagag cctgcccacc acgatgaaga ggctcgcggt accccattcg
     3481 tacaggtgat ggaagggggt gtccaggccg atgcttccca ggttggcgaa aaaggcggag
     3541 gaatacaggg ggtccgacgc cagcatgaag cggggcgcca cgttgaaacg gtccaggagg
     3601 cggaagagcc gggagacgac gaacttcccg aagggtatgc ggtggatgat cgaggcctcc
     3661 cgctcgtccg gggtcaggtc ctgcccccgg gcgagttcca cggcccggga gatgatgtcc
     3721 gcggcggaat ccacggtgac gtccgggggg aaggtcacct gggcggtggt ctccttcgcg
     3781 tcctcggtca gacgtttctt gacgatgaag gagaacttga tgtccttcct ctcgtagagg
     3841 gagcggccgt ggacgaagcg gttcagccgg ggcttcagca cgaaggtctg ggccgcggcg
     3901 gtcaggatca gcccgaagag ggtgaacgtg cgctccccgg attccgcgtt ccgcctgtgc
     3961 acgtaccgta ccgccgagtc cacgtcgatg tccttggaat agtagaccgc ggactcgttg
     4021 cggccccgca tcaggaaggg gaggatcgcg tggaaggggc cgggatcccg gatccgccgt
     4081 ccgtcgaccc ggtcgatgaa cagcatggtc agggtccggc cggggccgcg ggatccgcct
     4141 cgcagaccag gtgccaggcg tggcagtcga cgcattcctc gatgcggtgc cttcgggaga
     4201 aggtctccca gtggaccttc tgctccgggg tcaggatgcc cgtgccgcgg cagaggggac
     4261 agcggttgtc cagggcggcc aggaccgcgt ggcggatgaa ctccgagcgg ttcggcacgc
     4321 cttccagggc gcccaggagc tcctcgtccg ccttgaaggt cacggtcgat tccttgcgcg
     4381 ccatggttcc gccgcctagg cccgtccgcc ccggcagtag tccacggccg cccgggccag
     4441 tacctgcatg ggatccagga ccggcacctc gaagtccccg ggcttcaggg ccagggggat
     4501 ctccgtgcaa ccggccacga ggaggtccgc cccccgggcg accaggcggt ttccggcttc
     4561 ccggaggagc cggcggggtt cgtcgccccg gttccccgcc ttgatcccgt gcttgccgta
     4621 gacggcctcc atgacgaagc gggtctgggc ctcctcgtcc gggaagtgga tacgccagtc
     4681 cgtcaggtgc ttctcgtaga gcccggcccg gatgctgccc gtggtggcca ggacgccgat
     4741 gcgcttggcc tgggggtaat cggagcggat ccggcgtccg agttcctcga aggcggacag
     4801 gaaggggaag gggagggcgg cccgcagggc gtccatccag tggtgggcgg tcatgcaggg
     4861 tatcgcgccc accgtcgccc ccgcggccgc gaggcgcttg cccgcttcga tcagggcggg
     4921 caggggatcc ggaccccgtc ccaggatgtt ctcggtccgg tccgggatgg agggatcgca
     4981 gttcacgagg atgtggatgt ggtcctgatc cttggccgcc ggggtggcgt ccaccacctt
     5041 ccggaagaaa tcggccgtcg cctcggggcc catgccgcct aggatgccca ggatctgcct
     5101 gccggaatcg gacttcgccg tgccgctcac gaaaacctcc tatatctcgg gaatcgtcag
     5161 tatacgccgg gttgcggcgg ggcgcaacgg aaatcccgca gatcgcggta aggaaccttt
     5221 catcccgccc ggacggtccg gtcccggccg gtcctcttgg cctccaggag ccgctcgtcc
     5281 gccctctgga ccagggacga aaggtcctcc tccggccgga gggtcgccgt gccggcgctg
     5341 atcgtcaccg tcgggctccc cggggccagc ctctcggccg atacggccgc ccggagtttt
     5401 tccgccgccg cgaaggcccc gtcgggcccg ctcgcgggca ggagcaccag gaattcctcc
     5461 ccgccccagc ggcacaggag gtccgaggcc cggagggtgg ccgcgcaggt ccggaccacg
     5521 gtcttcaagg cctcgtcccc ggcggcgtgg ccgagggtgt cgttcacggc cttgaagtgg
     5581 tcgatgtcga agagaatgac cgagagaggc cggccttccc gacgggtttc ccggatggcg
     5641 tggtcggcca cggcctggaa ggccaggcgg ttcatggccc ccgtcagggg gtcgtaggag
     5701 gcggtccgtt ccagacggct ctggaagcgg ttgacggtga atcccgcggc caggagggct
     5761 aggaccagga tgaggatgta gacgaccagg ttgaggtaga gggccctgcg agccccggcg
     5821 atggccgcgt cctcggggcg ctccacgaag aggaaccagc ccacctccgg aagccaccgg
     5881 acgaagagca ggacgttctc cgagccgcgg cggtaccggt aggagcccgg tccgacacgg
     5941 agatcccggc ggtagatcgg cgccagggcg gggtcctccg tccccggacc cccgtagggt
     6001 tcgccggcga cgatgcggcc cgccttgtcc acgaagtaca cgttggtgcc gtattcgctc
     6061 ttcagggtgg ccaccatccg ggtcatggaa tccacggcca ggccgacccc cgcggctccc
     6121 aggtaggtcc cgtccgtgtc gatcacccgg taattgatga agatggtgag cgcgtccgcg
     6181 ttggccaggt cggggtccac gttgagctcg tagggttccg acatggaccg gacccgatag
     6241 taccactcgt cccggggctc ggagggagac acgatcttga ggactccctc gggatagtaa
     6301 taccggcccg tgcgctcgga cacgaaaaag gccgtgaagg ccccgtaccg ggccttgatc
     6361 tcccccaagt accggaccac ggcgccctgg tcccgttccc ctccgacgat ccagtcgtgc
     6421 aggaaggtgt cgttggccat catggaggag acgaacacgg gctccaccag gtccttctgg
     6481 agccgggcgt acacggactc cgccgccacg gggaggtcgt tcttctcgat gttggcgcgg
     6541 atggagtccc gggcggccag gaacgcgaag tagccggttc ccaggaaacc cgccagcagg
     6601 agcaccgtca gggccgcaag cagcctcgcc ttgtcgccca gccccgtccg tctcatctct
     6661 tccatgaccg aaaggatcgc atatttccgg gaaatcgcaa ccgtaaaaac gaggtatccg
     6721 gcgaaccggg atcagccccg ggtgcccgcc cacaggggca ggatgttcgc gatggacagg
     6781 acgaagggca tggtccggag ggtgcccgcc gcggcctcca cggaggaccg ctcgcactcg
     6841 tgggtgaaga tcagcagggt cgcgcggccc gcgttgtcca gctcctgggt gaccgtggcg
     6901 atgctgatcc cgtggacgcc cagcatggtg gcgatctggc ccaggacgcc ggggaggtcc
     6961 ttgaccagga gacggacgta gtactccgag ggccgggccg tggagtccga gagctccatc
     7021 tcccggtcct gccagatgat ctcggattcc gagaggtccg ggaaccggac cagttcgatg
     7081 atgtccgaca cgacggcgct gccggtcggc aggtccccgg cccccttgcc gtagagcata
     7141 agctgaccca ccgagtcccc ctcgatggta acggcgttgt aggccccgtt gacgttggcc
     7201 agggggtggc ccttggggac caggctgggg gtcacggaca ggtgaaggga gccgtccgcg
     7261 cgctccgccc cggccaggag cttgatccgg tacccgaact tgtccgccag ggctatgtcc
     7321 tgggcggtca ccgtggagat gcccttccgg gggatgcgcc gggggtccac gtagccctcg
     7381 aagcccaggg aagccaggat ggccagcttg tacgccgcgt cgaagccctc cacgtccgcg
     7441 gagggatccg cctcggcgta gcccagggac tgggcctccc cgagggcgtc ctggtattcc
     7501 atcccccggt ccgtcatggc ggacaggatg aagttcgtgg tcccgttcag gatgcccgtg
     7561 atgcgctcga tgttgttcgc cgtcaggcct tcccgcaggg cccggatcac cgggatgccc
     7621 cccgccacgg ccccctcgaa ggcgatcctc cggcccgcgg cccgggccgt ctcgaagatc
     7681 cggcggccgt gcagggccag ggccagcttg ttggccgtga ccacgtcctt gcccgcctcc
     7741 agggccgcga gggtgtagcc cagggcgggc tcctcgccgc ccagggcctc gaccacgatc
     7801 cggatgtccg ggtcggccag gatctcctcg aaggattccg tgagcacccc cgggggggtg
     7861 gggacggccc gttcgcggga cgcgtcccgg accaggatcc tcttgacggc gatggggcag
     7921 gcggcccggc gggcgatggc cttcgcgtgc tcgttcagga tgcggtaggc tcccgatccc
     7981 acggtgccga agcccaggat cgcgatctcg acgggtttca tcgggtacct cgtttcgacg
     8041 aatgggccgc ggggcaggga cggaatccct tgccggtcct gtctccgggc gtctgattct
     8101 acggaaagaa gcggccggga tcaaggggag gccgaccggt ctggcgccgc ccggcccggt
     8161 ccgctatact ccccccatgc ccgaggacca gcccaacaac cccctccacg gcctcaccct
     8221 ggaggcgatc ctccgcctcc tggtcgagaa gtacggctgg gagggcctgg cggagcgtat
     8281 ccccgtccgc tgcttctccg tcgacccgag cgtgggctcc agcctggcct tcctgcggcg
     8341 gaccccctgg gcccgggcca aagtcgaggc cctctacctc ggccggctcc ggccccggcg
     8401 gaagggccgg gggcggaaac ctgggggcgg aaaccgaggc cggcccgtcg aaccgcggga
     8461 accttgagga acggagcgtt tcatggaaat cacgttggtg atcgtaggcg gagtaatcgt
     8521 gatcaccctg atcggggtgg tcggggatct cctgggcaag tccgtcaagt cgagatcggg
     8581 cgcggacccg aaggccctgc gggacctgtc ccgccgcgtc gaggacctgg aacgcctttc
     8641 cctggatcgg gaggagcgga tccgcaggtt ggaggccgac gtcgccttcg cgaacaagct
     8701 actggaggac cgctcgggca aggcctaggc ggcctgtgga tcctcacttg gggaggcgga
     8761 tgggctggac caaatagatg acaaaaagta taatataagt acatcctgat cgcgatcgga
     8821 gagccccatg gccgacgaaa gaatcaccgt tcgaacccgg atccgcgccc ccgtggcccg
     8881 ggtctgggac gcctatacga ccccggagga catcacgaag tggaatttcg ccagcgacga
     8941 ctggcactgt ccctccgcgc acgtcgacct gcgggtcggc ggcaccttct caagccgaat
     9001 ggagtccaag gacggcaagg ccggcttcga tttcgcgggt cggtacctgg atgtccggcg
     9061 gcattcgcgg atcgagtacg agatggggga cggccggggg gtgacggtcg agttccggcc
     9121 ccggacggac tccacggacg tcgaggtcgc cttcgactcg gacggagtct actcccggga
     9181 gagacaggcg gaaggttggg gggcgatcct ggagaatttc cggaagcatg tggaaggctc
     9241 cggggaggcg aaatcgtgag gcggggcgcc ccgggctccc gccgcggacg tgcggggggc
     9301 ccttctttcc gagcggcccg gctttcgata tattcttcca atgcttgaaa ccgagtctcc
     9361 tgccccggcg ccgatcccgg gcggctggaa gcgaaacgtc gccgtcttcc tgaccagcca
     9421 ggctctgtcc ctcttcggtt cggcccttgt gcagtacgcc ctgatgtggc acatcaccct
     9481 gaccacgaag tccgggacca tgatggccct gatgatcatc ctgggcttcc ttccgacctt
     9541 cttcctgtcc cccttcgcgg gggtcttcgc ggaccgtcac gaccggaaac ggatcatcat
     9601 gatcgccgat gcggggatcg ccctggccac cctgggtctg gccctggtct acgcgagggg
     9661 gacggaggcg gtcggccttc tcctggcggt ttcggccctc cggtccgtgg gccaggcctt
     9721 ccacatgccc gcggtggggg ccatcctgcc ccagatggtg cctcgggact ccctcatgag
     9781 ggtgaacgcc ctgaacggga ccgtccagtc cgggctttcc ttcgcggccc cggtcgccgc
     9841 cggagccctg ctgagcctgg cccccttcca ggtcatcctc ttcgtggacg tggccaccgc
     9901 ctccctggcg gtggggctcc tggccctgtt cctgcatgtc ccgccccacg ccaaggcctc
     9961 ccatgcccag gccacgggct acttcgagga cctgaagctg ggcttccggt acgtccggga
    10021 ccaccggtac ctcgtcgggc tgttcgtgta catggccctg ctcttcttcc tgatcgcccc
    10081 ggccgccttc ctgacccctc tccagacggc ccggacttac gggcccgagg tctggcgcct
    10141 caccgccctg gagatcgcct tctccgcggg gatgacggtg ggaggggcct tgctggcggc
    10201 gctcagcggg ttccggaacc ggatgggcac cgtggtcgct tcgacccttg tgatggccct
    10261 ctgcaccgcc gccctcggcc tggcccctcc cttctgggtc tacatcgggt tcatggtcct
    10321 cttcgggctc gcgatcccgt atttcaacac ccccctcacg accctgctcc aggagcacgt
    10381 ggagccggac tacctgggac gggtgtacag catcaattcc atgatctctt cgtccatgat
    10441 gcccgtcggg atgctcgcct tcggtcccct gggggatctg gtccggatcg agatcctgtt
    10501 gatcgccacc ggcgccgcca tcgccgctct ggggatcggc gtccggatga gccgtaccct
    10561 gatggagatg ggaaagcccg tgccgtcctc gaaggtgccg atggaaacgg cgcccgaccc
    10621 cgccggggcg tgatccgggc gatgccggac ctccggccgg tcgcgaaaag gaggagccta
    10681 tgacctggga cgacctgctc gacgcgctgt ccgcccccgc ccggcgggcc ctggagtccg
    10741 cgggaatccg ggatctccgg gacctcgccg cccgatccga ggcggaactc ctggccctcc
    10801 acggcctggg tcccaagtcc ctcgggcacc tgcggcgggc tctcgcggat tccgtggcgt
    10861 ccgcgcccgg cgcaccggcg gacggcaccg cgccggcggg caagggcgtg agccccggct
    10921 ccgacccggg gaccgcggcg gtggacgcgt tcatcgcggg gtatcccccg gagacgagga
    10981 agctgctttc agagatacgg gccttgatcc gggagaacgc cccgggcgcg gccgagaaga
    11041 tcgcctacgg catccccacc ttcaccctga acggcaacct ggtccacttc tccggctacg
    11101 agcggcacat cggcttctac cccggatccg aggggatcgc cgccttcgag gccgagctcg
    11161 ccgggtacaa gcgcgccaag ggttccgtcc agttcccgat cgaccggccc ctcccccggg
    11221 acttgatcgc ccggatcgtg cggttccggg tggaacagaa cggggggagg aagaagcgct
    11281 gagggctccg gggatgagcg gcgtccgctc cggagcccgt cggctcctcg tcctggccct
    11341 tgtatcggtc ttcccggcac ggattccagc ccgccccccg gcgatccccc ccgggatcga
    11401 cctggcgatc cggaactgca tcctcatcga cgggaccggc tctcccccgg tccgggacgc
    11461 cgtcctcctg atcggggagg gccgggtgct cgcggcggga ccggcggcgg cggtcgggat
    11521 accgcccgga tcgcgggtcc tcgacctgga cggagcggcc gtcctgcccg ggttcgtcaa
    11581 cgcccacgtc cactacgccc tcgacgagga gcgcctggcc cggtggctcg agtccggcgt
    11641 gaccagcgtc cgggacatgt cgagcttcgg gatgcccccg gccgaggtcg cgcgctggcg
    11701 cgcccggacc cgggagcgtc cggacctggc ccggctgtac gcggtcggac cgatgatcac
    11761 cgttcccggg ggctacggct cgagcttcgt ctccaccccg gccgaggcgc gcgccgccgt
    11821 ggatcgaatc gccgatgcgg ggctggacgc cgtgaaggcc tccctggagg acgggtacgc
    11881 gggggtctcg gggctgccgc agttcgacga cgcgaccctg gacgccctgg tcgcggccgc
    11941 caaggcccgg ggcctccggg tgacgatgca cgtgaccgag gcccggttcc tccgccgcgc
    12001 cgtcctcgcg ggggtggacg agctggccca catcgcctgg gaccgcgtcg atccggacac
    12061 ctggaggcgg gcggtctccg ccgggatcct cctcatcccg accttcagcg tcttccgcag
    12121 ctacggcgcc ccggtccagt cctgcgtggc gaaccttcgg gcgttccggc ttgccggggg
    12181 aagggtcgcc ctggggaacg actacggcgg cggccccggg gagttcgagg gaggcatccc
    12241 gatgttcgag atcgagtgca tgctcgccgc ggggatgacc cccatggagg tcatcacggc
    12301 ctgtacccgg acctcggcgg aggcttgcgc ggcggaccgg gaaatcggta ccctcgagcc
    12361 cggcaagctc gccgacctca tcgtcgtccg cggcgatccc ctctcggacc cgggcgtcct
    12421 tcgggacccg gtcctggtga tgaaggaggg caggatcgct gcggatcggc ggtgaaggtc
    12481 gagaccgact cgacggggga tccgcatgac cgacctggat ctaatcctgg gcatcgattc
    12541 cagcacccag agcacctcgg ggatcgtgat cgaccggagg gatttctcga tcgcggcccg
    12601 ggcccgggtg gggtaccggg acgatccccg cctggcgtcc tacggtttga ccggaccgtc
    12661 gcccgtcctc cccccccggg agcccggcga ggccgaccag ccgcccgggc tcttcctgga
    12721 cgccttggag tgcctcctgg ccgacctggg tcccgccgtc ctgggccggg tcgcggccgt
    12781 gaacgtctcc gcccagcagc acggccaggc ctggctggga gccggggggg agaccgccgt
    12841 ctccgacctg cgcggaagag gatgcggggc cgagggggag cgggggctcg cggcccggat
    12901 cggcccggga ttctcctacg accgggcccc catctggatg acctcgagca ccgccgagga
    12961 ggcggaggag ctccgctccg ccctgggcgg ccgggacgcc atgacagccc ggtccggatc
    13021 ggacagcccc ctgcgcttct cgggcgccgt cctccggcgg acggcccggc gcttcccgga
    13081 agcctacgcc cggacgacgg tgtgccacct cctgtcctcc tttctgtccg cgatcctgtc
    13141 cggccggccc cgggctccgg tggactgggg aaacggggcg ggcacctccc tgatggactg
    13201 gtccggccgg cgctgggatc cggacctcct ggaagcggcc tcgaggggcc ttccggacgg
    13261 ggcggcgggc ctggcggggc ggctccccct cctgtcgcat ccgcttgccg ccgcgggagg
    13321 gatagccgcc tatttctcgg agcgcttcgg gttcgacccg gcctgcgtcg tgctggcggg
    13381 ctccggggac aatccccaga gcaaggtact ggccccgggg gccctgctgt ccctgggcac
    13441 cagcttcgtg ttcatgacgg acgggacgaa accgcacccc gccgccaacg ccatgtacga
    13501 cggcctggga acgcccttcc tcttcggctg tcgcacgaac ggcgcactct gctgggaatc
    13561 ggtccggacc tttcacgggc tgggcgcgaa cgacttccgg gcctccgagc gcgccttggc
    13621 cgtgaccgcg ccgggctcgg tcctgcgcgt cctgcagggc gagagggaat ctttcccgga
    13681 atcccgggag acggattacg gccggcccgc ggggttcgag gcggactacg cgggcgtcgt
    13741 ggactcgtcc ctcgggctca tctacttggc ttcccggccc ttcgcggcgg acgtggccgc
    13801 cgtcgcggtg accgggggag ccgccgccag cgagggcact ctgcgacgga tcgccgcgat
    13861 ctggaaccgg ccggtcgcaa tcatcgggga ggcgggggcg gccgcgggag ccgcggtggc
    13921 cgcggccgcc gccctggagc cggaaagcgg ccgggacggg tacgtggcgc gggccgccgg
    13981 cgcggtgtcc cggcgaggcg gggcggtcga tcccgtcccc gcggacgtgc gggcgtacca
    14041 cggggaatcc ggatacctgt cccggctgga agatctcctg gaacgcggcg ccctcgggat
    14101 ttaatccggt ccggttttcc gcgggatcct gccttcggac cccggggttc ggcggaaggg
    14161 agggctacga attccccgcc gtgacttgca ggctgtaggc ccatcccttg tggctgttcc
    14221 gaatggcttc gacgagcttc cgcggatccc tcgacaggaa cgcggcgagg atggccttgt
    14281 ggtcccgcaa ggccgtatcc ggtatggctc tcatggatcg ggacacggag gtcttgaaga
    14341 gctggtggat cgtggtcccg atgcgcacga tgtagggatt cccggtgcat tccagcatcc
    14401 gctcatggaa ggccaggtca tcctcggcgg tatggattcc cgcccgtatc ttccgctcga
    14461 attggtccac cgaatcctcc agggcccgga tctggtccgg ccgcgccttg tccatggcga
    14521 tgagggcgta ggccggctcg aacatcatcc ggagctccag gagatcctcg ttcgtggcct
    14581 gctccaggat catctggaag atcagagggt tgatcgcgtc ggccgcgggc ttctggggga
    14641 tgaaggtacc gtgtccctgg cggatctcca cgatgcccat ggcttccagc tgtttcaggg
    14701 cttcccggac gctcgtcttg ccgacgccca gctgccgtgt cagctcgctc tcggagggaa
    14761 ggtagtcgcc gggcttgagc tccttcagga tcatcgcttc cttgagcttg tcgatcacga
    14821 aatggacgac cgaccgtttc ttgatcgggg atgccaaggt gctgagttcc ggggacttcg
    14881 gtttcggcgg catccggtct ccttgcgaga tagcttaaga gcagatgact ggtattatgg
    14941 tgctttccct gccgacgtca aggctgcggg ccggattccc ctttattttt ccttgacagg
    15001 ttttcggaag ccgtttataa cacaagacaa cagatgactt atgttatgag tttttcttct
    15061 aggaggcgga gggaatgagg atccggagcc tgatcgaccg attgagcgac gcgctgaacg
    15121 gggcggcctg tttcctggtg gccctcggca cggccgcgtt cgcgctcctc ttgttcgctt
    15181 ccgtcgtggc gcggtacgtc ttccgccatc ccctcatctt ctccgccgag gtggcgaagc
    15241 tgctcttcat ttggagcacc ttcctggcca ccagcgtcgc ctacaaggaa aagatccaca
    15301 tccggttcga gttcatcaac cggcggctcg gggaccgggg cgtgaagggt acggagatcg
    15361 cggtccacgc ggccgctctc gtcttcttcg ccgtgatcat cggcaagctt tgggagttca
    15421 cctcggccat ctgggagacc tacttcccga tcatcggcct ttcccaaggt tggttgtacg
    15481 tgagcgtcct cgtcggattc tccctgatgc tcgtgcacgc cctggcgcac ttgctcgagg
    15541 ccgtcgaggc gtacggggac tccgtcgccg gggcggggag ggaagcatga tctgggcggc
    15601 cctcgcgtgc ttcgtggtcc tggtcttcct ccgcttccac atcgccctgg cgatcgggat
    15661 ctcctccatg atcgccatcg tgttcttctc cgacctgtcc ctcctggtcc ttgcccagcg
    15721 cctggtggtc ggcgtggaca gctacgtatt cctggctatc ccgctcttca tcctggcggg
    15781 gaacctcatg aacgcgggcg ggataaccga ccggatcttc gccttcgccc gggccctgat
    15841 cgggcacttc cgcggcggcc tcgggcacgc caacgtcctg gccagcatga tcttcgcggg
    15901 catgtccgga tccgccgtgg ccgacgcggg cgggctgggg gccatcgaga tgaaggccat
    15961 ggacgcccag ggataccccc gggagttctc cgcctccatc accgtggcct cctcggtgat
    16021 aggaccggtc atccctccga gcatcccgat ggtgatctac ggggccatgg cggaggtctc
    16081 cgtcggcagg ctattcctgg gagggatcct gccgggcatc ctcgtggcgg tctccctcat
    16141 ggtgctggtg tacatcttca gcgtccggca cgattttccc cgggacccga aggccacctt
    16201 cgccgtcttc cggacacgct tcctggagag cttcttcccg atcctgaccc ccgcggtcat
    16261 cctgggcggc atcgtgttcg ggatcttcac ccccaccgag gcggccgccg tcgccgtcgt
    16321 gtatgccttc atcctctcgt tcttcgtgta ccgtacggtc aggctccggg acctcccggg
    16381 catcctgatc gactccatga agaccacggc catcaccacc ttcgtgatgg ccacgaccag
    16441 caccttctcc tatatcctgc tgctgggaca gttctcggac tgggtcgtgg gcttcctgac
    16501 ctcggtcacg atgaacaagt tcgtcatgct gttcatcctg aacctgatcc tcctgttctt
    16561 cgggtgcatc atggaggcgg gggtggccct catcctgctg gtgcccatcc tggtgcccgt
    16621 cctgaacgcg gtgggcatcg acctcgtaca cttcggcgtc gtgatggtgg tcaacctgat
    16681 gatcggggtg gcgacgcccc ccgtggggat gtgcctcttc gtcgtgagcc acgtggcgaa
    16741 catcaaactc gagcggctca tgcgggcgat cctgcccttc ctcgttccgc ccatcctggt
    16801 cctgttcctg atcacctacg tccccccgct cgtgacctgg ctgccgaacc tggtgttcgg
    16861 caaataggcg gccgtacggc acgccaacgt atttcggagg agaccgacat gaagagattc
    16921 ccggttctgt tcgcggtcgc gtcgctcgtc ctggccctgc ctctgcaggc ccaggcgaag
    16981 ctcgtcctga agctgggcca cggccacacg gccacccatt cctaccagct gggcatggag
    17041 caggccaaga aggccctcga ggagaagacg gggggaagga tcagcatcca gttcttcccc
    17101 tccaaccagc tcggcgacga gcggcagatg caggaggccc tgagcgttgg gacccaggac
    17161 atgaccatca ccggcctggc caacctctac gatcccgcct tcgcactgtt cgactttccc
    17221 ttccagtacg cgaaccgggc ccagatcaag gcggtgatgt attcgccctt gatggatcgg
    17281 atgaacgagg ccctgatccg gaaaggcctg cgcatcgtcg gtctcatgga ggtcgggttc
    17341 cgcaacgtca cctccaagaa gccgatccga agccccgcgg acctgaaggg aatgaagatc
    17401 cggacccccg agagccccgc ccaggtcgag accttcaagg ccctcggcgc cctgcccacg
    17461 cccatgccct tcggcgagct ctacggcgcc ctccagcagg gcgtggtgga cggccaggag
    17521 aaccccctgg agaacatcta caacggcaag ctgtacgagg tccagaaata cgtcatcgaa
    17581 acccgccaca tctacaactt cgcctacgtc ctggtctccg agaagacctg gcagaagctc
    17641 tccgccgcgg accaggccgc cctcaaggaa gcggtgaaat tcggttcctt ctggcagatc
    17701 gactgggcgg aggccaacga ggccaagtac gcccagatgc tgaaggagaa ggggatggag
    17761 ttcatccagg tggaccggga ggccttccgc aagggggccg agggcgccta ttcctccaag
    17821 ttcgtgaagg acctggaacc ccgagcctcc gccatcctgg cggagatccg gtccatcatc
    17881 gcgacggtga agtgatattc ctgaacgggg acggggcggc cttcgggccg tccccgcccc
    17941 ggccatattc cgagggagcc gaaaccatga cgatcatcga cgcggactgc cacatctccc
    18001 ccacgcccga gtcggggaac agcataggcc gggaagagct cctggcccgg atggaccggg
    18061 ccggggtcgc gaaggccctg gtctggctgc aaccccccta ccgaagggac ggcatcgagg
    18121 ccagcaaccg ctacgtgtac gagtcggcct ccctgcaccc cgaccggttc ctgggcttcg
    18181 gctgggcgga tccgagcctg ggcgtcgagc acgcggtccg cgaggcccgg cgctgtctct
    18241 cggactacgg attccggggg gtcaagctga acggggccca gaacggattc ccgatcgacg
    18301 atccggaacg ggccctgccc gtgatcgacg cgatcgcgga ggccggcggc gttctggcct
    18361 tccacatcgg cgcggattcc tacaacaaca cccatccctg gcgcctggcc aaggtctcgg
    18421 accggcatcc gcgcctgccc atcctggcgg tgcacatggg cggcgcctcc ttcgacgaca
    18481 tgagcgacct cgtgatcgag gcggcggctt cccggccgtc catcaccctg atcggcagcg
    18541 ccgtgcggtc catcccgatc ctcaaggccg tccggaagct cgggcccgat cgggtctgct
    18601 tcggcagcga cacccccttc gagctcatgc acgtggaggt agccaagtac ctgaccctcc
    18661 tggaaggaga aattcccccg gaggatatcg agcgggtcat gggggggaac ctggcccgga
    18721 tcctgggcgt gaaggcggga gcgtgagccg tcccctgcat cccctcctgc cgaactccga
    18781 ggccgtccgg tcgggggact ggatcttctt cggcggcatc gtggccaagg acggccgggg
    18841 gaacccggcg ttcccgtacg acgtgaccgc ccagaccgcc gccgtagtgg gccggctgga
    18901 gtcgtacctg cgcgccgcgg gcctgagcct cgggcacctc gtacgggtgg aggtctacct
    18961 tccggacatc cgctactacg acgagatgaa cgaggcctac gcccgggtca tgccccggcc
    19021 cctgcctccc cggaaggtcc tcgtctcccc gctgaccgtc gaaggggccc tggtggagtt
    19081 caccgccatc gcctcccggg ctgtccccgt cgtgctggcc cctccgggag aaccggcgtc
    19141 cggaaccgga cgggaggatc ccccgtgaat cggccctgga acaagtccgc ataaaaggcg
    19201 atactcccgg ccatgccgga acctcgacgc tccaccgccc tcgagccttc ttccccgtcc
    19261 cgcctcctgt ctcgtggtaa ggcctacctt gccagctttc cccggcccgt ctggtacctc
    19321 atagcggcca ccttcgtgga gtcctgcggc cgcttcatgg tgatccccta tctcagcctg
    19381 tacatgcggg atgtcggggt gggcctgggc accctgggcc tggtcctggg tgcggccccc
    19441 gtggccaacg tcgtattcgg ggcctggggc ggccagcttt cggaccggtg gggccgcaag
    19501 cccgtccaga tcctgggggt cgcggccagc gggctttcga tgttcggctt cgccttcgcc
    19561 ggtccccgcc cggtgctcct cgcggtgctc aacttcatga acgggatgac ccggaccttc
    19621 taccggccgg cgaccggcgc cgccctctcg gaccactgtc ccccggagag gaggtccgag
    19681 gccttcgccc tgaaccgcat cgccctgaac gccgccttcg gcctggggcc ggtcgcgggg
    19741 gtggccctgt tccaggcctc gccgcggacg ggcttcatcc tgggagggct tttaaacctg
    19801 gcggtgggcc tgtacatcgc cctggtcgtg cccgagtccg ctcccggccg gatcgggccg
    19861 gccgcgcggg atgttcggtc cgacgagcct gccgatgccc gggtcgccca aggcgcggcc
    19921 ggggacagag ccggccattc cggggacgga acttcgggcc gaaacgcctg ggcggagatc
    19981 gtccgggacg cggggttctg ggtctggacc gcgggcatga ccctggtctg gggcgcctac
    20041 gacctcatcc agagcttcct gcccctccac ttccgggaga acggcacgga cctatggatc
    20101 tacggggcga tcctgtccat gaacgccttc atctgcgtga ccgtccagct tcccctctcc
    20161 cgccggctcc gggtatcgcc gatcgccccg gccgcggcct ggtccaaggc ggccttcgcg
    20221 gcgggattcc tgggcttcgc cttcctccgt tccccggcgc ttctcctggc ttccatgctg
    20281 atcctgagcc tgggggaggt ctggggttcc gcggtccagg tccgctacat acccgagcac
    20341 gcccggccgg ccctcctggg gcgctacatg ggcctgtcca tggtcagcga gctcggccga
    20401 gggatcgccg ccccggccgc gggcttcctg atgggagccg cggggggcga ggcggtcttc
    20461 ctcatggctg cggccttctc cgtcggaggg ggagccctgc tgcatgtcgc cgggaaagcc
    20521 caggacgatg cggcgcggtt tcccgggcgg ccgcggccga acggatcccg gagagccggc
    20581 gggagtcgga cccgaactag gcggaaaacc ccgcgcgggg tatgatcgga ggcgcgcttc
    20641 gacggagccg caggatgagt gaagagagca aatcccccgg gctgaacatt ctcgtcgtgg
    20701 acgacgagcc gaatatacgg aagatgctgt ccttctgcct tgccggggaa ggccactccg
    20761 tgacatccgt gggcgatccc ggggaagcgg tcgaagaggc gcggcggaga ccctacgacc
    20821 tggtgtttct ggacctccgt ctcggggaac ggagcgggat ggatctcatc gtccccctcg
    20881 catcggactc cccctggatg aagatcgtgg tgatcacggc ctacgcctcc gtggaaagcg
    20941 tcgtgcaggc catgaggctc ggcgcggagg attacctgcc gaagcccttc gatccggaac
    21001 aggtcaggat cctggcgcgc cggatcggac ggctcagggc gatggaggcc gaggtcgctg
    21061 ccctgcggga gtacgccggg tcccgggggg cggagggatc gttcgagagt cgggatcccg
    21121 cgatgctgcg ggtggtcgag acggcccggc gggccgcgga atccgacgcg atcgtcctgc
    21181 tgtgcggcga gagcgggacc ggcaagtcgc tcctggcccg ggccatccac ggttggagcc
    21241 cgaggtctgc caagccgttc atgaccgtat cctgtcccgc ggttccccag gagctcctgg
    21301 agagcgagct gttcggccat gtcaagggct ccttcacggg tgcccttcgc gaccaccccg
    21361 gccggatcgc ggcctgcgag ggagggacct tgttcctcga cgaggtcgcg gacatggctc
    21421 ctcccgtcca ggcgaagttc ctgcggttca tccaggacaa ggagtatgaa cgcctcggcg
    21481 aatcccgggc ccggaaagcg gatgtccgca tcatcgcggc cacgaacgcg gacctcgagg
    21541 agcgggtcgc gcagagaggt ttccgggagg acctgttcta taggctggcc gtgattcggc
    21601 tgacgcttcc gcccctccgg gagaggccgg acgacatcga gcccctcgcc tcggggttcc
    21661 tgacggagtt ttcgcgggcc taccaccgga cgttcctcgg gttcaccgag gaagcccgcg
    21721 cggcgctgcg aaggcgggcc tggcccggga atatccggga gctccggaat tccatcgaga
    21781 gggctgcgat cctggcccgg ggcccccaga tcgacatccg ggatctcgga ggggacttcc
    21841 cttccggccc cggttcgccg gcggtcgggg acagggttcc gctttcggag atcgagcgac
    21901 tccatatccg ccgggtcctg gcctccgccg gatccctgcg ggaagccgcc tccgttctgg
    21961 ggatcgatcc ggcgaccctc tggagaaagc ggaagctgta cgggatctaa cccgacgtcg
    22021 tgcgttttgc aatactcggt gaaagtgctc ttgcaatttg acttgccttc aaacgcggaa
    22081 agccgtaatt ccttgtaata tcaacacatt ttcttggcat gcttattgct ccgtagtatt
    22141 cgattccgag atatggaagc ggggtaaccc atgaccttga ggcggaaaat tctcgccgga
    22201 tacgggctcg cctgcctgct cctggcgctc gtcgtggcct gggccatcgc gaacctggta
    22261 gccctgggga aggccacggc gtccatcctg agggagaact atcggagcat cctggccgcg
    22321 gacggaatgg ccggtgccct ggatcggctg gactccgggg cgctccttgt ccttgcgggc
    22381 gatcccgacg gcgggcaggc gaggtttcgg gaaggggagg cgcttttctt ctcgtggctc
    22441 gcccgggccg aggacaacat caccatagag ggcgaggcgg aactcgttcg ggcaatccgc
    22501 tccgatttcg aggcgtatcg gacggcgctt ctcgcctcgg tcgagggcgg aaggaagaca
    22561 gaaatccgga tgtggaccgc ctaccgggaa acgctccgcc ccctataccg gaaaacccgg
    22621 gagggatgcg gccgtctgag agtcttgaat gagaatgtca tgtacgcggc ccgcgacgct
    22681 gcgggcctcc gggcgcggaa ggcgatctgg tccaccgcgc tggtcgccgc ttccgccctg
    22741 gtttcggcct tggtcttcag cttggtcttc gcggaccgca tcgcgcgccc cct
//
LOCUS       DAOTEI010000036        22579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1501_length_22579_cov_5.865166, whole genome shotgun sequence.
ACCESSION   DAOTEI010000036 DAOTEI010000000
VERSION     DAOTEI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 22579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22579
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1501_length_22579_cov_5.865166"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="P5313_05345"
     CDS             <1..705
                     /locus_tag="P5313_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79822.1"
                     /translation="FPVTPTFESVVSPGFSLDNGEYFARYSFLGESFDLALMGGWFWN
                     DTPGYTVTDKTFVPGVGITALTVRPEYYRTAAAGYAASGAMGPVILKSEGAFYGLRRY
                     QGNPMIYPDGYAEKYSLQYMAGADASAAGFTFGIQFIQDLILDHDPDLVDPELKTTMT
                     FVATRTFLRDTLTAEVFTYMGLDPLNALVKPKVTWDATDALEFFLGAYLFLGDEGDFG
                     RYDSNDGVYLGAKFSF"
     gene            800..1183
                     /locus_tag="P5313_05350"
     CDS             800..1183
                     /locus_tag="P5313_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRY79823.1"
                     /translation="MESDIRLEPSIEEWREQRGRLNETVMAMAGLEIKRFFSLDGQTY
                     RPGALDAKTKEFLGLVASLVLRCDDCILYHTIRCKEEGATTEEFQEACTVGLFVGGSI
                     VNPRLGRYREDQLPDSLPGRLVPGG"
     gene            complement(1118..2071)
                     /locus_tag="P5313_05355"
     CDS             complement(1118..2071)
                     /locus_tag="P5313_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79824.1"
                     /translation="MRMFTVLPLILGLSFAPAFAVDPPILEAGMDPAVREWAFTRGGG
                     EQRNVRFWMRTRREGFLNAYGNFDYYLRVQVLRPDGSIAWDTQYGFDLQGYADTSFVL
                     PGLFFDLSPNKAAPSFGAWKIRTVLFDNAAKVERASREYVLNFSDGSKAAVPGGSGAG
                     SPFSVPPFTHGSWTLRDWGIGLFRQSPSGPGTYDVETRPVAVRASFSLAEIREGYARG
                     EIFGARLAGPPQSAFLNSGGMPLYVFGYALRNPDGSGTGEAAAGRNNKYVNNPGDTVF
                     PLDPRSPGPYRVTLYIRDRDSPSWDEASWKRIGELVFTVTP"
     gene            complement(2144..2716)
                     /gene="pth"
                     /locus_tag="P5313_05360"
     CDS             complement(2144..2716)
                     /gene="pth"
                     /locus_tag="P5313_05360"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRY79825.1"
                     /translation="MVRLYAFLGNHGRQYRNNRHNVAWQVLERLPAHPSLRWERGYKG
                     RWASLDTPSGRVFLLTPETFMNLSGDSVAELMRFHRVEPGELLVVHDELELPLGTVGL
                     KRAGGLGGHNGLRSIRDRLSTPDFLRFRIGIGRPDHDDVAGYVLSDFSKTERELLETA
                     VYPVSVRILERVLSEGFEALEEECRKVKAI"
     gene            2857..4737
                     /locus_tag="P5313_05365"
     CDS             2857..4737
                     /locus_tag="P5313_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRY79826.1"
                     /translation="MTPTRFVRTLRSERFADLDCAVLEYRSRDGSTDVEPRMTLLEEL
                     PLIELVRLADGPAGGRPTAGPGNPAGDAAFDLRGAFVFANGWQSWSPGWELAPGERME
                     RARFVGRLNVFTDHPGLVPRRGEVLASFLGYLRSGGEYLALASLPEGGPPLSFLFRRR
                     EGTVSLLAYAEGHRFRAGDLAARILVVRARSWFALKDVLREAYAPYDLFSRLAFLGAP
                     GEPLVPGGYESWYNHYADIDEGLILADLRNLAGNGNLIDRLYIRPGKPTVFQVDDGWE
                     RTVGDWRVNEARFPRGMKDLAGRIEEAGFIPGLWLAPFLVTRFAPLYREKPEWLLRDE
                     RGRPVAAGWIPAWGHDFYCLDLSIPEVLDYLTGIFDRAVNEWGYRYLKLDFLYAGMLR
                     GSRRNGDPAWIHYRKALERLTSIRNRRNGKPVAYLGCGAPFEASFEFLPLMRIGADTR
                     EDWDWAQARFLRHPGRPSAWISMKDTIGRALWDGTVFLNDPDVVFCRTRRMRLSETEK
                     ELVALTARIFASQVMFSDDTADFEPATEGAFTERIAELYGRISGREFRPERVPGFRDV
                     YTARSRDGRSAAVLNLSDSDAVVPGSWPADKAVLARFRSEGGRTVFAPRSISLFDGLE
                     EA"
     gene            4734..6389
                     /locus_tag="P5313_05370"
     CDS             4734..6389
                     /locus_tag="P5313_05370"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HRY79827.1"
                     /translation="MKRTVYVIGHKNPDTDSVVSALGYARLKRELGMAEATAARAGMI
                     NPQTEYVLSRFKVEVPAFVPDLVPKAEYYLDDAAVTVPAGTPLWDALALMEEHGRTAL
                     PIVDGQGRYRATLHYSAFARNILKKINPRKKAVIPTSVGRMAATIKAQVVTSFDPETE
                     FKARILVAALETESFKRHLDGEARENCIVIVGDRADVQRYVLESKARVLIVTNGAVLD
                     RSLKEIAERNRVSVLLSPYDTSSTALLVIYSTPVETMGDEGLKPVRLDSPLRNLRSPL
                     AESPSRSVPVTDEEGRVAGMFTEGDLLRDPKVELILVDHNELGQAVEGAENYRILEVI
                     DHHRIGSFSTRFPITFINRVVGSTSTIVAGMYREHRIPLPKPIASILLCGILSDTLVL
                     RSVTTTETDRETAEYLAAIADLDIPALGADILAASSAVSSKPARDLVRLDMKTYTVTD
                     RTFSVSQVEVDSPEDLLARREEVLRELAALRSEGGRYLSALMVTDITELSSYLFLSAD
                     KEFESLVKYPEEEDHVYRLKDILSRKKQLVPVLLELAEKALGA"
     gene            6503..6868
                     /locus_tag="P5313_05375"
     CDS             6503..6868
                     /locus_tag="P5313_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79828.1"
                     /translation="MKLLVFVLNKEELLDRVLEAYMEAGITGATILDSEGMGHFLVYE
                     VPLFADFKDFLKGHKPYNKTILSVVPDESAIPRVVRLIDKVCGGFRNSNTGILFTVPV
                     DFAVGLLEQNPPGPEETPS"
     gene            6865..7323
                     /locus_tag="P5313_05380"
     CDS             6865..7323
                     /locus_tag="P5313_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY79829.1"
                     /translation="MTDLSPFFTPQTVVLDFSARKKGDVLRKVSQMLAGPGTGKADSL
                     EDALQRREILCSTGLGEGVAAPHVLLPGLEKTSLAFLRLRDPVDWRSPDGIPVRLVFA
                     LAGPAEDPAAHLQILSRLTRLLREPDFRETVAAAASGEEVASAFRRTEEE"
     gene            7327..7839
                     /locus_tag="P5313_05385"
     CDS             7327..7839
                     /locus_tag="P5313_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRY79830.1"
                     /translation="MTPRTKWDLVRFVSTFSVLLASWLLFAGPPDAYSIAAGTLSAAV
                     LAALTYRFFLDDHEAGARHLIPNPLLLLLYLPYILFRIYAASFILVYDMIAGRMNPRI
                     VHFRTRLRSDLARVVLCSSVTLTPGTIAVLIDEDHLLVHWFNAESGHSGRARREIAES
                     MERFLGRIWS"
     gene            7839..8096
                     /locus_tag="P5313_05390"
     CDS             7839..8096
                     /locus_tag="P5313_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRY79831.1"
                     /translation="MPWDFVLWFFLGSLVLGLVRLVIGPSTADRLVGLNLVAGQVLAL
                     LVLLAVKEDVALYLDVALAYDIFGFLGILAIVKYFTKRRAS"
     gene            8093..8425
                     /gene="mnhG"
                     /locus_tag="P5313_05395"
     CDS             8093..8425
                     /gene="mnhG"
                     /locus_tag="P5313_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRY79832.1"
                     /translation="MNPAYWMELILIFLGMGFALIGNLGVFLMPDPYTRLQASATCST
                     TSVISVILAAMVSSGFSPTTGKLAIILLYFLVSSPVATTIIARYAWESGIVPWRRPKR
                     RLGKGGSP"
     gene            8422..8655
                     /locus_tag="P5313_05400"
     CDS             8422..8655
                     /locus_tag="P5313_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRY79833.1"
                     /translation="MIEASLVLMLLVAILSLAARDLLATIVLLAVFSLLCAVVFYYLH
                     APDVAITEAAVGAGVSTLILVWAVRRTARGEEG"
     gene            8652..8912
                     /locus_tag="P5313_05405"
     CDS             8652..8912
                     /locus_tag="P5313_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79834.1"
                     /translation="MRTLRRVLELLPFLALAVAVILPLAVMPPAPTALRDYCVERGIA
                     ETGAPNLVSAIYLGWRAYDTLGETIVLLLAVTGALYLLGGRE"
     gene            8909..9325
                     /locus_tag="P5313_05410"
     CDS             8909..9325
                     /locus_tag="P5313_05410"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRY79835.1"
                     /translation="MRKTDALSVITGKLGPLVLVFGAYLIAFGHVSPGGGFQGGVVLA
                     SGVLLLAIGRGTDTVLALLTPRLLARIEASAFALLLVTGLAGMLAGKGFFGNFLTAGG
                     PAGPVGFIIALNVLIGVKVGAGVAYLCLILLKDSDR"
     gene            9322..9663
                     /locus_tag="P5313_05415"
     CDS             9322..9663
                     /locus_tag="P5313_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HRY79836.1"
                     /translation="MIPRLLCGAILLTGLAGLVWNRNLIKKVAALSVMNSAVILLFVL
                     GGSETGIRSPILTGTDLPVVDPLPQALMLTAIVVGVAVTALALVLAVRLHERFGTLDS
                     REIEERIRDEP"
     gene            9653..11344
                     /locus_tag="P5313_05420"
     CDS             9653..11344
                     /locus_tag="P5313_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY79837.1"
                     /translation="MSPERLALWIALLPLLAAALPLLEKAFGRPRTEAGPASDLGALL
                     GLAGPLAVLALLYPAVTDGGIVHLTLGEWSPSIGIRYRLDGPAWFASAFTAVVSLCAG
                     LYARARGGYSPTFWFLFLLCHWSLQSALLAADFFHLFVCLELMALTSYVLIAYKKESA
                     ALFASFNYLMVSTAAIVFYLFGLYLLYGASGTLSIEEAGRVLASARPRERGLAALGLA
                     FLAGGLGVRTAFLPFHGWLPDAHASAPHPVSALLSGAMIKVSFFAQWRILSLAEFPML
                     RESFLWLGAAGACAGVVLALCQSDAKRLLAWHSVSQVGYVLAAFGAGSPAGLLAAGLH
                     AAAHGLFKSLLFLSVGSAADAAGSRNAYRMAGAFRGLPEAAAGFVFGALSIAALPPFI
                     GAVSKDLVAYAAGGGLPGALLLAASVGTAASFSKLGILFFSAPEPGSRTPALSAVELG
                     RLRAALRRGALPLAALCLGTGLAAPALVPAAAGLLAPGYTAAFAFSLSGAAKSLGIAA
                     AGFAAARAVLSPRGRALAHRFERLGTGSETTLTLTLLGLALLAALGGWAGRGIPG"
     gene            11351..13036
                     /locus_tag="P5313_05425"
     CDS             11351..13036
                     /locus_tag="P5313_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HRY79838.1"
                     /translation="MTAPGPEVTAVLVVFLAPLAASLGPLVSKAFLDPGLRGGWAARA
                     IPAVCLVLGLLASPAALIRLHAAVTGGGTVAFALGLPSPLAIRYLLDGPAWLACALIA
                     AIGLCAGLYGLVSGGYSPAFWFFFLVALSALYACVLTADLFNLFVSLELLALCSYVLI
                     AYKRTPASLWASYSYLVTSTVAVVFYLLGLYFLYGYAGTLSIPEAAASIQSLGPVPPR
                     DLRMALGFLTVALGVRSAYAPFHAWLPEAHASAPHPVSALLSGATLAAGYFALGRILS
                     LLDVPALREGLLWAGALTALTGGLFAVAQRDAKRLLAWSSVGQAGLCLAALASGSFAA
                     GALHALAHGAAKALLFLSVGRAADEAGSRDLGILQGRAGLGPSLGILIGAASLAGLPP
                     LAGWASKSLAGYAAGGGAAGMILAAASVLSAAAAFRVLPLLVIFRLSGGPREGSPAPS
                     GPGDIRNLAVVLTGLLPLAVLCVAGGLPGIPAAAASLADSGAGPLGIPAFGWPALAKA
                     LPTLGAAAVIAAAAGTRPARRILDLWERRGTGADAALSLLLAGAAAAALSGLL"
     gene            complement(13033..14220)
                     /gene="gguB"
                     /locus_tag="P5313_05430"
     CDS             complement(13033..14220)
                     /gene="gguB"
                     /locus_tag="P5313_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY79839.1"
                     /translation="MILKDLRSALRSNIREYGMYIALVVIMGIFTIATQGIFISSRNI
                     ANLLNQTGYIAILAIGITLVIVIRHIDLSIGFLSGFLGAVAAIAMTAWGVSTWIAILI
                     VLVLGTLAGLLTGYLVAGVGIPAFVASLAGWLIYRGALLTVTLKTGTIIIPNEDFNAI
                     GNGYIPDILPADTAFLPELHKLTLLLGILAIVWLNVRAFQERASRQKYGLEVLPGDMF
                     ALKQLFLSVVLSLITWVVAGYNGMSWTLAIVIVVALIYNFVTTRTVLGRHIYAVGGNP
                     EAAELSGVSVKKITLTVFGSMGMLCGLSGVLFASRLQSATTTAGTLFELDAIAAAYIG
                     GVSAAGGVGKVTGSIVGAIVYMSLTSGMNLLGTDISLQYIIRGAVLVAAVIFDVQTRK
                     AKS"
     gene            complement(14231..15751)
                     /gene="gguA"
                     /locus_tag="P5313_05435"
     CDS             complement(14231..15751)
                     /gene="gguA"
                     /locus_tag="P5313_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY79840.1"
                     /translation="MSEPILEMRNITKTFPGVRALSDVSFRVARGEIHCLVGENGAGK
                     STLMKVLSGVYPHGSYEGEILLSGSPQRFRGIADSERAGIGIIYQELALVPDMTVYDN
                     ILLGHELRSGVTVDLNGTIKKATEMLAKVRLQLDPSTKVRDLGVGKQQLVEIAKALSR
                     DVKLLILDEPTAALNEDDCDNLLDILRGLRRDGVTCIMISHKLKEVIRIADTVTVLRD
                     GRTVRTLDAGKGEVSEGILIQHMVGREIDDVYPKRTSKKFGDVVLEARNWTVYSHGLG
                     RTVLKDVNFTVRRGEIVGFAGLMGSGRTELARSVFGNPDGYRVEGELLVEGRPVRFGD
                     PRAAIDAGVAYASEDRKRNGLILIQDVKQNITLANLRALAARGVVDGNAETKVAEDYR
                     SALSIKTPSIDQKVLNLSGGNQQKVSVAKWLFVRPKVLILDEPTRGIDVGAKYEIYTL
                     MNKLVAEGMSIVMISSELPEVLGMSDRVYIVSTGRITGELPVEEADQEKIMRLATS"
     gene            complement(15849..16943)
                     /gene="chvE"
                     /locus_tag="P5313_05440"
     CDS             complement(15849..16943)
                     /gene="chvE"
                     /locus_tag="P5313_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY79841.1"
                     /translation="MKRIVAILSAVLFLAGIVVSAVAQAGSLPVGIVLPTKDEPRWLQ
                     DEARFKDALAKAGYNVEILFSQGDSAKEKANVESLITKGIKVLIICPQDSAAAAAAAD
                     AARKAGVKVISYDRLITGTASVDYYVTFDSVAVGEAWGDFLVSQAGTRKGLNLYLYAG
                     AASDNNAFLFFEGAWNKLQPKIADGTFVVRNSTEAVAQSKKAKLSREEMGKIIGQVTT
                     NWDFNTAKSKAEADLTRATGAMKGEVFICAPNDGTARAIADAFAVDRAVTKYWITGQD
                     AEKASVQYIIDGKQSMTVLKDVRVLAADAIAAAVAYLKGGTPAKTKSYNNGRAEIPAK
                     PSATTTVTKANVKAAIVDSGYYPASDFKGL"
     gene            complement(17147..18196)
                     /locus_tag="P5313_05445"
     CDS             complement(17147..18196)
                     /locus_tag="P5313_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY79842.1"
                     /translation="MRNPDRSRRALVLAAALLSATLPLVSCRPAPRRAEPGQAPLIGF
                     SLDSLVVERWKRDAEIFIRSARELGADVLLRVADQDHAVQERQIRDLADAGIHVLVVV
                     PNDADRLSSVISEIRARGIRVLSYDRLVRNANVDLYVSFDNVMVGRLMAESVSRAVPR
                     GGFVIINGARSDNNALMLNAGIRGVLDPLEASGAVRIVGEIWPGTWDSEEVKSALEKI
                     VQHDRGIDAVVAGNDMLAEAAISVLSENRLMGNVKIAGQDAELAACQRIAEGVQFATV
                     YKPIDRLALKAAGYAVMLARGEEIRTDAVINDGKYEVPYVRLEPILVTRDLLDATVIK
                     DGFHSSADVYRKVRR"
     gene            complement(18183..19763)
                     /locus_tag="P5313_05450"
     CDS             complement(18183..19763)
                     /locus_tag="P5313_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79843.1"
                     /translation="MKRVLVVDDERPVVETIELMVRRDLSAEFSVVGNASSGRDAVEK
                     AAALAPDIVLMDVWMPGISGLDAVREIRARGLPCVFILVTAYERFDIAREAMELGVLD
                     YLLKPVSRDKLAAALRAASAFADRRFELERREMEHREREERLRSFVEEAFLDAIRSGD
                     GAEFDPEPYRATLGIRGTRAEVAAAAFLPPPGSPDRRSDIRGLYESFRQAIRYKTRAL
                     AGPLRGGLSIVLLPIEDPDQEAKEAAAFRETLGQSPGIEPGRGSLRLGFAGPAPLAEA
                     PSAWVRAYRESLGLGSPGGEPGPEAPDMPAEARAEDERTFLETLASGDPDRARQALDR
                     ILEGFRGRAEPSPGQRYRLIGLFSEAYRDFERRGFLGSAEAARLQDLEDLRAAPGAEG
                     LAEAARARMPALAAAAEKAPRWSVPVARAAAYIRENYARQISLESAAAEVLLSPGRLS
                     RLFVEETGRGFSDFLTEVRIERAKELLIQPGASVKLVSAVCGYPDPNYFARLFKKVTG
                     YTPSAFASDPREEPDAEP"
     gene            complement(19760..21229)
                     /locus_tag="P5313_05455"
     CDS             complement(19760..21229)
                     /locus_tag="P5313_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRY79844.1"
                     /translation="MRRAGVRGKIILNSALVLLIVASAVLYAGLASVGLARSVEILFR
                     NNRLMEDIRDSLGRTEASLTVYLSTKSSDALKDYIKYSTRLSEDARRLNREIRADEVL
                     LLERDLAGLLDSFLADAEASVASKRGRDVAAYTERFESAERTLDLVMFLIDRIEGVFI
                     SDSLAAFSGFNSRVTSVLVSNAVLVVFATLMGMAILVSFSFRLTEPLAELARAAEALG
                     RGDYDHDLPPPGRPDEIGVMAASFASMRESVRRAFEEQRAKAELERSLMEERVRLLDM
                     DHKLKDAELLALQTQINPHFLFNTLAAGQQLALSEGADRTGDFMENLAAFIRYALKPP
                     SRSVRVSDELECVRLYIWLLRLRFGERYRFEVEADDRALSVETPALVLQPLVENAVGH
                     GLRDREEGGTVRVSARVEGREAVLSVEDSGGGMSPAERERILGEPAGEGDPRAGIGLR
                     NVIRRVTLATEGRGRVDVESSEGAGTAVRIRLPLEGVVP"
     gene            complement(21226..22212)
                     /locus_tag="P5313_05460"
     CDS             complement(21226..22212)
                     /locus_tag="P5313_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY79845.1"
                     /translation="MKRAAWILAALFGAAFLGAILMNARLVDQSRRAFAGGVFRETSR
                     IPKARYHLVLVIPDDDDSFFSGLAEGVRSGAQETDSAVQVFRYAASSPADSERFFEIA
                     LGAGVDGLIMYSPRGEPVLRRAEAALRRGVVFVPVGTDVPEGEPPGFIGSASLLQGFE
                     GGRRICAALGASARIGVILPAGGAGPEEEEPLFRGVSASIQAFPGARVSALTRARPGT
                     LAGEEAASAMLGADPSINAIFCANSRDTMGVAQVIVDLNRVGSILVVGADETPEILRY
                     IEKGVVTASIVRDSKWIGREAVLAFARIKDGGAPAGPVEAGFYVRTAEGRSP"
     gene            complement(22280..>22579)
                     /locus_tag="P5313_05465"
     CDS             complement(22280..>22579)
                     /locus_tag="P5313_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79846.1"
                     /translation="LPRRGFSLPAVLVLEASPDADGRTVRSLLRRLAKLGLETEPGAG
                     DRIFELRLRLSGSGAEFSLEQRASGAVLRRGSVSFGAAGYGAYEVAEFVFREVNK"
BASE COUNT         3305 a         8024 c         7511 g         3739 t
ORIGIN      
        1 ttccccgtga cgccgacctt cgaatccgta gtttcgcccg gattctccct ggacaacggg
       61 gaatacttcg cccggtattc cttcctgggc gaatccttcg accttgcgtt gatgggggga
      121 tggttctgga acgatacccc gggctacacg gtgaccgaca agaccttcgt accgggtgtc
      181 ggcattaccg ccttgaccgt gcggcccgag tactatcgta ccgccgccgc gggatacgcc
      241 gcgtcgggcg ccatgggacc cgtgatcctg aaatccgaag gcgccttcta cgggctccgg
      301 cggtatcagg gaaaccccat gatctacccg gacggttacg ctgaaaagta ttcccttcag
      361 tacatggccg gagcggacgc ctccgcggcc ggatttacct tcggaatcca gttcatacag
      421 gatctgatcc tggaccacga tccggacctg gtcgatccgg agctcaagac cacgatgacc
      481 ttcgttgcga cccggacctt cctgcgggat accctgaccg cggaggtgtt cacctacatg
      541 ggtctcgatc ccctgaacgc actagttaag ccgaaggtta cctgggacgc gacggacgcc
      601 ctggaattct tcctgggggc ctacctcttc ctgggggacg agggggattt cggccggtac
      661 gactcgaacg acggggtcta cctgggggcc aagttcagct tctgacccgg aaaccgcagg
      721 gcccgccgac cgcccggttc ccgcttgcta cttcccggtc cggactgcga tactctcctg
      781 agattgcgag gaggcgctca tggaatcgga catccggctc gaaccgagca tagaggagtg
      841 gcgggaacag cgcggcaggc tcaacgagac ggtgatggcc atggccggac tcgagatcaa
      901 gcgctttttc agcctggacg ggcagaccta tcggccgggg gccctggacg ccaagaccaa
      961 ggaattcctg ggcctcgtcg cctccctggt gctccgctgc gacgactgca tcctctacca
     1021 cacgatccgc tgcaaggagg aaggcgcgac cacggaggag ttccaagaag cttgcacggt
     1081 cggcctgttc gtgggaggct cgatcgtgaa cccccgccta gggcgttacc gtgaagacca
     1141 gctccccgat tcgcttccag gacgcctcgt cccaggaggg tgaatcccga tcccggatat
     1201 acagggtcac gcgatacggg cccggggaac gcggatccag ggggaacacc gtgtcgccgg
     1261 gattgttcac atacttgttg ttccggcccg cggcggcctc cccggtcccg ctgccgtcgg
     1321 gattccgcag tgcatacccg aagacgtaca gaggcattcc gcccgagttc aggaaggcgg
     1381 attggggcgg gccggccagg cgggccccga agatctcccc ccgggcgtag ccctcccgga
     1441 tctcggccag actgaaggag gcccggaccg cgaccggacg ggtctccacg tcgtaggtcc
     1501 ccgggcccga gggcgactgc cggaaaagtc cgatgcccca gtcccgcagg gtccaggatc
     1561 cgtgggtgaa cggcgggacg gagaaggggg agcccgcccc ggatcccccg gggacggccg
     1621 ccttcgatcc gtccgagaag tttaggacgt actcccggga ggcccgctcg accttggctg
     1681 cgttgtcgaa caggacggtc cggattttcc aggccccgaa ggacggagcg gccttgttcg
     1741 ggctcaggtc gaagaagagg ccgggcagga cgaagctcgt gtccgcgtac ccctgaaggt
     1801 cgaaaccgta ttgggtgtcc caggcgatgc ttccgtcggg cctcaggacc tggacacgga
     1861 gataatagtc gaaattgccg taggcgttca ggaagccttc ccgtcgggtc cgcatccaga
     1921 accggacgtt cctctgctcc cctccgcccc gggtgaaggc ccactcccgg acggccggat
     1981 ccatcccggc ctccaggatg ggaggatcca ccgcgaacgc gggcgcgaag gacaacccca
     2041 ggatcagcgg aaggacggtg aacatcctca tagtaagcct ccgtgcctcc attctatcac
     2101 gggaacgggg atcggccgga tacggcgagg ccgagcgggc gccctatatc gccttgacct
     2161 tccggcactc ctcctccagg gcctcgaaac cctcggagag gacacgttcc aggatgcgga
     2221 ccgagacggg gtagacggcg gtctccagga gttcccgctc ggtcttggag aagtccgaga
     2281 ggacatagcc cgcgacgtcg tcatggtcgg gccgcccgat cccgatacgg aatcggagga
     2341 agtccggggt cgagaggcgg tcccggatgg accgcaggcc gttgtgaccg cccaggcccc
     2401 cggcgcgctt caggcccacc gtacccaagg gcagttccaa ctcgtcgtgg accaccagga
     2461 gctccccggg ctcgacgcgg tggaaccgca tgagctccgc gacgctgtcc ccggagaggt
     2521 tcatgaaggt ctcgggggtc aagaggaaca cgcggccgga gggggtatcc agggacgccc
     2581 agcggccctt gtacccccgc tcccagcgta gggagggatg ggccggaagg cgctccagga
     2641 cctgccaggc gacgttgtga cggttgttcc ggtactgccg tccgtgattg cccaggaagg
     2701 cgtagagtcg gaccatccgg aagctccttc ggatcggggg atcggttgcg gccgcagcgg
     2761 aagtcgcgcc ggcccgcgcg gcccggcccc ggattggaca tccgggaccg ggggatagta
     2821 caatgcggaa ccgtccctgg acaagcgagg agccccatga cccccacccg tttcgtgaga
     2881 accctccggt ccgaacgctt cgcggacctc gactgcgccg tcctcgaata ccgctcccgg
     2941 gatggttcca cggacgtcga gccgcggatg accctcctag aggagcttcc cctgatcgaa
     3001 ctcgtccgcc tggctgacgg acccgcagga gggcggccga cggccggtcc cggcaatccg
     3061 gctggtgacg cggccttcga cctgcgcggc gccttcgtct tcgccaacgg ctggcagtcc
     3121 tggtccccgg gctgggagct cgccccgggg gagcggatgg aacgggcccg cttcgtcgga
     3181 cggctcaacg tctttacgga ccacccgggc ctcgtgcccc ggcgcgggga ggtcctggcg
     3241 agtttcctgg gctacctccg gtccgggggc gagtacctgg ccctggcttc cctcccggag
     3301 ggcggtccgc ctttgtcctt cctcttccgg cggcgggaag gaacggtctc tctcctggcc
     3361 tatgccgagg gccaccgctt ccgggccggg gacctggcgg cccggatcct ggtcgtccgc
     3421 gcccgctcct ggttcgccct caaggacgtc ctgcgggagg cctacgcgcc ctacgaccta
     3481 ttctcccgcc tcgccttcct gggagccccc ggggagcccc tggtgcccgg gggctacgag
     3541 tcctggtaca accactacgc ggacatcgac gagggcctca tcctggcgga cctgcggaac
     3601 ctggcgggga acggcaacct catcgaccgg ctctacatcc gaccgggcaa gcccacggtc
     3661 ttccaggtag acgacggctg ggagcggacc gtgggggact ggagggtgaa cgaagcccgc
     3721 ttcccccggg gcatgaagga cctggccgga cggatcgagg aggcgggctt catcccgggg
     3781 ctctggctgg ctcccttcct ggtgacccgc ttcgcacccc tgtaccggga aaagcccgag
     3841 tggctcctcc gggacgagcg cggccgcccc gtggcggccg gctggatccc cgcctggggg
     3901 cacgatttct actgtctgga tctctcgatc ccggaggtcc tggactatct tacgggtatc
     3961 ttcgaccggg cggtgaacga atggggctac cgctacctga aactggattt cctgtacgcg
     4021 ggcatgctcc ggggaagtcg ccggaacgga gaccccgcct ggatccacta ccggaaggcc
     4081 ctggaacgtc tcacctcgat ccggaaccgg aggaacggca agcccgtggc ctacctgggc
     4141 tgcggcgccc ccttcgaagc ctccttcgag ttcctgcccc tgatgcggat cggggcggac
     4201 acccgggagg attgggactg ggcccaggcc cggttcctcc gccaccccgg acggccctcc
     4261 gcctggatct cgatgaagga caccatcgga cgggccctct gggacgggac ggtcttcctg
     4321 aacgatcccg acgtggtctt ctgccgtacc cgccgcatgc gcctttccga aaccgaaaaa
     4381 gagctcgtgg ccctgacggc ccggatcttc gcctcccagg tgatgttctc cgacgacacc
     4441 gcggacttcg agcccgcgac cgagggggcc ttcacggagc ggatcgcgga gctgtacgga
     4501 cgtatatccg gccgggagtt ccgccccgaa cgcgtcccgg gcttccggga cgtctacacc
     4561 gcccgtagcc gggacggccg gtccgcggcg gtcctcaacc tctccgactc ggacgccgtg
     4621 gttccgggct cctggcccgc ggacaaggcg gtcctggccc ggttccggag cgaaggcggc
     4681 cggacggtgt tcgccccccg ttccatcagc ctcttcgacg gactggagga agcatgaagc
     4741 gcacggtcta cgtgatcggc cacaagaacc cggacaccga ttccgtcgtc tccgccctgg
     4801 gctacgcccg tctcaagcgg gagctcggga tggccgaggc gacggcagcc cgggccggga
     4861 tgatcaaccc ccagacggaa tacgtgctgt cgcgcttcaa ggtcgaagtc cccgccttcg
     4921 tccccgacct ggtccccaag gcggagtact acctggacga cgccgcggtg accgtcccgg
     4981 cgggcacccc cctctgggac gccctggccc tgatggagga gcacggccgg accgccctgc
     5041 ccatcgtgga cggccagggg cgctaccggg ccaccctcca ctactccgcc ttcgcccgca
     5101 acatcctcaa gaagatcaat ccccggaaga aggccgtgat ccccaccagc gtggggagga
     5161 tggccgccac catcaaggcc caggtggtga cctccttcga tcccgagacg gagttcaagg
     5221 cccggatcct ggtggccgcc ctggagaccg aatccttcaa gcgccacctg gacggggagg
     5281 cccgggagaa ctgcatcgtc atcgtgggag accgggcgga cgtccagcgc tacgtcctgg
     5341 agtcgaaggc ccgggtcctg atcgtcacga acggcgccgt cctggaccgg tccctcaagg
     5401 agatcgcgga gcgcaaccgg gtgtccgtcc tgctctcgcc ctacgacacc agctccacgg
     5461 ccctcctggt catctactcg acccccgtgg agaccatggg ggacgagggc ctgaagcctg
     5521 tgcgcctgga ttcccccctg cggaacctgc ggagccccct ggcggagagc cccagtcgtt
     5581 cggtccccgt cacggacgag gagggccggg tggccggcat gttcaccgag ggggacctcc
     5641 tccgggaccc gaaggtggag ctgatcctgg tggaccacaa cgagctgggc caggccgtgg
     5701 agggggcgga gaactaccgg atcctggaag tgatcgacca ccaccgcatc ggctccttct
     5761 cgacccggtt cccgatcacc ttcatcaacc gggtggtggg ctccaccagc accatcgtgg
     5821 cggggatgta ccgggagcac cggatacccc tgcccaagcc gatcgcctcc atcctcctgt
     5881 gcggcatcct ctcggacacc ctggtcctgc gatccgtgac caccacggag acggaccggg
     5941 agaccgccga gtacctggcc gccatcgcgg acctggacat cccggccctg ggcgcggaca
     6001 tcctggccgc ctccagcgcg gtaagctcca agcccgcccg ggacctggtg cgcctggaca
     6061 tgaagaccta caccgtgacg gaccggacct tttcggtctc ccaggtggaa gtggactccc
     6121 ccgaggacct cctggcccgc cgggaggagg tgctccggga gctggccgcc ctccggtccg
     6181 agggaggccg atacctgtcg gccctgatgg tcacggacat cacggagctc tcctcctacc
     6241 tcttcttgtc ggcggacaag gagttcgaga gcctggtgaa gtaccccgag gaggaggacc
     6301 acgtctaccg cctcaaggac atcctctccc ggaagaagca gctggtcccg gtgctcctgg
     6361 agctggcgga aaaggccctg ggggcctaac cgagtgttga aatgcttcga gtatttcaac
     6421 aacctgctag acccgcgtcg aaggaacccg gccggaatct tttcttgacg gacccgggac
     6481 cccgggtata ctggtacttc ccatgaagct gctggtattc gtcttgaaca aggaagagct
     6541 cctcgaccgg gtcctggaag cctacatgga ggccgggatc accggcgcca cgatcctgga
     6601 ttccgaggga atggggcact tcctggtcta cgaggtcccc ctcttcgcgg acttcaagga
     6661 cttcctgaag gggcacaagc cctacaacaa gaccatcctc tccgtggtcc ccgacgagag
     6721 cgccatcccc cgggtggtgc gcctgatcga caaggtctgc ggcggcttcc ggaattccaa
     6781 cacgggcatc ctgttcacgg tgcccgtgga tttcgccgtg ggcctgctgg aacagaatcc
     6841 cccgggaccc gaggagacgc cgtcatgacg gacctcagcc ccttcttcac cccccagacc
     6901 gtggtcctgg atttttccgc ccgcaagaag ggggacgtac tccggaaggt ttcccagatg
     6961 ctcgccgggc cgggcacggg gaaggccgat tccctggagg acgccctcca gcgtcgggag
     7021 atcctgtgtt ccacgggcct cggcgaagga gtcgcggccc cccacgtcct cctgccgggc
     7081 ctcgaaaaga cctccctggc cttcctccgg ctccgggacc cggtggattg gagaagcccc
     7141 gacggaatcc ccgtccggct ggtcttcgcc ctggccggtc ccgctgagga ccctgccgcc
     7201 cacctccaga tcctgtcccg gctgacccgg ctcctccggg aaccggactt ccgcgaaacc
     7261 gtggccgccg ccgcatccgg cgaggaggtc gcatccgcct tccggcggac cgaggaggaa
     7321 tagggcatga caccccggac caaatgggac ctcgtgcgct tcgtctccac cttctcggtc
     7381 ctcctggcct cctggctgct cttcgcgggc cccccggacg cctactccat cgccgccggg
     7441 accctctccg cggcggtcct ggcggccctg acctaccggt tcttcctgga cgaccacgag
     7501 gccggggccc gccacctgat accgaacccc ctgctcctgc tcctgtacct gccgtacata
     7561 ctgttccgga tctacgcggc cagcttcatc ctcgtctacg acatgatagc gggaaggatg
     7621 aacccccgga tcgtccactt ccggacccgc ctgcgctcgg acctggcccg ggtcgtcctg
     7681 tgctcctccg tcaccctgac ccccggcacc atcgccgtcc tgatcgacga ggaccacctg
     7741 ctggtgcact ggttcaacgc cgagtccggc cactccggac gggcccggcg ggagatcgcg
     7801 gagtccatgg agcggttcct gggaaggatc tggtcgtgat gccctgggat ttcgtcctct
     7861 ggttcttcct gggttccctg gtcctgggcc tggtgcgcct ggtcatcggc ccttccacgg
     7921 cggaccgcct ggtgggcctg aacctggtgg ccgggcaggt cctggccctg ctcgtgctcc
     7981 tggcggtcaa ggaggacgtg gccctgtacc tcgacgtggc cctggcctac gacatattcg
     8041 gattcctggg catcctggcc atcgtgaagt acttcacgaa acggagggcg tcatgaaccc
     8101 ggcctactgg atggaattga tcctgatctt cctggggatg ggcttcgccc tgatcgggaa
     8161 cctcggggtc ttcctcatgc ccgatcccta cacccggctc caggcttccg cgacctgttc
     8221 caccaccagc gtgatctccg tcatcctggc ggcgatggtc tcctcggggt tctcccccac
     8281 cacgggcaag ctggccatca tcctcctcta tttcctggtt tccagccccg tggccacgac
     8341 catcatcgcc cggtatgcct gggagtccgg gatcgtcccc tggcgcaggc ccaagagaag
     8401 gcttggaaaa ggaggatccc catgatcgag gcctccctcg tcctgatgct cctggtcgcg
     8461 atcctctccc tggcggcccg ggacctccta gccacgatcg tgctcctggc ggtattcagc
     8521 ctcctgtgcg ccgtggtttt ctactacctg cacgccccgg acgtggccat caccgaggcc
     8581 gccgtgggcg ccggggtctc caccctgatc ctggtctggg cggtccgccg caccgcccgc
     8641 ggggaggaag gatgaggacc ctgcggcgcg tcctggaact ccttcccttc ctggccctgg
     8701 ccgtggcggt catccttccc ctggccgtga tgcccccggc cccgacggcc ttgcgggact
     8761 actgcgtgga acgggggatc gccgagaccg gggcacccaa tctggtcagc gccatctacc
     8821 tgggctggcg cgcctacgac accctggggg aaaccatcgt cctcctgctg gccgtgacgg
     8881 gcgccctcta cctactggga ggccgggaat gaggaaaacc gacgccctct ccgtgataac
     8941 cggcaagctg ggtcccttgg tcctggtgtt cggggcctac ctgatagcct tcggccacgt
     9001 ctccccgggg ggcgggttcc agggaggggt cgtcctggcc agcggggtcc tcctcctggc
     9061 catcggccgc ggcacggaca ccgtgctcgc cctcctgacc ccccggctcc tggcccggat
     9121 cgaggcctcc gccttcgccc tgctcctggt gaccggactt gcgggcatgc tggcgggcaa
     9181 gggattcttc gggaacttcc tgaccgccgg cggcccggcg ggcccggtcg gattcatcat
     9241 cgccctgaac gtgctgatcg gggtgaaggt cggggccggg gtggcctacc tctgcctgat
     9301 cctgctgaag gactcggacc gatgatcccc cgtctgctgt gcggagccat cctcctgacc
     9361 gggctcgcgg ggctcgtctg gaaccgcaac ctgatcaaga aggtggcggc cctctcggtc
     9421 atgaacagcg cggtcatcct gctcttcgtc ctgggcgggt ccgagacggg gatacggtct
     9481 cccatcctga cggggaccga cctgcccgtc gtggatcccc tgccccaggc cctcatgctc
     9541 acggccatcg tcgtgggcgt cgcggtcacc gccctggccc tggtgctggc ggtccgcctg
     9601 cacgagcgct tcggcaccct ggattcccgg gagatcgagg agaggatacg ggatgagccc
     9661 tgagcggctg gccctgtgga tcgccctcct gcccctcctg gccgccgccc tgcccctcct
     9721 ggagaaggcc ttcgggcggc cccggaccga agccggtccg gcgagcgacc tgggcgccct
     9781 cctcggcctg gccggaccgc tggccgtcct ggccctgctc taccccgccg tgacggacgg
     9841 ggggatcgtc cacctgaccc tgggcgagtg gtccccgtcg atcggcatcc ggtatcgcct
     9901 ggacggcccg gcctggttcg cctctgcctt cacggcggtc gtgtccctgt gcgcggggct
     9961 ctacgcccgg gcccggggag ggtattcccc caccttctgg ttcctcttcc tcctgtgcca
    10021 ctggtccctg cagtccgccc tgctggccgc ggacttcttc cacctcttcg tctgcctgga
    10081 gctcatggcc ctgacctcct acgtcctgat cgcctacaag aaggagagcg cggccctctt
    10141 cgcgagcttc aactacttga tggtgtccac cgccgccatc gtcttctatc tcttcgggct
    10201 gtacctgctg tacggagcct cgggcaccct gtccatcgag gaggccggcc gcgtcctggc
    10261 ctcggcgaga cctcgggagc ggggcctcgc ggccctgggc ctggccttcc tggccggggg
    10321 cctgggggtg cggaccgcct tcctgccctt ccacggctgg ctaccggacg cccacgccag
    10381 cgccccgcat cccgtatccg ccctcctgtc gggggccatg atcaaggtga gcttcttcgc
    10441 ccagtggcgc atcctttccc tggcggagtt cccgatgctg cgggagagct tcctctggct
    10501 gggggcggcc ggggcgtgcg cgggcgtggt attggccctc tgccagtccg acgccaagcg
    10561 cctcctggcc tggcattcgg tcagccaggt gggctacgtc ctggccgcct tcggggccgg
    10621 gagccccgcg ggcctcctgg cggcgggcct gcacgccgcg gcccacggcc tgttcaagag
    10681 ccttctgttc ctctccgtgg ggagcgcggc ggacgccgcg ggatcccgga acgcgtaccg
    10741 catggcgggg gctttccggg gcctgccgga ggcggctgcg ggattcgtct tcggcgcctt
    10801 gtccatcgcg gccctgcccc ccttcatcgg ggcggtgagc aaggacctcg tggcctacgc
    10861 cgcgggaggc ggcctgccgg gagccctcct cctggcggcc tccgtgggca ccgcggcctc
    10921 cttctccaag ctgggcatcc tcttcttctc ggcgccggaa ccgggatccc ggaccccggc
    10981 cctttccgcc gtagaactgg gaaggctccg ggcggccctc cgccggggag ccctgcccct
    11041 ggcggccctc tgcctgggca cgggcctcgc cgctccggcc ctggtgcccg ccgcggccgg
    11101 gctcctggcg ccgggatata ccgcggcctt cgccttttcc ctttccgggg ccgcgaagag
    11161 cctggggatc gcggcggccg gcttcgccgc ggcccgggcg gtcctctccc cccggggccg
    11221 ggccctggcc caccggttcg agcgcctggg taccggatcc gaaaccaccc tgaccctgac
    11281 cctcctgggc cttgccctcc tggcggccct gggcggctgg gcggggcggg gaatcccggg
    11341 gtagcccacg atgaccgcac cgggtcccga ggtgacggcc gttctggtcg tgttcctggc
    11401 ccccctcgcg gccagtctgg gtcccctggt ttccaaggcc ttcctggacc ccggtctccg
    11461 aggcggctgg gcggctcgag ccatcccggc ggtctgcctc gttctgggtc tcctggcgtc
    11521 cccggcggcc ctgatccgcc tccatgccgc ggtgaccggc ggcggaaccg tggccttcgc
    11581 cctgggtctg ccctctcccc tggccatccg gtacctcctg gacggccccg cctggctggc
    11641 ctgcgccctg atcgcggcga tcgggctgtg cgcgggcctg tacggcctgg tctcgggggg
    11701 atattccccg gcgttctggt tctttttcct cgtggccctc tcggccctct acgcctgcgt
    11761 cctgaccgcg gacctcttca acctcttcgt gagcctggaa ctcctggccc tctgctccta
    11821 cgttctcatc gcctacaaaa ggacacccgc gtccctctgg gcgtcctatt cctacctggt
    11881 gacctccacg gtggccgtgg tgttctacct gctgggactc tacttcctgt acgggtacgc
    11941 cggaaccctg tccatcccgg aggcggccgc gtccatccag tcgctgggtc cggtccctcc
    12001 ccgggatctc cggatggccc tcgggttcct gaccgtggcc ctgggggtac ggagcgccta
    12061 cgctcccttc cacgcctggc ttcccgaggc ccacgcctcg gccccccacc ccgtctcggc
    12121 cctcctgtcc ggggccaccc tggcggcggg gtatttcgcc ctgggccgga tcctgtcgct
    12181 cctggacgtc cccgccctgc gggagggtct gctctgggcc ggtgccctga cggccctgac
    12241 gggaggcctg ttcgcggtcg cccagcggga tgccaaacgc ctcctggcct ggtccagcgt
    12301 gggacaggcc gggctctgcc tggcggcctt ggcctcgggc tccttcgcgg ccggggccct
    12361 gcacgccctg gcccacgggg cggccaaggc cctgctcttc ctgtcggtgg gccgggcggc
    12421 ggacgaggcg gggagtcggg acctcggcat cctccaggga cgggccggcc tcggcccctc
    12481 cctgggtatc ctgatcgggg cagcctccct ggcgggcctg ccgcccctgg ccggctgggc
    12541 ctccaaatcc ctggcgggct acgcggcggg cggcggcgcg gccgggatga tccttgccgc
    12601 cgcctccgtc ctttccgcgg ccgcggcctt ccgggtcctc ccgctcctcg taatcttccg
    12661 cctctccgga ggtccgcggg aagggtcccc cgcgccgtcg ggtcccggag acatccggaa
    12721 cctcgccgtc gttctgacgg ggctcctgcc cctggccgtc ctgtgcgtcg ccgggggtct
    12781 gcccggcatt ccggcggccg cggcgtccct ggccgattcg ggggccggcc ccctcggcat
    12841 cccggcgttc ggatggcccg ccctggccaa ggctctgccg actctgggcg cggccgcggt
    12901 tatagcggcg gccgccggaa cgcggcccgc gaggaggatc ctggatcttt gggaacggag
    12961 gggcaccggc gcggacgccg ccctcagcct gctcctggcg ggggcggccg cggccgcgct
    13021 ttccggactg ctctaacttt ttgccttccg ggtctgcacg tcgaagatga cggccgccac
    13081 gaggacggcg ccccggatga tgtactgcag ggagatgtcc gtgcccagga ggttcatccc
    13141 gctggtcagg gacatgtaga cgatggctcc cacgatggaa cccgtcacct tgcccacccc
    13201 gcccgcggcg gaaacgccgc cgatgtaggc cgccgcgatg gcgtccagct cgaagagggt
    13261 gcccgcggtg gtggtggccg actgcagacg ggacgcgaag aggacccccg aaagcccaca
    13321 gagcatcccc atggacccga agacggtcag ggtgatcttc ttaacgctca cccccgagag
    13381 ctccgcggcc tccggatttc cgcccaccgc gtagatgtgc cgtcccagga ccgttcgggt
    13441 ggtcacgaag ttgtagatga gggccacgac gatgacgatg gccagggtcc aggacatccc
    13501 gttgtacccc gccacgaccc aggtgatcag ggacaggact acggagagga agagctgctt
    13561 cagggcgaac atgtccccgg gcaggacttc gagcccgtac ttctggcggc tggcgcgctc
    13621 ctggaaagcc cggacattga gccacacgat ggccaggatg cccaggagga gggtcagctt
    13681 gtggagctcg ggcaggaagg ccgtgtccgc gggcaggatg tccgggatgt agccgttgcc
    13741 gatggcgttg aagtcctcgt tggggatgat gatcgtgccg gtcttgaggg tgaccgtgag
    13801 gagggccccc cggtagatca gccagcccgc cagggaggcc acgaaggcgg ggatccccac
    13861 ccccgccacc aggtatccgg tcaacagccc cgccagggtc cccaaaacca ggacgatcag
    13921 gatggcgatc caggtggaga ctccccaggc ggtcatcgcg atggccgcca cggctcccag
    13981 gaagcccgag aggaagccga tggacaggtc gatgtgccgg atcacgatga ccagggtgat
    14041 cccgatggcc aggatcgcga tgtaccccgt ctggttcagc aggtttgcga tgttccggga
    14101 ggagatgaag attccctggg tggcgatcgt gaagatcccc atgatcacca cgagggcgat
    14161 gtacatgccg tattcgcgaa tgttgctccg cagggcggag cgcaagtcct tgagtatcac
    14221 cgttccctcc tcagctcgtc gccagccgca tgatcttctc ctggtccgct tcctccaccg
    14281 gaagctctcc ggtgatccgg cccgtggaga cgatgtagac gcggtcgctc atgcccagca
    14341 cctcgggcag ctccgatgaa atcatgacga tgctcatgcc ctcggccacc agcttgttca
    14401 tcagggtgta gatctcgtac ttggccccca cgtcgatgcc ccgggtgggc tcgtccagga
    14461 tcaggacctt gggccgtacg aagagccact tggctaccga gaccttctgc tggttccccc
    14521 cggagaggtt caggaccttc tggtcgatgc tgggggtctt gatggacagg gcggaacggt
    14581 aatcctccgc caccttggtc tcggcgttgc cgtccaccac cccccgggcc gccagggccc
    14641 gtagattggc cagggtgatg ttctgcttga cgtcctggat caggatgagc ccgttccgct
    14701 tgcggtcctc gctggcgtag gcgacccccg cgtcgatggc cgcccgggga tccccgaagc
    14761 ggacgggcct gccctccacc aggagctcgc cctccacccg gtacccgtcg gggttgccga
    14821 agacgctccg ggccagctcc gtgcggccgg aacccatgag tcccgcgaag cccacgatct
    14881 ccccgcggcg gaccgtgaag ttcacgtcct tcaggaccgt gcgccccagg ccgtgggaat
    14941 agaccgtcca gttccgggcc tccaggacca cgtccccgaa tttcttcgag gtgcgcttgg
    15001 gatagacgtc gtcgatctcc cggccgacca tgtgctggat gaggatccct tcggacacct
    15061 cccccttgcc ggcgtccagg gtgcggaccg tgcgaccgtc ccgaaggacg gtgaccgtgt
    15121 ccgcgatgcg gatgacctcc ttgagcttgt gggagatcat gatgcaggtg accccgtccc
    15181 gcctgaggcc ccgcaggatg tccaggaggt tgtcgcagtc gtcctcgttg agcgccgcgg
    15241 tgggctcgtc caggatcagg agcttcacgt cccggctcag ggccttggcg atctccacga
    15301 gttgctgctt cccgactccc aggtccctca ccttcgtgga gggatccagc tggagccgga
    15361 ccttggccag catctccgtg gccttcttga tggttccgtt caagtccacg gtcaccccgg
    15421 accgcagttc gtggcccaga aggatattgt cgtataccgt catgtccggg accagggcca
    15481 gttcctggta gatgatgccg atcccggccc gctcgctgtc cgcgatgccc cggaaccgct
    15541 ggggcgatcc ggaaaggagg atctccccct cgtaggatcc gtggggatag actcccgaga
    15601 ggaccttcat cagggtggac ttgcccgccc cgttctcccc caccaggcag tggatctccc
    15661 cccgggccac ccggaagctc acgtcgctca gggcccgcac cccgggaaag gtcttggtga
    15721 tgttcctcat ctcgaggatg ggttcgctca tcttccggcc tccgttcggg atccgcactc
    15781 cgtccgcgga agagggcgcc gacggccggg ccgccgacgc cctccgggcg tttcatgcta
    15841 ggttcttatt atagtccctt gaagtcggaa gcgggatagt agccggagtc gacgatggcg
    15901 gccttcacgt tcgccttggt gaccgtggtc gtggccgacg gcttggcggg gatttccgcc
    15961 cggccgttgt tgtaggactt ggtcttcgcg ggagtcccgc ccttgaggta ggcgacggcc
    16021 gcggcgatcg cgtcggcggc caggacccgg acgtccttga ggaccgtcat ggactgcttg
    16081 ccgtcgatga tgtactgcac ggaggccttt tccgcgtcct ggccggtgat ccagtacttg
    16141 gtcacggccc ggtccacggc gaaggcgtcc gcgatggcgc gggcggtgcc gtcgttcggg
    16201 gcgcagatga agacctcgcc cttcatggct ccggtggccc gggtcaggtc ggcctcggcc
    16261 ttgctcttgg cggtgttgaa gtcccagttg gtcgtgacct ggccgatgat cttgcccatc
    16321 tcctcacggg agagcttggc cttcttgctc tgggccacgg cctcggtgga gttgcggacc
    16381 acgaaggttc cgtccgcgat cttgggctgg agcttgttcc aggcgccctc gaagaagagg
    16441 aaggcgttgt tgtccgaggc cgcgccggcg tacaggtaga ggttgagtcc cttgcgggtc
    16501 cccgcctggc tgaccaggaa gtcgccccag gcctcgccca cggccacgga gtcgaaggtt
    16561 acgtagtagt ccacgctggc ggttccggtg atcaggcggt cgtaggagat gaccttcaca
    16621 cccgctttcc gggcggcgtc cgcggcggcg gccgcggcgg cggaatcctg cgggcagatg
    16681 atcaggacct tgatcccctt ggtgatcagg gactcgacgt tggccttctc cttggcggag
    16741 tccccctggg agaagaggat ctccacgttg tacccggcct tggccagggc gtccttgaac
    16801 cgggcctcgt cctggagcca gcgcggctcg tccttggtcg gaagtacgat gcccacgggc
    16861 aggctccccg cctgggccac cgcggagacg acgatgcccg ccaggaacag gacggcggac
    16921 aggatggcga cgatacgctt catacaaacc cccttactgc gactcgcggc cgacgggccg
    16981 ctggcctaca gtatccgggg ctttttacga tcggtcttca ggaatgttcc ggcgaaattc
    17041 cgccctgccg ggatttcttg gattcccgga gccctgctag cactcgggct cggcgaatgg
    17101 tggaaaaatc cagatcgggc cggcccccgc cccccgcgcg gacagcctac ctccggacct
    17161 tcctgtacac gtctgcggag gagtggaacc cgtccttgat caccgtcgcg tccaggagat
    17221 cccgggtgac caggatgggc tccagccgga cgtagggcac ctcgtacttc ccgtcgttga
    17281 tcacggcgtc cgtccggatc tcctcgcccc gggccagcat gaccgcgtag cccgcggcct
    17341 tgagggccag gcggtcgatg ggcttgtaga ccgtggcgaa ctggaccccc tcggcgatgc
    17401 gctggcaggc cgccagctcc gcgtcctggc cggcgatttt cacgttcccc atgagccggt
    17461 tttccgagag gaccgagatg gccgcctcgg ccagcatgtc gttccccgcc accaccgcgt
    17521 cgatgccccg gtcgtgctgg acgatctttt ccagggccga cttgacctcc tcgctgtccc
    17581 aggtcccggg ccagatctcc cccacgatgc gcaccgcccc ggaggcttcc aggggatcca
    17641 ggaccccccg gatcccggcg ttcagcatca gggcgttgtt gtccgaccgg gccccgttga
    17701 tgatcacgaa ccctccccgg ggcacggccc gggacacgga ctccgccatc aggcggccca
    17761 ccatcacatt gtcgaaagag acgtagaggt ccacgttcgc gttccgcacc agccggtcgt
    17821 agctcaggac ccggatgccc cgggcccgga tctcggagat caccgaggat agccggtccg
    17881 cgtcgttggg caccaccacc aggacgtgga tgcccgcgtc cgcgaggtcc cggatctgcc
    17941 gttcctgcac cgcgtggtcc tggtccgcca cgcggagcag gacgtccgcg ccgagctccc
    18001 gggcggagcg gatgaagatc tccgcgtccc gtttccaccg ctccacgacc agggaatcca
    18061 gggaaaagcc gatcaagggc gcctgccccg gttccgccct ccgcggagcg ggcctgcagg
    18121 acaccagcgg aagcgtggcg gacaggaggg cggccgcgag gacaagggcg cgccgggatc
    18181 ggtcagggtt ccgcatcggg ttcctccctg gggtcggacg cgaaggccga gggggtgtac
    18241 cccgtgacct tcttgaagag ccgggcgaag tagttggggt ccggatagcc gcagaccgcg
    18301 ctcacgagct taacggaggc tccgggctgg atgaggagtt ccttggcccg ttcgatccgg
    18361 acctccgtca ggaaatccga gaagccgcgg cccgtctcct ccacgaagag ccgggaaagg
    18421 cggcccgggg acaggagtac ttccgcggcc gcggactcca gggatatctg ccgggcatag
    18481 ttctcgcgga tgtacgccgc ggcacgggcc accgggacgg accaccgggg cgccttttca
    18541 gcggccgcgg caagggcggg catccgggcg cgggccgcct cggccagtcc ctccgccccc
    18601 ggagccgcgc gcagatcctc caggtcctga aggcgggccg cctccgcgga accgaggaac
    18661 ccgcgccgtt cgaagtcccg gtaggcttcg gagaagagcc cgatcagtcg gtaccgctgc
    18721 cccggggacg gctccgcgcg gccccggaaa ccttccagga tgcggtccag ggcctgacgg
    18781 gcccggtcgg gatccccgga agcgagggtt tccaggaatg tccgctcgtc ctccgcccgc
    18841 gcctcggccg gcatgtcggg ggcttcggga ccgggttctc cgcccgggga gcccaggccc
    18901 agggactccc ggtaggcccg gacccaggcc gacggagcct cggccagggg ggcggggccc
    18961 gcgaacccga gacggagcga tccccgaccc ggctcgatcc ccggggactg cccgagggtc
    19021 tcccggaacg cggcggcctc cttggcctcc tgatccgggt cttcgatcgg cagcaggacg
    19081 atcgacagtc ccccccgaag ggggcccgcc aaagcccggg tcttgtagcg gatcgcctgg
    19141 cggaaggact cgtagagccc gcggatgtcg gaccggcggt ccggggaacc cgggggcggc
    19201 aggaaggccg ccgccgcgac ctcggctcgg gttccccgta tcccgagggt ggcccggtag
    19261 ggctccgggt cgaactccgc gccgtccccg gaccggatcg cgtccaggaa ggcctcctcc
    19321 acgaaggacc gcaggcgctc ctcccgctcc cggtgctcca tctccctccg ctccagctcg
    19381 aacctgcggt ccgcgaaggc cgaggccgcc cggagggccg cggccagctt gtcccgggac
    19441 acgggcttga ggaggtagtc caggaccccc agctccatgg cttcccgggc tatgtcgaat
    19501 cgctcgtagg cggtgaccag gatgaagacg caggggaggc cccgggcccg gatctcccgg
    19561 accgcgtcca ggcccgagat cccgggcatc cagacgtcca tgaggacgat gtccggggcc
    19621 agggccgcgg ccttctccac ggcgtcccgg ccggaggagg cgtttccgac cacgctgaac
    19681 tcggcggaca ggtccctccg gaccatgagc tcgatggtct ccaccaccgg gcgctcgtcg
    19741 tccaccacca gcacgcgctt cacgggacca ccccctccag gggcagtcgg atccggaccg
    19801 ccgtcccggc cccctccgag gactccacgt ccacccggcc ccttccctcg gtggccaggg
    19861 tgacccggcg gatgacgttc cgaagcccga tcccggcccg gggatccccc tccccggccg
    19921 gttcgcccag gatccgctcc ctctcggcgg gactcatccc tccgccggag tcctcaaccg
    19981 aaaggacggc ttcccggcct tccacccggg cggagacccg gacggttccg ccctcctccc
    20041 ggtcccgaag cccgtgcccc acggcgtttt ccaccagggg ctggaggacc agggcggggg
    20101 tctcgacact cagggcccgg tcgtccgcct ccacctcgaa ccggtaccgt tccccgaagc
    20161 ggaggcgcag gagccagatg tacaggcgca cgcactcgag ctcgtccgag acccggacgg
    20221 accgggaggg gggcttcagg gcgtatcgga tgaaggccgc gaggttctcc atgaagtccc
    20281 cggtccggtc cgccccctcc gagagggcca gctgctgacc cgccgccagg gtgttgaaga
    20341 ggaagtgggg gttgatctgg gtctggaggg ccaggagctc cgcgtccttg agcttgtggt
    20401 ccatgtccag gagccggacc cgctcctcca tgaggctccg ctccagctcc gccttggccc
    20461 tctgctcctc gaaggcgcgg cgcacggact cgcgcatgct ggcgaaggag gcggccatga
    20521 ccccgatctc gtccggccgg cccgggggcg gaaggtcgtg gtcgtagtcg ccccggccca
    20581 gggcctcggc ggcccgggcc agctcggcca ggggttcggt cagccggaag gaaaagctca
    20641 ccaggatggc catgcccatc agggttgcga agaccaccag gaccgcgttg gataccagga
    20701 ccgaggtgac ccgggagttg aagcccgaga aggccgccag ggagtcggag atgaagaccc
    20761 cctcgatccg gtcgatcagg aacatgacca ggtccagggt acgctcggcg ctctcgaaac
    20821 gctccgtgta ggccgccacg tcccggcccc gcttggacgc cacggaggcc tccgcgtcgg
    20881 ccaggaagga atccagaagg cccgccaggt cccgctccag gaggaggacc tcgtccgccc
    20941 ggatctcccg gttgaggcgg cgggcgtcct cggagagccg ggtggagtac ttgatgtagt
    21001 ccttgagggc atcggagctc ttcgtggaca agtacaccgt caggctcgcc tcggtgcgcc
    21061 ccagggagtc ccggatgtcc tccatgaggc ggttgttccg gaagaggatc tccacgctcc
    21121 gggccagccc caccgaggcc aggcccgcgt agaggaccgc ggaggccacg atcaggagga
    21181 ccagggccga attgaggatg atcttgcccc ggacgccggc ccgtctcacg gggacctccc
    21241 ctcggcggta cgcacgtaga accccgcctc cacgggtccc gcgggggcgc ccccgtcctt
    21301 gatgcgggcg aaggcgagca ccgcctccct gccgatccac ttggagtccc ggacgatgga
    21361 ggccgtcacc acccccttct cgatgtaccg gaggatctcg ggagtctcgt ccgccccgac
    21421 caccaggatg cttcccaccc ggttgaggtc gacgatcacc tgggccacgc ccatggtgtc
    21481 ccgggagttg gcgcagaaga tcgcgttgat cgaggggtcc gcccccagca tggccgaggc
    21541 cgcctcctcc cccgccaggg tgcccggccg ggcacgggtc agggcggaca cccgggcccc
    21601 cggaaaggcc tgtatggagg cggacacgcc ccggaagagg ggttcctcct cctcggggcc
    21661 cgcgccgccg gcggggagga tgaccccgat ccgggcggaa gcccccaggg ccgcgcagat
    21721 gcggcgcccc ccctcgaagc cctggagcag ggaggcggag ccgatgaagc ccgggggctc
    21781 tccctccgga acatccgtgc ccacgggcac gaacacgacc cccctccgta aggccgcctc
    21841 ggcgcggcgc aagaccggct cgccccgggg ggagtacatg atgagcccgt ccacgccggc
    21901 gcccagggcg atctcgaaga accgttccga atccgcgggg gaggaggccg cataccggaa
    21961 aacctgtacc gccgaatccg tttcctgggc ccccgagcgg accccctcgg cgaggcccga
    22021 gaagaaggaa tcgtcgtcgt ccggaatgac caggaccagg tggtaccggg ccttggggat
    22081 cctggaggtc tcccggaaga cgcccccggc gaaggccctc cggctctggt ccaccaggcg
    22141 cgcgttcatg aggatggctc ccaggaaggc ggccccgaag agggcggcca ggatccacgc
    22201 ggcgcgtttc atcggggaca gtatacagga cacggacgac gggagccagg acgcgggcac
    22261 ccggtccccc gggacccgat cacttgttca cctcccggaa cacgaactcc gccacctcgt
    22321 acgccccgta gcccgcggcc ccgaaggaca cggatcccct gcgcaggacg gctcccgaag
    22381 cgcgctgttc caaggagaac tccgcgccgg atccggagag gcgcaggcgg agctcgaaga
    22441 tccggtctcc ggccccgggt tccgtctcaa ggccgagctt tgccaggcgc cggagcaggg
    22501 aacggacggt ccggccgtcc gcgtccgggg atgcttccag gaccaggaca gcgggcaggg
    22561 agaagccccg ccggggtag
//
LOCUS       DAOTEI010000037        22395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1521_length_22395_cov_5.457252, whole genome shotgun sequence.
ACCESSION   DAOTEI010000037 DAOTEI010000000
VERSION     DAOTEI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 22395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22395
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_22395_cov_5.457252"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="P5313_05470"
     CDS             complement(<1..195)
                     /locus_tag="P5313_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY79847.1"
                     /translation="MILARVVASVVSTEKDPRYTGKKILAVRPVDPDGSFRGRTLLAV
                     DGVQAGIGDLVLVVDEGGSAR"
     gene            complement(192..470)
                     /locus_tag="P5313_05475"
     CDS             complement(192..470)
                     /locus_tag="P5313_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY79848.1"
                     /translation="MKLGKVIGRVVSTRKLECLEGLKLLLVQPVDETGASAGPPLVAF
                     DAARAGEGDLVIFESGKEAAQANPNGWFNPADAAILGVVDAVDRGGAP"
     gene            complement(482..1921)
                     /locus_tag="P5313_05480"
     CDS             complement(482..1921)
                     /locus_tag="P5313_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HRY79849.1"
                     /translation="MAYGAEDVRRVVEEILRSPEIAALLSSGTKGGSAAASRGPGIFP
                     DMDSAVQAAQAAQRELVRLPLDTRRAVIDAIRRTVLEANEALSQAAVAETGLGNVRDK
                     TVKNRLAALRTPGVEDLESRVVTDEHGLTLTELAPYGVIGAITPVTNPISSIASNAIG
                     MVAAGNSVVFNVHPGAKGVSRLLIEMLGGAIVRAGGPGNLLCAVAEPTLETAGELMRH
                     PGVALLVVTGGPGVVRAAMASGKKAICAGPGNPPCVVDETADLAKAGKDIVDGAGFDH
                     NVVCICEKEILAVASIADRLKEELLRNGAYELKGGQIDAVTKLVLAEPGGPGRPGSPN
                     RAFVGKSPAHIARAAGIEVPEGTRILLLETTSGHPLVWTEQLMPVIPFVRMRNADEAI
                     EFAVLVEHGYRHTAIMHSHDVARLSHMARAMDCSLFVKNGPCYAGLGEGGAGHASFTI
                     ASPTGEGITRPTSFTRERRCTLVDYFRIV"
     gene            complement(1922..2224)
                     /locus_tag="P5313_05485"
     CDS             complement(1922..2224)
                     /locus_tag="P5313_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY79850.1"
                     /translation="MTLARVRGTVVASRRSDGLAGARYLLVEDCGPEGAGRGDYLVAL
                     DMVGAERGQLVLLTQGSSCRWTYRTENAPMDTLAVGIVDAVDSGGATLWNESERGR"
     gene            complement(2221..2523)
                     /locus_tag="P5313_05490"
     CDS             complement(2221..2523)
                     /locus_tag="P5313_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY79851.1"
                     /translation="MLIARVRGTVVSTHKSPSMEGLRLLLLEKIDPVTLAGKGDFVVA
                     MDSVGANAEEVVFYVTGSSARLTEATKGRPTDATVIAVVDAIEKDGAYTYEKARSE"
     gene            complement(2533..2841)
                     /locus_tag="P5313_05495"
     CDS             complement(2533..2841)
                     /locus_tag="P5313_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004873626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY79852.1"
                     /translation="MADTTNDALGMIETRGFAAMVEAADAMAKAAKVDLVGYEKIGGG
                     YVTAVVRGDVAACRAAVDAGARAAEKVGEVVSTHVIPRPHESVEAALPLGRKPAPARK
                     "
     gene            complement(2877..3518)
                     /locus_tag="P5313_05500"
     CDS             complement(2877..3518)
                     /locus_tag="P5313_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79853.1"
                     /translation="MNIDLRTYVFLDSLQLQNASFIATVSKGYYPVGFQACCIIEIAP
                     GIEINRLTDIALKATAVTPGLQIVERAYGLLEVHSDRQGDARTAGEAVLRELGLKEED
                     RLKPRILTSQLIKNVSDHHAQLINKVRHGNMVLRGDTLYVLEVEPAGYAYYAANEAEK
                     TARVNIVEVLGFGAFGRVYISGDEAEVIEARKTVEARLQGLTGRTIGAGTQRI"
     gene            complement(3522..3818)
                     /locus_tag="P5313_05505"
     CDS             complement(3522..3818)
                     /locus_tag="P5313_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY79854.1"
                     /translation="MAGSTNDALGMIETKGFAAMVEAADTMVKAAKVDLVGYEKIGGG
                     YVTVVVRGDVAACRAAVDAGVRSAEKVGEVVSTLVIPRPHASVDEALPLGRKGD"
     gene            4058..4834
                     /locus_tag="P5313_05510"
     CDS             4058..4834
                     /locus_tag="P5313_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRY79855.1"
                     /translation="MTKGERWNAIMKRLGFETAASILELSRDLDVSPMTVRRDLAALA
                     REGKVRVLYGSAMLHPNLARREGDAHYSLNAAGAEHAEEKRRIGKAAAALAEPGDSLI
                     VDCGSTTEYVARYLPEDEEYTVLCYSLNVVSELVRRSGCRTHFSGGLFHENTLMFESP
                     EGLEAIRHFRANKAFLSASGVDARFGVTCSNAYERETKKAVIQSSLRKILVVDSSKFG
                     AVRPAYFADLADFDEIVTDPGIPDEYRRIAGERGIRLTVV"
     gene            complement(5143..5814)
                     /gene="deoC"
                     /locus_tag="P5313_05515"
     CDS             complement(5143..5814)
                     /gene="deoC"
                     /locus_tag="P5313_05515"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRY79856.1"
                     /translation="MKQWTRAELAKAIDHSLLKAFSTERMAEEHCAEARAFGFYGVCV
                     NPYWVRFCLRKLAGSGIAVCTVVGFPLGANAPESKAEEARLAVRSGAGEIDMVINVGA
                     AKSGDWKAVEEDIRGVVAASGPAAVKVILETCYLNGSEKARACEAAIRAGARYVQTST
                     GFGTGGASVDDIRILRDAIRDRDLRIKASGGIRTYHDAVQLLEAGADRIGASSGVAIV
                     SELPE"
     gene            complement(5826..6380)
                     /locus_tag="P5313_05520"
     CDS             complement(5826..6380)
                     /locus_tag="P5313_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79857.1"
                     /translation="MDSEAMIRLKEMHLAPYIQLATTLVGKRRRSGGNMFRHQIDTFG
                     ILMDYGYIDSVLLKASVIHDLLEDVPDFDRDAILSIDEESPEVYKLVLEVTRRPIESK
                     QQFLERIQRFGSKRAKVLKCADRISNIISLGYVTDMRFIKRYTDETEALIFPIAQSAD
                     KRMLEELEELVASRRENLATKFEI"
     gene            complement(6521..9205)
                     /locus_tag="P5313_05525"
     CDS             complement(6521..9205)
                     /locus_tag="P5313_05525"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRY79858.1"
                     /translation="MKAIELAKAYAPKEFEDRIYESWESAGHFKPAGEPGKKPFVIVI
                     PPPNVTGVLHMGHGLNNSLQDIAIRYNRMLGRPTLWVPGSDHAGIATQHVVEKKLRAK
                     GILRRDLGREKFVEETWKVANEHHDIIKKQLEKIGSSCDWTRERFTLDEGLSKAVREV
                     FVTLYERGLVYKGEYLVNWCTSCGTALADDEVDHADEAGGMYHIRYEIVDPPAGSPPE
                     GGWTVIGGKAYIEIATTRPETLLGDTAVAAHPEDPRYAALIGKKVKLPLTDRTIPIIA
                     DSYVDRAFGTGLVKITPAHDPNDFEVGNRHNLERVNILTPDGRLSDAAPAKYRGLAVK
                     EARKAVTADLEAAGLFVREEKISHSVGHCYRCHSVVEPYLSEQWFVRMKPLAEKALKV
                     WRDGDVRFYPQRWENTYEHWLTNIRDWCISRQLWWGHRIPAWYCAHCGKTVVAREDPL
                     ECPYCKSTELTQDEDVLDTWFSSWLWPFSTLGWPKDTADLRKYYPTSVLVTGYDIIFF
                     WVARMIMAGMEFMERSPFRDVCITTLIRDKQGRKMSKSLGNGIDPLEIVDEYGADALR
                     FTLAFMFTASQDVLIDKEDFKLGSKFANKVWNASRYILGNLEGRELRAPGTADLSPLD
                     RWIRHRLSEAAGTARAALETYRFNDGAQAVYEYFWNDFCDWYIEGTKLSFRSGDEAEK
                     DRAATAILDVLEESLRLLHPFLPFVTEEIYRMLPNRRGDLIVQRYPEADAARRDPALA
                     AEFETVMEFVRLVRNLRSEFGIEPQKAVATAVRFEAGAAAAGSFPRWVPLAALLVNGP
                     DPEVVTAKPEGSLALVGRGFEAYAKVKDAVDGAALALKFRKEAEKERGFAERVRAKLG
                     NESFLKSAPPEVVDKEREKLAEAERRAAKLEQYVKELA"
     gene            9463..11388
                     /locus_tag="P5313_05530"
     CDS             9463..11388
                     /locus_tag="P5313_05530"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HRY79859.1"
                     /translation="MRIPIRQSASMILGYARGKLGAQVRAVSFLVLYLAAFQFLVLRR
                     PIESALSVAAGIGAVAAGLALFLEGLFLGVMPLGERCGLRLPSRARGAGIVLFSLVLG
                     ITATLAEPAIGFLRAQGSAVRPWDAPMLYLLLNRGSPWLVAAIAVGVGIAVVLGVFRF
                     IRDWSLKPFLFTLVPLLLGLSLWAALDPVLAPAAGLAWDAGGVTTGPVTVPLVIALGI
                     GVSRIVGRGDGSSGGLGVVTLASALPVLTVLLLALSLGPRTPAPSDRAGFFAPEARAK
                     AEFVIGSAEGLRDLAARELGTEDYERWFDSAATPVSEDLPPSTAESSSFLRGPLTVHL
                     ADALKAILPLSFVLIFTLRLLLRERIANADEMLLGLGFAVAGLFLFGIGMERGLNILG
                     REAGRSLPQAYTSSPRPDRAETFQGIDESLVLRARGPEGTLEYLPVARPEGPEFLPFD
                     RTRFDSRAGTYVHIPVQRPILADAPEWAGYAAVLLFVFVLGFGATLAEPSLNALGSTL
                     EELTTGTYKRTFLVRTVAVGVGFGMAAGFARILLDLPLILVLGIPYLAALILTAFSSD
                     EFIGIAWDSAGVTTGPITVPLVIAAGLGIGERSAALDSFGVVAAASVFPILAVLSSGL
                     AVRLRARRALAVNLDDL"
     gene            11397..12560
                     /locus_tag="P5313_05535"
     CDS             11397..12560
                     /locus_tag="P5313_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79860.1"
                     /translation="MNKERVSKITCIAASSLGSSILSALEDSGLRDTFVQRGKQVSLA
                     DRAGMFRLVPRVELEDSAADIYRFYAPPDRAEEICARISDFTGLALPGRGSLFVEPAE
                     IVRGAPGGEATPSPESGEGPIHGKGALPDRLRLPSGLQDYTALTCIVQRGQGDALART
                     VLEMGLCVPVVTYGQGMGLRNKLGLLRITIPVDKEVIYFLVPAADGPLAENIAVHKAR
                     LDRPGQGFIYRCRVRAAAVNTRMSRSRRRHVASMEQVILAVDSLQGSTDWRRITAPSR
                     GRGGQTDEGPALACLSVICDEGRAGDFVRAAMEAGAGGATLMRLGYRDLPGAAAEHRE
                     TSPSHARETCDLIVPADLTDDILAALADRGLFEPEVYGLVEITTVEKAVTYRG"
     gene            complement(12733..13530)
                     /locus_tag="P5313_05540"
     CDS             complement(12733..13530)
                     /locus_tag="P5313_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY79861.1"
                     /translation="METKTATVKGRKILYRESGAGYPVLLVHGNTGSSLWWTRVGEVA
                     GARLTAPDLPNFGGSDRLDPAEAPGSSDIDLYADYLIAFAEDLGLFRPVLVGHSLGGA
                     VAISALVRRPELWASAVLVDSAPPGGLVTPEAHYAAIERFKTDRALLRSALGAVCPAI
                     DDPAFLDRLTDEALRMNPESFAGNARALARFDRSGDCGRYPGPVLVLWGRRDAIVTEA
                     MARDTAAAFPRGSLRILENVGHSAMVEDPAAFREILAGVVRRADPGY"
     gene            complement(13694..14494)
                     /locus_tag="P5313_05545"
     CDS             complement(13694..14494)
                     /locus_tag="P5313_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY79862.1"
                     /translation="MERIEVGGCGLAYEVTGGGPRTVALLNGIAMSVSHWKPVTERLA
                     AAGWRVLCHDMRGQLLSDKPGGYSLEGHARDLAALMDRLGIPRLHVMGTSYGAEVGLA
                     FARDFPDRVESLVLVDGVSETDALLEAAVESWMRAARAARADPGLFYRTIIPWNYSAD
                     YIAANREALDKREAAIRSLPPDYFEAFAGLCDAFLKIDLTKDLGRIRCPTLAVVAEKD
                     ILKHRGFSEILVRGVPGARLAMIPGAGHAVVVEAPDAVADAALGFWGP"
     gene            complement(14496..15200)
                     /locus_tag="P5313_05550"
     CDS             complement(14496..15200)
                     /locus_tag="P5313_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79863.1"
                     /translation="MILETKNLTCGYGKLPVLRDVNIHIGAESVALIGPNGAGKTTLI
                     NAITGLVRPWGGSVLFDGRDLAGQATHQIARAGIALVPQERELFPGMTVADNLALGAA
                     YIPRAQGQEERLLDQVIRLFPILKERYRQIVGTMSGGQQRMVAIARALMANPKLLILD
                     EPSLGLQPSIVSEVFDRLSELKKDVAILVTEQNVKESLRSVDRGYVLENGVVVLEGAA
                     ADLAKDPHVEASYLGL"
     gene            complement(15197..15916)
                     /locus_tag="P5313_05555"
     CDS             complement(15197..15916)
                     /locus_tag="P5313_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79864.1"
                     /translation="MKTLVTEHLTKRFLGLVAVNDVSFSLDAGEILGIIGPNGAGKTT
                     FIHLVSGILMPSSGKVLYKGRDVSYTPAHERARAGIARTFQLIHPLENLTVIENVMIG
                     AVFSRGQSLKEARKSAEGICESLRLGDLGRDTASLSILEIKKMEIARALANGPEVLFL
                     DEVMAGLNTDETRQMISMVKAIAKERGLAVGVVEHVMGVIRELTERVIVLESGELIAE
                     GPYSEVSKNPRVIEAYLGGAA"
     gene            complement(15913..16839)
                     /locus_tag="P5313_05560"
     CDS             complement(15913..16839)
                     /locus_tag="P5313_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY79865.1"
                     /translation="MKRNTLYLAAAAAAVLAAALPAFLGAYPLNVARSVMTYMALAVS
                     WDMLLRSGQISFGIAGLFGLGSYASALLVLRAGMDPAMSLVFATLFTGALAFTIGFAV
                     LRLRAMYFSITTLALAEIFRIVLHNLHDFSGGPEGVVLPGVIFGGDTRALYWLCLGGL
                     AITLGVSWYFEKSKLHLALTAIRNDETSARSSGVDIFRYLLIAFTITSTLQGMMGAIQ
                     VQSYGFTTPETAFDANFTLLPLAMALLGGIHSTLGPMAAAVLLGIASEWLKLKIPYGH
                     LVVYGIIIVLVILLMPRGLVGLARKRRTGRGA"
     gene            complement(16836..17756)
                     /locus_tag="P5313_05565"
     CDS             complement(16836..17756)
                     /locus_tag="P5313_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY79866.1"
                     /translation="MANKRNWTVALALGAAVIGILVWRPTVLIYGLQAAGIYASIAIP
                     MGLILGIVHIVNLAHGEFMMAASYLTYMVCRATGMDPFLALVPVAAVTAAFGFGIYKL
                     TIRQALKAPELNQLILTFGLAILLTQAANLAFTSQTRKISFDYVSASVDIGELSFGIY
                     NFVFVAIAVAMVVGLRLFLTRTTTGKAALAVGQNPRGAAIVGIDVDRTYALVFSLASA
                     LVGMMGAVFITRSSIFPAVGSPFTMKSFCLIAMAGVGNIPGILASSVLLGVSENFLKA
                     FRGTRGWADIVFFVLIITVILGRSIRGKKA"
     gene            complement(17826..19022)
                     /locus_tag="P5313_05570"
     CDS             complement(17826..19022)
                     /locus_tag="P5313_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79867.1"
                     /translation="MKRTFVALALIAAAAMAFGQGTIKIGATWPLADITGKQGSMAAQ
                     QAVDEINKAGGVLGRQLELIVIDDELKADKGAAAIEKLVTVDNVDLIIGGMSSGVALG
                     QVPAMKKYGKVVVATGAASSTVEAALGADADWYFHLHPWDYNQGASYAQGWDEIQKKY
                     PNVKIKKIFLAYEEGAFGKASFDQTKILFGAEGRYQIDGAPFKSAALGGGDYGSVLEA
                     AKMYKPDLFLWAGYDADALPMLEQAKAMKFSPGIYLGAPPGWPVGFGTSRLSENVMLY
                     GMWAPSINEVSPVSKKFYDGFVAKYKAEPATYFAPLSYSAVYIVADAIKRAGSTDTAA
                     LIKALKETKYESPLGQTVTFTPAKIIKNQGIKNQKILQWQRGRQEVIWPFESATAKPV
                     YPFPKW"
     gene            complement(19135..20154)
                     /locus_tag="P5313_05575"
     CDS             complement(19135..20154)
                     /locus_tag="P5313_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRY79868.1"
                     /translation="MPDHVGILGIGTYIPPARMSAADLAAASGLPEDVVASKFGVLQK
                     PVAGPDEPTSELGLRAAREALKDAGVPAADVDLVIWCGAQHKDYPCWLAGLSVADKLG
                     ANRAWSFDMEAMCGSMMAALDVAKSLMLARDDLRTVLLVSGYRNNDLIDPAETTTRFM
                     LDVGSGGAACVLRKNAGRNVILASAFRGDGSFSEMCVVPVLGSRAWPPRSGDADRPFF
                     KVPDEEAFKRKLGETTMPNFYAVIRDSLAKSGLSEKEIGYLAILHFKRSAHLAVLAEL
                     GLPESRTTYLEDYGHLGQNDQLLSLQLGLASGKVRDGMNVVLVGAGLGFVWAATTVRW
                     GPYPG"
     gene            20555..21652
                     /locus_tag="P5313_05580"
     CDS             20555..21652
                     /locus_tag="P5313_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY79869.1"
                     /translation="MAAAIRLFARKWFAVVSVAEICREAGVSNGLFYRYFRSKEEIFR
                     LILESVLKSIGELLAGVRGDSVEERIRSFVRSVADYSTEHRDLVSIFREGQYRYFEYE
                     KRLTGQYIDAVAGVLGRKAEIPEYLAAAAGLRFCAVRRAFHETPFDYETMEAILEAGI
                     FRDAPEPRYERIFDVVVRPLPISLEEGTRDRLIKAGKRLFGERGYHETNIHDITGSLG
                     LAVGSFYTYFGSKEAFFSEIIDRVGREIRGFISLNLDHELTRLERELQGIYLFSFFLT
                     LDPHCYPIVREAEFVLPGAVKEYYDAFQAGYEKNLGDLGALHHPTVINYLMGISHYFG
                     IEVAFDESPQNVRNVVRSLGAYLTRGARTPA"
     gene            21728..>22395
                     /locus_tag="P5313_05585"
     CDS             21728..>22395
                     /locus_tag="P5313_05585"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRY79870.1"
                     /translation="MAKARIIGTGVYAPGEPIDNRELMKLAGIEFDAAKHEEKLGIRS
                     RHIARLRGMSETTADFAEKAARAALADAGVDPKDVGLFVVGTDTPEYVSPATAILVQG
                     RLQCGETDAGAYDVGASCASFTIALDQAARVLATEPGLRYAVVAGVYDMPAHIRPGDA
                     FGWSIFADGAGAAVLERVSDDDLSGYVTGRQRTDGTQWNFIGVYAGGTRKPVTRELLE
                     AGQYG"
BASE COUNT         3450 a         7727 c         7494 g         3724 t
ORIGIN      
        1 ccgggcggag ccgccctcgt ccaccaccag cacgaggtcc ccgatcccgg cctggacccc
       61 gtccacggcc aggagggtcc tgccccggaa actcccgtcg ggatccacgg ggcggaccgc
      121 caggatcttc ttgcccgtgt agcggggatc cttctccgtg gatacgacgc tggcgacgac
      181 ccgggccagg atcacggggc gcctccccgg tccaccgcgt ccaccacgcc caggatcgcc
      241 gcgtccgccg ggttgaacca cccgttcggg ttcgcctggg cggcctcctt gcccgactcg
      301 aagatcacga ggtccccttc ccccgcccgg gccgcgtcga aggccaccag gggcggcccc
      361 gcggaggcgc ccgtctcgtc gacgggctgg accaggagga gcttgagccc ctcgaggcat
      421 tccaacttgc gggtgctgac cacccggccg atcaccttcc cgagtttcat agccagcctt
      481 tctacacgat ccggaaatag tcgaccaggg tgcagcgccg ctcccgggtg aaagacgtgg
      541 gccgggtgat cccctcgccc gtggggctcg cgatcgtgaa ggacgcgtgc cccgcgccac
      601 cctcccccag ccccgcgtag cagggcccgt tcttgacgaa gagggagcag tccatggccc
      661 gggccatgtg ggacaggcgg gccacgtcgt gggagtgcat gatcgcggtg tgccggtacc
      721 cgtgctccac aaggacggcg aactcgatgg cctcgtcggc gttccgcatg cggacgaagg
      781 gaatcaccgg catgagctgt tccgtccaga ccaggggatg tccgctcgtc gtctccagga
      841 gcaggatccg cgtgccctcg ggcacctcga tcccggccgc ccgggcgatg tgggccgggc
      901 tcttgccgac gaaggcccgg ttcggggagc ccggccggcc gggtcctccc ggctcggcca
      961 ggacgagctt ggtgaccgcg tcgatctggc cgcccttgag ctcgtaggcc ccgttccgga
     1021 ggagctcctc cttgaggcgg tccgctatgg aggcgaccgc caggatctcc ttctcgcaga
     1081 tgcacaccac gttgtggtcg aagccggccc cgtccacgat gtccttgccg gccttggcca
     1141 ggtcggcggt ctcgtccacg acgcagggcg ggttcccggg gcccgcgcag atggccttct
     1201 tgccgctggc catggcggcg cggacgaccc ccgggcctcc ggtcaccacc aggagggcca
     1261 ccccggggtg ccgcatgagc tcccccgcgg tctccagggt cggctccgcc acggcgcaca
     1321 ggaggtttcc cggacccccg gcgcggacga tcgccccgcc gagcatctct atgaggaggc
     1381 gggagacccc cttggcgccc ggatgcacgt tgaacaccac ggagttcccc gccgcgacca
     1441 tcccgatggc gttgctggcg atcgaggaga tgggattcgt caccggggtg atcgcgccga
     1501 tcaccccgta gggggccagc tccgtgaggg tgagcccgtg ctcgtccgtg accacccggg
     1561 actcgaggtc ctcgaccccc ggggtgcgga gggcggccag gcggttcttg accgtcttgt
     1621 cccggacgtt gcccaggccc gtctccgcga cggcggcctg gctcagggcc tcgttggcct
     1681 cgagcacggt ccggcggatc gcgtcgatca cggcccgccg cgtgtccagg ggaagccgga
     1741 cgagctcccg ctgggcagcc tgggccgcct ggacggcgga atccatgtcc gggaagatcc
     1801 ccggaccccg ggaggccgcg gcgctcccgc ccttcgtccc cgaggacagg agggcggcga
     1861 tctccgggga ccgcagaatc tcctcgacca cccgacgaac atcctccgcg ccgtacgcca
     1921 tctatcgccc ccgttccgac tcgttccaga gcgtcgcccc gccggagtcc accgcgtcca
     1981 cgatccccac ggccagggtg tccatgggcg cgttctcggt ccggtaggtc cagcggcagg
     2041 agctgccctg ggtcaaaagg accagctggc cccgctccgc gcccaccatg tccagggcca
     2101 ccaggtagtc cccccgcccg gctccctcgg ggccgcagtc ctccaccagg aggtaccgag
     2161 ccccggcaag gccgtcggag cggcgggacg cgaccacggt gccccggacc ctcgccaggg
     2221 tcattcggac cgcgccttct cgtaggtgta cgcgccgtcc ttctcgatcg cgtccaccac
     2281 ggcgatcacc gtcgcgtccg tggggcggcc cttggtcgct tccgtgaggc gggcgctgga
     2341 gccggtcacg tagaacacga cctcctcggc gttggccccg acggaatcca tggcgaccac
     2401 gaagtcgccc ttgcccgcga gggtcacggg atcgatcttc tcgaggagga gcagccggag
     2461 cccctccatg gaggggctct tgtgggtgga gacgacggtc ccccggaccc gggcgatcag
     2521 catgggtcgg ccctatttcc gggcgggggc cggcttcctg cccaggggca gggcggcctc
     2581 cacggattcg tggggccggg gaatcacgtg ggtcgagact acctccccca ccttctccgc
     2641 ggcccgggca cctgcgtcga cggccgcccg gcaggcggcc acgtcccctc gcacgacggc
     2701 ggtgacgtag ccgccgccga tcttctcgta cccgaccagg tcgaccttcg ccgccttggc
     2761 catcgcgtcc gcggcctcca ccatcgccgc gaagccccgc gtctcgatca tgccgagcgc
     2821 gtcgttcgtt gtatccgcca tatccgctcc ttagaagggc gtcccggaag gggcgctcag
     2881 attctctggg ttccggcccc gatcgtccgg ccggtcaggc cctgcagccg ggcctccacg
     2941 gtcttccggg cctcgatcac ctcggcctcg tccccggaaa tgtacacgcg cccgaaggcc
     3001 ccgaagccca ggacctccac gatgttcacc cgggcggtct tctcggcctc gttggccgcg
     3061 tagtaggcgt agcccgcggg ctccacctcc agcacgtaca gggtgtcccc ccgcaggacc
     3121 atgttcccgt ggcggacctt gttgatgagc tgggcgtggt ggtccgagac gttcttgatg
     3181 agctggctcg tgaggatccg gggcttgagc cggtcctcct ccttgaggcc gagctcccgg
     3241 agcacggcct ccccggcggt gcgggcgtcc ccctggcggt cggaatggac ctccaaaagg
     3301 ccgtaggcgc gctccacgat ctggagcccc ggggtcacgg cggtcgcctt gagggcgatg
     3361 tccgtgaggc ggttgatctc gatgcccggg gcgatctcga tgatgcagca ggcctggaat
     3421 ccgaccgggt agtagccctt gctgacggtg gcgatgaagg aggcgttctg gagctggagg
     3481 gaatccagga acacgtaggt ccgcaggtcg atgttcatgg atcagtcccc cttccggccc
     3541 aggggcaggg cctcgtccac ggaggcgtgg ggccggggga tcacgagggt cgagacgacc
     3601 tccccgacct tctccgccga gcggactccc gcgtccacgg cggcccggca ggccgcgacg
     3661 tccccgcgga cgacgaccgt cacgtacccg ccgccgatct tctcgtatcc caccaggtcg
     3721 accttcgcgg ccttgaccat cgtgtccgcg gcctccacca tggccgcgaa tcctttcgtc
     3781 tctatcatgc cgagagcgtc gttcgtcgat ccagccatac ttcctcctgg cgcgccgcgg
     3841 cggcccggga cggccgtgcc gccctccggg aaacccggcc gacttggctc aagtatagag
     3901 gacggaatgt tgcaggtcaa gcaatcttcc ggaatttatg gtaattttac aacattccat
     3961 ggtatagttt taccatcagg accgacgaca ttaccgtccg ggccgagccg gacaccggcc
     4021 gtcccccggg cggaccgagg acgagtggag taagggcatg acgaagggcg aacgctggaa
     4081 cgcgatcatg aagcggctcg gcttcgagac ggcggcgagc atcctggagc tctcccggga
     4141 cctcgacgtg tccccgatga ccgtgcgcag ggacctggcc gccctggccc gggagggcaa
     4201 ggtccgcgtc ctgtacggaa gcgccatgct ccacccgaac ctcgcacgga gggagggcga
     4261 cgcgcactac tcgttgaacg cggcgggagc cgagcacgcg gaggagaagc ggaggatcgg
     4321 gaaggccgcc gcggccctcg cggagccggg ggacagcctc atcgtcgact gcggctccac
     4381 gacggaatac gtggcccggt acctgcccga ggacgaggaa tacaccgtcc tgtgctactc
     4441 cctgaacgtc gtgtccgagc tcgtgcgccg gagcggctgc cgcacccatt tctccggggg
     4501 cctgttccac gagaacaccc tgatgttcga aagccccgag ggcctcgagg caatacggca
     4561 tttccgggcc aacaaggcct tcctctccgc ctccggggtg gacgcccggt tcggggtcac
     4621 ctgcagcaac gcctacgaga gggagaccaa gaaggccgtg atccagtcct ccctccgcaa
     4681 gatcctggtc gtggattcct ccaagttcgg cgccgtgcgt ccggcctact tcgcggacct
     4741 cgcagatttc gacgagatcg tgacggatcc cgggatcccg gacgaatacc gcagaatagc
     4801 cggggaacgg ggcatccggc tgactgtcgt ctagcaggtt gtagaaatgc ttctagcatt
     4861 tctacaacct gctctgcaat tgcgccgggc cctcggcccg gcggccacga agcgcttcgc
     4921 gcttcgtcga atttcacgta ccgacggggc gccgggccgc gggcccggcg gcgccgaagc
     4981 caaggcttcg gcgattttcc gcaggactac tccggcaact ccgatacgat cgcgacgccg
     5041 gaacttgctc cgatccggcg cggggcgcgg cgcgccccgc ggccacgaag cgcttcgcgc
     5101 ttcgtcgatc ttcacgaacg gtgcggaccg gatacgggca tgctattcgg ggagttccga
     5161 gactatggcg acgccggaac ttgcgcctat ccggtccgcg ccggcctcca ggagctgaac
     5221 ggcgtcgtgg taggtccgga tgccgccgga ggccttgatg cggagatccc ggtccctgat
     5281 cgcgtcccgt aggatccgga tgtcgtccac gctcgcgccc ccggtcccga agcccgtgct
     5341 cgtctggaca tagcgggcgc ccgcccggat cgccgcctcg caggcgcggg ccttctcgga
     5401 cccgttcagg tagcaggttt ccaggatgac cttcaccgcc gcggggccgg acgcggccac
     5461 gaccccccgg atgtcctcct ccacggcctt ccagtccccg gacttggcgg cgccgacgtt
     5521 gatgaccatg tcgatctccc cggcccccga ccggacggcg agccgggctt cctccgcctt
     5581 ggattccggg gcgttcgccc ccagggggaa gccgactacg gtgcagaccg cgatcccgct
     5641 gcccgcgagc ttccgaaggc agaagcgcac ccagtaggga ttcacgcaga ctccgtagaa
     5701 cccgaacgca cgagcctcgg cgcaatgctc ctccgccatc ctctccgtgc tgaacgcctt
     5761 gaggaggctg tgatcgatcg ccttggccag ctcggcccgg gtccactgtt tcatgccgca
     5821 acccctcata tctcgaactt cgtcgccagg ttttcccggc gggaggctac cagttcctcg
     5881 agctcctcca gcatgcgttt gtccgcggac tgggcgatcg gaaagatcag ggcctccgtc
     5941 tcgtcggtgt atcgtttgat gaagcgcatg tccgtcacgt accccaggga gataatgttg
     6001 ctgatccgat ccgcgcattt caggaccttg gcgcgcttgg atccgaagcg ctggatccgt
     6061 tccaggaact gctgcttgga ttcgatgggc cggcgggtca cttccaggac cagcttgtag
     6121 acttccgggc tctcctcgtc gatggaaagg atggcgtccc ggtcgaagtc gggcacgtcc
     6181 tccaggaggt cgtggatcac cgaagccttg aggaggaccg agtcgatgta cccgtagtcc
     6241 atcaggatgc cgaaggtgtc gatctggtgg cggaacatgt tgcccccgct ccgcctccgc
     6301 ttgcccacca gggtggtggc cagctggatg tagggggcca agtgcatctc cttgaggcgg
     6361 atcatcgcct cggagtccat gagcccctcg tcttccaagc cagggtggaa cggggccagg
     6421 ggcccgggat cgaagccccc ggtcgtcggc gagtccgggg ctgtggattc ctccgggacg
     6481 acgggcgcct tcacgctccc accctcccgg ggcttcccgt tcatgcaagc tccttcacgt
     6541 actgctccag tttggccgcc cggcgttccg cctcggccag cttttccctt tccttatcta
     6601 cgacctccgg aggcgcggac ttgaggaagg attcgttgcc gagcttggca cggacgcgtt
     6661 cggcgaagcc ccgttccttt tccgcttcct tgcggaactt cagggccagg gccgcgccgt
     6721 ccaccgcgtc cttcaccttc gcgtaggctt cgaagccgcg gcccaccagg gccagggatc
     6781 cctcgggctt ggcggtcacg acctcgggat ccggcccgtt caccaggagg gccgccaggg
     6841 ggacccagcg ggggaaggat ccggccgcgg ccgccccggc ctcgaagcgt acggcggtcg
     6901 cgacggcctt ctggggctcg atcccgaact cgctgcggag gttgcgcacc aggcgcacga
     6961 actccatcac ggtctcgaac tccgcggcca gggcgggatc ccggcgggcg gcgtccgcct
     7021 cgggataccg ctggacgatc aggtcccctc gccggttggg gagcatccgg tagatctcct
     7081 cggtcacgaa gggcaggaag ggatggagga gccggaggct ctcctccagg acgtccagga
     7141 tggccgtggc cgcccggtcc ttctccgcct cgtccccgga gcggaaggaa agcttggtcc
     7201 cctcgatgta ccagtcgcag aagtcgttcc agaagtactc gtacaccgcc tgggccccgt
     7261 cgttgaagcg gtaggtctcc agggcggccc gggcggtacc ggcggcctcg gaaaggcggt
     7321 gccggatcca gcggtccagg ggcgacaggt ccgccgtgcc cggggcccgg agctcccggc
     7381 cctccaggtt gcccaggatg taccgggagg cgttccagac cttgttggcg aacttggagc
     7441 ccagcttgaa gtcctccttg tcgatgagga cgtcctggga ggcggtgaac atgaaggcca
     7501 aggtgaagcg gagggcgtcc gccccgtact cgtccacgat ctccagggga tcgatcccgt
     7561 tccccaagga cttggacatc ttccggccct gcttgtcccg gatcagggtg gtgatgcaca
     7621 cgtcccggaa gggggacctc tccatgaact ccatgcccgc catgatcatc cgggccaccc
     7681 agaagaagat gatgtcgtac ccggtcacca ggacgctggt ggggtagtac ttccggaggt
     7741 ccgcggtgtc cttgggccag cccagggtgc tgaagggcca gagccaggag gagaaccagg
     7801 tgtccaggac gtcctcgtcc tgggtcagct ccgtggactt gcagtacggg cactccaggg
     7861 gatcctcccg ggccaccacg gtcttgccgc agtgggcgca gtaccaggcg gggatccggt
     7921 gcccccacca gagctggcgg ctgatgcacc agtcccggat gttcgtgagc cagtgctcgt
     7981 aggtgttctc ccagcgctgg gggtagaacc ggacgtctcc gtcccgccag accttgaggg
     8041 ccttctcggc caggggcttc atccgcacga accactgctc cgagaggtag ggctccacga
     8101 cggagtggca gcggtagcag tggcccacgg agtggctgat cttctcctcc cggacgaaaa
     8161 ggcccgcggc ctcgaggtcc gccgtcaccg ccttccgggc ctccttgacg gccaggcccc
     8221 ggtacttggc cggagccgcg tcggagagcc tgccgtccgg ggtcaggatg ttcacccgct
     8281 ccaggttgtg gcggttgccc acctcgaagt cgttggggtc gtgggcgggg gtgatcttca
     8341 cgaggcccgt cccgaacgca cggtccacgt aggagtccgc gatgatcggg atcgtacggt
     8401 ccgtcagggg aagcttgacc ttcttcccga tcagggccgc gtagcgcggg tcctccgggt
     8461 gggccgcgac ggcggtgtcc cccaggaggg tctccggccg ggtcgtggct atctcgatgt
     8521 aggccttccc gccgatcacc gtccacccgc cctccggagg ggagccggcc gggggatcga
     8581 cgatctcgta gcggatgtgg tacatccccc ccgcctcgtc cgcgtggtcc acctcgtcgt
     8641 ccgccagggc ggtgccgcag gaggtgcacc agttcaccag gtactcgccc ttgtacacga
     8701 gtccccgttc gtagagggtg acgaagacct cccggacggc cttggaaagg ccctcgtcca
     8761 gggtgaagcg ctcccgggtc cagtcgcagg aggagccgat cttctccagt tgcttcttga
     8821 tgatgtcgtg gtgttcgttg gcgaccttcc aggtctcttc cacgaacttc tcccggccca
     8881 ggtcccggcg gaggatcccc ttggcccgga gtttcttctc caccacgtgc tgggtggcga
     8941 tgcccgcatg gtcgctgccg ggcacccaga gggtcgggcg tccgagcatc cggttgtacc
     9001 ggatggcgat gtcctggagg gagttgttga ggccgtgccc catgtggagg acccccgtaa
     9061 cgttcggggg aggaatgacg atgacgaagg gtttctttcc gggctctccc gcgggcttga
     9121 agtggccggc ggactcccag gactcgtaga tgcggtcctc gaattccttg ggggcgtagg
     9181 ctttcgcgag ttcgatggct ttcatgggcg ttcccttcgt cgctgcgttc tcccgcgctc
     9241 cccgaggagc ggcgcggacc gcgaataggg agaaactcta ccaaaatgcg gggataattt
     9301 caaaggggag gtgaaaaaac cgaaaactcg catccgaacc ggacggccgc ggcgggttcc
     9361 cttcactcgg ggcccctccg cgtatagaat gaaggtagac caagaatccg gagagttccg
     9421 atgcctcgat cgtccttcgg ctccgcgtcc cggaaaggcc aagtgcgcat tccgatccga
     9481 caaagcgcct ccatgatcct gggctacgcc cggggaaagc tgggcgccca ggtccgggcc
     9541 gtatcgttcc tggtcctcta cctggccgcc ttccagttcc tcgtcctgcg ccggcccatc
     9601 gaaagcgcac tttcggtggc cgccgggatc ggggccgtgg ccgcggggct ggccctcttc
     9661 ctggagggac tcttcctggg ggtcatgccc ctgggggagc gctgcggtct ccgcctgccc
     9721 tcccgggccc ggggggcggg gatcgtcctc ttttccctgg tcctgggcat caccgcgacc
     9781 ctggcggaac ccgccatcgg cttcctccgg gcccagggta gcgccgtccg accctgggac
     9841 gcgccgatgc tctacctcct tctgaaccgg ggctccccct ggctcgtggc ggccatcgcc
     9901 gtgggagtgg gtatcgcggt cgtgctgggg gtgttccggt tcatccggga ctggtccctg
     9961 aagcccttcc tgttcaccct cgttcccctc ctcctggggc tgtccctctg ggccgccctg
    10021 gacccggtcc tggcccccgc ggcggggctg gcctgggatg ccggaggggt gaccaccgga
    10081 cccgtgacgg tccccctcgt gatcgccctg ggcatcgggg tgtcccggat cgtgggccgc
    10141 ggggacggat cctccggggg attgggggtc gtgaccctgg cctccgccct gccggtcctg
    10201 acggtcctcc tgctggccct gtcgctggga ccccgcaccc cggccccctc ggaccgggcg
    10261 ggtttcttcg cccccgaggc ccgcgccaag gcggagttcg ttatcggctc cgcggagggc
    10321 ctgcgcgacc tggcggcccg ggagctgggg accgaggact acgaacgctg gttcgattcg
    10381 gcggcgactc cggtatccga ggaccttccg ccctccacgg ccgaatcttc ctccttcctg
    10441 cggggccccc tgaccgtcca cctcgcggac gccctcaaag ccatcctccc cctgtccttc
    10501 gtgctgatct tcaccctgcg gctcctgctt cgggaacgca tcgccaacgc cgacgagatg
    10561 ctcctgggcc tcggcttcgc ggtggcgggg ctcttcctgt tcggcatcgg catggagcgg
    10621 gggctcaaca tcctgggccg ggaggcgggc agatccctcc cccaggccta tacctcctcc
    10681 ccccgacccg accgggcgga gactttccag ggaatcgacg agtccctggt cctccgggcc
    10741 cggggacccg agggcaccct ggaataccta cccgtcgcga ggccggaggg accggagttc
    10801 cttcccttcg accggacccg cttcgattcc cgggcgggaa cctatgtcca catccccgtc
    10861 cagcgcccca tcctggcgga cgcccccgaa tgggcgggat acgccgcggt gctgctcttc
    10921 gtgttcgtcc tcggtttcgg ggccaccctc gccgagccat ccctgaacgc cctgggctcc
    10981 accctggagg agctgaccac ggggacctac aagcggacct tcctggtgcg aaccgtggcc
    11041 gtgggggtgg gcttcgggat ggcggcggga ttcgcgcgca tcctcctgga cctgcccctg
    11101 atcctggtgc tgggtatccc ctacctggcg gccctgatcc tcacggcctt ctcgtccgac
    11161 gagttcatcg gcatcgcctg ggactccgcg ggggtcacca cggggcccat caccgtcccc
    11221 ttggtcatcg cggcgggcct ggggatcggc gagcgatccg ccgccctgga cagcttcggc
    11281 gtcgtggccg ccgcctcggt cttcccgatc ctggcggtcc tgtcgtccgg tctggcggta
    11341 cggctcaggg cgcggcgggc ccttgccgtg aatttggacg acctgtgagg cgggacatga
    11401 ataaggaacg cgtatccaag atcacctgca tcgcggcatc ctccctggga tcttccatcc
    11461 tttcggccct ggaggactcg ggactccggg acaccttcgt ccagcggggc aagcaggtct
    11521 ccctggcgga ccgcgccggg atgttccggt tggttccccg ggtggagctg gaggattcgg
    11581 cggcggacat ctaccgcttc tacgcgccgc cggatcgggc cgaggagatc tgcgcccgga
    11641 tatcggactt cacgggcctc gccctcccgg gccgggggag cctcttcgtg gagcccgcgg
    11701 agatcgtgcg gggagccccg ggcggggaag ctactccaag cccggaatcg ggcgagggac
    11761 cgattcacgg gaagggtgcg ctcccggacc ggctcagact cccttccggg ctgcaggatt
    11821 acacggccct gacctgcatc gtccaacggg gacaggggga cgcactggct cgtaccgtgc
    11881 tcgagatggg gctctgcgtg cccgtggtga cctacggcca gggcatggga ctgcggaaca
    11941 agctgggact gctccggatc acgatccccg tggacaagga ggtcatctac ttcctggtgc
    12001 ccgccgcgga cggccccctg gcggagaaca tagccgtgca caaggcccgg ctggaccggc
    12061 cggggcaggg cttcatctat cgctgccggg tccgggccgc ggcggtcaac acgaggatga
    12121 gccgctcccg caggcgccac gtcgcctcca tggaacaggt catcctggcc gtggattccc
    12181 tccagggctc cacggactgg aggaggatca cggctccctc ccgcggccgg ggcgggcaga
    12241 ccgacgaagg tccggccctg gcctgcctct cggtgatctg cgacgaggga cgggccgggg
    12301 atttcgtgcg ggcggccatg gaggcgggag ccggcggcgc caccctcatg cgcctgggct
    12361 accgggacct cccgggcgcg gccgcggaac atcgggaaac ctcgccctcc cacgcccggg
    12421 agacctgcga cctgatcgta cccgccgacc tcaccgacga catcctggcg gccctggcgg
    12481 accggggtct cttcgagccg gaggtctacg ggctggtgga gatcacgacg gtggagaagg
    12541 cggtcacgta tcgaggatag acgctgcgcg tctatcctcg atacggcaac gaagtgcgga
    12601 gcgcttcgtc gatgacctct cgctgcgcgt ctatcctcga tacggcaatg aagcgctgac
    12661 gcgcttcgtc gataacccct acgcttcgcg ttcttcctcg atacgagaga cggcaacgaa
    12721 gcgctgacgc gctcagtatc ccgggtccgc cctccgcacc accccggcca ggatctcccg
    12781 gaaggccgcg ggatcctcga ccatggcgga gtggccgacg ttctcgagga tcctcaggga
    12841 acccctcggg aaggccgcgg ccgtgtcccg ggccatggcc tccgtgacga tggcgtccct
    12901 ccggccccag aggaccagga ccgggccggg gtaccggccg cagtccccgg agcggtcgaa
    12961 ccgggccagg gcccgggcgt tcccggcaaa ggattcgggg ttcatgcgca gggcctcgtc
    13021 ggtcaggcga tccaggaagg ccgggtcgtc tatcgcgggg cagaccgctc ccagggcgga
    13081 gcgaaggaga gcccggtccg tcttgaagcg ctcgatggcc gcgtagtggg cctccggggt
    13141 caccaggccc ccgggcggag cggagtccac caggaccgcg gaagcccaga gttccggccg
    13201 acggaccagg gcggagatcg ccacagcccc gcccagggag tgcccgacca ggacgggccg
    13261 gaacaggccc aggtcctcgg cgaaggcgat gaggtagtcc gcgtagaggt cgatgtccga
    13321 ggagcccggg gcctccgccg ggtcaagcct gtcggaaccc ccgaagttcg ggaggtccgg
    13381 ggccgtcagg cgggctccgg ccacttcacc cacccgggtc caccagaggg aggaccccgt
    13441 gttcccgtgc accaggagga ccggataccc cgcgccgctc tcgcggtaga ggatcttcct
    13501 tcccttgacc gtcgctgtct tggtttccat gcgtgactcc ttcccgcggt ccggtccgcg
    13561 gcgggcgggg caggcggccg tgcaggtcag cgccagagcc gcggcgaggg cgatcgcgag
    13621 gtcgagcgaa accgcgggcc gggccgacgg ccgccccgcg gtccggcccc tcggcttcga
    13681 cacggccgcg ggctcaaggc ccccagaatc ccagagccgc gtccgccacg gcatcggggg
    13741 cctccacgac caccgcgtgg ccggccccgg ggatcatcgc cagccgagcc cccgggacgc
    13801 cccggaccag gatctccgaa aagccccggt gcttgaggat gtccttttcc gccaccaccg
    13861 ccagggtcgg acaccggatc cggcccaggt ccttggtcag gtcgatcttc aggaaggcat
    13921 cgcagagccc cgcgaaggcc tcgaagtagt ccggcggcag ggaccggatc gcggcctccc
    13981 gcttgtccag ggcttcccgg ttggccgcga tgtagtccgc cgaataattc caggggatga
    14041 tggtccggta gaagaggccc ggatccgccc gggccgcccg ggccgccctc atccaggact
    14101 ccacggccgc ttcgaggagg gcgtcggtct cgctgacccc gtccaccagg accaggctct
    14161 cgacccggtc cgggaagtcc cgtgcgaagg cgagtcccac ctcggccccg taggaggtgc
    14221 ccataacgtg gagcctcggg atccccagac ggtccatcag ggccgccagg tcccgggcgt
    14281 ggccctccag ggagtagccc ccgggcttgt ccgagaggag ctggccccgc atgtcgtggc
    14341 aaaggacccg ccagcccgcg gcggccaggc gctcggtgac cggcttccag tgggagaccg
    14401 acatggcgat cccgttcaac agggcgaccg tcctcggtcc ccctcccgtc acctcgtacg
    14461 ccaggccgca gcccccgacc tcgattcgtt ccatctcaca accccaggta ggaagcctcg
    14521 acgtggggat ccttggccag gtcggcggcc gccccttcca ggaccacgac cccgttctcc
    14581 aggacgtagc cccggtccac ggaacggagg ctctccttga cgttctgctc ggtgaccagg
    14641 atggccacgt ccttcttgag ctccgaaagg cggtcgaaga cctcggacac gatggagggc
    14701 tggagcccca gggagggctc gtccaggatc aggagcttcg ggttggccat cagggcccgg
    14761 gcaatggcca ccatcctctg ctggcccccg ctcatggtgc cgacgatctg ccggtagcgc
    14821 tccttcaata tcgggaagag ccgaatcacc tggtccagga ggcgctcctc ctggccctgg
    14881 gcccggggta tgtaagccgc gccgagggca aggttgtccg ccacggtcat ccccgggaac
    14941 agctcccgct cctgggggac cagggctatg cccgcccggg cgatctggtg ggtggcctgg
    15001 cccgccagat ccctgccgtc gaagaggacg gatccccccc agggccggac aaggcccgtg
    15061 atcgcgttga tcagggtggt cttgcctgcc ccgttcggtc cgatcagggc cacggactcg
    15121 gcgcctatgt ggatgttcac gtcccgcagg accgggagct tgccgtaccc gcaggtgagg
    15181 ttcttcgttt ccaggatcat gcggcgcctc ccaggtaggc ctcgatgacc ctcgggttct
    15241 tggacacctc ggagtacggg ccctcggcga tcagctctcc ggactccagg acgatgaccc
    15301 gttcggtcag ctcccggatc acccccatca cgtgctccac cacccccacg gccaggcccc
    15361 gctccttggc gatggccttg accatggaga tcatctgacg ggtctcgtcc gtgttgagcc
    15421 ccgccattac ctcgtccagg aagaggacct cgggcccgtt ggccagggcc cgggcgatct
    15481 ccatcttctt gatctccagg atggaaaggc tggcggtgtc ccgtcccagg tcccccagcc
    15541 ggagggattc gcagatgccc tccgcggatt tccgggcttc cttcagggac tgcccccggc
    15601 tgaagaccgc gccgatcatg acgttctcaa tcaccgtcag gttttccagg gggtggatca
    15661 gctggaaggt ccgggctatg cccgcccggg cccgttcgtg ggcgggggtg tagcttacat
    15721 cccttccctt gtagaggacc ttcccggagc tcggcatgag gatcccggac accaggtgga
    15781 tgaaggtggt cttgccggcc ccgttggggc cgatgatccc caggatctcc cccgcgtcca
    15841 gggaaaagct tacgtcgttc acggccacga ggcccaggaa gcgcttggtc aggtgttcgg
    15901 tcacaagggt tttcatgcgc ccctcccggt ccggcgtttc cgcgccagcc ccacgagtcc
    15961 ccggggcatg agcaggatca ccaggacgat gatgatcccg tagaccacca ggtggccgta
    16021 ggggatcttg agcttgagcc actccgaggc gatgcccagg agcacggccg ccgccatggg
    16081 gcccagggtg ctgtggatcc cgcccagcag ggccatggcc aggggcagga gggtgaagtt
    16141 cgcgtcgaag gccgtctccg gggtggtgaa gccgtaggac tggacctgga tggcccccat
    16201 catgccctgg agggtcgagg tgatcgtgaa ggcgataagg aggtagcgga agatgtccac
    16261 cccgctggaa cgggcgctgg tctcgtcgtt ccggatggcc gtcagggcca gatggagctt
    16321 gctcttctcg aagtaccagg aaacccccag ggtgatggcc agcccgccca ggcagagcca
    16381 gtagagggcg cgggtgtcgc ccccgaagat cacccccggg agcacgaccc cctcgggccc
    16441 tccggaaaag tcgtgcaggt tatggaggac gatccggaag atctccgcca gggccagggt
    16501 ggtgatggag aagtacatgg cccgcaggcg caggaccgcg aagccgatcg tgaaggccag
    16561 ggctcccgta aaaagcgtcg cgaagaccag gctcatcgcc ggatccatcc cggcccggag
    16621 caccaggagg gccgaggcgt aggaccccag cccgaagagc ccggctatgc cgaaggagat
    16681 ctgcccggac cggaggagca tgtcccagga gaccgccagg gccatatagg tcatgacgct
    16741 ccgggccacg ttcagggggt aggcgcccag gaaggcgggg agggcggccg ccaggaccgc
    16801 ggcggccgcg gccgccaggt acagggtgtt cctcttcatg cctttttccc ccggatcgag
    16861 cgccccagga tcaccgtgat gatcaggacg aagaagacga tgtccgccca gccgcgggtc
    16921 ccgcggaaag ccttgaggaa gttctcggaa accccgagca ggacgctgga ggccaggatc
    16981 ccggggatgt tccccacccc cgccatggcg atcaggcaga agctcttcat ggtgaaggga
    17041 ctgcccaccg ccgggaagat cgaggaccgg gtgatgaaca ccgcccccat catgcccacc
    17101 agcgcggagg ccagggagaa gaccagggcg taggtccggt ccacgtcgat gcccacgatg
    17161 gcggcccccc gggggttctg cccgaccgcc agagccgcct tcccggtggt cgtccgggtc
    17221 aggaagagcc ggaggcccac gaccatggcc accgcgatgg cgacgaacac gaagttgtag
    17281 attccgaagg agagctcccc gatgtccacg gaggcggaga cgtagtcgaa agagatcttc
    17341 cgggtctggg aggtgaaggc caggttggcg gcctgggtca gcaggatggc caggccgaag
    17401 gtcaggatca gctggttgag ctccggggcc ttgagggcct gccggatggt cagcttgtag
    17461 atcccgaagc cgaaggccgc ggtgaccgcc gccaccggca ccagggccag gaagggatcc
    17521 atccccgtcg ccctgcagac catgtaggtc aggtaggagg ccgccatcat gaactctccg
    17581 tgggccaggt tcacgatgtg cacgatcccc aggatcaggc ccatggggat cgcgatggag
    17641 gcgtagatcc ccgccgcctg gagcccgtag atgagcacgg tcgggcgcca gaccaggata
    17701 ccgatcaccg ccgcccccag ggccagcgcg accgtccaat tccgtttgtt cgccatcggg
    17761 ttcccttttc aagaaaatac gccgccggcc cgggggccgg cggcacggtc agggtcgggc
    17821 ccggatcacc acttggggaa gggatacacg ggcttggcgg tggcggactc gaagggccag
    17881 atcacctcct ggcgtccccg ctgccactgc aggatcttct ggttcttgat cccctggttc
    17941 ttgatgatct tggcgggggt gaaggtgacg gtctgtccca ggggggactc atacttggtc
    18001 tccttcaggg ccttgatcag ggcggcggtg tccgtggacc cggcgcgctt gatcgcgtcc
    18061 gccacgatgt agaccgcgga gtaggacagg ggggcgaagt aggtggccgg ctcggccttg
    18121 tacttggcca cgaagccgtc gtagaacttc ttgctcaccg ggctgacctc gttgatcgag
    18181 ggggcccaca tgccgtagag catgacgttc tcggagagcc gggaggttcc gaatccgacg
    18241 ggccatcccg ggggcgctcc gaggtagatg ccgggggaga acttcatggc cttggcctgt
    18301 tccaacatcg gcagggcatc cgcatcgtac ccggcccaca ggaagaggtc gggcttgtac
    18361 atcttggccg cttccaggac cgatccgtag tccccgccgc ccagggccgc gctcttgaag
    18421 ggggcgccgt cgatctggta ccggccttcg gcgccgaaga ggatcttggt ctggtcgaag
    18481 gacgccttgc cgaaggctcc ctcctcgtag gcgaggaaga tcttcttgat cttcacgttc
    18541 ggatacttct tctggatctc gtcccagccc tgggcatagg acgctccctg gttgtagtcc
    18601 caggggtgga ggtggaagta ccagtccgcg tcggcgccca gggcggcttc cacggtggag
    18661 gaggcggcgc cggtggcgac cacgacctta ccgtacttct tcatcgccgg aacctggccc
    18721 agggccacgc cggaggacat gccgccgatg atcaggtcca cgttatccac ggtgaccagc
    18781 ttctcgatgg ccgcggctcc cttgtcggcc ttgagctcgt cgtcgatgac gatcagctcc
    18841 agctggcgcc ccaggacgcc gccggccttg ttgatctcgt ccaccgcctg ctgggcggcc
    18901 atgctgccct gcttgccggt gatgtccgcg aggggccagg tggcgccgat cttgatcgtg
    18961 ccctggccga acgccatcgc cgccgccgcg atcagggcga gggcgacaaa agtccgtttc
    19021 atcatgagcc tccttgaccg gcgcccttcg gggccggcgt ttcggaacaa gctatgtcga
    19081 cgcggcgccg acggcgccgc gcggatcccc gtactcgttt cgacctcgtc ccggtcagcc
    19141 gggatagggc ccccaccgca ccgtggtcgc ggcccacacg aagcccagcc cagccccgac
    19201 cagcaccacg ttcatcccgt cccggacctt cccggaggca aggcccagct ggagggacag
    19261 gagctggtcg ttctgcccca ggtggccgta gtcctccagg taggtggtcc gggactccgg
    19321 aaggcccagc tccgccagga ccgccagatg cgcgctgcgc ttgaagtgga ggatggccag
    19381 gtagccgatc tccttctcgg aaagcccgct cttggccagg gagtcccgga tgaccgcgta
    19441 gaagttcggc atcgtcgtct cccccagctt cctcttgaag gcctcctcgt cggggacctt
    19501 gaagaagggc cggtccgcgt ccccgctcct cgggggccag gccctggaac ccagcacggg
    19561 caccacgcac atctcggaaa aggagccgtc cccccggaag gcggaggcta aaatgacgtt
    19621 gcggccggcg ttcttccgga gcacgcaggc ggcgcccccg gagcctacgt ccagcatgaa
    19681 ccgggtcgtg gtctccgcgg gatcgatcag atcgttgttg cggtaccccg agaccaggag
    19741 gaccgtgcgc aggtcgtccc gggccagcat cagggacttg gccacgtcca gggccgccat
    19801 catggacccg cacatggcct ccatgtcgaa gctccaggct cggttcgccc ccaatttgtc
    19861 ggccaccgag aggcccgcca gccagcaggg gtagtccttg tgctgggctc cgcaccagat
    19921 caccaggtcc acgtccgccg cgggtacccc cgcgtccttg agggcctccc gggcggcccg
    19981 gaggcccagt tcgctggtgg gttcgtcggg gcccgccacg ggtttctgga gcaccccgaa
    20041 cttggaggcc accacgtcct cgggcaggcc cgaagccgca gccaggtccg ccgcggacat
    20101 ccgtgcgggg ggtatatagg ttccgatgcc caaaattccg acatgatccg gcataaggta
    20161 tgaatcacct ctcacttccg tagtacaccc gtttcccgga tccgtcaaga agaaaacgct
    20221 ccctgacctt caggaatgaa ccacggaggt cacggagagc acggagaaaa aggaaaagag
    20281 agacagaata ccaggatgta ccaggatcaa caggataaga tatgggaaga atatgcccac
    20341 ttccctacgt ctcatcctgt tcatccgttc ttaatcctgt tatcctgtct ctcttcttgg
    20401 attgcagcct tcctccgtgt gctccgtggt gagtccgggt cagatttttt ctcgcttgcg
    20461 ccctttccgg gcagggtgta tactcggttt cgagatgaca tctgaatcgg ttcttactaa
    20521 aatctcgaat ccaaccacta cccgggaagc cctcgtggcc gcagccatcc gcctgttcgc
    20581 ccgcaagtgg ttcgccgtcg tctcggtcgc ggagatctgc cgggaggccg gggtttccaa
    20641 cggcctcttc tatcgctact tccgcagcaa ggaagaaatc ttcaggctga tcctggaatc
    20701 cgttctcaaa tccatcggcg agctcctggc cggggtccgc ggcgattccg tggaagaacg
    20761 gatccgctcc ttcgtccggt ccgtggccga ctattccacc gaacaccggg accttgtctc
    20821 catcttccgg gaaggacagt atcggtactt cgagtacgag aagcgcctga ccggccagta
    20881 catcgacgcg gtcgcggggg tcctggggcg gaaggccgag atccccgaat acctggccgc
    20941 cgcggcggga ctccggttct gcgcggttcg acgggccttc cacgaaaccc ccttcgacta
    21001 cgagaccatg gaagccatcc tggaggccgg catcttccgg gacgccccgg aaccccggta
    21061 cgagcgaatc ttcgacgtgg tcgtgcgccc cctgccgatc tccctggagg aaggcacccg
    21121 ggaccgcctg atcaaggccg gcaagcggct cttcggggag aggggctacc acgagaccaa
    21181 cattcacgac atcaccggga gcctcggcct ggccgtgggc tccttctaca cctacttcgg
    21241 gagcaaggaa gccttcttct ccgagatcat cgaccgggtg gggcgggaga tccggggctt
    21301 catctccctg aacctggacc acgagctcac ccggctggag cgggaactcc agggcatcta
    21361 cctcttctcc ttcttcttga ccctcgatcc ccactgctac cccatcgtcc gggaagccga
    21421 gttcgtcctg cccggggcgg tcaaggagta ctacgacgcc ttccaggccg ggtacgagaa
    21481 aaacctcggg gatctggggg ctctgcacca tcccacggtc atcaactacc tgatggggat
    21541 ctcccactat ttcggcatcg aggtggcgtt cgacgaatcc ccccagaacg tgcgcaacgt
    21601 cgtgcgctcc ctgggggcgt acctgacccg gggcgcgcgg accccggctt gaggccggag
    21661 gctccgcggg gacttcacgg cgccggacgg cggtttccgc cggcgggctt atgaccagga
    21721 ggcggatatg gctaaagcca ggatcatcgg cacgggcgtc tacgcccccg gcgagcccat
    21781 agacaacagg gagctcatga agctggcggg gatcgagttc gacgcggcca agcacgagga
    21841 gaagctcggc atccgctcaa ggcacatcgc ccgcctgcgg ggcatgtcgg agaccaccgc
    21901 agacttcgcg gaaaaggccg cccgggcggc cctggcggac gccggggtgg atcccaaaga
    21961 tgtcggatta ttcgtcgtgg gcacggacac cccggagtac gtgagccccg ccaccgccat
    22021 cctggtccag ggacgtctgc agtgcggtga gacggacgcg ggggcctacg acgtgggagc
    22081 ctcctgcgcc tccttcacca tagccctgga ccaggccgcc cgggtcctgg ctacggagcc
    22141 cggcctccgc tacgccgtgg tcgcaggggt ctacgacatg cccgcccaca tccggcccgg
    22201 agacgccttc gggtggtcca tcttcgcgga cggggcgggg gccgcggtcc tggagcgggt
    22261 ttccgacgac gatctttccg gctacgtcac gggccgccaa aggaccgacg gcacccagtg
    22321 gaacttcatc ggggtctacg ccgggggaac ccggaagccc gtgacccggg agctcctgga
    22381 ggccgggcag tacgg
//
LOCUS       DAOTEI010000038        21910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1574_length_21910_cov_4.866712, whole genome shotgun sequence.
ACCESSION   DAOTEI010000038 DAOTEI010000000
VERSION     DAOTEI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 21910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21910
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1574_length_21910_cov_4.866712"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="P5313_05590"
     CDS             <1..800
                     /locus_tag="P5313_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HRY79871.1"
                     /translation="MLNDSMYGILFRDINMYRTFVDQKFSRMINAYAGIIINTGEDNY
                     LTTSDAYEKAYTVLASQFLNERFAYAAGLPARLMGLGHAFEMDPTIKDGFLYELAQAQ
                     MAREIFPEHPLKYMPPTKFMSGDIFKGLTMDMMFNFVSKATDQGIHLLGMLTEAIHTP
                     FMMDRYLAVDNARYVMNNMASFYEEVEFREGGIVRTRAAEVLDKTLEFLRQTEAAGLF
                     DAIAQGHFADVKRPKDGGKGLEGLVKKGPRYWNPFEEHLTRTLGLGK"
     gene            811..1542
                     /locus_tag="P5313_05595"
     CDS             811..1542
                     /locus_tag="P5313_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HRY79872.1"
                     /translation="MNIVRPYGDTLDDGKVQLSFTLPVPDGAKGREAARQLVLKWGFK
                     DCDVVHSAPLSAGFSFYVAYGKTESSVDYDSCVADEHSAEASLSMDEVNEFIRREIRR
                     KVVVVGACTGFDAHTVGIDAIMNMKGYNHHYGLERYSELEAYNLGAQVPNEKLIEFAG
                     KVKADAILVSQVVTQKDVHIHNLTQLIELLEARGERGRYICVAGGPRIGNKLAVELGY
                     DVGFGRGAYANDVATFIVKKLAERN"
     gene            1650..3287
                     /locus_tag="P5313_05600"
     CDS             1650..3287
                     /locus_tag="P5313_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRY79873.1"
                     /translation="MELTHYGLLSILPPLLTIVLAVVTKDVIVSLFLGILSGTLIAAG
                     GNPYTALINLSDLIADTLADGWNIRIMLFTILLGLLVGLLAKSGAAYALGDWAGKKIK
                     SRTGALIFTWFFGILIFFDDYFNSLTIGTCMRPLFDAKKISRAKLAYILDSTAAPVCI
                     IAPISGWVAFVIGTYKAMPEWQGLGVGELTFFLRTIPFNLYAIFAVFMVLFISITHKD
                     FGPMARSEARAEKGLGLYDEEKYGVVVSQVESKAHTSNAKPIDFVIPILSVIVIALFF
                     FPMVTWMGAVDGEAIKSVGQAMAEIPLGQAFNDTDSSKALMYALIFSTCFSYVYMIAR
                     KLLNITAAGEALLDGFRAMVPAAMILTLAWSIGTIIKTPRADGGVGLGLYVADVVVNG
                     NFPMFLFPAVVFVLACIISFAAGTSWGTMGIMVPVALPIIVQLSKMAGLSPAATVNAT
                     ALTIGVVLGGAVFGDHCSPISDTTILSSTGAASPHLEHVATQLPYALFVAACALLGTV
                     AGGLTWNPFVALIVTAIPFVAGTLLLPNWFGPKKYGL"
     gene            3494..4573
                     /gene="buk"
                     /locus_tag="P5313_05605"
     CDS             3494..4573
                     /gene="buk"
                     /locus_tag="P5313_05605"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRY79874.1"
                     /translation="MAEKMFRILAINPGSTSTKIAIYENEKETRRESIEHPRSEIEKY
                     KAIADQYPMRKDAVLAFLRSAGLAEKDFDAIVARGGTLPPVRSGAYRINRLMVETLRD
                     RPQAEHVSNLAAMIAFEIAQGAGIPSFIYDSVAVDELDDAARLTGLAEIKRRSLSHAL
                     NMRAAAIRTAARLGRPYGELNLVVAHLGGGITISVHRKGRMADIVSDDEGPFSPERAG
                     RLPALALVDLCYSGKYEKSALKKLCRGAGGLVSLLGTADAQEVERRIAAGDRDAELAY
                     RALAYQVAKGIGELATVVDGEVDRVVLTGGIAHSKLLTGWIEKSVKFIAPVELMPGEN
                     ELESLALGALRVLRGQEAALDFDVR"
     gene            4606..5901
                     /locus_tag="P5313_05610"
     CDS             4606..5901
                     /locus_tag="P5313_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY79875.1"
                     /translation="MATRPGSRAAGAPGVPILIPAVILQLCVGILYMWGVFQPHVVRY
                     FGWDFQVVARTSSLMLFAFVAGTLTGGFLLDRLGTRPVVTLGSAVFSAGILASSFLGG
                     GDPRLIHLTYGLVAGTGTGLVYGGVVSCVQRWYPRRRGFAAGIAAGAFGFSVVLLSPI
                     AEALISRVGLPGTFRILGAVFLAATLSASRFVSDPPETSAPAGIASSSPGGPSGPSAP
                     GGPVGTAGAAGGHAPEPRQYRPREVLASPIFWCAFLCLFLEVAAWVVILPVIKTLALA
                     RGMSRELALASVMVTGIANASGRIVSAWISDRIGRTRTIVALCLLTCGASLWLVGAVG
                     IPYMVAVFLVAFAYGGPSGVFPPLVREVFGQRYAGTNFGMVLMALGFSSLVFSRVSGI
                     LSAGGAASGDYSAIFYLTAALGLAAAGFMLAAERMIGRR"
     gene            6077..6745
                     /locus_tag="P5313_05615"
     CDS             6077..6745
                     /locus_tag="P5313_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRY79876.1"
                     /translation="MEKPVINAAEAAKMVNEGDVVHVGGFLACGSPLGILAALAARGT
                     KNLTLVCNDTAVLDEKNGRVTGVAPLVKNRQFRKMIISHIGTNPESQRQMNAGETEVV
                     LVPQGTLAERVRAAGCGLGGILTATGLGTEVQEGKQVVEVQGRTFLVEEALPGDVALI
                     KARKADKAGNLVYSKTARNFNPLMAMACKTVIVEAEEIVNIGEIDPDQVHTPSILVDY
                     LVKA"
     gene            6758..7420
                     /locus_tag="P5313_05620"
     CDS             6758..7420
                     /locus_tag="P5313_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRY79877.1"
                     /translation="MEYVESKENIARRIARIFKTGDVVNLGIGLPTLVANYVPPEVTV
                     ILQSENGLIGLGPAPEKGREDKDLTNAGAMPVTLLTGGAYFDSAMSFGIIRGGHVDCT
                     VLGVLEVDQEGNLANYKIPGKMVPGMGGAMDLVAGSRVVVAATTHLEKTGGSKLKRRC
                     SLPLTAEHGVDIVVTDVGYFEVKGSHFVLREYFEPYTPEWIVEHTDADVQVSPDCKPW
                     RA"
     gene            7452..8633
                     /locus_tag="P5313_05625"
     CDS             7452..8633
                     /locus_tag="P5313_05625"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRY79878.1"
                     /translation="MKDVFIVEAVRTAIGNFGGAFTATGAVDLGAAVVKALLERTRVG
                     AAEVDEIVLGCVLQAGLGQNPARQVLVKAGLPVERTAMTVNMVCGSGLRAVAAAAQAV
                     KAEDAALVIAGGTENMTQAPYLLKQARYGYRMGHGELVDSMIFEGLTDIFNGYHMGIT
                     AENLAAKYGLSREAQDAFAAASQNKTEKAIAAGRFKDEIVPVYLPQKKGDPIVVDKDE
                     FPRPGVTAEALGKLRPAFKPDGTVTAGNASGINDGAAAVMVADQEEMERHGLEPLARI
                     ASYGWHGCDPSIMGIGPVEAVRLALSRAGWTLADVDLIEANEAFAAQSLSVAKELGFD
                     LEKVNVNGGAISLGHPIGASGARILVTLLHEMRRRKAKKGLATLCIGGGMGIAMCVER
                     P"
     gene            complement(8751..10088)
                     /locus_tag="P5313_05630"
     CDS             complement(8751..10088)
                     /locus_tag="P5313_05630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRY79879.1"
                     /translation="MAIPVLMPQLGLTMTEGLLTEWSKKEGDEVRKGDLLFSVENDKA
                     VLPVESQADGILANVSARAGDVVPVGTPVALILAAGETAPTSAQADSGARPAAAPAVP
                     AAWSTAAPAPAEAPSREAAPAAPIPTTAAVPKTAESPTQKATDGFVLASPRARHLASA
                     AGLDLGSIPGTGPGGAVVERDLPVSARAADPGAGAVSAASRPSASPSPGTFAAAPGLP
                     GGSELVTLPRVGQVGAERMAKSWAEIPQFTLYAEACASELLRIHARGKEAGNPASVTV
                     LLAGLLARALRDFPRLNASWEGNGRVRTYRAVNVGVAVDGPDGLVVPVLRDCGIKGFS
                     ELARELAGLAERAKTRTLYSDDLSGGTVTLSNLGMFGIKRFRAIVNPPQTAILAAGAV
                     ERKPVETEDGDIRFLPYLEISVTADHRAVDGAYAARFLARYKELIEDPQFALF"
     gene            complement(10090..11070)
                     /locus_tag="P5313_05635"
     CDS             complement(10090..11070)
                     /locus_tag="P5313_05635"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012859516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRY79880.1"
                     /translation="MRTITFSDATLEAMKEEMRRDPTVFVYGEDIVRQGGIFGQFKGL
                     PEEFGDRIRDVPISETAIIGAALGAALTGTRPVADMHFADFISVAYDELVNQIAKNRY
                     MFGGQANIPLVVRAPDGIINQAAAQHSQSVEGWLMNVPGLILVAPSSPATAKGLLKSS
                     IRQNDPVIFFEHKVLYPQKGPVPEGEYLTPLGKAEIRRPGTDVTVVTYSLMVQKCEEA
                     AKALEAKGISVEIVDLLTLKPMDTATILDSVRKTHRCVVVHEGVVNGGLGAEIAARVQ
                     AEAFYHLDAPVLRVGAKDVPIPFSPPLEKYVAANLSDIERAVLRVLHREA"
     gene            complement(11067..12098)
                     /locus_tag="P5313_05640"
     CDS             complement(11067..12098)
                     /locus_tag="P5313_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRY79881.1"
                     /translation="MPSRIKEFPNRFAELPASDKVGLLRTMVRIRAFETRAEMLFMNK
                     KIPGFVHLYIGEEAIATGVMANLRKDDWITSTHRGHGHVLAKGADTKRMMAELFGKKT
                     GFCKGKGGSMHIADFSVGMLGANGVVGGGYNIAVGAGMSAQIKKTDQVAVCFFGDGAS
                     NRGTFHEALNLASIWKLPVLFVNENNRYASTTPTGYHLSSARVADRAKGYGIEGYTVD
                     GNNVVEVYDAATTLIENARKGKGPAILECLTYRIKGHYVGDPEKYRTREEVQAQQREN
                     DPIERYFAFLAGKKAADEATLSALRAEAEREIDEAVAFAESSPLPDPEDALDDLFSFS
                     PSEFPEAVR"
     gene            12274..13677
                     /locus_tag="P5313_05645"
     CDS             12274..13677
                     /locus_tag="P5313_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY79882.1"
                     /translation="MIQWTHTSRVVDVDFEVDGADLFKSSPATIMKTIIDSISDGIII
                     LDKSGTVLYMNQEGARILGLNQEGVIGKNVSDIVDFRPVILEVLESGKGYIEKEFIIH
                     SPSRGQLHFIKSAVILHDKAGNMIGVIDTFRQVEKVHTMIARMTGAKAKFTFQDIVGE
                     DPSFREAVNLSRMAAQSRANVLIEGESGTGKEMFAHAIHHASDRSAGPLIIVNCAALP
                     RSLVESELFGYESGSFTGARKEGFAGKFEQANMGTLFLDEIGELPLDMQAKLLRVLQD
                     RTFSRIGGNKSITVDTRVIAATNKNLLSEIRNNAFREDLYYRLNVVNIHFPSLRERPG
                     DIALLAAHFAQKSAAYYGKPVYEIDSEVLRLFRGYPWPGNVRELENVIERAMIVAKGH
                     RITPADLPPHVAAMECAPGTKPSACRDQEVLNLDRVERDEIQRALRVCEGNISSCAKL
                     LGISRNTLYRKMKKYEI"
     gene            complement(13930..15222)
                     /locus_tag="P5313_05650"
     CDS             complement(13930..15222)
                     /locus_tag="P5313_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY79883.1"
                     /translation="MAALIVFGLFVLLIAFGAPIGIALGAAGIGAILNFDLGVAMVSR
                     NIEANIAKFPLIAIPFFILAGNLMEKSRIVDGISKFVILLTGKRTGGLAIAGVITCIF
                     WGAVSGSGPATAAALGMVFIAPMVKQGYDKYYAASTIAAGAGLSIIIPPSIAFIVYGN
                     LTNVSVGALFLGGILPGIVVGIFLIVAVYLGARKHGFRGSVASGTPKEILAAFLESFW
                     ALLAPVIILGSIYAGIATPTESAIMGVFYALFVGLFIYKTINLDVLMKALIDTVQGSA
                     VVMFVVSMAGIFTWAASTLGVIDAAANLVSSLTKNPLVFVMLCNVVILIAGMFLDAIS
                     ITYVFMPIILPALLALGIDPLYFGIVFVVALAIGQITPPVAVNLYVTANLIGSNINDR
                     MVKFVIPMVVSAVLALIFLTLIPQISLWLPVASGLYTP"
     gene            complement(15222..15704)
                     /locus_tag="P5313_05655"
     CDS             complement(15222..15704)
                     /locus_tag="P5313_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY79884.1"
                     /translation="MEKKKYHAEEVIGAVLLAGMSVLTFLNVINRYVFKRSIAFTEEI
                     TVNLFVWITLIGIAMAFRKGANLRMTNLFDRFGPKLQKAATVGAGVIGILIFGFVIVN
                     SCQEIWKNMTFYRTTSEALGIPTWIYSLGTPIFSGMVIWRILRATFPRNKADKAEGGA
                     "
     gene            complement(15899..16972)
                     /locus_tag="P5313_05660"
     CDS             complement(15899..16972)
                     /locus_tag="P5313_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY79885.1"
                     /translation="MKRTRFLTVLAALALFSLAAGMVAAQGSFKPKAEYTMQVNVGPA
                     FDWGIGAQRWADLVKEKTGGRVNVKPYFGSSLLAGKQTNWYQAVADGSIDFVVESTIN
                     ASTVIRAFNLFNLPFFLGTYENVDKIQAGKAGAAIVKEFERGGIKFLAWGENGFRQVT
                     NSKREIKTPQDLAGLKLRVVGSPIFVDIFKQFGADPVNMNWADAVTGFQQKVVDGQEN
                     PKSVLTVVKIWEYHKYMTNWNYVLDPLVFGVNMKVWNSFPKDIQKAIEEAAVEAAGYQ
                     KALARAHLDGTKSLDILKTKYKVTEMPIEPFKLAVDNGMVITNLTPAQMQAFKDATKP
                     IYDKWIPQIGKNIYDLAVQDMNK"
     gene            17313..18704
                     /gene="lpdA"
                     /locus_tag="P5313_05665"
     CDS             17313..18704
                     /gene="lpdA"
                     /locus_tag="P5313_05665"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRY79886.1"
                     /translation="MEDKTYDVAILGAGPGGYVAAIRARQLGLSAVVIEKDKPGGVCL
                     NIGCIPSKALIHQAQVFSSAAGLRELGATVDLSGFDYGKVFRKSRMAADRLSKGVEFL
                     LRKNKVDFVAGTGAFKSADRLIVDGKTEIAFKNAIVATGSRPREIPGFAFDGKVVLSS
                     TDALMLEKLPRRLVILGAGAIGMEFAYVMNSFGVEVTLVEMLDTILPLEDSETVEVVR
                     RDFVKKGVKILTGTRAEGLERKPEGAVVRLSGKDGAKTEVEADQVLVAVGRAPNTENL
                     GLEALGIRTEKGFIPVGDYGQTAVPSIYAIGDVVATPLLAHVASKEGEIAVERMAGKD
                     PERRVALDEIPSAVYCEPQVGSFGLTEREAVERGVRFQKAVFPYRGIGKAVAVEALDG
                     IVKILHDPQTGEILGAHVAGAEATELVHELLLAKKSELTVQDIAGMIHAHPTLSEGVM
                     EAARLAEGWAIHI"
     gene            complement(18769..19467)
                     /locus_tag="P5313_05670"
     CDS             complement(18769..19467)
                     /locus_tag="P5313_05670"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRY79887.1"
                     /translation="MEKKRAELVRTLTNLIREPGRFPEGRLPPERELAESLGVSRNLL
                     REAIVTLEALGFLEIRERQGAFITPPGTEDFAASLKFLSIWPEDLLSYLMEMRLVIEV
                     PAAGLAAERRTDAELARMRDCVAHLEAEQDNPDGGLSSGAVWDTLLHTLVLDAAHNPV
                     LSRMYEGLSSTMEKYIVLSRSKLLALGGWSGKILDEHRRLVEAIAARDPAAAQDALRR
                     HLDGALRKLNELNR"
     gene            19548..20504
                     /locus_tag="P5313_05675"
     CDS             19548..20504
                     /locus_tag="P5313_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY79888.1"
                     /translation="MRKVAAIISLACLAVSAFALDIKLAHVVNEQDSFHLAAVKFKEL
                     TEKYTNGAVKVTIFPNATLGDERTLLERMKMGIVDAGIITNGPIINFVPRFGVIDLPF
                     LFRSPEHAYKVLDGSIGQSLFADLEAQGWKGLAWAERGFRNLTNNRKPVQSPKDMEGL
                     KIRVMQNPVYVDSFKALGANAVPMAWTEALTALQQKTIDGQENPLNVIVAFKLDESQK
                     YMSITRHAYAPAPILMSMMTWKKLSAPQQEAVLKAAREAAQFERDFNNSSEAQWMKDL
                     AAKGMVISTPDLKPFLDSVKPVYDSYADKFGKDLINSILAVK"
     gene            20514..21023
                     /locus_tag="P5313_05680"
     CDS             20514..21023
                     /locus_tag="P5313_05680"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY79889.1"
                     /translation="MSSATERRGAQAPFLHAASDAVNLWSERFLFVLMLAMVGVTTAQ
                     VVFRFFFQALTWSEELSCFLLVLASLVGSAVAFKKGSHIAVTFIADRLPETGRRLLAT
                     LVHLLGLVFFLIVAYYGAVLMKSEAGQTTPALQISMKWVYLMYPVAGAATALHLLDGM
                     FRVWTGGES"
     gene            21020..>21910
                     /locus_tag="P5313_05685"
     CDS             21020..>21910
                     /locus_tag="P5313_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY79890.1"
                     /translation="MMGWIIFGSFFLLMLIGVPIGLAIGLASLIVFLASGFPLQMVPQ
                     TLLEGNNSFALVAVPFFILAGDLLAKGGISERIVSFAEASLGKRRGGLSIVSVLASMF
                     IAAISGSGAATTAAVGSALLPDLKEKKYDVDFSAALIASAGTIGVVIPPSVPMVLYAV
                     IAGESVAKLFMGGFIPGTLMGAGLIAYAVWYSNRRGYPASEKLPPRAVARRFFDSLWG
                     LATPVIILGGIFTGYFTPSEAAAMAVDYTLLIAWLVYRTMTFRRFYELVVGAGVTSAL
                     IMFIIGTAKIFGWGLAFYEIP"
BASE COUNT         3666 a         7211 c         7270 g         3763 t
ORIGIN      
        1 tgatgctgaa cgactcgatg tacgggatcc tcttccgcga catcaacatg taccgcacct
       61 tcgtggacca gaagttcagc cgcatgatca acgcctacgc ggggatcatc atcaacaccg
      121 gcgaggacaa ctacctgacc accagcgacg cctacgagaa ggcctacacg gtcctggcca
      181 gccagttcct gaacgagcgc ttcgcctacg ccgcgggcct gcccgcccgg ctcatgggcc
      241 tggggcacgc attcgagatg gacccgacca tcaaggacgg cttcctgtac gagctggccc
      301 aggcccagat ggcccgggag atcttcccgg agcatcccct gaagtacatg ccccccacca
      361 agttcatgtc cggggacatc ttcaagggac tgacgatgga catgatgttc aacttcgtgt
      421 ccaaggccac ggaccagggc atccaccttt tgggcatgct gaccgaggcc atccacaccc
      481 ccttcatgat ggaccggtac ctggcggtgg acaacgcccg gtacgtgatg aacaacatgg
      541 cctccttcta cgaggaagtt gagttccggg agggcgggat cgtccgcacc cgggcggcgg
      601 aggtcctgga caagaccctg gagttcctgc ggcagaccga ggcggcgggc ctcttcgacg
      661 ccatcgccca ggggcacttc gcggacgtca agcggcccaa ggacgggggc aagggcctgg
      721 agggcctggt caagaagggg ccgaggtact ggaatccctt cgaggaacac ctgacccgga
      781 ccctggggct gggaaagtga ggaggctgac atgaatatcg tccgaccgta cggagacacc
      841 ctggacgacg ggaaggtcca gctctccttc accctgccgg tccccgacgg ggccaagggc
      901 cgcgaggccg cccggcagct ggtcctcaag tggggcttca aggactgcga cgtggtccac
      961 tcggcccccc tgtccgcggg tttctcgttc tacgtggcct acggcaagac cgagtcctcg
     1021 gtggactacg actcctgcgt ggccgacgag cactccgccg aggcctccct cagcatggac
     1081 gaggtgaacg agttcatccg ccgggagatc cgccgcaagg tcgtggtggt gggggcctgc
     1141 acgggtttcg acgcccacac ggtggggatc gacgccatca tgaacatgaa gggctacaac
     1201 caccactacg ggctggagcg atactccgag ttggaggcct acaacctggg cgcccaggtc
     1261 cccaacgaga agctcatcga gttcgcgggc aaggtgaagg cggacgccat cctggtttcc
     1321 caggtggtca cccagaagga cgttcacatc cacaacctca cccaattgat cgagctgctg
     1381 gaggcccggg gcgaacgggg ccgctacatc tgcgtggccg ggggcccccg gatcggcaac
     1441 aagctggcgg tggagttggg ctacgacgtg ggattcggcc ggggcgccta cgcgaacgac
     1501 gtggcgacct tcatcgtgaa aaagctggcg gaacggaact gatgcccgcg tccgtgccca
     1561 aaacaagaaa ttaccgttgc gttttcggtt tttcgggtgc atcatggggc ggtatgtccg
     1621 ttccattcca tcaaaggagg cactagacca tggaattgac gcattatggg ctactctcca
     1681 tcctgccgcc cctcctcacc atcgtcctcg cggtggtgac gaaggacgtc atcgtctccc
     1741 tcttcctggg catcctgtcc gggacgctga tcgcggcggg agggaacccc tacaccgccc
     1801 tgatcaacct gtcggacctg atcgccgaca ccttggcgga cgggtggaac atccgcatca
     1861 tgctcttcac gatcctgctg ggcctcctgg tcggcctgct cgccaagtcc ggcgcggcgt
     1921 acgccctcgg ggactgggcc ggcaagaaga tcaagtcccg gaccggggcc ctgatcttca
     1981 cctggttctt cggcatcctc atcttcttcg acgactactt caattccctg accatcggca
     2041 cctgcatgcg gcccctcttc gacgccaaga agatctcccg ggccaagctg gcctacatcc
     2101 tggactccac ggcggccccg gtctgcatca tcgcccccat ctccggatgg gtggccttcg
     2161 tgatcgggac ctacaaggcc atgcccgagt ggcagggcct gggcgtgggc gagctgacct
     2221 tcttcctccg gaccatcccc ttcaatctct acgcgatctt cgcggtcttc atggtcctgt
     2281 tcatcagcat cacccacaag gacttcggtc ccatggcccg gtccgaggcc cgggcggaga
     2341 aggggctggg gctctacgac gaggagaagt acggcgtcgt ggtcagccag gtcgagtcca
     2401 aggcccacac ctccaacgcc aagccgatcg atttcgtgat tcccatcctc tcggtcatcg
     2461 tgatcgccct cttcttcttc ccgatggtca cctggatggg cgccgtggac ggcgaggcca
     2521 tcaagagcgt cggacaggcg atggcggaga tccccctggg acaggccttc aacgacacgg
     2581 attcctccaa ggccctcatg tacgccctga tcttctccac ctgcttcagc tacgtctaca
     2641 tgatcgcccg gaagctcctg aacatcaccg cggccggaga ggccctgctg gacgggttcc
     2701 gcgccatggt acccgcggcc atgatcctga ccctggcctg gtccatcggc acgatcatca
     2761 agaccccccg ggccgacggc ggcgtgggtc tgggcctgta tgtcgcggac gtggtcgtga
     2821 acggcaactt cccgatgttc ctcttccccg cggtggtctt cgtgctggcg tgcatcatct
     2881 ccttcgccgc gggtaccagc tggggcacca tgggcatcat ggtccccgtg gccctgccca
     2941 tcatcgtcca gctgtccaag atggccggcc tctccccggc ggccacggtc aacgcaacgg
     3001 ccttgaccat cggcgtggtc ctgggcggtg cggtgttcgg ggaccactgc tccccgatct
     3061 cggacaccac gatcctgtcc tccacgggcg cggcttcccc gcacctggag cacgtggcca
     3121 cccagctgcc ctacgccctc ttcgtggcgg cctgcgccct cctgggcacc gtcgcgggcg
     3181 ggctcacctg gaatcccttc gtggccctga tcgtgacggc cattcccttc gtggcgggca
     3241 ccctcctgct gcccaactgg ttcggcccca agaagtacgg gctgtaagcc tttccgacct
     3301 ttccgcccgc cccgcgatcg cggggcgggc gcgcgggccg ttccggacac cggcaagggg
     3361 ttcggggcgg cccgcgtttc atttcccgaa gcccgggccg cggtccttga cgccccggcg
     3421 gcgatccatg gaataatgaa gcccagcgac gccggccgcc cccccggcgg cacgaagcga
     3481 ccggaggttc ccgatggcgg aaaagatgtt ccggatcctg gcgatcaatc ccggatccac
     3541 gtccacgaag atcgcgatct acgagaacga gaaggaaacc cgccgggaaa gcatcgagca
     3601 tccccggtcg gagatcgaga agtacaaggc catcgcggac cagtacccga tgcgcaagga
     3661 cgcggtcctg gccttcctgc gttccgcggg ccttgccgag aaggatttcg acgccatcgt
     3721 tgcccggggc gggaccctgc cgcccgtgcg ctccggagcc taccggatca accggctcat
     3781 ggtggagacc ctgcgggacc ggccccaggc ggagcacgtc tccaacctgg cggccatgat
     3841 cgccttcgag atcgcccagg gcgcgggcat cccctccttc atctacgact ccgtggcggt
     3901 ggacgagctg gacgacgcgg cccggctcac ggggcttgcg gagatcaagc gccggagcct
     3961 ctcccacgcc ctgaacatgc gggccgccgc catccggacc gccgccaggc tcgggcggcc
     4021 ctacggggag ctcaacctcg tcgtggccca cctgggcggg ggcatcacga tctccgtcca
     4081 ccggaaaggc cggatggcgg acatcgtctc cgacgacgag gggcccttct ccccggagcg
     4141 ggcgggccgc ctgcccgccc tggccctggt cgacctgtgc tattcgggga agtacgagaa
     4201 gtccgccctc aagaagctgt gccggggcgc agggggcctg gtatccctcc tgggcaccgc
     4261 ggacgcccag gaggttgagc gccgcatagc cgcgggggac cgggacgccg agctggcgta
     4321 ccgcgccctg gcctaccagg tggccaaggg catcggcgaa ctggccaccg tggtggatgg
     4381 cgaggtggac cgggtcgtgc tgaccggagg catcgcccac tcgaagctcc tgaccggctg
     4441 gatcgagaag agcgtgaagt tcatcgcccc ggtggagctg atgcccgggg agaacgagct
     4501 ggagtccctg gccctgggcg ccctccgggt cctccggggc caggaggccg ccctggactt
     4561 cgacgtccgg tgagggcgga gacgccggaa ggtaccggaa tcgacatggc gacacgaccc
     4621 ggatcccggg cggccggagc gccgggagtg cccatcctga ttcccgcggt catcctccag
     4681 ctctgcgtgg ggattctcta catgtggggg gtcttccagc cccacgtggt ccggtacttc
     4741 ggatgggatt tccaggtggt cgcccggacc tcctccctca tgctcttcgc cttcgtggcg
     4801 ggcaccctga ccggcggatt cctcctggac cgcctgggca cccgtcccgt ggtcaccctg
     4861 ggatccgccg tcttctcggc cggcatcctt gcctcctcct tcctgggggg cggcgacccc
     4921 cggctcatcc acctgaccta cggcctcgtg gcggggaccg ggacgggact ggtctacggg
     4981 ggcgtggtgt cctgcgtcca gagatggtat ccccggcgga gaggttttgc cgcgggcatc
     5041 gccgcagggg ccttcgggtt ttcggtggtc cttctcagcc cgatcgcgga ggccctgatc
     5101 tcccgggtcg gcctgcccgg gaccttccgg atcctgggcg cggtcttcct ggccgctacc
     5161 ctgagcgcgt cccgtttcgt ctcggatccc ccggagacct ccgcgcctgc gggcatcgcc
     5221 tcctcctccc cgggcggccc ttccgggccc tcggcccccg gcggcccggt cggaacggcg
     5281 ggagcggccg gagggcacgc tcccgagccg cggcaatacc ggccccggga agtcctggcc
     5341 agcccgatct tctggtgcgc ctttctttgc ctcttcctgg aggtggccgc ctgggtggtg
     5401 atcctcccgg tcatcaagac cctggccttg gcccggggga tgtcccggga gctggccctg
     5461 gcctcggtga tggtgaccgg aatagccaac gcctcgggac ggatcgtctc ggcctggatc
     5521 tcggaccgga tcggccggac ccggaccatc gtcgccctgt gccttctgac ctgcggggcc
     5581 tccctttggc tggtcggcgc ggtcgggatc ccctacatgg tcgccgtctt cctcgtcgcc
     5641 ttcgcctacg ggggcccctc cggagtcttc ccgcccctgg tccgggaggt cttcggccag
     5701 cgctacgcgg gcaccaactt cgggatggtc ctgatggccc tgggattctc ctccctggtg
     5761 ttctcccggg tctccggaat cctttccgcg ggtggagccg cctcggggga ttactccgcc
     5821 atcttctacc tgaccgcggc cctggggctg gcggccgcgg gattcatgtt ggcggcggag
     5881 aggatgatcg ggcggaggtg aatgggtgaa tgggtgaatg ggtgaatggg tgaatgggtc
     5941 ctgcctctat tcacctattc actaattcac ttattcacca atgcttcccc gaatttgctt
     6001 caccgcggat tccatggtac cctatcttcc ccgggatctc ccgggcactc gatagaatcc
     6061 tggaggtctc tccgtgatcg agaaacccgt gatcaacgcg gccgaggccg cgaagatggt
     6121 gaacgagggc gacgtggtcc acgtcggggg attcctggcc tgcgggtccc ccctgggaat
     6181 cctggcggcc ctggcggctc ggggcacgaa gaacctgacc ctggtctgca acgacaccgc
     6241 cgtgctggac gagaagaacg gccgcgtcac cggggtggcc cccctggtga agaaccggca
     6301 gtttcggaag atgatcatat cccacatcgg caccaacccc gagtcccagc ggcagatgaa
     6361 cgccggggag accgaggtgg tcctggtccc ccagggcacc ctggccgagc gggtgcgggc
     6421 cgcgggctgc ggcctgggcg gaatcctgac cgccaccgga ctgggcacgg aggtccagga
     6481 gggaaagcag gtggtcgagg tccagggccg gacgttcctg gtcgaggaag ccctcccggg
     6541 ggatgtggcc ctgatcaagg cgaggaaggc cgacaaggcg gggaacctcg tatattccaa
     6601 gaccgcccgg aacttcaacc ccctcatggc catggcctgc aagaccgtca tcgtcgaggc
     6661 ggaggagatt gtgaacatcg gagagatcga tccggaccag gtccacaccc ccagcatcct
     6721 cgtggactac ctggtgaagg cctaggaggg cgccatgatc gaatatgtcg agagcaagga
     6781 gaacatagcc cgccggatcg cccggatatt caagaccggg gacgtagtca acctcgggat
     6841 cggcctgccc accctggtgg ccaactacgt cccgccggag gtcacggtga tcctccagag
     6901 cgagaacggc ctcatcggcc tcggacccgc gccggagaag ggtcgggagg acaaggactt
     6961 gaccaacgcc ggggccatgc ccgtgaccct gctgaccggg ggggcctact tcgactcggc
     7021 catgagcttc ggtatcatcc ggggaggaca cgtggactgc acggtcctgg gggtcctgga
     7081 ggtcgatcag gaaggcaacc tggccaacta caaaataccg ggcaagatgg tgcccggcat
     7141 gggaggggcc atggatctgg tggcgggctc ccgggtcgtg gtggccgcca caacccacct
     7201 ggagaagacc gggggatcca agctcaagcg ccgctgttcc cttcccctta ccgccgagca
     7261 cggggtggac atcgtcgtga ccgacgtggg gtatttcgag gtcaagggca gccacttcgt
     7321 gttaagggag tacttcgagc cctacacccc cgaatggatc gtggaacata cggacgcgga
     7381 cgtacaggtt tcgccggact gcaagccctg gcgagcctag gccgacggtc ccgcaaagca
     7441 aggaggaaat tatgaaggac gtattcatcg tcgaggccgt tcgcacggcc atcgggaact
     7501 tcgggggagc gttcacggcc acgggcgccg tggacctggg tgcggcggtg gtgaaggccc
     7561 tgctggagcg caccagggtc ggggcggcgg aggtggacga gatcgtcctg ggctgtgtcc
     7621 tccaggcggg gctggggcag aacccggccc gccaggttct ggtcaaggcg gggctcccgg
     7681 tcgagcggac cgcgatgacg gtcaacatgg tctgcggctc cggtctgcgg gccgtggccg
     7741 cggcggccca ggcggtcaag gcggaggacg ccgccctggt catcgccggt ggcaccgaga
     7801 acatgaccca ggctccctac ctcctcaagc aggcccggta cggctaccgc atgggccacg
     7861 gggagctcgt ggactccatg atcttcgagg ggctcacgga catcttcaac ggttaccaca
     7921 tgggcatcac cgcggagaac ctggccgcca agtacggcct gtcccgggaa gcccaggacg
     7981 ccttcgccgc ggccagccag aacaagaccg agaaggccat cgcggccggc cgcttcaagg
     8041 acgagatcgt ccccgtgtac ctgccccaga agaaggggga tccgatcgtc gtcgacaagg
     8101 acgagttccc ccggcccggg gtgaccgcgg aggccctggg caagctccgg cccgccttca
     8161 agcccgacgg caccgtgacc gccggcaacg cctcggggat caacgacggg gccgcggcgg
     8221 tgatggtggc ggaccaggag gagatggagc gccacggcct tgaacccctg gcccggatcg
     8281 cttcctacgg ctggcacggc tgcgacccct cgatcatggg gatcggcccc gtggaggccg
     8341 tgcgcctggc cctctcccgg gcgggctgga ccctggcgga cgtggacctg atcgaggcca
     8401 acgaggcctt cgcggcccag tccctctcgg tggccaagga gctcggcttc gacctggaga
     8461 aggtcaacgt gaacggcggg gccatctccc tgggccaccc catcggagcc tcgggagcgc
     8521 ggatcctggt gaccctcctc cacgaaatgc gccgccggaa ggccaagaag ggcctggcga
     8581 ccctctgcat cgggggagga atgggcatag cgatgtgcgt cgagcggccg tagtcgaaaa
     8641 gacagtcgaa tcgccgaagc aagcttcggc gccgccgggc cggcaacgcc ggcccggtgt
     8701 aaacgaggcc ccgggttcga cccggggcct cgctacttca gctagagcgt tcagaacagg
     8761 gcgaactggg gatcctcgat cagctccttg taccgggcca ggaagcgggc cgcgtaggcc
     8821 ccgtccacgg cgcggtggtc ggcggtgacc gatatctcca ggtagggcag gaagcggatg
     8881 tccccgtcct cggtctccac gggctttcgc tccacggccc ccgcggccag gattgccgtc
     8941 tggggcgggt tcacgatggc ccggaagcgc ttgatcccga acattccgag gttggagagg
     9001 gtcaccgtcc cgcccgacag gtcgtccgag tagagggtcc gggtcttggc ccgctccgcg
     9061 agcccggcca gttcccgggc cagttcggaa aagcccttga tcccgcagtc ccggaggacc
     9121 ggaaccacca ggccgtccgg cccgtccacc gctaccccca cgttcacggc ccggtaggtc
     9181 cggaccctgc cgttcccctc ccaggaggcg ttgagccggg ggaagtcccg gagggcccgg
     9241 gctaggagcc ccgccaacag caccgtgacc gaggccggat tgccggcctc cttgccccgg
     9301 gcatggatgc gcaggagttc ggatgcgcag gcctccgcgt acagggtgaa ctgggggatc
     9361 tccgcccagc tcttggccat gcgttcggcg ccgacctggc cgacccgggg gagggtgacc
     9421 agctcggagc cgccgggaag cccgggcgcg gccgcgaagg tccccgggct cggcgaggcg
     9481 gacggccggg atgcggcgga aacggcgccg gcccccgggt ccgctgcccg ggcggaaacc
     9541 ggcagatccc gctccaccac ggcccctccg ggacccgtgc cggggatcga gcccaggtcc
     9601 agcccggccg ccgaggccag gtgccgggcc cggggagacg cgaggacgaa accgtccgtc
     9661 gccttctgag tcggcgactc ggcggttttc gggacggccg ccgtggtcgg aatcggggcc
     9721 gccggggccg cctcccggga gggggcttcg gccggggccg gagcggccgt cgaccaggcg
     9781 gccggaaccg ccggggccgc ggccggacgg gcgccgctgt ctgcctgggc cgacgtcggg
     9841 gccgtttcgc ccgcggccag gatcagggcc accggggtcc ccacggggac cacgtccccg
     9901 gcccgggcgc tcacgttcgc gaggatcccg tccgcctggg actccacagg gaggacggcc
     9961 ttgtcattct ccacgctgaa taggaggtcg ccctttcgga cctcgtcccc ttccttcttc
    10021 gaccattccg tgaggagccc ctccgtcatg gtcagcccca gctggggcat gagcaccggg
    10081 atggccatgc tacgcctccc tgtggaggac ccgcaggacc gcccgctcga tgtcggacag
    10141 gttcgcggcc acgtacttct ccaggggcgg gctgaagggt atgggcacgt ccttggcccc
    10201 gacccgaagc accggggcgt ccaggtggta gaaggcctcg gcctggaccc gggcggcgat
    10261 ctccgccccg agcccgccgt tcacgacgcc ctcgtggacc accacgcagc ggtgggtttt
    10321 ccgcacggag tccaggatgg tcgcggtatc catgggcttc agggtcagga ggtccacgat
    10381 ctccacggag atacccttgg cctccagggc cttggcggcc tcctcgcact tctggaccat
    10441 gagggaatag gtgacgacgg tgacgtccgt gcccgggcgg cggatctccg ccttcccgag
    10501 gggggtcagg tactcgcctt cggggacggg gcccttctgg gggtagagga ccttgtgctc
    10561 gaagaagatg accgggtcgt tctgccggat ggacgatttc aggaggccct tggccgtggc
    10621 ggggctggag ggggccacca ggatcaggcc gggcacgttc atcagccagc cctccacgga
    10681 ctggctgtgc tgggccgccg cctggttgat gatcccgtcc ggggcgcgga ccaccagggg
    10741 gatgttggcc tggccgccga acatgtagcg gttcttggct atctggttga cgagctcgtc
    10801 gtaggctacg gagatgaaat ccgcgaagtg catgtccgcc acggggcggg tcccggtgag
    10861 ggccgcgccc agggccgcgc cgatgatggc ggtctcgctg atgggcacgt cccggatccg
    10921 gtccccgaac tcctcgggca ggcccttgaa ctggccgaag atcccgccct gccggacgat
    10981 gtcttcgccg tacacgaaga ccgtggggtc ccggcgcatc tcctccttca tggcctccag
    11041 ggtcgcgtcg ctgaaagtaa tggtcctcat cgtacagcct ccgggaattc gctgggggag
    11101 aaggagaaga ggtcgtccag ggcgtcctcg ggatccggaa gggggctgga ctcggcgaag
    11161 gccacggcct cgtcgatctc ccgctccgcc tcggcgcgca gggccgagag ggtcgcctcg
    11221 tcggcggcct tcttcccggc caggaaggcg aagtaccgct cgatgggatc gttctccctc
    11281 tgctgggcct ggacctcctc ccgggtccgg tacttctcgg ggtccccgac gtagtggccc
    11341 ttgatccggt aggtcagaca ctccaggatc gcggggccct tgcccttccg ggcgttctcg
    11401 atcagggtgg tggcggcgtc gtagacctcc acgacgttgt tgccgtccac cgtgtaaccc
    11461 tcgatcccgt agcccttggc ccggtccgcc acccgggcgg aggagaggtg gtaccccgtg
    11521 ggggtcgtgg aggcgtaccg gttgttctcg ttgacgaaga ggaccgggag cttccagatg
    11581 gaggccaggt tcagggcctc gtggaaggtg ccccggttgg atgccccgtc cccgaagaag
    11641 cagaccgcca cctggtcggt cttcttgatc tgggcggaca tgcccgcgcc cacggcgatg
    11701 ttgtagccgc cgcccacgac tccgttggcc cccagcatcc ccaccgagaa gtcggcgata
    11761 tgcatggagc cgcccttgcc cttgcagaac ccggtcttct tcccgaagag ctccgccatc
    11821 atgcgctttg tgtccgcccc cttggccagg acgtggccgt ggccccggtg ggtggaggtg
    11881 atccagtcgt ccttgcgcag gttggccatg accccggtgg cgatggcctc ctccccgatg
    11941 tacaggtgca cgaatccggg gatcttcttg ttcatgaaca gcatttctgc ccgggtctcg
    12001 aaggctcgta tccgaaccat ggtgcgcagg agtccgacct tgtccgaggc cggaagctcc
    12061 gcgaaacggt tcgggaattc tttgatcctt gacggcattt cgctcctcct ggcttcaact
    12121 atgcaataac cgtgccgaaa aaaacgtttt tcggtatttt tatttgcctc taccgcaagg
    12181 acttattccg gacccgggtc gcctctttcc ggttgcctgt ctcagaatgt tgcactatac
    12241 tgcgatttac tgtcacggaa tggagcagcc gatatgatac agtggaccca caccagccgg
    12301 gtcgtggacg tggacttcga ggtggacgga gcggacctgt tcaagagttc acccgccacc
    12361 atcatgaaga caatcatcga ttccatctcc gacgggatca tcatcctgga caagtccggt
    12421 accgtcctgt atatgaacca ggaaggggcc cggatcctgg gattgaacca ggagggggtg
    12481 atcgggaaga acgtgtcgga catcgtggat ttccggcccg tgatcctgga ggtcctggag
    12541 agcggcaagg gctacatcga gaaggaattc atcatccaca gccccagtcg gggccagctc
    12601 cacttcatca agtccgcggt gatcctccac gacaaggcgg ggaatatgat cggggtcatc
    12661 gacaccttcc ggcaggtgga gaaggtccac accatgatcg cccggatgac cggggccaag
    12721 gccaagttca ccttccagga tatcgtgggg gaggatccct ccttccggga ggcggtcaac
    12781 ctctcccgga tggccgccca gagccgggcc aacgtcctga tcgaggggga gagcgggacg
    12841 ggcaaggaga tgttcgccca cgccatccac cacgccagcg accgcagcgc gggtcctctc
    12901 atcatcgtga actgcgcggc cctgccgagg tccctggtgg agagcgagct cttcggctac
    12961 gagtccgggt ccttcacggg ggcccggaag gaaggcttcg ccgggaagtt cgagcaggcc
    13021 aacatgggga ccctcttcct ggacgagatc ggggagctgc ccctggacat gcaggccaag
    13081 ctcctccggg tcctccagga ccggaccttc tcccggatcg gcgggaacaa gagcatcacc
    13141 gtggacaccc gggtcatcgc ggcgaccaac aagaacctcc tttcggagat ccggaacaac
    13201 gccttccggg aggacctgta ctaccgcctg aacgtagtga acatccactt ccccagcctc
    13261 cgggagcggc ccggggacat tgccctcctg gccgcccact tcgcccagaa gagcgccgcg
    13321 tactacggca agccagtcta cgagatcgat tccgaggtcc tgcgcctctt ccggggctac
    13381 ccctggcccg ggaacgtccg ggagctggag aacgtgatcg agcgggccat gatcgtggcc
    13441 aagggccatc gcatcacccc cgcggacctc ccgccccacg tcgccgccat ggagtgcgcc
    13501 cccggcacga agccttccgc gtgcagggac caggaggtcc tgaacctgga ccgggtggag
    13561 cgggacgaga tccagagggc cctgcgggtc tgcgagggga acatctcctc ctgcgccaag
    13621 ctcctgggca tcagccggaa caccctgtac cggaagatga aaaaatacga gatctgagcg
    13681 atttcaaggc cgagaaacca tagatccaac aagatcaaca agatggatca gggtcagcaa
    13741 gacagagcat cggaggaaag atccacttat atttattgat cttgtaaatc gttcttgcat
    13801 cttgtccatc atgttggaac tttggttcca aggtcgggtt ttctccgtgt cctccgcgct
    13861 ctccgtggtg agtccagaaa gaatcggccg gccccgaggg accggccgtt gcattttccg
    13921 gaaaggccgt tacggcgtgt agagccccga ggccacgggc agccagaggg agatctgggg
    13981 gatcagggtc aggaagatca gggccaggac cgcggacacc accatcggga tgacgaactt
    14041 caccatccgg tcgttgatgt tgctgccgat caggttggcg gtcacgtaga ggttgaccgc
    14101 cacgggcggg gtgatctggc cgatggccag ggccaccacg aagacgatcc cgaaatacag
    14161 ggggtcgatg cccagggcca gcagggcggg caggatgatg ggcatgaaga cgtaggtgat
    14221 ggagatggcg tccaggaaca tcccggcgat caggatcacc acgttgcaga gcatcacgaa
    14281 gaccagggga ttcttcgtga ggctggacac caggttggcc gccgcgtcga tcacccccag
    14341 ggtcgaggcc gcccaggtga agatccccgc catggacacc acgaacatca ccaccgcgga
    14401 gccctggacc gtgtcgatca gggccttcat gaggacatcc aggttgatgg tcttgtagat
    14461 gaacaggccc acgaagaggg cgtagaagac ccccatgatg gcggactccg tgggggtcgc
    14521 gatgccggcg tagatggagc ccaggatgat gaccggggcc aggagggccc agaaggattc
    14581 caggaaggcc gcgaggatct ccttcggggt tcccgaggcc acggagccgc ggaacccgtg
    14641 tttccgggcg cccagataca ccgccacgat caggaagatg cccaccacga tgcccgggag
    14701 gatgccgccc aggaagaggg ctcccacgga cacgttggtc aggttcccgt acacgatgaa
    14761 ggcgatggac gggggaatga tgatggacag gcccgcgccc gccgcgatcg tggaagccgc
    14821 gtagtacttg tcgtagccct gcttgaccat gggggcgatg aagaccatgc ccagggccgc
    14881 ggcggtggcc gggccggagc cggagacggc gccccagaag atacaggtga tgacccccgc
    14941 gatcgccagg cccccggtcc gcttgcccgt aagcaggatc acgaacttgg agatgccgtc
    15001 cacgatccgg gatttctcca tgaggtttcc cgccaggatg aagaagggga tcgcgatgag
    15061 ggggaacttg gcgatgttgg cctcgatgtt gcgggagacc atggccacgc ccaggtcgaa
    15121 gttcaggatg gccccgatgc ccgcggctcc cagggctatg ccgatggggg cgccgaaggc
    15181 gatgagcagg acgaagagtc cgaagacgat gagtgccgcc atcacgcgcc tccctcggcc
    15241 ttgtcggcct tgtttcgggg gaaggtggcg cggaggatcc gccagatcac catgcccgag
    15301 aagatcgggg ttcccaggct gtagatccag gtggggatcc ccagggcctc cgaggtggtc
    15361 ctgtagaagg tcatattctt ccagatctcc tggcaggagt tcacgatgac gaaaccgaag
    15421 atcaggatgc cgatcacccc ggcgcccacg gtggcggctt tctgcagctt cggcccgaag
    15481 cggtcgaaga ggttcgtcat ccggaggttg gcccccttgc ggaaggccat ggctatgccg
    15541 atcagggtga tccagacgaa gaggttcacg gtgatctctt ccgtgaaggc gatgctcctc
    15601 ttgaacacgt agcggttgat cacgttgagg aaggtcagga ccgacatgcc ggccaggagc
    15661 accgccccga tcacctcttc cgcgtggtat ttcttctttt ccatgggttg tcccggtcct
    15721 cctggattgg atctcgtgat ggtgcgggcg gctcgccgga tgtcgtagaa accgcaaggg
    15781 gtgtcccacg agcggacggc tcatggggca ccccgacacc ggtcattacc gcggcacctt
    15841 cggcgaacat gccgccttga agcggtccgg cgctccggac cgctccttcc ggacggtctt
    15901 acttattcat gtcctggacg gccaggtcgt agatgttctt gccgatctgg gggatccact
    15961 tgtcgtagat cggcttggtc gcgtccttga aggcctgcat ctgggcgggg gtcaggttgg
    16021 tgatgaccat cccgttgtcc acggccagct tgaagggctc gatcggcatc tcggtgacct
    16081 tgtacttggt cttgaggatg tccagggact tggtcccgtc caggtgggcg cgggccaggg
    16141 ccttctggta tccggcggcc tcgaccgcgg cttcctcgat ggccttctgg atgtccttgg
    16201 ggaaggagtt ccagaccttc atgttcacgc cgaacacgag ggggtcgagg acgtagttcc
    16261 aattggtcat gtacttgtgg tattcccaga tcttgacgac cgtgaggacg gacttggggt
    16321 tctcctggcc gtccacgacc ttctgctgga agccggtcac cgcgtccgcc cagttcatgt
    16381 tcaccgggtc ggcaccgaac tgcttgaaga tgtccacgaa gatcggggaa ccgaccacac
    16441 ggagcttgag gcccgccagg tcctggggag tcttgatctc ccgcttgctg ttcgtcacct
    16501 gccggaagcc gttctcgccc caggccagga acttgatgcc gccgcgctcg aactccttca
    16561 cgatggcggc gcccgccttg ccggcctgga tcttgtccac gttctcgtag gttcccagga
    16621 agaagggcag gttgaagagg ttgaaggccc ggatgaccgt ggacgcgttg atcgtggact
    16681 ccacgacgaa gtcgatggac ccgtccgcca cggcctggta ccagttggtc tgcttgcccg
    16741 ccaggaggga ggagccgaaa tacggcttca cgttgacccg gccgccggtc ttttccttca
    16801 cgaggtccgc ccacctctgg gcgccgatgc cccagtcgaa ggccgggccc acgttcacct
    16861 gcatggtgta ctcggctttc ggcttgaagg agccctgggc cgcgaccatg ccggcggcca
    16921 gggagaagag cgccagcgcc gcgagcacgg tcaggaatct ggttcgtttc atgatagcct
    16981 ccatcggagt gtgtaaccta ttttcccccg aaggtcaaaa aatttcaggg aacaggtcgt
    17041 tgaaagtatt aatgcaataa gcatgccatg ccggcttggg tgccgaggcg gatgtatttc
    17101 ctttccgggc tagggccgga cggatcgcat tgtagggcac gaccccgcgg gactgtcccg
    17161 ttgtgtgtca tctctgcctt gagatgctca aaaacgggac aggattgtgt cactccgcga
    17221 ggcaccctcc cgggtatcct gaaaggaacc gcttgcggcg atcccggatc cgtggctata
    17281 atcgggacca tcatcccgga cgaggtggag cgatggaaga caagacgtac gacgtggcga
    17341 ttctaggggc gggacccgga gggtacgtgg cggcgatccg agcccggcag ctcgggctct
    17401 ccgcggtggt catcgagaag gacaagcccg ggggagtctg cctgaacatc ggctgcatcc
    17461 cctccaaggc cctgatccac caggcccagg tcttctcgtc cgccgcgggg ctccgggagc
    17521 tgggggcgac ggtggacctg tccggcttcg attacgggaa agttttccgg aaatcccgca
    17581 tggctgcgga ccgcctgtcc aagggggtgg agttcctcct gcggaagaac aaggtcgact
    17641 tcgtcgcggg cacgggagcc ttcaagtccg cggaccggct tatcgtggac ggaaagaccg
    17701 agatcgcctt caagaacgcc atcgtggcta cggggtcccg gccccgggag atcccgggct
    17761 tcgccttcga cgggaaggtc gtcctctcct ccacggacgc cctgatgctg gagaagcttc
    17821 cccggcgcct cgtgatcctg ggggccgggg ccatcggcat ggagttcgcc tacgtcatga
    17881 actccttcgg ggtcgaggtc accctggtgg agatgctgga caccatcctg cccttggaag
    17941 actccgagac cgtggaggtg gtgcgccggg acttcgtcaa gaagggggtc aagatcctga
    18001 ccgggacccg ggcggagggc cttgagagga aaccggaagg ggccgtcgtc cgcctgtccg
    18061 ggaaggacgg ggccaagacc gaggtcgagg cggaccaggt cctggtggcc gtgggccggg
    18121 cccccaacac ggaaaacctg ggcctggagg ccctgggcat ccggaccgaa aagggcttca
    18181 tccccgtggg ggactacggg cagaccgccg tcccgagtat ctacgccatc ggggacgtgg
    18241 tcgccacgcc cctcctggcc cacgtcgcct ccaaggaggg ggagatcgcg gtggagagga
    18301 tggccggcaa ggatcccgag cgccgggtgg ccctggacga gatcccctcg gcggtctact
    18361 gcgagcccca ggtcgggagc ttcggcctca ccgagcggga ggccgtcgag cggggcgtcc
    18421 ggttccagaa ggccgtcttc ccctaccggg ggatcggcaa ggccgtggcc gtcgaggcgc
    18481 tcgacgggat cgtcaagatc ctccacgatc cccagaccgg ggagatcctg ggcgcccacg
    18541 tcgccggggc ggaggccacg gagctcgtcc acgagctttt gctggccaag aagtccgagc
    18601 tgaccgtcca ggacatcgcg gggatgatcc acgcccatcc gaccctctcg gagggggtga
    18661 tggaggccgc ccgcctggcc gagggctggg ccatccacat ttaggagcgg cggactcccg
    18721 gccggaccgc ctcggtctag gccggctccg ccgtccggca tgccgccctc agcggttcag
    18781 ctcgttcagc tttcttaggg ccccgtccag gtggcggcgc agggcgtcct gcgccgccgc
    18841 cggatcccgg gccgcgatcg cctccaccag gcggcgatgc tcgtccagga tcttcccgga
    18901 ccatcccccc agggccagga gcttggaccg gctcaggacg atgtacttct ccatggtcga
    18961 cgacagtccc tcgtacatcc ggctcagcac ggggttgtgg gcggcgtcca ggaccagggt
    19021 atggaggagg gtgtcccaga ccgcccccga ggagaggcct ccgtcggggt tgtcctgctc
    19081 ggcttccagg tgggccacgc agtccctcat ccgggccagc tcggcgtccg tcctgcgctc
    19141 cgcggccagc cccgccgcgg gcacctcgat gacaagccgc atctccatca ggtaggacag
    19201 gaggtcctcg ggccagatgg acaggaactt caggctggcc gcgaagtcct cggtccccgg
    19261 gggtgtgatg aaggccccct ggcgttcccg gatctccagg aagcccaggg cttccagggt
    19321 gacgatggcc tcccggagga gattccggct gacccccagg ctctccgcca gctcccgctc
    19381 cgggggcagg cgtccttcgg gaaagcgtcc gggctcccgg atcaggttcg tcagggtgcg
    19441 caccagctcg gcccgtttct tttccatgtt gacggcagtg tataccggtg ctatgattcc
    19501 gtcaatgctg gtactaccaa ggaagtacca agaacaagga gaccggaatg agaaaagtcg
    19561 cggcgatcat ctccctggcg tgcctggccg tttccgcctt cgccctggac atcaagcttg
    19621 cccacgtggt gaacgagcag gattccttcc acctggcggc cgtcaagttc aaggagctga
    19681 ccgagaagta caccaacggt gcggtcaagg tcacgatctt cccgaacgcc accctggggg
    19741 acgagcgcac cctcctggag cggatgaaga tggggatcgt ggacgcgggg atcatcacga
    19801 acgggccgat catcaacttc gtgccccgct tcggggtcat cgacctgccc ttcctcttcc
    19861 gcagccccga gcacgcctac aaggtcctgg acggttccat cggacagagc ctcttcgcgg
    19921 acctggaggc ccagggctgg aagggcctgg cctgggcgga gcgcggcttc cggaacctga
    19981 cgaacaaccg caagcccgtc cagtccccca aggacatgga gggcctcaag atccgggtca
    20041 tgcagaaccc cgtgtacgtg gactccttca aggccctggg cgccaacgcg gtcccgatgg
    20101 cctggaccga ggccctgacg gccctccagc agaagaccat cgacggccag gagaaccccc
    20161 tgaacgtgat cgtggccttc aagctggacg agagccagaa gtacatgtcc atcacccgcc
    20221 acgcctacgc cccggccccc atcctgatga gcatgatgac ctggaagaag ctctccgccc
    20281 cccagcagga ggcggtcctg aaggcggccc gggaagcggc ccagttcgag cgggacttca
    20341 acaactccag cgaggcccag tggatgaagg acctggccgc caagggcatg gtcatcagca
    20401 ccccggacct caagcccttc ctggattccg tgaagcccgt ctatgactcc tacgcggaca
    20461 agttcggcaa ggacctgatc aactccatcc tggccgtcaa gtaaggggcg ggcatgtctt
    20521 ccgcaaccga gcggaggggg gcgcaagccc ccttccttca tgccgcaagc gacgcggtca
    20581 acctgtggag cgaacgcttc ctcttcgtcc tgatgctggc catggtcggg gtcacgacgg
    20641 cgcaggtcgt cttccgcttc ttcttccagg ccctcacctg gtccgaggag ctctcctgct
    20701 tcctcctggt cctggcctcc ctggtgggat ccgccgtcgc cttcaagaag ggcagccaca
    20761 tcgccgtcac cttcatcgcg gatcgcctgc ccgagacggg ccgcaggctg ctggccaccc
    20821 tggtgcacct gctcgggctg gtcttcttcc tgatcgtggc gtactacgga gccgtcctca
    20881 tgaagtccga ggcgggccag acgaccccgg ccctgcagat ctcgatgaag tgggtatacc
    20941 tgatgtaccc cgtcgccggc gcggccacgg cattgcacct tctggacggc atgttcaggg
    21001 tctggacggg aggcgagtca tgatgggctg gatcatcttc ggctccttct tcctcctgat
    21061 gctgatcgga gtgcccatcg gcctggctat cggcctggcc tccctgatcg tcttcctggc
    21121 cagcggcttc cccctgcaga tggtccccca gaccctgctg gagggcaaca actccttcgc
    21181 cctggtggcg gtgcccttct tcatcctggc cggggacctc ctggccaagg ggggcatctc
    21241 cgagcgcatc gtctccttcg cggaggcttc cctgggcaag cgaaggggcg ggttgtccat
    21301 cgtgtccgta ctggcctcca tgttcatcgc cgccatctcc ggctccgggg ccgcgaccac
    21361 cgcggccgtg ggctccgccc tcctgccgga cctcaaggag aagaagtacg acgtggactt
    21421 ctccgcggcc ctgatcgcgt cggcggggac catcggcgtg gtcatccccc ccagcgtccc
    21481 catggtcctg tacgcggtga tcgccgggga gtccgtggcc aaactcttca tgggagggtt
    21541 catacccggg accctgatgg gcgcgggact gatcgcctac gccgtgtggt actcgaaccg
    21601 gcggggatac ccggccagtg agaagctacc cccgcgcgcc gtcgcccggc ggttcttcga
    21661 ttccctctgg ggcctggcga ccccggtgat catcctcggg ggcatcttca ccggctactt
    21721 caccccctcg gaagcggccg ccatggccgt ggactacacg ctgctcatcg cctggctcgt
    21781 gtaccgcacc atgaccttcc ggcggttcta cgaactggtg gtgggagcgg gggtcacctc
    21841 cgccctgatc atgttcatca tcggcacggc gaagattttc ggctggggcc ttgccttcta
    21901 cgagatcccc
//
LOCUS       DAOTEI010000039        21773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1589_length_21773_cov_5.106870, whole genome shotgun sequence.
ACCESSION   DAOTEI010000039 DAOTEI010000000
VERSION     DAOTEI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 21773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21773
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1589_length_21773_cov_5.106870"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1671)
                     /gene="hutH"
                     /locus_tag="P5313_05690"
     CDS             complement(160..1671)
                     /gene="hutH"
                     /locus_tag="P5313_05690"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRY79891.1"
                     /translation="MEDILLDGESLTLDQVELVARRGARAALADSAIPKMEASRAVVD
                     RCVSERLVRYGITTGFGKFSDVVISPENNAALQRNLIMSHACGVGDPFPAEVVRAILL
                     LRANALAVGSSGARVSTVRALLDLLNADVVPVIPEKGSLGSSGDLAPLAHMALVLCGM
                     GEAFHGGRRLPGAEALRLAGLEPVELSAKEGLALINGTQAMTAVAALACADAARLVGL
                     ADGVAALSHQALRGITDAYDGRIHELRRQDGQIASAAALRDLLSGSGLTTRQGQERVQ
                     DAYALRCAPQVHGASRDAIAYVTAIVGRELNAATDNPLIFPDGSGYGDILSGGNFHGQ
                     PVALAMDFLAIALAELANLSERRTERLVNPALSNGLPAFLVKEGGVNSGFMILQYVAA
                     ALVSENKVLAHPASVDSIPSSANQEDHVSMGTIAARKAREVLGNLQQVLGIEWLAACQ
                     AVDLLDGAEKLSPATRKLYDRLRAEVPFVDRDRVMYPDLEKARRLVAAGPEAG"
     gene            1817..2440
                     /locus_tag="P5313_05695"
     CDS             1817..2440
                     /locus_tag="P5313_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRY79892.1"
                     /translation="MKSKKNHSSRLIFLAACLAFLPSVSLSAAQQDPALPPPGTPNAA
                     LILPPRNFYDPEFFAVRDALSAAGVRVRIFCNAEGDAVGSQGGKVRPDAPLAELRPAE
                     FDLVCVIGGTGTLNYLYHDEALGEKLREARDQGKRVAGICAGATTLAKFGVLSGRTAT
                     GANRGVFVRAGVAYSFEKVVVDGPFITAQGDYHDLFAAALVRELTGG"
     gene            2529..2612
                     /locus_tag="P5313_05700"
     tRNA            2529..2612
                     /locus_tag="P5313_05700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2563..2565,aa:Leu,seq:cag)
     gene            complement(3092..3460)
                     /locus_tag="P5313_05705"
     CDS             complement(3092..3460)
                     /locus_tag="P5313_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79893.1"
                     /translation="MRKPLSLLLVEDEALIALNLKIGLERAGFAVNAVLATGEKAVER
                     MDQEPADLIVMDIRLAGTMTGIEAARRIRNRHPVPIIFMTGFTREDYGPEIDELGPSL
                     LLNKPVTSESLANAIEEIRG"
     gene            complement(3465..5384)
                     /locus_tag="P5313_05710"
     CDS             complement(3465..5384)
                     /locus_tag="P5313_05710"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HRY79894.1"
                     /translation="MAYKLADLIDIEQFQHLQDRLNAIYSFPSAIIDNDGVILTATAW
                     QDACTKFHRLNPECEVECLKSDRYILDHLSEANPAVSYRCPHGLVDNATPIIIEGIHY
                     GNFFTGQFFLEPPDLDFFARQARRYGFDEAAYLEAIRKVPIWTRDQLEHYLYFIKGLI
                     EIITESGLKKLREIEARKKIEASEARADVVIQKMLDGFWMVAADSGRILDVNDSMCAM
                     LGYTREDLLALSVADIDAIDDSDEVARRMEKIASEGSALFESRFRRKDGSLLDVDISV
                     TYIRDQNLFFGFHHDNTARKAAEAASRESEDRYRTLFEQSPDGVVILDPATARPLEFN
                     EQVCRQLGYGREEFARLSLADIEVIESLEESLRHIKKIIETGHDEFETKQRTKQGEIL
                     DVLVIAQVIRSGEASIFHCIWRDITERKRVDARLKKSLAEKETLLRELYHRTKNNMNV
                     IGSMLELQAAYSGVPAVEAMARDTERRIRAMSLVHQMLYQSKDLSHVSLRQYINELLS
                     LLLHGYSTETRDIRLETEIEDIEAVIDTAIPCGLILNELVSNCFKHAFPEGRPGSVRI
                     RLSRVDPLTLELVFSDDGVGVAPEFEYRKQNSLGFQTIIGLAEHQLQGHAGFEIDNGV
                     TWRIRFSDQLYAARV"
     gene            5645..6403
                     /gene="ppk2"
                     /locus_tag="P5313_05715"
                     /pseudo
     CDS             5645..6403
                     /gene="ppk2"
                     /locus_tag="P5313_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
     gene            complement(7019..7987)
                     /locus_tag="P5313_05720"
     CDS             complement(7019..7987)
                     /locus_tag="P5313_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HRY79895.1"
                     /translation="MTRTRDESAPDAPSLRAERILSLFLLLAGGRLHSAPDLARRFGV
                     SARTLYRDLDLLSAWGCPIESIPGREGGFRILPGYALDRSVLDRSELAAASAALAGVE
                     EAVGGRGLERAAAKIDALLGRPRSRRSSWLRISLAPGRDERALVELLRDAIEGRRTVQ
                     FGYRDAEGRRTDRTVEPAAVVYLWEGWYLWAYCRLRRGWRHFKLARIERPRTRIERFE
                     PRPEPSEEAWRGGGGTRTDLRIRVAPAALPRFEEDLGRGFGTPDGRGGRIAELRMPAG
                     EWLFDFLLGFGDGIEVLGPPELRAEFAARARRVAELYETPGNSDKP"
     gene            8096..8593
                     /locus_tag="P5313_05725"
     CDS             8096..8593
                     /locus_tag="P5313_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY79896.1"
                     /translation="MSKNISFFFDYADGEFDGESFNGPSLMATLDCLTAQAAAGTDTW
                     EGYSAWSVAIHVAYYKYYFVRAIRGQDKAGVFPYAKDQHGFGDPPEVTAEAWVEMRAY
                     LRKIHRAVMDALVELGPERFAETMPRWDMPYGKAVAWLLNHDAYHAAQIRNMGVPGLR
                     EKRPT"
     gene            complement(8610..9143)
                     /locus_tag="P5313_05730"
     CDS             complement(8610..9143)
                     /locus_tag="P5313_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRY79897.1"
                     /translation="MDAISILKHEHEAILFGLGILDRAAARASTGDAAARADLPELVR
                     FLSGFADTCHHGKEEGILFPALEAAGMPREGGPLGVMLEEHRLGRSFIRDMGGALGSG
                     DYAAFARAASGYSALLRAHIEKEDGILFPAGAEALGRSRLEALGGDFDRFEETVMGPG
                     EHERLHSLLEEFAGRYR"
     gene            complement(9153..10139)
                     /locus_tag="P5313_05735"
     CDS             complement(9153..10139)
                     /locus_tag="P5313_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HRY79898.1"
                     /translation="MAKRAVIEIDEEKCDGCGLCAGGCHEGALRIIDGKARLVGESLC
                     DGLGACVGECPRGALRTVEREVEDYDERRVIENILPKGMNTVAAHLDHLRVHGQDTWL
                     AQARAVLAEKGLAVPEPEPALPGCGASFPEPRAAAGTRGSSLLRPAAPASFAAPRIDN
                     GISGGCPGSAARSFASGAGTPASTPARPKPALGPSGAGASGSLASRLEQWPVQLHLVN
                     PRAPYFRGADLLIAADCTAFACGAFHQALLAGRRLVIACPKLDHGREVYLDKIRALID
                     ESEVASVTVAIMEVPCCGGLSRLVQEAAASASRAVPISTVVVGIEGGGLTWM"
     gene            complement(10226..10777)
                     /locus_tag="P5313_05740"
     CDS             complement(10226..10777)
                     /locus_tag="P5313_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY79899.1"
                     /translation="MNGIIHLSDASLIAVHALAALASEPDRRVLGKDLAASMGASENH
                     LAKVMQRLVRAELVLSVKGPHGGFRLARDPGAVTFREAIEAVDGPLTGDFCPFSPDHC
                     DPSACIFGHELSRQAEGLVAYLGRRTIADIVRDGVPRFLSGPGDGGRKKPDGSPKGRR
                     AAAGSRIAGANPEPARPDRVRRG"
     gene            complement(10856..11083)
                     /locus_tag="P5313_05745"
     CDS             complement(10856..11083)
                     /locus_tag="P5313_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRY79900.1"
                     /translation="MLDDPGFLLRLANARMPFGRYAGTLLIDLPEAYVVWFARAGWPE
                     GEIGEQLAALYSIKENGQEGLLRPLVRLDER"
     gene            complement(11088..11861)
                     /locus_tag="P5313_05750"
     CDS             complement(11088..11861)
                     /locus_tag="P5313_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY79901.1"
                     /translation="MDLKVKGLRALVTGASSGLGFATARLLAAEGARVALNGRDPDKL
                     AKAAREIRAEGVPGDLSEAGVPEAVVARAAEALGGIDLLLVNAGGPPPGSFESFDDGA
                     WAKAVDLCFLSAVRLIRAALPHLRRSGSASVLTVTSVSVKQPIPNLILSNSVRAATVG
                     LTKSLALELGGQGIRFNSILPGWTATERATKLVASSAAAKGTTVEAELAERARDFPLG
                     RMATPEEFANVAVFLLSPAASYVTGSMIAVDGGAYKGTL"
     gene            complement(12099..12788)
                     /locus_tag="P5313_05755"
     CDS             complement(12099..12788)
                     /locus_tag="P5313_05755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRY79902.1"
                     /translation="MKKLILAVLVASSLLLSCKGQDKPAEGEAPKASKKDVSYAFGVA
                     IGNSLKEAYVSLDYSSFLKGIKDVLDKDKPAIDMEKAQELIQAAVMEAQEKKNAENAE
                     KEKTFLAENGKKDGVKTTESGLQYVVLSEGSGEKPKATDSVKVHYTGTFTDGKTFDSS
                     VERGEPAEFVVEEVIPGWVEALQLMPVGSKYKLFIPSSLAYGEEGAGGVIPPFSTLVF
                     EVELLSIEKAK"
     gene            complement(12946..13812)
                     /locus_tag="P5313_05760"
     CDS             complement(12946..13812)
                     /locus_tag="P5313_05760"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRY79903.1"
                     /translation="MERKRRILIAAAALFAVLFAARLAWELTRDRTAPSPFGGFGAGE
                     MVQSSVPPQERRNYASAKIAVDQAPSGAQVVEQKYERVSQIESRTGHWDSDLAAVKLA
                     IEGAKALVQRENSYGLPGARVLSISLGVIPDAFDPTVETLKAIGDLRSANVIKTDRTS
                     DFRALEARRLSLEKTRDGLVGLRDSGASLADRIDLETRILEIEGQIQELGVSLGDFSE
                     LNSFCTIHFTLREVPSGARGSLLLGAVLSALGWTVLTYLGLGACVLAALGAAVLGLKL
                     ADRLKEGKARRP"
     gene            complement(13833..14645)
                     /locus_tag="P5313_05765"
     CDS             complement(13833..14645)
                     /locus_tag="P5313_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRY79904.1"
                     /translation="MFDTFARLPHTVQALLATLFTWGVTALGAGMVFFFKKIDRRVLD
                     AMLGFAGGVMTAASFWSLLAPGIEMAESMGMVPWVPAVIGFLAGGLFLKLVDKILPHL
                     HIEAKLEDAEGPKTSWQRSILLVFAITLHNIPEGLAVGVAFGALAAGIPGASLAGAAA
                     LALGIGLQNFPEGAAVSIPLRREGMSRLKSFWYGQASGVVEPIAGFLGAALVVAAQPL
                     LPYALSFAAGAMIYVVVEEVIPESQGSGNSDISTLGALGGFALMMLLDVALG"
     gene            complement(14700..15776)
                     /locus_tag="P5313_05770"
     CDS             complement(14700..15776)
                     /locus_tag="P5313_05770"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRY79905.1"
                     /translation="MFEEIRRPTLVVDTGKVRENVLRMKGRADRNGTAFRPHLKTHQS
                     RVIGRLFRDLGVRTGTVSSVEMAKLFADDGWDDLTIAFPANLREIGEMDELAGRIRLG
                     LLADNPETIRALAAGLSRPVDLWIKIDVGSRRCGIRAEDREGLRAVSEAVRAAPRLRL
                     AGVLTHDSRTYGVRGREAVAALFREGRDALLAAKAFLASEGWPDLRASFGDTPSMSLV
                     EDWSGLDEVRPGNFAYYDLQQYVAGSCSPGDIAAAVACPVVGVYPDRGEAVVYGGAVH
                     LSKQSQTLPEGGTSYGAVFEVRDGRWTGFREGAGVRTVTQEHGIVRMGPEDLASTRIG
                     DLVFLCPVHSCLAADLLRPNTVYL"
     gene            complement(15831..16334)
                     /locus_tag="P5313_05775"
     CDS             complement(15831..16334)
                     /locus_tag="P5313_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY79906.1"
                     /translation="MIDTASLQKYSLFGGMIPDQVERIRPLLLPSSYSEGQSVLREGD
                     PNDSVYFILDGRVQIVKGTRVLTELGEGETFGEMELLDVQPSAATVRALGPLRVAAIS
                     NRAVRQIYCMDPKIFALLMMNLARDLSRKLRRMDELAAALSDPAARDFSSPAPPRDLG
                     PAGGLDC"
     gene            16503..17204
                     /locus_tag="P5313_05780"
     CDS             16503..17204
                     /locus_tag="P5313_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY79907.1"
                     /translation="MKRKAGFPEDLLFGAAPAGTAPAEGPTAAAAGPASPGDPSPEEA
                     LLRAYLRVLALKRLYRQGWLKRGVPEERCESVADHSFGTAVLALFLAGREPFRDADPG
                     RTCRMALAHELGEVYAGDITPVDGISKEEKYRMERESLLRVLEGAPGSGEILALWEEF
                     EEGASPEARLLRRLDRLEMGIQAAVYRAEGFPRMEEFLESARKAAGEGLPGEVLAMAV
                     NRPSSSADPAGPRSP"
     gene            complement(17155..18138)
                     /locus_tag="P5313_05785"
     CDS             complement(17155..18138)
                     /locus_tag="P5313_05785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRY79908.1"
                     /translation="MTAPFDVLNPELAMESVREAFGLDLKGIWVPYPSYVNRVYGLRS
                     ADGRDFVAKFYRPGRWTAEAIREEHAFLAELAAAEIPVAAPLPDSEGDTLPVLALEAP
                     DGGDVLYPFALFPRRGGRLFDAESDEDWLRLGRLAGRVHAVGALRSAERRISFRPGLL
                     SAYVEELEREGMPHPECRAEFREAVEGAREILDPLLAEARLLRIHGDFHRGNILDRPG
                     EGLLLMDFDDMVMGPAVQDLWLLLPGPSDECRREFALILEGYEEFRPFDSAERALIEP
                     LRFLRMVHYLAWQARQRGDAGFLRHFPDWGGRAFWIKETEDLRDQLRRMDG"
     gene            18253..19242
                     /locus_tag="P5313_05790"
     CDS             18253..19242
                     /locus_tag="P5313_05790"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY79909.1"
                     /translation="MRIFITGATGYIGSALALSLLEEGHEVAALARNPDRGGFLAARG
                     ARVVRGDLGDEGSLDRLCEGAEAGFHLAGWVKASGSPADYEEANVAGARRAFEAAVRA
                     GVRRVVFTSSAGVLGPALDGSPVHEDSPRRIGFLNDYERTKAQAEILVPGFLDRGLEI
                     VTVNPTRVYGCPVGPDLPPYTAMIDRYLRGKFLLIPGDGRSLGNYAFLDDVVEGHRAA
                     LARGVPGRRHLLGGHDLSYRDFFAALARASGVERRLPTAPAALLYAAAGLAGVAARLT
                     GGRPLLTPAWVKRFLYDWKVSSARAESELGYQITPLPVALARTVRGLRDRGGY"
     gene            19331..20446
                     /locus_tag="P5313_05795"
     CDS             19331..20446
                     /locus_tag="P5313_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRY79910.1"
                     /translation="MLDARLKRLAEILVNFSVEAKPGQKVLIQATNAETPFVTALVDA
                     VHDAGARPFVTLRDKTVDRALIRRCTAEQMEAQARYELARMREMDCFIGFTANRNLSA
                     WSDIPPEKLKLYNDILARKVHMECRVPDTRWVVLRYPTPGMAQLAGMSEEGFEDFYFS
                     VCTLDYARMSKAMDPLVELLARTDRVRIVSPGTDLSFSIKGLPPVKCDGRMNIPDGEV
                     YTAPVKESVNGVLAYNTPSEKDGFTFENVRLEFKDGKIVKATANDTDRINAILDTDPG
                     ARYVGEFALGVNPYITKAMKETLFDEKIAGSIHFTPGNSYDDCFNGNRSAVHWDMVLI
                     QTPEHGGGEIWMDGRLVRKDGRFVVPELECLNPENLK"
     gene            20460..21104
                     /locus_tag="P5313_05800"
     CDS             20460..21104
                     /locus_tag="P5313_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRY79911.1"
                     /translation="MRPRPRAPLLPTVIVFACVTVAAAFLAAGIPVACQSRPAPSASG
                     AGATAPVPAIPYSIHTERIREPSAADPSRMTVARVLRVVDGDTAVVEIPRPPAGLGTR
                     ETVRLIGVDAPELGRESRAADPGGPESAAAARALLEGRAVLLAFDRELRDRYGRVLAY
                     LYSPDGVCLNLELVRSGAARALLKYPFAFSSAFAAAEREARSAGRGLHSELYIP"
     gene            21169..>21773
                     /locus_tag="P5313_05805"
     CDS             21169..>21773
                     /locus_tag="P5313_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY79912.1"
                     /translation="MKRILAVAAILLAALSFAAAQETLSVYTTLEEPLAKAILEKFQA
                     ETGIRTSFVRLSGGEAIARMEAEAANPQASIWLGGVGLDHITAKDKGLTTAYKSRFAS
                     KTAAEFKDKDGFWIGLYVGPLTFVTNTKRAAELGLTPPKSWADLLKPEYKGQIRMANP
                     NTSGTAYNVITTLRYVYKGDEEKVFDYMKKLDANIDQYTRSG"
BASE COUNT         3540 a         7433 c         7427 g         3373 t
ORIGIN      
        1 gtacgatcga gcctgagtcc gaagtcgcgt ttcaccggca tgggggacct ccctggggga
       61 ttggtatgtt ctgggacggc ggtcggcgcg ccgaaacccc ggacatcata ccctgcccgg
      121 ggcggggact tcaattccgg ggtccgaaac cgggcaccgt caaccggctt ccggccccgc
      181 ggcgaccagc ctcctcgcct tttccagatc cggatacatc acccggtccc ggtccacgaa
      241 gggaacctcg gcgcggagcc tgtcgtatag cttccgggtg gcgggggaaa gcttttccgc
      301 cccgtccagg aggtccacag cctgacaggc cgccagccac tcgatcccca ggacctgctg
      361 gaggttcccg agcacctccc gggccttccg ggcggcgatc gtgcccatgg agacgtggtc
      421 ttcctggttg gcgctggagg gtatggagtc caccgaggcc gggtgggcca ggaccttgtt
      481 ctcgctgacc agggccgcgg ccacgtactg gaggatcatg aagcccgagt tgacccctcc
      541 ctccttcacc aggaaggccg ggagcccgtt ggacagggcc ggattcacca ggcgctcggt
      601 gcggcgctcc gagaggttgg ccagctccgc cagggcgatg gccaggaagt ccatggccag
      661 ggccacgggc tggccgtgga agttgcctcc cgagaggatg tccccgtagc ccgaaccgtc
      721 cgggaagatc agggggttgt ccgtggccgc gttgagctcc cggccgacaa tggccgttac
      781 gtatgcgatc gcgtcccggc tggccccgtg gacctggggc gcgcagcgca gggcataggc
      841 gtcctggacc cgctcctggc cctgccgggt cgtgaggccc gagcccgaaa ggaggtcccg
      901 gagggccgcg gcgctggcga tctggccgtc ctggcgccgg agctcgtgga tgcggccgtc
      961 gtaggcgtcc gtgatgcccc ggagggcctg gtggctcagg gccgccaccc cgtccgccag
     1021 cccgaccagg cgggcggcgt ccgcgcaggc cagggccgct accgcggtca tcgcctgggt
     1081 cccgtttatg agggcaaggc cctccttggc ggagagctcc accggttcga ggccggctag
     1141 cctgagggct tccgccccgg ggaggcgacg gcctccgtgg aaagcctccc ccatgccgca
     1201 gaggaccagg gccatgtggg ccaggggagc gaggtcaccg gaggagccca gggagccctt
     1261 ttccgggatc accgggacga catccgcgtt gagcaggtcc aggagggccc tcaccgtgga
     1321 aacccgggcc ccggagcttc ccaccgccag ggcgttggcc cgcaggagca ggatcgcccg
     1381 gaccacctcg gccgggaagg gatcccccac cccgcaggcg tgactcatga tcaggttgcg
     1441 ctggagggcc gcgttgttct ccggtgagat caccacgtcg ctgaacttcc cgaagcccgt
     1501 ggtgatgccg tagcggacca ggcgttcgga gacgcagcgg tccaccaccg cgcgggaggc
     1561 ttccatcttg ggtatcgccg agtccgccag ggcggcccgg gctcctcggc gggccacgag
     1621 ctccacctga tccagggtga ggctttcgcc gtccagcagg atatcttcca tggttgtctt
     1681 cgcctttcgt ggtttcgggg gacctgcact atagcacggg gcgggggatt ttggcaggag
     1741 gtattccgtg tggtatactc cgagccgtcg cggtacatat atcgtgctaa aataatcgat
     1801 tccgacagga gtactcatga agtccaaaaa aaaccattcc agccgtttga tcttcctggc
     1861 cgcctgcctc gccttcctcc cctcggtttc cctttccgcg gcccaacagg atccggccct
     1921 cccgcccccc gggaccccga atgccgcctt gatcctgccg cctcgcaatt tctacgatcc
     1981 ggaatttttc gccgttcggg acgccctgtc cgcggcgggg gtgcgggtac ggatcttctg
     2041 caacgccgag ggcgacgccg tcggttccca gggaggcaag gtccggcccg acgcgcccct
     2101 cgcggagctc cggcccgcgg agttcgatct ggtgtgcgtg atcgggggca cggggaccct
     2161 gaattacctc taccacgacg aagccctcgg ggagaagctc cgggaggccc gggatcaggg
     2221 caagcgggtg gccgggatct gcgcgggagc caccaccctg gccaagttcg gggtcctttc
     2281 cgggcgaacc gcgacgggag ccaaccgggg ggtcttcgtc cgggccggcg tcgcctactc
     2341 cttcgagaag gtcgtcgtgg acgggccctt catcacggcc cagggcgact accacgatct
     2401 cttcgccgcg gccctggtgc gggaacttac cggcggatga accgcggggt gcagatccgt
     2461 cggccgtgcc gccgtattga cagccgcccc cggcttcggg tataaaaccc gggtaccgat
     2521 tcacgtccgc ccagatggtg aaattggcaa acacgctaga ttcaggttct agtgccctaa
     2581 ccggcttggg ggttcaagtc cccctctggg caaaaaaacc gccttcaggc ggtttttttt
     2641 tgcccggagg gggacttgta tcgcccgccg cctcgggcgg cgccctacaa tccatacctg
     2701 gcgagaccgg caacgcagga gccggcaacg agggctcgtc gcgagccggc gagcgcgagg
     2761 cgcaggatgc cgtcgtgcgc gatagcgcgc atgtcaattt gcttcctctc ggcaaacgga
     2821 gcgaagcgac gtatgcgccg agagagcccg aggcgggccg gagcgacgag gcaggacggc
     2881 gaggagcgga ggccaagtcc ccctctgggc aaaaagagct cccttttggg gggctttttt
     2941 atgccgggac cggacgcctc acttgtggcg gtaggttaat tcttagtatg gtagataaat
     3001 aggtgatccg gccggtcccg gtacaggttg aaggcgatag ccccagcgaa tccagtgccg
     3061 ctccgccgcc ccggacaagg ctcaccgaat ttcacccgcg gatctcctcg atggcgttcg
     3121 cgagggactc cgaggtgacc ggcttgttca acaacaggct cggcccgagc tcgtcgatct
     3181 ccggaccgta atcctcccgg gtaaagcccg tcatgaagat gatcggcacg gggtgacgat
     3241 tccggatccg ccgggcggcc tcgatacccg tcatcgtacc ggccaggcgg atgtccatga
     3301 cgatgaggtc cgccggttcc tggtccatgc gttcgacggc cttctcgccg gtcgccaaga
     3361 ctgcgttgac cgcgaacccg gctcgctcca ggccgatctt caggttcaag gcgatgagtg
     3421 cctcgtcctc caccagtagc agcgagagcg gcttgcgcac ggcatcacac ccgcgccgcg
     3481 tacagctgat cgctgaagcg gattcgccag gtaacgccgt tatcgatttc aaagccggcg
     3541 tgaccttgaa gctggtgctc ggccaggccg atgatggtct ggaaacccag ggagttctgc
     3601 ttgcgatact cgaattccgg tgccactccc actccgtcgt cggagaagac gagctccagg
     3661 gtgagcgggt ccacccggga aaggcggatc cgaaccgatc cggggcgccc ctccgggaag
     3721 gcatgcttga agcagttgga cacgagttca ttcaggatca gaccgcaggg gatggcggtg
     3781 tcgatgaccg cctcgatatc ctcgatctcc gtttccagtc gtatgtcgcg ggtctcggtc
     3841 gaatacccgt ggagcaggag cgagagcaat tcgttgatgt actgccgcag gctcacgtgg
     3901 gacaggtcct tggattgata gagcatctga tgcaccaggg acatggcgcg gatacgccgc
     3961 tccgtatccc tggccatcgc ctcgaccgcc ggaaccccgg agtaggccgc ctgcagttcg
     4021 agcatcgacc cgatcacgtt catgttgttc ttcgtcctgt ggtacagctc gcgaaggagg
     4081 gtttctttct cggccaggga tttcttgagc cgggcgtcca ctcgcttgcg ctccgtgatg
     4141 tcccgccaaa tgcaatgaaa gatggaggct tcaccggatc ggatgacctg cgcgatcacc
     4201 agcacgtcca ggatctcgcc ctgcttcgtc cgctgcttcg tctcgaactc gtcatggccc
     4261 gtctcgatga tttttttgat gtgccggagg ctctcctcca gcgactcgat aacctcgata
     4321 tccgccaagg acaagcgggc gaactcttcg cgcccgtagc cgagctgccg gcagacctgc
     4381 tcgttgaatt ccagggggcg ggccgtggcg ggatcgagta tcacgacgcc gtcggggctt
     4441 tgctcgaaca gtgtacggta ccgatcctcg ctctcgcgcg aggcggcctc ggcggccttg
     4501 cgcgccgtgt tgtcgtggtg gaatccgaag aacaggttct ggtcccggat ataggtcacg
     4561 ctgatgtcca cgtcgaggag ggagccgtct ttcctccgga agcgggattc gaagagggcc
     4621 gacccttcgc tcgcgatctt ctccatccgg cgggcgactt catccgaatc gtcgatggcg
     4681 tcgatgtccg cgaccgagag ggcgagcaga tcctcccggg tgtagccgag catcgcgcac
     4741 atggaatcgt tcacgtccag gatccgcccg gaatcggccg ccaccatcca gaatccgtcg
     4801 agcatcttct ggatgacgac atcggcccgg gcctcgctgg cctcgatctt cttccgcgcc
     4861 tcgatttcgc gcagcttctt gaggcccgac tccgtgatga tctcgatgag tcccttgatg
     4921 aaatagaggt agtgctcgag ctggtcccgg gtccagatcg gtactttccg tatggcctcc
     4981 aggtaggccg cctcgtcgaa gccgtaccgg cgggcctgcc gggcgaagaa atccaggtcc
     5041 ggcggctcca ggaagaattg gccggtgaag aagttgccgt agtggattcc ctcgatgatg
     5101 atgggggtcg cgttgtcgac gagcccgtga gggcagcgat agctgaccgc cggattcgct
     5161 tcggagagat gatccaggat atatcggtcg cttttaaggc attccacctc gcactcgggg
     5221 ttcaggcgat ggaatttggt gcacgcgtcc tgccaggccg tcgcggtcag gatgacgccg
     5281 tcgttgtcga tgatggccga cgggaaggag tatatggcgt tgagccgatc ctggaggtgc
     5341 tggaactgct cgatgtcgat caggtccgcg agcttgtacg ccatgcctga ctccggatcc
     5401 ccgagtggcc tggaatccct actcctttta taaccattct agcgcttccg gtcgtcccgt
     5461 caaggcgttc cgcatccctt tcccggattt taacgaccac ctccggagcg tcgtctccgt
     5521 ccgggatccg gccggattcc gtccatcggc aggtcggata cctgaagggc ctccgcccct
     5581 cgcatccgtc cccggtctgc actacactta ctcccggagg caccccgtgg gcgacaaatc
     5641 cgcgatgagg aagaaggact acctggagga actggaccgc ctgaccgtgg agctcgttaa
     5701 gctccaggag tgggtcaagg cccgaaagct caaggtggtg gtactgttcg agggccggga
     5761 cgccgccggg aagggcgggg tcatcaaggc catcacgggg ggcctcaacc cccgctacgc
     5821 ccggatcgcg gccctggccg ccccgacgga gcgggagcgg aaccagtggt acttccagcg
     5881 ctacgcggcc cagctcccga ccggcgggga gatggtcttc ttcgaccgct cctggtacaa
     5941 ccgggccggg gtcgagcggg tcatgggctt ctgcaccgag gaggagtacc gggagttcgc
     6001 ccggtcggtg cccgaattcg agcgcatgct ccagcgggcg gggatcatcc tgatcaaata
     6061 ctggttcagc gtcagcgacg aggtccagga ggcccggttc cgggaccggc tcgaggaccc
     6121 cgccaaacgc tggaagctga gccccatgga catggacgcc cgctccaagt ggatcgagta
     6181 ctccagggcc aaggacgata tgttcgggtt ctcggacctg ccggactcgc cctggtacgt
     6241 tgtggacgcg gacgacaagt actgcgcccg gctcaacacc atcgtccact tcctggggaa
     6301 gataccctac ggggacctga ctcccgagcc catcgaactg ccgccccgcc aggagggcgg
     6361 cgactaccgg cggcctccca tcgagggcct gaagtggatc ccccgggtct accccgaacc
     6421 gaagggcaag ggcggcaagg cggcgaaggg cggcaagccc ccgaagccgg gcaaggccga
     6481 aaaggccgac aagaaagtca aaggcggtaa aaaggataag cccggcaaag ccgcgaaggg
     6541 cgcggctaag gaagacaccg gcaagtccgc caagccggac aaggacggcg gggagtaaat
     6601 cggccgcgcg aggtagccgc gggtcctccg ggaaggccga ccgaatcgtc gcggaacaac
     6661 ccggtaggcc cgagccgtat tcaggaagcc cgggagtata cctcgttcga gtggtcccgc
     6721 cgaattttcc gggatatttc tgagaaccag gatcggctcg ggtttcgtgc cgtccagcgc
     6781 ctagaccgtg cttggtctag atccggagat ccgatcccag ccgcgtttcc gtgtccgtcc
     6841 cggggttccg cgacctggtc cgggcgggta aggcccgcgg gcgccgccgc gcagtcgatg
     6901 ccgcagggag agaagaaggg ttccataaga gcctcctcgg aggtggcgcg ccggtccggt
     6961 cctccggatc gtccgccgtc ctgttgggaa ggctatcggg aaaccctgtc gaaacatgtc
     7021 aaggtttatc cgaattcccc ggtgtttcgt acaattccgc gaccctccgc gcccgggccg
     7081 cgaactccgc ccggagttcc gggggcccca ggacctcgat accgtccccg aagcccagca
     7141 ggaagtcgaa cagccactcc ccggccggca tgcggagttc cgcgatccgc cctccccggc
     7201 cgtcgggtgt cccgaaaccc cggcccaggt cctcctcgaa ccggggcagg gcggccggcg
     7261 cgacccggat ccggagatcc gtccgggtcc ctcccccgcc ccgccacgct tcctcggaag
     7321 gttccgggcg gggctcgaac ctctcgatcc tggttctcgg cctttcgatc cgggcgagct
     7381 tgaagtgccg ccaccccctc cggagccggc aatacgccca gaggtaccag ccctcccaga
     7441 ggtagaccac cgccgcgggc tccacggtcc gatcggtccg gcgcccttcc gcgtcccggt
     7501 acccgaactg gaccgtgcgg cggccctcga tggcgtcccg gaggagttcc accagggccc
     7561 gctcgtcccg gccgggagcc agggagatcc ggagccagga cgagcgccgg ctccggggcc
     7621 ttcccaggag ggcgtcgatc ttggcggccg cccgttccag cccccggccg ccgacggcct
     7681 cctcgacgcc cgccagggcc gcggaggcgg cggccagctc cgaccggtcc aggaccgatc
     7741 ggtccagggc gtagcccggc aggatccgga agcctccctc ccggccgggg atcgactcga
     7801 tggggcagcc ccaggcggac aggagatcga ggtcccggta cagggtccgc gcggataccc
     7861 cgaagcgccg ggccaggtcg ggggccgagt gcagccggcc tcccgccagg aggaggaaga
     7921 gcgagaggat ccgttccgcc cggaggctgg gggcgtccgg ggccgattcg tcgcgggtcc
     7981 gggtcatggg ccattctaac ccggctttgg aggccggtga agcgcgggat cccgccacaa
     8041 tcttcggaac accaggtcgg gttgcgtccc gggctccggg cgttatactg gaagcatgag
     8101 caagaacatt tccttcttct tcgactacgc ggacggggag ttcgacggcg agtccttcaa
     8161 cggaccctcc ctcatggcca ccctggactg cctcaccgcg caggcggcgg ccggcacgga
     8221 cacctgggag ggctacagcg cctggagcgt ggcgatccat gtcgcctact acaaatacta
     8281 cttcgtgcgg gcgatccggg gtcaggacaa ggcgggcgtc ttcccctatg ccaaggacca
     8341 gcacggcttc ggggatcccc cggaggtaac cgcggaggcc tgggtcgaga tgcgggcgta
     8401 cctccggaag atccaccggg cggtcatgga cgccctggtg gagctgggac cggagcgctt
     8461 cgcggagacc atgccccgct gggacatgcc ctacggcaag gcggtcgcgt ggctcctgaa
     8521 ccacgacgcg taccacgcgg cccagatccg caacatgggc gtcccgggcc tccgggagaa
     8581 acgaccgacc tgagcaggcg gatcccaggt cagcggtagc gcccggcgaa ctcctccagc
     8641 agggagtgca ggcgctcgtg ctccccggga cccatgacgg tctcctcgaa gcggtcgaag
     8701 tccccgccca gggcctcgag cctggaccga cccagcgcct ccgcaccggc ggggaacagg
     8761 atgccgtcct ccttctcgat gtgggcccgg aggagagcgg aatagccgga ggcggcgcgt
     8821 gcgaaggccg cgtagtcccc ggatccgagg gcgccgccca tgtcccggat gaaggacctt
     8881 ccgagccggt gctcctcgag catgactccg aggggacccc cttcccgggg catgccggcc
     8941 gcctccaggg cggggaagag gatgccctcc tccttgccgt ggtgacaggt gtccgcgaag
     9001 cccgagagga accggaccaa ctcgggaagg tccgcccggg ccgcggcatc ccccgtcgac
     9061 gcccgggcgg ccgcgcggtc caggattccg aggccgaaca ggatggcctc gtgttcgtgc
     9121 ttgaggatcg aaatggcgtc catacgtacc tcctacatcc aggtcagccc gcccccctcg
     9181 atgcccacga ccaccgtgga gatcgggacg gcgcgggacg ccgaggcggc cgcctcctgc
     9241 acgagccggg agaggcctcc gcagcagggc acctccatga tcgccaccgt cacggaggcg
     9301 acctcggatt cgtcgatcag ggcccggatc ttgtccaggt agacctcccg gccgtggtcc
     9361 agcttggggc aggcgatcac gaggcggcgc ccggccagca gggcctggtg gaaggccccg
     9421 caggcgaagg cggtgcagtc cgcggcgatc aggaggtccg cgccccggaa gtagggggcc
     9481 cggggattca ccaggtggag ctgtacgggc cactgctcca ggcgggaggc gaggcttccg
     9541 gaagcacccg ccccggacgg cccgagggcg ggcttcgggc gcgcgggggt cgaagccggc
     9601 gtacccgctc cggacgcgaa ggaccgggcc gcggagccgg gacagccccc ggatatgccg
     9661 ttgtcgatcc tcggggccgc gaaggaggcg ggagcggcgg gccggaggag ggaactcccc
     9721 cgggtacccg cggcggcgcg gggttcgggg aaggacgccc cgcagccggg gagggcgggc
     9781 tccggctctg ggacggccag gcccttttcc gcgagcacgg cccgggcctg ggccagccag
     9841 gtgtcctggc cgtggacgcg caggtggtcc aggtgggcgg ccacggtgtt catccccttg
     9901 ggcaggatgt tctcgatgac ccggcgctcg tcgtagtcct cgacctcccg ttccacggtc
     9961 cgtagggcgc cccggggaca ctccccgacg caggccccca ggccgtcgca gaggctctcc
    10021 cccaccaggc gcgcctttcc gtcgatgatg cgcagggcgc cctcgtggca gcccccggcg
    10081 cagaggccgc agccgtcgca cttctcctcg tcgatctcga tcaccgcacg tttcgccacg
    10141 ctcgtctcct tgtataaggt aatctggaac taaaatacct caataaaagc ccgagattcc
    10201 ccctgtcgag gtctcggcag tcgtcctatc cgcgccggac ccggtccggg cgcgccggct
    10261 ccggattcgc cccggcgatc cgggaaccgg ccgcggcccg gcggcctttc ggggaaccgt
    10321 cgggcttttt ccggccgccg tccccgggcc cggacaggaa ccgggggacc ccgtcccgta
    10381 cgatatccgc gatggtgcgc cgccccaggt aggccacgag gccctcggcc tgccgggaga
    10441 gctcgtgccc gaagatgcag gccgaaggat cgcagtggtc cgggctgaag gggcagaagt
    10501 ccccggtcag gggcccgtcg accgcctcta tcgcctcccg gaaggtgacg gccccgggat
    10561 cccgggcgag ccggaacccc ccgtggggtc ccttcacgga taggacaagc tcggcccgga
    10621 ccaggcgctg catcaccttg gccaggtggt tctcgctggc ccccatggac gcggccaggt
    10681 ccttgcccag gacgcggcgg tccggctccg aggccagggc cgccagggcg tgcaccgcga
    10741 tcagggaggc gtccgaaaga tggatgattc cgttcactgg cattatggta ttacaatacc
    10801 ataataaacc gaccgtgtca accgattctt gtcacggtgg aatgttcggt ggacctcaac
    10861 gttcgtccag gcgcaccagg gggcggagca ggccctcctg gccgttctcc ttgatcgagt
    10921 agagggccgc gagctgctcc ccgatctccc cctcgggcca gccggcccgg gcgaaccaga
    10981 ccacgtaggc ctccggaagg tcgatcagca aggttcccgc gtaccggccg aagggcatcc
    11041 gggcgttcgc caggcgaagg aggaagccgg gatcgtcgag catccagcta cagggtaccc
    11101 ttgtaggccc ctccgtccac ggcgatcatg gagcccgtga cgtaggaggc cgccggggac
    11161 agcaggaaga cggcgacgtt cgcgaactcc tcgggggtgg ccatgcggcc cagggggaag
    11221 tcccgggccc gctcggccag ctcggcctcc acggtcgttc ccttggccgc cgcgctcgag
    11281 gccacgagct tggtggcccg ctccgtggcc gtccagcccg gcaggatgga gttgaaccgg
    11341 atcccctgtc cgcccagctc cagggccagg gacttggtga gtcccaccgt ggccgcccgg
    11401 accgagttgg agaggatcag gttggggatg ggctgcttaa ccgagacgga ggtcaccgtg
    11461 agcacgctgg ccgacccgga acggcgcagg tgggggaggg cggcccggat gagacggacc
    11521 gcgctcagga agcagaggtc cacggccttg gcccaggccc cgtcgtcgaa ggactcgaag
    11581 gaccccgggg gcggaccgcc ggcgttcacc aggaggaggt cgataccccc cagggcttcc
    11641 gcggcacggg ccacgacggc ctcggggacg cccgcctccg acaggtcgcc gggaacccct
    11701 tccgcccgga tctcccgggc cgccttggcc agcttgtccg ggtcccggcc gttcagggcc
    11761 acccgggcgc cctccgcggc caggagccgg gcggtcgcga atcccaatcc gctggaggcc
    11821 cccgtcacca gggccctcaa ccccttcact ttcaagtcca tagcagctcc tcgtatggag
    11881 ccaattttaa aagtcggtca cttttgaaat tggctttgcg attgctcgcc aatcgcggat
    11941 ttcaaggtag ctttttcaaa acttcctgag ttttgaaaaa gccttttatg gttaaaaaga
    12001 tacagtggaa ccggagggaa gggaaggaag cggacctgta tatagggaag ggccgcggcc
    12061 ccgtcgacgg gaccgcggcc ctcgtaccgc ggaaaggttt acttcgcctt ctcgatggag
    12121 agcagctcga cctcgaagac cagggtggag aaggggggga tgaccccgcc ggcgccttcc
    12181 tccccgtagg ccagggagga ggggataaag agcttgtact tgctccccac cggcatcaac
    12241 tggagggcct ccacccagcc ggggatcact tcctccacca cgaactcggc gggctcgccg
    12301 cgctccacgg aggagtcgaa ggtcttgccg tccgtgaagg ttccggtgta gtggaccttt
    12361 accgagtccg tggccttggg cttctcgccg gagccctccg acaggaccac gtactgcaga
    12421 ccgctctcgg tggtcttgac cccgtccttc ttgccgttct cggccaggaa ggtcttctcc
    12481 ttctcggcgt tctcggcgtt cttcttttcc tgggcttcca tgaccgccgc ctggatgagc
    12541 tcctgggcct tttccatgtc gatggcgggc ttgtccttgt ccaggacgtc cttgatcccc
    12601 ttgaggaagg aggagtaatc cagggagacg taggcttcct tgagactgtt gccgatggcg
    12661 accccgaagg cgtagctcac gtccttcttg gaggccttgg gggcttcccc ctccgcgggc
    12721 ttgtcctggc ccttgcagga gaggaggagg gaagaggcga cgagaacggc aagaattagt
    12781 ttcttcataa cctatccaat aaagtgagat aggacgacgg gcgtccccgg ccgagctcgg
    12841 cctttcagct aagatagagg gaattcgaga aaacgacaag ccgtccggga tgtccgtggc
    12901 cgtcgcccgg ggttcggaga ggcccgcgtc acaaggttcg tgaccctagg gtcggcgggc
    12961 cttcccttcc ttgaggcggt ccgccagctt gagccccagg acggctgcgc cgagggcggc
    13021 caggacgcag gcccccaggc ccaggtacgt gaggacggtc cagcccaggg ccgagaggac
    13081 ggcccccagg aggagggatc cccgggcgcc ggacggaacc tcccgcaggg tgaagtggat
    13141 cgtgcagaag gagttgagct cgctgaagtc ccccagggag acccccagct cctggatctg
    13201 tccctcgatc tccaggatcc gggtctccag gtcgatccgg tccgccaggg aggccccgga
    13261 gtcccgcagg ccgaccaggc cgtcccgggt cttctccagg gacaagcggc gcgcctccag
    13321 ggcccggaag tccgaggtcc ggtccgtctt gatcacgttg gcggaccgga gatccccgat
    13381 agccttgagg gtctccacgg tcgggtcgaa ggcgtccggg atgactccca gggagatgga
    13441 caggacccgc gcgcccggaa ggccgtagga attctcccgc tggaccaggg ccttggcccc
    13501 ctcgatcgcc agcttgaccg cggccaggtc ggagtcccag tgcccggtcc tggactcgat
    13561 ctgggagacc cgctcgtact tctgctccac cacctgggct cccgaggggg cctgatccac
    13621 ggcgatcttg gcgctggcgt agttccggcg ctcctgcggg gggacggagg actggaccat
    13681 ctcgccggcc ccgaaccctc cgaagggcga cggcgccgtc cggtcccggg tcagctccca
    13741 ggccaggcgc gccgcgaaca ggaccgcgaa cagggccgcc gcggcgatca ggattctcct
    13801 cttccgttcc atggtgcctc ccttcggttc cgtcagccca gggccacgtc caagagcatc
    13861 atcagggcga agccccccag ggccccgagg gtggagatgt cggagtttcc ggatccctgg
    13921 gattccggta tgacctcctc caccaccacg tagatcatgg ctcccgccgc gaacgaaagg
    13981 gcgtagggca ggaggggctg ggccgcgacg accagggcgg cccccaggaa ccccgcgatc
    14041 ggctccacga cccccgaggc ttggccgtac cagaagctct tgaggcggct catgccctcc
    14101 cgacggaggg ggatggagac ggccgcccct tccgggaagt tctggagccc gatgcccagg
    14161 gccagggcgg cggctcccgc caggctcgct cccgggatcc ccgccgccag agcgccgaag
    14221 gccacgccca cggccaggcc ctccgggatg ttgtgcaggg tgatggcgaa cacgaggaga
    14281 atggagcgct gccaggaggt cttgggcccc tcggcgtcct ccagcttggc ctcgatgtgg
    14341 aggtggggca ggatcttgtc cacgagcttg aggaacaggc cccccgccag gaacccgatc
    14401 accgcgggga cccagggcac catgcccatg ctctcggcca tctcgatgcc cggggccagg
    14461 agggaccaga aggaggcggc ggtcatgacc ccgcccgcga acccgagcat ggcgtccagg
    14521 acccgccggt cgatcttctt gaagaagaag accatcccgg cccccagggc cgtcaccccc
    14581 caggtgaaga gggtcgccag gagcgcctgg accgtatgcg gaagccgggc gaaggtatcg
    14641 aacatggaag catcctttcc gggcgctccg gcggcgtttc cgcccgccgg tcccgcgcgt
    14701 tacaggtaga cggtgttcgg ccggagcagg tccgcggcca ggcaggaatg caccggacag
    14761 aggaaaacca ggtccccgat ccgcgtcgac gccaagtcct caggtcccat ccggacgatc
    14821 ccgtgctcct gggtcaccgt ccggaccccg gccccctccc ggaagcccgt ccaccggccg
    14881 tcccggacct cgaagacggc cccgtaggaa gtccccccct ccggaagggt ctgggactgc
    14941 ttggacaggt gcacggcccc cccgtagact acggcctccc ctcggtcggg gtagaccccg
    15001 acgaccgggc aggccacggc cgccgcgatg tccccggggg agcaggatcc cgcgacgtac
    15061 tgctggaggt cgtagtaggc gaagttcccg ggccggacct cgtccaggcc ggaccagtcc
    15121 tccacgaggc tcatagaggg ggtgtccccg aaggaggccc gcaggtccgg ccagccctcg
    15181 gaggccagga aggccttggc ggcaaggagg gcgtcccgac cctcccggaa gagggcggct
    15241 acggcctccc ggccccggac cccgtaggtc cgggaatcgt gggtcaggac ccccgccagg
    15301 cggaggcgcg gggccgctcg gaccgcctcg gacacagccc ggagtccctc ccggtcctcc
    15361 gcccggatcc cgcagcgacg ggaacccacg tcgatcttga tccacaggtc gacgggccgg
    15421 gagagccccg cggccagggc ccggatcgtc tccgggttgt ccgccaggag ccccaggcgg
    15481 atccgccccg ccagctcgtc catctccccg atctcccgga ggttggccgg aaaggcgatg
    15541 gtcaggtcgt cccagccgtc gtccgcgaag agcttggcca tctccacgga ggagaccgtg
    15601 ccggtccgga cccccaggtc ccggaagagg cgcccgatga cccgggactg gtgggtcttg
    15661 aggtggggcc ggaaggcggt cccgttccgg tccgcccgtc ccttcatcct gagcacgttc
    15721 tcccggacct tgccggtgtc caccacgagg gtggggcgcc ggatctcctc gaacatggaa
    15781 tacctccgtt ccggggggct ctcgctatcc ttcgagcatc ccgtcaaacg ctaacaatcc
    15841 agtccgccgg ccggtccgag atcccgggga ggcgcggggg aggagaagtc ccgggcggcg
    15901 gggtcggaga gggcggccgc cagctcgtcc atccgacgga gcttccggga caggtcccgt
    15961 gccaggttca tcatcaaaag ggcgaagatc ttggggtcca tgcagtagat ctgccggacc
    16021 gcccggttcg agatggccgc gacgcggagg ggcccgaggg cgcggaccgt ggcggccgag
    16081 ggctggacgt ccaggagctc catctccccg aaggtctccc cctccccgag ttcggtcagg
    16141 acgcgggttc ccttcacgat ttggacgcgg ccgtccagga tgaagtagac gctgtcgttg
    16201 gggtccccct cccggaggac ggactggccc tcggagtagg acgaggggag cagcagggga
    16261 cgtatccgct cgacctgatc cgggatcatc cccccgaaga gggagtactt ctggagtgag
    16321 gcggtgtcga tcacggacgc ctccgggcgg gcctacgccg gggccgcgga tcgggaaggt
    16381 tcataccctg acaaccatag cacgggcccc ggaggggcac aaggccgagg gaggggcttc
    16441 acaagcttcg taccaccggg agcgggttgc ccgatccgtc ccgggccgct aggatggcgg
    16501 acatgaagcg caaagccgga tttcccgagg acctcctgtt cggcgcggct cccgcgggaa
    16561 ccgcgcccgc cgagggcccg actgccgcgg cggccggccc ggcctccccg ggcgacccgt
    16621 ccccggagga ggccctcctc cgcgcgtacc tgcgggtcct ggccctcaag cgcctctacc
    16681 gacagggctg gctcaagcgg ggcgtcccgg aggaacggtg cgagtccgtg gcggaccaca
    16741 gcttcggcac cgccgtgctc gccctgttcc tggcgggacg ggaacccttc cgggacgcgg
    16801 accccggcag gacctgccgc atggccctgg cccacgagct gggggaggtc tacgccgggg
    16861 acatcacccc cgtggacggc atttcaaagg aagagaagta ccggatggaa cgggaatccc
    16921 tcctccgggt gctcgagggg gcgccgggat ccggggagat cctggccctc tgggaggagt
    16981 tcgaggaagg cgccagtccg gaggcccgcc tcctgcgccg cctggaccgc ctggagatgg
    17041 gcatccaggc cgcggtctac cgggcggagg gctttccccg gatggaggaa ttcctggaga
    17101 gcgcccgcaa ggccgcgggc gaggggctcc cgggggaggt gttggcgatg gcggtcaacc
    17161 gtccatcctc ctcagctgat cccgcaggtc ctcggtctcc ttgatccaga aggcccggcc
    17221 gccccagtcc gggaagtgcc ggaggaatcc cgcgtccccc cgctggcggg cctgccaggc
    17281 caggtaatgg accatccgca ggaagcgcag gggctcgatc agggcccgtt ccgcggaatc
    17341 gaagggccgg aactcctcgt acccctccag gatgagggcg aactcccgtc ggcattcgtc
    17401 cgaggggccg ggaaggagga gccacaggtc ctggacggcg ggccccatga ccatgtcgtc
    17461 gaagtccatg agcaacagcc cctctccggg ccggtccagg atattgcccc ggtggaagtc
    17521 gccgtggatg cgcaggagcc gggcctccgc caggagggga tccaggatct cccgggcccc
    17581 ctcgacggcc tcgcggaact cggcccggca ctcgggatgg ggcattccct cccgttccaa
    17641 ttcctcgaca taggccgaga ggagtccggg gcggaaggag atcctccgct ccgcggagcg
    17701 gagggccccc acggcgtgga cccggccggc caggcggccc aggcgcagcc agtcctcgtc
    17761 ggactccgca tcgaagagcc ggcccccccg acgggggaag agggcgaagg ggtagaggac
    17821 gtccccgccg tccggtgcct ccagggccag gaccgggagt gtatccccct ccgagtccgg
    17881 aaggggggcc gccaccggga tctccgccgc ggccagctcc gccaggaagg cgtgctcctc
    17941 ccgtatcgcc tccgctgtcc aacgtccggg gcggtagaac ttggcaacga agtcccggcc
    18001 gtcggcggac cgcagtccgt agacccggtt gacgtagctg ggatagggga cccagatccc
    18061 cttcaggtcc aggccgaagg cctcccgaac ggattccatc gcgagttcgg ggttcaggac
    18121 gtcgaaggga gcggtcatgg gtccactgta cggcacccgc cagccgcgcg gcaagcaccg
    18181 gctttaccga gccgggttcc ggcgcctata ctggccgccg tttgccggaa tttccgaggg
    18241 ggggagcgcc gcatgaggat tttcatcacg ggagccacgg gatatatcgg atccgcctta
    18301 gctctgtccc tgctggagga gggccacgag gtggccgccc tggcccggaa tccggaccgg
    18361 gggggcttcc tggcggcccg gggagcccgg gtcgtccggg gcgacctggg ggacgagggg
    18421 tccctggacc ggctctgcga gggggccgag gcggggttcc acctggccgg ctgggtcaag
    18481 gcctcggggt cccccgccga ctacgaggag gccaacgtgg ccggggcccg ccgggccttc
    18541 gaggccgccg tccgggcggg ggtccggcgg gtggtcttca cctcctccgc gggagtcctg
    18601 ggcccggccc tggacggatc ccccgtccac gaggactcgc cccggcgcat cggcttcttg
    18661 aacgactacg agcggaccaa ggcccaggcg gagatcctgg tcccgggctt cctggaccgg
    18721 gggctcgaga tcgtcacggt gaaccccacc cgggtctacg gctgtcccgt cggaccggac
    18781 ctgcccccct acacggccat gatcgaccgc tacctccggg ggaagttcct gctcattccc
    18841 ggggacggcc ggagcctggg caactacgcc ttcctggacg acgtggtcga ggggcaccgg
    18901 gcggccctgg cccggggcgt acccggacgc cgccacctcc tgggagggca cgacctgtcc
    18961 taccgggact tcttcgcggc cctggcccgg gcctccgggg tggagcgccg gttgccgaca
    19021 gcgccggcgg ccctcctcta cgccgcggcc ggcctggccg gggtagccgc ccggctgacc
    19081 gggggccgcc ccctcctcac ccccgcctgg gtcaagcgct tcctgtacga ctggaaggtc
    19141 tcctcggccc gggccgagtc ggaactggga taccaaatca cgcccctccc ggtcgccctg
    19201 gcccggacgg tccggggact gcgggaccgg ggcgggtact gagggcgcgg cttcgggcgc
    19261 ttcttcactc cgggaggcgc ccctggtata ctcgattccg aatccccgat ccctacccga
    19321 ggatagcccc atgctagacg cccgactgaa acgactggcc gagattctcg tgaatttctc
    19381 cgtggaggcc aagcccggcc agaaggtcct gatccaggcg accaacgcgg agacgccctt
    19441 cgtgaccgcc ctggtggacg ccgtccacga cgccggggcc cggcccttcg tcaccctccg
    19501 ggacaagacc gtggacaggg ccctgatccg gcgctgtacc gccgagcaga tggaggccca
    19561 ggcccggtac gagctggccc gcatgcggga gatggactgc ttcatcggct tcaccgccaa
    19621 ccgcaacctc tccgcctggt ccgacatccc cccggagaaa cttaagctct acaacgacat
    19681 cctcgcccgc aaggtccaca tggagtgccg ggtgccggac acccgctggg tggtgctgcg
    19741 ctacccgacc ccggggatgg cccagctggc ggggatgagc gaggagggct tcgaggactt
    19801 ctacttctcg gtctgcaccc tggactacgc caggatgtca aaggccatgg accccttggt
    19861 ggagctcctg gcccgcacgg accgggtgcg catcgtctcc cccggcacgg acctctcctt
    19921 ctcgatcaag ggcctgccgc cggtcaagtg cgacggcagg atgaacatcc ccgacgggga
    19981 ggtctacacc gccccggtca aggaatccgt gaacggggtc ctggcctaca atacccccag
    20041 cgagaaggac ggcttcacct tcgagaacgt gcggctggag ttcaaggacg ggaagatcgt
    20101 gaaggcgacg gccaacgaca cggacaggat caacgccatc ctggacacgg acccgggggc
    20161 ccggtacgtg ggcgagttcg ccctgggggt caacccgtac atcaccaagg ccatgaagga
    20221 gaccctcttc gacgagaaga tcgcggggtc catccacttc acgccgggca acagctacga
    20281 cgactgcttc aacgggaacc ggtccgcggt gcactgggac atggtcctga tccagacccc
    20341 cgagcacggg ggcggggaaa tctggatgga cgggcgcctg gtccgcaagg acggccgctt
    20401 cgtcgtgccc gagctggaat gcctgaaccc ggaaaacctg aagtaacgga tcccgcccca
    20461 tgaggccgag accccgcgcg cccctcctgc ccacggtcat cgtcttcgcc tgcgtcaccg
    20521 tggccgcggc cttcctggcc gcgggcatcc ccgtcgcctg ccagtcccgc ccggcgccct
    20581 cagcctcggg cgcgggcgcc acggcgccgg tcccggcgat cccctacagc atccacacgg
    20641 aacgcatccg ggagccctcg gccgcggatc cgtcccggat gacggtcgcc cgggtgctgc
    20701 gcgtggtgga cggggacacc gcggtcgtcg agatcccccg gccgcccgcg ggcctgggga
    20761 cccgggagac ggttcgcctc atcggggtgg acgccccgga gctcgggcgg gagagccggg
    20821 ccgcggatcc gggaggcccc gagtccgccg cggccgcccg ggcccttctg gaaggccgag
    20881 cggtcttgct ggccttcgac cgggagctcc gggaccggta cggccgggtc ctggcctacc
    20941 tgtattcgcc cgacggggtc tgcctgaacc tggagctggt gcgttccggg gcggcccggg
    21001 ccctcctgaa ataccccttc gccttctcct cggccttcgc cgcggcggag cgggaggccc
    21061 gctcggccgg ccggggactc cactccgaat tgtacattcc gtgaaaacta ggttagaata
    21121 tcgtttccca tcggaaggaa tccgatgagc agaacgggag gaatacctat gaaacgcatt
    21181 ctcgcagtcg cggcgatcct tcttgccgca ctttccttcg cggccgccca ggagaccctg
    21241 agcgtgtaca ccaccctgga ggaacccctg gccaaggcca tcctggagaa gttccaggcc
    21301 gagacgggga tccggaccag cttcgtccgc ctgtccggcg gcgaggccat cgcccggatg
    21361 gaggcggagg ccgccaatcc ccaggcctcc atctggctgg gcggggtggg cctggaccac
    21421 atcaccgcca aggacaaggg cctcaccacc gcctacaagt cccgcttcgc ctccaagacc
    21481 gccgcggagt tcaaggacaa ggacgggttc tggatcggcc tgtatgttgg ccccctcacc
    21541 ttcgtgacca acaccaagcg cgcagctgag ctcgggctca ccccgccgaa gagctgggcg
    21601 gacctattaa agcccgagta caagggccag atccgcatgg ccaacccgaa cacctccggc
    21661 acggcctaca atgtcatcac caccctccgc tacgtctaca agggcgacga ggagaaggtc
    21721 ttcgactaca tgaagaagct ggacgccaac atcgaccagt acacccgctc cgg
//
LOCUS       DAOTEI010000040        21310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1628_length_21310_cov_5.806822, whole genome shotgun sequence.
ACCESSION   DAOTEI010000040 DAOTEI010000000
VERSION     DAOTEI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 21310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21310
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_21310_cov_5.806822"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1235
                     /locus_tag="P5313_05810"
     CDS             426..1235
                     /locus_tag="P5313_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79913.1"
                     /translation="MRDTGGEAARDLPDSRASIAVRVSGESLFRRAWLAMGLRCFVPF
                     ALLLAAAGMLAARGVVDPFGRASAGWLWYLSAANAASLGFLAAAARREDLRMRDLYCA
                     RGSTWKGDLAWLAGFMVVSGVLSMVPGFLLAEAFWGGSSIPNNLLFGPLPEAAVYPLF
                     LLVPAGQGLAELPLYWGYAAPRLRASGWGRWRTILAVGAVLSLQHLFLSFRPDPLYAL
                     WLAFRFLPFALWTGWVIDRRPTVLPYLMGAHALLDAPLAVLALMVSRGAVL"
     gene            complement(1247..2320)
                     /locus_tag="P5313_05815"
     CDS             complement(1247..2320)
                     /locus_tag="P5313_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY79914.1"
                     /translation="MEPIRVGIIGFGMMGRMQALGCFGALADDFRLAAVCDDHTPHLA
                     AARELFGPDVRYFPDYRRMLDECDFDLAAVVTPDFLHEEMATACLRAGRHLRLEKPMA
                     LDPGGCARVIEAAEKAGTVVQIGLELRYAELVGRMRERREVLGRLKMLWCHEFRHPFL
                     AKPGSVPDWIVRREYSGGTLIEKCCHHFDLFNDFAGARPASVYAVGDHEVQYAHTDVL
                     DNAFATVEYENGTRANLSLCMFAPPAEGRPGLSTLDFGLLGDSGRMELRGDELSVWDR
                     GRPGVESYRHERVDLVGHDDEIVPSLRDLVRCIRTCGTPDADLRAGLSSVMTAWAAEE
                     SVRLGRPVEMREVEERFEVDYPA"
     gene            complement(2330..3166)
                     /locus_tag="P5313_05820"
     CDS             complement(2330..3166)
                     /locus_tag="P5313_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HRY79915.1"
                     /translation="MNWMEISPERLGEGSPIRVEIVENEFDLYWEIAFDMAERIRAAA
                     REDRPCLFIVPVGPVGQYRRLASLCNRERMDLRHVHLLNMDEYLDDRDRFIGLDDPLS
                     FRGFMDREFYGLLDPALAPPPENRVFPEPGREGEVARLVERLGGVDTSYGGVGINGHI
                     AFNEPPEPGEDPTVEEFAALPTRCLSLTRESRTINSVTAAGGCIDRIPRRCVTVGMKE
                     ILGARRLRFYMNREWQKGIVRKLLHGGITPRVPASLLALHPDAKLTITRTVAEPPLGR
                     LR"
     gene            complement(3163..4383)
                     /gene="nagA"
                     /locus_tag="P5313_05825"
     CDS             complement(3163..4383)
                     /gene="nagA"
                     /locus_tag="P5313_05825"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRY79916.1"
                     /translation="MTRTTIVNARILEGNDPGGPAGESGRGGAPGAADGPEGLRDLLL
                     GEGRILRISPAGRTPPEGVVIDAEGARAASGYVDIHTHGGAGADVMDADSEGLGRIAR
                     FHLSNGTTTFLATTLTAPLDRIRAALDTVRAYRGGGARIAGVHLEGPFLSPANAGAQE
                     KRFLRAPDTEALRFVADNADILRRVTLAPELPGALDLIAECAARGIGVSAGHDASVDD
                     EIEAAIETGLSCVTHIYCCSSTISRRTGPRKHLGLTELGMADPRLAVEVIADGRHVPD
                     ALFRLILRAKGRDSICLVSDSIRVAGMPDGEYRLGDPGTEVRVRKEGDEASVPDLGVY
                     AGSVMPLGAMVRRLVAGGAASLEDAVHMASAVPARLAGLSDRGRIAEGLLADINLLAE
                     DGSVLRTFLGGQAT"
     gene            complement(4504..5760)
                     /locus_tag="P5313_05830"
     CDS             complement(4504..5760)
                     /locus_tag="P5313_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79917.1"
                     /translation="MISRKSFRVTALALMILMVLPLAVSAQGKEIVLKWPSIWVGKDS
                     KAPAIAQIVESFNKANAGKIRVEVEPQPDYNAYEQKVRTSLAAKQVPGDIFTFNLNAT
                     TREFYDSPLLMDFSKHLTGEWKASFDAGAVAQSTVNKKLKSLPYETAILPIWYNTDLL
                     KKAGVTSIPGTLPEFWAAMDKLKASGVTPMSQMSGDINAWTSMLWFSHLVVSQGGPNV
                     WDKPFTDKVFVESAKILKRMFVEYSTPDAVGAGAGVSGGHFLAGRTAVFSNGPWYAGR
                     ADLRATPFFNSIVVAPAPVIGPNRNMMISILQSNIAAGETKDAARREAILAFLKHLTS
                     RESIALLQSTSGAMFAIDSGFIPLDPFQKQFYDLAAKSKITSHHLQAAYGAEATLEFA
                     QQLGALVLDKITPEQFCALVEKKIDR"
     gene            complement(6090..6287)
                     /locus_tag="P5313_05835"
     CDS             complement(6090..6287)
                     /locus_tag="P5313_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79918.1"
                     /translation="MDLLNTMDSAAFRNHLAAPVPALYRDMDDTARASSLNVTRKEAN
                     ERVSLGFPRETWVQSWAVAPF"
     gene            complement(6419..7456)
                     /locus_tag="P5313_05840"
     CDS             complement(6419..7456)
                     /locus_tag="P5313_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRY79919.1"
                     /translation="MPITPESAQIPAVEVRFSYTAELVLASMLVTGRPASITGFDEAW
                     QKAQTAALPPAALRFLEKTRGFSCPTLSFIDFVARTREFDDPERLFAEVRRLPVRRFL
                     SILLNEDLSDPDLDECLAEPRKAAYHVSKLSFFSRMPESDLVSIFTDPQAFREDLIGF
                     VAANRTEGFEARIRDLAPVYAGRIAAMQTRLAAESPLQVIQDIKKHPPYRSESLRYVF
                     LPSHFEGYMNISCHDEEQYMLLFTIVPPPPGDAAGIRIAEKLRALSDRSRMEVLRLLS
                     TAPSYGKEISSRLGLTTATVSRHLDQLKKAGLVIEEPADAQNVKIVRLDRAGVEQLLD
                     ETRRFLFGESS"
     gene            7899..8699
                     /locus_tag="P5313_05845"
     CDS             7899..8699
                     /locus_tag="P5313_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY79920.1"
                     /translation="MKDLDTHGVVFDTWRVESCGDHAIHLFTAALKAVHPRGGTVVVL
                     PSPCIRESLRISRLETLFPPARDPTEAAAILAAAAADRSPGAPAPDLPREEEDGNGNK
                     AVFEPLSDLPGGLVIRYSGYLDRQAGTWFYGLVLRRVEAEFIRLALDFSGLQWPGSGI
                     GHLTGILKTVKLRDGRLVLFGIPPKIDEVFLLLGLFHQYFEIVDTEAEAMALLKRSSD
                     GLEEPEFPKIFKCPICGARTRSARPMRGRCKSCKTLLKVTDEGEVFLG"
     gene            8692..9861
                     /locus_tag="P5313_05850"
     CDS             8692..9861
                     /locus_tag="P5313_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRY79921.1"
                     /translation="MAELPPLPRRRRRPLPLILDTDLNEDVDDAGALAVLHALADRGL
                     AEILGMTYCTSARWGVPAIRAVNRWYGRPDIPVGAFGRPGFLDEHADLSYIRFLAEAY
                     GPGEGVAAPGGPEPAAVSAQAEAVPGNNPEAAAAPAQAGTAHEADPEAVGLIRRLLAG
                     RDRADAVLVGIGPMLNLAALLDSGPDGTSPLSGPDLVRRSVRKLVLMCGRIPEGIEYN
                     LRQDPGAARRVLAGWPTPVVICDGELGGSLQAGARFCREAPRGNPVRTAYRLHTVGLD
                     WACYDQAAVLAAVTGTRYFRLSAPGRLEVDREGSGRWTADPSGNHRYLEARFDPADLS
                     RLVEDLMLAPPRSGAPAPFLPSASDEELLERRKAALAAHFRAVLDEGLEELEGAE"
     gene            9858..10628
                     /locus_tag="P5313_05855"
     CDS             9858..10628
                     /locus_tag="P5313_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY79922.1"
                     /translation="MRLLFSSDLHALMPAYRLFARTLAEGPWDAGVLAGDLLDDIRLP
                     DEFLVRELGADPDDLLEDLADPEDPSPWQTVGRRFNEINLRGLSILEGRFREVLGGAG
                     KPVFLVPGNHDDTPWADGGGIVNLHGRRIEFGTWNFVGYRWTVDHRSEADLDREVRAL
                     APRVDRKSILVTHSPPAGILDGRAELIHGIPALRDLVRDREPRLHLFGHIHNRPGRRG
                     RFVNGAYPHARVFWDIEVETRKVRRIPAAVPVPNPRWA"
     gene            10625..11224
                     /locus_tag="P5313_05860"
     CDS             10625..11224
                     /locus_tag="P5313_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79923.1"
                     /translation="MTLPILVFGEEELADLVRVGVPLPDHLVSIGNPRVPWAEREPGT
                     RVRPEFRPRFRSILRLSFQDATERSHLRHPFLARIPEVRDARRVVAFWRRTRREASGY
                     VVHCWAGVSRSTAAALALLYLETGSEAEAVRRLREVRPQALPNLRFVRCFDRILGSRL
                     EAEAKVMQDSRIADFARELASLMEELPVVQDAEPVEEES"
     gene            11221..12132
                     /locus_tag="P5313_05865"
     CDS             11221..12132
                     /locus_tag="P5313_05865"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRY79924.1"
                     /translation="MNDGRRILARLSLLGLSAGDAYGEACFSRDPVEVAARIEAGQVP
                     PGSLRWTDDTAMAVSVVETLEARGRIDPDDLARRFALRYAEDPARGYWGGAHAILSEI
                     ARGKDWRAVSRAAFGGTGSWGNGGAMRAAPIGAWYAGDPARAASEGATSAEPTHAHPE
                     GIDGAAAVAAAAALAAASGREGTGSFDRDAFFTAVLDVLGSGRVREGIGKARVLGDVP
                     AERAAWSLGSGMAVAAFDTVPFCLWVAARRPSDYAAALRDAACGIDRDTTCAIVGGIV
                     ALSAPGGVPAAWARAAEPLPESNKEAP"
     gene            12129..12575
                     /locus_tag="P5313_05870"
     CDS             12129..12575
                     /locus_tag="P5313_05870"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY79925.1"
                     /translation="MNVELRPVDKDNFEEIVAMPKQDHVAANIRSLAQAWLYPSLRPR
                     AIYADGKLAGFCMSGVDDDADDPADRGIPWIVRMYVLPDLHGRGIGSAAVRLLVAEIR
                     RDYPAAARVRLSTHPENAHAIKVYLKAGFRDTGILDDGEEVFELAL"
     gene            complement(12641..14188)
                     /locus_tag="P5313_05875"
     CDS             complement(12641..14188)
                     /locus_tag="P5313_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY79926.1"
                     /translation="MKRRPRDSEELEELEAVEEDESVEELESVEDAEPVPQDSEAVPL
                     YSELPDFASPEASLGPPGVPADESIRRGFEAEMRILQAELERIRQAKSDAARHETPTS
                     GRKHTKEKPMEELRDPGSSPQTYGTKEYIKRRRKLSGLFFETALPNEYLVEVGRKDVR
                     PRLGGRRFRLFRKFLRIPASVQTLVFTTDNANVDYQGIGVDGYASWRVDPEHPEVAVR
                     TLDFFDEDDPMARTNAELRTICVEAVRHVISNMSIDDALKKKDEIAENLSRQLKGIER
                     KWGILFDQVGIEKVRIMSDRLFEQLQAQFRDGLRLEVERKRIATDRQIAAEQNAMREG
                     TGLETILTNQKLELSEVEQKARVQEAQAAEAHRLALQKIEIDRATFREEQSFRTEKEK
                     EGHALALLERTLKTELAAAELRLLKEESSVEDLRAKIGERQLELQRLQREVSQVFSDQ
                     ALASEFIQRLPSLFAALQIDNYTVLDSGSGQVTPVTRVLTEVAAALKGLDLKGLLGGG
                     KDAAPGA"
     gene            complement(14229..15209)
                     /locus_tag="P5313_05880"
     CDS             complement(14229..15209)
                     /locus_tag="P5313_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY79927.1"
                     /translation="MKIHVIGCGNAFSSESFNQSFVIEEAGRRMLIDCGMQTPQALVT
                     ARYKPQDIHDIYVSHLHADHVGGLEYFAFARYDWAKHPRSWSEGTYAPRLIGNVQLLR
                     DLWDKTLRGGLESMEGFVSNLETFFEPVPVEPNKRFEWQGWTVDLIQQIHIMSGSVIM
                     PSFGILFSRTGRKSVYFVTDSQHCSPRQIEEYYKRADLIFQDCECTGMDLRFPEGAKT
                     YVQDGVPKAWPEDEMEALDLLARGIEPVKWSRYKFSSGVHANYAQLAGYESANSIKLD
                     PQVKAKMWLSHYQDYVLIGKDHFGNEVDWAAEAKRDGFAGFVKLGQRFEV"
     gene            complement(15303..16601)
                     /locus_tag="P5313_05885"
     CDS             complement(15303..16601)
                     /locus_tag="P5313_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY79928.1"
                     /translation="MDPLAISQALSALVFGALSAGSLLLGAWTGIFLKPPPRVSAAVM
                     AFGAGALFAALSLELIGEAVERLHGNFWPIGLGCVAGGLLFVTLDHLVNGAGGFLRKR
                     STLSRRLRADRRKDVRLLLESLAKVPLFASLPPEEVRSLADQVTRVRCDAGTPVIRTG
                     EPGAELYLVESGRLEVSRGGASLAVLGPGDMVGEMALLTGETRTADVTTLEAAVLLTL
                     RKEAFDRLLAANPRLREAVERLSGQRREALEKAAATVDPAAWARKAGTGDWKPSSLEL
                     DEAFAQAGGKKKGGAPMGIWLGILLDGVPESAVIGASMIGAEKASLALILGLFIANYP
                     EALSSAVGMRKMGMSVWKITALWGSIVALTAVGAGIGNLAFRSAPGELVALFESLAAG
                     SMLAMLAETMMPEAYEHGGSVTGMATILGFVSAVFLRVAG"
     gene            complement(16911..18974)
                     /locus_tag="P5313_05890"
     CDS             complement(16911..18974)
                     /locus_tag="P5313_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRY79929.1"
                     /translation="MLGAISGDVLGSLYEAWSIKDKNFRLFHELDRPTDDSVLSLAVA
                     KAILDADAEGRLPEAGDYERELRAFGRRYPDAGYGGSFRAWVNDPVGKPYESFGNGSA
                     MRASAVGWAFGDAETVLTQAELSAAPTHGHPEGIKGAQAVALAVLRARTGSSKEEIRS
                     ELSRRFGYDLGRTVDSIRPGYPFEVSCQASVPEAIVAFLDSRDWEDAVRNAVSLGGDA
                     DTQACIAGAIAEAFYGGVPPRVLAWVLPRLEAGLLSTLSDFARRFLPADRAAAVAAET
                     AARGLPRGVPGAGGNRKAGGAGPAPGDRKRRGAAHAGDPGRDPDTRHPLAPAPGYRVV
                     LAGSTRARIADYARRLSADPGLAGARLQAALADLAVPRFDAAWLLVSLLATKKPRIFA
                     ESEVAGDGSDWTREELGLLGDIGFAVPVTVFDDGRHSAPRVHSTPFGATLLFAPGALL
                     AGRRGSPADGHVAPGGHIDPVRYGALYEGRLLPLLQYASAVSSAAGRKALVTVPGLGC
                     GQFAGPFRGRLGPYLRDALAGILKRNAKILPGIRAVRLDPYDECGNEDRRIGRIAFRV
                     RPLAVNERKGTGLGLPQLRPPRDYQEPGDDFSDCDLFSVVAWDPVSWPGNDYWAGARA
                     TDDGVKAAATDAMFALSGFRGRYDPETCGYLPPPGVRAWEDLVLEQRLVLRAVDNVTV
                     VGSPG"
     gene            complement(18984..19670)
                     /locus_tag="P5313_05895"
     CDS             complement(18984..19670)
                     /locus_tag="P5313_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRY79930.1"
                     /translation="MYTYPYPHPAVTVDCVVFGLDEGELKVLLIQRDLDPFAGKWALP
                     GGFIRMDEDLEAAARRELEEETGVKKLYIEQLGAFGTPGRDPRERVVTVAWWAVVNLY
                     EHEVKASTDARNAAWFPVEELPALAFDHPVILDAALARVRETLRREPLAFEFLPRKFT
                     LTQLQRFYETVLGRPLDKRNFRKKVLSYGLLEELEEYEVDVAHRAAQLYTFRRDRYET
                     FREEGSRFLV"
     gene            complement(19716..>21310)
                     /locus_tag="P5313_05900"
     CDS             complement(19716..>21310)
                     /locus_tag="P5313_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRY79931.1"
                     /translation="GAAAAVGLLGPEDQASLVQFDDRVDLLQPLTPAGRAGALQAAIA
                     GIEPRGNTDLHGGWAAGADSLGDGEPGRGARRVVLLTDGLANHGVTDPGTIAAKVAEA
                     ARRGIATSALGIGEDFNEELLEAVARAGDGSYTFVESPDQLPAFFEAELAGFANLAGR
                     EVRLGLAPGPGIQIVSVLNDLPSSNTGSLVLPNLVAGGRQEIGLTVRALPGLTARPGE
                     ETELLAVRLEWTDPETGERREMRQAARVRAVEDRDFEALPADPAAVETLALLRTARAK
                     REALNAVDRGDEAGIALAMDHIRAILAGTPPTERTEREKISLASLEEDFRLRKMGKFR
                     KNVHYESYARGHAMDEARLFGHYSRKLLFLVRDRLDLHIGSLAGFPCDAIVNSRSDEP
                     ASGLDEALRAAGGPAFRAACEAIGRLGAGAAAVTGGGDLPVKSVIHVRVPDWRGGNAS
                     EFGALSECYAACLLVAKERGFGHVAFPVLGAGGRGFPVDAAVSLGLSAAAQFLFSNPA
                     PRRVTFVCPDGPAAELIWGQLP"
BASE COUNT         3125 a         7257 c         7521 g         3407 t
ORIGIN      
        1 ttgaaaacta agcaaatact tgctataatt ataaatagtg acaggcggtg ataatccaaa
       61 gtaatagaaa gttcgcctgt taaccactgg gtccggtgtt cgagtcaccg cgggggagct
      121 tgaaaaaacg acggcgtacc gtagggtacg ccgtcgtttt ttcaaggcaa cgaagcgctt
      181 cgcgcttcgt cgatccgccg gatatgcttg gcgccgaagg gcatcgccct tcggcgattt
      241 ccagtagttt gaaggcaacg aagcgcttcg cgcttcgtcg atccgccgga tatgctcgac
      301 gccgaagggc atcgcccttc ggcgatttcc agtagtttgt aaggtaacga ggcgccgcgt
      361 gcttcgtcga ccctcaggat gcactggaca gcgaacggcc gggccgaacc cgggaaggag
      421 gcgacatgcg ggataccggc ggagaggcgg cgagggatct tcccgattcc cgggcatcga
      481 tcgccgtccg cgtctcgggg gagtccttgt tccgccgggc ctggcttgcc atgggcctgc
      541 gttgcttcgt gcccttcgcc cttctcctgg ctgccgccgg gatgctcgcg gcccggggcg
      601 tcgtcgatcc cttcggacgg gcgtcggccg ggtggctctg gtacctgagc gcggccaacg
      661 cggcctccct cggattcctg gccgccgcgg cccgtcggga ggacctccgg atgcgggatc
      721 tgtattgcgc ccggggatcg acctggaagg gcgacttggc ctggctggcc ggtttcatgg
      781 tggtctccgg ggtgctctcg atggttccgg gcttcctgct cgccgaggcg ttctggggcg
      841 gctcctcgat cccgaacaac ctgctcttcg gtccccttcc ggaagccgcg gtctatcccc
      901 tgttcctgct cgtgcccgcg ggccagggcc tcgcggagct ccctctgtac tgggggtacg
      961 cggcgccccg gctgcgggcc tccggatggg gccgatggag gacgatcctg gccgtggggg
     1021 ccgtcctctc cctccagcac ctcttcctgt ccttccgtcc cgacccgctg tacgccttat
     1081 ggctcgcctt ccgattcctg cccttcgccc tctggacggg ctgggtgatc gaccggcggc
     1141 ccacggtcct cccgtacctg atgggagccc acgccctgct ggacgcgccc ctggccgtcc
     1201 tggccctgat ggtctcccgg ggcgccgtcc tgtagggggc tccctcctac gccgggtagt
     1261 ccacctcgaa gcgctcctcc acctcccgca tctccacggg gcggcccagg cggacggact
     1321 cttccgcggc ccaggcggtc atcacgctgg aaaggcccgc acggaggtcc gcgtccgggg
     1381 ttccgcacgt tcgaatgcac cggaccaagt cccgcaggga ggggacgatc tcgtcgtcgt
     1441 ggcccacgag gtcgacccgc tcgtgccggt acgactccac accggggcgc ccccggtccc
     1501 agacggacag ttcgtcgccc ctcagctcca tccgccccga gtcgcccagg aggccgaagt
     1561 ccagggtgga caggcccggc cgtccctccg cggggggggc gaacatgcac agggacaggt
     1621 tcgcccgcgt cccgttctcg tactccacgg tcgcgaaggc gttgtccagg acgtccgtgt
     1681 gggcgtactg gacctcgtgg tccccgacgg cgtacacgga agccgggcgg gccccggcga
     1741 agtcgttgaa gaggtcgaag tggtggcagc acttctctat gagggttccg ccggagtact
     1801 cccggcggac gatccagtcg ggcacgctcc cgggcttggc caggaagggg tggcggaact
     1861 cgtggcacca gagcattttg agccgcccca ggacctcccg ccgctcccgc atccggccca
     1921 ccagctccgc gtagcggagc tcgaggccta tctggacgac ggttcccgcc ttttcggccg
     1981 cctcgatcac ccgggcacag cctccggggt ccagggccat gggcttctcc aggcgcaggt
     2041 gtctccccgc ccgcaggcag gccgtcgcca tctcctcgtg caggaagtcc ggggtgacca
     2101 ccgcggccag gtcgaagtcg cactcgtcca gcatgcgccg gtaatccggg aagtaccgga
     2161 cgtccggacc gaagagctcc cgggcggcgg ccaggtgggg agtgtggtcg tcgcagaccg
     2221 cggccaggcg gaaatcgtcg gccagggcgc cgaagcagcc gagggcctgc atgcggccca
     2281 tcatcccgaa gccgatgatc ccgacgcgga tgggttccac ggccggttcc tagcgcaacc
     2341 ggcccagggg gggctcggcg acggtccggg tgatggtcag cttcgcgtcc gggtgcaggg
     2401 ccaggaggga ggcggggacc cggggcgtga tccccccgtg gaggagcttc cggacgatgc
     2461 ccttctgcca ctcccgattc atgtagaacc ggaggcggcg ggcccccagg atctccttca
     2521 tgcccacggt gacgcagcgc cgggggatgc ggtcgatgca gcctcccgcg gcggtgacgg
     2581 agttgatggt ccgggactcc cgggtaaggg agagacaccg cgtgggaagg gcggcgaact
     2641 cctcgaccgt gggatcctcg ccgggctcgg ggggctcgtt gaaggcgatg tggccgttga
     2701 tccccacgcc cccgtaggag gtgtccaccc cgcccaggcg ctcgacgagt cgcgcgacct
     2761 cgccctcccg gccgggctcc gggaagaccc ggttctccgg cggcggggcg agggccgggt
     2821 ccaataggcc gtagaactcc cggtccatga agccccggaa cgagaggggg tcgtccagcc
     2881 cgatgaagcg gtcccggtcg tccaggtact cgtccatgtt gaggaggtgg acgtgccgca
     2941 ggtccatgcg ctcccggttg cacagggagg ccaggcggcg gtactgcccc acgggcccca
     3001 cgggcacgat gaacaggcag ggccggtcct cccgggccgc ggcccgtatc cgctcggcca
     3061 tgtcgaaggc gatctcccag tagaggtcga actcgttctc cacgatctcc acccggatcg
     3121 ggctaccttc tcccaggcgc tccggggaga tttccatcca gttcatgtcg cttgtcctcc
     3181 caggaaggtc cggagcacgg acccgtcctc cgcgagcagg ttgatgtccg ccagcaggcc
     3241 ttcggcgatg cggccccggt cggaaaggcc ggccaaccgg gccggaaccg cggacgccat
     3301 gtggacggcg tcctccaggg aggcggcgcc ccccgcgacg agcctgcgta ccatggcgcc
     3361 caggggcatc acgctgcccg cgtagacccc caggtccggc acggaggctt cgtccccctc
     3421 cttgcggacc cggacctccg tgcccgggtc ccccaggcgg tactctccgt cgggcatccc
     3481 cgcgacccgg atgctgtcgg ataccaggca gatcgagtcc cggcccttgg cgcgcaggat
     3541 caggcggaag agggcgtccg ggacgtggcg gccgtccgct atgacctcca cggccaggcg
     3601 gggatccgcc atgccgagct ccgtgagccc caggtgcttc cgggggcccg tcctccggga
     3661 gatggtggag gaacagcagt agatgtgggt cacgcaggaa aggcccgtct cgatcgccgc
     3721 ctcgatttcg tcgtccacgg acgcgtcgtg ccccgcggac acgccgatgc cccgggcggc
     3781 gcattccgcg atcaagtcga gtgcgcccgg aagctccggg gcgagcgtga cgcgtcggag
     3841 gatgtccgcg ttgtccgcaa cgaaacgcag ggcttcggtg tccggcgccc ggaggaaccg
     3901 tttttcctgg gcccccgcgt tggccgggga gaggaaggga ccttccaggt ggacccccgc
     3961 gatccgggct cctccgcccc ggtaggcgcg gaccgtgtcc agggccgccc ggatccggtc
     4021 caggggtgcc gtgagggtcg tggccaggaa ggtcgtggtg ccgttggaga ggtggaagcg
     4081 ggcgatgcgc ccgaggccct cggagtccgc gtccatgacg tccgccccgg ccccgccgtg
     4141 ggtgtggatg tccacgtagc ccgaggccgc ccgggcgcct tccgcgtcga tgactacacc
     4201 ctccggtggc gtccggcccg ccggggagat gcggaggatc cggccctctc ccagaaggag
     4261 atcccggagg ccctcgggac cgtccgcggc cccgggcgct ccgcctcggc cggattcgcc
     4321 ggccggcccg ccggggtcgt ttccttcgag gattcgggcg ttgacgatcg tggtcctggt
     4381 catggagctt ggcctcggaa gactccgagc gcccctccgt tccggccggg gcgctcgggg
     4441 ttcaatgcgg atgaatcgat gttcccgaga ccgcggccgg agccgcgggt cgggattccg
     4501 ggatcagcgg tcgatcttct tctccaccag ggcgcagaac tgctcggggg tgatcttgtc
     4561 caggaccagg gcccccagct gctgggcgaa ctcgagggtg gcctcggcgc cgtaggcggc
     4621 ctggaggtgg tgggacgtga tcttcgactt ggcggccagg tcgtagaact gcttctggaa
     4681 gggatccagg gggatgaagc ccgagtcgat ggcgaacatg gccccggagg tggactgcag
     4741 gagggcgatg ctctcccggc tggtcaggtg cttgaggaag gcgaggatgg cctcccggcg
     4801 ggcggcgtcc ttggtctccc cggcggcgat gttggactgg aggatgctga tcatcatgtt
     4861 gcggttgggt ccgatcacgg gggcgggagc cacgacgatg ctgttgaaga agggggtggc
     4921 ccgtaggtcc gcccggcccg cgtaccaggg tccgttggag aagaccgcgg tgcggccggc
     4981 caggaaatgg ccgcccgaga cgcccgcgcc ggcgcccacg gcgtccgggg tcgagtactc
     5041 gacgaacatc cgcttgagga tcttggcgga ctccacgaag accttgtccg tgaagggctt
     5101 gtcccagacg ttgggacccc cctgggacac gaccaggtgg gagaaccaga gcatggaggt
     5161 ccaggcgttg atgtcgccgc tcatctggct catgggggtg accccggagg ctttgagctt
     5221 gtccatggcg gcccagaact cgggaagggt tccggggatg ctggtgacgc cggccttctt
     5281 gaggaggtcc gtgttgtacc agatgggcag gatggcggtc tcgtagggca gggacttgag
     5341 cttcttgttc accgtggact gggccacggc gcccgcgtcg aaggaggcct tccattcccc
     5401 ggtcaggtgc ttggagaagt ccatgagcag gggggagtcg tagaactccc gggtcgtggc
     5461 gttcaggttg aaggtgaaga tgtcacccgg cacctgcttg gccgccaggg aggtccggac
     5521 cttctgctcg taggcgttgt agtccggctg gggctcgacc tccacccgga tcttgcccgc
     5581 gttggccttg ttgaagctct ccacgatctg ggcgatcgcg ggggccttgg aatccttgcc
     5641 cacccaaatg gaaggccatt tgaggacgat ctccttgccc tgggcggaaa ccgccagggg
     5701 aaggaccata aggatcatga gcgcaagggc tgtgacacgg aaagactttc tggaaatcat
     5761 gccttcctcc tgcgggccgc gccgcttccg gcgtgcccgg cgtcgtcgtc tcgggtcctt
     5821 tcaaggttct tcggggcccg gtggaaatcc tctcgggttc cggcctctcg tacattcaag
     5881 agaacaacgg cggaacgcga agattacttt catcctgaaa gtaaggcacg gggaaggagc
     5941 tgtcaaggtt cgagatggca ggggggcgtg ggggattcgg tggagggggg gtcaggactc
     6001 gagcaaggcg aaacggcggc cccttccggc gaccgccgtt tccgcgagga atgctccgtt
     6061 tgagtgaggg ctcacgattc aggtcccgct cagaacggcg ccacggccca ggactgcacc
     6121 cacgtttccc gggggaagcc cagggaaacc cgctcgttcg cctccttgcg ggtgacgttc
     6181 aggctcgaag cccgggcggt gtcgtccatg tcccggtaga gggcagggac gggtgctgca
     6241 aggtggttcc ggaacgcggc cgaatccatc gtgttcaaaa ggtccataca tacctcctaa
     6301 agtgtgtagt tacctgatta cgtaattaca atacaaaaac agaaacacgt tgtcaagggg
     6361 ggaaatcgaa aattcgttca gatccgcggg atttcgtgcg cgggctggga gggtcgggtc
     6421 aggaggattc gccgaagagg aagcggcggg tctcgtccag gagctgctcg accccggcgc
     6481 ggtccagccg gacaatcttg acgttctggg cgtccgcggg ctcctcgatc acgagccccg
     6541 ccttcttgag ctggtccagg tgccgggata ccgtggcggt ggtcagtccg agccgggagg
     6601 agatctcctt gccgtagctg ggcgccgtgg acaggaggcg aagcacttcc atccggcttc
     6661 tgtccgagag ggcgcggagc ttttccgcta tccggattcc ggcggcgtcg ccggggggcg
     6721 gggggactat ggtgaagagg agcatgtact gttcctcgtc gtggcaggag atgttcatgt
     6781 atccctcgaa atgggagggg aggaacacgt accggaggga ttcgctgcgg tagggggggt
     6841 gcttcttgat gtcctggatc acctggagcg gggattccgc ggccaggcgc gtctgcatgg
     6901 ccgcgatgcg acccgcgtag accggcgcca ggtcccggat gcgcgcctcg aagccctcgg
     6961 tgcggttggc cgccacgaag ccgatcaggt cctcccggaa ggcctgggga tccgtgaaga
     7021 tggacacgag gtcgctttcc ggcatccggg agaagaagga gagcttggat acatggtagg
     7081 ctgcctttcg gggctccgcc aggcactcgt ccaggtccgg gtccgaaaga tcctcgttca
     7141 ggaggatcga caggaagcgc cggacgggga gtctgcgaac ctcggcgaag agccgctcgg
     7201 gatcgtcgaa ctcccgggtc cgggccacga agtcgatgaa cgagagggtg gggcaggaga
     7261 agccccgggt tttctccagg aaccggaggg cggccggcgg aagggcggcc gtctgggctt
     7321 tctgccaggc ctcgtcgaag cctgtgattg aggcggggcg tcccgttacg agcatggacg
     7381 ccaggacgag ttcggcggtg taggagaaac ggacttccac ggcgggaatc tgggcggatt
     7441 ccggtgtgat cggcatgcat ccataatagc gtaattgtcc aatatgacaa ctaccaaaat
     7501 gaaaaaccca aaaaccttta atgaaccggc gtcctgccag ctggcgaggg gaccacggcg
     7561 accatcgagc ttccgagggc ggggtaggcc gaagcgggga tccgggaacg cctccgcccc
     7621 gtgctatact cttcctggca gggtgctgac aaaggcgcca tcgcctttgt cagcaccctg
     7681 tgaatatgga acccaaggaa aacgatggaa aatcctggag ccccttcatg actccatccc
     7741 cggaacgacg gacggaccca accggcaagc tcggcctgac agcccaaccc ttgaaggacc
     7801 gagtgcccgg tacgccggag ggcaccctgc tcgtggaggt cgacggcaag tttgatcgag
     7861 ggaatgatgg ttgggccgtt gacctgcttc tgcggtatat gaaggatctg gacacgcacg
     7921 gtgtcgtgtt cgatacctgg agggtcgagt cctgcggcga tcacgcgatc catctgttta
     7981 cggctgctct gaaggcggtc catccccggg gcggaactgt ggtcgtcctg ccttcgccct
     8041 gcatccggga aagcctccgg atttccaggc tcgagacgct tttccctcca gcccgggatc
     8101 ctacggaagc cgccgcgatc ctagcggccg cggccgcgga ccgctcgccc ggcgctccgg
     8161 cgccggacct tccccgggaa gaggaggacg gcaacggtaa caaggccgtc ttcgaacctc
     8221 tgtccgacct ccccggcggc ctggtcattc ggtactcggg ctacctggac cggcaggccg
     8281 ggacttggtt ttacggcctg gtcctgcggc gcgtggaggc cgagttcatc cgtctggccc
     8341 tggacttctc cgggcttcaa tggccagggt cgggaatcgg gcacctgacc ggtatcctga
     8401 agacggtcaa gctccgggac ggccgcctgg tcctcttcgg aatccccccg aagatcgatg
     8461 aagtatttct gcttctggga ttattccacc agtacttcga gatcgtcgat accgaggcgg
     8521 aagccatggc gctcctgaaa aggagttcgg acggactgga ggagcctgag ttcccgaaga
     8581 tcttcaagtg ccccatctgc ggggcccgaa cccggtcggc ccggcccatg cgggggcgct
     8641 gcaagtcctg caagacgctc ctgaaggtga ccgacgaagg ggaggtgttc cttggctgag
     8701 cttcctcccc tcccgcgaag acggcgacgg ccgctacccc tgatcctgga cacggacttg
     8761 aacgaggacg tggacgacgc gggggccctg gcggtgctcc acgcccttgc ggatcgaggc
     8821 ctggcggaga tccttgggat gacctactgc acctccgcac gctggggcgt ccccgcaatc
     8881 cgggcggtca accgctggta cggccgaccg gacatacccg tcggcgcctt cggccggccg
     8941 ggcttcctgg acgagcacgc ggacctctcg tacatccgct tcctggcgga ggcctacgga
     9001 cccggggagg gcgtcgcggc cccgggcggt ccggaaccgg ccgccgtgtc cgcgcaggct
     9061 gaagccgtgc ccggtaacaa tcctgaggcg gccgccgcgc ccgcgcaggc cggaacagcg
     9121 cacgaagcgg acccggaggc ggtcggcctt atccggcggc tcctggcggg ccgcgatcgg
     9181 gcggacgcgg tactcgtggg catcggcccg atgctcaacc tggcggccct cctcgactcg
     9241 ggccccgacg gaaccagccc cctgtccggg ccggacctgg tccgccgttc ggtgcggaag
     9301 ctcgtcctca tgtgcggccg gatccccgag gggatcgagt acaatctccg gcaggatccc
     9361 ggggccgccc ggcgcgtcct agccggatgg ccgacccccg tcgtgatctg cgacggcgaa
     9421 ctcgggggct ccctccaggc gggcgcccgg ttctgccgcg aggcgccccg ggggaacccc
     9481 gtgcgcaccg cctaccgcct ccacacggtg ggcctggact gggcctgcta cgaccaggcc
     9541 gctgtcctgg ccgccgtgac ggggacccgg tatttccggc tctccgcgcc gggccggctc
     9601 gaggtggaca gggaaggctc cgggcgctgg acggcggatc cctccggaaa ccaccggtac
     9661 ctggaggccc ggttcgaccc cgcggacctc tcccggctgg tcgaggacct catgctcgcc
     9721 ccgccccggt ccggggcgcc cgcgcccttc ctcccctcgg cctcggacga ggaactcctg
     9781 gagcgccgga aggccgccct ggccgcccat ttccgcgcgg tcctggacga gggcctcgag
     9841 gaactggagg gggcggaatg aggctcctct tctcctcgga tctccacgcc ctcatgccgg
     9901 cgtaccgtct cttcgcccgg acattggcgg aaggtccctg ggacgcgggg gtcctggcgg
     9961 gggacctcct ggacgacatc cgcctgcccg acgagttcct ggtccgggaa ctgggggcgg
    10021 acccggacga ccttctggag gacctggcgg atcccgagga cccgtcgccc tggcaaaccg
    10081 taggccggcg cttcaacgag attaacctga gggggctgtc catcctggaa ggccggttcc
    10141 gggaggtcct gggcggggcc ggaaagcccg tcttcctggt gcccgggaac cacgacgaca
    10201 ccccctgggc ggatggagga gggatcgtga acctgcacgg acggaggatc gagttcggga
    10261 cctggaactt cgtcgggtac cggtggaccg tcgaccaccg gagcgaggcg gacctggacc
    10321 gggaggtccg ggccctggcg ccccgggtgg accggaaatc gatcctggtc acccattccc
    10381 cgcccgcggg gatcctggac ggccgggcgg agctgatcca cggcatcccc gccctccggg
    10441 acctggtccg ggaccgggag ccccggctac atctcttcgg ccacatccac aaccggcccg
    10501 gccggcgggg gcgcttcgtg aacggggcgt atccccacgc ccgggtcttc tgggacatcg
    10561 aggtcgaaac ccgaaaggtc cggcggatcc ccgcagccgt cccggttccg aacccgaggt
    10621 gggcatgacc ttgccgattc tcgtcttcgg ggaggaggag ctggcggacc tggtgagggt
    10681 cggcgtcccc ctgccggacc acctggtgtc catcgggaat ccccgcgtcc cctgggcgga
    10741 gcgggagccg ggcacccggg tgcgccccga gttccgaccg cgcttccgca gcatcctgcg
    10801 cctgtccttc caggacgcca ccgagcggtc ccatctccgg catcccttcc tggcccggat
    10861 ccccgaggtc cgggacgccc gcagggtcgt ggccttctgg cgccgcaccc gccgggaggc
    10921 gtccggctac gtggtgcact gctgggcggg ggtgtcccgg tccaccgcgg cggccctggc
    10981 gctcctctac ctggagaccg gcagcgaggc cgaggcggtc cggaggcttc gggaggtccg
    11041 gccccaggcc ctgcccaacc tccgcttcgt gcgctgcttc gaccggatcc tgggctcccg
    11101 cctggaggcc gaggccaaag tgatgcagga ctcgaggatc gcggacttcg cccgggagct
    11161 ggcgtccctg atggaggaac tgccggtggt ccaggacgcg gagcccgtgg aggaggaatc
    11221 gtgaacgacg gccgccgaat cctcgcccgc ctgtccctgc tggggctctc cgcgggggat
    11281 gcgtacgggg aagcctgttt ctcccgggac cccgtggagg tcgcggcccg gatcgaggcc
    11341 ggacaggttc cgccggggtc cctgcgctgg accgacgaca cggccatggc ggtctccgtc
    11401 gtggagaccc tggaggcccg gggacggatc gacccggacg acctggcccg gcgtttcgcc
    11461 ctccgctacg ccgaggaccc cgcccggggc tactggggag gggcccacgc gatcctgtcc
    11521 gagatagccc ggggaaagga ctggcgcgcg gtttcccggg cggccttcgg gggaaccggt
    11581 tcctggggca acgggggagc catgcgggcc gcgccgatcg gggcctggta cgcgggggac
    11641 cccgcccggg ccgcctcgga gggcgcgaca agcgcggagc ctacccatgc ccatcccgaa
    11701 ggcatcgacg gggcggccgc cgtagccgcg gccgccgcct tagcggccgc gtccggccgg
    11761 gaggggacgg ggtccttcga ccgggacgca ttctttaccg ccgtgctcga cgtgctcggg
    11821 tccggccggg tgcgggaggg gatcgggaag gcccgggtcc tgggggacgt gccggccgag
    11881 agggccgcct ggagcctcgg ctccggcatg gccgtggcgg ccttcgacac cgtccccttc
    11941 tgcctctggg tcgccgcccg tcggccctcc gactacgccg cggccctccg ggacgccgcc
    12001 tgcggcatcg accgggacac cacctgcgcc atcgtcgggg gcatcgtggc cctctcggcc
    12061 cccggcggcg tgcccgcggc ctgggcccgg gctgcggagc cgttgccgga atcgaacaag
    12121 gaggcaccat gaacgtggaa ctgcgccccg tggacaagga caatttcgag gagatcgtgg
    12181 ccatgcccaa gcaggaccac gtggccgcga atatccggag cttggctcag gcctggctct
    12241 acccgtccct tcggccccgg gcgatctacg cggacggcaa gctcgcggga ttctgcatga
    12301 gcggcgtcga cgacgacgcg gacgatccgg cggaccgggg catcccctgg atcgttcgga
    12361 tgtacgtcct gccggacctg cacggccggg ggatcgggag cgccgcggtc cggctcctgg
    12421 tggcggagat ccgccgggac taccccgcgg ccgcccgcgt gcggctgtcc acccacccgg
    12481 agaacgccca cgccatcaag gtctacctca aggcgggctt ccgggacacg ggtatcctgg
    12541 acgacgggga ggaggtcttc gaactcgccc tttgaggggc ccggcgcccg ggccccgccc
    12601 ctcggttcct tccgaacccg gcccgccgct ccctccccgg tcaggccccg ggggccgcgt
    12661 cctttccccc gcccaggagt cccttgaggt ccaggccctt cagggccgcg gccacctccg
    12721 tcagcacccg ggtgaccggc gtgacctggc ccgagcccga atccaggacg gtgtagttgt
    12781 cgatctggag ggccgcgaac agggagggca ggcgctggat gaattcgctg gccagggcct
    12841 ggtcggagaa gacctgggaa acctcccgct ggaggcgctg gagctccagc tggcgctccc
    12901 cgatcttcgc ccgcaggtcc tccaccgagg actcttcctt gaggagccgg agttccgcgg
    12961 cggccagttc cgtcttcagt gtccgttcca ggagtgccag ggcatggccc tccttctcct
    13021 tctcggtgcg gaaggactgc tcttcccgga aggtcgcgcg gtctatctcg atcttctgga
    13081 gggccaggcg gtgggcctcc gccgcctggg cctcctggac ccgggccttc tgctccacct
    13141 cggagagttc gagtttctgg ttggtcagga tggtctccag ccccgtgccc tcccgcatgg
    13201 cgttctgctc cgccgcgatc tggcggtccg tggcgatccg cttgcgctcc acctccaacc
    13261 ggagcccgtc ccggaactgg gcctggagct gctcgaacag gcggtccgac atgatgcgga
    13321 ccttctcgat ccccacctgg tcgaagagga tgccccactt ccgctcgatc cccttgagct
    13381 ggcgggagag gttctccgcg atctcgtcct tcttcttgag ggcgtcgtcg atgctcatgt
    13441 tggagatcac gtggcggacc gcctccacgc agatcgtccg gagctcggcg ttggtgcgag
    13501 ccatggggtc gtcctcgtcg aagaagtcca gggtgcggac cgcgacctcg ggatgctccg
    13561 ggtccacccg ccaggaggcg tagccgtcca caccgatccc ctggtagtcc acgttggcgt
    13621 tgtccgtggt gaagaccagg gtctggaccg aggcggggat gcgcaggaac ttacggaaga
    13681 gccggaatcg ccggcccccc aggcggggtc ggacgtcctt gcgccccacc tccaccaggt
    13741 actcgttggg cagggcggtc tcgaagaaaa ggcccgaaag cttgcggcgc cgcttgatgt
    13801 attctttggt gccgtaggtc tgtgggctcg agcccgggtc cctgagttct tccatgggtt
    13861 tctcctttgt gtgcttcctg ccgctggtcg gtgtttcatg ccgtgccgcg tcgctcttgg
    13921 cctgccggat cctctccagt tccgcctgga ggatccgcat ctccgcctcg aatccccgcc
    13981 ggatcgattc gtcggccggc acgcccggag ggcccaggga agcctcgggg gacgcgaagt
    14041 ccggaagttc ggagtacagc ggtaccgcct ccgagtcctg ggggaccggc tccgcgtcct
    14101 cgaccgactc gagctcttcg actgactcgt cttcctcgac agcctcgagt tcctcgagct
    14161 cctcggagtc ccgcggacgc cgcttcacgg ggacctcccg gacggttcca ttccggaccc
    14221 tccgtacctc agacctcgaa tcgctggccc agcttgacga agcccgcgaa cccgtctctc
    14281 ttcgcctccg cggcccagtc gacctcgttg ccgaagtggt ccttgccgat gaggacgtag
    14341 tcctggtagt gggagagcca catcttggcc ttgacctggg gatccagctt gatggagttg
    14401 gcgctctcgt agcccgccag ctgggcgtag ttggcgtgga cccccgagga gaacttgtac
    14461 cgggaccatt tcacgggctc gatcccccgg gccaggaggt ccagggcctc catctcgtcc
    14521 tcgggccagg ccttcgggac cccgtcctgg acgtaggtct tggcgccctc ggggaagcga
    14581 aggtccatgc ccgtgcactc gcagtcctgg aagatcaggt ccgcgcgctt gtagtactcc
    14641 tcgatctgcc ggggcgagca gtgctggctg tccgtcacga agtacacgga cttgcggccc
    14701 gtcctgctga agaggatgcc gaaggagggc atgatcaccg agcccgacat gatgtggatc
    14761 tgctggatca ggtccacggt ccagccctgc cactcgaagc gcttgttcgg ctcgacgggg
    14821 accggctcga agaaggtctc caggttcgag acgaaaccct ccatggattc caggccgccc
    14881 cggagggtct tgtcccagag gtcccggagg agctggacgt tgccgatcag ccggggggcg
    14941 taggtcccct ccgaccagga ccggggatgc ttggcccagt cgtaccgggc gaaagcgaag
    15001 tactccaggc ccccgacgtg gtccgcgtgg aggtggctca cgtagatgtc gtggatgtcc
    15061 tggggcttgt accgggccgt gaccagggcc tggggggtct gcatgccgca gtcgatgagc
    15121 atgcgccgcc cggcttcctc gatcacgaag gactggttga aggactcgct ggaaaaggcg
    15181 ttgccgcagc cgatgacgtg gatcttcatg aagggacgct cctttgtaat gattctctat
    15241 ccttcagtct atcacggttc cgggaatcca tcctgattat cgagggggct cggaattccg
    15301 aatcaccccg ccacccgcag gaagaccgcg gacacgaagc ccaggatggt ggccataccg
    15361 gtgacggaac cgccgtgctc gtaggcctcg ggcatcatgg tctccgccag catggccagc
    15421 atggagcccg cggccaggct ctcgaagagg gccaccagct ccccgggggc cgagcggaag
    15481 gccaggttgc cgatccccgc gcccaccgcg gtcagggcca cgatggagcc ccagagggcg
    15541 gtgatcttcc acaccgacat gcccatcttc cgcatcccca cggcgctgga cagggcctcg
    15601 gggtagttgg cgatgaagag tcccaggatc agggccaggc tggccttctc ggccccgatc
    15661 atgctggcac cgatgaccgc ggactcgggg acgccgtcca ggaggatgcc cagccagata
    15721 cccatggggg ccccgccctt cttctttccc ccagcctggg cgaaggcctc gtccagctcc
    15781 agggagctgg gcttccagtc cccggtcccg gccttccggg cccaggccgc agggtccacc
    15841 gtggcggccg ccttctccag ggcctcccgc cgctggccgg acagtctctc caccgcctcc
    15901 cggagccggg ggttcgcggc caggagtcgg tcgaaggctt ccttgcgcag ggtgagcagg
    15961 accgccgctt ccagggtcgt cacgtccgcg gtgcgggtct ccccggtgag cagggccatc
    16021 tccccgacca tgtccccggg cccgaggacc gccaggcttg cgcccccccg ggatacctcc
    16081 aggcggccgg actcgaccag gtagagctcg gccccgggct ccccggtgcg gatgaccggg
    16141 gtgccggcgt cgcagcgcac ccgggtcacc tggtccgcca ggctgcggac ctcctcgggg
    16201 ggcaggctgg cgaagagggg gaccttggcc agactctcca ggaggagccg gacgtccttc
    16261 ctccggtccg ctcggaggcg ccgggacagg gtggagcgct tgcggaggaa gcctcccgcg
    16321 ccgttcacca ggtggtccag ggtgacgaag agcaggcccc cggccacgca gcccagtccg
    16381 atgggccaga agttcccgtg gaggcgctcc accgcctcgc cgatcagttc cagggacagg
    16441 gccgcgaaga gggcccccgc gccgaaggcc atgaccgcgg cggagacccg gggcggcggc
    16501 ttcaggaata ttccggtcca ggcgcccagg aggagactcc ccgcggacag ggccccgaag
    16561 accagggccg agagggcctg ggatatcgcc aggggatcca tggaacctcc agtccggccg
    16621 cgccgaaggg agcgagtgtc cgggcggcgg ccgcctcggt tcaagtatcg ggcatggccg
    16681 ggccgggcgc cagagggagg gtgcgggctc acgaagcatg aggcggtcgg cttcgtcctc
    16741 ccgacgcgcg cgcaaacttt tttacatggt tttcaatggt gtttacatcg ttttccatct
    16801 tttttgattc ggcgccgatt ctcgtcacgt cacgatcgcc tccggagcca cgcggcttcc
    16861 aaggtccttc aggagttcgg gctcccggag gtccgggcgc ttcagccttc tcatcccgga
    16921 gatccgacga ccgtcacgtt gtccacggcc cgcaggacca ggcgctgctc caggaccagg
    16981 tcctcccagg cgcggacccc cgggggcggc agatatccgc aggtctccgg gtcgtagcgg
    17041 ccccggaagc ccgaaagggc gaacatcgcg tccgtggccg cggccttgac cccgtcgtcg
    17101 gtggcccggg ccccggccca gtagtcgttc ccgggccagg agacggggtc ccaggcgacg
    17161 acgctgaaga ggtcgcagtc cgagaagtcg tccccgggct cctggtagtc ccggggcggg
    17221 cggagctggg gcaggccgag ccccgtgccc ttccgttcgt tcaccgccag ggggcggacc
    17281 cggaaggcga tccgtccgat gcgccggtcc tcgttcccgc attcgtcgta gggatcgagg
    17341 cggacggccc ggatgcccgg caggatcttc gcgttccgtt tcaggatccc cgccagggcg
    17401 tccctcaggt acggtcccag gcggccccgg aaaggtccgg cgaactggcc gcagcccagg
    17461 cccggcacgg tgaccagggc cttccggccc gccgccgagg agacggcgct cgcgtactgg
    17521 aggaggggga ggagccgccc ctcgtagagg gccccgtacc ggaccgggtc gatgtggccg
    17581 ccgggggcta cgtgcccgtc cgcgggggat ccccgcctcc ctgccaacag ggccccgggg
    17641 gcgaagagca gggtggcccc gaagggcgtc gagtgtaccc ggggggcgga gtggcggccg
    17701 tcgtcgaaga ccgtgacggg caccgcgaag ccgatgtccc ccaaaagccc gagctcctcc
    17761 cgggtccagt ccgagccgtc cccggccacc tcgctctccg cgaagatccg gggcttcttg
    17821 gtggccagga gggagacgag gagccaggcc gcgtcaaacc tggggacagc caggtccgcc
    17881 agggccgcct ggagccgcgc ccccgcgaga cctgggtccg cggaaaggcg ccgtgcgtag
    17941 tcggcgatcc gggcccgggt cgagccggcc aggaccaccc ggtagcccgg ggcgggggcc
    18001 agggggtgcc gggtgtcggg gtccctgccc gggtctccgg catgcgccgc tccgcggcgc
    18061 ttccggtcgc ccggggcggg gccggctccg ccggccttcc ggttcccgcc cgcccccggt
    18121 actccccggg gcagaccgcg ggccgcggtc tcggcggcca cggccgcggc ccggtcggcg
    18181 ggcaggaagc ggcgcgcgaa gtccgagagg gtcgaaagga ggccggcctc cagccggggc
    18241 aggacccagg ccaggacccg gggcggaacc ccgccgtaga aggcctcggc gatcgccccg
    18301 gcgatgcagg cctgggtgtc cgcgtcgccc cccagggaga cggcgttccg caccgcgtcc
    18361 tcccaatccc gggaatccag gaaggcgacg atcgcctccg ggaccgaggc ctggcagctg
    18421 acctcgaagg ggtagcccgg ccggatcgag tccacggtcc ggcccaggtc gtagccgaag
    18481 cggcgggaga gctccgagcg gatttcctcc ttcgaggatc ccgtccgggc ccggaggacc
    18541 gccagggcga cggcctgggc tcctttgatg ccctccgggt ggccgtgggt cggggcggcc
    18601 gagagttccg cctgggtcag gacggtttcc gcgtccccga aggcccagcc cacggcggag
    18661 gcccgcatgg ccgagccgtt cccgaagctt tcgtagggct tgcctacggg atcgttcacc
    18721 caggcccgga aggagccccc gtagcccgcg tcggggtagc ggcggccgaa ggcccggagt
    18781 tcccgctcgt agtccccggc ttccggcaac cggccctcgg cgtcggcgtc caggatcgcc
    18841 ttggccaccg ccagggagag gacggagtcg tcggtgggcc ggtccagctc gtggaagagc
    18901 cggaagttct tgtccttgat cgaccaggcc tcgtacaacg agcccaggac gtcccctgaa
    18961 atcgcgccca gcatgaggcc tccttatacc aggaaccggc tgccttcctc ccggaacgtt
    19021 tcgtagcggt cccggcggaa ggtatagagc tgtgcggccc ggtgggccac gtccacctcg
    19081 tactcctcga gctcctcaag caggccgtag gaaaggacct tctttcggaa gttgcgcttg
    19141 tccaacggcc ggcccaggac ggtctcgtag aagcgctgga gctgggtcag ggtgaacttc
    19201 cggggcagga actcgaaggc caggggttcc cggcgcaggg tttcccgcac gcgggccagc
    19261 gccgcgtcca ggatgaccgg atggtcgaag gccagggcgg gaagctcctc cacggggaac
    19321 caggcggcgt tccgggcgtc cgtggaggcc ttcacctcgt gctcgtagag gttgaccacc
    19381 gcccaccagg ccacggtgac cacccgctcc cggggatccc ggccgggggt cccgaaggcc
    19441 ccgagctgct cgatgtacag cttcttgacc cccgtctcct cctccagttc ccgccgggcg
    19501 gcagcctcca ggtcctcgtc catgcggatg aagcccccgg gcaaggccca ttttccggcg
    19561 aaggggtcca ggtcccgctg gataaggagg accttgagtt ccccctcgtc caggccgaag
    19621 acgacgcagt ccacggtgac cgcggggtgg gggtacgggt aagtgtacat aggtctcgga
    19681 tcctccttcg acgggttaat aggcgggccc ggctttcagg gaagctggcc ccagatcagt
    19741 tcggctgccg gaccgtccgg acagacgaag gtcacccgcc gcggcgccgg gttcgagaac
    19801 aggaactggg ccgcggcact cagcccaagg gaaactgcgg catctacggg gaatcctctg
    19861 ccccccgcgc ccaggaccgg gaaggccacg tggccgaagc cccgctcctt cgcgacgagg
    19921 aggcaggccg cgtagcactc cgatagagct ccgaactcgg aggcattccc accccgccag
    19981 tccgggaccc gcacgtggat gaccgacttg acgggcaggt ctccgccgcc cgtgacggcc
    20041 gcggcacctg cgcccagcct gccgatcgcc tcgcaggcgg cccggaaggc ggggcccccc
    20101 gcggcccgga gagcctcgtc cagccccgag gcgggctcgt cggaccggga gttcacgatc
    20161 gcgtcgcagg ggaagccggc caggctgccg atgtgcaggt ccagcctgtc ccggaccagg
    20221 aacaggagct tccgggaata atgtccgaag agccgggcct cgtccatggc gtggccccgg
    20281 gcgtaggact cgtagtgcac gttcttgcgg aacttgccca ttttccggag ccggaagtcc
    20341 tcctccaggg aggccaggga gatcttctcc cgctccgtcc gttcggtggg aggcgttccc
    20401 gccaggatgg cccggatatg gtccatcgcg agggcgattc ccgcttcgtc cccccggtcc
    20461 acggcgttca gagcctcccg cttggcccgg gccgtccgga gcagggccag ggtctccact
    20521 gcggcgggat ccgcgggcag ggcctcgaaa tcccgatcct cgaccgcccg gacgcgggcg
    20581 gcctgccgca tttcccggcg ctccccggtc tccggatcgg tccattccag ccggaccgcc
    20641 aggagctccg tctcctcccc gggccgggcg gtcaggcccg gcagggcccg cacggtaagc
    20701 ccaatctcct gccggccccc tgcgaccagg ttgggcagga ccagggaacc cgtgttcgag
    20761 gacggaaggt cgttcaggac cgagacgatc tggatccccg gtcccggggc caggccgagc
    20821 cggacttccc ggcccgccag gttcgcgaat cccgcgagtt cggcttcgaa gaaggcgggc
    20881 agctggtccg gggattccac gaaggtgtag gagccgtccc cggcccgggc cacggcctcc
    20941 aggagttcct cgttgaagtc ctccccgatg cccagggcgc tggtggcgat gccccggcgg
    21001 gccgcctcgg ccaccttcgc ggcgatggtc ccggggtcgg tcaccccgtg gttggccagg
    21061 ccgtcggtca acaggacgac ccgccgggcg ccgcgtccgg gttctccgtc ccccaggctg
    21121 tccgctcccg cggcccagcc cccgtggaga tcggtgttgc cccggggctc gatgcccgcg
    21181 atggcggcct ggagggcacc ggcccggccc gccggggtca ggggctggag gaggtccacc
    21241 cggtcgtcga actgcaccag gctggcctgg tcttccggcc ccaggaggcc cacggcggcg
    21301 gccgccccct
//
LOCUS       DAOTEI010000041        21047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1655_length_21047_cov_4.430354, whole genome shotgun sequence.
ACCESSION   DAOTEI010000041 DAOTEI010000000
VERSION     DAOTEI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 21047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21047
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1655_length_21047_cov_4.430354"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="P5313_05905"
     CDS             <1..831
                     /locus_tag="P5313_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79932.1"
                     /translation="AGALAGFSERIRSASDSNESLKSLFEELKRDSESFLAAFKEISG
                     GTEEIAAGTAQMVDAVQELRVISTENRDAFGEIGRSVESLDRIFEEAAGFFEALGRDE
                     RTMSAAFGEAAGRVEALGLRGGESERSFSEIASELRYFSLDKGEENAAYRPEIKRIIF
                     DHKRRVVSGRLYLDGRVDARNLPARSRTEDCLLDPLLKRISPTLPERADMLGRLDRAH
                     RAFHEAYNAFQEECLRPGPDEAGRRARASALFEEAELRWKALFDYREELNLVLEKAGG
                     "
     gene            complement(1086..1619)
                     /locus_tag="P5313_05910"
     CDS             complement(1086..1619)
                     /locus_tag="P5313_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRY79933.1"
                     /translation="MTEKSFMAGFGGQGIISMGQLWVYCGMKEGLEVTMFPFYGAEKR
                     GGIARAGCVVSTTEIASPLVTTPDSAVVMNEDSLPLCEGIVKQGGTLLINSSLVKTET
                     KRKDCRVVKVPCNEIAEKIGDGKIANMVMMGALSKVTGAVKLDKLEPVLKSFFPPSKH
                     RFIEMNLKAIEAGKSAV"
     gene            complement(1616..2371)
                     /locus_tag="P5313_05915"
     CDS             complement(1616..2371)
                     /locus_tag="P5313_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY79934.1"
                     /translation="MELAYGHPKSLKPIQTKYCPGCGHGVIHRIICEVIDELGLQEKS
                     IITNPVGCSIWADLYFDIDSVQPAHGRTPAAATGVKRVRPDHLVICYQGDGDLAAIGT
                     AEIVHAANRGEKFTTIFVNNAIYGMTGGQMAPTTLVGQKATTAPYGRDPEGAGMGYPI
                     HVLEMLATLEGTRYLARGSVNNAANVRKTKQYIKKAFEAQMSGVGFTMVEILSQCPTN
                     WSMDPQESVEWLEKNMIPFYQLGEVKNTLEASK"
     gene            complement(2381..3448)
                     /gene="vorB"
                     /locus_tag="P5313_05920"
     CDS             complement(2381..3448)
                     /gene="vorB"
                     /locus_tag="P5313_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRY79935.1"
                     /translation="MAEEKRLMKGNDAIGEAAIRAGCTAYFGYPITPQNELTAYMARH
                     MIDHKRTFIQAESEVSAINMVFGAAAAGARAMTSSSSPGISLKQEGISYACGADVPCV
                     VVNVARSGPGLGGISPGQGDYFQATRGGGHGDYFHLVLAPKSVQEAAELTFEAFDLAD
                     QWRMPVMILADGMIGQMMEGVTFPEPRDLEKLPARPWAAGGQYKTHRPINHVSSINLV
                     PDELEAKVHERFLRYEQIKKKEIRFEEVNTDKADLILVAYGTSSRVCLGAQQMAKALG
                     IELGIFRPISLWPFPYDRIAALAAKGKRFLTVEMSMGQMVQDVQLAVAGKAQVSFYGR
                     CGGNIPTEEAVLAEARKLLGK"
     gene            complement(3462..3677)
                     /locus_tag="P5313_05925"
     CDS             complement(3462..3677)
                     /locus_tag="P5313_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY79936.1"
                     /translation="MAIKGKPEIDRERCKGCMLCVRACPTKVLEADAAPNSWGYYPAR
                     AAAPDKCIACGNCYLVCPDVAVTVYKL"
     gene            complement(3752..4663)
                     /locus_tag="P5313_05930"
     CDS             complement(3752..4663)
                     /locus_tag="P5313_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HRY79937.1"
                     /translation="MKTIQEILDRARSLGRKRIAVAAAQEHSALEAACAAAEHGVADP
                     ILIGDLAAVDRIAGELGLDVSRFQRIEEKDSGKAAARAVELVREGRADLLMKGILDTS
                     VLLKAVLNKENGLNTGRLTSHVAVMETQTYHKLLFVTDAAINIAPDIPGKIDIIANAV
                     QVAQALGIPVPKVALLAAVEKVNWEKMPCTADAAVLTQMNRRGQIKGCLVDGPLALDN
                     AVSAERARIKGIVSEVAGDADIFVAPDIEAGNILYKCLLDLGQAKGAGIVVGAARPVV
                     LTSRADTADVKLASIALACVVATGSEQ"
     gene            complement(4665..5729)
                     /gene="buk"
                     /locus_tag="P5313_05935"
     CDS             complement(4665..5729)
                     /gene="buk"
                     /locus_tag="P5313_05935"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRY79938.1"
                     /translation="MATILVINPGSTSTKIGVFRDLEEVFTETVNHSAEEIGRYASIA
                     DQYGFREQHIRDALRKRGFDAHGIDAVVGRGGLLRPIPGGVYRVSSPMLEDLRAAKYG
                     EHASNLGALIADALGREWGVPSFIADPVVVDELSDVARVAGHALFQRRSIFHALNQKA
                     VCRRYARESGRAYEDLTLIVAHMGGGVSVGLHRRGRVTDVNQALNGEGPFSPERSGTL
                     PAGDLAKLCFSGKHSEKEVLKMITGQGGMVSLLGTNDMREVEKAVQAGDPKADLYYRA
                     FIYNVGKFIGSMAAAAAGKVDAIILTGGIAHGKGIQEGIAGLCGWIAPVVVYPGEGEL
                     EALALAGSRALSGEAHIQEY"
     gene            complement(6004..7875)
                     /locus_tag="P5313_05940"
     CDS             complement(6004..7875)
                     /locus_tag="P5313_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79939.1"
                     /translation="MARDIDVLQLLRSYATKNKVSTIDYRSFAQMVQRQAAQANRPEP
                     IYRDLASNPDVVLVPKLFQYARDRNLSINTVGGQIESISLPEHFTDLLLAEYRRMEEN
                     PDIPFPDDESIHAELPAEWVQAVPFYRLVFPEGIRPVLILSPMAADKVLEHAVLKVRQ
                     YLRKGGNKDFVQNKLSYAFPGKEMQLKESLSTILIKPFDAVRDMRTSGNDFTFALWAY
                     LASYMKQDVFKKSDKNPEDWAYLQAIYLCEVYNNFYKGKAQRAVERETAFKTLDTLLR
                     KSPYNFTLDEVTNFRDSMGRPLLGKYTREELEEWIRERTTRAETGRLPEILMPVTSRG
                     QRWLVAKDRVLPLVVRLLGEARASIRTSLIAEWKSVLEVYASTPTMEDDAAYRRDLEE
                     RVAKGYPILFVLISERIFSLVYNELRETPDAVPELDRFFSKGDLVPIDELLDLRRKHM
                     VTDVRNLLPIWYTLPILSGLFAFLFRLTSRKRARKERLASIAEKASEPKTEKTGTSAK
                     FSDRRIEFAAAAAKVEQVLLPEGYKLDEYLSVLINRWNTMLNPQAKADLTEDVNSLVR
                     DYLRGLLRSLKPSNFTAERVKGLAATLADTPSLLKIRNHSALEEYIRLYMVRLLKTR"
     gene            8332..8769
                     /locus_tag="P5313_05945"
     CDS             8332..8769
                     /locus_tag="P5313_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY79940.1"
                     /translation="MIAKSDMPNINEKRPDGVKPDGSPYRVLIVDDSMFIAKQLGQIL
                     TSEGFDVVGTAGDGVAGIEKYKELYPNVDLVTMDITMPKMDGVTALEKILEFDKNAAV
                     VMVSALGKEDIVKKSLLLGAKSYIVKPLDRKKVLERILTILGR"
     gene            complement(8792..9154)
                     /locus_tag="P5313_05950"
     CDS             complement(8792..9154)
                     /locus_tag="P5313_05950"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRY79941.1"
                     /translation="MGIRGELFSSKINCRGRSYFFNVKENRTGDVFLNIVESKPTENA
                     EFDRRSIVVFKEDMREFLQAFEKALRYIDTSQAPKKPRRAARPAVRPDGDSAKEGPRR
                     KMVVRKKDSEKGGESVED"
     gene            complement(9231..11132)
                     /locus_tag="P5313_05955"
     CDS             complement(9231..11132)
                     /locus_tag="P5313_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HRY79942.1"
                     /translation="MALILHPSTGFPVGDPSLRPLDPSREPSDADLEAALGSLILSAS
                     GWRKVFAGDGEEESLTPAVRPADLCLAACMARVFAERLADAASPGTAPALALGIDSRP
                     TGPALADVMVRVFLASGIVPKYAFIIAAPEIMAWTRREGSGLSGFCYISASHNPPGHN
                     GVKFGFADGGVLPASEADALAGRFRTLARDQAARRSVLEAASKVDERAVARVFSACSA
                     EKRSAVSAYTLFARRTAADREDLDEQERVLDDLARACESRPLGIVAEMNGSARSLSID
                     ADYLSMLGARPRLVNARPRAFAHRIVPEGASLDLCRKELEAARAQDPAFVLGYVPDCD
                     GDRGNLVRWSDASGGAEALEAQEVFSLAVLSELALLRGAGGEGSGKVAVAVNDATSLR
                     IDEIARTLGAEVFRAETGEANVVNLARALRAEGWTVRILGEGSNGGNITHPAAVRDPL
                     NTVTALVKLLALRGTGGGPAPFRVWMEASGRGGEYSPDFTLDDVLSSLPAWTTTSVFE
                     ERALLRISAGDPAELKRRFQARFREDWEARRGEFARSFGFVSWEARASAGSGERDATG
                     DFGAAGRGGLRILFRDGKGLPAGFLWMRGSGTEPVLRIMADLRGRDPEGETRLLEWLK
                     EMVKDSGVR"
     gene            complement(11159..11371)
                     /gene="csrA"
                     /locus_tag="P5313_05960"
     CDS             complement(11159..11371)
                     /gene="csrA"
                     /locus_tag="P5313_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRY79943.1"
                     /translation="MLILSRRLGERIVIGEDVVISVVEVRGDQVKLGIDAPRNVKVYR
                     QEVFDSIQQENLRAAESPLKLPDLGL"
     gene            complement(11376..11816)
                     /locus_tag="P5313_05965"
     CDS             complement(11376..11816)
                     /locus_tag="P5313_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRY79944.1"
                     /translation="MRIQTKAYGPLDVDERQVLHFPDGLLGFEKFKDYALLDAAQKPF
                     FYLQSMDISELAFVLIDPFLFRPDYSVDADDALLSSVGIERPEDALVLSIVTVPADGS
                     PITANLMGPLIVDRASRRGVQAVMTDPRWQTKHDVLAELAAAGK"
     gene            complement(11842..13089)
                     /locus_tag="P5313_05970"
     CDS             complement(11842..13089)
                     /locus_tag="P5313_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HRY79945.1"
                     /translation="MKRVSTDQMNTDMQYWLRRTEDRTARLETKMARQTRIAELRDDP
                     LAAAHAVKYESFVSRLVRFEKNAAFAEDRYRISEGYVRQAVDIVQRVRELAVQGANGV
                     FTKQDTGYMAAEVNELLEELASLGNAQGPDGKFLFSGTEAGTQPFRLVRGFVAGAGEE
                     QIIDVQYTGNIAAREAEITQGSYAVLDQPGNEVFWAERQRVFSSREAGEYRVLEPSTI
                     RVDGRSIELRPGDTVHAIVAKINDSGAPVKASLDPARNSLVLEGTFPHQIWLEEGSGG
                     RVLTDLGILRDPDAPPPDNVAHTAQISGGSLFDAVKALRDSLYKGDVIETGGRVLGVL
                     DAGLENLNHRVAELGARTERLAATRSRLNKEIPDVTALLSGERDLDMTEAVTNLKMLE
                     YTHQASLQTAGRLLPKTLLDFLR"
     gene            complement(13095..>13611)
                     /locus_tag="P5313_05975"
     CDS             complement(13095..>13611)
                     /locus_tag="P5313_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HRY79946.1"
                     /translation="AASGEAGAFDWERPDAVLGLRTGGLDYAVAPIAHPSGWIEVTET
                     VGRNPMKVAAGFGENGKPAEIGDGSAALAIAALRDRPVMVGSLATFDDWFADAAARIG
                     LMGEQAERSAETQSRIVKNLADMRDSISGVNIDEELSNMIKFQHGYAAAARFIANFDE
                     MLDTIINRMGV"
     assembly_gap    13612..13621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13666..15129)
                     /gene="flgK"
                     /locus_tag="P5313_05980"
     CDS             complement(13666..15129)
                     /gene="flgK"
                     /locus_tag="P5313_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRY79947.1"
                     /translation="MQSTFAGIEIGKRGLVAHQQGLVTTGHNLTNASTEGYSRQRVHL
                     GTVEPIYAPQLNREETPGQIGQGVQVSRIERVKDELLEGRIVAQQGGLGYWETRDKYV
                     GLLEQVYDEPTDVSIRSRMDMFWDAWQELSLHPGELPARRAVVERGRTLIDSIHERYQ
                     RLSGIREMADQDIGIVIKQVNDLSRQIAALNVEIVKVKALGDSPNDLLDRRDLLVEKL
                     GRLVPVTTDGRDADEFMVHVDGQVLVQGKIARAFALTSGPEDDGYGRAVWADTGDEAN
                     YRGGVLGALFQLRDGDIREEIQSLDTMTLTFTDLVNEAHRAAYGLDGSTGTDFFSEYP
                     AVNNVAGNYDRNGDGQYDSTYIYRVTGVNRLDGQEQIGLAGTIRLSGPGGILEIDYRP
                     TDTVGDLVARINTSGAEVTARLTREGRLQLRATAADARENPDFVIRHLEDSGEFLAGY
                     AGILAASGGVDVVVTKDYAGRDRVVTARVGEAEAGDG"
     gene            complement(15143..15637)
                     /locus_tag="P5313_05985"
     CDS             complement(15143..15637)
                     /locus_tag="P5313_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79948.1"
                     /translation="MTTKIPALIRSVLDEAAALEKYLEERKVLSDAIRRRDWSALQDV
                     LLRIEGIALLAEATERNRSALWEEILADNGLPSDSSVYRLALGVPEPERSMLSDAYRR
                     LKVAALRARVENEALGEYVGETAGTIRSVVEELYPDQKGRIYGRSGHAVAAGVRPIVL
                     DTAL"
     gene            15741..16628
                     /locus_tag="P5313_05990"
     CDS             15741..16628
                     /locus_tag="P5313_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY79949.1"
                     /translation="MFRFINDLFWLARGVRVFALVGESGTGKSFRAKLVAQKYGIDVI
                     IDDGLLIKGDSIVAGKSAKREQLYMTAVKTALFHDKAHRDQVAKAIAKAHFKKILVIG
                     TSQKMTEKITERLQLPHPSKIIKIEDIATTEEIEKAIRTRRVEGKHVIPVPAIEVKRS
                     YPRIFYDSIRVFLQKNLGTRAISAPKVYEKSVVRPEFSKRGRVAISEAALSQMVLHCV
                     SEFNSEVRIKKITVGSDSQGYRLTLAIEVPYGTQLSGNIHHLQQYVIDNIERFTGILI
                     EEVNIVVERFGPVQEAGEV"
     gene            16724..17908
                     /locus_tag="P5313_05995"
     CDS             16724..17908
                     /locus_tag="P5313_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79950.1"
                     /translation="MKRLLAAAAAAFVLASALPAQGSNFAQARSDHYTVWAEAGQARA
                     DELAKVLEGLFTLFEEDMRFDSSRLNSRLTVRVFRDKAGFDAHLTRVVGETRSDFVYL
                     HYPTLERSELLVFEKEEPDFSASLAHQAYVQFLKAFIPNPPLWIREGFAVYYERSAWD
                     AAAGTVVFQENAAWLETLKALQNSGKLLGIEALLGISPERAESARDVFYPQSWAFVSF
                     LLDGREPRYTRFLWDSLAILAPGASLEENQGAVSALFRRWHDGKAAQEDFLAWLGSRK
                     TFAELVETGVRLYGEKNLDESEASFAEALAKNEGNYIPYYYLGLISYGRNDFDMADYY
                     YKTALQLGCDPATTNFALGLNAYAVNRLEDAKAYLAKAKEAAPDRYAARVDEILGRIG
                     TP"
     gene            complement(17961..18566)
                     /locus_tag="P5313_06000"
     CDS             complement(17961..18566)
                     /locus_tag="P5313_06000"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRY79951.1"
                     /translation="MRDGTWEARAAAAGRLCGIDEAGRGPLAGPVCAAAVILPEDFPV
                     GILADSKVLSPARREDAAATIRERARAWALGWASPWEIDRMNILAATLEAMARAYGAL
                     GTESDLVLVDGNRVPDLPAPCLAVVRGDNRVPEIMAASILAKTARDRVMDRYDWLYPE
                     YGYARHKGYPTSEHRETCLRLGPSPIQRLTFRIRIGGSGTA"
     gene            18629..19486
                     /locus_tag="P5313_06005"
     CDS             18629..19486
                     /locus_tag="P5313_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79952.1"
                     /translation="MEIRFYKLQVCGIDWLLLDRTGADPGPVPDFGNLARSLCRRRRG
                     AGGMGVIALSRPEKDIWAECFDAAGNDVPLAPCAALCAARYLFDSGRGGRESLELRTR
                     EGPARVDVIDSTRFALNLGPALSPSGSPLREGEADRAAAELDVEGRALSILPVRLAGL
                     TFAVVVAEGGLRDALAPFEEAVSAARRPDQPRMKWTPLAVRIDSRNSLAAAGRTFDAC
                     AAAGAALAAAGAYGLADREAAVRLGGDAVFVNGDADDGLYAAARPAYVFSGEYWLETG
                     PSEEGSREA"
     gene            19615..20583
                     /locus_tag="P5313_06010"
     CDS             19615..20583
                     /locus_tag="P5313_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HRY79953.1"
                     /translation="MGSSRTVMIADCDHASVDIERETLKLICREVPWQSCRTEEDVIR
                     ICSTAEGLLVQYSPLTARVLRELKRCRGIVRYGVGVDTIDLEAASDLGIVVSNVPDYG
                     TEEVSDHALALILNLVRKIYRADGLVKSGVWDFARMKPVPRLGELTLGIVGFGRIGSS
                     LARKADSLGMRIVACDPYMPPAAIPGYVSILKLEELLARADVVSVHCPLNAETRNLLS
                     AERIARMKPGAYLVNTARGGIVDEDALAEALRGGRLAGVALDVLAVEPIRPDHPLLGH
                     PDFLCTPHMAWHSSSAERDLKRKAAEEIARIVTGQTPKYQVNRRAS"
     gene            complement(20768..>21047)
                     /locus_tag="P5313_06015"
     CDS             complement(20768..>21047)
                     /locus_tag="P5313_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HRY79954.1"
                     /translation="SIVPDIRRTAELVQEISAASREQASGVDQISRAVTQLDQVIQQN
                     ASASEEMAGMAEELSGQAAQLKETMAVFRLGSDPGEHPGRGALPPPEA"
BASE COUNT         3349 a         7112 c         6911 g         3665 t
ORIGIN      
        1 gccggagccc tggcgggctt ttccgaacgc atccggtccg cctcggactc caacgaatcc
       61 ctcaagtccc tgttcgagga actcaagcgg gactcggaga gcttcctggc ggccttcaag
      121 gagatatccg gaggcaccga ggagatcgcc gccgggacgg cccagatggt cgacgccgtg
      181 caggaactgc gggtcatctc caccgagaac cgggacgcgt tcggggagat cggccgctcc
      241 gtggaatccc tggaccgcat cttcgaggag gccgcggggt tcttcgaggc cctgggccgg
      301 gacgagcgga ccatgtccgc ggccttcggg gaggccgccg gccgggtgga agccctgggc
      361 ctgcggggcg gggaaagcga acggagcttc tcggagatcg cgtcggagct ccggtacttc
      421 tccctggaca agggcgagga gaacgccgcc taccgtccgg agatcaagcg catcatcttc
      481 gaccacaagc gccgggtcgt ctcgggccgg ttgtacctgg acggccgcgt ggacgcgcgg
      541 aaccttcccg cccggagccg caccgaggac tgcctcctgg accccctgct caagaggatt
      601 tcccccaccc ttcccgaacg ggcggacatg ctgggcagac tggaccgcgc ccaccgggcc
      661 ttccacgagg cgtacaacgc cttccaggag gaatgcctgc gccccggccc ggacgaagcc
      721 ggacggcggg cccgggcctc cgccctcttc gaggaggcgg aactgcgctg gaaggccctc
      781 ttcgactacc gggaggagct caacctggta ctggagaagg cgggcggctg agagccggtc
      841 ccccgccgcg tcggacggat tcccggcgca aaaataagag gcgcccgccg atacggttgg
      901 gcgcctctct ttcggagaca cggccggaag acagatccgg cgctaggagt ttgtcgggct
      961 tgcccatcaa aaccaaagaa aaatcgcata cctgcgattt ttctacctcg caaactcccg
     1021 aggagcccgt taatcggggc tttatacgcc gataggcgta taaagcccac aagtccgacg
     1081 gactcctaga cggcggactt gcccgcctcg atggccttca ggttcatctc gataaacctg
     1141 tgcttgctgg gcgggaagaa actcttgagc acgggctcca gcttgtccag cttgaccgca
     1201 ccggtgacct tggacagggc gcccatcatc accatattgg cgatcttccc gtcccctatc
     1261 ttctccgcga tctcgttgca ggggaccttc acgaccctgc agtccttgcg cttggtctcg
     1321 gtcttcacca gggaggagtt gatgaggagg gtgcctccct gcttgacgat cccctcgcag
     1381 aggggcaggg agtcctcgtt catcaccacc gcgctgtccg gagtggtcac gagggggctg
     1441 gcgatctccg tggtggagac cacgcagccc gcccgggcga tgccgccgcg cttctcggct
     1501 ccgtagaagg ggaacatggt gacttcgagg ccttccttca tgccgcagta gacccagagc
     1561 tggcccatgg agatgatgcc ctggccgccg aacccggcca tgaaactctt ttccgtcatt
     1621 tggatgcctc cagggtgttc ttgacctccc cgagctggta gaaggggatc atgttcttct
     1681 ccagccactc cacgctctcc tggggatcca tgctccagtt ggtcgggcac tgggagagga
     1741 tctccaccat cgtgaagccc acaccgctca tctgggcctc gaaggccttc ttgatgtact
     1801 gcttggtctt acggacgttg gccgcgttgt tcacggaacc ccgggccagg tagcgggtgc
     1861 cctccagggt cgccagcatc tccaggacgt ggatgggata gcccatcccg gcgccctcgg
     1921 ggtcccggcc gtagggggcg gtggtggcct tctgacccac cagggtcgtg ggggccatct
     1981 gcccgccggt catgccgtag atggcgttgt tcacgaagat ggtggtgaac ttctcgcccc
     2041 ggttggcggc gtggacgatc tccgcggtgc cgatggcggc caggtcgccg tcgccctggt
     2101 agcagatcac caggtggtcc gggcgcaccc gcttgacgcc ggtggccgcc gcgggggtcc
     2161 ggccgtgggc cggctggacg gagtcgatgt cgaagtagag gtccgcccag atggagcagc
     2221 ccaccgggtt ggtgatgatg ctcttctcct ggaggcccag ctcgtcgatc acctcgcaga
     2281 tgatgcggtg gatcaccccg tgtccgcagc cggggcagta cttggtctgt atgggtttca
     2341 ggctcttggg atgcccgtac gcgagttcca tcatatcctc ctattttccc agaagcttgc
     2401 gggcttccgc caggaccgct tcctcggtgg ggatgttgcc cccgcagcgg ccgtagaacg
     2461 agacctgcgc cttgccggcg accgcgagct ggacgtcctg gaccatctgt cccatgctca
     2521 tctccaccgt caggaagcgc ttgcccttgg cggccagggc cgcgatccgg tcatagggga
     2581 agggccagag ggagatgggc cggaagatgc ccagctcgat ccccagggcc ttggccatct
     2641 gctgggcccc caggcagacg cggctggagg tcccgtaggc caccaggatc aggtccgcct
     2701 tgtcggtgtt gacctcctcg aaccggatct ccttcttctt gatctgttcg tagcgcagga
     2761 agcgctcgtg gaccttggcc tccagctcgt cggggaccag gttgatggag gagacgtggt
     2821 tgatgggccg gtgggtcttg tactggccgc cggcggccca gggccgggcc gggagctttt
     2881 ccaggtcccg gggctccggg aaggtcacgc cctccatcat ctgcccgatc atcccgtccg
     2941 ccaggatcat gacgggcatc cgccactggt cggccaggtc gaaggcttcg aaggtcaact
     3001 ccgcagcctc ctggacgctc ttgggggcca ggaccaggtg gaagtagtcc ccgtgtccgc
     3061 cgccccgggt ggcctggaaa tagtcgccct ggccggggga gatccccccg aggcccgggc
     3121 ccgagcgggc cacgttcacg accacgcagg ggacgtcggc tccgcaggcg taggagatcc
     3181 cctcctgctt gaggctgatc ccggggctgg aggagctggt catggcccgg gctcccgcgg
     3241 ccgcggcccc gaagaccatg ttgatcgcgg aaacctcgct ctccgcctgg atgaaggtcc
     3301 gcttgtggtc gatcatgtgg cgggccatgt aggcggtcag ctcgttctgg ggggtgatgg
     3361 ggtacccgaa ataggcagtg cacccggcgc ggatcgcggc ctctccgatg gcgtcgttgc
     3421 ccttcatcaa tcgcttttct tcggccatgt tccgtgcctc cttacagctt gtagaccgtg
     3481 acggccacgt ccgggcagac gaggtagcag ttgccgcagg ctatgcattt gtccggcgcc
     3541 gccgcccggg cgggatagta gccccaggag ttcggagccg cgtccgcctc cagcaccttg
     3601 gtcgggcagg cgcgcacgca gagcatgcac cccttgcatc gttcccggtc tatctccggt
     3661 tttcccttga tcgccataga tactcctttc ggtggacggt ggacagcgga cggaaggatg
     3721 gaaatcctct ccacggttca ccgttcactg ttcactgttc acttcccgtc gccaccacgc
     3781 aggccagggc aatgctggcc agtttcacgt ccgccgtgtc ggcccggctg gtcagcacca
     3841 ccggcctggc cgctcccacc acgatcccgg cccccttggc ctgacccagg tccagtaggc
     3901 acttgtacag gatgttcccc gcctcgatgt ccggggccac gaagatgtcc gcgtccccgg
     3961 ccacctcgga cacgatgccc ttgatacggg cccgctccgc gctgaccgcg ttgtccaggg
     4021 ccagggggcc gtccaccagg cagcccttga tctgcccccg ccggttcatc tgggtcagga
     4081 ccgccgcgtc ggcggtgcag ggcatcttct cccagttcac cttctccacc gccgccaaga
     4141 gcgccacctt gggcaccgga atccccaggg cctgggccac ctggaccgcg ttggcgatga
     4201 tgtcgatctt gccgggaatg tccggggcta tgttgatggc tgcgtcggtg acgaacagga
     4261 gcttgtggta ggtctgggtt tccatcaccg ccacgtggct ggtcaagcgg cccgtgttca
     4321 ggccgttttc cttgttcagc accgccttca gcaggaccga ggtgtctagg atgcccttca
     4381 tgaggaggtc cgcacggcct tcccggacga gttccacggc ccgggccgcg gccttcccgc
     4441 tgtccttctc ctcgatgcgc tggaaccggg acacgtcgag gccgagctcc ccggcgatgc
     4501 ggtccaccgc cgccaggtcc ccgatcagga tggggtccgc caccccgtgt tccgccgccg
     4561 cgcaggccgc ctccaaggcg ctgtgctcct gagccgccgc cacggcgatc cgcttgcgcc
     4621 ccagggaccg ggccctgtcc agtatctcct gaatggtctt catctcaata ctcctggata
     4681 tgagcttccc cggacagggc ccggctgccc gccagggcca aggcttccag ctcgccctct
     4741 ccgggataca cgacgaccgg ggcgatccaa ccgcacagcc cggcgatgcc ttcctggatc
     4801 cccttgccgt gggcgatccc gccggtcagg atgatcgcgt ccaccttgcc ggccgcggcc
     4861 gcggccatcg agcctatgaa ctttcccacg ttgtagatga aggcgcggta atagaggtcc
     4921 gccttgggat cccccgcctg gacggccttc tcgacctccc gcatgtcgtt ggtgcccagt
     4981 aggctgacca tgcccccctg ccccgtgatc atcttgagga cttccttctc cgagtgcttg
     5041 cccgagaagc agagcttggc caggtccccg gccgggaggg ttcccgagcg ttcgggactg
     5101 aaggggccct ccccgttcag ggcctggttc acgtccgtca cccggcccct ccggtgcaga
     5161 ccgacggaga caccgccccc catgtgggcc acgatcaggg tcaggtcctc gtaggcgcgg
     5221 ccggattccc gggcgtacct gcggcagacg gctttctggt tcagggcgtg gaagatgctg
     5281 cggcgctgga agagggcgtg ccccgccaca cgggcgacgt cggagagttc gtccaccacc
     5341 acggggtccg cgatgaagga agggaccccc cactcccggc ccagggcgtc ggcgatgagg
     5401 gcaccgaggt tggaggcgtg ttccccgtac ttggccgccc gaaggtcctc cagcatgggg
     5461 ctcgagacgc ggtacacccc gcccggtatg ggtcggagca gtcctccgcg gccgaccacc
     5521 gcgtcgatgc cgtgcgcgtc gaaaccgcgc ttccgaaggg cgtcccggat gtgctgttcc
     5581 cggaagccgt actggtcggc tatggaagcg taccggccga tctcctcggc cgaatggttc
     5641 accgtttccg tgaagacctc ctcgagatcc cggaagacgc ctatcttcgt ggaagtggat
     5701 cccggattga tcacaagaat ggttgccatg gacgctcctt agtcccggat atccgaaggc
     5761 ccgcgctggc cgtcaggagg aggttgtttc gatgccggtc ggcatgggtt ccgtcggatg
     5821 cttgagtata cccgtgaatt ccggagtgcg caagaaaaaa ctgcaattgt tgaaaggacg
     5881 tttcgttgat caaaatactt attcctatgt ttgaatagaa tgggtcttgg tgctgcccgg
     5941 gcgggagtat tgcccgggca gcttaggcga ttgatggaag caggaagggt ggaaccgtcg
     6001 gaactaccgg gttttgagaa gccggaccat gtagaggcgg atatactcct ccagggccga
     6061 gtggttccgg atcttgagca gggagggggt atccgccagg gtcgcggcca ggcccttgac
     6121 ccgctcggcg gtgaaattcg aaggcttcag ggagcggagc agcccccgca ggtaatcccg
     6181 gaccagggag ttgacgtcct cggtcaggtc cgccttggcc tgggggttca gcatggtgtt
     6241 ccatcggttg atcaggaccg agaggtactc gtccagcttg tacccctccg gcaggaggac
     6301 ctgctcgacc ttggccgccg ccgcggcgaa ctcgatcctc cggtcggaga atttcgcgga
     6361 ggtccccgtc ttctcggtct tcggctccga ggctttctcc gcgatgctgg ccagccgctc
     6421 cttccgggcc cgcttccggc tggtcagccg gaacaggaag gcgaagagcc cggagaggat
     6481 ggggagggtg taccagatgg gcaggagatt gcggacatcc gtcaccatgt gcttccgccg
     6541 caggtccaga agttcgtcga tcgggaccag gtcccccttg gagaagaacc ggtccagctc
     6601 cgggaccgcg tccggggtct cccggagttc gttgtagacc agggagaata tgcgctccga
     6661 gatcaggacg aagaggatcg gatacccctt ggcgacccgt tcctccaggt cccggcggta
     6721 ggcggcgtcg tcctccatgg tgggcgtcga ggcgtagacc tccagcaccg acttccactc
     6781 ggcgatcagg gaggtccgga tcgaggcccg ggcctctccc aggagacgga ccaccagggg
     6841 caggacccgg tccttggcga ccagccaccg ctggccccgg gacgtgacgg gcatcaggat
     6901 ctccgggagg cgtccggtct ccgcccgggt ggtgcgctcc cggatccact cctccagctc
     6961 ctcccgggtg tattttccca ggaggggacg ccccatggaa tcccggaagt tcgtcacctc
     7021 gtccagggtg aagttgtagg gggatttgcg gagcagggtg tccagggtct tgaaggccgt
     7081 ctcccgctcg accgcccgct gcgccttccc tttatagaag ttgttgtaca cctcgcagag
     7141 gtagatggcc tggagatagg cccaatcctc cgggttcttg tccgatttct tgaagacatc
     7201 ctgcttcatg tagctggcca ggtaggccca gagggcgaag gtgaagtcgt tcccggaggt
     7261 cctcatgtcc cgaaccgcgt cgaagggctt gatcaggatg gtggagaggg actccttgag
     7321 ctgcatctcc ttgcccggaa aggcgtagga cagcttgttc tggacgaagt ccttgttccc
     7381 ccccttgcgg aggtactggc ggaccttcag gaccgcgtgc tccaggacct tgtccgccgc
     7441 catcggggac aggatcagca cggggcgtat gccctccgga aagaccagcc gatagaaggg
     7501 gaccgcctgg acccattcgg ccgggagctc cgcgtggatg ctctcgtcgt ccgggaaggg
     7561 gatgtcggga ttctcctcca tccggcggta ttccgccagg aggaggtccg tgaagtgctc
     7621 cggcagggag atggattcga tctgcccgcc cacggtgttg atggataggt tgcggtcccg
     7681 ggcgtactgg aagagcttcg ggaccaggac gacatccgga ttggaggcca ggtcccggta
     7741 tatcggttcc ggacggttgg cctgagcggc ctgccgttgg accatctgcg cgaaggaacg
     7801 gtagtctatg gtcgagacct tgttcttcgt ggcgtaggag cgtagaagct gaagtacgtc
     7861 gatatcacgg gccatagggt atactacccg cctccgaagg atcgggcaac aaaaaacggc
     7921 cgatcccagc gggaacggcc gcttctcccg cgacgcgtca acgggtcttg tgccggttct
     7981 ttctcttctt cttcttgcgc ttatgcgtcg cgatcttctt gagctttcgc tttcttccgc
     8041 agggcacggt cgcactcctt ctatgagctg ggtcctacgc acggcccgcc ggaggccgcc
     8101 gggactccgt accgcgtaga cgtgtccgca agtatgcgaa aaagcggtcc ggccgtcaag
     8161 gggcccgggc accgcttttc ccttcccgcg ccttgcaaac ttgacggcca tggattatac
     8221 agtagcaagg tatccgggct gtccaagagg ctcgatcggc ttattggtca gctggttccg
     8281 atgcggcggt atccgccacc cgcggaccgg ggaaaaggag cttggacgcg tatgatagcc
     8341 aagagcgaca tgccgaatat caacgaaaag cgccccgacg gagtcaaacc cgacggatcc
     8401 ccctatcgcg tcctgatcgt ggacgattcc atgttcatcg ccaaacagct ggggcagatc
     8461 ctgacctccg agggcttcga cgtggtgggc acggcggggg acggagtcgc gggcatcgaa
     8521 aaatacaagg aactgtatcc gaacgtggac ctggtcacca tggacatcac catgcccaag
     8581 atggacggcg tcacggccct ggaaaagata ctggaattcg acaagaacgc ggcggtggtg
     8641 atggtaagcg ccctgggcaa ggaggacatc gtcaagaagt ccctcctgct gggagccaag
     8701 agctatatcg tcaagcccct ggaccggaag aaagtgctgg aacggatcct gacgatcctg
     8761 ggcaggtgaa gccggccgct ccccgccggg atcaatcctc gacgctctcc ccgcccttct
     8821 cggaatcctt cttgcggacc accatctttc tccggggtcc ttccttggcg gaatccccgt
     8881 ccggccggac cgcgggcctg gctgcgcgcc ggggcttttt gggcgcctgg gaggtgtcga
     8941 tgtaccgcag ggccttctcg aaagcctgga ggaactcccg catgtcttcc ttgaagacca
     9001 cgatggacct gcggtcgaac tccgcgttct ccgtgggctt ggactccacg atgttcagga
     9061 acacgtcccc ggtccggttt tccttgacgt tgaagaaata ggatctgccc ctgcagttga
     9121 tcttgctgga gaacaactca ccccgaatgc ccatgcacgc tccttttgcg cccgttcgcg
     9181 gatagtagca gataatggag gcgggcgcaa gacaggagcc ctggctcctg ttacctgact
     9241 cctgaatcct tcaccatttc cttcagccat tccaggagcc gggtctcgcc ctcgggatcc
     9301 ctgcctcgga ggtccgccat gatgcggagg accggctccg tgccgctgcc ccgcatccag
     9361 aggaaccccg cgggcagacc cttcccgtcc cggaaaagga tccgcagccc cccccgcccg
     9421 gcggccccga agtccccggt cgcgtcccgt tcccccgagc ccgccgaagc ccgggcctcc
     9481 caggatacga agccgaagct ccgggcgaac tccccgcggc gggcttccca gtcttcccgg
     9541 aaccgggcct ggaatcgtcg cttcagctcc gccggatccc ccgcggagat gcgcagcagg
     9601 gcccgttcct cgaagacgct ggtggtcgtc caggccggca ggctggaaag gacatcgtcc
     9661 agggtgaagt ccggggaata ctccccgccc cggcccgaag cctccatcca gacccggaag
     9721 ggggccggcc cgcccccggt tccccggagg gccaggagct tgaccagggc cgtcacggtg
     9781 ttcaggggat cccggaccgc cgcgggatgg gtgatgttcc ctccgttcga cccctccccc
     9841 aggattcgga ccgtccaacc ctcggcccgg agggcccggg ccaggttcac gacgttggct
     9901 tcccccgtct ccgcccggaa gacctcggct cccagggtcc gggcgatctc gtcgatccgg
     9961 aggctggtcg cgtcgttcac ggccaccgcg accttgccgg aaccctcgcc tccggctccg
    10021 cggaggaggg ccaactcgga aaggacggca agggagaaga cctcctgggc ctccagggcc
    10081 tccgcaccgc ctgaggcatc tgaccagcgg accaggtttc cccggtctcc gtcgcagtcc
    10141 ggcacgtagc ccaggacaaa ggccggatcc tgggcccggg ccgcttccag ttccttccgg
    10201 cagaggtcca gggaggctcc ctccgggacg atgcggtggg caaaggcccg gggcctggcg
    10261 ttcacgagac ggggccgggc acccagcatg gaaaggtagt ccgcgtcgat ggacagggag
    10321 cgggcggacc cgttcatctc cgccacgatc cccagggggc gggactcgca ggcccgggcc
    10381 agatcgtcca ggacccgctc ctgctcgtcg aggtcttccc ggtccgcggc tgtccgccgg
    10441 gcgaagaggg tataggcgga gactgcggac ctcttctccg cggagcaggc ggagaagacc
    10501 cgggccaccg cccgctcgtc gaccttggag gccgcctcca ggacggagcg ccgggcggcc
    10561 tgatcccggg ctaaagtccg gaaccgtccg gccagggcgt ccgcctcgga ggccggaagg
    10621 acccccccgt ccgcgaagcc gaacttgacc ccgttgtgtc cggggggatt gtggctggcg
    10681 gagatgtagc agaatccgga aagcccggaa ccctcccggc gggtccaagc catgatctcc
    10741 ggggccgcga tgatgaaggc gtacttcggg acgatcccgg aggccaggaa gacccggacc
    10801 atgacgtccg ccagggcggg gcccgtgggc cgggagtcga tccccagggc cagggccggc
    10861 gccgttcccg ggctcgccgc gtccgccagg cgctccgcga aaacccgggc catgcaggcg
    10921 gccaggcaga ggtccgcggg acgtacggcg ggggtaaggg actcctcctc gccgtccccg
    10981 gcgaagacct tgcgccaccc cgaggcggac aggatcaggg aaccgagggc ggcttcgagg
    11041 tccgcgtccg agggctcccg ggacgggtcc aggggacgga gggagggatc tcccacggga
    11101 aagcccgtgg agggatgaag tatgagggcc atgggaccgg attatagcac ggaaggggtc
    11161 agagaccgag gtccgggagc ttgaggggac tctcggcggc ccgcaggttc tcctgctgga
    11221 tcgagtcgaa gacttcctgg cggtagacct tcacgttccg cggcgcgtcg atgcccagct
    11281 tgacctggtc accccgaacc tccacgacgg agatcacgac gtcctccccg atgacgatcc
    11341 gctccccgag cctgcgggac aggatgagca tgccgctact tcccggccgc cgccagctcg
    11401 gccagcacgt cgtgcttggt ctgccagcgg ggatcggtca tgacggcttg gactccgcgc
    11461 cggctggccc ggtccacgat caggggaccc atgaggttgg ccgtgatcgg ggatccgtcc
    11521 gcggggaccg tcacgatgga caggaccagg gcgtcctcgg gccgctcgat ccccaccgag
    11581 gacagcaggg cgtcgtcggc gtccacggaa tagtcgggcc ggaacaggaa gggatcgatc
    11641 aggacgaagg cgagctcgga gatgtccatc gattggaggt agaagaaggg cttctgcgcg
    11701 gcgtccagga gggcgtagtc cttgaacttc tcgaagccca ggaggccgtc cgggaagtgg
    11761 aggacctgcc gctcgtcgac gtccagggga ccgtaggctt tggtctgtat tcggatcatg
    11821 acggtgccag tctccccttt cctagcgcag gaagtcaagg agggtcttgg gaagcaaacg
    11881 gcccgcggtc tgcagggacg cctgatgggt gtattccagc atctttaggt tcgtgacggc
    11941 ctcggtcatg tccaggtccc gttcccccga cagcagggcc gtgacgtccg ggatctcctt
    12001 gttgagccgg gaccgggtgg cggccaggcg ctcggtccgg gctcccagct cggccacccg
    12061 gtggttgagg ttttccaggc ccgcgtccag gactcccagg accctccccc cggtctcgat
    12121 cacgtccccc ttgtacaggg aatcccgcag ggccttcacg gcgtcgaaca gggacccccc
    12181 ggagatctgg gccgtgtggg ccacgttgtc cgggggaggc gcgtcgggat cccgcaggat
    12241 ccccaggtcc gtgagtaccc ggcccccgct cccctcctcc agccagatct ggtgggggaa
    12301 ggtcccttcc aggaccaggg agttccgggc cggatccagg gaagccttca ccggggcccc
    12361 ggagtcgttg atcttggcta cgatggcgtg caccgtgtcc ccgggccgga gctcgatgga
    12421 gcgcccgtcc acccgtatcg tggagggttc caggacccgg tactcgccgg cctctcggga
    12481 ggagaacacc ctctggcgct ccgcccagaa gacttcgttg ccgggctggt ccaggacggc
    12541 gtaggaaccc tgggtgatct ccgcctcccg tgcggctatg ttgcccgtgt attggacgtc
    12601 gatgatctgt tcttccccgg cgcccgccac gaagccccgg accaggcgga agggctgggt
    12661 ccccgcctcg gtcccggaaa aaaggaattt cccgtccggt ccctgggcgt tgccgaggga
    12721 ggccagctcc tccaggagct cgttcacctc cgcggccatg tagcccgtgt cctgtttggt
    12781 gaagacgccg ttggcgccct ggaccgccag ttcccggacc cgctggacga tgtccaccgc
    12841 ctgccggaca tagccctcgg agattcggta ccggtcctcc gcgaaggccg cgttcttctc
    12901 gaagcggacc agccggctca cgaaggactc gtacttcacc gcgtgggccg cggccagggg
    12961 gtcgtcccgg agctccgcga tgcgggtctg ccgggccatc ttggtctcca ggcgcgcggt
    13021 ccggtcctcg gtcctgcgga gccagtactg catgtctgtg ttcatctggt ccgtgctgac
    13081 gcgtttcatc gggcttacac tcccatccgg ttgatgatgg tatccagcat ctcgtcgaag
    13141 ttggcgatga accgggccgc cgcggcgtag ccgtgctgga acttgatcat gttggaaagc
    13201 tcctcgtcga tgttgacgcc ggagatcgag tcccgcatgt ccgccaggtt cttcacgatc
    13261 cgggactggg tttccgcgga gcgctccgcc tgctcgccca tgagaccgat ccgggccgcc
    13321 gcgtcggcga accagtcgtc gaaggtcgcc agggagccca ccatgacggg ccggtcccgc
    13381 agggccgcga tggccagggc ggcgctgccg tccccgatct cggcgggctt gccgttctcc
    13441 ccgaagcccg cggccacctt catggggttg cggcccacgg tctcggtcac ctcgatccat
    13501 cccgacggat gggcgatggg agccaccgcg tagtccaggc ccccggtccg gaggcccagg
    13561 acggcgtccg ggcgctccca gtcgaaggct ccggcctccc ccgaggcggc cnnnnnnnnn
    13621 ncggccagtt cgccctccag gcgggtgggc ccctcgatga cgaacctatc catcgcccgc
    13681 ctccgcttcg cccacgcggg cggtcaccac gcggtcgcgt cccgcgtaat ccttcgtcac
    13741 cacgacatcc acgccgcccg aggcggccag gatcccggcg tagcccgcca ggaactcccc
    13801 cgaatcctcc aggtgccgga tcacgaagtc cggattctcc cgggcgtccg ccgcggtggc
    13861 ccgcagctgg aggcgcccct cccgggtcag ccgggccgtc acctccgccc cggaggtgtt
    13921 tatccgggcc accaggtccc ccacggtgtc cgtgggccgg tagtcgatct ccaggatgcc
    13981 cccggggccg gaaaggcgga tcgtgcccgc cagccctatc tgctcctgcc cgtccagccg
    14041 gttcaccccg gtgacgcggt agatgtaggt ggaatcgtac tgtccgtccc cgttccggtc
    14101 gtagttgccc gcgacgttgt tgaccgcggg atactcggag aagaagtccg tgcccgtcga
    14161 cccgtccagg ccgtaggccg cccggtgggc ctcgttcacc agatccgtga aggtcagggt
    14221 catggtgtcc agggactgga tctcctcccg gatatccccg tcccgcagct ggaagagggc
    14281 ccccaggacg cccccgcggt agttcgcctc gtcccccgtg tccgcccaga ccgcgcggcc
    14341 gtagccgtcg tcctcgggcc ccgaggtcag cgcgaaggcc cgggcgatct ttccctgcac
    14401 caggacctgg ccgtccacgt ggaccatgaa ctcgtccgcg tcccggccgt cggtggtcac
    14461 ggggaccagt ctgccgagct tttccaccag gaggtcccgg cggtccagga ggtcgttggg
    14521 gctgtccccc agggccttca ccttgacgat ttccacgttg agggccgcga tctgccggga
    14581 caggtcgttc acctgcttga tcacgatccc gatgtcctgg tcggccatct cccggatgcc
    14641 ggacaggcgc tggtaccgct cgtggatcga gtcgatcagg gtccggcccc gctccaccac
    14701 ggccctgcgg gcgggaagct cgccgggatg cagggagagc tcctgccagg cgtcccagaa
    14761 catgtccatg cgggagcgga tggagacgtc cgtgggctcg tcgtagacct gctccagcag
    14821 gcccacgtac ttgtcccggg tctcccagta acccaggccg ccctgctggg ctacgatccg
    14881 gccctccagg agctcgtcct tgacccgctc gatgcggctt acctggacgc cctggccgat
    14941 ctggccgggg gtctcctcgc ggttcagctg gggggcgtag atgggctcca cggtgcccag
    15001 gtgcacccgc tggcgggagt acccttccgt ggaggcgttg gtcaggttgt ggcccgtggt
    15061 caccaggccc tgctgatggg ccacgaggcc ccgtttacct atctcaatgc ccgcgaacgt
    15121 agactgcatt ccgtcccccc gcctacaacg ccgtgtccag gacgatgggc cggacgcccg
    15181 ccgccacggc gtgccccgag cggccgtaga tccggccctt ctggtccgga tagagctcct
    15241 ccaccacgga ccgtatcgtg cccgcggtct cgcctacgta ttcgcccagg gcttcgtttt
    15301 ccacccgggc gcgcagggcc gccactttca accgtcggta cgcgtccgag agcatgctcc
    15361 gttcaggttc cggcacgccc agggccagcc ggtacaccga ggagtccgag ggaagcccgt
    15421 tgtccgccag gatctcctcc cagagggcgg agcggttccg ctcggtggcc tccgccagga
    15481 gggctatgcc ttcgatccgc agcaggacgt cctgcagggc ggaccaatcc ctgcggcgaa
    15541 tcgcgtcgga caggaccttg cgctcttcca ggtacttctc gagcgcggcg gcctcgtcga
    15601 ggacggatcg gatcagcgcg ggaatcttcg tcgtcatagg gcgctccttc tctacgaata
    15661 tcggaaggcc gagccggcgg tttagaggat tccggcgccg gcttgtctgc ataggcggcg
    15721 gcggggtagt attcgggacg atgttccgat tcatcaacga cctcttctgg ctagcccggg
    15781 gagtccgggt cttcgccttg gtgggcgaga gcggcacggg caagagcttc cgggccaagc
    15841 tggtggccca gaagtacggc atcgacgtca tcatcgacga cggcctattg atcaagggag
    15901 actccatcgt cgcaggcaag agcgccaagc gggaacagct gtacatgacc gccgtcaaga
    15961 ccgccctctt tcacgacaag gcccaccggg accaggtggc caaggccatc gccaaggcgc
    16021 atttcaagaa gatcctggtc atcgggacct cccagaagat gacggagaag atcaccgagc
    16081 gcctccagct cccccacccc tccaagatca tcaagatcga ggacatcgcc acgacggagg
    16141 agatcgagaa ggccatccgg acccggcggg tggaggggaa gcacgtcatt cccgtgcccg
    16201 ccatcgaggt gaagcgcagc tacccccgga tcttctacga ctccatacgg gtcttcctgc
    16261 agaagaacct gggcacccgg gccatctcgg cccccaaggt ctacgagaag tccgtggtcc
    16321 gtcccgagtt ctccaagcgg ggccgggtgg ccatctccga ggcggccctg tcccagatgg
    16381 tcctgcactg cgtgagcgaa ttcaattcgg aggtccggat caagaagatc accgtgggct
    16441 ccgactccca gggctaccgg cttaccctgg ccatcgaggt cccctacggc acccagcttt
    16501 ccggcaacat ccaccacctc cagcagtacg tgatcgacaa catcgaacgc ttcacgggca
    16561 tccttatcga ggaggtgaac atcgtggtgg agaggttcgg tccggtgcag gaggcggggg
    16621 aggtttgaca aaccgccttg cgggttctag gattagggcg attccaagat gctccggcat
    16681 tttgattgac ggaagtacgt ccacgcgata tccgggagga atcatgaaaa ggcttctggc
    16741 cgcggccgcc gcggcattcg tattggcctc ggcccttccc gcccagggat ccaatttcgc
    16801 ccaggcccgg tccgaccatt acacggtctg ggcggaagcc ggccaggccc gggcggacga
    16861 actggccaag gtcctggaag gcctgttcac cctcttcgag gaggacatgc gcttcgactc
    16921 gagccgcctg aacagccggc tgaccgtccg ggtattccgg gacaaggcgg gcttcgacgc
    16981 ccacctgacc cgcgtcgtcg gcgaaacgag gtccgatttc gtctacctcc actaccccac
    17041 cctcgagcgg tccgagctcc tggtcttcga gaaggaggag ccggatttct ccgcctccct
    17101 ggcccaccag gcctacgtcc agttcctcaa ggccttcatc ccgaatcccc ccctgtggat
    17161 ccgggagggc ttcgcggttt attacgaacg ctccgcctgg gacgcggccg ccggaaccgt
    17221 ggtgttccag gagaacgcgg cctggctgga gaccctgaag gccctccaga attcgggcaa
    17281 gctcctcggc atcgaggccc tcctgggcat cagccccgag cgggcggaga gcgcccggga
    17341 cgtcttctac ccccagtcct gggccttcgt cagtttcctg ctggacggaa gggagccccg
    17401 gtacacccgg ttcctctggg attccctggc catcctggcc ccgggggcga gcctggagga
    17461 gaaccagggc gccgtatccg ccctgttccg ccgctggcac gacggcaagg ccgcccagga
    17521 ggatttcctg gcctggctgg gttcccgaaa gaccttcgcg gaactggtgg agaccggggt
    17581 ccggctctac ggggagaaga acctggacga gtccgaggcc tccttcgcgg aagccctggc
    17641 caagaacgag ggcaactaca taccgtatta ctacctgggc ctgatttcct acggtcggaa
    17701 cgacttcgac atggcggact actactacaa gaccgccctc cagctgggct gcgatccggc
    17761 cacgacgaac ttcgccctgg gcctgaacgc ctacgccgtg aaccgcctgg aggacgccaa
    17821 ggcctatctg gccaaggcca aggaggccgc cccggaccgc tacgcagccc gggtggacga
    17881 gatcctcggg cggatcggca caccctagcc gacccgtcgt gatcaccggg gatccggggc
    17941 gctaggagtt tgtcgggctc ctaggccgtc ccggatccgc cgatcctgat ccggaacgtc
    18001 agccgctgga tgggggaagg cccaagcctc agacaggtct cccgatgctc cgaggtcgga
    18061 tagcccttgt gccgggcata cccgtactcg ggatagagcc agtcgtaccg atccatgacc
    18121 cggtcccggg ccgtcttggc caggatggac gcggccatga tctccggaac ccggttgtcc
    18181 ccccggacca cggccaggca gggcgcgggc agatcgggga cccggttgcc gtccacgaga
    18241 accaggtccg actccgtccc gagggctccg taggcccggg ccatggcttc tagggtcgcg
    18301 gccaggatgt tcatccggtc gatctcccag ggactcgccc agcccagggc ccaggcccgg
    18361 gcccgttccc ggatcgtcgc cgcggcgtcc tccctgcggg ccggggacag gaccttggag
    18421 tccgccagga tcccgacggg aaagtcctcc ggcaggatga ccgcggcggc gcacacgggc
    18481 cccgccaggg ggccgcggcc cgcctcgtcg attccgcaga gtctcccggc cgcggccgcc
    18541 cgggcctccc acgtcccatc ccgcatgggg ccactctaat tcccgaatgc ccctttcgca
    18601 aggccggcaa agcggataga gtttccccat ggaaatccgg ttctacaagc ttcaggtgtg
    18661 cgggatcgac tggctcctcc tggaccgcac gggcgcggac ccgggtcccg tcccggattt
    18721 cggaaacctg gcgcgttccc tgtgccgcag gcgccgggga gccggaggca tgggagtcat
    18781 cgccctctcc cggcccgaga aggacatctg ggcggaatgt ttcgacgccg ccgggaacga
    18841 cgtccccctg gcgccctgcg cggccctctg cgccgcccgg tacctcttcg attccggacg
    18901 cggcggacgg gaatccctgg aactccggac ccgggagggc cccgcgcggg tggacgtcat
    18961 cgactccacc cggttcgccc tgaacctggg ccctgccctc tcgccttcgg gatcccccct
    19021 gcgggagggc gaggcggacc gggccgcggc ggaactggac gtcgagggca gggccctctc
    19081 catcctgccc gtccgcctgg ccggcctgac cttcgcggtg gtcgtggcgg aaggcggcct
    19141 ccgggacgcc ctggcgccct tcgaggaagc cgtttcggcc gcccgccgtc cggaccagcc
    19201 ccgcatgaag tggacgcccc tggcggtgcg gatcgactcc cggaacagcc tcgcggccgc
    19261 gggaaggacc ttcgacgcct gcgccgccgc gggcgccgcc ctggccgccg cgggggccta
    19321 cggactcgcg gaccgggaag cggcggtccg cctggggggg gacgccgtgt tcgtgaacgg
    19381 ggacgcggac gacggcctgt acgcggccgc ccggcccgcg tacgtgttct ccggggagta
    19441 ctggctggag accggccctt ccgaggaagg ctcccgggag gcttgacccg ccccgcaggg
    19501 actcaggaac ccccgaaaaa ccgccgatag gcttagaacg gcgccctcga agcttccgat
    19561 ccggccgcgg cgtatgtccc cgcgccggtc cgggaaggcg cccgggaggt ctccatgggt
    19621 tccagccgga ccgtcatgat cgcggactgc gaccacgcca gcgtggacat cgaacgcgaa
    19681 accctaaagc tcatctgccg ggaggtaccc tggcagtcct gtcgcaccga ggaggacgtc
    19741 atccggatct gctccaccgc ggagggtctg ctcgtccagt actcgcccct gaccgcccgg
    19801 gtccttcggg aactgaagcg ctgccggggc atcgtccggt acggggtcgg ggtggacacc
    19861 atcgacctag aagcggcctc ggacctgggt atcgtggtca gcaacgtgcc ggattatggg
    19921 accgaggagg tctcggatca cgccttggcc ttgatcctga acctcgtgcg gaagatctac
    19981 cgggccgacg gcctggtcaa gagcggggtc tgggatttcg cccgcatgaa acccgtcccc
    20041 cgcctggggg agctgaccct ggggatagtg ggcttcggcc gcatcgggag ttccctggcc
    20101 cgcaaggccg attccctggg catgcgcatc gtggcctgcg acccctacat gcccccggcc
    20161 gccatccccg gctacgtctc catcctgaag ctggaagagc ttctggcccg tgccgacgtg
    20221 gtctcggtcc actgccccct gaacgcggaa acccgcaatc tgctctccgc ggagagaatc
    20281 gcccggatga aacccggcgc ctacctcgtg aacaccgccc ggggaggcat tgtggacgag
    20341 gacgccctcg cggaagccct ccgggggggg cgcctggccg gggtggccct ggatgtcctg
    20401 gccgttgagc ccatccgccc cgaccatccc ctcctggggc acccggattt cctgtgcact
    20461 ccccacatgg cttggcattc ctcctccgcg gaacgggatc tcaagcgtaa ggccgccgag
    20521 gagatagccc gcatcgtcac cggacagact ccgaagtacc aggtcaaccg cagggcttcc
    20581 tagcaggttg ttgaaatact cgaggcattt caacaacctg ctatccattg ctcgaggacc
    20641 ttacggttct atgcgcaatt gcgatattaa ggtagtgatg aaaaaggctc tatagccttt
    20701 ttcatcaccg tgctagagca ccgcagggtt accgaccggt gatcgacccg gaaccgttca
    20761 cctcccgtca cgcctccggg ggaggcaggg cgccccgccc cggatgttcc ccggggtccg
    20821 agccaagtcg gaataccgcc atcgtctcct tgagttgggc cgcctgcccg gagagttcct
    20881 cggccatgcc ggccatctcc tcgctggcgc tggcgttctg ttggatcacc tgatccagct
    20941 gcgtaaccgc ccgggatatc tggtcgaccc cggaagcctg ctcccggctc gcggcgctga
    21001 tttcctggac cagttcggcg gtcctgcgga tgtccggaac gatgctt
//
LOCUS       DAOTEI010000042        20919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1667_length_20919_cov_5.438986, whole genome shotgun sequence.
ACCESSION   DAOTEI010000042 DAOTEI010000000
VERSION     DAOTEI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 20919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20919
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1667_length_20919_cov_5.438986"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="P5313_06020"
     CDS             complement(<1..753)
                     /locus_tag="P5313_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY79955.1"
                     /translation="MINYVTGEDIEALAKSGNSRLVLGPNDRLTGIARENCERYGISI
                     VAPGSDTSAASPRSGAGSHASPAASFVPESFRPSSAPARVPRAGSGGSGPEGYDIEAW
                     RAEFPILKEAVHLANCSQSPQCDRTRAAAEAYLDSWGHMGMDWDRWVQEVNLAKAEFA
                     RIINADPEEVAVGTSVSEITSTIASALDFGGKRRKVVVTDVEFPTVGLVWLANEKYGA
                     KVQFIPAKNGMIELDDYSRTVDKDTLITSFCDV"
     gene            1172..1918
                     /locus_tag="P5313_06025"
     CDS             1172..1918
                     /locus_tag="P5313_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79956.1"
                     /translation="MSFTVSRFFPSEITLSITSGSRFVLDASHATRRRSGTMTPSQRA
                     RAEELYPRVSFERGAVGRTFRDILDGIHEDRAAREGFPLVLITDEKKEYQHALFNNPL
                     FRNQDEVHRVAHLRVHSELPRTFSNPLFASNYLDREIRKDLANLHRETVCFSRNVANG
                     MSRLLCYLVHHNYRKRYRIGGRADQDRVHGEAAGIPRRRIEQALTEMFSRRAFLSRTF
                     LTESQRRIWVKGYPTPLKLEVDYLPSYAAA"
     gene            2011..3006
                     /locus_tag="P5313_06030"
     CDS             2011..3006
                     /locus_tag="P5313_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HRY79957.1"
                     /translation="MSWTPYESTQRSPARNLAWEDALLESRERGSSVFLVYRDDPCVV
                     IGRNQNPWREALTGAGFSVFRRRSGGGTVYHDGGNLNWSFLVDRDAWVVDDALDFVGT
                     ALRRLGLETVRDPRGALFVEGGKVCGTARRYFGPSVLIHGTLLVCSDLDALRASLAGI
                     PSAGDRAVASVPSRVRNLADVLPGLTVRDVRDVLFAELAARYGACEPEDPGRVLPVDS
                     WADREREHGSWDWVFGSTMPFTVALGPTRAQGDGTLYLRVRGGRADRVQSCPDCASVA
                     DEQGAAAEDPSFGPLPEAWAGRPFDAALLGELRAWAAEATEAGIPRDASVVPETG"
     gene            3056..3502
                     /locus_tag="P5313_06035"
     CDS             3056..3502
                     /locus_tag="P5313_06035"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRY79958.1"
                     /translation="MEFKEIVSVLHSLLEESKVGVLTTIDGRGYPHSRWMTPALLPRF
                     PGRLFAVTGSGFGKVENIRANPKVEWAFQSRTLDRVVSLKGNAFVVDEPELKVEVLEA
                     IGPNLQIFWRINPKPGDLVVLETEIEEATLFLPLKRESHRVEASHG"
     gene            3495..4544
                     /gene="pdhA"
                     /locus_tag="P5313_06040"
     CDS             3495..4544
                     /gene="pdhA"
                     /locus_tag="P5313_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRY79959.1"
                     /translation="MADKKTLGGHDKKFLMGLLESMHRIRAFEEKAGQMYGLRKIGGF
                     CHLYSGQEAVAVGVISALDLSTDYVLTGYRDHGHALACGMDPKAAMAELYGKAAGCSK
                     GKGGSMHFFDAGKHMLGGNGIVGAQIPVAAGVAFAQAYRGRLSGSQPTGATVCFFGDG
                     AIHQGAFHEAFNLAGIWKLPLILVVENNQWGMGTSWKKVSSNEDFSAMAGAYAAHGEA
                     VDGMDVLAVHDAAVRAVKRAKAAGEPALLEARTYRYKGHSMSDPQKYRTREEVETYRA
                     RDPILLLEGRMEEAGLLSAREKEALRAEAEAIAEEAAAFAEASPEPAADELYTDVYAD
                     RESGETAPSEASWQS"
     gene            4532..5515
                     /locus_tag="P5313_06045"
     CDS             4532..5515
                     /locus_tag="P5313_06045"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRY79960.1"
                     /translation="MAVLTYREALNRALDEELARDDRVLLMGEEVGEYDGAYKVSKGL
                     LAKYGPNRVLDTPITELGFTGLGVGAAMAGLRPVVEWMTHNFALLALDQVVNEAAKMR
                     YMSGGQFSVPIVFRGPNGPAEYLASQHSQALESYWAHVPGLKVVAPATPADAYGLLKS
                     AIRDDDPVVMLEAEMMYGLKGEVPDGEFLVPIGKAEVRRPGSDVTLITYSKPLWTVLE
                     AAEELARQGFSAEVLDLRTLRPLDEEAIYASVRKTNRCVVVTESWPQASVASHVAWLV
                     SRNCFDRLDAPVELVSGEDVPMPYNHALELAAQPNVPKVVAAARRVLYAEG"
     gene            5517..6878
                     /locus_tag="P5313_06050"
     CDS             5517..6878
                     /locus_tag="P5313_06050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRY79961.1"
                     /translation="MAEKLYMIALSPTMTEGTLARWKVSEGQAFKAGSVLCEVETDKA
                     SMDYEAPKDGVLLKVLLAEGGRAAVGDVIGIIGKAGESPDALLAQLSPGAGGAGSKAG
                     GTEAAAAAPTVAASAASASPVPGTAGPGLSRPVEPPVAAHAGPAPSSPLARVLAAELG
                     VDIRTLRGTGPGGRVVKRDVEAAAAGGSGGPAPGRAGPVPAGARPGSGPSSSAVPRGP
                     TGEQTVRMEPVAGKRAVIARRLSESFFSAPHYYLKRTVDAGRLLALREALADPEGRKP
                     SINALLAKLAAAALAGHPAVNAGWKDEGAGKASVEYRSRVDIGLAVALPDGLITPVVR
                     DCGSKGISRIDAEMSDLVAKAKGSGLSPEEYTGATFTITNLGGFGVEEFTAIINPPGS
                     AILAVGAALDEPVALPDGTVAVRKRMRLTLGCDHRVIDGAVGAAFLRDLAGMIEEPGR
                     ALA"
     gene            7232..8530
                     /locus_tag="P5313_06055"
     CDS             7232..8530
                     /locus_tag="P5313_06055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY79962.1"
                     /translation="MNDSDFRSLGLAESLLSTLAEEGYASPTPIQQKTIPSLLAGKDL
                     LGVAQTGTGKTAAFALPILQRLAAFPARPEPRAARVLVVAPTRELAAQIDESFGTYGR
                     RFRFSRACVFGGVGKNPQIKALSRGVDVLTATPGRLLDLHGEGYIRLDRVETVVLDEA
                     DRMLDMGFIHDVRRIVALLPKKRQTLLFSATMPRDIAVLASSILDNPTRVDVEPEKPT
                     VDKVDQHILHVRKENKKDLLAHLLRKADVRRAVVFTRTKHGANRLSAALGAEGISSEA
                     IHANKSQNARIRALDGFKRGEIRVLVATDLAARGIDVDGITHVFNYELPNEPETYVHR
                     IGRTARAGASGSALSFCDAEERPLLRQIERLLGRPVPVEADHPYVSAVSTVSAESPAA
                     PAGMRKVMPSRTMRSRNFRSSSSDRGTDNRNPWKPRQPMR"
     gene            complement(8542..9891)
                     /locus_tag="P5313_06060"
                     /pseudo
     CDS             complement(8542..9891)
                     /locus_tag="P5313_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
     gene            complement(10013..10095)
                     /locus_tag="P5313_06065"
     tRNA            complement(10013..10095)
                     /locus_tag="P5313_06065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10060..10062),aa:Leu,seq:taa)
     gene            complement(10195..11664)
                     /locus_tag="P5313_06070"
     CDS             complement(10195..11664)
                     /locus_tag="P5313_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79963.1"
                     /translation="MDETKIFGNRLPGRALKSAERTFRRYARRFAFSPDDSPVLGAVP
                     MGPAYDLFGVRKLEERSPDGGPYEEIDREKGIVLGTIRMGFGHYRIGLALASAARSMG
                     LTPYWMDLMSFPDTAASRTIDYLEDLYNLGSRVSQRFKWFDKYVWEKITSDMAKRLSY
                     TARDRALARLFAPLLGGVPRDIPFLSTHPWTGHAAVKAGMGDVVTLIPDNYPLAFHLV
                     EGSVHVVQTPSAYMGYRTLRDMGGQEELMYALPRDDIRYVGHYVDHEIVSAVEEDCSY
                     RLRRLRDKETRRFLLTMGGAGAQARRFADIARACKGAIEEGKLALLVNMGDHAGRWAE
                     LRSSLDADGIRYTLHSDWTETQAFAQEIRTGSVSGVHVFLHEAFFAAVYTTNLLMRAS
                     DIMITKPSELAFYPVPKLFIQRVGRHEAWGAIRGSEIGDGTLETASDASLHQALRLLV
                     EENDLLEMYIGNILRNARAGIYDGAYGAVHLALERRNRT"
     gene            complement(11813..12133)
                     /locus_tag="P5313_06075"
     CDS             complement(11813..12133)
                     /locus_tag="P5313_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRY79964.1"
                     /translation="MDSLRILASIAVMVAATAATRALPFLVFRDRKVPGFVPFLAGYG
                     PPAILTILTLYCFKDVDWTAAPHGAPELAAAALTAVLHLWRRNTLLSIVGGTALYMVL
                     IRMV"
     gene            complement(12126..12845)
                     /locus_tag="P5313_06080"
     CDS             complement(12126..12845)
                     /locus_tag="P5313_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRY79965.1"
                     /translation="MESTDRGGAAGAPRAALRAALPATFPVFLGYTTIGAAFGLLLAS
                     SGLPLWAAPAMSLFVYAGAAQFMGVGIIASGAGLAELAAATFLLNLRHMVYGLSFLEL
                     YSRAGARKPYLVFTLTDETYALLSTVSVPPGVDPDRFRFWVSALNQSYWVLGTLAGTL
                     AGGLAAFDTRGLEFSLTALFTVLLIEQTRKVRSPLPYLASGACAAAAYLALGPSRMLL
                     PAIGAAGVLLFALRKRLEARG"
     gene            13061..13225
                     /locus_tag="P5313_06085"
     CDS             13061..13225
                     /locus_tag="P5313_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79966.1"
                     /translation="MAGEMAHSLRGIALDRRGRLASAARVRAAFAAQNPTLSSLYILT
                     IIVLLLILGM"
     gene            13594..14193
                     /gene="ilvN"
                     /locus_tag="P5313_06090"
     CDS             13594..14193
                     /gene="ilvN"
                     /locus_tag="P5313_06090"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRY79967.1"
                     /translation="MVPLREPVGMAVSPEIPTAERFVVSALVQNHPGVLSRISGLFSR
                     RGFNIESLSVGETLDPSVSRMTVSAIGDEAVLEQLARQLEKLEDVLSILRLEPGAGVF
                     RELVLVKVKATPETRGDIVNIARIFRARIVDVSRREMILEITGERPKIDGLLDLLGPY
                     GIPEIARTGLTALQRGASTLSRPEPAESAPYGAVPTTTL"
     gene            14204..15202
                     /gene="ilvC"
                     /locus_tag="P5313_06095"
     CDS             14204..15202
                     /gene="ilvC"
                     /locus_tag="P5313_06095"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRY79968.1"
                     /translation="MATMYYDKDCDKKALAGKTIAVIGFGSQGHAHALNLRDSGYPVV
                     VGLYEGSKSWKEAEAQGFKVMTAADASASAEFIMILINDEKQARLYSESVKPGLKSGK
                     TIAFAHGFNIHFQQIVPPPEVDVVMIAPKGPGHTVRSQYQEGKGVPCLVAVQQDASGT
                     AKAQALAYAAGIGGARAGVLETTFQEETETDLFGEQCVLCGGVTSLMKAGFDTLVEAG
                     YQPESAYFECIHEMKLIIDLVVQKGISFMRYSISDTAEYGDYTVGPRIITEETRKEMK
                     RVLAAIRNGQFAREWILENQANRPAFNRMRAIEAAHPSEKVGAELRAMMSWTRDKR"
     gene            15212..16804
                     /locus_tag="P5313_06100"
     CDS             15212..16804
                     /locus_tag="P5313_06100"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRY79969.1"
                     /translation="MAREIAIFDTTLRDGEQAPGCSMNLGEKLEVARQLEKLGVDVIE
                     AGFPIASPGDLASVREIARQVRLCAVAGLARAVPGDIDAAWEALRPAERPRIHTFIAT
                     SPVHMEYKLRMKPDEVVEQAVAMVRYARKLCPDVEFSAEDASRSDPDFLCRIFQAAIE
                     AGASTVNLPDTVGYALPRQFGDLVRRVKAGVPDMGKAVFSVHCHDDLGLAVANSLAAV
                     EAGADQVECTVNGIGERAGNASLEEIAMILRTRADSLDARCRIDTTRIYAASRLVVQV
                     TGCRVQPNKAVVGENAFAHESGIHQHGVLARADTYEIMTPESVGLPRNKMVLGKHSGR
                     HAFDDRLAELGFHLGPARTAELFKAFKALADKKKTVSDRDIEALARGWAPDRKAAWKL
                     ERFIINSGNSITSTSAVSLASRDGRKEERVATGDGPIDASFKAIDKIVGRDPVLEDFS
                     LDSVTGGKDAQGEAHVRVRLGDRAYNGHGVSTDVIEASVRAYIDAINAMAEDLDAEGG
                     PGPSSAGAEEDADGQASARNAS"
     gene            16773..18425
                     /gene="cimA"
                     /locus_tag="P5313_06105"
     CDS             16773..18425
                     /gene="cimA"
                     /locus_tag="P5313_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRY79970.1"
                     /translation="MDRLPRVTLLDSTLRDGAQGEGVSFSVTDKISVVRALDALGVDI
                     IEAGNPGSNPKDAEFFREARGLRLARSTLAAFGSTRRRDTRPESDAQLASLLAADTGI
                     VVLFGKSWTFHVRAVLGVSLEENLAMIGDSVAFLRSAGRRVIFDAEHFFDGFAADPDY
                     ALACLRSARDAGAECMVLCDTNGGSLPSRVAEFTRRAAVVGAPLGIHAHNDSGLGVAN
                     SLTAVEAGAVHVQGTLAGFGERCGNANLSTVAADLQLKMGRDCLGPGALAGLAGAVRR
                     VTEIANLTPDEGMPYVGRRAFAHKAGMHVDAVRKDPASFEHVPPGAVGNERRFLASEV
                     GGRSVLMDRFRRILPDAAKDSPEVVRAAEALKKMEGLGYQYEGAEASLEILLRKAAGS
                     YVPSFKLRRYRTTGEHPAEDPSACAHAMVQLEVEGRREISAAEGCGPVHALDLALRKA
                     LVGFYPSLAGMRLADYKVRVLPGTGATASVVRVLIDSSDSRDVWTTVGVSADILEASW
                     IALVDSYEYKLSKDTQSETRRETGDEHADDDDAEDLGRACGA"
     gene            18376..19632
                     /gene="leuC"
                     /locus_tag="P5313_06110"
                     /pseudo
     CDS             18376..19632
                     /gene="leuC"
                     /locus_tag="P5313_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
     gene            19746..20273
                     /locus_tag="P5313_06115"
     CDS             19746..20273
                     /locus_tag="P5313_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRY79971.1"
                     /translation="MTAAGRAFKYGDNVDTDAIIPARYLNTSDPAELAAHCMEDLDPS
                     FAGAVRPGDVLVGGRNFGCGSSREHAPVAIKAAGVSCVIAASFARIFYRNAVNIGLPI
                     LESPEAAAGIEAGDRLSLDFGSGTISDGTKNSSWRSAPFPPFMRRIMEAGGLTEYVRA
                     RLTEAGDSGAEGGGS"
     gene            20270..>20919
                     /locus_tag="P5313_06120"
     CDS             20270..>20919
                     /locus_tag="P5313_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRY79972.1"
                     /translation="MKNAITLVPGDGIGLEVTAQAVRVLEAVGAQFGVSFRFETVLLG
                     GAAIDEKGVPLPAETLAACKAADAVLLGAVGGPRWDDLSGARRPEAGLLGLRAGLGVY
                     ANLRPARLFPELRSACPLKDEIAGKGFDLLIVRELTGGMYFGDRGRSPDGEVAWDTER
                     YSRKEITRLLTMAFRAARSRSGRLCVVDKANVLESSRLWRETSRALSPAWPDVEVS"
BASE COUNT         3396 a         6905 c         7292 g         3326 t
ORIGIN      
        1 gacgtcgcag aaggaggtga tcagggtgtc cttatccacc gtccgggagt agtcgtccag
       61 ctcgatcatc ccgttcttgg cggggatgaa ctggaccttg gccccgtact tctcgttggc
      121 cagccacacc aggcccaccg tcgggaactc cacgtccgtg acgaccacct tccgccgctt
      181 gcccccgaag tccagggccg aggcgatggt gctggtgatc tcggacaccg aagtcccgac
      241 cgcgacctcc tcgggatccg cgttgatgat ccgcgcgaac tccgccttgg ccaggttgac
      301 ctcctggacc cagcggtccc agtccatgcc catgtggccc cagctgtcca ggtaggcctc
      361 cgcggcggcc cgggtccggt cgcattgggg ggactgggag cagttggcca ggtggacggc
      421 ctccttgagg atcgggaact cggctctcca ggcttcgatg tcgtagccct cggggccgga
      481 gccgccggac ccggctctcg ggacacgggc gggggcgctt gaggggcgga aggactcggg
      541 cacgaaagaa gccgcgggcg aggcatggga tccggcgccg cttcgaggcg aagccgccga
      601 agtgtcggat cccggtgcga cgatgctgat cccgtagcgc tcgcagtttt cccgggctat
      661 gcccgtaagc ctgtcgttcg ggcccaggac gagccggctg ttccccgact tggccagtgc
      721 ctctatatcc tcacccgtga cgtaattgat cattcggcgc gctccctggg aattggtagt
      781 gccgagtata gaacggatcc gagaatgacg caagcaaaaa aggcaaggaa cctacataat
      841 gtatagctaa atcaaataaa tgttacatag tgaatgaaat cccttctgtt ttcaccatat
      901 tggtatcatg ttagcagcaa cctcattcaa tccgtggaag tggttttcga agtgttttcg
      961 agtaggaaat acaaacctca ggttaggaga ttccgcatat atcacgaagt attatatagt
     1021 tgggttcgct tatctcatgg ctatctttat gatgtctaat tttacctgtt tgaggttgga
     1081 ttcctcatta gatatgatta ataacatata ttgagtaaaa cttcaatttg ctattgctta
     1141 gaaggcgact gtttggcagt aataattcct cttgtctttc acggtctccc gattctttcc
     1201 tagtgagatc accctgtcga tcacctcggg ctcccggttc gtcctggacg cgagccacgc
     1261 cacccgcaga cgctccggca ccatgacgcc ctcccagaga gcccgggccg aggagctcta
     1321 cccccgggta tctttcgagc ggggagcggt cggcaggacc ttccgggaca ttctcgacgg
     1381 gatacatgag gacagggctg cccgggaagg attccctctg gtgctaatca ccgatgagaa
     1441 aaaggaatac cagcacgccc ttttcaacaa tccactcttc cgaaaccagg atgaggtcca
     1501 cagggtcgcc cacctccggg tccattccga gctcccgagg actttctcta atccactgtt
     1561 cgcatcgaac tacctggatc gggagatccg gaaggacctg gccaacctcc atcgtgagac
     1621 ggtctgcttt agccgcaatg tggccaacgg gatgtcccgg ctcctgtgct accttgtgca
     1681 tcacaactat aggaaacggt acaggatcgg agggagggct gatcaggata gagtccacgg
     1741 ggaggcggcg gggatacccc gaagaaggat cgagcaagcc ctcacggaga tgttcagccg
     1801 gagggcgttc ctgagccgga ctttcctgac ggaatcccaa cggaggatct gggtgaaggg
     1861 gtatccgaca cctctcaagc tggaggtcga ctacctccct tcctatgcgg cggcttaggt
     1921 catgcctgga gatccagccc ttggcttcac aaggttcgta acactatacg gattttgcgt
     1981 ttccttcctg gttccagtat cttggaagac gtgagctgga caccctacga gtctacacag
     2041 cgctctcccg caaggaacct cgcctgggag gacgccctcc tggaatcccg ggaacggggg
     2101 tcctccgtgt tcctggtcta ccgtgacgat ccctgcgtgg tgatcggccg gaaccagaac
     2161 ccctggaggg aggcgttgac gggggcgggt ttttcggtgt tccggcgccg gagcggaggc
     2221 gggacggtct accacgacgg ggggaacctg aactggtcct tcctggtgga ccgggacgcc
     2281 tgggtcgtgg acgatgccct ggatttcgtc gggacggccc tccgccgcct ggggctcgag
     2341 acggtccgcg atccccgggg agccctcttc gtggagggcg gcaaggtctg cgggacggcc
     2401 cggaggtact tcggaccctc ggtgctgatc cacgggaccc tcctggtatg ctcggacctg
     2461 gacgccctcc gggcctccct ggccgggatt ccctcggccg gggaccgggc ggtggcatcc
     2521 gtgccctccc gggtgcgcaa cctggcggat gtcctgccgg gcctgacggt gcgggacgtc
     2581 cgggacgtcc tcttcgcgga gctggcagcc cggtacgggg catgcgaacc ggaagacccg
     2641 ggtcgagtcc tgcccgtgga ctcctgggcg gaccgggagc gggagcacgg atcctgggat
     2701 tgggtcttcg gctccacgat gcctttcacc gtggctctcg ggccgactcg agcgcagggc
     2761 gacgggacgc tttaccttcg ggtgcggggg gggagggcgg accgcgtcca gtcctgcccg
     2821 gattgcgcct ccgtagccga cgaacaaggg gccgccgcgg aggacccgag ctttggtccg
     2881 cttccggaag cctgggcggg caggcccttc gacgcggccc tgctcgggga gctccgggcc
     2941 tgggcggccg aagcgaccga ggccgggatt ccccgggacg cctcggttgt gccggaaacc
     3001 ggatgagggg cggagattcg acgacccgga ttcgtactcg gagaggagat ttactatgga
     3061 gttcaaggaa atcgtatccg tcctgcattc gctcctggag gaatccaagg tgggggtgct
     3121 caccacgatc gacggccggg gctaccccca ttcccggtgg atgacccccg ccctccttcc
     3181 ccggtttccg ggaaggctct tcgccgtgac gggctccggg ttcgggaagg tcgagaatat
     3241 ccgggcgaat ccgaaggtgg agtgggcctt ccagagccgc accctggacc gcgtcgtctc
     3301 cctgaagggg aacgccttcg tggtggacga gccggagctc aaggtcgagg tcctggaggc
     3361 gatcggtccg aacctccaga tcttctggag gatcaacccc aagcccgggg atctggtggt
     3421 cctggagacg gagatcgagg aggcgaccct cttcctgccg ctcaagcggg agtcccaccg
     3481 ggtggaggcg agccatggct gacaagaaaa ccctcggcgg gcacgacaag aaattcctga
     3541 tgggcctgct cgagtcgatg caccgcatac gggccttcga ggagaaggcg gggcagatgt
     3601 acggactccg gaagatcggg ggcttctgcc acctctacag cggccaggag gccgtggcgg
     3661 tcggggtgat ctcggccctg gacctctcca cggattacgt tctgacgggc tatcgggacc
     3721 acggccacgc cctggcctgc ggcatggacc ccaaggccgc catggcggag ctctatggga
     3781 aagccgcggg ctgctccaag gggaagggcg ggtccatgca cttcttcgac gccggaaagc
     3841 acatgctcgg aggcaatggc atcgtggggg cccagatccc cgtggccgcg ggcgtggcct
     3901 tcgcccaggc ctaccggggc cgtctgtccg gctcccagcc tacgggcgcc acggtctgct
     3961 tcttcgggga cggggctatc caccaggggg ccttccacga ggccttcaac ctggcgggga
     4021 tctggaagct ccccctgatc ctggtggtgg agaacaacca gtggggtatg ggcaccagct
     4081 ggaagaaggt ctcctccaac gaggacttct ccgccatggc cggggcctac gcggcccatg
     4141 gcgaggcggt ggacggcatg gacgtgctgg cggtccacga cgcggccgtg cgggccgtga
     4201 agcgggccaa agcggccggc gagccggccc tcctggaggc ccggacctac cggtacaagg
     4261 gccactccat gagcgatccc cagaagtacc gcacccggga ggaggtcgaa acctaccgcg
     4321 cccgggatcc catcctcctc ctggaaggac gcatggagga ggcggggctc ctgtccgccc
     4381 gggagaagga ggccctccgg gccgaggcgg aggcgatagc cgaagaggcc gcggctttcg
     4441 cggaggccag ccccgagccg gcggccgacg agctctacac cgacgtctac gcggatcggg
     4501 agtccggcga aacggctccc tcggaggcat catggcagtc ctgacatatc gagaggccct
     4561 gaaccgggcc ctggacgagg agctcgcccg ggacgaccgg gtcctcctca tgggggagga
     4621 ggtcggggag tacgacgggg cctacaaggt ctccaagggc ctcctggcca agtacggtcc
     4681 gaaccgggtc ctggacactc ccatcacgga gctgggcttc acgggcctgg gggtgggggc
     4741 cgccatggcg ggtctgcggc cggtggtgga gtggatgacc cacaacttcg ccctcctggc
     4801 cctggaccag gtggtcaacg aggccgccaa gatgcgctac atgtccggcg gccagttttc
     4861 ggttcccatc gtcttccggg gtcccaacgg cccggcggag tacctggcca gccagcactc
     4921 ccaggccctg gagagctact gggcccacgt cccgggcctc aaggtagtgg ccccggccac
     4981 ccccgcggac gcctacggtc tcttgaagag tgctatccgg gacgacgatc ccgtggtgat
     5041 gctggaggcc gagatgatgt acgggctcaa gggcgaggtc cccgacgggg agttcctggt
     5101 gcccatcggc aaggccgaag tgcggcgccc gggatccgac gtcaccttga tcacctactc
     5161 caagcccctg tggaccgtcc tggaggccgc cgaggagctg gcccggcagg gcttctcggc
     5221 cgaggtcctg gacctccgga ccctccgccc cctggacgag gaggcgatct acgccagcgt
     5281 gcggaagacc aaccgctgcg tggtggtgac cgagtcctgg ccccaggcca gcgtcgcctc
     5341 ccacgtggcc tggctggtat cccggaactg cttcgaccgc ctggacgccc cggtggagct
     5401 ggtctcggga gaggacgtgc ccatgcccta caaccacgcc ctggagctgg cggcccagcc
     5461 gaacgtgccc aaggtcgtgg ccgcggcccg gcgcgtcctg tacgcggagg gatgagatgg
     5521 cggaaaaact ctacatgata gctctgtccc cgaccatgac cgaggggacc ctggcccggt
     5581 ggaaggtgtc cgagggccag gccttcaagg ccggctcggt gctctgcgag gtggagaccg
     5641 acaaagcctc catggactac gaggccccca aggacggggt cctgctcaag gtcctcctgg
     5701 ccgagggcgg ccgggccgcc gtaggggacg tgatcgggat catcggcaag gccggggaga
     5761 gcccggacgc cctgctggcc cagctctccc cgggggcggg cggagcgggc tcgaaagcgg
     5821 ggggtaccga ggcggccgcc gcggctccga ccgtcgccgc ctcggcggca agcgcctccc
     5881 cggtacccgg caccgcgggc ccggggcttt cgcgcccggt agagccgccc gtcgccgcgc
     5941 acgcgggtcc ggccccctca tcgcccctgg cccgggtcct ggccgcggag ctgggggtgg
     6001 acatccggac tctccgaggc acgggccccg gcggccgggt ggtcaagcgg gacgtggagg
     6061 ccgccgcggc gggcggatcc ggcggcccgg ccccgggccg ggcgggtcct gtcccggcgg
     6121 gtgctcgccc cgggtcgggc ccctcgtcgt cggccgttcc acggggaccc acgggagaac
     6181 agacggtccg gatggagccc gtggcgggca agcgggcggt catcgcccgg cgcctctcgg
     6241 agagtttctt ctcggcgccc cactactacc tcaagcggac cgtggacgcc ggccgcctcc
     6301 tggccctgcg ggaggccctg gcggatccgg aggggcgcaa gccctcgatc aacgccctgt
     6361 tggctaagct ggccgccgcg gccctggcgg ggcatcccgc cgtgaacgcg ggctggaagg
     6421 acgagggggc ggggaaggcg tccgtcgaat accggtcccg ggtcgacatc ggcctggccg
     6481 tggccctgcc ggacgggctc atcaccccgg ttgtccggga ctgcgggtcc aaggggatct
     6541 ctcggatcga cgcggagatg tcggacctgg tcgccaaggc caagggctcg gggctgagtc
     6601 ccgaggagta caccggggcc accttcacga tcaccaacct gggaggcttc ggggtggagg
     6661 agttcaccgc catcatcaat ccccccggat cggccatcct ggccgtgggt gcggccctgg
     6721 acgagcccgt agccctgccg gacgggaccg tggcggtgcg gaagcggatg cgtctgaccc
     6781 tgggctgcga ccatcgggtg atcgacgggg ccgtgggcgc ggccttcctg cgggacctgg
     6841 cggggatgat cgaggagccc ggccgggccc tggcgtaggc gggcggcgga gccgcccgga
     6901 agcccggccg gcttccggac ggcccgcacc gccgcggcag cccgtgcggg aacggcgccg
     6961 gtccccccgc tatccacgac cgcccgtttc ctgtatagta ctttcgaacc ggacgtgccg
     7021 ccgatgcgcg cccggagaat gtaaaacatc ggcgccgatc cgatccgcgg gcccgtcggg
     7081 ctgacggaac gggtccggat atggtagccg aatcctgaag agccgcccgc cgcgttcccg
     7141 agagggatcg cgtccgaatt aaccttcgcg attcaccgtt caccatccac accacaagct
     7201 tcgtgacaca taggtcacga caaggatttc catgaacgat tccgatttcc gctccctggg
     7261 gctcgccgag tccctcctct cgaccctggc cgaggaaggc tacgcctctc ccacgcccat
     7321 ccagcaaaag accatcccca gcctcctggc cggaaaggac ctcctgggcg tggcccagac
     7381 gggcaccggc aagaccgcgg ccttcgccct gcccatcctc cagcgcctgg ccgccttccc
     7441 ggcgaggccg gagccccggg ccgcccgggt cctggtcgtg gcccccaccc gggagttggc
     7501 cgcccagatc gacgagagct tcggcaccta cggccggcgg ttccgcttct cccgggcctg
     7561 cgtcttcggg ggggtcggca agaatcccca gatcaaggcc ctctcccggg gggtcgacgt
     7621 cctgaccgcc accccgggcc ggcttctgga cctgcacggc gagggctata tccgcctgga
     7681 ccgggtggag acggtggtcc tggacgaggc ggaccgcatg ctcgacatgg gcttcatcca
     7741 cgacgtgcgg cgcatcgtcg ccctgctccc gaagaagcgg cagaccctcc tcttctccgc
     7801 gaccatgccc cgggacatag ccgtcctggc ctcgtccatc ctggacaacc ctacgcgggt
     7861 ggacgtggag cccgagaagc cgacggtgga caaggtggac cagcatatcc tgcacgtgcg
     7921 caaggaaaac aagaaggacc tgctggccca ccttctccgc aaggcggacg tgcgccgggc
     7981 cgtggtcttc acccggacca agcacggcgc caatcgcctc tccgcggccc tgggtgcgga
     8041 gggaatctcc tccgaggcca tccacgcgaa caagagccag aacgcccgga tccgggccct
     8101 ggacgggttc aagcgcgggg agatccgggt cctggtggcc acggacctgg cggcccgggg
     8161 gatcgacgtg gacgggatca cccatgtctt caactacgag ctgcccaacg agccggagac
     8221 ctacgtgcac cgcatcggcc ggacggcccg cgcgggcgcc tcgggctcgg ccttgtcctt
     8281 ctgcgatgcc gaggagcggc ccctcctgcg ccagatcgag cgcctcctgg gccggccggt
     8341 gccggtcgag gcggaccacc cctacgtgtc ggcggtctcc acggtctccg cggagtcccc
     8401 ggcggccccc gcggggatgc ggaaggtcat gccgtcccgg acgatgcggt cccggaactt
     8461 caggagctcc tcgtcggaca ggggtacgga caacaggaat ccctggaagc cccggcagcc
     8521 catgcggtag aggtgtcgga gcgcgtccgc gcgctccacg ccctccgcga tcaggtccag
     8581 gcccaggtcc cgggctattc cgaccaggga ccggacgatg gatcggtcct ggtggttctg
     8641 gtgcaggttt tccacgaata gacggtcgat cttgatctcg tccaggggga agcggtgcag
     8701 gtggctcagg gaggaatacc ccgtcccgaa gtcgtccacc gagacccgca cgcccatggc
     8761 cgcgaagcgc tggaggatcc gcgtggtggc ctcggcgtcc tcgaaaaggc tcgtctccgt
     8821 gagctccacg gtcagcgccc gttcggggat cttggaggac gagatcagcc ccgccagggt
     8881 ttcttccacc gctccggacc ggaactggcg gggcgagagg ttcacggaca ggccgatgtc
     8941 ggcccggccg gatttgtgcc agctcgacaa cctgcgcacg cactggtaga ccagccactt
     9001 gcccaggggc tcgatgagcc ccgtctcctc cgccaggggt atgaaggtcg cgggcgctat
     9061 gggaccccgg gtcgggtggt tccaccggat cagggcctcc gcctcgatcc tctccacctg
     9121 aagcaccctg ccttcgatcc cggacacgat cagcttgggc tggaagacta gggagaactg
     9181 ctcccccagg tggcggatgg cgggggcctc gatggcccgg atgatagagt tctggagctc
     9241 cacccgctcc accacggtct gcaacaggtt ctgacggaac agtacgaagc tcttcttccg
     9301 ctcctgggcg gagcccgcgg caacatccgc gtagtgcagg aggttggacc gggtccggcc
     9361 gtgctcgggg aagtacgccg ccccggaggt gaccggcagg gagaccgtga agcccgcgaa
     9421 gacgtggggt tcacagaggg aggacagaag tcgacgtagg accttcttga gctcctgccc
     9481 cttggctccc cggagcacca ggacgaactc gtcctcccgg gtgtggtaga cccggtcctc
     9541 gggtcccagg accgcgttca cccggcaacc cgtctggtac aggatccatt cgctgatgct
     9601 ggacctcagg gtcttgcgga ccatggagta ggtctgtccc agctggacga tgaggaccgt
     9661 gaacccccgg gtgtccggtt tcgcgttctc ggattcgatc agatcgtcca ggtcgatctc
     9721 cagccggaag tgggtgggca ggcccgactg ggcctggaag gagatcttcc gctcgaggtg
     9781 ggctactttg gtctcgagga cttgtacctg ggcgcggagc cgggtgtttt cctccgagat
     9841 ggacgaaaca agagttgtct tgccttcctg gctcttcgtt gcagacgtca tccggcgctt
     9901 ccatccttgt aggcgcttgt cccggccccg gggacagagt tgttcagttt cttatcggca
     9961 gatatatatc caaaccttga gtgtttccgc ccgcggctcg gcggatttcg gttgccggga
    10021 accagacttg aactggcacg gcctcgcggc caggggattt taagtccccg gtgtctacca
    10081 ttccaccatc ccggcgcgtc tccggggtgt ccaataggtc ggttgcttat tggacagccc
    10141 ctatcatcgg attcgcatgg tacccgtgtc cggtccggcg gtcaagcttg gtgttcaggt
    10201 acgattccgg cgctccagtg ccaggtggac ggccccgtag gccccgtcgt agatccccgc
    10261 ccgggcgttc ctcaggatgt tcccgatgta catctccaat aggtcgttct cctccaccag
    10321 gaggcgcagg gcctggtgga ggcttgcgtc cgaggcggtc tccagggtcc cgtccccgat
    10381 ctcggagccc cggatggcgc cccaggcctc gtgccggccc acccgctgga tgaagagctt
    10441 gggaaccggg tagaaggcga gctcgctggg cttggtgatc atgatgtccg aggcccgcat
    10501 gagcaggttc gtggtgtata ccgccgcgaa gaaggcctcg tgcaggaaga cgtggacccc
    10561 cgagacggag cccgtccgga tctcctgggc gaaggcctgg gtttccgtcc agtccgagtg
    10621 cagggtgtag cggatgccgt cggcgtccag agaggagcgg agctcggccc agcggcccgc
    10681 gtgatccccc atgttcacca ggagggccaa cttcccctcc tcgatagccc ccttgcaggc
    10741 ccgggcgatg tccgcgaagc gccgcgcctg ggcaccggcg ccgcccatgg tcaggaggaa
    10801 gcgccgggtc tccttgtccc gcaggcggcg caggcggtag gagcagtcct cctccaccgc
    10861 ggatacgatc tcgtggtcca cgtagtgccc cacgtagcgg atgtcgtccc ggggaagggc
    10921 atacatcaac tcctcctggc cgcccatgtc ccggagggtt cgatacccca tgtaggccga
    10981 gggggtctgc actacgtgta cgctcccctc gaccagatgg aaggccaggg gatagttgtc
    11041 cgggatgagg gtgaccacgt cccccatccc cgccttcacc gcggcgtggc cggtccaggg
    11101 gtgtgtggac aggaagggaa tgtcccgggg gacgccgccc agcaggggcg cgaagagccg
    11161 ggccagggcc cggtcccggg cggtatagga aagccgtttg gccatgtcgg aggtgatctt
    11221 ctcccacacg tacttgtcga accacttgaa gcgctgggat acccgggagc ctaggttgta
    11281 gaggtcctcc aggtagtcga tggtccggct ggcggcggtg tccgggaagg acatcaggtc
    11341 catccagtag ggggtgagcc ccatggaccg ggccgcggag gccagggcca gcccgatccg
    11401 gtaatgcccg aagcccatgc ggatcgtgcc caggacgatg cccttctccc ggtcgatctc
    11461 ctcgtaggga cccccgtccg ggctccgctc ctccagcttg cggaccccga agaggtcgta
    11521 ggcggggccc atgggcacgg ctcccagtac gggcgagtcg tccggggaga aggcaaagcg
    11581 gcgggcgtag cggcggaagg tccgttcggc gctcttcagg gctcgtccgg gcagccggtt
    11641 tccaaaaatt ttcgtctcgt ccacggtttt tcctctcccg gccccggcgc gggcgcccgg
    11701 aagccgtcgc ttcgagtata gccgatgccg ggcggggcgc aaggtgggga tgcgggccgc
    11761 gcccgatacc agcccgggcg tcggaggctc gagaaaggct gcggccggag cttcacacca
    11821 tccggatgag gaccatgtag agggcggttc ccccgacgat ggacaggagg gtgttcctcc
    11881 tccaaaggtg caggaccgcg gtcagggcgg ccgccgccaa ctccggggct ccgtgggggg
    11941 cggcggtcca gtcgacgtcc ttgaagcagt acagggtcag gatcgtcagg atcgccgggg
    12001 gtccgtagcc cgccaggaag gggacgaatc ccgggacctt ccggtcccgg aagaccagga
    12061 agggcaaggc ccgggtggcc gccgtggccg cgaccatcac cgcgatggag gccaggatcc
    12121 ggaggctatc cacgggcttc cagccttttc ctcagggcga agaggaggac ccccgcggcc
    12181 ccgatggccg gaaggagcat ccgggacgga cccagggcca ggtaggcggc cgcggcgcag
    12241 gcccccgagg ccaggtaggg cagggggctg cggaccttcc gggtctgctc gatcaggagg
    12301 accgtgaaca gggcggtcag ggagaactcc aggccccggg tgtcgaaggc ggcgagtccc
    12361 cccgccaggg tcccggccag ggtgcccagg acccagtagg actggttcag ggcggacacc
    12421 cagaaccgga accggtccgg gtcgaccccc gggggaacgg agaccgtgga gagcagggcg
    12481 taggtctcgt cggtcagtgt gaagaccagg tagggtttcc gcgcacccgc ccgggagtag
    12541 agctccagga acgataggcc gtagaccatg tggcgcaggt tgagcaggaa ggtggccgcg
    12601 gcgagctccg caagacccgc gcccgaggcg atgatcccca ctcccatgaa ctgcgcggcc
    12661 ccggcgtaca cgaacaggct catcgccgga gcggcccaga ggggaagtcc cgagctggcc
    12721 aggagcaggc cgaaggccgc cccgatggtc gtgtagccca ggaagaccgg gaaggtcgcg
    12781 ggcagggccg cccgaagggc cgctctcggg gcaccggcgg ccccgcctcg atccgtactc
    12841 tccatgggaa gggactctat accggtccgg cgggcggccg caagggccgg gatggaccga
    12901 ctcgcggcgg ctctcgggat ttaatttctt ggatattgta gagggaatcg agcaaaagtc
    12961 gaatagaatc ctacattttt actgaaatat ttcaacaatt cgaacgaatt ctatgcatag
    13021 cagcttgaca ctcgtttttt cggcgtgcta gggtccgccc atggccggtg aaatggcgca
    13081 ctccctccgg ggaatcgccc tcgaccgccg cgggcgtctc gcctccgcgg cccgcgtccg
    13141 ggcagccttc gccgcacaga atcccaccct atcctccctc tacatcctca ccattatcgt
    13201 cctcctcctt atcctgggca tgtagggctc cttcgctgga tgacggccgg gcggagagag
    13261 cccgaccgta ctaggccgtc cctggtggta cgaaccttgt gaaaggtccg cggcgccgga
    13321 cggccttcct ttttgccgtc cttcggcggg accgaaacct ttcacaagct tcgtgacact
    13381 caccccggat cgcccggaac catccggacc gggccgcgcc ctaggaggag ccgtcatgga
    13441 cgcgcgattg tatgcccgaa cgaatcgaac gaactgcccc gcgggacacc ttcggtcccg
    13501 cgaacacggt gatggagctt gccccaggca cgggaccgca ccttcgagat tcgaggtctc
    13561 ccgaaagtcc cgcagggagc cggtcctcga cgtatggtcc ctctgcgaga gcctgtaggc
    13621 atggccgtgt cgccggaaat cccgaccgcg gaacgcttcg tggtgtccgc cctggtccag
    13681 aaccatcccg gggtcctgtc ccggatctcc gggctcttca gccggcgggg attcaacatc
    13741 gagagcctct cggtcggcga gaccctggat ccctcggtct cccggatgac ggtgtccgcc
    13801 atcggggacg aggccgtact ggaacagctg gcccgtcagc tggagaagct ggaggacgtg
    13861 ctctcgatcc tgcgcctcga gcccggggcc ggggttttcc gggaactggt cctggtcaag
    13921 gtgaaggcca cgccggagac acggggagac atcgtgaaca tcgcccggat cttccgggcc
    13981 cgcatcgtgg acgtgtcccg ccgggagatg atcctggaga tcaccgggga acgtcccaag
    14041 atcgacgggc ttctggacct gctgggaccc tacgggatcc cggagatcgc ccgcaccggg
    14101 ctcacggccc tccagcgcgg cgcatcgacc ctcagccggc ccgagccggc ggaatcggcc
    14161 ccgtacggag ccgtaccgac gacgaccctc taaggagaac gacatggcta ccatgtatta
    14221 cgacaaggat tgcgacaaga aggccctggc gggcaagacc atcgcggtga tcggcttcgg
    14281 cagccagggc cacgcccacg ctctcaatct ccgggattcg ggataccccg tcgtggtggg
    14341 cctgtacgag ggatcgaaga gctggaagga agccgaagcc caggggttca aggtcatgac
    14401 cgcggccgac gcctccgcgt cggcggaatt catcatgatc ctgatcaacg acgagaagca
    14461 ggcccggctc tactcggaga gcgtgaagcc cggcctcaag tccggcaaga ccatcgcctt
    14521 cgcccacggc ttcaacatcc acttccagca gatcgtcccc cccccggaag tggacgtggt
    14581 catgatcgcg cccaaggggc cggggcacac ggtccgatcc cagtaccagg aaggcaaggg
    14641 agtcccctgt ctggtggcgg tccagcagga cgcctccggt accgccaagg cccaggcttt
    14701 agcctacgcg gcggggatcg gcggggcccg ggcgggggtc ctggagacca ccttccagga
    14761 ggagacggag acggacctat tcggggagca gtgcgtcctc tgcggcgggg taacctccct
    14821 catgaaggcc ggcttcgaca ccctcgtgga ggccggctac cagcccgaga gcgcctactt
    14881 cgagtgcatc cacgagatga agctcatcat cgacctggtg gtccagaagg ggatctcctt
    14941 catgcggtat tccatctccg acaccgcgga gtacggggac tacacggtcg gcccccggat
    15001 catcaccgag gagacccgca aggagatgaa gcgcgtcctc gcggcgatcc ggaacgggca
    15061 gttcgcccgg gagtggatcc tggagaacca ggccaaccgc ccggccttca accgcatgcg
    15121 ggccatcgag gccgcccatc cctccgagaa ggtcggggcg gaactgaggg ccatgatgag
    15181 ctggacccgg gacaagaggt aggagagagc catggcacgc gaaatcgcga tcttcgacac
    15241 gaccctgcgg gacggcgagc aggctccggg ctgcagcatg aacctgggcg aaaaactcga
    15301 ggtggcccgg cagctcgaga agctcggcgt ggacgtcatc gaggcgggct ttccgatcgc
    15361 aagccccggg gacctggcct cggtgcgcga gatcgcgcgc caggtgcgcc tctgcgccgt
    15421 ggcgggcctt gcccgggcgg tgcccgggga tatcgacgcc gcctgggagg ccctgaggcc
    15481 cgcggagcgc ccccggatcc acaccttcat cgctacctcg ccggtgcaca tggagtacaa
    15541 gctccgcatg aagcccgacg aggtggtgga acaggccgtc gccatggtcc gctatgcccg
    15601 gaagctctgc ccggacgtgg agttctccgc cgaggacgcc tcccgttcgg acccggactt
    15661 cctgtgccgg atcttccagg ccgccatcga ggcgggggct tccacggtca acctgccgga
    15721 cacggtgggc tacgccctgc cccgtcagtt cggggacctg gtgcggaggg tcaaggccgg
    15781 ggtcccggac atgggcaagg ccgtcttctc ggtgcactgc cacgacgacc tgggcctggc
    15841 ggtggccaac tccctggctg ccgtggaggc cggggcggat caggtggagt gcacggtcaa
    15901 cgggatcggg gagagggcgg ggaacgcatc cctggaggag atcgccatga tccttcggac
    15961 ccgggccgac tccctggacg cccggtgccg gatcgacacc acccggatct acgccgccag
    16021 ccgcctggtc gtccaggtca cgggctgccg ggtccagccg aacaaggccg tggtcgggga
    16081 gaacgccttc gcccacgagt cggggatcca ccagcacggg gtactggccc gcgcggacac
    16141 ctacgagatc atgacccccg agtccgtggg tctgccccgg aacaagatgg tcctgggcaa
    16201 gcactcggga cgccacgcct tcgacgatcg attggccgag ctgggcttcc atctcggtcc
    16261 ggcccggacc gcggagctct tcaaggcctt caaggccctg gcggacaaga agaagaccgt
    16321 gagcgaccgg gacatcgagg ccctggctcg gggctgggct ccggaccgga aggcggcctg
    16381 gaagctggag cgtttcatca tcaactccgg caactccata acctccacca gcgcggtcag
    16441 cctggcttcc cgggacggca ggaaggagga gcgggtggcc accggggacg gacccatcga
    16501 cgcgtccttc aaggccatcg acaagatcgt gggccgggat ccggtgctgg aggatttctc
    16561 cctggattcc gtcaccggcg gcaaggacgc ccagggtgag gcccacgtgc gggtgcgcct
    16621 gggggacaga gcttacaacg ggcacggggt gtccacggac gtcatcgagg ccagcgtccg
    16681 ggcctatatc gacgccatca acgccatggc ggaggacctg gacgcggagg gtggcccggg
    16741 cccttcctcc gccggggcgg aggaggacgc cgatggacag gcttccgcgc gtaacgcttc
    16801 ttgattccac cctccgggac ggggcccagg gcgagggggt gagcttctcg gtgacggaca
    16861 agatctccgt agtccgggcc ctggacgcct tgggggtgga catcatcgag gcgggcaacc
    16921 ccgggtcgaa ccccaaggac gcggagtttt tccgggaggc ccggggcctg cgcttggcgc
    16981 ggtcgaccct ggccgccttc ggctccaccc ggcgcaggga cacccgtccc gaatcggacg
    17041 cccagttggc gagcctcctg gccgcggaca ccgggatcgt cgtcctcttc gggaagtcct
    17101 ggactttcca cgtccgcgcc gtcctggggg tctcgctcga ggagaacctg gcgatgatcg
    17161 gcgacagcgt ggccttcctg cgctccgccg ggcgtcgggt gatcttcgac gcggagcact
    17221 tcttcgacgg cttcgcggcg gacccggact atgccctggc ctgcctccgg tccgcccggg
    17281 acgccggggc ggaatgcatg gtgctctgcg acaccaacgg gggaagcctt ccgtcccggg
    17341 tggcggagtt cacccggcgg gctgccgtcg tcggcgcgcc cctgggaatc cacgcccaca
    17401 acgattcggg cctcggagtg gccaactccc tgaccgccgt cgaggcaggc gccgtgcacg
    17461 tccagggcac cctggcgggc ttcggggagc gctgcgggaa cgccaacctc tccaccgtgg
    17521 cggcggacct ccagctcaag atgggacggg actgcctggg cccgggcgcc ctggccggct
    17581 tggccggggc ggtgcgtcgg gtgacggaga tcgccaacct caccccggac gagggcatgc
    17641 cctacgtcgg ccgccgggcc ttcgcccaca aggcggggat gcacgtggac gcggtccgga
    17701 aggatccggc ctccttcgag cacgttcccc cgggggccgt gggcaacgag cgacggttcc
    17761 tggcttccga ggtgggaggc cggtccgtgt tgatggaccg gttccggagg atcctgccgg
    17821 acgccgccaa ggattcgccc gaggtggtcc gggccgcgga agcgctcaag aagatggagg
    17881 ggttggggta tcagtacgag ggggccgagg ccagcctgga aatcctcctg cgcaaggccg
    17941 ccggatccta tgttcccagc ttcaagcttc gccgctaccg gaccaccggg gagcaccccg
    18001 cggaggatcc ctcggcatgc gcccacgcca tggtacagct ggaggtggag ggacggcggg
    18061 agatctccgc cgccgagggg tgcggacctg tccacgccct ggacctcgcc ctgcggaagg
    18121 ccctggtcgg gttctatccc tccctggccg ggatgcgcct cgcggactac aaggtccggg
    18181 tcctgcccgg cacgggcgcg acggcctcgg tggtgcgggt gctgatcgac agctccgatt
    18241 cccgggacgt ctggaccacc gtcggggtgt ccgcggacat cctggaggcc agctggatcg
    18301 ccctggtgga ttcctacgag tacaagctta gcaaggacac gcaatccgag acccgacggg
    18361 agactggaga cgaacatgcc gatgacgatg acgcagaaga tcttggccgc gcatgcgggg
    18421 cttgagtccg tggagccggg gcagcttctc gaggcggggc tcgacctggt cctggggaac
    18481 gacatcacag cgcccccggc gatccgggag ttccggaaga tgggcgcccg tacggtcttc
    18541 gaccgggatc gggtggtcct ggtgccggac cacttcgcgc cgaacaagga catcaaggcc
    18601 gccgagcagt gcaagtgcat gcgggatttc gcccgggaac aggaggtcac ccactacttc
    18661 gaggtgggcg agatggggat cgagcacgcc ctcctgcccg aacgggggct cgtgctcccg
    18721 ggagacctgg tgatcggggc ggactcccat acctgcacct acggggccgc aggcgccttc
    18781 tccacggggg tggggtccac ggacatggcc gcgggcatgg ctctggggcg gctctggttc
    18841 aaggtccccg aggcgatacg ggtcgtcctg accgggaagc cttcggggtg ggccgggggc
    18901 aaggacctca tcctgaccct gatcgggatg atcggggtgg acggcgccct ctatcaatcg
    18961 ctggaattcc agggggccgg ggtggctgcc ctcacgatgg aagagcgcct gacggtgtcc
    19021 aacatggcca tcgaggcggg ggccaagaac gggatcttcc cggtggacga ggccacccgc
    19081 gcctgggcgg cctccgtttc ggagcggccg taccgggagt accgggcgga tccggacgcc
    19141 gtctacgccc gggaggtccg gatcgacctg tcccggctcg tcccggtggt cgccttcccc
    19201 catctgccgt ccaacgtccg gccggcgtcg gaggcggggg acgtcccggt ggaccaggtg
    19261 gtgatcggat cctgcacgaa cggccggatc gaggacctca ggatcgcggc ggccatcctc
    19321 cggggccgga gggtggcccg gaccgtccgg tgcatcgtca tcccggccac ccagaggatc
    19381 tacctccagg cgatccggga aggcctggcg gaggacttcg tggaggccgg ggccgtactg
    19441 tccaccccga cctgcgggcc ctgcctgggg ggccacatgg gcatcctggc cgcgggggag
    19501 cgggccgtgg ccacgacgaa ccgcaacttc gtgggccgca tgggccatcc cgagtcggag
    19561 atctacctgg cgggtcccgc cgtggccgcg gccgccgcgg tgacgggccg gatccgcgac
    19621 ccctcggagg tcctggaagc cccggaccgg gagcggctcc tctcgacgcc ggagcccggg
    19681 tggggcggcg gccgggccgc gagccgcggc aggtcccggg atgccgtccc ggcgggaggc
    19741 gcacgatgac cgccgcggga cgtgccttca agtacgggga caacgtggac accgacgcca
    19801 tcatccccgc ccgatacctc aataccagcg atcccgcaga gttggccgcc cactgcatgg
    19861 aggacctgga cccctccttc gcgggggcgg tgcggcccgg ggacgtcctc gtggggggcc
    19921 ggaacttcgg ttgcggctcc agccgggagc atgccccggt ggctatcaag gccgcaggag
    19981 tctcctgcgt gatcgcggct tccttcgccc ggatcttcta ccgcaacgcg gtgaacatcg
    20041 ggcttcccat cctggaatcc cccgaggccg ccgccgggat cgaggccggg gaccggctgt
    20101 ccctggattt cggatcggga accatctcgg acgggacgaa gaactcctcc tggcggtccg
    20161 cccccttccc gcccttcatg cggcgcatca tggaagccgg gggcctcacg gagtacgtgc
    20221 gcgcccgtct gaccgaggcc ggggattccg gcgccgaggg aggcgggtca tgaagaacgc
    20281 cataaccctg gtgcccgggg acgggatcgg cctcgaggtg accgcccagg cggtccgggt
    20341 cctggaggct gtcggggccc agttcggcgt ctccttccgc ttcgagaccg tcctcctggg
    20401 gggcgcggcc atcgacgaga agggagttcc cctgcccgcg gagaccctgg cggcctgcaa
    20461 ggccgcggac gcggtgctcc tgggcgcggt cggggggccc cgctgggacg acctttccgg
    20521 agcccgcaga cccgaggccg ggctcctggg gctccgggct ggcctggggg tgtacgccaa
    20581 cctcaggccc gcccggcttt tcccggaact ccgctccgcc tgtcccttga aggacgagat
    20641 cgcggggaag ggcttcgacc tcctgatcgt gcgggagctc accgggggca tgtacttcgg
    20701 ggaccggggc cgcagtcccg acggggaggt ggcctgggat acggagaggt attcccgcaa
    20761 ggaaatcacc cgcctcctga ccatggcctt ccgggccgcc cggagccggt ccggaaggct
    20821 ctgcgtggtg gacaaggcca acgtcctgga gtccagccgc ctctggcggg agacctcccg
    20881 agctctgtcc cccgcttggc cggacgtgga ggtctcgta
//
LOCUS       DAOTEI010000043        20834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1680_length_20834_cov_4.810963, whole genome shotgun sequence.
ACCESSION   DAOTEI010000043 DAOTEI010000000
VERSION     DAOTEI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 20834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20834
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1680_length_20834_cov_4.810963"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            684..1463
                     /locus_tag="P5313_06125"
     CDS             684..1463
                     /locus_tag="P5313_06125"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRY79973.1"
                     /translation="MNLLVLEPREIGTPLPRSDRRVEHTRKILKKGVGDVLAAGLAGG
                     PGDPCPLGTARVESWDGSRMVFSFAPEREAPPLRPVSLILGFPRPIQVTRILKDLCSL
                     GVRRILMAPTDLGEKSYADSTIFKERDFLRPLLEGAEQAGNPRLPEADAFPSLAAALA
                     ALGSAAPGETRWALDPYRAEGAFGSLLDFGAGTPGPGPSGSASRAFTLAVGSERGWTD
                     RELDALAASAFRFASLGDRILRTETACAVAVTLALASLGRL"
     gene            complement(1460..3259)
                     /gene="pepF"
                     /locus_tag="P5313_06130"
     CDS             complement(1460..3259)
                     /gene="pepF"
                     /locus_tag="P5313_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRY79974.1"
                     /translation="MAANDIPVRKDVRPEDRWNLSKLFPDDAAWERGLSDLKSRIPRI
                     ASFKGTLARSPESLLAALAFFRDYGILEERLGYYADLRQTEDEGDSENRARAGRYMMA
                     ATEGQGEWSWLVPEIQRIPDESLEAWLGREDFAEYRVFLRKLIRFKPHVLSEPEERLL
                     ALQAEAAQTAQETFSVLTNVDLDFGEVETPEGPRKLSQSTFASLLRHADREVRRKAYL
                     QFYGSFEAHKTTLAQLYAGSCKLDKYRAQVRNFPSARAAALFPDDVSEVVYDNLVSAV
                     GASLPALHRYYSLRKRVLGVSELRHYDVYVPLVPAAKARYTYDEAVEVVCAALAPLGP
                     EYVDTLRAGLTGAWVDRYENKGKRSGAFSAGSFTGDPYILLNYKEDVLRDVFTLAHEG
                     GHSMHSYYSARNNPFLQYNYTIFEAEVASTFNEELLFEYMYRNADSKALKAYLVNTKV
                     DDLLATLHRQTMFAEFERLSHAALEGGTPLTVDFLRAEYRKLLEKYFGPDLVFEPTSD
                     LEGLRIPHFYNAFYVYKYSTGVSAAAALARRVLEGGKAERDAYFAFLKSGGSRFPIES
                     LKLAGVDMSTPGPVEAACAEFARLVDELEGLLA"
     gene            3412..4896
                     /gene="zwf"
                     /locus_tag="P5313_06135"
     CDS             3412..4896
                     /gene="zwf"
                     /locus_tag="P5313_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRY79975.1"
                     /translation="MDTGLRCDLDSERPAPGPAMIVVFGGTGDLARRKIVPALYNLLR
                     EGRLHPDSRILCAGRRALDPREYREGLREAAARFSRTGLDPAAWDRLAARIEYREGDL
                     DSPALYDSLARTLSSPGSPADALFYLAVSPEQFGPAARGLARAGLGTAGRHPDDTAPY
                     RRLIVEKPFGRDRASARALNLELQDCFAERDIFRIDHYLGKETVQNLLYLRFANSVFE
                     PLWNRRYVERVEIDVLEEEGIGTRGGYYDGAGAARDMLQNHLVQLLCLTAMEPPAGLD
                     PESIRDEKVKVLKSIPAYSPEELRRRSLRGQYGAGSDAAGRPLRGYREEDRVRPGSRT
                     ETFAALRLEVDNWRFAGVPFVLRTGKALDRRVSEIRVLFRRPPGTLFQGSCGDRLGPN
                     GLILRIQPDEGLWLTFNAKVPGRAAVESGALRFSYREKADSLPEAYERLLADALAGDS
                     TLFIRSDETEAAWSLVDRLEEAWADPAAAPPVLYPAGSPPPAAP"
     gene            4904..5665
                     /locus_tag="P5313_06140"
     CDS             4904..5665
                     /locus_tag="P5313_06140"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRY79976.1"
                     /translation="MEILRFPTEEAWIEAARTAFRTAAAAAKLRGSDTLRLCLAGGRT
                     PEPVYRALARIPLRDVRVELWLGDEREVDPASPDRNGGLVLRAFRDCAWDPAPVFLLW
                     PGGPGTEACRAVEARLAGLAEPIFDLSFLGIGEDGHTAGIFPGGPEAVETSRLAVPSR
                     APSEPRARMTLSPRALASTRTLLYLLRGPAKLGLAERLAAGPVPDGRAAVEYPPDGPR
                     SGGASGTPRVLLPAELVAQRARAVGADVILSYCAT"
     gene            5740..6972
                     /locus_tag="P5313_06145"
     CDS             5740..6972
                     /locus_tag="P5313_06145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY79977.1"
                     /translation="METKSPADSDGSILAAVMGSAHQHLLSLGEDGTVLAFLPTETPL
                     LGRTREEVVGRKLDALFRADYVRVFDGKDAWNEIVYLEGTDGKPRPFGVRRIPADREP
                     FPALVPGAREHLLVNELLIYGALEDLHERTLAEHAQELEAVNEKLKRQTHQLRKALEI
                     LESRNRQMIKELNLAVELQKSLLPKSYPPTPSAVFTHRYIPLAMVGGDFFALSKMEGD
                     RVGILIADVSGHGVAPAFITAMIRSLFDYLHPREDSPAEVIRRINEEFSKIVNTDHFV
                     TAFYGIFDFEAMSCTYCGAGHPPQLIVHSDGTVTRLASEDPIIGMTEDCDYHDRSAAL
                     AGGDLLCFYTDGVQEARNAQDEMFGVRGIEETVIRNRNSDLEAVADALITGVIQFMKD
                     PFFEDDITVVLGQVIESL"
     gene            complement(7141..8034)
                     /locus_tag="P5313_06150"
     CDS             complement(7141..8034)
                     /locus_tag="P5313_06150"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRY79978.1"
                     /translation="MIPSLFLRALTVPDADRPAVLEALLRDRGVASARIPLAGSLHLA
                     VKFPDSSYNPRYRTKLLVAHHDRRPGSSGALDNGAACLQLADLAGRLALREEPHNTVI
                     LFTDSEESSGAGDQGSFAVARALAGRVKALAGEGEEPPAVFVFDVTGRGTVPILSTTA
                     RDLLASRGPDCGDLAGRIGDLESWAARTLRRAGAAPLRLPVPWSDDLGFALGGIPAVT
                     VTLLPRSELDARSPAPRHGRPDGTQAPHGEFPAEPPATWALLHGPGDRPEILEEESFD
                     LMARILDAFGDLRIPRVRRIR"
     gene            8216..8470
                     /locus_tag="P5313_06155"
     CDS             8216..8470
                     /locus_tag="P5313_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79979.1"
                     /translation="MDSIGTWRILSLLETHGEEEVYSATDSADEDLRLRILHPKAPEE
                     DKIAFEEMERTEEATLEGSRRIREIGRTEDGRPYAVYSAL"
     gene            8575..10467
                     /locus_tag="P5313_06160"
     CDS             8575..10467
                     /locus_tag="P5313_06160"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRY79980.1"
                     /translation="MKHPAKPRPAAESGEPVPIQPSAAPGPEVKRPGIAALAYAGILV
                     GSVPLTGFSRLDGLYVVYLLLTLAVAAGELAFLSAIFRKGRTGLFPAWKKFTGYSIPV
                     YYFAHALPRIEWSNAGLIGPGRVLFLFILTLAAAAAGGLAFFLAGRPGALAAAGVIES
                     EEVSDRALRKRRARERKPRGLVAVVLDWVDAIAWAAIAVLVVNIFVFQLYEVPSESMV
                     PAFLVKDRPFTVKLSSGPRVPLTEWRLPFLRLPRRGDVVTISNPRYPENHRVDLKKHV
                     SQFVYMVTLTAVHLDSTLPDGTPKADPLVKRITGVPGEKLMMVDDVLYARRKGEPGFR
                     KVEEPWAATDLWKQDAALRRKIQHLPLDEATRALLARVDALKNGAKPEDLGAAVGSSL
                     GRIEAALSRLEASGREAASGPDMSRTDPALRTRRDEAASAAARGENPFAEAGAAAEDL
                     SLALAASASPAVRSALRAYAEGAAAAAAAPAANAYDRGSRSLNLLVKANLLARMERVL
                     GLLAAGTPLETAARDPERGRLVAEARELYLYLQGFYDNRNFPEFPAGEAFLAPDEYFA
                     MGDNRYNSLDFRFRETPATRALDPADPASVRYVSILEPFAVKLKFIEGHAVFRLWPPS
                     RLGIIR"
     gene            10487..12142
                     /locus_tag="P5313_06165"
     CDS             10487..12142
                     /locus_tag="P5313_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRY79981.1"
                     /translation="MKEIRVTYPDGRVLSYPFLIKASDALEPLGTLPRPLAAALINNE
                     LCPLDYFLTTDCTLAPVTVDTNLGAVTYRRSLCFLLAMASRELFPNRRLVAGMAIGTG
                     FFHFFDDDEKDDPKDIRILEKRMRELVARDIPIHREWMSYSGALEYFRTQGQTDTLAL
                     LEHMNDPRIAVNECAGFRDLHVTPLVPSTGVLAVFELLPYHDGFLLRYPHKEDDKVPP
                     FEDDPVLYRIATEYRERGKILGVSSVGTLNSINSPKKIKEYIQVAEALQNKKMAELAD
                     QVAARAGTVKALLIAGPSSSGKTTTSKKLAIQLKVMGFEPVVIALDDYFVDRDHTPRD
                     ENGEYDFECLEALDVEYLNEQLVELFRGDEIELPAFDFKTGTRRPSGKKLRLSTGREI
                     VVMEGIHGLNDKLTPRIPREQKFKVYVSALTQLNLDDHNRISTTDNRLLRRIVRDWQF
                     RGKTALDTLKMWASVQRGERRHIFPFQNSADAAFNSALDYELGVLKIYAEPVLRSVKP
                     IEPEYAEAGRLLAFLANFSPIPAQFVPGNSILREFIGESEFKY"
     gene            12354..14918
                     /locus_tag="P5313_06170"
     CDS             12354..14918
                     /locus_tag="P5313_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY79982.1"
                     /translation="MSRKVLIILAIVTAAAFALAGCGGGAGAGAGVAKGDTGPLVDKI
                     IIDVRMDQSIAAKDTVEGKTDIFAYGMDASVFFGLPQADREKLSVYPVPSGSWSYLMN
                     PIPNQAPYVWKTKTGKEYFNPLAIREVRYAINWLIDRKKMVDEILLGGGEPAFTSMTP
                     GQPGTYRYNIIATKLGMTERGDEKRAIAEITAALEAAAKLPENSGKLRKTGQFWTYKG
                     EPIVVKFIIRVDDPQGRLPAGRYVADQLEKAGIKVERLEYDRSKAGRMVYGVDPAAYE
                     WTMYTEGWGAGATRRWWDVSISQMYAPYYGYMPGGATEGFWNYKQDEIDKLAMKSYNG
                     WFLTDEEYWNDNLKAQELGLTEAVRIWVCSQIDYFIANKDRVTNRFVYGLGDGLNGWS
                     FITADVKPNDKGEKILRATQFSAKGGLFMSAWDPVGVDGFSDTYVSMIVEQCTMREYF
                     EAPNTAIDTPYLVQWDLKDVQSNVGPDTTGDGKPEGLIDVPADAVKYDSATKTWKKVG
                     SGVKSYSTAKYTVKDSVWHDGNKIGLADFVYSLGFQTDWSTKDGDADLAYEEAYASQY
                     QPTLETLKGVIFNKDGSFTTYYDFNWPMDKNRVAYGGLLSPKAGNPGRPTMVPWTITE
                     AIGLLVTEGAASGTVYAITEDPSMTQIDVASPACVADLKAKLNEMVEKKHVPVYVKDH
                     MKPAEAVDAYKKTLAFIEKYGHAYVSNGPFYISKIDSTANYMELSAYRKGYPYKKDYW
                     GKFFALEVTQIDNVAIPANARRDADAVIDLTVSQFTYPSDQTKPADKDAKVQVTLISQ
                     DGEKAYPAKYVKDGSFQAVIPAADLAKLQPGSYTLVSESKFADEAPSVKPGNLVLF"
     gene            15056..16096
                     /locus_tag="P5313_06175"
     CDS             15056..16096
                     /locus_tag="P5313_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79983.1"
                     /translation="MYKWYAIKRILKGVAMYALLMFTFSALFNTVSETTLKGQIEEQV
                     RQESQRLRNLKSEQIRAFQIDRRDQLYKLYKLDRPWTERVVSRAVNALTFKFGRSTII
                     KTQKGEREVIKIIMEAMPRSILLFTTTIVFEIFLGIWLGLKKAQKPGGSLDKSTSFIT
                     MVVYGLPSWWLGMMLIMIFVYGIKLFPSGGLHSVPTPGGILKVLDLIWHMILPMLTLL
                     LIGFWSLGFVVRNIVLGVLQEDYIMSARARGIPENKVLFGHTMRTAAPPLITMALLTL
                     LASISGSIIFEGIFSWPGLGNLYWIAIQQNDIPVLLADLAITTGIYQAGLIFLDLTYG
                     FLDPRIKIGGKA"
     gene            16093..17487
                     /locus_tag="P5313_06180"
     CDS             16093..17487
                     /locus_tag="P5313_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY79984.1"
                     /translation="MKLEDAAYRLKEFWGEFRKERNGLVGLGILIAAVLAVLLEPLIL
                     PFPEASSRWRDITYWQDNSKSAPPAWTNLFTARKSAVALTLWDSRTEDRGPGEKAYIF
                     SYDYEFSRAPYDLLLRGEGSGTIPLTVTVRRPDSNAVEIIRYVLQDLAGQTFRLSFTN
                     EGKANALEFLRKTERPENAAQIATMSYGVPEILFSRAEPDFVKGPKPLAGRYEIEVTA
                     GFTDPRASIEHMEIVVVGRVSGILGTDSMKRDIFSGVVAGMKWALMIGLLTSIVSVLV
                     GVFYGITAAYFGGRVDSVMMLIFDYFISLPVLPILIVFSAVYKPSIWTIIAALILFFW
                     VGPVKTVRSMALQIKEETYIEAAKALGAGHGRIIFRHMAPLLIPYSFASMALAVPGAI
                     VYESSVSLLGLGDATIVSWGQILHDALGSGAVLSGLWWWVVPPGLLIAVMGMTFAFLG
                     FAMDKILHPKLKTR"
     gene            17491..18462
                     /locus_tag="P5313_06185"
     CDS             17491..18462
                     /locus_tag="P5313_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79985.1"
                     /translation="MSETVLQVKNLKLYYHTSKGAVKALDDVSFDLKAGETLGLVGES
                     GCGKTTMGTGLLRMPTPPGRFEGGQILVEGRDILPLPEKEIRKDFRWEKVSMVFQGAM
                     NCLTPVYTIGKQMMETLQEHRSMEKAEAQELIKEYLSYVGLPADVTSRYPHELSGGMK
                     QRVVIATALFLKPRLVILDEPTTALDVIVQAQIINLLKKLKKTFDLSFIFITHDLALE
                     AEVSDRICVMYAGKIAELGTNEQVYGGKGPAHPYTERLLAATPRLRRKVESLSFIPGT
                     PPDLIAPPSGCRFHPRCPRVYERCRMEEPPVREIEPGHLAACWRCFE"
     gene            18459..19478
                     /locus_tag="P5313_06190"
     CDS             18459..19478
                     /locus_tag="P5313_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY79986.1"
                     /translation="MSNGIDGDVILRVDDLKKHFTPHQGFLQSFGKVRKVVRAVDGIS
                     FEIRRGEIFGLIGESGSGKTTTGKLVMKLIDATSGRILFNGRDVTSIGHEETKEYRRQ
                     VQMIFQDPYASMNPRFKIRDVLEEPLEIHRITADRKERETRIVRALTEVKLTPPEDFM
                     GRFPHMLSGGQRQRVATARTLILNPAMLVADEPVSMIDLSTRAEILHMMKDVQRDLGL
                     TYLYITHDLSTARYFTDRIAVMYLGRIVEMGKADDIIDHPAHPYTRALIEAVPEPATG
                     KVDVVKELPISGEIPSPSNIPAGCRFHTRCPYMKPECKEEAEPELMDLGSGHYHACRR
                     WKEIG"
     gene            complement(19560..20312)
                     /locus_tag="P5313_06195"
     CDS             complement(19560..20312)
                     /locus_tag="P5313_06195"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HRY79987.1"
                     /translation="MTDLLKEGDREESRPGAIPSFRSVLSLGVLAALALTGCLSLLAV
                     RVALLGNPFYTFLGMNLLLACVPLGMAAVQVILLRTLNPGPARTVLVSVCALGWLLFF
                     PNAPYLFTDFIHVAEKTWIRSDTIQWLGTRGVLWYDIVMSGAFAFVGHFLGLVSMWVE
                     GRVLDHAWGRGFERVFLTVAVILAGFGVYLGRFVRLNSWDAVLTPVRAVRGTLAALSD
                     PKAVLFSLTFSFFVAVTYGMLVLAFSLRDPPK"
     gene            complement(20339..>20834)
                     /locus_tag="P5313_06200"
     CDS             complement(20339..>20834)
                     /locus_tag="P5313_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRY79988.1"
                     /translation="REWQLTTIQFDFNEPERFDMTYVDADGQRKRPYMVHRALLGSVE
                     RFFGVYLEHTAGAFPIWLSPEQAVVVPVAPAFDEYAKKVAGELAAAGLRARADLSDDR
                     MNAKIRNAQNSKIPYILVVGQKEAEEGAVAVRLRDGTQLGALKIADFTDRVLRKVRAK
                     ALDL"
BASE COUNT         3630 a         7266 c         6690 g         3248 t
ORIGIN      
        1 gccggatccg cctggacgac ctcgagatga gcccggagat ccagtcggag accacccgca
       61 ggatgatgac cgtgacggaa gagaacgccg aggccgtgac ggacgccgag ggcttccgga
      121 cggacttcct ccaggctcac ggttttgctg tacccggcgt ggactacggg gaggactgat
      181 gtaccgcggg cggcccgtgc cgcccgcccg tgaaccgagc accgcacgct ttcccggccg
      241 ccgggaaagc cttgccggtg aggcctccgc cccgggcggg atcttcaccg gccagccgcg
      301 gccgcgcccg gaccgtcgag gaggaggcag cccagaacca acatgaaccc acaaggatac
      361 caacccgtag attgtccgga gcccggatac ctccggggct cgacggcggg cgaacggcgg
      421 caaaaaagga gacagtcccc aggatccagg actcagatga cgggagccgg gtgtcgggac
      481 tcgggggacg ggaacccggg agagccggac ccggaccacc ccgggtcctt cctgtcggat
      541 gccccggaag ccggaaatca ggctgattct acgaaacttc acataaaacc atactgaaca
      601 atattatctg aactcacccc gtattctctc ttccgtccac tgtccactgt ccactgtcca
      661 ccgtccaccg ctatactccc cccatgaacc tcctggtcct ggaaccccgc gaaatcggaa
      721 cccccctgcc ccgctccgat cgacgggtgg agcacacgag gaaaatcctc aaaaaagggg
      781 tcggcgacgt tctggccgcg ggactcgcgg gaggacccgg ggatccctgc cccctgggca
      841 cggcccgggt ggaatcctgg gacgggagcc gcatggtctt ctccttcgca cccgagcggg
      901 aggccccgcc cctgcgcccg gtctccctga tcctcggatt cccccggccc atccaggtga
      961 cccgcatcct caaggacctg tgcagcctgg gggtccgccg catcctcatg gcccccacgg
     1021 acctggggga gaaatcctac gcggacagta cgatcttcaa ggagcgggac ttcctccgcc
     1081 cccttttgga gggggcggag caggcgggga atccccggct tcctgaggcg gatgccttcc
     1141 cgtccttggc cgccgccctg gcggccctgg gctccgccgc acccggggaa acccggtggg
     1201 ccctggatcc gtaccgggcg gagggagcct tcgggagcct cctggacttc ggcgcgggca
     1261 ctccgggccc cggtccttcg ggatcggcct cccgggcctt caccttggcc gtgggctccg
     1321 agcggggctg gacggaccgg gaactcgacg ccctggccgc ctccgccttc cgcttcgcct
     1381 ccctggggga ccgtatcctc cgcaccgaga ccgcctgcgc cgtcgcggtg accctcgccc
     1441 tcgcctccct cggccggctt taagccagaa gcccctccag ttcgtccacc aggcgggcga
     1501 actcggcgca ggccgcttcc acgggccccg gggtggacat gtccaccccc gccagcttca
     1561 gggactcgat ggggaagcgg gaccccccgg acttgaggaa ggcgaagtag gcgtcccgct
     1621 ccgccttccc gccctccagg accctccggg ccagggccgc tgccgcggac accccggtgg
     1681 agtacttgta gacgtagaag gcgttgtaga agtggggaat ccgcaggccc tccaggtcgc
     1741 tcgtcggctc gaagaccagg tccggcccga agtatttctc aaggagtttc cggtattccg
     1801 cccgcaggaa atccaccgtc aggggggtgc cgccctccag ggcggcgtgg gagagccgct
     1861 cgaactcggc gaacatggtc tgccggtgca gggtcgccag aaggtcgtcg accttggtgt
     1921 tcaccagata ggccttgagg gccttggaat ccgcgttccg gtacatgtac tcgaacagga
     1981 gttcctcgtt gaaggtgctg gccacctcgg cctcgaagat cgtgtagttg tactggagga
     2041 agggattgtt ccgggcggaa tagtaggagt gcatggaatg tccgccctcg tgggccaggg
     2101 tgaagacgtc ccggagcacg tcctccttgt agttcaggag gatgtaggga tcccccgtga
     2161 aggagcccgc cgagaaggcc ccgctccgct tgcccttgtt ctcgtagcgg tccacccagg
     2221 cgcccgtgag gcccgcgcgg agggtgtcca cgtactcggg acccaggggg gccagggccg
     2281 cgcagaccac ctccacggcc tcgtcgtagg tgtaccgggc cttggccgcg ggcaccaggg
     2341 ggacgtagac gtcgtagtga cggagctccg aaacgcccag gacccgcttg cgcagggagt
     2401 agtaccggtg cagggccggc aggctggccc cgacagcgga cacgaggttg tcgtagacaa
     2461 cctcggagac gtcgtccgga aagagggccg ccgcccgggc ggaggggaag ttccggacct
     2521 gggcccggta cttgtccagc ttgcaggagc ccgcgtagag ctgggccagg gtggtcttgt
     2581 gggcctcgaa ggatccgtag aactggagat aggccttgcg ccgcacctcc cggtccgcgt
     2641 gtcggaggag ggaggcgaag gtggactggc tgagcttgcg gggcccctcg ggagtctcga
     2701 cctccccgaa atccaggtcc acgttcgtga ggaccgagaa ggtctcctgg gcggtctgtg
     2761 cggcttcggc ctggagggcc aggaggcgct cctcgggctc cgagaggacg tggggcttga
     2821 agcggatcag cttgcggagg aagacccggt actccgcgaa gtcctcgcgt ccgagccagg
     2881 cttccaggga ctcgtccggt atcctctgga tctccggaac cagccagctc cactcgccct
     2941 gtccctccgt ggcggccatc atgtaccggc cggcccgggc gcggttctcg gaatccccct
     3001 cgtcctcggt ctgccggaga tcggcgtagt accccagacg ctcctccagg atgccgtagt
     3061 cccggaagaa ggccagggcc gcgaggaggc tctccggtga ccgggccagg gttcccttga
     3121 aggaggcgat gcggggtatg cgggacttga ggtccgagag tccccgctcc caggcggcgt
     3181 catccggaaa gagcttcgaa aggttccagc ggtcctcggg gcggacatcc ttgcgcacgg
     3241 gtatatcgtt cgctgccatg gtcatcctcc tggttcgatt cgggcctggg tcgatcgggt
     3301 gagtatacac caccgggcgc gtttatccac ggcgggattg acaccccgcg gcggggggac
     3361 gaacattccc gggatacgac tccacgaaag gcggaccgga tgcgggaagg catggataca
     3421 ggactccgct gcgacctgga ttcggagcgg cccgccccgg gcccggcgat gatcgtcgtc
     3481 ttcggcggga ccggggacct ggcgcgccgg aagatcgtac ccgccctgta caacctcctg
     3541 cgcgaggggc ggctccaccc ggactcccgg atcctctgcg cgggccggag ggccctggat
     3601 ccccgcgaat accgggaggg ccttcgggag gccgccgcac ggttctcccg gaccggattg
     3661 gaccccgcgg cctgggaccg cctggccgcc cggatcgaat accgggaggg cgacctcgat
     3721 tccccggccc tctacgattc cctggcgcgg acgctctcct ccccgggatc gcccgcggac
     3781 gccctcttct acctggcggt ctctccggag cagttcggac ccgcggcccg gggactggcc
     3841 cgggcgggcc tgggcaccgc gggccggcac cccgacgaca cggcgcccta ccggcgcctg
     3901 atcgtggaaa agcccttcgg ccgcgaccgg gcgagcgccc gggccctcaa cctggagctc
     3961 caggactgct tcgcggagcg ggacatcttc cggatcgacc actacctggg gaaggagacg
     4021 gtccagaacc tcctgtacct gcgcttcgcc aactccgtgt tcgagcccct gtggaaccgg
     4081 cgctacgtgg agagggtgga gatcgacgtg ctcgaggagg agggaatcgg cacccggggg
     4141 ggctactacg atggggcggg ggccgcccgg gacatgctcc agaaccacct ggtccagctg
     4201 ctctgcctca ccgccatgga gccccccgcg gggctcgatc cggagtccat ccgggacgag
     4261 aaggtcaagg tcctgaagtc catccccgcc tattccccgg aggagctccg aaggcgtagc
     4321 ctgcgcggcc agtacggggc cggatccgac gccgcgggcc gccccctccg gggctaccgg
     4381 gaggaggacc gtgtacgccc cgggtcccgg accgagacct tcgcggccct ccgcctggag
     4441 gtggacaact ggcgcttcgc gggggtgccc ttcgtcctcc gcacgggcaa ggccctggac
     4501 cgccgggtca gcgagatccg ggtcctcttc cgcaggcccc cggggaccct cttccagggc
     4561 agctgcgggg accgcctggg tcccaacggc ctcatcctcc ggatccagcc cgacgagggt
     4621 ctctggctga ccttcaacgc caaggtcccc ggccgggccg cagtggaaag cggggccctc
     4681 cggttctcct accgggagaa ggccgattcc ctgcccgagg cctacgaacg gctcctggcc
     4741 gacgccctgg ccggggactc caccctcttc atccggtccg acgagaccga ggccgcctgg
     4801 tccctggtgg accgcctgga ggaggcctgg gcggacccgg ccgccgcgcc gccggtcctc
     4861 tatcccgcgg gctccccgcc ccccgcggcg ccctgaggcc cccgtggaga tcctgcggtt
     4921 tcctaccgag gaggcgtgga tcgaggccgc ccggaccgcc ttccggacgg cggccgccgc
     4981 cgcgaagctc cgcggatcgg acaccctccg gctctgcctg gccggaggcc ggaccccgga
     5041 gcccgtgtac cgggccctgg cccggatccc cctccgggac gtgcgggtcg agctctggct
     5101 cggggacgaa cgggaggtcg acccggcctc cccggaccgc aacgggggcc tcgtccttcg
     5161 ggccttccgg gactgcgcct gggatccggc cccggtcttc ttgctttggc cgggaggtcc
     5221 cggcacggag gcctgccggg ccgtggaagc ccggctcgcg gggctggccg agcccatttt
     5281 cgacctgtcc ttcctgggaa tcggcgagga cggccacacc gccgggatct tccccggggg
     5341 tcccgaggcc gttgagacgt cgaggctggc cgtgccgagc cgggctccct cggaaccccg
     5401 ggcccggatg accctctccc cccgcgccct ggcctccacc cggacgctcc tctatctcct
     5461 ccgggggccg gccaagctcg gactggcgga acgtttggcg gcaggacccg tcccggacgg
     5521 acgagcggct gtggagtacc cgccggacgg gccgcgctcc ggcggagcct ccgggacgcc
     5581 ccgggtactc ttgcccgcgg agctcgtggc gcagcgggcc cgggccgtcg gcgccgacgt
     5641 gatcctgtcg tactgcgcga cctgaaaccc gatcccccgg gctcgataaa tggcccgaac
     5701 gtctttcaaa tctccgggcc gcgtcctaca attaccccga tggaaaccaa gtccccggca
     5761 gattccgacg gctcgatcct ggccgcggtc atgggctccg cccaccagca cctcctgtcc
     5821 ctgggggagg acggcaccgt cctggccttc cttccgacgg aaacacctct cctgggccgg
     5881 acccgggagg aggtcgtggg gcggaagctg gacgccctgt tccgggccga ctacgtccgg
     5941 gtcttcgacg gcaaggacgc ctggaacgag atcgtctacc tggagggaac ggacgggaag
     6001 ccccgcccct tcggggtccg ccgcataccc gcggaccggg agccctttcc ggccctggta
     6061 cccggagccc gggagcatct cctggtgaac gagctcctca tctacggggc cctggaggac
     6121 cttcacgaac ggaccctggc cgagcacgcc caggagctgg aggcggtcaa cgaaaagctc
     6181 aaacgccaga cccaccagct ccgcaaggcc ctggagatcc tggaatcccg gaaccggcag
     6241 atgatcaagg agctcaacct ggcggtggag ctccagaaat ccctcctgcc caagtcctat
     6301 ccccccactc cctcggcggt cttcacccac cggtacatac ccctggccat ggtcgggggg
     6361 gacttcttcg ccctctccaa gatggagggg gaccgggtgg gaatcctcat cgccgacgta
     6421 tcggggcacg gcgtggcccc ggccttcatc accgccatga tccggagcct cttcgactac
     6481 ctgcatcccc gggaggactc gcccgcggag gtgatccgcc ggatcaacga ggagttctcc
     6541 aagatcgtga acaccgacca cttcgtcacg gccttctacg gcatcttcga tttcgaggcc
     6601 atgtcctgca cctactgcgg ggcgggccac cctcctcagc tgatcgtcca ttccgacggg
     6661 accgtgaccc gcctggcctc ggaggatccc atcatcggaa tgaccgagga ctgcgactac
     6721 cacgaccgct cggccgccct ggcgggcggg gacctcctgt gcttctacac cgacggggtc
     6781 caggaagctc ggaacgccca ggacgagatg ttcggggtcc gggggatcga ggagacggtg
     6841 atccggaacc ggaactcgga cctggaggcc gtggcggacg ccctgatcac cggggtcatc
     6901 cagttcatga aggatccctt cttcgaggac gacatcaccg tggtgctggg gcaggtcatc
     6961 gagagcctat gatcggcttt agccgatcat aggctctcgg caacgaagcg cttcgcgctt
     7021 cgtcgatctc tttaaggacc caggcgaaag ggccgactag tggtcgatga ctccgacgcc
     7081 tcagcggata ccgttcacgt caccctggta tgcctctgtc caccgaccac cgaccactgt
     7141 tcaccgtatc cgtctcaccc tcggtatcct cagatccccg aaggcgtcca ggatacgggc
     7201 catgaggtcg aaggactcct cctccaggat ctcggggcgg tcgccggggc cgtggaggag
     7261 ggcccaggtg gcggggggct ccgcgggaaa ctccccgtgc ggggcttgtg tcccgtccgg
     7321 gcgcccgtgg cgaggggcgg gactcctcgc gtccagttcg gaccgcggca ggagggtcac
     7381 ggtgaccgcg ggaatcccgc ccagggcgaa gcccaggtcg tcggaccagg gcacgggcag
     7441 gcggagggga gccgcgccgg cacggcggag ggtccgggcc gcccaggact ccaggtcgcc
     7501 gatgcgcccg gccaggtccc cgcagtccgg cccccgggag gcaagaaggt cccgggcggt
     7561 ggtggacagg atcggcacgg tgccccggcc ggtcacgtcg aagacgaaga ccgccggcgg
     7621 ctcctccccc tcccccgcca gggccttgac ccggcccgcc agggcgcgtg ccaccgcaaa
     7681 ggagccctgg tctcccgccc ccgaggactc ctcggaatcc gtgaacagga tcacggtgtt
     7741 gtgcggctcc tcccggaggg ccaggcgccc ggccaggtcc gcaagctgga ggcaggcggc
     7801 gccgttgtcc agggcgccgg agctaccggg cctgcggtca tggtgggcca cgagcagctt
     7861 ggtccggtac cggggattgt aggaggaatc cgggaatttc acagccaggt gaaggcttcc
     7921 ggccagggga atccgggcgg aagcgacccc gcgatcccgg agtaaggcct ccagcacggc
     7981 gggtcggtcg gcgtccggta cggtcagggc ccgtaggaag agggagggta tcacgctggc
     8041 ggcagtctac ccccgcgtat ccggggggtc aagggaaacg gcccttccgt gcggtgttac
     8101 cccccgcggg atcccgttgt ttcattatta gaaacattct cgccccgcgg gaagccgtcg
     8161 cccgggtggt ttcatgttcc ggccccttcc cgcccgagcg ggacaaggag gagccatgga
     8221 cagcatcgga acctggcgga tcctgtccct tctggagacg cacggggaag aggaagttta
     8281 ttccgccacg gactcggccg acgaggacct ccggcttcgg atcctccatc cgaaggctcc
     8341 ggaggaggac aagatcgcct tcgaggagat ggagcggacc gaggaagcca ccctggaggg
     8401 gtcgcgccgg atccgggaga tcggccggac cgaggacgga cggccctacg ccgtctattc
     8461 ggccctctga ggccggagga accctccggg accgggcgga accggcctgc ggacgccgtc
     8521 ccggatcggc ggcattgacg gaacggcccg ctctcttgga tactgctttc caagatgaaa
     8581 cacccagcca agccccgtcc cgccgccgaa tccggagagc cggtacccat acaaccgtcc
     8641 gccgccccgg gacccgaggt taagcgaccc ggaatcgccg ccctcgcgta cgccgggatc
     8701 ctggtcggat ccgtccctct caccggattc tcccggctcg acggcctgta cgtcgtttac
     8761 ctgctcctga ccctggccgt cgcggccggg gagttggcct tcctgagcgc gattttccgc
     8821 aagggccgga ccgggctctt cccggcctgg aagaaattca cggggtattc catcccggtc
     8881 tactacttcg cccacgccct gccgcggatc gagtggagca acgcgggcct catcggcccc
     8941 ggacgggtgc ttttcctttt catcctgacc ctcgcggccg cggccgcggg cggcctcgcc
     9001 ttcttcctgg ccggacggcc cggggccctg gccgcggcgg gggtgatcga gtccgaggag
     9061 gtctccgacc gggccttgcg gaagcgccgg gcccgggaac gcaagccccg gggcctggtc
     9121 gcggtcgtcc tggactgggt cgacgccata gcctgggcgg ccatcgccgt gctggtggtg
     9181 aacatcttcg tcttccagct ctacgaggtg ccctcggagt ccatggtgcc cgccttcctg
     9241 gtcaaggacc ggcccttcac ggtcaagctc tcctcaggtc cccgggtccc cctgaccgag
     9301 tggcgccttc ccttcctgcg cctgccgcgc cggggggacg tcgtgacgat ctccaacccc
     9361 cggtaccccg agaaccaccg ggtcgacctc aagaagcacg tctcccagtt cgtctacatg
     9421 gtcaccctga ccgcggtcca cctcgattcg accctgccgg acggaacccc caaagcggat
     9481 cccctggtca agcgcatcac gggagtgccg ggggaaaagc tcatgatggt ggacgacgtc
     9541 ctgtatgccc gccggaaggg cgagcccggg ttccggaagg tcgaggaacc ctgggccgcg
     9601 acggacctgt ggaaacagga cgcggccctg cgccgtaaaa tacagcacct gcccctggac
     9661 gaggcgaccc gggccctcct cgcccgcgtg gacgccctca agaacggagc caagcccgag
     9721 gacctgggcg cggccgtcgg atcctcgctc ggacggatcg aggcggccct gtccaggctg
     9781 gaagcctcgg gccgggaggc ggcttccgga ccggacatgt cccggacgga ccccgccctt
     9841 cgcacccgcc gggacgaggc ggcctcggct gcagcccggg gcgagaaccc cttcgcggaa
     9901 gccggagccg cggccgagga cctctccctg gctctggcgg cctccgcgtc accggcggtc
     9961 cgatcggccc tgcgcgccta tgcggaggga gcggcggccg ccgcggccgc tccggcggcg
    10021 aacgcctacg accggggctc ccggtccctg aacctccttg tgaaggccaa tctcctggcc
    10081 cggatggagc gcgtgctcgg cctgctggcg gcggggactc ccctggagac cgccgcccgg
    10141 gaccccgagc ggggccgcct ggtcgcggag gcccgggagc tctacctgta cctgcagggc
    10201 ttctacgaca accggaattt ccccgagttc cccgccgggg aggccttcct ggccccggac
    10261 gagtacttcg ccatgggcga caaccgctac aactccctgg atttccggtt ccgggaaacc
    10321 cccgcgaccc gggcgctgga tcccgcggat cccgcctcgg tccgctacgt ctcgatcctg
    10381 gaacccttcg cggtcaagct caagttcatc gagggccacg cggtcttccg gctctggccc
    10441 ccgtcccggc tgggaatcat tcgttgagat taggaggaac ctcccgatga aagagatccg
    10501 cgttacctac ccggacggcc gggtcctctc ctaccccttc ctgatcaagg cctccgacgc
    10561 cctggaaccc ctcgggaccc tcccccgccc cctggccgcg gcgctcatca acaacgagct
    10621 ctgccccctg gactacttcc tcaccaccga ctgcaccctg gcacccgtga ccgtggacac
    10681 caacctgggc gcggtcacct accgccgctc cctctgcttc ctcctggcca tggcgtcccg
    10741 ggagctcttc ccgaatcgcc gcctggtcgc gggcatggcc atcgggacgg ggttcttcca
    10801 tttcttcgac gacgacgaga aggacgaccc gaaggacatc cggatcctgg agaagcggat
    10861 gcgcgaactt gtggcccggg acataccgat ccaccgggaa tggatgtcct actcgggagc
    10921 gctggagtat ttccgcaccc agggccagac ggacaccctg gccctgctgg agcacatgaa
    10981 cgacccccgg atagccgtga acgaatgcgc cggattccgg gacctccacg taacccccct
    11041 ggtgccgtcc acgggggtcc tggccgtctt cgagcttctg ccctaccacg acggcttcct
    11101 cctgcgctac ccccacaagg aggacgacaa ggttcccccc ttcgaggacg acccggtcct
    11161 ctaccggatc gccacggagt accgggagcg aggcaagatc ctgggggtct cctccgtggg
    11221 gaccctgaac agcatcaact cccccaagaa gatcaaggag tacatccagg tagcggaagc
    11281 cctccagaac aagaagatgg ccgaattggc agaccaggtt gccgcccggg ccggcacggt
    11341 caaggccctc ctgatcgccg gcccctccag ctccgggaag accacgacct ccaagaagct
    11401 ggccatccag ctcaaggtca tgggcttcga gcccgtggtc atcgccctgg acgactactt
    11461 cgtggaccgg gaccacaccc cccgggacga gaacggggaa tacgacttcg agtgcctgga
    11521 ggccctggac gtcgagtacc tgaacgaaca gctcgtggag ctcttccggg gggacgagat
    11581 cgagctgccg gccttcgact tcaagaccgg gacccgccgg ccctcgggga agaaactgcg
    11641 gctctccacg ggccgggaga tcgtcgtcat ggagggcatc cacggcctga acgacaagct
    11701 caccccgcgg atcccccggg agcagaaatt caaggtctac gtctccgccc tgacccagct
    11761 caacctggac gaccacaacc gcatctccac cacggacaac cgcctgctgc ggcggatcgt
    11821 ccgggactgg cagttccggg gcaagaccgc cctggatacc ctcaagatgt gggcctcggt
    11881 ccagcggggc gagcggcgcc acatcttccc cttccagaac tccgcggacg cggccttcaa
    11941 ctccgccctg gactacgagc tcggggtcct caagatctac gcggaacccg tgctccgctc
    12001 cgtcaagccc atagagcccg agtacgcaga ggcgggccgc ctgctcgcgt tcctggcgaa
    12061 tttctcgccg atacccgccc agttcgtgcc cggaaactcg atcttgagag agttcattgg
    12121 ggagagcgaa ttcaagtatt gagccctacg aatcgcgccg gtactcaaat ccggggcgga
    12181 cgtttgcgca ccggcgagac cgaacgtccg gcgcgaggcg ccgggcgtat cttcatacag
    12241 taacaataga tatatcagcc ggaaccctct tcccacgccg gttccgcttt acttcccgtt
    12301 ccggatgatc cataatacgc gcaggtttct cgaattccaa aggaggagtt gagatgagca
    12361 gaaaagtgtt gatcatcctc gccatcgtca ccgcggcggc cttcgcgctg gccgggtgcg
    12421 gcggcggggc cggggcggga gccggggtcg ccaagggcga cacgggcccg ctcgtagaca
    12481 agatcatcat cgacgtccgg atggaccagt ccatcgcggc caaggatacg gtggaaggca
    12541 aaaccgacat cttcgcctac ggcatggacg ccagcgtctt cttcggcctg ccccaggcgg
    12601 accgggagaa gctctccgtc taccccgttc cgtccggatc ctggtcctat ctcatgaacc
    12661 ccatccccaa ccaggcgccc tacgtctgga agaccaagac ggggaaggaa tacttcaacc
    12721 ccctggccat ccgggaggtc cggtacgcca tcaactggct catcgaccgc aagaagatgg
    12781 tcgacgagat cctcctgggc ggcggcgagc cggccttcac ctccatgacc cccggccagc
    12841 ccggcaccta ccgctacaac atcatcgcca ccaagctggg tatgaccgag cgcggcgacg
    12901 agaagcgggc catcgcggag atcaccgcgg ccctggaagc cgcggccaaa ctccccgaga
    12961 acagcggcaa gctccgcaag accggccagt tctggaccta caagggcgag cccatcgtcg
    13021 tgaagttcat catccgggtg gacgatcccc agggccgcct ccccgcgggc cgctacgtgg
    13081 cggaccagct ggaaaaggcg ggcatcaagg tcgagcgcct ggagtacgac cgctccaagg
    13141 ccggacgcat ggtctacggc gtggatcccg cggcctatga atggaccatg tacaccgagg
    13201 gctggggcgc cggagccacc cgccgctggt gggacgtctc catctcccag atgtacgccc
    13261 cctactacgg gtacatgccc ggcggcgcca ccgagggctt ctggaactac aagcaggacg
    13321 agatcgacaa gctggccatg aagagctaca acggctggtt cctgaccgac gaggagtact
    13381 ggaacgacaa cctcaaggcc caggagttgg gccttaccga ggccgtccgc atctgggtat
    13441 gctcccagat cgactacttc atcgccaaca aggaccgggt cacgaaccgc ttcgtctacg
    13501 gcctcgggga cggcctgaac ggctggtcct tcatcaccgc ggacgtgaag cccaacgaca
    13561 agggcgagaa gattctccgg gccacccagt tctccgccaa gggaggcctc ttcatgagcg
    13621 cctgggaccc cgtgggcgtg gacggattct cggacaccta cgtttccatg atcgtggaac
    13681 agtgcaccat gcgggagtac ttcgaggcgc ccaacaccgc catcgacacc ccctacctag
    13741 tccagtggga cctcaaggac gtccagtcca acgtcggccc ggacaccacc ggggacggca
    13801 agcccgaggg cctcatcgac gtgcccgcgg acgcggtcaa gtacgattcc gccaccaaga
    13861 cctggaagaa ggtcggctcc ggcgtcaagt cctactccac cgccaagtac acggtgaagg
    13921 attcggtctg gcacgacggg aacaagatcg gcctggcgga cttcgtctac tccctgggct
    13981 tccagaccga ctggtccacc aaggacgggg acgcggacct ggcctacgag gaagcctacg
    14041 cctcccagta ccagcccacc ctggagaccc ttaagggcgt gatcttcaac aaggacggaa
    14101 gcttcaccac ctactacgac ttcaattggc cgatggacaa gaaccgcgtg gcctacggcg
    14161 gccttctgtc ccccaaggcc ggaaaccccg gacggcccac catggtgccg tggacgatca
    14221 ccgaggccat cggtctcctg gtgaccgagg gcgcggccag cggcaccgtc tacgcgatca
    14281 ccgaggatcc ctcgatgacc cagatcgacg tggccagccc ggcctgcgtg gcggatctca
    14341 aggccaagct caacgagatg gtggagaaga agcacgtccc ggtctacgtc aaggaccaca
    14401 tgaagcccgc cgaggccgtg gacgcctaca agaaaaccct ggccttcatc gagaagtacg
    14461 gccacgccta cgtctccaac gggcccttct acatctccaa gatcgactcc accgccaact
    14521 acatggagct ctccgcctac cgcaagggct acccctacaa gaaggactac tggggcaagt
    14581 tcttcgccct cgaggtgacc cagatcgaca acgtggccat cccggccaac gcccggcgcg
    14641 acgcggacgc ggtgatcgac cttacggtct cccagttcac ctaccccagc gaccagacca
    14701 agcccgccga caaggacgcc aaggtccagg tgaccctgat ttcccaggac ggcgagaagg
    14761 cctacccggc caagtacgtg aaggacggat ccttccaggc cgtgatcccc gccgccgacc
    14821 tggccaagct ccagcccggc agctataccc tggtgtccga gtccaagttt gcggacgaag
    14881 ccccctcggt gaaacccgga aacctggtcc tgttctagga cgaaggcgcg catccgaaaa
    14941 ccgcgcggcg ccctgcgggg cgccgcgctc gccttcccga gctcccggtc ggacgcgtcc
    15001 cctcgacggg cgactggtct gcggagcgaa atccttccca ggacaggtgg ccccgatgta
    15061 caagtggtac gcgataaaac gcatcctgaa gggcgtggcg atgtacgccc tcctcatgtt
    15121 caccttctcc gccctcttca acacggtctc cgagactacc ctcaagggcc agatcgagga
    15181 gcaggtgcgc caggagtccc agcggctccg gaacctgaaa tccgagcaga tccgcgcctt
    15241 ccagatcgac cggcgggacc agctctacaa gctctataaa ctcgaccggc cctggacgga
    15301 gcgggtcgtc tcccgggccg tcaacgccct gaccttcaaa ttcggccgtt ccaccatcat
    15361 caagacccag aagggcgagc gggaagtcat caagatcatc atggaagcca tgccgcggtc
    15421 catattgctc ttcacgacca ccatcgtctt cgagatattc ctcgggatct ggctgggttt
    15481 gaaaaaagcc cagaagcccg gggggtccct ggacaagtcc accagcttca tcaccatggt
    15541 cgtctacggc ctgccctcct ggtggctggg gatgatgctg atcatgatct tcgtctacgg
    15601 gatcaagctc tttccctccg ggggcctgca ttccgtgccc acacccgggg gaatcctcaa
    15661 ggtcctggac ctgatctggc acatgatcct gcccatgctg accctgctgc tcatcggatt
    15721 ctggagcctg ggcttcgtgg tacgcaatat cgtcctgggc gtgctccagg aggactacat
    15781 catgagcgcc cgcgcccggg gcatcccgga gaacaaggtg ctcttcggac acaccatgcg
    15841 aaccgcggcc ccgcccctca tcaccatggc cctgctgacc ctcctggcgt ccatctcggg
    15901 aagcatcatc ttcgaaggga tcttctcctg gcccggcttg ggcaacctgt actggatcgc
    15961 catccagcag aacgacattc cggtcctcct ggcggacctg gccataacca ccggcatata
    16021 tcaggcggga ctgatcttcc tggatctgac ctacggattc ctggaccccc gcatcaagat
    16081 aggcggcaaa gcatgaaact ggaagacgcg gcctacaggc tcaaggaatt ctggggtgag
    16141 ttccggaagg aacggaacgg cttggtcggg ctcggcatcc tgatcgcagc cgtcctggcg
    16201 gtgctcctgg aacccctgat cctgcccttc ccggaggcca gttcgcgctg gcgggatatc
    16261 acctactggc aggacaactc gaagagcgcg cctccggcat ggaccaacct cttcaccgcc
    16321 cgaaaatcgg cggtcgccct gaccctctgg gattcccgga cggaggacag ggggccgggc
    16381 gagaaagcct acatcttctc ctacgattac gaattcagcc gggcccccta cgacctcctg
    16441 ctccggggcg agggcagcgg caccattccc ctgacggtga ccgttcgcag gccggacagc
    16501 aacgccgtgg aaatcatccg ctatgtcctc caggacctgg cgggccagac cttccgactc
    16561 tccttcacca acgagggcaa ggccaacgcc ctggagttcc tgaggaagac ggagcggccg
    16621 gaaaacgccg cccagatcgc gacgatgagc tacggcgtgc cggagatcct cttctcccgg
    16681 gccgagccgg atttcgtcaa gggacccaag cccctcgcgg gccgctacga gatcgaggtg
    16741 accgcgggct tcacggatcc ccgggcctcc atcgagcaca tggagatcgt cgtggtgggt
    16801 cgggtatccg gcatcctcgg cacggactcc atgaagcggg acatcttctc cggcgtggtg
    16861 gccgggatga aatgggccct catgatcggc ctactgacca gcatcgtctc cgtcctggtc
    16921 ggggtgttct acggcatcac cgccgcctac ttcgggggcc gcgtggattc cgtgatgatg
    16981 ctgatcttcg actacttcat ctccctgccg gtcctgccca tcctgatcgt attctccgcg
    17041 gtctacaaac ccagcatctg gaccatcatc gcggccctga tcctcttctt ctgggtgggc
    17101 cccgtgaaga ccgtccggtc catggcgctg cagatcaagg aggagaccta catcgaggcg
    17161 gccaaggccc tgggcgccgg gcacgggcgg atcatcttcc gccacatggc ccccctgctg
    17221 atcccctact ccttcgcgtc catggccctg gccgtgccgg gggccatcgt ctacgagtcc
    17281 tcggtgagcc tcctgggcct gggggacgcg accatcgtgt cctggggaca gatcctccac
    17341 gacgccctcg gctcgggcgc cgtgctctcg ggcctctggt ggtgggtggt cccgccggga
    17401 ctcctgatcg ccgtgatggg catgaccttc gcgttcctcg ggttcgcgat ggacaagatc
    17461 ctccatccga aactcaagac gaggtaggcc atgagcgaaa ccgtactaca ggtcaagaac
    17521 ctgaaactct actaccacac ctccaagggg gccgtgaagg ccctggacga cgtgtccttc
    17581 gacctgaaag ccggggaaac cctggggctc gtcggggaat cgggctgcgg caagacgacc
    17641 atgggcacgg gccttttgcg catgcccacc cctccgggac gcttcgaggg gggacagatc
    17701 ctggtggagg ggcgggacat cctgcccctg ccggagaagg agatccggaa ggatttccgg
    17761 tgggagaagg tctccatggt cttccagggg gccatgaact gcctgacccc ggtctacacg
    17821 atcggcaagc agatgatgga aaccctccag gagcaccggt ccatggaaaa agccgaggcc
    17881 caggagctca tcaaggaata ccttagctac gtggggcttc cggcggacgt gacgagccgg
    17941 tatccccacg aactctccgg cggcatgaag cagagggtcg tcatcgcgac ggcgctcttc
    18001 ctcaagcccc ggctggtcat cctggacgag cccaccacgg ccctggacgt gatagtccag
    18061 gcccagatca tcaacctcct caagaagctc aagaagacct tcgacctctc cttcatattc
    18121 atcacccacg acctggccct ggaggcggag gtctcggacc gcatctgcgt tatgtacgcg
    18181 ggaaagatcg cggagctggg gacgaacgag caggtctacg ggggcaaggg ccccgcccac
    18241 ccctacacgg aacggctcct ggccgcgacg ccccggctgc gccggaaggt ggagagcctg
    18301 tccttcatac ccggcacccc tcccgacctg atcgcccctc catcgggctg ccgtttccat
    18361 ccgcgctgcc ccagggtcta cgaacggtgc cggatggaag aacctcccgt ccgggagatc
    18421 gaaccgggcc atcttgccgc ctgctggagg tgcttcgaat gagcaacgga atcgacgggg
    18481 acgtcatcct gcgggtggac gaccttaaga aacacttcac cccccatcag ggcttcctcc
    18541 agtccttcgg caaggtccgg aaggtcgtcc gtgcggtgga cgggatctcc tttgagatcc
    18601 gccgggggga gatcttcggt ctgatcggag aatccggctc gggcaagacc acgacgggca
    18661 agctcgtcat gaagctgatc gacgccacga gcggccggat cctcttcaac ggccgggacg
    18721 tcacctccat cggacacgag gaaacgaagg agtaccggcg gcaggtccag atgatcttcc
    18781 aggatcccta cgcctccatg aatccccggt tcaagatccg ggacgtcctg gaggaacccc
    18841 tggagataca caggatcacc gcggaccgta aggaacgcga aacccggatc gtccgggcct
    18901 tgacggaggt caagctcacg cctcccgagg atttcatggg ccgcttcccc cacatgctct
    18961 ccgggggcca gcgccagcgg gtggccacgg ccaggaccct catcctgaac ccggccatgc
    19021 tggtcgcgga cgagccggtg tccatgatcg acctgtccac ccgggcggag atcctccaca
    19081 tgatgaagga cgtgcagagg gacctcggac tcacctacct ctacatcacc catgacctgt
    19141 ccacggcgcg gtacttcacg gaccgcatcg cggtcatgta cctgggacgc atcgtggaga
    19201 tgggcaaggc ggacgacatc atcgaccatc ccgcccatcc ctacacccgg gccctgatcg
    19261 aggcggttcc ggagcccgcc accggcaagg tggacgtcgt gaaggaactg ccgatttccg
    19321 gggaaatccc ctcgccgtcc aacatcccgg cgggctgccg cttccacacc cgatgcccct
    19381 acatgaagcc cgagtgcaag gaggaggccg agcccgaact catggacctc ggcagcggtc
    19441 attatcatgc gtgtcgcagg tggaaggaga tcggctgacg aattcctgaa gccagatccg
    19501 ggccgccggt tcgcgcctat gcgcgaaccg aaccgccggg cgccttcggg cgcccggcgc
    19561 tatttgggcg ggtcgcggag ggagaaggcc agcaccagca tcccgtaggt gacggccacg
    19621 aagaagctga aggtcaggga gaagagcacg gccttcggat ccgacagcgc cgccagggtg
    19681 ccccgcacgg cccgcacggg ggtgagcacc gcgtcccagg agttgagcct cacgaagcga
    19741 cccaggtaga ccccgaaccc cgcaaggatc accgccaccg taaggaagac tcgctcgaat
    19801 ccacggcccc aggcgtggtc cagcaccctt ccctccaccc acatggatac gagtcccagg
    19861 aaatgcccca cgaaggcgaa ggccccgctc atgacgatgt cgtaccagag gaccccccgg
    19921 gtcccgagcc attggatcgt atccgagcgt atccaggtct tctcggcgac gtggatgaag
    19981 tccgtgaaca ggtaaggcgc gttcgggaag aagagcagcc accccagggc gcagacggaa
    20041 accaggaccg tccgggccgg gccgggattc agggtccgga ggaggattac ctggacggct
    20101 gccatcccca gggggacgca ggcaaggagg aggttcatcc caaggaaggt gtagaagggg
    20161 ttgcccagaa gggcaactcg gactgccagg agggacaggc agcccgtgag cgccagggcg
    20221 gccaggaccc ccagggagag gacggagcgg aaggaaggga tggcgcccgg gcgggattcc
    20281 tcccggtcgc cttccttgag caggtcggtc aaggttccct tccccccgcc ccgctgtgtc
    20341 acagatccaa cgccttggcc cggaccttcc ggaggacccg gtccgtgaag tccgcgatct
    20401 tgagggcccc gagctgggtc ccgtcccgaa gacgcacggc caccgccccc tcctccgcct
    20461 ccttctgccc cacgaccagg atgtagggga tcttcgagtt ctgggcgttg cggatcttgg
    20521 cgttcatgcg gtcgtccgag aggtccgccc gggcacggag gcccgcggcc gcaagctccc
    20581 cggcgacctt cttggcatac tcgtcgaagg cgggggccac gggcacaacc accgcctgct
    20641 ccggggacag ccagatcggg aaggcccccg cggtgtgctc cagatagacc ccgaagaagc
    20701 gctcgacgga ccccaggagg gcccggtgca ccatgtaggg ccgcttgcgc tggccgtcgg
    20761 cgtccacgta ggtcatgtcg aagcgctcgg gctcgttgaa gtcgaactgg atggtggtca
    20821 gctgccactc gcgg
//
LOCUS       DAOTEI010000044        20749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1691_length_20749_cov_4.927660, whole genome shotgun sequence.
ACCESSION   DAOTEI010000044 DAOTEI010000000
VERSION     DAOTEI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 20749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20749
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_20749_cov_4.927660"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /gene="rpoB"
                     /locus_tag="P5313_06205"
     CDS             complement(<1..1937)
                     /gene="rpoB"
                     /locus_tag="P5313_06205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRY79989.1"
                     /translation="MAYTENKIKRSYIGKDYEEAIELPDLIGIQLSSYERFLQRERML
                     AGDKPLPAGLEEVFQATFPIESPNGDMLLSYEAYSLDESAMKYSEMECKQKGLTYAVP
                     LKARINLIFQKTGEIRQKEIYLGDIPLMTDRGTFIINGAERVVVSQIHRSPGVIFCHE
                     KGVYSARIIPYRGSWLEFEIDQKKELIYAKIDRKKRILGTMFLRALGFETREDILSAF
                     YGSRKIPLSESREDREGAVNKVLARSVWAEGSEGEKQKLYRAGDKLHLHEVDELVARG
                     IKEVEVIDFQAEDSLHFDMILNCLEREDIKLVKEDPTQDEPTKADALAAVYATLLPGE
                     PITVENAERDLHSMFFTTRRYDLGKVGRYKLNKKFDYDIPIQDFTLAKADIIATMKYL
                     MSVYYGESNIDDIDHLGNRRVRSVGELLANVMKSAFSRMERIAKERMSLKETDTIRPQ
                     DLVSIKPIVAAVKEFFGSSQLSQFMDQVNPLAELTHKRRLNALGPGGLSRDRAGFEVR
                     DVHYTHYGRMCPIETPEGPNIGLIVSLATYTTVNEYGFLETPYRKVKEGKVTKEIEYL
                     SAMDEDKYYIAQASAKIDGKGNFQEPSISCRHQGDYAMRGPKELQYMDVSPKQIISVS
                     ASLIPFLEHDDANRALMGSNM"
     gene            complement(2275..2664)
                     /gene="rplL"
                     /locus_tag="P5313_06210"
     CDS             complement(2275..2664)
                     /gene="rplL"
                     /locus_tag="P5313_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRY79990.1"
                     /translation="MAALSKDQILDAIANMTVLEVSELVKAMEEKFGVTAAAPVAVAA
                     AGAAGAAAAPAEEKTEFTVILKAGVPADKKIAIIKEVRAITGLGLKEAKDLVEAGDKA
                     LKENVSKDEAEKVKKQITDAGGAVEVK"
     gene            complement(2918..3448)
                     /gene="rplJ"
                     /locus_tag="P5313_06215"
     CDS             complement(2918..3448)
                     /gene="rplJ"
                     /locus_tag="P5313_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRY79991.1"
                     /translation="MAMRATKLQPSKTEAIGDLRKKIAESKDFLFADYRGLTVEQITG
                     LRKQLRAKNSYFHVVKNNFARIAFEEGGYPDVSTLLTGPTAVAFAKADSNEIAKILVD
                     FSKEAPVKLKGGLVDKAFYDKTMIEAFSRLPGKSQLISMLMSAMNGPVQNLVYVLNAV
                     PSKLVRTLKAVEESKR"
     gene            complement(3452..4135)
                     /gene="rplA"
                     /locus_tag="P5313_06220"
     CDS             complement(3452..4135)
                     /gene="rplA"
                     /locus_tag="P5313_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRY79992.1"
                     /translation="MKRGKNYDAAVKKVDRSKEYQASEACALVKEMKYAKFDETVEAH
                     VRLNLKKSQTVRDTVLFPNQFRAEKRIVVFCKPDKAKEALDAGALEAGDDALIEKVKG
                     GWLDFDVAVATPDMMKDVGKLGMVLGRRGLMPNPKTGTVTHDVAAAIAELRKGRTEFR
                     SDKSNIVHIAVGKVSMEPAKIGENLEILLAEIARKRPSDTKGDFIQSVYVASTMGPGV
                     RVAAGKSER"
     gene            complement(4140..4571)
                     /gene="rplK"
                     /locus_tag="P5313_06225"
     CDS             complement(4140..4571)
                     /gene="rplK"
                     /locus_tag="P5313_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRY79993.1"
                     /translation="MAKKKVTAIIKLQCPAGKATPAPPVGPALGPHGVSAPQFCQQFN
                     DRTKGMEAGLTIPVIITVYGDKTFTFITKTPPASVLIKKSIGLDKGSPVSHKQKVGKI
                     SKAKIEEIAKMKMADLSANDLAAASKIIAGTARSMGVEVEW"
     gene            complement(4910..5467)
                     /gene="nusG"
                     /locus_tag="P5313_06230"
     CDS             complement(4910..5467)
                     /gene="nusG"
                     /locus_tag="P5313_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRY79994.1"
                     /translation="MAKAWYVLHVYSGYENKIEKTIRVLLAGGELDPAVVTDIRVPSE
                     EVVDVKDGKKRTSSRKILPGYILVEMDLPDADWKGACSTIRKITGVTGFVGATQHHKP
                     APISQEEARHILQRSGEIKGERSAKTKQSFQAGEQVKIIEGPFESFTGTIEEVNSEKN
                     KLKVMVGIFGRSTPVEVDMLQVEKV"
     gene            complement(5480..5662)
                     /gene="secE"
                     /locus_tag="P5313_06235"
     CDS             complement(5480..5662)
                     /gene="secE"
                     /locus_tag="P5313_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRY79995.1"
                     /translation="MSKIVQFFKDSYAELRKVVWPTREDVLASTKVVIVSTVIVAFCL
                     GLLDFLLVRGIDFLFR"
     gene            complement(5718..5790)
                     /locus_tag="P5313_06240"
     tRNA            complement(5718..5790)
                     /locus_tag="P5313_06240"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5755..5757),aa:Trp,seq:cca)
     gene            complement(5870..6043)
                     /gene="rpmG"
                     /locus_tag="P5313_06245"
     CDS             complement(5870..6043)
                     /gene="rpmG"
                     /locus_tag="P5313_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRY79996.1"
                     /translation="MAAKKQTVEIIALACSECKRKNYTTKKNRKTTQEKLELMKYCRW
                     DRKYTLHKETKVK"
     gene            complement(6273..6345)
                     /locus_tag="P5313_06250"
     tRNA            complement(6273..6345)
                     /locus_tag="P5313_06250"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6310..6312),aa:Thr,seq:ggt)
     gene            complement(6428..8014)
                     /gene="murJ"
                     /locus_tag="P5313_06255"
     CDS             complement(6428..8014)
                     /gene="murJ"
                     /locus_tag="P5313_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRY79997.1"
                     /translation="MSDDAAPDSGSFVRESGRLSLLTLVSRVLGLVREMTRAAFMGTG
                     ALADAFTVSFLIPNFLRRLFAEGSIAVAFIPTFKGYLLEGNREDSEEFLCSMHTVLSL
                     LVGLTVLLGIALTPLIVLLFRSDPAETTILTRMMFPYLALVSFAAFLQGILNSVHVFV
                     PAGVAPILFNLGFIAVPYALAPFLPNPARALAVGVLAGGLAQALCQLPAVLRRGYRFR
                     FIPPARAFRNPGMRRVLALIAPTILGMAAYQVNELVCTAVASGAGVGVATSLTFSLRL
                     QELVLGIFAVSIGTVMLPDLSERAKLGEWEAFSDRLEGALKAIVLVTIPVAVFSMIEG
                     RDIIVLLFKAREFGEESVNLTVRAFFFHMTGLVFIAANRILAPAFYAQEDTKSPVRAG
                     LASFAVNIVLAVVLAGPMGGGGIALALSLGSALNTVILVRLLIRRRIPGMSRAAASVA
                     RYALRILAFSAPAGAAAYLCRRVLEGPLADARGRFLAAGLPLAVCTLAFAAVGVGLLA
                     ATRDEAAAGLVRALRRSRGR"
     gene            8098..8913
                     /locus_tag="P5313_06260"
     CDS             8098..8913
                     /locus_tag="P5313_06260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRY79998.1"
                     /translation="MDIRLVALDLDDSLLRQDLSISEANAEACRKAMGQGVRVVLASG
                     RTERAMLKYARMLGAEGPGEYLISYNGAKILDLGSGEHLYERRIPPETCRRTAALLSE
                     RGFPYQFYMDEGRIVTTVRNRWTEEDSRLTGLPIEVMEDPGPYLDRGQLKFVVAGEPG
                     ALQVLRGELSALLEGRAEVLISKPYFLEVLSAGTDKGTALEVLADRLGIPLESVLAVG
                     DAQNDLGMVRTAGWGCAPSNAIPAVKAAARYVSPRSHEEDAVAEILERFVLGT"
     gene            9144..9332
                     /locus_tag="P5313_06265"
     CDS             9144..9332
                     /locus_tag="P5313_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY79999.1"
                     /translation="MSQDLKPIAARRCYACIQWEGVRSYDRGTQMVKVDVGREGKCLV
                     HRTMVKGSHFCDQFFQLK"
     gene            9436..10026
                     /locus_tag="P5313_06270"
     CDS             9436..10026
                     /locus_tag="P5313_06270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRY80000.1"
                     /translation="MEPCRIRRALPADLGDILSIEDDSFDPAIRESPDTFRERMEVFP
                     AGFVILETPEGSAGYLCSELWDLSGPPTPSDFALGHSARDRHSPRGRTLYVSSYGIRK
                     AFRGRGLGRALFRDFLERAPADFPFDEILLLVSRTWSEARRIYEAEGFRQVLTVPGFF
                     RFSDGSSADGEVLRRPRVRDSTGTPSAPMMKDKELP"
     gene            10023..12323
                     /locus_tag="P5313_06275"
     CDS             10023..12323
                     /locus_tag="P5313_06275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY80001.1"
                     /translation="MRRSALALVSALAAAAAAFGDIRFSGPDLSQKDELLFSAEATLP
                     GGGAFRTLFSADVAAGKLRQLTFYPERVTLVDRGRRLQVQNRYGVFRTGADFRGMEPV
                     PGFPAFTRGSPAREGKLPGVQASPDGTRLLYMDPASPAYGRLILFDVATGGETVVSAD
                     VAQYADRIPALWSPDSRHFVYAKGGNLHYYSLDQHEGKRVLDESFRRVGPGRIENAQW
                     NEQGSLYILRHRSLYRILPAEFFAQALYAGVVSIGTMVGKVPFPFDPNFDSYRVSPDG
                     RKILLCKDGRNLFLYYLDPDDFGKADRVAALPYLFLQGNTTVTEVLWPESAELTILTS
                     SLRDGERVSGAYRIAAPDSPADLSLTQSFKTLDASGAVSVELSPDRKRVALANDRGVV
                     IRRYADWSAVTTLDAPGTLHALWASDERLVLAGSRTIELAAADGSERVLLALSQADRY
                     GWAPETPGTVAALAGNSAWTLEPGSKDWKRAAAFKTLPASGASPAYRVYLDELDSGSY
                     RNIVMVRSIQGFGTKPLLLPAPRPWAPFPERDEPRSPGIFDHGSRIRRRQVALVFNAY
                     DSSEGLTGILNTLADYRIRATFFVNGEFVRRNPGAARLIAESGHETGNMFFTTYDPTD
                     ARFRVDAEFVKRGLARTEDEYFAATGRDLSLLWHTPHYSTSTEILEAASRMNYTFVGR
                     DVDPLDWVSETEGKSVPGLYLDAHRIVERVLAKVKPGSIVPVRVGVPEGGRQDYLFRE
                     LSLLVNGLLAEGYEIVPVSTLIEHAR"
     gene            12320..14728
                     /locus_tag="P5313_06280"
     CDS             12320..14728
                     /locus_tag="P5313_06280"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY80002.1"
                     /translation="MNAESLRLSPGDIAFSVTEQDILRLEGGDWGKAVIGQPRALEAL
                     LMGTGIRAKGYNIYISGAPGTGRRTAVMQVLSEVAANPDTLRDRAYAYNFRSPLEPRA
                     LSFPRGQARAFKKDLHALVENMKKLVRLQTESADFKGRLEAATREVETEETRRLSEFE
                     AELAAQGFRIAQAQDEEGSTTTDIIPLRNGEPSSFDALQAEVASGGLAEEEWNRLRGL
                     YHESMDRLKSLFAELRRGRVELERRLENLRHEALRPFVLAETELLRKRYPDPRIHAWV
                     SDLERDLLQHLRLFLADPEPEPPLPARRPVPPLARYGLNILVDNGQAERPPVILENHP
                     SSVNLFGFVEPRPDERGDTRAAYLKIRPGAFHRADGGYLILRAEDLVQDEEAWLWMKR
                     VLQTGAVEIQPSAGPFGSPGAPTKPQAAEVDVKVILLGGEMTYDALYAADPDFQKLFK
                     VYAEFDSTMPRNDETMREYVAFIRKIVREEGLLPADPDAIAAVVEYGVRLSEFRNRLS
                     TRFSLIADLLREASWHAGRAGRTAMDAGSVTTAISARAYLANLPEEKVEEMIASGQII
                     LQVSGSAVGRVNGLAVHDRGYYAFGLPAVISAQVSPGESGVINIEGESGLSGEIYDKA
                     VLIVEGFLRSRYARGFPLMVTASICFEQSYTAIEGDSASSTAVYALLSAIAGVPLRQD
                     IAVTGSVNQMGQIQPVGGITEKIEGFYQVCRRAGFTGTQGVLIPRQNAVNLTLSRPVQ
                     EALREGRFHIWAVSTIDEGVAVLTGMSAGSPNEKGEFPPDTFNARVRKELQRMARTIK
                     SYLG"
     gene            14815..16338
                     /locus_tag="P5313_06285"
     CDS             14815..16338
                     /locus_tag="P5313_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRY80003.1"
                     /translation="MAANELVVDVSKIKKAVHSAIPLTITTYTLPHEIEIYLEEVLDV
                     FLGELGQKKLKDYLVYCLRELAVNAKKANTKRVYFDSRGLSINDPSDYEEGMKSFKAD
                     TLENIAWYLAKQKEKGYYIKIVLQAKGSTVVLEVRNNVEINRTEYVRIHDKLARSRKY
                     TSLEEALQQVLDPSEGAGLGLVILVLMLKKIGLDEECFDIRAESGETLARITVPVEAT
                     RIESLSKLTDEIVRQITSLPHFPDNIVAVQKLLADPKSEMVDIARHISTDPAMTADLL
                     KLVNSAQFMLPKRVDNIVDAVKLVGLKGIRNQLYSYGAQKLLGDDTDTKKKLWDHSYR
                     TAYYAYNLVKNFKPAKRHILDDAYVGGMLHDMGKIVFSTVHPDLLDRIRAFCGEKDIP
                     VQTFEDIAGGMNHAEIGARIAEKWNFPESLVASIRFHHEPGLMTMDYKDVVFAVYLAN
                     AFCEIENGGMTWDQLEPLVIQDYGFQGEAQFRKIVEQFSEGFNKETRRLAGEAGKKA"
     gene            16508..17524
                     /locus_tag="P5313_06290"
     CDS             16508..17524
                     /locus_tag="P5313_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRY80004.1"
                     /translation="MNTPPDPAGPGSDGDARSGGLAEAAFRLNALLEAAMPVITPTGV
                     VLGFLFPDFFVRLRPYIPWLFACITLAGALKLRARDLGAAAASPIPVLDFFAAAHVLM
                     PVLAWAVSRLVLPGDTEAAAGFVLLFSAPTAVAGFIWVSIYRGDEALALALILLDTLG
                     APIVMPFTVSALLGTGIRLDGTAMAASLALMVVLPTVLGVALNEASRGRIPRAVRPYL
                     TPLSKILLALVIAANSAAVAPSVDFSEARVWAVGAAGIGLTTAAFFLGKTLGLVPGIG
                     PARRRGLVFSVGLRNISAVATIAITFFPEAAALPAVLGMFFQQTLAAVFGRILLGKPS
                     KSRE"
     gene            complement(17507..18268)
                     /locus_tag="P5313_06295"
     CDS             complement(17507..18268)
                     /locus_tag="P5313_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRY80005.1"
                     /translation="MRYALLTTACILLAACMPPAPKSPPSDPLAPYRAALAGLPPEIM
                     ARAEAAPVRFAGLLRRIWAVEDPLYLARVDKEKPLPADYEPGDLVSLAEYPFALARRD
                     LRLRKPAAEAARELDAAAKAAGVTLVFSSAYRSYAYQSEVYERHVRELGEAAADRISA
                     RPGRSQHQLGTVVDFGSIDNTFAESKAGLWMAANAGRFGFSLSYPRGAEGLTGYDWES
                     WHYRYVGKEAAALEAEFFGLQHWMLVFLERLFSGF"
     gene            18356..20287
                     /locus_tag="P5313_06300"
     CDS             18356..20287
                     /locus_tag="P5313_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80006.1"
                     /translation="MPSSFRPAPRRVLSAALRLALAASALALAASCGSGGSGRYTVPG
                     LEGVGPESRLVRQAREEYVDLPGVRGPEAASPKPGEVPADWRTYGEGSPSRLAVLLTD
                     PRSSWLGLVHGFKSLGIPFRVTDSLDSALDHDVVLAYPGFKRGSLDDTEARRLEDFVR
                     GGGTLLAVQTGRPEPRVEALFGFRGAVRNRRRSELRYEDSPFAAGLEYPEERTTRLGN
                     PDRARYRLRTAAFSDPEEVLARYEDGGAAVIRRRDGAGTAMAFGFDLGRYIQGAQGNR
                     DDEANRTYVNSFEPSVDSLLRFLGEVYRAREPDAVFLHPAPDGMPLSVVVSHDVDYAG
                     SLANSVEYAEYERSMGYGATYFLQTKYFRDFFDEVFFDERTPELLARLEELGMELASH
                     SVCHSDMFARVPQGTFRESYPDYQPRIIEFYYTRNATIMGELRVSKFLIERLSRSRVD
                     SFRPGFLANPFSLPQSLESAGYRYSSAVAANDVMTHLPFRLNHDRLYRSETAVYEFPI
                     TVEDEKDPPMDGRVEDAVELAGKISRYGGLFVILIHPNVTAEKLRFLRAFSERMREKA
                     WWGTLREFGDWWTARDGVESDVVPSGDRRVLRLSSPLPVRGLGIRLPEGFRIAAPRGA
                     NGTFVLDLPAGTTELVLEP"
     gene            complement(20262..>20749)
                     /locus_tag="P5313_06305"
     CDS             complement(20262..>20749)
                     /locus_tag="P5313_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY80007.1"
                     /translation="GGLYKQRDRWHRGLVEIMTWHRKMLFNPKYGSAGLLAFPYFLAF
                     EVIGPFYEFAGYPLMILGFAVGALSLPVFLVMFSAVLLFGLLISLVSLALSERDIVYF
                     RPVELARLLGYSILENFGFRQLMGWVRVFSVLQLLTRNKGWQKLERKGFLPSRVPGPA
                     P"
BASE COUNT         3448 a         7017 c         6771 g         3513 t
ORIGIN      
        1 tgcatgttcg agcccatgag ggcgcggttg gcgtcgtcgt gctcgaggaa ggggatgagc
       61 gaggccgaga ccgagatgat ctgcttgggg gagacgtcca tgtactggag ttccttggga
      121 ccccgcatgg cgtagtcgcc ctggtggcgg caggagatcg agggctcctg gaagttcccc
      181 ttgccgtcga tcttggcgct ggcctgggcg atgtagtact tgtcctcgtc catggcggac
      241 aggtactcga tctccttggt gaccttgccc tccttgacct tgcggtaggg ggtctccagg
      301 aagccatact cattgaccgt ggtgtaggtg gccagggaga cgatgagtcc gatgttcggt
      361 ccttccgggg tctcgatggg gcacatccgg ccgtagtggg tatagtggac gtcccggacc
      421 tcgaagcccg cccggtcccg ggaaaggccg ccggggccca gggcgttcag gcgccgcttg
      481 tgggtcagct ccgccagggg gttgacctgg tccatgaact gggagagctg gctggagccg
      541 aagaactcct tgaccgcggc cacgatcggc ttgatggaca cgaggtcctg gggccggatc
      601 gtgtccgtct ccttgaggct catcctctcc ttggcgatcc gctccatgcg gctgaaggcg
      661 ctcttcatca cgttggccag gagctcgccg acggaccgga cccgccggtt gcccaggtgg
      721 tcgatgtcgt cgatgttgct ctcgccgtag tagacgctca tcaggtactt catggtggcg
      781 atgatgtccg ccttggccag ggtgaagtcc tgtatgggga tgtcgtagtc gaatttcttg
      841 ttgagcttgt agcggcccac cttcccgagg tcgtagcggc gggtggtgaa gaacatggag
      901 tggaggtccc gctcggcgtt ctccacggtg atgggctccc cgggcaggag ggtggcgtag
      961 accgcggcca gggcgtccgc cttggtgggc tcgtcctggg tcgggtcctc cttgaccaac
     1021 ttgatgtcct cccgctccag gcagttcagg atcatgtcga agtggaggga atcctcggcc
     1081 tggaagtcga tgacctcgac ctccttgatc ccccgggcca ccagctcgtc cacctcgtgg
     1141 aggtggagct tgtcgccggc ccggtagagc ttctgcttct cgccttcgga gccctcggcc
     1201 cagacgctcc gggccaggac cttgttgacc gccccttccc ggtcctcccg ggactcggac
     1261 agggggatct tccgggagcc gtagaaggcg gacaggatgt cctcccgggt ctcgaagccc
     1321 agggcgcgga ggaacatggt gcccaggatg cgcttcttcc ggtcgatctt ggcgtagatg
     1381 agctctttct tctgatcgat ctcgaattcg agccagctgc cccggtaggg gatgatccgg
     1441 gcggaataga cgcccttctc gtggcagaag atgacccccg gggagcggtg gatctgggag
     1501 accaccaccc gctcggctcc gttgatgatg aaggtccccc ggtccgtcat gagggggatg
     1561 tcgcccaggt agatctcctt ctggcggatc tcgcccgtct tctggaagat gaggttgatc
     1621 cgggccttca ggggaacggc gtaggtcaag cccttctgct tgcactccat ctcggagtac
     1681 ttcatagcgc tttcgtccag ggaataggcc tcgtaggaca ggagcatgtc gccgttaggg
     1741 ctctcgatgg ggaaggtggc ctggaagacc tcctcgagcc ccgcgggcag aggcttgtcc
     1801 cccgcgagca tgcgctcccg ctggaggaac ctctcgtagc tggaaagctg tatgccgatc
     1861 aggtccggga gctcgatggc ctcctcgtaa tccttgccga tgtacgatct ctttatcttg
     1921 ttctcggtat acgccatccg tacccccctc gtgagagctg ctgccagagc gcgccattct
     1981 tacattcccg gggcgggaaa cccccgcggg cttcccccaa atccccggcc ggccgatacc
     2041 ttgcaggctg accgcaaacc ctcgtacggg cttgccggca tcctgcacgg ccctggaggc
     2101 ggatgccccc agggagccgc cgccttccgg tagaccggga ccggcggctc gccgccgatc
     2161 ctcgatccgc cttccagcgc gaaagggttc cgggcggcct acgcccggaa cccgtccccc
     2221 gtggcctggg acggccgcgg gggcagaggc tgactcgtct cgtccgccaa gagcttactt
     2281 cacctcgacg gcgccgccgg catcggtgat ctgcttcttg accttctcgg cctcgtcctt
     2341 ggaaacgttc tccttgaggg ccttgtcccc ggcttccacc aggtccttgg cctccttgag
     2401 gcccaggccg gtgatggcgc ggacttcctt gatgatcgcg atcttcttgt cggcggggac
     2461 gccggccttg aggatgacgg tgaactcggt cttctcctcc gcgggagcgg cggcggcgcc
     2521 ggcggcgccg gcggcggcca cggcgacggg ggcggcggcg gtcacgccga acttctcctc
     2581 catggccttg acgagctcgg aaacctcgag aacggtcatg ttcgcgatgg cgtcgagaat
     2641 ctgatccttg ctgagcgctg ccatattaac ttctccttcg gacaggtttc atcccccacg
     2701 ggggacgccg gccgggggcc ggaactcagg cggacaggac gacagcgacg aagcctgaac
     2761 gccgtatcgg tgaacggtgg acggtggacg gtggaccgaa tgccaccgta ctaccgccac
     2821 cctcacgtcc caacaattgc cggcgcggcc ccttcggccc gcgccctttt taaaaatcga
     2881 cgaacgtcag ttcgtcgcgc caagcgctga cccaaggtca gcgcttggac tcctccacgg
     2941 ccttcagcgt ccgaaccagt ttgctgggca cggcgttgag gacgtagacc aggttctgca
     3001 cgggcccgtt catggcggac atgagcatgg agatgagctg gctcttgccc ggcagacggg
     3061 agaaggcctc gatcatggtc ttgtcgtaga aggccttgtc cacgaggccg cccttgagct
     3121 tgaccggcgc ctccttggag aagtccacca ggatcttcgc gatctcgttg gaatccgcct
     3181 tggcgaaggc gacggccgtc ggccccgtga gcagggtgct gacgtccggg tatccgccct
     3241 cctcgaaggc gatccgggcg aagttgttct tcacgacgtg gaaataggaa ttcttcgccc
     3301 gcagctgctt ccgcaggccc gtgatctgct cgaccgtgag cccgcggtag tcggcgaaca
     3361 ggaagtcctt gctctcggcg atcttcttcc tgaggtcgcc gatggcctcc gtcttggaag
     3421 gctgcagttt cgtagctcgc attgccatgg cttacctctc gctcttgccg gcggcgaccc
     3481 gcacgccggg ccccatggtg gaggcgacgt atacggactg gatgaagtcg cccttggtgt
     3541 cgcttggccg cttacgggcg atctcggcca gcaggatctc caggttctcc ccgatcttgg
     3601 cgggctccat ggagaccttg cccaccgcga tgtggacgat gttggacttg tcgctacgga
     3661 actcggtccg gcccttgcgc agttcggcga tggccgcggc cacgtcgtgg gtcaccgtgc
     3721 cggtcttggg gttcggcatg aggccccggc ggcccaggac catgcccagc ttgccgacgt
     3781 ccttcatcat gtccggcgtg gcgacggcca cgtcgaagtc gagccagccg cccttcacct
     3841 tctcgatgag ggcgtcgtcc ccggcctcca gggcgccggc gtccagggct tccttggcct
     3901 tgtcgggctt gcagaacacc acgatccgct tttccgcccg gaactggttg gggaagagga
     3961 ccgtgtcccg gaccgtctgg ctcttcttga ggttgagccg gacgtgggct tccacggtct
     4021 cgtcgaactt ggcgtacttc atttccttga caagggcgca ggcctcggag gcctggtatt
     4081 ccttggagcg gtccactttc ttgaccgccg cgtcgtagtt ctttccgcgc ttcatcggtc
     4141 taccactcca cttccacgcc catgctgcgg gccgttccgg ctatgatctt ggatgccgcc
     4201 gccaggtcgt tggcggacag gtccgccatc ttcatcttgg cgatttcctc gatcttggcc
     4261 ttggaaatct ttcccacctt ctgcttgtgg gagaccggag aacccttgtc caggccgatc
     4321 gacttcttga tcagcaccga cgccgggggc gtcttggtga tgaaggtgaa ggtcttgtcg
     4381 ccgtagaccg tgatgatgac cggaatcgtg agaccggcct ccatcccctt ggtcctgtcg
     4441 ttgaactgct gacagaactg gggagcggag accccgtggg gaccgagggc aggtcctacg
     4501 gggggagccg gcgtcgcctt gccggccggg cactgcaact tgatgatcgc agttaccttc
     4561 tttttcgcca tcttctgctc ctttcgtggt gtactcgacc ggtccaagcc ggccgagata
     4621 gcgtccgtcg cccgagggct gcggactccc atctgtcgga gattaacccg cgctatgaga
     4681 agcgccgccg cgttccggaa agtccgaagc gcgccgccgc cgccttgcaa agaaccctaa
     4741 gccccgcggc cggatgacct tccggggccg atcgcctaac agggtgttga aaaaggctat
     4801 agagcctttt tcaacacttc ctcaatatcg caattgcgca gagaaccgta aggttctcga
     4861 gcaattgcat agcaggttgt tgaaatgcct caagcatttc aacaacctgc tagaccttct
     4921 ccacctgcaa catgtcgact tccacggggg tggatctccc gaagatgccg accatgacct
     4981 tgagcttgtt cttctccgaa ttgacctctt cgatggtgcc cgtgaaggat tcgaagggcc
     5041 cctcgatgat cttgacctgc tccccggcct ggaaggactg cttggtcttg gcgctccgtt
     5101 cgcccttgat ctccccggaa cgctggagga tgtgccgggc ctcctcctgg gagatgggag
     5161 ccggcttgtg gtgctgggtg gcgcccacga agcccgtgac gcccgtgatc ttgcggatgg
     5221 tggagcaggc ccccttccag tccgcgtcgg gcaggtccat ctccaccagg atgtagcccg
     5281 ggaggatctt ccgggaggag gtgcgcttct tgccgtcctt gacgtccacg acctcctcgc
     5341 tggggacccg gatgtccgtc acgaccgcgg gatccagctc gccgccggcc aggagaacgc
     5401 gtatggtctt ctcgatcttg ttctcgtatc ccgaatagac gtggagaacg taccaggcct
     5461 tcgccatcct cgccgcgcct taccgaaaga ggaagtcgat gccccggacc aggaggaaat
     5521 ccaggaggcc caggcagaag gcgacgatca ccgtggagac gatcacgacc ttggtggagg
     5581 ccaggacgtc ttcccgggtc ggccaaacga ccttccgaag ctccgcgtag ctatccttga
     5641 aaaactggac gatcttgctc atgccggctc cttgagcgga aacgccccgg aggacggaac
     5701 cgcacggaat ggaaaaacag gccaggtagg actcgaaccc acaacatccg gttttggaga
     5761 ccggcgctct accattagag ctactggcct tcgtccgcga gcggaactcc ggaagggaat
     5821 tccgtccgcg gacgacgcga ttccgggtga gcccggaatc acggaacgct tatttgacct
     5881 tggtttcctt gtgcagggtg tacttccggt cccagcggca gtacttcatg agctcgagct
     5941 tttcctgggt ggtcttccgg ttcttcttcg tggtgtaatt cttccgcttg cactcgctgc
     6001 aggcaagggc gatgatctcc acggtctgtt tcttggcggc catgacgcta tccttctatc
     6061 caagattctc cgccgtggat gccccgaggg aaatcgcacg gcccgaaacg cgttccagaa
     6121 ccggcctgcg ccggaataca gcccccgatc ggtgcgccgc gggcgccccg acttgaaagc
     6181 tggttcaacc tgcggttgaa cgcatagccc ccgatcggat cgcccaaagg gcgaaccgac
     6241 ttgaagcctt ggtctagcat cgctagacgc atagcccccg atcggaatcg aaccgatgac
     6301 ctcatcctta ccatggatgt gctctgccga ctgagctaca agggcatagg tagtgccgga
     6361 atgataatga ccgcccaccc ctttgtcaat acatggcggg gggaagtttc acttggaacg
     6421 gaacttgcta acggccgcgg ctgcgccgga gagcccggac caagcccgcc gcggcctcgt
     6481 cccgggtcgc cgcgagaaga cccaccccga cggccgcgaa ggccagggtg cagaccgcta
     6541 ggggcagtcc cgcggccagg aaacggcccc gggcgtccgc gagggggccc tccaggaccc
     6601 tgcggcacag gtaggccgcg gccccggcgg gggcggagaa ggccaggatg cgcagggcat
     6661 agcgggccac ggacgcggcg gcccgggaca tcccggggat ccggcggcgg atgagcagcc
     6721 ggaccaggat cacggtgttc agggcgctgc ccagggagag ggccagtgcg atcccccctc
     6781 cccccatcgg cccggccagg acgaccgcga ggacgatatt cacggcgaag gacgccagcc
     6841 ccgcccggac ggggcttttc gtgtcctcct gggcatagaa ggcgggggcc aggatccggt
     6901 tcgcggcgat gaagaccagg cccgtcatat ggaagaagaa ggcgcggacc gtcaggttga
     6961 cggactcctc cccgaactcg cgggccttga acagaaggac gatgatgtcc cgcccctcga
     7021 tcatcgagaa cacggccacg gggatcgtga ccaggacgat ggccttcagg gccccctcca
     7081 ggcggtccga aaaggcttcc cactccccca gcttggcccg ctccgagagg tccggaagca
     7141 tgaccgtgcc gatggagacc gcgaagatcc ccaggaccag ctcctgcagt cgcagggaaa
     7201 aggtcagact ggtggccacc ccgaccccgg ccccggaggc cacggcggtg cagaccagct
     7261 cgttcacctg gtaggcggcc atgcccagga tcgtcggggc gatcagggcc aggaccctgc
     7321 gcatccccgg gttgcggaag gcccgggcgg gggggatgaa ccggaagcgg tagccccggc
     7381 gcaggaccgc ggggagctga cagagggcct gggccaggcc cccggccagg acgccgaccg
     7441 ccagggcccg ggcggggttg ggaaggaagg gggccaaggc gtagggcacg gcgatgaagc
     7501 ccaggttgaa caggatgggc gcgacccccg cgggcacgaa gacgtggacc gagttcagga
     7561 tgccctgcag gaaggcggcg aaggaaacga gggccaggta ggggaacatc atccgggtca
     7621 ggatggtcgt ttccgcgggg tccgaccgga acagcaggac gatgaggggg gtgagggcga
     7681 tccccaggag gaccgtcagg cccaccagga ggctgagcac cgtgtgcatg gaacagagga
     7741 attcttcgct atcctcccgg tttccctcca ggaggtagcc cttgaaggtg gggatgaagg
     7801 ccacggcgat cgacccctcc gcgaagagac gccgcaggaa gttggggatc aggaaggaca
     7861 cggtgaaggc gtccgccagg gcgcccgtgc ccatgaaggc cgcccgggtc atctcccgca
     7921 ccaggcccag gacccgggag acgagggtca ggagggagag ccggccgctc tcccgcacga
     7981 aggaccccga atccggggcg gcgtcgtcgg acatgggcac gaggataccg gaaggagggt
     8041 cccgggacaa gcccggggcc ttgagccgtc ccgccggctt cgggtatacc cttgcccatg
     8101 gacatacggc tggtcgcgct ggacctggac gattccctgc tgcggcagga cctgagcata
     8161 tcggaggcga acgccgaggc ctgccggaag gccatgggac aaggcgtccg cgtagtcctg
     8221 gcctccggcc ggaccgagcg ggcgatgctg aagtacgccc gaatgctggg cgccgagggc
     8281 cccggggagt acctgatctc ctacaacggg gccaagatcc tggacctggg ctccggcgag
     8341 cacctctacg agcgccgcat cccgccggag acctgccgcc gcaccgcagc cctcctttcg
     8401 gagcggggat tcccctacca gttctacatg gacgagggcc gcatcgtcac caccgtccgc
     8461 aaccgctgga ccgaggagga ttcccggctc acgggcctgc ccatcgaggt catggaggat
     8521 cccggcccct acctggaccg ggggcagctg aaattcgtgg tggccgggga acccggggcc
     8581 ctccaggtcc tgcgcgggga actctccgcc ctcctggaag ggcgggcgga ggtcctgatc
     8641 tccaagccct acttcctgga ggtcctgtcc gcgggcacgg acaagggcac ggccctcgaa
     8701 gtcctggcgg atcggctggg aatccccctg gagtccgtcc tggccgtggg ggacgcccag
     8761 aacgacctgg ggatggtgcg gacggcgggc tggggctgcg ccccttcgaa cgccatccct
     8821 gcggtcaagg cggcggcccg gtacgtctct ccccggtccc acgaggagga cgcggtggcc
     8881 gagatcctgg aaagattcgt cctgggaacc taggaacctg tcagacttgt ggttttttag
     8941 cgccgacagg cgccaaaaaa cccgattatg cagctccttt ggagtttgca aggtagaaaa
     9001 atcgcacaaa tgcgattttt ctactattta gctgggcaag cccgaaaaac tcctagggcc
     9061 ccgcgcggtc cgggcgccct caggtttccc gatcaaaact acgatattat ggaagtatag
     9121 aaaatgacga accggaggag accatgtccc aggacttgaa gcccatcgcg gcaagacgct
     9181 gctatgcctg catccagtgg gaaggggtcc gcagctacga ccgcggcacc cagatggtga
     9241 aggtggacgt cgggcgggaa gggaagtgcc tcgtccaccg cacgatggtc aagggcagcc
     9301 acttctgcga ccagttcttc cagctgaaat agacctcccg cggtcctcgg ggtcgtcgcg
     9361 cccggcaccc cccgggctcg agcccgaggt tgatcccggc ttggccatcc cgttccggac
     9421 ggctagaatg ggaccatgga accctgcagg atccgccgcg cccttcccgc cgacctgggc
     9481 gacatccttt ccatcgagga cgattccttc gatcccgcca tccgcgagag cccggatacc
     9541 ttccgggagc ggatggaggt ctttccggcg ggcttcgtga tcctggaaac cccggaagga
     9601 agcgcgggct atctgtgttc cgagctctgg gacctgtccg gccccccgac gccctccgac
     9661 ttcgccctgg gccattccgc ccgggaccgg cattcccccc ggggacgcac cctgtacgtg
     9721 tcctcctacg ggatccggaa ggccttccgg ggccggggcc tgggccgtgc cctgttccgg
     9781 gatttcctgg agcgggcccc ggcggacttc cccttcgacg agatcctgct cctggtcagc
     9841 cggacctggt ccgaggcccg gaggatctac gaggccgagg gattccggca ggtcctgacc
     9901 gtcccgggct tcttccgctt ctccgacggt tcctccgccg acggggaggt cctgcggcgg
     9961 ccccgagtcc gggactcaac tggcacgccc tccgcgccga tgatgaaaga caaggaatta
    10021 ccatgagacg aagcgcccta gccctagtgt cggccctggc cgcggccgcg gccgccttcg
    10081 gggacatacg gttctccggt ccggaccttt cccagaagga cgagctcctc ttctccgccg
    10141 aggcgaccct gcccgggggc ggcgcgttcc ggaccctgtt ctccgcggac gtggccgccg
    10201 ggaagctgcg ccagctgacc ttctacccgg agcgggtgac cctggtggac cggggtcgcc
    10261 gcctccaggt ccagaaccgg tacggagtct tccggacggg ggccgacttc cggggcatgg
    10321 agcctgtccc gggcttcccg gccttcacgc ggggcagtcc cgcccgggag ggcaagcttc
    10381 ccggggtcca ggcctccccg gacggcaccc gcctcctgta catggatccc gcctcgccgg
    10441 cctacggccg gctcatcctc ttcgacgtgg cgaccggcgg ggaaaccgtg gtctccgcgg
    10501 acgtggccca gtacgcggac cggatcccgg ccctgtggtc cccggactcc cggcacttcg
    10561 tctacgccaa gggcggcaac ctccactact actccctgga ccagcacgaa ggcaaacggg
    10621 tcctggacga gtccttccgc cgagtcggcc cgggccggat cgagaacgcc cagtggaacg
    10681 agcagggcag cctgtacatc ctccgccacc ggagcctcta ccgcatcctt cccgcggagt
    10741 tcttcgccca ggccctctac gcgggggtgg tctccatcgg gaccatggtg gggaaggtcc
    10801 ccttcccctt cgatcccaac ttcgactcct accgggtatc cccggacggc cggaagatcc
    10861 tgctgtgcaa ggacgggagg aacctcttcc tctattacct ggacccggac gacttcggca
    10921 aggccgaccg ggtggccgcc ctcccctacc tcttcctcca gggcaacacc acggtcaccg
    10981 aggtcctctg gccggagagc gcggagctca ccatcctgac ctcctccctc cgggacggcg
    11041 agcgggtgtc gggggcctac cggatcgcgg ccccggactc cccggcggat ctttccctga
    11101 cccagagctt caagaccctg gacgcctcgg gcgcggtttc cgtggagctt tccccggacc
    11161 gcaagcgggt ggccctggcc aacgaccgtg gggtcgtgat ccggcgctac gcggactggt
    11221 ccgcggtcac caccctggac gccccgggca ccctccacgc cctctgggcc tcggacgaac
    11281 gcctcgtgct ggcgggatcg cggaccatcg agttggccgc ggcggacggc tcggagcgcg
    11341 tccttctggc cctgtcccag gcggaccgct acggctgggc ccccgagacc ccgggcaccg
    11401 tggcggccct ggccggcaac tccgcctgga ccctggagcc cggctccaag gactggaaga
    11461 gggcggccgc cttcaagacc ctccccgcct cgggggcttc cccggcctac cgggtctacc
    11521 tggacgagct ggactcggga tcctaccgga acatcgtcat ggtccggtcc atccagggct
    11581 tcgggaccaa gcccctcctc ctgcccgccc cccggccctg ggcccccttc ccggaaaggg
    11641 acgagccccg gtccccgggg atcttcgacc acggatcccg gatccgccgc cgtcaggtgg
    11701 ccctggtctt caacgcctac gactcctcgg agggcctcac gggtatcctg aacaccctgg
    11761 cggactacag gatccgggcc accttcttcg tgaacgggga gttcgtacgc cgcaatcccg
    11821 gggccgcccg gctgatagcc gagagcggac acgagacggg gaacatgttc ttcaccacct
    11881 acgaccccac ggacgcccgg ttccgggtgg acgcggaatt cgtcaagcgc gggctggccc
    11941 ggacggagga cgagtacttc gccgccaccg ggcgggacct ctccctgctc tggcacaccc
    12001 cccactactc caccagcacg gagatcctgg aggccgcgtc ccggatgaac tacaccttcg
    12061 tgggacgcga cgtggatccc ctggactggg tcagcgagac ggaggggaag tccgtgcccg
    12121 ggctctacct ggacgcccac cggatcgtgg agcgggtcct ggccaaggtc aaaccgggtt
    12181 ccatcgtgcc cgtccgcgtc ggggtgcccg agggagggcg gcaggactac ctcttccggg
    12241 agctgtccct gctcgtgaac gggcttctgg ccgaggggta cgagatcgtg cccgtatcca
    12301 ccctgatcga gcacgcccgg tgaacgccga gagcctccgg ctgtcccccg gggacatagc
    12361 cttttccgtg acggagcagg acatcctccg tctggaggga ggggactggg gcaaggccgt
    12421 catcggccag ccccgggccc tggaggccct gctgatgggc acggggatcc gggccaaggg
    12481 ctacaacatc tacatctccg gcgcccccgg tacgggccgg cgcaccgcgg tcatgcaggt
    12541 cctctccgag gtcgccgcga atcccgacac cctccgggac cgggcctacg cctacaattt
    12601 ccgctccccc ctggagcccc gggccctctc cttcccccgg ggccaggccc gggccttcaa
    12661 aaaggacctc cacgccctgg tggagaacat gaagaagctg gtccgcctgc agaccgagag
    12721 cgcggacttc aagggccgac tggaagccgc cacccgggag gtggagaccg aggaaacccg
    12781 caggctttcc gagttcgagg ccgagctggc cgcccagggg ttccggatcg cccaggccca
    12841 ggacgaggaa ggctccacca ccacggacat catccccctg cggaacgggg agccctcgag
    12901 cttcgacgcc ctccaggccg aagtggcctc gggcggcctg gcggaggagg aatggaaccg
    12961 gctccggggg ctctaccacg agtccatgga ccgcctgaag tccctgttcg ccgaattgcg
    13021 cagggggcgg gtggagctgg agcggcgcct ggagaacctg cgccacgagg ccctccggcc
    13081 cttcgtactg gcggagacgg agctcctgcg gaagcgctac ccggacccga ggatccacgc
    13141 ctgggtctcg gacctggaga gggacctcct ccagcatctc cggctcttcc tggccgaccc
    13201 ggagcccgag cctcctctcc ccgcacgacg ccccgtgccc cccctggccc ggtacgggct
    13261 caacatcctg gtggacaacg ggcaggccga gcggcccccg gtaatcctgg aaaaccaccc
    13321 ctccagcgtc aacctattcg gcttcgtgga gccccggccg gacgagcgag gggacacccg
    13381 ggccgcgtac ctcaagatac gccccggggc cttccaccgg gcggacgggg gctacctcat
    13441 cctccgcgcc gaggacctgg tccaggacga ggaagcctgg ctctggatga agcgggtcct
    13501 gcagaccggg gcggtggaga tccagccctc cgcggggccc ttcggatccc ccggtgcccc
    13561 caccaagccc caggccgcgg aggtggacgt caaggtcatc ctcctgggcg gggagatgac
    13621 ctacgacgcc ctctacgccg cggacccgga tttccagaaa ctcttcaagg tctacgccga
    13681 gttcgactcc accatgcccc ggaacgacga gaccatgcgg gaatacgtgg ccttcatccg
    13741 gaagatcgtc cgggaggagg ggctattgcc cgcggatccc gacgccatcg ccgcggtggt
    13801 ggagtacggg gtcaggctct cggagttccg gaaccgcctg tccacccggt tcagcctgat
    13861 cgcggacctc ctccgggaag cttcctggca cgccgggcgc gccgggcgga ccgccatgga
    13921 cgccgggagc gtgaccaccg cgatcagcgc ccgggcctac ctggccaacc tgccggagga
    13981 gaaggtggag gagatgatcg cctcggggca gatcatcctc caggtgtccg ggtcggccgt
    14041 cggccgggtg aacggactcg cggtccacga ccggggctac tacgccttcg gcctgcccgc
    14101 ggtgatctcc gcccaggtct ccccggggga gagcggggtc atcaacatcg agggagaatc
    14161 cggactttcc ggggagatct acgacaaggc cgtcctgatc gtcgagggct tcctccggag
    14221 ccgctacgcc cggggcttcc ccctcatggt caccgccagc atctgcttcg agcagtccta
    14281 caccgccatc gagggggatt ccgcgtcctc caccgcggtc tacgccctcc tgtccgccat
    14341 agccggggtt cccctccgtc aggacatagc cgtcaccggc tcggtcaacc agatgggcca
    14401 gatccagccc gtggggggga tcaccgagaa gatcgagggg ttctaccagg tctgccggag
    14461 ggcggggttc acgggtaccc agggggtgct gatcccccgg cagaacgccg tgaacctgac
    14521 cctgtcccgc ccggtccagg aggccctgcg ggagggccgc ttccacatct gggcggtctc
    14581 caccatcgac gagggcgtcg cggtccttac ggggatgagc gcgggcagcc cgaacgagaa
    14641 gggagagttc ccccccgaca ccttcaacgc ccgggtccgg aaggaactcc agcgcatggc
    14701 ccggaccatc aagtcctatc tggggtagaa gcatggttta ttatttgaaa attcagattc
    14761 gttccgttat cctaggaaac gataccatcc caacaagtgt gaaaggtctg gaccatggcc
    14821 gcaaatgaac tcgtagtcga cgtctccaag atcaagaagg cggtccacag cgccatcccc
    14881 ctcacgatca cgacctacac cctcccccac gagatcgaga tctacctgga ggaggtcctg
    14941 gacgtgttcc tgggcgaact ggggcagaaa aagctcaagg actacctggt ctactgcctc
    15001 cgggagctgg cggtcaacgc caagaaggcc aacaccaagc gggtctactt cgattcccgg
    15061 ggcctttcca tcaacgaccc ctcggactac gaggagggaa tgaagagttt caaggcggac
    15121 accctggaga acatcgcctg gtacctggcc aagcagaagg agaaagggta ctacatcaag
    15181 atcgtactcc aggccaaggg atccaccgtg gtcctggagg tccggaacaa cgtcgagatc
    15241 aaccgcaccg agtacgtccg catccacgac aagctggccc ggtcccgaaa gtacaccagc
    15301 ctggaggaag ccctccagca ggttctggac ccctcggaag gcgcgggcct ggggctcgtg
    15361 atcctcgtgc tcatgctcaa gaagatcggc ctggacgagg agtgcttcga catccgggcg
    15421 gagagcgggg agaccctggc gcggatcacc gtgcccgtgg aggccacccg gatcgagtcc
    15481 ctctccaaac tcacggacga gatcgtacgg cagatcacct ccctgcccca cttcccggac
    15541 aacatcgtgg cggtccagaa gctcctggcg gatcccaaga gcgagatggt ggacatcgcc
    15601 cgtcacatct ccacggatcc cgccatgacc gcggacctcc tcaaactggt caactccgcc
    15661 cagttcatgc tgcccaagcg ggtggacaac atcgtggacg ccgtgaaact cgtgggactc
    15721 aagggcatcc gtaaccagct gtactcctac ggggcccaaa agcttttagg ggacgacacg
    15781 gacaccaaga agaagctctg ggaccactcc taccgcaccg cctattacgc ctacaacctg
    15841 gtcaagaact tcaagcccgc caagcggcac atcctggacg acgcctacgt gggcgggatg
    15901 ctccacgaca tgggcaagat cgtcttctcc acggtccacc cggacctcct ggaccgcatc
    15961 cgcgccttct gcggggaaaa ggacatcccg gtccagacct tcgaggacat cgcggggggc
    16021 atgaaccacg cggagatcgg ggcgcggatc gcggaaaagt ggaacttccc cgaatccctg
    16081 gtggcctcca tccgcttcca ccacgagccg ggattgatga ccatggacta caaggacgtc
    16141 gtgttcgccg tgtacctggc caacgccttc tgcgagatcg agaacggggg catgacctgg
    16201 gaccagctgg aacccctggt catccaggac tacgggttcc agggggaggc ccagttccgc
    16261 aagatcgtgg aacagttcag cgagggattc aacaaggaga cccgcaggct ggccggggag
    16321 gccggcaaga aggcctagga gtttgtcggg ctcgcccacc tagatagtag aaaaatcgca
    16381 tttgtgcgat ttttctacct tgcaaactcc aaaggagccg tataatcggg atttttagca
    16441 cctatcggcg ctaaaaatcc acaagtccga caggctccta gccgcggacg tcttcggagg
    16501 aattcccgtg aacacccccc ccgatcccgc gggccccggg tccgacggcg acgcccgctc
    16561 cggcggcctt gccgaggcgg ccttccggct gaacgccctg ctggaagccg ccatgccggt
    16621 catcaccccc acgggggtag ttctcgggtt cctcttcccg gatttcttcg tccgcctgcg
    16681 gccctacatc ccctggctct tcgcgtgcat caccctggcc ggggccctga agctccgggc
    16741 ccgggacctg ggcgcggccg cggccagccc gatccccgtc ctggacttct tcgcggccgc
    16801 ccacgtcctc atgccggtgc tcgcctgggc ggtgtcccgg ctggtcctgc cgggggacac
    16861 ggaggccgcc gcgggcttcg tcctgctctt ttccgccccc accgccgtcg cggggttcat
    16921 ctgggtatcc atctaccgag gggacgaggc cctggccctg gccttgatac tcctggacac
    16981 cctgggcgcg cccatcgtca tgcccttcac cgtctccgcc ctcctgggca ccgggatccg
    17041 actggacggg actgccatgg cggcctccct ggccctcatg gtggtccttc ccacggtcct
    17101 gggggtggcc ctcaacgaag ccagccgggg ccggatcccc cgggccgtcc gcccctacct
    17161 gacgccgctt tccaagatcc tgctggccct ggtcatcgcg gccaactccg cggccgtcgc
    17221 gccctccgtg gacttctccg aggcccgggt ctgggctgtc ggggcggccg gtatcgggct
    17281 gaccaccgcc gcctttttcc tgggaaagac cctgggactc gtgcccggga tcggccccgc
    17341 ccgaaggagg ggcctcgtgt tctccgtggg tctgcggaac atcagcgccg tggccaccat
    17401 cgccatcacc ttcttccccg aggcagcggc cctgcccgcc gtactgggga tgttcttcca
    17461 acagaccctg gccgctgtct tcgggcggat cctgctgggc aaaccctcaa aatcccgaga
    17521 atagcctttc caggaagacc agcatccagt gctggaggcc gaagaactcc gcctccaggg
    17581 ccgcggcctc ctttccgacg taccggtagt gccagctttc ccagtcgtac ccggtcaggc
    17641 cctcggcccc ccgggggtac gacagggaaa aaccgaagcg gcccgcgttg gcggccatcc
    17701 agagccccgc cttggattcc gcgaaggtat tgtcgatgga tccgaaatcc accacggttc
    17761 ctagctgatg ctgggaccgg ccgggccggg cggaaatacg gtccgcggct gcctcgccca
    17821 gctcccggac gtgccgttcg tagacctcgg actggtacgc gtaggatcga tatgcggagg
    17881 agaagaccag ggtcaccccc gcagccttgg ccgcggcgtc cagctcccgg gcggcctccg
    17941 cggcgggctt ccgaaggcgg aggtcccggc gggccagggc gaaagggtat tccgcaaggg
    18001 aaaccaggtc cccgggctcg tagtcggcgg ggaggggctt ctccttgtcc acccgagcaa
    18061 ggtacagggg gtcctccacg gcccagatcc ggcggagaag cccggcaaac cgtaccggag
    18121 cggcttcggc ccgggccatg atctccgggg ggaggcccgc cagggcggcc cggtagggtg
    18181 ccagggggtc cgagggcggg gatttggggg ccggcggcat gcaggccgcg agcaggatgc
    18241 aggccgtggt cagcagggcg tatcgcatgg ctctgaggat acggcgaacc ggatcgcgga
    18301 ggcaaccggg gcgcgggacg tgaccgatcc gcctcgtgcg ggtatactcc ttgacatgcc
    18361 gtcctcgttc cgccctgccc cgcgccgcgt cctttccgcc gccctccgcc tcgcccttgc
    18421 cgcctccgcc ctggctctcg cggcgtcctg cgggtccgga ggttcggggc gttacaccgt
    18481 gcccgggctc gagggcgtcg gcccggaatc ccggttggtc cggcaagccc gggaggaata
    18541 tgtggacctt ccgggcgtac gggggccgga ggccgcctct ccgaagcccg gggaagtccc
    18601 ggcggactgg cggacctacg gggagggctc tccctcccgg ttggccgtcc tcctgacgga
    18661 tccccggtcc tcctggctgg gcctggtcca cggcttcaag tccctgggca tacccttccg
    18721 cgtcacggat tccctggact ccgccctgga tcacgacgtg gtcctggcct acccgggttt
    18781 caagcggggg agcctggacg acacggaggc gcggcggctg gaggatttcg tgcgcggggg
    18841 cgggaccctc ctggcggtcc agaccggccg gccggagccc cgggtcgagg ccctcttcgg
    18901 gttccgggga gccgtccgga accggcggag gtccgaactc cgctacgagg acagcccctt
    18961 cgccgccggc ctcgagtatc ccgaggagcg gaccacgcgt cttggcaatc ccgaccgcgc
    19021 ccggtacagg ctccgtacgg cggccttctc ggacccggag gaagtgctcg cccggtacga
    19081 ggacgggggg gcggcggtca tccggcgccg tgacggagcc gggaccgcga tggccttcgg
    19141 cttcgacctc ggccggtaca tccagggcgc ccaggggaac cgggacgacg aggccaaccg
    19201 cacctacgtg aactccttcg agccctccgt ggactccctg ctccggtttc tcggggaggt
    19261 gtaccgcgcc cgggagccgg acgcggtctt cctccacccc gccccggacg gcatgcccct
    19321 ctcggtcgtc gtctcccacg acgtggacta cgcggggtcc ctggccaaca gcgtggagta
    19381 cgcggagtac gagcggagca tgggatacgg cgcgacctat tttctgcaga ccaagtactt
    19441 ccgggatttc ttcgacgagg tcttcttcga cgagcggacc ccggagctcc tggcacggct
    19501 ggaggagctg gggatggagc tggccagcca ctcggtctgc cattcggaca tgttcgcccg
    19561 ggtgccgcag ggaaccttcc gggagtccta ccccgactac cagccccgga tcatcgagtt
    19621 ctactacacg aggaacgcga cgatcatggg ggagctgcgg gtcagcaaat tcctgatcga
    19681 gcgcctgtcc cggtcccggg tcgattcgtt ccggcccggg ttcctggcca accccttttc
    19741 cctgccccag tccctggaat ccgcggggta ccggtattcc tccgcggtgg cggccaacga
    19801 cgtgatgacc cacctgccct tccgcctgaa ccacgaccgc ctgtaccggt cggagaccgc
    19861 ggtctacgag tttcccatca ccgtggagga cgagaaggat ccgcccatgg acggccgggt
    19921 cgaggacgcg gtggagctgg cgggaaagat ctcccgctac ggggggctct tcgtcatcct
    19981 catccacccc aacgtaaccg cggagaagct ccggttcctc cgggccttct cggagaggat
    20041 gcgcgaaaag gcctggtggg gaaccctgcg cgagttcggg gactggtgga cggcccggga
    20101 cggggtcgag tccgacgtgg tcccttccgg agaccgccgg gtactccggc tctcctcgcc
    20161 ccttcccgtc cggggactcg gcatacggct tcccgaaggt ttccgcatcg ccgcgccccg
    20221 cggggcgaac ggcaccttcg tactcgacct gcccgcgggg actacggagc tggtcctgga
    20281 accctagacg gcaggaatcc cttccgctcc agcttctgcc aacccttgtt ccgggtcagg
    20341 agctggagga cggaaaacac ccggacccag cccatgagct ggcggaaccc gaagttctcc
    20401 aggatggagt accccaggag ccgggccagc tccacgggtc ggaagtacac gatatcccgt
    20461 tcggacaggg ccagggagac cagggagatg agcagtccga acaggaggac cgcgctgaac
    20521 atgaccagga agacgggaag gctcagggcc ccgaccgcga agccgaggat catgagggga
    20581 tacccggcga actcgtagaa ggggccgatc acctcgaacg cgaggaaata gggaaaggcc
    20641 agcagccccg ccgatccgta cttcggattg aacaacatct tccggtgcca ggtcatgatc
    20701 tccaccaggc cccgatgcca gcggtccctc tgcttgtaca gccccccca
//
LOCUS       DAOTEI010000045        20390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1736_length_20390_cov_4.754119, whole genome shotgun sequence.
ACCESSION   DAOTEI010000045 DAOTEI010000000
VERSION     DAOTEI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 20390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20390
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1736_length_20390_cov_4.754119"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="P5313_06310"
     CDS             <1..863
                     /locus_tag="P5313_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80008.1"
                     /translation="RSGGGILFRAEADSPGPALELFLTEGRPSVILRSGDRAGEVRSA
                     ALLVPSRPVQLGVTLRPEGPALRISFLVDGTPAGEGSLPFGASGWAGDGTTTVAGPDG
                     YPGLYDEVGVWAFDDLGDPSVYPAFLYASRRSLGPALVWAEGFEGTYGPPSVLSGGAS
                     VEAGAGLALPAGSGARFGQLPEGTLEVEAALSAGPAPALVIHGSEGSVVLPWKPGVLD
                     MDPGTGLPLLRARIRPAEEGGGLTVSSGEETVRVSGAGPWSLGLAGPGAGISRVRSIR
                     VYRPARAPGE"
     gene            1016..2092
                     /locus_tag="P5313_06315"
     CDS             1016..2092
                     /locus_tag="P5313_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80009.1"
                     /translation="MKQRAILAAILAAFFLLSGCVSAPPPAPAAPADSGSSAPASSAD
                     KDRAKALVQGGGDAGSQVPPVGAPVGAPASSPATPAAAPAATADVPPPPPGKLTPEEE
                     VFLQNYLARLNYMVYYDEDAGIEKIYAKTAVNQANRYLIERMGLSVLDFDQVEKNKKD
                     QQAAYQSETGGAIDLIQYLAQKYNADAYVELSFTATPSASGGRFYATAQGSMKIFDTS
                     TGTLLGSVAFQSPQVMNPVSQESAISNALAASVWNAMPKMTEQAKALIQGSLARGIRY
                     ELTVQNTPDSKAMSDFRRALARQVREVEQVSYSAAETKLFVYTFQSRDKVEDAVYAAA
                     ERAGMRDLYLVFNRGKAFTFNTGR"
     gene            2233..2895
                     /locus_tag="P5313_06320"
     CDS             2233..2895
                     /locus_tag="P5313_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80010.1"
                     /translation="MKKTLYVLAILLMAFAAMGCASKPSAPPPIATNTPPYDILQHKG
                     TTLGITTLPGWIEASLQGPKAVERLADYKDKFVVVVDVTGRDLEGTSLAAQRLNADPE
                     ISRFLSIRVKDTFAGAQVGDKDKIETYMERVVKSVSEARFSGFLKAADWWVQIRWYKP
                     DGKKTFDRDEFRVLQLYTVDKAVLEQQLQKILSGEAAAEPKTPEKERAMQAVQQAFYE
                     GF"
     gene            2975..4450
                     /locus_tag="P5313_06325"
     CDS             2975..4450
                     /locus_tag="P5313_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80011.1"
                     /translation="MIKRFAALSFAVLLLASCVSTPAQTKAPAWTLTTPSPDGTYTYF
                     VGYSADPTGDAAKATEGATANLLSSIMNYIGVKITVDSSATARASYDSYQADIVQTVR
                     TQSTNRLAGFMVKDKHQIKEKGGRVTVYILASYITKDLEAEKTRIQRLFQEQIDAVAV
                     PERKGQSLESEGRYFEAVQSYIEAAVAASGADIDNADIKLERNVNNARRVLQRLRFVA
                     AGPAPKAGLGQPFSGPFAARLVYGENDSGPGIPGAEAYVTYQVRQASGRLTGKTERAV
                     SDSRGLFSFTPPPPNFVGGSKLIMRLNLDSTLDLLDKFPAKFESFRSSLETELRGRVI
                     EFEYTVVSIAKDVATGIALVDLDEKGAAVGGNQAQAGLIEAMIRERFKVQAAPLDAAL
                     LSGGDDAAILAAAQRLYRARFGRIVAGTARIESVRKDGSNFMVSVRASVKCWDLATGD
                     ILYSADKAYTGVGPDEAAARRASLQQIGRDTLGRDLMANLP"
     gene            complement(4608..5363)
                     /locus_tag="P5313_06330"
     CDS             complement(4608..5363)
                     /locus_tag="P5313_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRY80012.1"
                     /translation="MSRLPLLPDFPRPLLFAHRGVSSVAPENTRAAFALARQLGVPGV
                     ELDVHRCASGELVVTHDYSTKRVTGTELEIESTPWERLRDLDAGSWKGPEYKGERLPL
                     LADLLEEFGADLYWDIEIKSRVHADAELEEALARLLDSPRLAGRLAVSSFNPFVLGRF
                     KALSPKIPTAIIWCRSKEQPWYLRHGEGRWIAGTDFLKPDKALVKTLPLPGGRERAAW
                     TVDEPAEARRLLSLGCSGIISNRPHELGIPLGR"
     gene            complement(5367..5834)
                     /locus_tag="P5313_06335"
     CDS             complement(5367..5834)
                     /locus_tag="P5313_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein"
                     /protein_id="HRY80013.1"
                     /translation="MTDDTDLSGLDYPSARAYALEFMTALKTAEKALEAARQEVRLWT
                     DRAALAASKGMADLETAARAKAGEAEAKAASLEAEKADLARKITRIREKLPVLKASER
                     SVDADLLLAELQMATGEALNPESAALAELDKTLQAGAADAALEELKRKLKGES"
     gene            complement(5831..7192)
                     /locus_tag="P5313_06340"
     CDS             complement(5831..7192)
                     /locus_tag="P5313_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80014.1"
                     /translation="MRKVIAALLLTALALAPLAAQPKVSEKQEVAIFAMGYYGWNIPR
                     EALGSIDLEIQKVFLDLGRFTVMGYTQRFTSTDVQSFVDILKKAKETNFVLPEKYQFG
                     EAFLTAAEFEKLLGAFQVAIPVVVSFDSQYDSKDNRWETNIKTSISFVDAGTGTLTGI
                     ADVQTSGTDKSSQLASIQSAIGSIPMELQYKIRSIPAFTLNTRVLAASGKEIKLQLGT
                     NMGIKKGDEYSIIVASQVAGFQDEREQGLVLIKDVGPEVSTGLVLYSSAKPGPDTQLR
                     EIPRRGVDAAPYIHFAQGSLLRVIGDPVQPESEAEGSNILLGLRMPLSRGFFDVRPYA
                     AIQVPVNGIRSILSFVFVPVNVLLGAEYNLYLGRLAVTPYAGIGTSFVYITEAFSGST
                     YDTSDMLFPHFGAQAYLNVSYLFNRNTKLFVEAGFEYWLALTSLYSNYGGVSFGGGVS
                     WKL"
     gene            7448..8905
                     /gene="mnmE"
                     /locus_tag="P5313_06345"
     CDS             7448..8905
                     /gene="mnmE"
                     /locus_tag="P5313_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRY80015.1"
                     /translation="MPSGYLRLDVPIAALATAPVPAALAVVRTSGPGAPELAAKAFSR
                     PEALSRAETHRAVHGFVRDPETGRNADEVVALVFRAPSGYTGQDAVDFVCHGGPAAWE
                     GVLTALEKAGFERALPGEFTFRAFAAGKMDLAAAEAVDELVRARTGEARQEALERLSG
                     GLGREVSDLRDAVLAALTEAEARLDYGEDELADGPDDLLAAVRAARSRAEALAASHAV
                     GRLLAEGARVALAGPTNAGKSRLFNLFLKEERSIVSESHGTTRDYIEADLDLDGIPVA
                     LFDTAGLREAPDPVEAEGVRRSRLLAEGADLVVYLVDGTRGAGPGDLDTLGSLPGAVR
                     VWNKVDLPEALPAPEGWIPLSAATGEGFPALAAELGARLRGGALSGNARGTELDRGAS
                     GDLDEAQRASSPPISGRPLPHTAPRLASRRQADCARRAARALAAAGAALAAGAHLDAA
                     AVDLREAADALAELTGESVPESVLEMIFSRFCVGK"
     assembly_gap    9217..9316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9405..11318
                     /gene="mnmG"
                     /locus_tag="P5313_06350"
     CDS             9405..11318
                     /gene="mnmG"
                     /locus_tag="P5313_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRY80016.1"
                     /translation="MDYEAIVIGGGHAGIEAASALARLGHRTLLVTQNPDAIGRLSCN
                     PAVGGLSKGNLAREVDALGGEMGLLTDASMIQYRVLNRSRGAAVQAPRAQVDKTLYSA
                     RARRRLESLSGLDIRMDTVTNILTDRSGTRVEGVVTERGERIGAAVVVLTTGTFMEGR
                     LFIGEWEASGGRLGEGAALGLGTELRAKGFTVGRMKTGTPARVRGRSLDYGLMERQEG
                     EPPRPFSFRNDGVVRPSVPCWITYTNEASHQAIRAGLDRSPLYGGKITGRGPRYCPSI
                     EDKVVRFPDRTRHQVFVEPEGLDTDEMYLNGLSSSLPEDVQEAFLRTIPGLERVEIVR
                     PAYAVEYDYLDPLQLRPSLETKRIRGLFTAGQTNGTSGYEEASAQGVLAGINASRRLR
                     GEEPLVLPRSESYIGVLADDLTTLGTQEPYRMFTSRAERRLSLRCDTADRRLTPLGRR
                     EGLVDDAAWERFEARRAGIEEAAELLRTRKVTAAHAARVEALSPEQATERVLSPPPPG
                     VPRAPSPLAPHVGESWDRAVRDPRAFGLDLVDFLPELSALSPDQVETAVLDARYEGYA
                     AREARQAARLGRSEDQRIPEGFDYRTVEGLSSESREKLEAVRPLTLGQAARIPGIRPA
                     DAALLMVKLARGE"
     gene            complement(11335..11676)
                     /locus_tag="P5313_06355"
     CDS             complement(11335..11676)
                     /locus_tag="P5313_06355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRY80017.1"
                     /translation="MRRGEIRWADLGVPSGSAPGFRRPVLLLQSDSFNDSRISTVICA
                     VLTSNLRLAEAPGNVLLESEESGLDRDSVVNISQVVTLDKSCLDSRVGLVRPSTLRRV
                     EEGLRLVLDLE"
     gene            complement(11741..11905)
                     /locus_tag="P5313_06360"
                     /pseudo
     CDS             complement(11741..11905)
                     /locus_tag="P5313_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
     gene            complement(11989..12276)
                     /locus_tag="P5313_06365"
     CDS             complement(11989..12276)
                     /locus_tag="P5313_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRY80018.1"
                     /translation="MSGSTFPSPGEILRKRFMVPREISIYRLAKDSGIPATRVSGILR
                     GRRKINAESALRLARYFGNDPEYWLDIQNRYDLAEERRRLTDALYKIRSVS"
     gene            12615..12857
                     /locus_tag="P5313_06370"
     CDS             12615..12857
                     /locus_tag="P5313_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HRY80019.1"
                     /translation="MKTLPVGELKTQFSEVLERVRQGESFGILYGKRKKPIALIIPYT
                     EKTSQEERALGFLEGKVTVKFAADFGITEEELLNPA"
     gene            12854..13252
                     /locus_tag="P5313_06375"
     CDS             12854..13252
                     /locus_tag="P5313_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY80020.1"
                     /translation="MKYLLDTHSFLWSIADSGKLTKKSIRILKNPGNEVFVSSVTLWE
                     IAIKTRLGKLEIEGFSLDQLPGIIEHLRFGRIDCTMEDAIGYAGLKEPTHKDPFDRML
                     ILQCIRRGLTMISADAEFPKFVPYGLKLIW"
     gene            13670..14629
                     /locus_tag="P5313_06380"
     CDS             13670..14629
                     /locus_tag="P5313_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80021.1"
                     /translation="MKKLFVLLLALAVVTGAFAQVTTSISLQGQVKVLNEDSQGQFVP
                     KGDTYDTFTFKASTDTYGFSMTDTNWLTGLTAPDGFQGFGDIRDWNVWYKLADGKVKV
                     TFGNLRDATFRTTLTGWLADFGAMDRIAGYGLFTTWMPMDGLTVGLNLPFGLTAEDTA
                     DVLQKADIGLQYKIADVGTLNVLVDLNMVGDDNVFELGFAYTGVENLTATAIYKGELD
                     AEIHSFVVEGDYVMDALEAGASFMGIADASTDFAWEVNAYGYYDITEKIYSGLEIYYW
                     SDETYDFWGNLGYNFGNGLKADLSLGYNADFYYAVALKYAVTF"
     gene            14700..15287
                     /locus_tag="P5313_06385"
     CDS             14700..15287
                     /locus_tag="P5313_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80022.1"
                     /translation="MVQIKLKPGFVVLALCLVGIVVSCAGSPEAAEGDVIQVSLLTDR
                     QIRNIYGTELQENPFLPPAGLFTRSKMQFFVLRFEGRSEYINIENVKVRYGDFFAKSK
                     IFGPEDLKAFWEARDTDTDDIKSQALLAKKYRTISTFVIDPYKPFRIKTNLSKVMVIV
                     SEIPSESEAVPILELAFNNGQESFSNEYTGVELTM"
     gene            15377..15982
                     /locus_tag="P5313_06390"
     CDS             15377..15982
                     /locus_tag="P5313_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY80023.1"
                     /translation="MGQCSRRYSPGDRAYLLFVASKVSLSLRIPGMRKPRELHDGGLY
                     HVTARANRKEMILDNRGMKDLFLQVVARARKRYDFRIENFALMGNHFHLMIRPGRRES
                     LSRIMQWILSVFAMAYNRIHGYVGHVWGERFFSRIIRTFAQFLMVFEYIDRNPVEAKQ
                     VDQVWEWEYGGLWHRRTGRRDPPGTELSLVKARLDSPRPVP"
     gene            16004..16402
                     /locus_tag="P5313_06395"
     CDS             16004..16402
                     /locus_tag="P5313_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRY80024.1"
                     /translation="MANRANDWLRQAEEELLWAKDALVSGRWALVCFTSQQVAEKSLK
                     GMAIRRGAAQIKSHSVKDIAAALEIDGELDEMGRVLDQYYITTRYPDAFYSGSPFEYF
                     TKGQAAEALRYAQAFLDRARAEIRGPDAAT"
     gene            16399..16755
                     /locus_tag="P5313_06400"
     CDS             16399..16755
                     /locus_tag="P5313_06400"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY80025.1"
                     /translation="MNPDEPVIVWNTRDPLGGLTPAQFEAELRNRLAGRVEAAYLFGS
                     YGTPEFDRDSDVDLFLVVRTAKPFLDRFLDFTDIQDLVPAMDLLVYTPDEFESLTSDP
                     SPGFWTSAVASLRRVL"
     gene            complement(16762..17847)
                     /locus_tag="P5313_06405"
     CDS             complement(16762..17847)
                     /locus_tag="P5313_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80026.1"
                     /translation="MRSFDRVPVRFLAFFSALSLLLIAAALPAAAASPPLVFTKYLDK
                     TEGAFFVLVPKGWITQGGMVRVNPLAAQGGVGNATEAKIDFAVAKDATGKVQIRWIPK
                     INYAQPSPSNAMLGGNWNGMPVVAYPRAADFLGRMLFPALHPQARNVKVVETAQRPDI
                     VAAVYQMPAAQAIRSQGGAFHADAATLTVTYEEGGVRFKEILFAAVEGYAMMGAGLWD
                     NPFTIVARAPEAEFEVWGPVARTVVNSFALNPLWLQGEMAGQARRADIAAATLRDISR
                     VDAEIAKSRSQTMAAIQQDQYLTLTSQELYVNPRTKEVELGSNEWKHRWVGPGGEVIY
                     TDDGSWNPNLDPSLKLSGFERSPVKKR"
     gene            complement(17885..19855)
                     /gene="tkt"
                     /locus_tag="P5313_06410"
     CDS             complement(17885..19855)
                     /gene="tkt"
                     /locus_tag="P5313_06410"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY80027.1"
                     /translation="MDTAALSKVALTVRTLSMDAIQKANSGHPGLPLGCAELGALLYG
                     EILKHDPSDPAWLDRDRFVLSAGHGSMFLYSLLHLAGYKVSLDDIRAFRQIGSPCAGH
                     PEYGHCPGVETTTGPLGQGIAVAVGMAMAERMMAARFNTPAHPVIDHWTYALAGDGCL
                     QEGVSAEASSLAGHLGLGKLVVFYDSNKITIDGGTELSFTEDVARRYQAYGWKVLSGD
                     MYDFAGVSRLVAEAKTDLERPKLIILKSVIGKGAPTMQGSHKVHGAPLGAEEIKRARE
                     AMGVPADADFYVAPEAVSYFEGRRTAFRKTREAWNALFAAWKAANPGKAKELDAFLAG
                     KASAPFAAPAFAAGESLATRAASGKALAAAAAAWPNLVGGSADLTGPNSSQLPEGGVF
                     SRANPAGRMIHYGVREHAMAAVSNGLALHGGLRPFCATFLVFADYLRPSLRLAALMKL
                     PVIYILTHDSIFVGEDGPTHQPIEHLASLRCIPNVRVLRPADPEETAEAWRLALERTD
                     GPTVLALTRQNLPVLAKADPDWRETMKLGAYVVRNSKAEPDVVVLASGSEVPLALAAA
                     DKAVGRDVRVLSVPSLELLRAADPAVLRVLMPTGARVVAVEAGTAWGWDALTTTPKDV
                     LSIDRFGASGPGDKVAAHLGLTVEKLAGLIAG"
     gene            complement(20006..>20390)
                     /locus_tag="P5313_06415"
     CDS             complement(20006..>20390)
                     /locus_tag="P5313_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY80028.1"
                     /translation="TVTVMNEIRDMGVRFAVDDFGIGFSSLRYLDQLPVDVLKIDKAF
                     IDPITDRYEGAQIATAVIALARSFEMISVAEGVETAEQLEALRMRGCDEIQGYLVSKP
                     LDARAFRAFAFPAGGAGRPDPDPSA"
BASE COUNT         3455 a         6520 c         6789 g         3526 t
ORIGIN      
        1 cccggtccgg agggggcatc ctgttccggg cggaggcgga ttccccgggc ccggccctgg
       61 agcttttcct gaccgagggc cgtccgtccg tgatcctgag gtccggagac cgagccgggg
      121 aggttcggtc ggcggccctc ctggttccat cccggcccgt ccagttgggc gtgacccttc
      181 ggcccgaggg cccggccctt agaatctcgt tcctcgtgga cgggacgccc gccggggagg
      241 ggagcctccc cttcggcgcg tcggggtggg cgggcgacgg gaccacgacg gtcgcggggc
      301 cggacggata cccgggcctc tacgatgagg tgggcgtctg ggccttcgac gacctcggcg
      361 acccctccgt ctatcccgcc ttcctctacg cctcccgcag gtccctgggt ccggccctgg
      421 tctgggcgga gggtttcgag gggacctacg gtcctccctc cgtcctttcg ggcggggcct
      481 cggtcgaggc gggcgcgggc ctcgcgcttc ctgccggatc gggcgcccgg ttcgggcagc
      541 ttccggaagg taccctggag gtcgaggccg ctctttccgc gggcccggct cccgctctgg
      601 tgatccacgg ctccgaggga tccgtcgtct tgccgtggaa acccggagtt ttggacatgg
      661 atcccgggac cgggcttccc ctcctgcggg cgcggatccg ccctgccgag gaaggcggag
      721 gccttacggt gagctcgggt gaggagacgg tccgcgtttc cggggccgga ccctggagcc
      781 tgggcctggc gggccccggg gccggtatct cccgggtccg gtcgatccgg gtgtaccggc
      841 cggcacgcgc cccgggggaa tgatccgccg gccttgattt tccggagcga ttcactagat
      901 tgtaaacgga cgccttcacg aagtcggaag gatccgggat gccgggcagt gtacgcatgg
      961 ttttccggac cgtcggcgtg ctatattcct agagcgtacc ggcaaggagt cttgaatgaa
     1021 acaacgtgcc atcctggccg cgatcctggc ggcgttcttc cttttgtcag gctgcgtaag
     1081 cgctcctccc ccggctcccg cggcgccggc cgactccgga agctccgccc cggcgtccag
     1141 cgcggacaag gaccgggcca aggccctggt gcagggcggc ggggacgcgg gttcccaggt
     1201 tccgccggtc ggcgcgcctg tcggcgctcc cgcatcctcg ccggcgaccc ccgcggccgc
     1261 cccggccgcg accgcggacg tgcctccccc tcccccgggc aagcttaccc ccgaggagga
     1321 ggtcttcctc cagaactacc tggcccgtct caactacatg gtctactacg acgaggacgc
     1381 gggcatcgag aagatctacg ccaagaccgc cgtcaaccag gccaaccgct acctcatcga
     1441 gcggatgggg ctgtccgtcc tggacttcga ccaggtggag aagaacaaga aggaccagca
     1501 ggccgcgtac cagtccgaga ccggaggggc catcgatctg atccagtacc tggcccagaa
     1561 gtacaacgcc gacgcctacg tggagctttc cttcaccgcg accccctcgg cctcgggggg
     1621 acgtttctac gccacagccc aaggctccat gaagatcttc gacacctcca cgggcaccct
     1681 tttgggctcc gtcgccttcc agagccccca ggtgatgaac ccggtctccc aggagtccgc
     1741 catctcgaac gccctggcgg cttccgtatg gaacgccatg cccaagatga ccgagcaggc
     1801 caaggccctg atccagggct ccctggcccg ggggatccgg tacgagctca cggtgcagaa
     1861 cactccggac tccaaggcca tgagcgattt ccgccgggcc ctggcccggc aggtccggga
     1921 ggtggaacag gtgtcctatt ccgccgcgga gaccaagctc ttcgtctaca ccttccagag
     1981 ccgggacaag gtggaggacg cggtctatgc agccgcggag cgggcgggca tgcgggacct
     2041 gtacctggtg ttcaaccggg gcaaggcctt caccttcaac accggacggt gatccgcggc
     2101 ggtcggttcg attcgaaaga ttccgggatc gggacgggga ttcccaaatt ccgggatgga
     2161 atagcggaaa gccggatttt tccgggagtc cgcgatagaa agtatcgtga cacgagtttt
     2221 taggaggtta tcgtgaagaa gacgctgtat gtcctggcta tcctcttgat ggcgttcgcg
     2281 gccatgggtt gcgccagcaa gccctccgcc ccgcccccga tcgcgaccaa tactccgccc
     2341 tacgacatcc tgcagcacaa gggtaccacc ctgggcatca ccacccttcc ggggtggatc
     2401 gaggcctccc tccagggtcc caaggccgtc gaacggctcg cggactacaa ggacaagttc
     2461 gtcgtggtcg tggacgtcac cggccgtgac ctcgagggca cgtccctggc ggcccagcgg
     2521 ctgaacgcag accccgagat ctctcgtttc ctcagcatcc gcgtgaagga caccttcgcg
     2581 ggcgcccagg tcggggacaa ggacaagatc gagacctaca tggagcgggt cgtgaagagc
     2641 gtctccgagg cccggttctc cggcttcctc aaggccgcgg actggtgggt ccagatccgc
     2701 tggtacaagc ccgacgggaa gaagaccttc gaccgggacg aattccgggt cctccagctc
     2761 tacaccgtgg acaaggcggt tttggagcag cagcttcaga agatcctctc cggagaggcg
     2821 gctgcggagc ccaagactcc cgagaaggag cgagccatgc aggcggtcca gcaggccttc
     2881 tacgaaggct tctgaaccgg cccttccatg agcgctcggg gcatctccgg tccgccgggg
     2941 gtgccccttg ccgtacccgg ataaggagat cgacatgatc aagcgcttcg ccgccctgtc
     3001 tttcgcggtc ctgctcctgg cctcctgcgt ctccaccccg gcgcagacca aggcccccgc
     3061 ctggacactc acgacgccgt ccccggacgg gacctatacc tacttcgtgg ggtactccgc
     3121 ggatccgacg ggggacgcgg ccaaggcgac ggagggggcg accgccaacc tcctttcctc
     3181 gatcatgaac tacatcgggg tcaagatcac cgtcgattcc tccgccaccg cccgggcctc
     3241 ctacgactcc taccaggcgg acatcgtcca gacggtccgg acccagtcca cgaaccgcct
     3301 ggcgggtttc atggtcaagg acaagcacca gatcaaggag aagggcggcc gggtgacggt
     3361 gtacatcctg gcctcctaca tcaccaagga cctggaggcc gagaagacgc ggatccagag
     3421 gctcttccag gagcagatcg acgccgtggc cgtgccggag cggaagggac agagcctgga
     3481 gtccgaaggc cggtacttcg aagccgtgca gagctacatc gaggccgccg tggccgcctc
     3541 cggggcggac atcgacaacg cggacatcaa gctggagcgc aacgtcaaca acgcccgccg
     3601 ggtcctccag cgcctgcgct tcgtggccgc cggacccgcc cccaaggcgg ggctcggaca
     3661 gcccttctcc ggccccttcg cggcccgtct ggtctacggc gagaacgact ccggcccggg
     3721 catccccggc gcggaagcct acgtcaccta ccaggtccgc caagcctccg gccgcctgac
     3781 cggcaagacc gagcgcgcgg tttccgactc ccggggcctc ttctccttca cgcccccgcc
     3841 ccccaacttc gtgggcggtt ccaagctgat catgcgcctg aacctggact ccactctgga
     3901 cctcctggac aagttccccg ccaagttcga gtccttccgg tcctccctgg agaccgaact
     3961 ccggggccgg gtcatcgaat tcgagtacac cgtggtatcc atcgccaagg acgtggccac
     4021 gggaatagcc ctggtggatc tggacgagaa aggcgcggcc gtcggcggga accaggccca
     4081 ggcgggcctt atcgaggcca tgatccggga gaggttcaag gtccaggccg cgcccctgga
     4141 cgcggccctc ctttcgggcg gggacgacgc ggccatcctg gccgccgccc agcggctcta
     4201 ccgggcccgg ttcggccgca tcgtcgccgg tacggcccga atcgagtccg tccgcaagga
     4261 cgggtccaac ttcatggtct ccgtgcgggc ttccgtgaaa tgctgggacc tggccaccgg
     4321 ggacatcctg tactccgcgg acaaggccta caccggggtc ggccccgacg aggcggccgc
     4381 ccgtcgggcc tccctccagc agatcggccg ggacaccttg ggccgggacc tgatggcgaa
     4441 cctgccatga cagccctttc cggaatcacc acggagggca cggagagcac ggagaagagc
     4501 tgatacagta caagacaagg tagggatgta gaacggatgg acaggattcg atcctgtcca
     4561 tccgtttttt tatcttgttc ggccagtccg tcgattcgtc cgacggctca gcggcccagc
     4621 gggattccca gctcgtgcgg ccggttcgag atgatccccg aacagcccag ggacaatagg
     4681 cgccgggcct cggcgggctc gtccacggtc caggccgccc gttcccggcc ccccggcagg
     4741 ggaagggttt tgaccagggc cttgtcgggt ttcaggaagt ccgtgcccgc gatccaccgg
     4801 ccctcgccgt gccgcaggta ccagggctgc tccttggacc ggcaccagat gatggcggtg
     4861 gggatcttcg gggacagtgc cttgaatcgc ccgaggacga aggggttgaa cgaggagacg
     4921 gccaggcggc cggccaggcg cggtgagtcc aggaggcggg ccagggcctc ctccagttcc
     4981 gcatccgcgt ggacccggct cttgatctcg atgtcccagt agaggtccgc cccgaactcc
     5041 tccaggaggt ccgcaaggag gggaagcctc tcgcccttgt actcggggcc cttccaggat
     5101 cccgcgtcca ggtcgcggag gcgctcccag ggggtcgact cgatctcgag ctctgtccct
     5161 gtcacccgct tcgtcgagta gtcgtgggtc accacgagct cgccggaggc gcagcgatgc
     5221 acgtccagtt caaccccggg caccccgagc tgccgggcca gggcgaaggc cgcccgggtg
     5281 ttctcggggg ccacggagga gacgccccgg tgcgcgaaca gaaggggccg ggggaagtcc
     5341 gggaggaggg ggagtcggct catgatctag gattcgcctt tcaacttccg tttgagttct
     5401 tccagggccg cgtccgccgc cccggcctgc agggtcttgt cgagctccgc gagggccgcg
     5461 gattcggggt tcagggcctc ccccgtggcc atctggagtt cggccaggag gaggtccgcg
     5521 tccacggacc gctccgaggc cttgaggacc gggagcttct cccggatccg ggtgatcttc
     5581 cgggccaggt ccgccttctc ggcctccagg gaggcggcct tggcttcggc ctccccggcc
     5641 ttggcccggg ccgccgtctc caggtccgcc atacccttgg aggcggctag ggcggcccgg
     5701 tcggtccaga gccggacctc ctgccgggcc gcctccaggg ccttctccgc ggtcttgagg
     5761 gccgtcatga actccagggc ataggcccgg gcggacgggt agtccagccc ggacaggtcc
     5821 gtgtcgtccg tcacagcttc cagctcactc cgccgccgaa ggagacgccg ccgtagttgg
     5881 aatacaggct cgtcagggcc agccagtact cgaagcccgc ctcgacgaag agtttggtgt
     5941 tgcggttgaa gaggtaggac acgttcaggt atgcctgggc gccgaagtgc gggaagagca
     6001 tgtcgctggt gtcgtaggtg gatcccgaga aggcttcggt gatgtagacg aagctggtgc
     6061 cgatccccgc gtagggggtg acggccaggc ggcccaggta gaggttgtac tccgcgccca
     6121 gtaggacgtt caccggcacg aagacgaagc tcaagatgct ccggattccg ttgaccggga
     6181 cttggatggc cgcgtagggc cggacgtcga agaagccccg ggacaggggc atgcgcaggc
     6241 cgagcaggat gttgctgccc tccgcctcgg attcgggctg tacggggtcc ccgatgaccc
     6301 ggaggaggga gccctgggcg aagtggatgt acggcgccgc gtccacgccc cgcctcggaa
     6361 tttccctgag ctgggtgtcc gggccgggct tggcggagga gtagaggacc aggcccgtgg
     6421 agacctcggg tcccacgtcc ttgatcagga ccagaccctg ctcgcgctcg tcctggaagc
     6481 cggcgacctg ggaggcgacg atgatggagt actcgtcccc cttcttgatg cccatgttgg
     6541 tgcccagctg gagcttgatc tccttgcccg aggcggccag gacccgggtg ttcagggtga
     6601 aggccgggat ggagcggatc ttgtactgga gctccatggg gatggacccg atggcggact
     6661 ggatggaggc cagctggctg gacttgtccg tgcccgaggt ctggacgtcc gcgatgccgg
     6721 tgagggtgcc cgtgcccgcg tccacgaagg agatggaggt cttgatgttg gtctcccagc
     6781 ggttgtcctt ggagtcgtac tggctgtcga aggagacgac cacggggatg gccacctgga
     6841 aggcgcccag gagcttctcg aactccgcgg cggtcaggaa ggcttccccg aactggtact
     6901 tctcgggcag gacgaagttc gtctccttgg ccttcttcag gatgtccacg aaggactgca
     6961 cgtccgtgga ggtgaagcgc tgggtgtagc ccatgaccgt gaagcgcccg aggtccagga
     7021 agaccttctg gatctccagg tcgatggagc ccagggcctc ccgggggatg ttccagccgt
     7081 agtatcccat ggcgaagatc gccacttcct gcttctcgct gaccttgggc tgggcggcca
     7141 agggggccag agccagggcc gtcaacaaga gcgccgcgat gacctttctc ataccgtctc
     7201 ctttatgtgt ccttgccgtt cgggggcgcc cgggcgagcc gagcctcgga gcgtgccagg
     7261 tgcaaatata ctgcggaatc gagcctgaaa accgagcttg aatgtcgaac tccgtccgcg
     7321 cgagtcgctc cgcgactcgc ggcgacgaat ccctgcggtt tccgtcgagg tctccgccaa
     7381 ggatatacaa agggtggaag atttgccggg ccttgtcaag gcgcgccggg gacggctatc
     7441 gtaccccatg ccctccggat acctccgcct cgacgtcccc atagccgccc tggccacggc
     7501 ccccgtcccc gccgccctgg ctgtggtgcg cacctcgggt cccggggccc cggagctcgc
     7561 ggccaaggcc ttctcccggc ccgaagcgct ttcccgggcg gaaacccatc gggccgtgca
     7621 cggtttcgtg cgggatcccg agacgggcag gaacgcggac gaggtcgtcg ccctggtctt
     7681 ccgggctccc tcgggataca cgggacagga cgcggtggac ttcgtctgcc acggcggacc
     7741 cgcggcctgg gagggggtcc tgaccgccct ggagaaggcc gggttcgagc gggccctgcc
     7801 gggggagttc accttccggg ccttcgccgc cgggaagatg gacctggccg ccgcggaggc
     7861 cgtggacgag ctcgtccggg cccggaccgg ggaagcccgg caggaagccc tggaacgcct
     7921 ctccggcggc ctggggcggg aggtttcgga tcttcgggac gcggtcctcg cggccttgac
     7981 ggaagccgag gcgcgcctgg actacgggga ggacgagctg gcggacggcc cggatgacct
     8041 cctcgcggcg gtccgggccg cacgatcccg ggcggaggcc ctggccgcga gccatgcggt
     8101 gggccgcctc ctggccgagg gcgcccgggt ggccctggcg ggccccacga acgccggaaa
     8161 gtcccggctc ttcaacctgt ttttaaagga agaacgttcc atcgtctccg agtcccacgg
     8221 taccacccgg gactacatcg aggcggacct ggacctggac gggattccgg tggccctctt
     8281 cgacaccgcg ggcctccggg aagccccgga cccggtggag gccgaagggg tacgacgttc
     8341 ccgcctcctg gccgaggggg cggacctggt ggtctacctg gtggacggca cccggggcgc
     8401 cgggcccggg gacctggaca ccttgggttc cctgcccggg gctgtccggg tctggaacaa
     8461 ggtcgacctg cccgaggccc tgcccgcccc ggagggctgg atccccctgt ccgcggccac
     8521 gggggagggc ttcccggccc tggcggccga gctgggcgcg cgtctgcggg gcggggcgtt
     8581 gtccggcaac gcgcggggga cagagctcga ccgcggggca tccggagacc tcgacgaagc
     8641 ccaacgggct tcgtcgccgc caatctcggg gcggcccctt ccgcataccg ccccgagatt
     8701 ggcgagcagg cgccaggccg actgcgcccg ccgcgccgcc cgggccctgg ccgccgcggg
     8761 ggccgccctg gccgcgggag cccacctgga cgccgccgcg gtggacctcc gggaagccgc
     8821 ggacgccctg gcggagctca ccggcgagtc cgtgccggaa tcggtgctgg aaatgatctt
     8881 ctcgcgtttc tgcgtgggga aatagcgtcc tcaggaatga accacggagg acacgaagag
     8941 cacggagaac agggctcaaa aaccagggat ccaacaagat ccacaagatc aaaaagatgg
     9001 tacgagggag tccggaggta ctttgaatct tggcttcaca aatttttctt ttccatcttg
     9061 ctgattcttg gatctcggtc tggcttactc cgtgacctcc gtgatctccg tggtatagtc
     9121 cggagaagtt ttaaggaatg tcgatggatt atcaggcaat cgcgcgggac cgggctacgc
     9181 ccgtcccgcg gcaccgaagc ttcgcttcgg cgatccnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnngcgc gggaccgggc tacgcccgtc ccgcggcacc gaagcttcgc
     9361 ttcggcgatc caccgtagag cgctcagtgc tctggagatg ccctgtggat tatgaagcta
     9421 ttgttatagg cggggggcac gcggggatcg aggccgcctc ggcccttgcc cggctgggcc
     9481 accggaccct ccttgtgacc cagaacccgg acgccatcgg ccggctctcc tgcaatcccg
     9541 cggtgggggg cctgtccaaa ggcaacctgg cccgggaagt ggacgccctg ggcggggaga
     9601 tgggccttct gacggacgcc tcgatgatcc agtaccgggt cctgaaccgg tccaggggag
     9661 ccgccgtcca ggccccccgg gcccaggtgg acaagaccct ctactcggcc cgggcccggc
     9721 gccgcctgga gtccctgtcg ggcctggaca tccgcatgga cacggtcacg aacatcctga
     9781 ccgaccggtc cggtacacgg gtcgaggggg tcgtcacaga gcggggagag cggatcgggg
     9841 cggccgtcgt ggtcctgacc acgggcacct ttatggaagg aaggcttttc atcggggaat
     9901 gggaggcttc cgggggccgg ctgggggagg gggcggcgct cggcctgggg acagagctcc
     9961 gggccaaggg ttttacggta ggccgcatga agaccggcac ccccgcccgg gtccggggcc
    10021 ggtccctgga ctacggcctc atggagcgcc aggagggcga gccgccccgt cccttttcct
    10081 tccggaacga cggagtggtc cgcccctcgg tcccctgctg gatcacctac acgaatgagg
    10141 cctcccacca ggccatccgg gcggggctgg accgctcgcc cctgtacggc gggaagatca
    10201 cgggccgggg cccccgctac tgtccctcca tcgaggacaa ggtggtccgc ttccccgatc
    10261 ggacccggca ccaggtcttc gtggagcccg agggcctgga caccgacgag atgtacctga
    10321 acggactttc ctcctccctg cccgaggatg tccaggaagc cttcctccgg accatcccgg
    10381 gtctggagcg ggtcgagatc gtgcggcccg cctacgcggt ggagtacgac tacctggatc
    10441 ctcttcagct ccgtccctcg ctggaaacca agcggatccg gggcctgttc accgcggggc
    10501 agaccaacgg gacctccgga tacgaggaag cctccgccca gggtgtcctc gcggggatca
    10561 acgcctcccg ccgccttcgc ggggaggagc ccctggtcct tccccgttcg gagtcctata
    10621 tcggagtcct ggcggatgac ctgaccaccc tgggcaccca ggaaccctac cgcatgttca
    10681 caagccgcgc cgagcgccgc ctttctctgc gctgcgacac cgcggaccgg aggctcacgc
    10741 ccctgggtcg gcgggagggc ctggtggacg acgcggcttg ggagcgcttc gaggcgcgcc
    10801 gggcggggat cgaagaggcc gccgagctcc tgaggacacg gaaggtcacc gcggcccacg
    10861 cggcgcgggt ggaagctctg tccccggagc aggctacgga gcgagttctg tccccgccgc
    10921 ccccgggcgt tcctcgagct ccgtccccgc tggctcccca cgtcggcgag tcctgggacc
    10981 gggcggtccg ggatccccgg gctttcggcc tggacctggt cgatttcttg cccgaattgt
    11041 cggctctgtc cccggatcag gtcgaaaccg ccgtcctgga cgcccgctac gaaggctacg
    11101 cggcccggga ggcccgtcag gccgcccggc tgggccggtc ggaggaccag cgtatccccg
    11161 agggcttcga ttaccggacc gtggagggtc tttcctccga atcccgggaa aaactcgagg
    11221 ccgtccggcc cctcaccctg ggacaggcgg cccgtatccc gggcatccgg cccgcggacg
    11281 cggccctgct gatggtgaag ctggcaaggg gcgagtgaga gggacggcgc cggttcattc
    11341 cagatccagc accaagcgaa ggccctcctc gacccggcgg agggtggagg gtcggacaag
    11401 gccgactcgg gaatccaggc aggatttatc cagggtgacg acttgggaga tgttcaccac
    11461 cgaatcccgg tccagaccgg actcctcgct ctcgagcagc acgttccccg gcgcctcggc
    11521 cagccggaga ttggacgtga gcacggcgca gatcacggtg gagatccggc tgtcgttgaa
    11581 gctgtccgat tggaggagca ggaccggccg gcggaatccc ggggcggagc ccgaaggcac
    11641 cccgaggtcc gcccagcgga tctcaccacg acggatcggc atcggagaca gccttcctct
    11701 gagccgccgc gagatccgga tccaggggct ccggagagcc cgagtacacc gtgtcgagcc
    11761 gctcgcgtac ggattcccgg gagtgggagc ggatgaactc cgaaagggcc cgggaaaaga
    11821 gccggctccg gggtatgccg agccgctcgg ccgtgcgctc cgcggcctcg tacaggtcgt
    11881 cggggaggga gactgcggtc ttcatatccc tagtataact ccggttatac cgcagaacaa
    11941 ggagtaaccg tgatattcaa catagggaca taatggacgt caacgatttt acgacacgct
    12001 tcgtattttg tacagcgcat ccgtcagcct tctcctctcc tccgccaggt cgtagcggtt
    12061 ctggatgtcc agccagtatt ccggatcgtt tccgaagtac cgggcaaggc gcagggctga
    12121 ttccgcattg atcttcctcc ggccccggag gattccggag acccgggtgg cggggatgcc
    12181 ggagtccttg gcaaggcggt agatcgaaat ttcgcggggg accatgaacc gcttccgcag
    12241 gatctccccg ggggagggga aggtcgatcc ggacatggca agctcctgat agaaattgtt
    12301 atcggctggg ataaccgtag gacggggcgg gcggggcgtc aagagttctt tcaacattcc
    12361 tcagctctgt ccccggcgag gcccacgacg gatcaacctc caaccccggc ttgtccagca
    12421 ggttgaacct gctacgtcgt taacgaggag gagagtgtcg tgaaggttgt aaggatgtgg
    12481 acccgctttg agtgaacggt gtggcggcgc cggatttggt atgcttctta taatacgccc
    12541 gcgatgggac caatttgcag gccataatgc acaatattct tgacaagttg tcacgaacaa
    12601 gatactctct tcccatgaaa actcttccgg taggcgagtt gaagactcaa ttttccgagg
    12661 tcctcgaacg ggtgcggcaa ggggagtcgt tcggtattct ctatggtaaa aggaaaaaac
    12721 ccattgcgtt gataattcct tacactgaaa agacatcaca ggaggagcgc gcgttgggtt
    12781 tcctggaagg caaggtcacc gtgaagttcg cggccgactt cggcatcacc gaggaagagc
    12841 tgctgaatcc ggcatgaagt acctgctgga cacccattcc tttctctggt ccatcgcgga
    12901 ttccggaaaa ctgacgaaaa agagcatcag gatcctcaag aacccgggca atgaagtgtt
    12961 cgtcagttcc gtcaccttgt gggaaatcgc gatcaagaca aggctcggca agctcgagat
    13021 agaaggattt tcgttggatc agttgccggg cattatcgag cacctgaggt tcggccggat
    13081 cgattgtacg atggaagacg cgatcggata tgccggactg aaggaaccga cccacaagga
    13141 cccgttcgac aggatgctaa tcctgcaatg tatacgccga ggcttgacca tgatcagcgc
    13201 cgacgcggag ttcccgaagt tcgtgcccta cgggctcaag ctgatctggt gagaaatccg
    13261 agctctatcc ccgggtcggg tatgcagggc gtggggcggc atccatggcc tcctaatacc
    13321 gggtttccat ctacaacgcc gcggcatcgg gcgaggaagg cttcttgttg gttaccctga
    13381 atcctctccg aaaccctccg agtcccgcaa aatcgacaaa ttccaggaat atgtaaagct
    13441 cgatattccc gatcccctcc ttcggcccct gtttctaagt tctttttgta aaatcattta
    13501 aaaatccatg aaaacccttt gaaatcggaa ttcgcacctt ctcaaggtta caaaaaactt
    13561 ctttttttct tgacacccca ttttgcgaag gctatactgc ctcaaagtga taggggagcg
    13621 ccggaccgta ggttcagccc cctgccaact ccacaaggag gaatttcaga tgaagaagct
    13681 tttcgttctt ctcctcgctc tcgccgtggt taccggcgct ttcgcgcagg tcacgaccag
    13741 catcagtctc cagggccagg tgaaggttct caatgaggac agccagggcc agttcgttcc
    13801 gaagggcgac acctacgata cgttcacctt caaggcgagc acggacacct acggcttcag
    13861 tatgacggac accaactggc tcaccgggct tacggctcct gacggcttcc agggcttcgg
    13921 tgacatccgc gactggaacg tgtggtacaa gctggccgac ggcaaggtga aggtaacctt
    13981 cggtaacctc cgcgacgcca ccttccgcac caccctgacc ggctggctcg ctgatttcgg
    14041 cgccatggac cggattgccg gctacggcct cttcaccacc tggatgccca tggacggtct
    14101 caccgtcggc ctcaacctgc ccttcggcct caccgccgag gacaccgcgg acgtcctgca
    14161 gaaggctgac attggcctgc agtacaagat cgccgacgtc gggactctga acgtcctcgt
    14221 cgatctcaac atggtcggtg atgacaacgt tttcgagctc ggtttcgcct acaccggagt
    14281 tgagaacctc actgccaccg cgatctacaa gggtgagctt gatgccgaga tccactcgtt
    14341 cgtcgtcgaa ggcgactacg taatggacgc tcttgaggct ggcgccagct tcatggggat
    14401 tgccgatgcc tcgaccgatt tcgcctggga agtgaacgcc tacggctact atgacatcac
    14461 cgagaagatc tactccggcc tcgagatcta ctactggagt gacgagacct acgacttctg
    14521 gggtaacctc ggctacaact tcggcaacgg gctcaaggct gatctctccc tcggctacaa
    14581 tgccgatttc tactacgccg tcgccctcaa gtacgccgtt accttctaat ccaaggtagc
    14641 accataccga gtatggaggg gccgacccgc aagggtcggc ctttttttgg agaaaccgaa
    14701 tggttcagat caagcttaaa ccgggattcg tggtacttgc cttgtgtctg gtggggatcg
    14761 tagtctcctg tgcaggatct cccgaggcgg ctgagggaga tgtaattcag gtgtccctgc
    14821 ttacggatag acaaatcagg aatatatatg gaactgagtt gcaagaaaat ccattccttc
    14881 cgcctgcagg gctgttcacc cgatccaaga tgcagttttt tgtattacgg tttgaagggc
    14941 gttccgagta cataaacatc gagaacgtga aagttcgata tggtgatttt tttgccaagt
    15001 ctaaaatatt cggcccggag gatttgaaag cgttttggga agcccgggat acggatacgg
    15061 atgatataaa aagtcaggct ctacttgcaa aaaagtatcg aaccattagt acgtttgtaa
    15121 tagatcccta taagcctttt aggataaaaa ccaatctttc caaggttatg gtgattgttt
    15181 ccgaaatacc ttcggaatcg gaagctgtcc cgatactgga gttggccttc aataatggac
    15241 aagaatcttt ctcaaatgag tatacggggg tcgaattaac tatgtagaga aattcccggg
    15301 gggacggaac gcccccgggg gacagtgctc gccttctgta tagcgcaagc aaaccaaccc
    15361 tacacgggaa ctccccgtgg gacagtgctc gcgcaggtac tccccggggg acagagctta
    15421 ccttctgttt gttgcaagca aagtatccct atccctgcgt atccccggta tgcgtaaacc
    15481 aagggaactt catgacggcg gtctctacca cgtcactgcc cgggcgaacc ggaaagagat
    15541 gatcctggac aaccggggca tgaaggatct cttccttcag gtcgttgccc gggctcggaa
    15601 acggtacgac ttccggatcg agaattttgc ccttatgggg aatcacttcc acctcatgat
    15661 ccgcccgggc cgcagggaat ccctctcccg cattatgcag tggatcctga gtgtgttcgc
    15721 catggcctat aatcgtatcc atgggtacgt cggccatgtc tggggagaaa ggtttttctc
    15781 gcggatcatc cgtaccttcg cgcaattctt gatggtcttc gagtacatcg accggaatcc
    15841 cgtcgaggca aagcaggtcg accaggtctg ggaatgggag tacggaggat tgtggcatag
    15901 acggacgggc cggcgggatc ccccggggac agagctctca ttggtgaaag cccgccttga
    15961 ctccccccgc cccgtcccct aggatacttg caggagcgtt gccgtggcga accgagcgaa
    16021 cgactggctg aggcaggcgg aagaggaact cctctgggca aaggacgctt tggtttcggg
    16081 ccgttgggcc ttggtctgct ttacctctca acaggttgcg gaaaaaagcc tcaagggtat
    16141 ggcgatacgg cggggcgccg cgcaaatcaa gagtcattcg gtcaaggata tagcggcggc
    16201 cctggagatc gacggtgaac tggacgagat gggccgggtg ctggaccaat actacatcac
    16261 gacccggtat ccggatgcct tttattccgg ttcgcccttc gagtatttca cgaagggtca
    16321 ggccgccgaa gccctccgct acgcccaggc cttcctagac cgggcccggg cggaaatccg
    16381 ggggccggac gccgcgacat gaacccggac gaaccggtca tcgtctggaa cacccgggat
    16441 cccctgggcg gccttacgcc ggcccaattc gaggcggagc tccggaaccg gctggccggc
    16501 cgggtggagg cggcctacct cttcggttcc tacggcaccc cggagttcga ccgggactcg
    16561 gatgtggacc tcttcctggt ggtccggacc gccaagccct tcctggaccg tttcctggac
    16621 ttcacggata tccaggacct cgtccccgcc atggacctcc tggtctacac ccccgacgag
    16681 ttcgagtccc tgacctcgga cccttccccg ggcttctgga ccagcgccgt cgcgagcctg
    16741 cggcgggtgc tttagggctt tttaccgctt cttcaccggg gaccgctcga agccggagag
    16801 cttcaggctt ggatccaggt tggggttcca ggatccgtcg tcggtgtaga tgacctcccc
    16861 gccgggtcct acccagcggt gtttccactc gttcgaaccg agctccactt ccttggtccg
    16921 gggattcacg tagagctcct gggaggtgag ggtcaggtac tggtcctgct ggatggcggc
    16981 catggtctgg gaccggctct tggcgatctc ggcgtccacc cgggagatgt cccggagggt
    17041 ggccgcggcg atgtccgccc ggcgagcctg gccggccatc tcgccctgga gccacagggg
    17101 gttgagggcg aaggagttga cgacggtccg ggccacgggg ccccagacct cgaactcggc
    17161 ctccggcgcc cgggcgacga tggtgaaggg gttgtcccag agcccggcgc ccatcatcgc
    17221 gtagccttcc accgcggcga agaggatctc cttgaaccgg acgccgcctt cctcgtaggt
    17281 gaccgtgagg gtcgcggcgt cggcgtggaa ggctccgccc tggctgcgga ttgcctgggc
    17341 cgcgggcatc tggtagaccg cggcgacgat gtccggtcgc tgggcggttt ccaccacctt
    17401 cacgttccgg gcctggggat ggagggcggg gaagagcatc ctgcccagga agtccgcggc
    17461 ccggggatag gccacgacgg gcatcccgtt ccagttgccc cccagcatgg cgttggacgg
    17521 cgagggctgg gcgtagttga tcttgggaat ccagcggatc tggaccttcc cggtggcgtc
    17581 cttggccacg gcgaagtcga tcttggcctc cgtcgcgttc ccgactccgc cctgggcggc
    17641 cagggggttc acccggacca tgcccccttg ggtgatccag cccttgggca ccaggacgaa
    17701 gaaagcgccc tcggtcttgt cgaggtactt ggtgaacacc aggggagggg aggccgccgc
    17761 ggccgggagg gccgcggcga tcaggagcag ggacagagct gagaagaaag ccaaaaaccg
    17821 aaccggtact cgatcgaagc tcctcacggc gccctccctt ccctcgatgg tactgttttt
    17881 accgctaccc cgcgatcagc cccgccagct tctcgaccgt aaggccgaga tgggcggcca
    17941 ccttgtcccc ggggccggag gccccgaagc ggtcgatgga aaggacgtct ttcggggtcg
    18001 tggtcagggc gtcccagccc caggcggtcc cggcctccac ggccacgacc cgggctccgg
    18061 tgggcatgag caccctgagg accgccggat ccgccgctcg caggagctcc aggctcggca
    18121 ccgagaggac ccgtacgtcc cggccgaccg ccttgtccgc ggcggccagg gccaggggaa
    18181 cctcgctgcc cgaggccagg acgaccacgt ccggctcggc cttggagttg cggaccacgt
    18241 aggccccgag tttcatggtc tcccgccagt cgggatcggc cttggccagg acggggaggt
    18301 tctgccgggt cagggccagg acggtggggc cgtcggtccg ttccagggcc aggcgccagg
    18361 cctcggcggt ctcctcggga tccgcgggcc taaggacccg gacgttcggg atgcagcgca
    18421 gggaggccag gtgctcgatc ggttggtggg tcggcccgtc ctcgcccacg aagatggagt
    18481 cgtgggtcag gatgtagatc accgggagct tcatcagagc cgccaggcgc agggaggggc
    18541 ggaggtagtc cgcgaagacc aggaaggtgg cgcagaaggg gcgaaggccc ccgtggaggg
    18601 ccagcccgtt ggacaccgcg gccatggcgt gctcccggac tccgtagtgg atcatccggc
    18661 ccgcggggtt ggcgcgggag aagactccgc cctcgggaag ctggctggag ttggggcccg
    18721 tgaggtccgc ggaccctccc acgaggttgg gccaggcggc ggccgcggcg gccagggcct
    18781 tgccggaggc ggcccgggtg gccaggctct ccccggcggc gaaggcgggg gccgcgaagg
    18841 gggccgaggc cttgccggcc aggaaggcgt ccagttcctt ggccttgccg gggttggcgg
    18901 ccttccaggc ggcgaacagg gcgttccagg cttcccgggt cttccggaag gcggtccggc
    18961 ggccctcgaa ataggagacc gcttcggggg ccacgtagaa gtccgcgtcc gcggggacgc
    19021 ccatcgcctc ccgggcccgc ttgatctcct cggctcccag gggcgccccg tggaccttgt
    19081 gggagccctg catcgtgggg gcgcccttgc cgatcacgct cttcaggatg atgagctttg
    19141 gccgctcgag gtcggtcttg gcttcggcga ccaggcggga gacgcccgcg aagtcgtaca
    19201 tgtccccgga aaggaccttc cagccgtagg cctggtaacg gcgggcgacg tcctcggtga
    19261 acgaaagctc tgtccccccg tcgatggtga tcttgttgga atcgtagaac acgacgagct
    19321 tccccaggcc caggtggccc gccagggagg aagcttcggc ggacaccccc tcctggaggc
    19381 agccgtcccc ggccagggcg taggtccagt ggtcgatcac cgggtgggcg ggggtgttga
    19441 accgggcggc catcatgcgt tcggccatgg ccatgcccac cgcgacggcg atcccctgac
    19501 ccagggggcc cgtggtggtc tcgacgcccg ggcagtggcc gtactcggga tgccccgcgc
    19561 agggggagcc gatctggcgg aaggcgcgga tgtcgtccag gctgaccttg taaccggcca
    19621 ggtgcaggag ggagtagagg aacatggagc cgtgccccgc ggacaggacg aagcggtccc
    19681 ggtccagcca ggcggggtcc gaggggtcgt gcttgaggat ctccccgtag aggagggcgc
    19741 ccagctcggc gcagccgagg gggaggcccg ggtggccgga attggccttc tggatggcgt
    19801 ccatggacag ggtccggacg gtcagggcta ctttcgaaag ggcggcggta tccatgcggg
    19861 actccttatc tatatagagc ttcggccgcg gcgccgatcg ggccgtggac tcggaacgat
    19921 tgtacgggtc ccggcgggaa tgggcaaggt ctccccggga ggcgcggggc gtgccgctcc
    19981 gtccggcggg ggcgcgtcgc gcgggtcagg cggaaggatc cgggtccggc cggccggccc
    20041 cgcccgccgg gaaggcgaag gcacggaagg cccgggcgtc caggggcttg gagaccaggt
    20101 agccctggat ctcgtcgcag ccccgcatcc gcagggcttc cagctgttcc gcggtctcga
    20161 ctccctcggc cacggagatc atctcgaagg accgcgccag ggctatgacc gcggtggcga
    20221 tctgggcccc ctcgtagcgg tccgtgatgg gatcgatgaa ggccttgtcg atcttgagga
    20281 cgtccacggg caactggtcc aaataccgca gggaggagaa gccgatgccg aagtcgtcca
    20341 ccgcgaagcg gacgcccatg tcccggatct cgttcatcac ggtgaccgtc
//
LOCUS       DAOTEI010000046        20182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1756_length_20182_cov_5.092314, whole genome shotgun sequence.
ACCESSION   DAOTEI010000046 DAOTEI010000000
VERSION     DAOTEI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 20182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20182
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_20182_cov_5.092314"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="P5313_06420"
     CDS             complement(<1..667)
                     /locus_tag="P5313_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HRY80029.1"
                     /translation="MDTRLLEERSRILTETRAFFLGRGYLETDTPALAPALIPESCLE
                     VFRTEYDNPFLGRMDLWLVPSPEVWMKPLIARLRRSVFQVCKCFRNSESLGRLHNPEF
                     TMLEYYTVGADSGDSRQLTEELFRRCAGPDTPAWARPPFRRMTMAEAFRELAGLDLEA
                     LQDRGALVEAGRGKGLLVPLEASWEDAFNQVFLSLVEPSLPGDAPLVLERYPAQIECL
                     AADI"
     gene            complement(697..1188)
                     /locus_tag="P5313_06425"
     CDS             complement(697..1188)
                     /locus_tag="P5313_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY80030.1"
                     /translation="MEGTTANQYLTFTLEGEQYAIGVAKVREVLEHTKITKLPKTAEY
                     MKGLINLRGMGVPVIDLRLKFGMPETPVTKDTSIIVMEVEGPEGPVVVGALADAVHEV
                     VEIEAGSVEPAPKFGTRLATEFIRGVGKRDEGFVILLDIDRIFNSEDMEVLRAAEPAA
                     AGV"
     gene            complement(1237..2220)
                     /locus_tag="P5313_06430"
     CDS             complement(1237..2220)
                     /locus_tag="P5313_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HRY80031.1"
                     /translation="MNAPGGPDSALTRVLADTLVTRGVLSFGFLDGIRLAGALEGVPA
                     AVRGSYGLDSARGALAAALHYDTTGSPSGSLDEEYFRDRDGRPLAALGWFARAHWYRE
                     LSDILAAAGRAAGLAAAGTRNSFRIAVNSRLPEKPLALAAGLGPVGRNTLILERRAGP
                     GCVLGVLILPFEPDPSAGTLSKHPGSPASGEPGSECGGCEECVRACPTGAVRREGGID
                     RSLCLQHWAGAEGPVPPAIAEAWRGILYGCDLCLAACPRFRPGVVREASAGPLGRGLE
                     PEAILAARDPDLKSRFRGTALGLSWLGPSLIRRNARLARDSRGKLHGSGQV"
     gene            complement(2240..3472)
                     /locus_tag="P5313_06435"
     CDS             complement(2240..3472)
                     /locus_tag="P5313_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY80032.1"
                     /translation="MESPLQLTFVSFKKDSYIVVEGKQSADRFYIIRQGKVRVSKDVE
                     VVEEEGGNIYGPGDFFGVVSTMSNHSHIETAQAVTDCILISVAREQYGTLIEKNTPVA
                     MKIIMAFSKRMRYLDEALTRLTLKNTAEADPSHLFRVAEYYAKQSQYNQAFYAYHQYL
                     KFCPNGPHVAVARERMGKIAPYSKAVYLDGSNEEFNRFYPKDTMIFSEGMTGSELYII
                     QKGSVKITKIVDNNEVLLAVLKTGDIFGEMAILENKPRSASAIAFEDAYLLAVNKANF
                     ERMVQTQPQIVTRLTTLLAERIWFIYKQLANTLIADPLGRMYDALLIQLEKNRVPLKP
                     GASYVFDFGPKELINMVGLPLGEGNMVMHKLLENSKVKVADNKIQLSDVTEVEKQTEY
                     YRKMQKIEKARKAAQALR"
     gene            complement(3618..4910)
                     /gene="glyA"
                     /locus_tag="P5313_06440"
     CDS             complement(3618..4910)
                     /gene="glyA"
                     /locus_tag="P5313_06440"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRY80033.1"
                     /translation="MSHIESYDPELWKAMSLERRRQREKLELIASENYTSPAVMEAVG
                     SVLTNKYAEGYPGKRYYGGCEFVDMAEDLARDRAKVLFGADHANVQPHSGSQANMGVL
                     YAALNHGDVILGMDLAHGGHLTHGAPVSFSGRQYKVVRYGVNPDTETIDYDELARIAR
                     ESKPRLIIAGASAYSRVLDFQKFRAVADEVGAFLMVDMAHIAGLVAAGYHPSPVALAD
                     FTTTTTHKTLRGPRGGMILIGKDRENTIGAVAPKSGRPKQWSEVLDGMVMPGIQGGPL
                     MHVIAGKAVAFKEALEPSFKDYIRQVVANAKVLASSLAEGGARIVSGGTDNHLMLVDL
                     SPLGITGKEAETWLDAANITVNKNGIPFDQKSPFITSGIRVGSPAVTTRGMKEAEMRQ
                     IAGFIVEVLKSKGDEKVLAAVAEKVLGLTRRFPVPAEA"
     gene            complement(4959..6644)
                     /locus_tag="P5313_06445"
     CDS             complement(4959..6644)
                     /locus_tag="P5313_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRY80034.1"
                     /translation="MSKPSPAGLDRKRGARLVTLALAAVLAAGAGVALKLLLAERSRP
                     VIVALDPSIGEPGGVVRVVGRNFGQERGESRVEIAGVAPTASSYLSWNDRLVELRVPV
                     TVSSGLVYVQTRSGRSRPRLFMNRDRLPLRPQGLLASKSGPAIEGLSAAQGTVGSLLT
                     ISGLNFGANRGEGRVEFAWTGDSGFSNPGPEQVSAFARAEELSLAYESWTDKEIRVRV
                     PDGAASGGVRVLTEKGASDAVFFRVEGGPGTKVFHDRRTYAVSYFVSLTKVKASGENE
                     LYLWIPRPVESASQRGVRVLSQSHVPIVPDYRGVAIYRLRDLRTDVPVTVSQGYIAQV
                     YAVSTDVRADAVRLPPEPAPALYARYTAPDGRIPSGDPTLAAAARKAIGRERNPHRIA
                     EALYSMLLKEVKYTADHVHEAPLDAWKDKTADAYSMAMLYCALLRAGGVPALPVSGFL
                     IGNDRRVFRHFWTEYYLYGLGWVPVDPVLGSGAQIPGFDAPIEDRAKYFGNLDNRRLA
                     ISRGVVELAPMTPGGRTAVKERQYALQDLYEEASGKLEAYSSFWSDLEVTGVY"
     gene            complement(6641..7162)
                     /locus_tag="P5313_06450"
     CDS             complement(6641..7162)
                     /locus_tag="P5313_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80035.1"
                     /translation="MTLPRSRPAWGAFPGMAMLALSVAVSSVHAQSTPGIPEALRDKA
                     LTVTVHTQVPESPTAPAWEAREVKSTLPGSAVSVKLVGESLAVLIHVTPYQGPEGLML
                     VTQAQVWIKDKDSVHYHTTLNTLKVAFGEPVAFYPLGEGKGKAPLRVVIVVQPYQMPA
                     PPAAQASEAPKTP"
     gene            complement(7159..7713)
                     /locus_tag="P5313_06455"
     CDS             complement(7159..7713)
                     /locus_tag="P5313_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRY80036.1"
                     /translation="MCPDRQILSAFADGEVPSPWNEKVLKHLESCEPCSRIVREYRSV
                     SDGLSETKGPDPDAAREAVWSRLVETSRRRRFDPLWRARFAVPLPVAAAAMLAFAVLS
                     TLVVTSRRENSALRMAVFAASELTPAPNQATAMESVLQFLESQDSRITVTVQLPPGAG
                     LESFGKPVFMKAPDLAPMILEGGK"
     gene            complement(7703..8248)
                     /locus_tag="P5313_06460"
     CDS             complement(7703..8248)
                     /locus_tag="P5313_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRY80037.1"
                     /translation="MSGVLFNREEGFGPERIRALYDAAFTLLYRIAYRITGSEEAAQD
                     VVHDSFERLMQKSIPFPSQDDAKYWLIRVVKNNALNWAKRKGREARAYERYFRESDEE
                     SSSAEDETLRRFTAEEVKEKLEMLPENLRMVLVLKEYGLLNYKEIGRVLGISEGNVKV
                     RVFRAREALLSLFREGNRDVS"
     gene            complement(8265..9695)
                     /gene="cysS"
                     /locus_tag="P5313_06465"
     CDS             complement(8265..9695)
                     /gene="cysS"
                     /locus_tag="P5313_06465"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRY80038.1"
                     /translation="MHPIRLFNTLGRELQEFQPVHPGKVGFYGCGPTVYNFAHIGNLR
                     MYVFEDTLARALRYLGYEVRHVMNITDVGHLTGDDDSGDDKMVKSARERGQSVLEIAR
                     FYTDAFFRDTGLLNIRRPDVVCKATEHVADMIDLIRRIEANGYTYFAGGNLYFDVSRF
                     PGYGRLALLNLEDLKAGARIEVDANKRNPYDFVLWFTKGKFENQALVWDSPWGSGYPG
                     WHIECSAMSMKYLGESIDIHAGGIDHIPIHHTNEIAQSESATGKPWVKYWLHGEFLVM
                     GKGKMSKSSGSFLTLRSLTDAGYEPLDYRYFCLGGHYRSQLQFSFEALDSARASRRSL
                     GERLRTLRSQAEPAPAVPADGKARVWLDAFADHLAEDLALPRALADLWGLLRDPEVTP
                     PEALAGAYDMDRVLGLGLREAAEAAPEEKAASPERAAQIERRIAERAAAKKAKDWARA
                     DAIRDELKAEGVLLTDGPSGTTWKLS"
     gene            9768..10691
                     /gene="murB"
                     /locus_tag="P5313_06470"
     CDS             9768..10691
                     /gene="murB"
                     /locus_tag="P5313_06470"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRY80039.1"
                     /translation="MPTVREFLEKIKFDGPVSYGEPMSGRTTFRIGGPADALAEPRTR
                     ESLAALLSAARSEGLPVFLLGGGANLLVRDGGIRGLVVSTRGLAGLRITEAGIEAEAG
                     VPVTDLVGAAAEAGLSGLEFAAGLPGSVGGAVYMNARCYEREFADLMTRVDYLDSRGN
                     LGSLVPAKGEWAYKSTPFMPGGLQSGSVVLGAEFRLRPGEKPAILSRMRELEADRAAK
                     GHFDFPCAGSVFKNDRRFGRPTGRILDELGFRGRALGTAAVSEKHANIFVNPGSATAA
                     DMLALIEQARREARERYGYELETEVVIVGEP"
     gene            10853..13429
                     /locus_tag="P5313_06475"
     CDS             10853..13429
                     /locus_tag="P5313_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY80040.1"
                     /translation="MDLTLPVTDPVGVFFILFAALFAAPILAELVRLPGLVGTVLAGM
                     LLGPHALGLLDAGAFLETLGGFGLLYIFFTAGLELDLRKLRRKSADAVVFGGLTFLIP
                     LGLGYAAGILVFGLPVVPAVLFGSLFASHTMLPYPIVQAMGLSGSRAVYASVGATTIT
                     DILAMLALALTTAGSGPGWEFWLKTLGILTAWTVGAALLLPPLISRFFRRIKSGGSSE
                     FLFALAVAFFCSAAAALAGIEPAVGAFAAGILLNRHIPERSGLMSLLKFAGDALFIPL
                     CVLYIGVLTDARALLTDGRMAALAAVMLGVVLVSKYASSLFLKPLFGFSLGEVNISFG
                     LSVNQTASTLAAALVGYRLGLLGEAVLNGTVVMFIVTCALGPFVTRKAASALAVETTR
                     GQAGPGLTGERILIAVSNPGTLRPLTDFAFLMRDPKSPEPVLPVCVVQDSPNADRDLE
                     QAETVLAQAIAQGVAEGVPVKPSTRVSLNAAEGILQAAEDQEATTVLVGWNRPPRPSG
                     AIFGDVIDQVVAGGRHLTVVARLRRPLPESPSLVLVVPPFAERNPGAARAYALMRRIL
                     GGVPAKLTVLTLASDSSGVRDSLRRIRGAAPHIQELESWKEFSGAIAKQPEGTAFLFL
                     CPRPGRLAWHPYLEQLPRDAAEAFPDSTLMVTYLPDEGGGAENYPAAAAESVSGPAGE
                     AAGPAAEGTTPAPAPAAEGIPEPAAPPDSSDLFYRTLAGSGRVIPDLPAAAVVDAVRI
                     LLERGFPGDRRSVNRLTGIYAEIAQKQPIELAPGVLLLHAHVDNIEEPRILIGAKPAG
                     LRLLALEEPIRIVVLLCAPSSQSPEDHLRTLSLIAQALKRPERVARILAAKTEEDLRE
                     DS"
     gene            complement(13505..15289)
                     /locus_tag="P5313_06480"
     CDS             complement(13505..15289)
                     /locus_tag="P5313_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRY80041.1"
                     /translation="MHELDGHQIYRVFRAGARRVLGVRKELDRINVFPVPDGDTGTNL
                     AATLASAVESSRPTDSAGKTMENIADAALAGARGNSGVIFAQFISGFYEALTDAATIS
                     MEKFVAAVENAYSRARSAISNPRDGTILSLIGDWAAALRAELGRARNFRELLGGTLPA
                     LHSSLADTPNRLAELKAAGVVDAGASGFVAFVAGAHSYVEGGEDADEALDISDLDPGG
                     EAPDLHDLSELRHRYCTEALVVGRDLDPESIRSEVSDLGDSLIVAGNRRKLRIHIHTD
                     RPADLIERLSARGDVLQQKVDDMKEQFLTAHVRKYPIAVVTDSGCDLPAALLEEYQVH
                     VVPLHIRVGEVEYLDRRTLSPERFYELADRAPSFPKSSQPSGAVFLRTYRFLAERYDS
                     IIAVHLAGKLSGTLEASRRAAEQVAAETGKRIDVVDSRHLSGSLGLVVLRTARAVAEG
                     RSHDRILADLPAWSDKADILVSVRTLRYMVRGGRVSPLQGLAAKILNLKPIVSLERDG
                     SSKLYGKAFSVEANLKRILDMTLAKHRSGKLRNYAVVHAHDPEGAQDLVRRLEAALGF
                     PPEYVMEISSVIALNAGRGALAVVSMAE"
     gene            complement(15474..19637)
                     /locus_tag="P5313_06485"
     CDS             complement(15474..19637)
                     /locus_tag="P5313_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRY80042.1"
                     /translation="MKTFRPAFPAILFAALAVLSAPAYDPPRGGEYAQLLTSPFLLAG
                     GSWAASTESPASDYLNPAASAGQQRVVLDANYAAVVGMDAQTGWGSALNLGLTIPKPY
                     GVWTASAQFLSIPDSLTSLPLGTAFRVEGSIAKDLYPNLYAGIGAGLVIGDDWGLAAD
                     LGILRKVGDFLFFKNFQWGAVLSGLGKPYVPAGLGAAGGAATAFPGPYTLTLGGRGDF
                     IQTEKFRAGASLDLAAPSFQNLLVNAGLEGSFREKVILRLGWGLNVHEILNSPAALLP
                     SLGLFGKFELNRKSDESFISKQGWDRSEIRPGLAVKPLYDGIYAYSAGINIPLGVVDR
                     EAPVVQLTYPETPWGAYYLSPNSDGKNDQIELPLSIKERRYLSGYTMTVYRGDPAPAG
                     SAAPASPEAAPAVPAEGSAVRVIGNKESRPETSGLAGFRDRLAYVKKGIPVPDKLVWN
                     GFADDGKPVPDGRYTIVVEAVDDNGNKGTSKVHEVFVDSTPPRVEIAAPRDAVDLIFS
                     PDGDGGKDTLTIRKTGSQEDLWTARVLDASGASVKTVEYKASAPADLTWDGRNDAGTV
                     VPDGVYSFVVESTDRAGNTVSRRLDNIVVNTQQPPITVAIDVSAFSPNGDGVRDTVAL
                     LPGVPIRTGLASWRLAVLDEGRTERWSRTGSDAASLPERWAFDGKDAAGKPLPEGSYR
                     AFLEVRYVNGYAPSATSPLFLLDVTPPSARASVERPTFNPAGAAGQNAAALSASGSRE
                     LAWTAEVTDAAGKAVRTWSFPGEPDARIEWDGRDESGKIVPDGAYAFRLRSVDRAGNS
                     GSSAPVNLRVDTEKKAAVVSSDARAFSPNGDGVKDSVRIVPEVRSSDAPASWDVVVKS
                     SAGGAAVRSWKGTGAVPRELVWNGRNDGGTQAPDGSYEAELTVRFANLDTAVATSGVI
                     ILDTVAPKAQVSAAPLLFSPNGDGRKDEVVITQSAAPGDDWRGSILAANGRPVRTWTW
                     RGALVPVSWDGRDDEGNPASDGRYSYVLSSEDAAGNKAEYRIDGIAADTRAVQVFVTA
                     NRAGFSPNGDGRYDDILFSPIVNLRDGVESWNLALADAAGTARKTYSGANIASLPREL
                     VWDGKDDRGNPAPQGTYTAVFTVVYAKGDAPQARTASFVLDTEGPRVRLRTSPELFSP
                     DNDGVDDELTVAIAIDDASPIETWQFEIFESSVEEGAGPAKVRLFTSWSGTGNPTERI
                     LWDGRSNKGELVEGASDYPYAMAVMDAWGNSAKVEGKVTVDVLVIRDGDRLKIKVPSI
                     VFRSNGADFDGLPKEKTDRNEVVLRRIAQILNRFRDYKIRIEGHANSIGKIYGYSAAQ
                     VNKEETEELIPLSTNRAEAVRRLLVQFGVDARRLSVRGLGSSEPVVDFKDAENRWKNR
                     RVEFILIRE"
     gene            complement(19846..>20182)
                     /locus_tag="P5313_06490"
     CDS             complement(19846..>20182)
                     /locus_tag="P5313_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80043.1"
                     /translation="QELMQAYLTEHGLPGVRILVFHQFTEKMIQDRSRVRADFDRVLP
                     VHVMDGFGPPALKRYSYALNSRAANLPLKGFKLFFKSGYPGAGFDEPLMTPQEVLGLA
                     PAPVLIMYQ"
BASE COUNT         3035 a         6821 c         6953 g         3373 t
ORIGIN      
        1 ggatgtccgc ggccaggcac tctatctgcg ccggataccg ttccaggacc aggggtgcgt
       61 ccccggggag ggagggctcc accaggctca ggaagacctg gttgaaggcg tcctcccagg
      121 aggcctcgag ggggaccagg agtcccttgc cgcgtcctgc ctccaccagg gctccccggt
      181 cctggagggc ttccaggtcc aggcccgcga gctcccgaaa ggcctcggcc atggtcatcc
      241 ggcggaaggg cggacgcgcc caggccggcg tatcgggacc ggcgcagcgc cggaacagct
      301 cctccgtgag ctgtcgggaa tccccggaat ccgccccgac ggtgtagtac tccagcatcg
      361 tgaactccgg gttgtgcagg cggcccaggg actcggaatt gcggaagcat ttgcacacct
      421 ggaagacgct gcgccggagc cgggcgatca ggggcttcat ccagacttcc ggggagggga
      481 ccagccagag gtccatgcga cccaggaagg ggttgtcgta ctcggtgcgg aagacctcca
      541 ggcaggattc cgggatcagg gccggggcga gggccggggt atccgtctcc aggtagccgc
      601 gcccgaggaa gaaggcccgg gtttccgtga ggatcctcga gcgttcctcg agaagtctcg
      661 tgtccatgcg ttccgccccg gagtcccgaa ccgcggctag acgccggcgg cggcgggttc
      721 cgcggcgcgg aggacttcca tgtcctcgga gttgaagatc cggtcgatgt ccaggaggat
      781 gacgaagccc tcgtcccgct tgccgacacc ccgtatgaac tccgtggcca gccgggtccc
      841 gaatttcggg gcgggttcca cgctcccggc ctcgatctcc acgacctcgt ggaccgcgtc
      901 cgccagggcc cccacgacga cgggaccctc cggcccctcc acctccatca cgatgatgct
      961 ggtgtccttg gtgaccgggg tctcgggcat cccgaacttg aggcggaggt ctatgacggg
     1021 aactcccatg ccgcgaaggt tgatgaggcc cttcatgtac tccgcggtct tgggcagttt
     1081 ggtgatcttg gtgtgttcca ggacttcccg gaccttggcg acgccgatgg cgtactgctc
     1141 gccttccagg gtgaaggtca ggtactggtt ggctgtggtt ccttccatga cggttcgcct
     1201 cctaatctta gagtttatcg gtacggaccg gcggagtcaa acctgaccgg acccgtgcag
     1261 ttttccccgg gaatcccggg ccagtcgggc attcctccgg atcaggctcg ggccgagcca
     1321 ggaaagcccc agggcggtcc cccggaaccg ggacttcaga tccggatccc gggccgccag
     1381 gatggcctcg ggctccaggc cccggcccag gggtcccgcg gatgcctccc gcacgacccc
     1441 cggccggaac cgggggcaag cggccaggca gaggtcgcac ccgtacagga tgccccgcca
     1501 ggcttccgcg atcgccgggg gcacgggtcc ctccgccccg gcccagtgct gcaggcatag
     1561 ggatcggtcg atgccccctt ccctgcggac cgccccggtc gggcaggccc ggacgcactc
     1621 ctcgcagccc ccgcactcgg agccgggttc cccggaagcc ggggaacccg gatgcttcga
     1681 cagggttccg gcggaagggt ccggctcgaa gggcaggatg aggaccccca ggacgcagcc
     1741 cggtccggcc cgcctctcca ggatcagggt gttccggccc acgggcccca gccccgcggc
     1801 gagcgccagg ggtttttccg gcagacggga gttcaccgcg atccggaagg agttccgggt
     1861 gccggcggcc gcgagccccg cggcacggcc ggccgcggcc aggatgtccg agagttcccg
     1921 gtaccagtgg gcccgggcga accagcccag ggccgccagg ggccggccgt cccggtcccg
     1981 gaaatattct tcgtccaggg accccgaggg gctgcccgtc gtgtcgtagt gcagggccgc
     2041 ggccagggcg ccccgggcgg agtccaggcc gtaggacccc cggacggcgg ccggcacacc
     2101 ctcgagggcc ccggccaggc ggatgccgtc caggaagccg aaggacagga cgccccgggt
     2161 cacgagggtg tcggcgagga cgcgggtgag ggcgctgtcg ggaccgccgg gggcattcac
     2221 ggccggaggg ggggccgggt cagcggaggg cctgggcggc cttccgggcc ttctcgatct
     2281 tctgcatctt gcggtagtac tcggtctgct tctccacctc ggtcacgtcg gaaagctgga
     2341 tcttgttgtc cgccaccttg accttgctgt tctccaggag cttgtgcatc accatgttgc
     2401 cctccccgag gggcaggccc accatgttga tcagctcctt gggtccgaag tcgaagacgt
     2461 aggaggcccc gggcttcagg ggcacccggt tcttctccag ctggatgagg agggcgtcgt
     2521 acatccggcc caggggatcc gcgatgaggg tgttggccag ctgcttgtag atgaaccaga
     2581 tccgctcggc caggagggtc gtcaggcggg tgacgatctg gggctgggtc tggaccatcc
     2641 gctcgaagtt ggccttgttg accgccagga ggtaggcgtc ctcgaaggcg atggcgctgg
     2701 cgctgcgggg cttgttctcc aggatggcca tctccccgaa gatgtccccg gtcttgagca
     2761 ccgcgaggag gacctcgttg ttgtccacga tcttggtgat cttcacggaa cccttctgga
     2821 tgatgtagag ctccgagccc gtcatcccct cggagaagat catggtgtcc ttggggtaga
     2881 agcggttgaa ctcctcgttg gacccgtcca ggtagaccgc cttggagtag ggggcgatct
     2941 tgcccatccg ctcccgggcg accgccacgt ggggaccgtt cgggcagaac ttgaggtact
     3001 ggtggtaggc gtagaaggcc tggttgtact ggctctgctt ggcgtagtac tccgccaccc
     3061 ggaagagatg ggaggggtcc gcctccgccg tattcttgag ggtcaggcgg gtcagggcct
     3121 cgtccaggta gcgcatccgc ttggagaagg ccatgatgat cttcatggcc acgggggtgt
     3181 tcttctcgat gagggtcccg tactgttccc gggccacgga gatgaggatg cagtcggtca
     3241 ccgcctgggc ggtctcgatg tggctgtggt tggacatggt ggacacgacg ccgaagaagt
     3301 cgccggggcc gtagatgttg cccccctcct cctccaccac ctccacgtcc ttggacaccc
     3361 ggaccttgcc ctgacggatg atgtagaagc ggtcggcgct ctgcttgcct tccacaacga
     3421 tgtatgagtc ttttttaaaa cttacgaatg tcagctggag aggactttcc atcccgcctt
     3481 ccgtatgact gagctgtctg ggctaaagta tagatacccc cgaatgacct gtcaattgcg
     3541 cgaggtgccc gggcgcaagc gcccggacac ctcgcggcaa cgaagccctg cgggcttcgt
     3601 cgaatttcag gtacgggtca ggcctccgcg gggacaggga agcgcctggt caagcccagg
     3661 actttctccg cgacggccgc caggaccttc tcgtccccct tggacttgag gacctccacg
     3721 atgaacccgg cgatctggcg catctcggcc tccttcatgc cccgggtggt caccgcgggg
     3781 ctgcccaccc ggatgcccga ggtgatgaag gggctcttct ggtcgaaggg gatcccgttc
     3841 ttgttgaccg tgatgttcgc ggcgtccagc caggtctccg cctccttgcc cgtgatgccc
     3901 aggggtgaga ggtccaccag catgaggtgg ttgtccgtgc cgccggacac gatccgggcc
     3961 ccgccctccg cgagggagga ggccaggacc ttggcgttgg ccaccacctg gcggatgtag
     4021 tccttgaagg agggctccaa agcttctttg aacgcgacgg ccttgcccgc gatcacgtgc
     4081 atgagggggc cgccctggat gcccggcatg accatcccgt ccaggacctc ggaccactgc
     4141 ttgggccggc cgctcttggg agccacggcc ccgatggtgt tctcccggtc cttgccgatc
     4201 aggatcatcc cgccccgggg accccggagg gtcttgtggg tggtggtggt ggtgaagtcc
     4261 gccagggcta ccggggacgg atggtacccc gcggccacga ggccggcgat gtgggccatg
     4321 tccaccatca ggaaggcccc gacctcgtcc gccacggccc ggaatttctg gaagtccagg
     4381 acccgggagt aggcggaggc gccggcgatg atgagccggg gcttggattc ccgggcgatc
     4441 cgggccagct cgtcgtagtc gatggtctcg gtgtccgggt tcaccccgta ccggaccacc
     4501 ttgtactgcc ggcccgagaa ggagaccggg gccccgtggg tcaggtggcc gccgtgggcc
     4561 aggtccatgc ccaggatcac gtccccgtgg ttcaaggcgg cgtagaggac ccccatgttg
     4621 gcctggctgc cggaatgggg ctggacgttg gcgtggtccg ccccgaagag gaccttggcc
     4681 cgatcccggg cgaggtcctc ggccatgtcc acgaactcgc agccgccgta ataacgcttg
     4741 ccggggtagc cctcggcgta cttgttggtc agcaccgaac cgacggcctc catcacggcg
     4801 gggctcgtgt agttctcgct ggcgatgagc tccagctttt ccctctggcg ccttcgctcc
     4861 aggctcatgg ccttccaaag ctcgggatcg tacgactcga tatgggacat gtgcactcct
     4921 tttttaacgg gaccgcgccg ccccggatct cgccgggatc agtacacccc cgtgacttcc
     4981 aaatcgctcc agaacgagct gtaggcctcc agcttgccgg acgcctcctc gtacaggtcc
     5041 tggagggcgt actgcctctc cttgaccgcc gtgcgtccgc cgggggtcat gggcgccagc
     5101 tcgacgaccc cccgcgatat cgccagcctc cggttgtcca ggttcccgaa gtacttggcc
     5161 ctgtcctcga tgggcgcgtc gaagcccggt atctgggccc cggatcccag aaccggatcc
     5221 acgggcaccc agcccaggcc gtacaggtag tactccgtcc agaaatgccg gaaaaccctc
     5281 cggtcgttcc cgatcaggaa tccggatacg ggcagggcgg gcacgccccc ggcacggagc
     5341 agggcgcagt acagcatggc catggagtag gcgtccgcgg tcttgtcctt ccaggcgtcc
     5401 aggggggcct cgtggacatg gtcggcggtg tacttcactt ccttaaggag catagaatac
     5461 aacgcttccg cgatcctgtg gggatttctt tcgcgcccga tcgccttccg ggccgccgcg
     5521 gccagggtcg gatccccgga cgggatccgt ccgtccgggg cggtgtaccg ggcgtagaga
     5581 gccggggcgg gttccggagg caaccgtact gcgtccgccc ggacgtccgt gctcacggca
     5641 tagacctggg cgatgtaccc ttgggacacc gtcaccggga cgtccgtccg aaggtcccgg
     5701 aggcggtaga tggccacccc tcggtaatcg ggaactatgg gcacgtggga ctgggagagg
     5761 acccggaccc cccgctggct ggcggattcc acgggccggg ggatccagag gtagagctcg
     5821 ttctcccccg aagccttcac cttggtcagg ctcacgaagt aggagaccgc gtaggtcctc
     5881 cggtcgtgga agaccttggt ccccggcccc ccctccaccc ggaagaagac ggcgtcgctg
     5941 gctccctttt ccgtcaggac ccggaccccg ccggaggcgg ccccgtccgg gacccgaacc
     6001 cggatctcct tgtcggtcca ggattcgtag gccagggaga gctcctccgc ccgggcgaag
     6061 gcggaaacct gctcgggtcc cggattggag aagcccgaat ccccggtcca ggcgaattcc
     6121 accctgccct cgccccggtt ggccccgaag ttgaggcccg agatcgtgag cagggagccc
     6181 acggtcccct gggccgcgga cagtccctcg atcgcgggac cggacttgga cgccaggagc
     6241 ccctgggggc ggagaggaag ccggtcccgg ttcatgaaca gccgggggcg gctgcgaccg
     6301 ctccgggtct gcacgtacac gagcccggag ctcacggtca ccggcacccg cagttcgacc
     6361 agccgatcgt tccaggacag gtaggaggag gccgtgggcg ccacccccgc gatctcgacc
     6421 cggctctccc cccgctcctg cccgaagttg cgccccacga cccgcaccac gccgccgggc
     6481 tccccgatcg agggatccag agcgacgatc acgggccggg accgctccgc gagcaggagt
     6541 ttcagggcga cgccggcccc cgcggccagg acggccgcca gggcaagggt taccaggcgc
     6601 gcgccccgct tgcggtcgag gccggccggg gagggcttgc tcatggagtc ttgggagctt
     6661 cggaagcctg ggccgcgggg ggggcgggca tctgatacgg ctggacgacg atgaccaccc
     6721 tcaagggggc cttccccttg ccctccccga ggggatagaa ggccacgggc tccccgaagg
     6781 ctactttgag ggtattgagg gtcgtatggt agtggacgga atccttgtcc ttgatccaga
     6841 cctgggcctg ggtcaccagc atgagcccct ccggcccctg gtagggtgtc acgtggatca
     6901 ggacggccag ggattccccg accagcttga cggacaccgc cgagcccggg agggtcgact
     6961 tcacttcccg ggcttcccat gcgggcgccg tggggctctc ggggacctgg gtgtgcaccg
     7021 tcaccgtgag ggccttgtcc cggagggctt ccgggatgcc cggggtcgat tgggcgtgca
     7081 cggaggagac ggctacgctc agggccagca tcgccatgcc cgggaaggcg ccccaggccg
     7141 gccgggaccg gggaagggtc attttccgcc ctctaggatc atgggagcca ggtcgggggc
     7201 cttcatgaag accggtttgc cgaaggattc caggcccgcc cccgggggaa gctggacggt
     7261 gacggtgatg cgggaatcct gggactccag gaattgcagc acggattcca tggccgtggc
     7321 ctggttcggc gccggggtca gttcggaggc ggcgaagacg gccatccgga gggcggagtt
     7381 ctcgcgccgg gaggtgacga ccagggtgga aaggaccgcg aaggccagca tcgcggcggc
     7441 ggccacgggg aggggcaccg cgaaccgggc cctccagagg ggatcgaatc ggcggcggcg
     7501 ggaggtttcg accaggcggc tccagacggc ctcccgggcg gcatcgggat cggggccctt
     7561 cgtttcggac agcccgtccg aaacggaccg gtactcccga acgatccggg aacacggttc
     7621 gcaggattca agatgcttca gaaccttctc gttccacggg gacggaacct ctccgtccgc
     7681 gaacgcggag agaatctggc ggtcaggaca catcccggtt accctcccgg aacagggaaa
     7741 ggagcgcttc ccgggcccgg aacacccgga ccttcacgtt cccctcgctg atacccaata
     7801 ccctgccgat ttccttgtag ttcaacagac cgtattcctt cagtacgagc accattcgga
     7861 ggttctccgg cagcatctcg agcttttcct tcacttcctc cgcggtgaac cgccggaggg
     7921 tctcgtcttc ggcggacgag gattcctcgt cggactcccg gaagtaccgc tcgtatgccc
     7981 tggcttcccg ccccttccgc ttggcccaat tgagggcgtt gttcttgacg acccggatga
     8041 gccagtactt ggcgtcatcc tgggaaggga agggaatact cttctgcatc agccgttcga
     8101 aagagtcgtg cactacgtcc tgcgcggcct cctccgaacc ggtgatgcgg tacgctatcc
     8161 ggtagagcag ggtgaacgcc gcgtcgtaga gcgcgcgaat ccgctccggg ccgaaccctt
     8221 cctccctgtt aaacaaaacc ccgctcacga tcgttacagg gccttcacga gagcttccag
     8281 gtggtgccgg aggggccgtc cgtcaggaga accccctcgg ccttcagttc gtcccggatc
     8341 gcgtccgccc gggcccagtc cttggccttc ttggccgcgg cgcgctccgc gatgcggcgc
     8401 tcgatctggg cggcccgctc gggagaggcc gccttctcct ccggggcggc ttccgcggct
     8461 tcccgcagcc ccaggcccag gacccggtcc atgtcatagg ccccggccag ggcctccggg
     8521 ggggtaactt cggggtcccg gagaagcccc cacaggtccg ccagggcccg gggaagggcc
     8581 aggtcctcgg caaggtgatc cgcgaaggcg tccagccaga cccgggcctt gccgtccgcg
     8641 gggacggccg gcgccggctc ggcctgggac cggagggtcc ggaggcgctc gcccagggag
     8701 cggcgggagg cccgggcgga gtccagggcc tcgaaggaga actggagctg ggaccggtag
     8761 tggccaccca ggcagaagta ccggtagtcc aggggctcgt atcccgcgtc cgtcagggac
     8821 cggagggtca ggaagctccc ggaggacttg gacatcttgc ctttgcccat cacaaggaac
     8881 tccccgtgga gccagtactt gacccagggc ttaccggtgg cggactcgga ctgggctatc
     8941 tcgttggtgt ggtggatggg gatgtggtcg atcccgcccg cgtggatatc gatgctctcg
     9001 cccaggtact tcatgctcat ggcggagcac tcgatgtgcc agcccgggta cccgctgccc
     9061 caggggctgt cccagaccag ggcctggttc tcgaacttgc ccttcgtgaa ccagagcacg
     9121 aagtcgtagg gattgcgctt gttggcgtcc acctcgatcc gggccccggc cttgaggtcc
     9181 tccaggttga ggagggcgag ccggccgtaa ccggggaacc gggacacgtc gaagtagagg
     9241 ttgccccctg cgaaataggt gtacccgttg gcctcgatgc gccggatgag atcgatcatg
     9301 tcggccacat gctcggtcgc cttgcagacc acgtcggggc gccggatgtt gaggagcccg
     9361 gtgtcccgga agaaggcgtc cgtatagaac cgggcgatct ccaggacgct ctggccccgc
     9421 tcccgggcgc tcttcaccat cttgtcgtcc ccggagtcgt cgtccccggt gaggtgcccc
     9481 acgtccgtga tgttcatcac gtgccgaacc tcgtatccca ggtaccggag ggcccgggcc
     9541 agggtgtcct cgaagacgta catgcggagg ttgccgatgt gggcgaagtt gtagaccgtg
     9601 ggcccgcagc cgtagaagcc gacctttccg ggatgtacgg gttggaactc ctggagttcc
     9661 ctgccgaggg tgttgaacag ccggatcggg tgcacgggca agcctcctgg cggcgccggg
     9721 aacggcctcg ttgcgggccg tgaacgattt cctgtacaat tcgggacatg cctacggtac
     9781 gggaattcct ggaaaaaatc aagttcgacg gcccggtgag ctacggcgag cccatgtcgg
     9841 ggcgcacgac cttccggatc gggggccccg cggacgccct cgccgagccc cggacccggg
     9901 aatccctggc cgccctgctc tccgccgccc gctcggaggg cctgcccgtc ttcctcctgg
     9961 ggggcggggc caacctcctg gttcgggacg gagggatccg gggcctcgtg gtctcgaccc
    10021 gggggctggc ggggcttcgg atcacggagg cggggatcga ggcggaggcc ggggtgcccg
    10081 tcacggacct cgtaggcgcg gccgcggagg cgggcctgtc gggcctggag ttcgcggcgg
    10141 gcctgccggg ttccgtggga ggggccgtct acatgaacgc ccggtgctac gagcgggagt
    10201 tcgcggacct catgacccgg gtggactacc tggactctcg ggggaattta ggctccctgg
    10261 tcccggccaa gggcgaatgg gcctacaaga gcaccccctt catgccgggc gggctccagt
    10321 ccggctccgt ggtcctgggc gcggaattcc gcctgagacc cggggaaaaa cccgccatcc
    10381 tctcccggat gcgggaactg gaggcggacc gggccgccaa ggggcacttc gacttcccct
    10441 gcgcaggcag cgtcttcaag aacgaccgcc gcttcggccg gcccacgggc aggatcctgg
    10501 acgagctggg cttccggggc cgcgccttgg gcaccgcggc ggtctcggaa aagcacgcca
    10561 acatcttcgt gaatcccggc tccgccaccg ccgcggacat gctcgccctt attgagcagg
    10621 cccgccggga agcccgggag cggtacggtt acgagctgga gacggaggtg gtcatagtcg
    10681 gtgaaccgtg aacggtgaac ataggacagt ggacgggaag gtgcgaccgt aacccgccgc
    10741 attccgcgcc gatgcgtgga actcggccgc gttcgtgtgc cgcttccgtc cactgaccac
    10801 cgtccaccgt ttacagcttg cgttccccgc tatctcgcat tagcatatac atatggacct
    10861 cacgcttccc gtcaccgacc cggtcggcgt cttttttatt ctcttcgccg ccctcttcgc
    10921 ggcccccatc ctggcggaac tggtccgact gcccggactg gtggggacgg tgctcgcggg
    10981 aatgctcctg ggaccccacg ccctgggact gctcgacgcc ggcgccttcc tggaaaccct
    11041 gggaggcttc ggccttctgt acatcttctt caccgcggga ctggagctcg acctccgcaa
    11101 gcttcgccgg aaatccgccg acgccgtcgt cttcgggggg ctgaccttcc tgatccccct
    11161 cgggctgggg tacgcggcgg ggatcctggt gttcggcctg cccgtggtgc ccgccgtcct
    11221 gttcggaagc ctcttcgcct cccacacgat gctgccctat cccatcgtcc aggcgatggg
    11281 cctgtccgga agccgggcgg tgtacgcctc cgtcggggcc acgacgataa cggacatcct
    11341 ggccatgctg gccctggccc tcacgaccgc cggttccggt ccgggctggg agttctggct
    11401 caagaccctg gggatcctca ccgcctggac cgtcggggcg gccctgctcc tgccgccgct
    11461 catcagccgg ttcttccggc ggatcaagtc cggtgggtct tctgagttcc tcttcgccct
    11521 ggcggtggcc ttcttctgct ccgccgccgc ggccctggcc gggatcgagc cggccgtggg
    11581 agccttcgcc gcgggcatcc tcctgaaccg ccacattccc gagcgcagcg gcctcatgtc
    11641 cctgctcaag ttcgccgggg atgccctgtt catccccctg tgcgtgctgt acataggcgt
    11701 cctcaccgat gcgagggccc tgctcacgga cgggcgcatg gccgccctcg ccgcggtcat
    11761 gctgggggtg gtgctggtct ccaagtacgc ctcctccctc ttcctcaagc ccctgttcgg
    11821 cttctccctg ggggaggtga acatctcctt cgggctttcc gtcaaccaga cggcgtccac
    11881 cctggccgcc gccctggtcg ggtatcggct gggactgctc ggggaggccg tcttgaacgg
    11941 gacggtggtc atgttcatcg tgacctgcgc cctgggaccc ttcgtcaccc gaaaggccgc
    12001 gtccgccctg gcggtggaaa ccacccgggg gcaggcggga cccgggctca cgggggagcg
    12061 gatcctgatc gcggtctcca atcccggcac cctccgcccc ttgacggact tcgcgttcct
    12121 gatgcgggac ccgaagtctc cggagccggt cctgccggtc tgcgtggtcc aggactcgcc
    12181 caacgcggac cgggacctgg agcaggccga gacggtcctg gcccaggcca tcgcccaggg
    12241 cgtggccgag ggagtcccgg tcaagccctc cacccgggtc agcctcaacg cggcggaagg
    12301 catcctgcag gccgccgagg accaggaggc cacgacggtg ctggtgggat ggaaccgccc
    12361 gccccggccg tcgggggcca tcttcgggga cgtcatcgac caggtggtgg cgggaggccg
    12421 ccacctgacc gtcgtggccc gcctgcgcag gcccctgccg gagtcacctt ccctggtcct
    12481 ggtggtcccg cccttcgcgg agaggaatcc cggagcggcc cgggcctacg ccctcatgcg
    12541 gcggatcctg ggcggcgttc ccgccaagct cacggtgttg accctggcct ccgactcctc
    12601 cggggtccgc gattccctgc ggcgcatccg gggagcggct ccccacatcc aggagctgga
    12661 atcctggaag gaattctccg gtgcgatcgc caagcagccc gagggaaccg ccttcctctt
    12721 cctctgtccc cggccgggac gcctggcctg gcatccctac ctggaacagc tgccccggga
    12781 cgcggccgag gccttcccgg actcgaccct gatggtgacc taccttccgg acgaaggagg
    12841 cggcgcggag aactacccgg ccgcggccgc ggagtccgtc tcgggtccgg ccggggaggc
    12901 ggccggcccc gctgcggaag gcacgacgcc cgcgccggct ccggccgccg aggggatacc
    12961 cgagcccgct gctcctcccg actcctcgga cctcttctat cggaccctgg ccgggtccgg
    13021 ccgggtcatc cccgacttgc ccgcggcggc cgtggtggac gccgtccgga tcctcctgga
    13081 acgggggttc ccgggcgacc ggaggagcgt gaaccgcctg accgggatct acgcggagat
    13141 agcccagaaa cagcccatcg agctggcccc cggagtcctc ctcctgcacg cccacgtgga
    13201 caatatcgag gagccccgca tcctgatcgg agccaagccc gcgggactcc gtctgctggc
    13261 cctggaggag cccatccgga tcgtggtcct gctctgcgcg ccgagttccc agtccccgga
    13321 ggaccacctc cggacccttt ccctgatcgc ccaggccctg aagcgcccgg aacgggtggc
    13381 ccggatcctg gccgccaaga ccgaggagga tctccgggaa gactcctagg agcctgtcgg
    13441 acctgtagat ctttagcgcc tatcggcgct aaagattccg attatccgcc tcctagtccc
    13501 gcccctattc cgccatggac accacggcca gggcgccccg tccggcgttc agggcgatga
    13561 ccgaggagat ctccatgacg tactccgggg ggaagcccag ggccgcctcc agacgccgga
    13621 ccaggtcctg cgccccctcc gggtcgtggg cgtgcaccac cgcgtagttc cggagcttcc
    13681 cggaccggtg cttggccagg gtcatatcca ggatcctttt caggttggcc tccacactga
    13741 aggccttgcc gtacagtttc gaggagccgt ccctctccag ggacacgatg ggcttgaggt
    13801 tcaggatctt cgcggccagg ccctggaggg ggctgacccg gccgccccgg accatgtagc
    13861 gcagggtccg cacggagacc aggatgtccg ccttgtcgga ccaggccgga aggtccgcca
    13921 ggatgcggtc gtgggaccgg ccctccgcca cggcccgggc ggtccggagg accacgaggc
    13981 ccagggagcc ggacaggtgt cgggaatcca cgacgtcgat gcgcttcccg gtctccgccg
    14041 cgacctgttc cgccgcgcgc cggctggcct ccagggtgcc ggaaagcttt cccgcaagat
    14101 gcaccgcgat gatcgaatcg taacgttccg ccaggaaccg gtaggtccgg aggaagacgg
    14161 cccccgaggg ctgggaggac ttggggaagg acggggccct gtccgcgagc tcgtagaaac
    14221 gctccgggga gagggtcctg cggtccaggt attccacctc ccccacccgg atatggaggg
    14281 gcacgacgtg gacctggtat tcctccagga gcgccgcggg aaggtcgcag cccgaatccg
    14341 tgaccacggc gatggggtat ttacggacat gggcggtcag gaactgctcc ttcatgtcgt
    14401 ccaccttctg ctggaggacg tccccccggg cggacaggcg ttcgatcagg tccgccggac
    14461 ggtccgtgtg gatatggatc cggagcttcc tgcggttccc ggccacgatc agggaatccc
    14521 ccaggtcgga gacctccgac cgtatggatt ccgggtccag gtcccggccc accaccaggg
    14581 cttccgtgca gtaccggtgt ctgagttccg aaaggtcgtg gaggtccggg gcttcccctc
    14641 ccggatccag gtccgagatg tccagggctt cgtccgcatc ctctccgcct tccacgtagg
    14701 agtgggcccc cgccacaaag gcgacgaagc cggaagcccc cgcgtccacg accccggcgg
    14761 ccttgagctc cgccaggcgg ttgggggtgt cggccaggga gctgtgcagg gccggaaggg
    14821 tgccccccag gagttcccgg aaattccgtg cccggccgag ctcggcccgc agggcggccg
    14881 cccagtcccc gatcagggac aggatggtgc cgtcccgggg gttggagatg gcggaacggg
    14941 cccgggaata ggcgttttcc accgcggcca cgaacttctc catgctgatg gtggccgcgt
    15001 ccgtgagggc ctcgtagaat cccgagatga actgggcgaa gatgaccccc gagttccccc
    15061 gggccccggc caaagccgcg tcggctatgt tctccatggt cttgccggcg gaatccgtgg
    15121 gccgggaact ctccaccgcg gaggccaggg tggcggccag gttggtaccg gtatcgccgt
    15181 cgggcacggg aaagacgttg atccggtcca gctccttgcg gactcccagt acgcgccggg
    15241 cccccgcccg gaatacccgg tagatctgat gtccgtcgag ttcgtgcatc ggtcctccac
    15301 gttcggctga aaccgccggt gcctcatcgc gaatccaagg atactggttc gacacccccg
    15361 cggcaataaa aattgcagga atcgatcctg aaatgaatcc ggggggcctt cgccccccgg
    15421 attcattcgt tccgccgcgg cccccgagta tcgggctcct aggcgccgtg ccgctactcc
    15481 cggatcagga tgaactccac ccgccggttc ttccagcggt tctccgcgtc cttgaagtcc
    15541 accacgggct cgctggagcc caggccccgc accgacaggc ggcgggcgtc cacgccgaac
    15601 tggaccagga gccttcgcac cgcctccgca cggttcgtgg agagcgggat cagctcctcg
    15661 gtctcctcct tgttcacctg ggccgccgag tacccgtaga tctttccgat gctgttggcg
    15721 tggccctcga tccggatctt gtaatcccgg aaccggttga ggatctgggc gatccgcctc
    15781 aggaccacct cgttccggtc ggtcttttcc ttgggcaggc catcgaaatc cgcgccgttg
    15841 gagcggaaca cgatggaggg aaccttgatc ttgagccggt ccccgtcccg gatgaccagg
    15901 acgtccacgg tgacctttcc ctcgaccttg gccgagttgc cccaggcgtc catcacggcc
    15961 atcgcgtagg gatagtccga cgcgccctcg accagctcgc ccttgttgga gcggccgtcc
    16021 cagagtatgc gctccgtggg attccccgtc cccgaccagg aggtgaagag ccggaccttc
    16081 gcgggacccg ctccctcctc cacggaggac tcgaagatct cgaactgcca ggtctcgatc
    16141 gggctggcgt catcgatggc gatggccacg gtcagctcgt cgtccacccc gtcgttgtcc
    16201 gggctgaaga gctccgggct ggtccgcagg cggacccggg ggccctcggt gtccaggacg
    16261 aaggatgccg tccgggcctg gggggcgtcc cccttggcgt agaccaccgt gaacaccgcc
    16321 gtataggtgc cctggggcgc cgggtttccc cggtcgtcct tgccgtccca gacgagttcc
    16381 cggggcaggg aggcgatgtt cgcacccgag taggtcttgc gggccgtgcc ggcggcgtcc
    16441 gccagagcga ggttccagga ctccaccccg tcccgcaggt tcacgatggg gctgaacagg
    16501 atgtcgtcgt agcggccgtc cccgttcggg ctgaagcccg cccggttcgc ggtcacgaag
    16561 acctggaccg cccgggtatc cgccgcgatt ccgtcgatcc ggtactcggc cttgttgccc
    16621 gcggcgtcct cgctggacag cacgtaggaa taccggccgt ccgacgcggg attcccctcg
    16681 tcgtcccggc cgtcccagga gacggggaca agggcgcccc tccaggtcca ggtgcgcacc
    16741 ggccggccgt tggcggccag gatgcttccc cgccaatcgt ccccgggggc cgccgactgg
    16801 gtgatcacca cctcgtcctt gcggccgtcc ccgttggggc tgaacagcag gggcgcggcg
    16861 gatacctggg ccttgggcgc gacggtgtcc aggatgatga ctccggaggt ggccacggcg
    16921 gtgtccaggt tggcgaagcg caccgtcagt tccgcctcgt acgagccgtc cggggcctgg
    16981 gttcccccgt cgttccggcc gttccagacg agttcccggg gaaccgcgcc ggttcccttc
    17041 caggagcgga ccgcggcgcc gccggccgag gacttgacga ccacgtccca ggaggcgggg
    17101 gcgtcggagg atcggacttc cggaacgatg cggaccgagt ccttgacccc gtccccgttg
    17161 gggctgaaag cccgggcgtc ggaggatacg acggcggcct tcttctcggt atccacccga
    17221 aggttcacgg gcgccgagga accggagttc ccggctcgat ccacggaccg gagccggaag
    17281 gcgtaggcgc cgtccgggac gatcttgccg gattcgtctc ggccgtccca ttcgatacgg
    17341 gcgtccggtt ctcccgggaa ggaccaggtg cgcacggcct tgcccgcggc gtcggtgact
    17401 tccgcggtcc aggcgagctc ccggctgccc gaggcggaca gggccgccgc gttctgcccc
    17461 gccgcgccgg cggggttgaa ggtcggccgc tccacggaag cccgggccga ggggggcgtc
    17521 acgtccagga ggaagagggg ggaggtggcg gagggagcgt acccgttgac gtaccgcacc
    17581 tccaggaagg cccggtagct gccctcgggc aggggctttc ccgcggcgtc cttgccgtcg
    17641 aaggcccagc gctccggcag ggaggccgcg tcggatcccg tgcggctcca ccgctcggtc
    17701 cggccctcgt ccaggacggc caggcgccag gaggccaggc cggtccggat ggggaccccg
    17761 ggcaggaggg cgaccgtgtc ccggaccccg tccccgttgg gactgaaggc cgagacgtcg
    17821 atggccaccg tgatcggcgg ctgctgggtg ttcaccacga tgttgtccag gcgccgggag
    17881 acggtgttgc ccgcccggtc cgtggactcc accacgaagg agtacacccc gtcgggcacc
    17941 acggttcccg cgtcgttccg gccgtcccag gtcaggtcgg cgggggcgga ggccttgtat
    18001 tccacggtct tcaccgaggc gccggaggcg tccaggacgc gggccgtcca aagatcctcc
    18061 tgggagccgg tcttgcggat ggtcagggtg tccttgccgc cgtccccgtc ggggctgaag
    18121 atcaggtcca ccgcgtcccg gggcgcggcg atctcgaccc ggggaggcgt ggagtccacg
    18181 aagacctcgt ggaccttgct ggttcccttg ttcccgttgt cgtccaccgc ctccacgacg
    18241 atcgtgtacc ggccgtcggg gacgggcttc ccgtcgtcag cgaagccgtt ccacacgagc
    18301 ttgtccggca cggggatccc cttcttgacg taggccagcc ggtcccggaa gcccgccagg
    18361 cccgaggtct ccggccggct ttccttgttg ccgattaccc gcaccgcgga accctccgcg
    18421 ggaaccgcgg gcgcggcctc cggggaagcc ggcgcggccg atcccgcggg agcgggatcg
    18481 cctcggtaca cggtcatggt gtagccggac aggtagcggc gctccttgat ggacagggga
    18541 agctcgatct ggtcgttctt cccgtccgag ttgggggaaa ggtagtaggc tccccagggg
    18601 gtctccggat aggtcagctg gacgacgggg gcctcccggt ccacgacgcc cagggggatg
    18661 ttgatccccg cggaataggc gtagatcccg tcgtacaggg gtttgaccgc caagcccggg
    18721 cggatctcgc tgcggtccca gccctgcttg gagatgaagg attcgtcgga ctttcggttg
    18781 agctcgaact tcccgaagag cccgagggaa ggcaggaggg cggcggggct gttcaggatc
    18841 tcgtgtacgt tgagtcccca gcccaggcgc aggatcacct tttcccggaa ggagccttcc
    18901 agccccgcgt tgaccaggag gttctggaag ctcggggctg cgaggtcgag ggaggctccg
    18961 gcccggaatt tctccgtctg gatgaaatcc ccgcgccccc ccaaggtcag ggtataggga
    19021 cccgggaagg ccgtggcggc gccgcccgcg gcgccgagcc cggccgggac gtagggcttg
    19081 cccaggccgg agagcacggc gccccattgg aaattcttga agaacaggaa gtctccgacc
    19141 ttgcgcagga tgccgaggtc cgcggccagg ccccagtcgt ccccgatgac caggcccgcg
    19201 ccgatgccgg cgtacaggtt ggggtacagg tccttggcga tcgatccctc cacccggaag
    19261 gccgtgccca ggggcaggct ggtcagggag tccggtatgg acaggaactg ggcggaagcc
    19321 gtccagaccc cgtagggctt gggtatggtc agcccgaggt tcagggccga gccccagccg
    19381 gtctgggcgt ccatgcccac cacggccgcg tagttcgcgt ccaggaccac cctctgctgg
    19441 cccgccgagg ccgcgggatt gaggtaatcc gaggcgggtg actccgtgga ggccgcccag
    19501 gaacccccgg ccaggaggaa gggcgaggtc aggagctggg cgtattcgcc tccccggggc
    19561 ggatcgtagg ccggggcgga aaggaccgcg agggccgcga acaggatcgc cgggaacgcg
    19621 ggacggaagg ttttcatctt cggctccttt ttgaggtcgt gaggatccgg gcatcgggag
    19681 tgagcgatct cgagcacggg taaaaaataa cctttacctc gggactcggc aaatcgacgg
    19741 cgcggcggac tcgaaaacac acctaaagaa cgctggaacg tcgagttggt tacggtcccg
    19801 gggttcccct atgagtatac catcatccgg cgtccgtcac ccgcgtcact ggtacatgat
    19861 caacacggga gccggagcga gccccaggac ctcctggggg gtcatgaggg gctcgtcgaa
    19921 gccggccccg gggtacccgc tcttgaagaa gagcttgaat cccttgaggg gcaggttcgc
    19981 ggcccgggaa ttcagggcgt aggaataccg tttgagcgcc ggggggccga agccgtccat
    20041 cacgtgcacg ggaagcaccc ggtcgaagtc cgcccggacc cgggaccggt cctggatcat
    20101 cttttccgtg aactggtgga agaccaggat tcgcaccccg ggcagaccgt gctccgtcag
    20161 gtaggcctgc atgagctcct gg
//
LOCUS       DAOTEI010000047        19960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1779_length_19960_cov_5.205828, whole genome shotgun sequence.
ACCESSION   DAOTEI010000047 DAOTEI010000000
VERSION     DAOTEI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 19960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19960
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1779_length_19960_cov_5.205828"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="P5313_06495"
     CDS             complement(<1..632)
                     /locus_tag="P5313_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY80044.1"
                     /translation="MDSVIGRQRISPRRKPAPLIAPVPRPRPRTARAARWPAAFAPGR
                     LARLFFRGKAEAKSVPAPSSAVPAARLRISARSAGIAGAGILTVLAAFGAAGGISAGY
                     RPPARMTLHEEVSSHEILSRFVSPELGEPGDAGTGIDMPSLPLTLSVSTYVVRPGDAL
                     SSIAARHGVSLDALISMNGISDARRIRAGTRLRIPNLSGIVHVVARGESVG"
     gene            691..1194
                     /locus_tag="P5313_06500"
     CDS             691..1194
                     /locus_tag="P5313_06500"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRY80045.1"
                     /translation="MSNGITESGNPVPADGRIRVPGSRTRLPSGGGALLTATALALAI
                     KVFALDLAVVEGRSMEPFVSRGSLVLVLKCAYGLRAPGREGSYWFRWGSPAPGDTVLA
                     YPPSTGTRVVKRVASEASPYGSYPEGPRGRPIYLLGDNPPASRDSREYGPVGMDDIAG
                     KVIRLRP"
     gene            1238..3109
                     /locus_tag="P5313_06505"
     CDS             1238..3109
                     /locus_tag="P5313_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRY80046.1"
                     /translation="MERRHLRYWTAISLLAAFAAVVTARYAKLALEGPSPASVRPPEV
                     VRGSILDRNGRPLAVDTNLYNIAIWRPELDKDLWIEDAGELERVLGLEAGSVRKVVEA
                     SRTDFLYAVKRIPASRAQEVQELKRRRGLSGFLVEKVHGRLYPERTLASHVVGFVGDE
                     HYGLAGVEARYQNELSPRVDPGEPGNIVYGNQVILTLDADAQFHLERIARKALQDNGA
                     ESVMLLAQRASTGEILAYVSLPDFDPNDFLAYPETAWSDRPALYAFEPGSVFKVFSMA
                     GIMELGAIDERSTFVCDGAYERVVSPEETIRIRCLGNHGRVSVKEILEYSCNDGAGQA
                     SDTVAALDFYDILREFGFGMRTGLPLPGESPGSLRDPKTWSLRSKPTIAMGQELLVTA
                     AQMTAAAGAIANGGILMKPRLVSRVLAPDGSTVSEEPAQPVRRVLSPENARKILAAME
                     AAAGEAGTGRRAAISGVRLAVKTGTAQMIDTGTGRYSDKDFIASCVGIFPADEPEIVL
                     YLAIVKPRGESTFGGRIAAPVIREAAESLITLYGLARGSTPTLSHTGRIVLTKPVPVE
                     IGETMPDLTGTPKRLLLPLLAREDLEVSVQGDGYVVSQEPPPGTPVSPGTKIRLVLE"
     gene            3106..4929
                     /locus_tag="P5313_06510"
     CDS             3106..4929
                     /locus_tag="P5313_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRY80047.1"
                     /translation="MTGPGSESPGSPPEGFREGNLRALGRRFPELETLLRGAAPASLE
                     SVRTPSGFPTARAGGRYLHSRYDPEGEARKSAAALASSGADALVVLGPGLFYAAEAAL
                     RELPEARVVAACADPAFLAACLDLRDLGGLLSDDRLSILAGGAPEGLLWIFERLGTRK
                     VRVLESKAEAELYPEWYGRAKAILARWEAKETINTATLRRFGRLWVRNLARNLDEIPR
                     HPGVAGLEGRFEGIPAVVAAAGPGLDEVLPLLSVLRDRAVLVCVDTALRSVLRAGIEP
                     DFLVVVDPQYWNARHLDRCRALRSVLVTEAAVWPSILRMPFASVRLCASLYPLGTFAE
                     GRAGGSKGRLGAGGSVATTAWDFARVLGCRPIYMAGLDLGFPGGRTHARASRFEQKAL
                     AEGRRLRPPSTYLYEAYAGARPRDARANDGGVVRTDERLALYSWWFESRMLRHPECTT
                     RNLSARGLAIPGMPYEPAEELRSLPPARDRIDAALEILRREEREGAAAEGPPSDARGR
                     VLGLLSDMEARAGAALDLASRARALERAGRDPSEVLALLDEADRDLLGNEAKEIAGFL
                     LPDLETLLGRPARTLEEALERSEAVYREIRDSAAYHRSILT"
     gene            complement(4921..5919)
                     /locus_tag="P5313_06515"
     CDS             complement(4921..5919)
                     /locus_tag="P5313_06515"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRY80048.1"
                     /translation="MRPEALALHRDSVVVDGHCDSVLELLGFGFDGQDAQPRDLAVRG
                     SRGHADIPRLLDGGVTAQVFALFTDDKYVPDAAAHTRRMLEAMEALFERTDRVRRALS
                     ARDVEEAKAAGRLAAFLGIEGGEAIGESLAELRGFYARGVRLMGLTWNRRNALGRGAA
                     AEGTGGLTDFGRKVVAEMERLGMIVDASHLSDEALTDLLAVARRPVVASHSNSRAVRD
                     HRRNLTDAQAEGIAATGGLVAVTFAGIFVDEAPEKVCLARLLDHVDRLVSVVGTDHVG
                     LGSDFDGFLDKNGTVLPDCTGLPLLTEALLDRGYAPEEVRAVLGGNWMRVIRDVLG"
     gene            complement(5916..7184)
                     /gene="lysA"
                     /locus_tag="P5313_06520"
     CDS             complement(5916..7184)
                     /gene="lysA"
                     /locus_tag="P5313_06520"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRY80049.1"
                     /translation="MAHLLFDGCDAVDLAREFGTPLYVLSEDRIRVRIRELRETFLDR
                     WPGTSAAYASKALQTLDLCRIVASEDLDLDVVSGGELYAALKAGFPPGRIHFHGNSKT
                     PDEIRMALDGGVGRMIVDNRTELDLLDREARARGIRVPILFRVTPGVDSHTHQYMSTG
                     SLDSKFGIPLDPAVRDEYVEAALRMEGIELRGFHFHVGSQIFSNESHRKALRIVADLA
                     AEVRDRYGFETRELILGGGFAARYTAQDEPEPLAYYTDALMTDLRDLCAGSGLPMPRV
                     GIEPGRWIVAEAGITLYTVGSVKEIPGVRTYVGVDGGMPDNIRPALYGAEYEATVADR
                     HDEPRTRTVTVAGKCCESGDILIRNVALQDPRPGDVLAVFTTGAYTHSMASNYNRLPR
                     PAMVLVRDGKARLSVRRETYEDLVARELLS"
     gene            complement(7194..7994)
                     /locus_tag="P5313_06525"
     CDS             complement(7194..7994)
                     /locus_tag="P5313_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HRY80050.1"
                     /translation="MNKLYISADIEGVSGIVDWRETDNGDFQSAYFRAQMTREVKAAC
                     EAAAEAGVTDILVKDAHGTGRSIDPSALPRNACILRSWTKDPLVMMGGFDAGFEGVFF
                     IGYHSMAGSGGNPLSHTMDLNVQELRINGQPVSEFVINAYTAASMGVPVLLLTGDREL
                     CETAREYNPNLVTVPVSEGVGNGSVSIHPDLAAERIKAAAASVLAEDPSKFRFPLPDS
                     FDVSIVYKQHYRAYRASYFPGAKLASPTEVGFRSRKWEDVLTFFLFVL"
     gene            complement(7991..9115)
                     /gene="alr"
                     /locus_tag="P5313_06530"
     CDS             complement(7991..9115)
                     /gene="alr"
                     /locus_tag="P5313_06530"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRY80051.1"
                     /translation="MIDRPAWAEVNLSAVTRNVRYLKSLMSPGARLCAVVKADGYGHG
                     AAGVSRAALAGGADRLAVAFAEEGIELRRQGITAPILVLGYTGPEGARRAVGWGLDVT
                     VLSADNARLVSEAAVSLGRKARVHLKVDTGMSRLGIRPEEAPGAARAIAALPGLELEG
                     VFSHFAASDAADPEFAREQLALYRSVLGRIEEAGLRIPLRHMANSGGLLFLPESRLDM
                     ARPGISVYGLRASSERESLAGLEPAMALRARVVQTRTVPAGTTVSYGRTFRASRTTRL
                     AVLPLGYADGVFRSLSNRGSVGFPSGRAPIAGRICMDQCMADVTDLPEVREGDVAVFF
                     GPGGPSAGEVADLLGTIDYEITCAVNKRVPRIYTEEETES"
     gene            9576..10634
                     /gene="selD"
                     /locus_tag="P5313_06535"
     CDS             9576..10634
                     /gene="selD"
                     /locus_tag="P5313_06535"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:9624..9626,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRY80052.1"
                     /translation="MDRQTIRLTQLCSAAGUAAKMGPEALAQVLRPLTALVPPERKAD
                     LIVGLAEGDDAAVLKLDAEKALVFTVDFFTPVVDDPYDFGAVAAANALSDVYATGGEP
                     LMALNVAAFPADLDPAVIAAVVRGGAEKAAEAGCATAGGHTIQDAEPKFGLAVIGLAH
                     PDRLFLKTGVRPGDSLVLTKPLGTGVITTWAKNGAVDPAVLAGAVESMKRLNRNAARV
                     FARFGVKGCTDVTGFSLAGHALELAEKSSVRLEIDAGALPLLPGARAAVEGKFVPGGA
                     KRNRSYYEDRVTFADGVDSVVRTLCFAPETSGGLLAAVPGDAEGCVRELRDLGVEAAV
                     IGRALPAVDGAVPGIGFR"
     gene            complement(10868..11887)
                     /locus_tag="P5313_06540"
     CDS             complement(10868..11887)
                     /locus_tag="P5313_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRY80053.1"
                     /translation="MNAKPRILIADGDPATELLCKSLGPEYEFIYARNDREFWEYSFG
                     DPRPDVVLLDALFWGSDPLTLLARFREKEETIQRVPIIFSAPFFDRNAVDAAIRGGAS
                     DFLLKPHAPGILQLRIRTQLELSRSRAELESLVEERTKELRDSHLEVVRRLVRASEFR
                     DNETGAHILRMSRYSQIIASAAGADAATAELVLNAAPMHDIGKIGVPDAVLRKPGPLN
                     EEEWGLMRMHCRIGANIIGKGDSEIARHAAEAALTHHERYDGSGYPEGLSGERIPWIG
                     RVIAVADVFDALTTERPYKKAWPPELAVDLIRSESGTHFDPKLVSAFLQSRDEIIEVR
                     ALYPD"
     gene            complement(11921..12589)
                     /locus_tag="P5313_06545"
     CDS             complement(11921..12589)
                     /locus_tag="P5313_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY80054.1"
                     /translation="MNTRSTILDTALELFRSNGFESTPMDSIATAADVAKGTLYYHFD
                     SKEGIIDAIVDREAAAVREQLIRVELDSDLGFEDRYQAAVAIMTNLIGATYAKLQKIK
                     YIDIRDKTMLAMVEFCAPPFARILEQGNAAGRCRVEHALEFAELTLTFAQFLGSPGAG
                     EPSIPRRIKALVRFSSVLLGMDAQTVERVYAPLRKQFEDSPAPAGEGDPIVDDLSARG
                     RRPV"
     gene            12717..14177
                     /locus_tag="P5313_06550"
     CDS             12717..14177
                     /locus_tag="P5313_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY80055.1"
                     /translation="MDAIPRTLLLVEDESIIALAEARTLSSAGYSVIHVGSGEEAISA
                     MNGERGSSIDLILMDIDLGEGIDGTEAAYEILKRHDIPIIFLSSHSDPKTIEKTEKIT
                     NYGYVVKNLGHTMMFAAIRMAFKLHTAQAKIREREEWIRDLIDKIEDGICILDEKDRF
                     LYANSALSGVFGVEHGRLVGLPLSTMVGESALETIRANLQLSDVRPVGETSLTVEGAD
                     GISRALQLKIIQDYDAKHKYRGSLVAVRLQAAESGTGAPAGDRGTVRNRKARDVYVHE
                     LLHRIKNSLNIIVSLLSLEAQRVSDEPARRMLVLTETRIRSISLLYDFLSHSTGEDRI
                     DSVEYIQELTRLLKETYSSPDSRIEFITSADPFPLSSKVCLSIGLMVNELISNALKYG
                     FPRGREGTIRIELRKEADSLILRVRDDGIGLPEDYFPNENLGFGLHLVKTLASQLNGT
                     MNLVNDRGVCVTITARVPQEATDAGGPGEQKVGASA"
     gene            complement(14299..14778)
                     /locus_tag="P5313_06555"
     CDS             complement(14299..14778)
                     /locus_tag="P5313_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY80056.1"
                     /translation="MECDSGTSYLTFSIHEVRFAVNVKNVREVLEYSPLTRLPGKEDS
                     SQGIINVRGTCIPVVDLGLVLGMAVPPVGESAAILILEVGKGDGAFLAGAVVDAVYGV
                     ANFSTPMIDPPRFGPVRPSEYLVGIGRGRDGFVLVLDVDRVFSADRVGNSEDLVEAV"
     gene            complement(14802..16802)
                     /locus_tag="P5313_06560"
     CDS             complement(14802..16802)
                     /locus_tag="P5313_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY80057.1"
                     /translation="MNKGVSFGVRIALIIVAFLAVMIGGITILISIRMKDSMNDLALS
                     YNRQIVDARVSQLSEMMDKLFWQQRMIAEQDVLKRGDREAVESAILGLRGKLSPEVVT
                     VFYVWPDGESVTADGARANVQDRDYFDAILRKGKDRFIGRPVISRSINLPIVVPVVAV
                     QDPEGNTRGILGFQFKLETLSTITSGIKIGKSGYGWVVDDTGLVIAHPNPKAVMALSL
                     SDADKQGYKALDDFGKRALSEDSGNGRYFLPDGTEVTGFFRRIPNTPGWSFGLSIPTS
                     EIQETSTSLARILYILTLCSLALAFLLALLIARIIVNLVKIIVSSMEHLSDGELYLGG
                     PERLLLDRAASRTDELGMLAKEMEQLREKLASVFQEIQSAAVQVSSGSGQLSEMAQGL
                     SSGATEQAASVEELSASVEELAASAGQNTESTRQIEALAARVRQNAEDAERSVTTSSE
                     SMRVIASKIGVVEEIARQTNLLALNAAIEAARAGEAGKGFAVVALEVRKLAERSATAA
                     SEINGLSSDGVAVADEARARLEALVPDIKKTEDLIREIAAASEEQSSGVEQMSKGAAQ
                     IDLVIQQNAANSEELAATADELAGQANSLSEAIGFFKFSEAGMVYSVEGAAEARPAQP
                     ARIATVPAAPRPAATGRPSSTAIAVRKTMAASGVTGEEFEEF"
     gene            17090..17584
                     /locus_tag="P5313_06565"
     CDS             17090..17584
                     /locus_tag="P5313_06565"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRY80058.1"
                     /translation="MQNDAIRILITGGTFDKHYDEIHGELTFKDTNLPEIIKQIRTTI
                     PVELEINQLIDSLQMQEENRERVLGACRRAPESRIIVTHGTDTMAVTARVLGEAGLDK
                     TIVLTGAMVPYKVSGSDALFNLGTAFAAVQLKPPGVWIAMNGRVFPWDRVRKNKAGGV
                     FEEI"
     gene            17587..18693
                     /locus_tag="P5313_06570"
     CDS             17587..18693
                     /locus_tag="P5313_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRY80059.1"
                     /translation="MPKSPSPGRRAAAGRRTSGAARAAGPGRGRRSPSPRPGKPHPVL
                     YSAAAVLVLAALAGAAWITGAFPGRESPGPGAAAPEALRPSDSAAPSLETRSYGAEAA
                     PERSPVPEAPPERPAGGTVVRTGAGRSPGGVPERPKPPKGKLVLVIDDAGHNLRDLEP
                     FLAFPGPLSIAVLPRLPFSEEAARRAKAAGKEVLLHQPMEALGGEDPGPGAVRLGMGD
                     LEIAALIAENLATVPGAVGMNNHMGSAVTRDPRMMEAVLEAARASGLYYLDSLTVSDS
                     VLHSVASRLNLRTWERDVFLDNYPERAAMLKQLDDGIKRAEKKGLSVMIGHVWSAELA
                     QTLIDLYPGLIEQGFSLSTISRIMLEDSDEDSGD"
     gene            18674..19705
                     /gene="tsaD"
                     /locus_tag="P5313_06575"
     CDS             18674..19705
                     /gene="tsaD"
                     /locus_tag="P5313_06575"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRY80060.1"
                     /translation="MRILGIETSCDESSAAVVEDGRRILSNVVATQIPFHAEYQGVVP
                     EIASRKHAEWISRVVAEALTEAGVSAADIDGVAVTAKPGLVGSLLVGVSFAKAWAWAL
                     DRPFVGVNHILAHLYAPLLSEDVDYPFLGLLVSGGHTLICRVDGFDDISILGTTIDDA
                     VGEAFDKTAKFYGFGYPGGLAIDRLARKGDPAACRFPSPNLYKGEHRYDVSYSGLKTA
                     AIRQLDQFWDPAYPRTPENIAAAFEKAAVDLLLSRLERAAQDTGLSTVVAGGGVAANT
                     YLRARLAGRTDLRALFPPLPLCGDNGAMVAGIGFKYLERGDRDGLDLNASPRVPIFRR
                     LDKPGRARD"
     gene            complement(19744..>19960)
                     /locus_tag="P5313_06580"
     CDS             complement(19744..>19960)
                     /locus_tag="P5313_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80061.1"
                     /translation="EVKLVPGNFRAYDFGFQGPATSDAATLRFLVIAYEADQEAAWLA
                     DAVFRRSGLKTRLTAVRKVSRGKPLVD"
BASE COUNT         3231 a         6825 c         6692 g         3212 t
ORIGIN      
        1 cccacggatt cgccccgggc caccacgtgc acgatcccgg agaggttggg aatccggagc
       61 cgggttccgg cccggatccg gcgggcgtcg gagatcccgt tcatgctgat gagggcgtcc
      121 aggctgactc cgtggcgggc ggctatggag gacagggcat cgccgggccg gaccacgtag
      181 gtcgaaacgg acagggtcag aggcagggaa ggcatgtcga tcccggtacc cgcatccccc
      241 ggctccccga gctcggggga gacgaagcgg gaaaggattt cgtgggagga aacctcttca
      301 tgcagggtca tccgggccgg agggcggtat cccgccgaaa tcccgcccgc ggccccgaag
      361 gccgccagga ccgtcaggat gcccgccccg gcgatcccgg cggaccgcgc cgaaatccga
      421 aggcgcgccg cggggaccgc ggaagagggc gccggtacgg acttcgcctc ggccttcccg
      481 cggaagaaga gacgcgccag gcggcccggc gcgaaggcgg cgggccatcg ggccgcccgg
      541 gcggtccggg gccggggccg cgggaccggg gcgatgaggg gggcgggctt ccgccggggg
      601 ctgatccgct gacggccgat gacgctgtcc atatccatgg cgttccattt atcgacaaaa
      661 gccggacggg ggattatagt aggtcgcgcc gtgtcgaatg gaatcaccga atccgggaac
      721 ccggtcccgg cggacggaag gatccgcgtc cccggatccc ggacccgcct gccctccggg
      781 ggaggcgccc tcctgaccgc cacggccctg gctctggcga tcaaggtctt cgccctggac
      841 ctggcggtcg tggaaggcag gtccatggag cccttcgtat cccggggctc cctggtcctg
      901 gtgctgaaat gcgcctacgg actccgggct cccggccggg agggaagcta ctggttccgg
      961 tggggctctc cggctcccgg ggacacggtc ctggcctatc ccccctccac cggaacccgg
     1021 gtcgtcaagc gggtggcctc ggaggcgtct ccctacggat cgtatccgga gggaccccgg
     1081 gggcggccga tctatctcct gggggacaac cccccggcat cccgggattc ccgggaatac
     1141 ggtccggtgg gcatggacga catagccggc aaggtgatac ggctccggcc ctgaaacccg
     1201 atcctgaacg aagaaaggtc cgatcgacgg aaatccgatg gaacgaaggc acctgaggta
     1261 ctggacagcc atttccctcc tcgccgcctt cgccgcggtc gtgacggctc ggtacgcgaa
     1321 gctcgccctg gagggcccct cgccggcctc ggtcaggccc ccggaggtcg tgcggggctc
     1381 catcctggac cggaacggcc gccccctggc ggtggacacg aacctgtaca acatcgcgat
     1441 ctggcgcccg gagctggaca aggatctatg gatcgaggac gccggggaac tggaacgcgt
     1501 gctggggctg gaggccgggt ccgtccggaa ggtcgtcgag gcaagccgga cggacttcct
     1561 gtacgccgtc aagcgcatcc ccgcctcccg ggcccaggag gtccaggagc tgaagcgccg
     1621 acggggtctc tcgggcttcc tggtggagaa ggtgcacggc cggctctatc ccgagcggac
     1681 cctggccagc catgtcgtgg gcttcgtggg ggacgagcac tacggcctgg cgggcgtgga
     1741 agcccggtac cagaacgagc tgtccccccg cgtcgacccg ggggagcccg ggaacatcgt
     1801 ctacggcaac caggtgatcc tgaccctgga cgcggacgcc cagttccatc tggaacggat
     1861 agcccggaag gccttgcagg acaacggcgc cgagtccgtc atgctcctgg cccagagggc
     1921 ctccacaggg gagatcctgg cctacgtctc cctgccggac ttcgatccca acgacttcct
     1981 ggcctatccc gagaccgcct ggtccgaccg gcccgccctc tacgccttcg agcccggctc
     2041 ggtcttcaag gttttcagca tggcggggat catggagctc ggagccatcg acgagcgctc
     2101 gaccttcgtg tgcgacgggg cctacgagcg ggtcgtgtcc ccggaggaga cgatccggat
     2161 ccggtgcctg gggaaccacg gccgtgtttc ggtcaaggag atcctggagt actcctgcaa
     2221 cgacggggcg ggccaggctt ccgacacggt ggccgccctg gatttctacg acatcctccg
     2281 ggagttcggg ttcgggatgc gcacgggcct gcccctgccc ggggagagcc cgggctccct
     2341 gcgggatccg aagacctggt ccctgaggtc caagcccacc atcgccatgg ggcaggaact
     2401 cctggtcacc gcggcccaga tgaccgccgc ggccggggcc atcgccaacg gaggcatcct
     2461 gatgaagccc cggctggtca gccgggtcct ggccccggac gggagcacgg tctccgagga
     2521 gcccgcccag cccgtgcgcc gggtcctgtc cccggagaac gcccgcaaga tcctggccgc
     2581 catggaagcc gcggccggcg aggcgggcac cgggcgcagg gcggccatct ccggggtgcg
     2641 cctggcggtc aagaccggaa ccgcccagat gatcgacacc gggaccggac ggtactcgga
     2701 caaggacttc atcgccagct gcgtggggat cttccccgcc gacgagccgg agatcgtcct
     2761 gtacctggcc atcgtcaagc cccgggggga gtctaccttc ggcggccgga tcgcggcccc
     2821 ggtgatccgg gaggctgcgg agagcctcat caccctgtac ggcctggccc ggggctccac
     2881 ccccaccctg agccacacgg ggcggatcgt tcttaccaag ccggtcccgg tggagatcgg
     2941 cgagactatg ccggacctta cgggcacccc caaacggctc ctcctgcccc tcctggcccg
     3001 ggaggacctg gaggtgagcg tccaggggga cggctacgtg gtctcccagg aaccgccccc
     3061 gggaaccccc gtctccccgg gcacgaagat ccggctggtg ctggaatgac gggtcccgga
     3121 tcggaatccc ccggttcccc gcccgagggg ttccgggagg ggaacctccg ggccctgggg
     3181 cggaggttcc cggagctcga gaccctgctc cggggcgcgg cgccggcctc cctcgaaagc
     3241 gtacgtaccc cctcgggttt cccgaccgcc cgggcgggag gccgctatct tcactcccgg
     3301 tacgaccccg agggggaggc ccggaagtcc gccgccgccc tggcctcctc cggtgcggat
     3361 gccctggtgg tcctgggccc gggccttttc tacgcggccg aggccgccct ccgggagctt
     3421 cccgaagccc gggtcgtggc cgcctgcgcg gacccggcct tcctggccgc ctgcctggac
     3481 cttcgcgacc tcggcggcct tctgtccgac gaccggcttt ccatcctggc cgggggggcc
     3541 cccgagggcc tcctgtggat cttcgaacgc ctggggacgc ggaaagtgcg cgtgctggag
     3601 agcaaggccg aggcggagct gtatccggag tggtacggcc gggccaaggc gatcctggcc
     3661 cgctgggagg ccaaggagac gatcaacacc gccacccttc ggcgcttcgg ccggctctgg
     3721 gtgcggaacc tggcccggaa cctggacgag attccccggc accccggagt cgccgggctg
     3781 gaaggacggt tcgagggcat ccccgcggtg gtcgccgccg cgggtcccgg cctggacgag
     3841 gtcctccccc tcctgtcggt gctccgggac cgggccgtcc tggtctgcgt ggacacggcc
     3901 ctccggagcg tcctgcgggc ggggatcgag ccggacttcc tggtggtggt ggatccccag
     3961 tactggaacg cccgccacct ggatcgctgc cgggccttgc gctccgtgct cgtgaccgag
     4021 gccgccgtct ggccctccat cctgcggatg cccttcgcct cggtgcgcct ctgcgcctcc
     4081 ctctaccccc tggggacctt cgcggagggc cgggccgggg ggagcaaggg acggctgggg
     4141 gccggaggct ccgtggctac caccgcctgg gacttcgccc gggtcctggg ctgcaggccg
     4201 atctacatgg cgggcctgga cctgggcttc cccgggggac ggacccacgc ccgggccagc
     4261 cgcttcgagc agaaggccct ggccgagggc cggcggctcc gccctccctc cacgtacctc
     4321 tacgaggcct acgcgggggc ccggccccgg gacgcccggg ccaacgacgg gggcgtcgtc
     4381 cgcaccgacg aacggctggc cctctactcc tggtggttcg agagccggat gctccggcac
     4441 cccgagtgca ccacccggaa cctgtcggcc cggggtctgg ccatcccggg catgccctac
     4501 gagccggcgg aggagctccg gtccctgccc ccggcccggg accggatcga cgcggccctg
     4561 gaaatcctga ggcgggagga acgggagggc gcggccgcgg aaggcccgcc ctcggatgcc
     4621 cgcggccggg tcctgggact tctatcggac atggaagccc gggcgggagc cgccctggac
     4681 cttgcgtccc gggcgcgggc cctggagcgg gcgggccggg atccctcgga ggtcctggcc
     4741 ctcctggacg aggccgaccg ggacctgttg gggaacgagg ccaaggagat cgcgggcttc
     4801 ctgttgcccg acctggagac gttattggga cggcccgccc gcaccctgga ggaggccctg
     4861 gagcgttccg aggcggtgta ccgggagatc cgggactccg cggcctacca ccggagcatc
     4921 ctaacctaga acgtcccgga tcacccgcat ccagtttccg cccaggacgg cgcggacttc
     4981 ctccggcgcg tagccccggt ccaggagggc ctcggtcagc aggggcagac ccgtgcagtc
     5041 cggaaggacg gtcccgttct tgtccaggaa tccgtcgaag tccgagccca ggcccacgtg
     5101 gtccgtcccg acaacggata ccagccggtc cacgtggtcc aggaggcggg caaggcagac
     5161 cttctccggc gcctcgtcca cgaagattcc cgcgaacgtg accgccacca ggccccccgt
     5221 ggcggcgatg ccctcggcct gggcgtccgt caggttgcgc cggtggtccc ggaccgcccg
     5281 ggaattggag tgggatgcca cgacggggcg ccgggcgacc gccagcagat cggtcaaggc
     5341 ctcgtcggag aggtgggagg cgtccacgat catgccgagc cgttccatct cggccacgac
     5401 cttccgcccg aagtccgtga gccccccggt tccctccgcg gcggcccccc ggcccagggc
     5461 gttgcggcgg ttccaggtga ggcccatcag gcgcaccccc cgggcgtaga agccccgaag
     5521 ctctgcaagg ctctccccga tcgcctcgcc cccctcgatg cccaggaagg ctgccaggcg
     5581 gccggcggcc ttggcctcct cgacgtcccg ggcggacagg gcccggcgga cccggtccgt
     5641 cctttcgaac agggcttcca tcgcctcaag catgcgccgg gtgtgcgccg ccgcgtccgg
     5701 gacgtacttg tcgtccgtga agagggcgaa gacctgggcg gtcaccccgc catccaggag
     5761 gcgggggatg tccgcgtggc cccggcttcc ccggactgcc aggtcccggg gctgggcgtc
     5821 ctgcccgtcg aagccgaagc ccaggagctc caggaccgag tcgcagtgcc cgtccaccac
     5881 gacggaatcc cggtggaggg cgagggcttc gggcctcacg acaggagctc ccgggccacc
     5941 aggtcctcgt aggtctcccg gcgaaccgag agccgggcct tcccgtcccg gaccaggacc
     6001 atcgcgggcc ggggcaggcg attgtagttg ctcgccatgg agtgggtgta ggctcccgtc
     6061 gtgaagaccg ccaggacgtc cccgggccgg gggtcctgga gggcgacgtt ccggatcagg
     6121 atgtccccgg actcgcagca cttgcccgcc accgtgaccg tgcgggtccg gggctcgtcg
     6181 tgccggtccg cgacggtcgc ctcgtactcg gccccgtaca gggccggtcg gatgttgtcc
     6241 ggcatcccac cgtccacccc gacgtaggtc cgcaccccgg ggatctcctt caccgagccg
     6301 acggtgtaca gggtgatccc cgcctctgcc acgatccagc ggcccggctc gatcccgacc
     6361 cggggcatcg gcagacccga tcctgcgcag agatcccgga ggtctgtcat gagggcgtcc
     6421 gtgtagtagg ccaggggctc gggctcgtcc tgggccgtgt accgggccgc gaagcccccg
     6481 cccaggatga gctcccgggt ctcgaagccg tagcggtccc ggacctccgc agccaggtcc
     6541 gcgacgatcc gcagggcctt ccggtgggac tcgttggaga agatctggga ccccacgtgg
     6601 aagtggaagc cccggagctc gatcccctcc atccggaggg cggcctcgac gtactcgtcc
     6661 cgcacggcgg ggtccagggg gatcccgaac ttcgagtcca ggctcccggt ggacatgtac
     6721 tggtgggtat gactgtccac cccgggggtc acccggaaca gtatgggcac ccggattccc
     6781 cgggcccggg cttcccggtc caggagatcc aactcggtcc ggttgtccac gatcatccgg
     6841 ccgacccctc cgtccagggc catccggatc tcgtcggggg tcttggagtt gccgtggaag
     6901 tggatccgcc cgggggggaa gcccgccttg agggccgcgt agagctcccc gccggacacg
     6961 acgtccaggt cgaggtcctc ggaggcgacg atgcggcaga ggtccagggt ctggagggcc
     7021 ttggaggcgt aggccgcgga ggtgcccggc cagcgatcca aaaaggtttc ccggagctcc
     7081 cggatccgga cgcggatccg gtcctcggaa aggacgtaca ggggagtccc gaactcccgg
     7141 gccaggtcca ccgcgtcgca gccgtcgaag agcaggtgcg ccatcgccgg cccctacagg
     7201 acaaagagga agaaggtcag cacgtcctcc cacttccggc tccggaagcc cacctccgtg
     7261 ggggacgcca gcttggcgcc ggggaagtag gaggcccggt aggcccggta gtgctgcttg
     7321 tagacgatgg acacgtcgaa ggagtccggc agggggaagc ggaatttcga aggatcctcc
     7381 gccaggaccg aggcggccgc ggccttgatc cgctcggccg ccaggtccgg gtgtatcgag
     7441 acggagccgt tccctacgcc ctcggagacc gggacggtga cgaggttcgg gttgtattcc
     7501 cgggcggtct cgcagagctc ccggtccccg gtcaggagca acacggggac ccccatggag
     7561 gcggccgtgt acgcgttgat gacgaactcc gagaccggct ggccgttgat ccggagctcc
     7621 tggacgttca ggtccatggt gtgggacagg gggttccccc cgctgccggc catggagtgg
     7681 tagccgatga agaagacccc ctcgaagccg gcgtcgaagc ctcccatcat caccaggggg
     7741 tccttggtcc aggaccggag gatgcaggcg ttccggggaa gggccgaggg atcgatggac
     7801 ctccccgtgc cgtgggcgtc cttgaccagg atatccgtca cccccgcctc cgcggcggcc
     7861 tcgcaggcgg ccttgacctc ccgggtcatc tgggcccgga agtaggcgga ctggaagtcc
     7921 ccgttgtccg tctccctcca gtcgacgatg ccgctgacgc cctcgatgtc cgcgctgatg
     7981 tagagcttgt tcacgattcc gtctcctctt ccgtgtagat gcgcggaacc cgcttgttca
     8041 ccgcgcaggt gatctcgtag tcgatggtcc ccaggaggtc cgcgacctcc cccgcggagg
     8101 gtcccccggg gccgaagaag acggcgacgt ccccctcccg gacctccgga aggtccgtca
     8161 cgtccgccat gcactggtcc atgcagatcc ggcccgcgat gggcgcccgg ccggagggaa
     8221 agccgacgga accccggttg gagagggacc ggaagacccc gtccgcgtag cccaggggca
     8281 ggaccgccag gcgggtggtc cgggaggccc ggaaggtccg gccgtagctc acggtggttc
     8341 ccgccgggac ggtccgggtc tggaccaccc gggcccgcag ggccatcgcg ggctccaggc
     8401 ccgccaggct ctcccgctcc gaggaggcgc gcagtccgta gaccgagatg cccggccggg
     8461 ccatgtccag ccgggattcc ggcaggaaga ggagcccccc ggagttggcc atatggcgca
     8521 gggggatccg gagcccggct tcctcgatcc gccccaggac tgagcggtac agggcgagct
     8581 gttcccgggc gaactccggg tccgcggcgt cggaggccgc gaagtgggag aagaccccct
     8641 ccagctcgag ccccggaagg gccgcgatcg cccgggccgc ccccggcgcc tcctccggac
     8701 ggatgcccag gcgggacatc cccgtgtcga ccttgaggtg gacccgggcc ttccgcccca
     8761 gggagacggc ggcctcggag accagccggg cgttgtccgc ggagaggacg gtgacgtcca
     8821 ggccccagcc cacggctcgg cgggccccct cgggccccgt atagcccagg accaggatgg
     8881 gggccgtgat cccctgccgg cggagctcga tcccctcctc cgcgaaggcc acggccaggc
     8941 ggtccgcccc cccggccagg gcggcccggg agaccccggc ggccccgtgg ccgtagccgt
     9001 ccgccttgac caccgcgcag agccgggcgc ccggggacat gagggacttg aggtagcgga
     9061 cgttgcgtgt aaccgcggac aggttcacct ccgcccaggc cggacggtcg atcatggtct
     9121 cctcctcggg gctccagtat ggcacggacg cggaaattct tggaggggga atcccccgga
     9181 aggcatcggg agagccgcat ttttttcgta aagccccggt ttttcccggt cgatactttg
     9241 cttggagacc agttctccgg aacggcatgc gcgcagacgt ccaggcatgc cgttttttta
     9301 tgcccggagg gaatctccat acctttgtct gttcggcacg ggtcctgctg cgggaagccc
     9361 ggtttcgctc ggtcctcgcc gcttcgcggc tgcggaaacg ctcaaccggg cttccctccg
     9421 cacccgtgcc gaatcaatga acaacggaga tccccacccg gcctaagcct tccaaggatc
     9481 tcggtccgcg cttggcctcc ggcttggagg cccgcacggc ccgcgttgac ggctttccat
     9541 ccatccccta cacttgcctc cgcaaggagg agcccgtgga tcggcagacg atacgcctga
     9601 cccagctgtg ttccgccgcg gggtgagcgg ccaagatggg ccccgaggcc ctggcgcagg
     9661 tcctgcgtcc cctgaccgcc ctcgtacccc ccgagcgtaa ggccgacctg atcgtcggcc
     9721 tggccgaggg ggacgacgca gccgtactca aactcgacgc cgagaaggcc ctggtcttca
     9781 cggtggactt tttcacgccc gtggtggacg acccctacga cttcggcgcc gtggccgcgg
     9841 ccaacgccct gtccgacgtc tacgcgacgg gcggtgagcc cctcatggcc ctgaacgtgg
     9901 cggccttccc ggcggacctg gatccggcgg tgatcgcggc cgtggtgcgg ggaggggcgg
     9961 aaaaggcggc cgaggcgggc tgcgcgaccg cggggggcca tacgatccag gacgcggagc
    10021 ccaaattcgg cctggcggtc atcgggctgg cccatccgga ccggctcttc ctgaagaccg
    10081 gggtccgccc cggggactcc ctggtcctga ccaagcccct gggcaccggg gtgatcacca
    10141 cctgggccaa gaacggagcc gtcgatccgg cggtcctggc cggggcggtg gagtccatga
    10201 agcggctcaa ccggaatgcc gcccgggtat tcgcccgctt cggggtcaag ggctgcacgg
    10261 acgtgacggg cttctccctg gcggggcacg ccctggagct ggcggagaag agctccgtcc
    10321 ggctcgagat cgacgcgggc gccctgcccc tcctgccggg ggcccgggcc gcggtggagg
    10381 ggaagttcgt gcccggcggc gccaagcgga accggtccta ctacgaagac cgagtcacct
    10441 tcgcggatgg ggtggactcc gtggtacgaa ccttgtgctt cgcgccggaa acctccggcg
    10501 gcctcctggc cgccgtgccc ggggacgcgg aagggtgcgt ccgggaactg cgggacctgg
    10561 gggtcgaggc ggcggtgatc gggcgggccc tgcccgcggt agacggcgcc gtcccgggga
    10621 tcgggttccg ctaggagcct gccgggctcc tggcggtctt cccgggcctg gaaccaggta
    10681 catcgacatc gaataaaaag attaccagct gaggttgttt caaaactcag ggagttttga
    10741 aaaagccgcc ttgaaattcg cgataggccc agcggctgca acgcaaccgc aagcaatcgc
    10801 aaagccaatt ccgaaggtga ccgacttttt gaattggctc agctgctcgt tcaggaagag
    10861 gcactcctca atccggatac agtgcccgca cctcgatgat ctcgtcccgg gattgaagga
    10921 aggccgaaac gagcttcgga tcgaagtgcg taccggactc cgagcggata agatcgacgg
    10981 cgagttccgg aggccaggcc ttcttgtagg gccgctcggt cgtaagcgcg tcgaagacgt
    11041 ccgccacggc gatcactctt cctatccagg gaatcctttc cccggagagt ccttccggat
    11101 atccggagcc gtcataccgt tcatgatggg tgagcgccgc ctccgcggca tggcgggcga
    11161 tctccgaatc tccctttccg atgatgtttg cgccgatcct gcagtgcatg cgcatgaggc
    11221 cccactcttc ctcgttcagg ggtccgggtt tccgcagcac cgcgtcgggg accccgatct
    11281 tgccgatgtc gtgcatcgga gccgcgttga gcacaagttc ggccgtcgcc gcgtcggcac
    11341 ctgccgccga cgcgatgatc tgggaatacc ggctcatgcg caggatgtgg gctcccgttt
    11401 cattatcccg aaactccgag gcccggacga gtctccggac cacctcgaga tgggagtccc
    11461 ggagttcctt cgtgcgctcc tcgaccaggg actcgagctc ggcccgcgag cgggagagtt
    11521 caagttgggt tcttatgcgg agctgaagta tgcccggcgc atggggcttc aagagaaaat
    11581 cggaagcgcc tcctcggatc gccgcatcga ccgcgtttcg atcgaagaac ggagccgaaa
    11641 agataatcgg gactcgctgt atcgtctcct ctttctcccg aaaccgcgcc aggagggtca
    11701 agggatcgct gccccagaac agggcgtcca ggaggacgac atcgggtcgc gggtcgccga
    11761 aggagtactc ccagaattcc cggtcgttcc tggcgtagat gaactcgtac tcgggcccga
    11821 gcgacttgca taggagttcc gtcgccggat cgccgtccgc gatcaggatg cggggtttgg
    11881 cgttcatcga cctgtacctg gtattccgtg tcatcccggg ctacacgggt cttcgtcccc
    11941 gagcggacag atcgtccacg atcggatcgc cctccccggc cggggctgga gagtcttcaa
    12001 actgcttcct gaggggagca tacacccgtt ccaccgtctg tgcatccatt cccaggagga
    12061 cggaggaaaa cctcacgagg gccttgatcc gtcgggggat gctcggctct ccggcgcccg
    12121 gggacccgag gaattgggcg aatgtgaggg taagctccgc gaattccaag gcgtgctcga
    12181 cccggcaccg gcccgcggcg ttgccttgtt ccaggatgcg ggcgaaaggg ggcgcgcaga
    12241 actcgaccat cgccagcatc gtcttgtccc ggatgtctat gtacttgatc ttctgcagtt
    12301 tggcgtacgt ggcaccgatc aggttcgtca tgatcgcgac ggcggcctga tacctgtctt
    12361 caaacccgag gtcggagtcc agttccaccc ggatgagctg ctcgcgcacg gcggcggctt
    12421 cccggtccac gatggcgtcg atgatgcctt ccttggaatc gaagtggtaa tacagtgtcc
    12481 ccttcgcgac atccgcggcc gtcgcgatcg agtccatcgg agtggactcg aagccgttgg
    12541 acctgaacag ctccagggcg gtatccagga tggtgctacg agtattcatc gtccgttccg
    12601 tcatgggttt ctccgttttg ctcgggaaat catataggtg agaatgaaat tagtcaacat
    12661 attgactgac ggtcggtata atattatact gaccgtcagt gcaatggagg tgatacatgg
    12721 atgcaattcc gcggaccctg cttctcgtgg aggacgaatc gatcatcgcg ctcgcggagg
    12781 ccagaaccct gagctccgcc ggctattcgg tcatccacgt cggttccggc gaggaagcca
    12841 tctccgcgat gaacggagag cgggggtcgt cgatcgactt gatcctgatg gacatcgatc
    12901 tcggggaagg catcgacggc acggaggccg cctacgagat cctgaagcgc cacgacattc
    12961 cgatcatctt cctctcctcg cattcggacc cgaaaaccat cgaaaagacg gagaagatca
    13021 ccaactacgg gtacgtcgtc aaaaacctgg ggcatacgat gatgttcgcc gcgatccgga
    13081 tggcgttcaa actccatacc gcccaggcga agatacggga acgggaggag tggatccggg
    13141 atctgatcga caaaatcgag gatggaatat gcatcctgga cgagaaggac agattcctct
    13201 acgcgaactc ggcgctctcc ggcgtctttg gagtcgagca tggacggctt gtaggtctgc
    13261 ccctctccac catggtcggg gaaagcgccc tagaaaccat ccgggcgaat ctccagcttt
    13321 cggatgtccg acccgtcggg gagacgagcc tcacggtgga gggcgccgac ggaatatccc
    13381 gcgcccttca gctgaagatc atccaggact acgacgccaa gcacaaatac aggggttccc
    13441 tggtagcggt ccggctccag gccgccgagt ccggaaccgg cgcgcccgcc ggggatcggg
    13501 gcacggtgcg gaaccgcaag gcccgggatg tctatgtcca cgaattgctc caccggatca
    13561 agaacagttt gaacataatc gtaagtctgc tgagcctgga agcccagcgc gtgtcggatg
    13621 aacccgcccg gaggatgctc gtcctgacgg agacccgaat ccgatcgatc tccctcctgt
    13681 acgacttcct ctcccattcc acgggggagg atcggatcga cagcgtagaa tacatccagg
    13741 agctgaccag gctcctgaag gagacatact cgagtcccga cagtcgtatc gagttcatca
    13801 cgagcgccga tccgtttccc ctgtcatcga aggtctgctt gtcgatcgga ttgatggtca
    13861 acgagctcat cagcaacgcg ctgaaatacg gattcccccg gggccgggag ggtacgatcc
    13921 ggatcgagct gaggaaggag gcggactccc tgatcctccg ggtccgggac gacgggatcg
    13981 gcctgcccga ggattatttc ccgaacgaga atctcggatt cgggttgcac ctggtcaaga
    14041 cactggcgtc ccaactgaac ggaaccatga acctggttaa cgaccgcgga gtctgcgtga
    14101 cgatcaccgc ccgggtccct caggaagcga cggacgccgg cggtccgggc gagcagaagg
    14161 tcggcgcgtc cgcgtgacct ccgcgccgta aggatcaaag gcccgggatg atcacgtagc
    14221 cgatagcgcc gtccgctgcc gtggtgatcc cgggccgttt ccttgaatat gtctgaagga
    14281 attacccgac cgctttcctc ataccgcctc gaccaaatcc tcggagttcc cgacccgatc
    14341 cgcgctgaat acccgatcga catccagcac caacacgaaa ccgtcccggc ctctcccgat
    14401 cccgacgagg tactcggagg gtcgtacggg gccgaagcgc ggcgggtcga tcatcggcgt
    14461 cgagaaattc gccacgccgt acacggcatc cacgacggcg ccggcgagaa acgccccgtc
    14521 gccctttccg acttcgagga taaggatcgc ggcgctttcc ccgacgggcg gcaccgccat
    14581 gccgaggacc agcccgagat cgaccacggg gatacaggtt ccccgcacat tgatgattcc
    14641 ttgagaggag tcttccttcc cgggcaggcg ggtcaagggc gagtattcca ggacttcccg
    14701 gacgttcttc acgttcaccg cgaaccggac ttcgtgtatg gagaaggtga ggtaggatgt
    14761 accgctgtcg cattccatct tgcatcgctc cctgtcgacc gtcaaaactc ttcgaactct
    14821 tccccggtga caccggaggc ggccatggtt ttccgtacgg cgatcgcggt cgaagacggg
    14881 cggccggtcg ccgccggccg tggagccgcc ggaaccgttg caatcctcgc gggctgggcc
    14941 ggcctcgctt cggctgcccc ctcgacggaa tacaccattc ccgcctccga aaacttgaag
    15001 aacccgatcg cctcggacag gctgttcgcc tgccccgcca actcgtccgc cgtcgcggcc
    15061 aattcttcgg agttcgccgc gttctgctgg atcaccaggt cgatctgggc cgcgcccttc
    15121 gacatctgct cgacgccgct cgactgctct tcgctggccg cggctatctc ccggatcagg
    15181 tcctcggtct tcttgatgtc cggcacaagc gcctcgagcc gcgcccgggc ctcgtcggcg
    15241 acggccactc cgtcgctgga aaggccgttg atctcggacg ccgcggtggc ggaccgctcc
    15301 gccagcttcc ggacttccag ggcgaccacc gcgaatccct tcccggcctc gccggcccgg
    15361 gcggcctcga tcgccgcgtt cagggccagg agattcgtct ggcgggcgat ctcctcgacc
    15421 accccgatct tcgaggcgat caccctcatg ctctccgagc tcgtagtgac cgaacgttcc
    15481 gcatcctccg cgttctgtcg cacccgggcc gcgagggcct cgatctgccg cgtggactcc
    15541 gtgttctgtc cggcgctggc cgccaactcc tccaccgacg cggacagctc ctcgacgctc
    15601 gccgcctgct cggtcgcgcc gctcgacagc ccctgggcca tttccgagag ctggcccgat
    15661 ccgctcgaga cctgaacggc agcggattgg atctcctgga agaccgatgc gagcttctcc
    15721 cggagctgtt ccatctcctt cgccaacatg cccaattcgt cggtcctcga ggccgcgcga
    15781 tccaggagca gacgttccgg ccctccaagg tagagttcgc cgtcggacag gtgctccatg
    15841 gaggagacga tgatcttgac gagattgacg atgattcggg ctatcagaag tgccaggagg
    15901 aacgccaggg caagggagca aagcgtgagg atatacagaa ttctggccaa ggacgtcgag
    15961 gtttcttgaa tctcgctggt gggaatgctc agtccgaaac tccagcccgg cgtgttcggg
    16021 atacgtctga aaaaaccggt cacctccgtc ccatccggga gaaagtacct tccgttgccg
    16081 gaatcctcgg acaacgcccg cttgccgaaa tcgtccagag ccttgtaccc ctgcttgtcg
    16141 gcgtcggaca agctcaacgc catgaccgcc ttgggattcg gatgggcgat cacgagtccc
    16201 gtatcgtcga ccacccagcc atacccggat ttcccgatct ttatgccgct tgtgatggtc
    16261 gacagggtct ccagcttgaa ctggaagcca aggatccctc tcgtattgcc ttcagggtcc
    16321 tggacggcga ctacgggaac gacgatgggg aggttgatgc tcctggaaat cacgggcctg
    16381 ccgatgaacc tatccttccc cttgcgcagg atcgcgtcga agtaatctcg atcctgtaca
    16441 ttggccctcg cgccgtccgc ggtgacggat tccccgtccg gccagacata gaacacggtg
    16501 actacttccg gcgacagctt accccggaga ccgaggatcg cggactcgac ggcttcccgg
    16561 tctccccgct tgaggacgtc ctgttccgcg atcatcctct gctgccaaaa cagtttatcc
    16621 atcatctcgg agagctggga gacgcgagcg tcgacgatct gccggttata ggacagcgcg
    16681 agatcgttca tgctgtcctt catccggatg cttatcagga tcgtgatccc tccgatcatg
    16741 acggccagga atgcgacgat gatcagagcg attcgcacgc cgaaggacac acccttgttc
    16801 atttccggct cccggttcca cgaatcaata tattgggaag gatgtggaac gcggcatttt
    16861 atactgacgg tcagtttatg tcaatagcta taccgaccgt cagtatattt aagaacagta
    16921 aaacggattt gctggatgga ttagacagga aaacaactgg ttggataagc ttcgagcgga
    16981 agaacggtga aggccgcgcc cgcttcggct acgtgccgtt ccgggcgatg cggtccaggg
    17041 cgactgcggc ggcttgccta agcacccccc ccgggcgcat actgtcccca tgcagaacga
    17101 cgccatccgc atcctcatca ccggcgggac cttcgacaag cactacgacg agatccacgg
    17161 cgagttgacc ttcaaggaca cgaacctgcc ggagatcatc aagcagatac gcaccaccat
    17221 ccccgtggag ctggagatca accagctcat cgactccctg cagatgcagg aggagaaccg
    17281 cgagcgggtc ctgggggcct gccggcgagc cccggagtcc cggatcatcg tgacccacgg
    17341 cacggacacc atggccgtga cggcccgggt cctgggggag gccgggctgg acaagaccat
    17401 cgtcctgacc ggggccatgg tgccctacaa ggtctcgggc tccgatgccc tgttcaacct
    17461 gggcaccgcc ttcgcggcgg tccagctgaa accgccgggg gtctggatcg ccatgaacgg
    17521 ccgggtcttc ccctgggaca gggtgcggaa gaacaaggcc gggggcgtgt tcgaggagat
    17581 ctgagggtgc cgaaatcccc gtccccgggc aggcgagccg ccgcgggccg acgcacctcg
    17641 ggcgcggccc gagcggccgg cccggggcgc ggccgccgct ccccctcccc ccgccccgga
    17701 aagccccacc ccgtgctcta ttccgccgcg gctgtcttgg tcctggccgc cctggcgggg
    17761 gcagcctgga tcaccggggc cttcccgggc cgcgaatcgc ccgggccggg cgccgcggct
    17821 ccggaggctc tccgcccttc ggattcggcc gcgccatccc tcgaaacccg gtcctacggc
    17881 gcggaagccg ccccggaacg gtctcccgtc ccggaggcac cacccgagcg gcccgccgga
    17941 gggacggtcg tgcggaccgg cgccggacgc agccccgggg gcgtcccgga gcggccgaaa
    18001 cctccgaagg ggaaactcgt cctggtcatc gacgacgcgg ggcacaacct ccgggacctg
    18061 gagcccttcc tcgccttccc cgggcccctc tccatcgcgg tccttccccg cctgcccttc
    18121 tccgaggagg cggcccggcg ggccaaggcg gcgggcaagg aagtcctcct ccaccagccc
    18181 atggaggccc tggggggcga ggatcccggc ccgggggccg tccgcctggg catgggagac
    18241 ctggagatag ccgcgctcat agcggagaac ctggccaccg tgcccggggc cgtcggcatg
    18301 aacaaccaca tgggttccgc ggtgacccgg gaccctagga tgatggaggc ggtcctggag
    18361 gccgcccggg cttcggggct ctactacctg gactctttaa ccgtttccga ttccgtgcta
    18421 cactccgtcg cctcgcgact gaacctgagg acctgggaga gggatgtgtt tctggacaat
    18481 taccctgaac gcgcggccat gctcaagcag ctggacgacg gcatcaagcg ggcggagaag
    18541 aagggccttt ccgtgatgat cggccacgtc tggtccgcgg agctggcgca gacccttatc
    18601 gacctctatc ccgggctgat cgagcagggc ttctccctct cgacgatatc ccgcatcatg
    18661 ctcgaggatt cggatgagga ttctggggat tgagacctcc tgcgacgagt cctccgccgc
    18721 ggtggtggag gacgggcgcc gcatcctctc caacgtagtc gccacccaga tccccttcca
    18781 cgccgagtac cagggggttg tccccgagat cgcctcccgg aagcacgccg agtggatctc
    18841 ccgggtcgtg gcggaagccc tgacggaagc gggtgtctcc gccgcggaca tcgacggggt
    18901 agcggtgacc gccaagcccg ggcttgtggg ctccctcctg gtgggggtct ctttcgccaa
    18961 ggcctgggcc tgggccctgg acaggccttt cgtgggggtg aaccacatcc tggcccacct
    19021 ctacgccccc cttctgtccg aggacgtgga ctaccccttc ctggggctcc tggtctccgg
    19081 aggacacacc ctgatctgcc gggtggacgg cttcgacgac atttccatcc tgggcacgac
    19141 catcgacgac gcggtggggg aggccttcga caagaccgcg aaattctacg gcttcggcta
    19201 ccccgggggc ctggccatcg accgcctggc ccggaagggg gatcccgcgg cctgccgctt
    19261 cccctccccc aacctctaca agggcgagca ccgctacgac gtgtcctact cgggcctgaa
    19321 gaccgcggcc atccgccagc tggaccagtt ctgggatccc gcctaccctc ggaccccgga
    19381 gaacatagcc gcggccttcg agaaggccgc ggtggacctg ctcctgtccc gtctggaacg
    19441 ggccgcccag gacacggggc tctcgacggt ggtggccggg ggcggggtgg cggcgaacac
    19501 ctacctccga gcgcggctgg ccggacggac ggacctgcgc gccctcttcc cgcccctccc
    19561 gctctgcggg gacaacgggg ccatggtggc ggggatcgga ttcaagtacc tggagcgggg
    19621 ggatcgggac ggcctcgatc tgaacgcctc gccccgggtt cccatcttcc ggcgcctgga
    19681 caagcccggc cgggctcggg actagggcgg tccggctccg cgcgggagat ccggagccca
    19741 gcctcaatct accaaaggtt tgccccggga gaccttccgc accgccgtga gccgggtctt
    19801 gagccccgag cgtcggaaca ccgcgtccgc cagccacgcc gcctcctggt ccgcctcgta
    19861 ggcgatcacc aggaagcgca gggtcgccgc gtcggaagtc gccggcccct ggaacccgaa
    19921 atcgtaggcc cggaagttgc cgggcaccag tttcacctcg
//
LOCUS       DAOTEI010000048        19878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1788_length_19878_cov_4.871816, whole genome shotgun sequence.
ACCESSION   DAOTEI010000048 DAOTEI010000000
VERSION     DAOTEI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1788_length_19878_cov_4.871816"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="P5313_06585"
     CDS             <1..279
                     /locus_tag="P5313_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80062.1"
                     /translation="ILVTHQGYDADLAMAARLVAYMAKWNSLHPVWRVVDALINPVMF
                     RLNRAIYRDRIVNFRQGLLTGIVVLFGLRILGGILTGLLAGLLSRLPF"
     gene            complement(343..2532)
                     /locus_tag="P5313_06590"
     CDS             complement(343..2532)
                     /locus_tag="P5313_06590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY80063.1"
                     /translation="MSPITPGRLRRLRDTWYLFTVLNAFSFSLLSGSVITLFALSLGA
                     SGTVVGALNAFGFATFFFMPLGRILISRIRIVDAFGWGWVLRYVFMLPVLAAPFLADQ
                     GRADAALFLVLAATAGFNVFRGIGLIGNNPVLALLGGDRDRGAFLSNVQIINSLVSIA
                     TYLAVALLLGRSASPARYALFLGAGIAAGLVGCLLLFRIPEPEEYRPARTSSWPAAIR
                     EAVRSKPIRDYFLVFAVVSFVAGTARTFLVVYPRAVYAAGDGDVMLYTLVSSLGAVAM
                     GLLTRKLVDRLGAKPLYGIFTAVSALSILPVLVSPGFASPVPVFLFLAAVNFTAGFGL
                     AGQENAAQTYYFALIPVSRTLDLAVIYYLVYGLAGALGSAAGGLVLDGLSALGISPAL
                     SYRILFAGLFLLLAAALARTPALVRLGASSVRESLGILFSLRDLRAIGLLDRLDRSSR
                     PDEEIGIIREIGLSGRPVAQRELLPYLRSPRFDVRMEALTALGNLRDLSPPTLEAVAR
                     ELRGHPFTTAHLSARILGKAGDPSAVPILREALASEDYMLRGAALVALGRLGDGPSVP
                     AIEEIVASTDNPRLVIQGAYALELLGSSASVPVLVEVLRKEGTPDFVRDEAVLSLAAV
                     LGVFDRFYPIYGEWTADPDRGRALLQDALEESAGSGSEAARERGRAVEGMLSDPPRGA
                     EAARVILGTESLDPRAAAVLAEAAVDPTLSGHPGFRFLLAVLAALAA"
     gene            complement(2529..3899)
                     /locus_tag="P5313_06595"
     CDS             complement(2529..3899)
                     /locus_tag="P5313_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRY80064.1"
                     /translation="MRNLTEGSETKEIILFALPMLIGNVFQQLYNMVDSIVVGQFVGK
                     QALAAVGSSFAVLFLMIALIMGVTMGFTILIAQYFGARDKAKVRAVVDTAYIVLFWAA
                     LVLSVVGVLAVPLILKVLRVPADVAPEAGTYLRILFAGSLATFGYNGVAAILRGIGDS
                     KTPLYLLIAASFLNIALDLLFVIAFGWGVAGVAWATIISQAASFLGALVLLNKRNEYV
                     RLSVKELKFDPEMFRLSLKLGIPSGIQQTLVAAGMMVLTRVVNTFGTDVMAGFSAASR
                     LDTFAMMPTMNLGMAVSTFTGQNLGAGKPERVRRGHSSALLVGLGISVFIGVVMVFGG
                     PGMVRLFTRDPEVVRIGARYLLIVGAFYSLFTIMFVNNGVLRGAGDVMIPMVNTLLAL
                     WIVRIPTAVALSSRMGPDGIWWSIPAGWLMGAVFSSWYYRTGRWKRKAVVKGPVEPAP
                     AEAAST"
     gene            4012..4716
                     /locus_tag="P5313_06600"
     CDS             4012..4716
                     /locus_tag="P5313_06600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY80065.1"
                     /translation="MDEPNRAADCAFFSDLSRTADFYLIRHGESEGNARLIMQGRLDL
                     PLNPRGEAQAAAAGAWLADKGVTALFTSPLRRAYRTAEILAAEAGLPGPRPEPVFTEL
                     DTGIFTGLSVEEIRKKHPGIYGEFRARSWEAVPGAESSASLYDRALRGWGFLKDRVRE
                     TEGALAVVTHGGCLQWMVRATFGCRTWMPLLTTGNSGIFHLSVHPHPEGRPAYLQWKL
                     LNHQPGFDVSATPPVF"
     gene            complement(4744..5484)
                     /locus_tag="P5313_06605"
     CDS             complement(4744..5484)
                     /locus_tag="P5313_06605"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY80066.1"
                     /translation="MPQGFEILFEDDGILAAAKLGPLPVQADATGDPSLQGLLTALLS
                     ERDGRPAFLEAAHRLDRRTSGIVLFGKSRRAVSALDAAFRGRRIRKLYWAVPDRAPPI
                     PEGRLAHRLVRDPRSNRTLAVPLKGRSELWSSKPGTPIPPEALNPDLAVLDYRTLGHG
                     DRYELLEVRPWTGRTHQIRAQLAAAGFPIRGDLKYGARRSCRNGLIMLHARAVEFEHP
                     LTGEPIRLTAPCPLDEPLWRAFPEEALP"
     gene            5627..6334
                     /gene="deoD"
                     /locus_tag="P5313_06610"
     CDS             5627..6334
                     /gene="deoD"
                     /locus_tag="P5313_06610"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRY80067.1"
                     /translation="MSIHIEAAPGQIAPDILLPGDPLRAKYIAETLLEGAEQFNRVRN
                     MFGYTGTWKGRRVSVMGTGMGQPSLSIYVNELLREYGVKRLIRVGTCGSLRKEIAVRD
                     LVLAVSACTDSAVNRIRFRGMDYAPAAAFPLLLAAYQAARTRGFSVQAGPVISSDSFY
                     TEDPDQWKLWAKFGVLAVEMETAELYTLAAKFGAQALSVLTVSDNLVTGGNTTSAERE
                     TTFRAMAELALDTLTVG"
     gene            complement(6561..9773)
                     /locus_tag="P5313_06615"
     CDS             complement(6561..9773)
                     /locus_tag="P5313_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80068.1"
                     /translation="MLFDPGNLITLAIVAIVLAVYRFLDRDNRSLEKVKKFTDRIRED
                     LAAYVDQRSEDLKSYGIELDVRSKAAKEVLKRVKEAQDELESRSDSLDAMGTRLAEYD
                     GVLARLSDMTGRVDENLKRIHEESLFADQLARKLKDSEEALTRIDAEVPLLRERFAED
                     ARKALEAAREGIVEEARSRLDATVEEVEALREETEQRREQVALFYREANDQAKARYVE
                     MEKLLSSAFRKAQDEAERLEDSTYEKFREQIETRGERLKEALDSKLKAIQEAAKERVL
                     ETQGLVKGFKAEWQQEARGLLDAARSEISGQTELLAARIDELDTRAEKAEGLASARFD
                     KIEARALELAQALQAKIKDQLKAHQDELAARQAELRAELAKYDASGKEMEKEIRSVLS
                     GIHEKMVREAAALEKKTFEDFGRRVEEYRAEVEYKFERLSESNVDIQKMEETLRAAMA
                     GTEKRVEEDFALFGKDLAARQSAFEGEILAEASRVRGNMEAVEKEINVLKASAYQSVS
                     EKLKVFEDEFFADLKARSENIERRLAEWQGDLDTRLAALSEESQTANRETAKAAAEEL
                     RVALGSLQASLFDQMGKLSAQAQAFRDGITDQMESADGSLASLRDSVRAELAEAKAAA
                     DAVVRAELERFQVDGREKIKEAARDLETRLRALSESADQASREGKEFREALARDTQAA
                     QAEAARFRQELTQKIAQALEGFGRSYEAMSADSQRRSRELAAEMDQALQAFRRSAEDY
                     AGKTETEAKRFAERFERESGERARTLADMDARVKAFTAQTKLFERADELQNNLRVTIE
                     TLKGDLARLEARKGEITELETQFQRIKRLEDETDQKVSRFLAEKRRIDTLEEDFKRLL
                     GLSQEVDRKLASVTESRDSLTDLQTEFRRLSGLAEEAGTKFDRLEKKSALLDTTADGV
                     DKSFEALRGIEQEVKDLDQRVRPLADRMAELRAALDSLDKDRDRVDQAVSRLAGLDKD
                     LEETEARIEKVQKAREWLARTETRLEELNKQAQDQLKLLGTLLKQEAGAAKKETGAPS
                     LSTQETVRKLAHQGWKVEEIARAVKLSRGEVELILELSSQ"
     gene            complement(10104..10919)
                     /gene="proC"
                     /locus_tag="P5313_06620"
     CDS             complement(10104..10919)
                     /gene="proC"
                     /locus_tag="P5313_06620"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRY80069.1"
                     /translation="MDTSDSSRTFGFMGAGRIVEILLDSLGPAIGEGRGLIWNRTRPR
                     AERLAERHGLLVQESPEDLARNADRVLLAVKPKAVASVLTQAAPGMSPDTVLISMAAG
                     VSIARLRSFFATPPRIVRIMPNIALSVGLGMTAVCADEGVSPEEVEEILGIFRRAGRA
                     RLVEEEWLDTVTALSGSGPAFVFLFIESLADGAVLNGMSREAAYEFAAQTLQGAARII
                     LESGMHPGELKDIVASPAGTTIAGIHVMERRAFRSIVMDAVDAAARRSREISA"
     gene            11071..12726
                     /locus_tag="P5313_06625"
     CDS             11071..12726
                     /locus_tag="P5313_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80070.1"
                     /translation="MSDEIDPEIAALLGTDTGPKGPAERKSAADTPDFDSLFGGAGVV
                     MEERNKAEFDVDLTRARIAPVTKDQESVPNDFFSDPDFYRKALAAEGEESQKLHALLS
                     KYLQTKDPKDRGVYRQQLIPAYWNLLHRVALKCASPGFPIAKQVLLRYGVLLPTLLSP
                     EQQDMIQRVVHKKELDEPFYYMDEWLRAVALGHVGQSATDEVKSRSGDDRARFNAILQ
                     KVQGKKETAESLMKAKAETRRTLEGLLREKLDLLAQHDSVPGFYHVPAPYTEAQKKVM
                     SEFQEVFRAMLQADKELGSSIEAFKDATAELESVRQKMDGLEEDVKANFQALAQEFET
                     ARQMTKLCVGRQGNHFPVLTREYFHGGSRDVGSRENVARLLAWIEGIDTQAFCRPYKN
                     TLNRIVPYVVILPTYGDFGICWEPFDKFNRATSRGRLAVPLYPKSLQLAVVTAVADLR
                     WQVAKEKASYYWMEEGLTGNYYQWFTSKKMKGDVKEFFIQDYILWITKESEGTQKLDK
                     EVRGVFWRYMPFAQEIKDKLKTRSYVYQELYQKDINRSMSDGY"
     gene            complement(12698..13585)
                     /locus_tag="P5313_06630"
     CDS             complement(12698..13585)
                     /locus_tag="P5313_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY80071.1"
                     /translation="MAVSRRLAVLSSLTASFIWGFTFLSIKVALGALPPMSLGLARFA
                     VAGILLAGLLAARRRPPRLDRADAARMAASGLLGVTAYFFFENNGILRISASEASLMT
                     ATIPVFTLLAESAFLRTRLSASKAAGAILSAAGIGLVVAESFRFSRSPAGYLYMLLAA
                     FAWVGYGFLTRPLASRYDRLEITFWQSLFGMAGFVPFALFETVNWAGFGWGVGLNVLF
                     LGIFGSALATVFYVTGLEVLGPGVVNVHINLIPVVSVAASFALLGERLSPLQAAGGVV
                     VVGGVYLATGISTRRTSSG"
     gene            complement(13588..14385)
                     /gene="mazG"
                     /locus_tag="P5313_06635"
     CDS             complement(13588..14385)
                     /gene="mazG"
                     /locus_tag="P5313_06635"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRY80072.1"
                     /translation="MSSPDAFERLHGIVRTLRSPEGCPWDREQTPESLRGNLLEEAYE
                     LVEAVDEKDPAHVREEAGDLYLLTTMIAHMYEERGLFTVADCLDSISDKLIRRHPHVF
                     GDSEASTPDAVIRQWNEIKETVEGRRPKDSVLDEVSRALPPLERAYKLQKKAAKAGFD
                     WTDVRDVFAKLREEIAETEEARETGDRPALEAEIGDILFSAVNLSRALGVDPSVALHG
                     AVERFSRRFRHVEKGMAREGLPLSADNMARMDELWDEAKAGEGRPAR"
     gene            complement(14478..16304)
                     /locus_tag="P5313_06640"
     CDS             complement(14478..16304)
                     /locus_tag="P5313_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80073.1"
                     /translation="MRRILIPAAVAVLVLASCVSSGGDASRLVPSDAYAALVVESPAS
                     LFRSAEDFVREAGLDRFTKGRTLAELLREEAGGKEGFREASEVLDFGRPLVLSVLPDG
                     NGAEGSPQAVLWLPLKKEKGAFERLRAALTGFEGEAAFVEGYAVLGLKGPAPSALPKK
                     TADLSRLSAYPAGSVKAWINVEALRADFQDRWDSALKDAFGNGDEDGEPGLELDEDPD
                     APYNYNERNFDWNEYEGETGGSGPMAGLLGDAADPEELLKKAAENLRTLDFAVGADGR
                     GFHFRAGAVPHPDRALGRLAAAAGPARGIPFLKYLEADSLMGGAASFDPRALADLAEL
                     YVKALGMEKLLGTEYFELLESSYAALGADSAFSFDLTVDPDFLQKAERAQSPGEISEL
                     LERSLAFEAAGAGTLRDREAYRSVLRNLGGAGLFGEAFRELLGSSGLTLDLEVSEGSV
                     EGIPYDSIRIDLGGEGLGSDPGARAVVDVLLDKLTFHAGYVKDRYLLVLGDPAKLPAA
                     VRRDGAARPLGGEASYKAFAADLPKEIRGAYYLSLKKIFGLAAAFSKDKGKLPSEGLD
                     RLYGYFAAAPGRLETGFFLGAGDIRALVGLVPEGREKGLDIF"
     gene            complement(16306..18504)
                     /locus_tag="P5313_06645"
     CDS             complement(16306..18504)
                     /locus_tag="P5313_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY80074.1"
                     /translation="MRSIQAKMLLLLIPGTFLLFTGAVAFLGYDTSGTITDYAYRAAA
                     QYAREGASLAERRFRESLNMFEDSARLAETLKREGRVDRAILPAVLLDTLRTHDSAFA
                     LWILFEPNAWDGRDSRFAGTLGPEDTGAFAVWAYRDEAGSATLSEEAWAEEAYGEEYY
                     TGPLARSGFWLSDPYEEEVEDGRTVRMITLARRLEDGNGGILGVAGVDFSLDFLEGIL
                     ADVDERSGGASTIANYAGLILADTLAEGQGSYLSDSHSADTVSAASELTRPDTARETA
                     VEAEIDGRAVLQMMESVRLHENLDPWIFIVSLPKEKILEIPRRIYLELTVAELAVLAL
                     LTALIVAVSRRISRPLVSLTGTFDVIASGDLRPAANVRGKDETGRLAAGFNRLTDTLN
                     GVLGDLKSALGTLEANAGDLAAEMERTRTALEGTDRSVETVVGRGTDIDREIKAVTAS
                     VERIQESARSLEARSRDESEMILQSVAAIEEILANIRAVTGSVVKASEQYGRLNESSA
                     RGEELLSEVIEKAQHILSRSEALLETNTVIANIASQTNLLSMNAAIEAAHAGEAGKGF
                     AVVADEIRKLAENAAEQSKAVEAMLGEVASAIRSIADSSRRAGENFGEIQSLIRTITR
                     LEEEVKAAMEEQSAGSNQILASLDRMKSTSGEMDRGAASVADLAELIAGEMKDLNRYS
                     EDIQVSIEAVRSNGGVLKSAYSRTSSLAENNQSNAARVADSLRNFRLKGE"
     gene            complement(18639..19343)
                     /locus_tag="P5313_06650"
     CDS             complement(18639..19343)
                     /locus_tag="P5313_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80075.1"
                     /translation="MLKEELIKKSPVRILERGIEGGLKSGNIGVIASRKGIGKTSVLV
                     QLALDKLLQGKKVVHVSFTTHTSYVISWYENIFTELTRKRNLENVEDIHDDLVKNRVI
                     MNFNQEGISTEQILRSLGAMIVDGGFKADSVIIDGFDFTRSAPDHLAKVKDFAARMNL
                     EVWYSCTMVGEEPLTDKRNVPVMLREFLDSISVLIVLEPKAEYIHFLVVKDHERMNPE
                     DLSIKLDAKTLLIAET"
     gene            complement(19447..>19878)
                     /gene="fusA"
                     /locus_tag="P5313_06655"
     CDS             complement(19447..>19878)
                     /gene="fusA"
                     /locus_tag="P5313_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257779.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY80076.1"
                     /translation="PIVNVRCVVNDGQSHPVDSSDIAFQQAAIGAFREAYEKAKPVIL
                     EPIMKVVAEGPTEFQGNIFASVNQRRGVIHASTEEASFCRVEAEVPLAEMFGYSTVLR
                     SMTQGKAEFSMEFLKYAKVPTSVSEQLRKDYLEKRRKEQGR"
BASE COUNT         3246 a         6672 c         6745 g         3215 t
ORIGIN      
        1 atcctggtta cccatcaggg ctacgacgcc gacctggcga tggccgcccg gctggtggcc
       61 tacatggcga agtggaactc cctgcatccg gtctggaggg tcgtggacgc cctgatcaat
      121 ccggtcatgt tccggctgaa ccgggcgatc taccgggacc ggatcgtgaa tttccggcag
      181 ggcctgctca cggggatcgt cgtccttttc ggcctgcgca ttctgggggg catcctgacg
      241 ggcctgctcg cggggctcct gagccggctg cctttctagg agcctgtcgg gcttgtgtac
      301 aaattgcgcc tctcggcgca atttgaggcg atcatcgggc tcctaggcgg ccagggcggc
      361 aaggaccgcc aacaggaaac ggaaacccgg gtgcccgctc aaggtaggat ccaccgccgc
      421 ttccgccagc accgccgccg cccggggatc caggctctcc gtccccagga taacacgggc
      481 tgcctcggcg ccccggggcg ggtccgagag catgccctcc acggcccggc ctctctcacg
      541 ggccgcctcg gagccggatc ccgccgactc ctccagggcg tcctggagca gggcacgccc
      601 ccggtccgga tcggcggtcc actccccgta gatcggatag aagcggtcga agacccccag
      661 caccgcggcc agggagagca cggcctcgtc ccgcacgaaa tcgggagtgc cctccttccg
      721 gaggacctcc accaggacgg gcaccgaggc ggagcttccc aggagctcca gggcgtaggc
      781 tccctggatg accagccggg gattgtccgt ggaggccacg atctcctcga tcgccgggac
      841 ggaaggcccg tcccccagcc ggcccagggc caccagggcc gccccccgca gcatgtagtc
      901 ctccgaggcc agggcctccc tcaggatcgg gacggccgag ggatccccgg ccttccccag
      961 gatccgggcg gagaggtggg cggtggtgaa gggatggccc cggagttccc gggccacggc
     1021 ctccagggtc gggggcgaca ggtcccggag attccccagg gccgtcaggg cctccatgcg
     1081 gacgtcgaag cgcggggacc ggaggtaggg caggagttcc cgctgggcca caggacggcc
     1141 cgacaggccg atctcccgga tgatcccgat ctcctcgtcc ggccggctgg aacgatccag
     1201 ccggtccagg agcccgatgg cccggaggtc ccggaggctg aagaggatgc ccagggattc
     1261 ccgtaccgag gaggctccca gccgcaccag ggcgggggtc cgggccaggg ccgcggccaa
     1321 caggaggaag agccccgcga agaggatgcg gtacgacagg gccggggaga tcccgagggc
     1381 cgaaagcccg tccaggacca ggccccccgc cgcggaaccc agggcacccg caaggccgta
     1441 cacgaggtag tagatgaccg ccaggtccag ggtgcggctg acggggatca gggcgaagta
     1501 gtaggtctgg gccgcgttct cctggcccgc cagcccgaag cccgcggtga agttgaccgc
     1561 ggccaggaaa aggaagaccg gaaccgggga ggcgaagccc ggggagacca ggacgggaag
     1621 gatggaaagg gccgacacgg cggtgaagat cccgtagagg ggtttggcgc ccaggcggtc
     1681 cacgagcttc cgggtgagca gccccatggc cacggccccc aggctggaga ccagggtgta
     1741 gagcatgacg tcgccgtccc ccgcggcgta gacggcccgc ggatagacca ccaggaaggt
     1801 ccgcgccgtg cccgccacga aggacacgac ggcgaacacg aggaaatagt cgcggatggg
     1861 cttggaccgg acggcctccc ggatcgcggc gggccaggag gaggtccggg ccgggcggta
     1921 ctcctcgggc tcggggatgc ggaacaggag gagacacccg accagccccg ccgcgatccc
     1981 ggcgcccagg aagagggcgt accgggccgg ggaggcgctc cgtcccagga gcagggccac
     2041 cgccaggtag gtggctatgc ttaccaggga gttgatgatc tggacgttcg acaggaaggc
     2101 cccccggtcg cggtccccgc ccaggagggc caggaccggg ttgttgccga tgagtccgat
     2161 gccccggaag acgttgaagc ccgccgtggc ggccaggacc aggaacaggg cggcgtccgc
     2221 ccggccctgg tccgccagga agggcgcggc caggacgggc agcatgaata cgtagcgcag
     2281 gacccatccc cagccgaagg cgtccacgat gcggatccgg gagatcagga tccggcccag
     2341 gggcatgaag aagaaggtgg cgaatccgaa ggcgttcagc gccccgacga cggtccccga
     2401 ggcgcccagg ctcagggcga agagggtgat cacgctcccg gacaggaggc tgaaggagaa
     2461 ggcgttgagg accgtgaata ggtaccaggt gtcccgcagg cgccgaaggc ggcccggggt
     2521 gatcgggctc atgtcgaggc ggcctccgcg ggcgccggtt cgacgggacc cttgaccacg
     2581 gccttgcgct tccaccgtcc ggtccggtag taccaggagg agaagaccgc gcccatgagc
     2641 cagcccgcgg ggatgctcca ccagatcccg tccggaccca tccgggagga cagggcgacg
     2701 gcggtcggta tccggactat ccacagggcc agcagggtgt tcaccatggg gatcatgacg
     2761 tccccggccc cccggaggac tccgttgttc acgaacatga tggtgaacag ggagtagaag
     2821 gcccctacga tcaggaggta ccgggccccg atccggacga cctcgggatc ccgggtgaag
     2881 agccggacca tgcccggccc cccgaacacc atgaccaccc cgatgaagac ggagatcccc
     2941 aggcctacaa ggagggccga ggaatggccg cggcgcaccc gctcgggctt gcctgccccc
     3001 aggttctggc ccgtgaaggt ggagaccgcc atgcccaagt tcatggtggg catcatggcg
     3061 aaggtgtcca ggcggctggc cgcggaaaag cccgccatga cgtccgtgcc gaaggtgttg
     3121 accacccggg tcaggaccat catgcccgcg gccaccaggg tctgctggat ccccgagggg
     3181 atcccgagct tcaggctcag gcggaacatc tccgggtcga acttgagttc cttcacggaa
     3241 agccggacgt actcgttgcg cttgttgagc aggaccaggg cccccaggaa ggaggccgcc
     3301 tgggagatga tcgtggccca ggccaccccc gcgacccccc agccgaaggc tatgacgaag
     3361 aggaggtcca gggcgatgtt caggaaggag gcggcgatca gcaggtacag gggggttttg
     3421 gagtccccga tcccccggag gatggcggcc acgccgttgt acccgaaggt ggccagggac
     3481 cctgcgaaga ggatccgcag ataggttccg gcctccgggg cgacgtccgc cgggacccgc
     3541 aggaccttga ggatgagggg caccgcgagc accccgacga cggacaggac cagggcggcc
     3601 cagaagagga cgatataggc cgtgtccacc accgcccgga ccttggcctt gtcccgggcg
     3661 ccgaagtact gggcgatcag gatcgtgaag cccatggtca cgcccatgat cagggcgatc
     3721 atcaggaaga ggaccgcgaa gctcgagccg accgccgcca gggcctgctt gcccacgaac
     3781 tgtcccacca cgatggagtc caccatgttg tagagctgct ggaagacgtt gccgatcagc
     3841 atgggcaggg cgaagaggat gatctccttg gtctcgctgc cctcggtcag gtttcgcatc
     3901 gtttatcctt catcgggtat ccgtccataa taaggaaatt tgaatatgat gaacagggga
     3961 gatccgtccg ggctttaccc cccgcccggg ccgccggtat actcccgacc catggacgaa
     4021 cctaaccgcg cggcggactg cgccttcttc tcggacctct cccgaacggc cgatttctac
     4081 ctcatccgcc acggcgagag cgaggggaac gcccgcctga tcatgcaggg caggctggac
     4141 cttcccctga acccccgcgg cgaggcccag gccgcagcgg ccggggcctg gctggcggac
     4201 aagggcgtca cggccctgtt cacctccccg ctccgcaggg cgtaccgaac cgcggagatc
     4261 ctggccgccg aggcggggct gcccgggccc cggccggagc cggtcttcac ggagctggat
     4321 acgggtatct tcacgggtct ttcggtggag gagatccgga agaaacaccc cgggatctac
     4381 ggggagttcc gggctcggag ctgggaggcg gtgcccgggg cggaaagctc cgcatccctc
     4441 tacgaccggg ccctgcgggg ctgggggttc ctgaaggacc gggtccggga gaccgagggt
     4501 gccctggcgg tggtgaccca cggaggctgc ctgcagtgga tggtccgggc caccttcggc
     4561 tgccggacct ggatgcccct cctgaccacc gggaattccg ggatcttcca tctgagcgtc
     4621 catccgcatc cggagggcag gcccgcctac ctgcagtgga agctcttgaa ccaccagccc
     4681 gggttcgacg tctccgcgac ccctcccgtc ttctgagggc ggaccttcac aagcttcggg
     4741 acactagggc agggcctctt ccgggaaggc cctccagagg ggctcgtcga gggggcaggg
     4801 ggcggtcagc cggatcggtt cccccgtcag ggggtgctcg aactccacgg cccgggcgtg
     4861 gagcatgatg aggccgttcc ggcaggatct ccgggcaccg tacttcaggt ccccccggat
     4921 cgggaagccc gcggcggcca gttgggcccg gatctggtgg gtcctgcccg tccagggacg
     4981 gacctccagg agctcgtagc ggtccccgtg ccccagggtc cggtagtcca ggacggccag
     5041 gtccgggttc aaggcctccg gcgggatcgg ggtgcctggc ttggagctcc aaagctccga
     5101 acggcccttc aggggaacgg ccagggtccg gttgctccgg ggatcccgga ccaggcggtg
     5161 ggccagccgg ccctccggga tcggaggcgc ccggtcgggc acggcccagt agagcttccg
     5221 gatgcggcgg ccccggaacg cggcgtccag ggcggagacg gccctgcggc tcttcccgaa
     5281 caggacgatc ccgctggtcc tgcggtccag acggtgggcg gcctccagga aggccggcct
     5341 gccgtcgcgc tccgaaagga gggccgtcag gaggccctgg agggagggat ccccggtggc
     5401 gtccgcctgg acgggcaggg ggcccagctt ggcggcggcc agaatgccgt cgtcctcgaa
     5461 gagtatctcg aaaccttggg gcatgcccca gggtaccacg gatcggatct ccgcaggaag
     5521 gccgccgatt ccgggtttac cgggcgggaa tcctggttta ctctggaagg cccgggaccg
     5581 gaaaactccc cggaccggac ctagtccaag ctcggaagga gccgatatga gcatccacat
     5641 cgaggccgcc cccggccaga tcgccccgga catcctgctt cccggggacc cccttcgggc
     5701 caagtacatc gcggaaaccc tgctggaagg ggccgagcag ttcaaccggg tccggaacat
     5761 gttcgggtac accgggacct ggaagggacg gcgggtctcg gtcatgggca ccggcatggg
     5821 ccagccctcc ctctcgatct acgtgaacga gctcctgcgg gagtacgggg tgaaacggct
     5881 gatccgggtg ggaacctgcg gcagcctgcg gaaggagatc gccgtccggg acctggtcct
     5941 ggccgtctcc gcctgcacgg actcggcggt caaccggatc cggttccggg gcatggacta
     6001 cgctcccgcc gcggccttcc ccctgctcct ggccgcctac caggccgccc ggacccgggg
     6061 cttctccgtc caggcggggc cggtgatctc cagcgactcc ttctacaccg aggaccccga
     6121 ccagtggaag ctatgggcca agttcggggt cctggccgtg gagatggaga ccgcggagct
     6181 ctacaccctg gccgccaagt tcggggccca ggccctctcg gtcctgacgg tcagcgacaa
     6241 cctggtcacc ggggggaaca cgaccagcgc cgaacgggag accaccttcc gcgccatggc
     6301 ggagttggcc ctggataccc tgaccgtggg atgaaaattt tcggaatcaa caacggagga
     6361 cacggagagc acggaggaag cggacgggaa accaaaggaa agacaggatg gacaggatac
     6421 agaatggatg aacaggataa tttcgtgaat tggctatctt agatcactat cctgtacatc
     6481 cttcttctat cttatgcatc ctgtctagat tttttctgat cctctgtttt ctccgtgacc
     6541 tccgtgatga gtcctgagcg tcactgagaa ctcagctcca agatcagttc cacttcgccc
     6601 cgggagagct tgaccgcccg ggcgatctcc tccaccttcc agccctggtg ggccagcttg
     6661 cggacggtct cctgggtgga cagggagggg gcgcccgtct ccttcttggc ggccccggcc
     6721 tcctgcttga gcagggtgcc caggagcttg agctggtcct gggcctgctt gttgagctcc
     6781 tccagccggg tctcggtccg ggccagccac tcccgggcct tctgcacctt ttcgatgcgg
     6841 gcctcggtct cctccaggtc cttgtccagg ccggccaggc gggacacggc ctggtcgacc
     6901 cggtcccggt ccttgtccag ggagtccagg gcggcccgga gctcggccat gcggtccgcc
     6961 aggggtcgga cccgctggtc gaggtccttg acctcctgct cgatcccccg cagggcctcg
     7021 aagctcttgt ccacaccgtc cgcggtggta tccagcaggg cgctcttctt ttccaggcgg
     7081 tcgaacttgg tccccgcctc ctcggccagg cccgagagcc tgcggaactc ggtctggagg
     7141 tccgtgaggg aatcccggct ctcggtgacc gaggccagct tgcggtccac ttcctgggag
     7201 agccccagga ggcgtttgaa gtcctcctcc agggtgtcga tcctgcgctt ttccgccagg
     7261 aagcggctga ccttctggtc cgtctcgtcc tccaggcgct tgatcctctg gaactgggtc
     7321 tccaactccg tgatctcgcc cttccgggcc tccagccggg ccaggtcgcc cttgagggtc
     7381 tctatggtga cgcgaaggtt gttctgcagt tcgtccgccc gctcgaagag cttggtctgg
     7441 gcggtgaagg ccttgacccg ggcgtccatg tccgcgaggg tccgggcccg ctcgccggat
     7501 tcccgttcga agcgctcggc gaaacgcttg gcctcggtct ccgtcttgcc ggcgtagtcc
     7561 tccgcggagc gccggaaggc ctgcagggcc tggtccatct ccgcggccag ctcccggctg
     7621 cgccgctggg agtccgcgga catggcctcg taggaccggc cgaagccctc cagggcctga
     7681 gcgatcttct gggtgagctc ctgacggaac cgggccgcct cggcctgagc ggcctgggtg
     7741 tcccgggcca gggcttcccg gaactccttg ccctcccggg atgcctggtc cgcggattcc
     7801 gacaaggcgc gtagccgggt ttccaggtcc cgggcggctt ccttgatctt ctcccggccg
     7861 tccacctgga agcgctccag ttccgcacgg acgaccgcgt ccgccgcggc cttggcttcc
     7921 gccagctccg cccgcaccga atcccgcagg gaagccaggg agccgtcggc ggattccatc
     7981 tggtccgtga tcccgtcccg gaaggcctgg gcctgggcgg agagcttgcc catctggtcg
     8041 aagagggaag cctggaggga tcccagagct acccggagtt cctcggccgc ggccttggcg
     8101 gtctcccggt tggcggtctg ggactcttcg gacagggccg ccagccgggt atccagatcg
     8161 ccctgccatt ccgcaaggcg ccgctcgata ttctcgctcc gggccttgag gtccgcgaag
     8221 aattcgtcct cgaagacctt gagcttttcg gagacgctct ggtaggccga ggccttgagt
     8281 acgttgatct ccttctccac cgcctccatg ttgccccgga cccgggacgc ctcggccagg
     8341 atctcgccct cgaaagccga ctgccgggcc gccaggtcct tgccgaagag ggcgaagtcc
     8401 tcctccaccc gtttttccgt gcccgccatg gcggcccgga gggtctcctc catcttctgg
     8461 atgtccacgt tggattccga gaggcgctcg aacttgtact ccacctccgc ccggtattcc
     8521 tccacccggc gcccgaagtc ctcgaaggtc ttcttctcca gggccgcggc ctcccggacc
     8581 atcttctcgt ggatccccga cagtacggaa cggatctcct tctccatctc cttgcccgag
     8641 gcgtcgtact tggccagttc cgcccgaagc tccgcctgcc gtgcggccaa ctcgtcctgg
     8701 tgggccttga gctggtcctt gatcttggcc tggagggcct gggccagttc cagggcccgg
     8761 gcctcgatct tgtcgaagcg tgcggacgcc aggccctcgg ccttttccgc ccgggtgtcg
     8821 agctcgtcga tgcgggcggc cagcagttcc gtctggccgg aaatctcgga ccgggcggcg
     8881 tccaacagcc cccgggcctc ctgctgccac tccgccttga agcccttgac cagtccctgg
     8941 gtctccagga cgcgctcctt ggcggcctcc tggatggcct tgagcttgga gtccagggcc
     9001 tccttcaggc gttcgccccg ggtctcgatc tgctcccgga acttctcgta ggtcgagtcc
     9061 tccaggcgct ccgcttcgtc ctgggccttg cggaaggccg aggacaggag cttctccatc
     9121 tccacgtacc gggccttggc ctggtcgttc gcctcccggt agaacaaggc cacctgttcc
     9181 cggcgctgct cggtctcttc ccggagggcc tcgacctcct ccaccgtggc gtccaggcgg
     9241 ctccgggcct cctcgacgat cccctcccgg gcggcttcca aggccttccg ggcgtcctcg
     9301 gcgaagcgct cccgtaggag gggcacctcc gcgtcgatcc gggtcagggc ctcctcggag
     9361 tccttgagct tccgggccag ctggtccgcg aaaaggcttt cctcgtggat ccgtttcagg
     9421 ttctcgtcca cccggccggt catgtccgaa agccgggcca ggaccccgtc gtattccgcc
     9481 agccttgtac ccatggcgtc cagggagtcg gaccgggatt ccagctcgtc ctgggcttcc
     9541 ttgacccgct tcaggacttc cttggccgcc ttggagcgta cgtccagctc tatgccgtag
     9601 gacttcaggt cctcggaccg ctggtccacg taggccgcca ggtcctcccg gatacggtcc
     9661 gtgaatttct tgaccttttc cagggaccgg ttgtcccggt ccaggaagcg gtatacggcc
     9721 aggacgatgg ccacgatggc gagggttatg aggttgcccg ggtcgaacag catgcggcgc
     9781 tccctgctgg gggtgagggt tcgggcgagc ccggtccgca tgagccgccg ctcggcgcgg
     9841 accgtccgac cgttcaggcc gaagtatata ccatgcgggc cggcaaatga agaaccgact
     9901 taactattcc aggacttaac ctcggaggtc acggtaagcc ctggaaccat agatccgaca
     9961 ggatgaacaa gattaaaaac gatgaacagg atagaaattg gagagtcggt caatctccat
    10021 cctttcttgt taatcctgtt ccatcctgta catcttgtta gacctatggt tccaagccct
    10081 gccctgctgg tcggtcgact tatctaggcg ctgatttcgc ggctgcggcg tgcggcggcg
    10141 tccacggcgt ccatgacgat ggaccggaag gcccggcgct ccatgacgtg gatccccgcg
    10201 atcgtggtgc ccgcggggga ggcgacgatg tccttgagct ccccggggtg catgcccgat
    10261 tccaggatga tccgggccgc cccctggagg gtctgggcgg cgaattcgta ggccgcctcc
    10321 cggctcatcc cgttcaggac cgccccgtcc gccagggact cgatgaagag gaagacgaag
    10381 gccggcccgg acccggagag ggcggtcacg gtatccagcc actcctcctc caccagacgc
    10441 gcccggccgg cccggcggaa gatcccgagg atctcctcca cctcctcggg gctcaccccc
    10501 tcgtccgcgc agaccgccgt catccccagg cccacggaca gggctatgtt gggcatgatg
    10561 cggacgatcc ggggaggggt ggcgaagaaa ctgcgcagcc gggcgatgga gaccccggcc
    10621 gccatggaaa tcaggacggt gtccggggac atccccgggg cggcctgggt cagcacggag
    10681 gcgacggcct tgggcttgac cgccaggagg acccggtcgg cgttccgggc caggtcctcc
    10741 ggggactcct ggacgagaag gccgtgccgc tccgcgaggc gctccgcccg aggccgggtg
    10801 cggttccaga tgaggccccg gccctccccg atggcaggcc ccagggaatc caggagtatc
    10861 tcgacgatgc gaccggctcc catgaagccg aaggtccggg aggaatccga agtgtccatg
    10921 cccgatagga taaagaaatc cgtcccgatg gggaagtcga ggggattcat atatcagaag
    10981 cggcttgcga accccggcgc gtgcgcatag aatgtaaggg ccggggctgc ttgttcgaac
    11041 gatagacgag gtcccgcggg gaagtgcgac gtgtccgacg aaatagatcc cgaaatagcg
    11101 gcgctgctgg gtacggatac cggccccaag ggcccggcgg aacggaagtc cgccgccgac
    11161 actcccgatt tcgactccct gttcgggggc gccggagtcg tcatggagga acggaacaag
    11221 gcggaattcg acgtcgacct gacccgggcc cggatagccc cggtcaccaa ggatcaggag
    11281 tccgtgccga acgacttctt ctccgacccg gacttctacc ggaaggccct ggccgcggaa
    11341 ggggaggaat ctcagaagct ccacgccctc ctgtccaagt acctccagac caaggacccc
    11401 aaggatcggg gggtctaccg ccagcagttg atcccggcct actggaacct cctgcaccgg
    11461 gtcgccctca agtgcgcgtc cccgggcttc ccgattgcca agcaggtcct gctccggtac
    11521 ggcgtcctgc tacccaccct cctttcgccg gaacagcagg acatgatcca gcgggtggtc
    11581 cacaagaagg agctggacga gcccttctat tatatggacg agtggctccg ggcggtcgcc
    11641 ctgggtcatg tggggcagag cgccacggac gaggtgaagt cccggtccgg ggacgaccgc
    11701 gcccggttca acgccatcct ccagaaggtg cagggcaaga aggagactgc ggagagcctg
    11761 atgaaggcca aggccgagac ccggcgaacc ctggaaggcc tcctgcggga aaagctggac
    11821 ctcctggccc agcacgactc cgtgccgggc ttctaccacg tcccggctcc ctataccgaa
    11881 gcccagaaga aggtcatgtc ggagttccag gaggtgttcc gggccatgct ccaggcggac
    11941 aaggaactcg ggtcctccat cgaggccttc aaggacgcca cggcggagct ggaatcggtc
    12001 cgccagaaga tggacggcct ggaggaggac gtgaaggcga acttccaggc cctggcccag
    12061 gagttcgaga cggcccgcca gatgaccaag ctctgcgtcg ggcgccaggg aaatcatttc
    12121 ccggtcctga cccgggagta cttccacggg ggctcccggg acgtcggctc ccgggaaaac
    12181 gtcgcccggc tcctggcctg gatcgagggg atcgataccc aggctttctg ccggccctac
    12241 aagaacaccc tgaaccgtat cgtgccctac gtggtgatcc tgcctaccta cggggatttc
    12301 gggatctgct gggagccctt cgacaagttc aaccgcgcca ccagccgggg ccgcctggcg
    12361 gtgcccctgt atcccaagag cctccagctg gcggtggtga ccgccgtggc ggacctgagg
    12421 tggcaggtgg ccaaggagaa ggcctcgtac tactggatgg aggagggcct cacgggcaac
    12481 tactaccagt ggttcacgtc caagaagatg aagggcgacg tcaaggagtt cttcatccag
    12541 gactacatcc tgtggatcac gaaggagagc gaggggaccc agaagctgga caaggaggtc
    12601 cgcggggtct tctggcggta catgcccttc gcccaggaga tcaaggacaa gctgaagacc
    12661 cggagctacg tctaccagga gctctaccag aaggacatca accgctcgat gtccgacggg
    12721 tactgatccc cgtagccagg tacacccctc cgaccaccac gactccgccc gccgcctgga
    12781 ggggggatag gcgttccccc aggagggcga aggacgccgc cacggagacc acgggtatca
    12841 ggttgatgtg gacgttgacg accccgggtc ccaggacctc caggcccgtg acgtagaaga
    12901 ccgtggccag ggcggacccg aagatcccca ggaagaggac gttcaggcct actccccaac
    12961 cgaaaccggc ccaattcacc gtctcgaaca gggcgaaggg cacgaagccc gccatgccga
    13021 agaggctctg ccagaaggtg atctccagcc ggtcgtaccg ggacgccagg ggccgggtca
    13081 ggaagccgta gccgacccac gcgaaggcgg ccaggagcat gtagaggtag ccggcggggg
    13141 atcggctgaa ccggaaggat tccgccacca cgaggccgat cccggcggcg ctcaggatcg
    13201 cgcccgcggc cttcgaggcc gagaggcgcg tgcggaggaa ggcgctctcc gccaggaggg
    13261 tgaacacggg gatggtcgcg gtcatgaggc tggcctcgga ggcggagatc cgcaggatgc
    13321 cgttgttctc gaagaagaag taggccgtca cccccaatag gccgctggcg gccatgcggg
    13381 ccgcgtccgc ccggtccaga cgcggagggc ggcgccgtgc ggccaggagc ccggccaaca
    13441 ggatcccggc cacggcgaag cgggccaggc ccaggctcat ggggggtagg gcgcccaggg
    13501 ctacttttat gctgaggaag gtgaaacccc agatgaagga ggccgtcagg gaagaaagga
    13561 cggccaggcg gcgggagacg gccatggtta ccgcgcgggg cggccttccc cggccttggc
    13621 ctcgtcccag agctcgtcca tccgggccat gttgtccgcg gacaggggaa gcccctcccg
    13681 ggccatgccc ttctccacgt ggcggaacct acgggagaag cgctccaccg ccccgtgcag
    13741 ggccaccgag ggatccaccc ccagggcccg ggaaaggttc accgccgaaa agaggatgtc
    13801 cccgatctcg gcctcgaggg ccggtcggtc cccggtttcc cgggcctcct cggtctcggc
    13861 gatctcctcc cggagcttgg cgaagacgtc ccgcacgtcg gtccagtcga acccggcctt
    13921 ggcggccttc ttctgtagct tgtaggcccg ctccaggggc ggcagggccc gggagacctc
    13981 gtccaggacc gagtccttgg gtctccgtcc ctccacggtc tccttgatct cgttccactg
    14041 ccggatcacc gcgtccgggg tggaggcctc gctgtccccg aagacgtggg ggtggcggcg
    14101 gatgagcttg tccgatatgg agtccaggca gtcggcgacc gtgaagagtc cccgctcctc
    14161 gtacatgtgg gcgatcatgg tcgtaaggag gtagaggtcc ccggcctcct cccggacatg
    14221 ggcggggtcc ttctcgtcga cggcctccac gagctcgtag gcttcctcca ggaggttccc
    14281 ccgcaggctt tccggggtct gctcccggtc ccagggacag ccctccgggg agcggagggt
    14341 gcgtacgatt ccatggaggc gttcgaaggc gtcggggctg ctcatgggcc cactctgccc
    14401 caaagcgggc cggggaggca agtcctcccc gtctgcacga gtccggctgc gccggactcg
    14461 tgcagacgaa gcgggcccta gaagatgtcc aggcccttct cccggccctc cggcaccagc
    14521 ccgacgaggg cccggatgtc gccggcgccc aggaagaacc ccgtctccag gcgccccggg
    14581 gcggccgcga agtatccgta gaggcggtcc aggccctccg aggggagctt gcccttgtcc
    14641 ttggagaagg cggccgcgag gccgaagatc ttcttcaggg agaggtagta ggcaccccgg
    14701 atttccttgg ggaggtcggc cgcgaaggcc ttgtaggacg cctccccgcc caggggccgg
    14761 gccgccccgt cccgccggac cgcggcgggg agcttggccg gatcccccag gaccaggagg
    14821 taccggtcct tgacgtaccc ggcgtggaag gtcagcttgt ccaggaggac gtccaccacg
    14881 gcccgggcac ccggatcgga cccgagcccc tccccgccga ggtcgatccg gatcgagtcg
    14941 taggggatcc cctccacgct gccctccgaa acctccaggt ccagggtaag ccccgaggaa
    15001 cccaggagct ctcggaaggc ctcgccgaag aggcccgccc cgccgagatt cctgaggacg
    15061 gagcggtagg cctcccgatc ccggagggtg ccggccccgg cggcctcgaa ggccagggac
    15121 cgttccagga gctcggagat ctcccccggg ctctgggcgc gttcggcctt ctggaggaag
    15181 tccgggtcta cggtcaggtc gaaggagaag gcgctgtccg cccccagggc cgcgtaggag
    15241 gattccagga gctcgaagta ctccgtcccc aggagctttt ccatgcccag ggccttgacg
    15301 tacagctccg ccaagtccgc cagggcccgg gggtcgaagg aggcggcccc gcccatgagg
    15361 gagtcggcct ccaggtactt caggaagggg ataccccggg cgggtcccgc cgccgcggcc
    15421 agtctgccga gggcccggtc gggatgggga acggcgccgg cccggaagtg gaatccccgt
    15481 ccgtccgcgc ccacggcgaa gtccagggtc cggagattct ccgcggcctt cttaagcagt
    15541 tcctccgggt ccgccgcgtc gcccaggagg ccggccatcg gaccggagcc gcccgtctct
    15601 ccctcgtatt cattccagtc gaagttgcgc tcgttgtagt tgtacggagc gtcgggatcc
    15661 tcgtcgagct cgagcccggg ctcgccgtcc tcgtccccgt tcccgaaggc gtccttcagg
    15721 gcggagtccc accggtcctg gaagtccgcg cgaagggctt ccacgttgat ccaggccttc
    15781 acggagccgg cggggtaggc cgagagccgg gacaggtccg cggtcttttt cgggagcgcg
    15841 gacggagccg gtcccttcag ccccaggacc gcgtagccct ccacgaaggc ggcttccccc
    15901 tcgaacccgg tcagggctgc ccgcaagcgc tcgaaggctc ccttttcctt cttcaggggc
    15961 agccacagga ccgcttgcgg cgagccttcc gccccgttcc cgtccgggag gaccgaaagg
    16021 accaggggcc gcccgaaatc caggacctcc gaggcttccc ggaagccctc cttgccgccc
    16081 gcctcctccc ggaggagttc cgccagggtg cgtcccttgg tgaaccggtc caggccggcc
    16141 tcccggacga agtcctcggc ggaacggaag agcgaggcgg gggactccac caccagggcc
    16201 gcgtaggcgt ccgaggggac caggcgggag gcgtccccgc cggaggatac gcaggaggcc
    16261 aggacgagga cggcgacagc cgccgggatc aggattcttc tcaagtcatt cccctttcaa
    16321 ccggaaattc cggaggctgt cggccacccg ggccgcgttg gactggttgt tttccgccag
    16381 ggacgaggtc ctggagtagg cggacttgag gactcccccg ttggaccgca ccgcctcgat
    16441 ggagacctgg atgtcctcgg agtatcggtt gaggtccttc atctcgcccg cgatgagctc
    16501 cgccaggtcg gcgaccgagg ccgctccccg gtccatctcc ccggaggtgg acttcatcct
    16561 gtccagggag gccaggatct ggttggatcc cgcgctctgt tcctccatgg ccgccttgac
    16621 ctcctcctcc agccgggtga tggtccggat cagggactgg atctccccga agttctcccc
    16681 ggccctgcgg gaggagtccg cgatggagcg gatggcggac gcgacctccc cgagcatggc
    16741 ctccacggcc ttggactgct ccgcggcgtt ttccgcaagc ttgcggatct cgtcggccac
    16801 caccgcgaag ccctttccgg cctcgcccgc gtgggccgcc tcgatggcgg cgttcatgga
    16861 gaggaggttg gtctgggaag cgatgttcgc gatgaccgtg ttggtctcca gcagggcttc
    16921 cgacctcgaa aggatgtgct gtgccttctc tatgacctcg gacaggagtt cctctccccg
    16981 ggcggaggac tcgttgagcc ggccgtactg ctcgctggcc ttgaccacgc tcccggtgac
    17041 ggcgcggatg ttggccaaga tctcctcgat ggcggccacg gactggagga tcatctcgga
    17101 ttcgtcccgg gaccgggctt ccaggctccg ggcgctctcc tggatccgtt ccaccgaggc
    17161 cgtcacggcc ttgatctccc ggtctatgtc cgtgccccgg cccaccacgg tctccacgga
    17221 ccggtcggtt ccctccaggg cggtccgggt gcgttccatc tccgccgcca ggtccccggc
    17281 gttggcttcc agggtgccca gggcggactt gagatccccc aggactccgt tcagggtgtc
    17341 ggtcaggcgg ttgaagcccg cggccaggcg ccccgtctcg tccttccccc gtacgttggc
    17401 cgcgggacgg aggtccccgg aggcgattac gtcgaaggtc cccgtcaggc tcaccagggg
    17461 gcggctgatg cgccgggaga ccgcgacgat cagggcggtc agcagggcca ggacggccag
    17521 ttcggcgacg gtcagctcca ggtagatccg cctcgggatc tccaggatct tctccttggg
    17581 cagggatacg atgaagatcc aggggtccag gttctcgtgg aggcggacgc tctccatcat
    17641 ctggaggacc gcccggccgt cgatctcggc ctccaccgcg gtttcccggg ccgtatccgg
    17701 ccgggtcagc tcggaggcgg cggatacggt gtccgccgag tgggaatccg acaggtagga
    17761 accctgcccc tcggcaaggg tgtccgccag gatcaaaccc gcgtagttcg cgatggtgga
    17821 cgccccgccg ctccgctcgt ccacgtccgc cagaatcccc tccaggaagt ccagggagaa
    17881 atcgacgccc gccaccccca ggatcccgcc gttcccgtcc tccagcctgc gcgccagggt
    17941 gatcatgcgc acggtgcggc cgtcctccac ctcctcctcg tagggatcgc tgagccagaa
    18001 cccggagcgg gcgagagggc ccgtatagta ttcctccccg taggcctcct cggcccaggc
    18061 ctcctcggag agcgtcgcgg acccggcctc gtcccggtag gcccagacgg cgaaggcccc
    18121 cgtatcctcc gggcccaggg ttcccgcgaa ccgggagtcc cggccgtccc aggcgttggg
    18181 ttcgaagagg atccagaggg cgaaggccga gtcatgggtc cgcagggtgt ccagcaagac
    18241 cgcgggcagg atggctcgat cgacgcgccc ctcccgcttc agggtctccg cgagccgggc
    18301 gctgtcctcg aacatgttca gggattcccg gaaccggcgc tccgccaggg aggccccctc
    18361 ccgggcatac tgggcggccg cccggtacgc gtagtcggtg atcgtcccgg aggtgtcgta
    18421 ccccaggaag gccacggccc cggtgaacag gaggaaggtg ccgggaatga ggaggaggag
    18481 catctttgcc tggatggatc tcatcgatga aagccgcctt gccgtgcgag ggatacccct
    18541 aaggatatga cgggaatcgg ggaaatcaat ggaaacgggg tgtcccggcg cggattccgc
    18601 gcgagacacc ccgtactagg agtctgtcgg acgggccctc aggtttccgc gatcaggagg
    18661 gtcttggcgt ccagcttgat cgagaggtcc tcgggattca tccgctcgtg gtccttgacg
    18721 accaggaagt ggatgtactc ggccttcggc tcgaggacga tgaggacgga aatgctgtcc
    18781 aggaactccc ggagcatgac cggcacgttc cgcttgtccg tgaggggctc ctccccgacc
    18841 atggtgcagg agtaccagac ctccaggttc atccgggccg cgaaatcctt gaccttggcc
    18901 aggtggtccg gcgcggaccg ggtgaagtcg aatccgtcga tgatgaccga atccgccttg
    18961 aagccgccgt ccacgatcat ggcccccagg gagcgtagga tctgctccgt ggagatgcct
    19021 tcctggttga agttcatgat gacccggttc ttcaccaggt cgtcgtggat gtcctccacg
    19081 ttctccaggt tgcgcttccg ggtgagctcg gtaaatatat tttcatacca ggagatgacg
    19141 tagctggtat gggtggtgaa ggagacgtgt acgacctttt tcccctggag gagcttgtcc
    19201 agggccagct gcacgagcac gctggtcttg ccgataccct tccggctggc gatcacgccg
    19261 atgtttccgc ttttcagtcc gccctctatg ccccgttcga ggatgcggac gggactcttc
    19321 ttgataagct cctctttaag catgggaact ccttggtttg ttttcggcgg gccggatcga
    19381 ggccgatccg gcccgtgctt taagtatagg tccagtcccg ccgccgggca acgggaaaac
    19441 cgcggcctag cggccctgtt ccttcctccg cttctccaga tagtccttgc ggagctgctc
    19501 ggagacgctg gtcgggacct tggcgtactt gaggaactcc atggagaact ccgccttgcc
    19561 ctgggtcatg gaccggagca ccgtggaata gccgaacatc tccgccaggg gaacttcggc
    19621 ctccacgcgg cagaaggaag cctcctccgt gctggcgtgg atgacccccc gcctctggtt
    19681 gacggaagcg aagatgttgc cctggaactc cgtcgggccc tcggccacga ccttcatgat
    19741 cggctccagg atgacgggct tggccttctc gtaggcctcc cggaaggccc cgatggcggc
    19801 ctgctggaag gcgatgtccg aggagtcgac cgggtggctc tggccgtcgt tgacgacgca
    19861 gcgcacgttc acgatggg
//
LOCUS       DAOTEI010000049        19466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1841_length_19466_cov_5.267683, whole genome shotgun sequence.
ACCESSION   DAOTEI010000049 DAOTEI010000000
VERSION     DAOTEI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 19466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19466
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_19466_cov_5.267683"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="P5313_06660"
     CDS             <1..237
                     /locus_tag="P5313_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80077.1"
                     /translation="KRSIETLFDIIPVGLVALSPENEVVRSNPAFRVLMERWSTRLVM
                     SNHRKGSRTVVTILSASYGVSVPEKLFNPTQFYK"
     gene            249..1625
                     /locus_tag="P5313_06665"
     CDS             249..1625
                     /locus_tag="P5313_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80078.1"
                     /translation="MDKHRSMRSGGAIRAVLAAVLLAGTGVARAQDPASGPGDGNLVP
                     EPFAVYGYARVTAGGYGFAVPPGTTAGADFRVRLKADWNPSGSLAFRLEAEYDAAYGG
                     ANEYARLGHYGIPTYLASVLAALGGPASSSGDLYPYEDLHGSLSIDHAYGRANFGPLD
                     LAFGKMPLAWGTAYVFNPTSRTGPAGGLDPGSEETPGVLALAPSLALGGNSSVQAYLA
                     FQERSLRGDSTLEEGSPDNLPFGVKVQAVIGTFDLSFSFLREVLWEETLAGTAAFRRR
                     AWLGLDFAGALGPVGVYGEAVLGLPASVLSGTGPADFDAARDLEASGGIDWTVPGIRV
                     EVRGEYHYRGSGPVRPEEYDPSVLLSGASSVLGRHYLFLYAARTFSDYWTASLAGLAN
                     AGDGSWVLLPRFIWDAKENLELEAGAVLPFGPGGSEFDGRRDLAAYGLGTVDLTAPTA
                     FVSLKLSF"
     gene            1763..2881
                     /locus_tag="P5313_06670"
     CDS             1763..2881
                     /locus_tag="P5313_06670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY80079.1"
                     /translation="MTQPQPDFELRRSGGSFRDPAREEEFRRFELDAASPFLRKLLPL
                     LGTLFAAFVVPDYWILGPGPSFFLTAVARGVSFGLLIFASSQMTPEKPRVRRERLLVG
                     ITLLGITSFGVALFTYRKANFHLQAVSVVLMIAAIYLIPNRFRVSLLLSLLLAGMGLA
                     YLLLLAPPELLASERAAYIADFLLMALLSALLGRTFSATRRREYARTRELEWISRTDL
                     LTKVGNRRDFEDRLESSLARRRRHGEETALLMLDIDKFKSVNDQFGHHVGDAVLREIA
                     ERLESSLRTEDSLARWGGEEFVLLLPRADLKSAEEFGGRIRSRISGTPFGCAGTITAS
                     IGITMLSETDTVESAVARVDRALYRAKDNGRDRIECEG"
     gene            complement(2892..3320)
                     /locus_tag="P5313_06675"
     CDS             complement(2892..3320)
                     /locus_tag="P5313_06675"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HRY80080.1"
                     /translation="MNRIGIAAALTLLAAASAEAQEAVVRLTVSGVKPAGGTVYTAVF
                     DSADGYRKNVPLRSLTLQPASSVVEATVTLPPGEYVFSLYQDLNSNGKLDTNFIGIPR
                     EPVGISNYDGKGPPGGFDRQKVRVPSGGMSLAIRLISINP"
     gene            complement(3317..5179)
                     /locus_tag="P5313_06680"
     CDS             complement(3317..5179)
                     /locus_tag="P5313_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRY80081.1"
                     /translation="MPTDSASPSPPAGPEDRPSVARGSGFVEDDFLYSAHPDPSWARR
                     SWVSLDGTWKVRRGGKVREIRVPFPIGSELGGAEFPDRGNFRFSRSFELSPEAAGGSV
                     ILRIGACDYETGVEINGRLVERHVGGYASFSVDLSGLVRPGGNELVLRVRDSHSPFQV
                     RGKQTFLPGPFMVWYAGISGPWQPLWLETTGTPYLRRPRVRADFDAGVLRIEARAAGA
                     GPGSLRLSVVLESPGGEASRFEADAEGGSGDSGGVFRIDIPFEAVPGPRWSPETPELH
                     KVRYTLYEGDRVSDTVDSYLGLRRIEARDGRILLNGKPIALRMALVQGYYPGGWYAPE
                     SADRYRDDILALKGLGFNGARIHQKIESPYFHYLADRLGLLTTFEMPSFYLPSRKAFR
                     AFESELREVVERDAAHPSGIAWVLFNETWGIWGIYGRRSRTRAFVRSMIDLVRNLDPG
                     RPVIDNSGWEHMETDITDIHHYLETAELARSGYSALRERDPRRLHSVSVPRVIFFYIF
                     NRIGRETRSVFLDEATRKACEGTPWLVSEYGGFGWYASADPGSIPDRIEAYTRDIAES
                     GLFQGYCLTQLYDVGTETNGILDFGRNPKVPVQKMRDVNQAPFRAGDRGKDGTT"
     gene            complement(5184..5420)
                     /locus_tag="P5313_06685"
     CDS             complement(5184..5420)
                     /locus_tag="P5313_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80082.1"
                     /translation="MNSSLLEDILAVQREERTGRILYGCLAGVVKDERNARTRGLTFA
                     LRNGPLPAIRGIVTGIAATFNFRLPVRALFGIEV"
     gene            5825..6850
                     /locus_tag="P5313_06690"
     CDS             5825..6850
                     /locus_tag="P5313_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY80083.1"
                     /translation="MPVITVENLSKTFRTRVRSEGLGASLRALIRPEWRTVQAVREVS
                     FSVEEGEVVAFLGPNGAGKSTTIKMLTGILTPDSGGARVLGLEPSRDRTRLAMGIGAV
                     FGQKSQLWFHLPPSDSFRLLGAIYEIPAPELARRRAELVERFGIGPYMDVPVRKLSLG
                     ERIRCEIAASLLHAPRVLFLDEPTIGLDVVVKREIRALLADLRAREGVTIFLTSHDIG
                     DIEKICKRAIIIHHGAVVVDESMKTLKHRALARKYIGVKYAAPADLELPGLAPVKRTE
                     DAASFVVDTRKHNLQEVIRALVDRGEVLDLTVEDEPLEDIIAEVFRGRSEEEARGAAR
                     AGGEAES"
     gene            6847..7683
                     /locus_tag="P5313_06695"
     CDS             6847..7683
                     /locus_tag="P5313_06695"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRY80084.1"
                     /translation="MIAHSLKGPGRTGKPSAALRKYGTAGLVSLQSRMAYPAAFAGSL
                     LTYGLFVFVFSRIWAALYSGRAEIAGYDRTMMVWYFIVAEIPAFGFGRFYYSLAEDMK
                     SGQVAYLLTRPYGFTAYHFSQALGPALADCLVLALEGILLGLLLGGPFPLTTLTQGAA
                     LVLSLLLAGCLQFFLQFAIAMTAFWIEENAAFFWIYQKLALIAGTFLPLEFLPGAASA
                     AARWTPFPYLSWAPARIAVAWDSREALLLLAAQAAWTAAAALLCRAVFAAGRGKITAN
                     GG"
     gene            7685..8494
                     /locus_tag="P5313_06700"
     CDS             7685..8494
                     /locus_tag="P5313_06700"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRY80085.1"
                     /translation="MNRPRTGRPAAYLRFLAAAFTANLQAVVEYRISFLIQVFGMMLN
                     NAAFAAFWAVLIARTGSVGGYGFTDVMVIWALTSTSFGIAHVLWGNVRSLGSIIQRGE
                     LDVYLLQPKDPFVNVLASRTIVSAWGDLAYGYLVLAFLPGTGPERLALFTLLALGGAV
                     IFAAVFSAAESLTFFLGNAGPLSQAVTEFLISFSLYPETIFDSGMRWIFYTILPSGFI
                     AFVPLAVYRSLDWPLVPVLLGVAAVYAGGAYGLFRAGLRRYESGNQMGTRL"
     assembly_gap    8533..8632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9443..11521
                     /locus_tag="P5313_06705"
     CDS             9443..11521
                     /locus_tag="P5313_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY80086.1"
                     /translation="MNRIVDHPILSAPEGRTSVTFSFDGAALSGFEGEAVSSALFAAG
                     VQTFSVHPRGDAPQGIFCANGQCSQCTVLIDGVARKACITPLAEGMDVRTLHGFPELP
                     ARDAPRAAVSGETLSTDVLVIGGGPSGLAAAAELARLGLSVILADDKVSLGGKLVLQT
                     HKFFGSEEDCYAGTRGIEIAKRLEEDIRSLPGVTVLENSPVAGLYKDRKAGVWRNYES
                     YVLVDFKALVVAAGAREKSVLFPGNDLPGVYGAGAFQTLVNRDLVRAARRVLIIGSGN
                     VGLIGAYHALQAGIEVAGIVEILPKVNGYKVHADKIVRMGVPVYLNTTVVSVEGNGKV
                     ERATVAEVDKDWRPILSTARTYEVDTVLVAAGLSPCDEFYRQAVDFGFLAVTAGDAEE
                     IAEASSAMFGGRIAALSLAKLLGKKVDIDESWLAKREVLKSRPGDNLCRDEEIPTEFW
                     KPVFFCTEEIPCNPCTTVCPTHSIQLKERKGSIMDLPYFEGSSCKGCTVCVAACPGLA
                     MSLMRRVDEGWAEVVLPFEFKADFEPGARLPVLDQEGAFLEDAELLKKVFNRKYRTWI
                     LTLKVSRANAPRAIGIRVQSEAATRPLPAPRFEHLPDDAVVCRCERITVGEIVRFIRE
                     NAVTDVNQLKTLRVGMGACGSKTCSVLLPQVFRKAGIDPARTTAGTLRPLALEIPMKD
                     LINEAAGRPL"
     gene            11518..12666
                     /locus_tag="P5313_06710"
     CDS             11518..12666
                     /locus_tag="P5313_06710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY80087.1"
                     /translation="MKTCDVAILGAGSIGVPLAYYCAKKGLSVLVLDKEPSWGRGQNR
                     AAIGGIRATHSDPAKIKIGLLSLDIARNMKEEHGLNIEWHQGGYMFVAYDEERERSFK
                     DLLVVQKAAGLDIDWISPDRVLELSPGLNPEGLRGGTYSPGDGFASPLMTSTAFHKLA
                     LDAGAEFRFGETVTGFGIEGRRIRTVRTDAGEYSAGTFVNAAGAEAAGLGNLAGLELP
                     VHPDLHEGGVTEPVRRFMEPMVVDMRGDDRSGNYYFYQAGTGQVIFCITPKPQIWGTD
                     TDNTSAFLPHCVSRMVSLYPRLRNLKVRRTWRGCYPMTPDGLPVVGYPREPENLLLAV
                     GMCGQGFMLGPGLGRILADTLTGDGARYGQVFEELSLYRKFEGMELLK"
     gene            13017..13544
                     /locus_tag="P5313_06715"
     CDS             13017..13544
                     /locus_tag="P5313_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80088.1"
                     /translation="MQFPDADWERLCGLVAEAFRMDGSERARLAGSRAARITGALPYL
                     AGCRNPERTALAHLAAFVLACRGGTRKVFDHGPADDAEPLARLEPIARFPGGDPAVIR
                     KGMALLGLLMLGGYERDREKDTAGGEYNPLNSGAWKAGEVRARLQAEAASAKVPELDA
                     IISTDEASVRGYWDG"
     gene            13537..14274
                     /locus_tag="P5313_06720"
     CDS             13537..14274
                     /locus_tag="P5313_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY80089.1"
                     /translation="MAETKTAINPLARIGRFTALVFVAGSLLMFVVWMSRGWGFVKTI
                     THPYIGIQLLVALLFYISSRSPKLYIIQPILLLIQAPINFLDSHDSFYGLGFFVMAVL
                     LLFKIGFFERGRIPKVIGLLVYLYGWELFAAIRAGRHTGLSLTPVFFVTVFLIFLYIL
                     YKEQVIVYLKQPKPLLDLQAKGLADTEAAYILALAKGLNVKEIAGKFEVSESTVRNTL
                     ARAYKKLGVQDKAELSTFLAEYTLPEG"
     gene            14354..15502
                     /locus_tag="P5313_06725"
     CDS             14354..15502
                     /locus_tag="P5313_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY80090.1"
                     /translation="MAFFEPFRLERFFARWEFSAPHLLCCSDCESVPVSDLAALDPGL
                     PERLSSLRLGYTETRGAPSLREAIAGEYPGAAPEEILVHAGAEEAILNLCLALLSKGD
                     RVVVNTPCYQSLAEIPRALGCEVLPWEWRPAGGRWALDPGDLDRILRSGPDPKLVVLN
                     APHNPTGGLPTKAEFDAIVAAARRVGAVVLMDEVYRRLEHDPARRLPSIRDTYENGVA
                     LDVLSKHAGLAGLRIGWLATRRADILDAVAVVKDYNSICSSGPSEVIAESAVRNLSAL
                     VDRNRRIVADNLALLRRFFAARPDFAEWTEPEGSSVGFPRLAFPGDAEVLAERLVRET
                     GVLILPGSYYGCDARHFRVGYGRISMPESLARLEKWLDEGGPAAAALS"
     gene            complement(15513..16247)
                     /locus_tag="P5313_06730"
     CDS             complement(15513..16247)
                     /locus_tag="P5313_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80091.1"
                     /translation="MVLSPEGPSALRAAERSGINLYSERSLHDQVKRLLAGPGDRIEA
                     AVGGRVVDLLRSDGSVVEVQTRHLYALLPKVRALAGAGIRVRVVHPVPASLEIRRRDP
                     DSDRIVSTRRSPKRGDLWSVFDELVKAPDLISLPGVAVEVLLVRASEVRVRDGTGSWR
                     KRGDRTEDRFLEGIVGAASWESPEDWLALIPRDLPEPWSSETLGRSLGIAPARARKVL
                     YSLARAGFLREEGRAGRRKLYRNADS"
     gene            complement(16238..16762)
                     /locus_tag="P5313_06735"
     CDS             complement(16238..16762)
                     /locus_tag="P5313_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRY80092.1"
                     /translation="MDLDLSPGSRILVDSSALVYLVEDVPRRGPAVSDFLDRVRSAGG
                     RPFASVLTLTELLERPLSRGDRELALRYRRFLADSSLLVLVPVDAEVAERAARILGEG
                     TFDPRSRKLPGLRPGDPGSSRFGVSFADAVHLATALVHRAEAILTNDEAWRDVPEAPP
                     VYLVDEIAAELEWS"
     gene            16944..17231
                     /locus_tag="P5313_06740"
     CDS             16944..17231
                     /locus_tag="P5313_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80093.1"
                     /translation="MKSLMPLVLLAIILALLVVTNPTKQDFAAWYGKQAAASAPSGTL
                     GKALGGIGKGMASVAASAFQRQSYGVLSVFSLAKGGTAYVGLGKVVFLKVR"
     gene            17363..18670
                     /gene="ugpB"
                     /locus_tag="P5313_06745"
     CDS             17363..18670
                     /gene="ugpB"
                     /locus_tag="P5313_06745"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HRY80094.1"
                     /translation="MKRFSLLMLALLLCASTIFAQIQIDWWHAMTGKNGQMVEELAKR
                     FNDSQTAYKVVPVYKGSYSDTMNAGIAAFRAKQAPHIIQVYEVGTATMMNAGGAVYPV
                     YKLMKDSGEKFDPKAYIPTITGYYSTSKGEMLSLPFNSSTAVMYYNKDAFRKAGLDPE
                     KPPVTWPEFFEVAKKLKASGMEGGFSTNWISWIQLENFSAWHNQSFGTKNNGFDGLDT
                     QLVFNTPLTIKHFETLYELSKDKTFIYGGRENKANPLFTSGTVGMHFESIGGYGNFKN
                     TAKFDFGVARLPYYPEVKDAPQNTIIGGASLWVFNGKPKDDYKGVAKFFTFLSQPEIQ
                     AFWHQSTGYVPITVASYELTKKQGFYDANPGPEVAIKQLLNKAPTGNSRGIRFGNFNI
                     IREIEDQTWEDILAGKISVRDGLNKMVADGNAELRKFERLYKK"
     gene            18768..>19466
                     /locus_tag="P5313_06750"
     CDS             18768..>19466
                     /locus_tag="P5313_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY80095.1"
                     /translation="MAKQYEFRIKGLPYLLVLPQMVIVFIFFFWPAAQAIWQSFFLQD
                     PFGGQYIFVGLENYQALFTDEASAYAQSFWVSVFFAVSVTVLAMSLSLFLAVQANKKI
                     RAASFYKTMMIWPYAVATLIAGVLWLFMFNPNVGVVAWFLKHRLGYEWQHLLNTGQAL
                     TLLILAASWRQMSYNFVFFLAGLQSVPNDFIEAAAIDGAGPARRFWKIIFPILSPTTF
                     YLLVMNLVYGFFETF"
BASE COUNT         3155 a         6747 c         6239 g         3225 t
ORIGIN      
        1 aagcgctcca tcgaaacgtt gtttgatatc attccggtgg ggctggtggc gttgtcgccg
       61 gagaacgagg tggtgcgcag taacccagcc tttcgtgtgc tgatggagcg gtggtcgacc
      121 cggctggtca tgtccaacca ccgcaagggc tcccggaccg ttgtgaccat cctctcggca
      181 tcctacgggg tctcggtgcc ggaaaagctg ttcaacccga cccagttcta caaatgaagg
      241 aggtcatcgt ggacaagcac cgaagcatgc gatcgggcgg ggcgatccgg gccgtcctgg
      301 ccgcggtcct cctggcgggg accggcgtcg cgcgggccca ggacccggcc tccggcccgg
      361 gggacggcaa cctcgtcccg gagcccttcg cggtatacgg gtacgcccgg gtgacggccg
      421 gaggctacgg cttcgcggtt cccccgggta cgacagccgg cgccgacttc cgggtgcgcc
      481 tcaaggcgga ttggaacccc tccgggagcc tggccttccg cctggaggcg gagtacgacg
      541 ccgcctacgg cggagcgaac gaatacgccc ggctcggcca ctacgggatc ccgacctacc
      601 tggcgtccgt cctggccgcc ctgggaggcc cggcgtcctc gtccggggac ctctacccct
      661 acgaggatct ccacggctcg ctttcgatcg accacgccta cggccgtgcg aacttcggcc
      721 ccctcgatct ggcgttcggc aagatgcccc tggcctgggg taccgcctac gtcttcaacc
      781 ccacctcccg gaccggcccc gcgggaggcc tcgacccggg ttccgaggaa accccggggg
      841 tgctggccct ggccccgagc ctggccctgg gcgggaactc ctcggtgcag gcctatctgg
      901 ccttccagga gcggagcctc cggggcgact ccaccctgga ggagggaagc ccggacaacc
      961 tgcccttcgg cgtgaaggtc caggctgtga tcggaacctt cgacctgtcc ttctcgttcc
     1021 tgcgggaggt gctctgggag gagaccctgg cgggaaccgc ggccttccgg cgccgggcct
     1081 ggctgggcct ggacttcgcg ggggccctgg gccccgtcgg cgtctacggc gaggccgtcc
     1141 tgggccttcc ggcctcggtg ctctccggca cggggcccgc ggacttcgat gccgcccgag
     1201 acctggaagc ctccggaggc atcgattgga cggtgcccgg aatccgggtc gaggtccggg
     1261 gagagtacca ctaccggggt tccggcccgg tccggcccga ggaatacgac ccttcggtcc
     1321 tcctgtccgg ggcttcctcc gtgctcgggc ggcactacct cttcctgtac gccgcccgga
     1381 ccttctcgga ctattggacg gcgagcctgg ccggcctggc caacgccggg gacggatcct
     1441 gggttctgct gccgcggttc atctgggatg cgaaggagaa cctggaactc gaggcggggg
     1501 ccgtcctgcc cttcggaccg ggaggaagcg agttcgacgg ccgtcgggac cttgcggcct
     1561 acggcctggg gaccgtggac ctgaccgcac cgacggcctt cgtctccctg aaactgagct
     1621 tctaggagcc tgtcggattc ataccccctc ccgaggcttg ccaagccgga cggcggggag
     1681 tatcgttccc ccatggaaaa actcgatgac tccgggtccc ggtaagctgg atgtcggtcg
     1741 gacggcgagg tagagtacaa ccatgacgca accccagccg gacttcgagc tccgcagatc
     1801 cggaggttcc ttccgcgacc ccgcccggga ggaggagttc cggcgcttcg aactcgacgc
     1861 ggcctcgccg ttcctccgaa agctcctgcc cctcctggga accctgttcg ccgccttcgt
     1921 cgtgccggat tactggatcc tgggccccgg accttccttc tttttgaccg cggtcgcccg
     1981 aggcgtatcc ttcggcctct tgatcttcgc gtcctcccag atgacgccgg agaagccccg
     2041 ggtccgcagg gaacgcctgc tggtcgggat cacgctcctg gggatcacct ccttcggggt
     2101 ggccctcttc acctaccgga aagcgaattt ccatctccag gccgtcagcg tcgtcctgat
     2161 gatcgcggcg atctacctca tacccaaccg gttccgggtg tcgctcctgc tttccctgct
     2221 cctggcgggc atgggcctgg cgtacctgct gctcttggcg cctcccgaac tcctcgcgtc
     2281 ggaacgggcc gcgtacatcg cggacttcct gctgatggcc ctgctgtccg cgctcctcgg
     2341 aaggactttc tcggcaaccc ggcgcaggga atacgcccgc acccgggaac tggagtggat
     2401 ctcccggacg gacctgctaa ccaaggtcgg aaaccggagg gacttcgagg accgcctgga
     2461 atcctccctg gcccggagaa ggcggcacgg cgaggagacc gccctcctca tgctggatat
     2521 cgacaagttc aagtccgtga acgatcagtt cgggcatcac gtcggagacg cggtcctccg
     2581 ggagatcgcc gaacggctgg agtcctccct tcggacggag gattccctgg cccgatgggg
     2641 aggggaggaa ttcgtcctac tcctgccccg ggccgacttg aaatcggccg aggagttcgg
     2701 cggccggatc cggtcgcgca tctccggcac ccccttcgga tgcgccggga ccatcacggc
     2761 cagcatcggc atcacgatgc tctccgaaac ggataccgtg gagagcgcgg tcgcccgggt
     2821 ggaccgggcc ctgtaccggg cgaaggacaa cggccgggac aggatcgaat gcgagggcta
     2881 ggcggccgcc gtcagggatt gatcgagatc aggcggatcg ccaacgacat ccctcccgag
     2941 ggcacccgga ccttctgcct gtcgaagccc cccggaggcc ccttgccgtc gtagttgctg
     3001 atccccacgg gctcccgggg tatgccgatg aaattcgtgt ccagctttcc gttggagttg
     3061 aggtcctggt acagggagaa gacgtactcc cccgggggca gggtgaccgt cgcttccaca
     3121 accgaggagg ctggttggag ggtcagggag cggaggggaa cgttcttccg atagccgtcc
     3181 gcggagtcga aaaccgcggt gtatacggtt cccccggcgg gcttcactcc cgagacggtc
     3241 aagcgcacga ccgcttcctg agcctccgcg gatgccgcgg ccaggagggt gagcgccgcc
     3301 gcgatcccga ttcgattcat gtcgttccgt cctttccccg atccccggca cggaagggcg
     3361 cttggttcac gtctcgcatc ttctgcacgg gcaccttggg gttccgtccg aagtccagga
     3421 ttccgttcgt ctccgtcccc acgtcgtaga gctgggtaag gcagtaaccc tggaacagcc
     3481 cgctctccgc gatgtcccgg gtgtacgcct cgatgcggtc cgggatcgag cccggatcgg
     3541 cgctggcgta ccagccgaag cctccgtact cggagaccag ccacggggtc ccttcgcagg
     3601 ccttccgggt ggcctcgtcc aggaacaccg aacgggtctc ccttccgata cggttgaata
     3661 tatagaaaaa gatcacccga ggcacggaaa ccgaatgaag ccgtcgcgga tcccgctccc
     3721 ggagcgccga atagccggac cgcgcaagct ccgcggtctc gagatagtgg tggatatccg
     3781 tgatgtccgt ttccatgtgc tcccagccgg aattgtcgat caccggccga ccgggatcca
     3841 ggttccggac caggtcgatc atggaccgca caaacgcccg ggtccgggac cggcggccgt
     3901 agatccccca gatcccccag gtttcgttga acaacaccca ggcgatcccc gagggatggg
     3961 ccgcgtcccg ctccacgacc tcgcgaagtt cggactcgaa ggcccggaag gccttccggg
     4021 agggcaggta gaaggaaggc atctcgaagg tcgtgaggag ccccaggcgg tccgccaggt
     4081 agtggaagta ggggctctcg atcttctggt ggatccgcgc cccgttgaag cccagcccct
     4141 tcagggcgag gatgtcgtcc cggtaccgat cggcggattc gggcgcgtac cagcccccgg
     4201 gatagtaccc ctgcaccagg gccatgcgca gggcgatggg ctttccgttc aggaggatcc
     4261 ggccgtcccg ggcctcgatc ctgcgcagac ccaaataact atcgacggtg tccgagactc
     4321 ggtctccctc gtacaaagta taacgaacct tgtgaagctc cggggtttcc ggcgaccacc
     4381 ggggacccgg tacggcctcg aaggggatgt cgatccggaa gaccccgccg gaatccccgg
     4441 accctccctc cgcatccgct tcgaacctcg aggcttcgcc gccgggagat tcgaggacca
     4501 cggaaaggcg gagggacccc ggccccgccc cggccgcgcg ggcctcgatc cgcaggaccc
     4561 ccgcgtcgaa gtccgcacgc acccggggcc gccgaaggta cggagttccc gtcgtttcca
     4621 accagagggg ctgccagggg ccggagattc cggcgtacca gaccatgaag ggtccgggca
     4681 aaaaggtctg cttcccccgg acctggaagg ggctgtggga atcccgcacc cgaaggacaa
     4741 gttcgttgcc ccccggccgg acaaggcccg acaggtccac ggaaaaggag gcgtatcccc
     4801 cgacatgcct ttccacgagg cggccgttga tctccacccc ggtctcgtag tcgcaggcgc
     4861 cgatccgcag gatcacggag ccgccggccg cctcggggct cagctcgaag gaccgggaaa
     4921 accggaagtt gccccggtcc gggaactccg ccccgcccag ttcgctcccg atcgggaacg
     4981 ggacccggat ctcccggacc ttcccgcccc gccggacctt ccatgtgccg tcgagggaca
     5041 cccaggagcg gcgggcccag gagggatcgg gatgggcgga gtacaggaag tcgtcctcga
     5101 cgaagccgga gccccgggcg acggagggcc gatcctccgg gccggcgggc ggcgagggcg
     5161 aagcagagtc cgtcggcatc ccgctagacc tcgatcccga acagagcccg gaccggcagg
     5221 cggaagttga aggtcgcggc gatccccgtg acgatcccgc ggatggccgg caggggaccg
     5281 ttcctcaggg caaaggtcag gccccgagtt cgggcgttcc tctcgtcctt gaccaccccg
     5341 gccaggcacc cgtacaggat ccggccggtc cgttcctcgc gctgaacggc gaggatatcc
     5401 tcgaggagtg aggagttcat gatgtattcc tccgaagcct gccgtcgcgg cggcaacaat
     5461 gcattatgga gcggtgcctt tatagccgta aagaggaatc acgccctagg tggcacgaag
     5521 attgtgataa ccctaagaac accgtggata ctgatatcca agcaaggatc aacaaattta
     5581 ttctatatat agaaacactt tacccccttg accttccgat aattccggat ttactcttcc
     5641 cggaacctcg agagggcgcc tagggttcct cgtccgcgga aacgcggacg gcaaggtccg
     5701 agcggcgccg gcgggcgctt tccgcccgca cacccacggg aaaaaagccc gaggacggag
     5761 tctcgaccac cgaatcccgc ttcccggccg ccgcggccgg gcgggagcgc accgggaggt
     5821 cgccgtgccc gtcataaccg tcgagaatct gtccaagaca ttccgcaccc gcgtgcggag
     5881 cgaggggctg ggggcatccc tccgcgccct gatccgaccc gaatggcgaa ccgtccaggc
     5941 ggtccgggag gtatccttct ccgtggagga gggggaggtc gtggccttcc tgggcccgaa
     6001 cggcgccggc aagtccacga ccatcaagat gctcacgggc atcctgacgc cggattccgg
     6061 cggagcccgg gtcctggggc tcgaaccctc cagggaccgg acccgcctgg ccatgggcat
     6121 cggggcggta ttcggccaga agagccagct ctggttccac ctgcccccgt cggattcctt
     6181 tcgcctcctg ggggcgatct acgagatccc cgccccggag ctcgcccgcc gcagggccga
     6241 gctggtggag cgcttcggga tcggccccta catggacgtc ccggtgcgca agctctccct
     6301 gggggaacgg atccgctgcg agatcgccgc cagcctcctc cacgcccccc gggtcctctt
     6361 cctcgacgag cccaccatcg gactggacgt ggtggtgaag cgggagatcc gggccctcct
     6421 cgcggacctg cgggcccggg agggggtcac gatcttcctg accagccacg acatcgggga
     6481 catcgagaag atctgcaagc gggcgatcat catccaccac ggcgcggtgg tggtggacga
     6541 atccatgaag accctcaagc accgagccct tgcccgaaag tacatcgggg tgaagtacgc
     6601 ggctcccgcg gacctggagc ttccgggcct ggccccggtg aagcggaccg aggacgcggc
     6661 cagcttcgtc gtggacaccc ggaagcacaa tctccaggag gtcatccgcg ccctggtgga
     6721 ccggggcgag gtcctggacc tcaccgtgga ggacgagccc ctggaggaca tcatcgccga
     6781 ggtgttccgc ggccggagcg aggaggaggc ccgcggcgcc gcccgggcgg gcggggaggc
     6841 cgaatcgtga tcgcgcacag cctgaaggga ccgggacgga cgggaaagcc gtcggcggcc
     6901 ctccggaaat acggcaccgc ggggctcgtc agcctgcaga gccggatggc ctatcccgcg
     6961 gccttcgcgg ggagcctcct gacctacgga ctcttcgtct tcgtgttctc ccggatctgg
     7021 gcggccctct attccggtcg cgccgaaatc gccgggtacg accggaccat gatggtctgg
     7081 tacttcatcg tcgcggagat accggccttc ggcttcggac ggttctacta ctccctggcg
     7141 gaggacatga agagcgggca ggtggcctac ctgctgaccc ggccgtatgg gttcacggcc
     7201 taccacttct cccaggccct gggtccggcc ctggcggatt gcctggtgtt ggccttggaa
     7261 gggatcctgc tgggcctcct gctgggaggt ccgtttcccc tgaccacact tacccagggt
     7321 gcggccctcg tcctttccct gctccttgcg ggctgcctcc agttcttcct gcagttcgcc
     7381 atcgcgatga ccgccttctg gatcgaggag aacgccgcct tcttctggat ctaccagaag
     7441 ctcgccctga tagccgggac cttcctgccc ctggaattcc ttccgggagc cgcctcggcc
     7501 gcggcccgat ggacaccctt cccctacctt tcctgggcgc ccgctcgcat cgccgtggct
     7561 tgggattccc gggaggccct tctcctgctg gcggcccagg cggcctggac cgccgcggcc
     7621 gcactcctgt gccgggccgt gttcgcggcg ggacgcggca agatcaccgc gaacggaggc
     7681 tgagatgaac cgtccccgaa ccggccgccc ggcggcctac ctgcgcttcc tggccgcggc
     7741 cttcacggcg aacctccagg cggtggtcga ataccggatc tccttcctga tccaggtctt
     7801 cggcatgatg ctgaacaacg ccgccttcgc cgccttctgg gccgtcctga tcgcccgcac
     7861 cggctccgta ggaggatacg ggttcaccga cgtgatggtc atctgggccc tgacctcgac
     7921 ctccttcggg atcgcccacg tgctttgggg caacgtccgc agcctcggct ccatcatcca
     7981 gaggggcgag ctggacgtgt acctcctcca gcccaaggac cccttcgtga acgtcctggc
     8041 ctcccgtacc atcgtctccg cctggggcga cctggcctac ggctacctcg tcctggcctt
     8101 cctcccgggg acggggcccg aacgcctggc cctgttcacc cttctggccc tgggaggggc
     8161 ggtcatcttc gccgccgtct tctcagccgc cgagagcctg accttcttcc tgggcaacgc
     8221 gggacccctg tcccaggcgg tgacggagtt cctgatctcg ttctccctct atccggagac
     8281 catcttcgat tccgggatgc gctggatctt ctataccatc cttccgtcgg gattcatcgc
     8341 cttcgtcccc ctggccgtgt accggagctt ggactggccc ctggtgcccg ttctgctggg
     8401 agtggcggcc gtctacgccg gcggggcgta tgggctgttc cgggcgggcc tgcgcagata
     8461 cgagtcgggc aaccagatgg ggacacggtt gtagcaggcg cgcccggcca ggaagccccg
     8521 ggcgggcctg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntggggaca
     8641 cggttgtagc aggcgcgccc ggccaggaag ccccgggcgg gcctgcgcag atacgagtcg
     8701 ggcaaccaga tggggacacg gttgtaagcc gggcccgccc gccggatccg ccggctcgat
     8761 tctccccggg gcgcgatgac gccgtgcgcg atagcgcaca cgtcgatcca ttcctacgtc
     8821 aaacgacgcg aagcgtccta ggagcctgtc ggacttgtgg atttttggcg ccgacaggcg
     8881 ctaaaaatcc cgattatccg gctcctttgg agtttgcaaa gtagaaaaat cacatagatg
     8941 cgattttcta ctattaaggt gggcaagccc aacaaactcc taggaccgag tgagaccccg
     9001 gggcccgcag cgtccgttcc catcccgcgt tcggcgcttc ccgccccccg ggagaagccg
     9061 ccttccgaaa tccccgcgcc cggcgccgcc ggtcgatcgt gaagcttcgt aaatttaggc
     9121 gggaagcgcc gaaccaggag aagaccgcaa gggacgcgga ggaccccggg gtcgaacgct
     9181 tccgcagcgc cccgccgagt agccgatgac cgtcgatgac aggtgaagct tccgctttaa
     9241 tgcaggagag gcgcgaggac cgagtgagac cccggggtcc gcagcgcccc gtgcccatcc
     9301 cgcgttcggc gcttcccgcc cggaagccgc gacgctttcc ggttcccgtc ccgacgccgg
     9361 gcgcccgctt tcggaaaacg gctgaaatat cacttgacgt actgaaatat cagagggaaa
     9421 atagcgccaa ctggagcata ccgtgaacag aatcgtcgat catcctatat tatccgcccc
     9481 ggaaggccgg acctccgtga ccttctcctt cgacggggcg gccctgtccg gcttcgaggg
     9541 cgaggccgtc tcatcggccc tcttcgcggc gggggtccag accttctccg tccacccccg
     9601 gggggacgca ccccagggga tattctgcgc caacggccag tgctcccagt gcacggtcct
     9661 gatcgacggg gtggcccgga aggcctgcat cacccccttg gcggagggga tggatgtccg
     9721 gaccctccac gggttcccgg agctcccggc acgggacgcc cccagggccg ccgtctccgg
     9781 ggagaccctg tccacggacg tcctcgtgat cggggggggc ccctcggggc tcgccgcggc
     9841 ggcggagctg gcccggctgg gcctgtccgt catcctcgcg gacgacaagg tctccctggg
     9901 cgggaagctg gtcctccaga cccacaagtt cttcggctcc gaggaggact gctacgcggg
     9961 cacccggggg atcgagatcg ccaaacgttt agaggaagac atccgctccc tcccgggtgt
    10021 gacggtcctg gagaactccc cggtggcggg cctctacaag gaccgcaagg cgggggtctg
    10081 gcggaactac gaatcctacg tcctcgtgga cttcaaggca ttggtggtgg ccgcgggagc
    10141 ccgggaaaag agcgtcctct tcccgggcaa cgacctgccg ggggtctacg gagccggagc
    10201 cttccagacc ctggtgaacc gggacctggt gcgggcggct cggcgggtcc tcatcatcgg
    10261 gtccgggaac gtgggcctca tcggggccta ccacgccctc caggcgggaa tcgaggtggc
    10321 cgggatcgtt gagatcctgc ccaaggtgaa cggctacaaa gtccacgcgg acaagatcgt
    10381 ccgcatgggg gttccggtct accttaacac cacggtggtg tccgtggagg gaaacgggaa
    10441 ggtcgagcgg gccacggtgg ccgaggtcga caaggactgg cgtccgatcc tgtccaccgc
    10501 ccggacctac gaggtggaca cggtcctggt ggccgcgggc ctctcaccct gcgacgagtt
    10561 ctaccgccag gcggtcgatt tcgggttcct ggcggtcacc gccggggacg cggaggagat
    10621 cgcggaggcc tccagcgcca tgttcggcgg ccggatcgcg gccctgagcc tggccaagct
    10681 actggggaag aaagtggaca tcgacgagtc ctggctagcc aagcgggagg tccttaagtc
    10741 ccggcccggg gacaacctct gccgggacga ggagatcccg acggaattct ggaagcccgt
    10801 attcttctgc accgaggaga tcccctgcaa tccctgcacc acggtgtgcc ccacccactc
    10861 gatccagctc aaggagcgca aaggctccat catggacctc ccctacttcg agggatcctc
    10921 ctgcaagggc tgcacggtct gcgtggccgc ctgcccgggc ctggccatgt ccctcatgcg
    10981 ccgcgtagac gagggttggg ccgaggtggt cctgcccttc gagttcaagg cggacttcga
    11041 gcccggagcc cgcctgcccg tcctggacca ggagggggcc tttttagagg acgccgagct
    11101 cctcaagaag gtcttcaacc ggaagtaccg cacctggatc ctcaccctca aggtcagccg
    11161 ggcgaacgct ccccgggcca tcggcatccg ggtccagtcc gaggcggcca cccggcccct
    11221 gccggcgccg aggttcgagc acctgccgga cgacgcggtc gtctgccgct gcgagcggat
    11281 caccgtgggg gagatcgtcc gcttcatccg ggaaaacgcg gtcaccgacg tgaaccagct
    11341 caagaccctg cgggtgggca tgggggcctg cgggtccaag acctgttcgg tcctcctgcc
    11401 ccaggtcttc cgcaaggccg ggatcgatcc cgcccggacc acagcgggca ccctccgccc
    11461 cctggccctg gagatcccca tgaaggacct gatcaacgaa gccgccggga ggcccctgtg
    11521 aagacctgtg acgtcgcgat cctgggcgcc ggaagcatcg gcgtgcccct ggcctactac
    11581 tgcgccaaaa agggactttc cgtcctggtc ctggacaagg agccctcctg ggggcggggc
    11641 cagaaccggg ccgccatcgg cggcatccgg gccacccact cggaccccgc caagatcaag
    11701 atcggccttc tgtccctgga catcgcccgg aacatgaagg aagagcacgg cctcaacatc
    11761 gagtggcacc agggcggcta catgttcgtg gcctacgacg aggagcggga gagatccttc
    11821 aaggacctcc tggtcgtcca gaaggccgcg ggcctcgaca tcgactggat ctccccggac
    11881 cgggtcctgg agctctcgcc gggcctcaat cccgagggcc tgcggggcgg aacctacagc
    11941 cccggggacg gcttcgcctc ccccctcatg acctccacgg ccttccacaa gctggccctg
    12001 gacgcggggg cggagttccg cttcggcgag accgtgacgg gcttcgggat cgagggccgc
    12061 cggatccgga ccgtccggac cgacgccggg gaatactcgg ccggcacctt cgtgaacgcc
    12121 gccggcgccg aggccgcagg cctcgggaac ctggcggggc tcgagctccc cgtgcacccg
    12181 gacctccacg agggcggggt cacggagccc gtgcgacgct tcatggagcc catggtcgtg
    12241 gacatgcggg gggacgaccg gtcggggaac tactacttct accaggcggg caccggccag
    12301 gtgatcttct gcatcacccc caagccccag atctggggga ccgacacgga caatacctcc
    12361 gccttcctgc cccactgtgt cagccggatg gtctccctct acccccgcct gcggaacctc
    12421 aaggtccgcc ggacctggcg gggctgctac cccatgaccc cggacggcct gcccgtcgtg
    12481 ggctatcccc gggagccgga gaacctcctc ctggccgtgg ggatgtgcgg ccagggcttc
    12541 atgctcggcc cggggctcgg gaggatcctg gcggacaccc tcaccgggga cggggcgcgg
    12601 tacggccagg tcttcgagga gctgtccctg taccggaagt tcgagggcat ggaattgttg
    12661 aagtagggcg cggccgcgcg gcgtcgcata cgcttaataa aacgaaggcg cccggggaac
    12721 ccgggcgcct tttccatttg atgatggttt cttggctttt ttaaatggtt tttcgatcgt
    12781 tctttctggt ttcttcaccc ggccggccga cggagccctt cggctgcgca tcgcccgaca
    12841 gaatcaggct accaccgccc cggagggttc gcaagttgaa atgttgattc gacttgtaga
    12901 catttgtcta caagcccgat ttggcccggt tttcgggccg caatccgcgt ctacacgttg
    12961 tagacgcgcc caaaaatctc tattgacggc tagttccggc atgatatact ccctccatgc
    13021 agttcccgga cgccgattgg gagcgcctgt gcggcctcgt cgccgaagcc ttccggatgg
    13081 acggctccga gcgcgcccgc ctggctggtt cccgagcagc tcggatcacc ggggccctgc
    13141 cctacctggc gggctgccgg aatcccgagc ggacggccct ggcccacctg gccgccttcg
    13201 tcctggcctg ccgaggcggg acccggaagg tcttcgacca cggaccggcg gacgacgcgg
    13261 agcccctggc gcgcctggag cccatcgccc gcttccccgg cggggacccc gcggtgatcc
    13321 gcaagggcat ggcccttctg ggtctcctga tgctcggggg ctacgaacgg gaccgggaga
    13381 aggacaccgc gggcggggag tacaaccccc tgaactccgg ggcgtggaag gccggggaag
    13441 tccgggcgag gcttcaggcc gaggctgcct ccgccaaggt gccggaactg gacgcgatca
    13501 tcagcaccga cgaggcgtcg gtaagggggt actgggatgg ctgagacaaa gaccgcgatc
    13561 aatccgctgg ctcgtatcgg gaggttcacc gccctggtgt tcgtggcggg aagcctgctc
    13621 atgttcgtcg tttggatgtc ccggggatgg ggcttcgtca agaccataac ccacccgtac
    13681 atcgggattc agcttctggt ggccttgctc ttttacatca gctcccgcag cccgaagctg
    13741 tacatcatcc aaccgatcct cttattgata caagctccga tcaactttct ggattcccat
    13801 gactccttct acggcctcgg gtttttcgtg atggcggtcc ttctcctgtt caagatcggt
    13861 tttttcgaac ggggccggat cccgaaggtc atcgggctcc tggtgtatct gtacggatgg
    13921 gagctgttcg cggcgatccg ggccggaaga cataccgggt tgtccctgac cccggtgttc
    13981 ttcgtgacgg tcttcctgat cttcctctac atcctgtaca aggaacaggt catcgtctac
    14041 ctgaagcagc ccaaacccct cctggatctc caggccaagg gactcgcgga caccgaggcc
    14101 gcctacatcc tggccctggc caagggcctc aacgtcaagg agatcgccgg gaaattcgag
    14161 gtctccgagt ccacggtccg gaacaccctg gcccgggcct acaagaagct gggggtccag
    14221 gacaaggccg agctgtccac cttcctggcg gaatacaccc tgcccgaggg ttgaacgacc
    14281 gcgggccgat cccaatccgg cgccgctccc ggaattccgc gcgccgaacc cgcgcccgag
    14341 aacggaggca tccatggcgt tcttcgaacc cttccggctg gagcgcttct tcgcccgctg
    14401 ggagttctcc gccccgcatc tcctgtgctg ctccgactgc gagagcgtcc cggtctccga
    14461 cctggcggcc ctggatccgg gccttccgga gcgcctttcg tccctgcgcc tgggctacac
    14521 cgagacccgg ggcgccccct ccctccgcga ggcgatcgcc ggggagtacc ccggggccgc
    14581 gccggaggag atcctcgtcc acgcgggggc ggaggaggcc atcctcaacc tctgcctggc
    14641 cctcctttcc aagggcgacc gggtggtggt gaataccccc tgctaccagt ccctggcgga
    14701 gatcccccgg gccctgggct gcgaggtcct gccctgggag tggcgcccgg ccggaggccg
    14761 ctgggccctg gatcccggcg acctggaccg catcctccgc tcgggacccg atccgaagct
    14821 cgtcgtcctc aacgcccccc acaatcccac cggggggttg ccgaccaagg cggaattcga
    14881 cgcgatcgtg gccgcggcgc ggcgggtcgg ggccgtggtg ctcatggacg aggtctaccg
    14941 ccgcctggag cacgaccccg cccggcgcct gccgtccatc cgggacacct acgagaacgg
    15001 ggtggccctg gacgtccttt ccaagcacgc gggcctggcg ggcctgcgga tcgggtggct
    15061 ggccacccgc cgggcggaca tcctggacgc cgtggccgtg gtcaaggact acaactccat
    15121 ctgctcctcc ggccccagcg aggtgatcgc cgagtccgcg gtccgcaacc tttcggccct
    15181 ggtggaccgg aaccgccgca tcgtggcgga caacctcgcc ctcctccgcc gattcttcgc
    15241 ggcccggccc gacttcgccg agtggaccga gccggagggc tcctccgtgg gcttcccccg
    15301 cctggccttc ccgggggacg ccgaagtcct ggccgagcgg ctggtgcggg agaccggggt
    15361 cctcatcctg ccgggatcct actacggctg cgacgcgcgg cacttccggg tggggtacgg
    15421 tcggatatcg atgcccgagt ctctcgcccg cctggaaaaa tggctggacg aaggcggccc
    15481 ggccgccgcc gctctttcct gagtcctgag tcctaactat ccgcgttccg gtacagtttc
    15541 cgccgcccgg cccggccctc ctcccggagg aaccccgccc gggccaggga atagaggacc
    15601 ttccgcgccc gggcaggggc gatccccagg gagcgaccca gggtctcgga gctccagggc
    15661 tccgggaggt cccgggggat cagggccagc cagtcctccg gggattccca gctcgcggcg
    15721 ccgacgatcc cctccaggaa ccggtcctcc gtgcggtcgc cccgcttccg ccaggagccc
    15781 gtcccgtccc gcacccggac ttccgaggcc cgcaccagga ggacctccac ggccacgccg
    15841 ggcagggaga tcaggtccgg ggccttcacg agttcgtcga agacgctcca gaggtctccg
    15901 cgcttggggc ttctccgggt cgacacgatc cggtcggaat cagggtcccg gcgccggatc
    15961 tccagggatg ccgggacggg atgcactacc cggacccgga tgcccgctcc ggccagggcc
    16021 cggaccttgg gcaggagggc gtagaggtgc cgggtctgga cctccacgac cgagccgtcc
    16081 gaacggagga ggtcgaccac ccggcctccc acggccgcct cgatccggtc ccccggaccg
    16141 gccaagaggc gcttcacctg gtcgtggagg gaccgctcgg aatagaggtt gatgcccgag
    16201 cgttccgccg cccggagggc gctcggaccc tccggactca ggaccattcc agctccgcgg
    16261 cgatctcgtc gaccaggtac acgggcgggg cctccgggac gtcccgccag gcctcgtcgt
    16321 tggtcaggat cgcctccgcc ctgtggacca gggccgttgc caagtgcacg gcgtccgcga
    16381 aggagacccc gaagcgggag gatcccggat ccccgggacg gagccccgga agcttccggg
    16441 accgggggtc gaaggtgccc tcccccagga tgcgggccgc ccgctcggcc acctccgcgt
    16501 ccacgggcac caggaccagg agggaggaat ccgccaagaa acggcggtac cgcagggcta
    16561 gttcccggtc cccccgggac aacggccgct ccagaagctc cgtcagggtc aggaccgagg
    16621 cgaagggccg ccctcccgcc gagcgcaccc ggtccaggaa gtcggagacc gcggggccgc
    16681 ggcgggggac gtcctccacc aagtacacca gggcggagga gtccacgagt atccgggagc
    16741 ccggggacag gtcgagatcc atggaagaaa gcatagccgc ccgcccgggc cttgtcgata
    16801 cgcgttcccg gtgtcacgat gcttgtgaca gccgggcgga cggagacctt cacgagcttc
    16861 gtaccaccag gtccgggttg cggataccgg cccgccgcgc tatccttcca ggcggggcgt
    16921 ccccgccccc caaaggagct tccatgaaga gtctcatgcc cctcgtcctc ctcgcgatca
    16981 tcctggccct cctggttgtc acgaacccca ccaagcagga cttcgccgcc tggtacggaa
    17041 aacaagcggc ggcctccgcc ccctcgggca ccctgggcaa ggccctgggc ggcatcggca
    17101 agggcatggc ctccgtggcc gcctcggcct tccagcggca gagctacggg gtcctgagcg
    17161 tcttcagcct ggccaaaggc ggtaccgcct acgtcgggct gggcaaggtc gtcttcctca
    17221 aggtccgcta gcgggcttgc cggaaccccg gcccttcgcg atttcctgac acaattctgt
    17281 tgcgcggcta acgatccgga cgtacactgg taggtgctct cccgccgctc gcaggcgggt
    17341 attacgaaca ggaggctcta gcatgaaacg gttttccctg ctgatgctcg ccttgcttct
    17401 ctgcgcgtcc acgatcttcg cgcagatcca gatcgactgg tggcacgcca tgaccggcaa
    17461 gaacggtcag atggtggagg agctggccaa gcgcttcaac gacagccaga ccgcctacaa
    17521 ggttgttcct gtctacaaag gttcctacag cgacaccatg aacgcgggca tcgcggcctt
    17581 ccgggccaag caggcccccc acatcatcca ggtctacgag gtcgggaccg ccaccatgat
    17641 gaacgccggg ggagccgtct acccggtcta caagctcatg aaggactcgg gggagaagtt
    17701 cgatcccaag gcctacatcc ccacgatcac cggctactac tccacctcca agggcgagat
    17761 gctctccctg cccttcaact cctccacggc ggtcatgtac tacaacaagg acgccttccg
    17821 caaggccggc ctggaccccg agaagccccc cgtcacctgg ccggagttct tcgaggtcgc
    17881 caagaagctc aaggcctcgg gcatggaggg tggcttctcc accaactgga tctcctggat
    17941 ccagctggaa aacttctccg cctggcacaa ccagagcttc gggaccaaga acaacggttt
    18001 cgacggtctg gacacccagc tggtgttcaa cacccccctc acgatcaagc acttcgagac
    18061 cctgtacgaa ctgtccaagg acaagacctt catctacggc ggacgcgaga acaaggccaa
    18121 ccccttgttc acctccggca ccgtgggcat gcacttcgag tccatcggcg ggtacgggaa
    18181 cttcaagaac accgccaagt tcgatttcgg cgtagcgcgc ctgccctact accccgaggt
    18241 caaggacgcc ccccagaaca cgatcatcgg cggcgccagc ctctgggtct tcaacggcaa
    18301 gcccaaggac gactacaagg gcgtggccaa gttcttcacc ttcctctccc agcccgagat
    18361 ccaggccttc tggcaccaga gcaccggcta tgtgcccatc accgtggcct cctacgagct
    18421 gaccaagaag cagggcttct acgacgccaa ccccggtccc gaggtcgcca tcaagcagct
    18481 gctgaacaag gcccccacgg gcaactcccg gggcatccgc ttcggtaact tcaacatcat
    18541 ccgggagatc gaggaccaga cctgggagga catcctggcg ggcaagatct ccgtgcggga
    18601 cggcctcaac aagatggtcg ccgacggcaa cgcggaactc cgcaagttcg agcggctcta
    18661 caagaaatag accccttgcc aaggggtgcc cggcgcgacg cgccgggcgc ccgcaccggg
    18721 gccgcggctc cccgcggccc ccacctttcg caacatcggg atacgccatg gctaagcaat
    18781 acgagttccg gatcaagggc ctgccctacc tcctggtcct tccccagatg gtcatcgtct
    18841 tcatcttctt cttctggccc gccgcccagg cgatctggca atccttcttc ctccaggacc
    18901 ccttcggggg acagtacatc ttcgtgggcc tggagaacta ccaggccctg ttcacggacg
    18961 aagccagcgc ctacgcccaa tccttctggg tctccgtgtt cttcgccgtc agcgtgacgg
    19021 tcctggccat gtccctatcc ctgttcctgg cggtccaggc caacaagaag atccgggccg
    19081 cctcgttcta caagaccatg atgatctggc cctacgccgt ggccaccctg atcgccggcg
    19141 tcctgtggct cttcatgttc aaccccaacg tgggggtcgt ggcctggttc ctcaagcatc
    19201 gcctgggcta cgagtggcag cacctcctga acaccggaca ggccctgacg ttgctcatcc
    19261 tggcggccag ttggcgccag atgtcctaca acttcgtctt cttcctggcg ggcctgcaga
    19321 gcgtgcccaa cgacttcatc gaggccgcgg ccatcgacgg ggccggaccg gcccgccgct
    19381 tctggaagat catcttcccg atcctctcgc ccaccacctt ctacctcctg gtgatgaacc
    19441 tggtctacgg cttcttcgag accttc
//
LOCUS       DAOTEI010000050        19337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1864_length_19337_cov_4.826678, whole genome shotgun sequence.
ACCESSION   DAOTEI010000050 DAOTEI010000000
VERSION     DAOTEI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 19337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19337
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_19337_cov_4.826678"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="P5313_06755"
     CDS             <1..250
                     /locus_tag="P5313_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80096.1"
                     /translation="SVSEALRIQEELGKIDIPLSGLCVNKRGVSKADWSLDKRLAGIR
                     AFDFTFLTGGLHEREDLRTIETEALVADFLASGPKGRA"
     gene            247..897
                     /locus_tag="P5313_06760"
     CDS             247..897
                     /locus_tag="P5313_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80097.1"
                     /translation="MNLNPYLLSALVVMGVAATAQYFFGRRRNKALAASISRGLEALL
                     KPNHTNYVNIGGSIGYNFVYTLSTGPWVSAKGTMTLSPRQSLLYLPISRLLGFTDRFF
                     INLFTKKKIKGEAHIVSRTTLRRARIDGIEEMNRRDAEAGGKRFVLLWRGTDLSSELE
                     SLLAAFPEPGRLRHFCSFPDNKTFYLYTVPRGGDASADLETVLHAVPRFLDSKKEA"
     gene            900..1178
                     /locus_tag="P5313_06765"
     CDS             900..1178
                     /locus_tag="P5313_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80098.1"
                     /translation="MELDPATRAKFDAVLARVKDPVSELALSELNLVSKIRYSAKEKA
                     LVVRLDAAPRAHECPACSAMNGQIIVTIERTLREELQKEFPGFTVEYE"
     gene            1436..2497
                     /locus_tag="P5313_06770"
     CDS             1436..2497
                     /locus_tag="P5313_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80099.1"
                     /translation="MKRLIPILAIGVLLAAGCATKPPAPEPEPVPQPAPTPSVSQAEA
                     DALLAEATALRKKAFDLKLFEVVADDYKAADAKYVEGKTAYDAKDNEKAAPVLKETIA
                     AFKDVLAKGAGILAAENRKRADEMKALAVKEGAPETAAERMAQGDAAYAAGASLSEAK
                     KDEEALVAFEKARQTYEAAYKKARALGIRDSIEEKDFARYDAGNFKLADEKLAESDAL
                     FATNPDGALDALDEATLRYNLVVQKGWQTYAVERKAPADDAKRRSEEIKAQVAVKEKY
                     AEALAVYNNASALLAGGNYQEAGPEFDRSAELFEEAYAEAQVKRDAALAALAEAEART
                     QESESKARSGDEILGTAEE"
     gene            2508..3200
                     /locus_tag="P5313_06775"
     CDS             2508..3200
                     /locus_tag="P5313_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80100.1"
                     /translation="MKRATLLIVALLIAASVFGQSLLQNSYYRKSLELRAQAAEAFEA
                     GDYDAAADLAAQAQEFARLSDEFVETMLAKNQADQAYAAAQVRMTWAVTNNVPDTYPS
                     EWSLATARMALAAAAYESESWLDAAEYSDLVLEALADVTERLPLPRYYAVRLIPERRD
                     CLWRIAEYPFVYGDPWKWRLLYDANKKVLVNPNDPDLILPGQILEIPSIRGETRSGTW
                     DPTKPYVELPKP"
     gene            complement(3277..3603)
                     /locus_tag="P5313_06780"
     CDS             complement(3277..3603)
                     /locus_tag="P5313_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY80101.1"
                     /translation="MEPTIPRKYSLRAEMFKALAHPARIYILDKLRERPRCVCELAAE
                     IGLEKSAASKHLSQLKEAGFVEDERRGTLVEYRLVAPCVLDMAACAEGTILENRRKRL
                     EIPGEG"
     gene            3694..3933
                     /locus_tag="P5313_06785"
     CDS             3694..3933
                     /locus_tag="P5313_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY80102.1"
                     /translation="MKIQILGKGCPNCELLAQRAREAVAELGLDAQVEKITDVDKILD
                     MGVMMTPALAIDGDVKRVGKVLAKSQIIEILKGGN"
     gene            3935..4306
                     /locus_tag="P5313_06790"
     CDS             3935..4306
                     /locus_tag="P5313_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HRY80103.1"
                     /translation="MAGCTCGGTCETNLLYACSGAANTGLLADQVMRALMRDKVGVGT
                     CLAAIGAGLSGYLQSAQSATRNIVLDGCPVACGAKIFQKAGIPFQHHILTEYGVEKGK
                     TPITGDVIEDVAARVAAKVRA"
     gene            4303..5487
                     /locus_tag="P5313_06795"
     CDS             4303..5487
                     /locus_tag="P5313_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRY80104.1"
                     /translation="MSGKAWTPNRKLLYLAGAFLAAYFIPWGAPRVTSAVQEALLMLS
                     EYAREHVLLCLVPALFIAGAITVFLNQQAVLRYLGPDANRVTAYGVASVSGTILAVCS
                     CTVLPIFKGIYKKGAGLGPATAFLYSGPAINILAIVLSAKVFGWRLGLARAVGAVLFS
                     VLIGLAMALIFRKEDERRAADARLFSGSQEKPSRGLGQMIVYMASMIGILVFVNWAGS
                     RGGSALWDAIHSAKWWISGAFALVLAYTVIRWFDRDERIEWVVATRDFALQILPLLFG
                     GVLVAGFLLGRPGRDALIPGAWIAGLLGGNSLLANLFASVAGALMYFATLTEIPIVQG
                     LLGSGMGQGPALALLLAGPSLSLPSILVIAGELGWKKTLTYVGLVVALSTLAGLAFGA
                     AA"
     gene            complement(5540..5902)
                     /locus_tag="P5313_06800"
     CDS             complement(5540..5902)
                     /locus_tag="P5313_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY80105.1"
                     /translation="MEIPDRERLLRRASILKSLAHPSRLLMIEMLEEAPRCVGELTEA
                     VGSDITTVSKHLAVLKRVGLVRDVREGQFSRYSIVCDCVSHLVDCIEEGMRDHGSPDE
                     IRPPGAGVRCSAGRSKRS"
     gene            6020..7108
                     /gene="arsB"
                     /locus_tag="P5313_06805"
     CDS             6020..7108
                     /gene="arsB"
                     /locus_tag="P5313_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRY80106.1"
                     /translation="MSDTPNAGLGFFAKYLSLWVALCIALGVAIGYFLPGVPAFLGRF
                     EYAKVSIPVAVLIWLMIYPMMLKVDFTSIRNAGRKPKGLIVTLVVNWLIKPFTMYLIA
                     WFFLKVAFKAFIPSGLSTEYLAGAVLLGAAPCTAMVFVWSYLSDGDPGYTVVQVAVND
                     LVILFAFTPIVAFLLGVSDVRVPLDTLILSVVLFVVIPLGFGALTRSRLIRKRGKEWF
                     DGVFVKKFDGITEGGLLLTLVILFAFQGEVILRNPIAILLIAVPLVLQTYFIFFIGYG
                     WAKAWKVPYEIAAPAGMIGASNFFELAVAVAVSLFGLSSGAALATVVGVLVEVPVMLS
                     LVRIARATRSRFPGAAEPEAELVPSRAK"
     gene            7136..7633
                     /locus_tag="P5313_06810"
     CDS             7136..7633
                     /locus_tag="P5313_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80107.1"
                     /translation="MSSRARVATDFSSVTGALPRTEGILNLTPAEAAEYLSKGAILVD
                     LREAYETNFRVFEVPQTIHLPWTLFSKGFERLPRDLPLVLADASGIYGREAARMLRNA
                     GFTNIAKMAGGMIDWDREGLPVRRDPLFELSGQCACKLKTRTGKNPLLDKNAVVIDPS
                     REESP"
     gene            7630..8052
                     /locus_tag="P5313_06815"
     CDS             7630..8052
                     /locus_tag="P5313_06815"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRY80108.1"
                     /translation="MKKVLFLCVHNSARSQMGEAFLKRLAPERFEAESAGLEPGTLNP
                     YVVRAMAEAGIDISHNPTKSVFDLHARGAAFDYVITVCSKEAAERCPIFPGRAERIHW
                     PFEDPSSFRGSDEEILARVRSVRDEIERAVRRFISERG"
     gene            8149..9456
                     /locus_tag="P5313_06820"
     CDS             8149..9456
                     /locus_tag="P5313_06820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY80109.1"
                     /translation="MLDTGRLAADERRAYRIFLVSLLGALVVLFTGVFLGFTARNADF
                     LREVVLERAQSLFRQIVMTRQWASEYGGVYVRKGPGVESNPWLEHPDLQAADGSLLTL
                     RNPALITREISEIASVRDDYRFKITSLNPLNPGNIPDDFERRALRSFEAGALEYWETT
                     DGQAGREFRYMGALKTERSCLACHAKQGYREGDIRGGISVTFPVDRLEGELKKNVALV
                     FWLELLIAAGTLAAVLFFVIRLRRQLDRVRSELEQAATRDALTGLYNRRYILERFVQE
                     ESKCRRSGQPLACALIDADDFKAVNDRFGHAAGDAALRIIAENLKSGVREYDIPGRFG
                     GEEFIILFPGVRAAEARIACDRIRAAIEASPRPPVLETARLTVSVGISDTVCSRGAGT
                     NRDSLDSLLFRADEALYRAKSGGKNRCETCPDESGSPDREPGT"
     gene            complement(9712..11367)
                     /locus_tag="P5313_06825"
     CDS             complement(9712..11367)
                     /locus_tag="P5313_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRY80110.1"
                     /translation="MRKRLVMTLFAVLALSAALGAQETAVSRVVRVTVYGDRALVTRR
                     AEVSLFKGESTVLFGNLPASVDPASVQVSGKGAFTLRDVRVAARQLTRDVSAQLTALE
                     TEKRGLEDKLTLVNDRVREAEAERTFLADMAKRLTSNAGNSESLPLDPGSWAKMLDFH
                     RQRNAAVNETVRTSRREIQALQAEIDRVNREIRSLGAGTRLSVTEAELVLDASSPTKA
                     VLEVSYLVAGPSWRPDYVLRADSEAARLSVHYRALVRQNTGEGWEGAELFLSTARPQA
                     GGALPVLPPWYLDIYSPPSARRDEAAKSAPAPAAAGVRGAAESSSDLAWGEPAPEMRI
                     DTAQASTGATAVTFAIPGATTVSSDNKDRTVTIAVLDLPVTFSYAAIPKLSPYAYFRC
                     EVKNDSAYPILAGPSHVYVDGSYVADAALEAVPAGGSFKADLGIDEGIRVERKLLRKF
                     DENTGALTKKSKTTWEYEIKVKNSKKRDIVLEVSDQLPVSLNEQIVVKALAPPYSKDT
                     DTLRKIENETFVWTLKLPAGKESVLPLSFSVEYPRGMPILGLE"
     gene            complement(11414..12727)
                     /locus_tag="P5313_06830"
     CDS             complement(11414..12727)
                     /locus_tag="P5313_06830"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HRY80111.1"
                     /translation="MPAVSAKSLCDFLDASPTQFHAADALAAALQARGALALDERSEW
                     NLEPGRTYYVVRNGSGLVAFRIGGESPARTGFALAGAHTDAPALRVRPEKSLRHRAGD
                     RIAVEVYGGPILSTWLDRPLALAGRAAVRGSDGRPELRLVNPAGPAAVIPNLAIHLNR
                     EVNKGFEYNVQNHLPALVAAPLADRRPAEGDAPWALAETARNLGVPVRDILGAELFLV
                     DASRAVILGRDGELVNSGRIDNLAGCHAILAAFNAAPPGAHGQAAVFFDNEEIGSGTR
                     QGADGLFLRDVLARVCARSGCDSEGFYRALAASFCVSVDAAQGWHPNFQEKYDEAYSP
                     VLNGGPAVKANANFKYATDAESEARFRACCEDAAVPCQKFAVRADLTPGSTIGPLSSA
                     LSGIRTVDVGSPLLAMHSIRETAGSYDHDFMTAALRRFYEKGPGA"
     gene            complement(12783..13100)
                     /locus_tag="P5313_06835"
     CDS             complement(12783..13100)
                     /locus_tag="P5313_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRY80112.1"
                     /translation="MRSYSIGEVERVLGEKAHVIRYWERSVPLVSPRRSESGRREYSE
                     AELLALLRVKHLVRRRGLSPEAAREVLIREASGPRQDLRARAFEIRALLVRAYILSLN
                     SLP"
     gene            complement(13105..14508)
                     /gene="der"
                     /locus_tag="P5313_06840"
     CDS             complement(13105..14508)
                     /gene="der"
                     /locus_tag="P5313_06840"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRY80113.1"
                     /translation="MRETNPQEPAAPAAPAARTRYRNLPVVVVAGRPNVGKSTLFNRL
                     LRKRRAITDPTPGVTRDPVDAECVLPGTERRVLLVDTGGVTLERSDMNDLVSRRSVAT
                     LDKADLILFLVDATGLTPEDEELAARLRRRADRTILVVNKTDSPERDALAYGFARLGF
                     SELVAVSAEHGRNVDELVERIVARLDFSKVSSEEDLHDDVRVAILGKPNTGKSTLLNR
                     LLGEQKSLVSEIAGTTRDVVEGSTTWKGRRITVLDTAGIRRKKKVHEDVEYYSVNRAI
                     RTVEDCDIVVLMIDAQEGLTDQDKKIAALAVEKGRGLVFALNKWDRMPDLKNSFEASR
                     DKLRYFFGQMAFAPVLPLSAKEGTGVPKLLDMLLAVFGQLTRKIETSALNKAVEDWVA
                     AYPPPVGPRTRFKLRYAVQSGINPVKFILFTSRPDAVGESYLSYLKNKIRSDLGFSLI
                     PVGLEVRGSSSWKADRA"
     gene            complement(14562..16304)
                     /gene="ptsP"
                     /locus_tag="P5313_06845"
     CDS             complement(14562..16304)
                     /gene="ptsP"
                     /locus_tag="P5313_06845"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRY80114.1"
                     /translation="MIELTGVPVSPGIAIGKAFLFLEDEDVSIPNYYIRTADVDSEWD
                     RFRTAIGKARGEITELRDRARSEMGPTHSAIFDAHLLMLEDPELMEGLESSLRESLRN
                     IEWIVFQREQELIEQLSRVQDPYIQDRVADIRDVTKRILNHLMFRERFTLSDLEDEIV
                     LVAHNLLPSDIIAMNKRAVKALVLDGGGKTSHAAILARAFEIPAVLGVAGATRNVRNG
                     DGVIVDGRKGRVVVEPDESTLIRYEQVRSRDVEREKALLATEVLPVRTKDGRSILVKA
                     NIEIPEEVDTVRRHGADGVGLFRSEFLFLQPGRIPSEEEQYRAYRSVLKSMEGRPVTI
                     RTLDLGGDKALPELSGREEKNPLLGWRAIRLCLSRDDLFLTQLRAILKASVEGDARIM
                     FPMISVPEELDQALALLERAKAECRAAGFAFDENIPVGIMIEVPSAALVADILAKKSA
                     FFSIGTNDLIQYTLAVDRGNEKVAYLHQPFHPAVLRLIKATIEAARAAGISVGMCGEM
                     ASDPYAALVLLGLGLDEFSMSSVSIPEIKRLLRGADSGEAASLAREVLAMESAAEIER
                     YLRERIDPEAGVFR"
     gene            complement(16372..17328)
                     /locus_tag="P5313_06850"
     CDS             complement(16372..17328)
                     /locus_tag="P5313_06850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY80115.1"
                     /translation="MRAARFFHRDPLEAFDGRGTFAAVSSALVFAALFLGSPASLPDA
                     AVSVGSADMRTLALETLEAEAGAELIAETPPRDEGLWYQVYEVKKGDIPGMIAEAFGV
                     TVDTLISFNDIRNTRAIRIGTHLKIPNMNGILHEVTAGDTVESIAAKYSVPADRVAEV
                     NRVGPGTLFEGRKLFLPDARLSSFTLREINGDLFKWPIRGWITSWYGWRDDPFSGTRT
                     FHTGIDIGAPHGASVRAAMEGRVSTTGYNAVSGNYVIISHHSGYASMYAHLDSIAVRK
                     GQAVSIGTRIGAVGNTGYSTGSHLHFTVLKNGRTVNPMLLLN"
     gene            complement(17441..18598)
                     /locus_tag="P5313_06855"
     CDS             complement(17441..18598)
                     /locus_tag="P5313_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRY80116.1"
                     /translation="MKTREVLWIALAALALSAGAQTPGEASALTLAPEDLRIEQRADG
                     GYHLYIRAKPGLGSVLLTETTKDPAGKADNYAYRAPEKNPVNGDEKRLLDGKFLDPAR
                     GIWSLIDSTPEPDPELGSAFHIFLPWVVEYGYPWSRQGEVFLSDGTFVNIRAFPKPYA
                     DYTGGFRDNPYEIRVTQKPFPRPAPPPTPPAEPDKSLYMKETVRAFEAIAQAGKGDAL
                     FSTGREDINDKLGKILDTARGKSVDLVLCLDTTDSMKDDIDAVKESLPAMIRDRISEF
                     RSFRLGLVLYKDYFEDYLTKRYDFTADFGAFTANVTAVRVAGGRDIPEAVYEALHEAA
                     TAFPWSAEARMIVLIGDAPPHPLPRGKIDKAAAQAAALDRGIEVSVIILPH"
     gene            complement(18897..>19337)
                     /locus_tag="P5313_06860"
     CDS             complement(18897..>19337)
                     /locus_tag="P5313_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRY80117.1"
                     /translation="PVVPAALGEVESFYDPPFRRAIRADVAVVMLSHARFPALDPEAP
                     ASLSPAVIGRLKEGLGFRGVVLTDDVAMKALSGEGGAGDAAVAAVRAGADMVMASGGR
                     VAREVYRALLEAVRSGELREARVRDAAARILTQKLRFGLLKGID"
BASE COUNT         3136 a         6498 c         6481 g         3222 t
ORIGIN      
        1 ctcggtctcc gaggccctcc ggatccagga ggagctcggc aagatcgaca tcccactctc
       61 gggactctgc gtcaataagc gcggggtgtc caaggcggat tggtccctgg acaagcgtct
      121 ggccgggatc cgggccttcg acttcacctt cctgaccggg ggcttgcacg agcgggagga
      181 tctccgtacg atcgaaaccg aggctctcgt ggcggacttc ctggcctcgg gaccgaaagg
      241 acgcgcatga acctgaatcc ctaccttctg tccgccctgg tcgtgatggg cgtggccgcc
      301 accgcccagt acttcttcgg tcgcaggcgg aacaaagccc tggcggcctc gatctcccgg
      361 ggcctggaag ccctgctcaa gcccaaccac acaaactacg tgaacatcgg cggctccatc
      421 ggctacaact tcgtctatac cctgtccacg ggcccctggg tcagcgccaa gggcaccatg
      481 accctgagcc cccggcagtc cctgctctac ctcccgatct cccggctcct gggcttcacg
      541 gaccggtttt tcatcaacct cttcaccaag aagaagatca agggcgaggc ccacatcgtg
      601 tcccggacga ccctgcggcg ggcccggatc gacgggatcg aggagatgaa ccggagggac
      661 gccgaggcgg gcgggaagcg cttcgtcctt ctctggcggg gaacggatct ttcctcggaa
      721 ctcgaatccc tgctggccgc cttcccggag cccggacgcc tacggcattt ctgttccttc
      781 ccggacaaca agaccttcta tttgtacacg gtgccccggg gcggggacgc gtccgcggac
      841 ctcgagaccg tgcttcacgc cgtcccccgg ttcctggatt ccaagaagga ggcgtaacca
      901 tggaactcga tcccgccacc cgggccaagt tcgacgccgt cctggcgcgg gtcaaggacc
      961 cggtgagcga gctggccctc tcggagctga acctggtctc caagatccgg tattccgcca
     1021 aggaaaaagc cctggtggta cgcctggacg ccgccccccg ggcccacgaa tgcccggcct
     1081 gctccgccat gaacggccag atcatcgtca ccatcgaacg gactctccgg gaggagctgc
     1141 agaaggaatt cccggggttc accgtggaat acgagtgaat ccggggccgg agttcccggc
     1201 cccgtctccc ccgagcgaag cagccggcca tcccatcccg atggaaggga aacctctttc
     1261 cgggttttcc gcgcttttcc acccttcgaa gtttgccaga tcctttgcgg atgtggtact
     1321 atggatagta ggtaccagct tactcgaagc ttctgcactc tccgaactcg ttcgttccgg
     1381 cgattcccgc cggagcggcg tgtcgaacga taacaacaaa atcctggagg tgcttgtgaa
     1441 gagactgatc ccgatcctag ccatcggcgt gctgctggcc gcgggatgcg caaccaagcc
     1501 cccggctccc gagccggaac ctgttcccca gcccgccccg acgccctcgg tttcccaggc
     1561 cgaggcggac gcattgctcg ccgaggccac ggccctgcgc aagaaggcct tcgacctcaa
     1621 gctcttcgag gtcgtcgccg acgactacaa ggcggcggac gccaagtacg tggagggcaa
     1681 gaccgcctac gacgccaagg acaacgagaa ggccgccccg gtcctcaagg aaaccatcgc
     1741 ggcgttcaag gacgtcctgg ccaagggagc cggcatcctg gccgcggaaa accgcaagcg
     1801 ggccgacgag atgaaggccc tggcggtcaa ggagggagcc cccgagacgg ccgccgagcg
     1861 gatggcccag ggagacgcgg cctacgcggc gggtgcctcc ctctcggagg ccaaaaagga
     1921 cgaggaagcc ctggtagcct tcgagaaggc ccgccagacc tacgaggccg cctacaagaa
     1981 ggcccgggca ctgggaatcc gcgattccat cgaggagaag gacttcgccc ggtacgacgc
     2041 gggcaacttc aagctggcgg atgaaaagct ggccgagtcg gacgccctct tcgcgaccaa
     2101 tcccgacgga gccctggacg ccctggacga ggccaccctc cggtacaacc tggtggtcca
     2161 gaagggctgg cagacctacg cggtcgagcg aaaggccccc gcggacgacg ccaagcgcag
     2221 atccgaggag atcaaggccc aggtggccgt caaggagaag tacgccgaag ccctggcggt
     2281 ctacaacaac gcctccgcgc tcctggcggg aggaaattac caggaagccg gtcccgagtt
     2341 cgaccgctcc gcggagctct tcgaggaagc ctacgccgaa gcccaggtca agcgggacgc
     2401 cgcgctggcg gccctggccg aggcggaggc ccggacccag gagagcgaat ccaaggcccg
     2461 gtccggggac gagatcctcg gcacggcgga agaatgagga ggcggccatg aaacgcgcaa
     2521 ccctgcttat cgtcgccctg ctcatcgccg catcggtctt cgggcagagc ctgcttcaga
     2581 actcctacta ccggaagagc ctggagctta gggcccaggc cgcggaagcc ttcgaggccg
     2641 gagactacga cgcggcggcg gacctggccg cccaggccca ggagttcgcc cggctctcgg
     2701 acgagttcgt ggagaccatg ctggccaaga accaggcgga ccaggcctac gcggccgccc
     2761 aggtccgcat gacctgggcc gtgacaaaca atgtcccgga cacctacccc agcgaatggt
     2821 ccctggcgac cgcccgcatg gcgctggccg cggcggccta cgagtcggaa agctggctgg
     2881 acgccgcgga atactcggac ctggtgctgg aggccctggc ggacgtcacg gagcgcctgc
     2941 ccctgccgag gtactatgcg gtccgcctga tccccgaacg ccgggactgc ctgtggcgga
     3001 tcgctgagta ccccttcgtc tacggagacc cctggaagtg gcggctgctc tacgacgcca
     3061 acaagaaggt cttggtgaac cccaacgatc cggacctgat ccttcccggg caaattcttg
     3121 agattcccag catccgggga gagacccggt ccgggacctg ggatcctacc aagccctacg
     3181 tggaactgcc gaagccataa ctcttcttcc gatagggagt taagtattgc gaggccgccc
     3241 gtgggcggcc tcgcttttta atgggcgaaa gtccgatcag ccttccccgg ggatctccag
     3301 ccgcttcctc cggttttcca ggatggtccc ctccgcgcag gccgccatgt ccaggacgca
     3361 gggcgccacc aggcggtact ccaccagggt cccccggcgc tcgtcctcga cgaatcccgc
     3421 ctccttgagc tgggacaggt gcttggaggc cgcggacttc tcgaggccga tctccgccgc
     3481 gagctcgcag acgcaacggg gccgttcccg gagcttgtcc aggatgtaga ttcgcgcggg
     3541 gtgcgccagg gccttgaaca tctcggcccg cagggaatac ttgcgcggga tcgtgggttc
     3601 catgcctcaa tgttgcaaag tttcgaaaca attgtcaagg aacctcttga cagatttatg
     3661 caatgtttcc ataatttgca acatggagga accatgaaga tccagatcct ggggaagggc
     3721 tgccccaact gcgaactcct ggcccagcgc gcccgggaag ccgtcgcgga actcggcctg
     3781 gatgcccagg tcgagaagat cacggacgtc gacaagatcc tggacatggg ggtgatgatg
     3841 accccggccc tggcgatcga cggggacgtg aagcgggtgg gcaaggtgct cgcgaagagc
     3901 cagatcatcg agatcctgaa gggagggaac tgacatggcc ggatgcacct gcggagggac
     3961 gtgcgagacg aaccttctgt acgcctgctc gggggcggcc aacacgggtc tcctggcgga
     4021 ccaggtgatg cgggccctga tgcgggacaa ggtcggagtc ggcacctgcc tggccgccat
     4081 cggggcgggg ctctccggat acctgcaatc cgcccagtcg gcgacccgca acatcgtcct
     4141 ggacggctgc ccggtggcct gcggagccaa gatcttccag aaggccggca tccccttcca
     4201 gcaccacatc ctcacggagt acggagtgga gaagggcaag acccccatca cgggagatgt
     4261 gatcgaggac gtggcggccc gggtagcggc caaggtccgc gcatgagcgg gaaggcctgg
     4321 actccgaacc ggaagctcct gtacctggcg ggcgccttcc tggccgccta cttcatcccc
     4381 tggggcgccc cccgggtaac cagcgccgtc caggaagccc tcctcatgct ctccgagtac
     4441 gcccgggagc acgtcctgct ctgcctggtg cccgccctgt tcatcgccgg ggccatcacg
     4501 gtcttcctga accagcaggc ggtcctgcgc tacctggggc cggacgcgaa ccgggtgacc
     4561 gcctacgggg tggcttcggt gtccgggacc atcctggcgg tctgctcctg cacggtcctc
     4621 cccatcttca aggggatcta caagaagggt gccgggctcg gacccgcgac cgccttcctc
     4681 tactccgggc cggccatcaa catcctggcc atcgtcctgt ccgccaaggt cttcggatgg
     4741 aggctggggc tggcccgcgc cgtcggggcc gtgctcttct ccgtcctgat cggactggcc
     4801 atggccctga tcttccggaa ggaggacgag aggcgcgctg ccgacgcccg cctgttctcg
     4861 ggatcccagg agaagccttc ccgcggcctg ggacagatga tcgtctacat ggccagcatg
     4921 atcggcatcc tggtcttcgt gaactgggcc ggttcccggg gaggcagcgc cctctgggac
     4981 gcgatccact ccgcaaagtg gtggatctcc ggggccttcg ccctggtcct cgcctatacg
     5041 gtgatccggt ggttcgaccg ggacgaacgg atcgagtggg tggtcgccac ccgggacttc
     5101 gccctccaga tcctgcctct cctcttcggg ggggtcctgg tcgcggggtt cctcctgggc
     5161 cggccggggc gggacgccct gatcccgggc gcctggatcg ccggactcct gggcggcaac
     5221 tccctgctcg ccaacctctt cgcgtccgtc gcgggcgccc tcatgtactt cgccaccctg
     5281 acggagatcc ccatcgtcca ggggctcctg ggctccggca tggggcaggg ccccgccctg
     5341 gccctgctcc tggccggacc gagcctgtcc ctgccctcga tcctggtcat cgccggagaa
     5401 ctgggctgga agaagaccct gacgtacgtg ggattggtcg tcgccctttc gacgctggcg
     5461 gggctggcct tcggggccgc ggcctgaggt gtcgaccggt cctgcccggc ggtccgggcc
     5521 ggaccgccgg tcccgcgggt caactccgtt tcgatcgccc ggcggagcag cggacgccgg
     5581 ccccgggcgg ccggatctcg tccggggatc cgtggtcccg catcccttcc tcgatgcagt
     5641 cgaccaggtg gctcacgcag tcgcagacga tggagtaccg gctgaactgc ccctcccgca
     5701 cgtcccgcac caggcccacc cgtttcagga cggccaggtg cttggacacg gtggtgatgt
     5761 cggaaccgac ggcctccgtg agctccccga cgcagcgggg tgcctcctcc agcatctcga
     5821 tcatcaggag acgggaagga tgggccaggg acttgaggat ggacgccctg cgcagaagcc
     5881 gttcccgatc cggaatctcc atgttccatc accatcctga ttggcgattc ggccaaacaa
     5941 tgcttgacac atttggcctt tcggccaata ataaggcgag attcgtcgtc tgtcaacgct
     6001 tcaagccaaa ggagcatcca tgagcgacac gccgaacgca ggtctgggat tcttcgcgaa
     6061 atacctctcg ctgtgggtcg cgctctgcat agccctgggt gtggcgatcg gatacttcct
     6121 gcccggggtt cccgccttcc tgggcaggtt cgagtacgcc aaggtgtcca tacccgtagc
     6181 cgtcctgatc tggctcatga tctaccccat gatgttgaag gtggacttca ccagcatccg
     6241 aaacgccggc cggaaaccca aggggctcat agtcaccctg gtcgtcaact ggctcatcaa
     6301 gcccttcacc atgtacctga tagcctggtt cttcctcaag gtcgcgttca aagccttcat
     6361 accttccggc ctttcgacgg aatacctggc cggcgccgtg ctcctggggg ccgcgccctg
     6421 caccgccatg gtcttcgtat ggtcctacct ttccgacggg gatcccggct acacggtggt
     6481 tcaggtggcg gtgaacgacc tggtaatcct cttcgccttc acccccatcg tggccttcct
     6541 gctgggcgtg agcgacgtcc gggtccccct ggacaccctg atcctctcgg tcgtgctctt
     6601 cgtggtgatt cccctgggat tcggggccct gacccgctcc cggctcatcc ggaagcgcgg
     6661 caaggagtgg ttcgacgggg tgttcgtgaa gaagttcgac ggcatcaccg agggcggcct
     6721 attgctcacc ctagtcatcc tgttcgcgtt ccagggcgag gtcatcctcc gaaatcccat
     6781 cgccatcctc ctgatcgcgg tccctctggt tctccagacc tacttcatct tcttcatcgg
     6841 gtacggatgg gccaaggcct ggaaggtccc ctacgagata gccgctcccg cggggatgat
     6901 cggggcgtcc aacttcttcg agctggccgt ggccgtggcc gtgagcctct tcggcctctc
     6961 ctcgggcgcc gctctggcca cggtggtggg cgtgctggtg gaggtgcccg tgatgctgtc
     7021 cctggtccgg atcgcccggg cgacccgttc ccgattcccg ggcgcggcgg agccggaagc
     7081 cgagctcgtg ccctcccgcg cgaagtagcc ggcggtcccg cgaaggaggc gttcgatgtc
     7141 cagccgggcc cgcgtcgcga cggatttttc aagcgtcacg ggcgcccttc cccggacgga
     7201 agggatcctc aacctcaccc ccgcggaggc cgccgagtac ctctccaagg gggcgatcct
     7261 ggtggacctc cgggaggcct acgagacgaa tttccgggta ttcgaggttc cccagaccat
     7321 ccacctcccc tggacccttt tctccaaggg cttcgagcgg ctgccgcgag accttcccct
     7381 ggtcctggcc gacgcctcgg gcatctacgg cagggaggcc gcccggatgc tccggaacgc
     7441 cgggttcacc aacatcgcga agatggcggg cggcatgatc gactgggacc gggagggcct
     7501 cccggtgagg agggatcccc tgttcgagct atccggacaa tgcgcgtgca agctgaaaac
     7561 ccgtacgggc aagaaccccc tcctggacaa gaacgccgtc gtgatcgacc cgtcccggga
     7621 ggagtcgccg tgaagaaggt ccttttcctg tgcgtccaca actccgcccg cagccagatg
     7681 ggagaggcct tcctcaaacg cctcgcccct gaacggtttg aggccgagag cgcggggctc
     7741 gaaccgggga cgctcaaccc ttacgtggtc cgggccatgg cggaggccgg aatcgacatc
     7801 tcccacaatc ccaccaagag cgtcttcgac ctgcacgccc ggggcgcggc gttcgactac
     7861 gtgatcaccg tctgttccaa ggaggcggcc gaacgctgcc cgatcttccc gggccgggcc
     7921 gagcggatcc actggccctt cgaggatccg tcctccttcc ggggaagcga cgaggagatc
     7981 ctggcccggg tccgctcggt gcgggacgag atcgagcggg ccgtgcggcg gttcatctca
     8041 gagaggggct gacgatccga cgggaagccg cgggacccag aatcgcgagc cgttccaccg
     8101 tttgcggttt ctaaccggat ccgtcatact ctccttggag gggaacggat gctcgacacg
     8161 ggccgcctgg ccgcggatga acgccgcgcc tacaggatct tcctcgtctc cctgctgggc
     8221 gccctggtgg tcctgttcac gggggtgttc ctgggcttca ccgcgcgcaa cgcggacttt
     8281 ctccgggaag tcgtgctgga gcgggcccag tccctgttcc ggcagatcgt gatgacccgt
     8341 cagtgggcgt ccgagtacgg cggggtctat gtccggaagg gcccgggggt ggaatccaac
     8401 ccgtggctgg agcaccccga cctacaggcc gcggacggtt ccctcctgac cctgcgcaac
     8461 cccgccctga tcacccggga gatttcggag atcgcctcgg tgcgggacga ctaccggttc
     8521 aagatcacca gcctcaaccc cctgaatccg gggaatattc cggacgactt cgagcgaagg
     8581 gccctgcgtt ccttcgaggc cggcgccctg gagtactggg aaaccacgga cggccaagcg
     8641 ggccgggagt tccgctacat gggggcgctg aaaaccgagc gatcctgcct ggcctgccac
     8701 gccaagcagg ggtaccgaga gggggacatc cggggcggca tctccgtcac cttcccggtg
     8761 gaccggctcg agggggagct gaagaagaac gtcgccctgg tcttctggct cgagcttctg
     8821 atcgcggcgg ggaccctggc ggccgtcctc ttcttcgtga tccgcctccg gcggcagttg
     8881 gaccgggtgc gttcggaact ggagcaggcc gccacccggg acgccctgac ggggctgtac
     8941 aaccgacggt acatcctgga gcgcttcgtc caggaggaat ccaagtgccg gaggtccggc
     9001 cagcccctgg cctgcgctct gatcgacgcg gacgacttca aggccgtcaa tgaccggttc
     9061 ggtcacgccg cgggggacgc tgccctgcgg atcatcgcgg aaaacttgaa gtccggggtc
     9121 cgggaatacg acatccccgg tcgcttcggc ggcgaagagt tcatcatcct attcccgggc
     9181 gtacgcgccg cggaagcccg gatcgcctgc gaccggatcc gggcggccat cgaggcgtcg
     9241 ccccggcccc cggtcctgga aaccgcccgg ctcacggtca gcgtcggaat ctcggatacc
     9301 gtctgctccc ggggcgccgg gacgaaccgg gacagcctgg attccctgct gttccgggcg
     9361 gacgaggccc tctatcgggc caaatcgggc ggcaagaacc gctgcgagac ctgcccggac
     9421 gaatccggaa gcccggaccg ggaacccggg acttgacccg gattccctag gagcctgtcg
     9481 gaagcggcgt cctcagggcg tccggggcgc gaaaacgatt ctccggtagc ctcgccgaag
     9541 cccggatggg cttcggcgcc ggcaaggggc gcgaaagcga tcctccggaa acatcgccga
     9601 agccccgaag gcttcggcgc cggcaagggg cgcgaaagcg attctccgga aacatcgccg
     9661 aagcctcgca ggcttcggcg ccggcaaggg gcgcgaaagc gccccttgcc gctattccag
     9721 gcccaggatc ggcatgcccc tcgggtactc cacgctgaag gaaaggggca ggaccgactc
     9781 ctttccggcg gggagcttca gggtccacac gaacgtctcg ttctcgatct tgcggagggt
     9841 gtccgtatcc ttcgaatacg gcggagccag ggccttgacc acgatctgct cgttcaggga
     9901 caccgggagc tggtcggaga cctccaggac gatgtcccgc ttcttggagt tcttgacctt
     9961 gatctcgtac tcccaggtgg tcttggattt cttggtcagg gcgccggtgt tctcgtcgaa
    10021 cttgcgcagg agcttccgct ccacccggat gccctcgtcg atccccaggt cggccttgaa
    10081 ggaaccgccc gcgggcacgg cttccagggc ggcgtcggcc acgtaggacc cgtccacgta
    10141 gacgtgggaa ggcccggcca gtatgggata ggcggaatcg ttcttcactt cgcagcggaa
    10201 gtaggcgtac ggcgagagct tgggtatcgc cgcgtaggag aaggtcacgg gcaggtccag
    10261 gacggcgatg gtgacagtcc ggtccttgtt gtccgaggac accgtggtgg cccccgggat
    10321 ggcgaaggtc acggcggtgg cgcccgtgga cgcttgggcc gtgtcgatcc gcatctccgg
    10381 ggcgggttcg ccccacgcta ggtccgaaga ggattccgcc gcgccgcgga cgcccgcggc
    10441 ggccggagcc ggggccgact tggcggcctc gtcccgtcgc gctgagggcg ggctgtagat
    10501 atccaggtac cagggcggca ggaccgggag cgctcccccc gcctggggcc gggctgtgga
    10561 caggaacagc tccgcgccct cccagccctc cccggtgttc tgccgaacca gggcccggta
    10621 gtggacggag aggcgggcgg cctcggaatc ggcgcgaagg acgtagtcgg ggcgccagga
    10681 agggccggcg accaggtagg agacttccag gaccgccttg gtcggagagg aggcgtccag
    10741 gaccagctcc gcctcggtga ccgagaggcg cgtcccggcc cccaggctcc ggatctcccg
    10801 gttcacccgg tcgatctccg cctgcagggc ctggatctcc cggcgggagg tccggaccgt
    10861 ctcgttcacc gccgcgttcc gctgtcggtg gaaatccagc atcttggccc aggagccggg
    10921 atccaggggc agggattcgg aatttccggc gttggaggtc aggcgcttgg ccatgtccgc
    10981 caggaaagtc ctctccgcct cggcctcccg gacccggtcg ttgaccaggg tcagcttgtc
    11041 ctccaggccg cgcttctcgg tttccagggc cgtgagctgg gcggacacgt cccgggtaag
    11101 ctggcgggcg gccaccctca cgtcccgcag ggtgaaggcc cccttgccgg agacctggac
    11161 gctggcgggg tccaccgagg cgggcaggtt cccgaagagg accgtggact cgcccttgaa
    11221 caggctcacc tcggcccggc gggtcaccag ggcccgatcc ccgtagaccg tcacccgcac
    11281 cacccgggaa accgcggtct cctgggcgcc cagggccgcg gacagggcca gaaccgcgaa
    11341 cagggtcatg acaagtcgct tacgcatagg tcctcctccc ggatggatgc cgaatcaaga
    11401 ctgcccggag ccctcaggcc ccggggcctt tctcgtaaaa gcggcgcaag gccgcggtca
    11461 tgaaatcgtg gtcgtaggat ccggcggtct cccggatgga gtgcatggcc aggagcgggg
    11521 agcccacgtc cacggtccgg atcccgctga gggcggagga cagggggccg atggtcgagc
    11581 ccggggtcag gtccgcccgc acggcgaact tctggcaggg gaccgccgcg tcctcgcagc
    11641 aggcgcgaaa tcgggcctcc gactcggcgt ccgtggcgta cttgaagttg gcgttggcct
    11701 tgaccgcggg tcccccgttc aggacggggg agtaggcctc gtcgtacttt tcctggaagt
    11761 tgggatgcca gccctgggcg gcgtccacgg acacgcagaa cgaggccgcc agggcgcggt
    11821 agaagccctc ggaatcgcag cccgagcggg cgcagacccg ggccaggaca tcccggagga
    11881 agagtccgtc cgccccctgc cgggtcccgg agccgatctc ctcgttgtcg aagaagacgg
    11941 cggcctggcc gtgggccccc gggggggccg cgttgaaggc cgccaggatg gcgtgacagc
    12001 ccgccaggtt gtcgatccgc cccgagttga ccagctcccc gtcccgaccc aggatcaccg
    12061 cccggctcgc atccaccagg aagagctccg cccccaggat gtcccgtacg ggcacgccca
    12121 ggttccgggc cgtctcggcc agggcccacg gagcgtcccc ttccgccggc ctccggtccg
    12181 ccaggggggc ggccaccagg gcgggcaggt ggttctgcac gttgtactcg aaccccttgt
    12241 tgacctcccg attgaggtgg atggccaggt tcgggatgac cgccgcgggt cccgcggggt
    12301 tgaccagccg gagctccggg cggccgtccg agccccggac cgcggcccgg cccgccaggg
    12361 ccaggggccg gtccagccag gtggacagga tgggcccccc gtagacctcc accgcgatcc
    12421 ggtcccccgc cctgtgccgg agggactttt ccggccggac ccttagggcc ggggcgtccg
    12481 tgtgggcccc cgccagggcg aacccggtcc gggcggggga ttcgccgccg atccggaagg
    12541 cgacgaggcc ggagccgttg cggaccacgt agtaggtcct gccgggttcc aggttccact
    12601 cgctccgctc gtccagggcc agggctcccc gggcctggag ggccgcggcc agggcgtcgg
    12661 cggcgtggaa ctgggtgggc gaggcgtcca gaaaatcgca gaggctcttc gcggaaacgg
    12721 cgggcatggc ggctcctgac atcgggttct tccggaaagg gtatcacccc cggggcggtc
    12781 tttcaaggaa ggctgttcag ggaaaggatg taggcccgga ccaggagggc gcggatctcg
    12841 aaggcccggg cccggaggtc ctgccggggt ccggaggcct cccggatcag cacctcccgg
    12901 gcggcctcgg gggagagccc cctccgccgg accaggtgct tgacccggag cagggccagg
    12961 agctccgcct cggagtattc ccggcggccg gactcgctgc gccgcgggga gaccaggggg
    13021 acggaccgct cccagtaccg gatcacgtgg gccttctccc ccaggacccg ctccacctcc
    13081 ccgatcgagt aggagcgcac ggccctacgc ccggtccgcc ttccaggagg aggaaccccg
    13141 gacttccagg cccacgggga tcagggagaa ccccaggtcg gagcgtatct tgttcttgag
    13201 gtacgagagg taggactccc cgacggcgtc ggggcggctt gtgaagagga tgaacttgac
    13261 cgggttgatc ccggactgca ccgcgtagcg gagcttgaac cgggtgcggg gtcccacggg
    13321 gggcggatag gccgccaccc agtcctccac ggccttgttc agggcggagg tctcgatctt
    13381 ccgtgtgagc tggccgaaga ccgccaggag catgtccagg agcttgggga ctcccgttcc
    13441 ctccttggcg gacaggggaa ggaccggggc gaaggccatc tggccgaaga agtaccggag
    13501 cttgtcccgg gaggcctcga aggagttctt caggtccggc atccggtccc acttgttcag
    13561 ggcgaagacc agacccctcc ccttctccac cgccagggcg gcgatcttct tgtcctggtc
    13621 cgtgagaccc tcctgggcgt cgatcatgag gaccacgata tcgcagtcct ccacggtgcg
    13681 gatggcccgg ttcacggagt agtactcgac gtcctcgtgg actttcttct tccggcggat
    13741 ccccgcggtg tccaggaccg tgatgcgccg tcccttccag gtcgtggatc cctccaccac
    13801 gtcccgggtg gtgcccgcga tctcgctgac cagggacttc tgttccccaa gaaggcggtt
    13861 caggagggtt gatttgcccg tattgggttt gcccaggatg gccacccgga cgtcgtcgtg
    13921 caggtcctcc tccgaggaga ccttggagaa gtccaggcgg gcgacgatgc gctccaccag
    13981 ttcgtccacg ttccggccgt gctccgcgga cacggccacc agctccgaaa agcccagccg
    14041 ggcgaagccg taggccaggg cgtcccgctc cgggctgtcc gtcttgttga ccaccaggat
    14101 ggtgcggtcc gcccttcggc ggagccgggc cgcgagctcc tcgtcctccg gggtcaggcc
    14161 ggtggcgtcc accaggaaga ggatgaggtc ggccttgtcc agggtggcca cggaccgccg
    14221 ggacaccagg tcgttcatgt ccgatcgctc cagggtcacg cccccggtgt ccaccaggag
    14281 gacccggcgc tccgtgcccg gcaggacgca ttcggcgtcc acgggatccc gggtgactcc
    14341 cggggtgggg tccgtgatgg ccctgcgctt gcgcaggagt cggttgaaga gggtggattt
    14401 gcctacgttc ggccgtccgg ctacgaccac gacggggagg ttccggtatc gggtccgggc
    14461 cgcgggtgcc gcgggggcgg cgggttcctg ggggttcgtc tctcgcatgt cttccttgcc
    14521 gctcggggcg gatctgtttc ggccggtcgg gccgttcggg ttcaccggaa tacgcccgcc
    14581 tccgggtcga tgcgctcccg gaggtagcgc tcgatctccg cggcggactc catggccagg
    14641 acctcccggg ccagggaggc ggcctcgccg gaatccgcgc cccggaggag gcgcttgatt
    14701 tccgggatgc tgacgctgga catggagaac tcgtccaggc ccaggcccag gaggaccagg
    14761 gccgcgtagg ggtccgaggc catctctccg cacattccca cggaaatgcc cgccgcccgg
    14821 gccgcctcga tggtggcctt gatcaggcgc aggaccgcgg ggtggaaggg ttgatggagg
    14881 taggccacct tctcgttgcc ccggtccacg gccagggtgt actggatcag gtcgttcgtc
    14941 ccgatggaga agaaggccga cttcttggcc aggatgtccg ccaccagggc cgcggagggc
    15001 acctcgatca tgatgcccac ggggatgttc tcgtcgaagg cgaagcccgc cgcccggcat
    15061 tctgccttgg cgcgttccag cagggccagg gcctggtcca gctcctcggg cacggagatc
    15121 atggggaaca tgatccgggc gtccccctcc acggaggcct tgaggatggc ccggagctgg
    15181 gtcaggaaga ggtcgtcccg ggagaggcac aggcggatgg ccctccatcc caggagggga
    15241 ttcttctcct cccggcccga gagctcgggg agggccttgt cgcctcccag gtccagggtc
    15301 cggatggtga cgggtcggcc ttccatggac ttgagcacgg accggtaggc ccggtactgc
    15361 tcctcctccg aggggatgcg gccgggctgg aggaagagga actcggaccg gaacaggccc
    15421 accccgtcgg caccgtgcct gcggaccgtg tccacctcct cgggtatctc gatgttggcc
    15481 ttgaccagga tggaccggcc gtccttggtc cggacgggca ggacctcggt agccaggagg
    15541 gccttctccc gctcgacgtc ccgagatcgg acctgctcgt agcggatcag ggtggattcg
    15601 tcgggttcca cgaccaccct gcccttgcgg ccgtccacga tcaccccgtc cccgttgcgc
    15661 acgttccggg tggcccccgc gacgcccagg accgcgggga tctcgaaggc ccgggccagg
    15721 atggccgcgt ggctggtctt cccgcccccg tccaggacca gggctttcac cgcccgcttg
    15781 ttcatggcga tgatgtccga gggcaggagg ttgtgggcga ccaggacgat ctcgtcctcc
    15841 aggtcggaga gggtgaaccg ctcccggaac atcaggtggt tcaggatgcg cttggtgacg
    15901 tcccggatgt ccgccacccg gtcctggatg tagggatcct ggacccggga gagctgctcg
    15961 atcagttcct gctcccgctg gaagacgatc cactcgatgt tccggaggga ctcccggagg
    16021 gaggactcga ggccctccat gagctcggga tcctccagca tgaggaggtg ggcgtcgaag
    16081 atcgcggaat gggtcggacc catctcgctt cgggcccggt cccggagctc cgtgatctcc
    16141 ccgcgggcct tgccgatggc cgtccggaac cggtcccact cggaatccac gtccgccgtg
    16201 cggatatagt agttcggtat ggagacgtcc tcgtcctcca ggaagaggaa ggcttttccg
    16261 atggcgatgc ccggggaaac cgggaccccg gtcaattcta tcattggacg tattttagcg
    16321 tagaccgggt ggggtgtcaa accggagggg cggacgttcc gccggggcgg atcagttcag
    16381 caggagcatg gggttgaccg tccggccgtt cttgaggacg gtgaaatgca ggtggctccc
    16441 cgtggagtac cccgtgttgc ccaccgcccc gatccgtgtc ccgatggaga ccgcctggcc
    16501 tttccggacc gctatgctgt ccagatgtgc gtacatggac gcgtaccccg agtggtggga
    16561 gatgatgacg tagttgcccg agacggcgtt gtaccccgtg gtggataccc gcccctccat
    16621 ggccgcccgg accgaagccc cgtggggggc gccgatgtcg atgcccgtat ggaaggtccg
    16681 ggtgccggag aagggatcgt ccctccatcc gtaccagctg gtgatccagc cccggatcgg
    16741 ccacttgaac aggtccccgt tgatctcccg gagggtgaag ctggaaagcc gggcgtccgg
    16801 gaggaagagc ttccggccct caaaaagcgt accgggaccc acccggttca cctcggccac
    16861 cctgtccgcg gggacggaat acttggccgc gatcgattcc accgtatccc ccgccgtcac
    16921 ttcgtgcagg atgccgttca tgttgggaat cttgaggtgg gtcccgatcc ggatggcccg
    16981 ggtattccgg atgtcgttga agctgatcag ggtgtccacg gtcacgccga aggcctccgc
    17041 gatcattccg ggtatatcgc ccttcttgac ctcgtagacc tggtaccaga gcccctcgtc
    17101 ccggggcggg gtctccgcga taagttccgc ccccgcctcg gcctcgaggg tctccaaggc
    17161 gagggtccgc atgtccgcgg agccgacgga cacggccgcg tcggggaggc ttgccgggga
    17221 acccaggaag agcgccgcga agacgagcgc cgaggatacg gccgcgaagg tcccgcgccc
    17281 gtcgaacgcc tcaagggggt ccctatgaaa aaaccttgca gccctcatcc cctgccctcg
    17341 cccgcaccgt cagactctcg aaccgctcgt tccacggtcc atcctaaacg aaatactccg
    17401 gccccgcaac cggaaatatg tggctccgcc tcccggcccc tcaatggggc aggatgatga
    17461 cgctcacctc tatgccccgg tccagggccg cggcctgggc ggcggccttg tcgatcttac
    17521 cccggggcag gggatggggc ggggcgtccc cgatcagcac gatcatccgg gcctccgcgg
    17581 accagggaaa ggccgtggcc gcctcgtgca gggcctcgta caccgcttcg gggatgtcgc
    17641 ggccccccgc cacccggacg gcggtcacgt tggccgtgaa ggccccgaag tccgcggtga
    17701 aatcgtatcg cttggtcagg tagtcctcga agtagtcctt gtagaggacc aggcccagcc
    17761 ggaaggagcg gaactccgag atccggtccc ggatcatggc cggaaggctc tccttcaccg
    17821 cgtcgatgtc gtccttcatg ctgtccgtgg tgtccaggca gagcaccagg tccacggact
    17881 tcccccgggc ggtgtccagg atcttcccga gcttgtcgtt gatgtcctcc cggcccgtcg
    17941 aaaagagggc gtcgcccttg cccgcctggg cgatggcctc gaaggcccgg acggtctcct
    18001 tcatgtacag gctcttgtcc ggctccgccg ggggcgtcgg gggaggcgcg ggccggggga
    18061 agggcttctg ggtcacccgg atctcgtagg gattgtcccg gaagcccccg gtatagtccg
    18121 cgtagggctt ggggaaggcc cggatgttca cgaaggtccc gtccgagagg aagacctccc
    18181 cctgtcgcga ccaggggtac ccgtactcta cgacccaggg caggaatatg tggaaggcgg
    18241 atccgagttc cggatccggt tccggggtgg aatcgatcag ggaccagatc ccccgggcgg
    18301 gatccaggaa cttgccgtcc agcaggcgct tctcgtcccc gttgacggga ttcttctccg
    18361 gggcccggta ggcgtaattg tccgccttac ccgcgggatc cttggtggtc tccgtgagca
    18421 ggacgctccc caggccgggc ttggcgcgga tgtagaggtg atagcccccg tcggccctct
    18481 gctcgatccg gagatcctcg ggggcgaggg tcagggcgga cgcctccccc ggtgtctggg
    18541 cgcccgcgga gagggcgagg gcggccaggg cgatccacag gacttcgcgg gtcttcaccc
    18601 ttccagtatc ggcgcggatc ttccttcgaa aaagcatgga ggaagctgga aaggagcgac
    18661 aggataacag gatttttaaa ggatcaacaa gatagatcct atatttttta catatataga
    18721 attggtttaa aaatacgata gccagcatcc aactgaattc atcatagtga acgagaaaga
    18781 tccggaaaaa caggatccta tatcgcgttc catgtgagat catggacgac atttccatcc
    18841 tgtccatccg tattcaatcc tgtgtatcct gtccgctccc gtccccgtat tcgtccctag
    18901 tcaattcctt ttaggagccc gaaacggagc ttctgggtca ggatccgggc cgcggcgtcc
    18961 cggacccggg cctcccgaag ctccccgctc cggaccgcct ccaggagggc ccgatagacc
    19021 tcccgggcga cccgtccccc gcttgccatg accatgtcgg ctccggcccg gacggcggca
    19081 accgcggcgt cccccgcacc gccctccccg gacagggcct tcatggccac gtcgtcggtc
    19141 aggacgacgc cccggaaccc gagcccctcc ttgagccgcc cgatcaccgc cggcgacagg
    19201 gacgccgggg cctccgggtc cagggccggg aaccgggcgt gggaaagcat gacgaccgcg
    19261 acgtcggccc ggatcgcccg gcggaagggg ggatcgtaga acgactcgac ctccccgagg
    19321 gccgcgggaa cgaccgg
//
LOCUS       DAOTEI010000051        19296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1872_length_19296_cov_5.923912, whole genome shotgun sequence.
ACCESSION   DAOTEI010000051 DAOTEI010000000
VERSION     DAOTEI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 19296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19296
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1872_length_19296_cov_5.923912"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="P5313_06865"
     CDS             <1..603
                     /locus_tag="P5313_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY80118.1"
                     /translation="EMLCTWARDHGVVGTGVDLCRSFSEQARSRAGELGVAGRVRFIH
                     GDAAGYVSDEKVDVAACVGATWIGGGVAGTVELLSRSLRPGGILLIGEPYWRNLPPTE
                     EVAKGCLAGSISDFLPLPELLASFGRLGCDVVEMVLADQDGWDRYEAAKWFTMRRWLE
                     ANPGDELAEEVRARLSSEPERHARYTRVYLGWGVFALMPR"
     gene            707..1120
                     /locus_tag="P5313_06870"
     CDS             707..1120
                     /locus_tag="P5313_06870"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY80119.1"
                     /translation="MKGIPYVNFNGNCEEAVRFYHSILGGELELLRFKDIPEGGGMRP
                     GGTWNDKIMHGSLKFKGGNYLYFSDSWEENPVTVGSNVTVHLEVDSEKDVYTFVEKLG
                     AGGTIQMPAAKVFWGSVYGSVVDRYGVCWGIEYQL"
     gene            1167..1658
                     /locus_tag="P5313_06875"
     CDS             1167..1658
                     /locus_tag="P5313_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY80120.1"
                     /translation="MAGKPVPCLWFNNTAKEAVEYYLSIFKGGRILSIDHYTEVGEEI
                     TGHKKGDIVTIEFEILETRFIALNAGPEFSFNPSVSFTIECSDQEEIDYYWERLSSKP
                     EAEQCGWLQDRYGVSWQIVPRMLSEALLNGTPEQRRRLTEVYMDMKKFDLEKLEAALR
                     TEP"
     gene            1655..2029
                     /locus_tag="P5313_06880"
     CDS             1655..2029
                     /locus_tag="P5313_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRY80121.1"
                     /translation="MSGTPKGGTQAEKFRRMVFAKVYPLYLEKAAKKGRSKEEVDMVI
                     SWLTGYDEQGLRKRIEEGCDFETFFDGAPRFNPDAVKITGLICGWRVEEIEDPLVRKV
                     RYLDKLVDELARGKAMDKILRK"
     gene            2093..3763
                     /locus_tag="P5313_06885"
     CDS             2093..3763
                     /locus_tag="P5313_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRY80122.1"
                     /translation="MSASLPICRVPESKTAVSLDLSMAARHGLVTGATGTGKTVTLQA
                     LAQGFSEAGVSVFCCDVKGDLSGIAAPGGNNAKIGAAFGKLGLPEPAWKGCPAAFWDV
                     FGKKGHPLRATVSDLGPLLLGRLMGLSDVQADSLRLLFRIADEKGLPVLDLADLRALI
                     GWATDNAAALKAEYGLLSSASLGALQRSVLVLEDSGAGRFFGEPALQIGDLIRTAPDG
                     RGMVHVLASEDLMAEPTLYATVLLHILGELFEELPEAGDLSKPRLILLLDEAHLLFDD
                     APKALVDAVERTARLVRSKGVGLWLVTQSPLDVPESVLGQLGNRVHHALRAYTPKDRK
                     AVDSVANSLRPNPRVDAAAAITELGVGEALVSFLDADGRPTPVERARVVPPASRIGPL
                     SPEERSALVAASPLGTRYDKPVDRESAYEMLRGRMASTGSGTSGGQGAGTRSPGKAGP
                     GAGSREPTIEEIEAELRGWPMPSRTEPEYPDYAEEPPARRAPVSRSSGGSRPAARSGS
                     RSVRSGGYAASGGSFAEDLARLALSAAGRELGRQVVRGLLGTPSRRRR"
     gene            3894..4760
                     /locus_tag="P5313_06890"
     CDS             3894..4760
                     /locus_tag="P5313_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY80123.1"
                     /translation="MNVTVWGCRGSITSPGRDTLRYGGETTCLEIVTDGGDRIVIDAG
                     SGLRRLGQHILKDPDVADITLLLTHAHWDHLVGFPFFRPAFFSKYTITLCGGSDPRIS
                     VLKSLRHQMEPPYFPVDMSVMKAKFVTGCTCGCSECGNELAVGSGSHRCESIPLSHPN
                     GGYGFKLTDNRGRAFVLLTDNELRYRHETGLDREGYVEFCRDARLLFHDACYTEEKYE
                     RTRTWGHSTYKDAIDLAIDAGVERLGLFHHNPDRGDDEIDGILGWCRSYISGRGSGLD
                     CFACAEGMTLAV"
     gene            4867..5661
                     /locus_tag="P5313_06895"
     CDS             4867..5661
                     /locus_tag="P5313_06895"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HRY80124.1"
                     /translation="MIFIPAGLAALAVLFFFLGRGQGRKAYDMQTTETSSAADLARLA
                     SDIAQEIGAGSLAQTAEIKGTIECDAPLSAEMSGTGCVWYRSVVTREYEEPYTERDSD
                     GKTRSGTRRGSETVSSNERRVPFRVRDASGSCEVDPEGAAMDGERVLSRFEQGDQGPS
                     LSFGGLTVRLGGIGAGRRTIGYRLEEWAVPTGRAVYVLGEARDDGGRLRVGKPSAKGG
                     RFLVSLKTEEELVRAAKSGSTILTVLAGLFTAAAVVVLVLVLTGVI"
     gene            complement(5856..6254)
                     /locus_tag="P5313_06900"
     CDS             complement(5856..6254)
                     /locus_tag="P5313_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY80125.1"
                     /translation="MRFNDSSPIYEQIADDLAAQAVSGALTAGERLPSARDLAVSLQV
                     NPNTAARALAALADRGVARCERGTGYFLAEDGPAVAARERRDRFFSEELPKLFVRMEE
                     LGIGMETIQEAWRSRTAGTGGSKAAEERTI"
     gene            complement(6241..7038)
                     /locus_tag="P5313_06905"
     CDS             complement(6241..7038)
                     /locus_tag="P5313_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80126.1"
                     /translation="MNDTTVRTPFSPERVALLARNRLLEDLSPFLIALGALAGLNVLS
                     ILLGGAAFLNHPSRHSGESGWAFLIWLGGVLLAGRAFSQMHNGRGGSDWILLPATSFE
                     KYAAAFVSYVVVYPLAAALAASGLSAILEGLAALLKASPGVVFNPLAGLDEGSVFGYL
                     FFVTASLAASARFGKLSLVKAGALFSAWAAFLGFLFVLGMLLSTPEGREALGNRHFSY
                     RFRPDFDPASEPVLIWVFRAWGWFSWAAAAVYGYFRVAEKEAVDEVQ"
     gene            complement(7035..7898)
                     /locus_tag="P5313_06910"
     CDS             complement(7035..7898)
                     /locus_tag="P5313_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80127.1"
                     /translation="MVRFDAVTFAYGKRPLFEGLELAMEAGSTVGLLGLNGAGKTSLL
                     KLGAGALFPASGLVEVFGKRPGSRSAAVLADVCFVPEDPWGPALSPETWLERYGAFRP
                     AMDAGLFRSLLEEFAVDPGKSVAKMSYGQRKKFAVAAALAGGSRAVFLDEPTNGLDIP
                     SKAQFRRALAAASTPDRVIVVSTHQARDLENLLDPVVILHGGKILCSFPASALSDRLS
                     VERLPGLEGRPVIYAERDALGWTALLAKPGSCADLELAFTAAVSRPEAFRAALEGRNP
                     GPYNPDAGDLK"
     gene            8081..9259
                     /locus_tag="P5313_06915"
     CDS             8081..9259
                     /locus_tag="P5313_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY80128.1"
                     /translation="MFDPPIPGRHDVRICVVGGGGSGAALAYDLALRGFSVLLLEKGE
                     FTSGTTGRHHGQLHSGARYAVGDRAIARECMEETLVLRRIVPEAIEYNGGIFAAIRDD
                     EADYAPAFVSACLESGIPAREIPVTEARALEPVLNPALKRAVWVPDGTLDAYRLAASF
                     LAAAAALGADLRAFTELVGIEVSDGRIRGGTVRGPDAREERVSFDVLVSATGAWAGRV
                     GSLAGLDIPVTPAPGTMVAVRGRLSDLVLSRLAPPGDGDILVPQRGLSIIGSTQSRAD
                     SPEGILPTRAEIEFLRVRADELAPGFSRAPFHAAWAAARPLAGRAASDAEGRGISRDF
                     AVLDHGAEGVSGMFTLIGGKATVLRAMARKAADAVCGFLDVEEPCRTADYPLPSWRAL
                     "
     gene            9256..10050
                     /locus_tag="P5313_06920"
     CDS             9256..10050
                     /locus_tag="P5313_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY80129.1"
                     /translation="MIEVNFRIRDGKDFVELSGGFPEDATVLDALETLRARAEGAAPA
                     ARVPHYRHSCHHGSCGTCGAVVNGLPSLMCLSRLSDLAAPRPREPGGPAVEPSHGPSG
                     ELVVTLEPLTRGSLVAGIAARPTSALEGIPEEYSYLRPSEDAGRASLPADPTRDGEKL
                     PALDGAVSPPLSGRVRFEACIECGLCSAACPVTAPFLGPAALAAVNRHREKAPESAES
                     MLALAAGADGAPACERHLHCSRVCPQAVYPGKHIQLLRKALAGRAQ"
     gene            complement(10044..10646)
                     /locus_tag="P5313_06925"
     CDS             complement(10044..10646)
                     /locus_tag="P5313_06925"
                     /inference="COORDINATES: protein motif:HMM:NF020136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HRY80130.1"
                     /translation="MKPLSFQSMFACLSLAALFAPRAPAEGAPMTPLLLDDFRTGISS
                     LGTRWEGFSDRVMGGISDLRLGIEGDAEGPYLALTGEVRLENRGGFIQARLLLKEGRS
                     SHYDGSKHQGIRLIARGAGDGYYIFLRTTSMVFPWAFYSAPLPVTEGWSEIRIPWSAF
                     VKGDFGAAFPLDVRKLRSLAVTAYKKAFRARLHVREIGFY"
     gene            10763..11950
                     /locus_tag="P5313_06930"
     CDS             10763..11950
                     /locus_tag="P5313_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80131.1"
                     /translation="MKFAPALLAALALASCAAAPATPPADSAPATPAAPVLSAGDEAA
                     IRAAVALRARAVAEGDKALYLSLLAPGDPAFLLEQSRWFDYRLSAVLSEFILEVESLE
                     ASGPGECAVALRQRYLYGPNGEVRDCRFVQLYRKVDETWLDADIAFRTLQSGHMEVRY
                     DEGVDGLRAARILGDAEKAWTTVRDSYGEAPARRTVVKLFSTQELLRQNTKITIGRLF
                     YGWGEPGESIKLFVRPDPSRSYVPVLAHELVHVVTLETSKNLCSWFAEGLANRFGNFA
                     VLGGTYLDSGYHKPSEYDRSVRWLGSVDSQTIDDEYEWGIYGGMSGAVVGFLAERYGP
                     DAPRAVVRALAAYPQVQEGYVYAEHDARFRGYLDGAMKTALGVGLEEFDLAWREWIRN
                     RVK"
     gene            12000..12686
                     /locus_tag="P5313_06935"
     CDS             12000..12686
                     /locus_tag="P5313_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY80132.1"
                     /translation="MPTMNEIYAAHADRYDELVSREDREGSLGRELGRLVPAGAAVLE
                     LGAGTGRVTRLYAGRVRSALLCDRSAHMLGRARSNLADFRDRLEFRVLDIREAGTLEG
                     GFDVILEGWALGHCALEEYERLSDFLSGTLAALDALLAPGGRIVLVETLGTNADRPAP
                     PGERLARLYDLLERDHGFRRTVIRTDYEFPTVEEARRVLGFFFGPDREGRISDRIVPE
                     WTGIWVRERG"
     gene            12697..13350
                     /locus_tag="P5313_06940"
     CDS             12697..13350
                     /locus_tag="P5313_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY80133.1"
                     /translation="MRIARKDGTFITTEFHGNGSPVLGVVVSGMGYTYRNPLLYYAVS
                     LLAESGADCLLVDLRYYEDEGFRALDEAGKDARFEADAAAVADAVEREASRRERIVLA
                     GKSLGTSVIRRCLRRPSVAEKSSLILLTPGSEWSAFIPELVRSGRPALVVGSFGDRYY
                     PVPNLDELRAAPRVRRLELEAGDHSLETGDAVRDVETLGRVVQVMKEFLTETAGPAR"
     gene            13551..14846
                     /locus_tag="P5313_06945"
     CDS             13551..14846
                     /locus_tag="P5313_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY80134.1"
                     /translation="MGDTRVAGTPARGLAGATLGFFIGFAAVSLFGPTVRYLQEAAGF
                     GPVQAGLLVSIPSLTGSLLRIPFSALVDTTGGRKPFLVLLGLSAAGLAGLWFLLSGDP
                     AGLPGRFPGLLVFGALGGCGIATFSVGISQTSYWYPQRMQGAALGVYAGLGNLAPGIF
                     AFLLSALSLPLLGLSGSYLAWLGILAAGTAGYALLGRNAPYFQLRSSGLGPEEAARRA
                     REDHGQELFPRGSVRESLAASASEWRTWALTGIYFITFGGFLALTAWLPKYWGSYFGL
                     SLGAAGSLAALFSVSASLFRAATGGVSDRLGGARTLALALGVIVLGSAGLALSGSAVL
                     SAAASLVLALGMGAGNAATFKLVPQLVPRAVSGAVGWVGGLGAFGGFLIPNGLALFLG
                     TGGRGDPGYARGFWILVLLGLASLGLVRTLAPGAARERS"
     gene            14849..16936
                     /locus_tag="P5313_06950"
     CDS             14849..16936
                     /locus_tag="P5313_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HRY80135.1"
                     /translation="MPKVVRTTCSYCSVGCNFDAVYDDEGKLVSFLPAKDYPVNRGSS
                     CPKGFHLLKALEVPERGATPLLRNLRGDLEPVDWDRAISVFVERFKAVRDRHGPESAA
                     FLSTGQIPMEEMALLGALFKFGMGFVHADGNTRQCMASAAVAYKQSFGFDAPPFCYED
                     LELTDLPIFVGANPVIAHPVLWARLKGRAEKPPVVVIDPRLTKTAREADLHMAVRPKG
                     DLTLLYGLARILVEEDWIDRPFLEAHTEGFEEFSEFVRRFDDARVEAESGIPPARVRE
                     LAERIRSARAVSFWWTMGVNQSHQAVRTAQAIINLCLMTGNIGRPGTGPNSITGQSDA
                     MGSRMFSLTTSLPAGRDFTKEADRIQTAAILGIDPGRIPSVNSLPYHKILEAVDEGRI
                     KALWFVATNPAHSWIDKHTLLHRLAKLEFLVVQDLYPGTETAARAHLYLPAAGSAEKD
                     GTMINSERRLGAVRKVRDAPGQALPDFEIFLRIARAWGCGDLFREWTDPEAAFRILQR
                     LSAGRPCDFSGIRGYEEIRERGGIQWPFPASGADPAPQRRLFADGRFYTDSGRAKFLF
                     SEPQPMPEVPDAEYPFQLITGRGSLVQWHTQTRTGKVAMLRSLAPAEAYADIHARDAR
                     RLGIAEGAAVRVRSRRGEVRVKARISDEVSPGQVFMPMHYPESNFLTFPAFDPYSAEP
                     AYKMGAVALEAEP"
     gene            16933..17538
                     /locus_tag="P5313_06955"
     CDS             16933..17538
                     /locus_tag="P5313_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRY80136.1"
                     /translation="MSAPLFGLVLAGGRSVRMGRPKAGLRYRDEDQAHYLTGLLGSVC
                     EGAWLSVGADPASGAEFGDLPILRDRDPGLGPAAGLLAAWDRLPEAAWLVAAVDLPFL
                     DRPAVERLAGARDPAKAATAALNPDSLLPEPLFAVYEPAFRAHLEAAAAAGRPGLAGV
                     LARLRNRGEGPGVALVRFKDARVLRDVDTREEYRLLSGGVA"
     gene            17535..18182
                     /locus_tag="P5313_06960"
     CDS             17535..18182
                     /locus_tag="P5313_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80137.1"
                     /translation="MRFDTNGTPGPAAAALVWGGLWGAAEATLGFALHLAGRLVPVPG
                     LAGFLMFPLGLGFLLEARRASGSDAAVPLAALTAASVKAASALLPGVGWLFVGNPALA
                     ILAQGLAVYLGGSLLEGRRGAAAFGWGAALALSWRLAFLVLVFLLPMQKGILRKGWPA
                     LAWFLAADSLANGAVIALAAYGPGLSDRARRVLYRPALAPLAVVLGLAAQAAAAL"
     gene            complement(18203..19135)
                     /locus_tag="P5313_06965"
     CDS             complement(18203..19135)
                     /locus_tag="P5313_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRY80138.1"
                     /translation="MRDSDESRLSAGALGAALVVVTVWGFNFTVVKLGLADVPPLFLL
                     TLRFAFCAFPAVFFVKKPAASWGSLAAYGLLLGVGQFGLLFTALALGAPAGLSSVLLQ
                     SQAFFTALLAAALLKERIGMHSVAGMALAAAGLTLVALSGRGGSLSGMTVPLALMILG
                     AAFFWGAANVVVRTMPGADALGLVVWSSVVSPLPLLGLSLWLEGPGPVAASLARPTPA
                     WVGALAYLVIAASLVGYGLWNRLILRHGAGRIAPFSLLVPIVGISSAALVLGERFGPA
                     DAAATALVLAGLLVHVFGGSLAGRLSARFRRPRP"
BASE COUNT         2936 a         6511 c         6886 g         2963 t
ORIGIN      
        1 gagatgctgt gcacctgggc gcgcgatcac ggcgtcgtcg gcaccggcgt cgacctctgc
       61 cgctcgttca gcgagcaggc gagatcgcgg gcgggggaac tcggcgtggc gggccgggtc
      121 cggttcatcc acggcgacgc cgccggttac gtctccgacg agaaggtcga cgtggcagcc
      181 tgtgtcggcg ccacctggat cggcggggga gtcgccggga ccgtcgagct tctgtcgcgg
      241 agcctgcgcc ccggagggat cctcctcatc ggcgagcctt actggcggaa cctgccgccg
      301 acggaggaag tcgccaaggg gtgcctcgcc ggctcgatct ccgacttcct cccgcttccg
      361 gagctcctcg cgtccttcgg ccgcctcggc tgtgacgtcg tcgagatggt cctggccgac
      421 caggacggct gggacaggta cgaggcggcc aagtggttca ccatgcgtag atggctggaa
      481 gccaatcccg gcgacgagct ggcggaggaa gtccgggccc ggctaagctc ggaaccggag
      541 cgccacgccc ggtatacgcg ggtatacctg ggctggggcg tgttcgcgct gatgccgcgg
      601 tgatgcgcga gggtttgacc gtccgccgat taaagattat gataatagta atatatgatt
      661 cttgggccgc atgccgaaag cccggtcttc gggggaggga cctgcgatga agggaatacc
      721 gtacgtcaat ttcaacggga actgcgagga ggccgtacgc ttctaccact ccatcctggg
      781 aggggagctg gagcttctgc gattcaagga cataccggag ggcggcggaa tgcgtccggg
      841 cgggacatgg aacgacaaga tcatgcacgg gtccctgaaa ttcaagggcg gtaactacct
      901 gtacttcagc gattcctggg aggagaatcc ggtcaccgtc ggatcgaacg tgaccgtcca
      961 cctggaggtg gattccgaga aggacgtgta caccttcgtg gaaaaactgg gcgccggagg
     1021 tactatccag atgcccgcgg caaaggtttt ctggggctcc gtatacggaa gcgtcgtgga
     1081 caggtacggg gtctgttggg gaatcgaata ccaactctga tttccgaggt acgttcgttc
     1141 ctaattcgcg gaacgaagga gcgtgtatgg ccggcaaacc ggttccctgt ctctggttca
     1201 acaataccgc caaggaagcc gtggagtact acctctccat cttcaaggga gggcggatac
     1261 tctcgatcga tcattacacg gaggtgggag aagagatcac gggccacaag aagggcgata
     1321 tcgtgacgat cgagttcgag atactggaaa cgaggttcat cgccctcaac gcgggccccg
     1381 agttctcgtt caatccgtcc gtatctttca cgatagaatg ctccgatcaa gaggagatcg
     1441 attactattg ggaacggctg tcgagcaaac ccgaggcgga gcagtgcgga tggctccagg
     1501 atagatacgg cgtatcgtgg cagatcgtcc ccaggatgct ttcggaggcg ttgctgaacg
     1561 gaaccccgga acaacggcgg aggctgacgg aagtctacat ggatatgaaa aaattcgacc
     1621 tcgaaaaact ggaggccgcc ttgaggacgg aaccatgagc ggaaccccca agggcgggac
     1681 gcaggccgaa aaattccggc gcatggtgtt cgcgaaggtc tacccgctgt acctcgagaa
     1741 ggcggcgaaa aagggacgct cgaaggaaga agtggacatg gtgatcagct ggttgacggg
     1801 atacgacgag caagggctgc ggaagcggat cgaggagggg tgcgatttcg agaccttctt
     1861 cgacggggcc ccccggttca atcccgacgc cgtcaagatc acggggctga tctgcggctg
     1921 gcgggtcgag gagatcgagg atcccctggt ccgcaaggtc cggtacctgg acaagctcgt
     1981 cgacgaactg gcccggggca aggcgatgga caagatcctg aggaagtagc tgaccgggcc
     2041 cggggtgtcg tagaataggg atgttcccag accctcgagc aggaggcaat ccatgtccgc
     2101 gtcccttccg atctgccgtg ttcccgaatc gaagaccgcc gtgtccctcg acctctccat
     2161 ggcggcccgg cacggcctgg tgaccggggc cacgggcacg ggcaagaccg tcaccctcca
     2221 ggccctggcc cagggctttt cggaagcggg ggtctcggtc ttctgctgcg atgtgaaggg
     2281 ggatttgtcg ggcatcgccg cgccgggggg gaacaatgcc aagatcgggg cagccttcgg
     2341 caagctcggg cttccggagc cggcctggaa gggctgcccg gcggcgttct gggacgtctt
     2401 cggaaagaag gggcatcccc tccgggccac ggtctccgat ctgggccctc tgctcctggg
     2461 gcgcctcatg gggctgtccg acgtgcaggc ggattccctg cgcctgctgt tccgtatcgc
     2521 cgacgaaaag ggactgcccg tcctggacct ggcggatctc cgcgccctga tcggctgggc
     2581 aacggacaac gccgccgccc tcaaggccga atacggcctt ttgtcctccg ccagcctggg
     2641 cgccctccag cggtccgttc tggtgctgga ggattccggg gccggcaggt tcttcgggga
     2701 acccgccctc cagatcgggg acctgatccg gacggccccg gacgggcgcg gcatggtaca
     2761 cgtcctggcc tccgaggatc tcatggccga gccgactctg tacgccacgg tcctcctcca
     2821 tatcctcggg gagcttttcg aggagcttcc cgaggcgggg gacctctcca agccccggct
     2881 catcctcctg ctggacgagg cccacctcct cttcgacgac gcccccaagg ccctggtgga
     2941 cgcggtggag cggaccgccc ggctggtgcg gtccaagggt gtgggcctgt ggctggtcac
     3001 ccagagcccc ctggacgtgc ccgaatcggt gctgggccag ctgggaaacc gggtgcacca
     3061 cgccctccgg gcctacaccc ccaaggaccg caaggccgtc gactcggtgg ccaacagcct
     3121 tcgtcccaat ccccgggtgg acgccgcggc ggccatcacg gagttggggg tgggggaggc
     3181 cctggtctcc ttcctggacg ccgacggccg tccgaccccg gtggagcggg cccgggtggt
     3241 tccccccgcc tcccgcatcg gccccctctc gcccgaggag cgttccgccc tggtcgcggc
     3301 ctctcccctg ggtacccggt acgacaagcc cgtggaccgc gaatccgcct acgagatgct
     3361 ccggggccgg atggcgtcga ccgggtccgg aacctcaggc ggacaggggg cggggactcg
     3421 ttccccgggg aaggccggcc ccggggcggg ttcccgggaa cccaccatcg aggagatcga
     3481 ggcggagctc cggggctggc cgatgccgtc ccggaccgag ccggagtacc ccgactacgc
     3541 cgaggagcct cccgcccgcc gggcgccggt ctcccggtcc tcggggggga gccggcccgc
     3601 ggcgcgatcc ggttctcggt ccgtccgatc cgggggctac gcggcttcgg ggggctcctt
     3661 cgcggaggac ctggcacgtc tggccctctc cgccgcgggc cgggagttgg ggcggcaggt
     3721 cgtgcggggc ctcctgggca cgccgagccg acgacgccgc tagcacgggg agtcgaaagc
     3781 ccgtgaccgt gctccgggac ggaacgggaa cgcccgccgc ggagcatccg ggaactcgag
     3841 cgggcggtga tcaagagcgt cggagaaaac gagctcatcg gggagaacca gggatgaacg
     3901 tcacggtctg gggttgccgg ggctccatca catcgcccgg cagggatacg ctccggtacg
     3961 ggggagagac gacctgcctt gagatcgtca cggacggcgg cgaccgaatc gtcatcgacg
     4021 cgggatccgg tctccggagg ctcgggcagc atattttgaa ggatcccgac gtcgcggaca
     4081 tcaccctcct tctgacccac gcccactggg accacctggt gggcttcccc tttttccggc
     4141 ccgcgttttt ctccaagtac acgatcactc tctgcggagg gtccgatccc cggatctcgg
     4201 tgctcaaatc cctgcggcac cagatggagc cgccgtattt ccccgtcgac atgagcgtaa
     4261 tgaaggcgaa gttcgtcacc ggctgcacct gcggctgctc ggaatgcgga aacgagctcg
     4321 ccgtagggag cggatcccat cgctgcgaat ccattcccct gagccatccc aacggcggat
     4381 acgggttcaa gcttacggac aaccgggggc gggccttcgt cctgctcacg gacaacgagc
     4441 tgcggtatcg gcacgagacg ggactggacc gcgaagggta cgtggagttc tgcagggatg
     4501 cccgcctcct cttccacgac gcctgctaca ccgaggagaa gtacgagagg acgaggacct
     4561 gggggcattc gacctacaag gacgcgatcg acctcgcgat cgacgccggc gtggagcgcc
     4621 tcgggctgtt ccaccacaat cccgatcgag gcgacgacga gatcgacggg atcctcgggt
     4681 ggtgccgttc gtacatctcg ggacggggga gcggacttga ctgtttcgcc tgcgcggagg
     4741 gaatgaccct ggccgtatga cgaccgctgg ccggttccgg cgaccgggga agccgccgcg
     4801 gaggcttgcg gcgccgcctt cgccgcgcca caatgatagc ggctgcgata ttaccgggag
     4861 gttcccatga tcttcattcc cgccggattg gccgccctgg ccgtcctctt cttcttcctg
     4921 ggccgggggc agggccggaa ggcctacgac atgcagacca ccgagacgag ctcggcggcg
     4981 gaccttgccc ggctggcctc ggacatcgcc caggagatcg gggccggttc cctggcccag
     5041 accgcggaga tcaagggcac gatcgagtgc gacgcgcccc tgtccgcgga gatgtccggg
     5101 accgggtgcg tctggtaccg gtccgtggtg acccgggagt acgaggagcc ctacaccgag
     5161 cgggactcgg acggcaagac tcggtccgga acccgcaggg gctcggagac ggtctcgtcc
     5221 aacgagcgtc gcgtcccctt ccgggtccgg gacgcctcgg gctcctgcga agtggacccc
     5281 gagggcgcgg ccatggacgg ggagagggtc ctgtcccggt tcgagcaagg cgatcaaggc
     5341 ccgagcctca gcttcggggg tctgaccgtc cggttgggcg ggatcggcgc ggggcgcagg
     5401 acgatcggct accggctgga ggagtgggcg gtgccgacgg gccgagcggt ctacgtgctg
     5461 ggtgaggccc gggacgacgg aggccgcctc cgggtgggca agccctccgc caagggcggc
     5521 cgcttcctgg tctccctcaa gaccgaggag gagctggttc gcgccgcgaa gtccggctcc
     5581 acgatcctga cggtgctggc gggactgttc acggccgcgg ccgtcgtagt cctggtcctg
     5641 gtgctgaccg gcgtgatctg aagcccttcc cgaaaagacg ggcgccccga aggatcgggg
     5701 cgcctcttgt ttgactgtac ggtccggtcc gcagaaacct cggcggccgg cctctatttt
     5761 agaccttcgt tctcagaagg ccaggacgat caccgccgcg gccacggcgg caaggctggc
     5821 cgcagcgtag gcgatcaggg tcagggtgct cttcctcata tcgtcctctc ctcggcggcc
     5881 ttcgagccgc ccgtcccggc ggtccgggac cgccaggctt cctgtatggt ttccatgcct
     5941 atgcccagtt cctccatccg gacgaagagc ttcgggagct cctcggagaa gaaccggtcc
     6001 ctgcgctccc gggccgccac ggcgggcccg tcctcggcca ggaagtaccc ggtgccccgc
     6061 tcgcaacggg ccaccccccg gtccgccagg gccgccaggg cccgggcggc ggtgttgggg
     6121 ttcacctgga gggagaccgc caggtcccgg gcggagggca gacgctcccc ggcggtcagg
     6181 gcgccggaca cagcctgggc cgcaaggtcg tcggcgatct gctcgtagat gggggacgaa
     6241 tcattgaacc tcatcgaccg cttccttctc cgccacgcgg aagtacccgt agaccgcagc
     6301 ggcggcccag gagaaccagc cccaggcccg gaagacccag atcaggacgg gctcggaggc
     6361 cgggtcgaaa tcgggccgga accggtagga gaagtgccgg ttccccaggg cttcccggcc
     6421 ctccggggtg gaaaggagca tcccgaggac gaagaggaag cccaggaacg cggcccaggc
     6481 cgagaacagg gcccccgcct tgaccaggga gagcttgccg aaccgggccg aggcggccag
     6541 ggaagcggtc acgaagaaga ggtagccgaa gacgctcccc tcgtccagac cggccagggg
     6601 gttgaagacc acccccgggg aggccttgag cagggccgcc aggccctcca ggatcgcgga
     6661 cagtcccgac gccgccaggg ccgcggccag ggggtacacg accacatacg agacgaaggc
     6721 cgccgcgtac ttttcgaagg acgtggccgg caggaggatc cagtcggacc cgccccggcc
     6781 gttgtgcatc tgggagaagg cccggcccgc caggaggacg ccgcccagcc agatcaggaa
     6841 ggcccagccg ctttcgccgg agtggcggga ggggtggttg aggaacgcgg caccgcccag
     6901 gaggatggaa aggacgttca gacccgccag ggcccccagg gcgatgagga agggggaaag
     6961 gtcctcgagc aggcggttgc gggcgaggag cgcgacgcgc tccggggaaa acggcgtacg
     7021 tacggttgta tcgttcattt gaggtctcct gcgtcggggt tgtaggggcc gggattccgc
     7081 ccttccaggg cggcccggaa ggcctcgggg cgggacacgg cggcggtgaa ggccagttcc
     7141 aggtccgcgc agctgccggg cttggccagg agggccgtcc agcccagggc gtcgcgctcg
     7201 gcgtagatca ccggccggcc ttcaaggccg ggaagccgtt ccacggacag ccggtcggac
     7261 agggccgagg cggggaagga gcagaggatc ttcccgccgt gcaggatgac gacgggatcc
     7321 agtaggttct ccaggtcccg ggcctggtgg gtggagacca cgatcacccg gtccggcgtg
     7381 gacgccgcgg ccagggcccg gcggaactgg gccttggagg ggatgtccag gccgttcgtg
     7441 ggctcgtcca ggaagaccgc ccgggacccg ccggccaggg cggcggccac cgcgaacttc
     7501 ttacgctggc cgtaggacat cttggccacg ctcttccccg ggtccaccgc gaactcctcg
     7561 aggagggacc ggaagagccc ggcgtccatg gccggacgga aggccccgta gcgttccagc
     7621 caggtctccg gggagagggc ggggccccag gggtcctcgg ggacgaagca gacgtccgcc
     7681 aggaccgcgg cggacctcga ccccgggcgt ttcccgaaga cttccacaag cccggaggcg
     7741 gggaacaagg cccccgcccc cagcttgagg agactcgtct tgccggcccc gttcaggccg
     7801 aggaggccga ccgtggatcc ggcctccatc gcaagttcca ggccctcgaa gagcggccgt
     7861 ttgccgtacg cgaacgtcac ggcgtcgaat cgaaccatga ttacctccat ggtgtattag
     7921 tgtagtaata cactagcgtg atcttgaaaa gaagtcaagg ggattcggat gaattttccg
     7981 cggcatccgt ccgcgggaac cggaactcgg cttcgaggag cggtttccgt cccgacgtct
     8041 cgtcttgcgt cccctcgggg cgcccgccta tactttcgcc atgttcgatc cgccgattcc
     8101 cggccggcac gatgtccgga tctgcgtcgt ggggggaggg ggctccgggg ccgccctggc
     8161 ctacgacctg gccctgcggg gcttctccgt ccttctcctg gaaaagggcg agttcacctc
     8221 gggcaccacg ggccgccacc acggtcagct ccactcgggg gcgcggtacg ccgtcgggga
     8281 ccgggccatc gcccgggagt gcatggagga aaccctggtc ctccgccgca tcgtgccgga
     8341 ggcgatcgag tacaacggag gcatcttcgc cgccatccgg gacgacgagg cggactacgc
     8401 ccccgccttc gtgtccgcct gcctcgagtc gggcatcccc gcccgggaga tcccggtcac
     8461 cgaggcccgg gccctggagc cggtcctgaa tcccgcgctc aagcgggcgg tctgggttcc
     8521 cgacggcacc ctcgacgcct accgcctggc tgcctccttc ctggccgcgg cggccgccct
     8581 gggcgcggac ctccgggcct tcacggagct ggtcgggatc gaggtctcgg acggccggat
     8641 ccggggcggg acggtccggg ggcccgacgc ccgggaggag cgggtttcct tcgacgtcct
     8701 ggtcagcgcg accggggcct gggcggggag ggtgggcagc ttggcgggcc tggacatccc
     8761 cgtgaccccc gccccgggaa ccatggtggc ggtgcgggga cggctctcgg acctggtcct
     8821 ctcccgcctc gcgccgccgg gggacgggga tatcctggtg ccccagcggg ggctctccat
     8881 cataggcagc acccagagcc gggccgactc ccccgagggg atcctcccga ccagggcgga
     8941 aatcgagttc ctccgggtcc gggcggacga gctggccccg ggcttttccc gggccccctt
     9001 ccacgcggcc tgggccgcgg ctcggcccct ggcgggacgg gcggcctcgg acgcggaggg
     9061 ccgtgggatc agccgggact tcgccgtcct ggaccacggg gccgaggggg tgtccgggat
     9121 gttcaccctg atcgggggaa aggccacggt cctcagggcc atggcccgga aggccgcgga
     9181 cgcggtctgc ggcttcctgg acgtcgagga accctgccgg accgcggact accccctgcc
     9241 gtcctggagg gccctatgat cgaagtcaac ttcaggatcc gggacgggaa agacttcgtg
     9301 gagctgtccg gaggcttccc ggaggatgcg acggttctgg acgccctgga aaccctccgg
     9361 gcccgggccg agggagctgc cccggcggcc cgggtgcccc actaccggca ctcctgccat
     9421 cacgggtcct gcggaacctg cggggccgta gtgaacgggc ttccgtccct gatgtgcctg
     9481 tcccggcttt cggacctggc cgccccgcgg ccccgggagc ccgggggtcc cgccgtggaa
     9541 ccctcgcacg gaccttccgg agagctcgtc gtgacccttg agcccctgac gcggggaagc
     9601 ctggtcgcgg ggatcgccgc ccggccgacg tcagccctgg agggcatccc ggaggagtac
     9661 tcctacctgc gtccctcgga ggacgcgggc cgggcgtccc ttcccgcgga tccgacccgg
     9721 gatggagaaa agctcccggc cctggacggg gcggttagcc cgcccctttc cggccgggtc
     9781 cgcttcgagg cctgcatcga gtgcggactc tgctccgcgg cctgcccggt gacggccccc
     9841 ttcctggggc ccgcggccct ggcggcggtc aaccgccacc gggagaaggc ccccgaatcc
     9901 gcggaaagca tgctcgccct ggccgccggg gccgacgggg cgcctgcctg tgaaaggcac
     9961 ctgcactgct cccgggtctg tccccaggcg gtgtacccgg gaaagcacat ccagctgctc
    10021 cgaaaggccc tggccgggcg ggctcagtag aacccgattt cccgcacgtg gagccgtgcc
    10081 cggaaggcct tcttgtaggc cgttaccgcc agactcctga gtttcctcac gtccagggga
    10141 aaggccgccc cgaagtcgcc cttcacgaag gcggaccagg ggatgcggat ttcggaccac
    10201 ccttcggtca ccgggagggg ggcactgtag aaggcccagg gaaagaccat ggaggtcgtg
    10261 cgcaggaaga tgtagtagcc gtccccggcg ccccgggcga tcaggcggat gccctggtgc
    10321 ttggacccgt cgtagtggga ggatcttcct tccttcagga gcagacgggc ctggatgaaa
    10381 ccgccccggt tctccagccg cacctccccg gtcaaggcca ggtagggccc ctccgcgtcc
    10441 ccctcgatcc ccaggcggag gtccgagatc cctcccatca cccggtcgga gaagccttcc
    10501 catcgggtcc ccagggagga gatgccggtg cggaagtcgt ccaggagaag cggggtcatg
    10561 ggagctcctt ccgcgggggc ccggggcgcg aacagcgcgg ccagggacag gcacgcgaac
    10621 atggattgga aagacagcgg cttcatatca ggaaaggtat gcatattatg atccgggagc
    10681 aatcctttcc cggtgtcacg aaccttgtga aaaagccgag ggcgccttga ccgtgtttcg
    10741 gaacccctcc tatactccgc ccatgaaatt cgccccggcc ctcctggcgg ccctcgccct
    10801 cgcttcctgc gcggcggccc cggcgacacc gccggccgac tcggcgcccg cgactccggc
    10861 agccccggtg ctctccgccg gagacgaggc ggcgatccgg gcggccgtgg ccctccgcgc
    10921 ccgggccgtc gcggaaggcg acaaggccct gtacctgagc ctcctggccc cgggcgaccc
    10981 ggccttcctc ctggagcagt cccggtggtt cgactaccgg ctttccgcag tactatccga
    11041 gttcatcctg gaggtcgagt ccctggaggc ctcagggccg ggggaatgcg cggtcgccct
    11101 ccgccagaga tacctttatg ggccgaacgg ggaggtccgg gactgccgct tcgtccagct
    11161 ctaccggaag gtcgacgaaa cctggctgga cgcggacatc gccttccgga ccctccaatc
    11221 cgggcacatg gaggtccggt acgacgaggg agtggacggg ctccgggcgg cccgcatcct
    11281 gggggacgcc gagaaggcct ggaccacggt ccgggattcc tacggtgagg cccctgcccg
    11341 caggaccgtg gtgaaactct tctcgaccca ggaactcctg cgccagaaca ccaagatcac
    11401 catcggccgc ctgttctacg gctgggggga gcccggggag tccatcaagc tcttcgtccg
    11461 gcccgacccc tcccgctcct acgtccccgt gctggcccac gaactggtcc acgtcgtcac
    11521 cctggagacc agcaagaacc tctgctcctg gttcgccgag ggcctggcga accgattcgg
    11581 gaacttcgcc gtcctgggcg ggacctacct cgactcgggg taccacaagc cctccgagta
    11641 cgaccgcagc gtccgatggc tcgggtccgt ggactcccag accatcgacg acgaatacga
    11701 gtgggggatc tacgggggga tgtccggggc ggtcgtgggc ttcctggccg agcgctacgg
    11761 cccggacgcg ccccgggccg tggtccgggc cctggccgcg tacccccagg tccaggaagg
    11821 ctatgtgtac gccgagcacg acgcccggtt ccggggctac ctcgacgggg cgatgaagac
    11881 ggccctgggg gtcggcctcg aggaattcga cctggcctgg cgggagtgga tccggaaccg
    11941 ggtgaaatga gccggttctc cggccccccg gacgcgccgg gctcgaaggg gagacgtcca
    12001 tgcccacgat gaacgagatc tacgccgccc acgccgaccg ctacgacgag ctggtatccc
    12061 gggaagaccg ggagggcagc ctgggccggg agctcgggcg cctggtgccg gcgggggccg
    12121 ccgtactcga actgggggcc gggaccggga gggtgacgcg cctctacgcg ggccgcgtcc
    12181 ggagcgccct gctctgcgac cgctcggccc acatgctcgg ccgggcccgc tcgaacctcg
    12241 cggatttccg ggaccgcctg gagttccggg tactggacat ccgggaggcc ggaaccctgg
    12301 agggcggatt cgacgtgatc ctggagggct gggccctggg gcactgcgcc ctggaggaat
    12361 acgaaaggct gtcggatttc ctttcgggaa ccctggcggc cctggacgcc ctgctggcgc
    12421 ccggggggcg gatcgtcctc gtcgagaccc tcggcacgaa tgcggaccgc cccgcccctc
    12481 cgggagaaag gctggctcgt ctctacgatc ttctggagcg ggaccacggg ttccgccgca
    12541 cggtcatccg gacggactac gagtttccca cggtagagga ggcccgccgg gtcctgggct
    12601 tctttttcgg ccccgacagg gaggggcgga tttccgatcg gatcgtgccg gagtggacgg
    12661 ggatctgggt tcgggaaagg ggttgagatg gaagcgatga ggatcgcacg aaaggacgga
    12721 accttcatca ccaccgaatt ccacgggaac ggctcgccgg tcctgggtgt ggtggtgtcc
    12781 ggcatgggct acacctaccg gaatcccctt ctgtactacg ccgtgagcct tctggcggaa
    12841 tccggggcgg actgcctcct ggtggacctg cggtactacg aggacgaagg gttccgggcc
    12901 ctggacgagg cggggaagga cgcccgcttc gaggccgacg ccgcggcggt ggcggacgcg
    12961 gtggagcggg aagcgtcccg cagggagcgg atcgtcctgg ccggcaagtc cctgggcacc
    13021 agcgtgatcc gccgctgcct gcgcaggccg tcggtcgccg agaagtcctc cctgatcctc
    13081 ctgacccccg gctcggagtg gtccgccttc atccccgagc tggtacggtc ggggcggccg
    13141 gccctggtgg tcgggagttt cggggacagg tactacccgg tcccgaacct cgacgagctc
    13201 cgggcggccc cccgggtgcg ccggctggaa ctggaggcgg gggaccattc cctggagacc
    13261 ggggacgcgg tccgggacgt ggaaaccctg gggcgggtgg ttcaggtcat gaaggagttt
    13321 ctgacggaga cggctgggcc ggcccggtaa tctgggccgc ggggggacgc ccgctccctc
    13381 cgccgattca cgtttccatc cgtccattcc ccgggcccgg cgtttgagcc ttaggttgaa
    13441 aaattctcgc gattttcctt gcattctgac tttgccgtat taaacttatt aaagtaccga
    13501 aatacccaaa tatgatcatc ccgtacgagc acggtacgag gaggcggttc atgggtgata
    13561 cgagagtcgc gggaaccccg gcccggggcc tggcgggagc aaccctgggg ttcttcatcg
    13621 gctttgcggc ggtatccttg ttcggaccca cggtccggta cctgcaggag gcggcgggct
    13681 tcggtccggt ccaggcgggc ctcctggttt cgatcccgag cctgacggga tccctcctca
    13741 gaataccttt ttcagctctg gtggacacca ccgggggacg taagcctttc ctggtcctcc
    13801 tgggcctttc ggccgcgggc ctggcgggcc tctggttcct gctctccggg gatcccgcgg
    13861 gccttccggg acgcttcccc ggtctcctgg tcttcggggc cctggggggc tgcggcatag
    13921 ccaccttctc cgtggggatc agccagacct cctactggta tccccaacgg atgcaggggg
    13981 cggccctggg ggtgtacgcc gggctcggca acctggcccc cgggatcttc gccttcctgc
    14041 tctccgccct gtccctgccc ctgctgggcc tgtccggatc ctacctggcc tggctgggga
    14101 tcctggccgc gggcaccgcg ggctacgccc tgctggggcg gaacgccccg tacttccagc
    14161 tgcggtcctc gggcctgggc cccgaggagg cggcccgccg ggcccgggaa gaccacggcc
    14221 aggagctctt cccccggggg agcgtccggg aaagcctggc cgcctccgcc tcggagtgga
    14281 ggacctgggc cctgacgggg atctacttca tcaccttcgg gggcttcctg gccctgaccg
    14341 cctggctgcc caagtactgg ggctcctact tcggcctctc cctgggcgcc gcggggagcc
    14401 tggctgccct cttctcggtc tccgcctccc tgttccgggc ggcaacgggg ggagtctcgg
    14461 accgcctggg aggggcgagg accctcgccc tggccctggg ggtcatcgtc ctgggctccg
    14521 cggggctggc cctgtcgggg tccgcggtcc tctccgccgc ggccagcctg gtcctggccc
    14581 tgggcatggg ggccgggaac gcggcgacct tcaagctggt gccccagctg gtcccccggg
    14641 ccgtgtccgg ggcggtgggc tgggtggggg gcctgggggc cttcgggggc tttttgatcc
    14701 ccaacggcct ggccctcttc ctgggaaccg ggggccgggg ggatcccggg tacgcccggg
    14761 ggttctggat cctggtactt ctgggcctgg cctccctggg tctggtccgg accctcgccc
    14821 caggcgcggc ccgggaaagg agctgaccat gcccaaggtc gtccgtacca cgtgcagtta
    14881 ctgttccgtg ggctgcaatt tcgacgccgt ctacgacgac gaggggaagc tcgtgtcctt
    14941 cctccccgcc aaggactatc ccgtgaaccg gggcagctcc tgccccaagg gcttccacct
    15001 gctcaaggcc ctggaggtcc cggaacgggg ggcgacgcct ctcctgcgga atctccgagg
    15061 ggacctggag cccgtggact gggaccgggc gatatcggtc ttcgtggaaa ggttcaaggc
    15121 cgtccgggac cgccacgggc ccgagtccgc ggccttcctc tccacgggcc agatccccat
    15181 ggaggagatg gccctcctgg gggccttgtt caagttcggc atgggcttcg tgcacgccga
    15241 cgggaacacc cggcagtgca tggccagcgc ggcggtggcc tacaagcagt ccttcggctt
    15301 cgacgccccg cccttctgct acgaggacct ggagctcacg gaccttccga tcttcgtggg
    15361 ggccaatccc gtcatcgccc acccggtgct ctgggcccgc ctgaagggcc gggccgagaa
    15421 gcccccggtg gtggtcatcg atccccggtt gaccaagacc gcccgggagg cggacctcca
    15481 catggccgtg cggcccaagg gggacctcac tctcctctac ggcctggcaa ggatcctcgt
    15541 cgaggaggat tggatcgacc ggcccttcct cgaggcccat acggaggggt tcgaggagtt
    15601 ctcggagttc gtccgccgct tcgacgacgc ccgggtggag gcggagagcg gcatcccccc
    15661 ggcccgggtc cgggaattgg cggagcgcat ccgctccgcc cgggcggtgt ccttctggtg
    15721 gaccatgggt gtgaaccaga gccaccaggc cgtccggacc gcccaggcga tcatcaacct
    15781 ctgtctcatg accgggaaca tcggacgccc cgggacgggg ccgaactcca tcaccggcca
    15841 gtccgacgcc atgggctccc ggatgttcag cctgaccacc tccttgcccg cgggccggga
    15901 cttcacgaag gaggcggacc ggatccagac ggcggcgatc ctgggcatcg accccggccg
    15961 catcccctcg gtgaacagcc tgccctacca caagatcctg gaggccgtgg acgaggggcg
    16021 gatcaaggcc ctttggttcg tggccacgaa tcccgcccat tcctggatcg acaagcacac
    16081 cctcctccat cgcctggcca agctcgaatt cctcgtggtc caggacctct atcccggcac
    16141 ggagaccgcg gcccgggccc acctctacct gcccgcggcg gggagcgcgg agaaggacgg
    16201 gacaatgatc aattcggagc gccgcctcgg ggcggtccgc aaggtccggg acgccccggg
    16261 ccaagccctg ccggacttcg agatcttcct gcgtatcgcc cgggcctggg gctgcgggga
    16321 cctgttccgg gagtggaccg accccgaagc ggccttccgg atcctccagc ggctgtccgc
    16381 gggccggccc tgcgacttct cggggatccg gggctacgag gagatccggg aacggggggg
    16441 gatccagtgg cctttcccgg cctccggggc ggatccggcc ccccagcggc gtctcttcgc
    16501 ggacggacgc ttttacacgg acagcggccg cgccaagttc ctcttttccg aacctcagcc
    16561 catgccggag gtcccggacg cggagtaccc cttccagctc atcacgggcc ggggcagcct
    16621 ggtccagtgg catacccaga cccggacggg caaggtggcc atgctccggt ccctggcccc
    16681 cgcggaagcc tacgcggaca tccacgcccg ggacgcccgg cggctgggca tcgccgaggg
    16741 ggcggccgtc cgggtgcggt cccggcgggg tgaggtgagg gtcaaggccc gaatatccga
    16801 cgaggtctcc cccgggcagg tcttcatgcc catgcactac ccggaatcca atttcctcac
    16861 cttcccggcc ttcgatccct acagcgcgga gccggcctac aagatggggg ccgtggccct
    16921 ggaggcggag ccgtgagtgc gcccctcttc ggcctcgtgc tggccggggg ccggagcgtc
    16981 cgtatgggcc gcccaaaggc cggcctccgg taccgggacg aggaccaggc ccactatctc
    17041 acggggctcc tgggctcggt ctgcgagggc gcctggcttt ccgtcggtgc ggacccggcc
    17101 tcgggggcgg aattcgggga cctgcccatc ctccgggacc gggatccggg cctgggtccc
    17161 gcggcgggcc tcctggccgc ctgggacagg ctgccagagg ccgcatggct ggtcgcggct
    17221 gtggacctgc ccttcctgga ccgcccggcg gtggagcgcc tggcgggggc ccgggatccg
    17281 gccaaggcgg ccacggcagc cctgaacccg gattccctcc ttccggagcc cctcttcgcg
    17341 gtctacgagc cggccttccg ggcccatctc gaggccgcgg cggcggccgg gaggccgggg
    17401 ctggcggggg tcctggcccg tctccggaac cggggggaag gcccgggggt ggccctggtc
    17461 cggttcaagg acgcccgggt cctccgggac gtggatacga gggaagaata ccggcttttg
    17521 tcgggaggtg tcgcatgagg ttcgatacaa atggaacgcc cggccccgcg gccgcggccc
    17581 tggtatgggg aggcctctgg ggagccgccg aggcgacctt gggattcgcc ttgcacttgg
    17641 cgggccggtt ggtcccggtg ccggggctgg cgggattcct gatgttcccc ctgggcctgg
    17701 gtttcctgct ggaagcccgc cgcgcctcgg gttccgacgc ggccgtgccc ctggcggccc
    17761 tgacggccgc ctcggtcaag gcggccagcg ccctccttcc gggggtgggt tggctcttcg
    17821 tggggaatcc ggccctggcc atcctggccc agggactggc cgtgtacctg ggcgggagcc
    17881 tcctggaagg acgccggggc gcggcggcct tcggttgggg cgccgccctg gccctgtcct
    17941 ggcgactggc cttcctggtc ctggtcttcc tcctccccat gcagaagggg atactccgaa
    18001 aggggtggcc ggcccttgcc tggttcctgg ccgcggactc cctggccaac ggggcggtca
    18061 tcgccctggc ggcctacggg ccgggccttt ccgaccgcgc aaggagggtc ctgtaccgcc
    18121 ccgctctggc gcccctggcc gtggtcctgg gactggcggc ccaggcggcc gcggccctgt
    18181 aggatcggtt tcgggaggaa tctcagggtc gcggccggcg gaatcgcgcg ctgagacgcc
    18241 ccgccaggct ccccccgaag acgtggacca acaggcccgc caggaccagg gccgtggccg
    18301 ccgcgtccgc gggaccgaag cgctccccca ggaccagggc cgcggaactg atccccacga
    18361 tcgggaccag gagggagaag ggggcgatcc ttcccgcccc gtgccggagg atcaggcggt
    18421 tccaaaggcc gtagcccacc agggaggccg cgatcaccag gtaggccagg gccccgaccc
    18481 aggccggggt ggggcgggcc agggacgccg cgacggggcc gggcccttcc agccagaggg
    18541 agagccccag gaggggcagg ggggagacga cgctggacca gaccaccagg cccagggcgt
    18601 ccgccccggg catcgtgcgc acgaccacgt tcgcggcacc ccagaagaag gcggcgccca
    18661 ggatcatcag ggccaggggg accgtcattc ccgagaggga gcccccccgg ccggacaggg
    18721 cgaccagggt caagcccgcg gcggccaggg ccataccggc cacgctgtgc atcccgatcc
    18781 gctccttgag aagggccgcg gccaggaggg cggtgaagaa ggcctgggac tgcaggagga
    18841 cggaggaaag tcccgccggc gcacccagcg ccagggccgt gaagaggagg ccgaactgcc
    18901 cgactcccag aaggagtccg taggccgcca gggagcccca ggaggccgcg ggctttttca
    18961 cgaagaagac cgccgggaag gcgcagaagg cgaatcgcag ggtcagcagg aacagggggg
    19021 ggacgtccgc cagccccagc tttaccaccg tgaagttgaa gccccagacc gtcaccacca
    19081 ccagggcggc ccccagggcg ccggcggaca ggcggctttc gtcggaatcg cgcatggacg
    19141 tcagtatagc ggtccgggcg gggacgcgcc atgccggggg cccggaagcg ggcaggcccc
    19201 gcggcacggg cgggccgcgt tgcgctctcc gccgcgggcg cctatactca tccaacatcg
    19261 tgaaatcgga ggcatccgtg aaaaagttgg cctggc
//
LOCUS       DAOTEI010000052        19253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1880_length_19253_cov_4.515887, whole genome shotgun sequence.
ACCESSION   DAOTEI010000052 DAOTEI010000000
VERSION     DAOTEI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 19253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19253
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1880_length_19253_cov_4.515887"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="P5313_06970"
     CDS             <1..1215
                     /locus_tag="P5313_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80139.1"
                     /translation="ATGRRNPAATVRALDLGILDDAQAVTEFFGFPSLSWEDLRDLRG
                     FLRGPEGRAAFASAFSGYSGVLGSDPDGDGYFETRSTVRDGAVESWLLDFDQDGLPEL
                     SLRFLDDLPGSGRMTLTGAETVFEYGEYPYLNRVLYRTSSGVREYLFSPDALAFPAVE
                     LFAGPGRGSGEGFAPRRTDAAFPTEAACAAAAFRVTARPEDRGSPVSVTDLDRGVPVR
                     GRIRTPDGRDGILVYRNGMPQYELADMDGDGLYESRFLYPRDPKRAASGTVSSPYRFE
                     ADFNLDGVFEYAENLTFPFERTWDYDGDGRTDARATALGDGGELREFSRRFDGRLDTA
                     VLVRGGRVQRVLRAGVELPLVPDAGGAVLWVGRKPFDFGTVLPEPGFGRRGAERYRIV
                     RFGDQILAESAE"
     gene            1212..2990
                     /locus_tag="P5313_06975"
     CDS             1212..2990
                     /locus_tag="P5313_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY80140.1"
                     /translation="MRARSLPAAALLAAALSVGCASDPAPAPAPRYESLKLEALERLA
                     TEDPAEGMEAALHLRAGSSILSADQLEDSLRRSAAALVRRYEASLAAGDFSAARADAR
                     SLRFLSEDPRTASALPAGFSLGVSDAELTFRMAERLWGEGRETSGLLVFLEALDAGYR
                     DPASLRTFAERAHALGNRQALKSIETVDPSALTGEARTWSSGTVSMAEMRRGTVTIWV
                     NRGIRIAQGIGTPDRGIGSGFFVDRRGHLLTNYHVISSEVDPEYEGYSRLYIRLPGQT
                     TDRIPAKVIGWDRVLDLALIKVDLEPPYVFSFTGRREFVPGDRIYAIGSPVGLDNTLT
                     AGIVSAVGRRFLPLGEVIQVDAAVNPGNSGGPLLDAEGELAGVVFAGLPRYQGLNFAV
                     PAHWAVRVLPDLYRGGEIRHPWLGIAAYEGREGLAAIYRHPDGSPAAPAAGELVRIAG
                     KACKTLSEAQAILLSYDPGTLVPVDYREGGEVRRSWILLRPRPFSPGETAVRRDLRER
                     LLAPFFGMAVTATGKNSFVVERVLPGTIADEAGLSERDPLVIKDIVVSKEDRALLMSL
                     YVKKRKAGFLESIIQIAATLDLDSFL"
     gene            3047..3370
                     /locus_tag="P5313_06980"
     CDS             3047..3370
                     /locus_tag="P5313_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80141.1"
                     /translation="MAKDPAPQELAPVGKLFIDTAAELRAALTKAFSKKGSVLFRWDG
                     VEDLDVPLLQLLYAARVEASRTGKEFHFTGIVTDRVANRLYSAGFVSVIPLSGEELES
                     NLVGF"
     gene            3380..5518
                     /locus_tag="P5313_06985"
     CDS             3380..5518
                     /locus_tag="P5313_06985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRY80142.1"
                     /translation="MLDKFKSSFREEAEELLGQLESTLLELEDSPSDAELLNAAFRAI
                     HTIKGSAGMFGFDEIARFTHDLENVLDKARAGKIAISREFIDLALRGRDHIRGLLSSG
                     DELTEDLRRTTDALVEECRALAAETEARNEASPGSPGAAAPEQPASGAPGAASEGKRT
                     FYIRFQPGPDIFRNGTRVLSLLADLAELGEATLVPHVHDIPRLSEMDPERCYCYWEGY
                     LTTESDRNAIRDVFIFVESESSLTIEEIEETRINDSRTKRLGELLVQYQHVPPEEISK
                     ALSSQKRLGEILVEKGIISKDALDTTLAGQNHAKKIQDKAAEAGGALASVRVASEKLD
                     QLVDLVGELVTLQARLARTSLDLKEISLAAISEQFERLIAQLRDNTMSIRMLPIGSTF
                     NKFRRVVRDLSTELGKEADLLTEGADTELDKTVIERLNDPLVHIIRNSLDHGIESPQD
                     REAAGKNRKGTILLKAQHSGAHVLISVRDDGAGLNAEAIRAKAVERGIVAPGQELTEA
                     EINQLIFHPGFSTAKSVTKVSGRGVGMDVVKREIDSLGGTVMISTERGRGTEMTLKIP
                     LTLAIIEGLLVAVGGEYYVVPLSSVDGCIEIPKEDLRKVGERRIIPYRGDLLPFVDLR
                     SYFEVPGETPDIAQIVIANAQDSRLGFVVDNVIGDYQTVIKPLGRMYKDVSGISGATI
                     LGDGTVALILDVNRLSQAVQAESQRKVQAG"
     gene            5522..6385
                     /locus_tag="P5313_06990"
     CDS             5522..6385
                     /locus_tag="P5313_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRY80143.1"
                     /translation="MTAESASGASIAHLASLDEREFRRLAAFIERELGIRMPDSKRVM
                     LESRLQKRLRLHGLAGFREYVDYVFSEQGSRTEMINMIDAVTTNKTDFFREADHFDFL
                     RSRILPRMAENGAGRVLNFWSAGCSSGEEPYTLAMVLEDFRSGSPRTDFRILATDIST
                     RVLEAAQRAIYDMEKAEPIPYEYKKRFLFRSKDPDRAEVRIRPELRSKVEFRRVNFMD
                     DTYPVPSGFDTVFCRNVIIYFDRPTQERILGHICAHIRPGGWLFIGHSETLTGMDLPL
                     RSAAPTIYQRE"
     gene            6403..7479
                     /locus_tag="P5313_06995"
     CDS             6403..7479
                     /locus_tag="P5313_06995"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRY80144.1"
                     /translation="MAKSIKVMIVDDSAVVRQTLKEILEMDREISVQAVASDPIFALK
                     KLETEKPDVIISDVEMPRMDGITFLKTVMERYRIPVVICSSKTEEGSDNALKAMEYGA
                     IEIIQKPKLGTKQFLEDSRVEICDVVRAANMSRPQERRAPTRVPEVSQKLTADVMLPK
                     PGPERGALETTERVVVVGASTGGTEALKEFLEALPEDSPGIVIVQHMPEHFTAAFARR
                     LDGLCRLTVKEAEDNDTVIRGRALIAPGNRHTLLKRSGARYYVEVKDGPLVCRHRPSV
                     DVLFRSAARYAQKNAVGVIMTGMGDDGARGMKELHESGAYTIAQDEATSVVFGMPNEA
                     IKMGGVDLVLPLSKIFEEVLRRTR"
     gene            7504..7782
                     /locus_tag="P5313_07000"
                     /pseudo
     CDS             7504..7782
                     /locus_tag="P5313_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968266.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     assembly_gap    7783..7792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7793..7880
                     /locus_tag="P5313_07005"
     CDS             <7793..7880
                     /locus_tag="P5313_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY80145.1"
                     /translation="GKEAGATGWIVKPFSADKLLGVVKKVID"
     gene            7890..8378
                     /locus_tag="P5313_07010"
     CDS             7890..8378
                     /locus_tag="P5313_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY80146.1"
                     /translation="MEETSGRTGEYLCFFLGQEQYAVNVSRVEVVLEMQNVTRVPKAP
                     PHMRGVINHRGSVVPVVDLRMRFGMGPSKLAEGTSIIILQIEYGGDLLTVGVLADGVR
                     EVVNLDPADTEKPPRLGSRLDEAVIEGIAKRGDEFVILLNIDEAFREGVPGTAVPAGT
                     DR"
     gene            8388..9932
                     /locus_tag="P5313_07015"
     CDS             8388..9932
                     /locus_tag="P5313_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY80147.1"
                     /translation="MADGAAIVIVEDEFIVALDIQRYLERNGYSVKGILPSGEEFLDR
                     VEDLKPDLVLMDIKLQGKLDGVETAALLADRWAVPVILLTAYADEPTLARAKITQPFG
                     YLLKPFNEQELRTSIEIALYRSMMEKRLRESERLLRQAQKMEAVGRLAGGIAHDFNNI
                     LTAILGYANLLGEEVHGNPKLQDDVEGVKKAVKRAEALTRQLLAFSRRQPMEPKVIQP
                     REALDEMHRMLERLLPANVHLILRNGERLPCIRIDPIHLEQIILNLIVNARDAMPEGG
                     QIEVRLTPCGLDSSRAGSLERIPEGSYVVLTVQDTGTGIPPSDYERIFEPFFTTKAKE
                     GGTGLGLATVYGIVKQSNGYIDLESRVGEGTSFSLYFPGVQYEAVRESSREREARVDQ
                     GTESILVVEDDDEIRSLMERALARRGYSVLTAAHPGEAILVSERHAGRIDLIVADIGL
                     PIMSGSDLVKRMRTVLPGVKALFVSGYPENAPDLPDSAFLPKPFTEAVLAAAVRRLLD
                     SPAAKP"
     gene            complement(10115..10825)
                     /locus_tag="P5313_07020"
     CDS             complement(10115..10825)
                     /locus_tag="P5313_07020"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80148.1"
                     /translation="MPYSLFELRKAFGDLPVLDGFSLDLPERKVTAILGPSGCGKTTL
                     LHILSGLIPPDSGERRGLGEARFSYVFQEPRLVPSLTALENVELVLRSSFGVPERRER
                     ALRFLEGVGLSEARDQRPRQLSGGMRQRVSLARAFAYPADILLLDEPFQSVDLRTRTG
                     LMDAFLELQTADPRTVVFVTHEVKEALYLGDIVTVLSDKPARILDRKELLLPRERRRY
                     GSADLSDVEAALYRLILG"
     gene            complement(10806..11588)
                     /locus_tag="P5313_07025"
     CDS             complement(10806..11588)
                     /locus_tag="P5313_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY80149.1"
                     /translation="MRKSRGAEAARGIPGSAAAAAGALGFLLLWELAARTIGSEIVLP
                     SPLRVARAAADLFPTARFLAALEGTFLRGLGAFGITMAAGVAVGLAVGLSPVLASALS
                     PGMTVIRATPVLAVILLALIWFPAGAVPVFAAVLMAFPVVVADVRQGVGAADPGLLEM
                     ARLFRLRKRDRIRTIYVPALAPHLASASHGALGLCWKVVVAGEVLSQPARALGTGMQG
                     ARIQLETAEVFAWTLAGILLCALTDAAFKLLRRKAARALLPV"
     gene            complement(11566..12561)
                     /locus_tag="P5313_07030"
     CDS             complement(11566..12561)
                     /locus_tag="P5313_07030"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY80150.1"
                     /translation="MNLRFSRAMSSFLLAALGVLPSLALGGREAPAGTVLRLGALKGP
                     TGIGMVRVFEAPPKLAAGDSLELQTVPSADAMAAKLVAGELDAAVLPVNLAAKLYASG
                     LPYRLAAVVGMGMVSVVTTDPEVRSFEDLRGREIHVAGQGATPEYLIRTIAEARGLDP
                     DSDLALAFRLPYPEIAASLVAGRISLAVLPEPFATQALAGNPRARIPFSLDQLWREAT
                     GRPGYPMSVLVIRDSVIRDRPATVRALLGAYADSIAWVKANPAEAGALVEKHDLGLKA
                     AVAAAAIPRSAYLFLPAPEARADVEALLSVFLNIEPKSIGGRLPDSGFYAEIPRR"
     gene            12664..13239
                     /locus_tag="P5313_07035"
     CDS             12664..13239
                     /locus_tag="P5313_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRY80151.1"
                     /translation="MICDRCGKREASVFIRREGSGEFSLCAECAREQGISAEDGRLRI
                     SLEDLFSPPPESPERPGRRTVCATCGIRLEEVRKSGRIGCPACYDSFREELTAFLQRR
                     GRKGPYRGAVPRRFAAFGKPSAGLSPADASAVPVGTAGPETRHNPAAVRDPGPAREDL
                     SGRLRDALDAEDYELAARLRDLLRSLGEEAS"
     gene            13236..14306
                     /locus_tag="P5313_07040"
     CDS             13236..14306
                     /locus_tag="P5313_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80152.1"
                     /translation="MSGPLDDFLSRLPAPAGDGAAINCRVLLRRGLAEHLFPGRADEE
                     QATRTSEVLESALAGTGRFDMLKLSRADSLDRRAAVERDLVPQSYAVRGGGLLAFDRE
                     GSAWCTVNETDHLRLRAEASGADLAGAYRRAAELDALLAESLDWAFSPDAGYLCAEIA
                     DWGTGLSASVTLHLPALAMAGLVDRALRTALDSGLSVHGFWSDESASSGALYELETPR
                     GAWTDEESILVRLGDAVERVGAYEKGAREELLAHDPETLRDAVFRAYGTARYARRIGY
                     DEGAETLSRLRLGAWAGLIAGLEGPKASRGFYGLRNAHLLKYLAKGDAPVGDGPDTVR
                     IEEVRARVLRTLVEDATLVEAP"
     gene            14308..16815
                     /locus_tag="P5313_07045"
     CDS             14308..16815
                     /locus_tag="P5313_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRY80153.1"
                     /translation="MFKGLTQRAQKILSVLAQEEAKRFHADQLVPEHVLLAVLREGEG
                     IGYRALRSLKIDTTEIRIELEKTASGRKGGFMLGDVPLSRRLKSLLETAAEEAHLAGS
                     EYIGTEHLVIASAREGGSVFESLLGRYGVYYEQLRGAVKYLTASREESAAREERTAAP
                     KPAARTPPVAGTVRTPLLDEFARDLTELARADRIDPVVGREREIRRVVRILARRTKNN
                     PVLIGEPGVGKTAIVEGLALRIVAGQIPDALAEKRLLTLDMASVVAGTKYRGQFEERL
                     KRIMREIAQAGNVILFIDELHTVIGAGSAEGTIDASNMLKPALSRGEIQCIGATTLDE
                     YRKYFEKDAALERRFQSVLVEEPTVAETVEILAGIQERYERFHGVTYSRDAVEAAAVL
                     AHRYLTERFLPDKAIDLLDEAGSKRKIENPSRPPELPRVEEEIQRLTEEKLALVSTQN
                     YERAAEIRDSIRKLKARLEEIRNSWGADPGRDRALVTERDLREVISEITGIPLDRLEE
                     SEAGRLLGLEEALHARVVGQEDAIRAVSTAVRRSRAGIADHRRPTGSFIFLGPTGVGK
                     TLVAKTLAERLFGTEEALIRVDMSDFMEKHNASRLVGAPPGYVGYDQGGMLTEKVRRR
                     PYSVILFDEIEKAHPDVFNLLLQLLEEGELRDNLGHTVSFRNCVVIMTSNAGTRDIAR
                     GKRLGFHAEDPRFDLESVKEAAMSELKRQFNPEFLNRVDEVVVFHPLSKEQAGAVLDL
                     MIADLKKRLAEKGLDLEIRKPAHDWLVEKGYDATYGARPMRRLITRALEDPIAADMIA
                     GKYSPGWTVLAELKGEELVVRPRKSPRPRNEPVTARR"
     gene            complement(16923..18362)
                     /locus_tag="P5313_07050"
     CDS             complement(16923..18362)
                     /locus_tag="P5313_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HRY80154.1"
                     /translation="MSLNHKEIDLVLSELDLVGAQIQRVVQPAYDTLVLSLYKPGCPT
                     DLLVCLAHGACRIHSTSIPAPKSEKPLRFAELLRSRIRNGRIEAVRQLGAERIVRLDI
                     RGPADPEAPDPVRYRLYARLWSGAANLILTDDAGSIVDALARKPKRGEIAGGVYRPEE
                     APAGASPEKPFEVRDIPGPGTFNERVDRLYAQGRGELSRDQLLAKVREWFARRRASLE
                     ARIEELRRTETEYADAERLRELGDILMGSVGTGSTEGGYLEAPDFYGGGTIRIRIDPK
                     KSLVENAQAYYERYRKAGSGLADVRAELAETLRRLEVLEAEAVRLETEESPYALRAFL
                     ARRRAAAAARRPYPGIALERDGWTLLVGRSAKENDELLRRHVRGNDLWLHARDWPGSY
                     VFVKNRPGKSVPLEILLDAGTLALYYSKGRSSGQGDLYYTRVKYLRRAKDGPKGLVIP
                     TQEKNLHVTLREDRLRVLRSLIGKEPDEG"
     gene            complement(18549..>19253)
                     /locus_tag="P5313_07055"
     CDS             complement(18549..>19253)
                     /locus_tag="P5313_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRY80155.1"
                     /translation="AQDIADELLAAYGDSRRADDGKAWTELQTGLRESFNYQLPSPPP
                     SREPEKLRDLILEALRSDFEAKRAYAGEANLNHFIRFQYLQAIDQKWLDHLENMEALR
                     EAVYLRHYAQKNPLLEYKIEGFEIFEKMVDDIRRSIATRFFLVRIKTETDRRYVPAAR
                     PGTAVHDEMGQFHGGGDMQDGSARSAAPRSAAPSGTLAAAAGQVVTVKRTVPKVGRND
                     PCPCGSGKKYKHCHGA"
BASE COUNT         3213 a         6366 c         6686 g         2978 t
ORIGIN      
        1 gccacggggc ggcggaaccc cgcggcgacg gtccgggcac tggatctggg gatcctggac
       61 gacgcgcagg cggtcacgga attcttcgga ttcccttccc tgtcctggga ggaccttcgg
      121 gacctccggg gcttcctgcg cggcccggaa ggccgggccg ccttcgcttc cgcgttttcc
      181 ggctattccg gggtcctggg aagcgacccg gacggcgacg gatacttcga gacccgttcg
      241 accgtccggg acggcgccgt ggaatcctgg ctcctggact tcgaccagga cgggctgccg
      301 gaactctccc tcaggttcct ggacgacctt cccgggtccg gcaggatgac cctgacgggc
      361 gcggagacgg tgttcgagta cggggagtat ccctacctga accgtgtgct gtaccggacc
      421 tcctcggggg tgcgggaata cctgttctcc ccggacgccc tggcctttcc ggcggtggag
      481 ctcttcgcgg gacccggacg gggttcgggg gagggattcg cgccccggcg aacggatgcg
      541 gcgtttccga ccgaggcggc ctgcgccgcc gctgccttcc gggtgacggc ccgccccgaa
      601 gatcgcgggt cgcccgtgag cgtgacggac ctggatcggg gcgttcccgt acgcggccgg
      661 atccgcaccc cggacggacg ggacgggatc ctggtctacc ggaacggaat gccccagtac
      721 gaactggcgg acatggacgg ggacggcctg tacgaatccc gcttcctgta cccccgggac
      781 ccgaagcggg cggcctccgg gaccgtgagc tcaccctatc ggttcgaggc ggatttcaac
      841 ctggacgggg tcttcgagta cgccgagaac ctgaccttcc ccttcgagcg gacctgggat
      901 tacgacggcg acggccgcac ggacgcccgc gccaccgccc tgggcgacgg cggcgagctg
      961 cgggagttct cccggcgctt cgacggccgt ctggatacgg cggtcttggt ccggggcggc
     1021 cgggtccagc gcgtgctccg ggcgggcgtc gaactgcccc tggttccgga cgcggggggg
     1081 gccgtgctct gggtggggcg caagcccttc gacttcggaa cggtcctgcc cgagccgggg
     1141 ttcggtcggc ggggggccga gaggtaccgg atcgtgcggt tcggagacca gatcctggcg
     1201 gagagcgcgg agtgagggcc cgttccctcc cggcggccgc ccttctggcg gcggccctgt
     1261 ccgtaggctg cgcctcggat ccggcgcccg ctccggcacc ccggtacgaa agcctcaagc
     1321 tggaggcgct ggagaggctg gcgaccgagg accccgcgga gggcatggag gcagccctgc
     1381 acctccgggc cggttcctcc atcctgtccg cggatcagct ggaggactcc ctgcggaggt
     1441 ccgccgccgc cctggtccgc aggtatgagg cgagcctggc cgcgggagat ttttccgccg
     1501 cccgggcgga cgcccggtcc ctgcggttcc tgtcggagga tccccggacc gcctccgcgc
     1561 ttccggcggg tttctccctg ggcgtgtccg acgcggaact gaccttccgc atggccgaac
     1621 ggctctgggg cgaaggacgg gaaacgtccg gcctgctcgt gttcctggag gctttggacg
     1681 cggggtaccg ggacccggcc tccctccgga ccttcgcgga gcgggcccat gccctgggca
     1741 accgacaggc gctcaagagc atagagaccg tcgacccctc cgccctcacc ggagaagccc
     1801 ggacctggtc ctccggcacc gtctccatgg ccgagatgcg caggggcacg gtgaccatct
     1861 gggtcaatcg gggcatccgg atcgcccagg gcatcgggac cccggaccgg gggataggct
     1921 ccggcttctt cgtcgaccgc cggggccatc tcctcacgaa ctatcatgtc atctccagcg
     1981 aggtggatcc cgagtacgaa ggctattccc ggctctatat acgcctgccg ggccagacca
     2041 ccgaccggat acccgccaag gtgatcggct gggaccgggt gctggacctg gccctgatca
     2101 aggtggacct ggagccgccc tatgtcttct ccttcaccgg ccgccgggag ttcgtccccg
     2161 gggaccggat ctacgccatc ggttcgcccg tggggctgga caacaccctc acggcgggga
     2221 tcgtctccgc cgtcgggcgc cggttcctgc ctctgggcga ggtcatccag gtggacgccg
     2281 cggtgaaccc cgggaattcc ggaggccccc tcctggacgc ggagggggag ctcgccgggg
     2341 tcgtcttcgc cgggctgccc cggtaccagg gcctcaattt cgcggttccc gcccattggg
     2401 cggtgcgggt cctgccggac ctctaccggg gcggcgagat ccggcacccc tggctgggca
     2461 tcgcggccta cgagggccgg gaagggctgg ccgccatcta tcgccatccc gacggctccc
     2521 ctgcggctcc cgccgcgggc gaactggtcc ggatcgcggg caaggcctgc aagaccctgt
     2581 ccgaagccca ggcgatcctc ttgtcctacg atcccgggac cctggttccc gtggactatc
     2641 gggagggcgg tgaagtccgg cgctcctgga tcctcctccg gccccggccg ttttctcccg
     2701 gtgaaaccgc ggtccggcgg gacctccggg aacgcctgct cgccccgttc ttcgggatgg
     2761 ccgtcaccgc cacgggaaag aactccttcg tcgtcgagcg ggttcttccc ggtacgatcg
     2821 cggacgaggc ggggctatcg gagcgggatc ccctggtcat caaggacatc gtggtcagca
     2881 aggaagaccg cgccctcctc atgagcctct acgtcaagaa acgcaaggcg ggcttcctgg
     2941 aaagcatcat ccagatcgcc gccaccctgg acctcgattc cttcctctga ggcttgtagg
     3001 atcccgggaa tccgttatag taacaattac atcagggagg ttccgaatgg cgaaggatcc
     3061 ggccccccag gagctcgcgc cggtcggcaa gctgttcatc gacacggccg ccgaactgcg
     3121 cgccgcgctg accaaggcct tttccaagaa gggctccgtc ctgttccgat gggacggtgt
     3181 cgaggacctg gacgttcccc tgctccaact cctgtacgcc gcccgggtgg aggcttcccg
     3241 gacggggaag gagttccatt tcaccgggat cgtcacggat cgcgtggcca accgcctcta
     3301 ttcggcggga ttcgtgtcgg tcattccgct ctccggagaa gaactggaat ccaacctggt
     3361 gggattctag gaggggtcga tgctggataa gttcaagagc agcttccggg aggaagccga
     3421 ggaactcctg ggtcagctcg agtccaccct cctggagctg gaggacagcc cttccgacgc
     3481 ggagctgttg aacgccgcct tccgggccat ccacacgatc aagggttccg cgggcatgtt
     3541 cgggttcgac gagatcgccc ggttcaccca tgacctggag aacgtcctgg acaaggcgag
     3601 agccggtaag attgccatat cccgggagtt catcgatctg gccctccggg gccgggacca
     3661 catccgcggc ctcctgtctt ccggggatga actgaccgag gacctgcgcc ggaccacgga
     3721 cgccctggtg gaggaatgcc gagccctggc ggccgaaacc gaggcccgga acgaagcctc
     3781 tccgggctcc cccggggccg ccgcgccgga acagccggcg tccggagccc ccggggcggc
     3841 ttccgaaggc aagcggacct tctacatccg cttccagccg ggacccgaca tcttccggaa
     3901 cggcacccgc gtgttgtccc tcctggccga cctcgccgag ctcggggagg cgaccctggt
     3961 tccccacgtg cacgacattc cccgcctctc ggagatggat cctgagaggt gctactgcta
     4021 ctgggaaggc tacctgacca cggagtcgga ccggaacgcc atccgggacg tcttcatctt
     4081 cgtggaaagc gaatcctccc tgaccatcga ggagatcgaa gagacccgga tcaacgactc
     4141 ccggaccaag cggctggggg aactgctggt ccaatatcag cacgtaccgc ccgaggagat
     4201 ctccaaggcc ctgtcctccc agaagcggct gggggagatc ctggtcgaga agggcatcat
     4261 ctccaaggac gccctggaca cgaccctggc cggacagaac cacgccaaga agatccagga
     4321 caaggccgcc gaggccgggg gcgccctagc gtccgtgcgg gtggccagcg agaagctgga
     4381 ccagctggtg gacctggtcg gggagctggt gaccctccag gcccgcctgg cgcgaacctc
     4441 cctggacctc aaggagatct ccctggcggc gatctcggag cagttcgagc ggctgatcgc
     4501 ccagctccgg gacaacacca tgagcatacg catgctcccc atcggcagca ccttcaacaa
     4561 attccgacgg gtggtgcggg acctgtccac ggagctcggc aaggaagcgg acctcctgac
     4621 ggagggcgcc gacacggagc tggacaagac ggtgatcgag cgcctgaacg atcccctggt
     4681 gcatatcatc cggaactccc tggaccacgg catcgagtcc ccccaggacc gggaagcggc
     4741 cggaaagaac cggaagggga ccatcctcct caaggcccag cattccgggg cccacgtact
     4801 catctccgtc cgggacgacg gggccggcct caacgccgag gcgatccgcg ccaaggccgt
     4861 ggaacggggc atcgtcgccc cggggcagga gctgaccgag gcggagatca accagctgat
     4921 cttccatccc ggattcagca cggccaagtc ggtgaccaag gtttccggcc ggggcgtggg
     4981 catggacgtg gtcaagcggg agatcgattc cctgggcggg acggtgatga tatccaccga
     5041 acggggccgg ggcaccgaga tgaccctgaa gatcccgctt accctggcca tcatcgaagg
     5101 actcctggtg gccgtcgggg gcgagtacta cgtggtgccc ctgtcctctg tggacggctg
     5161 catcgagatt cccaaggagg acctgcgcaa ggtcggggag cgcaggatca tcccgtaccg
     5221 cggggacctc ttacccttcg tggacctccg gtcgtacttc gaggtccccg gcgagacgcc
     5281 cgacatcgcc cagatcgtga tcgcgaacgc ccaggattcc cgtttgggtt tcgtcgtgga
     5341 caacgtcatc ggggattacc agacggtgat caaacccctg ggcaggatgt acaaggacgt
     5401 gtccgggatt tccggcgcca ccatcctggg agacgggacc gtagcactga tcctggacgt
     5461 gaaccgccta tcccaggcgg tccaggcgga gtcccagcgc aaggtgcagg cgggttaggt
     5521 cttgaccgcc gagtccgctt ccggcgcgtc gatcgcgcat ctggcctccc tggacgagcg
     5581 ggaattccgc aggctggccg cgttcatcga gagggaactg ggcatccgga tgcccgattc
     5641 caagcgggtg atgctggaaa gccgccttca gaagaggctc cgcctgcacg gtctggccgg
     5701 cttccgggaa tacgtggact acgtcttctc cgagcaggga tcacggaccg agatgatcaa
     5761 catgatcgac gcggtcacca ccaacaagac cgatttcttc cgggaagcgg atcatttcga
     5821 ctttctccgg tcccggatcc tgccccgcat ggcggaaaac ggcgccggga gggtcctgaa
     5881 tttctggtcc gccggctgtt ccagcggcga ggagccctat accctggcca tggtgctcga
     5941 ggacttccgg tccggatcgc cccggacgga tttccgcatc ctggccacgg acatctccac
     6001 ccgggttctg gaggcggccc agagggcgat ctacgacatg gaaaaggcgg agcccatacc
     6061 ctacgaatac aagaagcgct tcctcttccg cagcaaggac ccggaccggg cggaggtccg
     6121 gatccgcccg gagctcaggt ccaaggtcga atttcgaagg gtcaatttca tggacgacac
     6181 ctatcccgtg ccctccggat tcgacacggt gttctgccgg aacgtcatca tctatttcga
     6241 ccgccccacc caggaacgga tactcggcca tatctgcgcc cacatccgcc ccgggggctg
     6301 gctcttcatc ggacactccg agaccctgac cgggatggac ctgcccctgc gcagcgccgc
     6361 tccgaccata taccagcggg aatgagaaga ggtacggaga atatggccaa gtcgatcaag
     6421 gtcatgatcg tggacgattc cgcggtcgtg cggcagaccc tgaaggaaat cctggagatg
     6481 gaccgggaga tctccgtgca ggcggtggcc tcggatccca tcttcgccct caagaagctg
     6541 gagaccgaga agccggacgt catcatctcc gacgtcgaga tgccccgcat ggacggcatc
     6601 accttcctga agaccgtcat ggagaggtac cggatacccg tggtgatctg ctccagcaag
     6661 accgaggagg gctcggacaa cgccctgaag gccatggagt acggggcgat cgagatcatc
     6721 cagaaaccca agctgggcac caagcagttc ctggaggaca gccgggtcga gatctgcgac
     6781 gtcgtccggg ccgccaacat gtcccggccc caggagcgcc gtgccccgac ccgcgtcccg
     6841 gaggtgagcc agaagctcac cgcggacgtg atgctcccca agccgggccc cgagcgcggc
     6901 gccctggaaa ccacggagag ggtcgtggtg gtgggcgcct caacgggcgg gacggaggcc
     6961 ctgaaggagt tcctggaggc cctgcccgag gactcgccgg gaatcgtgat cgtccagcac
     7021 atgccggagc acttcaccgc ggccttcgcc cgccgcctgg acggcctctg ccggcttacc
     7081 gtgaaggaag cggaggacaa cgacacggtg atccgggggc gggccctgat cgccccgggg
     7141 aaccggcaca cgcttctcaa gcggagcggg gcgcggtact atgtcgaggt gaaggacggt
     7201 cccctggtct gccgccaccg tcccagcgtg gacgtcctgt tccggtccgc cgcccggtac
     7261 gcgcaaaaga acgccgtggg ggttataatg accggcatgg gggacgacgg cgcccggggc
     7321 atgaaggagc tccacgaatc gggggcatac acgatcgccc aggacgaggc caccagcgtc
     7381 gtcttcggca tgcccaacga ggccatcaag atgggagggg tggacctggt cctccccctg
     7441 tcgaaaatat tcgaggaagt actgcgcagg acccggtagg ggccgcgtgt ccagggagga
     7501 gcgatgggca agaagattct gatcgtggat gactccacgg cgatacgcca gagcatcacc
     7561 tacatcctga accaggaagg ctacgagacc gtggaggcca aggacggcct ggaggccttc
     7621 cagatgctcg agggtctcgg gaagctggac ctcgtgatca ccgacgtgaa catgccgaac
     7681 atggacggga tcaccttcat ccggaaaacc cgggagctgg ccgcgttcaa gttcgtcccc
     7741 atcctcgtgg ccggagtgct tgttcagttc tgcggcatcg ccnnnnnnnn nngggcaagg
     7801 aggcgggggc caccggctgg atcgtcaagc ccttcagcgc ggacaagctc ctcggcgtgg
     7861 tcaagaaggt catcgactag ggggccggga tggaggaaac gtcgggccgc acgggcgagt
     7921 acctctgctt cttcctgggc caggaacagt acgcggtgaa cgtctcccgc gtggaagtcg
     7981 tgctggagat gcagaacgtg acgcgggtgc ccaaggcgcc tccccacatg cgcggcgtga
     8041 tcaaccaccg gggcagcgtg gttcccgtgg tggatctccg gatgcggttc ggcatgggac
     8101 cgtccaagct ggccgagggg acgagcatca tcatcctcca gatcgaatac ggcggggacc
     8161 tcctgaccgt gggggtgctg gccgacgggg tccgggaggt ggtcaacctg gaccccgcgg
     8221 acaccgagaa gccgccgcgc ctgggcagcc ggctggacga ggcggtgatc gagggcatcg
     8281 ccaagcgggg ggacgagttc gtcatcctcc tcaacatcga cgaggcgttc cgggaaggag
     8341 tccccgggac cgccgttccg gcgggaacgg accggtaagg gaaagtcatg gccgacggag
     8401 ccgccatcgt catcgtcgag gacgagttca tcgtcgccct ggacatccag cggtacctcg
     8461 agcgcaacgg gtactcggtc aagggcatcc tcccgtcggg ggaggagttc ctggaccggg
     8521 tcgaggatct caaaccggac ctggtcctca tggacatcaa gctgcagggc aaactcgacg
     8581 gcgtggagac cgccgccctg ctggcggacc gctgggcggt cccggtcatc ctgctcaccg
     8641 cgtacgcgga cgagccgacc ctggctcgcg ccaagatcac ccagcccttc gggtacctcc
     8701 tgaagccctt caacgagcag gagctccgga cctccatcga gatagccctg taccgatcca
     8761 tgatggaaaa gcgcctgcgg gaaagcgagc ggctcctgcg gcaggcccag aagatggagg
     8821 cggtggggcg cctggccggg ggcatcgccc acgatttcaa caacatcctg accgcgatcc
     8881 tgggctacgc caacctgctc ggggaggagg tccacggaaa ccccaagctc caggacgacg
     8941 tcgagggtgt caagaaggcg gtcaagcgcg cggaggccct gacccgtcag ctgttggcct
     9001 tctcccggcg ccagcccatg gagcccaagg tgatccagcc ccgggaggcc ctggacgaga
     9061 tgcaccggat gctggaacgg ctcctgcccg cgaacgtcca cctgatcctg cgcaacgggg
     9121 agcggcttcc ctgcatccgc atcgatccca tccacctgga gcagatcatc ctcaacctga
     9181 tcgtgaacgc ccgggacgcg atgcccgagg gcggccagat cgaggtccgc cttacgcctt
     9241 gcggattgga ttcctcccgg gcgggttcct tggagcgcat ccccgagggg tcctacgtcg
     9301 tactgacggt acaggataca ggaaccggca ttccgccctc ggattacgag aggatcttcg
     9361 agcccttctt taccaccaag gccaaggagg ggggcaccgg cctcgggctg gccacggtgt
     9421 acggaatcgt caagcaatcg aacggctaca tcgacctcga atcccgggta ggggagggca
     9481 cgtccttctc cctgtacttc ccgggggtcc agtatgaggc ggtccgggaa tcctcccggg
     9541 agcgggaggc ccgggtcgac caagggaccg aatcgatcct ggtggtggag gacgacgacg
     9601 agatccggag cctcatggag cgggccctgg cccgcagggg gtattcggtg ctgaccgcgg
     9661 cccatcccgg ggaagccatc ctggtatccg agcgccacgc cggaaggatc gacctgatcg
     9721 tggcggacat cggactgccg atcatgagcg gatcggactt ggtgaagcgc atgcggaccg
     9781 tcctgccggg agtgaaggcc ctgttcgtct cggggtaccc cgagaacgcc ccggacctgc
     9841 ccgattccgc cttcctgccc aagcccttca cggaggcggt cctggccgcc gcggtccgcc
     9901 ggctgctgga ttccccggct gcaaaaccgt gatcgatgga tctcgtttca cggattttga
     9961 caggatgaac aggatcaaga aggatcaaca ggatagggta cgaaagcgac cggatgtcct
    10021 gttgacgcat gcttcgaaat cgaaatcgga atagtatcct gttcatccgg atcccatcct
    10081 gttcatcctg tccggtttta tcctgaaaac cttgctaccc cagtatcagc cggtacagcg
    10141 cggcctccac gtcggatagg tccgcggacc cgtatctccg gcgctcccgg ggcaggagaa
    10201 gctccttgcg atccagtatc cgggcgggct tgtccgagag caccgtgact atgtccccca
    10261 ggtacagggc ctccttcacc tcgtgggtga cgaacacgac cgtccgggga tccgcggtct
    10321 gcaattccag gaaggcgtcc atgaggcccg tccgggtccg gaggtccacg gactggaagg
    10381 gctcgtccag gaggaggatg tccgcgggat aggcgaaggc ccgggccagg gagacgcgct
    10441 ggcgcatgcc cccggaaagc tgacggggcc tctggtcccg ggcctcggag agtcctacgc
    10501 cctcgaggaa ccgcagggcc cgctccctgc gctccgggac gccgaaggat gaccgcagga
    10561 caagctccac gttctccagg gccgtcaggg acggaaccag gcggggttcc tggaagacat
    10621 agctgaaccg ggcctccccg aggcctcttc gctcgccgga gtccggcggg atcaggccgg
    10681 acaggatatg gagcagggtg gtctttccgc agcccgaggg ccccaggatc gcggtgacct
    10741 tcctctccgg gaggtcaagg gaaaacccgt ccaggacggg aaggtccccg aaggctttgc
    10801 ggagttcaaa cagggagtag ggcacgggcg gccttccttc ggaggagttt gaacgcggcg
    10861 tccgtcaggg cgcagagcag gatgcccgcc agggtccagg cgaagacctc ggcggtttcc
    10921 agctggatcc gggctccctg catccccgtt cccagggccc gggcgggctg gctcaggact
    10981 tccccggcca cgaccacctt ccagcagagg cccagggcgc cgtgggacgc ggaagcgagg
    11041 tggggcgcca aggcgggcac atagatggtc cggatccggt cccgcttccg caggcggaag
    11101 agccgggcca tctccaggag gcccgggtcc gcggccccta cgccctggcg gacatccgcc
    11161 acgaccacgg gaaaggccat gaggaccgcg gcgaacaccg gtaccgcccc ggcgggaaac
    11221 cagatcaggg ccagtaggat cacggccagt accggggtcg cccggatcac cgtcatgccc
    11281 ggggaaaggg cggaggccag gaccggggac agcccgacgg ccaggcccac ggcgacgccc
    11341 gcggccatgg tgatcccgaa agcccccagg ccccgcagga aggtcccctc cagtgccgcc
    11401 aggaagcggg cggtcgggaa gagatccgcc gcggcccggg ccacccgcag gggggaggga
    11461 agtacgatct ccgaaccgat cgtccgggcg gccagttccc acagcagcag gaaacccagg
    11521 gctccggcgg ccgcggccgc cgaaccgggg atcccccgcg ccgcctcagc gcctcgggat
    11581 ttccgcatag aatcccgagt ccgggagccg gcccccgatg gatttgggct cgatgttcag
    11641 gaagaccgac agcagggcct ccacgtccgc ccgggcctcg ggggcgggta ggaagaggta
    11701 ggcggacctc gggatggcgg cggccgccac ggcggccttg agtcccaggt cgtgcttttc
    11761 caccagggct cccgcctcgg cggggttcgc cttgacccag gcgatggaat ccgcgtaggc
    11821 gccgaggagg gcccggacgg tggccgggcg gtcccggatt acagaatccc ggatcaccag
    11881 gacggacatg ggataccccg ggcgtccggt ggcctcccgc caaagctggt ccagggagaa
    11941 ggggatccgg gcccgggggt tccccgcaag ggcctgggtc gcgaaaggtt ccggcaggac
    12001 agccagggat atgcgccccg ccaccaggga agcggcgatc tccgggtagg gaagccggaa
    12061 ggcgagggcg aggtcactgt ccggatccag tccccgggct tccgcgatcg tgcggatcag
    12121 gtattccggg gtggccccct ggccggccac gtggatctcc cggccccgca ggtcctcgaa
    12181 ggaccggacc tcgggatccg tggtcaccac ggagaccatg cccatgccga ccacggcggc
    12241 caggcggtag ggcaggcccg aggcgtacag cttggcggcc aggttgacgg gcaggaccgc
    12301 cgcgtcgagc tcaccggcca ccagcttggc cgccatggcg tccgcggagg gcacggtttg
    12361 gagctccagg gaatcccccg cggcgagctt gggaggggcc tcgaaaaccc ggaccatgcc
    12421 gatgcccgtg gggcccttca gggcaccgag gcgcaggacc gttcccgccg gggcttcccg
    12481 gccgcccagg gccaaggagg gtaggacccc gagggcggcc agcaggaacg aggacatcgc
    12541 tcgagagaat cttaggttca tggaatgctc ctgctaatac gataaataaa tatccaaata
    12601 tcgtcaacgg aaaatgttcc ctcggctatc catttttcga cccgattgag tatagttaac
    12661 tggatgatat gcgatcggtg cggaaagagg gaagcctccg ttttcatacg ccgggaaggc
    12721 tcgggggagt tctccctctg cgcggaatgc gcccgggaac agggcatctc cgccgaggac
    12781 ggacgcctcc ggatctccct ggaggacctc ttctccccgc caccggagag ccccgagagg
    12841 ccgggacgcc ggacggtctg cgccacctgc gggatccggc tggaggaggt ccggaaatcc
    12901 gggcgcatcg gctgtccggc ctgctacgat tccttccggg aggaattgac cgccttcctc
    12961 cagcgaaggg gccgcaaggg accctatcga ggcgcagttc ccaggaggtt cgccgccttc
    13021 ggaaagccgt ccgccggtct ttctcccgcc gacgcgtccg ccgtaccggt cgggacggcc
    13081 ggcccggaaa cccggcacaa tcccgcggcc gtccgggatc ccgggcccgc ccgggaggat
    13141 ctgtcgggac ggcttcggga cgccttggac gcggaggatt acgagttggc cgcccggctc
    13201 agggacctcc tgcggtccct cggggaggaa gcgtcatgag cgggcccctg gacgactttc
    13261 tttccaggct tccggcgccc gccggagacg gggcggcgat caactgccga gtgcttctgc
    13321 gccggggcct cgcggagcac ctcttcccgg gacgggcgga cgaggagcag gccacccgga
    13381 cctcggaggt gctggaatcc gccctggccg gcacgggccg gttcgatatg ctcaagctgt
    13441 cccgggcgga ttccctggac cgtcgggccg cggtggaacg ggatctggtt ccccagtcct
    13501 acgcggtccg cggaggaggc ttgctggcct tcgaccggga gggctccgcc tggtgcaccg
    13561 tcaacgagac cgaccacctg cgccttcggg cggaggcctc gggggcggat ctggccgggg
    13621 cctaccgaag ggctgcggag ctggacgccc tcctggccga atccctggac tgggcgttca
    13681 gtccggacgc ggggtacctt tgcgcggaga tcgcggactg gggcacggga ctctcggcct
    13741 ccgtgacact ccacctcccg gccctggcca tggcggggct ggtggatcgc gccctccgga
    13801 cggccctgga ttccggactc tcggtgcacg gattctggtc cgacgagagc gcctcctccg
    13861 gggccctgta cgaactcgag accccccggg gagcctggac ggacgaggag tccatcctgg
    13921 tgcggctggg ggacgcggtc gaacgcgtcg gcgcctacga aaagggcgcc cgggaggagc
    13981 ttctggccca tgaccccgag accctgcggg acgccgtgtt ccgggcctac ggaacggcac
    14041 ggtacgcccg gcggatcggc tacgacgagg gggcggagac cctctcccgc ctccggttag
    14101 gggcgtgggc gggactcatc gccggtctgg agggacccaa ggcttcccgg ggtttctacg
    14161 gcttgcggaa cgcccacctg ctgaagtacc ttgcgaaggg ggacgcgccc gtcggggacg
    14221 gtcccgatac cgtacggatc gaggaagtcc gcgcccgggt tctccggacc ctggtggagg
    14281 acgcgacctt ggtggaggct ccctagcatg ttcaaagggt tgacccagcg cgcccagaaa
    14341 atactctccg tcctggccca ggaggaagcc aagcggttcc acgcggacca gttggttccg
    14401 gagcacgtgc tcctggccgt ccttcgggaa ggggagggca taggctaccg ggccctgcgg
    14461 agcctcaaga tcgacaccac ggagatccgc atcgagctcg agaagaccgc ctccggacgg
    14521 aagggcgggt tcatgctggg ggacgtgccc ctgtcccgca ggctcaagtc cctcctggag
    14581 accgccgccg aggaagccca cctggcaggc tccgagtaca tcggcaccga gcacctggtg
    14641 atcgcctccg cccgggaggg gggcagcgtg ttcgagtccc tcctgggccg ctacggggtc
    14701 tactacgagc agctccgggg ggccgtcaaa tacctgaccg cgtcccggga ggagtccgcg
    14761 gcccgggagg aacggacggc cgcgccgaaa cccgccgccc ggaccccgcc cgtagccggg
    14821 accgtccgga ctcccctgct ggacgagttc gcccgggacc tgaccgagct ggcgcgggcg
    14881 gaccgaatcg atcccgtggt cggtagggag cgggagatcc gcagggtcgt gcggatcctg
    14941 gcccggcgga ccaagaacaa tccggtcctg atcggggaac ccggggtagg caagaccgcc
    15001 atcgtggagg gcttggccct ccggatcgtc gcggggcaga tccccgacgc cctggcggaa
    15061 aaacgccttc taactctgga catggcctcg gtggtggcgg gcacgaagta ccgcgggcag
    15121 ttcgaggagc gcctcaagcg gatcatgcgg gagatcgccc aggccgggaa cgtgatcctc
    15181 ttcatcgacg agctccacac ggtcatcggg gcggggagcg cggagggaac catcgacgcc
    15241 tccaacatgc tcaagcccgc cctgtcccgc ggggagatcc agtgcatcgg ggccacgacc
    15301 ctggacgagt accgcaagta cttcgagaag gacgcggccc tggagcggag gttccagtcg
    15361 gtcctggtgg aggagcccac ggtggccgag accgtggaga tcctggccgg gatccaggag
    15421 cggtacgaac ggttccacgg ggtgacctat tcccgggacg ccgtggaggc cgccgcggtc
    15481 ctggcgcacc gctacctgac cgagcgcttc ctgccggaca aggccataga cctcctggac
    15541 gaggcggggt ccaagcggaa gatcgagaac ccctcccggc ctccggagct cccccgggtc
    15601 gaggaggaga tccagcgcct gaccgaggag aagctagccc tggtctccac ccagaactac
    15661 gagcgggccg cggagatacg ggactccatc cgcaagctca aggcccggct ggaggagatc
    15721 cgcaattcct ggggcgctga cccggggcgg gaccgtgccc tggtcacgga gagggacctg
    15781 cgggaggtga tctcggaaat caccggcatc cccctggacc ggctggagga gagcgaggcg
    15841 ggccgcctcc tgggcctgga ggaggccctg catgcccggg tcgtggggca ggaggacgcg
    15901 atccgggcgg tctccacggc ggtccggagg tcccgggcgg ggatcgcgga ccaccgtcgg
    15961 cccacgggtt ccttcatctt cctgggtccc accggggtgg gcaagaccct ggtggccaag
    16021 accctggcgg agcgactgtt cggcaccgag gaggccctga tccgggtgga catgtcggat
    16081 ttcatggaga agcacaacgc ttcccgcctc gtgggagccc ccccgggtta cgtcgggtac
    16141 gaccagggcg ggatgctgac cgagaaggtc cgccggaggc cctacagcgt catcctcttc
    16201 gacgagatcg agaaggccca ccccgacgtc ttcaacctcc tcctgcagct cctggaggag
    16261 ggggagctcc gggacaacct ggggcacacg gtctccttcc ggaactgcgt cgtcatcatg
    16321 accagcaacg cggggacccg ggacatagcc cggggcaagc ggctgggctt ccacgccgag
    16381 gatcctcggt tcgacctgga atccgtcaag gaagccgcga tgtcggaact caagcggcag
    16441 ttcaatccgg agttcctgaa ccgcgtggac gaggtcgtcg tcttccatcc cctttccaag
    16501 gaacaggcgg gagccgtcct ggacctgatg atcgcggacc taaagaagcg cctggccgag
    16561 aagggcctgg acctggagat ccgcaagccc gcccacgact ggctggtgga gaaggggtac
    16621 gacgcgacct acggggcccg gcccatgcgc cgcctgatca cccgggccct ggaggatccc
    16681 attgccgcgg acatgatcgc ggggaagtac tcccccggct ggacggtcct ggccgagctc
    16741 aagggggagg agctggtcgt ccggccccgg aagtcgccta ggccccggaa cgagccggtg
    16801 acggcccgtc gctgatcccg ggatgtgcgc gcggacctcg gctggacggg gcgggcgcgc
    16861 gctcgcggat cgcggcaccg acgaacaggc cgttggcaag cttcgagttt gtctgcgccc
    16921 tgctagccct cgtccggctc cttgccgatc aaggaacgca ggacgcggag ccggtcctcc
    16981 cgcagggtga cgtggaggtt tttttcctgg gtggggatga ccaggccttt cgggccgtcc
    17041 ttggccctgc ggaggtactt gacccgggtg tagtagaggt ccccctggcc ggagctccgg
    17101 cccttggagt agtagagggc cagggtcccc gcgtccagga ggatctccag gggcaccgac
    17161 ttcccgggcc ggttcttgac gaagacatag gaacccggcc agtcccgggc atgcagccag
    17221 aggtcgttcc cccggacatg cctgcgcagg agctcgtcgt tctccttggc cgagcggccc
    17281 accaggagag tccagccgtc ccgctccagg gcgatgccgg ggtagggacg tcgggctgcg
    17341 gccgcggccc tcctgcgggc caggaaggcg cgcagggcgt aggggctttc ctcggtctcc
    17401 aggcggacag cctcggcctc caggacctcg aggcggcgca gggtctccgc cagctcggcc
    17461 cggacgtccg ccaggcccga gcccgccttc cggtaacgct cgtagtaggc ctgcgcgttt
    17521 tccaccaggg atttcttggg gtcgatccgg atgcggatcg tcccgcctcc gtagaagtcc
    17581 ggggcctcga ggtacccgcc ctcggtggac ccggtcccga cgctgcccat caggatgtcc
    17641 ccgagctccc gcaggcgctc cgcgtccgcg tattccgtct cggtccggcg tagctcctcg
    17701 atccgggcct ccagggaggc ccgccgccgg gcgaaccatt cccggacctt ggccaggagc
    17761 tggtcccggg agagttcgcc ccggccctgg gcgtaaagcc ggtccacgcg ctcgttgaag
    17821 gtcccgggtc cgggaatgtc ccgcacctcg aagggcttct ccggggacgc cccggccggg
    17881 gcctcctcgg ggcggtagac gcctccggcg atctcccccc gcttgggctt gcgggccagg
    17941 gcgtccacga tcgagccggc atcgtccgtc aggatgaggt tggcggctcc gctccatagc
    18001 cgggcgtaca gacggtagcg gaccggatcc ggagcttccg gatccgcggg gccccggatg
    18061 tccaggcgta cgatccgctc ggcccccagc tgtcggaccg cctcgatccg tccgttgcgg
    18121 atccgggacc gcaggagctc cgcgaagcgg aggggtttct cgctcttcgg ggccgggatg
    18181 gaggtggaat ggatgcggca ggccccgtgg gccaggcaga ccagcaggtc cgtcggacag
    18241 ccgggcttgt acagggagag gaccagggtg tcgtaggccg gctggacgac gcgctggatc
    18301 tgggctccga cgaggtccag ctccgagagg acgaggtcga tctccttatg gttgaggctc
    18361 atcgtttccg gccgctccgc ggccggatgg cacccacgat cgcgccggga gtgcggccgt
    18421 cggcgatgtt tccggattgg agcttatatt cgttacacga acgcatcggg tttcctgtta
    18481 tccattcctg tggatcgcgg aacgcgaagc gttccgtgcc gtaactccga gccgcgagcg
    18541 gctcggagtt acgcgccgtg acagtgcttg tacttcttcc cgctcccgca ggggcagggg
    18601 tcgttgcggc ctaccttggg taccgtccgc ttgaccgtga ccacctgccc ggccgcggcg
    18661 gccagggttc ccgagggggc cgccgagcgg ggggccgcgg aacgggctga accgtcctgc
    18721 atgtcgcccc cgccgtggaa ctggcccatc tcgtcgtgga ccgccgttcc gggtcgggcc
    18781 gcggggacgt agcggcggtc cgtctcagtc ttgatccgga ccaggaagaa ccgggtcgcg
    18841 atggaccggc gtatgtcgtc gaccatcttt tcgaagattt cgaaaccctc gatcttgtac
    18901 tccaggaggg ggttcttctg ggcgtagtgc cgcaggtaga cggcctcccg aagggcctcc
    18961 atgttctcca ggtggtccag ccacttctgg tcgattgcct ggaggtactg gaagcggatg
    19021 aagtggttga ggttggcttc cccggcatag gcgcgcttgg cctcgaagtc cgagcgcagg
    19081 gcctccagta tgaggtcccg gagcttctcc ggctcccggg aagggggagg ggaggggagc
    19141 tggtagttga aggactcccg gagtcccgtc tggagttccg tccaggcctt gccgtcgtcg
    19201 gcgcggcggg aatccccgta ggctgccagg agttcgtccg cgatgtcctg ggc
//
LOCUS       DAOTEI010000053        19225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1882_length_19225_cov_5.487324, whole genome shotgun sequence.
ACCESSION   DAOTEI010000053 DAOTEI010000000
VERSION     DAOTEI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 19225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19225
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1882_length_19225_cov_5.487324"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="P5313_07060"
     CDS             <1..380
                     /locus_tag="P5313_07060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004792075.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRY80156.1"
                     /translation="YITEGQFYLKNGRIEPFGSLSRLKQQVNGKTRPDHRAIMDGMIK
                     LYASYKDTLEKKAMGFQMSSWDAKLLKYGELFEQEMMDLSVNIPLEGALDKGWEILAS
                     CFDPQETGLRTELAEKYWPKARA"
     gene            395..1009
                     /locus_tag="P5313_07065"
     CDS             395..1009
                     /locus_tag="P5313_07065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRY80157.1"
                     /translation="MAKIKLTKNELKKQKDALKMYKRYLPTLQLKKQQLQTEIRGIEA
                     KAKARAEERERLLAEFRAWIAVFGEEDAIRTESGDWLLSVREIRTTAGNIAGVEIPVY
                     AGADFVLADYDLYLRPLWIDKALDRLKAMLELDLEIKVLQEQIARLARELRITTQRVN
                     LFEKVKIPETAENIKRIRIYLGDQQTAQVVRGKIAKRKVVRAAS"
     gene            1018..2850
                     /locus_tag="P5313_07070"
     CDS             1018..2850
                     /locus_tag="P5313_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRY80158.1"
                     /translation="MKKAILVLRERGKSAALKSLRRLGVVHADPIEGRGQSWEAASEE
                     LARLDSAIGILLSCKSKETAPALDYQAALELARKVNETAELIKSAQERIAALRKEMER
                     IQGWGDFDPAVLAYLRENGMDLRLYEAGPKAASSMPPELPVIRVVSDKTTVRFAGFPA
                     GPVSLPEGVAEFAPPQASLSSLGAEEKASAAEAARGEAFLAEAAKSRPHLEAARERAR
                     SGLTFETLLSGMEGEGPVAWISGWVPEKDAPGLSKAAAEHGWGLLLDDPGDDEMPPTK
                     VESNPVVRMIAPVFDFLGTVPNYREYDISASFLAFFTVFFAMIFGDGGYGSILLGLGL
                     FAALKAKKAGGKVPDAVRLLLLLSSATVAWGVVTGSWFGLPAEILPGFLKGLRIGWLA
                     SDNPLSGANTKQLCFILGLVHLVWARMKNIRRDIRSLKFLAQLGLLLQLLGMYFMVLN
                     LVLDAVRFPIPNFALYLIGIGFLLNFVFASYEGNILKSILASLTNIVSVLLGVVNVFA
                     DITSYIRLWAVGLAGLAISQTVNTMAGPMLGRALLFAFGIVLIGFGHGLNLALSLLSV
                     IVHGVRLNMLEYSGHLGMEWSGFKYEPFREGSDEEASVLERSPT"
     gene            2847..3269
                     /locus_tag="P5313_07075"
     CDS             2847..3269
                     /locus_tag="P5313_07075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525271.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HRY80159.1"
                     /translation="MNIGMIGAGAVMALSAIGSGIGAGIAGQAAIGAWKRNYLANKPA
                     SFLLMIFAGAPLTQTIYGFILMGAMMNSSKDPALLLAMGLAGGAGMAMSAVAQGKASA
                     ASCDAFGETGKGFANYITIVGLCETVALFVMAFSFGAL"
     gene            3425..4459
                     /gene="ispG"
                     /locus_tag="P5313_07080"
     CDS             3425..4459
                     /gene="ispG"
                     /locus_tag="P5313_07080"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRY80160.1"
                     /translation="MRIVKVGKLLLGPGYPVVIQTMWKEPLAPGDPEPLRVRIGKLKS
                     LGCGLLRFAAPDLRSAEVLGELSRISPLPLVADIHFDWRIALRCLDFPIAKIRINPGN
                     IGSRAKVEEVVRKAADRGVPIRIGVNGGSLPQDLRGSQDPAGAMIAAAEREMEILESL
                     GFRDYLVSMKSSDMRATLDANRRFAAVHDVPLHLGVTEAGPLIPGIVKNAVALHALLS
                     EGIGVTLRVSLSDSPESEVVAGREILASAGRGRPGVNIISCPRCGRGGFDVHSFLARW
                     QDRLYALDRDITVAVMGCAVNGPEEARHADIGITGAGDRAVVFRGGKIVRTISVEEAD
                     TVFEEELSRL"
     gene            4456..5670
                     /locus_tag="P5313_07085"
     CDS             4456..5670
                     /locus_tag="P5313_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80161.1"
                     /translation="MNRTLHVRAAAVCAAVLLLAPCLGAVESRVWVEFENGKRLFREK
                     EFGQALNAFRTAATERRKMFESATRALAAALDSPQARRAEGSILALVDEYAKEIFLAS
                     ELEQINLQAGSSLRKKIDLLRQRRLTDRFRGFLDALYLTLDYRTFDDLGDSLAALQKA
                     AAALAMYPEAEFWMGRVFQAEGELKLAEIQYARAWDMREAYEVSDDRFTPLYALADLY
                     RTRGDYLRMEEQYLQIVRQDPTASDPRNEYLRNAMLNTLTRDGVDKFMSLYRTEGDFA
                     VRAFDALGSFWYESGRQGKAVLYLGLAVNRVLTKAIDAVRSEEPSYAYTTLPELLGRI
                     SARRDLEEFARNAELYRLLFSLSDALYANGARAPARELWRVLSTAPGSGTWGARSAAQ
                     LRSPVVKVPVLP"
     gene            complement(5747..6064)
                     /locus_tag="P5313_07090"
     CDS             complement(5747..6064)
                     /locus_tag="P5313_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY80162.1"
                     /translation="MEASDLCPAEKLSEYARMLKAVGHPIRLRLLCLIERQADPCVSD
                     MWKCLNQPQPVISQHLAILKQNNIVSAEVQKTRRVYSIVNPFIRDLVRDFISDASLPG
                     PKT"
     gene            complement(6089..6823)
                     /locus_tag="P5313_07095"
     CDS             complement(6089..6823)
                     /locus_tag="P5313_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY80163.1"
                     /translation="MKALLVVEDDAIADIIRFYLRPLGFDVLRYRNPLKALDNLEELE
                     PDAILVSARDFPRHWKPLVQVARRSWAKERCVFILLKGEVFPFEEAAKAMHLGVNGVV
                     KEDLGDRTELSRFQQILKRYVAIEDSRTSDRITPAAWDRLDFLFNRPSDGVPVAGRIE
                     TISMSGLSLKPDLPALAEELEPGAFLEDCSLRVGPDIVSVGCTLVRKNHVLAFAFTRI
                     AEEDRARIVAYLESAPKREIDSLLKK"
     gene            complement(6820..7755)
                     /locus_tag="P5313_07100"
     CDS             complement(6820..7755)
                     /locus_tag="P5313_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80164.1"
                     /translation="MKLPRTILVLMLAVLAAGVASAQPEIAVRFFDKRIYYTDSEIFL
                     KITIQNPSASPYRFKLAEERMFSLAFEARTPSNRLLDVSDTYKRTVASGSPVYYREIA
                     LQPGEEYSFVEDLRRYVRMDEAGVYRVRVMFHPELLRPDSRPVYSPDLVVSVRPGAGI
                     PSIAETLSAETGDILRAERIPPDEVIRRTIEARQKGRWNEFFLYLDLEALLTRTPEKK
                     RVYDKESDDGRRRMLDRYKEELEREIVDNDIVTIPYWFEILETRYSAASGIVRVLQKF
                     QYQQIRLVKEYTYQLQKRDDVWYVVGYSVLNKGTE"
     gene            7877..11308
                     /gene="mfd"
                     /locus_tag="P5313_07105"
     CDS             7877..11308
                     /gene="mfd"
                     /locus_tag="P5313_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRY80165.1"
                     /translation="MKSLYLLDVLTRLESSAPVGDFLARLADGRFPVDCGGLEGAYTA
                     LLAALCRERLGTPVFVVAPTDLEAEAFARDLGLAGVDAESFPWWGTAAYRPVSPRAPV
                     FGERSAALARALGRASGASGGDSSRPVVVVASQRAALTPVPPPDYFGGLLVALERGQS
                     FDPEAVAARLSSYGYLRVPRVSLPGEYALRGEVLDLFMPGDEEAWRVVFEFDAVEEIR
                     RFDPADQGSRPSREERVTLRPVKEVVWDEERRSVLARRLSALRGYGPENAALLAGALA
                     EFGEAKGEELLFPLAFDRPATLFDYLPRDAVVFLTERERLSGQAEAIAREYAGLYRKA
                     ALEGPVPPPETQLVDFESAAAFLPRVVRTWSLADASAEGRLAFGSEPSRSFFGNITYF
                     KEELGTLTRGGYDVRVFAETDVQAERIRLLLKDYPVQVQPAGLSAGFSVPELRLILIQ
                     ENEIFGRRKRAPKSLKTARSRVIDTFIELAPGDFVVHVNYGIGRFASIERMRVLGNDR
                     DYVKIEYAEGETVFVPIEQANLVQRYIGNEGEEPRLDRLGSKSWENRKSKVRKSVEDL
                     AERLIRIYSRRRTAKGFAFPPDGEWQTAFEASFPYEETEDQLRCIAEVKEDMESDKPM
                     DRLICGDVGYGKTEIALRAAFKAVMGGKQVAFLAPTTILAEQHYETMKERFADYPVRI
                     AMLSRLVEKKDQRRILEEARTGAVDILVGTHRLLQKDIAFKDLGLLVVDEEQRFGVKD
                     KERLKEIKASVDCLTLTATPIPRTLHMSLLKIRDMSVLTTPPSNRHPIRTFVEEYDDG
                     KIAQAVRREVEREGQVFFLHNRIESLEDVRTRLAALVPEVMVETAHGQMDARELEDVM
                     HRFIHGGFHVLVSTTIIENGIDIPNVNTIVIDRADVYGISQLYQLRGRVGRSDRVAYA
                     HLFYPDRRALSEIAMKRLQIISDFTELGSGFKIAMKDLEVRGAGNLLGREQSGDIYAV
                     GFDLYLRLLDEAVARLTQEGWEPEEDPYLELDYAGYIPDDYVTQSAVKMEMYKKIASV
                     YSPEDLEALYREFEDRFGPLPDEAASLLALAEIRLLCKRLSIASLKERGGFVTVEFSK
                     VAKVSVERLLRLMQESGQKMKLDPKRPNVLSISTGPIGLREKSEFIREKLAALAG"
     gene            complement(11318..12604)
                     /locus_tag="P5313_07110"
     CDS             complement(11318..12604)
                     /locus_tag="P5313_07110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY80166.1"
                     /translation="MNPVEPPVRSAGLRKMAVIGPAAFLNSLGIGSVNLGLIFILREV
                     FQAPPAVVGRFGALWSLVYFAACLAFRRLTARLVPRNSMTFMTASSAVIILLLCVFPS
                     LPAAFLAYSAFGAVIAFFWPPLMGWLSRGLEGRDLSRATGLFSFSWSVGGIVSPYLAG
                     TLSERDKFLPLWFAVAMFTANAVFLQMSRRFLRDSDERPVSKRAGVSPARDESSPFRF
                     SAWLSVAVLYAAVGIQANIFPLFARSELGLSESATGLLITVRMAATTAGFLFFSRFSF
                     WRFRRGFLPVPTALAGGAALILILAAKSLPVLAAGLGVFGFAAAWAYNNSMFYGASGA
                     SDRDRRMTVHEALLTAGQVFGSLLGGILYQASSMRLVFLFTALMAGAGVWAQSRMARR
                     RFGQAPAHRPPLGMERDGFSESTMEDPEIREKEERK"
     gene            12804..14708
                     /gene="ligA"
                     /locus_tag="P5313_07115"
     CDS             12804..14708
                     /gene="ligA"
                     /locus_tag="P5313_07115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRY80167.1"
                     /translation="MDKAERIRELEALVERHNDLYYNSQPEISDAEFDALRDELASLS
                     PGSPVLGRIGADAADGFPKVRHLMPMGSQDKATSPEEFLKWAAKVCHPEYLVQYKLDG
                     ASLELQYEKGILSRAVTRGDGQVGDEITPNARRMAGVLASLPEPFSGAVRGEVVMTRE
                     VHARKYADKANCRNAANGLMKRKDGIGSEDLRVICYDASSVGDALGVVDEVFFPDEKS
                     KLSWLERMGFETVPLRIFASPGEVVDWRAHVMDARPGLPYDIDGLVVKGNEIDPADMA
                     RARPEKQIAFKFSPEEAVSTVLRVEWSESGANFTPIGVLDPVRLAGTVVQRANLCNTD
                     MIRSLSLRIGSRVVITKRGEIIPKIESLVENPPDAREIPVPSDCPSCGTALVDEGTRL
                     FCPNPECPKKSLHRLEKWLSVLDVRDFGSVILGKLHAAGRVRTLADLYTLRAEELSSF
                     ERMGDVLARKILRNLAARNEVSLAEFVAGLDIEGVGELVTEKAVAAGFDTLEKLRAAT
                     PEELSAVDGFGEILARTLSEGLRALGPEIDALAASGAVRIRPPAAAGPLAGKSFCFTG
                     ELKSLKRGQAEARVKVRGGSVKSSVTKDLSYLVTNDPESGSDKNRKARAFGVPVIGEE
                     EFLALVEGTS"
     gene            14705..15304
                     /locus_tag="P5313_07120"
     CDS             14705..15304
                     /locus_tag="P5313_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80168.1"
                     /translation="MKRRGPGLFMAVLLVLALAFLGLTALTALQKAEIARLERGIQSL
                     KEELVPIRFMVLERDKGVVKARVRMYDLAGNEVDLLEMTWPGTELSFDFSVVPASGRY
                     LAFPRRIFTDRLAPSSGTNLFPRYDRAGFPGIFEGAEFGRLGRTRLSGLFQALKEDRE
                     PSGSFGSVVHDIARIAAFEPGVVYKVVVRPKGGIEILED"
     gene            15306..16211
                     /locus_tag="P5313_07125"
     CDS             15306..16211
                     /locus_tag="P5313_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80169.1"
                     /translation="MDIESLKGPLFAAADAVSFLLTRPAGWAILGGILALGFALRASG
                     ALRDRFLACEASGRRMGAGQALGILLRELYGSLAGAVAVLPTVAVVLVGAVLLVGVSD
                     SVRRLDEIAENRARISELSAVVRNLERRYRVADVRVLSADGRTARLSLAFYDSRIPGK
                     PVRTQEAAVEGSEVFVDALVLDFAYSEIESGRRVNLALPYRVYGNLQAPADGQELALL
                     DERGVPLLYRRAEAEIYGIEPEAYDARLEELMDLLEDEGARRRAGIVRSVFGAAVRRR
                     VRAGDSFTIWIEQTGGLTIKGDGGF"
     gene            16342..18111
                     /gene="argS"
                     /locus_tag="P5313_07130"
     CDS             16342..18111
                     /gene="argS"
                     /locus_tag="P5313_07130"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRY80170.1"
                     /translation="MKDAKTLWKACVSRALEALAREKGIPAEALSALPPAVEVPPKPE
                     LGDLGFPMFPYAKVLRAAPAQIAQAVAAALVGDPEAAGLGTVRTEGPYLNVFLDRPAV
                     VRDVLEALGRDPDGYGRNESLAGRKIMVEFSSPNTNKPLHLGHLRNDALGESVSRILA
                     ANGAEVFKIDLINDRGVHICKSMLAYMAYGNGRTPESEGVKSDHFVGDYYVRFNDLKK
                     EDPAAEEKAQALLVKWEAGDPDTLALWKTMNGWAEGGIRQTYERTGISFDKYYHESRI
                     YVTGKDEVLRGLESGVFFKAEDGSVRVDLSDIGLETKVLLRKDGTSIYMTQDIGLAIE
                     RHKDWPFDQLIYVVASEQQYHFKVLFHILAKLGYPWAANLYHLSYGLVNLPEGRMKTR
                     EGTVVDADTLLDSLRELAVEEIASKGREDAVGDISETAEKIALGAVHFYLLQATPSKD
                     MIFNPKESLSFNGATGPYIQYMGARISSMLRKHDSGEGNARGGTVRPELLAGDAEWAL
                     IRRIAEYPEAVAQAARDFDPSAVATCLYDLASAFSRFYHDNPVLTAPDKDTAASRLAL
                     AAAARTVLRNGLSLVCIPFLETM"
     gene            18209..18520
                     /gene="rplU"
                     /locus_tag="P5313_07135"
     CDS             18209..18520
                     /gene="rplU"
                     /locus_tag="P5313_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRY80171.1"
                     /translation="MYAVVEVKGKQYRAEKGASIRVDRMDGEKGQTVTLDSVLLVSGE
                     TVKVGAPYVSGASVKAVIADQAKADKIIVFKYKPKKDYRRKHGHRQSYTLLQVEDILG
                     A"
     gene            18523..18864
                     /locus_tag="P5313_07140"
     CDS             18523..18864
                     /locus_tag="P5313_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRY80172.1"
                     /translation="MIEVRARLGEDGVLRRVDSSGHAGTDAGSGDLVCAAASVLLRTA
                     YEALAQVPGVRVEGSAPEPGKLWFSLRDYPPGGTDRLRGIGDFLLTGLSGLEREHPGA
                     LKLTTERHWRE"
     gene            18866..19108
                     /gene="rpmA"
                     /locus_tag="P5313_07145"
     CDS             18866..19108
                     /gene="rpmA"
                     /locus_tag="P5313_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRY80173.1"
                     /translation="MAHKGVNGRDSNPHHLGIKASGNSVVTAGSIIVRQRGTHIHPGE
                     NVGIGRDDTLYALVDGRVTFSDHRGRKYAGIEPVQA"
BASE COUNT         3262 a         6284 c         6560 g         3119 t
ORIGIN      
        1 gctacatcac cgagggccag ttctacctca agaacggccg catcgagccc ttcgggtccc
       61 tgtcccggct caagcaacag gtcaacggca agacacggcc ggaccaccgg gccatcatgg
      121 acggcatgat caagctctac gcctcctaca aggacaccct ggagaagaag gccatgggct
      181 tccagatgtc cagctgggac gccaagctcc tcaagtacgg ggagctcttc gagcaggaga
      241 tgatggacct gtccgtgaac atccccctgg agggggccct ggacaagggc tgggagatcc
      301 tggcctcctg cttcgatccc caggagacgg ggctccggac ggagctggcg gagaaatact
      361 ggccgaaagc ccgggcgtga aaaggcgata gccgatggca aagatcaagc tgaccaagaa
      421 cgagctgaag aagcagaagg acgcgctcaa gatgtacaag cgctaccttc ccaccctcca
      481 gctcaagaaa cagcagctcc agaccgagat ccggggcatc gaggccaagg ccaaggcccg
      541 ggcggaggag cgggaacgcc tcctggcgga gttccgcgcc tggatcgcgg tcttcgggga
      601 ggaggacgcc atccgcacgg aatccgggga ctggctcctc tcggtccggg agatccggac
      661 caccgcgggg aacatcgcgg gcgtggagat ccccgtgtac gcgggcgccg acttcgtgtt
      721 ggccgactac gacctctacc tccggccgct gtggatcgac aaggccctgg accggctcaa
      781 ggccatgctc gagctggact tggagatcaa ggtcctccag gagcagatcg cccggctggc
      841 ccgggagctc cgcatcacca cccagcgggt taacctattc gagaaggtga agatcccgga
      901 gaccgcggag aacatcaaac ggatccgcat ctacctgggg gaccagcaga ccgcccaggt
      961 ggtccgcggc aagatcgcca agcggaaggt agtgagggcg gcgtcatgat agcacccatg
     1021 aagaaggcca tcctcgtcct gagggagcgc ggcaagtccg cggccctcaa gtccctgcgc
     1081 cgcctggggg ttgtgcacgc ggatccgatc gagggacggg gacagagctg ggaagcggcc
     1141 tcggaggagc ttgcccgact cgattccgcg atcggcatcc tgctgtcctg taagtccaag
     1201 gaaaccgcgc cggccctcga ctaccaggcg gccctggagc tggcccggaa ggtgaacgag
     1261 acggcggagc tcatcaagtc cgcccaggag aggatcgccg cccttcgcaa ggagatggag
     1321 cggatacagg gctgggggga cttcgatccc gcggtcctgg cgtacctgag ggaaaacggc
     1381 atggaccttc ggctctacga agccggcccg aaggcggctt cctcgatgcc gccggagctt
     1441 ccggtgatcc gagtcgtttc cgacaagacc acggtgcggt tcgcgggatt cccggccggg
     1501 ccggtgagcc tgcccgaggg ggttgccgaa ttcgccccgc cccaggcctc cctgtcctcc
     1561 ctcggtgccg aagagaaagc gtcggcggcc gaggcggcac ggggagaggc ttttctggcg
     1621 gaggccgcca aatcccggcc gcacctggag gccgcccggg aacgcgcccg gtccggactc
     1681 accttcgaaa ccctactgtc gggaatggaa ggggagggtc ccgtcgcctg gatctccggc
     1741 tgggtcccgg aaaaagacgc tcccgggctc tcgaaggccg cggccgaaca cggctggggt
     1801 cttctcctgg acgaccccgg ggacgacgag atgcccccca ccaaggtgga gagcaacccc
     1861 gtcgtccgca tgatcgcccc ggtcttcgac ttcctgggga cggtcccgaa ctaccgggag
     1921 tacgacatct ccgcctcctt cctggccttc ttcacggtct tcttcgcgat gatcttcggg
     1981 gacggcggct acgggagcat cctcctgggc cttgggcttt tcgcggccct gaaggccaag
     2041 aaggccggag gaaaggttcc ggacgcggtg aggctgctcc tgcttctgtc ctccgccacc
     2101 gtggcctggg gcgtcgtcac ggggagctgg ttcggacttc ccgccgagat cctgccgggc
     2161 tttctgaagg gcctccggat aggatggctc gcctcggaca accccctgtc cggagccaac
     2221 accaaacagc tctgcttcat cctgggcctc gtgcatctcg tctgggctcg catgaaaaac
     2281 atccggcggg atatcaggtc cttgaaattc ctcgcccaac tggggctcct gctccagctc
     2341 ctggggatgt acttcatggt cttgaacctg gtcctggacg ccgtccggtt cccgattccg
     2401 aacttcgccc tctacctgat cgggatcggg ttcctgctca acttcgtgtt cgccagctac
     2461 gaggggaaca tcctgaagag catcctggcc agcctgacga acatcgtatc ggtcctgctg
     2521 ggggtggtga acgtcttcgc ggacatcacc tcctacatcc gcctctgggc cgtgggcctt
     2581 gccggactgg cgatcagcca gaccgtcaat actatggccg gccccatgct gggccgggcc
     2641 ctgctgttcg ccttcggaat cgttctgatc gggttcggcc acggcctgaa cctcgcgctg
     2701 agcctcctgt ccgtcatcgt gcacggcgtg cggctcaaca tgctggaata ttccggccat
     2761 ctcgggatgg agtggtcggg gttcaaatac gagcccttcc gggaagggtc ggacgaagaa
     2821 gcttccgtac ttgaaaggag tcctacatga atatcggtat gatcggggcc ggtgcggtca
     2881 tggcgttatc cgccataggt tcgggtatcg gggcgggcat cgcgggccag gcggccatcg
     2941 gggcgtggaa gaggaactac ctggccaaca agccggcctc cttcctcctc atgatcttcg
     3001 cgggcgcccc cctcacccag accatctacg gcttcatcct catgggagcc atgatgaact
     3061 cttccaagga cccggccctc ctcctggcca tgggcctggc cggcggcgcc ggcatggcca
     3121 tgtccgccgt agcccaggga aaggcctccg ccgcctcctg cgacgccttc ggggagaccg
     3181 ggaagggctt cgcgaactac atcaccatcg tcggcctctg cgagaccgtg gccctcttcg
     3241 tgatggcctt cagcttcggg gcgctgtaag agaaagtgaa taggtgagtc gtgaatgggt
     3301 gaatgggata gggtgggggg gatgaggtcc gacctgcttg aacatggatc gggtcccgaa
     3361 tcgcagaccg accatccggc cccattcgct attcacccat tcacctattc actgatctga
     3421 ctccatgcgc atcgtcaagg tcggcaagct cctcctcgga ccggggtatc ccgtggtcat
     3481 ccagaccatg tggaaggagc ccctggctcc cggggatccg gaacccctcc gcgtccggat
     3541 cgggaagctg aaatccctgg gatgcggcct gctccgcttc gcggctccgg atctacggtc
     3601 cgcggaggtc ctcggggagc tgtcccgtat ttccccgctt cccttggtcg cggacatcca
     3661 cttcgactgg aggatagccc tccgctgtct cgactttccc atcgccaaga tccgcatcaa
     3721 tccgggcaac atcggctccc gggccaaggt ggaggaggtg gtccgcaagg ccgcggaccg
     3781 gggcgttccc atccggatcg gcgtgaacgg tggatccctg ccccaggacc tccggggctc
     3841 gcaggatccc gcgggtgcca tgatcgccgc cgcggagcgg gagatggaga tcctggaatc
     3901 cctgggcttc cgggactacc tcgtgtccat gaagtccagc gacatgcggg ctactttgga
     3961 cgcgaatcgc cgcttcgcgg cggtccacga cgtccccctg cacctgggcg tcaccgaggc
     4021 gggccccctg atccccggca tcgtcaagaa cgccgtggcc ctccacgcct tgctctccga
     4081 ggggatcggg gtgactctac gggtctccct gtccgacagc ccggaaagcg aggtcgtcgc
     4141 cggccgggag atcctggctt ccgcggggcg gggccggccg ggagtcaaca tcatctcctg
     4201 cccacgctgc ggccggggag gcttcgacgt ccacagtttc ctggcccgat ggcaggaccg
     4261 gctctacgcc ctggaccggg acataaccgt cgccgtcatg ggctgcgccg tcaacgggcc
     4321 cgaggaggcc cgtcacgcgg acatcggcat cacgggcgcc ggggaccggg ccgtcgtgtt
     4381 ccgggggggc aagatagtcc gaacgatttc cgtggaagag gccgatactg tattcgagga
     4441 agaactgtcg cgcctatgaa cagaaccctg catgtccgag ccgcggccgt ctgcgccgcc
     4501 gtgctcctcc ttgccccctg cctgggcgcc gtggagtcca gggtgtgggt ggaattcgag
     4561 aacgggaagc ggctgttccg ggagaaggag ttcgggcagg ccctcaacgc cttccggacc
     4621 gcggccaccg agaggcggaa gatgttcgaa tccgccaccc gcgccctggc cgcggccctg
     4681 gattcgcccc aggcccgccg ggcggagggc tccatcctgg ccctggtgga cgagtacgcc
     4741 aaggagatct tcctggcctc ggagctcgag cagatcaacc tccaggcggg atcctccctc
     4801 cggaagaaga tcgacctcct gcggcagcgc aggctgaccg accggttccg gggtttcctg
     4861 gacgcacttt acctgaccct ggattaccgc accttcgacg acctggggga ttccctggcc
     4921 gccctgcaga aggcggcggc cgccctggcc atgtaccccg aggcggagtt ctggatgggc
     4981 cgggtgttcc aggcggaggg ggagctcaag ctcgcggaga tccagtatgc ccgggcctgg
     5041 gacatgcggg aggcctacga ggtctccgac gaccggttca cccccctgta tgccctggcg
     5101 gacctgtata ggacccgggg ggactacctg cggatggagg aacaatacct ccagatcgtt
     5161 cggcaggatc ccacggcctc ggatccgagg aacgagtacc tgcgaaacgc catgctgaat
     5221 accctcaccc gggacggagt ggacaagttc atgagcctgt accgcaccga gggggatttc
     5281 gcggtacggg ccttcgacgc cctggggagt ttctggtatg aaagcggaag gcagggcaag
     5341 gcggtcctct acctcggact ggccgtgaac cgggtcctga cgaaggccat cgacgccgtc
     5401 cgctccgagg agccctccta cgcctacacg acgctgccgg aactcctggg tcggatctcg
     5461 gcccggaggg acctggagga attcgcccgg aacgcggaac tctaccggct gttgttctcc
     5521 ctttcggacg ccctgtacgc caacggggcc cgggctccgg cccgggagct ctggcgtgtc
     5581 ctgtcgaccg cgcccggctc ggggacctgg ggcgcccgtt ccgcggccca gctccggtct
     5641 ccggtcgtca aggttcctgt ccttccctga gtcctgtccc gagccgaggg ggcgtatcgc
     5701 taccggggtg ggggaggggg tttctcgaag atgcgagtag gaaaactcag gtcttgggac
     5761 cgggaaggct cgcgtccgaa atgaagtccc gaaccaggtc ccggatgaag ggattcacga
     5821 tggaatagac acgccgggtc ttctggactt cggcggatac gatgttgttc tgcttgagga
     5881 tggccaggtg ctgggagatg accggctggg gctggttcag gcacttccac atgtcggata
     5941 cgcagggatc ggcctggcgt tcgatcaggc agaggagccg cagacggatg ggatgtccca
     6001 cggccttgag catccgcgcg tactcggaga gcttttccgc ggggcacaga tcgctggctt
     6061 ccatggtttc cattgtataa taaaatcttt atttcttcaa tagggagtct atttctcgtt
     6121 tcggggcgga ttccaggtac gccacgatgc gggcccggtc ctcctcggcg atccgggtga
     6181 aggcgaaggc caggacgtgg ttcttgcgga ccagggtgca ccccacggaa acgatgtcgg
     6241 ggccgacccg cagggagcag tcttccagga acgcccccgg ctcaagttcc tccgccaggg
     6301 cgggaaggtc cggcttgagg gacagacccg acatgcttat cgtctcgatc ctgccggcca
     6361 cgggtacgcc gtcggacggg cggttgaaca ggaagtccag ccggtcccag gccgcggggg
     6421 tgatgcggtc cgaggtgcgg gaatcctcga tggccacgta gcgtttcagg atctgctgga
     6481 accgggaaag ctcggtccgg tcccccaggt cttccttgac gaccccgttc acccccaggt
     6541 gcatggcctt ggccgcctcc tcgaagggga agacctcgcc cttgaggagg atgaagacgc
     6601 accgttcctt ggcccaggac cggcgggcca cctggaccag gggcttccag tgccggggaa
     6661 agtcccgggc gctgaccagg atggcgtcgg gctccagctc ctccaggttg tccagggctt
     6721 tcaggggatt ccggtaccgg aggacgtcga agcccaaggg acggaggtaa aagcgtatga
     6781 tgtccgcgat ggcgtcgtcc tcgacgacca ggagcgcctt cattcggtcc ccttgttcag
     6841 caccgagtag ccgacgacgt accagacgtc gtcccgcttc tgcagctggt aggtgtattc
     6901 cttgaccagg cggatctgct ggtactggaa tttttgcagg acccgcacga tgccgctggc
     6961 ggcggagtac cgggtttcca ggatctcgaa ccagtagggg atcgtcacga tgtcgttgtc
     7021 cacgatctcc cgctccagct cctccttgta ccggtccagc atcctccggc ggccgtcgtc
     7081 ggattccttg tcgtagaccc gtttcttctc gggggtccgg gtgagcaggg cttccaggtc
     7141 gaggtagagg aagaactcgt tccagcgccc cttctggcgg gcctcgatgg tcctgcggat
     7201 cacctcgtcc gggggtatcc gttcggcccg caggatgtcc cccgtctcgg cggagagggt
     7261 ttccgcgatc gaaggaatcc ccgccccggg ccgcacggaa accacgaggt ccggggagta
     7321 cacgggacgg gagtccggtc tcaggagttc cgggtggaac atcacccgga cgcggtagac
     7381 ccccgcttcg tccatgcgta cgtagcggcg gaggtcctcc acgaaggagt actcctcccc
     7441 gggctggagg gctatctccc ggtagtagac cgggcttccc gaggcgacgg tgcgtttgta
     7501 ggtgtcgctg acgtccagga ggcggttgga gggggtgcgg gcctcgaagg ccagggagaa
     7561 catcctctcc tccgccagct tgaaccggta aggcgatgcc gaagggttct ggatggtgat
     7621 cttgaggaag atctcggaat ccgtgtagta gatgcgcttg tcgaagaagc ggacggcgat
     7681 ttccggttgt gcggaggcga cgccggcggc caggaccgcg agcatcagga ccagtatcgt
     7741 gcgcggaagc ttcaccgggt acgctccttg taagtcccta tcgtattgta tatcatgggc
     7801 cgccgtccag gattccgacg ccgttttcgg acggagttcg gctcaagatc tcccgtatga
     7861 ttctcggaaa ccttgcatga aatcattata cctgctggac gtactgaccc gtctggaatc
     7921 ctccgctccc gtgggggatt tcctcgcccg gctggcggac ggccgcttcc ccgtggactg
     7981 cggcggcctg gaaggcgcct acacggctct cctggcggcc ctctgccgcg aacgactcgg
     8041 caccccggtc ttcgtggtcg cgccaacgga cctggaggcg gaggccttcg cccgggacct
     8101 cgggctggcg ggggtggatg ccgagagttt cccctggtgg ggcaccgccg cctaccgccc
     8161 cgtctccccc cgggccccgg tcttcgggga gcggagcgcc gccctggccc gggccctggg
     8221 ccgggcttcc ggcgcctccg ggggggattc ctcccgcccc gtcgtcgtgg tggccagcca
     8281 acgggccgcc ctcacccccg tgcccccccc ggactacttc ggaggcctcc tcgtggccct
     8341 cgagcgggga cagagcttcg atcccgaggc ggtcgcggcg cgcctgtcct cctacggtta
     8401 cctgcgggtc ccccgggtct cccttcccgg ggagtacgcc ctgaggggcg aggtcctgga
     8461 cctcttcatg cccggggacg aggaggcctg gcgggtggtc ttcgagttcg acgcggtgga
     8521 ggagatccgg cgattcgacc ccgccgacca gggctcccgg ccttcccggg aggagcgtgt
     8581 gacgctccgc ccggtcaagg aggtggtctg ggacgaggag cgcaggtccg tcctggcccg
     8641 tcggctgtcc gccctgcggg gctacggtcc cgagaacgcc gctctccttg ccggggccct
     8701 ggcggagttc ggggaggcca agggcgagga gctcctcttt cccctggcct tcgaccgtcc
     8761 cgccaccctc ttcgactacc tgccccggga cgcggtcgtc ttcctgacgg agcgcgagcg
     8821 gctgtcgggc caggccgagg cgatcgcccg ggagtacgcg ggactgtacc gcaaggccgc
     8881 cctggaggga cccgttcccc cgccggagac ccagctggtc gacttcgagt ccgcggcggc
     8941 cttcctccct cgggtcgtgc gcacctggag tctggcggac gcctccgcgg aaggccgcct
     9001 ggccttcggt tccgagccct cccggtcctt tttcgggaac atcacctact tcaaggagga
     9061 gctgggaacc ctgacccggg gcgggtacga cgtccgggtc ttcgcggaga ccgacgtaca
     9121 ggcggagcgg atccgccttt tgctcaagga ttaccccgtc caggtccagc ccgccggcct
     9181 ttccgcgggg ttctcggtcc ccgagctccg cctgatcctg atccaggaaa acgagatctt
     9241 cgggcgtcgg aaacgagccc ccaagtccct gaagaccgcc cggtcccggg tcatcgacac
     9301 cttcatcgag cttgcacccg gggacttcgt ggtccacgtg aactacggga tcggccgctt
     9361 cgcctccatc gagcggatgc gggtcctggg caacgaccgg gactacgtga agatcgagta
     9421 cgccgagggc gagacggtct tcgtgcccat cgagcaggcc aacctggtcc aacggtacat
     9481 cggcaacgag ggcgaggaac cccggctgga ccgcctgggc tccaagagct gggagaaccg
     9541 gaagtccaag gtgcgcaagt ccgtggagga cctggcggag cgcctgatcc ggatctactc
     9601 ccggcgccgc accgccaagg gcttcgcatt tcccccggac ggggagtggc agaccgcctt
     9661 cgaggcttcc ttcccctacg aggagaccga ggaccagctc cgctgcatcg ccgaggtcaa
     9721 ggaggacatg gagtccgaca agcccatgga ccgcctgatc tgcggagacg tgggctacgg
     9781 caagaccgag atagccctgc gggccgcctt caaggccgtg atgggaggca agcaggtcgc
     9841 cttcctggcc cccacgacca tcctggcgga acagcactac gagaccatga aggagagatt
     9901 tgccgactat cccgtccgta tcgccatgct ctcccggctg gtggagaaaa aggaccagag
     9961 gcggatcctg gaggaggccc ggacgggggc cgtggacatc ctggtgggaa cccaccggct
    10021 tcttcagaaa gacatcgcct tcaaggacct gggactcctg gtggtggacg aggagcagcg
    10081 cttcggggtc aaggacaagg agcgcctaaa ggagatcaag gcctccgtgg actgcctgac
    10141 cctgacggcc acccccatcc cgaggaccct gcacatgtcc ctcctcaaga tccgggacat
    10201 gtccgtgctg accacgccgc cctccaaccg tcaccccatc cggaccttcg tggaggagta
    10261 cgacgacggg aagatagccc aggcggtgcg gcgggaggtg gagagggagg ggcaggtctt
    10321 cttcctgcac aaccgcatag agagcctgga ggatgtgagg acacgcttgg ccgccctcgt
    10381 gccggaggtg atggtggaga ccgcccacgg gcagatggac gcccgggagc tggaggacgt
    10441 gatgcaccgg ttcatccacg ggggcttcca cgtcctggta tccaccacca tcatcgagaa
    10501 cgggatcgac atccccaacg tcaacaccat cgtcatcgat cgggcggacg tctacggcat
    10561 ctcccagctc taccagctgc gcggacgggt gggccgctcg gaccgcgtgg cctacgccca
    10621 ccttttctac ccggaccgca gggcgctctc ggagatcgcc atgaagcgcc tgcagatcat
    10681 ctcggacttc acggagctgg ggtcggggtt caagatcgcc atgaaggacc tggaggtccg
    10741 cggggcgggc aacctcctcg ggcgggagca atccggggac atctacgccg tggggttcga
    10801 cctctacctc cgtctcctgg acgaggccgt ggcccggctc acccaggagg gctgggagcc
    10861 cgaggaggac ccctacctcg agctggacta cgcgggctac attcccgacg actacgtcac
    10921 ccagagcgcg gtcaagatgg agatgtacaa gaagatcgcc tcggtctact ccccggagga
    10981 cctggaagcc ctgtaccggg agttcgagga ccgcttcggg cccctgccgg acgaggccgc
    11041 gagcctactg gccctggcgg agatccgtct tctctgcaag cgcctgtcca tcgcgtccct
    11101 gaaggagcgg ggcggcttcg tgaccgtgga gttctccaag gtggccaagg tttccgtgga
    11161 gcgcctgctc cggctgatgc aggagagcgg ccagaagatg aaactggatc ccaagcggcc
    11221 caacgtcctg tccatatcca cgggcccgat cggcctccgg gagaagagcg agtttattag
    11281 agaaaaactc gccgccctgg ccggatgatc ggtgccccta tttcctttct tctttctccc
    11341 tgatctccgg gtcctccatg gtcgattccg aaaaaccgtc tcgttccatg cccaagggcg
    11401 gccggtgggc gggcgcctgg ccgaagcgcc gtcgggccat ccgggattgc gcccataccc
    11461 ccgccccggc catgagggcg gtgaacagga acaccagcct catggaggat gcctggtaga
    11521 ggatgccgcc cagcagggac ccgaagacct gccccgcggt gagcagggcc tcgtgcaccg
    11581 tcatccgcct gtcccggtcg gacgccccgg aggccccgta gaacatggag ttgttgtagg
    11641 cccaggcggc cgcgaaaccg aagaccccga gccccgcggc caggacgggc agggacttcg
    11701 cggccaggat caggatcagg gccgccccgc ccgccagggc cgtggggacc ggcaggaacc
    11761 ccctacggaa acgccagaag gagaatcggc tgaagaacag gaagcccgcg gtcgtggcgg
    11821 ccatccgtac cgtgatcagg agccccgtgg cggattccga caacccgagt tccgatcgcg
    11881 cgaagagcgg gaagatgttg gcctggatgc cgacggcggc gtacaggacg gcgacggaga
    11941 gccaggcgga aaacctgaag ggcgagcttt cgtcccgggc gggagagact ccggcgcgct
    12001 tcgaaaccgg ccgctcgtcc gagtcccgga ggaagcgtcg ggacatctgc aggaagactg
    12061 cgttcgcggt gaacatcgcg acggcgaacc agaggggaag gaatttgtcc cgctcgctca
    12121 gggtccccgc gaggtagggg ctgacgatcc cccccacgct ccaggaaaag ctgaacaggc
    12181 cggtcgcccg ggagaggtcc cggccttcca ggccccggga cagccagccc atgaggggag
    12241 gccagaagaa ggcgatgacc gcgccgaagg cggagtaggc caggaaggcc gcgggtaggc
    12301 tgggaaagac gcagaggagc aggatgatga ccgccgacga ggcggtcatg aaggtcatgg
    12361 aattccgggg aaccagccgc gccgtcagac gccggaaggc caggcaggcc gcgaagtaga
    12421 ccagggacca gagggccccg aaccgtccca ccaccgccgg gggggcctgg aagacctccc
    12481 gcaggatgaa gatgaggccg aggttcacgc tgccgattcc caggctgttc aggaacgcgg
    12541 cgggccctat gacggccatc ttccgcagcc ccgcggatcg gaccggaggt tcgacgggat
    12601 tcatgcggat ccagctcctt gatgcgtcgg tcggggcgcg gcctgccgcg ccccgtgggc
    12661 gagaaggcag actaggccgc ggcggcgggt taggtcaagg cggttttgac atccggctcc
    12721 cgggggctgt acaatcgatc ccggcggggg acgccggccg gagcggccgg gaaccccggc
    12781 ccggaacgga gggacggagc cggatggaca aggcagagcg gattcgggaa ctggaagccc
    12841 tggtcgagcg tcacaacgac ctgtactaca actcccagcc cgagatctcc gacgcggagt
    12901 tcgacgcgct ccgggacgag ctcgcctccc tttccccggg gagtcccgtc ctgggaagaa
    12961 tcggggcgga tgccgccgac ggattcccca aggtccgcca cctcatgccc atgggcagcc
    13021 aggacaaggc cacgagcccc gaggaatttc tcaagtgggc cgccaaggtg tgccatcccg
    13081 agtacctggt gcagtacaag ctggacgggg cctccctgga gctccagtac gagaagggaa
    13141 tcctctcccg ggcggtaacc cggggggacg gacaggtcgg ggacgagatc accccgaacg
    13201 cccggcgcat ggccggggtc ctggccagcc tgccggaacc gttttccggc gccgtgcggg
    13261 gcgaggtggt gatgacccgg gaggtccacg cccggaagta cgcggacaag gcgaactgcc
    13321 ggaacgcggc caacggcctc atgaagagga aggacgggat cgggagcgag gatctccggg
    13381 tgatctgcta cgatgcatca agcgtcgggg acgcccttgg cgtcgtggac gaagtttttt
    13441 ttccggacga gaagtccaag ctgtcctggc tggagcggat gggtttcgag accgtgcccc
    13501 tgcggatctt cgcctccccc ggggaggtgg tggattggcg ggcccacgtg atggacgccc
    13561 gcccggggct tccctacgac atcgacggcc tcgtggtgaa gggcaacgag atcgaccccg
    13621 cggacatggc ccgggcccgg ccggaaaagc agatcgcctt caagttctcc cccgaggagg
    13681 ccgtgtcgac ggtcctgcgg gtggagtgga gcgagtcggg ggcgaacttc acccccatcg
    13741 gggtcctgga ccccgtgcgc ctggccggga cggtcgtcca gcgggccaac ctctgcaata
    13801 ccgacatgat ccgctccctg tcgctccgga tcgggagccg ggtggtcatc accaagcggg
    13861 gggaaatcat ccccaagatc gaatccctgg tggagaatcc gccggacgcg cgggagatcc
    13921 cggtaccctc ggactgcccc tcctgcggta ccgccctggt ggacgagggg acacgcctgt
    13981 tctgcccgaa cccggaatgt ccgaagaagt ccctgcatcg cctggagaaa tggctctcgg
    14041 tcctggatgt acgggacttc ggctcggtga ttctgggcaa gctccatgcc gccggccggg
    14101 tccggaccct ggcggacctc tacaccctgc gggcggagga gctctcgtcc ttcgagcgca
    14161 tgggggacgt cctggcccgg aagatcctgc gcaacctggc ggcccggaac gaggttagcc
    14221 tggcggaatt cgtggcgggc ctggacatcg agggggtggg ggagctggtg acggagaagg
    14281 ccgtggccgc cggcttcgac accctggaga agcttagagc cgccacgccg gaagaacttt
    14341 cggccgtcga cggcttcggg gagatcctgg cccgtaccct gtcggagggg ctccgcgccc
    14401 tgggcccgga gatcgacgcc ctggccgcct ccggggccgt ccggatccgc ccgcccgccg
    14461 cggcgggtcc cctggccggg aagagcttct gcttcaccgg ggagctcaag tctctgaagc
    14521 ggggccaggc ggaagcccgg gtgaaggtcc gggggggctc ggtcaagtcc tcggtgacca
    14581 aggatctgtc ctacctggtg acgaacgatc cggagtccgg atcggacaag aaccgcaagg
    14641 cccgggcctt cggagtcccg gtgatcggcg aggaggaatt cctcgccctg gtggagggaa
    14701 catcgtgaaa cgtcgaggtc ccggcctgtt catggccgtc cttctagtgc tcgccctggc
    14761 cttcctgggc ctgaccgccc taacggccct gcagaaggcg gaaatcgccc ggctcgagag
    14821 ggggatccag tccctcaagg aggagctcgt cccgatccgg ttcatggtcc tggagcggga
    14881 caagggggtg gtgaaggccc gggtccggat gtacgacctg gccgggaacg aggtggacct
    14941 cctggagatg acctggccgg gaacggagct ctccttcgat ttctccgtcg tgcccgcctc
    15001 gggccgctac ctggccttcc cccgccggat cttcacggac cggctggccc cctcctcggg
    15061 gaccaacctc tttccccggt acgaccgggc cgggtttccc gggatcttcg agggggcgga
    15121 gttcgggcgg ctcgggagaa cgcgcctttc ggggctcttc caggccctca aggaggaccg
    15181 ggaaccctcg ggctccttcg ggagcgtcgt gcacgacata gcccgcatcg cggccttcga
    15241 gccgggggtg gtctacaagg tggtcgtccg gcccaagggc gggatcgaga tcctggagga
    15301 ctgaggtgga catcgaatcc ctcaagggcc cgctcttcgc ggccgccgac gccgtgagct
    15361 tcctcctgac ccgcccggcg ggctgggcga tcctgggcgg gatcctggcg ctcggattcg
    15421 cccttcgggc ctccggggcc ctgcgagacc gcttcctggc ctgcgaggcc tcgggccggc
    15481 ggatgggcgc ggggcaggcc ctcggcatcc tgctccggga gctgtacggg agcctcgcgg
    15541 gtgccgtcgc tgtactgccc acggtcgcgg tcgtgctggt cggagccgtc ctcctggtgg
    15601 gggtctcgga ttccgtgcgg aggctcgacg agatcgccga gaaccgggcc cgcatctccg
    15661 agctctccgc ggttgtgcgg aacctggagc ggcgctatcg ggtggcggac gtccgggtcc
    15721 tgtccgcgga cggccggacc gcccgtttgt ccctggcctt ctacgactcc cggataccgg
    15781 gcaagccggt ccggacccag gaggcggcgg tcgagggctc cgaggtcttc gtggacgccc
    15841 tggtcctcga cttcgcctac tccgagatcg agtccggccg gagggtgaac ctggccctcc
    15901 cgtaccgggt ctacggcaac ctccaggccc cggcggacgg ccaggagctg gccctcctgg
    15961 acgaacgggg cgtcccactc ctgtaccgcc gggcggaagc ggagatctac ggcatcgaac
    16021 cggaggccta cgacgcgcgc ctggaggaac tcatggacct cctcgaggac gagggggccc
    16081 ggaggagggc cgggatcgtc cggagcgtct tcggggccgc ggtccggcgc agggtccggg
    16141 ccggggattc cttcacgatc tggatcgagc agaccggggg cctcacgatc aagggggacg
    16201 gcgggttctg aacgggcagc cgatccgccg cccggggctg tccgaaaagc tcagtagctt
    16261 tttggacagc ccgaccgtat tgcccttcct ccccgcgccg ctatagaatc ccggcgacca
    16321 caccatcccc gaggagatcc catgaaggac gcgaaaactc tctggaaggc ctgcgtatcc
    16381 cgagccttgg aggcgctcgc ccgggagaaa gggatccccg cggaggcgct ctcggccctg
    16441 cccccggccg tcgaggtgcc gcccaagccg gaactcgggg acctcgggtt ccccatgttc
    16501 ccctacgcca aggtcctccg ggccgcaccg gcccagatcg cccaggccgt ggccgccgcc
    16561 cttgtcggag atcccgaggc cgcgggcctg gggaccgtcc gcacggaggg cccctacctg
    16621 aacgtcttcc tggaccgtcc cgccgtggtg cgggatgtcc tggaggccct cggccgggat
    16681 cccgacgggt acggccggaa cgagtccctg gcgggtcgga agatcatggt ggagttctcc
    16741 agccccaaca cgaacaagcc cctccacctg ggccacctcc ggaacgacgc cctgggggag
    16801 agcgtgtccc ggatcctggc ggccaacggg gcggaggtct tcaaaatcga cctgatcaac
    16861 gaccggggag tccacatctg caagtccatg ctggcctaca tggcctacgg gaacggccgt
    16921 acccccgagt ccgagggggt caagagcgat catttcgtgg gggactacta cgtccggttc
    16981 aacgacctca agaaggagga tcccgccgcg gaggagaagg cccaggccct cctcgtgaaa
    17041 tgggaggccg gggatccgga caccctggcc ctgtggaaga ccatgaacgg gtgggccgag
    17101 ggcgggatcc ggcagaccta cgagcggacc gggatctcct tcgacaagta ctaccacgag
    17161 agccggatct acgtgaccgg gaaggacgag gtcctccggg gcctggaatc cggggtcttc
    17221 ttcaaggccg aggacggctc cgtacgggtg gacctttcgg acatcggcct ggagaccaag
    17281 gtcctgctcc gcaaggacgg gacttccata tacatgaccc aggacatcgg cctggccatc
    17341 gaacggcaca aggactggcc cttcgaccag ctgatctacg tggtggccag cgaacagcag
    17401 taccatttca aggtcctctt ccacatcctg gccaagctgg gctacccctg ggccgccaac
    17461 ctctaccatc tttcctacgg tctggtgaac ctgcccgagg gtcggatgaa gacccgggag
    17521 ggcaccgtgg tggacgcgga caccctcctg gactccctcc gggaactggc tgtggaggag
    17581 atcgcctcca agggccggga ggacgcggtg ggtgacatct cggagaccgc ggagaagatc
    17641 gccctggggg ccgtgcactt ctacctcctc caggcgacgc cctccaagga catgatcttc
    17701 aaccccaagg aatccctctc gttcaacggg gccacgggtc cctacatcca gtacatgggc
    17761 gcccggatct catccatgct ccggaaacac gactccgggg aggggaacgc ccggggcggg
    17821 accgtccggc ccgaactcct ggcgggggac gcggaatggg ccttgatccg caggatcgca
    17881 gagtacccgg aggccgtggc ccaggccgcc cgggacttcg acccctcggc ggtggcgacc
    17941 tgtctgtacg acctggcctc ggcgttcagc cggttctacc acgacaatcc cgtcctgacc
    18001 gcgccggaca aggatacggc cgcgtcccgc ctggccctgg ccgcggcggc ccggacggtc
    18061 ctgcggaacg ggctttccct ggtgtgcata cccttcctgg agacgatgtg acggctacaa
    18121 ttgacatgag gcgccgttcc atcgtataaa gtcacgctcg cgggaagtgt ttaccgagcc
    18181 gtaccatacg aacatagagg ttatccagat gtacgcagtc gtcgaagtga agggcaagca
    18241 gtaccgggcg gaaaagggcg ccagcatccg cgtggatcgc atggacggcg agaagggcca
    18301 gacggtcacc ctcgattccg tcctcctagt gtccggcgag accgtgaagg tcggcgcgcc
    18361 ctacgtttcc ggcgcctcgg tcaaggccgt gatcgcggac caggccaagg ccgacaagat
    18421 catcgtcttc aagtacaagc ccaagaagga ctaccgccgg aagcacggac accggcagag
    18481 ctacaccctg ctccaggtcg aggatatcct cggggcctag ggatgatcga ggtccgggcc
    18541 cggttggggg aggacggcgt gctccggcgc gtcgattcct ccggccacgc gggaacggat
    18601 gccggcagcg gggacctcgt ctgcgccgcg gcctccgtcc tgctgcggac cgcctacgag
    18661 gccctggccc aggtccccgg ggtccgggtc gagggatcgg cccccgagcc ggggaagctt
    18721 tggttctccc tgcgggacta cccgccggga ggcacggatc gattgagggg tatcggggat
    18781 ttcctgctga ccgggctgtc cggcctggag cgggagcacc cgggtgcctt gaagctgaca
    18841 acggaacggc attggaggga atgatatggc tcacaagggc gtgaacggcc gggactcgaa
    18901 cccccaccat ctcggcatca aggccagcgg gaactccgtc gtgaccgcgg gctccatcat
    18961 cgtgcggcag aggggcaccc acatccaccc cggcgagaac gtcgggatcg gccgggacga
    19021 caccctgtac gccctggtgg acggacgggt gaccttctcg gatcaccgcg gccgcaagta
    19081 cgccggaatc gagcccgtcc aggcctaggg acgggaacgc ggcccggcgg cgcccctagg
    19141 gcgcccgcgg gattcgactg gagccgcccg cggggataga gccgcgggcg gttttttttc
    19201 taccaggggc gccggacggg atgta
//
LOCUS       DAOTEI010000054        19140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1897_length_19140_cov_4.779094, whole genome shotgun sequence.
ACCESSION   DAOTEI010000054 DAOTEI010000000
VERSION     DAOTEI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 19140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19140
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_19140_cov_4.779094"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="P5313_07150"
     CDS             <1..734
                     /locus_tag="P5313_07150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY80174.1"
                     /translation="GTHLDGEIHFYTPGKDIASLSMDFLVHEGAVVDLSDCAQDYDVY
                     TSKQVEERVEVKEGDILIIHTGFHHYGWDQPTGDEIRYMIKHPGPDREFAEWAKRKKL
                     RWIGVDCGSADHPMNTKIRDWMPKQAAECDKHFKQKYGKGLDQVFSDDKYQLMHIEMF
                     SEHVIHAECVGGDIDLLLNQRCTIGCFPWRFVDGESSISRIVAMVEDDRYEKLMAKKA
                     KCELTRFGDIAGAKAAWLHEEARKR"
     gene            810..2003
                     /locus_tag="P5313_07155"
     CDS             810..2003
                     /locus_tag="P5313_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRY80175.1"
                     /translation="MPGPLEGVRVLDLTRVLAGPFCTMMLADLGAEVVKVEVPGIGDD
                     SRGFGPFKNGKSLYYLTVNRGKKSVALDLKKDEGKDLLLKLARKSDILVENFRPGTME
                     KLGLGWEVLKKENPRLIYAAVSGFGHSGPDSRKPAYDILVQARGGVMSITGWPNSPPT
                     RIGMSMGDITAAVFGSSGILAALYQREKTGKGQKVDVAMLDCQLAILENALVRYQVEG
                     KAPSPLGTRHPTITPFQAFRASDAWFVVAVGNDSLWKTFCETLDRADLLADPRFATNG
                     DRTKHIADLIPELERVFARRKAQEWIDLLEGAGVPSAPVNSVDKVMQDPQLRARNMLV
                     TVEDPEVGTVTIPGNPIKMENLPESPTRPPASGIGQDTEEVLSGLLGLSPAQIRKLRE
                     SGALG"
     gene            2000..3307
                     /locus_tag="P5313_07160"
     CDS             2000..3307
                     /locus_tag="P5313_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY80176.1"
                     /translation="MNLFRPLDAEGDARAFRSGARDPADRIREVCDRIDRSEPEVRAL
                     LPEPGRRERLLAEARALAARYPDPSERPPLYGLLVGVKDIFNAEGFETRAGSRLPPDE
                     LGGPESEALKALKAAGALVLGKTVTTEFAFFAPGPTRNPRDLGRTPGGSSSGSAAGAA
                     AGFFHLALGTQTIGSISRPAAYCGVAGWKPSYGRVSAAGVFPFSPSVDHIGLLAPDAR
                     ALALGAAAVCGGWKGLPASASRPVLAVPEGPYLDQAGLEARERFERTVADLAGAGWKV
                     LRLPALRDIGEINARHRRIAAAEMDRTHARLFPKYEALYAPATRDLILEGRKVSDTEL
                     AADREGRTALRRVLEEDLAAAGADLWISPSAPGPAPEGLGSTGSPALNLPWTHAGLPT
                     LSLPAGTFLNGLPAGLQIAAPFGRDEDLLRFGIELEPYLNSDS"
     gene            3353..4765
                     /locus_tag="P5313_07165"
     CDS             3353..4765
                     /locus_tag="P5313_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HRY80177.1"
                     /translation="MNFSWKIVIDAGAISMALLAATLIRSRVKFFQRFMIPNALTAGF
                     LLLPLYNYVFPGLGHATNRLGELVYHLLNISFVAMTLRAAPPQIHKKSGGVVGTTTGI
                     LSQYAIQALTGLLLAYLLIVTVSPKLNPAIGLFLPLGFALGPGQAFAIGKGWEPMGFE
                     GAGSLGLTFAALGYLWACFAGVWLINYGVKKGWLHKEALQAFQDKGMMTGIVPKDRER
                     PVGARLTTDSEAVDSFSFHAGMVAGTYFLSFLLFKVLTWGLSLAGNAGRELAVNLWGI
                     NFIFSALTAQLVRFLMIRLKVTHVLDDSTLSRMSGFAVDYMVAGAIAAISLVFVGQYW
                     MEILLLSTVCGILTTITVPWMASRMFKDYRFERMMMIYGCSTGTLSTGLALLRVLDPE
                     FRTPVTSDYMVSAGLTFLLAIPFILAINLPAKAGQTGDMMPFWIMVILSGIYLAVTLG
                     MYVFLARRRTLAQAGKIWHP"
     gene            complement(4715..5971)
                     /locus_tag="P5313_07170"
     CDS             complement(4715..5971)
                     /locus_tag="P5313_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY80178.1"
                     /translation="MPAAVNKTYSPGNPRWFNRLLHWTYGVYLRLTYRIRIVNADIFQ
                     TLRPPYVIVPNHVCMVDPFMVGSNVPVPVHWIASDGNMRTRIMRALLRLVGTIPKSKH
                     IPDLQTINGIVEVVRKRKGVVGIFPEGTASFDGHTQALVPATGKLLKLLKVPVVTPVL
                     KGAFYSLPRWSWTRRRGALEIEFKVALTTEDIKRLSPDEILSRVEASLEHDEAEWLAA
                     RGVPFSGRRKRAEHLETVLFQCPSCGEVGRMRSSEDRFLCESCGQASRLDRYYRFRPA
                     EGRPPRFSTIREWSLWQEEAFRDYLKERVSKDDGKPIFSDPGVLVLKGWRLSPLRRIR
                     IGTLSMYRDRIELAPLTGRPLAFPLAEVEGEGVLKRNLLEFYRGRSVYQVRFPTRRSS
                     ARQWMMAISILRVPDLSGLGQGSAAG"
     gene            complement(6324..7202)
                     /locus_tag="P5313_07175"
     CDS             complement(6324..7202)
                     /locus_tag="P5313_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRY80179.1"
                     /translation="MNSEPSGTRAARAAAVLAILAFTGMVAVNGLASSLPLNGVSTGQ
                     LSDELPNLFVPAGITFSIWGLIYLLLAGYVAAAARETFRSAEGVRRAWTGRDGALFIL
                     NAAANTAWILAWHWRLVGTSLVIMLVILGTLVYLERDTERKLLPGGVLAPGPGTESGS
                     IRLRRFLLTAPLRVYLGWICVALIANATALLVKTGWGGFGLDPRVWTVGVILAGLAVA
                     LGFTIWKRQVAAPLVVVWAYAGIVLKRLQTDPAYSAPVWIAAALAALVILASMVRVRV
                     ACPLFGRKTAGKSLKK"
     gene            7300..8301
                     /locus_tag="P5313_07180"
     CDS             7300..8301
                     /locus_tag="P5313_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY80180.1"
                     /translation="MNLDASETRDAASRAQGLLARAREGMARRLVGKSELVDGILTAV
                     VADGHVLLEGVPGLAKTLAVKTFAELAGLTFRRVQFTPDLLPGDITGSLFYDAPTGRF
                     VPREGPVFTNVLLADEVNRAPAKVQSALLEAMEERQVTIGDRSIPLPKPFFVLATQNP
                     IEQEGTFRLPEAQVDRFLLKVLADYPDMEEELEIVRRVADGMSAAHKPLLSVEDIEFL
                     RSAARSVRVEPDILDYAVRLVRASRPTGDDGAPQALRELRRYMEYGASPRASIYLYRC
                     ARGGALFAGRDFALPEDVKAAALPVLRHRIIPSYEAEAEGITSDVVIRRLLSIVPVP"
     gene            8302..9225
                     /locus_tag="P5313_07185"
     CDS             8302..9225
                     /locus_tag="P5313_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY80181.1"
                     /translation="MPEEAARRTLRRLLFTAPVLSRALLSGGFRSVFRGRGIEFDALR
                     DYDAEDDARQIDGRATARFGRAFVRTYREDRNLTVLLVLDESQSMSYGTDRTRREAAG
                     IAASLLAYAAALNGTFVGALFFDTRGVGFVPPRAGRRPALSLTERLAVGEAGARGPAA
                     VRPTAGRSAGLAGALEAARAVLKRRSLVFVLSDFRAAADGPAGYSGPLGRLAARHDVV
                     AVRVTDPADTAPPGSGAASEIFDAESGRPVFLVPGSKGLREAWSAFQGGMRLDCLRAL
                     RASGAAFLELGTGDDPGKALTAFFERRRRGR"
     gene            9222..10100
                     /locus_tag="P5313_07190"
     CDS             9222..10100
                     /locus_tag="P5313_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80182.1"
                     /translation="MRLSAAVLLCLSLTAAGAQSRVQVSGTTVTPARPYVGDLVECSV
                     TFDPGTARIPEGVLDLPPRAGTAEETELRSAEVRRKAGSWVYTARFVAWVPGPARVPV
                     PASANFLLPEIRVEIASALDDFGRSPPAYKDPLELPGTRLLVWGASGGLLAGAILAWS
                     VAFGLLPWIRRLRRAWKDGRAGRDFGRSLDYLESAAAEFRPEELWALLSKSLREYLAA
                     RSRLPYQAFTAREARAASPEALPPEAAEEAADLLAEGEAVRFARSDRGAGIPRALERS
                     REILRSVEEAARDLLR"
     gene            10084..11082
                     /locus_tag="P5313_07195"
     CDS             10084..11082
                     /locus_tag="P5313_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY80183.1"
                     /translation="MTSFDNPLAFLLVLALPGWLFLRARALRRGGSLRFPLDEAAGRR
                     FEGPSGFRKAASRGALVLGILAWLGLVVAAAGPQSVERRTVFPNRGDDVMFALDLSPS
                     MGARDVEPDRLSAAKTLLELFLREDRADSVGIVGFGREAALLCPPTTDYAALRERLAR
                     AKPGALGDGTALGLGLAQAIRHAARARGTTDRVILVTDGENNAGSISPEDACDLAARL
                     GVRLTIIGVGSRGDVPLAYEDPRTGERLEGTYASGFDESALRTLAARAGGEYLSARDG
                     PALERAFRAVSGRMPGAEGARSVTVRRSRAASVLAVSVVALALSWLLGLAARGWLS"
     gene            11079..12632
                     /locus_tag="P5313_07200"
     CDS             11079..12632
                     /locus_tag="P5313_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY80184.1"
                     /translation="MTFLRPEALWGLVLLAPYAVLEALSVLRGGALAGRFAPGAEAGP
                     SRRAWIRRRAGKAVAGAWAWAFAVFALAGPSWGFRPTAGAPSGVEAALVLDVSNSMLS
                     PDLPPSRLEAARGLARALVRARPEVQYSVTAFKGGAVLLCPRTDSADALDQALEWAGP
                     SVLTTRGTSAGEGLRTALEGFSPDPGRIRWIVLFSDGNDLGAAAAPALREIRERGARL
                     FAVGCGGVDPSPVSDETGAPVLLPDGSPARTALRADTLRAWVREGDGLYVDLADPGAL
                     RILSDALDSRPGSPGARPARRPADRTGTAAFLALLGAAARALLGLPFLPGRRRGPRTA
                     AALLALTILTGCSGPRLRVLEGNRRLRRGQYEDAIAAYLAAGEKAGEGIVALDLATAY
                     SRMGEGAAADPLFALAQSSRLSEVAAAAYHNQGVRLFEASRFDEAAQAFMNALRLVPQ
                     DLETKRALELARAAAASSRLSRASRRQASAIGEGGRDEALLSLLGRAESGWFRPPASP
                     SADSGGLDH"
     gene            12629..14377
                     /locus_tag="P5313_07205"
     CDS             12629..14377
                     /locus_tag="P5313_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80185.1"
                     /translation="MSGPARRRILSAVLLGVARILAAQEVTYSVTIAPEPPTAGEEFR
                     VSVLLPGASAREVRAAEPAASGDLAFVSLAVSSAVIDSPEGSRPGARVVYAFRPLRSG
                     DLRIPRLEVSVAGSPLPLGPLALEVRPSGAAPPPALYEWKAPAAVIRWQSFSARLEPR
                     APSAPALGDYGLPATKGLSLEPAGGSAFVGIALEEGELFLPPAASREGGRVSEGARVR
                     SLPAPRELAASRAVGDFTLRLIRVGAGPVRAGQTLTVRAEVRGRGNLPILDPPEIRIR
                     APGGGPISPPETVPISEIRIAAGAYEGMAARELRFTPEVPGDYVVESGPFPFLNPDTG
                     ELRTLRASPLRISVDPPPREARPRTELRTLLDARIAAFADSGSPWSAAASRARDGDVE
                     AALHLLPVTSAPEASHLKGILLMAEEDGAAALAALGAAERRSRWLPGLAETLELCETT
                     FGVGPRIRDRLPQPRIFGALSVVFAAAGLSILGLFRLRARSAGGTPGAIGRALVSAAF
                     LWLLLCGASALERRVSYAVAASPDSFTVPSESGTPGGSFQGRAGIVGAVSSDWTLLRF
                     PDGRSAWFRTGDIHRY"
     gene            14386..14892
                     /locus_tag="P5313_07210"
     CDS             14386..14892
                     /locus_tag="P5313_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRY80186.1"
                     /translation="MADFGKILDEWDRRSRRDFRRPRRPDPEEVEEGKSRRDPAREAL
                     ERAMLAGVDPSRKGEEGRDPSPLSRAEIEALPVEAVLDLHGLTSEAAWEALSRFFRDA
                     SSRGLTKVLVIHGKGNHSEGEPVLKRTALRFLETSSLAGRHGTAERRLGGSGAVWVLV
                     RKGAQRSR"
     gene            complement(14877..15095)
                     /gene="infA"
                     /locus_tag="P5313_07215"
     CDS             complement(14877..15095)
                     /gene="infA"
                     /locus_tag="P5313_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRY80187.1"
                     /translation="MAKEEAIEVEGKVKESLPNTMFRVELPTGHVILAHLSGKMRKHY
                     IRIVPGDTVKVALSPYDLNRGRIIYRER"
     gene            15293..15649
                     /locus_tag="P5313_07220"
     CDS             15293..15649
                     /locus_tag="P5313_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HRY80188.1"
                     /translation="MEVDFVEEMKESLLRQKREILDNLIATNADFRAIVEEMDPKDFA
                     DVASDDIDRKMIEALGSQDIKRLRAIDSALARIQLGKYGLCVKCSKKIPRERLEAIPY
                     AVLCIECQKSEERRNR"
     gene            complement(15829..16671)
                     /locus_tag="P5313_07225"
     CDS             complement(15829..16671)
                     /locus_tag="P5313_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRY80189.1"
                     /translation="MLATYHAHTTYCDGRDTPDAMADAAFAAGYRVLGFSAHAPVPVP
                     SPGNLSPDRVAPYAAAVRAAAARYKGRMEILTGLEIEWVPGLGIPPEDGYAAVPIDYR
                     IGSLHFARLSGDSFFSVDGPAESFREGVRAGYGGDGTLLYRDYYRELGGLVRTGGFQI
                     LGHLDIVKMHNKDGRWFDEGSREYLSAAFEVAEALTGTRIAVEVNTGGPARGKTSEPY
                     PSLPILRELRRRGIPIVIGADAHTAAHLDPHWRRIGLDLAREAGYRELAVLGRNGWTT
                     GPLE"
     gene            complement(16759..19128)
                     /locus_tag="P5313_07230"
     CDS             complement(16759..19128)
                     /locus_tag="P5313_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY80190.1"
                     /translation="MREPRPGAPFRETFEQYPVSAALTDGEGRLVFWNRAFEDTFRSL
                     AGQAPEALEGSLFDWLELRGSFKVSHYVRELVAGRQASAQLESPVSSADRGKLWFRIR
                     LSRVDAPGGSGGVYILAAFEDDTERKRRELQLLAAKEEAEKATQTKSIFLANMSHEIR
                     TPIQTILGMTELLGDTRLDKEQMDYVRTVRFSADILLGLINDILDFSKIEAGKMELER
                     TEFDLRSVVSQAGDLLILDAHKKGLEVILDTEERLPRTLRGDPGRFRQIIVNLLKNAI
                     KHTPSGEIVVSLSRRDGERRAFLRCDVADSGTGVPESVRPRLFTPFTQAVSSLTRGGT
                     GLGLAISRHFSELMGGRIGYLPGKDGGSVFWFEVPLEPGADDRPLAYPPFVPTPRVLC
                     VDDHPRARDFLLRVLASFGMETRAAASGEEALAILREAGRTGIPFDLCLVDQEMPGMD
                     GWRLASEIKSDPELDSVRLLLMIPEGGRGAEAKMKLLQWFEADVSKPVNPEELHEVLD
                     KALTEVPELEAVEDGAPAREERRVGEGRSVLLAEDHAVNQELFTLLLERLGCRVTVAS
                     DGVEAVERGTGTSFDVVLMDIFMPRMNGYEAAQTLRAQGYRGPIVAVTASALKGERDK
                     CLASGMDDILTKPFKKADLEALLARILPPQPDGSAPDDEGTPAPGAGAGGTDAEILDL
                     EEAVATFLGNRPTVLDLLGRFLRKAESQLQELAEAESAEDWQKARELAHSIKGAAWNL
                     SARRLGNAAKAVEDAGRDGRGGDVRAGLPVLREALAELKAAVGSVNGSK"
BASE COUNT         2765 a         6683 c         6615 g         3077 t
ORIGIN      
        1 taggcacgca cctggacggg gagatccact tctacacccc cggcaaggac atcgccagcc
       61 tctccatgga cttcctcgtg cacgaggggg cggtcgtgga cctctcggac tgcgcccagg
      121 actacgacgt ctacacctcc aagcaggtgg aggagcgggt ggaggtcaag gagggggaca
      181 tcctgatcat ccacacgggc ttccaccact acggctggga ccagcccacg ggggacgaga
      241 ttcgctatat gatcaagcat cccggaccgg accgggagtt cgcggagtgg gccaagcgca
      301 agaagctccg ctggatcggc gtggactgcg gcagcgcgga ccaccccatg aacacgaaga
      361 tccgggactg gatgcccaag caggccgcgg agtgcgacaa gcacttcaag cagaagtacg
      421 gcaagggcct ggaccaggtc ttctcggacg acaagtacca gctcatgcac atcgagatgt
      481 tcagcgagca cgtcatccac gcggagtgcg tgggcgggga catcgacctc ctgctgaacc
      541 agcgctgcac catcggctgc ttcccctggc gcttcgtgga cggggagtcc agcatttccc
      601 gtatcgtggc catggtggag gacgaccggt acgagaaact gatggccaag aaggccaagt
      661 gtgagctgac ccgcttcggc gacatcgccg gggccaaggc cgcttggctc cacgaggaag
      721 cccgcaagcg ttgacgcggc gctccggtcc gccccgcggc cttcggaccc ggggcgcccg
      781 gcgcccgaga cattccgcaa ggaggcacta tgcccggacc gctggagggc gtgcgcgtcc
      841 tcgacctgac ccgcgtgctc gcgggtccct tctgcacgat gatgctggcc gacctggggg
      901 ccgaggtcgt caaggtggag gttcccggga tcggggacga ctcccggggc ttcggcccct
      961 tcaagaacgg aaagagcctg tactatctca ccgtgaaccg ggggaagaag agcgtcgccc
     1021 tggacctgaa gaaggacgag ggcaaggacc tcctcctcaa actggcccgg aagtcggaca
     1081 tcctcgtgga aaatttccgc ccgggaacca tggaaaagct gggcctcggc tgggaggtgc
     1141 tgaaaaagga gaacccccgg ctgatctacg ccgccgtctc cggattcggc cattccggcc
     1201 cggactcccg caaacccgcc tacgacatcc tggtccaggc ccggggcggg gtcatgagca
     1261 tcaccggctg gcccaactcc ccgcccaccc ggatcgggat gtccatgggg gacatcacgg
     1321 cggccgtctt cggctcctcc gggatcctgg cggctctgta ccagcgggaa aagaccggga
     1381 agggccagaa ggtggacgtg gccatgctgg actgccagct ggccatactg gagaacgccc
     1441 tggtccggta ccaggtggag ggaaaggccc cttcccccct gggtacccgg cacccgacca
     1501 tcaccccctt ccaggccttc cgggcctcgg acgcctggtt cgtggtggcc gtgggcaacg
     1561 attccctctg gaagaccttt tgcgaaaccc tggaccgggc ggaccttctg gcggaccccc
     1621 ggttcgccac gaacggggac cggaccaaac acatcgccga cctcatcccc gagctcgaga
     1681 gggtcttcgc gcggcgcaag gcccaggagt ggatcgacct ccttgagggg gccggggtgc
     1741 cgagcgcccc ggtcaattcc gtggacaagg tgatgcagga tccccagctc cgggcccgca
     1801 acatgctcgt gaccgtggag gatccggagg tcggcaccgt gacgatcccg ggcaacccca
     1861 tcaagatgga gaaccttccg gaatccccga cccggccccc cgcgtccggg atcggccagg
     1921 acaccgagga ggtcctttcg ggcctgctcg ggctatcccc tgcccagatc aggaagctgc
     1981 gcgagtcggg ggccctgggt tgaacctctt ccggcccctg gacgccgagg gggacgcccg
     2041 ggccttccgc tcgggcgccc gggaccccgc ggaccggatc cgggaggtct gcgaccggat
     2101 cgaccggtcg gaacccgagg tccgggccct cctgccggaa ccgggccgcc gggaacggct
     2161 tctggccgag gcgcgggccc tggccgcccg atatccggac ccctcggagc ggccccccct
     2221 gtacggcctc ctggtggggg tcaaggacat cttcaacgcc gagggtttcg agacccgggc
     2281 gggctcccgg cttcctcccg atgagctcgg ggggccggag tccgaggccc tcaaagccct
     2341 caaggccgcg ggcgccctgg tcctgggcaa gaccgtcacc accgagttcg cctttttcgc
     2401 ccccggcccg acccggaacc cccgggacct cgggcgcacc cccggggggt ccagctccgg
     2461 gagcgccgcg ggcgccgcgg cgggattctt ccacctggcc ctgggcaccc agaccatcgg
     2521 ctccatctcc cgtcccgccg cgtactgcgg ggtcgcgggc tggaagccct cctacggccg
     2581 ggtcagcgcc gccggggtct ttcccttctc gccctcggtg gaccacatcg gccttttagc
     2641 gccggacgcc cgggccctgg ctctcggcgc cgccgctgtc tgcggcggat ggaaagggct
     2701 tccggcttcg gcatccaggc ccgtcctggc ggtgcccgag ggaccctacc tcgatcaggc
     2761 cggcctcgag gcccgggaac gcttcgagcg caccgtcgcg gacctggccg gggcgggctg
     2821 gaaggtccta cgccttccgg ccctccggga catcggggag atcaacgccc ggcaccgccg
     2881 catcgccgcg gccgagatgg accgcaccca tgcccggctc ttcccgaagt acgaggccct
     2941 gtacgccccc gccacccggg acctgatcct ggaggggcgg aaggtctcgg ataccgagct
     3001 tgccgcggac cgggaagggc ggacggccct ccggcgggtg ctggaagagg acttggccgc
     3061 agcgggcgcg gacctgtgga tctcgccctc cgcccccggg cccgcgccgg aaggccttgg
     3121 gtccacgggc agccccgccc tgaacctgcc ctggacccac gcggggctgc cgaccctgtc
     3181 cctccccgcg ggaaccttcc tgaacggctt gcccgcgggt cttcagatcg cggccccctt
     3241 cggccgggac gaggacctgc tccgattcgg gatagaattg gaaccatacc taaattccga
     3301 tagctgaaga gcgaaccccc gagaacggac ggcttagatt aagggaggaa gcatgaattt
     3361 ttcttggaag atcgtcatcg acgcgggtgc catttccatg gctctcctgg ccgcaaccct
     3421 catccggtcc cgggtgaagt ttttccagcg tttcatgatc ccgaacgccc tgaccgcggg
     3481 gttcctgctg ttgcccctgt acaactacgt cttcccgggc ctggggcacg cgacgaaccg
     3541 cctcggggag ctggtctacc acctgctgaa catctccttc gtcgcgatga ccctccgcgc
     3601 ggcccccccc cagatccaca agaagtccgg cggagtcgtg ggcaccacga cgggcatctt
     3661 gtcccagtac gcgatccagg ccctgacggg cctcctgttg gcgtacctcc tgatcgtgac
     3721 cgtatccccg aagttgaacc ccgccatcgg cctcttcctg cccctgggat tcgccctggg
     3781 gccgggacag gccttcgcca tcggaaaggg ctgggaacct atgggattcg agggggcggg
     3841 atccctgggc ctcaccttcg ccgccctggg gtacctctgg gcctgcttcg ccggggtctg
     3901 gctcatcaac tacggggtca agaagggctg gctccacaag gaggccctcc aggccttcca
     3961 ggataagggg atgatgaccg gcatcgtccc gaaggaccgg gagcggcccg tgggggcgcg
     4021 gctcaccacg gactcggagg cggtggattc cttcagcttc cacgcgggta tggtggcggg
     4081 aacctacttc ctgtccttcc tgctcttcaa ggtcctgacc tggggactct cgctcgcggg
     4141 gaacgcgggc cgtgagctgg ccgtgaacct ctggggcatc aacttcatct tctccgccct
     4201 caccgcccag ctggtccggt tcctgatgat caggctcaag gtgacccacg tgctggacga
     4261 ctccaccctg tcccgcatgt ccggcttcgc ggtggattac atggtggcgg gagccatcgc
     4321 ggccatttcc ctggtcttcg tgggacagta ctggatggag atcctcctcc tgtccacggt
     4381 ctgcgggatc ctcaccacga tcacggtgcc ctggatggcc tcccggatgt tcaaggatta
     4441 ccggttcgag cgtatgatga tgatctacgg ctgttccacg ggcaccctgt ccacgggtct
     4501 cgccctcctc cgggtcctgg atccggagtt ccggaccccg gtcacctcgg actacatggt
     4561 ctccgcgggg ctcaccttcc tcctggccat ccccttcatc cttgccatca accttcccgc
     4621 caaggccggc cagaccgggg acatgatgcc cttctggatc atggtcattc tgagcggaat
     4681 ctacctggcg gtcaccctgg ggatgtacgt cttcctagcc cgccgcagaa ccctggccca
     4741 ggccggaaag atctggcacc cgtaggatcg agatcgccat catccactgc cgggcggagg
     4801 acctgcgggt ggggaaccgg acctggtaca cggaccggcc ccggtagaac tccagcaggt
     4861 tgcgcttgag gacgccctcg ccttccacct ccgcaagggg gaaggcgagg ggcctgcccg
     4921 taagaggcgc aagctcgatc cggtcccggt acatcgagag ggtgccgatc cggatccgcc
     4981 gcagggggga gagcctccac cccttgagca cgaggacccc gggatccgag aagatcggct
     5041 tgccgtcgtc cttggacacc ctctccttca ggtaatctcg aaaggcttcc tcctgccaca
     5101 ggctccattc ccgtatggtc gagaaccgcg gcggcctgcc ctccgccggc cggaaccggt
     5161 agtaccggtc caggcggctg gcctgtccgc aggactcgca gaggaagcgg tcctcggagc
     5221 tccgcatccg tcccacctcc ccgcaggagg gacactggaa caggacggtc tccaggtgtt
     5281 ccgcccgttt cctccggccc gagaagggga ccccccgggc ggccagccac tccgcctcgt
     5341 cgtgctccag ggaggcttcc acccgggaca ggatctcgtc cggggacagc cgcttgatgt
     5401 cctccgtggt caaggccacc ttgaactcga tctccagggc gccccggcgc cgggtccagc
     5461 tccatcgggg cagggaatag aaggccccct tcagcaccgg ggttaccacc ggcaccttga
     5521 ggagcttgag gagcttgccc gtggcgggaa ccagtgcctg ggtgtggccg tcgaagctgg
     5581 ccgtcccctc cgggaagatg cccaccaccc ccttgcgctt acggacgacc tccacgatgc
     5641 cgttgatggt ctgcaggtcc gggatgtgct tggacttggg gatggtgccc accaggcgca
     5701 ggagggcccg catgatccgg gtgcgcatgt tcccgtcgct ggcgatccag tggaccggga
     5761 ccgggacgtt ggagccgacc atgaagggat ccaccatgca tacgtggttc ggaacgatca
     5821 cgtagggcgg ccggagggtc tggaagatat cggcgttgac gatccggatc cggtacgtaa
     5881 ggcgcaggta gaccccgtag gtccagtgga ggaggcggtt gaaccagcgg ggattgccgg
     5941 gcgaatacgt cttgtttacg gcggcgggca tggtcggccc ctcgggatgt acaggatagg
     6001 ccgtatccac ggcgcggtat aacgatacct caggatcccc ggccgcgcaa ggagggaaaa
     6061 tcgggaaagg ttcttaagct gcgccgatcc ccctgcggga ccgaagacat ggaggcgaag
     6121 gatcacccgg agattgaacc acggagacac agagtcacag agcgcgaggc tgacgtacgt
     6181 cagcctcgcg gcgacgaaga ccttcgatct tcgtcgatgt tgccggagtt gggatcgccg
     6241 aagcttgctt cggcgccgac cggccggcgc tgccggccgg tcgctctctg tgtctctgtg
     6301 gttttgtttg tattgagatc gcttcatttc ttcaaggatt tcccggctgt ctttcgcccg
     6361 aacagggggc aggcgacccg gacccgcacc atggaagcca ggatgaccag ggccgccagg
     6421 gccgcggcta tccagaccgg ggccgagtag gccggatccg tctggagccg cttgaggacg
     6481 atgcccgcat aggcccagac cacgaccagg ggagcagcga cctggcgttt ccagatcgtg
     6541 aagcccaggg ccacggccag gccggccagg atgaccccca cggtccagac cctcgggtcc
     6601 agcccgaagc cgccccaccc ggtcttgacc aggagggccg tggcgttggc gatgagggcc
     6661 acgcagatcc atccgaggta gacccggagg ggggcggtca ggaggaagcg ccgcaggcgg
     6721 atcgatccgg actccgtccc ggggcccggc gcaaggacgc ctccgggcag gagcttacgc
     6781 tccgtgtccc gctccaagta gacgagggtc ccgaggatga ccagcatgat caccagggag
     6841 gtaccgacga gcctccagtg ccacgccagg atccaggcgg tgttcgcggc cgcgttcagg
     6901 atgaacaggg ccccgtcccg gccggtccac gcccttcgga caccctccgc ggaccggaag
     6961 gtttcccggg cggctgccgc gacatagccc gccagcagga ggtagatcag gccccagatc
     7021 gagaaggtga ttcccgcggg cacgaagagg ttgggcagct cgtcggagag ctgtcccgta
     7081 ctcacgccgt tcaggggaag ggaggaggcc aggccgttga cggccaccat cccggtgaag
     7141 gccaggatgg caaggacggc ggccgcccgg gccgcccgag ttccgcttgg ttcggagttc
     7201 atgtcttcca tggtaacgga aaggcccggc cggcgccagt ccggactccc ttgccgtaga
     7261 ggcccccagc ggctaggatg ggaacactta ggaggctcca tgaacctcga cgcatcggag
     7321 acccgggacg cggcatcccg cgcccagggg ctcctcgccc gtgcccggga aggcatggcc
     7381 cggcgcctgg tgggcaagtc ggaactggtg gacgggatcc tgaccgctgt ggtcgcggac
     7441 ggccacgtcc ttttggaggg cgtccccggc ctggccaaga ccctggcggt caagaccttc
     7501 gcggagctgg cgggcctgac cttccggcgg gtgcagttca ccccggacct cctgcccggg
     7561 gacatcacgg gcagcctctt ctacgacgcc cccacgggac gcttcgtgcc ccgggagggg
     7621 ccggtcttca cgaacgtcct cctggcggac gaagtgaacc gcgcccccgc caaggtccag
     7681 tccgccctcc tggaagccat ggaggagagg caggtgacca tcggagaccg cagcatcccc
     7741 ctgcccaagc ccttcttcgt cctggccacc cagaatccca tcgagcagga gggtaccttc
     7801 cgccttcccg aggcccaggt cgaccgcttc ctcctcaagg tcctggcgga ctatccggat
     7861 atggaggagg aactcgagat cgtccgccgg gtcgcggacg gaatgtccgc agcccacaag
     7921 cccctcctgt ccgtcgagga catcgagttc ctccggtccg ccgcccggtc cgtgcgggtg
     7981 gagccggaca tcctggacta cgccgtccgg ctggtccgcg cctcccgccc cacgggggac
     8041 gacggggcac cccaggccct ccgagaactc cgtcgctaca tggaatacgg ggcctcccct
     8101 cgagcgtcca tctacctcta ccgctgcgcc cgggggggcg ccctcttcgc gggccgggat
     8161 ttcgccctgc ccgaggacgt caaggcggcg gccctgccgg tcctccgtca ccggatcatc
     8221 ccctcctacg aggccgaggc ggaggggatc acctcggacg tcgtaatccg ccgactcctg
     8281 tcgatcgtcc cggtacccta gatgcccgag gaagcggccc ggcggaccct gcgcaggctc
     8341 ctcttcacgg ccccggtgct ttcccgggcc ctcctttcag gcggtttccg gtccgtgttc
     8401 cggggccggg ggatcgagtt cgacgccctc cgggactacg acgccgagga cgacgcccgg
     8461 cagatcgacg gacgggccac ggcccggttc gggcgggcct tcgtgcggac ctaccgggag
     8521 gaccgcaacc tgacggtgct cctggtactg gacgagagcc agtccatgtc ctacggcacg
     8581 gatcggaccc ggcgagaggc cgcggggatc gcggcgtccc tgctggccta tgcggcggcc
     8641 ttgaacggca ccttcgtagg cgccctcttt ttcgacaccc gcggggtggg tttcgtcccc
     8701 ccccgggcgg gccggcgtcc cgccctgtcc ctgaccgagc gcctggccgt cggggaagcc
     8761 ggggcccgcg gacccgcggc ggtccggccg actgcgggac gaagcgccgg gctggcgggc
     8821 gccctggaag cggcacgggc tgtcctcaag cggcggtccc tggtcttcgt actctccgat
     8881 ttccgggctg ccgcggacgg cccggccggg tactcggggc ccctgggcag gctggccgcc
     8941 cggcacgacg tggtggccgt ccgggtcacg gatcccgcgg atacggcccc ccccggttcc
     9001 ggcgcggcct cggagatctt cgacgccgag agcggccggc ccgtcttcct ggtaccgggc
     9061 tcgaaaggcc tgcgggaagc atggtccgcc ttccagggcg gaatgcgtct ggactgcctc
     9121 cgggccctgc gggcttcggg cgccgccttc ctggagctgg ggaccgggga cgacccgggg
     9181 aaggccctga cggccttctt cgaacggcgc aggaggggcc gatgaggctc tcggcggcgg
     9241 tcctgctttg cctttccctg acggcggcgg gcgcccaatc ccgggtccag gtctccggga
     9301 cgaccgtgac ccccgcccgc ccctacgtgg gggatctggt cgaatgctcc gtgaccttcg
     9361 atcccgggac cgcccggata cccgagggtg tcctggatct tcccccccgg gccggaacgg
     9421 cggaggagac ggagcttagg tccgcggagg tgcgcaggaa ggcgggctcc tgggtctaca
     9481 cggcgcgctt cgtggcctgg gttcccgggc ctgcccgggt cccggtgccg gcatccgcga
     9541 atttcctcct gcccgagatc cgggtggaga tcgcctcggc cctggacgac ttcggccgtt
     9601 cgcccccggc ctacaaggac cccttggagc ttccgggcac ccggctcctg gtctggggcg
     9661 cgtccggggg cctgctggcc ggggcgatcc tggcctggtc cgtcgcgttc ggtctgttgc
     9721 cctggatccg gcggctccgg cgggcctgga aggatggacg ggcgggccgg gatttcggcc
     9781 gctccctgga ctacctggag tccgcggccg ccgagttccg tcccgaggag ctctgggctc
     9841 tcctgtccaa gtccctccgg gaatacctgg ccgcccggtc ccggctgccc taccaggcgt
     9901 tcacggcccg ggaggcccgg gcggcatccc cggaagccct gccccccgaa gcggccgagg
     9961 aggccgcgga cctgctggcg gagggggaag ccgtgcgctt cgcccggtcg gaccgcggcg
    10021 cgggcatccc ccgggccctg gagcgttccc gggaaatcct tcgctccgtg gaggaggcgg
    10081 cccgtgacct ccttcgataa tcccctggcc ttcctcctgg tcttggccct gcccggatgg
    10141 ctgttcctgc gggcccgggc cctgcgccgg ggcggaagcc tccggtttcc cctggacgag
    10201 gccgcgggaa ggcgcttcga gggtccctcg gggttccgga aggccgcgtc ccggggagcc
    10261 cttgtcctcg gcatcctggc ctggctgggc ctggtggtgg ccgcagcggg cccccaatcc
    10321 gtggagcgca ggacggtctt tccgaaccgg ggggacgacg tgatgttcgc cctggacctg
    10381 tccccctcca tgggcgcccg ggacgtggaa cccgaccggc tctccgcggc caagacgctc
    10441 ctggagctct tcctccggga agaccgggcc gattcggtgg ggatcgtggg cttcggtcgg
    10501 gaggcagccc tgctctgtcc cccgaccacg gattacgccg ccctccggga acggctggcc
    10561 cgggccaagc ccggggcgct gggagacgga acggccctgg ggctgggact ggcccaggcg
    10621 atccggcacg cggctcgggc ccgggggacc acggaccggg tcatcctggt gaccgacggg
    10681 gagaacaacg ccggttccat ctcccccgag gacgcctgcg acctggcggc ccgcctgggg
    10741 gtgcgcttga ctatcatcgg ggtcggcagc cgcggcgacg tgcccctggc ctacgaggat
    10801 ccccggacgg gggagcggct ggaggggacc tacgcgagcg gattcgacga gtcggccctg
    10861 cggaccctgg cggcacgggc gggaggggaa tacctgtccg cccgggacgg acccgccctg
    10921 gagcgcgcct tccgggcggt ttcggggcgc atgcccggcg cggagggggc tcgctccgtg
    10981 accgtccgaa ggtcccgggc ggcctcggtc ctggcggttt ccgtagtcgc cctggccttg
    11041 tcctggctgc tcgggctcgc cgcccggggg tggctgtcgt gactttccta cggccggagg
    11101 ccctgtgggg cctcgtcctc ctggctccct acgccgtact ggaggccctc tcggtcctgc
    11161 ggggaggcgc gctggcgggg cgcttcgctc cgggcgcgga agcgggtccg tcccgacggg
    11221 cctggatacg caggcgcgcc ggaaaggccg tcgcgggggc ctgggcctgg gcgttcgccg
    11281 tcttcgccct ggcgggccct tcctggggat tccggccgac ggccggcgcg ccgtcggggg
    11341 tcgaggcggc cctagtgctg gacgtctcca actcgatgct ctccccggac ctccctccct
    11401 cccggctgga ggccgcccgg ggcctggccc gggccctggt acgggcccgg ccggaggttc
    11461 agtattccgt gaccgccttc aagggggggg cggtcctcct ctgtccccgg acggacagcg
    11521 cggacgccct ggaccaggcc ctggaatggg ccggtccctc ggtgctcacg acccggggca
    11581 cctccgcggg ggaaggactc cggacggccc tggagggttt ttcccccgac cccggccgga
    11641 tccggtggat cgtcctgttc agcgacggca acgaccttgg cgccgccgcc gccccggccc
    11701 tccgggagat ccgggagcgg ggggcccggc tgttcgccgt gggctgcgga ggggtggatc
    11761 cgtcccccgt ctcggacgaa accggggctc ccgtcctgct tccggacgga agccccgccc
    11821 ggacggccct gagggcggat accctgcggg cctgggtccg ggagggagac ggactgtacg
    11881 tggacctggc ggaccccggg gccctgagga tcctttcgga cgccctggat tcccgtcccg
    11941 gatccccggg agcccgtcct gcccggcggc ccgcggaccg gaccgggacg gccgccttcc
    12001 tggccctgct gggagcggcc gcccgggccc tcctgggcct tcctttcctt ccgggccggc
    12061 gccgcggacc ccggacggcc gccgccctcc tcgcgctgac gatcctgacc ggatgttccg
    12121 gcccccggct ccgggtcctg gagggaaacc gccgcctgcg gaggggacag tacgaggatg
    12181 cgatcgccgc ctacctggcc gcgggcgaga aggccggcga ggggatcgtg gccctggatc
    12241 tggccacggc gtacagccgg atgggggagg gcgccgcggc ggatcccctc ttcgccctag
    12301 cccagtcctc ccggctttcg gaggtcgccg cggcggccta ccacaaccag ggagtccggc
    12361 tgttcgaggc ctcccggttc gacgaggccg cgcaggcgtt catgaacgcc ctgcgactgg
    12421 tcccccagga tctcgagacc aagcgtgccc tggaactggc ccgcgccgcg gccgcgtcgt
    12481 cccgcctgtc cagggcgtcc cggcggcagg cctcggcgat cggggaggga ggccgggacg
    12541 aggccctgct atccctgctc ggccgggccg agtccggctg gttccgtccg cccgcctccc
    12601 cgtccgcgga ttccggaggg ctggaccatt gagcggtccg gcccgccgac ggatcctgtc
    12661 ggccgtcctc ctcggcgtag cccggatcct ggccgcccag gaggtgacgt attccgtgac
    12721 gatagccccg gagccgccga ccgcgggaga ggagttccgg gtgtcggtcc tgcttcccgg
    12781 agcctccgcc cgggaggtcc gggcggccga gcccgcggcc tccggggacc tggccttcgt
    12841 ttccctggcc gtatcgtccg cggtgatcga ctcccccgag ggttcccggc cgggggcgag
    12901 ggtggtctat gccttccggc ccctgcgctc cggagatctc cggatcccgc ggctcgaggt
    12961 ctccgtcgcg ggctccccgc ttccgctcgg gcccctggcc ctggaggtcc ggccctccgg
    13021 agccgccccg cccccggcgc tctacgagtg gaaggccccc gcggcggtca tccggtggca
    13081 aagtttttcc gcccgcctgg agccgagggc gccttcggct ccggccctcg gggactacgg
    13141 gttgcccgcc acgaaggggc tgtctttgga gcccgccggc gggtcggcct tcgtggggat
    13201 cgccctggag gagggggaac tcttcctgcc tccggcggca tcccgggagg gcggccgcgt
    13261 ttccgagggc gcccgggtcc ggtccctgcc ggcgcctcgg gagctcgcgg ccagccgggc
    13321 cgtgggggac ttcaccctgc gcctgatccg ggtcggggca ggtccggtcc gggcgggaca
    13381 gaccctgacc gtccgggcgg aggttcgggg caggggcaac cttccgatcc tggatccccc
    13441 ggagatccgg atccgggcgc ccggcggcgg accgatatcc cctccggaga ccgtaccgat
    13501 ctcggagatc cggatcgccg ccggggccta cgaggggatg gccgcccggg agctccggtt
    13561 cacgcccgag gttccggggg actatgtcgt ggaatccggg cccttcccgt tcctgaatcc
    13621 cgatacgggc gaactgcgga ccctccgggc ttctcccctg aggatctccg tggatccgcc
    13681 tccccgggaa gcccggccgc gaacggagct tcgcaccctc ctggacgccc ggatcgccgc
    13741 cttcgccgat tcgggctccc cttggtcggc ggccgcgtcc cgggcgcggg acggggacgt
    13801 agaggcggcc ttgcacctcc taccggtgac gtccgccccg gaagcctcgc atctcaaggg
    13861 aatcctcctc atggcggagg aggacggagc cgccgccctg gccgccctgg gcgcggcgga
    13921 acggcggagc cgatggctgc ccggcctggc ggaaaccttg gaactctgcg agacgacttt
    13981 cggggtgggt ccccggatcc gggaccggtt gccccagccg cgtatcttcg gcgccctctc
    14041 ggttgttttt gcagcggcgg gactctcgat cctgggactg ttccggctgc gggcccggtc
    14101 cgccggaggg actccgggag cgataggccg agccctggtt agcgcggcct tcctctggct
    14161 cctcctctgc ggggcttccg ccctggaacg tcgcgtctcc tacgcggtcg cggcgtcccc
    14221 ggattccttc accgtcccct cggaatcggg gaccccgggc gggtccttcc agggacgggc
    14281 cgggatcgtc ggggcggttt cctcggattg gacgctcctg cggttcccgg acggtcggtc
    14341 cgcgtggttc cgtaccggcg acatacaccg ttactgagga aggacatggc ggatttcgga
    14401 aagatcctcg acgaatggga ccggcgatcc cggcgcgatt ttcgcagacc ccgccggccg
    14461 gacccggagg aggtcgagga agggaaaagc cgccgggatc ccgcccggga ggcccttgag
    14521 agggcgatgc tcgcgggcgt ggatccgtcc cggaaggggg aggaggggcg ggatccctca
    14581 ccccttagcc gggccgagat cgaggccctc ccggtggagg ccgtcctgga cctccacggc
    14641 cttacctcgg aggcggcctg ggaagccctg tcccggttct tccgggacgc ctcctcccgg
    14701 ggcttgacca aggtcctggt gatccacggg aagggaaacc actccgaggg ggagccggtc
    14761 ctgaaaagga cggcgctgcg tttcctggaa acctcgtccc tggcgggacg gcacggcacg
    14821 gccgagcgga ggctgggagg aagcggggct gtctgggttc tggtgcgaaa gggggctcag
    14881 cgttcccggt agatgatccg tccgcggttg aggtcgtagg gcgaaagggc gaccttcacg
    14941 gtgtctccgg ggacgatccg aatgtagtgc ttcctcatct tcccggacag gtgagccagt
    15001 atcacgtgcc cggtaggcag ttcgacccgg aacatcgtat tcgggaggga ctccttgacc
    15061 ttgccctcca cctctatcgc ttcttccttc gccacggttc ctccaggggt ctgattcgcc
    15121 cggacccggc ccgggaagga cgcgagtctt tcccgccttt ctcggcttgc aggggaggtc
    15181 cggacggcta taatagtatg ggttccccgg ggggcatgtc aacgggttga cattcccgcg
    15241 gttttccggc atggtgccga tgcttttttt tatcatagcc caggagggga ccatggaagt
    15301 ggacttcgtc gaggaaatga aggaatcact cctccggcag aagcgggaga tcctggacaa
    15361 cctgatcgcc acgaacgcgg atttccgcgc catcgtggag gagatggatc cgaaggattt
    15421 cgcggacgtc gcgtccgacg acatcgaccg gaagatgatc gaagccctcg gttcccagga
    15481 catcaagcga cttcgcgcga tcgactcggc cctggcccgc atccagctcg gaaagtacgg
    15541 gctctgcgtg aaatgttcca agaagatacc ccgggagcgg ctggaggcca tcccgtatgc
    15601 cgtgctctgc atagagtgcc agaagagcga ggaacgccga aaccgctgac ggcggccggg
    15661 cgtgcatcgc gcggggctgt tcaaatagct cggttagctt tttggacagc tggtttcagg
    15721 gttgcagttt caaaagggtg ttgaaaaagg cctatagcct ttttcaacac ccttttgaaa
    15781 ctgcaagggg tgtccaataa gtaaccgacg tattggacac cccggttctc attccagggg
    15841 acccgtcgtc catccgttcc ggccgagcac ggccagctcc cggtaccccg cctcccgggc
    15901 caagtcaagc ccgatccgtc tccaatgcgg atccaggtgg gcggccgtgt gggcgtccgc
    15961 cccgatgacg atcgggatcc cgcgccgcct cagttcccgg aggatgggca ggctcgggta
    16021 gggctcggag gtcttgcccc gggccggacc cccggtgttc acctccacgg cgatgcgggt
    16081 tcccgtcagc gcctccgcga cctcgaacgc ggcggaaagg tactcccggg agccttcgtc
    16141 gaaccagcgg ccgtccttgt tgtgcatctt cacgatgtcc agatgcccca ggatctggaa
    16201 gcccccggtc cggacgagtc cgcccagttc ccggtagtag tcccggtata gcagggtccc
    16261 gtccccgccg tatcccgccc gcactccctc ccggaaggac tccgccgggc cgtccacgga
    16321 aaagaaggag tcccccgaca gacgggcgaa gtgaagggat cctatccggt agtcgatggg
    16381 cacggcggcg tatccgtcct cgggggggat gccgagtccc ggaacccact cgatctccag
    16441 gccggtcagg atctccatgc ggcccttgta ccgggccgcc gccgcccgga ccgcggccgc
    16501 gtagggcgcc acccggtcgg ggctcaggtt gcccggggac gggacgggga cgggggcgtg
    16561 ggccgagaat ccgagcacgc ggtatcccgc ggcaaaggcg gcgtccgcca tcgcgtccgg
    16621 ggtatcccgg ccgtcgcagt acgtggtgtg ggcgtggtag gtggcgagca tggggggagt
    16681 ataggcgtga ataggtgaat gggtgaatag gtgaataggt gaataagaag gtttcctatc
    16741 cacgattcac gtattcactt atttacttcc attcaccgac cccaccgcgg ccttcagctc
    16801 cgccagggcc tctcgcagga ccggcagtcc ggccctcacg tccccgcccc ggccgtcccg
    16861 gcctgcatcc tccacggcct tggccgcgtt ccccaggcgc cgggcgctga ggttccaagc
    16921 cgcccccttg atggagtggg cgagctcccg ggctttctgc cagtcctccg cggattccgc
    16981 ctcggcgagt tcttggagtt ggctctccgc cttgcggagg aaacgaccga ggagatccag
    17041 cacggtcggc cggttaccga ggaaggtcgc gaccgcctcc tccagatcca ggatttccgc
    17101 gtccgtccct ccggcaccgg ctccgggcgc cggggtcccc tcgtcgtcgg gcgcggagcc
    17161 gtcgggctgg gggggcagga tccgggccag gagggcctcc aggtccgcct tcttgaaggg
    17221 tttggtgagg atgtcgtcca tgcccgaggc caggcacttg tcccgctcgc ccttcagggc
    17281 gctggctgtc acggccacga tggggccgcg gtacccttgg gcccgcaggg tctgggcggc
    17341 ctcgtaaccg ttcatccggg gcatgaagat atccatgaga accacgtcga aggatgttcc
    17401 ggttccccgc tccacggcct ccacgccgtc ggaggccacg gtcacccggc agcccagccg
    17461 ctccaacagc agggtgaaca gctcctggtt caccgcgtga tcctccgcca aaagcacgga
    17521 ccggccttcc ccgaccctcc gttcctcccg ggccggagct ccgtcctcca cggcctccag
    17581 ttccgggacc tcggtgaggg ccttgtccag gacttcgtgg agttcctcgg ggttgacggg
    17641 cttggacacg tcggcctcga accactgcag gagcttcatc ttggcttccg ccccgcgccc
    17701 gccctcgggg atcatgagca ggagccggac cgagtccagc tcggggtccg acttgatctc
    17761 gctggccagc cgccagccgt ccatccccgg catctcctgg tcgaccaggc agaggtcgaa
    17821 gggaatcccc gtccggcccg cctcccggag gatcgccagg gcctcctccc cggaggcggc
    17881 cgcccgggtc tccataccga aggaggccag gacacggagc aggaagtccc gggctcgggg
    17941 atggtcgtcc acgcagagca cccggggggt cgggacgaag ggcgggtagg cgagggggcg
    18001 gtcgtccgcg ccgggctcga gggggacctc gaaccagaac acagatccgc cgtccttgcc
    18061 cggcaggtag ccgatgcggc cccccatgag ctccgagaaa tgccggctga tcgccaggcc
    18121 gagcccggtg cccccccggg tcagggagga gaccgcctgg gtgaagggcg tgaacagccg
    18181 ggggcgcacg gattccggga cccccgtgcc ggaatcggcc acgtcgcagc gcaggaaggc
    18241 ccgcctctcg ccgtcccttc gggaaaggga aaccacgatc tccccggacg gggtgtgctt
    18301 gatggcgttc ttgaggaggt tgacgatgat ctggcggaac cggcccggat ccccccggag
    18361 ggtccgggga agccgctcct ccgtatccag gatcacctcc agccccttct tgtgggcgtc
    18421 caggatcagg aggtcccccg cctgggagac cacggaccgg aggtcgaatt ccgtcctctc
    18481 gagctccatc ttgccggcct cgatcttgga gaagtccagg atgtcgttga tcaggcccag
    18541 gagtatgtcc gcgctgaacc ggaccgtgcg gacgtagtcc atctgctcct tgtccagcct
    18601 cgtatccccc aggagctccg tcatccccag gatggtctgg atgggggttc ggatctcgtg
    18661 gctcatgttg gccaggaata tgctcttggt ctgggtggcc ttctcggcct cctccttggc
    18721 cgccaggagc tgcagctccc gacgctttcg ctccgtgtcg tcctcgaagg cggccaggat
    18781 atagactccc ccggaaccgc cgggagcgtc cacccgggac aggcggatcc ggaaccagag
    18841 cttgccccgg tccgcggacg acaccgggct ttcgagctgg gccgaagcct gccggccggc
    18901 cacgagttcc cgtacgtagt gggagacctt gaagcttccc cggagttcca gccagtcgaa
    18961 gagggatccc tccagggctt ccggcgcctg cccggccagg gagcggaatg tgtcctcgaa
    19021 ggcccggttc cagaagacca gtcgaccctc tccgtccgtc agcgctgcgg atacggggta
    19081 ttgctcgaat gtctcccgga acggggcacc cggacggggt tcgcgcatcg gcgtctccct
//
LOCUS       DAOTEI010000055        18840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1944_length_18840_cov_4.847219, whole genome shotgun sequence.
ACCESSION   DAOTEI010000055 DAOTEI010000000
VERSION     DAOTEI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1944_length_18840_cov_4.847219"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..695
                     /locus_tag="P5313_07235"
     CDS             198..695
                     /locus_tag="P5313_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY80191.1"
                     /translation="MEEALYVKETAQTIYIRAQGHVTAALCSDLKGRIFERLEAKPPV
                     ENVYVDLSSCDYMDSTFLGLLVGINKRFLRFSERPLTVVRPTPPCTELLRTIGILRLV
                     KVQEESVPFPDSMEAIVTAEKATAEFLLDVHENLMDLSDENRSRFSTLYSVLKSQINQ
                     PEEEP"
     gene            complement(769..1311)
                     /locus_tag="P5313_07240"
     CDS             complement(769..1311)
                     /locus_tag="P5313_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRY80192.1"
                     /translation="MSFKRTVLVSILALSFLAGVSAQQITRVAVVDLAKVYLTYSRDS
                     AAVRKLEEDKLKVQAEIDRMSNEIKQLQVRRAEAIARSDQAEVLRMDQELYRRAQYLS
                     EYYKAKQAELEDRSRRLMESSDFSQLVYRTIQGIAEADGYSVILSSHSTGSVTSAIIW
                     YSPAVDVTDKVIQALIGKAQ"
     gene            complement(1325..3856)
                     /gene="bamA"
                     /locus_tag="P5313_07245"
     CDS             complement(1325..3856)
                     /gene="bamA"
                     /locus_tag="P5313_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRY80193.1"
                     /translation="MKRSIGAVLFLAILASGIAAQPAADWYLGKTIKEIEFSGIKRVD
                     RNELDSVVQAYKGKAFSESLWMDLQASVYELDYFDEISPIALPGDAEYSTVIIKFTVT
                     EKPWIETVRVDGARGLRPGEILGAALLKSGDIYNQAKARADERAVRKLYIEKGYADAT
                     VTSFTEPGRGENSLILVFQVSEGSQLAIQELRFEGNAALAASSLKSKLSLKEQGLFQP
                     GLFQEQKLEADRIQIENLYQSRGYVDARVVDILRELSRDEGAQKTRLILTFVISEGEQ
                     YSFGGLQFSGNRIFPTEKLSALVRSKEGMILNYPTLLADKQRIEDLYYENGYIFNRIE
                     MNERRDPDRRVIGYTLEIVERDRAHIENVIVKGNKKTKDHVIYRELPFEVGDIFSKTK
                     VITGLRNLYNLQYFSALTPEMIPGSAENLMDIIITVEEQSSADIQFGLTLSGIGASTP
                     EFPISGLIKWNDRNFLGNGQSISVGITASPDTQEVTLGFSDPWILGKRMTGSVELSFV
                     HKGMQALQDTLGPVFAYGSPGAVPDPYTSWAEYEAAGYVVPDEYYMDYEYWNINLTFS
                     LGYRFVFPVGTLGLSGGVRLGLTNQSYDQGLFRPFSEDLASSAGQWINANSLIFRTYM
                     NALDLWYDPSKGYFASQKFTWAGLFDFEQDQFLRTDTKLEGYVTLFDIPIGEDWSLKW
                     VLGGHTGLSLLLPKPGSSLSVTDNSKLYIDGFFVGRGWRDLTEETFGGTILWENWVEI
                     RMPLVEQFLSFDMFLDAATVRTDQGLLSFKTGASGGLDSTKTSLFDLGFENLAFSLGA
                     GFRFTIPQFPFRFYFAKRFFMNGAGKFEWATEGMEFVISITQTTL"
     gene            complement(3869..8236)
                     /locus_tag="P5313_07250"
     CDS             complement(3869..8236)
                     /locus_tag="P5313_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRY80194.1"
                     /translation="MKNRGKSRLPRGAGAVVAVLLGAALAALPLDRAGSAWARSVLAR
                     AAFAAEDASGLSISYDRASPSIFGALRLSYLGLTDAGGRNLLRADRLEAEYDLAAAAA
                     ALVGKAAAGRSLDRITLRNVEIDLDLSKDADLIAKLRSTLGGGSGSGDPLGRLAISAR
                     RLRLSLKDPERKASWTAEIRSLDLLLTERELVVALDGTFSGSFRDPRFPFERVTVSIS
                     VRGSSSRDLTAARYSIALSARSDAGILERQEFSVLLRDGVLEARKVRDDAPFDLLARY
                     DTRAGTLDADLRFESLYLSRLGHPTGSREKAWAPWFRRPYSGNIRLRYPEAWKNPRIS
                     AELSGFLPPGFLGDDFEFRLDASGGLDGIDLKTLEARSPNGTARVRGTLRPDLSSLAL
                     TADLDYRAREGSFPLKTSLRAVGEGRELFLFSDTAELGGVEFAKLAVFAERAPGTLDF
                     RGSLDLPSRESDGSLGISGAVEDAGPRRISWEGSLSLGARPFVETALVLDPYDLAPLE
                     PLLSALLEPKAADFLSGLSLGGGLTLSSDFSRFSYSAADFRIAPAGAAGEDTYAVLNL
                     AGTSAEISISRLSASLGGFPIEGRVDAEFDLPRAGFRADLTLRDIPFAFQGDYDGGRV
                     FLTGDYGFSMVATVSGPETRGVVAFKALPLPLREGTILATGDAVGEFRSPRDWGLRIS
                     SLALEPEGALKGRLPIVRLAGAVGPEGGVFPDIAVEDGRSRLSGSARYAGGAELSGIR
                     EVELNLAGEGGEALRAVGNFRDRTVAGRVDLQSFSLSRIFGSALTGRLDGGITASGTL
                     EDPKVDFSLALREGSLRGRSLAAAAEGGLDREGLQIRDGSGRYGIHQVESTRIDLGFR
                     TGSLSASGAYTGFLAGDVARFRFETAGDLEAAAYGASGFPNLSDIAKSLTLRGTILDF
                     SMAKNYSARWPFTLERTGRDLVFQGGSGSEVQVRVLDDGSFLARAMPPFPLAAVVVGK
                     VADRQIAMDLSDIRVEMTSLWPILPLPEVRFLSGIATGSLSVRGKASDPEIYGSLRFQ
                     DALLEVPNYVNAPIGPVTAPLEANGRQVTLVQPVVGIGQTAKAAVSMSFEMSQWIPRS
                     FSLRARSVESTQVPLKTRFLGMNIAGTADADILLAAADGKLELTGALTIPRAEIVIDP
                     SIMQAGAEGDGRVPNVDTKIDLAVRIGKGVMVYFPSKEFPVISGQADPASRLSVSYDD
                     KQQSYALKGNAVLRGGNLFYIQRNFFLKTGRMVFNENQYSFDPRVTLEAELRTRRGQE
                     TVKIVLKAENASLFNFQPTLESTPSLSQTEIAALLGSDLLASDDGSDVDIRRTLIAST
                     DILPQLNFINVFERNTRRLLGLDLFYLRTELVQRWLLDVARLDADVEGGVTLADYLDN
                     TSVFAGKYLRDDVFLRGSLRLQQDQPLVNRSSLRLDSEIGFELTTPFFLFDWSLSFLH
                     PEDLFISDNSFRFTWKLTY"
     gene            8317..8958
                     /gene="tmk"
                     /locus_tag="P5313_07255"
     CDS             8317..8958
                     /gene="tmk"
                     /locus_tag="P5313_07255"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRY80195.1"
                     /translation="MKYQATASREVWDRFIVFEGIDGTGTTTQRNLLSETLRTRSIPF
                     HSTAEPTDSEIGRLIRKILAGDVRVRPETLAYLYATDRNEHIFGEGGIREHLDAGEVV
                     VCDRYFFSSLAYQGSACGPELPERLNQGFPLPSLLVYFELSPDRALSRISGRGVPDIF
                     ERGDFLSQVSRAYEDILNRCADQGLRVIRIDGSRTIPEVADDIQRAVLSAIEG"
     gene            9039..9635
                     /locus_tag="P5313_07260"
     CDS             9039..9635
                     /locus_tag="P5313_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80196.1"
                     /translation="MKRSVLFAILLIAAAAGLTAYPVYFKEQYYRLFHTHYIQYPDDA
                     IENIYWLERARDADFCNPLYALAKIENERQWERYRYLMNMHLELKLIEQHLALGSKFD
                     KRVAYFYNAPWKDQNLESLKIAETAYKAALAYWPAAVEWARKAEGLRFVRLPEVQAWE
                     DQAQRIVSGDLDYSDIIADELARVSRVRKAFESMQFTY"
     gene            complement(9784..11229)
                     /locus_tag="P5313_07265"
     CDS             complement(9784..11229)
                     /locus_tag="P5313_07265"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRY80197.1"
                     /translation="MMSAYGEFPDSGYYAACNSFPRNTAVEVVNLENGKSVTVIVTKG
                     LENPGIFLLLSPEAAKALSMEPGSLSRVRVSAPRNIAETPPAGSGPGVDPDFNPSLLA
                     SRAGVSTPTEEPRSTIPVEVPAVAAEPGAAGTASEEPAIPEPVPSNLAPPPSAVVNAE
                     VSPKAPTEPPEVSAPSEAPEPSLPPAPVPESPSAKEVPVVRPELIARPGVVPLESGPV
                     TAVLPAPETAAVREVPEDKAVVHGLNRPAPAALPVEAALADPVLVPDELPETALTRKA
                     WPADPAPSVALADLEIRMDDSGKPEALSRSQTGSPDSDRAPEVGLAEPIAVAKTEPEK
                     QPEPSPVVPPADSVPAEPADVVVTMEPAAPKPPEAPTKPAAAPAAPVAAAPVPVKPSS
                     PPKEPAAAPDSTSLIPGRHYIQVGAFKTTAAVETAAARLRGAFPVTIDTVTTGPGPLY
                     RLFVGPLGRDETGVALLQVRSLGFKEAFLKN"
     gene            complement(11330..11833)
                     /locus_tag="P5313_07270"
     CDS             complement(11330..11833)
                     /locus_tag="P5313_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80198.1"
                     /translation="MKRTVSACVLAVLCLAAPLSGQTFSVALLEDSGGPEAPDLSDAL
                     LGGCLDHLFAAGFIATNETIGRIGRADFQSSVLGLKSAREGYVDYVALVWIRYADTPS
                     DPPVRFPESLAWRLMRVRDGSALAEGSEKPERFDSGTVEERRERLSQLGKTLAAVWAR
                     TLRKERG"
     gene            11948..12505
                     /gene="loaP"
                     /locus_tag="P5313_07275"
     CDS             11948..12505
                     /gene="loaP"
                     /locus_tag="P5313_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HRY80199.1"
                     /translation="MQYYAVQVKSRCEDEYVRRLRSNPAVSGAEILLPKRTVILRKGG
                     IKRRGTAPVFPGYVFLGLDEAVNPELRWLVRRTPDFYRFLPSNQDPVPLGGRDLDLLT
                     HFISFGERADTSKVAFDENDRIVVLEGPLKGLEGLIVKVDRRKGRAKVRLDMCRNSFL
                     IDLAFSVVDRAGKGQTEPHGESRGT"
     gene            12483..13970
                     /locus_tag="P5313_07280"
     CDS             12483..13970
                     /locus_tag="P5313_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRY80200.1"
                     /translation="MENREEPRIYVIGAGFAGQSIAREIKARGILGRVVAFLDDDPEK
                     IGRRLENVPVLGPIRDVVNLLRRTPADEAIIAMPSASRDYLRELYGLLKAAGFSRIRI
                     LPGIAQIVEGDAHLIQAREIDPQDLLGRGPVVIGLAESLSYLKEKRVLVTGAGGSIGS
                     ELARQLLSARVQRLYLFGHGENSIYQIDKELRLLQAGGVGVRTDIVPVIGELKDRAYT
                     THILKRLKADVIFHTAAYKHVPMMEENPVAAVENNVFGTWNLAEAAREAGTQRFVLIS
                     TDKAVDPVCVYGASKLISERIVREAQKKVGAGRKFMVVRFGNVLGSRGSILPLFQRQI
                     ETGGPVTVTDPEASRYFMTIPEACSLVLKTGGVGEGGESYLLDMGEPVRIKDLAEQLI
                     RFYGFEPERDIPIRYIGLRPGERTVERLHSERETLIPTTFPRVNRLVPDSDTDLPVDR
                     LLEELRPVCAPLEGREGEYRNRRRLRQILRTYIPTVEAPAHEPEY"
     gene            13954..15123
                     /locus_tag="P5313_07285"
     CDS             13954..15123
                     /locus_tag="P5313_07285"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY80201.1"
                     /translation="MNPSTDFVPFALPSLGPEEEEAVLAVLRSGWLTTGSVALQFERE
                     FASFVGAEHALAVNSATSGLHLALEALGVGPGDTVVTSPYTFASTAAVARHLGAEVVF
                     CDVVPGGYNMDPRRLEEVLASTRNVKAVVPVHVGGLPCSMPEILDLARRYGCAVVEDA
                     AHSFPSRLPGGFAGTLGDIGVFSFYATKTITTGEGGMVVTSDSRLAKRMSVMRMHGID
                     KPVWDRYTSKTASWSYSVVEAGFKYNLPDLLAAIGREQLKKADRFLEERRRIAGIYGE
                     SFRGLPFAELPPEGPGHAWHLYSLRLRPGSLQASRDEFVDRLREAGIGTSVHFIPLHT
                     MPYWAERYGLTPDRFPEALSSFQRTVSLPIWQGMGEDRARRVARAVAETGAGLSG"
     gene            15131..17230
                     /locus_tag="P5313_07290"
     CDS             15131..17230
                     /locus_tag="P5313_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HRY80202.1"
                     /translation="MPRDRNESLSADIPLSDLRDEGMIRVVTVRAPVCRGRITDIEVP
                     RLPRGYRAIRAEDIPGENRLAILGFDIPILARDRVAYAGEPVLLLAGPDPAVLEDLRS
                     TVRVSVQEEEPVLEWESFSPEQVIQRRSAVLGNPEKAFEAAAEVLEARYRTDSQEHLY
                     PEPQGALAVFDYDKLVIRCATQWPFHVRDCVRSVLGVKPQEVIVRPTPLGIPMEGRLW
                     YPSVLACHAALAAVLCRKPARILLSRDEDFRFTPKKARSLVTLRAGLDPSGTLTVLEA
                     VVVLNTGAYAPLASELLEQACLQITGSLSIPNLRVQGYAVRTNTPPLGAFSGMGAAHS
                     LFAAESLASRIARDREEDPLEWRARHFLRKGSPLLTGERLRDRTTFEEIGSRLKASSD
                     FARKHGSNELIRKRRSASRSEEAPRGIGIAFGYQANGASYAALASGTFTVETTLNKDL
                     SLVISTSAIAGDEGIRSIWRSTAAGILGVPLGRVSISEVDTASVPDSGPSMLSRNISV
                     VNRLIERSCIAIRSRRFRSPLPIASRAVHRPRNPLRWEEDAVRGYPFESLSCCGAVVE
                     LELDTWSMEPRILGVWLCVDGGRIASERFARGTLSSSTLAALGQCFRERLPFEEGRVA
                     ENGYREYGLLSLAEAPPIEIEFLPWSDTDPLKGIGELPFLCIPAAFASAAAQASDLGG
                     ESLPFDGAEYLAGMEAV"
     gene            17227..17715
                     /locus_tag="P5313_07295"
     CDS             17227..17715
                     /locus_tag="P5313_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY80203.1"
                     /translation="MKLDFILNGEDVSCQAEAGDRLAYILRERFSLLGAKEDCRSGHC
                     GLCMVSLNGRAVPSCLVPAFRVRGAEVVTIEGFELTDDYADLRDGFRKAGAEPCEFCR
                     GAVFLSAAALLDERPRPEATEILEHLSAVFCRCHDPSALVRGVQTAAELRARRMYFRA
                     NR"
     gene            17702..18547
                     /locus_tag="P5313_07300"
     CDS             17702..18547
                     /locus_tag="P5313_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY80204.1"
                     /translation="MRIDEVIVPATLSEALSALKRNPDAVLCAGGTELFSEGPAGPAD
                     RNRTLISLHGIQELRHVSRTDLYVEVGSMTTLAELLALKEGFVPETLRSVVHGIGTFA
                     VRNLATLGGNLSVTDRFRDCFPILACMDALAEYRQGTASRWVNLNRLIGPRGTPEMPR
                     GEILTRIRIPLGGWDLGIAHKLGFPRINSRKSSLFVALARTDKRALSDIRILYACDRA
                     LRSREIESSLAGKKIPLLPRDIESAVGAYADYCSHAGIRESSAARFLTLVRRAFNQLN
                     EGVTA"
     gene            18544..>18840
                     /locus_tag="P5313_07305"
     CDS             18544..>18840
                     /locus_tag="P5313_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRY80205.1"
                     /translation="MKAPGRLFLLPAPIGDGDPLATLSPEVIEIYRSLRHFVVESERT
                     AGRLLSRILTPESLQETALNTLSEHTDPKAIPGLLEPALEGRDLGILSEAGCPCV"
BASE COUNT         3295 a         6215 c         6060 g         3270 t
ORIGIN      
        1 gttcgaacat ctcgtagaaa tccccgaggc ggaagaagag gatctcgtcc cggtgggcgc
       61 tcttgatccg acggtactgg tccatcaggg gcgtggtttc ggacatggcg gttgcattat
      121 aggcgggaac tcaggtagga tacaatgctg agggtgccgg tccccccggc ccgatccgcc
      181 tttcagcgag ggagcccgtg gaagaagccc tgtacgtgaa ggaaaccgcc cagacgatct
      241 atatccgcgc ccaggggcac gtgacggcgg cgctgtgttc cgacctcaag ggccgtatct
      301 tcgagcgcct ggaggccaag cccccggtcg agaacgtcta cgtggacctc tcctcctgcg
      361 actacatgga ttccaccttc ctgggtctcc tggtgggcat caataaacgg tttctccggt
      421 tctcggaacg ccccctgacc gtggtccgcc ccaccccgcc ctgcacggaa ctcctgcgca
      481 cgatcgggat cctgcggctg gtcaaggttc aggaggagtc cgtccccttc ccggattcca
      541 tggaggccat cgtgacggcg gagaaggcca ccgccgaatt cctgctggat gtccacgaga
      601 atctcatgga cctttccgac gaaaaccgga gcaggttttc cacactgtat tcggtcctta
      661 aaagccagat caaccagccc gaggaagaac cgtaacatca aggcgcgtgt gaaactcgct
      721 gaagctcgat agagcttcag cgccgccacg gcgcttcgcg ccgtggcgct attgggcttt
      781 tccgatcagg gcctgtatga ccttgtcggt cacgtccacc gccgggctgt accagatgat
      841 ggcgctcgtg acggacccgg tggagtggct gctcaggatc accgagtacc cgtccgcctc
      901 cgcgatcccc tggatggtcc tgtacacaag ctgggagaag tccgagctct ccatgagcct
      961 gcgggaccgg tcctccagct ccgcctgctt ggccttgtaa tactccgaga ggtactgagc
     1021 ccggcggtac agttcctggt ccatccgcag cacttcggcc tggtcggacc gggcgatcgc
     1081 ctcggccctg cgtacctgga gctgtttgat ctcgttggac atcctatcga tctccgcctg
     1141 caccttcagc ttgtcctctt ccagcttgcg gacggcggcg gaatcccggg aataggtgag
     1201 gtacaccttt gcgaggtcca ccaccgccac acgggtgatc tgctgggccg agacgccggc
     1261 caggaaggac agggccagga tcgaaaccag tacggttcgc ttgaaactca tgcgattctc
     1321 ctcgtcagag ggtggtctgg gttatgctga tgacgaactc catgccttcg gtcgcccatt
     1381 cgaatttgcc ggccccgttc atgaagaacc gcttggcgaa gtagaagcgg aaggggaact
     1441 gggggatcgt gaagcggaag cccgctccca ggctgaaggc cagattttcg aaaccaaggt
     1501 cgaacaggga ggtcttggtg gagtccagtc ccccggacgc cccggtctta aagctgagga
     1561 gcccctggtc cgtccgcacc gtggccgcgt ccaggaacat gtcgaaggac aggaactgct
     1621 cgaccagggg catccggatc tccacccagt tctcccagag gatggtcccc ccgaaggtct
     1681 cctcggtcag gtcccgccag ccccggccca cgaagaagcc gtcgatatac agcttggaat
     1741 tgtccgttac cgagagggag gagcccggct tgggcaggag gagggagagt cccgtgtgcc
     1801 cgcccaggac ccacttgagg gaccaatcct ccccgatggg tatgtcgaag agggtcacgt
     1861 agccttccag cttggtgtcg gtccggagga actggtcctg ctcgaagtcg aacaacccgg
     1921 cccaggtaaa cttctggctg gcgaagtacc ccttggaggg atcgtaccag aggtccaggg
     1981 cgttcatgta ggtccggaag atcaggctgt tcgcgttgat ccactgtccc gccgaggacg
     2041 ccaggtcctc gctgaaggga cggaagagac cctggtcgta ggactggttc gtcagcccca
     2101 gccgcactcc ccccgaaagc ccgagggtgc cgacggggaa gacgaagcgg tagcccaggg
     2161 agaaggtcag gttgatgttc cagtactcgt agtccatgta gtactcgtcc gggaccacgt
     2221 aacccgcggc ctcgtactcc gcccaggagg tgtagggatc cgggaccgcc ccggggcttc
     2281 cgtacgcgaa tacgggtccg agggtgtcct gcagggcctg catcccctta tggacgaagg
     2341 agagctcgac ggagccggtc atccgcttcc cgaggatcca gggatcggag aacccgaggg
     2401 tcacctcctg ggtgtccggg gaggccgtga tccccacgga gatgctctgg ccgttgccca
     2461 ggaagttccg gtcattccac ttgatcaacc cggagatcgg gaattccggg gtggaggccc
     2521 ctatgcccga aagggtgagc ccgaactgga tgtccgcgga gctctgctcc tccacggtga
     2581 tgatgatgtc catgaggttc tcggcgctgc cggggatcat ctccggagtc agggcggaga
     2641 agtactgcag gttgtagagg ttacgcaggc ccgtgatgac ctttgtcttg gagaatatgt
     2701 ccccgacttc gaagggcagc tcccgataga tgacgtgatc cttggtcttc ttgttgccct
     2761 tgacgatgac gttctcgata tgggcccggt cacgttccac gatctccagg gtgtacccga
     2821 tgacccggcg atcgggatct cgccgctcgt tcatctcgat ccggttgaag atgtagccgt
     2881 tctcgtagta gaggtcctcg atcctctgct tgtccgccag gagggtcgga taattcagga
     2941 tcatcccctc cttggaacgg accagggccg agagtttttc cgtggggaaa attcggtttc
     3001 cggagaactg gagccccccg aagctgtact gctccccctc cgagatgacg aaggtcagga
     3061 tcagccgtgt cttctgggct ccctcgtccc ggctaagctc ccggagaatg tccaccaccc
     3121 gtgcgtccac gtagccccgg ctctggtaga ggttctctat ctggatcctg tccgcctcca
     3181 gcttctgctc ctggaagagt ccgggctgga agagtccctg ttccttgagg ctcagcttgg
     3241 atttcaggga ggaggcggcc agggccgcgt tgccctcgaa ccgcagctcc tggatcgcca
     3301 gctggctgcc ctcggacacc tggaatacca ggatcagaga gttctccccc cggccgggct
     3361 ccgtgaagct cgtcaccgtg gcgtcggcat accctttttc gatatagagc ttccggacgg
     3421 cccgctcgtc cgcccgggcc ttggcctggt tgtagatgtc cccggacttg agcagggccg
     3481 cgcccaggat ctcaccgggg cggagtcccc gggcgccgtc cacccgtacg gtctcgatcc
     3541 agggcttctc ggtcaccgtg aatttgatga tcaccgtcga gtattcggcg tccccgggca
     3601 gggcgatggg gctgatctcg tcgaagtagt ccagttcgta gaccgaggcc tgcagatcca
     3661 tccagaggga ttccgagaag gccttcccct tgtacgcctg caccacggag tccagttcgt
     3721 tccggtccac ccgcttgatg ccggagaact caatctcctt gatggtcttg cccaggtacc
     3781 agtccgcggc cggctgggcg gcgatgccgg atgcgaggat agccaggaac agtacggcgc
     3841 cgatgctacg cttcatgatt cacccttctc agtaggtcag tttccatgtg aaacggaagg
     3901 aattatcgct gatgaaaaga tcctcgggat gcaggaacga caggctccaa tcgaacagga
     3961 agaacggagt cgtcaactcg aaaccgattt cggagtctag ccttaacgag ctccggttta
     4021 caagaggctg gtcctgctga agacggaggg agcccctcag gaacacgtcg tcccggagat
     4081 acttgcccgc gaagacggag gtgttgtcca ggtagtccgc cagggtgact ccgccctcca
     4141 cgtccgcgtc cagacgggct acgtccagga gccaacgctg gaccagctcg gtcctcaggt
     4201 agaacaggtc caggcccagg agccggcggg tgttccgctc gaacacgtta atgaagttca
     4261 actggggaag gatgtccgtg gaggcgatca gggtcctgcg gatgtccacg tcggatccgt
     4321 cgtccgaggc gagcaggtcg cttcccaaca gggccgctat ctccgtctgg ctcagggacg
     4381 gagtcgattc gagggtgggc tggaagttga agaggcttgc gttctccgcc ttcaaaacga
     4441 tcttgacggt ctcttgaccc ctccgggtcc ggagttccgc ctctagggtg acccggggat
     4501 cgaaggagta ctggttctcg ttgaacacca tcctgccggt cttgaggaag aagttcctct
     4561 ggatgtagaa gaggttgccg ccccgaagga ccgcgttccc ctttagcgcg taggactgct
     4621 gcttgtcgtc gtaggagacg gaaaggcggc tcgcgggatc cgcctggccg ctgatcacgg
     4681 ggaattcctt ggaggggaaa tagaccatga ccccctttcc gatgcggacg gccaggtcga
     4741 tcttcgtgtc cacgttgggg acccggccgt ccccctccgc ccccgcctgc atgatggaag
     4801 ggtcgatgac gatctcggcc cgtgggatcg tcagggcgcc ggtcagctct aacttcccgt
     4861 ccgcggcggc caggaggatg tccgcatccg cggttccggc gatgttcatg cccaggaacc
     4921 gggtcttcag gggaacctgg gtggattcca cggagcgggc gcgcagggaa aagctcctcg
     4981 ggatccactg ggacatctcg aaggacatgc tgaccgcagc cttggcggtc tgcccgatgc
     5041 cgaccaccgg ctgaaccagg gttacctgcc gtccgttcgc ctccaggggg gccgtaaccg
     5101 gaccgatcgg ggcgttgacg taattcggca cttccagcag ggcgtcctgg aaccggaggc
     5161 tcccgtagat ctcgggatcc gacgcctttc cccgtaccga gagagagccc gtcgctattc
     5221 cggacaggaa ccggacctcc ggaaggggca ggatcggcca gagggaggtc atctccacgc
     5281 gaatgtccga gaggtccatc gcgatctgcc gatccgcgac cttcccgacg accaccgccg
     5341 ccagggggaa gggaggcatc gcccgggcca ggaagcttcc gtcgtccagg acccgcacct
     5401 gtacctccga gccgctgcct ccctggaaca cgagatcccg cccggtccgt tccagggtga
     5461 acggccaccg tgccgaataa ttcttggcca tggagaaatc caggatcgtc ccccgcaggg
     5521 tcagggactt cgcgatgtcc gagaggttcg gaaatccgct cgcgccgtag gcggcggctt
     5581 ccaggtctcc cgccgtctcg aagcggaacc gggccacgtc tccggccagg aacccggtgt
     5641 aagcgccgga cgccgacagg gaaccggtcc tgaatcccag gtcgatccgg gtgctttcca
     5701 cttgatgaat cccgtagcgg cccgaaccgt cccggatttg cagtccttcc ctatccaggc
     5761 cgccttccgc ggcggccgcc aaggagcgac cgcgaaggga tccttcccga agagccaggg
     5821 aaaaatcgac cttcgggtcc tccagggtcc cggaagcggt gattcctccg tccagcctgc
     5881 ctgtcagtgc gcttccgaaa atccgggaaa gggaaaagga ttgaaggtcg acccgtccgg
     5941 cgaccgtgcg gtccctgaaa ttcccgacgg cccgtagcgc ctcccccccc tcccctgcga
     6001 gattcagctc gacctcccgg attccggaga gttccgcacc gcccgcatac cgggccgatc
     6061 cggagagccg ggatcggccg tcctccacgg cgatgtccgg gaacacaccc ccctccggcc
     6121 ccacggcccc ggccaatcga acgatcggga gccgtccctt gagtgcgccc tccggctcca
     6181 aggccaggga ggagatgcgg agcccccaat cccgggggga acggaactcc ccgacggcgt
     6241 ccccggtggc caggatggtg ccctcccgga gaggcagggg aagggccttg aaggcgacga
     6301 ctcctctcgt ctcgggaccc gatacggtcg cgaccatgga aaacccgtaa tctccggtaa
     6361 ggaaaacccg tcccccgtcg tagtcccctt ggaatgcgaa cgggatgtcc cggagggtga
     6421 ggtcggcgcg gaatcccgcc cggggtaggt cgaattcggc gtcgacccgt ccctcgatcg
     6481 gaaaaccgcc gagggaggcg gataggcggg agatggagat ctcggcggaa gtcccggcca
     6541 ggttcaggac cgcgtaggta tcctcacccg cggccccggc cggggcgata cggaaatccg
     6601 ctgcggagta tgaaaaccgg gaaaaatccg aactgagggt gagccccccc cccagggaaa
     6661 gaccggacag gaaatccgcg gccttgggct ccaggagggc cgacagcagg ggctccaggg
     6721 gcgcgaggtc gtagggatcc agcacgaggg ccgtttccac gaaaggtcgg gctcccagcg
     6781 aaagggaacc ttcccaggag atgcgccggg gcccggcatc ctccaccgca ccggagattc
     6841 cgagactccc gtcggattcc cgggaaggta aatccaggct gccccggaaa tcaagggttc
     6901 ccggcgcgcg ttccgcgaaa acggccagtt tggcgaactc cacccccccc aattcggccg
     6961 tgtcggagaa caggaacagc tcccggcctt ccccgacggc tcgcaagctc gtcttcaagg
     7021 ggaagctgcc ttcccgggcc cggtaatcga gatccgcagt cagcgcgagg gaggacagat
     7081 cgggccgcaa ggtaccccgg acccgggccg tcccgttcgg tgaccgggct tccaaagtct
     7141 tgaggtcgat accgtccaga cccccggacg cgtccagccg gaactcgaag tcatccccca
     7201 ggaaacctgg gggaaggaac ccggacagct cggcgctgat cctcggattt ttccaggcct
     7261 cgggatatcg caaccggatg ttaccggaat acggcctccg gaaccacggt gcccaggcct
     7321 tttcccggct tcccgtcggg tgtcccagcc tggagaggta cagggattca aaccgcagat
     7381 cggcatccag ggttccggcc cgggtgtcgt accgggcaag gagatcgaag ggggcgtcgt
     7441 cccggacctt ccgggcttcc aggacgccgt cccggagaag gaccgaaaac tcctgacgct
     7501 ccaggatgcc cgcgtccgat cttgcggaaa gggcgatgct gtagcgggcc gccgtcaggt
     7561 cccggctgga actcccccgg acgctgatcg agaccgtcac cctctcgaac gggaaccggg
     7621 gatcccggaa ggatccggag aacgtaccgt ccagggcgac cacgagttcc cgttccgtca
     7681 agaggagatc cagggaacgg atctccgcgg tccaggaagc tttccgctcg gggtccttca
     7741 aggacagccg cagccttcgg gcagagatcg ccagacgccc aagagggtcg ccggacccgg
     7801 atcccccgcc cagggtgctc cgcagcttgg cgatcaagtc ggcgtccttg gacaggtcca
     7861 gatcgatctc tacgttccga agggtgatcc gatccaggga ccgccccgcg gcggcttttc
     7921 cgaccaacgc ggccgctgcg gccgccaggt cgtattcggc ctccagccga tccgcgcgga
     7981 gcaggttcct ccccccggca tccgtcaggc cgaggtagga cagcctcaag gccccgaata
     8041 tggacgggga agcgcggtcg taggatatgg ataatcccga ggcgtcttcc gccgcgaacg
     8101 cggcgcgcgc caggacggac cgagcccaag cggatccggc gcggtccaag ggcaaggccg
     8161 cgagcgctgc gcccagcagg acggcgacga ccgcccccgc tccccgcgga aggcgggatt
     8221 tccctcgatt tttcaccctc gacaccttag cataaacata gcgcggcggt catgcgggcg
     8281 cttgatgagg tcccgtcgcc cgggtatcct ggagtcatga aataccaggc gacggcctcc
     8341 cgggaggtct gggaccgctt catcgtgttc gagggaatcg acggaaccgg gacgacaacc
     8401 cagagaaacc tcctgtccga gacgctcagg acccggtcca ttcccttcca ttctaccgcg
     8461 gagcccacgg attccgagat aggccggttg atccggaaga tcctggcggg cgacgtccgc
     8521 gtccggccgg aaaccctcgc ctacctgtac gccaccgacc gaaacgagca catcttcggc
     8581 gaagggggta tccgggagca cctggacgcc ggggaggtcg tcgtttgcga ccggtatttc
     8641 ttctcatccc tcgcgtacca gggcagcgcc tgcgggcctg agctcccgga acgactcaat
     8701 cagggattcc cccttccgtc cctcctggtc tatttcgagc tgtccccgga tcgcgccctc
     8761 tccaggattt ccggacgggg cgtcccggat atcttcgaac gcggcgattt cctctcccag
     8821 gtctcccggg catatgagga catcctcaac cggtgcgcag accagggctt gagggtgatc
     8881 cggatcgacg gatcccggac gataccggaa gtggcggacg acattcaacg ggccgtcctc
     8941 tccgcgatcg aaggataaac aaccccggcc caggaaggtt tccttccgcg ctcgagccga
     9001 taacggattc tggcgaatct tccgatacta tgatcggtat gaagcgctcc gttctgttcg
     9061 ccatcctcct tattgcggcc gcggccgggc tgaccgccta tcccgtatac ttcaaggaac
     9121 agtattacag gctcttccat acccactaca tccagtatcc ggacgacgct atcgagaaca
     9181 tctattggct ggaacgggca cgggacgcag atttctgcaa tcccctgtac gccttggcca
     9241 agatcgagaa cgaacgccag tgggagcggt accgctacct catgaacatg catctggagc
     9301 tcaagctgat cgagcagcac ctggccctgg gatccaagtt cgacaagcgg gttgcttatt
     9361 tttacaacgc cccctggaag gaccagaacc tggaaagcct caaaatcgcg gaaaccgcct
     9421 acaaggccgc cctggcctac tggcccgcgg ccgtggaatg ggcccggaag gccgagggcc
     9481 tccggttcgt ccgcttgccc gaagtccagg cctgggaaga ccaggcccag cgcatcgtca
     9541 gcggagacct ggactactcg gatatcatcg cggatgaact ggcccgggta tccagggtga
     9601 ggaaagcctt cgaatcgatg cagttcacct actgatcgta ctatccgggc agcatccatc
     9661 cgccggatcg caggatagtg aacccaccta cagagaaatc ccggtttccc cccggccctg
     9721 aggaaaaaga aacggggcat cccggaaatg ggacaccccg tcatcctaat ggccgggcta
     9781 ttttcagttc ttcaggaagg cttccttgaa tcccaaggac cggacctgca gcaaggccac
     9841 tccggtttcg tccctcccca ggggacctac gaacagcctg tacaggggac cggggccggt
     9901 cgtgaccgta tcgatcgtta cggggaaggc ccctcgaagc cgcgccgcgg cggtttctac
     9961 ggcggccgtc gtcttgaagg cccctacctg tatgtaatgt ctccccggaa tgaggctcgt
    10021 ggagtccggg gcggcggcag gctccttcgg aggagacgac ggtttcacgg gaaccggggc
    10081 ggcggcgacc ggggccgcgg gggccgcggc cggcttggtc ggagcctccg ggggcttggg
    10141 agccgcgggt tccatcgtga cgacgacatc cgccggttcg gccggcacgg aatccgcggg
    10201 cggtacgacc ggcgagggct cgggctgctt ctcaggttcc gttttggcca ccgcgatcgg
    10261 ttccgcgagg ccgacttccg gcgctcgatc cgaatcggga gagccggtct ggctccgcga
    10321 cagggcttcc ggctttccgg agtcatccat gcgtatttcc aggtccgcca gcgccacgct
    10381 cggcgccgga tccgccggcc aggccttccg ggtcagggca gtctccggaa gctcgtccgg
    10441 taccagcacc gggtccgcca gggcggcctc cacggggaga gccgcgggag cgggccgatt
    10501 cagtccgtgg accacggcct tgtcctcggg gacctcgcgg accgcggccg tttcgggagc
    10561 cggaaggacc gcggtgacgg gtccggactc caaggggacg acccccgggc gggcgatcaa
    10621 ttcgggccgg accacgggaa cttccttggc gctggggctt tcggggaccg gggcgggggg
    10681 aagagagggt tccggcgcct cggatggggc ggaaacctcc gggggctcgg tcggggcttt
    10741 cggcgatacc tcggcgttga ccaccgcgga cgggggcgga gcgaggttcg agggcaccgg
    10801 ttccgggatc gccggttcct ccgacgcggt tcctgcggcc cccggctcgg ccgcaaccgc
    10861 gggcacctca acggggatcg tagatcgtgg ctcctcggta ggagtggata ccccggcccg
    10921 ggaggccagg aggctgggat tgaagtccgg atcaacgccg ggccccgagc ccgcaggcgg
    10981 agtctcggcg atgttgcggg gggcggatac ccgtacccgg gacagggagc ccggttccat
    11041 ggagagggct ttcgccgctt cgggagagag aagcaggaag atcccggggt tttccaagcc
    11101 cttggtgacg atcacggtca cggacttgcc gttctccagg ttcactacct ccacggccgt
    11161 gttcctgggg aaggaattgc aggccgcgta gtatccggaa tccgggaact ccccatacgc
    11221 gctcatcatg gcgcttcctt cccaggcgga tgcgcccaac aaggacgtcg cgagaagggc
    11281 gatacaaagc accggcatcc atcgtttcat attgtctctc ccgcggatat cacccgcgtt
    11341 ccttcctcaa ggtcctcgcc cacaccgccg ccaaggtttt ccccagctgg gaaaggcgtt
    11401 cccggcgttc ctcgaccgtc ccggaatcga accgttccgg cttctcggac ccctcggcca
    11461 gggcactgcc gtcccggacc ctcatgagcc tccaggcgag ggattccggg aaccgcaccg
    11521 gcgggtccga aggtgtatcc gcatagcgta tccagacgag ggcgacatag tccacatacc
    11581 cttcccgggc cgacttcaga cccaggacgc tcgactggaa gtccgcccgg ccgatccgtc
    11641 cgatcgtctc gttggtggcg atgaatccgg ccgcgaagag atggtccagg cagccgccca
    11701 atagggcgtc ggacaggtcc ggggcttccg gtccccccga gtcctcgagc agcgcgaccg
    11761 agaaggtctg tcccgacagg ggcgcggcca ggcagaggac ggcaagtacg caagccgata
    11821 cggttcgctt cacggatact cctctccgcc ttccaatatc ggcgttccgg cgtgccgcgc
    11881 ttaccctttt ttccacgttg aaccggatcg tccgccgtgg tattgtgcgc tccagggagc
    11941 cggagggatg cagtactacg cggttcaagt gaaatcccga tgcgaggacg agtatgtccg
    12001 caggctcagg tcgaatcccg ccgtttccgg ggcggagatc ctcctcccga agcgcacggt
    12061 gatccttcgc aagggcggta tcaagcggcg gggaacggcc cccgtattcc cgggctacgt
    12121 cttcctgggg ctcgacgagg cggtgaaccc ggaactccgc tggctcgtcc gcaggactcc
    12181 ggatttctac cgattcctgc cttccaatca ggatcccgtt cccctgggcg ggcgggacct
    12241 ggacctgctc acgcacttca tttccttcgg ggaacgtgcc gatacctcaa aggtagcctt
    12301 cgacgagaac gaccgaatcg tcgtgctgga aggccccctg aaaggcctgg aaggcctgat
    12361 cgtgaaggtg gaccgccgga aaggccgggc caaggtccgg ctggacatgt gccggaactc
    12421 cttcctgatc gacctggcct tttccgtcgt ggaccgcgcg gggaaaggac aaacggaacc
    12481 ccatggagaa tcgcgaggaa cctaggatct acgtgatcgg cgcgggcttc gccggtcaat
    12541 ccatcgcccg ggaaatcaag gcccggggga tactgggccg ggtcgtcgcc ttcctggacg
    12601 acgatcccga gaagatcgga cggcgcctgg aaaacgtgcc ggtcctggga ccgatccggg
    12661 acgtggtgaa tctcctgcgg cgcacccccg cggacgaggc catcatcgcc atgcccagcg
    12721 cttcccggga ttacctccgg gagctgtacg gattgttgaa ggccgcgggg ttttcccgca
    12781 tccgcatcct tcccggcata gcccagatcg tggaggggga cgcccacctc atccaggccc
    12841 gggagatcga tccccaggac ctcctgggcc ggggtcccgt ggtcatcggc ctggcggaga
    12901 gcctttccta cctcaaggaa aagcgggtcc tggtcaccgg ggccgggggc tccatcggct
    12961 ccgaactggc ccgccagctc ctttccgccc gggtccagag gctctacctc ttcggccacg
    13021 gggagaactc catctaccag atcgataagg aactccgcct gctccaggct ggcggggtcg
    13081 gggtccggac ggacatcgtt cccgtcatcg gggagctcaa ggaccgggcc tacacgaccc
    13141 atatcctgaa acgtctcaag gcggatgtga tcttccacac ggcggcctac aagcacgttc
    13201 ccatgatgga ggagaatccg gtcgccgcgg tggagaacaa cgtcttcggg acctggaacc
    13261 tggcggaggc cgcccgggaa gccggaaccc agcgcttcgt gctgatctcc acggacaagg
    13321 ccgtggatcc cgtctgcgtg tacggggcct ccaagctgat ctccgaacgg atcgtccggg
    13381 aggcccagaa gaaggtcggt gcaggacgga aattcatggt ggtccggttc gggaacgtcc
    13441 tgggatcccg gggttccatc cttcccctgt tccagcggca gatcgagacg ggaggccccg
    13501 taacggtcac ggatccggaa gccagccgct atttcatgac catccccgaa gcctgctccc
    13561 tggtgctcaa gacggggggc gtgggcgagg gcggggaatc ctacctcctg gacatggggg
    13621 aacccgtccg gatcaaggac ctggcggagc agctgatccg gttctacggc ttcgagcccg
    13681 agcgtgacat ccccatccgg tacatcggac tgcggcctgg ggaacggacg gtcgagaggc
    13741 tgcacagcga acgggaaacc ctcatcccca cgaccttccc gagggtgaat cgcctggtgc
    13801 cggactccga tacggacctt ccggtggacc gactcctgga ggaactccgg cccgtctgcg
    13861 cgcccctgga aggtcgggag ggggagtacc ggaaccggcg caggctacgc cagatcctga
    13921 ggacctacat accgaccgtg gaggccccgg cccatgaacc cgagtactga tttcgtcccc
    13981 ttcgccctgc cctccctggg accggaggag gaggaggccg tccttgcggt tctccgttcc
    14041 ggatggctga ccacgggatc cgtggccctc cagttcgaga gggagttcgc ctccttcgtc
    14101 ggggcggagc acgccctggc ggtgaactcg gcgaccagcg gcctgcacct ggccctggaa
    14161 gccctgggcg tcggccccgg ggacaccgtg gtcacgagcc cctatacctt cgcctccacg
    14221 gccgcggtag cccggcacct gggcgcggag gtggtcttct gcgacgtggt ccccgggggc
    14281 tacaacatgg acccccggcg gctcgaggag gtgctcgcgt ccacccggaa cgtgaaggcc
    14341 gtagtgccgg tccacgtcgg cggactgccc tgttccatgc cggaaatcct ggacctggcc
    14401 cgcaggtacg gatgcgcggt tgtcgaggac gccgcccatt ccttcccctc ccgccttccg
    14461 ggcggcttcg cggggaccct gggggatatc ggggtcttct cgttctacgc caccaagacc
    14521 atcaccaccg gagagggcgg catggtcgtg acctctgatt cccgcctggc caagcggatg
    14581 tccgtcatgc gcatgcacgg aatcgacaag cccgtctggg accgctacac ctcgaagacc
    14641 gcgtcctgga gctattccgt cgtcgaggcg gggttcaagt acaacctccc ggacctcctg
    14701 gcggccattg gccgggaaca gctcaagaag gccgaccggt tcctggagga gaggcgccgg
    14761 atcgccggga tctacggcga aagcttccgc ggcctaccct tcgcggagct tcctccggag
    14821 ggccccggcc acgcctggca cctgtattcc ctccgcctca ggccggggag cctgcaggcg
    14881 tcccgggacg aattcgtgga tcggctccga gaggcgggga tcgggacctc ggtgcacttc
    14941 atccccctcc ataccatgcc ctattgggcg gagcgatacg ggcttacccc ggacaggttc
    15001 ccggaagctc tttcctcctt ccaacggacc gtctccctgc ccatctggca gggcatgggc
    15061 gaggaccgcg cccgcagggt ggcacgggcc gtagcagaga cgggcgccgg gctgtcgggc
    15121 taggagcagc atgccgcggg accggaacga atcgctgagc gccgatatcc ccctgtccga
    15181 tcttcgcgac gaggggatga tccgggtcgt caccgtccgc gccccggtct gtcggggacg
    15241 catcacggac atcgaggtcc cccgcctgcc ccggggatac cgggccatca gggccgagga
    15301 tatccctgga gagaaccgcc tggccatcct gggcttcgac attcccatcc tggcccggga
    15361 tcgggtcgcc tacgccggag agcccgtcct gctcctggcg ggaccggacc cggccgtcct
    15421 cgaagacttg cggagcacgg tccgggtatc cgtccaggag gaggagcccg tcctcgagtg
    15481 ggagtccttc tccccggagc aggtgatcca gcggcggagc gccgtgctgg ggaaccccga
    15541 gaaggccttc gaagccgcgg cggaggtcct ggaagcccgg taccggacgg attcccagga
    15601 gcacctctac ccggagcccc agggagccct ggcggtcttc gactacgaca agctcgtcat
    15661 ccgctgcgcc acccagtggc ccttccacgt tcgggactgc gtgcggtccg tcctgggggt
    15721 caagccccag gaggtcatcg tccgcccgac ccccctgggg atccccatgg agggaaggct
    15781 ctggtaccct tcggtcctgg cctgccacgc cgccctggcc gccgtcctgt gccggaagcc
    15841 cgcccgcatc ctcctgtccc gggacgagga tttccggttc accccgaaga aagcccgctc
    15901 cctcgttacc ctgcgcgcgg gtctcgaccc ctcgggcacc ctgacggtcc tggaggccgt
    15961 agtcgtcctg aacaccgggg cgtacgctcc cctcgcctcg gagcttctgg aacaggcctg
    16021 ccttcagatc acgggatccc tttccatacc gaaccttcga gtccagggct acgccgtacg
    16081 gaccaacacc cccccccttg gcgcgttttc cgggatggga gccgcccatt cgctcttcgc
    16141 cgcggagagc ctggccagcc ggatagcccg ggaccgggag gaggatcccc tggaatggag
    16201 ggcccgccac ttcctccgga agggatcccc cctgctgacc ggggaacggc ttcgggacag
    16261 gaccaccttc gaggagatag gctcccggct caaggcctcc agcgatttcg cccgtaaaca
    16321 cggcagcaac gaactcatcc gcaagcggcg atccgcctcc cgaagcgagg aagccccccg
    16381 gggtatcggg atcgcgttcg ggtaccaggc caacggcgcg tcctacgccg ccctggcctc
    16441 cggcaccttc acggtggaga ccaccctgaa caaggacctt tccctggtga tctccaccag
    16501 cgcgatagcg ggcgacgagg gtatccgatc catctggcgc tccaccgccg ccggcatcct
    16561 gggcgttccc ctgggcaggg tatccatctc cgaggtggat accgcctcgg tgcccgattc
    16621 cggcccgtcc atgctttccc gcaacatctc ggtcgtgaat cgcctgatcg aacggagctg
    16681 tatcgccatt cgaagccgaa ggttccggtc tcccctgccc atcgcctccc gggccgtgca
    16741 tcgccccagg aatccgctgc gctgggagga ggatgcggtc cggggttatc ccttcgagtc
    16801 cctttcctgc tgcggggcgg tggtggaact ggaattggat acctggagca tggaaccccg
    16861 catcctgggg gtctggctct gcgtggacgg cggacggatc gcctccgagc gattcgcccg
    16921 gggcaccctg tcctcgtcca ccctggcggc cctgggccag tgtttccggg aacgcctgcc
    16981 cttcgaggag gggcgtgtgg ccgagaacgg gtaccgggaa tacgggcttc tgtccctggc
    17041 ggaagccccg cccatcgaga tcgagttcct tccgtggagc gatacggatc ccttgaaggg
    17101 aatcggggaa ctgcccttcc tctgcatccc cgcggcattc gcctccgcgg ccgcccaggc
    17161 ttccgacctg ggcggggaaa gccttccctt cgacggcgcg gagtaccttg ccgggatgga
    17221 agccgtatga agctggattt catcctgaac ggggaggacg tctcctgcca ggcggaggcg
    17281 ggggaccggc tggcctacat cctgcgggag cggttttccc ttctcggggc gaaggaggat
    17341 tgccggtccg gccattgcgg cctgtgcatg gtaagcctga acggccgggc ggtcccctcc
    17401 tgcctggtcc cggccttccg ggtccgggga gccgaggtcg tcacgatcga gggattcgaa
    17461 ctgaccgacg actacgcgga tctccgggac ggattccgta aggccggggc ggagccctgc
    17521 gaattctgcc ggggagccgt cttcttgtcc gccgccgccc tgctggatga gcgtccgcgt
    17581 ccagaggcca ccgagatcct ggagcacctg tccgcggtgt tctgccgatg tcacgacccc
    17641 tcggccctgg ttcggggagt ccagaccgcc gcggaactgc gggcccggag gatgtatttc
    17701 cgtgcgaatc gatgaggtga tcgttccggc caccttgagc gaagccctct cggcgctcaa
    17761 gaggaacccc gatgccgttc tctgcgcggg gggcacggag ctcttttccg aaggaccggc
    17821 cggaccggcc gaccgtaatc ggacgctgat ttccctccat gggattcagg aactccggca
    17881 tgtctcccgc acggacctct acgtcgaggt cggatccatg accaccctgg cggagcttct
    17941 cgcgttgaag gaaggcttcg tgccggaaac cctccggtcc gtggtacacg ggataggaac
    18001 cttcgcggtg cggaacctcg ccaccctcgg gggcaacctg tcggtcacgg atcggttccg
    18061 ggactgcttt ccgatcctgg cttgcatgga cgccctcgcg gaataccggc agggaaccgc
    18121 ttcgcgctgg gtgaacctga accgcctgat cggtccccgg gggaccccgg aaatgccccg
    18181 gggggagatc ctcacgagaa tccggattcc cctgggagga tgggacctcg ggatcgccca
    18241 caagctcggg ttcccgagga tcaactcccg aaagtccagc cttttcgtag ccctggcccg
    18301 cacggacaaa cgggcccttt ccgacatacg catcctctac gcctgcgacc gggccctgag
    18361 aagccgggaa atcgaatctt ccctcgccgg gaagaagatc cccctcttgc cccgggatat
    18421 agaatccgcc gtcggtgcct acgcggacta ttgttcccac gcgggcatcc gggaatcctc
    18481 ggccgcccga ttcctgacct tggtgcgcag ggctttcaac cagctcaacg agggggtgac
    18541 ggcatgaagg ccccgggccg cctcttcctc ctgcccgccc cgatcgggga cggcgatccc
    18601 ctggcaaccc tctctccgga ggtcatcgag atataccgga gcctgcggca cttcgtggtc
    18661 gaatcggaga ggaccgcagg ccgcctcctc tcccggatcc tgacacccga atccctccag
    18721 gagaccgcgt tgaataccct gagcgagcat acggatccaa aagccatacc gggcctcctg
    18781 gaaccggccc tggagggacg ggatctggga attctctcgg aggcgggctg cccctgtgtc
//
LOCUS       DAOTEI010000056        18513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_1994_length_18513_cov_5.600065, whole genome shotgun sequence.
ACCESSION   DAOTEI010000056 DAOTEI010000000
VERSION     DAOTEI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1994_length_18513_cov_5.600065"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1000)
                     /gene="arcC"
                     /locus_tag="P5313_07310"
     CDS             complement(59..1000)
                     /gene="arcC"
                     /locus_tag="P5313_07310"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRY80206.1"
                     /translation="MSKKTIVVALGGNALIEEGTEGTMEQQFENTRKSMAAIVGLIQE
                     GHRVVLTHGNGPQAGVHLIRNEAASAKVPPSSLNVIVADTQGSIGYMIQQSLVNALIK
                     AGVEKSVVTVVTQVVVDKNDPSMLNPTKYVGPFIRPEQVEECRAKGWVVKEDPGRGHR
                     RVVASPIPTGIVETEVIRDLLHDGLVVIAVGGGGIPVCRNADGTYEGVDAVIDKDRAS
                     ALLANLLEADELLILTGVEKVAVNFRKPDQKVLDVMTVAEAEKYMAEGQFPKGSMGPK
                     IEAAVDFIKRGGSQVVITDLEHAQAGAAGKAGTRVVK"
     gene            1156..2022
                     /gene="ispH"
                     /locus_tag="P5313_07315"
     CDS             1156..2022
                     /gene="ispH"
                     /locus_tag="P5313_07315"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRY80207.1"
                     /translation="MIVLRADTLGYCMGVRRAMDLAVRTAETEAGRPIYTLGPLIHNP
                     QALEDLRRRGVEVLPEGEADARIAGRTVILRAHGVSPELRTRLETLGARLSDATCPRV
                     LASQRRARRFHEAGYSVVLVGDRNHGEIVAVTGCAPGCSVVGSPAEAEALDLAEPVGV
                     LAQTTVTEEEYDAVCRVLRARYSRLAIVDTICPATRERQESLARLAARAEAIVIVGGR
                     SSANTARLFRAARALGRPAWHIETARELPPEVFGFGVVGLTAGASTPDSIIAEVEEAL
                     LDPGRAGRKAEG"
     gene            2024..3769
                     /locus_tag="P5313_07320"
     CDS             2024..3769
                     /locus_tag="P5313_07320"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRY80208.1"
                     /translation="MPGNGGRPRFRGGYFAHIFRLFLAAAIAPALAVSLVYSSTAGAA
                     LRREAESRARADAASFAAAVGEIADRAAESLALLAADPDIQSLLSETSADPARSARAA
                     RRVQTLFSRDNREAEGSVLGLRGSPTLTPGGAAADRDPEVYGSWGLFRLLRERPGDLA
                     VLGRRGTAEDGETTILSLARTVAAPGGEVLGYAVADLRRSALVRAAERSRIPAGAEVL
                     DPRGNVVFDLADPGREGYFEDELAPWNSGGGPAPEAPIPGAASGYRVRIALPAGLYDG
                     FDRTARTVAVLGLLGSVLVAAALAFPASRAVTGPVLRLARSMREVEEGDLSIRVEPSG
                     NDELGDLARSFNAMTEELAELLRNAVERQELLREAELRALAAQMNPHFLHNTLAAIKS
                     LAKLGRNTEAAEAAARLGKILRAGASRREEFTTVGESLDLVRDYLAVERIRFGDRFRF
                     RIEVDPALLAVPIPPLVLEPLAENALTHGLERKRGTGTLRIEGGLEGEDALISVEDDG
                     PGAGAERLAGLRRILAEGSLPDSDHGMGLAGTNRRLRLEYGPGYGVSVHPGPDGTGGF
                     RAVVRIPARVREAAR"
     gene            3766..4575
                     /locus_tag="P5313_07325"
     CDS             3766..4575
                     /locus_tag="P5313_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY80209.1"
                     /translation="MRRLLISEDEELFRRELETTVPWADWGFVLVGAAEDGAQAWDLL
                     RSRRAEAVLTDVRMPLVDGIELMRKASDLPLPERPLFVIVSGYADFRYAQEAVRLGAF
                     DYLLKPVEDEALEGTMRRAAAELESRDARDGLLRSSASDPVLAFFRNYLPGRTREPGD
                     AYVEAAVEEISARYLSDLTVDAAAGRLGISGDHLARIFKRRTGYTFNEYLTRFRIQRA
                     VELLRDPSVRVGEVADLCGYRDPRYFSVLFRRLVGMTPSEFRSGRGRPPSR"
     gene            complement(4611..5636)
                     /locus_tag="P5313_07330"
     CDS             complement(4611..5636)
                     /locus_tag="P5313_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY80210.1"
                     /translation="MRPGSAFALAAAIAAALASCGPRAPEPAPEAPADLVVYSSHPAE
                     LVRAVVREFRERTGLRIRVVSGGTGELLARLRSGEQAYQADLVWGGGAESMAAASDLL
                     EPYVSPEAASIPPELKDPDGAWTGFTVLPMVIIVNERLLPADRAPRSWGDLGSEALRG
                     SVAYADPSVSGSAYTILRTLLLAGGGGEAGWSRIAAFARTLEGNVLPESSQVFRGVAS
                     GEYLAGLTFETAVTDAGRSGSDVRVVYPEDGTSAIPDCVALVRGAPHPEAARAFVDFV
                     LGPDVASVVVGRFGRRSARGDAPAPEGLPPLSSLRILPYDLESSAAERAATLARFRDL
                     LAGAARP"
     gene            complement(5633..6235)
                     /locus_tag="P5313_07335"
     CDS             complement(5633..6235)
                     /locus_tag="P5313_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY80211.1"
                     /translation="MIDLFVFDLGGVLVHDFDIIPEAARRLGLAEGRMRDLAHPDMED
                     YLSGRIGPGEFWERFRIRSGLRVPENYWATLFRPVLDPDMEALVLSLKSSGRVVCGTN
                     TIDVHYEWLRERGFYSCFDAVYASQLLGVSKPRGEFWLRILEAEGRDPGRAFFTDDME
                     ENVEAAGALGIRSHRFEGIGGLVSALEEAGIPVPVERARP"
     gene            complement(6232..7038)
                     /locus_tag="P5313_07340"
     CDS             complement(6232..7038)
                     /locus_tag="P5313_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY80212.1"
                     /translation="MAFLAAACLLVLVPLAFMAGASFMPALDIMKMPYRWIPETWKPA
                     NYLRALAGNDGNWMYLRNILNSLVVSSSIAVSTVFLSSLVGYGLAKFRFKGNQVVFLL
                     IMATMMIPFETVMIPLYMIIVKLGLQDSYAGLIVPFLMNAFGVFLMRQYLITFPGEIL
                     DAARIDGCSEPRIFREIVFRNAGPAAATLAVLSFRQQWDNLLWPLLVAQREEMKTIPA
                     YIVKFAAEKHADEGAMMAVAVIASLPVFALFFGLSKYFLGGSAVYSAGKE"
     gene            complement(7092..8003)
                     /locus_tag="P5313_07345"
     CDS             complement(7092..8003)
                     /locus_tag="P5313_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY80213.1"
                     /translation="MKPAVTSGVLERNRSRWGWAFTAPGLVFFSIFSFYPILNAIWTS
                     FFNKRLLSLRPPKFVGFQNYARVLGSEDFWNSVRATAAFTAGVFVPLFTLSLLLAVFI
                     ASRKRFRRLFQMALYSPAVLSSVVAALIWLLLLDPRGLANQFVNLVARTPGVDRRWLS
                     DGTMVQVSTMMVYVWKYVGYFTILFITGIGKIPESVYEAAVIDGAGPVQRFFRITMPL
                     LRPTTVLVSVMILIQCLKTFSTQYLFTQAGAPVGPVNVITLNIYNTAMRDLNLGRASV
                     MSTLLFLFMLGLTWVQLRFSRRDEVEL"
     gene            complement(8259..9557)
                     /locus_tag="P5313_07350"
     CDS             complement(8259..9557)
                     /locus_tag="P5313_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY80214.1"
                     /translation="MKRILTAILVIALSLPVLAQAPAVPELKGKSVELTIWTHEDPAR
                     TALEKRLIAEFSAANPGVKVNYVTYPSSKIKDIVVAGFSANQGPDIFNLEIFDEYQFI
                     VNGRVAPVDLKAAGYKSARDLQGKYLPGMLDPVTEGGKVYGLPLELTNWCIYLNKKIF
                     RDAGLDPDKDWPKTWEDVVAVSEKIVKRDGQIITRRGFDFRYGYYLTSVLPMVEQLGG
                     ELISKDGKKAILGDAAWIKVLTYFRDFGPNGKNLGSPTYTDARRIFDNDKNEIAMSLS
                     GLYQEARMRGANPAFFNSSDWMVIPFPQWKDAVKKVPNNYYGHYYMVNAQKPKLSQQA
                     SWALVSYMLSHGAEYLEKVAIVQPTKALMESATFKNMPYSDVFAKDMAAGHIVYYGAS
                     SSKIEALLKEAFTSVMLSGVEPAKALEKLRKDVTAALADE"
     gene            9801..10364
                     /locus_tag="P5313_07355"
     CDS             9801..10364
                     /locus_tag="P5313_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY80215.1"
                     /translation="MTLMKEIEERRAYRALSPDPLPEDQVERLLTAAHHAPSCFNKQS
                     WRFVAVSGDALAGLRETLSEGNKWATRAPLIVAAAQKPSLDCRLDEGRDYAPFDLGLA
                     VMSLVLQATHEGLVAHPIAGYSPGKVRKALGIPEDYVVHTLVIVGGKGSDELLSDWQK
                     ERETGPRLREPLEKVAFRNRWPDPVSG"
     gene            10542..11162
                     /gene="udk"
                     /locus_tag="P5313_07360"
     CDS             10542..11162
                     /gene="udk"
                     /locus_tag="P5313_07360"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRY80216.1"
                     /translation="MATAMIIGICGGSGAGKTTIVRKISEVVSDFVFIPQDNYYKSAE
                     YISNSNITAFNFDHPEAFDNELLSAHLRELKAMRPIEMPTYDFVHHRRAEQTIKVTPR
                     KLVIFEGIMVFTDKVIRDLIDLKIFVDTPDDIRFIRRLGRDIKERGRTVDSVIEQYLN
                     VVRPGHFEFIEPTKRFADIIIPEGGANENALTVLLSFMRNIIDPRP"
     gene            11187..12581
                     /locus_tag="P5313_07365"
     CDS             11187..12581
                     /locus_tag="P5313_07365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY80217.1"
                     /translation="MVLQQALRLSAWTSTFSIAALFAAILVFAAPGLPFLPTTLLGAG
                     SFVVLFLIQDALVRALDSDLRKRHFDRAETRIVMDFVERLRVSFTVADLVEAARDCLE
                     MRADASVLLIKSATWETVYASPSTLAKDPETLAILHRNFREWSEGFAFLSDEYGLHPS
                     NAGCRGYLSVCKGYHLFVFSRSAGLAELEAFRLLHGELQLYFDRIITVARLFNIANLS
                     KEWSLIAETQKSFLPKEMPKLAKLETASYYRPLVNVSGDYYDVIPVDYRRTLLVVGDV
                     SGKGLAAALIMGIIVNTIRVAPDKTDLPALVRNVDEAIREMRFEDKYTVIFLGIVDTG
                     EKVLRYVNASMADPLVISQTALGAKPKKLGSTMGLLGLLPLEGEIPVEEIPLRTDDVI
                     LIASDGLLELENSAGEPLSESAAFERLLQQSSRETAESIVEGIAGFAYSFVEGRQLRD
                     DITILAAKVGRLWD"
     gene            12572..14722
                     /locus_tag="P5313_07370"
     CDS             12572..14722
                     /locus_tag="P5313_07370"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRY80218.1"
                     /translation="MGLTIFAFLAPPILLGLWARARTRIRDTAWSRYFLAIGIGFSVF
                     TLSLGVAYLAAQPYTLEQPLALFFTRLAAASAAAGYIVLFRVCLSFPYETRNRIADLA
                     AAAAVLGVLALTLGTPLYVSHVERVNLDFLRQEGPLYGAISTGAAALGFLGAAVLLFR
                     SLAMRSRIFQQQGLILALGIAASIVLGYVTAIRLPQVGRIGSLYLVSTLSPVVVAVGF
                     FYAVSTSRIFAWKEVARRLVSYLILAILAGTPVGVAVSVFLMVKAFPLISFLGAFFSF
                     ILGARIASAFVNRFLDSIAGRKRYREDLQSSLAHVDLAGGRDAVLKEVHGILSGPLGF
                     SDFTVLAEDDEGELRPVYSTTGTSLSIRGDSAALGTLTERGITVLLKTDVIANRSYAS
                     VKKELLEIYDSLRAEAMIVVREGRETLGIFALGARLSGGDYTSYDYETFRAVYGKLFV
                     VLYYVKNIARESILSTVDREIAFSSQVIESIQENVDRIAHPKADAHFIARSTRKLGGD
                     FIDFVGLGRDRFFFVVGDVSGKGLNASMSMLILKTMVRNFLKEEKDFLRIAERANSFI
                     KENLPKGTFFAGVFGYFDFAKDSLYFINCGIPVMQMYSPAFNTVIEIQGDGRVLGFVR
                     DIRPYLKLRRVPLVKGSALLISTDGILESESLRGERYGKERLQRSFTANREGPAKELA
                     EGLLRDLLAFTDNRMEDDVTIFTLKYDTNVRSEA"
     gene            14719..15045
                     /locus_tag="P5313_07375"
     CDS             14719..15045
                     /locus_tag="P5313_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY80219.1"
                     /translation="MNQLTVEEIRETGRTILQIAGPVNSYTFTDFQEKIFKAVRRSDI
                     VVDMEKVTTLSSAGLGVLMAAMEDAEGAGRKIWILRPSEIVRLAIESTGFSDRFPVAQ
                     SMKDLA"
     gene            15081..15497
                     /locus_tag="P5313_07380"
     CDS             15081..15497
                     /locus_tag="P5313_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80220.1"
                     /translation="MTDPSKSPGFSTRLPGDRSAIPRFEDWLDRAAIPDPRLRDRVRL
                     AGLEILDNLIRHSGPLEGGSVRTSLRLRAGGARLVFSFRSVKFRSFARGGKNPRTGYN
                     IEDGRWRGLGLVMVAFLTLHVRYVSDARSDRVEAEF"
     gene            15711..16754
                     /locus_tag="P5313_07385"
     CDS             15711..16754
                     /locus_tag="P5313_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY80221.1"
                     /translation="MVVLAIAGCAKKEAPAAAPSLKVGMVTDAGTIDDKSFNQGTWEG
                     VVRAGTEFKLDTKYLKPSGTTEADYLKEIGNLYDAGYKFVVTPGFKFETTIFQAQTKY
                     ADAKFVLIDGSPHAGDWKSVVGPNTVSIFFAEHESAFVAAVAAALQIKTGSFGFIGGM
                     EIPPVQKFNWGFQQGVDYANKNLGTKITMKAENVIYQGSFDNVAAGQQIAASMYDKGV
                     SVIFAAAGGVGVGAINEAKARATAGKTAWIVGVDVDQYAEGIYSGDKSIILTSAMKGI
                     DVAAFDMIKAELGGTFPGGKTLIFDAKNNGVGIPAKNPNLSPEVQAKVAEVFKLLQAG
                     TIAVKAEQGTLIK"
     gene            16968..18494
                     /locus_tag="P5313_07390"
     CDS             16968..18494
                     /locus_tag="P5313_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80222.1"
                     /translation="MEYIVEMLGITKDFPGIRANDDITVRLRKGEIHALLGENGAGKS
                     TLMSILFGLYRPDSGIIKVRGKEVSIHNPNVANALGIGMVHQHFKLVHNFTVTENIIL
                     GMEPTAGPMLQTRDAAKRIKALSEQYGLNVDPDAKIEDISVGMQQRVEIIKMLYRDAE
                     VLIFDEPTAVLTPQEIDDLMQIMRNLLKEGKSILLITHKLKEIKAIADRCTVIRRGKV
                     VDTVDVADVAESRMAEMMVGRTVNFKVDKREQERGEAVLSIRNLNVLSSRKTQALKNF
                     SLDVHRGEILGLAGVDGNGQTELVEALTGLRKVESGSILFKGRDITHDDIHSRVMAGM
                     AHIPEDRQKRGLVLDYSMEDNFILEIYGLPPYSRKGFLNRKAIRDHAEKLIQDFDVRS
                     GQGALSVGRTLSGGNQQKAIVGREIDHDPDLLLAVQPTRGLDVGSIEYIHKRLVEQRN
                     NGKAVLLVSLELDEILDLSDRIAVISHGELTGVKLPKETNESEIGLMMAGIAGAGGME
                     "
BASE COUNT         3121 a         6248 c         6149 g         2995 t
ORIGIN      
        1 cattcaccca ttcacccatt cacccattca cccattcacc cattcaccca ttcacttctt
       61 acttcaccac cctcgtcccc gccttgccgg ccgcgccggc ctgggcgtgc tccaggtccg
      121 tgatgaccac ctgggagcct ccgcgcttta tgaagtccac ggccgcctcg atcttggggc
      181 ccatggagcc cttggggaac tggccttcgg ccatgtactt ctccgcctcc gcgacggtca
      241 tgacgtccag gaccttctgg tcgggcttgc ggaagttgac ggccaccttc tccaccccgg
      301 tcaggatgag gagttcgtcc gcctcgagga ggttcgcgag cagggcgctg gcccggtcct
      361 tgtcgatcac ggcgtccacg ccctcgtagg tgccgtcggc gttcctgcac accgggatgc
      421 cgccgccgcc caccgcgatc accaccaggc cgtcgtgaag gaggtcccgt atgacctcgg
      481 tctcgacgat gcccgtcggt atgggggagg cgaccacgcg ccggtgtccc cggcccggat
      541 cctccttgac cacccagccc ttggcccggc actcctcgac ctgctcggga cggatgaagg
      601 gccccacgta cttggtcggg ttgagcatgg aggggtcgtt cttgtccacc accacctggg
      661 tgaccacggt cacgacgctc ttttcgaccc ccgccttgat cagggcgttc accagggact
      721 gctggatcat gtacccgatg gagccctggg tgtccgcgac gatgacgttc agggaactcg
      781 ggggtacctt ggcggaggcc gcctcgttgc ggatcaggtg caccccggcc tgggggccgt
      841 tcccgtgggt caggaccacc cggtggcctt cctggatcag tcccacgatg gcggccatgc
      901 tcttgcgggt gttctcgaac tgctgctcca tcgtgccctc ggtgccctct tcgatcaggg
      961 cgttgcctcc cagggccacg acgatggtct tcttgctcat agtaccttcc ttttttgggc
     1021 gcgagccacg ccggatagag aaacggaggg tcagtatata ggttcttacg gttccgaaca
     1081 atataccgcc ggatcccggg ccggatcccg gcggcccgct gggctgtccg ggcgatttcg
     1141 cggtatcatg gaaccatgat cgtcctgaga gccgacaccc tggggtactg catgggcgta
     1201 cgccgagcca tggacctggc cgtccggacc gcggaaacgg aagccggccg tcccatctac
     1261 accctgggtc ccctcatcca caatccccag gccctggagg acctccggcg ccggggggtg
     1321 gaagtgcttc cggaggggga ggcggacgcc cggatcgcgg gccgcaccgt catcctgcgg
     1381 gcccacgggg tctccccgga gctccggaca cgcctggaaa ccctgggcgc ccgtctttcg
     1441 gacgccacct gcccccgggt cctggccagc cagcgccggg cccgtcgttt ccacgaagcc
     1501 ggctactccg tggtcctggt gggggaccgg aaccacgggg agatcgtggc cgtgaccggc
     1561 tgcgccccgg gctgctccgt ggtgggatcc cccgccgaag cggaagccct ggacctcgcc
     1621 gaacccgtgg gcgtcctggc ccagaccacg gtgaccgagg aggaatacga cgcggtctgc
     1681 cgggtcctcc gggcccggta ctcccgcctt gcgatcgtgg acaccatctg cccggccacc
     1741 cgggagcgcc aggagtccct ggcccgcctg gccgcccggg cggaggcgat cgtgatcgtg
     1801 ggaggccggt cctccgccaa caccgcccgc ctgttccggg cggcccgggc cctgggccgc
     1861 cccgcgtggc acatcgagac cgcccgggag ctcccccccg aggtcttcgg attcggcgtc
     1921 gtgggactga ccgcgggggc cagcaccccg gactcgatca tagccgaggt ggaggaagcc
     1981 ttgctggatc cgggccgggc cgggcggaaa gcggagggct aggatgccgg ggaacggcgg
     2041 acggccccga ttccggggcg ggtatttcgc ccacatcttc cgtctcttcc tggccgcggc
     2101 catcgccccg gcccttgcgg tttccctggt ctactcctcc accgccggcg cggccctccg
     2161 gcgggaggcg gaatcccggg cgcgcgccga cgcggcctcc ttcgcggccg cggtggggga
     2221 gatcgcggac cgggccgcgg aaagcctggc cctgctcgcc gcggacccgg acatccagtc
     2281 cctcctctcg gaaacctccg cggatccggc ccgctcggcc cgggccgccc ggcgcgtcca
     2341 gaccctcttc tcccgggaca accgggaggc ggagggctcg gtcctggggc tccggggttc
     2401 cccgaccctc acgcccggag gggccgcggc ggaccgcgac ccggaggtct acgggtcctg
     2461 ggggctcttc cgcctgctcc gggagcggcc cggggacctg gccgtcctgg gccgcagggg
     2521 gaccgcggag gacggggaaa ccaccatcct gtccctggcc cggaccgtcg cggccccggg
     2581 aggcgaggtc ctggggtacg cggtcgccga cctccgccgc tcggccctgg tccgggcggc
     2641 ggagcggagc cgcattcccg ccggcgcgga ggtcctggat ccccgtggga acgtcgtctt
     2701 cgacctggcg gatcccgggc gggagggcta cttcgaggac gaactggctc cctggaattc
     2761 cgggggcgga ccggctccgg aagccccgat ccccggggcc gcctcgggat accgggtccg
     2821 gatcgccctg cccgcggggc tctacgacgg gttcgaccgg acggcccgga ccgtggcggt
     2881 cctgggcctc ctgggctccg tcctggtcgc cgccgccctc gcctttccgg catcccgggc
     2941 cgtgacgggt ccggtcctgc gcctggcccg gtccatgcgg gaggtggagg agggggacct
     3001 ctcgatccgg gtggaaccct ccgggaacga cgaactcggg gacctggccc ggtccttcaa
     3061 cgccatgacg gaggagctgg ccgagctcct ccggaacgcc gtggagcgtc aggagctcct
     3121 acgggaggcc gagctccggg ccctggccgc ccagatgaac ccccacttcc tgcacaacac
     3181 cctggcggcc atcaagtccc tggccaagct ggggcggaac acggaggccg cggaggcggc
     3241 ggcccggctg ggcaagatcc tgcgggcggg agcttcccgg cgggaggagt tcacgaccgt
     3301 gggggagagc ctggacctgg tccgggacta cctggcggtg gagcggatcc gcttcgggga
     3361 ccggttccgg ttccggatcg aggtggatcc cgccctcctg gccgtcccca tcccccccct
     3421 ggtcctggag cccctggcgg agaacgccct gacccacggc ctcgagcgga aaagggggac
     3481 cgggaccctc cggatcgagg gcgggctcga gggcgaggac gcgttgatct ccgtggagga
     3541 tgacgggccc ggagccggag ccgagcgcct ggcggggcta agacggatcc tggcggaggg
     3601 ttccctgccg gactcggacc acggcatggg gctggccggg acgaaccgcc gattgcgcct
     3661 ggagtacggt cccggatacg gggtgagcgt gcacccgggc ccggacggga cgggaggctt
     3721 ccgggctgtc gtccggatcc ccgcgcgggt ccgggaggcg gcgcggtgag gcggctcctc
     3781 atctccgagg acgaggagct cttccgcagg gaactggaga ccacggtccc ctgggcggac
     3841 tggggtttcg tcctggtcgg ggccgccgag gacggggccc aggcctggga cctcctccgg
     3901 agccggaggg ccgaggcggt gctcacggac gtccggatgc ccctcgtgga cggcatcgag
     3961 ctcatgcgga aagcctcgga cctgcccctc ccggagcggc ccctcttcgt gatcgtctcg
     4021 gggtacgcgg acttccggta cgcccaggag gccgtccgcc tgggggcctt cgactacctg
     4081 ctcaaacccg tggaggacga ggccctggag ggcacgatgc ggagggccgc ggcggagctc
     4141 gagagccggg atgcccggga cgggctcctg cgatcctccg cctccgaccc ggtcctggcc
     4201 ttcttccgga actacctgcc cggccggacc cgggagcccg gggacgccta cgtggaggcg
     4261 gcggtggagg agatctccgc ccggtacctc tccgacctga ccgtggacgc cgcggcgggg
     4321 cgcctgggga tctcggggga ccacctggcc cggatcttca agcgccggac ggggtacacc
     4381 ttcaacgagt acctgacccg gttccgcatc cagcgggccg tggagctgct ccgggacccc
     4441 tcggtgcggg tcggcgaggt agcggacctc tgcgggtacc gggatccccg gtacttcagc
     4501 gtcctcttcc gccgcctggt cgggatgacc cccagcgagt tccgctccgg ccgaggccgg
     4561 cccccgagcc gttgaccccc ggacggattt ccgaccggga aatcaccgcc tcaggggcgg
     4621 gcggcgcccg cgaggaggtc ccggaaccgg gccagggtcg cggcgcgctc ggccgcggag
     4681 ctctccagat cgtagggcag gatacgcagg gacgacaggg gcggcaggcc ctccggggcg
     4741 ggggcgtccc cccgggccga cctgcgcccg aaccggccga ccacgacgga ggccacgtcg
     4801 ggacccagga cgaagtccac gaatgcccgg gcggcctcgg ggtgcggggc tccccggacc
     4861 agggccacgc agtccgggat ggcggaggtc ccgtcctcgg ggtacaccac ccgcacgtcc
     4921 gagcccgagc ggcccgcgtc cgtgaccgcc gtctcgaagg tgagcccggc gaggtactcc
     4981 ccggaggcca cgccccggaa gacctgggag gattccggga gcacgttccc ttccagggtc
     5041 cgggcgaagg cggcgatccg cgaccagccc gcctcccctc cccctcccgc caggagcagg
     5101 gtccggagga tcgtgtaggc gcttccggaa accgagggat ccgcgtaggc cacgctcccc
     5161 cggagggcct ccgagccgag atccccccag gaccgggggg cccggtccgc aggaaggagc
     5221 cgttcgttca cgatgatgac catgggcagg acggtgaatc ccgtccaggc gccgtcggga
     5281 tccttgagct ccggcggtat cgaggccgcc tcgggggaga cgtagggttc caggaggtcc
     5341 gaggcggccg ccatgctctc ggccccgccg ccccagacaa ggtccgcttg gtaagcctgt
     5401 tcgcccgagc gcaggcgggc caggagctcc ccggtgcccc cggagacgac ccggatccgg
     5461 aggccggtcc tttcccggaa ctcccggacg accgcccgga ccaactcggc ggggtgggac
     5521 gagtagacca ccaggtccgc cggggcttcc ggggccggct ccggcgcccg ggggccgcag
     5581 gaggccaggg cggccgcgat cgccgcggcg agggcgaacg cgctcccggg cctcacggcc
     5641 gagccctctc gacgggtacg ggaattccgg cctcctccag ggcggagacc agccctccga
     5701 tcccctcgaa gcggtgggac cggatcccca gagccccggc cgcctccacg ttctcctcca
     5761 tgtcgtccgt gaagaaggcc cggccggggt cccggccctc ggcctccagg atccggagcc
     5821 agaactcccc gcggggcttg gaaaccccca ggagctggga ggcgtagacg gcgtcgaagc
     5881 aggagtaaaa cccccgctcc cggagccact cgtaatggac gtcgatggtg ttggtcccgc
     5941 agacgacccg gccggagctt tttagggaga ggaccagtgc ctccatgtcg gggtcaagga
     6001 cgggacggaa gagggtggcc cagtagttct ccggaaccct cagtcccgac cggatccgga
     6061 agcgctccca gaactccccg gggccgatcc gtccggacag gtagtcctcc atgtccggat
     6121 gcgccaggtc ccgcatgcgg ccctccgcca ggccgaggcg gcgggcggcc tcggggatga
     6181 tgtcgaagtc gtgcacgagg accccgccca ggtcgaacac gaacaggtcg atcattcctt
     6241 gcccgccgaa tagaccgcgg agccccccag gaagtacttg gagagcccga agaagagggc
     6301 gaagaccgga aggctggcga tcaccgccac cgccatcatg gcgccctcgt cggcgtgctt
     6361 ttccgcggcg aacttcacga tgtaggccgg gatggtcttc atctcctccc gctgggccac
     6421 caggaggggc cacaggaggt tgtcccactg ctggcggaag gagagcaccg ccagggtcgc
     6481 ggccgcgggg ccggcgttcc ggaacacgat ctcccggaag atccggggct ccgagcaccc
     6541 gtcgatccgg gctgcgtcca ggatctcccc ggggaaggtg atcaggtact gacgcatcag
     6601 gaagaccccg aaggcgttca tcagaaaggg cacgatcagc cccgcgtagc tgtcctggag
     6661 gcccagcttc acgatgatca tgtacagggg gatcatcacc gtctcgaagg ggatcatcat
     6721 ggtggccatt ataaggagga atacgacctg gtttcccttg aaccggaact tggccaggcc
     6781 gtagcccacc agggaggaga ggaagaccgt ggatacggcg atgctggagg agacgacgag
     6841 ggaattcagg atgttccgca ggtacatcca gttcccgtcg ttgcccgcca gggcccgaag
     6901 gtagttcgcg ggtttccagg tctcgggtat ccagcggtag ggcatcttca tgatgtccag
     6961 ggcgggcatg aaggaggccc ccgccatgaa ggccaggggc accagcacca ggaggcacgc
     7021 ggcggccagg aaggccaggg cagccgcccc cccggcggcc tccgcgacgc gggagacccc
     7081 gacccgggcc ctcacagctc cacctcgtcc cggcgggaga accgaagctg gacccaggtg
     7141 aggcccagca tgaacaagaa caacaaggtg ctcatcacgc tggcccggcc caggttcaag
     7201 tcccgcatgg cggtgttgta gatgttcagg gtgatgacgt tcacgggtcc cacgggggca
     7261 ccggcctggg tgaagaggta ctgggtgctg aaggtcttca ggcactggat caggatcatg
     7321 acggagacca ggaccgtcgt gggccgcagg aggggcatcg tgatccggaa gaacctctgg
     7381 accggtcccg ccccgtcgat caccgcggcc tcgtacacgg actcggggat cttgccgatc
     7441 cccgtgatga acaggatcgt gaagtacccg acgtacttcc agacatagac catcatggtg
     7501 gacacctgga ccatggtccc gtcggacagc caccgccggt ccacacccgg ggtccgggct
     7561 accaggttga cgaactggtt ggccagcccc cggggatcca gcaggaggag ccagatcagg
     7621 gcggccacca cggaggaaag gacggccggg gaatagaggg ccatctggaa gagtctccgg
     7681 aaccgcttgc gagacgcgat gaagacagct aataggaggg agagtgtgaa caggggcacg
     7741 aagaccccgg cggtgaaggc ggcggtggcc cggacggagt tccagaagtc ctcggatccc
     7801 aggacccggg cgtagttctg gaaacccacg aacttgggcg gccgcaggga caggagccgc
     7861 ttgttgaaaa agctcgtcca gatcgcgttc aggatggggt agaacgagaa tatcgagaag
     7921 aagaccaggc cgggcgccgt gaaggcccag ccccagcggg aacggttccg ttcgagtacc
     7981 ccggaggtca cagccggttt catgggcgct ccgtatagag agagtatagg ttcgcggaca
     8041 cgatccgcgg gcccgggggc gaaaccgcag ccggccttcc cgggggacgc tccggccggc
     8101 gggggtcccc cgggaaggcg gggtgtccaa tacgtcggtt actcattgga cacccctggc
     8161 ggtttctaac gttctgttga gaacgttaga aaccgccacc ctaaaaccgg ctgcctagaa
     8221 agctatccga gcttcttgga cagccccggg cctgggactc actcgtcggc cagggccgcg
     8281 gtgacgtcct tccggagttt ctcgagggcc ttcgcaggct ccacaccgga aagcatgacc
     8341 gaagtgaagg cttccttcaa gagcgcctcg atcttggagc tggaggcgcc gtagtacacg
     8401 atgtgcccgg cggccatatc cttggcgaac acgtccgagt agggcatgtt cttgaaggtc
     8461 gcggactcca tcagggcctt ggtcggctgt acgatggcca ctttctccag gtactcggcg
     8521 ccgtggctca gcatgtagga caccagggcc caggacgcct gctggctcag cttgggcttc
     8581 tgggcgttca ccatgtagta gtggccgtag tagttgttcg gaaccttctt gaccgcgtcc
     8641 ttccactggg ggaaggggat gaccatccag tcggagctgt tgaagaaggc cggattcgca
     8701 ccccgcatgc gggcttcctg gtagagcccc gacagggaca tggcgatctc gttcttgtcg
     8761 ttgtcgaaga tgcggcgggc gtccgtatag gtcggggacc cgaggttctt cccgttcggg
     8821 ccgaaatccc ggaaataggt cagcaccttg atccacgcgg cgtcccccag gatggccttc
     8881 ttgccgtcct tggagatgag ctccccgccc agctgctcca ccatggggag cacggaggtc
     8941 aggtagtagc cgtagcggaa gtcgaagccc cggcgggtga tgatctgtcc gtcccgcttc
     9001 acgatcttct cggagaccgc gaccacgtcc tcccaggtct tcggccagtc cttgtccggg
     9061 tcgagtccgg cgtcccggaa gatcttcttg ttgaggtaga tgcaccagtt ggtcagctcc
     9121 agggggagcc cgtagacctt gccgccctcg gtcacggggt cgagcatgcc gggcaggtac
     9181 ttgccctgga ggtcccgggc gctcttgtag cccgcggcct tgaggtccac gggggccacc
     9241 cgtccgttca cgatgaactg gtactcgtcg aagatctcca ggttgaagat gtccgggccc
     9301 tggttggcgg agaacccggc caccacgatg tccttgatct tggacgaggg gtaggtgacg
     9361 tagttgacct tgacccccgg attggcggcc gaaaactccg cgatcaggcg cttttccagg
     9421 gccgtccggg cggggtcctc gtgggtccag atcgtcagct ccacggactt gcccttgagc
     9481 tccgggacag ccggggcctg ggccaggacg ggcagggaca gggcgatcac caggatcgcg
     9541 gtaaggattc gtttcatgcg tgcctccttg aaggatcggg acggccgtac ccggcggggc
     9601 ccggggatcg gggcgccgag ggtcttccct ggttccaggg tggggcggtt tcgggaaacc
     9661 cgcaatgcta cgattccggc atttggtcga aaaaaccggg ccgaccgggt cggattctcg
     9721 ggacacgttt tgccgagtcc cccgccggat tcgtatattc aaggagatct atagttcgac
     9781 acgaacgccg gaggctcgcc atgaccctta tgaaggaaat cgaggaacga cgcgcatacc
     9841 gagctctgtc cccggatccc ctgccggaag accaggtgga acgcctgctt acggccgccc
     9901 atcacgctcc ctcctgcttc aacaagcaga gctggcgctt cgtggcggtc agcggagacg
     9961 ccctggccgg gctccgggag accctctcgg aggggaacaa gtgggccacc cgggcacccc
    10021 tcatcgtggc ggccgcccag aagccctccc tggactgccg cctggacgag ggccgggatt
    10081 acgccccctt cgacctggga ctggcggtca tgagcctggt cctccaggcg acccacgagg
    10141 gcctggtagc ccatcccata gcgggatact cccccggcaa ggtgcggaag gccctgggca
    10201 tcccggagga ctacgtggta cataccctgg tcatcgtcgg cggaaaaggc tccgacgagc
    10261 tcctctcgga ctggcaaaag gagcgggaga cgggcccccg cctgcgggaa cccctggaga
    10321 aggtggcctt ccggaaccgc tggccggatc cggtttccgg ataagacaga caggatggac
    10381 aggatggaga gaggatgaac aggatcggca ttggtacttt cctgatctta tcctgttgat
    10441 caagaaaata tcctgttcat cctgtcagag tccttggaaa caacccccca tccggaaacc
    10501 gcccgttgcg gataccccga tgcggtggta tcctgcccct catggcgact gcgatgatca
    10561 tcggcatctg cgggggctcc ggggcgggca agaccacgat cgtgaggaaa atttccgagg
    10621 tcgtctcgga cttcgtcttc atcccccagg acaactacta caagtcggcc gagtacatct
    10681 ccaactccaa catcacggcc ttcaacttcg accaccccga ggccttcgac aacgaactgc
    10741 tctccgccca cctccgggag ctgaaggcga tgcggccgat cgagatgccg acctacgact
    10801 tcgtgcacca ccgccgggcg gagcagacga tcaaggtgac gccccggaag ctggttatct
    10861 tcgaggggat catggttttc accgataagg ttatacggga cctgatcgac ctgaagatct
    10921 tcgtggacac cccggacgac atccggttca tccggcggct cggccgggac atcaaggagc
    10981 gggggcggac ggtggattcc gtgatcgagc agtacctgaa cgtcgtgcgg ccggggcact
    11041 tcgagttcat cgagcctacc aagcgcttcg cggacatcat catccccgag ggcggggcga
    11101 acgagaacgc cctgacggtc ctgttgtcct tcatgcggaa catcatcgac ccgaggccgt
    11161 gaaggccgtt tcctaaggag ccctgaatgg tcctgcaaca agccctgcgc ctctcggcgt
    11221 ggacaagcac gttctccatc gcggccctgt tcgcggcgat cctggtcttc gccgcgccgg
    11281 ggcttccctt cctgcccacc accctgctcg gagccggatc cttcgtggtc ctcttcctga
    11341 tccaggacgc gctggtccgc gccctggact cggacctccg gaagcgccac ttcgaccggg
    11401 ccgaaacccg catcgtcatg gacttcgtgg agcgcctgcg ggtctccttc acggtggcgg
    11461 acctggtgga ggccgcccgg gactgcctgg agatgcgggc ggacgccagc gtcctgctca
    11521 tcaagagtgc cacctgggag acggtctacg ccagcccgtc caccctggcc aaggatccgg
    11581 agaccctggc gatcctgcac cggaacttcc gggaatggtc cgagggcttc gccttcctct
    11641 ccgacgagta cggcctgcat ccctcgaacg cgggctgccg cggctacctg agcgtctgca
    11701 agggatacca cctcttcgtc ttctcccgtt cggcgggact cgcggaactg gaggcgttcc
    11761 ggcttctcca cggagagctc cagctgtact tcgaccgcat catcacggtg gcccggctgt
    11821 tcaacatcgc gaacctctcc aaggaatggt ccctgatcgc ggaaacccag aagtccttcc
    11881 tgcccaagga gatgcccaag ctggccaagc tggagaccgc ctcctactac cgccccctgg
    11941 tcaacgtctc cggggattac tacgacgtca tacccgtcga ttaccgccgg accctcctgg
    12001 tcgtcggcga cgtgtccgga aagggcctgg ccgcggccct gatcatggga atcatcgtga
    12061 atacgatccg ggtggccccg gacaagacgg acctgccggc cctggtccgg aacgtggacg
    12121 aggcgatccg ggagatgcgg ttcgaggaca agtacacggt catcttcctg gggatcgtcg
    12181 acacggggga aaaggtgctc cgctacgtca acgcctccat ggcggacccc ctcgtgatct
    12241 cccagacggc cctgggcgcc aagcccaaga aactggggtc caccatgggt cttttaggcc
    12301 tcctgcccct ggaaggggag atccccgtgg aggagatacc cctgcgcacc gacgacgtca
    12361 tcctgatcgc ctccgacgga ctgctcgagc tggagaacag cgccggcgag cccctgagcg
    12421 agtccgccgc cttcgagcgg ctgctgcagc aatcctcccg ggagaccgcg gagagcatcg
    12481 tggagggcat cgcgggattc gcgtattcct tcgtggaggg gcgccagctc cgggacgaca
    12541 tcaccatcct ggccgcgaag gtgggcagac tatgggactg acgatcttcg ccttcctggc
    12601 tcctcccatc ctcctgggcc tctgggcccg cgcccggacg cggatccggg acaccgcctg
    12661 gagccggtac ttcctggcca tcgggatcgg cttctcggtc ttcacccttt ccctgggggt
    12721 cgcatacctg gccgcccagc cctacaccct cgaacagccc ctggctctct tcttcacccg
    12781 gctcgcggcc gcctcggccg cggcgggcta catcgtcctg ttccgggtct gcctgagctt
    12841 cccgtacgag acccggaacc gcatcgccga cctggcggcc gccgcggcgg tcctcggggt
    12901 cctggccctg accctgggca ccccgctcta cgtgagccat gtggagaggg tgaacctgga
    12961 cttcctccgc caggaaggcc ccctgtacgg ggcgatcagc accggggccg cggccctggg
    13021 gttcctgggc gccgccgtcc tcctgttccg aagcctggcc atgcgcagcc gcatcttcca
    13081 gcagcagggc ctgatcctgg ccctcgggat cgcggccagc atcgtcctgg gatacgtgac
    13141 ggcgatccgc ctgccccagg tcggccggat cgggtccctc tacctcgtct ccaccctcag
    13201 ccccgtcgtg gtcgccgtcg gattcttcta cgcggtgtcc acctcgcgga tcttcgcgtg
    13261 gaaggaggtg gcccgccgcc tggtctccta cctcatcctg gcgatcctcg cgggaacccc
    13321 cgtcggcgtc gcggtctccg tcttcctcat ggtgaaggcc tttccgctga tctccttcct
    13381 gggggccttt ttcagcttca tcctgggagc ccggatcgcc tccgccttcg tgaaccggtt
    13441 cctcgattcc atcgcgggac gcaagcgcta tcgcgaggac cttcagagct ccctggccca
    13501 cgtggacctc gcggggggcc gggacgcggt cctcaaggag gtgcacggga tactctccgg
    13561 ccccctggga ttctccgatt tcaccgtcct ggccgaggac gacgaaggcg agctccggcc
    13621 cgtgtattcg accaccggca cgagcctctc gatccggggg gattccgcgg ccctcggaac
    13681 cctgacggag cggggcatca ccgtgctcct gaagacggac gtcatcgcca accgctccta
    13741 tgcctcggtg aagaaggaac ttttggagat ctacgactcc ctgcgcgcgg aggcgatgat
    13801 cgtggtccgg gaagggcggg agaccctggg catcttcgcc ctcggcgccc ggctgtccgg
    13861 gggcgactac acgtcctacg attacgagac ctttagggcg gtgtacggca agctcttcgt
    13921 ggtcctctac tacgtcaaga acatcgcccg ggaatccatc ctgagcaccg tggaccggga
    13981 gatcgccttc tcgagccagg tcatcgagtc catccaggag aacgtcgacc ggatcgccca
    14041 ccccaaggcg gacgcccact tcatcgcccg gtccacccgc aagctgggcg gggacttcat
    14101 cgacttcgtc gggctcgggc gggaccggtt cttcttcgtc gtgggggacg tctccggcaa
    14161 gggcctgaac gcctccatgt ccatgctcat cctgaagacc atggtccgga acttcctgaa
    14221 ggaggagaag gacttccttc ggatcgccga gcgcgccaac tccttcatca aggagaacct
    14281 gccgaaggga accttcttcg ccggggtgtt cggctacttc gacttcgcga aggattccct
    14341 gtacttcatc aactgcggca tcccggtcat gcagatgtac tcgccggcct tcaacacggt
    14401 gatcgagatc cagggggacg gacgggtcct gggcttcgtg cgggatatcc ggccctacct
    14461 caagctccgc agggtgcccc tggtcaaggg ttccgccctg ctcatatcca cggacgggat
    14521 cctggaatcc gagagcctgc gcggggagcg atacggcaag gaacgcctcc aacggtcctt
    14581 caccgcgaac cgggaaggcc cggccaagga gcttgcggag gggctgctgc gggatctcct
    14641 ggccttcacg gacaaccgga tggaggacga cgtgaccatc tttacgttga aatacgacac
    14701 gaacgtcagg agcgaggcat gaaccagcta acagtcgagg aaatccggga aaccggacgg
    14761 accatcctgc aaatcgcggg accggtcaac agctacacgt tcacggattt ccaggagaag
    14821 atattcaagg ccgtccggcg ctcggacatc gtggtggaca tggagaaggt gacgaccctg
    14881 tcctccgcgg gcctgggcgt cctcatggcg gccatggagg atgcggaggg cgccggccgg
    14941 aagatctgga tcctgcggcc ctcggagatc gtccggctgg ccatcgagtc gaccggcttc
    15001 tcggaccggt tcccggtggc gcaatccatg aaagacctgg cctgacgaac cgcccgggcg
    15061 gcgcgaatcc ggtgtccgga atgaccgacc cctccaaatc ccccggtttc tcgacccgcc
    15121 tgcccgggga caggtcggcc atcccccgat tcgaggattg gctggacagg gccgcgatcc
    15181 cggatccgcg gctccgggat cgcgtccggc tggcggggct cgagatcctg gacaacctca
    15241 tccgccactc cggccccttg gaggggggct ccgtccggac ctcccttcgc ctccgggcgg
    15301 ggggtgcccg gctcgtgttc tccttccgat ccgtcaaatt ccggtccttc gcccggggcg
    15361 ggaagaatcc ccggacggga tacaacatcg aggacgggcg ctggcggggc ctgggactcg
    15421 tgatggtggc cttcctgacc ctgcacgtgc gctacgtatc ggacgcacgg agcgaccggg
    15481 tggaggcgga gttctaagga tctcgccgca tttcggaccc tgatcggacc tctttcccct
    15541 tgtgcgcgcc gtaccggtgt tgtatgatgg tggccccggt gctgcgcggc tggctcgcga
    15601 atacggccgg cggcacaaca ccaaggacgg aagccttgtg cgtccgtatt gtcctaaatg
    15661 tgaaggaaac cttcatgaaa tccaagatct ccatcctcct cgtcggcctc atggtcgtgc
    15721 tcgcgatcgc gggctgcgcc aagaaggaag ccccggcggc cgcgccgtcc ctgaaggtcg
    15781 gcatggtcac cgacgccggc accatcgacg acaagtcctt caaccaggga acctgggaag
    15841 gcgtcgttcg cgcggggacc gagttcaagc tcgacaccaa gtacctgaag ccctccggca
    15901 ccaccgaggc ggactacctc aaggaaatcg gcaacctcta cgacgcgggc tacaagttcg
    15961 tggtgacccc gggcttcaag ttcgagacca ccatcttcca ggcccagacc aagtacgcgg
    16021 acgccaagtt cgtcctgatc gacggcagcc cccacgccgg ggactggaag tccgtggtcg
    16081 gcccgaacac cgtgtccatc ttcttcgccg agcacgagtc ggccttcgtg gccgccgtcg
    16141 ccgcggccct gcagatcaag accgggtcct tcggcttcat cggcggcatg gagattcccc
    16201 cggtccagaa gttcaactgg ggcttccagc agggcgtgga ctacgccaat aagaacctgg
    16261 ggaccaagat caccatgaag gccgagaacg tgatctacca gggctccttc gacaacgtgg
    16321 ccgcgggaca gcagatcgcc gcctccatgt acgacaaggg cgtcagcgtg atcttcgccg
    16381 ccgccggcgg cgtgggcgtg ggcgccatca acgaggccaa ggcccgggcc accgcgggca
    16441 agaccgcctg gatcgtgggc gtggacgtcg accagtacgc cgagggcatc tactcgggcg
    16501 acaagtccat catcctgacc agcgccatga aggggatcga cgttgccgcc ttcgacatga
    16561 tcaaggccga gctgggcgga accttcccgg gcgggaagac cctgatcttc gacgccaaga
    16621 acaacggcgt cggcatcccc gccaagaacc ccaacctctc ccccgaggtc caggccaagg
    16681 tcgccgaagt cttcaagctc ctgcaggccg ggaccatcgc ggtcaaggcc gagcagggca
    16741 ccctcatcaa gtaagatccg gactccggaa tcccaaggct gtccaaaaag ctcagttagc
    16801 tttttggacg gctggttccc gtgcagggtg tccaataagt gaccgactta ttggacaccc
    16861 tatttttctt gtcgattccg ccgggggcgg attatactgc gtatccgaat tgaaaaaatc
    16921 ttggccaggg agaccctccc ctccttggcc ttctccaagg aggggacatg gaatacatcg
    16981 tagagatgct cggtatcacc aaggatttcc ccgggatacg cgccaacgac gacatcaccg
    17041 tacggctccg gaagggcgag atccacgccc tcctgggcga gaacggggcg ggcaagtcca
    17101 ccctgatgag catcctcttc ggcctgtacc ggccggactc cgggatcatc aaggtacggg
    17161 gcaaggaagt ctccatccac aaccccaacg tggccaacgc cctggggatc ggcatggtcc
    17221 accagcactt caagctggtg cacaacttca ccgtgaccga gaacatcatc ctgggcatgg
    17281 aacccaccgc gggtcccatg ctgcagaccc gggacgcggc caagcggatc aaggccctgt
    17341 ccgagcagta cggcctgaac gtggacccgg acgccaaaat cgaggacatc tccgtgggga
    17401 tgcagcagcg tgtggagatc atcaagatgc tctaccgcga cgccgaggtc ctcatcttcg
    17461 acgagcccac cgcggtgctc accccccagg aaatcgatga cctcatgcag atcatgcgga
    17521 acttgctcaa ggaggggaaa tccatcctcc tcatcaccca caagctcaag gagatcaagg
    17581 ccatcgcgga ccgctgcacg gtcatccggc gcggcaaggt cgtcgacacc gtggacgtcg
    17641 cggacgtggc ggaatcccgc atggcggaga tgatggtggg ccgcaccgtg aacttcaagg
    17701 tggacaagcg ggaacaggaa cggggagagg cggtcctctc gatccggaac ctgaacgtcc
    17761 tgtccagccg taagacccag gccctgaaga acttctccct ggacgtgcac cggggggaga
    17821 tcctgggact ggcgggtgtg gacgggaacg gccagaccga gcttgtggag gccctcaccg
    17881 gactgcgcaa ggtggagtcc ggctccatcc tcttcaaggg ccgggacatc acccacgacg
    17941 acatccattc ccgggtgatg gccgggatgg cccacatccc ggaggaccgg cagaaacggg
    18001 gcctcgtgct ggactactcc atggaggaca acttcatcct ggagatctac ggccttccgc
    18061 cctactcccg gaagggattc ctgaaccgca aggccatccg ggaccacgcg gaaaagctga
    18121 tccaggactt cgacgtgcgg tccggccagg gggccctctc cgtgggccgc accctgtcgg
    18181 gaggcaacca gcagaaggcc atcgtgggcc gggagatcga ccatgacccc gacctcctcc
    18241 tggccgtcca gcccacccgg ggcctggacg tgggctccat cgagtacatc cacaagcggc
    18301 tcgtggaaca gcgcaacaac ggcaaggcgg tgctcctcgt atccctggag ctggacgaga
    18361 tcctggatct ctcggaccgg atcgcggtca tcagccacgg ggagctcacg ggagtcaagc
    18421 tgcccaagga aaccaacgag agcgagatcg gcctcatgat ggcgggcatc gcgggcgcgg
    18481 gagggatgga atgaaccgct acaagagtct gtt
//
LOCUS       DAOTEI010000057        18318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2035_length_18318_cov_5.307178, whole genome shotgun sequence.
ACCESSION   DAOTEI010000057 DAOTEI010000000
VERSION     DAOTEI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 18318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18318
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2035_length_18318_cov_5.307178"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="dinB"
                     /locus_tag="P5313_07395"
     CDS             complement(<1..768)
                     /gene="dinB"
                     /locus_tag="P5313_07395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRY80223.1"
                     /translation="MQFHVDMDAFYASVEQLDRPELAGMPVIVGAAPGHRGVVSTCSY
                     EARAFGVHSAMPISEAVRLCPGGIFLPVRMERYLELSRTVMDILGEFSPDLRQISVDE
                     AFLDMTGTEGLWGAPREAATRLKEEVRNRTGLTVSVGAASNRYIAKIASGFRKPDGLT
                     IVPPGGEEEFMSGLPIGKLWGAGEKTQERFRELGIRSVPQLRAIGKAGLSALFGNAGG
                     AFLYAAACGQDPGIFRTEPGSRSMSTERTFGRDIADPE"
     gene            complement(778..3000)
                     /locus_tag="P5313_07400"
     CDS             complement(778..3000)
                     /locus_tag="P5313_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80224.1"
                     /translation="MRIPAGSGRSLVLSLLLALAVARVPAEPSGVRDEVLAAVRGMGT
                     RGENTPGERALFDFLEAEIRRKGLRTALVPIADHVEGHSFSGILEVRIPGTGPGTLAL
                     GIPVSDAQPWGDGAEGISAALELMDLFIRSPPPLELRFAFLGADEDLTGSRAYAAYCA
                     DEPSLAAVRVEVPDARSGSAEILIGGTGSLSPYWLLNAATRAARGLGIREVVQANRSM
                     IQRLGLVDEPTSLDPWFQRGIPAVTLRAGAAENPDSGPGSAGIVQILENLVRSLESGI
                     PDRWDRQYVLFEAFGLRIAVRETLYVASILALYAGLGLVFVFDSLRNRDILVEELSRI
                     PRGAAALAMVSAALLACVLASGALQSLVLWASGSGEYWKVRPVAFSVLRLVQILTLFT
                     ALASLGARLGLLPRKAEFFRGSAVVILGADVLLVSTVRLSLSLLFLWAFVIALAGRRA
                     SRALRSSWPAALSLPLMLLPLGLLLADLVRDPELEVFSKFLLPGAPGTAWIVFLALPF
                     LMVFVGLGEGLFGKGFYKARTSSISAVLFLAVSLAAGAWLVSDARGYSGTTEISIREY
                     SDETGLAGRVRIESPRLIPAFRLSGGDLQAEFPGGRSGEISVPLWSGSRIGMSLDRTA
                     FLDRVQLSLSIKTEGAPRGIRISIPSMDSGSVYDTSFPFRPTEDGRGIVIFIGARPPN
                     PVVVSLTVSREFSALAEIAAVFPTPASPLILEGPVRLRDYAQESRLYAYLRSDGGPVR
                     "
     gene            3105..4271
                     /locus_tag="P5313_07405"
     CDS             3105..4271
                     /locus_tag="P5313_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80225.1"
                     /translation="MSTQERKSILDLPVKVILTEAGTTFFINNNKTLKKFRLADNVEE
                     YGVFLDRFAPMSLQRMLLVDYVSKVEISKSEFMTSRQEIMDLSKLIVYSLLYRQYDSY
                     IFSRILSSDLIKKWNRLNPANIIDEKTRINDTFLQNVLKEKEPDIREIKQQILNPLYA
                     MINHNAALLPDEKNIHLLLSEKFLTTLRPFVWFIIAKFKGSDGYEQMITDIRTSLAEY
                     MEKSKIAEYVSLMIMELAANSENSNLKREAKEIFKGAVDMNAVLFDPNIRRQVIESLE
                     RKRELVYLSWKIGSKSTSIGTQGRLQITVFNKESEYQRMKEAVEQKKNADLKKKSLQD
                     FYKEIPETEANTELGLYYLSYLSEACEKVNVKFESHVNQIPGSDLTLVSMSLNL"
     gene            4365..7214
                     /gene="ppdK"
                     /locus_tag="P5313_07410"
     CDS             4365..7214
                     /gene="ppdK"
                     /locus_tag="P5313_07410"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014487137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRY80226.1"
                     /translation="MAKTKYVYFFGSGEAEGDGKQKELLGGKGAGLAEMTKIGLPVPG
                     GFTITTEVCDLYYKNGKKYPEGLKDEVEKHLKRLEKVAGKKLGDPKDPLLVSVRSGAA
                     ISMPGMMETILNLGLTDASVEGLAAKTGNRRFALDAYRRFIMMYGSTAMGIEREEFDH
                     AFDAVKESLTRKRLGIKAGVKVGDTDVNEEELAVLIKNFKALYKKHIKSDFPQDPIVQ
                     LWGAVGAVFGSWMADKAVTYRKVEKIQGVRGTAVNIVQMVFGNKGDTSGTGVCFTRDP
                     NTGENVFYGDYLINAQGEDVVAGIRTPIKLSDFASADPKAYKQLLDVRKTLEKHYKDM
                     QDLEFTVEEGKLYMLQCRTGKRSPAAAFKIAVDLVNEKLITKEEAISRIKTSDIEGVF
                     YPGIDKSQTKMLKEAFLVEGIAAVPGAATGKIAFSAAKAEAQAANGEKVILVRKETSP
                     EDVGGMHAAQGILTATGGKTSHAAVVARGWGKSCIVGCEKINIDYEKKTMSAGPAVLK
                     EGDYITLDGTSGAVYKGELKLVKAEPPEAYNTIMGWVDKIRTIKVRTNADTPYDAENA
                     RKLGAEGIGLCRTEHMFFDSEARILAIREMIIADDVEARKKALAKLLPFQTEDFRGIF
                     KAMDGYPVTVRLVDPPLHEFVPHDEAGQKALAKAVGVPYETVRRRVEQLHEANPMLGH
                     RGCRLTITYPEILDMQVTAIIKAACDMAKKGVKVLPEIMIPLVIDAKELKILEVRTRQ
                     VADKIIKDAGVKLPYLVGTMIEVPRAALLADQVGGVAEFFSFGTNDLTQMTLGMSRDD
                     AGRFLPDYVDEKKAGIFKTDPFQSLDQEGVGMLVKMAIERGRQSRPNLKVGVCGEHGG
                     DLVSVKFFHRAGMNYVSASPFRVPIARLAAAQAVIEDAAKAKPAAKKAPAKAAAAKKP
                     SAPKAAVAKQPAKKAVPAKPVTKPAVKKAAPKGKK"
     gene            7503..8048
                     /locus_tag="P5313_07415"
     CDS             7503..8048
                     /locus_tag="P5313_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRY80227.1"
                     /translation="MLTQNTAWLNAERAVQNLSNAGALSHEGILAMSREILAEIIRPA
                     GYFNAKARTLRELAGFFRTLSPDGFRYSAPTRDSLLSVRGIGPETADSILVYGFGVPT
                     FVVDAYTRRIAGRLGFEHTASSYEDFKSHIEMNLPRKWTVYAEFHAILVEHAKVRCRK
                     SPRCAGCPLGDLCKYRMYEKT"
     gene            complement(8100..8552)
                     /gene="dtd"
                     /locus_tag="P5313_07420"
     CDS             complement(8100..8552)
                     /gene="dtd"
                     /locus_tag="P5313_07420"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRY80228.1"
                     /translation="MRAVVQRVEKASVSVEGRITGSIEAGLLVYVGIGKGDSIKDADW
                     LAGKIADMRIFPDEEYKMNRSVRDVGGSILAVSQFTLYADVRKGRRPSYDKAASPDSA
                     RSLYEHFLEQLRGTGIEIRTGEYQAVMDVSYVNKGPVTILLDTEQGSR"
     gene            complement(8549..9646)
                     /locus_tag="P5313_07425"
     CDS             complement(8549..9646)
                     /locus_tag="P5313_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80229.1"
                     /translation="MVSDPLRKAQKDFLKGYYSRVLSSLEPLTLSYRDSHLFYFLMGT
                     SCLATGDVGGAATYLRRAEQLNFRHSPTLAALAAVHVRRGETDKAIQLYLDILSREPG
                     NVFAKRALLFIKSNSEPERLAALVKSGGIAALYPLPPTARRLAARYLPAFLIAALAAF
                     LSLALIPRAATLLPKAPSGRAGLESFLLTEAERENPVSAAGGFEYVLTEKEAVNSFER
                     AKKFFASWNDEAALVEINRILLSNAAPSIKSKAETLKGFVREPDFTNIREKYSYSDVA
                     RSPRLFDGVAVLWKGQAGNIRDSGSALQLDLLVGYQDKKNLEGIVAVSFPFTLRVTAG
                     VPLEILGRVRPSKDGFRLEGIAVHELRDGSP"
     gene            complement(9652..10698)
                     /locus_tag="P5313_07430"
     CDS             complement(9652..10698)
                     /locus_tag="P5313_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80230.1"
                     /translation="MKHRRMLLLAAAVLLSAAAAWTLDLRDGRILVSLDERTARLSVH
                     YLADAARNRYVSLLSEQDARTSYPTLAWNGKFYRLGESADFRISVRREGANAVVEYRS
                     SFALVRQTVLFLRSRGAPDADGVMMEFAIENLSGNPAAAGLRILLDTYQGERGKRHFE
                     IDGTGIVGAELSLSGSSIPARLLTPGDSGATLQIQLSGEGLTRPDRLILSNWKRINDA
                     EWGFEASPGRSFTLLPFSIEDSAAALYFEPSELRSGGTRIIRTALGNRTETGYSTSPI
                     PGGSPPEAAASPQAAMSVKPDPFVGARTDIAALREILARIDALLAAGGTPDPAQVEEI
                     RRLLGNIRSRPREY"
     gene            complement(10695..11621)
                     /locus_tag="P5313_07435"
     CDS             complement(10695..11621)
                     /locus_tag="P5313_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY80231.1"
                     /translation="MRRFRTAPALLVALILPFAAYPQTVPDGRIERGIRLFGEEKYSE
                     ALELFNRVLADPAAQTDRPDAVYWSGLSYMALGDAVNARRTLDTFLKEYPRHPSVPDA
                     LYQRARVAYTSGEYEESILLFSTFLEKYPDHSFASSALFWTADGLFSLGRLAESETLF
                     RTLLADYPKSVKFEAASYKLALIRYKYRENELLTLLKWSHEESLRVIEEFQRREKAYE
                     QALAVYQRRLADTGASLAVPAPAAAPADSDQRIAALNARIEELTKALAAQAPSPSADK
                     SAELLALKAETLELLSLYIDWLAGNVEKGARP"
     gene            complement(11641..12372)
                     /gene="rpe"
                     /locus_tag="P5313_07440"
     CDS             complement(11641..12372)
                     /gene="rpe"
                     /locus_tag="P5313_07440"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRY80232.1"
                     /translation="MDLPALPIVAPSILSADFSDIRGALRLCEDAGAPWLHLDVMDGR
                     FVPNLTFGAKMVSDIRKRTRLFLDAHLMTVEPERLAPAFLDAGADAVTFHLEACVHAH
                     RLASEIRSRGAAAGVSIVPSTPVSALEPLLPFLDLVLIMTVNPGFGGQKLIPECLDKV
                     SELRGIRERRGLTFRLSVDGGINESTVPAAARAGAEVLVMGSAFFEAPDPGLLLRSIR
                     SAYTLEASASARPAEAATAGPPGRR"
     gene            complement(12529..13659)
                     /locus_tag="P5313_07445"
     CDS             complement(12529..13659)
                     /locus_tag="P5313_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRY80233.1"
                     /translation="MLDSILEFIQSTGFVAMKWTDLIMITVSCVLLYLGIVKKFEPLL
                     LVPIAFGMLLANIPLGGIMDPAKDGQPGGLLYYLYYGIKFGIYPPLIFMGVGAMTDFG
                     PLIANPKSLLLGAAAQLGIFVAFLLARALGFDLQGAASIGIIGGADGPTAIFLTSKLK
                     PELLPAIAIAAYSYMALVPIIQPPIMNALTTKKERMVVMDQLREVSKAEKIIFPIMVT
                     ILCGLLLPAAVPLLGALMLGNLFRECGVTERLNNTAQNALINILTIFLGTAVGATASA
                     DKFLTVQTLSIVGLGLLAFAFGTAGGVLLGKLMYMLSGGKINPLIGSAGVSAVPMAAR
                     VSQVVGQKANPSNFLLMHAMGPNVAGVIGSAIAAGVLLSMLG"
     gene            complement(13685..14059)
                     /locus_tag="P5313_07450"
     CDS             complement(13685..14059)
                     /locus_tag="P5313_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRY80234.1"
                     /translation="MKSYKIYVNGQAFDVSVEEVGGSAPAAPAPAAASAPAPRPAPAA
                     PAASPAPAAGASSVKAPMPGTILGVKVAPGQAVKKGQVLLILEAMKMENEICAGGDGT
                     VAAVRVQAGQSVNTGDPLVDMA"
     gene            complement(14082..14453)
                     /locus_tag="P5313_07455"
     CDS             complement(14082..14453)
                     /locus_tag="P5313_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRY80235.1"
                     /translation="MSNIGLYEWTVVLIGMGTVFITLIGLWWILEGFSVVAGRLARKK
                     AEAVRSADLPPSAPAQAPVRASAGVTPEVVAAITAAVAAATGRGPSEIRIARISPSSP
                     GLAGLNTPVWGYANRLAAGRR"
     gene            complement(14463..16004)
                     /locus_tag="P5313_07460"
     CDS             complement(14463..16004)
                     /locus_tag="P5313_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRY80236.1"
                     /translation="MEEKIRELVKRRDKITAGGGPKRVAAQHEKGKMTARERIRMLLD
                     EGSFVELDAFVEHRCDELGMADVEAPGEGVVIGYGTVAGRLVYVFAQDFTVIGGSLGE
                     MHAAKICKAMDMAMKVGCPVIGINDSGGARIQEGVDALSGYGDIFYRNTVASGVVPQI
                     SVIMGPCAGGAVYSPALTDFVLMTDKSSNMFITGPQVIKAVTGEDVSAEDLGGSHVHC
                     EKSGVAHFRFADESGTIAGIKRLLSFLPQNNLEDPPLADPVAPAGDPSALADIIPDSP
                     NKPYDVRRIIEGIFDGGDFFEVQEDYAKNIVVGFARLAGRPVGIVANQPAVMAGVLDI
                     NASDKGARFIRFCDAFNIPLVTFADTAGYLPGVSQEHGGVIRHGAKLLYAYSEATVPL
                     LTVILRKAYGGAYIAMCSRHLGADQVFAWPSAEIAVMGPEGAANIIFKKEIEEASDPA
                     AARKEKIEEYKRSFANPYKAAVRGYVDDVLDPAETRSRLVAALAMLGGKRETRPARKH
                     GNFPA"
     gene            complement(16092..16493)
                     /gene="mce"
                     /locus_tag="P5313_07465"
     CDS             complement(16092..16493)
                     /gene="mce"
                     /locus_tag="P5313_07465"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRY80237.1"
                     /translation="MTEKLDHIGIAVENLEQSLAFYRDVLGLELHGTETVEEQKVRVA
                     FFPVGDTEIELLESTDPEGPIAKFIAAKGQGVQHLAFRVKDIEKTLADLKARGVRLID
                     EKPRYGAGGAKIAFLHPKATNGVLVELCQRD"
     gene            complement(16499..17572)
                     /gene="meaB"
                     /locus_tag="P5313_07470"
     CDS             complement(16499..17572)
                     /gene="meaB"
                     /locus_tag="P5313_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRY80238.1"
                     /translation="MSDVPETVRRIRSGDPRTLARTISRIDDGDPSVAQVLRALHTHT
                     GRAHVIGVTGPPGSGKSTLVDKFVGEFRRRGLSVGVLAVDPSSPFTGGAILGDRIRMQ
                     SHALDEGVYIRSLATRGHLGGLSGAVMEAVRVLDAAGFDRILIETVGVGQSEVDIVRL
                     ADTVVLVSVPGLGDDIQVLKAGIMEIGDVFVVNKSDRDGADRVVREIRSMLETALVNG
                     VTPRALSGAGVADRGGHHIASAARESANAHGYGDAHGLPPVLKTTAETGEGVMPVVDA
                     VEAHRAALEESGLLRERRLRNAAYELRDALGKRLLARLDSPDALARLDALARDVVERR
                     RDVYDAVDALHEALSLEEKHPRR"
     gene            complement(17584..17979)
                     /locus_tag="P5313_07475"
     CDS             complement(17584..17979)
                     /locus_tag="P5313_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRY80239.1"
                     /translation="MDRKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVVYTGLRQTP
                     EQIVQAAVQEDADVIALSILSGAHNHLFPRVMELLAQKGVNDVLVIGGGVIPEDDIPF
                     LKKAGIAEIFGPGTPTKATIDFIRANLKR"
     gene            complement(17992..>18318)
                     /locus_tag="P5313_07480"
     CDS             complement(17992..>18318)
                     /locus_tag="P5313_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRY80240.1"
                     /translation="YTYQMDVESGERIVVGLNKFQIKEAAPEGLLKVDPAVGKMQEKK
                     LADLRGRRDNAAVRTALEGLATAAQGSENLMPRILEAVRQYATLGEICDVLRGVFGEY
                     QQKVVL"
BASE COUNT         3416 a         5945 c         5920 g         3037 t
ORIGIN      
        1 ttccgggtcg gcgatgtccc ggccgaaggt ccgctcggtg ctcatggagc gggagccggg
       61 ctctgtacgg aaaattcccg ggtcctgtcc gcaggcggcc gcgtacagga aggcacctcc
      121 ggcgtttccg aagagggccg agagacccgc cttcccgatg gcccggagct gggggacgct
      181 ccggattccc agttcccgga acctctcctg ggtcttttct ccggcgcccc agagcttccc
      241 gatcgggagg ccggacatga actcctcctc ccctccgggc ggaacgatgg tgaggccgtc
      301 gggcttgcgg aacccggacg ctatcttggc gatgtagcgg ttggaagccg cacccacgga
      361 gacggtcagg ccggtccggt tcctcacctc ctccttgagc cgggtcgcgg cttcgcgggg
      421 cgccccccac aacccctcgg tcccggtcat gtccaggaag gcctcgtcca cggagatctg
      481 ccgcaggtcc ggggagaact cccccaggat atccatgacc gtccgggaca gctcgaggta
      541 gcgttccatg cgcacgggga ggaagatacc ccccgggcag agccggacgg cctccgagat
      601 cggcatggcg gagtgtaccc cgaaggcccg tgcctcgtag gagcaggtgg ataccacgcc
      661 ccggtgtccc ggggccgcac ccacgatgac gggcatgccg gcaagctccg ggcggtccag
      721 ctgttccacc gaggcgtaga aggcgtccat gtccacgtgg aactgcatgg gacgttccta
      781 ccgaaccggt ccgccgtcgc ttcgcaaata ggcgtagagc cggctctcct gcgcatagtc
      841 ccggagccgc accggtcctt ccaggatcaa gggcgaggcg ggtgtcggga aaacggcggc
      901 aatttccgcg agggccgaaa attcccggga aaccgtcagg gagacgacga cgggattcgg
      961 cggccgggct ccgatgaaga tcacgatgcc cctgccgtcc tcggtggggc ggaagggaaa
     1021 ggacgtgtcg tacacggagc cggaatccat ggaggggatc gagatccgta tcccccgggg
     1081 agccccctcg gtctttatgg acaggctcag ctgaactctg tccaggaacg ccgtcctgtc
     1141 cagggacatc ccgatccggg agcccgacca caggggaacc gagatttcgc ccgagcgccc
     1201 cccggggaac tcggcttgga ggtccccccc cgaaagacgg aaggccggta tcagccgcgg
     1261 gctctcgatg cggaccctgc ctgcaaggcc ggtctcgtcc gagtattccc gaatggatat
     1321 ctccgtcgtt cccgagtatc cccgggcgtc cgagaccagc catgcccctg cagccaggga
     1381 aacagcaagg aagaggaccg cggaaatcga ggaagtcctg gctttataga accccttccc
     1441 gaacaggcct tccccgaggc cgacgaatac catgaggaac gggagggcga ggaatacgat
     1501 ccacgccgtg cccggagctc cgggcagaag gaatttcgag aagacctcga gctcgggatc
     1561 ccggacaaga tccgccagga gaagtccaag gggaaggagc atgagcggaa gggaaagggc
     1621 cgcgggccag gaacttcgca aagctcggct cgccctcctt cccgccaggg cgatgacgaa
     1681 ggcccacagg aacaggaggg aaagcgatag ccgcacagtg gagaccagga gaacgtccgc
     1741 ccccaggatc acgacagcgc ttccccgaaa gaattccgcc ttgcgcggca ggagaccgag
     1801 acgggcaccg agggaggcca gggcggtgaa gagggtcagg atctggacca accggaggac
     1861 ggagaaggcg accggtcgga ccttccaata ttccccggag cccgaggccc ataggacgag
     1921 gctctgcagg gcgccggaag ccagcacgca ggccagaagg gccgcggaca ccatcgccaa
     1981 ggccgcggct ccccggggga tccggctcag ttcctcgacc aggatatccc ggttccgcag
     2041 ggagtcgaat acgaagacca gtccgaggcc ggcgtacagg gcgaggatgc tggcgacata
     2101 cagggtctcc cgtacggcga tcctcagccc gaaagcctcg aagagcacgt actggcgatc
     2161 ccaacgatcc gggattcccg attccaggga ccggaccagg ttctccagga tttgaacgat
     2221 ccccgccgat cccggtccgg aatccggatt ctccgcggcc cccgcccgca gggtgacggc
     2281 ggggatccct cgctggaacc acggatccag ggaggtcggc tcgtccacga gacccaggcg
     2341 ctggatcatg gaacggttcg cctggacgac ttctcggatt cccaagcccc gggccgcccg
     2401 ggtcgcggcg ttcagtagcc agtacggcga gagggagccg gttcctccga tgaggatctc
     2461 cgcggagccg gaccgggcgt ccggaacctc gacccggaca gcggccagcg agggttcgtc
     2521 tgcgcagtag gcggcgtagg cccgggatcc cgtcaagtcc tcgtcggcgc ccaggaacgc
     2581 gaaccgaagc tccaggggcg gcggggaacg gatgaaaagg tccatcaact ccagggccgc
     2641 ggatattccc tccgccccgt caccccaggg ctgggcgtcc gagaccggga ttccgagggc
     2701 cagggtcccc ggacccgtac ccggaatccg cacttcgagt atcccggaga aagaatgtcc
     2761 ctccacgtgg tccgcgatcg ggaccaaggc cgtcctcagg cccttccttc gaatctccgc
     2821 ctcgagaaaa tcgaacaggg cgcgctctcc cggggtgttc tccccgcggg ttcccatccc
     2881 ccggacggcg gccagtacct cgtcccggac gcccgacggt tccgccggaa ctctcgcgac
     2941 ggccagggcc aggagaaggg atagaaccaa ggatcgaccg gatccggcgg gaatgcgcat
     3001 acctcggtcg agcatagggg gggcatcctt cttcgtcaat agttgtacgg gccctggatt
     3061 ctaagctata ctggacggga gtccaaacgg ggagaatggc gcagatgtcc acccaggaac
     3121 gcaagagcat cctcgacctg cccgtaaaag tcattctcac ggaagccggg accaccttct
     3181 tcatcaacaa caacaagact ctcaaaaaat tccgcctcgc ggacaacgtg gaggaatacg
     3241 gggtcttcct ggaccgcttc gcccccatga gcctccagcg catgctcctg gtggactacg
     3301 tgtccaaggt cgagatctcc aagagcgagt tcatgaccag ccggcaggag atcatggacc
     3361 tgtccaagct catcgtttat tccctgctct accgccagta cgactcctac atcttctccc
     3421 gcatcctttc cagcgacttg atcaagaagt ggaaccgcct gaatccggcc aacatcatcg
     3481 acgagaagac ccgaatcaac gacaccttcc tgcagaacgt actcaaggag aaggaaccgg
     3541 atattcggga gatcaagcag cagatcctga accccctgta cgccatgatc aaccacaacg
     3601 ccgccctcct gccggacgag aagaacatac atctcctgct ctcggagaaa ttcctgacca
     3661 ccctgcgccc cttcgtgtgg ttcatcatcg ccaagttcaa gggatccgac ggctacgagc
     3721 agatgatcac ggacatccgg acctccctgg cggagtacat ggagaagtcg aagatcgccg
     3781 agtacgtctc cctgatgatc atggagctgg ccgccaactc cgagaactcg aacctcaaac
     3841 gggaggccaa ggagatcttc aagggcgccg tggacatgaa cgcggtcctc ttcgatccca
     3901 acatacgccg ccaggtgatc gagtccttgg aacggaagag ggagctggtc tacctctcct
     3961 ggaaaatcgg ctccaagagc acctccatcg ggacccaggg gcggctccag atcacggtgt
     4021 tcaacaagga atccgagtac cagcgtatga aggaagcggt cgaacagaag aagaacgcgg
     4081 acctcaagaa gaagagtctc caggatttct acaaggagat tcccgagacg gaggcgaaca
     4141 cggagctggg cctgtactac ctatcctacc tctccgaggc ctgcgagaag gtcaacgtga
     4201 agttcgagtc ccacgtgaac cagattcccg ggtcggatct taccctggta agcatgagtc
     4261 tcaacctcta gccccgcccg gcttccttga cactcccttc ctctttctat agctttacac
     4321 agtccagact tccatagacg cgcatataac ggaggattca ttcgatggcc aagaccaagt
     4381 acgtctactt cttcggttcc ggagaggcgg agggcgacgg caagcagaag gagctcctgg
     4441 gcggcaaggg tgccggcctg gcggagatga ccaagatcgg cctgccggtt cccggtggct
     4501 tcacgatcac cacggaggtc tgcgacctct actacaagaa cgggaagaag tatcccgagg
     4561 ggctcaagga cgaggtggag aagcacctca agcgccttga gaaggtcgcg ggcaagaagc
     4621 tcggcgatcc caaggacccc ctccttgtct ccgtccgatc cggcgctgcc atctcgatgc
     4681 ccggcatgat ggagaccatc ctgaacctgg ggctcacgga cgcttccgtg gagggcttgg
     4741 ccgccaaaac cggcaaccgc cgcttcgccc tggacgccta ccgccgtttc atcatgatgt
     4801 acggttccac cgccatgggg atagagcggg aggaattcga ccatgccttc gacgcggtaa
     4861 aggaaagtct gacccgcaag cgcctgggca tcaaggcggg cgtcaaggtc ggggacacgg
     4921 acgtcaacga ggaggagctg gccgtcctca tcaagaactt caaggccctc tacaagaagc
     4981 acatcaagag cgatttcccc caggacccga tcgtccagct ctggggggcc gtgggagccg
     5041 tcttcggatc ctggatggcc gacaaggccg tcacctaccg caaggtggag aagatccagg
     5101 gagtccgagg caccgcggtc aacatcgtcc agatggtctt cggcaacaag ggcgacacct
     5161 ccggcacggg ggtctgcttc acccgggacc cgaacaccgg ggagaacgtc ttctacggcg
     5221 actacctgat caacgcccag ggcgaggacg tggtggccgg catccgcacc cccatcaagc
     5281 tctcggactt cgcctccgcg gaccccaagg cctacaagca gctcctggac gtccgcaaga
     5341 ccctggagaa gcactacaag gacatgcagg acctcgagtt caccgtcgag gaaggcaagc
     5401 tctacatgct ccagtgccgg accgggaagc gttcccccgc cgcggccttc aagatcgccg
     5461 tagacctggt caacgagaag ctgatcacca aggaagaggc catcagccgc atcaagacct
     5521 cggacatcga gggcgtcttc tatcccggca tcgacaagtc ccagaccaag atgctcaagg
     5581 aagccttcct cgtggaaggc atcgccgcgg tacccggagc ggcgaccgga aagatcgcct
     5641 tctccgcggc caaggccgag gcccaggccg cgaacggtga gaaggtgatc ctagtccgca
     5701 aggaaaccag tcccgaggac gtggggggca tgcacgccgc ccagggcatc ctgaccgcca
     5761 cgggcggcaa gaccagccac gccgcggtcg tggcccgggg ctggggcaag tcctgcatcg
     5821 tcggctgcga aaagatcaac atcgactacg agaagaagac catgtccgcg ggccccgcgg
     5881 tcctcaagga aggggactac atcaccctgg acggcacctc cggagccgtg tacaagggcg
     5941 aactcaagct ggtcaaggcc gaacctcccg aggcctacaa caccatcatg ggctgggtgg
     6001 acaagatccg gaccatcaag gtgcgcacca acgcggacac cccctacgac gccgagaacg
     6061 cccgcaagct gggtgccgag ggcatcggac tctgccgcac cgagcacatg ttcttcgaca
     6121 gcgaggcccg catcctggcc attcgggaga tgatcatcgc cgacgatgtc gaggcccgca
     6181 agaaggccct ggccaagctc ctccccttcc agaccgagga cttccggggg atcttcaagg
     6241 ccatggacgg ataccccgtc acggtccgcc tggtcgaccc gcccctccac gagttcgtgc
     6301 cccacgacga ggccggccag aaggccctgg ccaaggccgt gggagttccc tacgagaccg
     6361 tccggcgccg ggtggagcag ctccacgagg ccaaccccat gctcgggcac cggggctgtc
     6421 gcctgaccat cacctacccc gagatcctgg acatgcaggt caccgccatc atcaaggccg
     6481 cctgcgacat ggccaagaag ggcgtcaagg tcctgccgga gatcatgatc cccctggtca
     6541 tcgacgccaa ggagctgaaa atcctggagg tccggacccg gcaggtcgcc gacaagatca
     6601 tcaaggacgc gggggtcaag cttccctacc tggtgggcac catgatcgaa gttccccgtg
     6661 ccgctcttct ggctgaccag gtcggaggcg tcgcggagtt cttctccttc ggcaccaacg
     6721 acctcaccca gatgaccctg ggcatgagcc gggacgacgc gggccgcttc cttccggatt
     6781 acgtggacga gaagaaggcc ggaatcttca agaccgatcc cttccagagc ctggaccagg
     6841 agggcgtggg catgctggtg aagatggcca tcgagcgcgg ccgacagtcc cgcccgaacc
     6901 tcaaggtggg agtctgcggc gagcacggcg gcgacctggt ttcggtgaag ttcttccacc
     6961 gggccgggat gaactacgtc tccgccagcc ccttccgggt tcccatagcc cggctggccg
     7021 cggcccaggc tgtcatcgag gacgccgcca aggccaagcc cgcggccaag aaagcccccg
     7081 ccaaggcggc ggccgcgaag aagcctagcg cgcccaaggc cgcggtcgcg aagcagccgg
     7141 ccaagaaggc cgtgcccgcc aagccggtta ccaagcccgc ggtgaagaag gccgccccga
     7201 agggcaagaa ataaatatgg acgtgggcgg agggacgcag gctcccccca cgtttcccgt
     7261 tgaaggcccc ctcgctcgcg aggcgggcct tcattttttg ccggtcccgg gttcaaggtt
     7321 aaagggcgtt ccgaatgcga tggatggacc tatatcaaac attgctggaa acctacggcc
     7381 cccggggatt gtggccccgt atctcccggg ccggtacgga cggccgggat gaccggggct
     7441 accttccggg aggcgggccc ccggaagacc tttccggggc gttcgaaatc gccgcagggg
     7501 ccgtgctgac ccagaacacc gcgtggctca acgccgagcg ggcggtccag aacctgtcga
     7561 atgcaggcgc ccttagccac gaagggatcc tggcgatgtc gcgggagatc ctcgccgaga
     7621 tcatccgtcc cgccgggtac ttcaacgcta aggcccggac gctgcgcgaa ctagcgggat
     7681 tcttccggac cctctccccg gacggcttcc ggtactccgc gccgacccgg gattccctgc
     7741 tctccgtccg ggggataggc cccgagaccg cggattcgat cctcgtatac ggcttcggcg
     7801 tacccacctt cgtggtcgac gcctacaccc gcaggatagc cggacgtctg gggttcgagc
     7861 atacggcttc ttcgtatgag gacttcaaat cgcatattga gatgaaccta cctaggaagt
     7921 ggaccgtgta cgcggaattc catgcaatac tcgtggaaca tgccaaggtc cggtgcagga
     7981 aaagcccgag atgtgcggga tgtcctcttg gagatttgtg taagtataga atgtatgaaa
     8041 aaacataatg ctactgaagt attaaagaaa cgacggtgtt ggaagtttac aaccggttac
     8101 tatcttgaac cttgctccgt atccaacagg atggtgaccg gtcccttgtt cacgtaggac
     8161 acgtccatca ccgcctggta ctcgccggtc cgaatttcga tgcccgtacc tctaagttgt
     8221 tccaggaaat gctcatacag gcttcgggcg gaatccggag aggcggcctt gtcgtagctc
     8281 ggcctccgtc ccttgcgtac gtccgcatac agggtgaatt gcgataccgc gaggatcgaa
     8341 ccgccgacat cccggaccga acggttcatc ttgtattcct cgtccgggaa aatcctcatg
     8401 tccgcgatct ttccggcaag ccaatcggca tccttgatcg aatcgccctt tccgatgccc
     8461 acgtagacga gaagcccggc ctcgatgctt cccgtgatcc gtccctccac ggacacggaa
     8521 gccttctcga ccctctgtac gaccgccctc acggactgcc gtcccggagt tcgtggaccg
     8581 cgatgccctc cagccgaaag ccgtccttgg aaggccggac ccgcccgaga atctccaagg
     8641 ggacccccgc ggtgacccga agggtgaagg gaaaggatac ggcgacgata ccctccaggt
     8701 ttttcttgtc ctggtatccg accaggagat ctagctggag tgccgaaccc gaatcccgta
     8761 tgtttccggc ctggcctttc caaagcaccg cgactccgtc gaacagtctt ggagacctcg
     8821 ccacgtccga gtacgaatac ttctcccgga tattcgtgaa atccggctcc cggacgaagc
     8881 ccttcagggt ttccgccttg ctcttaatgg acggagccgc gttggagagc aggatccggt
     8941 ttatctcgac cagggcggcc tcgtcgttcc aggatgcgaa aaatttcttg gctcgctcga
     9001 aggagttgac ggcttccttc tcggtcaaca cgtattcgaa tccgcccgcc gcggacacgg
     9061 gattctctcg ttctgcctcg gtaagcagga agctctccag tccggcacgg ccggagggag
     9121 ccttgggcag gagcgtggcc gcccgaggga tcagagccag ggacaggaag gccgcgagtg
     9181 ctgcgatgag gaacgccggg agatacctcg ccgccaggcg ccgcgccgtc ggcgggaggg
     9241 gatacagcgc ggcgattcct ccggatttga ccagggcggc cagacgctcc ggttccgagt
     9301 tggacttgat gaagagcagg gccctcttcg cgaagacgtt tcccggttcc cggctcagga
     9361 tgtccaggta gagctggatc gccttgtcgg tctctccccg gcggacatgg acggccgcga
     9421 gggccgccaa ggtaggtgaa tgtcggaaat tgagctgttc cgctcgccgg agataggtgg
     9481 ccgctccccc gacgtccccg gtggcgagac agctggtgcc catcaggaag tagaaaagat
     9541 gcgaatccct gtacgaaagg gtcagaggtt ccagggagga aagaacccgg gaatagtatc
     9601 ccttcaggaa atccttctgc gcctttctca ggggatcgga aaccatgggc tctagtactc
     9661 ccggggtctg gaccggatgt ttcccagaag cctgcggatc tcctcgacct gggcgggatc
     9721 cggagttcct ccggcggcca ggagggcgtc gatccgggcc aggatctccc taagggccgc
     9781 gatatccgtg cgggcgccca cgaagggatc cggtttcacg gacatggccg cctgggggct
     9841 cgcggcggct tccgggggcg atccgcccgg gatgggggac gtggaatatc cggtttccgt
     9901 cctgttgccc aaggcggtcc ggatgatccg ggttccgccg ctccgcaatt ccgaaggttc
     9961 gaagtagagg gccgcggcgg aatcctcgat ggagaacgga aggagcgtga agctccttcc
    10021 gggggaggcc tcgaatcccc actcggcgtc gttgatccgc ttccagttgg acaggatcag
    10081 ccgatccggc cgggtcaatc cctccccgga aagctggatc tgaagggtgg ctccggaatc
    10141 accgggggtg agcagtcgtg ccgggatgga ggagcccgac agggagagct cggctcccac
    10201 gatgcccgtt ccgtcgatct cgaaatgccg cttgccgcgc tcgccctgat acgtgtccag
    10261 caggatcctg agccccgcgg ccgccgggtt cccggacagg ttctcgatcg cgaactccat
    10321 catgacgccg tccgcgtcgg gagctccccg ggaacggagg aagagtacgg tctgccgtac
    10381 cagggcgaag gaagaacggt attcgacgac cgcgttcgcc ccttccctcc gtacgctgat
    10441 ccggaaatcg gcgctttctc cgagccgata gaacttcccg ttccatgcga gagtcggata
    10501 gctggttcgg gcatcctgtt cggacaggag ggagacgtaa cggttccggg ccgcgtcggc
    10561 caaatagtgg acggagagcc gcgcggtacg ttcgtccagg gataccagga tccgtccgtc
    10621 ccgaaggtcg agcgtccagg ccgccgcggc ggacaggagg accgccgcgg ccagaagcaa
    10681 cattcttcgg tgtttcatgg ccgggctcct ttctccacgt tgcccgccag ccagtcgatg
    10741 tacagcgaaa gcaactcgag ggtttccgcc ttcaaggcaa gaagttcggc gctcttgtcc
    10801 gccgagggtg acggggcctg ggccgccagg gccttggtca gttcctcgat gcgggcgttc
    10861 agggccgcga tcctctggtc ggaatccgca ggggcggcag cgggggccgg caccgcgagg
    10921 gaggccccgg tatcggccag tcttcgttgg tataccgcca aggcctgttc ataggccttc
    10981 tccctgcgct ggaattcctc gattacccgg agggattcct cgtggctcca cttgagcagg
    11041 gtgaggagtt cgttctcccg gtacttgtag cggatcaagg cgagcttgta ggacgccgcc
    11101 tcgaacttca cgcttttagg gtagtccgcc agcaaggtac ggaacaaggt ctccgactcg
    11161 gcgaggcggc ccagggaaaa gagtccgtcc gcggtccaga agagagccga ggacgcgaag
    11221 gaatggtcgg gatacttttc caggaaggtg gaaaacagca ggatggattc ctcgtactcc
    11281 cctgaggtgt aagctacccg ggcgcgctgg tacagggcat ccggcacgga aggatggcgg
    11341 ggatattcct tcaggaaggt gtccagggtc cggcgcgcgt tcacggcgtc tcccagggcc
    11401 atgtagctca atcccgacca gtataccgcg tcgggccggt ccgtctgggc cgccgggtcc
    11461 gcgagtaccc ggttgaagag ctccaaggcc tcggagtatt tttcctcccc gaaaagccgt
    11521 ataccgcgtt cgatccgtcc atccggaacg gtctgagggt aggccgcgaa gggaaggatc
    11581 aaggccacca ggagggcggg tgctgttcgg aatcgtcgca tcaggattcc tccgattgga
    11641 ttatcggcgt cccgggggtc ccgctgtagc cgcctcggcg ggccgggcgg aggcggaggc
    11701 ctccaaggtg taggccgaac ggatcgagcg aagaagcagg ccgggatcgg gagcttcgaa
    11761 aaaggcgctt cccatcacga ggacctcggc ccccgcgcgg gcggcggccg ggaccgtgga
    11821 ttcgttgatg cctccatcta cggagagtcg gaacgtgagg ccccggcgtt cccggattcc
    11881 gcggagctcc gagaccttgt cgaggcattc gggaatcagc ttttggcccc cgaagccggg
    11941 attcacggtc ataataagga cgaggtccag gaacggaaga aggggctcca gggccgagac
    12001 cggcgtggag ggcacgatgg acacccccgc ggcggcgccc ctgctccgga tttccgaggc
    12061 gaggcggtgg gcgtgtacac aggcttccag gtggaacgtg acggcgtcgg ctccggcgtc
    12121 caggaaggcc ggagccagac gctcgggttc cacggtcatg aggtgggcgt ccaggaaaag
    12181 ccgggtgcgt tttcggatgt ccgagaccat cttggccccg aaggtcaggt tgggtacgaa
    12241 tcgaccgtcc atgacgtcca ggtgcagcca gggcgcaccg gcatcctcgc agagcctgag
    12301 ggctccgcgg atgtccgaga aatccgccga cagtatggac ggggctacga tcggcaaggc
    12361 tgggagatcc atgcgctcat actagcatag gggtcggtgc ggggcaagga cgaccgggag
    12421 gcgggatgaa aaacgccgcg ccccggaggg cgcggcttcc ggtaaagcga cgaagcccga
    12481 agggcttcgt cgcctcctgg ggtcggctgt cgccgacccc aggagcagct agccgagcat
    12541 ggacagaagc acccctgccg cgatcgcgga gccgatgacc cccgccacgt tcggtcccat
    12601 ggcgtgcatg agcaggaaat tcgacggatt cgccttctgg cctacgacct ggctgacccg
    12661 ggcggccatg ggaaccgcgg ataccccggc gcttccgatg aggggattga tcttcccgcc
    12721 cgacagcatg tacatcagtt ttccgagcag gaccccgccc gcggtgccga aggcgaaggc
    12781 caggaggccc aggccgacga tggagagggt ctggacggtg aggaacttgt ccgcgctggc
    12841 cgtggctccc accgccgtgc ccaggaagat ggtcaggatg ttgatcagtg cgttctgggc
    12901 cgtattgttg agacgctccg tgactccgca ctcccggaag aggttgccca gcatcagggc
    12961 gcccagcagg ggcacggccg cgggcagcag cagtccgcac aggatagtga ccatgatcgg
    13021 gaagatgatc ttctcggctt tgctgacctc gcggagctgg tccatcacga ccatgcgctc
    13081 cttcttggtg gtcagggcgt tcatgatcgg cggctggatg atgggcacca gggccatgta
    13141 ggagtacgcc gcgatggcga tggccggaag gagttccggc ttcagcttgg aagttaagaa
    13201 gatggccgtc gggccgtccg cccccccgat gatgccgatg gaggccgctc cctgaaggtc
    13261 gaagccgagc gcccgtgcca agaggaaggc cacgaagatc ccgagctggg ccgccgcccc
    13321 caggaggagg ctcttggggt tcgctatgag gggtccgaag tccgtcatgg cacctactcc
    13381 catgaagatg aggggaggat agattccgaa cttgatccca tagtagaggt aatacaggag
    13441 ccctccgggc tggccgtcct tggcgggatc catgatgccc cccaggggga tgttcgccag
    13501 aagcatcccg aaggcgatgg gcacgaggag gaggggctcg aacttcttga cgatccccag
    13561 gtaaaggagc acgcaggata cggtgatcat gatgaggtcg gtccacttca tggccacgaa
    13621 tccggtgctc tggatgaatt ccaggatact gtccaacatc tgcgtatttc cttatgcggc
    13681 aggcctaggc catgtccacc agcggatccc ccgtgttgac ggattgtccg gcctggacgc
    13741 ggacggccgc caccgtgccg tcgcctccgg cgcagatctc gttctccatc ttcatggcct
    13801 ccaagatcag caggacctgg cccttcttga cggcctggcc cggggccacc ttgacgccca
    13861 ggatggtgcc gggcatggga gccttgacgg aggacgcgcc cgcggcggga gccggggatg
    13921 ccgcgggggc ggccggggcg gggcggggcg ccggcgcgct cgcggcggcg ggcgccgggg
    13981 cggccggggc ggaccctccc acttcctcga cactcacgtc aaaggcctgt ccgttcacgt
    14041 agatcttata gctcttcata tccaactcct tacgaattcg atcagcggcg gcccgcggcc
    14101 agccggttcg cgtaacccca gaccggggtg ttgagcccgg ccaggccggg agaggacggc
    14161 gagatgcggg cgatccggat ttcggaaggc cctcggcccg tcgccgcggc caccgccgcg
    14221 gtaatcgccg cgacgacctc gggggtcact ccggcggaag cccggaccgg agcctgggcg
    14281 ggtgcggacg ggggaaggtc ggcggaacgt accgcctcgg cctttttccg cgccagccgg
    14341 ccggccacga ccgagaagcc ctcgaggatc caccagagtc cgatcagggt gatgaaaacc
    14401 gtgcccatgc cgatgaggac cacggtccat tcgtacaatc cgatgttgct catgctccgc
    14461 ccctaggccg ggaagttccc gtgcttgcgg gccgggcggg tttcccgctt gccccccagc
    14521 atggccaggg ccgcgaccag gcggctccgg gtttccgcgg gatcgaggac atcgtccacg
    14581 tagccccgca ccgcggcctt gtagggattg gcgaaggagc gcttgtactc ctcgatcttt
    14641 tccttccggg cggcggcggg atcggaggct tcctcgatct ccttcttgaa gatgatgttg
    14701 gccgcgccct cgggacccat gaccgcgatt tccgcggacg gccaggcgaa gacctggtcc
    14761 gcgccgaggt ggcgggagca catggcgatg taggcgccgc cgtaggcctt gcggaggatc
    14821 accgtaagga ggggcacggt cgcctcgctg taggcgtaga ggagcttggc cccgtggcgg
    14881 atcacgcccc cgtgctcctg gctcaccccg ggcaggtagc cggcggtgtc cgcgaaggtc
    14941 accaggggga tgttgaaggc gtcgcagaaa cggatgaacc gggcaccctt gtcggaggcg
    15001 ttgatatcca ggaccccggc catgaccgcg ggctggttgg ccacgatccc gacggggcgg
    15061 ccggccaggc gggcgaagcc gaccacgatg ttcttggcgt agtcctcctg gacctcgaag
    15121 aagtcccctc cgtcgaagat cccctcgatg atccggcgaa cgtcgtaggg cttgttcggg
    15181 gaatcgggga tgatgtccgc cagggcggac ggatcccccg cgggggcgac ggggtccgcc
    15241 aggggcggat cctccaggtt gttctggggc aggaaggaaa ggaggcgctt gatccccgcg
    15301 atcgtcccgg actcgtccgc gaaccggaaa tgggccaccc cgctcttctc gcagtggacg
    15361 tgggacccgc ccaaatcctc ggcggagacg tcctccccgg tcacggcctt gatgacctgg
    15421 ggaccggtga tgaacatgtt ggagctcttg tccgtcatga gcacgaagtc cgtgagagcc
    15481 ggggaataca cggcaccgcc ggcgcaggga cccatgatga cggagatctg gggaacgacc
    15541 ccggaggcaa cggtgttccg gtagaagatg tccccgtacc cggacagggc gtccacgccc
    15601 tcctggatgc gggccccgcc ggaatcgttg atgccgatga ccggacagcc caccttcatg
    15661 gccatgtcca tggccttgca gatcttggcc gcgtgcatct cccccaggga cccgccgatg
    15721 acggtgaagt cctgggcgaa gacgtagacc agccgtcccg ccacggttcc gtagccgatc
    15781 accacgccct ccccgggcgc ctccacgtcg gccatgccga gttcgtcgca gcggtgctcc
    15841 acgaaggcgt ccagctcgac gaaggacccc tcgtccagga gcatccggat gcgctcccgg
    15901 gccgtcatct ttcccttctc gtgctgggcg gccacccgct tgggtccgcc cccggccgtg
    15961 atcttgtccc ggcgcttcac gagttcgcgg atcttctctt ccatacccct accttctccc
    16021 gtcggctccg ccgacgctgg ttctattatt cgggacgccc gaaggcgtcc gtgattcctg
    16081 ccgtacggcc ttcagtccct ctgacagagc tccaccagca ccccgttggt ggccttcggg
    16141 tggagaaagg cgatcttcgc acccccggcc ccgtaccggg gcttttcgtc gatcagccgg
    16201 acccctcggg ccttgaggtc ggccagggtc ttttcgatat ccttcacccg gaaggccagg
    16261 tgctggaccc cctgcccctt ggcggcgatg aatttggcga tgggcccctc gggatccgtg
    16321 gactccagga gctcgatctc cgtatccccc acggggaaga aggcgaccct gactttctgc
    16381 tcctccacgg tctcggtccc gtgcagctcc agacccagga cgtcccggta gaacgccagg
    16441 gactgctcca ggttctctac ggcgatgccg atatgatcca gtttttccgt cattccgcct
    16501 acctcctagg gtgtttttct tcaaggctca gggcttcgtg gagggcgtcc accgcgtcgt
    16561 acacgtcccg gcggcgctcc accacgtccc gggccaacgc gtccagccgg gccagggcgt
    16621 ccggggagtc caggcgagcg aggagccgct tgcccagggc gtcccgcagc tcgtacgccg
    16681 cgttccggag ccgccgctcc cggaggagcc cggactcctc gagggccgcc cggtgggctt
    16741 ccaccgcgtc gaccacgggc atcacgccct cccccgtctc cgccgttgtc tttagcaccg
    16801 ggggcaggcc gtgggcgtcc ccgtacccgt gtgcgttcgc ggattcgcgg gcggcgctgg
    16861 ctatgtggtg cccgccccgg tccgcgaccc cggcgccgga tagggcccgg ggggtgaccc
    16921 cgttgaccag ggcggtctcc aacatggaac gtatctcccg caccacccgg tccgccccgt
    16981 cccggtccga cttgttcacc acgaatacgt ccccgatctc catgatcccg gccttgagca
    17041 cctggatgtc gtcccccaga cccgggaccg agaccagtac gaccgtgtcc gccaggcgga
    17101 cgatgtccac ctcgctctgg ccgactccca cggtctcgat caggatccgg tcgaagcccg
    17161 cggcgtccag gacgcggacg gcctccatga cggccccgga aagcccgccc aggtggcccc
    17221 gggtggccag gctgcggatg tagaccccct cgtccagggc gtgggactgc atccggatgc
    17281 ggtcccccag gatcgcaccg cccgtgaagg ggctcgacgg atccaccgcc agcaccccca
    17341 cggaaaggcc gcgccggcgg aactccccca cgaacttgtc caccagggtg ctcttgccgg
    17401 atcccggagg tccggtgacc ccgatgacgt gggcgcgtcc cgtgtgggtg tggagtgccc
    17461 ggaggacctg ggcgacggag ggatccccgt cgtcgatccg ggagatcgtg cgggccaggg
    17521 tccggggatc cccggaccgg atcctccgga cggtttccgg aacgtcggac atcgggaatc
    17581 ggcctagcgc ttcaggttcg cgcgaatgaa gtcgatggtc gccttggtcg gggtcccggg
    17641 accgaagatc tccgcgatcc cggccttctt caggaaagga atgtcgtctt cggggatgac
    17701 cccgccgccg atcaccagta cgtcgttcac gcccttctgg gccagcagct ccatgaccct
    17761 ggggaagagg tggttgtggg ctcccgagag gatcgagagg gcgatgacgt ccgcgtcctc
    17821 ctggacggcg gcctggacga tctgttccgg ggtctgtcgt agacccgtgt acaccacttc
    17881 catgcccgcg tcccggaggg cccgggcgat caccttggcg ccccggtcat ggccgtccag
    17941 cccgggcttg gcgactaaca cacgtatttt cctgtccatt ccgtaccccc gttaaaggac
    18001 gaccttctgc tggtattccc cgaatactcc gcgaagcacg tcgcagatct ccccgagggt
    18061 ggcgtactga cgcaccgcct ccaggatgcg gggcatgagg ttttccgaac cctgggccgc
    18121 ggtcgccagc ccttccagag ccgtccgcac ggccgcgttg tcccgtcttc ccctcaggtc
    18181 cgccagcttc ttctcctgca tcttgccgac cgcggggtcc accttcagga ggccctccgg
    18241 agcggcttcc ttgatctgga acttgttcag ccccacgacg atccgctccc cggactccac
    18301 gtccatctgg taggtgta
//
LOCUS       DAOTEI010000058        18307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2039_length_18307_cov_6.086621, whole genome shotgun sequence.
ACCESSION   DAOTEI010000058 DAOTEI010000000
VERSION     DAOTEI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 18307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18307
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_18307_cov_6.086621"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="P5313_07485"
     CDS             complement(<1..447)
                     /locus_tag="P5313_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY80241.1"
                     /translation="MGAYVLKRLGQSLITVSIVVLTVFLLLRLMPKNGYFTREDYVNM
                     DRDARTAYLRNLGVLDDPFTQFGRFLSGLARGDLGRSITVYPRSPVLDVIGEKAPYSL
                     WFGLAALALSMALGLGLGILMARFKDRFPDGAGTVYVTIVRAIPSLI"
     gene            complement(656..2545)
                     /locus_tag="P5313_07490"
     CDS             complement(656..2545)
                     /locus_tag="P5313_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY80242.1"
                     /translation="MKSRAMIIAVLMALAATAGAWAAGVDRYYAVYASEGSSLRYFYT
                     PTTTDQRVAANTQDGLVEQDRFGRFVPSLAQSWTVSPDGLTWTFKLRQGLMYVDSSGQ
                     KTRWEITADDFVEGLRYVSDPKNGIKNFSRDVRNLIAGLNGYYLDLSDVDSGKKKDMT
                     REDALANFSKVGISAPDKYTLVYKLSKPTPFFLSYLVMELFLPVEKEFLDSVGLDFGV
                     SKEKLVFSGAYYISDWQRSKRIVLKANPHYWDAGKISVKTITLQYVPDPTTQVAMFQR
                     GELSRADLGADQVKALAKSKWGQYIYSDPPSADRFSVTYWFVQNFTSSNPEFKAFVNN
                     LNFRKALYYGIDRVKLNELDDPYKPGQIIRNTVVPEFQIFDEKGVDYTDYTGVKEIKA
                     AGNYYNKAKARDYFKKAVAELTDGKGTIKGVSPATVDYKPIAEVKVDGKLPLQMVYVS
                     LPDAVETKRALLLKAMLEEAFGKENIEVTLGQCIDDVFGEVVEPRRFDLLHDNFRFGY
                     ADPAAQLSRLVTNGGVNDGQYSDPEFDKLVQDAMSKTVLSERYAIFAKAEALYLDRCY
                     VLPWAMGGTAYQITKVVPFTYPRGGFGITRFKFKGMVVEKDPITAKRYEQLKAAFYKE
                     MAAAK"
     gene            complement(2650..3843)
                     /locus_tag="P5313_07495"
     CDS             complement(2650..3843)
                     /locus_tag="P5313_07495"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRY80243.1"
                     /translation="MVKGDLFIAGGNLRTRARDVHAAFVKAAGGPGGRFAVLGAASKV
                     PRSGFEFFRNSLTSLGVDPSRIELLPVAAAVPGWEDGARDPAVAERAARADGIWMLGG
                     DQNALTDTLLEPDGSDTPLLAVMRRANEGGAPMGGSSAGAAVMSDPMIAGGTSFGALA
                     LPRAVEAADVEISRALYVRRGLGFFPVGIVDQHFDARARLGRLLEACRVEDGGRRPGF
                     GIAEDTALVWRAAEGRAEILGAGGVYVVDLREAIRGLPGGRVEIRHARLHYLTEGDSF
                     RPADWFVEFRGKEPLDPGDPSFDVSRPQASGILSEYGTLADFAFRMLLDNHPEGLLRG
                     PDRGFRYVRSFLVEEGDDDLPLAWEVRLGRKEGLTRGWTGDQFGFEHVFVDILPAEIE
                     IKLFR"
     gene            complement(3943..5976)
                     /locus_tag="P5313_07500"
     CDS             complement(3943..5976)
                     /locus_tag="P5313_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY80244.1"
                     /translation="MKVAVVAGSESTARVLVRQLAEYVEGRAEIVPYWVDEGLSEPPE
                     GDLVVLSSDLVRKDLEASGLLPVGTEPLVARRVVDCDALERVVALPPGLPVLFVNDRP
                     ETARDCVDSLRDLGLDGVDWLPWNPEDPPPPRSYRIAVVAGEPDLVPPGMDEVIDIGV
                     RILDFGSLAEILKRLGMAETDIGGYSRRYLAKIVNLARRLARSTEEARRLSGHLGSVI
                     DSLRHGILVYDEEGRVSVCNEELRDLLHLRPGSGPGAALGSLIRNRELLGFLDCRCGE
                     EEGTFKLPEGTLVVRRFDLGEGGHTVAVFRGEGPEASRLGREYRRRGHVAKWTLEDIA
                     GESEAVRRAKRIATRLAATDLSILIHGESGTGKELFASAIHAASARSAGPFLAVDLGA
                     LSDDLVESELFGYEEGAFTGAKKGGKTGLFELADGGTLFLDEIGNVSPKVQTRLLRVL
                     QEKEVMRVGGADLKRVDVRVIAATNEDLLDKARKGQFREDLYFRLKMGWLRIPPLRER
                     RQDIPRLVRRILEQEGAGDVAVSPEVLAALESRDWPGNVRELRNALTYMLAVREDREI
                     SAADLPEHEYFAPREAEPYRPALSGDAGPAAPLGFEDRFVLGAVAELEAEGKAAGRDT
                     IAALARARGLGLGTGAARSRLERLAALGLLVMGRGRRGSRLTAEGRRAVRSVH"
     gene            complement(5973..7169)
                     /gene="iadA"
                     /locus_tag="P5313_07505"
     CDS             complement(5973..7169)
                     /gene="iadA"
                     /locus_tag="P5313_07505"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HRY80245.1"
                     /translation="MLLLVQNADLYAPERIGPRDILIAAGRIVAIADRIDPAGLPAPC
                     EVLDARGRFVVPGFVDSHVHVTGGGGEGGYRTRTPELVLSGALRAGVTTVVGVLGTDG
                     VARSMEALVAKLYGLREEGISAYGYTGSYRVPLTTVTGDPVRDIMMIEPILGIGELAI
                     SDHRSSKPTDAELARIASEARLGGMLSGKAGVVNVHLGDAPAGFEPLERIVAPGDLPR
                     TQFLPTHCNRNPIVLEQSVAWTLAGGYADYTTSTVPRFLEEGESSAAESFALCLSRKV
                     PADRITWSSDGQGSLPSFDAAGKLSGLLVGTCASLLSALREAVKVRGIPLETALLPIT
                     ANPAAVLKLVRKGRIAPGLDGDLTVLDRDLKPVSVVARGRVMIRDGQELVSGTFENID
                     PLEAAP"
     gene            7472..8410
                     /locus_tag="P5313_07510"
     CDS             7472..8410
                     /locus_tag="P5313_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRY80246.1"
                     /translation="MQHVLLAFYILFFATGFMGGAALFVLGLRIRSRLIRPLLAFQAL
                     FLVGLGLVALYAYLYGLWGAVPPTLALVLGTVLTGMNAAIYAVAILLVRRISPPASRR
                     KAYPAAAEILAGLVIAKSLASLVLALAGASRPGARALLDTEAWSLAGYILTGLAMAAF
                     GVTARGPLPPGEAPALRPLFKAYGTCALAFAPLGLVEYAVETAGLPGLPFLSLDHLFY
                     LSWNIVSMSAAVRLLKPGDAGVPALDSVPAERIEALGLSGREAQMAVLIARGLANKEI
                     AAELGISPATVRTHIYNLYQKAGARSRVELINKLRA"
     gene            complement(8462..8821)
                     /locus_tag="P5313_07515"
     CDS             complement(8462..8821)
                     /locus_tag="P5313_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80247.1"
                     /translation="MERKQPKNPALYGALLGLSGLFTLAAILTLVPNPGARWENVLGY
                     RSLCTFAPIATVICAFLAGATCVIRARIAGPRAGEKRSLVAPVVVAAALAAVVALTLP
                     AYLSVKADVGTGASVAP"
     gene            complement(8852..9520)
                     /locus_tag="P5313_07520"
     CDS             complement(8852..9520)
                     /locus_tag="P5313_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRY80248.1"
                     /translation="MPRPVSFSREHIGTAALDLVRREGWDALTARGLAAALGTSVAPI
                     YGAFESMESLQAWVLGEASRLLRASGTRPRTDSGFLNSGIGLVLFARDEPRLFLALYR
                     AGMDGELERHRREVRSRMKEDAFLASLGEGELDRIFDQLWTYTMGMVMAMLFGHAKDR
                     SEEGAASRLRSAGSLLMYGVLSGLDEDGSPEARERWTDLFERRGWTIPPRAGDFRNGP
                     APGR"
     gene            complement(9663..10844)
                     /locus_tag="P5313_07525"
     CDS             complement(9663..10844)
                     /locus_tag="P5313_07525"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HRY80249.1"
                     /translation="MYDFDTVPDRVPTGCVKWSARRELCGEEDAIPLWVADMDFPVAP
                     EIQEAIRARAEHPIYGYPVRQDGFYESLTGWTRRRYGWEIRREWICYSPGIVPAVNLA
                     VLAFTRPGDKVVIQSPVYYPFGAAVLNNGRRLLDNPLRLEGDRYVMDLPDLERKIDSR
                     TKLLILCSPHNPVGRVWTREELSALAELCARKNLVVVSDEIHADIVMPGRRHTCFAAI
                     SEDAASRSLTCLAVSKTFNLAGLCTANVVIPDRRLRDGFSTAAGNLGLGTSNVFGIAA
                     QEAAYTKGEPWLEELIRYVRGNYLRLKTVLEGGIPGIRVLPLEGTYLAWADCRGLGMS
                     DPELKDFFLHKAKVWLDEGIKFGTGGSGFQRFNLACPRATLDAALDRILSAYEARRAA
                     D"
     gene            complement(10914..12089)
                     /locus_tag="P5313_07530"
     CDS             complement(10914..12089)
                     /locus_tag="P5313_07530"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRY80250.1"
                     /translation="MDENVGSHFGTWWAVAVWAALFGAFLLFVPFYRKADRKPAGVFL
                     GFVVAFALEMFGAPLSLYFVLWAFGKTLPEGVLWGHTLSGTFGMTGHYVYLACVLAGA
                     VLVVGGWARIHREYWSREEGQGRLVAEGFYRYVRHPQYTGFLLVSLGVLFEWATIPLL
                     ILWPILASMYVRLARKEETEMEERFGDEWRAYKARTGMFLPRFGKAPVLVLAALILAG
                     AAGPAWAGEGLTWKSYAEIGGGATAAGGEIRSLASLESGIFLGRVELGSYLHLIPLKF
                     GSPDLIQQGALVYGGSLGFRLGPGAAGIVPYVRLGLGGIAKDEADGSGAFDGQGVEKS
                     FCGILTLGAEASLGGRWSARLWGSCRLAPGYEDFEGRGLSGFDLGASIRATWWTTLR"
     gene            complement(12332..15199)
                     /locus_tag="P5313_07535"
     CDS             complement(12332..15199)
                     /locus_tag="P5313_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY80251.1"
                     /translation="MPYRFRVFAAAMVLAAFAPAARAQDRPADPEMELSQLEAERMLS
                     SILRSAPGGIGVVVDRVIVQVNDYIVQLTGYSREELLGKSARIFYPADEDYEFVGREK
                     YRQIAERGTGTVETRWRTKEGEILEVILSSTPLDTADLSEGVVFTVTDITEKNRTART
                     LRDRTRTFLAGSLALIVLQLGLILALIRSSALRHKAAEALRLSQERFDLAMLAVNDGI
                     WDWDIPSNTVYFDPRYYKIAGYEPGDFPHRFDEFEKRVHPDDLERVLAHSQAHIDGRV
                     IQFDIEFRFLRKDGTWMWIRGRGKVVARDGAGKVLRMIGTHTDVTDKKAAEEDLRKSR
                     ATLDLVLNTIPQSVFWKDRDGRYLGCNQAFARDVGLEDPARILGKTDFDLPWTAEDAE
                     AYRADDREVVESGRPKRHIVEPLQQADGARLWIDTTKVPLVDEGRVFGVLGIYEDVTE
                     RKLMEQRLWESTRMLNDVIDTIPVRVFWKDRAGRYLGCNRSFAQDAGKDRPEDVIGLD
                     DFSLLWKDQAEAYRADDRSVVEQDQAKINYEEPMSSDGGRKWLRTSKIPLKDPEGRIY
                     GILGVYEDITERRRVDEELKALNETLEQRILDRTRELDRMNSSLVKANEELQATLETL
                     HEAQDSLVQSEKLAALGQLIAGIAHELNTPLGAIVSSNQSLIHLIGNRLEEASRTVAG
                     LSPEVLTWFNAALKLSLGSRPLEEEASGRFRRRKGLAARLADCGRPIPDRLLDALLDL
                     RLDGDPSIPALVRDHPDCPEAVYALDTLSSIKNITDVIAVAADKCSNVVSALLYYVRK
                     EEDEDASSRVEIPKELDSLLTLYTNKTKYGVRIEKDYGCPGLVKGNRNQLNQVWMNLI
                     QNALQAMDFRGTLGLSVREGDSRIVVEVRDTGPGVPAHLREKIFEPFFTTKKSGEGTG
                     LGLDICRRIVEKHGGRIELESEPGRTLFRVLLPAWKDDSTA"
     gene            complement(15208..16254)
                     /locus_tag="P5313_07540"
     CDS             complement(15208..16254)
                     /locus_tag="P5313_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY80252.1"
                     /translation="MAERYVLCVDDEAIITESLKRELRHELPHLRVEAAYSGETALSL
                     LAEISQDGGEPAVLVTDERMPGMKGHILLREARKAFPRLYGILLTGYSDIDAIAQAVN
                     EAGLFRYIHKPWERRDLVMSVRRAAELYDREAELELLRRKVEELNTALIAALENQARD
                     WDPDSFCHVQRVACYAALLGRKLGLAEAEVRKLYTYAPLHDIGKAGIPHEILDKPGRL
                     TPEEFEVVKRHVEIGTNLLKSIDIDEFAKDLILYHHERWDGKGYLAEMVGARIPLSAR
                     ITAVADSLDAMLTKRPYKDALPFDEVAREIEADSGKRFDPAVVEAFRTEKDSFRQISA
                     GDASRHCGKFRDPA"
     gene            complement(16436..16849)
                     /locus_tag="P5313_07545"
     CDS             complement(16436..16849)
                     /locus_tag="P5313_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80253.1"
                     /translation="MKRFLNVVLLCLAAGSAFAQSDSEIRTLIGKAELIGELKVNFKA
                     DFDVLPVRLKEGALRRLVFLAEGNDVEIERIVVTYGDGRKDEIPLRHRFAEGTRSRAV
                     DLEGGRRLIRTITFHYRTEGRLKDGRAVVKVYGIK"
     gene            complement(16905..18152)
                     /locus_tag="P5313_07550"
     CDS             complement(16905..18152)
                     /locus_tag="P5313_07550"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRY80254.1"
                     /translation="MPNFVAPPRRPGLPAAAALLTAWILWSLPGGGLSAQEPRTGKPR
                     LSVLYFTDTRGSADLAWLSKGLADLLAGDLGASGAFTLVEREELERVLREQELALSGL
                     VDPAGAPKVGRLLGADLLLYGSFLAAGEELRIDARVVRAETGEIAATARAEGPAGRVL
                     DLERELSARLAAALGVPASPGPSVRTSPGPSSLDAARAYYRGLDLLDAGRYAEALEYF
                     AQATRLDPLYLKPGRGIEDAYRYLKDFRKQRQRREMNALVEDISALSARITAPTFFSF
                     GDAVMNPARFGFKDQAAVTEAYRAHPYRMAGDTPVQAVWNLQNLLHELGRSASENFGD
                     ETLADRCRDEVLRWSDAADSAYPRDPFLPEVLYQKIFVHVDREDWQALRSLCERLMSD
                     YPDYRMMWAVEDFYERALEELGG"
BASE COUNT         2986 a         6223 c         6107 g         2991 t
ORIGIN      
        1 gatgaggctg ggtatggccc gcacgatggt gacgtagacc gtgcccgccc cgtccgggaa
       61 gcggtccttg aaccgggcca taaggattcc caggcccagg cccagggcca tgctcagggc
      121 cagggccgcc aggccgaacc agagggaata gggggccttc tcgccgatga cgtccagaac
      181 cgggctgcga gggtagacgg tgattgaacg gcccaggtcg ccccgggcca ggccggacag
      241 gaagcgcccg aactgggtga agggatcatc caggaccccc aggttgcgaa ggtaggcggt
      301 ccgggcgtcc ctgtccatgt tcacgtagtc ctcccgagtg aagtagccgt tcttgggcat
      361 gagccggagc aggaggaata ccgtgaggac cacgatggac acggtgatca gggactgtcc
      421 gagacgtttc agtacatagg cacccatggg cgatccttca aaaaaaaaat tactgaaaaa
      481 gcaaaccaca gagtcacaga gacacagaga gcgaccggcc ggtcggcgcc gaagcaagct
      541 tcggcgatcc caactccgac aagctcgacg aagaccgaag gtcttcgttg ccccgaggct
      601 gacaaatagt cagcctcggg ccctctgtgt ctccgtggtt ttattgttat gagaactact
      661 tcgccgcggc catctccttg tagaaggcgg ccttcagctg ctcataccgc ttggccgtga
      721 tgggatcctt ctccacgacc atgcccttga acttgaaccg ggtgatgccg aagccgcccc
      781 ggggatacgt gaagggaacc accttggtga tctggtaggc cgtgccgccc atggcccagg
      841 gcaggacgta gcagcggtcc aggtagaggg cttccgcctt ggcgaagatc gcgtagcgct
      901 cggacaggac ggtcttggac atggcgtcct ggaccagctt gtcgaactcg ggatccgagt
      961 actgcccgtc gttgacgccg ccgttcgtga ccaggcggct gagctgggcg gcggggtccg
     1021 cgtagccgaa gcggaagttg tcgtgcagga ggtcgaagcg tcggggctcc accacctcgc
     1081 cgaacacgtc gtcgatgcac tggcccaggg tgacctcgat gttctccttg ccgaaggcct
     1141 cctcgagcat ggccttgagc aggagagccc gcttggtttc caccgcgtcg ggcagggaga
     1201 cgtagaccat ctgcaggggg agcttgccgt ccaccttgac ctcggcgatg ggcttgtagt
     1261 ccaccgtggc cggggagacg cccttgatgg tccccttgcc gtccgtgagc tccgccacgg
     1321 ccttcttgaa atagtcccgg gccttggcct tgttgtagta gttgcccgcg gccttgatct
     1381 ccttgacccc ggtgtagtcc gtgtagtcca cgcccttctc gtcgaagatc tggaactccg
     1441 gcaccaccgt gttccggatg atctggccgg gcttgtaggg atcgtccagc tcgttcagct
     1501 tcacccggtc gatgccgtag tacagggcct tgcggaagtt caggttgttg acgaaggcct
     1561 tgaactcggg gttcgaggaa gtgaagttct ggacgaacca gtaggtgacg gagaagcggt
     1621 ccgccgaggg cggatccgag tagatgtact ggccccactt ggacttcgcc agggccttga
     1681 cctggtcggc cccgaggtcg gcccgggaga gctcaccccg ctggaacatg gccacctggg
     1741 tagtcgggtc cgggacgtac tggagggtga tcgtcttgac ggagatcttg cccgcgtccc
     1801 agtagtgggg gttcgccttg aggacgatcc gcttggagcg ctgccagtcg gagatgtagt
     1861 aggcgcccga gaagacgagc ttctccttgg agaccccgaa gtccaggccg acggaatcca
     1921 gaaattcctt ctccacgggg aggaagagct ccatgaccag gtaggacagg aagaaggggg
     1981 tgggcttgga gagcttgtag accagggtgt acttgtccgg cgcggaaatc ccgaccttgg
     2041 agaagttggc cagggcgtcc tcccgggtca tgtccttctt cttgcccgag tcgacatccg
     2101 agaggtccag ataatagccg ttgaggccgg cgatcaggtt ccgcacgtcc cgggagaaat
     2161 tcttgatccc gttcttggga tccgagacgt agcgcagtcc ttccacgaaa tcgtccgccg
     2221 tgatctccca acgggtcttc tggcccgagg agtccacgta catcaggccc tgccggagct
     2281 tgaaggtcca ggttaggccg tcgggactca ccgtccagga ctgggccagg gaaggcacga
     2341 agcgcccgaa gcggtcctgt tccaccaggc cgtcctgggt gttcgcggcc acgcgctggt
     2401 ccgtggtcgt gggggtgtag aaataccgga gggacgaacc ctccgacgcg tacacggcgt
     2461 agtaacggtc gacgcccgcg gcccaggcgc ccgcggtcgc cgcgagggcc atcaggaccg
     2521 cgatgatcat cgctctcgat ttcatgcctt cctcctagga atttcgccgg aatacggacg
     2581 gtgccggatc tcgagcctgt gcggggccat gccccgtttc attacagcaa gaaccgtacc
     2641 agcggcaggt catcggaaga gtttgatttc gatttccgcg ggaaggatgt ccacgaagac
     2701 gtgctcgaag ccgaactggt ccccggtcca accgcgggtc aagccttcct tccggccgag
     2761 gcggacttcc caggccaggg gcaggtcgtc atccccttcc tcgaccagga aggaccggac
     2821 gtaccggaac ccgcggtccg ggccccgaag gaggccttcg ggatggttgt ccaagagcat
     2881 gcggaaggcg aagtccgcga gggtcccgta ctccgagaga atgccggagg cctggggccg
     2941 ggagacgtcg aaggagggat ccccgggatc caggggctcc ttgccccgga actcgacgaa
     3001 ccagtccgcc gggcggaagg agtccccctc ggtgagataa tgaaggcggg catgccggat
     3061 ctcgaccctg cccccgggaa ggccccgaat cgcctcccgg aggtcgacga cgtacacccc
     3121 gccggcaccc aggatctcgg cccggccctc ggccgccctc cagaccaggg cggtgtcctc
     3181 ggcgatcccg aacccgggcc gccggccccc gtcctcgacc cggcaggctt ccaggagccg
     3241 ccccagccgg gcccgggcgt cgaagtgctg gtccacgatg ccgaccggga aaaatcccag
     3301 gccccggcgc acgtacaggg cccgggagat ttcgacatcc gcggcctcga cggcccttgg
     3361 cagggccagg gcgccgaaac tggtgccccc agcgatcatg ggatccgaca tcacggcggc
     3421 ccccgcgctg gagcccccca tgggagcccc cccctcgttg gcccggcgca taacggcgag
     3481 caggggggta tcggagccat ccggctccag gagggtgtcc gtcagggcgt tctggtcgcc
     3541 cccgagcatc cagatcccgt ccgcccgggc ggcccgctcc gcgaccgcgg gatcccgggc
     3601 tccgtcctcc cagccgggta ccgcggcggc tacgggcagg agctcgatcc gggaaggatc
     3661 cacccccagg gatgtcaggg agttacggaa gaattcgaat ccggatcggg ggactttcga
     3721 ggccgcgccc aggaccgcga agcgtcctcc cggcccgccg gcggccttca cgaaggcggc
     3781 gtgcacgtcc cgggcgcggg tacggaggtt gccgccggct atgaacagat cacctttgac
     3841 catcgaaggg cttcctcatt tcggcttttt tctggtcaat tggcgccata ttaacctttt
     3901 catggccgat tttatcgcga ggaaacggaa tacaatcccg cctcaatgga ccgaccggac
     3961 cgcccgccgg ccctccgccg taagccggct gccccggcgg ccccggccca tgaccagaag
     4021 ccccagggcg gccaagcgtt ccagccggga cctcgcggcg ccggtgccca ggccgaggcc
     4081 ccgggcccgg gccagggccg cgatggtgtc ccggcccgcc gcctttccct cggcctccag
     4141 ctccgccacc gcccccagga cgaagcggtc ctcgaagccc agcggggctg ccggaccggc
     4201 gtccccggag agggccggac ggtacggttc cgcctcgcgg ggggcgaagt actcgtgctc
     4261 cggcaggtcc gcggcggaga tctcccgatc ctcccggacg gccagcatgt aggtcagggc
     4321 gttccggagt tcccggacgt ttccgggcca gtcccgggac tccagggccg ccaggacctc
     4381 ggggctgacc gccacgtccc cggccccttc ctgctccagg atacggcgca cgagccgggg
     4441 tatgtcctgc ctgcgctcgc gcaggggagg tatgcggagc cagcccatct tgagacggaa
     4501 gtagaggtcc tcccggaact ggcccttccg ggccttgtcc aggaggtcct cgttggtcgc
     4561 cgcgatcacc cggacgtcca cacgcttcag gtccgccccg cccacccgca tgacctcctt
     4621 ttcctggagg acccggagca gccgggtctg gaccttgggg gagacgttcc cgatctcgtc
     4681 caggaagagg gtgccgccgt cggccagctc gaagagcccc gtcttgccgc ccttcttggc
     4741 ccccgtgaag gccccctcct cgtacccgaa gagctcgctc tccaccaggt cgtccgaaag
     4801 ggcccccagg tccacggcca ggaagggccc ggcggaccgg gcgctcgcgg cgtggatggc
     4861 ggaggcgaag agctccttgc ccgtgccgct ctccccgtgg atcaggatgg acaggtccgt
     4921 ggcggccagg cgggtggcga tccgcttggc cctgcggacg gcctcgctct ctccggcgat
     4981 gtcctccagg gtccacttgg ccacgtggcc ccggcgacgg tactcccggc ccagccggga
     5041 agcttcggga ccctcgccgc ggaagaccgc cacggtgtgc ccgccctccc caaggtcgaa
     5101 gcgccggacg accagggtgc cctccgggag cttgaaggtc ccctcctcct ccccgcagcg
     5161 gcagtccagg aagcccagaa gctcccggtt gcggatcagg gatcccaggg ccgcccccgg
     5221 accgctgccg ggccggagat ggaggaggtc ccggagctcc tcgttgcaga ccgagacccg
     5281 gccttcctcg tcgtagacca ggatgccgtg gcgcagggaa tcgatcacgg agccgaggtg
     5341 gccggacaga cggcgggcct cctcggtgga ccgggccaga cgacgggcca ggttgacgat
     5401 cttggccagg tagcgccggg agtagccgcc gatatccgtc tccgccatcc ccaggcgctt
     5461 caggatctcc gccaggcttc cgaaatccag gatccgcacc ccgatgtcga tcacctcgtc
     5521 catccccggg gggaccagat ccggttcccc cgccaccacg gcgatccggt atgaccgcgg
     5581 aggcggcgga tcctcggggt tccagggaag ccagtccacc ccgtccagtc ccaggtcccg
     5641 gagggaatcc acgcagtccc gggccgtctc ggggcggtcg ttcacgaaga ggaccgggag
     5701 gcccgggggc agggccacca cgcgctccag ggcgtcgcag tccacgaccc gccgggccac
     5761 caggggctcc gtaccgacgg gaaggaggcc cgacgcctcc agatccttcc ggacgaggtc
     5821 gctggaaagg accacgaggt ccccctcggg aggctccgaa agaccctcgt ccacccagta
     5881 cggcacgatc tccgcccggc cttccacgta ctcggccagt tgacggacca ggacgcgggc
     5941 cgtgctctcg ctgcccgcga ccacggccac cttcacgggg cggcctccag ggggtcgatg
     6001 ttctcgaagg tcccggatac gagctcctgg ccgtcccgga tcatcacacg gccccgcgcc
     6061 acgacggaca cgggcttgag gtcccggtcc aggaccgtca ggtcaccgtc caaacccggg
     6121 gcgatccgcc ccttgcggac gagcttgagg accgcggcgg ggttggccgt gatcggtagg
     6181 agggccgttt ccagggggat gccccggacc ttcacggcct cccgcagggc ggaaagaagg
     6241 ctggcgcagg tgcccaccag gagcccggaa agtttcccgg cggcgtcgaa ggagggcagg
     6301 ctgccctggc cgtcgctgga ccaggtgatc cggtccgccg ggaccttccg ggacaggcag
     6361 agggcgaagg actccgcggc ggaactctcc ccctcctcca ggaaccgggg caccgtgctg
     6421 gtcgtgtagt ccgcataccc ccccgccagg gtccaggcca cggactgttc cagcacgatg
     6481 gggttccggt tgcagtgggt ggggaggaac tgggtccggg gcaggtctcc gggggccacg
     6541 atgcgctcca ggggctcgaa tcccgcaggg gcgtcgccca ggtggacgtt cacgactccc
     6601 gccttgccgg agagcatgcc cccgaggcgc gcttccgagg ctatgcgggc cagctccgcg
     6661 tccgtgggct tggaggagcg gtgatccgag atcgccagct ccccgatgcc taggatgggc
     6721 tcgatcatca tgatgtcccg gaccggatcc cccgtcacgg tggtcagggg taccctgtag
     6781 gaacccgtgt atccgtaggc ggagatgccc tcttcccgca ggccgtagag cttggccacc
     6841 agggcttcca tgctccgggc caccccgtcg gtgcccagga ccccgaccac ggtggtcacc
     6901 ccggcccgca gggcgcccga caggaccagc tcgggggtgc gggtccggta ccctccctcc
     6961 ccgccgccgc cggtcacgtg gacatgggaa tccacgaaac ccggcacgac gaaccggccc
     7021 cgggcgtcca ggacctcgca gggagccggg aggccggcgg ggtcgatccg atcggcgatc
     7081 gcgacgatcc tgcccgcggc gatgaggatg tcccgcgggc cgatccgttc gggtgcgtaa
     7141 aggtcggcgt tctggacgag cagcagcatg gtgtagcttc ctttcgatcc gggatggaaa
     7201 tcttgtagca atcatcgtgc cagttccgtg gacggtggtc cgttaacagt gaacagtagt
     7261 acgaagcaga cgggttcggg cgagatcgtc cccctcgacg cccggcgcgt tcggccgcgt
     7321 cggatcgaag tataatctcc ctgcccggga ctccgccatc gtacggacaa aagagcggcc
     7381 gtccactgtc gactgtccac cgactcctga ctcctgactc ccgactcctt ctatgctatc
     7441 ctccgggtat ccgacgaccg ggagccgaac gatgcagcat gtcctgctgg ccttctatat
     7501 cctgtttttc gccacgggct tcatgggggg cgccgccctc ttcgtcctgg gcctgaggat
     7561 ccgcagccgg ctgatccggc ccctcctggc cttccaggcc ctcttcctgg tggggctggg
     7621 gctggtcgcc ctctacgcct atctgtacgg cctttggggt gccgtgccgc ccaccctggc
     7681 cctggtgctg ggaaccgtcc tgaccgggat gaacgccgcc atctacgccg tggccatcct
     7741 gctggtccgg aggatctcac cgcccgcgtc ccggagaaag gcctatcccg cggcggcgga
     7801 gatcctggcg ggcctggtga tagcgaagag cctggcgagc ctggtccttg ccctggcggg
     7861 cgcctcccgg cccggggctc gagccctgct ggatacggag gcctggagcc tggcggggta
     7921 catcctgacc ggcctggcca tggccgcctt cggggtgacc gcccggggtc ctcttcctcc
     7981 cggggaagcc ccggccctgc ggcccctctt caaggcctac gggacctgcg ctctggcctt
     8041 cgcccccctg ggtctcgtgg agtacgcggt ggagaccgcg ggtcttcccg gcctgccctt
     8101 cctctccctg gaccacctgt tctacctgtc ctggaacatc gtgtccatga gcgcggccgt
     8161 gcggctgctg aagcccgggg acgcgggggt ccccgctttg gattcggttc ccgcggaacg
     8221 gatcgaggcc ttgggcctct ccggccggga ggcccagatg gcggtcctga tcgcccgggg
     8281 cctggccaac aaggagatcg cggcggaact gggtatctcc ccggccacgg tgcgcaccca
     8341 catctataac ctctaccaga aggccggggc gcgcagccgg gtggagttga tcaacaagct
     8401 ccgggcctga ggccggccgc cgacgggtcg gccggcggcg cctcagtccc tccgagcccc
     8461 ctcagggggc aacactggcg cccgtgccga catcggcctt gacggaaagg taggccggaa
     8521 gggtaagggc gacgactgcc gccagggccg ccgcgaccac tacgggcgcg acgagggatc
     8581 gcttctcccc cgcccgggga cccgcgatcc gggcgcggat cacgcaggtc gccccggcca
     8641 ggaaggcgca gatcacggtg gcgatcggcg cgaaggtgca cagggacctg tagcccagga
     8701 cgttctccca acgggcgccc gggttcggga ccagggtcag gatcgccgcc agggtaaaaa
     8761 ggccggacaa gcccaggagg gcgccgtaga gagccggatt ttttggttgc ttgcgttcca
     8821 tgtcgaaatc tccttgacaa agcgatttag ttcaccggcc cggggccggt ccgttccgga
     8881 aatctcccgc ccggggcggt atcgtccacc cccgccgctc gaacaggtcg gtccaccgct
     8941 cccgggcttc ggggctgccg tcctcgtcca ggccggagag caccccgtac atcaggagag
     9001 agcccgcgga gcggaggcgg ctggccgccc cttcctcgct ccggtccttc gcgtggccga
     9061 aaagcatggc catgaccatg cccatggtgt aggtccacag ctggtcgaag atccgatcca
     9121 gctccccttc tccgagagag gccaggaagg cgtcctcctt catgcggcta cggacttccc
     9181 gccggtggcg ctcgagctcc ccgtccatcc cggcccggta gagggccagg aagagcctgg
     9241 gttcgtcccg ggcgaagagc acgaggccga tgccgctgtt caggaatccg ctgtccgtac
     9301 gggggcgggt acccgacgcg cgaagcagcc gggaagcctc cccgaggacc caggcttgga
     9361 ggctctccat ggattcaaat gctccgtaga tcggggcgac ggaggtcccc aaggcggccg
     9421 ccagcccccg ggcggtgagg gcatcccagc cttcccggcg gaccaggtcc agggcggcgg
     9481 ttccgatatg ttccctcgag aaggatacgg gtcttggcac taggacttcc tccgcataag
     9541 ctaactcttg ttatattaca tatgttaggt tatgtcaatc ctttgttctc acatcggcgg
     9601 gatcccccga aagcacgggg ccgctcgaag gagcggcccc gtgctttccc ggagcagggg
     9661 attcagtcgg cggccctgcg cgcctcgtac gcggagagga tccgatccag cgccgcgtcc
     9721 agggtagccc gggggcaggc caggttgaac cgctggaagc cgctccctcc ggtcccgaat
     9781 ttgatcccct cgtccagcca aaccttggct ttgtggagga agaagtcctt gagctccggg
     9841 tcggacatcc ccaggccccg gcagtccgcc caggccaggt aggtgccctc caggggaagg
     9901 acccggatcc ccgggatgcc cccctccagg acggtcttga gtcgaaggta gttgccccgg
     9961 acgtaccgga tcagctcctc cagccagggc tcccctttcg tatacgcggc ctcctgggcc
    10021 gcgatcccga agacgttcga ggtgcccaag ccgaggttgc cggccgccgt ggaaaagccg
    10081 tcccgcaggc gacggtccgg gatcaccacg ttggccgtgc acaggcccgc caggttgaag
    10141 gtcttgctga ccgccaggca cgtgagggac cgggacgcgg cgtcctcgga gatcgcggcg
    10201 aagcaggtgt gccgccgacc gggcatcacg atgtccgcgt ggatctcgtc ggaaaccacg
    10261 accaagttct tccgggcgca gagctccgcc agggccgaaa gttcctcccg ggtccagacc
    10321 cgccctacag ggttgtgggg gctgcaaagg atgagaagct tggtccggga atcgatcttg
    10381 cgctccaggt ccgggaggtc catgacgtac cgatccccct cgagccgcag gggattgtcc
    10441 aggagccggc ggccgttgtt caggaccgcg gccccgaagg ggtagtagac ggggctctgg
    10501 atgaccacct tgtccccggg ccgggtgaag gccaggacgg ccaggttgac ggcgggcacg
    10561 atacccgggg agtagcagat ccactccctc cggatctccc agccgtagcg ccggcgggtc
    10621 catcccgtca gggactcgta gaacccgtcc tgccgaaccg ggtagccgta gatcgggtgt
    10681 tcagcccggg cccggatcgc ttcctggatc tccggggcca cggggaagtc catgtccgcg
    10741 acccagagcg ggatggcatc ctcctcgccg cagagctcgc ggcgggcgct ccatttcacg
    10801 catcctgtgg gaacgcggtc cgggaccgta tcgaaatcgt acatgccgcc tcctgcccgc
    10861 ccatagtggt acgaagcttt ccaggaaggc aagaggcgtc gtacaacgac cgcctaccgc
    10921 agggtggtcc accaggtcgc ccggatcgag gcgccgaggt cgaagcccga caagccgcga
    10981 ccctcgaagt cctcataccc gggggcgagt cgacaggagc cccagagccg ggccgaccat
    11041 ctgccgccca gggacgcttc cgccccgagg gtcaggatgc cgcagaagct cttctcgacc
    11101 ccttggccgt cgaaggcgcc gctcccgtcc gcctcgtcct tggcgatgcc ccccagaccc
    11161 aggcggacgt agggaacgat tcccgcggcc cccgggccca ggcggaagcc caggctgccg
    11221 ccgtagacga gagccccctg ttggatcagg tccgggctac cgaacttgag ggggatcagg
    11281 tgcaggtagc tcccgagctc cacccggccc aggaagattc ccgattccag ggaggccaga
    11341 gaccggatct ccccgcccgc ggcggtggcc ccgccgccga tctcggcata ggacttccag
    11401 gtcagtcctt ccccggccca ggccggtccg gccgcccccg cgaggatcag ggccgccagc
    11461 accaggaccg gcgccttgcc gaaacgggga aggaacatcc ccgtccgggc cttgtaggcc
    11521 ctccactcgt ctccgaatcg ttcttccatt tccgtttctt cctttcgggc cagccggacg
    11581 tacatcgacg ccaggatcgg ccaaagaatc aggagcggga tcgtcgccca ctcgaacagc
    11641 acacccaggg atacgagcag aaagcccgtg tactgcggat gccgcacgta ccggtagaac
    11701 ccctccgcga ccagcctccc ctgtccttcc tccctgctcc agtactcccg gtggatgcgg
    11761 gcccagccgc ccaccaccag gaccgctccg gccaggacgc aggccaggta gacgtagtgc
    11821 ccggtcatgc cgaaggttcc ggacagggtg tgcccccaga gaaccccctc cggcagggtc
    11881 ttcccgaagg cccagagcac gaaatacagg gacaggggcg ccccgaacat ctccagggca
    11941 aaggccacca cgaagcccag gaagaccccc gcgggcttgc ggtccgcctt acggtagaag
    12001 ggcacgaaga gcaggaaggc gccgaagagg gcggcccaca cggcgaccgc ccaccaggtt
    12061 ccgaaatgcg accccacgtt ctcgtccatt ccgacgctcc atcgaaggaa ggatgcggga
    12121 ggcggcccgg ggcccatcca cccgcggcgg cgatcaccgg atccttcgac tccggcgccg
    12181 tccgccgagg gagaggatag ggattccgaa gacggatggg gatcggacga agctatgaaa
    12241 atcgggtgga aagcctacgt aatatgtatc agttccggtt tatgggccgt catgaggtcg
    12301 cgcggaaccg cccggaccgc ggcgccggag cctaggcggt cgagtcatcc ttccacgccg
    12361 gaaggaggac ccggaacagg gtgcgcccgg gctcgctctc cagctcgatc cgacccccgt
    12421 gtttttcgac gatcctgcgg cagatgtcca accccaggcc ggtgccctct ccgctcttct
    12481 tggtggtaaa gaaaggttcg aagatctttt cccgcaggtg ggcgggcacg ccggggccgg
    12541 tgtcccggac ctcgaccacg atccgggaat ccccttcccg gacgctcagc cccagggtcc
    12601 cgcggaaatc catggcctgc agggcgttct ggatcaggtt catccagacc tggttcagtt
    12661 ggttccggtt gcccttcacg agccccgggc acccgtagtc cttttcgatc cggactccgt
    12721 acttggtctt gttggtgtag agggtgagca ggctgtccag ctccttcggg atctcgaccc
    12781 gggaggaggc gtcctcgtcc tcctctttcc ggacgtagta aaggagggca gacaccacgt
    12841 tggagcactt gtccgcggcc acggcgatca cgtcggttat gttcttgatc gaggagaggg
    12901 tatccagggc gtacacggct tccgggcaat ccggatgatc ccggaccagg gcgggaatcg
    12961 aggggtcccc gtccagacgg aggtcgagca gggcatccag aagcctgtcc gggatcggac
    13021 ggccgcagtc cgccaggcgg gccgcaagcc ctttccgcct tcggaaacgg ccggatgcct
    13081 cttcctccag gggccgcgag cccagggaca atttcaaggc cgcattgaac caggtcagga
    13141 cttccgggga gagcccggct acggtccggg acgcctcctc caggcgattc ccgatcaggt
    13201 ggatcaggga ctggttggac gagacgatgg cccccagggg ggtgttgagc tcgtgggcga
    13261 tgcccgcgat cagctggccc aaggccgcga gcttctcgga ctggaccagg gaatcctggg
    13321 cctcgtggag ggtttccagg gtggcctgga gttcctcgtt ggctttgacc aggctggagt
    13381 tcatcctgtc cagttcccgg gttcgatcca ggatcctctg ttcgagggtc tcgttcaggg
    13441 ccttgagctc ctcgtccacc cgtcttcgct cggttatatc ttcatataca ccgaggatcc
    13501 cgtagattcg gccttcggga tccttgagag ggatcttgga agtccggagc catttccggc
    13561 ctccgtcgga gctcatgggc tcctcgtagt tgatcttggc ctggtcctgc tcgactaccg
    13621 accgatcgtc cgcccggtac gcctcggcct gatccttcca taacaaggag aaatcgtcca
    13681 gaccgatcac gtcctcgggg cgatccttcc cggcgtcctg ggcgaaggac cggttgcatc
    13741 ccaggtaccg cccggcccgg tccttccaga atacccggac ggggatggtg tcgatcacgt
    13801 cgttgagcat gcgggtggat tcccagagcc tctgctccat gagctttcgc tccgtgacgt
    13861 cctcgtagat ccccaaaact ccgaacaccc gtccctcgtc cacgaggggc accttggtcg
    13921 tatcgatcca cagccgggct ccgtccgctt gttgaagggg ttccacgatg tggcgtttcg
    13981 gacgcccgct ttccacgacc tcccggtcgt cggcccgata ggcctccgcg tcctcggccg
    14041 tccagggaag gtcgaaatcc gtcttgccca ggatccgggc gggatcctcc aggcccacgt
    14101 cccgggcgaa ggcctggttg catcccaggt aacggccgtc ccggtccttc cagaagaccg
    14161 attggggaat cgtgttcagc accaggtcca gtgtcgcccg gctctttcgc aggtcctcct
    14221 ccgccgcctt cttgtccgtg acgtccgtat gggtcccgat catccggagg accttcccgg
    14281 ccccgtcccg ggcgacgacc ttgccccggc ctcggatcca catccaggtc ccgtccttcc
    14341 ggaggaagcg gaactcgatg tcgaactgga tcacccggcc gtcgatgtgc gcctgggagt
    14401 gcgcgaggac ccgctccagg tcatccgggt gaacccgctt ctcgaactcg tcgaacctgt
    14461 gggggaagtc tcccggttcg tacccggcga tcttgtagta cctcggatcg aagtagaccg
    14521 tgttgctcgg gatgtcccag tcccagatcc cgtcgttgac cgccagcatg gccaggtcga
    14581 agcgttcctg gctgagccgg agggcttccg cggccttgtg gcgcagggcg gaagaccgga
    14641 taagggcgag gatcagcccc agctgcagga cgatcagcgc caaggagccg gccaggaagg
    14701 tccgggtccg gtcccggagg gtgcgggcgg tccggttctt ctcggtgatg tccgtgaccg
    14761 tgaagacgac cccctcggag aggtccgcgg tatccagggg ggtcgaggag aggatcacct
    14821 ccaggatttc cccttccttg gtcctccagc gggtctccac ggtcccggtg ccccgctccg
    14881 cgatctgtcg gtacttctcc cggcccacga actcgtaatc ctcgtcggca ggatagaaga
    14941 tccgggcgct cttacccagg agttcctccc gggagtatcc ggtcagctgg acgatgtagt
    15001 cgttcacctg gacgatgacc cggtccacga ctacgccgat ccccccgggg gcgctgcgca
    15061 ggatgctcga gagcatgcgc tccgcctcga gttgggataa ctccatctcc ggatcggccg
    15121 gccggtcctg ggcgcgcgcc gcgggcgcga aggcggccag caccatcgcg gccgcgaaca
    15181 cccggaatcg gtacggcacg tcggtaccta cgccggatcc cggaacttgc cgcaatggcg
    15241 ggaggcgtca ccggccgaga tctgccggaa ggagtccttc tcggtccgga aggcctccac
    15301 gacggccggg tcgaaacgct tgccggagtc cgcctcgatc tcccgtgcga cctcgtcgaa
    15361 ggggagggcg tccttgtagg gacgtttggt gagcatggcg tccaggctgt ccgccacggc
    15421 cgtgatgcgg gcggagagcg ggatccgagc cccgaccatc tcggccaggt accccttccc
    15481 gtcccagcgc tcgtgatggt acaggatcaa gtccttggcg aattcgtcga tgtcgatgga
    15541 tttcagcagg ttcgtgccga tctcgacgtg ccgtttgacc acctcgaact cctccggggt
    15601 gagccttccc ggcttgtcca ggatctcgtg gggtatgccc gccttgccga tgtcgtgcag
    15661 gggggcgtag gtatagagct ttcgcacctc cgcctccgca agacccagct tccggcccag
    15721 gagggcggcg tagcaggcca cccgctggac atggcagaag gagtccgggt cccagtcccg
    15781 ggcctggttt tccagggcgg cgatcagggc cgtgttgagt tcctccacct tccgacggag
    15841 cagctccagt tccgcctccc gatcgtagag ttccgcggcc ctccggaccg acatgaccag
    15901 gtcccggcgt tcccagggct tgtggatata gcggaacaac ccggcctcgt tgaccgcctg
    15961 ggctatggcg tcgatatccg aataacccgt caggaggatg ccgtacagcc ggggaaaggc
    16021 cttccgggcc tcccggagga ggatatgccc cttcatgccc ggcatccgct cgtcggtaac
    16081 caggaccgcg ggctctccgc cgtcctggct tatctccgcc aggagtgaga gggccgtctc
    16141 cccggaatac gcggcttcca cccggaggtg cgggagttcg tgccggagtt cccgcttgag
    16201 actctcggtg atgatggcct cgtcgtccac gcacagcacg tatcgttccg ccataccgtc
    16261 tattctatac atgcggggcg ggacttcaag gaaaacgcac gttgaacgtg aagtaccgta
    16321 tggccggggg tctgtccttc ggggcggcct tcgggtacgc ggcggcgacc cggcgaaata
    16381 agagaagggg ccccggagcc gggcctggct cgcccgaccc ggctggatcc ggagcctact
    16441 tgatcccgta gaccttcacg accgcccggc cgtccttgag gcggccctcg gtccggtaat
    16501 ggaaggttat cgtccggata aggcgcctcc cgccctctag atcgacggcc cgggaccggg
    16561 tcccctccgc gaagcggtgc cgcaggggga tctcgtcctt cctgccgtcc ccgtaggtca
    16621 ccacgatgcg ctcgatctcc acgtcgttgc cttccgcaag gaagacgagc ctgcgcaggg
    16681 ccccttcctt cagtcggacg ggaagcacgt cgaaatccgc cttgaagttc actttcaact
    16741 cgccgatgag ctcggccttg ccgatgaggg tccgaatctc ggaatcgctc tgggcgaagg
    16801 ccgacccggc ggccaggcac agcagaacca cgtttagaaa tcgcttcatg gcacctcccg
    16861 ggcggggaat ataccgcgat ccggggccgg aaaccaccgt gttcctaccc tcccaactcc
    16921 tccagggcac gctcgtagaa gtcctccacg gcccacatca tgcgatagtc cgggtagtcc
    16981 gacatgaggc gctcgcacaa ggatcgcagg gcctgccagt cctcccggtc cacatggacg
    17041 aagatcttct ggtagaggac ctccggcagg aagggatccc gggggtaggc ggaatccgcg
    17101 gcgtcggacc agcgcaggac ctcgtcccgg cagcggtccg ccagggtctc gtccccgaag
    17161 ttctcggagg cggagcggcc cagctcgtgc aggaggttct ggaggttcca gaccgcctgg
    17221 acgggggtat ccccggccat gcggtaggga tgggcccgat aggcctccgt gacggcggcc
    17281 tggtccttga agccgaaccg ggccgggttc atcaccgcgt ccccgaagga aaagaaggtc
    17341 ggggccgtga tccgcgcgga gagggcggag atgtcctcca ccagggcgtt catctcccgc
    17401 ctctgccgct gtttccggaa atccttcagg taccggtagg cgtcctcgat gccccggccg
    17461 ggcttgaggt acagggggtc cagccgggta gcctgggcga agtactccag ggcttccgcg
    17521 tagcggcccg cgtccaggag gtccagcccc cggtagtacg cccgagccgc gtccaggctg
    17581 gaaggcccgg gcgaggtacg gacggacggg ccgggcgagg ccgggacgcc gagggcggcc
    17641 gccaggcgcg cggagagctc ccgctcaagg tccaggaccc ggcccgcggg gccctcggcc
    17701 cgggcggtcg ccgcgatctc cccggtctcc gcccgcacca cccgggcgtc gatccggagc
    17761 tcctccccgg cggccaggaa gcttccgtac agcaggaggt cggcgcccaa gagccggccc
    17821 accttcgggg ccccggcggg atcgaccagg ccggagaggg ctagttcctg ctcccgcagg
    17881 acccgctcca gctcctccct ctccacgagg gtgaaggccc ccgacgcccc gaggtccccg
    17941 gccagcaggt ccgcgaggcc cttggacagc cacgccaggt ccgcggagcc gcgggtgtcc
    18001 gtgaagtaga ggaccgaaag ccggggcttc ccggtccgcg gctcctgggc ggacaggccg
    18061 cccccgggga gggaccaaag aatccaggcg gtcaggaggg ccgcggcggc gggaaggccc
    18121 ggtcggcgcg gcggggcgac gaagttcggc atgggacgag tatgcagggt gtccgggagc
    18181 ttgtgaaggg cggcgggtgg aaatacgggc cgcggtcgtg gatactatcc ccatgagcga
    18241 aaaacccgat acggagccga cggacccgga atcgcaggtc gttccggtca ccgaggcgaa
    18301 cctggag
//
LOCUS       DAOTEI010000059        18304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2040_length_18304_cov_5.147570, whole genome shotgun sequence.
ACCESSION   DAOTEI010000059 DAOTEI010000000
VERSION     DAOTEI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 18304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18304
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2040_length_18304_cov_5.147570"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="P5313_07555"
     CDS             complement(<1..1689)
                     /locus_tag="P5313_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRY80255.1"
                     /translation="MSDTQRVLRDTFLTNRSDFAVAIGAVAVVLMIIIPIPTVLLDAL
                     MAFNLTISLLILLIVLYTRKATEFSIFPTMLLVTTIFGLALNISSTRLILTQGSRFDG
                     RLIRAFSTFVVGSGGTEGLVIGFIIFIVIIAVQAIVITKGATRIAEVAARFTLDGLPV
                     KQMAIESEFNSGAITEEEARRRKDEVQREVDFYGQMDGASKFVSGNVKVGIFISVINM
                     LGGFVVGMVIHSESFQTAIEAYTSLTIGDGLLSQLPALLVSVATGIIVTRANSQGTFG
                     GDVTAQFSQNARIYWIGSAVLAALAFLPGFPWYVLLPMAASMAWLAFRLSRKQLRDAE
                     AAKAHPAGAKAKGESAQELSPVVPLDPISLELGYGLIPLVDRDKGAELLERITRIRKE
                     SALDMGLVVPRIRIIDNMRLEPSEYCFKIKGVEVGRGVIRLGYYMAINPGGVTEEIPG
                     EKTRDPAFGLSALWIAEDQRDKAERAGYTVVDAPSIVATHLTEIIKRHASEILGRQEI
                     QSILDTLRKDYPAVVEDAQKHLSLGEIQKVIQALLREQVSVRNMVVILETLADYA"
     gene            complement(1728..2915)
                     /gene="flhB"
                     /locus_tag="P5313_07560"
     CDS             complement(1728..2915)
                     /gene="flhB"
                     /locus_tag="P5313_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRY80256.1"
                     /translation="MTALAPDLRAYPAPPRVLSPADIHLQWFASPEEEGRTEEPSEYK
                     LRKAREEGRVAKSQDLVAAIGLLLTAVTLAILAPWMLQTLREMIVRFLKLSTESDIVT
                     DGALAAGLFFRYLLRLLLPLASVGVLAALFGNILQVGFLFSVKPITPDFKKVVPRLGQ
                     YFQRIFSMEGLYNLGKSILKIAIISTVAWILVNGELERLRNLYMAPFWTSVKLVSGMI
                     LKLILAVAVTLLALSIPDYMFQRRQFLERMKMTKQEVKEERKMHEGDPQVKGRLRERM
                     RDLLTRNMAVNVPKADVVITNPTHFAVALEWDRESMAAPMVTAKGMDEAALRIRRIAE
                     DARVPVVENRPLARALYAETEIGQTIPEKYYQAIAAVLAHVYALSDKGRRYARAAGAE
                     DLA"
     gene            complement(2912..3691)
                     /gene="fliR"
                     /locus_tag="P5313_07565"
     CDS             complement(2912..3691)
                     /gene="fliR"
                     /locus_tag="P5313_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRY80257.1"
                     /translation="MFNEILDGADLFFLIAVRVLAMVETAPLLSSDGIPQAAKIALSG
                     LAAAAVFPWVRDAGYPIPDTIGPYLALLAGEALLGIITGFFLVAVQAAFVTAGQFFSL
                     QMGFGASEVYDPLAEIEIPLMGQYLNIVAMLVFVQVGGFRNLFLIGVRGSFQALRAVD
                     LVMRRDGFFEYLFGAVSLLFKNAMTLAFPILGTLMVISVTMGLLSKAAPQMNLLTEGF
                     PLNIGVAFLLLFGSLPFLMEAFARLIDAGFLDLARMLGGTG"
     gene            complement(3696..3965)
                     /gene="fliQ"
                     /locus_tag="P5313_07570"
     CDS             complement(3696..3965)
                     /gene="fliQ"
                     /locus_tag="P5313_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRY80258.1"
                     /translation="MDIGDAVNLMRGGILEIVLISAPILIVALVVGLIVSILQATTSI
                     QEQTLTFVPKIASILAVLAILGGWMLTHLAQYTRTLFELIPMAAR"
     gene            complement(3975..4658)
                     /gene="fliP"
                     /locus_tag="P5313_07575"
                     /pseudo
     CDS             complement(3975..4658)
                     /gene="fliP"
                     /locus_tag="P5313_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968982.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(4873..5481)
                     /locus_tag="P5313_07580"
     CDS             complement(4873..5481)
                     /locus_tag="P5313_07580"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRY80259.1"
                     /translation="MAVLCVLCPSAFAQPTPGTDVRGTVPSAGQGSAAPAAETDLVLA
                     ESSETANGGNRNALSDSAWPYFLRMLLTLAFVLGAIWLTFRFLRKAGRPRIAEDAFVK
                     VLASVPLGTGRWLYVVSLGAKAFLIGATDSSLNLIREVEDPELIDEMKLRAATAPQAD
                     TRDFGTLLSGLLKPVSKHRADSAKGIPGTEFLARQRDRLKRF"
     gene            complement(5654..6775)
                     /gene="fliN"
                     /locus_tag="P5313_07585"
     CDS             complement(5654..6775)
                     /gene="fliN"
                     /locus_tag="P5313_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRY80260.1"
                     /translation="MSDGALSQEEIDSLLAGLDTGGGGGKTAAAPGSSGGGNVSREAQ
                     FFKSILEGTAGSQSQNLSMITGLDVTVGAPQVDQVQRDAVLQSVPEEVLAVQIDMTGG
                     IPGEHLFILSKETAVRIASLANKDDSLTDLDDMAISAVSECVSTLTGTLITVFTDRTG
                     NRSLQSSSAEGNYVPKAMVRLPSGDFIRVVWPVNIGGKPEQFVETFSLSFVRDVVAAA
                     GGGGAPAQPAMQAPAMGGMGGMNAQAMGMYSQPMGGMFAGMANSMGGMSPPNVQPVQF
                     ANLAMQSAPPEAGNIGLIMDVFMEMTVELGRTRKLIKEILGMGEGTIIELDKLAGEPV
                     DILVNHKLIAKGEVVVIDENFGVRVTEIVSPMERMSDMS"
     gene            complement(6768..7802)
                     /gene="fliM"
                     /locus_tag="P5313_07590"
     CDS             complement(6768..7802)
                     /gene="fliM"
                     /locus_tag="P5313_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRY80261.1"
                     /translation="MTEVLSQDEIDQLLTAINAGETDVEAIRPTTDSRKIKIYDFKRP
                     DKFSKEQIRTVQIMHETFARLTTTSLSASLRTLVHVHVASVDQLTYEEFIRSIPTPTT
                     LAVVSMDPLKGQAILEIDPSVTFTIIDRLFGGSGEGSKLNRELTDIEHSVMEGIIVRI
                     LGNMREAWTQVIDLRPRLSQIETNPQFAQIVPPTEMVVLVTLETKVGEIEGMMNLCIP
                     YLTIEPIVSKLSAQYWYSSVRRGATTENLNILKEKLSTVEIPVIAEIGKIQVSVRDVL
                     SLRAGDVVRLYNVKVDDPMVLNVGNKKKFLCRPGVIGRKMAVQITKKLEDVGQEEFEE
                     LTSEEGESYE"
     gene            complement(7860..8414)
                     /locus_tag="P5313_07595"
     CDS             complement(7860..8414)
                     /locus_tag="P5313_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRY80262.1"
                     /translation="MGDSFEEQGMNLNEDGGSAPAPVRAKGGGAGILRLLMWIGIALG
                     AVIFVVTVVIVTVNIVNKQGKPMTQVALGESYQRASPEYEYFTTLGEIRTRTIDNEPS
                     SIVVKINLGFEKGDKDTPTMLTARIHQMRDRLRNYFSSKTADYLAPKNEQRLKEEIRE
                     DLNNMLSKPSIKEVLFEKLDVIKM"
     gene            complement(8547..9278)
                     /gene="motB"
                     /locus_tag="P5313_07600"
     CDS             complement(8547..9278)
                     /gene="motB"
                     /locus_tag="P5313_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRY80263.1"
                     /translation="MAKKQRKEIGGGTGEWIVTYSDMVTLLLCFFVALFDVTDVDITQ
                     MQVLISSMNNIGMGASTGGNTLSVGKLAELGNTVSTLPSMEKGRLLATAKKKATSLFQ
                     PEIKSNKVRISTDERGIVISLASDAFFRPASADVNIEETRTMLVRLAELLKSDELGGR
                     KFRIEGHTDATSTDPAGPWRSNWELSAARSLNVLHYLTDFGADERRFQVAGFADTVPI
                     ASNDTEEGRAYNRRVDIIILDEAHL"
     gene            complement(9281..10063)
                     /locus_tag="P5313_07605"
     CDS             complement(9281..10063)
                     /locus_tag="P5313_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HRY80264.1"
                     /translation="MDLGSLIGLIGGIACIILAMYTSGGQVGTYIDIPSVFMTIGGSY
                     CALLLNYSLKESIGLFSILGKCFRVEEFGESKIVEKLYELAEKARREGILSLEEVIAE
                     LDDKFMQSGLRLVVDGTDAEIIRNLMENELNAMNERHARWLKMIDNWSKLAPGFGMLG
                     TVQGLIAMMKNLEDKSRIGPNMAVALITTYYGAIMANFLLTPMMGKLAAKDAMETRLR
                     EMIIEGVLSIQAGDNPRILLVKLTSYLEPADKKELIDKYGKD"
     gene            complement(10066..10263)
                     /locus_tag="P5313_07610"
     CDS             complement(10066..10263)
                     /locus_tag="P5313_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HRY80265.1"
                     /translation="MIELTQLSGKTFWINPHQIEYIEKNPDTTLIMLSGKRIVVLEGV
                     PEVLGRIVAYRRQIGAFKNEE"
     gene            complement(10396..11787)
                     /gene="flgE"
                     /locus_tag="P5313_07615"
     CDS             complement(10396..11787)
                     /gene="flgE"
                     /locus_tag="P5313_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRY80266.1"
                     /translation="MMRSLYSGVSGLQNHQVRMDVLGHNIANVNTTGYKKNRVNFQDL
                     LYQQMSGAARPNEEVGGVNPKEIGLGMSIASVDTIHTQGSLQTTGVMTDIAIQGNGFF
                     VLQKGDSRFFTRAGAFGLDRDGRLVNPANGYRVQGWNAQVVNGREILNTSGALEDLVI
                     PVGSKDPAKATEQVYLACNLDKRVPEIPEGASEETILRGTWRVEEKIYDSFGIEHILR
                     IEFTRVPGQPNQWNAAVTVDPEAETATNTAAALGEAPEGTNNFIVEFTNLGTLRRVAD
                     GQGNQSPEEGTLAVNIAFDVQDATAEEGAGPVRQTFALNLGTAGSVRSTVTQFAEASS
                     TKVFEQDGYSMGYLETFKIDQSGIITAVYSNGSNRTIGQIALASFTNPGGLEKSGETN
                     FVQSNNSGLADIGPSGIAGKGKIIAGTLEMSNVDLAEQFTDMIVTQRGFQASSKTIQT
                     SDQMLQELLTLKR"
     gene            complement(11800..12234)
                     /gene="flgD"
                     /locus_tag="P5313_07620"
     CDS             complement(11800..12234)
                     /gene="flgD"
                     /locus_tag="P5313_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HRY80267.1"
                     /translation="MDFSAMMNPAELNRTQFDVDAFNKSLNGGKAFKPTLDKDDFLKI
                     LITQLQHQDPTKPMEDKEFIAQMAQFSSLEQMTNMGREFAKVASVLRVGEAQGMLGKN
                     VEIVNGSSSVTGTVTQVMRGDFPMVMVNGTFYDLAQVEKVIE"
     gene            complement(12307..13569)
                     /locus_tag="P5313_07625"
     CDS             complement(12307..13569)
                     /locus_tag="P5313_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRY80268.1"
                     /translation="MQAASVSVNPSSQVPQAERIRVPNPADPGGKAPHGASFLAILGR
                     SLRGMAGAEQDAAPRRNPRSGFPERPMTNRGQASGVAAGADRRAGRSGVSADNQVRAD
                     AVNAVRPGPDAAEAAMAVTPKDAKPAAAANRSGPEQLGPSPGKNNADAAELRTKSRER
                     KQDPASRTEDSAAAAAVPVPKPAARAAEARTPAEEPSVDGPGAGRRRGDRKDLKVSVV
                     DLRIRPENPEAEGGGKSGGTTEGPSTVRETVSAGARQAEAPVPADRPSNSKPAQGTGF
                     SDILARHIADAGAQDIVKAAHIVLRDGDSGLIRLRLEPESLGSVKIELKMTEKNITGR
                     IVVETDEARNAFEKSLSGLRDAFAEGGFETASLEVSVGGGQAEGGGDPADSGDGPFFT
                     ERLRDFDRSLPAAPDVLYGKDGRVNLWA"
     gene            complement(13630..14262)
                     /locus_tag="P5313_07630"
     CDS             complement(13630..14262)
                     /locus_tag="P5313_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HRY80269.1"
                     /translation="MPAFGKPRVLGRILVMLAIILALILGGLVWFDYLGLLDLKDTFA
                     PVYRLIGLPARRPGALPADSLTLLEDERNAKLYEALDAVREELDARSGSLDEKEAEIS
                     RIAQELEEQRKALEDREKSFNQTVESIENRKANVDQNARYLSGMPPKNAVEILKSMDD
                     QMVIDVLRAVERQAQEAGQDSVVAYWLSLLPPDRSAAIQRKMASKPEPLD"
     gene            complement(14280..14720)
                     /gene="fliJ"
                     /locus_tag="P5313_07635"
     CDS             complement(14280..14720)
                     /gene="fliJ"
                     /locus_tag="P5313_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRY80270.1"
                     /translation="MKRFRFRLEKVLEVRQYYERIAEMKLAAAAGKCALLERNLAENA
                     RATREAARQRFGAGRELPDMRTVELYALRLAAERERTLKALAFAEADREKARQGYVAA
                     SRETKLITKLRERGEAEYYRSAVREEVKTLDDLSRAGKNIGRDE"
     gene            complement(14717..16138)
                     /locus_tag="P5313_07640"
     CDS             complement(14717..16138)
                     /locus_tag="P5313_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HRY80271.1"
                     /translation="MENLSKYLSVVDRTDPIKYTGRVTKVQGLLVESHGPRAVIGEVC
                     KILIPKTDRAAFAEVVGLRGEAVQLMSYSNLDGIEAGCAVVATGDLLYVPVSKKLLGR
                     VLDSMGRPVDGKGDIVSDQFYPAVAQPPDPLTRRRITRRIVTGVRSVDGLLPLGKGQR
                     IGIFAGSGVGKSTILGMVARNTNADVNVIALIGERGREVREFLENDLGEEGLARSVVI
                     VSTGDTPPLARLRGAYVATAVAEYFRDQGLDVMLLFDSVTRFARAQREIGLAVGEPPT
                     TRGYTPSVFDSLPRLLERSGSSDKGTITGIYTILVDGDDMDEPISDTVRGILDGHIVL
                     ERRLAERYQYPAVGALKSISRLANAVTGPVTQKAMGVVRRLLATYEESEDMINIGAYV
                     KGTNPAIDEAIGKHEAIREFLVQGITEKAPITDTLRKLGDIAGVAIPASEISEYADSA
                     PSPVPKAVNTETASNQAEAGTAV"
     gene            complement(16149..17075)
                     /gene="fliH"
                     /locus_tag="P5313_07645"
     CDS             complement(16149..17075)
                     /gene="fliH"
                     /locus_tag="P5313_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HRY80272.1"
                     /translation="MAKTVFRPQEIVPLTSKYVLDGPDSGAAQEAGGAVEALPLDEYM
                     GPTADDLRREAEAFKANWDKERETMVASARAEADAIIQKAEAAAFDEVKRRTDQAQKL
                     KTRAEEEATAAVEAAEARIRELETQAKERIDSVHKDAFRKGFDEGRESGYKEGKAEVE
                     RLTGRLHAVIERALDKRGEILEKTEAEIVELVLLVAKKVVKVISENQKNVVVQNIVQA
                     LRKLRTRSEVTIRVNLADLQLASEHAKDFVAMAENAKHIQIVEDTTIDRGGCIIETDF
                     GEIDARISSQLHELEEKILDISPIKARGKPTA"
     gene            complement(17088..18095)
                     /gene="fliG"
                     /locus_tag="P5313_07650"
     CDS             complement(17088..18095)
                     /gene="fliG"
                     /locus_tag="P5313_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRY80273.1"
                     /translation="MKVKDMNGRQKAAIFLVTLGSEISAEIFKHLREDEIETLTFEIA
                     RLDTVEPEQKDQVLMEFQELMMASEFISSGGIDYARELLEKALGSQKAIDIINRLTSS
                     LQVRPFDFIRRTDPAHLLNFIQQEHPQTIALILAYLEPSKAGYILQKLHPDFQSDVAR
                     RIATMDRTSPETLREVERVLEKKLSTLSSEDFTAAGGVDSIVEILNLVDRTTEKGIIE
                     SLEEENPELAEEIKKKMFVFEDIVLLDDRSIQKVLREVDGQELAKALKGVDQEVQEKI
                     FKNMSKRAATMLKEDMEYMGPVRLKDVEEAQQKIVSIIRHLEETGDIVISRGEGDELV
                     Q"
BASE COUNT         2934 a         5889 c         5990 g         3491 t
ORIGIN      
        1 cgcgtagtcc gccagggtct ccaggatcac gaccatgttg cggaccgaca cctgctcccg
       61 aaggagggcc tggatgacct tctggatctc ccccagggag aggtgcttct gggcgtcctc
      121 caccaccgcg ggatagtcct tccggagtgt gtccaggata gactggatct cctgccgtcc
      181 caggatctcc gaggcgtgcc gtttgatgat ctccgtcagg tgggtggcca cgatgctggg
      241 ggcgtccacc acggtgtagc ccgcccgctc ggccttgtcc cgctggtcct cggctatcca
      301 gagggcggaa aggccgaagg ccggatcccg ggttttctct ccggggatct cctcggtcac
      361 cccgccggga ttgattgcca tgtagtagcc cagccggatg actccgcgcc cgacctcgac
      421 acccttgatc ttgaagcagt actcgctggg ctccaggcgc atgttgtcga tgatccggat
      481 gcggggcacc accaggccca tgtccagggc gctctccttg cggatccggg tgatgcgctc
      541 caggagttcc gcccccttgt cccggtccac cagggggatg aggccgtagc ccagctccag
      601 gctgatggga tccaggggta ccacgggcga gagctcctgg gcggactccc ccttggcctt
      661 ggcgcccgcg ggatgcgcct tggcggcttc cgcgtcccgg agctgcttgc gggaaaggcg
      721 gaacgcgagc caggccatgg aggccgccat gggaaggagg acgtaccagg ggaagccggg
      781 caggaaggcc agggcggcca ggaccgcgga cccgatccag tagatccggg cgttctggga
      841 gaactgggcc gtcacgtccc cgccgaaggt tccctgggag ttcgcccggg tcacgatgat
      901 gcccgtggcc acggacacca ggagggccgg cagctgggac agaagtccgt ccccgatggt
      961 cagggaggtg taggcctcga tggcggtctg gaagctctcg gagtggatca ccatgcccac
     1021 cacgaagcct cccagcatgt tgatgacgga gatgaagatc ccgaccttca cgttcccgga
     1081 cacgaacttg gaggcgccgt ccatctggcc gtagaaatcg acctcccgct ggacctcgtc
     1141 cttgcgcctc cgggcctcct cctccgtgat ggccccggag ttgaactcgc tttcgatggc
     1201 catctgcttg accggaagac cgtccagggt gaaccgggcc gccacctcgg cgatccgcgt
     1261 cgcccccttg gtgatgacga tggcctggac cgcgatgatc acgatgaaga tgatgaagcc
     1321 gatcaccagg ccctcggtgc ccccggagcc caccacgaag gtggaaaagg cgcggatcag
     1381 gcggccgtcg aagcggctgc cctgggtcag gatgagccgg gtggaggaga tgttcagggc
     1441 caggccgaag atcgtcgtca ccaggagcat ggtcgggaag atggagaact ccgtggcctt
     1501 ccgggtatac aggacgatca gcaggatcaa aaggctgatc gtgaggttga aggccatcag
     1561 ggcgtccagg agcaccgtgg ggatcgggat gatgatcatg aggaccaccg ccacggcccc
     1621 gatggccacg gcgaagtccg agcggttcgt caggaaggtg tccctcagga cccgttgggt
     1681 atcggacatc taagcctcct ccgggctcgg gcggcgcgtt cgtccggtca tgccagatcc
     1741 tccgccccgg cggcccgggc gtagcggcgg cccttgtcgg aaagggcgta gacgtgggcc
     1801 aggaccgcgg ctatcgcctg gtagtacttc tccggtatgg tctggccgat ctcggtttcc
     1861 gcgtacaggg cccgggccag gggccggttc tccaccaccg ggacccgggc gtcctcggct
     1921 atgcggcgga tgcggagggc cgcctcatcc atccccttgg cggtcaccat cggggcggcc
     1981 atggactcgc ggtcccattc cagggccacc gcgaagtggg tcgggttcgt gatgacgaca
     2041 tccgccttgg ggacgttgac cgccatgttg cgggtcagaa ggtcgcgcat ccgctcccgg
     2101 aggcggccct tcacctgggg atccccctcg tgcatcttac gctcttcctt gacctcctgc
     2161 ttggtcatct tcatccgctc cagaaactgc cgacgctgga acatgtagtc cggaatggac
     2221 agggccagga gggtgaccgc cacggccagg atgagcttca ggatcatccc ggagaccagc
     2281 ttgacggagg tccagaaggg ggccatgtag aggttcctga gacgctccag ctccccgttc
     2341 acgaggatcc aggccaccgt gctgatgatc gctatcttga ggatggactt cccgaggttg
     2401 tacaggccct ccatcgagaa gatcctctgg aagtactgcc cgagcctcgg gacgaccttc
     2461 ttgaagtccg gggtgatggg tttcacggag aagaggaacc cgacctgcag gatgttgccg
     2521 aaaagagccg ccaggactcc gaccgaggcc aggggcagaa ggagccggag gaggtaccgg
     2581 aagaagaggc ccgcggccag ggccccgtcg gtcacgatgt ccgattccgt ggacagcttg
     2641 aggaaccgta cgatcatctc cctcagggtc tggagcatcc agggggccag gatggccagg
     2701 gtcaccgcgg tcagcaggag gccgatggcc gccaccaggt cctggctctt ggctacccgc
     2761 ccctcttccc gggccttccg gagcttgtac tccgaaggct cctcggtgcg cccctcctcc
     2821 tccggggagg cgaaccactg gaggtggatg tccgccgggg agaggacccg gggaggcgcc
     2881 gggtaggctc ggaggtcggg ggccagggcc gtcatcccgt gccccccagc atccgggcca
     2941 ggtccaggaa gcccgcatcg atcagccggg cgaaggcttc catcaggaag ggaagcgagc
     3001 cgaagagcag gagaaaggcc accccgatgt tcaagggaaa gccctccgtc aggaggttca
     3061 tctggggagc cgccttggac aataggccca tggtcacgga gatgaccatc agggtgccca
     3121 ggatcgggaa ggccagggtc atggcgttct tgaacagcag ggacacggcc ccgaaaaggt
     3181 attcgaagaa cccgtcccgg cgcatcacca agtccaccgc ccgaagggcc tggaaggagc
     3241 cccggacccc gatcaggaag aggttgcgga aacccccgac ctgcacgaag accagcatgg
     3301 ccacgatgtt caggtactgt cccatgaggg gaatctcgat ctccgccagg gggtcgtaca
     3361 cttcggaggc cccgaagccc atctggaggg agaagaactg tcccgcagtc acgaaggcgg
     3421 cctggacggc gaccaggaaa aaccccgtga tgatccccag gagggcttcc ccggccagca
     3481 gggccaggta ggggccgatg gtatccggaa tggggtatcc ggcatcccgg acccagggga
     3541 agaccgcggc ggcggcgaga ccggacaggg cgatcttggc ggcctggggt atcccgtcac
     3601 tggacaggag gggagccgtc tcgaccatgg ccaggacccg caccgcgatg aggaagaaga
     3661 ggtccgcgcc gtccaggatc tcgttgaaca cgcccctacc tcgccgccat ggggatcagc
     3721 tcgaacaagg tccgggtata ctgggccagg tgggtaagca tccacccgcc caggatggcc
     3781 aggaccgcca ggatggaggc gatcttcgga acgaaggtca gggtctgctc ctggatcgaa
     3841 gtggtggcct ggaggatgga gacgatgagg cccaccacca gggccacgat gaggatgggg
     3901 gccgagatca ggacgatctc caggataccg ccccgcatca ggttgaccgc gtcacctatg
     3961 tccacatccg cctcctaccg gaacgaagcg acgagctgcc gggtcatgag gttccagccg
     4021 tccaccagga tgaagaggat cagcttgaac ggcatggata tcatgaccgg aggcagcatg
     4081 atcatgccca tggacatcag ggtggatgcc acgaccatgt caataattat aaacggtata
     4141 tataaatata tgccgatctt gaacgcaacg gtgagctcgt tcaggatgaa cgccgggatc
     4201 aggacgtagg tgggaacgtc cgccagggtg gtcggttttt cgaggccccg gagggccatg
     4261 aagaggcgga tgttctcggg attcgactcc atctgccggt acatgaagag cctaagggga
     4321 gcctctccgg ccaggtaggc ttcggatacg gtcatccgcc cctccgccat gggggtcagg
     4381 gcgtcccggt tgatctggtc cagggtgggc cacatgatga agatggtcat aaagaaggaa
     4441 atacccagga ggacctggtt cgggggcacc tgctggaggg agagggcccg tttgatgaag
     4501 tccaggacga tggagatgcg aaggaaactg gtcatcagga tgaggatgct gggcgccagg
     4561 ctcaggaccg taaggagcag gaggagctgg acggaaaagg ccacttcccg gttgttccgt
     4621 ggatttcgga tcgtcaggtc cacgaagggt accgcgggcg gcggggccgg aaaaccggtc
     4681 gtgccggcac ccgcccgagg cgtcgtcccg gcgcccggta ccgggacgct gaccgcggag
     4741 tcccgggggg cggtcgggaa gggggcggcg tcctgggccg tgacggccgg cagggatccc
     4801 gcaaagacca cggccgcaag gaccaagatg aggcggctct gcatctcccc tcccggtgcg
     4861 ccgggaacgg cttcagaatc tcttcagccg gtccctctgc cgcgccagga actccgtgcc
     4921 cggaatcccc ttcgcggaat cggcgcgatg cttcgatacg ggcttaagca agcccgaaag
     4981 gagggtgccg aaatcccggg tatcggcctg gggagcggtc gcggcccgga gcttcatctc
     5041 gtcgatgagt tccggatcct ccacctcgcg gatgaggttc aaggacgagt cggtcgcgcc
     5101 gatcaggaag gccttggctc ctagggaaac gacgtacagc caccttccgg tccccagggg
     5161 gaccgaggcg agaaccttga cgaaggcgtc ctcggcgatc cggggacggc cggcctttcg
     5221 aaggaaccgg aacgtcagcc agatggcccc caggacgaac gccagggtca acagcatccg
     5281 caggaaatac ggccaggcgg agtcggacag ggcgttccgg ttccccccgt tcgcggtttc
     5341 cgaggattct gccagtacga gatccgtttc cgcggcgggc gccgcggagc cttgtcccgc
     5401 ggacggcacg gtcccgcgta cgtccgtacc cggcgtcggc tgggcgaagg ccgacgggca
     5461 cagcacgcac aggaccgcca ccgcgcacag gacgcgatac ggtatgttca gctttcccct
     5521 ccgggatata ttctacatca cggaattcgg aaatgcaata gatattgacg ggaaagagaa
     5581 ataagcggaa tttcgaggtt tccgggggga gagggcggga cccggttcgg gatcccgccg
     5641 ccggccggat tctctagctc atgtcgctca tccgctccat aggagagacg atctccgtga
     5701 cccggacgcc gaagttttcg tcgattacca cgacctctcc cttggcgatg agcttgtggt
     5761 tcaccaggat gtcgaccggt tccccggcca gcttgtccag ctcgatgatg gtcccctcgc
     5821 ccatgcccag gatctccttg atgagcttgc gggtacggcc cagctccacg gtcatctcca
     5881 tgaagacgtc catgatgagg ccgatgttcc cggcctcggg cggggcgctc tgcatggcca
     5941 ggttggcgaa ctggacgggt tggacgttgg gcgggctcat cccccccatg gagttggcca
     6001 tgcccgcgaa cataccgccc ataggctggg aatacatccc catggcctgg gcgttcatgc
     6061 cgcccatgcc gcccatggcg ggagcctgca tggcgggctg ggcgggggcc ccgccgccgc
     6121 ccgcggcggc caccacgtcc cggacgaagg acagggagaa ggtttccacg aactgctcgg
     6181 gcttgcctcc gatgttgacc ggccatacca cccggatgaa gtccccggag ggcaggcgga
     6241 ccatcgcctt gggaacgtag ttcccctcgg cggaggagga ttggaggctg cggttgccgg
     6301 tgcggtccgt gaagaccgtg atcagggtcc ccgtgagggt ggacacgcat tcggagaccg
     6361 cggagatggc catgtcgtcc aggtccgtga gggaatcgtc cttgttggcc agggacgcga
     6421 tccgaaccgc ggtttccttg gaaaggatga acaggtgctc cccgggtatc ccgccggtca
     6481 tgtcgatctg gacggccagg acctcctccg gaacggactg caacaccgcg tcccgctgga
     6541 cctggtccac ctggggggcg ccgaccgtca cgtccagccc cgtgatcatg gacaggttct
     6601 gggactggga gcccgccgtg ccctcgagga tggacttgaa gaactgggct tcccgggaca
     6661 cgttcccccc gcccgaggaa ccgggagcgg cggccgtctt gcccccgccg ccgccagtat
     6721 ccaggccggc caggagggag tcgatctctt cctgagacaa ggcgccgtca ctcataggat
     6781 tctccttctt ccgatgtaag ttcctcgaac tcctcctgcc cgacgtcctc cagcttcttg
     6841 gtgatctgga cggccatttt ccggccgatc acgccggggc ggcagaggaa cttcttcttg
     6901 ttgcccacgt tcaggaccat gggatcgtcc accttcacgt tgtagagccg gaccacgtcc
     6961 ccggcccgca gggacaggac gtcccggacg gaaacctgga tcttgccgat ctccgcgatg
     7021 acggggatct cgacggtgga cagcttctcc ttcaggatgt tcaggttctc cgtcgtcgcg
     7081 ccccggcgca cggagctgta ccagtattgg gcggagagct tggagacgat gggttcgatc
     7141 gtcaggtaag ggatgcagag gttcatcatc ccctctatct cccccacctt ggtctccagg
     7201 gtcacgagga ccaccatctc cgtggggggg acgatctggg cgaactgggg gttcgtctcg
     7261 atctgggaga gccggggacg caggtcgatg acctgggtcc aggcctcccg catgttgccc
     7321 agtatccgga cgatgatgcc ctccatgacg gagtgctcga tgtccgtgag ttcccggttg
     7381 agcttggatc cctcccccga tccgccgaac aggcggtcga tgatggtgaa ggtcacggag
     7441 gggtcgatct ccaggatggc ctggcccttc aggggatcca tgctgaccac ggccagggtg
     7501 gtcggcgtgg ggatggagcg gatgaattcc tcgtaggtca gctggtccac cgaggccacg
     7561 tggacgtgga ccagggtgcg cagggaggcc gacaggcttg tggtggtgag acgggcgaag
     7621 gtctcgtgca tgatctggac ggtgcggatc tgttccttgg agaacttgtc cggccgcttg
     7681 aagtcgtaga tcttgatctt gcgggaatcc gtggtcggcc ggatggcctc cacgtccgtt
     7741 tcccccgcgt tgatcgccgt gaggagctgg tctatctcat cctgggacag gacttccgtc
     7801 atgcctcccc ctcccgcgtc ccatcattgc acgaattcta ccggcgttca aggccggttt
     7861 tacatcttga tcacgtccag cttctcgaac aggacttcct tgatggaagg cttgctgagc
     7921 atgttgttga ggtcttcccg gatctcttcc ttgagccgct gttcgttctt cggggcgagg
     7981 tagtccgcgg tcttggagct gaaatagttg cggagcctgt cccgcatctg gtggatccgg
     8041 gcggtcagca ttgtcggggt gtccttgtcc cccttttcga agcccaggtt gatcttcacg
     8101 acgatggagg acggctcgtt gtctatggtc cgggtccgga tctccccgag ggtggtgaag
     8161 tactcgtact cggggctggc cctctggtag ctttccccca gggccacctg ggtcatgggc
     8221 ttgccctgct tgttgacgat gttgaccgtg acgatcacca cggtcaccac gaagatgacg
     8281 gcgcccaggg cgatgccgat ccacatgagc aggcggagga tgccggcgcc gccccccttg
     8341 gcccgaacgg gagccggggc gcttcccccg tcttcgttga ggttcatgcc ctgttcttcg
     8401 aagctgtcgc ccattccgta cctccgcatt gcgacggggc ggcccgtttt ccgggtcccg
     8461 ggcggccccg cgtccccttg tcgggaacat cacctatgta atgatcggcg aaaagattgt
     8521 ccggctttcg cggaaattga gtccggctac aggtgggcct cgtccaggat gatgatgtcc
     8581 acccgccggt tgtaggcccg gccctcctcg gtgtcgttgg aggcgatggg caccgtgtcg
     8641 gcgaatccgg ccacctggaa gcggcgttcg tccgccccga agtccgtcag gtagtgcagg
     8701 acgttgaggg accgcgccgc ggagagctcc cagttggacc gccagggtcc ggcggggtcc
     8761 gtgctggtgg cgtccgtatg tccctcgatg cggaatttgc ggcctcccag ctcgtcgctc
     8821 ttcaggagtt ccgccaaacg caccagcatc gttcgggttt cctcgatgtt cacgtccgcg
     8881 ctggccggac ggaagaaggc gtcggaggcc agggagatca cgatcccccg ctcgtccgtg
     8941 gagatcctca ccttgttgga tttgatctcg ggctggaata gactggtggc cttcttcttg
     9001 gccgtggcca ggagccggcc cttctccatc gagggcaggg ttgataccgt gtttcccagt
     9061 tccgcgagct tgcccaccga gagggtgttg ccccccgtgg aggctcccat gccgatgttg
     9121 ttcatcgagg agatgagcac ctgcatctgg gtgatgtcca cgtcggtcac gtcgaagagg
     9181 gccacgaaga agcagaggag cagggtcacc atgtcggagt aggtcacgat ccactccccg
     9241 gtgccccctc cgatctcctt ccgctgtttc ttggccatgt ctagtccttg ccgtacttgt
     9301 cgatgagttc cttcttgtcc gcgggctcca ggtaggaggt gagcttgaca aggaggatgc
     9361 ggggattgtc gccggcctgt atggacagga cgccctcgat gatcatctcc cggagccggg
     9421 tctccatggc gtccttggcc gcgagcttgc ccatcatggg agtcaggagg aagttggcca
     9481 tgatggcccc gtagtaggtg gtgatcaggg ccacggccat gttcgggccg atgcggctct
     9541 tgtcctccag gttcttcatc atcgctatga ggccctggac ggtgcccagc atcccgaatc
     9601 ccggggccag cttggaccag ttgtcgatca tcttgagcca ccgggcgtgg cgctcgttca
     9661 tcgcgttgag ctcgttttcc atgaggttcc ggatgatctc ggcgtccgtc ccgtccacga
     9721 caagccggag accggactgc atgaacttgt cgtccagctc ggcgatcact tcctccaggg
     9781 ataggatgcc ctcgcgtcgg gccttctcgg ccagttcgta gagcttctcg acgatcttgg
     9841 attccccgaa ctcctccacc cggaagcact tgcccaggat ggaaaagagc ccgatcgact
     9901 ccttgaggga gtagttgagc aggagggcgc aataggaccc gccgatggtc atgaatacgg
     9961 acggaatgtc gatataggtc ccgacctgtc cgcccgaagt gtacatggcc aggatgatgc
    10021 aggctatacc gccgattagg ccgatcaggg aaccaagatc catactcact cctcgttctt
    10081 gaaggccccg atctgccgcc ggtaggcgac gatccggccc agaacctcgg gcacgccctc
    10141 cagcacgacg atccgcttcc cggacagcat gatcagcgtc gtgtcgggat tcttctcaat
    10201 atattcgatc tggtgcgggt ttatccagaa ggtcttgccg gaaagctggg tgagttcgat
    10261 catggcgtgg acagtggacg gatgacggcg gacagaaatg gcttcggtgc gcgactaacg
    10321 actacgcttc gacgcaggtg gctgccgcgg ccggggatca tttccgttta ccgttcaccg
    10381 tccaccgttt acctcctacc gtttcagcgt cagaagctcc tggagcatct ggtcgctggt
    10441 ctgtatggtc ttggaactgg cctggaagcc gcgctgggtg acgatcatgt ccgtgaactg
    10501 ctcggccagg tccacgttcg acatctccag ggtgcccgcg atgatcttgc cctttcccgc
    10561 gatgccggag ggccctatat ccgcgaggcc ggagttgttg gactgcacga agttcgtctc
    10621 cccggacttc tccaggcccc cggggttggt gaagctggcc agggcgatct gcccgatggt
    10681 ccggttcgat ccgttggagt agaccgccgt gatgatcccg gactggtcga tcttgaaggt
    10741 ctccaggtag cccatggagt atccgtcctg ctcgaagacc ttggtgctgg aggcctcggc
    10801 gaactgggtc accgtgctcc ggacgctgcc cgcggtcccc aggttcaggg cgaaggtctg
    10861 ccgcacgggg ccggccccct cttccgccgt ggcgtcctgg acgtcgaagg ctatgttcac
    10921 cgccagggtc ccctcctcgg ggctctggtt gccctggccg tccgccaccc ggcgcagggt
    10981 gccgaggttg gtgaactcca cgatgaagtt gttcgttccc tccggggcct ccccgagggc
    11041 ggccgcggtg ttggtggccg tctcggcctc gggatccacg gtgaccgcgg cgttccactg
    11101 gttcggctgg ccgggcaccc gggtaaactc gatgcgcagg atgtgctcga tcccgaagga
    11161 gtcgtagatc ttctcctcca cccgccaggt cccccggagg atcgtctcct ccgaggcccc
    11221 ttcggggatc tcggggacgc gcttgtccag gttgcaggcg aggtagacct gctcggtggc
    11281 cttggcggga tccttggatc cgacggggat gaccaggtcc tccagggcgc cggaggtgtt
    11341 caggatctcc cggccgttca cgacctgggc gttccagccc tggacccggt acccgttcgc
    11401 ggggttcacc aggcgcccgt cccggtctag gccgaaggcc ccggcccggg tgaagaaacg
    11461 ggagtccccc ttctggagca cgaagaaacc gttcccctgg atggcgatgt ccgtcatgac
    11521 ccccgtggtc tggagggacc cctgggtgtg gatcgtgtcc accgaggcga tcgacatccc
    11581 gaggccgatc tccttcgggt tgacaccgcc cacctcctcg ttcggacgtg cggctccgga
    11641 catctgctgg tacaggaggt cctggaaatt cacacggttc ttcttgtatc ccgtggtgtt
    11701 gacgttggct atgttgtgtc cgagcacgtc catccggacc tggtggttct gcaggcccga
    11761 gacgccggaa tacagggatc gcatcatgga gaggcctcct tactcgatga ccttctcgac
    11821 ctgagcgagg tcgtagaagg ttccgttcac cattaccatg gggaaatccc cccgcatcac
    11881 ctgcgtgacc gtcccggtga ccgaggagga cccgttcacg atttccacgt tcttccccag
    11941 catgccctgg gcttccccga cccggagcac cgacgcgacc ttggcgaatt cccgccccat
    12001 gttggtcatc tgctccaggg aggagaactg ggccatctgg gcgatgaatt ccttatcctc
    12061 catgggtttg gtcgggtcct gatgctgcag ctgggtgatc aggatcttca gaaaatcatc
    12121 cttgtccagg gtgggcttga aggccttgcc gccgttcagg gacttgttga aggcgtccac
    12181 atcgaactgg gtcctgttca attcggcggg attcatcatc gcggaaaaat ccattctatc
    12241 ggcctccata tgctacgact cgcaaaacct gccgatcggc cgcattgacc ctcggctccc
    12301 gatccgtcag gcccacaggt tcacccttcc gtccttaccg tacaggacgt cgggcgccgc
    12361 cggcagggat cgatcgaaat cccggagcct ctccgtgaag aaggggccgt cgccggaatc
    12421 cgcgggatcc ccgccaccct cggcctggcc gcctcccacg gaaacctcca ggctggcggt
    12481 ctcgaagccg ccttccgcga aggcgtcccg caagccggaa aggctcttct cgaacgcgtt
    12541 ccgggcctcg tccgtttcga cgacgatccg gcccgtgata ttcttttcgg tcattttcag
    12601 ctctatcttg acgctcccca gggattccgg ttccaggcgg agacggatca atccggaatc
    12661 cccgtcccga aggacaatgt gggcggcctt tacgatgtcc tgggctcccg cgtccgcgat
    12721 gtgccttgcc aggatgtccg agaagccggt gccctgggcg ggtttggagt tcgaagggcg
    12781 gtccgccgga accggggctt ccgcctgccg ggcccccgcc gagacggttt cccggaccgt
    12841 ggacggcccc tccgtggttc cgccggactt cccgccccct tcggcctccg gattctccgg
    12901 gcgaatccgg aggtccacca cggagacttt caagtccttc cggtcgccgc ggcgacggcc
    12961 cgctccgggt ccgtccacgg acggttcctc cgcgggcgtc cgcgcctcgg cggcacgggc
    13021 cgcgggtttg gggacgggca ccgccgcggc cgcggcggag tcttccgtcc gggatgccgg
    13081 gtcctgcttc cgttccctgg atttcgtgcg gagttccgcc gcgtccgcat tgttctttcc
    13141 gggtgagggg ccgagctgtt ccgggccgga tcggttcgcc gcggcagccg gctttgcatc
    13201 cttcggcgtc acggccatgg ccgcctcggc ggcgtcgggg cccgggcgga cggcgttcac
    13261 ggcatcggcc cggacttgat tgtccgcgga tactccggac cgcccggccc ttcggtcggc
    13321 tcctgcggcg acgccggagg cctggccgcg gttcgtcatc ggccgttcgg ggaatcccga
    13381 tctcggattc ctccgcgggg ccgcgtcctg ttccgccccg gccatgccgc gcaggctccg
    13441 gcccaggatc gcaaggaacg acgccccgtg cggggccttg ccgcccggat ccgccggatt
    13501 cggaactcga atccgttccg cctgggggac ctgggaggag ggattgacac tgacagaggc
    13561 tgcctgcata ggcgcggcgc tctcctttcg ttccgtcggc cggaaccgga gccgccgcgc
    13621 tgcggaagat cagtcgagcg gttcgggctt cgacgccatc ttccgttgga tagctgcgga
    13681 tcggtccggg ggcaggaggg acagccagta ggccaccacc gaatcctgcc ccgcttcctg
    13741 ggcctgccgt tccaccgccc ggagcacgtc gatgaccatc tgatcgtcca tggacttcag
    13801 gatctcgacg gcgttcttgg gcggcatccc cgacaggtac cgggcgttct ggtcgacgtt
    13861 cgcctttcta ttctcgatag actcgacggt ttggttgaac gatttttctc gatcttccag
    13921 ggctttccgc tgttcttcca gttcctgggc gatccgggag atctccgcct ccttctcgtc
    13981 cagggaaccg gaccgggcgt cgagctcctc ccgaaccgcg tccagggcct cgtagagctt
    14041 ggcgttccgc tcgtcctcca ggagggtcaa ggaatccgcg ggcagggccc cgggcctccg
    14101 cgcgggcagg ccgataaggc gatagaccgg ggcgaaggtg tccttgaggt ccaggagtcc
    14161 caggtaatcg aaccagacga gacctcccag gatcagggcc aggatgatcg ccaacatcac
    14221 gagtatgcgt cccaggacgc ggggtttccc gaaagcaggc atcgttctac ctctatacct
    14281 tactcatctc gtccgatatt cttgccggcc cgggaaaggt catccagggt cttcacctct
    14341 tcccggacgg cgctgcgata gtactccgct tcgcctctct cccgcaactt ggttatgagc
    14401 ttggtttccc ggctggccgc cacgtaaccc tggcgggcct tctcgcgatc cgcctccgcg
    14461 aacgccagag ccttgagggt gcgttcccgt tcggccgcga gacggagggc gtacagctcc
    14521 acggtccgca tgtcgggcag ttcccggccg gcgccgaacc tctggcgcgc cgcctcccgg
    14581 gtggcccggg cgttctccgc gagattccgt tccaggaggg cgcacttccc cgccgcggcc
    14641 gccagtttca tctccgcgat ccgctcgtag tactgccgca cctccaggac cttttccagc
    14701 cggaaccgga atcgcttcat accgcggtcc cggcctcggc ctggttcgag gcggtttccg
    14761 tattcacggc cttcgggacc ggggacggtg cggaatcagc gtactcggag atctccgagg
    14821 cgggtatggc cactccggcg atatccccca gcttccgcag ggtgtccgtg atgggggcct
    14881 tctccgtgat accctgaacg aggaattccc ggatggcctc gtgcttgccg atggcctcgt
    14941 cgatggcggg gttcgtgcct ttcacgtagg ccccgatgtt gatcatgtcc tcggattcct
    15001 cgtaggtcgc cagaagcctc cggaccaccc ccatggcctt ctgggtcacg gggcccgtga
    15061 ctgcgttggc caggcgggag atggacttga gggcccccac cgcggggtac tggtaccgtt
    15121 ccgccagcct ccgctccagg acgatgtgtc cgtccaggat gccccggacc gtgtcggaga
    15181 tgggctcgtc catgtcgtcg ccgtccacca ggatcgtgta gatccccgtg atcgtgccct
    15241 tgtcggagga gccggaccgt tccaggagcc gcggcaggga gtcgaagacg ctgggggtat
    15301 agccccgggt cgtggggggc tccccgacgg ccagcccgat ctcccgctgg gcccgggcga
    15361 accgcgtcac cgagtcgaag aggagcatga cgtccagccc ctggtcccgg aagtactccg
    15421 cgacggcggt cgcgacgtag gccccccgca ggcgcgcaag gggcggggtg tccccggtgg
    15481 aaacgatcac cacggaccgg gccagtcctt cctcccccag gtcgttctcg aggaattccc
    15541 ggacttcccg gccgcgctcc ccgatcaggg cgatgacgtt cacgtccgcg ttggtgttcc
    15601 gggcgaccat gcccaggatg gtggactttc cgactccgga gcccgcgaag atcccgatcc
    15661 gctgcccctt gcccaggggc agaaggccgt ccacggagcg gaccccggtc acgatgcgcc
    15721 gggtgatccg cctgcgggtc aggggatccg ggggctgggc gacggcggga tagaactggt
    15781 cggagacgat gtcccccttg ccgtccacgg gccttcccat ggaatcgagc acccgcccca
    15841 ggagtttctt ggagacgggc acgtacagga ggtcccccgt ggcgaccacc gcgcagcccg
    15901 cctcgatgcc gtccaggttc gaataggaca tgagctggac ggcctcgccc cgaaggccca
    15961 ccacctccgc gaaggcggcc cggtccgtct tggggatgag gatcttgcag acctccccga
    16021 tcacggcccg gggaccgtgg gactccacca ggagtccctg gaccttggtc acccggcccg
    16081 tgtacttgat cggatccgtc cgatccacga cggaaaggta cttcgaaagg ttttccatga
    16141 tccccgccct aggccgtcgg cttcccgcgg gccttgatcg gggagatgtc caggatcttc
    16201 tcctccaact cgtgcagctg gctggagatg cgcgcgtcga tctccccgaa gtccgtctct
    16261 atgatgcagc cgccccggtc gatggtcgtg tcctccacga tctggatgtg cttggcgttc
    16321 tcggccatgg ccacgaagtc cttggcgtgc tcggacgcga gctggaggtc cgccaggttc
    16381 acccggatcg tgacttcgct ccgggtccgg agcttgcgca gggcctggac gatgttctgg
    16441 acgaccacgt tcttctggtt ctccgagatg accttgacga ccttcttggc caccaggagc
    16501 accagctcca cgatctcggc ctcggtcttc tccaggatct ccccgcgctt gtccagggcc
    16561 cgctcgatga cggcgtgcag gcggccggtg aggcgttcca cctccgcctt cccttccttg
    16621 tagccggatt cccgtccctc gtcgaagccc ttgcggaacg cgtccttgtg gacggaatcg
    16681 atgcgctcct tggcctgggt ttccagctcc cggatccggg cttccgcggc ctccacggcc
    16741 gcggtggcct cctcctccgc cctggtcttc agcttctggg cctggtccgt ccggcgcttc
    16801 acctcgtcga aggcggcggc ctccgccttc tgtatgatcg cgtccgcctc cgcccgggcg
    16861 gaggcgacca tcgtctcccg ctccttgtcc cagttcgcct tgaacgcctc ggcctcccgg
    16921 cgcagatcgt ccgcggtggg acccatgtac tcatccaggg gcagggcctc cacggcgccc
    16981 cccgcttcct gggcggcgcc cgaatccggg ccgtccagca cgtacttgga ggtgaggggc
    17041 acgatctcct ggggacggaa gacggttttc gccatggtcg accttcctta ctgcaccagc
    17101 tcgtcgccct ctccgcggga gatgacgatg tcgccggttt cctcgaggtg gcggatgatg
    17161 gatacgatct tctgctgggc ttcctccacg tccttgagcc ggacgggacc catgtactcc
    17221 atgtcctcct tgagcatcgt cgcggcccgc ttggacatgt tcttgaagat cttctcctgg
    17281 acctcctggt ccacgccctt gagggccttg gccagttcct gtccgtccac ctcgcgcagg
    17341 accttctgta tggaccggtc gtccagaagg acgatgtcct cgaacacgaa catcttcttc
    17401 ttgatctctt ccgcgagctc ggggttctcc tcctccaggc tctcgatgat ccccttctcc
    17461 gtggtgcggt ccaccaggtt caggatctcg acgatcgagt ccacgccgcc ggcggcggtg
    17521 aaatcctcgc tggacagggt ggagagcttc ttctccagca cgcgctccac ctcgcggagg
    17581 gtctccgggc tcgtgcggtc catggtggcg atgcgccggg ccacgtcgga ctggaaatcc
    17641 ggatgcagtt tctggaggat gtaccccgcc ttgctgggct ccaggtacgc caggatcagg
    17701 gctatggtct ggggatgctc ctgctggatg aagttcagga ggtgggcagg atccgtcctg
    17761 cggatgaaat cgaagggccg cacctgcagg gagctggtca gccggttgat gatgtcgatg
    17821 gccttctggg agcccagggc cttttccaga agttcccggg cgtagtcgat gccgcccgag
    17881 gagatgaact ccgaggccat catgagctcc tggaactcca tgaggacctg gtccttctgc
    17941 tccggttcca ccgtatccag ccgggcgatc tcgaaggtca gggtctcgat ctcgtcctcc
    18001 cgcaggtgct tgaagatctc cgcggagatc tcgctgccca gggtgaccag gaagatcgcg
    18061 gccttctgcc ggccgttcat gtccttgacc ttcatcgcct tcttggccgg ggcgggcgcc
    18121 cctcccgcgg cgggtccgga ctgccctccc ctgccgcgag gctggggctg cgcttgagct
    18181 tgggcttggg cggccatggc ttactcctcc ctcagccagg tccggatgag ctgggccacg
    18241 tcctcggggt gctccttggc catgttgatc gcgtgctcct ggagctccag gcgcttgcgc
    18301 tcct
//
LOCUS       DAOTEI010000060        18147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2072_length_18147_cov_5.674884, whole genome shotgun sequence.
ACCESSION   DAOTEI010000060 DAOTEI010000000
VERSION     DAOTEI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 18147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18147
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2072_length_18147_cov_5.674884"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="P5313_07655"
     CDS             <1..372
                     /locus_tag="P5313_07655"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRY80274.1"
                     /translation="RELLEAEQYGLELLQKLPGDRNVKLWPPLVRSTLEKAGKTIEDL
                     DHVIFTQINRSVIEEVMGILGLPMERTTCVMDRYGYTGSGCVPMALHHAVKEGRIRRG
                     DLVCLVASGAGFAVGSNLFVY"
     gene            434..1744
                     /locus_tag="P5313_07660"
     CDS             434..1744
                     /locus_tag="P5313_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRY80275.1"
                     /translation="MSDWKEEYRKRLVSIDEAIRVIKSGDDVVVAQCASEPQGTMSRL
                     HTIADRVTDVRVFSVLTLKPYDFYAKPEMKGHFELASWFHAPGSREALKRGTGTVTFV
                     PNMLHRAATDRIHARRPDVFIGTCTPPDRNGFVSLSLGITYEKDILEAASAVILEVNP
                     RLPRTLGDTHLRVTDATYFVEHDQEVPALPAPQPTETDLSIGGYIADLVEDGSTIQLG
                     IGGIPNAAALALRGKKDLGVHTEMMVDSMMELYEMGVITNKRKSLFKDKFVCTFAMGS
                     RALYDWLNDNVAVEFRRGRWVNDPAVVAQNSRMVSINTCLMVDFTGQVASESLGTQQY
                     SGTGGQSDTAQGAVAGFDGLGKSIIACYATAKGGAVSTIVPTLPEGTAVTLHRSFVDH
                     IVTEHGIARLRGRTVRERTGELIAVADPRFREELTAQARKLGYL"
     gene            1928..2686
                     /locus_tag="P5313_07665"
     CDS             1928..2686
                     /locus_tag="P5313_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRY80276.1"
                     /translation="MLRDAHIHAPELAERDPGFPERFRAGGYRACSAAHSGPELDAAR
                     ALRDSGLDLRLSFGIHPQWTIWTWADRLAEEARDGRIEAIGEAGFDFFGDRPERVRNP
                     ENLAAQRSAFEYQLGLAERYGLPLVLHVRRALDLVFEYAPRLKRIPAALLHSWSGPLA
                     EARSLLAKGVPAYFSFGAPVLNGNRKARESCALLPADRVLLETDAPWQPPRGAEFCTL
                     EHLALVRDEAARLRGISPEAMEESAARAFETVFG"
     gene            2913..3494
                     /locus_tag="P5313_07670"
     CDS             2913..3494
                     /locus_tag="P5313_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80277.1"
                     /translation="MKRAYRLAALSLAFAALLTPVGAQESDEGFLARISAGLDVIDIG
                     LAAGVGAVYRFPGPGGIGEIAADIFYGPYWETYTEGANTFDYSETLIIVAVRADWLFN
                     YEAGVPGWYQVAGTGVFAGSYSWENYNRTTDYTEGNGYFASGTVINLGLGYVFSRTWE
                     ARLEIPVLVFFGEYGEAAAIAIPITAGVLFRPR"
     gene            complement(3538..4038)
                     /locus_tag="P5313_07675"
     CDS             complement(3538..4038)
                     /locus_tag="P5313_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRY80278.1"
                     /translation="MNFALMVIDLQKAYYRGAAKESMDTACEYINAVLPLFRKRGSPV
                     LWVQHMDEEDGVVPGKEGFELLDALKPEPAEPRIRKAYGNSFNRTDCARILAESRPDL
                     VVMTGYCAEHCVLSTYRGAQDLDLLPVILRNGIASGSRENKAFVEGISNVITYGVLRK
                     LLESAS"
     gene            4160..4915
                     /locus_tag="P5313_07680"
     CDS             4160..4915
                     /locus_tag="P5313_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRY80279.1"
                     /translation="MPFTDRTRILLGDAAVDRLARARLCVFGLGGVGSACALDLVRAG
                     IGNLEVVDFDVLQESNLNRVTYAYRENLGRPKAEVFREAALRINPEVRVEILASLVRG
                     AEAADRILEGCDFYLDAIDTLNPKTNLIAALAAREAPFASCLGTAGRIAPERLRLSSI
                     WESRGCPLGQKLRQRLRRRGVTRDFPAVWSDEPPVPPALPEDGSRPGDVADGIRIRAV
                     QGSGPFVPQAAGHILASVAVRAILGLPFGEPRV"
     gene            complement(4985..6214)
                     /locus_tag="P5313_07685"
     CDS             complement(4985..6214)
                     /locus_tag="P5313_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRY80280.1"
                     /translation="MSRLKDENLHESGRRKIEWVKPRMPVLSGLAKELAGTFRGVSVA
                     MSIHLEAKTAYLAEVLTGLGAKVAITGSNPLSTQDDVAAALARTGAEVFAWHGISPKD
                     QEACIAEVLAINPDLVLDDGADLTVTLARRGGASRVRAITEETTTGLQRLRALAREGK
                     LPAPVLAVNDAQSKSLFDNRYGTGQSSWDGIMRTTNLTVCGKTVVVAGFGWCGRGLAL
                     RARGLGANVVVTEVDPIKAMEAHFDGFQVLPMEKAAVLGDIFVTATGNTNIVDTRHFG
                     LLKEGCILCNAGHFDVEVQMRELTSYAKGFREVRPNVREYELPTGRRVYVLGEGRLVN
                     LVCGDGHPAEIMDLSFSLQLLCLAYGLKNGAGLAKTVHPVPGEVDRRVAALKLAAAGI
                     TIDVLTKEQEAYLASWD"
     gene            complement(6251..7417)
                     /locus_tag="P5313_07690"
     CDS             complement(6251..7417)
                     /locus_tag="P5313_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY80281.1"
                     /translation="MGDTENTGRASEPAVRKAARVLMFGVAVALALGLGGWALLRSRS
                     PGVPMPVLGVDGRPVPGSISEKIFVDIGGVRQGMFIRSADPANPVLLFVHGGPGMPEY
                     FLDELYPTGLESRFTVCWWEQRGAGLSYSPGMDPGTLTVDRLVSDVLEATDYLRSRFG
                     KDKIYLLGHSWGSFLAIKAADRAPEKFLAYIGVAQVADQTESEKRAYAYMLDRYMEAG
                     NEKKLRKLWKYPILESGEDVLLSYMGSLLRDETMHELGIGTTRTMKSVITGVFLPVWR
                     SRAYTLREKADIWKAKAFLARSTDLRSRLLTARPEEEILSLDIPAYFLSGRHDLTVSR
                     DLSRRYLGKLAAPVKGFYTLEDSAHSPMFEEPKKFLRILTEDVLAGTVNLADRD"
     gene            complement(7508..8107)
                     /locus_tag="P5313_07695"
     CDS             complement(7508..8107)
                     /locus_tag="P5313_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRY80282.1"
                     /translation="MSGNSYEKPDHWTLKAKSEGYPARSVYKLAEMQEKFKLLKPGTR
                     VLDIGAAPGSWSLWVLRFLKGSGFLAAVDLSPLGMKPPFANFFFLQGDLYDPAVRAAL
                     AERGPYGLILSDAAPSTTGDRTVDTGRSEALVEAVIGYADALLAPGGHLVAKLFQGGG
                     ERDLLQALRGRFETARGFKPKACRAESFETYLLGLGKKS"
     gene            8185..9222
                     /locus_tag="P5313_07700"
     CDS             8185..9222
                     /locus_tag="P5313_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY80283.1"
                     /translation="MRPEYTEILAKIEAELTRELPETPADGWLESRFGPLASLPNEAD
                     RQAVTAPGRELVLRGGKRWRPLLQVLCARAFGSGDLALALAPLPEIVHNGTLIVDDIE
                     DGAEVRRGGPAVHLAHGLDAAVNGGNLMYFLPLALLEDRVPDPVLRARLYGAYAAQLR
                     RVHLGQALDIAWHRDPGRLPSEPEYLLMCSLKTGALSGLAARIGALCGGAPDAAADAL
                     VRAFEEAGVAFQILDDVRNLRTGNPGKERGDDIVEGKKSLPVLRACARDPSLAGRLPG
                     FFGRAKAGGVRDPAVEEAVAAISATGALEEAEAEAAGLLARAEAAAGTACPPGPGRES
                     LLGIFPLLAGK"
     gene            complement(9257..10468)
                     /locus_tag="P5313_07705"
     CDS             complement(9257..10468)
                     /locus_tag="P5313_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRY80284.1"
                     /translation="MTSYKSDGQWWVSPYNFKPEVLAGRERPGKVRIHDATLRDGEQT
                     PGVVFRKEHKVAIAKALDAVGVDRIEAGMPAVSEEDFRAVEEIAALGLKAKIFSFART
                     MTEDIDKAVECGAHGVVIEIPIGYPKLVHQFKWTWEDVLRKSVPVINYAKSKGLYTVY
                     FPYDATRAREEDLDALLPAIMRESPPDSVGLVDTMGCASPEAIAYLTRKYRDITGLPV
                     EIHTHNDFGLAVATELAAVGAGAEVVHSCVNGLGERTGNAALEELILALDLLYGEGTG
                     CDPAALLGLSALVQEISGVRLAQNKPVVGEENYTRESGIGVNLVVEEPLAMFATHPAL
                     TGRKGKIVLGKKSGKPSVTYKLAELGLGELDEETAAAVLAEVKRLGNERRGLVGDEDF
                     RAIVARARGGK"
     gene            complement(10496..11227)
                     /locus_tag="P5313_07710"
     CDS             complement(10496..11227)
                     /locus_tag="P5313_07710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRY80285.1"
                     /translation="MVEPIKKTSVVEQAIGRIYELIKSENLRERDRLPPERRLSEMLG
                     ISRNSLREAIRVLDMLGILRVDQGSGMVIDSSRVSDSVTRHLSFALLLNRDRLQELFE
                     ARLVIETECAGLAAERATAEEVEELRTALSELEASGADRERGIRCELRLHDLVARASR
                     NTVLGRILESLRDILRESRETTVPASGVTARTIESQRRIVEAVGNRDAAAARDLMREH
                     IAAVAERIQKAASASAGRDSTGASA"
     gene            11335..11889
                     /locus_tag="P5313_07715"
     CDS             11335..11889
                     /locus_tag="P5313_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRY80286.1"
                     /translation="MATNTLVEADIWTVAQTDQGNVVLVRPKRSELAVPIFIGQLETQ
                     SILIGLGRVEVPRPLTHDLFLSTLKALGADLTRIEINDLREGTFYARLILARGEETLV
                     VDARPSDAIAAAVRAGCPVFIAEAIVEEAGISVNLVSESSKLEAAGSEDEKERLRKEL
                     EEAIGEEDYERAARIRDKLKELEG"
     gene            11985..12896
                     /locus_tag="P5313_07720"
     CDS             11985..12896
                     /locus_tag="P5313_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRY80287.1"
                     /translation="MLKSRRRDGTLLRDLPAFTRIMPYLMPDKAGSVIFYQEDYDVTE
                     CLAYIKDWNRRNARTAGALLTLFEVVLVAAARAVALRPRINRFIANRRYYQRNQILLS
                     FVAKKELSDDGKEVNVKVPFDPEDTLGSAARKVRDYIKKGISEDGHENEKVVDSVMRL
                     PSFLLKFINFAYNRLDDHNLLPYSVTESDPMWSTIFLANVGSFGLDAPFHHLFERGTC
                     PIFMAVGKVRDENRVSEAPDGTPRVETRKILRVNYSFDDRIADGIYMGRSLELVRNFV
                     EDPAALDRPPEIPPEIRAELALRPAGD"
     gene            12961..13938
                     /locus_tag="P5313_07725"
     CDS             12961..13938
                     /locus_tag="P5313_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80288.1"
                     /translation="MKATSSFRPFPALLLALAASAAWAQGAASSPGTPLPAAADPPRA
                     VALIAKYCSACHGWASGYKEIAAEGVIVPGKPSESRLFEYVESGYMPPSPPAPSPAEI
                     ELLRAWIASGAAAPEAVSGATESADAGSAASAGAPGKGGGFLGFKSKVDYHRAAGWTS
                     SGLLLAAGVVGAVRAYDLMDAAHDYRDAMGITEDTMDPVCYDMIRDLWSGDQALRWTH
                     VSLLAAGETLYLGNAITGMSMARKAKPGQVSKSDIHRWAFFTHAALMASEIVMGLFTT
                     DALKRGDHETVANLGRAHAAVGLAIPLVMIGAGIVIELPARPKTAPPAE"
     gene            complement(14165..16042)
                     /gene="htpG"
                     /locus_tag="P5313_07730"
     CDS             complement(14165..16042)
                     /gene="htpG"
                     /locus_tag="P5313_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRY80289.1"
                     /translation="MAQKAFQAEVSKLLHLIIHSLYSHKEIFLRELVSNASDALDKLK
                     YLTVSDEAYKAIAFEPGIRVSFDKEAKILTVSDNGIGMDDKDLEENLGTIARSGTRRF
                     LEALAEDVRRTTNLIGRFGVGFYSCFMVSDTVEVLTRKAGQEAAFLWTSDGKEGYEIA
                     PASRDGHGTTITLKLNEEGQEFLSRWRTEEVLKKYSNHVAYPIVLAYEQDTYDDKGKK
                     TGTERKEEQVNAASALWLRPKSELADSDYVEFYRNFADTDEDPLLWMHTKAEGTIEYS
                     TLFYVPAKAPFDLYHADYKPGVKLYVRRVFITDDEKELLPTYLRFVRGVIDSEDLPLN
                     VSREILQQNRVMTNIRNASVKKILGELKTLSESDKEKYEKFADEFNRPLKEGLYSDFA
                     NRDALLELVRFKSTSVEGWTSLADYKVRMTGDQKGIYYISGDSEERLRKSPLLEMYRR
                     QGLEVLICADEIDELVVPSIGRYGDLELKAVNRAGAEEDLGPEAKGDEAAEKAEKDLA
                     PAVERIKKALGDRVKDVRLTRRLAESPSCVVVDEADPSLQMQKLMKALGRGEGIDVKP
                     ILEINGSHPMVSRLAGTEDEDLVADTAEVLLGQALLVEGASPADPADFVKRMNKLISR
                     Q"
     gene            16236..>16764
                     /locus_tag="P5313_07735"
     CDS             16236..>16764
                     /locus_tag="P5313_07735"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRY80290.1"
                     /translation="MPESPAIPAPTLRRLPEYLGVLLDREAAGEAWISSEAIGQSLGF
                     GAVQVRKDLAALGAPGSPRRGYPVSATILRLREYPGTGGGSDAFLVGAGNLGRALLED
                     PGIVRHGFRIAGVFDLSPDRVDRILGGRKVLPLGALPDLVRRTGCRLAVLAVDPDQAG
                     AASLALVRAGITGMLD"
     assembly_gap    16765..16774
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17231..>18147
                     /locus_tag="P5313_07740"
     CDS             17231..>18147
                     /locus_tag="P5313_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY80291.1"
                     /translation="MRNRAVWIMAALLAIGLLLAGCGGKKEQVIKIATQSPLSGGMSA
                     VGTDIKNGAQLAIEQLSGPLTAMGFKVELAAFDDQGNPDTGVANAKNIVSDPAILVIV
                     GHYNSGVQIPSSEEYHAAGLANVSPANTNPRVTERGYLEVSRICGRDDVQGAVGAQFA
                     STKGIKSAYVLHDKTAYGQGIAEYFKRESEKLGIKILGFEGTEEKANFDSILTPILAS
                     KPETIYFGGMFDQAAVLFKQARQKGYMGMFLSDDGFDSSDAAKIGGDILLQGAGTFFS
                     TVAGPAAAYPGTAKFQADFKAKFGSEPQP"
BASE COUNT         2884 a         6262 c         5945 g         3046 t
ORIGIN      
        1 cgcgagctcc tggaggccga gcagtacggc ctggagctcc tccagaagct gccgggcgac
       61 cgcaacgtca agctctggcc ccccctggta cgctcgaccc tggagaaggc ggggaagacg
      121 atcgaggacc tggaccacgt gatcttcacc cagatcaacc ggagcgtcat cgaggaggtc
      181 atgggcatcc tcggactccc gatggagagg accacctgcg tgatggaccg ctacgggtac
      241 acgggctccg gctgcgtgcc catggccctc caccacgcgg tcaaggaggg ccggatccgg
      301 cggggcgacc tcgtgtgcct ggtcgcctcg ggggccgggt tcgcggtggg atccaacctg
      361 ttcgtgtact gagggatacg gagctgagaa tcgaacgcgg aggcggacaa ggccccgcgg
      421 atcgggagga accatgagcg actggaagga agaataccgc aagcgcctcg tgtcgatcga
      481 cgaggccatc cgggtgatca agagcgggga cgacgtggtc gtggcccagt gcgcctcgga
      541 gccccagggc accatgtcca ggctgcatac gatcgcggac cgggtgacgg acgtacgggt
      601 cttctcggtc ctgaccctga aaccctacga cttctatgcc aagcccgaga tgaagggtca
      661 tttcgagctg gccagctggt tccacgcccc gggatcccgg gaggccctga agcgggggac
      721 cggcacggtc accttcgtcc cgaacatgct gcaccgggcc gccacggacc ggatccacgc
      781 ccggaggccc gacgtcttca tcgggacctg cacccccccg gaccgcaacg gcttcgtatc
      841 cctctccctg gggatcacct acgagaagga catcctggag gcggcctccg cggtcatcct
      901 ggaggtgaac ccccgcctcc cccggaccct gggagatacc cacctgcggg tgacggacgc
      961 cacgtacttc gtggaacacg accaggaggt ccccgccctc cccgcccccc aacccacgga
     1021 gacggacctg tccatcggcg ggtacatagc ggacctcgtg gaggacgggt ccacgatcca
     1081 gctggggatc gggggcatcc cgaacgccgc ggccctggct ctccggggca agaaggacct
     1141 gggggtgcac acggagatga tggtggattc catgatggag ctctacgaga tgggggtgat
     1201 cacgaacaag cggaagtccc tgttcaagga caagttcgtg tgcaccttcg ccatgggctc
     1261 ccgggccctc tacgactggc tgaacgacaa cgtggccgtg gagttccgcc ggggccgctg
     1321 ggtcaacgac cccgccgtgg tggcccaaaa ctcccggatg gtctccatca acacctgcct
     1381 catggtggac ttcacgggcc aggtggccag cgagtccctg ggaacccagc agtactcggg
     1441 gaccggaggg cagtccgaca cggctcaggg cgcggtggcg ggcttcgacg gcctggggaa
     1501 gtccatcatc gcctgctacg ccaccgccaa gggcggggcc gtgagcacca tcgtcccgac
     1561 cttgccggag gggaccgccg tgaccctgca ccggtccttc gtggaccaca tcgtcaccga
     1621 gcacgggatc gcccggctcc ggggccgcac ggtccgggag cggacagggg agctcatcgc
     1681 cgtggcggac ccgcgcttcc gggaggagct cacggcccag gcgcgtaagc tggggtattt
     1741 gtaataaagc ttggaaccaa aatatagaca ggatggacag gattaagaaa cgatggacaa
     1801 gataaaaatt tgggagatgg cgacccccaa acatgaatcc tgtccatccg ctcttcatct
     1861 tgttcatcct gtctgattca tcgttcaaat cttcttctac tggcccgcgg gtcgctatac
     1921 tgtcaccatg ctccgcgatg cgcacattca cgcccccgag ctggccgagc gggaccccgg
     1981 cttcccggaa cgtttccggg cgggcgggta ccgggcctgc tccgccgccc actcggggcc
     2041 cgaactggac gcagcccggg ctctccggga ctcgggcctc gacctccggc tctccttcgg
     2101 gatccacccc cagtggacga tctggacctg ggcggaccgc ctggccgagg aggcccggga
     2161 cggccggatc gaggcgatcg gggaggcggg cttcgacttc ttcggggacc gccccgagcg
     2221 ggtgcggaac cccgagaacc tggccgccca gcgctcggcc ttcgagtacc agctgggcct
     2281 ggcggagcgg tacggcctgc ccctggtgct ccacgtccgc cgcgccctgg acctggtctt
     2341 cgagtacgcc ccccgtctca aacggatccc cgcggccctc ctccactcct ggtccgggcc
     2401 tctcgcggaa gcgagatccc tgttggccaa gggcgtgccg gcctacttct ccttcggagc
     2461 ccccgtcctg aacggaaacc gcaaggcccg agagtcctgc gccctcctgc ccgcggaccg
     2521 ggtgctcctg gagaccgacg ccccttggca gcccccccgg ggtgcggagt tctgcaccct
     2581 cgagcacctg gccctcgtgc gggacgaggc cgcccgactt cgggggatca gccccgaggc
     2641 gatggaggag tccgccgcga gagccttcga gacggtgttc gggtaaggcg ggagctgtgc
     2701 tccgataggc gaacttcgca tactacacgc cccctgctga ttaggtgaac ttcgcatact
     2761 tgcggccgta cgtcgattat atgaagttca cctgacacga accccaccgc ccatccctcg
     2821 gcaccgtgcc aacctcgaca cggcaagtcc tgtcccccac gcccgacccg tactacaatt
     2881 aaaaccagga ttccgcccgg gaggtgaacg gcatgaaaag agcgtaccga ttagccgccc
     2941 tttctctggc attcgcggca ctcctgacac ccgtcggggc gcaggaatcg gatgagggct
     3001 tcctggcgcg gatcagcgcc ggcttggacg tgatcgacat cggactggcc gcgggagtcg
     3061 gggccgtata ccggttcccg ggccccgggg gaatcgggga gatcgcggcg gacatcttct
     3121 acggccccta ctgggaaacc tatacggagg gggccaacac cttcgactat tccgagacac
     3181 tgatcatcgt agccgtccgg gccgactggc tcttcaacta cgaggcgggt gtgccgggct
     3241 ggtaccaggt cgcgggcacg ggggtcttcg cggggagcta ctcctgggag aactacaacc
     3301 gcaccacgga ctacacggag ggcaacggct acttcgcctc cggcaccgtg atcaacctgg
     3361 gcctggggta tgtattcagc cggacctggg aagcccgtct cgagatcccc gtcctggtct
     3421 ttttcggaga gtacggcgag gcagccgcga tcgccatccc gattaccgcg ggagtcctgt
     3481 tccgtccccg gtaggatccc ggttctcggt tctccgccga ccggacacag ttcggttcta
     3541 gctcgcgctc tccagcagct tccgcaggac accgtaggtg atcacgttgc tgatcccctc
     3601 cacgaacgcc ttgttctccc ggctgccgct ggctattccg ttgcggagga tcaccggaag
     3661 gagatccaga tcctgggctc cccggtaggt tgagagcacg cagtgctcgg cgcagtagcc
     3721 cgtcatcacg acgaggtcgg ggcggctttc cgccaggatc cgggcgcagt cggtcctgtt
     3781 gaaggagttg ccgtacgcct tgcggatgcg aggctcggcg ggctccggct tgagggcgtc
     3841 cagcaattcg aacccttcct tcccgggcac gacgccgtcc tcctcgtcca tgtgctgcac
     3901 ccagaggacg gggctgccgc gctttcggaa cagcggaagc accgcgttga tgtattcgca
     3961 cgcggtgtcc atggattcct tcgcggctcc ccggtaatac gctttctgga gatcgatcac
     4021 catcagggca aagttcacga cggcctcctg gcctgcggga tcgcgggcac tcccagtatc
     4081 gcacggttgc tccgtccggt ccatggatcg gacgacaggc cgcctggaac ggacgtcccg
     4141 actccggtat actctcccca tgcccttcac ggaccgcacc cgcatcctcc tcggcgacgc
     4201 cgccgtcgac cgcctggccc gggcccggct ctgcgtgttc ggcctcgggg gagtcgggtc
     4261 cgcctgcgcc ctggacctgg tgcgcgcggg gatcgggaac cttgaggtgg tggatttcga
     4321 cgtcctacag gagtccaacc tcaatcgggt cacctacgcc taccgggaaa acctggggcg
     4381 gccgaaggcc gaggtcttcc gggaggcggc cctccggatc aacccggagg tccgggtcga
     4441 gatcctcgcc tccctggtcc ggggggccga ggcggcggac cggatcctcg aaggctgcga
     4501 cttctacctg gacgccatcg acaccctgaa tcccaagacc aacctcatcg cggccttggc
     4561 cgcgcgggag gcgcccttcg cgtcctgcct gggtaccgcg ggccggatcg ccccggagcg
     4621 gctgcgcctg tcctcgatct gggagagccg gggttgtccc ctgggccaga agctgcgcca
     4681 gcgcctgcgg cgccgcgggg tgacacggga ttttccggcg gtctggtcgg acgagccccc
     4741 cgtgccgccc gccctcccgg aggacgggtc ccggcccggg gacgtcgccg acggcatccg
     4801 gatccgggcg gtccagggtt ccggcccctt cgtgccccag gcggcgggcc acatcctggc
     4861 cagcgtcgcg gtccgggcca tcctgggtct tcccttcggc gaaccccggg tctagcaggt
     4921 tgtcgaatta ctcgaagcag ttcaacacct tgctggcggc cctgccgcgc ccccagggga
     4981 agtatcagtc ccagctcgcc aggtaggctt cctgctcttt cgtcagaacg tcgatggtga
     5041 tgcccgctgc ggccagcttc aaggcggcca cgcgccggtc cacctcgccg ggcacgggat
     5101 gcacggtctt ggccagtccc gccccgttct taaggccgta ggccaggcac aggagctgaa
     5161 gggagaagga gaggtccatg atctccgcgg ggtgaccgtc cccgcagacc aggttcacca
     5221 ggcgcccctc tcccagtacg tagaccctgc ggcctgtcgg aagctcgtac tcccggacgt
     5281 tgggccgtac ctcacggaaa cccttggcgt aggaggtgag ctcccgcatc tggacctcca
     5341 cgtcgaagtg gcccgcgttg cagaggatgc acccttcctt caataggccg aagtgccggg
     5401 tgtcgacgat gttcgtgttc cccgtggcgg ttacgaagat gtcccccagg acggcggcct
     5461 tctccatagg caggacctgg aagccgtcga agtgggcctc catggccttg atcggatcca
     5521 cctcggtgac gacgacgttg gcccccaggc cccgggctcg gagcgccaga cctcggccgc
     5581 accagccgaa gcccgccacc accacggtct tcccgcagac cgtcaggttc gtggtccgca
     5641 tgatcccgtc ccagctggac tgcccggtac cgtagcggtt gtcgaaaagg ctcttggact
     5701 gggcgtcgtt cacggcgagg accggggcgg ggagtttccc ctcccgggcc agggcccgga
     5761 gccgctgaag ccccgtggtg gtctcctcgg tgatggcccg gacccgggac gccccgcccc
     5821 ggcgggccag ggtcacggtc aggtcggccc cgtcgtccag gaccaggtcc gggttgatgg
     5881 ccaggacctc ggcgatgcag gcttcctggt ccttcgggga gatcccgtgc caggcgaaaa
     5941 cctcggcccc ggtccgggcc agggcggcgg ccacgtcgtc ctgggtggac aggggattgg
     6001 atccggtgat cgccaccttg gcgcccagac ccgtcaggac ctccgccagg taggcggtct
     6061 tggcctccag gtggatggac atggccacgg atacgccccg gaacgtaccg gcgagttcct
     6121 tcgccaggcc gctcagcacg ggcatccggg gcttgaccca ttcgatcttc cgccggcccg
     6181 attcgtgaag attctcgtcc tttaacctgc tcatctgtcg tctccttgtg attcgtcgat
     6241 ccccggcggt tcagtcccgg tccgccaggt tgacggtccc ggccaggacg tcctcggtca
     6301 ggatccgcag gaatttcttc ggttcctcga acatcgggct gtgggccgag tcttcgaggg
     6361 tgtagaagcc cttcacgggc gccgccagct tcccgaggta tctccttgag aggtcccggg
     6421 ataccgtgag gtcgtggcgc ccgctcagga aatacgccgg gatgtccagg gacaggatct
     6481 cctcctccgg ccgggcggtc aggaggcggc ttcgaaggtc cgtggatcgg gccagaaaag
     6541 ccttggcctt ccagatgtcc gccttctccc gcagggtgta ggcccggctg cgccaaaccg
     6601 gcaggaagac cccggtgatc acggacttca tcgtacgggt ggtcccgatg ccgagctcgt
     6661 gcatggtctc gtcccgcaga agggagccca tgtaggacag aagaacgtcc tccccggatt
     6721 cgaggatggg gtacttccag agcttccgca gtttcttctc gttccccgcc tccatgtacc
     6781 ggtccagcat atacgcgtag gcccgcttct ccgattccgt ctggtccgcc acctgggcga
     6841 cgccgatgta ggcgaggaat ttctccggcg cccggtcggc ggccttgatc gccaggaaac
     6901 tgccccagga atgccccagg aggtagatct tatccttccc gaaccgagag cgcaggtagt
     6961 cggtcgcctc caggacgtcc gagaccaggc ggtccacggt cagggtcccg ggatccatcc
     7021 cgggggagta cgagagcccg gctcccctct gctcccacca gcagaccgtg aagcggctct
     7081 ccaggccggt cgggtacagc tcgtccagga agtactcggg catgcccggg cccccgtgaa
     7141 cgaagaggag aaccgggttg gcgggatccg cggagcggat gaacatgccc tgccggacgc
     7201 ctccgatgtc gacgaagatc ttctcggaga tcgagcccgg cactggacgt ccgtcgacac
     7261 ccagtacggg catcggcacc cccgggctcc gggaccgcag gagggcccag cctccgagcc
     7321 ccagggccag ggccaccgct accccgaaca tcagcacgcg agccgccttc cgcaccgcgg
     7381 gttcagacgc cctcccggtg ttctccgtat ctcccatcgt ccctccatta tttcctattc
     7441 cttcctcatc acttgttcat ctctcttcaa tcttgttcat cctgtcggat ctatccggaa
     7501 aacgccttca agacttcttc cccagcccga gaaggtaggt ctcgaagctt tcggctctgc
     7561 aggccttggg cttgaacccc cgggccgtct cgaagcggcc tcgcagggcc tgcagcaggt
     7621 cccgctcccc tccgccctgg aagagtttgg ccacgaggtg ccccccggga gccaggaggg
     7681 cgtccgcgta gccgatcacc gcctccacga gggcctcgga tcggccggtg tccacggtcc
     7741 ggtccccggt ggtggagggc gcggcgtcgg agaggatgag gccgtagggg ccccgctccg
     7801 ccagggcggc ccggacggcg ggatcgtaca ggtccccctg gaggaaaaag aagttggcga
     7861 aggggggctt catccccagg ggcgagaggt ccacggcggc gaggaaaccc gaacccttca
     7921 ggaagcgcag gacccagagg gaccaggagc ccggggcggc gccgatgtcc aggacccggg
     7981 tccccggctt gaggagcttg aacttctcct gcatctccgc cagcttgtac acggaccggg
     8041 ccggatagcc ctcgctcttg gccttgaggg tccagtggtc cggcttctcg tagctgttcc
     8101 ccgacatgcg cgccccttcg gattgattcc ccggtccggc tccgccccac ttctgccttc
     8161 aacccggtcc tggtagaatg acctatgcga ccagagtata cggaaatcct tgcaaagata
     8221 gaagccgagc tgacccggga actcccggaa acacccgccg acgggtggct ggagagccgc
     8281 ttcggccccc tggcgtccct gccgaacgag gcggaccggc aagccgtgac cgccccgggc
     8341 cgcgagctgg tgctccgggg agggaagcgc tggaggcccc tcctgcaggt cctgtgcgcc
     8401 cgggccttcg gctccgggga cctggccctg gccctggccc ccctgccgga gatcgtgcac
     8461 aacgggaccc tcatcgtgga cgacatcgag gacggggcgg aggtccgccg cggaggtccc
     8521 gccgtgcacc tggcacacgg cctggacgct gccgtcaacg gcgggaacct gatgtacttc
     8581 ctgcccctgg ccctcctgga agaccgcgtc ccggaccccg tcctgcgagc ccggctctac
     8641 ggggcctatg ccgcccagct ccgccgggtc cacctgggcc aggccctgga catcgcctgg
     8701 caccgggacc ccgggcgcct gccctcggag cccgagtatc tcctgatgtg ctccctgaag
     8761 accggggccc tctcgggact cgccgcccgg atcggcgccc tctgcggggg cgccccggac
     8821 gcagccgcgg acgccctggt ccgggccttc gaggaggcgg gggtggcctt ccagatcctg
     8881 gacgacgtcc ggaacctgag gaccggaaac cccggcaagg agcgggggga cgacatcgtg
     8941 gagggaaaga agagcctgcc ggtcctccgg gcctgcgccc gggacccctc cctggcgggc
     9001 cgcctgccgg ggttcttcgg ccgcgcgaag gccgggggcg tccgggaccc cgcggtggaa
     9061 gaggccgtcg cggcgatatc cgctacgggg gctctggagg aggccgaggc cgaggcggcc
     9121 gggctccttg cccgggcgga ggcggccgcc gggacggcct gccctccggg cccggggcgg
     9181 gagtccctgc tggggatctt ccccctgctg gcggggaagt gagtccccgg cgcggatccc
     9241 gcaggtagca gggggattac tttccgccgc gcgcccgcgc cacgatggcc cggaagtcct
     9301 cgtccccgac caggccccgc cgctcgttcc ccaggcgctt cacctccgcc agcaccgcgg
     9361 cggccgtctc ctcgtccaat tccccgaggc ccagctccgc gagcttgtag gtcaccgagg
     9421 gcttgccgct cttcttgccc aggacgatct tccccttccg cccggtgagg gccggatggg
     9481 tggcgaacat ggccagaggt tcctccacga ccaggttcac gccgatgccg gactcccggg
     9541 tgtagttctc ctcgccgacc accggcttgt tttgcgcgag ccgcacgccg gaaatctcct
     9601 ggaccagggc ggacaggccc aggagggccg ccgggtcgca gcccgtgccc tccccgtaca
     9661 ggaggtccag ggccaggatc agctcctcga gggccgcgtt gcccgtgcgc tcccccaggc
     9721 cgttcacgca ggaatggacc acctcggccc ccgccccgac ggcggccagc tccgtggcca
     9781 cggccaggcc gaagtcgttg tgggtgtgga tctccacggg caaccccgtg atgtcccggt
     9841 acttgcgggt gaggtacgcg atggcctccg gggacgcgca ccccatggta tccacaaggc
     9901 ccacggagtc cggcggggat tcccgcatga tcgccgggag gagggcgtcc aggtcctcct
     9961 cccgggcgcg ggtcgcgtcg taggggaaat acacggtata caggcccttg gacttggcgt
    10021 aattgatgac cggcacactc ttccggagca cgtcctccca ggtccatttg aactggtgga
    10081 cgagcttcgg gtacccgatg gggatctcga tcaccacgcc gtgggctccg cattccacgg
    10141 ccttgtcgat gtcctcggtc atcgtccggg cgaaggagaa gatcttcgcc ttgagcccca
    10201 gggcggcgat ctcctcgacg gcccggaagt cctcctcgga caccgcgggc atgcccgctt
    10261 cgatcctgtc caccccgacg gcgtccaggg ccttggcgat ggcgaccttg tgctccttgc
    10321 ggaacaccac gccgggggtc tgttccccgt cccggagggt cgcgtcgtgg atccgtacct
    10381 ttcctggacg ctcgcggccc gcgaggacct cgggcttgaa gttgtagggg ctcacccacc
    10441 attgtccgtc gctcttgtac gaagtcatcc gtcctctcct ttgtcgtccc tcggttcagg
    10501 ccgaggcgcc cgtggaatcc cgtcccgccg aggcggacgc cgccttctgg atacgctccg
    10561 cgaccgcggc gatgtgctcc cgcatcaggt cccgggccgc cgccgcgtcc cggttcccga
    10621 cggcttcgac gatcctgcgc tgggactcga tcgtccgggc ggtcacgccg ctcgccggaa
    10681 ccgtcgtctc ccggctttcc cggagtatgt cccgcaggga ttccaggatc ctccccagca
    10741 cggtgttgcg ggaagcccgg gccaccaggt cgtggaggcg gagctcgcat cggatgccgc
    10801 gctcccgatc ggcaccggaa gcctcgagct ccgagagggc cgtccggagc tcctccacct
    10861 cctccgccgt cgcgcgctcg gcggccagac ccgcgcattc cgtctcgatc accaggcgcg
    10921 cctcgaagag ctcctggagc ctgtcccggt tcaggaggag ggcgaaggag aggtgccggg
    10981 tcacggaatc ggatacccgg gaggagtcga tgaccatgcc cgatccctgg tcgaccctca
    11041 ggatgcccag catgtccagg acgcggatcg cctcccgcag ggaattccgg gaaatgccga
    11101 gcatctcgga gagccggcgc tccgggggaa gccggtcgcg ctcccggagg ttctccgact
    11161 tgatgagctc gtagatgcgg ccgatggcct gctccacgac ggaggtcttc ttgatcggtt
    11221 cgaccatggc gttccgggcc ccccaagcca ccgtacatcc gcccccgcga gttgtcaagt
    11281 catcctacca ctttggcatt tgcatccccc gccgcctccc gctagacttg aaccgtggct
    11341 acgaacaccc tcgtggaggc ggacatctgg accgtcgccc agacggacca gggcaacgtg
    11401 gtcctggtgc gccccaagcg gtcggagctg gcggttccca tcttcatcgg ccaactggag
    11461 acccagtcca tcctcatcgg cctggggcgg gtcgaggttc ctcgcccgct cacccacgac
    11521 ctcttcctgt cgaccctcaa ggccctgggg gcggacctga cccggatcga gatcaacgac
    11581 ctccgggagg gcaccttcta cgcccggctc atcctggccc ggggggagga gaccctggtc
    11641 gtggacgccc ggccctcgga cgccatcgcc gcggcggtgc gggcgggctg cccggtcttc
    11701 atcgccgagg ccatcgtgga ggaggcgggg atctcggtga acctcgtctc cgaatcctcc
    11761 aagctcgaag ccgcgggatc cgaggacgag aaggagcggc tgcgcaagga actcgaggag
    11821 gccatcggcg aggaggacta cgagcgggcc gcccgtatcc gggacaagct caaggaactg
    11881 gagggctgac cccggccgcc gcgccccttc ccggaggttg acgtttccca tccccgccgt
    11941 catacttccg ccccgaggcc gatcggccgt acaaggagca cccggtgctg aaaagccgcc
    12001 gcagggatgg aaccctcctt cgggacctgc ccgcgttcac ccgcatcatg ccctacctga
    12061 tgcctgacaa ggcgggctcg gtcatcttct accaggaaga ctacgacgtc accgagtgct
    12121 tggcgtacat caaggattgg aaccgccgga acgcccggac cgcgggcgcc ctcctcaccc
    12181 ttttcgaggt cgtcctggtg gcggccgccc gggccgtggc cctgcggccc cggatcaacc
    12241 gcttcatcgc caaccggcgc tactaccagc ggaaccagat cctgctgtcc ttcgtggcca
    12301 agaaggagct ctccgacgac gggaaggagg tgaacgtcaa ggtgcccttc gacccggagg
    12361 ataccctggg ctccgctgcc cggaaggtcc gggactacat caagaagggg atctccgagg
    12421 acggccacga gaacgagaag gtcgtggact ccgtgatgcg cctgccctcc ttcctcctca
    12481 agttcatcaa cttcgcctac aaccggctgg atgaccacaa cctcctcccc tactcggtca
    12541 ccgagagcga ccccatgtgg tccacgatct tcctggccaa cgtggggtcc ttcggactgg
    12601 acgccccctt ccaccacctc ttcgagcggg ggacctgccc catcttcatg gccgtcggga
    12661 aggtccggga cgagaaccgc gtctccgagg ccccggacgg gaccccgagg gtggagaccc
    12721 ggaagatcct ccgggtgaac tactccttcg acgaccgcat cgcggacggg atctacatgg
    12781 gccggtccct ggagctggtc cggaatttcg tggaggatcc cgccgccctc gaccgcccgc
    12841 cggagatacc cccggagatc cgggcggagc tggccctgcg ccccgcaggg gactgaccgg
    12901 acggcccgga ccacgggcgc cggccttgcg gcgccgcggg cggggctcta gacttgaatc
    12961 gtgaaagcca catcctcgtt ccgcccgttc ccggccctgc tcctggccct ggccgcctcc
    13021 gcggcctggg cccagggagc cgcatcgtcc ccgggaaccc cccttccggc cgcggcggat
    13081 cccccgcgag ccgtggcgct gatcgccaag tactgctcgg cctgccacgg ctgggcctcc
    13141 ggctacaagg agatcgccgc ggaaggcgtg atcgtgccgg gaaagccctc cgagagcagg
    13201 ctgttcgagt acgtcgagtc ggggtacatg cccccctctc ccccggcccc gtcccccgcg
    13261 gagatcgaac ttctgcgggc gtggatcgct tcgggagccg cggctccgga ggcggtctcg
    13321 ggggccacgg agagcgcgga cgccgggagc gccgcgtccg cgggggctcc gggtaagggc
    13381 ggtggattcc tgggcttcaa gagcaaggtc gactaccacc gggcggcggg ctggacctcc
    13441 tcgggcctgt tgttggccgc cggggtggtc ggggcggtgc gggcctacga cctgatggat
    13501 gcggcgcacg actaccggga cgctatgggc atcaccgagg atacgatgga tcctgtctgt
    13561 tacgacatga tccgggatct ctggtccgga gaccaggccc tgcggtggac gcacgtgagc
    13621 ctgctggcgg cgggggagac cctctacctg ggcaacgcga tcacggggat gtccatggcc
    13681 cgcaaggcca agccgggcca ggtcagcaag tccgacatcc accgctgggc cttcttcacc
    13741 cacgcggccc tgatggcctc ggagatcgtg atgggcctgt tcaccacgga cgccctcaag
    13801 aggggggacc atgagacggt cgcgaacctc ggcagagccc acgctgcggt gggactcgcc
    13861 atccccctgg tcatgatcgg ggcgggcatc gtgatcgagc tgccggcccg accgaagacc
    13921 gcccccccgg cggagtagaa atccgtgggt ttcaggaatc accacggagg acacggagct
    13981 cacggaggat acgaagaaga ttcggccttg ggacactaga tccaacaaga tggacaagaa
    14041 aaagaataga tgaacaagat aggattagga atagaatcga ttccttatcc ccatcttgtt
    14101 catcctgcat catcctgtag atcctgttgg atctatggtt ccaaatctct tctcctgagc
    14161 agggtcactg ccgggagata agcttgttca tccttttcac gaagtccgcg ggatccgcgg
    14221 gggaggcgcc ttccacaagg agggcctgtc ccaggaggac ctccgcggtg tcggcgacca
    14281 ggtcctcgtc ctcggtcccc gccaggcggg agaccatggg gtgagacccg ttgatctcca
    14341 ggatgggctt cacgtcgatc ccctcccccc ggcccagggc cttcatgagc ttttgcatct
    14401 gcaaggacgg gtccgcctcg tccacgacca cgcaggaggg gctctccgcc aggcggcgcg
    14461 tcaggcgcac gtccttcacg cggtccccca gggccttctt gatccgctcc accgcgggcg
    14521 ccaggtcctt ctcggccttc tcggcagcct cgtccccctt ggcctcgggc cccaggtcct
    14581 cctcggcccc ggcccggttc acggccttga gctcgaggtc cccgtagcgg ccgatggagg
    14641 gcaccacgag ctcgtcgatc tcgtccgcgc agatcaggac ctccaggccc tggcggcggt
    14701 acatctcaag aagggggctc ttccggaggc gctcctcgga gtcgccgctg atgtagtaga
    14761 tccccttctg gtccccggtc atccggacct tgtagtccgc caggctcgtc cagccctcca
    14821 cgctcgtgct cttgaagcgc accaactcca gcagggcatc ccggttggcg aagtccgagt
    14881 acagcccttc cttgaggggg cggttgaact cgtccgcgaa cttctcgtac ttctccttgt
    14941 ccgattccga gagggtcttg agctccccga ggatcttctt gaccgaggcg ttgcggatgt
    15001 tcgtcatgac ccggttctgc tggaggatct cccggctgac gttgaggggc aggtcctcgg
    15061 agtcgatgac gccccggacg aagcgcaggt aggtcggcag gagctccttc tcgtcgtccg
    15121 tgatgaagac ccggcggacg tagagcttga ccccgggctt gtagtccgcg tggtagaggt
    15181 cgaagggggc cttggccggc acgtagaaga gggtcgagta ctcgatggtc ccctccgcct
    15241 tggtgtgcat ccaaagaagg ggatcctcgt cggtgtccgc gaagttcctg tagaactcga
    15301 cgtagtcgga gtccgccagc tcggacttgg ggcggagcca gagggcgctg gccgcgttga
    15361 cctgctcctc cttgcgctcg gtcccggtct tcttcccctt gtcgtcgtag gtgtcctgtt
    15421 cgtaggccag gacgatggga tacgccacgt ggttggagta cttcttgagg acctcctcgg
    15481 tccgccatcg ggacaggaac tcctgcccct cctcgttgag cttgagggtg atggtggtgc
    15541 cgtggccgtc ccgggaggcc ggggcgatct cgtagccctc cttcccgtcg ctggtccaca
    15601 ggaaggccgc ctcctgcccc gctttccggg tcaggacctc cacggtgtcg gaaaccatga
    15661 agcaggagta gaagcccacc ccgaagcggc cgatcaggtt ggtcgtcctc cggacgtcct
    15721 cggccagggc ctccaggaag cggcgggtac cggaccgggc tatggtcccc aggttctctt
    15781 ccaggtcctt gtcgtccatg ccgatcccgt tgtccgagac ggtcaggatc ttggcctcct
    15841 tgtcgaagga gacgcggatt cccggctcga aggcgatggc cttgtaggcc tcgtcggaca
    15901 ccgtgaggta tttgagcttg tcgagggcgt cggaggcgtt ggatacgagc tctcttagga
    15961 agatctcctt gtgggagtag agggaatgga tgatgaggtg gagaagcttg ctcacctccg
    16021 cctggaacgc tttctgggcc atggttcact cctagggatg cgtgggatct cgggtatgaa
    16081 taggaaaata ctgatcggga tcccggggcg gcaagggagg cgtcgcgcga ggaatcggcg
    16141 ggcgggagct cacgagcccg ggacctcccg gcgccgcccc atcgtcgccg gtttccggct
    16201 ggcgctcccg gaccgggagg gcgtatactg gacggatgcc ggagtccccc gcgatccccg
    16261 cgcccaccct gcgccgcctg ccggaatacc tcggggtgct cctggaccgc gaggccgcgg
    16321 gggaggcctg gatctcctcc gaggcgatcg gccagagcct gggcttcggg gccgtccagg
    16381 tccgcaagga tctggccgcc ctcggagccc cgggaagccc ccggcgcggg tatcccgttt
    16441 ccgcgacgat cctgcggctc cgggagtacc cgggtacggg cggaggctcg gacgccttcc
    16501 tcgtgggcgc cgggaacctg ggacgggcgc tcctggagga ccccgggata gtccggcacg
    16561 gcttccgaat cgccggggtc ttcgatcttt cgccggaccg ggtggaccgg atcctgggcg
    16621 gccggaaggt ccttcccctg ggggcgctcc cggaccttgt ccggcgcacg gggtgtcgtc
    16681 tggccgtgct ggccgtggac cccgaccagg ccggggccgc atccctggcc ctggtccgcg
    16741 ccgggataac cgggatgctc gaccnnnnnn nnnncgggat ccgcctgccc cttcccccgt
    16801 accctgatcg tacggcggat cggcatcgga gccggattgg ccgccctggc cggggaactg
    16861 gaagcccggg aacgagaaaa aaaccttgac ggctttccaa ggcagacata gactcctgat
    16921 atatttgttg gtttttcggg cggggatgcc cgaaatgaga caaatggatt gacggcaaag
    16981 gaggccgttc aatagactcg gaatcggttt ctctttatag aaaccaaatc gagcgttgga
    17041 cgatagtccg ccgtccttct gcagcgtgcg taccacggct cggcggtcat cactcaggcg
    17101 acccacccca aggaggcggc cggagatcga gagaccatcc atctcatcag ctcacccaga
    17161 cgacaggaag agcgcgcgtc gcgcgcaaac ggagtccaga actcgttgta tcgttccggg
    17221 aggattgtgt atgagaaatc gcgcagtgtg gatcatggcg gcgcttctgg cgatcggcct
    17281 tctcctcgca ggctgcgggg gcaagaagga gcaggtgatc aagatcgcca cccagagccc
    17341 gctctccggc ggcatgtccg cggtcggaac ggacatcaag aacggcgccc agctggccat
    17401 cgagcagctt tccgggcccc tcacggccat gggcttcaag gtcgaactgg ccgccttcga
    17461 tgaccagggc aacccggaca ccggcgtggc caacgccaag aacatcgtct ccgatccggc
    17521 catcctggtg atcgtcggcc actacaactc cggcgtccag atcccctcct ccgaggaata
    17581 ccacgccgcc ggcctggcca acgtgtcccc ggccaacacc aaccccaggg tcaccgagcg
    17641 cggttacctc gaggtcagcc ggatctgcgg acgcgacgac gtccagggag cggtgggcgc
    17701 ccagttcgcc agcaccaagg gcatcaagag cgcctacgtc ctccatgaca agaccgccta
    17761 cggccagggc atcgcggagt acttcaagcg ggaatccgag aagctcggca tcaagatcct
    17821 gggtttcgag ggcaccgagg aaaaggccaa cttcgactcc atcctgaccc ccatcctggc
    17881 ctccaagccc gagaccatct atttcggcgg catgttcgac caggcggcgg tcctcttcaa
    17941 gcaggcccgc cagaagggct acatgggcat gttcctctcg gatgacggct tcgactcctc
    18001 cgacgcggcc aagatcggcg gcgacatcct cctgcagggc gccggcacct tcttctccac
    18061 cgtcgccgga cccgcggccg cctaccccgg aaccgccaag ttccaggcgg acttcaaggc
    18121 caagttcggc tccgagcccc agccctt
//
LOCUS       DAOTEI010000061        18074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2086_length_18074_cov_5.666963, whole genome shotgun sequence.
ACCESSION   DAOTEI010000061 DAOTEI010000000
VERSION     DAOTEI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18074
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2086_length_18074_cov_5.666963"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..1107
                     /locus_tag="P5313_07745"
     CDS             382..1107
                     /locus_tag="P5313_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY80292.1"
                     /translation="MPDLPAIGPFSPADRRLLKRYEGVADAVATLFGRGCEIVLHSLE
                     DLSHSVVKIVNGHVTGRGLGSPITDLGLKVLRCSFEGTEDVIGSYFAFTDSGRPIKSV
                     TLLIRNDRGIPIGMLCINFDLSTPLASFIKEFSPFQDLPASSEHFPAGVGDLVQHALA
                     EEAEHLARANGIPIQEKNRRIVEGLENRGLFEIKGAVESVSKGLGITKYTIYKYLREF
                     RKSRLPADGSGAMNPGTSWSSPD"
     gene            1248..2501
                     /locus_tag="P5313_07750"
     CDS             1248..2501
                     /locus_tag="P5313_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY80293.1"
                     /translation="MARTGNYIDEHIGEIRKEFKLLDYWVYLNSGDQMIPGNYWLKAA
                     REFYDFVEFGRMEDIPVADIATHPFLTTAWNDCIELGARFINADPEEVTNTYRPAITA
                     NLLLYNCFDWKAGDNVVITDLNYPSIVYILMDIRKRYGVELRVVKNVGGEILLEDMEK
                     AIDGRTRMVCVDRTAAFGGFTYDMKALCKIAHAKGALVFDDAIQALGAIKIDVKDDDV
                     DILVSGAYKWQCGPEGAGLLYVKKDLIRKLDPRYRDYLWADIPTGIPFALKDHDNMRH
                     WDFPPAETANKFSQDVTIGPALFGWKETLRFYEKIGMENAEERIKRLGTYLIERLEEI
                     GCVVKTPKDVRKRHGMIVYTTGSYETDLKSFQRFAAPGRCMKPVKVSMRALGGVGNIR
                     ACTHFYNTEEDVDYLIRVQSAVMKA"
     gene            2698..3078
                     /locus_tag="P5313_07755"
     CDS             2698..3078
                     /locus_tag="P5313_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRY80294.1"
                     /translation="MMRTKIETPDAPAAIGPYSQAIRYGDLLFVSGQLPIDPAKGAME
                     APDIAVQTRRVLSNLTAVVRAAGMKPENILRCTCFLADMNEFPKFNEVYAEFFGKDAP
                     SRETVQVARLPKDSRVEISAVCGA"
     gene            3081..3830
                     /locus_tag="P5313_07760"
     CDS             3081..3830
                     /locus_tag="P5313_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRY80295.1"
                     /translation="MVRGGFVTYGKVAGILMLDSRIPRIPGDPGHAQTFPFPVAYRAV
                     EGLPFSDLVEGRLDNADKATEAAASLEADGVSFVAADCGLFSVLQKKIAARLSIPFLG
                     SALALIPLLGTFLPPNRCVGVLTGHTDLLRDHHLRAAGVSPDGIVVAGMENSSEFRKV
                     VIDRGTDLDPEAMREGVREASLELAGRASRKGLGLGAVVLECTNLVSFRDTVQEAAGA
                     PVYDLVSLVELYASGYARREFAETHRSFARG"
     gene            3896..5239
                     /locus_tag="P5313_07765"
     CDS             3896..5239
                     /locus_tag="P5313_07765"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRY80296.1"
                     /translation="MAEQTNRETFASKFGMIMTLVGVSVGLGNVWRFPYMAGKFGGAA
                     FVFFYVLLLFVVGIPGLIAEYNLGRYTNRGTLGAFQKAGFPGGKVVGTWFWIVTTFAV
                     GYYCNVIGWVLFYAVREVGVAVGATVNAAAILPPDTGFNATSFFLQFFFTGIVILSCA
                     LVLAKGLKEGVEKASKYIMPAFFALFLILIVRAVTLPGAGAGIKWYIGDFRWKELTGG
                     SMAAAMGQVIFSLSLGGTFMVVYGSYLKKDTNLVQTGIMNGIGDTIAALMAGFAIFPA
                     VFAFNVEPSSGPGLIFFTLPKVFDSMPAGWLFAIFFYMALFGAAYLSAIAAFEVMIAG
                     IVDNTKLNRKQATWLMAGLGFLFAIPPMINFQIFGPWDLFWGSGMQTLGCVLAVIASV
                     WVIKRSEGLKALAEGTGKQFPIWLYWWMRIAVPLAVGFVGVNWLLSEVFKINIFG"
     gene            complement(5358..6266)
                     /locus_tag="P5313_07770"
     CDS             complement(5358..6266)
                     /locus_tag="P5313_07770"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY80297.1"
                     /translation="MADEKRTAAAIAGNCFAMLFYGLSFLWTKVAFKYCGPFTVIFVR
                     LVLSSAFLAIVDALRIRGRTASPGHSRIERKDFVSLVILSLFMPFLYFVGENLGLMSV
                     SPAVAATIIALIPVVTPLMSRIFLAERVGILAVAGMILSLAGVLLMIKNGLADASATV
                     GGACLVFLAVLAASLGAVAVRKLPLRLSGITIVKYQNFFGALMFLPLFLFYEAGEAVL
                     ILPAPELYWSLGALALLPSTVSYLFYNNAIRVLGPARASVFSNAVPVIAAAFSAIVLG
                     EKFGAVKILGMGIALVGVAISQIRNS"
     gene            complement(7056..7880)
                     /locus_tag="P5313_07775"
     CDS             complement(7056..7880)
                     /locus_tag="P5313_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80298.1"
                     /translation="MNPTALSARGLACGYRRVPVLRDVDLDIPEGELTVLVGPNGSGK
                     TTLLRSLGGLLAPLSGEILVRGRPIARLGIRDRARLAAYVPQMEAPVPGYTVRQTVWA
                     GRFARLGWFAPGTPEDERAVAGALAAVGLGGDAERPASELSGGEFRRLLIARALAQEA
                     PVLLLDEPEAHLDAGHQAEILGMLRRLARSGTAVAATIHDLNLASLWADRIGVLTGGR
                     LRPPAAPREALSEDAVEGAYRAGLIRIDHPRTDRPQFLHSVTNLVMTQARKRNRAE"
     gene            complement(7877..8923)
                     /locus_tag="P5313_07780"
     CDS             complement(7877..8923)
                     /locus_tag="P5313_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY80299.1"
                     /translation="MKARSLRTASVPSRAARRPVVRLAYALAALGLLVLVLLASLGMG
                     AVSYGPREVAAVLLGKQGADPVAAVILRDLRLARTLAAGLAGAALAVSGVLLQGLFRN
                     PLADPYILGTSAGAALGAAAALVFVRTPGSASLGAFAGALGAAALSGAAARATSRSGS
                     TTAVLLAGTAVSALCSAGVTLLVSLRQQDLYAVYFWLLGSFNGRGWKELAVLAPLTAL
                     VSGTALLLARPLDVLAAGEESARSLGLDVRLLRAAAVALAALGTAAAVAAGGVVGFVG
                     LVAPHAARLVFGPSHARLIPASAVAGAVLLMSADALARTVLAPLELPVGVLTAFAGAP
                     FFLVILARRDGGRR"
     gene            complement(8920..9828)
                     /locus_tag="P5313_07785"
     CDS             complement(8920..9828)
                     /locus_tag="P5313_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRY80300.1"
                     /translation="MSRSTHGVAILLAGLTCLASVAWGQTLSARDSLERTVTLSGPAR
                     RVVSLNPSATESLFGIGAGDRVAGVTSYCTWPAEAARVPRVGGYTSESISLERLLTLK
                     PDLVVTGGPVHVSLRKSLDSLGISYYVFEPSSVEQTLECLADLGVLTGTAEGAARSIA
                     GLRRDLAEVTARVASIPAASRVRVFWEVYNEPLMTCGAPSFLHELITQAGGINVFAEM
                     TQPWAIVSPEEAVRRAPQVILAADDHAPGFTAGDLARRPGWGTVPAVRTGRVYMLPAD
                     PVNRTGPRLGEGVRSIARALYPELFP"
     gene            complement(9825..10931)
                     /gene="cobT"
                     /locus_tag="P5313_07790"
     CDS             complement(9825..10931)
                     /gene="cobT"
                     /locus_tag="P5313_07790"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRY80301.1"
                     /translation="MSRTSLSSRSSTSLLETTLSRIGLPDAAAAEAARSRQARLTKPS
                     GSLGRLEDLAVRIAGIAGNSVPELGPGVAFVMAADHGVAAAGVSAYPREVTAAMVRNF
                     LAGGAAVNALAAFAGFRVSVTDCGVASDLGPATGLRIRRVRPGTDDITRGPAMSRDEA
                     RRSVEIGIEAFEEEFEFGPFGLAAAGEMGIGNTTPASAIVAALTGRPPREVTGRGTGV
                     DDRGLERKVAAVERALAVNRPDPSDPLDVLSKVGGFEIGAMAGVFLAAAARRIPVLVD
                     GFISAAAAMIACGLCPLAAEYMIAGHRSVEPGQAAALGLLGLTPVLDLGMRLGEGTGA
                     VLAAAVCRAACEVHTRMATFEEAGVPGAPEGKKT"
     regulatory      complement(10966..11169)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11291..13099
                     /locus_tag="P5313_07795"
     CDS             11291..13099
                     /locus_tag="P5313_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY80302.1"
                     /translation="MKNQARPVRAAGLLLAVLLAAAPVTASDGFERERFLILNSYHHG
                     FEWSDGILRGILEVLSREFARPALHIEYMDTKRHFDGIDGPYLDGLAAVYAAKYTRTK
                     FDAVLCTDDAAFLFLLRWGRTLFPGTPVVFTGVNNFSPDLVAGRPEITGIVERTEYGP
                     SVELGLRLRPEARRLGVITDASLNGLANRAKFEALAESFRGRLEFVFFRTDGLSELLE
                     EVRAFDPGGLIYFSDFYLDGRGRSYEYDVSVPEVLAAAPCPVLYHADMYLPFGVLGGR
                     MNRSREYGELAARMAARIVRGEPPSAIPVMQDSIARAVVSYPALRKFGIPESRVPPGT
                     LVADKPESFLRRNARLLTVSGIITLSLSGVIVSLLYAIRERRRAERDLHASRETILQS
                     LREKEVLLQEVHHRVKNNLQIVTSLLRLQSAHSGKPEVEEALTESTERLMAMALVHEQ
                     LYLSKNFADIEYSEYAAGLVSSLRATFGLSEEAFRCSIDSSGLRLGLDTAVPLGLILN
                     ELLTNSVKYALPARSPLSVRSLLEEADGMLRFRYQDDGPGLPVGLDPEKDGGLGLTLI
                     TALSQQLRGAPAFPRDRAGMVFELDFTPRFPPRGKI"
     gene            13220..14776
                     /locus_tag="P5313_07800"
     CDS             13220..14776
                     /locus_tag="P5313_07800"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRY80303.1"
                     /translation="MFARFGAIVSIAASSFALAVMMVSAALRSRTRGRTLSFAGLCAS
                     ALVYSLGYLLELLAPDLEAALRVLRFEYIGIVTIAPFLTLVAYEFLDEGAPRVPAPLL
                     FVIPAGILALAWTTQLHRLYYLDPVLTRVAGLSVLEFGRGPAYWVHVLYQNLLIAYCV
                     TIFLRNTFRGSRARRVQARFMLAGCVLPWGGFVAYLAGGVPLNLDPTPVTLAFTGIFF
                     AVGFFRYKMFDLRPIARDTVFEQMRDAVLVTDDQGAVVDHNTAARRLFPALASFRGIR
                     TARDLARDAPDLAEAMSRSASDSVVTLGLPEGDRSFNLHHSALSDRGGRRVGTANVLT
                     DITERLLLEDRLTVQATTDELTGVANRRHFFDRARVELERAQRYGRPFGVAILDMDDF
                     KSINDNFGHPAGDEALRIASRLCAETLRSTDIMGRYGGDEFAFAFPECDEDQAREAAE
                     RLSRILASAAFPYGDTVIRLSASVGYAGGSEPPLPDLDSLLKLADERMYEKKGRPPSF
                     KSVARDPDRD"
     gene            14891..15373
                     /locus_tag="P5313_07805"
     CDS             14891..15373
                     /locus_tag="P5313_07805"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRY80304.1"
                     /translation="MRTLIIGGVKSGKSRRALELARAFPAPRTFVATAQAFDPEMAEK
                     IARHRSERGDDFETVEEPLRIDRAVREPCVLDCVPLWLNNLFYENRESEAEGILDSLL
                     ERLPASIVLVTNEIGMGTIPMDPLSRRYGEALGRINARLAAAVDRVELMVAGIPLRVK
                     "
     gene            15375..16136
                     /gene="cbiR"
                     /locus_tag="P5313_07810"
     CDS             15375..16136
                     /gene="cbiR"
                     /locus_tag="P5313_07810"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HRY80305.1"
                     /translation="MILSVPSYVIPGTYAENLRFLADKPEVEGVELLFYLWDAEVAGL
                     LDRELAEIRSFMDRFRFTAHLPDRILPEHRVLLDRIADTARSFVVHPPSDLDGLAPFA
                     DLLSVWKSGFGDRFFLENLRSEPFGRALRVLPDIPLCVDAGHILAEGGDPAELLARLR
                     STGRRIEELHLHGLSGDADHQPFGEGASWLRDLAPFLAGFDGVAEIELFDWEEARTAL
                     AAVRRAGVEAAASEAAAGPRSGRTARAAAPGKETE"
     gene            16133..16933
                     /locus_tag="P5313_07815"
     CDS             16133..16933
                     /locus_tag="P5313_07815"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRY80306.1"
                     /translation="MSSPLDPFLSVLALTCRIRVPAPAEPDYGRADFFLPLTGVLPGL
                     AALGGWGLGSLVFPGPWLPALSALAAQYLAFNLFHLDGLADSADAGLALASPERRLAI
                     LKDPRVGVYGLFAAFLVLAARTAGLAALGGRPGFPAVLLAAPVAGRFAAALVPLTARP
                     ARPDGLGARMRDFRPQAVASGLLAGLAPAFLAVLAGLPGEGSVLAGILGTGAGALAAG
                     LAAGLGTAIFYRRRFGGFTGDALGAAVELGELAFLLGACAVAGTAGGA"
     gene            16935..17972
                     /locus_tag="P5313_07820"
     CDS             16935..17972
                     /locus_tag="P5313_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY80307.1"
                     /translation="MELLDFRSNLNPEGPPPWLAEAEEAARTQSAPAVSGFRKAKEAC
                     FRRFSFSPDCFVFGEGVNGLLSVLPRALGVDAVLILPPTYSGFQRAAAAAGLPLHLHA
                     AREEDGYTLRPGSLRADMARISGRALVFLGRPNNPAGVPLPIETVIEAARVLPGHFLC
                     VDESYVELSEGVEPVLARGRRLPDNLLVLRSLTKALAIPDLHLGFVGASRTVTRRIED
                     ALELRPRSPRAEEIAFRAVSDPRFFETSAARIAGYRARFAERLSRIPGVKPETGFANF
                     LLVRIQGADARKAAAAARERGVDFRDYAEDDYLDGRRWLRTAVRKDEDNEKAATALEA
                     ALREVREAGKA"
     gene            complement(17969..>18074)
                     /locus_tag="P5313_07825"
     CDS             complement(17969..>18074)
                     /locus_tag="P5313_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY80308.1"
                     /translation="RTAIRTALSGNLCRCTGYQQIVDAVELAAARMRG"
BASE COUNT         3029 a         6109 c         5939 g         2997 t
ORIGIN      
        1 atccaggtcc cccaaaaatt ccccgagagt cggggccgga gccatggaaa acacgacgga
       61 ttcgcggggc tccgccgcat gggcttccaa aaacgacttg aacagggcat gcatctcgac
      121 tcgcacggcg atcctcccgc gcccgacagg acgctgatcg acaccatagg ggcatacgcg
      181 gccgccgtca agttcgattt cgccggatcc cggccgcggg aagggggagg aggggcagca
      241 ccctcggaaa gcgattgacg cccgcgaatc cggaccgata caattcccac tcccgtataa
      301 aacctgcccg accccggcgc gggcgagacg ccaggagccg aagtgaacaa gaacgaccgt
      361 ggagcggacc cgaatccccc gatgcccgat ttgccggcga ttggaccgtt ttcgcccgcg
      421 gaccgcaggc tcctcaagcg gtatgaaggg gtcgccgatg ccgtggctac cctcttcggc
      481 cggggctgcg agatcgtact ccactccctg gaggatcttt ctcattcggt cgtcaagatc
      541 gtgaacgggc atgtcacggg caggggcctc ggctctccga tcacggacct gggtctcaag
      601 gtcctgcgct gctcttttga gggcaccgag gacgtgatcg gcagctactt cgccttcacg
      661 gattcgggcc gtccgatcaa gtccgtcaca ctcctgatcc ggaacgaccg cgggatcccc
      721 atcggaatgc tctgcatcaa tttcgacctt tcgacgccgt tggcaagctt catcaaggag
      781 ttctccccgt tccaggatct gccggcgtcc tccgagcact ttcctgcggg tgtgggagac
      841 cttgtccagc acgccctcgc cgaggaagcg gaacacctcg caagggcgaa cggcattccc
      901 atccaggaga agaatcggag aatcgtcgaa ggcctggaaa atcggggctt attcgagatc
      961 aagggagccg tggaatcggt gagcaagggg ctcggcatta ccaaatacac tatatacaaa
     1021 tacttgcggg aattccggaa atcccggctt ccggcggacg gatccggagc aatgaatccc
     1081 ggaacctcgt ggagctctcc ggattagaaa aatattgtca ttcgttaact ttttttattg
     1141 acagcctcca cattcggtcc cagtatacac ctagattctc ccgggaagat ccggcgcatc
     1201 ccgctccgga accggcgggg tcgttaccga agatcgggag gcgaaacatg gcacggacag
     1261 ggaattacat cgacgaacac atcggggaga tccgcaaaga atttaagctg ctggattact
     1321 gggtttacct gaattcggga gaccagatga tcccgggcaa ctactggctc aaggccgcac
     1381 gggaattcta cgacttcgtg gagttcggac ggatggagga catccccgta gcggacatcg
     1441 cgacccaccc gttcctgacg actgcttgga acgactgtat cgaactaggt gcccgattca
     1501 tcaacgcgga cccggaagag gtcaccaaca cctatcgccc agcgatcacc gctaacctcc
     1561 ttttgtacaa ttgcttcgac tggaaagccg gggacaatgt ggtgatcacg gacctcaatt
     1621 acccttccat cgtctatatc ctgatggata tccggaaacg atacggagtc gaacttcggg
     1681 tcgtgaagaa cgtcgggggt gagatactct tggaggacat ggagaaggcg atcgacggcc
     1741 gcacccgtat ggtctgcgtg gacaggaccg cggccttcgg gggattcacc tacgacatga
     1801 aggcgctatg caagatcgcc cacgcgaagg gcgccctggt cttcgacgac gccatccagg
     1861 ccttgggagc gatcaagatc gacgtcaagg acgacgacgt ggacatcctt gtttccggcg
     1921 cctacaagtg gcagtgcgga ccggagggag cgggcctgct ctacgtgaag aaggatctca
     1981 tccgaaagct ggatccgaga taccgcgact atctctgggc cgacatcccg acggggattc
     2041 ccttcgccct gaaggaccac gacaacatgc gccactggga tttcccgccg gcggaaaccg
     2101 ccaacaaatt ctcccaggac gtcacgatcg ggcccgccct cttcggctgg aaggagactc
     2161 tccggttcta cgagaagatc ggaatggaga acgcggagga gaggatcaag cgcctcggca
     2221 cctacctgat cgaacggctg gaggagatcg gctgcgtagt caagacaccc aaggacgtcc
     2281 ggaagcgcca tggcatgatc gtctacacga caggatccta tgaaaccgac ctgaagagct
     2341 tccagcgttt cgccgctccg gggcggtgca tgaagcccgt caaggtttcc atgcgggccc
     2401 tgggcggggt gggaaacatc cgcgcgtgca cgcacttcta caacaccgaa gaggacgtgg
     2461 attacctaat ccgcgtccag tccgcggtca tgaaggccta gggatccgat tcgatccggt
     2521 cccggggtcg gagtacggcg gaagcgtgct ccattcccgg gaccggttta aacaaaaagt
     2581 atcgatttac cgcgtctatc agacgtatag taactgtcgg cagattttta ttgacataat
     2641 gtcaatgagg tctatactaa tcgagattcg gggattccgc gtccccggaa agaggctatc
     2701 atgcggacga aaatcgagac accggatgcc cccgccgcca tcggcccgta ttcccaggca
     2761 atccggtacg gagacctgtt gttcgtctcc ggccagctcc ccatcgatcc cgccaagggt
     2821 gccatggagg ctccggacat cgcggtccag acgagaaggg tcctttccaa cctgacggcc
     2881 gtcgtccgcg ccgcgggcat gaaaccggag aatattctgc gttgtacctg ctttctggcg
     2941 gacatgaatg aattccctaa atttaacgaa gtctatgcgg agtttttcgg caaggacgcc
     3001 ccctcccggg agaccgtcca ggttgctcgg ctgcccaagg acagccgggt cgaaatctca
     3061 gccgtctgcg gggcgtaggc atggttcgcg gaggattcgt tacctacggc aaggttgccg
     3121 ggatcctgat gctcgactcg cggattcccc ggattcccgg agacccggga catgcccaga
     3181 cctttccctt ccccgtagcc tatagggcgg tcgagggact cccgttctcg gatctggtgg
     3241 agggtcggct agacaacgcc gacaaagcta ccgaagccgc agcgagcctg gaggcggacg
     3301 gagtgtcctt cgtcgccgcg gactgcggcc tcttctccgt cctgcagaag aagatcgcgg
     3361 ctaggctgtc gatccccttc ctgggatccg ccctagcctt gatccccctg ctgggaacct
     3421 ttctaccgcc gaaccggtgc gtcggcgtcc taacggggca cacggacctc ctaagggatc
     3481 accaccttcg ggccgcgggg gtctcccccg acgggatcgt cgtggcggga atggagaatt
     3541 cctccgaatt ccggaaggtc gtgatcgatc gggggaccga tctggatccg gaggcgatga
     3601 gagagggcgt gcgggaggcg tcgctggaat tggccggtag ggcatcccgc aagggtctgg
     3661 ggctcggagc cgttgttttg gaatgcacga accttgtcag cttccgggac actgtacaag
     3721 aagcggcagg cgccccggtg tacgacctcg tcagcctggt ggaattgtac gcgtccgggt
     3781 atgcgcggag ggaattcgcc gagacccatc ggtccttcgc gcgcggttag ggtggcctac
     3841 ccggggttgg tcccgggaag agatagagcg gacgttcatt aaggaggctt aaggtatggc
     3901 ggaacagacg aatcgcgaaa ctttcgcgag caaattcggg atgatcatga ccctggtcgg
     3961 cgtttccgtc ggtctgggga acgtctggcg cttcccgtac atggccggga agttcggcgg
     4021 ggccgcgttc gtgtttttct acgtgctcct cttgttcgtc gtgggcattc ccggcctgat
     4081 cgccgagtac aacctgggac gctacaccaa tcgcggaacc ctcggcgcct tccagaaggc
     4141 gggattcccg ggcggcaagg tcgtcggaac ctggttctgg atcgttacga ccttcgctgt
     4201 gggatactac tgcaacgtca tcggctgggt cttgttctac gcggtccggg aggtgggcgt
     4261 cgcggtcggg gcgacggtca atgccgccgc gatactcccg ccggacacgg gcttcaacgc
     4321 gactagcttc ttcctccagt tcttcttcac cggcatcgtc atcctctcct gcgccctggt
     4381 tctggcgaag ggtctcaagg aaggcgtcga aaaggctagt aagtacataa tgcccgcctt
     4441 cttcgccctg ttcctcatcc tcatcgtccg ggccgtgacg cttcccggcg ccggcgccgg
     4501 catcaagtgg tacatcgggg acttccgctg gaaggagctc accggcggat ccatggccgc
     4561 ggccatgggg caggtgatct tctcgctatc cctcggcggc accttcatgg tcgtgtacgg
     4621 atcttacttg aagaaggaca cgaacctggt gcagaccggc atcatgaacg gcatcgggga
     4681 tacgatcgcg gccctcatgg cgggcttcgc gatcttccct gcggtgttcg cgttcaatgt
     4741 cgagccgtcc tcggggcccg ggctcatctt cttcactctt ccgaaggtgt tcgattccat
     4801 gcccgcgggc tggctcttcg cgatcttctt ctacatggcc ctattcggag cggcctatct
     4861 ctccgccatc gcggccttcg aagtcatgat cgccggcatc gtggacaata ccaaactaaa
     4921 ccggaagcag gcgacctggc tcatggctgg cctcggattc ctgttcgcca ttcccccgat
     4981 gatcaatttc cagatcttcg ggccctggga cctcttctgg ggctccggta tgcagaccct
     5041 cgggtgtgtc ctggccgtca tcgcctccgt ctgggtgatc aagcggagcg aaggtctcaa
     5101 ggccctggcg gaagggaccg gcaagcagtt cccgatatgg ctctactggt ggatgcggat
     5161 cgccgtcccc ctcgccgtcg gctttgtcgg cgtgaactgg ctgctttccg aggtcttcaa
     5221 gatcaacatc ttcggttgag tcggatcgtt cgatcgaaat ggatcctccc gccttggcgg
     5281 ggggatcctt tctattcggt gtccagtgct ggacgaaagg gatttcatcg tccgggaccc
     5341 gaccggcccg gtcgggccta ggagtttcgg atctgcgaaa tggcgacacc gaccagcgct
     5401 atccccatac ccaggatctt gacggctccg aacttctccc cgagcacaat agcggagaat
     5461 gcggcggcta tcaccggaac ggcgttcgag aagacgctgg cccgtgccgg tccgagcacc
     5521 cggatcgcgt tgttgtagaa caggtaggaa accgtcgagg gcaggagcgc cagagccccc
     5581 aggctccaat aaagctcggg cgccggaaga atcaagacgg cctctcccgc ctcgtagaac
     5641 aggaagagcg gcaggaacat caaggctccg aaaaagttct ggtatttcac gatcgtgatt
     5701 ccagaaagtc taagggggag tttccgtacc gccacggctc cgagcgaggc cgccagaacc
     5761 gccaggaata ccaggcaggc cccgcccacg gtcgccgagg cgtccgcgag accgttcttg
     5821 atcatgagaa ggaccccggc cagggaaaga atcattcccg cgacggccag gattccgacc
     5881 cgctccgcaa ggaatatccg ggacataagc ggagtcacga ccggaataag cgcgatgatc
     5941 gtagcggcaa ccgccgggga tacgctcatc aggcccaggt tctcccccac aaagtacagg
     6001 aacggcatga acagggagag gatcacgagg cttacaaagt cctttctttc tattcgtgaa
     6061 tgacccggag aggccgtccg gccccggatt ctcagcgcgt ccacgatagc caggaaggcg
     6121 gaagaaagga cgagccggac aaagatcacg gtaaagggcc cgcaatactt gaacgctact
     6181 tttgtccaaa gaaaggacaa gccatagaac aacatggcaa agcagtttcc cgcgattgcc
     6241 gccgccgtcc gcttttcatc tgccattgag cacctcgttg cattacctgg ggagtcttct
     6301 tcgtttcgat ggaaatgatg ccgggatatg gacattatgt aaaggggaac tggttgaaac
     6361 gcatgctgaa ggtagctcca agctgactac ttctggtaat cgcgccgctg accttcttgt
     6421 ccctgtagtg gaccgacccg gggttaacga tatattgtta agtgagttca tccctttgta
     6481 ccatacgagc ttcatatcat gcttactatt ctcaagctcg ttagtaagga tgttcccgag
     6541 cgatcggaat ggttttggca ttatcgccta cgccgataga cataaggatc ccgacttcag
     6601 ccagccattt ttatcagaca ctggggtttc ttgaaggata ccattccgtc aatacagacg
     6661 gcctccttcg gatcggagaa tcgacgcaga tgggcatgaa gggcgatgcc ctggcgggcc
     6721 aaccggtcca ggcgattctg gaccgtaccg caggatagct tctggtttcg gctcagggct
     6781 cggaggcttt cggaggaggc atggcggtcg aggagtccgg ggtaatctac cgttcgtttg
     6841 gcgtagtaat cgatggaaaa ggactgtgtg gagaaggtct tgccacagga tcggcacctg
     6901 aaccggggga cgaggccgaa ggtacgggtg ggatagaaac cgaaggggga gaaccaaccc
     6961 ggaggaggtt cgcttaaatg caggtcacaa aaaggattgg gacagaaggg gaacgagggc
     7021 ataggtacct cctggaggct ggaaacctcc cgtccctatt ccgctcgatt cctcttcctc
     7081 gcttgcgtca tcacaaggtt cgtaacacta tgcaggaact gcggccggtc ggtccgggga
     7141 tggtcgatcc gtatgagccc cgcccggtac gcaccctcga ccgcatcctc ggagagggcc
     7201 tcccgggggg cggccggggg acggagccgg cccccggtca aaacaccgat ccgatccgcc
     7261 cagagggagg ccaggttgag gtcgtggatg gtagccgcca ccgccgtacc ggaccgggcc
     7321 aggcgccgga gcatgcccag gatctcggct tggtgccccg cgtccaggtg ggcctccggc
     7381 tcgtccagga ggaggaccgg ggcttcctgg gccagggccc gggcgatcaa caggcgccgg
     7441 aactccccgc ccgagagctc cgaagcggga cgctcggcgt cccccccgag gcccacggcc
     7501 gccagggcgc cggcgacggc gcgctcgtcc tccggcgttc ccggggcgaa ccagcccagg
     7561 cgggcaaagc gtccggccca gacggtctgg cggacggtgt atccgggcac cggggcctcc
     7621 atctggggca cgtaggccgc cagccgagcc cggtcccgga tccccaggcg ggcgatcggc
     7681 cgcccccgca cgaggatctc cccggaaaga ggggccaaaa ggcctcccaa gcttcgtaac
     7741 agtgtggtct tgccggaccc gttcggtccc accagcaccg tgagttcccc ctccgggatg
     7801 tccaggtcca cgtcccggag cacgggtaca cgccggtacc cgcaggccag gccccgggcg
     7861 gacagcgcgg tcgggttcac cgccgtcccc cgtcccggcg ggccaggatg accaggaaga
     7921 agggcgcccc ggcgaaggcc gtgagcaccc cgacggggag ttccaggggc gccagcacgg
     7981 tccgggccag ggcgtccgcg gacatcagca ggacggcccc cgcgacggcg gaggcgggga
     8041 tcagtcgggc gtgagagggt ccgaacacca gccgggccgc gtggggagcc accaggccta
     8101 cgaaccccac gaccccgccc gcggccacgg ccgccgcggt gcccagggcc gccagggcca
     8161 cggcggcggc ccggaggagc cgtacgtcca gacccaggga ccgggcgctc tcctcccccg
     8221 cggccaggac gtccaggggc cgtgccaaca gcagggccgt cccggagacc agggccgtca
     8281 ggggggccag gacggccagc tccttccagc cccgaccgtt gaagctcccc aggagccaga
     8341 agtacacggc atacaggtcc tgctggcgga ggctcaccag gagggtcacc ccggcggagc
     8401 agagggcgct gacggcggtc cccgccagaa ggacggcggt ggtggagccc gagcgggagg
     8461 tggcccgggc cgccgcgccg gacagggccg cggcccccag ggccccggcg aaggctccca
     8521 gggacgcgga gccgggtgtc cggacgaaga ccagggccgc ggccgcgccc agggcggctc
     8581 ccgcggaggt gcccaggatg tagggatccg ccaggggatt ccggaagagc ccctgcagga
     8641 ggaccccgga caccgccagg gcggccccgg ccaagcccgc ggccagggtc cgggcaagac
     8701 ggaggtcccg aaggatgacc gcggccacgg gatcggcccc ctgcttccca agcaggacgg
     8761 ccgcgacctc ccggggtccg taggaaaccg cacccatccc gagggatgcc aggaggacga
     8821 ggaccagcag gcccagggcc gccagggcgt aggcgagccg gacgacggga cgccgtgcgg
     8881 cacgggacgg aacggaagcc gtccggaggg accgtgcctt cacgggaaga gctccgggta
     8941 gagggctcgg gcgatggacc ggaccccctc cccgagacgg ggacccgtcc ggttcaccgg
     9001 gtccgccggc agcatgtaca cccggccggt ccggaccgcg ggcacggtcc cccagcccgg
     9061 ccttcgggcc aggtccccgg cggtgaagcc gggggcgtgg tcgtccgcgg ccaggatgac
     9121 ctggggcgcc ctccgcaccg cctcctcggg ggacacgatc gcccagggct gggtcatctc
     9181 ggcgaagacg ttgatccctc ccgcctgggt gatgagctcg tgcaggaagg acggggcgcc
     9241 gcaggtcatc aggggctcgt tgtagacttc ccagaagacc cggacccgcg aggcggctgg
     9301 aatcgaggcc acccgggcgg tgacctcggc caggtcccgc cgtaggccgg cgatcgatcg
     9361 cgcggccccc tcggccgtgc ccgtcaggac ccccagatcg gccaggcact ccagggtctg
     9421 ctcgaccgag gaaggctcga agacgtaata ggaaatcccc agggagtcca gggacttgcg
     9481 cagggagacg tggaccggtc ccccggtcac gaccaggtcg ggcttcaggg tcaggaggcg
     9541 ctccagggag atggactcgg aggtgtagcc cccgacccgg gggacccgcg cggcctcggc
     9601 gggccaggtg cagtaggagg tcaccccggc cacccggtcc ccggccccga tcccgaaaag
     9661 gctctccgtg gcgctggggt tcagggagac cacccggcgg gcggggccgg acagggtcac
     9721 ggtccgctcc aaggagtccc gggccgagag ggtctgtccc caggctacgg aggccaggca
     9781 tgtcaggccg gccagcagga tggcgacccc gtgggtgctt cggctcatgt cttcttccct
     9841 tccggcgctc cgggcacccc ggcttcctcg aaggtggcca tccgggtgtg gacctcgcag
     9901 gcggcccggc agaccgcggc ggccaggaca gcccccgtgc cctcccccag gcgcatcccc
     9961 aggtccagca ccggcgtcag gcccaaaagc ccgagggccg cggcctgtcc gggctccacg
    10021 gaccggtgac cggcgatcat gtactccgcg gcgaggggac agagcccgca ggcgatcatg
    10081 gcggccgcgg cggagatgaa cccgtccacc aggaccggga tcctccgagc cgccgcggcc
    10141 aggaagaccc cggccatggc cccgatctcg aagccgccga ccttggaaag gacatccagg
    10201 gggtccgagg gatccggccg gttcacggcc agggcccgtt ccaccgcggc caccttccgc
    10261 tccaggcccc ggtcgtcgac cccggtgcca cggccggtca cctcccgggg cggccggccc
    10321 gtcagggccg cgacgatcgc ggaggcgggt gtcgtgtttc cgatgcccat ctccccggcg
    10381 gcagccaggc cgaagggtcc gaactcgaac tcctcctcga aggcctcgat cccgatctcc
    10441 acggaccggc gggcctcgtc ccgggacatg gcgggccccc gtgtgatgtc gtccgtcccg
    10501 gggcgcaccc tgcggatccg caggcccgtg gcgggaccca ggtccgaggc gaccccgcag
    10561 tccgtgacgg agacccggaa gcccgcgaaa gcggccaggg cgtttaccgc ggccccgccg
    10621 gccaggaagt tgcggaccat ggcggccgtc acctcccgag ggtaggcgct gacccccgcg
    10681 gcggccaccc cgtggtccgc ggccatcacg aacgcgacgc ccggcccgag ctcggggacg
    10741 gaattccctg cgatgcccgc gatccggacc gcgaggtcct ccaggcggcc caggcttccg
    10801 gaaggcttgg tcaggcgggc ctggcgggac cgcgccgcct cggccgcggc ggcatccggc
    10861 agtccgatac ggctcagggt tgtttcgagg aggctcgtcg aagagcggga ggataaggag
    10921 gttctcgaca tgatccgtca cccagcggat tacgaggtcc cggaaccagg tcccggggac
    10981 tcaggcaggt ctcctgactc cgaggctccg gacgttccgc ggagggatcc gcgtccggcc
    11041 ggagtccggg accttcccgc tacacgcagt ggttcccggt atctcgcctc gttacagtgc
    11101 ctgggggcgc gccggtattc caccggcttc ccaattaagc ctttcggcgc ctgcgccgga
    11161 cccaccgtac accccccagg cgtccggggt caaggagagg ggccgtatcc gcccgcgccc
    11221 cggaacgggt ccgaacgccc ggtcctccgt ccggcttgac gaacgccgtc cgggggcgga
    11281 gactgaaggc atgaagaacc aggcccggcc cgtccgcgcc gcggggctcc tcctggcggt
    11341 cctcctggcc gccgcccccg tcacggcctc ggacggcttc gagcgggaac gcttcctcat
    11401 cctcaactcc taccatcacg gcttcgagtg gtccgacggc atcctccggg gcatcctgga
    11461 ggtcctgagc cgggagttcg cgcggccggc cctgcacatc gagtacatgg acacgaagag
    11521 gcatttcgac ggtatcgacg gtccctacct cgacgggctg gccgccgtgt acgccgccaa
    11581 gtacacccgg accaagttcg acgccgtcct gtgcaccgac gacgcggcct tcctgttcct
    11641 cctgcgctgg ggtcggacgc tcttccccgg gacgcccgtg gtgttcacgg gggtgaacaa
    11701 cttcagcccg gacctggtgg ccggccggcc cgagatcacg ggcatcgtgg aaaggacgga
    11761 gtacggtccg agcgtcgagc tggggctccg gctccggccg gaggcccgca ggctcggggt
    11821 catcacggac gcgtccctga acgggttggc caaccgcgcg aagttcgagg ccctggcgga
    11881 atccttccgg ggccgcctgg agttcgtctt cttccggacc gacggtcttt ccgaactcct
    11941 ggaggaggtg cgggccttcg accccggggg actcatctac ttctcggact tctacctgga
    12001 cggccgggga cggtcctacg aatacgacgt ctcggtgccc gaggttctgg ccgcggctcc
    12061 ctgcccggtc ctctaccacg cggacatgta cctccccttc ggcgtcctgg gcggccggat
    12121 gaaccggagc cgggagtacg gcgagctggc cgcccgcatg gccgcccgca tcgtccgggg
    12181 cgaacccccc tcggccatcc cggtcatgca ggattccatt gcccgggcgg tggtcagcta
    12241 ccccgccctc cggaaattcg gcatccccga gtcccgggtc ccccccggca ccctcgtggc
    12301 ggacaagccg gaaagcttcc tccggcgcaa cgcccgtctc ctgacggttt cggggatcat
    12361 caccttaagc ctttccgggg tgatcgtctc cctcctgtac gcgatccggg aacggaggcg
    12421 ggcggaacgg gacctccacg cctcccggga gaccatcctc caaagcctcc gggagaagga
    12481 ggtgctgctc caggaggtcc accaccgggt caagaacaac ctccagatcg tgaccagcct
    12541 gctgcggctc caatccgcgc actccgggaa gccggaagtg gaggaggcgc tcaccgaaag
    12601 caccgaacgg ctcatggcca tggcgctggt gcacgaacag ctctacctct ccaagaactt
    12661 cgcggacatc gaatactccg agtacgcggc ggggctcgtc tcctccctgc gcgccacctt
    12721 cggactctcg gaggaggcat tccggtgttc catcgattcc agcggcctcc gcctgggcct
    12781 ggacaccgcc gttcccctgg gcttgatcct gaacgaactg ctcaccaact cggtgaaata
    12841 cgccctgccc gcccgctccc ccctctccgt ccgctccctg ctggaggaag cggacggcat
    12901 gctgaggttc cggtaccagg acgacggacc ggggcttccg gtcggactgg atccggaaaa
    12961 ggacggcggc ctggggctca ccctgatcac cgcgctgagc cagcagctcc ggggagcccc
    13021 cgcgttcccg cgggatcggg ccggcatggt cttcgagctg gacttcaccc cccggttccc
    13081 gccccgggga aaaatctgaa cggcaagccc cgcccgacat cttgcgtccg ccccggcttg
    13141 ggattaggat ttccttgaac ggaccctcct cggccccggg tgtcggggac ggtccggaat
    13201 ccgatttccg ggaaggccga tgttcgcgag attcggagcg atcgtttcca tagccgcctc
    13261 atccttcgcc ctggcggtga tgatggtttc cgcggccctg cgctcccgga cgagaggccg
    13321 gaccctatcc ttcgcggggc tttgcgcctc cgccctggtc tactccctgg ggtacctgct
    13381 cgaactgctg gccccggatc tggaggccgc gctccgggtg ctccgcttcg aatacatcgg
    13441 gatcgtcacg atagctccct tcctgaccct ggtggcctac gagttcctgg acgagggggc
    13501 gccgagggtg cccgctcccc ttttgttcgt tatccccgcg gggatcctgg ccctggcctg
    13561 gacgacccaa ctccacaggc tgtactacct ggacccggtc ttgacccggg tcgcgggcct
    13621 gtcggtcctg gaattcggcc ggggtcccgc ctactgggtc cacgtgctct accagaatct
    13681 cctcatcgcc tactgcgtga ccatcttcct ccgcaacact ttccggggtt cccgggcccg
    13741 gcgcgtccag gcccggttca tgctggcggg atgcgtcctg ccctggggcg gcttcgtggc
    13801 gtacctggcc ggcggggttc ccctgaacct ggatcccacc ccggtcaccc tggccttcac
    13861 ggggatcttc ttcgcggtcg gcttcttccg gtacaagatg ttcgatctcc gcccgatcgc
    13921 ccgggacacg gtcttcgagc agatgaggga cgccgtcctg gtcaccgacg atcagggagc
    13981 cgtcgtggat cacaacaccg cggcccgcag gctcttcccc gccctggcga gtttccgcgg
    14041 tatacgaacc gcccgggacc tggcccggga cgccccggac ctcgccgaag ccatgtcccg
    14101 gtcggcatcc gactccgtcg tgaccctggg cctgcccgag ggggatcgga gcttcaacct
    14161 ccaccattcc gccctctccg accggggcgg ccggagggtc ggcacagcga acgtgctcac
    14221 ggacatcacc gagcggctcc tcctggagga ccggttgacc gtccaggcca ccacggacga
    14281 gctgaccgga gtggccaacc gtcgccattt cttcgaccgg gcccgggtcg agctggagcg
    14341 ggcccagcgg tacggacgcc ccttcggcgt ggccatcctg gacatggacg atttcaagtc
    14401 catcaacgac aacttcggac atcccgcggg ggacgaggcc ctccggatcg cgtcccggct
    14461 ctgcgcggag accctgaggt ccacggacat catgggccgc tacggggggg acgagttcgc
    14521 cttcgccttc ccggaatgcg acgaggacca ggcccgggaa gccgcggagc gcctgtcccg
    14581 gatcctggct tccgcggcct ttccctacgg ggatacggtg atccggctct ccgccagcgt
    14641 ggggtacgcc gggggttccg agccccccct gccggacctg gattccctcc tgaagctcgc
    14701 ggacgagcgg atgtacgaga aaaaaggccg gccgccctcc ttcaagtccg tagcccggga
    14761 cccggaccgg gactaggccg cggaccggct gccgccgatc cgcgggcaat gagcttcacc
    14821 cgtcgagttc tccggatcgt ctcgcggccc tgcgcttgac ccgccgcccc ggccgtggta
    14881 cagcctaggc atgcgaaccc tgatcatcgg cggcgtcaag tccggcaaga gccgccgcgc
    14941 cctggaactc gcccgggcct tccccgcccc ccggaccttc gtggccaccg cccaagcctt
    15001 cgacccggag atggccgaaa agatcgcccg gcaccggtcc gagcggggag acgacttcga
    15061 aacggtggag gagcccctcc ggatcgaccg ggcggtacgg gagccctgcg tcctggactg
    15121 cgtacccctc tggctcaaca acctcttcta cgagaatcgg gagtccgagg ccgaggggat
    15181 cctggactcc ctcctggaga ggctacccgc ctcgatcgtc ctcgtgacca acgagatcgg
    15241 catgggaacc atccccatgg atcccctgtc gcggcgctac ggagaggccc tgggcaggat
    15301 caacgcccgc ctggccgcgg cggtcgaccg ggtggagttg atggtcgcgg gcatccccct
    15361 gcgcgtgaag tgacatgatc ctctccgtcc cctcctacgt gattccgggc acctacgcgg
    15421 agaacctccg cttcctggcg gacaagccgg aggtggaggg ggtggagctt ctcttctacc
    15481 tctgggatgc cgaggtcgcg ggtctgctgg atcgggagct tgccgaaatt cggtcgttca
    15541 tggaccggtt ccggttcacc gcccacctgc cggaccggat cctgccggag catcgggtcc
    15601 tcctggaccg gatcgcggac acggcccgat ccttcgtggt ccatccgcct tcggaccttg
    15661 acgggctggc ccccttcgcc gatcttttaa gtgtctggaa aagcgggttc ggagaccggt
    15721 tcttcctgga gaatcttcga tccgaaccct tcgggcgtgc gctccgggtt ctccccgaca
    15781 ttccgctctg cgtcgacgcc ggccacatcc tcgccgaggg cggcgatccc gcggaactgc
    15841 tagcccggct tcgttcgacc gggcggcgga tcgaggagct tcacctgcac ggtctttccg
    15901 gggacgccga ccaccagccc ttcggcgagg gggcttcctg gctccgggac ctggcaccct
    15961 tcctggccgg gttcgacgga gtggccgaga tcgagttgtt cgactgggag gaagcccgaa
    16021 ccgccctggc cgcggtacga cgggccggtg tcgaggcggc cgcctcggaa gccgcggccg
    16081 gaccccggag cggccgtacc gcacgcgccg ccgcgccggg caaggagacg gaatgagttc
    16141 gcccctggat cccttcctca gcgtcctggc cctgacctgc cggatccggg tgcccgcgcc
    16201 ggcggagccg gactacggcc gggcggactt cttcttgccc ctgacgggcg tccttcccgg
    16261 gctggcggcc ctggggggct ggggcctcgg gtccctcgtg ttcccgggcc cctggctgcc
    16321 cgccctttcc gccctggcgg cccagtacct ggccttcaac ctcttccacc tggacggcct
    16381 ggcggactcc gcggacgcgg gcctggccct ggcgagcccc gaacggcgcc ttgcgatcct
    16441 gaaggatccc cgggtcgggg tctacgggct cttcgcggcc ttcctggtcc tggccgcgcg
    16501 aacggcgggc ttggcagccc tgggcggtcg tccgggcttc cccgcggtcc tcctggccgc
    16561 ccccgtggcg ggtcgcttcg ccgcggccct ggtacccctg acagcccggc ccgcccggcc
    16621 cgacgggctg ggggcgagga tgcgggactt ccgtccgcag gccgtcgcct cgggcctcct
    16681 ggccggcctg gccccggcct tcctggccgt cctggcaggg cttcccggtg agggatccgt
    16741 gctcgcgggc atcctgggta ccggcgccgg agccctggcc gcgggactgg ccgcgggact
    16801 cgggacggcg atcttctacc gccgcaggtt cgggggcttc accggggacg ccctgggcgc
    16861 cgccgtggag ctcggggagc tggcctttct cctgggcgcc tgcgccgtcg cggggacggc
    16921 cggaggcgcc tgacatggag ctcctggact tccggtccaa cctgaatccc gagggacctc
    16981 ccccctggct ggccgaggcg gaggaagccg cccgaaccca gtccgccccg gccgtgtccg
    17041 gattccgtaa ggccaaggag gcctgtttcc gaaggttttc cttctccccg gactgcttcg
    17101 tcttcgggga aggggtcaac ggccttctgt ccgtgctgcc ccgggccctc ggcgtggacg
    17161 ccgtcctgat cctgcccccg acctactccg gattccagcg ggccgcggcc gccgcgggtc
    17221 tgcccctcca cctccacgcg gcccgggaag aggacggcta caccctccga cccggaagcc
    17281 tccgggcgga catggcccgg atatccggtc gggccctcgt cttcctgggc cgcccgaaca
    17341 acccggccgg cgtacccctt ccgatcgaga ccgtcatcga ggccgcccgc gtcttgccgg
    17401 gccacttcct gtgcgtcgac gaatcctacg tggagctgtc cgagggagtg gaacccgtcc
    17461 ttgcccgggg acgccgcctc ccggacaacc tcctggtcct ccgctccctg accaaggccc
    17521 tggctatccc cgatctgcat ctgggctttg tcggggcgtc ccggaccgtg acccgcagga
    17581 tcgaggacgc cctggagctt cggccgagga gtcctcgggc cgaggagatc gccttccggg
    17641 cggtctcgga tccgcggttc ttcgagacct ccgcggcccg gatcgccgga taccgggccc
    17701 ggttcgcgga acggctgtcc cgaattccgg gggtgaagcc cgagaccggg ttcgccaact
    17761 tcctgctggt ccgtatccag ggggcggacg cccggaaggc cgccgcggcc gcgcgggaac
    17821 ggggcgtcga cttccgggac tatgcggagg atgactacct ggacgggagg cgctggctcc
    17881 gtacggccgt ccggaaggac gaggacaacg aaaaggccgc gaccgccctg gaggcggccc
    17941 tccgggaggt ccgggaagcc gggaaggcct aaccccgcat ccgggcggcg gccagctcca
    18001 cggcgtccac gatctgctgg taccccgtgc agcggcagag gtttcccgaa agggcggtcc
    18061 ggatggccgt gcgg
//
LOCUS       DAOTEI010000062        17582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2172_length_17582_cov_5.370457, whole genome shotgun sequence.
ACCESSION   DAOTEI010000062 DAOTEI010000000
VERSION     DAOTEI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 17582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17582
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2172_length_17582_cov_5.370457"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="P5313_07830"
     CDS             complement(<1..308)
                     /locus_tag="P5313_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRY80309.1"
                     /translation="MKTLIAYSTRWGMTERTARLIAETLKEADGRPTHVLDARKVGRR
                     ELETYDAFVVGSSVAAGRWKGPARRLLARLAAARKPTAVFVTAAGVLSGKEPGSAKLK
                     "
     gene            complement(408..1334)
                     /locus_tag="P5313_07835"
     CDS             complement(408..1334)
                     /locus_tag="P5313_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY80310.1"
                     /translation="MKNRIALLLILALALPLALFADGTKEKAVNPDVIQVQLIPSRDA
                     AYLDAQRMPMQQMLEAQLKRKVNVTVATDYNALIEGMAAGQIHVGLLATTAYVMAADE
                     GFAEAILKSTRYDVDDNGNLLKDKPLVSGYKAQLVAGADSGIKSVKDLKGKKIAIASF
                     TSTSGFVWPANLLADNGLDPEKDVTWMNVGGHDNAITAVFNGQADAAFTFKDARTLFS
                     KEAFYPDLMKKVVLISNTSEIPNDTISVIPNLDPKLKEAIRMAFINMVKDPKGLEIMR
                     KIYNHEGYAPAKDSDYDLVRTYLARQKKWTFK"
     gene            1510..2292
                     /gene="phnC"
                     /locus_tag="P5313_07840"
     CDS             1510..2292
                     /gene="phnC"
                     /locus_tag="P5313_07840"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HRY80311.1"
                     /translation="MIQFTNVSKTYPNGVQALKGVNLSIDKGEFVAILGLSGAGKSTL
                     LRLINQLIDTTEGEVTVNGVAVNRATGKTLRNVRRQIGMIFQGFNIVKRMSVLSNVLS
                     GRVAYNPTWRTVLGLFPEEDKQIAYRALKRVDILEKVHTRASELSGGQQQRVAIARAL
                     AQEPLVMLADEPVASLDPVTTIQVMNYLKKINREDGITTVANLHHVDLALKYATRVVG
                     IRAGTIVYDKQMKDIDKDNFLHEMEQVYGRRIHSDEVLGDEE"
     gene            2289..3083
                     /gene="phnE"
                     /locus_tag="P5313_07845"
     CDS             2289..3083
                     /gene="phnE"
                     /locus_tag="P5313_07845"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRY80312.1"
                     /translation="MSGPAERIVVPKPNHLRRNLIILGVTALIVGSAWFAGFDLGRIL
                     LNLRKGGRLLGRMFLRPDWAYTPRIVDPLLETVKMSVVGTFWGAILAFPTAVFAANNF
                     IKNPWIAQPLRVILNVTRSIPAMVMASVFVAVFGIGAFSGVVALVIFTFGLISKLTFE
                     SIEAIDPGQVEALQAVGANKPNVLRYAIVPQILPQFLSYTLYGFEINVRAAAVLGYVG
                     AGGIGQIFEQNLAWRNFDRVGLIIIISFFVVLVIDLASSAMRRKLV"
     gene            3080..3883
                     /gene="phnE"
                     /locus_tag="P5313_07850"
     CDS             3080..3883
                     /gene="phnE"
                     /locus_tag="P5313_07850"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRY80313.1"
                     /translation="MTDRILVRREPNPLRTLYNILNYGGLAALVLWSSSGIEYLGIMQ
                     TARGTVEALLKGFVLPDTAYLANWTKDGLPYAIVETIAISIAGTFASALLSIPVALLA
                     SQNIVGPRVSKLGKVIVTAVRTFPELILALIFISVVGPGAPAGILAIGIHSVGMLAKL
                     FSEAIESMDMGVKEALDSCGATPMEVLFRAMLPEVAPEFISYTLYRFEINVRAASTLG
                     LVGAGGIGAPLIFAIRNRAWPRVGIIMIVMVVVVTVIDLVSGRLRKKLV"
     gene            3888..4634
                     /gene="surE"
                     /locus_tag="P5313_07855"
     CDS             3888..4634
                     /gene="surE"
                     /locus_tag="P5313_07855"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRY80314.1"
                     /translation="MNILLVNDDGVEARGLALLARVMREHGELYVVAPHSQQSAIGHG
                     ITIHDPIRVHEREGLFPGSGARVWSLEAKPADCVKFALFALDLPVDLVVSGVNDGPNM
                     GTDIVYSGTLAGASEAVICGKPAIAVSTDFGYFDLAERELAGVMSTILASGILSKDNV
                     LNVNFPRRGFEASLGMKVCDMGDRPFLHEFVEIDGVYWAKGTWGEGNNPPGTDVWAYK
                     NGYVSVTPIQVNRTNYEYMKVLRERLPAAE"
     gene            4650..5891
                     /locus_tag="P5313_07860"
     CDS             4650..5891
                     /locus_tag="P5313_07860"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRY80315.1"
                     /translation="MDGIWQRLGITFTEPYRDPLWGTILFPSEFLDILSSPEFVKLSR
                     ILQLGPTHLVYPGATHTRRAHSIGVFSMAARVLKAVAPGAGDLVTPEGARAFLAAALC
                     HDVGHFPYAHSLKELPLEEHEALAARALSGPMAAALERAGADPAQAASIVDVKSPAVG
                     TGTALYRSLLSGVLDPDKLDYLNRDAWACGVPYGVQDTEYMLRHIVLDEEGRPGVDDR
                     GVMAVESVLFSKYQMYRAVYWHKGVRAPTAMVKKAVIEALRNGSVDSADLYGLDDAGF
                     YALMAAPGRDPEGLVRSVFDGRIFPSCYEAPYDEGVPAQRKAAHLVTRTAVEKELAAA
                     VSARTGRPCRLVVDLPEPLSFETSMNVAGTGKKFSESATVFRPEVVRGFSHSLRVLRV
                     FCDADPGAAAPAVAEILTGRA"
     gene            complement(5888..7141)
                     /locus_tag="P5313_07865"
     CDS             complement(5888..7141)
                     /locus_tag="P5313_07865"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRY80316.1"
                     /translation="MGAKVVLAYSGGLDTSVILQWLLNKGYEVICFLANVGQQEDFEK
                     ARQKALKIGASKVYLEDLREEFVTGYIFQALKANALYEGRYLLGTSLARPVIAKHQVR
                     IAHEEGAEYVAHGATGKGNDQVRFELTYAALGPELKVISPWKDSEYLAAFQGRSDMLK
                     YAEDHGIPVSATVKKPYSEDENLMHISHEAGILEDPASPAAEDIYSRTVSPKEAPDVE
                     TRIDIEFKDGLPVRVSRQGTKESVTGALAIFEYLNDLGRANGIGRVDMVENRFVGIKS
                     RGIYESPGATILWAAHRDIEGIAMDKEVMHLKLMLEPKFSEYLYNGFWFAPEMDFLMA
                     AFNQSQEAIDGTVTMSLYKGNAMAVARTSPTSLYNQELGSMDVAGGFDQTDSRGFIRL
                     NSIRLKAHNVILDKRHVGLFPKIQP"
     gene            complement(7312..8088)
                     /locus_tag="P5313_07870"
     CDS             complement(7312..8088)
                     /locus_tag="P5313_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRY80317.1"
                     /translation="MAENSETVQAVEKAIRVLEYLAEHGDCGVSDLARAIGGQKSTVF
                     RLLSTLKEAGYILQDGETEKYCLSLKLFRIGSGAIRNLDLNKAALPVIGRLAKMTSET
                     VHLCILDNDQVLYLHKIESTFSLKVAMVSRVGFVTPLYCTGVGKVLLAWQEDEYIRRY
                     LKEAKLERFTEHTITDPLALAAELQRIRMSGFAYDDEEHELGVRCAAAPIFDLHGRIA
                     AALSVSGPSVRLSDRQMESVRDLVKDSAREISRRLGCETA"
     gene            8288..8941
                     /gene="eda"
                     /locus_tag="P5313_07875"
     CDS             8288..8941
                     /gene="eda"
                     /locus_tag="P5313_07875"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRY80318.1"
                     /translation="MSDPAAFREQIRRSPVVAVLVIDDALDAVPLARALVRGGILAME
                     LTLRTPAAFDALRRIRSEVPELYAGVGTILFPDQVARVKASGAAYGVSPGFNPAVVDA
                     AREAGLPFAPGVATPSEIEAAYGKGCDVVKVFPAEPLGGLPYIKAIAAPYSHLGIAYI
                     PLGGVSMKNLESYLEWPEVLAVGGSWLATRDLIKAKDWDAISRNCEEAASLAARIRG"
     gene            8945..10039
                     /locus_tag="P5313_07880"
     CDS             8945..10039
                     /locus_tag="P5313_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRY80319.1"
                     /translation="MAKFVTFGEIMMRLKTEDFLRFRQALPGRLEATFAGAEANVAAS
                     LALLGAEASFVTTLPAHPIADACLGSLRNLGVDTGGVLRSGKGRLGIYFVEAGANQRP
                     SNVIYDRDGSSVARADPASYDWDAIFRGASWFHWTGITPALSREATEAVRRAVHRAKD
                     LGLTVSCDLNFRKKLWAWEAGTSPNALASRVMRELLPFVDILVANEEDAKDVLGIDVG
                     SLDPSRGTLDAGRYPEVARGIVEQFPNIRKVAVTLRESVSATHNDWGGMLYDAASGSA
                     YFAPLEDGEYRPYPIRSIVDRIGGGDSFSAALILALTDPELGGSDADAVAFATAASCL
                     CHSIHGDFNFSTREEVRALTKGRTSGRIVR"
     gene            10107..11078
                     /locus_tag="P5313_07885"
     CDS             10107..11078
                     /locus_tag="P5313_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY80320.1"
                     /translation="MKRTAWNLAGRALAAVVLALVALAPVAAQAFPRKPVTVICPWAP
                     GGGTDTILRALCKEAEKTLKQTINVTNNTGGGGAVGHSAIMQAPADGYTVGMITFELN
                     SLPPQGLIPFDYKAYDPLMRINADAAALTVHKDAPWNTLKEFIDFAKANPGRVTVGNS
                     GPGSVWHIAAGLMAQKTGTNLVYVPFDGAAPAVTALVGNHIRAVSVSVAEVRGQVQAG
                     QLKILGVMDTQRNALFPEVPTFREQGVDVVFHTWRGLALPKGVPPAVKKTLSEAFKAA
                     FDSPDFKAFAEKASLALAYQDADEFGKFLAQNAADVAAVMKSLGLAK"
     gene            11116..11583
                     /locus_tag="P5313_07890"
     CDS             11116..11583
                     /locus_tag="P5313_07890"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY80321.1"
                     /translation="MRIHDIAGGAVGAGIGLYALIQGAKMPPDHIMKIGPSFFPSLVA
                     AVLIGFSALLALQGALSRKPGGFERVDFRSFGIWRALLSLGAAAAYTALLRPVGFLPM
                     TVLFILALMLLLGARGPLGLVLVPLAATAGVWLVFEQVLLITLPVGLLGLLGF"
     gene            11593..13113
                     /locus_tag="P5313_07895"
     CDS             11593..13113
                     /locus_tag="P5313_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY80322.1"
                     /translation="MDFGLFLTSLGNVVLHPSVLLMVFIGVAGGITIGALPGLTATMG
                     VALLVPFTFGRPPTESLAMLLGIYCGAMYGGSISAILIRTPGTPAAAATVLEGYPMGQ
                     KGQAGRALSMALFASFCGGTIGALIMTFLSPVVSRFALEFGPVEYFALALFGLSVIIS
                     ISGDSVVKGVMSGMFGLLLATIGFDPISGFPRYTFGRVELLEGPAFIPTLIGLFAVSE
                     ILNSIEKMGVLKEIKASIDRYIPSWQDIKVSLKNIVKSSLIGTAIGSIPGAGGDIAAF
                     VSYGEAKRSSKHPHLYGTGVIEGVAATEAANNACSGGAMIPLLSLGVPGDAVTAVLLG
                     AFVIQGLQPGPLLYREHLDVVYNVFASMLVANVAMLLVGMAGIRFFARIILVKREILN
                     PVIFVLSLVGSFAMRQNIFDVGLTLAFGVLGWIMMRAKFPLSPILLALILGPMTEANL
                     RRALTISQGDYSILVSRPIAIALFVLAALSLVVGILNQRKIRKLLAESVEKGQEPG"
     gene            complement(13182..15320)
                     /locus_tag="P5313_07900"
     CDS             complement(13182..15320)
                     /locus_tag="P5313_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80323.1"
                     /translation="MPESGPAVHLAFRFHVNFYHSYRGDSLDERGIGKDIRIIRGILA
                     DLDRLNSEGIPVRGTWDIENYYSLETLMPRHAPDLVEGIARRVREDRDEIEPMSWNNG
                     IVSACTAEEFEELARLAVSNPGRSGLADVFGRWVPILRPQECMYTPSFLSRYPAAGIP
                     AISLYYSAVPFNGFSNFVRPLAFPERYNPLTLESSSLAGSMTLLPCYNHGDVADRGAS
                     LRLWLKGLRRRQLRMEEPRDLLLILDADADDEFWAGMKIPVVSRLLPSFDGLARMVRS
                     VADLPFLRFTRPGDYLRDHPPVGRVVLDQDTADGSFDGLSSWAEKWENTELWTVIQRT
                     RRLYDAAVAAGAPTDDPRLAESLRERLLALSTTHFGMASPVMHAGRLEDGFARARRAG
                     ELAAAVLAERRGVSAGAKAAPESRFPPPAPAPDSAPASGRSGRAEGRAGDGGRFRVLD
                     PRITWGGRVRAGTVEEVGDETRPDGTAVRTLRGALSLPGRIRDGRWTRIETRLAGDDA
                     VFLSLELEYPETPHRGYDKAKALRLDRTWDHRWAEVLPAEILPLFDADPARPFRVIKR
                     NFFGDVSAYPVDHHLVSGSRRTASFNNHIAHPWVAVSNGSEGLLVAQYEGDRCNFAFC
                     PMRSEVSGGRQRLRLNPFGTYYGPQWKYATAVTGLGRAMAVLMADQLDSYAPSYNGKT
                     SRFSFAVFPFRGPEPPEEIRRRAEAWGEVR"
     gene            complement(15313..16794)
                     /locus_tag="P5313_07905"
     CDS             complement(15313..16794)
                     /locus_tag="P5313_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HRY80324.1"
                     /translation="MNQERKPLGLSVRIGYGMGDIYGGGSTTILNMYYLYFLTDMAGL
                     SPIWAGTAILVSKLWDAVTDPVMGLLSDNTRTPLGRRRPYFLAGIPLVFLSFAAMWVR
                     PGFAGPGAAFAYYLTAYVLFSTVYTMVWVPYNSIAAELTEDYDERTRLSFYRMVFSNL
                     SGIVAAIAPKDLFEGVLFRGDPARAWLWVGIAFGLVFSLPYIVTLLTCRENPEFMALP
                     RKRFGSVKSFVTENFLVPFENRPFRYVMLMYLFGFMAQDAVLALAVYFLTYALGIPSM
                     MTLLVPVYGGLLVSLFLASKLAQRIGKRETFLVSGALWIGALILVLFMRPGMPVAVLY
                     AFGLAFGVGLAGVQSMVFAIFPDVPDVDELVSGARREGLYSGIFALFRKAGGAFILFL
                     VAALIGVAGYLPPIGDVKQAQTPAFVGSLMAIFVGLPALCVVLALFAAWRYPLTRERL
                     DRIRRINAALRRGETLSPGDEAVRAELLPLMVAGPRPGRGGHA"
     gene            complement(16853..>17582)
                     /locus_tag="P5313_07910"
     CDS             complement(16853..>17582)
                     /locus_tag="P5313_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRY80325.1"
                     /translation="LAWLLLATAAALPSAALETVTFPSADGLAVTADLYLAHGPDAPL
                     ILLFHQADYSRGEYREIAPRLNALGFNALAVDQRSGRSAQMVSNETAARARAAKKPQS
                     YLDALPDMRAAVAWVRSQPFGKGKLLLWGSSYSASLVLKMAGDEPGICAAVLAFSPGE
                     YFSPGDLIRTSAAKIRVPVFVTSGPFEKSDWEGIFQAIPPGSKVSFLPEGDGRHGSST
                     LWSGSAGNEAYWKAVESFLSGFRP"
BASE COUNT         2764 a         5981 c         5939 g         2898 t
ORIGIN      
        1 tccttgagtt tggccgagcc gggctccttg cccgagagga cccccgcggc ggtcacgaac
       61 accgcggtcg gcttccgggc ggcggccagg cgggccagga gacggcgggc ggggcccttc
      121 caccgccccg cggctacgga ggatcccacc acgaaggcgt cgtaggtctc gagctcccgg
      181 cggccgacct tccgggcgtc caggacgtgg gtcgggcggc cgtccgcctc cttcagggtc
      241 tccgcgatga gccgggcggt ccgctccgtc atcccccagc gggtcgaata ggcgatcagg
      301 gtcttcatgg tacctccgaa caaaaggccc ccggcgggcg ccgggggccg ttggaatcgg
      361 gcagcgcgac ggacagatcg cggaacctct tcgacgaacc ggggctccta cttgaaggtc
      421 cacttcttct ggcgggccag gtaggtccgg accaggtcgt agtccgagtc cttggcgggg
      481 gcgtagccct cgtggttgta gatcttccgc atgatctcca ggcccttggg atccttcacc
      541 atgttgatga aggccatccg gatggcctcc ttgagcttgg gatccaggtt ggggatcacg
      601 gagatcgtgt cgttggggat ctcgctggtg ttggagatca ggacgacctt cttcatgagg
      661 tccgggtaga aggcttcctt cgagaaaagg gtccgggcgt ccttgaaggt gaaggcggcg
      721 tccgcctggc cgttgaagac cgcggtgatg gcgttgtcgt ggccgcccac gttcatccag
      781 gtgacgtcct tctcgggatc caggccgttg tccgccagga ggttggcggg ccacacgaag
      841 ccggaggtgg aggtgaagga ggcgatggcg atcttcttgc ccttgaggtc cttgacgctc
      901 ttgatccccg agtccgctcc ggcgacaagc tgcgccttgt aaccggacac caggggcttg
      961 tccttgagca ggttgccgtt gtcgtccacg tcataccgcg tggatttcag gatggcctct
     1021 gcaaatccct cgtcggcggc catgacgtag gcggtggtgg caagaaggcc cacgtggatc
     1081 tgtccggcgg ccatgccctc gatcagggcg ttgtagtccg tggccacggt gacgttgacc
     1141 ttgcgcttga gctgggcttc cagcatctgc tgcatgggca tgcgctgggc gtccaggtag
     1201 gcggcgtccc gggaggggat cagctggacc tggatcacgt ccggattcac ggccttttcc
     1261 ttggtgccgt ccgcgaagag ggccagcggc agggccaggg ccaggatgag caagagggcg
     1321 atacggttct tcatgggatc ctcctataaa cgtgcggata ccgcgaacaa gattctcgag
     1381 atatggtact ctagcggggc cgttctgtac atacggattt tcttcggata cagtggcgga
     1441 tgccgcgcgg ggaccccgga ccgatccggt cccggccccg gatacgcccg ccgtccgaaa
     1501 ggagcgccca tgatccagtt caccaacgta tccaagacct accccaacgg ggtccaggcg
     1561 ctcaagggag tcaacctctc catagacaag ggcgaattcg tggccatcct gggcctctcg
     1621 ggggccggga agtccaccct cctcaggctg atcaaccagc tgatcgacac caccgagggg
     1681 gaagtgacgg tcaacggggt ggccgtgaac agggcgaccg gcaagaccct gaggaacgtc
     1741 cgccgccaga tcggtatgat cttccagggc ttcaacatcg tgaagcgcat gtccgtcctc
     1801 tccaacgtcc tgtccggccg ggtggcctac aacccgacct ggcggaccgt gctggggctc
     1861 ttcccggagg aggacaagca gatcgcctac cgggccttga agcgggtgga catcctggag
     1921 aaggtccaca cccgggccag cgagctttcc gggggccagc agcagcgcgt ggccatagcc
     1981 cgggccctgg cccaggagcc cctggtcatg ctggcggacg agcccgtggc ctccctggac
     2041 ccggttacca cgatccaggt catgaactac ctcaagaaga tcaaccggga ggacgggatc
     2101 accacggtgg ccaacctcca ccacgtggac ctggccctca agtacgccac ccgggtggtg
     2161 ggcatccggg cgggaacaat agtctacgac aagcagatga aggacatcga caaggacaac
     2221 ttcctccacg agatggagca ggtctacgga cgccggatcc actcggacga ggtcctgggg
     2281 gacgaggaat gagcggcccg gccgagcgga tcgtcgttcc caagccgaac cacctgcggc
     2341 ggaacctcat catcctgggg gtcacggccc tgatcgtggg gagcgcctgg ttcgcgggct
     2401 tcgacctggg ccggatcctc ctgaacctac ggaagggcgg ccgccttttg ggccgcatgt
     2461 tccttcggcc cgactgggcc tacacccccc ggatcgtgga tcccctcctg gagaccgtca
     2521 agatgtccgt ggtggggacc ttctggggcg ccatcctggc cttcccgacc gcggtcttcg
     2581 cggccaacaa cttcatcaag aacccctgga tcgcccagcc cctccgggtg atcctgaacg
     2641 tcacccggtc catccccgcc atggtcatgg cctcggtctt cgtggcggtc ttcgggatcg
     2701 gggccttcag cggggtcgtg gccctggtga tcttcacctt cggcctcatc tccaagctca
     2761 ccttcgagtc catcgaggcc atcgacccgg gccaggtgga ggccctccag gcggtggggg
     2821 ccaacaagcc caacgtcctg cgatacgcca tcgtgcccca gatcctgccc cagttcctct
     2881 cctacaccct gtacggcttc gagatcaacg tccgggccgc ggcggtcctg ggctacgtgg
     2941 gggccggcgg gatcggccag atcttcgagc agaacctggc ctggcggaac ttcgaccggg
     3001 tggggctcat catcatcatc tcgttcttcg tggtcctggt catagacctg gccagctccg
     3061 ccatgaggag gaagctcgta tgaccgaccg catcctggtc cggagggagc cgaaccccct
     3121 gcggaccctg tacaacatcc tgaactacgg cggactcgcg gccctggtcc tgtggagctc
     3181 ctccggcatc gagtacctgg ggatcatgca gaccgcccgg ggcaccgtgg aggccctgct
     3241 caagggattc gtcctcccgg acacggccta cctggccaac tggaccaagg acggcctgcc
     3301 ctacgccatc gtggagacca tcgcgatatc catcgcgggg acctttgcct cagccctcct
     3361 gtccatcccc gtggccctcc tggccagcca gaatatcgtg ggcccccggg tgtccaagct
     3421 gggcaaggtg atcgtgaccg cggtccgcac cttcccggag ctcatcctgg ccctgatctt
     3481 catcagcgtg gtggggcccg gggccccggc gggcatcctg gccatcggga tccactccgt
     3541 gggcatgttg gccaagctct tctccgaggc catcgagagc atggacatgg gcgtgaagga
     3601 ggccctggac tcctgcggag ccacccccat ggaggtcctc ttccgggcca tgctccccga
     3661 ggtggcgccg gagttcatct cctacaccct gtaccgcttc gagatcaacg tccgggcggc
     3721 ttccaccctg ggcctcgtcg gcgcgggagg gatcggagcg cccctgatct tcgccatccg
     3781 gaaccgggcc tggccccggg tggggatcat catgatcgtg atggtcgtcg tcgtgaccgt
     3841 gatcgacctg gtgtccggcc ggctccgcaa gaagctcgtc taggcccatg aacatactgc
     3901 tcgtcaacga cgacggcgtg gaggcccggg ggctggccct cctggcccgg gtgatgcggg
     3961 agcacggcga gctctacgtc gtggcccccc acagccagca gagcgccatc ggccacggca
     4021 tcaccatcca cgaccccatc cgggtccacg agcgggaggg tctcttcccg ggctccggcg
     4081 cccgggtctg gagcctggag gccaagcccg cggactgcgt caagttcgcc ctcttcgccc
     4141 tcgatctccc ggtggacctg gtagtctcgg gggtgaacga cgggccgaac atgggcacgg
     4201 atatcgtcta ttccggcacc ttggcggggg cctcggaggc ggtgatctgc ggcaagcccg
     4261 cgatcgccgt gtccacggac ttcgggtact tcgacctggc cgagcgggag ctggccgggg
     4321 tgatgtccac gatcctcgcc tcggggatcc tctccaagga caacgtcctc aacgtgaact
     4381 tcccccgccg gggcttcgag gcgagcctcg gcatgaaggt ctgcgacatg ggggaccggc
     4441 ccttcctcca cgagttcgtg gagatcgacg gggtctactg ggccaagggc acctgggggg
     4501 aggggaacaa ccccccgggc acggatgtct gggcctacaa gaacggttac gtgtccgtga
     4561 cccccatcca ggtgaaccgg accaactacg agtacatgaa ggtcctgcgg gaacggcttc
     4621 ccgcggccga gtaggccgga gggcggagga tggacggaat ctggcagcgc ctgggcataa
     4681 cgttcacgga accctaccgg gatcccctgt gggggaccat cctcttcccc tcggagttcc
     4741 tggacatcct ctccagcccc gagttcgtga agctctcccg gatcctacag ctgggcccca
     4801 cccacctggt ctaccccggg gccacccaca cccgccgggc ccactccatc ggggtcttct
     4861 ccatggccgc ccgggtgctg aaagccgtgg cccccggggc aggagacctg gtgacccccg
     4921 aaggcgcgcg ggccttcctg gccgccgccc tctgccacga cgtcggacac tttccctacg
     4981 cccactccct gaaggaactg cccctggagg agcacgaggc cctggcggcc cgggccctgt
     5041 ccgggcccat ggccgcggcc ctggagcggg ccggggcgga ccccgcccag gcggcgtcca
     5101 tcgtggacgt gaaatccccg gccgtcggga ccgggacggc gctgtaccgg tccctgctct
     5161 cgggggtcct ggacccggac aagctggact acctcaaccg ggacgcctgg gcctgcgggg
     5221 tcccctacgg ggtccaggat accgagtaca tgctccgcca catcgtcctg gacgaggagg
     5281 gccgccccgg ggtggacgac cggggggtca tggccgtgga gtccgtcctc ttctccaagt
     5341 accagatgta ccgggccgtg tactggcaca agggggtgcg cgccccgacc gccatggtca
     5401 agaaggcggt gatcgaggcc ctgcggaacg gctccgtgga ttccgccgac ctctacggcc
     5461 tggacgacgc cgggttctac gccctgatgg cggccccggg ccgggatccc gagggcctgg
     5521 tgcgctcggt cttcgacggc cggatcttcc cctcgtgcta cgaggccccc tatgacgagg
     5581 gcgtccccgc ccagcgcaag gccgcccacc tggtcacccg gaccgccgtg gagaaggagc
     5641 tggccgcggc cgtctccgcc cggaccggcc gaccctgccg cctggtggtc gacctcccgg
     5701 agcccctgtc cttcgagacc tccatgaacg tggccggtac cgggaagaag ttcagcgagt
     5761 ccgccacggt cttccgcccc gaggtggtcc ggggcttctc ccacagcctc cgggtcctcc
     5821 gggtcttctg cgacgcggac ccgggggccg cggcccccgc ggtggcggag atcctgacgg
     5881 ggcgcgccta gggctggatc ttcgggaaga gccccacgtg gcgcttgtcc aggatgacgt
     5941 tgtgggcctt caggcggatc gagttgagcc ggatgaagcc ccgggagtcg gtctggtcga
     6001 agccccccgc cacgtccatg ctccccagct cctggttgta cagggaggtg ggggaggtcc
     6061 gggccacggc catggcgttg cccttgtaca gggacatcgt gacagtcccg tcgatggcct
     6121 cctgggactg gttgaaggcg gccatgagga agtccatctc cggggcgaac cagaacccgt
     6181 tgtacaggta ctccgagaac ttgggctcca gcatgagctt gaggtgcatg acctccttgt
     6241 ccatggcgat cccctcgatg tcccggtggg cggcccagag gatggtggcc ccgggactct
     6301 cgtagatccc ccgggacttg atccccacga agcggttctc caccatgtcc acccggccga
     6361 tcccgttggc ccggcccaga tcgttcaggt actcgaagat cgccagggcc ccggtgaccg
     6421 attccttcgt gccctgccgg gagacccgga cgggcaggcc gtccttgaac tcgatgtcga
     6481 tccgagtctc cacgtccgga gcttccttgg gggagacggt ccgggagtag atgtcctcgg
     6541 cggccgggct ggcgggatcc tccaggatac ccgcctcgtg gctgatgtgc atgaggttct
     6601 cgtcctcgct gtagggcttc ttgaccgtgg cgctcaccgg gatgccgtga tcctcggcgt
     6661 acttgagcat gtccgagcgg ccctggaacg cggcaaggta ctcggagtcc ttccaggggg
     6721 agatgacctt gagctcgggt cccagggccg cgtaggtcaa ttcgaagcgg acctggtcgt
     6781 tgcccttgcc ggtggccccg tgggccacgt actccgcgcc ctcctcgtgg gctatgcgga
     6841 cctggtgctt ggcgatgacg ggccgggcca gggaggtgcc caggaggtag cggccctcgt
     6901 acagggcgtt cgccttgagg gcctggaaga tgtagcccgt cacgaactcc tcccgcaggt
     6961 cctccaggta gaccttggag gccccgatct tgagggcctt ctggcgtgcc ttctcgaaat
     7021 cctcctgctg gcccacgttg gccaggaagc agatgacctc gtagccttta tttaggagcc
     7081 actgcaggat gaccgacgtg tccaggccgc cggagtacgc cagaaccacc ttcgcgccca
     7141 tacggatacc tcccgatatt tgctgagggg atgcggaacc ggagcgccgc cgctgtcgaa
     7201 gccgatccgc ggagcgtccc atgtttctat cgaacgatac acagtagcat accgatccgg
     7261 ggccggagag tcaaaccccc ggcgggcgct ccttcgggcg ccccgcggcg actaggcggt
     7321 ttcgcagccc aagcggcgcg agatctcccg ggcgctgtcc ttgaccaggt cccggaccga
     7381 ctccatctgc cggtccgaga ggcggacgct ggggcccgag acggagaggg ccgcggcgat
     7441 gcggccgtgc aggtcgaaga tcggggccgc cgcgcagcgg accccgagct cgtgctcctc
     7501 gtcgtcgtag gcgaagccgg acatgcggat cctctggagc tccgccgcca gggccagggg
     7561 gtcggtgatg gtgtgctccg tgaacctctc cagcttggct tccttgaggt agcggcggat
     7621 gtactcgtcc tcctgccagg ccaggagaac cttcccgacc ccggtgcagt acaggggggt
     7681 cacgaagccg acacgggaga ccatggccac cttcagggaa aaggtggact cgatcttgtg
     7741 caggtagagg acctgatcgt tgtccaggat gcagaggtgg accgtctcgg aggtcatctt
     7801 cgccaagcgc ccgatcacgg gaagggccgc cttgttgagg tccagattcc ggatggcccc
     7861 ggacccgatc cggaagagct tgagggacag gcagtacttc tcggtctccc cgtcctgcag
     7921 gatgtacccg gcttccttaa gggtcgaaag gaggcggaag accgtgctct tctggcctcc
     7981 gatggcccgg gccaggtcgc tgaccccgca gtccccgtgc tcggccaggt actccagcac
     8041 ccggatcgcc ttttcgaccg cctgcacggt ctcgctgttt tccgccatgg atacccgcct
     8101 tcccttcctg cttcgattta accatgccct ggaaatgcgc gcaagaaatt tcgattcccg
     8161 gacgggtttg ttccgtttcc tgaccttttt ggaattgaca ggtttgagaa tcgggataaa
     8221 taatcatttt acggaacatc gttccaattt ccgttatcga gcatatccct gattccccga
     8281 ggtgcccatg agcgatcccg ctgcgttccg cgaacagatc cgcaggtccc ccgtagtcgc
     8341 cgtcctggtg atcgacgacg ccctggacgc cgtacccctg gcccgcgccc tggtgcgcgg
     8401 cggaatcctc gccatggagc tgaccctccg cacccccgcc gccttcgacg ccctgaggcg
     8461 gatacggtcc gaggtgccgg agctctacgc cggggtcgga accattctct ttcccgacca
     8521 ggtcgcccgg gtcaaggcgt cgggcgccgc ctacggggta tccccgggct tcaaccccgc
     8581 ggtggtggac gccgcccggg aggcgggcct gcccttcgcc ccgggagtcg ccaccccctc
     8641 ggagatcgaa gccgcctacg gaaagggctg cgacgtcgtc aaggtcttcc ccgcggaacc
     8701 cctgggaggg cttccctaca tcaaggccat cgcggcgccc tactcccacc tggggatcgc
     8761 ctacatccct ctggggggcg tgtccatgaa gaatctcgaa tcctacctgg agtggccgga
     8821 ggtcctggcc gtcggggggt cctggctggc cacccgggac ctgatcaagg ccaaggactg
     8881 ggacgcgatc agccgcaact gcgaggaggc cgcctccctg gcggcccgga tccggggcta
     8941 gcccatggcg aagttcgtca ccttcggcga gatcatgatg cgcctcaaga ccgaggactt
     9001 cctgcggttc cggcaggccc tcccgggccg cctggaggcg accttcgcgg gtgccgaggc
     9061 gaacgtggcc gcctccctgg ccctcctggg ggcggaggcc tccttcgtga cgaccctgcc
     9121 cgcccatccg atcgccgacg cctgcctggg atccctgcgg aacctggggg tcgacacggg
     9181 cggggtcctc cgttccggga agggccgcct gggcatctac ttcgtggagg ccggggccaa
     9241 ccagcggccc agcaacgtga tctacgaccg ggacggaagc tcggtggccc gggcggatcc
     9301 cgcctcctac gactgggacg cgatcttccg gggggcctcc tggttccact ggaccgggat
     9361 caccccggcc ctctcccggg aggcgacgga ggcggtccgc cgggccgttc accgcgccaa
     9421 ggacctgggg ctcacggtct cctgcgacct caacttccgc aagaagctct gggcctggga
     9481 ggcggggacc tccccgaacg ccctggcctc ccgggtgatg cgggagctcc tgcccttcgt
     9541 ggacatcctc gtggccaacg aggaggacgc caaggacgtc ctgggcatcg acgtcgggtc
     9601 cctggacccc tcccggggaa ccctggacgc gggccggtac ccggaggtgg cccgcgggat
     9661 cgtagagcag ttcccgaaca tccgcaaggt ggcggtgact ctccgggaga gcgtctcggc
     9721 cacccacaac gactggggcg gcatgctcta cgacgccgcc tcgggctccg cgtacttcgc
     9781 gcccctggag gacggagagt accggcccta cccgatccgc tccatagtgg accggatcgg
     9841 cgggggggac tccttctccg cggccctgat cctggccctg acggatcccg agctgggcgg
     9901 atccgacgcg gacgccgtcg ccttcgcgac cgcggcgtcc tgcctctgcc actcgatcca
     9961 cggggacttc aacttctcga cccgggagga ggtccgggcc ctgacgaagg gcaggacctc
    10021 cggccgcatc gtccgctgac ccggggacgg ccctttcaag gtgaaccgag ggacgagtcc
    10081 ctcaacaata ggaaatggag gatcgtatga aaagaacggc atggaatctc gcgggccggg
    10141 cgctggcggc ggtcgtgctg gccctcgtgg ccctggctcc ggtggcggcc caggccttcc
    10201 cccggaagcc cgtgacggtc atctgcccct gggctcccgg cggcggcacg gacaccatcc
    10261 tgcgcgccct gtgcaaggag gcggagaaga ccctcaagca gaccatcaac gtgacgaaca
    10321 acaccggggg cggaggggcg gtcggccact ccgcgatcat gcaggctccc gccgacggct
    10381 acaccgtggg catgatcacc ttcgagctca actccctgcc tccccagggg ctcatcccct
    10441 tcgactacaa ggcctacgat cccctcatgc ggatcaacgc cgacgccgcg gccctgacgg
    10501 tccacaagga cgccccctgg aacaccctta aggaattcat cgacttcgcg aaggccaatc
    10561 ccggccgcgt caccgtcggc aactccggtc cgggctcggt gtggcacatc gccgcgggtc
    10621 tcatggccca gaagaccggc accaacctcg tctacgtgcc cttcgacggg gcggccccgg
    10681 cggtcaccgc cctggtgggc aaccacatcc gggccgtgtc ggtctccgtg gcggaggtcc
    10741 ggggccaggt ccaggcgggt cagctgaaga tcctgggggt catggacacc cagcgcaacg
    10801 ccctcttccc cgaggtcccg accttccgtg agcagggcgt ggacgtggtg ttccacacct
    10861 ggcgcggact ggccctgccc aagggcgtcc cgcctgccgt caagaagacc ttgagcgaag
    10921 ccttcaaggc cgccttcgat tccccggact tcaaggcctt cgcggaaaag gccagcctgg
    10981 ccctggccta ccaggacgcc gacgagttcg gcaagttcct ggcccagaac gccgcggacg
    11041 tcgcggcggt gatgaagagc ctcgggctcg cgaagtaagg taggggaagg gccccgagag
    11101 gcaaggaggg tgaggatgag gatccacgac atcgcgggag gggcggtcgg ggccggaatc
    11161 ggcctgtacg ccctgatcca gggggcgaag atgcccccgg accacatcat gaagatcggg
    11221 ccgagtttct tcccgagcct cgtggcggcg gtgctcatcg ggttctccgc cctcctcgcc
    11281 ctccagggcg ccctttcccg caagcccggc ggcttcgaac gcgtggactt ccggtccttc
    11341 gggatctgga gggccctctt gtccctcggg gcggcggcgg cctacacggc cctgctgcgg
    11401 cccgtgggct tccttcccat gacggtcctg ttcatcctgg ccctcatgct gctcctggga
    11461 gcccgcgggc ccctcgggct cgtgctcgtg cccctggcgg ccacggcggg agtctggctc
    11521 gtgttcgagc aggtcctcct catcaccctc ccggtcggcc ttctgggcct gctgggattc
    11581 taggagatcc ccatggactt cgggctgttc ctgacgagcc tgggcaacgt cgtcctgcat
    11641 ccgagcgtcc tgctcatggt attcatcggc gtggccgggg ggatcaccat cggggccctg
    11701 ccgggcctga cggccaccat gggagtcgcc ctcctggttc ccttcacctt cgggcgcccc
    11761 cccaccgaga gcctggccat gctcctgggg atctactgcg gcgccatgta cgggggctcc
    11821 atctcggcca ttctgatccg cactccgggc accccggccg cggcggccac ggtcctggag
    11881 gggtatccca tgggccagaa aggccaggcg gggcgggccc tgtccatggc cctcttcgcg
    11941 tccttctgcg ggggcaccat cggggccctc atcatgacct tcctctcccc cgtcgtgtcc
    12001 cggttcgccc tggagttcgg cccggtggag tatttcgccc tggccctctt cggcctctcc
    12061 gtcatcatct ccatctccgg cgacagtgtc gtgaagggag tcatgtcggg gatgttcggt
    12121 cttctcctgg ccaccatcgg cttcgatccc atctccggct tcccccgcta caccttcggc
    12181 cgggtggaac tgttggaggg acccgccttc atccccaccc tgatcggcct gttcgcggtc
    12241 tccgagatcc tgaactccat cgagaagatg ggcgtcctca aggagatcaa ggcctcgatc
    12301 gaccgctaca tcccgtcctg gcaggacatc aaggtttccc tgaagaacat cgtgaagtcc
    12361 agcctgatcg gcacggcgat cggatccatc ccgggagcgg ggggggacat cgcggccttc
    12421 gtgtcctacg gcgaggcgaa gcggtcctcc aagcatcccc acctctacgg aaccggggtg
    12481 atcgagggcg tcgcggctac ggaggccgcg aacaacgcct gctccggcgg ggcgatgatc
    12541 cccctcttgt ccctgggggt gcccggggac gcggtgaccg ctgtgctcct cggggccttc
    12601 gtcatccagg gcctccaacc cggccccctg ctctaccggg agcacctgga cgtggtctac
    12661 aacgtcttcg cgtccatgct cgtggccaac gtggccatgc tccttgtcgg gatggcgggg
    12721 atccgcttct tcgcccggat catcctggtc aagcgcgaaa tcctcaaccc cgtcatcttc
    12781 gtcctgtccc tggtcggttc cttcgcgatg cggcaaaaca tcttcgacgt ggggctcacc
    12841 ctggccttcg gggtgctggg ctggatcatg atgcgggcca agttccccct gtcccccatc
    12901 ctcctggccc tgatcctggg ccccatgacg gaagccaacc tccggcgggc cctgacgatc
    12961 tcccagggcg actactccat actggtctcc cggcccatcg ccatcgccct cttcgtgctg
    13021 gcggcgctct ccctggtcgt gggcatcctg aaccagcgaa agatccggaa gctcctggcg
    13081 gagtcggtcg agaaggggca ggagcccggt tgaaccttcc gggcgtcgga taccgggtta
    13141 tggaaaattg ccatcgcccg gtatccggct cctgactccc gtcaccggac ttccccccac
    13201 gcctcggccc ggcgccggat ctcctccggc ggctcggggc cccggaaggg gaagacggcg
    13261 aaggaaaagc ggctggtctt tccgttatag gagggtgcgt agctgtccaa ctggtccgcc
    13321 atgagcaccg ccatcgcccg gcccaggccc gtgaccgcgg tggcgtactt ccactgggga
    13381 ccgtagtagg tcccgaaggg gttcagccgg agacgctggc ggccgcccga gacctcggac
    13441 cgcatgggac agaaagcgaa attgcacctg tccccctcgt actgggccac caggaggccc
    13501 tcggagccgt tcgaaaccgc gacccagggg tgggcgatgt ggttgttgaa ggaggcggtc
    13561 cggcgggatc cggagaccag gtggtggtcg accgggtagg cgctcacgtc cccgaagaag
    13621 ttgcgcttga tcacccggaa gggccgggcc gggtccgcgt cgaagagagg aaggatctcc
    13681 gcgggcagga cctcggccca ccggtggtcc caggtccggt ccagcctgag ggccttggcc
    13741 ttgtcgtacc cgcggtgcgg ggtctcgggg tactcgagct ccagggacag gaacaccgcg
    13801 tcgtccccgg caaggcgggt ctcgatccgt gtccaccggc cgtcccggat ccggccgggc
    13861 agggacaggg ctccccggag ggtccggacg gccgtcccgt ccggccgagt ttcgtccccg
    13921 acttcctcca cggttccggc acggacgcgg ccgccccagg tgatccgggg gtccaggacc
    13981 cggaagcggc cgccgtcccc ggctctcccc tccgcccggc cgcttcggcc ggaggcgggt
    14041 gccgaatcgg gcgcgggggc gggcggcggg aaccgcgatt ccggggccgc cttcgccccg
    14101 gccgagacgc cccgccgctc ggccaggacc gccgcggcca gctccccggc ccggcgcgcc
    14161 cgggcgaatc cgtcttccag gcggcccgcg tgcatcaccg gactggccat gccgaagtgg
    14221 gtcgtggaca gggccaggag gcgctcccgg agggactcgg ccaggcgcgg gtcgtccgtg
    14281 ggggcgcccg cggctacggc cgcgtcgtac aggcgccggg tgcgctggat caccgtccag
    14341 agctcggtgt tctcccattt ctccgcccag gaggaaagcc cgtcgaagct cccgtcggcg
    14401 gtgtcctggt ccaggaccac ccggccgacg gggggatggt cccggaggta gtccccgggc
    14461 cgggtgaagc ggaggaaggg caggtccgcg acggagcgga ccatccgggc caggccgtcg
    14521 aaggacggca ggagccgtga caccacgggg atcttcatgc ccgcccagaa ctcgtcgtcc
    14581 gcgtcggcgt ccaggatcag caagaggtcc cggggctcct ccatccgcag ctggcggcgc
    14641 cggaggccct tcagccagag ccggagggag gccccgcggt ccgccacgtc cccgtggttg
    14701 tagcagggca ggagggtcat ggagcccgcc aggctcgagc tctccagggt cagggggttg
    14761 taccgctccg ggaaggccag gggccggacg aagtttgaga aaccgttgaa gggaaccgcc
    14821 gagtagtaga ggctgatcgc cgggattccc gccgcggggt accgggacag gaaagagggg
    14881 gtgtacatgc actcctgggg ccggaggatc gggacccagc ggccgaagac gtcggccagc
    14941 cccgaccggc ccgggttgct caccgccagg cgggccagct cctcgaactc ctcggccgtg
    15001 caggcgctca cgatcccgtt gttccaggac atgggctcga tctcgtcccg atcctcccgg
    15061 accctccggg cgatgccctc gaccaggtcc ggagcgtggc gcggcatcag ggtttccagg
    15121 ctgtagtagt tctcgatatc ccaggtcccc cggacgggta tgccctcgga gttcaggcgg
    15181 tccaggtcgg ccaggatccc ccggatgatc cggatgtcct tgccgattcc ccgttcgtcc
    15241 agggagtccc cccgatacga gtggtagaag ttgacgtgga agcggaaggc caggtggacc
    15301 gcgggtccgg actcaggcat ggccgccccg tcccggtcgc ggcccggcga ccatgagggg
    15361 caggagctcc gcccggaccg cctcgtcccc ggggctcagg gtctcccccc gtcgcagggc
    15421 ggcgttgatg cgccggatgc ggtccaggcg ttcccgggtc agcgggtagc gccaagccgc
    15481 gaaaagggcc aggaccacgc agagggcggg caggcccacg aagatcgcca tgaggcttcc
    15541 cacgaaggcg ggggtctggg cctgcttgac atcgccgatc ggcggcaggt atcccgcgac
    15601 accgatcagc gccgccacca ggaagaggat gaaggccccg cccgccttgc ggaagagggc
    15661 gaagatcccg ctgtagaggc cttccctccg ggcaccggag acgagttcgt ccacgtccgg
    15721 tacgtccggg aagatggcga acaccatgga ctggaccccc gccaggccga ccccgaaggc
    15781 caggccgaag gcgtacagga ccgccacggg catgcccggc cgcatgaaca ggaccaggat
    15841 cagggccccg atccacaggg ccccggagac caggaaggtc tcccgcttgc cgatcctctg
    15901 ggccagcttg gacgccagga aaagggaaac caggagcccc ccgtatacgg ggaccaggag
    15961 ggtcatcatg gaggggatgc ccagggcata ggtcaggaag tagaccgcca aagccaggac
    16021 cgcgtcctgg gccatgaagc cgaagaggta catcagcatc acgtaccgga agggccggtt
    16081 ctcgaagggc acgaggaaat tctccgtgac gaaggacttc acggaaccga agcgcttccg
    16141 gggcagggcc atgaactcgg ggttttcccg gcacgtgagg agggtgacga tgtagggaag
    16201 cgagaacacg aggccgaagg cgatgcccac ccagagccag gcacgggcgg ggtccccccg
    16261 gaagaggacc ccctcgaaga ggtccttcgg ggcgatcgcc gccacgatcc ccgagaggtt
    16321 ggagaagacc atgcggtaga aggacaggcg ggtccgctcg tcgtagtcct cggtcaattc
    16381 cgcggcgatc gagttgtagg ggacccagac catggtgtag accgtggaga agaggacgta
    16441 ggccgtcagg tagtaggcga aggccgcccc gggacccgcg aagcccggcc ggacccacat
    16501 cgcggcgaag gagaggaaga ccagggggat gcccgcgagg aagtagggcc ggcgccggcc
    16561 gaggggcgtc cgggtgttgt cgctgaggag gcccatcacc gggtccgtga ccgcgtccca
    16621 gagcttgctg accaggatcg cggttcccgc ccagatcgga gacagtcccg ccatgtcggt
    16681 caggaagtag aggtagtaca tgttgaggat ggtcgtgctg ccgccgccgt agatgtctcc
    16741 catgccgtag ccgatacgga cggacaagcc gaggggcttt cgttcctggt tcatgattac
    16801 tcctccttcc cagcggttcc ggccggggca ccgcaaccgg gttgacggaa tctcagggcc
    16861 ggaagcccga aaggaaggac tcgacggcct tccagtacgc ctcgttcccg gcgctcccgc
    16921 tccagagggt cgaggatccg tggcgtccgt cgccctccgg caggaaggac accttcgaac
    16981 cggggggaat cgcctggaag atcccctccc agtcgctctt ttcgaagggg cccgaggtca
    17041 cgaagaccgg gacccggatc ttcgcggcgg aagtccggat caggtcgccc ggggagaagt
    17101 attcgcccgg ggagaaggcc aggaccgccg cgcagatccc cggttcgtcc cccgccattt
    17161 ttaggaccag ggaggcggaa taggagctcc cccagagcag gagcttcccc ttcccgaagg
    17221 gctgggaccg gacccaggcg accgcggccc gcatgtccgg cagggcgtcc aggtaggact
    17281 gcggcttctt tgcggcccgg gcccgggccg cggtttcgtt gctcaccatc tgcgcagagc
    17341 gccccgagcg ctggtccacg gccagggcgt tgaatcccag ggcgttcagt ctcggcgcga
    17401 tctcccggta ttccccccgg ctgtaatccg cctggtgaaa gaggaggatg aggggcgcgt
    17461 cgggcccgtg ggccaggtag aggtccgccg tgacggccag gccgtccgcg gatgggaagg
    17521 tcacggtttc gagggcggcg gatggaaggg cggccgccgt cgcgagcagg agccaggcca
    17581 ac
//
LOCUS       DAOTEI010000063        17551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2180_length_17551_cov_6.261031, whole genome shotgun sequence.
ACCESSION   DAOTEI010000063 DAOTEI010000000
VERSION     DAOTEI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 17551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17551
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2180_length_17551_cov_6.261031"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="P5313_07915"
     CDS             complement(<1..261)
                     /locus_tag="P5313_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY80326.1"
                     /translation="MVETRFRVLAVDDDFTLLERIKAITGRIDYPRIELHTAETVRGA
                     LEVLDSRSIDLVLSDFRLPDGNGLDVLRKVKSSDPTVSVVIMT"
     gene            599..10708
                     /locus_tag="P5313_07920"
     CDS             599..10708
                     /locus_tag="P5313_07920"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY80327.1"
                     /translation="MRLNTGRFPIGLHIFTILLFLFGGCRNPFSPGLGEKIDITPPSI
                     SLTSHLPGGFLQGEVSFSGLASDDQKIAAVRISFNGGTSWSDVTSYDTASKVWRHDLD
                     TTSYPNGSFRIRLRVLDEAGKRTDTEDLVFTIDNLPPQIEIQVPAINPATYDPLLPPE
                     IAMNGVVIGIVTDLQGVAVGYPQIKFWKTTDSEPDWDDVIGLSEVKKTTDFRYRLSDE
                     HPGEVGTFNMRLRAVDVSGGETVVPPLAQDPYTVKVISGDVPPVINLAFTSGDINVVA
                     GGTYAGDDYAFRVTANHPNGITSVTLERTREGGPTVPVSWAADDLGSPTRTLDSPVLA
                     VAPDGSDDGTYEFRIEAVSSTGSVANYYKTVIVDATPPSVEITNLSPTVVDDTDPGNP
                     VEYINGVIQINALATDANAVLGSKWWMLPSVDPAPADYDSPGGTAFATSTPSLTLDST
                     SLLDGTEYKFYIISRDRARNDTRISRVLTVSQGTDIPTAVVTDIDTSVTTAAGAGAGG
                     VNLLDTNARIRGILSDDDRIDVSSVTLSLNGAPVGTLDSLGLPGRSVSFSHNVDSLGQ
                     GVHSFELEFGDDASVKSGKPSASASIGPVYFVIDTSPPSISVVSPGVGTFQRASFTLS
                     GTAADANGLATVTPGGSDEYIEVAFPEAPGTWVQVPVDSGTHAWSVTVGGGPSTLFGG
                     SYEGPRSVSIRARDRFGKLSTASFSFTVDTVAPTAAVSLPAADAWLSGASDSVQGTAS
                     DATSSVSQVRYWVGDEGASPPADTADWTLASGTTSWNGSLNLSALGEGRTSVHARSRD
                     AAGNWSVSSVRTFGIDQAAPAITETAVGAASAGRNAAFSLGGNLSDSNALANLTVTQT
                     KGADPAVELYSGSVTGTSQAWSISDLPRNPAIPANLLLEDGVYTYALTVTDAAGKTAV
                     LGRTITVDLTGPDVAIVAPTPGAAVQGTALSANGTAADFGGVSPIAETRYAVTVPPAL
                     PPADYAAWTAASGAASWTAALSFLPSEEGPRVLHVVARDTAGNWSPAPATAAFNLDQA
                     PPEVQAFGFGVGTVNTVGGTGYAKGDFSFSFTAYDSNALANVRVTRNGTQIFDGNYST
                     TSQPVTVNQTVGGPGLADGIYEYILTVTDIVGKTATLTRSVVVDTAGPLAEIASLTPV
                     LSMSGTDYVNGTIRFTASASDGNGLTGVKWWLLPVAASAPADYDAAGGTAFGAAPYTA
                     TVDTTSLSDSTAHVLYVIARDRALNDTQTTRTVTVEQSTDIPAVSLTHPGAGAFVGAG
                     YTVRGTVTDDDGVAAATVQVSYHDGSSWSAWGSATVTGTGRELSFTYVLPVGIGADGP
                     KQVRARADDDAAAKLGGDPSASGTSSDVSFTLDTLPPSVSILVPAAASARRTTFTVSG
                     EVVERNLDTLRLSLDGGPQVDAPVVGVGETKTWSYDLPGADFDALAEGPHTISVEAVD
                     RVGRSHTVQRVFYKDTSGPAISFTSIVEGGNTIVTETSPVLRGAFSDEYSDIAGTFEY
                     RLDSSLDTDPWTTLAVTGTGKTVAWAVSVDALSDGSHTVDVRILDAVGNLRTKEDVAF
                     RLDRALPVINISAPVSGGIYGAVAAGPVFTLSGTAQDANLVSVRAKLGSGPETDITGV
                     VAGGTSTWNYPVAKADFDALPDGGGTVTVTAVDAAGRTSQTAWTFTKDTTEPDAAFNT
                     LDLGGTTVLQDSQPRVLGSFSDAYGVASMESRVERYDYAGTLWILVQDWTSLGAPGGQ
                     TVAAWSKDLGTAGLNLADGRYRIAVRASDRAVPDANVRTTAAVEFRIDRNNPSLTLNP
                     GGLFQRTDFPISGTASDLNTIASVRAKVGDGDFSSGTVTSASTSNGFATWTVTVPTGG
                     LTPGSYTVYVEAEDGAGRKSQLTREFSFDGTPPVVSISEPPNGSRVNGLVTVRGTTSD
                     DNAVALVQYRIGNGATTWQTAGLGGGLYSWSYTFSNINTYANTSDVTEVDPLTGLPAS
                     GTNVWALPFQVRVTDVAGNTTEELSYRLLVDPDMDAPVVTIISPMNDQIVGGEVRVSG
                     YASDDDWVRSVELRVDPTGTGTSFGAWEPATMVNQGTQVNWFKNINTSGELNPAAGTI
                     RDVRIQVRAYDSKDYGVTPGITGDTVELNVKFDSGVPVIQNVRVFRNGSEVEYAAGVR
                     AAGIFSVRASVRDEGGIASIKWRREGQTVFTNILADPAYTTPPTERTAGFFEVGRKYL
                     ITSAGTTDFTAIGASSNTPGISFLATGMGAGTGTAYEAIGPLADPTNQYFQYEVEIEI
                     DSTTVNAGQYAGTTGFFSLDLQATDNATPTPYLTQASLNIQIDNYFPFGEYSSSARAS
                     TANYYIQGRAWDSGTGSGSIQGIDRVVVYFYKNGVYIAPDGGAFNGTTRTLKDMTAGG
                     TLQTVVFPADAASGIVIDGNEVTSDLDGSGYIEGFTDDGIYKNWYAIYDTTQLGDGPM
                     DVHYVVVDRAGNSTRYVQAVYIRNNAPRITGVVIGTDVDGNGTVGDIASGESRRIAAA
                     YGTTNFTVRNERLAFEAETAGGNLPNRYSIGYVTGTASVGAAALVPGSVYTIETAGTT
                     NWTLLGAPDNLAGTTFVAGGSGAGTGTATGYTIVQKNTAPYTSATLTLDDFTGMPDTA
                     AANEARFVVKVYDTTVAAGTEADQLADAVVIGLSIDNVDDLAPGITAAPFGREYTSPA
                     SFSDADKALKDVDAYTENLVTSGSGVSLVKHGYAQYALHSGDGDADVSGKVVFLGKAA
                     DNQRIARITVQIPGYNGGAAFDIALWDGSALSAAAAGRTLADVAAGTSDWGFEAEAGS
                     QAVTETYGHVLNWKFAWNSAALSTVTAPDVIVTFTAYDAGPSGGNSSSGDLTVDVVPY
                     ITKISNPTGQGGLSDTVIRSSRGRYSIEYHATNHFRASGFNLSGAVAHVSSAAIDIQP
                     GTTNLTVAATTATTVDIRKNFSLSGYLTVFVNGIPSINNLNDTGQAYHSEAAVGDPRS
                     TLWTDDRYLTVWNLTQVLPAVTNQTFYYPSMVMNGNQPLFSYANDNDGYTYRTTGVAA
                     SSQRGGIWFERHTALARSAGGIYWILSVQDAVGTGGIGYLYLNRDRNVNALLGDTQNN
                     NHFEIIGLDYTSRQMNRIRYPKLIVEGPDNATNIYISYYDAHPSRRELSFVSFQASTA
                     TASNLTQPANDAARAAQVQVIPNTANASSEYFDMVRIGAAEVAVAYFDETAGVLKLQY
                     SGDAWNANNQSGAGTWTELTVDDSVLTGSHVSMTVGQRSGTTYLYLAYHDAAETALKF
                     AQIDWTTKAVTKSVIDNRFSVGTRTNVFVIDELPYVSFYSDSYSGTRNSIRLAYPVAA
                     NGRTAAENILQPGADVNEEYTGDWEVAAVPAVTVPKGGIEQFNRTQLNQYTNGASVLP
                     VVGWLADRIEYAELQPTAP"
     gene            10722..11777
                     /locus_tag="P5313_07925"
     CDS             10722..11777
                     /locus_tag="P5313_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80328.1"
                     /translation="MKPSVRRTDKRAGNHRPGIPGPGRFKIIAAAAALAAVLAAGYLL
                     LRPRPVFLVEEGFVPAWEKLLEEGPGAPGWRIVKYDPRDGLPSTRGLRGYRISTDREG
                     RPPAGRSAAAGSAESVPIPVPRRDLSRNPALPDGALPLAVDPWMVFRRHADPPPSRAL
                     LEKGGSGAVVLLPGGEPEALWAWAGQELQNVPGSFPSDPGLWLDRTDRMARDRRFQPG
                     ALTARWEDVLSRLLEPGASWLYAPLSRVLTLPGDRTSLLEADPFPPREGWNDFGLQTR
                     ILWAVPLGRGTARGFMASAARWLGDPAIQTRLGAILDWAPAHRDARAASPLARTSQEA
                     WLKSTYVWEAPEYAHID"
     gene            11830..15834
                     /locus_tag="P5313_07930"
     CDS             11830..15834
                     /locus_tag="P5313_07930"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY80329.1"
                     /translation="MILASSVPAWANGTPEETWQEATGTEVWLREFDLSGAKPGTHNV
                     VIRARDSAGNEALVGPYNIRIDPSVGLPTVRIIYPEKGTVLRRDVHIVGVAGGRIGIQ
                     AVEIKLDGGEYSPASGSEYWSASVDAASLPDGRHTLTARAVDPRGTSGPETSVSFILD
                     RAPPSVRIRSHESGALIRGAVQFEGTADDANGIARLSLSVDGGKTYQDLGFRGARGET
                     EVGFSFPVQSGKLPDGAAIYYLRAEDGTGAVSVNPVLFFVDNKSPELEILWPEKGATV
                     AGTFRITGTMKDAVGVQSLSCEVGKSVLEIEMRPGDPYWTADIPVPPGTGRSVDLRIV
                     AIDRSGNRTSVSRSFVNDLGSSIPSVKISHPGEAGLATFPADGAIFGYVLGGAGVDAV
                     KVEGGKTYPARPGFRIPSSDIGPGRSELRLQASGPEAAPGEVLKVRVARPALPSGTLP
                     PSAPPPSNITVTSPAPYSYQRGDFSIAGTAEAPAGSRLEYRTAPTESWQEISLGGDGR
                     FSSRVAPAALPEGPMHLELRTVTDGTAAEPVYHPVNRHTTAPEIRIHSPRSGEKVNGS
                     VTVSGDVVTAAPIADLSYTLDGKTFRALQGIPASYGAAFSVIVDFTVLQKSGGSVGVR
                     AVDAAGNSADIGIPVTVDASGDLPSVQLNLPVDGAVATGDFDISGMAFDDDGVAAVHW
                     RIGEGAFSRLDVKQSFRIGVPLSSVRDGERRIEVYAEDVYGRVGPPASAVVRISTGAP
                     TVQVVSPSIEAYNRGTVILRGTARDPNGIFGVRVSMDNGATFQNAEGTESWSLSLNTG
                     AYKDGVYSVLVVAADSFGVESRISSLLNIDNTPPLISLGEPADGRVTEPKLRVSGQTS
                     DNSGVESLAFEISPVSGSGAQRGLRIEAEPRLVILETLDVSSLPGGKYNLRMIASDAA
                     GNTSVLVRGIELVKEAGAHRVVLLDPLPGVAHTGPLTVSGRVTGPSLPASVGIFTGQT
                     RAADAEVDRFGYFRHTLEGIPEEGRLVLWAGFDTSAGEKVRSADHAVILEPYGPVLTI
                     LSHGAGDAVTGRPWLSGTAGIRLSPGEAEAAGKKAARYYEVSNVAISLDNGRSFQPAQ
                     GTSEWKFRLETAELPRGPLPVLVRAEYADGRAVVRRILLTVDTVPPVVTLVAPQEGSL
                     HAESLLMFGTASDDYGLETLEVNLRAGDKAGYALPQFIQGLYLDTHALGATYGDVGLG
                     VSFFDNNVKLQLQAGMAPPGRFTGTVFGAKLLANVLYLPFDYFLGPDWSFFSMSLALG
                     ANFSYFTMDAGSDPLVMSSVLVQWEFARFQFPEWKAFRTFSLYAEPNLWFAASDVEAG
                     AIFKLAFGFRIGIF"
     gene            15938..16648
                     /locus_tag="P5313_07935"
     CDS             15938..16648
                     /locus_tag="P5313_07935"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HRY80330.1"
                     /translation="MLLTPSFLSAARDYRLLLDRGYPTAASLKLVGDRWALDKAARTV
                     LFRGILPESLSAAIAVRLVRVLPPGARLAVDGYNVLFTLSNYRRGHPLFLGTDGLVRD
                     AGGAHGRIQDQGLFLECARAATERLADLGVAYAEVYLDAPVSGSGSHAAVLRDLLARA
                     GVTGRVELAASADGPVRDFEGGAAGYAAGQAEGFVATSDSAVAVAARSPVFDLARDIL
                     EARYRAVFPDLGVVVRSS"
     gene            16781..>17551
                     /locus_tag="P5313_07940"
     CDS             16781..>17551
                     /locus_tag="P5313_07940"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HRY80331.1"
                     /translation="MQSEAGTLHKTVLEEIERVRPDAVRISRSIHGHPELGLQERWAS
                     AFLAEEARKRGFEVEAPVAGLSTAFAARYTSPKPGPKVGFLCEYDALPGLGHACGHNI
                     IAAASCAAAFALKTLAEKLGGSVFLFGTPDEEAMSEESKGGKVIMSRAGLFRDLDAVF
                     MTHPAGGSDAAWRYNFPLKDFTIRFKGRPAHYTQPEKGINALEALLLCIANLNALKRA
                     WSPSVLLAYTIIDGGAPSAIIVPETAKAKTREAKATLEQ"
BASE COUNT         2987 a         6009 c         5706 g         2849 t
ORIGIN      
        1 ggtcatgatg accaccgaga cggtcgggtc ggagctcttg accttccgga gcacgtccag
       61 gccgttcccg tcgggcaggc ggaagtcgga caggacgagg tcgatcgagc gggagtcgag
      121 gacttcgagg gcacctctca ccgtctccgc ggtgtgaagt tcgatgcggg gataatcgat
      181 ccggccggtg atggccttga tccgctccag cagggtgaag tcgtcgtcca ccgcaaggac
      241 gcggaaccgg gtttctacca tgcgtataag tatcggctcg tccgccccgc aaagccaggg
      301 tgcttcgacg ttcaggtcga aaacttcgac ggaatcgtcg aagcccggcc cgtccctaga
      361 agacctccgg ggcggccggg tatcgggcta tccgggtctt ttcatgtatt tcattttata
      421 gaaaaacttt aaagcccggt ggacggattt tgctcttttg gcatgaaacc tgctttaagt
      481 catctgaaca ggatgcgata cccggccgaa acagcctccc gccgcatgcc cggggcggcg
      541 gtatttgtaa cggctatata ggaaagcgtc cgataatgat tccgggtaga ggtgcacaat
      601 gaggttgaat acaggccggt ttcccatcgg acttcatatc ttcacgattc ttctattcct
      661 gttcgggggc tgtcggaatc ccttctcccc gggcctgggg gagaagatcg atatcacccc
      721 tccgtcgatc agtctcacaa gccatctgcc gggaggcttc cttcagggcg aggtatcctt
      781 ctccggcctt gcctcggacg accagaagat cgcggcggtt cgcatatcat tcaacggggg
      841 gacctcctgg agcgacgtga cctcctacga caccgcgtcc aaggtatggc gtcatgacct
      901 ggataccacg tcgtacccca acggatcctt caggatccgg cttcgcgtgc tcgacgaggc
      961 ggggaagcgc acggacacgg aggatctggt cttcaccatc gacaacctcc cgccccagat
     1021 agagatccag gtgcccgcca tcaatcccgc gacgtacgat cctctcctgc cgccggaaat
     1081 cgccatgaac ggggtcgtca tcggtatcgt gacggacctc cagggtgtcg ccgtcgggta
     1141 cccgcagatc aagttctgga aaaccaccga ttccgagccg gactgggatg acgtcatcgg
     1201 cctatccgag gtcaagaaaa cgaccgactt ccggtaccga ctctccgacg agcatcccgg
     1261 cgaggtcggg accttcaaca tgaggctccg ggccgtggac gtgagcggcg gtgagaccgt
     1321 cgtcccgccc ctcgcccagg atccctatac agtgaaagtc atcagcgggg acgtacctcc
     1381 cgtgatcaac ctggccttca cctccgggga catcaacgtc gtcgccgggg gtacctacgc
     1441 cggggatgat tacgccttcc gcgtaaccgc gaatcacccc aacggaatca cctctgtgac
     1501 ccttgagaga acccgggagg gtggcccgac cgttcccgta tcctgggcgg ccgacgatct
     1561 gggatccccg acccgcaccc tggacagccc ggtccttgcc gtggcgcctg acggctccga
     1621 cgacgggacc tacgagttca ggatcgaggc ggtcagctcc accggaagcg tcgcaaatta
     1681 ctacaagacc gtcatcgtgg acgccacacc tcccagcgtg gagatcacga acctttcgcc
     1741 cacggtggtg gacgataccg atcccggcaa cccggtggaa tacattaacg gagtgatcca
     1801 gatcaacgcc ctggcgacgg acgccaacgc cgtgctgggc agcaagtggt ggatgctccc
     1861 ctccgtcgat cccgccccgg cggactacga cagtcccggg ggaaccgcgt tcgccacctc
     1921 gaccccgtcc ctaaccctgg attccacgtc cctgctggat ggcaccgagt acaaattcta
     1981 tataatctcc cgggatcggg cccggaacga cacccgcatc agccgggtct tgacggtcag
     2041 ccaagggacg gacattccga cggcggttgt aacggacatc gacacctccg tcaccacggc
     2101 ggccggagcc ggcgcggggg gagtgaacct cctggacacc aacgcccgaa tccgaggcat
     2161 cctgtcggac gacgaccgga tcgacgtttc ctccgtgact ctcagcctga acggtgcccc
     2221 cgtggggacc ctcgactccc tggggctccc ggggcgcagc gtctccttct cccacaacgt
     2281 ggattccctg gggcaggggg tccactcctt cgagctcgag ttcggcgacg acgcctccgt
     2341 caagagcggg aagccctcgg cctcggcctc gatcggtccc gtgtacttcg tgatcgacac
     2401 ctctccgccc tcgatctccg tcgtttcgcc cggggttgga accttccaga gggcgtcctt
     2461 caccctctcg ggaaccgcgg cggacgcgaa cggcctggcc acggtgacgc cgggaggatc
     2521 ggacgagtac atcgaggtag ccttcccgga ggctccgggg acttgggtcc aggtgccggt
     2581 cgattccgga acccacgcgt ggtccgtgac cgtagggggc gggccgtcga ctctcttcgg
     2641 cggttcctac gaaggtcccc gatcagtctc catccgcgcc cgggaccggt tcgggaagct
     2701 ctccacggct tcattctcct tcacggtaga caccgtagcc ccgaccgcgg ccgtctccct
     2761 gcccgcggcg gacgcgtggc tctcgggagc ctcggattcc gtccagggga ccgcctcgga
     2821 cgcgacgagt tccgtatccc aggtccggta ctgggtcggc gacgagggag cctcaccgcc
     2881 ggcggatact gcggactgga ccctagcctc gggaacgacc agctggaacg gctctctcaa
     2941 cctttcggcc ctgggggagg ggaggacatc ggtccatgcc cggtcccggg acgcggccgg
     3001 caactggagc gtatcctcgg tccggacctt cgggatcgac caggccgccc ctgccatcac
     3061 ggaaacggcc gtaggcgcgg ctagcgcggg acggaacgcg gccttctccc tgggcggcaa
     3121 cctttcggac tccaacgccc tcgcgaacct gaccgttacc caaaccaagg gcgcggatcc
     3181 cgccgtggag ctgtacagcg gatccgtgac gggaacctcc caagcctggt cgatctccga
     3241 ccttccccgg aaccccgcga tccccgcgaa cctgctcctg gaggacgggg tctacaccta
     3301 tgccctcacc gttacagatg ccgcgggaaa gaccgcggtc ctcgggcgga cgatcaccgt
     3361 ggacctcacc ggacccgacg tggccatcgt cgccccgacg cctggagccg ccgtccaggg
     3421 caccgcccta tccgcgaacg gcaccgccgc ggatttcggc ggggtgagcc ccatagcaga
     3481 aacccggtac gcggtgaccg tgcctccggc ccttccgccc gcggattacg cggcatggac
     3541 cgccgcttcg ggcgcggcgt cctggacagc ggctctgagc ttcctgccct ccgaggaagg
     3601 tccccgggtg ctccacgtcg tggcccggga taccgcgggc aactggagcc ccgccccggc
     3661 cacggcggcc ttcaacctgg atcaggcgcc gccggaggtt caagccttcg gtttcggtgt
     3721 gggaaccgtc aacaccgtcg gtggcaccgg gtacgccaag ggggatttct ccttctcctt
     3781 cacggcctac gacagcaatg ccctggccaa cgtccgggta acacggaacg ggacccagat
     3841 cttcgacgga aactactcga ctacaagcca gcccgtcacc gttaaccaaa cggtgggcgg
     3901 ccccggactc gccgacggca tctacgagta catcctcacg gtgacggaca tcgtcggcaa
     3961 gaccgcgacc ctgacccgca gcgtcgtcgt ggataccgcg gggcccctgg cggagatcgc
     4021 cagtctaacc ccggtgctgt cgatgagcgg caccgattac gtgaacggga ccatccgctt
     4081 caccgcctcc gcctcggacg ggaacggcct gaccggggtc aagtggtggc ttctgccggt
     4141 cgcggcttcg gccccggcgg attacgacgc cgccggtggc accgccttcg gggcggctcc
     4201 gtacacggcc acggtggata cgacgagcct gtccgattcg acggcccatg tcctgtacgt
     4261 catcgcccgg gaccgggccc tcaacgacac ccagacgacc cggaccgtca cggtggagca
     4321 atccacggat atccccgcgg tgagcttgac ccatcccggg gcgggcgcct tcgtgggcgc
     4381 cggctacacg gtgcggggga ccgtgacgga cgacgacggc gtggcagcgg ccacggtcca
     4441 ggtgagctac cacgacggaa gctcctggag cgcctggggt tccgcgacgg ttacggggac
     4501 cgggcgggag ctgtccttca cctatgtcct gccggtcgga atcggggcgg acggccccaa
     4561 gcaggtacgg gcccgggccg atgacgacgc ggccgccaag ctggggggcg atccctcggc
     4621 ttcgggaacc tcttcggacg tatccttcac cctcgacacc cttcctccct cggtttccat
     4681 cctggtaccc gcggccgcct cggcccgcag gaccaccttc acggtctccg gagaggtggt
     4741 ggaacgcaac ctggataccc tgcgcctgag cctcgacggc gggccgcagg tcgacgcgcc
     4801 ggtcgtagga gtcggtgaga ccaagacctg gtcctatgac ctgcccggcg cggatttcga
     4861 cgccctcgcc gaaggccccc acacgatctc cgtggaagcg gtggaccggg tgggccggag
     4921 ccacacggtg cagcgggtgt tctacaagga cacctcggga ccggcgatat ccttcacctc
     4981 catcgtggag ggcgggaaca ccatcgtaac cgagacctcc ccggtactga ggggcgcctt
     5041 ctcggacgag tactccgaca tcgccggaac tttcgagtac cggctcgatt cctccctgga
     5101 tacggacccc tggaccaccc tggcggtcac gggtacgggc aaaaccgtcg catgggccgt
     5161 gtccgtggac gccttgtcgg acggaagcca taccgtcgac gtccggatcc tggacgcggt
     5221 cggaaacctc cgtaccaagg aggatgtggc cttccgcctg gaccgggccc ttcccgtcat
     5281 caacatctcg gcgccggttt ccgggggtat ctacggagcc gtcgcggcgg gtcccgtgtt
     5341 caccctttcc gggaccgcac aggacgccaa cctcgtctcg gtccgggcca agctgggctc
     5401 cgggccggag acggacatca ccggggtcgt cgcgggcggc accagcacct ggaactatcc
     5461 cgtggccaag gcggatttcg acgccctgcc cgacggaggc ggcaccgtga ccgtgacggc
     5521 cgtggacgcc gcggggcgga cgtcccagac ggcgtggacc ttcacgaagg acacgacgga
     5581 gccggacgcg gccttcaaca ccctggacct cggcggcacg acggtcctgc aggacagcca
     5641 accccgggtc ctgggaagct tctccgacgc gtacggcgtc gcctccatgg aaagccgggt
     5701 ggagcgctac gattatgccg gcaccctgtg gatcctggtg caggattgga cctccctggg
     5761 agctccgggc ggccaaaccg tcgccgcctg gtccaaggac ctgggaaccg cgggcctcaa
     5821 cctggccgac gggcggtacc ggatcgcggt ccgggcctcc gaccgggcgg tcccggacgc
     5881 gaacgtccgg acgaccgcgg ccgttgaatt ccggatcgac cggaacaacc cgtccctgac
     5941 cctgaatccc ggcggactct tccagaggac cgatttcccc ataagcggca cggcttcgga
     6001 cctgaacacc atcgcctcgg tgcgggccaa ggtcggggac ggggacttct cctccgggac
     6061 ggtgacgtca gcgtccacct ccaacggatt cgcgacctgg acggtcacgg taccgacagg
     6121 gggtctcact ccgggatcct atacggtgta cgtggaggcg gaggacgggg ccggcaggaa
     6181 aagccagctg acccgggagt tcagtttcga cggaacgccc ccggtcgtct ccatcagcga
     6241 gcccccgaac ggcagccgtg taaacggcct ggtgaccgtg cggggcacga cttcggatga
     6301 caatgccgtg gccctggtcc aatacaggat cgggaacggt gcgaccacct ggcagaccgc
     6361 gggattggga ggggggctct atagctggag ctacaccttc tcgaacatca acacctacgc
     6421 gaacacctcg gacgtcacgg aggtcgatcc cctgaccggc cttcccgcct cgggcacgaa
     6481 cgtctgggcc ctgcctttcc aggtccgggt cacggacgtg gcgggcaata ccacggaaga
     6541 actatcctac cggctcctgg tggaccccga catggacgcc cccgtggtga ccatcataag
     6601 tcccatgaac gaccagatcg tcggcggcga ggttcgggtt tccggctacg cttccgacga
     6661 cgactgggtg cggagcgtcg agcttcgggt cgaccccacc ggaacgggga cttccttcgg
     6721 ggcctgggag cccgcgacca tggtgaacca gggcacccag gtgaactggt tcaagaacat
     6781 caacacctcc ggggagctca accccgcggc cggaacgatc cgggacgtcc ggatccaggt
     6841 ccgtgcctac gattccaagg actacggggt gaccccgggg atcacggggg acacggtcga
     6901 gctgaacgtc aagttcgaca gcggagtgcc ggttatccag aacgtccggg tattccgaaa
     6961 cggttccgag gtcgagtacg cggcgggtgt ccgtgcggcg gggatcttct cggtccgggc
     7021 gagcgtccgg gacgagggag gcatcgccag catcaagtgg cgccgggaag gccagacggt
     7081 cttcacgaac atcctggccg atcccgcgta cacgacccct ccgacggaac ggaccgccgg
     7141 atttttcgaa gtcgggcgga agtacctgat cacctcggcg ggcacgacgg acttcacggc
     7201 gatcggagca agcagcaaca cccctgggat ctccttcctg gctacgggta tgggcgccgg
     7261 gaccggcacc gcctacgaag cgatcggacc cttggcggat cccaccaacc agtacttcca
     7321 gtacgaggtg gagatcgaga tcgactccac gacggtcaac gcggggcagt acgcgggcac
     7381 cacggggttc ttctcccttg acctgcaggc cacggacaac gcgaccccca cgccgtacct
     7441 gacccaggcc agcctgaaca tccagatcga caactacttc cccttcgggg agtacagctc
     7501 gtccgcgaga gcctccacgg cgaattacta catccagggc cgggcctggg attccgggac
     7561 cggatcggga agcatccagg gcatcgaccg tgtggtggtc tacttctaca agaacggggt
     7621 atacatcgcc cccgacggag gggctttcaa cggtaccacc cgtaccctca aggacatgac
     7681 cgcgggcgga accctccaga cggttgtatt ccctgcggac gccgcttcag ggatcgtcat
     7741 cgacggcaac gaggtgacct cggatcttga cggcagcggg tacattgaag gtttcaccga
     7801 cgacgggatc tacaagaatt ggtacgccat ttacgacacc actcaattgg gcgacggtcc
     7861 tatggacgtg cactacgtag tcgtagaccg ggccgggaac tcgacccggt atgtccaagc
     7921 agtctacatc cggaacaacg cgccccggat caccggcgtc gtcatcggca ccgatgtgga
     7981 cggaaacggg acggtcgggg acatcgcctc cggggagagt cgccggatcg cggcggccta
     8041 cgggacgacg aacttcacgg tccgcaacga acgtctggcc ttcgaggccg agacggcggg
     8101 cgggaatctt ccgaaccgct actccatcgg ctacgtgacc ggcactgcgt ccgtcggggc
     8161 cgcggcgctg gttcccggga gcgtctatac gatcgagacc gccggaacca caaactggac
     8221 cctcctgggc gccccggaca acctggcggg cacgaccttc gtggccggcg gctcgggtgc
     8281 cgggaccggc accgcgacgg gctacacgat cgtccagaag aacaccgcac cgtacacctc
     8341 ggctaccctg accctggacg atttcacggg catgcccgac acggcagcgg ccaacgaggc
     8401 gcgcttcgtg gtgaaggtct acgacacgac cgttgccgcg ggaacggagg cggaccagct
     8461 ggccgacgcc gtggtgatcg gcctcagtat cgacaatgtg gacgacctcg cgcccggcat
     8521 aacggctgca cccttcggcc gcgaatacac gtccccggcc agcttctccg acgcggacaa
     8581 ggcgctgaag gacgtggacg cctacacgga aaacctcgtc acctcaggca gcggggtctc
     8641 cctcgtgaaa cacgggtacg ctcagtacgc cctgcattcc ggggacgggg acgcggatgt
     8701 cagcggcaag gtcgtattcc tcggaaaggc ggcggacaac cagaggatcg cccggatcac
     8761 ggtccagatc cccggatata acgggggggc cgccttcgac atcgccctct gggacggatc
     8821 ggccctgtcc gcggccgcgg cgggtcggac cttggccgac gtggccgcgg ggacctcgga
     8881 ctggggcttc gaggcggagg ccggcagcca agccgtgacc gagacatacg gccacgtgct
     8941 caactggaag ttcgcctgga actccgcggc cctctccacc gtgaccgccc cggacgtcat
     9001 cgtgaccttt acggcctacg acgcgggacc ctcggggggc aacagctcct ccggggattt
     9061 gaccgtggac gtggtgccgt acatcacgaa gatctccaat ccgacggggc agggcggtct
     9121 gtcggatacg gtgatccgca gctcccgggg ccggtacagc atcgagtacc acgctacgaa
     9181 ccatttccgc gccagcggtt tcaacttgtc cggggcggtc gcccatgtca gctccgcggc
     9241 catcgacatc cagcccggga cgacgaatct caccgttgcc gcgacgacgg cgacgaccgt
     9301 cgatatacgg aagaacttct ctctctcggg ctacttgacg gtcttcgtga acggtatacc
     9361 cagcatcaac aatctgaacg ataccggcca ggcctaccac tccgaagccg ccgtgggaga
     9421 tccgcgttcg accttgtgga ccgatgatcg gtatctgacg gtttggaatc tgacccaggt
     9481 tctccctgcg gtcaccaacc agaccttcta ctacccgtcc atggtcatga acggaaacca
     9541 gccgcttttc agctacgcca acgacaacga cgggtatacc tataggacca cgggggtcgc
     9601 ggcctcatcc cagcggggag gcatctggtt cgagcgccac acggccttgg cccggtcggc
     9661 ggggggaatc tactggatcc tctcggtcca ggacgccgtc ggcaccgggg gcatcgggta
     9721 cctctacctc aaccgggacc ggaacgtgaa cgccctcctg ggcgacaccc agaacaacaa
     9781 ccacttcgaa atcataggtc tggattacac gagccggcag atgaaccgga tacggtatcc
     9841 caagctcatt gtcgagggac cggacaacgc gacgaacatc tacatctcgt actacgacgc
     9901 ccatccctcc cgccgtgaac tttccttcgt ctccttccag gcgtccacgg cgacggcctc
     9961 gaatctcact cagcccgcga acgacgcggc ccgggccgcc caggtccaag tcatcccgaa
    10021 cacggcgaac gccagcagcg agtacttcga catggtccgg atcggggcgg cggaggtcgc
    10081 ggtggcctac ttcgacgaga ccgccggagt cctgaagctc cagtattcgg gagacgcctg
    10141 gaacgcgaac aaccagagcg gagccgggac ctggacggag ttgaccgtgg acgacagcgt
    10201 cctgacggga tcccatgtgt ccatgaccgt cggccagagg agcggtacga cctacctcta
    10261 cctggcgtac cacgacgccg cggagacggc cctgaaattc gcgcagatcg attggaccac
    10321 gaaggccgtc acgaagtcgg tgatcgacaa ccggttctcc gtgggtactc ggaccaacgt
    10381 cttcgtgatc gatgagctgc cctacgtatc cttctattcc gattcgtaca gcggcacccg
    10441 gaattccatc cggctggcct accccgtggc cgccaacggc cgtacagcgg cggagaacat
    10501 cctgcagccc ggggccgacg tcaacgagga gtacacgggc gactgggaag tagccgcggt
    10561 accggccgtc acggtcccca aaggagggat cgagcagttc aatcgaaccc agttgaacca
    10621 gtacaccaac ggagcgtccg tcctgcccgt cgtcggctgg cttgcggatc ggatcgagta
    10681 cgccgagctc cagccgactg cgccgtgacg ggaggacgaa ggtgaaacca tccgtgcggc
    10741 gcactgacaa gcgggccgga aatcaccggc cgggaatccc gggtcccggg cgcttcaaga
    10801 tcatcgccgc ggcggccgcc cttgcggcgg tattggccgc gggctacctc ctgctccggc
    10861 cccggccggt cttcctggtg gaggagggtt tcgttcccgc ctgggagaag ctcctcgagg
    10921 agggcccggg tgcgcccggg tggcggatcg tgaagtacga tccccgggat gggctgccct
    10981 cgacccgagg tcttcgagga tatagaatct ccacggacag ggaaggtcgg ccgccggccg
    11041 gccggtccgc agcggccggg tcggcggagt ccgtccccat cccggtgccg cgccgggacc
    11101 tgtcccggaa cccggcgttg ccggacggag ccctgcccct ggcggtggac ccgtggatgg
    11161 tcttccgccg acacgccgac ccgcctccgt cgcgggccct cctggaaaaa ggcggatccg
    11221 gcgcggtcgt ccttcttccg gggggggagc ccgaggccct ctgggcctgg gccggccagg
    11281 aactgcaaaa tgtccccgga tcctttcctt cggaccccgg gctctggctg gatcgcacgg
    11341 accgaatggc ccgggacagg cgcttccagc ccggggccct caccgcgcgc tgggaggatg
    11401 tcctttcccg gctcctggaa ccgggcgcct cctggttgta cgcgcccctg agccgggtgc
    11461 tgactctgcc tggggaccgg acgagcctcc tggaggccga tcccttcccg ccccgggaag
    11521 gctggaacga tttcggcctg caaacccgca tcctctgggc cgtgcccctg ggtagaggga
    11581 cagcccgggg gttcatggct tccgcggccc gctggcttgg ggatccggcc atccaaaccc
    11641 gcctcggggc gatcctggac tgggctcccg cccatcggga cgcccgggcg gcaagtcccc
    11701 tggcgcggac ctcccaggag gcctggctaa aaagcaccta cgtgtgggag gctcccgaat
    11761 atgcgcacat cgactgactt ccgcccggcg cttccggctc gctttgcgac gcgctggatg
    11821 ctcgcggcgt tgatcctcgc ttcctccgtt cccgcatggg cgaacggcac ccccgaagag
    11881 acctggcagg aggcgacggg cacggaggta tggctccggg agttcgacct gtccggcgcc
    11941 aagcccggca cccacaacgt cgtcatccgg gcccgggact cggcgggaaa cgaggccctc
    12001 gtgggaccgt acaacatccg gatcgacccc tccgtgggct tgccgacggt ccgcatcatc
    12061 taccccgaga agggcaccgt cctgcgcagg gacgttcaca tcgtgggcgt ggcgggagga
    12121 cgcatcggaa tccaggccgt ggagatcaag ctcgacgggg gcgaatactc ccccgcgagt
    12181 ggctccgagt actggagcgc ctccgtggac gcggcgtccc tgccggacgg gcgtcatacc
    12241 ttgacggctc gggccgtgga tccccgaggt acctcgggac cggagacctc ggtgtccttc
    12301 atcctggacc gggcgcctcc ctcagtccgg atccggtccc acgagtccgg agccctgatc
    12361 cgcggggccg tccaattcga agggaccgcg gatgacgcga acggcatcgc ccggctctcc
    12421 ttgagcgtcg acggcgggaa gacgtaccag gacctcggtt tccggggcgc ccggggggag
    12481 acggaggtag ggttctcctt tcccgtccag agcggaaagc ttccggacgg agccgcgatc
    12541 tactacctcc gggcggagga cggaaccgga gccgtatcgg tcaaccccgt cctgttcttc
    12601 gtggacaaca aatccccgga gttggagatc ctgtggccgg aaaaaggcgc gaccgtcgcg
    12661 ggcaccttcc ggatcaccgg cacgatgaag gacgcggtgg gggttcaaag cctctcctgc
    12721 gaagtcggaa agtccgtgct ggaaatcgag atgcgtcccg gcgaccccta ctggacggcg
    12781 gacatcccgg tgcccccggg caccggacgt tccgtggacc tgcggatcgt cgcgatcgac
    12841 cggagcggca accggacctc ggtctcccgc agtttcgtga acgatctagg gtcctcgata
    12901 cccagcgtga agatatccca tcccggcgag gcgggactcg cgacgttccc ggcggacggg
    12961 gcgatcttcg ggtacgttct ggggggagcc ggtgtggacg ccgtaaaggt cgagggagga
    13021 aagacctacc ccgcccggcc gggcttccgc atcccctcct cggacatcgg gccgggccgc
    13081 agcgaactcc gcctccaggc atcgggaccc gaggcagccc cgggcgaggt gttgaaggtc
    13141 cgggtcgccc gtcccgccct gccttcggga accctgccgc cctcggcgcc gccgccgtcg
    13201 aatattacgg tgacctcccc ggcgccgtat tcctaccagc ggggggactt cagcatcgcc
    13261 ggtaccgccg aggcaccggc cggttcccgc ctggaatata ggacggctcc tacggagtca
    13321 tggcaggaaa taagcctggg cggggacggt aggttctcct cccgggtcgc cccggctgcg
    13381 ctgcccgagg gaccgatgca tctggaactt cggacggtga ccgacggaac cgcggccgag
    13441 ccggtctacc atccggtcaa ccggcacacc accgcgccgg agatccggat ccactccccc
    13501 cggtccgggg agaaggtgaa cggcagcgtg accgtctccg gggacgtcgt cacggccgcc
    13561 ccgatcgcgg atctttcgta taccctggac ggaaagacct tccgggccct ccagggcatc
    13621 cccgcctcct acggagccgc cttctccgtg atcgtggact tcacggtcct gcagaagtcc
    13681 gggggaagcg tcggcgtccg ggcggtggac gcggcgggaa actccgcgga tatcgggata
    13741 cccgtgaccg tggacgcgtc gggggacctg ccctcggtcc agctgaacct tcccgtggac
    13801 ggagccgtgg cgaccgggga tttcgacata tccggcatgg ccttcgacga cgacggggtg
    13861 gcggccgtcc attggcggat cggggaaggg gccttctccc gcctggacgt gaagcagagt
    13921 ttccggatcg gggttcccct ctcgtccgtc cgggacggag agcggagaat cgaggtctac
    13981 gcggaggacg tctacggccg ggtcgggccg cccgcgtcgg cggtcgtccg gatcagcacc
    14041 ggcgcgccga ccgtccaggt agtgtccccg agcatcgagg cgtacaaccg gggcaccgtg
    14101 atcctgcggg ggaccgcccg ggatccgaac ggcattttcg gggttcgggt ctccatggac
    14161 aacggcgcca ccttccagaa cgccgagggg accgagtcct ggagcctgtc cctcaacacc
    14221 ggagcgtaca aggacggggt gtattccgtc ctcgtggtgg cggcggattc cttcggtgtg
    14281 gagtcgagga tctcctccct cctcaacatc gacaacacgc cgcccctgat ttccctcggg
    14341 gagcccgcgg acggccgggt tacggaaccc aagcttcgag tttcgggtca gaccagcgac
    14401 aactcgggtg tcgagtcctt ggcctttgag atttcccccg tgtcggggtc tggagcccag
    14461 cgcggcctac ggatcgaggc cgagccgcgg ttggtgatcc tggaaaccct ggacgtctcc
    14521 tccctcccgg gggggaaata caatctccgg atgatcgcct ccgacgcggc gggcaacacg
    14581 tccgtcctgg tacggggcat cgaactcgta aaggaagcgg gggcccaccg ggtcgtcctt
    14641 ctggatcccc ttccgggggt ggcgcatacc ggcccgttga cggtttccgg ccgcgtgacc
    14701 ggtcccagcc tcccggcctc tgtcggcata ttcacgggcc agacgcgggc cgcggatgcg
    14761 gaggtcgacc ggttcggtta tttccggcac actctggagg gcatccccga ggagggccgc
    14821 ctggtcctct gggcgggctt cgacacttcc gcgggggaga aggtccggag cgcggaccat
    14881 gccgtcatcc tggaacccta cggccccgtc ctgacgatct tgagccacgg cgccggggac
    14941 gcggtgaccg gccgtccctg gctgagcggc acggcgggaa tccggctttc tcccggggag
    15001 gccgaagcgg cgggtaaaaa ggccgcgcgc tactacgagg tctcgaacgt ggcgattagc
    15061 ctggataacg gccggtcctt ccagccggct caggggacct cggaatggaa attccggctg
    15121 gaaaccgccg aactcccccg ggggcccctg cccgtgctgg tccgggccga gtacgcggac
    15181 ggacgtgccg tggtccgcag gatcctgctg accgtggata ccgttccccc cgtggtgacc
    15241 ctcgtggctc cccaggaagg aagcctccac gcggaatccc tcctgatgtt cggcaccgct
    15301 tcggacgatt acggcctgga gacattggag gtcaaccttc gcgcggggga caaggccggg
    15361 tatgcgctcc cccagttcat ccagggattg tacctggaca ctcatgccct gggcgcgacc
    15421 tacggggacg tgggcctggg ggtcagcttc ttcgacaaca acgtcaagct tcagctgcag
    15481 gcgggcatgg cgcccccggg ccgcttcacc ggtacggtgt tcggggccaa actcctggcc
    15541 aacgtgctct acctgccctt cgattacttc ctgggtccgg actggtcctt cttctccatg
    15601 tccctggccc tgggtgccaa cttctcctac ttcaccatgg acgccggcag cgatcccctg
    15661 gtcatgtcct cggtcctggt gcagtgggag ttcgcgcggt tccagtttcc cgagtggaag
    15721 gctttccgga ccttctccct gtacgcggaa ccgaacctct ggttcgccgc ctcggacgtg
    15781 gaggccggag ccatcttcaa gctggccttc ggattccgga tcggcatctt ttaaggtccc
    15841 tcgaacagcg gcccgcccct ccccggggcg ggccttttca tatccgggtc tctctacctc
    15901 cgtttaccgt tcaccgacca caggttacac tcgctccatg ctcctcaccc cgtccttcct
    15961 gtcggcggcc cgggactacc gcctgctcct ggaccggggc taccccacgg cggccagcct
    16021 caagctggtg ggggaccgct gggccctgga caaggcggcc cggacggtcc tctttagggg
    16081 aatcctgccg gaatccctgt ccgcggccat cgcggtccgg ctcgtccgag tcctgcctcc
    16141 cggcgcccgg ctggccgtgg acgggtacaa cgtcctcttt accctgtcca actaccgccg
    16201 gggtcacccc ctcttcctgg ggaccgacgg cctggtgcgg gacgcggggg gagcccacgg
    16261 gcggatccag gaccaaggcc ttttcctgga atgcgcccgg gccgcgacgg agaggctcgc
    16321 ggacctgggg gtcgcgtacg ccgaagtgta cctggacgcc ccggtctccg ggagcgggtc
    16381 ccacgcggcg gtcctccggg acctcctggc ccgggcgggc gtgacggggc gggtggagct
    16441 ggccgcttcc gcggacgggc cggtccggga cttcgaggga ggcgccgccg gctatgccgc
    16501 cggccaagcc gaaggctttg tcgccacctc ggactccgcg gtggctgtcg cggcccggtc
    16561 cccggtcttc gacctggccc gggacatcct ggaggcccgg tacagggcgg tcttcccgga
    16621 tctcggggtg gtggtacgaa gctcgtgaaa cgaaccgcgg gggatcgaag gccgcagggg
    16681 agaaccgttc cggcttgaca gccccttctc cgggggcgga tactttttca ttctattgcc
    16741 gcgccgaaaa ccctgaaggt ctccggcgga aggagccccc atgcagtccg aagccggaac
    16801 tctccacaaa accgtcctgg aggagatcga acgtgtccgt ccggatgccg tgcggatcag
    16861 ccgctccatc cacggccatc cggagctggg cctccaggag cgctgggctt cggccttcct
    16921 ggccgaggag gcccggaaac gcggcttcga ggtggaggcc cccgtcgcgg gcctttccac
    16981 ggccttcgcg gctcgataca cctccccgaa gccgggaccc aaggtgggct ttctctgcga
    17041 gtacgacgcc ctccccggct tggggcacgc ctgcggccac aacatcatcg ccgccgcgtc
    17101 ctgcgccgcc gccttcgccc tcaagaccct ggcggagaaa ctgggcggct cggtcttcct
    17161 cttcgggacc cccgacgagg aggcgatgag cgaggagtcc aagggcggca aggtgatcat
    17221 gagccgggcg ggcctcttcc gggacctgga cgcggtgttc atgacccacc ccgcgggagg
    17281 ctccgacgcg gcttggcgtt acaacttccc cctcaaggac ttcaccatcc ggttcaaggg
    17341 caggcccgcc cactacaccc agccggagaa ggggatcaac gccctggagg ccctcctcct
    17401 gtgcatcgcc aacctgaacg ccctgaaacg ggcctggtcc ccctcggtcc tcctggcgta
    17461 cacgatcatc gacggcgggg ctcccagcgc catcatcgtg cccgagaccg ccaaggccaa
    17521 gacccgggag gcgaaggcaa cgctcgaaca g
//
LOCUS       DAOTEI010000064        17339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2220_length_17339_cov_6.290673, whole genome shotgun sequence.
ACCESSION   DAOTEI010000064 DAOTEI010000000
VERSION     DAOTEI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 17339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17339
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2220_length_17339_cov_6.290673"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="P5313_07945"
     CDS             <1..1502
                     /locus_tag="P5313_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY80332.1"
                     /translation="MSQNTPPVYAGLDIAQACALELTAFDGFLAGLSIPGSEALVSKM
                     RSLAGRLIGMGAGYLSLDRAVSTLSGGESQRVKLARRLDCDLAGLLYVLDEPSSGLHP
                     ADARRVLAALRALSGAGNSVLVVEHDPALIEGADWAIEIGPAAGRGGGELLYAGPVGG
                     LAGTEGPTGRILRERAARRAFRAAAVPSGAGAAAPRVRRPWTRSFPVRDARANNLKGV
                     DVDIPLGILVGICGPAGSGKSTLIHEVFAPAHPEAVVVAQGAIGRTSRGTVASYVGAF
                     DLMRKEFAKATGADPGLFSFNSKGACPKCGGAGFLAVEMNFLDDVRMTCDECGGARYR
                     AEVLDIRYGDRNVADALAMTAEEAEGFFRTPELRRRLAVLREVGLDYLPLGQSLSSLS
                     GGESQRLKLASELHKTGGVYILDEPTSGLHPTDVARLVGVLERLASGGNTVVVIEHDL
                     DALAACSWLIELGPAGGAAGGRVIARGTPEDLAADPDSVTGPFLREVLG"
     gene            1634..2737
                     /locus_tag="P5313_07950"
     CDS             1634..2737
                     /locus_tag="P5313_07950"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY80333.1"
                     /translation="MNEPAPTRIVASALSSIVDRHEAEDLFSGSVLVARGDAILLHEA
                     RGLAHRGFSVPNTVETRFDTASLGKLFTAAAAFRLADRGLLGLDDRIRDLVPLEGTKI
                     PGNVTVRHCLTHSSGIADDADEEAGEDYEEIWKTRPCYRVRDCADFLPQFAYKDPVFP
                     AGTASRYNNCAYVLAGLAMEARTGLSFRELVRREVIEPAGLSRTGYFAKDTPEPGIAE
                     GYATVEGAHGKAAGFRKNIYAFPPIGSPDAGVLTTVEDLRRLFRAIEGEDFLSPDLRR
                     DFLTPRVDAFALRDGGLRKYGHCLEFDYDPAGTLVRYGKDGVNPGVAAIAMRYPAAGG
                     FVAVLANQDADVWALCRELAAAAGFAAAPGAKD"
     gene            2768..3808
                     /locus_tag="P5313_07955"
     CDS             2768..3808
                     /locus_tag="P5313_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80334.1"
                     /translation="MYKEKEFRERCAARGLGPEAVEQAVTWVRVLEAEAPGPDGTLAG
                     ASLAEVERHVERLVAQGEVRDERLMALARYFAAAGIDAVAIRLLAYLLPVGVLPAMAD
                     RLAELEGGPVRDRVMAGVEVPPTGAPPEVYPAATAAFVQALEAVLGAEKARRVLCWNV
                     HGIPAAAFAAEREAFLASASLEEWLAAFHGRKVRELERHAEDGTLWFEQRITPAVVDF
                     VRGNQEILSTVSDGRHLWATKIPYDPDRFLASQDPLEKRRLACHCPLAASSITEAGAG
                     VPSAWCACSAGYEKFLFDTVFGEETEAEVTESVLAGDPRCRFRIRIPDSVLERFRTPD
                     PPGSSRRAGGAA"
     gene            3805..4731
                     /locus_tag="P5313_07960"
     CDS             3805..4731
                     /locus_tag="P5313_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRY80335.1"
                     /translation="MRGRKDRGAGRPRSAGRLAAFVLLAAAAGTLGAQIPDPWRGPDA
                     RDLVEAVEAAMVNVEPGVFVMGSDRGLPDSRPAHSVALTKPFRILRHEVTFEEYDAYC
                     ALAGARSPKDNGWGRGRQPVIDVSFADAVRFCNFLSRAAGRTPCYDEEGRTFDPSADG
                     YRLPTEAEWEYAARGGPRSRGFRLSGSDDPSEVAVFGDAPSPAAVESKKPNELGIYDM
                     SGNVWEWCQDWYAAAWYSKAPAGSRDGSLPWADPAGPEIAEADRSYGVKRVRRGGNYH
                     ESADSAAVHFRSRDLPKQADPGMGFRIVRNGP"
     gene            4744..5427
                     /locus_tag="P5313_07965"
     CDS             4744..5427
                     /locus_tag="P5313_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRY80336.1"
                     /translation="MRIVPEGRCVRGRRTPGAADRLVSAAALFLAVLLAAGCASAVKP
                     TASASEPKKALYPWTALIVVDVQRGIFPLHREATFLAAVRTLVDRANEAGVPVIYVYH
                     TDGGSFTPGRESWLLHPSLTPPEGAEQVRKSTADSFSGTELEALLDARRIGRVVICGL
                     GSTACVSATLWGADGRGYDLVLPSDAHSMPLTGSSLEALERFNEFWKGNGAEVLPAAL
                     VDFKKTAGS"
     gene            5535..7169
                     /locus_tag="P5313_07970"
     CDS             5535..7169
                     /locus_tag="P5313_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRY80337.1"
                     /translation="MPDSIEALEALLKSRPDRALEGALVLLSETRGLPDPSRASHAAA
                     VAAYAALATGNPDRALELARQGQALKPVETGLRVRLLCAEGISHFQAGRYVESIAILE
                     KALRLSRRSGDRTSLVRSLGNLGIASLEAGRVEKAAAYLREALDAAGNAAIPGVPVEA
                     LRLQYAEALTLCGRPEDALGLIDGVMSDAEASGNAVVQAEARLGKGESLRSCGRRTES
                     LDTLEDALARANRGGFAMVGVRAAVSLVRARIEDGQLDEAEALAEEWLPRAAAGDLLR
                     YTELLRLRGRVRALRGRFRDAYEDSERAAVLERRSGRPSAAEASLNRERGTLRRKARR
                     LSERLEGWAEAVTHALADLIEARDEVTGQHIDRTREIVLILGERLIEEGAFPRLGPGS
                     LTTIARMAPLHDIGKIAVPDAVLQKPGPLDDAERSLMQRHVIVGREILLEASKHIRYD
                     PRVALAAEIAGYHHERWDGDGYPEGLAGNDIPLPARLVAMADVYDAIRSERPYKPALG
                     RDTAGGYILENSGRHFDPVIVEAFRDREPDIARLYP"
     gene            complement(7180..9144)
                     /locus_tag="P5313_07975"
     CDS             complement(7180..9144)
                     /locus_tag="P5313_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRY80338.1"
                     /translation="MNILSLDGISKNMGGTILFSGVSLGVEDRSRAGLVGRNGSGKST
                     LLKILAGMLEPDGGTVARRRNLAVALLPQLPRFAPGATLGSFLLEDESPAVRLVRDYE
                     ACAAAVGRGEKRHAELERLTRRMEEERGFVLEHAYASYCTELGLPGPEARMDTFSGGM
                     VKKAALARLLCSGADLALLDEPTNHLDVETIEWLEAKLGSAPFAFVVVTHDRYFLDAV
                     CSSIWEIDDEAVYGYPGGYTDFLARRRERWEALEKADARRKAILKIEMKWLNRGARAR
                     ATKSERRKELIRGLQASALVRPESMGEFSSASRRMGGKVLRLEGVSKSYGDRAVLAPF
                     RHEFGRGERAGVVGPNGSGKTTFLGIVSGRIAPDSGKAEPGPTVTFGCFDQTAAALDP
                     SATVLDYVREHAEQVRLGDGSVLSAERLLERFLFDRDMFPRALGALSGGELRRLQLVR
                     LLAEAPNFLLLDEPTNDLDIETIELLEDFLEDFSGCVLAVSHDRAFLDRVTRTTFVLD
                     GEGGVRSFPGTYSDWRVARELEEEERAAAARSADRGRGKDRGGPAAAPFGVPGNSNPS
                     ASSDAPAREAAESARRRKPSFAERREFETLLPEIEALEAEKAELEALFAAGGRDGQSL
                     EQASRRYAELDPLIHAKTARWDELAELVDR"
     gene            complement(9266..9898)
                     /locus_tag="P5313_07980"
     CDS             complement(9266..9898)
                     /locus_tag="P5313_07980"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRY80339.1"
                     /translation="MIEYVVSANIDDRILRRAADLLRKGGVVAFPTDTSWSLGCSIAS
                     KDGIAKLKRLVGREADGPLTVICSALQQVSELCELDTVSFRAIKRLVPGPYVFILPST
                     NRAAKDFDLRRSELGVRIPENPVPCALADALGLPLLSVTAKRTLLGMSPKDAEFPEEL
                     LFSGGWEIDGIPGIDLVLDPGEEQERRVSTVLDMRGGGIEVLRRGAGDYP"
     gene            complement(9905..10891)
                     /locus_tag="P5313_07985"
     CDS             complement(9905..10891)
                     /locus_tag="P5313_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY80340.1"
                     /translation="MSRLTDFLGGTELFKGMSGLELKAVAELLKRRECPRGTVVFREG
                     ERGQELFIVLSGKVGSFVTQSDGTRRRIYEFGPGRFFGEMAIVEGEPRSATCWAEEDT
                     TLLVLEGIDFYRLVFDYPMIGLKLLSSIVRVMVSWLNESSRFLNDLVRWGETARRRAV
                     EDALTGLFNRRFLEETLANRFTSGLGSGRRLALVMLDLDHFREINGSFGPAAGDATIR
                     AAGAAFRQALRDGDVAARLSGDEFAFLLPNTGLDEALAVAERLRKRAETVRLDFRPPA
                     GGPASKVRITASLGVAVAPDHADTPGALIDRSDRALYLAKEGGRNRVAGPKA"
     gene            complement(10888..11907)
                     /locus_tag="P5313_07990"
     CDS             complement(10888..11907)
                     /locus_tag="P5313_07990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY80341.1"
                     /translation="MPQASSGPAADPGLPSLLRKAEIFSSLLDDDLAYAASRAGVIRL
                     AKGARLFDAGQKAQRFYVVRSGGIRVFRSRPDGGEDEMALFASGDALGDFDFARGAEY
                     DASAEAVEPTELVVFPGKDLVFDDLARERPDAAARLLLRSLAMISGRLRATHKLISEN
                     APWVRELHRQAYEDPGTGLWSKAFVDEELPRYLVSPTSLVMIKPDRFKVLVDSRGHAA
                     GDEAMARIAGVLQSSVRALGRGWAVRVRSNETAVIAPGCGREVAEELARSIGEKIADL
                     EPVPASRGTPFFLFSASVAYSVWPADGEDWKELFVRSQDALMAAWGEGGARVVPVAPA
                     PGGAP"
     gene            complement(12094..14844)
                     /locus_tag="P5313_07995"
     CDS             complement(12094..14844)
                     /locus_tag="P5313_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80342.1"
                     /translation="MKRRTRAAALMLLSVLAFPVAADLHSPNRPLRTIRTEWFDITFP
                     PEARVQAERLASFADDAYREVAAFLGTEPRLRYPVILSPDADGPNGYYTVWPRRRIAL
                     FLRPSDPNDGFGYQADDLRALFRHELAHAVSLTIRPPAWDLAAAVFGDAAGISYYTTP
                     ASLAEGVSIAAEGMDGRGRAADPLTADELRRDAREGRFKTFWEAAGAWDRYPYGRVPY
                     SYGALFTRYLLAEYGEELYRELWRRMGEGLILPGLGDFLFVRGAFRRVYGLPLSEAWA
                     DFEGRMTPSEPAAEPERLTPPGLLTALAAGGGTVWWSDGAARRIRSLDARTGLPGTPL
                     PGDGTISRISVSPDGRRLLVSHSVEEGGAARLVLRSVDPETGESRVLPHRGLRDGAWA
                     GTDILAVRAELGAGSLVRIRDGRGEVLAPGGPGLTWAAPCASPEGRWIYALRREDGEV
                     SVVRFPASAEPDAAGPSGLELLALPEGFSAVRDLSVDGDGTLRFSWHDGEAYRTAEYR
                     EGVLRYPEAAYAGGTRRAAAAEGRVFFLGEYSEGVILCAVPEGTAWVETTASWLPDPG
                     AAKIGTAPDTSPSPESWPYSALPGLAPSVRYPTFRLSSEGLWGVGAGAAGGDAAETFS
                     WRAGALWIPDLGAADLELSVEVTAFPPVLVLSASDTFEPGGSGAVRTTRAGLALDRSR
                     SIYGGFEASTGAGAEVSWFTSLGPGEDPYGPRDAALAAGTASVSFGRWGFEIRKPSAS
                     RGAGIGLTLFGARVLPPGAPETLAGIEGTLRFRALPLALSLDLYGAAALSPGLSYGPD
                     GRIFSGFAGGASYPGYDAWSGAGSGPWYAYGEAAASPLVLEIQGRLGPVHLQRLVLSA
                     GVRGALADGEPLGSAFVRASLDFAPLLGVYARLRPRAWAEAEYAPDPDRVLVRWAVEL
                     PL"
     gene            complement(14841..15977)
                     /locus_tag="P5313_08000"
     CDS             complement(14841..15977)
                     /locus_tag="P5313_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80343.1"
                     /translation="MDTSSALSIRPVRSRRDFHDFVNLPRGLYRDDPAWVPPIWLAEP
                     GEYRPSGNPVLARSEHELFLARRGDLAVGRIVAYVDPRFNGYYRSRVGFFGAFECRDD
                     VEAARGLYAAAEAWMADRGMESSRGPIHPSAECWGLVTGGFDRPPVFLSPHNPAYYTA
                     LAEASGYEGVKDLLSYEADSGAGYRIPERFSRFAALHAARFPGLSTRRIDLGSLDRDA
                     LHILRILNEGVAENWGYVPVEADEMTTVVRKLRPFLDPDAVWFVEDGGVPVACCLGFP
                     DVNVAIRAMRGNLLPLGFLRLLSVRRRVRDYRLWGLAVLPAWQGRGLDVLLYMRLFEA
                     LAPKGVRLEANYILEDNLRIRNALEKLGMRPVKTYRVYEKALGR"
     gene            16086..16712
                     /locus_tag="P5313_08005"
     CDS             16086..16712
                     /locus_tag="P5313_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRY80344.1"
                     /translation="MLIVPYGPARTEIRVVNSRFIASLEPVDSAEAARAFLARIRSEF
                     PDASHHVPAYILGGGNSVTEYCSDDGEPSGTAGRPLLAVLKGSGLGDAAVVVTRYFGG
                     TLLGTGGLVKAYSEAGRAVLALARRASLVETGRFELDLPYRLFDRFQVLAEELGAVVA
                     ERSFGEDVRLAVDLPLSARGDFEAALTSLSSGAVKVRLESTRLARIPL"
     gene            complement(16729..>17339)
                     /locus_tag="P5313_08010"
     CDS             complement(16729..>17339)
                     /locus_tag="P5313_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80345.1"
                     /translation="PMDSNPPSRCETAKAHFTLKTFDSAYMDKLFGEIRSCVEAVSGM
                     TGASGEVTVLDEYRCTIPNLNLAAGLFRHLRALGADVENPLDSRRDLERQRYPGTSTD
                     FSDVSWEAPAIHGYCGIGAGLVLHTPEFAAAAASEAGDEGMIRAASAMALTAADILSD
                     PDYAARVRGEFEAYRQAGFRGVPGIPPDFVPFPREFAELFGA"
BASE COUNT         2582 a         6168 c         6262 g         2327 t
ORIGIN      
        1 tcatgagcca aaacaccccg cccgtctatg ccggcttgga catcgcccag gcctgcgccc
       61 tggagctcac ggccttcgac gggttcctgg cgggcctttc gatccccggc tccgaggccc
      121 tggtctccaa gatgcggagc ctggcgggcc ggctgatcgg catgggggcg ggctacctct
      181 ccctggaccg ggcggtctcc accctctccg gcggggagtc ccagcgggtc aagctggccc
      241 gccgcctgga ctgcgacctg gcgggcctgc tgtacgtcct ggacgagccg agctcgggcc
      301 tccaccccgc cgacgcccgg cgcgtcctgg cggccctgcg ggccctgagc ggggccggca
      361 attcggtcct ggtggtggag cacgatcccg ccctgatcga gggggccgac tgggcgatcg
      421 agatcgggcc ggccgcggga cgcgggggcg gcgagctcct ctacgcgggt ccggtgggcg
      481 gtctcgcggg tacggagggt cccaccggac ggatcctccg ggagcgggcg gcccggcgcg
      541 ccttccgggc ggcggccgtt ccctcgggcg caggcgcagc cgcaccccgg gtccggaggc
      601 cctggacccg gagcttcccg gtccgggacg cccgggcgaa caacctgaag ggtgtcgacg
      661 tggacatccc cctgggcatc ctggtcggga tctgcggccc cgcgggctcc ggcaagtcca
      721 ccttgatcca cgaggtcttc gcccccgccc acccggaggc ggtggtcgtg gcccaggggg
      781 ccatcggccg gacctcccgg ggcaccgtgg ccagctacgt cggggccttc gacctcatgc
      841 ggaaggagtt cgccaaggcc acgggagcgg accccgggct cttctccttc aattccaagg
      901 gcgcctgccc caagtgcggg ggcgcgggct tcctggcggt ggagatgaac ttcctggacg
      961 acgtgcgcat gacctgcgac gagtgcgggg gcgcccggta ccgggccgag gtcctggaca
     1021 tccggtacgg ggacaggaac gtggcggacg ccctggcgat gacggcggag gaggcggagg
     1081 gcttcttccg caccccggag ctccgccgcc gcctcgcggt tctccgggag gtcgggctgg
     1141 actacctgcc cctgggacag agcctctcga gcctctcggg aggggagtcc cagcggctca
     1201 agctggcctc ggagctgcac aagaccgggg gggtctacat cctggacgag ccgacctcgg
     1261 ggctccaccc gacggacgtg gcccggctgg tcggggtcct ggaacgcctg gcctcggggg
     1321 gaaacacggt ggtcgtgatc gagcacgacc tggacgccct ggcggcctgc tcctggctca
     1381 tcgagctggg acccgcgggg ggcgcggcgg ggggccgggt catcgcccgg ggcaccccgg
     1441 aggacctggc cgccgacccg gacagcgtca cgggtccctt cctgcgggag gtcctgggat
     1501 gaacgacggc cgcccggggg ttcccgggcg gcgagccccg cgcggtcgcg cggctttttc
     1561 tccttccggc cctcccaaaa cgggggtata ctcatccgga tcattccaaa actgtcgcct
     1621 gcaggggtat cccatgaacg agccggcccc gacccggatc gtcgcctccg cgctgtcctc
     1681 catcgtggac cgccacgaag cggaggatct cttctccggt tccgtcctcg tcgcccgggg
     1741 ggacgcgatc ctcctccacg aggcccgagg cttggcccac cggggcttct cggtgcccaa
     1801 caccgtcgaa acgcgcttcg acaccgcctc cctgggcaag ctcttcacgg ccgccgcggc
     1861 cttccgcctg gcggaccggg gcttgctcgg actggacgac cggatccggg acctcgtccc
     1921 cctggaaggt accaagattc cggggaacgt cacggtccgc cactgcctga cccatagctc
     1981 cgggatcgcg gacgacgcgg acgaggaagc cggggaggac tacgaggaga tctggaaaac
     2041 ccggccctgc taccgggtcc gggactgcgc ggacttcctg ccccagttcg cctacaagga
     2101 tcccgtgttc cccgcgggca cggcgtcccg gtacaacaac tgcgcctacg tgctggcggg
     2161 cctcgcgatg gaggcccgga cggggctctc cttccgggaa ctcgtccggc gggaagtgat
     2221 cgagcccgcg ggcctgtccc gcaccggcta tttcgccaag gacaccccgg agcccgggat
     2281 cgccgaaggg tacgctacgg tcgagggcgc tcacgggaag gccgcggggt tccgcaagaa
     2341 catctacgcc ttcccgccca taggatcccc ggacgcgggc gtcctgacca ccgtcgagga
     2401 tctccggcgg ctcttccggg cgatcgaggg cgaggacttc ctgtccccgg atctgcgccg
     2461 ggacttcctg accccccggg tcgatgcctt cgccctccgg gacggcggcc tgcggaagta
     2521 cggacactgc ctggagttcg actacgatcc cgcgggcacc ctggtccgtt acggcaagga
     2581 cggggtcaat cccggggtcg cggcgatagc catgcggtac ccggccgcgg ggggcttcgt
     2641 cgcggtcctg gccaaccagg acgcggacgt ctgggccctc tgccgggagc tggccgcggc
     2701 cgcgggcttc gcggcagcgc ccggggcgaa ggactgaggg gccgcccggg ccccaaagga
     2761 ggcgaccgtg tacaaggaga aggaattccg cgaacgctgc gccgcccggg gcctgggccc
     2821 ggaggcggtg gaacaagccg tgacctgggt ccgggtcctg gaggcggagg ccccgggccc
     2881 ggacggcacc ctggccggcg cttccctcgc ggaggtcgaa cgccacgtgg agcgtctggt
     2941 agcacagggc gaagtccggg acgagcggtt gatggccctg gcgcggtact tcgccgcggc
     3001 cgggatcgac gcggtcgcca tccgcctcct ggcctacctc ctgcccgtgg gtgtccttcc
     3061 cgccatggcg gaccgcctgg ccgagctcga gggcggcccg gtgagggacc gggtgatggc
     3121 gggcgtcgag gtccccccga cgggagcccc cccggaggtc taccccgcgg ccacggcggc
     3181 cttcgtgcag gccctggaag cggtgctggg cgccgagaag gcccgccggg tcctctgctg
     3241 gaacgtccac ggcatacccg ccgcggcctt cgccgcggaa cgggaggcct tcctcgcctc
     3301 ggcctccctg gaggaatggc tggcagcctt ccacggccgg aaggtccggg aactcgagcg
     3361 ccacgccgag gacgggaccc tctggttcga gcagaggatc acccccgcgg tggtggactt
     3421 cgtccggggg aaccaggaaa tcctgtccac ggtgtccgac ggccgccacc tctgggccac
     3481 caagatcccc tacgacccgg accgtttcct ggcgtcgcag gatcccctgg aaaaacgccg
     3541 cctggcctgc cactgccccc tggccgcgtc ctccatcacc gaggccgggg cgggggtccc
     3601 gtcggcctgg tgcgcctgtt cggcggggta cgagaagttc ctcttcgaca cggtcttcgg
     3661 cgaggagacc gaggcggagg tgaccgagtc cgtcctggcg ggggatcccc ggtgccggtt
     3721 caggatccgg attcccgatt ccgtgctcga gcggttccgg acgccggatc cgccggggag
     3781 ctcccggcgg gcgggcgggg cggcatgaga gggcggaagg atcgcggggc cggccggccg
     3841 aggtccgccg gccgcctcgc cgccttcgtc cttctggccg cggctgcggg aaccctcggg
     3901 gcccagatcc cggacccgtg gagggggccg gacgcccggg acctcgtgga ggccgtggag
     3961 gccgcgatgg tgaacgtgga gcccggggtc ttcgtcatgg gcagcgaccg tgggcttccg
     4021 gactcccggc cggcccattc cgtggccctc accaagccct tccgcatcct ccggcacgag
     4081 gtgaccttcg aggagtacga cgcctactgc gccctggccg gggcgcggag ccccaaagac
     4141 aacggatggg gacgcggccg gcagccggtc atcgacgtct ccttcgcgga cgcggtccgg
     4201 ttctgcaact tcctgagccg ggcggcgggc aggaccccct gctacgacga ggagggcagg
     4261 accttcgacc cctccgcgga cggctaccgc ctgcccaccg aggccgagtg ggagtacgcc
     4321 gcccggggcg gtccccggag ccggggcttc cgcctttcgg gttccgacga cccctcggag
     4381 gtcgcggtct tcggggacgc cccgagcccg gcggccgtgg agtcgaagaa gcccaacgag
     4441 ctcggaatct acgacatgag cgggaacgtc tgggagtggt gccaggactg gtacgccgcg
     4501 gcctggtatt cgaaggcccc cgcgggctcc cgggacggca gccttccctg ggcggatccg
     4561 gccggtccgg agatcgcgga ggcggaccgg tcctacgggg tgaaacgagt gcggagggga
     4621 ggcaactatc acgagagcgc cgactccgcg gcggtccatt tccggagccg ggacctgccc
     4681 aagcaggcgg acccgggcat gggattccgg atcgtgcgca acggtccgta aaggaggtac
     4741 ccgatgagga tagtacccga aggtcgatgc gtacggggcc gccgcacccc gggcgcggcg
     4801 gatcggctcg tttccgcggc cgccctgttc ctggcggtcc tgctggccgc cggctgtgcg
     4861 agcgcggtta agccgacggc ctcggcgtcg gagcccaaga aggccctcta tccctggacc
     4921 gccctgatcg tcgtggacgt ccaacggggc atcttccccc tgcatcggga ggcgaccttc
     4981 ctggcggcgg tgcggaccct ggtggaccgg gcgaacgagg cgggagttcc ggtgatctac
     5041 gtgtaccaca ccgacggggg atccttcact ccgggcagag aatcctggct cctgcatccc
     5101 tccctgaccc ccccggaggg agccgagcag gtgcggaagt ccacggcgga ttccttcagc
     5161 gggaccgaat tggaggccct cctagacgcc cggcgcatcg gccgggtggt gatctgcggc
     5221 ctgggatcca cggcctgcgt aagcgccacc ctctggggcg ccgacggcag gggctacgac
     5281 ctggtcctcc cctcggacgc ccactccatg cccctgaccg ggtccagcct ggaggccctc
     5341 gagcggttca acgagttctg gaagggaaac ggggccgaag tactgcccgc ggcactggtt
     5401 gatttcaaga agaccgcggg atcctgacct tcgcccccga gccggagccg gggttcccga
     5461 cgttcggatt tgacacgccg cgggggaagc gcaagactct aggaggataa ccgcccgcgt
     5521 ccccggggga ttgcatgccg gattccatcg aagccctgga agccctactc aagtcccggc
     5581 cggaccgagc cctggagggg gctcttgtcc tgctgtcgga gactcgcggg ctccccgacc
     5641 cgtcaagggc gtcgcacgcg gccgccgtcg cggcctacgc ggcgcttgcg acggggaatc
     5701 ccgaccgggc cctggagctg gcgcggcagg gacaggccct caagcccgtg gagacgggcc
     5761 tccgggtgag actcctgtgc gcggagggga tatcccattt ccaggcgggt cggtacgtcg
     5821 aatccatcgc gatcctggaa aaggccctgc gcctttcccg taggtccggg gaccgtacct
     5881 ccctggtacg cagcctgggc aacctgggca tcgccagcct cgaagccggc agggtggaga
     5941 aagcggccgc ctatcttcgg gaagccctgg acgccgcggg gaacgccgcg attcccgggg
     6001 ttcccgtcga agccctgcgc ctccagtacg cggaagccct caccctgtgc ggacggcccg
     6061 aggatgccct gggtctcatc gacggcgtga tgtccgacgc cgaggcttcc ggcaacgccg
     6121 tcgtccaggc ggaagcccgc ctcgggaagg gggaatccct ccggtcctgc ggccgccgga
     6181 cggaaagcct cgacaccctc gaggatgccc tggcccgggc gaaccggggc ggcttcgcca
     6241 tggtcggagt gcgggccgcg gtctccctgg tgcgggcgcg gatcgaggac ggacagctcg
     6301 acgaggcgga agccctggcg gaggaatggc tgccccgggc cgcagccggg gaccttctcc
     6361 gctacaccga actcctgcgc ctccggggcc gggtccgggc tctgaggggc cgcttccggg
     6421 acgcctacga ggattcggag agggccgctg tgctggagcg gaggagcggc cgaccctccg
     6481 ccgcggaagc cagcctgaac cgggagcggg gcaccctccg acgcaaggcc cgccgcctgt
     6541 ccgaacggct ggagggctgg gccgaggccg tgacccacgc cctggcggac ctgatcgagg
     6601 cccgggacga ggtgacggga cagcatatcg accggacccg ggagatcgtc ctgatcctcg
     6661 gggaacggct catcgaggaa ggggcgttcc cccggctcgg cccgggaagc ctcacgacca
     6721 tcgcccgcat ggctccgctc catgacatcg ggaagatcgc cgtccccgac gcggtgctcc
     6781 agaagcccgg tcccctggac gacgcggaga ggtccctgat gcagcggcac gtcatcgtgg
     6841 gacgggagat cctcctggag gcgtcgaagc acatccggta cgatccccgg gtggccctgg
     6901 ccgcggaaat cgccgggtac caccacgagc gatgggacgg ggacggctat cccgaaggtc
     6961 tcgcgggaaa cgacattccc ctgccggcgc ggctggtggc catggcggac gtctacgacg
     7021 ccatccggtc cgagcggccc tacaaacccg ccctcgggcg ggacacggcc ggcggataca
     7081 tcctggagaa ttcaggccgt cacttcgacc cggtcatcgt ggaggcgttc cgggatcggg
     7141 agcccgacat cgcccgcctc tacccctgat cgggcgcctt caacggtcga cgagttccgc
     7201 cagctcgtcc cagcgggcgg tcttggcgtg aatgagagga tcgagctcgg catagcgccg
     7261 ggatgcctgt tccagggact gcccgtcccg tccgcccgcg gcgaagaggg cctccagctc
     7321 ggccttttcc gcctccaggg cctcgatctc gggcaggagg gtctcgaact cgcggcgctc
     7381 cgcgaagctg ggcttgcggc ggcgggcgga ttcggcggcc tcccgggccg gtgcgtcgga
     7441 ggacgcggaa ggattcgagt ttcccgggac gccgaaggga gcggccgcgg gccctccccg
     7501 gtccttcccg cgcccccggt ccgcggagcg ggcggccgcg gccctctcct cctcctccag
     7561 ctcccgggcg acccgccagt cggaataggt acccgggaag ctccgcaccc ctccctcacc
     7621 gtccaggacg aaggtggtcc gggtgacccg gtccaggaag gcccggtcgt gggagaccgc
     7681 caggacgcag ccggagaaat cctccaggaa gtcctcgagc agctcgatgg tctcgatgtc
     7741 caggtcgttc gtgggttcgt ccaggaggag gaagttcggg gcctccgcca ggagccgtac
     7801 cagctggagc cggcggagct ccccgccgga cagggcgccc agagcccggg ggaacatgtc
     7861 ccggtcgaag aggaagcgct ccaggaggcg ctccgcggag aggaccgatc cgtcccccag
     7921 gcggacctgc tcggcgtgtt cccggacgta gtccaggacc gtggccgagg ggtccagggc
     7981 cgcggcggtc tggtcgaagc agccgaaggt caccgtggga cccggttcgg ccttgcccga
     8041 gtcgggggcg atcctgcccg agacgatccc caggaaggtc gtcttgcccg agccgttggg
     8101 gcccacgacc ccggcccgct cgccccggcc gaactcgtgc cggaaggggg cgagtaccgc
     8161 gcggtccccg taggacttgg aaaccccctc gagcctgagg accttgccgc ccatgcggcg
     8221 ggaggccgag gagaactccc ccatgctctc gggccgaacg agggccgagg cctggaggcc
     8281 ccggatcagc tccttgcggc gctcgctctt ggtggcccgg gcccgggcgc cccggttgag
     8341 ccacttcatc tcgatcttga ggatggcctt ccggcgggcg tccgcctttt ccaaggcctc
     8401 ccatcgctcc cgccgccggg ccaggaagtc cgtatagccc ccggggtagc cgtacacggc
     8461 ctcgtcgtcg atctcccaga tggaggagca gaccgcgtcc aggaagtacc ggtcgtgggt
     8521 caccacaacg aaggcgaagg gggccgagcc gagcttggcc tccagccact cgatcgtctc
     8581 gacatccagg tggttcgtgg gctcgtccag gagggccagg tccgccccgg agcagaggag
     8641 ccgggccagg gcggcctttt tcaccatgcc cccggaaaag gtgtccatgc gggcctcggg
     8701 cccgggcagg cccagctcgg tgcagtagga tgcgtaggcg tgctccagga cgaagccccg
     8761 ctcctcctcc atgcgacggg tcaggcgctc gagctccgcg tgccgcttct ccccgcggcc
     8821 caccgcggcg gcgcaggcct cgtagtcccg gaccagacgt accgccgggg actcgtcctc
     8881 gaggaggaag gaacccaggg tggcccccgg ggcgaagcgg gggagctggg gcaggagggc
     8941 cacggccagg ttccgacgcc gggcgacggt ccccccgtcg ggctccagca tgcccgccag
     9001 aattttgagg agcgtagact ttccgctccc gttgcggccc accaggcccg cccgggaacg
     9061 gtcctccacg cctagggata ccccggagaa gaggatggtt ccgcccatgt tcttggatat
     9121 gccgtccagg gagaggatgt tcatgcgggg tggactgtat accgaagggg ccgtccgccg
     9181 caaggcggct cgccgcgggc ggcgctacaa ggcggcccgg ggccggaggg ccggatccgg
     9241 gacgaaggtc cggatgacgg ccggactagg ggtagtcccc ggcgccgcgg cgaagcacct
     9301 cgatcccccc gccccgcatg tccaggaccg tggaaacccg gcgctcctgc tcctccccgg
     9361 ggtctaggac caggtcgatc ccggggatcc cgtcgatctc ccagcccccg gagaagagga
     9421 gctcctccgg gaactccgcg tccttggggc tcatgcccaa aagggtacgc ttcgccgtca
     9481 cggacaaaag gggcaggccc agggcgtccg ccaaggcgca gggcacgggg ttctccggga
     9541 tgcggacccc caactcgctg cggcggagat caaagtcctt ggccgcccga ttcgtggagg
     9601 ggaggatgaa gacgtagggt cccgggacca ggcgcttgat ggcccggaag gagaccgtgt
     9661 ccagctcgca gagctcggag acctgctgga gggccgagca gatcaccgtg agggggccgt
     9721 ccgcctcccg ccccaccaga cgcttgagct tggcgatgcc gtccttggag gcgatggagc
     9781 agcccaggct ccagctggtg tccgtcggga aggcgaccac ccctcccttg cgcaggaggt
     9841 ccgcggcgcg tcggaggatg cggtcgtcga tattggcgga gaccacgtac tcgatcatgt
     9901 ttcgctacgc cttcggcccg gccacgcggt tgcggccgcc ttccttggcc aggtagaggg
     9961 ccctgtccga gcggtcgatc aaagcgcccg gggtatccgc gtggtccgga gccacggcca
    10021 cccccaggct ggcggtgatg cggaccttgg aggcggggcc gcccgcgggg ggccggaaat
    10081 cgaggcggac cgtctccgcc cgcttccgca ggcgctccgc cacggccagg gcctcgtcca
    10141 gccccgtgtt gggcaggagg aaggcgaact cgtccccgga cagacgggcg gccacatccc
    10201 cgtcccgaag ggcctgtcgg aaggccgcgc ccgcggcccg gatcgtggcg tccccggcgg
    10261 cgggcccgaa ggagccgttg atctcccgga agtggtccag gtccagcatg accagggcaa
    10321 ggcggcgccc ggagcccagg ccggaggtga agcggttggc cagggtctcc tccaggaagc
    10381 gccggttgaa gagccccgtg agggcgtcct ccacggcccg gcgccgggcc gtctcgcccc
    10441 accggaccag gtcgttcagg aagcgggagg actcgttcag ccaggacacc atcacccgga
    10501 cgatggagga caaaagcttg aggccgatca tcgggtagtc gaagactagg cggtagaagt
    10561 cgatgccctc caggaccagg agggtcgtgt cctcctcggc ccagcaggtg gccgaacggg
    10621 gctccccctc cacgatggcc atctccccga agaagcggcc cggcccgaac tcgtagatcc
    10681 tgcgccgggt cccgtccgac tgggtcacga aggagccgac cttgccggac aggacgatga
    10741 agagctcctg gccccgctcg ccctcccgga agaccacggt gccccggggg cactcccggc
    10801 gcttcaggag ctccgcgacc gccttgagct ccaggccgct catccccttg aagagctcgg
    10861 tgccccccag gaagtccgtc aggcggctca cggagctccc ccgggagcgg gcgccaccgg
    10921 gacgacccgg gctcctccct cgccccaggc ggccatcagg gcgtcctggg agcgtacgaa
    10981 gagctccttc caatcctcgc cgtccgcggg ccagacggag tacgccacgg aggcggagaa
    11041 caggaagaag ggggtccccc gggacgcggg gacgggctcc aggtccgcga tcttctcccc
    11101 tatggaccgg gccagttcct cggccacctc gcggccgcag cccggggcga tgacggcggt
    11161 ctcgttggag cgcacccgga ccgcccagcc ccggcccagg gcccggaccg aggactggag
    11221 gacacccgcg atccgggcca tggcctcgtc cccggcggcg tggccgcggg aatccaccag
    11281 gaccttgaag cggtccggct tgatcatgac cagggaagtg ggagagacaa ggtagcgggg
    11341 gagctcctcg tccacgaagg ccttggacca gagccccgta cccgggtcct cgtaggcctg
    11401 ccggtggagc tcccgcaccc agggggcgtt ttcggagatc agcttgtggg tggcccggag
    11461 gcggcctgag atcatggcca gggagcggag gaggagacgg gcggcggcgt cgggtctctc
    11521 ccgggccaag tcgtcgaaga ccaggtcctt gcccgggaaa accacgagct ccgtgggctc
    11581 caccgcctcg gcgctggcgt cgtactcggc gccccgggcg aagtcgaagt cccccagggc
    11641 gtccccggag gcgaagaggg ccatctcgtc ctctcccccg tcgggacggg agcggaagac
    11701 ccggatccct ccggaccgga ccacgtagaa gcgctgggct ttctgcccgg cgtcgaagag
    11761 ccgggcgccc ttggccaaac ggatcacgcc ggcccgggag gccgcgtagg ccaagtcgtc
    11821 gtccagcagg gatgagaaga tctccgcctt tcggagcagg gagggaagcc ccgggtccgc
    11881 ggcgggcccc gaggaagcct gcggcataat ccggtcctcc tttcggggag tataggggcg
    11941 cggggagggc ggggtcaata atgggacggt aaatagatga aggggtgaat acgtgaatgg
    12001 ggaggattca aacggacccc cggctacgtc ctaaccaccc caactgtaca ggccgttcct
    12061 attcactatt cactattcac caattcactt ccctcacaac ggcagctcca ccgcccacct
    12121 caccagcacc cggtccggat ccggcgcgta ctccgcctcc gcccaggccc gggggcggag
    12181 ccgggcgtag acgcccagga ggggggcgaa gtccagggag gcccggacga aggccgaccc
    12241 gaggggctcc ccgtccgcca aggctccccg gacccccgcg gagaggacga gtcgctgaag
    12301 gtggacgggt cccaatcgac cctggatctc cagaaccaag ggcgacgccg cggcctcccc
    12361 gtaggcgtac catggaccgg atccggctcc gctccaggca tcgtacccgg ggtacgacgc
    12421 cccgcccgcg aagcccgaga agatccggcc gtccgggccg taggagagcc cgggggagag
    12481 ggcggccgca ccgtacaggt ccagggagag agccaggggc agggcacgga atcggagggt
    12541 gccctcgatt cccgccaggg tctccggtgc ccccgggggc aggacccggg ctccgaacag
    12601 ggtcagcccg atcccggctc cccgcgaggc cgagggcttc ctgatttcga agccccatcg
    12661 gccgaaggac accgaggcgg tcccggccgc cagggcggcg tcccgggggc cgtaggggtc
    12721 ctccccgggt cccagggacg tgaaccatga gacctcggcg ccggcgcccg tcgacgcctc
    12781 gaagcctccg tagatggatc ggctccggtc cagggcaagg cccgcccggg tagtccggac
    12841 cgccccggag ccgccgggct cgaaggtgtc cgaggcggac aggaccagga ccgggggaaa
    12901 ggccgtcact tccacggaaa gctccaggtc cgcggccccc aggtccggga tccagagggc
    12961 gccggcccgc caggaaaagg tttccgccgc gtctcccccc gcggccccgg ccccgacccc
    13021 ccagagcccc tccgaggaga ggcggaacgt gggataccgg acgctcggtg ccaggccggg
    13081 aagggcggaa taaggccagg actccgggga aggcgaagtg tccggagccg tcccgatctt
    13141 cgcagcacca gggtcgggaa gccaggaagc ggtggtttcc acccaggccg ttccttccgg
    13201 aaccgcgcac aggatcactc cctcggagta ctcccccagg aagaagactc gcccctccgc
    13261 ggccgccgcc cggcgcgtcc ccccggcgta ggccgcctcc ggataccgca ggacgccctc
    13321 cctatactcc gcggtccggt aggcttcccc gtcgtgccag gagaaccgga gggttccgtc
    13381 cccgtcgacc gagaggtccc gcacggccga aaagccctcg ggcagggcca ggagctcgag
    13441 cccggacggg ccggcggcgt cgggttccgc ggaggcgggg aagcggacga ccgaaacctc
    13501 cccgtcttcc cggcgcaggg cgtagatcca acggccctcc ggggaggcgc agggggcagc
    13561 ccaggtaagc cccgggccgc cgggcgccag gacctccccc cggccgtccc ggatccggac
    13621 cagggagccg gccccgagct ccgcccggac ggccaggatg tccgtcccgg cccaggcccc
    13681 gtcccggagc ccccggtgcg gcaggacccg ggactccccg gtctccggat cgaccgagcg
    13741 gaggacgagc cgggccgccc cgccctcctc gaccgagtgg gagaccagga ggcgccgccc
    13801 gtcgggggac acggagatcc gggagatcgt cccgtccccg ggcagggggg tgccgggaag
    13861 gccggtccgg gcgtccaggg accggatgcg ccgggccgcc ccgtcggacc accagaccgt
    13921 cccgcccccg gccgccaggg ccgtcagcaa gcccggcggg gtcaggcgct ccggctccgc
    13981 cgccggttcc gacggggtca tgcgcccctc gaagtccgcc caggcctcgg acaggggaag
    14041 gccgtacacc cgccggaagg cgccccggac gaagaggaag tcccccagcc ccggcaggat
    14101 gaggccttcg cccatccggc gccagagctc ccggtagagc tcctcaccgt actccgcaag
    14161 gaggtaccgg gtgaagagag ccccgtagga gtagggaacc cggccgtagg ggtagcggtc
    14221 ccaggccccc gcggcttccc agaaggtctt gaaccgcccc tcccgggcgt cccggcggag
    14281 ctcgtccgcg gtcaggggat ccgcggcccg tccccggccg tccatcccct ccgcggcgat
    14341 ggaaaccccc tcggccaggc tggccggggt cgtgtagtac gagatccccg ccgcgtctcc
    14401 gaagactgcc gccgccaggt cccaggcggg cggccgtatc gtcagggaga ccgcgtgggc
    14461 cagttcgtgg cggaagaggg cccgaaggtc gtcggcctgg tacccgaagc cgtcgttcgg
    14521 gtccgaaggc cggaggaaga gcgcgatcct gcggcggggc caaacggtgt aatacccgtt
    14581 cgggccgtcc gcgtcggggc tcaggatcac cgggtaccgg aggcggggct cggtgcccag
    14641 gaaggccgcc acctcccggt acgcgtcgtc cgcgaaggag gccagccgct cggcctggac
    14701 gcgggcctcc gggggaaagg tgatgtcgaa ccactcggtg cggatcgtcc ggaggggtcg
    14761 gttcggggag tggagatccg ccgcgaccgg gaaggccagg accgaaagga gcatgagggc
    14821 tgccgcccgg gttcggcgtt tcatcggccc agggccttct cgtagacccg gtaggtcttg
    14881 accggccgca tccccagctt ctccagggcg ttccggatcc gcaggttgtc ctccaggatg
    14941 tagttggcct ccaggcgcac ccccttgggg gccagggcct cgaagagccg catatagaga
    15001 agcacgtcca ggccccggcc ctgccaggcc gggaggacgg ccaggcccca gaggcggtag
    15061 tcccggaccc gccgccggac ggacaggagg cgcaggaagc ccagggggag gaggtttccg
    15121 cgcatcgccc ggatcgcgac gttcacgtcc gggaagccca ggcagcaggc caccggaacg
    15181 cccccgtcct ccacgaacca gaccgcgtcg ggatccagga aggggcggag cttccgaacc
    15241 accgtggtca tctcgtccgc ctccacgggt acgtagcccc agttctccgc gaccccctcg
    15301 ttcaggatgc gcaggatgtg cagggcgtcc cggtccaggg aaccgaggtc gatgcgtcgg
    15361 gtcgagaggc ccgggaagcg ggcggcgtgg agggccgcga accgggagaa ccgctcgggg
    15421 atccggtagc ccgctccgga gtccgcctcg taggacagga ggtccttgac tccctcgtag
    15481 cccgacgcct ccgccaaggc ggtgtaatac gccgggttgt ggggggagag gaagaccggg
    15541 ggccggtcga agcccccggt cacgaggccc cagcactccg cggagggatg gatcggccct
    15601 cgggaagatt ccatgccccg gtccgccatc cacgcctccg cggccgcgta gagcccccgg
    15661 gccgcctcga cgtcgtcccg gcactcgaag gccccgaaga agccgacccg ggaacggtag
    15721 tagccgttga accggggatc gacgtaggcg acgatccggc cgaccgcgag gtccccccgc
    15781 cgggccagga agagctcgtg ctccgaccgg gccagcacgg ggttgcccga gggacggtac
    15841 tcccccggct cggccagcca aatcgggggg acccacgcgg gatcgtcccg gtagagcccc
    15901 cggggcaggt tcacaaagtc atgaaaatcc cgccgggacc gcaccggccg gatcgacaac
    15961 gccgaggatg tatccatggg gacattctcc ggcaccgcct gggaatccgc aagtccgccc
    16021 gagcctcgtt cgcggctcga aaccccgtcc cggcgtcgcg tccgacaatc tgctatactc
    16081 cccccatgct catcgtcccc tacgggcccg cccggacgga gatccgggtc gtcaactccc
    16141 gcttcatcgc gagcctggag cccgtcgatt ccgccgaggc ggcccgggcc ttcctggccc
    16201 ggatccgctc ggagttcccg gacgcctccc accacgtccc cgcctacatc ctgggcgggg
    16261 gcaactcggt cacggagtac tgctccgacg acggcgagcc ttccgggacc gcgggccggc
    16321 ccctcctggc ggtcctcaag ggctccggcc tgggggacgc cgcggtcgtc gtgacccgct
    16381 acttcggggg gaccctcctg ggcaccgggg ggctcgtgaa ggcctattcc gaggcgggaa
    16441 gggccgtcct ggcgctcgcg cgtcgggctt ccctggtgga gacgggacga ttcgagctgg
    16501 acctgcccta ccgacttttc gaccgcttcc aggtcctcgc cgaagagctc ggggcggtcg
    16561 tcgcggagcg gagcttcggg gaggacgtgc gcctggccgt ggacctgccc ctgtcggccc
    16621 ggggggactt cgaggcggcc ctgacctccc tgagctccgg ggccgtcaag gtccgcctcg
    16681 aatccacccg tctcgcccgg attcccctgt agggggcccg acaggctcct aggctccgaa
    16741 cagctcggcg aactcccggg ggaaggggac gaagtccggg ggtatcccgg gcacgccccg
    16801 gaacccggcc tgccggtagg cctcgaactc cccccggacc cgggccgcgt agtcgggatc
    16861 cgaaaggatg tccgccgcgg tcagggccat ggccgaggcc gcccggatca tgccttcgtc
    16921 cccggcttcg gaggccgccg ccgcggcgaa ctccggggtg tgcaggacca ggcccgcgcc
    16981 gatgccgcag tacccgtgga tggccggagc ctcccagctg acgtccgaga agtccgtgga
    17041 agtcccgggg tagcgctgtc gctccaggtc cctccgggag tccaggggat tctccacgtc
    17101 cgcgcccagg gcccggagat gccggaagag gccggccgcc aggttcaggt tcgggatcgt
    17161 gcagcggtac tcgtccagga ccgtcacctc cccggaggcc ccggtcatcc ccgacacggc
    17221 ttccacgcag gaccggatct ccccgaacag cttgtccatg tacgcgctgt cgaaggtctt
    17281 gagggtgaaa tgggccttgg cggtctcgca tctgctgggt gggttcgaat ccatgggtt
//
LOCUS       DAOTEI010000065        17130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2268_length_17130_cov_5.405037, whole genome shotgun sequence.
ACCESSION   DAOTEI010000065 DAOTEI010000000
VERSION     DAOTEI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2268_length_17130_cov_5.405037"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="P5313_08015"
     CDS             <1..914
                     /locus_tag="P5313_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRY80346.1"
                     /translation="LTVLEQDYKILSEHLQDIVDADEQSRLEKEDEYTLLIVRLPVHD
                     SRYEPTFFTAPVGIVLFPDRVITVCWADSEVLSDMADNRVRGLSLENRNAFVLRLLGR
                     AAIVYLRHLKVLNRRTGSIERELQKSVRNTELVQLLTIEKSLVYFTTSLKSNELVLEK
                     LLKSRLIRFQEEETELLEDVEIDNRQAITMANIHSDILSGMMDAFASVISNNLNIVMK
                     RLTVISLVLMIPTFIVSAFGMNVPLPWHDSGNALWYIGGLCAVCALGGAIALTRDRRP
                     VRRSRAPVPATLVAQASGLGLPPRGAE"
     gene            980..1678
                     /locus_tag="P5313_08020"
     CDS             980..1678
                     /locus_tag="P5313_08020"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY80347.1"
                     /translation="MLEVLYRNDDLLVLDKPAGLAVQPGAGIKTSLVEAVEAQFGFRP
                     FLIHRLDRDTSGCIAVATSSRAASEYSAILSDKARCEKVYRALAAGIPEGRAGVIRDE
                     IRTPSGPRRAETRWRLLASGRGFSLLELELGTGRMHQIRIHLAGLGHPILGDDRHGDF
                     SLNKRLAKEYGLKRLLLYAARLSLRTDPPIRVEAPAPRHFRSFAEALGAEEMAAALGS
                     VDRSLDPGPDGGGA"
     gene            1675..2091
                     /locus_tag="P5313_08025"
     CDS             1675..2091
                     /locus_tag="P5313_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80348.1"
                     /translation="MTAAESPDALPPWTFRHGASWLAEGGVVLPIPGFHDEWIRSHPD
                     LVPGCSNVCDVVVRKRWVSVSVFSEGYVELLVPGRGDGESLDRCREFLARAAGAWTHA
                     LLMTMDEEGYIRLVPSDTAGPEAFLERIGTGAGFRL"
     gene            2370..3215
                     /locus_tag="P5313_08030"
     CDS             2370..3215
                     /locus_tag="P5313_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY80349.1"
                     /translation="MRKTKAIRWVRRGVQVFFFALVALISVNHTLAESGKAIPWLAGA
                     SLHSICPFGGVESIWSAITGAPLVKKVHESTFVLLWIALGLSLIAGPVVCGWVCPLGS
                     LQEWVGALGRKLFPKRYNKFVPPRLDKVLRYLRYAVLAIVVYATSRAGTLVFESWDPY
                     FALFNFWSPEITLSALAVLALVLVLALFVERPFCKYACPFGAVQGVFNLFRIFGIRRN
                     APTCINCKACDKACPMNIEVSTAGRVRDHQCITCLECTSEAACPVAATVDLAAFEKPA
                     AAAAN"
     gene            3226..4104
                     /locus_tag="P5313_08035"
     CDS             3226..4104
                     /locus_tag="P5313_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80350.1"
                     /translation="MKIKIIPLAVVTLAAFTFGIAGASALGYWITESKKEPAKVASGE
                     FAGQADPADIRGSYSFGDVEKAFGVPAAVLADAFGFTDRPAAEVKAKDLETLYAGKTG
                     ALEVGTDAVRLFTAAWIGMPYEPEADTGLPARAVELLEGRALHPATAALVRSRIVGAL
                     PAIGTLSAAPSQPASPTASAPAPSTPAAPAPAAAPVSAAPAATPSAPGSGTAPAATDT
                     HIAEAAAAREVKGSTTFGDLSTWGLSRAQIKEALGGRDPGAATEKVRDFCIAAGVEFS
                     TVKAKLQEMASALPKP"
     gene            4304..5284
                     /locus_tag="P5313_08040"
     CDS             4304..5284
                     /locus_tag="P5313_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY80351.1"
                     /translation="MKRCVVFAALLAVILCGAAAQKTVELKFGHYSVETHPAHLAAQM
                     FADAVSARTNGAVKVTIFPNSKLGSSQEMVEQAVLGAIDLVIPTEPALAKYSPKFNLV
                     GGPFAFKDYAATDRFFAGDFLKWVGPDLEKAGLQYLARWEWGFRTYTNSKKPIEKPED
                     MRGMKIRTPPDFVNQETVRALGGIAQTIAFAELPLALKQGVVDGQENPIGVIYSGKMY
                     ETQKYLSILNYTYVATHLVMNRSKFRSLTAAQQKVVLEEAKKAGEFMMKSVREQESGQ
                     IAEMKKLGMEVNYPDVAPFQAATASVWDKLKAQAGEENYKKFQELLAKSR"
     gene            5380..6681
                     /locus_tag="P5313_08045"
     CDS             5380..6681
                     /locus_tag="P5313_08045"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY80352.1"
                     /translation="MLTLAVFAVLVFLILVNLPIFVAIGSTAVLFFAGLGQGNFLAML
                     PQRMYAGTTGFTLLAIPFFILAGNLMNTGGITNRIFRFARALVGHVPGGLGQVSVISS
                     MIFSGMSGSAVADAAGLGQIQHKAMVDNGYQPVVSASIVAAASTIGPVIPPSIPFVLY
                     GALTSVSVARLFLAGFAPGVMMGLALMVVIAVMAKPRGFPRSGRASFREVAVSFRDAF
                     LPLLAPVIIIGGILSGLFTPTEASVVVCLYALFLGFLYRDLRVRDLPEILWTSIRQSV
                     GLLLIMAAANFFGWFTIFQQIPDNIISALTSLSAGGPGVMAMIVLIVLVLGCFIEGNA
                     IFLITIPIFLPVARQYGFDLVNLGVVMTLLIMIGNLTPPLGMCLFAVSSFSKIGIGDL
                     ARESWPYILGILLITLLIAYVPEIATFLPNLVMGPELGGMP"
     gene            6678..7175
                     /locus_tag="P5313_08050"
     CDS             6678..7175
                     /locus_tag="P5313_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY80353.1"
                     /translation="MKFLERLDAAVLAALKLLTIGLFSALMLILTANVFVRFVPILSL
                     HWLDEIVELLFAALVFYGSAALWILEGHFQAGEWLAKSLKSPRMRAALRLAVDLGSLL
                     FLAVFFRYSFQLVRRSMEVTAVFLIPKRVLYSCMPLSSAVMLFYSLRFVLRDFRELRT
                     PTDTR"
     gene            complement(7409..12136)
                     /locus_tag="P5313_08055"
     CDS             complement(7409..12136)
                     /locus_tag="P5313_08055"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY80354.1"
                     /translation="MPRRGPSGFAAALLLAAAAFLVPATAAAQDYYWENPRFLSDPSA
                     RYPAAFEVPGGIAVVWQESQAAGSGSGEAFLSLALLRDGKPEALNRRFAGPYPFRGDE
                     PVLLSAASSPSGELAVAVLSGDREATVLVSRDGGLSFPVTATVASDLTISAPRIFPRS
                     GGGWYLFAARGREDSLTIYYARTEDGRTWTEFRPFVNGGDNLPLSFLPSAASVQGADV
                     VVFQALSGGDRPTYQIYSKRTTDGGASWSPALQATNFGNESGSAAADTFQNQRPHLAA
                     IGGRLYMAWERTPRGGSPQAFFAELDASGAYVPGTAERISLGSGTAGEPRLIDAGGRP
                     GLIWFDNRRGRNRVYLSFREGILWRERDVSGAAQGEATFGRSVYRQGRLWVFWQSQPA
                     SGGPRISILAPDTTVRPPAAAAADFAAGRRTRRDSASVRWTAPEDSSGIAGYSYSWSR
                     DPEAVPSGELMALETVNRASFTADRDGSWWFAIRAVDYAGNWSEPARVEFVRDTTPPG
                     PPTLRPPAAGADGFLSSNTFNVLWDPPEDPDVAGYTWTLRYVGPLDRPPVRRRPAGTA
                     AVPAAESASGDSGTDTAAAAPQTGGTAQAAPYPFEPATDYERELAFRAGDLIPPPASR
                     GPGTSAGFPNVDDGYYIFSVAAIDEVGNIGPAVRTFLRADKFIPFTLVSDILQVRDDF
                     GAATLRILGRGFREDGVVSRVVLDRDGAAPYDYVFDLSAGAYSVESDRSLGGLVAQDV
                     EAGDYRVGLLHPARGWYWVRTPLSFDASGTVKFGDFSARYEPVWTFRPPARYRISFWD
                     LFAVLAAAFGAVGILATLRQAAAVIRDGSVVRLEVAALLEGGPMPTARKKTEVRRFKT
                     RGLNLGFKFTFVIGLLVLFVTAMVSVPLGVFQVRTQGQNLARGLEQKAQVLLESLAQG
                     ARTYLPAENVLELGFLPQQVQALAEARYVTITGYGTRDDRERSTNPDIVWASNDPDGI
                     SKLDGPELRPGRSALTDSISARIPEIAAAVDKRAAEEVGAIAETLARLQEEARALAIR
                     ITPENETKLSQIAATTRDLERTLNERLQVIAREIMGSEPAYDPAALDPGTTEYVFYKP
                     IVFRQGQDGIYYRGMVRLAVSTDLINQELRSATINLVRITGSIALLVLAVGVGGAALL
                     SWYIVSPIRKLVAAIERIRDTEDKEDLKGERIEEASRTDEIGSLASAVNTLTEGLVKA
                     AAASKDLTVGKETQKMFLPLHKNERGDKLSTGSEDTPDVSFFGYYEGAKGVSGDFFNF
                     MKLDDRYYAFIKCDAAGKGIPAAIISVEVATLFLNHFTGWTRDRGIELDKLCYQINDL
                     VEGRGFKGRFAALTMGVLDGKTGITQVCHAGDKTQRIYEARNRKIAVHDLPDTPPCGT
                     FANFLVEMKSPYRQVTLKLEKGDILLLYTDGIEEAKWYQRGPDFRILERTVTLKDGTE
                     QIQKLEDPFGNERAEAIVEAVLSRGRYRLEKEGNPVPDQVMTFDYSACEGTLEEMVLA
                     LISAEKLFRLYPDPRATDKDLVLVDAKIDDFLEKHFDQYRILLKDKRRDNPDKEHPEY
                     RVYAGIREDDQYDDLTLMVIKRK"
     gene            complement(12143..15280)
                     /locus_tag="P5313_08060"
     CDS             complement(12143..15280)
                     /locus_tag="P5313_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80355.1"
                     /translation="MRRKPSRIALGALLLPILLLLAAPRAAALGSRENPLAVADELIK
                     AQEYNRAIDVLRQYIQANPAGLDLAQRRLNRIAAVQSDFNKTAKELLTAFVDEPAAAE
                     KHARLIQRLRDLIPKPGETEKSFIQYAERTSIKVLWDQQRLGILQEAADQAARGLYVE
                     SARTNTRGFTLYRQQFDQDFRGKVALGVVTEGGESGKDGVDRAFETVAEAERQIGRFA
                     ELQAELASALPPLSSAFDSGDPARVDAALPAAEAALTRLAGIRAETLDTGRFLENLSL
                     LFKEKDPGLENDFFVPFAASFILGRPQAERLEGVAGSMAAQWAALFDSAAGAADAALA
                     RKAAYAQAAFSEGRFPDSDSGFRSVPPLADRAIRFQRLWSLFLPTDVTDPPSAFGRTI
                     VSVRGSDYLRLQHLRDTAQASAALAALRTELAAQEARERELTAALDAALAAAPSGPGS
                     PEAALADGLTVLRGLRTRTAELRKGISDLDAAARTRGAELARLSGAGGAPRGSQEVQT
                     ALEDRLTRSSEAAAAFELRTVALAAKAETDVQEYRLKSRTADIAAARRLAEGAVPEGA
                     PQGTAPLAYPTRSLQVIAESERLLQVLRRDVAGILSRYSAEPASFSSAPAVTAQLERA
                     RALDAAAARLSAEGQTLAAAARDRQRQAQSNRLEADRRLAEARAAYGREDFDTARERL
                     ERARERYLASLSFEDDPALRAKSDLDLQNLGTQIVQAENERVVRDTRRLLTEGKALYN
                     AGDFARAEEALLQARARWRVTHTDEPEPEVENWLRLVQTALSVKTGRDIPQTAPLYPE
                     MSQLLSLARRNYEDGRAALERRDRVSALQSFETAKQRIAQVKLVFPLNQEARVLELRI
                     NQVSDPDAFNREFARLVSQARSKIDARQDLETVYSDLLDLQTIDPKYTGLAALIERLE
                     ILIGLRLPPPDPRALAEARTLTTAAQRIWDARNVSQFSIALTQLNRALELDPNNQSAS
                     ALKDRILTYVGGTAVVVLPSAGETLYNEAVGFLQTGDFLNARIRLTRLYETYPQARKV
                     QKVSDLDSRLVARGY"
     gene            15452..16714
                     /locus_tag="P5313_08065"
     CDS             15452..16714
                     /locus_tag="P5313_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HRY80356.1"
                     /translation="MKRFRSSAASLLLLLVPALAGAQGLPSVYDSIASLFDIDLNAGL
                     TRMLITRIPMGGLAEGMGMAYTAVAADSSFLELNPSASSVQDFTELSIYHNNWIADTR
                     IEGAVYTLRFGRTGFSLGGKWLYLPFTEYNDYGDRLASGYYSEALLIGNASVHLFPGY
                     YFYGLAVGANVKLAYQSFPDFADQNGIVQAGSGFSQSALAVMADFGVLTKFNFLKYYA
                     SRKKNVSVGLALKNVGPPVQGDPLPTTATVGLAYSPLRPITISSDFSLPINLVDLSKS
                     ETPYWSLGTQFVLTDFLSMQAGLQIKGSNPRLTVGTAVDFNPLTLFVNYTLDLTTQLT
                     SLNHLSIQARFRLGDMGRAAKSAKVDELYTAGLEAYTRGDIERAIVFWTEAISLDPSF
                     DPARDALRAARASLDIREGMRNILQSTN"
     gene            16796..>17130
                     /locus_tag="P5313_08070"
     CDS             16796..>17130
                     /locus_tag="P5313_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRY80357.1"
                     /translation="MAFSIVLRLAAAAASLYMLLLMARIILTWFPSAVEGRPLHLLSS
                     VTDPYLDLFRGIPWLSTGSMDFSPLAALAVLSVLTQILSTAAAYGRISLGIVLALVLR
                     AGWSIFAFF"
BASE COUNT         2580 a         5844 c         5753 g         2953 t
ORIGIN      
        1 acctgaccgt cctggagcag gattacaaga tcctgtccga gcatctccag gacatcgtgg
       61 acgcggacga gcagtcccgg ctggaaaaag aggacgagta caccctgctg atcgtccgtc
      121 tgcccgtcca tgacagccgc tacgaaccga ccttcttcac cgcccccgtg ggcatcgtcc
      181 tattcccgga ccgggtgatc accgtctgct gggcggattc cgaggtcctg tccgacatgg
      241 cggacaaccg ggtgcggggg ctttccctgg aaaaccggaa cgccttcgtc ctgcggctcc
      301 tggggcgggc ggccatcgtc tacctccggc acctgaaggt cctgaaccgg aggacgggct
      361 ccatcgagcg ggagctccag aaatcggtgc gcaacaccga gctggtccag ctgttgacca
      421 tcgagaagtc cctggtctac ttcaccacca gcctcaagtc caacgagctg gtcctggaaa
      481 agctcctcaa gagccggctc atccggttcc aggaggagga gacggaactc ctggaggacg
      541 tggagatcga caaccggcag gccatcacca tggccaacat ccacagcgac atcctgtccg
      601 ggatgatgga cgccttcgcc tcggtcatct ccaacaacct caacattgtg atgaagcgtc
      661 tgaccgtgat ctccctggtg ctcatgatcc cgaccttcat cgtgagcgcc ttcgggatga
      721 acgtccccct gccctggcac gacagcggga acgccctctg gtacatcggg gggctctgcg
      781 cggtctgcgc cctgggaggg gccatagccc tgacccggga ccggaggccg gtacgcaggt
      841 cccgggcgcc ggtgcccgcc accctggtgg cccaggcttc cggcctgggg ctcccgcccc
      901 gcggggccga gtagggcggg gtgtccggac cgcgtcgccg gtcttgccat tccggagcac
      961 cggcactatc cttcgccgca tgctcgaggt gctgtaccgg aacgacgacc tcctcgtcct
     1021 ggacaagccc gccggcctgg ccgtccagcc cggggcgggc atcaagacca gcctggtgga
     1081 ggccgtggag gcgcagttcg ggttccggcc cttcctgatc caccggctgg accgggacac
     1141 ctcgggctgc atcgccgtgg ccacgagctc ccgggcggcc tccgagtatt ccgcgatcct
     1201 ttcggacaag gcccgctgcg agaaggtcta ccgagccctg gccgcgggga ttccggaagg
     1261 ccgggccggg gtcatccggg acgagatccg gactccctcg ggcccccggc gggccgagac
     1321 ccggtggcgc ctcctggcct cgggccgggg attctccctg ctggaactgg agctgggaac
     1381 gggccgcatg caccagatcc ggatccatct ggcgggcctc gggcatccca tcctggggga
     1441 cgaccggcac ggcgactttt ccctcaacaa gcgtctggcc aaggaatacg gcctcaaacg
     1501 cctgcttcta tacgcggccc ggctgtccct gcggaccgat cccccgatcc gggtcgaggc
     1561 cccggcgcct aggcacttcc ggtccttcgc cgaggccctg ggggccgagg agatggcggc
     1621 cgccctcggg tccgtcgacc gatccctcga tcccggcccg gacggaggcg gcgcgtgacc
     1681 gcggcggagt ccccggacgc cctccccccc tggaccttcc ggcacggtgc gagctggctg
     1741 gccgaaggcg gcgtcgtcct gcccatcccg ggcttccacg acgaatggat acggtcccac
     1801 ccggacctgg tgcccgggtg ctccaacgtc tgcgacgtgg tggtgcggaa gcggtgggtg
     1861 agcgtctcgg tcttctccga gggctacgtg gagctcctgg ttccggggcg gggcgacggg
     1921 gagagcctgg accggtgccg ggagttcctg gcccgggcgg ccggcgcctg gacccacgca
     1981 cttctcatga cgatggacga ggagggctac atacgccttg tcccctcgga tacggccggc
     2041 ccggaggcct tcctggagag gatcggcacg ggggccggat tccggctctg acggccgatc
     2101 ccccggcatc cccgagccgg cggcccggct tccgtctcgg gactcccgag gcgagggagc
     2161 gggcaggctt ttcgccgtac gtgaaatatt tgccccaaat ctggatttcc tgaccgccgt
     2221 cttgtacaat ttcctccggg gccggctcgc ggcagatcga gacaagttca gcttagacaa
     2281 tcaaataggt ctggcggata taattaaaat atcagatata ggaaaattgg catataaatt
     2341 gctatatgag gaatcaatag gaggttccca tgcgaaaaac caaggcgatc cgatgggtcc
     2401 ggcggggcgt gcaggtcttt ttcttcgccc tcgtggccct gatctcggtg aaccacaccc
     2461 tggcggagag cggcaaggcg attccctggc tggccggagc cagccttcac tccatctgcc
     2521 ccttcggcgg cgtggagtcc atctggagcg ccataaccgg ggctcccctg gtcaagaagg
     2581 tccacgagtc gaccttcgtg ctcctgtgga tagccctggg cctgtccctg atcgcgggtc
     2641 ccgtggtctg cggctgggtc tgccccctgg ggagcctcca ggaatgggtg ggcgctctgg
     2701 gccggaagct cttcccgaaa cgctacaaca aattcgtgcc cccccgtctg gacaaggtcc
     2761 tgcggtacct gcggtacgcg gtcctggcca tcgtcgtgta cgccaccagc cgggcgggca
     2821 ccctggtctt cgagtcctgg gatccctact tcgccctgtt caacttctgg tcccccgaga
     2881 tcaccctgtc cgcccttgcg gtcctggccc ttgtcctcgt cctggccctc ttcgtggagc
     2941 ggcccttctg caagtacgcc tgccccttcg gcgccgtaca gggggtcttc aacctcttcc
     3001 ggatcttcgg gatccgcaga aacgccccca cctgcatcaa ctgcaaggcc tgcgacaagg
     3061 cctgcccgat gaacatcgag gtctccacgg cgggccgggt gcgggatcat cagtgcatca
     3121 cctgcctgga gtgcaccagc gaggccgcct gcccggtggc ggccaccgtg gacctggcgg
     3181 ccttcgagaa gcccgcggcc gcggcggcga actaaggagt cggccatgaa gatcaagatc
     3241 atccccctgg cggtcgtcac cctggccgcc ttcaccttcg gcatcgccgg ggcctccgct
     3301 ctgggatact ggatcacgga atcgaagaag gagcccgcca aggtggcctc cggcgagttc
     3361 gccggacagg cggatcccgc ggacatccgg ggctcctatt ccttcgggga cgtggagaag
     3421 gctttcggag tgcccgcggc cgtcctggcg gacgccttcg gattcacgga ccggcccgcc
     3481 gcggaggtca aggccaagga tctggagacc ctctacgccg gcaagaccgg ggccctggag
     3541 gtcggcacgg acgcggtccg cttgttcacg gccgcctgga tcggcatgcc ctacgagccc
     3601 gaggcggata cggggcttcc cgcccgggcg gtggagctcc tggaaggcag ggccctgcac
     3661 cccgcgaccg cagccctggt ccgttcccgg atcgtggggg cgctgcctgc gatcgggacc
     3721 ctgagcgcgg cgccttccca gcccgcgtct ccgaccgcgt ccgcccccgc tccatcgacc
     3781 cccgccgccc ccgctcccgc ggcggcgccg gtttccgcgg ccccggccgc gacgccgtcg
     3841 gctccgggct ccgggaccgc cccggccgcg acggacaccc atatcgccga agccgcggcg
     3901 gcgcgggaag tgaagggcag caccaccttc ggggacctga gcacctgggg cctcagccgg
     3961 gcccagatca aggaagccct gggaggccgg gatcccggtg ccgccacgga aaaggtgcgg
     4021 gacttctgca tcgccgcggg ggtggagttc tccacggtga aggcgaagct tcaggagatg
     4081 gcgagcgccc tgccgaaacc gtagcccgaa acccgatact atcccttgga caatggccct
     4141 ccggttccgg aaaatcccgg aacggagggc tttttccgtg cccggcgacc cgcgacgacg
     4201 aatcagaaaa aagacttgcg taatttcaaa gaatgatcct acactatccc tgatgatgaa
     4261 atacgattcc acaatatgga acgattcatc tcaaggaggc atcatgaaac gttgtgtggt
     4321 gttcgccgcc cttctcgcgg tcatcctgtg cggtgccgcg gcccagaaga cggtggagct
     4381 gaaattcggc cactactcgg tcgagaccca cccggcccac ctggcggccc agatgttcgc
     4441 ggacgccgtg tctgcccgga cgaacggggc cgtcaaggtg acgatcttcc cgaacagcaa
     4501 gctcgggagc tcccaggaaa tggtggagca ggccgtcctg ggggccatcg acctggtcat
     4561 cccgacggaa cccgccctgg ccaagtactc gcccaagttc aacctcgtcg gcggcccctt
     4621 cgcgttcaag gactacgcgg cgacggacag gttcttcgcc ggagacttcc tcaagtgggt
     4681 gggacccgac ctggagaagg ccgggctgca gtacctcgcc cgctgggagt ggggattccg
     4741 cacctacacg aattccaaga aacccatcga gaagcccgag gacatgcggg gcatgaagat
     4801 ccgcaccccc cccgacttcg tcaaccagga gaccgtccgg gccctcggcg gcatcgccca
     4861 gaccatcgcc ttcgcggaac tccccctggc cctgaagcag ggagtcgtgg acggccagga
     4921 gaaccccatc ggggtcatct actcggggaa gatgtacgag acccagaagt acctgtcgat
     4981 cctcaactac acctatgtcg cgacccacct cgtcatgaac cggtcgaagt tccggtccct
     5041 caccgcggcc cagcagaagg tcgtgctgga ggaggccaag aaggcggggg agttcatgat
     5101 gaaatccgtc cgcgagcagg aatccggcca gatcgcggag atgaagaagc tcggcatgga
     5161 ggtgaactac ccggacgtgg ccccgttcca ggccgccacg gcttccgtct gggacaagct
     5221 gaaggcccag gccggcgagg aaaactacaa gaaattccag gaactcctgg ccaagagccg
     5281 gtgatgcccg gggtcccgga catcgatcgg ggcccgtgac cttgtacctc ccggggacgg
     5341 cctccgtgcg gagccgcccc cggaaccctg gagcgtgcca tgctaaccct cgcggtattc
     5401 gccgtgctcg tcttcctgat cctcgtgaac ctccccatct tcgtggccat cggctcgacg
     5461 gcggtcctct ttttcgcggg cctcggccag ggcaacttcc tggccatgct cccccagcgg
     5521 atgtacgccg gaaccacggg gttcaccctc ctggccatcc ccttcttcat cctggcgggg
     5581 aacctcatga acaccggggg gatcacgaac cggatcttcc gcttcgcccg ggccctggtc
     5641 gggcatgtcc ccggaggcct gggacaggtt tccgtcatct cgtccatgat cttttccggc
     5701 atgtcgggct ccgcggtggc ggacgcggcg gggctggggc agatccagca caaggccatg
     5761 gtcgacaacg gctaccagcc cgtcgtgtcg gcctccatcg tagcggcggc ctccacgatc
     5821 ggccccgtga ttcctccctc cattcccttc gtgctctacg gggccctaac ctcggtgtcc
     5881 gtcgcccgcc tcttcctggc gggattcgcg cccggcgtga tgatgggcct ggccctcatg
     5941 gtcgtcatag cggtgatggc caagccccgg ggctttccgc gatccggccg ggcttccttc
     6001 cgggaggtgg ccgtaagctt ccgggacgcc ttcctgcccc tcctggctcc ggtcatcatc
     6061 attggcggca tcctctccgg cctgttcacc cccacggaag cgtccgtggt ggtctgcctc
     6121 tacgccctgt tcctgggttt cctgtaccgg gacctccgtg tacgggacct ccccgagatt
     6181 ttgtggacct ccatccgcca gtccgtgggc ctgctcctca tcatggccgc ggccaatttc
     6241 ttcggctggt tcacgatctt ccagcagata ccggacaaca tcatctcggc cctgacctcc
     6301 ctgtccgcgg gcggcccggg ggtgatggcc atgatcgtgc ttatcgtgct ggtgctgggc
     6361 tgtttcatcg agggcaacgc gatcttcctc atcacgatac cgatcttcct gcccgtggcc
     6421 cgccagtacg gcttcgacct cgtgaacctc ggggtggtca tgaccctcct catcatgatc
     6481 gggaacctga ccccgcccct ggggatgtgc ctgttcgcgg tatccagttt cagcaagata
     6541 ggcatcgggg atctggcacg ggaatcctgg ccttacatcc tgggcatcct gctcatcacc
     6601 ctcctgatcg cctacgtgcc ggagatcgcc acgttccttc cgaacctcgt catgggtccg
     6661 gagcttggag ggatgccatg aaattcctgg aacgcctgga cgcggccgtg ctggccgccc
     6721 tgaagctcct gacgatagga ttgttctccg ccctgatgct gatcctgacc gcgaacgtct
     6781 tcgtccgctt cgtgccgatc ctttccctgc actggctgga cgagatcgtg gaactgctct
     6841 tcgccgccct ggtcttctac ggctccgcgg ccctgtggat cctggaaggc cacttccagg
     6901 ccggggagtg gctagccaaa agcctgaaga gcccccggat gagggcggcc ctccggctgg
     6961 ccgtggatct gggatccctg ctcttcctgg ccgtcttctt ccggtattcg tttcagctgg
     7021 tgcgcaggtc catggaggtc accgccgtct tcctgatccc caagcgggtc ctgtactcct
     7081 gcatgcccct gtcctccgcg gtgatgctct tctattccct gaggttcgtc ctccgggatt
     7141 ttcgggagct ccggaccccg acggatacgc gatagagggg atcgacgaag ccgcgtcatc
     7201 aaccggcgtt ccacgccggt ggacggttcg gggctgtcca tgggcgccca gcggcttcgt
     7261 cgccgtgagg accgggacgc tgcgcgaccc gccgcaggct cgtgggggtc gacgaatccg
     7321 cgtcctggga cgcgaagcat ccgtccctgg gcgtattcgc ggattcgtcg ccgtgaggac
     7381 cgggccgcaa cgcggcccgg tcctcacgct atttcctctt tatcaccatc agggtcaggt
     7441 cgtcgtactg gtcgtcctcc cggatgcccg cgtagacgcg gtactccgga tgctccttgt
     7501 cgggattgtc ccggcgcttg tccttgagga ggatccggta ctggtcgaag tgcttctcca
     7561 ggaagtcgtc gatcttggcg tccaccagga ccaggtcctt gtcggtggcc cgcggatcgg
     7621 gatacaggcg gaatagtttc tccgcggaga tgagggccag gaccatctcc tccagggtgc
     7681 cctcgcaggc ggagtagtcg aaggtcatga cctggtccgg aacggggttc ccctcctttt
     7741 cgaggcgata ccggccccgg gagaggacgg cctcgacgat ggcctcggcc cgctcatttc
     7801 cgaagggatc ctccagcttc tggatctgct ccgttccgtc cttcagggtg acggtccgtt
     7861 ccaggatccg gaaatccggg ccgcgctggt accacttggc ctcctcgatg ccgtccgtgt
     7921 agaggaggag gatatcccct ttttccagct tgagggtcac ctgcctatac gggctcttca
     7981 tctccaccag gaaattggcg aaggtcccgc acgggggtgt atccggcaga tcgtggaccg
     8041 cgattttccg gttccgggct tcgtagatgc gctgtgtctt gtcccccgcg tggcagacct
     8101 gggtgatccc ggtcttcccg tccaggacac ccatggtcaa cgcggcgaac cgtcccttga
     8161 agccccggcc ctcgacgagg tcattgatct ggtagcagag cttgtccagt tcgatgcccc
     8221 ggtcccgggt ccagcccgtg aagtggttca ggaagagggt tgccacctcc acggagatga
     8281 tggccgcggg tatgcccttt ccggcggcgt cgcacttgat gaatgcatag tatcggtcgt
     8341 cgagcttcat gaagttgaag aagtcgccgg agacgccctt ggcgccctcg tagtacccga
     8401 agaaggagac gtccggggtg tcctcgctcc ccgtcgacag cttgtccccc cgttcgttct
     8461 tgtggagggg caggaacatc ttctgggttt cctttcccac cgtgaggtcc ttgctggccg
     8521 cggcggcctt cacgagcccc tcggtcaggg tgttcaccgc ggaagccagg ctcccgatct
     8581 cgtccgtccg ggaggcctcc tcgatccgct cgcccttgag atcctccttg tcctcggtgt
     8641 cccggatgcg ctcgatggcc gccaccagct tgcggatggg gctgacgatg taccaggaca
     8701 ggagggccgc gccccccacg cccacggcca ggaccaggag ggcgatggac ccggtgatgc
     8761 gcaccaggtt gatggtggcg ctccggagtt cctggttgat caggtccgtg gagaccgcca
     8821 ggcggaccat gccccggtag tagatgccgt cctggccctg ccggaatacg atgggcttgt
     8881 agaagacgta ttccgtcgtg ccgggatcca gggccgcggg gtcgtacgcg ggctcggatc
     8941 ccatgatctc gcgggcgatg acttgcaggc gttcgttcag ggtccgctcc aggtcccggg
     9001 tggtggccgc gatctgggag agcttggtct cgttctccgg ggtgatccgt atggccaggg
     9061 cccgggcttc ttcctgcagt cgcgccaggg tctccgcgat cgccccgacc tcctcggcgg
     9121 cccgcttgtc caccgccgcg gcgatctcgg ggatgcgggc ggagatggaa tcggtgagcg
     9181 cgctgcggcc cggccgcagt tccggcccgt ccagctttga gatcccgtcc gggtcgttgg
     9241 aggcccagac gatgtcggga ttggtggagc gttcccggtc gtcccgggtg ccgtacccgg
     9301 tgatggtgac ataccgggct tccgccaggg cctggacctg ctggggcagg aagcccagct
     9361 ccaggacgtt ctccgcgggc aggtaggtcc gggccccctg ggccaggctc tccaggagca
     9421 cctgggcctt ctgctcgagt cctcgggcca ggttctggcc ctgggtcctc acctggaata
     9481 cccccagggg cacggacacc atggccgtga cgaagagcac taaaaggccg atcacgaagg
     9541 tgaacttgaa tccaaggttg aggccccggg tcttgaacct ccggacctcc gtctttttcc
     9601 gggccgtggg catgggccct ccttccagca gagccgcgac ctccaggcgc acgaccgagc
     9661 cgtcccggat gacggcggcg gcctgcctta gggtcgcaag gatgccgacg gccccgaagg
     9721 ccgcggccag gaccgcgaag aggtcccaga aggaaatccg gtaccgggcc gggggacgga
     9781 aggtccagac cggctcatac cgcgcggaga agtctccgaa cttgaccgta ccggaggcgt
     9841 cgaaggaaag aggggtgcgg acccagtacc agccccgggc cggatggagc aggcccaccc
     9901 ggtagtcccc cgcctccacg tcctgggcga ccaggcctcc cagggaccgg tcggactcca
     9961 cggagtaggc ccccgcggac aggtcgaaga catagtcgta cggcgcggcc ccgtcccggt
    10021 ccaggaccac ccgggagacg accccgtcct cccggaaccc ccggcccagg atgcgcaacg
    10081 tcgccgcgcc gaaatcgtcc cggacctgca ggatgtcgga caccagggtg aaggggatga
    10141 acttgtccgc cctcaggaag gtccggaccg cgggaccgat gttcccgacc tcgtcgatgg
    10201 ccgccacgga gaagatgtag tacccgtcgt ccacgttcgg gaaccccgcg gatgttcccg
    10261 ggccccggga ggcggggggc gggatcaggt cgcccgcccg gaaggcgagc tcccgctcgt
    10321 agtccgtcgc gggttcgaag gggtagggag cggcctgcgc cgttccgccg gtctgtgggg
    10381 ccgcggcggc cgtatccgtc ccggagtccc cggacgccga ttccgccgcg gggacggccg
    10441 cggtccccgc gggacgcctc cggaccggag ggcggtccag ggggccgacg taccggaggg
    10501 tccaggtgta gcccgccacg tccgggtcct cggggggatc ccagagcacg ttgaaggtgt
    10561 tggacgagag gaagccgtcc gccccggccg ccggcggacg cagggtcggc ggccccgggg
    10621 gcgtcgtgtc ccggacgaac tccacccggg ccggctcgga ccagttgccc gcgtagtcca
    10681 cggcccggat ggcgaaccac caggacccgt cccggtccgc cgtgaaggat gcccggttga
    10741 ccgtctccag ggccatgagc tccccggagg gcacggcctc gggatcccgg ctccaggaat
    10801 aggagtaccc cgcgatgccc gaggaatcct ccggagccgt ccatcggacc gaggccgaat
    10861 cccggcgtgt gcggcggccc gccgcgaagt ccgcggccgc cgcggcgggg ggccgtacgg
    10921 tcgtgtccgg cgcgaggatg gagatccgcg ggccgccgga ggcgggctgg gactgccaga
    10981 agacccagag ccggccctgg cggtacaccg atcggccgaa ggtcgcctcc ccctgggccg
    11041 cgccggagac gtccctctcc cgccacagga tgccttcccg gaaggacagg tagacccggt
    11101 tccgcccccg gcgattgtcg aaccagatca acccgggcct gcccccggcg tcgatcaggc
    11161 gcggttcccc ggcggtgccg gagcccaggc tgatccgctc cgccgtaccg gggacgtagg
    11221 ctcccgaggc gtccagttca gcgaagaaag cctgggggga tcctccccgg ggggtccgct
    11281 cccaggccat gtacagcctg ccgcctattg cggccaggtg ggggcgctgg ttttggaaag
    11341 tgtcggcggc cgccgatccg gattcgtttc cgaaattcgt cgcctggagt gcggggctcc
    11401 aggaggctcc cccgtcggtg gtccgcttgg aatagatctg gtaggtgggc cggtcgcccc
    11461 cggacagggc ctggaaaacg accacgtccg ccccctgcac ggaggccgcg gaggggagga
    11521 aggagagggg gaggttgtcc cccccgttga cgaagggccg gaactccgtc caggtccgtc
    11581 cgtcctccgt ccgggcgtag tagatcgtga gggaatcctc ccggcctcgg gcggcgaaga
    11641 ggtaccagcc tccgccggac cggggaaaga tccggggggc ggagatggtc aggtccgagg
    11701 ccaccgtggc cgtcacgggg aacgagaggc ccccgtcccg ggagaccagg accgtggcct
    11761 cccggtcgcc ggagagcacg gccaccgcca gttccccgga aggagaggag gccgcggaca
    11821 gcagcaccgg ctcgtccccc cggaagggat agggacccgc gaagcgccgg ttcaaggctt
    11881 cgggcttgcc gtcccggagg agggccaggg aaaggaaggc ctccccggag ccggatcccg
    11941 cggcctggga ttcctgccag accacggcga tgcccccggg gacttcgaac gccgcgggat
    12001 accgggccga cgggtccgac aggaaccggg ggttctccca gtaatagtcc tgggcagccg
    12061 ccgtggccgg aaccaggaag gcggcggccg ccaggagcag ggcggccgcg aatccggaag
    12121 gaccacgtct cggcatggga cttcagtatc ccctcgcgac caggcgcgag tccaggtccg
    12181 aaaccttctg taccttgcgg gcctggggat aggtctcgta gagccgggtc aggcggatcc
    12241 gggcgttcag gaaatcgccg gtctgcagga aacccacggc ttcgttgtac agagtctcgc
    12301 ccgcgctggg caggaccacc accgcggtcc ccccgacgta ggtcaggatg cggtccttga
    12361 gggcactggc ggactggttg ttgggatcca gttccagggc ccggttgagc tgggtcaggg
    12421 cgatgctgaa ctgggatacg ttccgggcgt cccagatcct ctgggcggct gtggtcaggg
    12481 tgcgggcttc cgcaagggcc cgggggtccg ggggcggtag acgcaggccg atcaggatct
    12541 ccaggcgctc gatgagggcc gcaaggccgg tgtatttcgg atcgatcgtc tggagatcca
    12601 gaaggtcgct gtagacggtc tcgaggtcct gtcgggcgtc gatcttggac cgggcctggg
    12661 ataccagccg cgcgaattcc cggttgaagg cgtccgggtc ggacacctgg ttgatgcgga
    12721 gttccaggac ccgggcttcc tggttcaggg ggaagacgag cttgacctgg gcgatccgct
    12781 gcttggcggt ttcgaaggac tggagggcgc tgaccctgtc ccgacgctcc agggccgccc
    12841 gaccgtcctc gtaattcctg cgggccaggc tcagaagctg gctcatctcg gggtagagag
    12901 gcgcggtctg ggggatgtcc cggccggtct tgacggagag ggcggtctgc acgaggcgca
    12961 gccagttctc cacctccggt tccggctcgt ccgtgtgggt caccctccag cgggcccggg
    13021 cctggagcag ggcctcctcc gcccgggcga agtccccggc gttgtagaga gccttgccct
    13081 cggtgaggag gcggcgggtg tcccggacta cccgttcatt ttccgcctgc acgatctggg
    13141 tccccaggtt ttggaggtcc aggtcgcttt tcgcccgcag ggcggggtcg tcctcgaagg
    13201 acagggaggc caggtaccgt tcccgagccc gctccagtcg ttcccgggcg gtgtcgaagt
    13261 cctcccggcc gtaggccgcc cgggcttccg ccaggcggcg gtccgcctcc aggcggttgc
    13321 tctgggcctg gcgctggcgg tcgcgggccg ccgcggccag ggtctggccc tccgcggaga
    13381 gccgggccgc ggcggcgtcc agggcccggg cccgctcgag ctgggccgtg accgcggggg
    13441 cggaggagaa ggaggcgggc tccgccgagt accgggacag gatgcccgcg acgtccctcc
    13501 gcaggacctg caggaggcgc tcggattccg cgatcacctg gaggctccgt gtggggtagg
    13561 ccaggggggc ggtcccctgc ggggcgccct ccgggaccgc cccttccgcg aggcgccgcg
    13621 cggcggcgat gtccgcggtc cgggacttca ggcggtattc ctggacgtcc gtctcggcct
    13681 tggccgccag ggccacggtc cggagctcga aggccgcggc ggcttccgag gaccgggtca
    13741 gccggtcctc gagggcggtc tggacttcct gggaaccccg gggggctccc ccggcaccgg
    13801 aaagccgggc cagctcggcg ccccgggtcc gggccgcggc gtccaggtcg gagatgccct
    13861 tccggagctc cgcagtccgg gtccgcaagc cccgaaggac cgtgagcccg tccgccaggg
    13921 ccgcctccgg ggatcccggc ccggacgggg ccgcggcgag ggcggcgtcc agggcggccg
    13981 tgagttcccg ctcccgggct tcctgggcgg ccagctcggt ccgcagggcc gccagggccg
    14041 cggacgcctg ggcggtgtcc cggagatgct ggagccggag gtagtcggag ccccggacgg
    14101 acacgatggt ccggccgaag gcgctggggg ggtccgtgac gtccgtgggg aggaagaggc
    14161 tccacaggcg ttggaaccgg atggcccgat ccgccagggg gggtacggag cggaagcccg
    14221 agtcggaatc ggggaaccgt ccctcggaga aggcggcctg ggcgtacgcg gccttccggg
    14281 ccagggcggc gtccgccgcg ccggctgcgg agtcgaagag ggcggcccac tgggcggcca
    14341 tggagcctgc gacgccttcc aggcgttccg cctggggccg tcccaggatg aagctggccg
    14401 cgaagggcac gaagaagtcg ttctccaggc ccgggtcctt ctccttgaac aggaggctca
    14461 ggttttccag gaaccgtccg gtgtccaggg tctcggcccg tatccccgcc aagcgggtca
    14521 gggccgcctc cgcggcgggc agggctgcgt cgacgcgggc gggatccccg gaatcgaagg
    14581 cgctactcag gggcggcagg gccgaggcga gctccgcctg cagctccgcg aagcgtccta
    14641 tctgccgctc cgcctccgcc acggtctcga aggcccggtc caccccgtcc ttccccgact
    14701 cccctccctc cgtgaccacc ccgagagcca ctttcccccg gaaatcctgg tcgaactgtt
    14761 gccggtagag ggtgaagccc cgggtgttgg tccgggcgga ctccacgtag agcccccggg
    14821 ccgcctgatc cgcggcttcc tgaaggattc ccagccgttg ctggtcccag agcaccttga
    14881 tggaggtccg ttccgcgtac tggatgaagc tcttttccgt ctctcccggc ttgggtatga
    14941 ggtcccggag gcgctggatg agccgggcgt gtttctccgc ggcggccggc tcgtccacga
    15001 aggccgtgag cagttccttg gcggtcttgt tgaagtccga ctggaccgcc gcgatccggt
    15061 tgagccgcct ctgggccagg tccaagcccg cggggttggc ctggatgtac tgcctcagga
    15121 cgtcgatggc gcgattgtac tcctgggcct tgatgagttc atccgccacg gccaggggat
    15181 tttcccggga gcccagggcc gccgcccggg gtgccgcaag gaggagtaga atagggagga
    15241 gcagggctcc cagggcgatg cgggacggtt tgcgcctcat cggacgcggg ggatcctcct
    15301 tccgtacgga caatatacct cggccccggc ggttccgcgt gagtcgaggg cggtcgggcc
    15361 cctttcattc tcggcatgaa gggacggtcg atgtaggcgc aagggagcgc gtggaaatgc
    15421 cgatacgact atagtatact tgatggtaca gatgaagcgt ttccggtcct cggcagcctc
    15481 ccttctcctc ctcctggttc ccgccctcgc gggggcccag ggacttccgt ccgtctacga
    15541 ttctatcgcc tccctcttcg acatcgacct gaacgcgggg ctcacccgga tgttgatcac
    15601 ccgcatcccc atgggggggc tggcggaggg catgggcatg gcctacaccg ccgtggccgc
    15661 ggactcctcc ttcctggagc tgaacccctc ggccagctcg gtccaggact tcacggagct
    15721 ttccatctac cacaacaact ggatcgcgga tacccggatc gagggggccg tgtacaccct
    15781 ccgcttcggc cgcaccggct tctccctggg cggcaagtgg ctctacctac ccttcacgga
    15841 gtacaacgac tacggggacc gcctggcctc ggggtactac tccgaagccc tcctgatcgg
    15901 caacgcctcg gtgcacctct tcccgggtta ctacttctac gggctggcgg tcggcgccaa
    15961 cgtcaagctg gcctaccagt ccttcccgga cttcgccgac cagaacggga tcgtccaggc
    16021 cggctcgggg ttctcccagt ccgccttggc ggtcatggcg gatttcgggg ttctgacgaa
    16081 attcaacttc ctcaagtact acgcctcccg gaagaagaac gtgtccgtgg gactggcgct
    16141 gaagaacgtc ggcccccccg tccaggggga tcctctgccc acgacggcca cggtgggcct
    16201 cgcgtactcc cccctgcggc ccatcacgat ctcttcggat ttttcgcttc ccattaatct
    16261 cgtggacctt tcgaagagcg agacccccta ctggtccctg ggaacccagt tcgtcctgac
    16321 cgacttcctc tccatgcagg cgggcctgca gatcaaggga tccaatccac ggctgaccgt
    16381 ggggaccgcg gtggatttca atcccctgac cctcttcgtg aactacaccc tggacctgac
    16441 cacccagctg acctccctga accacctttc catccaggcc cggttccggc tgggggacat
    16501 gggccgggcg gccaagtccg ccaaggtgga cgagctctat acggcggggc tggaagccta
    16561 cacccggggc gatatcgagc gggcgatcgt cttctggacc gaggccatct ccctggatcc
    16621 ctccttcgac cccgcccggg acgccctacg ggccgcccgg gcctccctgg acatccggga
    16681 gggtatgagg aacatcctcc agagcaccaa ctgagatccc gccgggtttt catggtataa
    16741 aggacgtgtc gcccgtcggg gcggagaagt tcccaaccgg aaagggtaga gccctatggc
    16801 gttttccatc gtccttcgcc tcgcggccgc ggccgcgtcc ctgtacatgc tcctcctcat
    16861 ggcccgcatc atcctgacct ggttcccctc ggccgtggag ggccggcccc tgcacctact
    16921 gtcctccgtc acggacccct acctggatct cttccggggc atcccctggc ttagtacggg
    16981 gagcatggac ttctcccccc tggccgccct ggcggtgctc tcggtgctga cccagatcct
    17041 ttccaccgcg gcggcctacg gccggatcag cctggggatc gtcctggccc tggtcctgag
    17101 ggcggggtgg tcgatcttcg ccttcttctt
//
LOCUS       DAOTEI010000066        17033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2286_length_17033_cov_4.582931, whole genome shotgun sequence.
ACCESSION   DAOTEI010000066 DAOTEI010000000
VERSION     DAOTEI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 17033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17033
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_17033_cov_4.582931"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(438..2441)
                     /gene="priA"
                     /locus_tag="P5313_08075"
     CDS             complement(438..2441)
                     /gene="priA"
                     /locus_tag="P5313_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRY80358.1"
                     /translation="MNPETPVPGIPAAESSPFVEVVFPIPLDRAFTYRRLPEPPARVG
                     CRVEALLGTRKAVGWVVGETPSLPEGLGESAVKPVLRVLDSEPLFGPRTPELASWLAG
                     MYLCSRGEALAAMLPSGRREPSEASPPFEDYPPMGEPLALSAEQEAGLARILADRAGR
                     KYLYGTTGSGKTEVFLQAAEATLKEGRGVLYLVPEIALTKQVVDAVSRRFGDRCAVLH
                     SGLSPSRRLSEWRRILSGRADVVVGARSAVFAPVANLGLVVIDEEHEGSYKSGSSPRY
                     HARQVAMKRCADEGARLVLGSATPSAEAWSLMAEGTLERLDLTRRLAGGAPPVVEVVD
                     MRGEEGALSRRLAAELARVKAEGGQSILFLNRRGFSYFFRCRSCGAEIRCRHCSVGLT
                     YHKDRNRLLCHYCGYSEAPPRACLECGSLDVGWAGFGTERVEEELAARFPGLSSARLD
                     ADTAARKGELDRILDSFRDGRIDVLLGTQMVAKGLNFPGVKLVGIVLADTSLNLPDFR
                     AAERTFSLVVQVAGRAGRFSPDGRVILQTFRPESPTMRYAAAQDVEGFYAAELAARRE
                     LGFPPFSRIIRVVFRSRKADRAWEGARAFAEAAARSLPADAEILGPAECPISSMAGTF
                     RLHVVLRAPTMGGLHRSVSRLVREFEPPPDCRLETDVDPVSLL"
     gene            complement(2438..3172)
                     /locus_tag="P5313_08080"
     CDS             complement(2438..3172)
                     /locus_tag="P5313_08080"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY80359.1"
                     /translation="MDPTDRAELNTRLELLSDWLSLGCRRQRPEPVFRVPPPPETGSG
                     KRRGPEWLDDPFYPETLENIQNEVLRCAKCRLALNRRCAAFGEGVLTPEVLVVGEGPG
                     EEEDKQGRPFVGPAGKLLDKMLAAVDLDRGRNCYIANVVKCRPPMNRDPAPDEREACL
                     PYLERQIRFLRPKAILALGRTAAQTLLKSERGVAALRGAFHDRRGVPVLVTYHPSAIL
                     RDETLKRPAWEDLKRLRAFLDGGTAP"
     gene            complement(3206..4417)
                     /locus_tag="P5313_08085"
     CDS             complement(3206..4417)
                     /locus_tag="P5313_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80360.1"
                     /translation="MSPARPRGAAVSPPARRASSREPKLAVEYVRFVLFDVGRAIDLK
                     KAASVLPSRTDSKSVKRRNTPASLTLPKTLRVSLEKDAFGSPSPAAYEGAEATAKLYD
                     EGVISLSVRIRLTAGLSELHALERRTFLLGGRDCTVDQFLEERFRDLHERLGPAISRD
                     DGPDPVPERETYTAYCVCDWGRGTPDAFVAKHGRALASLLEGEPEPQDLHDLQVKKSL
                     ANPFSYGAEDLAIFDLDHCVILDRSRDWEDILLIAEHANYQLLELRVLDRLLDHWLED
                     AEKDLRAFYSQSRGRRGRKARIRALPAKFARLQGLRLEALFILENLENSAKIIGDYFL
                     GQIYVHLGRVFSVEGWTRSVERRLGALQNVYEIVKSDKSERTMLVLEIVFIIVCIAFP
                     ILQILQVMLSP"
     gene            complement(4414..5421)
                     /locus_tag="P5313_08090"
     CDS             complement(4414..5421)
                     /locus_tag="P5313_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRY80361.1"
                     /translation="MKLPIDIKRLEELDPVAHKFTLLALVGIIVLMTVSGLIAFFLAL
                     QGAEQTMVPEVTDLELTTALIKLQEKELYPRIALRFSSNPTDRGRILEQKPPAGTIVK
                     AGRRIHLTVSRGPAVNQVENYVGQTLDEVKIHLQTLFSSTRPLLAIREPPVYIFDRAP
                     AGTILEQKPVPGTEIAGLTELVFVVSRGPELARVRVPDLMGLSIPEAIRKIEESEVAF
                     AFSMRPPEGRERPGLVVSQMPAPGSLVAPDAPVSVLVSRPAPEKGMVAGLLEETLSVF
                     PYLLKVTLTAQYPTGEKAVLLTAQHPGGPFSAPYLVPNNTVLILSAANRELLRREVRS
                     E"
     gene            complement(5474..6436)
                     /gene="fmt"
                     /locus_tag="P5313_08095"
     CDS             complement(5474..6436)
                     /gene="fmt"
                     /locus_tag="P5313_08095"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRY80362.1"
                     /translation="MRVLFAGSPEIAVPALAAVVRDHDVVGVLTNPEAAKGRGLRAVR
                     TPVAEAAADLIPETPILAFERLGAEAREAAAALKPEILACFAYGRIFGPKFLALFPRG
                     GVNVHPSLLPRYRGCAPIPWAILNRESETGVTVQRLALEMDRGAILAREVVPLSGRET
                     TETLSALMAERGADLLASVLQRIERGEATEEEQDEGQATYCSLLRKEDGRVDWALPAP
                     DIDARIRAFHPWPGAYAELRGQRVNLLESEPFPEGALDAGGNGSAGRRDPGTVVALDK
                     SRGIMVQTGNGLLALRRLQLQTKKPLPFREFANGVRDLVGARFH"
     gene            complement(6444..6926)
                     /gene="def"
                     /locus_tag="P5313_08100"
     CDS             complement(6444..6926)
                     /gene="def"
                     /locus_tag="P5313_08100"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRY80363.1"
                     /translation="MEIVTLGREVLTRTAEPVKDIDDKIRSLAREMLETMRVGKGIGL
                     AAPQVDLGLRLFVTEVDERPRIFINPEIVLTSQELADLEEGCLSIPGVYAKLKRPEAV
                     RIQAWNERGRPFNLDAEGLEARVILHELDHLNGVLFVDRLSEPARKRVLAQYERRIRM
                     "
     gene            complement(7040..7498)
                     /locus_tag="P5313_08105"
     CDS             complement(7040..7498)
                     /locus_tag="P5313_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRY80364.1"
                     /translation="MRNPKSILAVLAAGLLALSSCAAQKGNPPLRTERLTVGPAQVTA
                     EIARTPGERELGLMFRKALPDGAGMLFVFDTDERLSFWMKNTLVPLSIAYISSDGTIR
                     EILDMEPRSLAPVPSQYSVRYALEVPQGWFSRAGVRVGDRVILPDSVRSR"
     gene            complement(7625..7822)
                     /gene="rpsU"
                     /locus_tag="P5313_08110"
     CDS             complement(7625..7822)
                     /gene="rpsU"
                     /locus_tag="P5313_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRY80365.1"
                     /translation="MIVDDSEPLEKAIKRFKRMVEKEGIIREWKKREFFEKPSLIKNR
                     KQKALQRKLMKKTRKSRDGKY"
     gene            complement(7890..8924)
                     /locus_tag="P5313_08115"
     CDS             complement(7890..8924)
                     /locus_tag="P5313_08115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY80366.1"
                     /translation="MRRFLSALLSLALLCASAAQDAGANPPAPAPDAVPPAIPQAAQP
                     VSPQAAQPVSPQEAPAPAAIPLPALPAEIRPGDVLLVRLPRPESGTPAGEAVLKSPAG
                     TVLQRAAGFRLGPEPGRTAFVFGLPTDAPAGIYTVEIPGGPGILPALSRHSVTVRPRD
                     FLAEDIPLSTNLTTLRVEPSPRKDEEARVYADILGRVDPDGIWLDGPFLRPVASDRRT
                     SRFGDRRRYLYATGGTASAVHAGIDFAAPTGTPVVAAGRGRVVLAADREVTGKTVVLE
                     HLPGVYTVYMHLDSLSVAPGAVLERGARLGTVGSTGLATGPHLHWELRVRGVACDPET
                     PAFAVFPAGP"
     gene            9015..9566
                     /locus_tag="P5313_08120"
     CDS             9015..9566
                     /locus_tag="P5313_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80367.1"
                     /translation="MELPFTLTETRDGRLRYGIALGYRVLAALIFGILSWTLYAQAEA
                     PSVVAWLLLALSAFGVLYEERWIFGPAGAEHAVGILFAARTLRLDREALEALRLVPHV
                     HGTVPGSEDEGRENARALSGERDADPDGRKKSLWKRPYLILVLAARDGSEYAVDKVPA
                     RNRDRILGYARRIAERTGLELRS"
     gene            complement(9758..10762)
                     /locus_tag="P5313_08125"
     CDS             complement(9758..10762)
                     /locus_tag="P5313_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY80368.1"
                     /translation="MNDNNLLEVRGLKMHFPTHQGFLWNRKKGFIYAVDGVDFSIRKG
                     ETLGLVGESGCGKSTTLRAIAQLYKPTAGEVVLKGQELTKASKQDLLAARKNMQVVFQ
                     DPYASLNPRMTTGSIIAEPLRIFKQRGLLDISDKEMDARAEQLMERVGLSRFFKNRYP
                     HEFSGGQRQRIGIARALALKPELVLCDEPVSALDVSIQAQILNLFKDLQEEFGLTYLF
                     IAHDLSVIQYISDRVAVMYLGVIVETAKSSELYKSPMHPYTQALLSAAPIPDPKIERK
                     RQRIILTGDVPSPDKARTGCYFYDRCSKRMDKCRDNLPRLKDSGPDHQVACFLYHEPD
                     "
     gene            complement(10764..11747)
                     /locus_tag="P5313_08130"
     CDS             complement(10764..11747)
                     /locus_tag="P5313_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80369.1"
                     /translation="MTDDVILQVKDLKTYFKLDEGLLKAVDGVSFDLRKGETLGIVGE
                     SGSGKSVTNLAVMKLIPVPPGRIAGGEVLFQGKDLLKMPDDEIRTIRGNKISMIFQDP
                     MTSLNPFLRISTQMIETIVLHQGMNKKDAKDKSIEMLKLAGIPSPEKRIDSYPHQFSG
                     GMRQRVMIAMALSCNPEVLIADEPTTALDVTIQAQILELIKDLTARLGTAVILITHSL
                     GVVAGMCDNINVMYAGRIVERGTADEIFGDPKHPYTQGLIKSVPRLDKATQQRLFSIK
                     GQPPNVINLPDCCPFYPRCDRALDICKIKYPATAELSKGRNVACWLYTEGK"
     gene            complement(11768..13018)
                     /locus_tag="P5313_08135"
     CDS             complement(11768..13018)
                     /locus_tag="P5313_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY80370.1"
                     /translation="MANIDTTQDAVNEFNQYVKPVSLWADAWKRLRRNKMAVLALWVV
                     GFYFLLALSAPLLQSAGILHSYTEQIISHQNLRPSFRPAGELVIESLELRIAQLEENL
                     ASREEDSNSTYNMATGFDFAPAAEIEFDDGEVDEGEAALEEVTEEADDTYYGPTYSDS
                     VVDELERSKAEVEALRGSIHTDPQHSKVYILGTDDLGRDMLARTIYGGRISIMIGIVG
                     TLTAVLIGILIGSISGYVGGAIDNFLMRFVDIMYSLPYMLVVIIIMSMVGDKGRGSIV
                     ILFVAIALISWLTISRVVRGQIISLKNSEFVEAARSMGAGTPRIIFRHLLPNTLGVII
                     VFSTLLMPSFIMQESFLSFLGLGVSAPQASWGTLVSEGVRGMELRAWQLFVPAISMTV
                     FLFCMNFLGDGLRDALDPQSKNRG"
     gene            complement(13027..13953)
                     /locus_tag="P5313_08140"
     CDS             complement(13027..13953)
                     /locus_tag="P5313_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY80371.1"
                     /translation="MARFFIRRLLSLVPTMFIIVTLSFFLIRLAPGGPFARERDLPEA
                     ILQNILKRYHMDEPLPKQYLRYLGDVIRWDFGPSLRYRDRSVNEMIDTTLPVSTALGL
                     TALSLAVILGVNVGIVSALKQNKWPDYTAMSVAVVGISVPLFVMGPILQMVFAIWLKW
                     LPPGQWLSDHGLKAIILPAVTLSFPYFAYIARLSRASVLEVLRSDYIRTARAKGLKES
                     VVIVKHVLKGALLPVVTYLGPALSGIVTGSVVVEAVFVVPGLGRPFVQAAINRDYTMI
                     MADVIVYSLLLITANLVVDLVYGLLDPRISYK"
     gene            complement(14117..15673)
                     /locus_tag="P5313_08145"
     CDS             complement(14117..15673)
                     /locus_tag="P5313_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY80372.1"
                     /translation="MKRVLLVMLALLAVAGIVGAQSKGQEIIMNNGAEPQTLDPHHIE
                     GVPEHRIYMALFEGLTTHDPKTSRAVPGVAEKWTFSKDLKTVTFKLRKAVWSDGTPIT
                     AKDFVESWIRKLDPKEAAQYADLLYVVEGAEAYNTGKAGPEALKIRAVDDSTFEVKLV
                     GPTPHFPDMVAHYAFAAVPMHAVKKHGLDWVKPGNFVGNGPFLLQEWKPQERLVFVPN
                     PKYWDAKTVKLQKITLLPIDDLSTSYQMCKAGKVDWLDAVPLELMDEIKLRKDYQNAP
                     YYGTYYYIYNVTRKPLDDPKVRKALSMAFDRQALVDKVTKGGQIPAFTFVPASAGFKP
                     PAGLGYNIEQAKKLLAEAGYPDGKGFPTFQILYNTSSGHQRIAAFIQEEWKRNLGITV
                     TLINQEWSTYLDTRSQSHDFDIARAGWIGDYLDPSTFSDMWIIGGTQNDGLYNSKEYT
                     DLIFKARTQSGATRMKTLMQAEDVLIMKDHAIMPIYFYVTNNLIDLSKWGGWYLNPLG
                     SHPWKYIYRK"
     gene            complement(15784..16872)
                     /locus_tag="P5313_08150"
     CDS             complement(15784..16872)
                     /locus_tag="P5313_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRY80373.1"
                     /translation="MYSYVIILAGGSGTRLWPASTSARPKQFLALGDGSSFFRRALDR
                     ALALAPARGILIVTGASMAEAVAEECRALPPGAPGRGSVRILPEPAGRNTAPAVAWAL
                     AAAEAWGGRPEDTFLLMTSDHLIEPASAFAADAAKAEALAAAGNLVCFGIPPRYPATG
                     YGYVEAAGPLGPGFRAAGFREKPDAATAARFLEAGKYYWNSGMYAFRSDFLRRELAAH
                     APEVARAFDSLIDRPREAERGGVRIVEAWEGLEEAYERAPSVSLDYAVSEKCASVALV
                     PASFTWEDVGSWDEMARLFPGGSGNAVAVESEACYVDSDIPVALCGVSDLVVVIRNGA
                     ALVLKKGSSQLVREAVERLKSQGRKDLL"
BASE COUNT         2720 a         5476 c         5966 g         2871 t
ORIGIN      
        1 cccctccatg tcccgctggt ggttccgcta cggccaggcg gtggaggccc tctcggacta
       61 tcgcagggcc gcggacctct acgcccgggc cctcaagctc tccccggaga tgaaggaagc
      121 ctcggacgcc ctggcccggg tccgttcccg gatataaacg aagcctgcgc accgtgatag
      181 ttgccgaaag ctttccggaa tgaaccacgg agtgcacgga gatcacggag aaagacagag
      241 atcagtcaaa gaaatttctc tggattaaca gtatgaaggc cggatgaaca ggatacgggt
      301 ggaagtgaat tctccaagat cttgatcccg tccatcgttc ttccatcttg tttatcctgt
      361 ctctcttcgt gatttagccg cgctatcttt tcccctccgt gtccttcgtg ctctccgtgg
      421 tgaatcctga aatcgtttta cagtaaactg accggatcca cgtccgtctc caggcggcag
      481 tccgggggcg gctcgaactc ccgcaccaac cgggagaccg atctgtggag gccccccatg
      541 gtgggcgcac ggaggaccac gtggagccgg aaggtcccgg ccatggagga gatggggcat
      601 tccgcgggac ccaggatctc cgcgtccgcg ggaaggctcc gggcggccgc ctcggcgaaa
      661 gcccgggccc cctcccaggc gcggtcggcc ttccgggacc ggaacaccac ccggatgatc
      721 cgggagaagg gagggaagcc cagctcccgg cgggccgcca gttcggcggc gtagaagccc
      781 tccacgtcct gggccgccgc gtagcgcatc gtgggcgact cggggcggaa ggtctgcagg
      841 atcacccggc cgtccgggga gaaccggccc gcccggcccg ccacctgtac gaccagggag
      901 aaggtcctct ccgcggcccg gaagtccggg aggttcaggg aggtgtccgc caggacgatg
      961 cccacgagct tcacccccgg gaaattgagc cccttggcta ccatctgggt tcccaaaagg
     1021 acgtcgatcc tcccgtcccg gaaggagtcc aggatccggt ccagctcccc cttccgggcc
     1081 gcggtgtccg cgtccagccg ggccgaggag aggcccggga acctggcggc cagttcctcc
     1141 tccacccgct cggtgccgaa gcccgcccag cccacgtcca gggacccgca ttccaggcag
     1201 gcccggggag gcgcctcgga atagccgcag tagtggcaca agaggcggtt ccggtccttg
     1261 tggtaggtga gccccacgga gcagtggcgg cagcggatct cggccccgca ggaccggcag
     1321 cggaagaagt acgaaaagcc ccggcggttc aggaagagga tgctctggcc gccctcggcc
     1381 ttgacccggg cgagctccgc ggccaggcgg cgggacaggg ccccctcctc gccccgcatg
     1441 tccacgacct ccacgaccgg aggcgccccg cctgccaggc gccgggtgag gtccagccgc
     1501 tccagggtcc cctcggccat gaggctccag gcctccgcgg agggagtggc ggaacccagg
     1561 accagccggg ccccctcgtc ggcgcaacgc ttcatggcca cctgccgggc gtggtagcgg
     1621 ggggaggatc cggacttgta ggagccctcg tgctcctcgt cgatcaccac caggcccagg
     1681 ttggcgacgg gggcaaagac cgcgctgcgg gcgcccacca ccacgtcggc ccgcccggaa
     1741 aggatccgcc tccattccga aaggcgccgg gagggggaga gccccgagtg gaggaccgcg
     1801 cagcggtccc cgaaacggcg ggacacggcg tccacgacct gcttggtcag tgctatctcc
     1861 gggaccaggt agaggacccc ccggccttcc ttgagggtgg cctccgcggc ctgcaggaag
     1921 acctcggtct tcccggaccc ggtggtgccg tacaggtatt tccgtcccgc ccggtccgcc
     1981 aggatccggg ccagccccgc ctcctgctcc gcggagaggg ccaggggttc gcccatcggg
     2041 ggatagtcct cgaagggcgg ggatgcctcc gagggctccc ggcggcccga ggggagcatg
     2101 gccgccaggg cctcgccccg ggaacagagg tacatccccg ccagccagga ggccagctcg
     2161 ggggtccggg gcccgaagag gggctcggaa tccaggaccc gcagcacggg tttgacggcg
     2221 gattccccca ggccctcggg gagcgaggga gtctctccga ccacccagcc cacggctttc
     2281 cgggtcccca ggagggcctc caccctgcag cccacccggg cgggcggttc cgggagccgg
     2341 cggtacgtga aggcccggtc cagggggatg gggaagacta cctcgacgaa gggcgagctc
     2401 tcagcggcgg gaatcccggg aaccggggtc tccgggttca cggcgccgtt ccgccgtcca
     2461 ggaaggcccg gagccgtttg aggtcctccc aggcggggcg cttcagggtc tcgtcccgga
     2521 gtatcgcgct ggggtggtag gtcaccagga cggggactcc ccggcgatcg tggaaggcgc
     2581 cccggagggc ggccacgccg cgctcgctct tgagcagggt ctgggccgcg gtcctgccca
     2641 gggccaggat cgccttcggc cgcaggaagc ggatctggcg ctccaggtag ggcaggcagg
     2701 cttcccgctc gtccggggcg ggatcccggt tcatgggggg gcggcacttc acgacgttgg
     2761 cgatgtagca gttccgcccc cggtccaggt ccaccgcggc cagcatcttg tccaggagct
     2821 tcccggcggg tcccacgaag ggccggccct gcttgtcctc ctcctccccg gggccctcac
     2881 ccacgaccag gacctcgggg gtcaggaccc cctccccgaa ggcggcgcac ctgcggttca
     2941 gggccagccg gcacttggcg cagcggagca cctcgttctg gatgttctcc agggtttccg
     3001 ggtagaaggg atcgtccagc cactccggtc cccggcgttt gccggagccg gtctcggggg
     3061 gcggggggac ccggaatacc ggttcgggcc gctgtctgcg gcatcccagg gagagccagt
     3121 ccgagaggag ttccagacgg gtgttgagtt ccgcgcggtc ggtcgggtcc atggggggga
     3181 ttgtatcgtg cggggccggg aaccgctagg gcgagagcat cacctgcagg atctgcagga
     3241 tgggaaaggc gatgcagacg atgatgaaga cgatctccag gacgagcatg gtacgctcgc
     3301 tcttgtcgct cttgacgatc tcgtagacgt tctggagggc tcccaggcgt cgctccacgc
     3361 tccgggtcca gccttccacg ctgaagacgc gccccaggtg gacgtagatc tggcccagga
     3421 agtagtcccc gatgatcttg gcggagttct ccaggttctc caggatgaag agggcctcca
     3481 gccggagccc ctggaggcgg gcgaacttgg cgggcagggc ccggatgcgg gccttgcggc
     3541 cccgccggcc ccggctctgg gagtagaagg cgcgaaggtc cttctccgcg tcctccagcc
     3601 agtggtccag gaggcggtcc aggacccgga gctccaggag ctggtagttg gcgtgctccg
     3661 cgatcaggag gatgtcctcc cagtcccggg accgatccag gatgacgcag tggtccaggt
     3721 cgaagatggc caggtcctct gcgccgtagg agaagggatt ggccagggac ttcttgacct
     3781 ggaggtcgtg gaggtcctgg ggctccggct cgccctccag gagggacgcc agggcgcggc
     3841 cgtgcttggc cacgaaggcg tccggggttc cccgccccca gtcgcacacg cagtaggccg
     3901 tgtaggtttc ccgctcgggc accgggtccg ggccgtcgtc ccgggagatc gccgggccca
     3961 ggcgctcgtg caggtcccgg aaccgttcct ccaggaactg gtccaccgtg cagtcccggc
     4021 cgcccaggag gaaggtcctg cgctccaggg cgtggagctc cgagagcccc gcggtcaggc
     4081 ggatccggac cgagagggag atgaccccct cgtcgtagag cttggccgtg gcctccgccc
     4141 cctcgtaggc cgccggggac ggggacccga aggcgtcctt ctccagggag acccggaggg
     4201 tcttgggcag ggtgagggag gccggggtgt tccggcgctt gacggacttg gagtccgtgc
     4261 ggctcggcag gaccgaagcc gccttcttga ggtcgatggc ccggcccacg tcgaacagga
     4321 cgaagcgtac gtattccacc gcgagcttgg gctcccggga ggatgcccgg cgtgcgggag
     4381 gggataccgc ggcccccctc ggccgggccg ggctcattcg gaccgcacct cccggcgcag
     4441 gagctcccgg ttggcggcgc tcaggatgag gaccgtgttg ttcgggacca ggtagggagc
     4501 cgagaaggga ccgccggggt gctgggccgt gaggaggacc gccttctccc ccgtggggta
     4561 ttgggccgtg agggtgacct tgagcaggta ggggaagacc gagagggttt cctccagcag
     4621 gccggcgacc atgcccttct ccggggcggg ccgggacacc aggacgctga ccggggcgtc
     4681 cggggccacc agggagcccg gggcgggcat ctgggagacc accaggcccg gacgctcccg
     4741 gccctcggga ggccgcatgg agaaggcgaa ggcgacctcc gactcctcga tcttccggat
     4801 cgcctccggg atggacaggc ccatgaggtc cggcacccgg acccgggcca gctccggccc
     4861 ccggctgacc acgaacacca gctccgtcag gcccgcgatc tccgtgcccg ggaccggctt
     4921 ctgttccagg atcgtccccg ccggggcccg gtcgaagatg tagaccggcg gttcccggat
     4981 ggccaggagg ggccgggtgg aggagaagag tgtctgcagg tggatcttga cctcgtccag
     5041 ggtctggccc acgtagttct ccacctggtt gaccgcgggt ccccggctga cggtcaggtg
     5101 gatgcgccgc ccggccttga cgatggtgcc cgcgggaggc ttctgctcca ggatccggcc
     5161 ccggtccgtg gggttcgagg agaaccgcag ggcgatccga gggtagagtt ccttctcctg
     5221 gagcttgatc agggccgtgg ttagttccag gtccgtgacc tcgggaacca tggtctgctc
     5281 cgccccctgc aaggccagga agaaggcgat gaggccggat accgtcatga ggacgatgat
     5341 gcccaccagg gccaggaggg tgaatttgtg ggcgacgggg tccagttctt cgaggcgctt
     5401 gatgtcgatc ggcagtttca tgctcggtcc cggattcgag gattcgaccc tcggccccgc
     5461 acggtaccgg atgtcagtgg aaacgagccc ccacgaggtc tctcacgccg ttcgcgaatt
     5521 cccggaacgg cagaggtttc ttcgtctgga gctggaggcg ccgcagggcc aggaggccgt
     5581 tccccgtttg taccattatt ccccgagact tgtctagggc gaccacggtt ccgggatccc
     5641 gtcggcccgc ggacccgttc cccccggcgt cgagggcgcc ctccgggaag ggctccgact
     5701 ccaggaggtt cacccgctgt ccccgcagct ccgcgtaggc ccccggccag ggatggaagg
     5761 cccgtatccg ggcgtcgatg tccggggccg gaagggccca gtccacgcgg ccgtcctcct
     5821 tgcgcaacag ggagcagtag gtcgcctgcc cctcgtcctg ctcctcctcc gtcgcctcgc
     5881 cccgctcgat gcgctggagg accgaggcca aaaggtcggc tccccgctcg gccatcaggg
     5941 cggacagggt ctccgtggtc tcccggcccg agaggggcac gacttcccgg gccaggatag
     6001 cgccgcggtc catctcgagg gccaggcgct ggaccgtgac ccccgtctcg ctttcgcggt
     6061 tcaggatggc ccaggggatg ggggcgcagc cccggtaccg gggcaggagg gaagggtgga
     6121 cgttcacgcc cccccggggg aagagggcca ggaacttcgg accgaagatc cgtccgtagg
     6181 cgaagcacgc caggatctcc ggcttcaggg ccgccgccgc ctcccgggcc tcggctccca
     6241 gcctctcgaa agccaggatc ggggtctccg ggatcaggtc cgccgcggcc tcggccacgg
     6301 gggtccggac cgcacggagt ccccggcctt ttgccgcttc cgggttggtc aggaccccca
     6361 ccacgtcgtg gtcccggacc acagccgcca gggcgggcac ggctatttcg gggcttccgg
     6421 cgaacaggac tctcaagggc gcctcacatc cggatccggc gctcgtactg ggccaggacg
     6481 cgcttgcgcg cgggttccga gagccggtcc acgaagagga ccccgttcag gtggtccagt
     6541 tcgtggagga tgacccgggc ctccaggccc tccgcgtcca ggttgaaggg acggccgcgc
     6601 tcgttccagg cctggatcct caccgcctcg gggcgcttga gcttggcgta gaccccgggg
     6661 atggacaggc agccctcctc cagatccgcc agctcctggg aggtcagtac gatctcgggg
     6721 ttgatgaaga tccgggggcg ctcgtccacc tccgtcacga agagccggag ccccaggtcc
     6781 acctgggggg ccgccaggcc gatacccttg ccgacccgca tggtttccag catctcccgt
     6841 gccagggacc ggatcttgtc gtcgatatcc ttcacgggct cggcggtccg ggtcaggacc
     6901 tccctgccca gggtcacgat ttccagcatc ctcttcctcc gggacgtcgc ggtttcgtca
     6961 cagggttcgt gacaccctgc ggagcgcgtc cccagttaca tagtacgaac ggaagtcccc
     7021 gcggtcaaac cgtcccgctc tagcgggacc ggaccgagtc cggcaggatc acccggtccc
     7081 cgacccgtac cccggcccgg gagaaccagc cctgcgggac ctccagggcg taccgcaccg
     7141 agtactggga gggcaccggg gccagggacc ggggttccat gtccaggatc tcccggatgg
     7201 tcccgtccga ggagatgtag gctatggaga ggggcaccag ggtgttcttc atccagaagg
     7261 agagacgctc gtccgtgtcg aagacgaaga gcatcccggc gccgtccgga agggccttgc
     7321 ggaacatgag gcccagttcc cgttccccgg gggtccgggc gatctccgcc gtgacctggg
     7381 cggggccgac ggtcaaccgc tcggtccgga ggggcgggtt gcccttctgg gccgcgcagg
     7441 acgacagggc cagaagcccg gccgccagga ccgccaggat cgatttaggg tttctcatcc
     7501 cacgcctccg tgctttccga gctcgacctt ccggccgcgc ccggtcaaaa aaaaggcctt
     7561 gaaggcgtac cctcaaggcc gtgttcgcgt gcctatggat gcaaggccgc ttccgggtcc
     7621 cggatcagta cttgccgtcc cgggacttgc gcgttttctt catcagcttg cgctggaggg
     7681 ccttctgctt gcggttcttg atgagggagg gcttctcgaa gaactcccgc ttcttccatt
     7741 cgcggatgat gccttccttc tcgaccatgc gcttgaagcg cttgatggcc ttctcgaggg
     7801 gctcgctgtc gtcgacgatg atctggctcg tgggaatcac ctcccttcct tgtagaatgc
     7861 ggagattgta ccggcccggt ccgggaaggt caaggtccgg cagggaagac ggcgaaggcc
     7921 ggcgtctcgg ggtcgcaggc gacgccccgg acccggagct cccagtgcag gtggggtccc
     7981 gtggccaagc ccgtggaacc gaccgtcccc agccgggctc cccgctccag gaccgctccc
     8041 ggggcgacgg aaagtgaatc caggtgcatg tagacggtgt agactccggg gaggtgctcc
     8101 aggaccaccg tcttgcccgt gacctcgcgg tccgcggcca ggaccacccg gccccggccc
     8161 gcggcgacga ccggggttcc cgtgggcgcc gcgaagtcga tccccgcgtg gaccgcggag
     8221 gccgtccccc ccgtggcgta aaggtagcgg cgccgatccc cgaaacggct ggtcctgcgg
     8281 tccgaggcca cgggccgaag gaagggtccg tccagccaga ttccgtccgg atcgacgcgg
     8341 cccaggatat ccgcgtagac ccgggcttcc tcgtccttcc gggggctggg ctccacccgc
     8401 agggtcgtaa ggttcgtgga gagggggatg tcctcggcca ggaagtcccg gggacgtacg
     8461 gtcaccgagt gccgggagag ggcgggcagg attccggggc cgccggggat ttcgacggtg
     8521 tagatccccg ccggggcgtc cgtcggaagg ccgaacacga aggccgtccg gcccggctcc
     8581 ggacccaggc ggaagccggc ggcccgctga aggaccgtcc ccgccgggga cttcaggacc
     8641 gcctcgcccg cgggcgtccc ggattccggc cggggaagcc ggaccagcag gacatccccc
     8701 ggccggatct cggcggggag cgccgggagg gggatcgcgg cgggagccgg ggcctcctgc
     8761 ggggaaaccg gttgggccgc ctgcggggaa accggttggg ccgcctgcgg aatcgcgggc
     8821 gggaccgcgt cgggggcggg ggccggcggg ttagccccgg cgtcctgggc cgcggacgcg
     8881 cagagcaggg ccagggagag cagggcggaa aggaaccgtc gcataccggg actctacccc
     8941 gttccgaggg accgggacaa gcggtcggat ccgccgggct tgccctagtc gaagaggcgg
     9001 tgtaccctcg agtcatggaa ctgcccttca cccttacgga aacccgggac ggacgtctcc
     9061 ggtacgggat cgccctcggg taccgggtcc tggcggcgct catcttcggg atcctgtcct
     9121 ggaccctgta tgcccaggcc gaggcccctt ccgtcgtggc ctggctgctc ctggcccttt
     9181 ccgccttcgg ggtactgtac gaggagcggt ggattttcgg acccgccggg gcggagcacg
     9241 ccgtggggat cctcttcgcg gcgcgcactc tccggctgga ccgggaagcc ctggaagccc
     9301 tccgtctggt accccatgtc cacggtaccg tgccgggctc ggaggacgag ggccgggaaa
     9361 acgcccgcgc gctttcgggg gagcgggacg cggatcccga cggccggaag aaatctctgt
     9421 ggaaacgtcc gtatctcatt ctcgtcctgg cggcccggga cggctcggaa tacgccgtgg
     9481 acaaggtccc tgcccggaac cgggatcgga tcctggggta cgcccgcagg atcgcggagc
     9541 ggaccggact cgaactgaga tcctgaaggc gacgaaggcc gctttcgcgg ccttcgtcga
     9601 ttttcttaaa ctccggtccc tccgaccggt taaaatccgt caatccggtt cgtggcttcg
     9661 ggcgctaagc gcccgaaggc aacgaagggg cgcctttgcg ccccttcgtc gactcttcta
     9721 actccagcgc ggatcatacc gcacctgagt ttacgactca atccggttcg tgatagagga
     9781 agcaagctac ctggtggtcg ggaccggagt ccttcaggcg cgggaggttg tcccggcact
     9841 tgtccatccg cttggagcag cggtcgtaga agtagcagcc cgtccgggcc ttgtccgggg
     9901 acggcacgtc gccggtcagg atgatccgtt ggcgcttccg ctcgatcttg ggatccggga
     9961 tgggggccgc ggacaggagg gcctgggtgt aggggtgcat cggcgacttg tacagctcgc
    10021 tcgacttggc ggtctccacg atcaccccca ggtacatgac cgccacccgg tcggagatgt
    10081 actggatcac cgacaggtcg tgggcgatga acaggtacgt gaggccgaac tcttcctgca
    10141 ggtccttgaa gaggttcagg atctgggcct ggatggagac gtccagggcg gagacgggct
    10201 cgtcgcagag caccagctcg ggcttgaggg ccagggctcg ggcgatcccg atgcgctggc
    10261 gctgtccgcc ggagaactcg tgggggtagc ggttcttgaa gaaccgggaa agcccgacgc
    10321 gctccatgag ctgttccgcc cgggcgtcca tctccttgtc cgagatgtct aaaaggcccc
    10381 tctgcttgaa gatgcgcagg ggctccgcga tgatggatcc cgtggtcatg cgggggttca
    10441 gggaggcgta ggggtcctgg aacaccacct gcatgttctt gcgggctgcc aggaggtcct
    10501 gcttcgaggc cttcgtgagt tcctggccct tcagcaccac ctccccggcg gtgggcttgt
    10561 acagctgggc tatggcgcgc agggtggtgg acttgccgca gccggactcg ccgaccaggc
    10621 cgagggtttc ccccttgcgg atcgagaagt ccaccccgtc cacggcgtag atgaagccct
    10681 tcttccggtt ccagaggaaa ccctggtggg tggggaagtg catcttgagg ccccggacct
    10741 ccagcaggtt gttgtcgttc atgctatttc ccctccgtgt agagccagca ggccacgttg
    10801 cgccccttcg acagctccgc cgtcgcggga tacttgatct tgcagatgtc cagggccctg
    10861 tcgcagcggg ggtagaaggg acagcagtcc ggcaggttga tcacgttggg cggctggccc
    10921 ttgatggaga agagcctctg ctgggtggcc ttgtccaggc gcggcacgct cttgatgagc
    10981 ccctgggtgt aggggtgttt cgggtccccg aagatctcgt ccgcggtgcc ccgttccacg
    11041 atgcggcccg cgtacatgac gttgatgttg tcgcacatcc ccgccaccac ccccagggag
    11101 tgggtgatca ggatgaccgc cgtgcccaac cgggccgtaa ggtccttgat cagctccagg
    11161 atctgggcct ggatggtcac gtccagggcc gtggtgggct cgtcggcgat caggacctcg
    11221 gggttgcagg acagggccat ggcgatcatg acccgctggc gcatgccgcc ggaaaactgg
    11281 tggggatagc tgtcgatccg cttctccggg gaggggatgc ccgcgagctt gagcatctcg
    11341 atggacttgt ccttggcgtc cttcttgttc atcccctggt gaagcacgat ggtctcgatc
    11401 atctgggtcg agatccggag gaaggggttc aagctggtca tggggtcctg gaagatcatg
    11461 gagatcttgt tgccccggat cgtccggatc tcgtcgtccg gcatcttgag gaggtccttg
    11521 ccctggaaga ggacctcgcc ccccgcgatg cgccccggcg gcacgggaat cagcttcatg
    11581 accgccaggt tggtcacgct cttgccggaa ccggattcgc ccacgatccc cagggtctcg
    11641 cccttgcgca ggtcgaagga caccccgtcc acggccttga ggagcccctc gtcgagcttg
    11701 aaatatgtct tcaggtcctt gacctgcagg atgacgtcat cggtcattcg gttctctcct
    11761 tggggtgtta gccccggttt ttgctctggg gatccaacgc gtcccgcagg ccgtcgccca
    11821 ggaagttcat gcagaacagg aacaccgtca tggagatggc gggcacgaag agctgccacg
    11881 cgcggagctc catcccccgc accccctcgc tgaccagggt gccccaggag gcctgggggg
    11941 cggagactcc caggcccagg aaggagagga agctctcctg catgatgaag ctgggcatga
    12001 ggagggtgga gaagacgatg atcaccccga gggtgttggg aaggaggtgc cggaagatga
    12061 tgcggggtgt gccggcgccc atggagcggg cggcttccac gaactcgctg ttcttgaggc
    12121 tgatgatctg accccgtacc acccgggaga tggtgagcca ggagatgagg gcgatggcca
    12181 cgaagaggat gacgatgctc ccccggccct tatcccccac catggacata atgatgatga
    12241 ccaccagcat gtagggcagg gagtacatga tgtccacgaa gcgcatcagg aagttatcga
    12301 tcgctccgcc cacatacccc gagatcgacc cgatcaggat gccgatcagc accgccgtca
    12361 gggttcccac gatcccgatc atgatggaga tccggcctcc gtagatggtc cgggccagca
    12421 tgtcccgtcc caggtcgtcg gtgcccagaa tatatacctt ggagtgctgg ggatccgtat
    12481 ggatcgatcc ccggagagcc tcgacctcgg ccttggatcg ctccaattca tccacgacgg
    12541 agtccgagta ggtcggcccg tagtaggtgt cgtccgcttc ctccgtcact tcctcgaggg
    12601 ctgcctcccc ctcgtccacc tcgccgtcgt cgaactcgat ctccgccgcc ggggcgaaat
    12661 cgaagcccgt ggccatgtta taggtgctgt tggaatcctc ctcacgggag gccaggtttt
    12721 cctccagctg ggcgatgcgg agctccaggc tctcgatcac cagttccccg gccggtcgga
    12781 acgaggggcg caggttctgg tgggagatga tctgctccgt gtagctgtgc agaatgcccg
    12841 cggattgcag gaggggagcg gagagcgcca ggaggaagta gaatccgacg acccagaggg
    12901 ccaggacggc catcttgttc cttcggagcc gcttccacgc gtccgcccaa agggagacgg
    12961 gcttgacata ctggttgaat tcgttgaccg cgtcttgggt cgtatcgata ttggccataa
    13021 gggctcctac ttgtacgaga tccgcggatc gagcaatccg tagacgaggt ctaccaccag
    13081 gttcgcggtg atcagcagaa gggagtagac gatgacatcg gccatgatca tggtgtagtc
    13141 ccggttgatg gcggcctgca cgaagggccg gccgaggccg gggaccacga agaccgcctc
    13201 gaccaccacg gacccggtca cgatgcccga aagcgccggc cccaggtagg tcactacggg
    13261 aaggagggcc cctttcagga cgtgcttgac gatgaccacg gattccttga ggcccttggc
    13321 ccgggcggtg cggatgtagt cggaccggag gacctccagg accgaggccc gggagagccg
    13381 ggcgatgtag gcgaaatagg ggaaggagag ggtcaccgcc ggcaggatga ttgctttcaa
    13441 tccgtgatcc gatagccatt ggcccggcgg cagccatttc agccagatcg cgaagaccat
    13501 ctgaagaatg ggtcccatca cgaacagggg gacggaaatt cccacgacgg ccacggacat
    13561 ggcggtatag tcgggccatt tgttttgttt caaggcggag acgatgccca cgttcacgcc
    13621 caggatcacg gccagggaga gggccgtaag gcctaaggca gtggagaccg gaagggtggt
    13681 gtcgatcatc tcgttcaccg accggtcccg gtaccgcagg gaggggccga agtcccagcg
    13741 gatgacgtcc cctaggtacc ggagatactg cttcggcagg ggttcgtcca tgtggtaccg
    13801 cttcaggatg ttctgcagga tggcttccgg caggtcccgc tcccgggcga aggggcctcc
    13861 gggagccagc cggatcagga agaagctgag cgtcacgatg atgaacattg tcgggacgag
    13921 gctcagcagg cgccgaatga agaatcgtgc cattcgggtg cactccttgt ggttaacgcc
    13981 ggatgggctc gggttcctcg gccggcgtcg gaatcggggg ataaaaaaag cggagtacac
    14041 tctcgtgtac tccgcttgtt tcgtcggatt ataaccctat cttcgcgata cggctagtat
    14101 ccttcgatct ccggccttac ttccggtaga tgtacttcca ggggtggctt cccagggggt
    14161 tcaggtacca gccgccccac ttggacaggt cgatcaggtt gttcgtgacg tagaagtaga
    14221 tcggcatgat cgcgtggtcc ttcatgatga ggacatcttc cgcctgcatg agggtcttca
    14281 tgcgggtcgc gccggactgg gtgcgggcct tgaagatcag gtccgtgtac tccttgctgt
    14341 tgtacaggcc gtcgttctgg gttccgccga tgatccacat gtccgagaag gtggacggat
    14401 ccaggtagtc gccgatccag ccggcccggg cgatgtcgaa gtcgtggctc tgggagcggg
    14461 tgtccaggta ggtgctccat tcctggttga tcagggtgac ggtgatcccc aggttgcgct
    14521 tccactcctc ctggatgaag gccgcgatcc gctggtggcc ggagctggtg ttgtacagga
    14581 tctggaaggt cgggaagccc ttgccgtccg ggtagccggc ttcggccagg agcttcttgg
    14641 cctgctcgat gttgtagccc aggccggcgg ggggcttgaa gcccgcgctg gcgggaacga
    14701 aggtgaaggc cgggatctgc ccgcccttgg tgaccttgtc caccagggcc tggcggtcga
    14761 aggccatgga cagggccttg cggaccttcg ggtcgtccag gggcttccgg gttacgttat
    14821 agatgtagta gtaggtcccg tagtacgggg cgttctggta gtccttccgg agcttgatct
    14881 cgtccatgag ctcgaggggg accgcgtcga gccagtcgac cttcccggcc ttgcacatct
    14941 ggtaggaggt ggacaggtcg tcgatcggca ggagggtgat cttctggagc ttgacggtct
    15001 tggcgtccca gtacttgggg ttcggcacga agaccaggcg ctcctggggc ttccactcct
    15061 ggaggaggaa ggggccgttg ccgacgaagt tgcccggctt gacccagtcc aggccgtgct
    15121 tcttcacggc gtgcatgggg accgcggcga aggcgtaatg ggccaccatg tcggggaagt
    15181 ggggggtcgg gccgaccagc ttcacctcga aggtcgagtc gtccacggcg cggatcttga
    15241 gggcctcggg gccggccttg ccggtgttgt aggcctcggc tccctcgacg acatacagga
    15301 ggtcggcgta ctgggcggct tccttcgggt cgagcttgcg aatccaggac tccacgaagt
    15361 ccttggccgt gatcggggtc ccgtcggacc agacggcctt ccggagcttg aaggtgacgg
    15421 tcttcaggtc cttggagaag gtccacttct ccgcgacgcc aggtacggcc cggctggtct
    15481 tgggatcgtg ggtcgtgagg ccctcgaaca aggccatgta gatccggtgc tcgggcacgc
    15541 cttcgatgtg gtgcgggtcg agcgtctggg gctcggcacc gttgttcatg atgatctcct
    15601 ggcccttgct ctgggcgccg acgatcccgg ctaccgcgag caacgcgagc ataaccaaca
    15661 gaacacgttt cattcatgcc tccttatggc attagaaaga gtgggtatat tgtacgctca
    15721 ccgtcctgaa atgacaactt ttttttcaag ccccggagtc cggattcgga aatcttgccg
    15781 gctttatagt aaatccttcc tgccctggga tttaagtctc tccacggcct cccggaccag
    15841 ctgggaggac ccctttttca gcaccagggc ggccccgttc cggatgacca cgaccaggtc
    15901 cgagaccccg cagagggcca cggggatgtc ggagtcgacg tagcaggcct cggattcgac
    15961 cgccacggcg ttgccggagc ccccggggaa gagccgggcc atctcgtccc aggatcccac
    16021 gtcctcccag gtgaaggagg ccgggaccag ggccacggag gcgcacttct cgctgaccgc
    16081 gtagtccagg gagaccgagg gagcccgttc gtaggcttcc tcgaggcctt cccaggcctc
    16141 cacgatccgg acgccccccc gctcggcctc ccgggggcgg tcgatcaggg agtcgaaggc
    16201 ccgggccacc tccggggcat gggcggccaa ttcccggcgg aggaagtccg agcggaaggc
    16261 gtacatgccg ctgttccagt agtacttgcc cgcctccagg aagcgcgcgg cggtggcggc
    16321 gtccggcttt tcccggaagc ccgcggcccg gaatcccggc cccaggggcc cggccgcttc
    16381 cacgtagccg taacccgtgg cggggtaccg gggcgggatg ccgaagcaga ccaggttgcc
    16441 cgcggcggcc agggcttccg ccttggccgc gtccgccgcg aaggcggaag cgggctcgat
    16501 gaggtggtcg ctggtcatga gcaggaaggt gtcctccggc cggccgcccc aggcttccgc
    16561 ggcggccagg gcccaggcca cggcgggggc cgtgttcctg cccgcgggct ccgggaggat
    16621 tcgcacggag ccccgtcccg gggcgcccgg gggcagggcc cggcactcct ccgccacggc
    16681 ctcggccatg gaagcgcccg tgacgatcag gatcccccgg gcgggggcca gggccaaggc
    16741 gcggtccagg gcgcggcgga agaagctgga cccgtccccg agggccagga actgcttggg
    16801 gcgggcggag gtggaggccg gccagagccg ggtcccggag ccgccggcca ggatgatcac
    16861 gtaggaatac atgggaatac tatacgacga gggaggcgcc gggccaagtc ccgatccgcc
    16921 gccgggcgtc cggtttgtcg accccggtca acgaaaccct ccgcctccgc ggatccggag
    16981 tccccgtatc tatggtagaa cacggttttc cggaaaaaaa tacgcatatt tat
//
LOCUS       DAOTEI010000067        16927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2298_length_16927_cov_5.896278, whole genome shotgun sequence.
ACCESSION   DAOTEI010000067 DAOTEI010000000
VERSION     DAOTEI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 16927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16927
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_16927_cov_5.896278"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..724)
                     /locus_tag="P5313_08155"
     CDS             complement(29..724)
                     /locus_tag="P5313_08155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY80374.1"
                     /translation="MAFRAIGFDIDGTLYPSWRLNLALAGFAARNYRCLKAFGRVRHE
                     LRELARTPEYRAAPPAGIEEFHRLQAGLVARRMGIDDQTALEGVERFFYRLVPESFDR
                     IRAFPGVRQTLLRLKAAGLRLGALSDFPGERKLELLGLDGLFDVVMTSEETGYLKPEP
                     EPIRILAGRLGVESRDLLYVGNSAAYDVAGAKAAGAPSAYVRTLLGGRKDGGADFSFR
                     RYPDLADWILSRP"
     gene            complement(961..2724)
                     /locus_tag="P5313_08160"
     CDS             complement(961..2724)
                     /locus_tag="P5313_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRY80375.1"
                     /translation="MEQNMVQLTIDGRGVSVPAGTRILDACAAAGVRVPTLCHLPEVS
                     SNASCGVCVVEVEGAKSLVRSCVQNVGPGMVIKTNSPRVQESRRTIVELLLANHPADC
                     LSCARNGSCELQALSDQMGVRRLPFPRTRKKPGLDDAGYSLVRDNDKCILCGRCVAVC
                     RETQTVSAIDFAGRGIRTRVSSFMDRSLANSQCVSCGQCTVVCPTGALVERDETDEIF
                     AALADPDRVVVVQTAPAIRASLGEALGMKTGSLVTGKMAAALRRLGFDKVFDTQFTAD
                     LTIMEEGSELISRLTNGGKLPMITSCSPGWITFIETFYPSLLPHLSTCKSPQQMFGSL
                     AKSYWAEKAGVDPSKITVVSIMPCTAKKFEAKRPEMRDAWKWWKEKGKTYGSFFDVDY
                     ALTTRELARMIRRAGIDFELLPDEDFDDPLGASTGAATIFGTTGGVMEAAVRTAYELV
                     TKKPFPVMELQATRGMEGIKSAELDLEGTKLKVAVAHTLKNARAVLEEIAAGKSPYAF
                     VEVMTCPGGCIGGGGQPVAVDQKKRATRQKAVYEEDKRLPIRKSHENPSISAIYKEFL
                     GKPLGHLSHELLHTEYKRRDV"
     gene            complement(2736..5924)
                     /locus_tag="P5313_08165"
     CDS             complement(2736..5924)
                     /locus_tag="P5313_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRY80376.1"
                     /translation="MKPIKSMADLEAAKARGLSKLVPATTRISVGMGTCGIGNGADLL
                     FAALEKAAAKAAGKNGMAPVLKKVGCFGFCAAEPLLTLAVPGKPVLLFTKADAKDAAA
                     FVEAASDPKALDKLAKKADGKIETWDFRTSVLRFGEGFPEVRSWNQIPFFEGQEKVVL
                     RDAGIIDPEDVEEYLGAGGYGALAKAVLSMKPADVILEVDRAGLRGRGGAGFPTARKW
                     KMMADRPGPEKWVVCNADEGDPGAYMNRNEIESDPHALVEGMILGAYAMGANKGFVYV
                     RAEYPLAVDRLRKALDDARALGFLGKNILGTDFSFDLEIVMGAGAFVCGEETALTSSA
                     EGKAGRPVPRPPFPAEKGFKGLPTNINNVETWCNVPAIVSKGGAWFAGIGLPKSTGTK
                     VFSLVGKVNNTGLVELPLGTKLEKMVYGMGGGAGMNKAIRAVQSGGPSGGCIPADKFD
                     ATIDYESLASIGAIMGSGGMVVMDQDNCMVDVARYFVSFTAAESCGKCAPCREGLSQM
                     LRILTDVSKGTATYADLDELERLARTIRDSALCGLGQTAANPVLTTLKYFREEYERHI
                     RDKRCDAGTCEQLFCALCENSCPMHMNIPGYLALLKEGRLEEAYELTLRDNPLPGTIG
                     RICHFHCSMRCRRETLDDPVQQGEIHRYLADTVRKMGREQAVWKRLADEKAKPTGKKV
                     AIVGSGPAGLAAAFYLVRLGHEVTIYEDKEKAGGILRYGIPAYRLPKDELDHELQLFS
                     KLGIKFVFRTRVGRDIALADLAKKADAVLVCVGAEGDKALAIPGEDLQGVHPGYEYLD
                     LHAAKKAPKTGRRVLVIGGGNVAIDAARTLYRMDKDVVVAYRRTRADMPANRAEIEGA
                     EEEGIRFRFMLAPQEILGDGKGRVRAVRFEIMKAGPIDRSGRPTPVSTGHFEDIPCDD
                     VIVAVGEKVDSGDFVKDGIKAAKDGRILANPFTLKTDLPNVWATGDAVTGPATAAEAM
                     GDAKRVAREMDKALTGEDRFTTLFRDFHYNMEVPVEPEGSRKNRPRHLPPDQRVGNFI
                     EINLGYDGDQAISEARRCLRCDVRPDARSPWR"
     gene            complement(5921..6427)
                     /locus_tag="P5313_08170"
     CDS             complement(5921..6427)
                     /locus_tag="P5313_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY80377.1"
                     /translation="MDKSDPRTGLQGIVQRYAGRPGALLGILEEAQQADEHKYLSEEA
                     LRQIADMTDTPLSRVYSVATFYSFFNLKPQGKHAIVVCRGTACHTRGSLALLLEAANR
                     LGFPEFREDEEPSFTTEDRMFTVRTVACFGQCALAPVVAVDGVIYSHMNTARLLSILE
                     TLRKGVRK"
     gene            complement(6674..7051)
                     /gene="crcB"
                     /locus_tag="P5313_08175"
     CDS             complement(6674..7051)
                     /gene="crcB"
                     /locus_tag="P5313_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRY80378.1"
                     /translation="MPDVILVVLAGGLGSALRYAFSVGANRLIGPSFPWGTLIVNLAG
                     CLAIGFAAGLVDRSILPRAWRLAVITGFLGGFTTFSTFSLESLRLILDGAVFRGLANI
                     GINLLGGLALAALGLRMAARFGR"
     gene            7170..7808
                     /locus_tag="P5313_08180"
     CDS             7170..7808
                     /locus_tag="P5313_08180"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRY80379.1"
                     /translation="MKLVFLGPPGAGKGTVAEKAQKDLAVPHVSTGEIFRAAVKNGTP
                     LGLRVKAVIESGALVPDELTIELVRERLGQGDAKSGWILDGFPRTIPQADALEGFDPP
                     DRVVDFELGDPAIVERLSGRRTCPACGRSFHVKFMPPAKAGICDSCGGTLITREDDRI
                     EAIQRRLSAYRAQTAPLIQYYRGKGRLLTVDASPEADAVYAAFLGLPGMNKR"
     gene            7978..13578
                     /locus_tag="P5313_08185"
     CDS             7978..13578
                     /locus_tag="P5313_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRY80380.1"
                     /translation="MRKPSFRYGFRRALAGYAAVAAVLCALCLLASCGAAVPTAEVPA
                     DPGLVAAHSAGTVSRHGEILVVLARNYGFQSGDSPVRNPFRLDPGTEGTAYWKDGSTL
                     AFKPSAPLAAGGIYTVSVDLASVDAAAREPFSFRIRTPEPALTVELGDLRAARDGTLE
                     LPGRVVLSDAEASDKVERLLSARFRGLKPGIAWTHGGAVHDFTVRGFSAADRDADLEL
                     SWDGRPVGAASRGGRSVRVPAAGSFEVTGIRAVREGESFLEVSLSSPVDPGQDFRGRI
                     EVEGIPDLRFSAAGGIVRVYASAAWPEEARVRIGEGLRDSEGRSLAVPVQATVPVSWE
                     KPAVRFATAGVVLPTSEGLTLPIETRNLAGVVVEVFRIPGDNMLQFLQVNKLDGDDEL
                     YRTGEVVWRKAFDLPWSDTRRNQWTAAGLDLGPLLAKYKDGMYRIRLTFLRDQVRYVC
                     RTEHDFKDLAFPTLETLDRHWDGDWEARYKFRDDPCHPAFYERNSTYRNVLISDLALG
                     AKRDADGRTGIFAVDLRSARPVPDLEVILYNFQRRELARGRTDKEGFATFPASREAFS
                     AVAFRSGQAAYLKLDPGSALAVSHFDAGGTASESGVKGFLYGERGVWRPGDEMHLTFL
                     LLDRLKTLPAGHPVRFELENPMGKTVQTSVLSEGTDGFYPFKARTAEDAPTGTYLARV
                     TVGDRIFTRNVKVETIVPNRLKIEFDFGGGAYLGDGEARFSLKSQWLHGAPAPGLKAD
                     VSVAFSPAPTTFPGFGDYVFEDPTRRLATERQTIFDGRLDTEGAVSFSSAFDAAGSAP
                     GAATAQFAVRVFEPNGFFSSEQFTVPYHPYARYVGVRLDTRWGTVKADQDQTVKLALV
                     DRDGKPVSAQNLEVGIHRIRENWWWEGGEDSLPEYARSIFERSVLKGQATVRDGRGSW
                     TFKVNQSDSWGIYLIRVRDPVSGHSASQVFWVSRYDWYGRPGEKAAPSNLALTASQER
                     YVPGQKVRVSFPSNSKGRALVTVERSGRVLSQKWQDCAEGTTTVEFTASAEMSPNAYL
                     HVDFVQPHAGRGNDLPIRLYGILPILVDDPATALAPRIEAPESFEPNKPASFRVREAS
                     GKPMTVTVAVVDEGLLGITRYKTPNPRDEFYKKEASALRAWDIFDFVTGAYSGKLETL
                     LAVGGGDGGELEGGRKPSRFPPVVRYFGPVTLKAGETRTFTTDLGPYVGAVRVMAVAG
                     RSDGAYGVAEVPVPVKAPLMVQATLPRVLSLEEWTSVPAAVFSSLGPGASVTVRISAS
                     GAAEVEGEAARTIVFDRDGERNTEFRVRALSVPGTARIVVTAEGGGKRSEHAVDLPIR
                     PAGAKAAADWEAQASPGGVAELSLAYPGMPGTHKVWLELSPLEPIQLTRRVNYLIGYP
                     HGYAEQTISKAFPQILLPEAANLSEWELEDVRTNVRAAIAKLSDYQAPSGGLALWPGS
                     GREDDWVSVYAAHFLLTARAAGYEVPAGLVEPLWKHLDARAVAWGSQYRWAKATQAYR
                     LLVLARAGKPNPAAMNRLRDAGNLPTQALWQLAAAYALSGQRGTAADLVKGLDPRIEA
                     ARSLEEDYYYYVYGSDFRDAAVILDALNALGDEARAAGLYRDIAKRMNSAYWFSTQET
                     AFALLAALPYSRRIAGAGASAARWSYNGSPEARIALTKPVTRVALPAGTEAGGRLTVR
                     AEGGAPVYVRVLAEGEARAGEEAPEARGLSVLTVYKDMEGRTLDPEAAPPLSDLVVET
                     TVRNLTRRTVRNVALTQKLPSGWEIRNFRVGSEERETWDEGYGQGSGRPWWYPRRDYT
                     YQDVRDDRVFTYLDLGPGKEAVVRVFANKTYDGRFYRPGAFAEAMYDPEYRALERGAW
                     MRSWEEASSGARNKPTVNSTLRSK"
     gene            13575..15911
                     /gene="pbpC"
                     /locus_tag="P5313_08190"
     CDS             13575..15911
                     /gene="pbpC"
                     /locus_tag="P5313_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRY80381.1"
                     /translation="MSRRESAPVAAGAVLLAALVLLVPGPPRFPGGYSTVLYDRNGLL
                     LGAAAAPDGQWRFPPAPDLPDRYVRALVAFEDRRFFGHAGFDPRALARALVSNLAAGR
                     IVSGGSTLSMQAARMARGNRPRNLGQKALELLYALRLEAFRGKRGILALYAAHAPFGG
                     NVVGLAAASWRYFGRPPEDLTWAEAAVLAVLPNSPALVHPGADRDRLREKRDALLMRL
                     AERGELGPEDLAAARAEPIPDRPEPLPRKAPHLLSRAVRDGLAGTRVDSTLDGGLQDL
                     VARTAARHGARLASRGIRNAAVIVAEIESGEILAYLGNLDGPEGGEDREAARWVDCAA
                     APRSTGSILKPFLYAALLDGGDLSPTTLVPDLPTSFGSFSPENNTRTYSGAVPAERAL
                     AHSLNVPFVRLLRTYGVDAFLALLKGYGFSELGRTAGAYGLTLVLGGAEATLWDSASA
                     YADLARSARGLEGVPAFAYSRTPPAPRGSGSVPASPGAAYLTLRALLEVGRPAEESAW
                     KDWAEGRRIAWKTGTSQGFRDAWALGVTSEHLVAVWVGNATGEGRPELRGSEAAAPLL
                     FDVFELLPRDPWIDPPPDALRRETVCADSGYLAGPDCARTLRIDLPSGAPPPDPCPYC
                     RTIHLTADGAFRATADCVPLDRLVSRKVFVLPPALEWYFRAGHPAYRPLPPVLPGCGT
                     VEDIPVTVLVPEEGARLFIPVELDGTPGRAVFRAVHRDPRARLFWHLDGDYLGETTGP
                     EHRLEVRPARGRSVLTVVDQEGRTSTRRFEVLSEREGS"
     gene            15942..>16927
                     /locus_tag="P5313_08195"
     CDS             15942..>16927
                     /locus_tag="P5313_08195"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY80382.1"
                     /translation="MKAFPPRTRRSAFAVLWALSLLRLAAQDASWLGFWEGEFRTAQG
                     DFPARLEIFSGGALLDLPEQGAFGWPVRTLKAEGSDLYLSVELGMEPLELAGPREGEV
                     VAGTLIQGGRSGRFQLRRAPDARDRDGERSFDTGRGVLPGTLRLPPGAAGPLTLAILL
                     PDSGTADRDGNNWQVPGRNDSLRMLAESLAEAGIASYRYDRRGAGKAYRLGEDEAGLV
                     FEDHVRDAAACLRSFEGDPRFGRVLVFGLGDGALVGAAALREAKADALAALGVSGRSL
                     RDIILEAALRAPEEYRAEIVRVLGELDAGRFVDAVSPFLENLFRRSFQPYLAS"
BASE COUNT         2421 a         5995 c         5878 g         2633 t
ORIGIN      
        1 ggtctggttc gactgaagta gtccgcggct aggggcggga gaggatccag tccgcgaggt
       61 cggggtagcg tcggaagctg aagtccgcgc ccccgtcctt ccgccccccc aggagggtcc
      121 ggacgtaggc ggacggcgcg ccggccgcct tggcccccgc cacgtcgtag gccgccgagt
      181 tgcccacgta caggagatcc cggctctcca cccccaggcg gccggccagg atccggatgg
      241 gctcgggttc cggcttgagg tacccggtct cctcgctggt catcacgacg tcgaagagtc
      301 cgtccagccc caggagctct agctttcgct ctcccggaaa gtccgagagg gcgcccagtc
      361 gcagccccgc ggccttgagc cggaggaggg tttgccggac gcccggaaag gcgcggatcc
      421 ggtcgaagct ctccgggacc agccggtaga agaacctctc gacgccctcg agcgcagttt
      481 gatcgtcgat gcccatgcgc cgggcgacca gccccgcctg cagacgatgg aattcctcga
      541 tccccgcggg cggggctgcc cggtattcgg gcgttcgcgc gagctcccgc agttcgtgcc
      601 ggacccggcc gaaggccttg aggcagcggt agttccgagc cgcgaatccc gccagggcca
      661 ggttgaggcg ccaggagggg tagagggtgc cgtcgatgtc gaagccgatc gctcggaagg
      721 ccataggtta ccgtttatac ccgatgcctc tacaggctgt ccatgacgtt accttaaaaa
      781 atcggacagg atgtacagga taaaaaaaga tgtacaggat ggaacttagg tattgggata
      841 tcctgtctat ccctaatcct atcctgttca tcctgtccag gatcgaaaga aaaagcccgg
      901 ggccgagggg ccccgggctc ggatccgtcc cgccgcggga gctaggagtc cgacaggctc
      961 ctagacgtcc cgcctcttgt actccgtgtg cagcagttcg tggctcaggt ggcccagggg
     1021 cttgcccagg aactccttgt agatggccga gatcgacggg ttctcgtggc tcttgcggat
     1081 gggaaggcgc ttgtcctcct cgtaaaccgc cttctgccgt gttgcccgct tcttctggtc
     1141 aaccgccacg ggctggccgc cgcctccgat gcaaccgccg gggcaggtca tgacctccac
     1201 gaaggcgtag gggctcttgc ccgcggcgat ctcctccagc accgcccggg cgttcttgag
     1261 ggtgtgggca acggcgacct tcagcttggt cccctcgagg tccagctccg cgctcttgat
     1321 cccctccatg ccccgggtcg cctgcagctc catcaccggg aagggcttct tggtcaccag
     1381 ctcgtaggcg gtgcgcaccg cggcctccat gacgccgccg gtggtcccga agatggtggc
     1441 cgcgccggtg gaggctccca gaggatcgtc gaagtcctcg tccggcagaa gctcgaaatc
     1501 gatgcccgcc cggcggatca tccgcgccag ctcccgggtg gtcagggcgt agtccacgtc
     1561 gaagaaggac ccgtaggtct tgcccttctc cttccaccac ttccaggcgt cccgcatctc
     1621 cggccgcttg gcctcgaact tcttggccgt gcagggcatg atggagacca ccgttatctt
     1681 ggagggatcc acccccgcct tctccgccca gtaactcttg gccagggacc cgaacatctg
     1741 ctgggggctc ttgcaggtgg acaggtgggg caggaggctg gggtagaagg tctcgatgaa
     1801 ggtgatccag cccggcgagc aggaggtgat catcgggagc tttccgccgt tcgtcagccg
     1861 ggagatcagc tcgctgccct cctccatgat cgtgaggtcc gcggtgaact gggtgtcgaa
     1921 tactttgtcg aagccgagcc ggcgcagggc ggcggccatc ttgccggtca ccaggctccc
     1981 ggtcttcatg cccagggcct cgcccaagga ggcccggatg gccggggcgg tctggaccac
     2041 cacgacccga tcggggtccg ccagggccgc gaagatctcg tccgtctcgt cccgctccac
     2101 cagggcaccg gtggggcaga ccaccgtgca ctggccgcag gacacgcatt gggagttggc
     2161 cagggagcgg tccatgaagg aggacacccg ggttcggatg ccccggcccg cgaagtcgat
     2221 ggccgacacg gtctgggtct cccggcagac cgccacgcag cggccgcaga ggatgcactt
     2281 gtcgttgtcc cgcaccaggg agtagcccgc gtcgtccagg cccggcttct tgcgggtccg
     2341 ggggaaaggc aggcggcgaa ctcccatctg gtccgagagg gcctgtagct cgcaggaccc
     2401 gttccgggcg caggacaggc agtccgcggg gtggttggcc aggaggagct ccacgatggt
     2461 ccgtcggctc tcctggaccc ggggggaatt cgtcttgatg accatgccgg ggcccacgtt
     2521 ctgcacgcag gaccggacca gactcttggc cccctcgacc tccaccacgc agaccccgca
     2581 ggaggcgttg ctggacacct cgggaaggtg gcatagggtg ggcacccgga cccccgcggc
     2641 cgcgcaggcg tccaggatcc gggttcccgc gggcacggaa accccgcggc cgtctatcgt
     2701 cagctgtacc atgttctgtt ccatacgcgt cctccttagc gccaggggct gcgggcgtcc
     2761 ggccggacgt cgcagcgcag gcagcgccgg gcctcgctga tcgcctggtc gccgtcgtag
     2821 ccgaggttga tctcgatgaa gttcccgacg cgctggtccg ggggcaggtg ccggggccgg
     2881 ttcttccggc ttccctccgg ctccacgggc acctccatgt tgtagtggaa gtcccggaag
     2941 agggtggtga agcggtcctc cccggtcagg gccttgtcca tctcccgggc cacgcgcttg
     3001 gcgtccccca tggcctccgc cgcggtggcg ggacccgtga cggcgtcgcc ggtggcccag
     3061 acgttgggca ggtcggtctt gagggtgaag gggttcgcca ggatgcggcc gtccttggcg
     3121 gccttgatcc cgtccttcac gaagtccccg gagtcgacct tctcccccac cgccacgatc
     3181 acgtcgtcgc aggggatgtc ctcgaagtgc cccgtggaca ccggggtcgg ccgcccggag
     3241 cggtcgatgg gtccggcctt catgatctcg aagcggacgg cgcgcaccct gcccttgccg
     3301 tcccccagga tctcctgggg agccagcatg aaccggaagc ggatgccctc ctcctcggcg
     3361 ccctcgatct ccgcccggtt ggcgggcatg tccgcccggg tgcggcggta tgccacgacc
     3421 acgtccttgt ccatgcggta gagggtccga gcggcatcga tggccacgtt gcctccgccg
     3481 atcaccagga cgcggcgtcc cgtcttgggg gccttcttgg cggcgtggag atccaggtac
     3541 tcgtagcccg ggtgcacgcc ctgaaggtcc tcgcccggga tggccagggc cttgtcgccc
     3601 tcggcgccca cgcatacgag gacggcgtcg gccttcttgg ccaggtcggc cagggcgatg
     3661 tcccggccca cccgggtccg gaacacgaac ttgatcccca gcttggagaa gagctggagc
     3721 tcgtggtcca gctcgtcctt gggcaggcgg tacgcgggga tgccgtagcg gaggatgccc
     3781 cccgccttct ccttgtcctc gtagatcgtg acctcgtgtc ccagccggac caggtagaag
     3841 gccgccgcca gacccgcggg gccggatccg acgatggcca ccttcttgcc cgtgggcttt
     3901 gccttctcgt ccgccaggcg cttccagacc gcctgttccc ggcccatctt ccggaccgtg
     3961 tccgccaggt agcggtggat ctcgccctgc tggaccgggt cgtccagggt ctcccgccgg
     4021 cagcgcatgg agcagtggaa gtggcagatg cggccgatgg tgcccggcag gggattgtcc
     4081 cggagggtca gctcgtacgc ctcctccagc cggccttcct tgaggagggc cagatagccg
     4141 gggatgttca tgtgcatcgg gcaagaattc tcgcagaggg cgcagaagag ctgctcgcag
     4201 gtgcccgcgt cgcagcgctt gtcccggatg tgccgctcgt actcctcccg gaaatacttg
     4261 agggtcgtga gcaccgggtt ggccgcggtc tgccccaggc cgcagagggc ggaatcccgg
     4321 atggtccggg ccaggcgctc cagttcgtcc aggtccgcgt aggtggccgt gcccttggac
     4381 acgtccgtca ggatgcggag catctgggac aggccctccc ggcagggggc gcacttgccg
     4441 caggattccg cggcggtgaa ggacacgaag taccgcgcca cgtccaccat gcagttgtcc
     4501 tggtccatga ccaccatgcc gccggagccc atgatggccc cgatggaggc cagggactcg
     4561 tagtcgatgg tggcgtcgaa cttgtccgcg gggatgcagc cgccggaggg cccgccgctc
     4621 tggacggccc ggatggcctt gttcatgccg gcgccgccgc ccatcccgta gaccatcttc
     4681 tccagcttgg tgcccagggg cagctccacc aggcccgtgt tgttgacctt gccgaccagg
     4741 gagaagacct tggtgcccgt gctcttggga agcccgatcc ccgcgaacca ggccccgccc
     4801 ttggagacga tggccgggac gttgcaccag gtctccacgt tgttgatgtt cgtgggcaga
     4861 cccttgaagc ccttttccgc ggggaagggg ggccgcggga ccggccggcc cgccttgccc
     4921 tcggcgctgg aggtcagggc cgtctcctcg ccgcagacga aggccccggc gcccatgacg
     4981 atctccaggt cgaaggagaa gtccgtgccc aggatgttct tccccaggaa gcccagggcc
     5041 cgggcgtcgt ccagggcttt ccggagcctg tccaccgcca ggggatattc cgcccggacg
     5101 tagacgaagc ccttgttcgc gcccatggcg taggcgccca ggatcatgcc ctccaccagg
     5161 gcgtggggat cgctctcgat ctcgttgcgg ttcatgtagg cgcccgggtc gccctcgtcg
     5221 gcgttgcaca cgacccactt ctcgggcccg ggccggtcgg ccatcatctt ccacttacgg
     5281 gccgtgggga agcccgcccc gccccggccg cgcaggcccg cccggtccac ctccaggatc
     5341 acgtccgcgg gcttcatgga caggaccgcc ttggccaggg ctccgtagcc cccggctccc
     5401 aggtattcct cgacgtcctc ggggtcgatg atccccgcgt cccggaggac caccttttcc
     5461 tgcccctcga agaaggggat ctggttccag gaccggacct cgggaaagcc ttcgccgaag
     5521 cggagcaccg aggtgcggaa gtcccaggtc tcgatcttcc cgtccgcctt cttggccagc
     5581 ttgtccaggg ccttggggtc cgaggcggcc tccacgaagg ccgcggcgtc cttggcgtcc
     5641 gccttggtga agaggagcac gggcttgccc gggaccgcca gggtcagcag gggctcggcg
     5701 gcgcagaaac cgaagcaccc gaccttcttg aggaccgggg ccatgccgtt cttgccggcc
     5761 gccttggccg ccgccttctc cagggccgcg aacagcaggt ccgctccgtt tccgatgccg
     5821 caggtgccca tgcccacgga gatgcgggtc gtcgcgggca ccagcttgga aagcccccgg
     5881 gccttcgcgg cctcgaggtc cgccatcgat ttgatcggtt tcatttacgg acccccttgc
     5941 gcagagtctc caggatggaa aggaggcgcg ccgtgttcat atgcgaatag atgaccccgt
     6001 ctacggccac caccggcgcc agggcgcact gcccgaagca ggcgaccgtg cggaccgtga
     6061 acatccgatc ctcggtggtg aaggagggtt cctcgtcctc ccggaactcc gggaagccca
     6121 gacggttcgc ggcctccagc agcagggcca gggaaccccg ggtgtggcag gcggtgccgc
     6181 ggcatacgac gatggcgtgc ttgccttggg gtttcaggtt gaagaatgaa tagaaggtcg
     6241 cgaccgagta gaccctcgac aggggggtgt cggtcatgtc cgcgatttgg cgcagggcct
     6301 cctcgctcag gtacttgtgc tcatccgcct gctgggcttc ctccaggatt cccaggaggg
     6361 ctcccggcct tcccgcgtac cgctggacga tcccttgcag tcccgtacgg gggtctgact
     6421 tgtccatgac gcctcctata gatgcttgcc ccgagggaag gatccggatg caggacaggt
     6481 tgctgagcgc gacgctttcc gaagctcggg gctcgaacgg gataatttta taccggtttc
     6541 atggatttta caagaaaaaa ctatctataa atttaaatca tgactatggt catttgataa
     6601 aaaagggcgc cgacggtgat gggagggggc cggggcgcga cgacccggaa gccgggcggc
     6661 ctgacgcggg cgcctaccgc ccgaagcggg ccgccatacg cagacccagg gccgcgaggg
     6721 caaggccgcc caggaggttg atcccgatgt tggccaagcc ccgaaagacc gcgccgtcca
     6781 ggatcagccg cagggactcc agggagaagg tggagaaggt ggtgaaaccg cccaggaagc
     6841 ccgtgatgac cgccaagcgc caggcccggg gaaggatgga ccggtccacc aggcccgcgg
     6901 cgaagccgat ggccaggcag cccgcgaggt tcacgatcag ggtcccccag gggaaggagg
     6961 ggccgatgag gcggttcgcg ccgaccgaga aggcataccg cagggcggac ccgaggcccc
     7021 cggcgagcac cacgaggatt acatccggca tggggaaact gtagagtcct cgcggcccct
     7081 tgtcgaccac tcgggcagga tcccgttccc tcgttcggcc ggtcggatcc cgctttactt
     7141 ccgactttcg agggtatatc ctcccatcca tgaagctcgt attcttaggt ccccccgggg
     7201 ccggcaaggg cacggtggcc gaaaaggccc agaaggatct ggcggttccc cacgtgtcca
     7261 cgggggagat cttccgggcg gcggtcaaga acgggactcc cctgggcctg cgggtcaagg
     7321 ccgtgatcga gtccggagcc ctggtgccgg acgagctcac gatcgaactg gtccgggagc
     7381 ggctggggca gggggacgcg aagtccgggt ggatcctgga cggcttcccc cgcacgatcc
     7441 cccaggcgga cgccctggag ggcttcgatc ccccggaccg ggtcgtggac ttcgagctgg
     7501 gggacccggc gatcgtggag cgcctgtccg gccggaggac ctgccccgcc tgcgggcgca
     7561 gcttccacgt gaagttcatg ccccccgcga aggccgggat ctgcgattcc tgcgggggta
     7621 ccctcatcac ccgggaggac gaccggatcg aggcgatcca gcggcgcctc tcggcctacc
     7681 gggcccagac ggcccccctg atccagtact accgcgggaa gggccggctg ctcaccgtgg
     7741 acgcctcccc ggaggcggac gcggtctacg ccgcctttct cggacttccg gggatgaaca
     7801 agcgatagta agcccggcgg gcggggacct ctcacaatct taaggacacc gggtacggat
     7861 cccccctgcc ggaccgtcgt tccgcggtat aatccccctc gagcgaaccg tcgggccgcc
     7921 ccggtcgtgt ccgggcggcg ggccctaccc aataccacgt tcgaatcgga gtgaaccatg
     7981 agaaaaccat ccttccggta cggtttccgc cgcgccctgg ccggatatgc cgcggtcgcc
     8041 gcggtcctct gcgccctctg tctgctggcg tcctgcggcg ccgccgtccc gacggccgaa
     8101 gtccccgcgg atcccggcct cgtggcggcc cattccgcgg gcacagtttc ccggcacggg
     8161 gagatcctgg tcgtcctggc ccggaactac ggcttccagt ccggggactc cccggtccgg
     8221 aatcccttcc ggctggatcc ggggacggag gggaccgcgt actggaagga cggctccacc
     8281 ctggcgttca agccctccgc gcccctggcg gccggaggga tctatacggt gtccgtcgac
     8341 ctggcctccg tggacgcggc ggcccgggag cccttctcgt tccggatccg cacccctgaa
     8401 cccgccctga cggtggagtt gggagacctc cgggccgccc gggacgggac cctggaactc
     8461 cccgggcgcg tcgtgctctc cgacgcggaa gcttcggaca aggtggagcg cctcctgtcc
     8521 gcccggttcc gaggcctaaa gcccggaatc gcctggaccc atggcggcgc cgtccacgat
     8581 ttcacggtcc ggggcttctc cgccgcggac cgcgacgcgg acctggagct ttcctgggac
     8641 gggcgccccg tgggcgccgc gtcccgggga gggcgctcgg tacgggtccc cgcggcgggg
     8701 tccttcgagg tcaccggaat ccgggcggtg cgggaggggg agagcttcct ggaagtctcc
     8761 ctgtcctccc ccgtggatcc cggtcaggat ttccggggcc ggatcgaggt ggaaggcatc
     8821 ccggacctgc ggttctcggc cgcgggaggg atcgtccggg tctacgcctc cgcggcctgg
     8881 cccgaggagg cccgggtccg gatcggggag gggctccgtg attccgaggg ccgctccctg
     8941 gcggtgcccg tgcaggcgac ggtgccggtc tcctgggaga agcccgcggt ccgcttcgcc
     9001 acggcgggag tggtcctgcc cacgagcgag gggctcaccc tgcccatcga aacccgcaac
     9061 ctggcggggg tggtggtcga ggtgttccgg atccccgggg acaacatgct ccagttcctc
     9121 caggtgaaca aactggacgg ggacgacgaa ttgtaccgga ccggggaggt ggtctggcgg
     9181 aaggccttcg acctgccctg gagcgacacg cggcgcaacc agtggacggc ggcggggctg
     9241 gacctcggcc ccctcctggc caagtacaag gacgggatgt accgcatccg cctcaccttc
     9301 ctgcgggacc aggtgcggta cgtctgcagg accgagcacg acttcaagga cctggccttc
     9361 ccgaccctgg aaaccctgga ccggcattgg gacggggact gggaggcccg gtacaagttc
     9421 cgggacgacc cctgccatcc cgccttctac gagcgcaatt cgacatacag gaacgtcctg
     9481 atatcggacc tggccctggg ggccaagcgg gacgcggacg gccggaccgg gatcttcgcc
     9541 gtggacctcc gcagcgcccg gccggtcccg gacctggagg tgatcctcta taacttccag
     9601 cgccgggagc tggcccgggg ccggacggac aaggagggct tcgccacctt cccggcctcc
     9661 cgggaggctt tttccgccgt cgccttccgc tccggccagg cggcctacct caagctggac
     9721 cccggctcgg ccctggcggt cagccatttc gacgccggcg ggacggcctc ggagtccggg
     9781 gtcaagggct tcctctacgg cgagcgcggg gtctggcgcc ccggcgacga gatgcacctg
     9841 accttcctcc tcctggaccg cctcaagacc cttcccgcgg gccacccggt ccgcttcgag
     9901 ctggagaacc cgatgggcaa gacggtccag acctcggtac tctccgaggg gaccgacggg
     9961 ttctacccct tcaaggcccg gaccgccgag gatgccccca ccgggaccta cctggcccgg
    10021 gtgaccgtgg gggaccggat cttcacccgg aacgtcaagg tggagaccat cgtcccgaac
    10081 cgcctcaaga tcgagttcga cttcggaggg ggagcctacc tgggcgacgg ggaggcccgg
    10141 ttctccctga aatcccagtg gctccacggc gcacccgcgc cgggcttgaa ggcggacgtg
    10201 tccgtggcct tctccccggc ccccacgacc ttcccgggct tcggggacta cgtcttcgag
    10261 gaccccaccc gccgcctggc cacggagcgg cagacgatct tcgacggccg cctggacacc
    10321 gagggggccg tgtccttcag ttcggccttc gacgccgcgg gcagcgcccc gggggcggcg
    10381 accgcccagt tcgctgtccg ggtcttcgag cccaacggct tcttctccag cgagcagttc
    10441 accgtgccct accaccccta cgcacggtac gtcggggtcc gcctggacac ccggtggggc
    10501 acggtcaagg ccgaccagga ccagacggtc aagctggccc tggtggaccg ggacggaaaa
    10561 cccgtttccg cccagaacct ggaggtgggc atccaccgga tccgggagaa ctggtggtgg
    10621 gagggcggcg aggattccct gcccgaatac gcccggagca tcttcgagcg ctcggtcctg
    10681 aagggccagg cgaccgtccg ggacggccgg ggatcctgga ccttcaaggt caatcaatcc
    10741 gattcctggg gcatctacct gatccgggtc cgggacccgg tctcgggcca ctcggccagc
    10801 caggtcttct gggtcagccg ctacgactgg tacggccggc ccggggagaa agccgcgccc
    10861 tcgaatttgg ccctgacggc ctcccaggag cggtatgtcc cgggacagaa ggtgcgggtc
    10921 tctttcccct ccaactccaa gggtagggcc ctggtgaccg tggagcgctc cggccgggtc
    10981 ctttcccaga agtggcagga ctgcgcggag ggcacgacca ccgtcgagtt caccgcctcc
    11041 gccgagatga gccccaacgc ctacctgcac gtcgacttcg tccagcccca cgcggggcgg
    11101 gggaacgacc tgccgatccg cctctacggg atccttccga tcctggtgga cgaccccgcc
    11161 acggccttgg ctccccggat cgaggcgccc gagagcttcg agccaaacaa gcccgcatcc
    11221 ttccgggtgc gggaagcctc cgggaagccc atgaccgtga ccgtggccgt cgtggacgag
    11281 ggtctcctgg ggatcacccg gtacaagact ccgaaccccc gggacgagtt ctacaagaag
    11341 gaagcctcgg ccctccgggc ctgggacatc ttcgatttcg tcaccggcgc ctactcgggc
    11401 aagctggaaa ccctcctggc cgtgggcggc ggcgacggcg gcgagctgga ggggggccgt
    11461 aagccctccc ggttcccgcc ggtggtgcgg tacttcggcc ccgtgaccct gaaggccggg
    11521 gagacccgga ccttcaccac ggacctggga ccctacgtgg gggcggtccg cgtcatggcc
    11581 gtggccggcc gttccgacgg ggcttacggg gtcgctgaag tcccggtgcc cgtcaaggcc
    11641 cccctcatgg tccaggccac cctcccccgg gtcctctccc tggaggagtg gacctcggtc
    11701 cccgcggcgg tcttctccag cctcgggccc ggagcctccg tcacggtgcg gatttccgcg
    11761 tccggggcgg cggaggtgga gggcgaggcg gcccggacca tcgtcttcga ccgggacggg
    11821 gagcgcaaca ccgagttccg cgtccgggcc ctctcggtgc ccgggaccgc ccggatcgtc
    11881 gtgaccgccg agggaggcgg caagcggtcc gagcacgccg tggacctccc gatccgcccc
    11941 gcgggcgcca aggccgcggc ggactgggag gcccaggctt cccccggcgg cgtagccgag
    12001 ctttccctgg cctacccggg catgccgggc acccacaagg tctggctcga gctatccccc
    12061 ctggagccca tccagctcac ccggcgtgtg aactacctca tcgggtatcc ccacggctac
    12121 gcggagcaga cgatctcaaa ggccttcccc cagatcctcc tgcccgaggc ggccaacctg
    12181 tccgagtggg agctggaaga cgtgcgaacc aacgtgcgcg ccgccatcgc caagctttcc
    12241 gactatcagg ctccctcggg aggcctggcc ctctggcccg ggagcgggcg ggaggacgac
    12301 tgggtctcgg tctacgcggc ccacttcctc ctgaccgccc gggccgcggg ctacgaagtg
    12361 cccgcggggc tcgtcgagcc cctctggaag cacctggacg cccgggccgt ggcctggggc
    12421 tcccagtacc ggtgggccaa ggcgactcag gcctaccgtc tcctggtcct ggcccgggcg
    12481 ggcaagccca accccgcggc catgaaccgt ctgcgggacg cggggaacct cccgacccag
    12541 gccctctggc agctggccgc ggcctacgcc ctttcgggcc agagggggac cgccgcggac
    12601 ctcgtgaagg ggctggaccc ccggatcgag gcggcccggt ccctggagga ggactattac
    12661 tattacgtct acggctccga cttccgggac gccgcggtca tcctggacgc cctgaacgcc
    12721 ctgggggacg aggcccgggc cgcggggctc taccgggaca tcgccaagcg gatgaactcc
    12781 gcctactggt tcagcaccca ggagacggct ttcgccctcc tggccgcctt gccgtattcc
    12841 cggcggatcg ccggggccgg agcctccgcg gcccgctggt cctacaacgg atccccggaa
    12901 gcccggatcg ccttgaccaa gccggtcacc cgggtggccc tgcccgcggg cacggaagcg
    12961 ggcggccgcc tcacggtccg cgccgaggga ggggccccgg tgtacgtccg ggtcctggcg
    13021 gagggggagg cccgggccgg cgaggaggcc cccgaggccc ggggactctc ggtgctcacg
    13081 gtctacaagg acatggaggg ccggaccctg gatcccgaag ccgccccgcc cctgtcggac
    13141 ctggtggtgg agacgacggt gcggaacctc acccgcagga ccgtccgcaa cgtggccctg
    13201 acccagaagc taccctcggg ctgggagatc cggaacttcc gggtcggctc ggaggagcgg
    13261 gagacctggg acgagggcta cgggcagggc tccggccggc cctggtggta cccccgccgg
    13321 gactacacct accaggacgt ccgggacgac cgggtcttca cctacctgga cctcgggccc
    13381 ggcaaggaag cggtggtgcg ggtcttcgcc aacaagacct acgacggccg cttctaccgg
    13441 cccggggcct tcgcggaggc catgtacgac cccgagtacc gcgcgctgga gcggggggcc
    13501 tggatgcggt cctgggagga agcctcctcg ggggcccgga acaagcccac cgtgaacagc
    13561 accctgagat ccaagtgagc cggagggagt ccgcccccgt ggccgcgggg gcggtcctcc
    13621 tggccgccct ggtcctgctg gtccccgggc cgccgaggtt tcccgggggg tattccacgg
    13681 tcctctacga ccggaacggc ctgctcctgg gggccgcggc cgcgccggac gggcagtggc
    13741 gcttcccccc ggccccggac ctgccggacc gctacgtccg ggccctggtc gccttcgagg
    13801 accgccgctt cttcgggcac gccggcttcg atccccgggc cctggcccgg gccctggtct
    13861 ccaacctggc cgcgggccgg atcgtgtccg gcggctccac cctcagcatg caagccgccc
    13921 ggatggcccg ggggaaccgt ccccggaacc tggggcagaa ggccctggag ctgttgtacg
    13981 cccttcgtct ggaagccttc cggggcaagc gggggatcct ggccctctac gcggcccacg
    14041 cccccttcgg aggcaacgtg gtgggcctgg ccgcggccag ctggcgctac ttcggacggc
    14101 ccccggaaga tctcacctgg gcggaggcgg cggtcctggc ggtcctgccc aactcccccg
    14161 ccctggtcca ccccggggcg gaccgggacc ggctccggga aaagcgggac gccctcctta
    14221 tgaggctggc cgagcggggc gagctcgggc ccgaggacct ggcggccgcc cgggcggagc
    14281 ccatcccgga ccgtcccgag cccctgcccc ggaaggcccc ccatctcctt tcccgggcgg
    14341 tacgggacgg cctggccggg acccgggtgg acagcaccct ggacgggggg ctccaggacc
    14401 tcgtggcccg gaccgcggcc cggcacgggg cgcggctggc gtcccggggg atccggaacg
    14461 ccgcggtgat cgtggcggag atcgagagcg gggagatctt ggcctacctg gggaacctgg
    14521 acggtccgga gggcggggag gaccgggagg cggcccgctg ggtggactgc gccgccgctc
    14581 cccgcagcac cgggagcatc ctgaagccct tcctctacgc ggccctcctg gacggggggg
    14641 atctgagccc gacgaccctg gtgcccgacc tgcccaccag cttcgggtcc ttctccccgg
    14701 agaacaacac ccggacctat tccggggcgg ttcccgcgga gcgggccctg gcccactccc
    14761 tgaacgtccc cttcgtgcgc ctcctccgga cctacggggt ggacgccttc ctggccctcc
    14821 tcaagggcta cgggttctcc gagctgggca ggaccgcggg ggcctacggc ctgaccctgg
    14881 tcctgggcgg ggccgaggcc accctgtggg actccgcctc ggcctacgcg gacctggccc
    14941 ggtccgcccg gggactggaa ggcgttccgg ctttcgccta cagccggact cctccggcgc
    15001 cgcggggctc cggctccgtc cccgcctccc ccggggcggc ctacctgaca ctgcgagccc
    15061 tcctggaggt cggacgcccc gcggaggagt cggcctggaa ggactgggcc gagggccggc
    15121 gcatcgcctg gaagaccgga accagccagg gcttccggga cgcctgggcc ctgggggtca
    15181 cctccgagca cctggtggcg gtctgggtcg ggaacgcgac gggggagggg aggcccgagc
    15241 tccggggctc cgaggccgcg gcccccctgt tgttcgacgt cttcgagctt ttaccccggg
    15301 acccctggat cgaccctccg ccggacgccc tgcgccggga gacggtctgc gcggactccg
    15361 gctacctggc gggccccgac tgcgcccgga ccctccggat cgacctgccc tcgggggccc
    15421 ctcctccgga tccctgtccc tactgccgga cgatccacct gaccgcggac ggcgcgttcc
    15481 gggcgaccgc ggactgcgtg cccctggacc gcctggtctc ccggaaggtc ttcgtcctcc
    15541 cccccgccct ggagtggtac ttccgggccg gacacccggc ctaccggccc ctgccgcccg
    15601 tcctgccggg ctgcgggacc gtggaggaca tccccgtcac ggtcctggtc cccgaggagg
    15661 gcgcccggct cttcatcccc gtggagctgg acgggactcc cggccgcgcc gtattccggg
    15721 cggtgcaccg ggatccccgg gcccggctct tctggcacct ggacggagac tacctggggg
    15781 aaaccacagg ccccgagcac aggctggagg tacgcccggc ccggggacgg agcgtcctga
    15841 ccgtggtgga ccaggagggc cggacctcga cccgccgctt cgaggtcctg agcgaacggg
    15901 aaggctccta agctccgccg attccggata tactcaggcc catgaaagcc ttcccgcccc
    15961 ggacccgccg gtccgccttc gccgtcctct gggctctgtc cctcctgcga cttgccgccc
    16021 aggacgcttc ctggctcggc ttctgggagg gggagttccg gaccgcccag ggggattttc
    16081 cggcccggct ggagatcttt tccgggggcg ccctcctgga cctgcccgaa cagggggcct
    16141 tcggctggcc cgtccggacc ctgaaggccg agggctccga tctctacctt tccgtggaac
    16201 tggggatgga acccctggaa ctcgcgggtc cccgggaggg ggaggtcgtc gcgggaaccc
    16261 tcatccaggg ggggcggtcc gggcgtttcc agctgcgccg ggcgccggac gcccgggacc
    16321 gggacgggga gcggtctttc gacaccggcc ggggggtcct tccgggcacc ctgcgccttc
    16381 cccccggcgc ggccggcccc ctaaccctgg cgatcctcct gcccgattcg ggtaccgccg
    16441 accgggacgg caacaactgg caggtccccg gccgcaacga ctccctccgg atgctcgcgg
    16501 agtccctggc cgaagccggc atcgccagct accggtacga caggcgcggg gccggaaagg
    16561 cctaccgcct gggggaggac gaggccgggc tcgtattcga ggaccatgtg cgggacgcgg
    16621 ccgcctgcct ccgctccttc gaaggcgacc cgcgcttcgg cagggtcctc gtcttcggcc
    16681 tgggggacgg ggccctggtc ggggcggcgg ccctccggga ggcgaaggcg gatgccctgg
    16741 ccgccctggg ggtctcgggc cggagcctcc gggacataat cctggaagcc gccctgcggg
    16801 cccccgagga gtacagggcc gagatcgtgc gggtcctggg ggagctggac gcggggcgct
    16861 tcgtggacgc ggtcagcccc ttcctggaaa acctcttccg caggagcttc cagccctacc
    16921 tggcctc
//
LOCUS       DAOTEI010000068        16513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2379_length_16513_cov_5.324584, whole genome shotgun sequence.
ACCESSION   DAOTEI010000068 DAOTEI010000000
VERSION     DAOTEI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 16513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16513
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2379_length_16513_cov_5.324584"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="P5313_08200"
     CDS             <1..201
                     /locus_tag="P5313_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80383.1"
                     /translation="PEGTPEERRDKAVGLYVDPVAAQAGLKPVAKSAFGGRMAFFGKV
                     MIDNWEAEPVAAWARELARALR"
     gene            complement(248..1705)
                     /locus_tag="P5313_08205"
     CDS             complement(248..1705)
                     /locus_tag="P5313_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY80384.1"
                     /translation="MKTEAKTARPEKANPASPGTPPEAAGATSSLREALYRSLLENSI
                     DAVYLLSEEGRVLDVNRVACAMTGYTREELLGLTIDDLDPNYPSRGFVEFWNVRPEGA
                     TVLFETLHRRRDGTLVPVEVNGIFTKVEGRKFLFGVARDISERKRAERSLRESETKYR
                     TLVDVLPVGVTITDASGKILETNRMSERILGLSREDHAGRRIDGTEWRIVRADGSPMP
                     PEEYASTRALREGRLVEGVEMGILKEPGETTWLTVNSAPLPLEGYGVAVAYSDVSAPR
                     RSEEHVRSLLREKEILLRETHHRVKNNLQTILSLLALQMDEVSDPESLARLRDLSGRV
                     RSMLVLYNKLYDSGDFGRLDLREYLGPLVREILGIFPPTPPVRLETEVDGVVLDARTL
                     SSLGIILNEWTTNSMKYAFAGLPDGVIRISARREGPRVVCAYEDNGPGIPEEAVGPGN
                     GGFGMRLIGLLAEQMGGDCRREGARWTLSFPADDR"
     gene            1870..2838
                     /locus_tag="P5313_08210"
     CDS             1870..2838
                     /locus_tag="P5313_08210"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HRY80385.1"
                     /translation="MPLFRPELFQGARRRRRYFEGWYFKQVSPDGRAWSFIPGVSLDA
                     EGRRRAFVQAIDGSAGTTRWYEFPFEAFRWDRRSLDLRVGGNRFSLSGIGLDLDGPEG
                     RIRAELRFGPSVRFPSRPWRPGIMGPYSFVPFLECNHGLVSLDHRTDGRVEADGVGIS
                     MDGGRGYIEKDWGSSMPAAWIWCQSNSFPEPGVSLLFSLADIPWLGGRFPGFLCAALL
                     PGEGVRVWASWNGSRIDRLEVGDRSIGVRIRRRDEILDLRMERGRGGLLRAPAAGAMD
                     RRIAESVDAVLWARLEGRGRRIFEGTARPAGLETVGDHSLLAPGGS"
     gene            complement(2977..4545)
                     /locus_tag="P5313_08215"
     CDS             complement(2977..4545)
                     /locus_tag="P5313_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY80386.1"
                     /translation="MKTPETSPPRIRRVLLAEPESAGETSPAPALEGFGYEVVVERSE
                     SGAEARLRSDPLIGLALLDVDLDGDGACARLAASIPEFRDLPVVFLDAKPGNAASERV
                     RSVPRYGYVSRNAAPFILRSVLETAFDLFEARNRLSERNRILDKINDYALRLDGIPLE
                     SIYRFALENAVDIFKARISAANSYDEAGRVLILEDFASSEDESRTLIGAVGRRMLGMR
                     TPVSEANYRRMVIERVGVVSSIHEMSFGKVPGTVSNILQQSLRADWFRGVALLSEGQL
                     YGTLILVGQESRPPPEPDELLAFAEITASTLKRKAAEQRIRHLLSEKDLLLHEVHHRI
                     KNNMATVIGLLDLQSESVADEAAAEALREAKSRLQSMGTLYEKLYRTENLREMPLGEY
                     IPDLAAEIVGMFPKGGFVRIETRIEEFNLPVKTVSVLGIILNELLTNAMKYAFRDREE
                     GHIRITAARKGRRVAVEVRDDGAGLPVSLDPFKTSSFGLRLVRVLTDQLGGSVRFESK
                     GGTAVFLDLPYPGR"
     gene            complement(4511..4690)
                     /locus_tag="P5313_08220"
     CDS             complement(4511..4690)
                     /locus_tag="P5313_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80387.1"
                     /translation="MKPNYTGEKRRKELERKKKKEEKELKKKLRREAAARGELPEGEE
                     PESDDEDSGDESAED"
     gene            complement(4785..5897)
                     /locus_tag="P5313_08225"
     CDS             complement(4785..5897)
                     /locus_tag="P5313_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY80388.1"
                     /translation="MPAPKKLLLVEDEAVIALAEARVLGQSGYDVEIVHTGEASVEAI
                     RSDPAVDLVLMDIDLGPGLSGPDAAARILELRSLPIVFLTSHSEREMVDKVRNITRYG
                     YVVKNSGGFVLLSSIELAFELFSAHEEVRRQSRRREAMLQAIPDLIFSVDRDGTIRDF
                     HAPPGVGLAVPEERVAGTNIREILPPGEAERHLALYRTCLETGEVLTDSYSLNPDGRI
                     RIYELRLARQDENQVLAVVRNVTDRIESERQLRRVTAHYEYLFDCAPVPIVLLDGEDR
                     VVKVNANFLRTFGYSQEEAVGRPINDLVVPPEHGEEGRDYSRRVASGEPLQCEAVRRR
                     KDGSRIRVSITASLIELEGSFYIYAIYQPVADWAAP"
     gene            6011..6943
                     /locus_tag="P5313_08230"
     CDS             6011..6943
                     /locus_tag="P5313_08230"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRY80389.1"
                     /translation="MRSTMKAAISPRRCRNPGPALILAAGVSLGSCATIPASVADLPR
                     NPEGSVVLAAGVDYREIRRDLPRLLRIHVLRADLAAGNLAIRVPLGPDPDGPGPAEAR
                     LEDPLRLARASRALVLVNASSFGVLGYPEGERPPVYLRGMGVDIAGLAADSGRVASPS
                     NPRYASFYVDGRGVPRIAGHGEVPERDVAVREAAAGFSPLVVRGEVAEERGGDIEPRT
                     AVGLDGRGRLILVVAEGRRLGRSEGLTLGELAELMYELGCVDALNLDGGGSSVLVVRV
                     LGWGYRVLAPPTDGAGPLRFRRPVPNALAVVAGE"
     gene            complement(7023..7607)
                     /locus_tag="P5313_08235"
     CDS             complement(7023..7607)
                     /locus_tag="P5313_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRY80390.1"
                     /translation="MGDAAVNLSERYTYGDYRTWPDDRRWELIDGAAWAMSPGPNRRH
                     QGILVRLSNAFFQFLRGKPYAMYIAPFDVLLPIGNEDDDSVETVVQPDLVVYCDRSRL
                     RDYGARGAPDLVAEILSPGTSKRDLNDKYRLYESRGVREYWVVDPGNRALTVFRLGPS
                     GAFGPGEVHVNTGGAASSVLEGLAVDLEDLFRED"
     gene            complement(7705..8646)
                     /locus_tag="P5313_08240"
     CDS             complement(7705..8646)
                     /locus_tag="P5313_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRY80391.1"
                     /translation="MNETPGLLLALGAGLLSFLSPCILPLIPGYISFISGSGAADAAG
                     GGTPRRAVLARTAAFALGFTAVFTALGLVFGGGALLLGGAARRITAAAGIVIVLFGLN
                     TLFDFLKFLNAERRAHPTARPSGLGGAFVVGLAFGAGWTPCVGPILASILLFAGRSGN
                     PWAAGGLLAVYSLGLALPFLAAGAFLDRMKPLLDWFKRRGREVRIASGLMLVLLGLGM
                     ALGQIEALNGSFARAGFALQSFAGSNPGGARAAALVAYGALAAIAILIPRARGKSFLR
                     PGRLAFLALLAAAALGEILGWWSGVSTLSGWLLFQGI"
     gene            complement(8643..9290)
                     /locus_tag="P5313_08245"
     CDS             complement(8643..9290)
                     /locus_tag="P5313_08245"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY80392.1"
                     /translation="MKYVFPILIAVAVLAGGCSRAERTGTASSSDSPAGAVPSGAPAA
                     APVSASVPETPSSRPWYADRFEALGFYVFPAPVELPSFSVQPRAGGPALVPAALKGKV
                     TLLNFWATWCPPCREEMPSIEKLHSALRGEAFAVAAISVKEDAKTVDGFLKQYPYTFP
                     IYLDPTGAASGNFVTRGIPTTFVLDKDARAIAAIIGSRPYDEPEVISLFRDLARR"
     gene            9445..10065
                     /locus_tag="P5313_08250"
     CDS             9445..10065
                     /locus_tag="P5313_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRY80393.1"
                     /translation="MKSLRSLRDILRAEGEEEDTAPEKIAFPDPLAERFLKTRTILLS
                     GEVNKETAEKVISRLLLLESQSDDPIRVLIDSPGGDVDAGYAIFDMARFVKPQVWMIG
                     MGLVASAGALILLAAPKERRVGLPNSHYLIHQPLSGLRGVATDIEIHAREIERTRDRI
                     NKLIAQETGQDLERVQKDTDRDYWMTAEEAVAYGLISRTVNRRDEL"
     gene            10069..11046
                     /locus_tag="P5313_08255"
     CDS             10069..11046
                     /locus_tag="P5313_08255"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRY80394.1"
                     /translation="MDRARIEAALAGAYPGLPDAAAGGRPVYRGKVRDVVEGPGRLLL
                     VASDRISAFDRVLSTIPFKGEVLTRVSAWWFARTSDIVPNHLVPPSHLGGRDPVRETG
                     RACLVRRAEMVPVEVVVRGFLTGSAWRDYQAGRTVSGIALPSGLARDSEFPEPLLTPS
                     TKEASGHDRPASGEELVRSGRVDAELWEEISRAARALYLRGREVAREHGLVLVDTKYE
                     FGVADGRLVLGDEIHTPDSSRYWRLPGPGASAPGARSELDKESFRRWLAERGFTGDGE
                     APSVTDEIRSETAARYVEAFEAITGEVFEPNPLPTQALDELLAEWASTV"
     gene            11113..12171
                     /locus_tag="P5313_08260"
     CDS             11113..12171
                     /locus_tag="P5313_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY80395.1"
                     /translation="MRIPRLGAAAAAALAVLSLCSSRCSVLDLPRGEISVLSWNLQTL
                     FDDRDDGVEFPGWSVANGQWDSEGYRTRLRNFSRAASAAVPGGPDILVLQEVENQRVA
                     EDLAALLPGSWPTLIVSGGEGAAFGCAVLSRFPAVVARAHAVDPGPGFRPEDLRPLLE
                     VVLDAEGIRLTILAAHWKSKVGGAEATEPARRAQAALAARRIAEILAEDPAAELLLAG
                     DLNENPDEWERVGRAWPTALLPADRAARADGWKDRILLAADPGNTGPGPDGLVLWSPW
                     EEAGGFSYVHEGTEERIDHVLLCPGLLDGKGLAFSSFSAVTERELLSEDGTPRAWDTR
                     NREGYSDHLPLRLALAWR"
     gene            12323..13996
                     /locus_tag="P5313_08265"
     CDS             12323..13996
                     /locus_tag="P5313_08265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY80396.1"
                     /translation="MSDPKPLIVQSDLSLLLDAHDPGFEKAREDLGPFAQLEKSPEHF
                     HSYRITPLSLWNAAAAGFTAEDAAAVLTRWARYEVPQSVLYTIREIASRFGKLLLREG
                     DAAGELLLTCEDKRVAAEISSSRRLAKWLSPAPGGFRVRLTDRGTVKRELTALGWPVK
                     DLAPLAEGEPLAFALRETTGAGRPFSLRPYQTDAVRAFLGDKGPGTGYGVVVLPCGAG
                     KTVVAMAAAAQIGRKTLVVTTNVAAVHQWMDELADKTDLAPGDVGEYTGARKEVRPVT
                     IATYQVLVWRPDKESDYPHFDLFRREKWGLVIYDEVHLLPAPVFRVVAEIQAVRRLGL
                     TATLVREDGREEDVFSLIGPKKYDVPWKDLERKGFIAEAFCREIRVALPEDEAVRYAV
                     AETRGKHRIAAENPRKLEIVRELVANHPEDSILVIGQYLDQLGAIARDLGAPLITGAV
                     PNAERERIYGDFKAGRVRVIVVSKVANFAIDLPDASVAVQVSGAFGSRQEEAQRLGRI
                     LRPKERNSWFYSLVSRFTGEEDFAANRHRFLAEQGYRYTIESWDVDPWN"
     gene            13987..16080
                     /locus_tag="P5313_08270"
     CDS             13987..16080
                     /locus_tag="P5313_08270"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HRY80397.1"
                     /translation="MELKTLPAPVWRDALVTEDTEAFLSAVRNYLGPVPTPYNKHSLV
                     ARLEAFLKKPQTAEAQAALLDSLDALILGALAAAGPCPESFLRELFADERGPWEISLR
                     LANLRDRLVLFRAPGPAGPVLAVTPPLASRLAREADLRGLLGCAPSEGPPPPPRVDAD
                     TICAAASILFHEENPLRRDGRPAKRVRARLLALLPELGGASEPAGADEILDALLAGFR
                     GAGFLEGPEGTPDMDRLAAAAEAWGDRLPFLVAAGSRELDFRPDAEAGPSPRPSLAES
                     ARALESIAAALPTGIAVSRAGFRRLALLSAEPRGSAGAGRAETSAEALLRFGLVERTA
                     SGIVRAAASRRVRSTIPEAGGIPSGPVLAVEASHALRVLPEADPAARVFAGTVARLES
                     RGELWSCVLDRKSVRRALDAGLGSERIARTLERLSGRPLPQSLAYSLTGWEKEHLSVR
                     LYYGYVLAAEGPARTRAENAPALAGLRGPAVAEGAWLLESEDPGEIRTALEKAGLHVP
                     RTRRSGRPEPSGTNASGSGAAGLLAPVTQAERPAWSRLLDGPVDRAGEPGPARSPDSL
                     ARLRARLGELDLPEGRRAELAERIGRRIILTEDQLIRAETDGPGLEAGALDYLGKVRV
                     VERVLRSGGALLEILTRSPDGRPELFRVRPLELEKTDRGLVLRAADAEDGSVRILPVR
                     SMSHVKRIKTTLFGD"
     gene            16082..>16513
                     /locus_tag="P5313_08275"
     CDS             16082..>16513
                     /locus_tag="P5313_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY80398.1"
                     /translation="MTTMPNKNNDTMQLKLAVLIDADNTQAWIVDGLLAEVAKYGIAS
                     VKRIYGDWTTTNLRHWKDKLLAYAIQPIQQFAYTSGKNATDSAMIIDAMDLLYSERLD
                     GFCVVTSDSDFTRLAARLRESGKLVLGFGEKKTPKPFVAACD"
BASE COUNT         2417 a         5697 c         5846 g         2553 t
ORIGIN      
        1 cccgagggca ccccggagga gcgccgggac aaggcggtgg gcctctacgt cgatcccgtg
       61 gccgcccagg cggggctaaa acccgtggcc aagtccgcct tcgggggccg catggccttc
      121 ttcggcaagg tgatgatcga caactgggag gccgagcccg tggccgcctg ggcccgggag
      181 ctcgcgcgcg cgctccgcta ggttccgccg tcgacgggac ggggtctacg gccctcggcg
      241 gccgggccta ccggtcgtcc gccgggaagc tcagggtcca ccgggcccct tcccggcggc
      301 agtcccctcc catctgctcc gccaacaatc cgatcagccg catcccgaag ccgccgttcc
      361 cggggccgac ggcctcctcg gggatgccgg gaccgttgtc ctcgtaggcg cagaccacgc
      421 gcgggccttc ccgtcgggcg gagatccgta tgaccccgtc cgggagcccc gcgaaggcgt
      481 acttcatgga gttcgtggtc cattcgttca ggatgatccc cagggaggac agggtccggg
      541 cgtccaggac gacgccgtcg acctcggtct ccaggcggac cgggggtgtg ggcgggaaga
      601 tccccaggat ctcccgtacc agggggccca ggtactcccg gaggtccagt cggccgaagt
      661 ctccggagtc gtagagcttg ttgtacaata cgagcatgga ccgcacccgg cccgagaggt
      721 cccggagccg ggccagggac tccggatcgg agacctcgtc catctggagg gcgagaaggc
      781 tcaggatggt ctggaggttg ttcttgaccc ggtggtgggt ttccctgagg aggatctcct
      841 tctcccggag cagggaccgg acgtgctcct ccgagcgccg gggcgcgctg acgtcgctgt
      901 aggccaccgc caccccgtag ccctccaggg ggaggggcgc ggagttgacg gtcagccagg
      961 tggtctcccc gggctccttc aggatcccca tctccacgcc ctccacgagg cgcccctccc
     1021 ggagggcccg ggtgctggcg tactcctccg ggggcatggg gctgccgtcg gcccggacga
     1081 tccgccactc ggtcccgtcg atccgtcttc cggcgtggtc ctcccgggac aggccgagga
     1141 tgcgctcgga catgcggttg gtctccagga tcttcccgga ggcgtcggtg atcgtgacgc
     1201 ccacgggcag gacatccacc agggtccggt acttggtctc gctctcccgc agggaacgct
     1261 ccgcccgctt ccgctcggag atgtcccggg ccacgccgaa caggaatttt cggccctcca
     1321 ccttggtgaa gatcccgttc acctcgacgg ggaccagggt cccgtccctg cggcggtgga
     1381 gcgtctcgaa gaggacggtg gccccctcgg gccggacgtt ccagaattcc acgaaccccc
     1441 tggacgggta gttcggatcc aggtcgtcga tcgtgaggcc gaggagttcc tcccgggtgt
     1501 acccggtcat ggcgcaggcc acgcggttca cgtccaggac ccggccttcc tcgctgagca
     1561 ggtatacggc gtcgatgctg ttttccagaa gactccggta cagggcctcc cgcagggagg
     1621 acgtcgcgcc ggccgcctcc ggcggggttc cgggagaggc gggattcgct ttttctggcc
     1681 gcgccgtttt tgcctcggtt ttcatcctgg agcctccttc ccggcagggc gcccgtcggg
     1741 actccgggtt cgagtgtacg cccgtttccc gggggcgtca aacccgcgac gcccggcttc
     1801 cggatatccc tcaagtggat tatcctgaga aagcagggcc gcggcctcgc ggcgcccgaa
     1861 aggagcccca tgcccctgtt ccgtcccgag ctcttccagg gcgcccgcag gcggcgccgc
     1921 tacttcgagg gctggtactt caagcaggtg tccccggacg gaagggcctg gtccttcatc
     1981 cccggggtga gcctggacgc ggaggggcgg cgcagggcct tcgtccaggc catagacggg
     2041 agcgccggga ccacccggtg gtacgagttc cccttcgagg ccttccgctg ggaccgccga
     2101 tccctggacc tccgggtggg gggcaaccgt ttctccctgt ccgggatcgg gctggacctg
     2161 gacggaccgg aggggcggat ccgcgccgag ctccgcttcg gtccgtccgt ccggttcccc
     2221 tcccgcccct ggaggccggg gatcatgggc ccctactcct tcgtgccctt cctggagtgc
     2281 aaccacgggc tcgtgtccct ggaccaccgg acggacgggc gggtggaggc cgacggggtc
     2341 gggatctcca tggacggcgg ccggggctac atagagaagg actggggctc ctccatgccc
     2401 gccgcctgga tctggtgcca gtcgaactcc ttcccggaac cgggggtgtc cctcctgttc
     2461 tccctggcgg acattccctg gctgggcggg cgcttcccgg gcttcctctg cgcggccctc
     2521 ctgcccgggg agggggtccg tgtctgggcg tcctggaacg gctcccggat cgaccgcctg
     2581 gaggtcgggg accggagcat cggcgtccgg atccggcggc gggacgagat cctggatctg
     2641 aggatggagc ggggccgggg aggcctgctc cgggcccccg cggcgggggc catggaccgc
     2701 aggatcgcgg aatccgtgga cgcggtgctc tgggcgcggc tggaaggccg gggccgccgg
     2761 atcttcgagg ggaccgcccg gcccgcgggc ctcgagacgg tgggggatca ctcgctgctc
     2821 gcgcccgggg ggtcctagga gcctatcgac ttgtggatct ttagcgccga taggcgctaa
     2881 agatcccgat tatccggctc ctttggagtt tgtaaagtag aaaaatcgca caagtgcgat
     2941 ttttctactt ttaggtgggg caagcccgac aaactcctac cggcccggat agggcagatc
     3001 caggaacacg gccgtcccgc ccttgctctc gaaccggacg cttcctccca gctggtccgt
     3061 caggaccctc accaggcgga gcccgaagct ggaggtctta aaggggtcca gggaaaccgg
     3121 caggcccgcc ccgtcgtccc gtacctcgac ggcgacccgt cgtcccttcc gggcggccgt
     3181 gatccggata tgcccctcct cccggtcccg gaaggcgtac ttcatggcgt tcgtgagcag
     3241 ctcgttcagg atgatcccca ggacggagac ggtcttcacg ggcaggttga actcctcgat
     3301 ccgggtctcg atccggacga acccgccctt cgggaacatc cccacgatct ccgccgccag
     3361 gtccgggatg tactcgccca ggggcatctc ccgcaggttt tccgtccggt agagtttctc
     3421 gtagagggtc cccatgctct gcagtcggga cttggcttcc cgcagggctt ccgcggccgc
     3481 ctcgtccgcg acggactcgg attgaaggtc cagaaggccg atcaccgtgg ccatgttgtt
     3541 cttgatgcgg tggtggacct cgtgcaggag gaggtccttc tccgagagca ggtgccgaat
     3601 cctctgctcc gccgccttcc gtttgagggt gctcgccgtg atttccgcga aggccaggag
     3661 ttcgtcgggt tccggcggag gccgcgattc ctgtccgacc aggatcagtg tcccgtacaa
     3721 ctggccctcg gagagcaggg cgactcctcg gaaccaatcc gcccgaaggg attgttgcag
     3781 gatgttgctc acggttccgg gaaccttgcc gaaggacatc tcgtggatgc tcgaaaccac
     3841 tccgacccgc tctatgacca tccttcgata attcgcttcg ctcaccggtg tgcgcattcc
     3901 cagcatcctg cggcccacgg ccccgatgag cgtccggctc tcgtcctcgg agcttgcgaa
     3961 atcctccaag atcaagactc tgcccgcctc atcgtagctg ttcgcggcgc tgatcctcgc
     4021 cttgaatatg tccacggcgt tttccagcgc gaatcggtag atcgattcca gggggatccc
     4081 gtccagtcga agggcatagt cgttgatctt gtccaggatt cggttccgct ccgacaggcg
     4141 gttacgggct tcgaagaggt cgaaggcggt ctccaggacc gagcggagga tgaagggggc
     4201 ggcgttcctg ctgacgtagc cgtatcgggg aacgctccgc acgcgctccg aggcggcgtt
     4261 tccgggcttg gcgtccagga agacgacggg aaggtcccgg aactcgggga tcgaggccgc
     4321 cagtcgggcg caggctccgt ccccgtccag gtcgacgtcc aggagggcca ggccgatgag
     4381 gggatccgag cgcagccggg cttccgcccc ggattccgag cgctcgacga ccacctcgta
     4441 gccgaagcct tcgagggccg gagccggcga ggtctcgccc gcggattccg gctccgcgag
     4501 aaggacgcgc ctaatcctcg gcggactcgt ctccggagtc ttcatcgtcc gattccggct
     4561 cctccccctc ggggagctcg ccgcgcgccg cggcttccct gcgcagcttc ttcttgagtt
     4621 ccttctcttc cttcttcttc ttgcgctcca gttccttccg gcgcttctct cccgtgtagt
     4681 taggtttcaa tccggtttcc cgctcctcgg tatgggtacc tacactatag tccaatacgc
     4741 cgccgattgt aagacgcggg gaggagccgc ggccgccccg gggttcaagg cgcggcccaa
     4801 tcggcgacgg gctggtagat ggcgtagatg tagaaactcc cttcgagttc gatcaaggac
     4861 gccgtgatgg atacgcggat gcgggagccg tccttccttc gtcggaccgc ctcgcactgc
     4921 aggggttcgc cggacgccac acgcctggag tagtcccggc cctcctcccc gtgttccgga
     4981 ggcaccacca ggtcgttgat ggggcggccg acggcctcct cctggctgta gccgaaggtc
     5041 cggaggaagt tcgcgttgac cttgaccacc cggtcctcgc cgtccaggag gacgatgggc
     5101 accggcgcgc agtcgaagag gtattcgtag tgggcggtga cccggcggag ctgccgttcg
     5161 gattcgatcc tgtcggtgac gttccggacc accgccagga cctggttctc gtcctgccgg
     5221 gccagccgca gctcgtagat ccggatccgg ccgtcgggat tcagggaata cgaatccgtg
     5281 aggacctccc cggtctccag gcaggtccgg tagagggcca ggtggcgttc cgcctccccg
     5341 gggggcagga tctcccggat gttcgtgccc gccacgcgtt cctcggggac cgccaagccg
     5401 acgcccgggg gagcgtggaa gtcccggatg gtcccgtccc ggtcgacgga aaagatgagg
     5461 tccgggatcg cctggagcat ggcctccctg cgccgggact gccgccgcac ctcttcgtgg
     5521 gcgctgaaga gctcgaaggc cagctcgatg gaggacagga ggacgaagcc gcccgagttc
     5581 ttgacgacgt acccgtaccg ggtgatgttc cggaccttgt cgaccatctc ccgctcggaa
     5641 tgggaggtca ggaagacgat gggcagggac cgcagctcca ggatccgggc cgccgcgtcg
     5701 gggccgctca gtccggggcc caggtcgatg tccatcagga ccaggtcgac cgcgggatcg
     5761 gagcggatcg cctcgacgct cgcctccccg gtatgcacga tctccacgtc atagcccgac
     5821 tgccccagta cccgggcttc cgccagggct atgacggctt cgtcttccac gaggaggagc
     5881 ttcttggggg cgggcatggg cgcaacctcc gttggccggc ctcggcgttc tcggggatcg
     5941 ggggacgctc cctgactatc ctggattccc gggcctttgg caagggatgg aatcccattt
     6001 atactccggg atgaggtcga cgatgaaggc tgcgatttct ccgagacggt gccgcaaccc
     6061 gggccccgcg ctgatcctgg ccgcgggtgt ctccctgggt tcctgcgcca cgattcccgc
     6121 gtccgtagcg gacctgcccc gaaatccgga gggatcggtc gtactcgccg cgggcgtcga
     6181 ctaccgtgaa atccggcgcg accttccgcg cctcctccgg atccacgtcc ttcgggccga
     6241 cctcgcggcc gggaacctgg cgatccgggt gcccctggga ccggacccgg acggtcccgg
     6301 tcccgcggag gcccgcctcg aggatcccct ccgcttggcg cgggcttccc gggccctggt
     6361 cctggtcaac gcctcgtcct tcggggtcct cgggtatccg gagggagagc gtcccccggt
     6421 ctatctccgg ggcatgggcg tcgacatcgc gggccttgcg gcggactcgg gccgggtcgc
     6481 cagcccctcg aatccccggt acgcttcgtt ctacgtggac ggccggggcg ttccgcggat
     6541 cgcggggcac ggggaggttc cggagcggga cgtcgcggtc cgggaagcgg ccgcggggtt
     6601 ctcccccctc gtcgtccggg gtgaggtagc cgaggagagg ggcggggata tcgagccgag
     6661 gacggcggtc ggcctggacg gccggggccg cctgatcctg gtcgtggccg agggccgccg
     6721 actcggccga agcgagggcc tgaccctggg ggaactggcg gagctgatgt acgagctggg
     6781 ctgcgtcgac gccctcaacc tggacggggg aggctccagc gtcctcgtgg tccgggttct
     6841 cggctgggga taccgggtcc tggcgccccc cacggacggg gcgggtcccc tgcggttccg
     6901 cagaccagta ccgaacgccc tggcggtggt cgccggggag tagggagccg agtcggacac
     6961 ggctcccggg gaggcgccgg cccgcgacgc aggagccgcg ggcataggag ggaccgggta
     7021 cttcaatcct cccggaagag gtcctccagg tccacggcca ggccctcgag cacggagctc
     7081 gccgcgccgc cggtgttcac gtggacctcc cccggcccga aggcgccgga gggcccgagc
     7141 cggaagaccg tgagcgcccg gttgcccgga tcgaccaccc agtactcccg gaccccccgg
     7201 gactcgtaga gtcggtactt gtcgttcaga tcccgcttgc tcgtgccggg ggagaggatc
     7261 tccgccacca ggtccggggc acctcgggcg ccgtagtccc tcaggcggga gcgatcgcag
     7321 tacaccacca ggtccggctg gaccacggtc tccacggagt cgtcgtcctc gttcccgatg
     7381 ggaaggagga cgtcgaaggg ggcgatgtac atcgcgtagg gttttccccg caggaattgg
     7441 aagaatgcat tcgagagcct gaccagaatc ccctggtgcc tccggttcgg gccggggctc
     7501 atcgcccagg cggccccgtc gatcagctcc cagcgccggt cgtccggcca ggtccggtag
     7561 tccccgtacg tgtagcgctc cgaaaggttc accgcggcgt ctcccatggg tacacctcca
     7621 gtatacggca taccgacggg aaaggggaat ttcgcttgcc ccccgggtcg ggcgccggcc
     7681 ggccgtgtat tctgcggagc ttccctatat tccctggaag aggagccacc ccgacagggt
     7741 ggagaccccc gaccaccagc ccaggatctc cccgagggcc gcggcggcca ggagggccag
     7801 gaaggcgagg cggccgggcc gaaggaagga tttccctcga gcccgcggga tcaggatcgc
     7861 gatggcggcc agcgccccgt aggcgaccag ggcggccgcc cgggcgccgc cggggttcga
     7921 gcccgcgaag gattggaggg cgaatcccgc ccgggcgaag gaaccgttca gggcctcgat
     7981 ctgtcccagg gccatgccca ggcccaggag gaccagcata aggcccgagg cgatccggac
     8041 ttcccggccc cggcgcttga accagtccag gaggggtttc atccggtcca ggaaggcgcc
     8101 ggctgccagg aagggcaggg ccaggcccag ggaatagacc gccaggaggc cgcccgcggc
     8161 ccaggggttt ccgctacgcc ccgcgaacag caggatggag gccaggatgg gacccacgca
     8221 gggggtccag cccgcgccga aggcgagccc caccacgaag gccccgccca gtcccgaggg
     8281 ccgggcggtc gggtgggccc gtctctcggc gttcaggaac ttcaggaagt cgaagagggt
     8341 gttgaggccg aacaggacga tcacgatccc cgcggcggcg gtgatcctcc gggcagctcc
     8401 gcccaggagg agggcccccc cgccgaagac caagcccagg gcggtgaaga ccgccgtgaa
     8461 gcctagggcg aaggccgcgg tccgggccag gaccgcccgg cgcggggtcc cgcccccggc
     8521 ggcgtccgcg gccccggagc cggagatgaa ggagatgtag ccgggaatca ggggcaggat
     8581 gcagggggac aggaaggaca aaaggcccgc acccagggcc aggaggagtc cgggggtctc
     8641 gttcatcgac gggccaggtc ccggaagagg gagatcacct ccggctcgtc gtagggccgg
     8701 gagccgatga tggccgcgat cgcccgggcg tccttgtcca ggacgaaggt ggtcgggatg
     8761 ccccgggtga cgaagttacc ggaggcggct cccgtgggat ccaggtagat cgggaaggtg
     8821 taggggtact gcttgaggaa cccgtccacg gtcttggcat cctccttgac gctgatcgcg
     8881 gccaccgcga aggcctcgcc gcggagcgcg gagtggagct tttcgatcga gggcatctcc
     8941 tcccggcagg gggggcacca ggtggcccag aagttgagga gggtcacctt gcccttcagg
     9001 gccgcgggga ccagggcggg accgcccgcc cggggctgga cggagaagga gggcagttcg
     9061 acgggagcgg ggaagacgta gaacccgagg gcctcgaagc ggtccgcata ccagggcctg
     9121 gaggacgggg tctcgggcac cgaggcggaa acgggggcgg ccgcgggagc cccggacggc
     9181 acggctccgg ccggcgagtc ggaggacgaa gccgtcccgg tccgttcggc ccgggagcag
     9241 ccgcccgcga gaacggctac ggcgatcagg atcgggaaga cgtacttcaa ggttcgctcc
     9301 ttgggctcga gttgtatata gaaagtacta tgttgcaccg cccggatcaa gcccggcgag
     9361 atagccgccg accccccttt ttcgctatac taatttggag atccccgcca tccaagggcg
     9421 gccgaaacca gccaaggagt accgatgaaa tcccttcgct ccctacgaga catccttcgc
     9481 gccgagggcg aggaggagga taccgccccc gagaagatcg ccttccccga tcccctggcg
     9541 gagcgcttcc tcaagacccg gaccatcctg ctttccgggg aggtcaacaa ggagacggcg
     9601 gaaaaggtga tcagccggct ccttctcctg gaaagccagt cggacgaccc tatccgtgtg
     9661 ctgatcgatt cccccggggg ggacgtggac gcgggctacg cgatcttcga catggcccgc
     9721 ttcgtgaagc cccaggtctg gatgatcggc atgggactcg tggccagcgc cggcgccctc
     9781 atcctcctgg ccgcccccaa ggagcggcgg gtcgggcttc ccaactccca ctacctcatc
     9841 caccagcccc tttcaggtct ccggggcgtg gccacggaca tcgagatcca cgcccgggag
     9901 atcgagcgga cccgggaccg gatcaacaag ctcatcgccc aggagaccgg gcaggacctg
     9961 gagcgtgtcc agaaggacac ggaccgggac tactggatga ctgcggagga ggcggtggcc
    10021 tacggcctca tctcccgcac cgtcaaccga cgggacgaac tctagggcat ggaccgagca
    10081 cggatcgagg ccgccctggc gggcgcgtac cccggcctgc ccgacgcggc cgcgggcggc
    10141 cgccccgtgt accggggcaa ggtgcgggac gttgtggagg gtcccggccg ccttctcctt
    10201 gtggccagcg accggatctc cgccttcgac cgagtgctgt ccacgatccc cttcaagggc
    10261 gaggtcctca cccgggtctc ggcgtggtgg ttcgcccgga cctcggacat cgtccccaac
    10321 cacctggtcc ccccctcgca cctggggggc cgcgacccgg tcagggagac cggccgggcc
    10381 tgcctggtcc gcagggccga gatggtgccc gtggaggtgg tggtccgggg cttcctgacg
    10441 gggtcggcct ggcgggacta ccaggccggg cgcaccgtat ccggcatcgc cctcccctcc
    10501 ggccttgccc gggactcgga gttcccggaa cccctcctga ccccctccac gaaggaggcc
    10561 tccggccacg accgccccgc gagcggggag gagctcgtgc gatccggccg ggtggacgcg
    10621 gagctctggg aggagatctc ccgggccgcc cgggccctct acctccgggg acgcgaagtg
    10681 gcccgggagc acggcctcgt actggtggac accaagtacg agttcggggt cgccgacggc
    10741 cgcctggtcc tgggggacga gatccacacc cccgactcca gccgttactg gaggctcccc
    10801 ggcccgggcg cctccgcccc gggggcgcgg agcgagctgg acaaggaaag cttccgtcgc
    10861 tggctggccg aacgcggctt caccggggac ggcgaggccc cctcggtcac cgacgagatc
    10921 cgttcagaaa ccgcggcccg ctacgtggag gccttcgagg cgataacggg cgaggtcttc
    10981 gagccgaatc cccttccgac gcaagcgctg gacgaactgc tggcggagtg ggcctcgacc
    11041 gtctaaggac ggccgatccc cggatcgtga gcattccttt cgttcacgca tccggactcc
    11101 cggaggtccg acatgcgtat tccccgtctc ggcgcggccg cggcggccgc cctcgccgtg
    11161 ctttcgctct gctcctcccg ctgttccgtc ctggaccttc cccgcggaga gatctccgtc
    11221 ctttcctgga acctccagac cctcttcgat gaccgggacg acggggtcga gttcccgggc
    11281 tggtccgtgg ccaacgggca gtgggactcc gaggggtacc ggacccggct ccggaacttc
    11341 tcccgggccg cgtccgctgc ggtccccgga gggccggata tcctggtcct gcaggaggtc
    11401 gagaaccagc gggtggccga ggacctggcg gccctccttc ccggatcctg gcccaccctt
    11461 atcgtctccg ggggggaggg cgccgcgttc ggctgcgccg tcctgtcccg gtttcccgcg
    11521 gtcgtcgccc gggcacacgc ggtggacccg ggcccgggat tccgacccga ggatctgcgc
    11581 ccccttctgg aggtggtcct ggacgcggag gggatccgcc tgacgatcct ggcggcccac
    11641 tggaagagca aggtcggggg ggccgaggcc acggagcccg cccggagggc ccaggccgcc
    11701 ctggcggccc gacggatcgc cgagatcctg gcggaggatc cggccgcgga gctcctcctg
    11761 gcgggggacc tcaacgagaa ccccgacgaa tgggaacggg tcggccgcgc ctggcccacg
    11821 gccctcctgc ccgcggatcg tgcggcccgg gcggacggct ggaaagaccg gatcctcctg
    11881 gccgcggatc ccgggaacac cggtcctgga ccggacggac tcgtcctctg gagcccctgg
    11941 gaggaggcgg ggggcttcag ctacgtccac gaagggacgg aggaacggat cgaccatgtc
    12001 ctcctttgcc cggggctcct ggacgggaag ggcctggcct tctcgtcctt ctccgccgtg
    12061 accgagaggg aactgctttc ggaggacggg accccccggg cctgggatac ccggaaccgg
    12121 gaggggtatt cggaccacct ccccctgcgc ttggcgcttg cgtggcgata gcggggcgat
    12181 ccggaggtcc ccggcggcgc cgaatcccgt gtccccggca tccgaggggt gtccgcacgt
    12241 ccaggctccg gggtcgatgt caccgcggcc ggccggccgc gtatggccaa gcccgggccg
    12301 gccggggtac aattcccggg atatgtccga tcccaagccc ctgatcgtgc agagcgacct
    12361 gagcctgctg ctggacgccc acgatccggg cttcgagaag gcccgggagg acctgggtcc
    12421 cttcgcccag ctggagaaga gccccgagca tttccattcc taccgcatca cccccctgtc
    12481 cctctggaac gcggcggccg cgggtttcac cgccgaggac gccgccgcgg tcctgacccg
    12541 ctgggcccgg tacgaggtgc cccagagcgt gctctacacg atccgggaga tcgcctcccg
    12601 cttcggcaag ctcctgcttc gggaggggga tgcggcgggg gagctcctcc tgacctgcga
    12661 ggacaagcgg gtggccgcgg agatctcctc ctcccggcgc ctggccaagt ggctctcccc
    12721 ggcccccggg ggcttccggg tccgcctcac ggaccggggg acggtcaagc gggagctcac
    12781 ggccctgggc tggcccgtga aggacctggc ccccctggcg gagggggagc ccctggcctt
    12841 cgccctccgg gaaaccacgg gcgccggccg acccttctcc ctgcggccct accagaccga
    12901 cgcggtcagg gccttcctgg gggacaaggg gccgggtacg ggctacgggg tcgtggtcct
    12961 gccctgcggg gccggcaaga cggtggtggc catggccgcg gcggcccaga tcggccgcaa
    13021 gaccctggtt gtgacaacca acgtggccgc ggtccaccag tggatggacg aactggccga
    13081 caagacggac ctggcccccg gggacgtcgg ggagtatacg ggagcccgca aggaggtccg
    13141 tccggtcacc atagccacct accaggtcct ggtctggaga cccgacaagg aatccgacta
    13201 cccccacttc gacctcttcc ggcgcgagaa gtgggggctg gtgatctacg acgaggtcca
    13261 cctcctgccc gccccggtct tccgggtcgt ggcggagata caggcggtcc ggcgcctggg
    13321 gctcacggcc accctggtcc gcgaggacgg ccgggaggag gacgtcttca gcctgatcgg
    13381 ccccaagaag tacgacgtcc cctggaagga cctggagcgg aagggcttca tcgccgaggc
    13441 cttctgccgg gagatccggg tggccctgcc cgaggacgag gcggtccgct atgccgtggc
    13501 ggagacccgg ggcaagcacc ggatcgccgc ggagaatccc cggaagctcg agatcgtccg
    13561 ggagcttgtg gcgaaccacc ccgaggactc catcctggtg atcggccagt acctggacca
    13621 gctgggggcc atcgcccggg acctcggggc gcccctgatt accggggccg tgcccaacgc
    13681 ggagcgggag cggatctacg gggacttcaa ggccggccgg gtacgggtca tcgtggtatc
    13741 caaggtggcc aacttcgcca tcgatttgcc cgacgcctcc gtggccgtcc aggtgtccgg
    13801 ggccttcggg tcccggcagg aggaggccca gcggctcggc cggatcctcc ggcccaagga
    13861 gcgcaactcc tggttctact ccctggtgtc ccggttcacg ggggaggagg acttcgccgc
    13921 caaccggcac cgcttcctgg ccgagcaggg ctaccggtac accatcgaat cctgggacgt
    13981 cgacccgtgg aactgaagac cttgcccgcc ccggtctggc gggatgccct ggtgacggag
    14041 gacacggaag ccttcctgtc cgcggtgcgg aattacctcg gacccgtgcc cacgccctac
    14101 aacaagcatt ccctggtggc ccggctcgag gccttcctga agaagcccca gaccgcggaa
    14161 gcccaggcgg ccctcctgga ttccctggac gccctcatcc tgggggccct ggccgccgcc
    14221 ggcccctgcc cggagagctt cctccgggag ctcttcgcgg acgaacgcgg cccctgggag
    14281 atctccctgc gcctcgcgaa cctgcgggac cggctggtgc tcttccgggc cccgggcccg
    14341 gcggggcccg tccttgccgt gacgcccccc ctggcttccc gccttgcccg ggaggcggac
    14401 cttcggggtc tcctgggctg cgccccctcg gaaggacctc ccccgccgcc gcgggtggac
    14461 gcggacacga tctgcgccgc ggcctccatc ctcttccacg aggaaaaccc cctccgcagg
    14521 gacggccgac cggccaagcg ggtccgggcc cggctcctgg ccctcctacc ggagctgggc
    14581 ggcgcttcgg aaccggccgg cgcggacgag atcctggacg ccctgctcgc gggattccgg
    14641 ggtgccggtt tcctggaggg gccggagggg acgccggaca tggaccgcct ggccgccgcc
    14701 gcggaagcct ggggggatcg gctcccgttc ctcgtggccg cgggttcccg ggagctggac
    14761 ttccgccccg acgcggaggc cgggccgtcc ccgcgccctt ccctggcgga aagcgcccgg
    14821 gccctggagt ccatagccgc ggccctgccc acggggatcg cggtgagccg ggccggattc
    14881 cgccgacttg ccctgctctc ggcggagcct cgggggagcg cgggagcggg ccgcgctgag
    14941 acttcggcgg aagccctgct ccggttcggc ctggtcgaac ggacggcttc cgggatcgtc
    15001 cgggccgcgg cgtcccggcg ggtccgaagt acgatcccgg aagccggggg catcccctcc
    15061 ggacccgttc tggcggtgga ggcctcccac gccctgcggg tcctgcccga ggcggatccg
    15121 gcggcccggg tcttcgcggg cacggtggcc cgactggaga gccgggggga gttgtggtcc
    15181 tgcgtcctgg accggaagag cgtccgccgg gcactggacg cgggcctggg atccgagcgc
    15241 atcgcccgga ccctggagag gctctccggc aggccccttc cccagagcct ggcttactcc
    15301 ctgacgggct gggaaaaaga gcacctttcg gttaggctat actacgggta tgtcctggcc
    15361 gcggagggac cggcccggac ccgggcggag aacgccccgg ccctggcggg gctccgggga
    15421 ccggccgtgg cggagggagc ctggctcctg gagtccgagg atcccgggga gatccggacg
    15481 gccctggaaa aggcgggtct ccacgttccc cggacccgaa ggtccggccg gccggaacct
    15541 tccgggacga acgcttccgg aagcggagcg gccggcctgc ttgcaccggt tacgcaggcg
    15601 gagaggcccg cctggtcgcg gctcctggac ggccccgtag atcgggccgg ggaaccggga
    15661 ccggcgcggt ccccggattc cctggcccgg cttcgggccc ggctggggga actggacctt
    15721 ccggagggac gcagggccga gctcgccgag cggatcggcc gcaggatcat cctgaccgag
    15781 gaccagctga tccgggcgga gacggacggc ccggggctgg aggccggtgc cctggattac
    15841 ctgggcaagg tgcgggtcgt ggaacgggtc ctccgttcgg gcggcgccct cctggagatc
    15901 ctgacccgga gcccggacgg ccggccggag ctgttccggg tccgtcccct ggagctggag
    15961 aagaccgacc gggggctggt cctccgggca gccgacgccg aggacgggag cgtccggatc
    16021 ctgcccgtgc gttccatgag ccacgtgaaa cggatcaaga ctaccttatt tggagattga
    16081 gatgactacg atgcccaaca agaacaacga tacgatgcag ctgaagctgg cggtcctcat
    16141 cgacgcggac aatacccagg cctggatcgt ggacggcctc ctggcggagg tggccaagta
    16201 cgggatcgcc agcgtgaagc gcatctacgg cgattggacc accacgaacc tccgacactg
    16261 gaaggacaag ctcctggcct acgccatcca gcccatccag cagttcgcgt acaccagcgg
    16321 gaagaacgcc acggactcgg ccatgatcat cgacgccatg gatcttttgt actccgagcg
    16381 cctggacggg ttctgcgtgg tgacctccga ctcggacttc acccgcctgg cggcccggct
    16441 ccgggagtcc ggcaagctgg tcctgggctt cggggagaag aagaccccca agcccttcgt
    16501 ggcggcctgc gac
//
LOCUS       DAOTEI010000069        16321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2412_length_16321_cov_4.759560, whole genome shotgun sequence.
ACCESSION   DAOTEI010000069 DAOTEI010000000
VERSION     DAOTEI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 16321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16321
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2412_length_16321_cov_4.759560"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1949
                     /locus_tag="P5313_08280"
     CDS             147..1949
                     /locus_tag="P5313_08280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY80399.1"
                     /translation="MSRFLFVPSVARGNGSGHLVRCLTLARELSDLPGSSAAVFLDED
                     PGPGSRGAREIEAAFPERVRGLSLVRSRAAAETGTWAFVVLDLRNASREEELAWRALA
                     PTVALDEGGPAALGASFRIDALPRTGREALLGDANLESRGFLHLPGRRRDSPPDRFGN
                     ILLAFGGEDPRGLGPATARALVAGGFFRPESLTLLTGPLGDASRRVPRGVRIAGPVPD
                     LKETLASWDLVFTQFGLTAFEAAWAGCAVILVDPGRYHARLSRSAGFPAAGIGRPEPR
                     ALARLLSDPAALAASTAAAAPKCAESLAGRLAALRPGASGSCPVCGARERAVLERYPR
                     KSYFRCAGCGMVYRELFEGGDTPYVESYFFEEYRRQYGRTYLEDFPALTAFAAGRLEI
                     LERLLPREAGGRRPVILDVGCAYGAFLAEAARRGWDARGLDIAPSAAAYVRDTLGLPA
                     RAGDFLDPGTRSDLGGPVDCLSMWFVIEHFEDLARALRAAADLVRPGGIFAFSTPSGG
                     GISARSDPGRFFDRSPDDHATVWEPRRAAGILGPFGFRVERIRVTGHHPERFPGALGK
                     PGAARMAGAASRLFGLGDTFECYARRVRDSERED"
     gene            1950..3539
                     /locus_tag="P5313_08285"
     CDS             1950..3539
                     /locus_tag="P5313_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRY80400.1"
                     /translation="MRTIFILGAGVMQIPALRIAKERGWRVVAADGSDKAVGIPFADE
                     FLHIDLKDRPALEEAARRLRENGGLDGVMTAGTDFSASVAWVAEKLGLPGIPFETALN
                     ASDKSRMRAVFSAAGVPSPRFSVLSRSDSPAALPDFLAFPLVVKPVDNMGARGCRLVR
                     DGTELAEALADAHGHSRSGRAIVEEYLEGPEFSLDALVHEGRVRLCGIADRHITFAPY
                     FVEMGHTMPTAFPERDVAEVVRVFEAGIRALGIRNGAAKGDIKLTARGAYVGEIAARL
                     SGGYMSGWTYPYASGVDPAAGALDIAVGREPRDTEPKRNWVSAERAFISIPGIVASVS
                     GERAARAEPYVKEVFFRVAAGDRVVFPANNVEKCGNVISQAPDRESAVAAAEGAARAV
                     RIRLAPRNPDTDAFLSGGGIPNPDGTTWPASAFRVSPALAAAAAFLPDRGIPPRSGSA
                     PSPRPRVAYAALPGAEADTARDWQGRSIAESLKVVQDWTGAALDDGRAELVLGASFWK
                     ALFRGGAQGAAYVIDSLFRKD"
     gene            3550..4665
                     /locus_tag="P5313_08290"
     CDS             3550..4665
                     /locus_tag="P5313_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY80401.1"
                     /translation="MRSLAAAVLLTLLATARVAGQGTAGTGAPPAPGPTAPAAPAAPS
                     PSASPRPLASAAADLGARLTWDPLTATGYLERAGHRAAFKLGAPQWVLDGTELARIDP
                     VTLRDGVPVLSGAAAAALGTWFTRKEEERASQFHVAAILIDPGHGGKDPGALADHTLG
                     GVKKRIVEKDIALTVALRVRDRLKERWPARTILMTRDSDVFPTLEERVEIANSLELGR
                     NDAIIYVSIHANASFNKNARGFEVWYLNPDYRRKLVDGSTLPATDKDIAPILNAMLEE
                     EFTTESVLLAKRILDGMGTVIRDASPNRGLRAEEWFVVRNAKMPSVLVEVGFLTNPQD
                     AALLADAAYLRSLGDGIYTGIVDFVDYFERRKGPASP"
     gene            4662..5186
                     /locus_tag="P5313_08295"
     CDS             4662..5186
                     /locus_tag="P5313_08295"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRY80402.1"
                     /translation="MIPDLRNRLTAILERLLAPRILWPGLLFLSLLLSVAGWFLRPLP
                     TRSAVLFFPRAEDLRLSAETRQILPGGPGLEEGAREVVEEFLLGPGDSRMIAALPRGT
                     RLREVLYRRGRLYVDLSEDAIFGLEPSLDLGLRAIRKTLRYNFPTLGTVIVTVGGREP
                     GVQYLGADREPKKK"
     gene            5270..6349
                     /locus_tag="P5313_08300"
     CDS             5270..6349
                     /locus_tag="P5313_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HRY80403.1"
                     /translation="MRRQFLIVAAALLLVGAVGVFADEAVLIDFSLLKADIIEDPVQQ
                     GKLTQNRATMIDFSSTAGASYTEDQKKMMRTSLAIPNWSVVLASSSRSAVTVPNSYAR
                     EAPVNQEAKQFAGQTVMGVRVSFPLEPFNSWARVTPPFEIPSFEPKADVADDGTITPK
                     AAGSGGDPVNNRLTRFEGTYNADTKVQTALGVVKNVGVIKSVAVMVKGLNFPHGLSLI
                     LKDQDANEKVIFMGYLNFDGWRELRWDNPQYIRDVRNRELRIYPLYPKTSPFVKLGGI
                     LFSRDAAHEGGDFVAYIKDVKILYDKAILDPVTDIDDEAVWGIVGVKEAERKRIESQR
                     FGNSQVLRYIESLKQEQKSEFTPSK"
     gene            6432..7676
                     /locus_tag="P5313_08305"
     CDS             6432..7676
                     /locus_tag="P5313_08305"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY80404.1"
                     /translation="MNEFETLNRAELEERYERLLPVYQAVLADMSVRVSKCLQEAGIH
                     PTLKSRVKSFPSWFRKRILLIQEARAAGRKSVKPIADVLALRVICPFMGDLQKVEAEI
                     GRCFRVVEVERKGADRSFREFGYESTHILVEIPVDLRRASKGLDLGVCEIQVRTILQD
                     AWAEVEHELVYKSEFNPFDEPMKRKLAALNANLSLSDIIFQELRDYQHRLVNELDRRR
                     TNFFRKIEKSIDEPFFPERADDLTLGAQAPTAAEYSGCESSIDEMLVFALNAHNRDDY
                     AAAIETYSCILSREPKDEIAALIHKHRGMAYFAESRYEEAIADFSRTLDLDPRCYKAV
                     YYRGVVHSVLADYSAAVRDFELALEIHPFHFYSLYRRSQAYFHLGDYPKALSDCEAAL
                     RLDPENEQAAKMKALILDRLRM"
     gene            7761..7832
                     /locus_tag="P5313_08310"
     tRNA            7761..7832
                     /locus_tag="P5313_08310"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7794..7796,aa:Glu,seq:ttc)
     gene            8197..8658
                     /locus_tag="P5313_08315"
     CDS             8197..8658
                     /locus_tag="P5313_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80405.1"
                     /translation="MKKVLGIAAALVLLAGIPAFAAGPAGLTGFGVYGSVGSASGSLS
                     GGVGLSFKWASFPVVGLKYDFTGERFGASVDYYVIDAEGLGSGLSYFLGAGLYAGIAG
                     GEFDFGLRIPFGLQFWPVKKFELYLSPVLSIPLIPAPSVGIGAEFGARIRF"
     gene            8786..9145
                     /gene="trxA"
                     /locus_tag="P5313_08320"
     CDS             8786..9145
                     /gene="trxA"
                     /locus_tag="P5313_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRY80406.1"
                     /translation="MPEKLTTEDFKKKVFDYESKKEWEYAGDLPAIIDFYADWCGPCK
                     MIAPVLEKVAKKYEGKIHVYKVDTEAEQELAGVFGIQSIPTLYFVPKEGNPSYAMGAL
                     PQGQIERAVSEVLKVEA"
     gene            complement(9165..10331)
                     /gene="thiI"
                     /locus_tag="P5313_08325"
     CDS             complement(9165..10331)
                     /gene="thiI"
                     /locus_tag="P5313_08325"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HRY80407.1"
                     /translation="MDTLYLIKPGELELKTGNLREFESRLRKDIERRLEGRARVDIRP
                     GRFFLRVPEGLEERAERVLSRVPGIVGYARALTCGKTMPEIGELALRVAREGREAGHS
                     TFKVEARRADKGFPLDSYGLARELGGLILENVPGLSVDVKKPEFTVFVEVRERAYVHG
                     AIRKGQKGLPSGSAGKGLLLLSGGIDSPVAGYLMAKRGLSLEAVYFNAYPYTSREAWE
                     KVRDLAGIVARYAGNLVLHTLSFTDIQMEIKRGAPVECSTLFLRAAMVRAAHLLAAEI
                     GANSLVTGESLGQVASQTAENIRFSQARTDLPIFRPLIGVDKEDTMRMAREIGSYEIS
                     ILPYEDCCVLFSPEHPILRSRPDREGPIYESLGLDRLIEEAVRTRETLPVPFSV"
     gene            complement(10340..11491)
                     /locus_tag="P5313_08330"
     CDS             complement(10340..11491)
                     /locus_tag="P5313_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRY80408.1"
                     /translation="MSRTYLDWAATAPPSPEALEEYLRAAREYPGNPSSLHAEGSAAR
                     TALEDARARLAGLLGCRPGRVVFTSGGTEADGLPLLALLRRPSPGSVVISSIEHPAVF
                     EQAAVLERLGWKVRRVDPGPDGRVAPGAVVDACGPDTVLAAVMAVNNETGAVQPLSEI
                     RLALDEASRGGRRIRFHTDAVQAFGKVPFEPESLGADSAALSAHKIRGPRGVGALYLR
                     TPIEPLAAGGGQEGGIRSGTENLAGILAFERAAAAAIRDLGENRRRARVLSDRLLDHI
                     RAIPDVRPVPECRKAGDERYSPWILGLSLPGLAGETAVRILSDAGYDVSTGSACSSSR
                     RKRRVLEAMGVPEALAFCAIRVSLGPTTTESEIDGFAEALRDAYLRYRT"
     gene            complement(11517..11849)
                     /locus_tag="P5313_08335"
     CDS             complement(11517..11849)
                     /locus_tag="P5313_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HRY80409.1"
                     /translation="MPAPKTLEDTRRFADMVAAKQGWTVNPDAEFTGTLVEGLTTNWN
                     RYGYYLCPCRDTEGSREADASVLCPCRYARADVAEHGHCFCALFLRPDFAAAGREVQG
                     IPDRRSSG"
     gene            complement(11850..12149)
                     /locus_tag="P5313_08340"
     CDS             complement(11850..12149)
                     /locus_tag="P5313_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRY80410.1"
                     /translation="MLDTLEYTKVEGENKQHRVTVYALSTCGFCKRAMAFLDTHKFEY
                     SYIYMDKVPLETKTEAKKILKDRYKQDVAFPFAVVDDRESLVGFIEADWKRTLEV"
     gene            complement(12239..13594)
                     /locus_tag="P5313_08345"
     CDS             complement(12239..13594)
                     /locus_tag="P5313_08345"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRY80411.1"
                     /translation="MIVEPERLSVLKPGPPGRGDFVLYWMQHAQRARENPALEYAASE
                     AARLRLPLAVLFVLAPYPSATAVHYRFLLGGLAETARALEARGARFILRRGESPKVAA
                     ELSRRAALAVCDAGWTRPELAWRREFAERSACPVIRVDGDAVVPVEAASDKLEWSAFT
                     LRRKIEGAVPAYAARIPPVVELRRDARGLDLDGGAELLRTEGGPLRPEYPGDRVPEQT
                     ALPPPGEEAARTRFDTFLNTGLDRYDADRNDPTLDGTSGMSPYLHFGQVSPVLLARKV
                     LERGGAGAASFIEQLTVRRELSLNLVRFLPDAYDRWEGLPDWARRTLRDAAGDKRDYL
                     YSRSDFEAARTHDPYWNAAQNQLVRSGSIHNYMRMYWGKKILEWSADPREAYGTALYL
                     NDRYALDGRDPNGYAGVAWCFGRHDRPWAGRAVFGTVRYMNAAGLRRKFDADVYAKAW
                     A"
     gene            13753..15564
                     /gene="lepA"
                     /locus_tag="P5313_08350"
     CDS             13753..15564
                     /gene="lepA"
                     /locus_tag="P5313_08350"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRY80412.1"
                     /translation="MRDPNHIRNFCIIAHIDHGKSTLADRFIQMAHIVDDRKFQNQML
                     DNMDIERERGITIKSQAVSIPYTASDGITYTLNLVDTPGHVDFSYEVSRAISSCEGAI
                     LLIDASQGVEAQTLSNMYMALEHDLEILPVINKIDMPAADVPGVRDQISHDLGLDGDE
                     ALPVSAKLGTGLPDLFEAIVRRFPPPRGSADKPARALIFDSRYDPFRGVLVHIRVVDG
                     RIQPGDRILLMNTGAVHEVEEVGFFRIELSKTEELSAGDVGYVVAGIKTVSDVRVGDT
                     ITNADVPASEPLPGFREVKPVVFSSIYPMDTNDYDELKIAMEKLKLNDASLVYDKDNS
                     VALGFGFRCGFLGLLHLEVVQERLEREFDQSIIMTAPSVRYKILLKNGQELFVDNPSE
                     YPDPGRIETAEEPFIKAQIITPVAYVGNLISLCVQKRGVQTSFTHLDEKRVEMIFEMP
                     LAEVLFDFYDKLKSTSRGYASFDYDVIGYHPSELVKLDILINGKPVDALSQLVFKPGA
                     YERARKVCERLQGAISRQQFKIAIQGAIGGTIIARETVNPVRKDVLAKCYGGDITRKR
                     KLLEKQKEGKKRMKSVGDVDLPQEAFLAVLRTNEDED"
     gene            complement(15565..>16321)
                     /locus_tag="P5313_08355"
     CDS             complement(15565..>16321)
                     /locus_tag="P5313_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY80413.1"
                     /translation="LEKPFASTVREAETLIRASEAAGLPLAVFHNRRWDADFRSLSGL
                     LDRDVLGEVSEAEFRWERFRPSVQDRWKERPGPGSGLLWDLGPHLFDQACRLFGPFDW
                     VMADVAAQRPGTRVDDWFHAVLGSGKRRIILHAGCLAPDTGFHYRVHGAKATFRTFGE
                     DGQEAVLRAGDLPGSPGWGTMPGAGLLSRPDGSEERMDLAGGDWREFYRGLAAALAGR
                     GPLPVETAGALETLRLMEAAKVSSATGRRVTLG"
BASE COUNT         2589 a         5604 c         5480 g         2648 t
ORIGIN      
        1 ctccgcgccc ccgaagcccg ggtgaccgtg gatacccggg aggattacga ggctgcgcgg
       61 gccctcgccg aggaatgcgg cttcgaccct acctcggccg ccttggttcg ccggctcctc
      121 ggcctcggcg ggaaggccta gccgccgtgt cccgtttcct cttcgtgccc tccgtcgccc
      181 ggggcaacgg ctcgggccac ctggtccgct gcctgaccct ggcccgggag ctctcggacc
      241 tgccgggatc ctccgccgcg gtcttcctgg acgaggaccc gggacccggg tcccggggag
      301 cccgggagat cgaggccgcc ttccccgaaa gggtgcgggg cctgagcctg gtccggtccc
      361 gagcggcggc ggaaacgggg acctgggcct tcgtggtcct ggatctgcgg aacgcttccc
      421 gggaggagga gctggcctgg cgcgccctgg ctcccacggt cgccctggac gagggcgggc
      481 cggccgccct cggggcttcc ttccggatcg acgcgctgcc ccggacgggc cgggaggccc
      541 tcctcggcga tgccaacctg gaatcccggg gcttccttca tctcccgggc cggcgccggg
      601 actccccccc ggaccgcttc gggaacatcc tcctggcctt cgggggggag gatccccggg
      661 ggctcggtcc cgccacggcc cgagccctgg tggccggcgg gttcttccgt cccgagtcgc
      721 tgaccctcct gaccggcccc ctgggggacg cgtcccgccg ggtgccccgg ggggtgcgta
      781 tcgcgggacc cgtcccggac ctcaaggaga ccctggcttc ctgggacctc gtgttcacac
      841 agttcggcct gaccgccttc gaggcggcct gggcgggctg cgcggtcatc ctggtcgacc
      901 ccggccggta ccacgcccgg ctttcccgga gcgcgggttt cccggccgcg gggatcggcc
      961 gtccggagcc ccgggccctc gcccgtctcc tttcggaccc ggcggccctg gccgcgtcga
     1021 ccgcggcggc cgccccgaaa tgcgccgaat ccttggccgg tcgcctggcg gccctccggc
     1081 ccggggcctc cgggtcctgc ccggtttgcg gcgcccggga acgcgcggtc ctggagcggt
     1141 atccccggaa gagctatttc cgctgtgccg ggtgcgggat ggtctaccgg gagctcttcg
     1201 agggcgggga cactccctac gtggagtcct attttttcga ggagtaccgg agacagtacg
     1261 gccggaccta tctggaggac ttcccggccc tgacggcctt cgcggcgggg cggttggaga
     1321 tcctggagcg gctccttccg cgcgaggccg ggggccgccg ccccgtgatc ctggacgtgg
     1381 gctgcgcgta cggggccttc ctggcggagg ccgcccggag gggctgggac gcccggggcc
     1441 tggacatcgc cccgtccgcg gcggcctacg tgagggacac cctgggcctc cccgcccggg
     1501 cgggggattt cctggatccc ggtacgcgct ccgacctggg cggccccgtg gactgccttt
     1561 cgatgtggtt cgtgatcgag cacttcgagg acctcgcccg ggccctccgg gcggcggcgg
     1621 acctggttcg ccccggcggg atcttcgcct tctcgacccc ctcgggcggg gggatctcgg
     1681 cccgctccga tcccgggcgc ttcttcgatc gcagccccga cgaccacgcc acggtctggg
     1741 agccccgccg ggccgcgggg attctcggac ccttcggttt ccgggtagaa cgtatccggg
     1801 tcaccggcca ccatccggag cgcttcccgg gagccctggg aaagcccgga gccgcccgga
     1861 tggccggggc cgcgtcgcgg ctattcggat tgggggacac cttcgaatgc tacgcccggc
     1921 gggtccggga ttccgaacgg gaggattaag tgcggacgat cttcatttta ggcgccggag
     1981 tcatgcagat ccccgccctc aggatcgcga aggagcgggg ctggcgggtc gtggccgcgg
     2041 acgggagcga caaggccgtg ggcatcccct tcgccgacga gttcctgcac atcgatctca
     2101 aggaccggcc cgccctggag gaggccgccc ggcggctccg ggagaacggt ggcctggacg
     2161 gggtgatgac cgcgggcacg gacttttccg cctccgtggc ctgggtggcg gaaaagctgg
     2221 gtctgcccgg gattcccttc gagaccgccc tgaacgccag cgacaagtcc cggatgcgcg
     2281 cggtcttctc cgccgcgggg gtgcccagtc cccgcttttc cgtcctctcg cgctcggatt
     2341 cccccgccgc cctgccggat ttcctggcct tccccctggt ggtcaagccc gtggacaaca
     2401 tgggcgcccg gggctgccgg ctggtccggg acgggaccga gctggccgag gccctggcgg
     2461 acgcccacgg gcattcccgg tcgggaaggg ccatcgtgga ggaatacctt gaggggccgg
     2521 agttcagcct ggacgccctg gtccacgagg gaagggtccg cctctgcggg atcgcggacc
     2581 ggcacatcac cttcgccccc tacttcgtgg agatggggca taccatgccc acggccttcc
     2641 cggagcggga cgtggccgag gtggtccggg tcttcgaggc ggggatccgg gccctgggaa
     2701 tccggaacgg ggcggccaag ggggacatca agctgaccgc ccggggggcc tatgtgggcg
     2761 agatcgcggc ccggctttcg gggggctaca tgtccggctg gacctatccc tacgcctccg
     2821 gggtggatcc cgcggcaggg gccctggaca tcgccgtggg ccgggaaccc cgggacacgg
     2881 agccgaagcg gaactgggtc tccgcggaac gcgccttcat cagcatcccg gggatcgtcg
     2941 cctcggtctc cggggaacgg gccgcccggg ccgaacccta cgtcaaggag gtcttcttcc
     3001 gggtggccgc cggggaccgg gtagtcttcc cggccaacaa cgtggagaag tgcggcaacg
     3061 tgatctccca ggccccggac cgggagtccg cggtcgccgc ggcggaaggg gccgcccggg
     3121 cggtacggat ccgcctggct ccccggaacc cggacaccga cgccttcctc tcgggcgggg
     3181 gcatccccaa tcccgacggc acaacctggc cggcctcggc gttccgggta tcgcctgccc
     3241 tggcggcggc cgcggccttt ctcccggacc ggggaatccc tccccggtcc gggtcggcgc
     3301 cctccccccg gccccgggtc gcctacgcgg ccctgcccgg cgcggaggcg gatacggccc
     3361 gggactggca gggccgctcc atcgccgagt ccctgaaggt cgttcaagat tggaccggcg
     3421 ccgccctaga cgacggccgg gcggagctcg tgctgggcgc ctcgttctgg aaggccctgt
     3481 tccggggtgg agcccaggga gccgcctacg tgatcgattc cctgttccgg aaggactgag
     3541 ggaggcgggg tgaggagtct ggcggccgcg gtcctgctga ccctgctggc gacggcgcgc
     3601 gttgcgggtc agggcacggc cgggacggga gcccctcccg ccccggggcc taccgccccg
     3661 gccgcgcccg ccgctccgtc gccgtccgcc tcgccgcggc ccctggcctc cgcggccgcg
     3721 gacctgggcg cccggctcac ctgggaccct ctcacggcaa cggggtacct ggagagggca
     3781 gggcaccggg ccgccttcaa gctcggggcg ccccagtggg tcctggacgg aacggagctg
     3841 gcccggatcg atcccgtgac gctacgggac ggggttcccg tgctttcggg cgcggcggcc
     3901 gcggcgcttg gaacctggtt cacccggaag gaggaggaga gggcctccca attccatgtg
     3961 gccgcgatcc tgatcgatcc gggccacggg ggcaaggacc ccggtgccct ggccgaccat
     4021 accctgggag gggtcaagaa gcggatcgtg gaaaaggaca tcgccctgac cgtagccctt
     4081 cgggtccggg accgcctcaa ggaacgctgg cccgcccgga ccatcctcat gacccgggat
     4141 tccgacgtct ttccgacctt ggaagaacgg gtcgagatcg ctaattccct ggagctcgga
     4201 cggaacgacg cgatcatcta cgtgtcgatc cacgccaacg cctcgttcaa caagaacgcc
     4261 cggggcttcg aagtctggta cctgaacccg gattaccgtc ggaaactcgt ggacggatcc
     4321 accctcccgg ccacggacaa ggacatagcc cccatcctga acgccatgct ggaggaggag
     4381 ttcaccaccg agagcgtcct tctggccaag cgcatcctgg acgggatggg tacggtcatc
     4441 cgggacgcgt cccccaaccg gggcctccgg gcggaggagt ggttcgtggt ccggaacgcc
     4501 aagatgccca gcgtcctggt ggaggtgggg ttcctgacga atccccagga cgccgccctc
     4561 ctggcggacg ccgcttactt gcgctccctc ggggacggca tatatactgg gatcgtcgac
     4621 ttcgtcgact acttcgaacg acgcaaagga cccgcttccc catgattcca gacctccgga
     4681 acaggcttac ggccatcctg gaacgcctcc tcgctccccg gatcctgtgg cccgggctgc
     4741 ttttcctctc gcttctgctc tcggtcgccg gctggttcct gcgtcccctg cccacccggt
     4801 ccgcggtcct ttttttcccc cgggccgagg atctccggct ctcggcagag actcggcaga
     4861 tcctgcccgg agggccgggt ctggaggaag gggcccggga agtcgtggag gagttcctgc
     4921 tgggccccgg ggattcccgg atgatcgcgg cccttccccg gggtacccgc ctccgggagg
     4981 tcctgtacag gcgaggcagg ctctacgtgg acctttccga ggacgcgatc ttcggccttg
     5041 aaccgtccct ggacctgggg ctccgggcga tccgcaagac cttgaggtac aacttcccga
     5101 ccctggggac cgtaatcgtc accgtggggg gccgcgagcc gggagtccag tacctcggcg
     5161 cggaccggga gcctaaaaaa aagtaaaaat aattgacaaa gcacttgcgg cgactataat
     5221 tccaggtaat tccttgattc tgagcatgct ttatgaaagg agcacgttaa tgagaaggca
     5281 gttcctcatc gtggccgcgg ctcttctctt ggttggtgcc gtcggcgtct tcgcggacga
     5341 agctgtcctc atcgatttct ccctcctgaa ggccgatatc atcgaggatc cggtgcagca
     5401 gggtaagctg acccagaacc gcgctacgat gatagatttc tcttccaccg cgggggcgag
     5461 ttacactgag gatcagaaga agatgatgcg cacctccctg gccatcccca attggtccgt
     5521 ggtgctggca tcctcctccc gctccgccgt gacggttccc aattcctatg cccgggaagc
     5581 cccggtcaac caggaagcca agcagttcgc cggccagacc gtcatgggcg tccgggtgag
     5641 cttccccctg gagcccttca actcctgggc ccgggtcacc ccgcccttcg agatcccctc
     5701 cttcgagccg aaggcggacg tcgcggacga cgggaccatc accccgaagg ccgcgggctc
     5761 cggcggtgat ccggtcaaca accggctgac ccgcttcgag ggcacctaca acgcggacac
     5821 caaggtccag accgccctgg gcgtcgtcaa gaacgtgggg gtcatcaagt ccgtggccgt
     5881 catggtcaag ggcctcaact tcccgcacgg cctgtccctg atcctcaagg accaggacgc
     5941 caacgagaag gtcatcttca tggggtacct caacttcgac ggctggcggg agctgcgctg
     6001 ggacaatccc cagtacatcc gggacgtccg caaccgggaa ctccggatct acccgctcta
     6061 cccgaagacc agccccttcg tgaagctcgg ggggatcctg ttctcccgcg acgcggccca
     6121 tgagggcggg gatttcgtgg cctacatcaa ggacgtgaag atcctctacg acaaggccat
     6181 cctggacccc gtcacggaca tcgacgacga ggccgtgtgg ggcatcgtgg gcgtgaagga
     6241 agccgagcgc aagaggatcg agagccagcg cttcgggaac tcccaggtcc tgcgctacat
     6301 cgagagcctc aagcaggagc agaagtccga gttcactccc tccaagtaag gagtgccgga
     6361 atcggaaggg gccgccctcg ggcggccctt ttcttttgcc ttttcgggcc gctccgggta
     6421 tcatagcttc gatgaacgag ttcgaaaccc tgaaccgcgc ggagctggag gaacgatacg
     6481 agcggctcct ccccgtttac caggccgtgt tggccgatat gtccgtccgg gtatccaagt
     6541 gtctccagga agcgggcatc caccccaccc tgaagtcccg ggtgaagtcc ttccccagct
     6601 ggttccgcaa gcggatcctg cttatccagg aggcgcgggc cgccggacgg aaatccgtca
     6661 agcccatagc cgacgtcctg gcgctccggg tcatctgtcc cttcatgggg gacctccaga
     6721 aggtcgaggc cgagatcggg cgctgcttcc gggtagtgga ggtggaacgg aagggggcgg
     6781 accggtcctt ccgggaattc ggctacgagt ccacccatat cctggtggag atccccgtgg
     6841 acctccggag ggcgtccaag ggcctggacc tcggtgtctg cgagatccag gtgcgcacca
     6901 tcctgcagga cgcctgggcc gaggtggagc acgaactggt ctacaaatcg gagttcaacc
     6961 ccttcgacga gcccatgaag cggaagctcg ccgccctgaa cgccaacctc tccctctcgg
     7021 acatcatctt ccaggaacta agggactacc agcaccgcct ggtgaacgag ctggaccggc
     7081 ggcgtaccaa ctttttccgc aagatcgaaa agtccatcga cgagcccttc ttccccgagc
     7141 gtgcggacga ccttaccctg ggcgcccagg cccccaccgc ggcggaatac tcgggctgcg
     7201 aatccagcat cgacgagatg ctggtcttcg cgcttaacgc ccacaaccgg gacgattacg
     7261 ccgcggcgat cgagacgtat tcctgcatac tgtcgcggga accgaaggac gagatcgccg
     7321 ccctcatcca caagcaccgg gggatggcgt acttcgccga gtcgaggtac gaggaggcga
     7381 tcgctgactt ttcccggacc ctggacctgg atccccgctg ctacaaggcg gtctactacc
     7441 ggggagtcgt ccattcggtc ctggccgact attccgccgc ggtccgggat ttcgagctgg
     7501 ccctggagat ccaccccttc catttctact ccctgtaccg gaggagccag gcctacttcc
     7561 atctcgggga ctacccgaaa gccctgtcgg actgcgaggc cgccctgcgt ctggatccgg
     7621 agaacgagca ggccgccaag atgaaggccc tgatcctgga ccgcctccgg atgtgatcct
     7681 tcgggatcct cccttccctt gacagggaag gccgtttttc tgtaaggtgt ggctccggcg
     7741 cgcagcgccg gagagcgttt gtccccttcg tctagtggtt aggacagcgg gttttcatcc
     7801 cgccaacagg ggttcgactc ccctagggga tggagagata agccggccga aaggccggtt
     7861 ttttcatgcg tgcgacttag gggagtcgaa cccgacgcgc cgttccggaa tcgcgcgatc
     7921 ctttgagcgc gatgccggag gagccgcaag gatgccgtcg tgcgcgatag cgcacacgac
     7981 aatcgtttcc gctcggcaag cggagcggag cgacgcgtgc ggcgacaggc gcggaggcgg
     8041 gttccggggc cgcgacacga tgtcgcggcc ccggaactcg actcccctag gggatggaga
     8101 gataagccgg ccgaaaggcc ggttttgttt tccgcttctt tgcatccatc cggattcgcg
     8161 gtatattgtc tcgaaagcac cctaacggag gtcgtgatga aaaaggttct tggtatcgca
     8221 gccgccctgg tcctgctggc gggaattccc gccttcgccg cgggtcccgc gggactgacg
     8281 ggattcggag tgtacggaag cgtgggaagc gccagcggtt ccctgagcgg cggcgtgggc
     8341 ctctccttca agtgggccag tttcccggtt gtggggctca agtacgactt caccggggag
     8401 cgtttcgggg cctccgtgga ttactacgtg atcgacgcgg agggtctggg ctccggcctg
     8461 agctacttcc tcggcgcggg cctctacgcg ggcatcgcgg gaggggaatt tgatttcggc
     8521 ctccgcattc ccttcggtct ccagttctgg ccggtcaaga agttcgagct ctatctttcc
     8581 ccggtgcttt cgatccccct cattcccgca ccgtccgtgg gtatcggcgc ggagttcggc
     8641 gcccggattc gcttctagat cccgttcgtt catcgaaggg accgcctcgg cggtcccttt
     8701 ttttcgggcg gcccttgtcg aaacatatga aaaacactat attaagtatg tccacaagtt
     8761 caaacgaagg cgcaaggaga cacggatgcc cgaaaaactg actacggaag acttcaaaaa
     8821 gaaggtattc gattacgagt ccaagaaaga atgggaatac gccggggacc tccccgcgat
     8881 catcgacttc tacgcggatt ggtgcgggcc ctgcaagatg atcgccccgg tactggaaaa
     8941 ggtcgccaag aagtacgagg gcaagatcca cgtctacaag gtggacaccg aggcggagca
     9001 ggagctggcc ggagtcttcg ggatccagag catccccacc ctctacttcg tccccaagga
     9061 ggggaatccc agctacgcca tgggagcact gccccagggg cagatcgagc gcgccgtctc
     9121 cgaggtcctc aaggtggagg cctgatcctc aggccgacag gcttctagac ggaaaacggg
     9181 acgggcaggg tttcccgggt ccggacggcc tcttcgatca ggcggtccaa acccagggac
     9241 tcgtagatcg gcccttcccg gtccggccgg gacctcagga tggggtgttc cggggagaag
     9301 agcacacagc aatcctcgta gggcaggatc gagatctcgt agcttccgat ctcccgggcc
     9361 atccgcatgg tatcctcctt gtccaccccg atgaggggcc ggaagatcgg aaggtccgtc
     9421 cgggcctggg agaatcggat gttctccgcg gtctggctgg ccacctgtcc taggctctcc
     9481 ccggtcacca gggagttggc tccgatctcc gcggccagca ggtgggcggc cctgaccatg
     9541 gccgcccgga ggaagagggt ggagcactcc acgggagccc ctcgcttgat ctccatctgg
     9601 atatccgtga acgagagggt gtgcaggacc aggttgcccg cgtacctggc cacgatgccg
     9661 gccaggtctc ggaccttctc ccaggcttcc cgggatgtgt agggataggc gttgaaatag
     9721 accgcctcca gggaaagtcc ccgcttggcc atgagatagc ccgccacggg ggagtcgatg
     9781 ccccccgata acagaagcag tcccttcccc gcggagcccg agggaagccc cttctggccc
     9841 ttccggatgg cgccgtgcac gtaggcccgc tcccggacct ccacgaaaac cgtgaactcc
     9901 ggtttcttca catccaccga aagcccgggt acgttctcca ggatgagccc tcccagttcc
     9961 cgggccaggc cgtaggaatc cagggggaag cccttgtccg ccctgcgggc ttcgaccttg
    10021 aaggtggagt gaccggcctc ccggccttcc cgggctaccc ggagggccag ctccccgatc
    10081 tccggcatgg tcttgccgca ggtcagggcc cgggcgtacc ccacgatccc gggcacccgg
    10141 gaaagcacgc gttccgctcg ctcctcgagc ccctcgggca cgcgcaggaa gaaccgaccc
    10201 ggccggatgt ccactcgggc ccggccctcc agcctccgct cgatgtcctt gcggagacgg
    10261 gactcgaatt cccggaggtt gcccgtcttg agctccagct ccccgggctt gatcaggtac
    10321 aaggtatcca tgttgttcct taggtccggt accgcaggta cgcgtcccgg agggcttcgg
    10381 cgaagccgtc gatctcggat tccgtggtag tcgggcccag ggaaacccgg atggcgcaga
    10441 acgccagggc ttccgggact cccatggcct ccagcaccct gcgcttccgg cgggaggagg
    10501 agcaggcgct gccggtggat acgtcgtatc ccgcgtccga aaggatccgc acggcggtct
    10561 ccccggccag gccgggcagg gaaaggccca ggatccaggg ggagtaccgt tcgtcccccg
    10621 ctttcctgca ttcgggcacg ggccggacgt ccgggatggc ccggatgtga tccagtagcc
    10681 ggtccgagag cacccgggcc cgacggcggt tttcccccag gtcccggatc gcggcggcgg
    10741 cagcccgctc gaaggccagg atgcccgcca ggttctccgt gcccgagcgg atgccgcctt
    10801 cctggccgcc ccccgcggcc aggggctcga tcggcgtccg caggtagagg gcccccacgc
    10861 cgcggggccc gcgaatcttg tgggcgctca gggccgcgga gtccgcgccg agggactcgg
    10921 gttcgaaagg gaccttcccg aaggcctgga cggcgtccgt atggaaccgg atccgacgcc
    10981 ctccgcggga ggcctcgtcc agggccaacc ggatttcgga caggggttgg acggccccgg
    11041 tctcgttgtt cacagccatg accgccgcca ggaccgtgtc gggcccgcag gcgtccacca
    11101 ccgcgccggg agcgaccctc ccgtcgggtc cgggatccac ccggcggacc ttccatccca
    11161 ggcgttctaa aaccgcggcc tgctcgaaca ccgcgggatg ctcgatggag gagatcacca
    11221 cggatccggg agacggccgg cggagcaggg cgagcagggg caggccgtcc gcctcggtgc
    11281 ctccggaggt gaagacgacc cgcccgggac ggcatcccag gagtccggcc aggcgcgccc
    11341 gggcgtcctc caaggccgta cgggcggccg aaccctccgc gtgcaggctg gacggatttc
    11401 cggggtactc ccgggcggcc cggaggtatt cctccagggc ttccggggac gggggggccg
    11461 tggcggccca atccagatag gtgcgggaca tcgggttccg gatactgggt ccggccctag
    11521 ccggaactcc ggcggtcggg aatgccctgg acctcccggc ccgccgcggc gaagtccggc
    11581 ctgaggaaca gggcgcagaa gcagtggccg tgctccgcca cgtccgcccg ggcgtagcgg
    11641 cagggacaga ggaccgaggc gtccgcctcc cggctcccct cggtatcccg gcaggggcac
    11701 agatagtagc cgtagcggtt ccagttcgtg gtgagacctt ccaccagggt gcccgtgaat
    11761 tccgcgtccg ggttcaccgt ccagccctgc tttgcggcca ccatgtccgc gaagcgtcgc
    11821 gtatcctcca gggtcttagg agcgggcatc tagacctcca gggtccgctt ccagtccgcc
    11881 tcgatgaagc cgaccaggga ctcccggtcg tccacgaccg cgaaggggaa ggccacgtcc
    11941 tgcttgtacc ggtccttcag gatcttcttg gcctcggtct tcgtctccag ggggaccttg
    12001 tccatataga tgtaggagta ttcgaacttg tgggtatcca ggaaggccat ggcccgcttg
    12061 cagaagccgc aggtggaaag ggcgtacacg gtcaccctgt gctgcttgtt ctccccctcg
    12121 accttcgtat actccagggt atcgagcata gattcctcca tcggcctact atagcgttcc
    12181 gaagcccgct tgtgaaggcg aaaagccccc cggaaccgtc ggcccgtccc agttccgctc
    12241 aggcccaggc cttcgcatag acgtcggcgt cgaacttccg ccggaggccc gcggcgttca
    12301 tgtaccgcac ggtgccgaat acggcgcgtc cggcccaggg ccggtcgtgg cggccgaagc
    12361 accaggccac gccggcgtac ccgttggggt cccggccgtc cagggcgtag cggtcgttca
    12421 ggtacagggc ggtcccgtag gcctcccggg gatccgcgga ccactccagt attttctttc
    12481 cccagtacat gcgcatgtag ttgtggatcg agcccgagcg gaccagctga ttctgggcgg
    12541 cgttccagta ggggtcgtgg gtacgggccg cctcgaaatc cgaccgggaa tagagatagt
    12601 cccgcttgtc ccccgcggcg tcccggaggg tgcggcgggc ccagtcgggc aggccctccc
    12661 agcggtcgta ggcgtcggga agaaagcgga ccaggttcag ggagagctcc cgccggaccg
    12721 tgagctgttc gatgaaggac gcggctccgg ccccgccgcg ctccaggacc tttcgggcca
    12781 ggagcaccgg ggagacctgc ccgaagtgca ggtaggggct catcccgctg gtaccgtcca
    12841 gggtcgggtc gttccggtcc gcgtcgtacc ggtccagccc cgtattcagg aaggtgtcga
    12901 accgtgtccg ggcggcctcc tctcccggtg ggggcagggc cgtctgctcc gggactcgat
    12961 ccccgggata ttcgggccga agcgggccgc cctcggtcct aaggagctcg gcgccgccgt
    13021 ccaggtccag gccccgggcg tcccgccgaa gctccacgac gggcgggatc cgggccgcgt
    13081 aggcgggaac cgcgccctcg atcttccggc gaagggtaaa ggcggaccat tccagcttgt
    13141 cgctggcggc ttccacggga accacggcat ccccgtccac ccggatcacc gggcaggcgg
    13201 accgttccgc gaactcccgc ctccaggcca gctccggccg ggtccaaccc gcgtcgcaga
    13261 ccgccagggc cgcccgccgg gagagctcgg cggctacctt cggggattcc ccccggcgca
    13321 ggatgaagcg ggccccccgg gcctccaggg cccgggcggt ttcggccaag cccccgagga
    13381 ggaaccggta gtgtacggcc gtggcggagg gatagggggc caggacgaag agcaccgcca
    13441 agggcagacg gagccgcgcc gcctcggagg cggcgtactc cagggcggga ttttcccggg
    13501 cccgctgggc gtgctgcatc cagtagagga cgaagtcccc ccgcccgggc ggtccgggct
    13561 tgagcaccga caggcgctcg ggttccacga tcatagggcc agtatacctg cggggcgggg
    13621 ccgcggcata gggccgccgg tcccgggcgg accccggcgc cgccaaccgg agcggtcccc
    13681 cggagcgggg gcgcggtacc cggcgggcga catgagcttg aaggaatcgc cggtttccta
    13741 tagagtgcgc ccatgcgaga cccgaaccat atccggaatt tctgcatcat cgcccacatc
    13801 gaccacggga aatccaccct ggcggaccgc ttcatccaga tggcccacat cgtggacgac
    13861 cggaagttcc agaaccagat gctggacaac atggatatcg aacgggaacg cggcatcacc
    13921 atcaagagcc aggcggtctc catcccgtac accgcctcgg acgggatcac ctacaccctg
    13981 aacctggtgg acacccccgg ccacgtggac ttctcctacg aggtcagccg ggccatctcc
    14041 tcctgcgagg gggccatcct cctcatcgac gcctcccagg gggtggaggc ccagaccctg
    14101 tccaacatgt acatggccct ggagcacgac ctggagatcc tgccggtgat caacaagatc
    14161 gacatgcccg ccgcggacgt gccgggggtc cgggaccaga tctcccacga cctcgggctg
    14221 gacggggacg aggcccttcc cgtgtccgcc aagctgggca ccggcctgcc cgacctcttc
    14281 gaagccatcg tccggcgatt cccgcccccc cggggatccg cggacaagcc cgcccgggcc
    14341 ctgatcttcg actcccggta cgatcccttc cggggggtcc tggtgcatat ccgcgtggtg
    14401 gacggccgga tccagcccgg ggaccggatc ctcctcatga acaccggggc ggtccatgag
    14461 gtggaggaag tcgggttctt ccggatcgag ctttccaaga ccgaggaact gtcagccggg
    14521 gacgtgggct acgtggtggc ggggatcaag acggtttcgg acgtccgggt gggggacacg
    14581 atcacgaacg ccgacgtccc ggcctcggaa cccctgccgg gtttccggga ggtgaagccc
    14641 gtggtctttt cctccatcta ccccatggac acgaacgact acgacgagct caagatcgcc
    14701 atggagaagc tcaagctgaa cgacgcctcc ctggtctacg acaaggacaa ctccgtggcc
    14761 cttggcttcg ggttccgctg cggcttcctg gggctcctgc acctggaggt ggtccaggaa
    14821 cggctggagc gcgagttcga ccaatccata atcatgaccg cccccagcgt tcggtacaag
    14881 atactcctca agaacggcca ggagctcttc gtggacaacc cctcggagta cccggatccc
    14941 ggccgcatcg agaccgccga ggagcccttc atcaaggccc agatcatcac ccccgtggcc
    15001 tacgtgggca acctcatctc cctgtgtgtc cagaagcggg gggtccagac ctccttcacc
    15061 cacctggacg agaagcgggt ggagatgatc ttcgagatgc ccctggcgga ggtcctcttc
    15121 gacttctacg acaagctcaa gagcaccagt cggggctacg cctccttcga ctacgacgtg
    15181 atcggctacc atccctcgga actcgtcaag ctggacatcc tgatcaacgg caagcccgtg
    15241 gacgccctgt cccagctggt cttcaagccc ggcgcctacg agcgggcccg gaaggtctgt
    15301 gagcgcctcc agggggccat ctcccgccag cagttcaaga tcgccatcca gggggccatc
    15361 ggggggacca tcatcgcccg ggagaccgtg aaccctgtgc gcaaggacgt cctggccaag
    15421 tgctacggcg gcgacatcac ccggaagcgc aagctcctgg agaagcagaa ggaaggcaag
    15481 aagcggatga agtccgtggg ggacgtggac ctgccccagg aggccttcct ggccgtgctc
    15541 cggacgaacg aggacgagga ctgatcatcc cagggtgacc ctgcgcccgg tcgctgagga
    15601 gaccttagcg gcctccatca ggcggagggt ttccagggct ccggcggtct cgaccggaag
    15661 gggcccccgg ccggccaggg cggcggccag gccccggtag aactcccgcc agtccccgcc
    15721 ggcgaggtcc atcctctcct cggaaccgtc cgggcgggag agcaggcccg ccccgggcat
    15781 cgtgccccaa ccgggagagc cgggcaggtc tcccgcccga agcacggcct cctgaccgtc
    15841 ctccccgaag gtccggaagg tcgccttcgc cccgtggacc cgatagtgga accccgtatc
    15901 cggggccagg caacccgcgt gcaggatgat cctgcgcttc cccgagccga gcactgcgtg
    15961 gaaccagtcg tccacccggg taccgggtct ctgggccgcc acgtccgcca tcacccagtc
    16021 gaagggcccg aagagccggc acgcctggtc gaagaggtgg ggacccaggt cccacaggag
    16081 accggagccc ggtccgggtc gttccttcca gcggtcctgg acggacgggc ggaaccgctc
    16141 ccagcggaac tcggcctcgg agacctcccc caggacgtcc cggtccagga gcccgctcag
    16201 gctccgaaaa tccgcgtccc agcggcggtt gtggaagacc gccaggggca gtccggcggc
    16261 ttcggatgcc cggatcaggg tctccgcctc ccggacggtc gaggcgaagg gcttttccag
    16321 g
//
LOCUS       DAOTEI010000070        16231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2431_length_16231_cov_5.606825, whole genome shotgun sequence.
ACCESSION   DAOTEI010000070 DAOTEI010000000
VERSION     DAOTEI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 16231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16231
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2431_length_16231_cov_5.606825"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="P5313_08360"
     CDS             <1..247
                     /locus_tag="P5313_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HRY80414.1"
                     /translation="QDRRIDRGSELYKQCQEFESLFVNMMLKEMRKTVDKAGLLDGGQ
                     AEDIFQDMLYDEYAKDMTKAAGFGLSDQVYLQLTGVR"
     gene            380..1045
                     /gene="rsmG"
                     /locus_tag="P5313_08365"
     CDS             380..1045
                     /gene="rsmG"
                     /locus_tag="P5313_08365"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRY80415.1"
                     /translation="MPAVNIDLLSRGLSALGLDPGQDRLARLARYIEEIELWNPAYGL
                     VKASGDELVVKHVLDSLAALPVLRGLGLPLGRVADLGSGAGLPGIPLAIFLPESAVTL
                     VERMERRVRFLENQKARLGLSNVTISQAEAERAPGGPYDLVTFRAFRPFSERKLFLSV
                     RKLVKAGGAIAAYKGRLQTAREELAALAPDPVLGPLAVRAEVLPLSVPFLDEERCLVI
                     LRA"
     gene            complement(1062..2441)
                     /locus_tag="P5313_08370"
     CDS             complement(1062..2441)
                     /locus_tag="P5313_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY80416.1"
                     /translation="MAPVVPILIAVIFVSFAALILGVVLGRGGKRTKTGGRLKARDRA
                     QILKEADRRLASNPKDPDALQALGDLYWGEKAWENVFKTYGVLVEVATGNPEMDEFTF
                     NARYGIAAVYLGKPEEAYKGLLIARTFKQDDFEVNFNLGLIEYQKRQFEKALVLLNQA
                     AQAKPDHAPTLRHLGHAQFKAKRYKEALVNLRKAAELEPEDKDSLFAMGECYYELGQN
                     DQAIRIFSHLRADPKLGPNASLFSGTLQLNMRQFQKAIMDFEIGLRHPEIPVETLVEL
                     KYRLAAAYLRAQELAKAVTVLNEIQSIFPNYKDVPAQLAKYRELNSNRNLQVYLMGAT
                     GEFVTLCRRIALSFFPKAKVKITDIAVNKNDWADILTEVETSKWQDIILFRFIRSTGT
                     VGELTVRDFHARLKDVKAGKGYVVSAGVFSDEARRFVEARLLDLLDKDQLAKLLHTVD
                     SGVRDIISD"
     gene            2809..3927
                     /locus_tag="P5313_08375"
     CDS             2809..3927
                     /locus_tag="P5313_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRY80417.1"
                     /translation="MPIVVGAAVAYALLSWALARLRVHPTATNPLVVNFTATAFGLLL
                     FMALAAARVYFSLGFLTIYILFTNAWFTLEFLLRRRFGMYTFAVVPGGYDLFSQPFPN
                     CRLVPLASPELLPEDLDAVIVDFEQVLPEYWLAFVSRCVMEGVPVISTEDFLETETGT
                     ILLDHLTTARTLAFQKGQVYIALKRLTDTVLIVLAAPLWIPIVLMAMTAVRLESPGRA
                     LYSQMRVGRRGRPFRIFKIRSMRQDAERHGAAFAVKGDARITRLGAFLRKTRIDELPQ
                     FWNVLRGDMSLIGPRPEQVPFVQDFERTIPYYQLRHIVRPGITGWAQVTLGYAANETE
                     TAEKLAADLYYVKRLSFTLDFLIVVKTIWTMLTGFGSR"
     gene            complement(3941..5392)
                     /locus_tag="P5313_08380"
     CDS             complement(3941..5392)
                     /locus_tag="P5313_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80418.1"
                     /translation="MKSKLSSILLTCFLAVMLPFDLSAQNVTTVYRIESVEYRIEGRT
                     REKALADRLEIAIGREFRDLASLEAYVADRRQVLVNQRVLEDAELLYSIGEPVDSVLP
                     VRLTVRARDTWNLIILPYPKYSTNDGLLLSMRLRNYNFLGTMETLYLDLDYRYDESGN
                     QSFGIGTEFTYPFQSAGLDWSVHTEQDFAVSLAGLISYVGQQTLRLHLGEDSFKRTIR
                     VGQKFLWNQYAEDDADLDHLVLHSLTGISTDFRLLSLGGLGYLTLTPDLEAGWKYRVD
                     GTEISPDRKGPYVTGSYSLGAGRVDWSGNLRQGTLVSLYNAYTYNLQSTDWTIVAIAQ
                     ARHHESWFGNRLGLNSRLTLYGSFLGVASDIGGYIRGIPDAYIDADYAVFANLDLPIR
                     LFDFKPSILIKKKWFDFEAHASPFVDAALVRPGEAADFEPYLCAGLELFGFLKQARSI
                     YARLSLGVDALGYLDTRSLSGNYELFFGLGHDY"
     gene            complement(5510..6988)
                     /locus_tag="P5313_08385"
     CDS             complement(5510..6988)
                     /locus_tag="P5313_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80419.1"
                     /translation="MKRSAIFLIIALVATASFAQQVPVPQGADLVDDFGDGSLSLFFD
                     FSGGYDENLIVNGGGGLQYFGENFALTAEAYLRKDDVYSGTSANTATGGMGGLYFWME
                     AGGVAYRAGNLSFRAGRLPHYDYVDSPYSLFINGDGLTANLLEFTYDDGRFLYSSRWM
                     ELNRNSDAITTVYPIGFPDRGSNLHTFAVRFGDMTFGIQDAITYAGKSFDFEYFISPI
                     PAYIIQDARRQGGRPWTETGEENNIIGIFWTWDRTEGWDFHAQWLLDDGNLHWLNPSA
                     FPTNQPYKMAWAGGASKETPYGRFSFYHAGATKYTFEPSYGLPGREYGYAYFPDVVFR
                     LKGSPTDTAIPFETMMLGYKYGENNLAFLGAWNGDLAGFGLGASVEFTISGSKSPANA
                     WHEYNYNPDNGTELLNEDRLEWKVLASLGATRRIWKLDLYAGLALGYVINELELAASG
                     ETAVETQYTDVPYEQLQIWRPSTTSRFLWAATVGGRYRIKIK"
     gene            complement(7108..8082)
                     /locus_tag="P5313_08390"
     CDS             complement(7108..8082)
                     /locus_tag="P5313_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80420.1"
                     /translation="MRNPANPRRLAEAGFDVVMPYAGSFSLDQVREYLSAAEKAGIGV
                     HMDVPRYTYRGAEGNALETFIRTVRDNPSIISWYLADEPEWRPGHSPGLLERAYSRVR
                     ALDAERDIVQVFMFPFRAGAYRGSMDRIWIDWYPVRAGSREHRAFRGGRYADRMKSFG
                     TTADRLGLPLTVVVQGYGEGVDGKPQFGRRLPTPSETRYMFWASFLSRPEEILYWTLY
                     RTRSVWLEESLLPEVRAFRERFPDAVEYVPDPGFRIRGGKADSILLGNGRGDFWLVVL
                     DQEGRDHKIILQAPAGYAFAGGPGIAARETGFHLGPYGVQLLEISEIP"
     gene            complement(8262..9530)
                     /locus_tag="P5313_08395"
     CDS             complement(8262..9530)
                     /locus_tag="P5313_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY80421.1"
                     /translation="MNIWIVCPYAGSVRHGMNYRPYYFGREFARAGHRVVVVSGSYSH
                     QFVRLPETTGTYTRETLDGMEYVWIKVPRYRSSGDPVRTLGWALFCLRVGGVARLGLP
                     RPDAIVVSSPVPYPLVPAARLARRFGARLVFDVRDLWPLSLTALGGYSQRHPFIRLTQ
                     WVEDFACRRADLVVSALPNALEHMSSRGLDPGRFLWVSNGVDPGDAEAAEETDVGSIA
                     GARPVASRDGEFHVCYAGSFHERNVALVFVEAADLLRKRGIGIRFHFLGKDGGGKPAL
                     QARARELGADNVVFHDPIPRSSMQGFLAGMDACLAGTKASPLYRFGISLTKQFDYMLA
                     GKPVVLSSNAVGDIVSRTGCGLVVAPEDPGAAAEAIETLARMDPAAREEMGRRGREAL
                     LAEFTYPKLAARFLEGIQSAVDRGRSGPRG"
     gene            complement(9527..10525)
                     /locus_tag="P5313_08400"
     CDS             complement(9527..10525)
                     /locus_tag="P5313_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103118 -
                     UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine
                     N-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRY80422.1"
                     /translation="MDPVAWPSLAFSGTTAVEAAERFPEDLDLSAPARDLLSDRSFLG
                     PSTAAEPRDGTILFLQAAGPEALGRLAGLRGCLVLLPAGAGPGPLAQENAVLFARNPK
                     YAYALLMTELFPLVLQRGTYSAEPLPGVRADPGFVLDEGVTIEPFVTIGRDVVIGPGT
                     YVMKGAALGPRVRIGRDCLIGEGAVIGDPGFGFAFDGDRPPLRIPQLGGVVIGDRAEI
                     GALSCVDSGTIDATVVEEDVKMNSHVHIAHNCRIGKGSVLGAAVSISGSTRLGERCWL
                     GPGTVVRNKVILGDGVTAGMGAVIVKDVAAHQTVVGLPAKPTGNEVPWYLSEKGTP"
     gene            complement(10513..11145)
                     /locus_tag="P5313_08405"
     CDS             complement(10513..11145)
                     /locus_tag="P5313_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRY80423.1"
                     /translation="MANKRRILVLAPHTDDGELGCGAAIARFVEQGAEVHYVAFSTAK
                     ESVPAGLPENILEVEVREATRRLGILPENLHVFGYTVRKLSYVRQDILEDMVKLRNAL
                     SPDLVLMPSRNDLHQDHSTVASEALRAFKNTSILAYELPWNTIDFSTQGFVVVEPRHV
                     DRKIEALAAYGSQKMRKYMDPEYVRGWARTRGVQIGRDFAEAFEVIRWIL"
     gene            complement(11157..12275)
                     /gene="wecB"
                     /locus_tag="P5313_08410"
     CDS             complement(11157..12275)
                     /gene="wecB"
                     /locus_tag="P5313_08410"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRY80424.1"
                     /translation="MSIVPTLITIIGARPQFIKAAAVSARLASAGIREDILHTGQHYD
                     ANMSGNFFSELGIPEPRWNLESGSGKHGAQTAKMLSGIEEILEAERPSAVLLYGDTTS
                     TLAGALAAVKLGIPVAHIEAGLRRYDRTIPEEVNRRLTDHVSDLLFAPTKTAMENLRK
                     EGLLEGAHLVGDVMLDLALDTRAGETYGLVPLRFGLASRGYVYATIHRQENTDVPEKA
                     RIIWESFCSLAEDGIPIVLPLHPRTRRKLAELGIQVDRRPGRFSVLDPVSYKESISLA
                     GNARFVATDSGGVQRESGFLGVPCVIINYSTGWEEVLSGGGAVLLGKDFSRLKDLLKS
                     AWKNPPTVSDVGKEFGGGKASSRIVEILHDYLETVIQV"
     gene            complement(12335..13441)
                     /locus_tag="P5313_08415"
     CDS             complement(12335..13441)
                     /locus_tag="P5313_08415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY80425.1"
                     /translation="MVRKSVLHISTAHRALDTRIFHKECVSLSRAGFDVTVMARHDRD
                     EKVSGVRIIGLPTDRNRFIRMFFGPFRALYGALGQHVDVAHFHDLELFPVMLALKAVG
                     KKVVFDYHENYTAQVMGKKYLGSDCVRMFIRTIVRRMEKGIDRMADAVVAATDPISFL
                     HDSPLKVTINNFAILETIDRIAPAVVEKKRPVVVYSGALRADRCIREIVGAMNRVGNE
                     AELWLMGAWSSPVFREECLGLSEKGNVKDLGFLDFETMYSFIKAGDVGIALSRPHGNS
                     LEGQPNKLFEYAACGKPIITSDFLYRRKLLGEAAAYVDPLDPESIAYAILDVVRNPER
                     AAATGRRGRLLTEERFSWESEARKLVDLYRTLVN"
     gene            complement(13477..14631)
                     /locus_tag="P5313_08420"
     CDS             complement(13477..14631)
                     /locus_tag="P5313_08420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY80426.1"
                     /translation="MLKLLVVPSRYPNDFNKLQGSFYREQSLALAKLQIDVRVLAVVG
                     YSLKEILKNRKILGTRKEYLDSGVLTVVRSVFNVPKLKRLNYFFRAVLGLSEYNKLCA
                     DWKPDIILAHSFAGGDIAGRISDRTRIPFAITEHSSKFFKKLHFYEALKAQEVFRKAS
                     RRWAVSPALANQLRQTFDLTFETMPNSVDKDMFKPGGRVRSAGDPVVFLNIGNLVPVK
                     RTYELILAFHHARIKCSLPLKLRIVGGGPERKRLERLIVKLEEQAMIELVGTLPRDKV
                     ANIIAESDCLVVASRVETFGVVIIESLSCGVPVISTRCGGPEHILAEAPFCSLVEDVD
                     KSLASKMIEFAERGSVDREAIRTFAVERYSAEAVARRYIEELSSIITQPK"
     gene            complement(14641..15627)
                     /locus_tag="P5313_08425"
                     /pseudo
     CDS             complement(14641..15627)
                     /locus_tag="P5313_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(15635..>16231)
                     /locus_tag="P5313_08430"
     CDS             complement(15635..>16231)
                     /locus_tag="P5313_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HRY80427.1"
                     /translation="GKMDINIWEVIEAAKTKPYGFQAFYPGPGIGGHCIPLDPFYLEY
                     IAKGFNFDLTMINTAGNINNLMPYRMMNKIAFALNRHKKALNGSTILFLGVAYKPDID
                     DPRESPALLVMEAVAKKGARVLYHDPYIPEVTDDHGKTWKGVELTDEILSSADCVVFT
                     TNHACFDVERIVEKAGLVVDTRNAVKRVHIEDGKVFKL"
BASE COUNT         3204 a         5029 c         4816 g         3182 t
ORIGIN      
        1 ccaagaccgc cggatcgacc ggggcagcga actctacaag cagtgccagg agttcgagtc
       61 cctcttcgtg aacatgatgc tcaaggagat gcggaagacc gtggacaagg ccgggctcct
      121 ggacggcggc caggcggagg acatcttcca ggacatgctc tacgacgagt acgccaagga
      181 catgacgaag gcggcgggct tcgggctctc ggaccaggtg tacctgcagt tgacgggagt
      241 ccggtagcag gagcgaggcg acggtccgat cccctgacgg ccgacgaccg atacgaatcc
      301 gccggcgccc ggttgggccc cgagtcctga ctcctgagtc cggattcctg attccccccg
      361 tctctgctac aatcccccca tgcccgcagt caacatcgac ctgctctccc ggggactttc
      421 cgccctgggc ctggaccccg gtcaagaccg cctggcccgc ctcgcccggt acatcgagga
      481 gatcgagctc tggaaccccg cctacggcct cgtgaaggcg tccggggacg agctcgtcgt
      541 caagcacgtc ctggactccc tggccgccct gccggtcctg cgcgggctcg gcctgcccct
      601 cgggcgcgtg gccgacctcg ggagcggggc gggcctcccg ggcatccccc tggcgatctt
      661 cctgcccgaa tccgcggtga ccctggtgga gcgcatggag aggcgggtcc ggttcctgga
      721 gaaccagaag gcccgcctgg gcctttccaa cgtgacgatc tcccaggccg aggcggagcg
      781 ggccccgggc ggcccctacg atctggtcac cttccgggcc ttccggccct tttcggagag
      841 gaagctcttc cttagcgtta ggaagcttgt gaaggctggc ggggcgatcg ccgcgtacaa
      901 gggacggctc cagaccgccc gggaggagct ggcggctctc gccccggatc ccgtcctggg
      961 gcccctcgcg gtccgggccg aggtcctgcc cctgtcggtg cccttcctgg atgaggaacg
     1021 ctgcctggtg attctgcggg cttgatccgg cggaagtacg gtcagtccga gatgatgtcc
     1081 cggaccccgg agtccacggt atgcaagagc ttggccagct ggtccttgtc caggaggtcc
     1141 aggagccggg cctccacgaa gcgccgggcc tcgtcggaga agaccccggc ggagacgacg
     1201 tagcccttcc cggccttgac gtccttcagg cgtgcgtgga agtcccgcac cgtgagctcc
     1261 ccgaccgtgc ccgtggagcg gatgaaccgg aacaggatga tgtcctgcca cttggaggtc
     1321 tccacctcgg tgaggatatc cgcccaatcg ttcttgttca ccgcgatgtc cgtgatcttg
     1381 accttggcct tggggaagaa ggacagggcg atccggcggc acagggtcac gaactccccc
     1441 gtggcgccca tcaggtagac ctgcaggttg cggttggagt tcagctcccg gtacttggcc
     1501 agctgggcgg ggacgtcctt gtagttgggg aagatggact ggatctcgtt caggaccgtc
     1561 accgccttgg ccagctcctg ggcccggagg taggccgcgg cgaggcggta cttgagctcc
     1621 accagggtct ccacggggat ctcgggatgc cgcaggccga tctcgaagtc catgatggcc
     1681 ttctggaact gccgcatgtt cagctggagg gttcccgaga agagggaggc gttgggcccc
     1741 agcttggggt cggcccggag gtgggagaag atccgtatgg cctggtcgtt ctgccccagc
     1801 tcgtagtagc actcccccat ggcgaagagg gagtccttgt cctcgggctc gagttccgcg
     1861 gccttgcgca ggttgaccag ggcttccttg tagcgcttgg ccttgaactg ggcgtgtccc
     1921 aggtgacgca gggtcggggc gtggtccggc ttggcctggg ccgcctggtt caggaggacc
     1981 agtgccttct cgaactgacg cttctggtac tcgatcaacc ccaggttgaa gttgacctcg
     2041 aagtcgtcct gcttgaaggt ccgggcgatg aggagcccct tgtaggcctc ctccggcttg
     2101 cccaggtaca ccgcggcgat cccgtaacgg gcgttgaagg tgaactcgtc catctccggg
     2161 ttgcccgtgg ccacctcgac caggaccccg taggtcttga agacgttctc ccaggccttc
     2221 tccccccagt agaggtcccc cagggcctgg agggcgtccg ggtccttggg gttcgaggcc
     2281 aggcggcggt ccgcctcctt cagtatctgg gcccggtccc gggccttcag gcggcctccc
     2341 gtcttggtcc gcttgcctcc ccggcccagg accaccccca ggatcagggc ggcgaaggag
     2401 acgaagatga ccgctatgag gatgggcacg acgggcgcca tggccccaag tatcggacgt
     2461 ggaaagaagt gttgtcaagg cgcgcgaaga accgcttcgc ggttcttcgc gcggcggccc
     2521 gggcagccgg agacccggga cccggaaacg aaaacggccg ccccggagag cggccgtgcg
     2581 gcagggatgt agattagtat tcatcattga cttagctcat gatagatacg tatgatactt
     2641 cactatatca tgatggattg aggattgttc ttgaaggaaa agctccggaa gcacagcctt
     2701 tccaaactct ttgccgccgc ctggagtgca gtgggcgcaa tcgcacttac ctggatttcc
     2761 gttgtcgagt tccccttcgc ccgggacggc cggatcctgc cgaacgccgt gcctatcgtc
     2821 gtcggagcgg ccgttgcgta cgccttgcta tcctgggccc tggcacggct tcgggtccac
     2881 cccaccgcga cgaatcccct ggtcgtgaac ttcactgcca cggccttcgg actcctcttg
     2941 ttcatggcgt tggcggcggc acgggtctat tttagcctgg gctttctgac gatatacatc
     3001 ctttttacca acgcctggtt cacactggaa ttcctcctga gacgaagatt cggaatgtat
     3061 acctttgccg tggtaccggg gggatatgac ctcttcagcc aacctttccc caactgccgg
     3121 ctcgtccccc tggcttcccc ggaactccta ccggaggacc tggacgccgt gatcgtagat
     3181 ttcgagcagg tgctcccgga atactggctg gccttcgtgt cccgctgcgt gatggagggc
     3241 gtgcccgtca tttccacgga ggacttcctg gaaaccgaga cggggaccat cctcctggac
     3301 catctgacca cggcccgcac cctggccttc cagaagggac aagtctatat cgccctcaag
     3361 cgcctcaccg acaccgtcct gatcgtcctg gccgcgcccc tctggatccc gattgtcctc
     3421 atggccatga cggccgtacg gctggaatcc cctggccgag ccctctactc ccagatgcgt
     3481 gtgggtcgcc ggggccgtcc cttccgcatc ttcaagatcc ggtcaatgcg gcaggacgcg
     3541 gaaaggcacg gagccgcctt cgccgtcaag ggggatgccc ggatcacccg actaggcgcc
     3601 ttcctccgga aaacgcggat cgacgaactg ccccagttct ggaacgtcct gcggggggac
     3661 atgagcctta tcggcccccg tcccgaacag gtgcccttcg tccaggactt cgagcggacc
     3721 atcccttact accagctccg gcacatcgtc cggcccggaa tcaccggctg ggcccaggtg
     3781 acactgggat acgcggccaa cgaaacggag accgcggaaa agctggccgc ggacctctac
     3841 tacgtgaagc gcctctcctt taccctggac ttcctgatcg tcgttaagac gatctggact
     3901 atgctgaccg gcttcggctc ccgatgatcg gtcatcgggc tcagtagtcg tgtcccagcc
     3961 cgaagaagag ctcgtagttg cccgataggc tccgggtgtc caggtagccc agtgcgtcta
     4021 cgcccaggga caatcgggcg tagatggacc gcgcctgttt caggaaacca aaaagctcta
     4081 acccagcgca gaggtatggc tcgaaatccg ctgcctcccc gggccgtacc agagcggcgt
     4141 ctacaaaggg cgaggcgtgg gcctcgaaat cgaaccactt tttcttgatc aggatagaag
     4201 gcttgaagtc gaagagccgg atcgggagat ccaagttcgc gaataccgcg tagtcggcgt
     4261 cgatatacgc gtcggggatt ccccggatat agcctccgat atcggaggcc acccccagaa
     4321 aactaccgta cagggtcaaa cgggaattca ggcccaggcg gttaccgaac catgactcgt
     4381 ggtgccttgc ctgggctatg gccacaatag tccagtccgt actttgcaga ttgtaagtgt
     4441 aggcgttgta tagactcacc aaagtccctt gccggagatt cccggaccag tccacacgac
     4501 cagcaccgag cgaataggat ccggtgacat aggggccctt ccggtccggg gaaatctccg
     4561 taccgtccac ccggtacttc cagccggcct ccaggtccgg ggtaagggtg agatacccaa
     4621 ggccgcccag tgaaagcagt ctaaaatccg tgctgatacc cgtcaaagaa tgcaaaacca
     4681 ggtggtcaag gtcggcgtcg tcttccgcgt attggttcca gagaaacttc tgtccgaccc
     4741 gaatcgtccg tttgaaagaa tcctccccga gatggagcct cagggtctgc tgaccaacat
     4801 aggaaatcag gccagccagg gataccgcaa aatcctgttc ggtgtggacg ctccagtcca
     4861 atccggccga ctgaaagggg tatgtaaact ctgtcccgat cccgaaggac tggttcccgc
     4921 tctcgtcgta gcggtaatcc aagtccagat acagcgtctc catggtgccc aggaagttgt
     4981 agttccgtag gcgcatgctt agaagaagcc cgtcgttcgt gctgtacttt ggatagggaa
     5041 ggatgatcag gttccaggta tcccgggcgc gtactgtcaa tcgaaccggc agtacgctgt
     5101 cgaccggttc accgatcgaa tacagaagtt cagcgtcctc cagaacacgc tggttcacca
     5161 gcacctgcct gcgatccgcg acataggcct ccagagatgc caggtcccgg aattcccgcc
     5221 cgatcgcgat ctccaacctg tccgccaacg ctttctcccg ggtccggcct tcgatccggt
     5281 attccacgga ttcgattcgg tagacggtgg taacattctg agcggacaag tcgaaaggga
     5341 gcatcacggc aagaaaacag gttagaagaa tagaggacag cttggatttc atcaatactt
     5401 ctccaggatt tggtacggcg ccaccggacg cctaggaata ctgcttgatt atatgaaaac
     5461 ggcgcgcccc gggatccccg gcaacgcgcc gtttaatacc gaagaacagc tacttgatct
     5521 tgatgcggta gcggccgccc accgtggcgg cccagaggaa gcggcttgtc gtggaggggc
     5581 gccagatctg gagctgttca tacggaacgt cggtgtactg cgtctcgact gctgtctctc
     5641 ccgaggccgc tagctccagc tcgttgatca cgtaccccag ggctagaccc gcgtataggt
     5701 ctagcttcca aatcctccgg gtggccccga gggaagccag gaccttccac tccaggcggt
     5761 cttcattcaa caactccgta ccgttgtcgg ggttgtaatt gtactcgtgc caggcattgg
     5821 cgggactctt ggacccggag atcgtgaatt ccacggaagc tcccaaaccg aagcccgcta
     5881 gatccccgtt ccaggccccc aggaaggcca ggttgttttc cccgtacttg tagcccagca
     5941 tcatggtctc gaaggggatg gccgtgtccg tgggggagcc cttgaggcgg aagaccacgt
     6001 ccgggaaata ggcgtagccg tattcccggc cgggtagccc ataggaaggt tcgaaggtgt
     6061 acttggtcgc cccggcatga tagaaggaga aacgcccgta gggggtctcc ttggaagcgc
     6121 cccctgccca ggccatcttg tagggctggt tggtcgggaa agccgaaggg ttcaaccagt
     6181 ggaggttgcc gtcgtccaaa agccactggg catgaaaatc ccagccctcc gtccggtccc
     6241 aggtccagaa gatcccgatg atgttgttct cctccccggt ctcggtccag ggccggccgc
     6301 cctggcgccg ggcgtcctgg atgatgtagg cggggatggg ggagatgaag tactcgaaat
     6361 cgaaggattt tccggcgtaa gtgatggcat cctgaattcc gaaggtcatg tccccgaagc
     6421 ggactgcgaa ggtgtggagg ttcgagcccc gatccgggaa gcctatcggg tatacggtcg
     6481 ttatagcatc cgagttccgg ttcagctcca tccaccggga ggagtagagg aaacgcccgt
     6541 cgtcgtacgt gaactccagg aggttggccg taaggccatc cccgttgatg aagagagagt
     6601 acggggagtc gacgtagtcg tagtgaggca ggcggccggc ccggaaggag aggttccccg
     6661 cccgataggc gacccccccg gcttccatcc agaagtagag accccccatt ccgcccgtgg
     6721 cggtgttggc gctggtcccg gagtagacgt cgtccttgcg gaggtaggcc tccgcggtca
     6781 gggcgaagtt ctccccgaag tactgcaggc ccccgccgcc gttcacgatg aggttctcgt
     6841 cgtacccgcc ggagaagtcg aagaagaggg agaggcttcc gtccccgaag tcgtcgacca
     6901 ggtccgcccc ctggggcacc gggacctgct gggcgaagga cgcggtcgcc acaagagcta
     6961 tgatcaggaa aattgcggat cgtttcaaat caggacctcc gaatttcgta atccggtcta
     7021 ccagctcggg tttgcccggg gccgtgcagt ccggccgcaa acctgggtat tgtaccgtca
     7081 aagccccggc gcggataggc gggagattca gggtatctcc gatatctcca gtagttggac
     7141 cccgtagggt ccaagatgga aaccggtttc ccgggctgct atcccggggc cgcccgcaaa
     7201 ggcgtatccc gccggggctt ggaggatgat tttatgatcc ctcccctcct ggtccaagac
     7261 caccaaccag aaatcccctc ggccgttccc caggaggatg gagtcggcct tgccgccccg
     7321 gatccggaag cccgggtccg gaacgtactc gaccgcgtcg gggaagcgct cccggaaggc
     7381 ccgcacctcg ggaaggaggg actcctccag ccagacggac cgggttcggt agagcgtcca
     7441 gtagaggatc tcctcggggc gggacaggaa ggaggcccag aacatgtacc gggtctcgct
     7501 cggtgtcggc aggcgccggc cgaactgggg ctttccgtcc accccctcgc catagccctg
     7561 aacaacgacc gtcaacggca gacccagtcg atccgccgtg gttccgaacg acttcatccg
     7621 atccgcgtac cgaccacccc ggaacgcccg gtgttcgcgg gatccggcgc gaacgggata
     7681 ccaatcgatc cagatcctgt ccatggaccc ccggtacgct cctgcccgaa aggggaacat
     7741 gaagacttgt acgatatccc gttcggcatc cagggcccgc accctggaat atgcccgttc
     7801 tagtagtcct gggctgtgcc ccggcctcca ttccggttca tcggccagat accaggagat
     7861 gatggaagga ttgtcccgca cggtccggat gaacgtctcc agggcgttcc cctcggcccc
     7921 gcggtaggtg tagcggggca cgtccatatg cactccgata cccgcctttt ccgcggccga
     7981 gaggtactcc cggacctggt cgagggaaaa actcccggcg tacggcatca ccacgtcgaa
     8041 tcccgcttcc gcaagcctcc tcggattcgc ggggttccgc acgctatcgt accatccgat
     8101 gcggaaagcc tccacgaagt acgatttccc gggtcccggc cgagggttga ccgagcccgg
     8161 acggggggct tgggcggggc ccggggaggc gcacgacagg aacaggaccg aggcaaggaa
     8221 aaccgccgca gtcgagacgg aaatcgcgcc gagagaagcc ctcatccccg aggaccggac
     8281 cgtccacggt caaccgccga ttgaatcccc tccaggaacc gggccgcgag cttggggtag
     8341 gtgaactccg ccagcagggc ctcccggccc cggcgtccca tctcctcccg ggcggcggga
     8401 tccatccggg ccagggtctc gatcgcctcc gcggcggctc cgggatcctc cggggccacg
     8461 accagcccgc agcctgtgcg gctgacgatg tcccccacgg cgttggagga caggacgacg
     8521 ggcttgcccg ccagcatgta gtcgaactgc ttggtcaaag agatcccgaa gcggtacagg
     8581 gggctggcct tggtgcccgc caggcaggcg tccatcccgg ccaggaagcc ctgcatggag
     8641 gacctcggga tgggatcgtg gaagaccacg ttgtccgccc cgagttcccg agcccgggcc
     8701 tggagggcgg gcttgccgcc cccgtccttc cctaggaagt ggaaccggat gccgataccc
     8761 cgcttccgaa gcaggtctgc ggcctccacg aagaccaggg ctacgttacg ctcgtggaag
     8821 gagcccgcgt agcagacgtg gaactcgccg tctcgggagg caacggggcg tgcgccggcg
     8881 atgctcccga catcggtttc ttccgcggct tccgcgtccc cggggtccac cccgttggac
     8941 acccagagga aacggcccgg atccaggcct cgggaggaca tgtgctccag ggcgttcggg
     9001 agggcggaga ccaccaggtc ggccctgcgg caggcgaaat cctcgaccca ttgggtcagg
     9061 cggatgaagg gatgccgctg cgaatacccg cccagggcgg tgaggctgag gggccaaagg
     9121 tcccggacgt cgaacacgag cctcgcgccg aagcggcggg ccaggcgagc ggcgggcact
     9181 aaggggtacg gcacgggcga ggacacgacg atcgcgtccg gcctcggcag gcccaggcga
     9241 gcgacaccgc ccacgcgtag gcagaatagg gcccagccca aggtccggac aggatccccg
     9301 gatgagcggt accggggcac cttgatccac acgtattcca tgccgtccag ggtttccctg
     9361 gtgtaggtcc ccgtcgtctc cggaaggcgc acgaactggt gggagtacga gccggacacc
     9421 acgacgaccc ggtgccccgc ccgggcgaac tcccggccga agtagtaggg gcggtaattc
     9481 atcccatgcc ggacggaccc cgcgtagggg cagacgatcc agatgttcac ggcgtcccct
     9541 tctcggatag ataccaggga acctcgttcc ccgtgggctt ggcgggaagg ccgaccaccg
     9601 tctgatgggc ggccacgtcc ttgacaatta cggcacccat ccccgcggtc accccgtcgc
     9661 cgaggatgac cttgttcctc accacggttc cgggccccaa ccaacatcgc tctccgaggc
     9721 gggtgcttcc ggagatgctg acggcggctc cgaggacgga tcccttcccg atcctgcagt
     9781 tgtgggctat gtggacgtgg ctgttcatct tgacgtcctc ttccactacc gtagcgtcga
     9841 tggttcccga atccacgcag gaaagagccc cgatctccgc ccgatccccg atcaccacgc
     9901 cccccaactg ggggatccgc aggggaggcc ggtccccgtc gaaggcgaag ccgaagcccg
     9961 gatccccgat caccgcgccc tcgccgatca agcagtcccg tccgatccgg acccggggtc
    10021 cgagggccgc acccttcatg acgtacgttc ctggaccgat gacgacgtcc cggccgatcg
    10081 tgacgaaggg ctcgatcgtc acgccctcgt ccagaacaaa gcccgggtcc gcccggacac
    10141 ccgggagtgg ttccgccgaa tacgtccccc gctggaggac cagggggaaa agctcggtca
    10201 tgagaagagc ataggcgtac ttcggattgc gggcgaagag gaccgcgttc tcctgagcca
    10261 gggggcccgg acccgctccg gccggcagga ggaccaggca gccccgcagg ccggccagac
    10321 gtccgagggc ctccggtccg gcggcctgca ggaaaagtat cgtcccgtcc cggggctccg
    10381 ccgcggtgga ggggccgagg aatgaccggt ccgacagcaa gtcccgggct ggcgcggaaa
    10441 gatccaggtc ctcggggaac cgttccgccg cctcgacggc cgtcgtgccg gagaaggcca
    10501 ggctcggcca ggctacagga tccatcggat cacctcgaaa gcctccgcga agtcacggcc
    10561 gatctgcacc ccccgggtcc gagcccagcc ccggacatat tcgggatcca tgtacttccg
    10621 catcttctgg gacccgtagg ccgccagggc ctcgatcttg cggtccacat gccggggctc
    10681 cacgaccacg aagccctggg tcgagaagtc gatggtgttc cagggaagct cataggccag
    10741 gatggaggtg ttcttgaaag cccgcagcgc ctcggaggcc accgtggaat ggtcctgatg
    10801 caggtcgttc cgggagggca tgagcaccaa gtccggggac agggcgttcc ggagtttgac
    10861 catgtcctcc aggatgtcct gccggacgta gctcagcttc cgaaccgtgt acccgaagac
    10921 gtgaaggttc tccggtagga tcccgagcct ccgggtggcc tcccggacct cgacctccag
    10981 gatgttctcc ggaagccccg cgggcacgga ctccttggcc gtggagaagg ccacgtagtg
    11041 gacctcggcc ccctgctcca cgaaccgggc gatggcggcc ccgcagccca gctccccgtc
    11101 gtccgtatgg ggggccagga cgaggatgcg gcgtttgttt gccatgagga tccttcctaa
    11161 acctggatca cggtctcaag ataatcatga aggatttcga cgatccggga tgaggccttc
    11221 cccccgccga attctttacc cacgtccgac accgtcggag gattcttcca tgccgattta
    11281 aggaggtctt tcagccgaga aaaatctttt cctaaaagta cggcgcctcc acccgacaag
    11341 acttcttccc aacctgtgga gtagttgatg atcacgcagg ggacacccag gaagccgctt
    11401 tctctctgga ctccgccgga atccgtcgcc acaaatctag cattcccggc cagggagatg
    11461 gactccttgt aggataccgg atccagtacg gaaaaccttc caggtctcct atccacttgg
    11521 attcccagtt cggcgagctt tcttcgggtt ctgggatgga gaggcaatac gatcggaatc
    11581 ccatcctccg ccaaggagca aaacgattcc catatgattc gggctttttc agggacatcc
    11641 gtattttcct gacggtggat cgtagcgtac acataaccgc gagacgcaag tccgaaccgg
    11701 agagggacca ggccgtaggt ctcccctgcc ctggtatcca gtgcgagatc tagcatcacg
    11761 tcccccacca ggtgggctcc ttctaggagc ccttctttcc tgaggttttc cattgcggtc
    11821 ttcgtgggcg cgaataaaag atcagaaaca tggtccgtga gtctccgatt tacctcttct
    11881 gggatcgtcc ggtcatacct tcgaagtccc gcctcgatat gtgcgacggg aatccccagc
    11941 ttgaccgcgg ccagggcccc cgcaagggta gaagtagtgt ccccgtacag gaggaccgcg
    12001 gaaggacgtt ccgcctccag gatctcctcg atcccggaca gcatcttggc cgtctgggcg
    12061 ccgtgctttc cggagcccga ctcaagattc caccggggtt cgggaatccc taactcggag
    12121 aaaaagttcc cggacatatt cgcgtcgtag tgctgtcccg tgtggagtat gtcttcacgg
    12181 attcccgcgg aggcaagacg ggccgagacc gcggcggcct taatgaactg aggacgggca
    12241 ccgattatcg tgatcaatgt agggacgatg gacaacgtaa aatcctttca tatagtcgat
    12301 tagcacgtct caatgatcga tgattcgaat aatatcagtt aaccagcgtt cgataaagat
    12361 cgactaattt cctcgcctct gattcccaag agaaccgttc ctccgtcaga agcctcccgc
    12421 ggcgacccgt tgctgccgcc ctttccgggt ttcggacaac gtccaggata gcgtaggcta
    12481 tcgattcagg atccaaggga tccacatatg ccgcggcctc gcctaaaagt tttctgcggt
    12541 acagaaaatc ggaagtaatg atcggttttc cgcaagcggc atactcaaac aatttgttcg
    12601 gctggccttc caaggaattc ccgtggggcc gggacagagc gatcccgaca tcgccggcct
    12661 taatgaagga gtacatcgtc tcgaaatcca ggaaaccaag atccttgaca ttcccctttt
    12721 ccgacagacc cagacactct tccctgaaaa ccggggaact ccatgctccc atcagccaaa
    12781 gttccgcctc attcccgacc cggttcatag ccccgacgat ttcacggatg cagcgatccg
    12841 ctcggagcgc gccggagtac accacgacgg gcctcttttt ttcgactacc gccggggcga
    12901 tccggtcgat cgtctccagg attgcgaaat tgttgatcgt gaccttcaag ggtgagtcat
    12961 gaaggaagct gatcggatcc gtcgcggcca ctacggcgtc cgccattcga tcgatgccct
    13021 tctccatccg gcgcacgatg gttcggatga acatccggac acaatccgaa cccaagtatt
    13081 tttttcccat cacctgggct gtgtagttct catggtaatc aaacaccacc ttcttgccga
    13141 cggccttcag ggccagcatc acgggaaaca actcaaggtc gtggaagtgc gccacgtcca
    13201 catgctgtcc taaagctcca tataacgccc ggaacggacc aaaaaacata cggataaatc
    13261 ggttccggtc cgtgggtaga ccgatgatcc gcacaccgga gaccttctcg tccctgtcgt
    13321 gccgggccat tacggtgacg tcgaatcctg cccgggacag agagacgcat tccttatgga
    13381 agatcctcgt gtcaagggca cggtgagccg tggagatgtg aaggacactt ttcctgacca
    13441 tatcgctcct tctcgtacaa gcttcctaga gtgcaattat ttcggttggg tgataatact
    13501 agacaattcc tcaatatatc ttcttgccac cgcttccgcg gaataccgtt cgacggcgaa
    13561 tgtccggatc gcttcccggt ccacggatcc tctctccgcg aattcgatca tcttggaggc
    13621 caaggattta tcgacatctt ccaccagact acagaacggg gcctcggcca gaatatgctc
    13681 cggtccgccg caccgggtcg aaatgacagg tactccgcag gacagagatt ctataatcac
    13741 aacaccgaag gtctcgactc ggctggcgac gacgagacaa tcggattcag cgataatgtt
    13801 ggccaccttg tcgcgaggca gcgtccctac aagctcgatc atcgcttgct cctcaagctt
    13861 gacgatcagc ctttccaacc gctttctttc gggaccaccg cccacgatac gcagcttcaa
    13921 cggcagagaa cacttgatcc gggcatgatg gaacgccaag atcaactcat aggtgcgttt
    13981 tacggggaca aggtttccga tgttcaagaa aacgacaggg tcacctgcac tacggactct
    14041 tccaccgggc ttgaacatat ctttgtccac cgaattgggc atagtctcga acgtaagatc
    14101 gaaggtttgc cgaagttggt tcgccagggc gggactcacc gcccaacggc gagaagcctt
    14161 tcgaaatacc tcttgagctt tgagcgcttc gtagaaatgc agcttcttga aaaacttcga
    14221 agagtgttcc gtgatagcga aaggtatccg ggtacgatct gaaatccttc ccgcgatatc
    14281 acccccggca aaggaatgtg caagtatgat atccggtttc caatcagcac aaagtttatt
    14341 gtattccgag agaccgagca cggctcgaaa gaaataattc aaacgcttca gctttggcac
    14401 attgaagaca cttcggacga cggtcaaaac tccggaatcc aggtattctt tccgtgttcc
    14461 gagtattttc ctgtttttca ggatttcctt caaggagtac cctacaacgg ccaggactct
    14521 aacatcaatt tgaagtttcg cgagagccaa ggattgttcg cgataaaaag acccttgcag
    14581 cttgttgaag tcgttgggat atcgtgatgg aactacaagc agcttgagca cagtcgcccc
    14641 ctaatcgata aatcgaaagc atcttctaaa tcgaagttgc agtttcttga acgtcttctc
    14701 gagcttgacc atgccttttt tgaaaaccca tgaagaaaaa agcatcggaa gccaaagata
    14761 aaacatacta aaacgaaacg atccacatcc tgtttccatt gccggtagca ctttggctct
    14821 tatatagttc gcggcgagaa tgtgcccctg tagcaaataa tgaaggtata gctttgcgac
    14881 tgcttttatc ctgttctttt ggttggttag ccacatatcc ttgggcaggc tgatcaatgt
    14941 ccgtagcaag agaaaatccg cttctttctt gtgtagatct tttttcgtct ttgagtcttg
    15001 gttcggatgt ctgcgggatt gatccgaggc gattggacaa tacatgaacg aatatccttt
    15061 ttttaacatc ctgaaccaaa gatcatagtc ctggacagtt ttcaggcttt cgttgaacca
    15121 tccaacgctc gagaatgcgt ggctcggaat caacagggaa caaccgccga tgaatctttt
    15181 ataaagcaac gtatacggca acggttcggc gctcttcttg aagggccggg tccgtttaat
    15241 gattctgccc tggccgtcaa tttgatcttc gtgggaataa atgatcgcct tttcgatgcc
    15301 cagcgtatag tagaacgcga cctgtttctc gatcttgtcc ggataatata gatcgtcatg
    15361 gctcaaccag gagaagaatt tttcctgcat ccgctctatc ccgaaattca aagcggacgc
    15421 taccccccca ttcttcttct cgaaatatct gacttcctcg aacccctacg cgattcgccg
    15481 ggtagcccca tcgtccgtgg agccgtcatt gatcacaagg atctccactt cccggtaggt
    15541 ctgatttacg acgctctcga tggcttccga aagataattc gatccgttgt agaccggtat
    15601 gattatggat actaaatcgt attccatgta atcctcacag cttgaacacc ttcccgtcct
    15661 cgatgtgcac ccgcttgacc gcgtttcggg tatccaccac caacccggcc ttctccacga
    15721 tgcgctcaac atcgaagcag gcatgattcg tggtgaagac cacgcagtcc gcggaggaca
    15781 ggatttcgtc cgtcaactcc acgcccttcc aggtcttacc gtggtcgtcg gtcacctcgg
    15841 gtatgtacgg gtcgtggtag aggaccctgg cgcccttctt ggccaccgcc tccatcacca
    15901 ggagagccgg actctcccgg ggatcgtcga tgtccggctt gtaggccacc cccaggaaaa
    15961 gaatcgtgga gccgttcagg gcttttttgt gccggttcag ggcgaaggcg atcttgttca
    16021 tcatccggta gggcatgagg ttgttgatat tgccggccgt gttgatcatg gtcagatcga
    16081 agttgaagcc cttggcgatg tactccaggt agaaggggtc cagggggatg cagtggccgc
    16141 cgatcccggg accgggatag aaggcctgga acccgtaggg cttggtcttg gccgcctcga
    16201 tgacctccca gatgttgatg tccatcttgc c
//
LOCUS       DAOTEI010000071        16129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2455_length_16129_cov_5.679607, whole genome shotgun sequence.
ACCESSION   DAOTEI010000071 DAOTEI010000000
VERSION     DAOTEI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 16129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16129
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2455_length_16129_cov_5.679607"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="P5313_08435"
     CDS             <1..331
                     /locus_tag="P5313_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HRY80428.1"
                     /translation="FSGDLYDFEPTLPSACPGDLGLSVPAKVLRSVWNALKLLDTIVP
                     GVLHPSTILYYPEIKLYANRPVFRDDSFQAAPGIRLVGDGAGTSRGITGAWASGIRAA
                     DGILAGC"
     gene            445..1542
                     /locus_tag="P5313_08440"
     CDS             445..1542
                     /locus_tag="P5313_08440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY80429.1"
                     /translation="MDESADPREDPPDPAAAVSAPRLPNRGACLSASALAGSSALAVY
                     AFLAQEGAARTAFGLLHSLQTLFWLGGLAVFLLRPSYNRRTPYARFSGIAGGVLLGIS
                     LAYGAAAAWTGAGFPIWAAFAVAGLSLARLPRGYRGAYALLAGTALSLLSFYLRGVQA
                     LRADLVYLLAPLALGHAAGSFVDRIRTEADLSLKDLKSQNEELEELAYRDPLTGLYNR
                     RFGLESLGNAISFAQRYKQELHIFLLDLDHFKRVNDELGHPAGDRVLVQLAQLLRKAL
                     RESDIVARIGGEEFLVILPVSRAELVHGAANRIRDITARTTFEPVPWQVTVSIGVTGL
                     REGDTPESMLSRADRFLYDSKRGGRNRVTGS"
     gene            complement(1620..2951)
                     /locus_tag="P5313_08445"
     CDS             complement(1620..2951)
                     /locus_tag="P5313_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HRY80430.1"
                     /translation="MASIPVLVKILVVFGIVVAAASRKLHLGLAALAGGWAFALWQGL
                     PFGRVVLRSLQEFLAAENLLLLLLVTLIMVLSGAMRKAGAMEDFARSVKAVARSRNAS
                     IILTPVLIGTLPMPGGAAFSAPLVEALDPEGRLGADRLAAANYWFRHSLEVWWPLFPS
                     FILTATLTGFEVSRLVLINFYMFPTMVFLGWLFLLKLPGRGAAGTPSPERTGPGSAAA
                     PRTGAGEVIAGFAPLILILGVYAALDLAWRALGPSLGLPGPVSALAGRYFPILTGIGA
                     GMAYVAVRNRGRAVWKDTFNPGTLKLAGVVAGIRVFSALLSEGGAAQSAASELAAAGI
                     PPLAVAALLPLISGLVTGVGFGYVGLSLPIVLGLFRAGSFGSLALDPIVALVAAFGFA
                     GMMVSPLHVCMVVSAEHFKATLPRTIRRFAAPLGLFLVAAVAYVAILTRIL"
     gene            complement(2965..3360)
                     /locus_tag="P5313_08450"
     CDS             complement(2965..3360)
                     /locus_tag="P5313_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRY80431.1"
                     /translation="MEGIERSRKRSRQREEILAVLRSTKEHPTAAMLHTAARRRIPRL
                     SLGTVYRNLDVLEEMGLVLHIRNGNAQDRFDGDVSPHYHFVCDACGAVLDMPLPYRPE
                     LDEAAAREGFRVRGHRLDFHGTCPACSKP"
     gene            3624..5714
                     /locus_tag="P5313_08455"
     CDS             3624..5714
                     /locus_tag="P5313_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY80432.1"
                     /translation="METRAVLPAACLAVLLTAACGVRGPVGQAPAEPPRAVRGQLDLS
                     ASKEGVVALDGPWAFWWGRFLDPPDLADGSEAAPDGFGEVPGAWNGYGIPEAVRQGHG
                     TYRLTVRGLDPAVRYALRLSSFNTAARVFANGRVVHLQGMPGGSRDLEEPDWTAVVVP
                     ADPDSRGVLDIVLHVSNYADRTGGFVNRAFLGPYEVVDTLRDRERMTEVFLFGGILLM
                     GFYYLALFFFRRKDRASLWFGFLCIVLAFRILCYDEYLIRELLPGLPWVWLFRAGYLT
                     FSLAVFLIAAFLRSLFPASWWKPLSWVIYTGAAAYSLVVAVFPPLVSGNLLTAFQVFA
                     LASGAALFAVLLRALLSREPGAALLAGGFGCLFVSVVHDVLASQGILPGPFVVQVGLL
                     FFLFAMSLVVTRKFAVSFETSERLAESLRRMNLSLERFVPREFLRYLNKSSLDEISLG
                     DHSAQRMTVLFSDIRSFSRISERLSPEETFRFINSYLERVVPSIRSHGGFVDKYLGDG
                     VMALFPQDADAAVRCAVDMQRRISSFNQEQAGKWLEPVSAGVGIHSGLLMLGTIGENE
                     RMDGTVISDAVNLASRLEGLTKEYEIGIAVSEEIVEALTDRSAWKTRYLGKIGVKGKR
                     EHVSVFEVYDGDPEDLIVRKDSIRGTFEEAIAAFYRQDFPSAMAGFRSVLEILPRDEA
                     SHYYIRMIRKMNLS"
     gene            5888..6922
                     /locus_tag="P5313_08460"
     CDS             5888..6922
                     /locus_tag="P5313_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY80433.1"
                     /translation="MKKTAIALALSMAILALAGCGGGKAEKKVLYIYNWTYYIPDDVV
                     EDFEKEFNCEVVYDVFSSNEDMFAKIKAGGSGYDVVFPGADFVSIMIKEGMLEPLDPK
                     KIPNLKNLDPAVTSRMSFDPGNKYSVPYFMGATGIVVNKKIAGNFERSWNIYERRDLK
                     NKMTLLDDPRETIGAALKYLGYSVNSVNPDELAKAEAQIKKWLENVLRFDSESFGKGY
                     ATGEYAVVHGWAENVFLELDDALKADAEFFIAKEGGVAYMDCMAILKGAKNRDLAHAF
                     INYIQRDDIYARIASYLALPSVNVPARAKLTEKPNYTEADLARCELKLDIGDSITLYS
                     DLWKRLRLGD"
     gene            7106..7744
                     /locus_tag="P5313_08465"
     CDS             7106..7744
                     /locus_tag="P5313_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80434.1"
                     /translation="MKNLWIVLAAAAAFAAPAFPQEPDPFAGLDSIAPALDRPGDYRG
                     GDVFIRASARRDRLVVVEIPIDGRGNAGRYEVEAHNRVTGGVSADLITRDGKVPGYAA
                     VPEGFPEGTHRVTSVSARTDKFGPYSIGTDAEGLVDAYKVTKQDGKIVSSEYAGKVAD
                     YGYSIHSNREKSFETSRSWGCIIVREADCGRIARSIRADRAAGGDQYVVAGR"
     gene            7872..8777
                     /locus_tag="P5313_08470"
     CDS             7872..8777
                     /locus_tag="P5313_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80435.1"
                     /translation="MAHKGFRMTAARIGRIALLTAGLVAGLAAGLAACGPGASERAPA
                     EAKAPSGTSGAPAAAPSTGAGPASGPEAAVFEYRYTSVRIEDPGAVRYRAEFRFHPDG
                     TIRRAELREIRSGTETLRASAVSEPLQGGYKVAYRSFGRDPRGYDYTLGWSETGLTVT
                     AGGEATGFRFDPAGRVFTVPWDKGAETYSPGPDGSVLGELVLAGERQWGGRLFKSGGS
                     LRFEDRYPDGSVDRECVLKAAGPDAWSVSCTGSADSFYEGSLTGASLFPRGGVYPASA
                     YNLIIVTDLWMTEERFPFFCLSLLE"
     gene            8782..9360
                     /locus_tag="P5313_08475"
     CDS             8782..9360
                     /locus_tag="P5313_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HRY80436.1"
                     /translation="MEALFLAWMIRAAVGREALRFEAVHEQEGDLSCGLAAAASLADL
                     YWGLPCREADLRSALAPEDGRVERATDLGELAALLSARGFAVRAVRLDAVSAAEALRR
                     GYVPLLVHLRDDGGHFALLLALERDVVVLADPARGLFTLDTGEFAASFSGGALAVVFP
                     GVRVDRDRLDARTAGALGARRFLAGLAVGAFR"
     gene            9357..10211
                     /locus_tag="P5313_08480"
     CDS             9357..10211
                     /locus_tag="P5313_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80437.1"
                     /translation="MKTPAPAAALAAAVAALTVSVPPAACSEAPRARTGSEEPPALSF
                     LAEVRPIPRDLPVGRVLDADWEFGLDLRPPGILRFSIRLPLSVRIGQEGPWAGRSDAA
                     WGDPAVEAGAFFRKGAGFRFLGAGYAAPLGRWMPDPARPLRPAPGSGAHRATLSAGLG
                     RIRDPAALSAGVSWTVSIPRPDIPGAAWRPADLSLSLSLVEVLNDRAGILLGLSPRLR
                     APARGPGADPESGFDWALEARVEVHLRTGGAFFRTGVSRSLTEPGAVWVPVAGVEYEV
                     RIRTGAGP"
     gene            10208..10603
                     /locus_tag="P5313_08485"
     CDS             10208..10603
                     /locus_tag="P5313_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80438.1"
                     /translation="MRRAGGPRMPARRAAVLLASAVPVLILAACARDWLPSGNARVLG
                     WHDEEVGGIPSCVAYLEVSNVGSVRIERSSLSLRVFTASRSYFRTLVLQTPLPPGGLV
                     YAELSVAYADPGESAEADGGVELVDCFFE"
     gene            10785..12029
                     /locus_tag="P5313_08490"
     CDS             10785..12029
                     /locus_tag="P5313_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRY80439.1"
                     /translation="MDSSLLRAYANVLLTAVNLQKGQCLLIRAQPVHLPFAGILAEEA
                     YKRGGRYVRFDNNEADNPALYKARIEHSEAEYLEYAPRSRYEPYGTMIDEDWAVLSIH
                     APEDPGFLSGLDPKRNARCVRATAEAMKPYRRRIVLDNEVAWLVAFLPTEKMAARILG
                     VPAGPEAVDELWKVLIPILRLDRPDPSKAWEEHCRNLEARAERLNSLSLDSVRFRGPG
                     TDLAVGLLPQSVWRGGPERTAKGRLFFPNIPTEEIFTSPDFRRAEGRAAFTRPVLVPS
                     IGKVVEDGWVEFRDGRAVDWNARSGKDVLDTLMSMDPGARSLGEVALVDGSSPVFTSG
                     RVFHNILFDENAASHVALGSGYPACVRGAEGLSDGELAELGVNVSMVHTDFMIGSPDM
                     DVTGTTRDGREVRIMTRGRFEV"
     gene            complement(12089..13693)
                     /locus_tag="P5313_08495"
     CDS             complement(12089..13693)
                     /locus_tag="P5313_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY80440.1"
                     /translation="MPRTIVLNHVSFRYESLPRPVFSGLTLSFPPGWTALVGPNGCGK
                     STLLSLVTGRLTPESGSLVPARDASVCGQGLDMPEAFFSDPELMGDPDTRALLSRLGI
                     REDFPGRWGTLSGGEKRKCTVAAALAGKPEVLLLDEPANHVDADTLDLLAAELARFNG
                     IGILVSHNRSFLDRVCRRTVLLTPGEAGSGARTFACPPGAALQESLREDDFLRGERAA
                     LEARLRRLERRRILATREAAAGAARLSKRDLDPRDASGRAEIDRARLTGKDGRAGRSA
                     GKLAGQAGRVRAELESRPAPGLRKSGAALPGRRERRPVLLDRAPGILDLAGGTVRLEH
                     PRLEIRNDGRTAILGPNGSGKTSLLRWIRGSLDLPEDRVWYLEQELSERSRREALSAL
                     RTLGAEDRGRVLSAVYRLGSEPESLLSTDLPSPGETRKLLFALALLRGVSAVLLDEPT
                     NHMDPGSAACFSEALADFEGAVVLVTHDEAFTGRVAEGRWRLERDGIRTVLSVTGGED
                     PTQQGVEKGYRAFFNTSLMSQLRREP"
     gene            14205..15515
                     /locus_tag="P5313_08500"
     CDS             14205..15515
                     /locus_tag="P5313_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRY80441.1"
                     /translation="MASGEKKKGWLGWYFNTPLVWRILTALVTGAIVGLLVGPSISVI
                     APLGSLMIRLLKMIILPLIFFAIVMGIGSMPASKIGKVAGKILIYYFGTTLFAAIFGL
                     ILANIFKPGLGLSLTATAVKAAEGATVTSPAIGDVFLNMVPDNVAAAFAKGAYLQVLV
                     FALFFGLAVSFLRDHKDDRISEAVLTVYRFCEGGAEIMFRITHAVLQFAPIGIFAIIS
                     GIFAVEGTKLISSAASIIALCYTGYAFQLFVVYGIALTLSKLNYFQFLSRAKDATLMA
                     FSTCSSSATLPLTIKTSEEKLGVPRSIGGFTLPLGSQINLDGEAYYQIIAVFTAAFAS
                     GIHLTFAQQMVVVFVVTVGTMGTAGIAGSGPVVLLAVMGMVGLNVEAGSMVAAVFALV
                     LGIDVILDMGRTAVNVTGDMVGTCIVAKSEGLLKKWGPKGTFVA"
     gene            15651..>16129
                     /locus_tag="P5313_08505"
     CDS             15651..>16129
                     /locus_tag="P5313_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HRY80442.1"
                     /translation="MNRLPASIFNDVIGPVMRGPSSSHVAGAARIGSLVRQSLRGNVQ
                     EVEVEFDVNGSLAESYHGHGSDIGFASGILGMELTDESVPLACRIAEERGIDVRYKVL
                     DYGAVHPGNYRITAQAPDGTFVYPGTCRGLDPGRRRSLIEAGRADGFRLDVAKAGRRT
                     "
BASE COUNT         2482 a         5422 c         5506 g         2719 t
ORIGIN      
        1 cttttccggg gacctctacg acttcgagcc gaccctgccc tccgcctgcc ccggagacct
       61 ggggctctcg gtccccgcca aggtactgcg gtccgtctgg aacgccctga agctcctgga
      121 caccatcgtg cccggggtcc tgcaccccag caccatcctc tactacccgg agatcaagct
      181 gtacgccaac cgacctgtct tccgggacga ctcgttccag gcggccccgg ggatccgact
      241 ggtcggggac ggggcgggga ccagccgggg catcaccggg gcctgggcct cggggatccg
      301 ggcggcggac gggatcctcg caggttgttg aagtactcga agcatttcaa caccctgcta
      361 ggggccgcat aggcgcccct cccctcggcc ggcgtatctg ccccggggct ggcatgccga
      421 ggcggcatcc gctaagcttt ccgcatggat gaatcggccg atccccgtga ggaccccccg
      481 gacccggccg ccgccgtttc ggcgccgcgg cttccgaacc ggggggcctg tctcagcgca
      541 tccgccctgg ccggctcttc ggccctggcc gtctacgcct tccttgcgca ggaaggtgcg
      601 gcccgtacgg ccttcggcct gctccactcc ctgcagaccc tgttctggct gggcggcctg
      661 gcggtctttc tactccgacc ctcgtacaac cgaaggacgc cctacgcgcg gttttccggc
      721 atcgccgggg gagtcctcct cgggatctcc ctggcctacg gcgcggccgc agcctggacc
      781 ggcgcgggat tccccatctg ggcggccttc gcggtcgcgg gcctgtccct cgcccggctt
      841 ccccggggat accgcggcgc ctatgccctc ctggcgggca cggctctttc gctcctctcc
      901 ttttacctac gtggagtcca ggccctgcgc gcggatctcg tatacctcct ggcacccctg
      961 gctctgggtc atgcggcggg atccttcgtg gatcggatcc gcacggaggc ggacctgtcc
     1021 ctcaaggatc tcaagtccca gaacgaggag ctggaggaac tcgcctaccg ggatcccctg
     1081 acggggctct acaatcgacg gttcgggctt gaaagcctcg ggaacgccat ctccttcgcg
     1141 cagcggtata agcaggaact ccacatcttc ttgctggatc tcgaccactt caagagagtg
     1201 aacgacgaac tgggacatcc cgcgggagac cgggtccttg tgcagctggc ccaattgctc
     1261 cgcaaggcgc tccgggagag cgatatcgtc gcccggatcg ggggggagga attcctggtg
     1321 atcctgcccg tttccagggc ggaactggtc catggagcgg cgaaccgcat ccgggacatc
     1381 acggcccgga cgaccttcga gccggtgccc tggcaggtga ccgtgagcat cggggtgacc
     1441 ggccttcggg agggcgacac cccggaatcg atgctttccc gggcggaccg gttcctctac
     1501 gattccaagc ggggcggccg gaaccgggtc accggctcct aggagcctgt cggacttgtg
     1561 catttttagc gccgataggc gctaaaaatc ccgattatgc ggctcctagg tgtcctgttc
     1621 tacaggatgc gggtcaggat ggccacgtag gccacggccg cgaccaggaa tagcccgagg
     1681 ggcgccgcga agcggcggat ggtccggggc agggtggcct tgaagtgctc cgcgctcacg
     1741 accatgcaga cgtgcagggg ggacaccatc atcccggcga acccgaaggc cgcgaccagg
     1801 gccacgatgg gatccagggc cagggagccg aaggagcccg cccggaagag tcccagcacg
     1861 atgggcaggg aaagtcccac gtagccgaac cccaccccgg tgaccagccc ggagatcagg
     1921 ggcaggagag ccgccacagc caggggcggg atgcccgcgg ccgcgagttc ggaggcggcg
     1981 gattgggccg ctcccccctc cgacaggagg gcggagaaga cgcggatgcc cgccacgacc
     2041 cccgccagct tcagggttcc cgggttgaag gtatccttcc agacggcccg gccgcggttc
     2101 cggacggcga cgtaggccat gcccgcgccg atccccgtga ggatggggaa gtagcggccg
     2161 gccagggcgg agacgggacc gggtagaccc agggacggcc ccagggcccg ccaggccaga
     2221 tccagggccg catagacccc caggatcagg atgaggggag cgaaccccgc gatcacctcg
     2281 cccgctccgg tcctcggcgc ggcagcggat cccggaccgg tccgctccgg ggagggcgtc
     2341 cccgcggcgc cgcgtccggg gagtttcagc aggaagagcc agcccaggaa gaccatggtc
     2401 gggaacatgt agaagttgat caggaccagc cgggagacct cgaagccggt cagggtggcg
     2461 gtcaggatga aggacgggaa gaggggccac cagacctcca gggagtggcg gaaccagtag
     2521 ttggccgcgg ccagccggtc cgcccccaac cgcccttcgg gatccagggc ctccaccagg
     2581 ggggcggaaa aggcggctcc cccgggcatg ggcagggtcc cgatcaggac cggggtcagg
     2641 atgatggagg cgttccggga ccgggccacc gccttcacgg accgggcgaa gtcctccatg
     2701 gccccggcct tccgcatggc cccggacagg accatgatca gggtgaccag gaggaggagc
     2761 aggaggttct cggccgccag gaactcttgc agggagcgca ggaccacgcg cccgaagggc
     2821 agcccctgcc agagggcgaa ggcccagccc ccggccaggg cggccagccc cagatggagc
     2881 ttccgggaag cggcggccac gacgattccg aacaccacga ggatcttaac gaggacgggg
     2941 atggaagcca tggtcggact cctgtcaggg tttcgagcag gccgggcagg tgccgtggaa
     3001 gtcgaggcgg tggccccgga cccggaaccc ttcccgggcc gcggcttcgt cgagttcggg
     3061 gcggtagggc aggggcatgt ccaggacggc tccgcaggcg tcgcagacga agtggtagtg
     3121 tggggacacg tccccgtcga accggtcctg ggcgtttccg ttgcggatgt gcaggaccag
     3181 gcccatctcc tccaggacgt ccaggttgcg gtacacggtg cccagggaga gccggggtat
     3241 gcgccgccgg gccgcggtgt ggagcatggc ggccgtggga tgttccttcg tggagcggag
     3301 gaccgcgagg atctcttccc gctgacggga tcgttttctc gagcgctcta tgccttccat
     3361 cctgccgcct taggagttga gccaatttca aaagtcggtc acttttgaaa ttggcttcgc
     3421 gattgcttgc ggttgcaacg caaccgcaag caatcgctta tttcaaggta gctttttcaa
     3481 aactcagaga gttttgaaaa agccccagtt gttagggtaa gagggtacaa tccatcccgg
     3541 tttccgtcaa gcaatccgtg ccgccggctt gccccggagc gtcaacttgc gcccggaagc
     3601 tccaatcggt acaatcgccc atcatggaaa cccgtgccgt ccttccggcc gcctgtttgg
     3661 cggttctcct gaccgcggcc tgcggcgtcc gcgggcccgt cggccaggcc cccgcggagc
     3721 ccccccgggc ggtccggggc cagctggatc tctccgcctc gaaggagggc gtcgttgccc
     3781 tggacggccc gtgggccttc tggtggggtc gcttcctgga tcccccggac ctggccgacg
     3841 gttcggaggc ggcgccggac ggtttcgggg aggtgcccgg ggcctggaac ggttacggta
     3901 ttccggaggc ggtccgccag gggcacggca cctaccgcct gacggtccgg ggcctggatc
     3961 cggccgtccg ctacgccctc cggctctcgt ccttcaatac agcggcccgg gtattcgcca
     4021 acggccgggt cgtccacctc caggggatgc cgggcggctc ccgggacctg gaggagccgg
     4081 actggaccgc ggtcgtggtg cccgcggatc cggattcccg gggcgtcctg gacatcgtcc
     4141 ttcacgtctc caactacgcg gaccgcaccg ggggcttcgt gaaccgcgcc ttcctgggcc
     4201 cctacgaagt cgtggacacc ctgcgggacc gggaacggat gaccgaggtc ttcctgttcg
     4261 gcggcatcct cctcatgggg ttctactacc tggccctctt cttcttccgc cgcaaggatc
     4321 gggcctccct ctggttcggg ttcctgtgca tcgtcctggc gttccgcatc ctctgctacg
     4381 acgagtacct tatccgggaa ctcctgccgg gtctgccctg ggtctggctg ttccgggcgg
     4441 ggtacctgac cttctccctg gcggtcttcc ttatagcggc cttcctgcgg agcctgttcc
     4501 cggcctcctg gtggaagccc ctgtcctggg tcatctacac aggggcggcg gcgtactccc
     4561 tggtcgtggc cgtctttcct cccctggtct ccgggaacct cctcacggcc ttccaggtct
     4621 tcgccctggc ctccggggcg gcccttttcg cggtgctgct ccgggccctc ctgtcccggg
     4681 aacccggggc ggccctcctg gccggagggt tcggctgcct cttcgtatcg gtggtccacg
     4741 acgtcctagc ctcccagggg atccttccgg gccccttcgt ggtgcaggtg ggcctcctgt
     4801 tcttcctgtt cgccatgtcc ctggtggtga cccgcaagtt cgccgtatcc ttcgaaacct
     4861 ccgagcggct ggcggagagc ctccggcgta tgaacctctc cctggagcgt ttcgtgcccc
     4921 gggagttcct ccggtacctg aacaagagct ccctagacga gatctccctg ggggaccatt
     4981 ccgcccagcg catgacggtc ctcttctcgg atatccgctc cttttcccgg atctccgagc
     5041 ggctctcccc ggaggagacc ttccggttca tcaactccta cctggaacgg gtcgtcccct
     5101 ccatccggag ccacgggggc ttcgtggaca agtacctggg ggacggggtc atggccctct
     5161 ttccccagga cgcggacgcc gccgtccgct gcgccgtgga catgcagcgc cgcatctcct
     5221 ccttcaacca ggagcaggcc gggaagtggc tggaacccgt ctccgcgggg gtcggcatcc
     5281 actcggggct tctcatgctg gggaccatcg gagagaacga gcgcatggac gggacggtca
     5341 tctccgacgc ggtcaacctg gccagccgcc tggaggggct caccaaggag tacgagatcg
     5401 gcatagccgt cagcgaggag atcgtggaag ccctgaccga ccgctccgcc tggaagactc
     5461 ggtacctggg caagatcggg gtcaagggca agcgggagca cgtctcggtc ttcgaggtct
     5521 acgacgggga tcccgaggac ctgatcgtcc gcaaggactc catccgcgga accttcgagg
     5581 aggccatcgc cgccttctac cgccaggatt tcccgtccgc catggcgggg ttccggtccg
     5641 tcctggagat cctgccccgg gacgaggcca gccactacta catccggatg atccgcaaga
     5701 tgaacctctc ctaggagttg tcgggcttgg ttcgtgcaat gcataaatat gcgcatttga
     5761 gcgccggttt ccctatcctc ggaggtatct cgtttcgggc ggacgcggta ttgcaaattt
     5821 atccgcaatg tcctatggtt cgaaccgacg cggcgggacc gcccggcgcg cataggaggt
     5881 gcgaggcatg aagaaaaccg cgatcgccct ggctctttcc atggcgatcc tggccctggc
     5941 cggatgcggc gggggcaagg cggagaagaa ggtcctgtac atctacaact ggacctacta
     6001 catccccgac gacgtagtgg aggacttcga gaaggaattc aactgtgaag tcgtgtacga
     6061 tgtgttctcc tccaacgagg acatgttcgc gaagatcaag gccggcggct cgggatacga
     6121 cgtagtcttc ccgggagccg acttcgtctc catcatgata aaggagggga tgctcgagcc
     6181 cctcgatccc aagaagatcc cgaacctgaa gaatctggat cccgcggtga cctcccggat
     6241 gagcttcgac ccgggcaaca agtactcggt cccgtacttc atgggagcca cgggcatcgt
     6301 ggtgaacaag aagatcgccg ggaacttcga gcgcagctgg aacatctacg agcgcaggga
     6361 cctcaagaac aagatgaccc tcctggacga cccccgggag accatcgggg cggccctcaa
     6421 gtacctggga tactcggtca actccgtcaa tcccgacgag ctggccaagg cggaggccca
     6481 gatcaagaag tggctggaaa acgtcctgcg cttcgactcc gagtccttcg gcaagggcta
     6541 cgccaccggc gagtacgcgg ttgtccacgg ctgggccgag aacgtcttcc tggagctgga
     6601 cgacgccctg aaggcggacg cggagttctt catcgccaag gagggcgggg tcgcctacat
     6661 ggactgcatg gccatcctca agggcgccaa gaaccgcgac ctggcccacg ccttcatcaa
     6721 ctacatccag cgcgacgaca tctacgcccg catcgccagc tatctggccc tgcccagcgt
     6781 gaacgttccc gcccgggcca agctgaccga gaagcccaac tacaccgagg cggacctggc
     6841 gcgctgcgag ctcaagctgg acatcgggga ttccatcacc ctgtactccg acctctggaa
     6901 gcgcctgcgt ctgggcgatt gatccgacct cggccggttc cccggccggg gcatccgagc
     6961 gcacgatacg gcgggacgag agtcctgccg tttttttgtc cccgacgcaa gcgcccgggc
     7021 gatccgcggg cggatgcacc tccgccggga cgcgttcccg gccgccgggc atgagcccgg
     7081 cgcatccgga acaaggaggc gcaccgtgaa aaacctgtgg attgtcctgg cagcggccgc
     7141 ggcgttcgcg gcccccgcct tcccgcagga accggatccc tttgccgggc tcgactcgat
     7201 cgccccggcc ctggaccgcc cgggggacta ccgagggggg gacgtgttca tccgggccag
     7261 cgcccgcagg gaccggctgg tggtggtgga gatccccatc gacggccggg gcaatgcggg
     7321 ccggtacgag gtggaagccc acaatcgggt gaccggggga gtgagcgcgg atctgatcac
     7381 cagggatggc aaggtgcccg gctacgcggc ggtgccggaa gggttcccgg agggcaccca
     7441 ccgggtcacc tccgtgagcg cccgcacgga caagttcggg ccgtattcaa tcggcaccga
     7501 cgcggaaggg ctggtggacg cgtacaaggt gaccaagcag gacggaaaga tcgtctcctc
     7561 cgagtacgcg gggaaggtcg cggactacgg gtacagcatc cactccaacc gggagaagtc
     7621 cttcgagacc tcccggtcct ggggctgcat catcgtccgg gaggcggact gcggcaggat
     7681 tgcccgctcg atccgggcgg accgggcggc gggcggggac cagtacgtag tggcgggccg
     7741 gtgaaggcgt caaagaccgg tccgtgtttt taggaaatca ccgcggagga cacggagagc
     7801 acggagccca ggctcccgct ctccgtgcgc tcccctcatt cgaagaggga aacccgaaat
     7861 aggaggaacc catggcgcac aagggatttc gcatgaccgc ggcccgcatc ggccgtatcg
     7921 cccttctgac cgcaggtctg gttgccggcc tggcggccgg ccttgcggcc tgcggcccgg
     7981 gagcttcgga gcgggcgccc gcggaggcga aggccccgtc cggtacgtcc ggcgccccgg
     8041 ccgccgctcc ctcgaccggg gcgggtccgg cgtccgggcc cgaggccgcg gtcttcgagt
     8101 atcggtacac ctcggttcgg atcgaggatc ccggcgccgt gcggtaccgg gccgagttcc
     8161 gcttccatcc cgacgggacc atccgccggg cggaactgcg ggaaatccgg tccgggacgg
     8221 agaccctccg ggcctccgcg gtgtccgagc ccttgcaggg agggtacaag gtcgcctacc
     8281 gatccttcgg cagggacccg aggggctacg actataccct gggatggtcc gaaacggggc
     8341 ttacggtcac cgcgggcggg gaggcgacgg ggttccgctt cgacccggcc ggccgggtct
     8401 tcacggtccc ctgggacaag ggcgcggaaa cctactcccc ggggccggac ggcagcgtcc
     8461 tgggggagct cgtcctggcg ggcgagcggc agtggggcgg acgattgttc aagagcgggg
     8521 gaagcctccg gttcgaagac cggtatcccg acgggagcgt ggaccgggag tgcgtcctca
     8581 aagccgcggg cccggacgcc tggtccgtat cctgcacggg ttccgcggac tccttctacg
     8641 agggcagcct caccggggct tccctcttcc cccggggagg cgtatacccg gcctcggcct
     8701 acaacctgat catcgtgacg gacctctgga tgaccgagga acggtttccc ttcttctgcc
     8761 tctccctgct ggagtgagga cgtggaagcc ctgttcctgg cctggatgat ccgggccgcc
     8821 gtcggccgag aagccctgcg cttcgaggcg gtccacgagc aggaggggga tctctcctgc
     8881 ggcctggccg cggccgcctc cctggcggac ctgtactggg ggcttccctg ccgggaggcg
     8941 gacctccggt ccgccctggc gcccgaggac ggccgggtgg agcgggcgac ggatctggga
     9001 gagctcgccg ccctgctctc ggcccggggt ttcgcggtcc gggccgtccg gctggatgcg
     9061 gtctccgccg cggaggccct tcgacggggc tacgtccccc tcctcgtgca cctccgggac
     9121 gacggtgggc acttcgccct ccttttggcc ttggaacggg atgtcgttgt cctggcggat
     9181 ccggcccggg gcttgttcac cctggacacc ggggagtttg ccgcctcctt ttcgggcggg
     9241 gccctggccg tcgtcttccc gggcgtccgg gtcgatcggg atcggttgga cgcccggacg
     9301 gccggggccc tgggagcccg ccgtttcctt gccggcctgg ccgtgggggc cttccgttga
     9361 agactcccgc cccggcggcc gccctggccg cggcggtggc tgccctgaca gtctccgtcc
     9421 ctcccgcggc ctgctcggaa gctccccgcg cgcgaaccgg ttcggaggag cccccggccc
     9481 tgtccttcct ggcggaagtg cggccgatcc cgcgggatct cccggtgggc cgggtactgg
     9541 acgcggactg ggagttcggc ctggacctcc gccctccggg aatcctccga ttctcgatcc
     9601 gactgcccct gtccgtgcgg atcggccagg agggcccctg ggcgggccgc tcggacgcgg
     9661 cctgggggga cccggccgtg gaggcggggg cctttttccg gaagggcgcg ggcttccggt
     9721 tcctgggggc cgggtacgcg gcacccctgg gccggtggat gccggatccg gcacgacccc
     9781 tccgccccgc cccgggttcc ggggcccacc gcgcgaccct gtccgcgggc ctgggccgga
     9841 tccgggatcc cgccgccctg agcgcggggg tttcctggac tgtctcgatt ccccggccgg
     9901 atattcccgg agccgcctgg cgccccgcgg atctgtccct gtccctgtcc ctcgtcgagg
     9961 tcttgaacga tcgggcgggg atcctgctgg gcctctctcc ccggctccgg gctcccgccc
    10021 gggggccggg ggcggacccg gaatcgggat tcgactgggc cctggaggcc cgggtagagg
    10081 tccacctgcg gaccgggggg gccttcttcc gtaccggagt aagccgatcc ctgaccgaac
    10141 cgggcgccgt ctgggttccg gtcgcgggag tggaatacga ggtgcggata cggacggggg
    10201 ccggaccgtg aggcgggcgg gcggcccccg gatgcccgcc cggcgggcgg cggtccttct
    10261 ggcctcggcg gtcccggtac tgatcctcgc ggcctgcgcg cgggattggc tcccctccgg
    10321 gaacgcccgg gtcctcggct ggcacgacga ggaggtcggg gggatccctt cctgcgtggc
    10381 gtacctggag gtctccaacg tggggtccgt ccgcatcgag cggagttccc tctccctccg
    10441 ggtcttcacc gcgtcccggt cctacttccg gaccctggtt ctgcagactc ccctgccgcc
    10501 gggcggtctc gtgtacgcgg agctctcggt ggcctacgcg gaccccgggg agagcgccga
    10561 ggcggacggg ggcgtggagc ttgtcgactg cttcttcgag tagcgccttc ccggcgggtt
    10621 tagcaggttg ttgaaatact cgaggcattt caacaacctg ttaaagggcg gcccgtggag
    10681 aaggggtgtc caataaggga ttgacgtatt ggacaccccg gttcggcctt gacgggtccc
    10741 cgcggcggac ctaggatggg ctatcctgcc cgccgggagc cgccatggac tcgtccctcc
    10801 tccgcgccta cgcgaacgtc ctcctgaccg ccgtgaacct gcagaagggc cagtgcctcc
    10861 tgatccgcgc ccagccggtc cacctgccct tcgccggaat ccttgcggag gaagcctata
    10921 aacggggcgg ccggtatgtg cggttcgaca acaacgaggc ggacaatccc gccctctaca
    10981 aggcccggat cgagcacagc gaggcggagt acctggagta cgccccccgg tcccggtacg
    11041 agccctacgg taccatgatc gacgaggact gggccgtcct gtccatccat gcccccgagg
    11101 accccggctt cctgtccggc ctggatccca aacggaacgc ccgctgcgtc cgggccaccg
    11161 ccgaggccat gaagccctac cgccggcgca tcgtcctgga caacgaggtg gcctggctgg
    11221 tggcgttcct tccgacggag aagatggccg cccggatcct gggtgtcccg gcgggaccgg
    11281 aggcggtcga cgagctctgg aaggtcctga taccgatcct ccggctggac cggccggatc
    11341 cctcgaaggc gtgggaggag cactgccgga acctggaggc ccgggcggaa cggctcaact
    11401 ccctttccct tgactccgtg cggttccggg ggccgggtac ggatctcgcg gtcggccttc
    11461 ttccgcaatc cgtctggagg ggagggccgg agaggaccgc gaaggggcgc ctcttcttcc
    11521 cgaacatccc caccgaggag atcttcacgt ccccggactt ccggcgggcc gaaggccggg
    11581 ccgccttcac ccggcccgtc ctggtaccct cgatcgggaa ggtcgtggag gacggatggg
    11641 tggagttccg ggacggacgg gcggtcgatt ggaacgcccg gtccgggaag gacgtcctgg
    11701 acaccctgat gtccatggat cccggggcca gatccctggg ggaggtcgcc ctggtggacg
    11761 gatcctctcc cgtcttcacc tccggcaggg ttttccacaa tatcctgttc gacgagaacg
    11821 ccgcgtccca cgtcgccctg ggatcgggtt acccggcctg cgtccgtggc gccgaggggc
    11881 tgtccgacgg ggagctcgcg gagctgggcg tcaacgtctc catggtccat acggacttca
    11941 tgatcggaag ccccgacatg gacgtgacgg ggacgacccg ggacggacgg gaggtccgga
    12001 tcatgacgcg gggacggttc gaggtctagc aggttgttca aatacttgag gcatttcaac
    12061 aacctgctat gcaattgcgc agagaacctt acggttctct gcgcaattgc gacattaagg
    12121 aagtgttgaa aaaggctcta tagccttttt caacaccctg ctgggtcggg tcctcccctc
    12181 ccgtcacgga aaggacggtc cggatcccgt cccgttccag gcgccaccgc ccctcggcga
    12241 cgcggcccgt gaaggcctcg tcgtgggtga ccaggactac cgctccttcg aagtccgcca
    12301 gggcctccga gaaacacgcg gcggatcccg ggtccatgtg gttggtgggc tcgtcgagga
    12361 ggaccgccga cactccccgc agcagggcca gcgcgaagag gagcttgcgg gtttccccgg
    12421 gactgggcag gtccgtggac aggagggact ccggctcgga tcccagccga tacaccgcgg
    12481 acagtacgcg gcctcggtcc tccgccccga gcgtccgcag ggcggacagg gcctccctcc
    12541 ggctccgctc ggaaagttcc tgctcgaggt accagacccg atcctccgga agatccaggg
    12601 agccgcggat ccaacggagc agggacgtct tcccgctgcc gttgggaccg aggatcgccg
    12661 tgcggccgtc gttccggatc tccaggcggg gatgctccag gcggacggtc cctcccgcga
    12721 gatccaggat tcccggcgcc cggtccagga gcacggggcg ccgttccctc ctgcccggca
    12781 gcgcggcccc cgacttgcgg agacccgggg ccggccggga ttcgagctcc gcgcggaccc
    12841 gaccggcctg tccggccagt ttcccggcgg accgtccggc gcgcccgtcc ttccccgtca
    12901 gacgggcgcg gtcgatctcc gcgcggcccg aggcgtcgcg gggatccagg tcccgcttgg
    12961 agagccgggc ggctccggcc gccgcctccc gggtcgcgag gatcctccgc cgctccaggc
    13021 gcctcaggcg cgcctccagg gcggctcgtt ccccccgaag gaagtcgtcc tcccgcaggc
    13081 tttcctgcag cgcggcgccc ggcgggcacg cgaaggtacg ggctccggat cccgcctccc
    13141 cgggtgtcag gaggaccgtg cggcggcaga ccctgtccag gaaggaccgg ttgtgggaga
    13201 ccaggatccc gattccgttg aaccgcgcga gctccgcggc cagcaggtcg agggtgtccg
    13261 cgtcgacgtg attcgcgggc tcgtccagga gcaggacctc gggttttccg gcgagggccg
    13321 cggctaccgt gcatttgcgt ttctctccgc cgctgagcgt tccccaccgc ccggggaagt
    13381 cctcccggat cccgagccgg gagagcaggg ctcgggtgtc cggatccccc atcagttcgg
    13441 ggtccgagaa gaaggcttcg ggcatgtcca gtccctggcc gcagacagac gcgtcccggg
    13501 ccggcaccag gctcccggac tcgggcgtga ggcgtcctgt gaccagggac aggagggtgg
    13561 acttcccgca gccgttcggc ccgaccaggg cggtccagcc cggtgggaag gagagggtca
    13621 gcccggaaaa gacgggccgg gggagggatt cataccggaa ggagacatgg ttcaggacta
    13681 tcgttcgggg catcgaggga gatccagatt tcggattcag ccatgacaag cgctgcaggc
    13741 ccgacgtatc cggaatctcc gggcggacgg gcagggggaa acggaaaccg tcacgggagg
    13801 cccggccggt acgacgtacc ttttaccggt tctccgctgt tctgttccgc gttcccttac
    13861 aggctcatgt cccttcgatc cccccggtta aaggttcttt atttttccga acagtaccgg
    13921 aagtacggat ccgttgtcaa gaacggcccg ccgcggcgtc cgaccgcttc ccgcggggtc
    13981 ggcccaaaaa agtattttgt tgacagagta aaatacatcg cctacaatgt aaaataacgc
    14041 ggaagtagag tccgatggcc gcgtggaaag ccgtaccgcg acgagcccct gccgaccgcc
    14101 cgtcgaggac gattcccgcc cgaaggctca cgcgccttgg acttgacgac cggtcccggt
    14161 atagggaccg gaatgatcca tttcctcatt tcgggaggtt ccagatggca agcggggaga
    14221 aaaagaaggg ctggctcggg tggtatttca acacgcccct cgtgtggaga atcctgacgg
    14281 cccttgtcac gggcgcgatc gtgggcctcc tggtgggtcc ctcgatctcg gtcatagcgc
    14341 ccttgggaag cttgatgatc cggctcctga agatgatcat cctgccgttg atcttcttcg
    14401 cgatcgtgat gggcatcgga agcatgcccg cctccaagat cgggaaggtg gccggcaaga
    14461 tcctgatcta ctatttcgga accaccctgt tcgcggcgat cttcggcctg atcctggcga
    14521 acatcttcaa acccggcctg ggtctttcgt tgaccgcgac cgccgtgaag gccgccgagg
    14581 gggcgaccgt gaccagcccg gccatcgggg acgtcttcct gaacatggtg ccggacaacg
    14641 tggccgccgc gttcgccaag ggagcttacc tccaggtcct ggtgttcgcc ctcttcttcg
    14701 gcctcgccgt ttccttcctg cgggatcaca aggatgaccg gatcagcgaa gcggtcctga
    14761 cggtgtacag gttctgcgag ggcggggcgg aaatcatgtt ccgcatcacc cacgcggtcc
    14821 tgcagttcgc gcccatcggt atcttcgcga tcatctcggg gatcttcgcc gtggagggga
    14881 cgaagctcat cagctccgcg gcctccatca tagcactctg ctacacgggg tacgcgttcc
    14941 agctcttcgt cgtctacggg atcgccctga ccctgagcaa gctgaactac ttccagttcc
    15001 tgtcccgggc caaggatgcc accctgatgg cgttctcgac ctgctccagc agcgcgaccc
    15061 tgcccctgac gatcaagacc tccgaggaga agctcggcgt tccccgcagc atcggagggt
    15121 tcaccctgcc gctcgggtcg cagatcaacc tggacgggga agcctactac cagatcatcg
    15181 cggtgttcac cgcggccttc gcgtccggta tccacctgac cttcgcccag cagatggtcg
    15241 tcgtgttcgt cgtgaccgtg ggcaccatgg ggaccgccgg catcgccggc tccggaccgg
    15301 tggtgctgct ggcggtgatg ggcatggtgg ggttgaacgt ggaagccggc tcgatggtgg
    15361 cggccgtgtt cgccctggtg ctcggcatcg acgtgatcct cgacatgggc cggaccgccg
    15421 tgaacgtcac gggcgacatg gtcggcacct gcatcgtggc caagagcgag gggctgttga
    15481 agaaatgggg ccccaagggg acgtttgtcg cctgatcccg gtccgataga atgatgtgaa
    15541 cggagggtca ttgcagaggc ggggacctgt accgtctttg caatgaccgt ctctcgaggc
    15601 tagcattgtc cggggcattc gcacgggcgg atcgagcggg ggataggtcc atgaacagac
    15661 ttccggccag cattttcaac gacgtcatcg gtccggtcat gcggggaccg tccagttccc
    15721 acgtcgcggg agccgcccgg atcggttccc tggtccggca gtccctgagg ggaaacgtcc
    15781 aagaagtcga ggtggaattc gacgtcaacg gctccctcgc ggagtcgtac cacggccacg
    15841 gttcggacat cggcttcgcc agcggtatcc tggggatgga actgacggac gagtcggtgc
    15901 ccctggcctg caggatcgcg gaagagcggg ggatcgacgt ccggtacaag gtcctggact
    15961 acggagcggt ccatcccggg aattaccgga tcaccgcgca agccccggac gggaccttcg
    16021 tctatcccgg cacctgccgc gggctcgatc ccgggcgccg ccgttccctg atcgaggccg
    16081 gccgggccga cggcttccgc ctcgacgtcg ccaaggccgg gcgcaggac
//
LOCUS       DAOTEI010000072        15822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2521_length_15822_cov_4.553625, whole genome shotgun sequence.
ACCESSION   DAOTEI010000072 DAOTEI010000000
VERSION     DAOTEI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15822
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2521_length_15822_cov_4.553625"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="P5313_08510"
     CDS             <1..585
                     /locus_tag="P5313_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRY80443.1"
                     /translation="DPGADLVAEAHRLGIRVVPLPGPSSILQALMASGFSGQRFEFLG
                     YLPPRGEDRRQALKRVEREASRDGSTRIFIETPYRNQALLDDALAVLEPGTRFCVAIS
                     LGGASELVRSMEIHRWRAAGIHLEKEPAVFLLAPKAVPGALTGRPSGEAAVKREGPRS
                     GSAARQAARPSADRHGSPRTGQTARRRTRKNPGA"
     gene            619..1428
                     /locus_tag="P5313_08515"
     CDS             619..1428
                     /locus_tag="P5313_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80444.1"
                     /translation="MVKTDKVNVSETYAESVVSLSPFALKKLGIISNQTLLKVDTYNL
                     VCIPYRLSMKGAVLLGSFSRDEIVFFQRFKGSLAGLNLVLQPGDSPAPRKVFCRCSLA
                     QFAPMKGRESVGLISVEFRPCPPDLENLIGGYVMLMDRLRADYRDLSGKTVPVNPETA
                     KILGFNNYATLNSGSIVTKLALFTLGADRLVFLAPMQTPDLQPGQTATLRLFFQKYQF
                     NVAGRITESIRLPTGVQKGTVELEFSPELVDLLEMYYLEAKLGARIPKSAV"
     gene            complement(1455..2471)
                     /gene="queA"
                     /locus_tag="P5313_08520"
     CDS             complement(1455..2471)
                     /gene="queA"
                     /locus_tag="P5313_08520"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRY80445.1"
                     /translation="MRTKDFSFDLPEDLIAQRPSGERGASRLFVLDRGTGTFTHSAVS
                     DLARHLPPGSLLVFNDSKVRKARIYAESGTGGRVEFLLLRRRGDREWLAMTTKARKQV
                     PGKRYAFPGGIEGRIVGTEEDLRILELDRPIDDEYLDRWGHMPLPPYIRRQDDGFDAE
                     RYQTVYSRTIGSVAAPTAGLHFTDGLLASLRTAGMETAQVTLHVGLGTFLPVRAERIE
                     DHVMHEEEYSVPEATAEAVNRARREGRPVVAVGTTSVRTLESAWLDGSLRAGSSSTRI
                     FLYPGRSFQVVDALFTNFHTPESTLLMLVSAFAGRELILKAYEEAVRLRYRFFSYGDA
                     MLIL"
     gene            complement(2446..3453)
                     /gene="ruvB"
                     /locus_tag="P5313_08525"
     CDS             complement(2446..3453)
                     /gene="ruvB"
                     /locus_tag="P5313_08525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRY80446.1"
                     /translation="MLDPGLRGAEDDKEGVLRPQRLSEFLGQERLKENLSVFIRAARE
                     RGEALDHVFLLGPPGLGKTTLAGIVSRELGVDFKVTSAPALDKPKDLAGILTTVTERT
                     VFFIDEIHRLKPAIEEMLYIAMEDFELDWIIGQGPSARTVRIPLPPFTLVGATTKAGM
                     VSSPLVSRFGISLRFGFYEPREIILILNRSAAILGMELDPGAADLLAASSRGTPRVAN
                     RLLRRMRDFAQVFGEGRVTEGVVKEGLARLEVDRYGLERHDRDILRSVIEKYGGGPVG
                     AETLAISVGESVETLEDYCEPYLIQAGFLQRTPRGRVAAPLAYEHLGYRNGHADERLL
                     F"
     gene            complement(3483..4067)
                     /gene="ruvA"
                     /locus_tag="P5313_08530"
     CDS             complement(3483..4067)
                     /gene="ruvA"
                     /locus_tag="P5313_08530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRY80447.1"
                     /translation="MFNSLLGTITEKRFDILRVDNNGLEWEFSVPARSIDEFGTLGGR
                     TKVYIHLLHREDQMRLFGFPSERERALFLELLKVEGIGPRQAVKILSGISPEDLESAL
                     DTENLARLQQVPGLGAKTAQKLVFSLKGKLPGSAPQTVRGPWADVVQALADMGYDRKR
                     AAEAVARLSGELGAAGGPEAEQELFRRAIRELSA"
     gene            complement(4099..4587)
                     /gene="ruvC"
                     /locus_tag="P5313_08535"
     CDS             complement(4099..4587)
                     /gene="ruvC"
                     /locus_tag="P5313_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007431677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRY80448.1"
                     /translation="MDPGLASTGWGVLDANPGHIQYVAHGCIATSASTPLEDRLLDIH
                     ERIRGILDLYGPAEVAMEALYFAKNVTSALSVSEAKGVLRLTCRQAGLAVVEYGPGAI
                     KQSLVGRGRAEKAQVQEFVRMFLGLSEIPRPDHAADALAAAVCHAHNRWFADRIPKTG
                     PG"
     gene            complement(4655..5392)
                     /locus_tag="P5313_08540"
     CDS             complement(4655..5392)
                     /locus_tag="P5313_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY80449.1"
                     /translation="MSGHSKWATIKHKKGAADAKRGQMFTKLIKEISIAARMGGGDPE
                     GNPRLRTAVLKARAANMPKDNIDRAIKKGTGELEGVNYEELVYEAYAPGGVAILIEVL
                     TDNKNRAAAEVRNILTRAGGSLATSNAVLRLFNRKGVISLDGEKYTEDAVMEVALEAG
                     ADDIQNEGGELVIYTTPEAFEAVYNAVTAKQMETDGAEVSMIPESYIDVDKDAGGKIN
                     KLVEKLEECDDVQIVYHNANIPEDAEE"
     gene            complement(5767..6009)
                     /locus_tag="P5313_08545"
     CDS             complement(5767..6009)
                     /locus_tag="P5313_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80450.1"
                     /translation="MEHIYCDVCKKEVHNPVPEFSFFDFAHISVCESCRDDLNTALKF
                     TVREKSPFDFTWYDDLRMQLLKQGVQKGRIEIKRAR"
     gene            6223..7326
                     /locus_tag="P5313_08550"
     CDS             6223..7326
                     /locus_tag="P5313_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRY80451.1"
                     /translation="MIAEIGTGHGGDRTKASELLHAARESGAGCAKFQHVYAREIIHP
                     RTGAVPLPGGPVPLFDRFRSLETGPDFFADLKEQAESLGLLFLCTPFGVRSARELAEL
                     GVRAVKVASPELNHGELLDEVARMALPAILSSGVSTLGDIERAVDRIRHAAGDGPSPA
                     LLHCVTAYPAPEEDYNLRVLESLSRLLGLAVGVSDHSRDPVLVPALAVCAGASIVEKH
                     ICLSRSDPGLDDPIALPPSDFRRMSDAVRSAAADPRSAREALEREYGPGRVEAVLGDG
                     RKRLAPSERANYGRTNRSLHALRPIPRGETFTRENVAALRTEKILRPGLDPALFDVVL
                     GRRAARDVPDGEGIEWGDVGGQEPGARSQESGG"
     gene            complement(7409..9772)
                     /locus_tag="P5313_08555"
     CDS             complement(7409..9772)
                     /locus_tag="P5313_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRY80452.1"
                     /translation="MTMNERTLDLLDFRRVRDDVAGYCRSDEGRASVLGSLPGPDPGR
                     VEDAKARSRAIGALFEEGLPLPGGTFPPIAGFLPVLSKEGTCLEQEELYALALWSEAY
                     GELKNFLAKAEHPVLAGEAEAAPDLGEVPRIVFRVLARDGTLADLPELRDAQARIRRV
                     NSEIERITSSFFQDGDLRSMLQSDLPTQRDGRTVLAVRANFKAKIRGIVHEVSDTGRT
                     VFIEPEALVRKNNELVEAEAKYRQEVFRILKETTAKVAVRLEDIEAARAACAAFDDLW
                     ARARYSHLSRGTFALPGSAGLALRKARHPMLGSKAVPIDLEIPEEVRTLVITGPNTGG
                     KTVSLKTVGLLCMMNQFGLAVPAQEGTRLPVLDGVHADIGDEQSIDQSLSTFSGHMKA
                     VSGIVRDATARGLVLLDELGSGTDPEEGCAIAMSLLDHFIETGCLTIVTTHHGILKNY
                     GYTRPGVLNASAEFDKNTLSPTYRIIMGIPGESRALDVAARNGLPPAIVSGARKYLAE
                     ERTDVAAMIQGLSEKRRELEGLEEEQRRRIRAAKEEQRKADLRALQLRQREAELREQG
                     IGELKRLLAESRRGLENLVREIREGELTTEKTKAVKEFLGGLEAEVLAQEERYEEGRR
                     KAARETSGIPEAPEAALREGSPVLVGAARQRGRLIRRAKKGYWLTEVGSLRLTVPEED
                     LTACREEAAPPPKVLLDPGVGRGSKAVFELDLRGFRLAEALKAVENQVEAASLASLNL
                     FSIIHGTGEGILGRGIHELLKAHPAVRDYYFARPEEGGFGKTVVHLK"
     gene            complement(9759..10739)
                     /gene="rsgA"
                     /locus_tag="P5313_08560"
     CDS             complement(9759..10739)
                     /gene="rsgA"
                     /locus_tag="P5313_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRY80453.1"
                     /translation="MTDLVGVVVTGSNNSFLVETPDGALRRCAIKGKILRGLEGYYNS
                     LAPGDRVRLEPDAFRPDQGSITGMEARRNLFWRWNEKGKSVQAIAANLDLVACVASVR
                     LPPFRPRFVDRVAVTAELENLPLLIVLNKSDLGMDPETEARTEDYRRIGYEVLVCSAR
                     DGRGVEELRSRIRGRTTAFVGQSGAGKSSLLNALEPGLDERVGEVSEKYERGRHTTTR
                     SVMVTLGDGETRVVDTPGFRRLAIRGIPRDSLVACFPEMRPLAEACGYGLACGHLDEP
                     GCRVRGGVEEGRVHPDRYESYLRVRAELDQTVEYAKKPGKPRRVYLDDDE"
     gene            complement(10736..11533)
                     /locus_tag="P5313_08565"
     CDS             complement(10736..11533)
                     /locus_tag="P5313_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRY80454.1"
                     /translation="MFDSGVGGLPYLEALRLRIPGAPCHFAADREGFPYGRKTREEVR
                     ALVIDRVGRIADRFSPRLIVLACNTASQAALEAVRERFPEIAFVGTVPAVKPAAQSSL
                     TGTIAVLSTDRAAVDPYLDSLVDRWARGIRVLRVGAQGLVEFVEREYLDASEEERLAA
                     CRSALEPIRGEGADRVVLACTHFLHVADYIARAAGPEVRVVDSRAGVTRRAAELYLEA
                     GASIDGAPADGTSGPRRGGFYATGDPGSWTGLKPFAERYGLTFEGAL"
     gene            complement(11556..11843)
                     /locus_tag="P5313_08570"
     CDS             complement(11556..11843)
                     /locus_tag="P5313_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80455.1"
                     /translation="MCYACGTKLPARERLGFRDFCPDCGRAVHVCRNCRFYKPGAHWD
                     CAETVSELVTDKEKANFCEWFSPSPEAGAGLSKSSDAAGKARSSFDDLFKT"
     gene            11908..12510
                     /locus_tag="P5313_08575"
     CDS             11908..12510
                     /locus_tag="P5313_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRY80456.1"
                     /translation="MYSFERCGTPGCGFPALTGQGACAAHVPDPAACAADLAGRISSG
                     DTIKDLNLAGLSFEGQDFGKRHFYGCNFSRATIRNVLFTGAVFRMCFFDFAVLDSCDF
                     SGSDIQFCSFAGCRVSNSSFENSEFVHNNFNGARTRECTFNFSNLYNSRFIRSEFEST
                     DFVDCNMKKAYFLRTKESDVSFKFSNTQEAVFDIELEEES"
     gene            12507..13115
                     /locus_tag="P5313_08580"
     CDS             12507..13115
                     /locus_tag="P5313_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY80457.1"
                     /translation="MKVFFHYSLYGFSNVYLVGNEPGGDALLVDPAEFTENLLNFIES
                     NGYYVRAVLVTHNHIHHVRGLSTLLRIYDARVFSSNAEVRDMPCTVLHDGDVIDLCGM
                     RVEALSVPGHSSDSIVYKVDKLLFTGDALEAGMIGHTLSRYGNALLREGIDRRILSQS
                     EDCLILPGHGPPSTVGAERLYNLGMAEEAAADLLSDRYELFV"
     gene            13225..13755
                     /locus_tag="P5313_08585"
     CDS             13225..13755
                     /locus_tag="P5313_08585"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRY80458.1"
                     /translation="MRSSRWILALAAAAFALALAAPAFAADDVRGLWKIIDDKTGKPR
                     GYVLLYQFDGKLFGRMVATIDKETGTIKDTLDNRKEKAEKLAGDPFYAGLDFVYRLED
                     RGKEWRGQIMDPESGDEYDCRLWKEGDKLIVRGQLKGLGFLGRNQTWMRASNTELGGF
                     GVPDPQGFNPVIPRKK"
     gene            complement(13823..14677)
                     /gene="pdxY"
                     /locus_tag="P5313_08590"
     CDS             complement(13823..14677)
                     /gene="pdxY"
                     /locus_tag="P5313_08590"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HRY80459.1"
                     /translation="MSILSIQSHVAYGCAGNRAAVFPLQRLGFDVWPINTVQFSNHMG
                     YGGWEGDVFPADHIARVFRGIEERGVLPECSAVLSGFLGDPDIGGIILEAARSVKKAN
                     PKALYCCDPVMGDYDRGATVHPGIPEFLRTRAVGTADIVTPNQFEAEILSGVEIRSVG
                     DARRAADALHAEGPRLVLITSFKPDPSEEGSISLFLSGPGGPWVLTTPELPLHPPLVG
                     AGDLTAALFLGHYLRTGDPRDALERMADTVFAVLERTVRDGYRELRLVQCQDRIADPG
                     RRFPSVPA"
     gene            complement(14674..>15822)
                     /locus_tag="P5313_08595"
     CDS             complement(14674..>15822)
                     /locus_tag="P5313_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HRY80460.1"
                     /translation="GKCGYCSFHSRPLKACPDNEPERMTAALLDRARDLADRFGVSGF
                     ATAYVGGGTPTALGPDALGTLLEGIAGLGGGTPFREWTVEANPESLTAETLRVLRDAG
                     VDRLSLGFQSGQAEVLRAAGRSAGTSEGMSALELASSWRGRLSVDLMIGLPGQDPEGV
                     RTDIGRAAAAGAEHLSIYELTVEEGTPLETAVESGTVRLPPEAMEAELWSAVAETCAS
                     AGYRRYEVSNWARPGAECLHNRNYWRGGAWIGTGPSAVSSIPLPDGGTLRIQETRDHE
                     DFLSDAGAFAREEIVPPATAAFEAVMTGFRTREGLDSEAFRARFGWAPEDLLRGSFSK
                     WKSLLEKGPRGPAPSDRLLDILNPFLLDCMAEMEGRFEGRALRPGGPA"
BASE COUNT         2529 a         5500 c         5129 g         2664 t
ORIGIN      
        1 gatccgggag ccgacctggt ggctgaagcc catcggctcg gcatccgggt cgtacccctg
       61 cccggtccgt cttccattct tcaggccctg atggcgtccg ggttttccgg ccagaggttc
      121 gagttcctcg gatacctgcc gcccagggga gaggatcgca ggcaggccct gaagcgcgtg
      181 gagcgggaag cttcccggga cgggtccacg cggattttca tcgaaacccc ctaccgcaac
      241 caggccttgc tggatgacgc cctggccgtc ctggaaccgg gcacccggtt ctgtgtcgcg
      301 atatccctgg ggggggcctc cgaactcgtc cgctcgatgg agatccaccg ctggcgcgcc
      361 gcggggatcc acctggaaaa ggagcccgcc gtattcctcc tggccccgaa ggcggttccc
      421 ggcgccttga cgggccgacc ttccggcgag gccgccgtta aacgggaagg gccgagatcc
      481 ggctcggcgg cgcgacaagc ggcccggcca tcggccgatc gacacggatc tcctcgaacc
      541 ggacagacag cccgccgccg aacccggaaa aatccgggtg cttgacgcgg gcacggcttc
      601 cggtacaatg gacgaggtat ggtaaaaacc gacaaggtga acgtcagcga aacgtacgcg
      661 gaatccgtgg tctccctcag ccccttcgcc ttgaagaaac tgggtatcat atccaaccag
      721 accctcctca aggtggatac ctacaacctg gtgtgcatac cgtaccggct ttccatgaag
      781 ggggccgtac tcctggggtc cttttcccgg gacgagatcg tcttcttcca aaggttcaag
      841 ggatccctgg cgggtctcaa tctcgtcctg caacccgggg attcccccgc gccccgcaag
      901 gttttttgcc gctgttccct ggcccagttc gcccccatga aaggcaggga atccgtcggc
      961 ctcatctccg tggaattccg cccctgcccc ccggacctgg agaacctcat cggcggatat
     1021 gtgatgctga tggaccgcct ccgggcggac taccgggacc tgtccggcaa gaccgtcccc
     1081 gtcaatccgg aaaccgcgaa gatcctgggt ttcaacaact acgccaccct gaactccggc
     1141 tccatcgtga ccaagctggc cctcttcacc ctcggggcgg atcgtctggt cttcctggct
     1201 cccatgcaga ccccggatct ccagccgggg cagaccgcga cccttcgcct cttcttccag
     1261 aagtaccaat tcaacgtggc cggccggatc acggaatcga tccgcctacc cacgggcgtc
     1321 cagaaaggga ccgtggagct cgagttctcc ccggagctgg tggatctcct ggaaatgtac
     1381 tacctcgagg cgaagctggg agcccggatt ccgaaatcgg ccgtctaggt ctgcccccct
     1441 tcccggacgg tcccctacag gattaacatc gcatccccgt agctgaagaa ccggtaccgc
     1501 aggcggacag cctcctcgta ggccttcagg atcaattccc tgcccgcgaa ggcggagacc
     1561 agcatcagga gggtggattc gggggtgtgg aagttggtga acagggcgtc cacgacctgg
     1621 aagctccggc ccgggtacag gaagatccgg gtcgagctgg aaccggcccg cagggatccg
     1681 tccaaccatg cgctctccag ggtgcgcacg ctggtcgtcc cgacggccac cacgggccgc
     1741 ccctcccggc gggcccggtt cacggcctcg gctgtcgcct ccggcacgct gtactcctcc
     1801 tcgtgcatca cgtggtcctc gatccgctcc gcccggacgg gcaggaaggt acccaggccc
     1861 acgtgcaggg tcacctgggc ggtttccatc cccgcggtcc ggagggaggc caggagcccg
     1921 tccgtgaaat gtaggcccgc cgtgggggcc gccacggacc cgatggtccg ggaatacacg
     1981 gtctggtacc gttccgcgtc gaaaccgtcg tcctgcctgc ggatataggg agggaggggc
     2041 atgtgccccc accgatccag gtactcgtcg tcgatcggcc ggtccagctc caggattcgc
     2101 agatcctcct cggtgccgac gatgcgcccc tcgatcccgc ccgggaacgc gtaccgcttt
     2161 ccgggaacct gtttccgggc cttggtcgtc atggccagcc attcccggtc cccccggcgg
     2221 cggagcagga ggaattccac ccggccgccg gtaccggact cggcgtagat ccgggccttc
     2281 cgtaccttgg agtcgttgaa caccagcagg gatcccgggg ggaggtgccg ggcgaggtcc
     2341 gagacggcgg agtgcgtgaa ggtccccgta ccccggtcca ggacgaaaag ccgggatgcc
     2401 ccacgctcgc cgctcggcct ctgggcgatc aggtcttcgg gaaggtcaaa agagaagtct
     2461 ttcgtccgca tgcccgttcc ggtatcccag gtgctcgtag gccagggggg cggccacccg
     2521 gccccgggga gtcctctgga ggaaccccgc ctggatcagg tagggctcgc agtagtcctc
     2581 cagggtctcc acggattccc ccacggagat cgcgagggtc tcggcgccca cgggcccccc
     2641 gccgtacttc tcgatcaccg agcggagtat gtcccggtcg tggcgctcca ggccgtaccg
     2701 gtccacctcc aggcgcgcga gcccttcctt gaccacaccc tcggtcaccc gtccctcccc
     2761 gaaaacctgg gcgaagtcgc gcatccgccg gagcaggcgg ttggcgaccc ggggggtacc
     2821 ccggctggag gcggccagca ggtccgcggc acccgggtcc agctccatcc ccaggatggc
     2881 ggcggagcgg ttcagtatca ggatgatctc ccggggctcg tagaagccga agcgcaggga
     2941 gatcccgaag cggctgacca ggggactgga aaccattccc gccttggtgg tcgccccgac
     3001 cagggtgaag ggcggcaggg gaatccgcac cgtgcgggcg gagggtccct ggcctatgat
     3061 ccagtccagc tcgaaatcct ccatggcgat gtagagcatc tcctcgatgg cgggcttcag
     3121 gcggtggatc tcgtctatga aaaagaccgt ccgttccgtg acggtggtca ggatcccggc
     3181 caggtccttg ggcttgtcca gggccggagc gctggtcacc ttgaaatcca cacccagctc
     3241 ccggctcacg atcccggcca gggtcgtttt cccgaggccg ggaggcccca gcaggaagac
     3301 atggtccagg gcctcccccc gttcccgggc ggcccgtatg aagacggaca ggttttcctt
     3361 gaggcgttcc tggcccagga actccgaaag gcgctggggc cgaagcaccc cttccttgtc
     3421 gtcctccgcg ccccgcagcc cgggatccag gatgccccgg ggatcgcgct ccgcctcggg
     3481 gctcacgcgg acagctcccg gatggccctg cggaagagct cctgctcagc ctcgggaccg
     3541 ccggcggccc cgagctcccc ggacaaccgg gccaccgcct cggccgcccg cttgcggtca
     3601 tagcccatgt ccgccagggc ctggaccacg tcggcccagg gtccccggac ggtctggggc
     3661 gcggagccgg gcagtttccc cttcagggag aataccagct tctgggcggt cttggccccg
     3721 agtcccggga cctgctggag ccgggctagg ttctccgtgt ccagggcgga ttccaggtcc
     3781 tccggggaga tcccggacag gatcttcacc gcctgccggg gcccgatccc ctcgaccttg
     3841 aggagctcca ggaagagcgc ccgttcccgt tccgagggaa agccgaagag gcgcatctgg
     3901 tcctcccggt gcaggaggtg gatgtagacc ttggtccgtc cgccgagggt tccgaactcg
     3961 tcgatggatc gggcgggcac cgagaattcc cattcaaggc cgttgttgtc gacccggagg
     4021 atgtcgaacc gtttttccgt gatcgtcccg aggagggaat tgaacatgcc gggagtatac
     4081 cccggcgggc cgggcgcttc aacctggccc ggtttttgga atccgatcgg cgaaccagcg
     4141 gttgtgggca tggcacacgg cggcggccag ggcgtccgcg gcgtgatcgg gccggggaat
     4201 ttccgagagc ccgaggaaca tccggacgaa ttcctggacc tgggcttttt ctgcccggcc
     4261 ccgccccacc agggactgct tgatggcacc ggggccgtac tccacgaccg ccagtcccgc
     4321 ctggcggcag gtcaggcgca ggaccccctt ggcctcggag acggacaggg cgctggtcac
     4381 gttcttcgcg aagtagaggg cctccatggc cacctccgcg ggtccgtaga ggtccaggat
     4441 cccgcggatc ctctcgtgga tgtccaggag ccggtcctcc aggggggtgg aggcggaggt
     4501 agcgatacag ccgtgggcta cgtactggat atggccggga ttcgcgtcca ggacgcccca
     4561 gccggtggaa gccagtcccg ggtccaagcc caggatgcgg accctccctc cctcggccgg
     4621 gaccggaggg agggatcgga ccgccgcgcc gggatcactc ctcggcgtct tcggggatgt
     4681 tcgcgttgtg gtagacgatc tggacgtcgt cgcactcctc gagcttctcg acgagcttgt
     4741 tgatcttacc gcccgcgtcc ttgtccacgt cgatgtagct ttcgggaatc atggaaactt
     4801 cggcgccgtc ggtctccatc tgcttggcgg tcacggcgtt gtacacggcc tcgaaggctt
     4861 ccggggtggt gtagatcacc agctccccgc cctcgttctg gatgtcgtcc gctccggcct
     4921 ccagggcgac ctccatgacc gcgtcctcgg tgtacttctc gccgtccagg gagatcacgc
     4981 ccttgcggtt gaacaggcgg aggaccgcgt tggaagtggc cagggatcct cccgcccggg
     5041 tcaggatgtt gcgtacttcc gcggcggccc ggttcttgtt gtccgtgagg acctcgatca
     5101 ggatggcgac tccgccgggg gcgtaggcct cgtacacgag ttcctcgtag ttgactccct
     5161 cgagttcgcc ggtgcccttc ttgatggccc ggtcgatgtt gtctttgggc atgttggcgg
     5221 cacgggcctt gagcaccgcg gtgcggagac gggggttgcc ctcgggatcg ccgccgccca
     5281 tgcgggccgc gatggagatt tccttgatga gcttggtgaa catctggccg cgcttcgcgt
     5341 ccgcggctcc cttcttgtgc ttgatcgtcg cccatttgct atggccggac atattggctc
     5401 cttatacgaa tggaatttca gccgattccg gccggaacta tgcggccggg ccgaattgga
     5461 cgggtacccg acgggctccc gggcaaacct gcggttgttc ggctatcgcg ggagcggcgg
     5521 agggtgaagt ctccggtaaa accatatcat ataatgagga acgatgtcaa gaaatggaga
     5581 agttcgacat atccggccag aacgagcggt atcgtacgct cccatgccgt gcaggatact
     5641 cagaggctta cccgccgtcc cggaacgcag acccgtacgg gcgcggggtc atgtcgatgg
     5701 gaaccttcgt gatctccccg tctcggcatc atgcccgcgc ccctcgaaat tccggtgaat
     5761 acgggcctat cgggcgcgct tgatctcgat ccggcccttc tggacgccct gcttgagcag
     5821 ctgcatccgg aggtcgtcgt accaggtgaa gtcgaagggg gacttctcac gaaccgtgaa
     5881 cttcaacgcc gtgttcaggt cgtcccggca agactcgcag acgctgatgt gggcgaaatc
     5941 gaaaaaagag aattcgggaa cgggattgtg gacttccttc ttgcaaacgt cgcagtagat
     6001 gtgttccatc cttgcatctc cttgtataca ccccgggtcc gcgctcggcg gactccgcat
     6061 gccaagagat tccggcgcct gaaggcgtcc cggctttcag ggaaacagtt tatatgatag
     6121 cgtggcggtt gtcgagtgtg gatggaaaaa agcaaggggg agccggtggt atcctacagc
     6181 ttcgtcgttg agggagttgc ctatcatccc gggaacccgc tcgtgatcgc ggaaatcggg
     6241 accggccacg ggggggatcg gacgaaggcc tcggaactgc tccacgccgc ccgggaatcc
     6301 ggcgccggat gcgcgaagtt ccagcacgtc tacgcccggg agatcatcca ccccaggacc
     6361 ggagccgtcc cccttccggg cggaccggtt cccctcttcg accggttccg gtccctggaa
     6421 accggcccgg acttcttcgc ggacctgaag gagcaggcgg aatccctggg actgttgttc
     6481 ctttgcactc ccttcggggt gcgaagcgcc cgggaactcg cggagctcgg ggtccgggcc
     6541 gtcaaggtcg ccagccccga gctcaaccac ggggagctcc tggacgaggt cgcccgcatg
     6601 gccctccccg ccatcctgtc ctccggggtt tccaccctgg gggacatcga gcgggccgtg
     6661 gaccggatcc ggcatgcagc cggggacgga ccgtccccgg ccctcctcca ctgcgtgacc
     6721 gcctaccccg cgcccgagga ggactacaac cttcgggtcc tggaatccct ttcgcggctc
     6781 ctgggccttg ccgtgggcgt ctccgatcac tcccgggatc ccgtcctggt cccggccctg
     6841 gcggtctgcg ccggggcctc gatcgtggaa aagcacatct gcctgtcccg atcggacccg
     6901 ggcctcgacg accccatcgc ccttcccccc tccgacttcc gccggatgag cgacgcggtc
     6961 cggtccgccg cggcggaccc gcggtccgcc cgggaagccc tggaacggga gtatggaccg
     7021 ggcagggtgg aagcggtcct cggggacggc cggaaacggc tggcccccag cgagcgggcc
     7081 aactacggcc ggacgaaccg gagcctccac gccctgcggc ctatcccccg gggcgagacc
     7141 ttcacccggg aaaacgtggc cgccctcagg acggaaaaaa tcctgcgacc cggccttgac
     7201 ccggcgctgt tcgacgtcgt gctgggccgc cgggccgccc gggacgttcc ggacggggaa
     7261 gggatcgagt ggggggatgt gggtggacag gagccaggag ccaggagcca ggagtcaggg
     7321 ggctgacaaa tcgcacgcac aacgaatcgg tgctcgcagg tttatatctc tttccccctc
     7381 tcgtctgtcg cttttctcct gattccttct atttgaggtg caccaccgtc ttgccgaagc
     7441 ctccctcctc ggggcgggcg aagtagtagt ccctcaccgc ggggtgcgcc ttcaggagct
     7501 cgtggatgcc ccggcccagg atcccctccc cggtgccgtg gatgatggag aacaggttca
     7561 gggatgccag gctcgcggcc tccacctggt tctccacggc cttcagggcc tcggccaggc
     7621 ggaacccccg gagatccagc tcgaacacgg ccttggagcc cctgcccacc ccgggatcca
     7681 gcagcacctt gggcggggga gccgcctcct cccggcacgc ggtcagatcc tcctccggga
     7741 cggtcaggcg taaagagccc acctcggtca gccagtatcc cttcttggcc ctgcggatga
     7801 gccggccgcg ctggcgggca gcgccgacca ggaccggaga gccttccctc agcgcggctt
     7861 ccggagcttc cgggatgccg gaggtctccc gggccgcctt cctgcggccc tcctcgtagc
     7921 gctcctcctg ggccaggact tccgcctcca gaccccccag gaattccttg accgccttcg
     7981 tcttctcggt ggtcagctcc ccctcccgga tctcccgcac caggttttcc agcccccggc
     8041 ggctctcggc caggaggcgc ttgagctccc cgatgccctg ctcccggagc tccgcctccc
     8101 tctggcggag ctggagggcc cgtaggtccg ccttcctctg ctcctccttg gccgcccgga
     8161 tccgccgcct ctgctcctcc tccagaccct ccagctccct ccgcttttcc gacaggccct
     8221 ggatcatggc ggccacgtcg gtccgctcct cggccaggta cttccgggca ccggagacga
     8281 tggccggggg caggccgttc cgggcggcca cgtccagagc gcggctctcc ccggggatgc
     8341 ccatgatgat ccggtaagtc ggggacaggg tgttcttgtc gaattccgcg gaggcgttca
     8401 ggaccccggg ccgggtatag ccgtagttct tgaggatccc gtggtgggtg gtcacgatgg
     8461 tcaggcagcc ggtctcgatg aagtggtcca acagggacat ggcgatggcg cagccttcct
     8521 cgggatccgt gccgcttccc agctcgtcca ggagcaccag gccccgggcc gtcgcgtccc
     8581 ggacgatccc ggataccgcc ttcatgtgcc ccgagaaggt ggacagggac tggtcgatgg
     8641 actgctcgtc cccgatgtcc gcgtgcaccc cgtccaggac gggcaggcgg gtaccctcct
     8701 gggccggaac cgccaggccg aactggttca tcatgcacag gagccccacg gtcttgaggc
     8761 tgaccgtctt gcccccggta ttggggcccg tgatcaccag ggtccggacc tcctcgggta
     8821 tctccaggtc gatgggcacc gccttggagc ccagcatggg atgccgggcc ttcctcaggg
     8881 ccagacccgc ggatcccggc agggcgaagg tcccccggga gagatgggag taccgggccc
     8941 gggcccagag gtcgtcgaag gccgcgcagg ccgccctcgc ggcctcgata tcctccaacc
     9001 ggaccgcgac cttcgcggtc gtctccttca ggatccggaa gacctcctgg cggtactttg
     9061 cctcggcctc cacgagctcg ttgttcttgc ggaccagggc ctcgggctcg atgaagaccg
     9121 tgcggcccgt gtcggacacc tcgtggacga tgccgcggat cttggccttg aagttggccc
     9181 gaaccgccag gacggtgcgc ccgtcccgtt gggtgggaag gtccgactgg agcatggacc
     9241 gcaagtcccc gtcctggaag aaggaggagg tgatccgctc gatctcggaa ttgaccctgc
     9301 gtatccgggc ctgggcatcc cggagctccg gcaggtccgc cagggtgccg tcccgggcca
     9361 gcacgcggaa gacgatccgg ggcacctccc ccaggtccgg ggccgcctcg gcctcgccgg
     9421 ccaggacggg gtgttccgcc ttcgcgagga agttcttcag ctccccgtag gcctcggacc
     9481 agagggccag ggcgtagagc tcctcctgct ccaggcaggt tccttccttg gacagtaccg
     9541 gcaggaagcc cgcgatcggg gggaaggtgc cgcccggcag gggaaggccc tcctcgaaaa
     9601 gggcgccgat cgcccgggag cgggccttgg catcctccac gcggcccggg tccggtccgg
     9661 gcagggaccc gaggacggaa gcccgtccct cgtcggaacg gcaatagccc gccacgtcgt
     9721 cccggacgcg gcggaagtcg aggagatcca gggtgcgctc attcatcgtc atcaaggtag
     9781 accctccggg gttttcccgg tttcttcgcg tattccacgg tctggtccag ctccgcccgc
     9841 acccggagat aactctcgta gcggtccgga tggacccggc cctcctccac cccgccgcgg
     9901 acccggcagc ccggctcgtc cagatgcccg caggccaggc cgtacccgca ggcctcggcc
     9961 aggggccgca tctccgggaa acaggccacc agggagtccc gggggatacc gcggatcgcc
    10021 agccgacgga aacccggggt gtccacgacg cgggtctctc cgtcccccag ggtgaccatc
    10081 acggaccggg tcgtggtatg ccggccgcgc tcgtacttct cggagacctc cccgaccctc
    10141 tcgtccaggc ccggctccag ggcgttcagg agcgagctct ttccggcccc cgactgcccc
    10201 acgaaggccg tggtcctgcc gcggatcctg gaccggagct cttcgacacc ccgtccgtcc
    10261 cgggccgagc agaccaagac ctcgtagcct atgcggcggt aatcctccgt ccgggcttcg
    10321 gtctccgggt ccatccccag gtcggatttg ttcagcacga tcaggagcgg caggttctcg
    10381 agttccgcgg tcacggccac ccggtccacg aagcggggcc ggaagggcgg cagccggacg
    10441 gaggccacgc aggcgaccag gtccaggttg gccgcgatgg cctgcaccga ctttcccttc
    10501 tcgttccagc gccagaacag gttgcgccgg gcctccatgc ccgtgatgga cccctggtcc
    10561 ggccggaagg cgtcgggttc caggcggacc cggtccccgg gggccaggga attgtagtac
    10621 ccctccaggc cgcggaggat ctttcccttg atggcgcagc gccgcagggc tccgtccggg
    10681 gtctccacca ggaaggagtt gttcgatccg gtgacgacga cgccgaccag gtccgtcaca
    10741 gggctccctc gaaggtaagt ccgtagcgct ccgcgaaggg tttcagcccg gtccaggatc
    10801 cgggatctcc ggtggcgtag aatccgcccc gacggggccc ggaggtcccg tccgccggcg
    10861 ccccgtcgat cgaggccccg gcctccaggt acagctccgc cgccctccgg gtgacccccg
    10921 cccgggaatc cacgacccgg acctcgggcc ccgccgcccg ggcgatgtag tccgcgacgt
    10981 gtaggaaatg ggtgcaggcc aggacgaccc ggtccgctcc ctccccgcgg atcggctcca
    11041 gggcggaccg gcaggccgcc aaccgctcct cctcggaggc gtccagatac tccctctcca
    11101 cgaattccac gagtccctgc gcccccaccc gcaggacccg gattccccgg gcccagcggt
    11161 ccaccaggga atcgaggtag ggatccacgg cggcacggtc tgtggacagg acggcgatgg
    11221 ttccggtcag ggaggactgg gccgcgggct tgaccgcggg caccgtgccc acgaaggcta
    11281 tctccgggaa gcgctcccga acggcttcca gggccgcctg gctggcggtg ttgcaggcca
    11341 ggacgatcag gcggggggag aaccggtcgg cgatccgccc cacccggtcg atcaccaggg
    11401 cacggacctc ctcccgggtc ttgcgcccgt aggggaatcc ctcccggtcc gcagcgaaat
    11461 ggcagggggc cccgggaatc cggagtcgga gggcttcaag atacggaagc cctccgactc
    11521 ccgaatcgaa cacgaaaacc cgattggacg gcatgtcagg tcttgaagag gtcgtcgaag
    11581 gaggagcggg ccttcccggc cgcgtccgag gacttggaga gccccgctcc ggcttccggc
    11641 gaaggggaga accactcgca gaagttggcc ttctccttgt ccgtgacaag ctcgctaacg
    11701 gtctccgcgc agtcccaatg ggcgccgggt ttgtagaagc ggcagttccg gcagacgtgg
    11761 acggcccggc cgcagtccgg gcagaaatcc cggaatccga gccgttcccg ggcaggcagc
    11821 ttcgtcccgc aggcgtagca catggacgca tgctaacccg gattcccgag gcttggcaat
    11881 gccgccgctt cggtataccc ttccggcatg tattccttcg aacgctgcgg aacccccggc
    11941 tgcggtttcc cggccttgac cggacagggc gcctgcgccg cccacgttcc cgatccagcg
    12001 gcctgcgccg cggacctggc gggcaggata agctcgggag acaccatcaa ggatctcaac
    12061 ctggccgggc tttccttcga ggggcaggac ttcggaaagc gccatttcta cggatgcaac
    12121 ttttcccggg ccacgatccg gaacgtgctc ttcaccgggg cggtgttccg tatgtgcttc
    12181 ttcgatttcg ccgtcctgga ttcctgcgat ttttcgggat ccgacatcca gttctgttcc
    12241 ttcgcgggct gtcgggtgtc caattcgtcc ttcgaaaact cggagttcgt ccacaacaac
    12301 ttcaacgggg cccggacccg tgaatgcacc ttcaatttca gcaacctcta caattcccgg
    12361 ttcatccgct ccgagttcga atccacggac ttcgtggatt gcaacatgaa gaaagcctat
    12421 ttcctccgga ccaaggagag cgacgtctcc ttcaagttct ccaacaccca ggaggcggtg
    12481 ttcgacatcg aattggagga ggaatcgtga aagtattctt ccactactcc ctgtacggat
    12541 tctccaacgt ctacctcgtg gggaacgaac ccggcgggga cgccctgctc gtggatcccg
    12601 cggagttcac ggaaaatctc ctcaacttca tcgagtccaa cgggtactac gtccgcgccg
    12661 ttctggtgac ccacaaccac atccaccatg tccggggact ttccaccctg ctccggatct
    12721 acgacgcccg ggtgttttcc agcaacgccg aggtccgcga catgccctgc acggtcctcc
    12781 acgacggcga cgtgatcgac ctgtgcggca tgcgggtgga agccctatcc gtccccggcc
    12841 attcctcgga ttccatcgtc tacaaggtgg acaagctcct gttcaccggg gacgccctgg
    12901 aggccggtat gatcggccat accctgagcc gatacggcaa cgccctgctc cgggagggca
    12961 tcgaccggcg gatcctgtca caatccgagg actgcctcat cctgcccgga cacgggccgc
    13021 ccagcaccgt gggggccgag cgtctctata acctgggcat ggccgaggag gctgcggcgg
    13081 acctcctttc ggaccgctac gagctcttcg tctgagtccc ggaaccgtag ttccagcttt
    13141 acttttagct tcccgggtcc tatactcggc cgcaacccgt aaaggccgac atttcggacc
    13201 tttatgtcat cgaaggagaa tcccatgcgc agctcccgct ggatcctggc cttggccgcg
    13261 gccgcgttcg ccctggccct cgccgccccc gctttcgccg cggatgacgt ccgcggcctg
    13321 tggaagatca tcgacgacaa gaccggaaag ccccggggct acgtcctcct gtaccaattc
    13381 gacggaaagc ttttcggacg gatggtggcg accatcgaca aggagacggg aaccatcaag
    13441 gacaccctgg acaaccggaa ggagaaggcc gaaaagctgg cgggggatcc cttctacgcg
    13501 ggcctcgact tcgtgtaccg cctggaagac aggggcaagg aatggcgcgg ccagatcatg
    13561 gaccccgaat ccggggacga atacgattgc cgtctctgga aggaagggga caagctcatc
    13621 gtgcggggcc agctgaaggg cctgggcttc ctgggccgga accagacctg gatgcgcgcc
    13681 tcgaataccg aactgggcgg attcggagtc ccggatcccc agggcttcaa ccccgtgatc
    13741 ccccggaaga agtagccgcc ccggggccgg tcggagcctc cggccggccc gcggaagggc
    13801 gggcttcggg aagccgatcc cgtcaagccg ggaccgaggg gaacctcctg ccgggatccg
    13861 ctatacggtc ctggcattga accagccgga gctcccggta cccgtcccgg actgtgcgtt
    13921 ccaggacggc gaaaaccgtg tccgccatcc gttccagcgc gtcccggggg tcgccggtcc
    13981 ggaggtaatg ccccaggaag agggcggcgg tcaggtcccc tgcccccacc aggggcgggt
    14041 gcaggggtag ctccggggtc gtcagaaccc aggggccccc ggggccgctc aggaagaggg
    14101 agatggaccc ttcctccgac gggtccggct tgaagctcgt gatcaggacc agccggggtc
    14161 cctccgcatg gagggcatcc gcggcccgtc tcgcgtcccc gacgctccga atctcgactc
    14221 ccgagaggat ttccgcctcg aactggttcg gggtgacgat gtccgcggtc cccacggccc
    14281 gggtccgaag gaactccggg atgcctggat ggaccgtcgc cccccgatca tagtctccca
    14341 tcaccggatc gcagcagtac aaggccttcg ggttggcctt cttcacggac cgggcggctt
    14401 ccaggatgat ccccccgata tcggggtccc ccaggaatcc ggagaggacg gcggagcatt
    14461 ccggcaggac tccccgctcc tcgatccccc ggaaaacccg ggcgatatgg tccgcgggga
    14521 acacgtcccc ctcccatccc ccgtacccca tgtggttgga gaactgcacg gtgttgatgg
    14581 gccagacgtc gaatcccagg cgctggaggg gaaagaccgc agcccggttc cccgcgcatc
    14641 cgtaggccac gtgggactga atggaaagta tgctcaagcc ggacctcccg ggcggagcgc
    14701 ccgcccctcg aaccttccct ccatctccgc catgcaatcc aggaggaagg gattcagaat
    14761 atccagcagc ctgtccgaag gggcgggtcc ccggggtccc ttctccagta gggacttcca
    14821 cttggagaag cttccccgca gcaggtcctc cggcgcccac ccgaagcggg cgcggaaggc
    14881 ttcggaatcc aggccctccc gggtccggaa acccgtcatg accgcctcga aggcagcggt
    14941 cgcgggaggg acgatctcct cccgcgcgaa agccccggcg tccgagagga agtcttcatg
    15001 gtcccgggtc tcctggatcc gcagggtgcc gccgtccggc aggggaatcg aggacaccgc
    15061 ggagggaccc gtcccgatcc acgcgccgcc cctccagtag ttccggttgt gcaggcactc
    15121 ggcaccgggg cgcgcccaat tcgagacctc gtagcgccgg tatcccgcgg aggcgcaggt
    15181 ctccgcgacc gcggaccaga gctccgcctc catagcttcg ggaggcagcc ggacggtgcc
    15241 gctttcgacc gccgtctcca ggggggtccc ctcctccacc gtgagctcgt agatcgaaag
    15301 gtgctccgcc cccgccgcgg ccgcccgccc gatgtccgtc cggaccccct cggggtcctg
    15361 gcccggaagt ccgatcatca ggtccacgga gaggcgcccc ctccaggacg acgcaagctc
    15421 cagggcgctc atcccctcgg aggtaccggc gctccggccc gcggcccgca ggacctccgc
    15481 ctggccgctc tggaagccca ggctgagccg gtccaccccc gcgtcccgaa gcacccggag
    15541 cgtctcggcg gtgaggctct cgggattggc ctccacggtc cactcccgga agggagttcc
    15601 cccgcccagc ccggcgatcc cctccagcag cgtgcccagg gcgtcggggc ccagggcggt
    15661 cggagtcccg ccccccacgt aggccgtggc gaagcccgaa acgccgaacc gatccgccag
    15721 gtcccgcgcc cggtccagca aggccgccgt catcctctcc ggttcgttgt cgggacaggc
    15781 tttcaggggc cgcgaatgga aggaacagta cccgcatttc cc
//
LOCUS       DAOTEI010000073        15363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2632_length_15363_cov_5.901751, whole genome shotgun sequence.
ACCESSION   DAOTEI010000073 DAOTEI010000000
VERSION     DAOTEI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15363
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2632_length_15363_cov_5.901751"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="P5313_08600"
     CDS             <1..601
                     /locus_tag="P5313_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRY80461.1"
                     /translation="ALEGMPALAKSLGLRRMSLNLFIPTARSPEADALFVPYREIGPV
                     VDRVRKRALEAGVEFLWYSPTPMCLYNPLARGLGNKNCAACDGLLSVNPRGDVLPCSS
                     WDEGVGNLLSDGFEKVWFGDRAAALKNKEYAPAECRTCPAFAACQAACPLYWSYAGYG
                     EIEEAGPAFRARHARPASSAGPEATGEPARGGTDERPVP"
     gene            582..1481
                     /locus_tag="P5313_08605"
     CDS             582..1481
                     /locus_tag="P5313_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80462.1"
                     /translation="MSDLSLKPTVTAGFRFFRMGPAARLILTGALFALGAALQVGVGV
                     LPGTLVILAGWIPLGLRRVTNKPKDQGLEKWKPVTAAEVDRILDAFSRAGKLRRASGG
                     RSALRILAGVLLMISVPVSFGISGGAGLVALDALLFLFPALFFGKVKVFTPGLIAQKL
                     LGFEAIRSEPLPEGWVLTPYIRFDEDEEGRDVPEDLRFLLEPKKKIGDLVGVQFQTAI
                     NNGANGPVPYMYAVVLTRGRKGPAYEALRGFSARGYEVERGGDDEYGTVVIRQSTGGG
                     GYHTKPDQCRKLFRLAASALARG"
     gene            1491..2093
                     /locus_tag="P5313_08610"
     CDS             1491..2093
                     /locus_tag="P5313_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY80463.1"
                     /translation="MSGELVAFLSRYIPVSGDLERIFRENDFVRTFPKGSVLLRPEDR
                     AREAYFILKGCVRSYIVKDAEERTVDFFVEEDAVLPLGYGREEPAGHYLECLEDTVAV
                     ASDPDREREMLARYPELKAVCLAMSEIMAAKLQENLARYRTSTPEERYRDLAARRPDL
                     LRRIPQYRIAEYLGIRPESLSRIRRRLARPRRPGGSSDRS"
     gene            2208..2639
                     /locus_tag="P5313_08615"
     CDS             2208..2639
                     /locus_tag="P5313_08615"
                     /inference="COORDINATES: protein motif:HMM:NF039408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HRY80464.1"
                     /translation="MNATTDARFLDVKADPKVVLALAWTALLGLYIYCDIYSLFRPGI
                     LEEMRAGKMGPFDVSQTSLALASLLMILPILAAPASVLLPARAGRILNLLLAPLYLLV
                     NIGNLLGEAWVYYYLFGILELALTAGIFLVALKWPKERFNP"
     gene            complement(2908..3810)
                     /locus_tag="P5313_08620"
     CDS             complement(2908..3810)
                     /locus_tag="P5313_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY80465.1"
                     /translation="MKLRLSRFTGDPSHEYASLIGERRQLRIRHCHDYFELFLVNRGT
                     AHHLVNGSVQRLKPGSIAFIRPDDSHSYDEMSGDFEIINVLIPCETVQALFDYLGEGF
                     DPGRLLSCPLPPLAQVAQREHEAIVQELEQLILSRKIRRTSSDSLFRITLLHIIVSCF
                     PILPDKRKSETPVWLRWLVLEMMKKENFTEGLPAMKRLAGKSEEHISRSCRKHLRKSP
                     TELINDFRLEHAARAIASTNEKIVDISSDVGFESLSHFYHLFKKAYGVSPLGFRRAAK
                     EKPEEELLFGALPLETSIPKGQAL"
     gene            3883..6144
                     /locus_tag="P5313_08625"
     CDS             3883..6144
                     /locus_tag="P5313_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRY80466.1"
                     /translation="MNNFKVKSPRKDLRFEIAFTKAYRDAIDRYTHPAAIEAACLRAQ
                     FPAILHPIQDEDLLAGRIQMGVVGLGIQHQTGGFGFYANEEKLVAELEFKAGDARYRE
                     DLHDLLTFWKGRTTNAVVLREMPESIREALGSDQWRHLPLPASPILRMAGAYVDFDKL
                     VRVGLPGLRKEVQEYKARAEAGGGDVELFAAMADVLDLVGEVCAFYQAQALDLAAKAG
                     DGKRKAELGLMSAALKSIQTAPPASLREALQLAWIYGIMAPLIEFGRLDEYAGDFFVR
                     DLESGVITEEEAVDLVSSLYRLIDHLDCETDGRVIVGGYGRRNPGNADRFCLVAVEAC
                     RRVKEVLPQFTLRFNKDTPKPVWEASMRCIEEGRTYPLLYNDDILVPGVMKAFGVDRA
                     RAETYMPLGCGEIEFDHYSFGTPSGSMNVLKILELTVRGGYEPMTGSRRGPATKPLAA
                     CATYEEFLANYKRQLDYYIEAQAVFEKYEYEITGRMHPFMMVTLLYDGCLERGKAVFN
                     GGCAYLGGTLELYGNVNAANSLAAIKRLVFEEKKISAERLVEAMDRNFVGREAEMKLL
                     RDAPKYGNDDDYVDSIFVDLHDYLSDRIREQAPKVGLDSYLAVTINNAQNTTLGRWVG
                     ATPDGRKAGMPMANANNPAPGTDRNGVTAMLNSILKPRHDNHAGMVSNIRFTRELFTA
                     SRDKMHGLVRAYFDRGAAHAMITVVGKDDLRKAMERPEEYRDLIVRVGGFSARFVDLK
                     KDVQQEIYDRVTY"
     gene            6125..7060
                     /locus_tag="P5313_08630"
     CDS             6125..7060
                     /locus_tag="P5313_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRY80467.1"
                     /translation="MTASPTDGPTAVVYDIQRLSLNDGPGIRTTVFLKGCPLRCAWCH
                     NPESWEREPRLAYTESLCRLCGECVRVCPTGAHTLEGSADSGLRHRIDWSTCDACGKC
                     VEACPHDALSLCGREYTVPELYDALRPDLPYFRIGEGGGVTFSGGEPMVAVDFLLEFL
                     RRKGSLHVCLETSGQAPAEDFRRIAPMVDLFLFDYKATGAERHRALCGEGGERILENL
                     RLLCRSGARVLLRLPLVPGINDQEEHLRAVATLLDELPGLVGAQILPYHSLGAGKEAR
                     FGLEGRTLRLPSASPEQGTAWLKSLESFGARKVFL"
     gene            7110..8351
                     /locus_tag="P5313_08635"
     CDS             7110..8351
                     /locus_tag="P5313_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY80468.1"
                     /translation="MKRMGIAILALVALALLFAPAAAAQDKKVNLLVSRWAGPHADFQ
                     KQMVKEFPDAAVRIDDIDYGSLKQKQMTSFQAAKGTGNYDVVWVNIQWMKEYVDAGYL
                     LPIDDLLKKYKVDTKIYAKGMMQGAVIDGKTYGLPTFAQCLILAYDSAAFDAAKLKVP
                     RTADDLVAVAKYFKETKGSGIAIPAKQGGAAATLYSQLLFSSGGFYFDKKGKLDLLSE
                     PSVYAATIYDQLARYSVRGATAWHHDETAEAVRTKTAPIGIIMSGLANQNHDPEKSLI
                     VDTVKYATLNGKTGDTAANNAYWVWAIAKNTNDVDASFKFISWMTSPAIEKKQTLANK
                     QISAITSLSEDPEVQKAMPYLSVVMKELANGKMDPALKNFQTLKNALIVGLSEIATTD
                     AKPEDVLKKIQADLAATDFSK"
     gene            8461..9366
                     /locus_tag="P5313_08640"
     CDS             8461..9366
                     /locus_tag="P5313_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY80469.1"
                     /translation="MRKPRALSLEKGDLLPTLLMAPAVSLVLGVMVVPLAFGAFLSFF
                     DFSFGSFDPRTAFVGLKHYLRFFRDGTALKALLNTLMFSAGAIAGDFTIGTLAAVLIH
                     SLSRRLARFVRPIVTVPLLVSPIVVGLIWRYIYDPQGILYWMMDRVGIPFEFFPGVTS
                     ASTALLSTIIAHWWQVVPFLIIVLTAGLVSIPTELYEAARVDGAGALSIFFRITVPLL
                     RDVYLVILLISGVDTIKVFDIIFALTGGGPNNSTVSLSIYAYTQGFENGDLSYGIAVS
                     MVAMIATFGIFGIPFILQNRKRRGS"
     gene            9366..10214
                     /locus_tag="P5313_08645"
     CDS             9366..10214
                     /locus_tag="P5313_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY80470.1"
                     /translation="MTRNTRMIAPSKWPPRLAKAFFLLVFGLWTAAPVYIIVSNAFRR
                     TLDIKKMPPDLIFKPIFTHFQRLFELDDFLRYFVNSIVISVSVTVTTIALGTLAAYGL
                     KLFKSRLGERLSNLLLIGKLVPAITVLLPMFILMNRLRLTGTYLGPILAHSAMGLPFV
                     TWIMASFVRDIPGEILDSATVEGCSRMKAFSRIIFPMLTPAIASAVILIMQFSWNELL
                     FSLQLTNLDTYPLTVGIARYVGAISVDWGKCSAAATISIAPIIVVGFLMQKYLVTGMT
                     AGAVKG"
     gene            10242..12437
                     /locus_tag="P5313_08650"
     CDS             10242..12437
                     /locus_tag="P5313_08650"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRY80471.1"
                     /translation="MIFGAQYYRPPFPGRECWDRDLANMADLGFNTVKLWAVWNWIEP
                     VRGRFDFSDLDELCGLAERRGLAVVINTIPEGSPPWVLPGNEDALYRTGRGEVIPFGG
                     PPNLPSAGWPGLCMDKPAAADLTARFIEAVAARYRTARSVVALDVWNEPHLEPMFDYR
                     GELLCYCEHSRAAFRRWLKDKYGDLGSLNAAWFRRLSDWSEVEPPPRFGTWADMLDWR
                     LFWIENLARHLRIRTEAARRGAPGTAIQTHVAYSAVLGNKLAGGLANELGDEFSLARE
                     VDVFGLSSFPKWLQGKNHPFTHLAHNEVVAEAARGKRYYQVELQGGGGKAGFLGGEVP
                     TARDVRIWNYNTAAAGGKGVLYWQYAPEPAGLESPGFGLTAYDGGNTERSREAGACAR
                     ELSDPRLDAATRVLPVNAIYLSRKSQVLSFCAERREDLYAGSVAGIYRAAYEAGIPVR
                     FVHEDYLESLGVEGVRTLFVPMALCLDDRERAALRAFAEAGGLLVGEAATGMYDSGGA
                     LDPDSRALADLFGVRHVELEALPDWGFTEALGDGFSFGGAQYRRVVEPGPGTEVLARF
                     PDGRPAATGRALGAGRAVYVATFAALAFHLRGDPGTRNFLAGFLDPAGYPQLRRLAVL
                     PGEEPERAEGAAAAPSSAATRAAAVSASSAPAAAPGAPAAPAAFVPSAVVRLLETPED
                     WVLVAVNHREGPARIELDFDSALVREASASLTVPGGSGTVRYFRKAGRT"
     gene            12531..14228
                     /locus_tag="P5313_08655"
     CDS             12531..14228
                     /locus_tag="P5313_08655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRY80472.1"
                     /translation="MKKFKDIPYARPDIEAESAAFRSLLADFSGARSAEAAREAMDRL
                     SALRDRISTVKDLAEIRHTCDTTDPFYEAENDFWDGASPRIDALEHELYAALVSSPFR
                     KELEAVWGPHIFRMAECRLAAFKPGIEELLAAENRLSSEYDKLKASASIPFDGRVLNL
                     AELGPYMESPDRETRGRAVSAVSGFYRDHGDEFDRIYAELVAVRHRIALELGFPNFVP
                     LAYARLGRTDYGPAEVASYRSQVLRTVVPLAASLRERQARRLGLPVLAYRDEAFEFPT
                     GNPRPVGGTPVLLEAAGKMYDEMSAETGSFFRMMRERELFDLETRKGKALGGYCSYLP
                     AEGVPFVFANNNGTSDDIDTLTHEVGHAFQVSRGGRFAFPEYHWPTLEACEIHSMSME
                     FLAWPWMEGFFGADADKYLFRHLTAGILFLPYGVLVDEFQHWVYEHPGAAPAERRAAW
                     RRMERVYLPWRDYEGDGFLEAGGYWFRQGHIFQDPFYYIDYTLAQVCAYEFWALSRSD
                     RPGAWRRYLALCDAAGSRPFLDLLGVASLRNPFAEGSIAGILAPARTWMDAVDDRGF"
     gene            14421..15089
                     /locus_tag="P5313_08660"
     CDS             14421..15089
                     /locus_tag="P5313_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80473.1"
                     /translation="MVERVRESVRGSGPVRNPRLPASASRSLPRGVLLALVLLAFADP
                     GGAQVTGLSGIRFESPIPVPVYLLDRTIAIPEAVPGYSEPDYIFRAEGGNRTLRALDL
                     YRYLGDSPVALDLTPGAYTFQLVLPGNEVRTIQVDTREPGDRTQTWEIRPGREGANVL
                     GLTLSLAGGMVWWGAVSLAVREWFVEDEFGAASIVWASSHALFIGGLVIAFGPGSPRI
                     RRIE"
     gene            15159..>15363
                     /locus_tag="P5313_08665"
     CDS             15159..>15363
                     /locus_tag="P5313_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY80474.1"
                     /translation="MRFRKLCCSCILILLSAASATAQIDPLVEAVRRGSLDDIRKILE
                     SGADVNERNAGGMTALMAVARDGD"
BASE COUNT         2456 a         5199 c         5085 g         2623 t
ORIGIN      
        1 cgccctggag gggatgcccg ccctggccaa gtccctgggg ctgcgacgga tgtccctcaa
       61 cctcttcatc cccaccgccc gcagtcccga ggcggacgct ctgttcgtgc cctaccggga
      121 gataggcccc gtggtggacc gggtccggaa gcgggccttg gaggcggggg tggagttcct
      181 ctggtactcc ccgacgccga tgtgcctcta caaccccctg gcccgagggc tggggaacaa
      241 gaactgtgcc gcctgcgacg gccttctttc cgtgaatccc cggggggacg tcctgccctg
      301 ctcgtcctgg gacgaggggg tggggaacct cctgtcggat gggtttgaaa aagtctggtt
      361 cggggatcgc gcggcggccc tcaagaacaa ggagtatgcc cccgcggagt gccggacctg
      421 cccggccttc gcggcctgcc aggcggcctg ccccctgtat tggtcctacg cggggtacgg
      481 ggagatcgag gaggcgggcc cggccttccg cgcccgccat gcccgcccgg cctcgtccgc
      541 agggccggaa gcgaccgggg aaccggcccg aggaggaacc gatgagcgac ctgtccctta
      601 agccgaccgt gaccgcggga ttccggttct tccggatggg cccggcggcc cgactcatcc
      661 tgaccggggc cctcttcgcc ctgggggcgg ccctccaggt gggcgtgggc gtcctgccgg
      721 gtaccctggt gatactggcg ggctggatcc ccctgggact ccgcagggtg acgaacaagc
      781 ccaaggacca gggcctggag aaatggaagc ccgttaccgc cgcggaagtg gaccgcatcc
      841 tggacgcctt ctcccgggcc ggcaagctcc gccgggcttc cgggggccgg tccgccctcc
      901 gcatactggc gggagtcctg ctcatgatct cggtgcccgt ctccttcggg atctccgggg
      961 gagccggcct cgtggccctg gacgcccttc tgttcctctt cccggccctc ttctttggaa
     1021 aggtaaaggt cttcaccccg ggcctcatcg cccagaagct cctgggcttc gaggccatcc
     1081 gctccgaacc cctgcccgag ggctgggtcc tgacccccta catccgcttc gacgaggacg
     1141 aggaaggccg ggacgtgccc gaggacctgc gcttcctgct ggaaccgaaa aagaagatcg
     1201 gggacctggt gggcgtccag ttccagaccg ccatcaacaa cggcgccaac ggaccggtac
     1261 cctacatgta cgccgtggtc ctgacccggg gacggaaggg acccgcctac gaggcgctcc
     1321 ggggattttc cgcccggggc tacgaggtgg agcggggagg ggacgacgag tacgggaccg
     1381 tggtgatccg ccaatccacc gggggcgggg gctaccatac caagccggac cagtgccgga
     1441 agctcttccg gctggcggcc tccgcccttg cgcgggggta ggatggtgaa gtgagcggcg
     1501 aattggtggc gttcctgtcc cggtacatcc ccgtgtccgg cgacctggag cgtattttcc
     1561 gggagaacga cttcgtgcgg accttcccga agggctccgt gctcctgagg ccggaggacc
     1621 gcgcccggga ggcctacttc atcctgaaag gctgcgtgcg gagctacatc gtcaaggacg
     1681 cggaggagcg gaccgtggac ttcttcgtgg aggaggacgc ggtcctgccc ctggggtacg
     1741 gacgggagga gccggcgggc cactacctgg agtgcctgga ggataccgtc gcggtggcga
     1801 gcgacccgga ccgggagcgg gagatgctgg cccggtaccc ggagctcaag gccgtctgcc
     1861 tggccatgtc ggagatcatg gcggccaagc tccaggagaa cctggcccga taccggacga
     1921 gcacccccga ggaacggtac cgggacctgg ccgcacgccg tccggacctg ctccggcgga
     1981 tcccccagta ccggatcgcc gagtacctgg gcatccggcc tgagtccctg agccgtatcc
     2041 gccgccgcct tgcccggccc cgtcggccgg gcggcagttc cgatcgatct taacccggat
     2101 caattccctt tcccctcata tccggatact ttttgcccca tccggaaccc gcccggccgt
     2161 gtcgccgcgg aggttccggg agatcgacgg ataaaggaga atccggaatg aacgcaacga
     2221 ccgacgcacg attcctcgac gtgaaggcgg acccgaaggt ggtcttggcc ctggcgtgga
     2281 cagccctgct gggtctgtac atctattgcg acatttattc cctgttcagg cccgggatct
     2341 tggaggagat gcgggccggg aagatgggcc ccttcgatgt gagccagacc tccctggccc
     2401 tcgcctccct cctcatgatc ctgcccatcc tggcggcccc ggcctcggtc ctcctgccgg
     2461 cccgggcggg gcggatcctg aacctgctcc tggcgcccct ctatttactg gtgaatatcg
     2521 gaaacctgct gggcgaagcc tgggtctact actatctgtt cgggattctg gagttggccc
     2581 tgaccgcggg catcttcctc gtcgctctca aatggccgaa ggaacgcttc aacccgtagt
     2641 tcttcaggat aacctggcag gactcaccac ggagatcacg gagatcacag agaacatcca
     2701 agaccggaag gagtccgaca ggatggacag gatgagggac ggatcaacag gattgaatcg
     2761 agagttagat atccaaaatt cttatcttgt ccatccttct tccatcttgt tcatcctgtc
     2821 tgatctttcc gatcctttat ctctcagtga cctccgtgtc ctccgtggtt gattccggaa
     2881 aacgtcctgt gttgcggcgt gtcgggctta cagcgcctgg cccttgggga tgctggtctc
     2941 cagggggagg gccccgaaga ggagctcctc ttccggtttt tccttggcgg ccctgcggaa
     3001 tcccaggggg gagaccccgt aggctttctt gaacaggtgg tagaaatggc tgaggctctc
     3061 gaatccgacg tccgaggaga tgtccacgat cttctcgttc gtggaggcga tcgcccgggc
     3121 agcgtgctcc agccggaagt cgttgatcag ctccgtcggc gacttgcgca ggtgcttccg
     3181 gcagcttcgg gatatgtgct cctcgctctt gcccgctagg cgcttcatgg ccgggaggcc
     3241 ctcggtgaag ttctccttct tcatcatctc caggacgagc caccggagcc acaccggggt
     3301 ctcggacttc ctcttgtccg ggagaatcgg gaagcaggat acgatgatgt gcaggagggt
     3361 gatgcggaac agggagtccg agctggtcct gcggatcttc cgggagagga tcagctgttc
     3421 cagttcctgg acgatcgcct cgtgttcccg ctgggcgacc tgggccaggg ggggcaaggg
     3481 acaggacagg agccggccgg gatcgaagcc ctctcccagg tagtcgaaga gggcctgcac
     3541 ggtctcgcag gggatcagga cgttgatgat ctcgaaatcc ccggacatct cgtcgtagga
     3601 atgggagtcg tcgggccgga tgaaggcgat ggatccgggc ttgaggcgct ggaccgatcc
     3661 gttcaccagg tggtgggcgg tgccgcggtt caccaggaac agctcgaagt agtcgtggca
     3721 gtgccggatg cggagctgtc tccgttcccc gatcaggctg gcgtattcgt gggagggatc
     3781 cccggtgaag cggctcagtc tcagtttcac gagatcatta taggacaata atccggtcaa
     3841 ctcaagacaa gaccgtttcc ggtttcggcc ggaaaatgcg gcatgaacaa cttcaaggtc
     3901 aagagtccgc ggaaggacct gcggttcgag atagccttca ccaaggccta tcgggacgcg
     3961 atcgaccggt atacccatcc cgcggccatc gaggcagcct gcctgcgggc ccagttcccg
     4021 gccatcctcc acccgatcca ggacgaggac ctcctggccg ggaggatcca gatgggggtg
     4081 gtgggcttgg ggatccagca ccagaccggg ggcttcggtt tctacgccaa cgaggagaag
     4141 ctggtcgcgg agctggagtt caaggccggt gacgcccgct accgggagga cctgcacgac
     4201 cttttgacct tctggaaggg ccgcacgacc aacgccgtcg tattgcggga gatgcccgag
     4261 tccatccggg aagccctggg gagcgaccag tggcggcacc tgcccctgcc cgcctcgccg
     4321 atcctgcgga tggccggggc ctacgtggat ttcgacaagc tcgtccgcgt cgggctcccg
     4381 gggctccgca aggaggtcca ggagtataag gcccgggccg aggcgggcgg cggggacgtg
     4441 gagcttttcg cggccatggc ggacgtcctg gacctggtcg gggaggtctg cgccttctac
     4501 caggcgcagg ccctcgacct cgcggcgaag gcgggggacg ggaagcgaaa ggccgagctg
     4561 ggcctcatgt ccgccgcctt gaagagcatt caaaccgccc cgcccgcgtc cctgcgggag
     4621 gccctccagc tggcctggat ctacgggatc atggcccccc tcatcgagtt cggccgcctg
     4681 gacgagtacg ccggggattt cttcgtccgg gacctggaat ccggggtcat caccgaggaa
     4741 gaggccgtgg acctagtgtc gagcctctac cgccttatcg accacctgga ctgcgagacc
     4801 gacggacggg tgatcgtggg cggctacggc cggcgcaacc ccggaaacgc ggaccggttc
     4861 tgcctggtgg ccgtcgaggc ctgccggcgg gtgaaggagg tcttgcccca gttcaccctg
     4921 cgcttcaaca aggacacccc caagccggtc tgggaagcct cgatgcgttg catcgaggag
     4981 ggccgaacct atccgctcct ctacaacgac gacatcctgg tgcccggggt catgaaggcc
     5041 ttcggagtgg accgggcccg ggcggaaacc tacatgcccc tgggctgcgg ggagatcgag
     5101 ttcgaccatt acagcttcgg gacccccagc gggtccatga acgtcctcaa gatcctggag
     5161 ctgaccgtcc ggggcggtta cgagcccatg accggcagcc gccgggggcc cgcgaccaag
     5221 cccctggccg cctgcgcgac ctacgaggag ttcctcgcga actacaaacg tcaactggac
     5281 tattacatcg aggcccaggc ggtgttcgag aagtacgagt acgagatcac cggccggatg
     5341 caccccttca tgatggtcac cctgctgtac gacgggtgcc tggagcgggg caaggcggtc
     5401 ttcaacgggg gctgcgccta cctgggggga accctggagc tctacggcaa cgtcaacgcc
     5461 gccaattccc tggcggccat caagcgcctg gtcttcgagg agaagaagat ctccgcggag
     5521 cgcctggtgg aggcgatgga ccggaacttc gtgggccgcg aggcggagat gaagctcctc
     5581 cgggacgcgc cgaagtacgg caacgacgac gactacgtgg attccatctt cgtggacctc
     5641 cacgactacc tgtcggacag gatccgggag caggccccga aggtcggcct ggacagctac
     5701 ctggcggtca ccatcaacaa cgcccagaac accaccctgg gtcgctgggt cggggcgact
     5761 cccgacggaa ggaaggcggg tatgcccatg gccaacgcca acaacccggc ccccggcacg
     5821 gaccggaacg gggtcaccgc catgctcaac tccatcctga agccccgtca cgacaaccac
     5881 gcgggcatgg tatccaacat ccgctttacc cgggagcttt tcaccgcgtc ccgggacaag
     5941 atgcacggtc tggtgcgcgc ctacttcgac cggggcgcgg cccacgcgat gatcaccgtg
     6001 gtcggcaagg acgatttgag gaaggccatg gaacggcccg aggaataccg ggacctgatc
     6061 gtgcgggtcg ggggtttctc cgcccgtttc gtggacctca agaaggacgt gcagcaggag
     6121 atctatgacc gcgtcaccta ctgacggtcc gaccgccgtc gtctacgaca tccagcgcct
     6181 ttccctgaac gacgggccgg gcatccggac gacggtgttc ctgaagggct gtcccctgcg
     6241 ctgcgcctgg tgccacaacc cggaatcctg ggagcgggag ccccggctgg cctacacgga
     6301 atccctgtgc cgtctctgcg gggaatgcgt ccgggtttgc ccgaccgggg cccataccct
     6361 ggagggatcc gcggactccg gcttgcgtca ccggatcgat tggagcacgt gcgacgcctg
     6421 cgggaagtgc gtggaagcct gcccacacga cgccctgtcc ctatgcggcc gggagtatac
     6481 cgtgccggag ctctacgacg cgttgaggcc ggaccttccg tacttcagga tcggggaggg
     6541 cgggggcgtg accttctcgg gaggcgagcc catggtcgcc gtagatttcc tcctcgagtt
     6601 cctgaggcgg aagggaagcc tacatgtctg cctggagacc tccggacagg caccggccga
     6661 ggacttccgg aggatcgcgc cgatggtgga cctcttcctc ttcgactaca aggccacggg
     6721 ggcggaacgg catcgcgccc tctgcggaga gggcggcgag cggatcctgg agaacctccg
     6781 cctcctctgc cggtccgggg cacgggttct cttgcgcctg cccctggtgc cggggatcaa
     6841 cgaccaggag gagcacctgc gcgcggtcgc gaccctgctg gacgaactgc ccgggctcgt
     6901 gggggcccag atcctgccgt accactccct gggagccggc aaggaggccc gattcgggtt
     6961 ggaaggccgc acgctccggc tgccctcggc ctcgccggag caggggacgg cctggctcaa
     7021 gtccctggag tccttcgggg cccggaaggt tttcctgtga aatcgtgcat tcttccgctg
     7081 cggcggatag gatataaggg ggtacggaaa tgaagagaat ggggattgcg atcctggctt
     7141 tggttgccct ggccttgctg ttcgccccgg cggcggcggc ccaggacaag aaggtgaacc
     7201 ttctggtctc tcgctgggca ggaccccacg cggacttcca gaagcagatg gtgaaggagt
     7261 tccccgacgc cgccgtgcgc atcgacgaca tcgactacgg cagcctcaag cagaagcaga
     7321 tgacgtcctt ccaggccgcc aaggggaccg ggaactacga cgtggtctgg gtgaacatcc
     7381 agtggatgaa ggagtacgtg gacgcgggct accttcttcc catcgacgac cttttgaaga
     7441 agtacaaggt cgacaccaag atctacgcca aggggatgat gcagggcgcg gtcatcgacg
     7501 gcaagaccta cggtctcccg accttcgccc agtgcctgat cctggcctac gattccgcgg
     7561 ccttcgacgc ggccaagctc aaggtgcccc gcaccgccga cgacctggtg gccgtggcca
     7621 agtacttcaa ggagaccaag ggttccggca tcgccatccc cgccaagcag ggcggcgcgg
     7681 cggccaccct ctattcccag ctcctcttct cgtccggcgg attctacttc gacaagaagg
     7741 gcaagctcga cctcctgagc gagccttccg tctacgcggc gaccatctat gaccagctgg
     7801 cccggtattc cgtccgcggc gccaccgcgt ggcaccacga cgagaccgcc gaggcggtgc
     7861 gcacgaagac ggcgcccatc ggcatcatca tgagcggact ggccaaccag aaccacgatc
     7921 ccgagaagtc cctcatcgtc gacaccgtca agtacgcgac cctgaacggg aagaccgggg
     7981 acaccgcggc caacaacgcc tactgggtct gggccatcgc caagaacacc aacgacgtgg
     8041 acgcctcctt caagttcatc tcctggatga ccagccccgc gatcgagaag aagcagaccc
     8101 tggcgaacaa gcagatcagt gccatcacct ccctgtccga ggatcccgag gtgcagaagg
     8161 ccatgcccta cctctcggtg gtcatgaagg aactggcgaa cgggaagatg gaccccgccc
     8221 tcaagaactt ccagaccctc aagaacgccc tgatcgtggg tctctcggag atcgcgacca
     8281 cggacgcgaa gcccgaggac gtgctcaaga agatccaggc cgacctggcc gccacggact
     8341 tcagcaagtg accctgcgcc gtccggtccg ttctcgggcc ggacggcgac tttcttgacg
     8401 cggccgcgca cccggagctc cgggtgcggc cgtgatctcg aaccggacca cggaggaagc
     8461 atgcggaagc cccgcgccct gtccctcgag aaaggcgacc ttttgcccac cctgctcatg
     8521 gcgcccgccg tgagcctggt cctgggcgtg atggtcgtac ccctggcgtt cggcgccttc
     8581 ctcagtttct ttgacttctc cttcgggtcc ttcgaccccc ggaccgcctt cgtgggcctc
     8641 aagcactacc tccggttctt ccgggacggg acggccctga aggctttgtt gaacaccctg
     8701 atgttctccg ccggggcgat cgccggggac ttcacgatcg ggaccctggc ggctgtcctg
     8761 atccattccc tctcccgccg cctggcccgc ttcgtccgcc cgatcgtgac ggtgcccctc
     8821 ctggtgtcgc cgattgtggt gggcctcatc tggcgctaca tctacgatcc ccagggcatc
     8881 ctgtactgga tgatggaccg ggtcggcatc cccttcgagt tcttcccggg ggtgaccagc
     8941 gcctccacgg ccctgctgtc cacgatcatc gcccactggt ggcaggtggt acccttcctg
     9001 atcatcgtcc tgaccgcggg cctggtatcc attccgacgg agctctacga ggccgcacgg
     9061 gtggacgggg cgggggcgct gtccatcttc ttccggatca cggtgcccct cctgcgggac
     9121 gtatacctgg tgatcctgtt gatcagcggg gtggatacca tcaaggtctt cgacatcatc
     9181 ttcgccctga ccgggggagg cccgaacaac tcgacggtct ccctgagcat ctacgcctac
     9241 acccagggct tcgagaacgg ggacctaagc tacgggatcg cggtctccat ggtcgccatg
     9301 atcgccacct tcgggatctt cgggatcccc ttcatcctgc agaaccggaa gcggaggggg
     9361 agctgatgac acggaatacc cgcatgattg ccccgagcaa gtggccgccc cgcctggcca
     9421 aggccttctt cctcctggtc ttcgggctct ggaccgcggc gccggtctac atcatcgtgt
     9481 ccaacgcctt ccggcggacc ctggacataa agaagatgcc tccggacctg atcttcaagc
     9541 ccatcttcac ccacttccag cgcctcttcg agctggacga cttcctgcgg tacttcgtca
     9601 actccatcgt catatccgtg tccgtgaccg tcacgaccat agccctgggc acccttgctg
     9661 cctacgggct caagctcttc aagtcgaggc tcggggagcg gctttccaac ctccttctga
     9721 tcgggaagct cgtgcccgcg atcacggtgc tcctgcccat gttcatcctg atgaaccgtc
     9781 tgcgcctgac agggacctac ctgggcccca tcctggcaca ctccgccatg ggcctgccct
     9841 tcgtcacctg gatcatggcc tccttcgtgc gggacatccc cggggagatc ctggattccg
     9901 ccaccgtgga gggctgttcc cggatgaagg ccttctcccg catcatcttc cccatgctca
     9961 ccccggcgat cgcatccgcg gtgatcctga tcatgcagtt ctcctggaac gagcttctgt
    10021 tctcgctcca gctgaccaac ctggacacct atcccctgac cgtcgggatc gcccgctacg
    10081 tgggggccat ctcggtggac tggggcaagt gctccgccgc cgccaccatc agcatcgccc
    10141 ccatcatcgt ggtcggcttc ctgatgcaga aatacctggt caccggaatg accgccgggg
    10201 ccgtcaaggg ctgatccgcc ctgcgggttt gggagacatc catgattttc ggcgcgcagt
    10261 actacaggcc tccgttcccg ggccgggaat gctgggaccg ggacctggcg aacatggcgg
    10321 acctgggctt caacaccgtc aagctctggg ccgtgtggaa ctggatcgag ccggtccggg
    10381 gccgcttcga tttctcggac ctggacgaac tctgcgggct cgcggagcgc cggggcctgg
    10441 cggttgtgat caacacgatc cccgagggct cgcccccctg ggtcctgccg ggcaacgagg
    10501 acgccctgta ccgtaccggc cggggtgagg tcatcccctt cggcgggccg ccgaacctcc
    10561 cctccgcggg ctggcccggg ctctgcatgg acaagccggc ggccgcggac ctcacggccc
    10621 gcttcatcga agccgtggcc gcccggtacc gaacggcccg ttccgtcgtc gccctggacg
    10681 tgtggaacga gccccacctg gagcccatgt tcgactaccg gggggagctg ttgtgctact
    10741 gcgagcactc ccgggccgcc ttccgccgct ggctcaagga caagtacggc gacctagggt
    10801 ccctgaacgc cgcctggttc cgccgcctct cggactggtc cgaggtcgag ccgcccccgc
    10861 gcttcggaac ctgggcggac atgctggact ggcggctctt ctggatcgaa aacctggccc
    10921 ggcacctgcg gatccggacg gaggcggccc ggcggggcgc cccgggcacg gcgatccaga
    10981 cccatgtggc ctacagcgcg gtcctgggca acaagctggc cgggggtctg gccaacgaac
    11041 tgggggacga gttctccctg gcccgggagg tggacgtctt cggcctctcg agctttccca
    11101 agtggctcca gggcaaaaac catcccttca cccacctggc ccacaacgag gtcgtggccg
    11161 aggccgcccg gggcaaacgg tattaccagg tcgagctcca gggcggggga gggaaggccg
    11221 gcttcctggg gggcgaggtt ccgacggccc gggacgtgcg gatctggaac tacaacaccg
    11281 ccgccgcggg cgggaagggc gtcctctact ggcagtacgc cccggagccc gcgggcctgg
    11341 aatccccggg cttcggactc accgcgtacg acggggggaa caccgagcgg tcccgggagg
    11401 cgggggcctg cgcccgggag ctctcggatc cccgcctgga cgcggcgacc cgggtcctcc
    11461 cggtcaacgc gatctacctg tcccggaaaa gccaggttct ctccttctgc gccgagcgcc
    11521 gggaggacct ctacgccggg agcgtggccg ggatctaccg ggccgcctac gaggcgggga
    11581 tccccgtccg cttcgtccac gaggactacc tggaaagcct gggcgtcgag ggggtccgga
    11641 ccctcttcgt ccccatggcc ctctgcctgg acgaccggga gcgggccgcc ctccgggcct
    11701 tcgccgaggc cggcgggctt ttggtcggag aggccgccac ggggatgtac gactcaggcg
    11761 gggcattgga cccggacagc cgggccctgg ccgacctctt cggggtgcgg cacgtcgagc
    11821 tcgaggccct gcccgattgg ggcttcaccg aggctctcgg ggacggcttc tccttcggag
    11881 gcgcgcaata ccgtcgcgtc gtggaacccg ggccggggac cgaagtcctt gcccgcttcc
    11941 cggacgggcg tcccgccgcg accggccggg ccctgggggc cggccgggcc gtctatgtag
    12001 cgaccttcgc ggccctggct ttccacctcc ggggggaccc ggggacgcgg aacttcctgg
    12061 cgggcttcct cgatcccgcc gggtatcccc agctccgccg cctggccgtc ctccccgggg
    12121 aggagccgga acgggccgag ggcgccgcgg cggcgccgtc ttcggctgcg acgcgtgcgg
    12181 cggccgtcag cgcctcgtcg gctccggcgg ccgcgccggg cgcaccggcc gcgccggccg
    12241 ccttcgtccc gagcgcggtc gtccgcctcc tggaaacccc ggaggactgg gtcctggtgg
    12301 cggtgaacca ccgggagggc cccgcccgga tcgaactgga cttcgactcc gccctcgtcc
    12361 gggaggcctc cgcgtccctg accgtcccgg gagggtccgg gaccgtgcgg tatttccgga
    12421 aggccgggcg gacctagcgt acatagcggg ctcacggcga aggccgcccg cgcttgcccg
    12481 gccgttccgg ccgcgctaca ctgtcggcct cgacccgaag gaggcccccg atgaagaaat
    12541 tcaaggacat cccctacgca cggcccgaca tcgaggccga gtccgcggcc ttccgctccc
    12601 tgctcgcgga cttttccggg gcccggagcg ccgaagccgc ccgggaggcc atggaccgcc
    12661 tgtccgccct ccgggaccgg atctccaccg tgaaggacct ggccgagatc cggcacacct
    12721 gcgacacgac ggatcctttc tacgaggcgg agaacgattt ctgggacggg gcgtctcccc
    12781 ggatcgacgc cctggagcac gagctctacg cggccctggt ctcctcgccg ttccgaaaag
    12841 agctggaggc ggtctggggc ccccacatct tccggatggc ggagtgcagg ctcgctgcct
    12901 tcaagccggg gatcgaggag ctcctggcgg cggagaaccg cctgtccagc gagtacgaca
    12961 agctcaaggc ctccgcttcc atacccttcg acggacgggt cctcaatctg gccgagctcg
    13021 gaccctacat ggaatccccg gaccgggaga cccgaggccg ggccgtgtcc gcggtctccg
    13081 ggttctaccg ggaccatggt gacgagttcg accggatcta cgccgaactc gtggcggtcc
    13141 gtcaccgcat cgcgctcgag ctgggcttcc cgaacttcgt ccccctggcc tacgcccgcc
    13201 tgggccggac ggactacggc cccgccgagg tggcctccta ccggtcccag gtgctccgga
    13261 ccgtggtgcc cctggccgcc tccctccggg agcggcaggc ccgacgcctg ggacttccgg
    13321 tcctggccta ccgggacgag gccttcgagt tccccacggg caatcccagg ccggtcgggg
    13381 gcaccccggt cctcctggaa gccgcgggga agatgtacga cgagatgtcc gccgagacgg
    13441 ggtccttctt ccggatgatg cgggagcggg agcttttcga cctggagacc cggaagggca
    13501 aggccctggg aggctattgc tcgtatctcc ccgccgaggg cgtccccttc gtcttcgcca
    13561 acaacaacgg gacctccgac gacatcgaca ccctgaccca cgaggtcggc cacgccttcc
    13621 aggtctcccg gggcgggcgc ttcgcgttcc cggagtacca ctggcccacc ctggaggcct
    13681 gcgagatcca ctccatgtcc atggagttcc tggcctggcc ctggatggag ggattcttcg
    13741 gcgcggacgc ggacaagtac cttttccggc acctgaccgc ggggatcctc ttcctgccct
    13801 acggagtcct cgtggacgag ttccagcact gggtctacga gcatcccggg gcggcccccg
    13861 ccgagcgccg ggccgcctgg cggaggatgg agagagtcta ccttccgtgg cgggactacg
    13921 agggcgacgg attcctggag gccgggggct actggttccg ccagggccac atcttccagg
    13981 accccttcta ctacatcgac tacaccctgg cccaggtctg cgcctacgag ttctgggccc
    14041 tgtcccggtc ggaccgcccc ggggcctggc gccggtacct ggccctctgc gacgccgcgg
    14101 gtagccggcc cttcctggat ctcctcgggg tcgcgagcct gcggaatccc ttcgcggagg
    14161 gctccatcgc cgggatcctg gccccggccc ggacctggat ggacgccgtg gacgaccggg
    14221 ggttctaggg aggggatgca tgtcctcata gaatcaagca cgcatacggg tttccgcggc
    14281 ctgtcggggc gggagcgcat gcataacccg tgccgcccgc cgggcgtcgg ggcattgcgc
    14341 tgagcccgta ccgggcttag aatttcttcc gatgggcggc cgaggagcgc catgcttcgc
    14401 ggccgcccga aggaaaaacc atggtggagc gcgtacgcga gagcgtccgc ggatccggtc
    14461 cggtccggaa tccgcggctc ccggcatcgg cgtcccggag tctgccgcgg ggggtcctgc
    14521 tcgcgctggt cctcctggcg ttcgcggacc ccgggggagc ccaggtgacc ggactgtccg
    14581 gcatccggtt cgaatcgccc atccccgtcc ccgtctatct cctcgaccga acgatcgcga
    14641 tacccgaagc ggtgcccggg tattccgagc ccgactatat attccgggcg gaaggaggga
    14701 accggaccct tcgcgccctg gacctgtacc gctacctggg ggactccccg gtcgccttgg
    14761 atcttacccc cggggcatac acgttccagc tggttctgcc cgggaacgaa gtccgaacca
    14821 tccaggtgga cacgagggaa cccggggacc ggacccagac ttgggagatc cgtccgggcc
    14881 gggagggtgc gaacgttctc ggcctgactc tgtccctggc cgggggcatg gtctggtggg
    14941 gcgccgtgag tctcgccgtt cgggaatggt tcgtcgagga cgagttcggc gcggcctcca
    15001 tcgtctgggc ttcgagccat gcgctattca tcggaggact ggttatcgcg ttcggccccg
    15061 gctctccgag gatacgccgg atcgaatagc ggggcctccc cagccgttcc tcgggcgcgg
    15121 gcggccaaga agcctaaggt tttcggaggt gtgccaggat gcgctttcga aagctgtgtt
    15181 gttcgtgcat cctgatcctg ctgtccgcgg catccgccac agcccagatc gatcctctcg
    15241 tggaggccgt ccgccggggt tccctggacg acatccggaa gatccttgag tccggagccg
    15301 acgtcaacga aaggaacgcc ggcgggatga ccgccctcat ggccgtggcc cgggacgggg
    15361 acg
//
LOCUS       DAOTEI010000074        15286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2654_length_15286_cov_5.702974, whole genome shotgun sequence.
ACCESSION   DAOTEI010000074 DAOTEI010000000
VERSION     DAOTEI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2654_length_15286_cov_5.702974"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="P5313_08670"
     CDS             <1..586
                     /locus_tag="P5313_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRY80475.1"
                     /translation="IEYEFPMGWKELEGVHNRSDYDLTRHQQFSGKDLQYIDQDNGNA
                     RYIPFIIETSAGLTRQLLMVLCDAYEDEKVADKGNEDDWRTVLRFHPALAPVTVAVLP
                     LMKKDGLAELAQDIRKDLKEDFATDYDQGGAIGRRYRRQDEIGTPFCVTVDYQSKEDG
                     TVTVRYRDSMEQIRVPRAELAARIRADIKAYKRV"
     gene            619..1968
                     /locus_tag="P5313_08675"
     CDS             619..1968
                     /locus_tag="P5313_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRY80476.1"
                     /translation="MAAILGLGFLTGRGVRDASDFDTGGNRAGPVLTAGAIVGTLVGG
                     SSTVGTAQLAFLHGLSAWWFTLGAAAGCLFLAALCGPLRSARSGTIQEIIRREYGAVA
                     EVVTSVLASAGFLINIAAQILAADALLETLFGLPPAATAALGVLLMAGYGVFGGIRGS
                     GILGIFKMALVYLAAAVAGLLALRLSGGIGTFLRELPRDGYFNLFTRGIGTDLGAGLS
                     VALGVLSTQTYVQAVLAARSLRAARGGALLSAAIIPPVGILGILVGYHMRLRFPDLPS
                     GQAFPRFLAENLPPAWAGVFLAALLIAVLGTGSGMALGFGRIVVNDIYLRIRPGADGK
                     RTLRVSRTVILAALAGSALLILGVPGSTILSFGFLSMELRAAVLLIPMLAALFLPGRM
                     RGGWAIASSAAGLAAVLASKAAGLPFDPLLPGIAAGAVVAGAGLIAGGPRSGTTGTG"
     gene            complement(1984..2466)
                     /locus_tag="P5313_08680"
     CDS             complement(1984..2466)
                     /locus_tag="P5313_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HRY80477.1"
                     /translation="MTETCPCGSGREYSACCGPVIAGAVPAPTAEALMRSRYTAYVKG
                     EIDHILGTYARADGANRKATEDWSRKAVWKGLEIHRVEKGGAGDTEGMVEFSAKFETD
                     GRAEEHRETAYFIKKAGTWLYDSGNVRTETVVRAGPKVGRNDPCPCGSGKKYKHCCGK
                     "
     gene            complement(2524..3216)
                     /locus_tag="P5313_08685"
     CDS             complement(2524..3216)
                     /locus_tag="P5313_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY80478.1"
                     /translation="MNGTKFLALPFLSVGCLAAAAAQGAVQTELTYFGRATVKIRTVS
                     GFVLYIDPYAPGDYSEPADLVLVSHGHGDHNRVDLVKLKPGAAIASAPGAVPGKGVRF
                     LAEGDSFQAGPVTVRALPASNKNHRRGETLGFLVSFDGIVVYHASDTNWLSEMEGFAK
                     YGIGYALLPCDGFYNMGPEEASRCAAAVKARVVIPIHSSKSELFDGKNARAVRGPEVL
                     VLEPGQSTALKP"
     gene            complement(3226..4878)
                     /locus_tag="P5313_08690"
     CDS             complement(3226..4878)
                     /locus_tag="P5313_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRY80479.1"
                     /translation="MTITEVESVVLSVTVYQGRAMITREAKARLAAGDHVLVFPGLPS
                     DLDRDSLQVKGTGEAVLGECVFETEYFEEDVDGKRRPLLAERRRVSDEAEELNLHLSR
                     LDGEKAFLDRISSLLTTPPPGGTPQGGPSAGPGTPCPQTVLDVGTWSGITDFYRSKHS
                     ELDSLRLTAERKLRDISDKIENLDARLEDLGASGRRSREVVKVGIRKKTAGDLVLRLS
                     YMLSGPSWRPVYNLRASGDSDTLQLEYDAYISQATGEDWSGVELRLSTARVNVSGVIP
                     ELRPWRLEFHRPRHAVLRSVMAKESAKADLREVADFASGAAPSAPYEEEDAAELDFER
                     DEAEVRDSGASVVFTVAGGGNVSGDNKDTRVGVARREFPAAFLHKAVPKLAEFAYLTA
                     RFKNEADFPILPGAVNIFFDGSFVSGSAFGLIMPGQETEVSLGVDEGVKVEYRFLKRF
                     RKGEGLVNKRVSEQFEHQIRVTNNRTRPVDLSVSDQFPLTSDKDLVVKALQPQARENP
                     REVALDDESRITWTFSLKPGEKRELPLAYLVEYPSDRRVEGL"
     gene            complement(4998..5402)
                     /locus_tag="P5313_08695"
     CDS             complement(4998..5402)
                     /locus_tag="P5313_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80480.1"
                     /translation="MKRLRSLPLAGVIVVAFALTLALPAAAQSAYDRDVVVQVMRTNL
                     QTLGQVRAALNKGDFYAAAEGFWKFAEGQNRILQFTPPKGSKAEWDRILGKFVDTALR
                     GVGAAGERDAPKGLKIVEELQALNKEGHSLFR"
     gene            5575..6273
                     /locus_tag="P5313_08700"
     CDS             5575..6273
                     /locus_tag="P5313_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRY80481.1"
                     /translation="MSDVNPDSERIVLEHLRDQIVSGKLKASTKLPSERALCQTLGVS
                     RGYVRKALAKLEHYGLIRTLPQRGTIVAGLGSKAISGLIASIGSLDEDFGPVELFEIR
                     ALLETFAARKAAERAGADDITEILKWHSEFRIRADAGQRGLEEDHLFHLAIARASGNP
                     VCLALTSYFTPQIISLNADYAESDPDRFRRTFAEHDAIVRAILAGDPDVSAEAMRSHM
                     EEAWKRRLPERSPR"
     gene            6355..7581
                     /locus_tag="P5313_08705"
     CDS             6355..7581
                     /locus_tag="P5313_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY80482.1"
                     /translation="MKKVFALAVVLSMIALAVAAAPKPVTITHWYWADNPKYSAVMKE
                     MAADFNATNGKGITVVVQEYPWDGGGYSETLFRAAMGGGGPDTSSAKLTSTPLFVSNN
                     LWENLDKYINRWKDKDQIDPALWKTMRDASRTKNVFLMPWTTQVLYVYYRPSMFKAAG
                     ISVPKNYAEFLDACAKLTKDTDGDGRTDVYGFGMRGFRGGHEPWGSFIQARGGTFADF
                     TTAKAVAGMQDFIDLFKKGYVPPTAPMDGFNEIIANFKSGKTAMTVHHIGSSTGMIDT
                     FGDDVDAFPFPGAEGKWTSMGDTENVMFRSSKNKDAAFEWLAYLAAGKGQITWCAATG
                     NVPVSKAVQKLPQFQSSRFMKVSIEGAPFAGIYPVLDTTTEWISTIWPNTIAAALQGK
                     ISAAEAMKQLQTGLYK"
     gene            7725..8606
                     /locus_tag="P5313_08710"
     CDS             7725..8606
                     /locus_tag="P5313_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY80483.1"
                     /translation="MGRKRNPWPYLLVAPALLTVLTVVFIPVVNAVSMSFQSYDLRRP
                     SRIGFIGLGNYAEVLSDPLFWQSVWKTVVWVGVGVGFQFLFGFILALLLNRRFFGRGI
                     VRSISLIPWVTPGVLIGLMWRWIYDGSYGVLNDILLRLGIIRDYVPFLARGDTAFPAV
                     ILTIIWQGIPFFALMILAGLQGIPEELYDAADVDGANGVQKLFRITIPSLRNVIFVTT
                     LLRIIWVSNSVDVIFNLTEGGPAYATQTLSVYVFNKGNSLNLGYAATMSILLALMLSS
                     VAVVYLRRIFRNQEAAR"
     gene            8603..9439
                     /locus_tag="P5313_08715"
     CDS             8603..9439
                     /locus_tag="P5313_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY80484.1"
                     /translation="MKRSPAGAAKNLVSYLLLAGMLVFLLFPFYWTFVTSIKPESELY
                     GKTVTYWPRKATFESYTRLFSDYNFIRPMTNSLIVAASTTLVSLTVSLLAAYSFSRFQ
                     FKGRKFLMTLFLTNNMFPTVLLLIPLYAIMRKLGILYTPFALILAYTTFTIPFSIWLL
                     NGYLADLPPSLEEAAMVDGANRAQAFVRIVLPVLMPCIVATGAYVFMTSWNEYTFAVM
                     FTNEANRTIPVSLKNLIGQLGVEWGMLTAGGIITIVPVCFMFFFAQKRLIEGLTAGAV
                     KG"
     gene            9459..10277
                     /locus_tag="P5313_08720"
     CDS             9459..10277
                     /locus_tag="P5313_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY80485.1"
                     /translation="MAHEPQDATARRIRRDILKMLYLCQSGHPGGSLSCVEILEGLYY
                     RTMRVDPSRPGWEDRDRLVLSKGHACPALYAVLADLGFFPREDLWRLRQVDSHLQGHP
                     DMTKTPGVDANTGSLGQGLSIAVGLALAAKHKKKDYRVFAVVGDGEVQEGMIWEAAMA
                     AAHYKLDNLIVLLDHNGLQIDGPNDKVMGLGDIVGKFSAFGFDCEVVDGHDAQAVAEA
                     AGRRADGKPRFIRCRTVKGRGVLFMEGNAGWHGKPMNAQEYEAAMKELGGADHV"
     gene            10270..11202
                     /locus_tag="P5313_08725"
     CDS             10270..11202
                     /locus_tag="P5313_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY80486.1"
                     /translation="MSDAKSLRVAYGEALVELGAQDDRVVVLDADLAHATMTSLFEAK
                     FPDRFYNFGLAEANMMCAAAGFAHSGLLPFASTFALFGTGRAYEQIRNSIAYVNANVK
                     FGLSHSGLSVGEDGGSHQSIEDIALMRVVPNMTIFVPCDPIETRKAVFAAAKIEGPVY
                     IRVARPVVETITTPQTPFVPGKIVRLRDGTDVCILATGLMVKEALKAADLLASEGIRA
                     AVADVHTLKPFDAEFVLEAARTCGRILTVEEHSVIGGLGSASAEVLAGASAAKFRRMG
                     IGDKFGFSGKPADLFESFGLTAGDIARECKALLD"
     gene            11218..11628
                     /locus_tag="P5313_08730"
     CDS             11218..11628
                     /locus_tag="P5313_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HRY80487.1"
                     /translation="MNIESRGHEGEGLARVYENDRWMVAVKNWKPANDLEGIDCLERH
                     NETDELFVLLAGRCVLLSGTEKPGGGLDIRAHSMEPGKLYTIPRGLLHNTVTRRDTKL
                     LLVEAPDTSMANSEVVSLDAEQKASVRALARPGL"
     gene            11636..12577
                     /locus_tag="P5313_08735"
     CDS             11636..12577
                     /locus_tag="P5313_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRY80488.1"
                     /translation="MRISEVVAPRRSRVSEATVPEPGANDILVRVRACGVCGSEFPTW
                     TEGRDPPVRLGHEVAGEVAAAGTGVSGFEPGDRITGLFRQGFAEYAVCPAEAAAPIPG
                     GMGYEEASLGEPVACAVSGALRTDVGLGKTVAVIGLGFMGLLTLQLLRMKGAFRILAI
                     DARPETGAAARRLGADEFFLPGEVPPGLLLTDSAGAGGVDVVAECSGSGAALELAVRM
                     LKRHSVLSVVGYHQGGPRSVDFQMLNWKAADVINAHEKRTDFKMECLRIGLDLAAAGR
                     LELGSLITHRYPLEEADRAFADYESKPDGYIKGLVLP"
     gene            12596..13711
                     /locus_tag="P5313_08740"
     CDS             12596..13711
                     /locus_tag="P5313_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY80489.1"
                     /translation="MRRIRAGIVGVGKIGLVHLEALRRLGYAEAAAVVVRDEARAKAL
                     CEFYGIPKYYLDYRDLLADPGIEVVHDCTPNREHFRINGDALRAGKPVLSEKPLTVDS
                     RESAELVEVAEATGLRTGVNFVYRHYAVVQHLRGMIEEGRLGVVRAVHGEYLQDWLLK
                     ETDFDWRVRSEIGGPSRALADIGSHWLDLAIFLTGCPIREISADTATFLPFRVEPDPS
                     GKGEGRRVPVDTEDYGAALLRFEGGVRGVFAVSQVSPGRKIGLSIQVDGSLASARWCQ
                     DSPDRLWIGRRDGPNEDFVLHPSLLNRRGKSVHSYTGGPERWPDAQKNMIDSFYRTIL
                     DGVEPLYADFKAGHSVVRVVESALESGRTGCRVTVAG"
     gene            complement(13733..14092)
                     /locus_tag="P5313_08745"
     CDS             complement(13733..14092)
                     /locus_tag="P5313_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY80490.1"
                     /translation="MRIIIAEDERLIAMGLAAVLRELGHEVQAICSSGVSCVQKEREL
                     RPDLVFMDICMETRMSGIEAARKIRETGGAPVIFTTAYDDSDTAAAAAGTEPAAFLVK
                     PLMPKDIEDALRSLRAS"
     gene            complement(14089..>15286)
                     /locus_tag="P5313_08750"
     CDS             complement(14089..>15286)
                     /locus_tag="P5313_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY80491.1"
                     /translation="GSENNALSFLLADLLEEILSRSDNPGACSTHLAGRIREIVGVKT
                     VLVFQCSEFSDSHAHELISAVPDRRRTTAESREILTLLEAAHGFAASRFVDPGEDAGD
                     PRDAAVRDALRGAGFGPSILVPLRYGGGQVGALLLLDILDRHSMGNLLVSLDRLSGVL
                     ALVLRNSFMYLKLEDTVRRRTEDLLAEREQLRSALEEKNVLLQEIHHRVKNNLQIIES
                     LLYLQGQQNAGSPAAPALLTARGRISSMALVHEELYSADDLSSVDLERYIPRLTRSIL
                     ESHGCGIVPTFSMGTLRVSLSAGIHIGLILNELITNSVKYAFRGSTTGRMDIRVDSDE
                     SNCYIALTDDGPGLSTDSERTDRESLGMSIVRSLCEQFYGDVRWTNGQPGLRVEVRLS
                     REALGR"
BASE COUNT         2639 a         4906 c         5044 g         2697 t
ORIGIN      
        1 catcgagtac gagttcccca tgggctggaa ggagctcgag ggcgtgcaca accgctcgga
       61 ctacgacctg acccggcacc agcaattctc cggcaaggac ctccagtaca tcgaccagga
      121 caacggcaac gcccggtaca tccccttcat catcgagacc tccgcgggcc tcactcgcca
      181 gctcctcatg gtcctctgcg acgcctacga ggacgagaaa gtggcggaca agggcaacga
      241 ggatgattgg cggaccgtcc tgcgcttcca cccggcgttg gccccggtca cggtcgcggt
      301 cctgcccctc atgaagaagg acggactcgc ggagctggcc caggacatcc ggaaggatct
      361 caaggaggac ttcgccacgg actacgacca gggcggggcc atcggccgcc gctaccggcg
      421 ccaggacgag atcggcaccc ccttctgcgt gaccgtggat taccagtcca aggaggacgg
      481 caccgtcacc gttcggtacc gggattccat ggagcagatc cgggttcccc gggccgagct
      541 ggccgcccgg atccgggcgg acatcaaggc gtacaagcgg gtctgatcct gccgctcctc
      601 gtctccgcgg ccctgacctt ggcggccatc ctgggcctgg gtttcctgac cggccgcggg
      661 gtccgggacg cctcggactt cgacaccggg gggaaccggg ccggcccggt cctcacggcc
      721 ggcgcgatcg tcgggacctt ggtgggaggc tcgtccaccg tcgggaccgc ccagctcgcc
      781 ttcctgcacg ggctctccgc ctggtggttc accctgggcg cggccgcggg atgcctgttc
      841 ctagcggccc tctgcggacc cctacggtcc gcccggagcg ggaccattca ggaaatcatc
      901 cgcagggaat acggagccgt ggcggaagtg gtcacctccg tcctggcctc cgcgggattc
      961 ctgatcaaca tcgccgccca gatcctggcc gccgacgcac tcctggagac ccttttcgga
     1021 cttcctcccg ccgcgaccgc ggccctcggc gtcctcctga tggcggggta cggcgtcttc
     1081 ggggggatcc ggggatccgg gatcctgggc atcttcaaga tggccctggt ctacctggcc
     1141 gcggcggtcg caggcctgct cgccctcagg ctctccggcg gaatcgggac tttcctgcgg
     1201 gaactaccgc gggacgggta tttcaacctg ttcacccggg gaatcgggac ggacctgggg
     1261 gcggggctgt ccgtggccct gggggtcctc tcgacccaga cctacgtcca ggccgtcctc
     1321 gcggcccgga gccttcgcgc cgcccgcggg ggtgccctcc tctccgcggc tatcattccc
     1381 cctgtgggaa tcctgggcat cctcgtggga taccatatgc gactccggtt tccggacctg
     1441 ccgtcggggc aggccttccc gcggttcctg gcggaaaacc ttccgcccgc ctgggccggg
     1501 gtcttcctgg ccgccctgct catagccgtc ctgggcaccg gttccgggat ggccctgggg
     1561 ttcggacgga tcgtggtgaa cgacatctac cttaggatcc gtcccggggc ggacgggaag
     1621 cgaacgctcc gggtgagccg tacggtgatc ctcgcggccc tggcgggatc ggccctcctg
     1681 atcctgggcg tgccggggag caccatcctg tccttcgggt ttctgtccat ggaactccgg
     1741 gcagccgtac tcctgatccc catgctggcc gccctcttcc tgcccgggcg gatgcggggc
     1801 ggctgggcga tagcttccag cgccgcggga ctcgcggccg tgctggcctc gaaggccgcg
     1861 ggccttccct tcgatcccct cctgccgggg atcgcggcgg gggccgtggt cgcgggcgcg
     1921 ggcctgatcg cgggtgggcc tagatccgga acgacgggga cggggtaggg gaggccgtcg
     1981 gcctcacttc ccgcagcagt gcttgtactt tttcccggac ccgcaggggc agggatcgtt
     2041 gcggcctact ttcggtccgg cgcggaccac ggtttcggtc cggacgttcc cggagtcgta
     2101 gagccaggta cccgccttct tgatgaagta cgcggtctcc cggtgctctt cggcacgccc
     2161 gtccgtctcg aacttcgcgg agaactcgac catgccctcc gtgtcgcccg cgcctccctt
     2221 ctcgacccgg tggatctcga gacctttcca cacggccttg cggctccaat cctctgtggc
     2281 cttccggttg gcgccgtccg cccgggcgta ggtgcccagg atgtggtcga tctctccttt
     2341 gacgtaggcg gtatagcggg agcgcatcaa cgcttccgcg gtcggggccg gaacggcgcc
     2401 ggctatgacg ggtccgcagc acgcggagta ttcgcggccc gagccgcagg ggcaggtttc
     2461 ggtcatggtg gatcctccgg tgggaggttg tataccggat cggcccgccc gggcaagcgc
     2521 atgctagggc ttgagggccg tactttgtcc tggttcgagg acgagcacct cggggccccg
     2581 gacggcccgg gcgttctttc cgtcgaacag ctccgacttg ctcgagtgga tcgggatgac
     2641 gacccgggcc ttcacggcgg ccgcgcatct tgatgcctcc tcggggccca tgttgtagaa
     2701 cccgtcgcag ggcaggaggg cgtagccgat cccgtacttc gcgaaaccct ccatctcgga
     2761 cagccagttc gtgtcgctgg cgtggtacac gacgatcccg tcgaaggaga cgaggaagcc
     2821 gagggtctct ccccgccggt ggttcttgtt ggaggcgggg agtgcgcgga cggtcaccgg
     2881 gccggcctgg aaggaatccc cttccgccag gaaccgcacc cccttcccgg ggacggcgcc
     2941 cggagccgat gcgatcgccg cgccgggttt caacttgacc aggtccaccc ggttgtggtc
     3001 cccgtggccg tgggaaacca ggaccaggtc ggccggctcg gagtagtccc ccggtgcgta
     3061 tggatcgatg tacaggacga atccggatac cgtccggatc ttgacggtcg cccggccgaa
     3121 gtaggtaagc tcggtctgaa ccgcaccttg tgccgcggcg gcggccagac agccgacgga
     3181 gagaaagggc agggccagga acttcgtgcc gttcatggac gcctcctaaa gaccctcgac
     3241 cctgcggtcc gaggggtatt cgaccaggta tgccagaggc agttcccgct tttctccggg
     3301 cttcagggag aaagtccagg tgatgcggga ttcgtcgtcc agggcgactt cccggggatt
     3361 ttcccgcgcc tggggctgga gagccttgac gaccaggtcc ttgtccgagg tcaggggaaa
     3421 ttgatccgaa accgaaaggt ccaccggccg ggtccggttg ttggtgacgc ggatctggtg
     3481 ttcgaattgc tcgctcaccc gcttgttgac caacccctcg cccttccgga accgtttgag
     3541 gaatcggtat tccaccttca ctccctcgtc gacgcccagg gagacttccg tctcctgacc
     3601 cggcatgatc aggccgaagg cggagccgga cacgaaggat ccgtcgaaga agatgtttac
     3661 tgccccgggc aggatcggga agtccgcctc gttcttgaac cgggcggtga ggtaggcgaa
     3721 ctccgcgagc ttcgggacgg ccttgtgcag gaaagccgcg gggaactccc gccgggccac
     3781 cccgacccgg gtgtccttgt tgtccccgga tacgtttccg cccccggcca cggtgaatac
     3841 gacgctggcc ccggaatccc ggacctccgc ctcgtcccgc tcgaagtcca actccgccgc
     3901 atcttcctcc tcgtaggggg cggacggagc ggctcccgag gcgaagtcgg cgacttcccg
     3961 caggtccgcc ttggccgatt ccttggccat gacggagcgg aggaccgcgt gacgggggcg
     4021 atggaattcc aggcgccagg gacgcagttc cggaatcacc ccggacacgt tgacccgggc
     4081 ggtggacagc cggagctcga cgccggacca gtcctccccg gtggcctggg agatgtaggc
     4141 gtcgtattcc agctggaggg tgtccgagtc tccggaagcc ctaagattgt acaccgggcg
     4201 ccaggaaggt cccgagagca tgtaggaaag ccgaaggacc aggtccccgg cggttttctt
     4261 ccggatcccg accttgacga cttcccggga tcgacggccg gatgctccca ggtcttccag
     4321 ccgcgcgtcc agattttcta tcttgtcgga gatatcccgc agcttccgtt ccgccgtaag
     4381 cctgagggaa tcgagttcag aatgcttgga acggtagaaa tcggtaatcc ccgaccaggt
     4441 cccgacgtcc aggacggttt gcgggcacgg agtcccggga cccgccgaag gtccgccctg
     4501 gggtgtcccg ccgggagggg gagtggtcag aagggaggat atcctgtcca ggaaagcctt
     4561 ctcgccgtcc aggcgcgaca ggtgcaggtt gagctcttcg gcttcgtcgg acacccggcg
     4621 acgttccgcc aggaggggac gccgcttccc gtccacgtcc tcctcgaagt actccgtctc
     4681 gaagacgcat tcccccagta cggcttcccc ggtgcccttg acctgaaggc tgtcccggtc
     4741 caggtccgag gggaggcccg ggaatacgag gacatgatcc cccgcggcca gacgggcctt
     4801 ggcctcccgg gtgatcatgg cccggccctg gtacacggtg acggaaagga cgacggattc
     4861 gacttcggtg atggtcatct cggcgcgcct cgacgattca ggatgaactc aagtgtaacg
     4921 cgcccgaacc ggtgaagcaa atggaacggc cgccccggag agttcccgaa ggcggccgta
     4981 cttggattct cggcaaatca ccggaacagg gaatgtccct ccttgttcag ggcctggagc
     5041 tcctccacga tcttgagtcc cttgggggcg tcccgttcgc ccgcggcccc gacgcctcgc
     5101 agggcggtat ccacgaattt ccccaggatc cggtcccact cggccttgct gcccttgggg
     5161 ggggtgaact gcaggatccg gttctgtcct tccgcgaact tccagaatcc ctcggctgcg
     5221 gcgtagaaat ctcccttgtt cagggccgcc cggacctgac ctagggtctg caggttggtc
     5281 cgcatcacct ggaccactac gtcccggtcg taggcggact gggccgcggc gggcagggcg
     5341 agggtcaggg caaatgcaac cacgatcacc ccggcgaggg gcagggatct caaccgtttc
     5401 aagacgacct ccttcgatac gtaaagatta tagaaaaagt aatataacca tgttgggaag
     5461 gcaaatgatt catgcagggg ccggttttcg ttgacagatc cggaaaaggg gtggatactc
     5521 atcttaactg gttaaccact tttgttaacc atacggcaaa gaagggcgtg tggaatgtcg
     5581 gacgttaatc cggacagcga gaggatcgtt cttgagcatc tccgcgacca gatcgtctcc
     5641 gggaagctga aggcgagtac gaagctgccc tccgaacgcg ccctgtgtca gaccctgggc
     5701 gtaagtcgcg gttacgtccg gaaagccctg gccaagctgg aacactacgg gttgatacga
     5761 acccttcccc agaggggcac gatcgtagcg gggctgggca gcaaggccat ctcgggcttg
     5821 atcgccagca tcggcagcct ggacgaggac ttcgggccgg tggagttgtt cgagatccgg
     5881 gcactcctgg aaaccttcgc ggcccggaag gctgcggagc gggcgggggc ggacgatatc
     5941 acagaaatcc tgaagtggca ttcagaattc cggatccggg cggatgccgg acaacggggg
     6001 ctggaggagg atcatctctt ccacctggcc atcgcacggg ccagcggcaa cccggtatgc
     6061 ctggccctga caagctactt caccccccag atcatttcgc tcaacgcgga ttacgcggag
     6121 agcgacccgg accgattccg acgaaccttc gcggaacacg acgccatcgt ccgggcgatc
     6181 ctggccgggg atcccgacgt ttccgcggag gcgatgcgga gccacatgga ggaagcctgg
     6241 aagcggaggc ttcccgaaag atcgccccgc tgaacgtggg ggcacgaggc aagggtaggc
     6301 ggccggtacg tatagatcgg ccatgtatca aacgtcccga aggagggaac agtcatgaaa
     6361 aaggtattcg cgctggccgt cgtcctgtcg atgatcgcgc tggccgtcgc cgcggctccc
     6421 aagccggtca ccatcaccca ttggtactgg gcggacaatc ccaagtactc cgcggtcatg
     6481 aaggaaatgg cggcggactt caacgcgacc aacggcaagg ggatcaccgt ggtggtccag
     6541 gaatatccct gggacggcgg aggatacagc gagaccctct tccgggccgc gatgggcggg
     6601 ggcgggccgg atacctcctc ggccaagctg acctcgactc ctctcttcgt gtcgaacaac
     6661 ctgtgggaaa atctggacaa atacatcaac cgctggaagg acaaggacca gatcgatccc
     6721 gccctctgga agaccatgcg ggatgccagc cggaccaaga acgtgttcct gatgccctgg
     6781 accacccagg tcctgtacgt gtactaccgg ccctccatgt tcaaggccgc ggggatctcg
     6841 gtgcccaaga actacgccga gttcctggac gcctgcgcca agctgaccaa ggacaccgac
     6901 ggggacggcc gaacggacgt atacggtttc gggatgcgcg gcttccgggg aggacacgag
     6961 ccatggggga gcttcatcca ggcccggggc gggaccttcg cggactttac caccgccaag
     7021 gccgtggcgg ggatgcagga cttcatcgac ctcttcaaga agggttacgt accgccgacc
     7081 gcgccgatgg acgggttcaa cgagatcatc gccaacttca agtccgggaa gaccgccatg
     7141 acggttcacc acatcggatc ctccacgggc atgatcgaca ccttcgggga tgacgtggac
     7201 gccttcccct tccccggcgc cgagggcaag tggacctcca tgggggacac cgagaacgtg
     7261 atgttccggt ccagcaagaa caaggacgcg gccttcgagt ggctggccta cttggccgcc
     7321 ggcaaggggc agatcacctg gtgcgccgcc accggcaacg tgcccgtgag caaggccgtc
     7381 cagaagctgc cccagttcca gagcagccgg ttcatgaagg tctccatcga gggagccccc
     7441 ttcgccggca tctacccggt cctggacact actactgaat ggatcagcac gatatggccc
     7501 aacacgatcg cggcggccct ccagggcaag atctccgcgg cggaggccat gaagcagctc
     7561 cagaccggac tgtataagta aaggttttca gaatcaccac ggaggtcacg ggatgcacgg
     7621 aggtgtgaaa aaggaaagtt cattctttcc ttcctccgtg acctccgtgc cctctgtggt
     7681 gaaatcccga aaggtgatta tccccgcgac agggatggag aagaatggga cgtaagagaa
     7741 acccctggcc ctacctgctg gtggcgccgg ccctgctcac cgtgctgacc gtcgtgttca
     7801 tccccgtggt gaacgcggtc tccatgagct tccagagcta cgacctccgg cggcccagcc
     7861 gcataggctt catcggcctc gggaattacg ccgaggttct ctcggaccca ctcttctggc
     7921 agtccgtctg gaagaccgtg gtctgggtcg gcgtgggggt gggtttccag ttcctcttcg
     7981 gattcatcct ggccctcctg ctcaaccggc gcttcttcgg ccgggggatc gtgcgttcga
     8041 tcagcctgat cccctgggtg accccggggg tgctcatcgg cctcatgtgg cggtggatct
     8101 acgacgggag ctacggggtc ctgaacgata tcctgctgag gctcgggatc atccgggact
     8161 acgtgccctt cctggcccgg ggggatacgg cctttccggc cgtgatcctg accatcatct
     8221 ggcaaggcat ccccttcttc gccctgatga tcctggcggg cttgcagggg atccccgagg
     8281 agctctacga cgccgcggac gtggacgggg ccaacggtgt gcagaagctg ttccgcatca
     8341 ccatcccgtc cctccggaac gtgatcttcg tcaccaccct gctcaggatc atctgggtgt
     8401 ccaactccgt ggacgtgatt ttcaatctga ccgagggcgg ccccgcctac gcgacccaga
     8461 ccctgagcgt ctacgtcttc aacaagggga attccctgaa cctggggtac gccgcgacca
     8521 tgtccatcct gctggccctg atgctgtcct cggtggcggt ggtgtacctg cgcagaattt
     8581 tccggaacca ggaggccgcc cgatgaaacg atcccccgcc ggcgcggcca agaacctggt
     8641 ttcctatctt ctcctggccg ggatgctggt cttcctgctc ttccccttct actggacctt
     8701 cgtaacctcg atcaagccgg agagcgaact gtacggaaag accgtcacct actggccccg
     8761 gaaagccact ttcgaatcgt acacgaggct attcagcgac tacaatttca tccgccccat
     8821 gacgaacagc ctgatcgtcg cggcctccac caccctggtg tccctgacgg tctccctgct
     8881 cgccgcgtac tccttttccc ggttccagtt caaggggcgc aagttcctca tgaccctgtt
     8941 cctgacgaac aacatgtttc cgacggtcct cctcctgatt cccctgtacg ccatcatgag
     9001 gaagctcggc atcctgtata cccctttcgc cctgatcctg gcgtacacga ccttcacgat
     9061 ccctttttcc atctggctgc tcaacgggta ccttgcggac cttccaccct ccctggagga
     9121 ggcggccatg gtggacggcg ccaaccgcgc ccaggccttc gtgcgcatcg tgcttccggt
     9181 cctgatgccc tgcatcgtgg cgaccggggc ctacgtgttc atgacatctt ggaacgagta
     9241 taccttcgcg gtgatgttca ccaacgaggc caaccgcacc attcccgtgt ccttgaaaaa
     9301 cctcatcgga cagctggggg tggagtgggg aatgctgacc gcgggaggca tcatcaccat
     9361 cgtacccgtc tgtttcatgt tcttcttcgc gcagaaacgg ttgatagaag gcctgaccgc
     9421 cggagcggtc aaaggctgac ggaaaggagt tcccgaacat ggcccacgaa cctcaggacg
     9481 cgacggcgcg tcggatacgc cgcgacattc tcaaaatgct ctacctctgc cagtcgggcc
     9541 acccgggagg atccctctcg tgcgtggaaa tcctggaggg actctactac cggacgatgc
     9601 gggtcgatcc gtcccggccg ggctgggaag accgggaccg cctggtgctg agcaagggcc
     9661 atgcctgccc cgccctgtac gccgtcctgg ccgacctggg cttcttcccc agggaggatc
     9721 tctggaggct ccgccaggtg gacagccacc tccaggggca tccggacatg accaagaccc
     9781 ccggggtgga cgccaacacg ggctccttgg gccaaggact atccatcgcc gtgggcctgg
     9841 ccctggcggc caagcacaag aaaaaagact accgcgtgtt cgcggtggtc ggggacgggg
     9901 aagtccagga gggcatgatc tgggaggcgg ccatggccgc ggcccattac aagctggaca
     9961 acctgatcgt gctcctggac cacaacggct tgcagatcga cggacccaac gacaaagtca
    10021 tgggactggg cgacatagtc ggtaaattct ccgccttcgg attcgactgc gaggtggtgg
    10081 acggccacga tgcccaggcg gtcgccgagg ccgctgggcg acgcgcggac ggaaaacccc
    10141 ggttcatccg ctgccgcacc gtcaagggcc ggggggtctt gttcatggag ggcaacgcgg
    10201 gctggcacgg caagcccatg aacgcccagg agtacgaagc tgcgatgaag gaactcgggg
    10261 gtgcagacca tgtctgacgc gaaatcgctg cgggtggcct acggggaggc cctggtggaa
    10321 ctgggggccc aggacgaccg ggtcgtggtc ctggacgccg acctggccca cgccaccatg
    10381 accagcctct tcgaggccaa gtttcccgac cggttctaca acttcgggct tgcggaagcc
    10441 aacatgatgt gcgccgccgc gggcttcgcc cactcgggac tccttccctt cgcctctacc
    10501 ttcgccctgt tcggaaccgg gagggcttac gagcagatcc gcaactccat agcctacgtg
    10561 aacgccaacg taaagttcgg cctatcccat tcgggcctct ccgtgggcga ggacgggggg
    10621 agccaccaga gcatcgagga catcgccctc atgcgggtgg tgccgaacat gaccatcttc
    10681 gtaccctgcg acccgatcga gacccggaag gccgttttcg cggcggccaa gatcgagggc
    10741 cccgtgtaca tccgggtggc ccgcccggtg gtggagacga tcacgacgcc ccagacgccc
    10801 ttcgttccgg ggaaaatcgt ccggctccgg gacggcacgg acgtctgcat cctggccacg
    10861 gggctcatgg tcaaggaggc cctcaaggcc gcggatctcc ttgcctccga gggcatacgc
    10921 gccgccgtgg cggacgtcca cacccttaag cccttcgacg ccgagttcgt gctggaggcg
    10981 gcccggacct gcggccggat cctgacggtg gaggaacact ccgtgatcgg aggcctcggg
    11041 tccgcgtccg cggaagtcct ggcgggggcc tctgccgcaa aattccggcg gatggggatc
    11101 ggggacaaat tcggattctc gggcaaaccc gcggacctct tcgagtcctt cggactgacc
    11161 gcaggggata tagcccgtga atgcaaggcc ctgctggact agggcggagg acggatcatg
    11221 aacatagaat cccgcgggca cgagggggaa gggctggccc gggtctacga aaacgaccgt
    11281 tggatggtcg cggtcaagaa ctggaagccc gcgaacgacc tcgaaggcat agactgcctg
    11341 gagcgtcaca acgagacgga cgagctcttc gtcctcctgg ccggccgctg tgtcctgctg
    11401 tccgggacgg agaagcccgg aggcggattg gatatccggg cgcattccat ggaacccgga
    11461 aaactctaca cgattccccg gggccttttg cacaacacgg taacccgcag ggacaccaag
    11521 ctcctattag tggaggcccc ggacacgtcc atggcgaaca gcgaggtcgt gagcctggac
    11581 gcggagcaga aggcctccgt gcgggccctc gcgcgcccgg ggttgtagga cgggcatgag
    11641 gatcagcgaa gtcgtcgcgc cccgtcggtc ccgtgtttcg gaggcgacgg tccccgagcc
    11701 cggggcgaac gatatcctcg tccgggtccg ggcctgcggg gtctgcggat ccgagtttcc
    11761 gacctggacg gagggaaggg atccccccgt ccggctgggc cacgaggtcg ccggggaggt
    11821 cgccgcggcg gggacagggg tttccggatt cgagcccggg gaccggatca ccgggctctt
    11881 ccgtcaaggt ttcgcggagt acgcggtctg tccggccgag gcagccgcgc ccattccggg
    11941 tggaatgggc tacgaggagg ccagtctcgg cgaaccggtc gcctgcgccg tgagcggggc
    12001 cctgcggacc gacgtgggcc tgggaaagac cgtcgcggtg atcggcctgg gcttcatggg
    12061 actgctcacg ctgcagctgc tccgcatgaa gggggcgttc cggattttgg cgatcgatgc
    12121 gcggcccgag acgggggcgg ccgcccgtcg cctcggggcg gacgagttct tcctgcccgg
    12181 ggaggtaccg ccggggcttc tgctgacgga cagcgcgggg gccggcggag tggacgtggt
    12241 cgcggagtgc tccggtagcg gcgcagccct ggaactggcc gtccggatgc tcaagcggca
    12301 ctcggtcctt tccgtggtgg ggtaccatca gggcggaccg agatcggtgg atttccagat
    12361 gctgaactgg aaggccgcgg acgtgatcaa cgcccacgag aagcggacgg acttcaagat
    12421 ggaatgtctg cggatcggcc tggacctggc ggctgcgggc cggttggagc tgggatccct
    12481 gatcacccac cggtatcccc tggaagaggc cgaccgcgcc ttcgcggact acgagtcgaa
    12541 gccggacggc tatatcaagg gtctggtact gccctgaggg gcgtcgggag gcggcatgag
    12601 gcgtattcga gccgggatcg tcggcgtcgg caagatcggg ctggtccatc tggaagccct
    12661 tcgacgattg gggtatgccg aggcggccgc cgtggtcgtg cgggacgaag cccgggcgaa
    12721 ggcgctctgc gagttctacg gcatcccgaa gtactatctc gactacaggg acctgctggc
    12781 cgatcccgga atcgaggtcg tccacgactg caccccgaat cgtgaacact tccggatcaa
    12841 tggggacgct ctccgggcgg gcaagcccgt cctttcggaa aagcccctca ccgtggattc
    12901 ccgggaatcc gcggaactgg tcgaagtggc ggaagctacg ggcctccgca cgggggtcaa
    12961 tttcgtgtat cgccattatg cggtggtcca acatctccgg ggcatgatcg aggagggccg
    13021 actcggggtg gtccgggcgg tccacggcga gtacctccag gattggctcc tcaaggagac
    13081 ggatttcgac tggcgggtcc ggtccgagat aggcggaccc tcccgggcat tggccgatat
    13141 cggatcccac tggctcgacc tggcgatctt cctcaccgga tgcccgatcc gggaaatcag
    13201 cgcggacacg gcgaccttcc tgccgttccg ggtggaaccg gacccgtccg gcaaggggga
    13261 aggtcgaagg gtgccggtgg atacggagga ttacggggcc gccctcctgc gcttcgaggg
    13321 cggggtccgg ggagtcttcg ccgtatccca ggtcagcccg ggccggaaga tcggcctgtc
    13381 catccaggtg gacgggagtc tcgcatccgc ccgctggtgc caggattctc cagaccggct
    13441 gtggatcggc cggcgggacg ggcccaacga ggacttcgtc ctgcatcctt ccttgttgaa
    13501 tcggcgcgga aagtccgtgc actcgtatac gggcggtccg gagcggtggc cggacgccca
    13561 gaagaacatg atcgacagtt tctatcgcac gatcctggac ggggtggagc ccttgtacgc
    13621 ggatttcaaa gccggccact ccgttgtccg cgtagtggaa tccgcgctcg agagcggccg
    13681 gaccggatgc cgggtcacgg tcgccggata ggaatgccgg attccggcgg catcaggagg
    13741 cgcggaggga ccgaagggcg tcctcgatgt ccttaggcat gaggggtttg accaggaagg
    13801 ccgcgggctc ggttccggcc gccgccgcgg cggtatccga gtcgtcgtag gctgttgtga
    13861 agatcaccgg ggcgccgccg gtttcccgga tcttccgggc ggcctcgatc cccgacatgc
    13921 gggtttccat gcagatgtcc atgaacacca agtccggccg cagttcgcgt tctttctgga
    13981 cacaggaaac ccccgaagag cagatggcct gcacctcgtg cccgagttcc cggagcaccg
    14041 cggccaggcc catggcgatg agacgctcgt cctccgcgat gatgatcctc atcgtcccaa
    14101 ggcctcccga gaaaggcgca cctcaacccg cagcccgggt tgtccgttcg tccaacgaac
    14161 atccccgtag aactgctcgc acaggctccg gacaatggac atgcccaggc tctcacggtc
    14221 cgtacgctcc gaatccgtcg acagtccggg gccgtcgtcg gtgagggcaa tatagcaatt
    14281 cgactcatcg ctgtcgaccc gtatatccat ccgtcctgta gtactgcccc ggaaagcgta
    14341 tttgacggaa ttggtgatca gttcgttcag gataaggccg atgtggatac ccgcgctcag
    14401 gctgacccga agggtcccca tggaaaaggt cggcacgata ccgcacccgt ggctctccag
    14461 gatggatcgg gtcagccggg ggatataccg ctccagatcc accgaggaaa ggtcgtcggc
    14521 ggaatagagc tcctcgtgga ccagggccat ggaggagatg cgcccccggg ccgtgagcag
    14581 ggccggggcg gccggtgatc cggcgttctg ctggccctgt aggtacagga ggctttcgat
    14641 gatctgaagg ttgttcttga cccggtgatg gatctcctgg agcaggacgt tcttttcctc
    14701 caaggccgag cgaagctgct cgcgttccgc caggaggtcc tcggtccggc gacgcaccgt
    14761 gtcctcgagc ttgaggtaca tgaaggagtt ccgcaggacc agggccagga ccccggacaa
    14821 tcgatccagg ctgacgagca ggttgcccat gctgtggcgg tccaggatgt ccaggagtag
    14881 gagggcccct acctgtcctc cgccgtaccg caggggcacc aggatgctgg ggccgaatcc
    14941 cgcacctcgg agcgcatcgc gcacggcggc atcccgagga tcgccggcat cctctccggg
    15001 atcgacgaat cgggaagcgg cgaacccgtg cgcggcttcc aggagcgtga gtatctcccg
    15061 ggattccgcg gtcgttctcc tccggtccgg gaccgcggag atgagctcgt gggcatggga
    15121 atcgctgaac tcggagcatt ggaacaccag caccgtcttg accccgacga tctcccggat
    15181 ccggccggcg agatgggtgg aacaggcccc gggattgtcc gaccggctca ggatctcctc
    15241 caggaggtcc gcgaggagga aggaaagggc attgttctcg gatccg
//
LOCUS       DAOTEI010000075        15281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2657_length_15281_cov_5.913109, whole genome shotgun sequence.
ACCESSION   DAOTEI010000075 DAOTEI010000000
VERSION     DAOTEI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15281
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2657_length_15281_cov_5.913109"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="P5313_08755"
     CDS             <1..271
                     /locus_tag="P5313_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80492.1"
                     /translation="LLYDKDGRCVNPRFLDYKIPTIWEVPEDFHVELVPVADEVGPFG
                     GKSVSEISLNGAAPAISIAIHDAVGVWMREWPFTPERILKALGKI"
     gene            429..1622
                     /gene="ygeW"
                     /locus_tag="P5313_08760"
     CDS             429..1622
                     /gene="ygeW"
                     /locus_tag="P5313_08760"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HRY80493.1"
                     /translation="MNLNEIRARIETLKNRKIGMYRKDFLLTWEKTRDEIDATFDVAD
                     ILRGLRDAGISPRVWDSGIAVSNFRDNSTRTRFSYSSACDLLGLYVQDLDEGKSQIAH
                     GETVRETANMISFLTEAIGIRDDMYLGEGDKYQREVGKALDDGVAAGVLPDRPTVVNL
                     QSDIDHPTQSMADFLHLAHHFGGLSKLKGKKIAMTWAYSPSYGKPLSVPQGIIGLASR
                     FGMDITLAHPEGYDLIPEVVELSKKQAKESGGSFKHVNSMDEAFQGADIVYPKSWAPY
                     DVMLKRVPLLHAGDQAGLKDLEKACLANNAKFRDWECTEQKMKLTAGGKALYMHCLPA
                     DITDVSCKAGEVAATVFDRYRDDTYREASWKPYIIAAMILLGKFQDPAKVLAGLVERN
                     RKRVG"
     gene            1749..2654
                     /locus_tag="P5313_08765"
     CDS             1749..2654
                     /locus_tag="P5313_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY80494.1"
                     /translation="MAKCANLACAGLLVAVCALGCVSDEREPPTPWSRWTEIDGTPVH
                     YIDTAPGSDLPALLIVPGYLGSGATFRNFISLLQPGFRIVLPEMPGFGYSPPPPGPCS
                     LDRMTKIVEGVVLEAGLENISLIGSSMGSLIAVRLAVDRPDRIDRIVLLSPFGAGGQA
                     DVAARLDRLNFLLPAASVLARKQIVARQIRKQVRDPAELTPEMIDCYARPFRTREGRR
                     LVVEITRDVIGECTFDDRLPLVRQPVLVIAGTEDRLMDSATLTMLQTRVPRCSILRLE
                     GCRHLVYLDAPAQVAELVVAFCERE"
     gene            2668..6912
                     /locus_tag="P5313_08770"
     CDS             2668..6912
                     /locus_tag="P5313_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRY80495.1"
                     /translation="MERYSLGLDIGSISVNTVLIDEDRRIVENHYTLCQGSPFSALLG
                     VLEGLLERFPPGALVRVATTGTGGKLAARLVGGAFFNEIVAQSCSVGALHPEARTVIE
                     IGGEDSKLIMMREGRAEDSSRLSDFAMNTVCAAGTGSFLDQQARRIGVSIEEFGNLAL
                     ACERPPHIAGRCSVFAKSDMIHLQQIATPLEDIVAGLCFAVARNLRSNLARGRKLRSP
                     VVFQGGVAANAGVVRAVREVFGLRDGQLVIPEYHASMGAIGAVFRLLDAAPKEDARFH
                     GLEALKAHLDDGKTELPHWPQLVRSRAFGTKATHPLPDTREPVEVFLGIDVGSLSTNV
                     VLIDGDTNVVARRYLATAGKPIEAIRTGLSEIHGEVGDRVVVRGVGTTGSGRYLTGHF
                     TGADTIKNEITAQATAAIHFHPEVDTVFEIGGQDSKFISIDHGVVVDFEMNKVCAAGT
                     GSFLEEQAEKLGVNIVDEFGERALSADRPCRLGDRCTVFMESDLNSRQQKGAGKDDLI
                     GGLAFSIVQNYLQKVVGSKRIGDHILFQGGVTNNPAVVAAFEAVTGKPITVPPHFDVT
                     GAIGAAMLARDEVRMTGRPTRFKGFGVSRVPYAMSRFACAGCENRCDVHKVVIEGESR
                     ALYYGDRCDKYQPPDQRREKELRREGIPDLFKRREDILLGSFRDEPRDPARTSIGIPR
                     ALMVYYQQFPYWRTFFESLGFQVVLSRPSDRPLVTKSLGLLAAETCFPVEVMHGHVHD
                     LLERGIDYVFAPFVVDNEAAAGSQSVNYNCPWIQCYPYMIRSSFGDRETRGKFLVPTV
                     HFRYSRRKLAADLSRFMEETFGTPPRDTVRAIGAAEAAQRSFLRAVSEEGRRTLADLP
                     PDRNAFVVLGRPYNAGDPELNLGLVKKLIDLDVMPIPLDFLPLEQELGGVHRDYDMVC
                     WPNAQRILAGARIVARSDRLQAVFITNFRCGPDSFLSHYLREELRGRPHLQLEVDEHS
                     ADAGMITRCEAFLDSLKAHRSGVPSSLRSGAIWRFTTTPSAGRTLYFPYMCDAAHLLA
                     AAARSCGIDAEVLPMQDETALDWGRRYTSSRECFPMICTTGSFLKKLAEPGTDPRRTS
                     FFMPFHGGPCRFGQYQKLQRIIASNLGYGDVEIVSPSNKKSYADFTSGRGVKFRLVVW
                     KGLVACEILARLRQERKPYEARPGQTEETYRRELDRLARCLEGGARDIVRQLRGSVEA
                     FDAIPLLDMPRKPVVAVLGEIFMRDNPFCSGFLRDRLEELGAETVMAPVREWLELSSV
                     NYRKVSLWEGRYLDAAKARVQLFFQERIAHGIERALSDTFDANRIVPIEDILDLCGTY
                     IHRDYSGEPPLALGAAASLARTGVSGFASVIPFTCLPGTVVASLSDSFRTDHGDIPWV
                     NIAFDGQEDIGAETRLEAFVYQAREYARAHGYDGVR"
     gene            6967..9207
                     /locus_tag="P5313_08775"
     CDS             6967..9207
                     /locus_tag="P5313_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY80496.1"
                     /translation="MGSVRRRFLKRYFVEIWLLLIASIAVTILALSAFLYKSFESATV
                     ATVYRYNRESLLETGRINEYVRKMIRTSGMELFSEPSIRRLLYGRDLTNFETLSGVRR
                     LDSAASTGRYIHSIYVYNAPAGYVYSSSDTVSDGLDRFEDGRLVEILEASGDRPGLVP
                     VARYIEGSSRTVPVHSFIFHSTSAFAPDARSALIINVTLDWLREIVRWGSSRLYMLDA
                     SGKVLYHDEADLFLSDLSGESFVRRVLESGQESGAFVDMVSGVRSLVLHAASGDLFLV
                     RTFPYAEVMRDLGSLRTTTAFSAFGVLAFGMLVAFAVSRRLYRPIGKLSLAIGSALTP
                     GSASPEQDEVGRISSAIESMVARTRTIEESSRARSEALAQEVLKEFVQGETLGFPGAG
                     ELFREYGVPFDPDGAFRLVAVRPDRTDPSHGSTVPRGLPSRSGNHGSACVLSVDGVEL
                     AVLQGETASREAALVQSWKARTSGVVAVSGRLPRADAIPAAFSRLRDRIRMGFLSSPG
                     EVVRLSDEEEPETGLEYPSEAERRILQSLRAGDSSAAEDALESFLDALSGKRFDPFRF
                     ALRRLYASVRELERELRSDPGPAGSDLDGLPSEPTSLEEILDPFRNLFNSVREALEEH
                     RAARCRELAEAVRSILRVRYTDPNLTLRSIADELGYSSSHVARAFKEGTGSSVADAIL
                     EYRLEAAKRLLLEDGCPAKDIAARVGLVNENYFYTLFRKKVGLTPAAYRRGGLQNPAE
                     TGNAGI"
     gene            9279..10307
                     /locus_tag="P5313_08780"
     CDS             9279..10307
                     /locus_tag="P5313_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY80497.1"
                     /translation="MRNMKRKALFAAAAVLALAGCGSRSAKSEGTAAGGGRQSVMIYS
                     SLKESQLAALKEGFMKRHPNIAMDFYTASTGNVMTKLATEQQAGGIAADLIWVGDPTN
                     YIDFKKQGLLYAYDSPAAADIPAKFKDPDRMFMSARLIMLGFVYNTRRLTYEQAPKTW
                     EDLLNPRFRNNIAMTDPTSSGTTLFTVAGLVQNPRYGWDFLRKLKAHGMLLESGSSAV
                     VNKVGSDEYLVSVGVDYIARSAMAQGATLGFVYPERDIPIIESPIAIIRNSKNLDAAK
                     ALYDYIISEEGQQVLLEEYTLPINPKMRLDNAIEVAEAEKRMLPVDHVALVTDKNSLL
                     EKFDSIFK"
     gene            10405..11562
                     /locus_tag="P5313_08785"
     CDS             10405..11562
                     /locus_tag="P5313_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80498.1"
                     /translation="MSSVQIDAISVDYGKHRVLDKLSLEVRDGESLAVVGPSGCGKTT
                     LMRALCGFIRTAEGQISIDGRAVSDSASRQFTAPEKRNIGVVFQDYAVWPHLTVIENI
                     SYPLKKRRLPKSEIRERALKALGQVKMAGYENRLPAQLSGGQQQRVALARALVTSDRL
                     IVLDEPITNLDAKLREEMAYEIRSLQSSAGVTILYITHDQETAMTIGDRMVIMDRQGA
                     LRQVGSPEEIWTSPADKYVFEFLGVSNFLPVTVRKDRLIVRDGDGFQEAALAGGRIAG
                     QALEGLDDVLLAVRPMDVVLHPRPKSGLFSGRVERVTYLGNQFDYIVALGEHRLRVQQ
                     DSFDAFARGVPGEGSTCGLDLGNSVFYRAGGSERVDLAGTRAGGTRQRTEA"
     gene            11559..13283
                     /locus_tag="P5313_08790"
     CDS             11559..13283
                     /locus_tag="P5313_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY80499.1"
                     /translation="MNAFGTKPGALPGTAGRRGLGLDFGLYILSTAILVITVLLPVLM
                     IAYNTFIDGGRLDWRMFVRVVGDPGNLKSVWNTVVIALWVTVLGTVLGIFYAWLIGRS
                     DIPLKGLMKSLFTIPYMFPPFFGAMAWSLLLAPRSGYINRLFMDLTGGTTPLFNMHSI
                     AGIVFVETCYYFPFVFLQVVSALERMDPTLEESARIAGAGQWYVIRRITLPLVLPAIA
                     AGAMLILISSLSHFGVPAILGFSQNIFTLPTKIYQLIRRSAGSFQGIREAAALSILLI
                     VVVVAALYVQKRVLKTGRYDIIKGKSMRPMLIKLRGARLPILAVCLASLGVIVVLPLA
                     VVILVGFLKAYGLPFRFENFTIRNYQYVLFRSRMVIDSIRNSLFLSVASGVITMFLGV
                     MVAYVVVKVKPRGKGLLEILSLLPYSMPGIVLAIGVILVWSGAFYLNLYNTIWIILIA
                     YIARYVSFAMKSASASLEQVHYSLEEAARSCGATHLESLRDVTLPLIRPGMVAGFFLI
                     FLPGMRELTTSVLLYGPFTRTLGVAIYSMNAEGNTVQSSALATIAIGIILAGNLALRW
                     VTKDRKGA"
     gene            13285..14391
                     /locus_tag="P5313_08795"
     CDS             13285..14391
                     /locus_tag="P5313_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80500.1"
                     /translation="MSRIQLKGVSKVFPTRSGASVAAVKDLDLVIEEGECFSFLGPSG
                     CGKTTTLRMIAGFEDLSAGEIWLGDRAVSIKERNLYIPPEQRGLGMVFQSFAVWPHLN
                     VFENVAFPLRVRDLPRTDIAEAVAQALRHTDLAGLEGTFPADLSGGQQQRIALARAIV
                     TKPRVMLLDEPLSNLDPKLRESMRFEIKELQRKLNFTIVFVTHDQAEAMALSDRMLVM
                     DLGKVQQADTPEALYNRPASRFVYGFLGLSNFTDVELRGGDLYVPGSKYPLPMELPAG
                     AAREGVMATRPNEITLSPKDEGGCPAVVVKRLYLTYCIEYQARVGDQTLRVHCPHSIV
                     LEEGRPCFLRFKNPRWYPRESGRAEIERNERQVI"
     gene            complement(14450..>15281)
                     /locus_tag="P5313_08800"
     CDS             complement(14450..>15281)
                     /locus_tag="P5313_08800"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY80501.1"
                     /translation="IIPPSIPFVIYGVITGTSIGRLFLGGIVPGILIGLVQMVISYFI
                     CAKRGMGSPKRFSIRDFLGSLRRGILALFMPVIILGGIIFGIVTPTEAAVLAIVYALF
                     VSMVVYRELKPRDLPKLIYESGITTAIVMFMIAGAFLYSWIITNDQIPQKLAALIGML
                     TQDKNVVLLLFVVVYLVAGMFIDLGAAMILLVPVLYPVTRMMGIDPILFGIVTVIALA
                     IGLVTPPVGACLFIASEIGGISILKTARSAIPYLLGILLVLLAIILYPPLALFIPGLM
                     "
BASE COUNT         2702 a         5096 c         4842 g         2641 t
ORIGIN      
        1 gctgctctac gacaaggacg ggcgctgcgt gaacccgcgg ttcctggatt acaagatccc
       61 cacgatctgg gaggttcccg aggacttcca cgtggagcta gttcccgtgg cggacgaggt
      121 gggacccttc ggggggaaga gcgtttccga gatctccctg aacggggcgg cccccgccat
      181 ctccatcgcc atccacgacg ccgtcggcgt atggatgcgc gagtggccct tcacccccga
      241 gcgtatactg aaggctctcg ggaaaatcta ggtttcagga taagagagac aggatgaaca
      301 ggattaggag aggatgaaca ggatgaaagt gagagagaaa gaagagggga tataattcat
      361 ccctttgagc aatcctgtgc atccttcttc catcctgtcc atcctgtcca gaatttaggg
      421 aggaacccat gaacctgaac gaaattcgcg cgcgcatcga gaccctgaag aaccgcaaga
      481 tcggcatgta ccgcaaggac ttcctcctga cctgggagaa gacccgggac gagatcgacg
      541 ccaccttcga cgtggcggac atcctccggg gcctgcggga cgccgggatc agcccccggg
      601 tctgggactc cggcatcgcg gtgtccaatt tccgggacaa ctccacccgc acacgcttct
      661 cctactcctc cgcctgcgac ctgctgggcc tctacgtcca ggacctggac gagggcaaga
      721 gccagatcgc ccacggggag accgtgcggg agaccgccaa catgatctcc ttcctgaccg
      781 aggccatcgg catccgggac gacatgtacc tgggcgaggg cgacaagtac cagcgggagg
      841 tcggcaaggc cctggacgac ggagtggccg cgggagtcct gccggaccgg cccacggtgg
      901 tgaacctcca gagcgacatc gaccacccca cccagtccat ggcggacttc ctccacctgg
      961 cccaccactt cgggggcctt tccaagctca agggcaagaa gatcgccatg acctgggcct
     1021 actccccctc ctacggcaag cccctctcgg tgccccaggg gatcatcggc ctggccagcc
     1081 gcttcggcat ggacatcacc ctggcccacc ccgagggcta cgacctcatc cccgaggtgg
     1141 tggagctgtc caagaagcag gccaaggaat ccggcggctc cttcaagcac gtcaactcca
     1201 tggacgaggc cttccagggg gcggacatcg tgtaccccaa gagctgggcc ccctacgacg
     1261 tcatgctgaa gcgcgtcccc ctcctgcacg ccggggacca ggcgggcctc aaggacctgg
     1321 agaaggcctg cctcgcgaac aacgcgaagt tccgggactg ggagtgcacg gaacagaaga
     1381 tgaagctgac cgccgggggc aaggccctgt acatgcactg cctgcccgcg gacatcacgg
     1441 acgtctcctg caaggccggc gaggtggccg ccaccgtctt cgaccggtat cgggacgaca
     1501 cctaccggga ggcctcctgg aagccctaca tcatcgccgc catgatcctg ctcgggaagt
     1561 tccaggaccc cgccaaggtc ctggcgggcc tggtggagag gaaccggaaa agagtcggtt
     1621 aacgtcaagc tataccggag cggcggccgc ggattgcggc cgccgctttt taattttcca
     1681 gcttgcgcgc tccggctgcc gggagtatga ttcgagtata caagtatcca tgaaaaaggt
     1741 gtgcggccat ggcaaagtgt gcgaatctcg catgcgccgg gctgcttgtc gccgtttgcg
     1801 cactcggctg tgtctccgac gagcgcgaac caccgacccc ctggtcccga tggaccgaga
     1861 tcgacggtac ccccgtccat tacatcgaca ccgcgcccgg ttctgatctg cccgccctcc
     1921 tgatcgttcc cggatatctc ggttccggcg cgaccttccg gaattttatt tccctattgc
     1981 agcccggttt ccggatcgtc ctgcccgaaa tgcccggctt cggctattcg ccgccccctc
     2041 ccggaccttg ctccctcgat cgcatgacga agatcgtcga aggcgtcgtc ctggaagccg
     2101 ggctggaaaa catctccctg atcggaagct ccatgggatc cctcatcgcc gtccgcctgg
     2161 cggtcgaccg ccccgaccgg atcgaccgga tcgtgctgct gagtcccttc ggggcgggcg
     2221 gccaggcgga tgtggccgcg aggctcgaca ggctgaattt cctgcttccg gcggcctccg
     2281 tcctggcccg taagcagatc gtggcccggc agatccggaa gcaagtccgg gaccccgccg
     2341 aactcacccc cgagatgatc gactgctacg ccaggccgtt ccgtacccgt gagggacggc
     2401 ggctggtcgt cgaaataacc cgggacgtca tcggcgagtg caccttcgat gatcgcctgc
     2461 ctcttgtccg tcaacccgtc ctggtcatcg cgggcacgga ggatcgcctc atggactccg
     2521 ccacgctaac catgctgcaa acgcgcgtac cccgctgctc gatcctgcgc ctggaaggat
     2581 gcaggcacct cgtgtatctc gacgcgcccg ctcaggtggc ggaattggtt gtcgcgtttt
     2641 gcgaaaggga atgaaaagga ggatacgatg gagcgctatt ccctgggact ggacatcgga
     2701 tccatctccg tcaacaccgt gctcatcgat gaggaccggc ggatcgtcga gaaccactac
     2761 actctatgtc agggaagccc attctccgcc ctgctgggcg ttctggaagg cttgctcgag
     2821 cggttccctc ccggcgccct ggtccgggtg gcgacgacgg gtaccggcgg caaactggcg
     2881 gcgaggctgg tgggcggggc gttcttcaac gagatcgtgg cgcagtcctg ttcggtcggc
     2941 gcgctccacc cggaagccag gaccgtgatc gagatcggag gcgaagattc caagctcatc
     3001 atgatgcggg aaggtcgagc ggaagactct tcccggcttt cggatttcgc catgaacacg
     3061 gtctgcgcgg ccggtaccgg aagcttcctg gaccagcagg cccggcggat cggggtatcc
     3121 atcgaggagt tcgggaacct cgccctggcc tgcgagcgcc ccccgcacat cgctggacgc
     3181 tgcagcgtgt tcgcaaagag cgacatgatc cacctgcagc agatcgcgac gccgttggag
     3241 gacattgtgg cgggcctatg cttcgccgtg gcccggaacc tccggagcaa cctggcacgg
     3301 ggccggaaac ttcgatcccc cgtcgtgttc cagggaggcg tggcggcgaa cgccggggtg
     3361 gtccgcgccg tccgggaggt cttcgggctc cgggacggcc aattggtcat acccgagtac
     3421 cacgcgtcca tgggagcgat cggcgcggta ttccggctcc tcgacgcggc gccgaaggag
     3481 gacgcgcggt tccacggtct cgaggccttg aaagcccacc tcgatgacgg gaagaccgaa
     3541 cttccgcact ggccccagct ggtccggtcc cgggctttcg ggaccaaggc gacgcatccg
     3601 ctgccggata cccgggagcc ggtcgaggtg ttcctcggga tcgacgtggg ttccctgagc
     3661 accaacgtgg tcctcatcga cggggatacc aacgtagtcg cccggcgcta tctagccacc
     3721 gcggggaagc ccatcgaggc catccgcaca ggtctctcgg agatccacgg agaagtcgga
     3781 gaccgggtgg tagtacgagg agtcggcacg accggatccg gccgatacct gaccggccac
     3841 ttcacgggtg cggacaccat caagaacgag ataacggccc aggcgacggc cgccatccac
     3901 ttccatcccg aggtggacac ggtcttcgag atcggagggc aggattccaa gttcatcagc
     3961 atcgaccacg gcgtcgtcgt ggatttcgag atgaacaagg tatgcgccgc cggtaccggc
     4021 agcttcctgg aggagcaggc tgagaagctt ggggtgaaca tcgtggacga gttcggcgag
     4081 agggccctct ccgcggatcg cccctgccgc ctgggggacc gttgcaccgt cttcatggag
     4141 agcgacctca actcccgaca gcagaagggg gccggaaagg acgacctgat cggggggttg
     4201 gccttctcca tcgtccagaa ctacctgcag aaagtggtgg gctccaagcg gatcggcgac
     4261 catatcctct tccagggggg cgtcaccaat aatcccgcgg tcgtagccgc cttcgaggcg
     4321 gttacgggaa aaccgatcac ggtacctcca cacttcgacg tcaccggagc catcggcgcc
     4381 gccatgttgg cccgggacga ggtccgtatg accgggaggc cgacgaggtt caagggcttc
     4441 ggggtcagcc gcgtcccgta cgcgatgagc cgcttcgcct gcgccggctg cgagaaccgc
     4501 tgcgacgtgc acaaggtcgt catcgagggg gaatcgcggg ccctgtacta cggcgaccgg
     4561 tgcgacaaat accagcctcc ggaccagcgc agggagaagg aactgcgccg ggagggcata
     4621 cccgacctgt tcaagcggag ggaagacata ctgctcggga gtttccggga tgagccccgc
     4681 gatcctgcac ggacctcgat aggcattcct agggcgctga tggtgtacta ccagcaattt
     4741 ccatactggc ggaccttctt cgagagcctg ggattccagg tcgtgctctc ccgtccctcg
     4801 gaccggccgc tcgtgaccaa gtccctgggc ctgctcgccg cggaaacatg cttccccgtc
     4861 gaggtgatgc acgggcacgt ccacgacctg ctcgaacgcg gcatcgacta cgttttcgct
     4921 cccttcgtgg tggacaacga ggcggcggcc ggcagccaga gcgtcaacta caactgccca
     4981 tggatccagt gctaccccta catgatccga agctccttcg gggacaggga gacccgcgga
     5041 aaattcctgg tgcccaccgt ccacttccgg tattcccgta ggaagctcgc ggcggacctc
     5101 tcccggttca tggaggagac cttcggcacg cctccccgcg acacggtccg ggcgatcggc
     5161 gccgcggagg ctgcccagcg ttcgttcctg cgcgcggtct cggaggaagg ccgccggacg
     5221 ctcgcggatc tccctccgga ccggaacgcc ttcgtcgtcc tgggccgccc ctacaacgcg
     5281 ggggatcccg agctcaatct cgggctcgta aagaagctaa tcgacctgga cgtgatgccg
     5341 ataccgctcg atttccttcc cctggagcag gagctgggag gggtccacag ggactacgac
     5401 atggtctgct ggccgaacgc ccagaggatc ctggcaggag cccggatcgt cgcccggagc
     5461 gaccgccttc aagcggtctt catcacgaac ttccgctgcg gtcccgactc cttcctctcc
     5521 cactacctcc gggaagaact gcgcggcagg ccccacctcc aactggaggt ggacgagcac
     5581 agcgcggacg ccgggatgat cacccgctgc gaggctttcc tggacagcct gaaggcgcat
     5641 cggtccggag taccctcctc ccttcgctcc ggcgcgatct ggcgcttcac cactacgcct
     5701 tccgccggcc ggaccctgta tttcccctac atgtgcgacg cggctcacct gctcgcggcg
     5761 gccgcgcgtt cctgcgggat cgacgcggag gtcctgccga tgcaggatga gacggccctg
     5821 gactggggac ggcggtatac ctccagccgc gaatgcttcc ccatgatctg caccacgggg
     5881 agcttcctca agaagctcgc ggaaccaggg acggacccgc ggcggacgag cttcttcatg
     5941 cccttccacg gcggaccgtg ccgattcggc cagtaccaga aactccagcg catcatcgcc
     6001 tccaacctgg ggtacggaga cgtggagatc gtatcccctt ccaacaagaa atcctacgcg
     6061 gacttcacct ccggccgggg cgtgaagttc cgcctggtag tgtggaaggg cctggtggcg
     6121 tgcgagattc tggcccggct gcgccaggaa cgaaaaccct acgaggcacg gccgggtcag
     6181 accgaggaga cttaccgacg ggaactggac cgcttggccc ggtgcctgga gggaggggcg
     6241 agggacatcg tgcgacagct gcgcgggtcc gtcgaggcgt tcgacgccat tcccctgctg
     6301 gacatgcccc ggaagcccgt ggttgcggtc ctgggggaaa tcttcatgcg cgacaatccc
     6361 ttctgcagcg gcttcctgcg ggaccgtttg gaggaactgg gagccgagac ggtcatggcg
     6421 ccggtgcgcg aatggctgga actgtcctcg gtgaattacc ggaaggtcag cctctgggag
     6481 gggcggtacc tcgacgccgc gaaggcccgg gtccagcttt tcttccagga acggatcgcc
     6541 catggcatcg agcgcgccct ctcggacacc ttcgacgcga accggatcgt acccatcgag
     6601 gacatcctgg atctctgcgg tacgtacatc caccgcgact attccgggga gccccccctg
     6661 gccttgggcg ccgccgccag cctggccagg accggcgtct cgggcttcgc ctccgtgatc
     6721 cccttcacct gcctacccgg gaccgtcgtg gcctccctct cggattcctt ccggaccgat
     6781 cacggggata tcccctgggt gaacatcgcc ttcgacgggc aggaagacat cggagccgaa
     6841 acccggctgg aggccttcgt gtaccaggcc cgggagtacg cccgggccca cgggtacgac
     6901 ggggtccgat agacggcccg ccgctttttc tttcgccctg cccgggagcc gtgctacaat
     6961 ccgccgatgg gaagcgtccg tcgccggttt ctgaagcgct acttcgtgga gatctggctg
     7021 ctcctcatcg cctcgatcgc ggtgaccatc ctcgccctct ccgcgttcct ctacaagagc
     7081 ttcgagtccg ctaccgtcgc caccgtatac cggtataacc gcgaatccct cctggagacc
     7141 ggacggatca acgagtacgt ccggaagatg atccgcacct ccgggatgga actcttctcg
     7201 gagccctcca tccgtcgcct gctctacggc cgggacctga cgaatttcga gactctcagc
     7261 ggggtccgac gtctggactc cgcggcgtcc acgggacgct acatccattc catctacgtc
     7321 tacaacgccc ccgcgggcta cgtatacagc agctccgata cggtatccga cggcctcgac
     7381 cggttcgagg acgggaggct ggtggaaatt ctggaagcgt cgggggatcg cccggggctg
     7441 gtccccgtgg cccggtacat cgaaggttcc tcccgtaccg tgccggtcca cagcttcatc
     7501 ttccactcca ccagcgcctt cgccccggac gcccggagcg ccctgatcat caacgtgacc
     7561 ctggactggc tccgggagat cgtccgctgg ggctcctccc ggctgtacat gctggatgcc
     7621 tcgggcaagg tcctgtacca cgacgaggcg gacttgttcc tgagcgacct ctcgggcgaa
     7681 tccttcgttc gccgcgtcct ggaatccggc caagagagcg gcgccttcgt ggacatggtc
     7741 tccggcgtcc ggagcctcgt gctccatgcc gccagcggag acctcttcct cgtgcgtacc
     7801 ttcccctacg ccgaggtgat gagggacctg ggcagccttc gaaccaccac ggccttttcc
     7861 gccttcggcg tcctggcctt cggtatgctg gtggccttcg ccgtttctcg gagactctat
     7921 cggcccatcg gaaagttgtc cctcgcaata ggttccgcgc tgacgcccgg atcggcttcg
     7981 cccgaacagg acgaggtcgg acggatctcc agcgccatcg aatccatggt ggcccggacc
     8041 cggacgatcg aggaatcctc ccgggctcga tccgaggcgc tggcccagga ggtcctcaag
     8101 gaattcgtcc aaggggaaac ccttggattc ccgggcgccg gggagttgtt ccgggagtac
     8161 ggagtgccct tcgatccgga cggggcgttt cggctggtcg ccgtgcgccc ggaccggacg
     8221 gatccctccc acggctcgac cgtcccgcgg ggccttcctt cccgctcagg gaaccacgga
     8281 tcggcctgtg tgctctccgt ggacggggtc gagctggcgg tgctgcaggg agaaacggct
     8341 tcccgcgaag ccgccctggt ccagtcctgg aaggcgcgga cctccggcgt ggtcgcagtc
     8401 tcgggccgcc tgcctcgggc agacgcgatt ccggctgcct tttccaggct ccgggaccgg
     8461 atccgcatgg gattcctgtc ctccccggga gaggtcgtgc gcctttccga tgaagaggaa
     8521 ccggaaaccg gcctcgagta tccctccgag gcggaacggc gcatcctcca gagcctccgg
     8581 gctggggaca gttcggcggc ggaggacgcc ctggaatcct tcctggacgc cctgtccggc
     8641 aaacggttcg atcccttccg cttcgccctg cgcaggctgt acgcatcggt ccgggaattg
     8701 gagcgggaac tccgatcgga tcccggcccc gccggatcgg acctggacgg cctgccttcg
     8761 gagccgacat ccctggagga gatcctggat cccttccgga acctcttcaa ctccgtccgg
     8821 gaagccctcg aggaacaccg ggccgcccga tgccgggagc tggccgaggc cgtccgttcg
     8881 atcctgcgtg tgcgctatac ggatcccaac ctgaccctgc ggagcatcgc ggacgaactc
     8941 ggatactcct cctcccacgt cgcacgagcc ttcaaggagg ggacgggatc ctcggtggcg
     9001 gacgcgatcc tcgaatacag gcttgaggcg gccaagcggc tgctcctgga ggacggctgc
     9061 cccgcgaagg acatcgccgc ccgggtgggc ctggtgaacg aaaattactt ctacaccctc
     9121 ttccgcaaga aggtgggtct caccccggcc gcgtaccggc gcgggggact ccaaaatccg
     9181 gctgaaaccg ggaatgccgg aatttaaact ttccgggcgc ccgattgcgg tctagcctga
     9241 accggtcggg ttccacggga cccgggaagg agcaccccat gcggaacatg aaacggaagg
     9301 cgctgtttgc ggcggccgcg gtcctggcct tggccggctg cggttcccgg agcgcgaagt
     9361 ctgaaggcac cgccgcgggc ggagggcggc agtcggtcat gatctactcc tccctcaagg
     9421 agtcccagct cgcggcgctg aaggaaggct tcatgaagag gcatccgaac atcgccatgg
     9481 acttctacac ggcgagcacc ggcaatgtga tgacgaagct cgcgacggaa cagcaggcgg
     9541 ggggtatcgc ggcggacttg atctgggtcg gggacccgac caactacatc gacttcaaga
     9601 aacagggact gttgtacgcc tacgactccc cagcggccgc ggacattccg gccaagttca
     9661 aggatccgga tcgcatgttc atgtccgccc ggctgatcat gctgggcttc gtgtacaaca
     9721 cccggcggct cacctacgag caggccccca agacctggga ggacctgctc aatccccggt
     9781 tccggaacaa catcgctatg accgatccga cctcctcggg tacgaccctc ttcaccgtgg
     9841 cgggcctggt gcagaatccc cgctacggct gggacttcct ccggaagctc aaggcccacg
     9901 gcatgctcct ggaaagcggg tccagcgcgg tggtgaacaa ggtcgggtcc gacgagtacc
     9961 tcgtatccgt gggggtggac tacatcgccc gctccgccat ggcgcaggga gccaccctgg
    10021 gattcgtcta cccggagcgc gacatcccca tcatcgagag ccccatcgcc atcatccgga
    10081 acagcaagaa cctggacgcc gcgaaggccc tgtacgacta catcatctcc gaggagggcc
    10141 agcaggtcct gctggaggag tacaccctgc ccatcaaccc gaagatgcgg cttgacaacg
    10201 cgatcgaggt ggccgaggcg gagaagcgca tgcttccggt ggatcacgtc gccctggtca
    10261 cggacaagaa ctccctgctg gagaagttcg acagcatctt caagtaagcg acccttccgg
    10321 cccggccgcg tcccgccctg ccaaggacgc ggccggggat ccgaatcctt tgcggccgtg
    10381 aggccgcgtt ccaaggagcg tcccatgtcc tcagttcaaa tcgacgcgat cagcgtggac
    10441 tacggaaagc accgcgtcct ggacaagctg tccctcgaag tccgggacgg ggagagcctg
    10501 gccgtcgtgg gcccctccgg ctgcgggaag accaccctga tgcgggccct gtgcggattc
    10561 atccggaccg ccgaggggca gatctccatc gacggccggg cggtgtccga ctccgcatcg
    10621 cgccagttca cggccccgga aaagcggaac atcggagtcg tcttccagga ctacgcggtg
    10681 tggccccacc ttacggtgat cgagaacatc tcctatcccc ttaaaaaacg gcggcttccc
    10741 aagtccgaga tccgggagcg agccttgaag gccctgggcc aggtcaagat ggccggctac
    10801 gagaaccggc tgcccgccca gctttccggg ggccagcagc agcgggtggc cctggcccgg
    10861 gccctggtca cctcggaccg gctcatcgtc ctggacgagc ccatcaccaa cctcgacgcc
    10921 aagctgaggg aggagatggc ttacgagatc cggagcctcc agtcctccgc gggggtgacg
    10981 atcctgtaca tcacccacga ccaggagacc gccatgacca tcggagaccg catggtgatc
    11041 atggatcgcc aaggcgccct ccgtcaggtg ggaagcccgg aggagatctg gacctccccg
    11101 gcggacaagt acgtctttga attcctgggg gtctccaact tccttcccgt caccgtccgg
    11161 aaggaccgtc tgatcgtgcg ggacggagac ggattccagg aggccgcgtt ggcggggggg
    11221 cggatcgcgg ggcaggccct ggaagggctc gacgacgtac tcctggcggt gcgtcctatg
    11281 gacgtcgtcc tgcacccccg ccccaaatcc ggcctcttct cgggccgggt ggagcgggtg
    11341 acctacctgg gcaaccagtt cgactacatc gtcgccctgg gggagcatcg cctccgggtc
    11401 cagcaggact ccttcgacgc ctttgcccgg ggcgtccccg gggaggggag cacctgcggc
    11461 ctggatctgg ggaattcggt attctaccgg gccggcggct cggaacgcgt cgacctggcc
    11521 ggcacccgcg cgggcggaac ccggcaaagg acggaagcat gaacgcattc ggaacgaaac
    11581 ccggagccct ccccggcacg gccggacggc gggggctcgg cctggacttc gggctgtaca
    11641 tcctttccac ggccatcctc gtgatcacgg tgctcctgcc cgttctgatg atcgcctaca
    11701 acaccttcat cgacggcggg cgcctggatt ggcggatgtt cgtccgggtc gtcggggatc
    11761 cggggaacct caagtccgta tggaacaccg tggtgatcgc cctgtgggtc accgtactcg
    11821 ggaccgtcct gggcatcttc tacgcctggc tgatcggacg gagcgacatc cccctcaagg
    11881 ggctgatgaa atccctcttc accatcccct acatgttccc gcccttcttc ggcgccatgg
    11941 cctggagcct gttgctggcc ccccggtccg gatacatcaa ccggctcttc atggacctca
    12001 ccggggggac gacacccctg ttcaacatgc acagcatagc cgggatcgtc ttcgtggaga
    12061 cctgctatta cttccccttc gtcttcctgc aagtggtcag cgccctggag cgcatggacc
    12121 ccaccctgga ggaatcggcg cggatcgcgg gagcgggaca gtggtacgtg atccgcagga
    12181 tcaccctgcc cctggtcctt ccggccatag cggcgggggc catgctgatc ctcatctcct
    12241 ccctgtccca cttcggcgtc cccgccatcc tgggtttctc ccagaacatc ttcaccctgc
    12301 cgaccaagat ctatcaattg atccgccgct ccgcagggag cttccagggg atccgggagg
    12361 ccgcggccct ctccatcctg ctcatcgtcg tggtggtggc ggccctctac gtccagaaaa
    12421 gggtcctgaa gaccgggcgc tacgacatca tcaagggcaa gagcatgcgg cccatgctga
    12481 tcaagctgcg gggcgcgcgg ctgcccatcc tggccgtctg tctggcgtcg ctgggcgtga
    12541 tcgtggtcct gcccctggcg gtggtgatcc tggtgggatt cctcaaggcc tacggccttc
    12601 ccttccgctt cgagaacttc accatccgga attaccagta cgtcctcttc cggtcccgga
    12661 tggtgatcga ctccatccgc aacagcctgt tcctctcggt cgcctccggg gtcatcacca
    12721 tgttcctggg ggtgatggtc gcctacgtgg tggtcaaggt gaaaccccgg ggcaagggac
    12781 tcctggagat cctgtccctc ctgccttatt cgatgcccgg catcgtgctg gccatcggag
    12841 tcatcctggt ctggtcgggg gccttctacc tcaacctcta caacaccatc tggatcatcc
    12901 tgatcgccta catcgcgcgg tacgtttcct tcgccatgaa atccgcttcc gcgtccctgg
    12961 agcaggtcca ctactccctg gaggaggcgg cccggtcctg cggggcaacg cacctggagt
    13021 ccctgcggga cgtgaccctg cccctgatcc ggccggggat ggtggccggt ttcttcctga
    13081 tcttcctgcc ggggatgcgg gagctgacca cctcggtcct cctgtacggc cccttcaccc
    13141 ggaccctggg ggtggccatc tattccatga acgccgaggg caacacagta cagtcctccg
    13201 ccctggccac catcgccatc ggcatcatcc tggccggcaa cctggccctg cgctgggtca
    13261 ccaaagatcg gaagggggcc tgacatgagc cggattcagt tgaagggggt ctccaaggtc
    13321 ttcccgaccc ggagcggcgc ctccgtggcc gcggtcaagg atctcgacct cgtcatcgag
    13381 gagggggaat gcttctcctt tctgggcccc tcgggctgcg ggaagaccac gaccctccgg
    13441 atgatcgccg gcttcgagga cctcagcgcc ggggagatct ggctggggga ccgcgccgtc
    13501 tccatcaagg agcggaacct ctacataccc ccggaacagc ggggtctggg catggtcttc
    13561 caatccttcg ccgtgtggcc ccacctgaac gtcttcgaga acgtggcctt ccccctccgg
    13621 gtccgggacc ttccccggac ggacatcgcc gaggccgtgg cccaggccct gcgccacacg
    13681 gacctggcgg ggctggaggg gaccttcccc gcggacctga gcgggggcca gcagcagcgc
    13741 atcgccttgg cccgggccat cgtgaccaag ccccgggtca tgctcctgga cgagcccttg
    13801 tccaacctgg atcccaagct ccgggagtcc atgcgattcg agatcaagga gctccagcgg
    13861 aagctcaact tcaccatcgt cttcgtgacc cacgaccagg ccgaggccat ggccctttcg
    13921 gaccgcatgc tggtaatgga cctcgggaaa gtccagcagg cggacacgcc ggaggccctg
    13981 tacaaccggc ccgccagccg cttcgtctac ggcttcctgg ggctgtcgaa cttcacggac
    14041 gtggaactgc ggggcgggga cctgtacgtg ccggggtcga aatacccgct cccgatggaa
    14101 cttcccgccg gggccgcccg ggaaggcgtg atggccaccc ggcccaacga gatcaccctg
    14161 agcccgaagg acgaaggagg ctgcccggcc gtcgttgtga agcgcctgta cctgacctac
    14221 tgtatcgagt accaggcgcg ggtcggcgat cagaccctcc gggtgcattg cccccactcc
    14281 atcgtgttgg aggagggacg gccatgcttc ctgaggttta agaacccccg atggtatccc
    14341 cgggaatccg gccgggcgga gatcgagcgg aacgagagac aggtgatttg agtacttggg
    14401 gacgatccgg gagaggaacc aacggttccc ctcccggacc cttccttccc tacatcagcc
    14461 ccgggatgaa gagggccagg ggcggataca ggatgatcgc cagcaggacc agaaggatcc
    14521 cgagcagata cgggatcgcg ctgcgggcgg tcttgaggat cgagatcccg ccgatctcgc
    14581 tggctatgaa caggcaggcg cccacgggag gcgtcaccaa gccgatcgcc agggcgatga
    14641 cggtgacgat tccgaacagg atcggatcga tgcccatcat gcgggtcacg ggatatagaa
    14701 cgggcacgag caggatcatc gcggccccca ggtcgatgaa catccccgcc accaggtaca
    14761 cgaccacgaa gaggagaagc accacgttct tgtcctgggt cagcatcccg atcagggcgg
    14821 ccagcttctg tggaatctgg tcgttggtga tgatccagct gtagaggaag gccccggcga
    14881 tcatgaacat gacgatggcc gtggtgatcc cggactcgta gatcagcttg ggcaggtccc
    14941 gaggcttgag ctcccggtag acgaccatgc tgacgaacag ggcgtacacg atggccagca
    15001 cggcggcctc cgtgggagtc acgatcccga agatgattcc ccccaggatg atgaccggca
    15061 tgaaaagggc caggattccc cggcgcaggc tcccgaggaa atcccgtatg gagaaacgtt
    15121 tggggctgcc catcccgcgc ttggcgcaga tgaagtagga gatgaccatc tgcaccaggc
    15181 cgatcaggat gccggggacg atgcctccca ggaacaacct gccgatggag gtcccggtga
    15241 tcacgccgta gatgacgaag gggatgctcg gggggatgat g
//
LOCUS       DAOTEI010000076        15253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2662_length_15253_cov_5.354257, whole genome shotgun sequence.
ACCESSION   DAOTEI010000076 DAOTEI010000000
VERSION     DAOTEI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15253
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2662_length_15253_cov_5.354257"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="P5313_08805"
     CDS             complement(<1..880)
                     /locus_tag="P5313_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY80502.1"
                     /translation="MSATTAKKIDLEKHGALIALGALFVLASVIGWPYFIRPENLVNV
                     MRQSSYTGIIALGMTFVIISGGIDLSVGAVAAFVASCGVLFMNWVLQAAGGNEVLAIL
                     GGVSVMIALGFAAGALNGLLITKGKIVPFIVTLGTMSIFRYLSLQMANAGEFASQSQL
                     YGTFGMSKIFEFKVGDQAISLPIPVFTLLVLAAVLAFLLDNTRYGRYLCAVGSNARVA
                     QYSAVRIDRVRFWAYAINGMCVGTTAALLAARFNTVSTPNFGLGFEMDAIAATIIGGT
                     AMAGGRGSIWGTIIGVF"
     gene            complement(877..2388)
                     /locus_tag="P5313_08810"
     CDS             complement(877..2388)
                     /locus_tag="P5313_08810"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012069562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY80503.1"
                     /translation="MPDAILRVEKISKDFGSGPVLKDVDLEVRPGEILGIIGENGAGK
                     STLMKILAGIHQPTSGKVHVDGKAVRIADPIAARALKISLVPQEFNLIRDLPVYNNVF
                     LGAELRKRSGVLDKEAMKARTRELLAQLSVDLDPEARIEGLSAAEKQMVEICKALAFD
                     SRVLIMDEPTTMLTKYEIEILFRLVTKLRSEGMTILYISHKLKEVKHLCDRVVVLRDG
                     ELVHDGPTSDIGIVEMAQKMVGRELGSIFPAKGKPGDETVFEARGVTVPGVLEDISFQ
                     LRKGEILGLAGLVGAGRTELAETVLGLRHKSAGTFLKDGRELDLREPADAVAAGISYL
                     SEDRQGAGIITTFTVTRNVTLVSLGEYCRSFFRLIDKKTEKTKVQGYVDSFHIKTDTL
                     ERCLENLSGGNQQKVSLAKAIDTSPSVLIVDEPTRGVDVSAKHEIYVFINELARKGIS
                     CLLISSELEEIIGMCDRVLVMRDGRIAGTLEGDRITEEQIMYHATGVQEEAAS"
     gene            complement(2396..3385)
                     /locus_tag="P5313_08815"
     CDS             complement(2396..3385)
                     /locus_tag="P5313_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRY80504.1"
                     /translation="MSRPVTLVSGQWADLPFETFCQKAGAMGFDGVEIACWGDHMDVK
                     AAAADPRYVEDRKRILERNKLGCWALSAHLAGQCVGDPWDPRLDGFAPPQVKGNPDAI
                     VAWATEEMKLSARAAKNMGCKVVTCFMGSPIWRYWYSFPQTTEEMVENAYAEIVRRWT
                     PILDVFDECGVRFALEVHPTEIAFDYYTAGRLLAAFGRRDTLGFNFDPSHLLWQGVRP
                     DLFLRDFADRIYHVHMKDVAVRPDGRAGILGSHLPFGDLRRGWNFVSLGRGDVDFDAI
                     VRELNEAGYSGPLSVEWEDAGMDREHGAAESLAYVRRIDFAPSGRAFDAAMRK"
     gene            complement(3395..4396)
                     /locus_tag="P5313_08820"
     CDS             complement(3395..4396)
                     /locus_tag="P5313_08820"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRY80505.1"
                     /translation="MKTEALWYLGERKLELRPVDLPDPEANEVLVEMEACGICGWDLL
                     AYSGRFGKFHEYPFRAGHEGVGRIARTGSLVRDLAVGQRVVCHELPIGERGGGLMARH
                     AVRTREKVTPIPDEPFRPAELWLVEPVACVVNGIEYAGIRPGDSVALVGAGYMGLLML
                     QLLRRTLAGRVTVFEPDPRRLELARTFGRDMGGWDYVPDGSAVLQDRARSYDVVLETA
                     GIPATLELAFALAKPFAIIENFAWHHHRMDFDLDEWHTKGWRILNIQPQMNPSFDSLF
                     PRTVALMAAGAVTNEGLLTHTEPVENARAAYEAGLDRTGGYIKGAILFSAGSARTDR"
     gene            4658..5902
                     /locus_tag="P5313_08825"
     CDS             4658..5902
                     /locus_tag="P5313_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRY80506.1"
                     /translation="MTELDLDDPVNVSTTAGRIFKIIRDHGPLYGKDIVRRTGLAKST
                     VSAYVDRLMSVGLLREEIPDGMKRRKLKVAESAGYVVGVALGQSHLSVALCDLEAEII
                     DSVGGPVDLVRESPEKVLNRVISYARELEQRAGLGPSALFGIGFGLPSPVDYSHGVPV
                     NPPVMPGWDRFPVASFLSQEFTCPVFVDNDVNVMALAERDKGAASDLAYRKGSFLLVK
                     AGTGIGAGIVINGEIYRGAKGAAGDIGHIGIDGDGTLCRCGNTGCLEAVAGGRALAAR
                     AQAAARGDRSPYLAEALVRGEALDAEALARGAACGDEECLRLVASAGAYLGTVLAKLV
                     NFFNPSLIVISGGLTNLGERFIASVRESIYSRSTPLATADLIVKKSTLAGRGATMGAA
                     ILVLDEVFSHRNVGMLMRKISD"
     gene            complement(5909..6292)
                     /locus_tag="P5313_08830"
     CDS             complement(5909..6292)
                     /locus_tag="P5313_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80507.1"
                     /translation="MKTKEYIMHLLAEMSNYILDAKPMRMVISLHQEPDGLHLSVMDS
                     TVRKDSELEAITRALNTTARPEMSDYYGHMAGNDLVGSARLNLIGWQVKKADVERIEG
                     GTKIDLWLGSERFDSAKFSLPDGKS"
     gene            complement(6352..7608)
                     /locus_tag="P5313_08835"
     CDS             complement(6352..7608)
                     /locus_tag="P5313_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HRY80508.1"
                     /translation="MTEIEKSLYRLMLVLIAGTAAVGLFIEGPSEALREFLILQWSQS
                     RLIHDFSVTGGPGGALLNAALMAALSLLVVRMNGVRLSGPTIAGVLTVMGFSLFGKTA
                     LNVVPVVLGVWLAARSAGKTFKSYIMIALFGSALGPLTTTLALEIGLSGFGAVAAGIA
                     GGIAAGFILPGLAMAMLRLHEGYNLYNIGLTCGFFGLFAAALLAAARRDVPITVVWNG
                     APGPAMALLIPVFSVVLVGWGLALEGKGAISSFRKLMKLTGRLPSDFLDMASPGAALL
                     NMGVLGLIGSAYVALVGGSFNGPVLGGLFTLIGFGAFGKHPRNSLPVMAGVIASTLIF
                     GKSLTAPGPILALLFGTTLAPLAGEFGPLVGFAAGFLHLVLVERTGAWHMGMDLYNNG
                     FAGGLTAGLVAAAMEWIRSNKQERMK"
     gene            complement(7837..9495)
                     /locus_tag="P5313_08840"
     CDS             complement(7837..9495)
                     /locus_tag="P5313_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY80509.1"
                     /translation="MRIRTKMLALVLPLLVTPLFLSGLVSALEARNGITAVAVDFLRF
                     KAEELRKYAAGQWGILEENSLAEDPRYVSIAEDAVAGFAAGIIRSPTELVAAFDRGGS
                     LILSTSPLEMQESEARELRRLAAEGREGWERLRLGGVDRVAQAFVLPQFGWYVLVGER
                     EDRFYRAVDEIIRRSVYILAGSAVLSVILLVGFARYLTEPVVRLEKTMTRVMETSDLT
                     LRAEVRFSDEIGRLAESFNGMVEALDEAYARIKDYAAEAVHSRRRERKVRSIFQKYVP
                     ATVIDQIFERPDSALTGEERVLTVLFSDIVGFTGISESLKPAEVVDSLNHYFERMGGI
                     ILEQSGIVDKYIGDAIMGFFGAPSSTGREALDGVEAALRMQEALEEFNRWQVSKGRPP
                     FRSGVGLNHGTVTVGNIGFEKKMDYTAIGDMVNTASRIEGLTRYYREPLLVSSSVWRE
                     VRAKYPCRFVDKVEVKGRKGAVSVFAARREIGLSEEKAWKYFHAGIKRYYDRAFPEAL
                     RYFAAAARLAPGDRGVARFLSRCRDCIKNPPPPEWTGTTVMSEK"
     gene            9596..10849
                     /locus_tag="P5313_08845"
     CDS             9596..10849
                     /locus_tag="P5313_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80510.1"
                     /translation="MTIEPAAPSPDPSPPSAIPEEERQDTLREIEELIRAGHRPIRDG
                     KGQHPGTSGAVLPAIVIAASLVLMGAGFLILPGLYDRDEARMVLPAEAGPGGGAELFR
                     ALREEAGQQIEEREKAIGELRVQYDRARSERETLRREFDARLADRARELEAGIAAELE
                     AERRRLAAEGLTEAAVAARLKEFQDRQERERKAELDAFQARLAAEAAERDRQLAAAAA
                     RYNEELQRSTDPLKAEITRLTAERRGSEEARIQLERRYDEALAEAARARKDYEDRIAA
                     LERRLRDAEAQTARLSAAETRRTAARDRVDALLRTARGEPARTASRSGDQELLLDLLK
                     AKAEVRALLAEEPAKSARPGLAERLDRYFETFEGEYRSQGRKQAFSEAAEAAESAAAG
                     RPARGLPAGDADLVRFLEKLRDLLR"
     gene            complement(10897..11859)
                     /locus_tag="P5313_08850"
     CDS             complement(10897..11859)
                     /locus_tag="P5313_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80511.1"
                     /translation="MAGELDITPSFVTLEESKSVTLTVSGGRAPYSYLDISGDGILTP
                     LNGHAEYTAPALGADDSRLILITDSKGNQVEAEILVTVAPVPLLQVNPTVSSISTAGS
                     ATFTPSGGKTPYAYSVISGAGSVDPATGPSTTYTPSAVTTAVVRVTDSSGQIADATVY
                     VSAGPPPLVIDPPTLQIVTGGGFTFTATGGSPPYTFSATDGSIVPATGAYTAPGSPNP
                     SVTVTVTDSVAAAAAAAVTVIAPPAGDPLAIVPRALTIGKFKSFQFSAVDGSPPYQYF
                     IVAGNGSITLDGLYTASNKVGTETVKVRDSNGAEDFATITVKAK"
     gene            complement(11975..12916)
                     /locus_tag="P5313_08855"
     CDS             complement(11975..12916)
                     /locus_tag="P5313_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80512.1"
                     /translation="MEEILRVTIALAAEERGFRTIPDPAGVAATPQAEFLLSVRYSLT
                     GNRVTLRFGLNDTRSVLPESSGSLERTLDPQFDREVSLLVAEILLPAEREVAVRPRTV
                     PVSVPAVESPQAADATVQPGPSGAVTGIGEADGVRIPGRERRASRRPPRPDFGAGVYL
                     PLGTARSYFDTAGWFDARWGFPLGETGPWSAGPAAALAAFSMAGPDEAESGNYFMMLG
                     AELSFRVPGAGKVSPFASVSAGPALVVVRGADPERYAKLMPYAAARLGASLRVSPRWR
                     LGVDTTLLVLMDYEAGFESLSPVWGIAPRIFVSLESR"
     gene            complement(13121..14047)
                     /locus_tag="P5313_08860"
     CDS             complement(13121..14047)
                     /locus_tag="P5313_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY80513.1"
                     /translation="MTMRGTREAFIQGLLEAAQADPAVVLVCADSFKAARAAPFAEAF
                     PDRVFDVGIAEQAAAAFAAGLASCGYKPYVDTYAGFIAMRALEQVRLYAGYTGLNVKF
                     AGLNGGVYGGEREGVTHQFFEDLAALRAIPGMEIVVPADAGQVRQAVLALASRRGPAY
                     IRLGSGREPDILDPGERFEFGKLKVLSDTGTDVALFTQGPVLARVLKAADLLKARGVG
                     AVVVEAATLKPFDAEAAAAVASRCARIVTVEDHNILGGLGSAVAETLAERGLARPLVR
                     IGIRDVFPQSGPAEALLDHYGMRPEDIAAAAT"
     gene            complement(14044..14880)
                     /locus_tag="P5313_08865"
     CDS             complement(14044..14880)
                     /locus_tag="P5313_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY80514.1"
                     /translation="MLNQETRAALAAKARRLRRMVVTTVHQAGDGHPGPALSCADIVA
                     ALYYRCMRIDPARPRWEDRDRFILSKGHACTVLYAALADLGYFPESALPTFRSLGSIL
                     QGHPDMNKTPGVDMTSGSLGHGIPVGAGMAAAARLSGREFRVYTVVGDGELNEGIVWE
                     GALAAARMGLDKLVAFVDCNGFQSGGTTEEISGMSRIRERFEAFGWRCREIDGHDFDQ
                     IVDAVEEARAPTGKPTMILARTVKGKGVSFMENNNDWHKGVPDKDQLARALADLADGR
                     EP"
     gene            complement(14900..>15253)
                     /locus_tag="P5313_08870"
     CDS             complement(14900..>15253)
                     /locus_tag="P5313_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80515.1"
                     /translation="TERNYPAVMIGGGARALRHFTEMVGGALVVTINWVGTADKLIEE
                     DPDVVFRFFNPVPQSVLDELLLKLKDFRRGWEPDGLSVKEFEHFGPVVLFRDSFLQNW
                     NKVLEAVRERRPRLK"
BASE COUNT         2559 a         5184 c         5167 g         2343 t
ORIGIN      
        1 tgaagacccc gatgatggtg ccccagatgg agccccggcc cccggccatg gcggtgccgc
       61 cgatgatggt ggccgcgatg gcgtccatct cgaacccgag gccgaagttg ggggtggaca
      121 cggtgttgaa ccgggcggcc aggagggccg ccgtggtgcc cacgcacatc ccgttgatcg
      181 cgtaggccca gaaccggacc cggtcgatcc gcacagcgga atactgggcc acccgggcgt
      241 tggaccccac ggcgcagagg tagcggccgt accgggtgtt gtccaggagg aaggccagga
      301 cggcggccag gaccagcagg gtgaagaccg ggatcggcag ggagatcgcc tgatccccga
      361 ccttgaactc gaagatcttg gacatcccga aggtcccgta cagctggctc tgggaggcga
      421 actccccggc gttggccatc tggagggaca ggtaccggaa gatgctcatg gtgcccaggg
      481 tcacgatgaa ggggacgatc ttgcccttgg tgatcagaag gccgttcagg gctcccgccg
      541 cgaagcccag ggcgatcatg accgagacgc cccccaggat ggccaggacc tcgttgcccc
      601 ccgcggcctg caggacccag ttcatgaaga ggaccccgca ggaggccacg aaggcggcca
      661 cggcccccac ggacaggtcg atccccccgg agatgatgac gaaggtcatg cccagggcga
      721 tgatccccgt gtacgaggac tggcgcatca cgttgaccag gttctcgggg cggatgaagt
      781 agggccaccc gatgacggag gccaggacga agagggctcc cagggcgatc agggcgccgt
      841 gcttctccag gtcgatcttc ttggcggtcg tagcgctcac gatgcggcct cctcttggac
      901 tcccgtcgcg tggtacatga tctgttcttc cgtgatccgg tcgccctcca gggtccccgc
      961 gatccgtccg tcccgcatca ccaggacgcg gtcgcacatg ccgatgatct cctccagttc
     1021 cgaggagatg agcaggcagg aaatgccctt ccgggcaagt tcgttgatga acacgtagat
     1081 ctcgtgcttg gcggacacgt ccaccccccg ggtgggctcg tccacgatca gcaccgaggg
     1141 ggaggtatcg atggccttgg ccagggagac cttctgctgg ttcccccccg agagattctc
     1201 caggcaccgc tccagggtgt ccgtcttgat atggaaggag tccacgtaac cctggacctt
     1261 ggtcttttcc gtcttcttgt cgatcaatcg gaagaaggag cggcagtact cccccaggga
     1321 caccagggtc acgttgcggg tgaccgtgaa ggtggtgatg atcccggccc cctgccgatc
     1381 ctcggaaaga taggagatgc cggcggccac cgcgtccgcg ggttcccgga ggtcgagttc
     1441 ccggccgtcc ttcaggaagg tgccggcgct tttatggcgc agacccagga cggtctccgc
     1501 cagttcggtc cggcccgccc ccaccaggcc cgccagcccc aggatctcgc ccttccgcaa
     1561 ctggaaggag atatcctcca ggaccccggg caccgtgacg ccccgggcct cgaagaccgt
     1621 ctcgtccccg ggctttccct tggccgggaa gatggaaccc agctcccggc ccaccatctt
     1681 ctgggccatc tccacgatgc cgatgtccga ggtcgggccg tcgtggacca gttccccgtc
     1741 ccgcaggacc accacccgat cacagaggtg cttgacctcc ttgagcttgt gggagatgta
     1801 caggatagtc atgccctcgg accggagctt cgtcaccagc cggaacaata tctcgatctc
     1861 gtacttggtc agcatggtgg tgggctcgtc catgatcagg acccgggagt cgaaggccag
     1921 ggccttgcag atctccacca tctgcttttc cgccgcggac aggccctcga tccgggcctc
     1981 gggatccagg tccacgctga gctgggccag gagctcccgg gtccgggcct tcatcgcctc
     2041 cttgtccagg accccggagc gcttccggag ctccgcgccc aggaagacgt tgttgtacac
     2101 cgggaggtcc cggatcaggt tgaactcctg gggcaccagg gagatcttga gggcccgggc
     2161 ggcgatgggg tccgcgatcc ggaccgcctt cccgtccacg tggacctttc cggaagtggg
     2221 ctggtggatc cccgccagga tcttcatcag ggtgctcttg cccgccccgt tctccccgat
     2281 gatccccagg atctccccgg ggcggacctc gagatccacg tccttgagga cgggtccgct
     2341 cccgaagtcc ttggagatct tctctacccg aaggatggcg tccggcatcg gcccctcact
     2401 tcctcatggc cgcgtcgaag gcgcggcccg acggcgcgaa gtcgatgcgg cggacgtagg
     2461 ccagggactc cgcggccccg tgctcccggt ccatcccggc gtcctcccac tccacggaca
     2521 ggggccccga gtatccggct tcgttcaact cccggacgat ggcgtcgaag tccacgtccc
     2581 cccggcccag ggacacgaag ttccaacccc tccgcaggtc cccgaagggc aggtgggacc
     2641 ccaggattcc cgcccggccg tcgggccgca ccgccacgtc cttcatgtgg acgtggtaga
     2701 tccggtccgc gaagtcccgg aggaacaaat ccggccggac gccctgccac aggaggtggg
     2761 aggggtcgaa attgaagccc agggtgtccc ggcgcccgaa ggcggccagg agccgcccgg
     2821 cggtgtagta gtcgaaggcg atctccgtgg ggtggacctc cagggcgaag cgcaccccgc
     2881 actcgtcgaa gacgtccagg atgggggtcc agcgccggac gatctccgcg taggcgttct
     2941 cgaccatctc ctcggtggtc tgggggaagg agtaccagta gcgccagatc ggggagccca
     3001 tgaagcaggt caccaccttg cagcccatgt tcttggccgc ccgggcgctc agcttcatct
     3061 cctccgtcgc ccaggcgacg atggcgtcgg gatttccctt gacctggggc ggggcgaagc
     3121 cgtccaggcg gggatcccag gggtccccca cgcactgacc ggccaggtgg gccgagaggg
     3181 cccagcagcc gagcttgttg cgttccagga tccgcttccg gtcctccacg taccggggat
     3241 ccgccgcggc ggccttgacg tccatgtggt cgccccagca ggcgatctcc accccgtcga
     3301 agcccatcgc tccggccttt tggcagaagg tctcgaaggg aaggtcggcc cactgaccgg
     3361 ataccagggt tacgggtcgg ctcatggttc ctccttaacg gtccgtccgg gcggagcccg
     3421 cggagaagag gatcgctccc ttgatgtagc cccccgtccg gtccagcccc gcctcgtagg
     3481 ccgcccgggc gttctccacg ggctccgtgt gggtcaacag gccctcgttc gtgaccgccc
     3541 cggcggccat caaggcgacg gtccggggga atagggaatc gaaggaagga ttcatctggg
     3601 gctggatgtt gaggatgcgc cagcccttgg tatgccactc gtccaggtcg aagtccatgc
     3661 ggtgatggtg ccaggcgaag ttctcgatga tcgcgaaggg cttggccagg gcgaaggcca
     3721 gctccagggt cgccgggatg cccgcggtct ccaggaccac gtcgtaggat cgggcccggt
     3781 cctgcaggac cgcggacccg tcgggaacgt agtcccagcc gcccatgtcc cgcccgaagg
     3841 tccgggccag ctccagacgc cggggatccg gctcgaagac cgtcacccgg cccgccaggg
     3901 tccttcgcag gagctggagc atcaggagtc ccatgtatcc cgccccgacc agggccaccg
     3961 agtcgccggg ccggatgccc gcgtattcta ttccgttcac cacacaggcc acgggttcca
     4021 cgagccagag ctcggcgggc cggaaaggct cgtccgggat cggtgtgact ttttcccggg
     4081 tccggacggc gtgccgggcc atgaggcccc cgccccgctc tccgatgggc agctcgtggc
     4141 agaccacccg ctggccgacg gcgaggtccc ggaccaggga gccggtccgg gcgatccggc
     4201 cgacgccctc gtggccggcc cggaaggggt actcgtggaa tttcccgaag cggccggaat
     4261 aggccaggag gtcccagccg cagatcccgc aggcttccat ctccacgagg acttcgtttg
     4321 cttcggggtc gggcaggtcg acgggacgga gttccagctt ccgttcgccg aggtaccaaa
     4381 gggcttccgt tttcatgatg ggctccggac ccagcctaca accggttctc cgatctgtca
     4441 acggaattgt tcgattgtcg aacttttttt gcatccgtcg aactttttcc gttgacaggt
     4501 gaaccgtccg ggcggatact tccccatacc ctcgtggtac gaagcttgtg gccccggatg
     4561 cggcgtcccc cgcgccgtcc gaccgcttga cggggccggc cttcgcggcg atcatcccgg
     4621 atcatccgga tcgccgtgcc gacgaaaggc gtgcatcatg accgagctcg atttggacga
     4681 ccccgtcaac gtctccacca ccgcgggcag gatcttcaag atcatccggg accacggccc
     4741 cctgtacggc aaggatatcg tccgccgcac gggcctggcc aagtccacgg tctccgcgta
     4801 cgtcgatcgg ctcatgtccg tgggcctcct gcgggaggag atccccgacg gcatgaaacg
     4861 ccgcaagctc aaggtggccg agtccgcggg ctacgtggtc ggggtggccc tggggcaatc
     4921 ccacctttcc gtggccctct gcgacctgga ggcggagatc atcgactccg tgggggggcc
     4981 cgtcgacctg gtccgggaaa gcccggaaaa ggtcctgaac cgggtgatct cctacgcccg
     5041 ggagctggaa cagcgggccg gactcggtcc ctcggccctc ttcggcatcg gcttcgggct
     5101 tcccagtccc gtggactact cccacggagt cccggtgaac cccccggtca tgccgggctg
     5161 ggaccgtttt cccgtggcgt ccttcctctc ccaggaattc acctgtccgg tcttcgtgga
     5221 caacgacgtc aacgtcatgg ccctggcgga gcgggacaag ggggcggcct cggacctggc
     5281 ctaccggaag gggagcttcc tcctggtcaa ggccggcacg ggcatcgggg cgggcatcgt
     5341 catcaacggg gagatctacc ggggagccaa gggtgccgcg ggggacatcg ggcacatcgg
     5401 catcgacggc gacgggacac tgtgccgctg cggcaacacc gggtgcctgg aggccgtggc
     5461 gggcggtcgg gccctggccg cccgggccca ggccgcagcc cggggggacc gcagccccta
     5521 cctggcggag gccctggtcc ggggggaggc tttggacgcg gaggctctgg cccggggggc
     5581 cgcctgcggc gacgaggaat gcctgcgctt ggtggcctcc gcgggggcct acctgggcac
     5641 ggtcctggcc aagctggtca acttcttcaa cccctccctg atcgtgatct ccggagggct
     5701 caccaacctg ggggagcgct tcatcgcctc cgtacgggag tccatctaca gccgatccac
     5761 gcccttggcc accgcggacc tgatcgtcaa gaaatcgacc ctggcaggac ggggcgccac
     5821 catgggggcc gccatcctgg tcctggacga ggtcttctcc caccggaacg tggggatgct
     5881 gatgaggaag atctcggact gaggaccgct agctcttccc gtcgggcagg gagaacttcg
     5941 cggagtcgaa gcgctcgctg cccagccaga ggtcgatctt cgtgcccccc tcgatccgct
     6001 ccacgtccgc cttcttgacc tgccagccga tcaggttgag ccgggcggac cccaccaggt
     6061 cgtttccggc catatggccg taatagtccg acatttccgg ccgggcggtc gtattgaggg
     6121 cccgcgtgat ggcctccagt tcggagtcct tgcgcaccgt ggagtccatg acggacaggt
     6181 gcaggccgtc gggttcctgg tgcagggaga tcaccatgcg catgggcttg gcgtccagga
     6241 tatagttgga catctccgcc aggagatgca tgatgtattc cttcgtcttc atggatggcc
     6301 gcctttcgtg ggcaccgcgg agcgggggaa cccggaccgc gccccggcat tttacttcat
     6361 ccgctcctgt ttgttcgagc ggatccactc catcgccgcg gctaccaacc ccgcggtgag
     6421 tcctccggcg aagccgttgt tgtacagatc catgcccatg tgccaggccc cggtgcgctc
     6481 caccaggacc aggtgcagga agcccgccgc gaatcccacc agggggccga actccccggc
     6541 caggggggcc agggtcgtgc cgaacaggag ggccaggatg ggtcccgggg cggtcaggga
     6601 ctttccgaag atcagggtgg acgctatgac tcccgccatg acgggcaggg agttccgggg
     6661 atgcttgccg aaggccccga acccgatcag ggtgaacagg ccccccagga cggggccgtt
     6721 gaagctcccc cccaccaggg ccacgtaggc gcttccgatc aggcccagga cgcccatgtt
     6781 gagcagggcc gccccggggg aggccatgtc caggaagtcc gagggcagac ggccggtcag
     6841 cttcatgagc ttccggaagc tcgagatcgc tcccttcccc tccagggcca gtccccagcc
     6901 gaccaggacc acggaaaaga cggggatcag cagggccatg gccggtcccg gggccccgtt
     6961 ccagaccacc gtgatgggta cgtcccgccg ggcggcggcc aggagggcgg ccgcgaagag
     7021 cccgaagaac ccgcaggtga gcccgatatt gtagagattg tatccctcgt ggaggcgcag
     7081 catggccatg gccaggcccg gcaggatgaa gcccgcggcg ataccgcccg cgatgcccgc
     7141 ggccacggcg ccgaatccgg aaagtccgat ctccagggcc agggtggtcg tcaggggccc
     7201 cagggccgag ccgaacaggg cgatcatgat gtagctcttg aaggtcttgc ccgccgaacg
     7261 ggcggccagc cagaccccga ggaccacggg aacgacgttc agggcggtct tcccgaagag
     7321 gctgaagccc atgaccgtca ggacgccggc gatggtcggc cccgagaggc ggactccgtt
     7381 catccggacc accagcaggg acagggcggc catgagggcg gcgttgagca gggcccctcc
     7441 gggtcccccg gtcacggaaa agtcatggat cagccgggac tggctccact ggaggatcag
     7501 gaactcccgg agggcctccg agggcccctc tatgaacagt ccgaccgcag cggtccccgc
     7561 gatcaggacc agcatcagcc ggtagaggga tttctcgatt tccgtcatcg cacctccatg
     7621 aatacggcca tcctcccgga ccacgggaac gacgggggag aacctgggga tcggcacgcg
     7681 acgccgacca tatcggggag actcccgcgt gaaaccgggg ccgtccggga cgaaccgacc
     7741 cggcacgacc cgtgaagcct gacgcctcgc gggggatgca taattatacg gtataattcc
     7801 ggtcgggaca agagaaaatt tttcccgatc cgggtctcac ttctccgaca tcaccgtcgt
     7861 gccggtccac tcggggggcg ggggattctt gatgcagtcc cgacagcggg acaggaagcg
     7921 ggcgacgccc cgatccccgg gggccaggcg cgcggcggcc gcaaagtacc ggagcgcctc
     7981 cgggaaggcc cggtcgtaat accgcttgat ccccgcgtgg aagtacttcc aggccttctc
     8041 ctcggaaagc ccgatctccc ggcgggcggc gaacaccgag accgcccctt tgcggccctt
     8101 cacctccacc ttgtccacga aacggcaggg gtacttggcg cgtacctccc gccagaccga
     8161 ggaggaaacg agcagaggtt cccggtagta ccgggtcagg ccctcgatcc gggaggcggt
     8221 gttcaccatg tccccgatgg cggtataatc cattttcttc tcgaagccga tgttgcccac
     8281 cgtcacggtg ccgtggttga gccccacccc gctgcggaag ggcggccggc ccttggagac
     8341 ctgccagcgg ttgaactcct ccagggcttc ctgcatccgc agggccgcct ccaccccgtc
     8401 cagggcctcc cggcccgtgg acgagggggc tccgaagaac cccatgatgg cgtcgccgat
     8461 gtacttgtcc acgataccgg actgctccag gatgatcccg cccatgcgct cgaagtagtg
     8521 gttcagggaa tccaccacct ccgcgggctt gaggctctcg gagatccccg tgaatcccac
     8581 gatgtccgag aagaggacgg tcagtacccg ctcctccccg gtcagggcgg agtccggacg
     8641 ctcgaagatc tggtcgatca ccgtggcggg cacgtacttc tggaagatgc tccgcacctt
     8701 gcgctcccgg cggcgggagt ggacggcctc ggcggcgtag tccttgatcc gggcgtaggc
     8761 ctcgtccagg gcctcgacca tgccgttgaa gctctccgcc aagcggccga tctcgtcgga
     8821 gaagcggacc tcggcgcgta gggtgaggtc gctggtctcc atgacccggg tcatggtctt
     8881 ctccagacgg accaccggct ccgtcaggta ccgggcgaac ccgaccagga ggatcacgga
     8941 gaggacggcg gaacccgcca ggatgtagac ggaacggcgg atgatctcgt ccaccgcgcg
     9001 gtagaaccgg tcctcccgct cgcctaccag gacgtaccag ccgaactgtg gcaggacgaa
     9061 ggcctgggcc acccggtcca ccccgcccag ccggaggcgc tcccagccct cccggccctc
     9121 agcggccagt cggcggagtt cccgggcctc ggattcctgc atctccagcg gcgaggtgga
     9181 aaggatcagg cttccccccc ggtcgaaggc cgcgaccagc tccgtggggc tccggatgat
     9241 ccccgcggcg aagccggcca cggcgtcctc ggcgatcgag acgtaccggg gatcctcggc
     9301 cagggagttt tcctccagga tgccccactg cccggcggcg tacttgcgga gctcctcggc
     9361 cttgaaccgg aggaagtcca cggccacggc ggtgatgccg ttccgggcct ccagggccga
     9421 caccagcccc gagaggaaga ggggggtcac caggagaggc aggaccaggg ccaacatttt
     9481 cgttctgatt ctcatattta tgtatagaat aagcccggag gcctccaaaa gcaaccgcgc
     9541 gaatttgaac tgagccggga tcggcagtac acttgaggca acaaggaaaa agtagatgac
     9601 catcgaaccc gccgcaccct cgccggatcc ctccccgcct tccgctattc ccgaggagga
     9661 gcgccaggac accctccggg agatcgaaga gctgatccga gccgggcacc gtcccatcag
     9721 ggacggcaaa ggacagcacc cggggaccag cggcgcagtc ctgcccgcga tcgtgatcgc
     9781 cgcttccctg gtcctgatgg gggcgggatt cctcatcctg cccgggctct acgaccggga
     9841 cgaggcccgg atggtcctcc ccgccgaggc gggtcccggc gggggcgcgg aactcttccg
     9901 tgccctccgg gaggaggcgg gtcagcagat cgaggagcgg gaaaaggcca tcggggagct
     9961 tcgcgtccag tacgatcggg cccggtcgga gcgggaaacc ctgcggcggg agttcgacgc
    10021 ccgcctggcc gaccgcgccc gggaactgga agccggcatc gcggcggagc tggaggccga
    10081 gcggcggagg ctggccgcgg agggcctgac cgaggccgcc gtggcggccc ggctcaagga
    10141 gttccaggat cggcaggagc gggaacggaa ggcggagctg gatgccttcc aggcccggct
    10201 ggccgcggag gcggcggagc gggaccgaca gcttgccgcc gcagcggccc gttacaacga
    10261 ggagctccaa cgcagcacgg accccctgaa ggcggagatc acgcggctga ccgcggaacg
    10321 gcggggcagc gaggaggccc ggatccaact ggagcggcgc tacgacgaag ccctggcgga
    10381 ggccgcccgg gcgcgcaagg actacgagga ccggatcgcc gccctggaac gtaggcttcg
    10441 ggacgcggag gcccagacgg cgcggctttc cgcggcggaa acccgcagga ccgcggctcg
    10501 ggaccgtgtc gacgccctgc tccggaccgc ccggggggag cccgcccgga ccgcctcccg
    10561 gtccggggac caggaactcc tcctggacct cctcaaggcc aaggccgagg tccgggccct
    10621 cctggccgag gagcccgcca agtccgcacg tcccggcctg gcggagcgcc tggaccgcta
    10681 cttcgagacc ttcgaggggg aataccggag ccagggccgg aagcaggcct tctccgaggc
    10741 cgccgaagcc gcggaaagcg ccgcagcggg ccgccccgcc cggggcctgc ccgccggaga
    10801 cgcggacctg gtccgcttcc tggagaaact gagggacctg ctgaggtagg aatcgcggga
    10861 acgggctcgg ccggtccgcg gggcgccggg tccgggctac ttcgccttga ccgtgatggt
    10921 cgcgaagtct tcggccccgt tggagtccct cacctttacc gtttccgtgc ccaccttgtt
    10981 ggacgcggtg tacagcccat ccaaggttat cgatccatta cccgcgacta tgaaatattg
    11041 atacggaggg ctcccgtcca ccgcgctgaa ttggaagctc ttgaacttgc cgatggtcag
    11101 ggcccggggg acgatggcga ggggatcgcc tgcgggcggc gcgatgacgg ttactgcggc
    11161 ggccgccgcg gccgcgacgg aatccgtgac cgtcaccgtc acgctcggat tgggagagcc
    11221 gggggcggtg tacgccccgg tcgcgggaac gatcgagccg tccgtagcgc tgaaggtgta
    11281 cggcggggac cctcccgtgg ccgtgaacgt gaagcccccc cccgtcacga tctgcagggt
    11341 cgggggatcg attaccaggg gcgggggacc ggcggagacg tagaccgtgg cgtccgcaat
    11401 ttgaccggag gagtcggtga cccggaccac cgcggtggtg acggcggagg gtgtgtacgt
    11461 cgtgctcggg cccgtggccg gatcgacgga cccggctccg gatatcacgg aatacgcata
    11521 gggtgtcttg cccccggaag gagtgaacgt ggcagagccc gccgtcgaga tggagcttac
    11581 ggtcgggtta acctgcagca ggggtaccgg cgcgacggtc accaggatct ccgcttcgac
    11641 ctgatttccc ttggaatcgg ttatcaggat caaacggcta tcgtccgccc cgagggctgg
    11701 agccgtgtac tccgcatgtc cgttcagggg agtgagaatc ccgtccccgg aaatatccag
    11761 ataggaatag ggcgccctcc cgcccgaaac ggtcagggtc acgctcttgg attcctccag
    11821 ggtcacgaag gacggagtga tatccaactc cccggccata aaatcccccc agacctcgta
    11881 gagggggggg gagttcgcag gaaaatgcgg ccaggagaag gacgaccccg gccgacaggg
    11941 cagcgaagga ccggaacgga tttttcatgg cctcctacct cgactccaag gaaacgaaga
    12001 tacggggcgc aatcccccag acgggggaaa gcgactcgaa cccggcctcg tagtccataa
    12061 ggaccaaaag ggtcgtatcc acgccgagcc tccaccgagg cgaaacccgg agggaagccc
    12121 cgagccgcgc agccgcatag ggcatgagtt tggcgtaccg ctcgggatcc gctccccgga
    12181 ccaccacaag ggcggggccc gcggagacgg aggcgaaggg agacaccttg cccgccccgg
    12241 gaacccggaa gctcaactca gcgcccaaca tcatgaaata gttgccggat tccgcttcgt
    12301 cgggtccggc catggaaaat gcggctaacg ccgccgcggg ccccgcgctc cagggcccgg
    12361 tttcgcccag gggaaagccc caccgggcgt cgaaccatcc cgcggtatcg aagtagcttc
    12421 gggcggtccc caagggcagg tatacccccg cgccgaagtc cggccggggc ggccgtcggg
    12481 acgcccgccg ttcgcgtccg ggtatcctga ccccgtcggc ctctccgatc ccggtcacgg
    12541 cgccggaggg tcccggctgt accgtcgcat ccgcggcctg cggcgactcc accgcaggca
    12601 cggagaccgg aaccgtacgc ggccggacgg cgacctcccg ctccgcgggg agcaggatct
    12661 cggcgaccag gagcgaaacc tcccggtcga actggggatc cagggtccgc tccaggcttc
    12721 ccgaggattc gggaaggacc gaccgggtat cgttcagccc gaatcgaagg gtcacccggt
    12781 tcccggttag agaatatcgc accgaaagca ggaattccgc ttgtggggtc gcagcgacac
    12841 cggccggatc gggaatggtc cggaatcctc tctcctccgc ggccagggcg atcgtgaccc
    12901 ggaggatttc ctccaagacc ggggatactt ccgaggaccc ggaattctcg ggacgtatcg
    12961 aaacggctat gacgggagcc tgcgcgtaca cgagtgccgg aaccgcgccc gcgaggatga
    13021 aagcggcgaa agcggcggac aggcgaattc tcagtctggc ctcccggtta tcccctcccc
    13081 caaaggataa ctataaccgt tcctgagtga tatgtaaaat ttatgtcgcg gcggccgcga
    13141 tgtcctccgg gcgcatgccg tagtggtcca ggagggcctc cgcggggccg gactggggga
    13201 acacgtcccg tatgccgatc cggacgaggg gccgggccag gccccgctcc gccagggtct
    13261 ccgccaccgc ggagcccagg cctcccagga tgttgtggtc ctccacggtc acgatccggg
    13321 cgcagcggga ggcgacggcc gcggctgcct cggcgtcgaa gggcttgagg gtggcggcct
    13381 cgacgaccac cgcgccgacg ccccgggcct tgaggaggtc cgcggccttt agcacccggg
    13441 ccaggaccgg cccctgggtg aagagggcca cgtcggtccc cgtgtccgag agcaccttga
    13501 gcttcccgaa ctcgaagcgc tcgccgggat ccaggatgtc cggctcccgg ccgctgccca
    13561 ggcggatgta cgcgggcccg cgccgggaag ccagggccag gaccgcctgg cgaacctggc
    13621 ccgcgtccgc gggcaccacg atctccatgc ccgggatggc ccgcagggcg gccaggtcct
    13681 cgaagaactg gtgggtcacc ccctcccgct ctccgccgta gacgcccccg ttgaggcccg
    13741 cgaacttgac gtttaggccc gtatacccag cgtagagccg gacctgctcc aaggcccgca
    13801 tggcgatgaa gcccgcgtag gtgtccacgt agggcttgta cccgcaggag gccaggcccg
    13861 cggcgaaggc cgccgcggcc tgctcggcga tgcccacgtc gaagacccgg tccgggaagg
    13921 cttcggcgaa gggggccgcc cgggcggcct tgaaggagtc cgcgcagacc aggacgacag
    13981 ccggatccgc ctgggccgcc tccaggagac cctggatgaa ggcttcccgg gttcctcgca
    14041 tcgtcatggc tccctcccgt cggccaggtc cgccagggcc cgagccagct ggtccttgtc
    14101 cgggaccccc ttgtgccagt cgttgttgtt ctccatgaaa gagacgccct tgcccttgac
    14161 cgtgcgggcc aggatcatcg taggcttgcc ggtcggcgcc cgggcctcct ccacggcgtc
    14221 cacgatctgg tcgaagtcgt ggccgtcgat ctcccggcat cgccagccga aggcctcgaa
    14281 gcgctcccgg atgcggctca tcccgctgat ttcctccgtg gtgcccccgc tctggaaacc
    14341 gttgcaatcc acgaaggcaa ccagtttgtc caggcccatc cgggccgccg ccagggcgcc
    14401 ctcccagacg atgccctcgt tgagttcccc gtcccccacg acggtgtaga cacggaattc
    14461 ccggcccgag agccgggccg ccgcggccat gccggcgccc acggggatgc cgtggcccag
    14521 ggagccgctg gtcatgtcca ccccgggggt cttgttcatg tccgggtggc cctggaggat
    14581 ggagcccagg gaacggaagg tcggcagggc ggactcgggg aagtacccca ggtccgctag
    14641 ggcggcgtag aggaccgtgc aggcgtggcc cttggacagg atgaagcggt cccggtcttc
    14701 ccaccgaggc cgggccggat cgatccgcat gcaccggtag tacagggcgg ccacgatgtc
    14761 cgcgcaggac agggccgggc cgggatggcc gtctcccgcc tggtggacgg tggtcaccac
    14821 catcctccgg agtctccggg ccttggccgc cagggcggcg cgggtctcct ggttcagcac
    14881 ggttccctcc tcgacggggt cacttgagcc ggggacgacg ctcccggacg gcctccagga
    14941 ccttgttcca gttctgcagg aaggaatccc ggaagaggac caccggcccg aagtgctcga
    15001 actccttcac ggacagcccg tcgggctccc agccccggcg gaaatccttg agcttcaaga
    15061 gcagttcatc caggacggat tggggaacgg ggttgaagaa gcggaagacc acgtccgggt
    15121 cctcctcgat cagcttgtcc gcggtcccca cccagttgat ggtcaccacc agggcgcccc
    15181 ccaccatctc ggtgaagtgc cgcagggccc gggccccgcc cccgatcatg accgccggat
    15241 agttccgctc cgt
//
LOCUS       DAOTEI010000077        15084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2707_length_15084_cov_5.207466, whole genome shotgun sequence.
ACCESSION   DAOTEI010000077 DAOTEI010000000
VERSION     DAOTEI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_15084_cov_5.207466"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="P5313_08875"
     CDS             complement(<1..202)
                     /locus_tag="P5313_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRY80516.1"
                     /translation="MDSSVTMEKIVSLAKRRGFVFQSSEIYGGQAGAWDYGPAGIELK
                     NRIQRFWWKEMTQLHDDIVGLDA"
     gene            complement(467..1801)
                     /locus_tag="P5313_08880"
     CDS             complement(467..1801)
                     /locus_tag="P5313_08880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRY80517.1"
                     /translation="MADFKVKVACRIYGFRHRKTFSASRPLEGNGPFMDADEKYWWGY
                     KYYWGPIEEPEPGAGLEEHFRGKDLDFRPRDGFEEAAGFTLCVGGDMMPYPGIRPETT
                     ESLWDDVRPFFFSGDLVCANLESPVAPSLPARYIPESILLPGLLNGTPEMFDRIVDGG
                     RGVTFFSTANNHCLDMGEAGLRETLDFLDARGYRHVGTARSTEERDAVEIAERNGVKV
                     AFLSFTFGLNWGKLPKGREYLANYVRFNKPNEDLSPVARQVRAARAAGADAVVALIHW
                     GLEFESFPVRSQVETAHRLIELGIDTVVGNHPHNVQPAERHTYTDPEGVRRDGLILYA
                     LGDLLSIHRTLPNSRLACLARIRISKGSVSGKSATRVTSLETLPVYLHVGRRGRQVVD
                     YRVLDFRRLADSLRAGDDRYGLGTRKTREVFRLEALLHRILGPALGAAGSAP"
     gene            complement(1873..2592)
                     /locus_tag="P5313_08885"
     CDS             complement(1873..2592)
                     /locus_tag="P5313_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80518.1"
                     /translation="METREQYFTRINRFGRISFAIFFVLMFAIPTVVCAVYDIFPSLA
                     TALKATMGLFLILTPIALSEIVSFVPLLGSGASYLTFETGNVSNIKLPCAMNALKLAG
                     VQPASEEGEVLSTISVAISSIVTTAVIMAAALLLAPLAPVFANPSVRIATANVLPALF
                     GALVVAFLTGGDSGPVVIRGQWKAFILPLLVVAVIHYLVFPIKGWEGVTILVLLPITI
                     LGARFLFKRGQITVVSKEAKP"
     gene            complement(2603..3319)
                     /locus_tag="P5313_08890"
     CDS             complement(2603..3319)
                     /locus_tag="P5313_08890"
                     /inference="COORDINATES: protein motif:HMM:NF027796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HRY80519.1"
                     /translation="MNFFDVANGSLLFVLVGAGIAYVLVQCVLFLRMSLKRARELGID
                     PKVVSNTIKSSAVFTLAPSIAVALGLAAMAASLGIAWPWFRLSVIGSVSYELMAANMA
                     SSALGFAKLADASKAGPEPLGAIMYAMTGGLAGAIILDILFIKKVDSLAVKMKAKAGS
                     FGIVAIGVLYYAVLAVFVAPFFGQGVVAVATFFTSIVLTLFQGFLARKFKIVWLGNFI
                     LSFSLILGMCSAVLWTALAK"
     gene            complement(3363..4817)
                     /locus_tag="P5313_08895"
     CDS             complement(3363..4817)
                     /locus_tag="P5313_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRY80520.1"
                     /translation="MEGKRVVLESVDRNRALYEALSDRIWGFAETGFQERASAAAIAD
                     RLESEGFTVDRGIAGMETAFVGSYGSGSPVVAVLGEYDALPGLSQAASLPEQKELDSG
                     KAGHGCGHNLLGIGALAAAVAAKDFLRDSGVPGTVRYYGCPGEEFGCGKTFLVRAGVF
                     GDVDLAFCWHPSDINAVWQARTLANLSVYFRFRGKASHAAASPHLGRSALDAVELTNV
                     GANYLREHIVPEARLHYAITNPGGRAPNVVQASAESFYYCRAPRIAQAREIYERLCDV
                     ARGAALMTGTNLEIVFAQGLSDYVPNLVLGEHLQKCMEEAGGPAFDAADLELAEKFRA
                     TLTEAEKAAILGQLRMTQGPAVLEAIGGSPLDSVVGPLFRGDICLPGSTDVGDVSQVV
                     PTGQIVYAAAALGTQAHSWQNTAQAGSSIGRKGMLAAGRALGLAAAAAFLDPALVERA
                     REEHASKVGDSYSSPIPETIEPEDVLRSVSNVPF"
     gene            5012..5686
                     /locus_tag="P5313_08900"
     CDS             5012..5686
                     /locus_tag="P5313_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY80521.1"
                     /translation="MAYRKSGETRERILKAAARLFSERGYYETGIGDIAREAGIGRAS
                     FYYHFTDKEKAARAVFDTYVEGIYAAAERVVPKSDANASAEELRRPLLLSVFVEYILL
                     FRFIALNKATHAVYYDLVNFADYDRENIDRLKRTTFKDTVFLASLYGRRLTEAELVAF
                     IVTTNAAAKSIFKAVSTGILDFDLYEASDYFFRHAVLPDIPIPEPEYRNLLIQAFSLC
                     ESVDLS"
     gene            5760..6527
                     /locus_tag="P5313_08905"
     CDS             5760..6527
                     /locus_tag="P5313_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY80522.1"
                     /translation="MRDATVLLGGRPVLREASFTLEEDEHVCILGPNGSGKSTIVRLI
                     SGDLFPLYREPAAVRLFGRERWDLFQIRGRLGIVSDTLQAWQAAGGESVRDTILSGFF
                     GGVGLPMRGAPETWRERKAEEAARILGIEELLGRLVGTLSSGQMRRVLVARALVHEPK
                     TLLLDEPYTSLDIAARHTFAERVRHLAARGHAIILVTHELSEIPPEMDRVILVKEGRI
                     LADGPKREVLVSGVISELFGIPLTVREENGRYRAVPA"
     gene            6570..7001
                     /locus_tag="P5313_08910"
     CDS             6570..7001
                     /locus_tag="P5313_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRY80523.1"
                     /translation="MILEARTARLVKSQDLNHHQTLFAGRAAEWFTETCFIAAARFTG
                     DPAGLVCVRIHGLTFTKPAHPGDCVEIVARIAHTGRTSVTVSGEIFINETPTPTVKGF
                     ATFVHVDADGKALPHGLSLPADYLARHRSLHEEALSLPRQG"
     gene            complement(7026..8552)
                     /locus_tag="P5313_08915"
     CDS             complement(7026..8552)
                     /locus_tag="P5313_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HRY80524.1"
                     /translation="MEHNPETVGKEPGLTESKTAYNPPKGARASLSIRIDGRELPYQA
                     EADWIILRKDEKPAAEMFYTGYFLPDGRDRPVTFVFNGGPGSSSVYLHFGALGPRRAV
                     FGPRGEPEPPPHKLEDNQETWLRFTDLVFIDPIGTGLSRMIPDAEAKKDPEKKGSDEK
                     KDVEYWGLKRDLESICEFIRKFLSRKKRWDSPVYLAGESYGGFRVAKLVRLLQQDYGV
                     ALAGAVLISPALEFALLDPSDYDDLPWVDTFPTMAGAACFHDRARKWKPGESRQDYAR
                     RASEFAVTELLPVLAAGDLVGEARRVRVLDAAADFIGLPRDIVRSKGGRVSIEYFAKS
                     LLRDRGLVLGLYDASATVRDPYPDRDDWSGPDPTLHVVERIFTAGVNSQLRAAIGLET
                     DRDYSTSSDEVNENWKIDTRKHAFESQVGATDDLRYGMSVNPHMKVYLTHGVFDLVTP
                     FFTAERIRRLMKLDEERKRLLTVRHYDGGHMFYTWDESRKAFARDLEVFYRASPPTGA
                     "
     gene            complement(8686..9687)
                     /locus_tag="P5313_08920"
     CDS             complement(8686..9687)
                     /locus_tag="P5313_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY80525.1"
                     /translation="MTNLKKGFALLAVAALVLLAACAPKTEAAKPAAPTEVRHITMGT
                     AGVGGSNYPTGIAMSALWNANIPGVKAVAIATGGSPHNIDLLRTKDADVAVCRSLEAY
                     RATNGIEPYKEKMPWLRSLTGGLFTDVFQVVAVKASGIKSVADFKGKRIVVGPVGSGG
                     EVDARETLAAYGLTYEDMKVSFVEFAQGIDMMKDGLADAGIIGLAMGASGMQELLLDG
                     KVGLIPITDEALANLRKKNEFLIRRNIPANTYPNQPEAVPTVGTPPDIIIVRDDMPED
                     LVYQMTKTLYTNLPAIHAVSALLTQFTKDLVLPEDQMLIPYHPGAKKYFVEMGWLKK"
     gene            complement(9846..11810)
                     /locus_tag="P5313_08925"
     CDS             complement(9846..11810)
                     /locus_tag="P5313_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRY80526.1"
                     /translation="MEKKAETAAAAEAAPDGEKILQQIDRESGYRRNVDLFWKYVIFA
                     ISVVFISYHMWTSRFGMPEVIKHRAIHVGFILLLVFLYFPSSKKSPRNRPTVFDLILA
                     ALSIGMVLYTLLQRDAFLKRGGVAEPMDLIVGGVAILLVLEACRRSVGPQLLILCIVF
                     LLYAWGGRYIPGILSHRGHSLERIIYQMYLTSQGIFGMPIGVSSTYLIIFVVLGAMLD
                     KSGLSKLFNDIALAIGGRLTGGPAKVSVVASALVGMISGSAATNVATTGAFTIPLMKK
                     VGYKPYFAGAIEAAASTGGQFMPPIMGSVAFIMAEFLGVRYLTIAGAAIIPALLYFAG
                     VFFQIDLRARSMGLRGLSKEEIPNVWKTILRYGQMIIPIFVLVYLLFEGRTPLYAAFY
                     TVVITALLSWIRKETRIGAKELRDVAVNSARSSLSIGVAMANAGFVVAVLSMTGIGII
                     LADNIVRLSAGQLPIALFLCMVVSIILGMGLPTSACYVIAGSIAVPILTKMGVPPFQA
                     HFFVLYFACLSTITPPVALAAYVGAGMSGASPNKVGWTAFRLALAGFIVPFFFIYNPE
                     MLLISDSKLAIIWSAISGLAGTALLAVAVEGYLTKRIIWPLRIVFGAAALMLIAPGLR
                     TDLIGLGLTLTGFAGVKFLPAAKERKAAAA"
     gene            complement(12064..12732)
                     /locus_tag="P5313_08930"
     CDS             complement(12064..12732)
                     /locus_tag="P5313_08930"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY80527.1"
                     /translation="MNKKITAMPIREQLYEAVKESILRNAFIPGSLLQIDRLAEEYGV
                     SATPVREALVRLESDGLVTLIPNKGAQVTEITEKDIREIWEMRRLLEPYAARSSAPAI
                     SEVDISELERLILNLREEPSNTELYIKTDIMLHEMLYFHCPNSYLRETLRKVHEVSYR
                     IRYFAESRKTMHDTVIHEVIREHLDILDILRSRDPDRIVVAVSSHLLNGERRALSALP
                     KSKS"
     gene            12916..14532
                     /locus_tag="P5313_08935"
     CDS             12916..14532
                     /locus_tag="P5313_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY80528.1"
                     /translation="MTVAERLVARLRSAGIRRVFGVPSGSWLYYMDAMRAGGVDFVLV
                     ANEATAGFMADVSARLTGIPGACYGTVGPGATNLSTGVGGALLDRSPLLAFTSEPPEK
                     MLGRTVQMAVDHQALYRPLTKWTTRLAPDRVDQVLDQALQIALSDMPGPVHIGLPEDL
                     GPKETTNSPVAAGLPPVRTLGPASEDALRSIETAMSASRRPVLAVGLSAVRAGLGPRL
                     IRIAEKHRMPIILTPMAKGLVSENHPLYVGVLFHALSDRVAATHKEADLVVAVGYDPI
                     EFNYEDWMPSVPLLCLDTLPADIDRILYPEVTDVTGDIGTSLLRLEALAPLDSRWDLK
                     ELAGRRDAMFRAMEPPPGSFGPRAALAGLREALPEDGIMTCDVGAHIHLVGQMWRTPA
                     PGLQLMTNGWSSMGFGIPAALAAKLCRPDRKVACVTGDGGFHMMAGEMATARRIGLPI
                     VFVLLDDRSLDLIRLKQDKKKMPRYGTDLLDRDYPHADSVFGVPVIRAGNASDYRDAL
                     SKAFKSDGPVIVEASIDASEYDDLILRKHK"
     gene            14543..>15084
                     /locus_tag="P5313_08940"
     CDS             14543..>15084
                     /locus_tag="P5313_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY80529.1"
                     /translation="MDTKDTGKSFAERAMADRLSLVPFSGIRKVFDKVKELEAAGKDV
                     IHWQIGRPDFDTPEHIKAAAAAALKRGEVHYAPNLGIPALRKAIGARTTIDTGVPVDG
                     EKQTCVMAGANEAILVSMLAFINPGDEVLVPDPNWHHYKSCASMAGGIPVEVPVRLED
                     GFSMSPDEVEKRITPRTKML"
BASE COUNT         2708 a         4887 c         4835 g         2654 t
ORIGIN      
        1 ccgcgtccag gcccacgatg tcgtcgtgga gctgggtcat ctccttccac cagaaccgct
       61 ggatgcggtt cttgagctcg atgcccgcgg gaccgtagtc ccaggctccg gcctggccgc
      121 cgtagatctc cgaggactgg aagacgaaac cgcggcgctt ggccagggag acgatctttt
      181 ccatcgtgac gctggaatcc atgctgtgct cctgcttcgc cggacccgcc ggcgcccggg
      241 cgactatccc cgagaggtcc tgatttcggg ccgccgacgg attacggcgg tccgccggga
      301 aggcgtttcc ccgtcccgga ccttgtcggg acggggacag ccgcggacgg ctccccccgg
      361 tggcccggcc gctcctgcgc tcggaccggg cttgggcggg agtataccgg aaagcggcgg
      421 gaggggctac gggggcgtat acggatatca ggaggctccg gacggctcag ggcgcgctgc
      481 ctgcggcacc cagggccgga cctaggattc tgtgcagcag ggcttccagt cggaacactt
      541 cccgggtctt ccgggtgccc agtccgtacc gatcgtcccc ggcccggagc gagtccgcca
      601 gcctccggaa atccaggacc cggtagtcca cgacctgccg gcccctccgt ccgacatgca
      661 ggtagacggg caaggtctcg agggaggtca ccctcgtcgc ggactttccc gagaccgacc
      721 ccttcgagat ccggatacgc gccaggcacg cgagccggga gttcggcagg gtccggtgga
      781 tcgacagcag gtcccccagg gcatagagta tgagcccatc ccggcggacg ccctcggggt
      841 ccgtataggt atggcgctcc gcgggctgga cgttgtgggg gtggttcccg acgacggtgt
      901 cgatgccgag ctcgatcagg cggtgcgcgg tttccacctg gctccggacc gggaaggact
      961 cgaactcgag cccccagtga atcagtgcca cgacggcgtc tgcccccgcc gcccttgccg
     1021 cccgtacctg ccgcgctacg ggggagaggt cctcgttcgg cttgttgaac cgcacgtaat
     1081 tggccaggta ttcccggcct ttcggaagct tgccccagtt gagtccgaag gtgaaggaca
     1141 ggaaggcgac cttcacgccg ttccgctccg caatctccac cgcgtcccgt tcctcggtgc
     1201 tccgggcggt tccgacatgg cggtatcccc gggcgtcgag gaaatcgagg gtctcccgca
     1261 gccccgcctc ccccatgtcc aggcagtggt tgttggcggt ggagaaaaag gtcacccccc
     1321 gtcctccgtc gacgatgcgg tcgaacatct ccggagtgcc gttcaggagt ccgggcagca
     1381 atatactttc gggtatgtag cgagccggaa gcgagggcgc gacgggggac tcgaggttcg
     1441 cgcagacaag gtctccggag aagaaaaagg gcctcacgtc atcccacagg gattccgtgg
     1501 tttcgggtcg gattcccggg tagggcatca tgtcccctcc gacgcagagg gtgaatccgg
     1561 cggcctcctc gaacccgtcc cgggggcgaa agtcgagatc cttgcctcgg aaatgctcct
     1621 ccaggcccgc gccgggttcc ggttcctcga tcgggcccca gtaatacttg tatccccacc
     1681 agtatttctc gtccgcgtcc atgaacggcc cgttgccctc caagggacgc gacgcggaga
     1741 acgtcttccg gtgccggaac ccgtagatcc ggcatgcgac cttcaccttg aaatccgcca
     1801 tgtcgtgcct tcctcggtgt acgcgaccgc cgcggaccgg gttcactccc ggcccgcatc
     1861 gaggcgggtc ggtcagggtt tcgcttcctt ggacaccacg gtgatctgtc cccgcttgaa
     1921 caggaagcgg gctcccagga tcgttatggg caggaggacc aggatcgtta cgccctccca
     1981 gcccttgatc gggaatacca ggtagtggat gacggccacg accaggagcg gaaggatgaa
     2041 ggccttccac tgtccccgaa ttaccacggg acccgagtcg ccgccggtca ggaacgcgac
     2101 gaccagggct ccgaacaggg ccggaaggac gttcgccgtg gcgatccgaa ccgaggggtt
     2161 cgcgaacacc ggggcgaggg gcgccagaag aagggcggcg gccatgatga ccgcggtcgt
     2221 cacgatcgag gagatcgcca cggagatggt tgacaggacc tccccttcct cgctggccgg
     2281 ctggactccc gcgagcttga gggcgttcat ggcgcaggga agctttatgt tggagacgtt
     2341 tccggtctcg aaggtgaggt aggatgctcc cgagcccaga aggggcacga aactcacgat
     2401 ctcggacagg gcgataggag tcaggatcag gaacaggccc atcgtagcct tgagagccgt
     2461 ggccaggctg gggaaaatgt cgtagaccgc gcagaccacg gtggggatgg cgaacatcag
     2521 cacgaagaag atcgcgaagc tgatccttcc gaaccggttg attcgggtaa aatactgctc
     2581 tcgggtttcc atgggttccc tcctacttag ccagcgccgt ccagagcacg gcggagcaca
     2641 taccgaggat gaggctgaag gacaggatga agttccccag ccagacgatc ttgaacttcc
     2701 gggcaaggaa gccctggaag agggtcagga cgatactcgt gaagaaggta gccacagcca
     2761 cgacgccctg tccgaagaag ggcgccacga acaccgcgag gacagcgtag tagagcacgc
     2821 cgatggctac gatcccgaaa ctgcccgcct tggccttcat cttgaccgcc agggaatcca
     2881 ccttcttgat gaacagaatg tccaagatga tggcgcccgc tagccctccg gtcatggcgt
     2941 acatgatggc gcccaggggt tccggtccgg ccttggaggc gtccgcgagc ttggcgaatc
     3001 ccaaggcgct ggaggccatg ttggcggcca tgagctcgta gctgacgctt ccgatgaccg
     3061 agagccggaa ccagggccag gcgatcccca ggctcgcagc catggcggcc aggcccaggg
     3121 ccacggcgat ggagggggcc aaggtgaaga ccgcggagga cttgatggtg ttcgagacca
     3181 ccttcggatc aatgccgagt tcccgggctc gtttcaggga catcctcagg aacaggacgc
     3241 actggaccag gacataggcg atgcccgccc cgaccagcac gaaaagcaga ctcccgtttg
     3301 ccacatcgaa gaaattcatg ttcccctcct tgattcaaac gatctctgcg gcttctctcg
     3361 gttcagaacg gtacattgct cacggaccga agcacgtcct ccggttcgat ggtttccggg
     3421 atggggctgg aataggaatc gccgaccttg gacgcatgct cctcccgggc ccgctccacg
     3481 agtgcgggat ccaggaacgc cgcggcggcg gccaatccca gggcacggcc cgcggcgagc
     3541 atccccttgc gcccgatcga ggaacccgcc tgggcggtgt tctgccagga gtgcgcctgg
     3601 gtacccaggg ccgccgcggc gtagacgatc tgtcccgtgg gcacgacctg gctcacgtct
     3661 cccacgtccg tggagcccgg gaggcagata tccccccgga acagggggcc caccaccgaa
     3721 tccagtggag agccgccgat cgcctccagg acggcggggc cttgggtcat ccggagctgt
     3781 cccaggatcg cggccttctc cgcctccgtg agggtggccc ggaatttctc cgccaattcg
     3841 agatcggccg cgtcgaaggc cgggccgccc gcttcctcca tgcacttctg aaggtgttct
     3901 ccgaggacga gattcggtac gtagtcggac aacccctgtg cgaaaacgat ctccaggttc
     3961 gtccccgtca tcagggccgc cccccgggcc acgtcgcaga gcctctcgta gatttcccgg
     4021 gcctgggcga tccggggggc ccggcagtag tagaaggact ccgcggaagc ctggacaacg
     4081 ttgggcgccc tgcccccggg gttggtgatg gcgtagtgga gccgggcttc ggggacgatg
     4141 tgctcccgca ggtagtttgc gcccacgttc gtgagctcca cggcgtccag ggcgctccgc
     4201 cccaggtggg gggaagccgc cgcgtgggaa gccttccccc ggaaccggaa atagaccgac
     4261 aggttggcca gggtccgggc ctgccacacg gcgttgatgt cgctcgggtg ccagcagaag
     4321 gccaggtcca cgtccccgaa gacccccgcg cggaccagga aggtcttccc gcagccgaac
     4381 tcctcgccgg gacatccgta ataccgaacc gtgccgggta ctccggaatc ccgaaggaag
     4441 tccttggccg ccaccgcggc cgccagggct ccgatcccca ggaggttgtg gccgcagccg
     4501 tgccctgcct tcccggaatc gagttccttc tgctccggaa gggatgccgc ctgggaaagt
     4561 cccggcaggg cgtcgtattc ccccaatacc gcgacgaccg gactcccgga accgtagctt
     4621 ccgacgaagg cggtttccat ccccgcgatt ccccggtcca ccgtgaaacc ctccgactcc
     4681 agacgatcgg cgatcgcggc ggcggaggcc cgctcctgga atcccgtctc cgcgaatccc
     4741 cagatccggt cgctcaaggc ctcgtagagc gcccggtttc gatccacgct ttcaagtacg
     4801 acgcgcttcc cttccattcc ctgcctccga ttctctccag cccggtccga gattgacagc
     4861 ggacgtcggt cgggggatac ttttcgggtc ggtgcttgtt tttcaacatg tgtccgaatt
     4921 acaggtcgac tataaacccg ccttgccggg ctgtcaacga atttcaaaca gctgtcgaat
     4981 ttcaagcgac gctgatcgac gggggaccac catggcgtac agaaaaagcg gggaaactcg
     5041 ggagcgtatc ctaaaggccg ctgcccggct gttctcggag cggggctact acgagacggg
     5101 catcggagac atcgcccggg aggcgggcat cggcagggct tccttctact accacttcac
     5161 ggacaaggag aaggccgccc gggcggtctt cgacacctac gtggagggta tctacgcggc
     5221 ggcggaacgg gtcgtgccga aatccgacgc taacgcctcc gccgaggaac tccgccgccc
     5281 cctgctcctc agcgtgttcg ttgagtacat cctgctgttc cggttcatag ccctgaacaa
     5341 agccacccac gcggtctact acgacctggt gaacttcgcg gactacgacc gggagaacat
     5401 cgatcggttg aagcggacga ccttcaagga cacggtcttc ctggcctccc tctacggaag
     5461 gcgtctcacc gaggccgaac tcgtcgcctt catcgtcacc accaacgccg cggcaaaatc
     5521 catcttcaag gctgtgtcca cgggaatcct cgacttcgac ctgtacgagg cttcggatta
     5581 cttcttccgg catgccgtcc ttccggacat tcccatcccg gagccggagt accgaaacct
     5641 gctgatacaa gcgttctcac tatgcgagtc cgtcgacctg tcttgatccg gatcgtcccg
     5701 gccttgaccc gcctcttccc ctccgctacc cttcgcacac catggcagtt tctgtcgaaa
     5761 tgagggacgc gaccgtcttg ctgggcggcc gtccggtgct ccgggaggcc tccttcaccc
     5821 tggaggagga cgaacacgtc tgtatcctgg ggccgaacgg ctccggaaag tccaccatcg
     5881 tacggctcat ctccggggac ctctttcccc tgtaccggga acccgcggcg gttcgcctct
     5941 tcggacggga gcgctgggac ctcttccaaa ttcgcggccg cctggggatc gtctcggaca
     6001 ccctccaggc atggcaggcc gcgggcgggg aaagcgtgcg ggacaccatc ctttcgggct
     6061 tcttcggagg cgtgggcctc ccgatgcggg gcgctccgga gacctggagg gagaggaagg
     6121 ccgaggaggc ggcccggatc ctggggatcg aggagctcct gggccggctg gtggggaccc
     6181 tctcctccgg gcagatgcgc cgggtcctgg tcgcccgggc cctggttcac gagccgaaaa
     6241 ccctgctcct ggacgaaccg tacacctccc tggacatcgc cgcccgccac accttcgcag
     6301 agcgggtccg tcacctggcc gcccggggcc acgccatcat cctcgtgacc cacgagctgt
     6361 cggagatccc tcccgagatg gaccgggtca tcctggtcaa ggagggccgc atcctggcgg
     6421 acggtccgaa gcgggaggtc ctggtatccg gagtgatatc cgagctcttc ggcatccccc
     6481 tgaccgtccg ggaggaaaac ggccggtacc gggcggtccc ggcctgaggc ccgcccgggc
     6541 cggaatccac gcacgcggga ggtaacgaca tgatcctgga agcccgtacg gcccggctcg
     6601 tcaagagcca ggacctgaac caccatcaaa ccctgttcgc gggccgggcg gcggaatggt
     6661 tcacggagac ctgcttcatc gccgcggccc ggttcaccgg ggatcccgcc ggcctcgtct
     6721 gcgtgcggat ccacggcctc accttcacga agcccgccca tcccggcgac tgcgtggaga
     6781 tcgtggcccg gatcgcccac acgggaagga ccagcgtcac ggtctccggc gagatcttca
     6841 tcaacgaaac ccccacgccc acggtcaagg gcttcgccac cttcgtgcac gtggacgccg
     6901 acggaaaggc cctgccccac gggctgtccc tgcccgcgga ctacctcgcc cggcaccggt
     6961 cgctccacga ggaggcgctg tcccttcccc gccagggata gcgccggggt aacccgccgg
     7021 ccggatcagg cacccgtcgg cggactcgcc cggtagaaca cctccaggtc ccgggcgaag
     7081 gccttccggc tctcgtccca ggtgtagaac atgtgtcccc cgtcgtagtg ccgcaccgtg
     7141 agcaggcgct tacgctcctc gtccagcttc atgagccgtc ggatccgctc cgcggtgaag
     7201 aacggcgtca cgaggtcgaa cactccgtgg gtcaggtaga ccttcatgtg ggggttcacg
     7261 ctcatgccgt agcggaggtc gtccgtcgcg ccgacctggc tttcgaaggc atgcttgcgg
     7321 gtgtcgatct tccagttctc gttcacctcg tcgctggagg tggagtagtc ccggtcggtt
     7381 tcgagtccga tcgcggcccg aagctggctg ttcacgccgg ccgtgaagat gcgttccacc
     7441 acgtggaggg tcggatccgg accggaccaa tcgtcccggt ccgggtaggg atcccggacc
     7501 gtggcgctgg cgtcgtagag ccccaggacg aggccccggt cccggagcag gctcttggcg
     7561 aagtattcga tcgaaacccg gccgcccttg gagcggacga tgtcccgggg aagtccgatg
     7621 aaatccgcgg ccgcgtccag aacccgtacc cgccgggctt ccccgaccag gtcccccgcg
     7681 gccagcaccg gaaggagttc cgtgaccgca aattcggaag cccttcgggc gtagtcctgc
     7741 cgggactccc cgggcttcca tttccgggcc cggtcgtgga agcaggccgc gcccgccatg
     7801 gtcgggaagg tatccaccca gggcaggtcg tcgtagtccg aggggtccag cagggcgaac
     7861 tccagggccg gtgagatcag gaccgcgccc gccagggcca ccccgtagtc ctgctgcaaa
     7921 aggcgcacga gcttggccac ccggaagccg ccatagcttt ctccggccag gtacacggga
     7981 ctgtcccacc gcttcttgcg ggacaggaat ttccggatga actcgcagat ggactccagg
     8041 tcccgcttga ggccccagta ctccacgtcc ttcttttcat ccgacccctt cttttccggg
     8101 tccttcttgg cctccgcgtc cgggatcatc cgggacagtc ccgtgccgat cgggtcgatg
     8161 aaaaccaggt ccgtgaaccg gagccaggtc tcctggttgt cctccagctt gtgcgggggc
     8221 ggttcgggct cgccgcgggg accgaaaacc gccctccggg gacccagggc gccgaaatgt
     8281 aggtacacgg aggaggatcc gggcccgccg ttgaacacga aggtcacggg gcggtcacgg
     8341 ccgtccggca ggaaatagcc cgtatagaac atctccgccg cgggcttttc atccttgcgc
     8401 aggatgatcc agtcggcctc ggcctggtac ggaagttcgc ggccgtcaat tcgtatcgag
     8461 agggatgccc gcgccccctt gggcggattg tacgccgttt tggattccgt caggccgggt
     8521 tccttgccta cggtttcagg attgtgttcc atgcgcgctc cttttctgga acagggtact
     8581 cctccccggc ccggcgcgca atacggtgcc aaggaaataa aaatgccgcc cccgggaggc
     8641 atcacggggg cggcggaaac gggagaatct acccgagacg agaggctact tcttcagcca
     8701 gcccatctcc acgaagtact tcttggcgcc ggggtgatag gggatcagca tctgatcctc
     8761 gggcagcacc aggtccttgg tgaactgggt caacagggcc gagacggcgt ggatggcggg
     8821 caggttcgtg tacagggtct tggtcatctg gtacacgagg tcctcgggca tgtcgtcccg
     8881 gacgatgatg atgtccgggg gggttccgac cgtcgggacc gcctcgggct ggttggggta
     8941 ggtgttcgcg gggatgttgc gccggatgag gaactcgttc ttcttccgga ggttcgccag
     9001 ggcttcgtcc gtgatgggga tgagtcctac cttgccgtcc agtagcagtt cctgcatgcc
     9061 cgaggctccc atggccaggc cgatgatgcc ggcgtccgcg agaccgtcct tcatcatgtc
     9121 gatgccctgg gcgaactcca cgaagctcac cttcatgtcc tcgtacgtca ggccgtaggc
     9181 cgccagggtt tcccgcgcgt ccacttcgcc gccggaaccg acggggccca cgacgatccg
     9241 cttgcccttg aagtccgcca cgctcttgat gccgcttgcc ttaacggcca ctacctggaa
     9301 gacgtccgtg aacagcccgc ccgtcaggga ccggagccag ggcatcttct ccttgtaggg
     9361 ttcgatgccg ttcgtggccc ggtaggcctc cagggagcgg cagacggcga cgtccgcgtc
     9421 cttggtgcgc aagaggtcga tgttgtgggg gctcccgccg gttgcgatgg ccacggcctt
     9481 gacgcccggg atgttggcgt tccacagggc cgacatggcg atgcccgtgg gatagttcga
     9541 accgcccacg cccgccgtgc ccatggtgat gtggcggact tccgtgggag cggccggctt
     9601 ggcggcttcc gtcttggggg cgcaggccgc gagcaggacc agggccgcca ccgcgagcag
     9661 cgcgaatccc ttcttcagat tcgtcatctt ccttcctcct aatctaccgt ccgcgtgcga
     9721 cggatctcga actatatcca gccatccacg atggccttgg cacttttcga gcccggtacg
     9781 ccggacgggc cggcgcgccg gggacggacg cggaacccca gggtcggggc cccgctcctt
     9841 cggggtcatg ccgcggcggc cttacgctcc ttggcggcgg gaaggaactt cacgcccgcg
     9901 aagcccgtca gggtcaggcc caggccgatc aagtcggtcc ggaggcccgg ggcgatgagc
     9961 atcagggccg cggcgccgaa cacgatacgg aggggccaga tgatccgctt cgtgaggtat
    10021 ccttccacag ccacggccag gagggcggtg ccggcgagcc cgctgatcgc cgaccagatg
    10081 atcgcgagct tggaatcgga gatcaggagc atctccgggt tgtagatgaa gaagaacggc
    10141 acgatgaacc cggccagagc caggcggaag gcggtccacc ccaccttgtt gggggaggct
    10201 ccggacatcc ccgccccgac gtaggcggcc agggccaccg gcggggtgat ggtagacagg
    10261 caggcgaaat agagcacgaa gaagtgggcc tggaaggggg ggacccccat cttggttaga
    10321 atgggcaccg cgatggaccc ggcgatcacg tagcaggcgc tggtcggcag gcccatgccc
    10381 agtatgatgg acaccaccat gcataggaac agggcgatcg ggagctggcc cgctgatagc
    10441 cggacgatat tgtccgccag gatgatgccg atgcccgtca tggagaggac ggcgaccacg
    10501 aacccggcgt tggccatggc cacgccgatg ctcaaggagc tccgggcgga gttgaccgcc
    10561 acatcccgca actccttggc gccgatccgg gtctccttgc ggatccagga caggagggcg
    10621 gtgatcacca cggtatagaa ggccgcgtag agcggggttc gcccttcgaa cagcaggtac
    10681 accaacacga agatgggaat gatcatctgg ccgtagcgca gaatcgtctt ccagacgttc
    10741 gggatctcct ccttcgagag tccccggagt cccatggacc gggcccgcag gtcgatctgg
    10801 aagaagaccc ccgcgaagta caggagggcc gggatgatcg cggctccggc gatggtcagg
    10861 tagcgcaccc ccaggaactc ggccatgatg aaggccacgg agcccatgat ggggggcatg
    10921 aactgcccgc ccgtggaggc ggcggcctcg atggcccccg cgaagtacgg cttgtagccg
    10981 accttcttca tgagggggat cgtgaaggcc cccgtggtcg ccacgttggt cgccgcgctc
    11041 ccgctgatca tgccgaccag ggcgctggcc accacggata ccttggcggg accgcccgta
    11101 aggcgtcccc cgatggccag ggcgatgtcg ttgaacagct tggacaggcc gctcttgtcc
    11161 agcatggccc cgagtaccac gaatatgatc aggtaggtcg acgaaacccc gatgggcatg
    11221 ccgaatattc cctggctggt caggtacatc tggtagatga tccgctcgag ggagtggccc
    11281 cggtgggaaa ggatccccgg tatgtaccgt ccaccccagg cgtacaggag gaagacgatg
    11341 cacaggatga ggagctgggg ccccacggac cttcggcagg cctccaatac cagcaggatc
    11401 gccacgcctc ccacgatgag atccatgggc tccgccacgc cgccacgctt gaggaaggca
    11461 tccctctgca gaagggtgta caggaccatg ccgatggaca gcgcggccag gatgagatcg
    11521 aacaccgtgg gccggttccg gggcgatttc ttcgaagaag ggaaatacag gaagacaagc
    11581 aacagaatga atccgacgtg gatcgctcga tgcttgatca cctcgggcat cccgaaccgg
    11641 gatgtccaca tatggtagga tatgaagacc acggaaatcg cgaagatcac atatttccag
    11701 aacaggtcga cattcctgcg atatcccgat tcccggtcga tctgctggag gatcttctct
    11761 ccatccgggg ccgcttcggc tgccgccgca gtctctgctt tcttttccac gcctttcccc
    11821 ttgccgggag cgggctcccg gtatcgttgg cccgaatcac ttcgggctgc atatggatat
    11881 ggatactagt tgatcgatat cctatatcct atatcctata tccagtgtat catgagtata
    11941 gtatccgtca agtaaaaaat aaagggctac gcgaagaagc agggacaatc cggcttaggg
    12001 ctaaatatgg aagggatttt cggacaattt cttcgccgaa cggattggat ggagggatct
    12061 gtcctaggac ttgctcttgg gaagggcaga aagggctctt ctctcgccgt tcaacaggtg
    12121 ggagctgacg gcaacaacga tccggtcggg gtctcgggaa cgcaggatat ccagaatgtc
    12181 caaatgttca cgaatcacct cgtgaatgac cgtgtcatgc atggtctttc tgctctccgc
    12241 gaagtatcgg atccggtatg agacctcgtg cacctttctc agggtttccc gaagatagga
    12301 attcgggcaa tggaagtaca acatctcgtg tagcataata tccgtcttga tatagagttc
    12361 cgtattggaa ggttcttccc ggaggttcag gatcaggcgc tcgagttcgg agatatccac
    12421 ttccgaaatg gccggagcgc tggatcgggc cgcatagggt tccagaagcc ggcgcatctc
    12481 ccatatttcc cgaatatcct tttccgtgat ctccgtgacc tgggcgccct tgttcgggat
    12541 gagggttacc aaaccgtccg attccaggcg taccagggcc tcccggacgg gggtggcgct
    12601 gaccccgtac tcctccgcga ggcggtcgat ttgcagaagg gaaccgggga tgaaggcgtt
    12661 ccgaaggatg gactccttga cggcttcgta gagctgttcg cggatcggca tggcggtaat
    12721 tttcttgttc atgatgtcac cgcgagcatt atatatcata tacttcatac tgtccatgaa
    12781 aatactctga acggaccaca ctggataggg cggattctac ccaatttcaa ctttcacctt
    12841 gccaatactg tacaagaatg ttataaatat atgatatatg atcgtggata ttcccctttt
    12901 tcaaggagtg catcgatgac tgtcgccgag agattggtag cccgcctgcg cagcgcaggt
    12961 atccgacgag tattcggcgt acccagcggc tcgtggctct attatatgga tgccatgcga
    13021 gccggcggcg tggatttcgt gctggtcgcg aacgaggcta ccgccgggtt catggccgat
    13081 gtctccgccc gcctgacggg tatcccggga gcctgctacg gtaccgtggg acccggtgcc
    13141 acgaacctca gcacaggggt cgggggagca ctcctggacc gctcccctct cctggcgttc
    13201 accagcgagc cgcctgagaa gatgctgggc cgcacggtcc agatggccgt cgaccaccag
    13261 gccctgtacc ggcccctgac caagtggacc acccgcctcg ctccggacag ggtcgaccag
    13321 gtcctcgacc aggccctgca gatagccctc tccgacatgc ccgggcccgt acacatcggc
    13381 ctgcccgagg atctgggtcc caaggagacc acgaactctc ccgtcgccgc gggtctgccc
    13441 ccggtgcgta ccctgggccc ggcctcggag gatgccctcc gctcgatcga aaccgccatg
    13501 tccgcttccc gcaggcccgt cctggccgtc ggattgtccg cggtccgtgc gggcctgggt
    13561 ccccggctga tccggatcgc cgaaaagcac cggatgccca tcatcctgac ccccatggcc
    13621 aagggcctgg tatcggaaaa ccatcccctc tacgtcgggg ttcttttcca tgccctgtcc
    13681 gaccgtgtgg cggcaaccca caaggaggcg gacctggtgg tggcggtcgg gtacgatccc
    13741 atcgagttca actacgagga ctggatgccc tccgttcccc tgctctgcct ggacaccctt
    13801 cccgcggaca tcgaccgaat cctctatccc gaggtcacgg acgtgacggg agatatcggc
    13861 acatcccttc tcaggctgga agccctggct cccctggatt cccgttggga cctcaaggag
    13921 ttggccgggc ggcgcgacgc catgttccgg gccatggagc cgcctccggg atccttcggc
    13981 ccccgggcgg ccctggccgg cctgcgggaa gccctgcccg aagacggaat catgacctgc
    14041 gacgtcggag cccacatcca cctcgtcggc cagatgtgga ggacccccgc cccggggctt
    14101 cagctgatga ccaacggatg gtcgtccatg ggcttcggca tccccgccgc cctggccgcc
    14161 aagctctgcc gcccggaccg gaaggtggcc tgcgtgaccg gagacggcgg tttccacatg
    14221 atggccggag agatggccac ggcccgcagg atcggactac cgatcgtgtt cgtcctgctg
    14281 gacgaccgca gcctggacct gatccgcctg aagcaggaca agaagaagat gccccggtac
    14341 ggtacggacc tcttggaccg ggactacccg cacgcggatt ccgtgttcgg agtccccgtg
    14401 atccgcgccg ggaacgcctc ggattaccgg gacgccctat ctaaggcgtt caagtcggac
    14461 ggtcccgtca tcgtggaggc ctccatcgac gcctcggagt atgacgacct gatcctgcgc
    14521 aagcacaagt aggaggaacc acatggatac caaggatacg ggaaagagct tcgcggagcg
    14581 ggcgatggcg gacaggctct cgctggttcc gttttcgggg atacgcaagg tcttcgacaa
    14641 ggtgaaggaa ctggaggcgg ccggcaagga cgtcatccac tggcagatag ggcggccgga
    14701 tttcgacacc cccgagcata tcaaggccgc ggcagcggcg gcgctgaaac ggggcgaggt
    14761 ccactacgcc ccgaacctgg gcatccccgc cctgcggaag gccatcgggg cgcggaccac
    14821 gatcgacacc ggggtgcccg tggacgggga aaaacagacc tgcgtcatgg cgggcgccaa
    14881 cgaggccatc ctggtttcca tgctggcctt catcaatccc ggcgacgagg tcctggtccc
    14941 ggatcccaac tggcaccact acaagtcctg cgcctccatg gccggcggga tccccgtgga
    15001 ggtgcccgtg cgcctggagg acgggttctc catgtccccc gacgaggtcg agaagaggat
    15061 caccccgagg accaagatgc tctg
//
LOCUS       DAOTEI010000078        15039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2724_length_15039_cov_5.048185, whole genome shotgun sequence.
ACCESSION   DAOTEI010000078 DAOTEI010000000
VERSION     DAOTEI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 15039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15039
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_15039_cov_5.048185"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="P5313_08945"
     CDS             complement(<1..526)
                     /locus_tag="P5313_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRY80530.1"
                     /translation="MRDLVIRAPDDFHVHFRSGPGLEKFVRRTAALFDRALPMPNTLP
                     PLADAATLRAYAASVRAAAPDLDLVLSFKLLPGMDTETVRSLAGAGARVGKYYPAGAT
                     THSSDGIREPGEIREALAAMEDAGLVLSIHGEDPAAPVLERERAFLPVVRGLLDDYPR
                     LRIVLEHLSTREAAE"
     gene            complement(519..2141)
                     /locus_tag="P5313_08950"
     CDS             complement(519..2141)
                     /locus_tag="P5313_08950"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY80531.1"
                     /translation="MTTPESRTRIRHEIRTLLNHIVGYADILLEDAAGRRESELETAF
                     GDLREKALSLREPLLQFFAAPGEGAPAAESREPLKRRIYGLLYDAIAQIQAAKRACQS
                     EDLSLYIPDLQKILEASNRTAELFESALAHAFDAEELSEESLAGIEESAGPSRLAGSI
                     LVVDDDAFNREILTRHLERQGHSVHQASDGAQALNALDQGSYDLVLLDIMMPGMNGYQ
                     FLERIKAAPNQRDIHVIVISALEESSSVARCLRLGAEDYLPREFDPVVLRARIESCLE
                     RNRLRARERLSLRAVAAAQNHLAAELRRAAEYVRGLLPRKVRWRELRTDWVFLPSRDL
                     GGDAFGYTRLEDGRIALYLLDVSGHGIEAALLSVSLLHRVNSLSRAEPELWDPRRILS
                     DLNASYRGEEQNNLYFSAWFGLWDPRTRRLRYASAGSPPAALLAPDEPVRELRTEGPA
                     VGVDDAAEFPGAETEIPPNSVLYLFSDGIYEVRGADDRILGLPGFLSILEESNRSLGT
                     AGVEGIVRAVRAASGRGQFEDDVSLVGFRFDA"
     gene            complement(2147..2281)
                     /locus_tag="P5313_08955"
     CDS             complement(2147..2281)
                     /locus_tag="P5313_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80532.1"
                     /translation="MAFKLDGAKFPTLEELVEALYPLYADRMTEEEFRKYAEEHAERT
                     "
     gene            2432..3715
                     /gene="murA"
                     /locus_tag="P5313_08960"
     CDS             2432..3715
                     /gene="murA"
                     /locus_tag="P5313_08960"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRY80533.1"
                     /translation="MYKYRIEGGFPIKGRIRASGNKNAALPCIAAALLAEEPVILRNI
                     PEIEDAQVMLETASRLGARVEKLDPGVWRIDASGPLGTEIPEEQSRKIRASILFAGPL
                     VARTGRCVLPPPGGDVIGRRRLDTHLLALEELGARIEVDGKFVFTANKLVGADVFLDE
                     ASVTATENAVMAAVRAEGRTVLRNTASEPHVQDLCRMLVGMGASISGIGSNILEIQGV
                     PALGGTDFSIGPDFMEIGSFIGLAAVTHGELDIEGVRSDELRMIKLGFAKLGIRWTVE
                     GDLLRVPAGQVLKVVPDLGGQIPKIDDAPWPGFPPDLTSIMTVVATQVEGTSLIHEKM
                     FESRMFFVDKLIGMGARIILCDPHRAVVSGPSRLSGSELVSPDVRAGMAMVIAALCAE
                     GTSMIKNVYQIERGYERITERLRSLGARIDRIEDC"
     gene            complement(3712..4470)
                     /locus_tag="P5313_08965"
     CDS             complement(3712..4470)
                     /locus_tag="P5313_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRY80534.1"
                     /translation="MRPTEAARAGTPALLGALAGGILAGFYGVPAGALLGVMGAEILR
                     TQRLRAELASYLENPDSSPPPRSEPAPGAALLAGLAAAEARRLGIPPESAGQVLRDSA
                     SAPRGLAAWTARAASAVRPSGDLDRSIAVLTAAFDGRADRALRPLAADAFFALRRMNS
                     RDLGPGEDLDTSSRLAALGVPEEEARLARARHFPDYRDDWDVLGLPPGSPTDEVRRAW
                     KRLSRLHHPDGPAGNEEKFREAREAYERLSRARA"
     gene            complement(4457..6199)
                     /locus_tag="P5313_08970"
     CDS             complement(4457..6199)
                     /locus_tag="P5313_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80535.1"
                     /translation="MAVRDSESRSAVAVAVLLGFAAAFVVALLEFRSSDIIARFAIRW
                     ELARAASLLAAWMPPLLFAACAASMSLHREQGLRFAELLRPVLVPALILALLFSALEL
                     TVLPGVRRAQTVYESLSTLFRDALADAETALGDGDLARAQERASLAGAIDSTETAYKL
                     LNEKVQRAVLRARQSEDTSPRKTEAPPAPPDDGASAYEFYQRALAFYRRGDFYSAHWY
                     AGKSLILDPSRRDARKLQADAWERITTPPENPADKARAAFHERKAEAYALLQSEAYLE
                     AYRAFTDLNREDPGDADVVRYRKESLDRLAQTAFFGEDFRRAFAGQSREGLAVRVVSE
                     GVDHVLYASRVAVAGPFLYLEEFEYMTVGENGPVLHVQAPYARLRGLPAAGSEPPGKE
                     APPPASAVSLRMVERGSPRQASRPRFLKGSMVPPDSTVLVLPMDFEALSILLDLQDQA
                     ERIPFFRLAAGARIAPAYGIDPSPYRMETALRISIPVMLMTLVLLGTALGARFRRDEE
                     PGRLRMMLTLPLLTALASIPMAAAGRAGQYLLGRLFADLPSTGALAVWAGFLVSLLAL
                     SFLAVGRLGVHAPD"
     gene            complement(6183..7109)
                     /locus_tag="P5313_08975"
     CDS             complement(6183..7109)
                     /locus_tag="P5313_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80536.1"
                     /translation="MKRGLSVAGRYLAWFLCAVIVETLILAAGPLMKSASGGSSFQLS
                     FWAGYAVESLPVSVSIALLGAGFLATRTLRHRLSGYLILGTLTAAVLSGGVWVSRALT
                     TPPEPFPAGPTASVPASSPARHFVRESSGDTATGIIYVRPSGEGPRLAWAASGTYRRD
                     RGILMADRTPLPLGNPGSSSDPLPGFRELRTWYLELGRGNLFEALAAALGVAALICGL
                     WPLSRLFRWPLVGTFAALAGLVLLGTLHGIFRSPAAGDLASLVGLRVPATLRAGILAG
                     ACGIALVFLDFALCRPFSAARGGARRNRGGPR"
     gene            complement(7453..8064)
                     /locus_tag="P5313_08980"
     CDS             complement(7453..8064)
                     /locus_tag="P5313_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80537.1"
                     /translation="MRVLVIDPGLKHVEEERNAEGPLSFFFSPDLGSPLVCDLIVLPA
                     GLLLEGAGADRDVLRIAYGPASLTADAFAEGCADYLREPWDLEELEARALRFLTWRFR
                     LGEHPFTYSTGRLAGAAGTVALPEGERRALELLLHSLGRPVPRKAFLRVRHGPGPSPS
                     RRPDMTICRLRSRLARAAGDSRAGSFLAAVRNYGYRLDGTPCA"
     gene            complement(8072..8770)
                     /locus_tag="P5313_08985"
     CDS             complement(8072..8770)
                     /locus_tag="P5313_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HRY80538.1"
                     /translation="MGIFSRFKTVVSSNINDMIAKAENPEKMLNQLLLDMNQQMVESK
                     KSVAMAIADEKKIERELAEQKRQADEWEKKAMLAVRAGRDDLAKEALVRKQEYEGYFL
                     QLKPQWEAQKASVEKLKESLRQLQNKIDEASRKKNILIARAKRAEAQEKIQKTLTTMS
                     GNTSAFDTFDRMAKKVDELEAKAEAAKELEDLSKDTSLEKQFAQLESSGASADLLLEE
                     LKAKMLTEDAGTKN"
     gene            complement(8794..9177)
                     /locus_tag="P5313_08990"
     CDS             complement(8794..9177)
                     /locus_tag="P5313_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRY80539.1"
                     /translation="MAGLSRVESYLISLEIQYEEISPNAWLVNDEARGLTQVVITQAD
                     PVVALRTKVMAAPKEHRQELFETLLRLNASDLLHGAYALEGDDIVLVDNLELETLDRS
                     ELEAALDAVGFALAQHYAVLGKFRA"
     gene            complement(9228..9779)
                     /locus_tag="P5313_08995"
     CDS             complement(9228..9779)
                     /locus_tag="P5313_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668265.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HRY80540.1"
                     /translation="MNNNDIVPGFDDEKDDSLKIRLQKVDPVENCLTLFLTGYIDTYN
                     SNFFQKRVSKAIDSGFVRLIFNCGGLNYVSSTGIGSFTAFLKSVKPRGGDIVLLEIQP
                     KVYEVFQLLGFSQFFNIKDNLEEAMGFFRQGDQGSSQSVFPKIFSCPICNKKLKAAKP
                     GRFRCSECKTILAIDNAGQVFLG"
     gene            complement(9990..10736)
                     /locus_tag="P5313_09000"
     CDS             complement(9990..10736)
                     /locus_tag="P5313_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY80541.1"
                     /translation="MQRNADPPLAGRRAFILGGTGGIGRHIARALGLRGARLDVHGRE
                     GRKLESALAEFREAGIEAEGSAFSLDSLAGTGPLLDRAGAADILVAAWGPFLQKPLHE
                     TAPEEWEAAVAFNLALPGALVSAALPGMRSRRWGRILFLGGTRTDGIRGFRTNAAYAA
                     AKTGLGVLAKSIALEYARDGIAALVLCPGFVRTEYLTPETLEGLAAKTPGGSLTDPGV
                     FGDLAADLLCRDPPLWNGGILTADEGLAAW"
     gene            10876..11661
                     /locus_tag="P5313_09005"
     CDS             10876..11661
                     /locus_tag="P5313_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRY80542.1"
                     /translation="MKSPPEFVVVLCRAREPGNVGAACRAMAAMGLGSLVLADCPDHP
                     PEPVKTFALAAYPLFERARRFGTLEDALADCALAAGFTRRTGRLRVREPRDVTEFARD
                     AIRRSGRIALVFGNEADGLSASELALCDLAVRIPTSDVFPSLNLAQAVQIAAWELARA
                     RAEGGRVPPSAEVPGDADGPAAGPDGTSDEAPAARAEILDAVEAAAEDLARAGFFKIS
                     GRPDFVRVLRSLLARAGASGRELEYLRRILGKAAALSGRRPKP"
     gene            11728..12201
                     /locus_tag="P5313_09010"
     CDS             11728..12201
                     /locus_tag="P5313_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80543.1"
                     /translation="MRRPKAPWIAAWAMTFAVLSALPSPAQSGPGGAFDFAASVESLA
                     QAAETGAGLPVGRYVVLTGTVRSTVSQEEGSFSARVELAAGQWIGTSRIVLRTVLVEL
                     AGDSYRDLFDRGSPSFLRPGSAVLVVARVTGTREAPEGGRVAVLEGIHLRRLDTL"
     gene            complement(12221..13402)
                     /locus_tag="P5313_09015"
     CDS             complement(12221..13402)
                     /locus_tag="P5313_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY80544.1"
                     /translation="MRRILHWEEDEEERDILAALLADEYLVRGVGTPAEAEAAAAEEG
                     WDLVILGAGDPVAGDAAGRIRLLKARLPSVPLVCRLPAGDPARTTALLREGAAACVSR
                     DCPGDELKAWLRRVRSSRSTEPSCREPSRPYGEPDPDTRILLGESDCIRGVRRRIRLF
                     ARYDFPVLITGESGTGKDLAARSIHGLSRRASRPFLAVNCGAIPESILESELFGTERG
                     AYTGAVSRPGTFETADGGTLFLDEITELSPGAQAKLLRILENGEIRRLGARSSSSCDV
                     RLISATNVELDRFQGMRFDLLERLETLVLKLPPLRERLGDIPLLARHFLDQAGLAAEI
                     GPDAMELLSGYDWPGNVRQLRNAICRAAVAASESEGGIIRAEHVDFGCRPSALPRVRA
                     E"
     gene            complement(13529..14275)
                     /locus_tag="P5313_09020"
     CDS             complement(13529..14275)
                     /locus_tag="P5313_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRY80545.1"
                     /translation="MDSDRVTRISLLKVPLDILKPEDFDDVIRSMFSDGRNHQIVLLS
                     LWDLLRARRSGEFRTMVNGASLVLPISRSLVRGARFLRREEPVRYMPFDFAIRLLNAL
                     ERWNRSVYLLGSTRRNLSLAEENIHATYPGIRIVGRFPGHYPKQMEQSILQAIRKSTP
                     TLLLVGRGVIGRERWIPRSLKHFNSGLYLWCSDLFDVFAEKVPKPSKALFDRGLEWLP
                     YTLRRPWKVFRILPYLRYRLLLLWYRIRRL"
     gene            complement(14257..>15039)
                     /locus_tag="P5313_09025"
     CDS             complement(14257..>15039)
                     /locus_tag="P5313_09025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRY80546.1"
                     /translation="MRQFVLPPDWSGEPGCVLAGEAARYLLSVLRLSPGDRFPGLDRE
                     GRRYELEIRAVEAGRVELAVRPDTRTGEGRPGGTRLPRLVLVQALPKGRKMDLVVRQA
                     AEAGVARIIPVESSRSVARISDEGGAAAKRARWERIVREALQQSGSSLRTEVDSPRPF
                     QELPGILGKAAPGRIGLYFHEAPLEGAALHRYLSGEPEEIVACVGPEGGFSMDEVRFL
                     DGLGFRPAHLGPTVLRTETAALYAVAAIQVILRERSSWTPTA"
BASE COUNT         2355 a         4989 c         5357 g         2338 t
ORIGIN      
        1 actcggccgc ctcccgggtg gacaggtgct ccaggacgat gcggagacgg gggtagtcgt
       61 ccagcaggcc ccggaccacg ggcaggaagg cccgctcccg ttccaggacc ggggcggccg
      121 ggtcctcccc gtggatggac agcaccaatc cggcatcctc catggccgcc agggcctccc
      181 ggatctcccc gggttcccgg atcccgtcgc tcgaatgggt ggtcgcgcct gcgggatagt
      241 acttcccgac ccgggctccc gcgccggcca gggagcgcac ggtttccgtg tccatcccgg
      301 gcaggagctt gaagctgagg accaggtcca ggtcgggggc cgcggcacgg accgaggcgg
      361 cgtaggcccg gagcgtcgca gcgtccgcca ggggaggcag ggtgttgggc atgggcaggg
      421 cccggtcgaa gagggccgcc gtcctgcgga cgaacttctc caggccgggg ccggagcgga
      481 aatggacatg gaaatcgtcc ggggcccgga tgaccaggtc acgcatcgaa ccggaatccc
      541 accagggaaa cgtcgtcctc gaactgcccc cggccggagg ccgccctcac ggcccggacg
      601 atgccctcca ccccggcggt tcccaagctc cggttcgact cctccaggat ggaaaggaac
      661 ccggggagtc ccaggatgcg atcgtccgcg ccgcggacct cgtagatgcc gtcgctgaag
      721 aggtacagga cggaattcgg ggggatttcc gtctccgccc cggggaactc cgccgcgtcg
      781 tcgacgccca ccgcggggcc ttcggtcctg agttcccgga cgggttcgtc cggagccagg
      841 agggcggctg ggggggagcc cgcgctggcg taccggagcc tccgggtccg ggggtcccag
      901 agcccgaacc aggcggagaa gtacaggttg ttctgctcct ccccgcggta ggaggcgttg
      961 agatccgaaa ggatccgccg gggatcccac aattcgggtt ccgcgcggga cagggagttc
     1021 acccggtgga ggaggctgac ggacaggagg gccgcctcga tcccgtggcc gctcacgtcc
     1081 aggaggtaga gggctatcct gccgtcctcg agcctcgtgt acccgaaggc gtcccccccc
     1141 aggtcccggg agggcaggaa gacccagtcg gtccggagtt cccgccaccg gaccttccgg
     1201 ggaagcagcc cccggacgta ctccgcggcc ctccgaagct ccgcggccag gtggttctgg
     1261 gccgcagcca cggccctaag ggagagcctc tcccgggccc gcaggcggtt ccgctccagg
     1321 caggattcga tccgggcccg caggaccacg gggtcgaact cccggggaag gtagtcctcc
     1381 gcgcccaggc ggaggcagcg ggctacgctg ctggattcct ccagggcgga gatgacgatc
     1441 acgtggatgt cccgctggtt cggggccgcc ttgatccgtt ccaggaactg gtagccgttc
     1501 atcccgggca tcatgatgtc caggaggacc aggtcgtagg acccctgatc cagggcgttc
     1561 agggcctggg ccccgtccga ggcctggtgg accgagtggc cctggcgctc gaggtgccgg
     1621 gtgaggatct cccggttgaa ggcgtcgtcg tccaccacca ggatgctgcc cgccaggcgg
     1681 gaaggacccg cggattcctc gatcccggcc agggattcct cggacagttc ctccgcgtcg
     1741 aaggcgtggg ccagggcact ctcgaagagt tccgcggtcc ggttggatgc ctcgagtatc
     1801 ttctggaggt ccgggatgta cagggaaagg tcctcggatt ggcaggcccg cttggcggcc
     1861 tggatctggg ctatggcgtc gtacaggagc ccgtagatcc tccgcttcag gggctcccgg
     1921 ctctccgcgg ccggggctcc ctccccgggg gccgcgaaga actggagcag gggctcccgg
     1981 agggaaaggg ccttttcccg caggtctccg aaggcggttt ccagctcgga ttccctccgg
     2041 cccgccgcgt cctccagcag gatgtccgcg tatcccacga tgtggttgag tagggtgcgt
     2101 atctcgtggc gtatccgggt gcgggattcc ggggtcgtca tccggcttat gtccgctcgg
     2161 cgtgctcttc ggcgtacttg cggaactcct cctcggtcat cctgtccgcg tacaggggat
     2221 acagggcctc gaccagctct tccagggtcg ggaatttcgc tccgtccaac ttgaatgcca
     2281 tgctcgcgcc tttcctgtgc gtccggtgcc gatccatagg ggggcgggat caactatagc
     2341 cgatgggagg ggagggatca atcggtggac ggtggacagg gagggggggc gcctgttgcc
     2401 taatcgccct ccggaccgat agagtagccc catgtacaag taccgaatcg aggggggctt
     2461 ccccatcaag ggccggatcc gggccagcgg aaacaagaac gcagccctgc cctgcatcgc
     2521 cgccgccctg ctggcggaag agcccgtcat cctgcgcaac atcccggaga tcgaggacgc
     2581 ccaggtgatg ctcgagaccg cgtcccgcct gggcgcccgg gtggagaaac tggatcccgg
     2641 ggtctggagg atcgacgcct cgggacccct cggcaccgag atcccggagg agcagtcccg
     2701 gaagatccgg gcctccatcc tcttcgcggg tcccctcgtg gcccgcacgg gccgctgcgt
     2761 cctcccgccc cccgggggcg acgtgatcgg ccggcgccgg ctggacaccc acctcctggc
     2821 cctggaggaa ctgggcgccc ggatcgaggt ggacggcaag ttcgtcttca ccgccaacaa
     2881 gctggtcggg gcggacgtct tcctggacga ggcctccgtg accgccacgg agaacgccgt
     2941 gatggccgcc gtccgggcgg aaggccggac ggtcctgcgg aacacggcca gcgagcccca
     3001 cgtccaggac ctctgccgca tgctcgtggg catgggggcc tccatctccg gcatcggctc
     3061 caacatcctg gagatccagg gcgtgcccgc cctgggaggg acggatttct ccatcggccc
     3121 ggacttcatg gagatcggct ccttcatcgg tctggccgcg gtcacccacg gggagttgga
     3181 tatcgaggga gtgcggtcgg acgagctccg gatgatcaag ctgggcttcg ccaagctggg
     3241 gatacggtgg accgtggagg gcgacctcct tcgggtcccc gcgggccaag tcctcaaggt
     3301 cgtcccggac ctgggcggtc agatccccaa gatcgacgac gccccgtggc cgggtttccc
     3361 tccggacctg accagcatca tgaccgtggt ggccacccag gtggagggca ccagcctgat
     3421 ccacgagaag atgttcgagt cccggatgtt cttcgtggac aagctcatcg ggatgggtgc
     3481 ccggatcatc ctctgcgacc cccaccgggc cgtggtctcc gggccgtccc ggctctcggg
     3541 ctccgagctc gtctccccgg acgtgcgggc gggcatggcc atggtcatcg cggccctctg
     3601 cgccgagggg accagcatga tcaagaacgt gtaccagatc gagcggggct acgagcgcat
     3661 caccgagcgc ctgcgttctc tgggcgcccg gatcgaccgc atcgaggatt gttaagcccg
     3721 ggcccgggac aggcgctcgt aggcctcccg ggcctcccgg aacttctcct cgttcccggc
     3781 cggtccgtcg gggtggtgga ggcgggacag ccgcttccag gcccggcgga cctcgtccgt
     3841 cggcgagccc gggggaagtc ccagcacgtc ccaatcgtcc cggtagtccg ggaaatgccg
     3901 ggcccgggcg agccgcgcct cctcctccgg aacccccagg gctgccaggc gggaggaggt
     3961 gtccaggtcc tccccgggtc cgaggtcccg ggagttcatc ctccgcaggg cgaagaaggc
     4021 atcggccgcc aggggccgca gggcgcggtc cgcccggccg tcgaaggccg cggtgagcac
     4081 ggcgatgctt cggtccaggt ccccggaggg acggaccgcg gaggccgccc gggccgtcca
     4141 ggccgccagg ccccggggtg ccgaggcgga atcgcgcagg acctgtccgg cgctttccgg
     4201 gggtatcccg agcctgcggg cttccgcggc cgccaagccc gccaggaggg cggcccccgg
     4261 cgcgggctcc gaccggggcg ggggggagga gtcgggattc tccaggtagg aagccagttc
     4321 cgcgcgcagg cgctgggtcc ggagtatctc cgcccccatg acgcccagga gggctcccgc
     4381 gggcactccg tagaatcccg ccaggatacc cccggccagg gcccccagga gggccggcgt
     4441 tcccgcgcgg gccgcctcag tcgggcgcat gcacgccgag ccttcccacg gccaggaagg
     4501 acagcgccag cagggagacc aggaagccgg cccagacggc cagcgccccc gtggaaggca
     4561 ggtcggcgaa gaggcggccc aggaggtact gtcccgcccg gccggccgcg gccatcggaa
     4621 tcgaggccag ggccgtcagc aggggaaggg tcaacatcat ccggaggcgg ccgggttcct
     4681 cgtcccggcg gaatcgggcg cccagggcgg tgcccaggag taccagggtc atcagcatca
     4741 cggggatgga gatccggagg gcggtctcca tgcggtacgg ggacggatcg attccgtagg
     4801 ccggggcgat ccgggctccc gcggccaggc ggaagaaggg gatgcgctcc gcctggtcct
     4861 gaaggtccag caggatcgaa agggcctcga aatccatggg caggaccagg accgtggagt
     4921 ccggggggac catggagccc ttcaggaacc ggggccggga cgcctgacgg ggggagcccc
     4981 gttccaccat acggaggctg acggcgctgg ccgggggcgg cgcctccttt ccggggggtt
     5041 ccgaacccgc cgcgggcagg ccccggaggc gagcgtaggg ggcctggaca tggaggacgg
     5101 gtccgttctc gccgacggtc atgtactcga attcctccag gtacaggaag ggcccggcga
     5161 cggccacccg ggaggcgtac aggacatggt ccaccccttc ggacacgacc cggacggcca
     5221 ggccttcccg gctctgcccg gcgaaggccc gtcggaaatc ctccccgaag aaggccgtct
     5281 gggcgaggcg gtcgaggctt tccttgcggt agcgtacgac gtcggcgtcc ccgggatcct
     5341 cccgattcag gtccgtgaac gcccggtacg cctccaggta ggcctcggac tgaaggaggg
     5401 cgtaggcctc ggccttgcgc tcgtggaagg cggcccgggc cttgtccgcg gggttctccg
     5461 ggggggtggt gatccgttcc caggcgtccg cctggagttt ccgggcgtcc cgccgggagg
     5521 gatccaggat cagggacttg ccggcgtacc aatgggcgct gtagaagtcc cccctgcggt
     5581 agaaggccag ggcccgctgg tagaattcgt acgcggatgc cccgtcgtcc gggggggcgg
     5641 ggggagcttc cgttttccgg ggggaggtgt cctcggactg ccgggcccgc aggacggccc
     5701 gctggacctt ttcgttcaag agcttgtagg cggtctccgt cgagtcgatc gccccggcca
     5761 gggaagcccg ctcctgggct cgggccagat ccccgtctcc cagggccgtc tcggcgtccg
     5821 ccagggcgtc ccggaagagg gtggacaggg attcatacac ggtctgggcc cggcgcaccc
     5881 cgggcaggac cgtgagttcc agggcggaga agagcagggc caggatgagg gcggggacca
     5941 ggaccggccg caggagctcc gcgaagcgca gaccctgctc ccggtggagg ctcatggacg
     6001 ccgcgcaggc cgcgaagagc aggggaggca tccacgcggc caggagcgag gcggcccggg
     6061 ccagctccca tcggatcgcg aagcgggcga tgatgtcgga ggagcggaat tccaggaggg
     6121 cgaccacgaa ggccgcggcg aagcccagga ggacggcgac cgccacggcg gaccgggatt
     6181 cgctatcgcg gaccgccacg attcctccgg gccccccctc gggccgccga gaagggcctg
     6241 cacagggcga aatccaggaa caccagggct ataccgcagg cccccgccag gatgcctgca
     6301 cgcagggtcg ccggaacccg caggcccacc agggaagcca ggtctccggc ggcgggggac
     6361 cggaagatcc cgtggagggt gcccagcagg accaatcccg ccagggccgc gaaggtcccc
     6421 accaggggcc agcggaagag ccgggagagg ggccacagcc cgcagatgag ggcggcgacg
     6481 ccgagggcgg cggccagggc ctcgaacagg ttcccccggc ccagctccag gtaccaggtc
     6541 cggagttccc ggaaccccgg cagggggtcc gaggaggacc ccgggttccc gaggggcagg
     6601 ggggtccggt cggccatcag gattccccgg tcccgccggt aggttcccga ggccgcccag
     6661 gccaggcggg gtccctctcc ggaaggccgg acgtagatga ttccggtggc cgtgtccccg
     6721 gagctctccc ggacgaaatg ccgcgccggg gaggaggcgg ggacggacgc ggtcggaccc
     6781 gcgggaaagg gttcgggggg ggtcgtcagc gcccgggata cccacacccc gccggaaagg
     6841 acggcggcgg tcagtgtccc caggatcagg tacccgctca ggcggtgccg aagggtccgg
     6901 gtggccagga atcccgcgcc caggagggcg atcgagacgg atacgggcag ggattcgacg
     6961 gcgtaaccgg cccagaagct cagctggaag gagctcccgc cggaggcgct tttcatgagc
     7021 ggaccggccg ccaggatgag ggtttccacg atcacagcgc agaggaacca tgcaaggtat
     7081 cggcccgcga cggaaagccc tcgtttcatt ggagctaact ctaacacgcg cccatcccgt
     7141 tggcaagttc cgggagcata tagacaaacg gggtttcgag tccgggcccg cctcggtacc
     7201 aggcttgagg agtccacaag ttatccacag ggaaaaccgg aatcccgggg gatccccggc
     7261 ggcggttcgg cggatttgct ccacgcttgt accccgggat tttggagatt atcatgaaga
     7321 tgacaattaa atgtatatca tgaaagaaaa tagaatctag cgtaaaaaag taaaaaatac
     7381 acggttcctg tcgtctacat tattatacgg cctagggggg gggcatagga taaggaatta
     7441 tttctcaaca ggttatgcac agggtgttcc gtcgagccga tacccgtagt tcctgaccgc
     7501 cgcaaggaat gagcccgccc gggaatcccc ggcggcccgg gccagccggg accgcaaacg
     7561 gcagatcgtc atgtcgggtc ttcgggacgg cgagggtccc ggaccgtgtc ggacgcggag
     7621 gaaggccttg cggggaacgg gcctgccgag gctgtgaagc aggagctcca gcgcccggcg
     7681 ttccccttcc gggagggcca cggtgccggc cgctccggcc aggcgcccgg tcgaataggt
     7741 aaacgggtgc tcccccagcc ggaacctcca cgtaaggaat cgaagggccc gagcttcgag
     7801 ctcctccagg tcccagggct cccggaggta gtcggcgcaa ccctccgcga aggcgtccgc
     7861 cgtcaggctc gcgggtccgt aggcgatccg gagcacgtcc ctgtccgctc cggctccctc
     7921 caggaggagc cccgcgggaa ggacgatcag gtcgcagacg aggggggatc cgaggtcggg
     7981 ggaaaagaaa aaggagaggg gcccctcggc attcctctcc tcttcgacat gcttgagccc
     8041 ggggtcgatc accaggaccc gcacggccgc gtcagttctt ggtgcccgcg tcctcggtga
     8101 gcatcttggc cttgagctcc tccagcagga ggtccgcgct ggccccggag gattccagct
     8161 gggcgaactg cttctccagg gacgtgtcct tggacaggtc ctcgagctcc ttggccgctt
     8221 ccgccttggc ctccagctcg tccaccttct tggccatgcg gtcgaaggtg tcgaaggcgc
     8281 tggtgttgcc ggacatggtc gtcagggtct tctggatctt ctcctgggct tccgcacgct
     8341 tggcccgggc gatcaggatg tttttcttcc gggacgcctc gtcgatcttg ttctggagct
     8401 gccgcaggct ctccttgagc ttctcgaccg aggccttctg cgcttcccac tggggcttca
     8461 gctggaggaa atacccctcg tactcctgct tgcgcacgag ggcttccttg gccaggtcgt
     8521 cccggccggc gcggaccgcg agcatggcct tcttctccca ctcgtccgcc tgccgcttct
     8581 gctcggcgag ctcccgctcg atcttcttct cgtccgcgat ggccatggcc acggatttct
     8641 tggactccac catctgctgg ttcatgtcca ggagcagctg gttgagcatc ttttccggat
     8701 tttccgcctt ggcgatcatg tcgtttatat tggacgagac gacggtcttg aatcggctga
     8761 agatacccat cggggcctcc ttgttctctt ccgtcaggcc cggaatttac cgaggacggc
     8821 gtagtgctgg gctagggcga agccgaccgc gtcgagggcg gcttccagtt cggagcggtc
     8881 cagggtctcg agctccaggt tgtccaccag gacgatgtcg tccccttcca gggcgtaggc
     8941 tccgtgcagg aggtccgacg cgttcagccg gagcagggtt tcgaagagtt cctggcgatg
     9001 ttccttgggg gcggccatga ccttggtccg gagggccacc accggatccg cctgggtgat
     9061 gacgacctgg gtcagtcccc gggcctcgtc gttcacgagc caggcgttcg gggagatttc
     9121 ctcgtactgg atctcgaggc tgatcaggta gctttcaacg cggctgagtc cggccatcgg
     9181 ttcctccttg ggcggcgtag acgaatccgg cggaccacga tgcgggatca gcctaggaac
     9241 acctgccccg cgttgtcaat agccaggatg gtcttgcact cggagcagcg gaaccggccc
     9301 ggcttggccg ccttgagttt cttgttgcag atggggcagg agaagatctt ggggaacacg
     9361 ctctgggacg agccctggtc tccctggcgg aagaagccca tggcctcctc caggttgtcc
     9421 ttgatgttga agaactggga gaagccgagc agctggaaca cctcgtagac cttcggctgg
     9481 atctccagga ggacgatgtc cccgccccgg ggtttcacgg acttgaggaa ggccgtgaag
     9541 gatccgatgc ccgtcgagga gacgtagttg agcccgccgc agttgaagat gaggcgtacg
     9601 aagccggagt cgatggcctt gctgacccgt ttctggaaga agttggaatt gtaggtatcg
     9661 atataccccg tgaggaagag ggtgaggcag ttctcgaccg ggtcgacctt ctgcaggcgt
     9721 atctttagac tatcgtcctt ctcgtcgtcg aatcctggaa cgatatcgtt attattcatg
     9781 gtgctcctct cggatccaaa gatgaaggat acgccgtatg caggtattgt aggtccatcc
     9841 tagtatcaag tcaatctcct gtcaaatccg cggtcccggg cgaatccggg acttctagat
     9901 tttcggcatt gtcgaccaaa aaatccagag ccctcgagcg aaatatcgag gaatccgcat
     9961 accctcggtt ccagcccgtc tccttgattc taccacgccg ccaggccttc gtccgccgtc
    10021 aggatacccc cgttccagag gggcgggtcc cggcacagaa ggtccgcggc caggtccccg
    10081 aagacgcccg ggtcggtaag gctgcccccg ggggtcttcg cggccagtcc ctccagggtc
    10141 tccggggtca ggtactccgt ccggacgaat ccgggacaga ggaccagggc cgcgatcccg
    10201 tcccgggcgt actccagggc gatggacttg gccaggactc ccaggcccgt cttggcggcc
    10261 gcgtaggcgg cgttggtgcg gaagccccgg atcccgtccg tccgggtgcc tccgaggaag
    10321 aggatccggc cccagcgccg ggaccgcatc cccggcaggg cggcggatac cagggccccg
    10381 ggcagggcca ggttgaaggc gaccgccgcc tcccactcct ccggggcggt ctcgtggagg
    10441 ggtttctgca ggaagggccc ccaggccgcg accaggatgt ccgccgctcc cgcccggtcc
    10501 aggagggggc ccgtgcccgc cagggaatcc agggaaaagg ccgacccctc ggcctcgatg
    10561 cccgcctccc ggaactccgc caaggcggat tccagctttc ggccttcccg gccgtgcacg
    10621 tccaggcggg cgccccgaag gcccagggcc cgggctatgt gtcggcctat gccccccgtc
    10681 cctcccagga tgaaggcccg gcggccggcg aggggggggt ccgcattccg ttgcatgaat
    10741 acagtatacg gtggacggtg gacggtggac agtggacaat ggacagcgac gcttctcgca
    10801 gaggcacggg caggagtccg gattcgtctc cgtccaccgt ttaccgtcca cggtttgccg
    10861 tattacactc ccctcatgaa gtccccgccg gagttcgtgg tcgtcctctg ccgggcccgg
    10921 gaaccgggca acgtgggcgc cgcgtgccgg gccatggccg ccatgggact cggcagcctg
    10981 gtcctggcgg actgtccgga ccatcccccg gagcccgtca agacgttcgc cctggcggcc
    11041 tatcccctgt tcgagcgtgc ccggcgcttc ggcaccctcg aggatgccct tgcggactgc
    11101 gccttggccg cgggattcac ccgaaggacc ggacggctcc gggtacggga gccccgggac
    11161 gtgacggagt tcgcccggga cgcgatccgg cggtccggcc ggatcgccct ggtcttcggg
    11221 aacgaggcgg acggactttc cgcctcggaa ctggccctct gcgacctggc ggtgcgcatc
    11281 cccacctccg acgtcttccc ctccctgaac ctggcccagg cggtccagat cgcggcctgg
    11341 gagctggcgc gggcccgggc cgaaggaggg cgggtccctc cgtccgccga ggttcccggg
    11401 gatgccgacg ggcctgcggc cggaccggac gggacgtccg acgaggcgcc ggccgcccgg
    11461 gcggagatcc tggacgcggt cgaggccgcc gcggaggacc tggcccgggc cggattcttc
    11521 aagatctccg gccggccgga cttcgtgcgc gtcctgagat ccctgctggc ccgggcgggc
    11581 gcctcgggcc gggagctgga atacctgcgt cgtatcctgg gcaaggcggc ggccctgtcc
    11641 ggccggcggc cgaagccttg acccccggtc cggggggcgc tagagtaggt ttcggcccct
    11701 cgggctaagc ccggaaggag atggacgatg cgcagaccca aggccccttg gatcgcggcg
    11761 tgggcgatga ccttcgcggt cctgtccgcc ctcccaagtc ccgcccagtc cgggccgggg
    11821 ggtgccttcg acttcgcggc cagcgtggaa tccctggccc aggccgccga gaccggagcc
    11881 ggcctgcccg tcgggcgcta cgtcgtgctg acgggcaccg tgcgctccac ggtatcccag
    11941 gaggagggtt cgttctccgc ccgggtggag ctcgctgcgg gccaatggat cggcacctcc
    12001 cgcatcgtcc ttcgtaccgt gctggtcgag ttggccgggg attcgtaccg ggacctcttc
    12061 gaccgcggtt ccccgtcctt cctccgcccc ggatccgccg tgctggtcgt ggcccgggtg
    12121 accggaaccc gggaggcccc ggagggcgga cgggtggccg tgctggaagg gatccacctg
    12181 cgccgcctgg acaccctgta gaccgggacc ggcctgtccg ctattcggcc cggacccggg
    12241 gaagggcgga gggcctgcat ccgaaatcca catgctccgc ccggatgatg ccgccctccg
    12301 attccgaggc cgccaccgcg gcccggcaga tcgcgttcct gagctgccgg acgtttcccg
    12361 gccagtcgta gcccgagagc agttccatcg cgtccggccc gatctccgcc gctagtcccg
    12421 cctggtccag gaaatgccgc gccagcaggg ggatgtcccc gaggcgttcc cggagcggtg
    12481 gaagcttgag caccagggtc tccagccgtt ccaacaggtc gaagcgcatg ccctggaacc
    12541 ggtccagctc cacgttggtc gccgagatca gccggacgtc gcaggatgag gaggaccggg
    12601 ctcccaggcg gcggatctcg ccgttttcca ggatccggag cagcttggcc tgggccccgg
    12661 gcgagagttc cgttatctcg tccaggaaga gggttccccc gtcggcggtt tcgaaggtcc
    12721 cgggccggga gaccgcgccg gtgtaggctc cgcgctccgt cccgaaaagc tcgctctcca
    12781 ggatggattc cgggatggcc ccgcagttga ccgccaggaa aggccgggaa gcccgccggg
    12841 aaagaccgtg gatcgagcgg gccgccaggt ccttgccggt gccgctctcc ccggtgatca
    12901 ggaccgggaa gtcgtaccgg gcgaagagac ggatccggcg gcggaccccg cgtatgcagt
    12961 cgctttcccc caggaggatc cgggtatccg gatccggttc cccgtagggt cgggaaggtt
    13021 cccggcagga cggttccgtc gaccgggagg accggacccg ccggagccag gctttcaact
    13081 cgtccccggg gcagtcccgg gaaacgcagg ccgccgctcc ctccctcaga agcgcggtcg
    13141 tccgggcggg atccccggcg ggaagccggc agaccagggg cacggagggg aggcgggcct
    13201 tgaggagccg gatccttcct gcggcgtccc cggcgaccgg gtccccggct cccaggatga
    13261 ccaggtccca gccctcctcg gccgccgcgg cttcggcctc ggccggggtc ccgacgccgc
    13321 ggacgaggta ttcgtctgcg aggagggcgg cgaggatgtc ccgttcctct tcgtcttcct
    13381 cccagtggag aatcctgcgc atgcccaaac ggtagagcgg gcggatcgcg gcttcaatag
    13441 gccccgagcc cgggactctg tagattattg tctacatcca gaagcggtcg gaccgggggc
    13501 cggagcggcc cccccggcac ggggacgctt aaagccggcg gatcctgtac caaagcagga
    13561 ggaggcggta ccgtaggtag ggcaggatcc ggaagacctt ccacgggcgg cggagggtgt
    13621 agggaagcca ctccaacccg cggtcgaaga gggccttgga cggcttgggg accttttccg
    13681 cgaagacgtc gaagaggtcc gagcaccaga ggtagaggcc ggagttgaag tgcttgaggc
    13741 tccggggaat ccaccgctcc cgcccgatga ctccccggcc caccaggagc agggtgggcg
    13801 tggacttgcg gatggcctgg agtatgctct gttccatctg cttgggataa tggccgggaa
    13861 agcggcccac gatccggatg cccgggtagg tggcgtggat gttctcctcc gccaggctca
    13921 ggttgcgccg ggtggagccc aggaggtaaa cggaccggtt ccaccgttcc agggcattca
    13981 ggagccggat ggcgaaatcg aagggcatgt accggaccgg ttcttcccgg cggaggaacc
    14041 gggctccccg gaccagggac cgggagatgg gcaggaccag ggaggccccg ttgaccatgg
    14101 tccggaattc cccgctcctg cgggcccgga gcaggtccca gagggacaga aggacgatct
    14161 ggtggttcct tccgtcggag aacatggagc ggatgacgtc gtcgaagtcc tcgggcttga
    14221 ggatgtccag gggcaccttg aggagggaaa ttcgggttac gcggtcggag tccatgagga
    14281 acgctcccgt aggatcactt gaatggcggc cacggcatac agggcggcgg tctcggtccg
    14341 aagcaccgtg ggtcccaggt gggcgggccg gaagcccagg ccgtccagga agcgcacctc
    14401 atccatggaa aagccgcctt cggggccgac gcaggccacg atctcttcgg gttctccgga
    14461 aagatagcga tgaagggccg ctccttcaag gggcgcctcg tggaaataga ggcctattct
    14521 gcccggggcg gcctttccga ggatcccggg aagctcctgg aagggccggg ggctgtccac
    14581 ctcggtccgc agggaggatc cgctctgctg gagggcttcc cggacgatgc gttcccagcg
    14641 cgcccgcttg gccgccgccc cgccctcgtc ggagatccgg gccacggaac gggagctttc
    14701 gaccgggatg atccgcgcca ccccggcctc cgcggcctgg cggacgacca ggtccatctt
    14761 gcggcccttg ggcagggcct gcaccaggac gaggcgcggg agtctcgtac cccccgggcg
    14821 gccttctccg gtccgggtgt cggggcgcac ggccagttcg acccggcccg cctccacggc
    14881 ccggatctcc aactcgtagc ggcggccttc ccggtccagc cccggaaagc ggtcccccgg
    14941 ggacaggcga agcaccgaga gcaggtatcg ggcggcttcc cccgccagga cgcagccggg
    15001 ctccccggac cagtccgggg gtaggacgaa ttgacgcat
//
LOCUS       DAOTEI010000079        14823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2780_length_14823_cov_5.198131, whole genome shotgun sequence.
ACCESSION   DAOTEI010000079 DAOTEI010000000
VERSION     DAOTEI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2780_length_14823_cov_5.198131"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="P5313_09030"
     CDS             <1..1157
                     /locus_tag="P5313_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY80547.1"
                     /translation="IVDLGPGAGVDGGHVVAQGTPEEVMRSRKSLTGQYLAGSISIPV
                     PAERRAGNGNRITIRGCREHNLKGIDVSLPLGVFTVITGVSGSGKSTLLTDLLYPVIS
                     NAVMGTSWREGDYGEVEGLESIDKIINIDQSPIGRTPRSNPATYVGVFTHIRDLFATL
                     PESKARGWKPGRFSFNVKGGRCGNCQGDGTIRIEMNFLPDVYITCDACHGKRFNSDTL
                     DVRYKGKNISEVLDMTIEEAASFFEHIPPIAHRMNTLLSVGLGYIKLGQSALTLSGGE
                     AQRVKLAFELAKRSTGKTLYFMDEPTTGLHFADVKQLMEVIQRLVEAGNTVVMIEHNL
                     DVIKQADWVLDLGPEGGDKGGSLIARGTPEEIAKAKGSYTGKYLRESLKQ"
     gene            1265..4657
                     /locus_tag="P5313_09035"
     CDS             1265..4657
                     /locus_tag="P5313_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80548.1"
                     /translation="MSRSRTAWLPILLAALAAGAVSAQDDAEPEARVAETEAEPWAGA
                     EGTRALDIAGAGYYELIAWVRALGLPETGGADQLRSRLYSQYGIQAPAAGQAGDSIRI
                     EAADRTEYFKLDAPGEEYVRLSGGVVLTYTEGDSGTTHRIRADEILYNRTTNLLAARG
                     AVDYEKSGPSGTETFYGSSITVDLDSWEGRFLDGRSVRSSGEGAEESDLVFAADEIRK
                     LKGGVLSLRDGLITSSRAEDPYWSVRASRIWLLSENEWAIANAVLSVGNVPVLYLPFF
                     YYPGQEIVFHPAIGYRTREGRFLQTTTYLVGEKAESSGDDFNLFKTVRTGPGYEKELR
                     GVFLRETNRPRTEVPRDNLKLLADVYSNLGAFLGVEGTFQSLGGFRDLKFSLGLGLSR
                     SLFASGGYYTPFTSAGDFRSVWNESTLWGVVLPFRYSLNLDTKLALGPLTAAVTLPLA
                     SDPFFDQDFRNRSEDMNWLKLMSGDAPATATGETSSFTQRLSLSGAVPVPGLAPWIGA
                     LTLDRLTISLYWKPILRATPVDPDEATLFAVDPLRKFFAPELFTIADLALTIRGELLG
                     FSGTPSAAPDPARGSSAGAGAGTEAPASGLPEPEPPWAAEVPEPAGNDGAPSDDGRGG
                     DAVLGSSGPEDWTFKPPPASPDPAASRAAGLSGGLSYSISPSAQFERRYPTAFWDEPA
                     DVVNEGLYDLAGYRLAASLDARTSWASDVLSSSLTLAWNSQAQVRSGIDPDITHVSAA
                     QRDAWAVQDARYRYERVTGTLKLTSFPLREIWLLAPTSLSYTLTGYLYNTVYREGSAP
                     GVPVYDVFGPDWSTDTIKVHSLAATLGVRPYGYEQSLTLTTTLPPRLESYGLDLKANL
                     PYAIFQGTTKYYRPVAGADFLWDALTLRATLGPAEGPTLTDQFVWNTEQGYAASNTAS
                     FAWGGFRASLAARRSQAFELIPGSGWQAVGAEELRLSEAAASYRAEADLGLSRDGSVR
                     FKGSMNAQFSQSLLKATESVLALTYGLTLSVPGILDVEIAGTSQNSSVWRYAPDWFGM
                     SGLGFEPVNVLADLWNSVSLWDPAALRSAQFKLKGLSVKATHYMEDWDLVFEYKGGPE
                     LITTSTPYVYEFISSFSLFLTWKAIPQITTSVVKDKEGFRTK"
     gene            4740..5693
                     /locus_tag="P5313_09040"
     CDS             4740..5693
                     /locus_tag="P5313_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRY80549.1"
                     /translation="MESCIGIALEDTSLLADICGVNDYNLRVLERLMGGRVFSRGNEI
                     FLETSDTGDREVFRRLLDQLVESVEDGLPASPDLITALHATLTPEGASSCSDAFRDAC
                     VQIPGGFGKVFPRTPNQTLYLKGLATHDISFAVGPAGTGKTYLAVAWALRELLSKNRR
                     KLVLTRPVVEAGESLGYLPGDLGQKISPYLRPLYDSMEALVPYETVRRLEESRAIEIA
                     PLAYMRGRSLNHCVVILDEAQNTTKEQMKMFLTRLGEKSAAVITGDITQIDLPRKTDS
                     GLVHALELLAGLDGIFVCRMDGRDVVRNPLVKRIIQAYESE"
     gene            5680..7908
                     /locus_tag="P5313_09045"
     CDS             5680..7908
                     /locus_tag="P5313_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRY80550.1"
                     /translation="MNPSDPSGPAPFSWGKLRAALNSGREKRPEILSLAGAFLTCAAA
                     FLLLGEADLARLGRADIGLEVGRVADKDVIAERDYVYTDESATRLRVEAEKRLVPPIF
                     VVDDRIGSQALASFEAFRDGFLKLAAEDLPPETFRLRFQREWPDLLGLDEAQALAAYT
                     APGQAFSHAAQVLTSLFERGIVAMPEQGLGGFNPDTIEIRRWVKGRLEYEQIAADRVP
                     TLRIALGLARTALQAKNLPRPAYQAALRLTELYLKENASFDSVLSRGRLEAAERSVEP
                     VVRKIERGERIIRKGFIVTETDMERLQVIRESSRAPNWGPLLGGTALLGLILLFSILL
                     LSHPVSGIRLDRKHYLFTLGLGTLYFVLAVGLRRLFALDAPGSLAVFLPTGLFSMLAA
                     ILLGTRFAALSTIILGFLALLGSGFDSLSFAAASFAGLSGTFAARNAEKRIDLIRAGV
                     ILGFLQGAAGFMLSGLASTEILDAARRVAADFLNGFFCGVLTLGFLPLLEQALNSPTR
                     FRLMELSDMNAPILKRLLTVAPGTYSHSVTVAHLAESACREIGANALLARVGAYYHDI
                     GKIEQPEYFIENQGGGYNKHDKMNPRLSATVIRSHVKLGAERARSLGLPDEVVEIVAQ
                     HHGNGIITWFYDKALKADGEVRPDDFSYPGQPPASREAAVVMLADSVEAATRSLKKPT
                     LARLEQAIHDLIMDKFAQGQLARTNLTFRDLETIMNTFTRILAGHFHTRIEYPKARES
                     AR"
     gene            7905..8360
                     /gene="ybeY"
                     /locus_tag="P5313_09050"
     CDS             7905..8360
                     /gene="ybeY"
                     /locus_tag="P5313_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRY80551.1"
                     /translation="MNSIGISVEGVDPPEWMERAESFARTVLSELGKDGWELSILLCG
                     DSFIRGLNAQYRGKDVSTDVLSFEQGEWYVSGDGDRRFLAGDVVISLETLGRTCADLG
                     IPVNEELKRLILHGILHLSGYDHDESDPRGEMLDYQESLLKKFSEVHLL"
     gene            8357..9148
                     /locus_tag="P5313_09055"
     CDS             8357..9148
                     /locus_tag="P5313_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRY80552.1"
                     /translation="MKTSSLLERLFPQRRRSLSEEDSDEASTERKEMIRGVEELSETT
                     VKEVMVPRIDTVFLPVDADRDEVLETAITCGHSRIPVYRDTIDNVEGVLYVKDLLRTL
                     VRGADLDLTGIIRKPFFVPDSKRIDSLLREFKRRRVHIAVAIDEYGGVSGIVCMEDII
                     EEIVGDIQDEFDNEREDIVALGDGIWLCDARVNIQDLNEEIDASLPVEEFDTLGGFVF
                     DLFGKIPARYEKASWKAWDFVIQEMDGHRITSVKMVRRPEEPGEP"
     gene            9145..11220
                     /locus_tag="P5313_09060"
     CDS             9145..11220
                     /locus_tag="P5313_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY80553.1"
                     /translation="MTRRPIRPGAPLSAAPLLLAALILSSSVPSLSAQTGTDAPALVR
                     RAAEARLAEDWFEAVELYLAALQVNPAYTDAIQGLAESYYALEEYGQALTRVTEARRF
                     KGDDPALLSMEAFIRIGLGETAKASELFRAVLARLPNDLEARFGLALLDLRSGRSTDA
                     RARLSESLRISPRNARALLSLALISADAGRSRDAQTYIESALRYHSQDPRTQYVAARL
                     AARSGREREAVDRARSALELRPSYGEARRLLASLLLDSGDPGTAAQLMESAVARDRDD
                     ALAWYTLGVARSRQGQTDPGIYAFEASLSLRPDDEVARRALEDLVIDATPVEDPRRSR
                     VAAERFARGRQYESNREYERALAEYRRGLKVDPYSKPGRERYAELLRLRGKPSSYLAE
                     LEFLKSLDKADPAILDAAENYASALSGAVGRSWGLDQLLLGKRPYRIAFFSLPGQGPA
                     YHPGVGAVLARALRDETARSSRYAVSSETAAAASFADAFRSARDSGADWFGLVRTEET
                     ERDIRLSLEVYVARTGSPAASYSSLRSGNERVTRAVSHLAGLLESAPPLRGTLLRRNG
                     DKVLVDLGRNDGVAAGDTLLVVRKGGIAPSPQGIGIAYREADVTGEIEITRTDEEVSE
                     GMLRRSGYLDRSNLGDSVVRKPDPPKPETGDAAAGKTPPPGNPAPQTEIPSGEPVWPG
                     LFEEIRRLR"
     gene            11436..12803
                     /gene="gdhA"
                     /locus_tag="P5313_09065"
     CDS             11436..12803
                     /gene="gdhA"
                     /locus_tag="P5313_09065"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRY80554.1"
                     /translation="MSNKEGIVNAYIKEVLDGLKARYPWENEFLQAAEEVLESIEPLI
                     EAEPKYKAQRILERIIEPERSILFRVPWIDDKGEYHVNRGYRVQFNSAIGPYKGGIRF
                     HPKVQLSTLKFLGFEQTFKNSLTSLPMGGGKGGSDFDPNGKSDNEIMRFCQSFMTELF
                     RHIGPDTDVPAGDIGVGGREVGYMFGQYKRLANEFTGVLTGKGLTFGGSLIRPEATGF
                     GAVYFAEEMLKLKNDSLKGKVVSVSGFGNVAWGACIKASQLGAKVVTISGPDGYVYDP
                     DGIGTQEKWNYLEQMLVIDRNKVETYAKKFPNAKFFAGKKPWEQKVDIAMPCAIQNEL
                     NGEDAKTLLANGVKIVVEVSNMGCTPEAWKLFIEKKIPFAPGKAANAGGVATSGLEMS
                     QNSMRLAWSAEEVDRRLHEIMINIHRACVETAKQYGHEGNYVVGGNIAGFKKVADAMI
                     AQGLV"
     gene            complement(13158..>14823)
                     /locus_tag="P5313_09070"
     CDS             complement(13158..>14823)
                     /locus_tag="P5313_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY80555.1"
                     /translation="TVHEKILNASRDFITLVSSEYVYEFVNESYCREIGLDRQAILGR
                     TVAEVWGKRKFETRIKRHLDECFGGAESHDIDRFKFGDEEKYVHVAYYPFREGTRVTH
                     VMVDTHDISALKDLQTRIINFEYKDSTTGLFNRRSFDIVLDMELEKARRSQTDKVRAI
                     LFVNLRNFTQINARYGYEVGDLLLEATGMRIREALRASDYVFRFEGKDLAIILTTMKR
                     STDLSIVADNIRGKAFYPYTHQGAVINIGCNIGASVFPDDGDDKKSLVKFALSAMEEA
                     QTRNEPLIIFNQELHRAALRKAKLRSDLRTALVAEQFETHFQPIVDAAGSLIGAEALI
                     RWSHPEMGNIPPMEFIPLAEESGDTIMIGRWVLFQVCKHIKRWSGLLGDRYISVNLSA
                     KEFAGEGLVEYIDRVLRSEGVPPAQLKLEITETQSMMDIEDAIGKIRRLKDIGVDVYI
                     DDFGAGYSSLAYLKRLPAGVIKIDKCFVDNVAEDEEERAFLAGMIGMIVSKDKRIVVE
                     GVATREQRDILAGLGVERMQGYYFSKALPAPDFEVLLGRREPLPVS"
BASE COUNT         2471 a         5065 c         4830 g         2457 t
ORIGIN      
        1 ggatcgtcga cctcgggccc ggcgccgggg tcgacggcgg ccacgtcgtg gcccagggca
       61 cgccggaaga ggtcatgcgg agcaggaaga gcctcacggg gcagtacctc gccggcagca
      121 tctcgatacc cgtgcccgcc gagaggcgcg ccggcaacgg gaatcgcata accatccgcg
      181 gctgccgcga gcacaacctc aagggcatcg acgtctccct tccgctcggc gtcttcacgg
      241 tcatcaccgg ggtctccggc tccggcaagt cgaccctcct caccgacctc ctctatcccg
      301 tcatctcgaa cgccgtgatg gggacctcgt ggcgcgaggg ggactacggc gaggtggagg
      361 gcctcgagag catcgacaag atcatcaaca tcgaccagag cccgatcggc cggacgcccc
      421 gctcgaaccc ggccacctac gtcggggtct tcacccacat ccgggacctc ttcgccaccc
      481 tgccggaatc caaggcccgg ggctggaagc ccggacgctt cagcttcaac gtgaagggcg
      541 gccgctgcgg gaactgccag ggggacggca ccatccggat cgagatgaac ttcctgcccg
      601 acgtctacat cacctgcgac gcctgccacg ggaagcgctt caactcggac accctggacg
      661 tccgctacaa gggaaagaac atctccgagg tcctggacat gaccatcgag gaggcggcct
      721 ccttcttcga gcacatcccc ccgatcgccc accgcatgaa caccctcctg tccgtgggac
      781 tgggctacat caagctcggg cagtcggccc tgaccctctc gggcggcgaa gcccagcggg
      841 tcaagctggc cttcgagctg gccaagcggt ccacgggcaa gaccctctac ttcatggacg
      901 agccgaccac ggggctccac ttcgcggacg taaagcagct catggaggtc atccagcgcc
      961 tggtggaagc cgggaacacg gtggtgatga tcgagcacaa cctggacgtg atcaagcagg
     1021 cggactgggt cctggacctg gggcccgagg gcggggacaa gggcggatcc ctgatcgccc
     1081 ggggaacccc cgaggagatc gccaaggcga agggctcgta cacggggaag tacctgaggg
     1141 agagcctgaa gcagtaaacg gcggacagtg gacggaaagc gagcggatac accggattct
     1201 tccgtccact gaccacggac caccgctgtt gcccgatccc cgactcctgc tttacactgc
     1261 ctccgtgagt cgatcccgga cggcctggct ccccatcctc ctcgcggcgc tcgcggccgg
     1321 ggccgtgtcc gcccaggacg acgccgagcc ggaggcccgt gtcgcggaga ccgaggcgga
     1381 gccctgggcg ggagccgagg ggacccgggc cctggacatc gcgggggccg ggtactacga
     1441 actgatcgcc tgggtccggg ccctgggcct tccggagacg gggggagccg accagctgcg
     1501 ctcccggctg tactcccagt acgggatcca ggcccccgcc gccggccagg cgggggactc
     1561 catccgcatc gaggccgcgg accggacgga gtacttcaag ctggacgccc cgggggagga
     1621 gtacgtacgg ctctcgggcg gggtggtcct gacctacacg gaaggggatt ccggcaccac
     1681 ccaccgtatc cgggcggacg agatcctgta caaccggacg acgaacctcc tggcggcccg
     1741 gggagccgtg gactacgaga agagcggccc ctccggcacg gaaaccttct acggctcctc
     1801 catcaccgtc gacctcgact cctgggaagg gcgcttcctg gacggacgca gcgtccggtc
     1861 ctccggggag ggcgccgagg aaagcgatct cgtgttcgcg gccgacgaga tccgcaagct
     1921 caagggcggg gtcctgtccc tccgggacgg gctcatcacc tccagccggg cggaggatcc
     1981 gtattggtcc gtccgggcca gccggatctg gctactttcg gagaacgagt gggccatagc
     2041 caacgcggtt ctctcggtcg gaaacgtccc cgtcctgtac cttcccttct tctactaccc
     2101 cgggcaggag atcgtcttcc accccgccat cgggtaccgg acccgggagg ggcgattcct
     2161 ccagaccacg acctacctgg tcggggagaa ggcggaatcc tccggggacg acttcaacct
     2221 cttcaagacg gtccgcacgg gcccggggta cgagaaggag ctccgggggg tcttcctccg
     2281 ggagacgaac cggcctcgga cggaggtgcc ccgggacaac ctcaagctcc tggcggacgt
     2341 ctactccaac ctcggggcct tcctgggcgt ggagggcacc ttccagagcc tcgggggctt
     2401 ccgggacctc aagttctccc tggggctcgg cctgagccgg tcccttttcg cctcgggcgg
     2461 gtattacacg cccttcacgt ccgccgggga tttccggagc gtctggaacg aatccaccct
     2521 atggggtgtc gtcctgccgt tccggtactc gttgaacctg gacacgaagc tcgccctcgg
     2581 gccccttacg gcggccgtga ccttgcccct agcctcggat cccttcttcg accaggactt
     2641 ccggaaccgg tccgaggaca tgaactggct caagctcatg tccggggatg ccccggcgac
     2701 ggcgaccggg gagacgagct ccttcaccca gcgcctttcc ctgtccgggg ccgtccccgt
     2761 ccccggcctg gcgccctgga tcggagccct gaccctggac aggctgacga tttccctgta
     2821 ctggaagccg atccttcggg cgaccccggt ggacccggac gaagccaccc tcttcgccgt
     2881 ggatcccctg cggaagttct tcgccccgga actcttcacg atcgcggacc tggccctgac
     2941 catccggggg gagttgctgg ggttctcggg caccccgtcc gcggcgcccg atcccgcccg
     3001 ggggagctcg gcgggcgccg gggcggggac cgaagctccc gcgtccggtc tccccgagcc
     3061 ggagccgccc tgggccgcgg aggttcccga gccggccggt aacgacgggg cgccctcgga
     3121 cgacgggagg ggcggggatg ccgtcctcgg gtcctccgga ccggaggatt ggaccttcaa
     3181 gcctccgccg gcctccccgg atcccgcggc atcccgggcc gcgggcctgt ccggcgggct
     3241 ctcgtactcg atctccccct ccgcgcagtt cgagcggcgg tacccgacgg ccttctggga
     3301 cgagcccgcg gacgtggtga acgagggcct ctacgacctg gccggctacc gcctggccgc
     3361 gagcctggat gcccggacgt cctgggcctc ggatgtcctg agctccagcc tgaccctggc
     3421 ctggaactcc caggcccagg tccgctcggg gatcgacccg gacatcaccc acgtgagcgc
     3481 agcccaacgg gacgcctggg cggtccagga tgcccggtac cgttacgagc gcgtcaccgg
     3541 cactctcaag ctgacgagct tccccctgcg ggagatctgg ctcctggccc cgacctccct
     3601 gagctacacc ctgacgggat acctgtacaa taccgtctat cgggaggggt ccgcgcccgg
     3661 cgttcccgtc tacgatgtct tcgggccgga ctggtcgacg gatacgatca aggtccacag
     3721 cctggccgcc accctggggg tccggcccta cgggtacgag cagagcctga ccctcacgac
     3781 caccctgcct ccccgcctgg aatcctacgg tctggacctc aaggcgaacc tgccgtacgc
     3841 gatcttccaa ggcacgacca agtactaccg gccggtggcg ggcgcggatt tcctctggga
     3901 cgccctgacc ctgcgggcca ccctgggtcc ggccgagggg ccgaccctga cggaccagtt
     3961 cgtctggaac accgagcagg gatatgccgc gtcgaatacg gcctccttcg cctggggcgg
     4021 cttccgggct tccctcgcgg cacgaagatc ccaggccttc gagctgatcc cgggctccgg
     4081 gtggcaggcg gtcggcgcgg aggaactccg tctctcggaa gccgccgcct cctatcgggc
     4141 ggaggcggac ctgggactgt cccgggacgg atcggtccga ttcaagggtt cgatgaacgc
     4201 ccagttctcc caaagcctgc tgaaagccac ggaatcggtc ctggccctca cctacggcct
     4261 aaccctctcg gtccccggga tcctggacgt cgagatcgcc gggacctccc aaaattcctc
     4321 ggtgtggagg tacgcgccgg actggttcgg catgtccggc ctgggcttcg aacccgtgaa
     4381 cgtccttgcg gatctttgga attccgtctc cctctgggat cccgcggctc ttcggagcgc
     4441 ccagttcaag ctcaaggggc tctcggtcaa ggcgacccat tacatggagg attgggacct
     4501 ggtcttcgag tacaagggcg gcccggagct catcaccacc tcgacgccct acgtctacga
     4561 gttcatttcc tctttttccc tgttcctgac ctggaaggcc atcccccaga tcaccacctc
     4621 cgtggtcaaa gacaaggaag ggttccgcac caagtagtga gaccgcacga accgggaccg
     4681 gccaggcgct ttcttgattt ttcagacccg gaagggtagg atgaggggaa gaggcgctat
     4741 tggaaagctg catcgggatc gcgctggagg ataccagcct gttggccgac atctgcgggg
     4801 tcaacgatta caaccttcgg gttttggaac gcctgatggg cggccgggtc ttttcccggg
     4861 gaaacgagat ctttctggaa acctcggata ccggggaccg ggaggtgttt cgccgactcc
     4921 tggaccagct cgtggaatcc gtggaggacg gtctgcccgc ctctcccgat ctgatcaccg
     4981 ccctccacgc caccctgacc cccgaggggg cctcctcctg ctcggacgcc ttccgggacg
     5041 cctgcgtcca gattcccggg ggattcggca aggtttttcc ccgcaccccc aaccagaccc
     5101 tctacctcaa gggtctggcc acccacgaca tctccttcgc ggtgggcccc gcgggtacgg
     5161 gaaagaccta cctggccgta gcctgggccc tccgggagct actgtccaag aaccggcgca
     5221 agctcgtcct gacccggccc gtggtggaag ccggtgagag tctgggatac cttcccggcg
     5281 atctggggca gaagatcagc ccctacctac ggcccctgta cgattcgatg gaagccctgg
     5341 tgccctacga gaccgtccgc aggctggagg aaagccgggc catcgagatc gcgcccctgg
     5401 cctacatgcg cggccggagc ctaaaccact gcgtcgtgat cctggacgag gcccagaaca
     5461 ccacgaagga gcagatgaag atgttcctca cccgcctggg ggaaaagtcc gccgcggtca
     5521 tcaccgggga catcacccag atcgacctcc cccgcaagac cgattccggc ctggtccacg
     5581 ccctggaact cctggccggc ctggacggca tcttcgtgtg ccgcatggac ggccgggatg
     5641 tcgtacgcaa ccccctggtg aaacggatca tacaagccta tgaatccgag tgatccctcc
     5701 gggccggccc cgttttcctg ggggaagctg cgggccgcgt tgaattccgg ccgggagaag
     5761 cgcccggaga tactcagcct cgccggggct ttcctgacct gcgcggcggc cttcctcctc
     5821 ctcggggagg cggacctcgc gcggctgggc cgggcggaca tcgggctgga ggtcgggagg
     5881 gtcgcggaca aggacgtcat cgcggaacgg gattacgtgt acaccgacga atccgcgacc
     5941 cggctcaggg tggaggcgga gaagcgcctc gtgcccccga tcttcgtcgt ggacgaccgg
     6001 atcggatccc aggccctggc gtccttcgag gccttccggg acggcttcct gaagctggcc
     6061 gccgaggacc ttcccccgga gaccttccgc ctgcgcttcc agagggaatg gccggacctc
     6121 ctcggcctgg acgaggccca ggccctggcc gcctatacgg ctccgggcca ggccttttcc
     6181 catgccgccc aggtgctgac ctccctgttc gagcgcggga tcgtggccat gccggagcag
     6241 ggcctggggg ggttcaaccc ggacaccatc gagatccgac gctgggtgaa aggaaggctg
     6301 gagtacgagc agatcgccgc ggaccgggtc ccgaccctgc ggatcgccct gggcctggcc
     6361 cggacggccc tgcaggccaa gaacctcccg cgtcccgcgt accaggccgc cctccggctg
     6421 acggagctct acctcaagga gaacgcctcc ttcgattccg tgcttagccg gggccgcctg
     6481 gaggccgcgg agcgctcggt ggagcccgtg gtgcgcaaga tcgagcgggg agagcggatc
     6541 atccgcaagg gcttcatcgt gacggaaacg gacatggagc ggctgcaggt gatccgggaa
     6601 tcctcccgcg cgccgaactg gggccccctc ctgggcggga cggccctcct cggactcatc
     6661 ctcctgttct cgatcctcct tctgtcccac ccggtttccg ggatacggct ggaccgcaag
     6721 cattacctgt tcaccctggg cctcgggacc ctgtatttcg tgctggccgt aggcctcagg
     6781 cggctctttg ccctggacgc cccgggctcc ctggccgtct tcctgcccac ggggctcttc
     6841 tccatgctgg ccgccatcct cctgggcacg aggttcgccg ccctgtcgac gatcatcctg
     6901 ggcttcctgg cccttctggg gtcgggcttc gactccctga gcttcgcggc cgcctccttc
     6961 gcggggctct cgggcacctt cgccgcccgg aacgcggaaa agcgcatcga cctgatccgg
     7021 gcgggagtga tcctgggctt cctgcagggg gctgcggggt tcatgctctc ggggctcgcc
     7081 tccacggaga tcctggacgc cgcgcggcgg gtggccgccg acttcctgaa cgggttcttt
     7141 tgcggggtcc tcaccctggg gttcctgccc ctcctggagc aggccctgaa ctcccccacc
     7201 cggttccggc tcatggagct ctcggacatg aacgccccga tcctgaagcg cctgctgacc
     7261 gtggccccgg gaacctacag ccactccgtg accgtggcgc acctggcgga gagcgcctgt
     7321 cgggagatcg gcgccaacgc cctcctggcc cgggtcggag cctactatca cgacatcggg
     7381 aagatcgagc agcccgagta cttcatcgag aaccaggggg gcgggtacaa caagcacgac
     7441 aagatgaacc cccgcctgtc cgccacggtg atccgcagcc acgtcaagct cggggcggag
     7501 cgggcgcggt ccctggggct gccggacgag gtggtggaga tcgtggccca gcaccacggc
     7561 aacgggatca tcacctggtt ctacgacaag gccctgaagg cggacggcga ggtccgcccg
     7621 gacgattttt cctatcccgg ccagcccccc gcctcccggg aggccgcggt ggtgatgctg
     7681 gcggattccg tggaagccgc cacccggagc ctcaagaaac cgaccctggc ccggctggag
     7741 caggccatcc acgacctgat catggacaag ttcgcccagg gccagctggc ccggacgaac
     7801 ctgaccttcc gggacctgga gaccatcatg aacaccttca cccggatcct ggcgggccac
     7861 ttccacaccc ggatcgagta tcccaaggca cgggaatccg cccgatgaac tcgatcggga
     7921 tctccgtgga gggcgtggac cccccggaat ggatggaaag ggcggaatcc ttcgcccgga
     7981 ccgtcctttc ggaactcggg aaggacgggt gggaactctc catcctcctc tgcggggact
     8041 ccttcatccg cggcctgaac gcccagtacc ggggaaagga cgtctccacg gacgtgctct
     8101 ccttcgagca gggcgagtgg tacgtgtccg gggatggaga ccgccgcttc ctggcggggg
     8161 acgtcgtgat ctccctggaa accctgggaa gaacctgtgc ggatctcgga attcccgtga
     8221 acgaggagct caagcggctc attctccatg gtatactgca cctgtcgggg tacgaccacg
     8281 acgaatcaga tccccggggg gagatgctgg actatcagga atcccttcta aaaaagttct
     8341 cggaggtaca cctgctttga agacttcctc gctcttggaa cggctctttc cccagcgccg
     8401 gcgttccctg tcggaggagg attccgacga ggcctcgacg gaacgcaagg agatgattcg
     8461 gggcgtcgag gagctctcgg agaccaccgt caaggaggtg atggtcccgc ggatcgacac
     8521 ggtcttcctg cccgtggacg cggaccggga cgaggtcctg gaaaccgcga tcacctgcgg
     8581 acattcccgg ataccggtgt accgggacac gatcgacaac gtggagggtg tcctctacgt
     8641 gaaggacctc ctgcgcaccc tggtccgggg cgctgacctg gacctgaccg ggatcatccg
     8701 caaacccttc ttcgtgcccg attccaagcg catcgattcc ctcctccggg aattcaagcg
     8761 gcgccgggtc cacatagccg tggccatcga cgagtacggc ggggtgtccg ggatcgtctg
     8821 catggaggac atcatcgagg agatagtcgg ggacatccag gacgagttcg acaacgagcg
     8881 ggaggacatc gtcgccctgg gggacgggat ctggctctgc gacgcccggg tgaacatcca
     8941 ggacctgaac gaggagatcg acgcctccct gcccgtggag gagttcgaca ccctcggggg
     9001 cttcgtgttc gacctcttcg gcaagatccc ggcccggtac gagaaggctt cctggaaggc
     9061 atgggacttc gtgatccagg agatggacgg ccaccgcatc acctccgtga agatggtgcg
     9121 caggcccgag gaacccgggg agccatgacg cgcaggccga tccgtccggg agcgcccctg
     9181 tccgccgccc ccctcctgct ggcggccctg atcctctcgt cgtccgtccc gtccctttcc
     9241 gcccagaccg ggacggacgc tcccgccctg gtccggcgcg ccgcggaagc ccggctggcg
     9301 gaggattggt tcgaggccgt cgagctctac ctggccgccc tgcaggtaaa tcccgcgtac
     9361 accgacgcta tccaggggct cgcggagtcc tactacgccc tggaggagta cggccaggcc
     9421 ctgacccggg tcacggaagc ccggaggttc aagggggacg acccggccct cctgtccatg
     9481 gaggccttca tccgcatcgg gctgggggag acggccaagg cctcggagct attccgggcc
     9541 gtcctggccc ggcttccgaa cgacctggag gcgcgattcg ggctcgccct gctggacctg
     9601 aggtccgggc gatccacgga cgcccgtgcg cggctctccg agagcctgcg tatctctccc
     9661 cggaacgccc gggccctcct gtccctggcc ctgatctccg cggacgcggg ccggtcccgg
     9721 gacgcccaga cctacatcga gagcgccctg cgctatcact cccaggaccc ccgtacccag
     9781 tacgtggcgg cccggctggc ggcccggagc ggccgcgagc gggaggcggt ggaccgggcg
     9841 cggtcggccc tggagctccg tccgtcctac ggcgaggccc ggcgtctcct ggcctccctg
     9901 ttgctggatt ccggagaccc cgggaccgcg gcccagctca tggaatcggc ggtggcacgg
     9961 gaccgggacg acgccctggc ctggtacacc ctgggggtgg cccgctcccg gcagggacag
    10021 acggatccgg ggatctacgc cttcgaggcc tccctgtccc tgcgcccgga cgacgaggtc
    10081 gcgcgccggg ccctggagga cctggtgatc gacgccaccc cggtcgaaga cccccgtcgc
    10141 tcccgggtgg ccgcggagcg cttcgcccgg ggccggcaat acgaatccaa ccgggaatac
    10201 gagcgggccc tggcggagta ccggcggggc ctcaaggtgg atccctactc caagccgggc
    10261 cgggaacgct acgcggagct tctcaggctt aggggaaagc cttcctccta tctggcggaa
    10321 ctggagttcc tgaagtccct ggacaaggcg gatccggcca tcctggacgc cgcggagaac
    10381 tacgccagcg ccctgtcggg tgcggtcggc cgttcctggg ggctggacca gctccttctg
    10441 ggtaagcgtc cctaccggat cgctttcttc tccctccccg gacagggccc ggcctatcat
    10501 cccggggtcg gggcggtcct cgcccgagcc ctgagggacg agacggcccg atcctcccgg
    10561 tacgcggttt cctccgagac cgcggccgcc gcctccttcg cggacgcctt ccgctcggcc
    10621 cgggattccg gggcggattg gttcgggttg gtccggacgg aggagaccga gagggacatc
    10681 cggctatccc tggaagtcta cgtggcccgg accggaagcc ccgcggcctc ctattcctcc
    10741 ctccggagcg ggaacgaacg ggtcacccgg gcggtatccc acctggccgg cctcctggaa
    10801 tccgccccac ccctccgggg caccctcctg cgcaggaacg gggacaaggt cctcgtggac
    10861 ctggggagga acgacggcgt ggcggcgggg gatacgcttc tggtcgtccg gaagggcggc
    10921 atcgccccga gcccccaggg gatcgggatc gcgtaccggg aagccgacgt gaccggggag
    10981 atcgagatca cccggacgga cgaggaggtt tccgagggga tgctccgcag gtcgggatac
    11041 ctggaccgct ccaatctcgg ggattcggtg gtacggaaac ccgaccctcc gaagcccgag
    11101 accggggacg ccgcggcggg caagactccg cctcccggga acccggcgcc ccagaccgag
    11161 atcccgtcgg gggaacccgt ctggccgggc ctcttcgagg agatacggcg actccgctga
    11221 ttggctttta atttatagat atatgtatac ttataccatc cccgggagat gctccgggac
    11281 ggaccctgcc ggagtgaggg aagatgtata aatatccgtt actacggaaa gtatttcatt
    11341 ctatgacaag attttcctgc cttgcccaaa tccggaaacc cgggtaagat aaacccagga
    11401 agggccgcag cgcccttcaa tcaccaaaca cgcgtatgtc caacaaggag ggcatcgtga
    11461 acgcatatat caaggaagtc ctggacggcc tcaaggcccg ctacccctgg gagaacgagt
    11521 tcctccaggc cgcggaggag gtgctggagt ccatcgagcc cctcatcgag gcggagccca
    11581 agtacaaggc ccagcgcatc ctggagcgca tcatcgagcc cgagcgctcc atcctcttcc
    11641 gcgttccgtg gatcgacgac aagggcgaat accacgtcaa ccggggctac cgcgttcagt
    11701 tcaactccgc catcggtccc tacaagggcg ggatccgctt ccacccgaag gtccagctct
    11761 ccaccctcaa gttcctgggc ttcgagcaga ccttcaagaa ctccctgacc agcctgccca
    11821 tgggcggcgg caagggcggc tcggacttcg atcccaacgg caagagcgac aacgagatca
    11881 tgcgcttctg ccagagcttc atgaccgagc tcttccgcca catcggcccg gacacggacg
    11941 tgccggcggg cgacatcggc gtgggcggcc gcgaggtcgg gtacatgttc ggccagtaca
    12001 agcgcttggc caacgagttc accggcgtcc tcacgggcaa gggactgacc ttcggcggct
    12061 ccctcatccg cccggaggcc acgggcttcg gcgccgtcta cttcgccgag gagatgctca
    12121 agctgaagaa cgattccctg aagggcaagg tcgtctccgt gtccggcttc ggcaacgtag
    12181 cctggggcgc ctgcatcaag gcctcccagc tgggcgccaa ggtcgtcacc atctccggtc
    12241 cggacggtta cgtctacgac ccggacggga tcgggaccca ggagaagtgg aactacctgg
    12301 agcagatgct ggtcatcgac cggaacaagg tggagaccta cgccaagaag ttccccaacg
    12361 ccaagttctt cgcgggcaag aagccctggg agcagaaggt cgacatcgcc atgccctgcg
    12421 ccatccagaa cgagctcaac ggcgaggacg cgaagaccct cctcgcgaac ggcgtcaaga
    12481 tcgtcgtcga ggtctccaac atgggctgca cccccgaggc ctggaagctc ttcatcgaga
    12541 agaagatccc cttcgctccc ggcaaggcgg cgaacgcggg cggcgtggcc acctcgggcc
    12601 tggagatgag ccagaactcc atgcgcctgg cctggagcgc cgaggaagtg gacaggcggc
    12661 tccacgagat catgatcaac atccaccgcg cctgcgtgga gaccgccaag cagtacggcc
    12721 acgaggggaa ctacgtcgtg ggcgggaaca tcgcgggctt caagaaggtc gccgacgcca
    12781 tgattgccca gggcttggta taaaccggcc tgcagggtca tggcgtcggg acgccatgat
    12841 cgcgcaggga ctggtctagc cgcggcgctt cgcgccgcgg ctgttcggaa cccgcgcaaa
    12901 gcgcgggttc cggctgccca ggatttggtc ctgtaggcgc ttcgcgccgt tgctgttcgg
    12961 aatccgcgct tggcgcggat tccggctgcc caggatttgg tcctgtaggc gcttcgcgcc
    13021 gcggctgttc ggaacccgcg caaagcgcgg gttccggctg cccaggatat ggacgaggaa
    13081 gctcgctgcg ccggacaggc tgatgatatg aagtatgaat aaacgtacgg gcggtcccga
    13141 aagggaccgc ccctttttta tgacaccggc aggggctccc tgcggcccag gaggacctcg
    13201 aaatccgggg cgggaagggc cttgctgaag tagtacccct gcatccgttc cactcccagg
    13261 cctgccagga tgtcgcgctg ttcccgggtg gcgaccccct cgacgacgat gcgcttgtcc
    13321 ttgcttacga tcatcccgat cattcccgcc aggaaagccc gttcctcctc gtcctcggcc
    13381 acgttgtcca cgaagcactt gtcgatcttg atgacccccg ccggaagccg cttcaggtag
    13441 gccagggacg agtagccggc tccgaagtcg tcgatgtaga cgtccacccc gatgtccttc
    13501 aggcggcgta tcttgccgat ggcgtcctcg atgtccatca tgctctgggt ttccgtgatc
    13561 tccagcttca gctgggccgg cggaactccc tcggagcgga ggacccggtc gatgtactcc
    13621 acgaggcctt ccccggcgaa ttccttggcc gagaggttca cggagatgta ccggtccccc
    13681 aggagcccgc tccagcgctt gatatgcttg cagacctgga agaggaccca gcggccgatc
    13741 atgatggtgt ccccggattc ctccgccagg gggatgaact ccatgggcgg gatgttcccc
    13801 atctccgggt gggaccaccg gatcagggcc tccgcaccga tcagggatcc cgcggcgtcc
    13861 acgatgggct ggaagtgggt ctcgaactgc tccgccacca gggccgtccg gaggtcggac
    13921 ctcagcttgg ctttccggag ggcggcccgg tgcagctcct ggttgaagat gatcaggggc
    13981 tcgttcctgg tctgggcctc ctccatggcg gacagggcga acttgaccag gctcttcttg
    14041 tcgtccccgt catccgggaa aaccgaggcc ccgatgttgc acccgatgtt gatgaccgcg
    14101 ccctggtggg tgtaggggta gaaggccttg ccccggatgt tgtcggccac gatggacagg
    14161 tccgtgctgc gcttcatcgt ggtcaggatg atggccaggt ccttgccctc gaagcggaag
    14221 acgtagtccg aggcccgcag ggcttcccgg atccgcatgc ccgtggcttc cagcagaaga
    14281 tcgccgacct cgtagccgta ccgggcgttg atctgggtga aattcctgag gttcacgaag
    14341 aggatggccc gaaccttgtc ggtctgggat ctccgggcct tctccagttc catgtccagg
    14401 acgatgtcga aggacctgcg gttgaacagc cccgtggtgg agtccttgta ctcgaagttg
    14461 atgatccggg tctggaggtc cttcagggcg gagatgtcgt gggtgtccac catgacgtgg
    14521 gtgacccggg tgccctcccg gaagggatag tacgccacgt ggacgtactt ctcctcgtcc
    14581 ccgaacttga accggtcgat gtcgtgggat tcggcgccgc cgaagcattc gtccaggtgc
    14641 cgcttgatcc gggtctcgaa cttgcgcttg ccccagacct ccgccaccgt ccttcccagg
    14701 atcgcctgcc ggtccagacc gatctcccgg cagtaggact cgttcacgaa ctcgtacacg
    14761 tactccgagg agaccagggt gatgaaatcc cgggaggcgt tcaggatctt ttcgtgcacc
    14821 gtg
//
LOCUS       DAOTEI010000080        14765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2795_length_14765_cov_5.037322, whole genome shotgun sequence.
ACCESSION   DAOTEI010000080 DAOTEI010000000
VERSION     DAOTEI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14765
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2795_length_14765_cov_5.037322"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1634
                     /locus_tag="P5313_09075"
     CDS             33..1634
                     /locus_tag="P5313_09075"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY80556.1"
                     /translation="MSNESPLLSFESITKDFYGTVVLKDVSFTLGRGRILGLVGENGA
                     GKTTLMSVLFGMPVISETGGFSGTVKLDGQAVSFRTPFDALDAGIGMVHQEFSLIPGF
                     TTTENIVLNRESVKPNPAVEVFGERMSVLDRPKMRARAESAIGRLGVKLDPDMLVSEM
                     PVGHKQFTEIAREINRDNVRLLVLDEPTAVLTESEAEVLLAALRNLAAQGISIIFISH
                     RLHEVLSVCDRIVTLRDGRVVKDTDAAGVTVADVASWMVGRDVDDAGRKGAARDLPGA
                     ALEVENLWVDMPGETVRNVSFSVREGEIFGIGGLAGQGKLGIPNGIMGLYPAGGTVRL
                     HGQAVTLDAPRAALDAGMAFVSEDRRGVGLLLDESLDWNIAFTAMQIKGEYLKTYLGG
                     LVRLRDEKAMKALTEEYVKLLDIKCTGPKQKAKELSGGNQQKICLAKAFALRPSVLFV
                     SEPTRGIDVGAKALVLDNLRHYNRDFGTTIVMASSELEELRSICDRVAIVDEGRIAGI
                     LPPGAPLSEFGLLMAGQKVSEEVQP"
     gene            1631..2674
                     /locus_tag="P5313_09080"
     CDS             1631..2674
                     /locus_tag="P5313_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY80557.1"
                     /translation="MNRLKQAVTDFGWPRIIIFLFVVALFIGAPFVKVRVDSSFTDVF
                     NRFGQNAVLVLALVPMVQAGCGLNFGLPVGIIAGLLGSTLAMEFGLVGWAGFLGAIGL
                     SLPFAVAFGFAYGLLLNKVKGEEMTIAMYVGFSIVTFFAILWLLLPYSSPNMVWGYAG
                     KGLRTTITLEGYWGLILNDFLALRIGENFRFPTGMILFVILLTVLVGFFFRSKTGTAM
                     TAVGSNPDFARAGGVSADRMRLTSVILSTVIGAVGILVYQQSFGFIQIYNAPLFMAFP
                     AVAALLIGGASVNKASMLNVIVGTFLFQSILTMTPSVINSILQTDMSEVIRILISNGM
                     ILYALTRKTKVTK"
     gene            2671..3783
                     /locus_tag="P5313_09085"
     CDS             2671..3783
                     /locus_tag="P5313_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY80558.1"
                     /translation="MSKTFDPRKFLADNLVVVIFLVVTAFAVPVSGLSAQFIIQELFT
                     RIGRNAFLVFSLLLPIMAGMGINFGMVLGAMAGEIGLIIAVDWGILGVNGLAFASLVG
                     MPIAVFLGWIAGEVLNRAKGREMVTGYILGFFMDGVYQLVVLYMMGYIIPIRSPAITL
                     SRGYGIRNTLGLDSVRQSLDNLLAVRIGPYVLPLATYMVIGLLCVFIIWFRRTKLGQD
                     MRAIGQDQGVSHSAGISVERTRIIAIMISTVLAMIGQIIFLQNMGNMATYNAHKQTGF
                     FAAAALLVGGASVSKATIPNVFIGVVLLHLMYIVMPRAGAQLFGEAQIGEFFRDALSY
                     AAIAMALVIYAWRKRANAERARAGLRGGEAAAGGVK"
     gene            3780..4253
                     /locus_tag="P5313_09090"
     CDS             3780..4253
                     /locus_tag="P5313_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80559.1"
                     /translation="MSKLDPKAARRWAIRAVLAAVYLALAVILFVTGKGHTILVDNKA
                     DPAGAYPALKAAVVSIGRQKPMEFYPGDRDKFVVKGQRHRIRVKPLAPGAQEREVEFR
                     LPLGQDMTLLSISKLMAGAEPWIEVFVAPTVQQMAAERAADDLANPLETFGNTGP"
     gene            4276..4767
                     /locus_tag="P5313_09095"
     CDS             4276..4767
                     /locus_tag="P5313_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80560.1"
                     /translation="MKRCLAAALAAFVLAGAGFAQTPPKVLSPYDIERFIKDIPGLMA
                     DLEAAGSDFDEEVAQSQDDPASFSPASLKSLLAKARTSAEVRRTLEKYNWDGRFWDVY
                     YTIYVGVYVSMMDQALVQYPSPELKALVDPFRTSIHKDDHALVLRNLPRLLEVFEALD
                     AED"
     gene            5140..7602
                     /gene="leuS"
                     /locus_tag="P5313_09100"
     CDS             5140..7602
                     /gene="leuS"
                     /locus_tag="P5313_09100"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRY80561.1"
                     /translation="MAKYPYTEIESKWQRYWDEHKTFKAVEDPGIPADKRVYVLDMFP
                     YPSGAGLHVGHPEGYTATDIYCRFLRMNGYAVLHPMGFDSFGLPAENYAIQTGTHPAA
                     TTSANIDRFRTQIKSLGFSYDWDREVSTCDPDYYKWTQWIFLKLFERGLAYEAEAPIN
                     WCPKCKTGLANEEVKDGRCDRCETPVVRKSIRQWILRITAYADRLLADLDTLDWSESI
                     KLMQRNWIGRSEGANVVFKMAEGEGEIEVYTTRPDTLFGATYMVLAPEHALVSKITTP
                     DRKAAVEAYVAEAARKSDLERTDLAKDKTGEFTGAYAVNPVNGRRIPVWISDYILASY
                     GTGAIMAVPAHDERDWDFAKKFGLPIVQVVAAPGSEEARSGEVRPEEGPAEPSECTSA
                     EGVAINSGKFDGLPTAEAIKAITEWLAEKGIGRKAVNYKLRDWIFSRQRYWGEPIPVV
                     HCKECGIVPLPESELPLRLPDVKTYAPTGTGESPLANIHDWVNTTCPKCGGPAKRETN
                     TMPQWAGSCWYYLRFIDPKNDKAFAAKDKIDYWMPVDLYVGGAEHAVLHLLYSRFWHK
                     VLYDIGAVNTIEPFQRLVNQGMILGEDNQKMSKSRGNVVNPDDIVRDFGADAMRVYEM
                     FMGPLEVSKPWATAGLVGVSRFLERLWALSEKPLTGAAPSPDLAKLLHKTIKKVTDDT
                     ATLNFNTAISAMMILSNELARLDVLPREVWEAFVRLLAPYAAHLAEELWEKAGKAPSV
                     GLAPWPSYDETLVRDDTAVIVVQVNGKVRDKFEAPAGTDKAELERLALGREKTREWIA
                     GKPLRKVIIVPDKLVNIVVGEG"
     gene            7633..9447
                     /locus_tag="P5313_09105"
     CDS             7633..9447
                     /locus_tag="P5313_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HRY80562.1"
                     /translation="MARRALSNIGILGKLTVLGAVFLLSLGGVAYVSLKAFRSLTATM
                     DNILEVQNKKVRRFEDLENQAYAVQVNLYRAINYTLSGGDAREDAIRAFNTALAGFKT
                     SVDTFTEETLDTLEYDESGNPKGLTGADFDSYFNTARVVQSYLREDARRALDVMVQVD
                     STFEELKERIWVEGSAARTAASEVESRARKSAAAASLTVISVSGAVVLASLLLILLTV
                     RSITLPLRKLEDTLAQAGRGDLRTCSDMDGRDEMGRMGRSVDALVCGIRDLVSTVMEK
                     VRDLESTGQSLAANMEETGAAVLQINAGIGNTRGQLDQQTESVQEVSAAVEQLARSVT
                     SLSDRIETQSDVIGQSSAATEEIIASVESVARAADSAARASEELTAEGVQGKERIDEA
                     GDSVRDIARHSENLTEAVRLISEIASRTNLLAMNAAIEAAHAGDAGRGFAVVADEIRR
                     LAEQSTAQAKDIAGGLGQVSRSIEKVQDASLSAVESFGTVLAKADALAAEIRRINASM
                     AEQREGGRLVLEDLSRLKDLTREISYGAEEMKSGNRTILEQVERLTSVNQLVIQNNAE
                     ITTGTKEINDAIAATMDLASRTASSIGEVKAALDTFEI"
     gene            complement(9534..10157)
                     /locus_tag="P5313_09110"
     CDS             complement(9534..10157)
                     /locus_tag="P5313_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80563.1"
                     /translation="MDRLARAERARQIFDPHSLAAAGLVVVAALLFQPRPGPRAAILL
                     GAMVFTWATGRKVPVLGTGLVILGIVGANLLVPVGRVLAVWGPIRVTETALLEGVEKA
                     VTFEALIYVSKAAIRSDLRIPGRAGALIGSALRCYERILETRIRIRRATFLRDLDEIL
                     LGIYDEELSKTPGRPAVVRTGRGGAGTVFLVLAAAAAWLPYLVRAIR"
     gene            complement(10150..10698)
                     /locus_tag="P5313_09115"
     CDS             complement(10150..10698)
                     /locus_tag="P5313_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HRY80564.1"
                     /translation="MTDRTSIPAPGDSRARLLAFLSALSFFLSAVEYMIPKPLPFMRL
                     GIANLPLLLAVDLLPVGPYLALAAIKVLGMSLVTGSLFSYVALFSLAGTFCAALVMFG
                     ARTLFRTRMTAVGISVLGAVSSNAVQVVLASLVVFGRAARIIAPVFLAMGLATGLALG
                     IFAERFAASSRWYARAREDARG"
     gene            complement(10664..11119)
                     /locus_tag="P5313_09120"
     CDS             complement(10664..11119)
                     /locus_tag="P5313_09120"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HRY80565.1"
                     /translation="MGAAAENGGTRGSGPEAPTEPPAAKPGKLRLTFLDAAAVLAAAS
                     LVFLSSLGAVDAAGEPRVVIADAKGQEWIYPLDTDLEVPIEGPLGISTVHIHDGRVAI
                     ESSPCPNQTCVAAGSIDRPGQWVACLPNQVFVRVEGGTDDGSDVDTGTW"
     gene            complement(11103..11633)
                     /locus_tag="P5313_09125"
     CDS             complement(11103..11633)
                     /locus_tag="P5313_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY80566.1"
                     /translation="MVVEKDKVITIDYTLKDSDGQTIDSSDDSEPLVYLHGNDNLIPG
                     LEKQLEGKKEGDRLSCVINPGEAYGDYDESLVFTVQKSNFADPSKIEEGMQFEAHGED
                     GPRVVTVVAVKGDEVTVDANHPLAGEDLHFDVKVVGIREATAEELAHGHVHSHEGCGD
                     DCDCEDEGGCGCGCCG"
     assembly_gap    11909..12008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12139..12942
                     /locus_tag="P5313_09130"
     CDS             12139..12942
                     /locus_tag="P5313_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRY80567.1"
                     /translation="MTFMQALILGLFQGVSEFLPVSSSGHLLALRELFGLSEVPRLFD
                     VILHAATLLSILVVFRARIGGILAALVRWAARRGDVPAQDAENLALVPPALAATAVTA
                     VTGLGIEKFLPVESPRIASAFLLVTAALLIGSSRFRGTRGYGRLGLGRGALIGLAQGI
                     GVLPGISRSGITISAGLAAGLDRDTAGEFAFLLAVPAVAGALLLELKDLADLGAVVAP
                     APLAVGFLVAFASGIASLSLLMPLVRKGRLAWFAAYLIPAGLAGLFLFR"
     gene            13023..>14765
                     /locus_tag="P5313_09135"
     CDS             13023..>14765
                     /locus_tag="P5313_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRY80568.1"
                     /translation="MNNDGSRKSGLPGRVGIVLAVLGALSAVIVAAGLAAGPGAGFPA
                     SAGRLLTRPLGWAALCLPVFLFAASGVLFLPGYRKDLLVLLSGAPLPFLVLVFLARFL
                     AEPGPWYDAYPLLEALGLPGTTALGVLVLLLSITLLVRLKGLAGRGDSGTPEAEEDEE
                     GAAAPTAPKDAAPRAPAARPAPSVPDGSGSGRSKFVPLVPEPGEPPFPSPGLVPPAAL
                     ERGSPGGSEDALSEFEEEAALEELEGPDGESGGDRETEPPIPVLFRRTEPEPGPKPET
                     ASSARPPLLAGVAADPTAEAEMRPSRGSPFGSGAPGAGADADGAAADGSPVGRHPLPV
                     FESVPPSPRRAPKTIHIAAYSMPTDILTVYPDGQYWVIDDKTRRSADVLRDTLAEFNI
                     EAEVTGIRRGPVVTMFEILPAPGVKLSKIVNLSDNIALRLAASSVRIVAPIPGKHAVG
                     IEVPNDKRSVVSFREIVETDVFRAAKFEIPLALGKDIAGEVQIIDLTQMPHLLIAGAT
                     GSGKSVLVNSIILSILYRKSWNEVKLLLIDPKIVELKLYNGIPHLLTPVVTEPKRAFQ
                     ALQYCICEMERRYSL"
BASE COUNT         2368 a         5003 c         4895 g         2399 t
ORIGIN      
        1 cccccggccg attccgcacg gaaggtgtcc ctatgtcgaa tgaatcgccc ttattgagtt
       61 tcgaaagcat caccaaggac ttctacggga ccgtggtcct caaagacgtg tccttcaccc
      121 tgggcagggg caggatcctg ggcctggtcg gggagaacgg cgcgggcaag accaccctga
      181 tgagcgtcct gttcgggatg cccgtcatct cggagaccgg agggttttcc gggaccgtga
      241 agctggacgg ccaggccgtc tccttccgaa cccccttcga cgccctggac gcgggcatcg
      301 ggatggttca ccaggagttc tccctgatac ccggcttcac gaccacggag aacatcgtcc
      361 tgaaccggga atccgtaaag cccaatccgg ccgtggaggt cttcggagaa cggatgtccg
      421 tcctggaccg tccgaagatg cgggcccgtg cggagtccgc gatcggccgc ctgggtgtca
      481 agctggaccc ggacatgctc gtttccgaga tgcccgtggg acacaagcag ttcacggaga
      541 tagcccggga gatcaaccgg gacaacgtcc ggctcctggt gctggacgag cccaccgcgg
      601 tcctgacgga gagcgaggcg gaggtcctat tggccgccct ccggaacctg gccgcccagg
      661 gcatctccat catcttcata tcccaccgcc tccacgaggt cctgtccgtc tgcgaccgga
      721 tcgtgaccct ccgggacggc cgggtggtca aggacaccga cgcggcgggg gtgaccgtag
      781 cggacgtggc ctcctggatg gtgggccggg acgtggacga cgccggccgc aagggtgccg
      841 cccgggacct tcccggcgcg gccctggagg tggagaacct ctgggtggac atgcccgggg
      901 agaccgtccg gaacgtctcc ttctccgtgc gggaggggga gatcttcggc atcggggggc
      961 tcgccggcca gggaaagctg ggcatcccga acgggatcat gggcctgtac cccgcgggtg
     1021 ggacggtccg gcttcacggc caggccgtga ccctggacgc cccccgggcg gccctggacg
     1081 cgggcatggc cttcgtcagc gaggaccgcc ggggcgtggg gctcctcctg gacgagagcc
     1141 tggactggaa catcgccttc acggccatgc agatcaaggg cgagtacctg aagacctacc
     1201 tggggggcct ggtgcggctc cgggacgaga aggcgatgaa ggccctcacg gaggagtacg
     1261 tcaagctcct ggacatcaag tgcacgggtc ccaagcagaa ggccaaggag ctgtccgggg
     1321 gcaaccagca gaagatctgc ctggccaagg ccttcgccct caggccctcg gtcctcttcg
     1381 tctccgaacc cacccggggc atcgacgtgg gcgccaaggc cctggtgctg gacaacctgc
     1441 gccactacaa ccgcgacttc ggcaccacta tcgtgatggc ctccagcgag ctcgaggagc
     1501 tccggtccat ctgcgaccgc gtggccatcg tggacgaggg ccggatcgcg ggcatcctgc
     1561 cccccggcgc cccgctctcc gagttcgggc tcttgatggc gggtcagaag gtttccgagg
     1621 aggtccagcc gtgaacagac ttaagcaggc cgtcaccgat ttcggctggc cccgcatcat
     1681 catcttcctc ttcgtggtgg ccctgttcat cggggcgccc ttcgtcaagg tccgggtgga
     1741 ttcgtccttc acggacgtct tcaaccgctt cgggcagaac gcggtcctgg tcctggccct
     1801 ggtgcccatg gtccaggcgg gctgcgggct caacttcggc ctgcccgtcg ggatcatcgc
     1861 gggcctcttg ggctccacgt tggccatgga attcggcctt gtcggttggg cgggcttcct
     1921 gggggccatc ggcctctccc ttcccttcgc ggtcgccttc ggcttcgcct acgggctgct
     1981 gctgaacaag gtcaagggcg aggagatgac catcgcgatg tacgtgggct tctccatcgt
     2041 caccttcttc gcgatcctgt ggctcctcct gccgtattcc agcccgaaca tggtctgggg
     2101 ctacgcgggc aagggactgc ggaccaccat caccctggag gggtactggg gactcatact
     2161 caacgacttc ctggccctcc ggatcgggga gaacttccgg ttccccacgg gcatgatcct
     2221 cttcgtgatc ctgctgacgg tcctggtggg gttcttcttc cgcagcaaga ccggcacggc
     2281 catgaccgcc gtgggctcca acccggactt cgcccgggcg ggcggggtgt ccgcggaccg
     2341 gatgcgcctg accagcgtga tcctgtccac cgtgatcggc gcggtcggca tcctggtcta
     2401 ccagcagagc ttcgggttca tccagatcta caacgccccc ctgttcatgg ccttcccggc
     2461 ggtggcggcc ctgctgatcg gcggggcctc ggtgaacaag gccagcatgc tgaacgtcat
     2521 cgtgggcacc ttcctcttcc agtccatcct gaccatgacc ccgtcggtca tcaacagcat
     2581 cctgcagacg gacatgtccg aggtgatccg catcctgatc tcgaacggca tgatcctgta
     2641 cgccctgacc cgaaagacga aggtgacgaa atgagcaaga cgttcgaccc gcgcaagttc
     2701 ctggcggaca acctggtggt cgtcatcttc ctggtggtga cggccttcgc ggtccccgtg
     2761 tccgggctct ccgcgcagtt catcatccag gagctgttca cccgcatcgg ccgcaacgcg
     2821 ttcctggtct tctccctcct cctgcctatc atggcgggca tgggcatcaa cttcggaatg
     2881 gtcctggggg ccatggccgg ggagatcggc ctcatcatcg ccgtggactg gggcatcctg
     2941 ggggtgaacg gcttggcctt cgcctccctg gtgggcatgc ccatcgccgt cttcctgggc
     3001 tggatcgccg gggaggtcct gaaccgcgca aagggccggg agatggtcac cggctacatc
     3061 ctgggcttct tcatggacgg ggtgtaccag ctggtcgtcc tttacatgat ggggtacatc
     3121 atccccatcc gctccccggc catcaccctg tcccggggct acggcatccg gaacaccctg
     3181 ggcctcgact cggtccgcca gtccctggac aacctcctgg cggtccggat cggaccctac
     3241 gtcctgcccc tggccaccta catggtcata gggctcctgt gcgtcttcat catctggttc
     3301 cgcaggacca agctgggcca ggacatgcgt gcgatcgggc aggaccaggg ggtttcccac
     3361 tccgcgggga tctccgtgga gcggacccgg atcatcgcca tcatgatctc aacggtcctg
     3421 gccatgatcg gccagatcat cttcctccag aacatgggaa acatggccac ctacaacgcc
     3481 cacaagcaga ccgggttctt cgcggccgcg gccctcctgg tgggaggcgc ctcggtctcc
     3541 aaggccacca tcccgaacgt cttcatcggt gtggtgctcc tgcacctgat gtacatcgtg
     3601 atgccccggg cgggcgccca gctcttcggg gaagcccaga tcggggagtt cttccgggac
     3661 gcactgtcct acgcggccat cgcgatggcc ctggtcatct acgcctggcg gaagcgggcc
     3721 aacgcggagc gggcccgggc cgggctccgg ggcggggaag ccgccgcggg aggcgtgaaa
     3781 tgagcaagct cgatcccaag gccgcccgca ggtgggccat ccgggccgtc cttgcggccg
     3841 tctacctggc gctggcggtc atcctcttcg tgaccgggaa gggacatacg atcctggtgg
     3901 acaacaaggc agatcccgcg ggagcctatc ccgccctgaa ggccgccgtg gtctccatcg
     3961 gccgtcagaa gcccatggag ttctaccccg gggaccggga caagttcgtc gtgaagggtc
     4021 agcggcaccg gatcagggtg aagcccctcg ccccgggagc ccaggagcgg gaggtcgagt
     4081 tccggctgcc cctgggccag gacatgaccc tgctgtctat ctccaagctg atggccgggg
     4141 cggagccctg gatcgaggtc ttcgtggctc cgacggtaca gcagatggcc gcggagcgcg
     4201 cggcggacga cctggccaac cccctggaga ctttcgggaa caccggacct tgagggcggc
     4261 cgcgcgggag gagccatgaa acgatgcctt gcggccgccc tggcggcctt cgtgctggcc
     4321 ggagcggggt ttgcccagac cccgcccaag gtgctctcgc cctatgacat cgagcgcttc
     4381 atcaaggaca tccccggcct gatggcggac ctggaggccg cgggatcgga cttcgacgag
     4441 gaggtggccc agtcccagga cgatccggcc tccttcagcc cggcctccct caagtccctc
     4501 ctggccaagg cccggacctc cgcggaggtc cggcggaccc tggagaagta caactgggac
     4561 ggccggttct gggacgtcta ctacacgatc tacgtcgggg tctacgtctc catgatggac
     4621 caggccctgg tccagtaccc cagccccgag ctcaaggccc tggttgatcc cttccggacc
     4681 tcgatacaca aggacgacca cgccctggtc ctgcggaacc tcccgcgcct gctggaggtc
     4741 ttcgaggccc tggacgcgga ggactgagcc ggccgttccc aggtgtttcc ggaatcaacc
     4801 acggagatca cggagggcac ggagaaatat aaaagaatag gcgactgaga caggataaaa
     4861 ggatcctgca ggattgacag gatagagagc accattgttt cagttctggc atgaatagat
     4921 ctcatcctgt tcatcatgga aatcctgtta tcctgtctcc tttttttact tcatctctcc
     4981 tccttcttct ccgtgaactc cgtgtcctcc gtggtgagtt cctgaaattt taaaaccggg
     5041 tattttttaa cctctgccgc cctgcccttg tccggccccc cgggcttcct gtatcatcct
     5101 ccggaacagc ttgatcttcc ccggatccag gagtgaccca tggccaagta cccgtacacc
     5161 gagatcgagt ccaagtggca gcgctactgg gacgagcaca agaccttcaa ggccgtcgag
     5221 gacccgggca tccccgcgga caagcgggtc tacgtcctgg acatgttccc ctatccctcc
     5281 ggggcgggcc tccacgtggg gcatcccgag ggctacaccg ccacggacat ctactgccgc
     5341 ttcctgcgga tgaacgggta cgcggtcctc catcccatgg gcttcgactc cttcggcctg
     5401 cccgcggaga actacgccat ccagacgggc acccaccccg cggccaccac ctcggccaac
     5461 atcgaccggt tccggaccca gatcaagtcc ctgggcttct cctacgactg ggaccgggag
     5521 gtctccacct gcgatcccga ttactacaag tggacccagt ggatcttcct caagctcttc
     5581 gagcggggcc tggcctacga ggccgaggct ccgatcaact ggtgccccaa gtgcaagacg
     5641 ggcctggcca acgaggaggt caaggacggg cgctgcgacc gctgcgagac ccccgtggtg
     5701 cgcaagagca tccgccagtg gatcctccgg atcaccgcct acgcggaccg gctcctggcc
     5761 gacctggaca ccctggactg gtcggagtcc atcaagctga tgcagcgcaa ctggatcggc
     5821 cggagcgagg gcgccaacgt ggtcttcaag atggccgagg gcgaggggga gatagaggtc
     5881 tatacgaccc ggccggacac cctcttcggg gccacctaca tggtcctggc cccggagcac
     5941 gccctggtgt cgaagatcac caccccggac cggaaggccg ctgtggaggc ctacgtggcc
     6001 gaggcggctc ggaagagcga cctggagcgc acggacctgg ccaaggacaa gaccggcgag
     6061 ttcacggggg cctacgcggt caaccccgtc aacggacgca ggatccccgt gtggatctcg
     6121 gactacatcc tggcctccta cgggaccggg gccatcatgg ccgtgccggc ccacgacgag
     6181 cgggactggg acttcgccaa gaagttcggc ctgcccatcg tgcaggtggt ggcggccccg
     6241 ggcagcgagg aggcccggag cggggaggtc cggcccgagg agggccccgc tgaaccgtcg
     6301 gagtgcacct ccgcggaggg cgtcgcgatc aactccggga aattcgacgg cctgcccacg
     6361 gccgaggcca tcaaggccat caccgaatgg ctggcggaga aggggatcgg ccgcaaggcg
     6421 gtgaactaca agctccggga ctggatcttc tcccgccagc gctactgggg cgagcccatc
     6481 cccgtggtcc actgcaagga atgcggcatc gtgcccctgc ccgagagcga gctgcccctg
     6541 cgcctgccgg acgtcaagac ctacgccccc acgggcaccg gggagtcccc cctggcgaac
     6601 atccacgact gggtgaacac cacctgcccg aagtgcggcg gaccggccaa gcgggagacg
     6661 aacaccatgc cccagtgggc gggctcctgc tggtactacc tgcgcttcat cgacccgaag
     6721 aacgacaagg ccttcgcggc caaggacaag atcgactatt ggatgcccgt ggacctctac
     6781 gtgggcgggg cggagcacgc ggtgctccac ctcctctact cccggttctg gcacaaggtc
     6841 ctctacgaca tcggggcggt gaacaccatc gagcccttcc aacgcctggt gaaccagggc
     6901 atgatcctgg gcgaggacaa ccagaagatg tccaagagcc ggggcaacgt ggtgaacccc
     6961 gacgacatcg tccgggactt cggcgccgac gccatgcggg tctacgagat gttcatgggg
     7021 cccctggagg tctccaaacc ctgggccacc gcgggactcg tgggggtcag ccgcttcctg
     7081 gaacgcctgt gggccctctc ggagaaaccc ctgaccgggg cggctccgtc cccggacctc
     7141 gccaagctcc tgcacaagac catcaagaag gtgacggacg acaccgccac cctgaacttc
     7201 aacaccgcca tcagcgccat gatgatcctg tccaacgagc tggctcggct ggacgtcctt
     7261 ccccgggaag tctgggaagc cttcgtccgg ctcctggcgc cctacgccgc acatctggcg
     7321 gaggagctct gggagaaggc gggcaaggcc cccagcgtcg ggctggcccc ctggccgtcc
     7381 tatgacgaga ccttggtgcg ggacgacacg gcggtcatcg tggtccaggt gaacgggaag
     7441 gtccgggaca agttcgaggc tcccgcgggt acggacaagg cggagctcga gaggctcgcc
     7501 ctggggcggg agaagacccg ggagtggatc gcggggaaac cgctacgcaa agtgatcatc
     7561 gtccccgata aactagtgaa cattgtggtc ggggagggct gagccgcccg ttcccgaact
     7621 gccggaggtg tcatggcacg gcgtgcgctg tcgaatatcg ggatcctggg gaagttgacg
     7681 gtcctggggg ccgtcttcct gctctccctg ggaggggtgg cctacgtgtc cctgaaggcg
     7741 ttccggtccc tgaccgccac gatggacaac atcctggaag tccagaacaa gaaggtccgt
     7801 aggttcgagg acctggagaa ccaggcctac gcggtgcagg tcaacctgta ccgggcgatc
     7861 aactacaccc tgtccggggg ggacgcccgg gaggacgcca tccgggcgtt caacacggcc
     7921 ttggcgggct tcaagacctc cgtggatacc ttcacggagg agaccctgga taccctcgag
     7981 tacgacgagt ccggaaaccc caagggccta acgggggcgg atttcgattc ctacttcaac
     8041 accgcccggg tcgtccagag ctacctccgg gaggacgccc gccgcgccct ggatgtcatg
     8101 gtccaggtcg attcgacctt cgaggagctc aaggagcgga tctgggtgga gggctccgcc
     8161 gcgcggaccg cggcctccga ggtcgagtcc cgggcgcgca agtccgcggc cgcggccagc
     8221 ctgaccgtga tctcggtgtc cggcgcggtc gtcctggcct ccctcctcct gatcctgctg
     8281 acggtccggt ccataaccct gcccctgcgg aagctggagg ataccctggc ccaggccggc
     8341 cggggggatc tccggacctg ctcggacatg gacggacggg acgagatggg ccgcatgggc
     8401 cggagcgtgg acgccctggt ctgcgggatc cgggacctcg tgtccacggt catggagaag
     8461 gtccgggacc tggagtccac gggccagagc ctggcggcga acatggagga gaccggggcc
     8521 gcggtcctcc agatcaacgc gggcatcggg aacacccggg gccagctgga ccagcagacc
     8581 gaatccgtcc aggaggtctc cgcggcggtg gagcagctgg cccggagcgt cacctccctg
     8641 tcggaccgga tcgagaccca gtcggacgtg atcgggcaat cctcggcggc cacggaggag
     8701 atcatcgctt ccgtggagtc cgtagcccgc gccgcggact cggcggcccg ggccagcgag
     8761 gagctgacgg ccgagggggt ccagggcaag gagcggatcg acgaggcggg cgattccgtg
     8821 cgggacatcg cccggcactc ggagaacctc acggaggcgg tccgccttat ctccgagata
     8881 gccagccgca cgaacctgct ggccatgaac gccgctatcg aggccgccca cgccggggac
     8941 gcgggccggg gctttgcggt ggtggcggac gagatccgaa ggctcgcgga gcagtccacg
     9001 gcccaggcca aggacatcgc gggggggctc gggcaggtgt cccggtcgat cgagaaggtc
     9061 caggacgcgt ccctgtccgc ggtggagtcc ttcggcaccg tgctggccaa ggcggacgcc
     9121 ctggccgcgg agatccggcg catcaacgcc tccatggccg agcagaggga ggggggccgc
     9181 ctggtcctgg aggacttgtc ccggctcaag gacctcaccc gggagatctc ctacggggcg
     9241 gaggagatga agtccggcaa ccggaccatc ctggaacagg tggagcgcct gacctcggtg
     9301 aaccagctcg tgatccagaa caacgcggag atcacgacgg gcaccaagga aatcaacgac
     9361 gccatcgccg cgaccatgga cctggcctcc cggaccgcct cctcgatcgg ggaggtcaag
     9421 gcggccctgg ataccttcga aatctaacgg gttgtcgaaa tgcctcgagc atttcgacaa
     9481 cccgttttgc agtttctcga gaaactgcgg cattagtgta gtgtcacact gctttaacgg
     9541 atcgcccgga ccaggtaggg aagccaggcg gccgcggccg ccaggaccag gaagaccgtc
     9601 cccgcccctc cccgcccggt ccgcacgacg gccggacggc ccggcgtctt cgagagttcc
     9661 tcgtcgtaga ttcccagcag gatctcgtcc aggtcccgga ggaaggtggc gcgccggatc
     9721 cggatccggg tttccaggat cctctcgtag cagcgcaggg cggagccgat cagggccccc
     9781 gcgcggcccg ggatccgcag gtccgatcgg atggcggcct tggaaacgta gatcagggcc
     9841 tcgaaggtga cggccttctc cacgccctcc agcagggcgg tctcggtcac ccggatcggc
     9901 ccccagaccg ccagcacccg gccgaccgga accagaaggt tagcgcccac gatacccagg
     9961 atgaccaggc ccgtgcccag gacgggaacc ttccgccccg tcgcccaggt gaacaccatg
    10021 gcccccagaa ggatcgccgc ccggggaccc gggcggggtt ggaagaggag ggcggcgacg
    10081 acgaccagtc ccgcagccgc cagggaatgg gggtcgaaga tctgccgggc tcgctccgcg
    10141 cgggcgagcc tatccacggg cgtcctcccg ggcccgggcg taccaccggg aggaggccgc
    10201 gaaccgctcg gcgaagatcc ccagggccag gccggtggcc aggcccatgg ccaggaagac
    10261 cggggcgatg atccgggccg cccggccgaa gacgaccagg gaggccagga ccacctggac
    10321 ggcgttggag gagacggcgc ccaggacgct gattcccacc gcggtcatcc gggtccggaa
    10381 gagggtccgg gctccgaaca tcaccagggc ggcgcagaag gttcccgcca gggagaacag
    10441 ggccacgtac gagaagaggg agcccgtcac caggctcatc cccaggacct tgatcgccgc
    10501 cagggccagg tagggaccga cgggcaggag gtccacggcc aggaggaggg gcaggttggc
    10561 gatccccagg cgcatgaagg gcaggggctt ggggatcatg tactccaccg cggagaggaa
    10621 gaaggagagg gcggacagga aggccaggag gcgggcccgg gaatcaccag gtgccggtat
    10681 cgacgtccga tccgtcatcg gtccctcctt ccacgcggac gaagacctgg ttcggcaggc
    10741 aggcgaccca ctggcccggg cggtcgatgg atcccgcggc cacgcaggtc tggttggggc
    10801 agggggagct ctcgatggcg acccggccgt cgtggatgtg gaccgtcgag atccccaggg
    10861 ggccctcgat cgggacctcc aggtccgtat ccagggggta gatccactcc tgtcccttcg
    10921 cgtcggcgat cacgacccgg ggttctcccg cggcgtcgac ggcgcctagg gagctcagga
    10981 aaacgaggga cgcggccgcc agtacggccg ccgcgtccag gaacgtgagc cggagcttcc
    11041 cgggcttggc cgcgggcggc tccgtcggag cctccgggcc ggatccccgg gtcccgccgt
    11101 tctcagccgc agcacccaca gccgcagccg ccctcgtctt cgcagtcgca gtcgtcaccg
    11161 caaccctcgt gggagtgcac gtggccgtga gccagttcct cggcggtggc ctcccggatc
    11221 cccacgacct tgacgtcgaa gtggaggtcc tccccggcca gggggtggtt ggcgtccacg
    11281 gtcacctcgt cgcccttgac ggccaccacg gtcacgaccc gggggccgtc ctcgccgtgg
    11341 gcctcgaact gcatcccctc ctcgatcttg gagggatccg cgaagttgga cttctggacc
    11401 gtgaacacga ggctctcgtc gtagtccccg taggcttctc cggggttgat gacgcaggac
    11461 agcctgtcgc cctccttctt gccctccaac tgcttctcca ggcccgggat caggttgtcg
    11521 ttgccgtgga gatagacgag gggttccgaa tcgtcggaac tgtcgatcgt ctggccgtcg
    11581 gaatccttca gggtataatc gatcgtgata accttgtctt tttctacgac catggtaacc
    11641 gccttttcgt ggaaatatgg cagacaggct cgcttcgggc cgggcgtgat ggcgcaggca
    11701 gtgtcggaag gtccgatgcc gggtaggagc gtccggaatg cccgggtact gtatactaac
    11761 ggcgcgtaac cggcaagtgg cgggaacgga ggtccgcggg gcggacctcc gttcccggcg
    11821 acgagctgac gctcgtcgag gcttctcggg gccggtccgc ggggcggacc tccgttcccg
    11881 gcgacgagct gacgctcgtc gaggcttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnngg ccggtccgcg gggcggacct ccgttcccgg cgacgagctg acgctcgtcg
    12061 aggttgtttc agggatcgat ccgcggggcg gaccggttcc ccttcccacg ctcggggagc
    12121 cccgctatag tggctcccat gacattcatg caagccctga tcctgggcct tttccaggga
    12181 gtatccgagt ttcttcccgt atcctccagc ggccacctcc tggccctccg ggagctcttc
    12241 ggcctttccg aggtgccccg gcttttcgac gtgatcctgc acgcggcgac cctcctgtcc
    12301 atcctggtag tcttccgagc gcggatcggc ggcatcctgg cggccctggt ccgatgggcc
    12361 gcccgacgcg gggacgtccc ggcccaggac gcggaaaacc ttgccctggt gccgcctgcc
    12421 ctggccgcca cggccgtcac ggcggtcacg ggtctgggga tcgagaagtt cctgccggtg
    12481 gagtctcccc gcatcgcctc ggcgttcctc ctggtcacgg cggcgctgct gatcggttcc
    12541 tcccggttcc ggggaacccg gggttacggc cgtctcggcc tcggccgggg cgccctgatc
    12601 ggcttggccc aggggatcgg cgtcctgccc ggcatcagcc ggtccggtat caccatttcc
    12661 gcggggctgg cggcgggcct ggatcgggac acggccgggg agttcgcctt cctcctggcc
    12721 gttccggcgg tggccggggc cctcctcctg gagctgaagg acctggccga cctgggcgcc
    12781 gtcgtcgcgc ccgctcctct ggccgtcgga ttcctggtcg ccttcgcctc cgggatcgcc
    12841 tcgctttccc tgctcatgcc cctggtgcgg aagggccgtc tggcctggtt cgcggcctat
    12901 ctgatccccg cgggtcttgc cggtctcttc ctgttccgct gatccgccga taatctactg
    12961 atgctgtccc cgacggcctg tcgggccggg ggacgcgtac aaccacaggt ggagtccagg
    13021 gtttgaacaa cgacggatcg aggaagtcgg gtctgccggg gcgcgtcggg atcgtcctgg
    13081 cggtcctggg cgcattgtcc gctgtcatcg tggccgcggg actggccgcg gggcccgggg
    13141 cgggttttcc ggcctccgcg gggaggctcc tgacccgacc cctgggctgg gcggccctct
    13201 gtctcccggt cttcctcttc gcggcctccg gggtgctctt ccttccgggg taccgaaagg
    13261 acctcctggt tctcctctcc ggggcgcccc ttcccttcct ggtcctggtc ttcctggccc
    13321 ggttcctggc cgagcccggc ccctggtacg acgcctaccc cctcctggag gccctgggcc
    13381 tgccggggac caccgcgctg ggcgtcctgg tcctcctgct ctcgataacc ctcctggtcc
    13441 ggctcaaggg actcgcgggc cggggggatt ccgggactcc cgaggcggag gaggacgagg
    13501 agggagcggc cgccccgacg gccccgaagg atgccgcccc ccgggctccc gccgcccgtc
    13561 cggctccctc cgtcccagac ggatcgggat ccggccgctc caaattcgtc cccctcgtgc
    13621 ccgaacccgg cgaacctccc ttcccgtccc ccggtctggt cccgccggcc gccctggaac
    13681 gcgggtcgcc cgggggctcc gaggacgcct tgtcggagtt cgaggaggaa gccgccctgg
    13741 aggagcttga gggtccggac ggggaatccg gcggggatcg ggagaccgag ccccccatcc
    13801 ccgtcctgtt ccgccgcacc gagcccgagc cggggccgaa gccggagacc gcatcctcgg
    13861 cccggccgcc cctcctggcc ggggtggccg cggaccccac ggcggaggcg gagatgcgcc
    13921 ccagccgggg ctcgcccttc ggatcgggag ccccgggggc cggcgcggac gcggacggcg
    13981 cggcagccga cggatccccc gtgggccgtc atcccctgcc cgtcttcgag tccgttcccc
    14041 cgtccccccg cagggcgccc aagaccatcc acatcgcggc ctactcgatg cccacggaca
    14101 tcctcaccgt ctacccggac ggccagtact gggtcatcga cgacaagacc cgccgatccg
    14161 cggacgtcct gcgggacacc ctggcggagt tcaacatcga ggcggaggtc acggggatcc
    14221 gccggggtcc cgtggtcacg atgttcgaga tactcccggc cccgggggtc aagctctcca
    14281 agatcgtgaa cctctcggac aacatcgccc ttcgcctggc cgcctccagc gtccgcatcg
    14341 tggcgcccat cccgggcaag cacgcggtgg ggatcgaggt gcccaacgac aagcgctcgg
    14401 tggtgagctt ccgggagatc gtggagaccg acgtcttccg ggccgccaag ttcgagatcc
    14461 ccctggccct gggcaaggac atagccgggg aggtccagat catcgacctg acccagatgc
    14521 cccacctcct gatcgcgggt gcgacaggat cgggcaagtc cgtcctggtg aactccatca
    14581 tcctgtcgat cctgtaccgg aagagctgga acgaggtgaa gctcctcctc atcgatccca
    14641 agatcgtgga gctcaagctc tacaacggga tcccccacct cctcaccccc gtggtcacgg
    14701 agcccaagcg ggccttccag gccctccagt actgcatctg cgagatggag cggcgctaca
    14761 gcctc
//
LOCUS       DAOTEI010000081        14615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2843_length_14615_cov_4.330975, whole genome shotgun sequence.
ACCESSION   DAOTEI010000081 DAOTEI010000000
VERSION     DAOTEI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14615
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2843_length_14615_cov_4.330975"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /gene="hflC"
                     /locus_tag="P5313_09140"
     CDS             <1..936
                     /gene="hflC"
                     /locus_tag="P5313_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRY80569.1"
                     /translation="IVLVAAGPFYVVNEGEQAVVVRFGQIVSIERDAGLKFKTPFLDN
                     IVIYPKKILAWDGEPQRIPTLEKQFIWVDTTARWRISDPAKFYASVSSIDGAYGRMDN
                     VIDSAVRTVITQNYLREAVRNSNAIQQTRSSESFETGDAAGSEALGQLTQVEVSHESI
                     AKGRRALAQEMLALSRELTPEYGMEILDVQVRQIKYSDELTESVYNRMISERNQIAQA
                     FRSFGEGKKAEWLGKLENEKRSILSAAYTESETIKGRADAEATQIYSETYSRDPAFFA
                     FWRALESYKQTLPKFNKTLSTDMDYFRFLYGTRGR"
     gene            1085..2746
                     /locus_tag="P5313_09145"
     CDS             1085..2746
                     /locus_tag="P5313_09145"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY80570.1"
                     /translation="MSIRVKIVLVVLPILLVAIVLAGMSSYFVAARAVTRVATEFLDF
                     KASELEKYAESQWNLLVENGVADRPDMVAAAKAGVESFAVSILRSPTELILGVGTDGA
                     LTFRTSAAEFSPEETARAAEFHTRGVRGFFTLSVNGTDRVASGFPFGPFGWYVLVTED
                     RAAFYGDVETITRTTLGILAASLLIAAILLLLFVRYLTGPLTVVAGAMRRIIDSGDMS
                     ERVPVEYKDEIGQLSHTFNIMLEELAKAYENIKRYALSAKIAEIRERKTKNVFQLYVP
                     KHVLDEVLGNPEQALVGDNRVLPILFSDIRGFTTISESMRPDDLVNSLNRYFDVMVDI
                     IDARGGFVDKYIGDAIMAVFGAPVRHEDDALRAVQAGLEMVEALEVFNAEQVERGKPP
                     FNTGIGINYGVVTVGNIGCDKKMNYTVIGDSVNLASRLEGQTKTYHQPILISESAWMK
                     VRDTLPCRQIDKIAVKGKTIGVRVFTARKELSPEESRAWPLHEKGFELYYGRRFEEAA
                     RTFREVTSLLGSDDYASTFMAERCEAYAKSPPPSNWDGTEVLHEK"
     gene            2743..3969
                     /locus_tag="P5313_09150"
     CDS             2743..3969
                     /locus_tag="P5313_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80571.1"
                     /translation="MNRPGRFPFHPALLLAALSVLTPRLEAQDAASSGDLRPVAAVTS
                     VWTSPEAEPFAAPLASALLRELSGAGFKTEAHSGALNGTASDSALAAGLSSSVKARWA
                     AVARCEMDGRRLVWRASVYDALDGALIASDAQGAFPGLSALPLLDQSASAVAREAWAQ
                     RARVIPGEPLEYRLRFTSRNEGARVAFGTGEGAREAGIIENGELLAPFTAFRAGDPVV
                     VTVSGEGWWPRVSVFRPGPEDEILELPRLMPRADRMVSLGISTTRLLGAQAEYRHFLS
                     DDALFLRAADSLWLQYTFTPGSVPVLHNEVRLGAGTYLFLPRDSRFRMAVMTGVSGIL
                     TLAFPGDLADKFYFDATFDAFSLSLEWHTSGTWMFFLEQRYPYSLGLASGLLTRGWAQ
                     SDGPPMILTAGVMKRW"
     gene            3963..5015
                     /locus_tag="P5313_09155"
     CDS             3963..5015
                     /locus_tag="P5313_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80572.1"
                     /translation="MVAAPLRRRACAAALALFCAGFLSTCDFLTADIFPSWFSYVEAR
                     YDLRSAVSAFTPALASSDFFVVEYLETYSGNRVVAILIMGTTNKRLLLLHPDTLERLT
                     FIDNPEFSPFLAAASVGNEIVSGKVAVNLSTLSPTGTGPTVGLNGSKQGIMRTGPGGD
                     QFLICFVENVGPVYEIQRYKYSSLWAPSGSDIGTTWALPYPSSPWQMIDSETSESGIG
                     AMFLLNHATEAEQSAYLSYFPSTFTINTGTLDFLNDPAAQVTGPFRVDDGRAWITYDG
                     PVAYHRGDKGPDRLVRYKMGSGSASPYTPATELDSIPFDDNDELRVLSFDASGTWWFA
                     YDRRTGYLYKLRTWWK"
     gene            5012..6196
                     /locus_tag="P5313_09160"
     CDS             5012..6196
                     /locus_tag="P5313_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80573.1"
                     /translation="MKKDRFLRAAAAAAILVLSAVPALGDEEGIRILVRSAGAVGDAW
                     SASLVSALAGSALLDSVIPCPPGRDPAELAARLSCALAVDVESEAPAGKDAASRWRIL
                     DPLTYEALASGTITGPSPTARELAEYWWIPLVEAAEAALPKVKKTLVRVSAAPGTTVT
                     GLSEEPMVIPEEGFLDLPLRVPVTRPWRAVSKGAYPESGYFAALEQGQNLEIPRRPLR
                     LWTVEAGLTMLSFPEIWAHRNFGEDVYFIRFGMAQYLFGLYLVDEEYGEETPPAILSL
                     PLVMPGAGFGMYFLPSDAYVRPYGYASAFARILLLSGAFGFDTVAPIGTNAYLGAEWR
                     AFPRTAVFFEIGAAFYPFCDGFLMAASRGGQGNDGPYINLFGSRWFFEAPMIRFGTRF
                     TL"
     gene            6193..7320
                     /locus_tag="P5313_09165"
     CDS             6193..7320
                     /locus_tag="P5313_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80574.1"
                     /translation="MNNRCLRFRVSWGSTARTSVASRLGCAALAAAALALASCWHPAF
                     DPAISGSEAVIRKLGAPILWFSVEDVRAEESDELFFLPNAVYPPTVGLLLHFEAQRII
                     LSAIEFNSITHTGLYSSNSREFPRGLGEQFTVLPAPAGGSDAFFVTGILEGRYVHSMA
                     SPLDTSDTNLEIVNLPSLPGAAWPPTFGIGTVAKPLDTMARLTWIGYESGTLLRFYTD
                     LWNGGSAPGLGPVSDIQLPDEELISIPGKAVELESGHIFLSCGLIDGSRAIYRWNGSS
                     DPAPIRFSEIHGPLTGALSDSRLLAEKDGVLTVLDNNLKRLFSFPSGRLRFVHERWDG
                     TRMISVFSRAVFVRTESGDDIGRLKVEVYEIPTADLHKLAD"
     gene            complement(7460..9709)
                     /locus_tag="P5313_09170"
     CDS             complement(7460..9709)
                     /locus_tag="P5313_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80575.1"
                     /translation="MAEQGLLRKAQEIRAANGTGGIDLTLQDSAGISKEDQQDILVHI
                     DRVAQSSRILAGPDTWKVKPRKRGFAAPLLVNVLAVLVLAGGLYGLWKFFGAESSSQT
                     VSVAAISTAEGKLLQEIRREAEGRILEKDREITAIQEKMAALDREKSQLLSSVQDRVQ
                     AKETELRAELAAELEKERQRLTAAGLTPATIEERLKAFEQEKSAALKRELDEFARKAD
                     EERLAIQASLDKARTEFQASLQSATAERQRIQDESRRREQELRAQLDEQNKALEAERS
                     RTAESLATARTELTRYNEEAAQARSAEDRLLGLYSSARAAIQEGRVEDAARSAEAIRQ
                     YLADPALAKFTTLSRRREADLFAVDLVERAISSERARTSADSARVGDALAAMGRIREE
                     AALAKTAQEAGNRTEADAAYRRMLAALPELSAALAFLDENYTARAAGATLVQKDRLDQ
                     AWAALDAAYAAKDGPAFDAAFRNLLTAASLEEARAGLALQRAKEAGALALVASRRAAD
                     TQGAAVPLRAAAEDLAARRWLEALGGYSAVLARYPFAEQVPQALEDLRRSGRALADAF
                     QRYRTETEDRIRVLEGQLASVQGTSDIAADRAARTTAAGVSPAEFAAMREERDRLKID
                     LEVSQARYDAVTAAYTAYTAEEDSILSKGGDLALVAGRARMDQFLDNDAVTAALPGMR
                     ARISRYLEAFQAAGQKEVLFNAADIVDGAARIRDPAVRDRYFRDLEARYAGNPSMLEF
                     LQSMKESFR"
     gene            9997..10935
                     /locus_tag="P5313_09175"
     CDS             9997..10935
                     /locus_tag="P5313_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80576.1"
                     /translation="MTEKGSASELLSQAYERLREADLEGASTRLDQALSADFESEEVL
                     FALACAAWWKERLERLGGCPSPFERGEFVLSQWKGFLAFQSRKAGDFDAARYGFKRFA
                     FQTALREFRSLGPEDAESWEPELALRIGRCLKGSGDFDGALERLEPAARDRRDAPEVL
                     AELADVYALVNEARTSKALFREAFFLNPQRIDTAFLECPALVRLVEHIRSLGFRSPEL
                     EEWIPVYGSLLGVFSVKRELKPIEVGKLRQSIYELENEVKENAERRSLLTPRLINRYF
                     WLIDHYINAKEDRSRIDEVLLKIKLMDPAVHKQYVA"
     gene            10956..13643
                     /gene="greA"
                     /locus_tag="P5313_09180"
     CDS             10956..13643
                     /gene="greA"
                     /locus_tag="P5313_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRY80577.1"
                     /translation="MADSPIAKRLQEMLNEEKWTRATLSGYSVNHLKEIDALFKEAAD
                     AKAVDDVREVCEEHLGHTKNSIIALYLSGIIALSRQQIDDSNMVSLMDIFTENKKWNI
                     VEYICQRILDESGENRIALAKLAECYQNEDRVDEMYSVWERLIRVDFEEADIVKALAE
                     QREKEGRTEESIDFYRKALHRYIARGSFTAVREIWAKLVEACPEDIDFFLHVQRKIAK
                     QISEDKASGLLMDLYRHYKTKEDWNTATDILKVVIAYDEKNPAVRKEIVDCYRGKFAG
                     HSQLEDYIRLSNLGQSYRSLTEAIADFEKHIAFDVGNFVFHRTWNIGRIAAIRGDEIV
                     IDFAKKRGHSMSLKMAIDSLVTLSRDHIWVLKAVWSKDKLHDKVKTDPAWALRTVIKS
                     FDNRADLKKIKAELVPSILTSGEWTSWSAKARDILKTDPMFGNAQDSIGTFIVRDRSL
                     SMADKVYAQFKAEKGFFERVRFIRDLAAKGLTDSEYFMEMLGFFTGYLRSYSTVNEHI
                     MASYLLVKELAAEHKHLASALSPQFSELFANIEDPVSVYEGLKDSELRTSFLDHIKTF
                     VPDWAEVYVRLFPYSLSQKLVDILLNAGHTDSLQRMVIDIVENYKDHRDAFIWVVNNL
                     LDKPWVQELGLSYEKILITLIHILDISFKEIENHRDTTENRKINKQVQQILFKEGALE
                     TFLDESGHDTIERVYTLLDDVKDLDPALKLRLRKQIVEKFPDFKITGEAEKAVVSRGL
                     IVTSEKYSEKQKLLQQIMEVEVPTNQKEIAYALSLGDLRENAEYKAAKEKQDELNSKV
                     AKLKNEIERAQIFDKSTVNASKVSFGTIVHLVNETDGKEETYTILGPWESDPSSNVIS
                     YLSPLGKRLLNHKAGDRLSFAIHERKFEYLLKSIKAAQL"
     gene            13675..>14615
                     /locus_tag="P5313_09185"
     CDS             13675..>14615
                     /locus_tag="P5313_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY80578.1"
                     /translation="MKKLLLAFVVAVSLVSAVPAAAQEKPVDLIVLLDASQSMFPYFT
                     EVVDFVVSRIAREYLRFGDTFHLLTFTDSVRIEIAQAVRTEQDLKSLLGRLYLLYPFG
                     RSTDLVSALTSLYRYTADLPESSRKLIVMVTDGMHNPSAQSPNAALGADQVRASIEDT
                     TARIRANGWTIRIVRIPFEGTPGAAGAGTAGGTGTAEAPGVSPGAGDYLSEVATGLGA
                     PVTEFSSEGREDAAGRSVEMPRIAFPGHLGRQGYDFSFPLVISNPSDRDIRLQLSGVQ
                     IRGSNVLRKTLFTDVPKGAELTVHVAVRLPDSVEAGTV"
BASE COUNT         2570 a         4862 c         4487 g         2696 t
ORIGIN      
        1 atagtcctgg tggctgccgg tccgttctac gtggtcaacg agggtgagca ggccgtggtc
       61 gtgcgcttcg gccagatcgt ctcgatcgaa cgggatgcgg gcctgaagtt caagaccccc
      121 ttcctggaca acatcgtcat ctatccgaag aagatcctgg cctgggacgg ggagccccag
      181 cggatcccca ccctggagaa acagttcatc tgggtggaca ccaccgcccg gtggaggatt
      241 tccgaccccg ccaagttcta cgcctccgta tcctccatcg acggcgccta cggccgtatg
      301 gacaacgtca tcgattccgc ggtccgtacc gtaatcaccc agaactacct gcgcgaggcg
      361 gtccgcaatt ccaacgccat ccagcagacc cggagttccg agagcttcga gaccggggac
      421 gccgccggct cggaagccct gggccagctg acccaggtgg aggtctccca cgagtccata
      481 gcgaagggcc gccgggccct ggcccaggag atgctggccc tgtcccggga gctcactccc
      541 gagtacggta tggagatcct ggacgtccag gtccggcaga tcaagtactc agacgagctg
      601 accgaaagcg tgtacaaccg catgatcagc gagcgcaacc agatcgccca ggccttccgg
      661 agcttcgggg agggcaagaa ggcggaatgg ctgggcaagc tggaaaacga gaagcgttcc
      721 atcctgtccg cggcctacac ggagtcggag accatcaagg gaagggccga cgccgaggcc
      781 acccagatct actcggagac ctacagccgg gatcccgcat tcttcgcctt ctggcgggcc
      841 ctggagtcct acaagcagac cctgcccaag ttcaacaaga ccctgtccac ggacatggat
      901 tacttccggt tcctgtacgg gacccggggg cgctgagccg ggggacgatc cgggtgtttc
      961 caaggaaggg gcggttccgg cacggagccg ccccttcccc ttcatttgtc catcccgcaa
     1021 gaatttaaag agctatttcc tttcggaccc ttgaccggac cgcgtccgca tgtatagtga
     1081 tatcgtgagc attcgcgtca agatcgtcct ggtcgtcctt cccatcctcc tcgtggccat
     1141 cgtcctggcc ggtatgtcct cctacttcgt ggccgcccgg gccgttacgc gggtggccac
     1201 cgaattcctg gacttcaagg cctcggagct ggaaaagtac gcggaaagcc agtggaacct
     1261 cctggtggaa aacggggtgg cggaccggcc ggacatggtt gccgccgcca aggccggggt
     1321 cgagtccttc gcggtgtcca tcctgcggag ccccacggag ctgatcctcg gcgtcgggac
     1381 ggacggggcc ctgaccttcc gtacctccgc cgcggagttc tccccggagg agaccgcccg
     1441 ggccgcggaa tttcataccc ggggcgtacg agggttcttc accctctccg tgaacggtac
     1501 ggaccgggtg gcctcgggct tccccttcgg tcccttcggc tggtacgtcc tggtcaccga
     1561 ggaccgggcc gccttctacg gggacgtgga gaccatcacc cgtaccaccc tgggcatcct
     1621 ggccgcgtcc ctcctcatcg ccgcgatcct cctcctcctc ttcgtgcgct acctcacagg
     1681 ccccctgacc gtggtggccg gggctatgcg caggatcatc gactccgggg acatgtccga
     1741 gcgggtgccc gtggagtaca aggacgagat cggccagctt tcccatacat tcaacatcat
     1801 gctggaggag ctggccaagg cctacgagaa catcaagcgc tacgccctct ccgccaagat
     1861 cgcggagatc cgggaacgaa agacaaagaa cgtgttccag ctctacgtgc ccaagcacgt
     1921 cctggacgag gtcctgggca atcccgagca ggccctggtc ggcgacaacc gcgtcctgcc
     1981 catcctcttc tcggacatcc ggggtttcac gacgatctcc gagagcatgc ggcccgacga
     2041 cctggtgaac tccctgaacc gctacttcga cgtcatggtg gacatcatcg acgcccgggg
     2101 cggcttcgtg gacaagtaca tcggggacgc gatcatggcc gtgttcggcg ccccggtccg
     2161 gcacgaggac gacgccctgc gcgccgtgca ggccggcctg gagatggtcg aagccctcga
     2221 ggtgttcaac gcggagcagg tggaacgcgg caagcccccc ttcaacaccg ggatcgggat
     2281 caactacggg gtggtcacgg tcgggaacat cggctgcgac aagaagatga actacaccgt
     2341 gatcggggat tccgtgaacc tggcctcccg gctggaggga cagaccaaga cctaccacca
     2401 gcccatcctg atctccgaga gcgcctggat gaaggtgagg gacaccctcc cctgccgcca
     2461 gatcgacaag atcgcggtca agggaaagac catcggagtc cgggtcttca ccgcccggaa
     2521 ggagctgtcc ccggaggaat cccgggcctg gcccctgcac gagaagggct tcgagctcta
     2581 ctacggccgc aggttcgagg aggcggcccg gaccttccgg gaagtcacct ccctcctcgg
     2641 atccgacgac tacgcctcca cgttcatggc cgaacgctgc gaggcgtacg ccaagtcgcc
     2701 ccctccctcg aactgggacg gcacagaggt cctgcacgaa aaatgaacag acccgggcgc
     2761 ttccccttcc atcccgcgct ccttctcgcc gcgctctccg tcctcacccc gaggctcgag
     2821 gcccaggacg ctgcgtcctc cggtgacttg cgcccggtcg ccgcggtcac ttcggtctgg
     2881 accagcccgg aagccgagcc cttcgccgcc cccctggcct ccgccctcct ccgggaactg
     2941 tccggggcgg gcttcaagac cgaggcccat tccggagccc tgaacggcac ggcctccgac
     3001 tcggccctgg ccgcggggct ttcgtcctcc gtcaaggccc gctgggccgc ggtcgcccgc
     3061 tgcgagatgg acggccgccg cctggtctgg cgcgcctcgg tctacgacgc cctggacggg
     3121 gccctaatcg cctcggacgc ccagggggcc ttcccgggcc tctccgccct tcccctcctg
     3181 gaccagtccg cctccgcggt ggcccgggag gcctgggccc agcgcgcccg ggtcatcccc
     3241 ggggagcccc tggaataccg cctccgattt acctcccgga acgagggcgc ccgagtggcc
     3301 ttcggcaccg gggagggcgc ccgggaggcg gggatcatcg agaacgggga gctcctggcc
     3361 cccttcacgg ccttccgggc gggggatccc gtggtggtca ccgtcagcgg ggaaggctgg
     3421 tggccccggg tctccgtgtt tcgcccggga ccggaggacg agatcctgga actcccgagg
     3481 ctcatgcccc gggccgaccg catggtctcc ctcggcatct ccaccacgcg cctcctgggg
     3541 gcccaggcgg aatatcgcca tttcctgtcc gatgacgccc tcttcctccg ggccgcggac
     3601 tccctctggc tccagtacac cttcaccccc ggttccgttc cggtcctgca caacgaggta
     3661 cgcctgggag ccggcaccta cctcttcctg ccccgggact cccggttccg gatggccgtg
     3721 atgaccgggg tttccggcat cctcaccctc gccttcccgg gagacttggc ggacaagttc
     3781 tacttcgacg ccaccttcga cgccttctcc ctgtccctgg aatggcacac ctccgggacc
     3841 tggatgttct tcctggagca gcggtatccc tactccctgg gcctggcttc cggcctcctg
     3901 acccggggct gggcccagag cgacggccct cccatgatcc tgaccgcggg ggtgatgaaa
     3961 agatggtagc cgcacccctc cgccgccggg cctgcgccgc ggcgctcgcc ctcttctgcg
     4021 cgggcttttt gtccacctgc gacttcctga ccgcggatat tttcccgtcg tggttttcct
     4081 acgtggaggc gaggtatgat ctgcggtctg ccgtctccgc ttttacgccc gcgctagctt
     4141 cctccgattt tttcgtagtt gaatatctgg aaacctattc cggcaaccgc gttgtcgcaa
     4201 tacttataat gggaacaaca aacaagcggc tattattgtt gcacccggac accttggaac
     4261 gcttgacctt catcgacaat cccgagtttt cccccttcct cgccgccgcg tcggtaggga
     4321 acgagattgt cagtggcaaa gtagccgtca atttgtctac tctatcgccc actggtacag
     4381 gaccgactgt tggcctcaac ggatcgaaac aaggaatcat gcgcaccggc ccaggagggg
     4441 atcagttttt gatttgtttc gtcgaaaatg tcggaccggt ttacgaaatc cagcgataca
     4501 agtactcatc tctatgggca ccttcaggtt ccgatattgg cacaacctgg gcccttcctt
     4561 atccatcctc gccttggcag atgatcgata gcgagacaag cgagtcaggg atcggtgcga
     4621 tgttcctgct gaatcacgcc accgaagcag aacagtccgc ttatctttca tatttcccat
     4681 ccacattcac aatcaatacc gggaccttgg atttcttaaa tgatccggcg gctcaggtca
     4741 ccggcccctt ccgggtggac gacggccgcg catggatcac ctacgacggc cccgtggctt
     4801 accaccgggg cgacaaaggc ccggatcgtc tggtccgcta taagatggga agcggcagcg
     4861 catcgccgta tacaccggct acagaactcg actccatccc cttcgacgac aacgatgagc
     4921 tccgggtcct ttccttcgac gcctcaggca cctggtggtt cgcctacgac cggaggaccg
     4981 gatacctcta caagctgagg acctggtgga aatgaaaaaa gatcggttcc tccgggcggc
     5041 cgcggccgcg gcgatcctcg tgctttccgc ggtacccgcc ctgggcgatg aggagggaat
     5101 ccggatcctt gtccgttccg cgggcgcggt cggcgacgcc tggtcagcct ccctggtctc
     5161 cgccctggcc ggatccgccc tcctggactc ggtgatcccc tgtcctcccg gccgggatcc
     5221 cgcggagctg gcggcccggc tctcctgcgc cctggccgtg gacgtggagt ccgaagcccc
     5281 cgccgggaag gatgctgctt cccgctggag gatcctcgat cccctgacgt atgaggccct
     5341 ggcctcgggg acgatcacgg gcccttcccc caccgcccgg gaactggcgg aatactggtg
     5401 gatccccctg gtggaggccg cggaagccgc cctccccaag gtcaagaaga ccctggttcg
     5461 ggtgagcgcc gcaccgggca cgaccgtaac gggtctctcg gaggagccca tggtcatccc
     5521 ggaggagggt ttcctggacc tgcccctacg ggttccggtc acccggccat ggcgggccgt
     5581 ctccaagggc gcctatcccg agtccgggta cttcgcggcc ctggagcagg ggcagaacct
     5641 ggagatcccc cgtcggcccc tccggctatg gaccgtggag gccgggctta ccatgctgtc
     5701 cttcccggaa atctgggccc acaggaactt cggggaggat gtatatttca tccgcttcgg
     5761 catggcccag tacctcttcg gcctctacct tgtcgacgag gagtatggcg aggagacccc
     5821 gccggccatt ctctccctcc ccctggtgat gcccggagcc gggttcggca tgtacttcct
     5881 cccgagcgac gcctatgtcc gcccctacgg gtacgcctcc gccttcgccc ggatactgct
     5941 cctgagcggc gccttcggct tcgataccgt ggcaccgatc ggcacgaacg cctacctggg
     6001 ggccgagtgg agggccttcc cccggaccgc ggtgttcttc gaaatcggcg cggccttcta
     6061 ccccttctgc gacggctttt tgatggccgc ctcccgggga ggccagggca acgacggccc
     6121 ctacatcaac ctcttcgggt cgcggtggtt cttcgaggcg ccgatgatcc gtttcgggac
     6181 gaggttcacg ctatgaacaa ccggtgcctc cgttttaggg tatcctgggg atccaccgcc
     6241 cggacttccg tcgcctcgag gctcggctgc gccgccctgg ccgccgcagc cctcgccctg
     6301 gcctcctgct ggcatcccgc cttcgacccg gcgatttccg gctccgaggc ggtgatccga
     6361 aagctggggg cgccgatcct ttggttctcg gttgaggacg tgagagccga agagagtgac
     6421 gagttgtttt tccttccaaa tgcggtgtat ccgccgaccg ttggacttct cctccatttt
     6481 gaagcccaac ggatcattct tagcgcaatt gagtttaatt cgattaccca tacgggctta
     6541 tatagttcga acagccgcga gtttccccga ggtctgggtg aacagttcac tgttctacct
     6601 gctccggccg gcggatcgga tgcattcttc gttacgggca ttctggaagg tagatatgtg
     6661 cattccatgg ctagccctct ggacacgagt gatacgaatc ttgagattgt caatctgcct
     6721 tcccttcccg gcgctgcctg gccaccaacg ttcgggattg gtactgtcgc gaaaccccta
     6781 gatacgatgg cccggcttac ctggattgga tatgaatccg gtactttact gagattctat
     6841 acggatctct ggaatggcgg atccgcgccc ggactcggtc cggtgagcga catccagctt
     6901 ccggatgaag aattgatcag tattcccggc aaggcggtgg aattggaatc cgggcatata
     6961 tttctttcct gcggccttat cgacggttcc cgtgcgatct accgctggaa cggttcttcg
     7021 gaccctgctc caatccgctt ctctgagatc catggtccgc tcacaggcgc cctttcggat
     7081 tccaggctcc tcgcggagaa ggacggagtc ctgacggtcc tcgataacaa cctcaagcgc
     7141 ctcttctcct tcccctctgg caggctccgc ttcgtccatg aacgctggga tggtactcgg
     7201 atgatttccg tctttagccg ggctgtcttc gttcgaaccg aaagtggcga cgacatcggc
     7261 cgactcaagg tcgaggtgta cgagatcccc accgcggatt tgcacaagct ggcggactag
     7321 gagcctgtcg gacttgtgaa tttttagcgc cgataggtgc taaaaattca cgattatccg
     7381 gctcctttgg agtctgcaaa gtagaaaaat tgcatttttg cgtttttcta ttattaaagt
     7441 aggcaagcgc gacaaactcc tagcggaagc tctccttcat ggactgtagg aactcgagca
     7501 tggaggggtt cccggcgtac cgggcctcta ggtcccggaa gtagcggtcc cggacggcgg
     7561 gatcccggat ccgggcggcc ccgtccacga tgtccgcggc gttgaagagg acttccttct
     7621 gccccgcggc ctggaaggct tccaggtagc gggatatgcg ggcgcgcatt cccggcaggg
     7681 ctgccgtcac ggcgtcgttg tccaggaact ggtccatccg ggcccgcccc gcgaccagtg
     7741 cgaggtcgcc gccctttgaa aggatggaat cctcctccgc ggtgtaggcc gtgtacgcgg
     7801 ccgtaaccgc gtcgtaccgg gcttgggaga cctcaagatc gatcttcagc cggtcgcgtt
     7861 cctcccgcat ggccgcgaac tccgcggggg agactcccgc cgcggtggtc cgggcggccc
     7921 ggtcggccgc gatatcggag gttccctgga ccgaggcgag ctggccttcc aggactcgga
     7981 tccggtcctc ggtttcggtt cggtagcgtt ggaaggcatc ggccaaggcc cggccggacc
     8041 tgcgcaggtc ctccagagcc tggggtacct gctctgcgaa gggatagcgg gccaggaccg
     8101 cggaataccc gccgagggct tccagccagc gccgggccgc cagatcttcc gcggccgccc
     8161 gcaggggaac cgcggctcct tgggtgtccg cggcccgccg ggacgcgacg agggccaggg
     8221 cccccgcctc cttggccctc tgtagggcca ggcccgcacg cgcctcttcc agggaggcgg
     8281 cggtcaggag gttccggaac gcggcgtcga aggccggtcc gtccttggcc gcgtacgcgg
     8341 cgtccaacgc ggcccaggcc tgatcgagcc tgtctttctg gaccaatgtg gcccccgcgg
     8401 cccgggcggt gtagttctcg tccaggaatg ccagggccgc ggagagctcc ggaagggcgg
     8461 ccagcattcg gcggtatgcg gcgtccgcct cggtacggtt cccggcttcc tgggcggtct
     8521 tcgccagggc ggcctcctca cggatcctgc ccatggcggc cagggcgtcc cccacccggg
     8581 cggaatccgc ggaagtgcgg gcacgctccg aggagatcgc ccgctccacc aggtccacgg
     8641 cgaagagatc cgcctcacgg cgccgggaca gggtcgtgaa cttcgccagg gccggatccg
     8701 ccaggtactg gcgtatcgcc tccgcgctcc gggccgcgtc ctctacgcgg ccttcctgga
     8761 tggcggcccg ggcagacgag tagagcccca gcagacggtc ctcggcggaa cgggcctggg
     8821 cggcttcctc gttgtacctt gtaagctcgg tccgagcggt ggcaagactc tcggcggttc
     8881 gggaccgttc tgcttccagg gccttgttct gctcgtccag ctgggcccgg agctcctgct
     8941 cccgcctgcg ggactcgtcc tggatccgct ggcgttcggc ggtggcggac tgcaggctgg
     9001 cctggaactc ggtccgggct ttgtccaggc tggcctggat ggccaggcgc tcctcgtcgg
     9061 ccttgcgggc gaactcgtcc agttcacgct tcagggcggc ggatttttcc tgctcgaagg
     9121 ccttcaagcg ctcctcgatg gtcgcggggg tgagccccgc ggcggtcagc cgctgacgtt
     9181 ccttctcgag ctccgccgcc agctcggcgc gcaattccgt ctccttggcc tggacccggt
     9241 cctgcacgct cgaaaggagc tggctcttct cacggtccag ggcggccatt ttctcctgga
     9301 tggccgtgat ttcacggtcc ttttcaagga tccggccctc cgcctcccgg cggatctcct
     9361 gcaacagctt gccctcggcg gtggatattg ccgcgacgga tacggtttgg gaggagctct
     9421 cggcgccgaa gaatttccac aatccataca ggcccccggc gagcaccagg accgccagga
     9481 cgttcaccag caggggcgca gcgaagcccc gcttccgggg cttgaccttc caggtgtccg
     9541 gtcccgccag gatacggctg gactgggcga cccggtctat atggacaagg atgtcctgct
     9601 ggtcttcctt ggatatcccg gcgctgtcct gtagggtcag gtcgatcccc ccggtgccgt
     9661 tcgccgcgcg tatctcctgg gctttccgca acaatccttg ttcagccatc gatcgggctc
     9721 cttgttcctg gccggtacac gtacagagta taaaggattt cgctcccggt tttccatggt
     9781 tcgatggaaa gcgggacttc cggaatcccg ccggaacggc cggccggggt atcccgcatt
     9841 tgtattatcg gcaagatgtt atacggggaa tggggaatcc ggtcccggcc gctttcattg
     9901 actaacggtc cgggaatcgg ttaaaatggg tggatagaat cacgagcagg ggcggatgac
     9961 cgtccggccc gaaatccacg acctccgaaa gagaccatga cggaaaaggg cagcgcgagc
    10021 gaacttcttt cccaagccta cgaacgactc cgggaggcgg accttgaagg cgcctccacc
    10081 cgcctggacc aagccctgtc cgcggatttc gagagcgagg aagtcctgtt cgccctggcc
    10141 tgcgccgcct ggtggaagga gcgcctggaa cgtctgggcg gctgcccttc ccccttcgag
    10201 cggggagagt tcgtcctttc ccagtggaag ggcttcctgg ccttccagag ccgcaaggcc
    10261 ggggacttcg acgccgcccg gtacggattc aagcgcttcg ccttccagac cgcccttcgg
    10321 gagttccggt ccctggggcc ggaggacgcg gaatcctggg aacccgagct ggccctccgg
    10381 atcggacgct gcctcaaggg ttccggcgat ttcgacggcg ccctggagcg cctggagccg
    10441 gcggcccggg accgcaggga cgcgccggag gtgctggcgg aactggcgga cgtgtacgcc
    10501 ctggtgaacg aggcccggac ctccaaggcc ctgttccggg aagccttctt cctgaacccc
    10561 cagcgcatcg acacggcctt cctggaatgt ccggccctgg tccggctggt ggagcacatc
    10621 cggtccctgg gattccggag tcccgagctg gaggagtgga tacccgtcta cggaagcctc
    10681 ctgggggtat tttccgtgaa acgggagctg aagcccatcg aggtcggcaa gctccggcaa
    10741 tccatttatg aattggaaaa cgaggtaaag gaaaacgccg agcggaggag cctgttgacg
    10801 ccccggctga tcaaccggta cttctggctc atcgatcatt acatcaacgc caaggaagac
    10861 cgttccagga tcgacgaggt tctcctgaag atcaagttga tggacccggc cgtgcacaag
    10921 cagtacgtag cgtaaccgac ggagaggaga ccgatatggc agattcgccc atcgcgaaac
    10981 ggcttcagga gatgctcaac gaggagaagt ggacccgagc caccttgagc ggatactccg
    11041 tgaaccatct caaggagatc gacgccctgt tcaaggaggc cgcggacgcc aaggccgtgg
    11101 acgatgttcg cgaggtctgc gaggagcatc tgggccacac caagaacagc atcatcgcgc
    11161 tctacctttc cggcatcatc gccctctccc gccagcagat cgacgactcc aacatggtct
    11221 ccctgatgga catcttcacg gagaacaaga agtggaacat cgtggaatac atctgccagc
    11281 ggatcctgga cgagtccggc gagaaccgga tagccctggc caagctggcg gagtgctacc
    11341 agaacgagga ccgggtcgac gagatgtact cggtctggga gcgtttgatc cgggtcgatt
    11401 tcgaggaagc cgacatcgtc aaggccctgg cggagcagcg ggagaaggaa gggcggaccg
    11461 aggagtccat cgacttctac cgcaaggccc tccacaggta catcgcccgg ggctcgttca
    11521 cggcggtccg ggagatctgg gccaagctgg tcgaggcctg cccggaggac atcgacttct
    11581 tccttcacgt ccagcgcaag atcgccaagc agatcagcga ggacaaggcc tccggcctcc
    11641 ttatggacct ctaccgtcac tacaagacca aggaggactg gaacaccgcc acggacatcc
    11701 tgaaggtcgt gatcgcctac gacgagaaga atcccgcggt gcgcaaggag atcgtggact
    11761 gctaccgcgg caagttcgcg gggcactccc agctggagga ctacatccgg ctctccaacc
    11821 tgggccagtc ctaccggagc ctcacggagg ccatcgcgga cttcgagaag cacatcgcct
    11881 tcgacgtggg caacttcgtc ttccaccgga cctggaacat cggcaggatc gccgccatcc
    11941 ggggggacga gatcgtgatc gacttcgcca agaagcgcgg ccattccatg agcctcaaga
    12001 tggcgatcga cagcctagtg accctcagcc gcgaccacat ctgggtcctc aaggccgtgt
    12061 ggtccaagga caagctccac gacaaggtca agaccgaccc ggcctgggcc ctgcggacgg
    12121 tcatcaagag cttcgacaac cgggcggacc tcaagaagat caaggccgaa cttgtcccgt
    12181 ccatcctgac atccggcgag tggaccagct ggagcgccaa ggcccgggac atcctgaaga
    12241 cggatccgat gttcgggaac gcccaggact ccatcgggac cttcatcgtc cgggaccgct
    12301 ccctgtccat ggcggacaag gtctacgccc agttcaaggc cgagaagggc ttcttcgaac
    12361 gcgtccgctt catccgggac ctggccgcca agggtctgac ggattccgag tacttcatgg
    12421 agatgctcgg gttcttcacc ggctacctgc gctcgtactc cacggtcaac gagcacatca
    12481 tggcctccta cctcctggtc aaggaactgg ccgcggagca caagcacttg gcctccgccc
    12541 tttcccccca gttctccgag ctcttcgcga acatcgagga tccggtctcc gtgtacgagg
    12601 ggctcaagga ctccgagctt cggacttcct tcctggacca catcaagacc ttcgtgcccg
    12661 actgggccga ggtatacgtc cggctcttcc cctattccct ctcccagaag ttggtcgaca
    12721 tcctcctgaa cgcgggccat accgacagtc tccagcgcat ggtcatcgac atcgtggaga
    12781 actacaagga ccaccgggac gccttcatct gggtggtgaa caatctcctg gacaagccct
    12841 gggtgcagga gctcgggctt tcctacgaga agatcctgat caccctgatc cacatcctgg
    12901 acatctcgtt caaggagatc gagaaccacc gggacacgac ggagaaccgg aagatcaaca
    12961 agcaggtcca gcagatcctc ttcaaggagg gtgccctgga gaccttcctg gacgaatccg
    13021 ggcacgacac catcgagcgg gtctataccc tcctggacga cgtgaaggac ctggaccccg
    13081 cgctcaagct ccggcttcgg aagcagatcg tggagaagtt cccggacttc aagatcaccg
    13141 gcgaggccga aaaggccgtc gtatcccggg gcctcatcgt cacctcggag aaatactcgg
    13201 agaagcagaa actgctccag cagatcatgg aggtggaggt cccgaccaac cagaaagaga
    13261 tcgcctacgc cctctccctg ggcgaccttc gggaaaacgc cgagtacaag gctgccaagg
    13321 aaaagcagga cgaactcaac tcaaaggttg ccaagctcaa gaacgagatc gagcgcgccc
    13381 agatcttcga caagtccacg gtcaacgcgt ccaaggtttc cttcgggacc atcgtgcacc
    13441 tggtcaacga gacggacggc aaggaggaga cctacacgat cctgggtccc tgggagtccg
    13501 atccgtccag caacgtcatc tcgtacctct cgcccctggg caagcggctc ctgaaccaca
    13561 aggcggggga tcgcctttcc ttcgcgatcc acgagcgaaa gttcgagtac ctattgaagt
    13621 cgatcaaggc cgcgcagctt taaaacactc tgcccggccc gggaaggtgg accgatgaaa
    13681 aagctcctgc tcgcgttcgt cgtggcggta tccctggtct ccgcggtccc ggccgcggcg
    13741 caggaaaagc cggtcgacct catcgtgctg ctggacgcct cccagagcat gttcccgtac
    13801 ttcacggagg tggtggactt cgtggtgtcc cgcatcgccc gggaatacct ccggttcggg
    13861 gacaccttcc accttctgac cttcacggat tccgtgcgca tcgagatagc ccaggcggtg
    13921 cgcaccgagc aggatctgaa aagcctcctg ggcaggctgt acctcctcta ccccttcgga
    13981 cggagcacgg atctggtttc cgcccttacc tccctctatc gctataccgc ggaccttccg
    14041 gaatccagcc gcaagctgat cgtcatggtg accgacggga tgcacaatcc ctccgcccag
    14101 agccccaacg cggccctggg agcggaccag gtccgggcga gcatcgagga tacgacggcc
    14161 cggatccggg ccaacgggtg gaccatccgc atcgtgcgca tccccttcga gggaacaccc
    14221 ggagcggcag gtgccgggac cgccggtggt acgggaaccg cggaagcccc gggggtatcc
    14281 ccgggcgcag gggactacct gtccgaagtg gccaccggcc tgggcgctcc ggtcaccgag
    14341 ttttcctccg agggacggga ggacgcggcc ggccggagcg tggagatgcc ccgcatcgcc
    14401 ttcccggggc acctgggccg tcaagggtac gatttctcct tccccctggt gatatccaat
    14461 ccctccgacc gggacatccg tcttcagctc tcgggcgtcc agatccgagg atcgaacgtt
    14521 cttcggaaaa ccctgttcac ggacgtgccc aagggcgccg agctgacggt gcacgtcgcg
    14581 gtacgactgc cggactcggt ggaggcggga acggt
//
LOCUS       DAOTEI010000082        14587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2858_length_14587_cov_4.604734, whole genome shotgun sequence.
ACCESSION   DAOTEI010000082 DAOTEI010000000
VERSION     DAOTEI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14587
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2858_length_14587_cov_4.604734"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="P5313_09190"
     CDS             complement(<1..637)
                     /locus_tag="P5313_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HRY80579.1"
                     /translation="MGWSTVTGLYGTFVRPALDILILSFLVYKTWQILVRTQAVQLVK
                     GAVFMAVVYAASFFLRLDTLTWILNILAPGLVIALAIVFQPELRKIFMRIGQGSFFRA
                     GRRPRSTQLDSVLSAAEVLASRRRGALIVFSRSVGLKNIIDTGTRLGAEISSSLILTI
                     FGHDTPLHDGALVLAEGRIVAAGCFLPLSEQQDIRKSFGTRHRAALGLSEES"
     gene            complement(637..1458)
                     /gene="folP"
                     /locus_tag="P5313_09195"
     CDS             complement(637..1458)
                     /gene="folP"
                     /locus_tag="P5313_09195"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRY80580.1"
                     /translation="MGILNATPDSFWEGSRVSGAEAAARTALAMLRAGASILDIGGES
                     TRPGSAYLSAEEEIARVEPAIRAVRAAAAAEGLDPVLSVDTRKAAVARAALEAGADLV
                     NDVSALEDDPDLAGLCAERGVPVILMHKKGVPATMQNAPWYEDCAGEVRDYLLAAADR
                     ARAAGIPGDRILLDPGIGFGKRLEDNLDLLGRLAELRAHGYPVVVGLSRKSFLGALTG
                     RAVEERLAGSIAAACAAFLGGADVFRVHDVPETVDALKVFGAILGRTAAEGKGTP"
     gene            1746..2240
                     /locus_tag="P5313_09200"
     CDS             1746..2240
                     /locus_tag="P5313_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80581.1"
                     /translation="MRQTELKILRLHAPLRYAPAPISGGRPFPPRERPSEGAEDAALY
                     EPDSIVRFDPRDGPRALPSLPEPAAAGWAPASSAPHGTVRDTSGPGALCLEPGAYGFL
                     QGAAASEGEWLGLIEAFARQAWWEGTECEGPYIVRRVREDGRWAAQVWRRLAGESRDP
                     AGGG"
     gene            complement(2286..3683)
                     /locus_tag="P5313_09205"
     CDS             complement(2286..3683)
                     /locus_tag="P5313_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY80582.1"
                     /translation="MEIPAGVVLLSREGGQDLAGHLREAGFAVTVVSEPESVPPGVVI
                     LDLVRPEQAREWLHEKTDLPRILRARGNTVVAVVTPDTFEDDRNAIGVSGAHSYILRD
                     DPPRSVRATVLAAVSRRDQDERLRKSEERYRLLAENAGDVIWTWDLRAKAYEYISPSI
                     LRLRGLTVEEALAEDMRESMTPESYGRAMDALRAGLESLARGVRPAPITDKYDQPCKD
                     GSVKTVEITTSVIYDEAGAPTHVLGVSRDATERVRSQEALESALRRSELLFRELEHRV
                     KNTLAMIASLLSLASDHVRDPEDAVLFEESQSRIQALSLVYDRLLRSSDVARIELGSY
                     LEDLCRSVTSAFIRNAGGVDLRVDRDEVTVNSKRAALVGLAVNELVTNALKYARKPGA
                     VLSLGLSVRGSEDSLTVRFSDDGPGMPEGFRAEDAEGLGFLLIRSLVSQLGGEFTVET
                     GVPGAAFRITLPSET"
     gene            complement(3738..5528)
                     /locus_tag="P5313_09210"
     CDS             complement(3738..5528)
                     /locus_tag="P5313_09210"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRY80583.1"
                     /translation="MITIHELRGKYIEFFKSKGHAEISGKSLIPENDPTVLFTTAGMH
                     PLVPYLLGEPHPAGKRLTDYQKCIRTGDIDAVGDPSHLTFFEMLGNWSLGDYFKEEAL
                     AWSFEFLTDPKWLGIPKDKLSVTVFAGEEGIPRDDVSAEIWKKLGIPAERIHYLPRED
                     NWWGPAGETGPCGPDSEMFVDTGKSACGPDCRPGCHCGKYFEVWNDVFMQYNKDSEGR
                     YTPLKAPCVDTGMGIERTVAMLQGKKSVYDTEAFTDVLAAIGRACGKTYAAGDESGKS
                     FRIIADHARTSTFILGDPRAVTPSNVGAGYVLRRIIRRAVRHGRKLGIEGSFLPGIAE
                     VVIAVYRGPYPELVENRGRILEELAKEETKFLETLQKGEHEFEKMLPNLLKNPRKVMS
                     GRLAFKLYDTYGFPIELTEELAAESGMTVDRPEFDEAFKKHQELSRAGSEQVFKGGLA
                     DHGEAAVKYHTATHLLHKALRMVLGDHVAQKGSNITAERLRFDFSHPDPMTPEQIRKV
                     EEIVNEQIRRDLPVSMKVETLEEAKDEGAMALFGEKYGESVKVYTVGDFSKEVCGGPH
                     VEHTGVLGKFVIQKEQSSSAGVRRIRAVLE"
     gene            complement(5575..6807)
                     /locus_tag="P5313_09215"
     CDS             complement(5575..6807)
                     /locus_tag="P5313_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY80584.1"
                     /translation="MPLEHTDIQSIIQIDSELNKIRDYDLLLERILLEARRVVNADAG
                     SIYIKEGETLAIKYSQNDTKQQALPPGEKLIYSIFTIPVNEKTISGYVAKTGEFVNIP
                     DVYNLPPGCPFSYDTKFDTIAQYKTTSMMTIPLKTQNNVVLGVIQVINAKDPAGNVVP
                     FSQDDEILVTHFASNATVALQRAYLTRAMILRMIRMAEMRDPKETGAHVNRVAGYAVE
                     IYDRWAFKNGVPEKERDKQKDLLKLAAMLHDVGKVAISDVILKKPARFTEDEYKIMQT
                     HTLYGAKLFDDPQSDLDSVAAEVALTHHESWDGKGGYPGWVDPDTGKILKKRRDGSPR
                     TRKGEEIPLWGRIVSIADVYDALICRRVYKEPWDEDKVYSELRSLAGTKFDPSLIEIF
                     FEILPRIQQIRERNPDAE"
     gene            complement(6950..7975)
                     /locus_tag="P5313_09220"
     CDS             complement(6950..7975)
                     /locus_tag="P5313_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY80585.1"
                     /translation="MPTIKEIARKAGVSPTTVSNVLHGRTARVSSETRRRVEAFLKDE
                     EYAPNMGAVILARRNSRIIGVIVFMEPRRDETVLEDPFSSSILGAMEEEIRENGYFLM
                     LHATSDEDEVIRLARTWKLDGLVLLWVPAALCRAIRGSIETPVVFVDTYFEGKDRGFH
                     RIGLDDFQGGYRIADYVLSRGHRRVLFLANDDVHPGGDRFRFEGCRKAFADKGLEFGG
                     DRFVVVSKDPAERRELYGRLSRRPLEYTALLFSSDYYAAEAVSFLGDHGTRVPQDVSV
                     TGFDDNIFARLIRPRLTTIHQDVYGKGRAAVSMLMKLVRGIEPVETEIRLPVRLVERD
                     SVASAED"
     gene            complement(8009..9364)
                     /locus_tag="P5313_09225"
     CDS             complement(8009..9364)
                     /locus_tag="P5313_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRY80586.1"
                     /translation="MKIVLVGAGSAQFGHGTLGDIFCSGALEGSEIVLLDINAQSLEG
                     VLKTARDFIRKNDLKYTVSATTDRRTAFRGARFIISSIEVGNRFQLWDEDWKIPLQYG
                     FRQVYGENGGPGGVFHALRIVPVILEIVRDAVELCPEAWIFNYSNPMTAIVTTVKRAF
                     PEARFVGLCHEIGWLSRWLPRMLGMDERDLSFTAAGLNHFSCMIELTEAATGKDLYPE
                     VRKRAREFFLREPGYSDVYGDFLSTGVLTGHEQFEKEIADRTSRFEWADRRLVEFMLE
                     NYGLLPITTDSHFGEYLGWAWDVVDHRGILDFYDYYRAMLTQPVKPEIRLQTMERVVP
                     IIDGILGGTGFRESAVNVLNDGLLEDLPSWIAVEVPAVVDRRGVTGMKVSGLPKGYLA
                     LLRNYTGVYDLTAEAVIRGRKDLVVQALLANPVVHQVRGIRAMVDRMIDQQGRWLGYL
                     K"
     gene            complement(9421..10308)
                     /locus_tag="P5313_09230"
     CDS             complement(9421..10308)
                     /locus_tag="P5313_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY80587.1"
                     /translation="MTPSRTSPRTGRSGPAGIPALELITFLLFLLFMFPFALVVLNSA
                     KTSREIIFNAVAWPADWSQLFENVSLIFRNPTVDYLGAFADSTVITALSLVVIILFSS
                     MAAWVLVRNRTAWSTALFMAFVAAMVIPFQVLMYPLVRWMRVMGDFAGIRLLGTVPGI
                     VFGYLGFGAPLSIFVFHGFIKNIPYELEEAATIDGCGRARIFFEIVFPLLKPIIVTVM
                     ILNGIWIWNDYLLPLLVLGSNGVVQTIPIAVTAFAGAYLKQWDLILTSTLIAIIPVVL
                     LYLFAQRYIIKGMLEGSIK"
     gene            complement(10308..11195)
                     /locus_tag="P5313_09235"
     CDS             complement(10308..11195)
                     /locus_tag="P5313_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY80588.1"
                     /translation="MRKNTLVNVLFLAPCVFAFVMIIIIPFFLGLYYSMTDWNGVNPD
                     VAFVGLENFRSLFSAPDFIYSFLATMGYTLINVVLVNVVSFVLSLIVTSRIRFRNFFR
                     AGFFVPYLIGGIVLGYIWQFILNSILVDLGEALSLEFLQSSFLSRSGSVIWAMSAVNT
                     WQYAGYIMLIYIAAIQSIPASLMEAATVDGAGYLTRVFKILIPMMANAFTISLFLTLT
                     TSFKQFDMNFTLTNGGPATRFLDQPVKASQLLAMNIFNTATANRMAEAQAKAVVLFVS
                     LVVVALIQVSINKKKEVEM"
     gene            complement(11291..12571)
                     /locus_tag="P5313_09240"
     CDS             complement(11291..12571)
                     /locus_tag="P5313_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY80589.1"
                     /translation="MKKAVSTLAAAGLALAILMTASCAKKEAPKPQDVTVNMFQLKVE
                     IKDALDGYAASYSAAHPGVTVKVETLGGGGDYGGALKAKSQAGQMPDIFQIEGRGHYE
                     VWKDYIADLSAEPWVKDTDLAFKVDGKVLGFPVAIEGYGLAYNADILAKAGIDPASLT
                     TRAAYEKAFKTLDAKKKELGISAPVSMAASVAGGMWWVAGQHNLACYWGGGLPFDDTS
                     VIQKALKGEMDEPRFTQYAKYVQLLFKYADRNILLNGSYDDQVGAFAQGKTAFLHQGN
                     WVDPNLKQLGVTFKIGYAPHAFLDTEEKGLYLFAPSWYVVNSKSPNAQAAKAFLAAMA
                     TTPEGHDYMVNKAGMIPAFKSVTLKPSGQLSQELMAANARGGNYGVFFGMLPDGAGQN
                     VFAPVYDLFAQNPNNLSQFIADMKKAIAGLPGMK"
     gene            complement(12824..13672)
                     /locus_tag="P5313_09245"
     CDS             complement(12824..13672)
                     /locus_tag="P5313_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRY80590.1"
                     /translation="MSTTISKTHAADPDDSLRSYYGKIKTIPLLTAEEERELARRIRG
                     GDEEARGRLIEANLRLVVKIARTFSAYDVPLIDLIQEGNMGLIKAAEKFDDRRNVRFS
                     TYASWWIKQAITRSLVNSRRAIRLPHRKEELLKRIQRTYNALTQTLQTEPTSAEIARD
                     LRVSEESVNSVLEMSGSMVSLDAEDGDDSGSVIDIVEDTTYEPSRQLDEVCAREKVLR
                     MLELLLEKERRILLYRFEFFGGERYTLKQIGSELGISPETVRQIEKRALTKLREQASF
                     AEYAAI"
     gene            complement(13864..13992)
                     /locus_tag="P5313_09250"
     CDS             complement(13864..13992)
                     /locus_tag="P5313_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80591.1"
                     /translation="MRKLVIVVITIFAVFTFIREYPSEQRGPRTVQGSAVDSSAPN"
     gene            complement(14057..>14587)
                     /locus_tag="P5313_09255"
     CDS             complement(14057..>14587)
                     /locus_tag="P5313_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRY80592.1"
                     /translation="VGSVAARYGVGATAILDANDLGSSVIRPGQSLFIPGARMSATEL
                     KKALGELVAWPVRGSLSSPFGYRPNPFTGIRQFHNAIDIVAPEGTPVKAAMDGRVADT
                     GYNAIYGNYVILTHPDSYQTLYAHLSKVSVKRGTTVRQGAALGSVGTTGYSTGPHLHF
                     SVFKRGSAVDPLKLLK"
BASE COUNT         2485 a         4671 c         4817 g         2614 t
ORIGIN      
        1 cggattcctc cgagagcccc agggccgcgc gatgccgggt cccgaagctc ttgcggatgt
       61 cctgctgctc cgagaggggc aggaagcacc ccgcggccac gatccggccc tccgccagga
      121 ccagggcccc gtcgtgcagg ggggtgtcgt gcccgaagat cgtcaggatc aggctcgagg
      181 atatctccgc ccccagccgg gtccccgtgt cgatgatgtt cttcaggccc acgctgcggg
      241 aaaagacgat cagggctccg cgccgccggg acgccaggac ctccgccgcg ctcaggacag
      301 agtccagctg ggtggaccga ggacggcggc cggcccggaa aaagctgccc tggccgatcc
      361 gcatgaagat cttccggagc tcgggctgga agacgatggc cagggcgatg acgagccccg
      421 gggccaggat gttgaggatc caggtgaggg tatccaggcg caggaagaag gacgccgcgt
      481 agacgacggc catgaagacg gcgcccttga ccagctgcac ggcctgggtg cggaccagga
      541 tctgccaggt cttgtacacc aggaaagaga ggatgaggat gtccagggcg ggtcgtacga
      601 aggtcccgta cagcccggtc acggtgctcc atcccatcag ggagtaccct tcccttccgc
      661 ggccgtccgg cccaggatcg cgccgaaaac cttgagggcg tccacggttt ccgggacgtc
      721 gtggacccgg aatacgtccg cgcctcctag gaaggccgcg caggccgccg cgatcgatcc
      781 cgccaggcgc tcctccacgg ccctgcccgt cagggcgccc aggaaggact tgcgggacag
      841 gcccaccacg acaggatagc cgtgggcgcg caattccgca aggcgaccca ggaggtcgag
      901 gttgtcctcc aggcgcttgc cgaagccgat cccgggatcc aggaggatcc ggtccccagg
      961 tatgcccgcc gcccgggccc ggtccgcggc ggccaggagg tagtcccgga cctccccggc
     1021 gcagtcctcg taccaggggg cgttctgcat ggtcgcgggc acgcccttct tgtgcataag
     1081 gatgaccggg acgccccgct cggcgcagag gcccgccagg tccgggtcgt cctccagggc
     1141 ggagacgtcg ttgaccaggt cggccccggc ctccagggcg gcccgggcca cggccgcctt
     1201 ccgggtgtcc acggacagga ccgggtccag gccctccgcg gccgcggcgg cccggacggc
     1261 ccggatcgcg ggctccaccc gggcgatctc ctcctccgcg gacaggtacg ccgaacccgg
     1321 acgggtggac tccccgccga tatccaggat cgaggccccc gcccggagca tggccagggc
     1381 cgtccgggcc gcggcttccg ccccggagac ccggctgccc tcccagaagg aatccggtgt
     1441 cgcgttcagg atgcccatta cgtgccgggt ccccgccagg gccagggatc ggccgcgggg
     1501 aagggacagg atcgtcggaa cgacggacgg cggtttcagg tgcgtaccct ccttggcgtg
     1561 ggccggccgc gaaagccacc ttgaaatccg cgattgggag cagcgatgca gcgcaaccgc
     1621 aagcaatctc aaagccgatt taaaaagtga ccgtcttttg aaatcggctc aaagtataca
     1681 cactttcggg gccttcggaa cacccgggtg cttgcggaaa ccccgcatcc cgggcaggat
     1741 gggccatgcg gcagacggaa ttgaagatcc tgaggctcca cgcccccctg agatacgccc
     1801 ccgccccgat ctccggaggg cgtccctttc ccccaaggga acgcccttcc gagggagcgg
     1861 aggacgcggc cctttacgag cccgactcga tcgtccggtt cgatccccgg gacggtcccc
     1921 gagccctgcc ctccctgccc gaaccggcgg cggccggctg ggcgccggcc agttccgcgc
     1981 cgcacggaac cgtgcgggac acgtccggcc cgggcgccct gtgcctggag cccggggcgt
     2041 acgggttcct gcagggtgcg gccgcctcgg agggggaatg gctcggcctg atcgaggcct
     2101 tcgcccgaca ggcctggtgg gaagggacgg aatgcgaggg gccttacata gtccggcggg
     2161 tacgggagga cgggaggtgg gccgctcagg tctggagacg gctggccggg gagagtcggg
     2221 atccggcggg cgggggctga cggacccggg agcgccggcc gggcttctac ctcgggcccc
     2281 ggcgctcagg tctcggaggg cagggtgatc cggaaggccg ccccgggaac ccccgtctcc
     2341 acggtgaact cgccgcccag ctgggacacg aggctccgga tcaggaggaa tcccagcccc
     2401 tccgcatcct cggcgcggaa cccctccggc atgccgggcc cgtcgtcgga aaacctaacc
     2461 gtcagggagt cctcggagcc ccggaccgac agccccaggg aaaggacggc tccgggcttc
     2521 cgggcgtact tgagggcgtt cgtgacgagt tcgttcacgg ccaggccgac cagggccgcc
     2581 cgtttcgaat tgaccgtgac ctcgtcccgg tccacccgca ggtccacccc gcccgcgttc
     2641 cggatgaacg cggaggtcac ggagcggcaa agatcctcca ggtaggagcc gagctcgatg
     2701 cgcgcgacgt ccgaggaccg gaggagccgg tcgtagacca gggacagggc ctggatgcgg
     2761 ctctgggact cctcgaagag gaccgcgtcc tcgggatccc ggacatggtc ggaggccagg
     2821 gacagaaggc tggcgatcat ggccagggtg ttcttgaccc ggtgctccag ttcccggaac
     2881 aagagctcgc tccgccggag ggccgattcc agggcttcct ggctccggac ccgctccgtg
     2941 gcgtcccggg acacccccag gacgtgggtc ggggcgcccg cctcgtcgta gatcaccgag
     3001 gtggtgatct ccacggtctt cacggaaccg tccttgcagg gttggtcgta cttgtcggtg
     3061 atcggcgcgg gccggacgcc ccgggccagg gactcgaggc ccgcacgcag cgcgtccatc
     3121 gcccggccgt aggattccgg ggtcatggac tcccgcatgt cctccgcgag ggcctcctcc
     3181 acggtcaggc cccgaagccg caggatcgag gggctgatgt attcgtaggc cttggcccgg
     3241 agatcccagg tccagatcac gtccccggcg ttctccgcca ggaggcggta ccgctcctcg
     3301 ctcttccgga ggcgctcgtc ctggtcccgg cgactgacgg ccgccaggac ggtggcccgc
     3361 acgctgcggg gagggtcgtc ccggaggatg taggaatgcg ccccggacac cccgatggcg
     3421 ttccggtcgt cctcgaaggt atccggggtg accaccgcca cgaccgtgtt tccccgggcg
     3481 cgcaggatcc tcgggaggtc ggtcttctcg tggagccatt cccgggcttg ttcgggccgg
     3541 accaggtcca ggatcaccac ccccggcggg accgactcgg gttcggatac gacggtcacg
     3601 gcaaagcccg cttcccggag gtggcccgcg agatcctgcc cgccctcccg ggataaaagt
     3661 acaactcctg ctggaatctc cattccttca gtatcccggg aaaaagccgg attgtccaga
     3721 tggaatccgg gtcgggatta ctccaggacc gcccggatcc ggcggacccc ggcgctggag
     3781 ctctgctcct tctggatcac gaatttcccc agcaccccgg tgtgctccac gtggggtccc
     3841 ccgcagacct ccttggagaa gtccccgacg gtatagacct tcacgctctc cccgtatttt
     3901 tccccgaaga gggccatggc cccttcgtct ttagcctcct ccagggtctc caccttcatg
     3961 gagaccggga ggtccctgcg gatctgctcg ttcacgatct cctcgacctt ccggatctgc
     4021 tccggggtca tgggatcggg gtgggagaag tcgaaccgca ggcgctcggc ggtgatgttg
     4081 gaccccttct gggccacgtg gtcccccagg accatgcgga gggccttgtg cagcaggtgg
     4141 gtggcggtgt ggtatttgac cgccgcctcc ccgtggtccg cgagtccccc cttgaagacc
     4201 tgctcgctcc cggcccggga gagttcctgg tgcttcttga aggcctcgtc gaactccggg
     4261 cggtccacgg tcatcccgct ctccgcggcc agctcctcgg tcagctcgat ggggaagccg
     4321 taggtgtcgt agagcttgaa ggccaggcgg ccggacatga ccttccgggg gttcttgagg
     4381 aggttgggca gcatcttctc gaactcgtgc tcccccttct ggagggtctc caggaacttg
     4441 gtctcctcct tggccagctc ctccaggatg cggccccggt tttccaccag ctccgggtag
     4501 ggaccccggt agactgcgat cacaacctcc gcgatcccgg ggaggaagct cccctcgatg
     4561 ccaagcttgc ggccgtggcg cacggcccgg cggatgatgc gcctaaggac gtagcccgcc
     4621 cccacgttgg agggggtgac cgcccgggga tcccccagga tgaaggtgga ggtccgggcg
     4681 tggtccgcga tgatccggaa ggacttgccg gattcgtccc cggcggcgta ggtcttcccg
     4741 caggcccggc cgatggcggc caggacgtcc gtgaaggcct ccgtgtcgta cacgctcttc
     4801 ttgccctgga gcatggccac ggtgcgctcg atgcccatgc ccgtgtccac gcagggggcc
     4861 ttcaaggggg tgtagcgacc ctcggagtcc ttgttgtact gcatgaacac gtcgttccag
     4921 acctcgaagt acttgccgca gtggcagccg gggcggcagt ccggcccgca ggcggacttc
     4981 ccggtgtcca cgaacatctc gctgtccggg ccgcaggggc cggtctcccc ggcggggccc
     5041 caccagttgt cctcccgggg aaggtagtgg atccgctcgg cggggatgcc cagcttcttc
     5101 cagatctccg cggacacgtc gtcccggggt atcccctctt ccccggcgaa gaccgtcacg
     5161 gagagtttgt ccttggggat gcccagccac ttcgggtccg tgaggaactc gaagctccag
     5221 gccagggcct cctccttgaa gtagtccccc agggaccagt tgcccagcat ctcgaagaag
     5281 gtgaggtggc tggggtcgcc cacggcgtcg atgtccccgg tgcggatgca cttctggtag
     5341 tccgtgaggc gcttgccggc cggatggggc tcgcccagga ggtagggcac cagggggtgc
     5401 atgcccgcgg tggtgaacag gaccgtcggg tcgttctcgg ggatcaggga cttgccggag
     5461 atctcggcgt ggcccttgga cttgaagaac tctatgtatt tgccgcggag ttcgtggatg
     5521 gtgatcatgg ccgggagtgt agaacagccc cacggccctg gtcaaggttc ggggctactc
     5581 cgcgtcggga ttgcgctccc ggatctgctg gattcggggc aggatctcga agaagatctc
     5641 gatcagggaa ggatcgaact tcgtgcccgc cagggaccgg agttccgagt agaccttgtc
     5701 ctcgtcccag ggttccttgt agacccgccg gcagatgagg gcgtcgtaca cgtccgcgat
     5761 cgagacgatc cggccccaga gggggatctc ctcgcccttg cgggtccggg ggcttccgtc
     5821 cctgcgtttc ttcaggatct tcccggtgtc gggatccacc cagcccggat acccgccctt
     5881 cccgtcccag ctctcgtggt gggtcagggc cacctccgcc gctacggaat ccaggtcgga
     5941 ctggggatcg tcgaagagct tggccccgta gagggtgtgg gtttgcatga tcttgtactc
     6001 gtcctcggtg aaccgggcgg gtttcttcaa aatcacgtcg ctgatggcca ccttgcccac
     6061 gtcgtggagc atggccgcca gcttcaggag gtccttctgc ttgtcccgtt ccttctccgg
     6121 caccccgttc ttgaaggccc agcggtcgta gatctccacg gcgtacccgg ccacgcggtt
     6181 gacgtgggct cccgtctcct tgggatcccg catctcggcc atccggatca tccgcaggat
     6241 catggcccgg gtgaggtagg ctctctggag ggcgacggtg gcgttggagg cgaagtgggt
     6301 gaccaggatc tcgtcgtcct ggctgaaggg gaccacgttg ccggccggat ccttggcgtt
     6361 gatgacctgg atgaccccca ggaccacgtt gttctgggtc ttgaggggga tggtcatcat
     6421 ggaggtggtc ttgtactggg cgatcgtgtc gaacttggtg tcgtagctga aggggcaacc
     6481 cgggggcagg ttgtacacgt cggggatgtt cacgaactcc ccggtcttgg ccacgtaccc
     6541 ggagatcgtc ttctcgttca ccgggatcgt gaagatgctg tagatgagct tctcccccgg
     6601 ggggagggcc tgctgcttgg tgtcgttctg ggaatacttg atggcgaggg tctcgccctc
     6661 cttgatgtag atggagcccg cgtccgcgtt gacgacgcgt cgggcttcca ggaggatccg
     6721 ctccaggagg aggtcgtaat cccggatctt gttgagttca gagtcgatct ggatgatgga
     6781 ctggatgtcc gtgtgctcta gcggcatcgc tccccattcc gaaccccccg gggccgcacc
     6841 ccgggtgaga tgtacggcgt ctcccttaaa ggatcggccg caggaaggcc aagctgaaat
     6901 tgtaacggaa aaatgccgcc cgtgcaaatg gactttttgg aaataaaact caatcctccg
     6961 ctgaggccac ggaatcccgc tcgacgagcc tcacgggcag ccggatctcc gtctcgaccg
     7021 gctcgatccc ccggaccagc ttcatcagca tggacacggc cgcccgccct ttcccgtaga
     7081 cgtcctggtg tatcgtcgtc agccgggggc ggatcagccg ggcgaaaatg ttgtcgtcga
     7141 atcccgtgac ggaaacgtcc tgggggaccc gcgtgccgtg atcccccagg aacgagacgg
     7201 cttcggccgc gtagtagtcc gaggagaaca gcagggccgt atactccagg gggcgccggg
     7261 agaggcgtcc gtacagctcg cgccgctccg cgggatcctt ggagaccacg acgaaccggt
     7321 cccccccgaa ctccaatccc ttgtcggcga aggccttgcg gcacccctcg aaccggaacc
     7381 ggtctccccc cgggtgcacg tcgtcattag ccaggaacag gacccggcgg tgtccccggg
     7441 acagcacgta gtccgcgatc cgataccctc cctggaagtc gtccaaccct atcctatgga
     7501 atccccggtc cttcccctcg aaataggtgt ccacgaacac gaccggcgtc tcgatgctcc
     7561 cgcgaattgc cctgcacagg gcggcgggca cccacaggag caccagcccg tccagcttcc
     7621 aggtccgggc gagccggatc acctcgtcct cgtcgctggt cgcatgcagc atgaggaagt
     7681 agccgttttc gcggatctcc tcctccatgg cccccaggat gctggaggaa aacgggtcct
     7741 ccaggaccgt ctcgtccctg cggggttcca tgaagacgat cacgccgatg atccgggaat
     7801 tgcgtcgcgc caggatgacg gcccccatgt tgggagcgta ttcctcgtcc ttgaggaacg
     7861 cctccaccct ccggcgcgtc tccgaagaca cgcgcgccgt cctgccgtgc agcacgttcg
     7921 aaaccgtcgt ggggctgacg cctgcctttc gggctatctc cttgatggtg ggcatctcgg
     7981 ctccgtccgt tctaagcgga aggtccgcct atttcaggta tcccagccat cggccctgct
     8041 ggtcgatcat ccggtccacc atcgcgcgga tgccccggac ctgatgcacc acgggattgg
     8101 ccagcagggc ctggaccaca agatccttcc tgccccggat cacggcctcc gcggtcaggt
     8161 cgtagacccc cgtgtaattc cggagaaggg ccaggtatcc cttgggcagg ccggaaacct
     8221 tcatccccgt cacgccccgc cggtccacga cggccggtac ctccacggcg atccaggaag
     8281 gcagatcctc cagcaggccg tcgttgagca cgttcaccgc ggattcccgg aaaccggtcc
     8341 cccccaggat accgtcgatg atcgggacga ccctctccat ggtctggagg cggatctccg
     8401 gcttcaccgg ctgggtcagc atcgcccggt aatagtcgta gaagtccagg atgccccgat
     8461 ggtccaccac gtcccatgcc caacccaggt attccccgaa gtggctgtcc gtggtgatcg
     8521 ggaggagccc gtagttctca agcatgaact ccacgagcct acgatccgcc cattcgaatc
     8581 ggctcgtgcg gtccgcgatc tccttttcga actgctcgtg gccggtcagg accccggtac
     8641 tcaggaagtc tccgtatacg tcgctgtagc ccggctcgcg aaggaaaaac tcccgggcgc
     8701 gcttcctcac ctcgggatac aggtccttgc cggtcgcggc ctcggtaagc tcgatcatgc
     8761 agctgaagtg gttgagcccg gcggcggtga agctcaagtc cctctcgtcc atgcccaaca
     8821 tccggggaag ccatcgggaa agccagccta tctcgtggca gagccccacg aaccgcgcct
     8881 cggggaacgc ccgtttcacc gtcgtcacga tggccgtcat ggggttggaa tagttgaata
     8941 tccaggcctc cggacagagc tccacggcgt cccggactat ctccaggatc acggggacga
     9001 tgcgaagcgc gtggaacacc cctccgggcc ccccgttctc gccgtagacc tgccggaacc
     9061 cgtactggag ggggatcttc cagtcctcgt cccagagctg gaaccggttg cccacctcga
     9121 tggaggagat gatgaatcgg gccccccgga aggcggtccg gcggtccgtc gtcgcgctta
     9181 ccgtgtactt gaggtcgttc ttgcggatga aatcccgggc cgtcttgaga accccctcca
     9241 gggactgcgc gttgatgtcc aggaggacga tttcgctacc ctccagggcg ccgctgcaga
     9301 agatatcccc gagggtcccg tggccgaact gggcgcttcc cgccccgacc agtacgattt
     9361 tcatggcttg atgctccttg accttcccgg cccaccgtgg gcggcgggcc gcgacgtcgg
     9421 ctattttatg gaaccttcca gcatgccctt gatgatatag cgctgggcga acaaatatag
     9481 aaggacgacc gggatgattg caatgagggt cgacgtgagg atgagatccc attgcttcag
     9541 gtaggcgccc gcgaaggccg tcaccgcgat gggtatcgtc tgaaccaccc cgttcgatcc
     9601 gagcacgagc aggggtaaaa ggtaatcgtt ccagatccag atcccgttca ggatcatgac
     9661 cgtcacgatg atgggcttga gaaggggaaa cacgatttcg aagaagatgc gggcccgtcc
     9721 gcaaccgtcg atggtcgcgg cttcctccag ctcgtagggt atgttcttga tgaaaccgtg
     9781 gaacacgaag atcgacagcg gcgccccgaa gcccaggtac ccgaagacga tgcccggaac
     9841 ggtccccaga agccggattc cggcgaaatc ccccatgacc cgcatccatc gcaccagggg
     9901 atacatgaga acctggaagg gtatgaccat ggccgccacg aaggccatga acagggccgt
     9961 cgaccaggcc gtccggttcc ggacgagcac ccaagccgcc atggaggaga acaggatgat
    10021 caccaccagg gacagggccg tgatgaccgt gctgtccgcg aaggccccga ggtagtcgac
    10081 cgtgggattc cggaaaatga gggagacgtt ctcgaaaagc tgactccaat ccgcgggcca
    10141 ggcgacggcg ttgaaaatga tctcccggga agtcttggcg gagttcagga ccacgagcgc
    10201 gaaggggaac atgaacagca ggaacagcag gaaggtgatg agctcgaggg cggggatgcc
    10261 cgccggtccg ctcctcccgg tccgcggcga cgttcgcgag ggggtcatca catctccacc
    10321 tctttcttct tgttgatgga gacctggatc agggccacga caaccaggga gacgaacaat
    10381 acgaccgcct tggcctgggc ctccgccatg cggttggcgg tcgccgtatt gaagatgttc
    10441 atcgccagaa gctggctggc ctttaccggc tggtccagga acctggtggc gggccccccg
    10501 ttcgttagcg tgaagttcat gtcgaactgc ttgaacgagg tcgtcaaggt gaggaacagg
    10561 gatatcgtga aggcgttggc catcatgggg atgaggatct tgaagacccg ggtgaggtac
    10621 ccggccccgt cgacggtcgc cgcctccata agggaagccg gtatgctctg gattgccgcg
    10681 atatagatta gcatgatgta tccggcatac tgccaggtgt tcaccgcgga catggcccag
    10741 atgacgctgc ccgaccggct gaggaaggag gactggagga actccaggga tagggcctcg
    10801 cccaggtcga ccaggatgct gttcaggatg aactgccaga tgtagcccag gacgatgccg
    10861 ccgatcaggt agggcacgaa gaaccccgca cggaagaaat tccggaaccg gatccggcta
    10921 gtcacgatga gggaaaggac gaaactgacc acgttgacca ggacgacgtt gatgagggta
    10981 taccccatgg tggccaggaa ggagtagatg aagtccgggg ccgagaacaa gctccggaaa
    11041 ttctcgagcc ccacgaacgc cacgtccggg ttgaccccgt tccaatcggt catggagtaa
    11101 tagaggccca ggaagaacgg gatgatgatg atcatcacga aggcgaagac gcacggcgcg
    11161 agaaagagaa cgttcaccag ggtattcttg cgcatcaagg tgccctcctt cgcttcctcc
    11221 gtcggttccc ggaggccggg gaccgaccgg cgggttcgat cgtcccgccg tcggcctccc
    11281 ggacggatgc tcacttcatg ccgggcaatc cggcgatcgc cttcttcatg tccgctatga
    11341 actgggagag gttgttcggg ttctgcgcga acaggtcgta cacgggcgcg aagacgttct
    11401 ggccggcgcc gtcgggaagc atcccgaaga agacgccgta gttgccgccg cgggcgttgg
    11461 ccgccatcag ttcctggctg agctggccgc tgggcttgag ggttacggac ttgaacgcgg
    11521 ggatcatgcc cgccttgttg accatgtagt cgtgcccttc cggcgtggtc gccatggcgg
    11581 ccaggaaggc tttcgcggcc tgggcgttcg ggctcttgct gttcacgacg taccagctgg
    11641 gggcgaacag atacaggccc ttctcctcgg tgtccaggaa cgcgtggggc gcgtatccga
    11701 tcttgaaggt cacgcccagc tgcttgagat tgggatccac ccagttcccc tggtggagaa
    11761 acgccgtctt gccctgggcg aacgccccga cctggtcgtc gtagctgccg ttcaggagga
    11821 tgttccggtc cgcgtatttg aagaggagct gcacgtactt ggcgtactgg gtgaaccggg
    11881 gctcgtccat ctcccccttg agggccttct ggatcacgct ggtgtcatcg aacggcagcc
    11941 cgccgcccca gtagcaggcc aggttgtgct ggccggccac ccaccacatc ccccccgcca
    12001 cggaggcggc catggaaacg ggggcggata tcccgagctc ctttttcttc gcgtccaggg
    12061 tcttgaaggc cttttcgtag gccgcgcgcg tcgtcaggga cgcgggatcg atgcccgcct
    12121 tggccaggat gtccgcatta tacgcgagcc cgtatccctc gatggcgacg gggaatccca
    12181 ggaccttgcc gtccaccttg aaggccaggt cggtgtcctt gacccagggt tcggcgctta
    12241 gatccgcgat gtagtccttc cagacctcgt agtgcccccg gccctcgatc tggaagatgt
    12301 ccggcatctg gccggcctgg gacttggcct tcaacgctcc gccgtaatcg ccgcccccgc
    12361 ccaaggtctc taccttgacg gtgacgccgg ggtgggccgc cgaataggac gccgcgtagc
    12421 cgtccagggc gtccttgatc tcgaccttga gctggaacat gttgacggtc acgtcctgag
    12481 gtttcggagc ttccttcttg gcgcaggagg ccgtcataag tatggcaagc gccagccctg
    12541 ccgctgcgag cgtggaaacc gctttcttca ttttcctccc ccttttggtc ctttcgggcc
    12601 tgcatggccg cgtccgcgaa ttcgcggtcc acgggtaggt gtttcgcaac acctgtttca
    12661 attgtagcac caggcccggg ggtgtcaaga aaaagaaggc cgtatcggga agaatcggcg
    12721 tatatcttcc ctacggacca ggaaggaaag tcgagattgg gaataatgga gatccggact
    12781 ccgggttcgg actctctagc ttctccggga aatgcggagc cgctcagatc gcggcgtatt
    12841 ccgcgaagga ggcctgctcc cggagcttgg tgagggcgcg cttctcgatc tgccgcaccg
    12901 tctccggaga gatccccagc tccgagccga tctgcttgag ggtgtagcgc tctccgccga
    12961 agaactcgaa gcggtagagc aggatgcgcc gctccttctc caggaggagt tccagcatcc
    13021 gcagtacctt ctcccgggcg cagacctcgt ccagctggcg ggagggttcg taggtggtgt
    13081 cctccacgat gtcgatcacc gagccggagt cgtcgccgtc ctcggcgtcc agggagacca
    13141 tgcttcccga catctccagc acggagttga cggactcctc ggacacccgg aggtcccggg
    13201 cgatctcggc gctggtgggt tcggtctgga gggtctgggt gagggcgttg taggtccgct
    13261 ggatccgctt gaggagctcc tccttgcggt ggggaagacg gatggcccgg cggctgttga
    13321 ccagggaccg cgtgatggcc tgcttgatcc accaggacgc gtaggtggaa aaccgcacgt
    13381 tccggcggtc gtcgaacttc tcggcggcct ttatgaggcc catgttgcct tcctggatca
    13441 ggtcgatgag ggggacgtcg taggcggaga aggtccgggc gatcttgacg accagccgga
    13501 ggttggcctc gatcaggcgt ccgcgggcct cctcgtcccc tccccggatc cgccgcgcaa
    13561 gctcgcgctc ctcctccgcc gtgaggaggg gaatggtctt gatcttgccg tagtacgagc
    13621 ggaggctgtc gtcgggatcc gcggcatggg tcttgctaat cgtggtactc atcgtaccag
    13681 tatatatgca ggttgcgtgc caagttttat tcgacaggtg agccgaaaac atttctttgt
    13741 atggaataga gttatcgtct atatatcacg gagggggcag gaattcaaga aaaaccgtat
    13801 gtttttttag actttaggcg ttcacgggcg tataaaaaag tacaagggtg ggggggccct
    13861 gggtcaattc ggagcggagg agtcgacggc ggaaccctgg acggtgcggg ggccccgctg
    13921 ttccgacggg tattcgcgga tgaaggtgaa tacggcgaag atcgtaatga ccacgatgac
    13981 cagtttccgc atacttcctc ccgaaccgta tcgcatacta ccgtcgaagc gcccgcacgt
    14041 caatgaagga acagggctac ttgaggagtt taagggggtc cacggcgctc ccccgcttga
    14101 ataccgagaa atgcaggtgg ggtccggtgc tgtatcccgt ggtgcccacg gatcccaggg
    14161 cggccccctg ccgcaccgtc gtcccgcgct tcacggaaac cttgctcagg tgggcgtaga
    14221 gggtctggta ggaatccggg tgggtcagga tcacgtagtt gccgtagatc gcgttgtagc
    14281 ccgtgtccgc gacccggccg tccatggcgg ccttgacggg ggtaccctcg ggggcgacga
    14341 tgtcgatggc gttgtggaac tggcggatcc ccgtgaaggg attcgggcga tacccgaagg
    14401 gcgagctcag ggaaccccga accggccacg ccaccagctc ccccagggcc tttttaagct
    14461 ccgtcgcgga catccgggct ccggggatga agagggactg gccgggccgg atcacggagg
    14521 atcccaggtc gttggcgtcc aggatggccg tagctccgac cccgtaccgg gccgccacgg
    14581 aacccac
//
LOCUS       DAOTEI010000083        14390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2908_length_14390_cov_5.572375, whole genome shotgun sequence.
ACCESSION   DAOTEI010000083 DAOTEI010000000
VERSION     DAOTEI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_14390_cov_5.572375"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="P5313_09260"
     CDS             complement(<1..419)
                     /locus_tag="P5313_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRY80593.1"
                     /translation="MAKFGLGKGLGALIPESEVEVRLEALEGPEGEHRPPVPEGLRSV
                     PLGRLVPNPLQPRKTFDEASLEELAASIRLHGIIQPVIVEDSGDGTYLIVAGERRYKA
                     AEKAGLREVPVVVRSFTPEKKLEIALIENVQREDLNPV"
     gene            complement(412..1167)
                     /locus_tag="P5313_09265"
     CDS             complement(412..1167)
                     /locus_tag="P5313_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY80594.1"
                     /translation="MARTVVFVNQKGGVGKTTSAINLGAYLAKAGKRVLLVDFDPQAN
                     LTSGVGGKTGSRGIYEVLSGALALSDVIVPTKVEGLDLAPSSIELSGATVELVDKEDR
                     NDYLKKALRPVLDRYDFVLIDCPPSLGILTLNGLNAADEVLVPLQCEYFALEGLTLIL
                     QTVSLVQKSMNPRLRIGGILFTMYDSRTKLAQQVVQQVTQYFKDRVFRTIIPRNVRLS
                     EAPSHGVPVCLYDPDCVGAKSYERLAGEILSRG"
     gene            1393..1617
                     /locus_tag="P5313_09270"
     CDS             1393..1617
                     /locus_tag="P5313_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRY80595.1"
                     /translation="MLKEKVQKAIQDVRPSLQADGGDIELISVSDDGLVKVRLKGACG
                     SCPMATMTLKNGVENYLKKVIPEVKEVVQG"
     gene            complement(1948..2136)
                     /locus_tag="P5313_09275"
     CDS             complement(1948..2136)
                     /locus_tag="P5313_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80596.1"
                     /translation="MADKAYKCKDCGKEKIVKDTNSAPDCCGHEMQQIPMDLCGKAHS
                     AETARFNQPDDACDDGVR"
     gene            complement(2226..3365)
                     /gene="cheB"
                     /locus_tag="P5313_09280"
     CDS             complement(2226..3365)
                     /gene="cheB"
                     /locus_tag="P5313_09280"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HRY80597.1"
                     /translation="MPDPVNVLVVDDSALMRNLVSKMIEATPGLKVADRAMNGLFALQ
                     KIPRCNPDVIVLDIEMPEMNGIEFLKERRRLGIEIPVVVLSAVAKKGAEVTMEALALG
                     AADFITKPSGSVSSDIHTVAGELARLLLAYGTQYQKSRGKTPAAYPFESPELMLEKEP
                     GTSAPPPPAARRSPKPAEKPVPARSPGPVDLVAIGISTGGPNALRQVFARIDPRLVPP
                     VVVVQHMPPGFTEEFARSLDRICPLDVKEAADGDLLKPGRVLIAPGDKHLLVEKKSLA
                     AVARLSDAPPENGHRPSADVLFASVAREYGNRALGVIMTGMGRDGARELGALFREGGI
                     TLGQDEASSVVYGMPKVAFELGHVTEQVPLERMADRISELARLNR"
     gene            complement(3430..4251)
                     /locus_tag="P5313_09285"
     CDS             complement(3430..4251)
                     /locus_tag="P5313_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRY80598.1"
                     /translation="MSDFLPDAEFELYRKLIYDESGIHFSATNRSILESRLRERLREK
                     MTDSLRDYFNLITRDREELKILLDSVTTNLTRFFRNQPHFDALEKFVVPELIRIRGTS
                     PDRKLRIWSAGCSTGEEPYTIALLLREILPAGWTCEIVASDISLKSLMVGKEGFYPDS
                     RIVGIPEAYLPKYFERKPNGWQIREDVKKLVRFDYHNLKNDSGLRNMDVVFCRNVLIY
                     FDEVAQKAAVERFYETMGPKSFLFIGHSESLFGMNTRFEFVKTEWACFYKKDVKP"
     gene            4580..5086
                     /locus_tag="P5313_09290"
     CDS             4580..5086
                     /locus_tag="P5313_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRY80599.1"
                     /translation="MATEDVVLSVRGVAAGFGDEAPLVVLQDEPRSSSLRIPVGPYEA
                     SSIILEVEGIAPYRPLAHDLLATFFRDHGFLLESARIYDAIPDGDGGERFLSRIRYRK
                     GLRKWEKEVRPSDAIALALRLGAPILAPREFLDRREARRAAAAGDTRLPLYLQVPRSE
                     AAGSRSDR"
     gene            5136..6779
                     /locus_tag="P5313_09295"
     CDS             5136..6779
                     /locus_tag="P5313_09295"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HRY80600.1"
                     /translation="MNIFSSLVEFLRGILGRGGPDAAKKAELRKIYAYLSQIRPPYYK
                     PKGNLVLPGFAQGVYNFRAILRPLVDMARKTISHSDIRIYQRFFDLLVDARLPEETRQ
                     RKASFAYEGMKARIANAVKEDEEFEIIGREFQDFLHQVDSLSIGDLDRELAEVEQFSD
                     ICRFDWDRIIGLFDPGANMDNPGYRPDFRPAEGEKVLPELVDLYYILGDFAFEEGLAQ
                     NLLIILERQAPQTPVSTQRQKLDKIFAALNKGLALRLHRDVLLALIRALKSDPVYDPP
                     LVRETNDYVERYRRRMVTQYEKDRERLQREQHENAISTDIDALFGGTEVAPVDGYDEE
                     EDAYLRRESPNGFTHIKPLRILKTFVHNTFEKEIKEPVKRILVEGYFDNKGFQNNLAN
                     IIYQCERSAGRIDEFEASLRGNGRISIVAMKRYVEEMRRGKDVSQFLNRLVDAINQRA
                     REICEDETRLLQMLEESMGDLLTDYRRPSPDLVTNIRTLGGARNKEILGQVAEGRRHL
                     DTLLRIMRNFTYIRAPGTPPGTPGVAVEPPGLAEAEPAP"
     gene            6809..8233
                     /gene="purF"
                     /locus_tag="P5313_09300"
     CDS             6809..8233
                     /gene="purF"
                     /locus_tag="P5313_09300"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRY80601.1"
                     /translation="MLETPVESHDDSSLGHYCGVAGIRSPSPLDVPSALFYMLFALQH
                     RGQESAGIVWAEGGAHRADRRLGMVSAAADAWLSGRAASTGGIGHVRYSTAGGGGIRN
                     AQPLAVECSKGSVALAHNGNISNSAALRAALTREGAIFQSSSDSELILHLISRSRAEG
                     IEAILREALAPLEGAYSLCMLWGETLVAVRDPWGFRPLHLAEKDGVRYVASETCALHA
                     LGPSISQREVLPGEAVIVDDHGERSFFLPAAPRRARCVFELLYFARPDSRVFGESVHA
                     ARERFGEALAEADDVEADLVVPVPDSGNLAALGYARRAGIPFEFGITRNHYAGRSFIL
                     PTRAEREFAVRMKLHPVPEIVGGKRVVLVDDSLVRGTTSRLIVGLLKEAGAREVHLRL
                     SAPELTGPCGYGIDIPDRSELISNRMSPAEIARYVGADTVRFLPPDLLRSRLAEPSEY
                     CYACFDGDYPCPIRNGEGKETPCP"
     gene            8224..9186
                     /gene="purM"
                     /locus_tag="P5313_09305"
     CDS             8224..9186
                     /gene="purM"
                     /locus_tag="P5313_09305"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRY80602.1"
                     /translation="MPVDSYKKAGVDIAAGDAFADFIRDLPSKAVSKAIGGFAGGVPL
                     DLSGWKEPMLLSTTDGVGTKLLVAKKLGRWDSIGIDLVAMSVNDLAVCGADPISFLDY
                     IACGRIREEVLRDVIRGVVAGCEQADCVLAGGETAEMPDVYGPDDLDLAGFCVGVVEK
                     SGMLPLLSRIEAGDAVLGLPSSGVHSNGYSLARKCVPEDDRTAWEELLTPTRIYVREL
                     RLLRETGKLLAAAHITGSGLEANFERVIPKSLKARFTWDWPRPGIFDRIQRGGNVPEE
                     EMRRVFNLGVGIAFVVKPADADSTLALCRDRGIPCFPLGELVRA"
     gene            9179..9769
                     /gene="purN"
                     /locus_tag="P5313_09310"
     CDS             9179..9769
                     /gene="purN"
                     /locus_tag="P5313_09310"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRY80603.1"
                     /translation="MPNLAVLASGSGSNFEALARALEPTEHECVLLVCDRAGAGAFER
                     AALLGVPARHVSYTGRPRAEAEAEIEALLSEAGSDLVALAGFLRLLSPDFVRRRAGRI
                     LNVHPALLPAYPGLDALRRAWDAGEEVFGATIHLVDEGMDTGPVLRQGNLKRRPGESF
                     EDLEARVHALEHELYRRTVLELLDGIAREGSPGGRR"
     gene            9766..11013
                     /gene="purD"
                     /locus_tag="P5313_09315"
     CDS             9766..11013
                     /gene="purD"
                     /locus_tag="P5313_09315"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRY80604.1"
                     /translation="MNVLVIGSGAREHALARRLGTEGNRVSCAPGNAGTAGIADNLPL
                     NPDDAETVLRACRELGADLVVIGPEDPLAAGLADRLRDAGYPVFGPGARSARLESSKD
                     FARAFAERHGVPRARTEVLRTESDLRDYIGRTSGKRVLKKSGLAAGKGVLESSDPGEL
                     LSFGRSVLAGDVLLAEEFLTGRELSIFAVCDGTRRVVLPACADHKKPHPGNLGPNTGG
                     MGALCPVPFAGPDLLARIEAEVVEPTFRGIAREGLAYPGVLFLGIMAAPDGPKLLEYN
                     VRFGDPETQSLLAVLGGNLGDLLASAASGRLDPGAGAAAAAAPDTWACGFVAAAPGYP
                     GSYPKGIPVEALPEDNPGRSLLFHASTLRDASGRLVTGGGRCFTAVGLGSTPAEAREN
                     GLAAARGIRFEGAWFREDIGSTV"
     gene            complement(11220..11290)
                     /locus_tag="P5313_09320"
     tRNA            complement(11220..11290)
                     /locus_tag="P5313_09320"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11256..11258),aa:Gly,seq:ccc)
     gene            complement(11617..12495)
                     /locus_tag="P5313_09325"
     CDS             complement(11617..12495)
                     /locus_tag="P5313_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRY80605.1"
                     /translation="MKTSAARKNIRTTELTSDENILSLYLKEINRIPLLTREQEDTYA
                     RAAARGEQAAKDMLVKSNLRFVVNIAKKYQNQGLPLSDLISEGNIGLINAIERYDVDK
                     GYHFISYAVWWIRQAILKAVCEKSRMIRLPLNRANELVQIEKARKFVTGGMSEDGEIR
                     EIARMLDMDADHVADLVNVSRDLISLETPVFDERDSSVVGDFVENDAYVSPENTAIEN
                     NLREDINSVLRTLTEKEREVIQYRFGLNGRRAMSLKEIGERFSLTKERIRQIEKNALK
                     RLSQSNRSEILESYVA"
     gene            complement(12758..13420)
                     /locus_tag="P5313_09330"
     CDS             complement(12758..13420)
                     /locus_tag="P5313_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80606.1"
                     /translation="MRGNEHDKLERNYRAGMRSLARHDAQAALRHLRAAVDACPPGRH
                     AALARYLYWLSIPLLRLGKSELAVKSLVSAQKLEPRGAARRLYRHMVNGYGMVSTGCA
                     DKDDFRAFFSIQLRRYLGSRPGGRFRTEAERDAVARIIADSWLRLTGAGALAEKSCSD
                     KLELFQAFEIPFPFRFLDKAKVLPGNFRRGTLQKSDARCSCGSGLPFRQCCGRTQSAF
                     GS"
     gene            complement(13503..>14390)
                     /locus_tag="P5313_09335"
     CDS             complement(13503..>14390)
                     /locus_tag="P5313_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HRY80607.1"
                     /translation="EFWRGDRHEVRHFCTRITGSSDLYLRDGRKPFHSINYVTSHDGF
                     TLRDLVTYERKRNRENGEGESDGHDANFSCGYGADGPTEDTGILAVRERQARNLLATL
                     LLSIGTPMLLGGDEFLRTQGGNNNAYCQDNPVSWVDWTLADENRGLSRFVRELIALRL
                     RHPAFRRPEFFTGKDANYNAIPDISWYDPSGKAPDWARIDRMIAARIDGSRADIVADR
                     DDNDFFLMFNASDAEVRFRICPPPPDTRWHLSVDTARPSPQDIREPGTEPVLSPQEEY
                     LLAPRSLVLLLARDPGAGI"
BASE COUNT         2315 a         4826 c         4844 g         2405 t
ORIGIN      
        1 accgggttga ggtcctcgcg ctggacattc tcgatcaggg cgatctcgag cttcttctcg
       61 ggggtgaagg accggaccac cacggggact tcccggagcc cggccttttc ggcggccttg
      121 tagcgccgtt cacccgccac gatcaggtag gtcccgtccc cggaatcctc cacgatcacg
      181 ggctggatga tcccgtgcag gcggatcgag gcggccagct cctccaggga ggcttcgtcg
      241 aaggtcttcc ggggctggag ggggttgggc acgagccggc ccaggggcac gctccgcagt
      301 ccctcgggga cggggggccg gtgctcgccc tcgggacctt ccagggcctc caggcggacc
      361 tcgacctcgc tttccgggat cagggccccc agtcccttgc ccaggccgaa cttagccacg
      421 ggacaggatc tcccccgcca ggcgctcgta gctcttggcg cccacgcagt cgggatcgta
      481 gaggcagacc gggaccccgt gggagggggc ctccgagagc cgcacgttcc ggggaatgat
      541 ggtccggaag acccggtcct tgaagtactg ggtgacctgc tggaccacct gctgggccag
      601 cttggtgcgg gaatcgtaca tggtgaagag gatccccccg atccgcaggc ggggattcat
      661 gcttttctgt acgaggctca cggtctggag gatcagggtc aggccctcca gggcgaaata
      721 ctcgcactgg aggggcacca atacctcgtc ggcggcattg aggccgttca gggtcaggat
      781 acccaaggag ggggggcagt cgatcaggac gaaatcgtac cggtccagga cgggccgcag
      841 ggccttcttg aggtagtcgt tccggtcctc cttgtccacc agctccacgg tcgccccgga
      901 gagttcgatg gaggacgggg cgaggtccag gccctcgacc ttggtcggca cgatgacgtc
      961 cgaaagggcc aaggccccgg acagcacctc gtagatgccc cgggaaccgg tcttcccgcc
     1021 caccccgctg gtgaggttgg cctggggatc gaaatccacc aggaggaccc gcttcccggc
     1081 cttggccagg tacgctccga ggttgatggc ggaggtggtc tttccgaccc ctcccttctg
     1141 gttgacgaaa acgacggttc tggccatcgg aacatccttc tcccgagccg ccccgaggcg
     1201 cggcgcggca ttctaccata tccgggccgc cccgcgctac cgaagcccgg gcggcttgac
     1261 cggccttccg acatatcagt actgtaccta catagggcgc ggctcccgac atggttacgg
     1321 aagttccggt ttttgggcgc accgccgatt ccgctccgcc gggagcggga attcccgcga
     1381 tcgaggtaca ccatgctcaa ggaaaaggtc cagaaagcca tacaggacgt gcgcccctcc
     1441 ctccaggccg acgggggcga catcgagctc atctccgttt ccgacgacgg cctggtgaag
     1501 gtccgcctca agggtgcctg cgggtcctgc cccatggcca ccatgaccct gaagaacggg
     1561 gtggagaact acctgaagaa ggttatcccg gaggtgaagg aagtcgtgca agggtaaaaa
     1621 accgcctacg gcggcttttt acccttgcag cgacgaagcg cgcccggaag gcgcgcttcg
     1681 tcgatttacc gatctgcttt tttgacggcg gttttcagcc ttgcaacgac gaagcccgcc
     1741 tgccggcggg cttcgtcgaa ttctacagga aacctttatt ggcagccggt tttcgcgcag
     1801 cgaaaaccga tcgctgagcc gcgctcgggg ctgcgccccg aggcaacgaa gcccgccgga
     1861 aggcgggctt cgtcgaattc tacaggaagc ctttattggc agccggtttt cgcgcagcga
     1921 aaaccgatcg ctgagccgct atgcggctca gcggactcca tcgtcacatg cgtcatccgg
     1981 ctggttgaac cgggcggttt cggcggaatg ggccttgccg cagaggtcca tgggaatttg
     2041 ctgcatctcg tggccgcagc agtcgggagc ggagttcgtg tccttcacga tcttctcttt
     2101 cccgcagtcc ttgcatttat acgccttgtc cgccatatgc cctcctatcc aacctggggt
     2161 tcgtttccta cgttcaatgt actgcctcgg gggcggttcc gtaaacatcg accgccgaag
     2221 gcggttcaac gattaaggcg tgccagctcc gagatcctgt ccgccatccg ctccaggggc
     2281 acctgctccg tgacatgccc cagttcgaag gccaccttgg gcatgccgta gaccacgctg
     2341 gaagcttcgt cctggcccag ggtgatgccg ccctcccgga acagggcgcc cagctcccgg
     2401 gccccgtccc ggcccatgcc ggtcatgatc actcccaggg cccggttccc gtattcccgc
     2461 gccacgctgg caaagaggac atccgcgctg gggcggtgcc cgttttccgg gggggcgtcg
     2521 gaaaggcgag ccacggcggc caggctcttc ttctccacca gcaggtgctt gtcccccggg
     2581 gcgatcagga cccggcccgg cttcaggaga tccccgtccg cggcctcctt cacgtccagg
     2641 gggcagatcc ggtccagact gcgggcgaac tcctccgtga agcccggggg catgtgctgg
     2701 accaccacga caggcgggac cagccggggg tcgatccggg cgaagacctg gcgcagggcg
     2761 ttcggcccac cggtggagat gccgatggcc accaggtcga cgggtccggg ggaccgggcg
     2821 ggcacgggct tttccgccgg cttcggggaa cggcgggcag cgggaggcgg aggagccgag
     2881 gtgccgggct ccttctccag catcagctcc ggggattcga aggggtacgc ggccggggtc
     2941 ttgccccggc tcttctggta ctgggtaccg taggccagga ggagccgggc aagctctccc
     3001 gccacggtgt ggatgtccga ggagaccgag ccggagggct tggttatgaa gtccgcggct
     3061 cccagggcca gggcctccat ggtgacctcg gcgcccttct tcgccaccgc ggagagtacg
     3121 accacgggta tctcgatccc caggcgacgg cgctccttca ggaactcgat gccgttcatc
     3181 tcgggcatct cgatgtccag gacgatcacg tcgggattgc agcgcgggat cttctgcagg
     3241 gcgaacaggc cgttcatggc cctgtccgcc accttgaggc ccggggtcgc ctcgatcatc
     3301 ttggagacga gattgcgcat caacgcggag tcgtccacga ccagcacgtt cacgggatcc
     3361 ggcactctgt tcctccatgc atggcgtacg gctcgccggg cgggcccggc cgggtcgggg
     3421 aaggatccgt catggcttca cgtccttctt gtagaagcag gcccattcgg tcttgacgaa
     3481 ctcgaaccgg gtattcatcc cgaaaaggga ctcgctgtgg ccgatgaaca ggaaggactt
     3541 gggtcccatg gtctcgtaga agcgctccac cgcggccttt tgggcgacct cgtcgaagta
     3601 gatgaggacg ttgcggcaga agacgacatc catgttccgg agtccggaat cgttcttgag
     3661 gttgtggtag tcgaagcgga cgagcttttt gacgtcctcc cggatctgcc atccgttggg
     3721 cttccgctcg aagtacttgg gcaggtacgc ttcggggatc ccgacgatcc gcgagtccgg
     3781 atagaaaccc tccttgccga ccatgaggga cttgaggctt atgtcgctgg cgacgatttc
     3841 gcaggtccag ccggccggaa ggatctcccg gagcaggagg gcgatggtgt agggttcctc
     3901 cccggtggag cagccggcgc tccagatgcg gagcttgcgg tccggagagg tcccccggat
     3961 ccggatgagt tccggaacca cgaacttctc cagggcgtcg aagtggggct ggttccggaa
     4021 aaaccgggtc aggttcgtgg tcaccgagtc cagcaggatc ttgagctcct cccggtcccg
     4081 ggttatcagg ttgaagtagt cccggaggct gtcggtcatc ttctcccgca ggcgttcccg
     4141 caggcggctt tccaggatgg atcggttggt ggcggagaaa tggataccgc tctcgtcgta
     4201 gatcagcttc cggtacagct cgaactcggc gtcgggtagg aaatcggaca taggaacgcc
     4261 cttccgctcg ccgctatacc gcggcggctt tcgatggttc gacgccgacg aatcgctcct
     4321 cggctctcgc gtcccggaca cgagacgctc cctaccaggg gaatcttcgc cgggcatgtt
     4381 caaagctggt ttccgaggcg gttttatact gcgggagtcc aaatatcagg atcccgtgct
     4441 gaaaatctag tgccccgttt ccgggggtgt caatagtggc ttccggtccg cccggaaatc
     4501 attcctgaaa tttcggaaaa cccgggataa acccatcgca gcccgcgaat tcccggatcc
     4561 ccgggatata gtatggagta tggcaaccga ggatgtcgta ctgtccgtgc ggggcgtcgc
     4621 cgccgggttc ggggacgagg ctcccctggt cgtgctccag gacgagcccc gctcgtcctc
     4681 cctaaggatc cccgtgggcc cctacgaggc cagttccatc atcctggagg tggagggcat
     4741 cgcgccctac cggcccctgg cacacgacct cctggccacc ttcttccggg accacggttt
     4801 cctcctggag tccgcccgca tctacgacgc gattcccgac ggcgacggcg gggagcggtt
     4861 cctgtccagg atccggtacc gcaagggtct ccgcaagtgg gagaaggagg tccggccctc
     4921 ggacgccatc gccctggccc tgcgcctggg ggcgcccatc ctggcgcccc gggagttcct
     4981 ggaccgccgg gaagcccgcc gggccgccgc ggccggggac acccgcctac ccctctacct
     5041 gcaggtcccc cgatccgagg cagccggttc ccgcagcgat cggtaggacg cttcggccct
     5101 tgccgatcgt cccgggggaa cctagaatac caggtatgaa tattttctcg tccctcgtgg
     5161 agttcctgcg cggcatcctc ggccgcgggg gacccgacgc ggcaaaaaaa gcggaattaa
     5221 gaaagatcta cgcctacctt tcccagatcc gtccacccta ctacaagccc aaggggaacc
     5281 tggtcctgcc gggctttgcc cagggggtat acaatttccg ggcgatcctc cggcccctgg
     5341 tggacatggc ccgcaagacc atctcccatt cggacatccg gatctaccag cgcttcttcg
     5401 acctcctcgt cgacgcccgc ctgcccgagg agacccggca gaggaaggcc tctttcgcct
     5461 acgaggggat gaaggcccgc atcgcgaacg ccgtgaagga ggacgaggag ttcgagatca
     5521 tcggccggga gttccaggat ttcctgcacc aggtggattc cctctcgatc ggcgacctcg
     5581 accgcgagct cgcggaagtc gagcagttct ccgacatctg ccgcttcgac tgggaccgca
     5641 tcatcggcct cttcgacccg ggggccaaca tggacaaccc cggctaccgg ccggacttcc
     5701 ggcccgcgga aggcgagaag gtcctgcccg agctcgtgga cctctactac atcctggggg
     5761 atttcgcctt cgaggagggg ctggcccaga acctcctcat catcctggag cgccaggcgc
     5821 cccagacccc ggtctccacc cagaggcaga aactggacaa gatcttcgcg gccctcaaca
     5881 agggcctggc cctccgcctc caccgggacg tcctcctggc cctgatccgg gccctgaagt
     5941 cggaccccgt ctacgatccg cccctggtgc gggagacaaa cgactacgtg gagcgctacc
     6001 ggcgccgcat ggtcacccag tacgagaagg accgggagcg gctccagcgg gagcagcacg
     6061 agaacgccat ctccacggac atcgacgccc tgttcggcgg cacggaggtc gcccccgtgg
     6121 acggctacga cgaggaggag gacgcctacc tccgccggga aagccccaac gggttcaccc
     6181 acatcaagcc cctgcggatc ctcaagacct tcgtccacaa caccttcgag aaggagatca
     6241 aggagccggt caagcgcatc ctggtggagg ggtacttcga caacaagggc ttccagaaca
     6301 accttgccaa catcatctac cagtgcgaga ggtccgcggg ccgcatcgac gaattcgagg
     6361 cctccctgcg cggcaacggc cggatcagca tcgtggccat gaagcgctac gtggaggaga
     6421 tgcgccgggg caaggacgtg tcccagttcc tgaaccgcct ggtggacgcc atcaaccagc
     6481 gggcccggga gatctgcgag gacgaaaccc ggctcctgca gatgctcgag gagtccatgg
     6541 gggacctgct gaccgactac cgccgcccca gcccggacct ggtcaccaac atccgaaccc
     6601 tggggggagc ccgcaacaag gagatcctgg gccaggtcgc ggagggccgg cgccacctgg
     6661 acaccctcct ccggatcatg cggaacttca cctacatccg cgcgccggga acgcctcccg
     6721 ggacgcccgg ggtcgcggtg gagcccccgg gcctggcgga ggcggaaccg gccccctagc
     6781 ccctcgcctt gacgggccgg gacggggcgt gctagaaacg cccgtggaat cccacgacga
     6841 ttcttcccta ggacactact gcggagtggc cggaatccgg tccccctccc ccctcgacgt
     6901 accctccgcc ctcttctaca tgctcttcgc cctccagcac cgcggccagg agagcgccgg
     6961 aatcgtctgg gccgaggggg gcgcccaccg ggcggaccgg cggctgggca tggtctccgc
     7021 cgccgcggac gcctggcttt ccggccgggc cgcctcgacg ggaggcatcg ggcacgtccg
     7081 gtactccacc gcggggggcg gcgggatccg gaacgcccag cccctggcgg tggaatgctc
     7141 caaggggagc gtggccctgg cccacaacgg caacatctcc aactccgcgg ccctccgggc
     7201 ggccctgacc cgggaggggg ccatcttcca gtcctcctcg gattccgagc tcatcctgca
     7261 cctgatcagc cggagccggg ccgaggggat cgaggccatc ctgcgggagg ccctggcccc
     7321 cctggagggc gcctacagcc tctgcatgct ctggggggaa accctggtgg cggtccggga
     7381 cccctggggc ttccgccccc tgcacctggc ggaaaaggac ggggtccggt acgtggccag
     7441 cgagacctgc gccctccacg ccctgggtcc ctcgatctcc cagcgggagg tcctgcccgg
     7501 ggaggccgtg atcgtggacg atcacgggga gcggtccttc ttcctgcctg cggccccccg
     7561 gcgggcccgc tgcgtcttcg aactcctcta cttcgcccgg ccggactccc gggtcttcgg
     7621 ggagtccgtg cacgccgccc gggaacgctt cggggaggcc ctggcggagg cggatgacgt
     7681 ggaagcggac ctggtggtgc ccgtcccgga ctcggggaac ctggccgccc tgggctacgc
     7741 ccggcgggcg gggatcccct tcgagttcgg gatcacccgg aaccactacg cgggccgatc
     7801 cttcatcctg cccacccggg cggagcggga gttcgcggtg cgcatgaagc tacacccggt
     7861 gccggagatc gtggggggca agcgggtggt cctggtggac gactccctgg tccggggcac
     7921 cacgagccgc ctgatcgtgg gactgctcaa ggaggccggg gcccgggagg tccacctccg
     7981 cctctccgcc ccggagctca cgggcccctg cggctacggc atcgacatcc cggaccggtc
     8041 cgagctcatc tccaaccgga tgagccccgc ggagatcgcc cggtacgtgg gggccgacac
     8101 ggtccgcttc ctgcccccgg acctcctgcg gagccgcctg gccgaaccct ccgaatactg
     8161 ctacgcctgc ttcgacggcg actacccctg ccccatccgg aacggggagg gaaaggaaac
     8221 cccatgcccg tagacagcta caagaaggcc ggagtggaca tcgccgccgg cgacgccttc
     8281 gccgacttca tccgggacct gccctccaag gcggtgtcca aggccatcgg aggcttcgcc
     8341 ggcggggtgc ccctggacct gagcggctgg aaggagccca tgctcctgtc caccacggac
     8401 ggagtgggta ccaagctcct ggtggccaag aagttgggac gctgggacag catcggcatc
     8461 gacctggtgg ccatgagcgt gaacgacctg gcggtctgcg gggccgaccc gatctccttc
     8521 ctggactaca tcgcctgcgg gcggatccgg gaggaggtcc tccgggacgt gatccggggg
     8581 gtcgtggcgg gctgcgaaca ggcggactgc gttttggcgg gaggcgagac cgcggagatg
     8641 cccgacgtct acggcccgga cgacctggac ctggcgggct tctgcgtcgg agtcgtggag
     8701 aagtccggga tgctgcccct cctctcccgc atcgaggcgg gggacgccgt cctgggcctg
     8761 ccctcctcgg gggtccactc caacggttac tccctggccc gtaagtgcgt tcccgaggac
     8821 gaccggaccg cctgggagga actcctgacc cccacccgga tctacgtccg ggagcttcga
     8881 ctgctccggg agacgggaaa gctcctggcc gcggcccaca tcaccgggtc cggcctggag
     8941 gccaacttcg agcgggtgat tccgaaaagc ctaaaagccc ggttcacctg ggactggccc
     9001 cggccgggca tcttcgaccg gatccaaagg ggcgggaacg tccccgagga ggagatgcgc
     9061 agggtcttca acctgggggt gggtatcgcc ttcgtggtga agcccgcgga tgccgactcg
     9121 accctggccc tctgccggga ccgaggcatc ccctgcttcc ccctgggcga gctcgtccgt
     9181 gcctaatctg gccgtcctgg cctccggctc gggctccaac ttcgaggccc tggcccgggc
     9241 cctggagccc acggagcacg agtgcgtcct cctggtctgc gaccgggcgg gcgccggggc
     9301 cttcgagcgg gcggccctcc tgggcgtgcc cgcccggcac gtgtcctaca cgggtcgccc
     9361 ccgcgccgag gcggaggccg agatcgaggc cctcctgtcg gaggcgggct cggacctggt
     9421 ggccctggcg ggcttcctcc gcctcctgtc cccggacttc gtccggcgcc gggccgggcg
     9481 gatcctgaac gtccaccccg ccctcctgcc cgcctacccg ggcctggacg ccctccggcg
     9541 cgcctgggac gccggggagg aggtcttcgg cgccaccatc cacctcgtgg acgagggcat
     9601 ggacaccggc cccgtcctcc gccagggaaa cctcaagcgg cgtcccggag agagcttcga
     9661 ggacctggag gcccgggtcc acgccctgga gcacgagctc tatcgccgga ccgtgctcga
     9721 gctgctggac gggatcgccc gcgaaggctc cccggggggc cggcggtgaa cgtcctggtg
     9781 atcgggtccg gcgcccggga gcacgccctg gcccggcgcc tcgggaccga agggaaccgc
     9841 gtctcctgcg cccccggcaa cgcgggcacc gcggggatcg ccgacaacct gccgctcaac
     9901 ccggacgacg cggagacggt actccgggcc tgccgggagc tcggggccga cctcgtggtc
     9961 atcggccccg aggatcccct ggccgcgggc ctggcggacc ggcttcgcga cgccgggtat
    10021 cccgtcttcg gccccggcgc ccgatccgcc cgcctggaat ccagcaagga cttcgcccgg
    10081 gccttcgcgg aacgccacgg ggtaccccgg gcccgcaccg aagtccttcg caccgaatcg
    10141 gacttgcggg actacatcgg ccggacctcc gggaaacggg tcctcaagaa gagcggtctg
    10201 gccgcgggca agggggtcct ggagtcctcg gatcccggtg agctcctttc cttcggccgc
    10261 tcggtcctgg ccggggacgt gctgttggcg gaggagttcc tgacgggacg ggaactctcg
    10321 atcttcgcgg tctgcgacgg gacccggcgc gtcgtccttc cggcctgcgc ggatcataaa
    10381 aaaccccatc ccgggaacct tggcccgaac accgggggca tgggagccct ctgccccgtg
    10441 cccttcgccg ggccggacct gctggcccgg atcgaggccg aggtcgtcga gccgaccttc
    10501 cggggcatcg cccgggaggg ccttgcctac cccggggtcc tcttcctggg gatcatggcc
    10561 gccccggacg gcccgaagct cctggagtac aacgtccgct tcggggaccc cgagacccag
    10621 tccctcctgg cggtcctggg ggggaatctc ggggacctcc tggcttcggc ggcctcgggc
    10681 cgcctggacc cgggggccgg ggcggcggcc gcggcggctc cggacacctg ggcctgcggc
    10741 ttcgtggccg ccgccccggg ctacccggga tcctacccca agggcatccc cgtggaggca
    10801 ctgcccgagg ataaccccgg ccgaagcctc ctgttccacg cctccaccct gcgggacgcc
    10861 tccggccgcc tggtcacggg gggcggccgc tgtttcacgg ccgtggggct ggggtccacc
    10921 ccggcggaag cccgggagaa cggcctggcc gcggcgcggg gcatccgttt cgagggggcc
    10981 tggttccgtg aggatatagg ttcgaccgta taggagccac tcaggactca acattcagga
    11041 ctcaccacag aggcgcgcga tgccggctta gccgtcatcg cggcagcgag cttgcgctcg
    11101 ccgaggtttc cgcaaacctg ttccgagtcc tgtcgcctga cacctgtcga caaaaaaaag
    11161 ccgagcctga aagctcggtt tggggaggga acgcggattg agcgctccag cgccgatcga
    11221 gcgaccgatg ggagtcgaac ccacttctcg agcttgggaa gctcgggtaa taccggtata
    11281 cgacggtcgc tcgctcggcg ctgagccgtc cccgcgggac ggccgctcaa accgcgctcc
    11341 ctgaagggac ggccgttcgg ccgcctcggg acgcgcgatg tgcgcaggat catgatacga
    11401 aaaaccacgc cggttgtcaa cctgaagtcc gctgcgggcc gccgactccg atagaaaagg
    11461 ggctgtccaa aaagctcgga tagcttttta gacagctggt attagtgtag cagtttctaa
    11521 cgttctcaag agaacgttag aaactgcaag gggtgtccaa taagcaacca acttattgga
    11581 caccccggat ctatataatt ccggagtctc cgtgcctcag gcgacgtagc tctccaggat
    11641 ctccgagcgg ttcgactggg acaggcgctt cagggcattc ttctcgatct gccggatccg
    11701 ctccttggtc agggagaagc gctccccgat ctccttgagg gacatggccc tgcggccgtt
    11761 cagcccgaag cggtactgga tgacctcgcg ttccttctcc gtcagggtcc ggagcacgga
    11821 gttgatgtcc tcccggaggt tgttctcgat ggccgtgttc tccggggata cgtaggcgtc
    11881 gttctccacg aagtccccca ccaccgagga atcccgctcg tcgaagaccg gggtctccag
    11941 ggagatgagg tcccgggaca cgttgaccag gtccgccacg tggtccgcgt ccatgtccag
    12001 catccgggcg atctcccgga tctccccgtc ctcgctcatc cctccggtga cgaacttgcg
    12061 ggccttctcg atctggacca gctcgttggc ccggttgagg ggcagacgga tcatgcggct
    12121 cttctcgcag accgccttga ggatggcctg gcggatccac cataccgcgt aggagatgaa
    12181 gtggtagccc ttgtccacgt cgtagcgctc gatggcgttg atgaggccga tgttcccctc
    12241 gctgatgagg tccgataggg gcaggccctg gttctggtac ttcttggcga tgttcacgac
    12301 gaagcggagg ttcgacttga ccagcatgtc cttggcggcc tgctcgcccc gggccgcggc
    12361 gcgggcgtag gtgtcttcct gctcccgggt caggaggggg atgcggttga tttccttgag
    12421 gtagagggag agtatattct cgtcgctggt gagctcggtg gtgcggatgt tcttacgggc
    12481 ggcagaagtc ttcatgattc ctcccgccgt ctatatagca gggtccgtgc caagtcgagc
    12541 gacagacgtc gggaatcagg agacgggtag gaacggccgg cggaaaatcg aatccatttg
    12601 catgtatatt aaataattac aagggctcct aataatcgcc gcggcgggcc cggaggcctg
    12661 cgccgcgtcc atgcccaggc ctcggttttg caagtccatg tttcctttcc acacggtctg
    12721 cgggaagggg cttaaaaccg tatcaaaaag atccagatca cgagccgaag gcggattggg
    12781 tgcgcccgca gcactggcga aagggcaggc ccgagccgca ggagcaccgg gcgtccgatt
    12841 tctggagggt tcccctgcgg aaattgcccg gcaggacctt ggccttgtcc aggaaccgga
    12901 aggggaaggg gatctcgaag gcctggaaga gctccagctt gtcggaacag gacttctcgg
    12961 ccaaggcgcc cgcccccgtg aggcgcagcc acgagtccgc tatgatccgg gccaccgcgt
    13021 cccgctccgc ctcggtgcgg aagcggccgc ccggccggga tcccaggtaa cggcggagct
    13081 ggatgctgaa gaaggcccgg aagtcgtcct tgtccgcgca gcccgtggat accatgccgt
    13141 agccgttgac catgtgccgg tagagccggc gcgcggctcc ccggggttcc agcttctggg
    13201 ccgatacgag actcttcacg gccagctcgg acttccccag ccgaaggagg ggaatggaca
    13261 accagtacag gtatcgggcc agggccgcat ggcggccggg cgggcaggcg tccacggcgg
    13321 cgcgtaggtg cctcagggcg gcctgcgcgt cgtgccgggc cagggatcgc atccccgccc
    13381 ggtagttccg ttccagcttg tcgtgctcgt tcccgcgcat gaggataagg tacgaatacg
    13441 ggagcgccga atcaaggcgg cccgccgcgg ggcctgcatc gactacggcc ggcgcgggga
    13501 cgtcagatac ccgccccggg atcccgggcc agcaggagga ccaggctgcg gggagccagt
    13561 aggtactcct cctgggggga caggacgggc tcggtcccgg gctcccggat gtcctggggg
    13621 gagggccggg cggtgtccac cgagaggtgc cagcgggtgt ccggcggagg aggacagatc
    13681 cggaaccgca cctcggcatc ggatgcgttg aacatcagga agaagtcgtt gtcgtcccgg
    13741 tccgccacga tgtccgcccg ggacccgtcg atccgggccg cgatcatccg gtcgatccgg
    13801 gcccaatccg gggccttgcc ggaaggatcg taccaggaga tgtccgggat ggcgttgtag
    13861 ttggcgtcct tgccggtgaa gaactccggg cgccggaagg ccgggtgccg caggcgcagg
    13921 gcgatgagct cccggacgaa gcgggaaagg ccccggttct cgtccgccag ggtccagtcg
    13981 acccaggaca cggggttgtc ctggcagtag gcgttgttgt tccccccctg ggttcggagg
    14041 aactcgtccc cccccaggag catgggggtg ccgatggaca gaaggagggt ggccagaagg
    14101 ttccgggcct ggcgctcccg cacggccagg atgccggtgt cctcggtcgg cccgtccgcc
    14161 ccgtagccgc aggagaagtt ggcgtcgtgg ccgtcggact cgccctcccc gttctcccgg
    14221 ttccgtttcc gctcgtaggt gaccaggtcc cggagggtga agccgtcgtg gctggtgacg
    14281 tagttgatcg agtgaaaggg tttgcggccg tcccggaggt agaggtccga ggagcccgtg
    14341 atccgggtgc agaagtgccg gacctcgtgt cggtcgccgc gccagaactc
//
LOCUS       DAOTEI010000084        14230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2952_length_14230_cov_5.030335, whole genome shotgun sequence.
ACCESSION   DAOTEI010000084 DAOTEI010000000
VERSION     DAOTEI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14230
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2952_length_14230_cov_5.030335"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1476)
                     /locus_tag="P5313_09340"
     CDS             complement(67..1476)
                     /locus_tag="P5313_09340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY80608.1"
                     /translation="MITFYSEGAAGEVTGSKHVLDIDGRRFLVDCGAFQGKRETAEKK
                     NKALVPDPAGLEAVFLTHAHYDHSGMLPLLSKRGFRGNVFATPATRDLAGIIMMDSAR
                     IQARDAEYLAKQAAKRGEPFTWKPLYDEQDAVQAVGQFVTVSYNRPLSAGDGVRIEFF
                     DAGHILGSSMIVAEAKDSGGRSATIAFTGDLGRKGKPIIRDPDRIPPVEWMVLEATYG
                     DRLHEAQDEALEKLARVVRETAAKGGKVVIPAFAIERTQELVYYLHLLSDRKAIPEIP
                     IWVDSPMAINATTIFQVHPECYDRETHDAFLKHHKNPFGFNHLRFSSSVEESKALNDL
                     KGPAIIISADGMCEAGRIQHHLLHTVEDPANTVLIVGYMAADTLGRRIKERVPEVRIH
                     GNILKLRARVEEINAFSAHADYKETWDWMSGLDLSRLKGVFLVHGETESLAAMSSFLG
                     GKGIRDVRIVKYGDRHDLV"
     gene            complement(1519..2421)
                     /locus_tag="P5313_09345"
     CDS             complement(1519..2421)
                     /locus_tag="P5313_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY80609.1"
                     /translation="MQRIGELAAFGTAICWTVSAVFFEKASRRIGTVAVNLYKLVGAI
                     LLLSGTAWILRGLPFPTDAPGRAWLWLSASGLVGFVIADIFLFNAYVMIGSRTTMLFL
                     ALSPPTTAFFSFLAIGELMPPRGLAGMALVCAGIAAAVLGRPRDPRAELVQDRDKRRK
                     GYLFAFLASVGQSAGMILTKLGSAGYDTVAATQIRVLAGMAGFALVALVSGKGRKVFV
                     EAPGDRAGMRNTAAGAVFGPFLGVTLSVFALNHTQAGTVSTLIGLAPVLIIPPSVLFL
                     KQKVSLWEILGACVAVGGTVVFFL"
     gene            2563..3384
                     /locus_tag="P5313_09350"
     CDS             2563..3384
                     /locus_tag="P5313_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HRY80610.1"
                     /translation="MAVICIARELASLGEETAHELADLTGYRYLDKAYVEKKLAEYGI
                     AATEIQKFDEKKPGFWASLSRGRDDYLHFLKTVLYEEAQIGDCILVGRGGMGIFAGVP
                     GVIAVRLTSPLPVRIERIKKFYNCDERHAEQLARQSDNDRDGFHRYFFNLDWRDPVNY
                     DMVLNTGLEHPATVAQIINQYRLLTITKDLESKCGQRLRELSLGQKVITSVLFDKGIS
                     IHFLEADVKGDLVTLHGVANTQQTIDAAVAAAHEIPGVGHVESAIQVVQEYTVMP"
     gene            3650..4372
                     /gene="rlmB"
                     /locus_tag="P5313_09355"
     CDS             3650..4372
                     /gene="rlmB"
                     /locus_tag="P5313_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRY80611.1"
                     /translation="MQLLTGFHAIEEALKRGQVRGYLRVSGTGPRIKRILELADKIGL
                     RVEKAKSGDLDAAAPDNRGIVLTLEGDSRPANDADLDEFLEKLSKPEALVLLLDHVTD
                     PHNYGAILRSADVLGADLVVVPERRSAHDTDTVARASAGAVAWVPVAVVPNLVRALTA
                     LQENGFWGYAADMAGIEIGQAKLPERTAIVLGSEGYGVSRLLKETCDGSVSIPQRGNV
                     ESLNVSVAAGIVMWEYRRRYAG"
     gene            complement(4373..5953)
                     /gene="pyrB"
                     /locus_tag="P5313_09360"
     CDS             complement(4373..5953)
                     /gene="pyrB"
                     /locus_tag="P5313_09360"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRY80612.1"
                     /translation="MERTGPFRGRTIAVVDDLSGDEQRYLYSLARDIKDTIRSGRDPS
                     RFRISDPDYQTYLIFLENSTRTRESFRNAAEFHGCRVNMFDAATSSFAKNESLADAAK
                     MLVGYAAESCFVIRSGLEGVCRWLDSSVGTYCESAGFPKPAFLNAGDGKHEHPTQEFL
                     DEFTFLEHRGGDDSRIHLALVGDLWHGRTVHSKAEGLRIFKEVSVDLVAPELLALPPA
                     YVERMKASGFEVRAYESLDEYLASGRVAPIWYYTRLQLERMGERVLDRAAELRRTVTF
                     RPDMMSRLPEGTRFYHPLPRDRTAPEIPVFLDELPLNGWDAQSANGYFVRIALLGLVS
                     GALGGDFRGLPKEPLRFEEDFVTEAKVTPRVKPDYKVGIKPVESGIVIDHIAAGRPIR
                     EIWDTVDKVRRILGLDVRSSHGVYHSNAGPEVYKGILSLPDILAFDRRDLKKLGAIVP
                     GCTLNLIKDSRVLKKYRLAMPPRIYNFDQISCKNEACVSHPSHREGAPARFRRTGERI
                     FTCQYCDREHEFGEIWDL"
     gene            complement(6134..7693)
                     /locus_tag="P5313_09365"
     CDS             complement(6134..7693)
                     /locus_tag="P5313_09365"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HRY80613.1"
                     /translation="MKKYLALPALLAFLTAGAILSAQTVAEDELRSVGGRTIEFINYV
                     GPHARIDSLDEIRGLGAALGRAVRAGATRAGSETRYAVIRVRDPQAPSGLDADIIILG
                     RDAGVDHIRNLRHILGAYLEEAYGYSRSDGFLLATFLTVYNAVYRGDLAYFGKAYKPA
                     VLTHLTAENAGLSVRWDEWAGRSRIVLPITARAGTPGRVDTGPITDKSVVESLKEESG
                     TAALPERREMVDLKEREVEQDKARLEEDKARIAEQEKAAAEETARAEAARAELERDRA
                     SAQGPEQEAELTRREAEVAAQEKAAAEKEAAVAAAKEETTKEEAAVAAKEAEIAEDRK
                     AVAEDQKAEIAKEVAAKAEAAPRGSVLLDLVDPNRPYARLALVDLAGGKYLKTGEINT
                     LRADSLLDIGGAFVAVAGRAEGSGAVRLVLLDLETLSAKVTGTVDVHPETGVWKEGAG
                     ILAVANDGGKWVLGLFDPATLELKMKSEAAVSPFTFLRSVPEGLVVAGAGGGLILLNK
                     ADLKTVKEIRR"
     gene            complement(7706..9454)
                     /locus_tag="P5313_09370"
     CDS             complement(7706..9454)
                     /locus_tag="P5313_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY80614.1"
                     /translation="MSAFAGTEPRRVRGRTRYPIALFAAAAILAVPSNAQSPVQDILA
                     FRFTLAGAAAAGPVAEAGRVWIVSEDRTLYILDDSGRAVGRRAWRDGSPAWIAPDPFG
                     RALLPAADGSLVMVNRAGLEVWRVRPGVPVVRAPAFGADGRFFAAAASRLLAWSASGR
                     LLWSVEHAAPIAAGPSVRFTPSGKTRVTLALADGSIREYAEDGTLLSSVPDGTSSSVP
                     VSEPPSALCGIGEGFVLAYRNGAVSVRDGAGQETYRVRLAAPVVAAAGDRAEAWLLLA
                     GGSVVRVREGGTVSDTTSTGILDAQDIRVFPERVLVLGPRGAASLAKDGQVYRALSLR
                     HAPRMPAVTSSGIVLSAGEDWILYAYRFEGPLGEPARPAPGYVDFAAARARANEEIFW
                     NPGFGDDAALRELRRIEKSLGSASIGAEEGDALLLCSAIALGYGAREASVYGGAAPRG
                     PRPSGALPRALACDILGMLGSPRAVPVLTEAYLRDPEPAVRASAARAIGIIGLDLTGE
                     ALRAFQGAADREFFLDEPGALATIDAVEGIYRALGELSDPGGVRAVLRLADKPYGSAV
                     RSRALAALRRLSEPAR"
     gene            complement(9432..10628)
                     /locus_tag="P5313_09375"
     CDS             complement(9432..10628)
                     /locus_tag="P5313_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY80615.1"
                     /translation="MSDRDIVELRIEKLAVGGDGLAFPEGKAVFVPGTVPGETVRAAF
                     PDGVRASGDWAAAELREVLEASTDRIDPECALYGICGGCSLQHMAYSRQVREKAEILR
                     DCLSRIGGLSVRDIDEVPSEPYSCRNRMQFHLAPSGGIGLKRRAGTEVVETPTCPIAD
                     RPIRRWMEAREGRGRAREDLRSYLEGKDRFLVFADGRAGDDGRTEPGDARVYLEGRDG
                     EVRVPVAGREIAFHVKGFFQSNLRALDTLAPSVVQGLSGSRAADLYCGVGLFGSFLRD
                     SFEVLVCVEHNPFALEYARRNAAGRTEYHALAVEEWVKSPSARKGFDAAVLDPPRSGL
                     APTVRDWLADARIPSLSYVSCDPATLARDLKDLTGRGYRIESIGMHDFFPQTGHLETH
                     VRLRWD"
     gene            10740..12362
                     /locus_tag="P5313_09380"
     CDS             10740..12362
                     /locus_tag="P5313_09380"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY80616.1"
                     /translation="MNTGDVLLVGRFYSLAREGERFSALRVGTDGRISQAYPDGAPLP
                     PDIPRRPLPSGTTACVPAFVDSHAHFLTKAAMGALVKTLSRLEDGRVLPDCLDGVRTL
                     LAEASEERPRGPVIGYGLAPGVLAERRLPTAAELDAWLPGRPVIVLSFDGHSSSYSTA
                     ALRILGLESQARDGVLSGEAHEFNMDRVMTYAMRALSPAALAKGLSRAVREAAEAGLA
                     AVDCMEGFGNERREPGTEILARIGGRLPLRLRLWLQYTRPELVGRYVPRMARPRVGGC
                     LHWEMDGSISSRSAAMDRPYLDRDHSGRLYRSPEEAYALAKPFYNAGYQISAHAIGPR
                     GIESILSAYEKLSAGLGDRARDRRHRIDHFEFPRPDQVERAGRLGLVLPVQPGFAWID
                     ARFHRSYPEALDDQTFASFTPLRSLLQAGAVPALSSDAPVQPFDPFLQIAGAVEHPVA
                     SESLPVYDALRAYSWAGAYGAYEEEDRGTLEAGKYADFAPLDRDPFETPKAELYRIRT
                     LGTWIGGRPLESPPDNPAGFVMRALTSPRRKI"
     gene            12359..13531
                     /locus_tag="P5313_09385"
     CDS             12359..13531
                     /locus_tag="P5313_09385"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HRY80617.1"
                     /translation="MSVPARTLSFPEATAIIAGYGIGGGMLALPYLVSLNGIGPSALV
                     LAGAYALSLLLHLMIAELAAGDGSGRQIVELFREYLFTGKGGKALTWVFFGVMGVVFV
                     ANLAAYVSGGSEILAALGLAEPWGGIAFYAAAAAVAAFGLKVLGVAEKWAVGAMAVLF
                     ALLVAASLVALAKADFTIPAPAPALSPNAAGLLALYGMAMFCFAAFFSVPQAAQGLAD
                     RPTLIPRAVAAGLGVNFTLILLVTTLSLLLSPEPTIIATVGWGRALGPRAELAGTGFV
                     LLAMLTSYWSISFALSSMIRERLGLGNFPAWLLATVPTLVLALAGFGGFLEFMRTAGG
                     AIAVLVAVLFVPAYRRYRNRKGTPVILPPGISGRAWDWLAVAAYLAMAAGSLMPIR"
     gene            complement(13555..>14230)
                     /locus_tag="P5313_09390"
     CDS             complement(13555..>14230)
                     /locus_tag="P5313_09390"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRY80618.1"
                     /translation="TVQAVMTHRIDVFSLDGRTTAQEAATAAVGELHSRFPVYDEDPE
                     KIVGVVALKRVLDEVVHGRRDRPIREIMAEPVLVMSTKPLGELFDLLKRRKESFAVVL
                     DEYGGLAGVVTIRDVIEEIVGEIYEDGDEEPRERLRRRADGSYTVSGDAPLSLVADVT
                     GTESPGTVDIQTVAGYVAWILDRIPVEGDVAHTPLGRFTVLKVEANRVEEAVFRPAPQ
                     EVSETE"
BASE COUNT         2297 a         4885 c         4757 g         2291 t
ORIGIN      
        1 aggaggggcg gcccggccgg gccctccgcg gcggactccg aggtccccgg ttccgcgggt
       61 cccggctcag acgaggtcgt ggcggtcccc gtacttcacg atccggacgt cccggatacc
      121 cttcccgccc aggaaggagg acatcgcggc caggctctcc gtctcgccgt ggaccaggaa
      181 gacccccttg agccgggaga ggtccagtcc ggacatccag tcccaggtct ccttgtagtc
      241 cgcgtgggcg ctgaaggcgt tgatctcctc cacccgggcc cggagtttga ggatgttccc
      301 gtggatccgc acctccggca cccgctcctt gatccggcgg cccagggtat ccgcggccat
      361 gtatcccacg atcaggaccg tgttggccgg gtcctccacg gtatgcagga ggtggtgctg
      421 gatccggcct gcctcgcaca tcccgtccgc ggagatgatg atggccgggc ccttcaggtc
      481 gttcagggcc ttggattcct ccacggagga ggagaagcgc aggtggttga atccgaaggg
      541 gttcttgtgg tgcttgagga aggcgtcgtg ggtctcccgg tcatagcact ccggatggac
      601 ctggaagatg gtggtggcgt tgatggccat gggcgagtct acccagatgg ggatctccgg
      661 gatggccttc cgatccgaga gaaggtggag gtagtagacc agttcctggg tccgctcgat
      721 ggcgaaggcg ggaatgacga ccttgcctcc cttggcggcg gtctcccgca ccacccgggc
      781 cagcttttcc agggcctcgt cctgggcctc gtggagccgg tccccgtagg tggcctccag
      841 gaccatccac tccaccgggg ggatcctgtc cgggtcccgg atgatgggct tgcccttccg
      901 ccccaggtcc ccggtgaagg cgatggtggc ggagcgcccc cctgaatcct tggcctccgc
      961 cacgatcatg gaggatccca ggatgtgtcc cgcgtcgaag aactcgatcc ggaccccgtc
     1021 ccccgcggac aggggccggt tgtaggatac ggtcacgaac tggcccacgg cctgaaccgc
     1081 gtcctgctcg tcgtacaggg gcttccaggt gaagggctcg ccccgcttgg ccgcctgctt
     1141 ggccaggtac tccgcgtccc gtgcctggat gcgggcggag tccatcatga tgatccccgc
     1201 caaatcccgg gtggcggggg tcgcgaagac gttcccgcgg aagccccgct tggacaggag
     1261 gggaagcatg cccgagtggt cgtagtgggc gtgggtcagg aagaccgcct ccaggcccgc
     1321 cggatcgggg accagggcct tgttcttctt ttccgcggtc tcgcgcttgc cctggaaggc
     1381 cccgcagtcg accaggaaac ggcggccgtc gatatccagt acgtgcttgg agccggtgac
     1441 ttctcccgcg gctccctcgg aataaaaggt tatcatagga agaatataaa cccggatacc
     1501 ccggccggaa agaccttgtt acaggaagaa gaccacggtg cccccgaccg cgacgcaggc
     1561 ccccaggatc tcccagaggc tcaccttctg cttcaggaag aggaccgagg gcgggatgat
     1621 gaggaccggg gccaggccga tcagggtgga aaccgtgccc gcctgggtat ggttcagggc
     1681 gaaaaccgag agggtgaccc ccaggaaggg gccgaacacg gcccccgccg cggtgttccg
     1741 catcccggct ctgtccccgg gggcctccac gaagaccttg cgtcccttgc cggagaccag
     1801 ggccaccagg gcgaacccgg ccatgccggc caggacccgg atctgggtgg ccgccacggt
     1861 atcgtagccc gcggagccca gcttggtcag gatcatcccc gcggactgcc ccaccgaggc
     1921 caggaaggcg aaaagatagc ccttccggcg tttgtcccga tcctggacga gttcggcccg
     1981 gggatcccgc ggccgaccca ggaccgccgc cgcgatgccc gcgcagacca gggccatgcc
     2041 cgcgaggccc cggggcggca tgagctcccc gatcgccagg aaggagaaga aggccgtggt
     2101 ggggggggac agagccagga aaagcatggt cgtccgggat ccgatcatca cgtaggcgtt
     2161 gaaaaggaag atgtccgcga tcacgaagcc caccaggccc gaggcggaca gccacagcca
     2221 ggcccgcccc ggggcgtccg tggggaaggg aaggccccgc aggatccagg ccgtgccgga
     2281 aagcaggagg atggccccca cgagcttata gaggttgacc gccacggtgc cgatgcgccg
     2341 ggaggccttc tcgaagaata ccgccgagac ggtccaacag atcgccgtcc cgaaggccgc
     2401 gagttcgccg atacgctgca tgatgggcga tcctataccg gcccccgagg gcatggatag
     2461 cccccggagg tttgacttcc cgcccggcag gtcctatgtt tgtacatgga ggatcatcgg
     2521 acgtcatcgc cggaccggac ctgggttcgg aagggaggag acatggccgt gatttgcata
     2581 gcccgggaac tcgcgtccct gggggaggag accgcccacg aactggcgga cctgacgggc
     2641 taccgctacc tggacaaagc ctacgtggag aaaaagctgg cggagtacgg tatcgccgcc
     2701 acggagatcc agaaattcga cgagaagaag cccggcttct gggcttccct gagccggggc
     2761 cgggacgact acctgcactt cctgaagacc gtgctgtacg aggaggccca gatcggggac
     2821 tgcatcctgg tcggccgggg agggatggga atcttcgccg gggtgccggg ggtgatcgcc
     2881 gtgcggctca cctcgcccct gcccgtccgg atcgagcgga tcaagaagtt ctacaactgc
     2941 gacgagcgcc acgccgagca gctggcccgg cagagcgaca acgaccggga cggcttccac
     3001 cggtacttct tcaacctgga ctggagggat cccgtcaact acgacatggt cctgaacacg
     3061 gggctggagc accccgccac ggtggcccag atcatcaacc agtaccgcct cctgaccatc
     3121 acgaaggatc tggagtccaa gtgcggacag cgcctgcggg agctctccct gggccagaag
     3181 gtcatcacct cggtcctctt cgacaagggc atctccatcc acttcctgga ggcggacgtg
     3241 aagggggacc tcgtgaccct ccacggcgtg gccaacaccc agcagaccat cgacgcggcc
     3301 gtcgcggcgg cccacgagat ccccggggtc ggccacgtgg agagcgcgat ccaggtagtt
     3361 caggagtata ccgtgatgcc ctaacgggca tcacggtgat cggaatgtcc ctgcgggaca
     3421 ttccggctgt caaggaacat cacggtgagc ggtatcgagg cgaagcctcg ataccggctc
     3481 gcaaccaccg ggcatgacgg tgatcggaat gtccctgcgg gacattccgg ctgccaagga
     3541 acatcacggt gagcggtatc gaggcgaagc ctcgataccg gctgccctga acggacctat
     3601 cggccgattt ccggttgcgc gggcgccctg gccggggtat ccttccggaa tgcagcttct
     3661 cacgggattc cacgccatcg aggaggccct gaagcggggt caggtccgag gctacctgcg
     3721 ggtgtccggt accggccccc ggatcaagcg catcctggag ctggcggaca agatcggcct
     3781 ccgggtcgag aaggccaagt ccggggacct ggacgccgcc gccccggaca accggggcat
     3841 cgtcctgacc ctggagggcg attcccggcc ggccaacgac gcggacctgg acgaattcct
     3901 ggaaaagctc tccaagcccg aggcccttgt cctcctgctg gaccacgtca cggatcccca
     3961 caactacggg gccatcctcc ggtccgccga cgtcctgggg gcggacctgg tggtcgtgcc
     4021 ggagcggcgg agcgcccacg acacggacac cgtggcccgc gcctccgcgg gggccgtggc
     4081 ctgggtgccc gtggccgtgg tgcccaacct ggtccgggcc ctgacggccc tccaggagaa
     4141 cggcttctgg ggctacgccg cggacatggc ggggatcgag atcggccagg ccaagctccc
     4201 ggagcgaacg gccatcgtcc tgggcagcga gggctacggg gtgtcccgcc tgctcaagga
     4261 gacctgcgac ggctccgtga gcatacccca gcggggcaac gtcgagtccc tgaacgtctc
     4321 cgtggccgcg gggatcgtga tgtgggagta ccggagacgg tacgcgggct gatcacagat
     4381 cccagatctc cccgaactcg tgctcccggt cgcagtactg acaggtgaag atccgctccc
     4441 cggtgcggcg gaaacgggcc ggagcgccct cccgatggga ggggtgggag acgcaggctt
     4501 cgttcttgca cgagatctgg tcgaagttgt agatccgggg gggcatggcc aagcggtact
     4561 tcttgaggac ccgggagtcc ttgatcagat taagggtgca gcccgggacg atggccccga
     4621 gcttcttgag gtcccggcgg tcgaaggcca ggatgtccgg cagggagagg atgcccttgt
     4681 agacctcggg ccccgcgttg gagtggtaga ctccgtggga ggaccggacg tccaggccca
     4741 ggatgcggcg gaccttgtcg acggtatccc agatctcccg gatcgggcgg cccgcggcga
     4801 tatggtcgat gacgatcccg ctctccacgg gttttatgcc caccttgtag tccggcttga
     4861 cccgaggcgt caccttggcc tcggtcacga agtcctcctc gaacctcagg ggttccttgg
     4921 gcaggccccg gaagtccccg cccagggctc cggacaccag gcccaggagg gcgatgcgga
     4981 cgaagtagcc gttggcggat tgggcgtccc agccgttcag gggcagctcg tccaggaaga
     5041 cgggaatctc gggggccgtc cggtcccggg gcaggggatg gtagaaccgg gttccctcgg
     5101 gcaggcggct catcatgtcg ggccggaagg tgacggtcct gcggagctcc gcggcccggt
     5161 ccaggaccct ctcccccatg cgctccagct ggagccgggt gtagtaccag atcggggcga
     5221 cccggccgga ggccaggtat tcgtccaggg actcgtaggc ccggacctcg aagcccgagg
     5281 ccttcatccg ctccacgtag gcggggggca gtgccaggag ttccggggcc accaggtcca
     5341 cggaaacctc cttgaagatc cgcagcccct cggccttgga atggacggtc cgtccgtgcc
     5401 agaggtcccc caccagggcc aagtggatcc gggagtcgtc cccgccccgg tgctccagga
     5461 aggtgaactc gtccaggaat tcctgggtgg ggtgctcgtg cttgccgtcc ccggcgttca
     5521 ggaaggcggg tttggggaag cccgcggact cgcagtaggt ccccacggag gagtcgagcc
     5581 agcggcagac tccctccagg cccgagcgga tgacgaagca ggactcggcg gcgtatccga
     5641 ccagcatctt ggccgcgtcc gccaggctct cgttcttggc gaaggaggag gtggcggcgt
     5701 cgaacatgtt cacccggcag ccgtggaact ccgcggcgtt ccggaaggac tcccgggtcc
     5761 gggtggagtt ctctaggaag atgaggtagg tctggtaatc cggatccgag atccggaaac
     5821 gcgaagggtc ccgcccggac cggatcgtat ccttgatatc ccgggccagg gagtacaggt
     5881 accgctgctc gtccccggaa aggtcgtcca ccaccgcgat cgtcctgccc cggaagggtc
     5941 ccgtccgttc caccttcgtc ctccttctgg tttcaccggt ccgcataaaa aaagctgtcc
     6001 aaaaagctca ttgagctttt tggacagccg cttccaagtc ggcaggttcc gacgttctcg
     6061 ccgagaacgt cggaacctgc gaggtgagtc cgatacgtac ccgacgtatc ggactcaccg
     6121 gccgccttcc gcatcaccgt cggatctcct tgacggtctt caggtccgcc ttgttcagca
     6181 ggatgaggcc tccccccgcc ccggcgacca cgagtccctc ggggacggac ctcaggaagg
     6241 tgaaggggct gaccgcggcc tcggacttca tcttgagctc caaggtcgcg ggatcgaata
     6301 gcccgaggac ccacttcccg ccgtcgttag ccaccgccag gatgccggca ccctccttcc
     6361 agacccccgt ctcggggtgg acgtccacgg tcccggtgac cttggcggag agagtctcca
     6421 ggtccaggag cacgaggcga acggccccgg agccctcggc ccggccggcc acggctacga
     6481 aggctccccc gatgtccagg aggctgtccg cccggagggt gttgatctcc ccggtcttca
     6541 ggtatttccc gccggccagg tccaccaggg ccagccgggc gtaggggcgg ttcgggtcca
     6601 ccaagtccaa gaggaccgag ccccgcggag cggcctcggc cttggccgcg acctccttgg
     6661 ctatctccgc cttctggtct tccgccacgg ccttgcggtc ctcggcgatc tccgcctcct
     6721 tggcggccac cgcggcctcc tccttggtgg tctcctcctt ggccgcggcg acggcggctt
     6781 ccttttcggc cgcggccttc tcctgggccg cgacctccgc ctcccggcgg gtaagctcgg
     6841 cctcctgctc ggggccctgg gcggaagctc tgtccctctc caactccgcc cgggccgcct
     6901 cggccctcgc cgtttcctcg gctgcggctt tttcctgctc cgcgatccgg gccttgtcct
     6961 cctcgagccg tgccttgtcc tgctcgacct cccgttcctt gaggtccacc atctcccgcc
     7021 gttccgggag agccgcggta ccggactcct ccttgaggct ctccaccacg gacttgtccg
     7081 tgatgggccc cgtgtccacc cggccgggag tcccggcccg agcggtgatc ggaaggacga
     7141 tgcggctgcg tccggcccac tcgtcccagc ggaccgagag tccggcgttc tcggccgtca
     7201 ggtgggtcag gaccgcgggt ttgtaggcct tgccgaaata ggccaggtcg ccccggtaca
     7261 cggcgttgta caccgtgagg aaggtggcaa ggaggaagcc gtccgaccgg gagtagccgt
     7321 aggcttcctc caggtaggcc cccaggatgt gccggagatt ccggatgtgg tccaccccgg
     7381 cgtcgcgtcc caggatgatg atgtccgcgt ccaggcccga gggcgcctgg ggatcccgca
     7441 cccggatgac cgcgtaccgg gtctcgcttc ccgcccgagt cgccccggcc cgtacggccc
     7501 ggccgagggc ggcccccagg ccccggatct cgtccaaaga atcgattcgg gcgtggggtc
     7561 ccacgtagtt gatgaactcg atggtccttc cgcccacgga gcggagctcg tcctccgcca
     7621 cggtctgggc ggaaaggatc gccccggccg tcaggaaggc gaggagggcc gggagggcga
     7681 ggtatttttt cacgattcct cctattcaac gcgcaggttc ggagaggcgg cgcagggccg
     7741 ccagggcccg ggaccgcacc gcggatccgt agggtttgtc cgccagcctc aggaccgccc
     7801 ggactcctcc gggatcggac aactccccca gggcgcggta gatcccctcc accgcgtcga
     7861 tcgtggccag ggcgccgggt tcgtccagga agaactcccg atccgccgcc ccctggaagg
     7921 ctcggagcgc ctcccccgtc aggtccaggc cgatgatccc gattgcccgg gccgcggagg
     7981 cccgcaccgc gggctcggga tcccggaggt aggcctccgt caggaccgga accgcccggg
     8041 gggaacccaa catgcccagt atatcgcagg ccagggcccg gggaagggcg cccgacggcc
     8101 gcggtccccg gggagccgca cctccgtaca cggaagcctc acgagccccg tagcccaggg
     8161 cgatcgcgga gcagagaagc agagcgtccc cctcctccgc tccaatacta gcggatccca
     8221 gggatttttc aatacgccgg agctctcgaa gcgccgcgtc gtccccgaat cccggattcc
     8281 agaagatctc ctcgttcgcc cgggcacggg cggccgcgaa gtccacgtag cccggtgccg
     8341 gccgtgcggg ttcgcccaga ggcccctcga agcggtaggc gtagaggatc cagtcctccc
     8401 cggcggaaag tacgatgccc gaggaggtga ccgcgggcat acgtggagca tggcggaggg
     8461 acagagctcg atagacttgt ccgtccttgg ccaggctggc ggctccccgg ggtcccagga
     8521 ccaggacccg ctccgggaag acccggatgt cttgggcgtc taaaataccc gtggacgtcg
     8581 tgtcgcttac ggtaccgccc tcccgtaccc ggactacgct ccccccggca agcaggagcc
     8641 aagcttcagc tctgtccccc gccgcggcca cgaccggagc ggccaggcgg actcggtagg
     8701 tctcctgtcc cgcaccgtcc cgtaccgata cggccccgtt ccggtaagcc agcacgaacc
     8761 cctccccgat accgcacaaa gccgaggggg gctcggagac ggggacagag ctcgaggttc
     8821 catcggggac agagctcagt agagtcccgt cctcggcata ctcccggatg ctcccgtccg
     8881 ccagtgccag ggtgacccgg gtctttccgg agggagtgaa gcggacggag ggtcccgccg
     8941 ctatcggggc cgcatgttcc acggaccaca ggagccttcc cgaagcggac catgccagaa
     9001 ggcgggaagc cgcggcggcg aagaagcggc cgtccgcccc gaaggccggg gcccgcacca
     9061 ccgggacccc cggccggact cgccagactt ccagtccagc ccgattcacc atgaccaggg
     9121 agccgtccgc ggctggcaga agggcccggc cgaagggatc cggggctatc catgccgggc
     9181 tcccgtccct ccaggccctt cgccccacgg cgcggcccga gtcgtccaga atatacaggg
     9241 tgcgatcctc ggagacgatc cagaccctgc ccgcctcggc cacgggaccc gcggcggcgg
     9301 cgcccgccag ggtgaagcgg aaggccagga tgtcctggac cggggactga gcgttcgagg
     9361 gaacggcaag aatcgcggcg gccgcgaaaa gggcaatcgg atatcgggta cgcccgcgga
     9421 cccgacgcgg ctcagtccca gcgaaggcgg acatgggtct ccaggtggcc cgtctgggga
     9481 aagaaatcgt gcatcccgat cgactcgatc cggtagcctc ggccggtcag gtccttgagg
     9541 tcccgggcca gggtggcggg gtcgcaggaa acgtaggaca gcgaggggat ccgggcgtcg
     9601 gcaagccagt cccggaccgt cggggcaaga cccgagcggg gagggtccag gaccgcggcg
     9661 tcgaagccct tccgggcgga gggcgacttc acccactcct ccaccgccag ggcgtggtac
     9721 tctgtccgac cggccgcgtt gcgtcgggcg tattccaggg caaagggatt gtgttccacg
     9781 cagaccagga cctcgaagga gtcccggagg aaggagccga acagccccac cccacagtag
     9841 aggtccgcgg cccggcttcc cgacaacccc tggacgaccg agggtgccag ggtgtccagg
     9901 gctcggaggt tgctctggaa gaaacccttc acatggaagg cgatctcccg gcccgccacg
     9961 ggaacccgaa cctccccgtc ccggccttcc aggtagaccc gggcatcccc gggctcggtg
    10021 cggccgtcat cgccggcacg gccatcggca aagaccagga accggtcctt gccctccaga
    10081 taggaccgca ggtcctcccg ggcacgaccc cggccttccc gggcttccat ccaccgccgg
    10141 atgggccgat ccgcgatcgg acaggtaggc gtctccacga cctctgtccc cgcccggcgc
    10201 ttgaggccga taccgcccga gggtgccagg tggaactgca tgcgattccg gcaggaatag
    10261 ggttccgaag gaacctcatc gatatcccgg acggagaggc ctccgatgcg ggaaaggcag
    10321 tcccttagga tctccgcctt ttcgcggacc tgccgggaat acgccatatg ctggagcgag
    10381 catccgccgc agattccgta aagcgcgcat tccggatcta tccggtccgt ggaagcctcg
    10441 agaacctccc gtagctccgc ggctgcccaa tccccggaag ccctgacgcc atctgggaag
    10501 gccgcacgga cggtctcccc cggtacagtc ccaggcacga agaccgcctt cccctccggg
    10561 aaggccagcc cgtcaccccc gacggcgagt ttctcgatcc gcagctcaac gatatcccgg
    10621 tcgctcatat cgaggatcga gtatacccgc cgccgggagg accgtaaaga ttgacggcga
    10681 gggacgcacg gcggcggaaa ccttgacaga ggccccggtc cggcccacac tcgaggcgga
    10741 tgaacaccgg agatgttctt ctagtcggac gattctacag cctggcccgg gagggcgagc
    10801 ggttctccgc cctgcgggtg gggacggacg gccgcatatc ccaagcctac ccggacggtg
    10861 cccccctgcc gccggacatc ccccgccgcc cccttccgtc cggtacgacg gcctgtgtcc
    10921 ccgccttcgt ggacagccac gcccacttcc tgaccaaggc ggccatgggt gccctggtga
    10981 agactctgtc ccgattggag gatggccggg tcctcccgga ctgcctggac ggggtccgga
    11041 ctctcttggc cgaagcctcg gaggagcggc cccggggacc cgtcatcggc tacggcctgg
    11101 cgccgggggt cctcgcggag cgacgccttc ccacggccgc ggaattggac gcatggctgc
    11161 cgggacggcc ggtcatcgtc ctttccttcg acggccattc cagctcctac tctacagcgg
    11221 ccctccgcat cctgggcctg gaatcccagg cccgggacgg cgtcctgtcc ggggaagccc
    11281 acgagttcaa catggaccgt gtcatgacct acgctatgcg agctctgtcc cccgccgccc
    11341 tggctaaagg actttcccgg gcggttcggg aggcggcgga agcaggactt gcggcggtgg
    11401 attgcatgga aggtttcggg aacgagcgcc gggagccggg cacggaaata ctagctcgaa
    11461 tcggcgggcg tctacccctc cggctccggc tctggctcca gtacacccgg cctgagcttg
    11521 tcggccgcta cgtccccagg atggcccggc ctcgggtcgg aggctgtctc cactgggaga
    11581 tggacgggtc catctcctct cggagcgccg cgatggaccg gccctacttg gaccgggacc
    11641 actccggcag gctctatcgg agccccgaag aagcctacgc cctggcgaag cccttctata
    11701 acgcgggcta ccagatctcc gcccacgcca tcgggccccg ggggatcgag tccatcctct
    11761 cggcctacga aaaactttcc gcgggactcg gggacagagc tcgggaccgg cggcaccgga
    11821 tcgaccactt cgagttcccc cggccggatc aagtggagcg agcgggccgt ctgggcctgg
    11881 tcctacccgt ccagccgggc ttcgcctgga tcgacgcccg gttccatcga tcctaccccg
    11941 aggccctgga tgaccagacc ttcgcctcct tcacccccct gaggtcccta ctccaggcgg
    12001 gggccgtgcc cgccctttcc tcggacgccc cggtccagcc cttcgacccc ttcctgcaga
    12061 tcgccggagc cgtagagcac cccgtggctt cggaatctct tcccgtgtac gatgccctcc
    12121 gggcttattc ttgggctggc gcctacgggg catatgagga ggaggaccgg ggcactctgg
    12181 aagcagggaa gtacgcggac tttgcccctc tggaccggga tcccttcgag actccaaagg
    12241 cggagctgta ccggatccgt accctgggga cctggatagg aggccggcct ctggaatctc
    12301 ccccggataa tccggcgggc ttcgtgatgc gcgccctgac atccccaagg aggaagatat
    12361 gagcgttccc gcccgaacct tgagctttcc cgaggccacg gccatcatcg cggggtacgg
    12421 gatcgggggc gggatgctcg ccctgcccta cctggtctcc ctgaacggga tcggcccctc
    12481 ggccctggtt ctggcgggcg cctatgctct gtccctcctt ttgcacctca tgatcgcgga
    12541 gttggccgcg ggagacggaa gcggccggca gatcgtggaa ctctttcggg aatacctgtt
    12601 taccgggaag ggtgggaagg ccttgacctg ggtcttcttc ggggtgatgg gggtggtgtt
    12661 cgtcgccaac ctggcggcct atgtctccgg gggcagcgaa atcctggcgg ccctggggct
    12721 ggcggaaccc tggggaggaa tcgccttcta cgccgcggct gccgcggtcg cggccttcgg
    12781 tctcaaggtc ctgggggtgg ccgagaagtg ggccgtagga gccatggccg tactcttcgc
    12841 cctgctggtg gccgcctcgc tcgtcgcctt ggcaaaggct gacttcacta ttccggcccc
    12901 cgccccggct ctgtccccga atgcggccgg gctcctagcc ctttacggga tggccatgtt
    12961 ctgcttcgca gccttcttct cggtgcccca agccgcccaa ggtctggcgg accggccgac
    13021 cctcataccc cgagccgtgg ccgcgggcct gggggtgaat ttcaccctga tcctcctggt
    13081 gacgaccctc tccctccttc tgtctccgga gcccaccatc atcgcaacgg tcggctgggg
    13141 ccgggccctg ggcccccggg cggagctcgc gggaacgggc ttcgtcctgt tggccatgct
    13201 gaccagctac tggtccatct ccttcgccct gtcctccatg atccgggagc ggctgggcct
    13261 ggggaacttc cccgcctggc tcctggccac tgtccccacc ctggtcctgg ccctggcggg
    13321 ctttggaggc ttcctggagt tcatgcgaac cgcggggggc gcgatcgccg tgctggtggc
    13381 tgtcctcttc gtacccgctt accggagata ccggaaccga aagggaacac cggtcatcct
    13441 gcctccggga atctcggggc gggcctggga ctggctggct gtagcggcat accttgccat
    13501 ggccgcggga tccctgatgc ctatcaggta ggcgcgggga cagccgaggc actctcactc
    13561 ggtctcggag acctcctggg gggcgggacg gaataccgcc tcctccaccc ggttcgcctc
    13621 cactttcaag accgtgaacc ggcccagagg ggtatgggcc acgtctcctt ccacggggat
    13681 gcggtccagg atccaggcca cgtagcccgc gaccgtctgg atgtccacgg tgcccggaga
    13741 ttccgtgccg gtgacgtccg cgaccaggga cagaggtgcg tccccggaaa ccgtatagga
    13801 cccgtccgcc ctgcgccgga ggcgctccct gggctcctcg tcgccgtcct catagatctc
    13861 cccgacgatc tcctcgatca cgtcacggat ggtcacgacc ccggccaagc ccccgtactc
    13921 gtccagcacc acggcaaagg attccttgcg ccgcttcaat aggtcgaaga gttcgcccag
    13981 gggttttgtg gacatgacca ggacaggctc ggccatgatc tcccggatgg ggcggtcccg
    14041 acggccgtgg accacctcgt caagtacccg cttcagggct accacgccta ctattttctc
    14101 gggatcctcg tcatacacgg ggaaccggga atggagttcg cccaccgccg ccgtcgccgc
    14161 ctcctgggcg gtggtacggc cgtccaggct gaagacgtcg atccggtggg tcatcacggc
    14221 ctgcacggtc
//
LOCUS       DAOTEI010000085        14195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2963_length_14195_cov_4.360891, whole genome shotgun sequence.
ACCESSION   DAOTEI010000085 DAOTEI010000000
VERSION     DAOTEI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_14195_cov_4.360891"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="P5313_09395"
     CDS             complement(<1..290)
                     /locus_tag="P5313_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80619.1"
                     /translation="MNVFREPIEKGDITSEAGLKRTFRALALRLHPDTCLAGDAAERF
                     LRLRRDYDEALRERGWHRPAPAPGEPFSFEACVDLFGELVGGNFPADPRVGRT"
     gene            complement(319..1104)
                     /locus_tag="P5313_09400"
     CDS             complement(319..1104)
                     /locus_tag="P5313_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80620.1"
                     /translation="MADEKHLYLIIHPNHSLIASGLEPEHFAKHYVQGSTRYFEGRLI
                     FVEIDPAFRHPYFDIDSAFSQLVPHEDGRPKATKFIKSYRTLEHVDYSALGQLFICNS
                     LGEFVALESSEYDPKVEGDDLSIILEINPIKMMVLTKFNFIEYSRYITDPHQPKGAPC
                     MFYTQLDFNADDFLAEFDENPFIRCFVPGIHPARLRAAIQEVRTTPGKNVKGLSLDCP
                     YDKISYKFLRHGFMFAAQSGYKYYPLKSLEDVERDWYKFWKNL"
     gene            complement(1125..3200)
                     /locus_tag="P5313_09405"
     CDS             complement(1125..3200)
                     /locus_tag="P5313_09405"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRY80621.1"
                     /translation="MPSLLIAVAAVGGGLAVLYAFLKARWIYRIKVGNATLVRISGYI
                     AEGAEAFLTREYKVLVPFVAIAALFLAVANSGPLRLQALSFALGALCSAAAGWFGMKV
                     ATAANSRTAQAAETGIDGALRVSFAGGSVMGMTVVGLAMLGISAVLAAGLRLFGESAG
                     ALHDVVFPILSGFSLGASTIALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNPA
                     VIADNVGDNVGDVAGMGADLFESYVGSIVGCVVLGANVMNATDTVKMQLSFLPLALCA
                     IGILASVAGTFLVRAKPGKSPQKALNTGTFGAAILATALLYPVIALILGDSTFEKGYG
                     ALHIFLAAVLGLASGVGIGTLTEFFTGTTTKPVLKIARACDTGSATAIISGLGVGMLS
                     TLPPTLVIAAAILGSFQLAGLYGVGIAALGMLLTLGIQLSVDAYGPIADNAGGLAVMS
                     ALKKEVREITDSLDAVGNTTAAIGKGFAVGSAALTSIILFSAFVQATGAAETLDLSIV
                     SVPVLVGFLIGGMIPFLFSSLAMDAIGISAFAMIEEVRRQFREKPGILAETETPDYRA
                     CVDISTLSALKKMILPGVIAAVSPVLVGVLGGIPMLTGLLAGVTLTGVLLAIFMSNAG
                     GAWDNAKKLIEGRPGGAGKGSGAHKAAVVGDTVGDPFKDTAGPALNILIKLMSIIALV
                     IAPILRDLR"
     gene            3388..3828
                     /locus_tag="P5313_09410"
     CDS             3388..3828
                     /locus_tag="P5313_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRY80622.1"
                     /translation="MTIRVVYWSGTGNTKAMAEAVAEGARSAGAEAELSEVGSADVRS
                     VLAADAAALGCPSMGAETLEEEEMEPFVRSLESAGLEGKPVLLFGSYDWGDGQWMRDW
                     EERMRKAGAALAGGGFIVNLTPSASDIVALAEAGRNLAAAVSGK"
     gene            complement(3871..5178)
                     /gene="hisD"
                     /locus_tag="P5313_09415"
     CDS             complement(3871..5178)
                     /gene="hisD"
                     /locus_tag="P5313_09415"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRY80623.1"
                     /translation="MDRNPLRLRRLRAADLERKPASIPDPVTADRAAAIVADVRERGE
                     AALLGYARELDGLGEGPLYLGPRDFRKALDALPRDSRELLERAGARIGAFAKAQAASL
                     SALEVPVPGGFAGHEVLPVETAGCYAPGGRYPLPSSVLMTALTAKAAGVGTVWTASPR
                     PARETLAACAVAGADGLLAAGGAQAIAALAYGAGPVPPCARVVGPGNRWVAAAKRLVA
                     GDTVIESVAGPSELLVLADDGADPEIAAADLLAQAEHDPDAVPVLACRTEAFAARVEE
                     ALERRLADLPTGEVARRALDNGGVLVDPDLKTLVRAADAFAPEHLEILCEEPEALARK
                     IRNAGAVFLGAGTAEVLGDYGTGPNHVLPTGAHARLAGGLSALSFLRVRTWIRIDDPA
                     DASELYRDAAAFARMEGLEAHARAGEVRGRRPVDSGRKAPAGA"
     gene            complement(5160..>6256)
                     /gene="hisE"
                     /locus_tag="P5313_09420"
     CDS             complement(5160..>6256)
                     /gene="hisE"
                     /locus_tag="P5313_09420"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRY80624.1"
                     /translation="GGIRDYETAVRLLDAGARKVILGTAARPDLLSRLPRDRVIAALD
                     ARNGEVVVEGWRTRTGASVADRIRELAPYVGGFLVTTVEREGRMAGADLAAAADVVRI
                     ARECREDLRITWAGGVTSAEEVAELDRLGADAQVGMALYSGRLSLAEAFTAPLASDRP
                     DGLWPVVVCDEAGIALGLVWGDRESVAESLKRGRGVYRSRTRGLWEKGASSGNTQDLV
                     RIEVDCDRDALRYVVRQNGGGFCHLGTRTCFGPDQGLGALDRTIRGRQESSPPGSYTH
                     RLFTEPGLLSAKIREEAAELAEAEGPVRAAEEAADLVYFALVKLRAEGASLADVERIL
                     DRRALKVVRRPGNAKPAFQEEGGTRWTGIH"
     assembly_gap    6257..6266
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6267..6479)
                     /locus_tag="P5313_09425"
     CDS             complement(<6267..6479)
                     /locus_tag="P5313_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRY80625.1"
                     /translation="MIVPSIDLMGGRAVQLEGGETLRIDAGDPRPKAAEFSRVGELAV
                     IDLDAARGEGDNRRLVEDLVSAYPCRV"
     gene            complement(6476..7954)
                     /gene="hisF"
                     /locus_tag="P5313_09430"
     CDS             complement(6476..7954)
                     /gene="hisF"
                     /locus_tag="P5313_09430"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRY80626.1"
                     /translation="MRTAVVRTGTANLASVLAGLRRAGAEPYVTEDPGDVLREGRVIL
                     PGVGSFGAALAELRSRGLDRALAERFRAGRPLAGFCLGMQLFFEASEESPGVRGLALL
                     PGTIRKYPDTVRTPRLGWGAVLPDPGSGCLEPGWAAFANSYRLVEPPPGLRTAWADYA
                     GPFAAALERPGLLLCQFHPELSGAWGKALLSRWLEGAGAADGLTAEGPGARVSRPPAE
                     PLSGTRSVRVIPCLDVDAGRVVKGTRFLDLKDAGDPAELAARYEGEGADEIVLLDITA
                     GLEGRATRADTVRRVRAALGIPLTVGGGIGSVADAAVLFEAGADKVSVNSKAVRDPAI
                     LTELAERFGVQAVVIAVDAARRPDAARRPDAAGRLSGARPEGASPWEVTVDAGRTRTG
                     LDPAAWARRAVEAGAGEVLLTGRDRDGTGLGYDLDLVSEVARAVPVPVVASGGAETPR
                     QMYEGVAAGAAAVLAAGTFHFGRRTIHEVKAYLAERQVEVRP"
     gene            complement(7951..8559)
                     /gene="hisB"
                     /locus_tag="P5313_09435"
     CDS             complement(7951..8559)
                     /gene="hisB"
                     /locus_tag="P5313_09435"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRY80627.1"
                     /translation="MSDTISVPALRRASLRRETRETRIEASLDLAPGEVKVDTGIGFL
                     DHLLSSLAFHGGFSLALACRGDLAVDDHHTAEDCGLVLGDLVRQALGDRSGIRRFASA
                     YAPLDEALARAVVDISGRPFARVDLKLARDSVGTLACENIPHVVASFATRAGITVHLE
                     VLYGDNDHHKAEAAFKALALALREAWALSGSGAVPSAKGVLA"
     gene            complement(8556..9650)
                     /locus_tag="P5313_09440"
     CDS             complement(8556..9650)
                     /locus_tag="P5313_09440"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRY80628.1"
                     /translation="MNTADERDGYRAQERPVVGYVPPARDPRIRWFLDANEGRPSRAA
                     LAALAGAATMETSRRYPRTAELEAVIAARPKAGYEPLSPSRVLVTAGGDDAIARACRV
                     FLKPGDTLVVFEPAFEMYSIGARLSGARTVGVPWPPGEPFPLEAAIETARSCRAAAVA
                     LVNPANPAGGLASPEVTEELMDALPDTSVWVDGAYADFSRTDSLAAALSRPNGLALRS
                     FSKTYGLAGLRVGWAAGTPETIAALRAAGDPFPVAGPSLAAALAARSPEGEAETSRIV
                     TRLVRERETLYRLLGDLGARPYPSETNFVLARVKDPSGLKEALASRGVAIRAYPGRPG
                     LEAMIRISGPADPEGFGVLARALRESKEYL"
     gene            complement(9647..10513)
                     /gene="hisG"
                     /locus_tag="P5313_09445"
     CDS             complement(9647..10513)
                     /gene="hisG"
                     /locus_tag="P5313_09445"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRY80629.1"
                     /translation="MDEAKVLKIALPKGRMQEGVFRLLSDAGLPIKGDERDYRPRLPS
                     PDLDAKILKPRNTLEMLHAGTRDLGFAGSDWVEELGVELTDMLDTGLDPVRLVAAAPV
                     AILEDGRLPRRPLVVASEYENIARRWIAGRDLDATFVRSYGATEVFPPDDADCIVDNT
                     ATGSTLRANGLEIVDELLRSSTRLYANPRSLEDPWKRDTIEAIVLLLRSVLTARSRVM
                     LELNVDTYKLDSVIAILPCMREPTVSPLRGGDGYAVKVAAPRDSLAALIPEIRRRGGT
                     DIIVTQPMRIVP"
     gene            10853..11659
                     /locus_tag="P5313_09450"
     CDS             10853..11659
                     /locus_tag="P5313_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80630.1"
                     /translation="MFTPLELIYPVVSRRAGGLSLGVDLFPDRKTCNFDCPYCEIFPF
                     PGEPPFRPEDLDRELAEFFEVDYPSFPPDLPLRDLTLSGSGEPTLSPHLGSALASIRT
                     ARDRWAPGADLVLITNSTTLGHPETAALLGRCVDEAGLRIWAKLDGGNEEWYGRMSRS
                     RVPFRDVVEGILSFSSAHAVIVQTMLCTLDGRGPSDADLADYAGLVEDVLGRGARFLE
                     IHLYTQARPSPEGRTSPLPEADLSAAADLVSRRAPGVPVRVFGRTGELSR"
     gene            11656..12483
                     /locus_tag="P5313_09455"
     CDS             11656..12483
                     /locus_tag="P5313_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRY80631.1"
                     /translation="MIDLHTHSTASDGTYDPEALADLAADRKLTALALTDHDSLSGLA
                     EARAEALRRGLRFIPGVEIEVAFSPGEFHLLGLDLRDTEGAIREAVGRLARSRDERNE
                     RIFERMRAAGIEGDYGELREAAAGGMIGRPHIADFLIARKVVRKKQDAFDKYLGKGRP
                     FYVAKECLELKEAVRVIYEAGGLPIVAHPLSLFVSWSRLGSLFLEWKELGIAGIEAWH
                     PTARPGHCQRLEKMARDLGFRVTAGSDFHGANRPDRRLGYTAGDRPIADSYLEALDR"
     gene            complement(12446..13684)
                     /locus_tag="P5313_09460"
     CDS             complement(12446..13684)
                     /locus_tag="P5313_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY80632.1"
                     /translation="MKGKQVQRLLFLAVFLFLFLFVARLFSPFFSILLWAGLLYVITF
                     PFYDRATRSIPDKPLGRFARKLLAGGFALGSVLAAVIPLVFLGSTLLGQARELVHSID
                     EFLERNPDFFRTLDTGGLAMRLRDTSGGLIDLTGMDLMSELSAAVTRGAETFLTVSAT
                     LLRDTAGFGVSLAFLTFTLYFFYVDGKDLIRTFVNAVPIENAYTAAFLRRFGEVGKHL
                     FRGYLLVALYQGTAAFLIFLLFGVKGPLPLGFLTAIASFVPMVGTTLVWGPVGVARIV
                     SGDPTAGFAILILSFILVSGVDNLLRPLLLTERIKIHPLLVFFSIMGGLQLFGLNGLI
                     LGPMMLMLFFTGVDLFEQAYGTRKSGRGGGESDTAGSPAGVDGDADAGTGPDAGEGET
                     EAAPEPPGVNGPGPPGTSPR"
     gene            complement(13713..14111)
                     /locus_tag="P5313_09465"
     CDS             complement(13713..14111)
                     /locus_tag="P5313_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80633.1"
                     /translation="MDILLRATIYISVAVILLWMFLGLFGPYRRRRTLLKAGDKAATE
                     DLPRTCPLCGEVLSPGQRVKTRVNPGQGDKLMQIFGCPHCYPPNIECKRICPVCEKEV
                     PRQGYVIARLFEHPGRKHVHVLGCSGCRNT"
BASE COUNT         2152 a         4908 c         5093 g         2032 t
ORIGIN      
        1 gtccggccca cccggggatc cgcggggaag tttcccccca cgagctcccc gaataggtcg
       61 acgcaagcct cgaaggagaa gggctccccc ggggcggggg cgggccggtg ccagccccgc
      121 tcccggaggg cttcgtcgta gtcccgtcgg agccgaagga accgctcggc ggcgtcgccg
      181 gccaggcagg tgtccgggtg aaggcggagg gccagggcgc ggaaagtccg tttgaggccg
      241 gcttccgagg tgatgtcacc cttttcgatc ggctcccgga acacgttcaa gcgccggtcc
      301 cccgcccgga accccggtct acaggttctt ccagaacttg taccagtccc tctccacgtc
      361 ctccagggat ttcagggggt agtacttgta cccggactgg gccgcgaaca tgaagccgtg
      421 gcgcaggaac ttgtaggaga tcttgtcgta ggggcagtcc agggacagcc ccttcacgtt
      481 cttgcccggt gtggtccgga cctcctggat ggcggcccgc agacgggcgg gatggatgcc
      541 cggcacgaag cagcggatga agggattctc gtcgaactcc gccaggaagt cgtcggcgtt
      601 gaagtccagc tgggtgtaga acatgcacgg ggcgcccttg ggctggtggg gatccgtgat
      661 gtaccgggag tattcgatga agttgaactt cgtgaggacc atcatcttga tggggttgat
      721 ctccaggatg atggacaggt cgtccccctc caccttggga tcgtactccg aggattccag
      781 ggccacgaac tcccccaggg aattgcagat gaacagctgg cccagggccg agtagtccac
      841 gtgctccagg gtccggtagc tcttgatgaa cttggtggcc ttgggccggc cgtcctcgtg
      901 ggggacgagc tgggaaaagg cggagtcgat gtcgaagtag ggatgccgga aggccgggtc
      961 gatctccacg aagatcagcc gtccctcgaa gtaccgggtg gagccctgga cgtagtgctt
     1021 ggcgaagtgc tcgggctcca ggccgctggc gatcagggaa tggttgggat ggatgatgag
     1081 gtacaggtgt ttctcgtctg ccatgttcgt ctccttatct atgtctatcg caggtcccgg
     1141 aggatcggcg cgatgaccag ggcgatgatg gacatgagct tgatgaggat gttcagggcc
     1201 ggtcccgcgg tgtccttgaa gggatccccc acggtgtccc cgaccacggc ggccttgtgg
     1261 gcaccggagc ccttgccggc cccgccgggc cgtccctcga tgagcttctt ggcgttgtcc
     1321 caggcccctc cggcgttgct catgaagatg gccaggagga ctcccgttag ggtcacgccc
     1381 gccaggagcc cggtcagcat ggggatgccc cccaggactc cgaccaggac ggggctcacc
     1441 gcggcgatca cccccggcag gatcatcttc ttgagggccg agagggtgct gatgtccacg
     1501 caggcccggt agtccggagt ctcggtctcc gccaggatgc cgggcttctc ccggaactgg
     1561 cggcggactt cctcgatcat ggcgaaggcg gagatcccga tggcgtccat ggccaggctg
     1621 gagaagagga aggggatcat ccccccgatc aggaacccga ccagcaccgg cacgctcacg
     1681 atggagaggt ccagggtttc cgccgccccg gtggcctgga cgaaggcgct gaagaggatg
     1741 atggaggtga gggccgcgga tcctaccgcg aagcccttgc cgatggcggc ggtggtgttg
     1801 cccaccgcgt ccagggaatc cgtgatctcc cggacctcct tcttcagggc gctcatgacg
     1861 gccaggcccc cggcgttgtc cgcgatgggg ccgtaggcgt ccacggagag ctggatcccc
     1921 agggtcagga gcatccccag ggccgcgatg cccactccgt agagtcccgc cagctggaag
     1981 gagcccagga tggccgcggc gatcacgagg gtcgggggga gggtggagag catgccgact
     2041 cccaggccgc tgatgatggc cgtggcggat cccgtgtcgc aggcccgggc gatcttgagc
     2101 accggcttgg tcgtggttcc cgtgaagaac tcggtcaggg tcccgatgcc gacccccgag
     2161 gccaggccca ggaccgcggc caggaaaatg tgcagggcgc cgtacccctt ctcgaaggtc
     2221 gagtccccca ggatgagggc gatgaccggg tagagcaggg cggtggcgag gatcgcggcc
     2281 ccgaaggtcc cggtgttcag ggccttctgg ggggacttgc cgggcttggc gcggaccagg
     2341 aaggttcccg ccacggaggc caggatcccg atcgcgcaga gggccagggg caggaaggag
     2401 agctgcatct tgaccgtatc cgtggcgttc atcacgttgg cgcccaggac cacgcagccc
     2461 acgatggagc cgacgtagga ctcgaagagg tcggcgccca tgccggccac gtcgcccacg
     2521 ttgtccccga cgttgtccgc gatgaccgcg gggttccggg gatcgtcctc ggggatgccc
     2581 gcctccacct tgcccaccag gtccgccccg acgtccgcgg ccttggtgaa gatcccgccg
     2641 cccacccggg cgaagagggc tatcgtgctg gcccccaggg agaagcccga gaggatgggg
     2701 aagaccacgt cgtggagggc gccggcgctc tcgccgaaga gccggaggcc cgcggccagg
     2761 accgccgaga tcccgagcat ggccaggccg accacggtca tgcccatgac ggaacccccc
     2821 gcgaaggaaa cccgcagggc tccgtcgatc cccgtctccg ccgcctgggc ggtccgggaa
     2881 ttggcggcgg tggcgacctt catgccgaac cagccggccg ccgcggagca gagggcgccc
     2941 agggcgaagg acagggcctg gaggcgcagg ggaccgctgt tggccaccgc caggaagagg
     3001 gcggcgatcg cgacgaaggg taccaggacc ttgtactccc gggttaggaa ggcctcggcg
     3061 ccctcggcga tgtatcccga tatgcggacc agggtcgcgt taccgacctt gatacggtag
     3121 atccagcgag ccttgaggaa ggcgtacagc accgcgagtc cgcctccgac ggcggcgacc
     3181 gcgatcaaca gggagggcat ggcttctctc cttcgtcgtt ggtgtatcgc cccggagtcg
     3241 acttccccgg gcgcgggggt agtataccgt ggactgtgga cggtggacag tggaaagtga
     3301 acgggatagg agaggtaccg gaggcttgaa ataggaccct cgaaggggta agttataatt
     3361 agctaactat tcagggaggt atgtcccatg accatacgag tcgtgtactg gagcggtacg
     3421 ggaaacacca aggccatggc ggaagccgtg gccgagggtg cccgatccgc gggggccgag
     3481 gcggaactgt ccgaagtcgg gtccgcggac gtccggtccg tactggcggc cgacgcggct
     3541 gccctgggct gcccctccat gggggccgag accctcgaag aggaggagat ggagcccttc
     3601 gtgcgaagcc tcgaatccgc aggactcgag ggcaagccgg tcctcctgtt cggctcctac
     3661 gactgggggg acgggcagtg gatgcgggat tgggaagagc gcatgaggaa ggccggcgca
     3721 gccctggcgg gcggcgggtt catcgtgaac ctgacgccct ccgcctcgga cattgtggcc
     3781 ctggcggagg cgggaaggaa cctggccgca gcggtttccg ggaagtaact gaacccgtag
     3841 ccccgcccgc cgccgcgcgt ctccgggcga ctacgcgccc gcaggcgctt tacgtccgct
     3901 gtccaccggc cgccgacccc gtacttcccc cgcccgggca tgggcttcca gcccttccat
     3961 ccgtgcgaag gcggcggcgt cccggtagag ctccgaagcg tcggcggggt cgtcgatccg
     4021 gatccaggtc cgtacccgca ggaaggacag ggccgagagg cccccggcca ggcgggcgtg
     4081 ggcgcccgtg ggaaggacgt ggttgggacc ggtcccgtag tcccccagga cctccgcggt
     4141 cccggcgccc aggaagacgg cgccggcgtt gcggatcttc cgggccaggg cctccggttc
     4201 ctcgcacagg atctccaggt gctccggggc gaaggcgtcc gccgcgcgga caagagtctt
     4261 caggtccggg tccaccagga cccccccgtt gtccagggca cgtcgggcga cctccccggt
     4321 cgggagatcc gccaggcggc gctccagggc ttcctccacc cgggcggcga aggcctcggt
     4381 acggcaggca aggacgggga ccgcatcggg gtcgtgttcc gcctgggcca ggaggtccgc
     4441 ggcggcgatc tcgggatcgg ccccgtcgtc cgccaggacc aggagctcgc tggggcccgc
     4501 cacggactcg atgaccgtgt cccccgccac caggcgcttg gccgcggcca cccagcggtt
     4561 gccggggccc accacccggg cgcagggagg aaccggcccc gccccgtagg ccagggcggc
     4621 gatcgcctgg gcacccccgg cggccaaaag gccgtccgcc ccggccacgg cgcaggccgc
     4681 cagggtttcc cgggcgggcc ggggactcgc ggtccagact gtcccgaccc ctgcggcctt
     4741 ggcggtcagg gcggtcatga ggacgctgga gggcagggga taccggcccc ccggggcgta
     4801 gcagcccgcg gtctccacgg gcaggacctc gtggcccgcg aagcccccgg gcacgggtac
     4861 ctccagggcc gagaggctgg cggcttgtgc cttggcgaag gccccgatcc gggcccccgc
     4921 ccgctccagg agttcccggg aatcccgggg cagggcgtcc aatgccttcc ggaagtcccg
     4981 gggaccgagg tacaggggcc cctcccccag gccgtccagt tcccgggcgt acccgaggag
     5041 ggccgcctcc ccgcgctccc gaacgtccgc cacgatggcc gccgcccggt ccgccgtcac
     5101 ggggtcgggg atggaggcgg gtttccgttc caggtccgca gcacggaggc gccggagcct
     5161 cagtggattc cggtccaccg ggtgcctccc tcctcctgga acgccggctt ggcgttcccc
     5221 gggcgccgca caaccttcag ggcccgccgg tccaggatgc gttccacatc cgccagggac
     5281 gccccctcgg cccggagctt caccagggcg aagtagacga ggtccgcggc ttcctcggcg
     5341 gcccggacgg gtccctccgc ctccgcgagt tccgcggcct cctcccggat cttggcggac
     5401 aggaggcccg gttccgtgaa aagccggtgg gtgtaggaac ccggggggga ggattcctgg
     5461 cggccccgga tggtccggtc cagggccccc aggccttggt cggggccgaa gcaggtgcgg
     5521 gtgcccaggt gacagaaccc gccgccgttc tggcggacca cgtagcggag ggcgtcccgg
     5581 tcgcagtcga cctcgatgcg gaccaggtcc tgggtgttcc ccgaggaggc acccttttcc
     5641 cagagccccc gagtccggga ccggtagacc ccccggccgc gcttcaggga ttccgccacg
     5701 ctctcccggt cgccccacac gaggcccagg gcgatcccgg cttcgtcgca gaccaccacg
     5761 ggccagagcc cgtcggggcg gtccgaagcc aggggggccg tgaaggcttc cgccagggag
     5821 agcctgccgg agtacagggc catcccgacc tgggcgtccg cccccaggcg gtccagttcg
     5881 gccacttcct cggccgacgt gacgcctccg gcccaggtga tccggaggtc ctcccggcat
     5941 tcccgggcta tgcggacgac gtccgccgcc gcggccaggt ccgccccggc catccgtccc
     6001 tcccgttcca cggtggtcac caggaagccc ccgacgtagg gggccaactc ccggatccgg
     6061 tccgccacgg aggcccccgt gcgggttcgc caaccctcca ccacaacttc cccgttccgg
     6121 gcgtccaggg cggcgatcac ccggtcccgg ggcaggcgcg aaaggaggtc cggccgggcc
     6181 gcggtcccca ggataacctt ccgggccccc gcgtccagga ggcggaccgc ggtctcgtag
     6241 tcccggatgc ccccgcnnnn nnnnnncacc cggcagggat aggccgagac gaggtcctcc
     6301 accaggcggc ggttgtcccc ctccccccgg gccgcgtcca ggtcgatcac ggcgagctcc
     6361 ccgacccggg agaactccgc ggccttgggc cgggggtccc cggcgtcgat cctgagggtc
     6421 tctccgccct cgagctggac ggcccggccg cccatcaggt ctatggaagg aacgatcatg
     6481 gtcttacctc cacctgtctt tcggccaagt atgccttgac ctcgtgtatc gtgcggcgcc
     6541 cgaaatggaa ggtcccggcc gccaggaccg cggccgcccc ggccgcgaca ccctcgtaca
     6601 tctgccgggg ggtttccgct cccccggagg ccaccacggg cacgggcacc gcccgggcca
     6661 cctcggacac caggtccagg tcgtagccca gccccgtgcc gtcccggtcc cgtccggtca
     6721 ggaggacctc cccggccccc gcctcgacgg cccggcgggc ccaggccgcg gggtccaggc
     6781 ccgtccgggt ccgccccgcg tccaccgtca cctcccaggg ggaggcgcct tccggcctgg
     6841 cgccggacag gcgtccggcc gcgtccggtc gccttgcggc gtccggtcgc ctcgcggcgt
     6901 ccacggcgat caccaccgcc tggaccccga agcgctccgc cagttccgtc aggatggcgg
     6961 gatcccggac ggccttggag ttcacggata ccttgtccgc ccccgcctcg aagaggaccg
     7021 ccgcgtccgc cacggatccg atccctcccc ccacggtcag ggggatgccc agggcggccc
     7081 ggacccggcg gaccgtgtcc gcccgggtgg cccggccctc gagccccgcg gtgatgtcca
     7141 atagcacgat ctcgtccgcc ccctcgccct cgtaccgggc ggccagctcg gcgggatccc
     7201 ccgcgtcctt gaggtccagg aaccgggtgc ccttgaccac gcggcccgcg tccacgtcca
     7261 ggcaggggat gacccggacg gaccgggtgc ccgacagggg ctcggcgggg gggcgggata
     7321 cccgggcccc ggggccctcg gccgtcaatc cgtccgccgc cccggctccc tcgagccacc
     7381 gggaaaggag ggccttcccc caggccccgg agagctccgg gtggaactgg cagaggagga
     7441 ggcccggcct ctccagggcg gccgcgaagg ggcccgcgta atccgcccag gcggtgcgca
     7501 ggcccggggg cggctcgacc aggcggtagg agttggcgaa ggccgcccac ccgggctcca
     7561 ggcagcccga gccggggtcg ggaaggacgg ccccccagcc cagccggggg gtgcgcacgg
     7621 tgtcggggta cttccggatc gtcccgggaa ggagggccag gccccggacc ccggggctct
     7681 cctcgctggc ctcgaaaaag agctgcatgc ccaggcagaa ccccgccagg ggcctgccgg
     7741 cccggaagcg ctccgccagg gcccggtcca ggccccggga gcggagctcg gccagggccg
     7801 ccccgaagga gcccaccccg ggcaggatca cccggccctc ccgcaggaca tccccgggat
     7861 cctcggtgac gtagggctcg gccccggccc tccggagccc cgccaggacg gaggccaggt
     7921 tggcggtgcc ggtgcggacg acggccgtcc tcatgccagg acccccttgg cgctgggcac
     7981 cgcgccggag cccgagaggg cccaggcctc ccggagggcc agggccaggg ctttgaaggc
     8041 cgcctcggcc ttgtggtggt cgttgtcccc gtacaggacc tccaggtgga ccgtgatccc
     8101 cgcccgggtc gcgaagctcg cgacgacgtg ggggatgttc tcgcaggcca gggtcccgac
     8161 ggagtcccgg gccagcttca ggtcgacacg ggcgaatggc cggccggaga tgtccaccac
     8221 cgcccgggcc agggcctcgt ccaggggggc gtaggcggag gcgaagcggc ggatccccga
     8281 gcggtccccc agggcctggc gcacgaggtc ccccagcacg aggccgcagt cctccgccgt
     8341 gtggtggtcg tccacggcca ggtccccccg gcaggccagg gccagggaga atcccccgtg
     8401 gaaggccagg gacgagagga ggtggtccag gaagccgatg cccgtgtcca ccttcacctc
     8461 cccgggcgcg aggtccaggg aggcctcgat ccgggtttcc cgggtctccc gacgcaggga
     8521 cgccctccga agggccggaa cggagatcgt gtcgctcata ggtactcctt cgattcccgc
     8581 agggcccggg ccaggactcc gaagccctcc gggtccgcgg ggccgctgat gcggatcatc
     8641 gcctcgaggc ccggacgccc cggataggcc cggatggcga cgccccgaga ggccagggct
     8701 tccttgaggc ccgagggatc cttcacccgg gccaggacga agttcgtctc cgacggatag
     8761 ggccgggcac cgaggtcccc gagcaggcgg tacagggtct cccgttcccg gaccagccgg
     8821 gtcacgatgc gggaagtctc cgcctccccc tcggggctcc gggcggccag ggccgcggcc
     8881 agggaggggc ccgccacggg aaagggatcc cccgcggcgc gcagggccgc gatggtctcc
     8941 ggagtgcccg cggcccagcc cacccgcagg cccgcgagtc cgtaggtctt ggagaaggac
     9001 cgcagggcca ggccgttggg ccgggacagg gccgcggcca gggaatcggt ccgcgagaag
     9061 tccgcgtagg ccccgtccac ccagacgctc gtatccggga gggcgtccat cagctcctct
     9121 gttacctccg gggaggccag tccccccgcg gggttggcgg gattgaccag ggccacagcg
     9181 gcggcccggc aggatcgggc cgtctcgatc gcggcttcca gggggaaggg ctcccccggg
     9241 ggccagggca ccccgacggt ccgggcgccc gaaaggcggg cgccgatgga gtacatttcg
     9301 aaggccggtt cgaagaccac cagggtgtcc ccgggcttga ggaagacccg gcaggcccgg
     9361 gcgatggcat cgtcccctcc cgcggtcacc aggacccggg agggggaaag gggctcgtac
     9421 cccgccttgg gccgggccgc tatgaccgcc tccagttccg cggtgcgggg gtagcggcgg
     9481 gaggtttcca tcgtcgccgc tcccgccagg gcggccaggg cggcccggga ggggcggccc
     9541 tcgttggcgt ccaggaacca ccggatccgg gggtcccggg cgggagggac gtagcctacg
     9601 accggccgtt cctgggcccg gtacccgtcc cgctcgtccg cggtattcac ggcacgatcc
     9661 tcatgggctg ggtgacgatg atgtccgtcc cgccccgcct ccggatctcc gggatcaggg
     9721 ccgccaggga gtcccggggc gccgccacct tgaccgcgta cccgtcccca ccccggaggg
     9781 gggagaccgt aggctcccgc atgcagggca ggatggcgat gaccgagtcc agcttgtagg
     9841 tgtccacgtt cagctccagc atgacccggc tgcgggcggt caggacgctc cgcaaaagga
     9901 ggacgatggc ctcgatggtg tcccgcttcc aggggtcctc cagggaccgg gggttggcgt
     9961 agaggcgggt cgagctccgc aggagctcgt ccacgatctc gaggccgttg gcccggaggg
    10021 tcgagcccgt ggcggtgttg tccacgatgc agtccgcgtc gtccgggggg aagacctcgg
    10081 tggccccgta ggaacggacg aatgtggcgt ccaggtcccg gccggcgatc cagcggcggg
    10141 cgatgttctc gtactcgctg gccaccacca gggggcggcg gggcaggcgg ccgtcctcca
    10201 ggatggccac cggggccgcg gccaccaggc gcacggggtc cagccccgtg tccagcatgt
    10261 ccgtcagctc gacccccagc tcttccaccc agtcggagcc cgcgaatccc aggtcccggg
    10321 tgcccgcgtg gagcatctcc agggtgttcc ggggtttcag gatcttggcg tccaggtccg
    10381 gggaggggag gcggggccgg tagtcccgct cgtccccctt gatgggcagg cccgcgtccg
    10441 aaaggaggcg gaacacccct tcctgcatgc gccccttggg cagggcgatc ttcaagacct
    10501 tcgcttcgtc catatccgtc ctctcgcgtc gaaaaaaacg ggggcgccgg tccactacgg
    10561 ccggcgcccc taaaaataaa ggcgccgacc tcgcggtcgg cgccccgtcg ttcggttcga
    10621 tgcacctctc ggtacgatcc tacctcccgc cgtgccgcga ccgcgacccg tggtgcccgt
    10681 gatgatggag gcgattgatg tttcccgtac ccagggtaat catactttgc atagaaacac
    10741 actcgcggcg tacctgtcaa gaggtacctc ccgcgccggc agtacgaggc ttgtgaagat
    10801 cctccggacc tcctccttgc tccgtccccg gctacggagt tagtctatat ccatgttcac
    10861 tcccctggag ctgatctacc ccgtggtctc ccgccgagcc gggggcctgt ccctgggggt
    10921 ggatctcttt ccggaccgca agacctgcaa cttcgactgt ccctactgcg agattttccc
    10981 gtttcccggg gagcccccct tccggccgga ggatctcgac agggagctcg ctgagttctt
    11041 cgaggtggac tacccctcct tccccccgga ccttcccctc cgggacctca ccctgagcgg
    11101 gagcggggag cccaccctgt cgccccacct gggatccgcc ctggcctcga tccggaccgc
    11161 ccgggaccgc tgggctcccg gggcggatct ggtgttgatc accaattcca ccaccctggg
    11221 gcatccggaa accgcggccc tcctggggcg ctgcgtggac gaggcgggcc tccgtatctg
    11281 ggccaagctg gacgggggga acgaggagtg gtacggccgg atgagccgca gccgcgtccc
    11341 cttccgggat gtcgtggagg ggatcctgtc cttttcctcg gcccatgccg tgatcgtcca
    11401 gaccatgctc tgcaccctgg acggccgggg accctcggac gcggacctcg ccgactacgc
    11461 cgggctggtg gaggacgtcc tcggccgggg ggcccggttc ctggagatcc acctctatac
    11521 ccaggcccgt ccctccccgg agggccggac ctcccccctg ccggaagccg atctttcggc
    11581 cgccgcggac ctcgtgtccc gccgcgcccc gggggttccg gtccgggtct tcggccgcac
    11641 cggggagctt tcacggtgat cgacctgcac acccactcca ccgcctccga cgggacctac
    11701 gatcccgagg ccctggcaga tctggccgcg gaccgcaagc tcacggccct ggccctcacg
    11761 gaccacgact ccctctcggg cctggcggag gcccgggcgg aagccctgcg gaggggcctc
    11821 cgcttcatcc cgggggtgga gatcgaggtg gccttctcac ccggggagtt ccacctcctg
    11881 ggcctggacc tccgggatac cgagggggcc atccgggagg cggtgggacg cctggcccgg
    11941 tcccgggatg agcgcaacga gcggatcttc gagcggatgc gggccgcggg gatcgaggga
    12001 gactacgggg aactccggga ggccgccgcg gggggcatga tcggccggcc ccacatcgcg
    12061 gatttcctga tcgcccggaa ggtggtccgt aagaagcagg acgccttcga caagtacctg
    12121 ggcaagggcc ggcccttcta cgtggccaag gaatgcctgg aactgaagga ggcggtccgg
    12181 gtgatctacg aggccggggg cctgcccatc gtggcccatc ccctgtccct cttcgtgtcc
    12241 tggtcccgcc tggggtccct cttcctggaa tggaaggagc tggggatcgc ggggatcgag
    12301 gcctggcacc cgaccgcccg gccggggcac tgccagcgcc tggagaagat ggcccgggac
    12361 ctgggcttcc gggtgaccgc gggcagcgac ttccacggcg ccaaccggcc ggaccggcgc
    12421 ctgggctaca cggcggggga ccggcctatc gcggactcgt acctggaggc cctggaccgt
    12481 tgactccggg aggctcgggg gccgcctcgg tttccccctc cccggcgtcc gggcccgtcc
    12541 cggcatccgc atccccgtcg acccccgccg gggaccccgc ggtgtcggat tcccctccgc
    12601 cgcgcccgga cttccgggtc ccgtaggcct gctcgaagag gtccaccccg gtgaagaaga
    12661 gcatcagcat catgggcccc aggatgaggc cgttcagacc gaagagctgg aggcccccca
    12721 tgatggagaa gaagaccagc agggggtgga tcttgatgcg ctccgtgagc aggaggggcc
    12781 gtagaaggtt gtccaccccg gaaaccagga tgaaggagag gatcaggatg gcgaatccgg
    12841 ccgtcggatc cccggatacg atgcgcgcga ccccgacggg tccccagacc agggtggtcc
    12901 ccaccatggg cacgaaggag gctatggccg tcaagaaccc caggggcagg gggcccttca
    12961 cgccgaagag caggaatatg aggaaggccg cggttccctg gtagagggcc acgagcaggt
    13021 agccccggaa gaggtgttta ccgacctccc cgaaccttcg caggaaggcg gcggtgtagg
    13081 cgttctcgat gggcacggcg ttcacgaagg tacggatcag atccttgccg tccacgtaga
    13141 agaagtagag ggtgaaggtc aggaaggcca gggagacgcc gaaccccgcg gtgtcccgga
    13201 ggagggtcgc cgagacggtc aggaaggtct ccgcaccgcg ggtgaccgcc gcggacagtt
    13261 ccgacatcag gtccatcccg gtcaggtcta tgaggcctcc ggaggtgtcc cgcaggcgca
    13321 tggccaggcc gcccgtgtcc agggtccgga agaagtccgg attccgttcc aggaactcgt
    13381 cgatcgagtg gacgagctcc cgggcctggc ccagcaacgt ggagcccaga aaaaccaggg
    13441 ggatgaccgc ggccaggacg gagcccaggg cgaagcctcc cgccagcagc ttgcgcgcga
    13501 agcgtcccag gggcttgtcg gggatcgagc gggtcgcccg gtcgtagaag ggaaaggtta
    13561 tgacgtacag gagtcccgcc cagagcagga tgctgaagaa gggggagaac agccgcgcca
    13621 cgaacaggaa caggaagagg aacaccgcca ggaagaggag tctctgtact tgcttgccct
    13681 tcatagtatc gccttcgtcc gtcttccgga tttcacgtgt tccggcagcc cgagcacccc
    13741 aggacgtgga cgtgcttgcg gccgggatgc tcgaagagcc gggcgatcac gtagccctgc
    13801 cgggggactt ccttctcgca gaccggacag atccgcttgc attcaatgtt gggggggtag
    13861 cagtggggac agccgaagat ctgcatgagc ttgtcccctt ggcccgggtt cacccgggtc
    13921 ttgaccctct gccccggcga caggacctcg ccgcagaggg ggcaggtccg gggcaggtcc
    13981 tccgtcgcgg ccttgtcccc cgccttgagg agggtccggc gccgccggta cgggccgaag
    14041 aggcccagga acatccacag caggatgacg gcgaccgaga tgtagatggt ggcgcgcagg
    14101 aggatgtcca tcgggggaag tatatcgtgg aaggtgaata ggtgaatagg tgaatgggtg
    14161 aatgggtgaa tgggtgaatg ggtgaatggg tgaat
//
LOCUS       DAOTEI010000086        14182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2964_length_14182_cov_5.674453, whole genome shotgun sequence.
ACCESSION   DAOTEI010000086 DAOTEI010000000
VERSION     DAOTEI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_14182_cov_5.674453"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="P5313_09470"
     CDS             complement(<1..1393)
                     /locus_tag="P5313_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRY80634.1"
                     /translation="MSSPEGSGPAPRFGLAALVASFALVAGGSFPLARAALLSGAPRA
                     GTPTLVYAALLAVVPLSLLGILAFRIRGLVADYRVRGYGSRLRAKLSRQFLLIALLAS
                     VPHGVFLGALLRTAVRAPWTATTQDALDAGFQVAVAFQEERLARLAYLTERDAVVLGE
                     RLGWDASVLLAALAERESGAAAVEVFRDGLSAGFAGAETARLGDVRAASPGPLSRVGA
                     GDTVYLRHLARAPGSGARSVAVSLRLPPEIDRAAAALGRVRRMEAVPGFMDRRFAPFL
                     AFYWFLFALPPLAIALLAGLSAADTIVRPVAALEEAALAAARGDLTPRLLPRPGDELG
                     GLVSAWNRMLSGVEAARYEAAAAEKVAAWRDIAQRLAHELKNPLTPIRLSAERVLRKW
                     RSDPASAGEILEPSMLAILQETQVMNSLLSDFRSFASLPEPAPDWTNLRALVEEACSP
                     YRASFPEARFDAEN"
     gene            complement(1390..1656)
                     /locus_tag="P5313_09475"
     CDS             complement(1390..1656)
                     /locus_tag="P5313_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRY80635.1"
                     /translation="MTEKSVSIKNRAGIHARPAALIVQTASKFKSKIFIEKNSDRINA
                     KSIMGIITLGAGFGVELRVLAEGEDEAQAVEAVALLFDRKFEEE"
     gene            complement(1679..2668)
                     /gene="hprK"
                     /locus_tag="P5313_09480"
     CDS             complement(1679..2668)
                     /gene="hprK"
                     /locus_tag="P5313_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRY80636.1"
                     /translation="MDTVRFSVLDLLNLDLREHNALNLRCIGGRKGLGRQISVPDLNR
                     PGLALSGFFDAFAFQRIQVFGRGEYAYLEKLEREGSLGVAKRVFEFEMPCCIFTHSLA
                     PQAEFLASAEAANCPILQTDLSSSEFVGRILRVLSDIFAPKTSIHGVLVEVFGIGILL
                     SGDSGVGKSETALELIERGHRLVADDVVEIRCVNGNYLMGQGANKIVGHHMEIRGLGI
                     INVTHLFGVGAIRDKKQIQLVCNLEEWDADKVYDRIGTDEKTVDILGVMVPQLDIPVK
                     PGRNIPIIIETAAMNERLKKMGYHSAKEFNRNILRWLESENARAVYFGQDDIL"
     gene            complement(2825..3118)
                     /gene="raiA"
                     /locus_tag="P5313_09485"
     CDS             complement(2825..3118)
                     /gene="raiA"
                     /locus_tag="P5313_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRY80637.1"
                     /translation="MNVDVRSVHFDLGAPTRDYLDKKLERLNFAKDTIVTVDFAFTKE
                     KDFICESTIHFRWGHQAHIQERDFELAAGIDKLMDKMEMKVRKEKEKVQDRNK"
     gene            complement(3207..4580)
                     /gene="rpoN"
                     /locus_tag="P5313_09490"
     CDS             complement(3207..4580)
                     /gene="rpoN"
                     /locus_tag="P5313_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRY80638.1"
                     /translation="MQLQKPVLVQEQRQKLSPQMIQSIRLMALPLPELKDEIQQEIEA
                     NPALEVLEDRSTVSLESFPEERESDSDAARVYENASDPGYGRGRDPNDDSKRMFLEGA
                     ISRAETLQEHLLWQLRLQPVPEKVRAAAENLIQNLDSDGFHKEDPRLVCRDTPEDAFK
                     SALSLVRGFEPVGVCASDYRESLIVQAELCPEAPPRTVEVLRDHIELLEKGKHEDIRK
                     RLKLSEADLSSILAFIKTLNPFPGRQYSSSEPQYVIPDIQVKLKDGEFVIVLNDEEIP
                     VLGINPFFDRLSEEKHDRTTDVFVKENIRRAKWFIQSIHQRNKTLLKATRAIVEFQRS
                     FFTKGPKHLAPLTLRDIAGEVGVHETTISRLANRKYVQTDWGIFELKYFFTNSISGAG
                     SSGSRFSKEGVKQVIKEIIENEARALSDRDIADQLARKGINLARRTVAKYRLELNMDS
                     SFGRRHE"
     gene            4797..5459
                     /locus_tag="P5313_09495"
     CDS             4797..5459
                     /locus_tag="P5313_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRY80639.1"
                     /translation="MSKTSKTESKTESKTTAPEQASGTGPLPEDACEATEDLARILEA
                     RMAEAQKRAAELQAENSELKDQYLRKLADYENFRKRMFREKDDAVQFANSALLHDLLG
                     ILDDFDRAVSSSEVSRDFQVLHDGIVMVRNRMLSTLEGKYGLARFDSAGASFDPNVHE
                     AVAMEKGPVPDPVVAEEFQKGYKLHGRVLRAARVKVRMPDGSAPEAPAPDAPPPSAGE
                     TK"
     gene            5505..7442
                     /gene="dnaK"
                     /locus_tag="P5313_09500"
     CDS             5505..7442
                     /gene="dnaK"
                     /locus_tag="P5313_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRY80640.1"
                     /translation="MGRIIGIDLGTTNSCVAVMEGGEPLVIANSEGQRTTPSIVGFTG
                     KGERVVGQPAKNQMITNPENTVYSIKRFMGRRLEEVQTEIKLVPYTVVSHQNDVRVAV
                     SEKQYSPQEISAFVLQKMKKTAEDYLGETVTEAVITVPAYFNDAQRQATKDAGKIAGL
                     EVKRIINEPTAAALAYGFNKDQKKEKVIAVYDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDRRIMQWLIDEFKKDTGIDLSQDKMALQRLKEASEKVKIELSNVQQAEVN
                     LPFITADASGPKHLQKSLTRANFERLIEDLLEKSKDPCRKALQDAGITAAQIDEVILV
                     GGSTRIPKVQTIVRELFGKEPSKGVNPDEAVAVGAAIQGGILGGDVKDVLLLDVTPLS
                     LGIETLGGVFTRLITRNTTIPCRKSQIFSTASDGQTAVSIHVLQGEREMAGQNRTLGR
                     FDLVGIPAAPRGVPQIEVTFDIDANGIVHVSAKDLGTGKEQKIRIEASSGLNDDEINK
                     MVKEAEMHAEEDKKERERAEVRNEADTLAYATEKSLKEYGEKVTASDRESIEKAIKEL
                     REAVGSNDTARIREKIEVLKQASYKLAEEIYKNVGQGGQAGGDGQGPQGSGPQGQPEP
                     ESGPKTAEDVDYRVVDEDEKK"
     gene            7513..8658
                     /gene="dnaJ"
                     /locus_tag="P5313_09505"
     CDS             7513..8658
                     /gene="dnaJ"
                     /locus_tag="P5313_09505"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRY80641.1"
                     /translation="MAKRDYYEVLGVPKDSSKDDIKKAYRKMAIANHPDKNPGNKAAE
                     ERFKEATEAYEILGDDQKRAAYDQFGFAGVEGMGGGAGGQHDFSSVFRDFEDIFGFGD
                     FSNIFDSFFGGGRAGGARGRSGVGKGQDLRYDLELTFEQAVFGTAVEISYARNDSCPT
                     CKGTGASSGGGRKVCPQCQGSGQVRRSSGFFSIASPCPACGGDGYIVENPCRDCAGTG
                     LAKKRQKIKVTIPAGVDDGKRVQIPGQGDTGANGGPPGDLYVFIHVRPHEFFERDGYD
                     LYCAVPVTIAQAALGAEITVPTIEGKKISIAVPAGVPHGKVLRIREEGVPHASSNSRR
                     GDMYVKVMIQVPAKLSKRGRELLEEFARIENDPTDPRPLRLSELKNN"
     gene            complement(8676..8963)
                     /locus_tag="P5313_09510"
     CDS             complement(8676..8963)
                     /locus_tag="P5313_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRY80642.1"
                     /translation="MPDTSPELKSAFECRSGCGACCVAPSIAPTLPALPEGKPAGVPC
                     PHLTVDFRCALWGRPERPAFCGSLRADPEMCGESREYALAWLADLEEKTRP"
     gene            9055..9137
                     /locus_tag="P5313_09515"
     tRNA            9055..9137
                     /locus_tag="P5313_09515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9088..9090,aa:Leu,seq:caa)
     gene            complement(9293..9862)
                     /locus_tag="P5313_09520"
     CDS             complement(9293..9862)
                     /locus_tag="P5313_09520"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRY80643.1"
                     /translation="MAPKRCPWAGTDPDYVRYHDEEWGMPLRDDRALFELLVLEGSQA
                     GLSWITILKRREGYRRAFEGFDPERIARYGPADEARLLADPGIVRNRLKVGSAVRNAR
                     AFLALREERGSFSDYLWGFTEGRSVVNRWKSMSEVPARTDLSDRISEDLKRRGFNFVG
                     SVIIYAFLQSAGLVNDHLVSCFRWKECQG"
     gene            10069..10464
                     /locus_tag="P5313_09525"
     CDS             10069..10464
                     /locus_tag="P5313_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRY80644.1"
                     /translation="MPNLVPGLTGERTLIVEERHTAAHLGSGGVPVYATPMMILAMEE
                     AALSAVDPRLEPGQVTVGYHLDVKHLAATPLGMRVTARAELLAVDGRMLSFKVEAFDE
                     KEKIGEGTHVRAIINVAKFKEKLAAKSGV"
     gene            10667..11764
                     /locus_tag="P5313_09530"
     CDS             10667..11764
                     /locus_tag="P5313_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY80645.1"
                     /translation="MKNRWNLLWILLAAALIAGCAKKSEPAAVAAKAEPYKVAFVYIG
                     PPGDLGWTHEHDQGRKAVEAEFGDKVKTVYIENVPEGPDATRIIRQYAQEGYDMIFAT
                     SFGYMDPMVEVAKEFPKVKFEHCSGYKTAENMSTYFGRIYQPRYLSGIVAGKMTKSNI
                     IGYAAAFPIPEVVRGINAFTLGARSVNPKVQVRVVWTNTWYDPVKEREAAVALLDAGA
                     DIIAQHQDTVEPQKAAKERGKLSIGYDSDMRAFVGDTVLTSPVWNWGSYYKDTIGKAM
                     AGTWTTHQYWEGLKAGVVRLADMSPLVPQDVKDLVKAAEQKILDGTWDVFYGPIKDNT
                     GAVKVAAGAKMTDGEMLSFDWFVEGVVGKVK"
     gene            11913..13451
                     /locus_tag="P5313_09535"
     CDS             11913..13451
                     /locus_tag="P5313_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80646.1"
                     /translation="MDASEPKVPLVEMRGIRKSFPGVTACDGVDLRLGRGEVLALLGE
                     NGAGKSTLMNVLAGLYRPDEGEIRIGGKPVEIRSPRDSAAHGVGMVHQNFMLVDTMTV
                     AENVVLGMAGLPLVPDMAEVKRSVLELAEKYGLRVDPDAYVWQLSVGEQQRVEILKQL
                     YRKAEILVLDEPTAVLTPQESEELNVVVQRMKAEGKSAVFITHKMEEVMTFSDRVMVL
                     RKGKVVVEKVTSETSPRDLAKLMVGREVLFRVEKKSFEPGAVILELKDVRALDDRGLP
                     ALRDLSLKVRAGEILGIAGVAGNGQRELSEVITGLRPVSSGGISINCVETTNKTPLEI
                     IRSGVSHIPQDRIAVGAVGDMSVASNLAMKGYRDAPISRGVLLLPRRILDLARRMIES
                     FRIATPSPETRVKFLSGGNIQKTILAREIESCRSLLVAVYPSRGLDIGATEAVRKELV
                     AQRDAGMAVLLVSEDIEELLSVADTVLVLYEGRAMGTFPSAEADPEELGLLMAGVERK
                     ETVS"
     gene            13448..>14182
                     /locus_tag="P5313_09540"
     CDS             13448..>14182
                     /locus_tag="P5313_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY80647.1"
                     /translation="MSILFEKRKNISRTAQVLVPAASFVVSLILTGILLLAFGADPFA
                     TYRAMFRGAFGSGRAFTETLVKAIPLMLTGLGVALAFRLRFWNIGAEGQLTLAGVAAT
                     GVAIFWGRWMPSFLLLPTVILVGCLAGAAWAGIPALLRTRLKVDETLVTLMLNYVAIL
                     FSEYLYYGPWRDPKGYGFPGTEPFPEAAWMPRIAGRAHLGLVFALFLALALWFVLNRT
                     RWGFELKILGASPQAARYQGISVERNI"
BASE COUNT         2555 a         4657 c         4763 g         2207 t
ORIGIN      
        1 cgttctcggc gtcgaacctg gcctccggga aggaggcgcg gtagggggag caggcttcct
       61 ccaccagggc acggagattg gtccagtccg gcgcgggttc cggcaggctg gcgaaggacc
      121 ggaagtccga gagcagggag ttcatgacct gggtttcctg gaggatcgcg agcatggagg
      181 gctccaggat ctcccccgcc gaggccgggt ccgaccgcca ctttcgcagg acacgctccg
      241 cggacaggcg gatgggggtc aggggattct taagctcgtg ggccaggcgc tgggcgatat
      301 ccctccaggc ggccaccttc tccgccgccg cggcctcgta ccgggccgcc tccacccccg
      361 agagcatgcg gttccacgcg gacaccaggc cccccaactc gtcccccggc cgggggagga
      421 ggcggggagt aagatccccc cgggcggcgg ccagggccgc ctcctccagg gccgccacgg
      481 gccggacgat ggtatccgcg gcggaaagcc ccgcgagcag ggcgatcgcc aggggcggga
      541 gggcgaagag gaaccagtag aaggccagga agggggcgaa gcggcggtcc atgaagcccg
      601 gaaccgcctc catccgccgg acgcggccca gggcggcggc ggcccggtcg atctcggggg
      661 gaagtcggag gctcaccgcc acggacctcg ccccggatcc cggggcccgg gccaggtggc
      721 gcaggtagac ggtgtccccc gcgcctacgc gggacagggg acccggggag gcggcccgga
      781 cgtcccccag ccgcgccgtc tcggcccccg cgaagcccgc ggaaagcccg tcccggaaga
      841 cctccaccgc cgcggccccg gactccctct cggccagggc ggccaggagg acggaggcgt
      901 cccatccgag ccgctccccc aggaccacgg cgtcccgctc cgtcaggtag gccagccggg
      961 ccaggcgctc ctcctggaag gcgaccgcca cctggaagcc cgcgtccagg gcgtcctggg
     1021 tggtggccgt ccagggggcc cgcacggccg ttcgcaggag ggctcccagg aagacaccgt
     1081 gggggaccga ggccaggagg gctatcagga ggaactggcg ggaaagcttg gcccggagcc
     1141 ggcttccgta gccccggacc cggtaatccg cgacaaggcc ccggatccgg aaggccagga
     1201 ttcccaggag ggaaagggga acgacggcca gcaaggcggc gtaaaccagg gtcggggttc
     1261 cggcacgggg cgccccggac aggagcgcgg cccgggccag ggggaaggat cccccggcga
     1321 ccagggcgaa ggatgccacg agggcggcca gtccgaaccg gggcgccgga cccgacccct
     1381 cgggggaact cattcctcct cgaatttcct gtcgaagagg agggccacgg cttccacggc
     1441 ctgggcttcg tcctccccct cggccaggac gcggagttcg accccgaacc ccgctcccag
     1501 ggtgatgatg cccatgatgg acttggcgtt gatgcggtcg gaattcttct cgatgaagat
     1561 cttggacttg aacttggagg cggtctggac gatgagggcc gcgggacgag cgtggatacc
     1621 cgcgcggttc ttgatggaaa cggacttctc ggtcatggat tcctcgctcg gcggcgtttc
     1681 ataggatgtc gtcctggccg aaatacacgg cccgcgcgtt ctcgctttcc agccaacgga
     1741 gaatgttgcg gttgaattcc ttggcggagt ggtagcccat cttctttagc cgctcgttca
     1801 tggccgcggt ctcgatgatg atggggatgt tccgccccgg cttcacgggt atgtccagct
     1861 gggggaccat gacccccagg atgtccacgg tcttttcgtc cgtgccgatc cggtcgtaga
     1921 ccttgtccgc gtcccactcc tccaggttgc agaccagctg gatctgcttc ttgtcccgga
     1981 tggcccccac gccgaagagg tgggtcacgt tgatgatccc caggccccgg atctccatgt
     2041 ggtgcccgac gatcttgttg gctccctggc ccatgaggta gttgccgttc acgcagcgga
     2101 tttccaccac gtcgtccgcc accaggcggt gcccccgctc gatcagctcc agggccgtct
     2161 cgctcttgcc caccccggaa tccccggaca acaggatccc gatcccgaag acctccacca
     2221 ggaccccgtg gatggaggtc ttgggggcga agatgtccga gagaacccgg aggatgcggc
     2281 cgacgaactc gctggaggaa agatcggtct gcaggatggg gcagttggcc gcctccgcgg
     2341 aggccaggaa ctcggcctgg ggggccaggc tgtgggtgaa gatgcagcag ggcatctcga
     2401 actcgaagac ccgcttggcg acgcccaggg agccctcccg ctcgagcttc tccaggtagg
     2461 cgtactcccc ccggccgaag acctggatgc gctggaaggc gaaggcgtcg aagaagcccg
     2521 agagggccag gccgggccgg ttgaggtccg gcacggagat ctgcctcccc aggcccttgc
     2581 gccccccgat gcagcgcagg ttcagggcat tgtgctcccg gaggtccagg ttgaggaggt
     2641 ccaatacgga gaaccggacg gtatccatgc tcgggggagt gtactacggg gagaggtgaa
     2701 tagtgaataa gtgaataagt gaataggcta attgggaaca gaggaagatt gcatggcgaa
     2761 gcggatgcat gcggcctctc gaagtccctc ccctattcac ctatccacga ttcacccatt
     2821 cacctcactt gttcctgtcc tgcaccttct ccttttcctt ccgtactttc atttccatct
     2881 tgtccataag cttgtcgatg cccgcggcca gttcgaaatc ccgttcctga atatgggcct
     2941 gatgccccca ccggaagtgg atggtggact cgcagatgaa atccttttcc ttggtgaacg
     3001 cgaagtcgac cgtgacgatg gtatccttcg cgaaattcag gcgctcgagc ttcttgtcca
     3061 ggtagtcccg ggtcggggcc cccagatcga agtggacgga ccgtacatcg acgttcatgc
     3121 gcacctccta gcgtatcgtg attccaggta ccggcggatc cgccggcgtc gtactgttcc
     3181 gcgtcgtgtt cggctcaagg cggaccctat tcgtgcctgc ggccgaagga ggagtccatg
     3241 ttcagttcca gccggtactt ggccacggtc cgccgcgcca ggttgatgcc cttgcgggcg
     3301 agctggtccg cgatgtcccg gtcggacaag gcccgggcct cgttctcgat gatctccttg
     3361 atgacctgct tgaccccctc cttggaaaag cgggatcccg aggacccggc cccggatatg
     3421 gagttggtga agaaatactt gagctcgaag atcccccagt cggtctggac gtacttccgg
     3481 ttggccagcc gggagatggt ggtctcgtgg accccgacct ccccggcgat gtcccggagg
     3541 gtcaggggcg ccaggtgctt gggtcccttg gtgaagaagg acctctggaa ctccacgatg
     3601 gcgcgggtgg ccttgagcag ggtcttgttc cgctggtgga tgctctggat gaaccacttg
     3661 gcccggcgga tgttctcctt cacgaaaacg tccgtggtcc ggtcgtgctt ctcctcggac
     3721 aggcggtcga agaagggatt gatgcccagg accggaatct cctcgtcgtt caggacgatg
     3781 acgaactccc catccttgag cttcacctgg atgtccggga tgacgtactg gggttccgag
     3841 gaggagtact gcctgcccgg gaagggattc agggtcttga tgaaggccag gatggaggac
     3901 aggtcggctt ccgagagctt gaggcgcttg cggatatcct cgtgcttgcc cttttccaga
     3961 agctcgatgt ggtcccgaag gacctccacg gtgcgggggg gggcctcggg gcacagttcg
     4021 gcctggacga tcagggattc ccggtagtcc gaggcgcaga ccccgacggg ctcgaagccc
     4081 cggaccaggg agagggcgga cttgaaggcg tcctccggcg tgtcccggca gaccaggcga
     4141 ggatcctcct tgtggaagcc gtcgctgtcc aggttctgga tgaggttttc cgcggccgca
     4201 cgcacctttt ccggcacggg ctggagccgg agctgccaca gcaggtgctc ctggagggtc
     4261 tccgcccggg agatggcccc ttccaggaac atgcgcttgg agtcgtcgtt gggatcccgg
     4321 ccccggccgt acccggggtc cgaggcgttc tcgtagaccc gggccgcgtc ggaatccgac
     4381 tcccgttcct cggggaagga ttccagggag accgtggagc ggtcctccag gacctccagg
     4441 gccggattgg cctcgatctc ctgctggatc tcgtccttga gttcgggaag gggcagggcc
     4501 atgagccgga tggattggat catctggggg ctgagtttct ggcgttgttc ctgtaccagc
     4561 accggcttct gaagctgcaa cgaaggctcc tcccgaattc ctatcttcaa atgttaccgc
     4621 cccaccccgg aaagtcaagg atcggggctc cgcggggccc ggtccggact tcccgggacc
     4681 gccggcgccc ccggcagacg gatggggttc gcatgcccgg gaacgctttt cccttgaccc
     4741 ttaaaaaaca tcctcggtat tttatggata cattattccc cataccaggt gatcgaatgt
     4801 ccaagacgtc caagaccgag agcaaaaccg aatccaaaac cacagccccg gagcaagcct
     4861 ccgggacggg cccccttccc gaggatgcct gcgaggccac cgaggacctt gcccgcatcc
     4921 tggaggcccg catggcggaa gcccagaagc gggccgcgga actccaggcg gagaactccg
     4981 aactcaagga ccagtacttg aggaaactcg cggattacga aaacttccgg aaacggatgt
     5041 tccgggaaaa ggacgacgcc gtccagttcg ccaactcggc cctcctgcac gaccttctag
     5101 gcatcctgga cgatttcgac cgggcggtct cgagctccga ggtgtcccgg gacttccagg
     5161 tactccatga cgggatcgtc atggtccgga accggatgct gtccaccttg gagggaaagt
     5221 acgggctggc ccgcttcgat tccgcgggag cctccttcga cccgaacgtc cacgaggcgg
     5281 tggccatgga gaagggcccc gtgccggacc ccgtggtcgc ggaggagttc cagaagggct
     5341 acaagctgca cggacgggtc ctccgggcgg cccgggtcaa ggtccggatg cccgacggct
     5401 ccgccccgga agccccggct ccggacgcgc cgcccccctc cgcgggggag acgaagtgaa
     5461 catgagtact atgcgccgaa gaggaataca aggagagtac ggaaatgggt agaatcatag
     5521 gcatcgacct cggcaccacc aactcctgcg tggccgtcat ggagggcgga gagcccctgg
     5581 tgatcgcgaa ctccgagggc cagcgcacca ccccgtccat cgtcgggttc accggcaagg
     5641 gcgagcgggt ggtcggccag cccgcgaaga atcagatgat cacgaatccg gagaacacgg
     5701 tctattccat caagcgcttc atgggacggc gcctcgagga agtccagacc gagatcaagc
     5761 tcgtgccgta cacggtcgtg tcgcaccaga acgacgtacg cgtcgcggtc tccgaaaagc
     5821 agtactcccc ccaggagatc agcgccttcg tcctgcaaaa gatgaagaag accgccgagg
     5881 actacctggg cgagaccgtc acggaggccg tcatcacggt gccggcgtac ttcaacgacg
     5941 cccagcgcca ggccaccaag gacgccggga agatcgcggg cctcgaggtc aagcgcatca
     6001 tcaacgagcc caccgccgcg gccctggcct acggcttcaa caaggaccag aagaaggaga
     6061 aggtgatcgc cgtgtacgac ctggggggcg gcaccttcga catctccatc ctggagctcg
     6121 gggagggggt cttcgaggtt aagtccacga acggcgacac ccacctgggc ggcgacgact
     6181 tcgaccgccg catcatgcag tggctgatcg acgagttcaa gaaggacacg ggcatcgacc
     6241 tgtcccagga caagatggcc ctccagcgcc tcaaggaggc gtcggagaag gtcaagatcg
     6301 aactgtccaa cgtccagcag gccgaggtca acctcccctt catcacagcc gacgcctcgg
     6361 gacccaagca cctccagaag tccctgaccc gggccaactt cgagcgcctg atcgaggacc
     6421 tcctggagaa gtccaaggat ccctgccgca aggcccttca ggacgcgggg atcaccgcgg
     6481 cccagatcga cgaggtcatc ctggtgggcg gctccacccg catccccaag gtccagacca
     6541 tcgtccggga actcttcggc aaggaaccct ccaagggggt caaccccgac gaggccgtgg
     6601 ccgtgggcgc cgccatccag ggcggcatcc tgggcgggga cgtcaaggac gtcctcctgc
     6661 tggacgtgac ccccctgtcc ctgggcatcg agaccctagg cggggtcttc acccggctca
     6721 tcacccggaa cacgaccatc ccctgccgta agagccagat cttctccacc gccagcgacg
     6781 ggcagacggc ggtctccatc cacgtcctcc agggcgagcg ggaaatggcc ggccagaacc
     6841 ggaccctggg ccgcttcgac ctggtgggca tacccgccgc gccccggggg gtcccccaga
     6901 tcgaggtgac cttcgacatc gacgccaacg gcatcgtcca cgtctccgcc aaggatctcg
     6961 ggaccggcaa ggagcagaag atccgcatcg aggcctccag cgggctcaac gacgacgaga
     7021 tcaacaagat ggtcaaggaa gcggagatgc acgcggagga ggacaagaag gagcgggagc
     7081 gcgcggaggt ccgcaacgag gcggacaccc tggcctacgc caccgagaag tccctcaaag
     7141 agtacggcga gaaggtcacc gcctccgacc gcgagtccat cgagaaggcc atcaaggagc
     7201 tccgggaggc cgtggggtcc aacgacaccg cccgcatccg ggaaaagatc gaggtcctga
     7261 agcaggcctc ctacaagctg gccgaggaga tctacaagaa cgtgggccag ggcgggcagg
     7321 ccggcgggga cgggcaggga ccccaggggt ccggacccca gggccagccc gagccggaat
     7381 cgggacccaa gaccgcggag gacgtggact accgcgtcgt cgacgaggac gagaagaaat
     7441 aggatccggc cggcgcggcc gccgttccgg cactgccggc ggcgccgccc cccggtccga
     7501 taaggacgtc acgtggcgaa acgggattac tacgaggtcc tgggggttcc caaggactcc
     7561 tccaaggacg acatcaagaa ggcctatcgg aagatggcca tcgccaacca tccggacaag
     7621 aaccccggaa acaaggccgc ggaggagcgg ttcaaggaag ccaccgaggc ctacgagatc
     7681 ctgggagacg accagaagcg ggccgcctac gaccagttcg gcttcgccgg agtggagggc
     7741 atgggcgggg gcgcgggggg ccagcacgac ttctcctcgg tcttccggga cttcgaggac
     7801 atcttcgggt tcggggactt ctccaacatc ttcgactcct tcttcggcgg cggccgggcc
     7861 gggggagccc ggggacggtc cggggtcggg aagggccagg atctccggta cgacctggag
     7921 ctgacattcg agcaggcggt cttcggcacc gccgtggaga tctcctacgc ccgcaacgat
     7981 tcctgcccca cctgcaaggg gaccggggct tcctccgggg gcggccgaaa ggtctgtccc
     8041 cagtgccagg gctccggtca ggtccgcagg agctcgggct ttttctccat agcctccccc
     8101 tgccccgcct gcggggggga cgggtacatc gtggagaacc cctgccggga ctgcgccggc
     8161 acggggctgg ccaagaaacg gcagaagatc aaggtgacca tccccgcggg ggtggacgac
     8221 ggaaaacgag tccagatacc cggacagggc gacacggggg ccaacggcgg cccccccggg
     8281 gacctgtacg tgttcatcca cgtccggccc cacgagttct tcgaacggga cggctacgat
     8341 ctctactgcg ccgtccccgt caccatcgcc caagccgccc tgggagccga gatcacggtg
     8401 cccaccatcg agggcaagaa gatcagcatc gccgtgcccg ccggggttcc ccacggcaag
     8461 gtcttgcgga tccgcgagga gggggttccc cacgctagct caaattcacg ccggggggac
     8521 atgtacgtga aggtgatgat ccaggtaccc gcaaagcttt caaagcgggg ccgggaactc
     8581 ctggaggaat tcgcccggat cgagaacgac cccacggatc cgcgccccct gcggctctcg
     8641 gagctgaaga acaactgagc atacggcccg ccccctcagg ggcgggtttt ttcttccagg
     8701 tccgccagcc aggccagggc gtattcccgg gactccccgc acatctcggg gtccgcgcgc
     8761 agggatccgc agaacgccgg tcgctccggc cttccccaga gggcgcagcg gaagtccacg
     8821 gtcaaatgag ggcaggggac gcccgcgggc ttcccctccg gcagggccgg caacgtcggg
     8881 gctatggagg gggcgacgca gcaggccccg cagccggacc tgcactcgaa agcggatttc
     8941 agttccggcg acgtatcggg catgccgtaa gcctaacgga gcgggctcga ggaggcaatc
     9001 cggaaccctc cgcccttgac caccgtcccc gggaagtgta tcaatacccg aaaggccggc
     9061 gtggcggaac ggcagacgcg gtggactcaa aatccactgt ccgcaaggat gtatgggttc
     9121 aagtcccatc gccggcatac cttacccttg tgcgcttcca gcgcatctcc ataatcgatg
     9181 ccggcgatgg gacttgatcc gagcgagcgg ccccggaagc gcgagttatc gagcgcttcc
     9241 ggggtgagcg cgccgagcgc gcgaacacga gcgaggcggc ccggagcggc gcctaacctt
     9301 ggcactcctt ccagcggaaa cagctcacca ggtggtcgtt cacgaggccc gcggactgca
     9361 ggaaggcata gatgatcacg gacccgacga agttgaagcc ccggcgtttc aggtcctcgg
     9421 agatcctatc ggacaggtcc gttcgggcgg ggacctcgga catggatttc caccggttca
     9481 cgacggaccg tccctccgta aagccccaaa gatagtcgga gaaggatccc cgttcctcgc
     9541 ggagggccag gaaggcgcgg gcgttccgga ccgcggagcc caccttgagg cggttccgta
     9601 cgatgcccgg atccgccagc agtcgggcct cgtccgcggg gccgtaccgg gcgatccgtt
     9661 cgggatcgaa cccctcgaag gcccggcggt acccttcccg gcgcttgagt atggtgatcc
     9721 aggaaaggcc ggcttgggag ccctccagga cgaggagctc gaagagggct cggtcgtccc
     9781 gcaggggcat gccccattcc tcgtcgtggt aacggacgta gtccggatcg gtcccggccc
     9841 agggacagcg tttcggtgcc atgcgcacag ggtagcgcgg gcggaggcag agggcaacag
     9901 gacacaggag ccaggcgatc ggttggcttc cagacgttcg gcgatcggga gaatccgcgg
     9961 tcgtcggctc ggccgtcgac cctgactcct gtccccggag tcctgagtcc cgttgactgt
    10021 ccacgattcc cgatctaccc tggtcgcaag aatcttctgg aggttgccat gccgaatctc
    10081 gtgcccggcc tgaccggaga gcgcaccctg atcgtggagg agcggcatac cgcggcccac
    10141 ctgggctccg gaggcgtacc cgtctacgcg acgcccatga tgatcctggc catggaggag
    10201 gcggccctgt ccgcggtgga tccgcggctg gagcccggtc aggtcacggt gggctaccac
    10261 ctggacgtga agcacctggc ggccaccccc ctggggatgc gcgtgaccgc ccgggcggaa
    10321 ctcctggccg tggacggaag gatgctgtcc ttcaaggtcg aggccttcga cgagaaggag
    10381 aagatcgggg agggcaccca cgtccgggcg atcatcaacg tcgccaagtt caaggaaaag
    10441 ctggcggcca agtccggcgt ctaggccgcc cgtgagcgct ccggcacggt ggtcgcgggg
    10501 gccgaggggt cggatttccg cgcggccgcg agcctatatc gctaaataaa ttgacatatc
    10561 aggccgcgcg tagtttactc ctaagaaagt gtcaccggga tcccccgcca ggaccccctg
    10621 ggccgaggcg ggaccacgaa tacccgaaac cgtaaaggag ccctctatga agaacaggtg
    10681 gaatcttctt tggatcctcc tcgccgccgc cctgatcgcg ggctgcgcga agaagtccga
    10741 gcccgccgcc gtcgcggcca aggccgagcc gtacaaggtc gccttcgtct acatcggccc
    10801 ccccggggac ctcggctgga cccatgagca cgaccagggc cgcaaggccg tggaggcgga
    10861 gttcggggac aaggtcaaga cggtctacat cgagaacgtg ccggagggcc ccgacgccac
    10921 ccgcatcatc cgccagtacg cccaggaagg ctacgacatg atcttcgcca cctccttcgg
    10981 ctatatggac ccgatggtcg aggtcgccaa ggagttcccg aaggtcaagt tcgagcactg
    11041 ctcgggctac aagaccgcgg agaacatgtc cacctacttc ggccggatct accagccccg
    11101 ctacctctcg ggcatcgtgg ccggcaagat gaccaagagc aacatcatcg gctacgccgc
    11161 cgccttcccg atccccgagg tcgtccgcgg gatcaacgcc ttcaccctgg gcgcccgctc
    11221 cgtcaatccc aaggtccagg tccgggtggt ctggacgaac acctggtacg accccgtcaa
    11281 ggagcgcgag gccgcggtgg ccctcctgga cgccggcgcc gacatcatcg cccagcacca
    11341 ggacacggtg gagccccaga aggccgccaa ggagcgcggg aagctctcca tcggctacga
    11401 ctcggacatg cgcgccttcg tgggcgacac ggtcctaacg agcccggtgt ggaactgggg
    11461 ttcctactac aaggatacca tcggcaaggc catggccggc acctggacca cccaccagta
    11521 ctgggagggc ctcaaggccg gcgtggtccg cctggcggac atgagccccc tcgttcccca
    11581 ggatgtgaag gacctggtca aggccgcgga gcagaagatc ctggacggga cctgggacgt
    11641 gttctacggc ccgatcaagg acaacaccgg cgccgtgaag gtggccgcgg gcgccaagat
    11701 gaccgacggc gagatgctct ccttcgactg gttcgtggaa ggcgtcgtcg gaaaggtaaa
    11761 gtaagacaag cacgggaagc ccggcggccc tcagggcccg ccgggtgtcc gtaccggagg
    11821 cctcccccgt gccggggagg tttttttcgc gggtccccgg cgacgctccg gcgtccgaag
    11881 cccggacaat tccgctcgac aaggtgattc ctatggacgc tagcgaaccg aaggtccccc
    11941 tggtggagat gaggggcatc cgaaaatcct tccccggggt cacggcctgc gacggcgtgg
    12001 acctccgcct gggccggggc gaagtcctgg ccctgctggg ggagaatggg gcgggcaaga
    12061 gcaccctcat gaacgtgctg gcgggcctgt accgccccga cgagggggag atccggatcg
    12121 gcggcaagcc ggtcgagatc cgttcccccc gggactcggc cgcccacggg gtgggcatgg
    12181 tccaccagaa cttcatgctg gtggacacca tgaccgtggc cgagaacgtc gtcctgggca
    12241 tggccgggct tcccctggtg cccgacatgg ccgaggtgaa gcgttccgtc ctggaactgg
    12301 cggaaaagta cggcctccgg gtggacccgg atgcctacgt ctggcagctt tccgtgggag
    12361 agcagcagcg cgtggagatc ctgaaacagc tctaccgcaa ggcggagatc ctggtcctgg
    12421 acgagcccac cgcggtcctc acaccgcagg agtccgagga gctcaacgtc gtggtccaac
    12481 gcatgaaggc ggagggcaag agcgcggtct tcatcaccca caagatggag gaggtgatga
    12541 ccttttccga ccgggtcatg gtgctccgga aaggaaaggt tgtagtggag aaggtcacct
    12601 ccgagaccag cccccgggac ctggccaagc tcatggtcgg aagggaggtc ctgttccggg
    12661 tggagaagaa gtccttcgag ccgggggcgg tcatcctgga gctcaaggat gtccgcgccc
    12721 tggacgaccg ggggcttccg gccctgcggg acctgtccct caaggtccgg gccggcgaga
    12781 tcctcggcat cgccggggtg gccgggaacg gccagcggga gctctccgag gtcatcacgg
    12841 gcctgcggcc ggtgagctcc ggggggatct ccatcaactg cgtggagacg acgaacaaga
    12901 cccccctcga gatcatccgg agcggggtca gtcacattcc ccaggaccgg atcgccgtcg
    12961 gcgccgtggg agacatgagc gtggccagca acctggccat gaagggctac cgggacgcgc
    13021 ccatttcccg gggggtcctg ctgttgccgc gtcgcatcct ggacctggcc cggcgcatga
    13081 tcgagagctt ccgcatcgcc accccctccc cggagacccg ggtgaagttc ctgtccgggg
    13141 gcaacatcca gaagaccatc ttggcccggg agatcgagtc ctgccgcagc ctcctggtgg
    13201 ccgtctaccc ctcccggggg ctcgacatcg gcgccaccga ggcggtccgc aaggagctgg
    13261 tggcccagcg ggacgcgggc atggcggtcc tcctggtatc cgaggacatc gaggagctcc
    13321 tatccgtggc cgacacggtt ctcgtcctct acgagggccg ggccatgggg accttcccct
    13381 ccgcggaggc ggatcccgag gagctcgggc tcctcatggc cggagtcgag cgcaaggaga
    13441 ccgtgtcatg agcatcctct tcgagaagcg caagaacatc tcccggaccg cccaggtcct
    13501 ggttcccgcg gcctccttcg tcgtgtccct gatcctaacg gggatcctgc tcctggcctt
    13561 cggggcggac cccttcgcca cgtaccgggc catgttccgg ggggccttcg ggagcggacg
    13621 ggccttcacg gagaccctgg tcaaggcgat tcccctgatg ctcaccggcc tgggcgtggc
    13681 cctggccttc cggctccggt tctggaacat cggagccgag ggccagctga ccctggccgg
    13741 ggtcgcggcg acgggggtgg ccatcttctg gggccggtgg atgccctcct tcctgctcct
    13801 gccgacggtc atcctggtcg gctgcctggc cggcgccgcc tgggcgggca tacccgccct
    13861 gctccggacc cggctgaagg tggacgagac cctggtcacc ctgatgctca actacgtggc
    13921 catcctcttc tcggagtacc tctactacgg cccctggcgg gaccccaaag gctacgggtt
    13981 cccgggcacg gagcccttcc ccgaggccgc ctggatgccc cgcatcgcgg gccgcgccca
    14041 cctgggcctc gtcttcgccc tgttcctggc cttggcgctc tggttcgtgc tgaaccgtac
    14101 ccgctggggc ttcgagctca agatcctcgg ggccagcccc caggctgcgc gctaccaggg
    14161 catctcggtg gagcgcaaca tc
//
LOCUS       DAOTEI010000087        14082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_2991_length_14082_cov_4.901832, whole genome shotgun sequence.
ACCESSION   DAOTEI010000087 DAOTEI010000000
VERSION     DAOTEI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 14082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14082
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_14082_cov_4.901832"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5313_09545"
     CDS             complement(<1..271)
                     /locus_tag="P5313_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRY80648.1"
                     /translation="MTLEAALARLDPSRPLPGEAARASMIPEYRKSPRSEDDGEPWRE
                     ASVLILLYPSDGRAVFPLIQRPPGAGIHAGQVSLPGGAREKGESAW"
     gene            complement(268..3498)
                     /locus_tag="P5313_09550"
     CDS             complement(268..3498)
                     /locus_tag="P5313_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY80649.1"
                     /translation="MRRAADLHIHSRHSIATSPKLTPVHLDRWARVKGLDLVGTGDCT
                     HPAWLEELGESLEPAGDGLYRLRPELRRAFDAGPALSENLPEPGTDRAVRFVLSGEIS
                     TIYSRDGRTRKVHHLVLLPDFEAARSFQARLARVGNVTSDGRPILGIDSRDLFHLLLD
                     SDPRAILVPAHIWTPWFSALGERSGFDSIEECYGDLADRITAIETGLSSNPPMNWAVG
                     SLDRFAIVSNSDAHSPEKLGREATILDMEDSWEGLTRALGGGGPAGRILETLEFFPQE
                     GKYHADGHRACGVVRMPGEDSWGPCPVCGKPLTPGVLGRVAALADRPVDESAPCPAEY
                     AGTNRRPYRSLIPLKEILAELIGAGEGSKAVASAYASLVREAGSEFGLLLDLDLREIG
                     AFRAPGIPGELLAEAVGRMRSGRVRIRPGYDGEYGEIRLFAPGERFEPRDGAGLFGPE
                     NAREGTIAAAGEAAAPIPARRGPRERPRNPAGKAGGSAEAPIPTDGSGPAVGIAPDPD
                     QELAVAHPGGPALVLAGPGTGKTSVLIRRIARVLKEGADPSSILALTFTNKAARELRD
                     RLERGPVTGPSGVVTASTFHAFCLGLLREHAADAGLPQGFRVFGEKERDALLAETAGK
                     RVRSLGAYVESRKRRLLLPGERTPRLGPGAPPDLLVLLKPEETAGGPGFDPDLDALYS
                     AYRARLKAAGALDFDDLVSGAVRLLAARPEILSSLRDRYLKVYVDEYQDVNLAQYALV
                     RVLAPPELYPEPGTEAAGPGLDPDAPAPRRELLVIGDPDQSIYSFRGADPRFIERFRQ
                     DYNGARVYSLSRSWRCPPAILGAAGRLAGRILSGSGTAAVLERREAPTERSEAEGIAR
                     RIDAMIGGTRFFALDSGTAGGADESSAPALRSLSECAVLVRTSAQIPALEQALRDHGL
                     PCRTADGYPGLEEDPSRAAPEGTDASAGAEPIAREAADPRDEAVRLLTVHAAKGLEFD
                     YVFVAGLEEGLFPFTLYDDPSERAASEARVGEERRLLYVALTRARKGLSLWRAKARTF
                     RGRKVALPPSRFLAEIEDLVPAVGGDIDYRRDPQGSLFPADREDRT"
     gene            3894..5426
                     /locus_tag="P5313_09555"
     CDS             3894..5426
                     /locus_tag="P5313_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY80650.1"
                     /translation="MVYVERRRPGWNVRGARALAAVSLLVFLASGGYALGSKDGEIRD
                     PSSRDLGAFSQGPYGTAPEPSGTWSLAVPTTPKPEVQPAEPSVTGTPRKMTVEEAVAV
                     ARDGNLGLVSPRIAAEGKRRADALAWNNFLPTVDVSGTLGRWNVDQSSMILGPVPRWS
                     LSASFSAQLMLNLALFEGVKSLRADYEAGLISYAQAEARLERDVRKSFYNLLLLEENM
                     KLMEEQIEAARRRYEQARANYRAGTAPELSMLQAQVAYENLKPALQEMRIGYQAARDA
                     FAMTLGLPRGTEVQPAGKIEPAYAKLDADAMIASGLSSRLDIQSLVKSLESLQIAETG
                     LKYRLWTPNLILGWNLDPAFSGDPWADDLFDGDRWSQRSGMFRATLSMRLNGLLPFTQ
                     DGQQLAELRDQRESLRANLAQALRGAEMEIDGLVRRLEKSRTSHTALELNAGLAERAY
                     RLSEEAYRAGAADLLDVQNAELELRKARLELLKEDYTYVTALLDLEYAVGARFGSLGG
                     SK"
     gene            5427..6407
                     /locus_tag="P5313_09560"
     CDS             5427..6407
                     /locus_tag="P5313_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY80651.1"
                     /translation="MNANTEKKTIARGLKTALAVLACAGLAAGTMLVLSSCSAAKKKA
                     EAQAPAAPETVFAVETAPVVRGPVRDYIALSGDIVASSTVDAYSDVAGKVTRHFVSVG
                     QRVEKGEALIEVDPSRPGMSYQPGIVKAPISGTVTSLPMEVGSTIAPSVPAARIARTE
                     SLELRTYAPERFVSKMRAGLAADVSLAAWPGEVFPAVLREVAPVLDPTSRTLELRLAF
                     RRPDPRLKAGMFASVKVVTEDKASVVKVPAEAVVKRFGETFAFVAEEDPAKPGSHVAR
                     KRAVTPGILIDGIYEIREGLAEGESVVVRGQTLLDEGVAVNVVLRRPAAR"
     gene            6418..9678
                     /locus_tag="P5313_09565"
     CDS             6418..9678
                     /locus_tag="P5313_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY80652.1"
                     /translation="MSLTRTVVGKPTTIFVGFALLVALGFFATVNLAVDLYPEIEPPV
                     ILIFTGYRGAGPEEIEKTVTRPLEGALSNTQGIEKITSTSSAGSSMVILEFTYGTDMA
                     EAANSVRDNLEFVKGALPDEADTPMIFKFDPAMIPIMGLMVSGNRSPEELREVAEKTI
                     QPRIEQVQGVALAQVSGGRQRVVRVEVPLERLEAYGLTINQIVNMLRGQNVQVAAGSI
                     VDGNLDYLLTAPGEYKSIEEIGDAVIAYKGGGYDPVKGTQPARKVRLRDIASVFDGYR
                     DESNLVFVNGVPAVQITIQKQSGKNSVQTANAVRARLVRLAGELPPGVKVTELFNTTD
                     IIENSIANVATSARDGAILAVVILFIFLRSWKPTLVIGVTIPISLLFTLMLMYFAGLT
                     LNIMTLAGLALGVGMLVDNSIVILENIYRYREKGAKLRISAILGTQEMVTAIMASTLT
                     TICVFAPLIMFGKKLDIVGELFSGLAFTVVISLATSIAVAVLLIPVLSSHYFPLVTRA
                     QKPLRSPFLRKIDEVMGRFFAALDEGYRRGVKLVMRHKLATIGLIAAVFAGTMAYAPK
                     IGFVFMPPMDQDAVVVNVDMPLGTRLAETEAVLRQFEGIVEREVRGFEKIVMQVGQGS
                     MFGFLGANSKNRGSLRIMLPPFEQRIDDSDAIKEKLRAHFNDVPAAVFSFGTDQGGFG
                     GGSPIDILVKTEDLALGKRTAEAIRDLIKREIPEITEPSIDLADGLPQVEIRIDRQRV
                     YDLGLNIYSIATEIKAAVDGVAATKYRSGGSEYDILVILPEADRSELPALDRMFVVNP
                     AGTRVPLASFASYEKTTGPIDIKRENQGRVVHVTAGVKPGVPLDVVEGKMRSLIAREI
                     PADDAVIIEYGGDWKQFLEYGGTFALIVLAATLLVFGVMASQFESFLDPFIIIFTIPL
                     SFTGVVWIYALTGDIFNILTAVGLLVLVGVIVNNGIVLVDYTNLLRKRGLHLEEAVIT
                     AAGNRLRPILMTTLTTVLALLPLAFYPGEGTELVAPIGKTVLGGLTFGTLMTLFLMPA
                     IYSVFNRKSEERARKRDERLNRIAAGQKGRVKATVPAGAAETIPADDAAGTEGGRA"
     gene            9675..9950
                     /locus_tag="P5313_09570"
     CDS             9675..9950
                     /locus_tag="P5313_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80653.1"
                     /translation="MKRVEIVANRSVQEDLMDALAEAGVARYHTLIPAAHGTGRQGMR
                     TGEAAWPEENFVLFTYCEEAEARTIADVVARIKKAFPKEGIKYFELG"
     gene            complement(10081..10695)
                     /locus_tag="P5313_09575"
     CDS             complement(10081..10695)
                     /locus_tag="P5313_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80654.1"
                     /translation="MALIKSALEIALERTKDMKADPAALKAFELKQEGQKLAGEFLSD
                     PGSVDLEARIKALPKEDRGILKSAIFGVLAARIQLPLTKTGIPPETLDALAKGFTALA
                     ASPFPDKRIGGLMKQVGDFLARYLEDATNLDTALRKQFEPKLRRKEQELAARTGQSVR
                     LDPLSDPEFLKVYQQNVGHLKAQYQSALDQAKEDLAAMLGIEKD"
     gene            complement(10760..11596)
                     /locus_tag="P5313_09580"
     CDS             complement(10760..11596)
                     /locus_tag="P5313_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY80655.1"
                     /translation="MDDRARLSRSIYLSVTAFLVFQILLFLVFFRAYRMSGSYLRLFL
                     SVTAIFHLAILAFLLAFRKQFALVEGGRSLVRVNLANEVTLARMSTLPTLLILIVASK
                     EYSIRAPLLVLVALVFVTDFADGWISRASRQVTFMGRMLDAISDYSVLIVLSIVFYYY
                     ELIPGWFFWVVIFRLVFQGAGMAVLLMARRPIRTQSTLMGKAAIFSTMALYTLEVLRV
                     MVRWEAPGAWRVLEYAAGSVVFLSIFEKAVLLFVQLRRIPKAGAQPPAGSSSSEAKAG
                     EA"
     gene            complement(11645..12223)
                     /locus_tag="P5313_09585"
     CDS             complement(11645..12223)
                     /locus_tag="P5313_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY80656.1"
                     /translation="MTLPSLVTTVRVGLSPLFFALFITLTKNGEAPLWATLLYWGFFI
                     VMEITDLLDGIIARKMGSVTDFGKFYDPFADSFSRLTYFLAFLVTGFLPAWVFLLVLY
                     RDITVSFLRLLAMRRSIVISARPSGKIKAVIYAVACGSVLTLVTARAVPALAFSVPAL
                     GWIQKGLMWITAATAVWTTLDYSRGLKPDARA"
     gene            12259..13071
                     /locus_tag="P5313_09590"
     CDS             12259..13071
                     /locus_tag="P5313_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRY80657.1"
                     /translation="MNGEGRDPGRIEGMEAAKAAEVRFVLFDIDDTITEDGRLSEESY
                     SALWRLARAGIAAVPVTGRPAGWCDLIARQWPVDAVVGENGAFAFYMEDGRLEHLFHP
                     EARDPGSARARLEPLRDLVLSDVAGARVAKDQFSRLFDLAIDFAEEEPVLPLEAAVRI
                     RDLCESLGARAKISSIHVNAWFGAYDKLSMAELYLARRHGWDPVRDLGRVLYFGDSPN
                     DEPMFARFPLSVGVANVRRFLPLMKSRPTFITAGSHGRGFAEGVDLLLGLRG"
     gene            13179..13949
                     /locus_tag="P5313_09595"
     CDS             13179..13949
                     /locus_tag="P5313_09595"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY80658.1"
                     /translation="MKSAFRITFVYLAVASAWILLSDRAIALFTDDVAALTAYSSAKG
                     LFYVTVTSLMLFFMIRKEIAVRNDAITRLNRTLEVKGELIHELHHRIGNNIQVILGVL
                     GLETREGDFSAEAKDRIVHRLLAMRSVYDIVYDYEDMREISLRKVLAAYARTASRNVR
                     VRDDFPETPLPIETMVTLLLVLDIVLEALRESGYGGTAEMSSPEERTLTLRMDGYSGD
                     AGNFLGRNETFVRAYLKSIRGEIDLLPGDPAEIRVRYM"
BASE COUNT         2361 a         4797 c         4705 g         2219 t
ORIGIN      
        1 cccaggcgga ctcccccttt tcccgggcgc cgccgggcag ggacacctgg cccgcgtgaa
       61 tccccgcgcc ggggggtctc tggatgaggg ggaagacggc ccggccgtcc gagggataca
      121 ggaggatcag gaccgaggcc tccctccagg gttcgccgtc gtcctccgac cgggggcttt
      181 ttcggtactc ggggatcatg gaagcccggg ccgcctcccc ggggaggggc cgggagggat
      241 ccaggcgggc cagggcggct tccagggtca tgtccggtcc tctcggtccg cggggaacag
      301 ggacccctgg gggtcccgcc gataatcgat gtccccgccc acggccggca cgaggtcctc
      361 gatctccgct aaaaagcggc tgggaggcag ggcgaccttc cgtccccgga aggtccgagc
      421 cttggccctc cacagggaaa gccccttccg ggcccgggtg agggccacgt agaggagcct
      481 ccgctcctcc ccgacccggg cctccgacgc cgccctctcc gacggatcgt cgtacagggt
      541 gaaggggaag agcccctcct ccaggcccgc cacgaagacg taatcgaact cgaggccctt
      601 ggccgcgtgg acggtcagga gccggacggc ctcgtcccgg ggatccgcgg cctctcgggc
      661 gatcggctcc gctccggccg acgcgtccgt tccttccggg gcggctcggg agggatcctc
      721 ctccagcccg gggtacccgt ccgcggtccg gcagggcagg ccgtggtccc ggagggcctg
      781 ctccagggcc gggatctggg cggaggtccg cacgaggacc gcgcattccg agaggctccg
      841 cagggcgggc gccgatgact cgtccgcgcc tcccgcggtt ccgctgtcca gggcgaagaa
      901 ccgggtcccc ccgatcatcg cgtcgatccg gcgggcgatg ccttccgcct cggacctttc
      961 cgtgggagcc tcccggcgct ccaggaccgc ggccgtcccc gaacccgaga ggatccgccc
     1021 ggccagccgt cccgcggccc cgaggatggc cggggggcag cgccagctcc gggacaggga
     1081 atagacccgg gcgccgttat aatcctgccg gaaccgctcg atgaaccggg gatccgcccc
     1141 ccggaaggag tagatggact ggtcggggtc cccgatgacc agaagttccc ggcggggggc
     1201 cggcgcatcc ggatcgaggc cggggcccgc agcttccgtg cccggctccg ggtagagctc
     1261 cgggggcgcc aggacgcgga ccagggcgta ctgggccagg ttcacgtcct ggtactcgtc
     1321 cacgtagacc ttgaggtatc ggtcccggag ggaactcagg atttccggcc gggcggccag
     1381 gagccggacg gccccggaga ccaggtcgtc gaagtccagg gcccccgccg ccttgagccg
     1441 ggcccggtac gccgagtaca gggcgtccag gtccgggtcg aagccggggc cgcccgccgt
     1501 ctcctccggt ttgagcagga cgagcaggtc cgggggagcc ccgggtccca ggcggggcgt
     1561 ccgctccccg gggaggagga ggcggcgctt cctcgattcg acgtaggcgc ccagggaacg
     1621 gacccgcttc ccggcggtct cggccagcaa ggcgtcccgc tccttctctc cgaacacccg
     1681 gaagccctgc ggtagtcccg cgtccgcggc atgttcccgc aggaggccca ggcagaaggc
     1741 gtggaaggtg gaagccgtca cgacccccga cggtccggtc accggacccc gttccagccg
     1801 gtcacggagc tcccgggccg ccttgttcgt gaaggtcagg gccaggatcg aggagggatc
     1861 ggcaccctcc tttaacacgc gggctatccg ccggatcagg accgaggtct tgcccgtgcc
     1921 cggtccggcc aggaccaggg ccggaccgcc ggggtgggcc accgcgagct cctggtccgg
     1981 atccggcgca atgccgacgg cgggcccgga accgtccgtc gggatcggcg cctcggcgct
     2041 cccgcccgcc ttcccggccg gattccgggg ccgctcccgg ggcccgcgcc gggcgggaat
     2101 cggagccgca gcctctcccg ccgcggctat cgtcccctcc cgggcattct ccggcccgaa
     2161 gagccccgct ccgtcccggg gctcgaaccg ctcccccggg gcgaagaggc ggatctcccc
     2221 gtattccccg tcgtacccgg gccggatccg gacccgcccc gagcgcatcc gccccaccgc
     2281 ctcggccagg agttccccgg ggatccccgg ggcccggaag gccccgatct cgcgcaggtc
     2341 caggtccagg agcagtccga actcgctgcc ggcctcccgt accaatgacg cgtaggcgga
     2401 ggccacggcc ttcgaaccct cccccgctcc gatcaattcc gccaggatct ccttgagggg
     2461 gatcagggag cgatagggcc tgcggttcgt gcccgcgtac tccgccgggc agggggcgga
     2521 ctcgtccacg ggccggtccg ccaaggcggc caccctcccc aggacccccg gggtcagggg
     2581 cttgccgcag accgggcagg ggccccagga atcctccccg ggcatccgga ccaccccgca
     2641 agccctatgg ccgtcggcgt ggtacttgcc ctcctggggg aagaactcca gggtctccag
     2701 gatccgcccc gccggccccc cgccccccag ggcccgggtc aggccctccc aggaatcctc
     2761 catgtccaga atggtcgcct cccggcccag cttttccggg gagtgggcgt cggagttgga
     2821 gacgatcgcg aagcggtcca gggaacccac ggcccagttc atgggagggt tggaggacag
     2881 tcccgtctcg atcgccgtga tccggtccgc caggtccccg tagcactcct cgatggagtc
     2941 gaagccggac cgttccccca gggcggagaa ccagggggtc cagatgtggg cgggcaccag
     3001 gatcgcccgg gggtcggagt ccaaaaggag gtggaagagg tcccgggaat cgatccccag
     3061 gatgggccgc ccgtcggagg tgacgtttcc gacccgcgcc aggcgcgcct ggaaggaacg
     3121 cgccgcctcg aaatccggaa ggaggaccag gtgatggacc ttccgggtgc ggccgtcccg
     3181 ggagtagatg gtgctgatct cgccggagag gacgaagcgg acggcccggt ccgtaccggg
     3241 ctccggcagg ttttccgaca gggccggccc cgcgtcgaag gcccggcgca attccggccg
     3301 cagccggtac aacccgtccc ccgcgggttc cagggactcc ccgagctcct ccagccaggc
     3361 gggatgggtg cagtccccgg tgccgaccag gtccaggcct ttcacccggg cccagcggtc
     3421 caggtggacg ggggtcagct tggggctggt ggctatggaa tgccgggaat ggatgtgcag
     3481 gtccgccgct cgtcgcatgg aggattctag tccggactca ggggacaggc aacaggagtc
     3541 aagcgggaga aagtccgccg gccccggcgg actgatgtgt gatttcgggg ctgtccaata
     3601 agtaaccgac ttattggaca ccccggcccc gggaagttcg ggaactgtgt tcccgaactt
     3661 cccgcgaaat accgcaccat ctacgacttt tttcttaaat acgcgtaaga aacgctcggg
     3721 actcttgtca ttcccggccc ggagcgatac attttcccca tccggggctc acccggatca
     3781 cgggctatgg cccgtggccg tgtcgcgcga cagcggaccg gcgaacgctg gacggattcg
     3841 cgcagccggt ggtaccgggc cgggggcgga ttcggtccga atgggagcac agaatggtat
     3901 acgtcgaacg tcgacgcccc ggatggaatg tccggggggc gcgtgccctc gcggccgtgt
     3961 ccctcctggt attcctcgca tccggcggct acgccctggg atcgaaggac ggagagatcc
     4021 gggatccttc ctcccgggac ctgggggcct tctcgcaggg accctacggc acggctccgg
     4081 agccctccgg aacctggtcc ctcgccgtac cgacaacccc gaaaccggaa gtacagcccg
     4141 ccgaaccctc cgtcaccggg acgccccgga agatgaccgt ggaggaagcc gtggcggtcg
     4201 cccgggacgg caacctgggc ctggtgtccc cgcgcatcgc cgcggagggc aagcgccggg
     4261 cggacgccct ggcctggaac aacttcctgc ccacggtgga cgtttcgggg accctgggcc
     4321 ggtggaatgt tgaccagtcc tccatgattc tcggtcccgt gccccgctgg agcctctcgg
     4381 cctccttctc cgcccagctc atgctgaacc tggccctctt cgagggggtg aagtccctgc
     4441 gggcggacta cgaggcgggg ctgatatcct acgcccaggc ggaagcccgg ctggaacggg
     4501 acgtccggaa gagcttctac aatctcctcc tcctggagga gaacatgaag ctcatggagg
     4561 agcagatcga ggccgcccgc cggcgctacg agcaggcccg ggccaactac cgagccggca
     4621 cggctccgga gctctcgatg ctccaggccc aggtggccta cgagaacctc aagcccgccc
     4681 tccaggagat gcggatcggc taccaggccg cccgggacgc cttcgccatg accctgggcc
     4741 tgccccgggg gaccgaggtc cagccggccg ggaagataga acccgcgtac gccaagctgg
     4801 acgcggacgc gatgatcgcc tcgggcctct cctcccggct ggacatccag tccctggtca
     4861 agtccctgga gtcgcttcaa atcgcggaga cgggcctcaa gtaccggctc tggaccccca
     4921 acctcatact agggtggaac ctggacccgg ccttctccgg ggatccctgg gcggacgacc
     4981 tcttcgacgg ggaccggtgg tcccagcgct cgggcatgtt ccgcgccacg ttgtcgatgc
     5041 gcctgaacgg ccttctcccc ttcacccagg acggtcagca gctcgccgag ctgagggacc
     5101 agcgggagag cctccgggcg aacctggccc aggccctgcg gggcgccgag atggagatcg
     5161 acggcctggt gcgccggctg gagaagtccc ggacaagcca caccgccctg gagctcaacg
     5221 cgggcctggc ggagcgggcc taccgcctct ccgaagaggc ctaccgggcc ggggcggcgg
     5281 atctgctgga tgtccagaac gcggagctcg agctgcgcaa ggcgcgcctc gagctgctca
     5341 aggaagacta tacctacgtc accgctctcc tggatctgga gtacgcggtg ggagcccggt
     5401 tcggaagcct gggcgggagc aaataaatga acgccaatac cgagaagaaa accatcgccc
     5461 ggggcctcaa gaccgccctg gccgtccttg cctgcgccgg actggccgcg ggcacgatgc
     5521 tcgtcctttc ctcctgctcg gccgcgaaga agaaggccga ggcccaggca cccgcggcgc
     5581 ccgagacggt cttcgccgtg gagaccgccc ccgtggttag gggcccggtg cgggactaca
     5641 tcgccctctc cggcgacatc gtggcctcct ccacggtgga tgcctactcc gacgtggccg
     5701 gcaaggtcac ccggcacttc gtgtccgtgg gccagcgggt ggagaagggc gaggccctga
     5761 tcgaggtgga cccctcccgc cccgggatga gctaccagcc cgggatcgtg aaggccccca
     5821 tttcggggac cgtcacctcc cttcccatgg aagtgggctc caccatcgcc ccctccgtcc
     5881 ccgcggcccg gatcgcccgg accgagagtc tggaactgcg gacctacgcc cccgagcgct
     5941 tcgtctccaa gatgcgagcg ggcctggccg cggacgtctc cctggccgcc tggcccgggg
     6001 aggtcttccc cgcggtcctt cgtgaggtgg ccccggtcct ggacccgacg tcccggaccc
     6061 tggagctccg tctggccttc cgacgccccg acccccggct caaggccggt atgttcgcct
     6121 ccgtcaaggt cgtcaccgag gacaaggctt cggtggtcaa ggtccccgcg gaggccgtgg
     6181 tcaagcgctt cggcgagacc ttcgccttcg tcgccgagga ggacccggcc aagcccggct
     6241 cccacgtggc ccgcaagcgg gccgtgaccc ccggcatcct gatcgacggg atctacgaga
     6301 tccgggaagg gctggccgag ggcgagagcg tggtggtgcg gggccagacc ctgctggacg
     6361 agggggtcgc cgtgaacgtg gtcctccgcc gccccgcggc ccggtaaggg ggaccccatg
     6421 agcctgaccc gaaccgtggt cggaaaaccg accaccatat tcgtaggttt cgccctcctc
     6481 gtggccctgg gcttcttcgc caccgtcaac ctggcggtgg acctctaccc cgagatcgag
     6541 cccccggtca tcctgatctt cacggggtac cggggcgcgg gcccggaaga gatcgagaag
     6601 accgtgacgc ggcccctgga gggcgccctc tccaacaccc agggaatcga gaagatcacc
     6661 tccacctcct ccgcgggctc cagcatggtc atcctggagt tcacgtacgg cacggacatg
     6721 gccgaggcgg ccaattccgt ccgggacaac ctcgagttcg tgaagggcgc cctgccggac
     6781 gaggcggaca cccccatgat cttcaaattc gatcccgcca tgatccccat catgggcctc
     6841 atggtgtccg ggaaccgcag ccccgaggag ctccgggagg tggccgaaaa gacgatccag
     6901 ccccggatcg agcaggtcca gggcgtggcc ctggcccagg tgtccggcgg ccgccagcgg
     6961 gtggtccgcg tggaggttcc cctggagcgc ctggaggcct acggcctcac gatcaaccag
     7021 atcgtgaaca tgctccgcgg gcagaacgtg caggtggcgg ccggctccat cgtggacggc
     7081 aacctggatt accttctgac cgcccccggg gagtacaaga gcatcgagga aatcggcgac
     7141 gccgtgatcg cctacaaggg cggcggctac gacccggtca aggggaccca gcccgcccgc
     7201 aaggtccggc tacgggacat cgccagcgtc ttcgacgggt accgggacga gtccaacctg
     7261 gtcttcgtga acggcgtccc cgccgtgcag atcacgatcc agaagcagag cggcaagaac
     7321 tccgtgcaga ccgccaacgc ggtccgagcc cggctggtac ggctggcggg ggagctgccc
     7381 cccggcgtca aggtgaccga gctcttcaac accacggaca tcatcgagaa ctccatcgcc
     7441 aacgtcgcga cctcggcccg ggacggggct atcctggcgg tcgtcatcct gttcatcttc
     7501 ctgcggagct ggaagccgac cctcgtgatc ggcgtcacca tacccatctc gctgctcttc
     7561 accctaatgc tcatgtactt cgcgggcctg accctcaaca tcatgaccct ggcgggcctg
     7621 gccctgggcg tcgggatgct ggtggacaac tccatcgtca tcctggagaa catttaccgg
     7681 taccgggaga agggcgccaa gctccggatc tccgccatcc tcgggaccca ggagatggtc
     7741 accgccatca tggcctccac cctgaccacg atctgcgtct tcgcccccct catcatgttc
     7801 gggaagaagc tggacatcgt gggagagctc ttctcgggct tggccttcac ggtggtgatc
     7861 tccctggcca cctccatcgc cgtggcggtc ctcctgatcc ccgtcctctc gagccactac
     7921 ttccccctgg tcacccgtgc ccagaagccc ctgcggtcgc ccttcctgcg gaagatcgac
     7981 gaggtcatgg gccgcttctt cgcggccctc gacgagggct accgccgggg ggtgaagctc
     8041 gtcatgcggc acaagctggc gaccatcggg ctcatcgcgg cggtcttcgc cgggaccatg
     8101 gcctacgccc ccaagatcgg cttcgtgttc atgcccccca tggaccagga cgccgtggtg
     8161 gtgaacgtgg acatgcccct gggcacccgc ctggccgaga cggaggccgt cctgcgtcag
     8221 ttcgagggca tcgtggagcg ggaggtccgg ggcttcgaga agatcgtcat gcaggtcgga
     8281 caggggtcca tgttcggatt cctgggggcc aattcgaaga accggggttc cctgcggatc
     8341 atgctgcctc ccttcgagca gaggatcgac gactccgacg cgatcaagga aaagctccgc
     8401 gcccacttca acgacgtgcc cgcggcggtg ttctccttcg gcaccgacca gggcgggttc
     8461 ggcgggggca gccccatcga catcctggtc aagaccgagg acctggccct gggcaagcgc
     8521 accgcggagg ccatccggga cctgatcaag cgcgagatcc ccgagatcac ggagccctcc
     8581 atcgacctcg cggacgggct gccccaggtg gagatccgca tcgaccgcca gcgggtctac
     8641 gacctgggcc tcaacatcta ctccatcgcc accgagatca aggccgcggt ggacggggtc
     8701 gcggccacca agtaccgctc gggcggctcg gaatacgaca tcctggtgat cctgcccgag
     8761 gcggaccgca gcgagcttcc cgccctggac cggatgttcg tcgtgaatcc cgccggaacc
     8821 cgggtgcccc tggccagctt cgcgagctac gagaagacca cgggccccat cgacatcaag
     8881 cgcgaaaacc agggccgggt ggtccacgtg accgcggggg tcaaacccgg ggtacccctg
     8941 gacgtggtgg agggcaagat gcggtccctc atcgcccggg agatccccgc ggacgacgcg
     9001 gtgatcatcg agtacggagg ggattggaag cagttcctcg agtacggcgg gaccttcgcc
     9061 ctcatcgtgc tggccgccac cctcctggtg ttcggggtga tggccagcca gttcgagtcc
     9121 ttcctggatc ccttcataat catcttcacc atccccctgt ccttcacggg ggtggtctgg
     9181 atatacgccc tgaccgggga catcttcaac atcctgaccg ccgtgggcct cctggtcctg
     9241 gtgggcgtca tcgtgaacaa cggcatcgtc ctggtggact acaccaacct cctgcgcaag
     9301 cgggggttgc acctcgagga agccgtgatc accgccgcgg gcaatcgcct ccggcccatc
     9361 ctgatgacca ccctgaccac ggtgctggcc ctcctgcccc tggccttcta tcccggcgaa
     9421 gggacggagc tcgtggcccc catcggcaag accgtcctgg gaggcctgac cttcggtacc
     9481 ctcatgaccc tcttcctgat gccggccatc tactccgtgt tcaaccggaa gagcgaggag
     9541 cgggcccgga agcgggacga gcggctcaat cggatcgccg cgggccagaa gggccgcgtc
     9601 aaggcgacgg tcccggccgg ggccgcggag acgattccgg cggatgacgc cgccggaacg
     9661 gaaggaggcc gggcatgaag cgggtcgaga tcgtagccaa ccgttccgtc caggaggacc
     9721 tgatggacgc cctggcggag gccggggtcg cccggtacca taccctgata cccgcggccc
     9781 acggcaccgg ccgccagggc atgcgcaccg gggaagccgc ctggccggag gagaatttcg
     9841 tcctgttcac ctactgcgag gaggcggaag cccggaccat cgccgacgtg gtggcccgga
     9901 tcaagaaggc cttccccaag gaagggatca agtacttcga attgggctag gtttaaaaat
     9961 tctaccacag agtcacagag acacagagga agaagaatat gggggaaata ctccacccaa
    10021 gttctttaac tttgtgcctc tgtgactctg tggtttcttg ttactaaaac ctaaatcgtc
    10081 tcaatctttc tctattccca gcatcgccgc caggtcttcc ttggcctggt ccagggcgga
    10141 ctggtactgg gccttcagat gccccacgtt ctgctggtag accttcagga actccgggtc
    10201 cgacaaggga tccagccgta ccgactggcc cgtgcgggcg gccagttcct gttccttcct
    10261 ccgcagcttg ggttcgaact gcttccgcag tgcggtatcc aggttcgtgg cgtcctccag
    10321 gtaccgggcc aggaagtccc cgacctgctt catgaggccg cctatgcgct tgtccgggaa
    10381 gggggacgcg gccagggcgg tgaaaccctt ggccagggcg tccagggtct ccggggggat
    10441 ccccgtcttg gtcagaggca gctggatccg ggcggccagc accccgaaaa tcgcggactt
    10501 gaggatgccg cgatcctcct tgggcagggc cttgatccgg gcttccagat ccacggagcc
    10561 gggatcggaa aggaattccc ccgccagctt ctgaccctcc tgcttcagct cgaaggcttt
    10621 cagggccgcc ggatccgcct tcatgtcctt ggtccgctcc agggcgatct ccagcgcgct
    10681 ttttatcaat gccatgggtt tgctccttta ttcggccgcc ggctcgacgg tcggcggctt
    10741 ctgggaatga tcggtcgttt tatgcctctc cggccttggc ttccgatgag gaggatcccg
    10801 cgggaggctg ggcgcccgcc ttgggtatcc gccgcaattg aacgaacagg agcacggcct
    10861 tttcgaagat ggacaggaac acgacacttc ccgccgcgta ttccaatacc ctccaggctc
    10921 cgggggcttc ccaccggacc atgactcgca gaacctccag ggtgtagagg gccatggtgg
    10981 agaagatcgc ggccttcccc atgagggtgg attgggtccg tatcggccgc cgggccatca
    11041 gcagtacggc catccccgcc ccctggaaga ccagccggaa gatcacgacc cagaagaacc
    11101 atccgggtat gagttcataa tagtaaaaca cgatggacag gacaatgagg acgctgtaat
    11161 cgctgatcgc gtccagcatg cggcccatga aggtgacctg ccgggaagcc cgggagatcc
    11221 atccgtccgc gaagtcggtc acgaagacca gggccaccag caccagcagg ggagcccgga
    11281 tggagtactc cttggacgcc acgatcagga tcaggagggt cggaagggtg gacatgcggg
    11341 ccagggtgac ctcgttggcc aggttgaccc ggaccaggct gcgaccgccc tccaccaggg
    11401 cgaattgctt gcggaacgcg agcaggaagg ccaggatggc caggtggaag atggcggtga
    11461 cggacaagaa caaccggagg tacgagccgg acatgcggta cgcgcggaag aagaccagga
    11521 agagcaggat ctggaagacg aggaaggccg tcacgctgag atagatgctg cgggagagcc
    11581 gggctcgatc gtccatatcc tgatgcaccc tcatgaccgt tccgatgccg cggccgcctg
    11641 cggactaggc ccgggcgtcc ggtttcagcc cccgcgagta atccagcgtc gtccagaccg
    11701 ccgtggcggc ggtgatccac atcagcccct tctggatcca gcccaaggcg gggacggaaa
    11761 acgccagggc cggcacggcc cgcgcggtga ccagggtcag cacggatccg caggccacgg
    11821 cgtagatcac cgccttgatc ttgccgctgg gccgggcgga tatcacgatg ctcctccgca
    11881 tggccaggag gcgcaggaaa gagaccgtga tgtcccggta caggaccagc aggaagaccc
    11941 aggcgggcag gaagccggtc accaggaagg ccaggaaata ggtcagtcgg ctgaaggaat
    12001 ccgcgaaggg atcgtagaat ttcccgaagt ccgtgacgga tcccatcttt cgggcgatga
    12061 ttccgtccag cagatcggtg atctccatca ctatgaagaa gccccagtag aggagggttg
    12121 cccagagggg ggcttccccg ttcttcgtga gcgtgataaa aagcgcgaag aacagggggg
    12181 aaagtcccac ccggacggtc gtgacgaggc tgggaagggt catggggcga tgatacccgg
    12241 gaggtattag aggtgtcaat gaacggcgag gggcgggacc ccgggcgcat cgaggggatg
    12301 gaggccgcca aggccgcgga ggtccggttc gtcctcttcg atatcgacga cacgatcacc
    12361 gaggacgggc gcctttccga ggagtcctac tcggccctct ggcgccttgc ccgggcgggg
    12421 atcgcggccg tgcccgtgac gggccgtccc gccggctggt gcgacctgat cgcccggcag
    12481 tggcccgtgg acgccgtggt cggggagaac ggggccttcg ccttctatat ggaggacggc
    12541 cggctcgagc atctcttcca tcccgaggcc cgggatcccg ggtccgcccg ggcccgcctg
    12601 gagcccctgc gggacctcgt cctctcggac gtggccgggg cccgggtggc caaggaccag
    12661 ttctcccggc tcttcgacct ggcgatcgac ttcgcggagg aggagcccgt cctgcccctg
    12721 gaagccgccg tgcggatccg ggacctctgc gagtccctag gcgctcgggc caagatctcc
    12781 tccatccacg tgaatgcctg gttcggggcc tacgacaagc tgtccatggc cgagctgtat
    12841 ttagcccgcc gccacggctg ggatccggtg agggacctcg ggcgggtcct gtatttcggg
    12901 gactctccca acgacgaacc catgttcgcc cgcttccccc tctccgtcgg ggtggccaat
    12961 gtccgacggt tcctgcctct gatgaagtcg agaccgacct tcatcaccgc cggatcccac
    13021 ggccggggct tcgcggaggg ggtggacctc ctcctgggcc tgcggggata gggtcggcgg
    13081 cgggcgcgtt ccgggaggtg tcgatcgcga ccggcggttt gacggggcac cggcacggga
    13141 tatactggag gaagcccagc gcaacccgga gcgtatccat gaaaagcgcc ttcaggatca
    13201 ccttcgtcta tctcgcggta gcttcggcat ggatcctgct ctcggaccgg gctatcgccc
    13261 tgtttacgga cgacgtcgcc gccctgaccg cctactcgag cgccaagggg ctcttctacg
    13321 tcacggtcac cagtctgatg ctcttcttca tgatccgcaa ggaaatcgcc gtccggaacg
    13381 acgcgataac caggctgaac aggaccctgg aggtcaaggg cgaactgatc cacgagcttc
    13441 accacaggat cgggaacaat atccaggtga tcctgggcgt cctcgggttg gagacccggg
    13501 agggtgattt ttccgcggag gccaaggacc ggatcgtgca tcggctattg gccatgcgct
    13561 cggtctacga catcgtctac gattacgagg acatgcggga gatctccctt cggaaagtcc
    13621 tcgcggccta cgcgcgtacc gcgtcccgga acgtgcgggt ccgggacgat ttcccggaga
    13681 ccccgctgcc catcgaaacc atggtgaccc tcctcctggt cctggacatc gtactggaag
    13741 ccctccggga atccggatac ggaggaaccg cggagatgtc gagcccggaa gaacgcaccc
    13801 tgaccctgcg gatggacggg tactccggcg acgccggaaa ctttctcggg cggaacgaga
    13861 ccttcgtgcg ggcatatctc aagtccatcc ggggcgagat cgatcttctt cccggcgacc
    13921 ccgcggagat acgtgttcgg tatatgtaaa cggttctctt cccggatcca acaagatgga
    13981 caagaagtag ggaaggatag acgagatgaa tccttcatgc gcccaggtcc ccgtcttgtt
    14041 aatcttgaac catcatgtga atcctgttgg atctatggtt cc
//
LOCUS       DAOTEI010000088        13846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3057_length_13846_cov_5.205786, whole genome shotgun sequence.
ACCESSION   DAOTEI010000088 DAOTEI010000000
VERSION     DAOTEI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3057_length_13846_cov_5.205786"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="P5313_09600"
     CDS             complement(<1..662)
                     /locus_tag="P5313_09600"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRY80659.1"
                     /translation="MKKTLERFGKAAAFAALAAAAVPPLTAGEDALTIIREVDRRQRV
                     DTSLTRTAMRIYPDGGNETEYREFVIEGYERGDSESYREVLEPRSVKGLKILSVGDDA
                     WLFFPSSGRVRKIAGASRGESVQGIGGDFSYEDLGGGRFEEKYEFRILASEPRSWTLE
                     GTPRKPDGVYSRLVIRVAREGYRTESIEYHTAKDGRLKTLEFLEYRTLGGVVLDALRA
                     VPL"
     gene            complement(659..3433)
                     /locus_tag="P5313_09605"
     CDS             complement(659..3433)
                     /locus_tag="P5313_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRY80660.1"
                     /translation="MKFESGLSRLGAWTVRHPLGILAATAVLTAAAIWAAAGLGVETD
                     ISQMLPPENPAARGFSEVSEAFATTSSLVVVVEGPDRAALIGAAEAFTARIREDPRTS
                     DLVGSARLKADRAFLETWGLLLQDAEELEDTDRLMESTRLVPLLRAANDLMEEKLSDG
                     SDEEVEGPEGEDEAFALMSRLGLFAEILDRRVAAEAVGTAPDELATDAGLADAWLFGE
                     EYFLDPEERTLLLTLRPTFDLGDRKRLTSLTENAEALAREVESGLPGIRFSFTGDVAN
                     EADEERAIGSDLFYPSLLALSLILILFLASFRRRRSILFATAALGVGIVADLGFAALT
                     VGKLNMITTSFGALLVGLGIDFGIHLASRYDSLSDEIADPAERMGRVFASVGNPVLVG
                     GLTTALAFYSLLLSRTLAFRQFGLIAGTGILTTLAASFFVLPALVAAFPGRPGSGRAR
                     PVLSFSLPVRAARACGRRPGLVLACGAALAVLAGSNIPRNAFDFDMRRIGPQGTAAQE
                     AERLVAERFGISTWQHMALAESLEEARALQERFRDAPLVRGTESAADWIPGAAEQEER
                     LARIALIAARPSRDSGPGWDDKGVEDLLHEVRRLEWNVLELGDLAAMSLGEESLPVRK
                     RNALVREITDGRAGTPGREVFGVLRERVEALPRARAAEILSGIDRGFSAALDRKVRFL
                     AAADRALTVEDLPEDLRSEYVSPDGSRYLVLIRGASGLAESAAIERFAAGLSAVDPEA
                     TGSLALGVELSREILAEARSSALIVGILVFAVVAAGFRSLSLSVLAGAAFAAALAWTF
                     GLQPLFGRFNIVNALSLPLILGVGIDYCVHVLSALRTGNPEDLVHSIKAVTLSMLTTA
                     LGFGSLALAGRFAGIAALGETLTLGILSCYAAALTLVPALAGIPFHRASARRIPAGRP
                     GMVPERKVSK"
     gene            complement(3501..4136)
                     /locus_tag="P5313_09610"
     CDS             complement(3501..4136)
                     /locus_tag="P5313_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY80661.1"
                     /translation="MERRTNTDDNELTARRRILDAAAREFAASGFDGARVDEIARKAG
                     VNKALIYYYFKSKEELLAVLFEETRDAVFALFDAGVIRKMDFGTGDDARRMLTTFLEV
                     LEERQDVIRVVLMECAKRTPINDMIFSMIGEILDRMMALAADTGYLVEKDREKAKVTE
                     FFTGMMPILNYVAYHEIWMERFGMEEPALRAQFIDAFLGTHLAYTVRKAGR"
     gene            4251..4739
                     /locus_tag="P5313_09615"
     CDS             4251..4739
                     /locus_tag="P5313_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRY80662.1"
                     /translation="MNDTDRRLLALAVERARKARAEGNHPFGAVLADAAGRILLDAGN
                     TVVTGRDCTGHAETNLARAASAAYDRDILRTCTLYTSAEPCAMCAGAIYWSGIGRVVY
                     ALSESDLRGLTGDDPENPTLDLPCREVFARGQRPVAVEGPGDAPGAAEVHEGFWKPDT
                     KA"
     gene            4773..7046
                     /locus_tag="P5313_09620"
     CDS             4773..7046
                     /locus_tag="P5313_09620"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRY80663.1"
                     /translation="MNNHVLFDIKLNRPELPAGFLPRKGLEARLEEACSKDFILVSGP
                     AGYGKSLAVSAWARTTGRAVLWFSLGPEDSSLASLASLLSYGLSRMFAGDPGSPLPEG
                     PDSGAVGVTDQAPRYLAALLARQVRERLSDRELVLVLDDYHLCDGEANNSFILELSNQ
                     SSSRMKILISGRSDPGLPISRLRAQNRIDEIRIDDLRFSAVDLAAFLAAHVDRPVPEE
                     LQSLIDRKTEGWPAGLQMLCLAMGRAPEIEDFVRDFSASHRFILDYLMEEVLAGLDEE
                     WIGFLYRTSIAEPFSAGLCAELCGIPDAEDRLRRLDRMNLFLIKLDGDGHWYRYHHLF
                     RDLLRMRAQKALEHDLPGLHGAASRWYRKEGLRELAFLQLMEGGFTEECFRFLEEESR
                     DLYIKGGIPSLLGLYERLPFEAFQSHPEACVWFGWLLASTGKVRQVPALVAWAEEAVR
                     RQPDEGIRNRQSAGLASIKAMTANRNGNFAAAESEAAKAKELFPEGAFRDRAVASYIR
                     ATALILGGRLSDADRELEDSHRLATAGGDTYTQGMALSERARLRILEGSPKEALTLLA
                     RAEVLANPGGAPALWCGMVYIYQALAYRASGDDDKALSCALRGVDLARLRGNATPLVL
                     GYRLLFDLYRESGQTGRCGELLASMEGLLEEFHLYPDSVRVIEEARSIYAERIRKDAR
                     GPGPASERVDGLLSRREAEVLELLALGCSNQEIADRLFLSVGTVKTHLHNISEKLGAA
                     NRTETVALARRHGYLKS"
     gene            7142..7864
                     /locus_tag="P5313_09625"
     CDS             7142..7864
                     /locus_tag="P5313_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY80664.1"
                     /translation="MEARNYEVRVSGELDEIWKAYFAPFVYSRAGAGETVFTGTVRDQ
                     SELIEALDRFQSVNVPILSVTAIQRSHEMSRKPLKPFGGIVPVFAPVFFVLLAAAVLG
                     TSVFREFFRIPFLPEPLRLGLGAAIALVMIPFYASTVRVFLADFKTGQVVTRGTYAYC
                     RNPIYATWIVFLLPAAALLADSWLLLALDLVLYGIFKILIPREYAECREAFGEDYVRY
                     ENSVSEIFPAFWKPYRQEDARE"
     gene            7908..9161
                     /locus_tag="P5313_09630"
     CDS             7908..9161
                     /locus_tag="P5313_09630"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY80665.1"
                     /translation="MLTSISVLFGMGCSTVPNKDGSPAQGFVAPGYEEVRQEFERNIR
                     GRGELGGAFAAYVDGQLVVDLWGGWADPKRTRPWNRDTSVVVFSATKGVGAMTLAHLH
                     SKGLLDYDRKVSDYWPEFAAKGKADITVRQLLSHQAGIVLTPEPLDARNPGEAARILA
                     DTEPLWTPGDYHGYHAGTVGFALQALVLRIDPRGRSINRYLQEELAEPLGADFTIGLT
                     PGYDRSRIAVIKPFSPMEALFHLGEMPKGLRKVVFDGKSLFWKSMGEGKVDVNSTEYL
                     SGENPSGNGIGTARGLAALYGEFASGGRRIGLAPETLAEVFGPAETPRLDRLDRVMNL
                     DSYFGLGFQKPNPADGWFSQNPVAIGFLGASGAIGVADPAHRVGFAYVTNRMSPGTQV
                     NDPRERALQDALYRALRTKMQEGSK"
     gene            9158..9733
                     /locus_tag="P5313_09635"
     CDS             9158..9733
                     /locus_tag="P5313_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRY80666.1"
                     /translation="MKVVAFNGSPREEGNTHFLIEEAAKVLRSRGIEVEEIRVGGRAL
                     RGCNVCFSCKEKKDGRCAIDTDPVNDWIARMIEADGIILGSPSYFADLSPELKALIDR
                     SGFVTRAGGGLLRRKVGAAVVAMRRAGACHAFDSINHFFLSSQMVVAGSNALNIGIGG
                     EEGAVLQDAEGLANLRSLGENMAWLLEKIRS"
     gene            complement(9800..10618)
                     /locus_tag="P5313_09640"
     CDS             complement(9800..10618)
                     /locus_tag="P5313_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY80667.1"
                     /translation="MSILINEMWPGAAILGSVLLAAACAGGPGSATRKDAEARAEQEA
                     ADRSLAGTLMASVPGRTMISRVNRDALYQTSFLIVSKSGTVIVADPYQVLPGIEADIV
                     ISTHKDHDHNDPEFYSRTKARTSRYVLESFTVKDVSIYTVAASHYGDDFDPAAPTDVI
                     YLLDLDDLRIAVMGDIAQTRLTDAQLSAMGAVDVAIVIMELAPKYGYSLENTRTMLDQ
                     LRPRLAFPIHPSEEVVRGVAEAMGGLENASWRYVIDPETLRDESARVVTLAPLP"
     gene            complement(10927..>13846)
                     /gene="nifJ"
                     /locus_tag="P5313_09645"
     CDS             complement(10927..>13846)
                     /gene="nifJ"
                     /locus_tag="P5313_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRY80668.1"
                     /translation="ALRGSSQNPDVYFTSRETLNKLYAAVPGLVQKYMDKLGKITGRP
                     YKLFDYVGAPDAKQVIIAMGSGVEPIEETVEYLVKKGQKVGLLKVRLFRPFSVEHFIR
                     ALPATVQAIAVLDRTKEPGSIGEPLYEDVRTAIGEAQAAGTAPFKKYPKTIGGRYGLG
                     SAEFTPAMVKAVFDELKKAEPKNHFTVGINDDVSGTSLVVDNSFTLDAQGTVECVFIG
                     LGSDGTVGANKNSIKIIGDETDYFAQGYFVYDSKKAGTSTVSHLRFGPKPIRRPYLIS
                     KADFVALHKFSYIEKLDVLAYAKPGGVFLLNSPFAADKVWDHIPVEVQKTIIEKKLKF
                     YVIDGMDIAERAGMGNRVNTVMQTAFFKISGVLPTAEAIKLVKKYTEKTYARKGADVV
                     KKNLDAIDLALKEVHEVKYPAKTAGKLKMTPPVPASAPKFVKEVLGEIIAGRGAALKV
                     SQMPADGTFPTGTTKFEKRNIAEKIPVWAEDLCIQCGNCTMVCPHAVIRLKAYEPKYA
                     VKAPATFKAVDAKGKEFAGMKATLQVAPEDCTGCGACVNICPVLDKANPGRKAINLEP
                     QLPLREAEAANWEHFLKIPNTDVKFLNLSTPKGIAMRQPLFEFSGACAGCGETPYIKL
                     MTQLFGDRSVIANATGCSSIYGGNLPTTPYATRDDGRGPAWSNSLFEDAAEFGYGMRL
                     NADKLNAYAREILAEYKGKGIQPGLANKLLFNPQKDDTDIEAQRADVARLKADLAKVK
                     EAWAADLSAVADHLIRRSVWIFGGDGWAYDIGFGGLDHVIASGRNVNLLVMDTEVYSN
                     TGGQMSKATPFGAVAKFAAAGKDIGKKDLGLIAMSYGYVYVASIAMGANMAQSIKAFR
                     EAESYDGPSIIIAYSHCAMHGIDMMKGMNQQKLSVEAGIWPLYRYDPRLVAEGKNPFQ
                     LDSKEPDTSKIDLFMANEVRFKTLRDADKDRADALAAKEKALVERRWKEYKYIAERPF
                     "
BASE COUNT         2230 a         4467 c         4761 g         2388 t
ORIGIN      
        1 aggggtaccg cacggagagc atcgagtacc acaccgccca gggtcctgta ctccaggaac
       61 tcgagggtct tgaggcggcc gtccttggcg gtgtggtact cgatgctctc cgtgcggtac
      121 ccctcgcggg cgacccggat cacgagccga gaatacaccc cgtccggctt ccggggggtg
      181 ccctccaggg tccaggaacg gggctcggag gccaggatcc ggaattcgta cttctcctcg
      241 aagcggccgc ccccgaggtc ctcgtaggag aagtccccgc cgatcccctg gaccgattcg
      301 ccccgggagg cgccggcgat cttgcgcacc cggccggagg aggggaagaa gagccaggcg
      361 tcgtcgccta cggagaggat cttgagtccc ttgacggacc ggggttccag gacctcccgg
      421 taactctccg agtccccgcg ttcgtagccc tcgatcacga actcccggta ctccgtctcg
      481 tttcccccgt cgggatagat ccgcatcgcc gtgcgggtca ggctcgtgtc gacccgctgg
      541 cggcggtcca cctcgcggat gatggtcagg gcgtcctccc ctgcggtcag gggtgggaca
      601 gcggccgcgg ccagggccgc gaacgcggcg gcctttccga atcgttccag ggtcttcttc
      661 atttcgaaac cttcctttcc gggaccatgc ccggccggcc cgccggtatc cgacgggcgc
      721 tcgctcgatg gaatggaatc ccggccaggg ccgggaccag ggtcagggcg gcggcgtagc
      781 aggacaggat gccgagggtc agggtctccc ccagggccgc gattccggcg aaccggcccg
      841 ccagggccag ggacccgaag cccagggccg tggtgagcat ggagagggtc acggccttga
      901 tagagtgaac caggtcctcg gggttcccgg ttctaagggc ggaaagcacg tggacgcagt
      961 agtcgatccc cacgcccagg atgaggggga gggagagggc gttgacgatg ttgaagcgcc
     1021 cgaagagggg ctggagcccg aaggtccagg ccagggccgc cgcgaaggcc gctccggcca
     1081 ggaccgagag ggagagggac cggaagcccg cggccacgac ggcgaacacg aggatgccca
     1141 cgatcagggc ggaggaccgg gcttcggcca ggatctcccg ggagagctcc acccccaggg
     1201 ccagggagcc ggtggcttcg ggatcgaccg ccgaaagccc cgcggcgaag cgctcgatgg
     1261 ccgcggattc cgcgaggccc gaggcccccc tgatcagcac gaggtatcgg gatccgtcgg
     1321 ggctgacgta ctcggacctg agatcctccg ggaggtcctc gaccgtcagg gcccggtccg
     1381 cggcagccag gaagcggacc ttccggtcca gggccgcgga gaacccccgg tcgattccgg
     1441 aaaggatttc cgcggcccgt gcccggggca gggcttccac ccgttcccgg aggactccga
     1501 acacctcccg gccgggagtt cccgcccggc cgtccgtgat ctcccggacc agggcgttcc
     1561 tcttccgcac cggcagggac tcctccccca gggacatcgc ggccaggtcc cccagttcga
     1621 ggacgttcca ctcgagccgc cggacctcgt ggagcagatc ctcgacgccc ttgtcgtccc
     1681 agcccggccc ggagtcccgg gacggccggg ccgcgatcag ggcgatccgg gcgagccgtt
     1741 cctcctgctc cgccgccccg gggatccagt cggcggcgga ctcggtcccg cgcaccaggg
     1801 gcgcgtcccg gaaccgttcc tggagggccc gagcctcctc caggctttcg gccagtgcca
     1861 tgtgctgcca ggtggagatt ccgaagcgct ccgccaccag cctctccgcc tcctgggccg
     1921 ccgtgccctg gggaccgatg cggcgcatgt cgaagtcgaa ggcgtttcgc gggatgttcg
     1981 agccggccag gacggccagg gccgctccgc aggccaggac gagaccgggt cgcctcccgc
     2041 aggcccgggc cgcccggacg ggcagggaga aggagagcac gggccgggcg cgcccggatc
     2101 cgggccggcc cgggaaggcc gcgacgaggg ccggcagcac gaagaacgac gcggccaggg
     2161 tcgtgaggat gcccgtcccc gcgatcaggc cgaattgacg gaaggcgagg gtccgggaca
     2221 ggagcaggga atagaaggcc agggcggtgg tcaggcctcc gaccaggacc gggttgccca
     2281 ccgaggcgaa gaccctgccc atccgctctg cgggatcggc tatctcatcg gacagggaat
     2341 cgtaccggga ggccaggtgg atgccgaagt cgatgcccag gcccacaaga agggcgccga
     2401 aggaggtggt gatcatgttg agcttgccca ccgtcagggc cgcgaagccc aggtccgcga
     2461 cgatgccgac ccccagggcc gccgtggcga agaggatgga ccggcgccgc cggaaagaag
     2521 ccaggaagag gatcaggatc agggacaacg ccaggaggct cgggtagaac aggtccgagc
     2581 cgatggcccg ttcctcgtcg gcctcgttgg ccacgtcccc ggtgaaggaa aagcggattc
     2641 ccggcaggcc ggattccacc tcccgggcca gggcttccgc gttctccgtg agggaggtca
     2701 ggcgtttccg gtcccccagg tcgaaggtcg gccgcagggt gaggagcagg gtcctctcct
     2761 cggggtccag gaagtactcc tcgccgaaga gccaggcgtc cgccagcccc gcgtccgtgg
     2821 cgagctcgtc cggagccgtt ccgacggcct ccgcggccac gcgacggtcg aggatctccg
     2881 cgaagagccc gagccgggac atgagggcga aggcctcgtc ctcgccctcc gggccttcca
     2941 cctcctcgtc ggagccgtcg gagagcttct cctccatcag gtcgttggcc gcacggagga
     3001 ggggcacgag acgggtggat tccatgaggc ggtccgtgtc ctccagctcc tcggcgtcct
     3061 ggagcaggag cccccacgtc tccaggaagg cccggtccgc cttgagccgc gccgagccga
     3121 ccaggtccga ggtccgggga tcctcccgga tccgcgcggt gaaggcctcc gccgccccga
     3181 tcagggcagc ccggtccgga ccctcgacca cgaccaccag ggaggaggtg gtggcgaagg
     3241 cctcggatac ttccgagaaa cctcgggcgg ccgggttctc cggcgggagc atttgggaaa
     3301 tatccgtttc cacgccgagc cccgcggcgg cccaaatcgc ggccgcggtc agcacggccg
     3361 tggcagccag gatgcccaga gggtgccgta cggtccaggc tcccaggcgg cttagtcccg
     3421 attcgaattt catgatgtcc tccgttaatc taacaggtta gttaaaacta tccggttagt
     3481 taggtcgctt tccaaaaaga tcatcttccg gccttccgca ccgtatacgc caggtgggta
     3541 cccaggaagg cgtcgatgaa ctgggcccgc agggcgggct cctccatccc gaagcgctcc
     3601 atccagatct cgtggtaggc cacgtagttc aggatgggca tcatacccgt gaagaattcc
     3661 gtcaccttgg ccttctcccg gtccttctcg accaggtatc ccgtgtccgc ggccagggcc
     3721 atcatccggt ccaggatctc cccgatcatg gaaaagatca tgtcgttgat gggagtccgc
     3781 ttggcgcact ccatcaggac cacccggatg acatcctgcc gctcctccag gacctccagg
     3841 aaggtggtca gcatcctccg tgcgtcgtct ccggtgccga aatccatttt ccggatgacc
     3901 cccgcgtcga agagggcgaa gaccgcgtcc cgggtctcct cgaagaggac ggccaggagc
     3961 tcctccttgc tcttgaagta gtagtagatg agggccttgt tcacccccgc cttccgggcg
     4021 atttcgtcca cccgggcccc gtcgaacccc gacgccgcga attcccgggc cgccgcgtcc
     4081 aggatgcgcc tgcgcgcggt gagttcgttg tcgtcggtgt ttgtccgtcg ttccatcata
     4141 ttaaccagtt agttaataat agaccgatcc tcgagggctg tcaagaaaga ttccgcacca
     4201 cggagagcga aattgtggac aagcccgtgc agtcgggcta ggattccccc atgaacgaca
     4261 cggacaggcg gctcctggcc ctcgcggtgg aacgggcccg gaaggctcgg gccgagggga
     4321 accacccctt cggggctgtc ctcgcggacg ccgccgggcg gatcctgctg gacgcgggca
     4381 acacggtcgt gaccgggcgg gactgcacgg gccacgcgga gaccaacctt gcacgggcgg
     4441 cctcggcggc ctacgaccgg gatatcttac ggacctgcac cctctacacc agcgcggagc
     4501 cctgcgcgat gtgcgccggg gcgatctact ggtccggaat cggccgggtg gtctacgccc
     4561 tgtccgagtc ggacctccgg ggcctgaccg gggacgatcc ggagaacccc accctggacc
     4621 tgccctgccg ggaggtcttc gcccggggcc agcgccccgt ggccgtggag ggacccgggg
     4681 atgcgcccgg ggccgcggag gtgcacgaag ggttctggaa gcccgacacg aaagcgtagc
     4741 ccggatcgcg gaacgtcggc ccgggggtga gcatgaacaa ccatgtcctg ttcgacatca
     4801 agctgaatcg acctgaactc cctgccggtt tcctaccccg caagggactc gaagcgcgtc
     4861 ttgaggaagc ctgctccaag gatttcatcc ttgtctccgg gcccgcgggg tacggcaagt
     4921 ccctggcggt ctccgcctgg gcgcgcacca cgggacgagc cgttctctgg ttctccctgg
     4981 ggccggaaga ctcctccctc gcatccctgg cttccctgct ttcctacggc ctttcccgca
     5041 tgtttgcggg cgatccgggc tcgcctctcc cggaagggcc ggattcgggg gcggtcggcg
     5101 tgacggatca ggcgccccga taccttgccg ccctcctggc ccggcaggtg cgggaacgcc
     5161 tgtcggaccg ggagctcgtc ctggtcctgg atgactacca cctctgcgac ggcgaggcca
     5221 acaactcctt catcctggaa ctttcgaacc agtcgtcctc ccggatgaag atcctgatca
     5281 gcggcagatc ggacccggga cttccgatct cgaggctgcg cgcccagaac cggatcgacg
     5341 agatccggat cgacgacctg aggttctcgg cggtcgacct ggccgccttc ctggcggccc
     5401 acgtggaccg tcccgtcccg gaggagctcc agtccctgat cgaccgcaag accgagggct
     5461 ggccagcggg cctccagatg ctgtgcctgg ccatgggacg cgctcccgag atcgaggact
     5521 tcgtccggga cttttccgcg agccaccggt tcatcctgga ttacctcatg gaggaggtac
     5581 tggccggatt ggacgaggaa tggatcggct ttctgtacag gacttccatc gcggagccct
     5641 tttccgcggg attgtgcgcg gagctctgcg gaatcccgga cgccgaggac cggctccgcc
     5701 ggctggaccg catgaacctc ttcctgatca agctggacgg ggacggtcac tggtaccggt
     5761 accaccacct gttccgggac ctcctcagga tgcgggcgca aaaggccctg gagcacgacc
     5821 tgcccggact gcacggcgcc gcgtcccgct ggtaccggaa ggagggccta cgggaactcg
     5881 ccttcctcca actgatggag ggtggcttca cggaggagtg cttccggttc ctggaggagg
     5941 aaagccggga cctgtacatc aaggggggaa tcccttccct cctcggacta tacgaacggc
     6001 tcccctttga ggcgttccag tcccatccgg aagcctgcgt ctggttcggg tggctcctgg
     6061 cctcgacggg caaggtccgg caggtgccgg ccctggtcgc ctgggcggag gaggcggtac
     6121 ggcggcaacc cgatgagggg atccggaacc gtcagagcgc gggcctagcc tccatcaagg
     6181 ccatgacggc caatcggaac gggaatttcg cggcggcgga atcggaggcg gccaaggcga
     6241 aggagctctt cccggaaggg gccttccggg accgggccgt cgcctcctac atccgggcca
     6301 ccgcccttat cctgggcggg aggctgtccg atgcggacag ggaactcgag gattcccacc
     6361 gcctggcgac ggcgggaggc gacacgtata cccaggggat ggccctgagc gagcgggcga
     6421 ggctccggat tctcgaaggc agcccgaagg aggccctgac gctgctggct cgggcggagg
     6481 tcctggccaa tcccggcggg gccccggccc tgtggtgcgg catggtgtac atctaccagg
     6541 ccctggccta tcgagcctcg ggagacgacg acaaggctct gtcctgcgcc ctccggggcg
     6601 tggaccttgc ccggctccgg ggcaacgcga cgcccctggt cctgggatac cgcctgcttt
     6661 tcgatctgta tcgggagtcg ggccagacgg gccgatgcgg ggaattgctg gcctccatgg
     6721 agggactgct cgaggagttt cacctctacc ccgacagcgt tcgggtcatc gaggaagccc
     6781 ggagcatcta cgcggagcgt atccggaagg acgcgcgggg acccggcccg gcatcggagc
     6841 gggtcgacgg actcctctcc cggcgcgagg cggaggttct cgagctcctc gccctgggct
     6901 gctcgaacca ggagatcgcg gaccgcctgt tcctgagcgt aggcaccgtc aagacgcacc
     6961 tgcacaacat atccgagaag ctcggggccg cgaaccgcac ggaaaccgtc gccctggctc
     7021 ggcgccatgg gtacctaaag agctgaccct cccggccgct aaacccgccg ctcggtccga
     7081 tgcgcaaaaa cccatccttc gatgtatttt tagggcccgg gtttagctgt acggtacagc
     7141 catggaagca aggaactacg aagtccgggt ctccggagag ctggatgaaa tctggaaggc
     7201 gtacttcgcg cccttcgtct actcccgtgc cggcgcgggg gagacggtgt tcaccggaac
     7261 cgttcgggac caatccgagc tgatcgaagc cctggaccgg ttccaatcgg tcaacgtgcc
     7321 gatcctgtcc gtcaccgcga tacaaaggag tcatgagatg agccgcaaac ccttgaagcc
     7381 cttcgggggc atcgtgcccg tgttcgcgcc cgtgtttttc gtcctgctgg cggccgccgt
     7441 cctcgggaca tcggtattcc gggagttttt ccggattccg tttctgccgg agcctctgcg
     7501 cctggggctg ggggcggcca tcgcccttgt catgatcccc ttctacgcca gcaccgtccg
     7561 ggtcttcctt gcggacttca agaccggcca ggtcgtgacc cggggcacct acgcgtattg
     7621 tcgaaacccg atctatgcca cctggatcgt gttcctcctg cccgcggcgg ccctcctggc
     7681 ggactcctgg ctccttctgg ctctggacct tgtcctgtac gggattttca agatcctgat
     7741 cccccgggaa tacgcggagt gccgggaggc attcggggaa gactatgtcc ggtacgagaa
     7801 cagcgtgagc gagatttttc cggccttctg gaagccctac cgtcaggagg acgcccgtga
     7861 gtgatcggat agaatcgctt cggatcgcgg gaataaggat ggccgtgatg ctgacttcga
     7921 tctcggttct ttttgggatg ggctgttcga cggttccgaa caaggacggg agccccgcgc
     7981 aaggcttcgt cgcacccggg tacgaggaag tccgccagga gttcgagagg aacatccggg
     8041 gccggggaga actgggcgga gccttcgccg cctacgtcga cggacagctc gtggtcgacc
     8101 tctggggggg ctgggcggac ccgaaacgga cccggccctg gaatagggac acatccgtcg
     8161 tggtgttctc cgccacgaag ggggtgggcg ccatgaccct ggcccacctc cactcgaaag
     8221 gacttctgga ctacgaccgg aaggtttccg actattggcc ggagttcgcg gccaagggca
     8281 aggcggacat caccgtgcgc cagctccttt cccaccaggc cgggatcgtc ctgactccgg
     8341 aacccctgga cgcccggaat cccggggagg cggcgcggat cctggcggac acggagcccc
     8401 tctggacccc cggggactac cacggctacc acgcgggaac cgtcggattc gccctgcagg
     8461 ccctggtgct tcggatcgac ccccggggcc gcagcatcaa ccgctacctc caggaagagt
     8521 tggcggagcc cctgggcgcg gacttcacga tcgggctgac cccggggtac gaccggagcc
     8581 ggatcgcggt catcaaaccc ttcagcccga tggaagcgct gttccacctg ggcgagatgc
     8641 ccaaggggct tcgcaaggtg gtattcgacg ggaagagcct tttctggaaa tccatggggg
     8701 agggcaaggt cgacgtgaac tccacggagt acctttccgg agagaatccc tcggggaacg
     8761 gcataggcac ggctcggggc ctggcggccc tgtacggcga gttcgcctcc ggcggcaggc
     8821 ggatcggcct tgcgccggaa accctggcgg aggtcttcgg ccccgcggag accccgaggc
     8881 tggaccggct ggatagggtc atgaacctgg actcctactt cggcctgggc ttccagaagc
     8941 cgaatcccgc ggacggatgg ttcagccaga atcctgtcgc cataggcttt ctgggcgcct
     9001 ccggggcgat cggggtcgcg gatcccgcgc accgggtcgg gttcgcctac gtgacgaacc
     9061 ggatgtcccc gggaacccag gtgaacgatc cccgggagcg ggcacttcag gacgccctct
     9121 acagggcgct tcgaaccaag atgcaggaag gttccaaatg aaggtcgtgg cgttcaacgg
     9181 aagtccccgg gaggagggga atacccactt cctgatcgag gaggccgcca aggtactccg
     9241 gtcccggggt atcgaggtcg aggagatccg cgtcggaggc cgggccctcc ggggctgcaa
     9301 cgtctgtttc tcctgcaagg aaaagaagga cggccgctgc gccatcgaca ccgatcccgt
     9361 caacgattgg atcgctcgga tgatagaggc cgacgggatc atcctgggct ctccctcgta
     9421 tttcgcggac ctgagcccgg agctgaaggc cctcatcgac cggtccggtt tcgtcacccg
     9481 cgcgggcggg ggacttttaa ggcggaaggt cggggccgcc gtagtggcca tgcgccgcgc
     9541 gggggcctgc cacgccttcg attccatcaa ccacttcttc ctttcctccc agatggtggt
     9601 cgccggctcc aacgccctga acatcgggat cggaggcgag gaaggggcgg tgctccagga
     9661 cgcggagggc ctggcgaacc tgcgttccct gggggagaac atggcttggc tgctggagaa
     9721 gattcgttca tgaccgcggc cgtcgaagtt tggcggggcc cctcacagcg ggagggtccc
     9781 gtcgggacta cagaggaggt tacggcagcg gcgccagggt cacgactctg gcggattcat
     9841 ccctaagggt ttccggatcg atcacgtagc gccaagatgc gttttctagc ccgcccatgg
     9901 cttcggcgac gccccggaca acctcctccg aaggatggat cgggaacgcg agtctcgggc
     9961 gaagttggtc caacatcgtc cgggtgttct ccaggctgta gccgtacttc ggggcaagct
    10021 ccatgatcac gatcgccacg tccaccgccc ccatcgccga aagttgggcg tccgtaagcc
    10081 gggtctgggc gatgtccccc atgaccgcta tccggaggtc gtccaggtcg aggaggtaga
    10141 tgacgtcggt cggggcggcc ggatcgaaat cgtccccgta atgcgaggcg gccaccgtgt
    10201 agatcgagac gtctttcacc gtgaaggatt ccaggacgta gcgggacgtg cgggccttcg
    10261 tccgggagta gaactccggg tcattgtggt catggtcctt gtgggtggag atgacgatgt
    10321 cggcctcgat ccccggaagg acctggtacg gatccgcgac gatcacagtg ccggatttcg
    10381 agacgatcag gaaactcgtc tggtacaggg cgtcccgatt aacccggctg atcatcgttc
    10441 gtcccgggac gctggccatc agcgtcccgg ccaggctgcg gtcggcggcc tcttgctccg
    10501 cccgggcttc ggcgtccttt ctcgtggcgc taccgggccc gcctgcgcag gccgcggcga
    10561 gcagtaccga acccaggatg gcggccccgg gccacatctc gtttataagg atcgacatgg
    10621 cggttccttt tctggattct gtcggatcag ggcattgtcg aaccgggagg tttttctgtc
    10681 aagaatggac ctgcgattga tgagtgggtg cacgtgaaac cgcttcgcgg tttcacggcg
    10741 acgaagctgc gcttcgtcga tcgcccggaa ccgttcgccc aaggcggcgc acctgcggaa
    10801 gtcggcggga agcgccggat atggaaccat gcacggccct gggttccaca gggccgcggg
    10861 cgctcctccc acaagcaacg gccgccccgt agggggcggc cgtttgatca ctagatcgtc
    10921 aatggtttag aacggccgct cggcgatgta cttgtattcc ttccaccggc gctcgaccag
    10981 ggccttctcc ttcgcggcca gggcgtccgc gcggtccttg tcggcgtccc ggagggtctt
    11041 gaaccgcacc tcgttggcca tgaacaggtc gatcttggag gtgtcgggct ccttggagtc
    11101 cagctggaag ggattcttgc cctccgcgac caggcgcgga tcgtagcggt agagcggcca
    11161 gatcccggct tccacggaga gcttctgctg gttcatgccc ttcatcatgt cgatcccgtg
    11221 catggcgcag tgggagtagg cgatgatgat ggacgggccg tcgtagctct ccgcctcccg
    11281 gaaggccttg atggactggg ccatgttggc gcccatggcg atgctggcca cgtacacgta
    11341 gccgtagctc atggcgatca gacctaagtc cttcttgccg atgtccttgc ccgccgcggc
    11401 gaacttggcc accgcgccga agggcgtggc cttggacatc tggccgccgg tgttggagta
    11461 gacctcggtg tccatgacca ggaggttcac gttccggccg gaggcgatca catggtccag
    11521 gccgccgaag ccgatgtcgt aggcccagcc gtcgccgccg aagatccaca cggagcggcg
    11581 gatcaggtgg tccgccaccg cggagaggtc cgcggcccag gcttccttga ccttggccag
    11641 gtcggccttg agccgggcca cgtccgcccg ctgggcctcg atgtccgtgt cgtccttctg
    11701 ggggttgaag aggagcttgt tggccaggcc gggctggatg cccttgccct tgtactcggc
    11761 caggatctcc cgggcgtagg cgttcagctt gtccgcgttc aggcgcatgc cgtacccgaa
    11821 ctccgcggcg tcctcgaaga gggagttgga ccaggccggt ccgcgtccgt cgtcccgggt
    11881 cgcgtaggga gtggtgggca ggttgccgcc gtagatggag gagcagcccg tggcgttggc
    11941 gatgacggac cggtccccga agagctgggt catcagcttg atgtacgggg tctctccgca
    12001 gcccgcgcag gcgccggaga actcgaacag gggctggcgc atggcgatgc ccttgggggt
    12061 ggacaggttg aggaacttga cgtccgtgtt ggggatcttg aggaagtgct cccagttggc
    12121 ggcctcggcc tcccgcaggg gcagctgggg ctccaggttg atggccttgc gcccggggtt
    12181 ggccttgtcc aggaccgggc agatgttcac gcaggcgccg cagccggtgc agtcctcggg
    12241 ggccacctgg agggtggcct tcatacccgc gaactccttg cccttggcgt ccacggcctt
    12301 gaaggtggcc ggggccttga cggcgtactt gggctcgtag gccttgaggc ggatgaccgc
    12361 gtgggggcag accatggtgc agttgccgca ctggatgcag agatcctcgg cccagacggg
    12421 gatcttctcc gcgatgttcc gcttctcgaa cttggtggtc ccggtgggga aggtcccgtc
    12481 cgcgggcatc tggctgacct tgagggccgc gccgcggccg gcgatgatct cgcccaggac
    12541 ttccttgacg aacttgggcg cgctcgccgg gaccgggggg gtcatcttga gcttgcccgc
    12601 ggtcttggcc gggtacttga cctcgtggac ttccttgagg gccaggtcga tggcgtccag
    12661 gtttttcttg accacgtccg cgcccttccg ggcataggtc ttctccgtgt acttcttgac
    12721 cagcttgatg gcctccgcgg tggggaggac gccggagatc ttgaagaagg cggtctgcat
    12781 gaccgtgttg acccggttgc ccatgcccgc acgctcggcg atgtccatcc cgtcgatcac
    12841 gtagaacttg agcttcttct cgatgatggt cttctggacc tccacgggga tgtggtccca
    12901 gaccttgtcc gcggcgaagg gggagttgag caggaagacc ccgccgggct tggcgtacgc
    12961 caggacgtcc agtttctcga tgtagctgaa cttgtggagg gccacgaagt cggccttgga
    13021 gatcaggtag ggcctgcgga tgggcttggg cccgaagcgc aggtggctga ccgtggaggt
    13081 gccggccttc ttggagtcgt acacgaagta gccctgcgcg aagtagtccg tctcgtcccc
    13141 gatgatcttg atggagttct tgttggcccc cacggttccg tcggaaccga ggccgatgaa
    13201 cacgcactcc acggtgccct gggcgtccag ggtgaaggag ttgtccacca cgagggaggt
    13261 gccggacacg tcgtcgttga tgcccacggt gaaatggttc ttcggctcgg ccttcttgag
    13321 ctcgtcgaag acggccttga ccatggcggg ggtgaactcc gcggacccga ggccgtagcg
    13381 gcccccgatg gtcttggggt acttcttgaa gggggccgtg ccggcggcct gggcttcccc
    13441 gatggcggtg cggacgtcct cgtagagggg ctcgccgatg gatccgggct ccttggtccg
    13501 gtccaggacg gcgatggcct ggacggtggc gggcagggcc cggatgaagt gctcgacgct
    13561 gaaggggcgg aagagccgga ccttgagcag gccgaccttc tggccctttt tgaccaggta
    13621 ctcgacggtc tcctcgatgg gctccacgcc ggatcccatg gcgatgatga cctgcttggc
    13681 gtccggggcg cccacgtagt cgaagagctt gtagggccgg ccggtgatct tgcccagctt
    13741 gtccatgtac ttctggacca ggccggggac cgcggcgtag agcttgttca gggtttcccg
    13801 gctggtgaag tagacgtccg ggttctggct ggagcctcgg agggcg
//
LOCUS       DAOTEI010000089        13561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3151_length_13561_cov_4.457204, whole genome shotgun sequence.
ACCESSION   DAOTEI010000089 DAOTEI010000000
VERSION     DAOTEI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13561
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_13561_cov_4.457204"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /gene="nadA"
                     /locus_tag="P5313_09650"
     CDS             complement(<1..765)
                     /gene="nadA"
                     /locus_tag="P5313_09650"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRY80669.1"
                     /translation="MVLYRIMIEDSSAGPSALSVPDETALADLARLKKERGALVLAHT
                     YQLPEVQAAADLVGDSLELSRAAARTDARVIVFCGVRFMAETAKILAPDRTVLLPVES
                     AGCPMADSITAEDLRAFKSRHPGRPVVTYVNSTAEVKAESDWCCTSANALKVIEAVDA
                     PEILCVPDRNLARWCQRNTKKKLIWWDGFCMVHVRITPEDARAGKAKYPDALLLAHPE
                     CEPEVLDLADHVLSTSQMLAFAARSPARRFLIATEAE"
     gene            862..1923
                     /locus_tag="P5313_09655"
     CDS             862..1923
                     /locus_tag="P5313_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80670.1"
                     /translation="MKPVLALLCLLSLMPVPFHGQVPARADFSSLADTHEARTRYLTS
                     VITANWNSAVRFPETFLTGAAGRVQVSVDRQEDFFYVRFLRERSGAFPLVSQGNTIVQ
                     RNYQRNGDLIQAKIFLSDDPSCYLRLYPQAERTRADIVLFGAVVRQNILLGQIFYYQL
                     RDHVGKMLTAAEGSLDWSIFIRSPGGAPSGAVRFGQALSAGRLTPGGPGFRLISSLAY
                     DDSVEEFLSGSGRVLGAGELASVPSHPFADDRDPRRTAAYRSFPRYEPGKGLPAAAAA
                     ALVHSHSLEHPDDLYAAFLQDGPRPRRILLVPGFGPDGIFGIQGYDPETRQNLSWPEF
                     AAEHRELPVRLVRVPAPQL"
     gene            1945..2484
                     /locus_tag="P5313_09660"
     CDS             1945..2484
                     /locus_tag="P5313_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRY80671.1"
                     /translation="MSRAFVDEASADSRDEDAPEFKIPLPPGARNYVTPEGAERLYSE
                     LKTLEATERPDLAAEILRLSRDPTNSDALATARRRLARLDRRIEYLARMAGLAEVVNR
                     PAGGYDRVRFGARVTVRYGDGATSTFRIVGVDESDPEAGLVGWTAPLARALMGRAPGE
                     NSVVLLPGGEKTVTVLAVE"
     gene            2565..3398
                     /locus_tag="P5313_09665"
     CDS             2565..3398
                     /locus_tag="P5313_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRY80672.1"
                     /translation="MKLTYSVCNELFGDLPLPRAARIIRESGYTGIEFAPFTIFGDFS
                     PGSVRDGLAEARDVLDGEGLSFAGFHWLLAKPEGLHLASPDPAVRSRSVDHLRFLADC
                     AGRLGGGPLILGSPRQRGSLPEVPKAEAEAALDRALREAAPAVEAAGCSLLLESLSPE
                     QTDVVNSLAEAVRRARAAGSPAVSSMFDFRNVSGEAEGWQALLDRHFGDIRHVHANEV
                     DGRAPGTGSSDYLPAFRTLLARGYRGWVSVEIFEVPPDPGAILRGSMDLFKRLEEEGS
                     R"
     gene            3395..4441
                     /locus_tag="P5313_09670"
     CDS             3395..4441
                     /locus_tag="P5313_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY80673.1"
                     /translation="MKFGIVGTGMIAEFHARAIGEIPGASVAACLDTVPERARSFAEK
                     HKCRAYGDLKSFLSDPEMEVVNVCSPSGAHLDSAVPAAESGRHLIVEKPLEITVERCL
                     RIVEACSRSGVVLSGIFPSRFFEAARAVKEAVDQGRFGRLSMGSAYVKWWREQAYYSG
                     SGWKGTKAMDGGGALMNQSIHAVDLLLWYMGPVRRVSAFTGVAGHTGLEIEDNASAAL
                     EFENGALGAIQGSTAVWPGFLKRVEVLGTSGSAILEEESLAFWRFASETPADEEARSR
                     FASLTSTGGGASDPSAIGHRGHRLQIEDVLRAIGEKGKPLVDGIEATRPVALIRAIYE
                     SAASGRPVTPADPA"
     gene            4465..5391
                     /locus_tag="P5313_09675"
     CDS             4465..5391
                     /locus_tag="P5313_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRY80674.1"
                     /translation="MKDGTMRLAGFADEAADEVEGQVRVTKALGWKWIEARNSGGVNV
                     HDLPDRDFESFRAALEKAGIGVCCLGSSIANWGKSPDEDFAASLAAARRAADRMEVLG
                     TRLVRIMSYAVLVDPEGNPLPDQKKRQRFQRLRELCGVFTERGMTPVHENCFNYGGMG
                     VENTRELLSEVPGLKLVFDTGNPSLTPDFTRPPPRPNQDSWESWTALRDHVVHIHVKD
                     GWRDPGTGAETYVFPGEGPSRVKDILADCAARGYSGWLTIEPHMAVVYHDPTVSSPAA
                     AREAVYLEYGRRLQTLLASLGLEVRDGEARRP"
     gene            5406..6449
                     /locus_tag="P5313_09680"
     CDS             5406..6449
                     /locus_tag="P5313_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY80675.1"
                     /translation="MDTWKLPSFKTEEELESRMSEPTAAVVEEFRKNPGDLAILGIGG
                     KIGVTLGMMAARARDAAGVRSRILGVSRFSDKAARARLEKAGVECLECDLLDRSSVDK
                     LPDAPRVVFMAGKKFGTSGSEETTWAMNTVVPAYVAGRYRDSAIVAYSTGCVYDVVTP
                     YSGGSSESDPPRAVGDYAQSALGRERVFQHFSRAEGTRVCILRLNYAIDPRYGVLHDI
                     ARKVAAGEGVELAVENFNCIWQGDVLERTLLAFRHAASPAFVLNLTGPETASVRRTAE
                     EFGRLLGKPPVFRGGGEGGRAYLNDAALSFRLFGYPRVPLAEMIRMQADWIASGGGSL
                     GKPTHFETTDGTY"
     gene            6465..7541
                     /locus_tag="P5313_09685"
     CDS             6465..7541
                     /locus_tag="P5313_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY80676.1"
                     /translation="MKRSDIPTGLLETLRRGTVIPAIPLALDADRRFDPRRQKALVRY
                     YIDAGAGGIAAGVHTTQFEIRDPRHGLFRPVLSAVSAAIDEWCGRRGTRVLKVGGVCG
                     PTHQACQEADFLVSAGYHAGLLSLAGHGEDSIEDLLAHVRKVAGRIPVIGFYLQPAVG
                     GRVLPYEFWRGLCEIENVIAIKMAPFNRYRTFDVVRAVAESGRAGEIALYTGNDDNIV
                     VDLLTEYAVRTAGETRRLRIAGGLLGHWSVWTRRAVELLRSARDLSDAGAPIPQSLLT
                     LAQEVTDANAAVFDAANGFAGCIPGIHEVLRRQGLLSGTWCLNPQEGLSPGQAEEIDR
                     VCRSYPHLTDDEFVTAHLPEWLAD"
     gene            complement(7722..9239)
                     /locus_tag="P5313_09690"
     CDS             complement(7722..9239)
                     /locus_tag="P5313_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY80677.1"
                     /translation="MFEGLKLFGEAMGTVFTFRALFDSLWATQLGIIIGMLPGLTATM
                     GVALLTTLTFNMAPGNAILILICMYIGAIYGGSRSAILLNIPGTPANAATTVDGYPLA
                     LQGKAGKAIGIATTGSFLGSMIGMLAMLVFTPMIGTVALSFQSYEFVWFSIFGIIICG
                     NLTAPKDPLKGWISGFLGLFVAMIGMEGIHAYVRFSFGSTNLSGGISLIPAMVGAFGF
                     AEIISVMRVEKYEVVKTAIDRVIPRLSEVFKYWKTIIRSGLIGTFIGAIPGVGEDIAA
                     WVSYDMAKRGAKPEEREKFGKGSVEGLLASETGNNACVPGAIIPVLTLAIPGSAPAAV
                     LLGAMLIHGVRPGPLIMLEFPTFIYEVVAMVLLATIMMFVLGLSMVRPLVKVLQVPRQ
                     KLMPVIFILCVVGSFALQARLFDVGIMIVFGALGFILREMDYPMAPLVLGIILGDILD
                     KNLRRALILSDGSVAPFFTRPICVVLGLLTLIIVLGRAKWFRALMAQMFRKKAAV"
     gene            complement(9250..9762)
                     /locus_tag="P5313_09695"
     CDS             complement(9250..9762)
                     /locus_tag="P5313_09695"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY80678.1"
                     /translation="MTERTMTKADLVTSMLLIVFSAAILLMSLRMPTMADRNASPFSG
                     PGVVPAFVGAVLFLLSLSMLIRSLRKGAPRLFAEDRAGTGTGDRSSWVRIARTLVLCI
                     GYVILLGRIWFPLLTFLFVFLFILTFEYDTKVPLTAQWKKPLFAAILAAVAAASVFLV
                     FQYLFLVNLP"
     gene            complement(9877..10887)
                     /locus_tag="P5313_09700"
     CDS             complement(9877..10887)
                     /locus_tag="P5313_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY80679.1"
                     /translation="MKLSARILAVALVLILAVGAVSAQANWKPNKPITIIVPWGAGGS
                     TDQVTRVCAAELEAALGTKIVIVNQPGASGSVGTKNALDAPKDGYTWTAGAAADLATY
                     KVQGMLDTDIRKDWHVFLSVANVSVVGVNAMTPYKDFSELLAAFKSKPGQITVATAGQ
                     SSAGHIGIELIRKYTGIEYKHVTYDGGNPAVIACVSGETEMTTQLAVEQADMIRGKKI
                     RPLAVMSDKPLNLEGFGEIPPITKWVKDFKTGPNYFGIFIPVGVPKNVVDTVERAWKN
                     VIMKSTKVQEYAKSRGAMFAPSFGKEAQDRAFSYYQPVAWLYWDAGKAKVSPDTVGIP
                     KP"
     gene            11024..11872
                     /locus_tag="P5313_09705"
     CDS             11024..11872
                     /locus_tag="P5313_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HRY80680.1"
                     /translation="MTPSASPRTEPEPARRCPIALVTGSSRGLGRGIALELAAAGFSV
                     AVHYAGNEEAARETLRLCADAAERFRPDRAPEPRFEAFRADLSDPAARAALARDVFSA
                     FGTLDALVNNAGMAPRIRADILDASEESFDELIATNVRGPYFLTRDVARLWLAGPRDT
                     PLPGGRKVVFVTSISAVTASVNRGDYCMSKAALSMAAKLWAARLAPEGIPVYEVRPGI
                     MLTDMTSGVKEKYDALLAQGLVPQGRWGTPEDTGKAVRALLEGAFPFSAGGIFDVDGG
                     FHVSRL"
     gene            11897..13315
                     /locus_tag="P5313_09710"
     CDS             11897..13315
                     /locus_tag="P5313_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRY80681.1"
                     /translation="MIDLRNAPNPPALADACGRAFSAADAKIRALAARWKAGSGAPVF
                     TDAGRYTARGWTEWTQGFQFGMPLLVHEASGDPWHLEYGRSKTLRRMAPHLTHTGVHD
                     HGFNNVSTYGTLLRHAHEGVFPAETWERRYYALALKASGAVQAARWTSLPESLGFIRS
                     FNGPHSLFADTIRSLRSLALGHRLGHVLLGEQDAKANLLGRLLAHAETTARYNVYFGT
                     GRDRWDVRGRVAHESVFNTVNGSYRCASSQQGYSPFTTWTRGLAWIILGYAEELEFAA
                     GLSDAEVAACGLAYFPDAASVRARFLETARAVSDFYIENTPPDGIPYWDTGGPRLREI
                     PGALDRPADPENPFEPVDSSAAAIAAQGLLRLGAFLTAAGDPDGARYTAAGLRTAASV
                     LSAPYLSENPKHEGLLLHAVYHYPNAWDRSADGSPVPHGESCLWGDYHLLELALWISR
                     TARGRGGQRFFDIGLDPDGGGS"
     gene            13312..>13561
                     /locus_tag="P5313_09715"
     CDS             13312..>13561
                     /locus_tag="P5313_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRY80682.1"
                     /translation="MNDLSRLAIHTATTKPWDLETAVRKYADAGVSGISVWKEALEGR
                     DPAAAGAAIRAAGLAPVSLVRGGFFPAPDPETRAVRIRD"
BASE COUNT         2130 a         4635 c         4696 g         2100 t
ORIGIN      
        1 ctcggcctcc gtggcgatca ggaagcgccg ggccggggac cgggccgcga aggccagcat
       61 ctggctggtg gagagcacgt ggtccgccag gtcgaggacc tcgggttcgc attccgggtg
      121 ggccaggagg agggcatccg ggtacttcgc cttcccggcc cgggcatcct cgggggtgat
      181 ccggacatgg accatgcaga aaccgtccca ccagatgagc ttcttcttgg tgttccgctg
      241 gcaccaccgg gccaggttcc ggtccgggac gcacaggatc tccggggcat ccacggcctc
      301 gatgaccttg agggcgttgg cgctggtgca gcaccagtcg ctctcggcct tcacttccgc
      361 ggtggagttc acgtacgtga ccacgggacg gccgggatgg cgggacttga aggcccggag
      421 atcctccgcg gtgatggaat ccgccatggg acaacccgcg gactccacgg gcaggaggac
      481 cgtgcggtcc ggggccagga tcttggccgt ctcggccatg aaccgcacac cgcagaacac
      541 gatgacccgg gcgtccgtcc gggccgcggc ccgggagagc tccagggagt cccccaccag
      601 gtccgccgcg gcctggacct cgggaagctg ataagtgtgg gccaggacga gggctccccg
      661 ctcctttttc aggcgggcga ggtccgcaag ggcggtctcg tcgggcacgg aaagcgccga
      721 agggccggcg ctggaatcct cgatcatgat gcgatagagt accatggacc gcggaggaat
      781 gataagcgct gccgggccgt ctatccttcg ggtccgtcgt atagtatact cgcataccct
      841 caagacaccg gagcggaacc gatgaagcca gtccttgccc tgctttgcct cctatccctg
      901 atgcccgtcc cgttccacgg gcaggtcccg gcaagggccg atttctcgtc cctggcggac
      961 acccacgagg caaggacgcg gtatctgacc tccgtcatca ccgccaactg gaactccgcc
     1021 gtacggttcc cggaaacctt cctgaccggg gccgcgggcc gggtccaggt gagcgtggac
     1081 cgccaggagg acttcttcta cgtccggttc cttcgggagc gctcgggcgc cttccccctg
     1141 gtgtcccagg gaaacacgat cgtccagcgc aattaccagc ggaacgggga cctgatccag
     1201 gccaagatat tcctgtccga cgatccctcc tgctacctcc ggctctaccc ccaggcggaa
     1261 cggacccgtg ccgacatcgt cctcttcggg gccgtggtcc ggcagaacat actcctgggc
     1321 cagatcttct actaccagct tcgggaccat gtcgggaaaa tgctcaccgc cgcggaagga
     1381 tccctggatt ggagcatatt catccggtcc cccggcggcg ccccctccgg cgccgtccgc
     1441 ttcggccagg ccctttccgc cgggcggctg acccccgggg ggcccggctt ccgcctgatc
     1501 tccagcctgg cttacgacga ctcggtcgag gaattcctct ccggttccgg ccgggtcctg
     1561 ggcgccgggg agctcgcctc cgtcccgtcc catcccttcg cggacgaccg ggatccccgg
     1621 cggacggcgg cctacaggtc cttcccccgc tacgaacccg gcaagggact tcccgccgct
     1681 gccgccgccg cgctggtcca ctcccattcc ctggaacacc ccgacgacct gtacgccgcc
     1741 ttcctgcagg acggtccccg tccccggaga atcctcctcg tcccgggctt cggaccggac
     1801 gggatcttcg gcatccaagg gtatgatccc gaaacgcggc aaaacctttc ctggcccgag
     1861 ttcgccgcgg agcatcggga actccccgtc cgcctggtgc gggttcccgc tccccaactc
     1921 taggcgccgg gcgggggtag aagggtgagc cgggccttcg tggacgaggc ctccgcggat
     1981 tcccgggacg aggacgcccc ggagttcaag atccccctgc ccccgggagc ccgcaactac
     2041 gtaacccccg agggagccga gcggctctac tcggaactca agaccctgga agctacggaa
     2101 cgcccggacc tggccgcgga gatcctccgg ctgtcccggg atcctacgaa ctcggacgcc
     2161 ctcgccacgg cccggcggcg cctggcccgg ctggaccggc gcatcgagta cctggcccgc
     2221 atggcgggcc tcgcggaggt ggtgaaccgt ccggccgggg gatacgatcg ggtgcggttc
     2281 ggggcccggg tcaccgtccg gtacggggac ggggccacct cgaccttccg gatcgtgggg
     2341 gtggacgagt ccgatcccga agccgggctc gtggggtgga cggcccccct ggcccgggcc
     2401 ctgatgggca gggcccccgg ggagaactcc gtggtcctcc ttccgggcgg ggagaagact
     2461 gtgaccgtcc tggccgtgga atagccgcga ccggggcgcc ctgccccggc ggaccgccct
     2521 gttttccttg acagcccgcc gggccgccct tagagtgaaa tcccatgaag ctgacgtatt
     2581 ccgtctgcaa tgagcttttc ggggaccttc ccctgcctcg ggcggcccga atcatccggg
     2641 agtccgggta taccggcatc gagttcgcgc ccttcacgat cttcggggac ttctcaccag
     2701 ggtccgtccg ggacggattg gccgaggctc gagacgtcct ggacggggag ggactgtcct
     2761 tcgcgggctt ccactggctc ctggccaagc ccgaggggct ccacctggcc tccccggatc
     2821 ccgcggtccg cagtcggagc gtggaccatc tcaggttcct ggcggactgc gcgggaaggc
     2881 tcggcggagg ccccctgatc ctgggctccc cccgacagcg gggcagcctg cccgaggttc
     2941 cgaaggccga ggcggaggcc gccctggacc gggccctccg ggaggcggcc cccgcggtcg
     3001 aggccgcggg gtgctccctg ctcctggaat ccctgtcccc ggagcagacc gacgtggtga
     3061 actccctggc cgaggccgtg cggcgggccc gggcggcggg aagccccgcg gtgagctcca
     3121 tgttcgactt ccggaacgtc tccggcgagg ccgagggctg gcaggccctg ctggaccggc
     3181 atttcgggga catccggcac gtccacgcca acgaagtcga cggccgggcc ccggggaccg
     3241 ggagttccga ctacctcccg gcctttcgga ccctcctggc ccggggctac cggggctggg
     3301 tcagcgtgga aatcttcgag gttcccccgg acccgggcgc gatcctgaga ggctcgatgg
     3361 acctgttcaa acggctggag gaggagggat cccgatgaag ttcggcatag tcggcacggg
     3421 gatgatcgcg gagttccacg cccgggcgat cggggaaatt ccgggggcgt cggtggcggc
     3481 ctgcctggac acggtgcccg agcgggctcg ctccttcgcg gagaagcaca agtgccgggc
     3541 ctacggtgac ctcaagtcct tcctgtccga tccggagatg gaggtggtca acgtctgttc
     3601 cccctcgggg gcccacctgg attccgccgt accggccgcg gagtcgggcc gccacctgat
     3661 cgtcgagaag ccccttgaga tcaccgtgga gcgctgcctg cgcatcgtgg aagcctgctc
     3721 ccgctcgggg gtcgtccttt cggggatctt cccctcccgc ttcttcgagg ccgcccgggc
     3781 ggtcaaggag gcggtggacc aaggcaggtt cggaaggctc tccatgggca gcgcctacgt
     3841 gaagtggtgg cgggaacagg cctattactc cggcagcgga tggaagggca ccaaggcgat
     3901 ggacgggggc ggggccctca tgaaccagtc catccacgcc gtcgacctcc tgctctggta
     3961 catgggtccc gtccgccggg tcagcgcctt cacgggggtg gcgggccaca cggggctgga
     4021 gatcgaggac aacgcctccg cggccctgga gttcgagaac ggggccctcg gggcgatcca
     4081 gggctccacc gcggtctggc cgggcttcct gaagcgggtc gaggtcctgg gcacctccgg
     4141 gagcgccatc ctggaggagg agagcctggc gttctggcgc ttcgcctccg agacccccgc
     4201 ggacgaggag gccaggtcgc ggttcgcgtc cctgacctcg acggggggcg gggcctcgga
     4261 cccctcggcc atcgggcacc ggggacaccg gctccagata gaggacgtcc tgcgggccat
     4321 cggggagaag ggaaagcccc tggtggacgg aatcgaggcg acccgcccgg tcgcgctgat
     4381 ccgggcgatc tacgagagcg ccgcgtccgg gcggcccgtg acgcccgcgg atcccgcctg
     4441 agcggcctgc ggatggaggt tgaagtgaag gacggaacca tgcgtcttgc gggtttcgcg
     4501 gacgaggcgg cggacgaggt cgaaggccag gtccgggtca cgaaggccct gggctggaag
     4561 tggatcgagg cgcgcaactc gggcggtgtg aacgtccatg acctgccgga ccgggacttc
     4621 gagtccttcc gggcggccct ggagaaggcc gggatcgggg tctgctgcct gggctccagc
     4681 atcgccaact ggggcaagag cccggacgag gacttcgccg cgtccctggc ggccgcccgg
     4741 cgggccgcgg accggatgga ggtcctggga acccggctgg tgcggatcat gagctatgcc
     4801 gtgctggtcg acccggaggg gaatcccctt ccggaccaga agaagcgcca gcggttccag
     4861 cggctccggg aactctgcgg ggtcttcacg gaacggggca tgaccccggt gcacgagaac
     4921 tgcttcaact acggcggcat gggcgtggag aacacccggg aactcctctc cgaggtgccc
     4981 ggcctcaagc tcgtcttcga cacgggcaac ccttccctga ccccggactt cacccggccc
     5041 ccgccccggc ccaaccagga ttcctgggag tcctggacgg ccctgcggga tcacgtggtc
     5101 cacatccacg tcaaggacgg atggagggat cccgggacgg gggcggagac ctacgtcttt
     5161 ccgggggagg gaccgtcccg ggtcaaggac atcctggcgg actgcgcggc ccggggctat
     5221 tccggctggc tcaccatcga gccccacatg gccgtggtgt accacgatcc gactgtcagc
     5281 tccccggccg ccgcccggga ggccgtgtac ctggagtacg gccgccgcct gcaaaccctt
     5341 ctggcctccc tgggcttaga agtacgggac ggcgaggccc ggcgaccttg agcggggagg
     5401 atgccatgga tacctggaag ctgccgtcct tcaagaccga ggaggaattg gaatcccgca
     5461 tgtccgagcc gacggccgcg gtcgtggagg agttccggaa gaatcccgga gacctggcca
     5521 tcctggggat cggcggcaag atcggggtga ccctggggat gatggccgcc cgggcccggg
     5581 acgccgcggg ggtccgatcc cggattctcg gagtctcgcg cttttccgac aaggcggccc
     5641 gggccagact tgaaaaggcc ggagtggagt gcctggagtg cgacctgttg gaccgctcgt
     5701 ccgtggacaa gctccccgac gctccccggg tcgtgttcat ggcgggcaag aagttcggca
     5761 cctcgggctc cgaggagacg acctgggcca tgaacaccgt ggtgcccgcc tacgtggcgg
     5821 ggcggtaccg ggactccgcg atcgtcgcct actccacggg ctgcgtgtac gatgtcgtga
     5881 ccccctactc cgggggctcc tcggaatccg accccccccg ggccgtcggg gactacgccc
     5941 agtccgccct ggggcgggag cgggtcttcc agcacttcag cagggcggag ggaacccggg
     6001 tctgcatact taggctcaac tacgccatcg acccccggta cggggtgctc cacgacatag
     6061 cccggaaggt cgccgcgggg gagggcgtgg agctcgcggt ggagaacttc aactgcatct
     6121 ggcaggggga cgtcctggag cgcaccctcc tggctttccg gcacgccgcg agtcccgcct
     6181 tcgtcctgaa cctcacggga ccggagacgg cttcggtgcg caggaccgcc gaggaattcg
     6241 gccgcctcct gggcaagccc ccggtcttcc ggggcggcgg cgagggcggc cgggcctacc
     6301 tgaacgacgc ggccctctcc ttccggctct tcggctatcc ccgggtaccc ctggcagaga
     6361 tgatccgcat gcaggcggac tggatcgcct ccgggggcgg gtccctgggc aagccgaccc
     6421 atttcgagac caccgacgga acctactaga cggaggcgcg catcgtgaaa agatcggaca
     6481 tcccgacggg actcctggaa accctgcggc gggggaccgt gatccccgcc atccccctgg
     6541 ccctggacgc ggaccgccgt ttcgaccccc ggaggcagaa ggccctggtg cggtactaca
     6601 tcgacgccgg agccgggggc atcgcggcgg gggtccacac gacccagttc gagatccggg
     6661 atccccggca cggactcttc cggccggtgc tctccgcagt ctccgcagcc atagacgagt
     6721 ggtgcggccg ccgcggcacg agggtgctca aggtgggcgg ggtctgcggc cccacccacc
     6781 aggcgtgcca ggaggcggac ttcctggtct ccgcgggata ccacgcgggg cttttgagcc
     6841 tggcgggcca cggggaggac tcgatcgagg acctcctggc ccatgtccgg aaggtggccg
     6901 gacggatccc cgtaatcggc ttctacctcc agcccgccgt ggggggccgg gtcctgccct
     6961 acgagttctg gcggggcctc tgcgagatcg agaacgtcat cgcgatcaag atggccccct
     7021 tcaaccgcta ccgcaccttc gacgtggtgc gcgccgtggc ggaatccggc cgggcgggcg
     7081 aaatcgccct gtacacgggg aacgacgaca acatcgtggt cgacctgctc acggagtacg
     7141 ccgtccggac cgcgggggag acacggcgcc tccggatcgc cgggggcctc ctgggacact
     7201 ggagcgtctg gacccgccgg gccgtcgaac ttttacgctc ggcccgggac ctctcggacg
     7261 cgggtgcccc gataccccaa tccctgctga ccctggccca ggaggtgacg gacgccaacg
     7321 cggcggtctt cgacgcggcc aacggctttg cgggctgcat ccccgggatc cacgaggtgc
     7381 tccggcgcca gggcctcctt tcgggcacct ggtgcctgaa tccccaggag gggttgtcac
     7441 ccgggcaggc ggaggagatc gaccgggtct gccggtccta cccccacctc acggacgacg
     7501 agttcgtgac cgctcacctg ccggagtggc tggcggacta gccggataag aacgatcagg
     7561 actcaccact gagaacgcgt ccggatggct gcgccatccg gacggcgccg aagctcgctt
     7621 cggcgatcta tttgagccgc aatcgacgaa gaccgaaggg cttcgtcgcc gcgagccgga
     7681 cgaacgtccg gctcgcgcgt ctccgtggtg agtccagaaa ttcaaacagc cgctttcttc
     7741 cggaacatct gcgccatcag ggcccggaac cactttgccc ggcccaggac gatgatcagg
     7801 gtcaaaaggc ccaggaccac gcagatgggc cgggtgaaga acggggctac gcttccgtcc
     7861 gagaggatca gggcgcggcg gaggttcttg tccaggatgt cccccaggat gatccccagg
     7921 accagggggg ccatgggata gtccatctcc cgcaggatga agcccagggc gccgaacacg
     7981 atcatgatcc ccacgtcgaa gagccgggcc tggagggcga aggaacccac cacgcagagg
     8041 atgaagatga cgggcatcag cttctggcgc gggacctgga ggaccttgac caggggccgg
     8101 accatggaca ggcccaggac gaacatcatg atggtggcca ggaggaccat ggccacgacc
     8161 tcgtagatga aggtcgggaa ctccagcatg atgaggggtc cgggccgcac cccgtggatc
     8221 agcatggccc ccaggagtac cgccgcgggg gcggagcccg ggatggccag ggtcaggacc
     8281 gggatgatgg ccccgggcac gcaggcgttg ttccccgtct ccgaggccag gagcccctcg
     8341 accgaaccct tgccgaactt ctccctctcc tcgggcttgg cgccccgctt ggccatgtcg
     8401 taggagaccc aggccgcgat gtcctccccc accccgggga tggccccgat gaaggttccg
     8461 atcaggccgg atcgtatgat cgtcttccag tacttgaaga cctcggacag cctggggatg
     8521 acccggtcga tggccgtctt cactacctcg tacttttcca cccgcatcac ggagatgatc
     8581 tccgcgaacc cgaaggcccc gaccatggct ggtatgaggg agatgcctcc ggagaggttg
     8641 gttgagccga aggagaagcg cacgtaggcg tggatcccct ccatgccgat catggccacg
     8701 aagagtccca ggaagcccga gatccagccc ttcagggggt ccttgggagc cgtcaggttc
     8761 ccgcagatga tgatcccgaa gatggagaac cacacgaact cgtaggactg gaagctcagg
     8821 gcgaccgtac cgatcatggg ggtgaagacc agcatggcca gcatgccgat catggacccc
     8881 aggaaggatc ccgtcgtggc gatgccgatg gccttgcccg cctttccctg cagggccagg
     8941 gggtagccgt ccacggtggt ggcggcgttg gcgggggtgc ccggtatgtt gaggaggatg
     9001 gcgctgcggc tgcctccgta gatggccccg atgtacatac agatcaggat caggatggcg
     9061 ttgccgggtg ccatgttgaa cgtgagggtc gtcagcaggg ccacccccat ggtggccgtg
     9121 aggcccggaa gcatgcctat gatgattccc aactgggtag cccagagaga atcgaacagg
     9181 gcccggaagg tgaagaccgt gcccatcgcc tcgccgaaaa gtttcaagcc ttcgaacatc
     9241 ccggactcct tagggaaggt tgacgaggaa gaggtactgg aataccagga agaccgaagc
     9301 cgccgccacg gccgccagga tcgccgcgaa gaggggcttc ttccattgcg cagtcagggg
     9361 aaccttcgtg tcgtactcga aggtgaggat gaacaggaac acgaacagga aggtgagcag
     9421 gggaaaccag atcctgccca gcaggatcac gtagccgatg cagagcacga gggtccgggc
     9481 tatccggacc caggaagacc ggtcgccggt tccggtcccg gcccggtcct ccgcgaaaag
     9541 cctgggcgcg cccttccgca gggatcggat cagcatggaa aggctgagca ggaacagcac
     9601 ggctcccacg aaggccggga ccaccccggg cccggagaag gggctggcgt tgcggtccgc
     9661 catggtcggc atccgcaggg acatgaggag gatcgccgcg gagaacacga tcagaagcat
     9721 cgaagtgacg aggtcggcct tcgtcatggt ccgttcggtc atggttcgct ccagggtcgg
     9781 cgttcggttc cggggcggag ccccttcacg ggaaaagccc gccggcgcgg ggccggcggg
     9841 ctcgtacgaa gcgggggagt caggacaggg gccgcgtcac ggcttgggga tgcccaccgt
     9901 gtccggggag accttggcct tgccggcgtc ccagtagagc caggccacgg gctggtagta
     9961 gctgaaggcc cggtcctggg cttccttgcc gaaggagggt gcgaacatgg cgccccggct
    10021 cttggcgtac tcctggacct tggtggactt catgatcacg ttcttccagg cccgctccac
    10081 ggtgtcgacg acgttcttcg gaacgcctac ggggatgaag atcccgaagt agttcgggcc
    10141 ggtcttgaag tccttgaccc acttggtgat gggcgggatc tccccgaagc cttccaggtt
    10201 caggggcttg tcggacatga ccgccagggg acggatcttc ttgccgcgga tcatgtccgc
    10261 ctgctccacc gccagctggg tggtcatttc ggtctcgccg gacacgcagg cgatgaccgc
    10321 ggggttgccg ccgtcgtagg tgacgtgctt gtactcgatc cccgtgtact tgcggatcag
    10381 ctcgattccg atgtgtcccg cggagctctg gccggcggtg gccacggtga tctggccggg
    10441 cttgctcttg aacgcggcca ggagctcgct gaagtccttg tagggggtca tggcgttgac
    10501 acccaccacg gagacgttcg ccaccgagag gaagacgtgc caatccttgc ggatgtccgt
    10561 gtccagcatg ccctgcacct tgtaggtggc caggtccgcg gcggcgccgg cggtccaggt
    10621 gtagccgtcc ttcggggcgt ccagggcgtt cttggtgccc acggagcccg aggctccggg
    10681 ctggttcacg atcacgatct tcgtacccag ggcggcctcg agttcggccg cgcagacccg
    10741 ggtcacctgg tccgtggacc cgccggctcc ccagggcacg atgatggtga tcggcttgtt
    10801 cggcttccag ttggcctggg ccgacacggc gccgacggcc aggatcagga cgagggcgac
    10861 cgcgagaatt cgagcgctta gtttcatgtc gtgagcctcc ttacggaaac gttcgctttc
    10921 cgaaagtatg gaataccttt ccggcggcgt caagaaaaca atccatggtt cctggaattt
    10981 ttccggcttg acatgggccg agggcgcccc gcatgcttta gccatgacac cctccgcatc
    11041 accccggacg gagcccgaac cggcccgccg ctgccccata gccctcgtga ccgggtccag
    11101 ccgcggcctg gggcggggaa tagccctgga gctcgcggcg gccgggtttt ccgtggccgt
    11161 ccactacgcg ggcaacgagg aggccgcacg ggaaaccctc cgcctgtgcg cggacgccgc
    11221 cgagcggttc cggccggacc gggccccgga gccccgcttc gaggccttcc gggcggacct
    11281 ctccgacccc gccgcccgag ccgccctggc ccgggacgtc ttctcggcct tcgggaccct
    11341 ggacgccctg gtgaacaacg cggggatggc cccccggatc cgggcggaca tcctggatgc
    11401 ctcggaggag tccttcgacg agcttatcgc gacgaacgtc cggggaccct acttcctgac
    11461 ccgggacgtg gcccggctct ggctggccgg accccgggac accccccttc ccggggggcg
    11521 gaaggtcgtc ttcgtcacct ccatctccgc ggtgaccgcc tcggtgaacc ggggggacta
    11581 ctgcatgtcc aaggcggctc tgtccatggc ggccaagctc tgggctgccc gcctggcacc
    11641 ggagggcatc cccgtctacg aggtgcgtcc ggggatcatg ctgaccgaca tgaccagcgg
    11701 agtgaaggag aaatacgacg ccctcctggc ccagggcctg gtgccccagg ggcgctgggg
    11761 cacccccgag gacacgggca aggccgtacg ggcccttttg gaaggggcct tccccttctc
    11821 cgccggaggc atcttcgacg tggacggagg attccacgtc tcgcggctct gagcggccgc
    11881 ggatcggaga ggtaggatga tcgatcttcg caacgcgccg aatcccccgg cactcgccga
    11941 cgcctgcgga cgggcctttt cggccgcgga cgccaagatc cgggccctgg ccgcccgctg
    12001 gaaggcggga tccggagccc cggtgttcac ggacgcgggc cgctacactg cccggggctg
    12061 gaccgagtgg acccagggct tccagttcgg gatgcccctc ctggtccacg aggcttccgg
    12121 ggatccctgg cacctggagt acgggcgttc caagaccctc cgccgcatgg ccccccacct
    12181 gacccacacg ggggtccacg accacgggtt caacaacgtc agcacctacg ggaccctcct
    12241 gcgccacgcc cacgagggcg tattccccgc ggagacctgg gagcggcggt actacgccct
    12301 ggccctcaag gcttccgggg ccgtgcaggc ggcccgctgg acgtccctgc ccgagagcct
    12361 gggcttcatc cggtccttca acggtcccca cagcctcttc gcggacacca tccgctccct
    12421 gcggagcctg gccctgggcc accgcctggg ccacgtcctt ttgggggagc aggacgccaa
    12481 ggcgaacctc ctgggccggc tcctggccca cgcggaaacc accgcccggt acaacgtcta
    12541 cttcgggacg ggacgggacc gctgggacgt gcgcggccgg gtggcccacg agtccgtgtt
    12601 caacaccgtc aacggaagct accgctgcgc ctccagccag cagggctact cccccttcac
    12661 gacctggacc cggggcctgg cctggatcat cctgggctac gcggaggaac tggagttcgc
    12721 ggcgggcctt tcggacgcgg aggtcgcggc ctgcggccta gcctacttcc cggacgcggc
    12781 ttccgtgcgc gcccggttcc tcgagaccgc ccgggcggtc tcggacttct acatcgagaa
    12841 tacacccccg gacgggatcc cctactggga caccgggggg ccccgcctcc gggagatccc
    12901 gggcgccctg gacaggcccg cggacccgga gaatccattc gagcccgtgg actcctccgc
    12961 cgccgccatc gcggcccagg ggctcctgcg cctgggagcc ttcctcacgg ccgccggaga
    13021 ccccgacggg gcccggtaca ccgccgcggg gctccggacg gcggcctcgg tcctctcggc
    13081 cccctatctt tcggagaacc cgaagcacga ggggctgctt ctgcacgcgg tgtaccacta
    13141 ccccaacgcc tgggaccggt ccgcggacgg gagccccgtc ccccacggcg agtcctgcct
    13201 ctggggggac taccacctcc tggagctggc tctgtggatc tcccgcacgg cccggggccg
    13261 gggcggccag cgcttcttcg acatcggcct cgacccggac gggggcggat catgaacgac
    13321 ctctcccgcc tggcgatcca caccgccacc accaagccct gggacctgga aaccgccgtc
    13381 cgcaagtacg cggacgcggg ggtctcgggc atctcggtct ggaaggaagc cctggagggc
    13441 cgggatcccg cggcggccgg ggcggcgatc cgggccgcgg gcctggcgcc cgtgtccctg
    13501 gtgcgggggg gcttcttccc ggccccggac cccgagaccc gggccgtccg gatccgggac
    13561 a
//
LOCUS       DAOTEI010000090        13445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3188_length_13445_cov_5.077670, whole genome shotgun sequence.
ACCESSION   DAOTEI010000090 DAOTEI010000000
VERSION     DAOTEI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_13445_cov_5.077670"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1334)
                     /locus_tag="P5313_09720"
     CDS             complement(57..1334)
                     /locus_tag="P5313_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY80683.1"
                     /translation="MKRKTILIVDESDLFRDYVKSRLSQFNLEVATAINGLDGVTKIR
                     NLLPDAVLLDYHLTRKSCKEVLEEKARDQNASGIPVILSAQKIDKNRLLELMSFNIRK
                     VLIKPIKIDTLYQTLSELLGIRFEIDTTPCVIEAHVNDAILFIEIAQGMNREKIELLR
                     FKIAELLELYGIARPRVLIMISDMELSFVDGPNLEALLDTVLSASRAKNRHLKVLTRS
                     SFVRAFVQGRSAYADIEVVDDLRKAMDGLLADGGGGDPGATRGEIISERILSADRKES
                     SQESVEMRFEAEARKPLSLETLRETGKGLSVAAVDDDFVIQELIKTSFGAISAQVTAY
                     SEGRSFLEAMRAGASYDLVFLDILMPGMNGYEVLDGLREGGIDVPVIILSALGQKEAV
                     LKAFQRGVKSYLIKPLKPEQILRKSLEILKANF"
     gene            1497..3008
                     /locus_tag="P5313_09725"
     CDS             1497..3008
                     /locus_tag="P5313_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY80684.1"
                     /translation="MIRVLYAAGDEAERAAVAAIRDADLEISFPAGVPEIEARIAAGT
                     ADVIVTDFSFAAGGFADWLSLWPLPAVLLVNPGADPARAESAVRDESSVFLVRDPAGE
                     WLRYLPAQIRKIRNMRESITRHNAFLSITERQYLNLLQAIPDIVYMLDGNGNFLYLNE
                     SIRSLGWEPSKLIGQHFSRIVHPDDVPNISKEFVLDRYTGTVTGPEQAPKLFDERRSG
                     SRMTRNLEVRLVHGFQSADPFLGSVNAYGEVSSTGMELPEYENLRVGTVGIIRDITHR
                     KEHQRKLESDLEAKEILLKEIHHRVKNNLQVVSSLLNLQENSVNDDSVRGVFLECQTQ
                     IQSMAMVHEQLYRSMDLEGVEMEPYLERLAEYLSGIYEAAYQGVEYRVQAPGVRVDLD
                     TAIPLGIITNELVSNSFKHAFPGGRTGKVFIGIHDRGTSWELEVSDDGVGFSGSKERP
                     ACGSEHDRRCYPSFSSGLGTELVTALASQIKGSERKVDDGGARTYILFPKKPV"
     gene            complement(3075..4502)
                     /locus_tag="P5313_09730"
     CDS             complement(3075..4502)
                     /locus_tag="P5313_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80685.1"
                     /translation="MNRASGMIAGALALALWAAAPLPAQPADPARMRPRPELRDIGAL
                     REPLAAEVFLEAALALSGGTPEEAARARAALAAALESAEGSLPGPSDPGARAEALLSF
                     LHARILKRYVESQTRVDRAVLTGEYNCVSSAVLYAYLAKKAGLSVSGVRTPDHALCVV
                     ELPGRPVDVETTNRYGFDPGSRKEFLDSFGRTTGYAYVPPGRKDARRGMGDREFLGLI
                     LSNRIAELELRGRWEESVGLAFDYDALVGSPESRTFLLDRISNLTAGRHARNDWDGAF
                     SALAAAAAALGPSPRLAELEAVTAQAALSWFGSRSDWSGGLDYARRLPPSAVPDPRVQ
                     GLVRALAEGRLRDSVRTAPFAEAVPAVEDARAEGILDARGRAELLEYLYVNEANAEAK
                     RSGWLAGIAVLERGLDRVPGSGALTSARRVFRSNWTAEVHNRFAAHFNARRFEEAARV
                     LEEGLALDPGNPTLLKDFETLRAAR"
     gene            4656..5591
                     /gene="trxB"
                     /locus_tag="P5313_09735"
     CDS             4656..5591
                     /gene="trxB"
                     /locus_tag="P5313_09735"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRY80686.1"
                     /translation="MEPQWDLIVIGAGGAGLGAAQYGARANLKTLLLEEMAPGGQALV
                     IDRLENYPGIAEPVSGFDFADLMRRQAEQFGAEIRSSGVSALRREGNLFVLDTSDGPV
                     STWAVILATGAKHRHLEVPGETEFSGRGVSYCATCDGPFFKGKRILVVGGGDAACDEA
                     MFLSKLTDRVILVHRKDRFRAQKALAARVLANPRIETRFHHAVKEIHGSKQVEAVTLV
                     RTDTGEEYREPVDAVFVFVGSLPQTSLAGEAEKDETGSLITNDRMETSVPGLFAAGDV
                     RATPFRQLITAVADGAVAAHSASQYIDEIRGEAYR"
     gene            5603..7351
                     /locus_tag="P5313_09740"
     CDS             5603..7351
                     /locus_tag="P5313_09740"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HRY80687.1"
                     /translation="MRTSARTLLLISSVAVLGAAAFLSSLSAQSMSGHRTSLPRDEKP
                     RETPDRGRDFWASGEDAASAEALLDGLSDEEVLGQIFMMAYPGTNPPALLYRWIERRA
                     LGGIKVFGWNAEDTEVLAGAVRGIQEAASRTDHRIPPFIATDQEGGWIRHVKGATSVT
                     PGNMAIGASAIPRDAYRSALYIGKELAALGITMNFAPTVDLALRPRSVIIGPRAFSSD
                     PSVAAVLAAAYVRGMEDAGVLPTAKHFPGHGDTELDSHGILPVIPVDERTLWERDLLP
                     YRMLVAEGLPGIMSGHLAFPRITGDRTPASLSRYFITGILRDRMGFDGLVVTDDLFMT
                     AAAVPGGFPEICVRAVMAGNDILMISRILEFSEEAWMRLLREYRTDPEFKARVRQSAL
                     RILRAKQAYLKPKGPAGVAPRSERASLLQSRDARIFFTDQAYRSATVLEGAGLPLAPG
                     AGRVLLAGSFSDFFAEGTARYPGADSFRFTYQPGPEVLDAELESFRSRARGAGAVVVG
                     VANSTGAAFARAASRMGVPVYLVSVLSPVFALDAGPIAGGVAVYNGSRESYAAAFAVL
                     SGAVEGCGILPIPRNP"
     gene            7348..8214
                     /locus_tag="P5313_09745"
     CDS             7348..8214
                     /locus_tag="P5313_09745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY80688.1"
                     /translation="MIPGSDGAGWRRLGPEDAPALREFLVPREDYAAGFTGRLLRSGG
                     LRIPGPLEGGVFGCFAPEGLRDAVLWTRGGTVFPAFKDASDIPEGERLGRFFKGGAIA
                     SFLGAGPDVEALEGILGLEPRVATPYRSLYLDPIPPDPSRPPPDPRAAVRTAGPRDAA
                     ALFPLHSAYEREEVVTEIHRFDARASRAALEGILASEIVAVAELDGRAAATARTNARG
                     FRTWQIGGVYVIPELRGRGWGRYVVSHLVGLISRAGKAAGLFVKEGNPAARSLYLSMG
                     FLDAGPFRVDYL"
     gene            complement(8211..8978)
                     /locus_tag="P5313_09750"
     CDS             complement(8211..8978)
                     /locus_tag="P5313_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRY80689.1"
                     /translation="MADMKVQSLDRTFGILQMLAKDPNGLTLAAIAEGEDLPKSTAFR
                     LLAVLARHEYVRKNPDTNLYRLGPAFLELSSRYLNSLELKTESAPYMSELAAALGTIV
                     FLARRQGNRMVYIDKQDQYTSLRKYSIIGQQKPLYCTSLGKALILDMPDDEIRALLAG
                     ESFEKFGPNTHTGVEDLLADLHACRGRGWTRDNEEAEPGVNCVAAPIRDYRGEVISSV
                     STAWYLDSRPDLSPDKVATQVLRAAEGISHSMGWTKD"
     gene            9129..9959
                     /gene="kduI"
                     /locus_tag="P5313_09755"
     CDS             9129..9959
                     /gene="kduI"
                     /locus_tag="P5313_09755"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006533834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HRY80690.1"
                     /translation="MDIRENVNSEYAKTLDTEGLRSRFLVSGLFKPGEISLTYSHVDR
                     MIAGGVTPVSGPLALAATKDLGTEYFLERREMGVINIGGPGAVTVDGTRYEMKSRDGL
                     YVSMGSKEVSFESADPARPAKFYLNSAPAHRTCPTRLVTMAQAKRVEMGSAAECNQRV
                     INQFIHPAVLESCQLVMGMTTFSEGSVWNTMPVHTHERRMEVYLYFDMPADRVVFHFL
                     GKPEETRHLVVRNEEAVISPSWSIHSGVGTGPYTFIWGMAGENQTFTDMDAVPMSVLR
                     "
     gene            9978..10739
                     /gene="kduD"
                     /locus_tag="P5313_09760"
     CDS             9978..10739
                     /gene="kduD"
                     /locus_tag="P5313_09760"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013318066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HRY80691.1"
                     /translation="MILDAFKLDGKVAVVTGSSTGLGQGIAVALAEAGADVAGVDYVA
                     SPETKRTVEALGRRFLEIVADLSSIAPAVGIVEDTVRTLGRLDILVNNAGIIRRQDAV
                     DFTEKDWDDVMNINAKTVFFLSQAAARRYIAQGSGGKIVNIASMLSFQGGIRVPSYTA
                     SKSAVMGVTRLMANEWAKHGINANAIAPGYMATNNTAALRADEERSKAILDRIPAGRW
                     GTPADVQGAAVFLASRASDYVNGYTLAVDGGWLAR"
     gene            10748..11206
                     /locus_tag="P5313_09765"
     CDS             10748..11206
                     /locus_tag="P5313_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HRY80692.1"
                     /translation="MIATPLENLGRYKTLSPSLAKAIDWLEAGGWESLPEGRHEIAGT
                     EVYALVSRYTSKAPQEARYETHRDYIDIQLLVSGRETVEARSAVGLSVSVPYKPDIEL
                     YDFPEPGTCHEFLLAPGTALVFFPEDAHRPGLARDGVPEPIHKVVVKAAR"
     gene            complement(11203..12852)
                     /locus_tag="P5313_09770"
     CDS             complement(11203..12852)
                     /locus_tag="P5313_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY80693.1"
                     /translation="MRVTDPAPGRHGRRSASAAAILLILAGAAGCSRPPRNSAAFGGS
                     SPESRPSRVVVVTDEFYPPYVMRNEVGELEGIVVDLWEIWERITGIPVEIRAYSWSEA
                     QDRFARGEGDVIETIFDTPERRERLSFSPPYARIPVPVFTHASITGIAGPEDLRGFRI
                     AVKSGDAAVAELIRRGVSDLSLYRSYKDIVEAAARHEVRVFCVDGPPAYHYLYRLGID
                     REFRQAFILNEGAFSRAVRRGDESLLATVQRGFDALPPGQAAAVNRRWMGSELPRVPD
                     WRILGGLGAAAALLVAFLAGATVSLRRRVERATRELKEKISQLEASETRITRALREKE
                     VLLQEIHHRVKNNLQIISSLLRLQAEDLASPAARDLLLGTQSRIAAMADLHDRLYNSR
                     DLERIDAGEYLRSIGDTLATTFRFDGFRFDTQALTVSIETALPLGLLVNECAINAVKY
                     AYPDSRTGSVEIGLRRDGPDCVCSVRDFGRGLPAGVVPETAGTLGFTIVRSLADQLRG
                     RVRFDSSAGFLAELRFPLPEEQVAAAAPGASPDGSPSGGGF"
     gene            12961..>13445
                     /locus_tag="P5313_09775"
     CDS             12961..>13445
                     /locus_tag="P5313_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRY80694.1"
                     /translation="MKLRARILLFSLIPVILTLGTVAGITAVRTLEQSRKDAVDLLEA
                     LSKEHAAEINVELEVAMDAARTLAQIFEGYESLHADERREDYSKMLRSIVEKNPGFLG
                     ASTGWEPNALDGRDREFVDAPGHDGTGRFIPYWSRFEGPVELSPLVDYDKEGAGDYYI
                     LP"
BASE COUNT         2174 a         4529 c         4564 g         2178 t
ORIGIN      
        1 cgaatgtctc ccggaacggg gcacccggac ggggttcgcg catcggcgtc tccctgttag
       61 aagttggctt tgaggatctc cagggacttg cgcaggatct gctcgggctt caggggtttg
      121 atcaggtagc tcttgactcc ccgctggaag gccttgagca cggcctcctt ctgccccagg
      181 gccgagagga tgatgaccgg cacgtcgatc cccccctccc ggaggccgtc caggacctcg
      241 tacccgttca tcccgggcat caggatgtcc aggaagacga ggtcgtagct tgctccggcc
      301 cgcatggcct ccaggaagga gcgcccctcg gagtacgcgg tgacttgggc ggatatggca
      361 ccgaagctgg tcttgatgag ctcctggatg acgaagtcgt cgtccaccgc cgccacggac
      421 agccccttgc ccgtctcccg cagggtttcc agggacaggg gtttccgggc ttccgcctcg
      481 aaccgcatct ccacggattc ctggctcgat tccttgcggt ccgccgacag gatcctttcg
      541 gatatgatct ctccccgggt cgcccccgga tccccgcctc ccccgtccgc cagcagcccg
      601 tccatggcct tcctgaggtc gtccacgacc tcgatgtccg cgtacgcgct ccggccctgc
      661 acgaaggcgc ggacgaagga ggaccgggtc aggaccttga gatggcggtt cttggcccgg
      721 gaggccgaaa ggaccgtgtc caggagggcc tccaggttcg ggccgtccac gaaggacagc
      781 tccatgtccg agatcatgat caggacccgg ggacgggcga tcccgtacag ctccaggagc
      841 tccgcgatct tgaagcgcaa aagctcgatc ttctcccggt tcatcccctg ggcgatctcg
      901 atgaagagga tggcgtcgtt cacgtgcgcc tcgatcacgc agggggtcgt gtctatctcg
      961 aaccggatcc ccaggagttc ggacagggtc tggtagaggg tgtcgatctt gatcggcttg
     1021 atcaggactt tccgaatatt gaaggacatg agctccagca ggcggttctt gtcgatcttc
     1081 tgggcgctca ggatgacggg aatgccggag gcgttctggt cccgggcctt ttcctccaga
     1141 acctccttgc aggacttccg ggttaggtgg taatccagga ggacggcgtc gggcaggagg
     1201 ttccggatct tcgtgacccc gtccagcccg ttgatggccg tcgcgacctc caggttgaac
     1261 tgggatagac ggcttttcac gtaatcccgg aagaggtccg actcgtccac gatcaagatc
     1321 gtctttcgct tcattcttta ctatggttcg gcgcgtttcg ggcttacctt gacaggtccc
     1381 tccgggtgcc tatagtttta ccccatgcgg gaaatcggat cgaggcgggg aacccggaca
     1441 ttcgggcgcg gactcgggct tccgggtgcc gatgccggaa cggtgccgac ttgggcgtga
     1501 tccgggtcct gtacgccgcg ggggacgagg cggagcgcgc cgccgtcgcc gccatccggg
     1561 atgccgacct ggagatctcc tttcccgccg gagtgcccga gatcgaggcc cgcatagccg
     1621 cggggaccgc ggacgtcatc gtgacggatt tctcgttcgc ggccggaggt ttcgcggact
     1681 ggctctccct ctggcccctg cccgccgtcc tcctggtgaa tcccggggcg gatccggccc
     1741 gggcggagag cgccgtccgg gacgagtcca gcgtgttcct ggtccgggat cccgcgggcg
     1801 agtggcttcg ctaccttccg gcccagatcc ggaagatccg gaacatgcgg gagagcatca
     1861 cccggcacaa cgccttcctc tccatcaccg agcgccagta cctgaacctg ctccaggcta
     1921 tcccggacat cgtgtacatg ctggacggga acgggaattt cctctacctg aacgaatcga
     1981 tccggagcct gggctgggag ccttccaagc tcatcggcca gcatttcagc cggatcgtgc
     2041 accccgacga cgttccgaac atcagcaagg aattcgtcct ggaccggtac acggggaccg
     2101 tgacgggccc ggagcaagcc cccaagctct tcgacgaacg gaggtcggga tcccggatga
     2161 cccgcaacct ggaggtgcgc ctcgtccacg gtttccaatc ggcggatccc ttcctgggtt
     2221 ccgtgaacgc ctacggggag gtctccagca ccggcatgga gctcccggag tacgagaacc
     2281 tccgggtcgg caccgtgggc atcatccggg acatcacgca ccgcaaggag caccagcgca
     2341 agctggagag cgacctggag gccaaggaga tcctgctcaa ggagatccac caccgggtca
     2401 agaacaatct ccaggtggtg tccagcctcc tcaacctcca ggagaattcc gtgaacgacg
     2461 attccgtccg gggggttttc ctggagtgcc agacccagat ccagtccatg gccatggtcc
     2521 acgagcagct ctaccggtcc atggacctgg agggggtcga gatggaaccc tacctcgagc
     2581 ggctggcgga atacctgtcc gggatctacg aggccgcgta ccagggggtg gagtaccggg
     2641 tccaggcccc gggcgtccgg gtcgacctgg acacggccat acccctgggc atcatcacca
     2701 acgaactggt ctccaattcc ttcaagcacg ccttccccgg gggaaggacc ggaaaggtct
     2761 tcattggcat ccacgaccgg ggcacgtcct gggagctcga ggtgtccgac gacggggtgg
     2821 gattctccgg ctccaaggaa cggccggcct gcggatccga gcacgaccgg cgctgctatc
     2881 cctcgttctc cagcgggctg ggcacggaac tcgtaaccgc cctggcgtcc cagatcaagg
     2941 ggagcgaacg gaaggtcgac gacggcgggg cccgcaccta catcctcttc cccaagaaac
     3001 cggtctagca ggtcgtcgag tccctcgagg cagtacaact ccctgccggt gtcccgtccc
     3061 ggggccgcgc ggactcatcg agccgcccgc agggtctcga agtccttgag cagggtcggg
     3121 ttgccgggat ccagggccag accctcctcc agaacccggg cggcctcctc gaatcgccgg
     3181 gcgttgaagt gcgccgcgaa ccggttgtgg acctccgccg tccagttgga tcggaacacg
     3241 cggcgggcgg atgtcagggc cccggagccc ggcacccggt ccagtccccg ctccagcacg
     3301 gctatgccgg ccagccagcc ggatcgcttc gcctcggcgt tggcttcgtt gacgtacagg
     3361 tattcgagga gctccgcccg gcctcgcgcg tccaggatcc cctccgcccg ggcatcctcc
     3421 accgcgggca ccgcctcggc gaagggcgcc gtccggaccg agtcccgcag gcggccctcc
     3481 gccagggccc ggaccagccc ctggacccgc ggatccggga cggcggaggg cggaagcctc
     3541 cgggcgtagt ccaggccgcc gctccagtca ctccggcttc cgaaccacga gagggcggcc
     3601 tgggccgtga cggcttcgag ctcggccagc cggggggacg gacccagggc agcggccgcg
     3661 gccgcgaggg cggagaaggc tccgtcccag tcgttccggg cgtggcggcc ggccgtgagg
     3721 tttgagattc tgtccagcag gaaggtccgg gattccgggg acccgaccag ggcgtcgtag
     3781 tcgaaggcca ggccgaccga ttcctcccac ctgccccgga gctccagttc cgctatccgg
     3841 ttggacagga taaggccgag gaattcccgg tcccccatgc cgcggcgggc atccttgcgg
     3901 cccgggggga cgtaggcgta gccagtggtc cgcccgaagg aatccaggaa ctccttgcgg
     3961 gaaccgggat cgaacccgta ccggttcgtg gtctccacgt ccaccggccg tcccgggagc
     4021 tcgaccacgc agagggcatg gtccggggtg cggaccccgg acacgctcaa gcccgccttc
     4081 ttggccaggt aggcgtagag caccgcggag gagacgcagt tgtactcgcc cgtgagcacg
     4141 gcacggtcga cccgggtctg gctctccacg tagcgcttca ggatccgggc gtgaaggaag
     4201 gaaaggaggg cttcggcccg ggctccggga tccgaggggc ccggcaggga gccctccgcc
     4261 gattccaggg ccgcggccag ggcggcccgg gcccgggccg cctcctccgg ggtgccgccg
     4321 gagagcgcca gggcggcctc caggaagacc tccgcggcca ggggttcccg cagggcgccg
     4381 atgtccctca gttccggccg ggggcgcatg cgcgccgggt ccgccggctg ggcgggcagg
     4441 ggcgccgccg cccagagggc cagggccagg gccccggcga tcattccgct tgcccgattc
     4501 atatcctgtc ctgtatagtc aaagctgcgg gaagcggaat ggttactgat cttttaagct
     4561 tagtccgtcc gaccgggtac agcggatccc attctggatc ttttcgtgaa aaacatatat
     4621 attagaaaat gtatattatc gcctcggagg agcctatgga accccagtgg gatctgatcg
     4681 taatcggagc gggcggcgcg ggcttgggcg ccgcccagta cggagcccgg gccaacctga
     4741 aaaccctgct cctcgaggag atggctccgg gcgggcaagc cctggtgatc gaccgcctgg
     4801 aaaactaccc cgggatcgcc gaacccgtga gcggattcga cttcgcggac ctcatgcgcc
     4861 ggcaggcgga gcagttcggg gccgagatcc ggtcctccgg ggtcagcgcc ctccgcaggg
     4921 aggggaatct tttcgtcctg gacacctcgg acggacccgt atcgacctgg gccgtgatcc
     4981 tggccaccgg ggccaagcat cggcacctgg aggttccggg tgagaccgag ttttccggcc
     5041 ggggcgtgtc ctactgcgcg acctgcgacg gtcccttctt caagggcaag cggatcctcg
     5101 tcgtgggagg cggggacgcg gcctgcgacg aggcgatgtt cctctccaag cttacggacc
     5161 gggtgatcct ggtccaccgg aaggatcgtt tccgggccca gaaggccctg gcggcgcggg
     5221 tcctggcgaa cccccggatc gagacgaggt tccaccacgc ggtcaaggag atccacggct
     5281 ccaaacaggt ggaggccgtg accctggtgc ggacggatac cggagaggaa taccgggaac
     5341 ccgtggacgc ggtcttcgtc ttcgtcggtt ccctgcccca gacctccctc gcgggggagg
     5401 ccgagaagga cgagacgggc tccctgatca cgaacgaccg catggagacc tccgtcccgg
     5461 gcctcttcgc cgcgggcgac gtccgggcca cccccttccg ccagctcatc accgccgtgg
     5521 cggacggtgc ggtggccgcc cacagcgcct cccagtacat cgacgaaatc cggggcgagg
     5581 cctaccggta ggccggacgg gtatgcggac gtccgctcgg accctgctcc tgatctcttc
     5641 ggtcgccgtc ctcggcgccg cggccttcct gtccagcctg tccgcccagt ccatgtccgg
     5701 ccaccggacc tccctgcccc gggatgagaa accgcgggag accccggacc ggggccggga
     5761 tttctgggcc tccggagagg atgccgcctc tgcggaagcc ctgctggacg ggctgtcgga
     5821 cgaggaggtt ttaggccaga tctttatgat ggcctacccg ggcacgaatc cccccgccct
     5881 gctgtaccgc tggatcgaac gccgcgccct ggggggcatc aaggtcttcg ggtggaacgc
     5941 cgaggacacg gaagtcctgg ccggcgccgt ccgggggatc caggaggccg cctcccgcac
     6001 ggatcatcgc atccccccct tcatcgcgac ggaccaggag ggcggctgga tccggcacgt
     6061 gaagggcgcc accagcgtca ccccgggcaa catggccatc ggggcctccg ccatcccccg
     6121 ggacgcgtac cggtccgcgc tctacatcgg caaggaactg gccgccctgg ggatcacgat
     6181 gaacttcgcg cccaccgtgg acctggccct gaggccccgc agcgtcatca tcggacctag
     6241 agccttctcc tcggacccct ccgtcgcggc ggtgctcgcg gccgcctacg tgcggggcat
     6301 ggaggacgcc ggggtcctgc ccacggccaa gcactttccc ggccacgggg acacggaact
     6361 ggattcccac gggatcctcc cggtcatccc cgtggacgag aggaccctct gggaacggga
     6421 tctcctcccg taccgcatgc tcgtggcgga gggcctgccc gggatcatga gcggccacct
     6481 ggccttcccg cggatcaccg gagaccgcac ccccgcctcc ctctcccggt acttcatcac
     6541 cgggatactc cgggaccgga tgggcttcga cggactggtg gtgaccgacg acctgttcat
     6601 gaccgcggcc gccgtgcccg gcggattccc ggagatctgc gtccgggccg tgatggccgg
     6661 gaatgacatc ctcatgatct cccggatcct ggagttctcc gaggaagctt ggatgcgcct
     6721 gcttcgggag tatcggaccg atccggagtt caaagcccgg gtccggcagt ccgccctccg
     6781 catcctgcgg gccaagcagg cctacctcaa accgaagggc cccgccgggg tcgcgccccg
     6841 gtcggaacgc gcgtcgctcc tgcagagccg ggacgcccgg atcttcttca cggatcaggc
     6901 ctaccgcagc gccaccgtcc tggagggagc cgggctcccc ctggcccccg gcgcgggacg
     6961 ggtcctgctg gccggcagtt tttccgactt cttcgccgag gggaccgcga ggtaccccgg
     7021 ggcggactcc ttccggttca cctaccagcc cggtccggag gtgctggacg ccgaactgga
     7081 atccttccgg agccgggccc ggggagccgg ggccgtcgtg gtgggcgtgg cgaactccac
     7141 gggggcggcc ttcgcgcgcg ccgcctcccg gatgggggtc ccggtctacc tggtctccgt
     7201 cctgtccccc gtgttcgccc tggacgcggg tcccatcgcc gggggggtgg cggtctacaa
     7261 cggttcccgg gagtcctacg cggcggcgtt tgcggtcctg tccggcgccg tggaaggctg
     7321 cgggatcctg cccatacccc ggaatccatg atccccggat ccgacggggc cggatggcga
     7381 cgcctcgggc ccgaggacgc cccggcgctc cgggagttcc tggtcccccg ggaggactac
     7441 gcggcgggct tcacgggccg gctcctccga tccggggggc tgcggatccc gggcccgctg
     7501 gaggggggcg tgttcgggtg cttcgccccc gaaggcctcc gggacgcggt gctctggacc
     7561 cggggcggga ccgtgttccc cgcgttcaag gatgcctcgg acattccgga aggcgagcgt
     7621 ctggggcggt tcttcaaggg aggggcgatc gcgtccttcc tgggagccgg gccggatgtc
     7681 gaagccctgg agggcatcct ggggctcgag ccccgggtcg ccacacccta tcgctccctg
     7741 tacctggacc cgatcccccc ggatccctcc cgcccgccgc cggacccgcg agcggccgtg
     7801 cgaaccgccg gaccccggga cgcggcggcc ctcttccccc tgcattccgc ctacgagcgg
     7861 gaggaggtcg tcacggagat ccaccgcttc gacgcccggg cttcccgggc cgccctggag
     7921 ggaatcctgg cctccgagat cgtggcggtg gcggaactgg acggccgggc ggcggccacg
     7981 gcccgtacca acgcccgggg attccggacc tggcagatag gcggggtcta tgtgatcccc
     8041 gaactgcggg gccggggttg ggggcggtac gtggtgtccc acttggtcgg attgatttcc
     8101 cgggcgggca aggccgcggg actgttcgtg aaggagggca acccggccgc ccggagcctg
     8161 tacctttcca tgggtttcct ggacgcgggg cccttccggg tggactacct ctagtccttg
     8221 gtccatccca tgctgtggga gatcccctcc gccgcccgca ggacctgggt ggcgaccttg
     8281 tcggggctca agtcgggccg gctgtccagg taccaagccg tgctcaccga ggagatgacc
     8341 tcgccgcggt aatcccggat gggggcggcc acgcagttga cccccggttc cgcctcctcg
     8401 ttgtcccggg tccacccgcg ccccctgcag gcgtgaagat ccgcgaggag gtcctccacc
     8461 cccgtgtggg tgttgggacc gaatttctcg aaggattccc ccgcaaggag ggcacggatc
     8521 tcgtcgtccg gcatgtccag gatcagggcc ttccccaggg aggtacagta cagaggcttc
     8581 tgctggccga tgatggagta tttccggagg ctcgtatact ggtcctgctt gtcgatgtag
     8641 accatgcggt tcccctgcct ccgggccagg aagacgatgg tccccagggc ggcggccagc
     8701 tcgctcatgt agggggcgga ttcggtcttg agctctaggc tgttcaggta gcggctggac
     8761 agctccagga aagccgggcc caggcgatac aggttggtat cggggttttt ccgcacgtac
     8821 tcgtgccggg ccaggaccgc cagcagccgg aacgcggtgc tcttgggaag atcctcgccc
     8881 tccgcgatcg cggccagggt caggccgttg ggatccttgg ccagcatctg gaggatgccg
     8941 aaggtgcggt cgagggattg gactttcata tccgccatgg gacctcccgg ggaatcgata
     9001 cggaccatcg ccagtttagt cttgactttc taaatatgca agttacaata tggaatatag
     9061 ttccacaata cgaaacgtac gaatataaga tatggtcgag agggtttctc gacgccaagg
     9121 agattgaaat ggatatccgt gaaaacgtca attccgagta cgccaagacc ctggacaccg
     9181 agggcctgcg ttcccggttc ctggtctccg ggctcttcaa acccggggag atttccctga
     9241 cctacagcca cgtggaccgg atgatcgccg ggggcgtcac ccccgtgtcc gggcccctgg
     9301 ccctggccgc caccaaggac ctgggcacgg agtacttcct ggaacgtcgg gagatggggg
     9361 tgatcaacat cgggggaccc ggagccgtga cggtcgacgg gacccggtac gagatgaaaa
     9421 gccgggacgg cctctacgtc tccatgggct ccaaggaagt gtccttcgag agcgccgatc
     9481 cggcccggcc cgccaagttc tacctgaaca gcgccccggc acatcggacc tgcccgaccc
     9541 ggctcgtgac catggcccag gccaagcggg tcgagatggg gagcgccgcc gaatgcaacc
     9601 agcgggtgat caaccagttc atccatcccg cggttctgga gtcctgccag ctggtcatgg
     9661 gcatgaccac cttctccgaa ggcagcgtct ggaacaccat gcccgtgcac acccacgagc
     9721 gccgcatgga ggtgtacctg tatttcgaca tgcccgcgga ccgcgtggtc ttccacttcc
     9781 tgggaaaacc ggaggagacg cgccacctgg tggtgcgcaa cgaggaggcc gtcatcagcc
     9841 cttcctggtc catccactcc ggggtgggca cggggccgta caccttcatc tggggcatgg
     9901 cgggggaaaa ccagaccttc acggacatgg acgcggtgcc catgtcggtc ctaagataac
     9961 ggaaggagag gacgaacatg atcctggacg ctttcaagct ggacggcaag gtggccgtcg
    10021 tgacgggcag ttccaccggg ctggggcagg gcatcgccgt ggccctggcc gaggcggggg
    10081 cggacgtagc gggggtggac tacgtggcct ccccggagac gaaacggacg gtcgaggccc
    10141 tgggccgtcg tttcctggag atcgtggcgg acctctcgtc catcgcgccg gcggtcggta
    10201 tcgtcgagga caccgtgcgg accctgggcc gcctggacat cctggtcaac aacgcgggga
    10261 tcatccgcag gcaggacgcc gtcgatttca ccgaaaaaga ctgggacgac gtcatgaaca
    10321 tcaacgccaa gacggtcttc ttcctgagcc aggcggcggc ccggcgctac atcgcccagg
    10381 gctcgggggg caagatcgtc aacatcgcct ccatgctttc gttccaaggc ggcatccggg
    10441 ttccctccta cacggcctcg aagagcgccg tcatgggggt gacccggctc atggccaacg
    10501 agtgggccaa gcacgggatc aacgccaacg ccatcgcccc gggctacatg gccaccaaca
    10561 acaccgccgc cctccgggcg gacgaggagc ggagcaaggc catcctggac cgcatccccg
    10621 cggggcgctg gggcaccccg gcggacgtgc agggggcggc ggtcttcctg gcctcccggg
    10681 cctcggacta cgtgaacggc tacaccctgg ccgtggacgg cggctggctg gcccggtagg
    10741 aggagacatg atcgcgacac ccctggagaa cctgggcagg tacaagaccc tgagcccgag
    10801 cctggccaag gccatcgact ggctggaggc gggcggatgg gagtccctcc cggaaggccg
    10861 gcacgagatc gcgggaaccg aggtctacgc cctggtttcg cgctatacct ccaaggcccc
    10921 gcaggaagca cgctacgaga cccataggga ctacatcgac atacagctgc tcgtctccgg
    10981 ccgggagacc gtggaggccc ggagcgccgt gggcctttcg gtctcggtgc cctacaagcc
    11041 ggacatcgag ctctacgact tcccggagcc ggggacctgc cacgagttcc tcctggcccc
    11101 gggtacggcc ctggtcttct tccctgagga cgcccaccgc cccggcctgg cccgggacgg
    11161 ggtcccggag ccgatccaca aggtggtcgt gaaggccgcc cgctagaagc ctcccccgga
    11221 aggtgacccg tccgggcttg ccccgggggc ggcggccgca acctgctctt cgggcagggg
    11281 aaagcggagc tccgccagga agccggcgga ggagtcgaac cggacacgcc ctcggagctg
    11341 gtccgccagg ctgcggacga tggtgaagcc cagggttccg gcggtctcgg ggaccactcc
    11401 cgccgggagc ccccgaccga aatcccggac ggagcagacg cagtccgggc cgtcccggcg
    11461 aagccctatc tcaacggacc cggtccggct gtccgggtag gcgtacttaa ccgcattgat
    11521 cgcgcattcg ttgaccagga ggcccagggg cagggccgtc tcgatgctga ccgtcaaggc
    11581 ctgggtgtcg aagcggaacc cgtcgaaccg gaaggtcgtg gccagggtgt cccctatgct
    11641 tctcagatac tcccccgcgt cgatccgctc caggtcccgg ctgttgtaaa gccggtcgtg
    11701 gaggtccgcc atggcagcga tacggctctg ggttcccagc aggaggtccc gggccgccgg
    11761 ggacgcgagg tcctccgcct ggagccgcaa aaggctggag atgatctgca ggttgttctt
    11821 gacccgatgg tggatctcct ggagcaggac ttccttttcc cggagcgccc gggttatccg
    11881 ggtctcgcta gcctccagct gggagatctt ctccttgagc tcccgggtag cccgctccac
    11941 cctgcggcgc aggctgaccg tggcgcccgc caggaaggcc accaggaggg cggcggcggc
    12001 cccgaggcct cccaggatcc gccaatccgg aacccggggg agctcgctcc ccatccaccg
    12061 gcggttgact gccgcggcct gcccgggagg aagggcgtcg aatccccgct ggaccgtcgc
    12121 gagcagggac tcgtctcccc ggcggaccgc ccggctgaag gctccttcgt tcaggatgaa
    12181 agcctggcgg aattcccggt cgatcccgag cctgtacagg tagtggtacg cgggggggcc
    12241 gtcgacgcag aacacccgga cttcatgccg cgcggccgct tccacgatat ccttgtagct
    12301 ccggtagagg gaaaggtcgg agaccccccg tcggatcagc tcggccacgg cggcgtcgcc
    12361 ggacttcacc gcgatccgga atcccctcag gtcctctggg ccggcgatcc cggtgatgga
    12421 ggcatgggtg aagaccggaa ccgggatccg ggcgtaggga ggggagaagg acagccgctc
    12481 ccggcgttcg ggggtgtcga agatcgtctc gatcacgtcc ccctcgcccc gggcgaaccg
    12541 atcctgggcc tccgaccaag aataggcccg gatctccacg ggaatcccgg tgatccgctc
    12601 ccagatctcc cacaggtcca ccacgatgcc ttccagctcg ccgacttcgt tccgcatgac
    12661 gtagggggga tagaactcgt cggtgaccac gacgacgcgg gagggccggg attccggaga
    12721 agagcctcca aaggcggcgc tgttccgggg cggccgggag catccggcgg ccccggcgag
    12781 gatcagcagg atcgccgccg cggaagcgga ccggcgtccg tgccgcccgg gggccggatc
    12841 ggtgacgcgc atgggaaaag attatagccg ggatcgggaa tcgcgcaagg cgcgaggggc
    12901 ggcacctcgg gtttgcggcg gacgcgggca tgcactatcc ttacctcacc ggaggagacc
    12961 atgaaactgc gcgcccggat actcctgttt tccttgatcc ccgtcattct caccctcggc
    13021 accgtggcgg gcatcaccgc cgtccgaacc ctggagcaaa gccggaagga cgccgtggac
    13081 ctgctcgagg cactctccaa ggaacatgcc gcggagatca acgtggaact cgaggtcgcc
    13141 atggacgcgg cgcgcaccct ggcccagatc ttcgaagggt acgagagcct ccatgcggac
    13201 gagcggcggg aggactacag caagatgctc cgatccatcg tggagaagaa ccccgggttc
    13261 ctgggggctt ccacgggatg ggagccgaac gccctggacg gccgcgatcg ggaattcgtg
    13321 gacgctccgg gccacgacgg gacgggccgg ttcatcccct actggagtcg ttttgagggc
    13381 cccgtggagc tgtcccccct ggtcgactac gataaggagg gggccgggga ctattacatt
    13441 cttcc
//
LOCUS       DAOTEI010000091        13381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3212_length_13381_cov_4.402822, whole genome shotgun sequence.
ACCESSION   DAOTEI010000091 DAOTEI010000000
VERSION     DAOTEI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13381
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3212_length_13381_cov_4.402822"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="P5313_09780"
     CDS             complement(<1..677)
                     /locus_tag="P5313_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRY80695.1"
                     /translation="MEEVFERLRQFQDILKERVEIEREIENIPKSLVAQEELLARVKK
                     GYAEKTEQLQAVTARVAELRNELSEAEASRERAEKLMDTISTQREYEALNKEIRDATD
                     KEHQVRKDLQKEERALAEIEESFKRDESMIQLQESEILEKKQKISEETAQKQTRVSEL
                     RASEKTATEGIDTEITFKFERIIRSKQGLGIVPVRGYVCSGCSMILPAQFVNEVRQGN
                     RIIFCPY"
     gene            complement(746..2461)
                     /gene="rpoD"
                     /locus_tag="P5313_09785"
     CDS             complement(746..2461)
                     /gene="rpoD"
                     /locus_tag="P5313_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRY80696.1"
                     /translation="MSDLELDPAVIKLLEYAKSKNSITFDELSDFLPEHVLNTDKIDV
                     VLALLESHNIRLEEEESPADEEAAAKEIESSKKRLVYTDKESAVDDPIRLYLREIGRE
                     NLLTAEEEIKLSQQMEEGEGIIKGIIRTSGMLIPEFYQISQKAKRESGPQRKENTEKI
                     AERRRLNQFYRDVIRDTSAELRAYVEAKRRILSRGGDALKDEGLDDSRKTLLEKLRDV
                     EIHPEEINSFSEKFILAAKKIRKFRKEQERLEKTLQVSNLRELRALGRNLTIRDRRDK
                     IEEEIGLSSDEIKERIRQIQVTEKRLDELENSFECGCDIILSMAKEINRGRIMMKNAK
                     DRLIKANLRLVVSIAKKYTNRGLHFFDLVQEGNIGLIKAVEKFEYRKGFKFSTYATWW
                     IRQAITRSISDQARTIRVPVHMIEQINKVVRESRQLMQKLGREPTDEEVAQQLGWPVS
                     RVKSVKNVAREPISLETPIGEDEDSLLGDFIEDKEIENPSVQTDYKLLQEQIRSVLST
                     LPPREQEVLRMRFGLDDGYSLTLEEVGLYFNVTRERIRQIEAKALKKLRHFKRSQKLR
                     DYMDH"
     gene            complement(2448..4250)
                     /gene="dnaG"
                     /locus_tag="P5313_09790"
     CDS             complement(2448..4250)
                     /gene="dnaG"
                     /locus_tag="P5313_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRY80697.1"
                     /translation="MKRIRKDSLDTVLGRTDLVAVIGDYVRLQRSGSGYKGLCPFHNE
                     KTPSFNVVPEKGLFYCFGCQKGGDVIAFLREIEGLSFVEAVERLAEKSGVQLEYEGSD
                     GSPDDGARESRERDSLYELYERLTSAFQWLLREHPSGKAAREYLARRKVSRETLDSFR
                     LGYAPRDRRWLFEFLRKKGYSPEFLAGTGLFSRDRPDRAFFADRVIFPITDYRGRSVA
                     FGGRILEGDGPKYLNSPESRIFHKQDNLFALSAAAKAIRQDRTALICEGYMDVLAFHQ
                     AGIGYAVAPLGTAFTDGQARLLRRYADQAVFCFDSDEAGGRATEKALMTAARNGLRAK
                     ILVLEGGKDPAEILETRGGEELKKLSTFTINGDDFVVRRARTARDTTTAEGKALALKS
                     LFPYMSALDSEIRLAEFADFAAREFRSKTDRVLADFEKFRSSGGRETGASGPESRVPA
                     LRPTSDLAFLAAAALNPGQFAAVRSRMEAADLDDEASRELFLALEECFRNDETDLDDL
                     LARVSNPGLRNFLMEKAANGEYAIQVDRLIRDGILNVRERSLERRRSRLVRQIAQYDT
                     DREADSVSVNDLLYEKMFIDQELEKIKEERNERS"
     gene            complement(4247..5311)
                     /gene="mltG"
                     /locus_tag="P5313_09795"
     CDS             complement(4247..5311)
                     /gene="mltG"
                     /locus_tag="P5313_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRY80698.1"
                     /translation="MAARSSPKPALKALLLLPLFLAGIGILLAAGAVAVFAVLDAPPE
                     GMPPEGALFTVEAGEPGARIAVRLEDAGLIRSALAFQALLRLRNQQTSLKRGTYRIEP
                     GMGGRDVQELLVSGRQALVRVTVPEGHTLRRSAEVLHEAAVTDREDFLAAARDPDLLA
                     SLGIPAATAEGYLFPDTYYLPLKTPGAEAVRLMVGALRSRLAEGLPESLSLDPRELHR
                     RIILASVVEREYRLPEEAPRIAGVFWNRLRIGMALQSCATVVYIITEVQGKKHPEVLY
                     NRDIEIQHPFNTYLHPGLPPAPIANPGLTALQAVFRPEPSRYLYFRLLDARTGRHYFS
                     ETLDEHIRAGSLAVKGIGGR"
     gene            complement(5319..5537)
                     /locus_tag="P5313_09800"
     CDS             complement(5319..5537)
                     /locus_tag="P5313_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80699.1"
                     /translation="MTILDPYDLRLSPRETIELYRFLKRAEDGMPREVSGVLEKLRER
                     LYSRLSIEEMENIEVYLGILTAAEAAHR"
     gene            complement(5539..6477)
                     /locus_tag="P5313_09805"
     CDS             complement(5539..6477)
                     /locus_tag="P5313_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY80700.1"
                     /translation="MKIRFWGVRGSIPTPLDSDAIRSKISAVIQRVSRKDIESPESRE
                     RFLASLPDEIFSTVGGNTSCVEVLASREERIIFDAGTGIRDLGAEIAASSGAARCHVF
                     FSHFHWDHVQGLPFFAPAFNPSSTITFYSPEPGLKEILAGQMRPPYFPVTMDVMRASL
                     RFERIGTEGVRLGGVRVVPRRMNHPGGCWSYSVLSDGKKVVYSTDTELQPVDFERNPE
                     NAAYFRGADALIIDAQYTLGEAIEKFNWGHSSFSLAADFASTWGIKRLVLFHHEPSYD
                     DRKIHKLLQSAEWYVERLASKGLEVILARDGLTVEV"
     gene            complement(6452..6940)
                     /locus_tag="P5313_09810"
     CDS             complement(6452..6940)
                     /locus_tag="P5313_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRY80701.1"
                     /translation="MVSDVHGAGGLPFECTRCSACCRHEPGLVLLSREDLRRLLGRLG
                     LDFGAFYAKYCRLVPLGGGVAISLRETKEYDCIFWKSGCTVYDARPVQCRTYPFWPGI
                     VDSRTDWIREGRRCPGIGRGPIRNRSEIEEALWEYRRNSPILLDPGTDPEHLDENTIL
                     GS"
     gene            complement(6987..7068)
                     /locus_tag="P5313_09815"
     tRNA            complement(6987..7068)
                     /locus_tag="P5313_09815"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7032..7034),aa:Tyr,seq:gta)
     gene            complement(7098..7170)
                     /locus_tag="P5313_09820"
     tRNA            complement(7098..7170)
                     /locus_tag="P5313_09820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7135..7137),aa:Thr,seq:tgt)
     gene            complement(7293..9374)
                     /locus_tag="P5313_09825"
     CDS             complement(7293..9374)
                     /locus_tag="P5313_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80702.1"
                     /translation="MKHATALVAFVLAAAAASAQFPYGLSDPSAQENFRLGVQAYHRG
                     RTPESILLFERVLSFNPRDPLSLFWLGRAYFKNGLESTALDRWEAAAREGGRSLLIES
                     FQEFVSSRRAVEPGMGRGGYVSAGEIPGLQGRETRFLRPSWIHPLRDGGFLLVSHGTG
                     EILRVDVNGVVRARFRGGVGGFDRPFSLAPAPDGGFWVSEFQGDRISRCDKDGNVLSR
                     IGGGKGRERLTGPQYLASDSGGFLFAVDFGNGRVVKYSPSGEFLLEFGRSSPVFPGFR
                     SPTGIAVRDGKVYVADAFRKAIYVFDDSGNYLESFAEGSLLGPEGMTFAGNGELLVAD
                     RRRVLAADTTTYEIRELYRSPRPAARLVSATLDANGNVLAADYDASSVDVLSDPAVVY
                     GGYFLEIERIRADSFPVVEADVYVRDRFGNPVVGLDQGNFYASEQISVERSVKEGETE
                     ARIRTETLTPVRELELVGRLSDTPGVSVALVVERSPGIDAYRSEVRQAVESVASGLGD
                     PSEAFLVTAGRVPALQGTASGSSGGPVLARQAISAEVDPLWRFDAALRLAVSTLTERL
                     GRSAVVYITTGTLNEARFEGPTLSELGTYLRNNGIRFYAVVVGDAPVSEAVRYLAGTA
                     GGGVYRVWEPAGLGRIGRDLQDAASGRYRIRFTSAADPGFGRSYLRIAVEAYLFRKSG
                     RDESGYFAPLR"
     gene            complement(9371..10045)
                     /locus_tag="P5313_09830"
     CDS             complement(9371..10045)
                     /locus_tag="P5313_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY80703.1"
                     /translation="MRSEIREALRSYSEGDWEEALRLLSGAELSEDEFGDSAYLLGLC
                     HVRLGNFDEALLYLEQVVTAETDPARLYQCRMALAYIYAVTGRVKLAEYELARLRESG
                     YESAQICAALGYTAWVQGNIDDSLQWYGRALDLEPAHPTSLNGLGYVLAYRGDDLTRA
                     LSCCRKAVEARPNNPVYQDSLGWVYLKLGFLDEAETWLERALKGAPEEREVQEHVRTL
                     RELKTL"
     gene            complement(10072..10809)
                     /gene="surE"
                     /locus_tag="P5313_09835"
     CDS             complement(10072..10809)
                     /gene="surE"
                     /locus_tag="P5313_09835"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRY80704.1"
                     /translation="MKILVTNDDGIHCEGLRVLAEELGRDHEVWVFSPDGDRSGSSHS
                     MTLRSPGKVRALDERTYTCSGMPADCVILAFRGAIPFRPELVVSGINRGPNLGTDIVY
                     SGTAAAARQAVLHGIPGIAVSLASYGPAFDFRPAARFVRSNLGALLAAWDASVFLNVN
                     VPPGPDADTRGAVFTRPGKRTYRDSLHTFEAPDGFTYCFLTGETIDNAGEEAADTSAV
                     DSGLIAVTKVLVDPQVPPAFPAGQRFP"
     gene            complement(10888..12033)
                     /locus_tag="P5313_09840"
     CDS             complement(10888..12033)
                     /locus_tag="P5313_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HRY80705.1"
                     /translation="MKDIAELHTQEFEAPPEVIASAPGTLRLLGEHTEDSEGVVLSIA
                     LSQRIAVAVSPRRDSSLRFYAADLNERKRTSLANLKFKREDRWANLPKGILAALFDQG
                     APAQGFNVTVAGDIPQGIGLGSSSAFVVASAAAFSGALGKNLGNDGLVELSRVAEAEF
                     CGRTFGPAEHVVPVVSRPGTAVSVDARNRRPRLVPFDIGRSVALITDSRVPRFSVDAE
                     LRQRGDECRQCLHQLGRRHPGKTLRDYTPEDLDELMGELPESIRRRCLFILEEVRRVS
                     EAEDLLARGDLVGFGRVVNKSQAGLRNLLEVSCPEVDWLVKRSSEIEGTLCSRLTGKG
                     FGGCTITLLSRDALEEYKNRLEEYERIFGFKAAIYETSVSGGIELQG"
     gene            12210..12755
                     /locus_tag="P5313_09845"
     CDS             12210..12755
                     /locus_tag="P5313_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80706.1"
                     /translation="MRTVRILLAPLVAAALILSCAGTPEEPVPLPGDPPPAAVTPPPP
                     PPGQAFDPASITPEVKRDTMEEVKSFINVVNQVIRQKDYTVWRGYLTPEYSTFYSSPA
                     VLAEMSESAVLKRQGIKLESLQDYFLYVVYPSRQSVRVDDIEFTSPTQVKAVTITPSG
                     DRQVYYYLEKVGESWKIGTGR"
     gene            12758..>13381
                     /locus_tag="P5313_09850"
     CDS             12758..>13381
                     /locus_tag="P5313_09850"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY80707.1"
                     /translation="MRSLRSAALAALLVVALAGVAAQTTGSGEKTVEEAYLQETLETM
                     IIKEQARGDTRDLKLLALQYIKQSIDEGRKNEEVRKSLEYLALETSTIVVREAGVGRI
                     INDYPDVRAKACEYLAEFPTVETKDTLLRVALSDREPMVISAAIRSLGKIGINDNDEV
                     TQTIAFIVNKYDILQPDNSLAFETLVAIERLVDAGTGIKDPSIIRTVL"
BASE COUNT         2339 a         4344 c         4270 g         2428 t
ORIGIN      
        1 cagtaggggc agaagatgat ccggttgccc tgccggacct cgttgacgaa ctgggcgggc
       61 agtatcatgg aacagccgga gcacacgtag ccgcggacgg ggacgatgcc caggccctgc
      121 ttcgaccgga tgatcctctc gaatttgaag gtgatctcgg tgtcgatccc ctcggtggcg
      181 gtcttctcgg aggcccggag ctcggagacc cgggtctgct tctgggcggt ctcttcggag
      241 atcttctgtt tcttctccag gatctcggat tcctggagct ggatcatcga ttcgtcccgc
      301 ttgaaggatt cctcgatctc cgccagggcg cgttcctcct tctggaggtc cttgcgcacc
      361 tggtgctcct tgtccgtggc atcccggatt tccttgttca aggcctcgta ttcccgctgg
      421 gtggagatgg tgtccatgag cttctcggcc cgctcccggg aagcctcggc ctcggacagt
      481 tcgttgcgga gctcggccac ccgggcggtg acggcctgga gctgctcggt cttctcggcg
      541 tatcccttct tgacgcgggc caggagctcc tcctgggcca ccagggactt ggggatgttc
      601 tcgatctctc tctcgatctc gacgcgttcc ttgaggatgt cctggaattg ccggagccgc
      661 tcgaaaacct cttccatcaa catagggaat cacctcgtag ttagttgtcc aagtccgcaa
      721 cgatagcacg gaacgcgctt ggtcatcaat gatccatgta gtcccggagt ttctggctcc
      781 gcttgaagtg ccggagcttc ttgagcgcct tggcctcgat ctgccggatc cgctcccgcg
      841 tgacgttgaa gtacagcccc acctcttcca gggtcaggga atacccgtcg tccaggccga
      901 agcgcattcg gaggacctcc tgctcacggg gcggcagggt cgagaggacg gagcggatct
      961 gctcctgcag gagcttgtag tccgtctgga ccgagggatt ctcgatctcc ttgtcctcga
     1021 tgaaatcccc gagcagggaa tcttcgtcct cgccgatggg ggtttccagg gaaatgggct
     1081 cccgggccac gttcttgacg ctcttgaccc ggctgacggg ccacccgagc tgctgagcca
     1141 cttcctcgtc cgtgggctcc cggccgagct tctgcatgag ctggcgggat tcccgcacca
     1201 ccttgttgat ctgctcgatc atgtggaccg ggacgcggat agtccgggcc tggtcgctga
     1261 tggagcgggt gatggcctgc cggatccacc aggtggcgta ggtggagaac ttgaacccct
     1321 tgcggtactc gaacttctcc accgccttga tgaggcctat gttcccctcc tggacaaggt
     1381 cgaagaagtg gaggccccgg ttcgtgtact tcttggcgat ggagaccacc agccggaggt
     1441 tcgccttgat gaggcggtcc ttggcgttct tcatcatgat ccggccgcgg ttgatctcct
     1501 tggccatgga caggatgatg tcgcatccgc actcgaagga gttctcgagc tcgtccagcc
     1561 gcttttccgt gacctggatc tgccggatcc tctccttgat ctcgtccgag gacagcccga
     1621 tctcctcctc gatcttgtcc cggcggtccc ggatggtcag gttccggccc agggcccgaa
     1681 gctcccggag gttggaaacc tgaagggtct tctccagccg ctcctgctcc ttgcggaact
     1741 tgcggatctt tttggccgcc aggatgaact tctcggagaa ggagttgatc tcctcggggt
     1801 ggatttccac gtcccggagc ttctccagga gggtttttcg cgaatcatcc agtccctcgt
     1861 ccttgagggc gtcgccgccc cgggacagga tgcgccgctt ggcctccaca taggcgcgaa
     1921 gttcggccga ggtgtcccgg atgacgtcgc ggtagaactg gttgagccgc ctccgttccg
     1981 cgatcttttc cgtgttctcc ttgcgctggg ggccggattc ccgcttggct ttctgggata
     2041 tctggtagaa ctcggggatg agcatgccgc tggtgcggat gatgcccttg atgatcccct
     2101 cgccctcctc catctgctgg gagagcttga tctcctcctc ggccgtgagc aggttctccc
     2161 ggccgatttc ccggaggtag agccggatgg gatcgtcgac cgcggattcc ttgtccgtgt
     2221 agaccaggcg cttcttggag gattcgatct ccttggccgc cgcctcctcg tccgcggggc
     2281 tttcctcctc ctcgagccgt atgttgtggc tttccaggag ggcaaggacg acgtctatct
     2341 tgtccgtgtt gagcacgtgc tccggcagga aatccgaaag ctcatcgaag gtgatggaat
     2401 tcttcgattt tgcgtactcg agtagcttga ttaccgcggg atcgagttca agatcgctca
     2461 ttccgctctt cctttatctt ttccagctct tggtcgatga acatcttctc gtacaagagg
     2521 tcgttcaccg ataccgagtc cgcttcccga tccgtatcgt actgggctat ctgccgaacc
     2581 agccgggacc tgcgccgttc caggctgcgc tcccgcacgt tcaggatgcc gtcccggatc
     2641 aggcgatcga cctggatcgc gtattctccg ttggcggcct tctccataag gaaattccgc
     2701 aggcccggat tggatacgcg ggccagcaga tcgtccaggt ccgtctcgtc gttccggaaa
     2761 cactcctcca gggccaggaa cagctcccgg gaagcctcgt catccaggtc cgccgcctcc
     2821 atgcgggatc gtaccgccgc gaactggccg gggtttaggg cagccgcggc caggaaggcc
     2881 aggtccgaag tcggccgcag ggcgggcacc cgactttccg ggccgctcgc tccggtctcc
     2941 cgcccgccgg aggaccggaa tttctcgaaa tccgccagga cacggtccgt cttgctgcgg
     3001 aattctcggg ccgcgaagtc ggcgaactcc gccaggcgga tctccgaatc cagagcggac
     3061 atgtacggaa acaacgattt cagggcgagg gccttccctt cggcagtggt agtatcccgc
     3121 gccgttcggg cccggcgcac gacaaaatcg tctccattta tagtgaaagt cgaaagtttt
     3181 ttcaattcct ccccgccccg ggtctccaga atttccgcgg ggtcctttcc gccctccagg
     3241 acgaggatct tggcgcgcag gccgttccgc gcggcggtca tgagtgcctt ctccgtcgcc
     3301 cggccgcccg cctcgtcgga atcgaagcag aacaccgcct ggtccgcata tctgcgcaaa
     3361 agacgggcct gtccgtccgt aaaggccgtt cccaggggag ctacggcata cccgatccct
     3421 gcctggtgga aggccagcac gtccatgtac ccctcgcaga tcagggccgt ccggtcctga
     3481 cggatcgcct tggccgccgc ggacagggcg aagaggttgt cctgcttgtg gaaaattcgg
     3541 ctttccgggg agttgaggta cttcgggccg tccccctcca ggatccggcc gccgaaggcc
     3601 acgctccggc cccggtagtc ggtgatcggg aatatcacgc gatccgcgaa aaaggcccga
     3661 tccgggcgat cccgggaaaa caaccccgtt ccggcgagga attccgggga ataccccttc
     3721 ttccggagga attcgaagag ccacctgcgg tcccgggggg cgtatcccag ccggaaagaa
     3781 tccagggttt cccgagagac cttcctgcgg gccaggtatt cccgggcggc cttgccggaa
     3841 ggatgttccc ggaggagcca ctggaaggcc gaggtgaggc gctcgtacag ctcgtacagg
     3901 gaatcccgct cccgggattc ccgggccccg tcgtccgggg agccgtcgga tccctcgtat
     3961 tccagctgga cgccggattt ctcggccagg cgctccacgg cctccacgaa ggacagtccc
     4021 tcgatctccc ggagaaaggc gatcacgtcg ccgcccttct ggcagccgaa gcaatagaac
     4081 agccccttct caggcactac gttgaacgaa ggcgtcttct cgttgtggaa ggggcacagg
     4141 cccttatatc ccgatcccga cctctggaga cggacatagt ccccgatcac ggcgaccagg
     4201 tccgtccttc ccaggacggt atccagggaa tccttgcgta tgcgcttcat cgtcccccga
     4261 tgcccttcac ggcaagggat cccgcccgga tatgctcgtc cagggtttcg gagaagtagt
     4321 gccggcccgt gcgggcgtcc aggaggcgga agtagaggta tcgggagggc tccgggcgga
     4381 agacggcctg gagggccgtc agtcccgggt tggcgatggg ggccggagga agacccgggt
     4441 ggagataggt gttgaaggga tgctggatct cgatatcccg gttgtacagg acttcgggat
     4501 gcttctttcc ctggacctcg gtgatgatgt acaccactgt ggcgcaggac tgcagggcca
     4561 ttccgattct caggcggttc cagaacacgc cggcgatacg gggggcctcc tccgggagcc
     4621 tgtattccct ctccaccacg gatgccagga tgatccgccg gtgcagttcc ctggggtcga
     4681 gggagaggga ttccggcagg ccttccgcca aacgggaccg gagcgctccg accatcagcc
     4741 gtacggcctc ggcgcccggg gtcttcaggg gaagatagta ggtgtccggg aagaggtatc
     4801 cttccgcggt cgccgcgggt atgcccaggg acgccaggag gtcgggatcc cgggcggcgg
     4861 ccaggaaatc ctcccgatcc gtcaccgctg cctcgtgcag gacctccgcg gagcggcgca
     4921 gggtatgacc ctccggaacg gtcacccgga ccagggcctg cctgccggac accaggagct
     4981 cctggacatc ccggccgccc atcccgggct cgatccggta ggtcccccgt ttaagggagg
     5041 tttgctggtt tcgaagccgg agcagggcct ggaaggccag ggcggatcgg atcaatcccg
     5101 cgtcctccag gcggacggct atccgcgccc ccggctcccc ggcctccacg gtgaagaggg
     5161 ccccttccgg gggcatcccc tccggggggg cgtccaggac tgcgaagacc gccacggctc
     5221 ccgcggccag caggatcccg atccccgcca ggaagagggg aagcagcagg agggctttga
     5281 gcgcgggctt gggcgaggat cgtgcagcca tgggtttcct atctgtgcgc ggcttccgcc
     5341 gccgtcagga tgccgagata gacttcgata ttctccatct cctcgatgga aagacgggaa
     5401 tacagccgct cccggagttt ttcaagaacc ccggacacct cccggggcat gccgtcttcc
     5461 gccctcttca ggaagcggta cagttcgatc gtctcccgcg ggctcaggcg caggtcgtag
     5521 ggatccagga tcgtcacgtc agacctctac cgtaagcccg tcccgggcca ggatgacctc
     5581 gagccccttg gacgcgaggc gttcgacgta ccactccgcg gactgcagga gcttgtggat
     5641 cttgcggtca tcgtacgagg gctcgtggtg gaaaagaacc aatcgcttga tcccccaggt
     5701 ggacgcgaaa tccgccgcca ggctgaagga cgagtgtccc cagttgaatt tctcgatggc
     5761 ttcccccagg gtgtactggg cgtcgatgat cagggcgtcc gccccccgga agtacgccgc
     5821 gttctcgggg ttgcgctcga aatccacggg ctggagctcc gtgtccgtag aatatactac
     5881 cttcttgccg tcggagagta ccgaatagct ccagcaaccc ccgggatggt tcatcctccg
     5941 gggcacgacc cggactcctc ccaggcgtac accctcggtc ccgatccgct cgaatcggag
     6001 ggaggcccgc atgacgtcca tggtgaccgg gaaatagggg gggcgcatct gcccggccag
     6061 gatctccttc aggcccggtt cggggctgta gaatgttatc gtggaggatg gattgaaggc
     6121 gggggcgaag aagggcaatc cctggacgtg gtcccaatgg aagtgggaga agaagacatg
     6181 gcaccgggcg gccccgctgg aggccgctat ttctgcgccc aggtcgcgga tgccggtgcc
     6241 cgcgtcgaag atgatcctct cctcccggga agcgaggacc tcgacgcagg aggtattccc
     6301 cccgacggtg gagaatatct cgtccggaag ggaggccagg aaccgttccc ggctttccgg
     6361 actctcgatg tccttgcggg agacccgttg aatcaccgcg gagatcttgc tgcgaatggc
     6421 gtcggaatcc aggggtgtcg gaatggatcc cctaactccc cagaatcgta ttttcatcca
     6481 ggtgctccgg atccgttccg ggatcgagaa ggatcggcga gttcctgcgg tattcccata
     6541 gagcctcctc gatttccgac cgattccgaa tcggacccct tcctatgccc gggcagcgcc
     6601 gtccttcccg gatccagtcc gtccgggaat cgacgatccc gggccagaac ggataggtac
     6661 ggcattggac gggacgggca tcgtacacgg tacagccgct tttccagaat atgcagtcgt
     6721 attccttggt ttcccggagg ctgatcgcga cccccccgcc caggggcacc agcctacagt
     6781 atttggcata gaacgcgccg aaatcaaggc ccagacgtcc caggaggcgc ctaaggtcct
     6841 cccgggacag caggaccaga ccgggttcgt ggcggcagca tgcggaacag cgggtgcatt
     6901 cgaagggaag gcccccggcc ccgtgaacat cggacaccat ttggacctcc gggcaagaaa
     6961 aaaccctctc gaccgtgaga gggttctggg gagagaagga tttgaacctt cgaaggcata
     7021 gccaacagat ttacagtctg ccccctttga ccactcggga atctccccct gtgaagcacg
     7081 ccgtccgtca gggctcgagc catctcccgg aatcgaaccg gggacctcga gattacaagt
     7141 cacgcgctct accgactgag ctaagatggc gttttcttcc cgtttcgaga acggccggac
     7201 cgggtccgga ccgtcgtcgg aacgagtcct accctaccat gagcggctcg tccttgtcca
     7261 ccccggggcg tagcggaccg gtcgaaaaat cgtcacctaa ggggggcgaa gtacccggac
     7321 tcgtcccggc cggacttccg gaagaggtag gcctccacgg cgatccggag gtaggacctc
     7381 ccgaagcccg ggtccgccgc gctggtgaag cggatccggt accgcccgga cgcggcgtct
     7441 tgcaggtcgc ggcctatccg tccgaggccc gcgggctccc atacccggta taccccaccg
     7501 cccgccgtcc cggccaagta ccgcacggct tcggaaaccg gggcgtcccc cacgaccacg
     7561 gcgtagaagc ggatgccgtt gttgcgaagg taggtcccga gttccgagag ggtcggaccc
     7621 tcgaagcggg cttcgttcag ggttcccgtg gtgatgtaga ccaccgccga acggcccagc
     7681 cgctccgtca gggtggagac cgccagccga agggccgcgt cgaacctcca cagcgggtcg
     7741 acctcggcgg agatggcctg tcgggccagg acggggcctc cggagcttcc ggaggccgtc
     7801 ccctgcagtg cgggtaccct tcccgcggtg accaggaagg cctcggacgg atcgcccagt
     7861 cccgaggcca cggactccac ggcctgtcgg acctcggatc gataggcatc gatcccgggg
     7921 gatcgctcca cgacgagggc tacggagact ccgggggtat cggacaagcg cccgaccaac
     7981 tcgagttccc gcacgggagt cagggtttcc gtacggatcc gggcttcggt ctccccctcc
     8041 ttgacggacc gctccacgga aatctgctcg gaggcgtaga agttgccctg gtccaggccg
     8101 accacgggat tgccgaaccg gtcgcggacg tagacgtccg cttccacgac ggggaacgag
     8161 tccgcgcgga tccgctcgat ctccaggaaa tacccgccgt agaccaccgc gggatccgac
     8221 aggacgtcca cggaggaggc gtcgtaatcc gcggccagca cgttcccgtt ggcgtccagg
     8281 gtggcggaga cgagccgggc cgcgggccgg ggcgaacggt agagctcccg gatctcgtag
     8341 gtggtcgtat ccgcggccaa cacgcggcgc cggtccgcga ccaggagttc gccgttcccc
     8401 gcaaaggtca ttccctcggg gcccaggagg cttccttccg cgaaggattc caggtagttc
     8461 ccggagtcgt cgaagacata gatcgccttg cggaaggcgt ccgccacgta gaccttcccg
     8521 tcccgaacgg ctatgcccgt cggggaccgg aaacccggaa agaccgggga agagcgcccg
     8581 aactccagga ggaattcccc ggacggggaa tacttgacga cccgcccgtt cccgaagtcc
     8641 acggcgaaca ggaatccgcc cgaatccgag gccaggtact ggggccccgt cagccgttcc
     8701 ctgcccttcc cgccgccgat gcgggaaagg acgttcccgt ccttgtcgca gcgggagatg
     8761 cggtcgccct ggaactcgga tacccagaat cccccgtcgg gcgcaggcgc cagggaaaag
     8821 ggccgatcga acccgccgac gccaccccgg aaacgggctc ggaccacccc gttcacgtcc
     8881 acccggagta tttctcccgt gccgtgggag accaggagga acccgccgtc ccgcaggggg
     8941 tggatccagg atggacgcag gaaccgggtc tccctgccct gcaggcccgg gatttccccc
     9001 gcggagacgt agcccccgcg ccccatgccg ggttcgaccg cccgacggga ggagacgaat
     9061 tcctggaagc tctcgatcag gagggaacgg cctccctccc gggccgccgc ctcccatcga
     9121 tccagggctg tgctttcgag cccgttcttg aagtaggccc ttcccagcca gaacagcgac
     9181 aggggatccc ggggattgaa ggaaagcacc cgctcgaaca ggaggatgga ttccggggtc
     9241 cggcctcgat ggtacgcctg gacgcccagc cggaaattct cctgggccga ggggtcggaa
     9301 aggccgtagg ggaactgcgc cgatgccgcc gcggcggcca ggacgaatgc gacgagtgcg
     9361 gttgcgtgct tcatagggtc ttcagctccc tgagtgttcg aacgtgctcc tggacttccc
     9421 gctcctccgg ggcgcccttc aaggcgcgct ccagccaggt ctccgcctcg tccaggaatc
     9481 ccagcttcaa gtagacccag cccaggctgt cctggtagac ggggttgttg ggccgggcct
     9541 ccacggcctt gcggcagcag gacagggcgc gggtcaggtc gtccccccgg taggccagga
     9601 cgtatcccag accgttgagg gaggtcggat gggcgggttc gagatccagg gcccggccgt
     9661 accattgaag actgtcgtcg atgttcccct gcacccaggc ggtatatccc agggccgcgc
     9721 agatctgggc ggactcgtac ccggattccc gcaggcgggc cagctcgtac tccgccagtt
     9781 tcacccgtcc ggtcacggcg tagatgtagg ccagggccat gcggcactgg tagagccggg
     9841 ctggatccgt ctcggccgtg accacctgct ccaggtagag cagggcttcg tcgaagttcc
     9901 cgaggcggac atggcacaat cccaggaggt aggcggaatc cccgaactcg tcctccgaaa
     9961 gctccgcgcc cgacaggagc cgtagggctt cctcccaatc gccttccgag taggatcgca
    10021 gggcttcccg gatctcgctg cgcatcgggt ccgccttcct tcccgggttc ctcaggggaa
    10081 cctttggccc gccggaaaag ccgggggcac ctggggatcc acgagcacct tggtcacggc
    10141 gatgagtccg gaatcgaccg cgctcgtatc cgcggcttcc tccccggcgt tgtcgatggt
    10201 ctcgccggta aggaagcaat aggtaaagcc gtccggagcc tcgaaggtat ggagggagtc
    10261 ccggtaggtc cgcttgcccg gacgggtgaa gaccgccccc cgggtatccg cgtccggccc
    10321 cgggggcacg tttacgttca ggaagacgga ggcgtcccag gccgccagca gggcgcccag
    10381 gttcgagcgg acgaagcggg ccgcggggcg gaaatcgaag gcgggaccgt agctcgccag
    10441 ggagacggcg attcccggta ttccgtggag gaccgcctgg cgggcggccg cggcggtgcc
    10501 ggaatagacg atatccgtgc ccaggttggg gccccggttg atccccgaca ccacgagctc
    10561 cgggcggaac ggaatggctc cccggaaggc caggatgacg cagtccgcgg gcatgccgct
    10621 gcaggtatag gtcctctcgt ccaaggcgcg gaccttgccg ggggatcgca gggtcatgga
    10681 atggctggaa ccggacctgt caccgtcggg tgagaacacc cagacctcgt ggtccctccc
    10741 gagctcctcc gccaggaccc ggagaccctc gcagtggatt ccgtcgtcgt tcgtaacgag
    10801 gatcttcatg gtttcgacaa gggccccctc ccgcgtcccg tcaggttgac cctgacaagg
    10861 ggattgtagc ggaagcgggg gaaggattca accttgaagc tcgatgccgc cggagacgga
    10921 ggtctcgtag atcgccgcct tgaatccgaa gatccgctcg tattcctcca ggcggttctt
    10981 gtactcctcc agggcgtcgc ggctgagcag ggtgatggtg cagcccccga agcccttgcc
    11041 ggtcagccgg ctgcaaaggg tgccctcgat ctcggaactt cgcttgacca gccagtccac
    11101 ctcggggcag gagacctcca ggaggttccg gagccccgcc tgggacttgt tcacgaccct
    11161 cccgaatccg accaggtccc cccgggcgag caggtcctcc gcctcggaga cccgcctcac
    11221 ctcctccagg atgaagaggc agcggcggcg tatgctttcc gggagttccc ccatgagctc
    11281 gtccaagtcc tcgggggtgt agtcccggag ggtcttgccg ggatgtcggc gccccagctg
    11341 gtgcaggcat tgacggcatt cgtccccccg ctgtcggagc tccgcgtcca cggaaaaccg
    11401 gggaacccgg gaatccgtga tcaaggcgac ggaacggccg atgtcgaagg ggaccagccg
    11461 gggacgtcga ttccgggcgt ccacggacac ggccgtgccg gggcgcgata ccaccggcac
    11521 cacgtgctcg gcggggccga aggtacggcc gcagaattcg gcctccgcga cccgggagag
    11581 ctccacgagc ccgtcgttcc cgaggttctt cccgagagcc ccggaaaaag cggcggcgga
    11641 agccaccacg aaggcggagg aggaacccag gcctatgccc tggggtatgt cgccggcgac
    11701 ggtgacgtta aacccctggg ccggggctcc ctggtcgaac agggccgcca ggatcccctt
    11761 gggaagattg gcccagcggt cctcgcgctt gaacttgagg ttcgccaggc tcgtccgttt
    11821 ccgctcgttc aggtccgcag cgtagaaccg aagggaagaa tcgcgtctgg gggagacggc
    11881 tacagcgatg cgctgggaca gggcgatgga aaggaccacg ccttcggaat cttccgtgtg
    11941 ctcacccagc aaccgcaacg ttcccggagc ggacgcgatg acttcggggg gagcctcaaa
    12001 ctcctgggta tggagttccg ctatatcctt catcgaccag ccttgctatc gaacgaaata
    12061 atgctattgc gagatcatga tatactatga acgcggaagg ttcaaacgaa aaattcgcca
    12121 aaaccgggtc tgtcctcgct catcccggga ttccgtcaat tcgacgccgt ttctttgaca
    12181 attcccggcg ccggaagtag aatcacagca tgcgaaccgt ccggatcctt cttgcacctt
    12241 tggttgcggc cgccttgatc ctgtcctgcg ccgggacgcc cgaggagccc gtcccccttc
    12301 ccggggatcc ccctccggcc gccgtcacgc cccctccccc gcccccggga caggccttcg
    12361 atccggccag catcaccccc gaggtcaagc gggacactat ggaggaagtg aaatccttca
    12421 tcaacgtggt caaccaggtc atccgccaga aggactacac cgtgtggcgg ggctacctga
    12481 caccggagta ttccaccttc tactcgtccc ccgccgtcct ggcggaaatg tccgaatcgg
    12541 ccgtgctgaa acggcagggg atcaaactcg agtcccttca ggattacttc ctgtacgtcg
    12601 tatacccctc ccggcagagc gtccgggtgg acgatatcga gtttaccagt ccgacccagg
    12661 tcaaggccgt gaccatcacc ccttcggggg atcgccaagt atattactac cttgaaaaag
    12721 taggcgaatc gtggaaaatt ggtaccggga ggtaatgatg cgttcattgc gttcggcggc
    12781 cctggccgcg ttgctcgtgg tagccctggc gggcgtcgcc gcccaaacga cgggatccgg
    12841 ggagaagacc gtggaggagg cgtacctcca ggaaaccctg gaaaccatga tcatcaagga
    12901 gcaggcccgg ggagacaccc gagatctcaa gctgctcgcc ctccagtaca tcaagcagtc
    12961 catcgacgag ggacggaaga acgaggaagt ccgcaaatcc ctcgagtacc tcgccctgga
    13021 gacctcgacc atcgtggttc gcgaggccgg cgtcggtcgg atcatcaacg attacccgga
    13081 tgtccgcgcc aaggcctgcg aatacctcgc cgagttcccc accgtggaga ccaaggatac
    13141 cctgttgcgc gtcgccctct ccgaccggga gcccatggtc atcagcgcgg cgattcggtc
    13201 cctgggcaag atcgggatca acgacaacga cgaggtgacc cagaccatcg cgttcatcgt
    13261 caacaagtac gacatcctcc agcccgacaa cagcctggcc ttcgagaccc tggtcgcgat
    13321 cgaacggctg gtggacgccg gaaccggaat caaggacccc tcgatcatcc ggaccgtgct
    13381 t
//
LOCUS       DAOTEI010000092        13228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3254_length_13228_cov_4.958020, whole genome shotgun sequence.
ACCESSION   DAOTEI010000092 DAOTEI010000000
VERSION     DAOTEI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_13228_cov_4.958020"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="P5313_09855"
     CDS             <1..169
                     /locus_tag="P5313_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HRY80708.1"
                     /translation="FFVSGPGSTTLPLYVYSMIRFGVSPVINSLSVVLIAGTVLITLL
                     ARNFLKYIAQK"
     gene            433..660
                     /locus_tag="P5313_09860"
     CDS             433..660
                     /locus_tag="P5313_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRY80709.1"
                     /translation="MAVTLYSTPSCSYCRLAKDWLRQRNVRFEEYNVAADPRRAEEMV
                     RKSGQMGVPVLDVNGRIILGFNTAEMERALK"
     gene            865..1659
                     /locus_tag="P5313_09865"
     CDS             865..1659
                     /locus_tag="P5313_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80710.1"
                     /translation="MNSDRVKEILLSVEEPKTEFSVIFSGKKSSLVNGLYKPQSREII
                     LHNRNFDDELQLIYTALHEYAHHLHCERKGFVYTGRAHTNEFWSIFHELLEKAEARGH
                     YRNVFEDSTEFKELTARIKTACVAENGRVMLEFGRLMIEAERLCREHKVRFEDYVDRV
                     LGLPRITATAAMKAASYQVPTEIGWDAMKLVSGIKDPDRRHAAQEAFQAGKSPETVKA
                     LIRAAPPGTDPEERLVKERERIERTIARLQDRLAQLDEELRRMEAG"
     gene            1768..2322
                     /locus_tag="P5313_09870"
     CDS             1768..2322
                     /locus_tag="P5313_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRY80711.1"
                     /translation="MDTEDTFRGDSYCGLYCGACEVLNLYRDSLATGKPASWEDLPGA
                     LKEVIDPASSIVCRGCKTDLVSPGCRDCVIRACAREKNVEACVLCPDSPCKLVSDRKA
                     YISENLEAILPHIKAKFPRAERIRAVGFETWRGEQARRWACPECGTPFTWYQKTCGRC
                     GRELEPLKEHNRRDEPAGSGRTVS"
     gene            complement(2319..3287)
                     /locus_tag="P5313_09875"
     CDS             complement(2319..3287)
                     /locus_tag="P5313_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HRY80712.1"
                     /translation="MDSIIQILVLTAFLAGGALCVKFRIAPPAKVTDILIKIALWALL
                     FVMGFRTGNDPEVASRIGQIGILGAAAAAAALAGTLAAILAGYAILGMFRRGAPEHPR
                     HGETARLGTGTGASGSSASQLRTSLLLLSVVAAGGLFGILLPPLTGIDLGTITGWVLN
                     ALLFFVGMQFAQSGLSFKAVFLRPDTLMIPVATAVGSILGGLALAPLFRLPAGHAMSL
                     TAGFGWYSLSGVLIANLGNPVLGSAAFLANLFRESLAFLTIPLLARTRWPHLAIGAGG
                     ATAMDVTLPLIEQCVGPESVPASFASGALLSLSVPVLVPFLFGLGS"
     gene            3465..4265
                     /locus_tag="P5313_09880"
     CDS             3465..4265
                     /locus_tag="P5313_09880"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRY80713.1"
                     /translation="MRRFDAEVLENRELSRDYRRIEVEWPGEAGTPAPGAFLTVRVSG
                     GTDPLLRRPFAFSGYDPGPGQASFIFQIRGPATRLLAGRRPGTALDVLGPLGKGFPDP
                     DPGGRPVLVGGGIGLGPILYAARDFGSRAARGGWEAPVLVLGFRTAAQVPDLDFPEGT
                     MICTDDGSAGFKGTAVQAAELAGAGGRYGPDAPPSYYACGPAPMMAALDRLAVVRYAP
                     FWAAAEQWMACGVGACMSCAVRLKDGSFARACAEGPVFDGASIDWGAM"
     gene            4262..5182
                     /locus_tag="P5313_09885"
     CDS             4262..5182
                     /locus_tag="P5313_09885"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRY80714.1"
                     /translation="MNPDLSVRIGNLVLPNPVGVASGTFGYGSEYESLMDVDALGALY
                     TKAVTPEPRPGNEVPRIVETPSGMLNSIGLANPGLDAFVRDKLPELRSRRCPVIVNVA
                     GASEDDYLRVLERLEDESGIAGYEINVSCPNVSHGGLAFGTDPALVERLTARLRGTLR
                     SGRPLIVKLSPNVTDIAEVARTAQAGGADAVSCVNTLVGMAVDVERLRPRLARITGGL
                     SGPAIRPVGVACVWRVSRAVTIPVIGIGGILEARDALEYLLAGASAVQVGTGNFLDPG
                     TAARVLAGIRDWMVRHGAARVPDIPGLLRA"
     gene            complement(5207..6442)
                     /locus_tag="P5313_09890"
     CDS             complement(5207..6442)
                     /locus_tag="P5313_09890"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY80715.1"
                     /translation="MNPQTQQSRLVLEAEATREYGTTVLKAARHRHFASRDVTATALA
                     DELGRRPEVEVLALLDEEGRPAGILTREGLFSLLGKPFGREVLGRTRAWELAESAPRM
                     DCHASLFAAGTGKAAADSPYRVLVDAEGRFRAILSSRDLAEHLSRITEEDIDLAGRIQ
                     ERLEAGNEALSNRLYGFEAWSRPAKGVGGDFWYSKRLEDGRAFFALCDVSGKGVAASL
                     VVSLVWGMLRMFDFRRGLYALLRSLNEALVATFHLEKYLTGFFGVYDPRTGVLEAADM
                     GHAHALVFREGRARRLDPGHRNLPVGVEPNLEPVLRRWRLRDGDALFVYSDGIPEQED
                     AEGGELGERRLAILVQKALRGGRALRDALPGALDLHRGTAPQQDDMSFILLTRPEGLR
                     SPMPRAEEPDTRVFLRPEA"
     gene            6575..6886
                     /locus_tag="P5313_09895"
     CDS             6575..6886
                     /locus_tag="P5313_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY80716.1"
                     /translation="MEWTLVSTDHDRGVEIRLTGDLDLYNAPRFSIYVLQKIEVGWSK
                     ILLDMRGVEYLDSTGAGSLIRIAQALRKKGAKLRCRNLGGSPRRVLEMANILPLLEET
                     R"
     gene            6893..7456
                     /locus_tag="P5313_09900"
     CDS             6893..7456
                     /locus_tag="P5313_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY80717.1"
                     /translation="MGRLIVRKLIVDERNELFDAQGMRRIEFPSEFERIRDYAAEVLR
                     DCPEAFREGNLLEQQLSEIIKNGIKHGNRCDPAKRLRVWFDFRKRVRFIVEDEGEGFA
                     DLEAWNEFYRKRQEALHAEDFDTFLAMASYRGPRSDDMDGGNSLVAALEYWNGGMIYN
                     GVRNKVGVVRWYTGGSDQPPAPRSDRS"
     gene            7513..8589
                     /locus_tag="P5313_09905"
     CDS             7513..8589
                     /locus_tag="P5313_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HRY80718.1"
                     /translation="MKFKEFLASFASFLSECPREEAGRSSRYVFLSDLHMGDGGTRDD
                     LAHNRELLEAILARHYLERGYTLVLNGDIEDLSKFRYSDIRTAWASLYEVFDAFAKAD
                     RLRKIVGNHDLGLLKIRDYPYGLIHGLVLEHGPDLLFAFHGHQGSPFFTNHDYISHFV
                     VRYLAKPLAIKNSSVDDHGKRRFRLERRIYRASKALGVASVIGHTHRPLFESRSKYDS
                     LRWAIEELVAEYPSATGGRRTEIADLVGLYRSELARMGRKELSRDLAGSRYYRERILV
                     PCLFNSGCATGRHGITALELADGALSLVQWTGAAGPKPYLRREALEAEPLEGTPNVRY
                     LLRRSDLDHVFARIRLLGRELPPA"
     gene            8711..9691
                     /locus_tag="P5313_09910"
     CDS             8711..9691
                     /locus_tag="P5313_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HRY80719.1"
                     /translation="MKKRIASIALAVVAVGAYAQGMFTWIDKVSDDKDKILPGVNPAA
                     VTGTIVTAGSSTVFPLSEAVAEQFKKDGFAGQITIDSIGSGAGFDRFTKSGETDISNA
                     SAKIKASHIEAAAKLSPPRVPIELRVGTDAIAVCVSTKNKFAKDVTMAELAKIFSTAV
                     YWSDVRPEWPKKEIKKFSPGTDSGTFEYFVEHVFKKDRKPLLEGKNLQLSEDDNILVR
                     GIGGDQYAIGYFGFAYYLENQKSLQVLALDGVAANQANVDAGKYALSRPLFLYSDAGI
                     INAKPQVAAFLTYYLTYVNDLIKKVGYFPAPAADLKASKQALAAAMKGRF"
     gene            9848..10780
                     /gene="pstC"
                     /locus_tag="P5313_09915"
     CDS             9848..10780
                     /gene="pstC"
                     /locus_tag="P5313_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRY80720.1"
                     /translation="MQHGHYREETTGVLSLARRSRPWEQAIQSFLFFCAAVSILTTLG
                     IVLTLGTESWTFFKHVSPFEFLFTFEWQPMIERFGIWPLFTATFMTSLAAMLIALPLG
                     LGVAIYLSEYAPNAVRETLKPILEVLAGIPTVVYGYFALTVVTPLLRNVFGQDRVDVY
                     NTLSAGLVMGVLILPLVSSMCEDALSSVPNSLREAAYAMGATKLELSLNVMVPSAFSG
                     IAAAFILGLSRAVGETMIVALAAGAGPNLTLNPFKAAETMTGHIVRISGGDIGYDTVD
                     YQSLFAIALVLFFVTLGLNVLSRRIIRKYGEVYE"
     gene            10777..12042
                     /gene="pstA"
                     /locus_tag="P5313_09920"
     CDS             10777..12042
                     /gene="pstA"
                     /locus_tag="P5313_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRY80721.1"
                     /translation="MSSETASGAAPTFRTDLTGRYRRGNVWAALFLTVTLLSVFFLAA
                     LIWTIVNDSFGYVILVNETDPESLTRDGRPLEELDAPSLAAVLEENLPGRRLRSLGNE
                     RPLAERSREDLYAIVMDEVVNPEVKATFDLSESLLAKEDIRAVLERDFPEGRLIFRAW
                     LNLKFLGAAQSSVAVAAGVRGAVLGTLMTILVTILVAFPVGIGAAIYLEEYAADNRLG
                     RFIRINIYNLAGVPSIIYGMLGLALFVRLAEPFTSGTAFGAAADPTTANGRTVLSAGL
                     TLALLILPVIIINAQEALRAVPGILRQSSLAVGATKWQTVWHHVLPASFDRILTGTVL
                     AVSRAIGETAPLVVVGASTFLSRDPTSVFSKFTTLPIQIYQWTARPQEEFRRIAAAAI
                     LVLLILMLSLNAVAILLRNRIRAKKRLSR"
     gene            12039..12860
                     /gene="pstB"
                     /locus_tag="P5313_09925"
     CDS             12039..12860
                     /gene="pstB"
                     /locus_tag="P5313_09925"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRY80722.1"
                     /translation="MKKNLNDSNRFEVAIAREAEGGGTSPYALEVRNLDIRYGDFPAV
                     KDVTMAFDKRQVTAIIGPSGCGKSTALRAFNRMNELIPSCSTDGEVLFHGINVYDPGV
                     DAVQVRRWIGMVFQKPNPFPKTIHENIAWGARINGYTGDMDELVESTLQQVALWDEVK
                     DKLKQSALALSGGQQQRLCIARAIAVKPEILLMDEPTSALDPISTAKIESLLDDLKKR
                     YTIIIVTHNMQQAGRVSDKTAFFLQGDLVEYAPTKDLFFNPKDERTENYISGRFG"
     gene            12860..>13228
                     /locus_tag="P5313_09930"
     CDS             12860..>13228
                     /locus_tag="P5313_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRY80723.1"
                     /translation="MNTRIQFMEELTRLHHDILAMGTRVEEDLRRALEALRTGNLDLV
                     QEVKAGDEVINALQIKIEDQTAVLIATQQPVARDLRELVTVFKVTDNLERIGDHAVHL
                     AKTARRLAEDPYGQSLERLER"
BASE COUNT         2374 a         4464 c         4228 g         2162 t
ORIGIN      
        1 cttcttcgtg tcgggcccgg gctccaccac cctgcccctc tacgtgtatt ccatgatccg
       61 gttcggggtc tccccggtga tcaactccct ctcggtggtg ctcatcgccg gcaccgtgct
      121 catcaccctt ctggcccgga atttcttgaa gtacatcgcg caaaaatgac ccgcccggga
      181 ttcaggtttc cggacttcac cacggagatc acggagggca cggagagaac ggcctttaaa
      241 ccatagagaa gacaggatga accagatgag gagaagctgg acaggatggg atgagggaga
      301 tggaatatcg agcatatccc gcgtccggac ccgatctcgg acatcctgtc catcctgtcg
      361 aaccccaaac ggccttgacg atacaccttc aatatattat ttttcatata taatcactcg
      421 taggaggccc ccatggctgt aactctatac tccaccccca gctgttccta ttgccgcctg
      481 gccaaggact ggctccggca gcggaacgtc cggttcgagg aatacaacgt ggccgcggat
      541 ccccgtcgtg cggaggagat ggtgcggaag tccggccaga tgggggtgcc cgtcctggac
      601 gtgaacggcc ggatcatcct gggcttcaac accgcggaga tggagcgggc cttgaaatag
      661 gcctttgaac cataatttcg acaggattaa caagatagga caagatttac aggatctgag
      721 atatagaaat cctccttact ttggtccaat cctgtttatc attcctttat cctgtccatc
      781 ctgtcggatc tatgaagtaa acccaccctt acgtcttgtt ccccgttcaa tgtacggcta
      841 gaatccgtgc ccgggggtgg ggagatgaac agcgaccggg tcaaggaaat cctcctgtcc
      901 gtggaagaac cgaagaccga gttctccgtg atcttttccg gcaagaagag ttccctggtt
      961 aacgggctgt acaagcccca aagccgggag atcatcctgc acaaccggaa cttcgacgac
     1021 gaacttcagc tgatctacac ggccctccac gaatatgccc accacctgca ctgcgagcgc
     1081 aagggcttcg tctacaccgg ccgggcccac accaacgagt tctggtccat cttccacgag
     1141 cttttggaaa aggcggaagc ccggggacac taccgcaacg tcttcgagga ctccacggaa
     1201 ttcaaggaat tgacggcaag gatcaagacc gcctgcgtgg ccgagaacgg ccgggtgatg
     1261 ctggagttcg gccggctcat gatcgaggcg gagcggctct gccgggaaca caaggtgcgt
     1321 ttcgaggact acgtcgaccg ggtcctgggc ctgccccgga tcaccgccac cgcggccatg
     1381 aaggccgctt cgtaccaggt cccgacggag atcggctggg acgccatgaa gctggtgtcg
     1441 gggatcaagg atccggaccg gcgtcacgcg gcccaggagg ccttccaggc cggcaagagt
     1501 cccgagaccg tgaaggccct gatccgggcg gccccgcccg gaacggaccc ggaggaacgc
     1561 ctggtaaagg agcgggagcg catcgagcga accatcgccc ggctccagga ccgcctggcc
     1621 cagctggacg aggagctccg gaggatggaa gcaggataga acccggcgga acccgcggtc
     1681 ccgggcaccg gagcctgccg tctgtacagg atggatcgga ggcctcatac tcaaacccgg
     1741 gcccggcgga cgggcacggg aggtaccgtg gataccgagg acacgtttcg aggcgactcc
     1801 tactgcgggc tctactgcgg ggcctgcgag gtgctcaacc tgtatcggga cagtctggct
     1861 accggaaaac ccgcaagctg ggaggacctc ccgggggcgc tgaaggaagt catcgacccc
     1921 gcttcctcca tcgtctgcag gggctgcaag acggatctgg tctctcccgg ctgccgggac
     1981 tgcgtcatcc gcgcctgcgc ccgtgaaaag aacgtcgaag cctgcgtcct ctgccccgat
     2041 tccccgtgta aactcgtctc ggatcgcaaa gcatacatct cggaaaacct ggaagcgata
     2101 ctgcctcaca tcaaggccaa attcccccgg gcggaacgga tccgggcggt cggcttcgag
     2161 acttggcggg gagaacaggc aagacgctgg gcctgtccgg agtgcggaac gcccttcacc
     2221 tggtaccaga agacctgcgg cagatgcggc cgggaactgg aacccctcaa ggagcacaac
     2281 cggcgggacg aacccgccgg atccggcagg accgtttcct aggagcccag tccgaacagg
     2341 aagggcacaa ggaccggaac cgagagggac agcagggccc cgctggcgaa gctggcgggc
     2401 acgctctcgg gccccacgca ctgctcgatc aggggcaggg tcacgtccat ggccgtggcc
     2461 cctccggctc cgatggccag atgcggccat cgggtacggg ccaggagggg tatagtaagg
     2521 aaggccaggg actcccggaa aaggttggcc aggaaggcag cggagcccag gacggggttg
     2581 cccaggttgg cgatcaggac ccccgacaga ctgtaccagc cgaaccccgc ggtcagggac
     2641 atggcgtgtc cggcgggcag gcggaagagg ggtgcaaggg ccaggccgcc caggatcgag
     2701 ccgaccgcgg tggcgacggg gatcatgagg gtgtcgggcc ggaggaagac ggccttgaag
     2761 gacagccccg actgggcgaa ctgcatgccc acgaagaaca gcagggcgtt caggacccat
     2821 ccggttatcg tccccaggtc gatccccgta aggggcggca ggaggatccc gaagagcccg
     2881 cccgcggcca cgacggacag caggagcaag gaagtccgaa gctgggaggc cgagcttccg
     2941 gaggcgcccg tgccggtacc aagccgggcg gtttccccgt gtcggggatg ttccggcgcc
     3001 cccctccgga acatccccag gatcgcatag ccggccagga tcgcggccag ggtccccgcc
     3061 agggcggccg cagccgcggc ggccccgagg atcccgatct gcccaatccg ggaagccacc
     3121 tcgggatcgt tgcccgtgcg gaaacccatg acgaagagca gggcccagag ggctatcttg
     3181 atgaggatat ccgtgacctt ggcgggcggc gctatccgaa atttgacgca gagggcgccc
     3241 ccggccagga aggccgtgag gacgagtatt tggatgatgc tgtccatggc ggcagtatag
     3301 cggtaaacag tgaatcgtga atagtaaata gtgaatggga atggaatacc taatcaattc
     3361 cgctgcagcc ttcggtggtg tctacaggtg ttatcattca cccattcacc cattcaccca
     3421 ttcacccatt cacccattca cccattcacg tctatactcc ccccatgcgc cgattcgacg
     3481 ccgaggttct ggaaaaccgc gagctctccc gggactaccg ccgcatcgag gtggaatggc
     3541 ccggggaggc ggggactccc gcgccggggg ccttcctgac cgtccgggtt tccggcggga
     3601 cggatcccct gcttcgccgc cccttcgcct tctcgggcta cgatcccggc cccggccagg
     3661 cttccttcat cttccagatc cggggtcccg ccacccggct cctggccggg cgcaggcccg
     3721 gcacggcctt ggacgtcctg ggtcccctgg gaaaggggtt tccggacccg gacccgggcg
     3781 gccggccggt gctcgtggga ggcggaatcg gcctggggcc cattctttac gcggcccggg
     3841 atttcgggag ccgcgcggcg cggggaggct gggaagcccc cgtcctcgtt ctgggtttcc
     3901 gtaccgcggc ccaggttccg gacctcgact tccccgaggg cacgatgatc tgtaccgacg
     3961 acggcagcgc cggattcaag ggcaccgcgg tccaggcggc ggagctggcc ggcgcgggcg
     4021 ggcggtacgg accggacgcc cctccctcct actacgcctg cggacccgcc cccatgatgg
     4081 ccgccctgga ccgcctggcc gtcgtccgat acgctccttt ctgggccgcg gcggagcagt
     4141 ggatggcctg cggcgtcggg gcttgcatga gctgcgcggt ccggctcaag gacggctcct
     4201 tcgcccgggc ctgcgcggag ggccccgtct tcgacggggc ctcgatcgac tggggggcga
     4261 tgtgaatccc gacctttccg tccggatcgg caacctggtc ctcccgaatc ccgtgggggt
     4321 ggccagcggg accttcgggt acggctcgga gtacgagtcc ctgatggacg tggacgccct
     4381 gggagccctg tacaccaagg cggtcacgcc ggagccgcgg cccgggaacg aggttccccg
     4441 gatcgtggag accccctcgg gtatgctcaa ctccataggc ctggccaacc cgggactgga
     4501 cgccttcgtt cgggacaagc tccctgagct ccggagccgg cgctgtccgg tcatcgtcaa
     4561 cgtggccggg gcctccgagg acgactacct ccgggtcctg gagcgcctgg aagacgagag
     4621 cgggatcgcg gggtacgaga tcaacgtgag ctgccccaac gtctcccacg gggggctcgc
     4681 cttcgggacg gatcccgccc tggtggagcg gctcacggcc cggctgcgcg ggacactccg
     4741 gagcggacgg cccctgatcg tgaagctctc cccgaacgtg acggacatcg ccgaggtcgc
     4801 ccggaccgcc caggccggcg gggcggacgc ggtgtcctgc gtcaacaccc tcgtcggcat
     4861 ggccgtcgac gtggagcgcc tccgtccccg gctggcccgg atcacggggg gcctgtccgg
     4921 tccggcgatc cggcccgtgg gagtggcctg cgtctggcgg gtctcccggg ccgtgacgat
     4981 accggtcatc gggatcgggg gtatcctgga ggcccgggac gccctggagt acctcctggc
     5041 cggagcttcc gcggtccagg tcggcacggg gaatttcctg gatcccggga ccgccgcccg
     5101 ggtactcgcg ggcatccggg actggatggt ccggcacggg gccgcccggg tcccggacat
     5161 cccggggctc cttcgggcct aggagcccga cgggcttgcg gacttttcac gcctccgggc
     5221 gcaaaaagac ccgagtgtcg ggctcctcgg cccggggcat cggggatcga agcccctccg
     5281 gccgggtgag caggatgaag gacatgtcat cctgctgggg agccgtcccc cggtgcaggt
     5341 cgagagcccc gggcagggcg tcccgtagtg cccggccgcc ccgaagcgcc ttctggacca
     5401 ggatcgccag gcggcgttcc cccagttccc ctccctccgc atcctcctgc tcggggatcc
     5461 cgtcgctgta gacgaagagg gcgtccccgt cccgcaggcg ccagcggcgc aggaccggct
     5521 cgagattcgg ctccaccccg acgggaaggt tgcggtgccc gggatccagc cgccgggccc
     5581 gtccttcccg gaagaccagg gcgtgggcgt gtcccatgtc cgcggcctcc aggactcccg
     5641 tccggggatc gtagaccccg aagaagccgg tgaggtactt ctccaggtgg aaggtggcca
     5701 ccagggcctc gttcagggac cgaaggaggg cgtacagccc cctccggaaa tcgaacatcc
     5761 tcagcatgcc ccagaccagg gatacgacca gggatgccgc gacccccttt ccggagacgt
     5821 cgcagagggc gaagaaggcc ctcccgtcct ccaaccgctt ggaataccag aaatccccgc
     5881 cgaccccctt ggccggccgg gaccaggcct cgaacccgta cagccggttc gagagagcct
     5941 cgttccccgc ctccaggcgc tcctggatcc tgcccgccag gtcgatatcc tcctcggtta
     6001 tccgggaaag gtgctccgcc aggtcccggg acgaaaggat cgcccggaac cgtccctccg
     6061 cgtccacgag tacgcggtac ggagaatccg ccgccgcttt cccggttcct gcggcgaaga
     6121 ggctcgcgtg gcagtccatg cggggcgcgg actcggccag ttcccaggct cgggtgcgac
     6181 ccaggacctc ccggccgaag ggcttgccca ggagggagaa cagtccttcc cgggtcagaa
     6241 tgccggcggg ccggccctcc tcgtccagga gtgcgaggac ttccacctcg ggccgtctgc
     6301 ccagctcgtc cgccagcgcc gtggcggtca cgtcccggga ggcgaaatgg cgatgccggg
     6361 cggccttgag aaccgtggta ccgtactccc gggtcgcctc ggcttccagc acgagccggg
     6421 attgctgtgt ctgcgggttc atgggcacca ctatggagat ttccggtcag cgaaggatga
     6481 ggaccccgtg aattctatgt tttctagccc ccggctaacg gggccctaac tcccggtccg
     6541 catccttcga ctcgatcgaa agccaggagg caccatggag tggacgctcg tctcgacgga
     6601 tcatgaccgc ggcgtggaaa tccgcctgac cggggatctg gacctgtaca acgcaccccg
     6661 gttcagcatc tacgtactgc agaagatcga ggtgggatgg tcgaagatcc tactggacat
     6721 gaggggagta gagtacctcg actcgaccgg ggcgggatcc ctcatccgga tcgcccaggc
     6781 gctgcggaag aagggggcga agcttcgctg ccgcaacctg ggcggctcgc cgcgacgggt
     6841 gctggagatg gcgaacatcc ttccgctcct ggaagagacc cggtaggcgc ccatgggacg
     6901 actgatcgta cggaaactga tcgtggacga gcgaaacgag ctcttcgacg cccagggcat
     6961 gcggcggatc gagttcccca gcgaattcga gagaatccgg gactacgcgg cggaagtgct
     7021 acgggactgc cccgaagcct tccgggaggg aaacctcctg gaacagcagc tgtcggagat
     7081 catcaagaac gggatcaagc acgggaatcg ctgcgatcct gccaagcggc tccgggtctg
     7141 gttcgatttc cggaagcggg tccgcttcat cgtcgaggac gagggggagg gattcgcgga
     7201 cttggaggcc tggaacgagt tctaccggaa gcggcaggag gccctgcatg ccgaggattt
     7261 cgacaccttc ctggcgatgg ccagctaccg gggcccccgg agcgacgaca tggacggagg
     7321 gaacagcctc gtcgcggccc tggagtactg gaacgggggc atgatataca acggcgtccg
     7381 gaacaaggtg ggcgtcgtac gctggtatac cgggggttcg gaccagccgc ctgcgccgag
     7441 gagcgaccgt tcctaaccgt ttcttgacga ttccctcacg ccggagtcat gaggatgagg
     7501 tttaatctca ccatgaagtt caaggaattc ctcgcgtcct tcgcctcgtt cctgtccgaa
     7561 tgcccccggg aggaagccgg ccggagctcc cggtacgtct tcctgagcga cttgcacatg
     7621 ggcgacgggg gtacccggga cgacctggcc cacaaccggg aactgttgga agcgatcctg
     7681 gcccggcact acctggagcg gggctacacc ctggtcctga acggggacat cgaggatctt
     7741 tcgaagttcc gatactccga catccgtacc gcctgggcct ctttgtacga ggtgttcgac
     7801 gccttcgcga aggcagaccg cctccggaaa atcgtgggca accatgacct gggactgctc
     7861 aagatccggg actatcccta cggactgatc cacgggctgg tcctggaaca cggccccgac
     7921 ctgcttttcg ccttccacgg ccaccaggga tcccccttct tcacgaacca cgactacatc
     7981 tcccatttcg tggtccggta cctagccaaa cccctggcca tcaagaactc cagcgtggac
     8041 gaccacggga agcggaggtt ccgcctggag cggaggatct accgggcgtc caaggccctc
     8101 ggcgtagcgt ccgtcatcgg gcacacccac cggcccctct tcgagtctcg gtcgaagtac
     8161 gacagcctgc gctgggccat cgaggagctg gtggcggagt acccttccgc gacggggggc
     8221 cggcggacgg agatcgccga cctcgtcggc ctgtaccggt ccgaactggc ccgcatgggc
     8281 cggaaggagc tctcccggga cctcgcgggc agccgctact accgggagcg catcctggtt
     8341 ccctgcctct tcaactccgg ctgcgccacg ggccgccacg gtatcaccgc cctggagctc
     8401 gcggacgggg ccctgtccct agtccagtgg acgggcgcgg cgggtcccaa accctacctg
     8461 cgtagggagg ccctggaagc ggagcccctg gaggggaccc cgaacgtccg atacctcctt
     8521 cgtcgatcgg acctggacca cgtcttcgcc cggatccggc ttctgggacg ggaactgccg
     8581 cccgcatgag ggctctcgat cctcctcgat ttaacgatct gctaacattt cccacgtcaa
     8641 ggcctaatct tgcgcgccta tggtagacgc gggttcgcaa ggacccgcag catcccacgg
     8701 gaggaaacgg atgaagaaga gaatcgcgag catcgcgctg gccgtcgttg ccgtcggcgc
     8761 ctacgcgcag ggcatgttca cctggatcga caaggtcagc gacgacaagg acaagatcct
     8821 gccgggcgtc aatcccgcgg ccgtcaccgg caccatcgtc accgccggct cgtccacggt
     8881 cttccccctc tccgaggccg tcgccgagca gttcaagaag gacggcttcg ccggccagat
     8941 caccatcgac tccatcggct ccggcgccgg gttcgaccgc ttcaccaagt ccggggaaac
     9001 ggacatctcc aacgcctccg caaagatcaa ggccagccac atcgaggccg ccgccaagct
     9061 ctccccgccc cgggttccga tcgagttacg ggtcggcacc gacgccatcg cggtctgcgt
     9121 ttccacgaag aacaagttcg ccaaggacgt taccatggcg gaactggcca agatcttctc
     9181 caccgccgtg tactggtccg acgtgcgccc cgaatggccc aagaaggaga tcaagaagtt
     9241 ctccccgggc acggactccg ggaccttcga gtacttcgtg gagcacgtgt tcaagaagga
     9301 ccgcaagccc ctcctggaag gcaagaacct ccagctctcc gaggacgaca acatcctggt
     9361 ccggggcatc ggcggcgacc agtacgccat cggctacttc ggcttcgcct actacctgga
     9421 gaaccagaag tccctgcagg tcctggccct ggacggcgtg gccgccaacc aggcgaacgt
     9481 ggacgccggc aagtacgccc tatcccgccc cctcttcctg tactccgacg ccgggatcat
     9541 caacgccaag ccccaggtgg ccgccttcct gacctactac ctgacctacg tgaacgacct
     9601 gatcaagaag gtcgggtact tccccgcccc cgccgcggat ctcaaggcct ccaagcaggc
     9661 cctggcggcc gcgatgaagg gccggttcta agcttggccg tgaacccggg cggaatgtcc
     9721 gcccggtcct tgcaacacct cactcgtccg gggccgcacg gccccggacg agtcatgacc
     9781 agagccgagg ccgtcccgga aggggtccgc ccttccgccg gccttgaaag ccagcgagat
     9841 gccacgcatg cagcacggac attatagaga ggagaccacc ggcgtccttt ccctggcgcg
     9901 gcggtcccgg ccctgggaac aggcgatcca gtcgttcctg tttttctgcg ccgccgtctc
     9961 gatcctcacc accctgggaa tcgtcctcac cctggggacc gagtcctgga ccttcttcaa
    10021 gcacgtcagc cccttcgagt tcctcttcac cttcgagtgg cagccgatga tcgagcggtt
    10081 cgggatctgg ccgctcttca cggccacttt catgaccagc ctggccgcca tgctcatcgc
    10141 cctgcccctg ggactggggg tggccatcta cctctccgag tacgccccga acgccgtgcg
    10201 ggagactttg aagccgatcc tggaagtcct cgcggggatt ccaaccgtcg tgtacggcta
    10261 cttcgccctg accgtggtga cgcccctcct gcgcaacgtc ttcggccagg accgggtgga
    10321 cgtctacaac accttgagcg cggggctcgt catgggggtg ctgatcctgc ccctggtgag
    10381 ctccatgtgt gaggacgccc tctcctcggt gccgaactcc ctgcgggaag cggcctacgc
    10441 catgggagcc acgaagctgg aactctccct gaacgtcatg gttccctccg cgttttccgg
    10501 gatcgccgcg gccttcatcc tgggcctgtc ccgggcagtc ggggagacca tgatcgtggc
    10561 cctggccgcg ggtgcgggac cgaacctcac cctgaacccc ttcaaggccg cggagaccat
    10621 gaccgggcac atagtccgga tctccggcgg ggacatcggg tacgacaccg tcgactacca
    10681 gagcctcttc gccatcgccc tggtcctgtt cttcgtgacc ctcggcctga acgtcctttc
    10741 gcgccggatc atccggaaat acggggaggt gtacgaatga gctccgaaac cgcatccggc
    10801 gccgccccga ccttccggac ggacctgacg ggaaggtacc gaagaggaaa cgtatgggcc
    10861 gcccttttcc tgacggtcac cctcctttcg gtgttcttcc tggccgccct gatctggacc
    10921 atcgtgaacg attccttcgg gtacgtgatc ctggtgaacg agacggatcc ggaatccctg
    10981 acccgggacg gcagacccct ggaggagctc gacgccccct ccctcgccgc ggtcctggag
    11041 gagaaccttc cgggtagacg gctccgctcc ctgggcaacg aacgccccct ggccgagcga
    11101 tcccgggagg acctgtacgc catcgtgatg gacgaggtcg tgaaccccga ggtgaaggcg
    11161 accttcgacc tctccgagtc gctcctggcc aaggaagaca tccgggcggt cctggagcgg
    11221 gatttcccgg agggccggct gatcttccgg gcctggctca atctcaagtt cctgggcgcc
    11281 gcccagtcca gcgtggccgt cgcggcgggg gtccgggggg ccgtcctggg caccttgatg
    11341 accatcctgg tgaccatcct ggtcgccttc cccgtcggca tcggcgcggc gatctatctg
    11401 gaggaatacg ccgcggacaa ccgcctgggc cgtttcatcc ggatcaacat ctacaacctg
    11461 gccggggttc cctccatcat ctacggcatg ctgggactgg ccctcttcgt gcgcctggcg
    11521 gagcccttca cctcggggac ggccttcggg gccgccgcgg accccaccac cgccaacggc
    11581 cggaccgtgc tgtccgcggg gctcaccctg gccctcctca tcctgcccgt gatcatcatc
    11641 aacgcccagg aagcccttcg ggccgtgccc gggatcctcc ggcaatccag cctggccgtg
    11701 ggcgccacga aatggcagac cgtgtggcac cacgtcctgc cggcctcctt cgaccggatc
    11761 ctgaccggga ccgtgctggc cgtgtcccgg gccatcggag agacggcccc cctggtggtg
    11821 gtgggcgcct ccaccttcct ctcccgggat cccacctccg tattctccaa gttcacgacg
    11881 ttgcccatcc agatctacca gtggaccgcc cgtccccagg aggagttccg gcgtatcgcc
    11941 gcggcggcca tcctggtcct tctgattctg atgctttccc tgaacgcggt cgcgatcctg
    12001 ctgcggaacc gaatccgagc caagaagagg ctgagccgat gaagaagaac ctgaacgatt
    12061 ccaaccgttt cgaggtggcc atcgcccggg aggcggaggg cggaggcacc tccccctacg
    12121 ccctggaggt ccggaacctg gacatccggt acggggattt cccggccgtg aaggacgtga
    12181 ccatggcctt cgacaagcgc caggtcaccg ccatcatcgg cccctcgggc tgcggaaaga
    12241 gcacggccct acgggccttc aaccgcatga acgagctcat cccgtcctgc tcgaccgacg
    12301 gggaggtgct cttccacggc atcaacgtct acgaccccgg ggtggacgcg gtccaggtcc
    12361 ggcgctggat cggcatggtc ttccagaagc ccaacccctt ccccaagacc atccacgaga
    12421 acatcgcctg gggagcccgg atcaacggct acaccgggga catggacgag ctggtggaat
    12481 ccacccttca acaggtcgcg ctctgggacg aggtcaagga caagcttaaa cagagcgccc
    12541 tggccctgtc cgggggccag cagcagcgcc tctgcatagc ccgggccata gccgtgaagc
    12601 ccgagatcct gctcatggac gagcccacca gcgccctgga tccgatctcc accgcaaaaa
    12661 tcgagagcct ccttgacgac ctcaagaagc ggtatacgat aatcatcgtg acccacaaca
    12721 tgcagcaggc gggccgggtc tcggacaaga cggccttctt cctccagggg gatctcgtgg
    12781 aatacgcgcc gaccaaggac ctcttcttca atcccaagga cgagcggacg gaaaactaca
    12841 tctccgggag gttcggctga tgaacacgcg catccagttc atggaggagt tgacccgcct
    12901 ccaccacgac atcctggcca tgggcacccg ggtggaggag gacctgcgcc gggccctgga
    12961 ggccctgcgg acgggaaacc tggatctcgt ccaggaggtc aaggccgggg acgaggtgat
    13021 caacgccctg cagatcaaga tcgaggacca gaccgccgtc ctcatcgcca cccagcagcc
    13081 cgtggcccgg gacctaaggg aacttgtcac ggtgttcaag gtgacggaca acctggaacg
    13141 gatcggggac cacgcggtcc acctggccaa gaccgcccgc cgcctggccg aggatcccta
    13201 cggccagtcc ctggagcggc tggagcgg
//
LOCUS       DAOTEI010000093        13199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3267_length_13199_cov_4.639379, whole genome shotgun sequence.
ACCESSION   DAOTEI010000093 DAOTEI010000000
VERSION     DAOTEI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13199
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3267_length_13199_cov_4.639379"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="P5313_09935"
     CDS             complement(<1..269)
                     /locus_tag="P5313_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80724.1"
                     /translation="MVPTFRRRGRPAVPFRSVSAALVLAAFASAAAAQDIRLFPGVPE
                     PPVVNARSVVVLEAARGRILYERDADIPIPPASLTKLMTLRVLYK"
     gene            complement(273..1664)
                     /gene="asnS"
                     /locus_tag="P5313_09940"
     CDS             complement(273..1664)
                     /gene="asnS"
                     /locus_tag="P5313_09940"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRY80725.1"
                     /translation="MKSIRDLLSSSPDGKTLTVKGWIRTKREAKNAVFLELNDGSCMA
                     NLQCVFDASGGFPESLRTELGRCGTGASAAVTGVLVPSPAAGQAVELKAEGLEVIGES
                     PAETYPLQKKNHSLEFLREIAHLRARTNTFGAVARMRNQMAFAIHTFFQERGFQWVHT
                     PLITASDCEGAGAMFQVTTLDLDALAKAGKPVDYSKDFFDKKAYLTVSGQLNVETYCM
                     ALSKVYTFGPTFRAENSNTTRHLAEFWMIEPELAFAELEDDIALAEDFLKHLFSWALD
                     KCAEDLAFFDKRVQAGIVDTLSAVVKSKFARMTYTEAVIELEKHATEFEFKPYWGCDL
                     QSEHEKYLTEKVVGGPVAVTDYPKEIKAFYMRLNPDGKTVRAMDVLVPRLGEIIGGSE
                     REYRHDVLLSRIRELGLPEEAYSWYMDLRRFGTVPHCGFGLGFERLLMYITGMQNIRD
                     VIPFPRAVHQADF"
     gene            1815..2330
                     /locus_tag="P5313_09945"
     CDS             1815..2330
                     /locus_tag="P5313_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HRY80726.1"
                     /translation="MYYSVPVAYLLWFIGGLGALGLHRFYLRKIGTGFVWFLTGGLAM
                     FGSVYDFLTLPRQVTEANIRAGMREALEMRAREPLRESMRQVGAGTAAGPRETIEKTI
                     LRTARKNGGYVTPGEVAIEGDVTVDQARTALDKLAGKGYAQMRVRTSGVIVYAFPEFF
                     QDGRTEFEEGI"
     gene            2416..2646
                     /locus_tag="P5313_09950"
     CDS             2416..2646
                     /locus_tag="P5313_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HRY80727.1"
                     /translation="MDNEDFRFEIVRPVGVIGEGGRGWRTEVNLVSWNGREPKIDLRA
                     WSPDHQKMGKGIGLTREEALRLVEILKAYLAE"
     gene            2648..3091
                     /locus_tag="P5313_09955"
     CDS             2648..3091
                     /locus_tag="P5313_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRY80728.1"
                     /translation="MSKDESVSVVAALAERGGRVFLARRAEHKGSPGLWELPGGKLEV
                     GEAPDAALARELREELGIEARVEKIAFDRSDLRIDSRAFRFLVFRASWDRDPASSTDH
                     DSWGYFGPRDIPFETLAPLDGTVLRRWAGEKAEAGDRGGLRERES"
     gene            3088..3477
                     /locus_tag="P5313_09960"
     CDS             3088..3477
                     /locus_tag="P5313_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80729.1"
                     /translation="MRTNTPPGIVRLASAILAVLAALAVSSCGARLPKSLPMDQNPVF
                     SGGIGWVVVSQAYARVKAEPRTDAPDVGHLRGGDILAVEGRERDPRSALSWYRIGMGG
                     TAGWLTGDQAVFFESRAAAERAAGRYR"
     gene            3474..5015
                     /locus_tag="P5313_09965"
     CDS             3474..5015
                     /locus_tag="P5313_09965"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HRY80730.1"
                     /translation="MIDRILRWLLPALVGGVIGYVTNWLAIKMLFRPLTEKKVFGIRI
                     PFTPGILPRERHALALSLGDVVSRDLLTEDVLTARFESEELRDSVTRAVRSLLDDALD
                     RRVSEVRSAASSAEPLLAEAAGRAYGAVSSSEAFRRAAASAVREGLGAARGLRLKDIL
                     SPGGRSLVSEWFRVPGRAEFTAEKAREAVRMRLEAAASEGKSLGDLLPLSNLARAAGA
                     AFDSAYPSFVSAARAFLARPDVRKELERYGGAVVRKALGRLSPLQRLFVGAAQYDKAV
                     LESLPATVADLAEAAESFLADERNRERFKSILLDRIQRSVEDPLASRPREHPLSPVSI
                     SDLAFRSVRALGERGAAGLGGTDLLGAGTLGDLLESLGPEAREGLFEAVGSWIAGFFA
                     SGSGGTTVGTFARAFADTLMDGLGDASLGAALGWDGEFRDRLARYLAEKGTAIAARES
                     RRILASIDLRRMVVEKVDALDMLVIEGIMLRVMSKEFKGITVLGGILGALIGALQPLL
                     ELLRP"
     gene            complement(5049..6383)
                     /locus_tag="P5313_09970"
     CDS             complement(5049..6383)
                     /locus_tag="P5313_09970"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRY80731.1"
                     /translation="MPSIRSVQDIYEYLLTFVNAEKGQAVEFKLDRMRDLARRLGDPQ
                     AVAPCLHVAGSKGKGSVSTMLARILEASGRKTGLYTSPHLLDFRERITRAGDFFPDSS
                     YLAAMRELKPLIAGAGPDRFAGNELPTFFELATLLAFLVYRGEGCDASVYEVGLGGRL
                     DSTNIVTPEASVITVIELEHTEYLGTTIPAIAGEKAGIIKPGVPAFTGSRDPEALEVF
                     RRTAGERGSALHILAEECSIRDVRLTRAGTEAVVAFRDREVFPEPVLIRTPLIGDAQA
                     ENAALAALTARLSSFRPSPEAVLEGIARASLPARFQILAGEPPVVLDGAHTPASMAYT
                     ADAFRRIFPGPAVLVFACAEDKRPAEMARVLAGQFRSVIVTRPGTFKKSEPETAAEAF
                     RAAGFEVDLVPDTEEALGIGMERARAAGIPLLVTGSFYLCAAALAAFRERTP"
     gene            complement(6414..8594)
                     /locus_tag="P5313_09975"
     CDS             complement(6414..8594)
                     /locus_tag="P5313_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY80732.1"
                     /translation="MRPRTLDEFVGQDHIVGPGRLLRRAIQKDQLASVIFSGPPGTGK
                     TTLARVIANTTASRFVTLNAVLDGVASVRAAVEEAAKNRDLYGRRTILFVDEVHRWNK
                     AQQDALLPWVENGTLILIGATTENPFFEVNRALVSRSRVFQLTALTQADLSEAARRAL
                     SDPERGYGRWKVSFEDGALEHLVDTADGDARSLLNALELAVETGGESWPPPEGSKIRV
                     TREAAQESIQRRAVLYDKDGDYHYDSISAFIKSLRGSDPDAALYWLARMIYAGEDPAF
                     LLRRMLISASEDVGLADPSALGVVEACARAFDRVGLPEGQFHLAQAALYLATAPKSNS
                     ALGYFDARGEIEKERAEVPSHLKDASRDGPGFGHGEGYLYPHAYRDHWVAQAYLPDTL
                     AGRIFYKPAETGYEGRIREEVLRRREAQVAAALDAAGSGIGPGASAGENLTYSEAADR
                     RAEWLSRSEARATERLAAIRDGIFARAAPRRHERTLVADAREGFLVWEALRRVPEGAV
                     CALVRTDRERILIEGYVAGLEEICRPSVVLHPRREPDVAAIADSGLPVFDLLVSRDLL
                     SPAPDKAALLAAWAAAFPAARIALAESPASEGGRLSRIPALKEAADKDLAARFEGFEE
                     GFYAGTDRPELSWKSADAEGWIRDAGLEKGRVETERGDYGRVFTQRELDSWLSPASAY
                     GRALASDFNEDEIRVLREALDRSVLGRPVPWPFTVAYITVPPRT"
     gene            complement(8659..9792)
                     /locus_tag="P5313_09980"
     CDS             complement(8659..9792)
                     /locus_tag="P5313_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80733.1"
                     /translation="MKRILNIAAAILLAAAVLVSCEPGPAGLFALLEEEEPLDKGTAA
                     FNANTAAFVTRVNGAPDYYYAAVGATLLRRDVAGTTWETVSVPGAPAGSIISSGVSFG
                     TDLYISYAPAADTIFHFDGTTWDATYDNLSTGDPVQSLLMASNGQMFAVTQASTGTSS
                     SDYSTEYSIFYDTAGTFASSGAAVTDVASGRPNSVATDGSDYWIVAGNKVFTGVENSL
                     AEDAIPADISATKPAFGAFYDGVRVLISGTGYLFNASAAYAKSGTFGSAERRLSSVIV
                     VPKNGGGNATLVGVKSWASDEYTGYYEYDAALNAGLFSSVVPSTAYDMVSDNSNYVTT
                     LDDLSIEGFYFDSVGQVLFARTVKGGLWSNTYSGGVWSGWDRE"
     gene            complement(9803..10420)
                     /locus_tag="P5313_09985"
     CDS             complement(9803..10420)
                     /locus_tag="P5313_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80734.1"
                     /translation="MRTPRFILAVLLTATLTTGAFAQTEPPASDLPAADFNANPYVRG
                     EQTLSFSLGTFFPITFVNLYAGGTADTNLKIGAGFGISYSYVVAPGFSVGGEIEGATC
                     GTTAESSFFMVPFTAKASWYFVKMPFEIVPSVSAGLAIVRYEGYTNFNPILKGGAAFL
                     WRQSSSWSFGLDAQAWTVWQFYGDIPQDNRLGIFLDTRLTAVYHL"
     gene            10649..11422
                     /locus_tag="P5313_09990"
     CDS             10649..11422
                     /locus_tag="P5313_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HRY80735.1"
                     /translation="MRRKVERLADTLVSAIKGWEGVEAVVLGEASEQEILDPYFALVV
                     DVYYKGDIPASQERCAAFGDPGAFETADSQNKDRFFLEELPVRVEYKSLDFLEDILRR
                     KYDLLWVFKGSGTYMFYRLERGKVLYDKSGWIEDVRRRIREFPDTFWQGLRATFQSKM
                     EHYLSDLGAAALREDGFFYTISLGGFMKYAAAALFMINHRFEPSHRSIAEQLRDLPLL
                     PEDFWGRWESLLRVDGEMSPSRKFQIAQLLTLSIFSLDR"
     gene            11419..13050
                     /locus_tag="P5313_09995"
     CDS             11419..13050
                     /locus_tag="P5313_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80736.1"
                     /translation="MIRPAIRASGALLLCALTASCAPIPPAPFALRGEDGAVHPLTPD
                     SRFPEGLEGTIGGDGRTSARYALKDPVRVRGARTALEIRYELAGAARLEIFPEGRGAP
                     PLVSSELPVGAGRIRWLLPLPAGTELSSFRFTALPAGPESKAAASTLRIEALALVPAF
                     FGFESLPEGARFSRGSSLARSASGSETVRIEHPFGPGEDPVIRIDSAARPLRIRAEGG
                     RSAVLDAGRPDGVFLPLSALGGSGAVTVEASGTEPIRAASVLPSRDLPGPLPLDLAVV
                     LALPPPADSRFPYQLYRWSAFPECLVFDFRDYAAQDTYLKRLAFFVEKEGFRGRLAPD
                     SEIARLHGWNAHDYRSEDLAAFFDKAGRTAFPLSPEEVELRGILEREGILVREGNRFK
                     PGTGVIISISRESTTYLRDLFLTHEAAHALFFLDDAYRNLARSLWTAQGPEERRFWNV
                     FLSNRDYDPSDAYLSYNELQAYLVQQIPSRLESWLKDVAYARLAKSYPDRAAAILADL
                     EAALPGFKAKAEALDAYLRKTYGFRGGSFRRLRFE"
BASE COUNT         2077 a         4340 c         4618 g         2164 t
ORIGIN      
        1 tccttgtaca ggacccgcag ggtcatgagc ttggtgaggc ttgcgggagg gatggggatg
       61 tccgcgtccc gctcgtacag gatccggccc cgggccgcct ccaggaccac cacggagcgg
      121 gcgtttacga cgggaggctc cgggactccc gggaacaatc gtatatcctg ggccgcggcc
      181 gccgacgcga aggcggccag gacaagagcc gcggagaccg aacggaacgg aacggccgga
      241 cgtccccggc ggcggaaggt cgggaccatg ccctagaagt ccgcctggtg cacggcccgg
      301 gggaagggga tcacgtcgcg gatgttctgc atgccggtga tgtacatgag gagccgctcg
      361 aatcccagac cgaacccgca gtggggcacg gtgccgaagc gccggaggtc catataccag
      421 gagtaggctt cctcgggaag cccgagttcc cggatccggg atagaagcac gtcgtggcga
      481 tactcgcgct cggacccgcc gatgatctcc cccagccggg ggaccaggac gtccatggcc
      541 cgtacggtct tcccgtcggg gttgaggcgc atgtagaagg ccttgatctc cttggggtag
      601 tccgtgaccg ccacggggcc gccgaccacc ttctcggtca ggtacttctc gtgctcgctc
      661 tggaggtcgc agccccagta gggcttgaac tcgaactcgg tggcgtgctt ctccagctct
      721 atcacggcct cggtgtaggt catccgggcg aacttggact tcaccaccgc cgaaagggtg
      781 tcgacgatgc ccgcctggac ccgcttgtcg aagaaggcca ggtcctcggc gcacttgtcc
      841 agggcccagg agaagaggtg cttaaggaag tcctccgcca gggcgatgtc gtcctccagc
      901 tccgcgaagg cgagttccgg ttcgatcatc cagaactccg ccaggtggcg ggtggtgttg
      961 gagttctccg cccggaaggt cggcccgaag gtgtagacct tgctcagggc catgcagtag
     1021 gtctccacgt tgagctggcc ggacacggtc aggtaggcct tcttgtcgaa gaagtccttg
     1081 gagtagtcca cgggcttgcc ggccttggcc agggcgtcca ggtccagggt ggtcacctgg
     1141 aacatggccc ccgccccctc gcagtcgctg gcggtgatga ggggggtgtg gacccactgg
     1201 aagccccgct cctggaagaa ggtgtggatg gcgaaggcca tctggttgcg catccgcgcc
     1261 acggcgccga aggtgttcgt ccgggcccgg aggtgggcta tctcccggag gaactccagg
     1321 gagtggtttt tcttctgaag gggataggtc tccgcggggg actccccgat gacctcgagg
     1381 ccctcggcct tgagctccac cgcctgcccc gccgcggggc tgggcacgag gacgcccgtg
     1441 acggccgcgg aggctccggt gccgcagcgg cccagttcgg tccggaggga ttcggggaag
     1501 cctccggagg cgtcgaagac gcactggagg ttggccatgc aggacccgtc gttgagctcc
     1561 aggaacacgg cgttcttggc ctcgcgcttg gtgcggatcc agcccttaac ggtcagggtc
     1621 ttgccgtcgg gggagctgga tagaaggtcc cggatactct tcatggcgta ctcctcggag
     1681 gtccgtgata gcatgatcgg agggggagag taaatactgg ggcggtggtc cgtggactgt
     1741 ggacagtggt ccactgttta ccgtccgccg tcctctgtgg tacattctcc ccattcctat
     1801 atcgggagct tgaaatgtac tacagcgttc ccgtcgcgta cctcctgtgg tttatcggcg
     1861 ggctcggcgc cctgggattg cacaggttct accttcgcaa gatcggcact ggcttcgtct
     1921 ggttcctgac cggcggcttg gcgatgttcg gctcggtgta cgatttcctg accctgcccc
     1981 ggcaggtcac cgaggcgaac atccgggcgg gcatgcggga agccctggag atgcgggccc
     2041 gggaacccct gcgggagagc atgcgccagg tgggcgcggg taccgccgcg gggccgcggg
     2101 agaccatcga gaagaccatc ctgcgcaccg cccgcaagaa cggcggctac gtgacccccg
     2161 gcgaggtggc catcgagggg gacgtcaccg tggaccaggc ccggacggcc ctggacaagc
     2221 tggccggcaa gggctacgcc cagatgcggg tccggaccag cggcgtcatc gtctacgcct
     2281 tcccggagtt cttccaggac gggaggaccg agttcgagga aggtatatag atcctccgtg
     2341 gggcttttct gaatcggccc tcgggaactg gcaagcgccc gatccggatt ccgccggtat
     2401 aggacggagg tacacatgga caacgaggat ttccgcttcg agatcgtccg acccgtggga
     2461 gtgatcggcg aaggcggtcg gggctggcgc accgaggtga acctggtttc ctggaacgga
     2521 cgggagccca agatcgacct gcgggcctgg agcccggacc atcagaagat gggaaaaggc
     2581 atcggcctga cccgggagga agccctccgg ctcgtcgaga tcctgaaggc ctacctggcg
     2641 gagtagcatg tcgaaggacg aatcggtttc cgtggtcgcg gccctggcgg agaggggcgg
     2701 gcgggtcttc ctggcccgcc gtgcggagca caagggttcc cccggacttt gggaactccc
     2761 cggtgggaag ctggaggtcg gggaagcccc cgacgcggcc ctggcccggg aactccggga
     2821 ggagttgggt atagaggccc gtgtggaaaa gattgcgttc gaccgcagcg acctgaggat
     2881 cgactcccgg gccttccggt tcctggtgtt ccgggcctcc tgggaccgcg accccgcatc
     2941 ctccacggat catgattcct ggggatactt cggtccccgg gatataccct tcgagaccct
     3001 cgcgcccctg gacgggacgg tccttcggcg ctgggccggg gagaaggctg aagccgggga
     3061 tcgcgggggg ctgcgggaga gggagtcatg agaacgaaca ctccacccgg aatcgtacga
     3121 ctcgcctcgg cgatcctggc cgtcctggcg gccttggccg tatcgtcctg cggggcccgt
     3181 ctgccgaaaa gccttcccat ggaccagaat cccgtcttct cgggcgggat cggctgggtc
     3241 gtggtgtccc aggcctacgc ccgggtcaag gcggaacccc ggacggacgc gcccgacgtc
     3301 gggcacctgc ggggagggga catcctggcc gtggaaggac gggagcggga tccccgctcg
     3361 gccctgtcct ggtacaggat cggcatgggc ggaaccgcgg gatggctcac cggggaccag
     3421 gccgtcttct tcgagagccg ggccgccgcg gaacgggccg ccggccggta ccgatgatcg
     3481 accggatcct gcgttggctc ctgcccgccc tggtgggcgg ggtcatcggc tacgtgacca
     3541 actggctggc catcaagatg ctcttccgtc ccctcacgga aaagaaggtg ttcggaatcc
     3601 ggatcccctt cacccccggc atcctgcccc gggaacggca cgccctggcc ctgagcctgg
     3661 gggacgtcgt ttcccgcgac cttctcaccg aggacgtcct gacagcccgg ttcgagtccg
     3721 aggaactgag ggacagcgtg acccgggcgg tccggtccct gctggacgat gccctggacc
     3781 gccgggtgtc cgaggttcgg tccgccgcgt cctccgcgga acccttgctg gcggaggcgg
     3841 cgggacgggc ctacggggcc gtctcctcct ccgaagcctt ccgccgggca gcggcttcgg
     3901 ccgtgcggga ggggctcggg gcggcgcggg gcctgcgtct gaaggacatc ctctccccgg
     3961 gcggccgatc cctggtttcg gaatggttcc gcgtccccgg ccgggcggag ttcacggcgg
     4021 agaaggcccg ggaggccgtc cggatgcggc tggaagccgc ggcctcggaa ggcaagtccc
     4081 tgggcgacct gctgcccctg tccaacctgg caagggccgc cggagcggcc ttcgattccg
     4141 cgtatccgag cttcgtatcc gccgcccggg ccttcctggc ccgtccggac gtccggaagg
     4201 agttggagcg ctacggcggg gcggtggtgc gcaaggctct gggccgcctg tcgcccctgc
     4261 agcggctgtt cgtgggcgcg gcccagtacg acaaggcggt cctggagtcc ctcccggcca
     4321 cggtggcgga cctggcggaa gccgcggaat ccttcttggc ggacgagcga aaccgggaac
     4381 gcttcaagtc catcctgctg gaccggatcc agcgtagtgt ggaggatccc ctggcttccc
     4441 gcccgcggga gcaccccctg tcccccgtga gcatctcgga tctggccttc cggtccgtac
     4501 gggccctggg ggagcgggga gccgcggggc tcggcgggac cgacctgctg ggagccggca
     4561 cgctggggga tctcctggag tccctgggac ccgaggctcg cgaaggcctg ttcgaggccg
     4621 taggatcctg gatcgcgggc ttctttgcct ccggaagcgg cgggacgacc gtcgggacct
     4681 tcgcccgcgc cttcgcggac acccttatgg acgggctcgg ggacgcgtcc ctgggcgcgg
     4741 ccctcggctg ggacggggag ttccgggacc ggctggcccg atacctggcg gaaaagggca
     4801 cggcgatcgc ggcccgggaa tcccggcgga tcctcgcgag catcgacctg cgcaggatgg
     4861 tggtcgagaa ggtcgacgcc ctggacatgc tggtgatcga gggcatcatg ctccgggtca
     4921 tgtccaagga attcaagggc atcacggtcc tgggcggcat cctgggcgcc ttgatcggcg
     4981 ccctccagcc cctcctggaa ctcctgcggc cctgagggct cggcggccca cagccggggt
     5041 gcccgccgct agggcgtccg ttcccggaac gcggccaggg ccgcggcaca gaggtagaag
     5101 gaccccgtga ccaaaagggg gatgccggcc gcccgggccc gttccatccc gatgcccagg
     5161 gcctcctccg tatcgggcac caggtccacc tcgaaccccg cggcccggaa ggcctcggcc
     5221 gccgtttccg gctcgctctt cttgaaggtt cccggccgtg tgacgatcac ggaccggaac
     5281 tgccccgcga ggacccgggc catctccgcc gggcgcttgt cctcggcgca ggcgaagacc
     5341 aggacggcgg gccccgggaa gatccgacgg aacgcgtccg ccgtgtaggc catgctcgcg
     5401 ggggtgtggg ccccgtccag gaccaccgga ggttccccgg ccaggatctg gaagcgggcg
     5461 ggcagggagg cccgggcgat cccctccagg accgcctcgg gggaaggccg gaacgaggag
     5521 agacgggccg tcagggctgc aagggccgcg ttctccgcct gggcatcccc gataaggggg
     5581 gtgcggatga gcacgggctc cgggaagacc tcccggtccc ggaacgcgac caccgcctcg
     5641 gttcccgccc gggtcaaccg gacatcccgg atcgagcact cctccgcgag gatatgtagg
     5701 gcggagcctc gttccccggc cgtcctgcgg aacacctcca gggcttcggg gtcccgggag
     5761 cccgtgaacg cgggcacacc gggctttatg atcccggcct tctcccccgc gatcgcgggg
     5821 atcgtggttc ccaggtactc ggtgtgctcc agttcgatca ccgtgatgac cgaggcctcg
     5881 ggggtcacga tgttcgtgga atccaggcgg cctcccaggc cgacctcgta gaccgaggcg
     5941 tcgcagcctt ccccgcggta aacgaggaag gccaggaggg tggccagctc gaagaaggtg
     6001 gggagttcgt ttccggcgaa ccggtcgggg cccgcccccg ctatgagggg tttcagttcc
     6061 cgcatcgcgg ccagatagga ggagtccggg aagaagtccc ccgcccgggt gatccgctcc
     6121 cggaaatcca ggagatgggg cgaggtatac aggcccgtct tccggcccga ggcctccagg
     6181 atgcgcgcca gcatcgtgga gaccgagcct tttcccttgg atccggccac atggaggcag
     6241 ggcgcgacgg cctggggatc ccccagtcgg cgggcaaggt cccgcatacg gtctagcttg
     6301 aactcgaccg cctggccctt ctcggcgttc acgaaggtca ggaggtactc gtagatatcc
     6361 tgcacggatc ggatgctggg catggctaat attcttgacc ccgggcgggc gtgtcaagtt
     6421 cggggcggaa ctgtgatata ggccacggtg aagggccagg gcacggggcg gcccaggacc
     6481 gagcggtcga gggcttcccg aaggacccgg atctcgtctt cgttgaaatc cgacgccaag
     6541 gcccggccgt aggccgaagc gggtgagagc caggaatcca gctcccgctg ggtgaatacg
     6601 cgtccgtaat ccccgcgctc cgtctcaacc cggccctttt cgagccccgc gtcccgtatc
     6661 cagccttcgg cgtccgcgga cttccaggac agctccggtc gatcggtgcc ggcgtagaac
     6721 ccttcctcga aaccctcgaa ccgcgccgcc agatccttgt cagcggcctc ctttaaagcc
     6781 ggaatcctgg aaagccggcc cccctcggaa gccggggact cggccagggc gatccgggcg
     6841 gccgggaagg ccgccgccca ggccgccagg agggcggcct tgtccggtgc gggggacaac
     6901 aggtcccggc ttaccaggag gtcgaacacg ggaagcccgg aatccgcgat tgcggcaaca
     6961 tcaggttcgc ggcggggatg gaggacgacg gagggtcggc agatctcctc caggcccgcg
     7021 acgtagccct cgatcaggat ccgctcccgg tccgtccgca ccagggcgca caccgccccc
     7081 tccggaaccc tgcgcagggc ctcccagacc aggaagcctt cccgggcgtc ggccaccagg
     7141 gtccgctcgt ggcgccgcgg ggccgcccgg gcgaagatcc cgtcccggat cgcggccagg
     7201 cgctccgtcg cccgggcttc ggaccgggac agccactcgg cccggcggtc cgcggcctcg
     7261 gagtaggtga ggttttcccc ggcggacgcg cccggtccga tcccggaccc ggcggcgtcc
     7321 agggccgccg ccacctgggc ctcccgtctg cggaggacct cctcccggat ccggccctcg
     7381 taccccgtct ctgcgggctt gtagaaaatt cgacccgcca gggtgtccgg aaggtaggcc
     7441 tgggccaccc agtggtcccg gtaggcgtgg ggatagaggt agccttcgcc gtggccgaag
     7501 cccgggccgt cccggctggc atccttgagg tgggagggga cctcggcccg ctccttctcg
     7561 atttcccccc gggcgtcgaa gtaccccagg gcgctgttgg acttgggggc tgtggccagg
     7621 tagagggcgg cttgggccag gtggaactgc ccctccggga ggcccacccg gtcgaaggcc
     7681 cgggcgcagg cttccacgac ccccagggca gagggatccg ccaggcccac atcctcggag
     7741 gcggaaatga gcatccggcg gaggaggaag gccgggtctt ccccggcgta gatcatccgg
     7801 gccagccagt agagggccgc gtcgggatcg ctcccccgga ggctcttgat gaaggccgag
     7861 atggagtcgt agtggtagtc cccgtccttg tcgtagagga ccgcacgccg ctggatgctc
     7921 tcctgggcgg cctcccgggt cacccggatc ttcgagccct ccgggggagg ccaggactcg
     7981 cccccggtct ccaccgccag ttccagggcg ttgaggaggc tccgggcgtc cccgtccgcg
     8041 gtgtccacca ggtgctccag ggccccgtcc tcgaaggaga ccttccagcg gccgtagccc
     8101 cgctccgggt cggaaagggc gcgccgggcc gcctcgctca agtccgcctg ggtcagggcg
     8161 gtaagctgga agacccggga ccgggagacc agggcccggt tcacctcgaa aaaaggattt
     8221 tcggtggtgg ccccgatcag gatgagggtg ccgttctcca cccagggcag aagggcgtcc
     8281 tgctgtgcct tgttccagcg gtggacctcg tccacgaaga ggatggtgcg gcggccgtag
     8341 aggtcccggt tcttggcggc ctcctccaca gcggcccgca ccgaagccac cccgtccagg
     8401 acggcgttca gggtgacgaa gcggctggcg gtggtgttgg cgatgacccg ggccagggtg
     8461 gtcttgcccg tgcccggggg acccgagaat atgacgctgg ccagctggtc cttctggatg
     8521 gccctgcgaa ggaggcggcc cggtcccacg atgtggtcct ggcccacgaa ctcgtccagg
     8581 gtccggggcc gcatccgggc ggccaggggt tcccgcccgc cccccgatcc cgcgaagagc
     8641 ggggagtcga aactcacctc actcgcggtc ccagccgctc cagaccccgc cgctgtaggt
     8701 attggaccag agaccgccct tcacggtccg ggcaaacagg acctgtccga cgctgtcgaa
     8761 gtagaagccc tcgatggaca ggtcgtccag ggtggtgacg tagttggaat tgtccgaaac
     8821 catgtcgtag gcggtgctgg ggacgacgct ggagaacagc cccgcgttga gcgcggcgtc
     8881 gtactcgtag taccccgtgt actcgtcgga ggcccaggac ttcacgccga ccagggtggc
     8941 gtttccgccg ccgttcttgg gcaccacgat taccgaggac aggcggcgct cggcgctgcc
     9001 gaaggtgccg gacttagcgt aggcggcgga tgcgttgaac agataaccgg ttccggaaat
     9061 cagaactctt accccgtcgt aaaacgctcc gaaggcgggc ttggttgcgc ttatgtccgc
     9121 gggaatcgca tcctcagcca gggagttctc gactcccgtg aacaccttgt tccctgcgac
     9181 gatccagtag tctgagccat ctgtggcgac agaattgggc cggccgctcg cgacatccgt
     9241 cacagccgcc ccggaggatg cgaaggttcc ggcggtgtcg tagaatatcg agtactccgt
     9301 ggaataatcg ctagacgagg tcccggtgga cgcctgggta accgcgaaca tctggccgtt
     9361 tgaggccatc aacagagatt ggaccggatc ccccgtggag aggttgtcgt aggtggcgtc
     9421 ccaggtggtg ccgtcgaagt ggaaaatagt gtccgccgcg ggtgcatacg agatataaag
     9481 atcggttccg aaagataccc cggaggagat gatggatccc gccggcgctc ccggcaccga
     9541 taccgtttcc cacgtcgtac ccgctacgtc cctgcgcagg agcgtagccc ctacggctgc
     9601 atagtaataa tccggggcgc cgttcaccct cgttacgaac gccgcagtgt tcgcgttgaa
     9661 ggccgcggtc cccttgtcca gcggctcctc ctcctccagc agggcgaaga gccccgcggg
     9721 cccgggctcg caggaaacca ggacggcggc ggccaggagg atggcggcgg cgatattcag
     9781 gattcttttc atggctcggc tcctagaggt ggtagaccgc ggtcaggcgg gtgtccagga
     9841 agatccccag gcggttgtcc tgggggatgt ccccgtagaa ctgccagacc gtccaggcct
     9901 gggcgtccag gccgaagctc caggaggagc tctggcgcca gaggaaggcg gccccgccct
     9961 tcaggatcgg gttgaagttc gtgtatccct cgtagcggac gatcgccagg cccgcggaaa
    10021 cggagggcac aatctcgaag ggcatcttca cgaagtacca ggaagccttg gcggtgaagg
    10081 gcaccatgaa gaacgaggat tccgcggtgg tgccgcaggt ggctccctcg atctccccgc
    10141 ccacggaaaa gcccggggcc accacatagc tgtaggagat gccgaagccc gccccgatct
    10201 tgaggttcgt gtccgcggtt cccccggcgt agaggtttac gaaggtgatc gggaagaagg
    10261 ttcccaggct gaaggagagg gtctgctcgc cccggacata gggattggcg ttgaagtccg
    10321 cagcgggcag gtccgaggcg ggaggctcgg tctgggcgaa ggcgcccgtc gtaagggtgg
    10381 ctgtcagcag taccgcgagg atgaatcgag gcgttcgcat tgttcctccg attgcgggat
    10441 agtatcacag gagccgttcc gtgaaaagcc gagggtacgt atcgaaaccc ctcgataaaa
    10501 cacccggccg ggcccggggg atacgcgggc cgcgaccgcg gcaggacgcc gggcctcgaa
    10561 tcccgaagcg ttccggccgc cggcgaggtt tccaaaggcg gggattcatg atagtatcga
    10621 ggtcaagaag cgagacaagg aagggaagat gcgccggaaa gtggagcgcc tggcggatac
    10681 tctggtttcc gcgatcaagg gatgggaagg tgtagaggcg gtggtcctgg gggaggcctc
    10741 ggagcaggag atcctggatc cgtacttcgc cctggtggtg gacgtctact acaagggaga
    10801 tatccccgca tcccaggagc gatgcgcggc cttcggggac cccggcgcct tcgagaccgc
    10861 ggattcccag aataaggacc ggttcttcct ggaggagctg cccgtccggg tggaatacaa
    10921 gagcctggat ttcctggagg atatcctccg gcggaagtac gaccttctgt gggtcttcaa
    10981 gggctccggc acgtacatgt tctaccgcct ggagcgcgga aaggtgctgt acgacaagtc
    11041 cggctggatc gaggacgtcc ggaggcggat ccgcgaattc ccggacacct tctggcaggg
    11101 gctccgggcg accttccagt ccaagatgga acactacctc tccgacctgg gtgcggcggc
    11161 cctccgggag gacggattct tctacaccat atccctgggc ggcttcatga agtacgcggc
    11221 ggccgccctg ttcatgatca accaccgctt cgagccctcc cacaggagca tcgcggaaca
    11281 gctccgcgac cttcccctgt tgccggagga cttctggggc cggtgggaat cccttctcag
    11341 ggtcgacggg gagatgtccc cctcccgcaa gttccagatc gcccagcttc tgaccctgag
    11401 catcttttcc ctggaccgat gatccggccc gcgatccggg cgtccggcgc cctcctgctg
    11461 tgcgccctga ccgcctcctg cgctccgatc cctcccgctc ccttcgccct gcggggcgag
    11521 gacggggccg tacacccctt gacgccggat tcccggtttc cggaaggact cgaggggacg
    11581 atcggcgggg acggccggac ctcggcccgg tacgccctga aggatccggt gcgggtccgg
    11641 ggagcccgca cggccctgga gatccgctac gagctggcgg gcgccgcccg actggagatc
    11701 tttccggagg gccggggcgc cccgcccctg gtgtcctcgg aactcccggt aggagcgggc
    11761 aggattcgct ggctccttcc ccttcccgcg ggcaccgagc tttcgtcctt ccggttcacg
    11821 gccctgccgg ccggaccgga atccaaggcc gcggcttcga cccttaggat cgaagctctc
    11881 gctctggtgc cggctttctt cgggttcgag agcctgcccg agggggcgcg gttctcccgg
    11941 ggttcgagcc ttgcgcggag cgcctcaggg tcggagaccg tccggatcga gcaccccttc
    12001 gggcccgggg aggatcccgt gatccggatc gactcggcgg cccggcccct tcggatccgg
    12061 gccgagggcg gccgatccgc ggtgctggac gccggccgcc cggacggcgt ctttctgccg
    12121 ctgagcgccc tgggaggatc cggggcagtg accgtggagg cctccggtac cgagccgatc
    12181 cgggcggcct ccgtcctgcc ctcccgggac cttcccggcc ccctgccgct ggacctggcc
    12241 gtcgtgctgg ccctgccgcc tcccgcggat tcccggtttc cctatcagct gtaccggtgg
    12301 agtgcctttc cggaatgcct ggtcttcgat ttccgggatt acgcggccca ggacacctat
    12361 ctcaagcgtc tggctttttt cgtggagaag gagggcttcc gcggacgcct ggccccggat
    12421 tcggagatcg cccgtctgca cggctggaac gcccacgatt accggtccga ggacctggcc
    12481 gccttcttcg acaaggccgg acgtaccgcc ttccccctct cccccgagga ggtcgagctc
    12541 cggggcatcc tggaacggga aggcatcctg gtacgggagg ggaaccggtt caaacccggc
    12601 acaggcgtga tcatctcgat atcccgggaa tcgacgacct atctaaggga cctgttcctc
    12661 acccatgagg ccgcccacgc cctcttcttc ctggacgacg cctaccggaa cctggcgcgt
    12721 tccctatgga cggcccaggg accggaggag cggaggttct ggaacgtctt cctgtccaac
    12781 cgggactacg acccctcgga cgcctatctc tcctacaacg agctccaggc ctacctggtc
    12841 cagcagatcc cctcccgtct cgaatcctgg ctgaaggacg tcgcctacgc ccggctggcc
    12901 aagtcctacc ccgaccgggc ggcggctatc ctggcggacc tggaggcggc ccttccgggc
    12961 ttcaaggcca aggcggaagc cctggacgcc tacctgcgga agacctacgg ctttcgggga
    13021 gggtccttcc ggaggctcag gttcgagtga ggttcaaagt ccggcctcca gctccgcgga
    13081 gacgatctcc agagcccaaa ctcgggtacg ttccatgagc tccggctgaa ggatccgctc
    13141 atcccccagc cctttgcgca gggctgcggc cgcgggcagg agggtcgtcc acaggtcct
//
LOCUS       DAOTEI010000094        13189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3274_length_13189_cov_4.548119, whole genome shotgun sequence.
ACCESSION   DAOTEI010000094 DAOTEI010000000
VERSION     DAOTEI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3274_length_13189_cov_4.548119"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="P5313_10000"
     CDS             complement(<1..275)
                     /locus_tag="P5313_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80737.1"
                     /translation="MKVKKYPSRAGMIGLAAILSLAASSCLSKEPDVPAGPGTAANQD
                     GRDLQPLSVTAPNGGTLVIKKAADPSNICFVPSEDLRTRLGDDAYDVV"
     gene            515..1672
                     /locus_tag="P5313_10005"
     CDS             515..1672
                     /locus_tag="P5313_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY80738.1"
                     /translation="MKRYAVILAVLVALALVGCGGKGPAKQDANVVKFGVFEPLTGAN
                     AAGGAEELDGIRLAQSLYPTVTVGGKEYKIELVIADNKSDKVEAANAGQRLVDSDKVN
                     VVLGSWGSGLSMAAGPIFKEKKIPAIGLSCTNPLVTKDNDFYFRVCFLDPFQGTVMAN
                     YAFKDVKAKKAVIIREVSNDYSVGLAKFFVDAFKQLTGDPNAILAELNYNTNDQDFSA
                     QLTQVKSLKPDVIFAPGNYTESALIIKQARELGITAPFLGGDTWEIAEFVNVGKQAVE
                     GAVMSAFFDNDAPLTPLSKTFVEEYQKKYGKLPSGTAVLGFDGYLLAIDAIKRADSID
                     PVKIRDAIAVTKGFAGAAGYVTLDANGDPIKSAVIKEVKDGKFVYKTTVNP"
     gene            1799..2692
                     /locus_tag="P5313_10010"
     CDS             1799..2692
                     /locus_tag="P5313_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY80739.1"
                     /translation="MELSTFFQQLANALSLGSLYALIAIGYTMVYGILRLINFAHGDV
                     FMLGPYFAFYAISMLLMPWWAAFVVAVGLTALFGIGLERVAYRPLRDSPKISVMISAI
                     GASFLIENLGIVIFGGRPKSFASIPLFDTVIKFGSVSILSVSLFIPVITFVSLFILLW
                     IVNRTKTGLAMRAVSTDLDAARLMAVDVNKTISFTFGIGSLLAGLGGIMWTLKYPQLN
                     PLMGVMPGLKCFIAAVIGGIGNISGAVLGGFLLGIIEIMIVAFLPELTGYRDAFAFVL
                     LIVVLLVKPAGLLGKAQTVKV"
     gene            2695..3765
                     /locus_tag="P5313_10015"
     CDS             2695..3765
                     /locus_tag="P5313_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY80740.1"
                     /translation="MEKKTLSRNLIRNLAGLAFLAVLIAAGDAFLGPFAVRILNLSAI
                     YIVLALSLNLVNGFTGLFSLGHAGFMAVGAYVSALLTMSPSLKEMNFFLAPIVPWLQG
                     VQLPFLLALLLAGTAAGFVGFLIGAPVLRLRDDYLAIATLGFSEIIRVLITNAQTLTN
                     GAQGLKGIPKYSTTWVVWGCAAATVLFMVLLLRSSYGRTLKAVRDDEVAAEAMGVNVA
                     RVKIVSFVASSFFAGVGGALLGHMITTIDPKMFILTLTFNILLIVVLGGNGSITGSVL
                     AAIIVTVLMEALRFLDMPMNFLFLKTNGLPGLRMVIFSILLMMVVLYRQRGLMGNKEF
                     SWDMLSSAGLLPRRRNGGKGAL"
     gene            3765..4520
                     /locus_tag="P5313_10020"
     CDS             3765..4520
                     /locus_tag="P5313_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80741.1"
                     /translation="MLRTESMTMRFGGLTAVADLNIEIEKNEIVGLIGPNGAGKTTAF
                     NMITGVYTPTEGRIRFRGRDVTGAPPHRITDLGIARTFQNIRLFREMNVLENVLVACH
                     LRMGVNFFTSVLHTPGYLRKNREAHEFAMSLLESVGLADAASESAVSLPYGKQRRLEI
                     VRALATKPALLLLDEPAAGMNPQESLELMDFIVHIRDKFDLTILLIEHHMQVVMGVCS
                     RMYVLDYGVTIAHGTPEEIQKNPKVIEAYLGVD"
     gene            4520..5242
                     /locus_tag="P5313_10025"
     CDS             4520..5242
                     /locus_tag="P5313_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80742.1"
                     /translation="MLKIDNLSVHYGGIHALQGINLEVADGKIVTLIGANGAGKSTTL
                     KAIVGLVKASSGSVSWNGETLSGRPVKDIVQKGVVLVPEGRRIFPNLTVDENLSLGAY
                     ARNDKAEIAADREKVFGLFPRLKERMKQKGGTLSGGEQQMLAVARALMTRPALLMMDE
                     PSLGLAPLIVKMIFDIVRQINKTGTTVLLVEQNAKAALEVADYAYVLETGRITLQGPG
                     KELLADDKVRSAYLGEAAQGRP"
     gene            complement(5414..6334)
                     /locus_tag="P5313_10030"
     CDS             complement(5414..6334)
                     /locus_tag="P5313_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80743.1"
                     /translation="MKAKLLVLVFAGCAFLAAAQQVVQVDWVEGTVERMVGSAWRAVD
                     IGDSFGTDTTLRLAKGASAELTSGGVHVRLLAPGTYKLADAFAKAKGGSPASDTAAKI
                     AKLAGKGDYNLVTATTAGVRGDLQATPPPSSIQWDEGKEDPSSTAAGSVESADPYSEV
                     IALYGQGKFPQAAKGARDLRLSAAPQAAFRSAYWEAASFFAQGLALPAIRTLEKTAPD
                     PAAPEYRASDFLAARIWMELSEWDKALAALGRYGTPDAPLEDRQVSLILSSVCYDSLK
                     KKADSRKALEAAVALDPASDLGKEAAARLK"
     gene            complement(6558..7442)
                     /locus_tag="P5313_10035"
     CDS             complement(6558..7442)
                     /locus_tag="P5313_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80744.1"
                     /translation="MSLSKFLQSGFMPLFVRLAPSALGRAVFQFLGKLYYNFANRKER
                     RLIRRNVRDLLRTAWNKEGGTVTGEVFEGIFDHYYEKLLLAAKPATYLEKFFRRRVAL
                     VHEDVIRRSQERGKGTLLVTAHWGAEELIPAILYLRGYPVTVILETSTERLRKILETQ
                     TAGRDVELLFASDPEGVLGKILDALARGRVLVTQCDEVDAWRRRKNRTIQLFGHTLFF
                     DHTLDFIAHRSGASVAAVFCRRFPGLRYRLICEEIAPDGAAAQDVAKRSLTLWERYTL
                     EAPEQWHQWKKWAEMKAI"
     gene            complement(7439..8695)
                     /locus_tag="P5313_10040"
     CDS             complement(7439..8695)
                     /locus_tag="P5313_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY80745.1"
                     /translation="MPAWYQDAAWRILRGIRGQSLLAGYNVSARFVDPEPGDSYVLLS
                     NHAHRLDPWVVGSLLSRTIRYMANLEGTNPAKAAFADLVGAYGKRKGAPDYAALKRTL
                     DLGRAGETLGIFPEGDRTWDGRPAPLRPGMGKLLRVLDKPVLLARQEGSYLTGPRWAQ
                     TERIGRWTVEFRVLSRERVAEEVPEALERGIRDFLAADDLRNPSLREVRFECSAPAAG
                     IGRLLWLCPSCGESDTVAGTGDRVSCRACGAEWTVDGNQRLEAGPGAGGGFADLRDWT
                     DFQRSRLAGRAAAAESLGIPAAGPGPVPEPRKAAESLPDPAGARSITASEGVELRRRT
                     PRGAEPFGRGRLELFRDALVFFPADGGVRAAFDPGRVLYFVDNFNNYCEYSYGSDRYR
                     LVFRGGNAYKWIEALEALRGGGGNPT"
     gene            9192..9488
                     /locus_tag="P5313_10045"
     CDS             9192..9488
                     /locus_tag="P5313_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY80746.1"
                     /translation="MASKIYVGNLSYSTSDDRLRQHFAQYGEVLSANIIVDRYSGQSK
                     GFGFVEMATPEAASAAIAALNGVALDGRQLRVNEANPREERPRREFRDRGDYNY"
     gene            9771..11183
                     /locus_tag="P5313_10050"
     CDS             9771..11183
                     /locus_tag="P5313_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY80747.1"
                     /translation="MKIVIVGAGMVGASIARELVEEKKDVILIEKDPEKARFASNALD
                     CLVLNEDGIDPETLKRAGTEDADWFISLMGTDEANIVACGVVSSAFPKPRTIARVRSS
                     YYGSLGAASKSFLGVDHVINPEIEAAQTIVQAAREGISGEILPLREARIRLRSIPAAE
                     DPRFPGHTIQEIRKAVGLDFLIPAVVRDGGLTLPAGDFEIQGDDLVYLLAEPKVLDVI
                     LGPAAEPQSQVRKVLIVGATTVGERVVEGLLRSGQETKRPWGKLFSALAGHSGPEITV
                     IDDNREHAKRFAHRFPSVTIVNRDLVEEDALEREGAGNADLVVCVTGAQSYNILTARL
                     AKELGARRAIALLLNNSFIKLAPKLELDALVSMRTVVGSSILNIIRKANIRTLHAFHE
                     DDVELVELSIQEESPAAGRKVRDLGLPKGALVAFVLHGTSSAIPTGDTLVQVGDTVGI
                     LTKKGLIERLEEVFGGRRGR"
     gene            11173..12627
                     /locus_tag="P5313_10055"
     CDS             11173..12627
                     /locus_tag="P5313_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRY80748.1"
                     /translation="MAGKPLLRILAILLGIVSAFMALCLAAGFLMGEGDRTLRAFAIP
                     VGIGAAGLALAWIGKVTRRRISLPPRDGFLFVALAWVFASALGALPFTLAGTMSYVDA
                     FFETISGFTTTGASILSDIEVIPKSLLLWRSTTHWLGGMGIVVLTVAILPLLGVGGLG
                     LMEAEAPGPSVDKITPRISSTAKILWLIYVALTVLEILLLMAGGMDWFDAVTHTFGTM
                     ATGGFSTKNLSVGHYRSGYIDVVITVFMLAAGLNFSLYFKLLSGKAISAFRDTEARTY
                     LGIFSVAALLIAWDLWRAGTFGGFGASLRYAGFQASSIITTTGFATADFAKWPPLSQT
                     ILFLLMFVGGCAGSTGGGIKVIRIVFLSKMAFTEMKYLLNPRGIYGIFVDGKSLRKNI
                     IYDTAAFVLLYIFLLLATTLVVSAGGFDILTSLTASLATVGNIGPGFGKVGPAFNYGF
                     FPDWIKLWLSFAMLAGRLEIYTIFILFTRAFWRR"
     gene            complement(12644..>13189)
                     /locus_tag="P5313_10060"
     CDS             complement(12644..>13189)
                     /locus_tag="P5313_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY80749.1"
                     /translation="LDQVEGGETVLSTVDEPNEFFGEMALIDGRPRSASAVAVKDTKL
                     LVVDRPAFEAMILSNGRFALKIIKVLSERIRSSNDLVSDLIETLPKERIQRGMADFAL
                     RTGERIHDGGIKAHLGDMKAWINGRMGIALDDLEAALFRMLKDGAIRWAATSAQTKEH
                     VILPEEFLSRVDRRAADRPAL"
BASE COUNT         2271 a         4433 c         4171 g         2314 t
ORIGIN      
        1 accacgtcgt aggcgtcgtc tcccaggcgg gtcctcaagt cctcggaggg cacgaagcag
       61 atgttcgagg gatccgcggc tttcttgatc acgagggttc ctccgttcgg ggccgtcacg
      121 gacaggggct ggaggtcccg gccgtcctgg ttcgccgcag ttccgggtcc cgccggaacg
      181 tcgggttcct tggaaaggca ggacgaggcg gccagggaca ggatagccgc aagacctatc
      241 atccccgctc gggatggata tttcttgact ttcatactaa ggaatatact tcgccggacg
      301 tcctggatca atgaattcgc ggagtccggc ggtttcggcc gattcgcggt gtccgccgga
      361 cccgatgtgg ataccgcggc cccaggctcc cgaaggatag aatttcgaga atagaaatac
      421 cgtttctatt aagaaaaacg atttgacttt tatcgattcc ggggctacga tggtccatcc
      481 aacttcgcaa gaaatcgctc acaaggaggc atctatgaag cgatatgcgg tcatcctcgc
      541 ggtcctcgtc gccctggccc tggtcgggtg cggcggcaaa ggtcccgcca agcaggacgc
      601 caacgtagtc aagttcggag tcttcgagcc cctcaccggc gccaacgccg cgggcggcgc
      661 cgaggaactg gacggcatcc gcctggccca gagcctgtat cccaccgtca ccgtcggcgg
      721 caaggaatac aagatcgagc tggtcatcgc ggacaacaag tccgacaagg tggaggccgc
      781 caacgccggc cagcgcctgg tggacagcga caaggtgaac gtggtcctgg gatcctgggg
      841 ctcgggcctc tccatggccg ccggcccgat cttcaaggag aagaagatcc ccgccatcgg
      901 gctttcctgc accaaccccc tggtgaccaa ggacaacgac ttctacttcc gcgtctgctt
      961 cctggatccc ttccagggca ccgtcatggc caactacgcg ttcaaggacg tcaaggccaa
     1021 gaaggccgtc atcatccgcg aggtgtccaa cgactactcc gtgggcctgg ccaagttctt
     1081 cgtggacgcc ttcaagcagc tgaccgggga tcccaacgcc atcctcgccg agctcaacta
     1141 caacacgaac gaccaggact tctccgccca gctgacccag gtcaagagcc tgaagccgga
     1201 cgtcattttc gcccccggca actacaccga gagcgccctg atcatcaagc aggcccgtga
     1261 gctcgggatc acggccccct tcctgggcgg cgacacctgg gaaatcgcgg agttcgtgaa
     1321 cgtgggcaag caggccgtgg aaggcgcggt catgtccgcc ttcttcgaca acgacgcccc
     1381 cctgaccccg ctttccaaga ccttcgtgga ggagtaccag aagaagtacg gcaagctgcc
     1441 ctcgggcacc gcggtgctgg gcttcgacgg ctacctcctg gccatcgacg cgatcaagcg
     1501 cgccgactcc atcgacccgg tcaagatccg ggacgccatc gccgtgacca agggcttcgc
     1561 gggcgccgcg ggctacgtca ccctggacgc caacggggac ccgattaaga gcgcggtgat
     1621 caaggaagtc aaggacggca agttcgtcta caagaccacg gtcaatccgt aagcgaatcc
     1681 gcacggggag ccggcgccgg gcatgtcccg gcggccggcc ctgttcagcc cgcgctacgg
     1741 gaccgacatc cggccgccgg cgcgggcatt ttattccgtc cgacaaagga tccgtaccat
     1801 ggagttgtcc accttcttcc agcagctcgc caacgcgctc agcctgggaa gcctgtacgc
     1861 cctgatagcg atcggctaca cgatggtcta cggcatcctc aggctcatca acttcgccca
     1921 cggcgacgtc ttcatgctgg gcccctactt cgcgttctac gcgatctcga tgctcctgat
     1981 gccctggtgg gccgcgttcg tcgtggccgt gggcctcacg gccctcttcg gcatcggcct
     2041 ggagcgggtg gcctaccgcc ccctccggga ctcccccaag atctccgtga tgatcagcgc
     2101 tatcggcgcc tccttcctga tcgagaacct ggggatcgtg atcttcggag gccgtcccaa
     2161 gagcttcgcc agcattcccc tcttcgacac ggtgatcaag ttcggctccg tatccatcct
     2221 ctcggtcagc ctcttcatcc cggtcatcac cttcgtcagc ctcttcatcc tgctgtggat
     2281 cgtgaaccgc accaagaccg ggctggccat gcgggcggta tccacggacc tggacgcggc
     2341 ccggctcatg gccgtggacg tcaacaagac catctccttc accttcggca tcgggtccct
     2401 cctggcgggg ctgggcggga tcatgtggac cctgaagtac ccccagctga accccctgat
     2461 gggcgtgatg ccgggcctga agtgcttcat agccgcggtc atcggaggga tcgggaacat
     2521 ctccggcgcc gtgctgggcg gattcctcct ggggatcatc gagatcatga tcgtggcctt
     2581 cctgcccgaa ctgaccggtt accgggacgc cttcgccttc gtgctcctga tcgtggtgct
     2641 cctggtcaaa cccgccggcc ttttgggcaa ggcccagacg gtgaaggtgt agccatggaa
     2701 aagaaaaccc tttcccgaaa cctgatccga aacctcgcgg ggctcgcctt cctggccgtc
     2761 ctgatcgccg ccggggacgc cttcctgggc cccttcgcgg tacggatcct caacctctcg
     2821 gccatctaca tcgtcctggc cctctccctg aacctggtga acgggttcac ggggctcttc
     2881 tccctgggac acgcgggctt catggccgtg ggcgcctacg tcagcgccct gctcaccatg
     2941 tcgccgtccc tcaaggagat gaacttcttc ctggcgccca tcgtgccctg gctccaggga
     3001 gtccagcttc ccttcctcct ggccctcctg ctcgcgggta ccgccgcggg cttcgtgggc
     3061 ttcctgatcg gggccccggt actgaggctc cgggacgact acctggccat cgccaccctg
     3121 ggcttttccg agatcatccg ggtcctcatc acgaacgccc agaccctcac gaacggagcc
     3181 cagggactca agggcatccc caagtactcc accacctggg tggtctgggg ctgcgccgcc
     3241 gctacggtct tgttcatggt cctcctgctc cggtccagct acggccggac cctgaaggcc
     3301 gtccgggacg acgaggtggc cgcggaggcc atgggcgtga acgtcgcccg ggtcaagatc
     3361 gtctccttcg tggcctccag cttcttcgcc ggggtcgggg gggccctcct gggccacatg
     3421 atcacgacca tcgaccccaa gatgttcatc ctgaccctga ccttcaacat ccttttgatc
     3481 gtggtcctgg ggggcaacgg ctccatcacg ggctccgtgc tggcggccat catcgtgacc
     3541 gtgctgatgg aggccctgcg cttcctggac atgcccatga acttcctgtt cctcaagacc
     3601 aacggcctgc cgggcctccg gatggtgatc ttctcgatcc tgctcatgat ggtggtcctc
     3661 taccgccaac ggggcctcat ggggaacaag gagttctcct gggacatgct gtcctccgcg
     3721 ggcctcctgc cgcggcgcag gaacggcgga aagggggccc tctaatgctg cgcaccgaga
     3781 gcatgaccat gcgcttcggg ggactcaccg ccgtggcgga cctcaacatc gagatcgaga
     3841 agaacgagat cgtgggcctg atcggcccga acggggccgg caagaccacg gccttcaaca
     3901 tgatcaccgg ggtctacacc cccacggagg gccgcatccg gttccggggc cgggacgtga
     3961 ccggcgcgcc cccccacagg atcacggacc tgggcatagc ccggaccttc cagaacatcc
     4021 ggctgttccg ggagatgaac gtcctggaaa acgtcctcgt ggcctgccac ctccgcatgg
     4081 gggtgaactt tttcacctcc gtactccaca ccccgggcta cctccggaag aaccgggaag
     4141 cccacgagtt cgcgatgtcc ctgctcgagt ccgtgggcct ggccgacgcg gcctccgagt
     4201 ccgcggtctc cctgccctac ggaaagcaga ggcggctgga gatcgtccgg gccctggcca
     4261 caaagccggc cctgctcctg ctggacgagc ccgcggcggg catgaacccc caggagtccc
     4321 tggagctcat ggacttcatc gtccatatcc gcgacaagtt cgacctgacc atcctgctga
     4381 tcgagcacca catgcaggtg gtcatggggg tctgctcccg gatgtacgtc ctggactacg
     4441 gggtcaccat agcccacggg acgccggagg aaatccagaa gaatccgaag gtcatcgaag
     4501 cctacctggg ggtggactga tgctcaagat cgacaacctc tccgtgcatt acggaggcat
     4561 ccacgccctg cagggcatca acctcgaggt ggcggacggt aagatcgtca ccctgatcgg
     4621 ggccaacgga gcagggaaga gcacgaccct caaggccatc gtgggcctgg tgaaggcctc
     4681 ctcgggttcc gtctcctgga acggggaaac cctctcgggc aggcccgtga aggacatcgt
     4741 gcagaaaggc gtggtcctgg tgcccgaggg gcgccggatc ttccccaacc tgacggtgga
     4801 cgagaacctc tccctggggg cctacgcccg gaacgacaag gccgagatcg cggcggaccg
     4861 ggagaaggtc ttcggactct tcccccggct caaggagcgg atgaagcaga agggcggaac
     4921 cctgtccggc ggcgagcagc agatgctggc cgtggcccgg gccctcatga cccggccggc
     4981 cctgctcatg atggacgaac cctccctggg gctggccccc ctgatcgtca agatgatctt
     5041 cgacatcgtg cggcagatca acaagacggg gaccacggtc ctcctggtgg agcagaacgc
     5101 caaggcggcc ctggaggtgg cggactacgc ctacgtcctg gagaccggcc gcatcaccct
     5161 gcaggggccg ggaaaagagc tgctggcgga cgacaaggta cggtccgcgt atttagggga
     5221 agccgcgcaa ggacggccgt agcggccgtc cttgcggcga cgagctggcg ttcgtcgaat
     5281 atacgcagga gccacatctc tactgtcgaa atcgggtttt caagatggtc gacgaagccc
     5341 gcggggcttc gttgccataa aaaaggacgg ccgaggccgt ccttttttat gctctccgtg
     5401 gtgaatcctg gaatcacttc agccgggcgg cggcttcctt ccccaggtcg ctggccggat
     5461 ccagggccac ggcggcctcc agggccttcc gggaatcggc cttcttcttc aaggagtcgt
     5521 agcacaccga cgagaggatc agggacacct ggcgatcctc caggggggcg tccggggttc
     5581 cgtagcgccc gagggccgcc agggccttgt cccactcgga cagctccatc cagatgcggg
     5641 cggccaggaa gtccgaggcc cggtattccg gcgcggccgg atccggtgcg gtcttttcca
     5701 gggtccggat ggcgggcagg gccagcccct gggcgaagaa ggaggcggct tcccagtagg
     5761 cggaccggaa ggcggcctgg ggggcggcgg agagacggag atcccgggcg cccttggcgg
     5821 cctgcgggaa tttcccctgg ccgtacaggg cgatcacctc agaatagggg tccgcggact
     5881 ccacggagcc cgccgccgtc gaggatggat cctccttgcc ctcgtcccac tgaatgctgg
     5941 aaggcggagg tgtagcctga agatctcccc gtacgccggc cgtggtggcc gtgaccaggt
     6001 tgtagtcgcc cttcccggcc agcttggcga tcttggccgc cgtatccgag gccggggaac
     6061 cgcccttggc cttggcgaag gcgtcggcga gcttgtaggt gcccggggcc aggaggcgga
     6121 catggacgcc gccggaagtc agttccgcgg aggcgccctt ggccagccgg agggtggtgt
     6181 ccgtgccgaa ggagtcgccg atgtcgacgg cgcgccaggc ggacccgacc atgcgctcca
     6241 cggttccctc cacccagtcc acctgcacta cctgctgggc ggcggccagg aaggcgcatc
     6301 ccgcgaacac caatacgagc aacttcgctt tcatggatag aagtatatcg cgaatccgcg
     6361 gaaccgcaaa gaatttgacc cgatgacctt ttcgggattc aaccacggag gtcacggagg
     6421 atccgatcct gggcagccgg tttgccggat cctatccgcc ttggaaaaac tttgttctgg
     6481 gcagccgctt tctcggatcc cacggggatc cgagaaagcg aatatttagc gatcgagaag
     6541 cgaacgcttt tcgatcgcta aatggctttc atttccgccc atttcttcca ctggtgccac
     6601 tgctccgggg cctccagggt gtagcgctcc cagagggtaa gggatcgttt ggccacgtcc
     6661 tgggccgcgg ctccgtccgg tgcgatctcc tcgcagatca ggcggtaccg cagtcccggg
     6721 aagcggcgac agaagaccgc cgccacggac gcacccgagc ggtgggcgat gaagtctagg
     6781 gtgtggtcga agaagagggt atgtccgaag agctggatgg ttcggttttt ccggcggcgc
     6841 caggcgtcca cctcgtcgca ctgggtgacc aggacccggc cccgggccag ggcgtccagg
     6901 atcttaccga gaaccccctc ggggtccgag gcgaaaagga gctccacgtc ccggcccgcg
     6961 gtctgggtct ccaggatctt ccgcagccgt tccgtggaag tctccaggat cacggtcacg
     7021 ggatagcccc ggaggtagag gatggcgggg atgagttcct ccgctcccca gtgggccgtg
     7081 accaggaggg tgcccttccc ccgctcctgg gatctccgga tcacatcctc atggaccagg
     7141 gcgacccgtc ggcgaaagaa cttctccaga tacgtcgcgg gcttggccgc caggaggagc
     7201 ttctcgtagt agtggtcgaa aatgccctcg aaaacctcgc cggtcacggt gccgccctcc
     7261 ttgttccacg cggtccggag gaggtcccgg acgttgcggc ggatgaggcg gcgctccttg
     7321 cgattggcga agttgtagta cagcttgccc aggaactgga acaccgcccg cccgagcgcg
     7381 gagggcgcca ggcgcacgaa caggggcatg aacccgcttt gcaggaactt ggaaaggctc
     7441 atgtcgggtt tcctccgccg ccccggagcg cctccagggc ctcgatccat ttataggcgt
     7501 tcccgccccg gaagaccagc cggtaccggt cgcttccgta ggaatattcg cagtaattat
     7561 tgaaattgtc cacaaaatag agcacccggc cggggtcgaa ggccgcccgg acgccgccgt
     7621 ccgccgggaa aaagaccagg gcgtcccgga agagctcgag ccggccccgg ccgaagggtt
     7681 ccgcgccccg gggggtcctg cgccgcagtt ccaccccctc ggaggccgtg atcgaccgcg
     7741 ctccggcagg gtcgggcagg gattcggcgg cctttcgggg ctccggcacg ggaccgggcc
     7801 ccgccgcagg aatcccgagg gattcggccg cggccgcccg cccggcaagc cgggaccgct
     7861 ggaagtccgt ccagtcccgg aggtccgcga atcccccccc ggcgcccggc ccggcttcca
     7921 agcgctggtt tccgtccacg gtccactccg caccgcaggc ccggcaggag acgcggtctc
     7981 cggttcccgc gacggtgtcc gactccccgc aggagggaca gagccacaga aggcggccga
     8041 tcccggccgc cggggcggaa cactcgaacc ggacctcccg gagcgaggga ttccggaggt
     8101 cgtctgcggc caggaagtcc cggatccctc gttccagggc ctccggaacc tcctccgcga
     8161 ccctttcccg ggaaaggacg cggaattcca cggtccagcg gccaattctc tcggtctggg
     8221 cccatcgggg accggtcagg taggaccctt cctggcgggc caggagcacg ggcttgtcca
     8281 ggacccggag gagcttcccc atccccggcc gcaggggtgc cggccggccg tcccaggtcc
     8341 ggtcaccctc ggggaagatg cccagggttt ccccggctcg ccccagatcc agggtccgct
     8401 tgagggcggc gtagtccggg gcccccttgc gcttgccgta ggccccgacc aggtccgcga
     8461 aggcggcctt ggccgggttg gttccctcga ggttggccat ataccggatg gtgcgggata
     8521 ggagggatcc gacgacccag ggatccagac gatgggcgtg gttcgaaagg agaacgtagg
     8581 aatcccccgg ttcggggtcg acaaaacggg cggagacgtt gtacccggcc aggaggctct
     8641 gtccccggat tccccggagg atcctccaag ccgcgtcctg gtaccatgcg ggcatcggcc
     8701 cccctagaat tcgccgcggc ggatatccgt tcgtgtccga tccgcacgcg gcagtttccc
     8761 acgggcggat cagggcgggg tgagagtatc atgagtgatt gaataaaaaa agcggggaaa
     8821 ccgcggacct ccgcgatcgg tccttgacga gcttttaaaa ccggtgtatg gttcaccgtg
     8881 gtatacggcg ccgtcgcgcg atcgtatacc aggagaacga tcttattctc tgcacgaatt
     8941 cgtacgaaga gtagcttgaa cggaaactga actgttggat ttaaggaacg attcgaccgc
     9001 ttcgcgcgcg caatctcgca agccacccct gcgccgctcc gctcgccctt ccctttcccc
     9061 gacgtcgatt ggccgctctc ccctcttcca agaagcgtat gcaaagaaaa gatcctcgaa
     9121 cgtccggctt cgatcggacg gaatttcacg agtgtccccg gccgcagagc gggggaccgc
     9181 aaggactaca catggctagc aagatctacg tcggcaacct cagctacagc accagcgatg
     9241 accgcctccg ccagcacttc gcccagtacg gcgaggtcct gtccgcgaac atcatcgtgg
     9301 accgctatag cggccagtcc aagggcttcg gcttcgtgga gatggctacc ccggaagccg
     9361 cctccgccgc catcgccgcc ctgaacggcg tcgccctgga cggtcgccag ctccgcgtca
     9421 acgaggccaa cccccgcgag gagcgccccc ggcgcgagtt ccgcgaccgc ggcgactaca
     9481 actactaaaa accgcgcggt tcgccccttc cgggctcccg gagggggtga agacccggcc
     9541 cgagggccgg gtcttccttt ttccgcccgg gttcggcatt gagccaattt taaaagtcgg
     9601 tcacttttaa aattggcttt gcgattgctt gcggttgcaa tgcaaccgca agcaatcgcg
     9661 gatttcaagg tagctttttc gaaactcagg gggttttaaa aaagcctcca ttaaaaaaag
     9721 attagggggg tggcagtcaa aaccgcggtt ctgttgtagt ataccgttcc gtgaagatcg
     9781 tcatcgtcgg cgccggcatg gtcggcgcct ccatagcccg ggaactcgta gaggaaaaaa
     9841 aggacgtcat cctcatcgag aaggaccccg agaaagcccg gttcgcgtcg aacgccctcg
     9901 actgcctcgt cctgaacgag gacggcatcg acccggaaac cctgaaacga gccggtacgg
     9961 aggatgccga ctggttcatc tccctgatgg ggaccgacga ggcgaacatc gtggcctgcg
    10021 gcgtcgtctc ctccgccttc cccaagcccc ggaccatcgc ccgtgtgcgc agctcctact
    10081 atggctccct gggcgccgcg agcaagagtt tcctgggggt ggatcatgtg atcaaccccg
    10141 agatcgaggc cgcccagacc atcgtccagg ctgcccggga agggatctcc ggggagatcc
    10201 tgcccctgcg ggaagcccgg atacgccttc gatccatccc ggccgccgag gatccccgct
    10261 tccccggaca caccatccag gagatccgca aggccgtcgg gctggacttc ctgatccccg
    10321 cggtcgtccg ggacgggggc ctgacccttc ccgccgggga cttcgagatc cagggggacg
    10381 acctggtcta ccttctggcg gagcccaagg tcctggacgt catcctgggt cccgccgcgg
    10441 aaccgcagag ccaggtccgg aaggtcctga tcgtcggggc gaccaccgtc ggagagcggg
    10501 tcgtggaagg cctgctgcgc tccggccagg agaccaaacg gccctgggga aagctctttt
    10561 ccgccctggc cgggcattcc gggccggaga tcacggtcat cgacgacaac cgggagcacg
    10621 ccaaacgctt cgcccaccgc tttccctccg tcaccatcgt gaaccgagac ctggtggagg
    10681 aagacgccct ggagcgggaa ggcgccggta acgcggatct ggtcgtgtgc gtgacggggg
    10741 cccagagcta caacatcctc accgcccggc tggccaagga actcggggcc cggcgtgcca
    10801 tagccctgct cctgaacaac tccttcatca agctggcccc gaagctggag ctggacgccc
    10861 tggtcagcat gcggaccgtc gtcggctcct cgatcctgaa catcatccgc aaggccaaca
    10921 tccggaccct ccacgccttc cacgaggacg acgtggagct ggtggaactc tcgatccagg
    10981 aggaatcccc cgccgcgggc cggaaggtac gcgacctggg cctcccgaag ggcgccctgg
    11041 tagccttcgt cctgcatggg acctccagcg ccatccccac cggcgacacc ctcgtccagg
    11101 tcggggacac ggtgggaatc ctgacgaaga aaggcttgat cgagcgcctg gaggaggtgt
    11161 tcggggggcg gcgtggccgg taaacccctg ctgcgcatcc tggccatcct cctgggcatc
    11221 gtctccgcct tcatggccct ctgtctggcc gcgggattcc tgatggggga aggcgaccgg
    11281 accctgcggg ccttcgccat ccccgtgggc ataggggccg cggggctggc cctggcctgg
    11341 atcggcaagg tcacccgccg caggatcagc ctgccgcccc gggacgggtt cctcttcgtg
    11401 gccctggcgt gggtcttcgc ctccgccctg ggcgccctgc ccttcaccct cgcgggcacg
    11461 atgagctacg tggacgcctt cttcgagacc atctcgggct tcacgaccac cggggcctcc
    11521 atcctttcgg acatcgaggt catccccaag tccctgctcc tatggcggtc caccacccac
    11581 tggctggggg gcatgggcat cgtggtcctc acggtggcca tcctgcccct cctgggcgtc
    11641 ggcgggctcg gtctcatgga ggccgaggcc ccgggcccct cggtggacaa gatcaccccc
    11701 cggatatcct ccacggcaaa gatattgtgg ctgatctacg tggccctcac ggtcctggag
    11761 atcctcctgc tgatggccgg ggggatggac tggttcgacg ccgtcaccca caccttcggg
    11821 accatggcca ccgggggatt ctcgaccaag aacctcagcg tcggccacta tcgttccggc
    11881 tacatcgacg tcgtgatcac ggtgttcatg ctggccgcgg gtctcaactt cagcctctac
    11941 ttcaagttac tgtccgggaa ggcgatctcg gcgttccggg acaccgaggc ccggacctac
    12001 ctgggcatct tttccgtcgc cgccctcctg atcgcctggg atctgtggcg ggcggggacc
    12061 ttcggggggt tcggcgcctc cctgcgctac gcggggttcc aggcttcctc catcatcacg
    12121 accacggggt tcgccaccgc ggatttcgcc aaatggccgc ccctctccca aacgatcctg
    12181 ttcctgctca tgttcgtcgg cggatgcgcg ggatccaccg gagggggcat caaggtcatc
    12241 cggatcgtct tcctgtccaa gatggccttc acggagatga agtacctgct gaatccccgg
    12301 ggcatctacg ggatcttcgt ggacgggaag tccctgcgga agaacatcat ctacgacacc
    12361 gcggccttcg tgctcctgta catcttcctg ctcctggcca cgaccctggt ggtctccgcg
    12421 ggcgggttcg acatcctgac ctccctgacg gccagcctgg ccacggtggg gaacatcggc
    12481 cccggattcg gcaaggtggg gcccgcgttc aactacgggt tcttcccgga ctggatcaag
    12541 ctgtggctgt ccttcgccat gctggcgggg cggctggaga tctacaccat cttcattctc
    12601 ttcacccggg cgttctggcg ccggtaagga cgcgtccccg gcgttagagg gcggggcggt
    12661 ccgcggcgcg ccggtccacc cgggaaagga actcctcggg caggatcacg tgctccttgg
    12721 tctgggcgct ggtggccgcc cagcggatcg cgccgtcctt gagcatgcgg aatagggcgg
    12781 cctccaggtc gtccagggca atccccatcc gcccgttgat ccaggccttc atgtccccca
    12841 ggtgcgcctt gatcccgccg tcgtggatcc gctcgccggt ccgcagcgcg aaatccgcca
    12901 tgcccctctg gatgcgctcc ttgggcaggg tctcgatcag gtccgacacg aggtcgttgg
    12961 aggaccgtat ccgctccgag aggaccttga tgatcttgag ggcgaacctg ccgttcgaga
    13021 ggatcatggc ttcgaaggcc ggacggtcca ccaccaggag cttggtatcc ttgaccgcca
    13081 cggcggaggc ggaccggggc cggccgtcga tcagggccat ctccccgaag aactcgttcg
    13141 gctcgtccac ggtggacagg acggtctccc cgccctcgac ctggtcgag
//
LOCUS       DAOTEI010000095        13118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3296_length_13118_cov_5.007732, whole genome shotgun sequence.
ACCESSION   DAOTEI010000095 DAOTEI010000000
VERSION     DAOTEI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_13118_cov_5.007732"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1195
                     /gene="obgE"
                     /locus_tag="P5313_10065"
     CDS             29..1195
                     /gene="obgE"
                     /locus_tag="P5313_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRY80750.1"
                     /translation="MIKFADEADITVSSGKGGNGCIAFRREKYVPRGGPAGGDGGRGG
                     DVVFEVRRNLRTLAHLRYQQTYKARGGEDGTSRNMHGSDGADAVIPIPPGTLVKDAET
                     GEVLRDFTEETDRWVFLKGGNGGWGNTHFKTSTNQAPRIALDGKPGRTVRLRVELQLL
                     ADIGFVGFPNAGKSSLLDHFTNARPKIAPYPFTTKIPNLGMLTVDDRDIILADIPGII
                     EGAHEGHGLGIRFLKHISRASALAFLVDLSDDSFRDAFPILLSELREFSADLTAKPRI
                     VVGTKLDLPGTAERLAELRAALPAERVFGISVFNREGIPALRSEFLDFVLEAERAQEA
                     ARAASSAEREPGAGGVRAPGAPTAPDSRVPGPAETGSPGPGTVWTATAGWKDGD"
     gene            1198..1773
                     /gene="nadD"
                     /locus_tag="P5313_10070"
     CDS             1198..1773
                     /gene="nadD"
                     /locus_tag="P5313_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRY80751.1"
                     /translation="MRLLMFGGSFNPVHIGHLILAEEARIALGYTHVALVPAALRPLK
                     DIQDDPGPEARLAMTRAAAADDDRLVVWDGEIRRGGLSYTIDTIRELARSFELEGKPG
                     LLVGDDLLPGFSEWRQADALAREARIVCARRERADRVAFPYDHVYLDNLLIPVSSRMI
                     RERIAAGQPYRRLVPDPVWRIIEENGYYRRG"
     gene            1766..2353
                     /gene="yqeK"
                     /locus_tag="P5313_10075"
     CDS             1766..2353
                     /gene="yqeK"
                     /locus_tag="P5313_10075"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HRY80752.1"
                     /translation="MDEPGLDGLPERLEEAARSLLSPNRFEHSRGCALFAAEICVRSG
                     IDPRKGSAAGWAHDMARGLPEELQERLARDCPVPVPVSVFANPVLLHGPAAATLLARD
                     YGVRDRDILEAAALHTVGRADMGRLAKIVWAADKLEPGRRHVDDALRERCRGLDPDSL
                     ILEALEATIAWLETRGREVAPETRDLYNTLRKERG"
     gene            2350..3528
                     /locus_tag="P5313_10080"
     CDS             2350..3528
                     /locus_tag="P5313_10080"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRY80753.1"
                     /translation="MKHVTLDRSSIFLILIILFIAACVGFLLLRLRADSLDEALKNDR
                     ILNIAFVVERGGKPAATEIFLYYPMNQRGALLDVPAETGLILKSLNRVDRIEALYDPQ
                     NPKAYIEEVSRLVGAPLPFHIILRESGLIELVDLLDGLSLFIPNPVDARGPSGRVLLP
                     SGSVTLDGSKVAAFSSWSDPEAPELERVERRQRLLQALLKRVGERRDYVLSPEVFPEI
                     SRRMRTNIESDALYRLFERIADLDVDRLVTQRITGNYRSMDGATLLFPHYDGSLVKDI
                     VKQTLNALVNADSFAVADKVFTIEILNGTTVRGLAGKTAEIFQSFGYEVGSVDNAETQ
                     DLARTVIYDRYSNGEAARTIGNVIRCKNVVPGDGGRPESFADFTIVLGRDFNGRFCDE
                     "
     gene            3518..3913
                     /gene="rsfS"
                     /locus_tag="P5313_10085"
     CDS             3518..3913
                     /gene="rsfS"
                     /locus_tag="P5313_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRY80754.1"
                     /translation="MTNDHLSEVLDCARVLADHNGQETLVLDLTELSLWTDYFVITTA
                     TSSTHLAGLVRHLTEHLAVRGLEPSRKPRLADDEEWCLVDAGWFVVHVMSVRARGFYE
                     LEKLWFQAKAISVHPSGPGAPAGNPTGTA"
     gene            complement(4013..4324)
                     /locus_tag="P5313_10090"
     CDS             complement(4013..4324)
                     /locus_tag="P5313_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80755.1"
                     /translation="MTPSNGESALDPNLSLLGVEPLDPSPDNPFLRFGDGYDEDYEDD
                     FEEEEEYDDLDDEDFDEGDEDFEDEEDDFDEDLDDDFDEESNGDDYEYEDDLDYDDFD
                     E"
     gene            4568..5419
                     /gene="nadC"
                     /locus_tag="P5313_10095"
     CDS             4568..5419
                     /gene="nadC"
                     /locus_tag="P5313_10095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRY80756.1"
                     /translation="MDDTHFGALLGMALEEDLDGAGDVTCRAVFSEGDRCAAVLTSKD
                     TGILCGIERFREVFRRVDPSAAVELLAADGDRLSPGMEVARIEGKTRSILEAERTAIN
                     FLSFLSGIATAASEYVRAAREKGKAVVLDTRKTLPGYRSLSKYAVRTGGGANHRMGLH
                     DMVLIKDNHIDAAGGIPRAVERVRARWGREFRVEVECRTVEEVRQAVEAGADVVMLDN
                     MDFETARRALELRAPGVSFEASGGISLETAGAWSALGVDYISVGRLTHSVRGFDFSLT
                     IRKEEAP"
     gene            5416..6996
                     /gene="nadB"
                     /locus_tag="P5313_10100"
     CDS             5416..6996
                     /gene="nadB"
                     /locus_tag="P5313_10100"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRY80757.1"
                     /translation="MSSRHESFDVIVVGAGIAGLSAAVRAAEAGASVCVLTKEAAPEE
                     CNTRYAQGGIVARGGPEDPELLEQDILAAGCWLNNREAVRIVSREGPGAVEDLLLRKA
                     QVPFLRNAEGEVDLTREAAHSIRRIWHVKDYTGRSIQESLTAYVRGIPNIHIFTSCMA
                     VDVITNTHHSRDDSQRYAPKRALGVYVYGENTGEVVPFFASATILAAGGIGNLYLHTS
                     NPPGATGDGVAMAERAGAEVINAEYVQFHPTILFHRDLKGFLISESLRGEGARLMNRK
                     GEYFMDRYHPALRDLAPRDEVSRAIYYEMEQSESTYVLLDTSLLKVDPRDRFPSIHET
                     CLRAGIDMTRDPIPVVPAAHYFCGGVKTDMEGRTTIPGLYAAGETACTGVHGANRLAS
                     VSLLEGLVFGLRAGTAAAALADRPGLPLVQSIPDWVFPAEEEAADPILIYHDLLNIQM
                     TMWDYAGIVRTRKRLARAKADLNYLAHRVDSFYRQARLSRPLLELRNAVDTASRIVRD
                     AEANPKSLGCHYLRGSPV"
     gene            7031..7933
                     /gene="ispE"
                     /locus_tag="P5313_10105"
     CDS             7031..7933
                     /gene="ispE"
                     /locus_tag="P5313_10105"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRY80758.1"
                     /translation="MVRTVVVEAPAKINLHLQVLGKRPDGFHDILSLFQAVSLCDTLD
                     IRIRPSGDVRVFGEFDCPEPENTIHRAARSFLAASGRPEGAEIRAVKRIPAGAGLGGG
                     SSDAASVLRALNRAFGDTLSGSELARLAAGIGSDVPFFLGTPCAVVGGRGEILEPADA
                     RPDYGLVILFPGFPIGTAWAYAAVDESRKAAPSGGDETWDFGRLREEYRRDPESWTFR
                     NDFFDALAPGNAELRRAKRALEDEGASFAGMTGSGSAFFGIFGSETAADRAARNLNER
                     EGGKGRWSAAAAAPLARMPVPRLQ"
     gene            8004..8282
                     /gene="spoVG"
                     /locus_tag="P5313_10110"
     CDS             8004..8282
                     /gene="spoVG"
                     /locus_tag="P5313_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HRY80759.1"
                     /translation="MEITDIRIRKVTSEGKLKGYVTVTFDDCFVVHNVKVIEGKTGVF
                     IAMPSRKTRTGEYKDIAHPICPDFRQKLQDKILDAFGNPENFEEEPAT"
     gene            8308..8379
                     /locus_tag="P5313_10115"
     tRNA            8308..8379
                     /locus_tag="P5313_10115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8340..8342,aa:Gln,seq:ttg)
     gene            8417..9079
                     /locus_tag="P5313_10120"
     CDS             8417..9079
                     /locus_tag="P5313_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRY80760.1"
                     /translation="MSQTLLTAVSRPTATKGQVRRLRAEGKLPAVIYNRHGQSRPILL
                     DSAEFAKAVTGVSESTILKLSIDGKTTEAFIKDRQVNWLRGTLLHVDFFEVEQDRAIR
                     AHVSVHLLGVPVGVREGGILEAPVHEVEVECLPKDLPGKIELDVSNLGANHSIHVRDI
                     SLPNVRILNSGDQVVALVKFAKAEAAPAVEEAAPAEGEAAATPAAGAAPGAAPAAPEK
                     KE"
     gene            9205..10557
                     /gene="tilS"
                     /locus_tag="P5313_10125"
     CDS             9205..10557
                     /gene="tilS"
                     /locus_tag="P5313_10125"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRY80761.1"
                     /translation="MVAFSGGSDSTALLLAVATVAPALDITVRAAWVDHGIRPASERA
                     RERNFVEALTARLGIPCRITEAPEDPLEARAARLKTSLEAEARDFRYAALRAEADRAG
                     CQRILTAHTRDDQAETLLARIFSGSGAGGLRGIPEDRPPFLRPFLSLPKAALIRYLED
                     RGQDWREDSTNAGDDYQRNRIRRRLIPAVEKAFPGFRKALAALAEKSRLDEDLLSALS
                     RSELPASRSEGQSSFDARAFHAAHPALRLRALTAEASRLAGPGRRVPYALLRAAVFAD
                     PGQPGSTAPRILAQGTGLRFAEEDGRILVTPVSGGVTIPGGYSFQFERPGTLRIDTGG
                     SCTVYFRPGSAGPAEGTFEFPLVVRSRLPGDRIAIRDGRKPLDELLAEWKVPREWREY
                     VPVLEDRSGIVGILGSRAGARDRYRPGPASLDASVPRLAVEIEGIGPFRSIERSAEAP
                     "
     gene            10554..12419
                     /gene="ftsH"
                     /locus_tag="P5313_10130"
     CDS             10554..12419
                     /gene="ftsH"
                     /locus_tag="P5313_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRY80762.1"
                     /translation="MSPDNDKNEQPPKRPLDGPPRGNRFALILFIALMGAFSVFFLTD
                     QKSSVNEISYSAFLSYLEQGTVEAVKIVDQTEIEGKLRGKTGNTALFRTRIPYTDLDL
                     VKTLRESGVSIEGAVRGVSVLQVLLETLPWIFGFFLIWMMLRQFQGNNKAFSFGKSRA
                     KRYLDASKKTTFEDVAGQKEAKYELTEVVDYLKNPQKFVKMGARIPKGVLLVGMPGTG
                     KTLLAKAVAGEANVPFFHMSGSDFVEMFVGVGASRVRDLFEQGRKSAPCILFIDELDA
                     VGRTRGAGYGGGHDEREQTLNQMLVEMDGFDTKDGVIILAATNRPDVLDPALLRPGRF
                     DRQVVVAMPDVQEREQILAIHMNKIPVETGTDVKRLARATPGTSGADLANLVNEAALF
                     AVRRNKSVVGMEDFEDARDKILMGIARSSLFLTEEEKLATAYHEAGHALVHYYLDHTD
                     PLHKVTIVPRGRALGLALSLPEKDTYSHSVARLKDRIVVCYGGFAAESIVYGETTTGT
                     AQDLRQATDIARQMVCEWGMSPKMGAVAYGQEDEPIFLGKEIARHKDYSEETARNIDA
                     SVRSILDEGRDRALSILTDHKDQLETLARELIQKETLQDDAIRVLLGFPAREIKE"
     gene            12422..>13118
                     /locus_tag="P5313_10135"
     CDS             12422..>13118
                     /locus_tag="P5313_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80763.1"
                     /translation="MRRAIAPAVLLSCAAFAFSQAPGFPDLNVRQSAARAAVERAVRA
                     LEAGDAEGAREASDEARYLDPGNSDARYLLAAADLRGGRPTPEAEGELRTALAGGTFL
                     RYTADDAARLLAHILVRTKRYSEALALASRPGLAGDSDALYLRVRALRFLGDPDAFLR
                     AVDAGLDRFPSDARIPRELLAFTARTPRTEPTAARVGRVEARLGYYRFLDPEILSLLA
                     PFRPDPEARRDLIL"
BASE COUNT         2144 a         4479 c         4404 g         2091 t
ORIGIN      
        1 gtattccggg gcgccgcgga ggcacgggtt gatcaagttc gccgacgagg ccgacatcac
       61 ggtatcctcc ggcaagggcg ggaacggctg catcgccttc cggagggaga agtacgttcc
      121 ccggggcggt cccgcgggcg gcgacggggg acggggcggg gacgtggtct tcgaggtgcg
      181 ccgcaacctg cggaccctgg cccacctccg ctatcagcag acctacaagg cccggggcgg
      241 cgaggacggc accagccgga acatgcacgg gtccgacggg gccgacgccg tcatccccat
      301 ccccccgggc accctggtca aggacgccga gaccggggag gtcctccggg acttcacgga
      361 ggagacggac cgctgggttt tcctcaaggg tgggaacggg ggctggggaa acacccactt
      421 caagacctcc acgaaccagg cgccccggat cgccctggac gggaagcccg ggcggaccgt
      481 ccggctccgc gtggagctcc agctcctggc ggacatcggc ttcgtgggct ttcccaacgc
      541 gggcaagagc tccctcctgg accatttcac gaacgcccgg cccaagatcg ccccgtaccc
      601 cttcacgacc aagattccca acctcgggat gctgaccgtg gacgaccggg acatcatcct
      661 ggcggacatt cccgggatca tcgagggggc ccacgagggg cacggcctgg ggatccgctt
      721 cctcaagcac atctcccggg cctccgccct ggccttcctg gtcgaccttt cggacgattc
      781 cttccgggac gcgttcccga tcctgctctc ggagctccgg gaattctccg cggacctcac
      841 ggccaagccc cggatcgtgg tggggaccaa gctggacctc cccggaacgg cggagcgcct
      901 cgcggagctc cgggccgccc tgcccgccga gagggtcttc ggcatctcgg tcttcaaccg
      961 ggagggcatc cccgccctca ggtcggagtt cctggacttc gtgctcgaag ccgagcgggc
     1021 ccaggaggcc gcccgggcag cctcctccgc cgaaagggaa ccgggggccg gcggggtccg
     1081 ggcgcccgga gcgccgacgg cgccggattc cagagtcccc ggaccggcgg agacgggttc
     1141 ccccgggccg ggaaccgtct ggaccgccac ggcgggctgg aaggacgggg actagccgtg
     1201 cgcctcctca tgttcggggg gagcttcaat cccgtccaca tcgggcacct gatcctggcc
     1261 gaggaggccc gcatcgccct gggctacacc cacgtcgccc tggtgcccgc cgccctccgg
     1321 cccctgaagg atatccagga cgatcccggt ccggaggccc ggctggccat gacccgggcc
     1381 gccgcggcgg acgacgatcg cctcgtcgtc tgggacgggg agatccgccg gggcgggctt
     1441 tcctacacga tcgacaccat ccgggaactc gcccgatcct tcgaactgga ggggaagccg
     1501 ggtctcctgg tcggggacga cctcttgccc ggcttttccg agtggagaca ggcggacgcc
     1561 cttgcccggg aagcccggat cgtctgcgcc cgacgggagc gcgcggaccg ggtggccttc
     1621 ccctacgacc acgtctacct ggacaacctc ctcatacccg tctcctcccg gatgatccgg
     1681 gagcggatcg cggcgggaca gccttaccga cggctggttc ccgatccggt ctggcgcatc
     1741 atcgaagaaa acgggtacta ccgccgtgga tgaaccgggt ctggacggac ttccggaacg
     1801 gttggaagag gcggcgcgga gtcttctgag ccccaaccgg ttcgaacaca gccggggctg
     1861 cgcccttttt gccgcggaaa tctgcgtccg gtccgggata gatccccgga aaggttcggc
     1921 agcaggatgg gcccatgaca tggcccgggg acttcccgaa gaacttcagg agcgcctggc
     1981 ccgggactgt cccgtgcccg tcccggtcag cgtcttcgcg aacccggtgc tcctgcacgg
     2041 cccggccgcc gcgaccctcc tggcgcggga ttacggcgtc cgggatcggg acatcctgga
     2101 ggcggcggcc ctgcacacgg tcggtcgggc ggacatgggt cgcctggcca agatcgtctg
     2161 ggccgcggac aagctggaac ccggccggcg gcacgtggac gacgcgctcc gagaacgatg
     2221 cagggggctc gatccggatt ccctcatcct ggaagccctg gaggcgacca tcgcctggct
     2281 ggagaccagg ggccgggagg ttgcgccgga gacccgggat ctgtacaata ccctgcgaaa
     2341 agagcgcgga tgaagcacgt taccctggat agaagctcca ttttcctgat cctgatcatc
     2401 ctcttcatcg cggcctgcgt cggcttcctg ttgctccgac tccgggcgga ttccctggac
     2461 gaggccctga aaaacgaccg gatcctcaac atcgccttcg tggtggagag gggagggaag
     2521 ccggccgcca cggagatctt cctctattac cccatgaacc agagaggggc cctgctggac
     2581 gtgcccgccg agaccgggtt gatcctcaag tccctgaacc gggtggaccg catcgaggcc
     2641 ctctacgacc cccagaaccc caaggcttat attgaagaag tctcccggtt ggtcggggcc
     2701 cccctcccgt tccacatcat cctccgggaa tcgggcctga tcgaactcgt ggacctcctg
     2761 gacgggcttt ccctgttcat ccccaacccc gtggacgccc ggggcccctc cggccgggtc
     2821 ctccttccct cgggatccgt gaccctggac gggagcaagg tagccgcctt ctcctcctgg
     2881 tccgatcccg aggcgccgga gttggagcgg gtggagcggc gccagcgtct cctgcaggcc
     2941 ctgctgaagc gggtcgggga gaggcgggac tacgtgctct cccccgaggt gttcccggag
     3001 atatcccgcc ggatgcggac gaacatagag tccgacgccc tgtaccgact gtttgagagg
     3061 atagcggatc tcgatgtgga ccgcctggtc acccagcgaa tcacggggaa ctaccggtcc
     3121 atggacgggg cgaccctcct cttcccccat tatgacggga gcctggtcaa ggacatcgtg
     3181 aagcagaccc tgaacgccct ggtgaacgcg gacagcttcg cggtggcgga caaggtgttc
     3241 acgatcgaga tcctgaacgg caccaccgtc cggggcctgg cgggaaagac cgccgagata
     3301 ttccagagtt tcgggtacga ggtgggttcc gtggacaacg cggagaccca ggacctcgcc
     3361 cgcacggtca tctacgaccg gtattccaac ggcgaagcgg cccgcaccat cggcaacgtg
     3421 atccgctgca agaacgtggt gcccggggac ggcggccggc cggagtcctt cgcggacttc
     3481 acgatcgtgt tgggccggga tttcaacggg aggttctgtg acgaatgatc atctctccga
     3541 ggtcctcgac tgcgcccggg tgttggcgga ccacaacggc caggaaaccc tggtcctgga
     3601 cctgacggag ctctccctct ggacggacta cttcgtgatc acgaccgcca ccagctccac
     3661 ccacctggcc ggcctggtcc ggcacctgac ggagcacctg gccgtccggg ggctggaacc
     3721 ctcccggaag ccccgcctgg cggatgacga ggagtggtgc ctggtggacg ccgggtggtt
     3781 cgtggtccac gtcatgagcg tgcgggcccg gggcttttac gagctggaga agctctggtt
     3841 ccaggccaag gcgatctccg tgcatccttc cgggcctggg gccccggccg gaaaccccac
     3901 cgggacggcc taggggcccg ttccggcagc gggaaaggcc gccgggcata aaaaaggagc
     3961 ttccgggcgg ccctgagggc ctccggaagc tcggtatcgc gacgggaatc cgtcactcgt
     4021 cgaagtcgtc gtagtccagg tcgtcctcgt attcgtagtc gtccccgttg gattcctcgt
     4081 cgaagtcgtc gtccaggtcc tcgtcgaaat cgtcttcctc gtcctcgaag tcctcgtccc
     4141 cctcgtcgaa gtcctcgtcg tcgagatcgt cgtactcctc ttcctcctcg aagtcgtcct
     4201 cgtaatcctc gtcgtacccg tccccgaacc ggaggaaggg gttgtccggg ctcgggtcga
     4261 ggggctctac gccgaggagg gaaaggttcg gatcgagcgc cgattcgccg ttggacggag
     4321 tcatatcgta ttcctcccga agaatcctta gttccgcgtg atgaacgggt tgccggaccg
     4381 tgaagccaga aaggtatggt tcggaaccat ggttccatcg aaaatctaaa agagacttgt
     4441 cggttctgtc aactacgttt tcgagataaa aagaaaaata ccgaaggcgg acggtttccg
     4501 cctagttcgc ggttccatat cgttttccgt tgatcttcgg ggggttccgg ggctaggatg
     4561 gctcaccatg gacgataccc atttcggtgc cctcctgggc atggccctgg aggaggatct
     4621 ggacggcgcg ggggacgtca cctgccgggc cgttttttcc gagggagacc gctgcgcggc
     4681 ggtcctgacg agcaaggata ccgggatcct ctgcgggatc gagcggttcc gggaagtctt
     4741 ccggcgcgtc gatccctcgg ccgcggtcga gctcctggcc gcggacggcg accggctctc
     4801 cccggggatg gaggtggccc ggatcgaggg aaagacgagg tccatcctgg aagcggaacg
     4861 gacggccata aacttcctgt ccttcctttc cgggatagcc acggccgcct ccgagtacgt
     4921 ccgcgccgcc cgggaaaagg gaaaggccgt cgtcctggac acccggaaga ccctgcccgg
     4981 ctaccggtcc ctgtccaagt acgccgtccg gacgggcggg ggggccaacc accgcatggg
     5041 gctccatgac atggtcttga tcaaggacaa ccatatcgac gcggcggggg ggatcccccg
     5101 ggccgtggag cgggtacgcg cccggtgggg ccgagagttc cgcgtcgagg tggagtgccg
     5161 caccgttgag gaggtgcgcc aggccgtcga ggcgggcgcg gacgtcgtga tgctggataa
     5221 catggacttc gagacggccc gcagggccct ggaactccgg gcaccgggtg tttccttcga
     5281 ggcctcggga gggatttccc tggaaaccgc gggcgcctgg agcgccctgg gggtggatta
     5341 catctccgtc ggccgcctga cccattccgt acggggcttc gacttctccc tcaccatacg
     5401 gaaggaggag gcgccgtgag ctcccggcac gagtccttcg acgtgatcgt ggtgggagcc
     5461 gggatcgcgg ggctttccgc ggccgtccgg gccgcggagg ccggggcctc cgtatgcgtc
     5521 ctcaccaagg aggcggcgcc cgaggagtgc aacacccggt acgcccaggg gggcatcgtc
     5581 gcccggggcg ggccggagga ccccgaactc ctggaacagg acatcctggc cgccggttgt
     5641 tggctgaaca accgggaggc ggtacgcatc gtcagccggg agggtcccgg ggccgtggag
     5701 gatcttctcc tccggaaggc gcaggttccc ttcctgcgca acgcggaggg ggaagtggac
     5761 ctcacccggg aggccgcaca ttcgatccgg cgcatctggc acgtcaagga ttacacgggc
     5821 cgctcgatcc aggagtccct gaccgcctat gtccggggca tccccaacat ccatatcttc
     5881 acctcctgca tggccgtgga cgtcatcacc aacacccatc attcccggga cgactcccag
     5941 cgctacgccc ccaagcgggc cttgggggtc tatgtctacg gggagaacac cggggaagtg
     6001 gtccccttct tcgcttcggc gaccatcctg gccgcggggg ggatcggcaa cctctacctc
     6061 catacctcca atcccccggg ggccaccggg gacggcgtag ccatggccga acgggcggga
     6121 gcggaggtca tcaacgcgga atacgtgcag ttccacccca ccatcctctt ccaccgggac
     6181 ctgaagggct tcctgatctc cgagtccctc cgcggggagg gcgcccgcct catgaaccgc
     6241 aagggggagt acttcatgga ccggtaccat ccggccctga gggacctggc cccccgggac
     6301 gaggtttccc gggccatcta ctacgagatg gagcagtccg agtccaccta cgtcctgctg
     6361 gacacctccc tgctcaaggt ggatccccgg gaccggttcc ccagcatcca cgagacctgc
     6421 ctgcgggccg ggatcgacat gacccgggat cccatacccg tggtgcccgc ggcgcactac
     6481 ttctgcgggg gcgtgaagac ggacatggaa gggcggacga ccatcccggg actctatgcg
     6541 gccggtgaga cggcctgcac gggggtccac ggggccaacc gactggcctc ggtgtccttg
     6601 ctggaaggct tggtcttcgg cttgcgggcc ggaacggccg cggccgccct ggccgaccgg
     6661 cccgggctgc ccctggtcca gagcattccg gactgggtct tcccggcgga agaggaggca
     6721 gcggatccca tcctcatcta ccacgatctt ttgaacatcc aaatgaccat gtgggactac
     6781 gccgggatcg tccgcacccg gaagcgcctg gcccgggcca aggcggacct gaactacttg
     6841 gcccaccggg tggacagctt ctaccgccag gcccgccttt cccggcccct gttggagctt
     6901 cgcaacgccg tggacaccgc ttcccggatc gtacgggacg cggaggcgaa cccgaagtcc
     6961 ttgggctgcc attacctacg gggatcgccg gtttgacacg gaggccgttc ccgtagtatt
     7021 gtggcccgcc atggttagga cggttgtcgt cgaggcgccg gccaagatca acctgcacct
     7081 ccaggtgctg ggcaagcggc cggacgggtt tcacgacatc ctcagcctgt tccaggcggt
     7141 ttccttgtgc gataccctgg acattcggat ccggccttcc ggggacgtcc gggtcttcgg
     7201 ggagttcgac tgcccggagc ccgagaatac catccaccgg gccgcgcgct ccttcctggc
     7261 ggcttccggc cggccggagg gcgcggagat ccgcgccgtc aagcggatcc ccgcgggggc
     7321 cggcctcggg ggagggtcca gcgacgcggc gtcggtcctc cgggccctga accgggcctt
     7381 cggggacacg ctttccggat ccgagctcgc ccgcctggcg gccgggatcg gatcggacgt
     7441 gccgttcttc ctgggcaccc cctgcgccgt ggtcggcggc cgaggcgaga tcctggagcc
     7501 cgcggatgcc cggccggatt acggtctggt gatccttttc ccgggattcc cgatcggcac
     7561 ggcctgggcc tacgcggcgg tggacgaatc ccggaaggcg gccccctccg ggggggacga
     7621 gacctgggat ttcgggcggc tccgggagga gtaccggagg gatccggaat cctggacctt
     7681 ccggaacgac tttttcgatg ccctggcccc ggggaacgcg gaactccgta gggccaagcg
     7741 ggccctggag gacgagggcg cctccttcgc cggcatgacc ggttcgggga gcgccttttt
     7801 cggcattttc ggatcggaaa ccgcggcgga tagggccgcg aggaacttga acgaacggga
     7861 gggtggcaaa ggccgatgga gcgcggcagc cgcggcgccg cttgcgcgca tgccggtccc
     7921 gaggttacaa taaatactgc gtttccgccc gcgaaagcgg gggccccgcc cgtgggggga
     7981 tcctcgtcaa ggagaggcat cgtatggaga tcacggatat ccgcatccgg aaggtgacca
     8041 gcgaggggaa gctcaagggc tatgtgaccg tgacgttcga cgattgtttc gtggtccaca
     8101 acgtgaaagt gatcgaaggc aagacgggag tgttcatcgc catgccgagc cgcaagaccc
     8161 ggaccggcga gtacaaggac atcgcccatc cgatatgccc ggatttccgg cagaaactcc
     8221 aggacaagat cctggacgcc ttcgggaatc ccgagaattt cgaggaggaa ccggccacat
     8281 aaggcccctc cggaccgctt gcatccgtgg gacgtcgaca agtggtaagt cagcgggctt
     8341 tggttccgcc attcgcaggt tcgaatcctg ccgtcccagt aacgggtatc caccgtacca
     8401 tcaaggagta tagtacatga gccagacatt gttgaccgcc gtttcccgcc cgaccgccac
     8461 gaagggtcag gtccgccgcc tgcgcgcgga gggcaagctc cccgcggtca tctacaaccg
     8521 ccacggacag tcccgcccga tcctcctgga cagcgcggag ttcgccaagg ccgtgaccgg
     8581 ggtttccgaa agcaccatcc tcaagctctc gatcgacggc aagaccaccg aagccttcat
     8641 caaggaccgc caggtcaact ggctccgggg aaccctcctg cacgtggact tcttcgaggt
     8701 cgagcaggac cgggccatcc gcgcgcacgt ctccgtccac ctcctgggcg tacccgtcgg
     8761 cgtacgggag ggcggaatcc tggaggcccc cgtccacgag gtcgaggtcg agtgtcttcc
     8821 caaggacctg cccggcaaga tcgagctgga cgtctccaac ctcggcgcca accactccat
     8881 ccacgtccgg gacatcagtc tgcccaacgt gcggatcctg aactccgggg accaggtggt
     8941 cgccctggtc aagttcgcca aggccgaggc cgcccccgcc gtggaggaag ccgctcccgc
     9001 cgagggcgaa gccgcagcca cccccgcggc cggtgccgct cccggagccg cccccgccgc
     9061 gccggagaag aaggagtaag cctgaatcgg tcgaccccgc cggattccca gcggggcagc
     9121 cggccggctt tccgccggcg ggacccggtc gagcgccgcc tggcctcctt cctcgcggaa
     9181 cggggcctgg gcggcgtttc cgtcttggtc gccttctcgg gaggctcgga ttccacggcc
     9241 ctgctcctgg ccgtcgcgac ggtggcgccg gccctggaca tcacggtccg cgccgcatgg
     9301 gtggaccacg gcatccgccc cgcctccgag agggcccggg agcggaactt cgtggaagcc
     9361 ctgacagccc gactgggcat accctgccgg atcaccgagg cccccgagga tcccctggaa
     9421 gctcgggcgg cccgcttgaa aaccagcctg gaggcggaag cccgggactt ccggtacgcg
     9481 gccctgaggg ccgaggcaga ccgggccggt tgccagcgca tcctcacggc ccacacccgg
     9541 gacgaccagg cggagaccct cctggcccgc atattctcgg gatccggggc cggagggctg
     9601 cgggggattc cggaggaccg ccctcccttc ctccgcccct tcctttccct gcccaaggcg
     9661 gccttgatcc gctacctgga ggacagggga caggactggc gggaggattc caccaacgcc
     9721 ggggatgact accagcgcaa ccggatccgt cgccgtctga tcccggctgt ggagaaggca
     9781 ttcccgggat tccggaaggc cctggcggcc ctggcggaaa agagccgcct ggacgaggac
     9841 ctcctttccg ccctttcccg gtccgaactg ccggcctcgc gttcggaggg acagagctcc
     9901 ttcgacgccc gggctttcca cgccgcccat cccgccctgc gcctgcgggc cctgaccgcg
     9961 gaggcctccc gtctggccgg gccgggccgc cgcgttccct acgccctgct gcgggccgcc
    10021 gtcttcgcgg accctggtca gcccggttcc acggcgcccc ggatcctggc ccaggggacg
    10081 ggactgcggt ttgccgagga ggacggccgg atcctcgtca ccccggtatc cgggggtgta
    10141 accattcccg gcggctactc gttccagttc gaacgcccgg gaaccctccg aattgatacg
    10201 ggcgggtctt gtacggtata cttccgcccg ggatccgccg gacccgcgga ggggaccttc
    10261 gagtttcccc tggtcgtccg gtcccgcctt cccggggacc gtatcgcgat ccgggacggc
    10321 cgtaagcccc tggacgagct cctcgcggag tggaaggttc cccgggagtg gagggagtac
    10381 gtccccgtcc tggaggaccg gagcgggatc gtggggatcc tgggatcccg ggcgggagcc
    10441 cgggacaggt accggccggg gccggcttcc ctggatgctt ccgtcccccg cctcgccgtg
    10501 gaaatagaag gaataggccc cttccgatcg atagaaagga gcgcagaagc gccatgagcc
    10561 cggacaacga caagaatgag caacccccca aacggcccct ggacggcccc ccccggggca
    10621 accggttcgc cctgatcctc ttcatagccc tgatgggggc tttttccgtg ttcttcctga
    10681 cggaccagaa atcctcggtc aacgagatct cctactccgc cttcctgtcc tatctggaac
    10741 agggcaccgt ggaggccgtg aagatcgtcg accagacgga gatcgagggc aagctgaggg
    10801 gcaagaccgg caacaccgcc ctcttccgga cccggattcc ctatacggac ctggacctgg
    10861 tgaagaccct ccgggagagc ggcgtatcca tcgagggcgc cgtccggggg gtcagcgtgc
    10921 tccaggtcct cctggagacc cttccctgga tcttcgggtt cttcctgatc tggatgatgc
    10981 tccggcagtt ccaggggaac aacaaggcct tttccttcgg caagagccgc gccaagcggt
    11041 acctggacgc ctccaagaag acgaccttcg aggatgtcgc gggccagaag gaggccaagt
    11101 acgagctcac ggaggtggtg gactacctca agaaccccca gaagttcgtg aagatggggg
    11161 cccggatccc caagggcgtc ctcctcgtcg gcatgcccgg cacgggcaag accctcctgg
    11221 ccaaggccgt ggcgggggag gccaacgtcc ccttcttcca catgtccggt tccgacttcg
    11281 tggagatgtt cgtgggcgtc ggcgccagcc gggtccggga cctcttcgag cagggccgga
    11341 agagcgctcc ctgcatcctc ttcatcgacg aactggacgc ggtcggccgg acccggggcg
    11401 cgggatacgg gggaggccac gacgagaggg agcagaccct caaccagatg ctggtggaga
    11461 tggacggatt cgacaccaag gacggggtca tcatcctggc ggccacgaac cggcccgacg
    11521 tcctggaccc cgccctcctg cggcccggcc gtttcgaccg gcaggtggtg gtggccatgc
    11581 cggacgtaca ggagagggag cagatcctgg ccatccacat gaacaagatt cccgtggaga
    11641 cgggcacgga cgtgaagcgc ctggcccggg cgacccccgg caccagcggg gcggaccttg
    11701 ccaacctcgt caacgaggcg gccctcttcg cggtgcgcag gaacaagtct gtggtgggca
    11761 tggaggattt cgaggacgcc cgggacaaga tcctcatggg gatcgcccgg tccagcctct
    11821 tcctgaccga ggaggagaag ctggccacgg cctaccacga ggcgggtcac gccctggtgc
    11881 actactacct ggaccacacg gaccctctcc acaaggtcac catcgtgccc cggggcaggg
    11941 ccctcggcct ggccctgtcc ctgccggaaa aggacacgta ctcccactcc gttgcccggc
    12001 tcaaggaccg gatcgtcgtg tgctacggag gattcgccgc ggaatccatc gtgtacgggg
    12061 agaccacgac cggcacggcc caggatctta ggcaggccac ggacatagcc cggcagatgg
    12121 tctgcgaatg ggggatgtct ccgaagatgg gcgccgtggc ctacggccag gaggatgaac
    12181 ccatcttcct gggcaaggag atcgcccgtc acaaggatta ctccgaggag acggcccgga
    12241 acatagacgc ctccgtgcgg tccatcctgg acgagggaag ggatcgggcc ctttccatcc
    12301 tcaccgatca caaggaccag ctggaaaccc tggcccggga gctaatccag aaggagaccc
    12361 tccaggacga cgccatccgg gtgctgctgg gattcccggc ccgggagatc aaggagtagc
    12421 catgcgtcgc gccatcgcgc cggcggtcct gctctcgtgc gctgccttcg cgttttccca
    12481 ggcgccgggc tttcccgacc tgaacgtccg ccaatccgcc gcccgggccg cggtcgagcg
    12541 ggccgtccgc gccctggagg ccggggatgc cgagggtgcc cgggaagcct ccgacgaagc
    12601 ccggtacctg gatcccggca actccgacgc ccggtacctg ctggccgccg ccgaccttcg
    12661 gggcgggcgg cccactccgg aggcggaggg cgagctccgg accgccctgg ccggggggac
    12721 cttcctccga tacacggcgg acgacgccgc ccggctcctg gcccacatcc tggtccggac
    12781 caagaggtac tccgaagccc tggccctggc atcgcgtccg ggactggcgg gggactcgga
    12841 cgccctttac ctccgggtcc gggcccttcg gttcctgggc gatcccgacg ccttcctgcg
    12901 ggccgtggac gccggcctgg atcgtttccc ctcggacgcc cggattcccc gggagctcct
    12961 ggctttcacg gcccggaccc cccggacgga gcccacggca gcccgggtgg gccgcgtgga
    13021 ggcccggctg ggctactacc gcttcctgga tccggaaatc ctgtccctcc tggccccctt
    13081 ccggccggac ccggaggccc gccgggacct catcctgg
//
LOCUS       DAOTEI010000096        13040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3333_length_13040_cov_4.634732, whole genome shotgun sequence.
ACCESSION   DAOTEI010000096 DAOTEI010000000
VERSION     DAOTEI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13040
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3333_length_13040_cov_4.634732"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="P5313_10140"
     CDS             <1..974
                     /locus_tag="P5313_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80764.1"
                     /translation="YLFTGTWRKPLALDFDARGYGAFAGDSVGVWFQDARAPRLGFWA
                     GTAGSEGILAGGAAALSLLPPGGGFESWFAPEVPAPARLLAAGMVWAGYGFPAGRVIA
                     AGAVSEEDRGGRGWAVRAEGEWGRRGFCASGRISSASPGWRGLDGDAADRWELRTDAS
                     WAVMPRFRLEGRARIGQDPDCGLDWDALARGSWNGKVWTWGAELGACDSARLPPVRLD
                     PAVWAGYETRTVRASARASWVREGADLTRTEVSAALDLGIPRKPGLRLEGARRWTVEG
                     PNWKASATLEVPTARGAWTARAGTSDWAEDGEVVPWELAVVLRSRFP"
     gene            1057..1521
                     /locus_tag="P5313_10145"
     CDS             1057..1521
                     /locus_tag="P5313_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRY80765.1"
                     /translation="MGVPEAREYLRRWNRDGDVLEFDRSSATVELAARALGVEPGRIA
                     KTLSFRGRERPLLVLAAGDARIDNKKFKAAFGLKASMLSPEEALERTGHPVGGICPFG
                     LATELDVYLDESLRRFDHVFPACGSGNSALRCTLAQLEEYSASKAWVDVCSG"
     gene            complement(1824..3065)
                     /gene="ispF"
                     /locus_tag="P5313_10150"
     CDS             complement(1824..3065)
                     /gene="ispF"
                     /locus_tag="P5313_10150"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRY80766.1"
                     /translation="MTGPVAVLITAAGRGERFGGKKETEDLGGRAVLDRAVSAFSRLT
                     GLSSLVITAPRERKSELLTALHARTKSDLERLGDRFVVVPGGPTRRDSVRLGLEALAR
                     ALAPAEAASGSVPALPEAVRDMVVLVHDGARPWLSQGLAERVAAIAGRVGACVPVTGL
                     VETPKEISPDGLVLRHPPRAALANAQTPQGFRLGPLLEAHRRADAEGMEATDDAELWA
                     RYVGPVAWTEGERSNRKITFREDLEFRPETPVEPAAAPFRVGEGWDIHRLAPGRRLLL
                     GGVEVPSDRGEEGYSDGDVLLHAVIDALLGSAALGDIGIHFPPGDPEWKDADSRDLAR
                     RARDLVRRAGWRPVNLDCTVVLERPKLAPHREAIRTSLAELLDLDVEAVSFKAKTMEG
                     LGPVGEGRAVEARAVLLAVRE"
     gene            complement(3062..4117)
                     /locus_tag="P5313_10155"
     CDS             complement(3062..4117)
                     /locus_tag="P5313_10155"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRY80767.1"
                     /translation="MRTDAGTGARRPREISYAQAVREALREELRRDPRVFLMGEDVGI
                     YGGTFGVSAGLIEELGPDRILDTPISEAVLAGAAAGAALTGTRPIVEIPFSDFLSLAL
                     DQIGNQAAKMRYMFGGAVRVPLVVRSPAGSGTGAGAQHSQSQEAVFCHFPGLKVVVPS
                     TPYDAKGLLKTAVRDDNPVVFLEQKLLYKETGPVPEEEYLVPFGKADVKRPGSDLTIV
                     AWGRMVGMCLAAAERLESEGVSVEVLDPRTLVPLDTRAIVSSVAKTGRLLIVHEAVRT
                     GGFGGEIAALVADSEAFPLLKAPIRRVAGFDIPVPYSPNLERNAVPTPERIEDAVRDL
                     LVQGEKRPAGRTGSVRS"
     gene            complement(4107..5066)
                     /locus_tag="P5313_10160"
     CDS             complement(4107..5066)
                     /locus_tag="P5313_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017549999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRY80768.1"
                     /translation="MGYPDSLLEDLYRRMLRIRYFEEEIHYHISRGHIHGTTHLYVGE
                     EAIAAGAAANLSDLDYITSTHRCHGHAVAKGTALSGLAAEMFGKRAGVCGGKGGSMHL
                     ADPARGNLGGTGIVGGGLPIAVGAALSIRRLGQDRVAVCFFGDGAMNEGTFHESVNLA
                     SVWKLPVLFLCENNQYGVSTHVRRSTNVQDLSLRAAAYGIPGRKVDGMDVLAVYEAVR
                     EAKEYVKTNGPLLLVAQTYRFLGHSRSDANVYRTEEEIASWRRRDPIPRFRERLNEEG
                     SFSEDRIEDIEEEVRREVDAAFRYARACEEPGLEDLLRDVYAD"
     gene            5254..6243
                     /locus_tag="P5313_10165"
     CDS             5254..6243
                     /locus_tag="P5313_10165"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HRY80769.1"
                     /translation="MGEDAGRRTEDSIQAADGRPDGSHSLLAKIAHLYYIENLSQKDI
                     SQRTRMSVASVSRALARARELKIVSISIQGGENDFSRLEIEIERTFDLPECLLVPSYG
                     RIEHTYQGLAAAAADLLSRTLKPGAVLGVSWGDTLRSVGESLPRMPSVRADVIPIIGA
                     LGWIETGIYPNSIARTFAQKLGGSSYLVNTPAFVDDPETRKALMEDSNFRPVRDIWSR
                     LDMAFLGVSALTVDSSMYRSGIFTEPDLEGMRTAGGECISNFVVFDGRGRMVPTSVSD
                     RMVYLPLEDLRRVRTVAIAAAGAAKAEPLRAMLRSGICSALVTDADCARALLG"
     gene            complement(6240..7691)
                     /locus_tag="P5313_10170"
     CDS             complement(6240..7691)
                     /locus_tag="P5313_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRY80770.1"
                     /translation="MKSKTLSLTDIDSLFPADLSPDKKAQAKTLFLKKLSLEAHRFYG
                     GKLQTVPKCGIYGFNWFNVWYTPGVSKISTSIRDDNDVSFETTCRGNMVAVVSDSTRV
                     LGDGDCTSPGGLGVMEGKSLLMKYLGGVDAVPLCVDSRNEAGKHDPAKIIEFVKMCQH
                     SFGAINLEDISQPNCFRVLDDLRDACDIPVWHDDAQGTACVTLAGLINAVDLAGKKMR
                     DVKIVLLGAGASNTTIARLLMADGARGENIVMFDTKGSLHKGRKDIEASPENYRKWEL
                     CQSTNPKGILTMENAFRGADALVALSTPGPDVVKPEWVASMAPKAIVFACANPVPEIW
                     PHKAKEAGAFIVATGRGDFPNQVNNSICFPGILKGALAVRARKISDGMAIRCAHSLAD
                     FAKARGIHPDNIVPNMDESDVFSVEAADVAEQAVKEGLARVPKSREEVYAWAKKEIAA
                     ARKTFESMMERGDIPQPDEAMILAARDWALKQV"
     gene            complement(7722..8846)
                     /locus_tag="P5313_10175"
     CDS             complement(7722..8846)
                     /locus_tag="P5313_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRY80771.1"
                     /translation="MPGFSTEGAVWWPFDITERFMVDVFKGYGVPAGDAEVCAEVLIT
                     ADKRGIDSHGVGRLKPIYIDRIKDGQINPVTKIDLIKEGPTTAVLDANNGMGQVVSKK
                     AMLMAIEKAKKYGMGMVVVRNSNHYGIAGYYTGLAADHGMIGFSGTNARPSIAPTFGV
                     ENMMGTNPLCFALPTDEPFHFNIDCATSISQRGKIEVYARQKKDIPPGWVIGADGTTK
                     VDSIAVLDDLVKGTAALTPVGGIGEEGGGHKGYGWAATVEILSSALQQGAFMKSLLGF
                     DNGKKVPYKLGHFFMAVNIEAFTELAAFKKTSGDICRALRNSKKAPGQKRIWTAGEPE
                     WDAWLERKDKGVPLDEVERVQFAQMKAELKLTQYKFPWES"
     gene            complement(8977..9423)
                     /locus_tag="P5313_10180"
     CDS             complement(8977..9423)
                     /locus_tag="P5313_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY80772.1"
                     /translation="MALIDLIREDVVRVPLAANTKEGIIRELVQTLAAAGKVSDSEAA
                     SRAVLDREAKGSTGLEEGVAVPHGKTLAVQSLAVAVGVAPRGVDFEALDGKPSSLFFL
                     ILAPPDQSGPHIEALAEIARLCRSKAFCRTLVSSRDAKEVVELFQD"
     gene            complement(9436..9924)
                     /locus_tag="P5313_10185"
     CDS             complement(9436..9924)
                     /locus_tag="P5313_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY80773.1"
                     /translation="MTKPIRRILAYVDGTEQSVTAAQYAIVLAKSLGAELFALYVVNT
                     RALSELVKAHIFLDSEQEEYHRDLDEDAERYLNHVRNLARSKGVAVTAERSSGAVHQE
                     ILAKVREHGIDLLVLGEVSQIRSRRDELYNETERAMRSVACSVLIAKNEDRIAELFEE
                     LV"
     gene            complement(9921..10559)
                     /locus_tag="P5313_10190"
     CDS             complement(9921..10559)
                     /locus_tag="P5313_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRY80774.1"
                     /translation="MRAVAPTKTSLLRLREELKFARLGHELLDQKRNILVVELLTLVD
                     QAADFEKKVEASLETAYGSLQEAALGMGKLRVSSAAAAVNLEPSVTLRQRKVMGVVLP
                     VVETSFQERPPHYSPLGTSVWMDKSVRDFQDALKLMGRFAELKASIMRLAAEVRKTIR
                     KVNALEKIAIPDLEESVHYIQGRLEENERDMFTLMKLVKNRLTLEEAEAAMP"
     gene            complement(10563..11984)
                     /locus_tag="P5313_10195"
     CDS             complement(10563..11984)
                     /locus_tag="P5313_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRY80775.1"
                     /translation="MPEQETRNEARRRLMSGREYVGADRIDGPLLVLRKTHPVGYREL
                     AECVDREGHTRLGMVLDTSDDAVVIQVFEGTQGLTLPGTKVRFRGRPLTLGVTEKMLG
                     RVFDGLGRPIDGGPAPLAQEEVDVNGRPINPTSREYPRDFIQTGISAIDGMNTLIRGQ
                     KLPIFSGNGLPHNELAAQIARQAKIRGGSNDFAVVFAAMGVKHDVARYFTRSFEETGV
                     LENVALFLSLADDPSIERIITPRTALTLAEHLAYRKGLHVLVILTDMSNYCESLREIS
                     TIRGEIPSRKGYPGYLYSDLAEIYERSGMIRGTPGSITQLPILTMPSDDISHPIPDLT
                     GYITEGQIVFEREMSGRGIYPPVAGLPSLSRLMKDGIGEGMTREDHPHLASQLFAAYS
                     YVKDVRNLASVIGEEELTPLDHQYLEFGEFFERKFVSQGKDEDRPIERTLELGWEALK
                     LLPSEELHRVTDEEIETYYLGEG"
     gene            complement(11977..>13040)
                     /locus_tag="P5313_10200"
     CDS             complement(11977..>13040)
                     /locus_tag="P5313_10200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRY80776.1"
                     /translation="FGTGKTMTQHAIAKWCDADIIVYIGCGERGNEMTDVLTEFPKLV
                     DPRTGRSLMERTILIANTSNMPVSAREASVYTGVTLAEYYRDQGYHVAVMADSTSRWA
                     EALRELSGRMEEMPAEEGFPAYLPTRIADFYERAGYMDTLSGREGSVSIIGAVSPPGG
                     DFSEPVTQHTRRFVRCFWGLDRQLANARHYPAISWLDSYSEYLDDVTPWWEEHAGASW
                     AGERREILELLQREVRLQQVVKLVGPDALPDSQRFVLEVCALFKNAFLQQNAFDDVDR
                     YSSVDKQLRMLSLILVYWRRGAEAIKRGVTLVKLKQMKVLSDIIKMKFNIRDEGAAEF
                     DKLQARLERSLDQLESIYA"
BASE COUNT         1948 a         4371 c         4446 g         2275 t
ORIGIN      
        1 cctacctctt caccgggacc tggcggaagc ccctggccct cgatttcgac gcccgggggt
       61 acggggcctt cgcgggggat tccgttggtg tctggttcca ggacgcccgg gccccccggc
      121 tggggttctg ggcggggact gcgggaagcg aggggatcct ggccgggggg gcggcggcgc
      181 tctccctcct tcccccggga ggcggcttcg aatcctggtt cgccccggaa gtccccgccc
      241 ccgcccgtct cctggccgcg gggatggttt gggccggata cggattcccc gcgggtaggg
      301 tgatcgcggc cggggccgta tccgaggagg atcggggcgg ccggggctgg gcggtccgcg
      361 cggaggggga gtgggggcgc cggggatttt gtgcgagcgg acggatttcc tccgcctccc
      421 cgggctggag gggcctggac ggggatgcgg cggaccgctg ggaactgagg accgacgcgt
      481 cctgggccgt gatgccgcgg ttccgactgg agggccgggc ccgcatcgga caggacccgg
      541 actgtggtct cgactgggat gccctggccc ggggttcctg gaacggcaag gtctggacct
      601 ggggggcgga gctgggggcc tgcgactccg cccggctccc tccggtccgg ctggatcccg
      661 ccgtatgggc cggatacgaa accaggacgg ttcgggcttc cgcccgggcg tcctgggttc
      721 gggagggcgc ggacctgacc cgcacggagg tctccgccgc cctggatctg gggattcccc
      781 gcaagccggg cctccggctg gagggagccc ggagatggac ggtggaagga ccgaactgga
      841 aggcctccgc gaccctggag gtcccgacgg cccggggcgc ctggaccgcc cgggcgggga
      901 cttcggattg ggcggaggac ggggaggtcg tgccctggga actcgccgtc gtcctgcgct
      961 cgcgattccc gtagccgggt cctgtttgtc ttgcgcaccc ggcgcccttg gagcgatact
     1021 cgagcagacc cgatcaacat cgcagtcgga ggcggtatgg gcgttccaga ggcgcgggaa
     1081 tatcttagga gatggaaccg ggacggggat gtcctggaat tcgaccgttc cagcgcgacc
     1141 gtggagctcg ccgcccgggc cctgggggtg gagccgggcc gcatcgccaa gaccctttcg
     1201 ttccggggcc gggagcggcc cctcctggtc ctggccgccg gggacgcccg gatcgacaac
     1261 aagaagttca aggcggcctt cggcctcaag gcgtccatgc tgtccccgga ggaggccctc
     1321 gagcggacgg ggcatccggt gggcgggatc tgccccttcg gcctggccac ggagctggac
     1381 gtctacctgg acgagtccct gcgacgcttc gaccacgtct ttcccgcctg cggaagcggc
     1441 aactccgccc tccgctgcac cctggcccag ttggaagaat attccgcctc gaaggcctgg
     1501 gtggacgtct gctcgggcta ggagcctgtc ggacttgtgc ggggtgtcca ataagtcggt
     1561 tacttattgg acaccccttg cagtttctaa cgttctcttg agaacgttag aaactgctac
     1621 cctaatacca gctgtccaaa aagctatccg agctttttag acaacccccc gattaacggg
     1681 ctcctcggga gtttgagtgg tagaagaatc gcgtacatgc gatttttcta ctatttaggc
     1741 gggcaagccc gacaaactcc aagggtaggg ggtcggcgtt tcttaacagg gtgctgaaaa
     1801 actcgaagct tgtcagcacc ctgttattcc cggacagcca ggagtaccgc ccgggcttcc
     1861 acggccctgc cttccccgac gggacccagg ccctccatgg tcttggcctt gaaggacacc
     1921 gcctccacgt ccaggtccag gagttccgcg aggctggtcc ggatcgcctc cctgtggggg
     1981 gctagcttcg ggcgctccag gaccaccgtg cagtccaggt tgaccggccg ccagccggcc
     2041 cttcgaacca gatcccgggc gcggcgggcc aggtcccggg agtccgcgtc cttccactcc
     2101 gggtcacccg gcgggaagtg gatcccgatg tcccccaggg cggcggagcc caggagtgcg
     2161 tcgatcacgg cgtgcaggag gacgtccccg tccgagtatc cctcctcccc gcggtcggag
     2221 ggcacctcga cccctccgag caggaggcgc cgtccgggag ccaggcggtg gatgtcccag
     2281 ccttcgccga cccggaaggg ggccgccgcg ggctcgaccg gggtttcggg gcggaactcg
     2341 agatcctccc gaaaggtgat cttccggttg ctccgctccc cctccgtcca ggccacgggg
     2401 ccgacgtagc gggcccagag ttcggcgtcg tccgtggcct ccatcccctc cgcgtccgcc
     2461 cttcggtggg cctccagcag gggccccagc cggaagccct ggggggtctg ggcgttcgcc
     2521 agggcggccc gggggggatg gcgcaggacc aggccgtccg gggagatctc cttgggggtc
     2581 tccacgagac cggtcacggg tacgcaggcc ccgaccctgc ccgcgatggc ggccacccgc
     2641 tcggccaaac cttggctgag ccaggggcgg gcgccgtcgt gcaccaggac caccatgtcc
     2701 cggacggctt ccggaagggc ggggaccgat cccgaggcgg cttccgccgg tgccagggcc
     2761 cgggccaggg cttccaaccc gagccggacg gaatcccttc gggtaggtcc gccggggacg
     2821 accacgaacc ggtcccccag ccgctccagg tccgacttgg tgcgggcgtg cagggccgta
     2881 agaagttcgg acttcctctc ccggggggcg gtgatgacca gggaggaaag gccggtcagc
     2941 cggctgaagg cggagaccgc ccgatccagc acggccctgc cgcccagatc ctcggtttcc
     3001 ttcttgccgc cgaaccgctc tccacggccg gcggcggtga tgaggacggc taccggtccg
     3061 gtcatgatcg gaccgacccg gtccggccgg cggggcgctt ttccccctga accaggaggt
     3121 ctcgtacggc gtcctcgatg cgctccgggg tcggcacggc gttccgctcc aggttcgggc
     3181 tgtagggtac ggggatgtcg aagcccgcca cgcgccggat cggcgccttc agcaggggga
     3241 aggcctcgct atccgccacc agggctgcga tctccccccc gaagcctccc gtccgtacgg
     3301 cctcgtgtac gatgagcagg cgtccggtct tggcgacgct ggaaacgatg gcccgggtgt
     3361 ccagggggac cagggtccgg ggatccagca cctccacgga gactccctcg gactccagcc
     3421 gttccgccgc ggccaggcac atcccgacca tgcgtcccca ggccacgatg gtcaggtccg
     3481 agccgggccg cttcacgtcg gccttcccga agggcaccag gtactcttcc tcgggtacgg
     3541 ggccggtctc cttgtacagg agcttctgct ccaggaagac cacggggttg tcgtcccgca
     3601 cggccgtctt caggagaccc ttcgcgtcgt agggagtcga gggcacgacc accttgagcc
     3661 ccgggaagtg gcagaagacc gcctcctggc tctgggagtg ctgggccccg gccccggtgc
     3721 ccgagcccgc ggggctccgc accaccaggg gtacccggac cgctccgccg aacatgtacc
     3781 gcatcttggc ggcctggttc ccgatctggt ccagggccag ggagaggaaa tcgctgaagg
     3841 ggatctccac gatcggacgg gtgcccgtca gggccgcccc cgcggcggct cccgccagga
     3901 cggcctcgga gatcggggtg tccaggatac ggtccggtcc gagttcctcg atgagacccg
     3961 cggacacgcc gaaggtgccc ccgtagatcc ccacgtcctc ccccatgagg aagacccggg
     4021 gatccctgcg cagttcctcc ctcagggctt cccggacggc ctgggcgtag ctgatttccc
     4081 ggggtctccg ggctccggtc cccgcgtcag tccgcataga cgtccctcag gagatcctcc
     4141 aggccgggct cctcgcaggc ccgggcgtac cggaacgcgg cgtccacctc ccggcggacc
     4201 tcctcctcga tgtcctcaat ccggtcctcg gagaaggaac cctcctcgtt gagacgttcc
     4261 cggaaccggg ggatgggatc cctccgcctc cacgacgcga tctcctcctc ggtccggtac
     4321 acgttggcgt cgctccggga atgccccagg aagcggtagg tctgggccac caggaggagg
     4381 gggccgttcg ttttgacgta ctccttggcc tcccggaccg cctcgtagac ggccaggacg
     4441 tccatgccgt ccaccttcct gccgggaatt ccgtaggcgg ccgcccggag gctcaggtcc
     4501 tggacgttcg tggaacgccg tacgtgggtg gagaccccgt actggttgtt ctcgcagagg
     4561 aagaggaccg gcagcttcca gacggaggcc aggttcacgg actcgtggaa ggtcccctcg
     4621 ttcatggccc cgtccccgaa gaaacagacc gcgacccggt cctggcccag gcggcggatc
     4681 gagagggccg cccccacggc gatggggagg cccccgccca cgatgcccgt gccccccagg
     4741 ttcccccggg cgggatcggc caggtgcatg gagccgccct tgcccccgca gaccccggcc
     4801 cgcttgccga acatctccgc agccaagccg gaaagggccg tgcccttggc gaccgcgtgg
     4861 ccgtggcagc ggtgggtgct ggttatgtaa tccaggtccg agaggtttgc cgcggcgccg
     4921 gcggctatcg cttcctcgcc gacgtacagg tgcgtggtac cgtggatgtg tccccgggag
     4981 atatggtagt gtatctcctc ctcgaaatac cggatccgga gcatcctgcg gtacaggtct
     5041 tcgagcaggg aatcgggata tcccatgcgg gtgcgcgcgg cctgggccgc ggcggcagcc
     5101 gcgacggttg tcccgggcga tcggtctatc atgaaggctt cctcgcgcag agcgcctgaa
     5161 aaaatttgca gagcttgaaa aaaaatccgg ggtcaggata gtcttgagcc cgaaggcttg
     5221 tcaaggagag tccgggacgg ggggaagcta cggatgggcg aagacgcggg acggcgaacc
     5281 gaagactcca tccaggcggc cgacggcagg ccggacggat cccacagtct gctggccaag
     5341 atcgctcacc tttactatat agaaaacctc agccagaagg atatctccca gcggacccgg
     5401 atgtccgtgg ccagcgtatc ccgggccttg gcccgggccc gggagctcaa gatcgtctcc
     5461 atatcgatac agggggggga gaacgatttt tcccgcctgg aaatagagat cgagagaacc
     5521 ttcgatctcc cggaatgtct gctggtgccc agttacggcc ggatcgagca tacctaccag
     5581 ggcttggcgg ccgccgcggc ggatctcctg tcccgcaccc tgaaacccgg ggccgtcctg
     5641 ggggtcagct ggggggacac cctgaggtcc gtcggggaga gcctgccccg gatgccctcg
     5701 gtgcgggcgg acgtgatccc catcatcggc gccctgggct ggatcgagac cgggatctac
     5761 cccaacagca tcgcccgcac cttcgcccag aaactgggcg gtagttccta cctcgtgaac
     5821 acccccgcct tcgtggacga tccggaaacc cggaaagccc tgatggagga cagcaatttc
     5881 cgcccggtcc gggacatctg gagccgcctg gacatggcct tcctgggggt cagcgccctg
     5941 accgtggaca gctccatgta ccgcagcggc atcttcacgg aacccgacct ggaggggatg
     6001 cggaccgcgg gcggggaatg catcagcaac ttcgtggtct tcgacggccg tggacgtatg
     6061 gttcccacct ccgtgtcgga ccgcatggtc taccttcccc tggaggatct ccggagggtc
     6121 cgcaccgtgg cgatcgcggc ggcgggggcg gccaaggcgg agcccctccg ggccatgctc
     6181 cggagcggga tctgctccgc cctggtcacg gacgcggact gcgcccgggc cctgctcggc
     6241 tagacctgct tgagcgccca gtcccgggcg gccaggatca tggcctcgtc cggctggggg
     6301 atgtcccccc gctccatcat ggactcgaag gtcttccggg ccgcggcgat ctccttcttg
     6361 gcccaggcgt agacctcctc ccgggacttg ggaacccggg ccaggccctc cttcaccgcc
     6421 tgctccgcca cgtccgcggc ctccacggag aacacgtcgc tttcgtccat gttcggtacg
     6481 atgttgtccg ggtggatccc ccgggccttg gcgaagtccg ccagggagtg ggcgcagcgg
     6541 atggccattc cgtcggagat cttccgggcc cggaccgcca gggcgccctt caggatcccg
     6601 gggaagcaga tggagttgtt gacctggttg gggaagtccc cccggccggt ggccacgatg
     6661 aaggcccccg cctccttggc cttgtggggc cagatctccg gcaccgggtt ggcgcaggcg
     6721 aagacgatgg ccttgggggc catggaggct acccactcgg gcttgaccac gtccgggccc
     6781 ggggtggaca gggcgaccag ggcgtcggcg ccccggaatg cgttctccat ggtcaggatg
     6841 cccttggggt tcgtggactg gcagagctcc cacttgcggt agttctcggg gctggcctcg
     6901 atgtccttcc gccccttgtg gaggctgccc ttggtgtcga acatcacgat gttctcgccc
     6961 cgggccccgt cggccatgag gagccgggcg atggtggtgt tggacgcccc ggcgcccagg
     7021 aggacgatct tgacgtcccg catcttcttg cccgccaggt cgaccgcgtt gatcaggccc
     7081 gccagggtga cgcaggcggt gccctgggcg tcgtcgtgcc agaccgggat gtcgcaggcg
     7141 tcccgcaggt cgtccaggac ccggaagcag ttgggctggc tgatgtcctc caggttgatc
     7201 gccccgaagg agtgctggca catcttcacg aactcgatga tcttggcggg gtcgtgcttg
     7261 cccgcttcgt tccgggagtc cacgcagagg ggcacggcgt ccacgccgcc caggtacttc
     7321 atgaggaggg acttgccctc catgaccccg aggccgcccg gggaggtgca gtccccgtcc
     7381 cccaggaccc gggtggagtc gctgaccacc gccaccatat tgccccggca ggtggtctcg
     7441 aaggacacgt cgttgtcgtc ccggatggag gtcgagatct tggacacccc cggggtgtac
     7501 cagacgttga accagttgaa gccgtagatc ccgcacttgg gcacggtctg cagcttgccg
     7561 ccgtagaagc ggtgggcctc cagggaaagc ttcttcagga agagggtctt ggcctgggct
     7621 ttcttgtccg gggacaggtc cgcgggaaac aggctgtcga tgtcggtcag ggagagggtc
     7681 ttggatttca tggcttcctc cacggttgct tcacgttcgg cttaggattc ccacgggaac
     7741 ttgtactggg tcagcttgag ctccgccttc atctgggcga attggacccg ctccacctcg
     7801 tccaggggca ctcccttgtc cttacgctcc agccaggcgt cccactcggg ctccccggcg
     7861 gtccagatcc gtttctggcc cggggccttc ttggaattcc gcagggcccg gcagatgtcc
     7921 cccgaggttt tcttgaaggc cgcaagctcc gtgaaggcct cgatgttcac ggccatgaag
     7981 aagtgtccca gcttgtaggg caccttcttc ccgttgtcga agcccaggag gctcttcatg
     8041 aaggccccct gctggagggc ggaggagagg atctccacgg tcgcggccca gccgtagccc
     8101 ttgtgtccgc cgccttcctc cccgatgcct cccacggggg tcagagccgc ggttcccttg
     8161 accaggtcat ccagcacggc gatggagtcg accttggtcg tcccgtccgc cccgatgacc
     8221 cagcccggag gaatgtcctt cttctgccgg gcgtagacct cgatcttgcc ccgctgggag
     8281 atggaggtgg cgcagtcgat gttgaagtgg aagggctcgt ccgtgggcag ggcgaagcag
     8341 agggggttgg tgcccatcat gttctccacc ccgaaggtgg gggcgatgga cggccgggcg
     8401 ttagtgcccg agaagccgat catcccgtgg tccgcggcca ggcccgtgta gtaccccgcg
     8461 atcccgtaat ggttggagtt acggaccacc accatgccca tcccgtactt tttggccttc
     8521 tcgatggcca tgagcatggc cttcttggat accacctgcc ccatgccgtt gttggcgtcc
     8581 aggaccgcgg tggtggggcc ttccttgatc aggtctatct tggtgacggg attgatctgc
     8641 ccgtccttga tccggtcgat gtagatgggc ttcaggcgtc cgaccccgtg ggagtcgatc
     8701 ccccgcttgt ccgcggtgat cagaacctcc gcgcagacct cggcgtcccc ggcgggaacc
     8761 ccgtacccct tgaacacgtc caccatgaaa cgctccgtga tgtcgaaggg ccaccagacg
     8821 gcgccttcgg tcgagaaacc aggcattcga tcctccttta tcttattcta cagggcatga
     8881 tacgccgctt cgcggtcttt ggctagattt ttcggttccg gaacccgccg cgtcctgcac
     8941 ggcaggaggc gtgcggacga cccgcggctc tccgggtcag tcctggaaga gctccaccac
     9001 ttccttggcg tcccgggatg agacgagggt ccggcagaag gccttggacc ggcacagccg
     9061 ggcgatctcc gccagggcct cgatgtgggg tcccgactgg tccggggggg ccaggatgag
     9121 gaagaagagg ctggagggct tcccgtccag ggcctcgaag tccacccccc ggggcgccac
     9181 gcccacggcg acggccaggc tctgcaccgc gagggtcttg ccgtggggaa ccgccacccc
     9241 ctcctccagt cccgtactgc ccttggcctc ccggtccagc acggcccggg aagccgcttc
     9301 ggaatcggac accttgccgg ccgcggccag ggtctggacc agttcgcgga tgatcccctc
     9361 cttggtattc gccgctaggg gaacccggac gacatcctcc cggatcaggt cgatcagcgc
     9421 catatcaaac ccccgtcaca cgagctcttc gaagagctcg gcgatgcgat cttcgttctt
     9481 ggctatgagg acggagcagg cgacgctgcg catggcccgc tcggtctcgt tgtacagctc
     9541 gtcccggcgg ctccggatct gggagacctc gcccaggacc aacaggtcga tcccgtgctc
     9601 ccgtaccttg gccaggatct cctggtggac cgccccgctg gaccgttccg cggtcacggc
     9661 gacgcccttg gagcgggcca ggttccggac atggttcagg taacgctccg cgtcctcgtc
     9721 caggtcccgg tggtactcct cctgctcgga atccaggaag atgtgggcct tcaccagctc
     9781 cgagagggcc cgggtgttca ccacgtacag ggcgaagagc tccgcgccca ggcttttggc
     9841 caggacgatg gcgtattgcg ccgcggtcac ggactgctcc gtgccgtcca cgtacgctaa
     9901 aatcctccga atgggttttg tcatggcatc gccgcctccg cctcttccag ggtcagccgg
     9961 ttcttcacca atttcatgag ggtgaacatg tcccgctcgt tctcctcgag ccgcccctgt
    10021 atataatgca ccgattcctc caggtcggga atggcgattt tctccagggc gttcactttc
    10081 cggatggtct tccggacctc cgcggccagg cgcatgatgg acgccttgag ctccgcgaag
    10141 cgccccatca gcttcagggc atcctggaag tcccggacgg acttgtccat ccagacgctg
    10201 gttcccaggg ggctgtagtg gggggggcgt tcctggaagg aagtctccac caccggcagg
    10261 accacgccca tcaccttgcg ctggcgcagg gtcaccgaag gctccaggtt caccgcggcg
    10321 gcggcggagg aaacgcgcag cttgcccatc cccagggcgg cctcctggag ggagccgtag
    10381 gccgtttcca gggaggcctc gaccttcttc tcgaaatccg cggcctggtc caccagggtc
    10441 aggagctcca ccaccaggat gttgcgtttc tggtccaata gctcgtgccc caatcgggcg
    10501 aacttcagct cctcccggag ccggaggagg ctggtcttgg tgggggctac ggcgcgcacg
    10561 gcctagccct cccccaggta gtacgtctcg atctcctcgt ccgtgacccg gtggagctcc
    10621 tccgagggca ggagcttcag ggcttcccag ccgagctcca gggtgcgctc gatgggccgg
    10681 tcctcgtcct tcccctggct gacgaacttg cgctcgaaga actccccgaa ttccaggtac
    10741 tggtggtcca ggggggtgag ctcctcctcc ccgatgaccg aggccaggtt ccgcacgtcc
    10801 tttacgtagc tgtacgccgc gaagagctgg ctggccaggt gggggtggtc ttcccgggtc
    10861 atgccctcgc cgatgccgtc cttcatgagc cgggacaggg agggcaggcc cgccacgggg
    10921 gggtagatcc cccgtccgga catctcccgc tcgaagacga tctggccctc ggtgatgtac
    10981 ccggtcaggt ccgggatggg gtgggagatg tcgtcgctgg gcatggttag gatgggcagc
    11041 tgggtgatgg agcctggggt gccccggatc atgccggacc gttcgtagat ctccgccaga
    11101 tccgagtaaa ggtagcccgg gtagcccttg cgggagggga tctccccccg gatggtggag
    11161 atctccctaa gggactcgca gtagttggac atgtccgtga ggatcaccag gacgtggagc
    11221 cccttccggt aggccaggtg ctccgccagg gtcagggccg tccggggggt gatgatccgc
    11281 tcgatggagg ggtcgtccgc cagggacagg aagagggcca cgttctccag gaccccggtc
    11341 tcctcgaagg accgggtgaa gtaccgggcc acgtcgtgct tgacccccat ggccgcgaag
    11401 accacggcga agtcgttcga gcctccccgg atcttggcct gccgggcgat ctgggcggcc
    11461 agctcgttgt ggggcagtcc gttgcccgag aagatcggca gcttctggcc ccggatcagg
    11521 gtgttcatcc cgtcgatcgc ggagattccg gtctggatga agtcccgggg gtattcgcgg
    11581 gaggtggggt tgatgggccg gccgttgacg tccacctcct cctgggccag gggggcggga
    11641 cccccgtcga tgggccgccc caggccgtcg aagacccggc ccagcatctt ctcggtgacc
    11701 cccagggtca gggggcggcc ccggaaccgg accttggtcc ccggcagggt caggccttgg
    11761 gtcccctcga agacctggat caccaccgcg tcgtcactgg tgtccaggac catgcccagc
    11821 cgggtatgcc cctcccggtc cacgcactcc gccagctccc ggtatcccac gggatgggtc
    11881 ttccgtagga cgaggagggg gccgtcgatg cggtccgcgc cgacgtattc ccgcccggac
    11941 atgagtctcc ggcgcgcctc gttccgggtt tcctgctcag gcatagatgg actccagttg
    12001 atccagggac cgttccagcc gggcctggag cttgtcgaac tccgcggctc cctcgtcccg
    12061 gatgttgaac ttcatcttga tgatgtccga caggaccttc atctgcttga gcttgaccag
    12121 ggtcacgccc cgcttgatcg cctcggcgcc gcgacgccag tagaccagga tcagggagag
    12181 catgcgcagt tgcttgtcca ccgaggaata ccggtccacg tcgtcgaagg cgttctgctg
    12241 gaggaaggcg ttcttgaaga gggcgcagac ctccaggaca aagcgctggg aatcgggcag
    12301 ggcgtcgggg cccaccagct tgaccacctg ctggagccgg acctcccgct ggaggagctc
    12361 caggatctcc cgccgctccc cggcccagga ggccccggcg tgctcctccc accagggggt
    12421 tacgtcgtcc aggtactccg agtagctgtc cagccaggag atggccgggt agtgccgggc
    12481 gttggccagc tgacggtcca ggccccagaa gcagcggacg aagcggcggg tgtgctgggt
    12541 cacgggctcc gagaagtcgc cgcccggggg gctcacggcc ccgatgatgg agaccgagcc
    12601 ctcccggccg gacagggtgt ccatatagcc ggcccgctcg tagaagtccg cgatccgcgt
    12661 gggcaggtag gccgggaaac cctcctccgc gggcatttcc tccatgcggc ccgagagctc
    12721 gcggagggcc tccgcccagc gggaggtgga gtccgccatg acggccacgt ggtagccctg
    12781 gtcccggtag tactcggcca gggtaacccc ggtgtacacg ctggcctccc gggcggacac
    12841 gggcatgttg gaggtgttcg cgatcaggat ggtccgctcc atcaagctcc ggcccgtccg
    12901 gggatccacc agcttgggga actccgttag gacgtcggtc atctcgttgc cccgctcccc
    12961 gcagccgatg tacacgatga tgtccgcgtc gcaccacttg gcgatcgcgt gctgggtcat
    13021 ggtcttgccg gtgccgaagc
//
LOCUS       DAOTEI010000097        13023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3341_length_13023_cov_4.733883, whole genome shotgun sequence.
ACCESSION   DAOTEI010000097 DAOTEI010000000
VERSION     DAOTEI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 13023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13023
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3341_length_13023_cov_4.733883"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..461
                     /gene="rplQ"
                     /locus_tag="P5313_10205"
     CDS             21..461
                     /gene="rplQ"
                     /locus_tag="P5313_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRY80777.1"
                     /translation="MNHKKGFNKLNRNASHRKALMRNMMTVLFKHERIVTTKAKALEV
                     RRFAEKEITRAKVDSVHNRRLAARKIMDEAVLVKLFQDIGVRMKDRPGGYTRVLKIGF
                     RKQDAAEMVVLELVDRKADDEKNAKKAAAKAKKAEKKAAAAKAE"
     gene            475..645
                     /locus_tag="P5313_10210"
     CDS             475..645
                     /locus_tag="P5313_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80778.1"
                     /translation="MSKAHRGKGLRDQVAGGRGTCPVCKREAVKVLYEQEIEGKKTKI
                     CKTCKATIANKK"
     gene            713..1618
                     /gene="rarD"
                     /locus_tag="P5313_10215"
     CDS             713..1618
                     /gene="rarD"
                     /locus_tag="P5313_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRY80779.1"
                     /translation="MEKPDRGPRSAEAQGILFGIAAFGMWGLFPLYWKSLSAVPAFQI
                     LAHRILWAFAFTLVLTFLLRRGRVLAEVFRSRNRLLSTAAAGLLISVNWGIYIWAVNA
                     SQILETSLGYYLNPLVSAALGALVLRERLDRGMVVSYVVAGLGIALQAFSYGRLPWIA
                     LSLAFTFALYGLVKKMASLDVLSGLTLETGVAFPFALAFLLLEERAGRGAFGNLGVLN
                     TTLLALAGPVTAVPLLSYAAGVVRLPLSKMGLLQYISPTLQLLVGVLVYGERLDLTRA
                     VTLGCVVAALVIFGATRGRRDASGG"
     gene            1684..2172
                     /locus_tag="P5313_10220"
     CDS             1684..2172
                     /locus_tag="P5313_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRY80780.1"
                     /translation="MVYKTSVAVLVLSSLLSGCATLGTPADVLEDLSWELLEYIPYDA
                     KETIAVYYFLEDRRESSRSDYVLNLLSTGMANAVHEEGRPLVLVSRTALDRILKEQEF
                     QVSALADSGTQLLLGKQLGAGIIVTGTITPVPDGYEVNAQLIRVETGAVLGGVVRTIY
                     PE"
     gene            2278..2757
                     /locus_tag="P5313_10225"
     CDS             2278..2757
                     /locus_tag="P5313_10225"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRY80781.1"
                     /translation="MKKSAFLAVFLFLLALSLWAAGGEATVQVKETILRDQPTFTGKT
                     LGILKYGAKVSILEEKSGWARVSAEGGKLQGWVHGSAITKKSVALSSQGGTGTGATSA
                     EVSLAGKGFSAEVEAQYRSQNAALDFATIDAMEKVRFRPDELKAFLSAGGILPKGGL"
     gene            2757..3677
                     /locus_tag="P5313_10230"
     CDS             2757..3677
                     /locus_tag="P5313_10230"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRY80782.1"
                     /translation="MRNRTYGSCLRTLALAAAASTALASCASIVEKTSGFAVASGLMT
                     QDQAASSAKLAQAVDKTFEDITPEQEYYIGRSVGAQVLSTSRPYSNAAVSRYVNLLGQ
                     SLALFSTRPETFGGYHFQVLDSDSINAFAAPGGHIFVTRGMLRLCSTEHELAAVLAHE
                     IAHVQFQHGLKAIKASRLSSVFTIIGTEAAKNLSPGKLQEVTAQFADSITDITSSLIN
                     SGYSQATEFEADKGAVAILQNAGYNPAALVSMLAAMKTRLKPGAPDFAKTHPDPETRI
                     DRVRRQLGTLAPVVESPQSRERRTRALAGV"
     gene            3683..5845
                     /locus_tag="P5313_10235"
     CDS             3683..5845
                     /locus_tag="P5313_10235"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY80783.1"
                     /translation="MRAASAALGRKRKAGHKGPGRRALEGALLGLLVAGVFAGLGSFG
                     ALDALENRLWDLRVRTFARPSEATDRIRLILLDQGSLDWAEKELGVPWPWPREFYGVI
                     VSFLTRSGARAAAFDVLYTETSFYGVEDDESFAAAVREGGRTVAAVQLSREQGKDLSW
                     PSGAAEPPAAALNGVSLADLLRGVFPIPELSAAAAALGNVLAVPDPDGVNRRLAPAAR
                     FDGRTVPTLALAAYCLGEGIRDLEFRPGAVKAGSRSIPLSGDGHVILRYRGPSQTHRA
                     VNAGAVLRSETALRDGGAPEIDPEFFSDKYVFFGFSAPGLKDLRPAPVGEAYPGVEVH
                     ATLLDNILAGDAVRPLPLWASAVLVLVLGMAAGTAIRLCRDALQSAAVLAVALALPFA
                     AAWIAAGAGIWAPAAAPEGAVLFALIGGFVVNYAGEGRQKRFIKEAFSQYLSPVVIER
                     LVHDPDKLTLGGEKRCLSILFSDIRGFTSISEGLDPVSLTALLNDYLSEVTEIIYRFG
                     GTIDKYEGDAVIAFWNAPLDLPTHALNSVKAALEYQKRLEEIRPRMAETAGRDVYARI
                     GINTGDVVIGNMGSRQRFNYTFLGDAGNLASRLEGINKQFGTFVMVSEFTKAAAGEDP
                     EVSYRELSRVTVVGKSEPVRVFEPLYRDEYERRAEVLDRFGRGLGLYYAGDFRNALGE
                     FEAIRDRDAPAAAYCERVGRLLADPPREWDGVWAITEK"
     gene            5850..7397
                     /locus_tag="P5313_10240"
     CDS             5850..7397
                     /locus_tag="P5313_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HRY80784.1"
                     /translation="MTGVKGFDNELYIREQASFIRERMRRFEGRLYLEFGGKLLSDLH
                     AARVLPGYDPNVKVRLLKELREDAEILLCIHAGAIEHRKIRADYGITYDADALRLIDD
                     LAEWGLSVSAVVITRWEGQAAARAFRTKLENRGLTVYAHAPTKGYPTDVDLIVSEEGY
                     GANPRIATTKPLVVVTGPGPGSGKMATCLSQVYHEHRAGGKAMYAKFETFPIWNLPLK
                     HPVNIAYEAATLDLKDFNLIDPFHLEAAGEASVNYNRDVESFPILRAILERITDGDAA
                     YRSPTEMGVNRAGFAIVDDEACREAAKQEVVRRYLRCASEYMMGMADKASLEKADLLC
                     KELGIGPEYRPVVPAARRAAEEAATVAESTVPGAAAPHGVDSAFCGAALQLPGGEIVT
                     GKNSRLLHAATAAVLNGVKRLAGIPDSIPLLSPLVIESIEKLKSEILRGRNPSLDVDE
                     ALIALSIGAALTPMVQSCLDQLHRLRGCQMHLSHVPSPGDEAGLRKLGIELTSDPVYA
                     SKRLFVE"
     gene            7510..8715
                     /gene="trpB"
                     /locus_tag="P5313_10245"
     CDS             7510..8715
                     /gene="trpB"
                     /locus_tag="P5313_10245"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRY80785.1"
                     /translation="MSDYFKTYPDAKGNYGQYGGSFIPPNLQAEMEKITDAYYSISKS
                     HEFISELRSIRKHFQGRPTPVYFAKRLSDEYGGRIYLKREDLNHTGAHKLNHCMGEAL
                     LAKYLGKKKLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIAKEHPNVVRMKILGA
                     KVVPVSRGLRTLKEAVDSAFEAYMADPVTAIYCIGSVVGPHPFPMMVREFQRVVGIEA
                     KEQFFEMTGEHPDSVMACVGGGSNAMGIFSAYIEDQNVKLYGVEPMGTGTKTGEHAAT
                     ITYGKPGMIHGFKCYLLQDEQGQPSPVYSIASGLDYPGVGPEHSMLHDLGRVTYTSVT
                     DAEAVDAFFQLSRKEGIIPALESAHAVAYALKLARREPRRSILVNLSGRGDKDIDFVV
                     EKHGEQYGI"
     gene            complement(8778..9245)
                     /locus_tag="P5313_10250"
     CDS             complement(8778..9245)
                     /locus_tag="P5313_10250"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80786.1"
                     /translation="MKIKQISVVLENTAGQLYALSQVLHKGDIDIRSIFVSEETEFSS
                     VHLIVDKPDKAVGLLRERGYFLKEVEVFALKAEDRPGGLMKILEILKANNLNIEYVYG
                     FGEKAENKAVFIFRITDIDKAIEVLKTGVMEFLHAGHVEGTRKSSAWELIENL"
     gene            complement(9282..10583)
                     /locus_tag="P5313_10255"
     CDS             complement(9282..10583)
                     /locus_tag="P5313_10255"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRY80787.1"
                     /translation="MSDRLVPNLGKCSLQSPIKLSNTVGDGIANYIRDEDLVRHDVHV
                     RAGEVTDLTQFFEKAGPREKLYFAPQHVHAAIVTCGGLCPGLNDVIRSVVRCLWYRYG
                     VRRISGVRFGYRGLIPDYGYDIRPLNPDVVDDIHKLGGTVLGSSRGGGDRTSDIVDSL
                     ERLNVNILFTIGGDGTQKGSLAIAEEIERRNLKVAVVGIPKTIDNDLLFVDRSFGFDT
                     AVAQASHAVTAAHTEAHSSINGIGLVKLMGRESGFIAAHTVLAVHEVNFVLVPEVDFD
                     LEGPNGLFAHLEKRLAERNHAVIVAAEGAGQKYSAAEGTDASGNRKLGDIGIFLKDAI
                     NAHFKKKGIEVNLKYIDPSYIIRSSPASPVDSVYCDRLGSNAVHAAMAGKTRILIGMV
                     NNEFVHIPTGVAIAQRRKVDPESSLWRDAIEATRQPISMVN"
     gene            complement(10654..11541)
                     /locus_tag="P5313_10260"
     CDS             complement(10654..11541)
                     /locus_tag="P5313_10260"
                     /inference="COORDINATES: protein motif:HMM:NF009888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HRY80788.1"
                     /translation="MLDYQLVQAFAAVLDEGGFARAADRLCITQSAVSQRVKQLEDQI
                     GRALVIRDTPPRPTEAGARLLRHYRQVLALEGETVDAVSAKSPACRPHVPVAVNADSL
                     FVWFLEAAAAFARSSGTTLELFVDGHEQTLRYLRSGAVAGCVTSERDAVEGCTATRIG
                     SLRYVLVASGEFSRRWFPEGFSREAATRAPLVNLDRNDRFQLRVLYKAFGDPQVTPPA
                     HYVPVADAYFEAIRAGLGYGLVAKSKVLPGLADGTLVDLDPGLRTEIPLYWHVWKYQS
                     DTLRDLSEVVVREGGRLLA"
     gene            11667..>13023
                     /locus_tag="P5313_10265"
     CDS             11667..>13023
                     /locus_tag="P5313_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRY80789.1"
                     /translation="MRLTFDELPPDPVFVPGIRRAPRRDAPLTPAQEILALKNALRYV
                     PERWHEKLAPEFLEELRTRGRIYGYRFRPSGPLKGKPIDSYSGRCVEGKAFQVMIDNN
                     LDFDVALYPYELVTYGETGQVCQNWMQYRLIKKYLEVLDENSTLVVESGHPLGLFRSH
                     PLAPRAMITNGLMIGMFDNSKDFAVAAALGVANYGQMTAGGWMYIGPQGIVHGTFNTL
                     LNAGRLKLGIPADKDLAGRLFVSSGLGGMSGAQGKAAVIAGAACVIAEVDPSRVETRL
                     SQGWVDAVEETPEAAFRRAEEARTAGKPIAVAYLGNVVDLLEWAIAKNVKIDLLSDQT
                     SCHAAYEGGYCPAGLSFPERTRMLKEDPARFRTLVDGSLRRHFEAIRTLAARGTYFFD
                     YGNSFLKAVWDAGVKEVSRNGTDEKDGFIFPSYVEDILGPELFDYGYGPFRWVCLSGK
                     PE"
BASE COUNT         2172 a         4404 c         4307 g         2140 t
ORIGIN      
        1 catgcagaag gaagagccga atgaaccata agaaaggctt caacaagctg aatcggaacg
       61 cctcccaccg caaggccctg atgcggaaca tgatgacggt cctgttcaag cacgagagga
      121 tcgtcaccac caaggccaag gccctggaag tccgccgctt cgcggagaag gagatcaccc
      181 gggccaaggt tgactccgtc cacaaccgcc gcctggcggc ccgcaagatc atggacgagg
      241 ccgtcctggt caagctcttc caggacatcg gcgtccgcat gaaggatcgg ccgggcggat
      301 acacccgggt cctgaagatc gggttccgca agcaggacgc cgcggagatg gtggtcctcg
      361 aactcgtgga ccgcaaggcc gacgacgaga agaacgccaa gaaggcggcc gccaaggcta
      421 agaaggccga gaagaaggcc gccgccgcca aggccgagta ggggaggaag gattatgtcc
      481 aaggctcatc gcgggaaggg actgcgtgac caggtcgcgg gcggccgggg cacctgcccg
      541 gtctgcaagc gggaggccgt gaaggtcctc tacgagcagg agatcgaggg caagaagacc
      601 aagatctgca agacctgcaa ggcgaccatc gccaacaaga agtagccccc gggctacccc
      661 cagagcggcc gcccccgggc ggccgtttcg tttttcattt caaggagacg tcatggaaaa
      721 gccggaccgg ggtccccgtt ccgccgaggc ccagggtatc ctcttcggga tcgcagcctt
      781 cgggatgtgg gggctcttcc ccctgtactg gaagagcctc tccgccgtcc ccgccttcca
      841 gatcctggcg caccgaatcc tctgggcctt cgccttcacc ctggtcctga ccttcctcct
      901 gcggcggggc agggtcctgg cggaggtttt ccgttcccgg aaccggctcc tgtccacggc
      961 ggccgcgggg cttctgatca gcgtcaactg ggggatctac atctgggcgg tcaacgcctc
     1021 ccagatcctg gaaacctctc tgggctacta cctgaatccc ctggtttccg cggccctggg
     1081 cgccctggtc ctgcgggagc gcctggaccg ggggatggtg gtctcctatg tcgtcgcggg
     1141 cctgggcatc gccctgcagg ctttttccta cggccgcctc ccctggatcg ccctttccct
     1201 ggccttcacc ttcgccctct acggcctggt gaagaagatg gccagcctgg acgtcctgtc
     1261 gggactgacc ctggagaccg gagttgcctt ccccttcgcc ctggccttcc tattgttgga
     1321 ggagagggcg ggccgggggg ccttcgggaa cctgggcgtc ctgaacacca ccctcctggc
     1381 cctggcgggg cccgtgaccg ccgtgcccct tttgtcctac gccgccgggg tggtccggct
     1441 gcccctgtcc aagatgggcc ttctacaata catatccccg accctccagc tcctggtcgg
     1501 ggtcctggtg tacggggaga ggctggacct gacccgggcc gtcaccttgg gctgcgtggt
     1561 ggccgccctg gtgatcttcg gggcgacccg cggccggagg gacgcttccg ggggctgacc
     1621 ggccgagccg cgcttgcggt tcggaggata tggagtatac ttggaggcaa gaggatcccg
     1681 cgcatggttt acaagactag cgttgcggtt cttgtgttgt cttccctcct gtccggctgc
     1741 gccaccctgg gcaccccagc ggacgtcctt gaggacctgt cctgggaact cctggagtac
     1801 attccctacg acgcgaagga aaccatcgcg gtctactatt tcctcgagga tcgccgggag
     1861 agctcccgga gcgactacgt cctgaacctc ttgagtacgg ggatggcgaa cgccgtccac
     1921 gaggagggtc gtcctctggt gctggtcagc cggacggccc tggaccgcat ccttaaggaa
     1981 caggagttcc aggtctccgc cttggccgat tccggcaccc agctccttct ggggaagcag
     2041 ttgggcgcgg gaatcatcgt caccgggacg atcactccgg tcccggacgg ctacgaggtg
     2101 aacgcccagc tgatccgggt cgaaaccggc gcagtcctgg gcggggtggt acggaccatc
     2161 tatccggaat agcgcccgcc gcccggacat gcctccttgt tggacggatt gcgattccca
     2221 tccttcgtgt ccatgctata ctcccgggaa atcctaatcc tgtggagtgt ccatccgatg
     2281 aagaagagcg cattccttgc ggtattcctg ttcctcctgg ccctgtccct ctgggcggcg
     2341 ggcggggagg cgaccgtcca ggtcaaggag accatccttc gggatcagcc gactttcacc
     2401 ggaaaaaccc tcggcatact caagtacgga gccaaggtct ctatcctgga ggagaaatcg
     2461 ggctgggccc gggtttccgc ggagggcggg aagctgcaag gctgggtcca cggatccgcg
     2521 atcaccaaga aatccgtggc cctgagctcc cagggcggca ccgggaccgg agccacgagc
     2581 gccgaggtct ccctggccgg aaagggattc agcgccgagg tggaagccca gtaccgcagc
     2641 cagaacgcgg cccttgattt cgcgaccatc gacgccatgg aaaaggtgcg tttccggccc
     2701 gacgagctga aggccttcct gtcggccggc ggcatcctcc cgaagggagg cctgtgatga
     2761 ggaaccgaac gtacgggtcg tgccttcgga ccctcgcgct ggccgcggcg gcttcgacgg
     2821 ccctggcttc ctgcgcttcc atcgtcgaga agacttcggg cttcgcggtc gcttcgggcc
     2881 tgatgaccca ggaccaggcc gcctcctccg ccaagctggc ccaggcggtg gacaagacct
     2941 tcgaggacat caccccggaa caggagtact acatcggccg gtccgtgggg gcccaggtcc
     3001 tgagcacgtc ccggccctac agcaacgcgg ccgtctcccg gtacgtgaac ctgctcggcc
     3061 agtccctggc cctcttctcc acccgccccg agactttcgg gggctatcat ttccaggtcc
     3121 tggacagcga ctcgatcaac gccttcgccg ccccgggagg ccacatattc gtgacccggg
     3181 ggatgctgcg cctatgctcc acggaacacg agctcgccgc ggtcctggcc cacgagatcg
     3241 cccacgtgca gttccagcat ggcctgaagg ccatcaaggc gagccgcttg agctcggtct
     3301 tcacgatcat cggaaccgag gccgccaaga acctgagccc ggggaaactc caggaggtga
     3361 cggctcagtt cgcggactcc atcaccgata tcacgagctc tctgatcaat tccggatact
     3421 cccaggcgac ggagttcgag gcggacaagg gagccgtggc gatactccag aacgccggat
     3481 acaatcccgc cgccctggtg tccatgctgg cggccatgaa gacccggctc aagcccggcg
     3541 ccccggactt cgccaagacc catccggatc cggagacccg gatcgaccgg gtccggcgcc
     3601 agctgggcac cctggcgccc gtggtcgaga gcccccagtc ccgggaacgc cggacccggg
     3661 ccctggcggg ggtttgaccc gcatgcgcgc cgcgtcggcg gccctgggca ggaagcgcaa
     3721 ggccggccac aagggccccg ggagacgggc cctggaaggg gccctcctcg ggctcctggt
     3781 cgccggggtc ttcgccgggc tgggctcttt cggcgccctg gatgccctgg agaaccggct
     3841 ttgggacctg cgggtccgca ccttcgcccg gccctcggag gcaaccgacc ggatccgcct
     3901 gatcctcctg gaccagggaa gcttggactg ggcggaaaag gagctggggg tcccctggcc
     3961 ctggccccgg gagttctacg gggtcatcgt atccttcctg acccggtccg gagcccgggc
     4021 cgcggccttc gacgtcctgt acaccgaaac ctccttctac ggggtcgagg acgacgagtc
     4081 cttcgccgcg gcggtccggg agggcggccg cacggttgcg gcggtccagt tgagccgtga
     4141 acagggcaag gacctctcct ggccgtccgg ggccgccgag ccccccgcgg cggccctgaa
     4201 cggggtatcc ctcgcggatc ttctccgggg cgtcttcccc ataccggaac tgagcgccgc
     4261 ggcggccgcc ctgggaaacg tcctggcggt gccggatccg gacggggtca accggaggct
     4321 ggcaccggcc gcccgtttcg acggccggac cgtaccgacc ctggccctgg cggcctactg
     4381 cctcggggag gggatccggg acctggagtt ccgccccggt gccgtgaaag ccgggagccg
     4441 gagcatcccg ctttccgggg acgggcatgt catcctccgg taccgggggc ccagccagac
     4501 ccaccgggcg gtcaacgcgg gggcggtcct ccggtccgag acggccctcc gggacggagg
     4561 cgcgccggag atcgatccgg agttcttctc ggacaagtac gtgttcttcg ggttcagcgc
     4621 cccggggctc aaggacctgc gccccgcccc ggtgggggag gcgtatcccg gagtggaggt
     4681 ccacgcgacc ctcctggaca acatcctggc cggcgacgcg gtccggccgc ttcctctttg
     4741 ggcctccgcg gtccttgttc tggtcctggg catggccgcg gggacggcca tccggctctg
     4801 ccgggacgcc ctccagtccg ccgcggtcct ggccgtagcc ctggccctgc ccttcgccgc
     4861 cgcctggatc gccgcgggtg ccgggatctg ggcgcccgcg gccgccccgg agggggccgt
     4921 cctcttcgcc ttgatcggcg gattcgtggt gaactacgcc ggcgagggcc gccagaagcg
     4981 cttcatcaag gaagccttca gccagtacct ctccccggtg gtcatcgagc ggctggtcca
     5041 tgaccccgac aagctgaccc tgggcgggga gaaacgctgt ctgtccatcc tgttcagcga
     5101 catccggggc ttcacgagca tctccgaggg gctggacccc gtctccctca cggccctcct
     5161 gaacgactac ctttccgagg tgaccgagat catctaccgc ttcgggggga ccatcgacaa
     5221 gtacgaggga gacgccgtca tcgccttctg gaacgccccc ctggacctgc cgacccatgc
     5281 cctcaattcc gtcaaggccg ccctggagta ccagaagcgg ctcgaggaaa tacgcccccg
     5341 catggccgag accgcgggcc gggacgtcta tgcccgcatc gggatcaaca ccggggacgt
     5401 agtcatcggg aacatgggct cccggcagcg cttcaactac accttcctgg gggacgcggg
     5461 caacctggcc tcccggctgg aggggatcaa caagcagttc ggtaccttcg tgatggtctc
     5521 cgagttcacc aaggccgccg cgggggagga tcccgaggtc tcgtaccggg agctctcccg
     5581 ggtgaccgtg gtgggcaaga gcgagcccgt ccgggtcttt gagcctttgt accgggacga
     5641 gtacgagcgc cgcgcggagg tcctcgatcg gttcgggcgc ggcctggggc tgtactacgc
     5701 gggagacttc cggaacgcct taggggagtt cgaggcgatc cgggatcggg acgcccccgc
     5761 ggcggcctac tgcgagaggg tgggacggct ccttgcggac ccgccccggg agtgggacgg
     5821 agtatgggcg ataacggaga agtgacggga tgacgggcgt caagggtttc gacaacgagc
     5881 tgtacatacg ggaacaggct tccttcatcc gggagcggat gcgccgcttc gaagggcggc
     5941 tctacctcga gttcggggga aagctccttt cggacctgca cgcggcccgg gtcctgcccg
     6001 gctacgatcc caacgtcaag gtacgcctgc tcaaggagct ccgggaggac gcggagatcc
     6061 tcttgtgcat ccacgcgggg gccatcgagc accgcaagat ccgggcggat tacgggatca
     6121 cctacgacgc ggacgccctg cgcctgatcg acgacctggc cgagtggggg ctgtccgtct
     6181 ccgcggtggt gatcacacgc tgggagggcc aggccgcggc ccgggccttc cggaccaagc
     6241 tggagaaccg gggcctcacg gtctacgccc acgcccccac caagggctac ccgacggacg
     6301 tggacctcat cgtctccgag gagggctacg gcgccaatcc ccggatagcg acgaccaagc
     6361 ccctggtggt cgtgaccggc ccggggccgg gctcgggcaa gatggccacc tgcctgtccc
     6421 aggtctacca cgagcaccgg gccggcggaa aggccatgta cgccaagttc gagaccttcc
     6481 cgatctggaa cctccccctg aaacaccccg tgaacatcgc ctacgaggcc gccacgttgg
     6541 acctcaagga cttcaacctc atcgacccct tccacctgga ggccgcgggc gaggcttcgg
     6601 tgaactacaa ccgggacgtg gagtccttcc ccatcctccg ggccatcctg gagcgcatca
     6661 ccgacgggga cgcggcctac cgttccccca cggagatggg ggtcaaccgc gccggcttcg
     6721 ccatcgtcga cgacgaggcc tgccgggagg ccgccaagca ggaggtggtc cgccgctacc
     6781 tccgctgcgc cagcgagtac atgatgggca tggcggacaa ggcgtccctg gagaaggcgg
     6841 acctcctgtg caaggagctg gggatcgggc ccgagtaccg gcccgttgta cccgccgccc
     6901 gccgggccgc cgaggaggcc gcgaccgtcg ctgagtcgac ggtcccggga gccgccgctc
     6961 cccacggggt ggattccgcc ttctgcgggg cggccctcca gcttccggga ggggaaatcg
     7021 tcacgggcaa gaactcccgc ctcctccacg cggccaccgc cgccgtcctg aacggggtca
     7081 agcgcctggc gggtattccg gactcgatcc ccctgctttc gcccctggtc atcgagtcca
     7141 tcgagaagct caagagcgag atcctccggg gacggaatcc cagcctggac gtggacgagg
     7201 ctctgatcgc cctgtccatc ggggccgctc tgacccccat ggtccagtcc tgcctggacc
     7261 agctccaccg cctgcggggc tgccagatgc acctttccca cgtgcccagc cccggagacg
     7321 aggcgggcct gcgcaagctg gggatcgagc tgacctccga cccggtctac gcctccaagc
     7381 ggctcttcgt ggagtgaggt cccctgtccc gaagcttgtg aataaattcg aagattccga
     7441 ttatgttact attgacagaa acgaactccc ctggtattct cccgtaacgt tcttataggg
     7501 agaaacacca tgagcgacta ttttaagacg tatccggacg ctaagggcaa ctacgggcag
     7561 tacggcggca gcttcatccc ccccaacctc caggcggaga tggagaagat cacggacgcc
     7621 tactactcca tcagcaagtc ccatgagttc atctccgagc tccggagcat ccgcaagcat
     7681 ttccagggcc ggcccacgcc ggtgtatttc gcaaagcggc tgtccgacga gtacggcggc
     7741 cggatctacc tgaagcggga ggacctcaac cacacggggg cccacaagct gaaccactgc
     7801 atgggcgagg ccctcctggc caagtacctg ggcaagaaga agctgatcgc cgagaccggg
     7861 gcgggccagc acggggtggc cctggccacc gcggccgcct acttcggact ggaatgcgag
     7921 atccacatgg gcgaggtgga catcgcaaag gagcacccga acgtcgtccg gatgaagatc
     7981 ctgggcgcca aggtcgtccc cgtctcccgg ggcctgagga ccctgaagga ggcggtggac
     8041 tccgccttcg aggcctacat ggccgacccg gtgaccgcca tctactgcat cggctccgtc
     8101 gtgggtcccc accccttccc catgatggtc cgggagttcc agcgggtggt gggcatcgag
     8161 gccaaggagc agttcttcga gatgacgggg gagcacccgg acagcgtgat ggcctgcgtg
     8221 gggggcggca gcaacgccat gggcatcttc tccgcctaca tcgaggacca gaacgtgaag
     8281 ctctacgggg tcgagcccat gggaaccggc accaagaccg gggagcacgc cgccacgatc
     8341 acctacggca agcccgggat gatccacggt ttcaagtgct acctcctcca ggacgagcag
     8401 ggccagccct cgccggtcta ctccatagcc agcggcctgg actaccccgg ggtgggaccc
     8461 gagcactcga tgctccacga cctgggacgg gtgacctaca cctccgtgac cgacgcggag
     8521 gcggtggacg ccttcttcca gctgagccgc aaggagggga tcatccccgc cctggagagc
     8581 gcccacgcgg tggcgtacgc cctcaagctg gcccgccggg aaccccggcg ctccatcctg
     8641 gtcaacctga gcggccgggg ggacaaggac atcgacttcg tggtggaaaa gcacggggag
     8701 cagtacggca tctgagcgcg gtgtcgggcc ggggcggttc gccccggccg agccggccgc
     8761 cccgggcccg ggagttccta taggttctcg atcagctccc aggcggagct cttccgggtt
     8821 ccctccacgt gcccggcgtg caggaactcc atcaccccgg tcttgaggac ctcgatggcc
     8881 ttgtcgatgt ccgtgatccg gaagatgaag accgccttgt tctcggcttt ctccccgaac
     8941 ccgtagacgt actcgatgtt gaggttgttg gccttgagga tctccaggat cttcataagt
     9001 cccccgggcc ggtcctcggc cttgagggcg aagacctcga cctccttgag gaagtacccc
     9061 cgttcccgga ggagcccgac ggccttgtcg ggcttgtcca cgatcaggtg gaccgagctg
     9121 aactccgtct cctcgctgac gaagatgctc cggatgtcga tgtccccctt gtgcaggacc
     9181 tgggacaggg catagagctg gccggccgtg ttttccagga cgacgctgat ctgcttgatc
     9241 ttcatgacag actccttgcg ggataaccgg cccggtccgg ttcagttcac catggagatg
     9301 ggctggcggg tggcctcgat ggcgtccctc cagaggctgc tctccggatc gaccttcctg
     9361 cgctgggcga tggccacgcc ggtggggatg tggacgaact cgttgttcac catcccgatg
     9421 agtatccggg tcttcccggc catggccgcg tgcacggcgt tgctgccaag ccggtcgcag
     9481 tagaccgagt ccacggggct ggccggggag gaccggatga tgtagctggg gtcgatgtac
     9541 ttgaggttga cctcgatccc cttcttcttg aaatgggcgt tgatcgcgtc cttgaggaag
     9601 atcccgatgt ccccgagctt gcggttcccc gaggcgtccg taccctccgc ggcggaatac
     9661 ttctgccccg ccccctcggc tgccacgatg accgcgtggt tccgctcggc cagacgcttc
     9721 tccaaatggg cgaagaggcc gttggggcct tccaggtcga aatccacctc gggtaccagg
     9781 acgaagttca cctcgtggac cgccaggacc gtgtgggcgg cgatgaagcc ggattctcgg
     9841 cccatcagct tcacgagccc tatgccgttg atggaggagt gggcctccgt atgggccgcc
     9901 gtcaccgcat gggaggcctg ggccacggcg gtgtcgaacc cgaagctccg gtccacgaac
     9961 agaaggtcgt tgtcgatggt cttggggatt cctaccaccg cgaccttgag gttccggcgc
    10021 tcgatctcct cggcgatggc cagggagccc ttctgggtcc cgtccccgcc gatggtgaac
    10081 aggatgttca cgttcaggcg ctccagggag tccacgatgt ccgaggtccg gtccccgccg
    10141 ccccgggagg agcccaggac ggtgcccccc agcttgtgga tgtcgtccac gacgtcggga
    10201 ttcaggggcc ggatgtcgta gccgtagtcc gggatgaggc cccggtaccc gaagcggacg
    10261 ccggagatgc gccgcacccc gtagcggtac cagaggcagc ggaccacgga ccggatcacg
    10321 tcgttcaggc ccgggcagag tcccccgcag gtgacgatcg cggcgtggac gtgctggggg
    10381 gcgaagtaga gtttttcccg ggggcccgcc ttctcgaaga actgggtcag gtcggtgacc
    10441 tccccggccc ggacgtggac gtcgtggcgc accaggtcct cgtcccggat gtagttggcg
    10501 atcccgtccc cgaccgtgtt ggagagcttg atgggggact gcagggagca cttgccgagg
    10561 ttgggcacca gtcgatcgct cacggggacc tccttccggg acagggatat tgtagaggaa
    10621 ggaggagggt ttttccacgg gggaaccgta atctcaggcc aggagacgtc cgccctcccg
    10681 gaccaccacc tcggacaggt cccggagggt gtcggactgg tacttccaca cgtgccagta
    10741 cagggggatc tccgtgcgca ggcccggatc caggtccacc agggtcccgt cggccaaccc
    10801 gggcaggacc ttgctcttcg ccacgaggcc gtagcccagg cccgcccgga tcgcctcgaa
    10861 gtacgcgtcc gcgaccggga catagtgggc cgggggcgtg acctggggat ccccgaaggc
    10921 cttgtagaga acccggagct ggaaccggtc gttccggtcc aggttcacaa ggggagcccg
    10981 cgtcgcggct tccctggaaa acccttcggg gaaccatcgc cgcgagaact caccggacgc
    11041 cacgaggacg taccggaggg acccgatccg ggtcgcggtg cagccctcca cggcgtcccg
    11101 ctccgaggtg acgcagcccg ccacggcccc ggatctcagg tatcgcagag tctgctcgtg
    11161 cccgtccacg aagagttcca acgtggtacc ggagcttcgg gcgaaggcgg cggcggcttc
    11221 caggaaccag acgaagaggc tgtccgcgtt gaccgccacg ggcacgtggg ggcggcaggc
    11281 cggagacttg gcgctgaccg cgtccacggt ctcgccctcc agggccagga cctggcggta
    11341 gtggcgcagg agccgggctc ccgcctcggt gggccggggc ggggtgtccc ggatgaccag
    11401 ggcccggccg atctgatcct ccagctgttt caccctctgg gataccgcgg attgggtgat
    11461 gcacaggcgg tccgcggccc gggcgaatcc tccctcgtcc agcacggcgg cgaaggcctg
    11521 gaccagctga tagtcgagca tggcgtatat taccacccct tctcgggtat cggaaaagat
    11581 aatttcacaa aaccccttcc gcggcgcata ctggaccggt caggaggcgc accatgaacg
    11641 accccaggct ttcggctatc gaggatatcc ggctcacctt cgacgaactt ccccccgacc
    11701 cggtctttgt ccccggcatc cgccgggccc cccgcaggga cgcccccctc acccctgccc
    11761 aggaaatcct ggccctcaag aacgcccttc ggtacgtccc ggagcgctgg cacgagaagc
    11821 tggctccgga gttcctcgag gagctccgga cccggggacg gatctacgga taccgattcc
    11881 gcccctctgg gcccctcaag ggaaagccca tcgactccta ttcgggccgc tgcgtcgagg
    11941 ggaaggcttt ccaggtcatg atcgacaaca acctggactt cgacgtggcc ctctatccct
    12001 acgaactcgt gacctacggg gagaccgggc aggtctgcca gaactggatg cagtaccgcc
    12061 tgatcaagaa gtacctggag gtcctggacg agaactccac cctggtggtc gagtcggggc
    12121 accccctggg gctcttccgg agccaccccc tggccccccg ggcgatgatc accaacggac
    12181 tcatgatcgg gatgttcgac aactccaagg acttcgccgt ggccgcagcc ctgggggtgg
    12241 ccaactacgg gcagatgacc gcggggggct ggatgtacat aggcccccag gggatcgtcc
    12301 acggtacctt caacaccctg ctcaacgcgg gacgcctcaa gctgggcatc cccgcggaca
    12361 aggacctggc cggccggctc ttcgtctcca gcggcctggg ggggatgtcc ggggcccaag
    12421 ggaaggccgc ggtcatagcc ggggccgctt gcgtgatcgc cgaagtggac cccagccggg
    12481 tggagacgcg gctttcccag ggctgggtgg acgcggtcga ggagaccccc gaagccgcct
    12541 tccgccgggc cgaggaggca cggaccgcgg gcaagccgat cgccgtggcc tacctcggca
    12601 acgtggtcga tctcttggaa tgggccatcg cgaagaacgt caagatcgac ctcctgtccg
    12661 accagacctc ctgccacgcg gcctacgagg gcggctactg ccccgcgggg ctctccttcc
    12721 cggagcgcac ccggatgctc aaggaggatc cggcccggtt ccgcaccctg gtggacggat
    12781 ccctccgccg tcacttcgaa gccatccgga ccttggccgc ccgggggacc tacttcttcg
    12841 attacggaaa ctccttcctc aaggccgtct gggacgcggg agtcaaggaa gtgtcccgca
    12901 acggcacgga cgagaaggac ggcttcatct tccccagcta cgtggaggac atcctggggc
    12961 ccgagctctt cgactacggg tacggcccct tccgctgggt ctgcctgtcc ggcaagcccg
    13021 agg
//
LOCUS       DAOTEI010000098        12430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3562_length_12430_cov_6.061818, whole genome shotgun sequence.
ACCESSION   DAOTEI010000098 DAOTEI010000000
VERSION     DAOTEI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12430
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3562_length_12430_cov_6.061818"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="P5313_10270"
     CDS             <1..709
                     /locus_tag="P5313_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY80790.1"
                     /translation="WDHIMGFPMFTPIYVPGTELRIRGPITYEEDPLEKVIGSQLSYR
                     YWPVRQEELAAKIIYEQIKEATFDLGDGIWLTTKYLNHPILCLGYRFEYRGRSFVTLY
                     DNEPFRNVFPTDPSDPQYEEAYAREGEEAAREQNDKILAFMSGADLLVHDTQYTAKEY
                     VSGKVGWGHSSFEHAINAAHKAGVKRLLLFHHDPNRTDTELQELEIFYQARLRGRTSM
                     NVEMSREGTLYTIDSVE"
     gene            733..1095
                     /locus_tag="P5313_10275"
     CDS             733..1095
                     /locus_tag="P5313_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY80791.1"
                     /translation="MKALIADDDSDNREILVRMLAHAGWEADAVGGGGEAVVRVLSGG
                     YDIALLDLAMPGVDGFEAARRIRAGGSRIALVAVSGAEAEERARESGFDRCLEKPYTL
                     EDLRGVLRDIAGTAGRET"
     gene            1198..2385
                     /gene="pgsB"
                     /locus_tag="P5313_10280"
     CDS             1198..2385
                     /gene="pgsB"
                     /locus_tag="P5313_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HRY80792.1"
                     /translation="MRLLLFAATSFLAFLAALAAERLRVARDRRAVPLRIGVTGTRGK
                     SSVVRLVAAALRGSGRRTLAKTTGSRPRLILPDGTERDFPRFGPPTILEQKRLLRTAR
                     AEGAEALVAEIMSVRPECQEVESAGILGIGVCVITNVRVDHEAEQGASRSEVARALAR
                     SVPREGRVVCSSGEDLPELRAAAARAGAELRVAPTRIPEELLPFLPALGYAEFEENLR
                     LALETCLALGLDGAAALRSMASAIPDLGALRVWRWEEEGRVRVFVNAFAANDVASTRA
                     VLDRSLAGKPAGARAAGLLNLREDRAERTRQWLRALEGGLPEPLEELFVLGPRSGAVR
                     RRLAARGVPCRALRDRDPEGILRSAAGGRPGELWIVGMGNIAGPGARIVEYCAERGSP
                     YGP"
     gene            2375..2809
                     /locus_tag="P5313_10285"
     CDS             2375..2809
                     /locus_tag="P5313_10285"
                     /inference="COORDINATES: protein motif:HMM:NF025467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HRY80793.1"
                     /translation="MDPETLLIGFLVAVVFHELTGIYPGGIVVPAYLALHAGEPARLA
                     GTAAAAAIGLGAYRFLSARLILFGRRGFFVLVTVSGLAALASGLVVPALLPEAAGWRA
                     VGIVVPGLLADACRKQGTLVTGSAAFAAMTVTYLLSRLLLVR"
     gene            2820..3905
                     /gene="pgsW"
                     /locus_tag="P5313_10290"
     CDS             2820..3905
                     /gene="pgsW"
                     /locus_tag="P5313_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HRY80794.1"
                     /translation="MKSARTILWILAALLSAAALSVERRITAYAPSPHYDEMVRAAEL
                     MRDAAERVRAARAAAGFDLDILRDPNRTGLVGVEYSETTTSLGDLEAKRTSASPDAAA
                     LAVLLLREAGVERGDAIAVGASGSFPGLSLAVLSAARALDLDVALVASLGASSWGANV
                     PGFSYLRIHEAAQPVLGYPIRAVSLGGGGDTGSDMQPEGRRLLSREISRSGLRFLDPR
                     SLEDGVAERLALYDAFLDGRTCAAFVNVGGASANIGEDAESLELAPGVNDAGNLSASG
                     TGAVFGMAARGVPVIHLLNLRALARDYGLPWDPSPFPEIGESRVYRVYDRNLYRRRLG
                     ILAVLYAAGLGVLGAAGRWLRRKFAGG"
     gene            3977..5617
                     /locus_tag="P5313_10295"
     CDS             3977..5617
                     /locus_tag="P5313_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY80795.1"
                     /translation="MKTRRTSARLSARLGLIAAACLASGACLARAAAGIPGVPGVPAS
                     PLLPAAAGAVLFAAAALLARGLARLPDRIRERTLAEIGEALGAEGTAESPGPDGAGSG
                     KDGLVSAEDLAVRIRADRERISAQRRASAMETERDLAAAHEREELKVRLDASVRSRLS
                     TVDATLEEVRSSADRLLEASERTRGTGGEVETAASAVSEILSEVTAALRDLRESAGRI
                     SREMYRTSEISETAVSAIAGATGSVEELEASTVRIGEIVGLIDEIAERTNLLALNAAI
                     EAARAGSAGRGFAVVAGEVKKLAAQTGKATLDIKALIDGVQAGARRARESSSATKEAV
                     GRLDEVISGVAAAVTSQEAAVGRVESGAAGLAAANEQVRSGARGLAAEVGETESLAEG
                     IHRIAGRIAEEGGGLRKEVDGFLEFIARKTSQDAKSLTIQAARYLAENGLEAAERTFL
                     SEGRFRFGDIYACVVDGEGRWVIYPPKPENKGQSILGFVDPDGVRIGERILGAGKLGE
                     GWTEYKWNNPLTGRVQDKLTYVKAVPDTDLFVYVGIYV"
     gene            complement(5760..7064)
                     /locus_tag="P5313_10300"
     CDS             complement(5760..7064)
                     /locus_tag="P5313_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRY80796.1"
                     /translation="MAEGKKGALGWYFNSNLLTRILIGLVLGAIVGIILGFTPGAVKG
                     YVSFVQPFGDLFVRLLKMIVVPVILFSLIAGAASIAPSRLGRVGVKIMVYYLFTSALA
                     VLIGLVFANIFQPGAGFNIVGAADIKGKEAVVQPLATILLNIIPTNPMESLAKGDVLP
                     IIFFAVVFGLGISFVKDSKTPAIAKGADMLFDVCNAAAETMYKVVKGIMQYAPIGVFV
                     LISIVFAQQGPKAIGPLLIVTLTVYLGLLVHLVGVYGGFLAVYKLGLIKFLKGANEAM
                     VTAFVTRSSSGTLPITLRVAEEKLGIPRATSSFTLPLGATINMDGTAIYLGVCAMFIG
                     YAIGRPLTFNQQLTVIITATLASIGTAGVPGAGAIMLLMVLESIGLKVEAGSAVAAAY
                     AMILGIDALLDMGRTCLNVTGDLAGTAIVSKTENELDLAKWR"
     gene            complement(7287..7946)
                     /locus_tag="P5313_10305"
     CDS             complement(7287..7946)
                     /locus_tag="P5313_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY80797.1"
                     /translation="MKTDSGTGRTAVAIVEDEREVREGLRYFLGLDERIRVAGSFGRA
                     EDFLAWMAENPEPDIVLMDIHLPGMDGIEATRAVLERHPDLVVLILTIFEEEDKILDS
                     IKAGAKGYILKNTKPEQLLEQILSAKAEGSPISPTVARRILEELRRGGTGKAADYGLT
                     PREREVLRDIVDGLTYRELAERHHIAGSTAKKHILHIYQKLNVSSKAEIVRKALDERL
                     I"
     gene            complement(7933..9735)
                     /locus_tag="P5313_10310"
     CDS             complement(7933..9735)
                     /locus_tag="P5313_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRY80798.1"
                     /translation="MTEGRWGISQKFILFISLSVLVFLVGIFLITRNVLGDYALATAD
                     ELSETILDQTDKRLGQFFEEMEHLARGLAGTRAVLDADEAAMGDLFVATVLPRARYLR
                     AVYLGTEDGRMFEWGTGKGFVDYRPSFPPGYDPRVRPWYRTAIEKGDFAISDPYIYAS
                     VDDIGITCALPVTGPDGSRVGVLGLDILLEDLRRILEGLEIPKQGKALLVSSTGVVVA
                     SQFPGDRAADPSQKGFALPFSLEEIRGPAGSFTAGIEGEETHFVYKRTSRADWTIAVG
                     MPLGSILESMRSLLNIISMVEIILMLMLTLALAGITGRLILSPLAGIVAVINRLKAGD
                     KAARVQVRSGDEFGVLGDEFNMLVDEVDGYSRNLEEKVRQRTEEIWQLQKENTQLRVA
                     EERRRIYRDMHDTIGAKLTNIFFCGGVAKDQAKEGPAKLREMLERIEENCLEAVASLR
                     GIVQGMHEDDRMGSDLVKFVSAGIRQRLESRDIDFDCRIRNRRALRDLGDEPRTEIEK
                     VLEELVSNTLRHSGASRVRLRLSSGSRGILLRFSDDGVGFDPAETRAGSFGLANVRFR
                     VERLGGKVSVRSVPGRGVEYSALLPARGEDHEDR"
     gene            complement(9845..10726)
                     /locus_tag="P5313_10315"
     CDS             complement(9845..10726)
                     /locus_tag="P5313_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY80799.1"
                     /translation="MSDGTGSPRGGVCVVTGATSGIGLAAACALARLGARVIGVGRDA
                     GRAGAALRRVEAAAAESGAPAPSFELADLSSLRETRALGERLASGSGGIDALVNCAGV
                     FTSRRTLTPEGLETQFAVNHLAPFLLTTTLLPRLRAAPDGRIVVVSSASHRFGRIRWE
                     DPSRARGYTGLGAYGQSKLGNVLFVRELARRLGSGSSVTVFAADPGLVRTDMGLKHGP
                     SLASLGWRLRRRAGTSPEVPAAAIALLVASPELRGRTGLYWKDGRELAPSPRALDARA
                     ARRLWDLSEGITREILG"
     gene            10858..>12430
                     /locus_tag="P5313_10320"
     CDS             10858..>12430
                     /locus_tag="P5313_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY80800.1"
                     /translation="MKTLSVSLRWKLIVPLAVSILIGLGFAAVIVLGVSEDSRRLGES
                     VRNLELLRSASILIQELEDERALAELYMSGKAMKVELDAQYQETDTKVSDFVTRMMAS
                     RIPDVVKEKAQRGIDSVADFRTRVDPGNKYVSFRNLYSISLGALTAIMPLIAQEERGT
                     EFGSPVSTIILLEEAKDAAHILRTVIPPILESGQPVTLGEATGLLDTLARMIVNLDSP
                     AISLTAGQSALGILRDLSARRLAEGAVRRIYMSYSQGNYDFKDDQFYAQLTAFVDMLA
                     VTIQAEIDQQLGVARAYAAEGRKTFLVVTVSVAAGYTVVALLALAVILSVVRSARSVS
                     ASLREIAQGGGDLTTRIAVKTRDELGQLAGYFNDFQEALARMVREIREAAAALSDVGA
                     ELSATMEETASAAVQISANVESVRRRTVDQSAGVTESSATVERIVENLRSLNRVIERQ
                     SEAVANSSASIEEMVANVRSVTGNIERLGGEYVRLTQSAGEGKSVLDRTVQDVKGIAD
                     RSERLGDANALIASIA"
BASE COUNT         1860 a         4265 c         4374 g         1931 t
ORIGIN      
        1 ctgggaccac atcatgggct tccccatgtt cacgcccatc tacgtgcccg gcacggagct
       61 ccggatccgg ggccccatca cctacgagga ggaccccctc gagaaggtta tcgggtccca
      121 gctctcctac cggtactggc cggtacgcca ggaggagctg gccgccaaga tcatctacga
      181 gcagatcaag gaagccacct tcgacctggg ggacgggatc tggttgacca ccaagtacct
      241 gaatcatccc atcctctgcc tgggctaccg cttcgagtac cggggccgca gcttcgtgac
      301 cctctacgac aacgagccct tccggaacgt cttccccacg gacccctcgg atccccagta
      361 cgaggaggcc tatgcccggg aaggggagga ggccgcccgg gagcagaacg acaagatcct
      421 ggccttcatg tccggggcgg acctcctggt ccacgacacc cagtacaccg ccaaggagta
      481 cgtctccggc aaggtcggct gggggcattc ctccttcgag cacgccatca acgccgccca
      541 caaggccggg gtcaagcggc tcctgctctt ccaccacgac ccgaaccgga cggacacgga
      601 gctccaggag ctggagattt tctaccaggc ccggctccgg ggccgcacgt cgatgaacgt
      661 ggagatgtcc cgggagggca ccctctacac gatcgactcc gtggagtagg cccggatgcg
      721 gaggacggga cgatgaaagc cctcatcgcg gacgacgact cggacaaccg cgagatcctg
      781 gtccgcatgc tggcccacgc cggctgggag gccgacgccg tgggcggcgg cggtgaggcc
      841 gtggtccggg tactgtcggg gggctacgac atcgccctcc tggacctggc catgccgggg
      901 gtcgacggct tcgaggcggc ccgccggatc cgggccgggg gatcccggat agccctggtg
      961 gccgtatcgg gggcggaagc cgaggagcgg gcccgggaga gcgggttcga ccgctgcctg
     1021 gagaagccct ataccctgga ggacctgagg ggcgtgctcc gggacatcgc cgggaccgca
     1081 ggccgggaga cctagccaga ccgaggctcc gtcccggccg agtctcgggg gctcctccgg
     1141 ggccaccgca gcccggacag cctgtacaag ggttccccga ccgggtactg tttgcccgtg
     1201 cgactcctgc tcttcgccgc aacgtccttc ctcgccttcc tggccgccct ggcggccgaa
     1261 cgcctgcgtg tcgcccggga ccgccgggcc gtgccgctcc ggatcggggt caccgggacc
     1321 cggggcaagt ccagcgtcgt ccgtctcgtc gccgcggccc tccggggttc cgggcggagg
     1381 accctggcca agaccacggg gtcccggccc cggctgatcc tgccggacgg gacggagcgg
     1441 gacttccccc gcttcgggcc gccgaccatc ctggagcaga agcggctcct ccggacggcc
     1501 cgggccgagg gggcggaggc cctggtcgcg gagatcatga gcgtgcggcc ggagtgccag
     1561 gaggtcgagt ccgccgggat cctggggatc ggggtttgcg tgattacgaa cgtccgggtc
     1621 gaccacgagg cggagcaggg ggcctctcgg tccgaggtgg cccgggccct ggcccgctcc
     1681 gtgccccggg aaggccgggt cgtctgctcc tccggggagg acctccccga gcttagggcc
     1741 gccgcggccc gtgcgggggc ggagctgcgg gtcgccccga cccggattcc ggaggagctc
     1801 ctgccgttcc tgccggccct gggctacgcg gagttcgagg aaaacctccg cctggcccta
     1861 gagacctgcc tcgccctggg gctggacggg gcggcggccc ttcggtccat ggcctccgcg
     1921 atcccggacc tgggggccct ccgggtctgg cgctgggagg aggagggccg cgtccgggtc
     1981 ttcgtgaacg ccttcgcggc caacgacgta gcctccaccc gggcggtcct ggaccggtcc
     2041 ctggcgggaa agccggcagg agcccgggcc gcgggcctgc tcaacctccg ggaggaccgt
     2101 gcggaacgca cccggcagtg gctccgggcc ctggaagggg gacttccgga gcccctggag
     2161 gagctcttcg tgctgggccc gcggtcgggg gccgtccggc ggcgcctagc ggcccggggc
     2221 gtcccctgcc gggccctccg ggaccgggat cccgagggga tcctccggtc ggcggcggga
     2281 gggcgtcccg gggaactgtg gatcgtcggt atggggaaca tagccgggcc gggtgcccgg
     2341 atcgtggaat actgcgccga gagaggcagc ccctatggac cctgagaccc tgctgatcgg
     2401 attccttgtg gccgtggtct tccacgagct gaccgggatc tacccgggcg gcatcgtggt
     2461 gcccgcctac ctggccctcc acgccgggga gccggcgcgc ctcgcgggca cggcggccgc
     2521 ggcggcgatc ggcctcggag cctaccggtt cctgtccgcc cggctcatcc tcttcggccg
     2581 ccggggcttc ttcgtgctgg tgacggtttc cggactggcc gccctggcct cgggcctggt
     2641 cgttccggct ctcctgcccg aagccgcggg atggcgggcc gtcgggatcg tggtccccgg
     2701 cctcctggcg gacgcctgcc ggaagcaggg gaccctcgtc acgggatccg cggccttcgc
     2761 cgcgatgacc gtgacctatc tccttagccg cctcctcctg gtccggtagg gccgcgccgt
     2821 tgaaatccgc ccggactatt ctgtggatcc ttgccgccct cctgtccgcc gccgcccttt
     2881 ccgtggagcg gcggatcacc gcctacgctc cctcccccca ttacgacgag atggtccggg
     2941 ccgcggaact catgcgggac gcggcggaga gggtgcgagc cgcccgggcc gccgcgggat
     3001 tcgacctcga catcctccgg gatcccaacc ggacgggcct ggtcggcgtc gagtattccg
     3061 agacgaccac ctccctgggg gacctggagg ccaagcggac ttccgcgagt cccgacgcgg
     3121 cggccctggc ggtcctcctg ctccgggaag cgggggtgga acggggggac gccatcgcgg
     3181 tcggggcctc cggctccttc ccgggcctgt ccctggcggt cctctccgcg gcccgggctt
     3241 tggacctgga cgtcgccctg gtcgcgtccc ttggggcctc ctcctgggga gccaacgtcc
     3301 ccggattctc ctatctccgc atccacgagg cggcccagcc cgtcctgggg tacccgatcc
     3361 gggccgtctc cctggggggc ggcggggaca ccggttcgga catgcagccc gagggccgac
     3421 gcctcctttc ccgggaaatc tcccggtcgg ggctccggtt cctggacccc cgttccctgg
     3481 aggacggcgt ggccgaacgc ctggccctgt acgacgcctt cctggacggc cggacctgcg
     3541 cggccttcgt gaacgtcggg ggagcctcgg cgaatatcgg ggaggacgcc gagtccctgg
     3601 aactcgcccc gggcgtgaac gacgctggaa acctttcggc ctcgggtacg ggggcggtct
     3661 tcgggatggc cgcccggggc gtgccggtga tccacctgct gaatctccgg gcccttgccc
     3721 gggactacgg cctgccctgg gatccctccc ccttcccgga gatcggggaa tcccgggtct
     3781 accgggtgta cgaccgaaac ctgtaccgga ggcgtctggg gatactggcc gttttgtacg
     3841 ccgcggggtt gggggtactg ggggcggcgg ggcggtggct ccgaaggaaa ttcgccgggg
     3901 gatgaaatga ccacggtcat cgaccgcggt cgattccccg ttgcgggttt tcctgtatcg
     3961 gtttatcatc ctctccgtga aaactcgacg aacatcggcg cgcctctccg cgcgcctggg
     4021 gctgatcgcg gccgcctgcc tggcctcggg cgcctgcctg gcccgggcgg ccgcggggat
     4081 cccgggcgtt ccgggagtcc cggcctcgcc cctgcttcct gccgccgccg gggctgtcct
     4141 gttcgcggcc gcggcgcttc tggcccgcgg cctggcccgc ctgccggacc ggatccggga
     4201 acgtaccctg gccgagatcg gggaggccct gggggccgag ggaacggccg aatcccccgg
     4261 ccccgacggc gcgggctcgg ggaaggacgg actcgtctcc gcggaggacc tggcggtccg
     4321 gatccgggcc gaccgggagc ggatatccgc ccagcgccgg gcttcggcca tggagaccga
     4381 gcgggatctc gcggcggccc acgaacggga ggaactcaag gtccgcctgg acgcctccgt
     4441 gcggtcccgc ctttcgaccg tggacgccac cctggaggag gtgcggtcct ccgcggacag
     4501 gttgctggag gcttcggagc ggacccgcgg gacgggcggg gaggtcgaga ccgccgcgtc
     4561 ggcggtttcc gagatccttt cggaggtgac ggccgccctg cgggacctgc gggaatccgc
     4621 gggccgcatc agccgggaga tgtaccgcac ttccgagatc tccgagaccg cggtttccgc
     4681 gatcgcgggc gccaccggct ccgtggagga gctcgaggcg tccacggtcc ggatcgggga
     4741 gatcgtgggc ctgatcgacg agatcgccga gcgcacgaac ctcctggccc tcaacgcggc
     4801 catcgaggcg gcccgggcgg gttccgcggg caggggcttc gccgtggtgg ccggggaggt
     4861 gaagaagctc gcggcccaga cggggaaggc caccttggac atcaaggccc tgatcgacgg
     4921 tgtccaggcg ggggcccgac gggcccggga atcgagctcc gcgaccaagg aggccgtggg
     4981 gcgcctggac gaggtgatct ccggggtcgc cgccgcggtg accagccagg aggccgcggt
     5041 cggacgggtc gagtccggcg ccgccggcct ggcggcagcc aacgagcagg tccgatccgg
     5101 cgcccggggc ctggccgcgg aggtgggtga gacggagtct ttagccgaag gtatccaccg
     5161 gatcgcgggc cggatcgccg aggagggggg tgggctgcgg aaggaagtcg acggcttcct
     5221 cgagttcatc gcccggaaga ccagccagga cgccaagtcc ctgaccatcc aggccgcccg
     5281 gtacctggcg gagaacggcc tggaggccgc ggagaggacc ttcctgtcgg aaggccgctt
     5341 ccgcttcggg gacatctacg cctgcgtcgt ggacggagag ggccgctggg tcatctatcc
     5401 ccccaagccg gagaacaagg gccagagcat cctgggcttc gtggatcccg acggggtacg
     5461 gatcggcgag cgcatcctgg gggccgggaa gctgggggag ggctggaccg agtacaagtg
     5521 gaacaatccc ctcaccggcc gggtccagga caagctcacc tacgtgaagg ccgtcccgga
     5581 cacggacctc ttcgtctacg tggggatcta cgtgtagcgg ggggcgttgg gagcggaccg
     5641 gtgtccgtgt accgcgtagc gggaccgcag ggaaaaaggg ccgcccctcg gggcggccct
     5701 tttttagcaa tcgaggaagc ccgaagggct tccttgccgc taagtcggct atcgccgact
     5761 tagcgccatt tggccaggtc cagctcgttc tcggtcttgg acacgatggc ggttccggcc
     5821 aggtcgccgg tgacgttcag gcaggtgcgg cccatgtcca ggagggcgtc gatgcccagg
     5881 atcattgcgt aggcggcggc cacggcgctg cccgcctcga ccttgaggcc gatggactcg
     5941 aggaccatga gcagcatgat cgctccggct ccggggacgc cggcggtgcc gatggaggcc
     6001 agggtggcgg tgatgatcac cgtcagctgc tgattgaagg tcaggggccg gccgatggcg
     6061 tagccgatga acatggcgca gacgcccagg tagatggccg tgccgtccat gttgatcgtg
     6121 gcgcccaggg gcagggtgaa ggaggaggtg gcccggggga tgcccagctt ctcctcggcg
     6181 acccgcaacg tgatgggcag ggtgccgctg gaagaccggg tgacgaaggc cgtcaccatg
     6241 gcctcgttgg cgcccttcag gaacttgatc agcccgagct tgtagaccgc caggaatcct
     6301 ccgtacacgc cgaccaggtg caccaggaga cccaggtata ccgtcagggt cacgatgagg
     6361 aggggaccga tggctttggg tccctgctgg gcgaagacga tggagatgag gacgaacacg
     6421 ccgatggggg cgtactgcat gatccccttc acgaccttgt acatggtctc cgcggcggcg
     6481 ttgcagacgt cgaagagcat gtccgcgccc ttggcgatgg cgggggtctt ggagtccttc
     6541 acgaaggaga tccccaggcc gaagaccacg gcgaagaaga tgatcgggag gacgtcgccc
     6601 ttggccaggg actccatggg attggtcggg atgatgttga gcaggatcgt cgcaaggggc
     6661 tggactacgg cctccttgcc cttgatgtcc gcggctccca cgatgttgaa gcccgcgccg
     6721 ggctggaaga tgttcgcgaa caccaggccg atcaggacgg ccagggcgga ggtgaacagg
     6781 tagtacacca tgatcttgac gcccacccgg cccaggcggg agggggcgat gctggccgca
     6841 ccggcgatca gggagaagag gatgaccggt acgacgatca tcttgagcag ccggacgaag
     6901 aggtccccga agggctggac gaagcttacg tagcccttca ccgccccggg cgtgaagccc
     6961 aggatgatcc cgacgatcgc gcccaatacg agaccgatca ggatgcgcgt gagcaggttg
     7021 gagttgaagt accagcccag cgcgcctttc ttgccttccg ccatacatgc ctccgggaaa
     7081 tcgaggatgg tccggccgat ccccgggggg cggcgcggac ggaatcaccc cagtgtaagg
     7141 ctgaattccg aagcccgata tgcaccaaag ggagtatatt tacgtgcggg ccgcgttcgg
     7201 ttcggctccg cgtacaccct tcggtccaag cggcaccccg cggcccggca ttcccccggg
     7261 tccgggcccg ggcccgaatt cccgatctag atcagccgct cgtccagggc cttgcggacg
     7321 atctccgcct tggaggagac gttcagcttc tggtagatgt ggaggatgtg cttcttggcg
     7381 gtggatccgg cgatgtggtg ccgctccgcc agctcccggt aggtgaggcc gtccacgatg
     7441 tcccgcagga cttcccgctc ccgtggggtg aggccgtagt ccgcggcctt tcccgtgccg
     7501 cccctgcgga gctcctccag gatgcggcgc gccacggtgg ggctgatggg cgagccctcg
     7561 gccttggcgc tcaggatctg ctccaggagc tgctcgggct tggtgttctt gaggatgtag
     7621 cccttggcgc cggccttgat ggagtccagg atcttgtcct cctcctcgaa gatggtgagg
     7681 atgaggacca cgagatccgg gtgcctttcc agcaccgccc gggtggcctc gatcccgtcc
     7741 atcccgggaa ggtggatgtc catgaggacg atgtcgggct cggggttttc cgccatccag
     7801 gccaggaaat cctccgcccg gccgaaactg cccgccaccc ggatacgctc gtccaggccc
     7861 aggaagtacc ggaggccctc ccggacctcc cgctcgtcct ccacgatggc cacggcggtg
     7921 cggccggttc cgctatcggt cttcatggtc ctcccctcgg gcgggaagca gggccgagta
     7981 ttcgaccccc cggcctggga ccgagcgcac ggaaaccttc ccccccagcc gctccacccg
     8041 gaaccggacg ttcgccaggc cgaaggaccc cgcccgggtc tccgcggggt cgaagcccac
     8101 gccgtcgtcg gaaaaccgca gcaggatgcc ccgggatcct gacgagagcc ggagccggac
     8161 ccgggacgcc ccggagtgcc tcagggtgtt gctgaccagc tcctccagga ccttctcgat
     8221 ctccgtgcgc ggctcgtccc cgagatcccg gagggccctg cggttccgga tccggcagtc
     8281 gaagtcgatg tcccgggact ccagccgctg gcggatcccc gcggacacga acttcaccag
     8341 gtccgagccc atgcggtcgt cctcgtgcat gccctgcacg atgcccctca ggctggccac
     8401 ggcttccagg cagttctcct cgatccgctc cagcatctcc cggagcttcg cgggcccttc
     8461 cttggcctgg tccttggcca ccccgccgca gaagaagatg ttcgtcagct tggccccgat
     8521 ggtgtcgtgc atgtcccggt agatccggcg gcgctcctcc gccacccgga gctgggtgtt
     8581 ttccttctgg agttgccaga tctcctcggt ccgctggcgg accttctcct ccaggttccg
     8641 ggaatacccg tccacctcgt ccaccagcat gttgaactcg tcgcccagga cgccgaactc
     8701 gtccccggac cggacctgga ctcgggccgc cttgtccccg gccttcagtc ggttgatcac
     8761 cgccacgatc ccggccaggg gcgagaggat gagccgcccc gtgatgcccg ccagggccag
     8821 ggtgagcatg agcatcagga tgatctccac catgctgatg atgttgagga ggctgcgcat
     8881 ggattccagg atggacccca ggggcatgcc cacggcgatg gtccagtccg cccgggaggt
     8941 cctcttgtag acgaagtgcg tctcctcccc ctcgatcccg gcggtgaagg aacccgcggg
     9001 tccccgtatc tcctccaggg agaagggcag ggcgaagccc ttctggctgg ggtcggcggc
     9061 ccggtccccg gggaactggc tggccacgac gacccccgtg gagctcacca ggagggcctt
     9121 cccctgcttg gggatctcca ggccttccag gatcctgcgc aggtcctcca gcaggatgtc
     9181 caggcccagg accccgaccc tcgatccgtc cggacccgtc accggcaggg cgcaggtgat
     9241 tccgatgtcg tcgacgctgg cgtagatgta ggggtccgaa atggcgaaat cgcccttttc
     9301 gatcgcggtc cggtaccagg gccggacccg gggatcgtag cccggcggga agctaggccg
     9361 gtaatcgacg aagcctttgc ccgttcccca ttcgaacatc cgtccgtcct cggtgcccag
     9421 gtagaccgcc cggaggtagc gcgcccgggg caggaccgtg gccacgaaga ggtcccccat
     9481 ggccgcctcg tccgcgtcca gcaccgcccg ggttcccgcc agcccccggg ccagatgctc
     9541 catctcctcg aagaactggc ccaggcgctt gtccgtctgg tccaggatgg tctcggacag
     9601 ctcgtccgcg gtggccaggg cgtagtcccc caggacgttc cgggtgatca ggaagatccc
     9661 caccaggaag accagcaccg agagggagat gaagagtatg aatttctggc tgatgcccca
     9721 tcgcccttcg gtcacccgtc gccctccatc gaatggcgga attatatagg cgcgccgacg
     9781 gcgggggcaa ggaaatccgg cgccggctcc tccggcgtcc cggaagccgg ggccggcgtc
     9841 cggcctagcc gaggatttcc cgggtgatcc cctcgctcaa gtcccagagc ctgcgggcgg
     9901 cccgggcgtc cagggcccgc ggggaggggg cgagctcccg gccgtccttc cagtagagcc
     9961 ccgtgcgtcc ccgaagttcc ggcgaggcaa cgaggagggc gatggcggcg gcgggcacct
    10021 ccggggatgt gccggcccgg cgccgaagcc tccaccccag actggccagg gaggggccgt
    10081 gcttgagccc catgtccgta cggaccagcc ccgggtccgc cgcgaagacc gtgacggagg
    10141 atccggagcc caggcggcgc gccagttccc ggacgaagag gacgttcccc agcttggact
    10201 gcccgtaggc gcccaggccg gtatagcccc gggcccggga cggatcctcc cagcggatcc
    10261 gcccgaagcg gtgggaggcg gagctcacga cgacgatccg gccgtccggg gcggcccgga
    10321 ggcggggaag gagggtcgtg gtcaggagga agggggccag gtggttcacg gcgaactggg
    10381 tctccaggcc ctccggggtc agggtccgcc gggaggtgaa gacccccgcg cagttcacca
    10441 gggcgtcgat ccccccggac ccggaggcca gccgttcccc cagcgcccgg gtttcccgca
    10501 ggctggacag gtccgccagc tcgaaggagg gagcgggcgc cccggactcg gcggccgcgg
    10561 cctcgacccg ccggagggcg gccccggccc gaccggcgtc ccgccccacc ccgatcaccc
    10621 gggctcccag ccgcgccagg gcgcaggccg cggccagccc gatgccggat gtggctcccg
    10681 tgacaacgca gaccccgccc ctcgggcttc cggttccatc cgacatccgc gaccctccgt
    10741 ttctacccgg ggaattgccc cgcgggtcga tcataccata ccgccggata tacggtattc
    10801 accatattag agattcaatg ctttctcgaa tccggatctg ggggtatact gtccccaatg
    10861 aagactctat ccgtttccct ccgatggaag ctcatcgtcc ccctggccgt ctcgatcctg
    10921 atcggcctgg gtttcgccgc ggtcatcgtc ctgggcgtct ccgaggattc tcgcagactc
    10981 ggggagagcg tccggaatct ggagcttctg cgttccgcct ccatcctgat ccaggaactc
    11041 gaggacgagc gcgccctggc ggagctctac atgagcggaa aggcgatgaa ggtcgagctg
    11101 gacgcccagt accaggagac ggataccaag gtctccgatt tcgtcacccg catgatggcg
    11161 tcccgtattc ccgacgtggt caaggagaag gcccagcggg ggatcgactc ggtggcggac
    11221 ttccggaccc gggtggaccc gggcaacaag tacgtgtcct tccggaacct ctactccatc
    11281 agcctcggcg ccctgacggc gatcatgccc ctcatcgccc aggaggagcg gggcacggag
    11341 ttcggctccc ccgtctccac catcatcctc ctggaggaag ccaaggacgc ggcccatatc
    11401 ctccgtaccg tgatcccccc catcctggag agcggccaac ccgtgaccct gggagaggcg
    11461 acgggattgc tggataccct ggcccgcatg atcgtgaacc tggattcccc cgcgatatcc
    11521 ctcaccgccg ggcagtccgc actgggcatc cttcgggacc tgtccgcccg aaggctcgcg
    11581 gagggcgcgg tgcgcaggat ctacatgtcc tacagccagg gaaactacga tttcaaggac
    11641 gaccagttct acgcccagtt gaccgccttt gtggacatgc tggccgtcac gatccaagcg
    11701 gagatcgacc agcagttggg cgtcgcacgg gcctacgccg cggagggccg gaagaccttc
    11761 ctggtcgtga ccgtgtccgt agccgcagga tacaccgtgg tcgccctttt agccctggct
    11821 gtgatcctgt ccgtggtccg gtcggcacgg tccgtgtcgg cctccctccg ggagatcgcc
    11881 cagggaggcg gggacctgac cacccggatc gccgtgaaga cccgggacga actgggacag
    11941 ctggcggggt acttcaacga cttccaggaa gccctggccc ggatggtgcg ggagattcgg
    12001 gaagccgccg cggccctatc ggacgtgggc gccgagctgt ccgcgaccat ggaggagacc
    12061 gcctccgcgg cggtacagat cagcgccaac gtggaaagcg tccgacgccg caccgtggac
    12121 caaagcgccg gggtgaccga gtcctccgcc acggtggagc gcatcgtgga gaacctccgc
    12181 tccctgaacc gtgtgatcga gcgccagtcc gaggccgtgg ccaattcctc ggcgtccatc
    12241 gaggagatgg tggccaacgt ccgctccgtc acggggaaca tcgagcgcct gggcggggag
    12301 tacgtccggc tgacccagtc cgcgggggag ggcaagtccg tcctggaccg gacggtccag
    12361 gacgtgaagg gcatcgccga ccggtccgag cgcctggggg acgcgaacgc cctcatcgct
    12421 tccatcgcgg
//
LOCUS       DAOTEI010000099        12420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3571_length_12420_cov_4.717994, whole genome shotgun sequence.
ACCESSION   DAOTEI010000099 DAOTEI010000000
VERSION     DAOTEI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3571_length_12420_cov_4.717994"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="P5313_10325"
     CDS             complement(<1..604)
                     /locus_tag="P5313_10325"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRY80801.1"
                     /translation="MTVREALSHGASVLSGTGSDTSYLDACLLLARAAGCGRAALLAR
                     LSDPLLPEAETTYSTFLSRRAEGESVAYILGEKEFFGRTFRVDHRVLVPRPETEILVE
                     TALRAAGRVPGGSASGADARGPGARRSGSGAGLRIHDAFTGSGCVGITLAAELPGSEV
                     SLSDLRPGARDVAARNAREILGRDLQILESDVLAGVSGPFD"
     gene            complement(601..1671)
                     /gene="prfA"
                     /locus_tag="P5313_10330"
     CDS             complement(601..1671)
                     /gene="prfA"
                     /locus_tag="P5313_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRY80802.1"
                     /translation="MIDKLEALAERHRELEVRVSDPDLVKDPAAYRDTMREHARLSET
                     MVLYDEYKHALSDLEGARSLGRDEKDPDMRAMAQDETRTLEARIGDLETRLRDRLIPR
                     DPLDDKNTIMEIRAGTGGDEAALFVADLFRMYSRYADSRKWKIEVMNLNETELGGYKE
                     IVFSISGESVYESMRWESGVHRVQRVPETEASGRIHTSAVTVAVLPEAEETEVDIRTE
                     DLRIDVMRAGGPGGQCVNTTDSAVRITHLPTGLVVHCQDEKSQIKNKAKALRILRARL
                     FDLEESRRQAERAAERKSQVGSGDRSERIRTYNFPQNRVTDHRVDLTLYKLDLVLQGD
                     MGEIVEALKASAREAVLKVDRP"
     gene            complement(1668..1847)
                     /locus_tag="P5313_10335"
     CDS             complement(1668..1847)
                     /locus_tag="P5313_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80803.1"
                     /translation="MKLEINPRGNGACPICTFTDDCRIKRLLIANADSLDGKSDESLE
                     IVIYGCPYFKEKADL"
     gene            1991..3265
                     /gene="serS"
                     /locus_tag="P5313_10340"
     CDS             1991..3265
                     /gene="serS"
                     /locus_tag="P5313_10340"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRY80804.1"
                     /translation="MLDYRFIKDNLAAVKKNVADRHMQADPDLVVELFDRRGELLRRL
                     EDERRARNDNAAAMKGKLPEEERRRLIEEGKALKDRIASLEAEYAEAEAHLDAEGRRI
                     PNMAHPSAPVGKEDKDNLEVKRVGKPTEFSFEPKDHVQLGQDLDILDFDTATRVSGTK
                     FYYLKNQGVILELALVRFALDRLQKKGFTPFITPDVAKTEILEGIGFNPRGAESNVYT
                     VEGEGACLIGTAEITLGGYHSGQVLDKAALPLRLAGVSHCFRREAGAAGQFSKGLYRV
                     HQFTKVEMFAYVLPEDSDRVHEELRGIEEEIFSALEIPFRVVDTCTGDLGAPAYRKWD
                     LEAWMPGRNGGEWGEVTSTSNCTDYQSRRLNVRYKDDDGKNKYVHMLNGTAIACSRAI
                     IAILENFQRADGSVRIPAALVPYTGFEEIRRA"
     gene            complement(3284..4153)
                     /locus_tag="P5313_10345"
     CDS             complement(3284..4153)
                     /locus_tag="P5313_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRY80805.1"
                     /translation="MVPILLALASILVWSTLALFTSQTASLPPLFSVGAALGIGGLAG
                     LFRVRDWRIPPATLAVGVGGIFGYHALLFAAFRLAPAVEVNLINYLWPLLIVVLSPVY
                     LRGFELRPGHAVGALLGLAGAFLVVGGGRFSLNPEGLPGHLMAAAAAFIWATYSLLTK
                     RVPPFSTGAVGLFCLISGALSLALWAGLDLAATGSVRLPPMTGREALFLIFLGLGPMG
                     LAFYTWDAALKRGDPRIIGSLAYLTPLLSTFNLVVAGGKTLTWATVAALALIMAGAVV
                     GSYGGRRRTGGTA"
     gene            complement(4177..4608)
                     /locus_tag="P5313_10350"
     CDS             complement(4177..4608)
                     /locus_tag="P5313_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80806.1"
                     /translation="MADERRGSGRRFGRRRKGRQDRPDRPAASREAEYAAPAAESQKP
                     NPPLDCAYCQKPIYDLSTALADPESGGPVHFDCAFQRMADRESLAPGEKLVYIGSGAF
                     AVVEFKDRGENAFTVKRRLAFEEEGKKQDWRRALSSRVSSL"
     gene            complement(4586..4756)
                     /locus_tag="P5313_10355"
     CDS             complement(4586..4756)
                     /locus_tag="P5313_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80807.1"
                     /translation="MSCPGERKHDPRRLEELRRKMQDEEYIAGAIQRIAQVLSAEIMN
                     GYGVQHGGRKAR"
     gene            complement(4753..5109)
                     /locus_tag="P5313_10360"
     CDS             complement(4753..5109)
                     /locus_tag="P5313_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRY80808.1"
                     /translation="MRRVEKGRLGEAAAAEYLLSEGWTIVDRNWRCPAGELDIVALRG
                     EDLAFVEVKTVDAYGPESADRLLGPAKRRHIVESSKYFLALKREYNRMRPRYDVILVR
                     GSAVCRHIPQAFLEHP"
     gene            complement(5128..6273)
                     /locus_tag="P5313_10365"
     CDS             complement(5128..6273)
                     /locus_tag="P5313_10365"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRY80809.1"
                     /translation="MKKKITLDQIKPGASFTEDVFIDDQNLLVPAGVAVKQKDLDMLQ
                     KWGVAFVFTEGSQIAGTASSSPDGPRADGVNPAAFLGDRELYRSYTDMVDMLKAVYDR
                     LGRMERVEPKTVDQISQDVLRVVRDKQGRAVAAILSGDMQGYSYARAGVNTAILSVVI
                     GVTLKLPPYRLSYLATGALLHDAGMLRIPEAIVRKKGHLSEEELQKMRAHPLLTYKII
                     TKELLYPDDVGIIGLQHHERWDGQGYPRRVAGQDIDVLSRIVSVADAFEAMVSEKPYR
                     NSMIGYAAMKALLSDNSRRFDPEILKAFIRSMGIYPIGSMVLLNNAAIARVVETHPDA
                     PLRPKLRILVDEFGKSFQDDKGDQVDLLSEKALFIARALDPKEVASG"
     gene            complement(6270..6563)
                     /locus_tag="P5313_10370"
     CDS             complement(6270..6563)
                     /locus_tag="P5313_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRY80810.1"
                     /translation="MLDEESPRIRPILAGRTKKAVALRYDDSLPAPFLVAKGAGASAE
                     RIRDLAEELGIPVLADEALAGFLFPLDPGDMVPEAYWEIVARVYVFIAKLEGI"
     gene            complement(6556..7740)
                     /locus_tag="P5313_10375"
     CDS             complement(6556..7740)
                     /locus_tag="P5313_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80811.1"
                     /translation="MERIGSDSTRSLQIRGAAGAPGPEVPGLREGASIVFKALAPSSE
                     GRWTVLVGGVRLDAAVAASLKPGSLYSARAERLPDGAWALRVLGPAVEGRASEFLRSS
                     GLPGDAASILALRALMAEGLPLEPRQIARLRTVLLKKEADEERAALLARALAKGLEPG
                     AYADAVDAASFADGRGEGGGRDPGGRREAQEGRDGGSREEAGGSGGERQDAPRGERPD
                     TGPGEDVPAGAPASGPVWKEYPGTGGDDPESPEALAGLLKTLAGRTGDRPDPRQVFNA
                     RSGPGGRWIYVPYRFERGGVAFSGTLRILVPRGSRSGAVLSADVHVDGPGRTGSYAFD
                     LRGEGNALRLGFADRSGSGLEGPAGGLGGLERSLADFGCRVEVLETGNRDAEYPAVDD
                     HA"
     gene            complement(7747..8142)
                     /gene="rplS"
                     /locus_tag="P5313_10380"
     CDS             complement(7747..8142)
                     /gene="rplS"
                     /locus_tag="P5313_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRY80812.1"
                     /translation="MNLIKKIEAAELREVPDFKPGDTVRVHFKIVEGKNERVQIYEGL
                     VMAIKNAGIGRSFMVRKNSYGVGVERVFPLNSPRIEKLEIVRYGKPRRAKLYYIREKV
                     GKKAKVKELLGGRKAPKKESAAAEAPAQA"
     gene            complement(8129..8869)
                     /gene="trmD"
                     /locus_tag="P5313_10385"
     CDS             complement(8129..8869)
                     /gene="trmD"
                     /locus_tag="P5313_10385"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRY80813.1"
                     /translation="MKLTVLTLFPEILKGFFESSIMKRAVERGLVTYDLVNIRDYATD
                     KHHKCDDEVYGGGAGMLMLPGPLGRALDAAGSPGVRTVFVTPGGRLFNQAYARDLARE
                     PKLVILCGRYEGVDQRIVDKYVSDEISIGDYVLSSGEVAALAVVDAVYRLIDGVITGE
                     SLAEESFEGGLLEYPQYTRPALYDSIPVPDVLVSGHHANITRWRLKKRLEKTLAYRPE
                     LLSDAMLSDDLRGLIERIAAEGGIDEPD"
     gene            complement(8866..9393)
                     /gene="rimM"
                     /locus_tag="P5313_10390"
     CDS             complement(8866..9393)
                     /gene="rimM"
                     /locus_tag="P5313_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRY80814.1"
                     /translation="MQNPSAGQEQDLLAIGRLGSPHGVQGFLRVQSYSGEVAHFADLG
                     EILLTREGERRTVRVEESRVSGPDVLLRLAGSACPEEARAWTGWEILVPRSRAAPLGP
                     GEYYIADLAGCDLVFAGASVGTVEAVLEGGEAPLLEISRTPARTVLVPFRKEFIGEVD
                     TAGRRIELLADWILE"
     gene            complement(9447..9680)
                     /locus_tag="P5313_10395"
     CDS             complement(9447..9680)
                     /locus_tag="P5313_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRY80815.1"
                     /translation="MERDLVEYIAKSLVDDPSAVSVTAVDGDKSVLLELRVAEGDVGK
                     VIGKHGRIAKALRTILSASASHSAKRVVLEILD"
     gene            complement(9724..9975)
                     /gene="rpsP"
                     /locus_tag="P5313_10400"
     CDS             complement(9724..9975)
                     /gene="rpsP"
                     /locus_tag="P5313_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRY80816.1"
                     /translation="MSVKIRLKKFGAKKRPYYRIVVMDSRLAREGSTLEEIGIYHPIE
                     SEDRQVVIDEAKARAWLAKGALPSDTVRSLFNKKNIRLG"
     gene            10394..11425
                     /locus_tag="P5313_10405"
     CDS             10394..11425
                     /locus_tag="P5313_10405"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRY80817.1"
                     /translation="MPARPSPLPPDALLLAPMVGLSHRPLRELVLGFGGCDLAYTEMT
                     SAAGYLSGAPYDRYFLDTGPEPGRAVLQFYAVDAPRMVQAARKAAREIPAAGLDLNFG
                     CSAPHIEKAGGGVSWMKDPGAARDLVARVRDARPEGGISAKLRVGYDEDYGRLRDFCG
                     GLAEAGADWLVLHPRLRSEKLRRTGRWDYVRRLGEDLDIPVVGNGDIRGPEDHAARVR
                     ECRPAGIMLGREAVRRPWVFALIRGLERDPGFQITVDIAGTAETALRLMEEHQPPEFH
                     ISRARRFFYYYCDNLSFAHHIRWKIQNAPDLKAIRQALAEYFEEVPGDRSQVTGARPQ
                     EPGDRGTSR"
     gene            11546..>12420
                     /locus_tag="P5313_10410"
     CDS             11546..>12420
                     /locus_tag="P5313_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY80818.1"
                     /translation="MAETLAQLFLEKVRDYPELNAQYSKNGGNEFKPTTYRQLLEEVA
                     SFAEALTESGVKRGDRVGLISDNRKEWLITDLALLGLGAADVPRGCDATDQEVRHILS
                     WSECRLTVVENDKQLKRVIDLRGQIPLLEKVIVYESVPEELLAAAKKAKLEVRTYAQA
                     MEEGKKLRAERPGRYEKETAAGKRDETATLIYTSGTTGEPKGVMLTHGNFLYQTDYIL
                     TIIDIKPGHIFLSVLPIWHSFERIAQYMILAAGAGVAYSKPIGAIMLADLQAIKPQWL
                     ASVPRIWESVKDGIYR"
BASE COUNT         2009 a         4293 c         4115 g         2003 t
ORIGIN      
        1 ggtcgaaggg cccagagacc ccggccagga cgtcgctctc gaggatttgc aggtcccggc
       61 ccaggatctc ccgtgcgttc cgggccgcga cgtcccgggc cccgggacgg aggtccgaca
      121 gggaaacctc cgagcccggg agctccgcgg ccagggtgat ccccacgcag ccggagcccg
      181 tgaaggcgtc gtggatccgg aggccggcgc cggatccgga ccggcgggcg ccgggacctc
      241 gggcgtccgc tcccgaggcg cttccgcccg gaacgcggcc ggctgcccgg agcgccgtct
      301 ccaccaggat ctccgtctcc ggacggggca ccaggacccg gtgatccacc cggaaggtcc
      361 gcccgaagaa ctccttctcc cccaggatat aggccacgct ctccccctcg gcgcggcggg
      421 agaggaacgt ggagtaggtt gtttccgcct ccgggagcag gggatcggac aggcgggcca
      481 gcagggcggc acggccgcag cccgcggccc gggccaggag gaggcacgcg tccaggtagg
      541 aggtgtcgga gccggttccg gaaagaacgg aggccccgtg ggaaagggcc tcccggaccg
      601 tcatggccgg tcgaccttca gcacggcttc ccgggcgctg gccttcaagg cctccacgat
      661 ctcgcccatg tcgccctgga ggaccaggtc cagcttgtac agggtcaggt cgacccggtg
      721 gtccgtgacg cggttctggg gaaagttgta ggtgcggatg cgctcggacc ggtcgcccga
      781 gcccacctgg ctcttgcgct ccgcggccct ctcggcctgg cgccgggatt cctccaggtc
      841 gaagagacgg gcccggagga tgcgcagggc cttggccttg ttcttgatct ggctcttctc
      901 gtcctggcag tggaccacga gccccgtggg caggtgggtg atgcgcaccg cggagtccgt
      961 ggtattgacg cactggccgc cgggtccgcc cgcccgcatg acgtcgatgc ggaggtcctc
     1021 ggtccggatg tccacctccg tctcctccgc ctccgggagg accgccacgg tcacggcgct
     1081 ggtgtggatg cggccggagg cttccgtctc gggcacccgc tggacccggt ggaccccgct
     1141 ctcccagcgc atgctctcgt acacggactc cccggagatc gagaagacga tctccttgta
     1201 gccccccagc tccgtctcgt tgaggttcat cacctcgatc ttccatttcc gggaatccgc
     1261 gtaccgggag tacatgcgga agaggtccgc cacgaagagg gccgcctcgt cgccgccggt
     1321 gcccgcccgg atctccatga tggtgttctt gtcgtccagg gggtcccggg ggatcaggcg
     1381 gtcccggagc cgggtttcca ggtccccgat ccgggcttcc agggtccgcg tctcgtcctg
     1441 ggccatggcc cgcatgtccg ggtccttctc gtcccggccc agggagcggg cgccctccag
     1501 gtccgacagg gcatgcttgt actcgtcgta gagcaccatg gtctccgaga gccgggcgtg
     1561 ttcccgcatg gtgtcgcggt aggcggcggg gtccttgacg aggtcgggat cggaaacccg
     1621 gacctccagc tcccggtgcc gctccgccag tgcctcgagc ttgtcgatca caggtcggcc
     1681 ttctccttga agtaggggca tccgtagatg acgatctcca gggactcgtc ggatttccca
     1741 tccagggagt ccgcgttggc tatgaggagg cgcttgatcc ggcagtcgtc ggtgaaggtg
     1801 cagatggggc aggcgccgtt cccgcggggg ttgatctcca gtttcatgga gatatggtat
     1861 aggaaattac gccactcgcg caagggagag gggtgggccg aggggtgccg atcccgctcg
     1921 tatccgcgtc ggaaaccgac aaggagccgc ccttgcctct gcccgcccgg ctcggctatc
     1981 atcccccatc atgctggatt accgcttcat caaggacaac ctggccgcgg tcaagaagaa
     2041 cgtcgcggac cgacacatgc aggccgaccc ggacctcgtg gtcgagctct tcgaccgccg
     2101 gggcgagctc ctccgccgcc tcgaggacga acgccgggcc cggaacgaca acgccgccgc
     2161 catgaagggc aagctcccgg aggaggagcg ccgccgcctc atcgaggagg gcaaggccct
     2221 caaggaccgg atcgcttccc tggaggcgga atacgcggag gcggaggcgc acctggacgc
     2281 cgagggccgc cgcatcccca acatggccca tccctccgcc ccggtcggga aggaggacaa
     2341 ggacaacctc gaggtcaagc gggtcggcaa gcccacggag ttctccttcg agcccaagga
     2401 ccacgtccag ctggggcagg acctcgatat tctggacttc gacaccgcca cccgggtgtc
     2461 cgggaccaag ttctactacc tcaagaacca gggcgtgatc ctggagctgg ccctggtccg
     2521 cttcgccctg gaccgcctcc agaagaaggg cttcaccccc ttcatcaccc ccgacgtggc
     2581 caagaccgag atcctggagg gcatcggctt caatccccgg ggcgcggagt ccaacgtcta
     2641 caccgtggag ggcgagggcg cctgcctcat cggcaccgcg gagatcaccc tggggggcta
     2701 ccactccggg caggtcctgg acaaggccgc cctgcccctc cgcctggccg gggtctccca
     2761 ctgcttccgc cgggaggccg gagccgcggg ccagttctcc aagggcctct accgggtcca
     2821 ccagttcacc aaggtggaga tgttcgccta cgtcctgccc gaggactcgg accgggtcca
     2881 cgaggagctc cggggcatcg aggaggagat cttctcggcc ctggagatcc ccttccgggt
     2941 cgtggacacc tgcaccgggg acctgggcgc cccggcctac cgcaagtggg acctggaggc
     3001 ctggatgccc ggccgcaacg gcggtgagtg gggggaggtc acctccacct cgaactgcac
     3061 ggactaccag tcccggcgcc tcaacgtccg gtacaaggac gacgacggca agaacaagta
     3121 cgtccacatg ctcaacggca cggcgatcgc ctgctcccgg gccatcatcg cgatcctgga
     3181 gaacttccag cgggccgacg gctcggtccg gatccccgcg gccttggtgc cctacacggg
     3241 gttcgaggag attcggagag cgtgagtacc gcgggggacc gccctaggcc gtccccccgg
     3301 tccgcctccg cccgccgtag gatcccacga ccgcccccgc catgatgagg gccagggctg
     3361 cgaccgtggc ccaggtcagg gtctttcccc ccgccacgac caggttgaag gtggacaaga
     3421 ggggcgtcag gtaggccagg gaaccgatga tccggggatc cccccgcttc agggccgcgt
     3481 cccaggtgta gaaggccagc cccatgggac cgagccccag gaagatcagg aagagggctt
     3541 cccggcccgt catcgggggc agacggacgc tccccgtcgc ggccaggtcc aggcccgccc
     3601 agagggccag ggagagggct cccgagatca ggcagaagag gcccaccgcc cccgtcgaga
     3661 aggggggaac tcgtttggtc aacagggaat aggtcgccca gatgaaggcc gccgccgcgg
     3721 ccatgaggtg ccccggaagt ccctcgggat tcaggctgaa ccgccctccc cccaccacca
     3781 ggaaggctcc cgccaggccc agcagggctc cgacggcgtg gcccgggcgc agctcgaaac
     3841 cccgcaggta caccggggag agcaccacga tgagcagggg ccagaggtag ttgatcaggt
     3901 tgacttccac ggccggggcc agccggaagg cggcgaacag tagggcgtgg tagccgaaga
     3961 tgcctcccac cccgaccgcc agggtggccg gggggatccg ccagtcccgg acccggaaaa
     4021 gcccggccag tccgccgatc cccagggccg ctcccacgga aaagaggggc ggaagagagg
     4081 cggtctggga cgtgaagagc gccagggtgc tccagacgag gatgctggcc aaggccagca
     4141 ggataggaac catgcttgtt cgcggacgcc cggtcgtcag aggctgctga cccgggagga
     4201 cagggccctg cgccagtcct gtttcttgcc ctcttcctcg aaggcgagcc ggcgcttcac
     4261 cgtgaaggcg ttctcccccc ggtccttgaa ctccacgacg gcgaaggctc cggacccgat
     4321 gtacacgagc ttctccccgg gagccaggga ctcgcggtcg gccatgcgct ggaaggcgca
     4381 gtcgaagtgg acggggcccc cggattccgg gtcggccaag gccgtggaga ggtcgtagat
     4441 gggtttctgg caataggcgc agtccagggg cggattcggt ttctgggact ccgcggcggg
     4501 agccgcgtac tccgcctccc gggacgcggc gggacggtcc gggcgatcct gccggccctt
     4561 cctcctcctg ccgaatctcc tgccgctacc gcgcctttcg tccgccatgc tgcaccccgt
     4621 atccgttcat gatctcggcg ctcagtacct gggcgatacg ctggatggcg ccggcgatgt
     4681 actcctcatc ttgcattttc ctgcgaagtt cttcaagtcg cctgggatcg tgcttgcgtt
     4741 cgccgggaca gctcatgggt gctccaggaa ggcctgcggg atgtgccggc agacggccga
     4801 gccgcggacc aggattacgt cgtatcgcgg gcgcatacgg ttatattctc gtttcagcgc
     4861 aaggaagtat ttagatgatt ctacgatatg ccgccgcttg gcgggcccga ggagccggtc
     4921 cgcggattcc ggaccgtagg cgtccacggt cttgacctcc acgaaggcca ggtcctcgcc
     4981 ccggagggcc acgatgtcca gctcccccgc gggacagcgc cagttgcggt ccacgatcgt
     5041 ccacccctcg gacagaaggt actccgccgc cgcggcctcc ccgagacggc ccttttcgac
     5101 gcgtcggatc ttcccctccc ggcccggtca gcccgaggcc acttccttgg gatccagggc
     5161 gcgggcgatg aagagggcct tttcggacag gaggtccacc tggtcgccct tgtcgtcctg
     5221 aaagctcttt ccgaactcgt ccaccaggat gcggagcttg gggcgcaggg gcgcgtccgg
     5281 gtgtgtctcc acgacccggg cgatggcggc gttgttgagg aggaccatgg atccgatggg
     5341 gtagatcccc atgctgcgga tgaacgcctt gaggatttcc gggtcgaagc gccgggaatt
     5401 gtccgagagg agggccttca tggccgcgta gccgatcatg gagtttcggt agggcttctc
     5461 gctgaccatg gcctcgaagg cgtcggcgac ggatacgatc cgggaaagga cgtcgatgtc
     5521 ctgcccggcc acccgccggg gatagccctg gccgtcccag cgctcgtggt gctgcagccc
     5581 gatgatcccg acgtcgtccg ggtacaggag ctccttggtg atgatcttgt aggtcaggag
     5641 ggggtgggcc cgcatcttct ggagctcctc ctcggaaaga tgccccttct tccggacgat
     5701 cgcctcgggg atcctcagca tccccgcgtc gtggagcaag gcccccgtgg ccaggtagga
     5761 cagccggtac ggagggagct tcagggtgac cccgatcacc accgagagga tggcggtgtt
     5821 caccccggcc cgggcgtaac tgtagccctg catgtccccg gacaggatgg ccgccaccgc
     5881 ccgaccctgc ttgtcccgga cgacccggag gacgtcctgg gagatctggt ccaccgtctt
     5941 gggctcgacc ctctccatcc ggcccaggcg gtcgtagacc gccttgagca tgtccaccat
     6001 gtccgtgtag gaccggtaca gttcccggtc cccgaggaag gcggcgggat tgaccccgtc
     6061 cgcccggggg ccgtccgggg aggaggatgc ggtcccggcg atctggctgc cctcggtgaa
     6121 gacgaaggcg acgccccatt tctggagcat gtccaggtcc ttctgcttca ccgccacgcc
     6181 cgcgggcacc aggaggttct ggtcgtcgat gaaaacgtcc tccgtgaagg acgcgcccgg
     6241 cttgatctga tccagggtga tcttcttttt catatccctt ccagcttcgc gatgaaaacg
     6301 taaacccggg ccacgatttc ccagtacgcc tcgggcacca tatcgccggg atccagggga
     6361 aagaggaatc ccgccaaggc ctcgtcggcc aggacgggta tgcccagttc ctccgccaga
     6421 tcccggatcc gctcggccga agccccggcg cctttcgcca ccaggaacgg ggcgggaagg
     6481 ctgtcgtcgt accggagggc tacggctttc ttcgtcctac ctgcaagtat cggccggatc
     6541 cggggggatt cttcatcaag catgatcgtc caccgccggg tactccgcat cccggttccc
     6601 ggtttccagg acctccacgc ggcagccgaa gtccgccagg ctccgctcca ggcccccgag
     6661 gccgccggcc gggccttcga gcccgctccc ggaacggtcc gcgaacccca gccggagggc
     6721 gttcccctct cctcggaggt cgaaggcgta cgagcccgtc cggcccggac cgtccacatg
     6781 tacgtccgcg gacaggacgg ccccggaacg gctcccccgg ggtaccagta tacgcagggt
     6841 accggaaaac gcaacgcctc cgcgctcgaa gcggtaggga acgtagatcc accgtccccc
     6901 cgggccggac cgggcgttga agacctgccg ggggtcgggc cgatctccgg tccggcccgc
     6961 cagggtcttg aggagacccg cgagggcttc cggactttca ggatcgtccc cccctgtacc
     7021 gggatactcc ttccagaccg ggccggaagc cggggcgccc gcgggtacgt cctcccccgg
     7081 tccggtgtcc ggccgctccc ctcggggagc gtcctgccgc tccccgccgc tcccgcccgc
     7141 ctcctcgcgg gatccgccgt cccggccctc ctgcgcttcc cgccggccgc cggggtcccg
     7201 tccgccgccc tcccctcgtc catccgcgaa ggacgcggcg tccacggcgt cggcgtaggc
     7261 cccgggctcc agccccttgg ccaaggcccg ggccaggagg gccgccctct cctcgtcggc
     7321 ttcctttttg agcaggacgg tgcgcaggcg ggcgatctgc cggggctcca ggggaaggcc
     7381 ctcggccatc agggcgcgca gggccaggat cgaggccgcg tccccgggga gccccgagga
     7441 gcgcaggaac tcggacgccc tcccttccac cgcgggcccg aggacccgga gggcccaagc
     7501 cccgtccggc aggcgttccg cccgggcgga atagaggctt ccgggcttga gggatgcggc
     7561 cacggccgcg tccaggcgga caccccctac caggacggtc catcgtccct ccgaggaggg
     7621 ggccagggcc ttgaaaacga tcgaggcgcc ctcccgcaac cccgggacct cgggtcccgg
     7681 agcgccggcg gcgcctcgaa tctgcaagga tcgggtcgag tccgaaccga tccgttccat
     7741 tcccggttat gcctgggccg gggcctcggc tgcggcgctt tccttcttgg gagccttccg
     7801 gcctccgaga agctccttga ccttggcctt cttgcccacc ttctcgcgga tatagtacag
     7861 cttggcgcgc cggggcttgc cgtagcgcac gatctcgagc ttctcgatcc tcggggaatt
     7921 gagggggaag acgcgctcca ccccgacgcc gtaggaattc ttgcggacca tgaaggaccg
     7981 gccgatcccc gcgttcttga tggccatcac gaggccctcg tagatctgga cgcgctcgtt
     8041 cttgccttcc acgatcttga aatggacgcg aacggtgtcg cccggcttga aatcggggac
     8101 ctcgcggagt tccgccgcct cgatcttctt aatcaggttc atcgattccc ccttcggctg
     8161 ctattcgctc gatcaatccg cgcaggtcgt ccgacagcat cgcgtccgag aggagctcgg
     8221 ggcgataggc gagcgtcttc tcgagccgct tcttcaggcg ccagcgcgtt atgtttgcgt
     8281 ggtgtcccga gaccaggacg tccgggactg ggatactatc ataaagggcc gggcgtgtat
     8341 actgggggta ctccaggagc cccccctcga agctctcctc cgccagggat tccccggtga
     8401 tgaccccgtc gatcagccgg tagaccgcgt ccaccaccgc cagggccgcg acctccccgg
     8461 aggacaggac gtagtccccg atggagatct cgtcggagac gtacttgtcc acgatccgct
     8521 ggtccacccc ctcgtaccgt ccgcagagga tcaccagctt cggctcccgg gccaagtccc
     8581 gggcgtaggc ctggttgaac aggcgccccc cgggggtcac gaagacggtg cggacgcccg
     8641 gggaccccgc ggcgtccagg gcccgtccca gggggcccgg gagcatgagc atcccggccc
     8701 ctccgccgta gacctcgtcg tcgcacttgt ggtgcttgtc cgtggcgtaa tcccggatgt
     8761 tcaccaggtc gtaggtcacg aggcctcgct ccaccgcccg tttcatgatg gagctctcga
     8821 agaaaccctt gaggatctcc gggaagaggg tgagcaccgt aagcttcatt ccaggatcca
     8881 gtccgccaga agctcgatcc ggcggccggc cgtatccacc tctccgatga attccttccg
     8941 gaacggcaca aggactgtcc gggcgggggt acggctaatc tccagcaggg gggcttcccc
     9001 gccttccagg acggcttcca cggtcccgac ggatgcgccc gcgaacacca ggtcgcagcc
     9061 cgcgaggtcc gcgatgtagt actccccggg ccccaggggg gcggcccggg accggggcac
     9121 caggatttcc cagcccgtcc acgcccgggc ctcctcgggg caggcgctgc ccgcgagccg
     9181 caggaggacg tccggcccgg agacgcggga ctcctccacc cggacggtcc tgcgctcgcc
     9241 ctcccgggtg agcagaatct cccccaggtc cgcgaagtgc gcaacctccc cggaatagct
     9301 ctggacgcgc aggaagccct ggaccccgtg gggggatcca aggcgcccta tggcgagaag
     9361 atcttgctct tgtccggctg acggattctg catggaacgg atgctcccgg aaccttagga
     9421 gcgcgccttg cgcgtcccta agggccttaa tccaggattt ccaggaccac ccgcttggcg
     9481 gaatgcgagg cggaggcgga gaggatggtc cggagggcct tggcgatccg gccgtgcttg
     9541 ccgatcacct tgccgacatc cccctcggcc acccggagct ccaggaggac ggacttgtcc
     9601 ccgtcgacgg cggtcacgga gaccgcggag ggatcgtcca cgagggattt tgcgatgtat
     9661 tcaacgaggt cgcgttccac ggttcgctcc ttggatccgc atcgggcggc gccggagcgc
     9721 cgctcagccg agtcggatgt tcttcttgtt gaagaggctg cgcaccgtgt cggacgggag
     9781 ggcgcccttg gcgagccagg ctcgggcctt ggcctcgtcg atgaccacct gcctgtcctc
     9841 ggactcgatc ggatgataga tgccgatctc ctcgagggtg gagccctcgc gggccagccg
     9901 ggagtccatg accacgatgc ggtaataagg gcgcttcttg gcgccgaatt tcttgagccg
     9961 gatcttgacg ctcaacggtc ttcctcctac ctgacatgac gacgcgatcg tcggttcgat
    10021 tgcggaatga tcggatcccg gcggggtgaa cggagccgga agcccgcgaa agaccgttat
    10081 acaggaaggg agggaaagag tcaatgtgcg ccgagccgcg tcgcgtcatc catgacgatc
    10141 cagaatcgca tacgcgattc tggccctccc cgcaggcgcc catacggacc ttcggcccgt
    10201 ttcccggtcg gaaaggatcg aagcatgcgc taccgcgcat ggcgggatcc ttgggcgccc
    10261 tgacgcggtc ttccggcgac gatgcctcga ctagaaacat ctccgattcc catcgaagct
    10321 gaagcgaggc ttcgtcgagt ttccagcttc ccgttcacta ttcactattc cccattcacg
    10381 gtatcctccc cccatgcccg cccgcccctc ccccctgccg ccggacgccc tcctcctggc
    10441 ccccatggtc ggactgtccc accggcctct ccgggagctc gtcctgggct tcgggggctg
    10501 cgacctggcc tacacggaga tgacgagcgc ggcgggctac ctttccgggg ccccctacga
    10561 ccggtacttc ctggacacgg gtccggagcc gggacgcgcg gtcctgcagt tctacgccgt
    10621 tgacgccccg cgcatggtcc aggccgcccg caaggccgcc cgggagatcc ccgccgcggg
    10681 tctggacctg aacttcggct gctccgcccc ccacatcgag aaggccggag ggggcgtctc
    10741 ctggatgaag gaccccgggg cggcccggga cctggtagcc cgggtccggg acgcccgccc
    10801 ggaaggcggc atttccgcaa agcttcgggt ggggtacgac gaagactacg gccgtctgcg
    10861 ggacttctgc ggcggcctgg ccgaggccgg ggcggactgg ctggtcctgc acccccgcct
    10921 gcggagcgaa aagctccgac ggacgggtcg ctgggactat gtccgcaggc tcggggagga
    10981 cctggatatc cccgtcgtgg gcaacgggga catacggggc cccgaggacc atgcggcccg
    11041 ggtccgggaa tgccgccccg ccgggatcat gctgggccgg gaggccgtcc gacggccctg
    11101 ggtcttcgcc ctcatccggg gactggaacg ggatcccggt ttccagatta ccgtcgacat
    11161 agccgggacg gcggagaccg ctctccggct catggaggag catcagcccc cggagttcca
    11221 catctcccgg gcccggaggt tcttctatta ctactgcgac aacctctcct tcgcccacca
    11281 tatccggtgg aagatccaga acgccccgga cctgaaggcc atacggcaag ccctggcgga
    11341 gtacttcgag gaagtgccgg gtgacaggag tcaggtaaca ggagccaggc cacaggagcc
    11401 aggcgaccga gggacctcaa gatgacggat gcatggacca atctacggtc gtctggttag
    11461 taccctgagt cctgaatttc cttgctcact tttacattat tcgttattat gtaattcatc
    11521 ctatgtcccg aagggggaag accacatggc cgagaccctt gcccagcttt tcctggaaaa
    11581 agtccgcgat taccccgaat tgaacgccca gtacagcaag aacggcggaa acgaattcaa
    11641 gcccacgacg taccggcagc tcctagagga ggtcgcatcc ttcgcggagg cccttaccga
    11701 atccggggtc aagcgcggag accgggtcgg gctgatctcc gacaaccgga aggagtggct
    11761 gatcacggac ctcgccctcc tcgggctggg ggcggcggac gtgccccggg gctgcgatgc
    11821 cacggaccag gaagtccggc acatcctttc ctggagcgag tgccgcctga cggtcgtgga
    11881 gaacgacaag cagctcaagc gggtgatcga tcttcgggga cagatacccc tcctggagaa
    11941 ggtcatcgtc tacgaatccg tgcccgagga gctcctggcg gccgccaaga aggccaagct
    12001 ggaggtccgc acctacgccc aggccatgga ggaaggcaaa aaactccgcg ccgagcgccc
    12061 gggccggtac gagaaggaaa ccgccgcggg caagcgggac gagaccgcca cgctcatcta
    12121 cacctccggc accaccgggg agcccaaggg ggtcatgctg acccacggca acttcctgta
    12181 ccagacggac tacatcctga ccatcatcga catcaagccc gggcacatct tcctctcggt
    12241 cctgccgatc tggcactcct tcgagcgcat cgcccagtac atgatcctgg ccgccggcgc
    12301 gggggtcgcc tactccaagc ccatcggggc catcatgctg gcggacctcc aggcgatcaa
    12361 accccagtgg ctggcctccg tgccgcggat ctgggagtcc gtcaaggacg gaatctaccg
//
LOCUS       DAOTEI010000100        12284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3616_length_12284_cov_4.968845, whole genome shotgun sequence.
ACCESSION   DAOTEI010000100 DAOTEI010000000
VERSION     DAOTEI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3616_length_12284_cov_4.968845"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="P5313_10415"
     CDS             <1..1255
                     /locus_tag="P5313_10415"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRY80819.1"
                     /translation="AGSELRACVDRMAADGLRVIAVASSRIPAGGLPEGQHDFPFELQ
                     GLMGLQDPIRAQVPDAVALCRRAGIRVLMITGDYPGTASNIARKAGIDNPGDVLTGPE
                     IETLDAGELRVRLARTNVVARAVPEQKLRIVEALKSNGEVVAMTGDGVNDAPALKAAD
                     IGIAMGGRGTDVARESADLVLLDDDFASIVQAVKTGRRIYDNLRKAITFILAVHLPIA
                     GMALLPVLLGWPLALLPVHILFLELIIDPACSIIFEMEDEEEDVMDRPPRKTTERLFG
                     RNLVAMAVVQGLAVFTLVAAIYGWSLLSGFGEGEARALSFVGLVFGNIGMIISNRSRS
                     QGLFRILARRNPAQTWVVGGALAFLAVAVFVPAVNSIFGFAPVHLWQVLFSLGSGVAA
                     VAAAESVKLPGLIRARRIGKSEASN"
     gene            1252..2217
                     /locus_tag="P5313_10420"
     CDS             1252..2217
                     /locus_tag="P5313_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY80820.1"
                     /translation="MKRGTDEKTFRVAVFGAGVIGSLYAGRLAEAGCEVAILARGDRA
                     DKIRSDGLRLEDERSGRRTQVRVAAPDSLREFRPDAVFVAVRKDQADEAAREIARDDP
                     GGLVVPMVNAADGHPEFRSRFPEARLAFAFPGAGGTRLPDGTVLYTLVPGIVQATTLG
                     PSGPGTDRVTLLRLLQAIRKAGFPAALERDMDAWQKTHVALVSPIADALYAKAGDHRA
                     LARDTRTLRLMAAAVREGFRALRASGITIRPARLGILAVLPASLVAAAFAPAVGSSWG
                     ETVIARHANAARAEMETLAGGLRALVDASGVEAPSLRELWARVRG"
     gene            2316..3209
                     /gene="rlmN"
                     /locus_tag="P5313_10425"
     CDS             2316..3209
                     /gene="rlmN"
                     /locus_tag="P5313_10425"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRY80821.1"
                     /translation="MAAASTALRVLDTASEPGSAGTTVKYRLGLADGLAVESVAVPMH
                     GGTYTVCVSSQVGCARACAFCETGTAGLSRNLTAAEIVGQVTAASEALGVRFRNVVFM
                     GMGEPLDNPEGVFGALETLRDPRGLGYSQERITVCTAGHVEGIRRLRALGLKRLNLSV
                     SLASARDELRDRLMPINRTWPLGELAEALAAYPMRRNFVLGVNYCLLPGLNDASEDLD
                     AAAAFVGRLGRALVNVIPYNPGRHPICRAPSEPEIDAFLAGLAARGVPVRRRVEKGRS
                     LQAACGQLGGGSARPFASQAS"
     gene            3259..4110
                     /locus_tag="P5313_10430"
     CDS             3259..4110
                     /locus_tag="P5313_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRY80822.1"
                     /translation="MRYRTIRKIVTGAPAVDGAGVRLVRVLGHRDTEDFDPFLLLDAF
                     DSENPDDYVRGFPWHPHRGIETVTFLVEGDIEHGDSLGNRGSILDGGCQWMTAGSGIV
                     HQEMPRASPRMLGLQLWLNMPASRKMSPPAYGDLPKERIPVVEEGGTRIAVVGGSFRG
                     TPGAKRGDYVDATLLDLALEPGATLEIPVPAGDTAFVYIMAGSVSFDAPEAGTFQDRR
                     RAVLLADGDAVRLAAGKAGPVRCVFCSGAPLGEPIAWGGPIVMNTRAELDRAFRELDE
                     GTFIRKG"
     gene            complement(4127..5269)
                     /locus_tag="P5313_10435"
     CDS             complement(4127..5269)
                     /locus_tag="P5313_10435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY80823.1"
                     /translation="MLQTLAYYLAFLIIGLATASFGPGLPEFARNTGASLAALSNLFL
                     FHRVGYIAGSLGGGRLLDRTRGNRLTGLVLGLIALGLVSVSRAAVLPVLLGSILVLGL
                     SQGTAETGANTGVVRLHGDRAGPYMNGLHLSFGVGALLSPLLLSASRAGTGSFRAAYA
                     GFAALAALAGLWILRLPEPPARSRGAGEAGRNPLSFWMIAGILFFTVAGEAGFANWAF
                     SYARKAGLASETGAGLLTSAYWTSLTLGRLIGIALVQRLGPLRLLTLIVPGALAGSAV
                     FLAFPDAAWAVWTASVLLGLFQASAVPASFTLAGDLGILSGSTAGLFGAVSSLGGMAL
                     PWLIGRYFDSTGGAVFPVLIAASQAAALACLVGIRAVRGKPDAGRG"
     gene            complement(5644..5716)
                     /locus_tag="P5313_10440"
     tRNA            complement(5644..5716)
                     /locus_tag="P5313_10440"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5681..5683),aa:Pro,seq:cgg)
     gene            complement(5749..5823)
                     /locus_tag="P5313_10445"
     tRNA            complement(5749..5823)
                     /locus_tag="P5313_10445"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5786..5788),aa:Asp,seq:gtc)
     gene            complement(5872..7428)
                     /locus_tag="P5313_10450"
     CDS             complement(5872..7428)
                     /locus_tag="P5313_10450"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HRY80824.1"
                     /translation="MSEPQKKRPGSGPRKNDPRSDRRPSSPGTRPFRGGPERESPSPG
                     RPGPRRPGPPRGAPFRSGDRAPGTREPNRPEAPVHARHEDGDPITAYLASQEGVRTGG
                     GMKARFVAPFGAPKNAGSDLEAFLAAVSETFPVQESRRRYLKEDVLTLWRDLTSERSL
                     RFADYLGKPSALAAYVRYFMAWNVVRLVPVLAGLPLDPPEGATILDIGSGPLTLPIAL
                     WIARPDLRDRGLRILCTDRVRKPMESGAALLDALRLKTGGPVLGDGAWRVETRHAVFP
                     DTGGPGKADVVCSANAFTEFFWSASARRDSNLGERAAVLLSDLAAACKPGGLLFIAEP
                     GEPRSGAMLSALREAALLSGLRVESPCPHARACPMPGAFGGLGDRVDGKARSALPPVH
                     SPKWRPKMPWCHFPVPLDTAPSALTRFSEEVGLPKERLTVSWLALRKPAVGDRAPEAE
                     QKGPLRTRLVSDPIRLPGGWDGRYACSDLGYTLVVGAAAREGPGSLLDLDRPARISRD
                     EKSGAVIAGP"
     gene            complement(7492..8514)
                     /gene="tdh"
                     /locus_tag="P5313_10455"
     CDS             complement(7492..8514)
                     /gene="tdh"
                     /locus_tag="P5313_10455"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRY80825.1"
                     /translation="MKALVKKYSKPGLWMEDVPMPKVGDNDLLIKVKKTAICGTDVHI
                     WKWDAWAQKTIAIGQTVGHEFVGEIVEMGRAVEGYKVGERVSAEGHIVCGVCRACRAG
                     KHHLCPNTRGIGVNRDGCFAEYVSVPAYNALHIHSSVPDEIASIFDPFGNATHTALSF
                     DMVGEDVLITGAGPIGCMAAAIARHVGARNVVVTDVNDYRLDLARKLGASRTVNVARE
                     RLMDVQKELGMVGGFDVGLEMSGSPAAFEEMLANMYNGGRIALLGLLPSGTGIDWEKV
                     IFKGLFIKGIYGREMFETWYKMQAMVLSGLDISPVITHRYSFGDFEKGFADMVSGQSG
                     KVVLSF"
     gene            8778..9920
                     /locus_tag="P5313_10460"
     CDS             8778..9920
                     /locus_tag="P5313_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY80826.1"
                     /translation="MPDRLKNFNSGRSNFYLLGFIALILAGAVLKITAPVLLPFTIAV
                     LLSFVLEPLVFALEKLRIPRIIGILLSILFIGAGLYLIGVILFSSGRTILTLYPKYER
                     RFSEIYSRVAEVFSLPYDEHLTFFQNLWGQLGLRVRVQGLAFQLSEAFLGFLKDAVMV
                     LLFVAFLLLEISHFRDKIELAFEDKLSGRIKKAASDVVNQVARYLTVKFLISLATGVL
                     VTLGLSLLGMDFPVVWGVVSFILNFIPNIGSVAAGAGVGVFALVQFWPEPGPIAGSLA
                     IMVGVNMVLGNVLEPKVQGDNLGLSPFVILVSMLAWGWLWGFAGLILAVPMTVIVKIV
                     CENVPMLEPASIILGSFRAAVRKKGSEPEHAAPATPFDPPGASQGS"
     gene            9942..11234
                     /locus_tag="P5313_10465"
     CDS             9942..11234
                     /locus_tag="P5313_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY80827.1"
                     /translation="MTKTQKTLLLVLMAASMVALNADTQVMAPMLVTIEAEFGVDDSA
                     VGAMMALFTVVGAVVSLFWGYFADKASRKKLFVFSVALGEIPCALTALSPSWEVFFAL
                     RILTGIGVGAAFPLVFSILGDIYNEKERPMASAILTTSFGLGAIAGPVLGGFVGQAAG
                     WRLPFFLAAVPNIPLLLAFWFLVPEPRAAASEEATRELVEAGILYPKHIRVQDYARLG
                     KVPTNVYLFIQGIAGTIPWGAFFFLNKFLETSKGLSVGQATAVYMVFGAGMVAGNLAG
                     GKLGELVFRRSAKDLPLFCGVTTFLGCLGTLGVVTLRGSIPLLSAAGFLAAFFAAMTG
                     PNMRTMLLDVNPPEDRGPIFSIFNLTDSLGTGLGRWVAGMLSVAVGLSGALTISASFW
                     VICGIVLVFTARIFPKDIGILNERMVQTAREMRGQSST"
     gene            complement(11915..>12284)
                     /locus_tag="P5313_10470"
     CDS             complement(11915..>12284)
                     /locus_tag="P5313_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY80828.1"
                     /translation="DCLYLILSGSARVTVEENGRDAYICTLGAGDVFGEAGLFMNLKR
                     TANVAAADDGMTVLRVKRSDFMRFIREHPAAGIKVLFMIIYSLLAKLREANLELAFER
                     REDIAQDDIDDLLRDYLEQK"
BASE COUNT         1932 a         4041 c         4193 g         2118 t
ORIGIN      
        1 ggccggctcc gaattgcgcg cctgcgtgga ccgcatggcc gccgacgggc tccgggtcat
       61 cgccgtggcc tcgtcccgga tccccgctgg ggggttgccg gaaggacagc acgattttcc
      121 gttcgaactc caggggctca tggggctaca ggacccgatc cgggcccagg tcccagacgc
      181 ggtagcgctc tgccgccggg ccggcatacg ggtgctcatg atcaccggcg actatcccgg
      241 cacggcctcg aacatcgccc ggaaggccgg aatcgacaac cccggggacg tgctcaccgg
      301 ccccgagatc gaaaccctgg atgccgggga gctccgggtg cggttggccc ggaccaatgt
      361 tgtcgcccgg gccgtgcccg agcagaagct ccgtatcgtc gaggcgttga agtccaacgg
      421 agaggtggtc gccatgaccg gggacggggt caatgacgcc cccgcgctca aggctgcgga
      481 catcggcatc gccatgggcg ggcgcggaac ggacgtggcc cgtgaatccg ccgacctcgt
      541 ccttctggac gacgatttcg cttcgatcgt gcaggcggtc aagacgggcc gcaggatcta
      601 cgacaacctg cgcaaggcca tcactttcat actggcggtg caccttccca tcgcggggat
      661 ggccctcctc cccgtgctgc tcgggtggcc cttggcgctc ctgccggtcc acatcctgtt
      721 cctggaactg atcatcgatc cggcctgctc gatcatcttc gagatggagg acgaggagga
      781 ggacgtcatg gaccgtcctc cccgtaagac cacggagcgc ctgttcgggc ggaatctcgt
      841 ggcgatggcc gtagtccagg gattggcggt cttcaccctg gtggcggcca tctacgggtg
      901 gtccctgctt tcgggattcg gggaggggga ggcgagggcg ctctccttcg tggggctggt
      961 gttcggcaac atcgggatga tcatatccaa ccggtcccgg agccaaggct tgttccggat
     1021 cctcgcccgg cgcaatccgg cgcagacctg ggtggtggga ggtgccctcg ccttcctcgc
     1081 ggtcgccgtg ttcgtgcccg cggtcaacag cattttcggg ttcgcgcccg tgcatctctg
     1141 gcaggtgctg ttttccctgg gctccggagt ggcggccgtc gccgcggccg agagcgtgaa
     1201 gctgcccggt ctgatccggg cccgcaggat cgggaaatcg gaggcgtcca attgaagcgt
     1261 ggcacagatg agaaaacctt tcgcgtcgcg gttttcggcg cgggcgtcat aggcagcctg
     1321 tacgccgggc ggctcgcgga ggcgggctgc gaggtcgcga tcctggcccg gggcgaccgg
     1381 gcggataaga tacgaagcga cggtctccgg ctggaggacg agcgttcggg acgccgtacc
     1441 caagtacggg tggccgcccc ggattccctt cgggagttcc gaccggacgc ggtgttcgtg
     1501 gcggtgcgca aagaccaggc cgatgaggct gcccgggaaa tcgcccggga cgatcccgga
     1561 ggcctcgtgg tacccatggt gaacgccgcg gacggccacc ccgagttccg gtcccggttt
     1621 ccggaagccc ggctggcctt cgccttcccc ggcgccgggg ggacccggct cccggacgga
     1681 accgtgctgt acactctcgt gcccggcatc gtgcaggcca ccaccctggg gccctcaggg
     1741 cccggaacgg accgggtgac cctccttcgg ctcctgcagg ctatccgcaa ggcggggttt
     1801 cccgccgcac tggagcggga catggacgcc tggcagaaga cccatgtggc cctggtgagc
     1861 cccatagcgg acgccctgta cgccaaggcc ggcgaccacc gggctctggc gcgggacacc
     1921 cggacgctcc ggctcatggc cgccgcggtc cgggagggat tccgggccct gcgggcctcc
     1981 gggatcacga tccgacccgc ccgcctgggc atcctggccg tcctgcccgc ctccctcgtc
     2041 gcggcggcct tcgccccggc ggtcggctcc tcctgggggg agaccgtgat cgcccggcac
     2101 gcgaacgccg cccgcgcgga gatggaaacg ctggccgggg ggctccgggc cctcgtggac
     2161 gcatcggggg tcgaggcccc gagcctgcgg gagctctggg cccgggtccg gggctgaagg
     2221 ccgggtcggt ccgtaccggg ggcggttcct ccgccctcgg cgtgcgagct ctgtccccgg
     2281 ctgacctttt cccggccccc gcgtatacta ccggcatggc cgccgcatcg accgccttgc
     2341 gtgtcctcga caccgcctcg gagccgggct ccgcgggtac gacggtgaag taccgtctcg
     2401 gcctggcgga cgggctcgcg gtcgagtccg tcgcggtgcc gatgcatgga ggaacctata
     2461 ccgtctgcgt ctcctcccag gtcggctgcg cccgggcctg cgccttctgc gagaccggga
     2521 cggcgggcct ttcgcggaat ctcacggccg ccgagatagt cggccaggtc acggccgcgt
     2581 cggaggccct gggcgtccgc ttccggaacg tcgtgttcat gggcatgggc gagcccctgg
     2641 acaatcccga gggggtcttc ggcgccctcg agacgctccg ggaccctcgg ggcctgggat
     2701 actcccagga gcgcatcacg gtctgcaccg cggggcatgt ggaaggcatc cgccgactgc
     2761 gtgccctcgg cctcaagcgt ctcaacctct ccgtgagcct cgcctcggcc cgggacgagc
     2821 tgcgcgaccg gctcatgccc atcaaccgga cctggcccct cggggagctg gcggaggccc
     2881 tggcggccta cccgatgcgg aggaatttcg tcctgggcgt caactactgc ctcctccccg
     2941 gcctcaacga cgcttcggaa gacctggacg ccgcggcggc cttcgtcggg cggctcggcc
     3001 gggccctcgt gaacgtgatc ccctacaatc cgggccggca tcccatctgc cgggccccct
     3061 cggagcccga gatcgacgcc ttcctggcag gactcgccgc ccggggtgtt ccggtacggc
     3121 gacgagtcga gaagggacgg tccctccaag ccgcctgcgg ccagctgggg ggcgggtcgg
     3181 cccgcccgtt cgcatcccaa gcttcgtgac actatagttg aggtgcgccc ccgcgacggg
     3241 gacgcgggga ggcttgccgt gaggtaccgt accatccgca agatcgtgac cggcgcgccc
     3301 gccgtggacg gggcgggggt ccggctggtc cgcgtcctgg gccaccggga caccgaggat
     3361 ttcgatccct tcctgctcct ggacgccttc gactcggaga accccgacga ctacgtccgg
     3421 ggcttcccct ggcatcccca ccgggggatc gagaccgtca ccttcctggt ggagggggac
     3481 atcgaacacg gggacagcct gggcaaccgg gggagcatcc tggacggggg ctgccagtgg
     3541 atgaccgccg gaagcgggat cgtccaccag gagatgcccc gggcatcccc gcggatgctg
     3601 ggcctccagc tctggctcaa catgcccgcc tcccgcaaga tgtccccgcc cgcctacggc
     3661 gacctgccca aggagaggat ccccgtggtc gaggaaggcg ggacccggat cgccgtggtg
     3721 ggcggctcct tccggggaac cccgggcgcc aagcgggggg actacgtgga cgcgactctc
     3781 ctggacctcg ccctggagcc cggggccacc ctcgagatcc ccgtacccgc aggggacacc
     3841 gccttcgtgt acatcatggc gggctcggtg tccttcgacg cgccggaagc cgggaccttc
     3901 caggaccgtc gccgggcggt cctcctcgcg gacggggacg cggtgcggct cgcggccggg
     3961 aaggccgggc ccgtccgctg cgtgttctgt tccggcgcgc ctctgggcga gccgatcgcc
     4021 tggggaggcc ccatcgtcat gaacacccgg gccgagttgg accgggcatt ccgggagctg
     4081 gacgagggga ccttcatccg gaaggggtga gggggcgacc gtaccctcag ccgcgtcccg
     4141 cgtccggctt tccccggacc gcccggatgc ccaccaggca ggccagggcg gccgcctggc
     4201 tggcggcgat caggaccgga aagaccgccc cgccggtgga atcaaaatac cttccgatca
     4261 gccagggtaa ggccatcccc cccagggagc tcacggcccc gaagagtcct gccgtcgagc
     4321 ccgacaggat ccccaggtcc ccggccaggg tgaaggacgc gggcacggcg gaagcctgga
     4381 agagacccag taggacggag gccgtccaga cagcccaggc ggcatccggg aaggccagga
     4441 ataccgcgga cccggccagg gcccccggga cgatcagggt cagcagccgg agcgggccga
     4501 gccgctgcac aagggcgatc ccgataagcc gccccagggt cagggaggtc cagtaggcgg
     4561 aggtcaggag ccccgccccc gtctcggagg ccaggccggc cttccgggcg tagctgaagg
     4621 cccagttcgc gaagcccgcc tcccccgcga cggtgaagaa caaaattccg gcgatcatcc
     4681 agaatgaaag cgggttccgg ccggcctccc ctgcgccccg ggagcgggcc gggggttcgg
     4741 gcaggcggag gatccagagt cccgccaggg cggccagggc cgcgaagccg gcgtaggccg
     4801 cccggaagga tcccgtcccg gcccgggacg cggacagaag gagaggggag agcagggcgc
     4861 cgaccccgaa ggacaggtgg aggccgttca tgtacggtcc ggctctgtcc ccgtggaggc
     4921 ggacgacccc cgtgttcgcc cccgtctccg ccgtgccctg ggacaggccc aggaccagga
     4981 tgctccccag gaggaccggt aggacggcgg cccgggagac ggagacgagg cccagggcga
     5041 tgagccccag gaccaatccg gtcaggcggt tgccccgggt ccggtccagg agccggccgc
     5101 cccccaggga cccggctatg tagccgaccc ggtggaagag gaataggttc gacagggcgg
     5161 ccaagctggc gcccgtgttc cgggcgaact cgggcaatcc ggggccgaag gacgcggtgg
     5221 ccaagccgat gatcaggaag gccaggtagt acgctagggt ctggagcatg gggagagtat
     5281 ggcggagcgg gcgtcgaggt gcaatccaga ataagggtca cctcgcccgg atttctcgcc
     5341 ttgccagcgt cggtcgggtg cgctactcgg gggaccgacg cggaccgagg ccgcgcgagg
     5401 atcgtttcct agagtaaacc gcgcggagcg cggatttggt ctccacagga gccgtcctgg
     5461 ctcctcgttt cgggccggct ccgcgcctct cggcgcatcc ctgcgcctca ggctcgccta
     5521 cgctcgccac ggcgctccgc ttcaaatcca ctcagcaaaa atgggctgag tggacgccac
     5581 gaagcggcga ccaccttgag aaaagtgaag agccgcgtct ttcgacgcgg ctctgatcaa
     5641 agatcgggct gagtggattt gaaccaccga catcttggtc ccgaaccaag cgcgctagcc
     5701 cctgcgctac agcccgcaga aagaaaaatc ccgccgcgag gcgggatacg ggagcgacgg
     5761 ggcttgaacc cgcgatctcc ggcttgacag gccggcgcga taaaccagct tcgctacgcc
     5821 cccagcgtca tcgctgacag gagggaacta tagcgatcgg gccttcggat gtcaagggcc
     5881 cgctataacc gccccggact tctcgtcccg ggatatccgg gcgggccggt cgagatcgag
     5941 cagacttccg ggcccttccc gggccgcggc gcccacgacc agcgtatagc ccaggtcgga
     6001 acaggcgtag cggccgtccc agcctccggg gaggcggatg gggtccgaga ccaggcgggt
     6061 gcggagggga cccttctgct ccgcctccgg agctctgtcc ccgacggcgg gcttgcggag
     6121 ggccaaccac gacacggtca gccgctcctt gggcagcccc acctcctcgg agaaccgcgt
     6181 cagggctgaa ggagccgtgt cgaggggcac cgggaagtgg caccagggca tcttggggcg
     6241 ccacttcggc gagtggaccg ggggcagagc cgagcgggcc ttgccgtcca cccggtctcc
     6301 caggccgccg aaggcccccg gcatggggca ggcccgggcg tgggggcagg gggactcgac
     6361 ccgcagtccc gagaggaggg ccgcctcccg gagcgcggag agcatggccc cggaccgggg
     6421 ttccccgggc tcggcgatga acaggaggcc gccgggcttg caggcggcgg ccaggtcgga
     6481 aagcagaacc gcggctcgtt ctcccaagtt gctgtcccgg cgggcggatg cgctccagaa
     6541 gaactcggtg aaggcgttcg cggagcacac gacgtccgcc ttgccgggac cgcctgtgtc
     6601 cgggaagacc gcgtgccggg tctccaccct ccaggctccg tcccccagga cgggcccccc
     6661 ggtcttgagc cggagggcgt ccaggagggc cgcgccggat tccataggct tccggacccg
     6721 gtccgtgcag aggatgcgga ggccccggtc ccggaggtcc ggacgggcta tccacagggc
     6781 gatgggcagg gtgagagggc cggagccgat gtccaggatc gtcgcgcctt cgggaggatc
     6841 gaggggcagg ccggccagga cgggaaccag gcgcaccacg ttccaggcca tgaagtaccg
     6901 cacgtaggcg gccagggcgg aaggtttccc gaggtagtcc gcgaagcgga ggctccgctc
     6961 gctggtgagg tctcgccaga gggttagcac gtcctccttg aggtaccgtc tgcgggattc
     7021 ctggaccggg aaggtctcgg agaccgcggc caggaaggct tcgaggtcgg atccggcgtt
     7081 cttgggcgcc ccgaaggggg ccacgaagcg ggctttcatc ccgcccccgg tccgtacgcc
     7141 ttcctgggac gccaggtatg cggttatcgg gtctccgtcc tcgtggcggg catggacggg
     7201 ggcttcgggg cggttcggct cccgcgtgcc gggagctctg tccccgctcc ggaatggcgc
     7261 ccctctcggg ggtccggggc ggcgcggacc gggccgcccc ggggacgggg attcccgttc
     7321 ggggccgccc cggaagggac gggtaccggg ggaggagggc ctgcggtcgg accgggggtc
     7381 attcttccgg ggcccggagc cgggccgctt cttctggggt tcgctcacgg tgcgagtatg
     7441 ccgggccgcg gggttctgca caagaccgtc ccgcggccga cgccgaaccg cctagaacga
     7501 gaggaccacc ttgccggact gtccggatac catgtccgcg aagcccttct cgaagtcgcc
     7561 gaaggagtac cgatgggtta tgaccgggct gatgtccagg ccggacagga ccatagcctg
     7621 catcttgtac caggtctcga acatttcccg gccgtagatc cccttgatga acagcccctt
     7681 gaagatgacc ttctcccagt ctatccccgt gccgctcggc aggagaccca ggagggcgat
     7741 gcggccgccg ttgtacatgt tcgccagcat ctcctcgaag gccgcggggg atccggacat
     7801 ctccaaaccc acgtcgaagc cgcctaccat ccccaactct ttctggacgt ccatcaggcg
     7861 ttcccgggct acgttgacgg tccggctggc tccaagcttc cgggccaggt ccagacggta
     7921 gtcgtttaca tccgtgacca ccacgttccg ggcacccacg tgccgggcga tggcggcggc
     7981 catgcagccg atcgggcccg ctccggtgat gaggacgtcc tccccgacca tgtcgaagga
     8041 cagggccgtg tgggtggcgt tcccgaaggg gtcgaagatg gaggcgatct cgtcggggac
     8101 agagctgtgg atgtggaggg cgttgtaggc cggtacggac acgtactccg cgaagcaccc
     8161 gtcccggttg acgcctatcc cgcgggtgtt cggacagagg tggtgcttcc cggcacggca
     8221 agcccggcag accccgcaga cgatgtggcc ctccgcggac acccgctcgc cgactttgta
     8281 gccctccacc gcgcggccca tttcaacgat ctcgcccacg aactcgtggc ccaccgtttg
     8341 tccgatcgcg atggtcttct gggcccaggc gtcccatttc catatatgga catcggttcc
     8401 gcagatcgcc gttttcttga ccttgatcaa caggtcgttg tcccccacct tgggcatggg
     8461 gacgtcctcc atccagagtc cgggcttcga gtatttcttc acgagggctt tcatgtcgtc
     8521 tccttgggct tgccgggcag ctttccgcgg ggggatcggg gcggctccgg ctcggcgaac
     8581 agtagtatac aacccttttg gacggccggg aagctctgtc cccggggagt tctgtccgca
     8641 ggatgttttg cccccgggaa gctctgtccc cggatgctgg acgcagcgtt ggaagcttga
     8701 cctttcgggg tatgccgagc catgctggtg ccgccggcgc gaggtactgc gactccggcg
     8761 gacatcaagg ggggggcatg cccgatcgct tgaagaactt caattccgga cggtccaatt
     8821 tctaccttct gggattcatc gccctgatcc ttgcgggagc ggtcctcaag atcacggccc
     8881 cggtgctcct tcccttcacg atagccgttc tcctttcctt cgtactggag ccgctggtct
     8941 ttgcgctgga gaagctccgg attccccgca taataggaat cctgctttcc atattattta
     9001 tcggagccgg actgtacctc atcggcgtca tccttttttc ctcgggccgg accatcctga
     9061 ccctctaccc gaagtatgag cggaggttct cggagatcta ttcccgggtc gccgaggtgt
     9121 tcagcctgcc ctacgacgag cacctgacct ttttccagaa cctctggggc cagctaggcc
     9181 tccgggtccg ggttcaaggc ctggccttcc agctttccga ggctttcctg ggctttctca
     9241 aggatgccgt catggtcctg ctcttcgtgg ccttcctgct cctggagatc tcccatttcc
     9301 gggacaagat cgagctggcc ttcgaggaca agctttcggg caggatcaag aaggcagcct
     9361 cggatgtggt gaaccaggtg gcccggtacc tgaccgtgaa gttcctgatt tccctggcca
     9421 ccggtgtcct cgtgaccctg ggtctgtccc tcctggggat ggacttcccc gtagtatggg
     9481 gggtcgtatc gttcatccta aacttcatcc ctaacattgg cagtgtggcg gccggtgcgg
     9541 gggtgggcgt cttcgccctg gtccaatttt ggccggagcc gggacccatc gcgggttctc
     9601 tggccatcat ggtgggcgtc aacatggtcc tggggaacgt cctggagccc aaggtccagg
     9661 gagacaacct gggcctctcc cccttcgtga tcctggtgtc catgctggcc tggggctggc
     9721 tctggggctt cgcgggcctt attctggcgg tgcccatgac cgtgatcgtg aagatcgtct
     9781 gcgagaacgt cccgatgcta gaaccggctt ccatcatcct gggatccttc cgggctgcgg
     9841 tccgcaagaa ggggagcgaa ccggaacatg cggcaccggc gactccgttt gacccgcccg
     9901 gagcttcaca aggttcgtaa caccggcaga aaggagcgcg catgaccaag acccaaaaga
     9961 ctctcctact cgtccttatg gccgcgtcca tggtggccct gaacgcggat acccaggtca
    10021 tggcccccat gctggtcacc atcgaagccg agttcggggt ggacgactcc gccgtgggcg
    10081 ccatgatggc cctgttcacg gtcgtcggag ccgttgtgtc cctgttctgg ggctacttcg
    10141 cggacaaggc cagccggaag aagctgttcg tgttctctgt ggccctgggg gagataccct
    10201 gcgccttgac agctctgtcc ccatcttggg aagtcttctt cgccctgcgg atactgacgg
    10261 gaatcggggt gggggccgcg ttccctctgg ttttctccat tctgggggac atctataacg
    10321 agaaggagag gcccatggct tcggctatcc tgaccacctc cttcggcctc ggggccatag
    10381 ccgggccggt cctgggcggc tttgtaggcc aggccgcggg ctggcgtctg cccttcttcc
    10441 tggctgcggt tccgaatatt cccctgctct tagctttctg gttcctggtg cctgaacctc
    10501 gggccgcggc cagcgaggaa gccacccggg agctcgtgga agccggaatc ctctacccca
    10561 agcatatccg ggtccaggat tatgcccgcc taggcaaggt gcctacgaac gtctacctct
    10621 tcatccaggg catcgcgggg accatcccct ggggggcctt cttcttcctc aacaagtttt
    10681 tggaaacctc gaaggggtta tccgtggggc aggcaaccgc agtttacatg gtcttcgggg
    10741 cagggatggt cgcaggaaac cttgcgggcg gaaagctcgg agagctcgtg ttccggcgtt
    10801 ctgcaaaaga cctgcctctc ttctgcggag taacgacctt cctgggctgt ctgggcaccc
    10861 tgggagtggt cacgctccgg ggttccatcc ccctcctgtc cgccgcgggc ttcctggccg
    10921 ccttcttcgc cgcaatgacg ggccccaata tgcggaccat gcttctggac gtgaatcccc
    10981 ccgaagaccg gggacctatc ttctctatct tcaacctgac cgattccctg gggacaggcc
    11041 tgggtcgatg ggtggcgggg atgctttccg tggcggtggg gctttccggt gccctgacga
    11101 tcagcgcctc cttctgggtg atctgtggga tcgttctcgt ctttacagcg cggatcttcc
    11161 ccaaggatat cgggatccta aatgagcgta tggtacaaac cgcccgggag atgcggggac
    11221 agagctcaac gtaaacgatt gcctcgggga cagagctcga tacaagagac ccttccgggg
    11281 acagagctca tacggaaacg cggcgaaacg caagtgaact cgcaaactag tagcgtattg
    11341 tgggcatgag aaagccaagg gagctgcaag caggggcggt ataccatgtg acggccaggg
    11401 cgaaccgagg agagatgatt ctagacgcta aaggtctgaa ggacctcttt ctgcgtgtcc
    11461 ttgctcgggc tagaactagg taccggtttc gaatcgaaaa tttctgcgtg atggggaacc
    11521 atatccacct catgatccgg cccggggaac gggagtcctt gtcccggatc atgcagtgga
    11581 tcctcagtgt gttcgcgatg gcgttcaacc ggatacacgg gtacacggga catgtctggg
    11641 gatgtcgctt tttctcgagg atcatggcca gctttcaaca ctttgcgaga gcattcgagt
    11701 atatagacaa caatccggtg gaagccctcc gggtggaaac agcggagagt tggcttcatg
    11761 gagggttgtg gcatcaccgt catgggatac gggggatcgt ggatgagctc ggggagtatg
    11821 ccgcactggc tttcccaggg cataggcttc tactcttggg cgatgactag ccgcacctcc
    11881 gaccgggctc atcccgttag gagggcttcc gatgtcactt ctgttccagg tagtcacgga
    11941 gcagatcgtc gatgtcgtcc tgggcgatat cctccctgcg ctcgaatgcc agttcgaggt
    12001 tcgcctcccg gagtttcgcc agcaggctgt agatgatcat gaacaacacc ttgatgccag
    12061 ccgcagggtg ctcccgaatg aagcgcatga agtcggaccg tttgacgcgc aggaccgtca
    12121 ttccatcgtc cgccgccgcg acgttagcgg tacgcttcag gttcatgaac aagcccgcct
    12181 ccccgaagac atccccggcg ccgagggtac agatatacgc gtcgcgtccg ttctcttcga
    12241 cggtcacccg cgcgctcccg gacaggatga ggtacaggca gtcg
//
LOCUS       DAOTEI010000101        12273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3621_length_12273_cov_5.290391, whole genome shotgun sequence.
ACCESSION   DAOTEI010000101 DAOTEI010000000
VERSION     DAOTEI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12273
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3621_length_12273_cov_5.290391"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="P5313_10475"
     CDS             <1..688
                     /locus_tag="P5313_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY80829.1"
                     /translation="TSFCDVYYYNGFKQDIAAITEMVHAQGSLAYLDAYQGIGTHPID
                     VKALDVDILSSGNLKYLLGIPGIAFVYVKKELIPYMRPAFTGWFGQENPFAFDIHHLD
                     YASDARRFDNGTPPVLTSYIARAGMAIINEIGTEAIQAWTDRLSEHSLEGGRRRGFDI
                     ASPQDVRRKAPTTAFRVGSDSHAVEVALRERGIVASARGDVIRFAHHFFTKPEDLDYA
                     LDCLAEILKR"
     gene            708..1007
                     /locus_tag="P5313_10480"
     CDS             708..1007
                     /locus_tag="P5313_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY80830.1"
                     /translation="MLLAQVVGKADSSVKSPGISGSKLLVVRGLGPAGEPEGGLVIAV
                     DAVGAGPGDTVAVAQGGAALRAAGLEKIPCDAAVVAILDHVFLNGKEIISNRRTS"
     gene            1007..1285
                     /locus_tag="P5313_10485"
     CDS             1007..1285
                     /locus_tag="P5313_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY80831.1"
                     /translation="MKALGMIETRGYVAAIEAVDTMVKSASVEVLGTKEVGGGLVSVF
                     VQGDVGAVKAAVDAAAASVKKLGELVSVHIIPKPSDETVALIPAVPKK"
     gene            1298..1918
                     /locus_tag="P5313_10490"
     CDS             1298..1918
                     /locus_tag="P5313_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY80832.1"
                     /translation="MPDSGFQLRSYVFIDSMQPQYAAFLGSELDGDVPLAGMAELWLE
                     LAPGSEVYNLLDAALKSTLAKPGLQMVEREFGLLELHSESVDSVKDAGQSILAACGLT
                     ASDRIRPSVVSSKIISKVNPYQAQQINKTRKGALLLPGKTLLVMEVEPAANIVLAANA
                     AEKASEIQLVHFNPFGRFGRLFLSGTESEVRAASEAAHAAVSANAG"
     gene            1920..3527
                     /locus_tag="P5313_10495"
     CDS             1920..3527
                     /locus_tag="P5313_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY80833.1"
                     /translation="MAVRKGPFLPDWIGGDHGRRPAGEPVSSPRGARFLPDWIPSGRA
                     EDAAAAPPAGYPPPARGFSPGWARAPARGPLDEEPPGGRGRPLVVKGGHCVVPYAGIV
                     TQDLLIEDGRIKAMGRDLDTADRDVIRAEGKYVLPGLVDPHVHLGIFAPLDEEMATET
                     SSALVNGVTTVGLYLGGKGSYVPALESLIDLIQTKSRADAFVHLVLFDRDQLAEVPLY
                     ADRYGVRSFKVYMAGIPGLIPSADDGFLLDAMETVAALGEGAVLNIHAENSRVLEWAD
                     ARARMEFPQGGLRAWEASHPAFAEREAVQRALLLAERSGARLYFVHVSAAETADLIRR
                     AKAERPGRFYAETTSPYLSTDFDSPQGPLALMSPPIRSAQDRAALWRALEDGTLDTIG
                     TDHTPLTAEQKTGGRSFWETMPGYPAVGTHLPLLLDGARKHHMSVERLVEKVSANPAR
                     VFGVYPRKGTILAGSDADLVIVDMDRMKRISVEGARSRSDFALHQGELGIGWPVAVVK
                     GGRVLYPEDPRLDLEKFSGAAYLPRRR"
     gene            complement(3602..4258)
                     /locus_tag="P5313_10500"
     CDS             complement(3602..4258)
                     /locus_tag="P5313_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRY80834.1"
                     /translation="MRTLDEYPALDLLESRGIPAAARELADSEEAAVAAAARIGYPVV
                     FKISAPSALHKTEIGGVAVGIRDEAEARAAYRGILERAAAAGIVRMSGLRGVLVQEMV
                     KGGVEFILGGYRDPGFGPVVMVGLGGIFTELLKEAAFRLAPVDEAEARRMVLETRARV
                     LAGGFRGAPPLDLGPLCSAIAELSRMMSESEEIAEFDVNPFVLTPSGGRALDALFKYR
                     "
     gene            complement(4255..5676)
                     /locus_tag="P5313_10505"
     CDS             complement(4255..5676)
                     /locus_tag="P5313_10505"
                     /inference="COORDINATES: protein motif:HMM:NF039222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRY80835.1"
                     /translation="MDSFFDSAGCAVIGASANPSKAGYQILANMVAAGYPGGLYPVNA
                     SGGEILGRPSRPSLDAIENEVNLVVLSAPAAATPDILAQMDRRMRKQGDIRAVVCAAA
                     GFAEVKTPEGTAWQKSLTEFCRSRGIRLLGPNCVGVIDVNRKVDTTFIAGITHVPGGI
                     SFVSQSGAVGAWLLMNWAASPAGGVGFNKFVTVGNMADVDIIESVSYAGADPATRVLG
                     VYVEGSAEARRLVEAAGEVARDKPVAILKVGRTDEGAQAASSHTGSLAGTDALYDGAF
                     RQHGITRVRSVEELSDVLRAFDSLPLPAGNRVFILTQAGGPGILCVDELASAGIFQPA
                     RVSDATKSALKDCLPPIASICHPEGHADITAAAGAREHVESLEILLRDPGVDAVLFIT
                     VATLFLDLAAMARGMAEVVERLQGEGVSKPVLPAVLSGDWTRESRGILEKAGIPTFES
                     PERAVRALAALRSYAEFRGRRPS"
     gene            complement(5678..6595)
                     /locus_tag="P5313_10510"
     CDS             complement(5678..6595)
                     /locus_tag="P5313_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY80836.1"
                     /translation="MSALDLLAEREDMISPGISACVGCNVELTLRTVMKVLGPNSILA
                     IPPGCMGGVGVVGWNKQSGAKVPVFFPLLDNTASMLAGIRLALDAKGRDAHVVAFAGD
                     GATVDAGFQALSGVAERGDRIIYICYDNEGYMNTGYQRSSSTSKGSWTSTTPVSKEGQ
                     GGKRQHGKDFPMIMAMHDVPYVATASPAYIPDLVRKVEEAMKVKEGLAYIHVYNPCLT
                     GWGIRAEESISVARLAVKTRFFPLYEARNRKFKITVPVPNPLPLEDYTSRIKKFSHLG
                     PERIAELKDLAEERYRFLEAVSAGGGGRA"
     gene            complement(6592..7791)
                     /locus_tag="P5313_10515"
     CDS             complement(6592..7791)
                     /locus_tag="P5313_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylglyoxylate dehydrogenase"
                     /protein_id="HRY80837.1"
                     /translation="MSTVAVMNGNYAAATAARLARPEVIAAYPITPQTPVVEYLTLFA
                     AEGSLSSRMTEVESEHSAMSILSGASLAGARCYTATSSQGLALMYEPYFRASTLRLPI
                     VMNIVNREMISPQSVWGGPQDSLTLRDAGWIQIYVEDNQEILDQTIQAFRIAEDPRVL
                     LPINVCYDGFYLSHMTERVEVPEQAEIDAFLPPYEAKHQILDPRKPMAIDPLTSGYLV
                     MEYRKKHMDSMAAALGVVEETDKAYAALTGRSWGGALDLYRMEDAEYAVVTLGSVTGA
                     CREMVDEKRAAGVRIGLVKVRCLRPFPKAAVAAALARVKGFGVIDRNVSFGWNTGIVY
                     QEVRSALYESGRTLPSVPFVGGLGGEDITLGLVSKAFDEIVAAVDAGRTPQSAVWLWA
                     PRKGVKA"
     gene            complement(7788..8054)
                     /locus_tag="P5313_10520"
     CDS             complement(7788..8054)
                     /locus_tag="P5313_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY80838.1"
                     /translation="MSTLEKDYVCPVGEGIYAANTGDWRTQRPEIDQEKCSRCGTCLL
                     YCPTNSVRRVEGRFVIDLGYCKGCAVCAAECPKQCIAMIREGRK"
     gene            complement(8067..8615)
                     /locus_tag="P5313_10525"
     CDS             complement(8067..8615)
                     /locus_tag="P5313_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRY80839.1"
                     /translation="MKEIRLHGRGGMGTVKAAEIIVTAKVLEGGYGNSIPFFGFERQG
                     APVSSFVRLDERPIRPKTQVYTPDCVIVLDGTLRKAVDVFEGLKDGGWLILNSEPGSR
                     VEDLDLPRSVGTVVLVDATGLALRRLGKPITNTAMLGAFVKGTGWVELSDVASKVEEF
                     WGRENADLLGEAFEAAQVIRLR"
     gene            8854..9342
                     /locus_tag="P5313_10530"
     CDS             8854..9342
                     /locus_tag="P5313_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRY80840.1"
                     /translation="MNESLIPKKGPGADTVDDIDRKILAVLIQDSRITYSELAGIVGI
                     SRISVKERVLAMQKREIIEKFTIQIPAKFLGKPLPVFFEIQVSPETIHKVAEALSEHP
                     DITIVYQMTGMNCLHVHAFFVNVEDVSAFINEFLSSLPGIQSVNTQFLLKRYKADRSM
                     VV"
     assembly_gap    9403..9412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9413..10252)
                     /locus_tag="P5313_10535"
     CDS             complement(<9413..10252)
                     /locus_tag="P5313_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRY80841.1"
                     /translation="MTGHHEAWKVGAFTSRPFSGNPAGVVPDAAGLSDAYMQSAAREL
                     NDVSETVFILGGGGEWDYRFRYFTSSCEVDLCGHATIAGLFVLAWSGRIRGSEGTVPL
                     RIATRAGILNTGLAFRRGMPVRAFLEQPEPRTAPAPRADLAAGILGLEPELLDLDLPV
                     GCAFSGLWACFVPLRSVECLGRIRADRNRIQELWPDNPDLTGIYPFAFEDARTTRGRF
                     FVPPKFGIEEDPVTGTACGALGAFLFAKGRWPSAGYLQARQGVEMGRGGEVRVERTPG
                     GRMI"
     gene            complement(10249..11292)
                     /locus_tag="P5313_10540"
     CDS             complement(10249..11292)
                     /locus_tag="P5313_10540"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRY80842.1"
                     /translation="MNRIIDLRSDTFSLPDEGMRRAIRDAEVGNSGFREDPSVRKLEE
                     ACAAFFGLEAAVFLPSGTMAGQIALNVRSRPGDLVAIEENGHNLRFEAGSMALVSGTQ
                     ALTVRGDRGIMDPGRVRTAVTSARDHGARVSCIVLENTSNFGGGTIYPREVLEGMFRL
                     SGELGIPVHVDGARIWNALAATGEEPGSLIPQGGSVSACLSKGLGAPMGSILAGSDGF
                     VEEARRVQQLLGGVMRQVGFMAAAGLYALRNNLGRLREDHARCRRIAERLTDCAELEI
                     DLAAVQTNILYLRIRAGEAAAEDLRIRMAREGILAFRVGDRIRLVTYLNLDDGDCERA
                     ADTIASLLRERRA"
     gene            complement(11289..>12273)
                     /locus_tag="P5313_10545"
     CDS             complement(11289..>12273)
                     /locus_tag="P5313_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY80843.1"
                     /translation="SGKSFQGLPSLREKILARGGFRSGPGDVLVTAGTAEANFLAISQ
                     LVRPGDRMVVDVPGWPQPLVLGEAIGADVVRIRRREAEGWRLDLDELESLVTDRTRLI
                     FLCNPNNPTGRVLSRPELERAAGIADRVGAYLLCDEVYSGLEWDGQSTPRAADIYERG
                     ISTGSVSKVLGLQGLRTGWLVCRDPSLVLDAMVLREDTSEIMNVLGEYVADLALNEDR
                     YRTAVERARSIGSANLDRIDEFVASRRELSWERPQAGLIGFCRLEPPLDGDELSRILL
                     REPYRTFVMSGSAYGYPDHVRVGAGGSGPDGISRGLLKLGECLDALGEKRS"
BASE COUNT         2126 a         4199 c         3941 g         1997 t
ORIGIN      
        1 gacctccttc tgcgacgtct actactacaa cggcttcaag caggacatcg ccgcgatcac
       61 cgagatggtc catgcccagg gctccctggc ctacctggac gcctaccagg gcatcggcac
      121 ccacccgatc gacgtgaagg ccctggacgt ggacatcctg agctccggaa acctgaaata
      181 ccttttgggc atccccggta tcgccttcgt ctacgtgaag aaggagctga tcccctacat
      241 gcgcccggcc ttcacgggct ggttcggcca ggaaaacccc ttcgccttcg acatccacca
      301 cctggactac gccagcgacg cccggcgctt cgacaacggg acccccccgg tcctgacctc
      361 ctacatagcc cgggcgggta tggcgatcat caacgagatc gggaccgagg ccatccaggc
      421 ctggacggac aggctttccg aacacagcct ggagggcggc cgccgcaggg gcttcgatat
      481 cgccagcccc caggacgtgc gacgcaaggc gccgaccacg gccttccggg tgggttccga
      541 ctcccacgcc gtggaggtgg ccctccggga acgcgggatc gtggcctccg cccgggggga
      601 cgtcatccgc ttcgcccatc atttcttcac gaaacccgag gatctggatt acgctctgga
      661 ctgtctggcg gagatcctga agcgctgaag gcgcgggagg taaagctatg cttctggccc
      721 aggtcgtcgg aaaggccgac tcgtcggtga aatccccggg catctcgggg tccaagctcc
      781 tcgtggtgcg ggggctagga ccggccgggg agccggaggg gggactggtc atcgcggtcg
      841 acgcggtggg cgcgggcccc ggggacacgg tggccgtggc ccagggggga gcggccctca
      901 gggcggcggg actggaaaag atcccctgcg acgccgcggt ggtggccatt ttggatcatg
      961 tgttcttgaa cggcaaggag ataatttcga acaggaggac gagttgatga aagcgttggg
     1021 aatgatcgag acccgcggct acgtggccgc gatcgaggcc gtggatacga tggtcaagtc
     1081 cgccagcgtc gaggtcctag ggaccaagga agtgggcggc ggactcgtct ccgtgttcgt
     1141 ccagggcgac gtcggggccg tgaaggccgc tgtggacgcc gcagccgctt ccgtcaagaa
     1201 gctcggggaa ttggtgtccg tgcacatcat ccccaagccc agcgacgaga cggtcgcact
     1261 gatccccgcg gtcccgaaga aataaggaga gatcaggatg ccggattcgg gattccagct
     1321 gcgctcctac gtcttcatcg acagcatgca gccccagtac gccgccttct tgggctccga
     1381 gctggacggc gacgtgcctc tggcggggat ggcggagctg tggctggagc tggccccggg
     1441 cagcgaggtc tacaacctcc tggacgcggc cctgaaatcg accctggcca agccggggct
     1501 ccagatggtg gaacgggagt tcggcctcct ggaactccac tccgagtccg tggactcggt
     1561 caaggacgcg ggccagtcga tcctggcggc ctgcggactg accgcgtcgg accggatacg
     1621 gccttcggtg gtttcctcaa aaatcatcag caaggtgaac ccctaccagg cccagcagat
     1681 caacaagacc cggaagggcg ccctgctcct gcccggcaag accctgctgg tgatggaagt
     1741 ggaacccgcg gccaacatag tcctggcggc caacgccgcc gagaaggcct cggagatcca
     1801 gctggttcat ttcaatccgt tcggacgttt cggccggctc ttcctctcgg gcacggagtc
     1861 cgaggtgcgg gccgcgtcgg aagcggccca cgccgcggtc tcggcgaacg cgggctgaca
     1921 tggccgtaag gaagggcccc ttcctgcccg actggatagg cggggaccac ggccggaggc
     1981 ccgccggcga acccgtctca agcccccggg gagcccgctt ccttcccgac tggataccct
     2041 ccggccgggc ggaggacgcg gccgctgccc cgccggcggg ctacccgccc ccggcgcggg
     2101 gcttctcacc gggctgggcc cgggcgcccg cccgagggcc cctggacgaa gaaccccccg
     2161 gcgggcgtgg acggcccctg gtcgtcaagg ggggacactg cgtcgtgccc tacgcgggca
     2221 tcgtcaccca ggacctcctc atcgaggacg gacgcatcaa ggccatgggc cgggacctgg
     2281 ataccgcgga ccgggatgtg atccgggcgg aaggcaagta cgtcctgccc gggcttgtgg
     2341 atccccatgt ccacctgggc atcttcgccc ctctggacga ggagatggcc acggaaacct
     2401 cgtcggccct ggtcaacggg gtgaccaccg tggggctcta cctggggggc aagggctcct
     2461 acgtgcccgc cctggaaagc ctcatcgatc tgatccagac gaaatcccgg gcggacgcct
     2521 tcgtccacct cgtgctgttc gaccgggacc agctggcgga agtgcccctg tacgcggacc
     2581 gctacggggt acgctccttc aaggtctaca tggccggcat ccccgggctc atacccagcg
     2641 cggacgatgg tttcctccta gacgcaatgg agaccgtggc cgccctgggc gagggggcgg
     2701 tgctcaacat ccacgcggag aactcccggg tccttgaatg ggcggacgcc cgggcccgga
     2761 tggaattccc ccagggcggc ctgcgcgcct gggaggccag ccacccggcc ttcgcggagc
     2821 gggaggcggt ccagcgagct cttctgctcg cggagcgcag cggggcccga ttatattttg
     2881 tccacgtttc agccgcggaa accgcggacc tcatccgccg ggccaaggcg gagcgtcccg
     2941 gccgcttcta cgcggagacc acatcaccct acctctccac ggacttcgac agcccccaag
     3001 gacccctggc cctgatgagc cctcctatcc ggagcgccca ggaccgggcc gctctctgga
     3061 gggccctgga ggacggcacc ctcgacacga tcggaacgga ccacactccc ctgaccgcgg
     3121 aacagaagac cggcggccgg agtttctggg agaccatgcc cgggtacccc gccgtaggaa
     3181 cccacctgcc cctgctccta gacggcgccc ggaaacacca catgagcgtg gagcgcctgg
     3241 tagagaaagt ttccgccaat cccgcccggg tcttcggagt ctacccccgc aaggggacga
     3301 tcctggcggg ctccgacgcg gatctggtca tcgtggacat ggaccggatg aagaggatca
     3361 gcgtggaggg cgcccggtcc cggtcggact tcgccctgca ccagggcgag ttggggatcg
     3421 gctggcccgt ggcggtggtc aagggcggac gagtcctgta tcccgaggat ccccggctgg
     3481 acctggagaa gttttccggt gccgcctacc tgccccgccg ccgctgagag ttcagtcacg
     3541 ggtccggcaa aacagacggc cgccggtcat cccggcggcc gcgtcttttt tcaagccatg
     3601 atcagcggta cttgaacaat gcgtccaggg cccggccccc cgagggagtc agcacgaagg
     3661 ggttcacgtc gaactccgcg atttcctcgg actccgacat catccgggag agttccgcga
     3721 tcgcggagca gaggggaccc aggtcgaggg gtggagctcc ccggaatccc cctgccagca
     3781 cccgggcccg ggtttccagg accatgcggc gggcctccgc ctcgtcgacg ggagccagcc
     3841 ggaaggcggc ctccttgagg agttccgtga agatcccgcc caggccgacc atcaccaccg
     3901 gaccgaaacc ggggtcccgg tatcctccca ggatgaattc caccccgccc ttgaccatct
     3961 cctggaccag aactccccgg agtccggaca tccgcacgat accggcggcg gcggcccgct
     4021 ccaggatgcc ccggtacgcg gcccgggcct ccgcctcgtc ccggatcccg acggcgacgc
     4081 cgccgatctc ggtcttgtgc agggccgaag gcgcggaaat cttgaacacg acggggtagc
     4141 cgatccgggc ggccgcggcg actgcggcct cctcgctatc cgccagttcc cgggcggcgg
     4201 cggggatgcc ccggctctcc aggaggtcca gggcggggta ttcgtccagg gtcctcatga
     4261 aggcctcctt ccgcggaact cggcatagct tcgaagggcg gccaaggcgc gcacggccct
     4321 ctcggggctc tcgaaggtgg ggatgcccgc cttctccagg atcccccggg actcccgggt
     4381 ccagtccccg gaaaggaccg cgggcaggac cggcttggaa acgccctcgc cctggaggcg
     4441 ttccacgacc tccgccatgc cacgggccat ggcggccagg tccaggaaga gggtcgccac
     4501 ggtgatgaag aggactgcgt ccaccccggg atcccgcagg aggatctcca gggattccac
     4561 gtgctcccgg gcccccgcgg cggcggtgat gtccgcgtga ccctcggggt ggcagatcga
     4621 ggcgatgggg ggcaggcagt ccttgagggc ggacttggta gcgtccgaga cccgggcggg
     4681 ttggaagatg ccggccgatg ccagctcgtc cacgcagagg atacccgggc cgccggcctg
     4741 ggtcaggatg aagacccggt ttccagccgg caggggcagg gagtcgaagg cccggagtac
     4801 gtccgagagc tcctccaccg agcgcaccct ggtgatcccg tgctggcgga aggccccgtc
     4861 gtagagtgca tcggtcccag ccagggatcc cgtgtgggag ctggccgcct gggcgccctc
     4921 gtcggtgcgg ccgaccttga ggatcgccac gggcttatcc cgggccacct cgcccgcggc
     4981 ctccaccagg cgccgtgcct ccgcggatcc ctccacgtac accccgagca cccgggtcgc
     5041 gggatcggcg cccgcgtagg atacggactc gatgatgtcc acgtccgcca tgttccccac
     5101 ggtcacgaac ttgttgaaac ccactccccc ggcgggggag gcggcccagt tcatgaggag
     5161 ccaggcgcct acggccccgc tctgggacac gaaggaaatc cctcccggca cgtgggtgat
     5221 cccggcgatg aaggtggtgt ccaccttccg gttgacgtcg atgaccccca cgcaattggg
     5281 ccccaaaagg cggatccccc gggaacggca gaactcggtc agggacttct gccaggcggt
     5341 cccctcggga gtcttcacct ccgcgaagcc cgcggccgcg cagaccacgg cccggatgtc
     5401 gccctgcttc cgcatgcgcc ggtccatctg ggccaggatg tccggggtgg ccgcagccgg
     5461 cgcggagagc accacgaggt tcacctcgtt ctctatggca tccagggacg ggcggctcgg
     5521 gcggcccagg atctccccgc cggaggcgtt cacgggatag aggccccccg ggtatcccgc
     5581 ggcgaccatg ttggccagga tctggtatcc cgccttggaa ggattcgcgg acgcgccgat
     5641 cacggcgcag cccgcggagt cgaagaagga atccatgcta ggcacggccg ccgcccccgg
     5701 cgctgaccgc ctccaggaag cggtaccgtt cctcggccag gtccttgagc tccgcgatcc
     5761 gctccggccc caggtgggag aacttcttga tccgggaggt gtagtcctcg agcggaaggg
     5821 gattcggcac gggtacggtg atcttgaact ttcggttccg ggcctcgtag agtgggaaaa
     5881 accgggtctt gacggccagg cgggcgacgg agatcgactc ctccgcccgg atcccccagc
     5941 ccgtcaggca cgggttgtac acgtggatgt aggccaggcc ctccttgacc ttcatggcct
     6001 cctcgacctt ccgtaccagg tcggggatgt aggcgggaga cgccgtggcc acgtagggca
     6061 cgtcgtgcat ggccatgatc atggggaagt ccttgccgtg ctggcgcttc ccgccctgcc
     6121 cctccttgct gaccggggtc gtgctggtcc aggacccctt ggaggtcgag gaactgcgct
     6181 ggtacccggt gttcatgtag ccttcgttgt cgtagcagat gtagatgatc cggtcccccc
     6241 gctcggcgac cccggagagg gcctggaagc ccgcgtccac ggtggcgccg tcgccggcga
     6301 aggccaccac gtgggcgtcc cggcccttgg cgtccagggc cagccggatg ccggccagca
     6361 tgctggccgt gttgtccagg agggggaaga agacgggaac cttggccccg gactgcttgt
     6421 tccagccgac caccccgacg ccgcccatgc agcccggggg tatggccagg atcgagttgg
     6481 ggcccaggac cttcatcacg gtcctcaggg tgagttccac gttgcacccc acgcaggcgg
     6541 agatgccagg ggatatcatg tcctcgcgct cggcgaggag gtccagggcg ctcatgcttt
     6601 cactcctttc cggggcgccc agagccagac cgcgctttgg ggggtccggc ccgcgtccac
     6661 ggcggccacg atctcgtcga aggccttcga tacgagaccc agggtgatgt cctcgcctcc
     6721 gagcccgccg acgaagggga ccgaggggag ggtcctgccg gattcgtaca gggcggaacg
     6781 gacttcctgg tacacgattc cggtgttcca tccgaacgac acgttccggt cgatcacgcc
     6841 gaagcccttg acccgggcca gggccgcggc gaccgccgcc ttcgggaagg gccggaggca
     6901 gcgcaccttc acgaggccga tccggactcc cgcggcgcgc ttctcgtcca ccatctcccg
     6961 gcaggccccg gtgacggaac cgagggtgac caccgcgtac tcggcgtcct ccatgcggta
     7021 gaggtccagg gcgccgcccc aggaacggcc ggttaacgcc gcgtaggcct tgtccgtctc
     7081 ctcgacgacc cccagggccg cggccatgga gtccatgtgc ttcttgcggt actccatgac
     7141 caggtagccg ctggtgagcg gatcgatcgc catgggcttg cgggggtcca ggatctgatg
     7201 cttcgcctcg tagggaggta ggaaggcgtc gatctcagcc tgttccggaa cctccacgcg
     7261 ctcggtcatg tgggataggt agaaaccgtc gtagcagacg ttgatgggca ggaggacccg
     7321 gggatcctcg gcgatgcgga aggcctggat agtctggtcc aggatctcct ggttgtcctc
     7381 cacgtagatt tggatccatc ccgcgtcccg gagggtcagg gaatcctggg gtccgcccca
     7441 gaccgactgg ggggatatca tctcccggtt cacgatgttc atcacgatgg gaagtcggag
     7501 ggtggaagcc cggaaatagg gctcgtacat cagggccaat ccctgggagg aggtggcggt
     7561 gtagcagcgc gctccggcca gggacgcccc gctcaggatg gacatggcgg agtgctcgga
     7621 ctccacctcc gtcattcggc tggacaggga gccctccgcg gcgaagaggg tcaaatactc
     7681 cacgacagga gtctgggggg tgatggggta cgcggcgatc acctccggcc gagccaaccg
     7741 ggcggccgtg gcggcggcgt agttcccgtt catgacggct acggtgctca tttccttccc
     7801 tcccggatca tggctatgca ctgcttcggg cattcggcgg cacagaccgc gcagcccttg
     7861 cagtagccca ggtcgatcac gaaacggcct tcgaccctcc ggacggagtt ggtcggacag
     7921 tagaggaggc aggtgccaca gcggctgcac ttctcctggt ctatctccgg acgctgggtc
     7981 cgccaatccc cggtgttggc ggcgtagatg ccctcgccca ccggacatac gtagtctttt
     8041 tccaacgtgc tcatgatttc gctccctcaa cgcaaccgga tgacctgggc cgcctcgaac
     8101 gcttcgccga ggaggtccgc gttctccctg ccccagaact cctccacctt ggaggccacg
     8161 tccgaaagct caacccaccc cgtgcccttc acgaaggcgc ccagcatcgc cgtgttcgtg
     8221 atgggcttgc ccaacctgcg aagggcaagt cccgtggcgt ccaccaggac gactgtccca
     8281 acgctacgag gcaggtccag atcctccacc cgggatcccg gttcggagtt caagatgagc
     8341 cacccgccgt ctttgaggcc ctcgaacacg tcgacggcct tgcgaagggt cccgtccagg
     8401 actatgacgc agtccggggt atagacctgt gtcttgggac ggatgggtcg ttcgtccagg
     8461 cgcacgaagg agctgaccgg cgcgccttgc cgctcgaatc cgaaaaaggg gatggaattg
     8521 ccgtaaccgc cctcgagcac cttggcggtg acgattatct cggcggcctt gacggttccc
     8581 atccctccgc ggccgtgaag ccgtatttct ttcatctgga tactcctcct gatatcagga
     8641 acatcacacg atttcactta atgtatcacg attaccgatt atgatcaaca tatagtttga
     8701 ttggtgctat aatatctttc attctgaaag ccgatgtctc ccacggaaga cttttcgaca
     8761 attcaacgcc cgggagccgg acgattgcgt taccggcccg aggcacgtac aatgcccaag
     8821 aaccattccg gaaacatgga aggcggttgc gctatgaacg aaagcctcat tcccaaaaaa
     8881 ggccccggcg cggatacggt ggatgatatc gaccgaaaga tcctggcggt cctgatccag
     8941 gacagccgga tcacctattc cgaactggcg ggtatcgtcg gaatttcccg catcagcgtc
     9001 aaggagcgag tcctggccat gcagaagcgg gagatcatcg agaaattcac gatccagatc
     9061 cccgcgaaat tcctgggaaa acccctgccc gtcttcttcg agatccaagt ttccccggag
     9121 accatccaca aagtcgcgga agcactctcg gaacacccgg acatcaccat cgtctaccag
     9181 atgaccggga tgaactgcct ccacgtccac gcctttttcg tgaacgtgga ggacgtgtcc
     9241 gccttcatca acgagttcct gtcgagcctc cccggaatcc agagcgtcaa cacccagttc
     9301 ctgctcaagc ggtacaaggc cgaccggagc atggtggtct agcggatccc tacggcttaa
     9361 gccaggagtc tcccccggaa gatcgcgcac gcgccccccc ccnnnnnnnn nngatcatcc
     9421 ttccgcctgg ggtccgttcc actcgaacct cgccgccccg ccccatctcg acgccttggc
     9481 gagcctgaag gtatccggcc gaaggccacc ggcccttcgc gaagaggaac gcgcctagcg
     9541 ccccgcaggc ggttcccgtt acgggatcct cctcgatgcc gaatttaggc ggcacgaaaa
     9601 aacgcccccg ggtcgttcgg gcatcctcaa aggcgaaggg atagatgccg gtcaagtcag
     9661 gattgtcggg ccatagttcc tggatccgat ttctgtctgc ccggatccgc ccgagacact
     9721 ccacggatcg tagagggacg aaacatgccc ataagccgga gaaggcgcag cccaccggca
     9781 gatccagatc caggagttca ggttccaaac ccagtatacc cgcggcgagg tccgcgcgag
     9841 gggcaggtgc cgtccggggc tccggctgct ccaggaacgc ccgcaccggc atgccccgcc
     9901 ggaaggcaag gcccgtgttc aggatgcccg cacgggtcgc gatcctcaga ggaaccgttc
     9961 cctcggatcc ccggatgcgc ccggaccaag ccagaacgaa gagacccgcg atcgtcgcgt
    10021 ggccgcataa atccacctcg caggaggagg tgaaataccg gaaccggtaa tcccactctc
    10081 ctcctccccc caggatgaaa acggtctcgg atacgtcatt cagttcccgg gccgcgctct
    10141 gcatgtacgc gtcactaagc cccgccgcat ccggcacgac acccgcggga ttcccggaaa
    10201 acggccggga cgtgaaggcg cccaccttcc aggcttcatg gtgtcctgtc atgcgcgccg
    10261 ctcccggagc aggctcgcga tcgtgtccgc cgcccgttcg cagtccccgt cgtccaggtt
    10321 caggtacgtg accagacgga tccggtcccc cacccgaaag gccaggatac cctcccgggc
    10381 catccggatg cgcaagtctt ccgccgctgc ctccccggcc cggatgcgca ggtagaggat
    10441 gttcgtctga accgccgcta gatcaatttc aagctccgca cagtccgtca atcgttccgc
    10501 tatacgccgg catcgtgcat ggtcctcccg gaggcgtccg agattgttcc gaagggcata
    10561 cagtcccgcg gcggccatga atccaacctg ccgcatgacc ccgcccaaaa gctgttgcac
    10621 acgccgggcc tcctcgacga atccatccga tcccgcgagg atgctcccca tgggggctcc
    10681 cagccccttg gaaaggcagg cggatacgga tccaccctgc ggaatcaggc tcccgggttc
    10741 ctctcccgtg gcggccaggg cgttccatat ccgcgctccg tcgacgtgga cgggtatccc
    10801 gagctccccg gagagccgga acatcccctc caacacctcg cgggggtaga tcgttccacc
    10861 cccgaagttg gatgtgtttt ccaggacgat gcacgaaacc cgggccccgt ggtccctcgc
    10921 ggaggtcacg gctgtgcgga cccgccccgg atccatgatt ccccggtccc cccgcacggt
    10981 cagagcctgg gtgccggata ccagagccat ggatcctgcc tcgaacctca ggttgtggcc
    11041 gttttcctcg atggccacga ggtccccggg gcgggatctc acgttcaacg ctatttggcc
    11101 ggccatggta ccggagggca ggaagaccgc ggcctccagt ccgaagaatg ccgcgcacgc
    11161 ctcctcgagc ttccgcaccg acggatcctc ccggaacccc gagttcccta cctcggcgtc
    11221 ccggattgcc cgccgcatcc cttcgtcggg aagggagaac gtatccgatc gaaggtctat
    11281 gatcctgttc acgatcgttt ctcccccagg gcgtccaggc attccccgag cttgaggagc
    11341 ccccgggaga tgccgtccgg accggagccc ccggccccga ctcggacatg gtccggatac
    11401 ccgtacgcgg acccggacat gacgaaggtg cgatagggct cccgcaggag aatccgtgaa
    11461 agctcgtccc cgtcaagggg aggctcgagc cggcaaaacc cgatcaagcc cgcctgggga
    11521 cgctcccagg acaactcccg tctggacgcc acgaactcgt cgatccggtc cagattcgcg
    11581 ctcccgatcg accgggccct ctccaccgcc gtccggtacc gatcctcgtt cagggcaagg
    11641 tccgccacgt attcgcccaa aacgttcatg atctcgctcg tgtcctcccg aaggaccatg
    11701 gcgtccaaga ccagactcgg atccctgcag accaaccacc cggtccgcag cccctggaga
    11761 cccaagacct tggaaacgga tcccgtggag atccctcgct cgtagatgtc cgccgcccgg
    11821 ggggtggact gcccgtccca ttcgagtccg gaatagacct cgtcgcagag gagataggcc
    11881 cccactcggt ccgctatccc cgcggcccgc tcgagctccg ggcgggaaag gacccggccg
    11941 gtgggattgt tgggattgca gaggaagatg agccgcgttc gatccgtcac cagggattcg
    12001 agctcgtcca ggtcgagccg ccagccttcc gcctcccgtc tccgaatccg gacgacgtcc
    12061 gcgccgatcg cctctcccag gaccaggggc tggggccagc ccggaacgtc cacgaccatc
    12121 ctgtcccccg ggcggacgag ctgggagatc gccaggaagt tcgcttccgc ggtgcccgcg
    12181 gtcacgagga cgtccccggg accggaccgg aaccctcccc gggcgaggat tttttctcgg
    12241 agggagggca ggccttggaa ggacttaccc gac
//
LOCUS       DAOTEI010000102        12076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3693_length_12076_cov_4.636220, whole genome shotgun sequence.
ACCESSION   DAOTEI010000102 DAOTEI010000000
VERSION     DAOTEI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3693_length_12076_cov_4.636220"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /gene="mutL"
                     /locus_tag="P5313_10550"
     CDS             complement(<1..1654)
                     /gene="mutL"
                     /locus_tag="P5313_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRY80844.1"
                     /translation="MDSAPRIRILPPDTAKRIAAGEVIERPASVLRELLDNALDAGAS
                     VIEADLEGGGIASLRVTDDGRGMGPEDLALSILPHATSKIAELDDLLTARTLGFRGEA
                     LASVAAVARLSILSAERDGVPHLLTSVPGSPASVEPAAGRPGTTVRVRGLFDSYPARK
                     LFLKRPATESALCRQALVDKAIPFPSTAFRLFTEGALSLFLPPAGPAERVASVYAPDA
                     PAGLVSEVALSGPGFEGRTVIGDPALARTDRRHSQVFVNRRRVAEYSLLQALDWAFSP
                     VLPGGQHPFAFLFLDIDPALVDFNIHPAKREVRFRDIEPVRKAVVRAVREALSDRIRL
                     AGLRDREDVPERSLWEVRETPGLRDYPPRAPGTDPGALPGGTAEPEGRAGWDRVADLA
                     AGARRAREAESGAEARGGGSAPAGGPSPATDGGPGFRYLGSALGVFLVYEEDGALWLL
                     DQHAAHERLIFDRLVARPSTVQELLVPLVLEPDSDMEEVWLAASVETLKEAGYILERT
                     GSDWLVTGLPAGLASSDASAVLELLRSRPSPDALLREAHATTA"
     gene            complement(1911..4052)
                     /locus_tag="P5313_10555"
     CDS             complement(1911..4052)
                     /locus_tag="P5313_10555"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRY80845.1"
                     /translation="MKSRKLLAILAALLAPALLAAQGGPSMILEFVSGSELTVQIGGT
                     TLAYPSGIAEDDAIPVGALVKTGPTTTAELRLKPNGTLVKLAKNTNFRVDAVAAAPSQ
                     TNQFALLGGKVRAVAAKGGSYEIRSTSTVAGVRGTDFTFAVEEGMKNLLLVSKGAVQF
                     GRLGTDGAVLQSILVGAGQFADLFGSAFQALAYTAEQFAAEYGDVDISPDKLKSVPGQ
                     DVADAGGDDQGEDGDKEKEEEPSEDSPFVKWLKEALGFELGSVTIDQTTYAKAVIQPI
                     IKVGKLKMGLYLPVIYQTNLFDPGDWYRPRGNDEWSFGTDIGWRSDPAEAALDAVQDL
                     ALKIRFLEYGRQLEDPFYLKVGNLNTMTLGHGLLMRNYANDQDFPAVRRIGLNMGVTG
                     EKTGFEVVTNDLADPSVVGARLFFFPVKDFPFSLGAQAVADFFPARELAAPSSVGDPI
                     FLGLGLDMDLPIIRRNALLGIRWFVEGAATVPYIRDSFGTVDPGLKTELLWSDGQLKN
                     WGAATGFIGNVAIIDWRLEFRYFTGAFRPAFFDSAYERVKGAYAEEYAAYLTTPATYD
                     ATPNVMGVYGEGGFSFLREKLTFKMGYFWPWSPDAASLADIAGDDYFTAQLVIKKGLI
                     PVVDVYGSISYEKRGLAQDLFYDNDFALLDANTTLKGEVVVPVPKAPNLDLAFVFSTS
                     ARRDGNGDILYASPGIPEMVPVITLETRLHF"
     gene            complement(4063..5406)
                     /locus_tag="P5313_10560"
     CDS             complement(4063..5406)
                     /locus_tag="P5313_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80846.1"
                     /translation="MRKSIAVATILLLLSISAFAQDSEDSGSGFQFQAGINLGTDVLP
                     DGLGGFSSWTRLGFQPDLSFGKIGIGLDITLRFKLSPAPGEDAVYGGDWIPDYEGNGK
                     TFLDVYLPKFMYVRYGQRGDPLYVKLGSIQDLTLGDGFIVGDYANTRFLPEQRIFGLA
                     LDIDGALFQFPWVGAQLMTGNLARFDVVGARLFARPLYGTGIPIMESMQVGLTAAADF
                     QADLYNPVVQPLETVAMYGLDVFIPILGGKTFPLAAFSDFVLQPEGRWGSMIGAGGRL
                     FRFITYGAQLRVLGPGFIPVYFDANYDLYRAAKHDLMAVAPAGDTFTGWFASLGFSVL
                     EDKIAFKTSLDGPFAAKPAVATDNPADYPHLRAVATLGEGIVGGFSFDAIYEKYFLGK
                     TGTFFEDLVTAEDAVITAQINYRTGAAVLSLLYNLKYDGAGGYTVTSSLQSSIKF"
     gene            5610..7190
                     /gene="pyrF"
                     /locus_tag="P5313_10565"
     CDS             5610..7190
                     /gene="pyrF"
                     /locus_tag="P5313_10565"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRY80847.1"
                     /translation="MDFFSRLSAAWIERRTLLCVGLDPRPAAGAEDPRKAAAEILDRN
                     RRLIDATAEHAACFKPNIAFYEAWGSAGWDALKATIDYIPESVPVLLDAKRGDIDSTA
                     RAYARAVFRELGVDAVTLSPYLGRDAADPFLEYPDAALFLLCRTSNPRAGAFQDLTVD
                     GAASRTAVISGTGRAVGTARTSAEPLYLRVARECVSWSDRVGLVVAGNDPEALRAVRA
                     AAPEAWFLAPGIGAQGGDISEAWEAGAREDGLGMIPAVSRAVSEAPDPGAAAAALRRA
                     AAEAWEKAMESRIHEQKRPAPVSGVASPTAVPGRGTGLGNPSESETGTELDRGIIKEK
                     LLRGLVETGCFRVGEFTLKSGIRSPFYIDLRSVVSDAGLLETAAEAYALLARGLEYDR
                     LGGIPAAALPLATAAALRLRKPLVWPRMPVKEHGTGNRVEGRFRIGEKVLLLDDLITT
                     GASKLEAAEILRGEGLEVADLAVLIERGVRGRGELEAVGIRLHAFLHVRELIALCVSL
                     GLVDADAGRRMEEFAAAE"
     gene            complement(7221..7631)
                     /locus_tag="P5313_10570"
     CDS             complement(7221..7631)
                     /locus_tag="P5313_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY80848.1"
                     /translation="MRITTKGRYALRAVLALARTSEDGSPVSIKAISEREQISPEFLE
                     QIFFRLRKAGVINSVRGPGGGFYFARPLREITLEAILESSGEGLGIAPCVCGKEDACE
                     RRNDCAAGKIWREMDSHLRSFTAHRTLEDMVRGA"
     gene            complement(7736..8695)
                     /locus_tag="P5313_10575"
     CDS             complement(7736..8695)
                     /locus_tag="P5313_10575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY80849.1"
                     /translation="MPDSPDRQEEYLENRLRKRDRHLRKWARRESVECYRVYDRDIPE
                     IPLAVDRYGPYALVSLYERPYEKPEAEERAWLDRMCRAVSRVLEVPEEHIFRKVRRRQ
                     RGSGQYERVSEEGRTAVVQEGGLRFLVNLSDYLDTGLFLDHRITRSRVRDEARDRRVL
                     NLFCYTGSFSVYAAAGGAASVDSVDLSKTYLAWAGRNLDLNGFRGRSYSLFHEDALEF
                     LSSARAAGRRWDLAILDPPTFSNSKRMSGSLDINRDWIDLVSLCLEVLSPGGILYFST
                     NSRSFRFDETRFPGISLRDLTRPTTPPDFEDARPHRCWRLERS"
     gene            complement(8699..9343)
                     /locus_tag="P5313_10580"
     CDS             complement(8699..9343)
                     /locus_tag="P5313_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRY80850.1"
                     /translation="MARLKISFTPSIRRLPSYLHIIRNAQRNSQFYISGTVIAQELNL
                     EPIQVRKDLAITGIIGKPKKGYPVDALIAAVERYLGWDEMHDAIVVGAGNLGSALMGY
                     PEFRIHGLHIVAAFDTDPDKIGKKIHGVTVLNLADLEATIRGSKVEYAILTVPSPHAQ
                     ETADTLVKSGIRAIWNFTNAKLKVSDEVVVHREDLSSGYALLSVMLQTRKNQPA"
     gene            9717..11876
                     /locus_tag="P5313_10585"
     CDS             9717..11876
                     /locus_tag="P5313_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY80851.1"
                     /translation="MKRASRILVFLIVLVLPFAAAASEESGIAARETALVIQIGIILF
                     AVRLGSALAQRVGVPTVLGELLAGIVIGPYALGALPLPGFPTGLFAGSMGELAVSTEL
                     YGIATIASILLLFVSGLETDLSLLMKYSLAGGIIGIGGVALSFAAGDLLGMVLFRTGA
                     MDPRALFLGIMSTATSVGITARILSDRRKMDSPEGVTILAAAVFDDVLGIVFLAAVMG
                     IATLTRDGTGGPLPWGSILGIAGRAFGFWLGFTALGLLLSKQLARFLKSFRNPAYFSI
                     LALGLALILAGIFEKQGLAMIIGAYVLGISLSKSDISHVILDKTRGLYEFLVPLFFAV
                     MGMLVNVRQLLDPAVLLGGLAYSLVSVASKFIGCGFPALFLGFNGKGALRIGAGMVPR
                     GEVALIVAGIGLSGGLLSENEFGMAILMTLITTIAAPPVLSAALRIPGRGTRAVEKGA
                     EAESSVYAFPSEDVAVLVSDVLVRDLQNEGFFVQLMNVGDGIVQVRKDDIAFSLERKG
                     VELTLRMDPSDAIFVKTALFEAVAQLNQRFEELRKNLDPKRLTEGLDEEGGRVNPGFR
                     EALSPDCVILNLKGADKESVITELVDRLSSKGLLADRAQVLRDVLDREKSMSTGMQHG
                     IALPHAKTEGVTRIVAALGLKPEGVEFQSLDGAPAKVILLLASPGRQAGPHIQVLASA
                     AALLNDEIRRTDLLAAQSVEEAAVILGLGTKKAVSRD"
BASE COUNT         1997 a         3852 c         4156 g         2071 t
ORIGIN      
        1 aagccgtggt cgcgtgggcc tcccggagga gggcgtccgg ggacggccgg gaccgcagga
       61 gctccaggac cgccgacgcg tccgagcttg ccaggcccgc gggcaggccc gtgaccagcc
      121 aatccgaccc cgttcgctcc aggatatagc ccgcttcctt cagcgtctcc acggaggcgg
      181 ccagccaaac ctcttccatg tccgaatccg gctccaggac caggggcacg aggagctcct
      241 gcaccgtcga gggccgggcc acgaggcggt cgaagatcaa ccgctcgtgg gcggcgtgct
      301 ggtctaggag ccagagggcc ccgtcctcct cgtagaccag gaagaccccc agggcggagc
      361 cgaggtaacg gaagcccggc cccccgtccg ttgcgggaga gggacctccc gccggggcgg
      421 aacccccgcc gcgcgcctcc gcgccggact cggcctcccg ggcccgccgg gcccccgcgg
      481 ccaggtccgc gacccggtcc cagcctgccc gtccctccgg ctctgccgtg cccccgggga
      541 gggcgcccgg atcggtgccc ggagcccgcg gggggtagtc ccgcaggccc ggggtctccc
      601 ggacttccca gaggcttcgc tccggtacgt cctcccgatc ccggagcccg gccaggcgga
      661 tacggtcgga cagggcctcc cggaccgcgc ggaccacggc cttccgcacg ggctcgatgt
      721 cccggaaccg gacctcccgt ttggcggggt ggatgttgaa gtccaccagg gccggatcga
      781 tgtccaggaa gaggaaggcg aaggggtgct gcccccccgg caggaccggg gagaaggccc
      841 agtccagggc ctgaagcagg gagtattccg ccacccggcg gcggttcacg aagacctggg
      901 agtgccggcg gtccgtccgg gccaaggcag gatccccgat caccgtccga ccctcgaagc
      961 ccggacccga taaagcgacc tccgacacga gccccgccgg ggcgtcgggg gcgtagaccg
     1021 aggcgacccg ctccgccggc cccgccgggg gtaggaagag ggacagagct ccctcggtga
     1081 agaggcggaa ggccgtggag gggaagggga tggccttgtc caccagggcc tggcggcaga
     1141 gggcggactc cgtggccggg cgcttgagga atagctttcg ggcggggtag gagtcgaaga
     1201 gcccccggac ccggaccgtg gttccgggcc tgcccgccgc gggttccacg gaagcgggac
     1261 tgcccgggac ggaggtcaag agatggggga ccccgtccct ctcggcgctc aggatcgaaa
     1321 gccgggctac cgcggcgacc gaggccaggg cttcccctcg gaaacccagg gtgcgggcgg
     1381 ttaaaaggtc atccagttcc gcgatcttgc tggtggcgtg gggcaggatc gagagggcca
     1441 ggtcctcggg acccatcccg cggccgtcgt cggtgacccg gagggacgcg atgcccccgc
     1501 cctccaggtc cgcctcgatg accgaggccc ccgcgtccag ggcgttgtcc aggagttccc
     1561 ggagcaccga ggcgggccgc tcgatcacct cccccgcggc tatgcgcttg gccgtgtccg
     1621 gagggaggat gcggatgcgg ggtgcgctgt ccatgggaag gttataccgt ttccggaacc
     1681 gcggccgcaa tgcccggcgt ttaccggata gatccgacag gatggacagg atgaagaaag
     1741 atgaacaaga ttagtttttt agccatactc tatttctcgg tacgaaatta tcatgtttat
     1801 cttgtcttat cttgttaatc ctgttggaat tattaaaaaa aggccgcccg gaggcggcct
     1861 atgtaaaact tgaagggaga ggggcggccg gggcggccgc ccggattcgg tcagaagtgc
     1921 agccgcgtct ccagggtgat caccgggacc atctcgggga tgccggggga ggcgtacagg
     1981 atgtcgccgt tcccgtcccg ccgggccgag gtgctgaata cgaaggccag gtccaggttc
     2041 ggggccttgg gcaccggcac gacgacctcg cccttgaggg tggtgttggc gtccaggagc
     2101 gcgaagtcgt tgtcgtagaa gagatcctgc gccagacccc gcttctcgta ggagatcgag
     2161 ccgtatacgt ccaccacggg gatcaacccc ttcttgatga ccagctgggc cgtgaagtag
     2221 tcgtcccccg cgatgtccgc caggctcgcg gcgtccgggg accagggcca gaagtagccc
     2281 atcttgaagg tgagcttctc ccgcaggaag ctgaagccgc cctcgccgta cacgcccatc
     2341 acgttggggg tcgcgtcgta ggtcgcgggc gtggtcaggt aggccgcgta ctcctcggcg
     2401 taggcgccct tgacgcgctc gtaggcggag tcgaagaagg cgggccggaa ggccccggtg
     2461 aagtaccgga actccaggcg ccagtcgatg atggccacgt tcccgatgaa gcccgtggcg
     2521 gcgccccagt tcttgagctg gccgtcggac cagagtagct cggtcttgag gcccggatcc
     2581 acggtgccga agctgtcccg gatgtagggc accgtggcgg cgccctcgac gaaccagcgg
     2641 atccccagga gggcgttgcg ccggatgatg ggcaggtcca tgtccaggcc caggcccagg
     2701 aagatcgggt cgccgacgct ggaaggcgcc gcgagctccc gggcggggaa gaagtccgcc
     2761 accgcctggg cgcccaggga gaaggggaag tccttgacgg ggaagaagaa gagccgggcg
     2821 cccaccacgg aggggtccgc caggtcgttc gtaaccacct cgaagccggt cttctcgccg
     2881 gtgacgccca tgttgaggcc gatccggcgc accgccggga agtcctggtc gttcgcgtag
     2941 ttccgcatga ggaggccgtg ccccaacgtc atggtgttga ggttgcccac cttgaggtag
     3001 aagggatcct ccagctgtcg gccgtactcc aggaagcgga tcttgagggc caggtcctgg
     3061 acggcgtcca gggctgcctc tgcggggtcg ctcctccacc cgatgtcggt gccgaaggac
     3121 cactcgtcgt tgccgcgggg ccggtaccag tcgccggggt cgaagaggtt ggtctggtag
     3181 atgacgggca ggtagagacc catcttcagc ttgccgacct tgatgatggg ctggatgacc
     3241 gccttggcgt aggtggtctg gtcgatggtc accgatccga gctcgaagcc cagagcctcc
     3301 ttgagccact tcacgaaggg gctgtcttcg gagggttcct cctctttttc cttgtctccg
     3361 tcttcgccct ggtcgtcccc gccggcatcc gcgacgtcct ggccgggcac ggacttgagc
     3421 ttgtcggggc tgatgtccac gtccccgtac tccgcggcga actgctccgc cgtataggcc
     3481 agggcctgga acgcggagcc gaagaggtcc gcgaactgcc ccgccccgac caggatggac
     3541 tgcaagaccg ccccgtccgt gcccaggcgc ccgaactgga cggctccctt ggacacgaga
     3601 aggaggtttt tcatgccctc ctccacggcg aaggtgaagt ccgtgccccg gactcccgcc
     3661 accgtggagg tggagcggat ctcgtagctc ccgcccttgg cggccacggc ccggaccttg
     3721 ccgcccagga gtgcgaactg gttggtctgg ctgggcgccg ccgcgaccgc gtccacccgg
     3781 aagttcgtgt tcttggccag cttcacgagg gtgccgttgg gtttgaggcg gagctcagcg
     3841 gtagtggtgg ggccggtctt gaccagggcc ccgaccggga tggcgtcgtc ctcggctatg
     3901 cccgaggggt aggcgagggt ggtcccgccg atctggacgg taagctccga gccggaaacg
     3961 aattccagga tcatggaggg cccgccttgg gcggccagaa gggccggggc gagcagagcg
     4021 gccagtattg ccagtagctt gcgcgatttc atggcgtcct ccttagaact tgatcgagct
     4081 ctggaggctc gaggtgacgg tgtatccccc ggctccgtcg tatttcaggt tatagaggag
     4141 cgacaggacc gccgctccgg tacggtagtt gatctgggcg gtgatcaccg cgtcctcggc
     4201 cgtcaccagg tcctcgaaga atgttccggt cttgcccagg aagtacttct cgtagatcgc
     4261 gtcgaaggag aagcccccga cgatgccctc ccccagggtg gccacggccc gaaggtgcgg
     4321 atagtccgcg gggttgtcgg tcgcgacggc gggcttggcc gcgaaggggc cgtccaggga
     4381 ggtcttgaag gcgatcttgt cctccaggac cgagaaaccc agggaggcga accacccggt
     4441 gaaggtgtcc cccgcggggg cgacggccat caggtcgtgc ttggccgccc ggtagagatc
     4501 gtagttggcg tcgaagtaga ccgggatgaa gccgggtccc aggacccgga gctgggcgcc
     4561 gtaggtgatg aagcggaaga ggcgcccgcc cgccccgatc atggagcccc agcgtccctc
     4621 gggctggagt acgaagtccg agaaggccgc cagggggaag gtcttccccc cgaggatcgg
     4681 gatgaacacg tccagcccgt acatcgccac ggtctccagg ggctggacca cgggattgta
     4741 caggtcggcc tggaagtccg cggccgcggt caatcccacc tgcatgctct ccatgatggg
     4801 gatccccgtg ccgtagaggg gccgggcgaa gagacgggct cccaccacgt cgaagcgggc
     4861 caggttcccg gtcatcagct gggccccgac ccaggggaac tggaagaggg cgccgtcgat
     4921 gtccagggca aggccgaaga tcctctgctc cggcaggaag cgggtgttgg cgtagtcccc
     4981 cacgatgaat ccgtccccca gggtcaggtc ctggatggag ccgagcttca cgtagagggg
     5041 gtcgccccgc tgcccgtagc ggacgtacat gaacttggga aggtagacgt ccaggaaggt
     5101 cttgccgttg ccctcgtagt ccgggatcca gtcccctccg tacacggcgt cctcccccgg
     5161 ggcgggggat agcttgaagc ggagggttat gtccagcccg atgccgatct tcccgaagga
     5221 gaggtccggc tggaatccca ggcgggtcca gctggagaag cctcccagcc cgtccggcag
     5281 gacgtcggtg cccaggttga tgcccgcctg gaactggaac cccgacccgg agtcctcgga
     5341 atcctgggcg aaggcggata tggaaagaag caacaggatc gtcgcgacgg cgatcgactt
     5401 ccgcatgata aaacctcctg atctgcctga gccgggatcc aggacccgcc ggtacccgtg
     5461 cacggtatac cgacaatgcc ggagtcccac ggtaaagtat acctccccgc cgtggaaaaa
     5521 aacataccgc gccgcttgtc ccgatcccgt ctccatggta ggttcggtcg acggagcgat
     5581 gccgctaacc ctgttccccg ggagcttcca tggacttctt ttcccgtctt tccgccgcct
     5641 ggatcgagcg ccggacactc ctgtgcgtgg ggttggatcc ccggcccgcg gccggcgcgg
     5701 aggacccccg gaaggccgcg gcggagatcc tggatcgaaa ccgccgcctg atcgacgcga
     5761 ccgcggagca cgcggcctgt ttcaagccca acatcgcctt ctacgaggcc tggggttccg
     5821 cgggctggga cgccttgaag gccaccatcg actacattcc cgagtccgtt cccgtcctcc
     5881 tggacgccaa gcggggggac atcgactcca ccgcccgcgc ctacgcccgg gcggtcttcc
     5941 gggaactggg tgtggacgcg gtcaccctga gcccctacct ggggcgggac gcggcggatc
     6001 ccttcctgga gtacccggac gcggccttgt tcctgctctg ccggaccagc aaccctcggg
     6061 ccggggcctt ccaggatctg accgtggacg gcgcggcgtc ccggaccgcg gtgatttccg
     6121 ggacgggcag ggccgtgggt acggcccgga cctccgccga gcccctctac ctccgggtcg
     6181 cccgggagtg cgtgtcctgg tccgatcggg tcggcctggt cgtggcggga aacgaccccg
     6241 aggccctccg ggccgtccgt gccgcggcgc cggaggcctg gttcctggcc cccgggatcg
     6301 gcgcccaggg cggcgatatc tccgaagcct gggaggcggg tgcccgggag gacggcctgg
     6361 ggatgatccc ggccgtctcc cgggccgtct cggaagcccc ggacccggga gcggcggccg
     6421 cggccctccg cagggccgcc gcggaggcct gggagaaggc gatggaatcc cggatccacg
     6481 agcagaagcg gccggcgccc gtttccggcg tagcctcccc gacggcggta cccgggaggg
     6541 ggacagggct cgggaaccct tcggagtccg agacggggac agagctcgat cgcggaatca
     6601 tcaaggaaaa gctgctccgg ggcctggtgg agaccggatg cttccgggta ggggagttca
     6661 ccttgaagag cgggatccgc tcccccttct acatcgacct gcggagcgtg gtatccgacg
     6721 cgggcctctt ggagacggcc gcggaggcgt acgccctcct ggcccggggc ctggaatacg
     6781 accggctggg gggaataccc gcggccgccc ttcccctggc taccgcggcc gccctgcgcc
     6841 tgcggaagcc cctggtctgg ccccgcatgc ccgtgaagga acacgggacc ggaaatcggg
     6901 tggaaggccg tttccggatc ggggagaagg ttctattgtt ggatgatctg atcaccaccg
     6961 gggccagcaa gctggaggcc gcggagatcc tccggggcga aggcctcgag gtcgcggacc
     7021 tcgcggttct gatcgagcgg ggagtccggg ggcgcgggga gctggaagcg gtaggcatcc
     7081 gccttcacgc cttcctgcat gtacgggaac tgatagccct ctgcgtgtcc ctgggcctgg
     7141 tggacgcgga tgcgggtagg cgaatggagg aattcgccgc cgcggagtga ggcgcccgga
     7201 acttctccgg atgccgagga tcaggcccct cggaccatgt cctccagggt cctgtgggcg
     7261 gtgaaggacc gcaggtgcga atccatctct ctccagatct tcccggcggc gcaatcgttg
     7321 cggcgctcgc aggcatcctc tttcccgcag acgcaggggg cgattcccag gccctctccg
     7381 gaggattcca ggatggcttc cagggtgatc tccctcaggg ggcgggcgaa gtagaatccg
     7441 cctccggggc cgcgaaccga gttgatgacg ccggctttcc tcaggcggaa gaagatctgc
     7501 tccaggaatt ccggagaaat ctgctcccgc tccgagatcg ctttgatgga gaccggagag
     7561 ccgtcctcgc tcgttctggc cagggccagg acagcccgca gcgcgtacct acccttcgtg
     7621 gttatccgca attttgtgct cccgggttca ttttgcttca attatcatat cgccggagta
     7681 aggaattcgc aaggaaatta cggttgatag gataaatttt tcgtctaaag tccgatcaag
     7741 accgttccag tctccagcac cggtgcggtc gggcgtcctc gaagtccggc ggcgtggtcg
     7801 gcctggtcag atcccgcagg gatatcccgg gaaaccgggt ctcgtcgaag cggaaggatc
     7861 gggaattcgt cgagaagtag aggatgcccc ccggagaaag gacttccagg cagaggctca
     7921 cgaggtcgat ccagtcccga ttgatgtcca gggaaccgct catgcgcttg gagttcgaga
     7981 aggtgggggg atccaggatc gccaggtccc agcggcggcc ggcggcccgg gcggaggaca
     8041 ggaactccag ggcatcctca tggaaaagcg aatatgaccg gccccggaat ccgttcaggt
     8101 cgaggttgcg gccggcccag gccaggtagg tcttggaaag gtcgacgctg tccacggagg
     8161 ctgcacctcc cgcggcggca tagacggaga acgaaccggt atagcagaac aggttgagca
     8221 cccttcggtc ccgggcctcg tcccggaccc gggaccgggt gatacgatgg tccaggaaca
     8281 accccgtgtc caggtaatcc gagaggttca ccaggaagcg gagtcctccc tcttggacga
     8341 ccgccgtccg gccttcctcc gagacccgct cgtattgccc gcttccccgc tgcctcctgc
     8401 gaaccttccg gaaaatgtgc tcctccggaa cctccaggac ccgggatacc gcccggcaca
     8461 ttcggtccaa ccaggcccgt tcctccgcct cgggcttctc gtaggggcgc tcatacaggg
     8521 agacgagggc gtaggggccg taccggtcca cggccagtgg aatttccggg atatcccggt
     8581 cgtagacccg gtagcactcc acggattccc ggcgcgccca tttccggaga tggcggtccc
     8641 gtttcctcag gcggttctcg aggtattcct cctgtcggtc cggagaatcc ggcatggttc
     8701 aggcgggctg gttctttctt gtctggagca tgacggaaag cagggcgtaa cccgaggaca
     8761 ggtcttcccg atggacgacc acctcgtccg agaccttgag cttggcgttc gtgaaattcc
     8821 agatggcccg gatcccggac ttcaccaggg tatccgcggt ctcctgggcg tggggggacg
     8881 gcacggtcag gatggcgtac tccaccttgg aaccccggat cgtcgcttcc aggtccgcga
     8941 gattcaggac ggtcaccccg tggattttct tgccgatctt gtcgggatcc gtgtcgaagg
     9001 cggccacgat gtgcaggccg tggatccgga actccgggta tcccatgagg gcggagccca
     9061 ggttgcccgc gcccaccacg atggcgtcgt gcatctcgtc ccagcccagg taccgctcca
     9121 ccgcggcgat cagggcgtcc acgggatagc ctttcttggg ctttccgatg atgcccgtga
     9181 tcgccaggtc cttgcgcacc tggatcggtt ccaggttcag ttcctgggcg atcacggtgc
     9241 cggatatgta gaactggctg tttctctggg cgttgcggat gatgtggagg taggagggga
     9301 ggcgccgtat cgaaggggtg aacgatatct tcagtcgagc catgttcgcc tctttgggat
     9361 atggatgtaa aaataaatat aggggggtat agataattaa gtcaatattc aatcggaaat
     9421 aaaaggaagc cccggggaaa aaggaggttt aaaaccccgg ggctcggtgg aagcgtagag
     9481 ttatacggaa ctctacgcat aaatatacac gattcggatt ttaaatcaag agtgagtttt
     9541 gatgtcccga gtccgggatg cttccggtgc cctagtcgcg ggtgattcgg aaaggcatcc
     9601 gacgagcccg gagcccttgc aatgagaacc ggcatgtatt agaattgcgt agaatatgtc
     9661 agtatatgcg gatggttctt cattatggtc tatattgaca taaaacggga gaaggtatga
     9721 agagggcttc gagaatcctc gttttcctga tcgtgcttgt gctgcccttc gcggccgcgg
     9781 cctcggagga atccgggata gcggcccggg agacggctct ggtcatccag atcgggatca
     9841 tcctgttcgc ggtgcgcctc ggatcggccc tggcccagcg cgtcggggtg cccacggtgc
     9901 ttggggaact cctggcggga atcgtcatcg gcccctacgc cctgggcgcc ctgcccctgc
     9961 cgggattccc gacgggcctg ttcgcgggga gcatgggcga attggcggtg agcaccgaac
    10021 tctacgggat cgcgaccata gcgtccatcc tcctcctgtt cgtgtcgggc ctggagacgg
    10081 acctctccct cctcatgaag tactccctcg cggggggcat catcgggatc gggggcgtgg
    10141 cgctctcctt cgcggccggg gacctcctgg gcatggtcct gttccggacc ggtgccatgg
    10201 atccccgggc cctcttcctg gggatcatgt ccaccgccac cagcgtgggg atcacggcgc
    10261 ggatcctctc ggaccgcagg aagatggaca gccccgaggg cgtgaccatc ctggcggccg
    10321 ccgtcttcga cgacgtcctg ggcatcgtct tcctggcggc ggtcatgggg atcgcgaccc
    10381 tgacccggga cgggacggga ggccccctgc cctggggctc catcctgggg attgcgggga
    10441 gggccttcgg cttctggctg gggttcacgg ccctgggcct gttgctgtcc aagcagctgg
    10501 cccgtttcct caagagtttc cggaatccgg cctacttctc catcctggcc ctgggcttgg
    10561 ccctgatcct ggccgggatc ttcgagaagc agggcctggc catgatcatc ggggcctacg
    10621 tcctgggcat ctccctgtcc aagtccgaca taagccatgt catcctggac aagacgcggg
    10681 gcctctacga gttcctggtg cccctgttct tcgcggtgat gggaatgctg gtcaacgtcc
    10741 gacagctcct ggacccggcg gtcctcctgg gaggcctcgc gtactccctc gtgtcggtcg
    10801 cctcgaagtt catcggctgc ggatttcccg ccctgttcct gggcttcaac ggcaagggag
    10861 ccctccgcat cggcgccggc atggtgcccc gcggggaagt ggccctcatc gtggccggga
    10921 tcgggttgtc cgggggcctc ctgagcgaaa acgagttcgg catggccata ttgatgaccc
    10981 tgatcactac gatcgccgcc cccccggtcc tttccgcggc cctgcggatt cccggtcggg
    11041 gtacccgggc ggtggagaag ggggccgagg cggagtcctc cgtctacgcc ttcccctccg
    11101 aggacgtcgc ggtcctggtt tcggacgtcc tggtccggga tctccagaac gagggcttct
    11161 tcgtccagct catgaacgtg ggggacggga tcgtgcaggt ccgcaaggac gacatcgcct
    11221 tctccctgga gcgcaagggg gtcgagctga ccctgcggat ggatccttcg gacgcgatct
    11281 tcgtgaagac cgccctgttc gaggccgtgg cccagctcaa ccaacgcttc gaggaactcc
    11341 ggaaaaacct ggaccccaaa cggcttaccg agggcctgga cgaggagggg gggcgcgtga
    11401 atcccgggtt ccgggaagct ctgtccccgg actgcgtcat cctgaacctc aagggagcgg
    11461 acaaggagtc cgtcatcacg gagctcgtgg accgcttgag ttcgaaaggg ctcctggcgg
    11521 atcgggccca ggtcctgcgg gacgtcctgg accgggagaa gtccatgagc acggggatgc
    11581 agcacggcat cgcccttccc cacgccaaga ccgagggggt gacccgcatc gtggcggccc
    11641 ttggcctgaa acccgagggc gtcgagttcc agtccctgga cggcgcgccc gccaaggtca
    11701 tcctcctcct ggcttccccc ggcaggcagg cgggccccca tatccaggtc ctggcctccg
    11761 cggccgccct cctgaacgac gagatccgcc ggacggacct gctcgcggcc cagagcgtgg
    11821 aggaggccgc ggtgatcctg ggcctgggga ccaagaaagc cgtgtccagg gattagcagg
    11881 gcgctgacaa actcgaagct agtcagcacc ctgccatgca attgctcgat aaacttgcgc
    11941 ttttctgcgc aattgcgaca ttaaggaggc tgtcgacaaa ggcgccatcg cccttgtcaa
    12001 cagcctgtta ggctcgggcg ccccggctca ttcccaccag gtccgggcgg tgaagtccgg
    12061 ccggatggag gctccc
//
LOCUS       DAOTEI010000103        12035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3712_length_12035_cov_6.330968, whole genome shotgun sequence.
ACCESSION   DAOTEI010000103 DAOTEI010000000
VERSION     DAOTEI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3712_length_12035_cov_6.330968"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="P5313_10590"
     CDS             <1..1808
                     /locus_tag="P5313_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY80852.1"
                     /translation="DVVELLRGGTCDLSDPGRASGLVVIAGLPWLVAVSPVLPTGGNG
                     TPRGLMAMARRIDDRFVGTLSGILGLPVSVTPEEEPPVLEPEPRAFPERALPRPESGE
                     PAWTAAEILDRRTIAGYARVEGYAGFPGVRIRILEPRTIRSVGIRSYVYFAAALGLLI
                     SAAAISQLVLFQRLVVERIVRLGRDLDLLDTRDDPGIRMTVAGNDELSRFASALNAAL
                     DKLAAARSRLEQARRGEALADLAGGMAHEFNNILAIILGALESAGTRGRTEEERVRLL
                     GTARTAALRGRDIVDQVLLHARTARTERRPADLPRVLDEALEQVAVLAKPGIEFRRRY
                     APMPPVPVNKGLVTMLVFNVVKNALDAIGAGPGTVSVSLDPEEVGPGTGEGNRPPPGR
                     YARLSLEDSGRGMDPQELAHAFDPFFTTKSPGRGLGLGLAVVKGVMRQHDGHIDVSSE
                     PGKGSRFRFYFPLGTPGGGLPPEPEGARSRDTPPPSGRLLLVGTDPALALAERGLGML
                     GYAVHRAEDEAAASDRLNRGVDDIDLVIADEGIGGVGLEALARAARGSGRLRFLVCAA
                     PGSGASPGSGVHAVLAKPVPIRSLIAAVEEALSTP"
     gene            1903..2292
                     /locus_tag="P5313_10595"
     CDS             1903..2292
                     /locus_tag="P5313_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY80853.1"
                     /translation="MAIEVLLIEDDSTLAELLGSALSEAGFAVRTAADGAEGLRVFTE
                     RPSEVVVTDILMPRMEGYETIIELKAMNPGVRILAISGGGVTGRGEDILRHASMFGAH
                     AVMKKPISLEDFVSKVRDLAARDACIG"
     gene            complement(2335..3255)
                     /locus_tag="P5313_10600"
     CDS             complement(2335..3255)
                     /locus_tag="P5313_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRY80854.1"
                     /translation="MIAAVCTRYGPPEVLRLVELETPVPRKNEVRIRIAATAVTSSDT
                     YVRGLLLTPAYRMMARLALGWTAPRRPVLGMVLSGVVDSVGAQVRLFKAGDRVFGFNP
                     YRFGAYAQYVCWPEDGLLAVRPVNLGDEEAAALPYGGLLAYHFLRKAGVLAGRRILVF
                     GASGAVGTSAVQIARHLGAEVTGVCGGANADLVASLGAERVVDYGVEDFTERAERYDL
                     IFDAVGRRKSAAALRSCRRALAPGGACVSVDDGKPKLRREDLVSLGELAAAGRIKPVI
                     DRTYALADIVEAHRYVEEGHKRGNVIVRVP"
     gene            complement(3252..3947)
                     /locus_tag="P5313_10605"
     CDS             complement(3252..3947)
                     /locus_tag="P5313_10605"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HRY80855.1"
                     /translation="MNRDRNRAAAAGALFIVATTASLLGRAVLLDPILEGVGFLAEVA
                     ANQDRTAAGALLGLVAYFASASIAIALYPVLRRYGEGLALGSVGLRLVEGVLYSLGAI
                     AVLASGTLARGLAEADTATRVSLQASGDLLMAVRSWAGMAGTLAFYPAGLLYYLVFFR
                     SRLIPRWLSGWGIVAVAMGFTASILVVFHIILPMSAPQIVLNFPIFLQEMVLAVWLIA
                     RGFSRPAASEDAR"
     gene            4073..4789
                     /locus_tag="P5313_10610"
     CDS             4073..4789
                     /locus_tag="P5313_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY80856.1"
                     /translation="MSRGSVPGAGTPPGGLIIVRSTHTTESRDTLDRERVLHAAMELA
                     DEAGIESVTMRELGRRLGVKAASLYNHIAGKDDLLDGLMNLALSGIDIALDGLDWKDA
                     MRRRAVSAREVFARHPWAAGLIDSRDRRGRESLAYVDRVLGVLIRAGFSTADAANAFL
                     VLDSYIYGFERQRPAAPEGNGTDGTGEAREILAAIPEGEYPFAARVAAEFSGKPFDRE
                     AAFEFGLGLILDGLERSLGH"
     gene            complement(4849..5658)
                     /locus_tag="P5313_10615"
     CDS             complement(4849..5658)
                     /locus_tag="P5313_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRY80857.1"
                     /translation="MRTAILGAGRMGSWLAETLAPAHEVAVRDTESGRAERVSGVKVL
                     RTDGELLDFAPELLVNCVPLGFTIDVFRRTLPLLPETCMLSDIASVKTGFGEYYRETG
                     RPFVSTHPMFGPTHADVRSLEGENAVVITESCEEGKEFFRNLYRGLGLRVFEDSFDGH
                     DRTVAYSLATPFASTLVFSACMKNLDAPGTNFRKHLEIARSLLGEDDRLLAEILFNPH
                     TVRQIELINSQLAYLNHIIMGRDFEEMQKFLARLRSNLGMAGEVSSGTGGI"
     gene            complement(5660..6712)
                     /locus_tag="P5313_10620"
     CDS             complement(5660..6712)
                     /locus_tag="P5313_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HRY80858.1"
                     /translation="MTLERLRADIDRIDARILSLLEERMEKAILTRRLKPGTRDPARE
                     AEILARVAARSRCLAREEFTTALYGSILAESRAIQDASPRLVGFQGTHGAYSESAVRA
                     WDPAAAAIPFPEFGQVFDGVRNGSLDFGVVPVENTLGGIVGQVNSILVTTELRVVAAV
                     DLPVAHCLLVAPGQDHREIRTAYSHPQALAQCRRFLERNKLDPVEHFDTAGAARMIAE
                     DRPKGAAAIAGRFAAELYGLEIIKEGIQDAQNNRTRFFVLSREAPEAGGNKCSAVFFT
                     DDKAGALFRVLEVFARSGINLTRIESVPNEPGDYAIFLDFEGSDRDPGVARAVSEASA
                     LARDFRMLGCYTEARL"
     gene            complement(6763..7764)
                     /locus_tag="P5313_10625"
     CDS             complement(6763..7764)
                     /locus_tag="P5313_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRY80859.1"
                     /translation="MNTTGRIFQVSLFGESHGPAVGVTLDGVPPGLALAPEDFEADLA
                     RRRSGAAGTTDRREPDRPEILSGLYRGRTTGTPLCLIFRNTDTRSEDYAPLASVPRPG
                     HADFTGSVRYRGFQDPRGSGHFSGRITAGLVAAGVVAKKLLPGVALETRILEAGGDTD
                     VQAAARRASEAGDSVGALVEVRVRGLPAGLGEPFFDPVEGRIAGALFSVPGVRGVEFG
                     DGFAAARMLGSGHNDPIVGKDGRTSRNGAGGANGGITNGNDLLVRIAVKPASSIRMPQ
                     RTLDFTTGEMTDLSVPGRHDACIALRAAVVLEAACAVCLADFILLARTYDAAPDIEG"
     gene            complement(7761..8942)
                     /locus_tag="P5313_10630"
     CDS             complement(7761..8942)
                     /locus_tag="P5313_10630"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HRY80860.1"
                     /translation="MSPLGGAAAGPASPGAVPDILLTTEAGDSRILFSPAARLPDLAA
                     PDRTVVVTDGLVRRHVPTAYPADRIVEVPRGEAAKSLSCLESVYRRFLELGLGRDGTV
                     LAVGGGSVSDLAGFAASTWMRGLDFGFAPTTILSMVDASVGGKNGIDFGGRKNLVGSF
                     RLPRFVLVDPSVLSTLPARQAAGGLVEAVKHAVIEGPEHLDLLERAIPARPAGAAFRL
                     PSDPALLAEVLERSIRMKARIVESDFRESGERRKLNLGHTIGHAVEAVTGLPHGECVA
                     AGLACALGLAARRGGCGEDARRILDLLGRLGVPRSLEEARMAALEDAAAAPDGLGGPG
                     SAETLADPAAFRNAVARALSADKKRSGSVVLFALPRAPGSVEIEPIGLAELEDFVREA
                     P"
     gene            complement(8939..9781)
                     /gene="aroF"
                     /locus_tag="P5313_10635"
     CDS             complement(8939..9781)
                     /gene="aroF"
                     /locus_tag="P5313_10635"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRY80861.1"
                     /translation="MKNLQLAGAKEPGARTVIEVRGVRLGEEFVVVAGPCAVESEDQV
                     VRTAVAVKEAGAKMLRGGAFKPRTSPYSFQGLGLKGLKILDKARRETGLPIITEVIDT
                     RDVSWVGEYADVLQIGARNMQNFSLLREAGKSGRPVLLKRGMYSTLEEWLNCAEYILA
                     EGNPNVILCERGIRTFETYTRNTLDLSIVPAVKMVSHLPVLVDPSHGTGVLGLIEPMS
                     LAALVAGADGLEIEVHIDPPSAWSDKDQQLTPEAFGRLMRKLEILRETMAGLDARAAE
                     TAAP"
     gene            9896..11110
                     /locus_tag="P5313_10640"
     CDS             9896..11110
                     /locus_tag="P5313_10640"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY80862.1"
                     /translation="MPGLSERTRTLEPSRTIAMNQRARDLAASGLDIADLTVGEPDFH
                     PPENVRAAAARAIAEGADRYTPVAGYPELREAVSRKLLRENGLSYPASQVMVSCGAKS
                     CLSNAVQALVGPGDEAIIPVPAWSTYAEIVKLAGGVPVLVPTSAAEGYKLRPERLASA
                     LTSRTRLLVLCSPSNPTGAVFTRPELEALAAVLERFPETWVLSDEVYERIRYVPEVPS
                     PAAIPSLADRCVTVNGLSKSHAMTGWRIGFLAGPRPLVDACIALQGQTTTCASSISQK
                     ASLAALETEPAVIDAFVAAFARRRERAAAALRSIPEVSLVDPDGAFYLFPDLSAFLGL
                     PWKDRALESSDDLALFLLEEARAAFVPGSAFGPGAEGCVRISFAASDERIDAALGRTR
                     AALEGLRAGAFA"
     gene            complement(11448..>12035)
                     /locus_tag="P5313_10645"
     CDS             complement(11448..>12035)
                     /locus_tag="P5313_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY80863.1"
                     /translation="NALIASIAAQTNLLAMNAAIEAAHAGEAGRGFAVVADEIRKLAE
                     NAARQSKAISGDVREISGAIAAVVSSADESAARFSALVDKIEEIRRVEEGILAAMAEQ
                     SAGSAQVLEALNQIHEITDEVRAGAGEMREGADAVIGEMKRLLDASVEIEHSMTEIAS
                     GAAEVSQASNLTADLTVRNRDGIGAVTERMGRFKV"
BASE COUNT         1819 a         4375 c         4068 g         1773 t
ORIGIN      
        1 gggacgtggt cgagcttctc cggggcggga cgtgcgacct gtccgatccc gggcgtgcga
       61 gcggcctcgt ggtgatcgcc ggcctcccct ggctggtcgc cgtctcgccc gtgcttccca
      121 cgggagggaa cggcactccg cggggcctca tggccatggc ccgtcggatc gacgaccggt
      181 tcgtggggac cctgtccggc atcctgggac ttccggtgtc cgtcaccccc gaggaggagc
      241 cgcccgtcct cgaacccgag ccccgggcct tcccggaacg agccctcccg cgcccggagt
      301 ccggcgagcc ggcgtggacg gcggccgaga tcctcgatcg gaggaccatc gcggggtacg
      361 cacgggtgga ggggtacgca ggcttcccgg gagtccggat ccgaatcctg gagccccgca
      421 ccatccggtc cgtgggaatc cggtcctatg tgtacttcgc ggcggccctc gggctcctca
      481 tatcggcggc ggcgatctcc cagctcgtcc tgttccagcg cctcgttgtc gagcggatcg
      541 tccggctcgg ccgcgacctg gatctcctgg acacccgcga cgatcccggc atccggatga
      601 cggtcgccgg aaacgacgaa ctctcgcggt tcgcttcggc cctcaacgcg gccctcgaca
      661 agctggccgc cgcccgttcc cggctggagc aggcccgccg cggcgaagcc ctcgccgacc
      721 tggccggggg gatggcccac gaattcaaca acatccttgc gatcatcctg ggcgcgctcg
      781 agtcggcagg cacccggggc cggaccgagg aggagcgcgt gaggctcctc gggaccgccc
      841 gcacggcggc gctccgggga cgcgacatcg tcgatcaggt gctcctgcac gcccgcacgg
      901 cccggacgga gcgccggccc gccgacctcc cgcgcgtcct ggatgaggcc ctggaacagg
      961 tggccgtgct ggccaagccc ggcatcgagt tccgacgccg ctacgccccg atgccgccgg
     1021 ttcccgtcaa caagggtctc gtgaccatgt tggtgttcaa cgtggtgaag aacgccctgg
     1081 acgccatcgg cgcaggaccg ggaaccgtgt cggtgtccct ggacccggag gaagtcggac
     1141 ccgggaccgg ggaggggaat cgaccgcccc ccggccggta cgcccgactc agcctggagg
     1201 actccggccg ggggatggat ccccaggaac tcgcccacgc cttcgatccc tttttcacca
     1261 ccaagtcccc gggcaggggc ttggggctgg gcttggccgt ggtcaagggt gtcatgcgtc
     1321 agcacgacgg ccatatcgac gtgtccagcg aaccggggaa gggatcccga ttcaggttct
     1381 actttcccct cgggactccc ggcggaggac ttcctccgga acccgaaggc gcgcggtccc
     1441 gggatacgcc ccctccctcg ggcaggctgc tcctggtcgg gacggacccg gccctcgccc
     1501 tcgccgaacg gggactcggc atgctaggct atgccgtgca ccgggccgag gacgaggcgg
     1561 ccgcgtccga tcgcttgaac cgtggagtcg acgatatcga tctcgtaatc gccgacgagg
     1621 gaatcggggg ggtcggattg gaggccctcg cccgggccgc gcggggctcg gggcgtctcc
     1681 ggttcctggt gtgcgcggct cccggctccg gtgcgagtcc gggttccggc gtccacgcgg
     1741 tgctggcgaa accggtgccg atccggtccc tgatcgccgc cgtcgaggag gccttgtcga
     1801 cgccttagtc agggtcctgt cgacaacgac gccgccgacc ttgtcgaaag cctgttttgc
     1861 gcggcggccg acccgagacg tcgagccgcc cctggagtga ccatggccat cgaagtcctg
     1921 ctgatcgaag acgactcaac cctggccgaa ctgctgggct ccgcgctctc ggaggccgga
     1981 ttcgccgtcc ggacggccgc ggacggcgcc gaaggcctcc gggtcttcac cgagcggccc
     2041 agcgaggtgg tggtgacgga catcctgatg cccaggatgg agggctacga gaccatcatc
     2101 gaactgaagg cgatgaaccc cggggtgagg atcctcgcga tctccggcgg gggcgtgacc
     2161 ggccgcggcg aggacatact ccgccacgcg tccatgttcg gagcgcacgc ggtcatgaag
     2221 aagcccattt ccctggaaga cttcgtctcc aaggtccggg acctcgccgc ccgggacgcc
     2281 tgcatcggat gacccttccg ggatcacccc cgggggacgg aggccgtcgg agcgtcacgg
     2341 tacgcggacg atcacgttgc cgcgcttgtg cccctcttcg acgtacctgt gcgcctcgac
     2401 gatgtccgcc agggcgtagg ttcgatcgat cacgggcttg atccttcccg cggcggccag
     2461 ctcgcccagg gagaccaggt cctcacgtcg aagtttcggc ttaccgtcgt cgacggagac
     2521 gcaggccccg ccgggggcga gcgcccgacg gcagctccga agggcggcgg cgctcttccg
     2581 cctgcccacg gcatcgaaga tcaagtcgta gcgctccgcc ctctccgtaa agtcctcgac
     2641 cccgtagtcg accactcgtt cggcgccgag ggacgcgacg aggtccgcgt tcgccccgcc
     2701 gcagactccc gtgacctccg caccgagatg ccgggcgatc tgcaccgccg acgtgcccac
     2761 ggccccggag gccccgaata ccaggattcg ccgtcccgcc aagaccccgg cctttcgaag
     2821 gaagtggtac gccaggagac ccccgtaggg aagcgcggcc gcctcctcgt cgccgaggtt
     2881 gacgggccgg acggccaaga gcccgtcttc gggccagcag acgtactggg cgtaggcgcc
     2941 gaaacggtac gggttgaacc cgaacacgcg atcccccgcc ttgaacaaac ggacctgggc
     3001 tccgaccgaa tccaccaccc cggagagcac catgccgagc acgggccgcc tgggagcggt
     3061 ccacccgagg gccagccgcg ccatcatccg gtacgccgga gtcagcagga ggccccggac
     3121 ataggtatcg ctcgacgtca cggcggtcgc cgcgatgcgt atacgcacct cgttcttccg
     3181 ggggacggga gtttcgagct ccacgagccg gagcacttcg ggcggtccgt agcgggtgca
     3241 gaccgcggcg atcatctcgc atcctcggag gcggcgggcc gactgaatcc ccgggctatc
     3301 aaccacaccg ccagcaccat ctcctgcagg aagatcggga agttcaggac gatctggggc
     3361 gccgacatgg ggagaatgat atgaaagacg accaaaatgg aggctgtgaa gcccatcgcc
     3421 accgcgacga tgccccagcc ggacagccag cggggaatga gccgggatct aaaaaacacg
     3481 aggtagtaca ggagtcccgc cgggtagaag gccagggtac ccgccatgcc cgcccagctt
     3541 cggaccgcca tcaacaagtc gcccgaggct tgcagggaaa cccgggtcgc cgtgtccgcc
     3601 tccgcgagtc cccgggccaa ggttcccgac gccagcacgg ctatggcgcc caggctgtag
     3661 agcaccccct cgacgagtct caaccccacg gaaccgagcg ccagcccctc gccgtaccgc
     3721 ctcaggacgg gatacagcgc gatcgcgatg ctcgcggacg cgaagtacgc gaccagcccc
     3781 aggagcgccc ccgcggccgt ccggtcctgg ttcgcggcga cctcggcgag gaagccgacc
     3841 ccctcgagga tcggatccag cagcaccgcc ctgccgagga ggcttgccgt cgtggcgacg
     3901 atgaagagcg cccccgcggc tgccgcccga ttccggtctc tattcatgtg gttctctcct
     3961 gattccgagt cctcgtccgc ccctccgtcc cctgcaggcg ccccggttcg gaacacccga
     4021 tactaacagt gttagcatcg ggtgttgcca agcatgtact aacagtgtta gtatgtcaag
     4081 gggttccgta cccggcgcgg gaacaccccc gggaggtctg atcatcgttc gctcgacaca
     4141 tacaacggaa tcgcgggaca ccctggatcg ggagcgcgtc ctgcacgcgg ccatggagct
     4201 cgcggacgag gccgggatcg aatcggtcac catgcgagag ctcggccgaa ggctgggggt
     4261 caaggcggcg tcgttgtaca accacatcgc cggcaaggac gaccttctcg acggcttgat
     4321 gaacctcgcc ctgtccggga tcgacatcgc cctggatggt ctcgactgga aggacgcgat
     4381 gcggcgccgg gcggtctcag cgcgggaagt cttcgcacgt cacccctggg ccgcgggtct
     4441 gatcgactcc cgcgaccgga ggggtcggga gagcctggcc tacgtggacc gcgtcctggg
     4501 cgtgctcatc cgggccgggt tttcgaccgc ggacgcggcg aacgcgttcc tcgtcctcga
     4561 cagctacatc tacggcttcg agcggcagcg gcccgccgcc ccggagggaa acgggacgga
     4621 cggcaccggg gaagcccggg agatcctggc ggccatcccg gagggcgagt accccttcgc
     4681 ggcccgcgtc gcggcggagt tctccgggaa gcccttcgac cgggaggccg ccttcgagtt
     4741 cggcctcggg ctgatcctcg acggcctgga gcgttccctg ggacactgac ccggacgcgc
     4801 gaggcggaag ctgcccggga acggcggcgg cccgggcgcc cggatatcct agatgccccc
     4861 ggtcccggag gaaacctctc cggccatccc caggttggat cggagccggg ccaggaactt
     4921 ctgcatctcc tcgaaatccc gccccatgat gatgtggttc aggtaggcca gctgggaatt
     4981 gatgagctcg atctgccgga ccgtatgcgg gttgaacagg atctccgcca ggagacgatc
     5041 gtcctccccg agcagggacc tggcgatctc caggtgcttg cggaagttgg tgcccggggc
     5101 atccaggttc ttcatacagg ccgagaagac cagggtggag gcgaaggggg tggccaggga
     5161 gtaggccacg gtccggtcgt ggccgtcgaa gctgtcctcg aaaaccctaa ggcccaggcc
     5221 ccgatagagg ttccggaaaa attcctttcc ctcctcgcag gattccgtga tgaccaccgc
     5281 gttctcccct tccagggacc gcacgtcggc atgggtcgga ccgaacatgg ggtgggtgga
     5341 aacgaagggt cgcccggtct cccggtagta ctccccgaag ccggtcttca cggaggcgat
     5401 gtccgagagc atgcaggttt cgggcaggag gggcagggtc cggcggaaca cgtcgatcgt
     5461 gaatcccagg ggcacgcagt tgaccaggag ctccggcgcg aagtccagga gctccccgtc
     5521 ggtccggagg accttcaccc cggacacccg ctccgcccgg ccgctctccg tatcccgcac
     5581 cgccacctcg tgggcggggg ccagggtttc cgccagccag gagcccatgc gcccggcgcc
     5641 gagtatcgct gtcctcatgt caaagcctcg cttcggtgta gcagcccagc atccggaagt
     5701 cccgggcaag ggcagacgcc tccgacacgg cccgggctac gcccgggtcc cggtcggagc
     5761 cttcgaagtc caggaagatc gcgtagtccc cgggctcgtt cggcacggac tcgatccggg
     5821 tcaggttgat ccccgaccgg gcgaagacct ccaggacccg gaagagggcc ccggccttgt
     5881 cgtccgtgaa gaagaccgcg gagcacttgt tcccccccgc ctccggcgcc tcccgggata
     5941 gcacgaagaa ccgggtccgg ttgttctggg cgtcctggat gccctccttg atgatctcca
     6001 ggccgtaaag ctccgcggcg aagcgccccg cgatagccgc ggcccccttg ggccggtcct
     6061 cggcgatcat gcgggcggcc ccggcggtgt cgaagtgctc caccgggtcc agcttgttcc
     6121 gttccaggaa gcggcggcac tgggccaggg cctggggatg ggagtaggcg gtccggatct
     6181 cccggtggtc ctgccccgga gccaccagga ggcagtgggc cacgggcagg tccaccgcgg
     6241 ccaccacccg gagctccgtg gtcaccagga tggagttcac ctggcccacg atgcccccca
     6301 gggtgttctc cacggggacc accccgaagt ccaggctccc gttccggacc ccgtcgaaga
     6361 cctgtccgaa ctccgggaag ggtatggccg ccgcggcggg atcccaggcc cggaccgcgg
     6421 actcgctata ggccccgtgg gtgccctgga agccgaccag ccggggggag gcgtcctgaa
     6481 tagcccggct ctcggccagg atcgagccgt agagggccgt ggtgaactcc tcccgggcca
     6541 ggcaccggga ccgggcggcc acccgggcca ggatctccgc ctcccgggcg ggatcccggg
     6601 tgccgggctt gagcctgcgc gtgaggatcg ccttttccat gcgctcctcc agcagggaca
     6661 ggatgcgtgc gtcgatgcgg tcgatgtccg ccctcagccg ttccagggtc atgtcgcttc
     6721 ctttcggcgg gtctcccggt tccccggggc gcccgtccgg tcctatcctt cgatatccgg
     6781 ggccgcgtcg taggtccggg ccagcaagat gaagtccgcc aggcagaccg cgcaggccgc
     6841 ctccaggacc accgcggccc ggagggcgat gcaggcgtcg tggcgccccg gcacggagag
     6901 gtccgtcatc tccccggtcg tgaaatccag ggtcctctgg ggcatgcgga tcgaggaggc
     6961 gggcttcacg gcgatcctaa ccagcaagtc gttgccgttc gtgatacccc cgttggctcc
     7021 gccggccccg ttccgggagg tccggccgtc cttgccgacg atcgggtcgt tgtgcccgct
     7081 cccgagcatc cgggccgcgg cgaagccgtc cccgaactcc accccccgga ccccgggcac
     7141 ggagaatagt gccccggcga tcctgccctc cacggggtcg aagaagggct ccccgagccc
     7201 cgcgggaagc ccccggaccc ggacctccac cagggccccc acggagtccc cggcctcgga
     7261 cgcccggcgg gcggcggcct ggacgtcggt gtcccccccc gcctccagga tccgggtctc
     7321 caaggcgacc ccgggcagga gtttcttggc caccaccccc gcagcgacga gccccgcggt
     7381 gatgcgcccg gagaaatgcc cgctcccccg ggggtcctgg aagccccggt accggacgga
     7441 acccgtgaag tccgcgtgcc ccggcctagg gaccgaggcc aggggggcgt agtcttcgga
     7501 ccgggtgtcc gtgttccgga agatcaggca gaggggggtg cccgtggtcc ggccccggta
     7561 gaggccggag aggatctccg gccggtccgg ctcccgccgg tccgtggtgc ccgcggcccc
     7621 ggagcggcgg cgggccaggt ccgcctcgaa gtcctcgggc gccagggcca gcccgggggg
     7681 taccccgtcc agggtcaccc ccacggcggg gccgtgggac tccccgaaga ggctcacctg
     7741 gaagatccta cctgtcgtgt tcacggcgcc tcccgtacga aatcttccaa ttcggcgagc
     7801 ccgatcggct cgatttccac ggatcccggg gcccggggca gggcgaaaag caccacactc
     7861 ccggaccgct tcttgtccgc ggacagggcc cgggcgacgg cattgcggaa ggccgcgggg
     7921 tccgcgaggg tctcggccga accgggaccg ccgaggccgt ccggagcggc ggccgcgtcc
     7981 tcaagggccg ccatccgggc ctcctccagg gacctcggca cgcccaggcg tccgaggaga
     8041 tccaggatcc tgcgggcatc ctctccgcag cctccgcgcc gggccgcgag tcccagggcg
     8101 caggccagtc ccgcggccac gcactccccg tggggcaggc ccgtgacggc ctccacggcg
     8161 tgcccgatcg tatggcccag gttgagcttg cgccgttccc cggactcccg gaagtccgac
     8221 tccacgatcc gggctttcat gcgtatcgag cgttccagga cctccgccag gagggccggg
     8281 tccgagggaa gtcggaaggc cgcacccgcg ggccgcgcgg ggatcgcccg ctccagcagg
     8341 tccaggtgct ccggcccctc gatcacggcg tgcttgaccg cctccacgag gccccccgcg
     8401 gcttgcctgg cgggcagggt cgaaagcacg gagggatcga ccaggacgaa gcggggaagg
     8461 cggaaggatc cgaccaggtt cttgcggccc ccgaagtcga tcccgttctt gccccccacc
     8521 gaggcgtcta ccatggaaag gatcgtggtg ggtgcgaagc cgaagtccag cccccgcatc
     8581 caggtggagg ccgcgaaccc ggccaggtcc gagacggatc cgcccccgac cgcaaggacg
     8641 gtcccgtccc ggcccagtcc gagctccagg aaacgccggt agaccgactc caggcaggaa
     8701 aggctcttgg cggcctcccc cctcgggacc tccacgatcc ggtccgcggg ataggcggtc
     8761 gggacgtggc ggcggacgag gccgtccgtc accacgacgg tccggtccgg ggcggccagg
     8821 tccgggaggc gtgccgccgg ggagaacagg atccgggagt cccccgcctc ggtcgtaagg
     8881 aggatgtccg gaacggcgcc gggggacgcc ggacccgcgg ctgccccgcc cagagggctc
     8941 atggggccgc ggtctccgcc gcacgggcgt ccaggcccgc catggtctcg cgcaggatct
     9001 ccagctttcg catgagccgg ccgaatgcct cgggggtcag ctgctggtcc ttgtcgctcc
     9061 aagccgaggg cgggtcgatg tggacctcga tctccaggcc gtccgccccg gccaccaggg
     9121 cggccaggct catgggctcg atcaggccca ggacgccggt gccgtgggag gggtccacca
     9181 ggacgggcag gtggctgacc atcttgaccg ccgggacgat ggagaggtcc agggtgttcc
     9241 gggtgtaggt ctcgaaggtg cggatccccc gctcgcagag gatgacgttg gggttcccct
     9301 ccgccaggat gtactccgcg cagttgagcc actcctccag ggtggagtac atcccccgct
     9361 tgaggaggac cgggcggccg gacttgcccg cttcccggag cagggagaag ttctgcatgt
     9421 tccgggcccc gatctgcagg acgtccgcat actcccccac ccaggacacg tcccgggtgt
     9481 cgatcacctc ggtgatgatg ggaagccccg tctcccggcg ggccttgtcc aggatcttga
     9541 ggcccttgag tcccaggccc tggaaggaat agggcgaggt ccggggcttg aaggccccgc
     9601 cccggagcat cttggccccc gcttccttga cggccacggc ggtccggacg acctggtcct
     9661 cggactccac ggcgcagggg ccggccacca caacgaactc ctcccccagg cggacccccc
     9721 ggacctcgat cacggtccgg gccccgggct ccttggcccc ggccaactgc aggttcttca
     9781 tcccgatcct ccgccggaat ttcccggcac catccttgca taatcgtccg cacgaccgta
     9841 tacactaagc ccgggcggac cggcctgaac cgaagtccgc cggaaggacg gccccatgcc
     9901 cggactctcc gaacgcaccc ggaccctgga accctcccgc accatcgcca tgaaccagcg
     9961 agcccgcgac ctggccgcct cgggcctgga catcgcggac ctcacggtcg gggagccgga
    10021 cttccacccc cccgagaacg tacgggccgc cgcggcccgg gccatcgccg agggggccga
    10081 ccgctatacc cccgtggcgg gttaccccga gctccgggaa gccgtatccc ggaagctcct
    10141 ccgggagaac ggcctatcgt atcccgcctc ccaggtcatg gtctcctgcg gggccaagag
    10201 ctgcctttcc aacgccgtcc aggccctcgt cggccccggg gacgaggcta tcatccccgt
    10261 cccggcctgg tccacctacg ccgagatcgt gaagctggcc gggggtgtcc cggtcctcgt
    10321 cccgacctcc gccgcggagg gctacaagct ccgccccgag cgactggcct cggccctcac
    10381 ctcccgcacc cggctcctcg tcctctgctc cccctcgaac cccaccggcg cggtcttcac
    10441 ccgcccggaa ctcgaagccc tggccgccgt cctggagcgc tttccggaga cctgggtcct
    10501 ctcggacgag gtctacgagc gcatccgcta cgtccccgag gtcccgagcc ccgcggcgat
    10561 cccctccctg gccgaccgct gcgtcacggt caacggcctg tccaagtccc acgccatgac
    10621 gggctggcgg atcggcttcc tggccggacc ccgtcccctg gtggacgcct gcatagccct
    10681 ccagggccag acgaccacct gcgcctcctc catctcccag aaggcctccc tggcagccct
    10741 ggaaacggag cccgccgtga tcgacgcctt cgtggccgcc ttcgcccggc gcagggaacg
    10801 ggccgccgcg gccctccgct cgatccccga ggtctccctc gtggaccccg acggcgcctt
    10861 ctacctcttc ccggacctct ccgccttcct gggccttccc tggaaggacc gcgccctgga
    10921 atcctccgac gacctggccc tcttcctcct ggaggaagcc cgggccgcct tcgtgcccgg
    10981 ctccgccttc ggccccgggg cggagggctg cgtccgcatc tccttcgccg cctcggacga
    11041 acggatcgac gcggccctgg ggcggacccg ggccgccctg gagggcctgc gggccggagc
    11101 cttcgcctag gggccgggcg ctcccgggcg cgccaccgga atacacctcg ccatcggccg
    11161 gtctaccggg gctcaccccg cccggccgtg ccgcgaagga gccctgctag cgatagagga
    11221 gataaaagta aaaggaatag ggatgggatc gacaggggga tgaggcggtt ggattgttgg
    11281 cgtcccggcc gctgtcgcgg cgggcggcgg atccttccgg tgccatgccc gggaagctag
    11341 cacgccggga aaaaagaatc aagttacttt acggagaaac tttcgaaacc cgaaacggtt
    11401 cgccccctta caggttgttg aaatgcctcg agcatttcga caacctgcta gaccttgaat
    11461 cggcccatcc gctcggtcac cgcgccgatc ccgtcccggt tccggacggt caggtccgcg
    11521 gtcaggttgg aagcctggga gacctccgcc gccccggaag cgatctcggt catgctgtgc
    11581 tcgatctcca cgctggcgtc caggaggcgc ttcatctccc cgatcacggc gtccgccccc
    11641 tcccgcatct ccccggcccc ggcccgcacc tcgtcggtga tctcgtggat ctggttcagg
    11701 gcctccagga cctgggcgga tccggcagac tgctccgcca tggccgccag gatgccctcc
    11761 tcgacgcggc ggatctcctc gatcttgtcc accagggccg agaaccgggc ggcggattcg
    11821 tccgcggaac tcaccaccgc ggcgatggcc ccggagatct cccggacgtc cccggagatg
    11881 gccttggact gccgggcggc gttctccgcg agcttgcgga tctcgtcggc caccaccgcg
    11941 aagcccctcc cggcctcgcc ggcgtgggcc gcctcgatgg cggcgttcat ggccaaaagg
    12001 ttggtctggg ccgcgatgga agcgatgagg gcgtt
//
LOCUS       DAOTEI010000104        11905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3772_length_11905_cov_5.237468, whole genome shotgun sequence.
ACCESSION   DAOTEI010000104 DAOTEI010000000
VERSION     DAOTEI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11905
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3772_length_11905_cov_5.237468"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="ftsZ"
                     /locus_tag="P5313_10650"
     CDS             complement(<1..615)
                     /gene="ftsZ"
                     /locus_tag="P5313_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRY80864.1"
                     /translation="MTVELVDDKSANPTVIKVIGAGGGGSNAVNRMIAAGVQNVQFIA
                     ANTDLQALSKSLAEVKLGLGAKVTHGLGAGGKPEIGEKAALEDREAIAQAVKGADMVF
                     VTAGMGGGTGTGSAPVIAQIARDSGALTVGVVTRPFDFEGRVKARIAEEGIEKLRQAV
                     DTLIVIPNQHLLKVVERRTPILDAFRFADDVLRQGVQGISDLITI"
     gene            complement(690..1916)
                     /gene="ftsA"
                     /locus_tag="P5313_10655"
     CDS             complement(690..1916)
                     /gene="ftsA"
                     /locus_tag="P5313_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRY80865.1"
                     /translation="MIVGLDIGTSKTCAVIGEYAESGLLEITGVGTAPSTGLRRGVVV
                     NIEATLRSVAQAVEAAELMSGREVRECWVGVAGANVEGINSRGVVAVTGKGREITQAD
                     VDRVLEAARAVVIPMDREILHVVPQTYIVDDQKGIRNPLDMIGVRLEAEVHIITGSVT
                     TAQNLVRCVNRAGFKAEALILQSLAAAKAVLSQDERELGCLLLDLGGGTTDALVYSEG
                     APYYTNVVPAGGIQVTNDLSIMLNIPLDAAEKIKLQSGCCYLPLVDPEETVIVPGVGG
                     RPPQEVSRQRIASIIQPRMEEIFGIARDRVEQMGYSRHVKGGVVLTGGGALMPGAAEL
                     ATSVFDLPARVGSPYTVGGLVEEYRSPLYSTAVGLVLAGAERTAPRTPEARPAADRKG
                     PGLAGRILDWVRKEFF"
     gene            complement(1921..2754)
                     /locus_tag="P5313_10660"
     CDS             complement(1921..2754)
                     /locus_tag="P5313_10660"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRY80866.1"
                     /translation="MSNAAAYDVRRAGTAASRKAPRGSDAGETGRRVLLGLIVGLVPL
                     AAVSLLLVFPAMKISRLEISGDTTLGREEVLQWAALHAVEYLATVDCPRIAANIQAHP
                     QVAEAKVSRLFPNGLRVTVTDRKAVAAVLATVDGRAVPVGVDRDGVAFRALPAGSEVP
                     ILSGIRFEGFRYGVRLPDTLLPVLRSLEALREAGPELLSAFSELRLVRRPYGELELLL
                     YPLQYRVPVRTPTVLNAELLRSIILVLDVVERQGLSPTISELDFRTGTVVYRVKEGIS
                     G"
     gene            complement(2747..3901)
                     /locus_tag="P5313_10665"
     CDS             complement(2747..3901)
                     /locus_tag="P5313_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRY80867.1"
                     /translation="MSAGFEVERTYTGRGGDTSLLAASALLFGTGLAVLYSASYGYAL
                     SLQMSAAHFLLRQLGYAPLALMVFLACAFLPAERLKSLVKPLTLLALAALVLPFIPGI
                     GVSRNGATRWIGIGSRTFQPSQIFRIALVLYLAHIFSKKSERIGDVVNSALPPLVITS
                     LGALLIFLQNDFSSAVILVLVAAALFWAAEVPVRFFAALGTILLPLTALSVLTSDFRL
                     RRIIGFLYPGYEPTALGYQVNGSLRAIRAGGFWGKGLGLGTLKLTSIPEVQSDFVFSA
                     FAEEAGFLGVLAFLGLWGFFLWRGYRTAFRNEDRFRSYLAFGLSTSLGIQVLVNIAVA
                     AGALPATGIALPFYSAGGSSFLEAAAACGLLYNLSRGAPGTRADTEVPHE"
     gene            complement(3911..4993)
                     /gene="mraY"
                     /locus_tag="P5313_10670"
     CDS             complement(3911..4993)
                     /gene="mraY"
                     /locus_tag="P5313_10670"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRY80868.1"
                     /translation="MFLEWIYPLEKYFSALNVFRYLTFRSAYAAVTALLIAFLFGPAI
                     IERLKVFKFGQSVRTDGPQTHLVKTGTPTMGGVFIILSVTAAVLLWVDLHNVYAWICL
                     LALLGCGAVGFLDDWLKIKRKNSDGISARLKLALQGLVAVAVVALLAVNRTEHTTMLY
                     LPFFKNPVVDLGLFYYPLAVVYIVGWSNAVNLADGLDGLATGLTIMALVAFSIITYIT
                     GRADWSSYLAIPFVRGAGELTVFNLALMGACVGFLWFNSHPAEVFMGDVGSLSLGGVL
                     GVLSLIVKKEILLFIIGGVFVLEEASVILQVGYFKLSKGKRLFKMAPLHHHFELGGWK
                     ETQVVIRFWILGGLFAIIALSTLKIQ"
     gene            complement(4997..6391)
                     /gene="murF"
                     /locus_tag="P5313_10675"
     CDS             complement(4997..6391)
                     /gene="murF"
                     /locus_tag="P5313_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRY80869.1"
                     /translation="MAEEASVLFRADWLSASAGGRLAGNAGAVVSAVEVDSRAVRPGA
                     LFAALPGERADGHDFIVPALAAGATLILASDEGWKRRSAVLDPALARAGAAAVLVPDS
                     LAALQEAARAWRLRFPSLTRVGITGSSGKTTTKECLGRILARKYRTAVNPGNLNSEIG
                     LPQAMFLLRPEHEAGVFEMGINHAGEMDALVRVWEPSHALVTNIGTAHIGILGSRRAI
                     AEEKRKIFSRISGAAFAWEDDEFLSFLAEGLPVPLRTFGPRTLEGLEGFRDAGLDGWI
                     LRYRGTEIAFPLPGTHNLLDALAAIALAAALGCGPGEIREGLEGVEALFGRSEIFRGE
                     VTVLRDCYNSNPDSAARALEFCDGLSWPGRRIYALGSMLELGAESGAEHRALGRRAGS
                     SRADALLFFGAETVEAARAAREAGFSGPVVQTEDFDELARAAAELAREGDLWLLKASR
                     GMELERLTDILRRG"
     gene            complement(6384..6674)
                     /locus_tag="P5313_10680"
     CDS             complement(6384..6674)
                     /locus_tag="P5313_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRY80870.1"
                     /translation="MKRVLALFAILTVPLLLLATVRQTARFQTLRAEALRLEALQKDW
                     IEQNRKLQAAVSVLSSRSRVDEAARQSLGLSPVRPEGILRIRIESPGSGIDG"
     gene            complement(6671..7708)
                     /gene="rsmH"
                     /locus_tag="P5313_10685"
     CDS             complement(6671..7708)
                     /gene="rsmH"
                     /locus_tag="P5313_10685"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRY80871.1"
                     /translation="MDIVHVPVLLAEVLDRLIPPRPDGLMVDATLGEGGHAEAFLSRY
                     PELRYLGVDADEVIQAKARKRLAPYGDRIRFARMYFDEFFGEFRKEVEEGRQERPGIV
                     LFDLGVSMFHFRESGRGFSLQGDEPLDMRLDSTTGRTASDLVNGLDRTELERIFRDFG
                     EEPFSGRIARVIVEERGGHPFSTSSRLAQTISRAVPGKFRHGRIHPATRCFQALRIAV
                     NDELGRAERALRDAAESLEVGGLLGVISFHSLEDRIVKNLFRSLARTPDPEASESDAA
                     LKGAPGAPIPKTRGPLLEILTRKPIAPGPEETAANPASRSAKLRIARRADPERPAPDK
                     YRGRREEEQSS"
     gene            complement(7717..8160)
                     /gene="mraZ"
                     /locus_tag="P5313_10690"
     CDS             complement(7717..8160)
                     /gene="mraZ"
                     /locus_tag="P5313_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRY80872.1"
                     /translation="MELLTGEFRNTLDEKGRLSLPAKLRGELPGNVLVVTQGIDSCLW
                     LFPPEQWRALSAKLLEQTSLFHARARLIQRRILAPSQEMEIDKAGRIAIPQALREFAG
                     LSKDCVILGITKYIEIWDAEAYRKYLEANESEFLAAAEELGGLTF"
     gene            8373..8627
                     /locus_tag="P5313_10695"
     CDS             8373..8627
                     /locus_tag="P5313_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80873.1"
                     /translation="MTVETNYVVFPEGDTQEIPGRLSIDQIVDHNGYPLPLPLSSARI
                     IAFRVVKIRHTETRGMLDILHYLELIPAYELMGYVGNVNR"
     gene            complement(8697..9374)
                     /locus_tag="P5313_10700"
     CDS             complement(8697..9374)
                     /locus_tag="P5313_10700"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HRY80874.1"
                     /translation="MEDIVRGAVDELFDSEERSPKLGFCTCAQCRLDVSCYVLNRIPP
                     EYVTSGRGVVHGETDWSGKVQRTADVLTLVREGWTRINQKKRPQYHQGSPAVSTELPS
                     GPVFNFPSVMGRLLNGVDFSPLADIQVGLYREGELLPMIDPNWQNPCRIVQETEGLYI
                     FWPRPLPARSPGEEAVFQLRVHSELPGFEEVSHYFELSLVAEPSAVDQITVRKVHKLP
                     DLYVFPK"
     gene            complement(9374..10219)
                     /locus_tag="P5313_10705"
     CDS             complement(9374..10219)
                     /locus_tag="P5313_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY80875.1"
                     /translation="MSWRPGAELLAALEGKPSPWADRFVRRAGAAVHRFGMIRDGDGV
                     LAGVSGGKDSLALCLALELRRRRVRDKYRLAALLVDWEDAPAPAGDLEDLGAYFELLG
                     VPFRVRSASRASPRTGAWGCYECAQARKRELFRAARESGFGTVALGHHLDDMAETLLL
                     NLVLHGRFEGMEPARSFFGGEVRIVRPLCDLREGSVRVFAERMELPVLASDCPRGVEN
                     PRRRAKDLLVEMGKIHRLVREHLFAAYAGASAAAEADRGKDPEGLRPGARKTDRGGRT
                     WKSTI"
     gene            10322..11026
                     /locus_tag="P5313_10710"
     CDS             10322..11026
                     /locus_tag="P5313_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80876.1"
                     /translation="MIPPRNAGLGLAVLAIALAACTRSPAGTPPPGPPGVPAVEPASA
                     APEPRSPERPSPPGVPGPRPVPAVPGTEALRAAAPDRLIGSRPLERLLPSHFTLGPLE
                     DLSGSDPGPRAEAAAAARKFLEGILAGRVDGAVPDARDVIEFMVGDLLEPDSRPLGWR
                     LGRGIPRPEGGWIFPILLSTGTGGIFGEVFAAETPGPGWGLDLVILNREPESAASGQS
                     PGAFDPTIRTVKPTGR"
     gene            11033..>11905
                     /locus_tag="P5313_10715"
     CDS             11033..>11905
                     /locus_tag="P5313_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80877.1"
                     /translation="MAVPVKRVEREFLLRAAGDENQEIFLSAGGAEWSVRIAEVRDKF
                     LVFRHSAPLHALVKGSILDFTFLVRGQTVAFRARVKEAGEARLVTEMPDTVWKNLSRR
                     YPRMTPPAGLSVSFSFKGERYELPFPSSRAFDPVEEPQADPEFEPRDIRKLMQEFYRK
                     AAKIASDRAVVMFKSRGPEGIEEELLTRHGRIYYLPTAIGGVPVVDPYASQRILTRDD
                     FLALFEERGVEPSYREDELARFERAKRVEGVLSELKIPVLFQEYVVGFVHILNRQPGK
                     APFDLGVLDTFAQSA"
BASE COUNT         1990 a         4127 c         3947 g         1841 t
ORIGIN      
        1 gatggtgatg aggtccgaga tcccctggac cccctggcgc aggacgtcgt ccgcgaagcg
       61 gaaggcgtcc aggatggggg tgcggcgctc cacgaccttc aggaggtgct ggttgggtat
      121 gacgatgagg gtgtccacgg cctggcggag cttctcgatc ccctcctccg cgatccgggc
      181 cttgaccctg ccctcgaaat cgaagggccg ggtgacgacc cccacggtga gggccccgga
      241 gtcccgggcg atctgggcga tgaccggggc ggagccggtg cccgtcccgc cgcccatgcc
      301 ggcggtcacg aagaccatgt ccgcgccctt cacggcctgg gcgatggcct cccggtcctc
      361 cagggccgcc ttttccccga tctcgggctt gcccccggcg cccaggccgt gggtgacctt
      421 ggcccccagg cccagcttca cctccgccag ggacttgctc agggcctgca ggtccgtgtt
      481 ggcggcgatg aactggacgt tctggacgcc cgccgcgatc atgcggttga ccgcgttgga
      541 gcctccgccc cccgctccta tgacctttat caccgtcgga ttcgccgact tgtcgtccac
      601 cagttctacc gtcatgctcc cctcccaggt tcacggcgtt cttcggttcg atgctcgttg
      661 cgttatgggc gcgttccgga ttcctcgttc tagaaaaact ccttgcggac ccagtccagg
      721 atgcggcccg ccagccccgg tcccttccgg tcggccgcag gccgggcttc cggagtccgg
      781 ggcgccgtgc gctcggctcc cgccaacacc aggccgacgg cggtggaata caggggactc
      841 cggtattcct caaccaggcc cccgacggtg tagggggagc cgacccgggc gggcaggtcg
      901 aacacggacg tggccagctc cgccgcaccg ggcatcaggg ctccgccccc ggtcagcacc
      961 accccgccct tcacgtggcg ggaatacccc atctgctcca cccggtcccg ggcgatcccg
     1021 aagatctcct ccatccgggg ctggatgatg gaggcgatcc gctgccggga tacctcctgg
     1081 ggaggccggc cgccgacacc gggcacgatg accgtctcct cgggatccac caggggcagg
     1141 tagcagcagc cggactggag cttgattttc tccgccgcgt ccagggggat gttcagcatg
     1201 atggacaggt cgttggtgac ctggatgccc cccgcgggca cgacgttcgt gtagtacgga
     1261 gcgccctcgc tgtagaccag ggcgtccgtg gtcccccccc ccaggtccag taggaggcag
     1321 ccgagctccc gctcgtcctg ggagagcacc gccttggcgg cggccaggct ctggagaatc
     1381 aaggcttcgg ccttgaagcc cgcgcggttc acgcagcgca ccaggttctg ggcggtcgtg
     1441 acggatccgg tgatgatgtg gacctccgcc tccaggcgga ctccgatcat gtccagggga
     1501 ttgcggatcc ccttctggtc gtccacgatg taggtctggg gaaccacgtg caggatctcc
     1561 cggtccatgg ggatgaccac ggcccgggcc gcttccagga cccggtccac gtccgcctgg
     1621 gtgatctccc ggcccttgcc ggtgacggcc acgacgcccc gggagttgat cccctccacg
     1681 ttggccccgg ccacccccac ccagcattcc cggacttccc gtcccgacat gagctccgcg
     1741 gcctccacgg cctgggccac ggaccggagg gtggcctcga tgttcacgac caccccccgg
     1801 cggaggcccg tggacggggc ggttccgact ccggtgatct ccaggaggcc gctttcggca
     1861 tattccccga taacggcgca ggtcttcgac gtgccgatat cgagtccgac gatcaggttc
     1921 tcagccagaa attccctcct tgacgcggta gaccaccgtg cccgtccgaa aatcgagctc
     1981 cgaaatggtc ggagaaagtc cctgcctctc cacgacgtcg agaacgagga taatagagcg
     2041 cagcaattcc gcgttcaaga ccgtgggtgt ccggaccgga acgcggtact gcaggggata
     2101 caggaggagt tccaactctc cgtagggcct ccggaccagg cgcagctccg aaaaggccga
     2161 gagcagctcc ggcccggcct cccgcagggc ctccagggac cgcaggacgg gaaggagggt
     2221 gtccggaagg cgcactccgt agcggaaccc ctcgaagcgg atccccgaga ggatcggaac
     2281 ctcgctgccc gccggcaggg cccgaaaggc gaccccgtcc cggtccacac ccaccgggac
     2341 ggcccggccg tccacggtcg ccaggaccgc cgccacggcc ttccggtccg taaccgtcac
     2401 ccgcagcccg ttcgggaaga ggcgggagac cttggcctcc gccacctggg gatgggcctg
     2461 gatgttggcc gcgatgcgcg ggcagtccac ggtggccagg tactcgaccg cgtggagggc
     2521 cgcccattgc aggacctcct cccgtcccag ggtcgtgtcc ccggagatct ccagccggga
     2581 gatcttcatg gcggggaaca ccaggaggag ggagaccgcg gccaggggca ccagcccgac
     2641 gatgaggccc aggaggaccc tccggcccgt ctcgccggca tccgagcccc ggggggcctt
     2701 ccgggacgcc gcggtcccgg cccgtcggac gtcgtacgcg gccgcgttac tcatggggca
     2761 cctccgtgtc cgcccgggtt cccggggctc cccgggaaag gttgtagaga agtccgcagg
     2821 ccgcggcggc ctccaggaag gacgagccgc ccgcggaata gaacggaagg gcgatcccgg
     2881 tggccggaag ggcgccggcg gcgacggcta tgttcaccag gacctggatt cccaggcttg
     2941 tggacagtcc gaaggccagg taggaccgga agcggtcctc gttccggaag gccgtccggt
     3001 aaccccgcca caggaagaag ccccagagcc ccaggaaggc caggactccc agaaaccccg
     3061 cttcctccgc gaaggcggag aacacgaagt ccgactggac ctcgggtatg ctggtcagct
     3121 tcagggttcc caggcccagc cccttgcccc agaagccccc cgcccggatg gcccggaggg
     3181 atccgttcac ctggtacccc agggcggtgg gttcgtaccc ggggtagagg aaaccgatga
     3241 tcctccggag ccggaagtcg ctggtcagta cggagagggc cgtcaagggg aggaggatcg
     3301 tccccagggc cgcgaagaag cggaccggga cttccgccgc ccagaacagg gccgcggcca
     3361 cgagcaccag gatcaccgcg gaggagaagt cgttctgcag gaagatgagc agggcgccca
     3421 gggaggtgat caccaggggc ggcagggcgg agttcaccac atccccgatc cgttcgctct
     3481 tcttggagaa gatatgggcc aggtagagga ccagggcgat ccggaagatc tgggagggct
     3541 ggaaggtccg ggaaccgatc ccgatccaac gggtggcccc gttccggctt accccgatgc
     3601 ccgggatgaa gggcagcacg agggcggcca gggccagtag ggtcaggggt ttcaccaggg
     3661 acttgagccg ttcggcgggc aggaaggcgc aggccaggaa caccatcagg gccaggggag
     3721 cgtaccccag ctggcgcaac aggaagtggg cggcggacat ctgcagggac agggcgtagc
     3781 cgtacgaggc ggagtagagc accgccaggc ccgtgccgaa gagcagggcc gaggccgcga
     3841 gcagggacgt atcccctccc cgacccgtgt aggtgcgttc gacctcgaaa cccgcggaca
     3901 tccccgcctc ctactgaatc ttcagggtgg agagggctat gatcgcgaag agccctccca
     3961 ggatccagaa ccgaatgacc acctgggtct ccttccaccc gcccagctcg aagtggtggt
     4021 ggaggggcgc catcttgaac aaccgctttc ccttgctcag cttgaagtac cccacctgca
     4081 ggatgaccga ggcctcctcc aggacgaaga cccctccgat gatgaacagg aggatctcct
     4141 tcttcacgat cagggacagg acccccagga cgccgcccag ggacagggag cccacgtccc
     4201 ccatgaagac ctccgcgggg tggctgttga accataggaa gcccacgcag gctcccatca
     4261 gggccaggtt gaagaccgtg agctccccgg ctccccggac gaagggtatg gccaagtagc
     4321 tcgaccagtc cgcccggccg gtgatgtagg tgatgatgga gaaggccacc agggccatga
     4381 tggtcaggcc cgtggccagg ccgtccaggc cgtccgccag gttcaccgcg ttggaccagc
     4441 ccacgatgta gaccaccgcc aggggatagt agaacagccc gaggtccact acggggttct
     4501 tgaagaacgg gaggtacagc atcgtggtgt gttccgtccg gttcaccgcc aggagggcca
     4561 ccacggcgac cgctaccaac ccttgcaggg caagcttgag ccgggcggag atcccgtccg
     4621 agttcttccg tttgatcttg agccagtcgt ccaggaagcc gacggccccg cagcccagga
     4681 gggccagcag gcagatccag gcgtacacgt tgtggaggtc gacccagagc aggaccgcgg
     4741 cggtcacgga caggatgatg aagacccctc ccatggtcgg ggttccggtc ttgaccaggt
     4801 gggtctgggg gccatcggtc cggacggact gcccgaactt gaagaccttc aggcgttcga
     4861 tgatggcggg accgaagagg aaggcgatca gcagggcggt caccgccgcg taggcggacc
     4921 ggaaggtaag gtaccggaac acgttcaacg cggagaagta tttttccagc ggatagatcc
     4981 attccaggaa cacggcttac ccccgtctca gtatgtcggt caggcgctcg agctccatgc
     5041 cccgggaggc cttgaggagc caaagatcgc cttcccgggc gagttcggcg gccgcccggg
     5101 ccagttcgtc gaaatcctcc gtctggacca cggggcctga aaatccggcc tcccgcgcgg
     5161 cccgggcggc ttcgacggtc tccgcgccga agaacagcag ggcatcggcc cgggaggagc
     5221 ccgcccgacg gcccagggcc cggtgctcgg ccccggattc cgctccgagt tccagcatcg
     5281 atcccagggc gtagattctc cgtccgggcc aggaaagacc gtcgcagaac tccagggccc
     5341 gggcggcgga atccgggttg gaattgtagc agtcccgcag tacggtcacc tctccccgga
     5401 agatctcgga cctcccgaag agggcctcga ccccctcgag cccctcccgg atctccccgg
     5461 ggccgcagcc cagggcggcc gccagggcga tcgccgccag ggcgtccagg aggttgtggg
     5521 tccccggcag ggggaaggcg atctccgttc cccggtagcg aaggatccag ccgtccaggc
     5581 ccgcgtcccg gaatccctcc agaccctcca gggtgcgggg gccgaaggtc cggagcggga
     5641 cgggaagccc ctccgccagg aaggaaagga actcgtcgtc ctcccaggcg aaggccgcgc
     5701 cggagatgcg ggaaaaaatc ttccgttttt cctcggcgat ggcccgccgg ctgccgagaa
     5761 ttccgatgtg ggcagtcccg atgttcgtga ccagggcgtg cgaaggctcc cacacccgga
     5821 ccagggcgtc catctccccg gcgtggttga tccccatctc gaagaccccc gcctcgtgct
     5881 ccggccgaag gaggaacatc gcctggggca ggccgatctc ggagttgagg ttcccgggat
     5941 tcaccgcggt ccggtacttc cgggccagga tgcggcctag gcactccttg gtcgtggtct
     6001 tgccgctgga tccggtgatc cccacgcgcg tcagggacgg aaatcgcaga cgccaggccc
     6061 gggccgcctc ctgcagggcc gcgaggctgt ccggaaccag gacggccgcc gccccggcgc
     6121 gggccagggc ggggtccagg acggcggaac ggcgcttcca cccttcatcc gaagccagga
     6181 tcagggtcgc gcctgcggcc agggccggaa cgatgaagtc gtggccgtcc gcccgctccc
     6241 cgggcagggc cgcgaagagg gccccggggc ggacggcccg ggaatccacc tccaccgcgg
     6301 agacgacggc gccggcattc ccggcaagcc gcccgcccgc ggaggcggaa agccagtccg
     6361 cccggaacag gacggacgct tcctcagcca tcgatgccgc tcccggggct ttcgatccgg
     6421 atgcgcagga tgccctccgg ccggaccggc gacaggccca ggctctgccg ggcggcctcg
     6481 tctacccggg accgggagga caggaccgag acggcggcct ggagcttgcg gttctgctcg
     6541 atccagtcct tctggagggc ttccaaccta agggcctccg cgcggagggt ctggaaccgg
     6601 gcggtctgtc gcaccgtggc cagcaggaga aggggaaccg taaggatggc gaataaggca
     6661 aggacgcgct tcacgatgat tgctcttcct ctctccggcc gcggtatttg tccggagccg
     6721 gtcgttctgg atccgccctt cgggcgatcc gcagtttcgc gctccgggag gcggggttgg
     6781 ccgccgtctc ctccggtccg ggcgcgatag gctttcgggt caggatctcg aggaggggac
     6841 ccctagtctt aggtatcggc gcgcccggcg cgcccttgag ggccgcgtcg gattccgacg
     6901 cttcgggatc cggggtccgg gccagggatc ggaacaggtt cttcacgatc ctgtcctcca
     6961 gcgaatggaa gctgatgacc cccaggagcc ccccgacctc cagcgattcc gccgcgtccc
     7021 ggagggcccg ttccgcccgg cccagctcgt cgttcaccgc tatccggagg gcctggaagc
     7081 accgggtcgc cggatggatg cggccgtggc ggaactttcc cgggactgcc cgggaaatcg
     7141 tctgtgcgag gcggctggac gtcgaaaagg gatgcccgcc ccgctcctcc acgattaccc
     7201 gggctatgcg cccggaaaac ggctcttccc cgaagtcccg gaagatcctt tccagctccg
     7261 tgcggtccag cccgttcacg agatccgagg cggtcctccc agtcgtgctg tccagcctca
     7321 tgtccagcgg ctcatcgccc tggaggctga acccccgccc cgattcccgg aaatggaaca
     7381 tggacacgcc caggtcgaac aggacgatcc cgggacgctc ctgtctcccc tcttccacct
     7441 ccttccggaa ctctccgaaa aactcgtcga agtacatgcg cgcgaaccgg atcctgtcgc
     7501 cgtacggcgc aagccgtttc cgcgccttcg cctgaatcac ctcgtccgcg tccaccccca
     7561 ggtaccgcag ttcggggtac cgggaaagga aggcctcggc gtgccctccc tcccccaggg
     7621 tcgcgtccac catgagcccg tccggtcggg gcgggatcag gcggtccagc acctccgcaa
     7681 ggaggaccgg gacgtggacg atgtccaccc ctgcacctag aaggtcagcc cgccgagctc
     7741 ctcggcggcc gccaggaact ccgactcgtt ggcttccagg tacttcctgt aggcctcggc
     7801 gtcccagatc tcgatgtact tcgtgatccc caggatcacg cagtccttgg aaagccccgc
     7861 gaactcccgc agggcctggg ggatcgcgat gcgccccgcc ttgtcgatct ccatttcctg
     7921 gctcggggcc aggatccggc gctggatcag gcgggcgcgg gcatggaaga ggctggtctg
     7981 ctcgagcagc ttggcggaga gggccctcca ctgctcgggc ggaaagagcc agaggcagga
     8041 atcgatcccc tgggtgacga ccagaacgtt ccccggcagc tccccacgga gcttcgcggg
     8101 cagggagagc ctgccctttt cgtccagggt gttccggaac tcaccggtga ggagttccat
     8161 accactcttt cctattattt cccactattt cccacctgat cctatactat ccctcaaaat
     8221 tccattgtca accgtcaaga gtagacggaa tcgaggagac agaaaaaata atcataattt
     8281 atgacaacgg atgtatagtg gtttgaatct ggaccctaga cccgggaaat gtccccgttg
     8341 cgccgccctt gcggcgccga tagactgtgc ccatgaccgt cgaaacgaac tatgtggtgt
     8401 tcccggaggg ggacacccag gagatccccg ggaggctgtc catcgaccag atcgtggacc
     8461 acaacgggta tccccttccc cttcccctgt cctcggcccg gatcatcgcc ttccgggtcg
     8521 tgaagatccg gcatacggag acccggggaa tgctggacat cctgcactac ctggaactga
     8581 tccccgcgta cgagttgatg gggtatgtgg ggaacgtgaa taggtgaata agtgaatggg
     8641 tgaatagggc ggaggatctc tcccgattca cctattctcc ccttcaccca ttcacttcat
     8701 ttcggaaaca catacaaatc cggcagtttg tgcaccttgc gcacggtgat ctggtccacc
     8761 gcggagggct cggcgaccag ggacagctcg aaatagtggg agacctcctc gaaaccgggc
     8821 agctccgaat ggacccggag ctggaacacc gcctcctccc cggggctgcg ggccggcagg
     8881 gggcggggcc agaagatgta caggccctcg gtctcctgga cgatccggca gggattctgc
     8941 cagttggggt cgatcatggg gagcagctcg ccctcccggt agagccccac ctggatgtcc
     9001 gcgaggggtg agaagtccac gccgttgagc aggcggccca tcacggaggg gaagttgaac
     9061 accgggccgg aagggagttc cgtggacacc gcgggggagc cctggtggta ctggggccgt
     9121 ttcttctggt tgatccgggt ccagccctcc cggaccaggg tgaggacgtc cgcggtacgt
     9181 tggaccttgc cggaccagtc ggtctccccg tgcacgaccc cgcggccgga ggtgacgtac
     9241 tccgggggga tccggttgag cacgtagcag gacacgtcca gccggcactg ggcgcaggtg
     9301 cagaagccca gcttcgggga tcgctcctcc gaatcgaaga gctcgtccac ggcaccccgc
     9361 acaatgtctt ccattagatt gtggatttcc atgtccgccc tcccctgtcc gtcttcctcg
     9421 ccccgggccg caggccttcc gggtccttcc cgcgatccgc ttccgcggcc gctgaagccc
     9481 ccgcgtacgc ggcgaaaagg tgctcccgta ctagtctatg gatttttccc atctccacaa
     9541 ggaggtcctt ggctcgccgc cggggatttt caacgccccg gggacaatcc gaagccagca
     9601 cggggagttc catgcgctcc gcgaaaaccc gcacgctccc ctcccgcagg tcgcacaggg
     9661 ggcggacgat ccggacctcg cccccgaaga aggaccgggc cggctccatc ccctcgaagc
     9721 ggccgtgaag gaccaggttc aatagcaggg tttccgccat gtcgtccaga tggtggccca
     9781 gggccaccgt tccgaacccc gactcccggg cggcccggaa gagctcccgt ttccgggctt
     9841 gggcgcactc gtagcagccc caggccccgg tccgcgggga tgcccgggac gcggagcgca
     9901 cccggaaggg gacccccagg agttcgaagt acgccccgag gtcctccaga tccccggccg
     9961 gcgccggggc gtcctcccaa tccaccagga gggccgccag ccggtatttg tcgcggactc
    10021 gccgcctgcg gagctccagg gccaggcaga gggccaggga atccttgccg ccggaaaccc
    10081 ccgccaggac tccgtccccg tcccggatca tcccgaaccg gtggaccgcg gctcccgccc
    10141 tacggacgaa gcgatccgcc cagggcgacg gcttgccctc cagggcggcc aggagctccg
    10201 ccccgggacg ccagctcatc ggtcttcccc cgggcccgag aacactccgc gatcgatccg
    10261 gaagccgaat ccgcccgatt ccattgaccc cgcccgcccc ctgcctccat actacggtat
    10321 cgtgattcct ccgcgcaacg ccggcctcgg cctggccgtg ctcgcgatcg ccctggccgc
    10381 ctgcacccgt tcccccgcgg gcacccctcc acccggacct cccggggttc ccgcggtgga
    10441 gccagcctcg gccgccccgg agccccggag cccggaacgt ccctccccgc ccggggttcc
    10501 cggtccccgc ccggtccccg cggtccccgg gacggaagcc ctgagggcgg ccgccccgga
    10561 ccgcctgatc ggatcccgcc ccctggaacg cctccttccg tcgcacttca ccctgggccc
    10621 cctggaagac ctgtccggct ccgatccggg accgcgggcc gaagccgccg cggcggcacg
    10681 gaaattcctg gagggcatcc tggcaggacg ggtggacgga gccgttccgg atgcccggga
    10741 tgtgatagaa ttcatggtcg gggacctgct ggaaccggat tcccggcccc tgggctggag
    10801 gctcgggcgc gggattccgc ggcccgaagg cggctggatc ttcccgatcc tcctttcgac
    10861 agggacggga ggaatcttcg gtgaggtgtt cgccgcggaa acgccggggc cgggctgggg
    10921 cctggacctg gtaatcctga atcgggaacc ggaatccgcg gcgtccggac agagtccggg
    10981 cgccttcgat ccgacgatac ggaccgtgaa acccacggga aggtgaggca gcatggcggt
    11041 gccggtcaag cgcgtcgaac gggaattcct gctccgggcc gcgggggacg aaaaccagga
    11101 aatcttcctg tccgcgggag gggccgagtg gagcgtccgg atcgcggagg tgcgggataa
    11161 attcctcgtc ttccggcact ccgcgcccct ccacgccctg gtcaagggct ccatcctgga
    11221 tttcaccttc ctggtccggg gccagaccgt cgctttccgg gcccgcgtca aggaggccgg
    11281 ggaggcccgg ctggtcacgg agatgcccga cacggtctgg aagaacctga gccggcgcta
    11341 tccccgcatg acccctcccg cggggctctc ggtctccttc tccttcaagg gcgagcggta
    11401 cgagctcccc ttcccctcct cccgggcctt cgaccccgtg gaggagcccc aggcggaccc
    11461 ggagttcgag ccccgggaca tccggaagct catgcaggag ttctaccgca aggccgccaa
    11521 gatcgcttcc gaccgggccg tggtcatgtt caagtcccgg ggacccgagg ggatcgagga
    11581 ggagctcctg acccggcacg gccgcatcta ctacctgccc accgcgatcg gaggggtccc
    11641 cgtggtggat ccctacgctt cccagcggat cctgacccgg gacgacttcc tggccctctt
    11701 cgaggaacgg ggcgtggagc cgagctaccg ggaggacgag ctggcccggt tcgagcgcgc
    11761 caaacgggtg gagggggtgc tttcggagct gaagatcccc gtcctcttcc aggaatacgt
    11821 ggtgggcttc gtgcacatcc tgaaccgcca gcccggcaag gcccccttcg acctgggggt
    11881 cttggacacc ttcgcccagt ccgcc
//
LOCUS       DAOTEI010000105        11899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3775_length_11899_cov_4.813830, whole genome shotgun sequence.
ACCESSION   DAOTEI010000105 DAOTEI010000000
VERSION     DAOTEI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11899
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_11899_cov_4.813830"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="xseA"
                     /locus_tag="P5313_10720"
     CDS             <1..1039
                     /gene="xseA"
                     /locus_tag="P5313_10720"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRY80878.1"
                     /translation="QAVLFRGRASRLDFAPADGMLVRAEGSLSVYAARGQYQLVCENL
                     VRAGQGDILALLEERRRRLAAEGLFDPDRKRPLPRFPETVAVVTSPTGAAIRDILNVL
                     GRRNAGIRVIVLPAAVQGADAPVEIARQIRTANRYRMAEVLIVGRGGGSLEDLLAFSD
                     EEVVRAVAESEIPVISAVGHEIDWALTDFAADLRAPTPSAAAELVSESRGRLAETAAS
                     LGSSMAEEVRRRLERTRAVINQFDPESLELRFRRVLQPRLQRFDDLKEDLVLGMRDSL
                     VRSRHRFDLASLSLASASPQAVLSRGFAVVRSADTGRAVRDSRELSPRDRVTLQFARG
                     ASAAVIEESEP"
     gene            1036..1275
                     /gene="xseB"
                     /locus_tag="P5313_10725"
     CDS             1036..1275
                     /gene="xseB"
                     /locus_tag="P5313_10725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRY80879.1"
                     /translation="MKQFEERLRRLEELSEKIRDPELPLEEAVAVFEEGIKLSKGLER
                     DLAKIESRVEILLNQPAAPEDKPELGLFDQPGADS"
     gene            1730..2092
                     /locus_tag="P5313_10730"
     CDS             1730..2092
                     /locus_tag="P5313_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80880.1"
                     /translation="MGGTLATQLFLMRLKPLLAARKLAWKPRNRRKTKEFMLDEGLSE
                     EDAYEIIADLRPEHYAKGPEPDDDGSPGDVMIFYQPYTRQAPPGDGILLYIKLKIWTD
                     TDGDAGIVMSFHDEGNYE"
     gene            2070..2792
                     /locus_tag="P5313_10735"
     CDS             2070..2792
                     /locus_tag="P5313_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80881.1"
                     /translation="MMRAIMSNRQPCSRCGTTRDIEIVERVEQVTIKGKEVSFNAHYS
                     RCLTCGDEFEAPGQLDENLEAAREAYALLYEAPSPADLVALRTRYGASQKAFGAILGF
                     GELTMNSYEKGAPPGPANRLLLKLAADPCTFKAMYDINSGRIGAIQRRRIEESEGVRS
                     ALRWSGLGALSGCLTPIQREKIEACAERNDRTIPEQVAIYVGAASFQDYSRLYIEARW
                     SDETTTELPKTVVQPNHSQGAA"
     gene            2792..3232
                     /locus_tag="P5313_10740"
     CDS             2792..3232
                     /locus_tag="P5313_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80882.1"
                     /translation="MSGGINDRLTRFLHAITLQDIKPMLLRSEALGPMPAPGSNLTIE
                     CKQSFAQDDPLAPIPDTRVFRMKYELTVSFQDAPFFKQESGFIIAFGVTDLPTFTELW
                     ADEEVRKVFMEKQIQRTLWPLFRQHAHDGMSRLGMAPVSLPWLL"
     gene            3288..6350
                     /locus_tag="P5313_10745"
     CDS             3288..6350
                     /locus_tag="P5313_10745"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRY80883.1"
                     /translation="MKPASDATYPQGSDSKPLYVREQDIETAFIEKLRGLKYAYRPDI
                     RDRAALELNFRGKFEALNRVHLTDGEFQRLLDELVTPDVFAASKVLREKSDFFRDDGT
                     PLSYTLVNIRDWCKNDFEVVHQLRINTDYSHHRYDVLLLINGVPVVQVELKTLGVSPR
                     RAMEQVVEYKSDPGNGYTRTLLCFIQLFIVSNRTETFYFANNNPRHFAFNASEQFLPV
                     YRFADPDNTPVNHLDDFSDRFLQKCVLGRMISRYMVLIQSEQKLLIMRPYQIYAVQAI
                     LDCIEQDNGNGYIWHTTGSGKTLTSFKASTLLKDNPAIDKCLFVVDRKDLDRQTREEF
                     NRFQEGCVEENTNTAALVRRMLSEDKADKVIVTTIQKLGLALDENSKRNRPRADRGRE
                     TFTERLEPLRNKRFVFIFDECHRSQFGDNHQAIRDFFPRAQLFGFTGTPIFEENATRV
                     QVEDDRASFRTTVDTFQRELHRYTITHAIEDRNVLRFHVDYYRPEGEAELKPGETLKK
                     EKIVAAILDKHDAATHQRRFNALLATASIDDAVEYFGLFRTLQAERRVEDPDFVPLKI
                     ACVFSPPADGDADVRQLQEDLPQEKADNEKDPDRKKAALRSILEAYNSSYGTNHSLGD
                     FDLYYQDVQKRIKDQKFPNADLPRRGREKIDLTIVVDMLLTGFDSQYLNTLYVDKNLR
                     HHGLIQAFSRTNRVLNAAKPHGQILDFRQQQAAVDAAIVLFSGVESDTKAREIWLVEP
                     APKVVEKLTAALSRLDDFLRSQGLKPEPESVPALRGDEARAAFVERFREVQRLKTQLD
                     QYTDIPPEAEAAVERLLPRDRLNAFRGVYLETAQRLRAERDRDGAAGESAADQLDFEF
                     VLFASADIDYDYIMALLARFSNESPDKLKMSRDQLIGLIQADAKFLDEGDTIADYVRT
                     LRAGQGLDQKEIEAGYRRFRDERRAAELAGLARKHGLAPDRLRTFTDAILERRVFDGE
                     RLTELLEPRSLPWKDRRAAELALMEDLVPLLKKRVGGRDISGLSAYES"
     gene            6368..7693
                     /locus_tag="P5313_10750"
     CDS             6368..7693
                     /locus_tag="P5313_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRY80884.1"
                     /translation="MNDDKPRLTPKLRFPEFRDAEGWTGMRMSEVYSFKGNNSFSRDQ
                     LNYNQGIVKNIHYGDIHTKFSTLFDVTCETVPFINPTESLDGFRPDNDCVEGDIVFAD
                     ASEDMDDIGKSIEIVRLNGERVVAGQHTILARPNDDILVIGFGAYLFKSKHIRTQIQK
                     ESQGAKVLGLSASRLTKIILPIPRSKAEQHKIAACLSSLDDLIDAETRKLDALKAHKK
                     GLLRNLFPREGETRPWLRFPEFRDAGEWKYGKLTELCETIMDGTHFSPKTKIGPRPYL
                     TSKNIHNGKIDLSNVSYISEEEHNAIYNRCPVQWHDVLLTKDGANTGNCALNILDFEF
                     SLLSSVAVLRGKRDSLDQEFLFHSVLSDYLQTQIIDSMSGQAITRVTLEKIGNFSIAV
                     PSIPEQHRIASCLSSLDDLISARTDRIEAFKTHKKGLMQQLFPFSEVNL"
     gene            7690..8769
                     /locus_tag="P5313_10755"
     CDS             7690..8769
                     /locus_tag="P5313_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY80885.1"
                     /translation="MTLPEIATLLKQSDAKIILVFGFNAVGKTQLSVAYKNETKLTDG
                     THTGVYYNAYSEDLFVWNNDEENNGADTRLIVQPSTLSKFHSSLTEGIIRKKLKAYKP
                     KYDFEFTMHDDVEKGIKSISFFIPNSDLDVATPSIKISRGEERIFVWCFFLALFEVEG
                     WADINSSHLFIDDPVSSLDDHNIFITSSTLFDLIDEYYEKRKIIIATHHIGLFSILFD
                     YLQKGEKSDKYKRYTCPMILSSKHGDLTLETCKKDVFLYHLRLLQILKQAQDMDEVKS
                     YHFVLLRQVLENMASFLGTGKVGHVLQVIGVEDSDTYSQTVNTLSHKNIYLYESDELV
                     EDNKRLFSDILGKLMDQFKFVLHAE"
     gene            8773..10386
                     /locus_tag="P5313_10760"
     CDS             8773..10386
                     /locus_tag="P5313_10760"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HRY80886.1"
                     /translation="MTDYNHQQLGKTLWAIADQLRGAMNADDFRDYMLSFLFLRYLSD
                     NYEAAAKKELARDYPAIPASDPRVPLAVWYANNPDDIVDFEKQMRRKIHYVIKPDHLW
                     TNIANLARDQSPDLLDTLRDGFKYIENESFESTFQGLFSEINLGSEKLGRTHADKNKK
                     LCDIVKEIAKGLSEFSTDVDSLGDAYEYLIGQFAAGSGKKAGEFYTPQQISTILSRIV
                     TLDGQEPATGKVPRLGSVLDFACGSGSLLLNVRKQMGPHGIGRICGQEKNITTYNLAR
                     MNMLLHGVKDTEFEIYHGDTLTNDWDFLRETNPARMPKFDAVVANPPFSYRWEPKEDM
                     ADDVRFKNHGLAPKSAADFAFLLHGFHYLSDQGVMAIILPHGVLFRRGAEEKIRTKLL
                     KDGHIDTVIGLPANLFYSTGIPVCVLVLKKCKKPDDILFINAAGHFEKGKRQNVLLDE
                     HIEKIVDTYQHRREEARYSARVPMGRIAEEGYNLNISRYISTAQEEPEIDLASVHSDL
                     VRIEDEIKTAQDKHNEFLKELGLPLLPPG"
     gene            10448..11527
                     /locus_tag="P5313_10765"
     CDS             10448..11527
                     /locus_tag="P5313_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80887.1"
                     /translation="MDQKLLDHCIQEYKERSITNPEEYRQARQERSERVAWFQAWNRA
                     RLLTLNADSLEEYLSRLWAMLIWGNKRYYTDLIIEKNTLEVVRDNLASLLYDDAPVAI
                     RWDRFRDCVSQIGPAMVSELLAHYYPSRFPVWNRRAKEALERLGETSLPRYDYQIIGA
                     FYDRLIHLASSVADALSNAGFEDVDMLFVDYFFWDLVQEKNPPVIPSAVQSQTQGAQN
                     QQDQVFVHNEVRDKIAEIGTWLGFKTSTEQTVTTGTRIDVLWEASISNMGRVLYAFEV
                     QTSGSIDSLQLNLLRSLSNPAVQAVVAVSDERQLEKIKAEAATLPELKNKLRCWDSRE
                     VLETHAALSTAFDSINRLKLVPDAF"
BASE COUNT         2657 a         3773 c         3204 g         2265 t
ORIGIN      
        1 ccaggccgtc ctcttccggg gccgcgcctc ccgcctggac ttcgcccccg cggacgggat
       61 gctggtccgc gccgagggct ccctgtccgt gtacgccgcc cggggccaat accagctggt
      121 ctgcgaaaac ctcgtccggg ccggccaggg ggacatcctg gccctcctgg aggagcgcag
      181 gcgccgactg gccgcggagg gactcttcga cccggaccgg aagcgccccc tcccccgctt
      241 cccagagacc gtggccgtcg tgaccagccc caccggggcc gcaatccggg acatcctgaa
      301 cgtcctgggc cggcggaacg cggggatccg ggtcatcgtc ctgcccgcgg cggtccaggg
      361 cgccgacgcc cccgtcgaga tagcccgcca gatccgcacc gccaaccggt accgcatggc
      421 ggaggtcctg atcgtcgggc gcgggggagg ttccctggag gacctcctgg ccttttcgga
      481 cgaggaggtc gtacgggccg tggcggaatc cgagatcccc gttatcagcg ccgtggggca
      541 cgagatcgac tgggccctca cggacttcgc cgcggacctc cgcgccccca ccccctccgc
      601 ggccgcggag ctcgtgtccg agagccgggg ccgcctggcc gagacggccg cctccctggg
      661 ctcgtccatg gccgaggagg tccgccgacg cctggagcgc acccgggcgg tgatcaacca
      721 attcgacccc gagtccctgg agctccggtt ccgccgcgtc ctccagcccc ggctccagcg
      781 cttcgacgac ctcaaggagg acctggtcct gggcatgcgg gactccctgg tccgctcgcg
      841 ccaccgcttc gaccttgcca gcctctccct cgcctcggcc agcccccagg ccgtcctttc
      901 gcgcggcttc gccgtggtcc gaagtgcgga cacggggcgg gccgtccggg actcccggga
      961 gctatcccct cgggaccggg tcacattgca gttcgcccgc ggcgcctccg cggccgtcat
     1021 cgaggagtcc gagccatgaa acagttcgag gaacgactgc gccgtctgga ggagctttcc
     1081 gaaaagatcc gggatcccga gctgcccctg gaggaggccg tggcggtctt cgaggagggc
     1141 atcaagctct ccaagggcct ggagcgggac ctggccaaga tcgagagccg ggtggagatc
     1201 ctcctcaacc aacccgcggc cccggaggac aagcccgaac tgggcctctt cgaccagccg
     1261 ggggcggaca gctgaacctt tcggggtagc acccgcggcg gagctcctcg ggaagcgtcg
     1321 aatatccttc aagcaacgcg gggcctgctg cgggaagccc ggttccgcgt gttcgccgcc
     1381 ttcgcccaaa gggcgaaggc gggggttgca gacgtggctc ccccttgcta cgtcctcctc
     1441 gcgccctgaa gggcgctgca gtggactccg ctaccccctc gtaggttgga gccatatttc
     1501 aatcaaagac ggtatgattg accaccatat tgttaacacc ggggattgat atcttgtctt
     1561 ttccccggag ttcgctatca tgttttcatc tcaagaaaag gtaaaaccat gaaagcaagg
     1621 gtgccgttct atctatgtat actcattact tctattcttt ctaggaatgg attcttcaaa
     1681 tgagcccaca taccggtatt gcgcgatacc gataatggga gtatattgaa tgggcggaac
     1741 gctcgcgacc cagctattcc tcatgcgcct caagccactt ctagcggctc gaaagctcgc
     1801 ctggaagccc cgcaacaggc gtaagacaaa ggaattcatg ctggatgaag gacttagcga
     1861 ggaggacgcc tacgagatca tcgcagatct gaggcccgag cactatgcca aagggccgga
     1921 gcccgatgat gatgggtcgc ctggagacgt gatgatcttt tatcagccgt atacgcgaca
     1981 ggcaccaccg ggagacggga ttctgttgta catcaagctg aagatctgga ccgacaccga
     2041 tggggatgcc ggtatcgtga tgtcgtttca tgatgagggc aattatgagt aatcgacagc
     2101 cgtgctctcg ctgcgggaca acccgtgata tcgagatcgt cgagcgggtt gaacaggtga
     2161 cgatcaaggg caaggaggtt tctttcaacg cccactactc gcgctgtctc acttgcggag
     2221 acgagtttga ggcccccggc cagctcgacg agaatctcga ggccgcccgc gaggcgtatg
     2281 ccctgttgta cgaggctcct tctcccgcgg acctcgtggc tcttcgaacc cgctacggcg
     2341 ccagccaaaa ggccttcgga gccatcctcg ggttcgggga gctcaccatg aacagctacg
     2401 agaagggggc tccgcccggc cccgccaatc gtctacttct gaaactcgcc gccgacccct
     2461 gtacgttcaa ggcgatgtac gacatcaact ccggcaggat cggagccatc cagcgtcgcc
     2521 gaatcgagga gtccgaaggc gtccgatcgg cgctccgctg gtcggggctg ggcgcccttt
     2581 cgggatgtct cactccgatc caacgcgaga agatcgaggc ctgcgccgag cgcaacgacc
     2641 gtacgatacc ggagcaagtt gccatatacg tcggcgcggc ctcctttcag gactattctc
     2701 ggttgtatat cgaggctcga tggtccgacg aaacgaccac ggagcttccg aagacagtcg
     2761 ttcaacccaa ccacagccaa ggagcggcgt gatgagcggc ggcatcaacg acaggctgac
     2821 gagattcctc cacgcaatca ctctacagga tatcaaacct atgctgttgc gctccgaggc
     2881 gctcggccct atgccggctc cgggaagcaa ccttacgatc gaatgcaagc agtcctttgc
     2941 ccaggacgac ccgttggctc caataccgga tacaagggtg tttcgcatga aatacgagct
     3001 tacggtaagc ttccaggatg ctccgttctt caaacaggaa tccgggttca tcatcgcatt
     3061 cggcgtcacc gatctcccga cgttcacgga gttgtgggcc gacgaagagg tgcgaaaggt
     3121 tttcatggaa aaacagatcc aaaggaccct ctggcccctc ttccgtcagc acgcgcacga
     3181 cgggatgagc cgcctgggta tggcgccggt ttcacttccc tggcttctgt aggattggag
     3241 cgaggctgtc tatgtcggat cccgaatcga ataaaaacca cgattccatg aaaccagctt
     3301 ctgacgcgac ctacccgcag ggatccgatt ccaagcccct ctacgtccgg gagcaggaca
     3361 tcgaaactgc gttcatcgag aagctgcgcg ggctcaagta tgcctaccgt ccggacatcc
     3421 gcgaccgggc cgccctggag ctcaacttcc gcgggaagtt cgaggccttg aaccgcgtcc
     3481 acctcacgga cggcgagttc cagcgcctcc tggacgaact ggtcaccccg gacgtcttcg
     3541 ccgcaagcaa agtcctccgg gaaaagagcg acttcttccg ggacgacggc accccgctgt
     3601 cctacactct cgtcaacatc cgcgactggt gcaagaacga cttcgaggtc gtccaccagc
     3661 tccgcatcaa cacggactac agccaccacc ggtacgacgt cctgctactc atcaacgggg
     3721 tgcccgtggt ccaagtcgag ctcaagaccc tgggcgtcag cccccgccgc gccatggagc
     3781 aggtggtcga atacaagagc gatcccggca acgggtacac ccgcaccctc ctgtgtttca
     3841 tccagctctt catcgtcagc aaccgaaccg agaccttcta cttcgccaac aacaaccccc
     3901 ggcacttcgc cttcaacgcc tccgaacagt ttctcccggt ctatcgcttc gccgatccgg
     3961 acaacacgcc cgtaaaccac ctggacgatt tctccgaccg cttcctccag aagtgcgtcc
     4021 tgggccgcat gatcagccgc tacatggtcc tcatccagag cgagcagaag ctactcataa
     4081 tgcggcccta ccagatctac gcggtccagg ccatcctgga ctgcatcgag caggacaacg
     4141 gcaacggcta catctggcac accaccggca gcggcaagac cctcacctcc ttcaaggcat
     4201 ccaccctcct caaggacaat ccggccatcg acaagtgcct cttcgtcgtg gaccgcaagg
     4261 acctggaccg ccagacccgg gaagagttca accgcttcca ggaaggctgc gtggaggaga
     4321 acaccaacac cgccgccctg gtccggcgca tgctctccga ggacaaggcg gacaaggtca
     4381 tcgtcaccac catccagaag ctcggcctgg ccctggacga gaacagcaag cgcaaccgcc
     4441 cgagggccga ccgcggccgc gagacattca cggagcgcct ggagcccctg cggaataagc
     4501 gattcgtctt catcttcgac gagtgccacc ggtcccagtt cggggacaac caccaggcca
     4561 tccgggactt ctttccccgg gcccagctct tcggattcac cggcacgccc atcttcgagg
     4621 agaacgccac ccgcgtccag gtcgaggacg accgggcgtc cttccgcacc accgtggaca
     4681 ccttccagcg ggaactgcac aggtacacca tcacccacgc catcgaggac cggaacgtcc
     4741 tgcgcttcca cgtggactac taccgccccg agggcgaggc ggagctcaag ccgggtgaga
     4801 cgttgaagaa ggagaagatc gtcgcggcca ttctggacaa gcacgacgcc gccacccatc
     4861 agcgccgctt caacgccctc ctggccacgg cctccatcga cgacgccgtc gaatacttcg
     4921 ggctcttccg aaccctccag gccgagcgcc gggtcgagga tccggacttc gttcccctca
     4981 agatcgcctg cgtcttctcc cctcccgcgg acggggacgc cgacgtgcgc cagctccagg
     5041 aggacctccc ccaggaaaag gccgacaacg agaaggaccc ggaccgcaag aaggccgccc
     5101 tccgttccat cctcgaggcc tacaattcct cctacggcac caaccactcc ctcggggact
     5161 tcgacctcta ctaccaggac gtccagaagc gcatcaaaga ccagaagttc cccaacgccg
     5221 acctcccccg caggggccgg gagaagatcg acctcaccat cgtggtggac atgcttctga
     5281 ccggcttcga ctcccagtat ctcaacaccc tctacgtgga caaaaacctc cggcaccacg
     5341 gcctcatcca ggccttctcc cggaccaacc gggtgctcaa cgccgcaaaa ccccacggcc
     5401 agattctcga cttccgccag cagcaggccg ccgtggacgc ggccatcgtc ctcttctccg
     5461 gggtggagag cgacaccaag gcccgggaga tctggctggt ggagcccgcg cccaaggtgg
     5521 tcgagaagct caccgccgcc cttagcaggc tggacgactt cctgcgatcc caggggctta
     5581 agcccgagcc cgagtccgtc cccgccctgc ggggcgacga ggcccgggcg gccttcgtcg
     5641 agcgcttccg ggaggtccag cgcctgaaga cccaactgga ccagtacacc gacatccccc
     5701 ccgaggccga ggccgccgtg gagcggctcc tgccccgaga ccggctcaac gccttccggg
     5761 gcgtgtacct ggagaccgcc cagcgcctcc gcgccgagcg ggaccgggac ggcgccgccg
     5821 gcgagagcgc cgcggaccag ctagacttcg agttcgtcct cttcgcttcc gcggacatcg
     5881 actacgacta catcatggcc ctgctcgccc ggttctccaa cgagagcccg gacaagctga
     5941 agatgagccg ggaccagctc atcggcctca ttcaggcgga cgccaagttc ctggacgagg
     6001 gcgacaccat cgccgactac gtccgcaccc tcagggccgg ccagggcctg gatcagaagg
     6061 agatcgaggc cggctaccgc cgattccggg acgagcgccg cgccgcggaa ctcgccggcc
     6121 tcgcccggaa gcacggtctt gcccccgacc gcctccggac cttcacggac gcgatcctgg
     6181 agcgcagggt cttcgacggg gaacgactca cggaactcct ggagccccgg agccttccgt
     6241 ggaaggaccg ccgggccgcg gagctggccc tcatggagga tctcgtcccc ctgctcaaga
     6301 agcgcgtcgg gggccgcgac atatccggac tgagcgcgta cgaatcgtga agaatcaagg
     6361 atccaggatg aacgacgaca agccccgcct cacgcccaag cttcgcttcc cggagttccg
     6421 ggatgcagag gggtggacag gaatgcggat gagtgaggtg tactcattca aaggcaacaa
     6481 ctcattttcc cgcgaccaac tcaattacaa tcagggtatc gttaagaata ttcactatgg
     6541 cgacatccat accaagtttt ccacactctt tgatgttacc tgtgagacgg tcccattcat
     6601 taatcccacg gagtcgttag atggtttccg tcctgataac gactgcgttg agggtgatat
     6661 tgttttcgcc gatgcgtcgg aagacatgga tgacatcggg aaaagcatcg aaattgttcg
     6721 attgaatggc gaacgtgtcg ttgccggtca acatacgatt ctagctcgac caaacgacga
     6781 catcctggtc attggatttg gtgcctacct tttcaaatca aaacacatcc gtactcaaat
     6841 ccaaaaagaa tcgcaaggtg caaaggtttt aggcctatcg gcatctcgtc ttactaaaat
     6901 catcctcccg atccccagaa gcaaggccga acaacataaa atcgccgcct gcctctcctc
     6961 cctggacgac ctgatcgacg ccgaaacccg gaaactcgac gcgctcaagg cccacaagaa
     7021 ggggcttctg cggaacctct tcccccggga aggcgaaacc cggccatggc tgaggttccc
     7081 ggagttccgg gatgcggggg agtggaagta cggcaaacta acagaactat gcgaaacaat
     7141 catggacggg acgcacttct ctccaaagac gaagattggc cctcggcctt atctcacatc
     7201 caaaaacatt cataatggta aaattgatct gtcgaatgtt tcatacattt ctgaagaaga
     7261 acacaatgca atttacaatc gttgcccagt gcaatggcac gacgtccttc tgacaaagga
     7321 tggcgctaat acaggcaatt gcgcccttaa tattctcgat ttcgagttct ccctactttc
     7381 aagcgttgct gtgctacgag gaaagcgaga ctcgctcgac caggagttct tgtttcactc
     7441 agtcttatct gactaccttc agacccagat catagattcg atgtcagggc aggctatcac
     7501 aagagtcaca cttgaaaaga tcggaaattt ctcgatagca gtaccctcaa ttcccgaaca
     7561 acaccgcatc gcctcctgcc tctcctccct ggacgacctg atctccgccc gaaccgacag
     7621 aatcgaagcc ttcaagaccc acaagaaggg gctaatgcaa cagctatttc cattctcgga
     7681 ggttaatcta tgaccctacc agagatagcc acattactta agcagagtga cgcaaagatt
     7741 attctggttt ttgggttcaa tgccgtcgga aagacacaac tttccgtcgc gtacaaaaac
     7801 gaaacgaaac tgaccgacgg aacacatact ggggtttatt acaacgcata cagcgaggat
     7861 ttgttcgtct ggaacaacga tgaagaaaat aatggtgcag atactcgact tattgttcag
     7921 ccaagcacgc ttagcaaatt tcactcttca ctaacagaag gcattattcg aaagaaactg
     7981 aaagcgtaca aaccaaagta tgatttcgag tttaccatgc atgatgatgt agagaagggc
     8041 atcaagtcca tctctttttt cattcccaat tccgaccttg atgttgctac tccctcgatt
     8101 aagatttcaa gaggagaaga acgtatattt gtgtggtgtt tctttttagc tctatttgaa
     8161 gtggaaggat gggcggatat aaattctagc cacttattta ttgacgaccc cgtgtccagc
     8221 ctagatgatc acaacatctt cattacgtca tctacgctat tcgaccttat agatgagtac
     8281 tatgagaaac ggaagataat tatagcaaca caccatattg gcttattctc gatcctattc
     8341 gattaccttc aaaaagggga aaaaagtgat aagtacaaac gttatacatg ccccatgatt
     8401 ctcagtagca aacatggcga tttaactctt gaaacttgca aaaaagatgt cttcctatac
     8461 cacttgcgct tgcttcagat actaaagcaa gcgcaagata tggatgaagt taaatcttat
     8521 cattttgtat tgcttcgaca ggtattagaa aatatggctt cttttctggg gacaggaaag
     8581 gttggccatg ttcttcaagt cattggtgtt gaggattctg acacatactc gcagaccgtc
     8641 aatacgctta gccacaagaa catttacttg tacgaatccg atgaactcgt agaagataat
     8701 aaaaggctat tttcagacat ccttgggaaa ctgatggatc aattcaagtt tgtattgcat
     8761 gcggagtgat tgatgaccga ttataaccac caacaactcg gcaagaccct ctgggccatc
     8821 gcggaccaac tgcggggcgc gatgaacgcg gacgatttcc gcgactacat gctgtccttc
     8881 ctcttcctgc gctacctctc ggacaactac gaggccgcgg ccaagaagga gcttgcccgg
     8941 gactaccccg ccatccccgc cagcgacccg cgcgtaccgc ttgcggtctg gtacgcgaac
     9001 aatcccgacg acatcgtgga cttcgaaaag cagatgcggc gcaagatcca ctacgtgatc
     9061 aagccggacc acctctggac caacatcgcc aacctcgccc gggaccagag ccccgacctc
     9121 ctggacaccc tgcgggacgg cttcaagtac atcgagaacg agtccttcga gagcaccttc
     9181 cagggcctct tctcggagat caaccttggc tccgaaaagc tcggccgcac ccacgccgac
     9241 aagaacaaga agctctgcga catcgtaaag gagatcgcca agggcctttc cgagttctcc
     9301 accgacgtgg actccctggg ggacgcctac gagtacctga tcggccagtt cgcagccggg
     9361 tcgggaaaga aggccggcga gttctacacc ccccagcaga tctccaccat cctctcccgc
     9421 attgtcacct tggacggcca ggagcccgcg accggcaagg ttccccgcct cgggagcgtc
     9481 ctggacttcg cctgcggctc cgggtccctg ctcctgaacg tgcgcaagca gatgggtccc
     9541 cacggcatcg gcaggatctg cggccaggag aagaacatca ccacctacaa cctcgcgcgc
     9601 atgaacatgc tcctgcacgg ggtcaaggac acggagttcg agatctacca cggcgacacc
     9661 cttaccaacg actgggactt cctgagggag accaacccgg cccggatgcc caagttcgac
     9721 gccgtcgtgg ccaatccccc cttcagctac cgatgggaac ccaaggagga catggccgac
     9781 gacgtgcgct tcaagaacca cggcctggcc cccaagtccg ccgcggactt cgcctttctc
     9841 ctccacggct tccactacct ctcggatcag ggcgtgatgg ccatcatcct gccccacggg
     9901 gtcctgttcc gcagaggagc ggaggaaaaa atccgcacca agctcttgaa ggacggccac
     9961 atcgacaccg tcatcggcct gcccgccaac cttttctatt ccaccggcat cccggtctgc
    10021 gttctggtgc tcaagaagtg caagaagccc gacgacatcc tcttcatcaa cgccgccggg
    10081 cacttcgaga agggcaagcg gcagaacgtg cttctcgacg agcacatcga aaagatcgtg
    10141 gatacctacc agcaccgccg ggaggaagcc cggtattccg cgcgcgtccc catgggccgc
    10201 atagcggagg aaggctacaa cctcaatatt tcccggtaca ttagcacggc ccaggaggag
    10261 cccgagatcg acctggcctc ggtccattcc gacctggtcc gtatcgagga cgagatcaag
    10321 acggcccagg acaaacacaa cgaattcctg aaagagctgg ggcttcccct actgccgccg
    10381 gggtgaggcg gagagaggac agaaagggac cgcaccaaat tatcgcataa atcagggggt
    10441 gagcgacatg gatcagaaac tgcttgacca ttgcattcaa gaatataagg aacgctccat
    10501 aacgaatccc gaggaatatc gccaggctcg ccaggagcga agcgagcgtg tcgcatggtt
    10561 ccaggcatgg aatcgtgcac gcctgctcac cctcaacgcc gattctcttg aggaatatct
    10621 ttccaggctt tgggccatgt tgatctgggg taataagcgc tactataccg atcttatcat
    10681 cgagaaaaat acactcgaag tcgttagaga taaccttgcc tcccttcttt atgacgacgc
    10741 tccagtcgca attaggtggg ataggtttcg agattgtgta tctcagatag gaccggcgat
    10801 ggttagcgaa ctgctcgccc attactatcc tagtcgtttc cccgtctgga accgcagagc
    10861 caaggaagcg ctcgaacgcc ttggggaaac aagtcttccg agatacgact atcagatcat
    10921 aggcgcattc tatgatcgac tgatccacct ggcttcaagt gtcgcagacg cgctttccaa
    10981 cgctggtttt gaagatgtgg atatgctctt cgttgattac ttcttttggg acctcgttca
    11041 agaaaagaac cccccggtta tcccatcagc cgttcaaagt caaacgcaag gtgcccagaa
    11101 tcaacaagat caggtattcg ttcataacga agtacgggac aagatcgccg agatcgggac
    11161 gtggcttgga ttcaagacaa gcacggaaca gaccgttacc acgggtaccc gcatcgatgt
    11221 tctgtgggag gcttcgatca gtaacatggg gcgcgtgctc tacgcatttg aggttcagac
    11281 ttcgggcagc attgacagcc ttcagctcaa cctcctccgc tctctctcga atccagccgt
    11341 acaggcggtt gtcgccgtga gcgatgaacg gcaacttgaa aaaataaaag cggaggctgc
    11401 gacacttccg gaactcaaga acaagctgag atgctgggat tcccgggaag tactggaaac
    11461 acatgccgct ctttccacag cattcgattc gatcaaccgg ctgaagcttg ttccggatgc
    11521 gttttagcgg ttgtacggca actatcgccc aatactcctc acgataacgg gtgccagatc
    11581 agcatcttaa ggaataacct ctctctttcg gcatcctccc ccctccacct ccacctccgc
    11641 ttgtagacaa atgtctacaa gcactctcgc attatccgtc gtacactttc ccctggtgtg
    11701 acacaacctc tgccggggcg cctccgtact ggttcgtcct gtataggtcg cagtaaaaat
    11761 caggaggttt tcatggctag acagaatctc gtttcggcgg tcctttccga cgaggtcaag
    11821 gcggacatcc tttccaagct ggcctcggtg cgcaagagcc tggggttttt gctgaccctc
    11881 cggcccgacc aggtgcagt
//
LOCUS       DAOTEI010000106        11751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3823_length_11751_cov_6.158687, whole genome shotgun sequence.
ACCESSION   DAOTEI010000106 DAOTEI010000000
VERSION     DAOTEI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11751
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3823_length_11751_cov_6.158687"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="P5313_10770"
     CDS             <1..527
                     /locus_tag="P5313_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY80888.1"
                     /translation="ADYRLLAATNRNLEALVAAGTFRSDLYYRLDVVPLSVPPLRERR
                     EDIPELVEHFAGRVAAAEGLDRPGTTEDFLAALKGYLWPGNVRELENLVERLVVLYRP
                     KVLEARLLEEEAPDRFGPMDPDARERTEILDALRRSGGNKSAAARSLGISRRALYYRM
                     SRLGIEAGDAPGND"
     gene            835..1863
                     /locus_tag="P5313_10775"
     CDS             835..1863
                     /locus_tag="P5313_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY80889.1"
                     /translation="MSLRNRIVALALVLILASPMAFAMGDKEGKEAKTVIVYSSVDEA
                     NAKKILDAFTADTGITAQFVHLSSGPALARLKAESANPQADVWMGAPSENHISAKTDG
                     LTQPHKGGNFSSLAPEFKDPDGYWRSFYMNPMAIAVNKDILARMGAPVPKSWNDLLNP
                     AYKGQIQMPSPQSSGTAYNIIASLVKLMGEDKAFEYLKKLGPNVQTYTSSGTGPSKGV
                     SVGQCAIALQFTPAFFEFIDQGFPMEVIFPSEGVWFEAPGVSIVKGARNPKTAEALVE
                     WLTSPKGQAVFTEAKTYFYPIIPGVKLGAGMPAFKSLKTIAVDPLWAAAEKKRLVDRW
                     VAEVLPVK"
     gene            1916..3607
                     /locus_tag="P5313_10780"
     CDS             1916..3607
                     /locus_tag="P5313_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY80890.1"
                     /translation="MTDRAIGASLGEIRRLVRDPLLAFVVAAVLAALAVFILYPLASV
                     IIVSLRPDGRWTLEIFRHVVSNPYLLQGFRNSILMGVLTAVIGTGIGFLFAFAVTRAD
                     VPFKRILHAVAIIPIVSPPFIGALAIIMLFGNNGFITWNLLRIRNFPIYGFTGLMIAQ
                     AVTFFPVAYLTLRGVLESINPTLEDAALDLGGSRFRVFRRVTLPLAMPGIASSMLVLF
                     IETLADFGNPLILAGSRFPILSVQAYLQITGMYDLPTGAALSVLLLVPSVTAWVIQKY
                     WVSRKRYVTITGKPTISSVKIVSPAVRSLIFAACLAVTAFILLIYASILWGALAKVWG
                     SDNTPTLRHFLYVFGVGFKAVRDTLTIAGLSTPASGLLGMVIAFLVIRKSFPGRKFME
                     FTSMLSFAVPGTVIGIGYILAFNKPPLVLTGTLLILLLNFVFRYVPVGIESGVAILQQ
                     IDPAIEEAAVDLGADARTTFSRVTLPMIVPAYFSGLVFAFVRAMTAISAAIFLVSSRW
                     NLMTVQIMAQVESGRLGAAAAFSVILVLIIFGAIFLIRALLKARYGRYGGSMLKF"
     gene            3619..4722
                     /locus_tag="P5313_10785"
     CDS             3619..4722
                     /locus_tag="P5313_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80891.1"
                     /translation="MSVRLDKVSKVFLDPSRPEGSVTAVDRVDLEVREGEFVTLLGPS
                     GCGKTTTLRIISGFEAPTRGDVFISGERVNDQPPNKRDTSMVFQSYAIFPHMSVEQNV
                     GFGLELKGRPKAEVREETARIMNVMGLTALAKRRPDQLSGGQQQRVALARAVINKPSV
                     LLFDEPLSNLDAKLRDQMRLEIRRIQQSFGITSIYVTHDQIEAMTVSDRIVVMEGGRV
                     MQIGTPFEVYSRPANRFVADFIGRVNFFPVTDASFSEEGLDADYRGVRIRAGSRNGDI
                     REGGAAVVAVRPESLRLEPESGSAARFLRGRIDKAVYLGAVVEYEIDAGLDRPVMAVS
                     HDPVVAGFFRVGDGVSLSFPPAAAHALAPEAVP"
     gene            4719..5378
                     /locus_tag="P5313_10790"
     CDS             4719..5378
                     /locus_tag="P5313_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY80892.1"
                     /translation="MTEAEGHIAGLLARRPELAGAESAIRALADLLTGCLRDGGTVLA
                     CGNGGSAADADHVACELAKGFLLSRPLPGPLRDSIEGNLERALGRTGAGLAGRLQVPL
                     RAVSLCSNSALLTAIANDQGPDAVFAQQVLGLGRKGDLILCLSTSGKSPSVLAAAAAA
                     RSLGLSAALLTGPDAAPPAGLFDVTVPVPGANAGEIQDLHRPVYHAVCAYAEAVLTAV
                     V"
     gene            complement(5395..5826)
                     /locus_tag="P5313_10795"
     CDS             complement(5395..5826)
                     /locus_tag="P5313_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY80893.1"
                     /translation="MSNNASIVLRDFEEEDFGSLSELWTETGIGNPARGDTLESVRRT
                     LALGGRLFVLAAGDRIAGSAWISDDGRRLYLHHMAVLPELQGRGLGKRLMEAAVSVAR
                     QRGMQMKLEVSEANPGAMALYRNYGFQTLEGYRTMIRRSTE"
     gene            complement(5920..7260)
                     /locus_tag="P5313_10800"
     CDS             complement(5920..7260)
                     /locus_tag="P5313_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HRY80894.1"
                     /translation="MEKRNVIIIGAAGRDFHNFNTYFRDKPEYNVVAFTAAQIPDIAG
                     RKYPAELAGKLYPKGIPIHLESDLPKLIKTLKVHECVFAYSDVPYTHVMGIGAIVNAA
                     GASFRLLGPADTQVKSTKPVIAVCAVRTGSGKSQTSRKIVQMLMAKGLKVVAIRHPMP
                     YGDLVAQKVQRFATVADLKKHKCTIEEMEEYEPHVVRGNVIYAGVDYEAILREAEKEA
                     DVILWDGGNNDFPFYKTDLLITVADPHRPGNEVSFYPGEVSLRMADVIVINKIDTAAP
                     DAVQTVRENIQRVNPSAVVIDGASPITVDKPELIKGKRVLCVEDGPTLTHGHMKIGAG
                     IVAARKFGAAELVDPRSAAVGKLAETFKIYPNIGTLLPAMGYGDQQVKDLEATINKTA
                     CDTVVIATPIDLNRIVKIKKPTVKVEYDLQEIGSPNLTEVLDEFLKKHKLGKKK"
     gene            7512..8756
                     /locus_tag="P5313_10805"
     CDS             7512..8756
                     /locus_tag="P5313_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HRY80895.1"
                     /translation="MSGLNITSEIGTLKRVILHTPGPEIEAMTPKEAEQDLYNDIIPL
                     EAVRREYDSLKAFLSTVASVYEVTDLLAQAIAPNGNKFEFLKDLAALCPIRNRMDELV
                     ALSPPDLARLTIEGLPALEKSLSAVLDPHAFAVRPLPNTYFMRDSAAVFGKYVVSCAT
                     AFDVRLIESVVTRFIFQHHPDFMAKGFLLNGPEERSRYITIEGGDFLVLGPTTLAVGV
                     SERSTPDAVERVAVNAARKLGEPVTVVAVQIPRERATIHLDMVFTMIDREACLVYAPV
                     VMDEGRCRAVRIQAQLNGKASFSEVQGLLPALKSAGFDLEPVLCGDGHRLFQDREQWL
                     SGANSFAFGPGKIVVYNCNAYTLDALTKAGFEYRKAEDFIQGSVKAEDFGRLVVGFDG
                     IELARGGGGARCMTCPVEREEP"
     gene            9057..10664
                     /locus_tag="P5313_10810"
     CDS             9057..10664
                     /locus_tag="P5313_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY80896.1"
                     /translation="MVDGGGKPADFIFLDVNPVFERISGLKKAEIVGRRAGEVLPDLL
                     RDEFDWIRFFGNVALDGVEAELERTFPPGKTLARIRAFPLGDPLTFAVFLDDPSSRTG
                     TAGRDPNAESRNAGEDTGPAYYFREAPDGIFLVDEQARYRDANPAALAMLGYERRELL
                     GRSIREILPPDQLEAGLRALETLKAQGRSSCELKLLRKDGGRIWVAVDAVRNPEGGFL
                     GYCKNITVRKYAEERNDLSSRAMEAIDQPVIITDSDGKILEANRAFLDLYGFRREEVL
                     GQNPRILNPGWETYVNLGYSYEDYVTLFDGMWRAMKDPSVGTWEGTVINRRNDGSLVW
                     VRLILNAVYDDRGGLKNLIGLPIDISESRSRETMSKVELYATIAAMAELRDEETANHM
                     RRVGIFSKIIAKSLGMPDKYCEDLELFAPMHDIGKVGVPDSLLLARRKLTHEEWTEMK
                     RHTVLGHNIVKDKQELALVAAITLRHHEKWDGTGYPDGVSGEEIPLSARITAVADVYD
                     ALRSERPYKTAWPHDRAAVEIVSQAGA"
     gene            complement(10779..11156)
                     /locus_tag="P5313_10815"
     CDS             complement(10779..11156)
                     /locus_tag="P5313_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRY80897.1"
                     /translation="MRTIRTEAAPIPAGHYSQAVVHGGVAYVAGLLPVEAGTNRKVFG
                     EIEEESAQVFRNLNAILAASGSSRDRVLRCTVYIADLELWGRFNAAYAAYFGEHRPAR
                     TVVPVPGLHYGFKVELDAIAAAD"
     gene            11243..>11751
                     /locus_tag="P5313_10820"
     CDS             11243..>11751
                     /locus_tag="P5313_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY80898.1"
                     /translation="MLPPLAPTADDIRAARARIAPYAHVTPLITSRTLDERTGRRVLL
                     KCENLQRVGAFKFRGACNAVLSLSDEEARRGVVTHSSGNHAQALALAARMRGIPARIV
                     MPRTAPAVKVAAVKGYGAEITFCEPTLEARTAAAEAIIAATGATLVHPYDDPRVIAGQ
                     ATCALEILE"
BASE COUNT         1930 a         4023 c         3745 g         2053 t
ORIGIN      
        1 gggcggacta ccgcctcctg gccgcgacca accggaacct cgaggccctg gtcgccgcag
       61 gaaccttccg gagcgacctg tactatcggc tcgacgtcgt gcccctctcg gtgcctcccc
      121 tgcgggaacg ccgggaagac attcccgaac tggtcgagca cttcgcaggg cgggtggccg
      181 ccgcggaggg cctcgatcgc ccagggacga cggaggactt tctggccgct ctcaaggggt
      241 acctctggcc ggggaacgta cgggaactgg agaatctggt cgaacgcctg gtggtactgt
      301 accggccgaa ggtcctggaa gcccgcctcc tcgaggagga ggcacccgac cggttcgggc
      361 ccatggatcc cgacgcccgg gagcggacgg agattctcga tgccctgcga agatccgggg
      421 gaaacaagag cgcggcggcc cggtccctgg ggatctcccg gcgcgccctg tactacagga
      481 tgagccgcct gggcatcgag gcgggggacg ctccgggaaa cgactgaacc ctcgctgcac
      541 acccgttgca cacctttgta cgcggcaccg tgaagacggc cgattcctcc cggggccggc
      601 ttccccgatt ccagcgggta ggacggagaa gtaccgtccc tgtccggggg aaaccgcggc
      661 ggaagcctga gaagactgcg gtttcttgcc gtaccctgcg gtcccttcct cggtactccc
      721 ggaaccgctt acgggggacg ggctccgccg gaccgtgaaa ctccggctga cgggtgcccg
      781 gaatttggca cgccccttgc tttatacatg gcaacccttt ctcgaggagg ctctatgtcg
      841 ctcagaaacc gtatcgtggc actcgccctg gtcctcatcc tcgcctcgcc catggcgttc
      901 gccatggggg ataaggaggg caaggaagcc aagaccgtca tcgtctactc cagcgtggac
      961 gaggcgaacg cgaagaagat cctggacgcc ttcaccgcgg acacgggcat aacggcccag
     1021 ttcgtccacc tttcttccgg ccctgcactg gcgcggctca aggccgaatc cgcgaatccc
     1081 caggcggacg tctggatggg cgcgcccagc gaaaaccaca tctccgccaa gaccgacgga
     1141 ctcacccagc cgcacaaggg gggcaacttc tcgtccctgg ccccggagtt caaggatccc
     1201 gacggctact ggcggagctt ctacatgaat cccatggcga tcgcggtcaa caaggacatc
     1261 cttgcccgga tgggagcccc cgtgcccaag tcctggaacg atcttctgaa cccggcctac
     1321 aaaggccaga tccagatgcc ctccccccag tcctcgggca ccgcgtacaa catcatcgcc
     1381 tccctggtga agctcatggg tgaggacaag gccttcgagt acctcaagaa gctcggaccg
     1441 aacgtccaga cctacacctc ctcgggcacc ggtccttcca agggggtatc cgtcggacag
     1501 tgcgcgatcg ccctccagtt caccccggcg ttcttcgagt tcatcgacca gggattcccc
     1561 atggaggtga tcttcccctc cgagggcgta tggttcgagg cccccggggt ctccatcgtc
     1621 aagggcgcca ggaaccccaa gaccgccgaa gccctggtcg aatggcttac cagtcccaag
     1681 ggccaggcag tcttcaccga agcgaagacg tacttctacc ccatcattcc cggcgtcaag
     1741 ctcggtgcgg gcatgcccgc cttcaagagt ctcaagacca tcgccgtcga tcctctctgg
     1801 gccgcggccg aaaagaagcg gctggtcgat cgctgggtcg ccgaggtcct tcccgtcaag
     1861 tgattccccc cggccgtccc gggaccctcc gggacggccg ccaaggagcg tacccatgac
     1921 cgaccgagcg atcggcgcct ccctggggga gatccgtcgg cttgtccggg atcccctgct
     1981 ggccttcgtc gtcgccgccg tcctcgcggc tctggcggtg ttcatcctct accccctggc
     2041 cagcgtgatc atcgtttccc tgcgcccgga cgggcggtgg accctggaga tcttccggca
     2101 tgtcgtctcc aatccctacc tgctgcaggg gttccggaac agcatcctga tgggagtcct
     2161 gaccgcggtg atcggcacgg ggatcggatt cctcttcgcc ttcgcggtca cccgggcaga
     2221 cgtccccttt aagcggatcc tccacgcggt ggccatcatc cccatcgtgt cgcctccttt
     2281 catcggcgcc ctggccatca tcatgctctt cgggaacaac ggcttcatca cctggaacct
     2341 cctgcggatc cggaacttcc ccatctacgg tttcacggga ctcatgatcg cccaggcggt
     2401 caccttcttt cccgtcgcct acctgaccct ccggggagtc ctggaatcga tcaatcccac
     2461 cctggaggac gcggccctgg acctgggggg atcccggttc cgggtcttcc gccgggttac
     2521 actgcccctg gccatgccgg gcatagccag ctccatgctg gtgctgttca tcgagaccct
     2581 ggcggacttc gggaatcccc ttatcctggc gggaagccgt ttccccatcc tctccgtcca
     2641 ggcctatctc cagataacgg gaatgtacga cctgcccacc ggagcggccc tctcggtcct
     2701 gctcctggta ccgtcggtca cggcctgggt catccagaag tactgggtct cccggaaacg
     2761 ctacgtgacc ataacaggga agcccacgat ctcctcggtg aagatcgtca gccccgccgt
     2821 gcggtccctg atcttcgcgg cctgcctggc ggtgaccgcg ttcatcctcc tcatctacgc
     2881 gtccatcctg tggggggccc ttgccaaggt atggggaagc gacaacaccc ccaccctccg
     2941 ccacttcctc tacgtcttcg gagtcgggtt caaggccgtc cgggacaccc tgaccatcgc
     3001 gggactctcc acccccgcct cgggtctcct gggaatggtc atagccttcc tggtcatccg
     3061 gaagtccttc ccgggccgga aattcatgga gttcaccagc atgctcagct tcgcggtgcc
     3121 cggtaccgtc atcggcatcg gatacatcct tgccttcaac aagcctcccc tggtgctgac
     3181 gggcacactc ctgatcctgc tcctcaactt cgtgttccgc tacgtgcccg tgggcatcga
     3241 gtccggggtc gcaatcctcc agcagatcga cccggccatc gaggaggccg cggtggacct
     3301 gggggcggac gcccggacga ccttctcccg ggtgaccctt cccatgatcg tgcccgccta
     3361 cttctccggc ctggtgttcg ctttcgtccg ggccatgacg gccatcagcg cggcgatctt
     3421 cctggtctcc tcgcggtgga acctcatgac cgtccagatc atggcccagg tggaatccgg
     3481 ccgcctcggg gccgcggcgg cgttcagcgt gatcctggtc ctgatcatct tcggggccat
     3541 cttcctgata cgcgccctgc tcaaggcccg gtacggccgc tacggcggct cgatgctgaa
     3601 attctaggga ggatccgtat gagcgtgcgt ctggacaagg tgagcaaggt cttcctggat
     3661 ccctcccggc ccgaaggctc ggtgaccgcc gtggaccgcg tggacctgga agtccgggag
     3721 ggggagttcg tcaccctcct gggcccctcg ggctgcggca agacgacgac cctgaggatc
     3781 atctcgggat tcgaggcccc cacccggggg gacgtcttca tctccggaga gcgggtcaac
     3841 gaccagcccc ccaacaagag ggacacctcg atggtgttcc agagctacgc gatcttcccc
     3901 cacatgtccg tggaacagaa cgtcggcttc ggcctggagc tcaagggtcg gcccaaagcc
     3961 gaggtccggg aggagaccgc gcggatcatg aacgtgatgg ggctgacggc cctggccaag
     4021 cggaggccgg accagctctc cggaggacag cagcaacggg tggccctggc ccgggcggtc
     4081 atcaacaagc ccagcgtcct tttgttcgac gaacctcttt cgaacctgga cgccaagctc
     4141 cgggaccaga tgcgcctgga gatccgccgc atccagcaga gcttcggcat cacgagcatc
     4201 tacgtcaccc acgaccagat cgaggccatg acggtctcgg accggatcgt cgtcatggaa
     4261 ggcggccggg taatgcagat cggaaccccc ttcgaggtct actcgaggcc cgcgaaccgc
     4321 ttcgtggcgg acttcatcgg cagagtgaac ttctttccgg tcacggacgc ttccttctcg
     4381 gaagaggggc tggacgcgga ctaccggggc gtccgtatcc gggcgggatc ccggaacggg
     4441 gacatccggg agggaggagc ggcggtcgtc gcggtgcggc ccgagtccct gcgcctcgag
     4501 cccgagtcgg gttcggcggc ccgcttcctt cgggggcgta tcgacaaggc cgtatatctc
     4561 ggcgccgtgg tcgagtacga gatcgacgcg ggcctggacc gccccgtgat ggcggtgtcc
     4621 catgatcccg tggtcgcggg attcttccgg gtgggcgacg gggtttccct gagcttcccc
     4681 cccgcggcgg cccacgccct ggctccggag gccgtcccgt gacggaggcg gaagggcaca
     4741 tcgccgggct cttggcccgc cggcccgagc tcgcgggggc ggaatccgcg atccgtgcct
     4801 tggcggacct gcttaccggg tgcctccggg acggggggac cgtcctggcc tgcggcaacg
     4861 ggggatccgc cgcggacgcg gaccatgtag cctgcgaact ggccaagggg ttcctcctct
     4921 cccggcccct cccgggtccg ctccgggact ccattgaggg gaaccttgaa cgggctttgg
     4981 gccggaccgg cgccggcctg gccgggcgcc tccaggtccc cctccgggcc gtctccctgt
     5041 gttcgaactc ggccctgctc acggccatcg ccaacgacca gggcccggac gccgtgttcg
     5101 cccagcaggt cctcgggctg ggccggaagg gggatctcat cctctgcctg agtacctccg
     5161 ggaaatcacc ctccgtcctg gcggccgccg cggccgccag gtccctcggg ctttccgcgg
     5221 ccctactcac gggaccggac gccgcgcccc ccgccggcct tttcgacgtg acggtccccg
     5281 tacccggcgc gaacgccggg gagatccagg acctgcaccg cccggtctac cacgcggtct
     5341 gcgcgtacgc ggaggcggtc ctgaccgccg tagtttgacc cggccttcgt gcccctattc
     5401 cgtactcctc cgtatcatgg tccggtaccc ctccagggtc tggaacccgt agttccggta
     5461 cagggccatg gcccccgggt tcgcctcgga gacctccagc ttcatctgca tcccgcgctg
     5521 ccgggcgacc gagacggccg cctccatgag gcgcttcccg aggccccggc cctggagttc
     5581 cggcaggacg gccatgtggt gcaggtagag ccggcggccg tcgtcggaga tccaggcgga
     5641 tccggcgatc cggtcccccg cggccaggac gaaaagccgc ccgcccagag ccagggtgcg
     5701 gcggacggac tcgagggtgt cgcccctcgc cgggttgccg atgcccgttt ccgtccagag
     5761 ctccgagagg gatccgaagt cctcctcctc gaaatcccgc aggacgatgg acgcattgtt
     5821 cgacatgcaa aatcctcccg gccgcgccaa agaaccgggg ccgccccttc ggacggcccc
     5881 gcgaattcgc gggccggatg cccggcccgc tcgggctgct tattttttct tccccagctt
     5941 gtgcttcttg aggaactcgt ccaggacctc cgtcaggttg ggactgccga tttcctgaag
     6001 atcgtattcc accttgaccg tgggcttctt gatcttgacg atgcggttga ggtcgatggg
     6061 ggtggcgatc acgacggtgt cgcaggcggt cttgttgatc gtggcctcca ggtccttgac
     6121 ctgctggtcc ccgtagccca tggcgggcag gagggtgccg atgttcgggt agatcttgaa
     6181 ggtttccgcc agcttgccca ccgcggcgga ccggggatcc accagctcgg cggcgccgaa
     6241 cttgcgggcc gccacgatgc cggcgccgat cttcatgtgc ccgtgggtca gggtcgggcc
     6301 gtcctccacg cagaggacgc gctttccctt gatcagctcg ggcttgtcca cggtgatggg
     6361 gctggccccg tcgatgacga ccgcgctcgg gttcaccctc tggatgttct cccggaccgt
     6421 ctggacggcg tccggagccg cggtgtcgat cttgttgatc acgataacgt cggccatgcg
     6481 cagggagacc tcgccggggt agaacgacac ctcgttgccg ggccggtggg gatccgcgac
     6541 ggtgatcaaa aggtcggtct tgtagaaggg gaaatcgttg ttcccgccgt cccagaggat
     6601 gacgtccgcc tccttctccg cctcccggag gatggcctcg tagtcgacgc ccgcgtagat
     6661 cacgttgccc cggaccacgt ggggctcgta ctcctccatc tcctcgatcg tgcacttgtg
     6721 cttcttgagg tccgcgacgg tggcgaagcg ctggaccttc tgggccacca ggtcgccgta
     6781 gggcatgggg tggcggatgg ccacgacctt gagccccttg gccatgagca tctggacgat
     6841 cttgcggctg gtctggctct tgccggagcc ggtccgcacg gcgcagaccg cgatgacggg
     6901 cttggtggac ttgacctggg tgtccgcggg cccgaggagc cggaaggagg cgcccgcggc
     6961 gttcactatg gcgccgatgc ccatcacgtg ggtgtagggc acgtccgaat acgcgaagac
     7021 gcactcgtgg accttgaggg tcttgatgag cttgggaagg tcggactcca ggtggatggg
     7081 gatgcccttc ggatacagct tgcccgcgag ctcggcggga tattttctgc ccgcgatgtc
     7141 cgggatctgg gcggccgtga aggccaccac gttgtactcc ggcttgtcgc ggaagtacgt
     7201 gttgaagttg tggaagtcgc gtccggcggc tccgatgatg atgacgttcc tcttctccat
     7261 gacaaggacc tgccttttat gatcgaggcg gatcgggcgc caggttcgct tcgcggatcg
     7321 gatcgtccgg ttcgtcccga cggactagcg ccgggggatc cggcgcgggc aaagtatgcg
     7381 cccgtgaaaa aacggtgtca agaaaaaacg acgtaaatat ttatacggaa agaaggtata
     7441 atcattccaa agacccgggc ccttcccatg cgcggggccc ctggggtaca atccgcccga
     7501 aaggcggagc catgtccgga ctcaacataa ccagtgaaat cgggacgctc aagcgcgtga
     7561 tcctgcacac ccccggcccg gagatcgagg ccatgacccc caaggaggcg gagcaggacc
     7621 tctacaacga catcatcccc ctggaggcgg tgcgtcggga gtacgacagc ctcaaggcgt
     7681 tcctgtccac cgtagcctcg gtgtacgagg tcacggatct cctggcccag gccatcgccc
     7741 cgaacggcaa taagttcgag ttcctgaagg accttgccgc cctctgcccc atccgcaacc
     7801 ggatggacga actcgtggcc ctgtcccccc cggacctggc ccgcctgacc atcgaaggac
     7861 tgcccgccct ggagaaaagc ctttccgcgg tcctggatcc ccacgccttc gcggtccggc
     7921 ccctgccgaa cacctacttc atgcgggact ccgcggcggt gttcgggaaa tacgtcgtgt
     7981 cctgcgccac ggccttcgac gtccgcctga tcgagagcgt ggtcacccgc ttcatcttcc
     8041 agcaccaccc ggatttcatg gccaagggct tccttctgaa cggaccggag gagcgcagcc
     8101 ggtacatcac catcgagggc ggggatttcc tggtccttgg gccgacgacc ctcgccgtcg
     8161 gggtttccga gcgcagcacc ccggacgccg tggagagggt ggcggtcaac gcggcccgca
     8221 agctggggga acccgtcacg gtggtggcgg tccagatccc ccgggagcgg gcgaccatcc
     8281 acctggacat ggtcttcacc atgatcgacc gggaagcctg cctggtctac gcccccgtcg
     8341 tgatggacga gggccgctgc agggccgtcc ggatccaggc ccagctcaac ggcaaggcct
     8401 ccttctcgga agtgcaggga ctcctgccgg ccttgaagtc cgcgggcttc gacctggagc
     8461 ccgtgctctg cggggacggc caccgactct tccaggaccg ggagcagtgg ctctccgggg
     8521 ccaactcctt cgccttcggt cccgggaaga tcgtcgtgta caactgcaac gcctataccc
     8581 tggacgccct gaccaaggcc ggcttcgagt accggaaggc cgaggacttc atccaaggtt
     8641 ccgtgaaggc cgaggatttc ggccgcctgg tggtgggctt cgacggcatc gaactggccc
     8701 ggggcggggg cggcgcccgc tgcatgacct gcccggtgga gcgggaggag ccgtaggaac
     8761 gggaatccgt cggaatactc cgattccggg tcgagcttcg tctttccttt ttccttcaga
     8821 attgttatgg taacgatgtc gagtttgtcg gttcgcggcc cgtccgcacc gtacagcgaa
     8881 ttcatccaag ttaggtagat tttctcccgg gaactatcgt cgggagaagt attcgacgtc
     8941 cgtgccctgc acggacgaat ccgacctgga aggatttgag ggaacacatg aaccgggaac
     9001 tctggcggtc ttcttccctg gatcgcgcgc ccattggata ctcctgccac cgcttgatgg
     9061 tcgacggcgg cggcaaaccc gcggatttta tcttcctgga cgtcaacccc gtcttcgaac
     9121 gcatcagcgg cctgaagaag gccgagatcg tgggccgcag agccggcgaa gtcctgcccg
     9181 acctgctgcg agacgagttc gactggatcc ggttcttcgg gaacgtcgcc ctggacgggg
     9241 tcgaggccga gctcgagcga accttccctc ccggcaagac cctagcacgg atccgggcct
     9301 tccccctggg cgacccgcta accttcgcgg tcttcctgga cgacccgtcc tcccggaccg
     9361 gcaccgcggg cagggacccg aatgcagaat cccggaacgc cggagaggac accggaccgg
     9421 cctattactt ccgggaagct ccggacggga tcttcctggt ggacgagcag gcccggtaca
     9481 gggacgcgaa ccccgcggcc ctggccatgc tcggctacga gcggcgggaa ctcctcggcc
     9541 gctcgatccg ggagatccta cccccggacc agctcgaggc ggggcttcgg gccctcgaga
     9601 ccttgaaagc ccagggccgg agctcctgcg aactgaagct cctccgcaag gacgggggcc
     9661 gtatctgggt ggccgtggac gcggtacgga atccggaggg tggattcctg ggatactgca
     9721 agaacatcac cgtccggaag tacgcggagg aacggaacga cctgtcctcc cgggccatgg
     9781 aagccatcga ccagcccgtc atcatcacgg attcggacgg gaaaatcctg gaagccaacc
     9841 gggccttcct ggatctgtac ggatttcgcc gggaagaggt cctgggccag aacccccgga
     9901 tcctgaatcc cgggtgggag acctacgtca acctcgggta ttcctacgag gattacgtga
     9961 ccctgttcga cgggatgtgg cgggccatga aggacccctc cgtcgggacc tgggagggca
    10021 ccgtcatcaa ccggcgcaac gacgggagcc tggtctgggt ccgcctcatc ctcaacgcgg
    10081 tctacgacga tcggggcgga ctgaagaacc tgatcggcct tcccatcgac atctccgaga
    10141 gccggtcccg ggaaacgatg tccaaggtgg agctctacgc caccatcgcg gccatggctg
    10201 agctccggga cgaggagacc gcgaaccaca tgcgccgggt cgggatcttc tccaagatca
    10261 tcgcgaaatc cctgggcatg ccggacaagt actgcgagga cctggagctc ttcgccccga
    10321 tgcacgacat cggcaaggtg ggagtcccgg attccctgct cctggcgcgt cggaagctca
    10381 cccacgagga gtggacggag atgaagcgcc acaccgtcct gggccacaat atcgtgaagg
    10441 acaagcagga actcgccctg gtggcggcca tcaccctgcg gcaccacgag aagtgggacg
    10501 gcaccggata tcccgacggc gtgtccgggg aggagatccc cctgtccgcc cgaatcaccg
    10561 ccgtcgcgga cgtctacgac gccctgcgga gcgaacgccc ctacaagacc gcctggcccc
    10621 acgacagggc ggccgtggag atcgtctccc aggccggcgc gtagttcgat ccccggatcg
    10681 tgcgggtttt tgaggacaac caggatctct tcgcccggat cttcgaggaa ctggaataac
    10741 accggttccc gccaagacgg ctcgatccgc gcttcctgct agtccgccgc cgcgatcgcg
    10801 tccagctcca ccttgaaccc gtagtggagg cccggcacgg gcaccaccgt gcgagcgggc
    10861 cggtgctccc cgaagtaggc ggcgtaggcc gcgttgaacc ggccccagag ttccaggtcg
    10921 gcgatgtaca cggtgcagcg caggacccgg tcccgggaac tccccgaggc ggccaggatc
    10981 gcattcaggt tgcggaagac ctgggcggac tcctcctcga tctctccgaa gacctttcgg
    11041 ttcgtgccgg cctcgacggg caggagcccc gccacgtagg cgactccgcc gtgcaccacg
    11101 gcctgggaat aatgccccgc ggggatgggc gcggcttccg tccggatcgt gcgcatggga
    11161 cctcccgtcc ttcaattctt cgaggatgat tccgcacttt acccgattcc gcggtccgcg
    11221 tatactcggt ttggaggaac ccatgctgcc cccactcgca ccgaccgcag acgacatccg
    11281 cgccgcccgg gcccggatcg ccccctacgc ccacgtaacc cccctcataa ccagccggac
    11341 cttggacgaa cggacaggcc gccgggtcct gctgaaatgc gagaacctcc agcgggtggg
    11401 ggcgttcaag ttccggggcg cctgcaacgc ggtgctgtcc ctgtccgacg aggaagcacg
    11461 ccggggggtc gtgacccatt cctcgggaaa ccacgcccag gccctggccc tggcggcccg
    11521 catgcggggc atcccggccc ggatcgtcat gccccgcacg gctccggcgg tcaaggtggc
    11581 cgcggtcaag ggctacgggg ccgagatcac cttctgcgag cccaccctgg aagcccggac
    11641 cgccgcggcg gaggcgatca tcgcggccac cggggcgacc ctggtccacc cctacgacga
    11701 tccccgggtg atcgcgggcc aggcgacctg cgccctggag atcctggaat t
//
LOCUS       DAOTEI010000107        11620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3885_length_11620_cov_4.903156, whole genome shotgun sequence.
ACCESSION   DAOTEI010000107 DAOTEI010000000
VERSION     DAOTEI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_11620_cov_4.903156"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5313_10825"
     CDS             complement(<1..304)
                     /locus_tag="P5313_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY80899.1"
                     /translation="MNEATPANAAAGNPPAAGTSAAGLAGALTVNGIRRQVRQEEAEL
                     TLLEYLREVLGLTGTKNGCAVGACGSCTVVVNGQGRRSCKTRVSEVLGKDVVTIEGL"
     gene            complement(301..2559)
                     /locus_tag="P5313_10830"
     CDS             complement(301..2559)
                     /locus_tag="P5313_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY80900.1"
                     /translation="MANETKSAFRNDARAKVRGRALYADDIRLPDMAHAVPVYTRFVR
                     ARNLRVDVSAARAAPGVLGVFTAADVPGQVRFGQIVRDYPMLAEGTIQCEGEVIALAV
                     AETRRQALAAAALVVEHAEELPPVTDPEEALAPGAPVVRWGGDDNLANRGRVRRGDAA
                     AALASCDLVVDETFSTQAVEHAYMEPESAVAIPRPDGILEIRGSMQHPFSTRRFVAAL
                     LGVSLAEVEVVGTTAGGGFGGKDDTAAIVCARAALAARKLGRPVKLTYDREWSFRESY
                     KRHPYRLRYRVGFSKDGTARAAEIDMLCDSGAYTSVTPWVNWRSTAQCCGPYYVDAVR
                     MDIRAAATNNVFTGAMRGFGAPQVNFAVEQVMDIAAERLGLSPLEIRRKNMVKQDGLT
                     ITGQRLDTHVVSLGQVMDTVVRDSGYEAKLARCSRGKAEDGRLYGIGLAISYRGSSLG
                     AEGLDYCSAVVNVQADGSVLLDAAIHENGQGAESVQMLILARELGLPVERIRRQWSST
                     SRIPDGGTTVATRGTIMGGGAVAQAARELKRLMAETLADELGDPKAVRFEGGRVGAPG
                     GPSLSWEEAVSRMYDRRIYPYAFGTFRAPPVSWDEHTGQGDAYFTYVYSCQAAEVSVD
                     PATGRVQLLALWAAHDVGRAINPPMTLGQAYGGMVQAAGMALMEDLAPEGGRLGNRNL
                     DRYKIPRARDIPEMRCTLVENPDPNSPTGAKGIGEPAIELLAPAIANAVYNATGIRCR
                     DLPIRLDPEALK"
     gene            complement(2606..3334)
                     /locus_tag="P5313_10835"
     CDS             complement(2606..3334)
                     /locus_tag="P5313_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80901.1"
                     /translation="MPIEQIPPEDETALGPDRYRDSALFRAIESSKPDVLVADIHWFA
                     LDSFLRDLPCKKVFLTRQIDPRTFRFRTPDRELVFRPEDWDLVLRSEPGFDLPFPSES
                     VEPLVLRNRDEILPAEAARADLGLGPEDRACLFAFSGRTGEGAEAWKSFSYLEEEGWT
                     VVRSDNREGGLFPAMDWYNAFDLVVCGAGYNQFWEARYLRKEAYFVPFPRRFEDQARR
                     IALCSDYEFEENGADQVVRRILAL"
     gene            complement(3497..4816)
                     /locus_tag="P5313_10840"
     CDS             complement(3497..4816)
                     /locus_tag="P5313_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80902.1"
                     /translation="MKRRYATLAAAAAALLTLASCELALGPRAEGTIILNLGGGPVSR
                     AFSDAPFSGLPVFSSVTVSVSGSGMPTASQTVPGNAGSLTIQVPAGPARKVEVYAVPD
                     WAATADIIFDPLPTLAKAYGGTAVVDVAGGQKVNLSMDMGVVETKILLPSAYSSLLYG
                     LDIGDQNPVSIDLAGNVILQNSDFEFDKFGRLYFSANDGSGVGRVFRLDDPSSTPFWI
                     ENYTSPGRITIDNVHDRVFLASLSTSYYDLVDGSSVIINLPAGHSSPGDSRIIAVDPA
                     GYIYSNANYNNQDWVFKYSLGPIYGAGAPMSDAQSLVGRTYGELGLSSSILPKDAVVK
                     DGHLYIAAAEHLTPNQRGKILKLSLSDLSMIDEIGWSSGTPSSPSSQFYGPSRFLAIT
                     PRKLIVSDEGFDGSNLDRIVEVDLDTGFVSKLQVTTGVVSFFESYFC"
     gene            complement(4828..5523)
                     /locus_tag="P5313_10845"
     CDS             complement(4828..5523)
                     /locus_tag="P5313_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRY80903.1"
                     /translation="MKRILAISILLAAAVLAAAQAAAPLAVIRQVAGTVEIKAPGRDW
                     VPARAGAALTKDTLISTAFKSVAVISVGTSTLTVKPLTRLSLEEIARLEGSETVRLAL
                     LSGRVRAEVKPPAGGKTDFTVKSPTATASVRGTGFDQDPVNLDVDDGNVEFTGPDGLP
                     VQVGAGQSSTVGEDGGAVPPKDMPDQALSPPPPAGLDGSILSFVQGLVDNSGASWGTV
                     DLRFNPWVGAPID"
     gene            complement(5538..6719)
                     /locus_tag="P5313_10850"
     CDS             complement(5538..6719)
                     /locus_tag="P5313_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80904.1"
                     /translation="MIRRRTLLPLAAAAVLLIPAASAGASDFGTALSLAPKVSQEGFS
                     LTVMANPWYSALLGENGELYFSGIVKLETTFQSATLLLDLGRSQVKLRPSPGISWEFG
                     RMPFADESGFVASGLLDGARWTGSLGSLGASAGLFYTGLLNKKSAEIALTAADGADLL
                     DADRFFAPPRLLAVLSGGLRNPPAFLSRLSAQAVGQFDLRGGSDTLHSQYLTLTAGIP
                     AGDAFGFEASAAAALAEPSGSDPVFHAAGALQAAWDPPGALKDQAYLGLRAATGADGG
                     FPNITSLEQGRVFTPGPSGLASARLGYKVRPSAVLGAEVQAVGFLRFAEGGPADAELD
                     PGSDSAWLGAELYASLTWTPLSDLSVLAGTGIFLPASGGAFLPDTPARWLLSVAAVLS
                     L"
     gene            complement(6716..7162)
                     /locus_tag="P5313_10855"
     CDS             complement(6716..7162)
                     /locus_tag="P5313_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80905.1"
                     /translation="MRKTFPVLFLAAAAALYAQSAKEMDLLLADPGVSYARAARYILP
                     AAGILPAAVSEADAYKAALERGWLPDGADPAAPIRLDESAYLILRAFSLEGGLMYRLF
                     PGPRYAYRELVYRQFIQGRRDPARGLPGERLVRILGRVLDAKGGGE"
     gene            7337..10063
                     /locus_tag="P5313_10860"
     CDS             7337..10063
                     /locus_tag="P5313_10860"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY80906.1"
                     /translation="MKNQRAPALAAIGSAVLFSALYLTSFFRSAESGVYDLFLGLKPP
                     RPRTDRVALLDVDDRAIAHVGVFPWPRSVVADGLLRLKEFGAAAVVFDIEYLDSSPPG
                     IDAVYLQRGLPRDFQRSFGDIGTNVSDLFNALTSGQISPREARGYAAQLADLIASERD
                     GLLARARGLAQDNDRYLGQAARLNGRVWATVNLQTVKLEGEQAERRAGARERFSVTVE
                     AAPEAPAGDYVDVLAPIPYFLEAARGAGFTNVVIDPDGVRRRISLTRRVDGAWYLQLV
                     TAPLLEALGNPKLVLERRRLRLLGASLPEGGTGDIDIPLDPEGHMLIDWPRENYFESY
                     DHVSFYSLSRLEELEAALGESVSALASLESWFLPDPEGELVLAYATLAGLTETLDASE
                     ASLRTALESASAEDFDQWRLGRQEARSGLKDLLASGAVDTLRRTAEATAASARPDEAA
                     ALKAEAETAGKLASGLASILAEIEEIRTRLDAAFAGKIVIIGRVDTGTTDIGVNPFHG
                     EYINVGTHAAVADTILARTFIRPVGSWASILLALLAAPLFVLTMGRFKAGVRIAVGLG
                     GAALVFWGSYAIFAAARIFVGPLGATLAVILASVVREAVDFVSTEREKRFLRKAFDTY
                     VSPEYVNELVAQPGSLKLGGSKRWMTALFTDVRGFSTISEQLDPEALVGLLNRYLSTM
                     SDIILEERGTIDKYEGDAIIAFFGAPLTLQDHARRSLRSAILMKRAEARLNEAVLAEG
                     LTPAPLATRIGINTGDMVVGNMGTEKKMNYTMMGNAVNLAARLEGVNKQFNSWILTTE
                     ATVKEAGPEFLVRRTARVRVVGIFEPVRLYEVLDFVSEAPEKRKTTVELFHRALEVFE
                     AKDWTEAGKLFLKVLSHDPEDGPSQSYLARLDGFRKKPPAKDWDGVFNLTEK"
     gene            10065..>11620
                     /locus_tag="P5313_10865"
     CDS             10065..>11620
                     /locus_tag="P5313_10865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY80907.1"
                     /translation="MSREGEKGLCRRILEAANDGVIVTDPDGAILEANPAFLAMTGYG
                     MDEVAGKNARILNSGRHDAEFYQSLWAEVRSAGAWTGEIWNRRKSGEIYPCRVKIQAV
                     PDEAGEASGFIGIFSDLSPLKDSEERLRNAVLFDPLTGLPNRSLFRDRLAQILDRARK
                     KATRAALVFVDLDRFKHINESLGYQAGDELLREAARRLTERTGGAGTVCRLGGDEFAV
                     ILDTVQRSEDAGAAAAGILAALSAGFPMGPSEVHTGASAGIAVFPFDGTDAGALEKKA
                     EAAMYEAKEAGRGRYRFASGKVGHTSRRQLELESRLRRALERGEFYLTYQPQAATGGV
                     VPGTGAGLMGAEALIRWKPEGADTVSPGDFMDIAESTGLIVPMGEWALKTACADAKLW
                     NDLGRPLTVSVNVSPRQFQAGTLARVTREALDASGLDPGRLKLEITETLLMDHMGETV
                     TVMNEIYPQPSLPENAAEGIVRGLYRCGGRGDPDAPVRVRLLGSGTILPQVIAAADLL
                     AERYGVASDVL"
BASE COUNT         1936 a         3966 c         3954 g         1764 t
ORIGIN      
        1 ccaggccctc gatggtcacc acgtccttgc ccaggacctc ggagacccgg gtcttgcagg
       61 accggcgccc ctggccgttc accactaccg tgcaggatcc gcaggccccc acggcgcagc
      121 cgtttttggt ccccgtgagc ccgaggacct cccgcaggta ttccaggagg gtgagctccg
      181 cctcctcctg ccggacctgc cgacggattc cgttgacggt cagggcgccg gcgaggccgg
      241 cggcagaggt cccggcggcg gggggatttc cggccgccgc gttcgcgggt gtcgcttcgt
      301 tcatttcagg gcctccgggt cgaggcggat ggggaggtcc cggcagcgga tgccggtcgc
      361 gttgtacacc gcgttggcta tcgcgggagc cagcagctcg atggccggct ccccgatgcc
      421 cttggcgccg gtgggcgagt tcgggtcggg gttctccacc agggtgcacc gcatttccgg
      481 aatgtcccgg gcccggggga tcttgtagcg gtccaggttc cggttgccga gccggccgcc
      541 ttccggggcc aggtcctcca tcagggccat gcccgcggcc tggaccatgc ctccgtaggc
      601 ctgccccagg gtcatgggcg ggttgatcgc ccggcccacg tcgtgggcgg cccagagggc
      661 caggagctgg accctgcccg tggccgggtc cacggagacc tccgcggcct ggcaggaata
      721 gacgtaggtg aaataggcgt ccccctggcc cgtgtgctcg tcccaggata ccgggggtgc
      781 ccggaaggtg ccgaaggcgt aggggtagat ccggcggtcg tacatccggg ataccgcctc
      841 ctcccaggag agggaaggtc cgcccggagc gcccacccgg ccgccctcga agcggaccgc
      901 cttcggatcg cccagctcgt ccgccagggt ttccgccatg agccgtttca gttcccgggc
      961 cgcctgggcc accgcgccgc ctcccataat agtcccccgg gtggccaccg tggtgccccc
     1021 gtcggggatc cgggaggtgg aggaccactg gcgccggatc cgctccaccg ggagccccag
     1081 ttcccgggcc aggatgagca tctgcacgga ctccgcgccc tggccgttct cgtggatggc
     1141 cgcgtccagc aggaccgacc cgtccgcctg gacgttcacc accgcggaac aatagtccag
     1201 gccctcggcc cccaggctcg agccccggta actgatcgcc aggccgatgc cgtagagtcg
     1261 gccgtcctcg gccttccccc gggagcaccg ggccagcttg gcctcgtacc ccgagtcccg
     1321 gaccaccgtg tccatcacct ggcccaggct gaccacgtgg gtgtccaggc gctggcccgt
     1381 gatcgtgagg ccgtcctgct tgaccatgtt cttccggcgg atctccaggg gcgacagccc
     1441 caggcgctcc gccgcgatat ccatcacctg ctccaccgcg aagttgacct ggggggcacc
     1501 gaagccccgc atggccccgg tgaagacgtt gttcgtggcc gccgcccgga tgtccatgcg
     1561 caccgcgtcc acgtagtagg ggccgcagca ctgggccgtg ctccgccagt tcacccaggg
     1621 ggtcacgctg gtgtaggccc cggagtcgca gagcatgtcg atctcggccg cccgggccgt
     1681 gccgtccttg gagaagccca cccggtaccg caggcggtac gggtggcgct tgtagctctc
     1741 ccggaaggac cactcccgat cgtaggtcag cttgaccggc cggccgagct tccgggccgc
     1801 cagggccgca cgggcgcaca cgatggccgc cgtgtcgtcc ttgcccccga agcccccgcc
     1861 ggcggtggtg cccaccacct ccacctccgc cagggatacc cccaggaggg ccgcgacgaa
     1921 gcgccgggtc gagaaggggt gttgcatgct cccccggatt tccaggatac cgtcgggccg
     1981 ggggatggcc accgccgact ccggctccat gtaggcgtgt tcgacggcct gggtggaaaa
     2041 ggtttcgtcg accaccaggt cgcaggaggc cagggccgcc gcggcgtccc cccggcgcac
     2101 ccggccccgg ttggccaggt tgtcgtcccc gccccagcgg accaccgggg cgcccggggc
     2161 cagggcctcc tcggggtccg tcaccggagg gagctcctcc gcgtgctcga ccacgagggc
     2221 cgccgcggcc agggcttggc gccgggtctc ggcgaccgcc agggcgatca cctcgccctc
     2281 gcactggatc gtcccctccg ccagcatcgg gtagtcccgg acgatctggc cgaaccggac
     2341 ctggccgggc acgtccgcgg cggtgaagac ccccaggacc ccgggggccg cccgggcggc
     2401 ggagacgtcc acccggaggt ttcgggcccg cacgaagcgg gtgtagaccg gcacggcgtg
     2461 ggccatgtcc ggaagccgga tatcgtccgc gtacagggcc cggccgcgta ccttggcccg
     2521 ggcgtcgttg cggaaggctg acttggtttc gttcgccata ggaggcatcc tttatgacac
     2581 gggccgtcgg atttgtaaag ccgactcaga gcgccaggat ccgccgtacc acctggtccg
     2641 ccccgttctc ctcgaactcg tagtccgagc agagggcgat ccgccgcgcc tggtcctcga
     2701 acctccgagg gaagggcacg aagtacgcct ccttgcggag gtaccgggct tcccagaact
     2761 ggttgtaccc ggccccgcag accaccaggt cgaaggcgtt gtaccagtcc atggccggga
     2821 agagcccgcc ctcccggttg tccgaccgca cgacggtcca gccctcttcc tcgaggtagg
     2881 agaagctctt ccatgcctcc gccccttccc cggtccgtcc gctgaaggcg aagaggcagg
     2941 cccggtcctc gggccccagc cctaggtccg cccgggcggc ctcagccggc aggatctcgt
     3001 ccctattacg gagcaccagg ggctccacgg actccgaggg gaagggcagg tcgaagcccg
     3061 gctcgctgcg caggacaagg tcccagtcct cgggccggaa gacgagttcc cggtccgggg
     3121 tccggaaccg gaaagtcctg ggatcgatct ggcgggtgag gaagactttc ttgcagggga
     3181 ggtcgcggag gaaggagtcc agggcgaacc agtggatatc ggccaccagg acgtcgggct
     3241 tggaggactc gatggctcgg aatagagcgg aatcgcggta ccgatcggga ccgagggcgg
     3301 tctcgtcctc aggaggaatc tgctctattg gcacaccgaa ggcttccgcg aatcggccat
     3361 actcggtgtg taccgagagg atcgagaagt cggcggccgc cccggaacga cggaaggcat
     3421 taacgagagt aaggccccgg gtaacatgcc cggagcccga gagcccgccg gtatagaaaa
     3481 gaacgcgcaa tgttggtcag cagaaatagg attcaaaaaa ggatactaca ccagtagtaa
     3541 cctgcaattt ggatacgaaa ccagtatcaa gatcgacttc aacaatacga tcaagattgg
     3601 atccgtcgaa tccttcatct gatacgatca gcttacgagg tgtaattgca agaaatcttg
     3661 aagggccata gaattggcta ctaggagaac ttggagtgcc tgatgaccag ccaatctcat
     3721 caatcatgct gaggtcagac aaggataatt tgagtatttt cccacgttga tttggtgtca
     3781 ggtgttctgc agcggcgata tacagatggc cgtccttgac cacggcatcc ttcggtagta
     3841 ttgaactaga caggcctagt tcgccgtagg tccgtcctac cagggactgc gcatccgaca
     3901 ttggcgcacc cgcgccgtat ataggcccca aggagtactt aaacacccag tcctggttgt
     3961 tgtaattagc gttcgagtat atataacctg ccggatcaac agcgataatt ctggaatccc
     4021 cggggcttga atgccctgcg ggcaaattaa taataacgct agaaccatca acgagatcgt
     4081 aatacgatgt ggaaagggag gctaaaaaaa ctcggtcatg gacattgtcg atggtgatac
     4141 gaccagggga ggtatagttc tctatccaaa atggagtact gctgggatca tccagccgaa
     4201 atactcttcc gacacctgac ccatcatttg ctgaaaaata gagccttcca aacttgtcaa
     4261 attcaaaatc ggaattctgt aatatgacat tccccgccag atctattgaa acgggatttt
     4321 gatctccgat gtccaaaccg taaagcaacg atgaataagc actaggcaac agtatcttcg
     4381 tctccaccac ccccatgtcc atggacaagt taaccttctg cccccccgcc acatccacca
     4441 ccgcggtccc gccgtaggcc ttggccaggg tcggcagggg atcaaatatg atatctgccg
     4501 tcgccgccca gtccggcacg gcatacacct ccaccttccg ggcggggccc gcgggcacct
     4561 ggatcgttag gctgcccgcg tttcccggca cggtctggga cgccgtgggc atgccggacc
     4621 cgctgacgct gacggtcacc gaggagaaga ccgggaggcc cgagaaaggc gcgtccgaga
     4681 aggcccggga gacggggccg cctcccaggt tcaggattat cgtcccctcg gcccggggcc
     4741 ccagggccag ctcgcaggag gccagggtca gtagagcggc ggccgccgcc gcaagggtcg
     4801 catatctcct tttcatccca tacctcctca gtcaatcggc gcgccgaccc agggattgaa
     4861 ccgcaggtcc acggttcccc aggaagcccc ggagttgtcc accaggccct gcacgaagga
     4921 gaggatcgat ccgtccaggc ccgcgggcgg gggcggggag agggcctggt ccggcatgtc
     4981 cttcgggggc acggcgcccc cgtcctcgcc gacggtgctg gactggcccg cgccgacctg
     5041 gacgggcagt ccgtcggggc cggtgaactc gacgttcccg tcgtccacgt ccaggttcac
     5101 cgggtcctgg tcgaagccgg tgccccggac ggaggcggtc gcggtggggc tcttcacggt
     5161 gaagtccgtc ttgccgcccg ccgggggctt cacctcggcc cggacccggc cggaaagcag
     5221 ggccaggcgg acggtttcgg aaccctccag ccgggcgatc tcctccaggg agaggcgggt
     5281 cagaggtttg accgtgaggg tggaggttcc gacgctgatc accgccacgc tcttgaaggc
     5341 cgtggagatc agggtgtcct tggtcagggc cgcgccggcc ctggccggga cccagtcccg
     5401 gccgggggcc ttgatctcca cggtccccgc cacctgccgg atcaccgcca ggggagccgc
     5461 cgcctgggcc gcggccagga cggcggcggc caggaggatc gaaatcgcca ggattcgttt
     5521 cattccattc tcctcgctca cagcgaaagg acggcggcca cggacaggag ccaccgggcc
     5581 ggggtgtccg gcaggaaggc ccctcccgag gcgggaagga agatccccgt acccgccagg
     5641 acggagaggt ccgacaaggg ggtccaggtc agggaggcgt agagctccgc ccccagccaa
     5701 gccgaatcgg aaccgggatc cagctcggcg tccgcggggc ctccctcggc gaagcggaga
     5761 aagccgaccg cctggacctc ggcgcccagg acggcggagg gccggacctt gtagccgagc
     5821 cgggcgctgg ccaggccgga gggtccgggc gtgaagaccc ggccctgctc cagggaggtg
     5881 atgttcggga agcctccgtc cgcgccggtg gccgcccgca ggcccaggta ggcctgatcc
     5941 ttcagggccc ccgggggatc ccacgcggct tgcagggcgc cggccgcgtg gaagaccggg
     6001 tccgagcccg aaggctcggc cagggcggcc gcggcgctcg cctcgaatcc gaaggcgtcc
     6061 ccggcgggga tgcccgcggt cagggtgagg tactgggagt ggagggtgtc cgatcccccc
     6121 cgcaggtcga actggccgac cgcctgggcg gacagccggg acaggaaggc agggggattc
     6181 cgcaggcccc cggagaggac cgccaggagc cggggagggg cgaagaagcg gtcggcatcc
     6241 aggaggtccg ccccgtccgc ggcggtcagg gcgatctccg cggacttctt gttcaggaga
     6301 cccgtataga agagcccggc ggaggcgccc agggagccca gggatccggt ccaccgcgcc
     6361 ccgtccagga gccccgaggc cacgaagccg gactcgtccg cgaagggcat gcggccgaac
     6421 tcccaggaga tcccggggga aggccgcagt ttgacctggg accgccccag gtccagaagg
     6481 agggttgccg attggaaggt ggtctccagc ttgacgatcc ccgagaagta gagctccccg
     6541 ttctctccca ggagggcgga gtaccaggga ttggccatga ccgtcaggga gaagccttcc
     6601 tgggaaacct tgggtgccag gctcagggcc gtgccgaagt ccgaggctcc tgcggaggcg
     6661 gccgggatca ggaggacggc cgcggcagcc agggggagga gggtccgacg ccgtatcatt
     6721 ccccgcctcc cttcgcgtcc aggacgcggc ccaggatccg gaccagccgt tcgcccggaa
     6781 ggccccgggc gggatcccgc cgcccctgga tgaactgtcg atagaccagt tcccggtagg
     6841 cgtagcgggg acccgggaag aggcggtaca tcagccctcc ttccagggag aaggcccgca
     6901 ggatcaggta ggcgctttcg tccaggcgga tcggggcggc gggatcggcc ccgtccggga
     6961 gccagccccg ttccagggcg gccttgtagg cgtccgcctc ggaaacggcg gcgggcagga
     7021 ttcccgcggc cggcaggatg tagcgggccg cccgggcgta gcttactccc ggatccgcca
     7081 gcaggaggtc catctccttg gcggactggg cgtacagcgc cgccgccgcg gccaggaaca
     7141 gcaccggaaa cgtttttctc atatccattc cttccttgaa tattcagaaa aaggaacagc
     7201 cgccgaatgt tgtatccctc aatataacct actatactgc ggatgtcctt gaaatcccaa
     7261 cgggatttcg ggatttttta ctagttcgct cagaaataga atcgttgacc cgcgccgttt
     7321 cccgaaggag gatttcgtga agaaccagcg cgctcccgcc ctcgcggcga tcggatcggc
     7381 cgttctcttc tcggccctct acctgaccag cttcttccgg agcgcggagt ccggggtcta
     7441 cgatctcttc ctgggcctca agcccccccg tccccgcacg gaccgggtcg ccctcctgga
     7501 cgtggacgac cgggccatag cccacgtggg ggtcttcccc tggccccggt ccgtggtggc
     7561 ggacggactg ttgcggctca aggagttcgg ggccgccgcc gtggtcttcg atatcgagta
     7621 cctggattcc agcccccccg ggatcgacgc cgtctacctc caacgcggcc taccccggga
     7681 tttccagcgc agcttcggag acatcggcac caacgtctcg gacctcttca acgccctgac
     7741 ctcgggccag atctcgcccc gggaggcccg gggctacgcg gcccagctgg cggacctcat
     7801 cgcctcggag cgggacgggc tcctggcccg ggccaggggc ctggcccagg acaacgaccg
     7861 ctacctcggg caggccgccc ggctcaacgg acgggtctgg gccacggtca acctccagac
     7921 ggtcaaactg gagggcgagc aggccgagcg gagggccggc gcccgggagc gcttttccgt
     7981 aacggtggaa gccgccccgg aggcccccgc gggggactac gtggacgtcc tggcccccat
     8041 cccctacttc ctggaagccg cccggggggc gggcttcacg aacgtggtga tcgacccgga
     8101 cggggtccgc aggcggatct ccctgacccg tcgggtggac ggggcctggt acctccagct
     8161 tgtcacggcc cccctcctgg aagccctggg gaacccgaag ctcgtcctgg agcggcggcg
     8221 gctgcgcctg ctgggcgcct ccctgcccga gggcgggacc ggggacatcg acatccccct
     8281 ggaccccgag ggccacatgc tgatcgactg gccccgggag aactacttcg agagctacga
     8341 ccacgtctcc ttctactccc tgtcgcggct ggaggagctg gaggccgctc tgggcgagtc
     8401 cgtctccgcc ctggcctccc tggaatcctg gttcctcccg gatcccgagg gagagctggt
     8461 cctggcctac gcgaccctgg cgggcctgac ggaaaccctg gacgcctcgg aggcctctct
     8521 ccggaccgcc ctggaaagcg cctcggcgga ggacttcgac cagtggcgcc tgggccggca
     8581 ggaggcccgc tcgggactca aggacctcct ggcctccgga gccgtggaca ccctgcgccg
     8641 gaccgcggag gcgacggccg cctcggcccg gcccgacgag gccgcggccc tcaaggcgga
     8701 ggccgagacg gcggggaagc tggcctcggg cctggcctcg atcctggcgg agatcgagga
     8761 gatccggacc cgcctggacg cggccttcgc cggaaaaata gtcatcatcg gacgggtgga
     8821 cacgggtacc acggatatcg gggtcaaccc cttccacggg gagtacatca acgtcggtac
     8881 ccacgcggcc gtggcggaca ccatcctggc ccgcaccttc atccggcccg tcgggtcctg
     8941 ggcctcgatc ctcctggccc tgctggcggc ccccctcttc gtcctgacca tgggccgctt
     9001 caaggccggg gtacggatcg ccgtgggcct gggcggcgcg gccctggtct tctggggttc
     9061 ctacgccatc ttcgcggcgg cccggatctt cgtgggcccc ctgggcgcaa ccctggcggt
     9121 catcctggcc tccgtggtcc gggaggccgt ggacttcgtg agcaccgagc gggagaagcg
     9181 cttccttagg aaggccttcg atacctacgt ctccccggaa tacgtgaacg agctcgtggc
     9241 ccagcccggc agcctcaagc tgggcggatc caagcgctgg atgaccgccc tgttcacgga
     9301 cgtccggggc ttctccacca tctcggaaca gctcgatccc gaggccctgg tgggcctcct
     9361 gaaccgctac ctttcgacca tgagcgacat catcctggag gagcggggaa ccatcgacaa
     9421 gtacgagggg gacgccatca tcgccttctt cggggcgccc ctgaccctgc aggaccacgc
     9481 ccggaggtcc ctccgctccg ccatcctcat gaagcgggcg gaagcccggc tgaacgaggc
     9541 ggtcctggcc gagggcctca cccccgcccc cctggccacc cgcatcggga tcaacaccgg
     9601 ggacatggtg gtgggcaaca tgggcaccga gaagaagatg aactacacca tgatggggaa
     9661 cgccgtgaac ctggccgccc ggctggaggg ggtgaacaaa cagttcaact cctggatcct
     9721 gaccaccgag gccacggtca aggaagcggg gcccgagttc ctggtgcgcc gcacggcccg
     9781 ggtccgggtg gtcgggatct tcgagcccgt ccggctctac gaggtcctgg acttcgtatc
     9841 cgaggctccg gaaaagagga agaccaccgt ggagctcttc caccgggccc tggaggtctt
     9901 cgaggccaag gactggaccg aggcgggcaa gctctttctc aaggtgcttt cccacgaccc
     9961 cgaggacgga ccgtcccagt cctacctggc gcgcctggac ggattccgga agaagccccc
    10021 ggccaaggac tgggacgggg tcttcaacct gacggaaaag tgacgtgagc agggaaggcg
    10081 aaaagggact gtgccgccgc atcctcgagg cggccaacga cggggtgatc gtcacggacc
    10141 cggacggcgc cattctcgag gccaaccccg ccttcctggc catgacgggc tacggaatgg
    10201 acgaggtggc cgggaagaac gcgcgcatcc tgaactccgg ccggcacgac gcggagttct
    10261 accagagcct ttgggccgag gtccggtccg cgggagcctg gaccggggag atctggaacc
    10321 gccgcaagag cggggagatc tatccctgcc gggtcaagat ccaggccgtc ccggacgagg
    10381 ccggcgaggc gtccggattc atcggaatat tctcggacct gtcgcccctc aaggactcgg
    10441 aggagcgcct gcggaacgcg gtcctcttcg accccctcac cggcctgccc aaccgctccc
    10501 tcttccggga ccgcctggcc cagatcctgg accgggcgcg gaagaaggcc acccgggcgg
    10561 ccctggtctt cgtggacctg gaccggttca agcacatcaa cgaaagcctg ggctaccagg
    10621 cgggggacga gctgctgcgg gaggcggcgc ggaggctcac ggagcgtacg ggcggggcgg
    10681 gcacggtctg ccgcctgggc ggggacgagt tcgcggtgat cctggatacc gtccaacgga
    10741 gcgaggacgc gggcgccgcg gccgcgggaa tcctggccgc cctgtccgcg ggcttcccca
    10801 tgggcccctc ggaggtccac accggggcca gcgcgggaat cgccgtcttc cccttcgacg
    10861 gcaccgacgc gggggccctg gagaaaaagg ccgaggccgc catgtacgaa gccaaagaag
    10921 caggcagggg tcggtaccgc ttcgcctccg gcaaggtcgg gcataccagc cggcgccagc
    10981 tggagctgga gtcccgactg cgccgcgccc tggagcgggg cgagttctac ctgacctacc
    11041 agccccaggc cgccacgggc ggggtcgtac ccggcacggg ggcgggcctc atgggggcgg
    11101 aggccctgat ccgctggaag cccgaggggg ccgataccgt cagccccggg gacttcatgg
    11161 acatcgcgga atccacggga ctcatcgtgc ccatggggga atgggccctc aaaaccgcct
    11221 gcgcggacgc caagctctgg aacgacctcg gccggcccct gacagtgtcc gtcaacgtat
    11281 ccccccggca gttccaggcg gggaccctgg cccgggtgac ccgggaggcc ctggacgcct
    11341 cgggcctcga cccgggacgg ctcaagctgg agatcaccga gaccctgttg atggaccaca
    11401 tgggcgagac cgtcacggtg atgaacgaga tctacccgca gccgagcctg ccggagaacg
    11461 ccgccgaggg catcgtccgc gggctctacc gctgcggcgg gcgcggcgac cccgacgcgc
    11521 cggtgcgggt gcggctgctc ggctcgggca cgatcctgcc acaggtcatc gccgccgccg
    11581 atctgctggc cgagcggtac ggcgtcgcct ccgacgtcct
//
LOCUS       DAOTEI010000108        11527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3937_length_11527_cov_5.068863, whole genome shotgun sequence.
ACCESSION   DAOTEI010000108 DAOTEI010000000
VERSION     DAOTEI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_11527_cov_5.068863"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..627)
                     /locus_tag="P5313_10870"
     CDS             complement(97..627)
                     /locus_tag="P5313_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80908.1"
                     /translation="MDEQYRDVPGRDGARVLMGVSFSAKVTKDGATLALKRTAKGVSG
                     PSIADRAAHTMVHEVLPDVFYGNLYGWKKTLRPGMPLMDTGTHLVSGFVYKVAGSVAT
                     IKNLFRYAHIHDKGATIRAKNKPLLKFKPRGLGWMSKKQVVIPARRFMYWSALAKAAV
                     MRSVNSMIRTAVRGQK"
     gene            complement(576..1031)
                     /locus_tag="P5313_10875"
     CDS             complement(576..1031)
                     /locus_tag="P5313_10875"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HRY80909.1"
                     /translation="MPNLYAAAADVLATLSAAILDDLAKDTDGGTDHTLTLIESMSRR
                     ADGLIRQHKPSGYIVPLTDTQAIETVRPWIVDVTRFMLLERALAGKYDTEGAKFLYEK
                     ALAYFESVAKGEMDIPGAVDYGASASTAAGTTSWTSNTVMYPVGTARGF"
     gene            complement(1031..1660)
                     /locus_tag="P5313_10880"
     CDS             complement(1031..1660)
                     /locus_tag="P5313_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HRY80910.1"
                     /translation="MSDFPLLDRTLALWLKRGLITPEQAASIADVLRGQVFSLAGEWR
                     EKVLEKVRTMLEKAMAEGWSVKEFKDAASQTLARFTDGNYAEIVFRTNVANARAAGRY
                     EEMFSPEYAEVAPYWEFRAITDARNDEEDECPDKRCRWLNGKTFRKDDPAAQQYLPPL
                     HFQCRCMSVERTEGSIRSEVISGDAVPFSPVAGFGGTTLMSFLNLFGGK"
     gene            complement(1657..1890)
                     /locus_tag="P5313_10885"
     CDS             complement(1657..1890)
                     /locus_tag="P5313_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRY80911.1"
                     /translation="MGAVAREYGFKGVFFPELTDPETGLEYACRLLANLLSRYSVEDA
                     VSAYNAGSPTQRNERDYVQPVLRWAAGYKAVGL"
     gene            complement(1885..2028)
                     /locus_tag="P5313_10890"
                     /pseudo
     CDS             complement(1885..2028)
                     /locus_tag="P5313_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
     gene            complement(2040..2246)
                     /locus_tag="P5313_10895"
     CDS             complement(2040..2246)
                     /locus_tag="P5313_10895"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HRY80912.1"
                     /translation="MDAGDRFEALEIKAAYQEESLAKLNGEVWDLSRRMDRLEDLVTE
                     LRRKLAEAEGSEPGAPADRRPPHY"
     gene            2407..4362
                     /locus_tag="P5313_10900"
     CDS             2407..4362
                     /locus_tag="P5313_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY80913.1"
                     /translation="MAKRTSLLVKFSAVISLMVALMLGAVILVVGLRLSADLTEITRN
                     SSLEIVESHAAEIGQLFRGLLYQLSQIAARPELRSGEPASALEATKAGAAAASEEANM
                     ALYAERDGSYVTSAGGSGNITTRAYFQGVFVQGRDFAFGDPVISQTTGLPTVHLAKAV
                     KGPDGRTVRLVAYQLKLESLSAIAVSIKVGRTGFGWIVTQTGLLLAHPDPKAVMNLNL
                     TEADKAGYRGMDALAKRLLAEESGVGAYLDPAGVPMTAYFVRIPETPGWVLGVSLPTA
                     EVAEAAAPILRVLLILLFVSIAAAVLVSVLVTRTITRPIRGTAGIMVRLAAGDLALAG
                     ADADTARRLAARTDELGDLGRSMTELKDKLVEVAAGMRAASDEVAGGSSQLSQTAQTL
                     SQGTTEQAASIEELSASVEQLAATVKQNADNTAQADALARRVAQSADSSGKAVQETVA
                     SMGEIASRVSVIEEIARQTNLLALNAAIEAARAGEAGKGFAVVATEVRKLAENSQKAA
                     AQINDLSRSSMAVAEEAGKLLAELVPDIRKAADLIQEIAAASSEQAGGAEQISKGVSQ
                     MDTVVEQNAAASEELASTAEELNAQATLLADRVSFFRLSAEGSGPAIGRVRAPAPAAA
                     APGRSTALALPKKAPAADGEDGDFEEF"
     gene            4451..5980
                     /locus_tag="P5313_10905"
     CDS             4451..5980
                     /locus_tag="P5313_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRY80914.1"
                     /translation="MRILRAFRFPRRAVRAAALALLVFAAAAGAAAEGSSVYVIPIKG
                     DIEPSTAVFVRRNAEAALRAGAKILVFDIDTFGGRVDSALRISSYIGSIRGARTVAFV
                     SSGPESMGVSWSAGALIAFSASAIYMAPGTSMGAAAPVIMSPDGQSQGAGEKTVSAVR
                     SQMAALAEKNGHPPLLALAMVDADVELIELSVAGETRLATAEETASLEKSRPGQVERV
                     RTVSAKGKLLSLTAGEAERYGLSEGTVADLDALASLLGSPGPAVEVSPSGADQVVVFI
                     TSGGVQALLILIGLVALFLEINSPGFGLPGTVAIIAFFTLFGTNALMGSVGSLEIILF
                     ILGIGLLAVEIFILPGFGVAGISGIVLIGAALVFSMQDFVIPTVSWEWDLLGRNVMTV
                     VAGLLAGIAGIGVLALAGPRIKLFDRLMLKTTIEGTAGGALGADAEAPAPPASPLEGR
                     RGVARTVLRPSGRAEIDGVAYSVETEGLFLPEGTPVRVLKVRGSRIVVGPDAGNSDIE
                     G"
     gene            6026..7015
                     /gene="floA"
                     /locus_tag="P5313_10910"
     CDS             6026..7015
                     /gene="floA"
                     /locus_tag="P5313_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527462.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRY80915.1"
                     /translation="MLIIALYAVIALVSLGILVLFFKFFGLWFRALLSGAAVGMGRLV
                     GMWLRHVNPAVIVDARIMLTKAGIKVDSDMLETHFLSRGNVMKVSLALVAANKADIPL
                     PFQRAAAIDLAGRDVLEAVKTSVNPKVIDCPDTTKGKTTIDAVAKDGIQLQVKARVTV
                     RANIERLVGGATEETIIARVGEGIVTTIGSSESYKSVLENPDTISKRVLEKGLDAGTA
                     FEILSIDIADIDVGANVGAKLQADQAEADKRRFQAEAEKRRAAAQAQEQEMIAKVQEN
                     RAKVVLAEAEIPLAIAEAFRTGKLGVMDYYRMTNIQADTQMRKSIGGEEAGRK"
     gene            7022..7678
                     /locus_tag="P5313_10915"
     CDS             7022..7678
                     /locus_tag="P5313_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80916.1"
                     /translation="MDDDLFSTIIYMVPVALVIFLRVLGGGRRAQAAKERRAKEDAAR
                     RELARRVQADLEASGRAAPLSRISGREPESQEWEPHWVTESDSDLAEAAEARPGLEIS
                     DEEGPEAGQRLAFLETETGLRPPALEARDLSAFAPSLAAETVPVPATAAPGRFDAYPI
                     SGAGPALPSPADVSAAGAAASREAAQADSVPFTGKVERLPPLKRAVILSEILGPPKGA
                     "
     gene            complement(7776..8606)
                     /locus_tag="P5313_10920"
     CDS             complement(7776..8606)
                     /locus_tag="P5313_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80917.1"
                     /translation="MFGSLDTYSCLDQRDRREFSYYQDLYEDALLAIDDRIMARALEP
                     VDLQEYPQVLSFPRYDIRAALFIGSFDPFQMTHLAAALRYLASPESTAPLVFIIPEGH
                     VNPDKPRRSEYTYRSQMIRWQIEGVFQPLIYPLDIGEGADTIEIVRRFIARFPGARVH
                     VTHLVGSDVLPYALKWLPKDMEVWGAEAKYQGVTFQYDIFAIQRGGDEGWRKAAEDAW
                     SKGVTVRYDERSIGTPSSTDFRSNQAFSIVFPTKAVMRHVEVLFRYDLNKPWDPPTTP
                     "
     gene            8812..9741
                     /locus_tag="P5313_10925"
     CDS             8812..9741
                     /locus_tag="P5313_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY80918.1"
                     /translation="MRRVLAIDMGGTKTAAGVVDEAGRVLSKRTVPTPRENPRAAVEA
                     MAALSRELLSEAGPVESAGLALPGIVDRARGVLVRSASSGWTEVPFQAMVSEALGLPV
                     ASENDVNACALAEARFGGGAGMDCFFWMTISTGIGGAVYSGGRIVGGPMSGEVGHLVV
                     NPGGTPCGCGNRGCLEAEAAGPAWRRRALELMGGVPDAQADAAARVVDARTVAGGART
                     GDPLCLRVVEDCARPLAAGLAAVFNLLDPDAVFLGGGVAEAFDLLAPILRRELPPLVL
                     LGEDRPLPVLKSSLGYDAALVGAAVPALFPYSS"
     gene            9754..10353
                     /locus_tag="P5313_10930"
     CDS             9754..10353
                     /locus_tag="P5313_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY80919.1"
                     /translation="MKRDFTGPLEAYIDRLKSTLDKISREEINGFLNLLVDALEAGRN
                     VFTLGNGGSAATASHMAVDFNKGLSWGKARRFRFLCLSDNLPTLTAYANDVSYEDVFV
                     EPLKNFLESGDLVIAISGSGNSRNVVKAVEYANAHGAVTVGLTGYDGGLLRKTARHGV
                     HVPIRDMQVTEDLHMVLDHLAYSVLGAYLPDEDSGANHG"
     gene            10346..11344
                     /locus_tag="P5313_10935"
     CDS             10346..11344
                     /locus_tag="P5313_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY80920.1"
                     /translation="MAERDYRAVGPLPSSTRFLAGTSIEVAREIPRGPAPEHALFDFD
                     GTLSLIREGWMDVMVPLLAGILSEYARPGETEADISALVKDFVTELTGKQTIYQMIRL
                     AEEIRARGGVPRDPRDYKAEYHERLMERIASRRKGLADGTLPRAEALVPGSLEILGLL
                     RDRGVRLYIASGTDEEYVLEEAALLGVDEFAPGRIYGAQADYASFSKEMVIRRILAEN
                     RVDGRRLAAFGDGYVEIADCKAAGGVAVAVASDESGRSGRPDAWKRERLLGAGADLVV
                     PDYRDAKALVAYVWDGIEPESAADAAPAGRAEPRNGAAADAAGARPSGGPGRGGPR"
     gene            11341..>11527
                     /locus_tag="P5313_10940"
     CDS             11341..>11527
                     /locus_tag="P5313_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80921.1"
                     /translation="MIPGLERFGPERLSELAARFGSLRVAVLGDFFLDKYYEVDPARA
                     EPSLETGRTAHQVVGVRH"
BASE COUNT         1760 a         3930 c         4053 g         1784 t
ORIGIN      
        1 gctggtagga aagcagcgtc cgctgggcca cttgctgcgt cgcggtgacg gcggtgatgg
       61 cagccttgat gccggtgcgc atggtgttga cggtgctcac ttctgccccc gaaccgccgt
      121 gcggatcatc gagttcacgc tccgcattac cgccgccttc gccaaggccg accagtacat
      181 gaaccggcgg gcggggatga cgacctgctt cttcgacatc cagccgagcc cgcgcggctt
      241 gaacttcaga agcggcttgt tcttggcccg gatcgtcgcg cccttgtcat ggatgtgcgc
      301 gtaccggaag aggttcttga tcgtcgcgac ggagcccgcg accttgtaaa cgaacccgga
      361 gacgaggtgc gtcccggtgt ccatgagcgg catccccggg cgcagcgtct tcttccagcc
      421 gtagaggttc ccgtagaaca cgtcggggag cacctcgtgg accatcgtgt gcgccgccct
      481 gtcggcgatg ctcggcccgg agacgccctt cgcagtccgc ttcagcgcga gcgtcgcgcc
      541 gtccttcgtg accttcgccg agaacgagac gcccatcaga accctcgcgc cgtcccgacc
      601 gggtacatca cggtattgct cgtccacgaa gtcgtcccgg ctgccgtgga ggcggacgca
      661 ccgtaatcga cggcccccgg gatgtccatc tcgcctttcg cgacgctctc gaaataggcg
      721 agtgcctttt cgtagaggaa cttcgcccct tcggtgtcgt acttcccggc cagcgcccgc
      781 tcgagcagca tgaaccgcgt cacgtcgacg atccacggac gcaccgtctc gatggcctgc
      841 gtatccgtca acggaacgat gtacccgctc ggcttgtgtt gcctgataag tccgtcagcg
      901 cgccgcgaca tcgactcgat gagcgtcagc gtgtgatccg tcccgccgtc cgtgtccttc
      961 gcgagatcgt cgagaatcgc cgcgctaagc gtagcgagca cgtcggccgc ggccgcgtag
     1021 agattcggca tcacttccct ccgaacaggt tgaggaacga catcagcgtg gtaccgccga
     1081 aacccgcgac cggcgagaac gggacggcgt cgcccgagat gacctcgcta cgaatcgagc
     1141 cctcagtccg ctcgacgctc atgcaacggc actggaagtg cagcggcggc aggtactgct
     1201 gcgctgccgg gtcatccttg cggaacgtct tcccgttcag ccagcggcac cgcttgtcgg
     1261 ggcactcgtc ctcttcgtcg ttccgcgcgt ccgtgatggc ccggaactcc cagtacgggg
     1321 cgacctcggc gtactccggc gagaacatct cctcgtagcg cccggccgcc cgcgcgttcg
     1381 cgacgttcgt ccggaagacg atctcggcgt agttcccgtc cgtgaaccgg gccagcgtct
     1441 gcgaggcggc gtccttgaac tccttgactg accagccctc cgccatcgcc ttctcgagca
     1501 tcgtccggac cttctcgagc accttctcgc gccactcgcc ggccagcgag aacacctgcc
     1561 cgcgcagtac gtccgcgatg ctcgcggcct gctccggcgt gatgagcccg cgcttcagcc
     1621 agagcgccag cgtccggtcc agcagcggga agtcgctcac agcccgaccg ccttgtaccc
     1681 ggccgcccag cgcaggacgg gctggacgta gtcacgctcg ttccgctgcg tcgggctccc
     1741 ggcgttgtag gcgctcaccg cgtcctcgac ggagtaccgc gagagcaggt tggcgaggag
     1801 gcggcaggcg tattcgaggc cagtctccgg atcggtcagc tcggggaaga acacgccttt
     1861 gaatccgtac tcccgggcga cggcgcccat gaccccggaa tccccgcccc cgtagaccag
     1921 gcgcaggccg cggcgcgcga tctcccgacc gaaggctccg gccgcctcga ggtacgccgg
     1981 ggaggagccc gaactggcgc cgcagaagac gcagaccgac ttcagcatgc cgacctccct
     2041 caatagtgcg gcgggcgacg gtccgcgggg gccccgggct cggatccttc ggcctcggcc
     2101 agcttgcgcc ggagctcggt caccaggtct tcaaggcgat ccatgcggcg ggagaggtcc
     2161 cagacctcgc cgttgagctt ggcgagggat tcctcctgat acgcggcctt gatctccaga
     2221 gcctcgaagc gatctcccgc gtccatcggc cttcctcccg cggcccggcg tgccgggacc
     2281 gcagggctac ggtaaaccgg ggaagggcgc cttggctagc cgaggctcgc ggcccgccgt
     2341 aattgcatct tccgtcggat ggattagact gaagtaagcg agatccgcga cccgggaggt
     2401 ccgaagatgg caaagagaac ttccttgttg gtcaagtttt ccgcggtcat cagcctgatg
     2461 gtggccctga tgctgggggc ggtgatcctg gtggtcggcc tgcggctttc ggcggacctg
     2521 acggagatca cccggaactc gagcctggag atcgtggaat cccacgccgc ggagatcggc
     2581 cagcttttcc gagggctcct ctaccagctg agccagatcg ccgcccgccc ggaactccgg
     2641 tccggcgagc cggcttcagc cctggaagcg accaaggccg gggccgccgc ggcctcggag
     2701 gaggcgaaca tggccctcta cgcggagcgg gacggctcgt acgtcaccag cgcgggaggg
     2761 agcggcaaca tcacgacccg ggcctacttc cagggcgtct tcgtccaggg ccgggatttc
     2821 gccttcgggg acccggtgat ctcccagacc acgggactgc ccacggtgca cctggccaag
     2881 gccgtgaagg gccccgacgg ccgtacggtc cggctggtgg cctaccagct caagctggaa
     2941 tccctgtccg ccatcgcggt ctcgatcaag gtggggcgaa cgggcttcgg gtggatcgtg
     3001 acccagacgg gcctgcttct ggcccacccg gatccgaagg cggtgatgaa cctgaacctc
     3061 accgaggcgg acaaggcggg ctaccggggc atggacgccc tggccaagcg cctcctggcg
     3121 gaggagtccg gggtcggggc ctacctcgat cccgcgggag tccccatgac cgcctacttc
     3181 gtccgcatcc ccgagacccc cggctgggtc ctgggggtca gccttcccac cgcggaggtg
     3241 gccgaggccg cggcccccat tctgcgggta ctcctgatcc tgctgtttgt atccatagcc
     3301 gcggcggtcc tggtctccgt cctggtgacc cggaccatca cccggccgat ccgggggacg
     3361 gcggggatca tggtccgcct ggccgccgga gacctggccc tggccggcgc ggacgcggat
     3421 accgcgcgcc gcctggccgc ccggacggac gagctcgggg acctggggcg gtccatgacg
     3481 gagctcaagg acaagctcgt ggaggtggcg gcggggatga gggccgcctc ggacgaggtg
     3541 gccgggggct cctcccagct ctcccagacc gcccagaccc tgtcccaggg gaccacggag
     3601 caggcggcca gcatcgagga actctccgcc tcggtggaac agctggcggc cacggtcaag
     3661 cagaacgcgg acaacaccgc ccaggcggac gccctggccc gccgggtggc ccagagcgcg
     3721 gactcctcgg gcaaggccgt gcaggagacc gtggccagca tgggcgaaat cgcctcccgg
     3781 gtgtccgtga tcgaggagat cgcccgccag accaacctcc tggccctgaa cgcagccatc
     3841 gaggcggccc gcgccgggga ggccgggaag ggcttcgcgg tggtggccac ggaggtgcgc
     3901 aagctggcgg agaacagcca gaaggcggcg gcccagatca acgacctgtc ccggtccagc
     3961 atggccgtgg cggaggaggc ggggaaattg cttgccgaac tggtgcccga catccgcaag
     4021 gccgcggacc tcatccagga gatcgccgcg gcgtcctcgg agcaggcggg cggggcggag
     4081 cagatctcca agggcgtcag ccagatggac acggtggtgg agcagaacgc ggccgcctcg
     4141 gaggagctgg cgagcaccgc ggaggagctc aacgcccagg ccaccctcct tgcggatcgg
     4201 gtctccttct tccggctctc ggccgagggt tccgggccgg ccatagggcg cgtccgggcc
     4261 ccggcaccgg ccgccgcggc gcccggacgc tcgacggccc tagccctgcc caagaaggcc
     4321 cccgcggcgg acggggagga cggggacttc gaggaattct gaggaatccc ttccgacccg
     4381 ggccgattct tggtacctta taacccatgg acaggccggc cgacggggcc ggggttcggg
     4441 aggtggttcc ttgcgcatcc ttcgggcgtt ccgatttccg aggcgggcgg tccgcgccgc
     4501 ggccctcgcc ctgctcgttt tcgcggccgc ggcgggcgcc gcggccgagg gctcctcggt
     4561 ctacgtcatc cccatcaagg gcgacatcga gccctccacg gcggtcttcg tacggcgcaa
     4621 cgccgaagcg gccctgcgcg ccggggccaa gatactggtc ttcgacatcg acaccttcgg
     4681 gggccgggtg gactccgccc tccggatctc ctcctacatc ggctccatcc ggggagcccg
     4741 gaccgtggcc ttcgtgagct ccggccccga aagcatgggg gtgtcctggt ccgcgggggc
     4801 cctgatagcc ttctccgctt ccgccatcta tatggccccg gggaccagca tgggggccgc
     4861 ggcgcccgtg atcatgagcc cagacggcca gagccagggc gccggggaga agaccgtcag
     4921 cgcggtccgg tcccagatgg cggctctggc ggagaagaac ggacacccgc ccctcctggc
     4981 cctggccatg gtggacgcgg acgtggagct gatcgagctc tccgtggcgg gggagacgcg
     5041 cctggccacg gcggaggaga ccgcttccct ggagaagtcc cggcccggcc aggtggagcg
     5101 ggtgcggacg gtctccgcca agggcaagct cctttcgctg acggccgggg aggcggagcg
     5161 ctacggcctc tccgagggca ccgtagcgga cctggacgcc ctggcctccc tcctcggatc
     5221 gccggggccc gccgtggagg tgtccccttc cggggcggac caggtcgtgg tgttcatcac
     5281 ctccggggga gtccaagccc tgctcatcct gatagggctg gtggccctct tcctggagat
     5341 caactccccg ggcttcgggc ttccgggcac cgtggccatc atcgccttct tcacgctctt
     5401 cgggaccaac gccctgatgg gctcggtcgg ctccctggag atcatcctct tcatcctggg
     5461 gatcggcctt ctggcggtgg aaatcttcat cctgcccggc ttcggggtgg cggggatctc
     5521 cggcatcgtc ctgatcgggg cggccctggt cttctccatg caggacttcg tgatccccac
     5581 ggtgtcctgg gaatgggacc tcctgggccg gaacgtgatg accgtggtgg cgggcctcct
     5641 ggcggggatc gcggggatcg gggtcctggc cctggcgggc ccgaggatca agctcttcga
     5701 ccgcctgatg ctcaagacca ccatcgaagg gaccgcgggc ggcgccctcg gtgcggacgc
     5761 ggaggctccg gctccgcccg ccagccccct ggagggccgg cggggggtgg cacgcacggt
     5821 cctcaggcct tcgggccggg cggagatcga cggggtcgcc tacagcgtgg agacggaggg
     5881 cctcttcctg cccgagggaa caccggtccg ggtcctcaag gtccggggtt cccggatcgt
     5941 cgtcgggccg gatgcgggga attcggatat cgagggctga caagcccgaa aggatgatag
     6001 aatgtcggaa ttgttaggag gaagcatgct catcatagcg ctctacgcgg tcatcgccct
     6061 cgtctccctg gggatactcg tcctgttctt caagttcttc ggcctctggt tccgggccct
     6121 cctgtccgga gccgccgtgg gcatggggcg cctggtgggg atgtggctga ggcacgtcaa
     6181 ccccgcggtc atcgtggacg cccggatcat gctgaccaag gcgggcatca aggtggattc
     6241 ggacatgctg gagacccact tcctgtcccg gggcaacgtg atgaaggtca gcctggccct
     6301 cgtggcggcc aacaaggccg acatccccct gcccttccag cgagcggcgg ccatcgacct
     6361 ggcgggccgg gacgtcctgg aggcggtcaa gaccagcgtg aatcccaagg tcatcgactg
     6421 cccggacacg accaagggca agacgaccat cgacgcggtg gccaaggacg ggatccagct
     6481 ccaggtcaag gcccgggtaa cggtgcgggc caacatcgag cgcctggtgg gcggcgccac
     6541 ggaagagacc atcattgccc gggtgggcga gggcatcgtc accaccatcg ggtcctcgga
     6601 gtcctacaag tccgtgctgg agaacccgga caccatctcc aagcgggtcc tggagaaggg
     6661 cctggacgcg ggcaccgcct tcgagatcct gtccatcgac atcgcggaca tcgacgtggg
     6721 cgccaacgtg ggagccaagc tccaggcgga ccaggccgag gcggacaagc gccgcttcca
     6781 ggcggaggcc gagaagcgcc gcgccgcggc ccaggcccag gagcaggaga tgatcgcaaa
     6841 ggtccaggaa aaccgcgcca aggtggtcct ggcggaggcg gagatccccc tggccatcgc
     6901 ggaggccttc cggaccggca agctcggcgt catggactac taccgcatga ccaacatcca
     6961 ggcggacacc cagatgcgaa aatccatcgg cggcgaggaa gcgggcagga agtaggccgg
     7021 catggacgac gacctgttct ccacgatcat ctacatggtt cccgtcgcgc tggtcatctt
     7081 cctccgggtc ctcggaggag gacggcgggc ccaggccgcc aaggagcgga gggccaagga
     7141 ggatgccgcg cgccgggaac tggctcgaag ggtccaggcg gatctggaag cttcgggccg
     7201 cgccgctccc ctgtcccgga tctccggccg agagccggaa tcccaggaat gggagccaca
     7261 ctgggtgacc gagtcggatt cggacctggc ggaggcggcc gaggcgcgtc cgggtctcga
     7321 gatttcggac gaggaaggcc ccgaagccgg ccagcggctg gccttcctgg agaccgagac
     7381 cggccttcga cctcccgcac tcgaggcccg ggacctctcc gctttcgccc cgtccctggc
     7441 cgcggaaacc gtaccggttc cggcgacggc cgcccccggc cgcttcgatg cgtatccgat
     7501 ctcgggggcc ggacccgccc tgccttcccc cgcggacgta tccgccgccg gggccgcggc
     7561 atcgcgggaa gcggcgcagg cggattccgt gcccttcacc ggcaaggtcg aacgcctgcc
     7621 gcccctgaag agggccgtga tcctgtcgga aatcctgggt ccgccgaaag gggcgtgacc
     7681 gcggctatag ccgcggtcac ggcgccgaag cccgcagggc ttcgtcgatt ccatggaacc
     7741 ggcctaaagt gaagtgcccg gcggctgcgc cgccgtcacg gcgtcgtggg cgggtcccag
     7801 ggcttgttga ggtcgtagcg gaagagcacc tccacgtggc gcatcaccgc cttggtgggg
     7861 aagacgatgg aaaaggcctg gttgctccgg aagtccgtgc tggagggtgt gccgatgcta
     7921 cgctcgtcgt aacggaccgt cacgcccttg gaccaggcgt cctcggcggc cttcctccag
     7981 ccctcgtccc cgccccgctg gatggcgaag atgtcatact ggaaggtcac cccctggtac
     8041 ttcgcctccg ccccccagac ctccatgtcc ttgggcagcc acttcaacgc gtagggcagg
     8101 acgtcggagc ccaccagatg ggtgacatgg acccgggctc ccgggaaccg ggcgatgaaa
     8161 cgtcggacga tctcgatggt gtcggccccc tcgccgatgt ccaggggata gatgaggggc
     8221 tggaaaaccc cctcgatctg ccagcggatc atctgggagc ggtaggtgta ctcgctgcgc
     8281 cggggtttgt ccggattgac gtggccctcc gggatgatga agaccagggg ggcggtcgat
     8341 tccggggagg ccaaatagcg gagcgccgcg gccaggtggg tcatctggaa gggatcgaag
     8401 gagccgatga acagggccgc ccggatatcg tagcggggaa aggacaggac ctgggggtac
     8461 tcctggaggt ccacgggctc cagggcccgg gccatgatcc ggtcgtcgat ggccaggagg
     8521 gcatcctcgt agaggtcctg gtagtaagaa aactcccgcc ggtcccgttg gtccaggcag
     8581 gagtaggtgt cgaggcttcc gaacacactc ctcaccttgc cgagaatccg gcggatttcc
     8641 gaggagcggg gagtccgggg cggtcctgcg cgcgggggct cggtacgttc ggtcatcaat
     8701 cgatattgta gggtctattt ccgttccgcg caacgtgaaa tatcacgcga ggcgcgcagc
     8761 gcctcgcgcc ccctgcttgc ctctccccct cactccgggc agaatgtacc catgcggcgg
     8821 gttctggcga tcgacatggg aggtaccaag acggcggcgg gggtcgtgga cgaggcgggt
     8881 cgcgtcctgt ccaagcggac cgtccccacc ccccgggaaa acccccgggc cgcggtggag
     8941 gcgatggccg ccctctcccg ggaacttctg tccgaagccg ggcccgtgga gtccgcgggt
     9001 ctggccttgc cggggatcgt ggaccgcgcc cggggagtcc tggtccgctc ggcctcctcg
     9061 ggctggacgg aggtgccctt ccaggcgatg gtttcggaag ccctgggact gcccgtggcc
     9121 tccgagaacg acgtgaacgc ctgcgccttg gcggaagccc ggttcggcgg cggggccggg
     9181 atggactgct tcttctggat gacgatctcc acgggcatcg ggggtgccgt atactcggga
     9241 ggccggatcg tgggaggacc gatgtccggg gaggtcggcc acctggtcgt gaatcccggg
     9301 ggtaccccct gcggctgcgg gaaccggggc tgcctggagg ccgaggccgc cggccccgcc
     9361 tggcggcgcc gcgccctgga actcatgggc ggggtcccgg acgcgcaggc ggacgcggcg
     9421 gcgagggtgg tggacgcccg gaccgtggcc gggggcgccc gcaccgggga tccgctctgc
     9481 ctgcgggtgg tcgaggactg cgcccgcccc ctggcggcgg ggctggccgc ggtgttcaac
     9541 ctcctggacc cggacgcggt cttcctgggg ggcggcgtgg cggaggcctt cgacctcctc
     9601 gcgccgatcc tccgacggga gcttccgccc ctcgtactgc tcggcgagga ccggcccctg
     9661 cccgtgctga agagttccct gggctacgac gccgccctgg tgggcgccgc cgtgccggcc
     9721 ctgttcccct attcatcgta aggaaggaca attgtgaaac gagacttcac aggccccctg
     9781 gaagcgtaca tcgatcggct caagtcgaca ctggacaaga tctcccgcga ggagatcaac
     9841 ggcttcctca acctcctggt ggacgccctg gaggcgggac ggaacgtctt caccctgggc
     9901 aacgggggct ccgcggccac ggcctcccac atggccgtgg acttcaacaa gggcctgtcc
     9961 tgggggaagg cccggcgctt ccgcttcctt tgtctgtccg acaaccttcc cacccttacg
    10021 gcctacgcca acgacgtgtc ctacgaggac gtcttcgtgg agcccctcaa gaacttcctg
    10081 gagagcgggg acctggtgat cgcgatctcg gggtccggca attcccggaa cgtggtcaag
    10141 gcggtggagt acgccaacgc gcacggggcc gtgaccgtgg ggctcacggg ctacgacggg
    10201 ggccttttaa ggaagaccgc ccggcacggg gtccacgtcc cgatccggga catgcaggtc
    10261 accgaggatc tccacatggt cctggaccac ctggcctact cggtcctggg ggcgtacctc
    10321 cccgacgagg actccggggc gaaccatggc tgaacgcgac tacagggccg tcggccccct
    10381 cccctcgtcc acccgcttcc tggccggcac ctccatcgag gtggcccggg agatcccccg
    10441 gggccccgcg ccggagcacg ccctcttcga cttcgacggc accctgagcc tcatccggga
    10501 gggctggatg gacgtgatgg tccccctcct ggcggggatc ctctcggagt acgcccggcc
    10561 cggggagacc gaggcggaca tctcggcgct ggtgaaggac ttcgtgacgg aattgacggg
    10621 aaagcagacc atctaccaga tgatccgcct ggccgaggag atccgggccc gggggggcgt
    10681 cccgagggat ccccgggact acaaggccga gtatcacgaa cgcctcatgg aacggatcgc
    10741 gtcccggcgg aagggccttg cggacgggac cctgccccgg gcggaggccc tggtgcccgg
    10801 gtccctggag atcctgggcc tcctacggga ccggggtgtc cgcctctata tcgcctcggg
    10861 caccgacgag gagtacgtcc tggaagaggc cgccctactg ggggtggacg agttcgcccc
    10921 gggccggatc tacggggccc aggcggacta cgcgtccttc tccaaggaga tggtcatccg
    10981 gcgcatcctc gccgagaacc gggtggacgg ccgccgcctg gccgccttcg gggacgggta
    11041 cgtggagatc gcggactgca aggccgcggg gggtgtagcc gtggcggtgg ccagcgacga
    11101 gtccggccgc tcgggacggc cggacgcctg gaaacgggag cgcctcctgg gcgccggtgc
    11161 ggacctggtc gtgcccgact accgggacgc gaaagccctg gtcgcctacg tctgggacgg
    11221 gatcgagccg gaatccgccg cggacgccgc gccggccggc cgggccgagc ctcggaacgg
    11281 agccgccgcg gacgccgccg gcgcccggcc ttccgggggc cccggccggg gaggcccgcg
    11341 ttgatccccg ggctagagcg cttcggtccg gagcgccttt cggagctcgc agcccgcttc
    11401 ggctcgctcc gggtcgccgt gctgggggac ttcttcctgg acaagtacta cgaggtggac
    11461 cccgcccggg cggagccctc cctggagacg ggccgtacgg cccaccaggt ggtcggggtc
    11521 cgacatt
//
LOCUS       DAOTEI010000109        11502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3947_length_11502_cov_5.083603, whole genome shotgun sequence.
ACCESSION   DAOTEI010000109 DAOTEI010000000
VERSION     DAOTEI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11502
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3947_length_11502_cov_5.083603"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /gene="fliF"
                     /locus_tag="P5313_10945"
     CDS             complement(<1..1627)
                     /gene="fliF"
                     /locus_tag="P5313_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRY80922.1"
                     /translation="MNEWMKKFFEQLKTLWSKWTLVQKLILAGIVLAAVAGTVLLFRV
                     SSAPTMVRLISTPIRSDDELRLITSRLDEERIPFEVGEGNVLYVKDEKTRAQANAVLL
                     REDLIPKGTDPWSVFAIDRFTVTDFERNVNLRRAVVQEVRRHIESLGDVDKANVVIEI
                     PEKELFTEDQNPVTASVILYPKPGSDITANRKKLEGVQKIIQFAVGGLAPENIVITDQ
                     NGVVLNDFSSLADFDRLERTKREQQLIQRLETQYRAAVLRALQQIYTEDRVRDLNIKI
                     DMDMSKKSVQTKEFLPFTLRPDNPTTPYDDSEIVPSVTRSTSETRYEYQGTGFNPEGP
                     AGAEGQTAPAYKDLQNMVGKATQTQTLRNEEINERNTEEDRSPSIDRVTISVNIDGMW
                     TRKIDDKGRPVLRPDGGIEREYEPVPSAELKAAEALIQNAVGYDRARGDSVTVQNIRF
                     DRTAQFRSEDEEFLRRQQVSQIILFSLIGLAVLMVSFIVFRLVSRELERRRRLAEEKR
                     ALEAQMLRENAIRQAEEQSIEVSMSVEERKRLEL"
     gene            complement(1775..2116)
                     /gene="fliE"
                     /locus_tag="P5313_10950"
     CDS             complement(1775..2116)
                     /gene="fliE"
                     /locus_tag="P5313_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRY80923.1"
                     /translation="MAVNNLLPVDIASGDLIRLARTSPLHYTSSGQLGNGAAAKGPSF
                     EDALLKAMDGVNAYQTENTSLVQQMLVDPDSVDAHDVTLAMSRANMSLNIARTVLDRV
                     VRAWRDLINTR"
     gene            complement(2120..2578)
                     /gene="flgC"
                     /locus_tag="P5313_10955"
     CDS             complement(2120..2578)
                     /gene="flgC"
                     /locus_tag="P5313_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRY80924.1"
                     /translation="MGLFSSINIAATGMSAERLRSDVIADNIANASTTRTPEGGPFRR
                     SRVVLRPRVDQPYFRLPFLPKQWDNGVGRGVRVSEVQKDQSSPTRLVYDPTHPDAIQS
                     GPRAGYVEYSNVNIVTEMVDLIAASRAYEANSAIINGSKSMFLKALEIGR"
     gene            complement(2602..3021)
                     /gene="flgB"
                     /locus_tag="P5313_10960"
     CDS             complement(2602..3021)
                     /gene="flgB"
                     /locus_tag="P5313_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRY80925.1"
                     /translation="MFEGNAFGKTVDLLQRSMDVALLRRDVIADNMANSEVPNFKRSD
                     VNFEAALKRALESEQVKPVLDLAVTDERHMPLWRAKDYRTVEPRRVLDYLSTSKNNGN
                     NVDPEQELMASLQNQLMYTLMAQAVNFEFQQVNLVLR"
     gene            complement(3168..4541)
                     /gene="hslU"
                     /locus_tag="P5313_10965"
     CDS             complement(3168..4541)
                     /gene="hslU"
                     /locus_tag="P5313_10965"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRY80926.1"
                     /translation="MTDDFTRLTPARIVEELDRYIVGQLKAKKAVAIALRNRMRRRKL
                     PEEVREEIAPKNILMMGPTGVGKTEIARRLARLCGAPFLKVEATKYTEVGYVGRDVES
                     MIRDLMSAGFQMVKSEMLESVRAEAERRVEDRILDILLPGIKSAAEPVGASQITVANP
                     RSEGADTRERFRSMLREGRLEDREVEVLVQASQGPSLELFAGRQMEGMEEAFSSISGL
                     FGAKKKRRTVTVREARAILLAEETDKLIDPDKVAEEARSRVQEMGIVFIDEIDKVATR
                     EGARAGGIDVSREGVQRDILPIVEGSQVNTKYGIVDTTHILFIAAGAFNVSKPQDLIP
                     ELQGRFPIRVELDALSAQDFRRILTEPENALTLQYRQLLGTEGIELVFEEKAIDRLSE
                     IAARANSQAENIGARRLQTVMERLLEEVSFTAPEKSGTKVVVTPDFVEERFKDVFEKP
                     DLAKYIL"
     gene            complement(4538..5071)
                     /gene="hslV"
                     /locus_tag="P5313_10970"
     CDS             complement(4538..5071)
                     /gene="hslV"
                     /locus_tag="P5313_10970"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRY80927.1"
                     /translation="MKIRSTTVLAVRRGGKVAMAGDGQVTMGATVMKSNAKKVRKVYD
                     GKVLVGFAGATADAFTLFDHFEAKIKEYSGDLTRAAVELAKDWRTDKMLRRLEALLLV
                     ADAEKTLLLSGTGDVIEPAESVIAIGSGGPYAYAAALAYLDAGDLSAAEIAKRSLGIA
                     AGICIYTNAQVTVEELP"
     gene            complement(5068..5964)
                     /locus_tag="P5313_10975"
     CDS             complement(5068..5964)
                     /locus_tag="P5313_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HRY80928.1"
                     /translation="MHRRIEAYLAYLAAVRNLSPRTVESYGNDLASWEAFLALKGRDV
                     EDASTQEVRGFMAARVQAGFAASSVNRALSAVKGLYRYFQRFPGPAGSGTDPARDVEG
                     LPAGRPLPRFLFEDEMGELIDSASGSDFAEVRDRALLELLYSTGCRVSEVAGLRLSDV
                     DLAKGSARVTGKGRKERVVFLADSARAALAAWLPFRAAKAGKEAPWLFVNARGNRLST
                     RGIAWLLDRRAAVLGLGKRISPHAFRHSFATHLVGRGADVRVVQELLGHANISTTQVY
                     THVDLQRLREVYERAHPHGSKR"
     gene            complement(5948..8161)
                     /gene="topA"
                     /locus_tag="P5313_10980"
     CDS             complement(5948..8161)
                     /gene="topA"
                     /locus_tag="P5313_10980"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRY80929.1"
                     /translation="MVDTETTSTAGSSADKSGIKVKTATRKKATSKAKASKKRSGTLV
                     IVESPAKAKTIEKYLGGSYTVLASMGHLIDLPKSRLGIDPDRDFEPEYLTIRGKAKLL
                     KELQKAAKKNRTVLLASDPDREGEAISFHIRNALLAKDPDLAIRRIEFNEITPQAIRE
                     AVGHPRELDVSRVNAQKARRVLDRLVGYHLSPLLWKKVKNGLSAGRVQSVALRLICER
                     EAEVESFIPEEFWTLEASFRKGRHVFSGELVQWKGEKPELKGEEAVKAIEEAVTGAEA
                     TVRDIRETEKTLRPKPPFTTSKLQQTAANRLGFTSKKTMQIAQQLYEGVNIGSTRIGL
                     ITYMRTDSVRLSQTALEDARRWIGQNFPAELPAEPIVYKTDKKAQDAHEAIRPTTVEY
                     TPDKVKDHLTKDQLRLYTLIWERFVSCQMTNAKLRSTALDIGAGEGIFRVNASRVVEK
                     GFYKVLKISTTKEDKETLLPDMAIGEVLKVEALERSQHFTQGPSRFTDASIIRILEEK
                     GIGRPSTYAPIISVLLERYYVTRENRQLAPTTLGRMINEILVDSFPAVVDVGFTAGME
                     EMLDHVEDERRDWVGAIRDFYGPFKSRVDELMESLESLRGRLDEATDIVCEKCGKPMV
                     KKLGRFGFFLACTGFPECRNTRSIPLAKCPRPGCGGEIVARRKAGGRGREFYGCTRYP
                     ECDFLTYYKPTHTNCPKCGQFLVERYDKKRGPYKACINPQCDYLHSQEDEHAPED"
     gene            complement(8167..9150)
                     /gene="dprA"
                     /locus_tag="P5313_10985"
     CDS             complement(8167..9150)
                     /gene="dprA"
                     /locus_tag="P5313_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRY80930.1"
                     /translation="MKGDRDRRRFRLALDRVPFLRARDKIRADSVLDPGAGLSVLSPE
                     RLETLLGRRLGPRSRSRTARGAGPLAWTPVIWETQAEKDLEWFESSGADFRAYGDPEY
                     PPVLRETSRPPFLVYYRGELPDPETPLLGMVGTRYPTGRGLAEAERLAAEAARLGIPV
                     ISGLARGIDAACHRGSLEAGGRTWAVLGCGIDSVYPPRNRDLAARMIRTGGGLLSEYP
                     PGTEPLRYHFPERNRILAGLCRAVLVVEAPRDSGALITADFALEEGRDVLVAEACLES
                     PRSEGCARLYADGARAVSDLMELAEEWGSSPDRRYAARDGSLFAQTTERGW"
     gene            complement(9128..9871)
                     /locus_tag="P5313_10990"
     CDS             complement(9128..9871)
                     /locus_tag="P5313_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80931.1"
                     /translation="MRRTGVSFTLLLAAAALLSSCGGDSDLKKIFDLEERSTKGAPPS
                     TIEDLKKGIAQYSREADRTIEATERIGTYWRLLSVRYMERRMYIEAYEAAKEALRYFP
                     DNTGLYYTIGLGLSYQAKIDSVGGVAAQALRAERLAAAEASFLRALEFNPRYGRALYA
                     LSVLYVFELDRPEDAGPHLTRLLSIETQNVDAMFLLARVRYSEGRLEDAANLYDSILA
                     TTKVEEKRKQAEENKKIVLDLLYEGRPRP"
     gene            complement(9996..10874)
                     /locus_tag="P5313_10995"
     CDS             complement(9996..10874)
                     /locus_tag="P5313_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HRY80932.1"
                     /translation="MDDLLERYRAYLLTARRRSPLTAEVYLREARGLAQRLSERGSDI
                     RTVTPRELLEYLVERRSSGLSSTTMARVVSSVRSLFRFLRLENLRVDDPSELIETPRL
                     ERALPDVLALEEIDRLLASIDVSDPGGVRDRALFELIYSCGLRISEAAGLTFDSLFLK
                     ERMLRVTGKRRKERIVPFGENAARWLALYLDTARPALAKGRRSDRVFLNHLGKGISRK
                     GVWKRFSQVRDLSGVGGKVHTLRHSFATHLLAGGADLRTVQELLGHSDIATTQIYTHV
                     DPEALGDYHREYFPRR"
     gene            complement(10879..>11502)
                     /locus_tag="P5313_11000"
     CDS             complement(10879..>11502)
                     /locus_tag="P5313_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRY80933.1"
                     /translation="KEAFIMADDVLRQAVQGISDLITIPGIINIDFADVKTTMEGQGD
                     AIMGIGSASGDNRAVEAAEKAISNPLLEDARIDGARSILINVSGGEDLSLTEFQEIVN
                     LVSENADPDALIIPGVVHDDCLGDEVRVTVIATGFQRSLKAADSTVRQETRRGAEDYL
                     TPDEWKRITEVPSQKKQYLMGRNDPEDELDIPTVLRDKRYQAERNEA"
BASE COUNT         1728 a         3874 c         3773 g         2127 t
ORIGIN      
        1 ggagttccag gcgcttgcgc tcctccacgg acatggacac ctcgatgctc tgctcctccg
       61 cctgacgtat ggcgttctcc cggagcatct gggcctccag ggcgcgcttt tcctccgcca
      121 gcctcctgcg ccgttcgagc tcccgggaga ccagacggaa cactatgaag gataccatga
      181 gaacggccag tcctatcagg ctaaatagga tgatctggct gacctgctgc cgccggagga
      241 attcctcgtc ctccgagcgg aactgggcgg tgcggtcgaa gcggatgttc tggaccgtga
      301 ccgagtcgcc ccgggcgcga tcgtacccga cggcgttctg gatcagggcc tccgcggcct
      361 tcaactccgc ggagggcacg ggctcgtatt cccgctcgat cccgccgtcg ggcctcagca
      421 ccggccggcc cttgtcgtcg atcttgcggg tccacatccc gtcgatgttc acggagatcg
      481 tgacccggtc aatgctgggc gaccggtcct cctcggtgtt ccgctcgttg atctcctcgt
      541 tccggagggt ctgggtctgg gtcgccttgc ccaccatgtt ctggaggtcc ttgtaggccg
      601 gggcggtctg tccctcggcc cccgcgggtc cctcggggtt gaagcccgtg ccctggtact
      661 cgtaccgcgt ctcgctggtg gaccgggtca ccgaggggac gatctcggaa tcgtcgtagg
      721 gggtggtcgg gttgtccggg cgcagggtga agggcaggaa ttccttggtc tgcacggact
      781 tcttgctcat gtccatgtcg atcttgatgt tcaggtcccg gacccggtcc tcggtgtaga
      841 tctgctggag ggcccggagc accgcggcgc ggtactgggt ctccaagcgc tggatgagct
      901 gctgctcccg cttggtccgc tccagccggt cgaagtccgc gaggctcgaa aagtcgttca
      961 gcacgacccc gttctggtcc gtgatgacga tgttctccgg cgccagtccc cccacggcga
     1021 actggatgat cttctggaca ccctccagct tcttgcggtt ggccgtgatg tcgctgcccg
     1081 gcttgggata gaggatgacg ctggcggtca cgggattctg gtcctcggtg aagagctcct
     1141 tttccgggat ctcgatcacc acgttggcct tgtccacgtc cccgaggctc tcgatgtgcc
     1201 gccggacctc ctggacgacc gccctgcgga ggttgacgtt ccgctcgaag tccgtgaccg
     1261 tgaaccggtc gatggcgaag accgaccagg gatccgtgcc cttggggatc aggtcctccc
     1321 ggagcaggac cgcgttggcc tgggcccgcg tcttctcgtc cttcacgtac aggacgttcc
     1381 cctccccgac ctcgaagggt atgcgctcct cgtccaggcg gctggtgatg agccggagct
     1441 cgtcgtccga ccggatgggc gtggagatga gccgcaccat cgtgggcgcg ctggaaaccc
     1501 ggaagaggag cacggtcccc gcgaccgcgg ccagcacgat gcccgcgagg atgagtttct
     1561 ggaccagggt ccacttggac cacaaggtct tgagctgttc gaagaatttc ttcatccatt
     1621 cgttcatgtt cccctcccaa gttagatgaa cgaaaaacgt gaatagtgaa tagtaaacag
     1681 tgaatcgggg gcatcaactc ggatgcgctt caccaaaccg ggtctcgcgc ctgctttatc
     1741 gtccccctat tcaccccttc acccgttcac ccattcacct cgtatttatc aaatccctcc
     1801 aggcccggac cacccggtcc aggacggttc gggctatgtt caacgacatg ttggcccggc
     1861 tcatggccag ggtcacgtcg tgggcatcca cggaatcggg atcgacgagc atctgctgga
     1921 ccagggaggt gttctccgtc tggtaggcgt tcaccccgtc catggccttg agcagggcgt
     1981 cctcgaagga gggccccttg gcggcggcgc cgttcccgag ctgtcccgag gaggtgtaat
     2041 ggagcgggct cgtccgggcc agccggatga ggtcgccgga cgcgatgtca acgggcagaa
     2101 gattgttcac cgccattcac tacctcccga tctccagggc tttcaggaac atggacttgg
     2161 atccgttaat gatggccgag ttggcctcgt aggcgcggga ggcggcgatg aggtccacca
     2221 tctccgtcac gatgttcacg ttggaatact ccacgtaccc cgcccggggt ccgctctgga
     2281 tggcgtccgg atgggtcgga tcgtagacca ggcgggtcgg cgaggactgg tccttctgca
     2341 cttccgacac ccggaccccc cggcccaccc cgttgtccca ttgcttgggc aggaagggca
     2401 ggcggaagta gggctggtcc acccggggac gcaggaccac ccggctgcgc cggaagggcc
     2461 cgccctcggg ggtgcgggtc gtggaggcgt tggcgatgtt gtccgcgatg acgtcggagc
     2521 ggaggcgctc cgcggacatc cccgtggcgg ctatgttgat cgaactgaac agacccatac
     2581 gctatctcct gtttcctttc atcatctcaa caccaggttg acctgctgga actcgaagtt
     2641 cacggcctgg gccatcaggg tgtacatcag ctggttctgc agggaggcca tgagctcctg
     2701 ctcgggatcc acgttgttac cgttgttctt ggacgtggac aggtagtcca ggactcgccg
     2761 gggctccacg gtccggtaat ccttggcgcg ccagagcggc atgtggcgct cgtccgtgac
     2821 ggccaggtcc aggacgggct tcacctgctc ggattccagg gcccgcttca gggccgcctc
     2881 gaagttgacg tccgagcgct tgaagttggg cacctccgag ttggccatgt tgtccgcgat
     2941 cacgtcccgg cgcaggagcg ccacgtccat ggaccgctgg aggagatcca ccgtctttcc
     3001 gaacgcgtta ccctcgaaca ttccgctctc ctagttcccc gcaaacctcg gtttccgaac
     3061 accgaaaacc ctgtttagta gatcggacga attttcgata tcgtgaatag gtgaattgtg
     3121 aatatgtgaa tagatacttc cattcaccca ttcacctatt tacccattca caagatatac
     3181 ttcgccagat ccggcttctc gaacacgtcc ttgaaccgtt cctccacgaa gtccggggtc
     3241 acgaccacct tcgtgcccga cttctcggga gccgtgaagg aaacctcctc caggaggcgc
     3301 tccatcacgg tctggagcct ccgggccccg atgttctcgg cctgggaatt ggcccgggcc
     3361 gcgatctccg acagccggtc gatggccttc tcctcgaaca cgagctcgat accctcggtg
     3421 cccagtagtt ggcggtactg gagggtcagg gcgttctcgg gttccgtgag gatgcggcgg
     3481 aagtcctggg cggacagggc gtccagctcc acccggatgg ggaagcgacc ctggagctcg
     3541 gggatcaggt cctggggctt ggagacgttg aaggcccccg cggcgatgaa caggatgtgg
     3601 gtggtgtcca cgatcccgta cttggtgttc acctggctgc cttccacgat gggcaggatg
     3661 tccctctgga ccccctcccg ggagacgtcg atccccccgg cccgggcccc ttcccgggtg
     3721 gccaccttgt cgatctcgtc gatgaagacg atccccatct cctgcacccg ggaccgggct
     3781 tcctcggcca ccttgtccgg gtcgatgagc ttgtccgtct cctccgccag gaggatggcc
     3841 cgggcctccc ggacggtcac ggtccggcgc ttcttcttgg ccccgaagag gcccgagatc
     3901 gaggaaaagg cctcctccat cccctccatc tgccggcccg cgaagagctc caggctgggg
     3961 ccctgggagg cctggaccag aacctcgacc tcccggtcct ccaggcggcc ctcccggagc
     4021 atggagcgga agcgctcccg ggtgtcggcc ccctcgctcc ggggattggc cacggtgatc
     4081 tgggaggccc cgacgggctc ggcggcgctc ttgatcccgg gcaggaggat gtccaggatc
     4141 cggtcctcca cccggcgctc cgcctcggcc cggacggact ccagcatctc cgacttcacc
     4201 atctggaacc cagcggacat gaggtcccgg atcatggact ccacgtcccg gcccacgtag
     4261 ccgacctcgg tgtacttggt ggcctccacc ttcaggaagg gggcgccgca gagccgggcc
     4321 aggcgccggg ctatctcggt ctttccgacc cccgtcgggc ccatcatgag gatgttcttg
     4381 ggggcgatct cctcccggac ctcctcgggg agcttgcggc ggcgcatccg gttgcgcagg
     4441 gctatggcca cggccttctt ggccttgagc tgtccgacga tgtaccggtc cagctcctcc
     4501 acgatccggg ccggagtcag gcgggtgaag tcgtccgtca cggcagctcc tccacggtca
     4561 cctgggcgtt ggtgtagatg cagatccccg ccgcgatgcc cagggagcgc ttggcgatct
     4621 ccgccgcgga caggtccccg gcgtccaggt aggccagggc ggcggcgtag gcgtagggac
     4681 ccccggagcc gatggcgatg acgctctccg cgggctcgat gacgtccccc gtcccggaca
     4741 ggagcagggt cttctccgcg tccgccacca ggaggagggc ctccaggcgg cggagcatct
     4801 tgtcggtccg ccagtccttg gccagctcca ccgccgcccg ggtcaggtcc ccggagtact
     4861 ccttgatctt ggcctcgaag tggtcgaaga gggtgaaggc gtccgccgtg gcccccgcga
     4921 aaccgaccag gaccttgccg tcgtagacct tccgcacctt cttcgcgttg ctcttcatga
     4981 ccgtggcgcc catggtcacc tggccgtccc cggccatcgc gaccttgccg cctcgccgca
     5041 ccgccaggac cgtcgtggat cgtatcttca tcgcttgctc ccgtgaggat gcgcccgctc
     5101 gtagacttcc cgcaggcgct gcaggtccac gtgggtgtag acctgggtcg tggatatgtt
     5161 cgcgtgcccc aggagttcct ggaccacccg cacgtccgcc ccccgcccca ccaggtgggt
     5221 cgcgaaggaa tgccggaagg cgtgggggga gatccgcttc cccaggccca ggaccgcggc
     5281 ccgccggtcc aggagccagg cgatcccccg ggtggagagg cggtttcccc gggcgttcac
     5341 gaagagccag ggggcttcct tcccggcctt ggcggcccgg aaaggaagcc aggcggccag
     5401 ggccgcacgg gcggaatcgg ccaggaagac gacccgttct ttgcgccctt tgcccgtcac
     5461 ccgggcggag cccttggcca ggtccacgtc cgagagccgg agaccggcca cctcggagac
     5521 ccggcatccc gtgctgtaca ggagctccag gagggcccgg tcgcgtacct ccgcgaaatc
     5581 gctccccgaa gcggaatcga tgagctcgcc catctcgtcc tcgaagagga accggggcag
     5641 gggacgcccc gcgggaagcc cctccacgtc ccgggcggga tccgtcccgg aaccggcggg
     5701 gcccgggaag cgctggaaat accggtacag gcccttcacg gcggacaggg cgcggttcac
     5761 cgaggaggcc gcgaagcccg cctggacccg ggcggccatg aagccccgca cctcctgggt
     5821 cgaggcgtcc tccacgtcgc gtcccttcag ggccaggaag gcctcccagg aagccaggtc
     5881 gttcccgtag gactccacgg tccggggcga gaggttccgc accgcggcca ggtaggccag
     5941 gtaggcctca atcctccggt gcatgctcgt cctcctggga gtgcaggtag tcgcactggg
     6001 ggttgatgca ggccttgtag ggcccccgct tcttgtcgta tcgctccacc aggaactgtc
     6061 cgcacttggg gcagttcgtg tgggtgggct tgtagtaggt caggaagtcg cactcgggat
     6121 agcgggtgca gccgtagaac tcccggcccc ggcctcccgc cttgcgccgc gccacgatct
     6181 cccctccgca gcccggtcgg gggcacttgg ccagggggat ggaccgggtg ttccggcact
     6241 ccgggaagcc cgtgcaggcc aggaagaacc cgaagcggcc cagcttcttg accatgggct
     6301 tgccgcactt ctcgcagacg atgtccgtgg cctcgtccag gcgtccccgc agggactcga
     6361 ggctctccat gagctcgtcc accctggact tgaaggggcc gtagaagtcc cggatggccc
     6421 ccacccagtc ccgccgctcg tcctccacgt ggtccagcat ctcctccatg cccgcggtga
     6481 agcccacgtc caccacggcc gggaaggaat ccaccaggat ctcgttgatc atccggccca
     6541 gggtggtggg cgccagctgt cgattctcac gggtgacgta gtagcgctcc aggaggaccg
     6601 agatgatcgg ggcgtaggtc gagggcctgc cgatcccctt ctcctccagg atccggatga
     6661 tggaggcgtc ggtgaaccgg gacggcccct gggtgaagtg ctgcgaccgc tcgagggcct
     6721 ccaccttcag gacctcgccg atcgccatgt ccggcaggag ggtttccttg tcctccttgg
     6781 tcgtggagat cttgaggacc ttgtagaagc ccttctccac cacccgggag gcgttcaccc
     6841 ggaagatccc ctctccggct ccgatgtcca gggccgtgga ccggagcttg gcgttcgtca
     6901 tctggcagga cacgaagcgt tcccagatca gggtgtagag ccggagctga tccttggtca
     6961 ggtggtcctt gaccttgtcg ggagtgtatt ccaccgtggt ggggcgtatg gcctcgtggg
     7021 cgtcctgggc cttcttgtcg gtcttgtaca cgatcggttc cgccggaagt tccgcgggga
     7081 agttctggcc gatccagcgg cgagcgtcct ccagggcggt ctgggagagg cgcaccgagt
     7141 ccgtacgcat gtacgtgatg agaccgatgc gggtggagcc gatgttgacg ccctcgtaga
     7201 gctgctgggc tatctgcatg gtcttcttgc tggtaaagcc caggcggttg gccgcggtct
     7261 gctggagctt ggaggtcgtg aaggggggtt tgggccgcag ggtcttctcg gtctcccgga
     7321 tgtcccgcac cgtcgcctcg gcccccgtga cggcctcctc gatcgccttg accgcttcct
     7381 cgcccttcag ttcgggcttc tcgcccttcc actggaccag ctccccggaa aagacgtggc
     7441 ggcccttccg gaaggaggct tccaaggtcc agaactcctc ggggatgaag gattccacct
     7501 ccgcctcgcg ctcgcagatg agcctcaggg ccacggactg gacccgtccc gcggacaggc
     7561 cgttcttgac cttcttccac aggaggggcg agaggtggta ccccacaagg cgatccagga
     7621 cgcggcgggc tttctgggcg ttcacccggg agacgtcgag ctcccgggga tggcccaccg
     7681 cctcccggat ggcctgggga gtgatctcgt tgaactcgat cctccggatg gccaggtccg
     7741 ggtccttggc caggagggcg ttccgtatgt ggaaggagat ggcctcccct tcccggtccg
     7801 ggtccgaggc caggagcacg gtgcggttct tcttggccgc cttctggagc tccttgagga
     7861 gcttggcctt gccccggatg gtcaggtatt cgggctcgaa atcccggtcc gggtcgatgc
     7921 ccagccggga cttgggcagg tcgatcaagt ggcccatgga ggccaggacc gtgtagctgc
     7981 cgcccaggta cttctcgatc gtcttggcct tggcggggga ctccacgatc accagggttc
     8041 cgctccgctt cttcgaggcc ttggccttgg acgtcgcctt cttccgggtc gcggtcttga
     8101 ccttgattcc gctcttgtcc gcggaggagc ccgccgtcga ggtcgtttcc gtatccacca
     8161 tgtcgctcac catcctcgtt ccgtcgtctg ggcgaacaga ctcccatccc tcgcggcgta
     8221 tcgtcgatcg gggctgctgc cccattcctc ggccaattcc atgaggtccg aaaccgcccg
     8281 ggctccgtcc gcgtaaagcc gggcgcagcc ctcggaccgg gggctttcca ggcaggcttc
     8341 cgccaccagc acgtcccgcc cctcctccag ggcgaaatcc gcggtgatca gggcccccga
     8401 atcccggggg gcttccacga ccaggacggc ccggcagagc cccgccagga tccggttgcg
     8461 ttccgggaag tggtaccgca ggggttcggt gcccggggga tactcggaca gaaggccccc
     8521 gccggtccgg atcatccggg ccgccaggtc ccggttccgg ggcgggtaga ctgaatcgat
     8581 cccgcagccc aggacagccc aggtccggcc tcccgcctcc agactgcccc ggtggcaggc
     8641 cgcgtcgatg ccccgggcca gcccggaaat cacggggatc cccaggcggg cggcctcggc
     8701 ggccaggcgt tcggcctccg cgaggccccg gcccgtggga taccgggtgc ccaccatccc
     8761 taggaggggc gtttccggat ccggaagctc tccgcggtag tacaccagga agggcggccg
     8821 ggaggtctcc cggaggaccg gaggatactc cgggtccccg taggcgcgga aatcggcccc
     8881 ggaggattcg aaccactcca ggtccttctc cgcctgagtt tcccaaatta cgggagtcca
     8941 ggccagggga cccgctccgc gggccgtgcg ggaccgggac cgggggccca accgccttcc
     9001 caggagggtc tccagccgtt cgggcgaaag tactgaaagc ccggccccgg ggtcaaggac
     9061 ggaatccgcc cggatcttgt cccgggcccg gaggaagggc actcggtcca gggcgaggcg
     9121 gaaccgccta cggtctcggt cgcccttcat acagcaagtc cagcacgatc ttcttgttct
     9181 cctccgcctg tttccgcttc tcctccacct tggtagtcgc caggatggag tcgtagaggt
     9241 tcgcggcgtc ctccaggcgg ccctcggaat accgcacccg ggcaaggagg aacatggcgt
     9301 ccacgttctg cgtctcgatc gagagcagcc gggtcaggtg ggggccggcg tcctcgggtc
     9361 ggtccagctc gaagacatag agcaccgaca gggcgtagag ggcccggccg taccgggggt
     9421 tgaactccag ggcccggaga aaggaggcct ccgcggcggc caggcgttcc gcccggaggg
     9481 cctgggcggc gacccctccc acggaatcga tcttggcctg gtaggacaac cccaggccga
     9541 tggtgtagta caggcccgtg ttgtcgggga agtaccgcag ggcttccttg gccgcctcgt
     9601 acgcctcgat gtacatccgg cgttccatat accggacgga caggagcctc caataggtcc
     9661 cgatgcgctc cgtggcctcg atcgtccggt ccgcctcccg ggaatactgg gcgatcccct
     9721 tcttgaggtc ctcgatcgtg gacggggggg ctcccttggt gctcctttcc tccaggtcga
     9781 atatcttctt caggtcggag tccccgccgc aggatgacag gagggccgcc gcggccagga
     9841 gaagcgtgaa ggatacgccg gttcttctca tacactctcc ctcccctact acgtccgccc
     9901 gtgcccgccg cgcttgcctt tccgtgcggc cttcgacggc ccgggccgct ccttaggtct
     9961 cagcgcctcg ggaaaacgcc gggcggcttc gccgctcagc gcctcggaaa atactcccgg
    10021 tggtaatccc ccagggcctc gggatccacg tgggtataga tctgggtcgt ggcgatgtcc
    10081 gagtgtccca ggagttcctg caccgtccgg aggtccgcgc ccccggccag gaggtgggtg
    10141 gcgaaggaat ggcgcagggt atgcaccttg ccgcccacgc cggacaggtc gcggacctgg
    10201 gaaaacctct tccagacccc cttgcgggag attcccttgc ccagatggtt cagaaagacc
    10261 cggtcgctcc ggcgcccctt ggccagggcg ggccgggccg tgtccaggta gagggccagc
    10321 caccgggccg cgttctcccc gaagggcacg atccgttcct tccgcctctt gccggtcacc
    10381 cggagcatcc tctccttcag gaacagcgag tcgaaggtca gccccgcggc ctcgctgatc
    10441 cgcaggccgc aggaatagat cagctcgaac agggcccggt cccgcacgcc gccgggatcc
    10501 gacacgtcga tgctggccag gagccggtcg atctcctcca gggccaggac gtccggcagg
    10561 gcccgttcca gtcggggggt ctcgatgagc tccgaggggt cgtcgacccg gaggttctcc
    10621 agccggagga agcggaagag ggagcggacg ctggacacca cccgggccat ggtggtggag
    10681 gacaggcccg agctcctgcg ctccacgagg tactccagga gttcccgggg ggtcacggtc
    10741 cggatgtccg atccccgctc cgacaggcgc tgcgcgaggc cgcgggcctc ccggaggtag
    10801 acctccgccg tgaggggact gcgcctgcgg gcggtcagga ggtaggcccg gtatcgctcg
    10861 aggaggtcgt ccacgcccct acgcctcgtt cctctccgcc tggtagcgct tgtcccgaag
    10921 gaccgtgggg atgtccagct cgtcctccgg atcgttgcgc cccatgaggt actgcttctt
    10981 ctgggagggc acctccgtga tgcgcttcca ttcgtccggg gtcaggtagt cctccgcgcc
    11041 ccgccgggtc tcctggcgga ctgtggagtc cgcggccttc agggatctct ggaaccccgt
    11101 ggcgatcacg gtgacccgga cctcgtcccc caggcaatcg tcgtgcacga cccccgggat
    11161 gatgagggcg tcgggatccg cgttctcgga gaccaggttc acgatctcct ggaattccgt
    11221 cagggagagg tcctcccctc cggagacgtt gatcaggatg ctgcgggccc cgtcgatccg
    11281 ggcgtcctcc agcagggggt tcgagatggc cttctccgcg gcttccacgg cccgattgtc
    11341 cccggaggcg gagccgatcc ccatgatcgc gtccccttgc ccttccatgg tcgtcttaac
    11401 gtcggcaaag tctatattaa tgatgccggg aatggttata aggtcggaaa ttccctggac
    11461 cgcttggcga aggacgtcat cggccatgat gaacgcttcc tt
//
LOCUS       DAOTEI010000110        11438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_3978_length_11438_cov_4.455416, whole genome shotgun sequence.
ACCESSION   DAOTEI010000110 DAOTEI010000000
VERSION     DAOTEI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3978_length_11438_cov_4.455416"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="P5313_11005"
     CDS             <1..319
                     /locus_tag="P5313_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY80934.1"
                     /translation="AFLGDSVIVAHNASFDLGFLKAAAERAGLPAPANPVADTRDLAR
                     EAFPGLPSYALQNLARSFGLSPGNAHRACDDARTCARLLALCVQAAPAAAVGSGTESP
                     PTP"
     gene            316..1344
                     /locus_tag="P5313_11010"
     CDS             316..1344
                     /locus_tag="P5313_11010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY80935.1"
                     /translation="MNPSPAPEAADRRAFALVLVSCLLLLIEGALVLSAASPGSRGTR
                     SAAAAAPAGRDRAAAGPVSAGRLETAALDLDALRRLSLEEAAEAGRAWARAAAADGLI
                     SERELAAVLPSPAFILARTSPDEYADDIRLSAEGAGAFARAYGPIDEAMPGSNAAGAR
                     LEAALGSIHEVRVSLGAAPGPAGLRAPLPRGQRWIASAEDLKKTHRHALDIFFVGEGE
                     RGSVEKGPLIRSMSPGVVVAAADDWVGGEGAAKYRRGGLTPRAGNGAVVYDPGTRRYY
                     TYFHMHDVSVRPGQWVDAGEVLGRGGNTGTNARKRGHGGHLHLEIYEASEDRALDCWE
                     LRDLVLSL"
     gene            1541..2521
                     /locus_tag="P5313_11015"
     CDS             1541..2521
                     /locus_tag="P5313_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80936.1"
                     /translation="MKRFVLVLAALSILAAGVSAQTLDGFQTAFQTFAEDMAGVLAAN
                     STVGNVWSHAYVGGFPRFGAGLAVGANFVPADSAEPLFAAAGETLPAELANLGIPIPA
                     AALSFKIGLPFLNMDVGVKGGLIPDSASGVLSGQGIEATYETLGINVRYAILKDRLMI
                     PAVSIGASWNYLKGSIATGLGAGDTTYTDGATYSVTVSDPDLTLDWQANTFDFTLQAS
                     KNLLIFTPYVGAGFTIGVASTSGGMAATTTVADYNTSGSNADEIAALEAAGISVSDQG
                     FSVSSEVTTPTIRIYGGTSINILILKLDLMASYVPATQALGAQVMARIQL"
     gene            2656..3720
                     /locus_tag="P5313_11020"
     CDS             2656..3720
                     /locus_tag="P5313_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRY80937.1"
                     /translation="MSTTRVSYDLVVLAGDVGGTNTNLALIGKKGGTFSTLIERHYAT
                     KGETSFLAPLGRFLEEARIEVPGFRPDLACVSGAGPVEGDRIRLTNAPWDIDAQAINR
                     EFGLRTFLINDFTAVSYGVLLLDPADESQLRPLAGRNAAFPEPVPGGVKAVIGAGTGL
                     GVGYIVRVGDRTTAFPSEGGHVCLPVYDEETRAFSRWLEDKYGFPPGAEAGVSGQGIG
                     HLFAFLAERDAAAGRALSAADAAVLALPEAERPARVAAASRVSLLCGRSMEIFVKLYA
                     RCAADASASFLPYGGLYLAGGIASKNEAWFLEDFRFMDMFLRSYRSHIRAILARVPVY
                     IVKDYGISLYGAANAAASLM"
     gene            3755..5269
                     /locus_tag="P5313_11025"
     CDS             3755..5269
                     /locus_tag="P5313_11025"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRY80938.1"
                     /translation="MINEALRRDMESKGIDVDLTLSILKDFNEGFYDSVRPVKAAGVP
                     AVDNRTVISLDKGLSFDLPAEEARRRLSDLDLPLPAAVLDEGGRLRFPQAALAELGER
                     LLPRTAYGILNGGSATSYADRKKNQAFGDDVFAALEGPFEELAPLCEGRPKGITPAYI
                     NPDGTPGASFLQLKLRARLLAALAYRRRYGDPGRPVLPLYQMTSAGTDAELARAYEAY
                     EEEEILRPLAEALGAPACSFRTGIQPMIGAYSHSASGRPRTVFDRAYGKPDNALPLPG
                     GHGQCFRVLAPALRDLRDSGTRYAYLGNVDNLGYLPDPVEIGLLALSGQPAAFDFSVR
                     TPVDVKGGILVVTAEGRRLVADIGPAIPFAELQRLEDQGATVLFNCATGLFDLDWLVP
                     RLDEVARALPVRFTDQDKDAGRYSQAEQVTWEVTGLLPSFLAFAVNKYDRFIAAKMLA
                     ETLLTSGVGADDPRLPADLAATSSLLRAGLEWKLAGSYGMELWDGRWTPRDAAY"
     gene            complement(5266..7413)
                     /locus_tag="P5313_11030"
     CDS             complement(5266..7413)
                     /locus_tag="P5313_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY80939.1"
                     /translation="MILPTFCGKDCGGNACPLAVEVEDGRPVRILRSPAAPPELAGCR
                     RGLDLPAFHLSSDRILEPLIRVGDRGSRDFRAASWEEALTLVARRLDEIRTRHGPASI
                     LNLSSSGSTGALHDTQTLTTRFLNAGGGSTVLTGNYSNGAARFVLPYLFGPDAARSGF
                     DPATLRHSRLILLWGANVLEARLGTELPARLLEARRNGARVVCIDPRYTDTARRTDAW
                     WIPVRPGTDAALMLAILHVLLSEGLADADFARSRSLGFDELSAYVLGGDGGPSRDPAW
                     AEGVCGVPAAEIRRLAREYGRTKPALLLPGYSIQRVYAGEDPFRLSVALQLATGNFGR
                     PGGSTGSINNRLPGPRVGSLDELRRPENPGVPVLRWPDAILEGRSGGYPSDIRAAYVC
                     GSNFLNQGGDIGKNVRAFRALDFAVCHEMFLTPTARHCDVVLPAASPLEKEDIGIPWA
                     GNWLAYKRAACPFRGSARTDYDIFSDLAERLGFGDAFTEGRSARDWLDSFLAESEVPD
                     PEAFRETGVYLAPDQERTGLADFAESPRARPLGTPSGLVELSSRAWARDTGLPPIPVW
                     RPAPEEPGRPLLLVTPKSPLRTHSQGGDPGSPAARGGVVLSLHPRDAAARGILPGAEV
                     RIRNGRGSTRAVAELTESILPGTVSLYEGSWFEAESGASESFATAVSDLGGSANVLTG
                     TEGSPPSTACVMHGIPVEVERSGPTGPVGRSGT"
     gene            complement(7424..9772)
                     /gene="lon"
                     /locus_tag="P5313_11035"
     CDS             complement(7424..9772)
                     /gene="lon"
                     /locus_tag="P5313_11035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRY80940.1"
                     /translation="MPDNEIIPIDQLLPAKIPIIPLMGKPIFPGIFTPIMVGNGDDVG
                     TVDEAMQADGIVGLVMIKNEFDKPTSQDLHQVGTVAKIVKRINLPDGSVNIFISTLKR
                     FKVKKYLTKQSPIVAAVEYLDEEIEDADEVKALTRALLSEMKQVSENNPLFSEEMRLN
                     MVNIDHPGKIADFIASILNLDKNEQQKILEMLDVKDRMERVLLFIKKEQELLKIQKRV
                     QQEINERIEKNQREYFLKEELKAIKGELGMPTDSKSSDYQRFKDKIEGFAFQGEVKEL
                     VEQELEKFNLMDPNSSEFIVTRNWLDLISNLPWKAAVNGDFDLKKAQDILESDHYGLK
                     DVKDRIIEYLAVRKLKKDAKGSILCLVGPPGVGKTSVGRSIARALGKEFFRFSVGGMR
                     DEAEIKGHRRTYVGALPGKIVQGLKITKSRDPVFMIDEIDKMGVSYQGDPSSALLEAL
                     DPEQNFAFRDHYLDLPFDISHVFFIVTANTLDSIPGPLIDRMEVIQLPGYVDTEKLEI
                     ARHYLIPKSMEKNGMKKGTVRYTRDALMFIAESYAREAGVRNFEKYLDKIHRKIAKSI
                     VLEDKGGTKFEVDKPAVQKYLGKPLFREDEVKRATRPGMSVGLAWTSMGGDTLIIEAV
                     ANPGKEGFKLTGQMGAVMQESAGIAYTHVRHLAAESYGVGADYFEGRQIHLHIPEGAT
                     PKDGPSAGITMATALLSLVLGRKIKDRLAMTGELSLTGQVLPIGGLKEKTVAARRNKI
                     REIIIPKANEKDLDEIPEHVKKGIAFHPVETMEEVIAIALPS"
     gene            complement(9897..10856)
                     /locus_tag="P5313_11040"
     CDS             complement(9897..10856)
                     /locus_tag="P5313_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRY80941.1"
                     /translation="MELVGIGNALLDVISFVEPDYPPYLGVHNSTTNHVPYDRMEDLL
                     LAVPDPIFVAGGGAANTIRVAALLGLQGRFVGAVGRDRFGDLFRRDMEEAGAEFLAVE
                     KSQPTGIFLALVGEDGSRAIVVSPGAAGELSVSEIPDRTFVPGAILYVDGFIASRREL
                     FIHILERGRRAGMRVAVDAASFSLANRNAAFFLDAFEEYCDWVFMNEDELRALAGLPL
                     DEALSLFAASRFEMIVKRAERGAIWCYGDRRIDSPVRAFTPMDETGAGDAFAAGFLAA
                     VLEGKPPEVCLRMGNRVAEEIIQVPGCRLDPSRIRKARQSVLV"
     gene            11066..11139
                     /locus_tag="P5313_11045"
     tRNA            11066..11139
                     /locus_tag="P5313_11045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11100..11102,aa:Val,seq:tac)
BASE COUNT         1790 a         3890 c         3806 g         1952 t
ORIGIN      
        1 ggccttcctg ggggactcgg tgatcgtcgc ccacaacgcc tccttcgacc tgggcttcct
       61 caaggccgcg gcggagcggg cgggtctgcc cgccccggca aaccccgtgg cggacacccg
      121 ggacttggcc cgggaggcct tcccgggcct gccttcctat gccctccaga acctggcccg
      181 ctccttcggc ctgtcccccg gaaacgccca ccgggcctgc gacgacgccc gcacctgcgc
      241 ccgcctcctg gccctctgcg tacaggcggc cccggccgcg gccgtaggct ccggaacgga
      301 gagccccccg acgccatgaa cccgagtccc gccccggaag ccgcggaccg ccgcgccttc
      361 gccctcgtcc tcgtatcctg cctcctcctg ctgatcgagg gggccctggt cctttccgcc
      421 gcgtccccgg gttcccgcgg aacgcggtcc gcggcggccg ccgctccggc cggacgggac
      481 agagcggccg cgggccccgt ctccgccggc cgcctcgaga ccgccgccct agacctcgac
      541 gccctccgcc ggctctccct ggaggaggcc gccgaggcgg gaagggcctg ggcccgggcg
      601 gccgcggcgg acgggctcat ctccgagcgg gaattggccg ctgtcctccc ctccccggcc
      661 ttcatcctgg cccgcacctc cccggacgag tacgcggacg acatccggct gtccgccgag
      721 ggcgcaggcg cctttgcccg ggcgtacggg cccatcgacg aggccatgcc cggatcgaac
      781 gccgcgggag cccggctgga ggccgccctg ggctcgatcc acgaggtccg ggtctccctg
      841 ggcgcggctc cgggtcccgc cgggctccgg gctcccctgc cccggggaca gaggtggatc
      901 gcctccgccg aggacctcaa gaagacccac cgccacgccc tggacatctt cttcgtcggg
      961 gaaggggaac gcgggagcgt cgaaaagggc ccgttgatcc ggtccatgag ccccggggtc
     1021 gtggtggccg ccgcggacga ctgggtcggg ggcgagggcg ccgccaagta ccggcggggc
     1081 ggcctcaccc cccgggccgg aaacggggcg gtggtctacg accccgggac ccggcggtac
     1141 tacacgtatt tccatatgca cgacgtctcc gtccggcccg gccagtgggt ggatgccggc
     1201 gaggtcctgg gccggggcgg gaacaccgga accaacgccc gcaagcgggg ccacggcggt
     1261 cacctccacc tggagatcta cgaggcctcc gaggaccggg ccctggactg ctgggaactc
     1321 cgggacctgg tgttgtcgct ttgacccctt ccagaacgtg gaaccagagt aaggacagga
     1381 ttaacaagat gataaacaga tgaacaagat agagaataac tattttatat atatacattt
     1441 atcctgtata tccgaattcc atcctgtcca tcctgtcaaa tatttctgaa aaacccgacc
     1501 ggcgcgctat acttagaggg cgacccaagg aggtttcacg atgaaacggt ttgttctagt
     1561 ccttgcggcc ctgtcgatcc tcgccgcggg cgtgtcggcc cagactctcg acggattcca
     1621 gaccgccttc cagaccttcg cggaggacat ggccggggtc ctggccgcca actccacggt
     1681 cgggaacgtc tggtcccacg cctacgtggg gggcttcccc cgcttcggcg ccggactggc
     1741 cgtgggcgcc aacttcgtgc ccgcggattc cgcggagcct ctcttcgctg cggcgggcga
     1801 gactctgccc gctgagctcg cgaatctcgg cattcccatc cccgccgcgg ccctgtcctt
     1861 caagatcggg cttccattcc tgaacatgga cgtgggtgtc aagggcggcc tcatcccgga
     1921 ctcggcttcc ggagtattgt cggggcaggg aatcgaggct acttacgaaa cattgggaat
     1981 caatgtaagg tatgcgattc tcaaggatcg cttgatgatt ccggccgtat ccatcggggc
     2041 gagctggaat tatcttaagg gatctatagc cacgggatta ggcgcaggag atactacata
     2101 cacagacggt gcaacatatt cagttactgt ctcggatccc gatctaacac ttgactggca
     2161 ggcgaacaca ttcgatttca cccttcaagc ctcaaagaac cttttaatct tcacgccgta
     2221 tgtcggcgca ggttttacca ttggtgttgc cagtacgagc ggaggtatgg cagcaacaac
     2281 gactgtagca gattataata cgagtggtag caatgcggat gaaatcgctg ctttggaagc
     2341 tgctggaatt tccgtttctg atcaagggtt ttccgtctct tcagaggtga ccaccccgac
     2401 gatccgtatt tacggcggca ccagcatcaa catcctgatc ctcaagctgg acctcatggc
     2461 ctcctacgtc ccggccaccc aggccctggg cgcccaggtg atggcccgga tccagctcta
     2521 gatcccgact cgaacgattc ggtgcgcggc cgccctccag ggcggccgct tccaattttc
     2581 acgcccccgc acccgacgag accgcctcgg tacgccccgg tttgacagga tcggttcccc
     2641 gtatatgatg tacccatgag caccacgcgc gtgagctacg acctggtcgt cctggccggg
     2701 gacgtgggcg gcaccaacac caacctggcc ctgatcggca agaagggcgg aaccttctcc
     2761 accctgatcg aacggcatta tgccacgaag ggcgagacct ccttcctggc ccccctgggc
     2821 cgcttcctgg aggaggcccg gatcgaggtc cccggcttcc ggcccgacct ggcctgcgtc
     2881 tccggggccg ggcccgtgga gggcgaccgg atccgcctga ccaacgcccc ctgggacatc
     2941 gacgcccagg cgattaaccg ggaattcggg ctccggacct tcctgatcaa cgacttcacc
     3001 gccgtctcct acggcgtcct cctcctggat cctgcggacg agtcccagct ccgccccctg
     3061 gccggccgga atgcggcgtt ccccgaaccg gtccccggcg gggtcaaggc cgtcatcggg
     3121 gcgggcacgg ggctgggggt gggctatatc gtacgcgtgg gggaccggac gacggccttc
     3181 ccctcggagg gggggcacgt atgtctcccg gtctacgacg aggagacccg ggccttctcc
     3241 cggtggctcg aggacaagta cggcttcccg cccggggcgg aggcgggggt ctccgggcag
     3301 ggtatcggcc acctcttcgc cttcctggcg gagcgggacg cggccgccgg ccgggccctc
     3361 tccgcggcgg acgcggccgt gctggccctt ccggaggcgg agcgtccggc ccgggtcgcg
     3421 gcggcctccc gggtgagtct gctctgcggg cgatccatgg agatcttcgt gaagctctac
     3481 gcccgctgcg ccgcggacgc ctcggcctcc ttccttccct acgggggcct ctacctggca
     3541 gggggcatcg cctcgaagaa cgaggcgtgg ttcctggagg acttccggtt catggacatg
     3601 ttcctccgga gctaccgttc ccacatccgg gcgatcctgg cccgggtacc cgtgtatatc
     3661 gtgaaggact acggcatctc cctgtacgga gcggcgaacg ccgcggcttc cctgatgtga
     3721 acacggccgg atccgcggcc atggaggaac caggatgatc aacgaggcac tgcgccggga
     3781 catggaatcc aagggcatcg acgtggacct gaccctttcg atcctcaagg acttcaacga
     3841 gggcttctac gactccgttc ggccggtcaa ggccgcgggg gtccccgcgg tggacaaccg
     3901 cacggtcatc tccctggaca aggggctgtc cttcgatctc cccgcggagg aggcccggcg
     3961 ccgcctttcg gacctggacc tgcccctgcc cgccgccgtc ctggacgagg gcggacggct
     4021 ccgcttcccc caggccgccc tggccgaact gggggagcgg ctcctcccca ggacggccta
     4081 cgggatcctg aacggcggat ccgccaccag ctacgcggac cgaaagaaga accaggcctt
     4141 cggggacgac gtcttcgccg ccctggaggg ccccttcgag gagctggctc ccctctgcga
     4201 gggccgaccc aaggggatca ccccggccta catcaacccc gacggaaccc ccggtgcgtc
     4261 cttcctccag ctgaagctcc gggcccggct cctggcggcc ctggcctatc gccgccgcta
     4321 cggagacccg gggcggcccg tcctgcccct ctaccagatg accagcgccg ggaccgacgc
     4381 cgagctggcc cgggcctacg aggcctacga ggaggaggag atcctgcgcc ccctggccga
     4441 ggccctcggc gccccggcct gctccttccg gaccgggatc cagcccatga tcggggccta
     4501 ttcccactcc gcatcggggc gccccaggac ggtcttcgac cgggcctacg ggaagccgga
     4561 caacgccctc cccctgcccg gcggccacgg ccagtgcttc cgggtcctag cgccggccct
     4621 ccgggacctg agggactcgg ggacgcgcta cgcctacctg gggaacgtgg acaacctcgg
     4681 ctatctgccg gacccggtgg agatcggcct tctggccctg tccgggcagc ccgcggcctt
     4741 cgatttctcg gtgcggaccc ccgtggacgt gaagggaggg atcctggtgg tgaccgcgga
     4801 gggccggcgt ctggtcgcgg acatcggtcc ggccatcccc ttcgcggagc tccagcgcct
     4861 ggaggaccag ggtgccacgg tcctgttcaa ctgcgccacg gggctcttcg acctggactg
     4921 gctggtaccc cgcctggacg aggtggcccg ggcccttccg gtccggttca cggaccagga
     4981 caaggacgcg ggccgctact cccaggccga gcaggtcacc tgggaagtca ccgggctcct
     5041 gccgtccttc ctggccttcg cggtgaacaa gtacgaccgc ttcatagcgg ccaagatgtt
     5101 ggcggagacc ctgctgacca gcggcgtggg ggcggacgac ccgaggctgc ccgccgacct
     5161 ggcggctacc tccagcctcc tccgggcggg actggaatgg aaactggcgg gctcctacgg
     5221 gatggagctc tgggacggac gctggacgcc ccgggacgcc gcctactagg ttccggaccg
     5281 gccgaccgga cctgtcggac ccgatcgttc cacctccacc ggtatcccgt gcatcacgca
     5341 ggccgtggag ggagggctgc cctccgtccc ggtcaggacg ttcgcggacc cgcccaggtc
     5401 cgagaccgcc gtggcgaagg attccgacgc gccggattcc gcctcgaacc aggagccctc
     5461 gtacaggctg acggtacccg gaaggatgga ttccgtgagc tctgcgacgg cccgggtcga
     5521 cccccggccg tttcggatcc tcacttccgc gcccggcagg atgccccggg ccgcggcgtc
     5581 ccgggggtgc aggctcagga cgacccctcc ccgggcggcc ggggagccgg ggtccccgcc
     5641 ctgggagtgg gtgcggagcg gcgacttggg ggtcacgagg aggaggggac ggccgggctc
     5701 ctcgggcgcc ggcctccaga cggggatcgg cggcagtccc gtgtcccggg cccaagcccg
     5761 gcttgagagc tcgacaaggc ccgagggggt gcccagggga cgggcccggg ggctctccgc
     5821 gaagtccgcc agccccgtcc gctcctggtc cggggcaaga tagactcccg tttcccggaa
     5881 ggcctccggg tcgggtacct ccgactccgc caggaaggag tccagccagt cccgggcgga
     5941 ccttccctcc gtgaacgcat ccccgaagcc caggcgctcc gccaggtccg agaatatgtc
     6001 gtagtccgtc cgggccgagc cccggaatgg acaggcggcg cgcttgtagg ccaaccagtt
     6061 gccggcccag gggataccga tgtcctcctt ctccagggga gaggccgcgg gcaggaccac
     6121 gtcgcagtgc cgggccgtgg gggtcaggaa catctcgtgg cagaccgcga agtccagggc
     6181 ccggaaggcc cggacgttct ttccgatgtc gccgccctgg ttcaggaagt tcgagccgca
     6241 gacgtaggcg gcccggatgt ccgaaggata gcccccggag cgtccctcca ggatcgcgtc
     6301 gggccagcgc aggacgggca ccccggggtt ctccggcctg cggagctcgt ccaggctgcc
     6361 gacccggggt cccggcaggc ggttgttgat ggagcccgtg gaaccgcccg gtctcccgaa
     6421 gttgcccgtg gccagctgga gggcgacgga gaggcggaag ggatcctctc cggcatagac
     6481 ccgctggatc gagtaccccg ggaggaggag ggccggcttg gtccgcccgt actcccgggc
     6541 cagtcgccgg atctccgccg cggggacccc gcagacgccc tcggcccagg ccggatcccg
     6601 agagggtccg ccgtcccctc ccaggacata ggcggacagc tcgtcgaaac ccagggaccg
     6661 ggagcgtgcg aagtccgcgt ccgccagtcc ctccgacaga aggacgtgga gtatcgcaag
     6721 catcagggcg gcgtccgtgc ccggacggac gggaatccac caggcgtccg tccggcgggc
     6781 ggtgtccgtg taccgggggt cgatgcagac gaccctcgcg ccgttccgcc gggcctccag
     6841 gagccgggcg ggaagctcgg tccccagccg ggcctccagg acgttggcgc cccataggag
     6901 gatcagccgg gaatgccgca gggtcgccgg atcgaatccg gatcgggcgg cgtccggtcc
     6961 gaagaggtag ggcaaaacga accgcgccgc cccgttggag tagttgcccg tgaggaccgt
     7021 cgagcccccc ccggcgttca ggaacctcgt cgtcagggtc tgggtgtcgt ggagggcccc
     7081 ggtggatccc gagctggaga ggttcaggat cgaggcggga ccgtgccggg tccgtatctc
     7141 gtccaggcgc cgggcgacca gggtcagggc ctcctcccag gaggcggcgc ggaagtcccg
     7201 ggaaccccga tccccgacgc ggatcaaggg ctccagtatg cggtcggaag agaggtggaa
     7261 ggccggcagg tccaggcccc ggcggcaccc cgccagctcg gggggggccg cggggctgcg
     7321 gaggatccgc accggtcggc cgtcctctac ctccaccgcc agggggcagg cgttgccccc
     7381 gcagtccttc ccgcagaagg tgggcaggat cataccggtc ccgtcacgac ggcagggcta
     7441 tggctatgac ttcctccatg gtctccacgg ggtggaaggc gatacccttc ttcacgtgct
     7501 cggggatctc gtccaggtcc ttctcgttgg ccttggggat gatgatctcc cggatcttgt
     7561 tcctccgggc ggccacggtc ttctccttga ggcccccgat gggcaggacc tggccggtca
     7621 gggaaagctc cccggtcatc gccaggcggt ccttgatctt ccggcccagg accagggaca
     7681 ggagggccgt ggccatggtg atccccgcgg agggtccgtc cttcggggtg gccccctcgg
     7741 ggatgtggag gtggatctgc cggccctcga aatagtccgc ccccacccca taggactccg
     7801 cggccaggtg ccgcacgtgg gtgtatgcga tgccggcgga ttcctgcatg accgccccca
     7861 tctggccggt cagcttgaag ccttcctttc cgggattggc caccgcctcg atgatcaggg
     7921 tatccccgcc catgctggtc cacgccaggc ccacggacat cccgggccgg gtggcccgct
     7981 tgacctcgtc ctcccggaac aggggcttgc ccaggtactt ctggaccgcg ggcttgtcca
     8041 cctcgaattt cgtccccccc ttgtcctcca ggacgatgga cttggcgatc ttgcggtgga
     8101 tcttgtccag gtacttctcg aagttgcgga ccccggcctc ccgggcgtag gactcggcga
     8161 tgaacatcag ggcgtcccgg gtgtagcgga cggtgccctt cttcatgccg ttcttctcca
     8221 tggacttggg gatcaggtag tgccgggcga tctccagctt ctcggtgtcc acatagcccg
     8281 gcagctggat gacctccatc cggtcgatca ggggacccgg gatggaatcc agggtgttgg
     8341 ccgtgacgat gaagaagacg tgggagatgt cgaagggaag gtccaggtag tggtcccgga
     8401 aggcgaaatt ctgctcgggg tccagggcct ccaggagggc gctggaagga tccccctggt
     8461 agctgacccc catcttgtcg atctcgtcga tcatgaagac cggatcccgg ctcttggtga
     8521 tcttgagtcc ctggacgatc ttcccgggca gggcccccac gtaggtccgg cggtgcccct
     8581 tgatctccgc ctcgtcccgc atcccgccca cggagaatcg gaagaactcc ttgcccaggg
     8641 cccgggcgat ggaccgtccc acgctggtct tcccgacccc cggaggtccc accaggcaga
     8701 ggatggagcc cttggcgtcc ttcttgagct tccggaccgc caggtattcg atgatgcggt
     8761 ccttcacgtc cttgaggccg tagtggtcgg attccaggat gtcctgggcc ttcttgaggt
     8821 cgaaatcccc gttgaccgcc gccttccagg gcaggttgct gatcaggtcc agccagttcc
     8881 gggtgacgat gaactccgag gagttggggt ccatgaggtt gaacttctcg agctcctgct
     8941 ccaccagctc cttgacctcc ccctggaagg cgaagccctc gatcttgtcc ttgaacctct
     9001 ggtagtccga gctcttggag tccgtgggca tgcccagctc gcccttgatg gccttgagct
     9061 cctccttgag gaagtattcc cgctggttct tctcgatccg ttcgttgatc tcctgctgga
     9121 cccgcttctg gatcttgagg agctcctgtt ccttcttgat gaacaggagg acccgctcca
     9181 tccggtcctt gacgtcgagc atctccagga tcttctgctg ctcgttcttg tccaggttca
     9241 ggatgctggc gatgaaatcc gcgatcttgc ccggatggtc gatgttgacc atgttgagcc
     9301 gcatctcctc ggagaagagg ggattgttct cggaaacctg tttcatctcc gacaggaggg
     9361 cccgggtcag ggccttgacc tcgtcggcgt cctcgatctc ctcgtccagg tattcgaccg
     9421 cggccacgat gggactctgt ttcgtcaggt acttcttgac cttgaagcgc ttcagcgtgg
     9481 agatgaagat gttgaccgag ccgtccggga ggttgatccg cttgacgatc ttggctaccg
     9541 tcccgacctg gtgaaggtcc tgggaggtgg gcttgtcgaa ctcgttcttg atcatcacga
     9601 gtcctacgat cccgtcggcc tgcatggcct cgtccacggt gccgacgtcg tccccgttcc
     9661 ccaccatgat gggggtgaag attccgggga atatgggttt tcccatgagg ggaatgatcg
     9721 ggatcttggc gggcagaagt tgatcgatgg ggatgatctc gttgtccggc attgcaggct
     9781 cctttcacag ggttacgacc tataaaatac gaggtttgac cgatacgggc aagattccgc
     9841 aagcgtttta gggaagtttg acgggatttc agggttgttg gacaggttcg gcggacttat
     9901 accaggacgg actggcgggc tttccggatc cgggaagggt ccaaccggca gccgggcacc
     9961 tggatgatct cctcggccac ccgattgccc atccgcaagc agacctcggg gggcttgcct
    10021 tccaggaccg cggccaggaa gcccgccgcg aaggcgtccc ccgccccggt ctcgtccatc
    10081 ggggtgaagg cccggaccgg gctgtcgatc ctgcggtctc cgtagcacca gatggcgccc
    10141 cgctcggcgc gcttcacgat catctcgaac cgggaggccg cgaagaggga cagggcctcg
    10201 tccaggggca gccccgccag ggcgcggagc tcgtcctcgt tcatgaagac ccaatcgcag
    10261 tactcctcga aggcgtccag gaaaaaggcg gcgttccggt tggccaggga gaagctggca
    10321 gcgtccacgg ctacccgcat ccccgcgcgt cggccacgtt ccaggatgtg gatgaagagc
    10381 tcccttcggg aggcgatgaa gccgtccacg tacaggatcg ccccgggcac gaaggtacgg
    10441 tcggggatct cggagacgga cagctccccc gctgcgccgg gggagacgac gatggctcgg
    10501 gaaccgtcct cccccaccag ggccaggaag atgcccgtgg gctggctctt ctccacggcc
    10561 aggaattcgg cccccgcctc ctccatgtcc cgccggaata ggtccccgaa acggtccctg
    10621 cccacggcgc ctacgaaacg gccctggagt cccaggaggg ccgccacgcg gatcgtgttg
    10681 gccgcgccgc cccctgccac gaagatgggg tccggaacgg ccaggaggag gtcctccatc
    10741 cggtcgtagg ggacgtggtt ggtggtggag ttgtgaacgc ccaggtaggg cgggtagtcg
    10801 ggttccacga aggagatcac atccagcaag gcgttgccga ttccgacgag ttccatggat
    10861 tctcccggct cgaaggacac gatgggatct agaactttac cgaggccttt aaggcagttt
    10921 atcacggttg gcgggaggcg gcaacggttg tcggcccggc gcggtgacgt gcggatcagg
    10981 ccgcctcctt cccggacgga tccggatcgt ccgcaagacc ctccgattga cgaagccggc
    11041 cccccgtgct aatgtccggc gacgcgggcg cttggctcag ctggtcagag cgcctggttt
    11101 acacccagga tgtcgggggt tcaaatccct cagcgcccat aaaaacccga caggccaaag
    11161 gccggtcggg tttttatact taggcgctga gggacaacgt gtgcgcgcac agcgcacgtt
    11221 acaatcgatt ccactcggtg ccagcgcgaa gcgctgattt gaaccagagc gctgtggcga
    11281 gcgagagcga gcccttggcg caggatgcgc caagaagcgc ggaggcgggc tgcagggagc
    11341 gcgcaggatt gcgcgcgacc gtaagccaaa tccctcagcg cccatgccgg ggctcatcgg
    11401 cacgtatcaa tggacggccg tttcgtgtat catggacg
//
LOCUS       DAOTEI010000111        11234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4078_length_11234_cov_5.090259, whole genome shotgun sequence.
ACCESSION   DAOTEI010000111 DAOTEI010000000
VERSION     DAOTEI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4078_length_11234_cov_5.090259"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="P5313_11050"
     CDS             complement(<1..1294)
                     /locus_tag="P5313_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80942.1"
                     /translation="MKVVIVHYHLKPGGVTTVIRRHLDACARAGIEAGLLTGEEPPEP
                     PGVPWEAVPGLAYDPPGEPVPTDKARDRGAALALDLAAAAERLAGSGPFLYHVHNPTI
                     RKNAALCPALSVLSGDGARVLIQAHDFAEDWRPDVLLNRLPYPAGCRWAVLNGRDYRA
                     LRSAGLPESDLELLPNPLPAPGAPTPGAAAGRGDLVLYPVRGIPRKNLGEVILLSRWL
                     PPGLSAAVTLPPSNPKDFPLYEAWKYLARETGARVSFEAGLSASLDALYARTRTAVTT
                     SVKEGFGFSFLEPLARGVPVAGRRLPSVVPDFEATGIPYPALYPAIRVPEGLFSREAF
                     GARLDSALQAVFRALSDALGGEPTWLAGRLAEVRAAALPEGGADFGVLDPAAQAEILE
                     RLYHDPAASAEMERANPFLTSWWDAPPPPPPEALEEYSP"
     gene            complement(1291..1845)
                     /locus_tag="P5313_11055"
     CDS             complement(1291..1845)
                     /locus_tag="P5313_11055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY80943.1"
                     /translation="MPNGSDPWESRGERPIQDFGIFKASEVERRGPHGKTGRFVILDA
                     PDWGVVVPILTRNGARYVLAVRQFRHGSGTVSLEFPGGVVESGEDPERAAARELREET
                     GYTAGRLTLLGDLSPNPAILTNRFRVYLAEDLRPEGDQSLDEHEIVDVREIPEEEFLE
                     SLGGPGWDHALMLAAAFLYVRRRG"
     gene            1957..2301
                     /locus_tag="P5313_11060"
     CDS             1957..2301
                     /locus_tag="P5313_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80944.1"
                     /translation="MDMPDWVTGFPGAVTVSDGNHRILWMNDKAAQVFSSDGGRSLIG
                     GDLMACHNDRSKEILRRMLADGKPNIYTIEKKGVKKLIYQTPWKDESGRVAGLVELSL
                     EIPFEMPHFVRS"
     gene            2413..3114
                     /locus_tag="P5313_11065"
     CDS             2413..3114
                     /locus_tag="P5313_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY80945.1"
                     /translation="MGIQERKNREKEERKTLILAKAKELILEHGIDALSMQEIADGTE
                     LSKATLYLYFQSKEAILKEILAESASAFVEYVESRISPESSGIEALRTLWASYMSLYG
                     ESHEVFVLTGINNFLEPGFLLDLGRTGADLDTPPGRMLAMIARVLERGIADGTLKTDL
                     KPLKLARTVILIATAIIDTVARLPRPARDSRIILAEMRSVFELLIRGLAADGTDRSLL
                     ALPDLDAGRSRNRRP"
     gene            3134..5881
                     /locus_tag="P5313_11070"
     CDS             3134..5881
                     /locus_tag="P5313_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRY80946.1"
                     /translation="MERFFRRPWIIVAVIAAVTVFFAAQLPRARLDNNNFRFVPKEDP
                     ERLAAVRIDEDFGSQMMILVGLERNYGTVLEPDFLESLRAYVKALEAVREIDSVQSVI
                     SSDYVTGTPDSIVVEPLVPEDFSGTRAEIAELRDRLSSWDLYRRSLVSDDFRATQVMV
                     YLDLLSEEAGGEAAQRVFGEVRRLAREAGFPGTEIYLTGIPVFSSVINEAMTADLVFL
                     IPLVVIVVLAVLFLSFRRAGGIVLPLLTVLISTVWAVGAMPLAGIKLSILSTVLPVIL
                     VAVGSAYGIHVISHYYDEMAGAGGVDGETHRRIVFRALRTVRAPVLLAALTTFAGFAS
                     FCFTPVVPIFEFGVFASLGVLAAFGVSVTLIPALLILRGPPRRLPRYRMTEIESGSED
                     PLSAAIADSLCAVARKPRSILALAALAAFLSLFGVTRLVIDNVLVEYFRADTQIVRSD
                     RFIREYFGGSKTISVVVRSPIPGEVLRPDVLSALDGLSVYLQENVPEVGKAAGFTDLV
                     KRMNQILNADESPEGLRASKAAVPEGPPGGAGGGEPAFGFGTEGAPEAGSVPAFGFAE
                     FPEPAETSLPPASAGAGGPGYREDTAVPVSKPLDARGVAELVRVALSEGPSRGLSADQ
                     LADRIYRAVNYRGASYYEIPSDPARYGKTGPEELKALVSNYLVLMAGDLSAFADDPLE
                     PTAIRTGIQLRTLGQIDTDRAVGAIRGYAAERFPKDVTVEIGGAALVEKSLNRLVVQS
                     QLTSVVLSLVLVFLILSIYYRSALAGLVGLAPLSVSVLANFAIMQAAGIKLNIGTAMM
                     ASINVGIGIDYIVHYMAAYHREYLAGGGGEGFLRRTFLTSGKAILFNAASVGAGFAVL
                     VLSRFNMLAELGFLISLAMASSALVSLTVLPVLLSLADPAFIKRPLPADRARSPSEVS
                     P"
     gene            5878..6648
                     /locus_tag="P5313_11075"
     CDS             5878..6648
                     /locus_tag="P5313_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRY80947.1"
                     /translation="MKRIASAAAALALAVLAASAQDAQAIARAARDRIRADTVSTRAR
                     MVITDKSGAQTERLVDQYSATVDGADATMIVFQKPASVAGTRFLTIQAPGKPEDRWIF
                     LPSLGKVRRISAGEGSGSFMGTDFSYDDVSLMDRDVDLDTFTLAREETLDGKACWVLE
                     ARPKDPGYQYSRMLLWIGKADSISWKIEMYDRRDAPVKVLTIDKVQDIQGRLSPVQTT
                     VRSVQAGTSTVLFVDILKYDDKIPPTVFTVRFLETGRP"
     gene            6648..7976
                     /locus_tag="P5313_11080"
     CDS             6648..7976
                     /locus_tag="P5313_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80948.1"
                     /translation="MNPLKRSYPALLLAIALALPSSRAAAQAFGFGTEPSPEDPAAAP
                     ARAALPVEARGTLSLGALLFPEDLSDPAGGKPEDASLLRLDLIAEGNAVQAELRLRIG
                     RSALEEATAAGFLDEAVDEAYLRLFLGTVTLEGGIRKVAWGKADSQGPLDVLNPFDYT
                     DMTVTDDLERKIARPLLRAVWAAAPKVQVEAVFLPAFEPHAVDFTGRWQPAAVRTLLA
                     SGYTGFSAPDTDGLDYAQAGARLAATSGSLDWGLQYFYGYLPTPSYDVSTFPTVTVLY
                     NRYQQFGADLAAVFGGFGVRAELAANLTEDLPGDDPLVYNPRIAFSLGADRGIGAGIN
                     VNLQYAGTVRLLDGGVTAPTDVDSGTDAFASTLTAVASQKLLRDKLEWRLAALWSPSD
                     EDFLLIPSLSWTEGDAEVKLAAGIFGGDPAGQMGQYADASWVRLTLTYAF"
     gene            7981..8289
                     /locus_tag="P5313_11085"
     CDS             7981..8289
                     /locus_tag="P5313_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80949.1"
                     /translation="MRSVVEELMDKIAAESQVGPRPSLGRARSQTRGVRSVARGARSV
                     ARADLGMVLFAEREAIDILWKAADAAVRAEAPAGLEGPEAAALAAAVERLRPLFGPRG
                     "
     gene            complement(8286..8459)
                     /locus_tag="P5313_11090"
     CDS             complement(8286..8459)
                     /locus_tag="P5313_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80950.1"
                     /translation="MGSPEGRGRLEAGDQGKYTKGDGGLSNFPGHGSKVARLFGKAVK
                     EVFEYGEGEGIAS"
     gene            8458..9675
                     /locus_tag="P5313_11095"
     CDS             8458..9675
                     /locus_tag="P5313_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRY80951.1"
                     /translation="MKAFQIADSVYALHADIDSADLFEGIWPIPHGVSLNSYVVKGER
                     TALIDLVRDWCGAPKVLAAELASVGIDLAGIDYLILNHLEPDHTGWLAEFRKLNPKAE
                     ILATAKGIELVKSFYKETEGLRALKSGDTLDLGGGRILAFEEAPNVHWPETMVTWDAK
                     SGTLFSCDAFGSYGKLGDRIFDDQFTEEEHAFFEKETLRYYANIVASFGVFVKRAVQK
                     LAGLDIKVIAPSHGMVWRKDPKRIVERYLTYAGYLDGGREKEICVVWGSMYGNTKKGL
                     DEVLRGIEAEGVPYTLHRVPNEDPSFILADAYKSEGIVIAMPTYEYRMFPPMAYVLSL
                     LERKHVKGRTALRIGSWGWVGGAKKEYDELIVPLKWNCLESVEWAGGAGPETMKTLYE
                     SGRELARRVKSAP"
     gene            complement(9891..10037)
                     /locus_tag="P5313_11100"
     CDS             complement(9891..10037)
                     /locus_tag="P5313_11100"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRY80952.1"
                     /translation="MEAWLDTLRSADTLGKAAGLAVFGMAGVFVTLFLFYLLISALEK
                     LSKK"
     gene            complement(10046..11173)
                     /locus_tag="P5313_11105"
     CDS             complement(10046..11173)
                     /locus_tag="P5313_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRY80953.1"
                     /translation="MEELFAGFTSVGYRQFIMYAIGGVLIWLAVKKEYEPMLLLPIGF
                     GAILTNLPLSFAVGTAQEPGFLRILLDAGIMTELFPILIFIAVGAMIDFGPLFKNPLM
                     IFFGAAAQLGIFATVLLAVALGFDLKVAAAIGIIGAADGPTSIYVAKRFAVQYLGPIS
                     VAAYSYMSLVPIIQPPVIRLLTTKKERTMRMEYREEPVPRLLKILFPILVTMISGFVA
                     PISVPLVGSLMFGNLIRESVVLERLSQTAQNELAYLVTLLLGITIGSTMSADAFLRRE
                     TLLILGMGLLAFVFDTAGGVLFAKFLNLFLPRDRKVNPMIGACGISAFPMSARVIQRM
                     AQEEERGNIILMQAVGANVAGQLGSIVAGGMVLALVPLLVR"
BASE COUNT         1861 a         3821 c         3771 g         1781 t
ORIGIN      
        1 ggggggagta ttcctccagg gcttccggag ggggcggagg cggggcgtcc caccaggatg
       61 tcaggaaggg atttgcccgt tccatctccg ccgaggccgc cggatcgtgg taaagccgtt
      121 ccaggatctc ggcctgggcg gccggatcca ggacgccgaa gtcggctccg ccttcgggaa
      181 gggccgcggc gcggacctcc gccaggcggc ccgccagcca ggtaggctcg cctcccaggg
      241 cgtccgagag ggcccggaaa actgcctgga gggccgagtc cagccgggct ccgaaggctt
      301 cccgggagaa gagtccctcg gggacccgaa tggcgggata cagggccgga taggggatcc
      361 cggtcgcctc gaagtcgggc accacggaag gaagccgcct ccccgcaacg ggcactcccc
      421 gggccagggg ttccaggaag gagaagccga agccttcctt gacggaggtc gtgaccgccg
      481 tccgggtccg ggcatagagg gcgtccaacg acgcggagag tcccgcctcg aaggacaccc
      541 gggctcccgt ttcccgggct aggtacttcc acgcctcgta gagcgggaag tccttgggat
      601 tgctgggggg cagggtcacc gccgcggaca accccggggg cagccatctc gaaaggagga
      661 tgacctcccc gaggttcttc cggggtatgc cccgtaccgg gtagaggacc agatcccccc
      721 ggccggcggc ggcgccgggg gtcggggctc ccggcgcggg aagggggttc ggcaggagtt
      781 ccagatcgga ctccgggagc cccgcggacc gcagggcccg gtagtcccgg ccgttcagga
      841 ccgcccagcg gcagcccgcc ggatagggaa gcctgttcag gagcacgtcg gggcgccagt
      901 cctcggcgaa gtcgtgggct tggatgagga ctcgcgcccc gtccccggac agtacggaca
      961 gtgccgggca gagggcggcg ttcttgcgga tcgtgggatt gtggacgtgg tagaggaagg
     1021 ggcccgagcc tgccagccgc tccgcggcgg ccgccaggtc cagggccagg gcggcccccc
     1081 ggtcccgggc cttgtccgtc gggacgggct cccccggcgg atcgtaggcc aggccgggca
     1141 ccgcctccca gggcaccccc ggcggctccg ggggctcctc ccccgtcagg aggcccgcct
     1201 cgataccggc ccgggcgcag gcgtccaggt gacggcggat caccgtggtc accccgccgg
     1261 gcttgaggtg gtagtggacg atgacgacct tcatccgcgc cgccggacgt agaggaaggc
     1321 cgcggccagc atgagggcat ggtcccagcc cggaccgccc aggctttcca ggaattcctc
     1381 ttccggtatc tcccggacat ccacgatctc gtgctcatcc agggactggt ccccctcagg
     1441 gcggagatcc tcggccaggt agacccggaa gcggttggtc aggatggcgg gattcgggga
     1501 caggtccccc agcagggtca gtcgcccggc cgtgtagccc gtctcctccc ggagttcccg
     1561 ggcggcggcc cgttccgggt cctccccgga ctccacgaca ccgccgggga actccaggct
     1621 gacagtgccg ctcccgtggc ggaactggcg gaccgccagg acgtatcgag ccccgttccg
     1681 cgtcaggatc ggcaccacga ctccccagtc cggggcgtcc aggatcacga agcgccccgt
     1741 cttgccgtgg ggccctctcc gttccacctc ggaggccttg aagattccga aatcctgtat
     1801 tggacgctcg ccccgggact cccagggatc cgatccgttg ggcatggttc cttcatagta
     1861 ttctcttgcc cgggggctgg caagcgcggt tctgtattgt atcctgtcgc cgacccgcgt
     1921 actgcgggcc gtggcagcat cgtcaggagg tacagcatgg acatgcccga ctgggtcacc
     1981 gggtttcccg gagcggtaac ggtttccgac gggaaccaca ggatactttg gatgaacgac
     2041 aaggccgccc aggtcttctc ctcggacggt ggacgatccc tgatcggcgg agacctgatg
     2101 gcctgccaca acgaccggtc caaggagata ctccggcgca tgctggccga cgggaagccg
     2161 aacatctaca cgatcgagaa gaagggggtg aagaagctca tctaccagac cccctggaag
     2221 gacgagtccg gacgcgtcgc cgggctcgtg gagctctccc tggagatccc cttcgagatg
     2281 ccccacttcg tccggagctg aatcccgcgg gctccgatcc ccggtatcca tcctagtccg
     2341 ccttcgaatg acccttgttc tttttctgac caccggttat tatatgatca gcggtaatag
     2401 ccggaggtgc ggatggggat ccaggagcgg aagaaccggg aaaaggaaga gcggaaaacc
     2461 ctgatcctgg ccaaggccaa ggagctgatc ctggagcacg ggatcgacgc cctgtccatg
     2521 caggagatcg cggacggaac ggagttgtcc aaggcgaccc tctacctcta cttccagagc
     2581 aaggaggcga tcctcaagga gatcctcgcg gaatccgcct ccgccttcgt ggagtacgtg
     2641 gagtcccgga tctccccgga gtcctcgggg atcgaggccc tgcggaccct ctgggccagc
     2701 tacatgagcc tgtacgggga atcccacgaa gtcttcgtgc tcaccgggat caacaacttc
     2761 ctggagccgg gcttcctgct ggacctgggc cggaccggag cggacctcga taccccgccg
     2821 gggcggatgc tggccatgat cgcccgggtg ctggaacggg ggatcgccga cgggaccctc
     2881 aagacggacc tcaagcccct caagctggcc cggacggtca tcctgatcgc cacagccatc
     2941 atcgacaccg tggcccgcct gccccgcccc gcccgggact cccgcatcat cctggcggag
     3001 atgaggagcg tcttcgaact gctgatccgg ggcctggccg cggacgggac ggaccggtcc
     3061 ctcctggccc tgcccgacct ggatgccggc cggtcccgga accgccgtcc ctagagaccc
     3121 gagagaggac gttatggaga gattcttccg cagaccctgg atcatcgtcg cggtcatcgc
     3181 ggcggtcacc gtgttcttcg cggcccaatt gccccgggcc cggctggaca acaacaactt
     3241 ccggttcgtc cccaaggaag accccgaacg cctggcggcc gtccgcatcg acgaggactt
     3301 cggcagccag atgatgatcc tggtggggct ggaaaggaac tacgggacgg tcctggagcc
     3361 cgacttcctg gagagcctcc gggcctacgt gaaggccctc gaggccgttc gggagatcga
     3421 ctcggtccag tccgtgatct cctcagacta cgtgaccggc accccggact ccatcgtggt
     3481 ggagcccctg gtcccggagg acttctcggg cacccgggcc gagatcgcgg agctccggga
     3541 ccgcctgagt tcctgggacc tctaccggag gtccctggtg tccgacgact tccgggcgac
     3601 ccaggtcatg gtctacctgg acctcctgtc cgaggaggcc ggaggcgagg ccgcccagag
     3661 ggtcttcggg gaggtccggc gcctggcccg ggaggccgga ttcccgggca ccgagatcta
     3721 cctgacgggc atacccgtct tcagctccgt gatcaacgag gccatgaccg cggacctggt
     3781 cttcctgatc cccctggtgg tcatcgtggt cctggcggta ctcttcctgt ccttccgacg
     3841 ggcgggcggg atcgtcctgc ccctcctgac cgtcctgatt tccacggtct gggccgtggg
     3901 ggccatgccc ctggcgggga tcaagctctc catcctgtcc acggtcctgc ccgtcatctt
     3961 ggtggccgtg gggagcgcct acgggatcca cgtgatcagc cattactacg acgagatggc
     4021 cggagcgggc ggggtcgacg gggaaaccca ccggcgcatc gtcttccggg ccctgcggac
     4081 cgtccgggcc ccggtcctcc tggcggccct gaccactttc gcgggtttcg cctccttctg
     4141 cttcaccccc gtggtcccca tcttcgagtt cggggtgttc gccagcctgg gggtccttgc
     4201 ggccttcggg gtgtccgtca ccctcatccc ggccctcctc atcctccggg gcccgccccg
     4261 gcgccttccc cggtaccgga tgacggagat cgagagcggg tccgaggatc ccctgagcgc
     4321 ggccatcgcg gattccctgt gcgcggtcgc ccgcaaaccc aggtccatcc tggccttggc
     4381 ggccctggcc gccttcctct ccctgttcgg agtcacccgc ctggtcatcg acaacgtcct
     4441 ggtggagtac ttccgggcgg atacccaaat cgtacggtcg gaccgcttca tccgggaata
     4501 tttcgggggt tcgaaaacca tcagcgtggt ggtccggtcc ccgatcccgg gggaggtgct
     4561 ccggcccgac gtcctttccg ccctggacgg gctttccgtc tacctccagg aaaacgtccc
     4621 cgaggtcggg aaggccgcgg ggttcacgga cctggtcaag cggatgaacc agatcctgaa
     4681 cgccgacgaa agccccgagg gactccgggc ttcgaaggca gccgtccccg agggacctcc
     4741 cggcggagcc ggcgggggag aaccggcgtt cggattcgga acggaggggg ccccggaagc
     4801 cggaagcgtc cctgccttcg gattcgcgga gttcccggag cccgcggaaa catccctacc
     4861 cccggcgtcg gcgggcgcgg gaggaccggg ataccgggag gatacggccg tcccggtctc
     4921 gaaacccctg gacgcccggg gcgtggcgga gctggtccga gtcgccctgt ccgaggggcc
     4981 ctcccggggg ctcagcgcgg accagctggc ggaccgtatc taccgggcgg tcaactaccg
     5041 gggagcctcc tactacgaga tcccctcgga tcccgcccgg tacggcaaga cgggcccgga
     5101 ggaactgaag gccctggtct cgaactacct ggtcctgatg gccggtgacc tctcggcctt
     5161 cgcggacgat cccctggagc cgacggcgat ccgcacgggg atccagctcc ggacactggg
     5221 ccaaatcgac accgaccgcg ccgtcggggc catccggggc tacgcggcgg agcggtttcc
     5281 caaggacgtg accgtggaaa tcggcggcgc cgccctggtg gaaaaatccc tgaaccgcct
     5341 ggtcgtgcag tcccagctga cctccgtggt cctctccctc gtgctggtct tcctgatcct
     5401 gtcgatctac taccgttccg ccctcgcggg gctggtgggc ctggcgcccc tgtccgtgtc
     5461 ggtcctggcc aactttgcca tcatgcaggc cgcggggatc aagctgaaca tcgggaccgc
     5521 catgatggcc agcatcaacg tgggaatcgg gatcgactac atcgtccact acatggccgc
     5581 ctaccatcgg gagtacctgg ccgggggcgg gggggaaggc ttcctgcgga ggaccttcct
     5641 gacctccggc aaggccatcc tcttcaacgc cgcctccgtg ggggcaggct tcgcggtcct
     5701 cgtgctctcc cggttcaaca tgctagccga actgggcttc ctgatctccc tggccatggc
     5761 ctccagcgcc ctggtctccc tgaccgtcct tcccgtgctc ctgtccctgg ccgacccggc
     5821 cttcatcaag cggccgcttc ccgcggatcg agcccgttcg ccctcggagg tatccccatg
     5881 aaaagaatcg catccgccgc cgcggcactg gccctggcgg tcctggccgc gtccgcccag
     5941 gacgctcagg cgatcgcccg ggcggcccgg gaccgcatcc gggcggacac cgtgtccacc
     6001 cgggcccgga tggtcatcac ggacaagtcc ggtgcccaga ccgaacgcct ggtggaccaa
     6061 tactccgcca cggtggacgg ggcggacgcc acgatgatcg tgttccagaa accggccagc
     6121 gtggccggca cccgattcct taccatccag gcccccggga agcccgagga ccggtggatc
     6181 ttcctgccct ccctgggcaa ggtgcgccgc atctccgccg gggagggatc cggttccttc
     6241 atgggtaccg acttctccta cgacgacgtt tccctgatgg accgggatgt ggacctggac
     6301 accttcaccc tggcccggga ggagaccctg gacgggaagg cctgctgggt cctggaggcc
     6361 cggcccaagg acccgggtta ccagtactca cggatgctcc tgtggatcgg caaggcggat
     6421 tccatctcct ggaagatcga gatgtacgac cgacgggatg ccccggtgaa ggtcctgacc
     6481 atcgacaagg tccaggacat ccaggggcgc ctctcgcccg tgcagaccac ggtgaggagc
     6541 gtccaggccg ggacgtccac ggtcctcttc gtggacatcc tcaagtacga cgacaagatc
     6601 cccccgacgg tcttcaccgt gcggttcctg gaaaccggga ggccctgatg aaccccttga
     6661 aacgctccta ccccgccctt ctcctcgcga tcgccctggc ccttccctcg tcccgggccg
     6721 ccgcccaggc cttcggattc gggaccgagc cctccccgga ggatcccgcg gccgccccgg
     6781 cccgggcggc cctccccgtc gaggctcgcg gcacgctgtc cctgggtgca ttgctcttcc
     6841 cggaggacct ttcggatccg gccgggggga aacccgagga cgcgtccctt ttgcggctcg
     6901 acctgatcgc cgagggaaac gcggtccagg cggagctccg gctccggatc ggcagatccg
     6961 ccctggagga agccacggcg gcgggcttcc tggacgaggc ggtggacgag gcctatctcc
     7021 ggctcttcct gggaacggtg accctggagg gcgggatccg gaaggtcgcc tggggcaagg
     7081 cggactccca gggccccctg gacgtcctca atcccttcga ctatacggac atgaccgtga
     7141 cggacgacct cgagcggaag atcgcccgcc ccctcctgcg cgccgtctgg gccgcggccc
     7201 ctaaagtaca ggtggaggcg gtgttcctgc cggccttcga gccccacgcc gtcgacttca
     7261 cgggccggtg gcagcccgcc gcggtccgga cgctcctggc ctccggatac acgggcttct
     7321 cggcgccgga cacggacggc ctggactacg cccaggcggg agcgcgcctt gccgcgacct
     7381 ccggctccct ggactgggga ctccagtatt tctacgggta cctgccgact ccctcctacg
     7441 acgtgtccac cttcccgacg gtgacggtcc tctacaaccg ctaccaacag ttcggggcgg
     7501 acctggccgc ggtcttcgga ggcttcggcg ttcgggccga gctggccgcg aacctcacgg
     7561 aggacctccc gggggacgac cccctggtct ataacccgcg gatcgccttc tctctggggg
     7621 cggaccgggg aatcggggcg gggatcaacg tgaacctcca gtacgccgga accgtccggc
     7681 tcctggacgg cggggtcacc gcgccgacgg acgtggactc ggggacggac gccttcgcgt
     7741 ccaccctcac cgccgtggca tcccaaaagc tcctccggga caagctcgag tggcgcctgg
     7801 ccgcgctctg gagcccgtcg gacgaggatt tcctgctcat tcccagcctg tcctggaccg
     7861 agggggacgc ggaggtgaaa ctggccgcgg ggatcttcgg cggagatccg gccgggcaga
     7921 tgggccagta cgcggacgcc tcctgggtgc ggctgaccct gacctacgcg ttctgaaacc
     7981 atgcgaagcg tcgtcgagga actgatggac aagatcgccg cggagtccca ggttgggccc
     8041 cggccgagcc tgggccgagc ccggagccag acccggggag tccggagcgt ggcccgggga
     8101 gctcggagcg tagcccgagc ggacctgggt atggtcctct tcgcggagcg ggaggcgatc
     8161 gacatccttt ggaaagccgc ggacgcggcg gtccgggcgg aggcgccggc gggactcgaa
     8221 ggcccggagg cggcggcgct tgccgcggcg gtggagcgcc tccggcccct cttcggcccc
     8281 cggggctagg aggcgatccc ctcgccctcc ccgtactcga agacctcctt gacggccttt
     8341 ccgaagagtc gggccacctt ggagccgtgt ccaggaaaat tcgacagccc tccgtcccct
     8401 ttggtatact taccttggtc cccggcctcg agccggccgc gaccctcagg agaacccatg
     8461 aaagccttcc agatcgcgga ttccgtatac gccctccacg cggacatcga ctcggccgac
     8521 ctcttcgagg gcatctggcc cataccccac ggggtgtccc tgaattccta tgtcgtcaag
     8581 ggggagagga cggccctgat cgacctggtc cgggactggt gcggggcccc caaggtcctg
     8641 gcggcggaac tcgcctcggt cgggatcgac ctggccggga tcgactacct catcctgaac
     8701 cacctggagc cggaccacac gggctggctg gcggaattcc gcaaactcaa ccccaaggcg
     8761 gagatcctgg ccaccgccaa gggcatagaa ctggtgaaga gcttctacaa ggagaccgag
     8821 ggcctccggg cgctcaagtc cggggacacc ctggacctgg ggggcggcag gatcctggcc
     8881 ttcgaggagg cccccaacgt ccactggccg gagaccatgg tcacctggga cgccaagagc
     8941 gggacccttt tctcctgcga cgccttcggc agctacggga agctcgggga ccggatcttc
     9001 gacgaccagt tcaccgagga ggagcatgcc ttcttcgaga aggagaccct ccgctactac
     9061 gccaacatcg tggccagctt cggggtcttt gtgaagcggg cggtccagaa gctcgcgggc
     9121 ctggacatca aggtcatcgc ccccagccac gggatggtct ggcggaagga cccgaaacgg
     9181 atcgtggagc ggtacctcac gtacgcgggc tacctggacg gcgggcggga gaaggagatc
     9241 tgcgtggtct ggggctccat gtacgggaac acgaagaagg ggctggacga ggtcctgcgg
     9301 gggatcgagg ccgagggagt gccctacacc ctccaccggg tgcccaacga ggatccctcc
     9361 ttcatcctgg cggacgccta caagagcgag gggatcgtca tcgcgatgcc cacctacgag
     9421 taccggatgt tcccccccat ggcctacgtg ctctcgctcc tggagcggaa gcatgtcaag
     9481 ggcaggacgg ccctgcggat cgggagctgg ggctgggtcg ggggcgccaa gaaggagtac
     9541 gacgagctga tcgtccccct gaaatggaac tgcctggaat ccgtggagtg ggcgggggga
     9601 gcgggcccgg agacgatgaa aaccctctac gagagcggcc gggaactggc ccgtcgggtc
     9661 aagtcggcac cctgaaaaaa tgggccggcc gacttcgcgt cgaccggccg tgcccgacgg
     9721 ggccgcttga actgactgaa tgagccccag acttttttac atgaccacaa ggaggtttcg
     9781 gagaggcgta ccgccgctcc gggaggacgg acgcttaccc gaccgcgccg ccctcctctc
     9841 cattacaaac cgctatgcgg attctgtcaa cagaaacatc ccggaagcgc tcactttttc
     9901 gacagttttt ccagggccga gatgagaaga tagaacagga atagagttac gaagaccccg
     9961 gccatgccga agacggccag tccggcagcc tttcccagtg tatccgcgga cctcagggta
    10021 tccagccaag cttccatata tcctcctacc ggaccaggag gggcactagg gccagcacca
    10081 tgccccccgc cacgatggac cccagctgac ccgccacgtt ggcgcccacg gcctgcatga
    10141 ggatgatgtt cccccgttcc tcctcctggg ccatcctctg gatgacccgg gcggacatgg
    10201 gaaaggccga gattccgcag gccccgatca tgggattcac cttccggtca cggggcagga
    10261 agaggttcag gaacttggcg aagagcaccc ctccggcggt gtcgaagacg aaggccagga
    10321 gccccatgcc caggatcagc agggtttccc ggcgcaggaa ggcatcggcg ctcatggtgg
    10381 acccgatggt gatgcccaaa aggagggtga ccaggtaggc cagctcgttc tgggcggttt
    10441 gggagagccg ctccaggacc acggactccc ggatgaggtt gccgaacatc agggagccca
    10501 ccaggggcac gctgatgggg gccacgaagc cggagatcat ggtcaccagg atggggaaga
    10561 ggattttaag cagccggggc accggctcct cgcggtactc catgcgcatc gtccgctcct
    10621 tcttcgtggt caacaggcgg atgaccggag gctggatgat gggcaccagg gacatgtagg
    10681 agtaggccgc cacggagatg gggcccaggt actggacggc gaagcgcttg gccacgtaga
    10741 tgctcgtggg gccgtccgcg gccccgatga tcccgatggc cgccgcgacc ttgaggtcga
    10801 agcccagggc aacggccagc aggaccgtcg cgaagatgcc cagctgggcg gccgccccga
    10861 agaagatcat cagggggttc ttgaacaggg ggccgaaatc gatcatggcc cccacggcga
    10921 tgaagatgag gatcgggaag agctcggtca tgatccccgc gtccaggagg atccggagga
    10981 agcccggctc ctgggcggtc cccaccgcga aggacagggg caggttggtc agaatggccc
    11041 cgaacccgat gggcaggagg agcatgggct cgtactcctt cttgaccgcc agccagatca
    11101 ggacgccccc gatggcgtac atgatgaact ggcggtagcc gaccgacgtg aacccggcga
    11161 acagctcttc catctacctg ttcctttcat ccagaacggc cagcccctcc agggacagcc
    11221 aaggatcgaa gaac
//
LOCUS       DAOTEI010000112        11147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4139_length_11147_cov_5.083213, whole genome shotgun sequence.
ACCESSION   DAOTEI010000112 DAOTEI010000000
VERSION     DAOTEI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4139_length_11147_cov_5.083213"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="P5313_11110"
     CDS             <1..449
                     /locus_tag="P5313_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY80954.1"
                     /translation="LGYLERALRPAATAWDIMTEEVEVLGQEDSLLDASLFFERTMHT
                     GAPVVDAEGNLSGIITLRDILKGRKAGQMKAPVRTYMSRNVVTAAPDSTVRQIDDLLF
                     RHNIGHLPVVVDRKVVGIVTRTDFLEYKHNERRKKDQLLKEFREEA"
     gene            complement(472..1182)
                     /locus_tag="P5313_11115"
     CDS             complement(472..1182)
                     /locus_tag="P5313_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80955.1"
                     /translation="MLKLESVSTFYGNIQALKGISLEIKQGEIVTLIGANGAGKSTTL
                     MSVCGVVPPREGKVFFEDRDITGLSSDRIVELGIVQVPEGRRIFPGLTVKENLDMGAY
                     LRKDKPGIAKDFEYVFELFPRLAERRNQAGGTLSGGEQQMLAISRALMGRPKLLLLDE
                     PSLGLAPILVQQIFKIIEQVNRDQKTTIFLVEQNANLALKTAHKGYVMETGRITLSDT
                     AANLLANAEVKRAYLGGH"
     gene            complement(1175..1966)
                     /locus_tag="P5313_11120"
     CDS             complement(1175..1966)
                     /locus_tag="P5313_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY80956.1"
                     /translation="MSESILDAKNVTMNFGGLRALNDVSMTIRQGEIVALIGPNGAGK
                     TTFFNCVTGVYPPSAGTISITPPGKSPEVINNLLPHVVTEKGLARTFQNIRLFQNMTV
                     LENVMIGRHARLKAGILGAILRDGSTREEEQKVIHDSYEILKKIELEKYVNELAKNLP
                     YGAQRLLEIARALATEPFLLLLDEPAAGMNPKETGHLDDLIVRIRDTEKVSIFLIEHD
                     MSLVMRVSERIYVVDYGQLIAEGTPAEIKRNPVVIKAYLGEEVNA"
     gene            complement(1959..3206)
                     /gene="livM"
                     /locus_tag="P5313_11125"
     CDS             complement(1959..3206)
                     /gene="livM"
                     /locus_tag="P5313_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HRY80957.1"
                     /translation="MNKLIPELKKSFVTALWFAFLTFPILVIKVNTVRNSILWRWGNL
                     AAVFVLTMALSYVWRRLLEKRARGERLFDLSGLVPAKLRGIELKSVFAQKKNSRAALA
                     VLLALAVLFPFVAGMYQTNIVTTALMYVILGLGLNIVIGLGGLLQLGYAAFYAVGAYT
                     YGILNTRFGLSFWIALPLGAVLATIFGIILGFPVLRLKGDYLAIVTLGFAEICRIVLE
                     NWNDFSSGPSGISQIDRPPFFGIKLSVPQSAIYLYLITLALVVVTVFIVRRLENSRLG
                     RAWEAMREDAVASEAMGVNLTIAKLSAFALGAFWAGMVGVLFAAKTTFINPASFTLME
                     SVLILCIIVLGGMGSTAGVIVGALVIILLPEYLRAFTQYRMLLYGSILVLMMVFRPGG
                     IIRKVRKSYVFKADGQVEGAGHE"
     gene            complement(3218..4126)
                     /locus_tag="P5313_11130"
     CDS             complement(3218..4126)
                     /locus_tag="P5313_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611057.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HRY80958.1"
                     /translation="MDLNYLLQLFISGMTRGSIYALIALGYTMVYGIVQLINFAHGEI
                     YMIGAFTALIVATVLNMTGMPGIAVFAIAMVVAVVYSAAYGFTMEKIAYKPLRNAPRL
                     SALISAIGMSYFLQNYVLLAQTSDFVSFPRLIPKFRFLEGLGGMLSSTQVIIFLVTAV
                     VMVLLTLLIKFTKIGKAMRATAQDKTMALLVGININQVIAVTFVIGSAMAAVGGVLIS
                     SHVGQINFYIGFIAGIKAFVAAVLGGIGSIPGAVLGSFVLGLTESFGTGYISSDYEDV
                     FAFIILVLILIFRPSGLLGKSQKEKI"
     gene            complement(4331..5452)
                     /locus_tag="P5313_11135"
     CDS             complement(4331..5452)
                     /locus_tag="P5313_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY80959.1"
                     /translation="MKKRLVILTALAAIALLAFVGCADKGPKTIKIGVAGAHTGDLAS
                     YGLPSVKAAELVVEEWNAKGGVLGKKIELVVADDVCKPEVATNTASKLVGDKVIAVIG
                     HICSGATKAALGIYKDAKIITISPSATNPPLTKSGDYPNFFRTIASDDAQAEVDVEFA
                     MKKLGFKKFAVLHDKGDYGKGFAEFAKGYLDAAGAEVLVFEGIQVGAVDYSAIINKVA
                     ASGAEAILYGGYHPEASKLVSQMRAKGMKTVFISDDGVKDDTFIKVAKEAAEGVYASG
                     PVDTTKNPLAVKATEDHKKKYGEDPGAFFLNAYAATQALLNAIQKAGSVDYDAVTKAL
                     RSEFVETPLGKISFDAKGDAIGVGFSVYQVQNGVYVEIK"
     gene            complement(5623..6465)
                     /gene="rlmJ"
                     /locus_tag="P5313_11140"
     CDS             complement(5623..6465)
                     /gene="rlmJ"
                     /locus_tag="P5313_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRY80960.1"
                     /translation="MLGYRHAFHAGNHADILKHLVLYHCLRYMTKKPKPLLYLDTHAG
                     AGAYRLEEGYAAQTREWESGIKRLSGEATAPVPPEAVAEYLEFIQEYGGIHPGEYPGS
                     PALAAALLRPGDRLSLREIHPADLEELRIRFAGDGRVDVQGTDGFASVRAVLPPPSRR
                     GLVILDPPYELASDYDRVVEALAEALGRFETGTYMVWYPLLERPEARALPARLSALTT
                     RQKLSAELRVRDIGPGDRGLAGSGIFVVNPPWPLESALRSALPFLADTLGQDPAASGR
                     LETT"
     gene            6562..8151
                     /locus_tag="P5313_11145"
     CDS             6562..8151
                     /locus_tag="P5313_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HRY80961.1"
                     /translation="MAAVGFHHRRSSGIRGTLSRWYGSRVGIFLQSILIGVLGGFTVV
                     LFRLLLEEADRFRAAAYLFLRGAGPGYIAAWIAVLIAGGLLLGWACGRWPMIRGSGIP
                     QIKGALLRKMRLEWLPELPLKILTGVLGIGLGLSLGREGPSVQIGAYVGKGVLGVARR
                     PVVERKYLITSGAAAGLAAAFNAPLAGVLFALEELHKHFSPLLLACAMGASVTGDFVA
                     SRFFGLGPAFDFHQIVPLPLTKLPWVLVFGVLCAFLGDLFKRSLYAAQDLYPAIRIPP
                     AARPALALLVSVPLGFFLFDVTGGGHALIERLWGESFSLGFLLLLLAAKIAFSALSYG
                     SGTAGGIFLPLLACGALAGKLFGTVLADLGWALPGDSANFMILGMAALFTGVVKAPVT
                     GAVLILEMSGNFNHFAGLMACCLTAFVTADLIKSKPVYDVLLERLLARNPGAFKPVAG
                     RKIVLEIPVAEGSRLSHRLVRNLPWPEDCLVVGVERGEADLIPHGSTEILPGDRLHVL
                     SDEEDAGEVKEILLRMGEREE"
     gene            8315..9268
                     /locus_tag="P5313_11150"
     CDS             8315..9268
                     /locus_tag="P5313_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80962.1"
                     /translation="MKKFLVLLLVLAALAPAFADDAKVLPKGVFRLSFVFARNAYDER
                     YDANGDISSTGEVSALAIGTALEFGVTDQVTAAVQWTPAYAFNTSIESVAPLPFDPTL
                     ANADGLADLFVGAKFLIMGAQGFVPNDTFRIAFAPGVLIPLDNPDWAAEVAAAIGGNE
                     YKVTSPSTQAIGLGFRTYFDWVLSKMFYINLYNQTIFYLPVTKSQFNPLDTTEHEYAY
                     GYKLTFEAEPHFDYSFSDKVSVSVGVPVTFTMGPQLEIDGAPSATNPEYYILSASPSA
                     SVFVMAFMPFELKAGYTLPILGKNANAASTLVFQLKTFLKF"
     gene            complement(9334..10782)
                     /locus_tag="P5313_11155"
     CDS             complement(9334..10782)
                     /locus_tag="P5313_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRY80963.1"
                     /translation="MMKKVFIAFLAAASAAAIAAQAPAESSSPRSIVFLGFQSEDVTP
                     SGLAILSELVRREISIAPGFSLVERADLDALLREQELGLSDAFDPKGASRIGRLAGAQ
                     SLMVGRIGILGTLYIISLRIVDVETGTVERAMTEEFMGNLEDIRKPVRIAAQKILRIP
                     GIEVQQGEYIRVETEPAGVAVYVNGLFEGNGPVTVRVPRPGKYAVKLDAEGYKTWTQN
                     VVVDANSTFFVKARLLRQEKVVDERIRALQDGRTALLTFATLYSAVASEALLYAFQTE
                     NPRLYIGLPLVAAPLSFFGALKATEGAVMNGGRTFMIVSSTLWGSTWGFAASFVFGAG
                     GGADSGILRPEFAGLSVAGGLLYGAASTYLSAGGEPFPASRAWLFNLGSLLGAFLGLG
                     VPYVLGAESPTVVYVGMLTGSLAGSATALWLTRDFTEGRNIGNLALGSLLEADPGSVS
                     LGLPAPTLTSLPARADGRPALGLYVPLATVRY"
     gene            complement(10910..>11147)
                     /locus_tag="P5313_11160"
     CDS             complement(10910..>11147)
                     /locus_tag="P5313_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80964.1"
                     /translation="VSTMATAGWGSDFVTVTISPLANLAFDVTNSLTFVVQIKSTQDW
                     SDGTTQSNYLDGRDYEGRLWKLDRIVFSYTRKLN"
BASE COUNT         1975 a         3451 c         3625 g         2096 t
ORIGIN      
        1 tcctgggata cctggaacgt gccctgcgtc ccgccgccac ggcctgggac atcatgacag
       61 aggaggtcga ggtcctaggc caggaggaca gcctcctgga cgcgtccctc ttcttcgagc
      121 ggaccatgca caccggagcc cccgtggtgg acgccgaggg gaacctgtcc gggatcatca
      181 ccctgcggga catcctgaag gggcggaagg cggggcagat gaaagccccg gtccggacct
      241 acatgtcccg gaacgtggtg accgcggcgc cggattccac ggtccgccag atcgacgacc
      301 tcttgttccg gcacaacatc ggccacctcc cggtcgtcgt ggaccggaag gtggtgggca
      361 tcgtgacccg gacggacttc ctggaataca agcacaacga gcggcggaag aaggaccagc
      421 tgctcaagga gttccgggaa gaggcctgag cccggccgcc ccgctccggg atcagtggcc
      481 gcccaggtag gcccgcttga cctccgcatt ggccaggagg ttcgcggccg tatccgagag
      541 ggtgatccgt ccggtctcca tgacgtagcc cttgtgggcc gtcttcaagg ccaggttggc
      601 gttctgctcc accaggaaga tcgtcgtctt ctggtcgcgg ttcacctgct cgatgatctt
      661 gaagatctgc tggaccagta tgggggccag gcccagggac ggctcgtcca ggaggagaag
      721 cttgggcctg cccatcaggg cccgggagat ggccagcatc tgctgctcgc ccccggagag
      781 ggtgcctccg gcctggttcc ttcgttccgc cagccggggg aagagttcga agacgtactc
      841 gaaatccttc gcgatcccgg gcttgtcctt ccgcaggtag gcacccatgt ccaggttttc
      901 cttgaccgtg aggcccggga aaatccgcct tccttccggc acctggacga tgccgagctc
      961 cacgatgcgg tcgctggaaa gcccggtgat gtcccggtcc tcgaaaaaga cctttccctc
     1021 ccgggggggc acgacgccgc agacggacat cagggtggtg gacttacccg cgccgttcgc
     1081 gccgatcagg gtgacgatct cgccctgctt gatttccagg gagatcccct tcagggcctg
     1141 gatattcccg taaaaagttg agacggactc gagtttaagc attgacctct tctcccaggt
     1201 atgccttgat cacgacggga ttccgcttga tttcggcggg agtgccctcg gcgatgagct
     1261 ggccgtagtc gactacgtag atccgctcgg aaacccgcat caccaggctc atgtcatgct
     1321 cgatcaggaa gatggagacc ttctcggtgt cgcggatccg cacgatcagg tcgtccaggt
     1381 gtccggtctc ctttgggttc atccccgcgg cgggctcgtc cagcaggagg aggaagggct
     1441 ccgtggcaag ggcccgggcg atctccagga ggcgttgggc gccgtaggga aggttcttgg
     1501 ccagctcgtt cacgtacttt tccagctcga tcttctttaa gatctcgtag gaatcgtgga
     1561 tgaccttctg ttcctcctcc cgggtggatc cgtcccggag gatggcgccc aggatgcctg
     1621 ccttgagccg ggcgtgccgc ccgatcatga cgttctccag gaccgtcatg ttctggaaga
     1681 gccggatgtt ctggaaggtc cgggcaaggc ccttctccgt gaccacgtgg ggcaagagat
     1741 tgttgatgac ctccggggat ttcccgggcg gggttatcga tatggttccc gcggaggggg
     1801 gatacacgcc ggtcacgcag ttgaagaacg tcgtcttccc tgcgccgttc gggccgatca
     1861 gggcgacgat ctccccctgg cgtatggtca tggacacgtc gttcagggcc cgcaggcccc
     1921 cgaaattcat ggtgacgttc ttggcgtcca ggatcgactc actcatgccc ggctccctcg
     1981 acctgtccgt cggccttgaa cacgtagctc ttgcggacct tgcgtatgat tccgcccggt
     2041 cggaagacca tcatgagaac caggatcgat ccataaagta gcatccggta ctgggtgaag
     2101 gcccgcaggt actccggaag aaggatgatg accagggcgc ccacgatcac ccccgctgtg
     2161 gaacccatgc ctcccagcac gatgatgcag aggatcagga cggattccat cagggtgaaa
     2221 ctggcggggt tgatgaaggt cgttttcgcg gcgaagagga ctcccaccat cccggcccag
     2281 aaggcgccca gggcgaaggc cgagagcttg gcgatcgtga ggttcactcc catggcctcg
     2341 gacgccacgg cgtcctcccg catggcctcc caggccctgc ccagccgaga attctccaga
     2401 cggcggacga tgaagaccgt caccaccacc agggccaggg tgatcaggta gaggtagatc
     2461 gcggactggg ggaccgagag cttgatgccg aagaagggag gccggtcgat ctgggagatc
     2521 cccgagggac ccgaggagaa gtcgttccag ttctccagga cgatgcggca gatctccgcg
     2581 aagcccaggg tcacgatggc caggtagtcg cccttgaggc gcaggaccgg gaatcccagg
     2641 atgatcccga agatcgtcgc caggacggcg cccaggggca gggcgatcca gaagctcagg
     2701 ccgaaccggg tgttcaggat cccgtaggta taggcgccga ccgcgtagaa ggccgcgtag
     2761 cccagctgca gaagcccgcc caggccgata acgatgttca gccccaagcc caggattacg
     2821 tacatcaggg ccgtggtgac gatgttcgtc tggtacatcc ccgctacgaa ggggaagagg
     2881 accgctaggg ctaaaaggac ggccagggcc gcccgggaat tctttttctg ggcgaaaacc
     2941 gacttcagct cgatgccccg gagcttggcg ggcacgagcc cggaaaggtc gaagagccgc
     3001 tcgccccggg cccgtttctc cagcagacgt cgccagacgt aggacagggc catggtcagg
     3061 acgaagaccg cggccaggtt gccccagcgc cataggatgg aattccggac cgtgttcacc
     3121 ttgatcacca ggatggggaa ggtgaggaag gcgaaccaga gggcggtcac gaaggatttc
     3181 ttcagttccg gtataagctt gttcatgctc tgccgcctca gatcttttct ttctgggatt
     3241 tgcccagaag cccggagggg cggaaaatca ggatcaggac caggatgatg aaggcgaaca
     3301 cgtcctcgta gtccgaggag atgtacccgg tgccgaagct ttcggtcagg cccagcacga
     3361 agctgccgag cacggcgccc ggtatggaac cgatcccgcc gaggaccgcg gccacgaagg
     3421 ccttgatgcc ggcgatgaag cctatgtaga agttgatctg ccccacgtgg gaactgatca
     3481 ggaccccgcc cacggccgcc atggcggatc cgatgacgaa ggtgaccgcg atgacctggt
     3541 tgatgttgat gcccaccaac agggccatgg tcttgtcctg ggcggtcgcc cgcatggcct
     3601 taccgatctt cgtgaacttg atcagcaggg tcaggagcac catgaccacc gcggtgacca
     3661 ggaagatgat gacctgggtg gagctcagca tccccccgag tccctcgagg aaccggaact
     3721 tggggatgag ccgggggaag gaaacgaagt ccgaagtctg ggccaggagt acgtagtttt
     3781 ggaggaaata cgacatgccg atggcgctga tcagggcgga gaggcggggg gcgttccgga
     3841 ggggcttgta ggcgatcttc tccatggtga agccgtaggc cgcggaatac acgaccgcga
     3901 cgaccatcgc gatggcgaac acggcgatgc cgggcatgcc ggtcatgttg aggaccgtgg
     3961 ccacgatcag ggccgtgaac gccccgatca tgtagatctc cccgtgggcg aaattgatga
     4021 gctggacgat gccgtacacc atcgtatagc ccagggcgat cagggcgtag atgctgcctc
     4081 gggtcatgcc cgagatgaat aactgaagca agtagtttag gtccatgacg gttcctaaac
     4141 ctcccctatc ctggatttcg accggtcgtc gcgcattcgg cccccgatcc tcgcacagaa
     4201 accccggtta cgcaaagggg tgcccaggag ccgtacgctc gatggacacc cctgggtacc
     4261 cggcgggtac ggaatgttca cgtccgcggt cttccggagc gccctcgggc gccccggcga
     4321 accacgttcc ttacttgatc tccacgtaaa cgccgttctg gacctggtac accgagaagc
     4381 ctacgccgat ggcgtcgccc ttggcgtcga agctgatctt cccgaggggg gtctcgacga
     4441 actcggagcg gagggccttg gtgaccgcgt cgtagtccac ggaaccggcc ttctggatgg
     4501 cgttcaggag ggcctgggtg gcggcgtagg cgttcaggaa gaaggctccc ggatcctcgc
     4561 cgtacttctt cttgtgatcc tccgtggcct tgacggccag ggggttcttg gtggtgtcca
     4621 cggggccgga ggcgtagacg ccctcggcgg cttccttggc caccttgatg aaggtgtcgt
     4681 ccttgacgcc gtcgtcggag atgaagacgg tcttcatgcc cttggcgcgc atctggctga
     4741 ccagcttgga ggcctcgggg tggtacccgc cgtacaggat ggcctcggcg ccggaagcgg
     4801 cgaccttgtt gatgatggcg gagtagtcga cggctccgac ctggatgcct tcgaagacca
     4861 ggacctcggc gcccgcggcg tcgagatagc ccttggcgaa ctcggcgaag cccttgccgt
     4921 agtcaccctt gtcgtggagg acggcgaact tcttgaaccc gagcttcttc atggcgaact
     4981 cgacatccac ttccgcctgg gcgtcgtccg acgcgatggt ccggaagaag ttggggtaat
     5041 cgccgctctt ggtcaggggg ggattcgtgg cggacgggga gatcgtgatg atcttggcgt
     5101 ccttgtagat gcccagggcc gccttggtgg ctccggagca gatgtgtccg atgaccgcga
     5161 tgaccttgtc gcctaccagc ttggaagcgg tgttggtcgc cacttcgggc ttgcagacgt
     5221 cgtcggccac gaccagttcg atcttcttgc ccaggacgcc gcccttggca ttccactcct
     5281 ccacgaccag ctcggcggcc ttgaccgacg gcagtccgta ggaggcgagg tcgccggtgt
     5341 gggcgcccgc gacgccgatc ttgatcgtct tcggtccctt gtcggcgcag ccgacgaagg
     5401 ccaggagggc gatcgccgcg agagcggtta ggattaccag cctcttcttc atgttctcac
     5461 tccttgtgca tggggattct tgggagagtt ggacaataat ggaatccttt caacaaataa
     5521 tcaagactct ccccatagaa tcgaaaaaaa aatcgagtgt ccaagaatga gattccttcg
     5581 gtgtgtttcg actgataaat ccaggattcg ggggttgaaa attcacgtgg tttccagtcg
     5641 gccggaagcg gccggatcct gcccaagggt gtccgccagg aagggcaggg cggagcggag
     5701 ggcggattcg aggggccagg gagggttcac gacgaatatc ccgctgcccg ccagaccgcg
     5761 gtcccccgga ccaatgtccc ggacccgaag ctccgcggaa agcttctgtc gagtcgtcaa
     5821 ggccgaaaga cgggccggaa gggcccgggc ttcgggacgc tccaggaggg gataccagac
     5881 catgtaggtt cccgtctcga accttcccag ggcctccgcc agggcctcca cgacccggtc
     5941 gtagtccgag gccagttcgt agggggggtc cagtatgacc agaccccttc gggacggggg
     6001 tggaaggacc gcccgtacgg aggcgaatcc gtcggtcccc tggacgtcga cgcgaccgtc
     6061 cccggcgaac cggatccgaa gctcctcgag atccgccgga tggatttccc tcaacgacag
     6121 ccggtccccg ggacggagca gggcggcggc cagggccggg gaacccgggt actccccggg
     6181 atggattccg ccgtattcct gtatgaactc gaggtactcg gcgacagctt ccggcgggac
     6241 cggggccgtc gcttccccgg aaaggcgttt tatgccggat tcccattccc gggtttgggc
     6301 ggcgtacccc tcctccaggc gatacgcacc ggccccggcg tgtgtgtcca ggtaaaggag
     6361 gggtttgggc ttcttcgtca tgtaccggag gcagtggtac aggactaggt gcttcaggat
     6421 gtcggcgtga ttcccggcgt ggaaggcgtg gcggtagccc agcatggaac ctccgttccc
     6481 ggacatcata ctccgtcacg ggcgtgacgg aaagaccgaa ggcttggcga cggagttccg
     6541 gttcctgtag aatcctcacc gatggctgcc gtcggatttc atcatcgccg atcctccggc
     6601 attcgaggga ctctctcccg ttggtacggc tcccgggtcg gcatattcct ccagagcata
     6661 ctcatcggag tcctcggagg gttcacggtg gtgctcttcc gtctgctcct ggaggaggcg
     6721 gaccgattcc gggcggcggc ttatctcttc cttcgcgggg cgggacccgg atatatcgcc
     6781 gcctggatcg ccgtcctgat cgccggaggt ctcttactgg gctgggcctg cggccgatgg
     6841 cccatgatcc gggggagcgg gatcccccag atcaaggggg ccctcctgcg caagatgcga
     6901 ctggagtggc tccccgaact ccctctcaag atcttgaccg gggtcctcgg catcggcctc
     6961 ggactctccc tggggcggga ggggccttcc gtccagatcg gcgcctatgt aggcaaggga
     7021 gtcctgggag tggcccggcg gcccgtggtc gagcgaaagt acctgatcac cagcggcgcc
     7081 gcagcgggac tcgccgcggc cttcaacgcg cccctggcgg gcgtgctgtt cgccctggag
     7141 gagctgcaca agcacttttc gcccctgctg ctggcctgcg ccatgggcgc ctccgtgacc
     7201 ggggacttcg ttgccagccg tttcttcggc ctggggcccg ccttcgattt ccaccagatc
     7261 gttccgctcc ccctgaccaa gctgccctgg gtcctggtct tcggggtgct ctgcgccttc
     7321 ctgggggacc tgttcaaacg gtctttatac gcggctcagg acctgtatcc ggccatccgc
     7381 atacctcccg cggcccggcc ggcgttggca ctcctcgtgt ccgtgccctt gggattcttc
     7441 ctcttcgatg tgaccggggg agggcatgcc ctgatcgaga ggctgtgggg ggaaagcttc
     7501 agcctcggat tcctcctgct gctcctggcg gccaagatcg ccttttccgc cctttcctac
     7561 ggctccggca ccgcgggggg catcttcctg cccctcctgg cctgtggcgc cctggcggga
     7621 aagctcttcg gaaccgtcct ggcggacctg gggtgggcgc tacccggaga ctcggccaac
     7681 ttcatgatcc tgggcatggc cgccctgttc accggagtcg tcaaggctcc cgtcacgggg
     7741 gcggtcctga tcctggagat gagcgggaac ttcaaccact tcgcggggct catggcctgc
     7801 tgcctgaccg ctttcgtgac cgcggacctg atcaagtcga aaccggtcta cgatgtcctc
     7861 ctggaacgcc tcctggcccg caatcccggc gcgttcaagc ccgtcgccgg aaggaaaatc
     7921 gtcctggaga tccccgtggc cgaggggtcc cgtctctccc acaggctcgt gcggaacctg
     7981 ccgtggcccg aggactgtct ggtggtcggc gtggagcggg gcgaggcgga cctcatcccc
     8041 cacgggtcca cggagatcct tccgggcgac cgactccacg tgctttcgga tgaggaggat
     8101 gccggggagg tcaaggagat cctcctgagg atgggggaga gggaagagta gtccgaccga
     8161 ctgtcgcatc gggcccatga caggactcga tccggccgcg ctcaatctat tttgacattt
     8221 acgtcttttt tgttgaatta tttccgaccc gatagtattc tcatcaggaa tccggccgta
     8281 ggccgaatcc acatcgcccc agcaaggagt ccccatgaag aagttcctcg ttcttctcct
     8341 ggttctggcc gccctggcgc cggctttcgc ggatgacgcg aaggtcctgc ccaagggtgt
     8401 cttccggctt tcgttcgtgt tcgcccggaa tgcgtatgat gagcggtatg atgcaaatgg
     8461 ggatatctct tcaacgggtg aggtatccgc cctcgccatc gggacggctc tagaatttgg
     8521 cgtaaccgat caagtcacag ctgccgttca gtggactccg gcttatgcct tcaatacttc
     8581 gatcgaatcc gtagccccgc ttccatttga ccctacgctg gcgaatgcag atggtcttgc
     8641 ggatcttttc gtaggtgcga aattcctgat tatgggtgct cagggatttg ttcctaatga
     8701 tacgttccga atcgcttttg ctcctggtgt attgattcct ctcgacaacc ccgattgggc
     8761 tgctgaagta gcggcagcta taggtggaaa tgaatacaaa gtgaccagtc ctagtactca
     8821 agcgattgga ctcggattcc gcacgtactt cgactgggta ttgagcaaga tgttctacat
     8881 caacctctat aatcagacca ttttttactt acctgtcacg aagtcccagt tcaatccact
     8941 cgataccacc gagcatgagt acgcctacgg ctacaagctc accttcgagg cggaacccca
     9001 cttcgattat tccttctcgg acaaggtttc cgtgagcgtc ggcgtgcccg tgacctttac
     9061 catggggccc cagctggaga tcgacggcgc tccttcggcc acgaatcccg agtattacat
     9121 tctttccgct tccccgagcg cctcggtctt cgtgatggcc ttcatgccct tcgagctcaa
     9181 ggccggctac accctgccga ttctcggcaa gaacgcgaac gccgccagta ccctggtatt
     9241 ccagctcaag accttcctga agttctagcg attcccgccg cgccgactcg gcgcgcgacc
     9301 cgacggcccc gcccagcgcg gggccgttct tattcagtac cgcaccgtcg ccaggggaac
     9361 atagagcccc agggccggcc gcccgtccgc ccgggccggg agggacgtca gggtcggcgc
     9421 gggcaggccc agggatacgg atcccggatc cgcctctaaa agggaaccca gggcgaggtt
     9481 gccgatgttc cgtccctccg tgaagtcccg ggtcagccag agggcggtgg cggagcccgc
     9541 aagggacccg gtcagcatac ccacatacac cacagtcggg gattccgctc ccaggacgta
     9601 gggcactccg aggcccagga aggcccccag gagggatccc aggttgaaga gccaggcccg
     9661 ggaggccggg aagggctctc ccccagcgga caggtaggtg gaggcggcgc cgtacagcag
     9721 gccccctgcc acggagaggc cggcgaactc ggggcggagg atgccggagt ccgcccctcc
     9781 cccggctccg aacacgaagg acgccgcgaa gccccaggta ctgccccata gggtgctgga
     9841 gacgatcatg aaggtgcggc ctccgttcat cacggccccc tcggtggcct tgagggcacc
     9901 gaagaaggac aggggggcgg ccacgagggg caggccgatg tacagccgcg gattttccgt
     9961 ctggaaggcg tacaacaatg cctcggaggc gacggcggag tagagggtgg cgaaggtgag
    10021 cagggcggtg cgaccatcct ggagggcccg gatcctctcg tccacgacct tctcctgccg
    10081 gaggagcctg gccttgacga agaaggtgga gttcgcgtcc acgaccacgt tctgggtcca
    10141 ggtcttgtac ccctcggcgt ccagcttgac cgcatacttc cccggccggg gcacccggac
    10201 cgtcaccggt ccgttcccct cgaagaggcc gttcacgtag accgcgaccc cggcgggctc
    10261 ggtctccacg cggatatatt cgccctgctg gacctcgatc cccgggatgc ggaggatctt
    10321 ctgggcggcg atccggacgg gcttgcggat gtcctcgagg ttgcccatga actcctcggt
    10381 catggcgcgc tccacggtcc cggtctccac gtccacgata cgcagggaaa tgatgtacag
    10441 ggttccgagt atgccgatgc ggccgaccat gagggactgg gcccccgcaa ggcggcctat
    10501 cctggacgcc cccttgggat cgaaggcgtc ggacagcccg agctcctgct cccggagcag
    10561 ggcgtccagg tccgcccgtt ccacgaggga gaatccgggg gcgatggaga tctcccgtcg
    10621 gacgagctcc gacaggatgg cgagtcccga gggtgtgacg tcctcggact ggaaacccag
    10681 gaacacgatg gagcgcgggg aagaagattc ggcgggggcc tgggccgcga tcgccgccgc
    10741 ggatgcggcg gcaaggaagg cgatgaacac ttttttcatc atcttccgcg ggatccgggg
    10801 agggcgggat cccgctcctc cttccaaaag gaatccgggc gtccgcggcc cggacggccg
    10861 agcggacgcc ctccctacag tatagcccgg aagatgccgg ccgtcccgtc taattcagtt
    10921 tccgggtgta gctgaacacg atccggtcca gcttccacag gcggccctcg tagtcgcgac
    10981 catcgaggta gttggactgt gtcgtgccgt cggaccagtc ctgggtgctc ttgatctgca
    11041 cgacgaaggt caggctgttg gtcacgtcga aggccaggtt ggccaggggg ctgatcgtga
    11101 cggtgacgaa gtccgagccc cagcccgcgg tggccatggt cgagacg
//
LOCUS       DAOTEI010000113        11126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4144_length_11126_cov_5.332852, whole genome shotgun sequence.
ACCESSION   DAOTEI010000113 DAOTEI010000000
VERSION     DAOTEI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4144_length_11126_cov_5.332852"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="P5313_11165"
     CDS             complement(<1..1333)
                     /locus_tag="P5313_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRY80965.1"
                     /translation="MNDSESIRKGFATYTEGVEKSLGKFPERKGKFVTGSNAPVDRLY
                     TPLDLEGRDYLTDQGFPGTYPFTRGVQNTMYRGRFWTMRQYAGFATAEESNKRYKYLL
                     EQGQTGLSVAFDLPTQIGYDSDHALSQGEVGKVGVAIDSLADMEILFDGIPLDKVSTS
                     MTINAPASVLLAMYIAVGEKQGVTPDKLNGTIQNDILKEYVARGTYIFPPAPSMRLIT
                     DIFEYCSKEVPNWNTISISGYHIREAGSTAVQEVAFTLADGVAYVEAAVKAGLDVDEF
                     APRLSFFFNAHNDLFEEVAKFRAARRVWAKIMKDRFGAKNPKSWMLRFHTQTAGSTLT
                     AQQPDNNVVRVTLQALAAVLGGTQSLHTNSKDEALALPTEASVRVALRTQQIIAYESG
                     AAETADPLAGSYYVESLTNRIEDGAWDYIRKIDSLGGAVKAIENGYVQQEIK"
     gene            complement(1368..1907)
                     /locus_tag="P5313_11170"
     CDS             complement(1368..1907)
                     /locus_tag="P5313_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRY80966.1"
                     /translation="MRTEFRLSGSGGQGLLLAGIVLAEAAILEGKNAVQTQSYGPEAR
                     GGASKAEVVISGEDIDYPKAMDPDFLLALTAEAYRTYGKLMKKGLIIVDESVPPSEDI
                     IARTARVPILKTAAKDIGKSVVANIVALGVLAGVSGIVEPGTLEAAVKNRVPKGTEEL
                     NVAALKKGFELATAFKAKA"
     gene            complement(1886..2746)
                     /locus_tag="P5313_11175"
     CDS             complement(1886..2746)
                     /locus_tag="P5313_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRY80967.1"
                     /translation="MSDILKYFRQDRLPHIWCPGCGHGTVTGALVRAIARLKLDKNKV
                     CVVSGIGCSSRAPGYLDFDTLHTAHGRALAFATGIKLARPDLKVIVMTGDGDCTAIGG
                     NHFIHAARRNIDITTIVMNNNIYGMTSGQYSPMTPKGFLGTTAPYGNPERTFDLCKLA
                     EAAGATYVARGTSFHAQMLIDLIEQALTHRGFSVVEALTQCPTYFGRKNKVGGAVELL
                     EWIKERTVNAKAAAVLPPEKVAGKLLIGELHKNPQPEFCDEYDKIIAQVQRKFEGFRG
                     GVRGCEQNSD"
     gene            complement(2743..3876)
                     /locus_tag="P5313_11180"
     CDS             complement(2743..3876)
                     /locus_tag="P5313_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRY80968.1"
                     /translation="MPSAKLMQGNEACTIGALVAGLEFYAGYPITPSTEIAELCSEEL
                     PKVGGKFVQMEDEIGSIAAIIGASLTGKKSMTATSGPGFSLMQESLGYASETEVPVVI
                     INVQRMGPSTGMPTSPAQGDVMQARWGTHGDHPVIALAPGNVKECFDLTVRAFNLAEE
                     YRVPVIVLTDEVIGHMREKVLLPDWEDVKVVDRARPTAPGGYRPYEADLDGGVPLMAP
                     FGQGYRWHVTGLFHDETGFPSGKADVADRLLRRLDRKITKNEKVIRDYVTESVEDCEI
                     LVVSFGSSAMSALSAVRRARREGVKAGMLRLKTIWPFPDEVLKDLSRNAKAVLVPEMN
                     LGQLAYEVERTVGCRAPVVRIGKVNGDLFRPDEIHDKIMEAAK"
     gene            complement(3869..4138)
                     /locus_tag="P5313_11185"
     CDS             complement(3869..4138)
                     /locus_tag="P5313_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY80969.1"
                     /translation="MSEKKSYEHVIREKWCKGCNICVAYCPKKVLTLVNGKVFAERPH
                     DCIGCRLCELRCPDFAIEIREATGAPEPEEETTVDYTIPPEATHA"
     gene            4426..5355
                     /locus_tag="P5313_11190"
     CDS             4426..5355
                     /locus_tag="P5313_11190"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY80970.1"
                     /translation="MTLQQLRYFQVLAKIEHFTKASQALCITQPSLSYAMSELEKELD
                     VQLFDKHGKKIALSAQGRKFLKFVDGSLALLDEGIETVRLSGPVQGRLTLGYISSLSS
                     TFLPEVLTSFYKDPANEKITFNFVQNLNNALIEGLKQGDIDLAFCPDPSKDVASAPVL
                     RQELFLVIPRSHRLAQRPEIDIHEVKDEPFILTNRKSGLRRMIGNIFREMKIRPKVAF
                     EAEDCTVAITFVSLQYGLSIIPVTPNLGNPDVVGVRLKNPEFSRTVYMAWTPSRNLAP
                     AVRRVRDFIAERWPSGVGASASDTRIAYPEISL"
     gene            5404..7089
                     /locus_tag="P5313_11195"
     CDS             5404..7089
                     /locus_tag="P5313_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HRY80971.1"
                     /translation="MNKALKLRDLTLRDGQQSQFATRMTQAQVDRVLPHFRKAGFYAM
                     EVWGGAVPDSIMRYLGESPWARLESIKAGIAGASKLTALSRGRNLFGYTPYPDSVIEG
                     FCRNSVRSGIDIMRIFDALNDISNMTGTLRYVKENGGLADCAICYTVDPRFTRMDRFK
                     AFLSGRKLPGSLFNIPYYVARAKELEGLGADMISIKDMAGLINPATAGHLIRALKDAV
                     SIPVDLHTHCTPGFGTASLLMGMANGVDIVDTVVLPFSGGPAAPAYEIIQIFADRMGL
                     DTGVDRAAVQAISDELRAIRLDLAKFDQYKRMPPAFDIRNPELPPDVSRLFDDAIEAA
                     RADRPQAVLDLCQRIEAFFNYPEPDNVVRVAQIPGGMYTNMMDQLKEAKLEQHLQEVL
                     AIVPKVRLDAGVPPLVTPTSQIVGVQAVACVVDKTQGKPFYSNVSKNFAELVKGTYGK
                     TPWPVDPEFRLKIAGVREETPYDTSAYRRQDNPELPEFGGTRLAKDEKEELLLELFPS
                     VADRYLRSVRAREWEEWQAAHPPAPEPEIPNGPWPADFWERDLSAYAGFVTTS"
     gene            complement(7079..8011)
                     /locus_tag="P5313_11200"
     CDS             complement(7079..8011)
                     /locus_tag="P5313_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY80972.1"
                     /translation="MASEMILLVTAALWGFAFVAQRVGMDFMGPLTYNGIRFLMGGAS
                     LLPLIYLRRKLDRGRGLRAPEGSGAARPAPGGARTLAVSSLAGGLIITVAANLQQWSM
                     KFITAGKAGFITGLYVVLVPIAGMLLKRKTPVLAWAGCAAAAGGLYFLSVTEEFTIAR
                     GDLIVLASTFFWTCHILFLDTFSKRVDPVELSAGQFLVCGALTSAGALAFEAPSWESV
                     RAAAVPLLYGGLVSIGIAYTLQAVAQRKAHPTRAAIVMSLETVFAAAGGALLLSERLS
                     PRELLGCALMFAGMLIAQAPGPKPKAAAESAIRR"
     gene            complement(8083..9168)
                     /locus_tag="P5313_11205"
     CDS             complement(8083..9168)
                     /locus_tag="P5313_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRY80973.1"
                     /translation="MIIREPGPQTTVQDLGRFGSQSLGVPPSGAADPDALETANLLVG
                     NNPGEACLECTLRGPVLEFTGPAAFSLCGGRSEARLNDAEVPLWATVYAKAGDVLTIG
                     PVETGLRIYLSVGGGLDIPPVLGSRSTYARAGFGGWEGRALQAGDRIPLRTDPDPRPL
                     PAWIVPEGERPGYPSEIRVRAVPSHEIDRFRPESVERFFRDPYAVSPKSDRMGCRLEG
                     TPLVHVRGADIVSSGVLTGTVQVPGDGLPIVLLADRQTTGGYTRIAHVIRTDLPKLGQ
                     ARPGDRVRFDRATLEEAREAWDAREAVLRSRIRPRATVLPGAAGDSAAALQSTSPGAP
                     DRAFVGPRRRFRVVVDGREYDVELLEV"
     gene            complement(9174..9908)
                     /gene="pxpB"
                     /locus_tag="P5313_11210"
     CDS             complement(9174..9908)
                     /gene="pxpB"
                     /locus_tag="P5313_11210"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRY80974.1"
                     /translation="MSDSLTVHPYGETALTAVFGDGISEDLYRRIRALTSALEADPPR
                     GLSELIPAYNSLLVVYDPREAAYEDLAVIVRDRAARSAELAPGPSRVARIPVCYEPPH
                     APDLEDVARHAGLSPEEVVSIHSAGAYLVYMLGFTPGFPYLGGLDDRIAAPRRATPRT
                     RIPAGSVGIADRQTGIYPQASPGGWNLIGRTPAILFDPARTPPALLAAGMYIRFTPIS
                     AAEFEDLSEAAASDRWRPEIAEEDRP"
     gene            complement(9905..10678)
                     /locus_tag="P5313_11215"
     CDS             complement(9905..10678)
                     /locus_tag="P5313_11215"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRY80975.1"
                     /translation="MEIDLNADVGEGFGAYALGNDEALLGLVSSANIACGFHAGDPGV
                     MERTVGRALRGGAALGAHPGYPDLQGFGRRSLALSENEVYAAVLYQIGALRAFARAAG
                     SDLRHVKPHGALYNDAARNPALARGAARAVKAAGGNLILVGLPGCELEAAAVEAGLPY
                     AREFFADRAYQDDGSLVPRSRPDAVIHDEDACVERVLRAVRDGTVVTATGKSIPVRAD
                     TVCLHGDNPEAVAFARRLRSALQAAGISIRPVAPGDARS"
     gene            complement(10663..>11126)
                     /locus_tag="P5313_11220"
     CDS             complement(10663..>11126)
                     /locus_tag="P5313_11220"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HRY80976.1"
                     /translation="ADRLFSPDPGRGWVTLAVGDGGNELGMGGLRETLKAEVSRGDLI
                     FCASPADHTLACGISNWGGYALAAALSLLSGVLLLRTPDQERALLTALVDAGAVDGCT
                     REKGLSVDGLAWEKYAETLHAMYDLASGALSARKRGPEAGIGDGGGPWKST"
BASE COUNT         1790 a         3782 c         3666 g         1888 t
ORIGIN      
        1 ccttgatctc ctgctggacg tatccgttct cgatggcctt gacggccccg cccagggagt
       61 cgatcttgcg gatgtagtcc caggcaccgt cctcgatgcg gttcgtgagg ctctccacgt
      121 agtaggagcc cgccaggggg tccgcggtct ccgcggcgcc gctctcgtag gcgatgatct
      181 gctgggtacg gagggccacc cggacggatg cctccgtggg cagggccagg gcctcgtcct
      241 tggagttggt gtggagggac tgggtcccgc ccagcaccgc ggccagggcc tggagggtca
      301 cccggaccac gttgttgtcc ggctgctggg ccgttagagt ggaacccgcg gtctgggtat
      361 ggaagcggag catccagctc ttgggattct tggccccgaa gcggtccttc atgatcttgg
      421 cccagacccg gcgggcggcc cggaacttgg cgacctcctc gaagaggtcg ttgtgggcgt
      481 tgaagaagaa ggagagcctc ggggcgaact cgtccacgtc cagccccgcc ttgaccgcgg
      541 cctccacgta ggcgaccccg tcggccaggg tgaaggcgac ttcctgcacc gccgtggaac
      601 cggcttcccg gatgtggtag cccgagatgg agatggtgtt ccaattcggg acttccttgg
      661 agcagtactc gaagatgtcc gtgatcaggc gcatggacgg ggcgggcggg aagatatagg
      721 taccccgggc cacgtattcc ttgaggatgt cgttctggat ggtgccgttc agcttgtccg
      781 gggtgacccc ctgcttctcc cccacggcta tgtacatggc caggagcacc gacgcggggg
      841 cgttgatggt catcgaggtg gaaaccttgt ccaggggaat cccgtcgaag aggatctcca
      901 tgtccgcgag ggagtcgatg gccaccccga ccttcccgac ctcgccctgg gacagggcgt
      961 ggtcggagtc gtacccgatc tgggtgggga ggtcgaaggc cacggagagc cccgtctggc
     1021 cctgctccag caggtacttg taccgcttgt tggattcctc cgcggtggcg aaacccgcgt
     1081 actggcgcat ggtccagaag cggccccggt acatggtgtt ctggacgccc cgggtgaagg
     1141 gataagtgcc cgggaagccc tggtccgtca ggtagtcccg gccttccaag tccagggggg
     1201 tgtagagccg atccaccggg gcgttggacc cggtgacgaa tttgcccttc cgctccggga
     1261 acttgcccag ggatttctcc accccctccg tgtatgtcgc gaatcccttg cggatcgatt
     1321 cggaatcgtt catctcatat cctccggtat gtgcggcggc gcgggcttca ggccttggcc
     1381 ttgaacgcgg tggccagctc gaatcccttc ttcagggccg cgacgttcaa ctcctcggtg
     1441 cccttgggaa cccggttctt gaccgcggct tccagggtcc cgggttccac gatcccggag
     1501 actccggcca ggacccccag ggccacgatg ttggccacca cgctcttgcc gatgtccttg
     1561 gccgcggtct tgaggatcgg cacccgggcc gtccgtgcga tgatgtcctc ggacgggggt
     1621 acggactcgt ccacgatgat gagccctttc ttcatgagct tgccgtaggt cctgtaggcc
     1681 tctgcggtca gggccagcag gaagtcggga tccatcgcct tggggtagtc gatgtcctcg
     1741 ccgctgatca ccacttccgc cttggaggcc ccgccccggg cctcgggccc gtagctctgg
     1801 gtctgcacgg cgttcttgcc ttccaggatg gccgcctcgg caaggactat tcccgcgagg
     1861 agcaggccct gccccccgga tccgctcagt cggaattctg ttcgcatccg cgcacccctc
     1921 cccggaaacc ttcgaacttt cgctgcacct gggcgatgat cttgtcgtac tcgtcgcaga
     1981 actcgggctg gggattcttg tgcagttccc cgatcagcag cttgcccgcg accttctcgg
     2041 gcggcaggac cgccgcggcc ttggcgttga cggtccgctc cttgatccac tccaggagct
     2101 ccaccgcccc gcccaccttg ttcttccgcc cgaaataggt cgggcactgg gtcagggcct
     2161 ccaccaccga gaagccccgg tgcgtcaggg cctgctcgat caggtcgatg agcatctggg
     2221 cgtggaagga tgtgccccgg gcgacgtagg tggccccggc ggcctcggcc agcttgcaca
     2281 ggtcgaaggt ccgctccgga ttcccgtagg gggccgtggt gcccaggaag cccttggggg
     2341 tcatggggga atactggccg ctggtcatgc cgtagatgtt gttgttcatc acgatggtgg
     2401 tgatgtcgat gttccggcgt gccgcgtgga tgaagtggtt gcctccgatg gccgtgcagt
     2461 ccccgtcccc ggtcatcacg atgaccttca ggtccggccg ggccagcttg atccccgtgg
     2521 cgaaggccag ggcgcggccg tgggcggtat gcagggtgtc gaaatccagg tagcccgggg
     2581 cccggctgga gcagccgata ccggagacca cgcagacctt gttcttgtcc agcttgaggc
     2641 gggcgatggc ccgcaccagg gctccggtca ccgttccgtg cccgcagccg gggcaccaga
     2701 tgtgcgggag cctgtcctgt cggaagtact tgagtatgtc gctcatttgg ccgcttccat
     2761 gatcttgtcg tggatctcgt ccgggcggaa gaggtccccg ttcaccttgc cgatgcgcac
     2821 cacgggggct cggcagccta cggtccgctc gacctcgtag gcgagctgtc ccaggttcat
     2881 ctccgggacc agcaccgcct tggcgttcct ggacaggtcc ttgaggacct cgtccgggaa
     2941 gggccatatg gtcttgagcc ggagcatacc ggccttgacg ccttcccgcc gggctcgccg
     3001 gaccgcggac agggcgctca tggcggaact cccgaaggag acgaccagga tctcgcaatc
     3061 ctcgacggac tctgtgacgt aatcccggat caccttctcg ttcttcgtga tcttccggtc
     3121 caggcgccgc aggagccggt ccgccacgtc ggccttgccc gagggaaagc cggtctcgtc
     3181 gtggaagagc cccgtcacgt gccagcggta cccctgtccg aagggggcca tgagaggcac
     3241 cccgccgtcc aggtccgcct cgtaggggcg gtagccgccg ggagccgtgg ggcgggcccg
     3301 atccacgacc ttcacgtcct cccaatccgg gaggagcacc ttttcacgca tgtggccgat
     3361 cacctcgtcc gtgaggacga tcacgggcac gcggtactcc tcggccaggt tgaaggcccg
     3421 gaccgtgagg tcgaagcact ccttcacgtt gcccggagcc agggcgatga cgggatggtc
     3481 gccgtgggtt ccccagcggg cctgcatgac gtcgccctgg gcgggggacg tgggcatacc
     3541 ggtggagggt cccatgcgct ggacatttat gatgaccacg ggtacctcgg tctccgaggc
     3601 gtaccccagg ctttcctgca tcagggagaa gcccgggccg ctggtcgcgg tcatggactt
     3661 ctttcccgtc agggacgccc cgatgatggc cgcgatggag ccgatctcgt cctccatctg
     3721 gacgaacttg cccccgacct tgggaagctc ctcggagcag agctccgcga tctccgtgga
     3781 gggagtgatc ggatatcccg cgtaaaactc gagccccgct accaaggctc cgatcgtgca
     3841 ggcctcgttc ccctgcatca gctttgcgct aggcatgggt ggcctccggc gggatcgtgt
     3901 aatccacggt ggtttcctcc tcaggctccg gggctccggt agcctcgcgg atctcgatgg
     3961 cgaaatccgg acagcggagc tcgcagaggc ggcacccgat gcagtcgtgg ggccgttcgg
     4021 cgaagacctt gccgttcacc agtgtgagga cctttttagg gcagtaggcc acgcagatgt
     4081 tgcagccctt gcaccatttc tctcggatga cgtgctcgta ggatttcttt tcgctcatgg
     4141 ctctccacgc ttctcgtggt atcgttgcag cgccgcccca gcggcgcgga aggcgtttct
     4201 cggcgtatcg cggagtacgc ggtttccagg ggcttcctgt cttttgcggt tccttgagga
     4261 tataccggtt gaccattcct gtaaagcgcg cccggacgag gtctgccccg catcatccta
     4321 caccgggtcc acggataggt ctaataaaat tttttatact gaaaccatag accctttcta
     4381 tgattcccgg tatactcggc cctgccggcg ggaaggacgg cgcacatgac cctccagcag
     4441 cttcgctatt tccaggtcct ggccaagatc gagcacttca ccaaggcctc ccaggccctg
     4501 tgcatcaccc aaccctccct gagctatgcg atgtcggagc tcgagaagga actggatgtt
     4561 caactattcg acaagcacgg gaagaagatc gccctgagcg cccagggccg gaaattcctc
     4621 aaattcgtgg acggttccct ggccctgctg gacgaaggca tcgagaccgt ccggctttcc
     4681 ggccccgtgc agggcaggct aaccctgggg tacatatcca gcctgagctc caccttcctg
     4741 ccggaggtcc tcaccagctt ctacaaggac cccgcgaacg agaagataac cttcaacttc
     4801 gtgcagaacc tgaacaacgc cctgatcgaa ggactcaagc agggcgacat cgacctggcc
     4861 ttctgcccgg acccgtccaa ggacgtggcc tccgccccgg tattgcggca ggaactcttc
     4921 ctggtcatcc cccggagcca ccggctggcc cagcgtccgg agatcgacat ccacgaggtc
     4981 aaggacgagc ccttcatcct gaccaaccgc aagagcgggc tcaggcgcat gatcgggaac
     5041 atcttccggg agatgaagat ccggcccaag gtcgccttcg aggcggagga ctgcaccgtc
     5101 gctatcacct tcgtgtccct gcagtacgga ctttccatca tccccgtgac cccgaacctc
     5161 ggcaatccgg acgtggtcgg cgtccgcctc aagaacccgg aattttcccg gaccgtctac
     5221 atggcctgga cgccctcccg gaacctggcc cccgcggtcc gcagggtccg ggacttcatc
     5281 gcggagcgat ggccttccgg cgtcggggcc tccgcctccg ataccaggat tgcctatccc
     5341 gaaatttctc tatagactat tcggggagta agatttccct ttcttcattg caggaggacg
     5401 cccatgaaca aggcgctgaa gctccgggac ctgactctcc gggacggcca gcaatctcaa
     5461 ttcgccaccc gcatgaccca ggcccaggtg gaccgggtcc tgccacactt ccgcaaggcc
     5521 ggtttctacg ccatggaggt atggggaggg gcggttccag actccatcat gcgctacctg
     5581 ggcgaaagcc cctgggcacg gctggaatcc atcaaggcgg ggatcgcggg cgcttccaag
     5641 ctgaccgccc tctcccgggg tcggaacctc ttcggctaca ccccctatcc ggattccgtc
     5701 atcgagggat tctgccgcaa ctccgtgcgc tccggcatcg acatcatgcg tatcttcgac
     5761 gccctgaacg acatctccaa catgaccggc accctgcggt acgtcaagga gaacgggggc
     5821 ctggcggact gcgccatctg ttacaccgtg gatccccgct tcacccggat ggaccgcttc
     5881 aaggccttcc tctccggcag gaagctcccc ggctcgctgt tcaacatccc ctactacgtg
     5941 gcccgggcca aggaactgga aggcctcggg gcggacatga tctccatcaa ggacatggcg
     6001 ggcctcatca acccggccac ggcgggccac ctgatccggg ccctgaagga cgccgtttcc
     6061 attcccgtcg acctccacac gcactgcacc ccgggcttcg gcaccgccag cctcctcatg
     6121 ggcatggcca acggggtgga catcgtggac accgtggtcc tgcccttctc gggcggcccc
     6181 gccgccccgg cctacgagat catccagatc ttcgcggacc gaatgggcct ggacaccggg
     6241 gtcgaccgcg ccgcggtcca ggctatcagc gacgagctcc gggccatccg gctggacctg
     6301 gccaagttcg accagtacaa gcggatgccc ccggccttcg acatccggaa tccggagctc
     6361 ccccccgacg tgtcccggct cttcgacgac gcgatcgagg cggcccgggc ggaccggccc
     6421 caggccgtcc tggacctctg ccagcggatc gaggccttct tcaactaccc cgagccggac
     6481 aacgtcgtgc gggtcgccca gatccccggc gggatgtaca ccaacatgat ggaccagctg
     6541 aaggaagcca agctggaaca gcacctccag gaggtgctcg ccatcgtccc caaggtccgg
     6601 ctggacgccg gggttcctcc cctggtcacc cccaccagcc agatcgtggg cgtccaggct
     6661 gttgcctgcg tggtcgacaa gacccagggc aagcccttct actccaacgt atcgaagaac
     6721 ttcgcggaat tggtgaaggg gacctacgga aagaccccct ggcccgtgga cccggagttc
     6781 cggctcaaga tcgccggagt ccgcgaggag accccttacg acacctccgc gtaccggagg
     6841 caggacaacc ccgagctccc ggaattcggg ggcacccgcc tggccaagga cgagaaggag
     6901 gagctgctcc tcgaactttt ccccagcgtg gcggaccggt acctgaggag cgtccgcgcc
     6961 cgggaatggg aggaatggca ggcagcccat ccccccgctc cggagccaga gatcccgaac
     7021 ggcccctggc ccgcggattt ctgggagcgg gacctttcgg cctacgcggg cttcgtgact
     7081 acctcctgat cgcggactcg gcggccgcct tcggtttcgg accgggggcc tgggcgatca
     7141 gcatgcccgc gaacatgagg gcgcagccca ggagctcccg gggggaaagc ctctcggaca
     7201 acaacagcgc ccctcccgcc gcggcgaaca cggtctccag gctcatgacg atcgcggccc
     7261 gggtgggatg ggccttcctc tgggccacgg cctgcagggt gtaggcgatt ccgatggaga
     7321 ccagtcctcc gtacagcaga gggaccgccg cggcccggac actttcccag gaaggcgcct
     7381 cgaaagccag ggccccggcc gaggtgaggg cgccgcagac caggaactgg cctgcggaga
     7441 gctcgacggg gtccaccctc ttggagaagg tatccaggaa gagtatgtgg caggtccaaa
     7501 agaaggtgct ggccaggacg atcaggtccc cccgggcgat ggtgaactcc tcggtcacgg
     7561 acaggaaata gagccccccg gccgcggccg cgcatcccgc ccaggccagg accggggttt
     7621 tccgtttcag cagcattccc gcgatcggta ccaggaccac gtacagcccg gtgatgaacc
     7681 ccgcctttcc cgcggtgatg aacttcatgg accactgctg gaggttggcg gccacggtga
     7741 tgatgagacc tcccgcgagg ctggagaccg cgagggtccg tgccccgccc ggcgcgggcc
     7801 gcgccgcccc ggaaccctcg ggcgcgcgga gccccctacc ccgatccaac ttcctccgga
     7861 gatagatcag gggcaagagg gaggctccgc ccatcaggaa gcggatcccg ttgtaggtca
     7921 ggggccccat gaagtccatg ccgacccgct gggccacgaa ggcgaagccc cagagggcgg
     7981 cggttaccag aaggatcatt tccgaggcca gggatcgggt ttccataggg ttcctttagt
     8041 ttcgattata tccgaggttc acggaccgga gtccggaaag cgtcagactt ccagcaactc
     8101 gacgtcgtat tcccgtccgt ccacgacgac ccggaaccga cgccgcggac cgacgaaagc
     8161 ccggtccgga gcacccgggc tcgtcgactg cagggcggcg gctgagtctc cggcggcccc
     8221 ggggagcacg gtcgcccggg gtcggatccg ggaccggagg accgcttccc gggcatccca
     8281 ggcttcccgg gcttcctcca gggtcgcgcg gtcaaagcgg acgcggtcgc ccgggcgggc
     8341 ctggcccagc tttggaaggt ccgtccggat cacgtgggcg atccgggtat agccgccggt
     8401 ggtctgccgg tccgccagga gtacgatggg aaggccgtcc ccgggaacct ggaccgtacc
     8461 ggtcagcacc ccggatgata cgatgtcggc tccccggacg tggaccaggg gggtcccttc
     8521 cagccggcag cccatacggt cgctcttcgg ggacaccgcg tagggatccc ggaaaaaccg
     8581 ctccacggac tccggccgga accggtcgat ctcgtgggac ggcacggcgc ggacccggat
     8641 ctccgaaggg tatcccggcc gctcgccctc cggtacgatc cacgcgggca gaggccgagg
     8701 gtccggatcc gtgcgcaggg gtatgcggtc cccggcctga agggccctgc cctcccagcc
     8761 cccgaagccg gcccgggcgt aggtggagcg gcttcccagg accggaggta tatccagccc
     8821 tccccccacg gagaggtaga ttcgcagtcc cgtttccaca gggccgatcg tgaggacatc
     8881 cccggcctta gcgtataccg tcgcccagag ggggacctcg gcgtcgttca gtcgagcctc
     8941 cgaacggcct ccgcagaggc tgaaggccgc aggccccgtg aactccagca cgggcccccg
     9001 gagggtgcac tccaggcagg cttcgcccgg attgttgccc accagaaggt tcgcggtttc
     9061 cagggcgtcc gggtccgcgg ccccggaggg agggaccccc agggactggc tgccgaagcg
     9121 gcccaggtcc tggaccgtgg tctgggggcc gggctcgcgg atgatcatct ccgtcacggc
     9181 cgatcctcct cggcgatctc gggcctccat cggtccgagg cggcggcctc ggacaggtcc
     9241 tcgaactccg cggcggatat cggggtgaac cggatgtaca tccccgcggc caggagggcc
     9301 gggggagtcc gggccggatc gaagaggatc gcgggggtgc ggcctatgag gttccacccc
     9361 ccggggctgg cctggggata gatcccggtc tgccgatccg ctatgcccac ggatcccgcg
     9421 gggattcgcg tccggggggt ggcccgcctt ggagcggcga tccgatcgtc caggcccccc
     9481 aggtacggaa atcccggggt gaacccgagc atgtatacga ggtaggcgcc ggcggagtgg
     9541 atggaaacga cctcctccgg ggacaacccc gcgtgccggg ccacgtcctc gaggtccggg
     9601 gcgtggggcg gttcgtagca gaccgggatc cgtgcgacac gggacggccc cggggctagc
     9661 tcggcggatc tggcggcccg gtcccggacg atcaccgcca agtcctcata ggcggcctct
     9721 cgggggtcgt agaccacaag cagggaattg taggccggaa tgagctccga aaggccccgg
     9781 ggcgggtcgg cctcgagggc ggacgttaaa gcccggatcc ggcggtagag gtcttccgag
     9841 atcccgtccc cgaacacggc ggtcagggcg gtctccccgt aggggtgcac agtcagggaa
     9901 tcgctcacga gcgtgcgtct cccggagcca cgggccggat cgagatcccc gcggcctgga
     9961 gggccgaccg gagcctgcgc gcgaaggcta ccgcctcggg gttgtccccg tgcaggcaga
    10021 ccgtgtccgc ccggacgggg atggacttcc ccgtcgcggt taccacggta ccgtcccgca
    10081 ccgcccgcag gacccgctcc acgcaggcgt cctcgtcgtg gatcaccgcg tccggccggg
    10141 accgggggac cagggaaccg tcgtcctggt aagccctgtc cgcgaagaac tcccgggcgt
    10201 agggaagccc cgcctcgacg gcggccgcct ccagttcgca gccgggaagc cccaccagga
    10261 tcagattccc gcccgcggcc ttgaccgccc gggccgctcc ccgtgcgagg gcgggattcc
    10321 gggccgcgtc gttgtagagg gctccgtggg gcttgacgtg ccggaggtcc gagcccgccg
    10381 cccgggcaaa ggcccggagc gccccgatct ggtagaggac ggccgcgtag acctcgttct
    10441 cactcagggc cagggacctg cgtccgaagc cctggaggtc cgggtagccg ggatgggccc
    10501 ccagggccgc ccctccccgg agggcccggc ccacggtccg ctccatcacg ccgggatccc
    10561 cggcgtggaa gccgcaggct atgttcgccg agctcaccag tcccaggagg gcctcgtcgt
    10621 ttcccagggc gtaggcgccg aaaccctcgc ccacgtcggc gttcaggtcg atttccaagg
    10681 tccgcctccg tctccgatcc ccgcttccgg tccccgcttc cgggcgctca gggctccgga
    10741 ggcgaggtcg tacattgcgt ggagggtctc cgcatatttc tcccaggcca gtccgtccac
    10801 ggacagcccc ttctcccggg tacagccgtc cacggcgccg gcatcgacca gggcggtcag
    10861 gagggcccgc tcctggtccg gggtacggag gagcaggacg ccggacaaca gggacagggc
    10921 cgcggccagg gcgtagcctc cccagttcga gatcccgcag gccagggtgt gatccgccgg
    10981 ggaggcgcag aagatgaggt ccccccggga tacctcggct ttgagggttt cccggaggcc
    11041 gcccatgccc agctcgttgc cgccgtcccc cacggccagg gttacccagc cgcgcccggg
    11101 atcggggctg aacaggcgat ccgcgg
//
LOCUS       DAOTEI010000114        11097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4157_length_11097_cov_4.354012, whole genome shotgun sequence.
ACCESSION   DAOTEI010000114 DAOTEI010000000
VERSION     DAOTEI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_11097_cov_4.354012"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1093)
                     /gene="hflK"
                     /locus_tag="P5313_11225"
     CDS             complement(119..1093)
                     /gene="hflK"
                     /locus_tag="P5313_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRY80977.1"
                     /translation="MPPKFREPMRLNLKPRTVIVIVIVLAAGIFLGTSFFVVDTTERG
                     VLTTFGQYTKTAEPGLHWKIPFGIQRHYNVKTEVVQVEEFGYRTERLGVQAVYSDKEY
                     PDEATMLTGDLNIVDVEWIIQYRITDPKAWLFNFSASERTRTIRDISQSAMNMLVGDR
                     TILNVLGPERVAIETACLEIMNETLAGYDMGVRIIAVKLQNIVPPKGVQQAFEDVNKA
                     IQDMNRLINEGKEAYNQEIPKARGQADQQIQIARGYAAERVNKARGDVARFASVYNEY
                     RKAPEVTRERLYYEMIEQVFEGETSVELIDKELSNFIPLKNLGTGGNR"
     gene            complement(1171..2139)
                     /locus_tag="P5313_11230"
     CDS             complement(1171..2139)
                     /locus_tag="P5313_11230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY80978.1"
                     /translation="MALRDSFIAGRDDDGKRVDRVVRRLLGDVPLSAVFKALRTGSVR
                     VNGRKPQPADRVREGDAFAITDLRFAAASATTAPGFRDRDSGAAPDLARLIILETEDL
                     LFLNKPRGVLSHGPGGLDESVLAYLSYKAGKSLSFAPAPLHRLDRNTTGLLVASKTLR
                     GARAFSAALQDRTVEKRYIAVLRGRLDRKDHWVDSLERLSKQRRTLRSALAVHRKAVT
                     LVEPIAANGAFTLADIRILTGLTHQIRAQAALRGRPLAGDVKYGGGAEPHGYILHAYR
                     LDFPEEFEADAPRTVIAPVPEDTALRLRERFGPKILLSAPPGIIPP"
     gene            2250..2735
                     /locus_tag="P5313_11235"
     CDS             2250..2735
                     /locus_tag="P5313_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HRY80979.1"
                     /translation="MQGTPIGSGGLQDLLRNPVFLSAAVSLFTAQFIKALIGLSRNKV
                     KSLRDIAETLLWRTGGMPSSHSALVVAITTSIALTEGVRSSVFTLSFFFSMIVIRDAL
                     GVRRAAGMQARAINALQQDLEDRLEIPSRPVKEIHGHTLPQVLVGGLLGFFFSLAFCT
                     L"
     gene            2745..4301
                     /locus_tag="P5313_11240"
     CDS             2745..4301
                     /locus_tag="P5313_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80980.1"
                     /translation="MSDASPAAGPRASRFLLPFAGALLLAAVSYWILPSAPPFPAEWR
                     NWNLILVRTGPSTEQALRILEDAGIGPVWSESSQTVHLSDFVSTRRVSLAQAQKRLVP
                     GDPRNTPWLEGLTGYFRIQDGELSWSILYIPAAAGSRASRALSRALSPEAWNPPERTR
                     RGGPGLSWIPGIVLCGYWAFTSRRRKWLAPAAALPWLPLWISGRLAAAFGGVWGYLVL
                     FSFMEEVGGEPGSIRAAMGIRRSLRFLPQTLILGLPGILDPSYLPGLCFSLGSSVCLI
                     LGLEYLEIWKACRRVHGVFMGIPLDTRRLREDQDRSLSRRSRAAIAACLAVLGLQGIL
                     FAFPGISRGRVSPRSPLPLPGPVIASRYPDARGVERLLRDRTEPGLPSLADAVAHRVH
                     QEALPYSRIGTRAYGSLESATLERFSPEGAQVVRTRETVAEFDNAWVRNALQEESNTG
                     IGAVLADQGGIVNPRVGFPQEGGVSASLALRDVFFYIIFLAPVVLGTARKGIRLPIHA
                     RGITRVASRR"
     gene            4298..5626
                     /locus_tag="P5313_11245"
     CDS             4298..5626
                     /locus_tag="P5313_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HRY80981.1"
                     /translation="MTVIRTFKYGGITIPPKRLPIEYPVMTAFLPTDALIPLRQHSGA
                     PARRVVEVGESIREGQLVGKAAGAGSSNIHSPIPGIVTSIQRLPDLQGGQVEALAVAL
                     EGSFDRLGRKPETFPWKGMSKHQILRTIADKGVVEDDGSGTPLFERLHDPKGTRGACV
                     LVISCLESDPWNSSGLAILKERGEAIREGIAILQRGLSPVRTLIAVDDPESPFLDRPA
                     RTEPGDEGPSVEAVLLEPRYPQDLPALLKEALSGKKAPSEEMIVLTVGTVASVYDAVA
                     LNRPQIERYITVTGDAVKTPGILRARIGTSIGELLEECGGFSSAPECIVLNGPFRGSA
                     VADLDFPILKTTKSVVALTSAETHRHRTTACLRCGRCILACPMRLDPSRLFKCIVRRQ
                     VAEAVSEGLGACTECGSCAYLCPARLPLVQAFSLARKGAFKAGAAGGKTA"
     gene            5623..6726
                     /locus_tag="P5313_11250"
     CDS             5623..6726
                     /locus_tag="P5313_11250"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRY80982.1"
                     /translation="MKSNRQGVLVSAAPFIHVRESTASLMLLIITCLLPSLAWSVFLF
                     GSDVLVIVGVSVASAMAAEAGLSLVRRPGTLMDGTALLTGLLIGFSMPPSVPLYIPIL
                     SSVFAIAVVKWSFGGLGANWMNPAMAGVVFSHVNWPGALNSWKAPRFLTGVDGVSSVT
                     PLGLLRSASENVEGPPMDLLREAGYRISSLDKALTARLNEGILSGLGARLPEGYVDLA
                     LGIRPGSLGEMAGLFLLAGSIILVARRVVRWEIPAACALVFGILVRLFGTGSEPLFSG
                     DVLFAFFTGSFLLVTFFCATDPSTSPMSRPGLLLYGSGVGALIFLFRRYGAGSEGSAY
                     AVIIMNCLVSLLDSALPAARSRRRSVASQGVGN"
     gene            6723..7259
                     /locus_tag="P5313_11255"
     CDS             6723..7259
                     /locus_tag="P5313_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80983.1"
                     /translation="MNNSAKERAAETAAAAALVALLSTAIALGAMIQDRAESARVAGS
                     VERTAARILVPSGTALNVRPLEKRPSPPVRRAYSISTRDGRPAGLGATVSVEGAAWNA
                     ELLVLMDERGRIVSTVPREGSSGIRAEELAVFLRQREKKSKGGLGAAADPGELEFSVN
                     RALDAASRLPNSDLGGGK"
     gene            7256..7870
                     /locus_tag="P5313_11260"
     CDS             7256..7870
                     /locus_tag="P5313_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRY80984.1"
                     /translation="MTLIADKTGPSNPVLLIVTGLCPMLIPSITLQRALILGIGVWVH
                     AVILAALVPILSRVSGDSIRFNLSLVVSGMVAALYGLGIRAVFPAEAFGLSPFLALIA
                     LNCFGLSVLRGSLRQSGLDRLPEYARSAAWLLLTWLGFAALRELTGSGILTLYATETS
                     RAVLDLRSLIIYPIRLAILPSGAFLLLGYGIALYRLRAKRRKEG"
     gene            7872..8459
                     /locus_tag="P5313_11265"
     CDS             7872..8459
                     /locus_tag="P5313_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRY80985.1"
                     /translation="MSYIGIVFTFVFGGNVLFRYGAGTCSSSTETRAGGGGWPAFLTL
                     GSVSLLSAALHSTIMRYVLYPLGLEALEPLSYVLVVVTLLYSLASVLAAGSGPFAEAG
                     RIAKEQVHSCVVYAASLSAARGGFTMAEAAAAGFAAAAGWWCAVVLLDRILRRLEMED
                     VPPALKGAPLRFLSAGLMAMAVSGIDQILVSRIAG"
     gene            8462..9301
                     /locus_tag="P5313_11270"
     CDS             8462..9301
                     /locus_tag="P5313_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY80986.1"
                     /translation="MRTEMLAALFGVSFVFFAAGSYILSSLKRARVDSLVVNTLASLP
                     AWDCGLCGQDDCKSFARVLAAGPVDSRCIPGGAPVERRLTAVLGRAPYRKKNGKARAV
                     VACAGDERAVRPTFEYAGYRDCAAAAGLYGGPRACGSGCLGYGNCVPACPNDAIAVRN
                     GLAEIRPDLCDGCGSCVAACPTGVIRMLPRRDAWYVACSSTAPGKVKEDSCAASCTAC
                     GACERRSAGSEFSLKNNLAVASYNATGNWAAIAEDCPTRVIRTLGAEKKDAGSFGTRG
                     GGL"
     gene            9356..>11097
                     /locus_tag="P5313_11275"
     CDS             9356..>11097
                     /locus_tag="P5313_11275"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HRY80987.1"
                     /translation="MRKTHLVLGSYNHLPEGTEESVFEETYQTCYRPFLSVLYRFPDI
                     FASIHYSGSLLRWLESRHPEFLMLLEEMVLRKQIELLGGGFFAPLLPLLSNADRLGQI
                     EYLTTFIRKNFGRRPRGCWIPEYAWEPSLASSLQTSGMDFTFLPVSHFRAAGFGENEY
                     FAPVLTEDQGRALAVYPVFDCQLSFGTSLGLEETLEAVRTRHGLDHPLIAVFLAGEAV
                     KGLWSRSVYESPDVYLEKTFTGIRKESLSMDTVTPARYLRSMRHFKRTYFPSGATERY
                     LASGLCARLKRERSAAQKNLNGEGERFLSLGGIRLPLLRYEEGVSLYAKMQYVHLLTG
                     QLRGDKSRKKTAQEELWRGQSGDAYWYKPGGGIYRLSLRRAVYEALIEAEKTTRQKGA
                     FSSGIIQADIDFDGVKEILFQGTDINAYLHARGGVLFELDSLKTSRNLSNTFGRYEEL
                     EYSGPLGPRRRCFLDRFQAGPSGPEESVRCGTVDLGNFADSYFSVRELKRSNQEIGLI
                     RDGLVSLGERKIPVTVEKVFQFRRTGVTVRYTITNRGSDDLSCVYACDLNLSPSSRPQ
                     KLVLAAGLSDRTVEL"
BASE COUNT         1761 a         3595 c         3590 g         2151 t
ORIGIN      
        1 atctggccga agcgcacgac tacggcctgc tctccctcgt tgaccacgta gaaaggaccg
       61 gccgccacca ggatgatggc gaggaggacc aggaccacaa gcagggtgat gaactttttc
      121 atcggtttcc tcccgttccc agattcttga gcgggatgaa gttggagagc tccttgtcga
      181 tcagctccac cgaggtttcc ccttcgaata cctgctcgat catctcgtag tagagccgtt
      241 cccgggtgac ctcaggagct ttccggtact cgttgtagac cgaggcgaac cgcgccacgt
      301 ctcctcgcgc cttgttgacc cgctccgccg cgtagccccg ggcgatctgg atctgctggt
      361 ccgcctgtcc ccgggccttc gggatctcct ggttgtaggc ttccttgcct tcgttgatca
      421 gccggttcat gtcctgtatg gctttgttga catcctcgaa agcctgctgg acccccttgg
      481 ggggcacgat gttctggagc ttgaccgcga tgatccggac tcccatgtcg taccccgcca
      541 gggtctcgtt catgatctcc aggcacgcgg tctctatggc cacgcgttcg ggaccgagga
      601 cgttcaggat ggtccggtcc ccgaccagca tgttcatggc ggactgggag atgtcccgga
      661 tggtgcgggt ccgctcggag gcggagaaat tgaagagcca ggccttggga tccgtgatcc
      721 ggtactggat gatccattcc acgtccacga tgttcaggtc tccggtcagc atcgtggcct
      781 catccggata ctctttgtcc gagtataccg cctgtacgcc caggcgttcc gtccggtaac
      841 cgaactcctc gacctggacg acttcggtct tgacgttgta gtgcctctgg atcccgaagg
      901 gtatcttcca gtgcagcccc ggttcggcgg tcttcgtgta ctgcccgaag gtcgtcaaca
      961 cgccgcgttc cgtggtatcc accacgaaga aactggtacc caggaagatc cccgccgcga
     1021 ggacgatgac gatcacgatg acggtgcgcg gtttcaggtt caggcgcatg ggttcgcgaa
     1081 atttcggggg catcctcacc tccttttcgg gaccgctcgc tcgggacgcc atccctcaag
     1141 gatcaagggt actgactatg cgggcggggt tcaaggagga atgatcccgg gcggggcgga
     1201 tagcaggatt ttcggcccga atcgttcccg gagccgcaga gcggtgtcct cgggcaccgg
     1261 ggctatgacg gtacgcgggg cgtcggcctc gaattcctcc ggaaaatcga gtcggtacgc
     1321 gtgcaggatg tatccgtgcg gctccgcacc tccgccatac ttgacgtccc ccgccagggg
     1381 ccggccccgc agggcggcct gggcccggat ctggtgggtc aagcccgtga gaatccggat
     1441 gtccgccagg gtaaacgccc cgttcgcggc tatgggttcc accagggtga ccgcttttcg
     1501 gtgcacggcc agggcggatc gcagggtccg cctctgcttg gaaaggcgct ccaggctgtc
     1561 gacccaatgg tccttgcggt ccaggcgacc ccgcaatacg gcgatgtatc gtttttctac
     1621 ggtccggtcc tgaagggccg cgctgaaagc ccgcgcaccc cgaagtgtct tggaagctac
     1681 caggaggccc gtcgtgttcc tgtccaggcg atggaggggg gccggggcga aggaaaggga
     1741 tttcccggcc ttgtaggata gataggccag gaccgactcg tccaagcccc cggggccgtg
     1801 ggagagcact ccccggggtt tgttcaggaa gaggagatcc tcggtttcca gaatgatgag
     1861 acgggcgagg tccggagcgg caccggaatc gcggtcccgg aagcccgggg cggtcgtggc
     1921 ggaggccgcg gcgaagcgca ggtcggtgat cgcgaaagca tccccttccc gcacgcggtc
     1981 ggcgggttgg ggctttcggc cgtttacgcg aaccgatcct gttcgaaggg ctttgaatac
     2041 cgcggaaagg ggaacgtcgc ccaacaaacg ccggacgacg cgatccacac gcttgccgtc
     2101 gtcgtctcgt ccggcgatga agctatcccg aagggccatg cctcatccta cgcgttgtat
     2161 caaaatgagt caaggaaaag tatccgtatc ccggtcctca gggggtccga tgccttgaca
     2221 atccttcccc gctgggggag agtaccctaa tgcaggggac gccgatcggt tcgggggggc
     2281 tgcaggatct cctgcgcaat cccgtctttc tttccgcggc cgtaagtctc tttacggccc
     2341 agttcatcaa ggcgctcatc gggctgtccc ggaacaaggt aaagtccctc cgggacatcg
     2401 cggagaccct cctgtggcgc accggaggga tgccctccag ccacagcgcc ctcgtggtcg
     2461 ccataacgac ctccatcgcc ctcacggaag gggtccggtc cagcgtcttc accctgtctt
     2521 tcttcttttc catgatcgtg atccgggacg ccctgggggt ccgccgggcg gcgggcatgc
     2581 aggcccgggc gatcaacgcc ctgcagcagg acctggaaga ccggctcgag attccgagcc
     2641 ggcctgtaaa ggaaattcac ggtcataccc tgccccaggt tctggtgggc ggccttctgg
     2701 gcttcttttt ttccctggcg ttctgcacgc tatagcccgc cgtgatgtcc gacgcttccc
     2761 cggcagccgg ccccagggct tctagattcc tcctaccctt cgcgggagcc ttgctcctgg
     2821 ccgccgtgtc ctattggatt cttccttcgg ccccgccgtt cccggccgag tggaggaact
     2881 ggaacctgat cctggtccgg accggtccgt ccacggaaca agcccttcgg atcctcgagg
     2941 acgcggggat aggccccgtc tggtccgaat cctcccagac cgtccatctt tcggacttcg
     3001 tgagcacaag aagggtaagc ctggcgcagg cccaaaaaag gctggtcccc ggggatcctc
     3061 ggaacacccc ctggctggag ggccttacag gctatttccg cattcaagac ggcgagctgt
     3121 cctggagcat cctgtatatc cccgccgcgg cgggttcccg agcgtcccgg gccctgtccc
     3181 gggccctctc cccggaagcc tggaacccgc ccgaacgaac ccggcgcggc ggtccgggtc
     3241 tgtcctggat tcccggaatc gtcctgtgcg gatactgggc gttcacatcg cgccgccgga
     3301 aatggctggc cccggccgcc gcccttccct ggcttccgct ctggatttcc gggcgtctcg
     3361 ccgcggcgtt cggcggcgtc tggggatacc ttgtcctgtt ctcttttatg gaagaggtcg
     3421 gaggggagcc gggatcgatc cgggccgcta tggggatccg gcggagcctg cggttcctgc
     3481 ctcaaaccct tatcctgggg ctcccgggta ttctcgaccc gtcctatctt ccgggactat
     3541 gtttttccct gggctcatcg gtctgcctga tcctagggct tgagtatttg gagatatgga
     3601 aggcttgtcg acgggtccat ggggtgttca tgggcattcc cctggatacc cgacggcttc
     3661 gagaggatca ggacaggagc ttgagccgcc ggtcccgggc cgcaatagcc gcgtgtctgg
     3721 ctgtcctggg cctccagggg atccttttcg ccttcccggg tatttctcgg ggaagagttt
     3781 ccccccggtc accccttccc ctgcccggac ccgtgatcgc gtcgaggtac ccggacgccc
     3841 gaggcgtgga gcggctcctg cgggatcgga ccgaacccgg cttgccctcc ctggcggacg
     3901 cggtggccca ccgggtacac caggaagccc tcccgtattc caggatcggt acccgggcgt
     3961 acggcagcct ggaatcggcg accctggagc gattcagccc ggaaggggcc caggttgtcc
     4021 ggacccggga aacggtagcg gagttcgaca acgcctgggt tcggaacgcc cttcaggagg
     4081 agtccaatac cgggatcggg gccgtgctgg ccgatcaagg gggcatcgtg aatccccggg
     4141 tgggattccc tcaggaaggc ggggtttccg caagccttgc gttaagggat gttttctttt
     4201 atataatctt cctcgcgccc gtcgtgctgg gaacggcacg gaagggcatc cggcttccga
     4261 tccatgcccg gggaatcacc cgggtcgcat caaggagatg accgtcattc gcaccttcaa
     4321 atacggagga atcacgattc ccccgaaacg gctgccgatc gaataccccg tcatgacggc
     4381 ctttctgccg accgacgcat tgatccctct acggcagcat tccggcgctc ccgcccggag
     4441 ggtcgtggag gtcggggagt cgatccggga aggccagctc gtgggaaagg ccgcgggggc
     4501 gggatcctcc aacattcact ccccgattcc gggcatcgtt acgagcatcc aaaggctgcc
     4561 ggatctccag ggaggccagg tggaggcgct tgccgtcgcc ctggagggat ccttcgaccg
     4621 gctgggcagg aagccggaaa cctttccctg gaaggggatg tccaagcatc aaatcctcag
     4681 gacaatcgcg gacaaggggg tcgtggagga tgacggttcg ggtacccccc tgttcgaacg
     4741 cctccacgat cccaagggga cgcggggggc ctgcgtcctt gtcatcagct gcttggagag
     4801 cgatccctgg aattcatccg gcctggccat actcaaggaa cggggggagg cgatccggga
     4861 gggtatcgcc atcctgcaga ggggcctgtc gcccgtgcgc accctgatcg ccgtggacga
     4921 cccggaatct cccttcctgg accggcccgc acggaccgaa cccggggatg aggggccttc
     4981 cgtggaggcc gtgctcctgg agccccggta cccccaggat ctccccgccc tcctgaagga
     5041 agccctgtcc ggcaagaaag cgccttccga ggagatgatc gtacttaccg tcggcaccgt
     5101 cgcctccgtg tacgacgcgg tggccctgaa caggccccag atcgagaggt acatcaccgt
     5161 caccggagac gcggttaaga ctcccggcat cctgcgggcc cgaatcggca cctccatcgg
     5221 ggaattgctg gaggagtgcg gcggattcag ctcggccccg gagtgcatcg tcttgaacgg
     5281 tcccttccgg ggcagcgccg tggcggacct ggatttcccc atcctcaaga ccacgaaatc
     5341 cgtcgtggcc ctgacctccg cggaaaccca ccggcatcgc accacggcgt gtctccgttg
     5401 cggccgctgc atcctggcct gtcccatgcg gctggacccg tcccgcctct tcaagtgcat
     5461 cgtgcggaga caggtcgccg aggccgtgtc cgagggtttg ggcgcctgta cggagtgcgg
     5521 aagctgcgcc tatctctgcc ctgcccggct tcccctggtt caggcctttt ccctggcccg
     5581 gaagggagcc ttcaaggcgg gagcggcggg aggaaaaacg gcatgaagtc gaaccgtcag
     5641 ggcgtcctgg tttccgcggc acccttcatc cacgtgcggg aatccacagc gagcctcatg
     5701 ctgctcatca tcacctgcct cttgccgtcc ctcgcctggt ccgtcttcct gttcgggtcg
     5761 gacgtcctgg tgatcgtggg ggtctccgtg gcttcagcca tggctgcgga agccggtctg
     5821 agcctggtcc ggaggccggg aaccctcatg gacggtacgg ccctgctgac gggcctcttg
     5881 atcgggttct ccatgccccc ctcggttccc ctgtacatcc cgatcctctc ctcggtcttc
     5941 gcgatcgcgg tggtaaagtg gtccttcgga ggtctcgggg ccaactggat gaaccccgcc
     6001 atggccgggg tcgttttctc ccacgtcaac tggcccgggg ccctgaacag ttggaaagcc
     6061 cccaggttcc tcacgggggt ggacggggtt agctccgtga ccccactggg attgctccgg
     6121 agcgcatcgg aaaacgtcga aggtccgccc atggacttgc ttagagaggc ggggtacagg
     6181 atctcgagcc tggacaaggc cctaaccgcc cgtctgaacg aggggatcct ctcgggattg
     6241 ggggcgcgtc tgccggaagg ctatgtggac cttgccctgg ggatacgccc gggatctctg
     6301 ggagaaatgg cggggctctt cctgctggcg ggatccatca tcctggtagc acgccgcgtg
     6361 gtccgatggg agatccccgc cgcctgcgcc ttggtcttcg gcatcctggt ccggctcttc
     6421 ggtacgggtt ccgaacccct cttctccggg gacgtgctgt tcgccttctt cacgggcagc
     6481 tttctcctgg tcacgttctt ctgtgccacg gatccctcga cctcgcccat gtcccgtccg
     6541 ggcctgctcc tgtacggaag cggagtcggc gccttgatct tcctgttccg gcgatacggc
     6601 gcgggatcgg agggatccgc ctatgcggtc atcatcatga actgcctggt atccctgctg
     6661 gattcggcct tgcccgcagc gagaagccgg cgccgctcgg tcgcatccca gggggtgggg
     6721 aattgaacaa ctccgcgaag gaacgggctg cggaaaccgc cgcggcggcc gccctggtcg
     6781 ccctcctgtc caccgcgata gccttgggcg ccatgatcca ggaccgcgca gaatccgcca
     6841 gggtcgccgg ttccgtggaa cggactgcgg cgcgcatcct ggttccatcc gggaccgcgc
     6901 tgaacgtccg gcccctggag aaacgacctt ctccccccgt gcgcagggcc tattccatat
     6961 ccacccggga cggaaggcct gcgggtctcg gcgcgacagt ctctgtcgag ggagccgcct
     7021 ggaacgcgga actcctggtc ctgatggatg agcgggggcg gatcgtctcg acggttccga
     7081 gagaaggttc gtccggaata cgggccgagg aactggcggt cttcctccgt cagcgagaga
     7141 agaagtccaa gggcggcctg ggtgcggccg cggatcccgg ggaactggag ttttccgtga
     7201 accgtgccct ggatgccgcg tcccgcttgc cgaactcgga tttggggggt gggaaatgac
     7261 cctgattgct gataaaaccg gcccctcgaa cccggtgctc ctgatcgtga cggggctctg
     7321 cccgatgttg atcccttcga tcacgctaca gagggccctt atcctgggaa tcggcgtctg
     7381 ggttcacgcg gtcatcctgg ccgccctggt gcccatcctt tcaagggtct ccggggactc
     7441 catccggttc aacctgtccc tggtcgtgtc cggtatggtc gcggcgctct atggactggg
     7501 tatacgcgcg gtgttcccgg ccgaggcctt cggattgtcg ccctttctgg ccttgatagc
     7561 cctcaattgc ttcgggctct ccgttttacg gggttccctg cgccagtcgg ggctggaccg
     7621 cttgccggaa tacgcccgct ccgcagcctg gctcctgctg acttggctgg gattcgcggc
     7681 cttgcgggaa ctgacgggtt ccggaatcct gaccctgtac gccacggaaa cgtcccgtgc
     7741 ggtgctggac ttgcggtccc tgatcatcta tcccatccgt ctggccatcc tgccctcggg
     7801 agccttcctg ctcctggggt acggaatcgc cctgtaccga ctgcgcgcca aacggcggaa
     7861 ggaggggtga gatgtcctat atcggaatcg tgttcacctt cgtcttcggc ggcaacgtgc
     7921 tcttccgcta cggcgccggg acatgctcga gctccacgga aacgcgggct ggaggcgggg
     7981 gctggcccgc cttcctcacc ctgggctccg tcagcctcct ttcggcggcc ctgcattcca
     8041 cgatcatgcg gtacgtgttg tatcccctcg ggctggaggc cctcgaacct ctttcctatg
     8101 tacttgtcgt cgtgaccctc ctgtactccc tggcctcggt tctggctgcc ggctccggcc
     8161 ccttcgcaga agcggggcgg atcgcaaagg aacaggtcca cagctgcgtc gtgtacgccg
     8221 cctccctgtc cgcggcacgg gggggcttca cgatggcgga agccgcagcg gccggattcg
     8281 ccgcggccgc ggggtggtgg tgcgccgtgg tcctgctgga tcgaatattg cgtcggctgg
     8341 agatggagga tgtgccgccc gcgctgaagg gcgcgcccct ccggttcctg tccgcgggtc
     8401 tcatggccat ggccgtgtcc ggaatagacc aaatcctggt ttccaggatc gcggggtagt
     8461 cgtgaggaca gaaatgcttg cggccctgtt cggggtctcc ttcgtgttct tcgcggcggg
     8521 gtcctacatc ctgtcctccc tcaagcgggc gcgcgtggat tccctagtcg tcaacaccct
     8581 cgcgagcctg ccggcctggg actgcggcct ctgcggccag gacgactgca agtccttcgc
     8641 acgggtcctg gcggcggggc ccgtggattc ccggtgcatc cccgggggcg ccccggtgga
     8701 acggcgcctg accgccgtcc tgggaagggc cccctaccga aagaagaacg gaaaggcccg
     8761 agcggtcgtg gcctgcgcgg gggatgaacg cgcggtacgg ccgaccttcg agtacgccgg
     8821 gtaccgggac tgcgcggccg cggcggggct gtacgggggc ccgcgggcct gcggctccgg
     8881 ctgtctcggc tacggtaatt gtgtccccgc ctgcccgaac gacgcgatcg ccgtgaggaa
     8941 cggactcgcg gagatccgtc cggacctgtg cgacggctgc gggtcctgcg tcgcggcgtg
     9001 tccgaccggt gtcatccgca tgcttccgcg cagggatgcc tggtacgtgg cctgctcgtc
     9061 cacggccccc ggaaaggtga aggaggattc ctgcgccgcc tcctgcacgg cctgcggggc
     9121 ctgcgagcga aggtccgcgg gaagcgagtt ctccctcaag aacaacctgg ccgtggcttc
     9181 ctacaacgcc accggcaact gggcggccat cgcggaggac tgcccgacaa gagtcatccg
     9241 gaccctgggg gcggaaaaaa aggacgccgg atctttcgga accaggggcg gtggattata
     9301 atcccttcca gcatcgggcg cggaccctcg gcgggcgtat aaggatgtcg ttcggatgag
     9361 aaaaacccat ctcgtgttgg gtagctacaa ccatctcccg gagggcaccg aggaatccgt
     9421 cttcgaggaa acctaccaga cctgctaccg ccccttcctg tccgtcctct atcgttttcc
     9481 cgacatcttc gcctccatcc actattccgg tagcctcctg cgctggctgg agagtcggca
     9541 tcccgaattc ctcatgctcc tcgaggaaat ggtgctccgg aagcagatcg agctgctggg
     9601 gggcggcttt ttcgcacccc tgcttcccct tctgtcgaac gcggaccgac tggggcagat
     9661 cgagtacctg accacgttca tccggaagaa cttcggcagg cggccccggg gatgctggat
     9721 ccccgagtac gcctgggagc cttccctggc atcctcgctc cagacctcgg ggatggattt
     9781 caccttcctg ccggtttccc atttccgggc ggccggcttc ggggagaacg aatacttcgc
     9841 ccccgtgttg accgaggatc agggaagggc cctggcggtc taccccgtgt tcgattgcca
     9901 gctgagcttc gggacgtccc tgggattgga ggagaccctg gaagccgtcc ggacgcgcca
     9961 cggactggat catcccctga tcgcggtctt cctggcggga gaagccgtca aggggctttg
    10021 gtcgcgaagc gtctacgagt cgcccgacgt gtacctggaa aagaccttca cggggatacg
    10081 gaaggaatcc ctatccatgg acaccgtgac tccggcccgg tacctgcgga gcatgcgcca
    10141 cttcaagcgc acctatttcc ccagcggggc caccgagagg tacctggcct ccgggctctg
    10201 cgcgcggctg aagcgggaac ggtcggcggc ccagaagaac ctgaacgggg agggcgagcg
    10261 attcctctcc ctgggcggca tacggcttcc cctgttgagg tacgaggagg gagtctccct
    10321 ctacgcgaag atgcagtacg tccacctcct gaccggacag ctgcgcggcg acaagtcccg
    10381 taagaagacc gcccaggagg aactctggcg gggccaatcc ggggacgcct actggtacaa
    10441 gcccggggga ggaatctacc ggctttccct gcggagggcc gtgtacgaag ccctgatcga
    10501 ggcggaaaag acgacccggc agaagggggc gttcagttcg ggcatcatcc aggcggatat
    10561 cgacttcgac ggagtcaagg agatcctttt ccaggggacg gatataaacg cctatctcca
    10621 cgcccggggc ggggtgctgt tcgaactgga ttccctgaag acctcccgca acctatccaa
    10681 caccttcggg cggtacgagg agctggagta ttcaggacct ctcggccccc gtcgcagatg
    10741 tttcctggac aggttccagg ccgggccgtc cggaccggag gagtccgtcc ggtgcgggac
    10801 cgtggacctg ggcaacttcg cggattccta cttcagcgtc cgggaactga agcgctccaa
    10861 ccaggaaatc ggtctcatcc gggacggcct ggtatccctg ggggagcgca agatcccggt
    10921 gaccgtggag aaagtattcc aattccggag gacgggagtc acggtgcggt acaccatcac
    10981 gaaccggggc tccgacgacc tttcctgcgt ctatgcctgc gacctgaacc tgagcccctc
    11041 ctcccgaccc cagaaactgg tgctggccgc ggggctttcg gacaggaccg tggagct
//
LOCUS       DAOTEI010000115        11086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4163_length_11086_cov_5.582993, whole genome shotgun sequence.
ACCESSION   DAOTEI010000115 DAOTEI010000000
VERSION     DAOTEI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4163_length_11086_cov_5.582993"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..636
                     /locus_tag="P5313_11280"
     CDS             163..636
                     /locus_tag="P5313_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY80988.1"
                     /translation="MAAVQTEFSADLVRFIEEWKRKPGSLIMMLHRIQEEFGYISRES
                     AETLAKMIDMPMAKIYGVVTFYHFFKTTKPGKNRLAVCMGTACYLKGGQTLIEECESI
                     LNINAGEVTDDGQFSIDSVRCVGCCGLAPVLMVGNDVYGKLTKDQLPEIIAKYRN"
     gene            642..1205
                     /locus_tag="P5313_11285"
     CDS             642..1205
                     /locus_tag="P5313_11285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY80989.1"
                     /translation="MHASVCDYVLDVVQNSVEAQARNVELDLFEEGGRISVRVADDGR
                     GMTEEERSRALDPFRSSGGKHPGRRVGLGLPFLEQAVRAAEGEFSLESRKNQGTTLRF
                     SFPSGHLDTPPLGDVPGLFRSALAFDGAYELRIRRRREARAGAPGTEYEAARSELREA
                     AGDLSCASALILVGRYLESLEYPGTDG"
     gene            1239..1634
                     /locus_tag="P5313_11290"
     CDS             1239..1634
                     /locus_tag="P5313_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRY80990.1"
                     /translation="MAKMTLEDLRKLRDERKKDLGRRDVEGKSVQIIVGMGTCGIAAG
                     AKKTFDAILAAVEEFHLDKDVVIRQTGCMGLCYVEPTVEVAMPGMPATIYGKVDAAVA
                     REIVQKHVVEKSLVENHIFDRPAADIIKR"
     gene            1651..3438
                     /locus_tag="P5313_11295"
     CDS             1651..3438
                     /locus_tag="P5313_11295"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRY80991.1"
                     /translation="MAYKNFILVCGGTGCESSKADEIYKNLLAEAEAQGVKGEVQVVK
                     TGCFGFCEKGPIVKVLPSESFYVEVKPSDAKEIISEQIVKGREVKRLLYKKEQQKDIV
                     KLEDIEFYQKQFRIVLRNCGVINPENIEEYIARDGYAALEKVLFEMKPDDVIAELKTA
                     GLRGRGGAGFPTWMKWDLTRKAAGDIKYVICNADEGDPGAYMDRSTIEGDPHSVLEAM
                     TIAGYTIGSDKGFVYIRAEYPLAIERLKIAIAQSKAMGLLGDDILGSGFNFDIEIRLG
                     AGAFVCGEETALMQSLEGKRGMPVPKPPFPAISGLWKKPTLINNVETYANVPVILTKG
                     GSWFAKIGTEKSPGTKVFALTGKINNSGLVEVPMGTTLREIVFDIGGGIKDGKKFKGV
                     QTGGPSGGIITEKDLDSPITYESLTALGSMMGSGGMIVMDEDDCVVDVSRFYMEFCVD
                     ESCGKCAPCRIGTNQMHSILTKISKSQGEMSDLDKLERIGKAMTKASLCLLGGSAANP
                     TLSTLKHFRDEYLEHIQDRKCRAGKCKDLVVYSIDPEKCIGCGLCARRCPVNCITGEK
                     KQAHVIDTSKCIKCGECFKVCKFNAVLKK"
     gene            3461..5212
                     /locus_tag="P5313_11300"
     CDS             3461..5212
                     /locus_tag="P5313_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRY80992.1"
                     /translation="MVNAKINGIPVHVAKGTTILEAARKVNVKIPKLCHNNDLEPWAA
                     CGICVVKNGKSPKMLRACCTPLEDGMDIITNDAELVATRRTVIELILSTHPDDCLQCP
                     RNGDCELQTLAAEFGIREQSFPKRLRGLATDESTGTIILNPEKCIRCGRCVKVCQDMQ
                     GVWAIEFLGRGEKTRIAPAADVALAEGPCIKCGQCSAHCPVGAIYEMDQTKVVWDALM
                     KEGPDAKTCVVQIAPAVRVALGEAFGLEPGTVITKKIYAALRRLGFDAIFDTSFSADL
                     TIMEEGTEFVKRFTTDDGPLPLITSCCPAWVDYMEKYYPDMIPNFSTAKSPQQMMGAM
                     IKTYWAEKASTDPSKIFSVSIMPCTAKKYENSRNDDMYSSGQKDVDVTLTTRELARMI
                     KQAGIDILNLPDEEPDSPMGPYTGAGVVFGATGGVMEAALRSAYYLITDEDLKDVNFT
                     GVRGISGVKEARVHIKGTDVRVAVAHQMGNIAMVLEEVRKAKAEGKEVPWHFIEVMAC
                     RGGCIGGGGQPYGCTDEVRQKRMAGIYADDEKSEYRCSHRNPYIGQVYKEFLEKPGSH
                     KAHKLLHTHYTRKPVYK"
     gene            5332..6573
                     /locus_tag="P5313_11305"
     CDS             5332..6573
                     /locus_tag="P5313_11305"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRY80993.1"
                     /translation="MEDIDFRASARTVLEETDRLVRRYGPRLAGSEACRSVARELAAR
                     LEGFCDRVRLEEFRAHPASFYAYTKILPLVYVLGLVSLLLNRGFYLFPALGIAGGIAL
                     MVSQFAVYGRLGDGLFPARTCVNVEGVVEPEGKAEYELLLSGHHDSAPVARIYSGPFQ
                     KFYAAAIFAPYPFFLLELGLLAARAAGWIRAVPAGWSAALLAGLPFAAAYFLMIDTRR
                     GSPGAGDNLVSSLLCVEIARELAARKGDLLRRTRIRVLSFDAEEAGLRGSAAYMRSRA
                     GEIRDRAAWMLNFDSLYDAAHLHVLDRDVNGFLRLSREVAQDAVECLRADGYKARVFP
                     MVFGGGGTDAAEAARAGIEAATVIAMPTAVVREGLVYHTPRDTPDAVGVDAVEACLRL
                     TARYAGRIESGTSRRRKGGVR"
     gene            complement(6746..7402)
                     /locus_tag="P5313_11310"
     CDS             complement(6746..7402)
                     /locus_tag="P5313_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HRY80994.1"
                     /translation="MSSKTVASFFGWSNTGKTTFIEGAICALSARGLPCGALKVTKHP
                     GSFLIPGKDSTRFFEAGAETALVGGPETVLTLRSPGTLDRAFLDRLFPDARVILVEGG
                     EVDGALRVLAAGAAASEADLKVPLERVDVLITGDPGLAARAVGAGVRSLAPDDIDEFI
                     KLLEDRMERDVTVTCGGKNVPMNPFVKGLVRDVSLAVINSLKKVEEGEEIVIKIAAPK
                     "
     gene            7458..7958
                     /locus_tag="P5313_11315"
     CDS             7458..7958
                     /locus_tag="P5313_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY80995.1"
                     /translation="MRTRRPILRAAPSLAGLSVPAVLALILAACASGGAVKSGLSAVY
                     APGLTVTLVSWKDGQAEYAGNPASGRWLTVDARIDYDGNAPSAELPAFALRVRWEAPD
                     GSRSESAPLWVYDPTPGAGGAAKRDSLRVRISNRFILAYDLPVDAIPLSFAVGGTDLP
                     LTAPSP"
     gene            complement(7964..9160)
                     /locus_tag="P5313_11320"
     CDS             complement(7964..9160)
                     /locus_tag="P5313_11320"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HRY80996.1"
                     /translation="MPDSRSPENPLASRVEALLKRRHELVGLPRGRPGEPGSSEGLTH
                     SEYARMSDALFRLQRGLTGNRSLIGARYMDDPDLLAAYLFFYWPVSYAQVAGVLAQSG
                     LRPARVLDMGSGPGPATAALLDAGAVSAVLVDSSSSALDLARRVLAQGRASSDRLRYL
                     AADLEGPAPYGGAADAGPYDCVVFGHSLNELWIGAHDRPERVLALAAAASGCLSTEGF
                     LLVVEPALLATSRDALALRDGLAAAGWSVFAPCPGRSRLGCPALAAGPAHTCHDELRW
                     ETPETVRVLAKRTGLDKDSLKATWFLARPPSAPAALPRSAYPEDSFRVVSDPLLNKAG
                     RMRYLLCGAAGRFPFSAPAEDPRSRTSGFSYLRRGDVIRVENPEIRESGWGLGPATRI
                     EVLPGA"
     gene            complement(9202..9888)
                     /locus_tag="P5313_11325"
     CDS             complement(9202..9888)
                     /locus_tag="P5313_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRY80997.1"
                     /translation="MLDTILPEGLRFGVLFDRLTSVHSIYIRAEEASASFRARNGLSC
                     PPGCGRCCEGFIPDILPLEASYLAAWLVRNRPERALRMADSGTLPSNLRGCPLYDPDR
                     PDAHCTVYPGRPLICRLFAFSGVRTRTGSPSYSLCREMPGLPPPRSGTRSWEGPALED
                     LFGTPPLMSDFGAELEGIVPGDGSRREALPEALPAAICQILFLAGLGGKDDPSTDDND
                     PVLPPLPRAS"
     gene            complement(9936..>11086)
                     /gene="uvrC"
                     /locus_tag="P5313_11330"
     CDS             complement(9936..>11086)
                     /gene="uvrC"
                     /locus_tag="P5313_11330"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrC both incises the 5'
                     and 3' sides of the lesion. The N-terminal half is
                     responsible for the 3' incision and the C-terminal half is
                     responsible for the 5' incision; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HRY80998.1"
                     /translation="FEEAARARDTLQAIEAFRGESRVVDFDPESRDYVAAALDGSLAT
                     FTVFAMRGGRLAGRDLFRSRAFGTPEEAVLDFLTTYYSAERPPPPRIYLRDPRGMELA
                     REYLRRELGAETDLAAPEDRRHEAALAMAEHNAKEDLAKRRRETGDVEALEELRRTLD
                     LEVLPARIEGFDIAHLSGKYTVASLVTFSNGVPDKKNYRHFRIRSLEGKIDDFAALRE
                     AVGRRYTGLLNEGEDLPDLILVDGGIGQVNAVKEILDALEADLPLAGLAKENEEIWLP
                     GAEGPIVLSKDSPALRVLVAVRDETHRFATGLNQRLRGKTLRLTGLEAAEGVGPVRAA
                     RILTAFGSLRAVAEAEPAYIAEKARVPKKTAEAAREAARSELSAGRID"
BASE COUNT         1921 a         3615 c         3842 g         1708 t
ORIGIN      
        1 ttccataggt cggcggcggg tccgaattga taaaatctac cgattaaagc aggaattttc
       61 cggaccgccc ccgggcgacc ctattgtcat ctttttggtt ctttcatata ctacggaaag
      121 ttaattatcg atattttctt atatatattc ttggaggaat tcatggctgc agtccagacg
      181 gaattttccg cggatctcgt gcggttcatc gaggaatgga agcggaagcc cggtagcctc
      241 atcatgatgc ttcaccgcat ccaggaggag ttcgggtaca tttcccggga gtccgcggag
      301 accctggcga agatgatcga catgcccatg gccaagatct acggggtcgt caccttctac
      361 cacttcttca agaccaccaa gcccggcaag aaccgcctgg cggtctgcat gggcaccgcc
      421 tgctacctca agggcggaca gaccctgatc gaggaatgcg agagcatcct gaacatcaat
      481 gccggcgagg tgacggacga cgggcagttt tccatcgatt ccgtccgctg cgtgggttgc
      541 tgcggactgg ctccggtgct gatggtcggg aacgacgtgt acggcaagct gaccaaggac
      601 cagctcccgg agatcatcgc caagtaccgg aactgacgcg ggtgcacgcc agcgtctgcg
      661 actacgtcct ggacgtggtc cagaactccg tcgaggcgca agcccggaac gtggagctgg
      721 acctgttcga ggagggcggc cggatctcgg tccgggtggc ggacgacgga cgcggcatga
      781 cggaggaaga gcggtccagg gccctggatc ccttccggtc cagcgggggc aagcaccccg
      841 ggcggagggt gggcctgggg ctccccttcc tggaacaggc cgtccgggcg gcggaggggg
      901 agttttccct ggagtcccgg aagaaccagg gcacgaccct ccggttctct tttccctccg
      961 ggcacctgga cactccgccc ctgggggacg tgccgggcct gttccgttcc gccctggcct
     1021 tcgacggggc gtacgaactg cggatccgaa ggcggcggga agcccgggcc ggagctccgg
     1081 ggacggagta cgaggcggct cggtcggagc tccgggaggc ggcgggggac ctgagctgcg
     1141 cctcggcgct gatccttgtg ggccggtacc tggaatccct ggagtatccg gggacggacg
     1201 gatagaagaa gtacgattcg aaccgtaagg agaaggatat ggcaaagatg accctggagg
     1261 atctccggaa actgcgggac gagaggaaga aggacttggg ccgccgtgac gtggagggga
     1321 agagcgtgca gatcatcgtc ggcatgggga cctgcgggat cgccgcgggg gccaagaaga
     1381 ccttcgacgc gatcctcgcg gccgtggagg agttccacct ggacaaggac gtggttatcc
     1441 gccagaccgg gtgcatggga ctgtgctacg tggagcccac cgtggaggtg gccatgcccg
     1501 gcatgcccgc aaccatctac ggcaaggtgg acgccgctgt ggcccgggag atcgtacaga
     1561 agcacgtcgt ggagaagtcg ctggtggaga accacatctt cgaccgcccc gcggcggaca
     1621 tcatcaagcg gtaaggcgga gggccgattc atggcgtaca agaatttcat actggtctgc
     1681 ggcggaacgg gttgcgagtc ctccaaggcg gacgagatct acaagaacct cctggccgag
     1741 gcggaagccc agggcgtcaa gggcgaggtc caggtcgtca agaccggctg tttcggattc
     1801 tgcgagaagg gacccatcgt caaggtcctg ccctcggagt ccttctacgt cgaggtaaag
     1861 ccctcagatg ccaaggagat catctccgag cagatcgtga agggccggga ggtcaagcgc
     1921 ctcctgtaca agaaggaaca gcagaaggac atcgtaaagc tcgaggacat cgagttctac
     1981 cagaagcagt tccggatcgt cctgcggaac tgcggcgtca tcaacccgga gaacatcgag
     2041 gaatacatcg cccgggacgg ctacgccgcc ctggagaagg tccttttcga gatgaagccg
     2101 gacgacgtga tcgcggagct caagaccgcg ggcctccggg gccggggcgg cgccggcttc
     2161 cccacctgga tgaagtggga cctgacccgc aaggccgccg gtgacatcaa gtacgtcatc
     2221 tgcaacgccg acgagggcga cccgggagcc tacatggacc ggtccaccat cgagggggat
     2281 ccccattcgg tgctggaagc catgaccatc gcagggtaca ccatcggctc cgacaagggc
     2341 ttcgtgtaca tccgggcgga atatcccctt gccatcgagc ggctcaagat cgccatcgcc
     2401 cagtccaagg cgatggggct cctgggggac gacatcctcg gctccggctt caacttcgac
     2461 atcgagatcc gcctgggcgc cggcgccttc gtctgcggag aggagacggc cctgatgcag
     2521 tccctggagg gcaagcgggg catgcccgtt ccgaaaccac ccttcccggc gatctcgggt
     2581 ctatggaaga aacccaccct gatcaacaac gtggagacct acgccaacgt cccggtcatc
     2641 ctgaccaagg gaggctcgtg gttcgccaag atcgggaccg agaagagtcc gggaaccaag
     2701 gtcttcgccc tgaccgggaa gatcaacaat tccggcctcg tggaagtccc catgggcacc
     2761 accctccggg agatcgtctt cgacatcggc ggcgggatca aggacggaaa gaagttcaag
     2821 ggcgtgcaga ccggcggccc ctccggcggg atcatcaccg agaaggacct cgactccccc
     2881 atcacctacg agagcctcac ggccctggga tccatgatgg gctccggcgg catgatcgtc
     2941 atggacgagg acgactgcgt ggtggacgtc tcccggttct acatggagtt ctgcgtggac
     3001 gagtcctgcg gaaagtgcgc cccctgccgg atcggaacga accagatgca ctccatcctg
     3061 accaagatat ccaagagcca gggggagatg tcggacctgg acaagctgga acggatcggc
     3121 aaggccatga ccaaggccag cctctgtctc ctgggcggat ccgccgccaa ccccaccctc
     3181 tccaccctca agcatttccg ggacgagtac ctggagcaca tccaggaccg gaagtgccgg
     3241 gccggaaagt gcaaggacct ggtggtctac tccatcgatc cggagaagtg catcggctgc
     3301 ggcctctgcg cccggcgctg cccggtcaac tgcatcaccg gggagaagaa gcaggcccac
     3361 gtgatcgaca cgtccaagtg catcaagtgc ggcgagtgct tcaaggtctg caaattcaac
     3421 gcggtcctca agaagtgatc cggcaaggag acgaagcgaa atggtaaacg cgaagataaa
     3481 cggaataccg gtgcatgtcg ccaagggcac tacgatcctg gaggccgccc ggaaggtgaa
     3541 cgtcaagatc cccaagctct gccacaacaa cgacctggaa ccctgggcgg cctgcggcat
     3601 ctgcgtggtc aagaacggca agagccccaa gatgctccgg gcctgctgca cccccctcga
     3661 ggacgggatg gacatcatca ccaacgacgc ggagctggtg gccacccgcc gcaccgtgat
     3721 cgagctgatc ctatccaccc acccggacga ctgcctccag tgcccccgca acggggactg
     3781 cgagctccag accctggcgg cggagttcgg catccgggag cagtccttcc ccaagcgcct
     3841 ccggggcctg gccacggacg agtccacggg caccatcatc ctcaaccccg agaagtgcat
     3901 ccggtgcgga cgctgcgtga aggtctgcca ggacatgcag ggcgtctggg ccatcgaatt
     3961 tctgggccgc ggggagaaga cccgcatcgc cccggccgcg gacgtggccc tggcggaagg
     4021 cccgtgcatc aagtgcggcc agtgctccgc ccattgcccc gtgggagcca tctacgagat
     4081 ggaccagacc aaggtcgtct gggacgccct catgaaggaa ggccccgacg ccaagacctg
     4141 cgtggtccag atcgcccccg cggtccgggt ggccctgggc gaggccttcg gcctggaacc
     4201 ggggacggtc atcaccaaga agatctacgc cgccctccgg cgcctgggct tcgatgccat
     4261 tttcgatacg agcttttccg cggatctcac gatcatggag gagggcacgg agttcgtgaa
     4321 gcggttcacc accgacgacg gccccctgcc cctgataacc tcctgctgtc ccgcctgggt
     4381 ggactacatg gagaagtact acccggatat gataccgaac ttctcgaccg ccaagagccc
     4441 ccagcagatg atgggcgcca tgatcaagac ctactgggcc gaaaaggcct ccacggatcc
     4501 gtccaagatt ttctccgtct ccatcatgcc ctgcacggcc aagaagtacg agaacagccg
     4561 caacgacgac atgtactcct cgggccagaa ggacgtggac gtaaccctga ccacccggga
     4621 actggcccgg atgatcaagc aggcggggat cgacatcctc aacctcccgg acgaggagcc
     4681 ggactccccc atggggccct acaccggggc gggagtcgtc ttcggcgcaa ccggcggcgt
     4741 catggaagcc gccctgcgct ccgcgtacta cctgatcacc gacgaggacc tcaaggacgt
     4801 gaacttcacc ggcgtccggg gcatctccgg cgtcaaggaa gcccgggtcc acatcaaggg
     4861 aaccgacgtc cgcgtggcgg tggcccacca gatgggcaac attgccatgg tcctggagga
     4921 ggtccggaag gccaaggccg agggcaagga ggttccctgg cacttcatcg aggtgatggc
     4981 ctgccggggc ggctgcatcg gcggcggcgg acagccctac ggctgcaccg acgaggtgcg
     5041 ccagaagcgg atggcgggga tctacgcgga cgacgagaaa agcgaatacc gctgttccca
     5101 ccgcaacccc tacatcggcc aggtgtacaa ggagttcctg gagaagccgg gttcccacaa
     5161 ggcccacaag ctcctgcata cccattacac ccggaagccc gtgtacaagt gagacccgcg
     5221 ccgcagggcg cggagtttcc ggaggggcgt cccgcggggg acgcccctct gctatgggga
     5281 gcccggtgcc ggcccggagc ggtcccggcg cgaccggggg agatagaatt tatggaggac
     5341 atcgatttcc gggcctcggc acggacggtg ctggaggaga cggaccggct ggtccgccgg
     5401 tacggtcccc ggctggccgg gtcggaggcg tgccggtccg tcgcccggga gctcgcggcc
     5461 cggctggaag gcttctgtga ccgggtccgg ctggaggagt tccgggcgca tcccgcctcg
     5521 ttctacgcct acaccaagat cctgcccctc gtgtacgtac tggggctggt ctcgcttctc
     5581 ctgaaccggg gtttctacct ttttccggcc ctgggcatcg ccgggggcat cgccctcatg
     5641 gtctcccagt tcgcggtcta cgggaggctt ggggacgggc tcttcccggc ccggacctgc
     5701 gtcaacgtag agggcgtcgt cgagcccgag gggaaggcgg aatacgagct cctcttgtcc
     5761 ggccaccatg acagcgcccc ggtggcgcgc atctattccg gtcctttcca gaaattctac
     5821 gccgccgcca tcttcgcccc gtaccccttc ttcctcctcg agctcgggct cctggcggcc
     5881 cgggccgcgg gctggatccg ggccgtcccg gccgggtggt ccgccgccct cctggcggga
     5941 cttcccttcg cggcggcgta cttcctcatg atcgataccc ggcgcgggag cccgggggcg
     6001 ggggacaacc tcgtgtcgtc gttgctctgc gtggagatcg cccgggaact ggccgcccgg
     6061 aaaggcgacc tgctccggcg aacccggatc cgggtgctga gcttcgacgc cgaggaggcg
     6121 ggtctccggg gctccgcggc ctacatgcgg tcccgggccg gggagatccg ggatcgggcg
     6181 gcgtggatgc tcaacttcga ttccctctac gacgcggccc acctgcacgt gctggatcgg
     6241 gacgtgaacg ggttcctgcg gctgtcccgg gaggtcgcac aggacgccgt cgaatgtcta
     6301 cgtgccgacg gatacaaggc gagggtcttc cccatggtgt tcgggggcgg gggaaccgac
     6361 gccgcggaag cggcccgggc cgggattgag gcggccacgg tgatagccat gccgacggcg
     6421 gtcgtccggg aggggctcgt ataccatacg ccgagggata ccccggacgc ggtcggtgtc
     6481 gacgcggtcg aagcctgcct tcgtctcacg gcccggtatg ccgggcggat cgagtcgggg
     6541 acgtcccggc gccgtaaagg cggcgtacgg tagttctccg agcgtcggct cggtgccgcc
     6601 ggtatcggaa acatcgccga acgtcagttc ggcgccatga gggcggcgta cggtagttct
     6661 ccgagcgtca gctcggtgcc gtcggtatcg gaaacatcgc cgaacgtcag ttcggcgcca
     6721 tgagggcggc gaagccgccc tcatgctatt tgggagcggc gatctttatg acgatctcct
     6781 ccccctcctc caccttcttg agggagttga tcaccgccag gctcacgtcc cgcacgaggc
     6841 ccttcacgaa ggggttcatg ggcacgttct tgcccccgca ggtcacggtc acgtctcgtt
     6901 ccattcgatc ctccaacagc ttgatgaatt cgtcgatatc gtccggagcc aggctcctca
     6961 cccccgcccc gacggcccgg gcggccagac ccgggtctcc ggtgatgagg acgtccacgc
     7021 gttccagggg caccttcagg tccgcctccg aggccgccgc cccggccgcc aggactcgga
     7081 gggccccgtc gacctccccc ccctcgacca ggatcacccg ggcatccggg aagagccggt
     7141 ccaggaaggc gcggtccagg gtcccggggc tccgcagggt caggaccgtc tccgggcccc
     7201 ccaccagggc cgtctccgcc ccggcctcga agaaccgggt cgagtccttt ccgggtatca
     7261 gaaagcttcc ggggtgcttg gtcaccttca gggccccaca gggtagtccc cgggcggaca
     7321 gggcacagat cgcgccctcg atgaaggtgg tcttccccgt gttggaccag ccgaaaaaag
     7381 aagcgacggt cttggagctc atacgacggg aataataggg gattgcggcg gcccgggcaa
     7441 ggcgctagga tgggcgcatg cgtacccgtc gaccgattct tcgagccgcc ccgtccctcg
     7501 ccgggctttc cgtacccgcg gtgctggcgc tcatcctggc cgcctgcgcc tccgggggcg
     7561 ccgtgaagtc aggtctctct gccgtttacg ccccgggtct gacggtaacc ctggtgtcct
     7621 ggaaggacgg ccaggccgag tacgccggaa acccggcctc cgggcgatgg ctgaccgtgg
     7681 acgcgcggat cgactacgac gggaacgccc cctccgcgga gctgcccgcc ttcgccctgc
     7741 gggtgcgatg ggaagccccg gacgggagcc ggagcgaatc cgctccgctc tgggtctacg
     7801 acccgacccc gggggccggg ggcgcggcca aacgggactc cctgcgggta aggatctcca
     7861 accgcttcat cctggcctac gacctgcccg tggacgcgat tcccctgtcc ttcgcggtgg
     7921 gcggtacgga cctgcccctg accgcgccct cgccgtagac ccttcaagcc ccgggcagga
     7981 cttcgatgcg ggtggccggc cccaggcccc agccgctctc ccggatctcg ggattctcca
     8041 cgcggatcac gtcccccctg cgaaggtagg aaaaccccga cgtccgcgac cgcggatcct
     8101 ccgcgggggc ggaaaagggg aaccgtcccg cagccccgca caggagatag cgcatccgtc
     8161 ccgccttgtt gagcagggga tccgaaacga cccggaagga gtcctccgga tacgcggacc
     8221 ggggcagggc ggcgggcgcc gagggcggcc gggccaggaa ccaggtcgcc ttcagggagt
     8281 ccttgtccag gccggtgcgc ttggccagga cccggaccgt ctccggggtc tcccatctca
     8341 gctcgtcgtg gcaggtgtgg gccggcccgg cggccagggc cggacagccc aggcgggacc
     8401 gacccgggca gggcgcgaag acggaccagc ccgcggcggc caggccgtcc cggagggcca
     8461 gggcgtcccg gctggtggcc aggagggcgg gctcgacgac caggaggaat ccttccgtgg
     8521 agagacagcc cgaggcggcc gccgccaggg ccaggacccg ctccgggcgg tcgtgcgcgc
     8581 cgatccacag ctcgttcagg gagtgtccga acacgacgca gtcgtaggga ccggcgtccg
     8641 cggcaccccc gtagggagcg ggtccttcca ggtccgcggc aaggtaccgg aggcggtcgg
     8701 aggatgcccg gccctgggcc aggacccggc gggccaggtc cagggcggag gagctggagt
     8761 ccacgaggac cgcggatacc gcgccggcgt ccaggagggc ggcggtcgcg gggcccggcc
     8821 ccgagcccat gtccaggacc cgggcgggcc ggaggcccga ctgggccagg acccccgcga
     8881 cctgggcgta ggatacgggc cagtagaaga agaggtaggc cgccaggagg tccgggtcgt
     8941 ccatgtaccg ggctccgatc agggaacggt tgccggtcag gccgcgctgg aggcggaaga
     9001 gggcgtcgga catccgggcg tactccgagt gggtcagccc ctcggacgat cccggttctc
     9061 ccgggcgccc ccggggcagt ccgaccagct cgtgccgcct tttaaggagg gcctcgacgc
     9121 gggaggcaag ggggttttcc ggggatcggg aatccggcat gggcctagga tatccctgcc
     9181 tcggcggtgc ggcaagggcc gctaagacgc ccggggcagg ggaggcagga cgggatcgtt
     9241 gtcgtcggtg ctgggatcgt ccttgcctcc cagtccggcc aggaagagga tctggcagat
     9301 cgcggcgggc agggcttcgg gcagggcttc ccgccgggag ccgtccccgg ggacgatccc
     9361 ctcgagttcc gccccgaagt cggacatgag ggggggcgta ccgaagaggt cttccagggc
     9421 cgggccctcc caggaacggg tgccggaccg ggggggcggc aggccgggca tctcccggca
     9481 aagggagtag gagggcgatc ccgtacgggt tcgaacgccg gagaaggcga agaggcggca
     9541 gatgaggggc cggccggggt agaccgtgca gtgggcgtcc gggcggtccg gatcgtagag
     9601 ggggcagccg cggaggttcg agggcagggt gccggaatcg gccatgcgca gggcgcgctc
     9661 gggacggttc cgcacgagcc aggcggccag gtaggaggct tcgaggggta aaatgtcggg
     9721 gatgaatcct tcgcagcacc ttccgcagcc gggcgggcag gaaagtccgt tacgggcgcg
     9781 gaaggaggcc gaggcctcct ccgcgcggat gtaaatactg tgaaccgagg ttaggcggtc
     9841 gaagaggaca ccgaaacgga gcccctcggg caggatggtg tcgagcacgt tgttgttccc
     9901 cgtggggcac ccgcattctg ccggaaatgg aggttttagt caatcctccc ggcggagagc
     9961 tccgagcggg ccgcctcccg ggcggcctcc gccgtctttt tcggcacccg ggctttctcg
    10021 gcgatgtagg cgggctccgc ctcggccact gcccggaggg aaccgaaggc cgtaaggatc
    10081 cgggccgccc gtacggggcc caccccctcc gcggcctcga ggccggtcag gcgcagggtc
    10141 ttcccccgga gcctctggtt caggccggtg gcgaagcggt gggtctcgtc tcggacggcc
    10201 accaggaccc ggagggccgg ggagtccttg gacaggacga tcggcccctc ggcgccgggc
    10261 agccagatct cctcgttctc cttggccagt cccgccaggg gtaggtccgc ctccagggcg
    10321 tccaggatct ccttgaccgc gttgacctgg ccgatgcccc cgtccaccag gatgaggtcc
    10381 ggcaggtcct ccccctcgtt cagcaggccc gtgtagcggc ggcccacggc ctcgcgaagg
    10441 gcggcgaagt cgtcgatctt tccctccagg ctgcggatgc ggaaatggcg gtagttcttc
    10501 ttgtccggta ctccgttcga gaaggtaaca agggaggcga ccgtgtattt gccggacagg
    10561 tgggcgatgt cgaagccctc gatccgtgcg ggcaggactt ccaggtccag ggtccgccgg
    10621 agctcctcca gggcctccac gtccccggtc tccctccgcc gcttggccag gtcctccttg
    10681 gcgttgtgct ccgccatggc cagggcggcc tcgtggcggc ggtcttccgg ggcggccaga
    10741 tccgtttccg ccccgagctc ccggcggagg tactcccggg ccagttccat gccccgggga
    10801 tcccggaggt agatccgggg cggggggggg cgctccgcgg agtagtaggt ggtgaggaag
    10861 tccaggaccg cctcttccgg ggtgccgaag gcccgggacc ggaagagatc ccgtcccgcc
    10921 aggcgcccgc cccgcatggc gaagaccgtg aaggtcgcaa ggctcccgtc caatgcggcg
    10981 gccacgtagt cccggctctc ggggtcgaag tccaccaccc ggctctcgcc ccggaaggcc
    11041 tcgatggcct ggagggtgtc ccgggcccgg gcggcctcct cgaagc
//
LOCUS       DAOTEI010000116        11019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4196_length_11019_cov_5.659248, whole genome shotgun sequence.
ACCESSION   DAOTEI010000116 DAOTEI010000000
VERSION     DAOTEI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4196_length_11019_cov_5.659248"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /gene="thyX"
                     /locus_tag="P5313_11335"
     CDS             complement(<1..353)
                     /gene="thyX"
                     /locus_tag="P5313_11335"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRY80999.1"
                     /translation="MPHCIVPAAEEILDKEIPVLDKGFVRLVDYLGGDERIVQAARVS
                     YGAGTRTVREDSALIDYLLRNDHTSPFEQVVLTFHVKLPIFVARQWIRHRTARLNEIS
                     GRYSVMKDEFYLPGEG"
     gene            complement(386..1279)
                     /locus_tag="P5313_11340"
     CDS             complement(386..1279)
                     /locus_tag="P5313_11340"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRY81000.1"
                     /translation="MRANLHLHSRRSDGTLSPAAVVEAAAAVGLEAAALTDHDTLGGV
                     PEFLARAEALGLAAWPAVEIDVSDRETGYRSEILAYFPGGRFEGTRELLAEVLERRRE
                     RARLWMSRVPRVFSREDLSYEGFLRFKAGDPDGDAPEGEYSLSKTDLFEYLKAQGVLT
                     RAAEYREFRKAYFDTDLLPSGSYRKTELAEVVRTVRSDGGICVLPHPGHEFGDSLSEL
                     SRDRKGFRSLLRRFRDLGVRGVEIYHYGNPDSEGINRIAAEEARDLGLFLTFGSDCHG
                     PGSVKYTIRDFRGDFPGWPGD"
     gene            complement(1292..2689)
                     /locus_tag="P5313_11345"
     CDS             complement(1292..2689)
                     /locus_tag="P5313_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY81001.1"
                     /translation="MNGKLGIRAKLSYGLGDFGYSIMGQTVTMFLIFFFTDAFGIPPA
                     AAGTVMLISKIWDAVSDPMMGSIVDRTRTRWGSKRPWILFGAVPFGLTFFLLFLGPEL
                     SAPARVAYAGITFILYCTAQTVVNIPYSALTVSMTEDADERSSVTAWRMSLALVGTLI
                     AGAAAPVVVQAMGGGIPGYRRYALIAGAVGAAMYVITFLGVEEKNFGSEEKVEFLANL
                     RSVFVNRPFLVLAGSTLATMTALNILAATVPYYFSYNLRSPGLVAAGLGVLLGSAILF
                     MPLLGALSKRLGKARAYMLALAETTAVIVLLRYLGERSPFLALGLFALAGYGVGSMYL
                     YPWSLVADTIEYSQWKTGIRPEGALYGYFTFVFKVGTALSSFIIGVVLSAFRYVPNVE
                     QSASGLEGIRLLLSFIPAALFLAGIALLARYPIDGPTHARYVDEITRGVHPGASAASA
                     ASARAPGRTGTGTGN"
     gene            2731..4080
                     /locus_tag="P5313_11350"
     CDS             2731..4080
                     /locus_tag="P5313_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRY81002.1"
                     /translation="MKIRPWKPGILSDSRTKGPKPWFPALAALLAPAYMKLSLRIRSV
                     RVEGAEILGAEYDAARRGRSRFLVAFRHPGDADPHLAYWFLHLRLPRELGRPRRDFGG
                     RFVAGAEIPLWGGPLVRWALRSAGTVPVRHGSLDRPTLDTLVTSIAEDPRPVALAPEG
                     QVTYHAGTVQSLDEGVARLALWASERLAAAGRPLPVRIVPLAVEYRFTPRARSRLPAF
                     LSRLERRCGLEDGSGLAPRERLDRIWERLVILVEDHYARTYGQAPAPAGSGIRERLER
                     ILESSLARLEAFYGRSSPGPDTGGTAGLKARTLAARAAAMDRVFHTPARWEGMSPVER
                     GMARRTAAEAFFLDRHQQLVDLGEYLDPAYAGSEGSETPPDRLIEIAQNLWDLANRLE
                     GGDIASRSRDFRKDVVLRVGAPVDASRREGEGSRAAALRVLSDLRRGFEALADASSQ"
     gene            complement(4074..4640)
                     /locus_tag="P5313_11355"
     CDS             complement(4074..4640)
                     /locus_tag="P5313_11355"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HRY81003.1"
                     /translation="MPILLSAILALSFSSCMPRRVRLDRDIPVYLQPGMPSGFEGAIR
                     DCLAIWNREFGTGLRIAGYHEGGQARDGRNTISLGVAAPPSPARRRRDARLEADRLGF
                     TVNWRRGLRIDETDILINISWRVAPRAVLEPEDYCLETLVLHELGHMFGLPHQDREGS
                     LMYPLLEPGRSVLAVDDRMRAEFSRRYY"
     gene            complement(4716..6995)
                     /locus_tag="P5313_11360"
     CDS             complement(4716..6995)
                     /locus_tag="P5313_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY81004.1"
                     /translation="MSMSDLDPSAVGGDETIPLLDLPPEDSLFSEALMSLLRTCRKPG
                     GELDADFQILFDGSPDMLVLIGPDGVFRDANPSFLGTSGYDIRQLRGRSILEILEAGY
                     RDGFREKLEAIGRARAAPGEAPPREIFAFRGLKPDGSTASMECLLVPILLQGKAVVLA
                     AIRNLTLERRLLEELRESEEQYAALSETISEAIFRIGEDLVILFANSGAMSTFGYARD
                     ELVGQNFQILFPEEVFRTYEDVFRKYFYVDDKDRPAMGLRRTLEVLGSTKNRGVAPME
                     MSFGNSKVFKERTLTCIVRDISHRKNLERKLRHLAYHDRLTGLANRELFHEDLQAALA
                     GLEKDKTRKHAVLFMDLDGFKQINDTLGHDRGDLLLVEAARRIRDSLRDADTAYRLGG
                     DEFTALLSGIRKVQDALIVAERIQSSIRQPFFLEGTEDAPISVGVSIGISVVPDDGTT
                     VQEVTKCADIAMYRSKDSGKGTCTVYDPAMDARVAERWRMAQEIRTALVRGEFRLYYQ
                     PIVDQNGMIRGMEALLRWPREDGTSIPPSVFVPVAEESGTIFLLGKWVLHRALADLRV
                     LQDRNLRDVYVAVNLSGRQFEQTGFADSLVDVVRSSGVDPGRLRLELTETTLMRNPAA
                     AAKRIQGIKNALPGLRFMIDDFGTGYSSLAYLSNLPVDSLKIDLSFVANLSRTQNEKV
                     VKAIINLGHSLEMGIVAEGIETKDQRSYFVERSCQGMQGFYFMKAAPLPNVLKRLERQ
                     LQGIPPFQEEAGRAEAAGE"
     gene            complement(7132..7698)
                     /locus_tag="P5313_11365"
     CDS             complement(7132..7698)
                     /locus_tag="P5313_11365"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRY81005.1"
                     /translation="MDEPVSIEFAILGLLYEGPLHGYELYRRYAGRSGLSVVWKVKRS
                     RFYALLARLGRLGLVAMDVREQAGRPPRKVFRLTPEGQAALRAWMTEPVADSRAFRVE
                     FPAKLYFCARVDPPAARRLVSEQKAVCDAWLRLRRLESGDRGFEALIRRFRTGQLAAM
                     TAWLSDCEAYLDGRTDPGAAGEIPGRPL"
     gene            7892..8938
                     /locus_tag="P5313_11370"
     CDS             7892..8938
                     /locus_tag="P5313_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY81006.1"
                     /translation="MGKFGKAIAILVLSSLTVGMAFANGTKDAAAPAGSTELQPGTYT
                     WVAGSMGGGWYTVAGGFARLVNEKEPKITIKVIPGGGVANPIALNNGDVDIAWGVGYV
                     DKAAFNGTAPIYDKAYKNLASISGTMAVDYYHFLAAKDTGVTTLDQFVDKIKKGEKIK
                     IAAPMTGTSDYVMTSFVLAYYGISYDTIKQNGGTVIQAIYGDMPSLYKDRHVDYAFAT
                     LGLPGAIMTEMAMGRPSTLMALSNEIIDHCAKTYGTVARASGLARVPGGTYTGMPNEI
                     QTLCHSTEILVSPSMPAAAVYAITKVLNENKDFLVSLGAGYRVFDPKTTWSTVQVPLH
                     PGAEKYYKDVGVMK"
     gene            9045..10952
                     /locus_tag="P5313_11375"
     CDS             9045..10952
                     /locus_tag="P5313_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRY81007.1"
                     /translation="MRELKGHLKVLVSIWAAAIAVFHLYTAVFGIMQPRIQRGIHILF
                     LLPLAFLLYPATKKSPTDRPSMPDFFLAFLATLPSLYVIVMNEPLNMRMPMVSPMTTL
                     ELWLGIVNILLLIEAIRRVVVPAMAVLVLVFFAYIFIAPYLPGIFYSRRVSLARIVEM
                     NYLITDSGIYGAITGVTATFVAIFVIFGSFMQYTKTGEFFTNFACKVAGSGPGGPAKI
                     AVVSSGLFGSISGVAAANVYATGTFTIPLMKRLGYRPQFAGAVEAAASTGGLIMPPIM
                     GAGAFVMSEITNIPYATIALAAALGAILYYFSIGIRVHLIALKDNLKAMDPSEMISWK
                     QVLMDSYLLIPLVVLIALLVIGYSPFGACTYAIGASFLLGFLRKETTMTPKRLFDAFR
                     LSGFNLIMLAVSCAGAGMVISIVTYTGLALGIATVITSFSHGILLPALILVMITSIIL
                     GMGLPCTPAYIIAVTIGGPAMLGLGIEMLPAHLFVFYFAILAEVTPPVCIAAYCGAAI
                     AGTKPLSTGFEASLLAIMGYIIPFVFVYNQALLLRGPALEIIGVFLALLVTTTLSGAA
                     ITGFLFKPLGILGRALTAVGSLAGVLVAANAKLLATTGPQLALIGAALAGIAVFVIGN
                     RRALRARGFAV"
BASE COUNT         1806 a         3817 c         3597 g         1799 t
ORIGIN      
        1 ccctccccgg gcaggtagaa ctcgtccttc atcaccgagt accgtccgga gatctcgttg
       61 agccgggcgg tccgatgccg gatccactgg cgggccacga aaatggggag cttcacgtgg
      121 aaggtcagga ccacctgttc gaagggcgag gtatggtcgt tgcgcaggag gtagtcgatg
      181 agggccgagt cctcccggac cgtccgggtc ccggcgccgt aggacacccg ggcggcctgg
      241 acgatcctct cgtcccctcc caggtagtcc accaggcgca cgaaaccctt gtccaggacc
      301 gggatctcct tgtccaggat ctcttccgcg gcgggtacga tgcagtgggg catggcaaga
      361 cagtaccctg tccgaggtcc gggtctcaat cccccggcca gccggggaag tccccccgga
      421 agtcccggat cgtatacttg acgctgccgg gcccgtggca gtcggacccg aatgtcagga
      481 agaggccgag gtcccgggcc tcctccgcgg cgatccggtt gatgccctcg gagtcgggat
      541 tcccgtaatg gtagatctcc acgccccgga cccccaggtc ccgaaaccgg cgcaggaggg
      601 atcggaagcc cttccggtcc cgggacaact cggacaggct gtccccgaac tcgtggcccg
      661 gatggggcag gacgcagatc cccccgtcgg agcggacggt ccggacgacc tcggccaact
      721 cggtcttccg gtacgagccc gagggcagga ggtcggtgtc gaagtaggcc ttccggaact
      781 cccggtactc cgcggcccgg gtcaggaccc cctgcgcctt gaggtactcg aagaggtccg
      841 tcttgctcag ggagtactcc ccctccgggg cgtccccgtc gggatccccg gccttgaacc
      901 ggaggaagcc ctcgtagctc aggtcctccc gggagaagac ccggggtacc cgggacatcc
      961 agagccgggc ccgttccctc cggcgctcca gcacctccgc cagaagctcc cgggtgccct
     1021 cgaaacggcc tccggggaag taggccagga tctcgctgcg ataccccgtc tcccggtccg
     1081 agacgtcgat ctcgaccgcg ggccaggccg cgaggccgag ggcctcggcc cgggccagga
     1141 attccgggac gccccccagg gtgtcgtgat ccgtcaaggc cgcggcctca aggcccacgg
     1201 cggccgccgc ctccaccacg gccgcggggg acagggtccc gtcggagcgc cgggaatgca
     1261 ggtggaggtt ggcccgcatc gaggcggctc cctagttccc ggtgcccgtc ccggtccggc
     1321 ccggggcccg ggcggacgcg gcggacgcgg cggacgcccc gggatggacc ccccgggtta
     1381 tctcgtccac gtaccgggcg tgggtggggc cgtcgatggg ataccgggca aggagggcta
     1441 tcccggccag gaagagggcc gcggggatga aggagagaag caggcggatg ccttcgaggc
     1501 ccgaggcgga ctgctccacg ttggggacgt accggaaggc cgaaagaacc acgccgatga
     1561 tgaagctcga cagggccgtg cccaccttga agacgaaggt gaagtacccg tacagggccc
     1621 cctcggggcg gatcccggtc ttccactggg aatactcgat ggtgtccgcg acgagggacc
     1681 acgggtacag gtacatggac cccaccccgt agcccgccag ggcgaagagc cccagggcca
     1741 ggaaggggct ccgttccccc aggtaccgga gcaggacgat gaccgccgtc gtctccgcca
     1801 gggccagcat gtaggcccgg gccttgccca agcgcttgga cagggccccc aggaggggca
     1861 tgaaaaggat ggcgctgccc aggaggacgc ccagacccgc ggccacgagg cccggggatc
     1921 tcaggttgta gctgaagtaa tagggtaccg tggcggccag gatgttgagg gccgtcatgg
     1981 tggccagggt ggagcccgcc aggaccagga agggcctgtt cacgaagacg ctccgcaggt
     2041 tggccaggaa ctcgaccttc tcctcgctcc cgaagttctt ttcctccaca cccaggaagg
     2101 tgatgacgta catggccgcg cccacggccc ccgcgatcag ggcgtacctc cggtatccgg
     2161 gaatccctcc ccccatggcc tggaccacca cgggggcggc cgctccggcg atcagggtgc
     2221 ccaccagggc cagggacatc ctccaggccg tcacggagga gcgctcgtcc gcgtcctcgg
     2281 tcatcgacac ggtcagggcg ctgtagggga tgttgaccac cgtctgggcc gtgcagtaga
     2341 ggatgaaggt gatccccgcg taggccaccc gggcaggtgc ggaaagctcg ggccccagga
     2401 acagaaggaa gaaggtcagc ccgaagggta cggccccgaa gaggatccag ggccgcttgg
     2461 aaccccagcg ggtccgtgta cggtcgacga tggatcccat catgggatcc gaaacggcgt
     2521 cccatatttt ggagatcagc atcaccgtcc ccgccgcggc ggggggaatg ccgaaggcgt
     2581 cggtgaagaa gaagatcagg aacatggtca ccgtctggcc catgatgctg tacccgaagt
     2641 cccccaggcc gtaggagagc ttggcgcgga tgccgagttt tccgttcatg gaccgcatga
     2701 tacactgact tccgaaagga cgccagaacc atgaagatac gcccctggaa acccggcatc
     2761 ctgtcggact cccggacgaa aggccccaag ccctggttcc cggccctggc cgccctcctg
     2821 gcgcccgcgt acatgaagct ctccctgcgg atccgctccg tgcgggtcga gggggcggag
     2881 atcctgggcg ccgagtacga cgcggcccga cgcggccgat cccggttcct ggtggccttc
     2941 cgccatcccg gggacgcgga cccccacctg gcctactggt tcctccacct ccggcttccc
     3001 cgggaactcg gccggcctcg gcgggacttc ggcgggcgct tcgtggccgg cgcggagatc
     3061 cccctctggg gcggccccct ggtccgctgg gcccttcgga gcgcgggtac cgtgccggtc
     3121 cgccacggca gtctggaccg gccgaccctg gacacccttg ttacctcgat cgcggaggat
     3181 ccgcggcccg tggccctggc cccggagggc caggtcacct accacgccgg cacggtgcag
     3241 tctctggacg agggagtcgc ccgcctggcc ctctgggctt ccgagcgcct cgccgccgcg
     3301 ggccgccccc tgcccgtccg gatcgtcccc ctggccgtgg agtaccgctt caccccccgg
     3361 gcccggtccc gcctcccggc cttcctgtcc cgactggagc ggaggtgcgg cctggaagac
     3421 ggttccggcc tggcgccccg ggagcgcctg gaccggatct gggagcgcct ggtgatcctc
     3481 gtggaagacc actacgcccg gacctacggc caggcccccg ccccggcggg ctccgggatc
     3541 cgggagcgcc tggagcggat cctggaaagc tccctggcgc ggctggaggc cttctacggc
     3601 cgttcctccc cgggcccgga cacgggagga acggcgggac tcaaggcccg gaccctggcc
     3661 gcccgggccg cggccatgga tcgggtcttc cataccccgg cccgctggga gggcatgagc
     3721 cccgtggagc ggggcatggc ccggaggacc gccgcggagg ccttcttcct ggaccggcac
     3781 cagcagctcg tggacctggg ggagtacctg gatcccgcct acgcggggtc cgagggttcg
     3841 gagacgcccc cggaccgcct gatcgagatc gcccagaacc tctgggacct ggccaaccgc
     3901 ctggagggcg gggacatcgc ctctcgcagc cgggacttcc gcaaggacgt ggtcctgagg
     3961 gtcggggccc ccgtggacgc gtcccgccgg gagggcgagg gaagccgggc cgcggccctg
     4021 cgcgtccttt cggacctgcg ccgggggttc gaggcgctgg ccgacgcttc ctctcagtag
     4081 taccttcggg agaactccgc acgcatccgg tcgtccaccg ccaggaccga ccggccgggc
     4141 tccaggaggg gatacatcag ggaaccctcc cggtcctggt ggggcagccc gaacatgtgc
     4201 cccagctcgt ggaggaccag ggtctccagg cagtagtctt cgggctccag gacagcccgg
     4261 ggcgccacgc gccaggaaat gttgatgagg atatccgtct catcgatcct caggcctctc
     4321 cgccagttta cggtgaaacc gaggcggtcc gcctccagcc gcgcatcccg tctccggcga
     4381 gccggggacg gaggcgcggc cacccccagg ctgatcgtgt tccggccgtc ccgggcctgc
     4441 cctccctcgt ggtaccccgc gatccgaagc cccgtcccga attcccggtt ccatatcgcc
     4501 aggcaatccc ggatcgcccc ctcgaagccg gacggcatgc ccggttggag gtagaccggt
     4561 atgtcccggt ccagccggac gcggcggggc atgcaggagg agaaggagag agcgaggatg
     4621 gcggagagga ggatgggcat caagaaacgt ccgcggcgat ccatcgcgat tcccgtggag
     4681 ccgatcccgg ggtcgtcccg ggagccttcc ggcccctact ccccggccgc ctcggcccgg
     4741 ccggcctctt cctggaaggg cgggatgcct tggagctggc gctccagccg cttgaggacg
     4801 ttgggcaggg gggcggcctt catgaagtag aagccctgca tgccctggca ggaccgctcc
     4861 acgaagtagg acctctggtc cttggtctcg atcccctccg ccacgatccc catctccagg
     4921 ctgtggccca ggttgatgat ggccttgacc accttctcgt tctgggtccg ggaaaggttg
     4981 gcgacgaagg aaaggtcgat cttgaggctg tccacgggca ggttcgatag gtaggccagg
     5041 gaggagtagc cggtgccgaa gtcgtcgatc atgaaccgca ggcccggaag ggcgttcttg
     5101 atgccctgga tgcgcttggc cgccgccgcg ggattccgca tcagggtggt ctccgtgagc
     5161 tccaggcgga ggcgcccggg gtccaccccg gaggatcgca ccacgtccac cagggaatcc
     5221 gcgaaacccg tctgctcgaa ctggcggccc gagaggttca ccgccacgta gacgtcccga
     5281 aggttccggt cctggagcac ccgcaggtcc gccaaggcgc ggtgcaggac ccacttcccc
     5341 aggaggaaga tggtcccgga ttcctcggcc acgggcacga agaccgaggg cggtatgctc
     5401 gtcccgtcct cccggggcca ccggagcagg gcttccatgc cccggatcat cccgttctgg
     5461 tccacgatgg gctggtagta gaggcggaac tccccgcgga ccagggcggt ccggatctcc
     5521 tgggccatcc gccaccgctc cgccacccgg gcgtccatgg ccgggtcgta gaccgtgcag
     5581 gttcccttgc cgctgtcctt ggaccggtac atggcgatgt ccgcgcactt ggtgacttcc
     5641 tggaccgtgg tcccgtcgtc gggaaccacg gagatcccga tgctcacccc cacgctgatc
     5701 ggtgcgtcct cggtaccctc caggaagaag ggctggcgta tggaggactg gatcctttcc
     5761 gccacgatca gggcgtcctg gaccttccgg atcccggaga gcagggcggt gaactcgtcg
     5821 cccccaaggc ggtaggccgt gtccgcgtcc cggaggctgt cccggatccg ccgggcggcc
     5881 tccaccagga ggaggtcgcc ccggtcgtgt cccagggtgt cgttgatctg cttgaacccg
     5941 tccaggtcca tgaagagcac cgcgtgcttc cgggtcttgt ccttttccag acccgccaag
     6001 gccgcctgca gatcctcgtg gaagagttcc cggttggcca ggcccgtgag ccggtcgtgg
     6061 taggccaggt gacggagctt gcgttccagg ttcttccggt gggagatgtc ccggacgatg
     6121 caggtcaggg tccgttcctt gaacaccttg gaattcccga aggacatctc catgggggcc
     6181 acgccccggt tcttggtgga ccccaggacc tccagggtgc gccgcaggcc catggccggc
     6241 cggtccttgt cgtccacgta gaagtacttg cggaagacgt cctcgtaggt gcggaagact
     6301 tcctcgggaa agaggatctg gaagttctgc cccaccagct cgtcccgggc gtacccgaag
     6361 gtactcatag ccccggagtt ggcgaacagg atgaccaggt cctcgccgat ccggaagatc
     6421 gcctcggaga tcgtctccga gagggccgcg tactgctcct cgctctcccg cagctcctcc
     6481 aggaggcggc gctccagggt caggttccgg atggcggcca ggacgacggc cttgccctgg
     6541 agcaggatcg ggaccaggag gcattccatg gaggccgtcg agccgtcggg tttcagcccc
     6601 cggaaggcga agatctcccg gggaggcgcc tcccccggcg ccgcccgggc acggcctatc
     6661 gcctccagct tctcccggaa cccgtcccgg taccccgcct ccaggatctc caggatgctc
     6721 cgcccccgga gctgccggat gtcgtacccg ctggtgccca ggaaggaggg gttggcgtcc
     6781 cggaagaccc cgtccggccc gatcaggacc agcatgtccg gggaaccgtc gaacaggatc
     6841 tggaagtccg cgtccagctc ccctcccggc ttccggcagg tccggagcag gctcatgagg
     6901 gcctcggaga acagggaatc ctcagggggc aggtcgagga ggggtatcgt ctcgtctccg
     6961 ccgaccgcgg agggatccag atccgacatg ctcatcggtt tcctaaggtt ttcggcttcc
     7021 ccgagtcccg cggaggcctc ggggcgccgg agcccgttca ggacgaaatc cggccttatc
     7081 ttatcggcag gaacggctcc ctacttgaac gtatcgtgaa tcggcgtgaa ttcaaagcgg
     7141 acgtcccgga atctccccgg ccgccccggg atccgtccgg ccgtccaggt aggcctcgca
     7201 gtcggaaagc caggccgtca tggccgccag ttggccggtc cggaagcggc ggatcagggc
     7261 ctcgaagcct cggtccccgg actcgaggcg gcgaaggcga agccaggcat cgcacaccgc
     7321 cttctgttcc gacaccaggc gccgggccgc cgggggatcc acccgggcgc agaagtagag
     7381 cttggcgggg aattccaccc gaaaggcccg gctgtccgcc acgggctccg tcatccaggc
     7441 ccggagggcg gcctgccctt ccggggtcag gcggaacacc ttccggggcg gccggcccgc
     7501 ctgctcccgg acatccatcg ccaccaggcc gagccgtccg agccgggcca ggagggcgta
     7561 gaaccggctc cgcttgacct tccagacgac ggacaggccc gaccggccgg cgtagcgccg
     7621 atagagctcg tagccgtgga ggggcccctc gtacaggagg cccaggatcg cgaactcgat
     7681 ggataccggt tcgtccatgg ataccagtgt ccggcgggga tgccccgccg tcaagcgggt
     7741 atcccgttcc tggtctgctc cttgaagaac tttgtttctg catgtagtat cgcataaaca
     7801 tgcggagatt tcgggttgac aactccggag taccgtccta ccattactca tccgctgaag
     7861 agtagtggat gaatccaagc aggaggcacc aatgggcaaa ttcggaaagg ctatcgcgat
     7921 tctggtatta tcatccctta ccgtcggcat ggccttcgcc aacggaacca aggacgccgc
     7981 ggccccggcg ggttcgacgg aactccagcc cgggacctac acctgggtgg cagggagcat
     8041 gggcggcggc tggtataccg tcgccggagg attcgcccgg ctggtgaacg agaaggagcc
     8101 caagatcacg atcaaggtga tccccggcgg cggcgtggcc aacccgatcg ccctgaacaa
     8161 cggggacgtg gacatcgcct ggggcgtggg gtacgtggac aaggccgcct tcaacgggac
     8221 cgcccccatc tacgacaagg cctacaagaa cctggcctcc atctccggaa ccatggccgt
     8281 ggactactac cacttcctgg cggccaagga caccggggtc accaccctgg accagttcgt
     8341 ggacaagatc aagaagggcg agaagatcaa gatcgcggct cccatgacgg gaacctccga
     8401 ttacgtcatg acatccttcg tcctggccta ctacgggatt tcctacgaca caatcaagca
     8461 gaacggcgga accgtcatcc aggccatcta cggcgacatg cccagcctct acaaggaccg
     8521 ccacgtggat tacgccttcg ccaccctggg gctcccgggg gccatcatga ccgaaatggc
     8581 catgggccgg ccctccactc tgatggccct ctccaacgag atcatcgacc actgcgccaa
     8641 gacgtacggg accgtcgccc gggccagcgg gctggccaga gttcccggcg ggacgtacac
     8701 aggcatgccg aacgagatcc agaccctctg ccattccacc gagatcttgg tcagtccgag
     8761 tatgcccgcg gcggcggtct acgccatcac gaaggtcctg aacgagaaca aggacttcct
     8821 cgtgagcctg ggcgccgggt acagggtctt cgatcccaag accacctggt ccacggtgca
     8881 ggtcccgctc catcccggag ccgagaaata ctacaaggac gtcggagtca tgaagtaatc
     8941 atccgccgcc gcggaaggga atcgggcccg tacgggcctg atccccctcc gatccccgag
     9001 ccgcgcggac cctccgcatg aataccacga gataaggatt ccccatgaga gagcttaaag
     9061 gtcacctgaa ggtactcgtc tcgatctggg ctgcggcgat cgcggtcttc cacctctaca
     9121 ccgccgtctt cggcatcatg caaccgcgca tccagcgggg gatccatatc ctctttctcc
     9181 tgccgctggc cttcctcctc taccccgcca ccaagaaatc cccgacggat cgcccgtcga
     9241 tgccggactt cttcctggct ttccttgcga cccttccgtc cctgtacgtc atcgtcatga
     9301 acgaacccct gaacatgcgc atgccaatgg tctcccccat gacgaccctg gagctctggc
     9361 tgggcatcgt gaacatactc ctcctcatcg aggccatacg ccgggtcgtg gtgcccgcca
     9421 tggccgtgct ggtcctcgtt ttcttcgcgt acatcttcat cgcgccctac ctcccgggga
     9481 tcttctattc ccggagggta tccctggccc gcatcgtgga gatgaactac ctgatcaccg
     9541 actcggggat ctacggggcc atcacgggag tcacggcgac cttcgtcgcg attttcgtga
     9601 tcttcggctc cttcatgcaa tatacgaaga caggcgagtt cttcacgaac ttcgcctgca
     9661 aggtggccgg aagcggtccc ggcggacccg ccaagatcgc cgtcgtgagc tcgggtctct
     9721 tcggctccat ctcgggggtc gccgcggcca acgtctacgc cacggggacc ttcacgatcc
     9781 ccctcatgaa acgcctgggc taccgccccc agttcgcggg agccgtggag gccgccgcgt
     9841 ccaccggcgg actcatcatg ccccccatca tgggggccgg tgccttcgtc atgtccgaga
     9901 tcacgaacat cccctacgcg accatagccc tggccgccgc cttgggggcc atcctgtatt
     9961 acttcagcat cggaatccgg gtccacctga tcgccctcaa ggacaacctc aaggccatgg
    10021 atccatcgga gatgatctcc tggaaacagg tcctgatgga ctcctacctc ctcatccccc
    10081 tggtcgtcct gatcgccctc ctggtcatcg ggtactcccc cttcggggcc tgcacctacg
    10141 cgatcggggc ctccttcctc ctgggtttcc tacgcaagga aaccaccatg accccgaagc
    10201 ggctgttcga tgcgttccgg ctttcgggat tcaacctgat catgctggcc gtgtcctgcg
    10261 cgggggccgg gatggtaatc agcatcgtga cctacacggg cctcgccctc gggatcgcca
    10321 cggtgatcac gagcttctcc cacgggatcc tcctgcccgc cctcatcctg gtgatgatca
    10381 cctccatcat cctgggcatg gggcttccct gcaccccggc ctacatcatc gcggtgacca
    10441 tcggcgggcc ggccatgctg ggtctcggga tcgagatgct cccggcccac ctcttcgtgt
    10501 tctacttcgc catcctggcg gaggtgaccc ctcccgtgtg catcgccgcc tactgcgggg
    10561 ccgccatcgc ggggaccaag cccctgtcca cgggcttcga ggcctccctc ctggccatca
    10621 tgggctatat aattcccttc gtattcgtgt acaaccaggc cctcctgctc cggggccctg
    10681 ccttggagat cataggagtg ttcctcgctc tcctggtcac gacgaccctc tcgggagccg
    10741 ccatcacggg tttcctcttc aaacctctgg gcattctggg cagggctttg accgcggtcg
    10801 gaagcctggc gggggtcctc gtggccgcca acgccaagct cctcgcgacc acgggtcccc
    10861 agctggccct gatcggggcc gcattggcgg gcatcgcggt cttcgttatc gggaaccgcc
    10921 gagccctgcg ggcccggggc ttcgcggtct gagggccgcg gatcgaggat gcccgggatg
    10981 acgattcgag cggatgtcac ggtgctgggc gccggcgtg
//
LOCUS       DAOTEI010000117        10948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4238_length_10948_cov_5.018085, whole genome shotgun sequence.
ACCESSION   DAOTEI010000117 DAOTEI010000000
VERSION     DAOTEI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_10948_cov_5.018085"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1385
                     /gene="ffh"
                     /locus_tag="P5313_11380"
     CDS             42..1385
                     /gene="ffh"
                     /locus_tag="P5313_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRY81008.1"
                     /translation="MLERLTQKFGDVLRTIGGKSSISEKNIEEAVEQIKLALLEADVN
                     LRVVRRFVNSTIEEAKGEKVLRAVSPGQQFVKIVHDKMVALLGDTRQELQLRGPDVVS
                     VILLLGLQGSGKTTTAAKLAHHLKGKGRKVLLAACDLVRPAAVEQLSVLGSQIGVEVY
                     KEDGAKDPVKVARAALDRAKKGLFDTLIVDTAGRLEIDEAMMDQLVRIRDAVKPDEAL
                     LVADAMTGQAAVDIAKVFDEKVGVSGVILSKFDSDARGGAALSLKSVTGKAVKFIGVS
                     ERPDGLEPFHPDRIAGRILGMGDIVSLVEKAQEQFDAREAEELQRKMSEEGLTLEDYL
                     DQLRRMKKMGSLKGVLDMIPGAAGQISEDQIDEGELRREEAILTSMTRKERANYLIIG
                     PSRRSRIARGSGTSVAEVARLLKKFEKTRTMMKKMARGRGREAQAWAKMSVNGGR"
     gene            1566..1757
                     /gene="rpmB"
                     /locus_tag="P5313_11385"
     CDS             1566..1757
                     /gene="rpmB"
                     /locus_tag="P5313_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRY81009.1"
                     /translation="MARRCDICGKGTTFGHNVSHAKNRTNRVWKPNLVQVRTLIEGTV
                     LTVKVCTRCLKAGKIVKYV"
     gene            complement(1906..2373)
                     /locus_tag="P5313_11390"
     CDS             complement(1906..2373)
                     /locus_tag="P5313_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81010.1"
                     /translation="MKRAIIVVLAVAVAASAVSAQGWAPGFRGPGPGAYAAPAAPAET
                     VKIEGKLALVNAHPAVVVKDKTYYVGIPGRLFGFVDGLKEGAQVKLEGYEQPLPYATN
                     VAMFHVVKLTVGGREIDLSQSFGYGRGMMGAYGPAGDYGPMGRGMMPGGRGRW"
     gene            complement(2706..3581)
                     /locus_tag="P5313_11395"
     CDS             complement(2706..3581)
                     /locus_tag="P5313_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRY81011.1"
                     /translation="MSFLDALLDPALPFVRNAFLAGILSSVLFGVLGSVVTVKRIAGL
                     AGAISHAVLGGIGLALFLSARGLAPGLPPIAGALAFAVLSAALIGVVSLKAKQREDTV
                     INAIWAVGMSVGVLFIAKTPGYADPMSYLFGNILLISGRDLVLMAVLSALVVAGAWRF
                     YPQIEASAFDEEFARTRGVRTDAVFLAVLAVVAVSVVLLQTFVGIVMVIAMLTLPAGT
                     AGFAARNLRGMMVLASLLSAVFSVGGLALSWETDLPSGAVVVVLSGAVFLGASGIRML
                     VTRTSRPKAERPDGK"
     gene            complement(3584..4420)
                     /locus_tag="P5313_11400"
     CDS             complement(3584..4420)
                     /locus_tag="P5313_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81012.1"
                     /translation="MTANRDRVASALVPGSPHGHGRPIAVRFEGVSFAYGSDPILEDV
                     SFHIHEGEFAALIGPNGSGKTTILRLLLGLAAPASGRIEVLGAPPHTALESLGYVPQN
                     AAFDPAFPISVAEVVRMGCLRGGSSRYSREDLRAADAALEQADVAELRDRPYSALSGG
                     QRRRVLVARALAANPRLLVLDEPTANMDAESETRLFRALGELRGRTTVLVVTHDTLFV
                     SDLTDAVLCVGDRDGRPGSVVRHASIPVARGLAPGVFGGGARQVLHDTEIPPEACCGE
                     GD"
     gene            complement(4510..5430)
                     /locus_tag="P5313_11405"
     CDS             complement(4510..5430)
                     /locus_tag="P5313_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRY81013.1"
                     /translation="MRSSEPRTPFFLRAAALAVLTLAGAAAAAQAPRVAVSILPQKYF
                     VDRISEGGARVLVLVGPGQNPHSYEPTPRQMADLGAAGAWLTIGVDFEKGLLPKARNL
                     YPRLRLLDTTEGLRFRALEAHSHEEDEDSHEEAGEDGDHEGEGGRDVHVWLGREGAKA
                     QARHARDALSALDPGKAALYSRTHEALVRDIDAVFDDLAESLAPLRGRPVFVYHPAFG
                     YFLDEFGIEQVAVEAGGKEPTQKGLASLVEKARREGARVVFVQPQFSKAAARAVAQAI
                     GGVVVEIDDLAPDWLANLRKMGDALKAAAR"
     gene            complement(5528..6316)
                     /locus_tag="P5313_11410"
     CDS             complement(5528..6316)
                     /locus_tag="P5313_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY81014.1"
                     /translation="MRAVFLGTGTSHGVPVVGCPCPVCRSEDPRDSRYRASLLMRGDG
                     GERILVDPGPEFRLQALRAGIDRLDAVLVTHAHADHIHGLDDVRPLTREGPLPVWANR
                     EARAELEVRFSYAFDPPQRGGGVPRIRLPELPPEGVRIGGIRVLPVPVLHGILPILGF
                     RLGNFAYLTDCSGIPESSRPILKGVEILVLGALRERPHPTHFSISEAADAARSLGARE
                     AWITHICHDHSHEELEAICSGLSSPGFDLRPAFDGLELDIGPAS"
     gene            complement(6313..8619)
                     /locus_tag="P5313_11415"
     CDS             complement(6313..8619)
                     /locus_tag="P5313_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY81015.1"
                     /translation="MAKSNHGKTVPLLVLIMVPTVLSFLLAVGTVVLSSVQAGRKLVR
                     ESSISLTWKTAGEIDARLAGYFTAARSILSGLEAAVESGNLDLRDTASLRTLLYQFAG
                     ITPAASTLYYGDRSDRTVLVSRNADGGGLVCIRDETTGGKLNFYDLGQDGSVGAFREA
                     ADFAPTTRGWYKAAAESGEPGWTEIYVDFVSKGLVITPYVPVKDSSGAVVGVLGADVP
                     LDALKTLITEAAQGSGADAALLDAGGSLIALSRDFPVTRDVGGTPERIPGRESGDGVV
                     AAAAAHETVDAVSASSETSSTWYDEIRAGSGSWFISSSPLSKNVGIDGTILVYTSVEE
                     RMGRLYAALAGGGAVAAAAFLSGILIILVVTRRVSAQVRELRDVLTAVSTGDLSVSAV
                     KRSASEVGQIQAAVQALTDRLSAIVEDIQGAAEKSASAAETLAAHSSETAATITQMSA
                     GIASMKHQTEALDVAAGETEKARQEISSAAQTVLSAIRDLENAVEGTRGLIGEIGTEL
                     RTLAERASRQKETALRVSGLGADGRERVEAAASAMERMEASARRTLELVDIINGIAEQ
                     TGLLAMNAAIEAAHAGESGRGFAVVAEEIRKLSESTAENAQGISRTIEETAEAIRNAA
                     ESTETANGAIGSVIDGLEELMGELASTSDALQDAASRSREVVSALEGLAGTSQALSTA
                     SGGLESGTAVIGRTVEDVRRLSAENRSAAQEMTLGISEIDRSATELSDLSRQSADTTS
                     SIREAAAQFRTRGRTVTERGVDIKRDPA"
     gene            complement(8695..10050)
                     /locus_tag="P5313_11420"
     CDS             complement(8695..10050)
                     /locus_tag="P5313_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81016.1"
                     /translation="MTVRARMGFLLLAFVSALPLSAQVAPPLGGDLPLESIPESDDLR
                     VRWMAELIEAPRERALGFRTRTEENASGRWRLETIQGPRAFYIAVVPDREVLYGQGAW
                     IIKRDLSTGRFVQAKVFLRNDPNTFLRLYPFGDRTKLDLVIYRGVVRHDVVLPVPFRK
                     LLTSPLRDIVGWTRDSVDWELLSPVPGLYGNLRSLAQAVRERLGGLRYGDDGALDETG
                     RPVLIAGGVPAGDPGLNCSGFAKWVVDGMLKPVTGTWLSVEALKERHEDLRGSSFTQP
                     YEKARDPFFGLDWAWNLGKAVADALYPSRRNRVTDNDVRVPAFALVDRGSSEPANGSQ
                     TYEPYPANFEIGGYRVDGLRPLLYVLAIREPGNFYIASFNRRMGESVTLRQYFHEALL
                     FPYFDAGGEFRVAVFESAEETSLEAVLRRYPSEFAHLVRIPALPDFDPPALPVPSSAG
                     P"
     gene            10269..>10948
                     /locus_tag="P5313_11425"
     CDS             10269..>10948
                     /locus_tag="P5313_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81017.1"
                     /translation="MRSQLVRTALASLAAAAAAAAIFAEGGTSLGDPARPGLSGVPGT
                     ARSEPYRLFPRAYRPDPAPAVSAGPADHHVPDEAEYAAREAAARSQGQRVPVLANSDI
                     LSFYGHPTSRRMGILGEYPKDQLTSLLSGYAQLFDAANGDRDVIPAFHLIYGTCWPEG
                     EIGYLSSKTVEEYVRFAAERGMLVFLDHQIGKYTVEESVRRLLPFLRYPNVHLALDPE
                     WRTLSPMK"
BASE COUNT         1822 a         3658 c         3816 g         1652 t
ORIGIN      
        1 cgcggccgtc gcgccggcaa taccctttcc gcgaggaatc catgctcgaa cggctcaccc
       61 agaaattcgg ggacgtcctc cgcaccatcg gcggcaagtc gtccatcagc gaaaagaaca
      121 tcgaggaggc ggtcgagcag atcaagctgg ccctcctgga ggcggacgtc aaccttcggg
      181 tggtccgccg cttcgtcaat tccaccatcg aggaagccaa gggcgagaag gtcctccgcg
      241 cggtctcccc gggccagcag ttcgtcaaga tcgtccacga caagatggtc gccctcctgg
      301 gggacacccg ccaggaactc cagctccggg gccccgacgt cgtctcggta atcctcctcc
      361 tgggcctcca gggctccgga aagaccacga ctgcggccaa gctggcccat catctcaagg
      421 gcaagggacg aaaggtcctc ctggccgcct gcgacctggt gcgccccgcc gcggtggaac
      481 agctctcggt cctgggatcg cagatcggcg tcgaggtcta caaggaagac ggcgccaagg
      541 accccgtgaa ggtggcgcgt gcggccctgg atcgagcgaa aaaggggctc ttcgacaccc
      601 tgatcgtgga taccgcgggc cgcctggaga tcgacgaggc gatgatggac cagctggtcc
      661 ggatccggga cgccgtcaag cccgacgagg ccctcctggt ggcggacgcc atgacgggcc
      721 aggccgcggt ggacatcgcc aaggtcttcg acgagaaggt gggcgtgtcc ggcgtcatac
      781 tctccaagtt cgattccgac gcccggggcg gggcggccct gtccctgaag agcgtgaccg
      841 ggaaggccgt caagttcatc ggcgtctccg agcgtcccga cggcctggag cccttccatc
      901 cggaccggat cgccggccga atcctgggca tgggcgacat cgtctccctg gtggagaagg
      961 cccaggagca gttcgacgcc cgggaggccg aggagctcca gcgtaagatg tccgaggagg
     1021 ggctcaccct ggaggactac ctggaccagc tccggcgcat gaagaagatg ggctccctga
     1081 agggggtcct ggacatgatc cccggggccg cgggccagat ttcggaggat cagatcgacg
     1141 agggagagct ccggcgggag gaagccatcc tgacctccat gactcggaag gaacgggcca
     1201 actatctgat catcgggccg tcccgtcgct cccggatcgc ccggggctcc gggacctccg
     1261 tggcggaggt ggcccggctc ctcaagaagt tcgagaagac ccggaccatg atgaagaaga
     1321 tggcccgggg ccggggccgg gaagcccagg cgtgggcgaa gatgtcggtg aacggtggac
     1381 ggtgaacagt ggacggaagc gacacgatgg ttcggtcaga aatcccgcca aggcggggtg
     1441 tgtttccgca gtcttctgcc atttccgatc actgtccacc gaccaccgtc cacggaccta
     1501 ctttacactc cccgccctca tcgctatact acgcgaacca attcgcacaa ccggagaaga
     1561 ccgatatggc tagacgatgc gatatctgcg ggaagggcac caccttcgga cacaacgtgt
     1621 cccacgccaa gaacaggacc aaccgggtct ggaaacccaa cctcgtccag gtgcggacgc
     1681 tgatcgaggg caccgtcctg acggtcaagg tctgcacccg ctgtctgaag gccggcaaga
     1741 tcgtaaagta cgtctgatcg agcctcggac cgcgtgaaaa gccgcctttt aaggcggctt
     1801 ttttcttgtc ctcggtacgg cgggcccggg caaaaaagga ccccggccgg ggatcccggc
     1861 cggggtcgaa ccttcgttcg gcgtcgtcgg ggacgccgga gcggcttacc agcggccgcg
     1921 cccgccgggc atcatgcccc ggcccatggg gccgtagtcg ccggccgggc cgtaggcgcc
     1981 catcatcccg cggccgtagc cgaaggactg ggagaggtcg atctcccggc cgccgacggt
     2041 caacttgacg acgtggaaca tcgcgacgtt cgtcgcgtac ggcaggggct gctcgtaccc
     2101 ctcgagcttg acctgggcgc cttccttgag gccgtccacg aaaccgaaga gccggccggg
     2161 gatgcccacg tagtaggtct tgtccttcac gaccaccgcg gggtgggcgt tgaccagggc
     2221 gagcttgcct tcgatcttta cggtctcggc gggggccgcg ggggcggcgt aggcgccggg
     2281 gccggggccg cggaagcccg gggcccatcc ctgggcggac acggcggagg ccgcgacggc
     2341 gacggccagg actacgatga ttgcgcgctt catatggaac tccttgtggt tcactcagtc
     2401 ccgatcctca aggatcggtg ccggtttcct tccgacacct tatatattgc aatcgccgtg
     2461 ccaagtcgaa ttttttgaaa aaatcagaac aggattttca tgatgtacag gattaacaag
     2521 attaagaatc taaatattta tagaaatgga ataaaaatat aaacatgaga gagaataaga
     2581 atcatgttaa tctatatgaa atcatgatca tcctgttctg attatcctga gaaattggcg
     2641 tgatcaagcg cttttttagt atttcgtacg attgttccgg gaagtttttc caccccggtg
     2701 ggaaattatt tcccgtccgg acgctccgcc ttcggccggg atgtacgggt gaccagcatc
     2761 cggattcccg aggcccccag aaagacggct cccgacagga ccacgacgac ggctccggag
     2821 ggcaggtcgg tctcccagga cagggccagg ccgcccaccg aaaagaccgc ggacaacagg
     2881 gacgccagga ccatcatccc ccggagattc cgggccgcga accccgcggt tccggcgggc
     2941 agggtgagca tggcgatgac catgacgatg cccacgaagg tctggaggag caccaccgag
     3001 accgccacga cggccaggac ggccaggaag accgcgtccg tgcggacccc ccgggtccgg
     3061 gcgaactcct cgtcgaaggc ggatgcctcg atctgggggt agaagcgcca ggccccggcc
     3121 acgaccaggg cggaaaggac ggccatcagg accaggtccc ggccggagat caggaggatg
     3181 ttcccgaaga ggtagctcat ggggtcggcg tagcccgggg tcttggcgat gaacaggacc
     3241 ccgacgctca tgcccaccgc ccagatggcg ttgatgaccg tgtcctccct ctgcttggcc
     3301 ttgagggata cgacgccgat cagggccgcg gacaggaccg cgaaggccag ggctcccgcg
     3361 atcgggggga ggcccggcgc cagcccccgg gcggagagga acagggccaa gccgatgccg
     3421 cccaggacgg cgtgggagat ggcccccgcc agccccgcga tccgcttgac cgtgaccacg
     3481 gaaccgagta ccccgaagag gaccgaggac aggatccccg ccaggaaggc gttccgtacg
     3541 aagggcaggg cggggtccag gagggcgtcc aggaagctca tctttagtcc ccctccccgc
     3601 agcaggcttc gggagggatc tccgtgtcgt ggaggacctg ccgggccccc ccgccgaaga
     3661 cccccggggc caggccccgg gccaccggga tggaggcgtg gcgcaccacg gatccgggcc
     3721 gcccgtcccg gtccccgacg cagagtactg cgtccgtcag gtccgacacg aagagggtgt
     3781 cgtgggtgac cacgaggacc gtggtccggc cccggagctc gcccagggcc cggaagagcc
     3841 gggtctcgct ctcggcgtcc atgttggccg tgggctcgtc caggaccagg agtcgggggt
     3901 tcgcggcaag ggcgcgagcc acgaggaccc ggcgcctctg tcccccggac agggcggaat
     3961 agggacggtc ccgcagttcc gccacgtccg cctgctccag ggcggcgtcc gcggcgcgca
     4021 ggtcctcccg ggagtagcgg ctgctgccgc cccgaaggca acccatgcgc accacctcgg
     4081 ccacggagat ggggaaggcg gggtcgaagg cggcgttctg gggcacgtag cccagggatt
     4141 ccagggcggt gtggggaggc gcccccagga cctcgatgcg ccccgaggcg ggggcggcca
     4201 ggcccaggag gagacggagg atggtcgtct tccctgagcc gttcggcccg atcagggccg
     4261 cgaactcgcc ctcgtggata tggaaggaga cgtcctccag gatagggtcg gagccgtacg
     4321 cgaaagacac cccctcgaag cggacggcga tcgggcgtcc gtggccgtgg ggggatccgg
     4381 ggacgagggc cgaggcgact cggtcgcggt ttgcggtcat ggcatcccca tcctagaccc
     4441 tccgggtccg gagggcaagc gggcacggcc gtgtgataaa gcccgtgcag cgcagtaccg
     4501 ggcggcgcct caccgggcgg cggccttgag cgcgtccccc atcttccgaa ggttggccag
     4561 ccagtccggg gccaggtcgt cgatctcgac cacgactccc ccgatggcct gggccacggc
     4621 ccgggccgcg gccttcgaga actggggctg gacgaagacg acccgggcgc cctccctgcg
     4681 ggccttctcg accagggacg ccaggccttt ctgggtgggt tccttgcctc ccgcctccac
     4741 ggcgacctgc tcgatcccga actcgtccag gaaatacccg aaggcggggt ggtagacgaa
     4801 caccggcctg ccccgcaggg gggccaggct ctcggccagg tcgtcgaaga ccgcgtctat
     4861 gtcccggacc agggcctcgt gggtccggga gtacagggcg gccttcccgg ggtccagggc
     4921 cgagagggcg tcccgagcgt ggcgggcctg ggccttcgcg ccttcccggc ccagccagac
     4981 gtggacgtcc cggccgcctt ccccttcgtg gtcgccgtcc tcgccggcct cctcatggct
     5041 gtcctcatcc tcttcgtggg agtgggcctc cagggcgcgg aacctcagcc cctcggtggt
     5101 gtcgaggagc cgaagacggg ggtacaggtt ccgtgccttc ggaaggagcc ccttctcgaa
     5161 gtccaccccg atggtgagcc aggctcccgc ggcacccagg tccgccatct gccggggggt
     5221 cggctcgtag gagtgggggt tctggccggg tcctaccagg accaggacgc gggcgccgcc
     5281 ttcggagatc cggtccacga agtacttctg gggcaggatg ctcacggcta cccggggggc
     5341 ctgggccgcc gcggccgcgc cggccagggt caggaccgcc agggccgccg cccggaggaa
     5401 gaaaggagtc ctaggttcgg atgatcgcac ggtgcctccc gagagatcgg aatcgagggt
     5461 acgatgaaag taatacaaag taacactgtc gtcaagcgca tccggccgcc gacggccgaa
     5521 tcggagctca gctcgcgggc ccgatgtcca gctccagccc gtcgaaagcc ggccgcaggt
     5581 cgaacccggg ggaggacagc cccgagcaga ttgcttccaa ttcttcgtgg gagtggtcgt
     5641 ggcagatatg ggtgatccag gcctcccggg cccccaggga acgggccgcg tcggcggcct
     5701 cggaaatcga gaagtgggtg gggtggggcc gttcccggag ggctccgagg acgaggattt
     5761 caacgccctt caggatcggg cggctggatt cggggatacc ggagcagtcg gtcaggtagg
     5821 cgaagttccc gagccggaag cccaggatcg ggaggatccc gtgcagcacg ggcacgggca
     5881 ggacccggat cccgccgatc cggacgccct ccggggggag ctccggcagc cggatccggg
     5941 gcaccccgcc gcccctctgg ggagggtcga aggcgtagga gaaacggact tccagctccg
     6001 cccgggcctc ccggttcgcc cagacgggaa gggggccttc cctcgtgagg gggcgcacgt
     6061 cgtccaggcc gtggatgtgg tccgcgtggg catgggtcac caggaccgcg tccagccggt
     6121 cgatcccggc gcggagggcc tggaggcgga actcgggtcc gggatcgacc aggatccgtt
     6181 ccccgccgtc cccgcgcatg aggagggagg cccggtaccg ggagtcccgg ggatcctcgg
     6241 agcggcagac gggacagggg caacccacga cggggacgcc gtggctcgta ccggtcccca
     6301 ggaagacggc cctcacgcgg gatcgcgctt gatgtccacg ccccgttcgg tcaccgtccg
     6361 gccccgggtc cggaactgcg cggcggcctc ccgtatggag ctggtggtat ccgcgctctg
     6421 gcgggaaagg tcggaaagct ccgtggcgga acggtcgatc tcggagatgc cgagggtcat
     6481 ctcctgggcg gcgctgcggt tttccgcgga gagccggcgg acgtcctcca cggtgcggcc
     6541 gatcacggcc gtcccggact cgaggccccc ggacgccgtg gacagggcct ggctggtgcc
     6601 ggccaggccc tccagggcgg aaacgacctc ccggctccgg gacgccgcgt cctggagggc
     6661 gtcggaggtg gaggccagct cgcccatcag ctcctccagg ccgtcgatga cggacccgat
     6721 ggccccgttc gcggtttccg tggactccgc ggcgttccgg atcgcctccg cggtctcctc
     6781 gatcgtccgg cttatgccct gggcgttttc cgcggtactc tcggagagct tgcggatctc
     6841 ctcggccacc accgcgaagc cccggcccga ctcgcccgcg tgggccgcct cgatggcggc
     6901 gttcatggcc aggaggcccg tctgctcggc tatgccgttg atgatgtcca ccagttccag
     6961 ggtgcgccgg gcgctggcct ccatccgctc catggccgag gccgcggctt ccacccgttc
     7021 ccgtccgtcc gcccccaggc cggaaacccg cagggccgtc tccttctgcc gggatgcccg
     7081 ttccgccagg gtccggagct ccgtgccgat ctcgccgatc agcccccggg tgccctccac
     7141 ggcgttctcc aggtcccgga tggcggacag cacggtctgg gccgccgagg agatctcctg
     7201 ccgggccttc tcggtttccc ccgccgcgac gtccagggcc tcggtctggt gcttcatgga
     7261 ggctatgccc gcggacatct gggtgatggt ggccgcggtc tccgaggagt gggccgccag
     7321 ggtttccgcc gcggaggcgc tcttctccgc cgccccctgg atgtcctcca cgatggcgga
     7381 gagccggtcc gtcaaggcct ggaccgcggc ctggatctgg cctacctcgc ttgcggaccg
     7441 cttgaccgcg gataccgaga ggtccccggt ggacaccgcc gtgagcacgt cccggagttc
     7501 ccggacctgg gcggacactc tgcgggtcac gaccaggatg atcaggatgc ccgacaggaa
     7561 ggccgcggcg gcgacggccc cgcccccggc caaagccgcg tacaggcggc ccatgcgctc
     7621 ctcgacggag gtgtagacca ggatcgtccc atcgatgccg acgttcttgg acagggggct
     7681 ggagctgatg aaccaggatc cggatcccgc ccggatctcg tcgtaccagg tggaggaggt
     7741 ttccgaggag gcgctgacgg cgtccacggt ctcgtgggcc gccgcggcgg ccaccacgcc
     7801 gtccccgctc tcccggccgg gtatgcgctc gggcgttccg cctacgtccc gggtgaccgg
     7861 gaaatcccgg gacagggcga tgaggctgcc ccccgcgtcc aggagggcgg cgtccgcacc
     7921 ggatccctgg gcggcctcgg tgatcagggt cttcagggcg tccaggggca cgtccgcccc
     7981 gaggacgccg accacggctc ccgaggaatc cttcacggga acgtagggag taatgaccag
     8041 gcctttcgag acgaaatcca cgtagatctc cgtccaaccg ggctcgccgg attccgccgc
     8101 ggccttgtac cagccccggg tggtcggagc gaaatcggcg gcctcgcgga aggcgcccac
     8161 ggagccgtcc tggccgaggt cgtagaagtt cagcttgccc ccggtggtct cgtcccggat
     8221 gcagaccagc ccgcccccgt ccgcgttccg gctcaccagg accgtccgat cggaacggtc
     8281 cccgtagtac agggtggacg cggcgggagt gatgccggcg aactgataga ggagggtccg
     8341 gagggacgcg gtatccctca agtccaggtt cccggactcc acggccgcct ccagtccgga
     8401 cagtatggac cgggccgcgg tgaagtaccc ggcgagccgg gcgtcgatct ctcccgcggt
     8461 cttccaggtc agggagatgg acgattcccg cacgagtttc cggccggcct ggacggagct
     8521 caggacgacg gtacccacgg ccaggaggaa ggacagcacc gtcggaacca tgatcaggac
     8581 gaggaggggg accgtttttc catggttcga ttttgccaaa acctgctcct tatgcgaggt
     8641 attccgcggt tgggccggaa tataggaaca ttctaatccc cgggcgccgg cttgtcacgg
     8701 gcccgccgag gacggcaccg gcagtgccgg gggatcgaag tccggcaggg cggggatccg
     8761 gaccaggtgg gcgaattcgg agggataccg ccgcaggacc gcctccaggc tggtctcctc
     8821 ggcgctttcg aagacggcta cccggaactc cccgccggcg tcgaagtagg ggaacagcag
     8881 ggcttcgtgg aagtactgcc ggagcgtgac gctctcgccc atcctgcggt tgaaggaggc
     8941 gatgtagaag ttccccggtt cccggatggc cagcacgtag agcaggggac gcagaccgtc
     9001 cacccggtac ccgccgatct cgaaattggc ggggtagggc tcgtaggtct ggctcccgtt
     9061 ggcgggctcc gaactgcccc ggtccaccag ggcgaaggcg ggtacgcgga cgtcgttgtc
     9121 cgtgacgcgg ttgcggcggc tggggtacag ggcgtcggcc accgccttgc ccaggttcca
     9181 ggcccagtcc agcccgaaga agggatcccg cgccttctcg tagggctggg tgaaggagga
     9241 gcctcggaga tcctcgtggc gttctttgag ggcttccacg gagagccagg tacctgtgac
     9301 gggcttgagc atcccgtcca cgacccattt cgcgaatccg gaacagttga gcccgggatc
     9361 cccggcgggc acgcccccgg cgatgagcac cggcctgccc gtctcgtcca gggcgccgtc
     9421 gtcgccgtat cggagacctc ccagccgttc ccgcacggcc tgggccaggg atcgtagatt
     9481 cccgtagagc cccggcaccg gggacaggag ctcccagtcc acggagtccc gggtccatcc
     9541 cacgatgtcc cggagggggg aggtcaggag tttccgaaag ggcacgggca ggacgacgtc
     9601 gtggcgtacc accccgcggt agatgaccag gtccagcttg gtccggtccc cgaagggata
     9661 gagcctcaga aaggtgttcg ggtcgttccg gaggaagacc ttggcctgca cgaaccggcc
     9721 ggtcgagagg tcccgcttga tgatccacgc accctggccg tagagcacct cccggtccgg
     9781 gaccacggcg atgtagaagg cccggggacc ctggatcgtc tcgagtctcc agcggcccga
     9841 ggcgttttcc tcggtacggg tccggaatcc cagggcgcgt tcccggggag cctcgatgag
     9901 ctccgccatc cagcggaccc gcaggtcgtc gctctccggg atcgattcca ggggcaggtc
     9961 ccctcccagg ggaggggcga cctgggcgga caggggcagg gccgagacaa aagccaggag
    10021 caggaatccc attcgggcgc gcacggtcat ggaacctcgc ggcgattcag gatggcgtct
    10081 ccggcggcag gacggggggc gatccgcgtc gagggccggg cgtacatata taccatcgga
    10141 aagattccac gggaaatcga gggtgcccta cagacccgcc ggggaggctc cgatactgga
    10201 aggggctgtc cgaaacggtc ggaagacggt gaaggcgccc ttctcgcatc ccattcgcaa
    10261 ggacacctat gcgctcccag ctcgtgcgaa cggccctggc atccctggcc gcggcggcgg
    10321 ccgcggccgc gatcttcgcg gaaggcggaa cctccctggg cgaccccgcc cggcccggcc
    10381 tctcgggcgt ccccggtacg gcccggtccg agccgtaccg gctcttcccc cgggcctacc
    10441 ggccggaccc tgctccggcg gtctccgcag gccccgcgga tcaccacgtg cccgacgagg
    10501 cggaatacgc ggcccgcgag gccgccgccc ggtcccaggg acagcgggtg cccgtcctgg
    10561 ccaactccga catcctgtcg ttctacggcc accccacctc gcggcgcatg ggcatcctcg
    10621 gggaatatcc caaggaccag ctcacgagcc ttctgtccgg ctacgcccag ctcttcgacg
    10681 cggccaacgg agaccgggat gtgattcctg ccttccacct gatctacgga acctgctggc
    10741 ccgagggcga gatcggctac ctttcctcca agaccgtgga agaatacgtc cgcttcgccg
    10801 cggaacgggg catgctggtc ttcctggacc atcagatcgg gaagtacacg gtggaggagt
    10861 cggtacggag gctcctgccc ttcctccggt atccgaacgt gcacctggcc ctggatcccg
    10921 agtggcgcac cctttccccc atgaagga
//
LOCUS       DAOTEI010000118        10880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4270_length_10880_cov_5.334411, whole genome shotgun sequence.
ACCESSION   DAOTEI010000118 DAOTEI010000000
VERSION     DAOTEI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_10880_cov_5.334411"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="P5313_11430"
     CDS             <1..681
                     /locus_tag="P5313_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY81018.1"
                     /translation="KMLCLTSPANPTGCVESAENLKALADIARRHDLVVLADEIYSRI
                     YYGERNYAPSFYSVPGMPERTVIINGFSKTYAMDGWRLGWTVASEEMTRAILKVRQYT
                     TVCVNTFIQYGAAEALSADQSCVDQMVGEFSKRRTVMLDGLAKIPGVKVAEPKGAFYV
                     FPDIRSFGKSSAQVADYLLNEHHIACVAGSVFGPSGEGHLRLAYSCSTEECRKGVDRL
                     RTALSALK"
     gene            complement(729..1628)
                     /locus_tag="P5313_11435"
     CDS             complement(729..1628)
                     /locus_tag="P5313_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY81019.1"
                     /translation="MEFYGAVEAGGTKIVAAIGKAGEEPRALERIPTTDPGETLDRII
                     GFFQARVSEGLPVRALGVGCFGPVDLDPASPQWGFITSTPKLRWQGTDVAGTLGRALG
                     VPVGFDTDVNAAALAEYRLGAARGLDPAVYLTVGTGIGGGAVVNGRLLHGAAHPEMGH
                     MLVSRRGDDAFPGSCPFHGSCWEGLASGPAVEARWGRRGQDLPLDHPAWDLEAWYLAQ
                     GIVSLQMVLSPRAVILGGGVSEVPGLIDRVRSAAASLCAGYLDRPRTPTEWADVIRAP
                     GLKIPGLDGAFLLAEDAAGSRIA"
     gene            complement(1659..2114)
                     /locus_tag="P5313_11440"
     CDS             complement(1659..2114)
                     /locus_tag="P5313_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81020.1"
                     /translation="MKAKYFTGIFLTLAVSAAIVLMSGGTLPTFVDVPTYLLLILVPA
                     TLAFASWPVREIGRAFSAPFDPAAARNDLEKSRLFFQDLRKWLYTAAGLGTMLGLVSI
                     LVFADGEHLDRLGRNLGVMLLCVTNAFLLALMVPLPLESIVRRRLAELA"
     gene            complement(2111..2530)
                     /locus_tag="P5313_11445"
     CDS             complement(2111..2530)
                     /locus_tag="P5313_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81021.1"
                     /translation="MENGRRIDPDILLRRHFSREIEEERNLPTSRVQHATTDVEQDGA
                     SRWNDRPPSRAPRRSAWFQEAAAAAVLAACVGAAFLAVPGESSTAAPEVLGAKAVIVG
                     RLIETKLVWAVSEFADMRGPEPQGFGIPGRFREKERK"
     gene            complement(2517..3053)
                     /locus_tag="P5313_11450"
     CDS             complement(2517..3053)
                     /locus_tag="P5313_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY81022.1"
                     /translation="MRDDAADSRFLAHLDFRELCREWRPRAAAYCRVFRGLSDQDRED
                     VVGEALARAWAARDRFDPARPFAPWFLTIVRRAALDALARPGRRISPECVDDVPSPVP
                     GAEEAAVRDSEAEFVRRFVAALDPRDRELASLVYGQDLCLADAARTTGIPVGTVKWRL
                     HEIRIALRREWERENGER"
     gene            complement(3145..4863)
                     /locus_tag="P5313_11455"
     CDS             complement(3145..4863)
                     /locus_tag="P5313_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRY81023.1"
                     /translation="MSDIETASAPSNFIRDFVADDLATGRYDRVLTRFPPEPNGWLHI
                     GHAKAFSIDFGIAAEFGGKCNLRFDDTNPEKEDMSYVEAIKRDVKWLGYDWEDREFYA
                     SDYYEHLYEQAVKIIRKGKAYVDDLSEEEIKEYRGTNVPDKNNITHTPPGRDSPYRNR
                     TVEENLDLFARMRAGEFPDGSKTLRAKIDMAHPNLLMRDPVMYRIRREPHYRTGTAWC
                     IYPMYDFQHPLSDALEGVTHSLCSLEYEIHRPLYDWFIREAEAFPSRQIEFARLNLTH
                     TVLSKRWLLRLVQEGFVSGWDDPRMPTLAGLRRRGFTPESIRDFMNRIGVAKTDSFVD
                     VQLLEYCLREDLNRRARRVMAVLRPLKLVIENWPDGKVEELEAVNNPEDPSAGTRKVP
                     FSGTLYIERDDFMEVPPPKYFRLFPGNSVRLRYGYIVTCTGFDKDPATGEVTAVRCTY
                     DPETKGGNAPDNRKVKGTIHWVSAESAVPLEARLYDRLFPNERPMDVPPGVDWVGTMN
                     RDSLETVAGALGEPCLAECKPGDRYQFERLGYFTCDPDTNSGRRVFNRTVSLRDTWAK
                     IAQKGG"
     gene            complement(4934..5713)
                     /locus_tag="P5313_11460"
     CDS             complement(4934..5713)
                     /locus_tag="P5313_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY81024.1"
                     /translation="MKNILKEKLAAGARPVGTFFELGSESVVEALGKTGLDFLIVDNE
                     HGPFEAERTADFVRAAELARIAPLARVREISRPAVLKLLDVGVQGIIVPDVHTPDQVR
                     ALVDYAKFSPVGRRGFCPTRKDGWGFDYPATEDLRTNMAYCNAETLLIPQCETVGCLE
                     NIEAVAATEGVDGIFIGPFDLSISMGIPGQFEHPDFLRALGRIRDACRAAGKFSIYFT
                     VREDKVAEGFRVGFDVMTYSLDARILIQAYRDALDRIRKDI"
     gene            complement(5828..6286)
                     /locus_tag="P5313_11465"
     CDS             complement(5828..6286)
                     /locus_tag="P5313_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015268557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY81025.1"
                     /translation="MKTVRDILKSKGGEVVTIGPDSTALAALTLMAEKNIGAIMVVDS
                     KGDTVGIFSERDFARKIIVKGRECESTPVKDIMTKNITYAEPDTTIEACMNLMTDGRF
                     RHLPVKDKGKLAGVISIGDVVKALIKEQGKIIAEQAFEIGQNERKSGGVI"
     gene            complement(6353..7816)
                     /locus_tag="P5313_11470"
     CDS             complement(6353..7816)
                     /locus_tag="P5313_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY81026.1"
                     /translation="MTRLGLGLKLAYGAGDFGRACYNTLRMFFYAIFLTDVVGLDPLL
                     ASGAALVALVWDAVNDPLVGLLSDRVRTRWGRRRPFFLWFSVPFGLSFLVLWWAPPWS
                     SQAALAIHVTAAFMISDTFQTLVSVPYLALGPELAPGYDQRTSLMSWRMFFNLAASLV
                     AAGAGPELIDAALGAGLSLRSSYLVMGAVFGGISTVPFLLLFAVAREVPAAPPARGPG
                     FLGTLRILATNKPFVLAGSVYVLNWICFDLLALMLPYFLLYWLGGGDLLARVSLFGVR
                     LAPETAAFGILLATAILTLPFWNSLARHTSKRSAYLAGMGLWAAVQAVIGLLPRDRLS
                     SSLWVAALAGLFTSCAHILPESMFPEAIDWDELRSGERREGLYYGAVTFIRKLATAMA
                     TFLALQVLGRSGYAAPPEGALVFEQSASTLSAIRILTGPGVILVLVPVMLLAARIPVD
                     RRRQGRIRSLLDRRRALRSSRSGNIVKKKYRELADGG"
     gene            complement(7813..8943)
                     /locus_tag="P5313_11475"
     CDS             complement(7813..8943)
                     /locus_tag="P5313_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81027.1"
                     /translation="MVRIETVAPGDRNAVRRFIRVPFDFLAGEENWVPPLAGELRVLL
                     RRRHPFFEHSDAAFFLASEHGRDLGRIAVLDHRGYNGHHGTRTAFFCHFDVRDDGAAA
                     RALLDAASEWAAIRGLDRLIGPKGFLRSAGQGVLVDGFDRFPATGIPWNPPYYAGLLE
                     ASGFSKVTDFLSGYLDRNRPGDGRLYRVAEIARKRGAFEILAFRRRGEIRRWLPALKI
                     LQHEAFADNPNYVPSTDGEFALMSESMIFAADPSVVRFILRDGELAGFMGAFPNLGRG
                     IRRAGGELLPLGWYHILRERSRSRILDLNGLGILPKFRKMGGDAILFAELERLIRSSR
                     YESVEFIQVDERNFLSRSGIEHFGVDWAKRHRMYGKDLGGRR"
     gene            complement(8968..>10880)
                     /locus_tag="P5313_11480"
     CDS             complement(8968..>10880)
                     /locus_tag="P5313_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY81028.1"
                     /translation="WIRITLVNMLDDPVVQGILGNYHDITERKHAEQVNEFERRFAER
                     LLESMPGIFYLYTYPELRLVRWNRDHESLLGFEAREMKNRSIFEWHVPGADEAIRAAV
                     DAAMEKGLNQIEAPLIAKDGRRIPFLMTGVPFESEGRRYLMGIGIDLTDYKRIETELQ
                     ESRSRLSALFGAMTEMVVLHDLVFDEHGRPVDYRIIDCNEAYTRVTGIPKEEAVGRLG
                     SEVYGSDPAPYLKEFSDVGITGIPYHYSTYYAPMQKHFSISVVSPGTNRFATITTDIT
                     PLKEAETKLQLQVEEYEVLNEELRSSTEELKRSHELLTQCTEELDRYFSSSLDLLCIA
                     DTDGYFHRLNPEWENVLGYRIEDMEGRRFLDFVHPEDLDRTLKAVSSLDSQHSILNFE
                     NRYRCKDGSYRWIEWRSHPQGRLIYAAARDVTERKRTEEELKRRVEEKETLLKEVHHR
                     LKNNFASIEALVSMQAAEVQSPEARSALGDTLGRIAGMRILYEKLLATERYEESSVRE
                     YIESLVESMEDALSVHEGISLLPRVEDFQLPTKKLFPLGIIVNELVTNALKYAFEGRE
                     AGTVAIRLERIGPSATLEVRDDGKGLPEGFRIEDSRGFGITLVRMLAEQLGGTFSMES
                     AGGTRAELRFPV"
BASE COUNT         1903 a         3573 c         3549 g         1855 t
ORIGIN      
        1 aagatgctct gcctgacctc tccggccaac cccacgggct gcgtggagag cgccgaaaac
       61 ctcaaggccc tggccgacat cgcccgcagg cacgacctgg tggtcctggc ggacgagatc
      121 tactcccgca tctactacgg ggaaaggaat tacgcgccca gcttctattc cgtgccgggc
      181 atgccggagc gtacggtcat catcaacggc ttttccaaga cctacgccat ggacggttgg
      241 cgcctgggat ggaccgtcgc ctccgaggag atgacccggg ccatcctcaa ggtccggcag
      301 tacactacgg tctgcgtgaa caccttcatc cagtacggcg cggcggaggc cctgtccgcg
      361 gaccagtcct gcgtggatca gatggtcgga gaattctcca aacggcgaac cgtgatgttg
      421 gacggcctgg ccaagatccc gggagtgaag gtcgcggagc cgaagggcgc gttttacgtc
      481 ttcccggaca tccggtcctt cggaaaatcc tccgcccagg tcgcggacta cctcttgaac
      541 gagcaccaca tcgcctgcgt ggccggaagc gtcttcggtc cctccgggga aggacatctg
      601 cgcctcgcct actcctgctc caccgaggaa tgtcgaaagg gcgtggaccg actccggaca
      661 gcgctgtcgg cgttgaagta gttcaggccg ggccgtggct ggcgcatcag cccggccctt
      721 tcactaggct acgcaatcct cgagcccgcg gcgtcctcgg ccagcaggaa ggcgccgtcc
      781 agcccgggga tcttgaggcc cggggcccgg atcacgtccg cccattccgt cggagtccgg
      841 ggcctgtcca ggtagcccgc gcacaaggag gcggccgcgg accgcacccg gtcgatcagc
      901 ccgggaacct cggacacccc tccgcccagg atcacggccc ggggggatag taccatctgc
      961 agggatacga ttccctgggc caggtaccag gcctccaggt cccaggcggg gtgatcgaga
     1021 gggaggtcct gtccccgccg cccccatcgg gcctccacgg ccggcccgga agccaaccct
     1081 tcccagcagg aaccgtggaa ggggcaggag cccgggaacg cgtcgtcccc gcggcgggac
     1141 acgagcatgt gccccatctc cgggtgcgcg gccccgtgga ggagccggcc gttcacgacc
     1201 gcccctcccc cgatcccggt gcccacggtc aggtagaccg cgggatccag gccccgggcc
     1261 gcgccgagcc ggtactccgc cagggccgcg gcgttcacgt ccgtgtcgaa ccccacggga
     1321 acccccaggg ctcgccccag ggtgcccgcc acgtccgtcc cctgccaccg gagcttgggg
     1381 gtcgaggtga tgaagcccca ctggggggag gccgggtcga gatccacggg accgaagcag
     1441 cccacgccga gggcgcggac cgggagtcct tcggaaaccc gggcctggaa aaatccgatg
     1501 atccggtcca gggtctcccc gggatccgtg gtcggaatgc gctccagggc ccggggttcc
     1561 tcccctgcct tcccgatcgc ggctacgatc ttggttcccc cggcctcgac ggcgccgtag
     1621 aattccatcc ggatgctcct tccgcgcccc cggaatccct atgcgagttc cgcgagccgc
     1681 cggcggacga tcgactccag cggaagggga accatcaagg ccaacaggaa cgcgttggtc
     1741 acgcagagta gcatgactcc cagattcctg cccagccggt cgaggtgttc gccgtccgcg
     1801 aagaccagga tgctcaccaa acccagcatg gttccgagtc cggcggcggt gtacagccat
     1861 ttccgtaagt cctggaaaaa gagccgggat ttctccaggt cattccgggc ggcggcggga
     1921 tcgaagggcg cggaaaaggc ccggccgatt tcccggacag gccaggacgc gaaggcgagt
     1981 gtcgcgggca ccaggatcag cagcagatag gtgggcacgt ccacgaaggt cggaagggtg
     2041 cccccggaca tgaggacgat cgcggcggac accgcgagag taaggaaaat ccccgtgaaa
     2101 tacttcgctt tcattttcgc tccttttccc ggaatcgtcc tggaataccg aatccctgcg
     2161 gttccggacc ccgcatatcc gcgaattccg ataccgccca aaccagtttc gtttcgatca
     2221 atcgacccac gatcacggct tttgccccca ggacttccgg tgccgccgtc gaactctccc
     2281 ccggaacagc gaggaaggcg gcaccgacgc aggccgccag gaccgcggcc gccgccgcct
     2341 cctggaacca cgccgaacgt cggggtgccc tggatggggg ccgatcgttc caccgggaag
     2401 cgccgtcctg ctcgacatcg gtagtcgcat gctgcacgcg ggatgtcgga agatttctct
     2461 cttcctcgat ctcccgggaa aaatgccgcc tgagcaggat atccggatcg atccgcctac
     2521 cgttctccat tctccctctc ccattccctg cgcagggcga tccgaatttc gtggagcctc
     2581 cattttaccg taccgacggg aatccccgta gttcgtgccg cgtccgccag gcacaaatcc
     2641 tgtccgtaca ccaggctggc gagctcccgg tcccgcggat ccagggccgc cacgaagcgg
     2701 cggacgaatt cggcctccga atcccggacc gccgcctcct ccgcccccgg gaccggggat
     2761 gggacgtcgt cgacgcattc gggggagatg cggcgtcccg gacgggccag ggcgtccagg
     2821 gcggcccgcc tcacgatcgt gaggaaccag ggtgcgaagg gacgggccgg atcgaaccgg
     2881 tcccgggcgg cccaggcgcg ggcaagggcc tcccccacga catcctcccg gtcctggtcc
     2941 gacagccccc ggaagacccg gcaatacgcg gccgcccggg ggcgccactc gcggcagagt
     3001 tcccggaaat cgaggtgagc caagaacctg gagtccgccg catcgtcccg catcgtcacc
     3061 ctgcagtacg cggatatgcc gctgtggttg ggaaaaaaaa cgagggtctc gtaggacccc
     3121 cgctaggttc tacctccgag ccgctcaccc gcccttctgc gcgatcttgg cccaggtgtc
     3181 ccggagggat acggtccggt tgaacacccg ccggccggag ttcgtatccg gatcgcaggt
     3241 gaagtagccc agacgctcga actggtaccg gtccccgggc ttgcattccg ccaggcaggg
     3301 ttccccgagg gcgccggcta ccgtctccag ggaatcccgg ttcatggtgc ccacccagtc
     3361 caccccgggc ggcacgtcca tgggacgctc gttcgggaag aggcggtcgt agagcctcgc
     3421 ctccaggggc accgcggatt ccgcggatac ccagtggatg gtccccttca ccttacggtt
     3481 gtccggcgcg ttcccgccct tggtctccgg gtcatacgtg cagcgcaccg cggtgacctc
     3541 ccccgtggcg ggatccttgt cgaatcccgt gcaggtgacg atgtagccgt agcgcaggcg
     3601 cacagagttt ccggggaaga ggcggaaata cttgggcggc gggacttcca tgaaatcgtc
     3661 ccgctcgatg tagagggtcc cggagaaggg taccttccgg gtgccggcgg agggatcctc
     3721 ggggttgttg accgcctcca gctcctcgac cttcccgtcg ggccagtttt cgatgaccag
     3781 cttgagggga cggagcacgg ccatgaccct gcgggcccgc cggttcaggt cttcccgaag
     3841 gcagtactcc aacagctgga cgtccacgaa ggagtccgtc ttggccaccc cgatccggtt
     3901 catgaagtcc cggatggact cgggggtgaa gccccggcgg cgcaggcccg ccaacgtggg
     3961 catgcgggga tcgtcccagc cggatacgaa gccttcctgc acaagccgta ggagccagcg
     4021 cttggacagc accgtgtggg tcaggttgag ccgggcgaac tcgatctgcc ggctggggaa
     4081 ggcctcggcc tcccggatga accagtcgta caggggccgg tggatctcgt actccaggga
     4141 gcacagggaa tgggtgaccc cctccagggc gtccgagagg ggatgctgga agtcgtacat
     4201 cgggtagatg caccaggccg tgccggtccg gtaatggggc tcccttcgga tccggtacat
     4261 gaccggatcc cgcatgagga ggttcgggtg ggccatgtcg atcttggccc gcagggtctt
     4321 ggagccgtcc gggaactccc cggcccgcat ccgggcgaag aggtccaggt tctcctcgac
     4381 cgtccggttc cggtaggggc tgtcccggcc ggggggggta tgggtgatgt tgttcttgtc
     4441 cgggacgttg gtgccccggt attccttgat ctcctcctcc gaaaggtcgt ccacgtaggc
     4501 cttgcccttc cggatgatct tcaccgcctg ctcgtagaga tgctcgtaat aatccgaggc
     4561 gtagaactcc cggtcctccc agtcgtagcc cagccacttc acgtcccgct tgatcgcctc
     4621 gacgtagctc atgtcctcct tctcggggtt cgtgtcgtcg aagcggaggt tgcacttgcc
     4681 gccgaactcc gcggcgattc cgaagtcgat ggagaaggcc ttggcgtggc cgatgtggag
     4741 ccaaccgttc ggctccgggg ggaagcgggt caggacccga tcgtagcggc ccgtcgccag
     4801 gtcgtcggct acgaagtcgc ggatgaagtt ggagggcgcg gaggcggttt cgatatcgct
     4861 catgggaact ccttaccgcg cttcaggggc gcggcaggta gactatgccc cccgaggggg
     4921 ggacgggtca agatcagata tctttccgga tgcgatccag cgcgtcccgg taggcctgga
     4981 tgaggatccg ggcgtccagg ctgtaggtca tgacgtcgaa tccaacacgg aaaccctccg
     5041 ccaccttgtc ctcccggacg gtgaagtaga tggaaaactt tcccgcggcc cggcacgcgt
     5101 cccggatccg tcccagggca cgaaggaagt cggggtgctc gaactgcccg gggatgccca
     5161 tcgatatgga aaggtcgaag gggccgatga agattccgtc caccccctcc gtcgccgcga
     5221 cggcctcgat gttctccagg cagccgacgg tctcgcactg ggggatgagg agggtctcgg
     5281 cgttgcagta cgccatgttc gtgcggagat cctcggtggc ggggtaatcg aagccccaac
     5341 cgtccttccg ggtcgggcag aaaccccgcc ttcccaccgg cgagaacttc gcatagtcca
     5401 ccagggccct cacctgatcc ggggtgtgga cgtcggggac gatgatcccc tggacaccca
     5461 cgtccagaag cttcaggacc gcgggccggc tgatctcccg gacccgggcc aggggggcta
     5521 tgcgggccag ctccgcggca cggacgaaat ccgcggtgcg ttccgcctcg aagggaccgt
     5581 gctcgttgtc gacgatcagg aaatcgaggc ccgtttttcc cagggcctcg accacggact
     5641 ccgagccgag ctcgaaaaag gtcccgacgg ggcgcgcacc ggccgcgagt ttttccttga
     5701 gtatgttctt catgaaatcg ccttaatcga tgtattcctt ggtagtgcaa tttctcggaa
     5761 aagctctcgc ttttccaaga aattgcttaa cgtagggatg acaagctaga agcttgtcat
     5821 ccctacgtta gatgactccg ccggacttcc tctcgttctg gccgatctcg aaggcctgtt
     5881 cggcgatgat cttgccctgt tccttgatca gcgccttcac gacgtccccg atggagatga
     5941 cccccgcgag cttgcccttg tccttcacgg gaaggtggcg gaaccgtccg tcggtcatga
     6001 ggttcatgca cgcctcgatg gtggtgtcgg gttcggcgta cgtgatgttc ttggtcatga
     6061 tgtccttgac cggagtggat tcgcattcgc ggcccttcac gatgatcttc cgggcaaaat
     6121 cccgttccga gaagatgccc accgtgtcgc ccttggagtc caccaccatg atggcaccga
     6181 tgttcttctc cgccatcagg gtaagagccg ccagagccgt ggaatccggc ccgatggtca
     6241 cgacttcccc gcccttggat ttcaggatgt cccgtacggt cttcatggct ctcccctcct
     6301 tgtcgatccg ctgcttccta agttgatggt atacacctca ataatagaat ctctaaccgc
     6361 cgtcggcaag ctccctatat ttttttttca ctatgtttcc ggacctgctg gatcggagcg
     6421 cacgccgccg atccagcagg gatcggatcc gtccctgcct ccgccggtcc acgggaatcc
     6481 ttgcggccag gagcatgacg ggcaccagga ccaggatgac cccgggaccg gtcaggatcc
     6541 ggatcgcgga cagggtggag gcggactgct cgaagaccaa ggccccctcc gggggcgccg
     6601 catagcccga acgccccagg acctggaggg ccaggaaggt ggccatcgcc gtggccagct
     6661 tgcgtatgaa ggtgacggcc ccgtagtaca acccctcccg gcgctccccc gagcgaagct
     6721 cgtcccagtc tatcgcctcc gggaacatgg attccggcag gatgtgggcg caagaggtga
     6781 acagacccgc cagcgcggcc acccagaggc tcgaggagag ccggtcccgg ggcagcaggc
     6841 cgatgaccgc ctgcacggcc gcccagagcc ccatgcccgc gaggtaggcg gaccgcttcg
     6901 aggtgtgcct ggcgagggag ttccagaacg gcagagtcag gatggccgtc gcgaggagga
     6961 tgccgaacgc cgcggtctcc ggggcgagcc ggactccgaa cagggagacc ctggccagca
     7021 ggtcgccgcc ccccagccag tatagcagga aatacgggag catgagggcc aggaggtcga
     7081 agcagatcca gttgagcacg tagaccgaac ccgccaggac gaagggcttg ttggtcgcca
     7141 ggatccgaag ggtgcccagg aagcccgggc ccctcgcggg cggagccgcg gggacctccc
     7201 gggccacggc gaagagaaga aggaagggca ccgtcgagat gcccccgaag acggctccca
     7261 tcacgagata ggaactccgg agcgagagcc ccgccccgag cgccgcgtcg atcagctcgg
     7321 gtcccgcgcc cgcggcgacc agggaggccg ccaggttgaa gaacatgcgc cagctcatga
     7381 gggaggtgcg ctggtcgtag cccggcgcca gctcgggacc cagggccagg tagggcacgg
     7441 agaccagggt ctggaaggtg tcggagatca tgaaggccgc cgtcacgtgg atcgccaggg
     7501 ccgcctggga gctccagggc ggggcccacc acaggaccag gaaggaaagc ccgaagggca
     7561 cggagaacca gaggaagaag ggtctcctcc gcccccaccg cgtccggacg cggtccgaaa
     7621 gcaggcccac caggggatcg ttcaccgcgt cccagaccag ggcgaccagg gccgcaccgg
     7681 aggcgagaag aggatccagg cccacgacgt cggtcaggaa gatggcgtag aagaacatcc
     7741 gcagggtgtt gtagcaggcg cgcccgaaat cccccgcccc gtaggccagc ttgagtccca
     7801 gaccgagacg cgtcaccggc ggcctcccag gtccttcccg tacatcctgt gccgcttggc
     7861 ccagtccacc ccgaagtgct cgataccgga ccgggacagg aagttcctct cgtccacctg
     7921 gatgaactcc acggactcgt agcgggagga tcggatcagc cgctccagct ccgcgaacag
     7981 gatggcgtcg cctcccatct tccggaactt cgggaggatc cccaggccgt tcaggtcgag
     8041 gatcctcgag cgggacctct ccctcaggat gtggtaccag cccaggggaa ggagctcccc
     8101 gcccgccctg cggattcctc ggccgaggtt gggaaaggct cccatgaagc cggccagctc
     8161 cccgtcccgg aggatgaagc ggaccaccga cggatccgcc gcgaagatca tggattcgga
     8221 catgagggcg aactccccgt ccgtggaggg cacgtagttc ggattgtccg cgaaggcctc
     8281 gtgctggagg atcttgagcg cgggcagcca ccgccggatc tcgccccggc ggcggaaggc
     8341 gagtatctcg aaggcgccgc gcttgcgggc gatctcggcc acccggtaga ggcgaccgtc
     8401 cccgggccgg ttccggtcca ggtatcccga caggaagtcc gtcaccttgg agaagccgct
     8461 ggcctcgagc agcccggcgt agtacggggg attccagggg atgcccgtgg cgggaaacct
     8521 gtcgaatccg tcgacgagga cgccctgccc ggcggagcgc aggaagccct tgggcccgat
     8581 caggcggtcg agtccccgga tggccgccca ctccgacgcg gcgtcaagga gggcgcgggc
     8641 ggcggccccg tcgtcccgga cgtcgaaatg gcagaagaac gccgtgcgcg tcccgtggtg
     8701 tccgttgtag ccccggtgat ccaggaccgc gatacggccg aggtcccggc cgtgctcgga
     8761 ggccaggaag aatgcggcgt cggagtgctc gaagaaggga tgccgccgcc ggaggaggac
     8821 ccggagctcc ccggccaggg gaggaaccca gttctcctcc ccggcgagga agtcgaaggg
     8881 gactcggatg aagcggcgga ccgcgtttcg gtctcccgga gccacggttt cgatgcgcac
     8941 catacctcct ggcgcaaggt tcatgtccta taccgggaac ctcagctccg cccgggtccc
     9001 gccggcggat tccatggaga aggttccgcc cagctgttcc gcgagcatgc ggaccagggt
     9061 gatgccgaat ccccggctgt cctcgatccg gaagccctcc gggaggccct tcccgtcgtc
     9121 ccggacctcc agggtcgcgg agggaccgat ccgttccagg cggatcgcga cggtccccgc
     9181 ctcccggccc tcgaacgcgt acttgagggc gttggtgacg agctcgttca cgatgatgcc
     9241 cagggggaag agcttcttcg tcgggagctg gaagtcctcc acccggggca ggagggaaat
     9301 cccctcatgg accgagagcg cgtcctccat ggattcgacg aggctttcta tgtattccct
     9361 cacggagctc tcctcgtatc gctccgtggc caggagtttt tcgtagagga tccgcattcc
     9421 cgcgatcctg ccgagggtgt cgcccagggc ggaacgggct tccgggcttt ggacctcggc
     9481 ggcctgcatg gacaccaggg cttcgatgga ggcgaagttg ttcttgagcc tgtggtgcac
     9541 ttccttgagg agggtctcct tttcctcgac cctccgcttg agctcctcct ccgtccgctt
     9601 ccgttccgta acgtcccggg ccgcggcgta gatcaggcgc ccctggggat gggatctcca
     9661 ctcgatccag cgataggagc cgtccttgca ccggtaccgg ttctcgaaat tcaggatgct
     9721 gtgctgggaa tcgagggagg agacggcctt gagggttctg tccagatcct cggggtggac
     9781 gaaatccagg aagcgccgtc cctccatatc ctcgatacga taccccagga cgttctccca
     9841 ctcgggattc agtcgatgga agtatccgtc cgtgtccgcg atgcacagga ggtccagact
     9901 gctggaaaaa tagcggtcca gttcctcggt gcactgggtc aacaattcgt gggagcgctt
     9961 caattcctcg gtggaggatc ggagctcctc gttgaggact tcgtactcct ccacctgaag
    10021 ctggagcttg gtctcggcct ccttcagggg cgtgatgtcc gtggtgatcg tggcgaaccg
    10081 gttcgtcccg ggggagacga cggagatcga gaagtgcttc tgcatggggg catagtaggt
    10141 cgaataatgg tacggtatcc ccgtgatacc gacgtccgag aactccttca aatagggcgc
    10201 cggatccgag ccgtacacct cgcttcccag acggccgacg gcctcctcct tggggatacc
    10261 cgtgacgcgc gtataggctt cgttgcaatc gatgatgcgg taatccacgg gacgaccgtg
    10321 ttcgtcgaag accaggtcat gcaggaccac catttcggtc atggccccga agagggcgga
    10381 aaggcgggac cggctttcct gaagttcggt ctcgatccgc ttgtaatcgg tcaggtcgat
    10441 tccgataccc atgaggtatc ggcgtccctc cgattcgaag gggactccgg tcatgaggaa
    10501 gggtatcctc cgcccgtcct tcgcgatcag cggggcttcg atctggttca agcccttctc
    10561 catggccgca tccacggccg cccggatcgc ctcatcagcg cccggcacgt gccactcgaa
    10621 gatggagcgg ttcttcattt cccgggcttc gaatcccaac aacgactcgt ggtcccggtt
    10681 ccatcgcacc agcctaagct cggggtaggt gtagaggtag aagatcccgg gcatgctttc
    10741 caggagccgc tcggcgaacc tccgctcgaa ttcgttcacc tgctccgcgt gctttcgttc
    10801 cgtgatgtcg tgatagttcc cgagaatgcc ttggaccacg ggatcgtcga gcatgttgac
    10861 cagggtgatc cggatccact
//
LOCUS       DAOTEI010000119        10829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4292_length_10829_cov_6.119547, whole genome shotgun sequence.
ACCESSION   DAOTEI010000119 DAOTEI010000000
VERSION     DAOTEI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4292_length_10829_cov_6.119547"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(489..941)
                     /locus_tag="P5313_11485"
     CDS             complement(489..941)
                     /locus_tag="P5313_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81029.1"
                     /translation="MRRQLSLTEQRQEEERTAGDFLAFFEGRQDLGAVIYQEWTAKDV
                     LGHVASWHLSFARNLLDAVNGVNPSPFKGTLSEVNERGVREMSVHPPGEILRMIREAQ
                     ATISAHIEDPRVTEIAYKKGSRNYSPIEHLEVVRRHIDGHLADLRRKG"
     gene            1240..2574
                     /locus_tag="P5313_11490"
     CDS             1240..2574
                     /locus_tag="P5313_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HRY81030.1"
                     /translation="MVRRRIRFRHAVLTAVLAGALAVPAAAGDVELEIRQTEWELRPG
                     LTTRAWTYGGTVPGRPIVVRPGERVRATVTNRLPVATNIHWHGLVLPNDQDGPALTIE
                     PGAVFRYDFLAGESGTYWYHPHRMPVLPQMDRGLYAPFIVLAPEDALYDGDHTFVLDD
                     WLLDSRGNRLEGTSRQEMERYGNVETVNGKTGDAVAPLRVRAGQIHKLRFINASTAAF
                     HELKVSGHRFRVTHLDGHPLQRPYETDRIDLAPGERVDAELFASRHEGERYRISSSRP
                     ELGLVIPIVYGPGSVPASASPFRPPAPEAPAAVPARPDQELVLSSGMGGMMGPGGMVW
                     TIDGRAFPRTRPIEAKVGVPLTLRIYNRDHMSMMMGHRMDHPIHVHGTRFRVLSVNGT
                     APDQPIWKDTVPVPLGGFVDISFVMKNPGDWMFHCHIIDHEDGGMMTFVRAR"
     gene            2718..5480
                     /locus_tag="P5313_11495"
     CDS             2718..5480
                     /locus_tag="P5313_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81031.1"
                     /translation="MKDAGPQRLYLRLVNALSFSSELYKLRRELNARIDAPATIAQGP
                     RSHPLGVSRWFKKRRISIAEAYIMVVSDLESKHSRARLRALRMLVEVSFHAKTLDLPL
                     NTARVQLGLIKEAIKHKGDRRRQLELLQDFSVSSHGQQSVIRKLLAELNVVELPETDS
                     RLKNLDAGWDDHVHDTATSGRKNPTQLLIDAFIKGISELTIAYGSIDSIDLMKEALDA
                     GRTLGIRVNLALEFSILIQGRRFHFLATLPRFKNGKDAARWFKQQSRDFRDILDGLRK
                     NQENRVEAVRALLKHFNAGGLEELNSGWPGDTLYVLPKLKFGELTESIPKSSLNRMHL
                     GEYLYSRYKPILFNRVMLTKVHREKARTALRRKLLSDWEYRIVDDRYARIREEYRKLN
                     PESLRKQYFTNPEIGDYQTIFTDLRKMHSILAGAGCTLRVLHPLEYGYEAARRLLESS
                     RGIIDEVEIFNMQDSLGRDPEDNLRLARLVNELNVQSAAAGKTPFVPVCGSDSTGRTP
                     TVPGMGFIREDRITGTRRKAYAKRHIALPPLVSAMVEARGLPVSESEASEAPRILSLG
                     KMTGGRTNLIGDETDAEAAIIPPSRAWRYLNPWLVNLLYSAVGFLVAQRFVGTFYAFL
                     WLFITGFRNSVADLVAGRGSRLSAWSLKGVNFDNVARSLFWTGFSVPIMAFVKARFDA
                     LWPFAAEGLLFDAAKFFFIAFANGLYLAAHNTLRGFDRKVVRANFFRSVIAWPLATAF
                     APFGAALGIPSIVHSKIWSDVVAGFIEGGSKYVKTLGLRQRDIREILPQLMEGDKEER
                     FTALLDLLFLYRQEPRTETSLKALLVSEKPSEGETEDDAERSDYAFDEFRAAVMDENS
                     DDELAAFILSTYQPEMAADLVSLVSSTLPSFRDWVTSHARLFKRSIPAKEIDVPAAVR
                     AAQPLE"
     gene            5493..5990
                     /locus_tag="P5313_11500"
     CDS             5493..5990
                     /locus_tag="P5313_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY81032.1"
                     /translation="MATIPRPEDPNREWLVLHLTGMTAHIPFERAVKDFPVELAGKRI
                     PGLDHTAWGLLWHLAVCQEDILLYALNEGHVSPPYPSGLWPEEDGPAEAADWERTAMK
                     FNRDLEAVVRMVRDPGRDLYAPVRPGIDETLLQMAGLVIDHNSYHIGQFVDLRMLLGV
                     PVKDW"
     gene            6059..7219
                     /locus_tag="P5313_11505"
     CDS             6059..7219
                     /locus_tag="P5313_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HRY81033.1"
                     /translation="MDDGITRLFENHRAAEFLERPNRFVIRARDPETGEVLVCHCPNP
                     GSLAELALPGTPLILERRRTPGATAWTAAAALYRGGVVPLYSARANAVTRDLVIPRLF
                     PEASGIRPEFRIGGSRFDFYFEDPAGGRHLAEVKSCSLVERGVAMFPDAPSARASRHL
                     AELAALSREGWTCHVLFLIQHGYPRVFIPNLHTDPAFAAALGTLSPGLDLRAVSLETS
                     EDGRVRIVSDRVAIDLGHTDLADADRGSYLVILELAEPAEIETRSLGRIDFPAGWYVY
                     AGSARKGLSARVARHLRRSGKRLRWHVDYLARRARSARGLALASYDNLECELAEALTR
                     IGGRRIPDFGSSDCRCPGHLLRFASDPMKDRGYLDVLFYYRHERALEGNPRG"
     gene            complement(7235..8530)
                     /locus_tag="P5313_11510"
     CDS             complement(7235..8530)
                     /locus_tag="P5313_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY81034.1"
                     /translation="MSFFSARSIFPIIKKNVLFSFLSELAPIYPAYMILFRERGYDFL
                     ELSLLFVVWELSVVVLELPSGILADRWDKRKVLAVGMLLKALGFCAWLAWPSFTAAAV
                     GFALWGAQEAFCSGTRQALLYEALRDRGRETSYDKAAGISNAAATSAVALSLVIGGPL
                     FAGSPGLTLALSAAAAAGGALSALSLRTRRASDLSPRASAAGFPEGTGEGAGAGGERS
                     PGTGLAATLGSFRRALRDRRLALLLAAACLSIAAYGTLDEFDGLWALERYGVPLAWVG
                     VWGALRFGAEGVGGLAAGFLLKSGSAGRKGKIGPAVGAAGVAFAAAAFLPGRFGVPLY
                     LGGYFLMSAVNVAFETRLQEAARDETRAAFLSLSSLLMTLCALALAPLLGLAGDARGL
                     PGIFAVGGGIILAAALLMAAGRIFGRARRDRAGESEQAS"
     gene            complement(8728..9900)
                     /gene="gguB"
                     /locus_tag="P5313_11515"
     CDS             complement(8728..9900)
                     /gene="gguB"
                     /locus_tag="P5313_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY81035.1"
                     /translation="MGSIKDLLRNNVRQYGMLIALVFIMLFFQVATGGVLLVPMNVTN
                     LVLQNAYVFILAIGMTLCILTGGNIDLSVGSVVAFIGALSGTFIITLDMGVGASIALV
                     LAVGLVIGVWQGFWIAYARIPAFIVTLAGMLTFRGLTVFMLKGLTIGPFPESFQQISS
                     GFVPDLIGGEESLYNWTALAAGAALALLFVLSESIQRGYRRRNGYESLPLPLFAAKLA
                     LTVGVIALASYWLASYRGIPVIFIVIGTLVAAYSFLTTRTVPGRYLYAMGGNEKAAQL
                     SGIDTNRVLFLAYVNMGMLSAVAGIAVASRLNAASPLAGQNFELDAIAACYIGGASAS
                     GGIGTIVGAIIGALVMGVLNNGMSILGIGSDIQQFVKGMVLLLAVAFDVISKRRAR"
     gene            complement(9887..>10829)
                     /locus_tag="P5313_11520"
     CDS             complement(9887..>10829)
                     /locus_tag="P5313_11520"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY81036.1"
                     /translation="VEVVEVADSITILRDGATIETLETSSGDIDEDRIIRGMVGRDLV
                     DRFPRREPRIGETAFEIRDWNVYSPLSPDRRIVKDASLTLRKGEVVGLAGLMGAGRTE
                     LAMSLFGRSYGVRVSGSAFKDGRPVELRSVRRTIDQGVSYATEDRKTAGLNLIGDICE
                     NITIAGLGKVAERSVIDRDREAKVAEDFRSRLKIRSTSIRQKAGDLSGGNQQKVVLSK
                     WIFTMPDVLILDEPTRGIDVGAKYEIYSIINQLADEGKSVLFISSELPEILGICDRIY
                     VMNDGRIVGEFPREEATQEGIMKCIMQSVRENGNGKH"
BASE COUNT         1935 a         3615 c         3513 g         1766 t
ORIGIN      
        1 gtagagcgat aggttcgcaa tctataggtc aggggttcga atcccctatt ctccaaccga
       61 cgaaaaccgg ccgtcaggcc ggttttcatc attaatagaa taggggattc gaaccgaagg
      121 cgcgcggcgc ggaagcgccg gaaggcgccc caggatgggc gccgccagcg cccgaggcgg
      181 cccggagatc gaggacagga tgtccgagat cgtagggcga atccccaacg gacacgacgc
      241 gaaactgcgc attcgaaccg gagcgtcgtc ccgagcgcat accgagggct cgccgcaggg
      301 atgcggcgaa aaacgcgaag gcggcccgga agcccgaggc aggatgccgt cgtgcgcgat
      361 agcgtacacg tcaatcaact acctctcgac aaacgacgcg aagcggcgta tgccgcgggc
      421 tggagggcga agcccctccg gaagcggcgc attcagccgc gcgcggcagc gacgaaacgc
      481 cgcggctcct accccttcct ccgcaggtcc gccaggtgac cgtcgatgtg ccggcggacc
      541 acctccaggt gctcgatcgg agaataattg cgggaaccct tcttgtaggc tatctccgtg
      601 accctcgggt cctcgatatg ggcggagatg gtcgcctggg cttcccggat catccggagg
      661 atctcgcccg gagggtgcac ggacatttcc cgcacgcccc gctcgttcac ttccgagagg
      721 gttcccttga acggagaggg attgacgccg ttcaccgcgt ccaggaggtt ccgggcgaaa
      781 ctaaggtgcc aggaggcgac atgtcccagg acgtccttgg cggtccattc ctgatagatg
      841 accgcgccca ggtcctgccg cccttcgaaa aaggcgagaa aatcgccggc cgtccgctcc
      901 tcctcctgcc tttgctctgt cagggatagc tgacgtctca tggacacccc cgggtaagta
      961 tatcgccccg cccttgagaa gctccgcgta gctgataagg tcactgcagt gtgcgggcac
     1021 gggcggttat ccgtctacgg tccccttctt aatctagaca atacactgaa cggagagcgt
     1081 tttagaatca ctacgtagcc gcccgacatg gcgatcgtag acctcaccgc ccagcgggac
     1141 agccgggatg ggatttcggg aacgcgcaca cagggttttt attagtatat ttgtaatatt
     1201 ctcggtcctc cgatccggga aggggaggtg atcttgagaa tggtccgacg gcggattcgt
     1261 tttcggcacg cggttcttac agccgtgctg gccggggctc tcgccgtccc ggcggcggcc
     1321 ggggatgtgg aactggagat ccggcagacc gagtgggagc tgcggccggg gctgacgacc
     1381 cgggcctgga cctacggcgg aacggtgccg ggccggccca tcgtggtccg cccgggggag
     1441 cgggtccggg cgacggtgac caaccgcctt ccggtcgcga cgaacatcca ctggcacggc
     1501 ctcgtcctgc cgaacgacca ggacggcccc gccctcacga tagagcccgg ggccgtgttc
     1561 cggtacgact tcctagcggg ggagagcggg acctactggt accacccgca ccggatgccg
     1621 gtacttcccc agatggaccg cgggctctac gccccgttca tcgtactggc cccggaggac
     1681 gccctctacg acggggacca cacctttgtg ctggacgact ggctcctgga ttcccggggc
     1741 aaccggctcg aggggacctc ccggcaggag atggagcggt acgggaacgt ggagaccgtg
     1801 aacggaaaga ccggggatgc agtggcgccc ctgcgggtcc gggccggaca gatccacaag
     1861 ctccgcttca tcaacgcgtc gaccgcggcc ttccacgagc tcaaggtatc cggccatcgg
     1921 ttccgggtga cgcacctcga cggccatccc ctccaacgtc cctacgagac ggatcgcatc
     1981 gacctggccc cgggcgagcg ggtggacgcg gagttattcg cgtcgcggca cgagggggaa
     2041 cggtacagga tctcctcctc ccgcccggaa ctcggcttgg tcatcccgat cgtgtacgga
     2101 ccgggatccg tcccggcgtc cgcctccccc ttccgtcctc ccgccccgga ggctcccgcg
     2161 gccgttcccg cgcgcccgga ccaggagctc gtcctgtcct ccgggatggg cggcatgatg
     2221 ggccccgggg ggatggtgtg gaccatcgac ggccgggcct tcccccgaac ccggcccatc
     2281 gaggcgaagg tcggggttcc cctgaccctg aggatctaca accgggacca catgtccatg
     2341 atgatgggcc atcgcatgga ccatcccatc catgtccacg gcaccaggtt ccgggtgctg
     2401 tccgtcaacg ggactgcgcc ggatcagccg atctggaagg ataccgtgcc ggtccccctc
     2461 ggcgggttcg tcgatatttc cttcgtgatg aaaaaccccg gggactggat gttccattgc
     2521 cacatcatcg accacgagga cggagggatg atgaccttcg tgcgggcgcg ctaggccgaa
     2581 gggtaccgct ccgggcggct tgacgtcctg agaaccgagg tccatcgtta tgaccggacg
     2641 gccggtcgaa cgtcacccgg acgctccgca gaacggcccg ttatccatac cgcgtacttg
     2701 tggtagacta agataccatg aaggatgcgg gcccccagcg cctataccta aggctcgtca
     2761 acgccctgag tttttcctcg gagctgtata agcttagacg ggaactgaac gcccggatcg
     2821 atgcgcccgc aacgatcgcc cagggccccc gctcccatcc gttgggggta tcccgctggt
     2881 tcaagaaacg caggatatcg atcgcggaag cctacatcat ggtcgtgagc gacctggagt
     2941 ccaagcattc ccgggcaagg ctccgggctc tccggatgct ggtcgaggtg tcgttccacg
     3001 ccaagaccct cgaccttccg ctcaacacgg cgcgcgtcca gctggggctc atcaaggagg
     3061 cgatcaagca caagggcgac cggcgtcgcc agctcgaact cctccaggat ttctccgtat
     3121 ccagccacgg acagcagtcg gtgatccgga agctcctggc tgaactcaac gtcgtcgagc
     3181 ttccggagac cgattcgcgc ctcaagaacc tggacgcggg ttgggacgac catgtccacg
     3241 acacggcgac ctcgggccgc aagaacccga cccagctcct gatcgacgcc ttcatcaagg
     3301 ggatctccga gctgaccatc gcctatggat cgatcgattc catcgacctg atgaaggagg
     3361 ccctggacgc gggcaggact ctgggcatcc gggtcaacct tgccctcgag ttcagcatcc
     3421 tgatccaggg ccgccgcttc cacttcctcg cgaccctacc ccgcttcaag aacggcaagg
     3481 acgcggcccg atggttcaag cagcaatccc gcgatttccg ggacatcctg gacgggctca
     3541 ggaagaacca ggagaaccgg gtcgaggcgg tccgggccct tctcaagcac ttcaacgccg
     3601 gcggactcga ggagctcaac tcaggctggc ccggggatac tctgtacgta ctgcccaagc
     3661 tgaaattcgg cgaattgacc gagtcgatcc cgaagtcgag cctgaaccgc atgcatctgg
     3721 gagaatacct gtattcccgc tacaagccca tcctcttcaa ccgcgtcatg ctgacgaagg
     3781 tccatcggga gaaggcgcgg acggctttgc gcaggaagct cctctcggac tgggaataca
     3841 ggatcgtgga cgatcggtat gcccgcatcc gggaggaata ccggaagctc aaccccgaga
     3901 gcctccggaa acagtacttt accaaccccg agatcggcga ctaccagacg atcttcaccg
     3961 atctccggaa gatgcattcc atcctggccg gggcgggatg cacgctccgg gtcctgcatc
     4021 ccctggaata cgggtacgag gcggctcgga ggctcctgga atcgagccgc gggatcatcg
     4081 acgaggtcga gatcttcaac atgcaggatt ccctgggccg cgacccggag gacaacctga
     4141 ggctggcccg gctggtgaac gaactcaacg tccagagcgc ggctgccggg aagaccccgt
     4201 tcgtaccggt ctgcggcagc gattccaccg gacggacccc gacggtgccc ggcatgggat
     4261 tcatccgcga ggaccggatc acgggaacgc gcaggaaggc gtacgccaag cggcacatcg
     4321 cgctgccgcc cctggtctcg gcgatggtcg aggcgcgcgg gcttcccgta tccgagtcgg
     4381 aggcgtccga ggctccgagg atcctgagcc tcgggaagat gacgggcggc cggaccaacc
     4441 tgatcggcga cgagactgac gccgaggcgg ccatcatccc cccctcccgc gcctggcgct
     4501 acctcaatcc gtggctcgtt aatttgctgt attccgcggt gggcttcctt gtggcccagc
     4561 gtttcgtggg caccttctac gccttcctgt ggctcttcat cacggggttc cggaactcgg
     4621 tcgccgacct cgtggcgggg cgcgggtccc ggctcagcgc ctggagcctc aagggcgtca
     4681 atttcgacaa tgtggcacgg tccctgttct ggaccgggtt ctccgtgccc atcatggcct
     4741 tcgtcaaggc gagattcgac gcgctctggc ccttcgcggc ggagggcctg ctcttcgacg
     4801 cggcgaagtt cttcttcata gccttcgcca acggcctgta tctggcggcc cacaataccc
     4861 tgcggggctt cgaccggaag gttgtccggg cgaatttctt ccgcagcgtg atcgcatggc
     4921 ccctggccac ggccttcgcc cccttcggag cggcgctggg catcccgagc atcgttcact
     4981 cgaagatctg gtcggacgtc gtggccggat tcatcgaggg cgggagcaag tacgtgaaga
     5041 ccctcggact ccgccaacgg gacatccggg agatcctgcc ccagttgatg gagggagaca
     5101 aggaggagcg cttcacggcc ctcctcgacc ttttgttcct gtaccgacag gaaccccgca
     5161 cggagacctc gctgaaggct ctcctcgtat ccgaaaagcc ttcggaaggc gaaaccgagg
     5221 atgacgcgga acggtccgat tatgcctttg acgagttcag ggccgcggtc atggacgaga
     5281 actcggacga cgaactggcc gccttcatcc tgtcgacgta ccaaccggaa atggccgcgg
     5341 acctcgtgag cctggtctcg tccaccctgc cctccttccg ggactgggtc acatcccacg
     5401 cgcggctctt caagagatcg atcccggcca aggaaatcga cgtcccggcc gccgtccgag
     5461 cggcccaacc tttggagtaa ggaggcggaa tcatggcgac gataccaagg cccgaggacc
     5521 cgaaccgtga atggctggtg ctccacctca cgggaatgac tgcccatatc cccttcgagc
     5581 gggccgtgaa ggatttcccc gtggagctcg ccggaaaacg gatacccggc ctggatcaca
     5641 cggcctgggg ccttctgtgg cacctggctg tctgccagga ggacatcctc ctctacgccc
     5701 tgaacgaggg gcacgtgagc cccccctacc cttcgggcct gtggcccgag gaggacggtc
     5761 ccgcggaggc cgcggactgg gagcggacgg ctatgaagtt caaccgggac ctggaggccg
     5821 tcgtccggat ggtccgggat cccgggcggg acctgtacgc cccggtccgg cccggtatcg
     5881 acgaaaccct cctccagatg gcgggcctcg tgatcgacca caactcctac cacatcggcc
     5941 agttcgtgga cctgcggatg ctcctggggg tgccggtcaa ggactggtag ggcccgcggt
     6001 ccggcgtcct caaccccgga ccttgccttc ggaccaaggg cggtcgatac tctcccccat
     6061 ggatgacggg atcacgaggc tcttcgagaa ccaccgggcg gcggagttcc tggagcggcc
     6121 gaaccgcttc gtcatccggg cccgggatcc ggagaccggg gaggtcctgg tctgccactg
     6181 ccccaatccc ggaagcctcg cggagctggc cctgccgggc acccccctga tcctggaacg
     6241 ccgccggacc cccggcgcga ctgcctggac cgccgccgcg gcgctgtacc ggggcggagt
     6301 cgtgcccctg tattccgccc gggcgaacgc cgtcacccgg gacctggtaa tcccccggct
     6361 cttcccggag gcgtccggga tccgccccga gttccggatc ggcggctccc ggttcgattt
     6421 ctatttcgag gatcccgccg gaggccgcca cctggcggaa gtgaaatcct gctccctggt
     6481 ggagcggggc gtcgcgatgt tccccgacgc ccccagcgcc cgggcgtcgc ggcatctcgc
     6541 ggagctggcc gccctgtccc gggagggctg gacctgccac gtcctcttcc tgatccagca
     6601 cggatatccc cgggtcttca ttcccaacct ccataccgac cccgccttcg ccgcggccct
     6661 gggaaccctg tcgccgggcc tggatctccg tgcggtcagc ctggaaacat ccgaggacgg
     6721 ccgggtccgg atcgtctccg accgtgtggc catcgacctg gggcacacgg acctggcgga
     6781 cgcggatcgg ggaagctacc tggtcatcct ggagctcgcc gaacccgcgg agatcgagac
     6841 caggagcctg ggccggatcg acttcccggc gggctggtac gtctacgcgg gttccgcgcg
     6901 gaaaggcctt tcggcccggg tagcccgcca cctgcggaga tcggggaaac gactccgctg
     6961 gcacgtggac tacctggccc ggcgggcccg gtccgcccgg ggcctggctc tggcgtccta
     7021 cgacaacctc gagtgcgagc tggccgaagc cctgacccgg atcggcggcc ggagaatccc
     7081 ggacttcggc tcctcggact gccgctgtcc cggccacctc ctccgcttcg cctcggaccc
     7141 gatgaaggac cggggatacc tggatgtcct cttctactac cgacacgagc gggctttgga
     7201 ggggaatccc cgagggtgag gcccgttccc ggtgtcacga agcctgctca gattctccgg
     7261 cccgatcccg ccttgccctg ccgaaaatcc tacccgccgc catcagcaag gcggccgcca
     7321 ggatgatccc cccgccgacg gcgaagatcc ccggaaggcc tcgggcgtcc ccggccagtc
     7381 cgagcagggg cgcaagggcc agggcgcaga gggtcatgag aagggacgag agagacagga
     7441 acgccgcccg cgtctcgtcc cgggctgcct cctgcagccg ggtctcgaag gccacgttga
     7501 ccgcggacat gaggaagtac cctcccagat agaggggaac cccgaaccgg cccggaagga
     7561 aggcggcggc cgcgaaggcg accccggctg cgcccacggc cgggccgatc ttgcccttcc
     7621 ttccggcgga tccggacttg agaaggaatc cggccgccaa tccgccgact ccctcggccc
     7681 cgaaccggag ggctccccag acccccaccc aggccagggg cacaccgtag cgctccaggg
     7741 cccaaaggcc gtcgaactcg tccagggtcc cgtaggcggc gatggacagg caggccgcag
     7801 ccaggaggag ggccagccgc cggtcccgga gggccctccg gaaggatccc agggtggccg
     7861 ccaagccggt ccccgggctt ctctccccgc cggcccccgc tccctctccg gtgccctccg
     7921 ggaaccccgc ggccgacgcc cggggcgaga ggtcggatgc cctccgggtc cggagcgaaa
     7981 gcgcggacaa ggcgcctccg gccgccgccg ccgcggacag ggccagcgtg aggccgggac
     8041 tcccggcgaa aaggggccct ccgatgacca gggacagagc tacggcggaa gtagccgcgg
     8101 cgttcgagat ccccgcggcc ttgtcgtagg aggtttccct gccgcgatcc cggagggcct
     8161 cgtagagcag ggcctgacgg gttcccgaac agaaggcctc ctgggctccc cagagggcga
     8221 agccgacggc cgcggccgta aaggacggcc aggccagcca ggcgcagaag cccagggctt
     8281 tcaggagcat cccgacggcc aggactttcc tcttgtccca gcggtccgcc aggatccccg
     8341 agggcagttc gagcaccact acggatagct cccagaccac gaagagaagg gacagctcga
     8401 ggaaatcgta gccccgttcc cggaacagga tcatgtaggc gggatagatg ggggcaagtt
     8461 ccgacaggaa cgagaagagt acgttctttt tgattatcgg gaagatcgag cgtgcggaga
     8521 aaaacgacat tccttacctc cctatgggtt aacggacgat ccgagcaggt acccgaatct
     8581 cagggaggtc cccgggcgtg aaaaacgcct cgatagcgtc gtcagggtca gtggccggaa
     8641 tagaccttgg atcgcatcat atcctaaagg tagctcgaga tgccacggca ggcaagacaa
     8701 tccgctccgt ccgggattcc ggacccttca gcgcgccctc cgcttggaga tcacgtcgaa
     8761 ggccacggcc agaagaagga ccatgccctt cacgaactgc tggatgtcgc ttcctatgcc
     8821 gaggatggac atcccgttgt tgaggacgcc catgacgagg gcgccgatga tggcccccac
     8881 gatggttccg atgccgccgc tcgcggatgc cccgccgata tagcaggccg cgatggcgtc
     8941 cagctcgaag ttctggcccg ccagggggga ggccgcgttg agacgggagg cgaccgcgat
     9001 tccggccacg gccgaaagca tgcccatgtt gacgtaggcc aggaagagca ccctgttcgt
     9061 gtcgatcccc gaaagctggg cggccttctc gtttccgccc atggcgtaga ggtagcgtcc
     9121 cggcacggtc cgggtggtca ggaaggagta ggccgccacg agggtcccta ttacgatgaa
     9181 gatgacgggg atgccccggt aggaggcgag ccagtaggac gccagggcta tgacgccgac
     9241 ggtcaaggcc agctttgcgg cgaaaagggg caggggcagg ctctcgtagc cgttacgccg
     9301 gcggtagccc cgctggatgg actcggagag cacgaagagc agggccaagg cggctccggc
     9361 ggccagggcc gtccagttgt agagggattc ctcccccccg atcaggtccg gtacgaagcc
     9421 ggagctgatc tgctggaagg actccgggaa aggtccgata gtgagtccct tgagcatgaa
     9481 gaccgtgagc ccgcggaagg tgagcatccc ggccagggtg acgatgaagg cggggatccg
     9541 ggcgtaggct atccagaagc cttgccagac cccgatgacc agccccacgg ccaggaccag
     9601 ggcgatcgac gccccgaccc ccatgtccag ggtgatgatg aaggtgccgc tgagggcgcc
     9661 gatgaaggcg accacggaac ccaccgagag atcgatgttc ccccccgtga ggatgcagag
     9721 ggtcatgccg atggccagaa tgaacacgta cgcgttctgg agcaccagat tggtcacgtt
     9781 catcggcacc aggagaacgc ctcccgtggc gacctggaag aacagcatga tgaagacgag
     9841 ggctatgagc atcccgtact gccgtacgtt gttccggagc agatccttaa tgcttcccat
     9901 ttccgttctc cctgacactc tgcatgatgc acttcatgat gccttcctgg gtggcctctt
     9961 cccgcgggaa ctcgcccacg atccggccgt cgttcatgac gtagatgcgg tcgcagatcc
    10021 cgaggatctc cggaagctcg gaggagatga agaggacgga cttgccctcg tcggccagct
    10081 ggttgatgat cgagtagatc tcgtacttgg cgccgacgtc gatcccccgg gtgggctcgt
    10141 ccaggatgag gacgtccggc atggtgaaga tccacttgga gagcacgacc ttctgctggt
    10201 tcccgccgga caggtctccg gccttctgcc ggatgctcgt ggagcggatc ttgagacggc
    10261 tccggaaatc ctcggccacc ttggcctccc ggtcccggtc gatgacggag cgctcggcca
    10321 ccttccccag gcccgcgatg gtgatgttct cgcagatgtc gccgatcagg ttgagccccg
    10381 cggtcttgcg gtcctcggtg gcgtaggata ctccctggtc gatggtcctg cgcacgctcc
    10441 ggagctcgac ggggcggccg tccttgaagg cgctgcccga gacgcggacg ccgtaggacc
    10501 ggccgaagag gctcatggcc agctccgtgc gtccggcacc catgaggccc gccagtccca
    10561 cgacctcgcc cttccgcagg gtgagggacg cgtccttcac gatcctccga tcgggggaaa
    10621 gggggctgta gacgttccag tcccggatct cgaaagccgt ctcgccgatc cggggctccc
    10681 tcctggggaa tcggtccacg aggtcccttc ccaccatgcc ccggatgatc cgatcctcgt
    10741 cgatgtcgcc cgaggacgtt tccagggtct cgatggtggc cccgtcccgg aggatggtga
    10801 tggagtccgc cacttcgacg acctcgacc
//
LOCUS       DAOTEI010000120        10782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4315_length_10782_cov_5.486250, whole genome shotgun sequence.
ACCESSION   DAOTEI010000120 DAOTEI010000000
VERSION     DAOTEI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4315_length_10782_cov_5.486250"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="P5313_11525"
     CDS             complement(<1..688)
                     /locus_tag="P5313_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY81037.1"
                     /translation="MVNPEGKPRKRLLLVEDEALIALAEKKALEKYRYDVLTVDTGEA
                     AVEAVAGGIDADLVLMDIDLGPGMDGTQAADRILELRDLPILFLSSHTEPEIVERTEK
                     ITSYGYVVKNSSITVLDASIKMAFKLFDADRKTLESERKQNALIANISDVIVIIGRDG
                     TNKYKSPNIHKWFGWEPGEVVGRPALDNVHPDDRAASREILDRLMSSDEGTTATTETR
                     YRCKDGSYKWI"
     gene            complement(708..1271)
                     /locus_tag="P5313_11530"
     CDS             complement(708..1271)
                     /locus_tag="P5313_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY81038.1"
                     /translation="MLIDLSILVTRYRSREALDNEKIAAFGHMGTHFDVMDKEFPLEF
                     LRRKAVVFEVPPDPDREIGVPDIDLSLVGPGMFAAFRTGFIERHEYGSKEYFSGHPQL
                     SEDLIEALLERGVSIIGIDFAGMRRGSEHTPKDQYCADRGVFVVENLCNLDAVLRGRP
                     SASCVMYTFPIKFAGLSGLPCRVAAEL"
     gene            complement(1284..4010)
                     /gene="fdhF"
                     /locus_tag="P5313_11535"
     CDS             complement(1284..4010)
                     /gene="fdhF"
                     /locus_tag="P5313_11535"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRY81039.1"
                     /translation="MSAARTVRFHVDGRSAEAPEGSNLLEALRAAGSEVPSLCWHSRL
                     TPTGACRLCVVKIEGRRGLVTSCSTPVEEGMKVTAFDGELEEARRWILSLLAEEVETG
                     SDGSHRDELEELLLRYGVERPAKLRPSRGAARPPDRSSHVLAFDASRCIKCFRCVKAC
                     LEVQGKGVLSVDGRGLDSVILAGEGTWGASECDGCGECVQLCPTGALTEKPNRDSLRA
                     GGVPVSKVRTTCPYCGVGCQLDLSVREGRILRSDGAEGLSPNDGRLCVKGRFGYGFAN
                     HPDRLTVPLVREGSGFRKATWDEALDRVAAGLNSVRDKYGPDALAGYSSAKCTNEENY
                     LFQKLVRVTFGTNNLDYCTRLCHASTVTAMLKAIGDGAGSASIEDYETADCTLVIGNN
                     MAETHPVTATFLKRGLARGGSLVVIDPKWTPLVRHAEVWLQPRLGTDVALLNGMTRVA
                     FERGWVDRDFARRRIQGGEEALATLRAAVDAYTPERVEAICGVPPDKVLAATRLYARA
                     DRALIATGMGMSQQTVGTDNVFALMNLMLACGKIGRDGSGLAPPRGQNNVQGATDVGC
                     SPLFYPGYVPASDEVNRRRIAELWGVPAVSLPSERGLTTVEIAKAAHEGRIRGLFIMG
                     ENPVMTDPNAFHVEEALEGLEFLAVADIFLTPTSEKADVILPAASWAEKDGTFVNSDR
                     RVLRVRRAIRSPGEAREDLWILQELARRMGRPIGTYRSASEVWDETARAAPAIFGGIT
                     WSRLEERGIQWPCPRADHPGTRTLFLERFNTPDGLARVFPVDWRPQAELPDAEYPLVL
                     NTGRILYQYHSSTMSRRAGPLDAFAPEGYILLHPEDARRIGVGDGDRVRVTSRRGGLV
                     TRARSSEEVREGEPFMPFHYEAARVNILTRDELDPSSKIAPFKNTPVRVEKA"
     gene            complement(4007..5770)
                     /locus_tag="P5313_11540"
     CDS             complement(4007..5770)
                     /locus_tag="P5313_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY81040.1"
                     /translation="MIRPDRLLEDLHGIQEVSGRNWLPPAELDRLGLRYGKLRGDIRG
                     VASYYSMLSLEPRGRHVIRLCVSPVCRMMGSEDLLAFLRAELGVESGGTTRDGLFTLE
                     ECQCLGQCGRSPAMMVDDKVFTRLTPVSVRAALDLYRIAGAADRSASAPSALEPESAR
                     GAASSCPGETRIALRYIDLHDPGDLGSYEAAGGWAGLRAALGKDPDDLVADLTKAGLR
                     GRGGAGFPTGTKERSTAQTCEDCDRYVVVNADEGEPGTFKDRIILEREPQLLLEGLVI
                     SAYAIGARMGYIYVRGEYGRSIRNLRTAIDQAKAAGYLGENILGSGFDFDVEIRRGAG
                     SYLCGEELTLLESLEGKRGYPRIKPPFPAEKGLWGKPTLVNNVETLANVPWIAENGAD
                     AYLALGVPGSPGTKIFCVSGDVVRPGFYEAELGISLRDLVDGFAGGISPAPDGSVRSV
                     REPAAVLLGGAAGTFASAARMDLRLDYDSLKKEGLTLGSGAVIVLGPGRDPADTILSI
                     LEFFRHESCGKCVPCRVGTARLAEAGRELAAAKPGERRGILDALVVEARRMAATSLCP
                     LGQSPILPLAAAARELREALS"
     gene            5896..7320
                     /locus_tag="P5313_11545"
     CDS             5896..7320
                     /locus_tag="P5313_11545"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY81041.1"
                     /translation="MVRKFGFVRWARAMALVLASLVLVSPIVSQERTDASLASELDAY
                     VRGMVSSGTFTGTVLAARGGRILLCKGYGMADRERGIRNDIRTRYRLGSVSKNFTAAA
                     VLLLQERGALSIDDPISRYLPGLFEGKGITIRHLLTHTSGLPDYILFPGAFEAFAEAT
                     DPDALLASIAAPDLEFEPGTKFRYSNSGYVVLGRIVEVVSGMEYEDFLGTELFRPAGM
                     SDAGCDYEEGLAAGDVAVGYHYTGEDYVPVPRWDSSWAAGAGAVCASAVDLYSWSRAL
                     ARGRILSDRSRDLLFGPTPQSRNAYGMGWYLRPNDCRARTYHGGYVFGFRSDFTRYPG
                     EEADIIVLSNVDQAPTRRMAEDLAAILFGIPYDPPEFRVPASLPSSGLKNFEGVYDTS
                     EIFGDGASMQVWTEGPRIWYRGNTVYDSLGLPMHIYPLEGGGFFDKTGDVKYEFIPGP
                     DGAAEAVVVSDAYESYRFPRIPSR"
     gene            7317..8480
                     /locus_tag="P5313_11550"
     CDS             7317..8480
                     /locus_tag="P5313_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRY81042.1"
                     /translation="MRARSALIALALVCAVLTSTDAQTAAPAPLPLPSAIHPLLARGR
                     AAILESPGTLITVGRVGCRFPTLKAALEASPGGNARICLMDTVHRESGIVIRGNVEIF
                     GFGAVGTVLEAAARPEEAGDRVIRVEEGACLYLSGLTVRGGRPSDRLRNGGGIANYGR
                     LVIEDCAVIDNQSSAGAGIWSAGPLEIRRSVIAANRTVPRPAADATAAVGCQGSGAGI
                     KVDKPGSARIEDTLIAWNASLKGGGGLHVACETEVRLSRCTIFGNSSKTRGGGIDLAG
                     GTLILERCTVAGNVSTGKGFAVHNRGRLSLSGCLLAGEYGTAYYRAVDSGGEFGSGAL
                     VLNEGNYCQSGNLPGSAVGDPGPLVLADHGGPVWTVLPDPSSPARGYGAGPLP"
     gene            complement(8563..10122)
                     /locus_tag="P5313_11555"
     CDS             complement(8563..10122)
                     /locus_tag="P5313_11555"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRY81043.1"
                     /translation="MNHFRILGAACLALALGSCSPADRPAPGTDPKIVLYAEFMHEVN
                     SFSPILTTERDFRAMALRYGAEVAAAAPGEKTQLAGFLKAVEKSGAGAVKAVPVLQAK
                     SMSGGPVDRAFYERIKKEILDAVKAADRVDGVYLSLHGAMGVDGIFDPEGDILVDLRT
                     LLGPEVPVAVSFDLHANVTKRRVENTDIIVGYRTNPHRDHFDTGRRTGDLLVRTVLGR
                     IDPVMRVNKMALLKGGGMNVDFLPPFRKIFSALKRMERKGGALSVSFFPVHIWIDEPE
                     LGYSTVAITDGDPDRAAALADEISDMAWAVRDVPQPAGATPEEAVDLARKAGLARALG
                     TVVFCDVSDAVGTGTPGESVWILKALLERGSDLVSYLSIRDEKAAALAWDRKVGDEVT
                     LSVGGKIDLLYNRPLEYSGIVEWKAETSFGKTVIVRRDGIRLVISELPMASRTPKEFK
                     SLGLDPWKADIVVVKNLFPFRYNFLLYNRKTVNVMSPGLSNTDVFGLKYRNIPRPIYP
                     LDKVEDWRIEP"
     gene            10257..10712
                     /locus_tag="P5313_11560"
     CDS             10257..10712
                     /locus_tag="P5313_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY81044.1"
                     /translation="MSTFAALLTAGLLGATISIYQPMNGLVSRITGSPLAANTLFYFV
                     AFLSSAAMMSVWTLVRPGAPGLASRLLELPPALLLAGSMSSVMVLGTILLLPRLGARR
                     LFLLQVAGQVIMALAVSHFGLLGTARDPLTLRKALGAVLLLAGAVVSVL"
BASE COUNT         1627 a         3778 c         3601 g         1776 t
ORIGIN      
        1 ggatccactt gtaggaaccg tccttgcaac ggtatcgcgt ctccgtggtc gcggtcgtcc
       61 cttcgtccga ggacatgagc cgatcgagga tttcccggga ggcggcccgg tcgtcggggt
      121 gcacgttgtc caaagccggc cttcccacga cctccccggg ttcccagccg aaccatttgt
      181 ggatgttcgg actcttgtac ttgttggttc cgtcgcggcc gatgatcacg atcacgtcgg
      241 agatgttggc gatcagggcg ttctgcttgc gctcactttc cagggtcttc cgatcggcgt
      301 cgaagagctt gaaggccatc ttgatggagg cgtccaggac ggtgatactg gagttcttga
      361 ccacgtagcc gtaggaggtg atcttctccg tcctctccac gatctccggt tccgtgtggg
      421 aggaaaggaa gaggatcggc aggtcccgca actccaggat ccggtccgcc gcctgggttc
      481 cgtccatgcc cggtcccagg tctatgtcca tgagcacgag gtccgcgtcg atcccccccg
      541 cgacggcctc gaccgcggcc tccccggtgt cgaccgtaag gacgtcgtag cggtatttct
      601 ccagcgcctt cttttccgcc agggcgatca gggcctcgtc ctcgaccagg aggagtctct
      661 tccggggctt tccttccggg ttcaccatgg acacctcgcc gaccgcatca tagctccgcc
      721 gccacccggc agggcaggcc ggagagcccc gcgaacttga tcgggaaggt atacatcacg
      781 caggacgcgg agggccgtcc ccgcaggacc gcgtccaggt tgcagaggtt ctccaccacg
      841 aagacgcccc ggtccgcgca gtactggtcc ttgggggtat gctcggatcc ccggcgcatc
      901 cccgcgaagt cgatgccgat gatcgaaacc ccgcgttcca ggagggcctc gatcagatcc
      961 tccgaaagtt gggggtggcc ggagaaatat tccttggagc cgtactcgtg ccgttcgatg
     1021 aagcccgtcc ggaaagcggc gaacatcccc ggtccgacca gggacaggtc gatatccggg
     1081 accccgatct cccgatccgg atccgggggg acctcgaaca cgaccgcctt cctcctcagg
     1141 aactccaggg ggaactcctt gtccatcacg tcgaaatgcg tccccatgtg cccgaaggcc
     1201 gcgatcttct cgttgtccag ggcttccctg ctccggtacc gtgtcaccag gatgctcaga
     1261 tcgatcagca tgtctccctc tccctaggcc ttttccaccc gcacgggggt attcttgaag
     1321 ggcgcgatct tggaggacgg gtccagctcg tcccgggtca ggatgttgac ccgggccgcc
     1381 tcgtagtgga agggcatgaa gggttcaccc tcccgcacct cttcgctgga gcgcgcccgc
     1441 gtcacaaggc cgccccgccg ggacgtcacc cgtacccggt ccccgtcccc gaccccgatc
     1501 cgccgggcgt cctcggggtg caggaggatg tacccctccg gcgcgaaggc gtccaggggc
     1561 ccggccctgc ggctcatggt ggaggaatgg tattggtaca ggatgcgtcc ggtgttcaag
     1621 accagaggat actccgcgtc cgggagttcc gcctggggcc gccagtcgac cgggaagacc
     1681 cgggccaacc cgtccggggt gttgaaccgc tccaggaata gggtgcgggt gcccggatgg
     1741 tccgccctcg ggcagggcca ctggatgccc cgctcctcca gtcgggacca ggtgatcccc
     1801 ccgaagatgg cgggcgcggc ccgggcggtc tcatcccata cctccgaggc ggacctgtac
     1861 gttccgatgg gccgtcccat ccggcgcgcg agctcctgca ggatccagag gtcttcccgg
     1921 gcctctccgg gcgaccggat cgcccgtcgg acccgcagga ccctccggtc cgaattgacg
     1981 aaggtgccgt ccttctcggc ccaggaggcc gcggggagga tcacgtcggc cttctccgac
     2041 gtcggggtca ggaagatgtc cgccaccgcc aggaactcga ggccctccag ggcctcctcc
     2101 acgtggaagg cgttcgggtc cgtcatcacg ggattctccc ccatgatgaa cagtccccgg
     2161 atccggcctt catgggcggc cttggcgatc tccacggtgg tcaggccccg ttccgaaggc
     2221 agggacaccg cgggcacgcc ccacaactcc gcgatccggc gccggttgac ctcgtccgag
     2281 gcggggacgt agcccgggta gaacaggggc gagcagccca cgtcggtggc cccctgcacg
     2341 ttgttctgac cccggggcgg ggccaggccc gatccgtccc ggccgatctt tccgcaggcc
     2401 agcatcaggt tcatcagggc gaagacgttg tcggtgccca cggtctgctg gctcatcccc
     2461 atgcccgtcg cgatcagggc gcggtccgcc cgggcataga gccgggtcgc ggccaggacc
     2521 ttgtccgggg gcaccccgca gatcgcctcc acccgttccg gcgtgtaggc gtccacggcg
     2581 gcgcgcaggg tagccagagc ctcctcgccc ccctggatgc gccgccgggc gaagtcccgg
     2641 tccacccagc cgcgttcgaa ggcgacccgg gtcatcccgt tgagcagggc cacgtcggtg
     2701 cccagccgcg gctggagcca gacctccgcg tgccggacca ggggggtcca cttggggtcg
     2761 atgaccacca gggacccgcc ccgggccagg ccgcgtttta ggaaggtggc cgtgacggga
     2821 tgggtctccg ccatgttgtt gccgatcacg agggtgcagt ccgcggtctc gtagtcctcg
     2881 atggaagcgc ttcccgcccc gtccccgatg gccttgagca tggcggtgac cgtggaggcg
     2941 tggcagagcc gggtgcagta gtccaggttg ttggtgccga acgtgacccg cacgagcttc
     3001 tggaagaggt agttctcctc gttggtgcac ttggcgctgg agtagcccgc cagggcgtcg
     3061 ggaccgtact tgtcacgtac cgagttcaac cccgccgcga cgcggtccag ggcctcgtcc
     3121 caggtagcct tccggaagcc ggaaccctcc cggacaaggg gtacggtcag ccggtcggga
     3181 tggttcgcga agccgtagcc gaagcggccc ttgacgcaga gcctgccgtc gttgggggag
     3241 agtccctccg ccccgtcgga tcggagtatc cggccctccc ggacgctcag gtccagctgg
     3301 cagcccaccc cgcaataggg gcaggtggtg cggactttgg atacgggaac ccctccggcg
     3361 cggagggagt cccggttcgg tttttccgta agggctccgg tcgggcagag ctggacgcac
     3421 tccccgcagc cgtcgcactc cgaggccccc caggtcccct ccccggccag gatcaccgag
     3481 tccaggcccc ggccgtccac ggacaggacg cccttgccct ggacctccag gcaggccttg
     3541 acgcagcgga agcacttgat gcaccgggac gcgtcgaagg caaggacgtg ggaactccgg
     3601 tccgggggac gggcagctcc ccgggagggc cggagcttcg cgggcctctc gaccccgtac
     3661 cggaggagaa gctcctccag ctcgtccctg tggctgccgt cggacccggt ctcgacctcc
     3721 tcggccagga gggaaaggat ccagcggcgg gcttcctcga gctccccgtc gaaggcggtg
     3781 accttcatgc cctcttccac cggggtcgag caggaggtga cgagcccacg gcggccctcg
     3841 atcttgacca cgcagagccg gcaggccccg gtgggggtga gcctggaatg ccagcagagg
     3901 ctgggcacct cggagcccgc ggcccgcaga gcctcgagca ggttcgagcc ttccggggcc
     3961 tcggctgacc tgccgtccac gtggaagcgt accgtccggg ccgcgctcac gacagggcct
     4021 cccggagctc ccgggcagcg gccgccaggg gcaggatcgg ggactgcccc agggggcaga
     4081 gggaagttgc ggccatccgc cgggcctcca ccaccagggc gtccaggatc ccccggcgct
     4141 cgccgggctt cgcggccgcc agttccctgc ccgcctccgc gaggcgcgcc gttcccaccc
     4201 ggcagggcac gcacttgccg caggattcgt ggcggaagaa ctccaggatg gacaggatcg
     4261 tgtccgccgg atcccgcccc gggcccagca cgatgaccgc cccggaaccc agggtgagcc
     4321 cctccttctt gagggagtcg tagtcgaggc ggaggtccat ccgggcggcg gaggcgaagg
     4381 ttcccgccgc cccgcccagg aggacggcgg ccggttcccg caccgaccgg accgaaccgt
     4441 ccggcgcggg ggaaatcccg cccgcgaatc cgtccaccag gtcccgcagg gagatgccca
     4501 gctcggcctc gtaaaaaccc gggcggacca cgtccccgga gacgcagaag atcttggttc
     4561 ccggagaacc cggcactccc agggccagat aggcgtcggc cccattttcg gcgatccagg
     4621 gcacgttggc cagggtctcc acgttgttga ccagggtggg cttgccccag agccccttct
     4681 ccgcggggaa gggcggcttg atccggggat acccccgttt gccttccagg gattccagga
     4741 gggtcagttc ctccccgcag aggtaggagc ccgcgcccct ccggatctcc acgtcgaagt
     4801 cgaagcccga gcccaggatg ttctccccca gataccccgc ggccttggcc tggtcgatcg
     4861 cggtccggag gttccggatg gagcggccgt actccccccg gacgtagata taccccatcc
     4921 gggccccgat cgcgtacgcc gagatgacca ggccttccag gagaagctgg ggctcccgct
     4981 ccaggatgat ccggtccttg aaggttcccg gctcgccctc gtcggcgttc accaccacgt
     5041 accggtcgca gtcctcgcag gtctgggccg tggaccgctc cttggtgccc gtggggaagc
     5101 cggcccctcc ccgcccccgg agcccggcct tcgtgagatc cgcgaccaga tcgtcggggt
     5161 ccttcccgag ggccgcgcgg agcccggccc agccgccggc ggcctcgtag gacccgaggt
     5221 ccccgggatc gtgcagatcg atgtaccgca gggcgatccg agtctccccg gggcaggagg
     5281 aagccgcgcc gcgggcggat tccggttcca gggccgacgg agcggaggcg gagcgatccg
     5341 cggcgcccgc gatccggtac aggtccaggg ccgcccggac ggacacgggg gtcaagcggg
     5401 tgaatacctt gtcgtccacc atcatggccg gggaacgccc gcactgcccc aggcactggc
     5461 attcctccag ggtgaacagg ccgtcccggg tcgtccctcc ggactcgaca cccagttcgg
     5521 cacgcaggaa cgcaagcaga tcctcggagc ccatcatgcg gcagaccggg gacacgcaga
     5581 gccggatcac gtggcggccc cggggctcga ggctgagcat ggaataatag gacgcgaccc
     5641 cccgaatatc cccccggagc ttgccgtatc tcaggcccag ccggtccaat tccgccggcg
     5701 gcagccaatt tcggcccgag acttcctgga taccgtgcag gtcctcgaga aggcgatctg
     5761 ggcgtatcat ggaataagta tagacgcaat tcgcgggcag gcaatatgca tcggttttcc
     5821 ttgacgcccc gcccgaaggg gaggacaatt catcgatccc ccggtattcc aggattccta
     5881 tggggaggtg cgccgatggt acggaaattc ggattcgtcc gctgggcgcg ggcgatggcc
     5941 ctggtcctcg cttccttggt tctggtttct ccgatcgttt cccaggaacg gacggatgcc
     6001 tcgctcgctt cggagctgga cgcctacgtg cgcgggatgg tgagctccgg aaccttcacg
     6061 ggtaccgtcc ttgcggcccg gggaggccgc atcctcctgt gcaagggcta cgggatggcg
     6121 gaccgggaac ggggtatccg gaacgacatc cggacccggt accggctggg ctccgtctcc
     6181 aagaacttca ccgcggcggc cgtcctgttg ctccaggagc ggggagccct ttcgatcgac
     6241 gacccgattt cccggtacct tccggggctg ttcgaaggga aggggatcac gatccggcac
     6301 ctgctcaccc atacgtccgg acttccggat tatatcctgt tccccggggc cttcgaggcc
     6361 ttcgccgaag cgaccgatcc ggatgcgctc ctggcctcca tcgcggcgcc ggacctcgag
     6421 ttcgaaccgg ggacgaagtt ccggtacagc aattccgggt acgtggtcct cggccggatc
     6481 gtcgaggtgg tatccgggat ggagtacgag gattttctcg ggaccgagct gttccgtccc
     6541 gccgggatgt ccgacgccgg ctgcgactac gaggaggggc tcgcggcggg ggatgttgcc
     6601 gtggggtacc actacaccgg agaggactat gttcccgtgc cccgctggga ttcctcctgg
     6661 gcggccggcg ccggtgcggt ctgcgcgagc gccgtggacc tgtactcctg gagccgggcc
     6721 cttgcccggg gcagaatctt gagcgaccgt tcccgggacc tcctgttcgg ccccactccc
     6781 cagagccgga acgcctacgg catgggctgg tacctgcggc cgaacgactg cagggcccgg
     6841 acctaccacg gcggctacgt cttcggattc cgcagcgact tcacccgata cccgggagag
     6901 gaagccgaca tcatcgtgct cagcaacgtc gaccaggcgc ccacccggcg gatggcggag
     6961 gacctcgccg cgatcctttt cgggattccc tacgacccgc cggagttccg ggtgcccgca
     7021 tcgcttccga gttccggtct caagaacttc gagggcgtgt acgacaccag cgagatcttc
     7081 ggggacggcg cgtccatgca ggtctggacg gagggccccc ggatttggta ccggggaaac
     7141 acggtctacg attccctggg actgcccatg cacatctatc ccctcgaggg aggaggcttc
     7201 ttcgacaaga ccggggacgt caagtacgaa ttcatcccgg ggccggacgg cgccgcggaa
     7261 gccgtggtcg tgagcgacgc ctacgaaagc taccgcttcc ccaggatccc ctcccgatga
     7321 gggcgcgatc cgccctgatc gccctggcgc tggtctgcgc ggtcctgacc tcgacggacg
     7381 cccagactgc ggctcccgcc ccccttccgc ttccgagcgc gatccacccg ctcctggccc
     7441 ggggccgcgc cgccatcctg gaatcgccgg gaaccctgat cacggtcgga cgggtcggct
     7501 gccgtttccc gaccctgaag gccgcgctgg aggcgagtcc cggcggaaac gcgcggatct
     7561 gcctcatgga caccgtgcac cgggaaagcg ggatcgtcat ccggggtaac gtggaaatct
     7621 tcggcttcgg cgccgtaggg accgtcctgg aggccgcggc gcgtccggag gaggccgggg
     7681 accgggtcat ccgggtcgag gaaggcgcat gcctgtacct ctcgggcttg acggtccggg
     7741 gagggcgccc ctccgaccgg ctgcgcaacg gggggggcat cgccaactac gggcgcctgg
     7801 tcatcgagga ctgcgcggtg atcgacaacc agtcctccgc gggagcggga atctggtcgg
     7861 cggggcccct ggagatccgg cggtccgtga tcgccgcgaa ccgcaccgtc ccgaggcccg
     7921 cggcggacgc cacggccgcc gtgggctgcc agggcagcgg ggcggggatc aaggtggaca
     7981 agccgggatc cgcccggatc gaggacaccc tgatcgcgtg gaacgcctcc ctgaagggcg
     8041 gaggcggtct ccacgtggcc tgcgagaccg aggttcgcct ttcccgctgt acgatcttcg
     8101 gcaattcgtc caagacccgg ggcgggggaa tcgatctggc cggggggacc ttgatcctcg
     8161 agcgctgcac cgtggccggc aacgtctcga ccggaaaagg tttcgccgtc cacaatcgcg
     8221 gccggctttc cctgtccggc tgcctgctcg ccggtgaata cggaaccgcg tactaccggg
     8281 ccgtcgattc cggaggagag ttcgggtcgg gggccctggt gctcaacgag ggcaactatt
     8341 gccagtccgg caaccttccc ggctcggccg tgggagaccc cggccccctc gtcctcgcgg
     8401 accacggcgg acccgtctgg accgtcctgc cggatccgtc ctccccggcc cgggggtacg
     8461 gggccggtcc cctgccctag ccgctgcgat cctttcccgg cccggtttcg acgcggtgac
     8521 cccggccggg gcatcgcgtc cgccgggccg gagtccagcc ttctacggct cgatccgcca
     8581 atcctccacc ttgtccaagg ggtagatggg tcgggggatg ttccggtatt tcaggccgaa
     8641 gacgtccgta ttgctcaggc ccggggacat cacgttcacg gtcttgcggt tgtacaggag
     8701 gaaattgtac cggaagggga agaggttctt gacgaccacg atgtccgcct tccaggggtc
     8761 cagccccagg ctcttgaact ccttgggggt cctggaagcc atggggagtt ccgagatcac
     8821 cagccggatc ccgtcccggc ggacgatgac ggtcttcccg aaggaggtct cggccttcca
     8881 ctccacgatg ccggaatact ccaggggccg gttgtacagc aggtcgattt ttccgcccac
     8941 gctcagggtc acctcgtccc cgaccttgcg atcccaggcc agggcggcgg ccttttcgtc
     9001 ccggatcgag aggtacgaga ccaggtcgct cccccgctcc aggagggcct tgaggatcca
     9061 cacgctttcc ccgggcgtgc cggttccgac cgcgtcggac acgtcgcaga agaccacggt
     9121 gcccagggcc cgggcgagcc cggccttccg ggcgaggtcc acggcctctt ccggggtggc
     9181 gcccgcgggc tggggcacgt cgcggaccgc ccaggccata tcggaaatct cgtccgccag
     9241 ggcggccgcc cggtccgggt cgccgtccgt gatcgcaacg gtgctgtacc ccagctccgg
     9301 ctcgtcgatc cagatgtgca ccgggaagaa ggagacggaa agggccccgc ccttacgttc
     9361 catcctcttc agggcgctga agatcttccg gaagggaggc aggaaatcca cgttcatccc
     9421 gccccccttg aggagggcca tcttgttgac ccgcatgacc ggatcgatcc ggcccaggac
     9481 cgtgcgcaca aggaggtcgc cggtccggcg gcccgtgtcg aagtggtccc ggtggggatt
     9541 ggtccggtac cccacgatga tatccgtatt ctccaccctg cgcttcgtga cgttcgcgtg
     9601 caggtcgaag gacacggcaa ccggcacctc cggccccaac aaggtgcgca ggtcgacgag
     9661 tatatccccc tcggggtcga agatcccgtc cacgcccatc gccccgtgga gggacaggta
     9721 gaccccgtcc actcggtccg ccgccttcac ggcgtccagg atctccttct tgatccgctc
     9781 gtaaaaggcc cggtccacgg gtcccccgga catggacttg gcctggagca cgggaacggc
     9841 cttcacggca cccgcgccgg atttctccac ggccttgagg aagcccgcca actgggtctt
     9901 ctcccccggc gcggcggcgg cgacttccgc tccgtaccga agggccatgg cccggaaatc
     9961 cctctccgtg gtcaggatgg gggagaagga gttgacctcg tgcatgaact ccgcgtacag
    10021 cacgatcttc gggtccgtcc cgggggcggg acggtcggcg ggggaacagg agcccagggc
    10081 aagggccagg caggccgcgc caagtatccg gaagtggttc atggtccgta acactaggct
    10141 acccggatcc tcctgtcaac gcggccgcgc ggccggaccg ggggctgact cgggccgcct
    10201 tcccgaagta ccattcgaaa gatccggcgc gcggccggcc ctaggagcga acagccatgt
    10261 ccaccttcgc ggccctactc accgcgggcc tcctcggagc caccatctcg atctaccagc
    10321 ccatgaacgg cctcgtgtcc cggatcacgg gctcccccct ggcggccaac accctcttct
    10381 acttcgtggc cttcctctcc tccgcggcca tgatgtcggt ctggaccctg gtccgcccgg
    10441 gcgcccccgg actggcctct cggcttctgg agctccctcc cgccctgctc ctggcgggca
    10501 gcatgagctc cgtcatggtc ctggggacga tcctcctcct gccgcgcctg ggggcgcggc
    10561 gcctcttcct cctgcaggtg gcgggtcagg taatcatggc cctggcggtc agccatttcg
    10621 ggctcctggg tacggcccgg gatcccctca ccctgcgcaa ggccctgggc gccgtcctcc
    10681 tgctggccgg agccgtcgtc tcggtgctct gatccttcgc cccgccttgc ccgcggccga
    10741 tccggtaagt acctttgacc cgggccgggt catccggccg gg
//
LOCUS       DAOTEI010000121        10722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4343_length_10722_cov_5.000656, whole genome shotgun sequence.
ACCESSION   DAOTEI010000121 DAOTEI010000000
VERSION     DAOTEI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10722
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4343_length_10722_cov_5.000656"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="P5313_11565"
     CDS             <1..1516
                     /locus_tag="P5313_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY81045.1"
                     /translation="ADGKIRAQEMTMYWDSGAYADYAVNVTRASGYSAAGPYEIPNAS
                     IDAYTIYTNKPFGTAYRGFGHVEFHWGLERHMELVAKAIGMDPLEFRRRNALRPGSIT
                     LTGERITDHTGDVVKCMDAAAKAIGYGSLTAEEKERERKTGRRIGKGVAALHKAPAMP
                     SFTATAVIIKMNEDGSVTVNLALTDYGQGTYTSIRQIVAERLGFPLEKVKVSFECDTD
                     KDPYDWQTVASKGLLLSGNAAILAAEDLLKNAYEVAAQVLRANVRDLAHDGKRIYLRH
                     NPAESVDFARIAVGYAYPNGNGIGGPIIGVGRYVAQGLSNLDKATGQGNPALDWTYGA
                     HGIVAEVDPETGEYEVLKIASVFDVGKAINPQIVRGQSIGGMVQGLGTAVCEGYIYDE
                     KGILLNPSFTDNKIPTAKDIPAVIESAVVETPQIDGPYGARGVGEHSMISVAAALGNA
                     IQEATGAEIVHMPLRFEDVWRALRDKTPIDNWITKSPLGSCRSAPELRNRKKDG"
     gene            1689..2942
                     /locus_tag="P5313_11570"
     CDS             1689..2942
                     /locus_tag="P5313_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRY81046.1"
                     /translation="MEKSLTVKNTVLGIQHTFVMFGATVLVPIITGLDIGVTLFAAGL
                     GTLLFHVLTGFKVPVFLGSSFAFIPGLLAVGKSQGLPYALGGIVIAGLLYVVVAIIFK
                     FVPYDTIHKILPPHVTGPMIILIGLILAPVAIQNARGDFSKPIVDAIGVNGCWAVALF
                     TFAVGIFVKIYLPKFGMKFASNLPVLIALVAGYVLSIILGIVDFSAVAKADWIGIPKF
                     TLPKFSAEGISIMLPIAIVTMVEHFGDILAIGNVVGKDFIKDPGISRTLLGDGLATSL
                     AAMIGGPANTTYSENTGAVALTGAYNPIIMRIAAVCAIILAMIPKFTAIIGTIPGPVI
                     GGISILLFGMISSIGVKNMVDARVNLGNAKLLIITAAMLVLGLGGATFELGALKLSGL
                     GLAAIFGIVLNLILRPKDITSAECD"
     gene            2947..3813
                     /locus_tag="P5313_11575"
     CDS             2947..3813
                     /locus_tag="P5313_11575"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HRY81047.1"
                     /translation="MNPRALAGPLVPREAWEARAEGVFRGAIALAHPDGLWVTILRSR
                     EDMEPRGIWPGPEAFDALAGLVVPGRPVVFSGEADPFLAAPGGTLRTGPLESWDPRPE
                     LADAVAAFREAGPSAPSAAAELMRAALATDGYTEGLHGEGPFGRRFAELRKEPGFPCN
                     LAGFGPGTTPAGDDFLAGWCLGGILRGEGESVLLRFAGIDGSRTTVPGRTLLAGAARG
                     LFPAYLVRLASALAAAVSGGAGPAREGDRDGPDARGRREDILASAARAAFSHGASSGR
                     DAMLGLMEGVGG"
     gene            3810..5393
                     /gene="fdrA"
                     /locus_tag="P5313_11580"
     CDS             3810..5393
                     /gene="fdrA"
                     /locus_tag="P5313_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HRY81048.1"
                     /translation="MKLLRVRKDSYHDSVFLMLATQELKASGGVLDAVVAMGTDMNRE
                     LLSDMGFSGPDLDGAGPNDLVVAVEAGDAAKAEAAAARALELVDRKKSAAAASGGTGL
                     ARSGSLDAALGRLPGANLAVISLPGAYAAREARKALDRGLHVMLFSDNVPLEEEIALK
                     RHASAKGLLVMGPDCGTAIVNGQPLCFANVVRRGTVGVAAASGTGLQEVTCLVDRYGA
                     GISQAVGTGGRDLKNAAVGGRTMLLAVAALAADPATSVIVVISKPPAPDTALAVMEAL
                     SRSGKPCVVHLLGLKDPAVLGKPAANVHLASDLEDCARRAAALARGEPHRPVEWDLSE
                     AEIERIVEREASGFAPAQRHLRGLYTGGTLADEALFLLQPLLGPVWSNNQTDPALVPP
                     DPQVSTGHTIVDLGDDVFTVGRPHPMIDPSTRTDRLDKEAEDPSVAVLLLDLVLGYGS
                     HPDPAGALAPRLEAARAKAAARGGRLSVVASVTGTPSDFQGLEDQKRKLEAAGAVVMP
                     SNRQAARLAARILQKGAAR"
     gene            5390..6826
                     /locus_tag="P5313_11585"
     CDS             5390..6826
                     /locus_tag="P5313_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HRY81049.1"
                     /translation="MSAEPMNVKPVLGLFSGDLAVVNLGLEGFAETLAGRGVPTVQVD
                     WKPPAGGRKDVIEALDAIARNPRVAEANAEALSRILAGKPTLVGIGTALDTVPGMARD
                     LILHAGPPVAWKDMAGPLRGAVIGGLLYEGLASTPEEAQTLAASGKVRFEPCHHHSAV
                     GPMAGVMTASMPVWIVENQAFGNRAYCTLNEGLGKVLRYGAYSREVLDRLRWMAERLA
                     PVLAKALALRGPIDMKSLIAQVLQMGDEGHNRNRAGTSLVIRELAPHLALLDEPKEDI
                     SKVFSFLNGNDHFFLNLTMPSAKCTVDAALGIEASSVIATMARNGTEFGIRVSGLGER
                     WFTGPASVVEGLYLPGFSAADAARDVGDSVITETSGIGGFAMAAAPAIVKFVGGTPED
                     ALNVTRRMYEITVGENDAYRIPALDFRGTPTGIDVCKVVETGILPAINTGIAHREPGI
                     GMVGAGLVKPPANCFTDAVLAFAEKYGN"
     gene            7082..9184
                     /locus_tag="P5313_11590"
     CDS             7082..9184
                     /locus_tag="P5313_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY81050.1"
                     /translation="MKKGAGKMILLVEDEALISAATSRTLQRFGFTVRTAPTGERAVE
                     AVETDPDIDLVLMDIDLGPGIDGAEAARRILRIRDLPLVFHSAHTEPEVVEKTEDITS
                     YGYIVKNSGDTVLGASIRMAFRLWESEAKFRTAFESVAVGMVLTSPDGKLLRVNQEFC
                     SMLGYTREELASLDFAALTHPDDVETSRARMTALRNGESDRTRFKKRYVHKDGREIWA
                     DLSVVLLRDSAGKPLHFVTHIQDITEDRRIREELERSRSRFDLLLRSVPASILVVQDG
                     RYVFANPYGARLLGYESPENLVGTLQFDSIDVESREAVKLRMARIALGESNDPRELIV
                     LRKDGTRVTTESASLPIEYDGRPAALILGRDKTEQKAVEGRLASILKAVPTGIGVVQG
                     PDREIREVNDKLCEMTGYSREELEGRSIALFYADPAEYERIGREKYRQMEERGYGTAE
                     TVWRHKDGRTLDVFLASAPLYPGDLSRGVTFAALDITERKRAEEAVRSLLQEKDLLLR
                     ETHHRVKNNLGLVHSLLSLQAGRQADPAARGVLGDAAVRVQAMSRLYDRLYRSENFRE
                     MSLKSFLPGIVDQVRDLFRLDAEVRADLRIEDIPLPVRTLTPLGIILNELVTNSMKYA
                     FQGRPEGRITLTALRDGPVVRITYGDDGPGLPESFNLDTPAGFGLQLCGILSGQLGGT
                     LRLERDRGTRFVLEFPAN"
     gene            complement(9215..10441)
                     /locus_tag="P5313_11595"
     CDS             complement(9215..10441)
                     /locus_tag="P5313_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY81051.1"
                     /translation="MDRKPVELTRKAAVALIVLFGIVSLFADMNYEGGRSVVGQYLKL
                     LGTSAFALGLSAGLGEFVGYALRLVSGSIADRTRKYWTLIILGYVVQLCSLPMLAFVR
                     GWELAVVFLFLERVGKAIRKPAHDAVVSFAAKQTGSGFGFGLHEAMDQIGAFLGPALF
                     SALLFLGPDAKTLAGYRTGLLLLFIPAALAVGFTVYNRFLFPHPERFELKKSAVEVGT
                     SGYSRSYWIIVLAAGLLAMGITDFPLIGLHLKRGAGFPEEAIPLLYAGAMAVDAAAAL
                     VFGRWYDKAGLKVLAVLFAAELFTAPLVFLGGPAAILVGMALWGISVGTQESILKAAI
                     GDRVPEDRRARAFGLFNTLFGLAWFAGSAVIGLLYDRFGAVPLVAFSVAVQGAALVVF
                     LGLVRGPGREARPKTE"
BASE COUNT         1800 a         3647 c         3652 g         1623 t
ORIGIN      
        1 ggcggacggg aagatccgag cccaggagat gaccatgtac tgggactccg gggcctacgc
       61 ggactacgcg gtcaacgtga cccgtgcctc gggctactcc gccgcgggac cctacgagat
      121 ccccaacgcc tccatcgacg cctacaccat ctacacgaac aagcccttcg gcaccgccta
      181 ccggggtttc gggcacgtgg agttccactg gggcctggaa cggcacatgg agctggtggc
      241 caaggccatc ggcatggatc cccttgagtt ccgccggcgg aacgccctcc ggccggggtc
      301 gatcaccctg acgggggaga ggatcacgga ccacaccggg gacgtggtca agtgcatgga
      361 cgccgccgcg aaggccatcg gctacggctc cctgaccgcg gaagagaagg agagggagcg
      421 gaagacgggc cggaggatcg gcaagggcgt cgcggccctg cacaaggccc ccgccatgcc
      481 ctccttcacg gccaccgccg tgatcatcaa gatgaacgag gacgggagcg tcacggtcaa
      541 cctggccctg accgactacg gccaggggac ctacacctcc atccggcaga tcgtggcgga
      601 gcgtctgggc ttccccctgg agaaggtgaa ggtctccttc gagtgcgaca cggacaagga
      661 cccctacgac tggcagaccg tagcctccaa gggcctcctg ctttccggga atgccgccat
      721 cctggccgcc gaagacctcc ttaagaacgc ctacgaggtc gccgcccagg tccttagggc
      781 caacgtccgg gacctggccc acgatggaaa acggatctac ctgcgccaca accccgcgga
      841 gtccgtggac ttcgcccgga tcgccgtggg ctacgcctat cccaacggga acggcatcgg
      901 ggggccgatc atcggggtgg gccgctacgt ggcccaaggg ctctccaacc tggacaaggc
      961 gaccggacag gggaatcccg ccctggactg gacctacggc gcccacggga tcgtggccga
     1021 ggtcgacccg gagaccgggg agtacgaggt cctcaagatc gcctcggtct tcgacgtggg
     1081 caaggccatc aacccccaga tcgtccgggg ccagtccatc gggggcatgg tccagggcct
     1141 gggcaccgcg gtctgcgagg gctacatcta cgacgagaag gggatcctcc tcaacccctc
     1201 cttcacggac aacaagatcc cgacggccaa ggatatcccc gcggtcatcg agagcgccgt
     1261 ggtggagacc ccccagatcg acggtcccta cggggcccgg ggggtggggg agcactccat
     1321 gatttccgtg gccgcggccc tgggcaacgc catccaggag gccacggggg ccgagatcgt
     1381 ccatatgccc ctgcgcttcg aggacgtctg gagggccctg cgggacaaga ctcccatcga
     1441 caactggatc accaagagcc ccctgggctc ctgccgttcc gccccggaac tccggaaccg
     1501 gaaaaaggac ggctagggac ctcgttcgcg agcttgcatg tgtctgattc gaccggtgga
     1561 tctcccggcg cgcgcctgcc gggtggggat atggatcggc cgtaggccga aataggttcg
     1621 gccggaaggc cggagcggga tcggccgttg ggccgagcgg aaagagcgat cgaaacaagg
     1681 agcgttccgt ggaaaaaagc ctgaccgtaa agaacaccgt gctgggcatc cagcacacct
     1741 tcgtgatgtt cggggccacc gtgctggtgc ccatcatcac cggcctggac atcggagtca
     1801 ccctgttcgc cgcggggctg ggaaccctgc tcttccacgt actgaccggg ttcaaggtac
     1861 cggttttcct gggcagttcc ttcgccttca tccccggcct cctggccgtg ggcaagagcc
     1921 agggccttcc ctacgccctg ggcggcatcg tcatcgcggg cctcctgtac gtcgtggtgg
     1981 ccatcatctt caagttcgtg ccctacgaca cgatccacaa gatcctgccg ccccacgtca
     2041 cgggccccat gatcatcctg atcggcctca tcctggcgcc ggtggccatc cagaacgccc
     2101 ggggcgactt ctccaagccc atcgtcgacg ccatcggcgt gaacggttgc tgggccgtgg
     2161 ccctcttcac cttcgcggtg gggatcttcg tgaagatcta cctgcccaag ttcggcatga
     2221 agttcgcctc gaacctcccg gtcctcatcg ccctggtcgc ggggtacgtc ctgtccatca
     2281 tcctgggcat cgttgacttc tccgcggtgg ccaaggcgga ctggatcggc attcccaagt
     2341 tcaccctgcc caagttctcc gccgagggga tcagcatcat gctgcccata gccatcgtca
     2401 cgatggtgga gcacttcggg gacatcctgg ccatcggcaa cgtggtgggc aaggacttta
     2461 tcaaggatcc ggggatcagc cggaccctgc tcggcgacgg actcgcgacc tccctggccg
     2521 cgatgatcgg tggaccggcc aacacgacct attccgagaa caccggagcc gtggccctga
     2581 cgggcgccta caatcccatc atcatgcgga tcgccgcggt ctgcgccatc atcctggcca
     2641 tgatcccgaa gttcaccgcc atcatcggca ccatccccgg cccggtcatc ggcgggatct
     2701 ccatcctcct gttcggcatg atctcctcca tcggggtcaa gaacatggtg gacgcccggg
     2761 tcaacctggg gaacgccaag ctcctcatca tcaccgcggc catgctggtc ctgggcctcg
     2821 gcggcgccac cttcgaactc ggcgccctga agctctccgg cctgggcctg gcggcgatct
     2881 tcggcatcgt cctcaacctg atcctccggc cgaaggacat cacctcggcg gagtgcgact
     2941 gatacgatga atccccgggc cctcgcgggg cccctggtgc cccgcgaagc ctgggaagcc
     3001 cgggccgagg gggtgttccg gggggcgatc gccctggcgc accccgacgg actctgggtg
     3061 acgatcctca ggtcccggga ggacatggaa ccccggggca tctggcccgg ccccgaggcg
     3121 ttcgacgcct tggccgggct tgtcgttccg gggcggccgg tcgtattctc gggggaggcg
     3181 gacccgttcc tggcggcccc cgggggaact ctccggaccg gaccgctgga gtcctgggat
     3241 ccccggccgg agctggccga cgccgtggcc gccttccggg aggccggtcc gtccgccccc
     3301 tccgccgcgg cggagctgat gcgggccgcc ctcgcgacgg acgggtatac cgagggccta
     3361 cacggggaag gacccttcgg acgtcggttc gcggagctcc ggaaggagcc gggttttccc
     3421 tgcaacctag cgggcttcgg ccccggcacc accccggcgg gggacgattt cctggcgggg
     3481 tggtgcctgg gcgggatcct taggggggag ggcgaatcgg tccttctcag atttgccggg
     3541 atcgacgggt cgaggaccac ggtccccggc cggaccctcc tggccggggc ggcgcggggc
     3601 ctgttcccgg cctacctggt ccggctggcg tcggccctgg cggccgcggt atccggaggc
     3661 gccggcccgg cccgggaggg cgatcgggac ggtccggacg cccgagggag acgcgaagac
     3721 atactcgcct cggccgcccg ggccgccttc tcccacgggg cgagctccgg gcgggatgcg
     3781 atgttgggat tgatggaagg agttggagga tgaaacttct cagggtccgc aaggacagct
     3841 accacgactc ggtgttcctg atgctcgcca cccaggagct caaggcttcg ggcggggtgc
     3901 tcgatgccgt ggtcgccatg ggtacggaca tgaaccggga actcctgtcg gacatggggt
     3961 tctcgggtcc ggacctggac ggggccgggc cgaacgacct cgtggtagcc gtggaggccg
     4021 gggacgccgc caaggccgag gcggccgcgg cccgggccct ggagctggtg gaccgcaaga
     4081 agtccgcggc cgcggcttcg ggcggaacgg gattggcgcg ctcgggaagc ctggacgcgg
     4141 ccctggggag gcttccgggc gccaacctcg ccgtgatctc ccttcccggg gcctacgcag
     4201 cccgggaggc ccgcaaggcc ctggaccggg gactccacgt gatgctcttt tcggacaacg
     4261 tgcccctgga agaggagatc gccctgaagc gacacgcctc ggccaagggc ctcctggtca
     4321 tggggcccga ctgcgggacc gcgatcgtga acggccagcc cctgtgcttc gcgaacgtgg
     4381 tccgccgggg gaccgtgggc gtggcggccg cctccgggac ggggctccag gaggtgacct
     4441 gcctggtgga ccggtacggg gcggggatct cccaggccgt cggcacgggc gggcgggacc
     4501 tgaagaacgc cgccgtgggc ggccgtacca tgctcctggc cgtcgcggcc ctggccgcgg
     4561 accccgccac ctcggtcatc gtcgtgatca gcaagccgcc ggctcccgat acggccttgg
     4621 ccgtgatgga ggccctgagc cggtcgggga aaccctgcgt cgtccacctc ctgggactca
     4681 aggaccccgc ggtcctcggc aagccggccg ccaacgtcca cctcgcctcg gacctggagg
     4741 actgcgcccg acgggcggcg gccctggccc ggggcgaacc ccatcgcccc gtcgagtggg
     4801 acctatcgga ggccgagatc gagcggatcg tcgagcggga ggcctcgggc ttcgccccgg
     4861 cccagaggca cctccggggc ctctacaccg gcggaaccct ggccgacgag gccctcttcc
     4921 tcctccagcc cctcctgggg ccggtctggt cgaacaacca gaccgatccc gccctggtac
     4981 ccccggatcc ccaggtctcc acgggacaca ccatcgtgga cctgggggac gacgtcttca
     5041 ccgtcggtcg tccccatccc atgatcgacc cctccacccg gacggaccgc ctggacaagg
     5101 aagccgagga tccctcggtg gccgtgctcc tgctggacct ggtcctgggg tacggctccc
     5161 acccggaccc cgcgggcgcc ctggcgcccc gcctggaagc cgcccgggcc aaggccgcag
     5221 cccggggagg acgcctgtcc gtcgtggcct ccgtcaccgg gaccccctcg gacttccagg
     5281 gcctggagga ccagaagcgg aagctcgaag ccgcgggggc cgtggtcatg ccctccaacc
     5341 gccaggccgc gcgcctggcc gcccgaatac tccagaaggg ggccgcccga tgagcgccga
     5401 accgatgaac gtaaaacccg tcctggggct cttctccggg gacctggcgg tcgtcaacct
     5461 gggcctggaa ggcttcgccg agaccctggc cggccgcggg gtgccgacgg tccaggtgga
     5521 ctggaagccc cccgcgggag gccggaagga cgtgatcgaa gccctggacg cgatcgcccg
     5581 gaatccccgg gtggccgagg ccaacgccga agccctgtcc cggatcctgg cgggcaagcc
     5641 gaccctggtc ggtatcggca cggccctgga caccgtcccg ggcatggccc gggacctgat
     5701 cctccacgcg ggccccccgg tggcctggaa ggacatggcc ggcccgctgc ggggcgccgt
     5761 gatcgggggc cttctgtacg agggactggc ctcgaccccc gaggaggccc agaccctggc
     5821 ggcatccggg aaggtccgct tcgagccctg ccaccaccat tccgcggtgg gacccatggc
     5881 gggggtcatg accgcgagca tgcccgtgtg gatcgtggag aaccaggcct tcggcaacag
     5941 ggcctactgc accctgaacg aggggctcgg gaaggtcctc cggtacggag cctattcccg
     6001 ggaggtccta gatcgcctgc gctggatggc cgagcgcctg gcccccgtcc tggccaaggc
     6061 cctggccctg cggggcccga tcgacatgaa gagcctgatc gcccaggtcc tccagatggg
     6121 ggacgagggt cacaaccgca accgcgcggg cacgagcctg gtgatccggg agctcgcccc
     6181 ccacctggcc ctcctggacg agcccaagga ggacatttcc aaggtcttct ccttcctgaa
     6241 cgggaacgac cacttcttcc tgaacctgac catgccgtcg gccaagtgca ccgtggacgc
     6301 ggccctgggg atcgaggcct cctcggtcat cgccaccatg gcccggaacg gcacggagtt
     6361 cggcatccgg gtctccggcc tcggggagcg ttggttcacg ggcccggcga gcgtcgtgga
     6421 gggcctgtac ctcccgggct tctcggcggc ggacgcggcc cgggacgtcg gggactcggt
     6481 gatcacggag acctccggga tcgggggctt tgccatggcc gcggccccgg cgatcgtcaa
     6541 gttcgtgggc gggaccccgg aggacgccct caacgtgacc cgtcggatgt acgagatcac
     6601 cgtgggggag aacgacgcct accggatccc ggccctggac ttccggggca cccccacggg
     6661 gatcgacgtg tgcaaggtgg tggagacggg gatcctcccg gccatcaaca cgggcatcgc
     6721 ccacagggaa cccgggatcg gcatggtcgg cgccggcttg gtcaaacctc ccgcgaactg
     6781 tttcacggac gctgtcttag ccttcgcgga gaaatacggg aactaaaaac gcgggaggtt
     6841 cggcgagcag ggatgcgagc ctggaatacg ccggagcttc gctccggttc gtcaaggacg
     6901 acgcaaagcc cccggaaaac tgtttccggg acgccgtttt ggcgtttgcg gagaagtacg
     6961 ggaggtagca ccgcggaagg tttgcggaac gggcttagac ccgttatagt tcaaccgcaa
     7021 gaaaaatggc cgaacgtccc ggtccgagag ggtccgggac gtcccggcac cgggggccgg
     7081 catgaaaaaa ggtgcgggca agatgatact cctggtcgag gacgaggccc tcatctcggc
     7141 ggcgacgtcc cggaccctgc agaggttcgg gttcaccgtc cggacggccc cgaccggtga
     7201 acgggccgtc gaagccgtcg agactgatcc ggacatcgac ctggtgctca tggacatcga
     7261 cctgggtccc gggatcgacg gcgccgaggc ggcccggcgt atccttcgca tccgggacct
     7321 ccccctggtg ttccactccg cccacacgga gcccgaggtc gtggagaaga ccgaggacat
     7381 cacctcctac ggctacatcg tcaagaactc cggggacacg gtcctggggg cttccatccg
     7441 catggccttc cgcctctggg agagcgaggc caagttccgg accgccttcg agagcgtggc
     7501 ggtcggcatg gtcctcacgt ccccggacgg gaagctcctg cgggtgaacc aggaattctg
     7561 ctccatgctc gggtacactc gggaggaact ggcgtccctg gacttcgccg ccctgaccca
     7621 tccggacgac gtggagacca gtcgtgcccg gatgacggcc ctgcggaacg gggaatccga
     7681 ccggacgcgg tttaagaagc ggtacgtcca caaggacggc cgggagatct gggcggatct
     7741 gagcgtcgtc cttttacggg attccgccgg caagcccctg catttcgtca cccatattca
     7801 ggacatcacg gaggatagac ggatccggga ggaactcgag cgcagccggt cccggttcga
     7861 cctgctactc cgatccgtgc cggcttccat tctcgtagtg caggacggcc gttatgtgtt
     7921 cgcgaatcct tacggagcgc gcctgctggg ctacgaaagt ccggagaacc tggtcggcac
     7981 cttgcagttc gactccatag atgtggagag ccgggaagcc gtaaaactta ggatggcccg
     8041 catagctttg ggcgaatcca atgatccaag ggagttaatc gtacttcgaa aggatgggac
     8101 aagggtcacg acggaatccg cctccctacc catcgagtac gacggccgtc ccgcggccct
     8161 tatcctgggc cgggacaaga cggagcagaa ggcggtggag ggccgcctgg ccagcatcct
     8221 gaaggccgtg cccaccggga tcggggtcgt gcagggaccg gatcgggaga tccgcgaggt
     8281 gaacgacaag ctctgcgaga tgaccgggta ttcccgcgag gagctcgagg gccggagcat
     8341 cgccctgttc tacgcggacc cggcggagta cgagcggatc ggccgggaaa agtaccggca
     8401 gatggaggaa cggggatacg ggaccgcgga gacggtctgg cggcacaagg acgggcggac
     8461 cctggatgtc ttcctggcct ccgcccccct gtatcccgga gacctgtccc ggggggtgac
     8521 cttcgccgcc ctggacatca cggagcgcaa gcgggcggag gaggcggtcc gcagcctcct
     8581 tcaggaaaag gatctcctcc tccgggagac ccaccaccga gtcaagaaca acctgggact
     8641 cgtccacagc cttctttccc tgcaggcggg gcgtcaggcg gatccggccg cccggggcgt
     8701 cctgggggac gcggccgtcc gggtccaggc catgtcccgg ctctacgacc gtctgtaccg
     8761 gtccgagaac ttccgggaga tgagcctcaa gtccttcctg cccggcatcg tggaccaggt
     8821 acgggacttg ttccgactag acgcggaggt acgggcggac ctgcggatcg aggacatccc
     8881 ccttcccgtc cgcaccctca ctcccctggg catcatcctg aacgagctgg tcacgaattc
     8941 gatgaagtac gccttccagg gacgaccgga gggtcggatc accctgacgg ccctccggga
     9001 cggcccggtc gtgcggatca cctacgggga cgacggcccg ggtctgccgg agtccttcaa
     9061 tctcgacacc cccgcgggct tcgggcttca gctctgcggc atcctttccg gacagctcgg
     9121 aggaaccctg agactggaac gggaccgagg tacccgcttt gtcctggagt tcccggcgaa
     9181 ctaggggagc gggaccgcgc aaggtttcgg gaggtcattc ggttttcggc cgtgcctccc
     9241 tgccgggacc ccgtaccagc cccaggaata cgaccagggc ggctccctgc acggccacgg
     9301 agaaggcgac caagggaacc gccccgaagc ggtcgtagag caggccgatc accgcgctcc
     9361 ccgcgaacca ggccagtccg aagagggtgt tgaaaagccc gaaggcccgg gccctccggt
     9421 cctcggggac ccggtccccg atggcggcct tgagtatgga ctcctgggtt cccacgctga
     9481 tgccccagag ggccatgccg accaggatgg ccgccgggcc tcccaggaag accagggggg
     9541 cggtgaagag ctccgcggcg aagaggaccg ccaggacctt gagccccgcc ttgtcgtacc
     9601 accgcccgaa caccagggcc gccgcggcgt ccacggccat ggccccggcg tagagcaggg
     9661 gaatggcctc ctcggggaag ccggcgccgc gtttcaggtg caggccgatc agggggaagt
     9721 cggttattcc catggccagg aggcccgcgg ccaggacgat gatccagtag ctccgggagt
     9781 agccggaagt cccgacctcc acggcgctct tcttgagctc gaaccgttcc ggatggggga
     9841 agaggaagcg gttgtagacc gtgaaaccta ccgccagagc cgccgggatg aagaggagca
     9901 ggaggcccgt ccggtatccc gccagggtct tggcgtcggg gcccaggaag aggagggccg
     9961 agaagagggc gggtcccagg aaggccccga tctggtccat ggcctcgtgg agcccgaatc
    10021 cgaaaccgct gccggtttgc ttggccgcga aggacaccac cgcgtcgtgg gcgggcttgc
    10081 ggatcgcctt tcccacccgc tccaggaaga ggaagaccac cgccagttcc cagccccgca
    10141 cgaaggccag catgggcagg gagcagagct ggaccacgta gcccaggatg atgagggtcc
    10201 agtatttccg ggtccggtcc gcgatgctcc cggacaccag gcgcagggcg tagcccacga
    10261 actccccgag ccccgcggac agccccaggg cgaaggcgct ggtgcccaac agcttgaggt
    10321 actggcccac cacgctccgg cccccctcgt agttcatgtc cgcgaagagg ctgacgatcc
    10381 cgaacaggac gatcagggct acggcggcct tccgggtgag ctcgacgggc ttacggtcca
    10441 tacggcgctc cttgggacga agttaaggga gcatatattg agctcccttc cgacgcaaga
    10501 accgccccgt cgttggacat tcggatcgaa agtgtcaaga tccccttgcc tatcccccgg
    10561 atcggtgaga aaatcccctg tccgcccggg ttgcgcgcgg gcgctttttg tcggattata
    10621 gggggaagcg agccgcggtc ccccgcagga cccttccagg aggttttcca tgaagatgat
    10681 cgacctgacc attcccctcg gcatcggcac cccgccctgg cc
//
LOCUS       DAOTEI010000122        10652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4382_length_10652_cov_4.968199, whole genome shotgun sequence.
ACCESSION   DAOTEI010000122 DAOTEI010000000
VERSION     DAOTEI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4382_length_10652_cov_4.968199"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="P5313_11600"
     CDS             complement(<1..656)
                     /locus_tag="P5313_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRY81052.1"
                     /translation="MKDRIIGRVRRVNGPVIEADGITDAQMLELVRVGESRLVGEVLK
                     LSGDRAVIQVYEDTTGLAPRDNVYGAGMPLSLELGPGLIGSIYDGIQRPLEALRSLSG
                     HYIESGITAEPLDRTRTWRFVPDPDLKPGAPVAGGRILGTVRETSRVEHRVLVPPEVS
                     GTLESLVPEGDYPVDRRIAVVRTDSGTRELPMLQSWPIRIPRPVRQRRPLSIPLITGQ
                     R"
     gene            complement(666..1307)
                     /locus_tag="P5313_11605"
     CDS             complement(666..1307)
                     /locus_tag="P5313_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HRY81053.1"
                     /translation="MEAERQEDRDLLSGIEREAQLKAEELAQEAAAYARNRSEAARAQ
                     TREILRKAEEIAEAQARAVLRNAESRATVESRKAFLRGRDGLIQETLEAARILAARAA
                     ETPGYEDVLRGWIVEAAVGLSEPEARVNASREELPRITDELLHRAEREVLELTGKPVR
                     LSKVEGDPLPEQGVVLTAEGGRLAYNNQVPVRFLRAQSEIRKQIHAVLFGEGA"
     gene            complement(1318..1623)
                     /locus_tag="P5313_11610"
     CDS             complement(1318..1623)
                     /locus_tag="P5313_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRY81054.1"
                     /translation="MKYYVIGDEDAVLGFGLAGVRGRRVRTSEEASAAFREALEDREN
                     AIILVTERIAELIRPQVDRYVFKEDFPLIVEIPDWEGKLSSRPPLRETVNAAIGIKL"
     gene            complement(1631..1999)
                     /locus_tag="P5313_11615"
     CDS             complement(1631..1999)
                     /locus_tag="P5313_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRY81055.1"
                     /translation="MQDPKKLFAGRVRLSVVVLMAFLGIFGLVSVGGASAQTPESRTP
                     QVTREQADIQKFGLIAAAVAFGFGAIGAGIAIANVGAAAMGAIGEKPEIASQALIFIA
                     LAEGLVVFGFITALMILGKF"
     gene            complement(2014..4065)
                     /locus_tag="P5313_11620"
     CDS             complement(2014..4065)
                     /locus_tag="P5313_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRY81056.1"
                     /translation="MIFTTPMRLLAAVVLDRSSEEVSGELLRLGQLEFISVRDLTAEW
                     KDRVAPVPSDGTETRIEELRRRLEGFLRMPGTPVRLPVPSLEDRTPLDLAAAEKALDA
                     LAAEVGAIRDRQKAVQEDVLRLSEMRRQLEVFDDLSKPSGLGSAYSFLAVRTGMVPSS
                     RFPALEEELAALPSVLVPWGDPAAPSRSALAVTLKKDEGRAAPILARAGWEDGILSGE
                     PGEETSRVTPSRDGKARALQGIDARIAGLRERQGMYQAELEDLFRRKGPDLESMWARL
                     RVRELLGRVRSRFARTERTVLFSGWIPGDAAAAVEEGIRKAAGGRCHVEWISEKEGRE
                     HNQEAPVALKNPRVLRPFEALVRNYAVPEYGSVNPVPFTALAYLVMFGLMFGDAGHGL
                     VLALLGGAGVLAARRSGKDDTLARLILYCGCSAIVAGVLFGSYFGMPWLPPVWFDYHG
                     VVTGREAGAGGIRSVYDILGITIKFGLAVLGTGLVLNWINLVRRRRWLTLVLDKGGLA
                     GGWIYAAGTWAAFRFVGSGYRTLPDPDLLFWILGLPTLALALKGPAEYLLHPREHRKP
                     FGALTIVDFFMEWIVEVLEIYSGYLANTLSFMRVAGLGIAHVSLMTAFFQIARMVNPR
                     GGFSAAAAAVLVLGNVLVIALEGLSAGIQSLRLNYYEFFSKYFNGTGRAFRPISLGTK
                     E"
     gene            complement(4062..5126)
                     /locus_tag="P5313_11625"
     CDS             complement(4062..5126)
                     /locus_tag="P5313_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRY81057.1"
                     /translation="MPGALAVYGFLNAKLKARISGLLSPEALETLVRAPTLSEAMVLL
                     KDTPYSALEAEYSRTGDLRGCEALLYSMEVEYHSGLERFAKEPTRSVIRAHAVRFEAE
                     TLKNVVRLWFETRVRGRPSDGSGVYLYRGPLPHRLDLDALRTAPDSAALADAVSGTPY
                     EAVLRRTAPRAESEGTLFPLESELDRLAFSVLLASLDGLSRRDRDIARRVLGIEIDLH
                     NVQWLARAQHFYRLGPEEALASLVPGGSSYDAAKAASAYRSGNVSELTQALLGGRNRG
                     LAALAGTGGDPSARLAFLERALRTVLRGESGRLLAGHPFTIGTVLAYFALRREETRTL
                     LAILNAKYYALPEERIRNSL"
     gene            complement(5131..5445)
                     /locus_tag="P5313_11630"
     CDS             complement(5131..5445)
                     /locus_tag="P5313_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81058.1"
                     /translation="MDRVLEEENKARTLLEEARKRADAERARADAEAEAVLSEARARA
                     AALLKERLDRARTEARDLETRALEEAERRSREALQEARTRIPDLARRIADRILSSDLG
                     NG"
     gene            5635..6270
                     /gene="pncA"
                     /locus_tag="P5313_11635"
     CDS             5635..6270
                     /gene="pncA"
                     /locus_tag="P5313_11635"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HRY81059.1"
                     /translation="MIRPSPRSAFIAVDLQNDFCPGGALGVPDGDAVLPVVNRVVRSF
                     PRAVATQDWHPRGHFSFASSRPGAKLYSTVDAEGISQILWPDHCVQGTPGAAFHPGLD
                     LEPYSLIIRKGFRPDLDSYSTFFENDRKTPTGLHGWLHDLGVKEVWLAGLATDFCVLY
                     SALDAVCLGYTAYVLTDAVRAVNVPEGSGEQALKSMAAAGVRLTTSSEIPG"
     gene            6267..7694
                     /locus_tag="P5313_11640"
     CDS             6267..7694
                     /locus_tag="P5313_11640"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRY81060.1"
                     /translation="MISAAFTDLYELTMAQGYLVHGMDRPAVFEAFFRHHPFGGGYSV
                     FAGLGPLLEDLENLRFLPEDLAYLEGRGIFSADFLSFLEAFRFRGDLWSVPEGEVVFP
                     REPLIRVRGGLVECQLVEGLLLNRVNFQSLVATKTARVCHAAKGGRIMEFGLRRAQGP
                     DGANSATRASYVGGAVGTSNVLGARLFGIPAMGTMAHSWIMAFPSEREAFEAYADLYP
                     DATVFLVDTYDTLASGVPNALAAGRRLAERGKRFGIRLDSGDIQYLAEESRKMLDAAG
                     FRDSFIVASNDLDEEIIEALTAAGVPVDIWGVGTRLVTGGSDSAFSGVYKLAAVDRGG
                     GMESAMKFSDNPDKSTEPGVKEVYRIYDEAGQARADVLAAEGETLEPGVPRTFHHPAL
                     DTRRFRMTPCGEIRPLLAKAMEGGKRLGPPPPLAEARDRCKTELGRFDPTYRRLLNPH
                     VYKVSITDAVRDLKASFLERYRAGT"
     gene            complement(7708..9165)
                     /locus_tag="P5313_11645"
     CDS             complement(7708..9165)
                     /locus_tag="P5313_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81061.1"
                     /translation="MADFAVRLFLLLLPVALLLLAGLRLRRRAVFPHGYFPSARSRAS
                     EVLLRSLRLRYDLILDALVAILVALALSGWPSPGPGRTAAVLDGSRSMFRGEPGSRAV
                     DRAVSTLYEREDLAGARVFLLDFDPDRGRYRLRDFTRHRREEGPEGFAARAAREAEAL
                     GVDWGILASLRSRGYGRIVVFTDGLPGDPVEFEAVETGFSELPYAYPASAGYEEATGS
                     WALRLAVEGEPSGLIVHRWEEGPATFVRLSTDSLDLAEGPSGWILRFPKPGLVVVAVG
                     GETFPVLLPPRPARPDGSGDFSKAMASVFSFLPDRPGEGLGFLDSGRRRPGRSFVTSL
                     GSSDTPLVADPSEAFGGLVPLAEPDARAGRADIALGRGSLDSSDLPLLYWNRLQSSIP
                     APYFSGPERAGSGWKPQGSGYWRLGRAGPEALLPPIGEYAQSARTGSLRIPEREYRRW
                     PIAAALAALFALKLALKRYFSGASKIESPAARGGP"
     gene            complement(9158..10000)
                     /locus_tag="P5313_11650"
     CDS             complement(9158..10000)
                     /locus_tag="P5313_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY81062.1"
                     /translation="MISVDPECPALAAAFRRRNLRAHLPRGVAGRDPMFRRSGSSFDL
                     KTIREYAPTDDPRSIDWRLLGRTDRTFVREYYEEETEGVAFLVDLSGSLSAADRDSYG
                     SLVLSLGYLCLELGLSLSAHGFSDRPDAAGLRLRGRSGVSELASWLRSAEFRGPTDIS
                     RAVRTLRGLSSHRRLFLFSDFQDPGFDPRSVGFPSACLFHLRRGFEEVAEGWGEYEVL
                     DPEGGRRILVPWDPATWDAYRRAEEARAESLGRPRKGFRYFRLSAGDDRAPVYWKVLE
                     YLYG"
     gene            complement(10005..>10652)
                     /locus_tag="P5313_11655"
     CDS             complement(10005..>10652)
                     /locus_tag="P5313_11655"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY81063.1"
                     /translation="INRATPKTQSAFLEAMEERRITFLGETYPLPDPFFVIATQNPIE
                     LEGTYPLPEAQVDRFFMKLRFSPPDFRALKDILRISERPPEGSPAGREQTGEVLALWA
                     QASREMAAAEEVKNFIAKLVLATHPDHSPVEEVRKYVLYGASPRCAISLLRAAKAKAA
                     LAGRAAVSYDDVGELFEDVSAHRVILNFDAEADGVRKRDILTAARNAVRREFFER"
BASE COUNT         1637 a         3730 c         3721 g         1564 t
ORIGIN      
        1 cgctggcccg tgatgagggg gatcgagagg gggcgtcgtt ggcgtacggg gcgcgggatc
       61 cggatgggcc agctctggag catggggagt tcccgggttc ccgaatccgt gcgtaccacg
      121 gcgatcctcc ggtccacggg gtagtccccc tcggggacca gggactccag ggtgccggag
      181 acctccggcg gcaccaggac ccggtgctcc acccgggagg tctcccggac ggtgcccagg
      241 atccggccgc ccgcgaccgg ggcgccgggc ttgaggtccg gatccgggac gaagcgccaa
      301 gtccgggtcc ggtccagggg ctccgcggtg atcccgctct cgatgtagtg gccggacagg
      361 gagcgcaggg cttccagggg ccgctggatt ccgtcgtaga tggagccgat gaggcccggg
      421 cccagctcca aggagagggg catccccgcc ccgtagacgt tgtcccgggg ggccaggccc
      481 gtggtgtcct cgtagacctg gatcaccgcc cggtcccccg agagcttcag cacctcgccc
      541 accaggcggg attccccgac ccggaccagc tccagcatct gggcgtccgt gattccgtcg
      601 gcctcgatca ccggtccgtt cacccgccgg acccggccga ttattcgatc cttcatagac
      661 ctgccttacg cgccttcccc gaagaggacg gcgtgaatct gtttccgtat ctccgactgg
      721 gcccggagga agcggacggg cacctggttg ttgtaggcca gccttccgcc ctccgcggtc
      781 aacaccaccc cctgctcggg caggggatcc ccctccacct tggaaagccg gaccggcttc
      841 ccggtcagtt ccaggacctc ccgctccgcc cggtgcagga gctcgtccgt gatccggggc
      901 agctcctccc gggaggcgtt gacccgggcc tccggctcgg acaggccgac ggcggcctcc
      961 acgatccagc cccggaggac gtcctcgtat ccgggagtct ccgcggctcg ggccgcgagg
     1021 atccgggcgg cctccagggt ttcctggatg aggccgtccc ggccccgcag gaaggccttg
     1081 cgggactcca cggtagcccg ggattcggcg ttgcgcagga cggcccgggc ctgggcttcc
     1141 gcgatttctt cggccttcct caaaatctcc cgggtctggg cccgggccgc ctcggaccgg
     1201 tttcgggcgt aggccgccgc ctcctgggcg agctcctcgg ccttgagctg ggcctcccgc
     1261 tcgatgcccg aaaggaggtc ccggtcttcc tgtcgttccg cttccatgcc tagcgcctca
     1321 cagcttgatc ccgatggccg cgttcacagt ctcccgcagg ggcgggcggg agctgagctt
     1381 gccctcccag tcggggatct ccacgatcag ggggaagtct tccttgaaga cgtagcggtc
     1441 cacctgggga cggatcagct ccgcgatccg ctcggtgacg aggatgatgg cgttttcccg
     1501 gtcctcgagg gcctcccgga aggccgcgga ggcctcctcg gaggtccgga cccggcggcc
     1561 gcggacgccg gccaggccga agcccagcac cgcgtcctcg tccccgatca cgtagtactt
     1621 catccggggc ctagaatttg cccaggatca tgagggccgt gatgaagccg aacaccacca
     1681 ggccttccgc cagggcgatg aagatgaggg cctggctggc gatctcgggc ttttccccga
     1741 tggcccccat ggccgccgcg cctacgttgg ctatggcgat gccggccccg atggccccga
     1801 acccgaaggc cacggccgcg gcgatgagcc cgaatttctg gatgtccgcc tgctcccggg
     1861 taacctgggg ggtacgggac tccggggttt gggcgctcgc cccgcccacg gacaccaggc
     1921 cgaagatgcc caggaaggcc atcaagacga cgacgctcag gcgcacccgg ccggcgaaca
     1981 gcttctttgg atcctgcatg gaaaactcct tgttcattct ttcgtcccca gggagatcgg
     2041 ccggaaggcc cggccggtcc cgttgaagta cttggagaaa aactcgtaat agttgagcct
     2101 cagggactgg atccccgcgg acaggccctc cagtgctatg accaggacat tgccgaggac
     2161 caggaccgcc gccgcggccg cggaaaagcc tccccggggg ttcaccatcc gggctatctg
     2221 gaagaaggcc gtcatgaggc tcacgtgtgc gattcccagg cccgccaccc gcatgaacga
     2281 aagggtgttg gccaggtacc ccgaatagat ctccaggacc tcgacgatcc actccatgaa
     2341 gaagtccacg atcgtcaggg ccccgaaggg tttccgatgc tcccgcgggt ggaggaggta
     2401 ttccgccggc cccttcaacg ccagggcgag ggtcgggagg cccaggatcc agaagaggag
     2461 gtccgggtcg gggagggtcc gatacccgct cccgacgaag cggaaggccg cccaggtccc
     2521 ggccgcgtag atccagcccc ccgccagacc gcccttgtcc aggaccaggg tcagccagcg
     2581 cctcctgcgg accaggttga tccagttcag gaccagcccc gtgcccagga ccgccaggcc
     2641 gaacttgatg gtgatcccca ggatgtcgta gaccgagcgg atgccgccgg ctccggcttc
     2701 gcggccggtc accaccccgt ggtagtcgaa ccagaccggg ggcagccagg gcatcccgaa
     2761 gtaggaaccg aagagaaccc ccgcgacgat cgcggagcac ccgcagtaca ggatcagccg
     2821 ggctagggtg tcgtccttgc ccgaacgtcg ggcggccagg accccggcgc ctcccaggag
     2881 ggccagcacc aggccgtgcc ccgcgtcccc gaacatgagc ccgaacatca ccaggtaggc
     2941 cagggccgtg aagggcacgg gattcacgga gccgtactcc gggaccgcgt agttccggac
     3001 cagggcctcg aaggggcgga ggacgcgggg attcttcagg gccacggggg cttcctggtt
     3061 gtgttcccgg ccctccttct cggagatcca ttccacgtgg cagcggccgc cggccgcctt
     3121 ccggatcccc tcctccacgg cggccgccgc gtccccgggg atccagccgg agaagagcac
     3181 cgtccgctcg gtgcgggcga accgggacct cacccgtccc aggagctccc gtacccggag
     3241 ccgggcccac atcgattcca ggtccggccc cttcctgcgg aagaggtcct ccagttccgc
     3301 ctggtacatt ccctggcgct ccctaagccc ggctatccgg gcgtcgatcc cctggagggc
     3361 ccgggccttg ccgtcccggg agggtgtaac gcgagacgtc tcttcgccgg gctccccgga
     3421 gaggatgccg tcctcccagc ccgcccgggc caggatgggc gccgcacgcc cctcgtcctt
     3481 tttcagggtg accgccaggg cgctccggct cggggcggcc gggtcccccc agggcacgag
     3541 gaccgagggg agggcggcca gctcctcctc cagggccggg aaccgggagg acggcaccat
     3601 cccggtacgg acggccagga aggagtaggc ggaaccgaga cccgagggct tggacaggtc
     3661 gtcgaagacc tccagctggc gccgcatctc cgagagccgc aggacatcct cctggacggc
     3721 cttctggcgg tcccggatgg cccccacctc cgcggccagg gcgtccaggg ccttctccgc
     3781 cgcggccaag tccagggggg tccggtcctc cagggaggga accggcaggc ggaccggggt
     3841 tcccggcatg cggaggaagc cctccagcct gcggcggagc tcctcgatcc gcgtctccgt
     3901 cccgtcggag ggaacggggg ccacccggtc cttccactcc gccgtcaggt cccggacgga
     3961 gatgaattcc agctgtccca gccgcaggag ctccccggag acctcctcgg agctcctatc
     4021 caggactacg gccgcgagga gccgcatggg ggtcgtgaag atcataggga gttcctgatc
     4081 ctttcctcgg gaagggcgta atacttggcg ttgaggattg ccaggagggt ccgggtctcc
     4141 tcccgccgga gggcgaagta ggccaggacc gtgccgatgg tgaagggatg gcccgccagg
     4201 agccgtccgg attcgccccg caggacggtc cggagggccc gttccaggaa ggcgagccgg
     4261 gcggaggggt cccccccggt accggccagg gcggccaggc cccggttccg tcctcccagg
     4321 agggcctggg tcaattcgga gacgttcccg gaccggtacg ccgatgcggc cttggcggcg
     4381 tcgtaggaac ttccgcccgg gaccagggag gccagggctt cctccgggcc caggcggtag
     4441 aaatgctggg cccgagccag ccactggacg ttgtggaggt cgatctcgat ccccaggacc
     4501 cttcgggcga tgtcccggtc ccgtcgggac agtccgtcca gggaggccag gaggacggag
     4561 aaggccagcc ggtccagttc cgattccagg gggaagaggg ttccttccga ctccgcccgg
     4621 ggagccgtcc ggcgcaggac cgcctcgtag ggggttccgg acacggcgtc ggccagggcg
     4681 gccgagtccg gggccgtacg cagggcgtcc aggtcgagcc ggtggggcag gggaccccgg
     4741 tacagatata cccccgagcc gtcggagggg cgcccgcgta cccgggtttc gaaccagagt
     4801 cggaccacgt tcttgagggt ctccgcctcg aaccggaccg cgtgcgcccg gatgacggag
     4861 cgggtgggct ccttggcaaa acgttccagg ccggagtggt attccacctc catcgaatac
     4921 aggagggcct cgcagccccg gaggtcgccg gtgcgggagt attccgcctc cagggcggag
     4981 tagggagtgt ccttcagcag gaccatggct tccgagaggg tcggggcccg gacgagggtt
     5041 tccagggctt ccggggaaag tagtccgctt atcctcgcct tgagcttggc gttcaggaaa
     5101 ccgtagacgg ccagggcacc gggcataccg tcagccgttc cccaggtcgg aggacaggat
     5161 gcggtcggcg atccggcgcg ccaggtcagg aatgcgggtc cgggcctcct ggagggcttc
     5221 ccggctccgg cgctcggcct cctcgagggc gcgggtttcc aggtcccggg cttccgtccg
     5281 ggcccggtcg agccgctcct tgaggagagc cgccgcgcgg gccctggcct cggataaaac
     5341 cgcctcggcc tccgcgtccg cccgggcccg ctcggcatcc gcccgctttc gggcttcctc
     5401 gaggagggtg cgggccttgt tctcttcttc cagaacgcga tccacgatgt taagcatacg
     5461 ctcgccttcg gccggccggg tacgcggcgg gcacggggaa cgcggcgctc gaacggggcg
     5521 tcggggggtt ccgaggcgga aggctatacc tccggaaaaa aaatgtacag acccccctcg
     5581 attcgaacct ttacgcgccc gggtctccgg ggtatccttg tcccggaggt gcccatgatc
     5641 cgtccatccc cccgatccgc cttcatcgcc gtggacctgc agaacgactt ctgtcccgga
     5701 ggagccctgg gggttccgga cggggacgcc gtcctgcccg tcgtcaaccg cgtcgtccgg
     5761 tccttccccc gggccgtggc cacccaggac tggcaccccc ggggacactt ttccttcgct
     5821 tcctcccgcc ccggcgcgaa actgtattcc accgtggatg ccgagggcat ctcccagatc
     5881 ctctggcccg accactgcgt tcagggaacc ccgggggcag ccttccaccc gggactggac
     5941 ctggagccct acagcctgat catacgcaag ggattccgcc ccgacctgga ctcgtactcc
     6001 accttcttcg aaaacgaccg caagaccccc acgggccttc acggctggct ccacgacctg
     6061 ggggtcaagg aggtctggct ggccggcctg gccacggact tctgcgtcct gtattccgcc
     6121 ctggacgccg tctgcctggg ctatacggcc tacgtcctga ccgacgcggt ccgggcggtg
     6181 aacgttcccg aagggtccgg ggagcaggcc ctcaagtcca tggccgccgc gggcgtccga
     6241 ctcacgacga gttccgagat ccccggatga tcagcgccgc cttcacggac ctctacgagc
     6301 tcacgatggc ccagggctac ctcgtccacg gcatggaccg gccggcggtc ttcgaggcct
     6361 tcttccgcca ccatcccttc ggcgggggct attccgtgtt cgcgggcctg gggcccctcc
     6421 tcgaggacct ggagaacctt cggttccttc cggaagacct ggcctacctg gagggtcggg
     6481 ggatcttttc cgccgacttc ctttccttcc tggaagcctt ccgcttccgc ggcgacctct
     6541 ggtccgtgcc ggagggggag gtggtgttcc cccgggagcc cctgatccgg gtccggggcg
     6601 ggctggtgga gtgccagctc gtggaagggc tgctgctcaa ccgcgtcaac ttccagagcc
     6661 tggtggccac gaagaccgcc cgggtctgcc acgcggccaa gggcggcagg atcatggagt
     6721 tcggcctgcg ccgggcccag gggccggacg gggccaattc ggccacccgg gcttcctacg
     6781 tcggcggggc cgtgggcacc tccaacgtcc tgggcgcccg gctcttcggg atcccggcca
     6841 tggggaccat ggcccattcc tggatcatgg ccttcccgtc ggagcgggag gccttcgagg
     6901 cctacgcgga tctgtacccg gacgccaccg tcttcctggt ggatacctac gataccctgg
     6961 ccagcggggt tcctaacgcc ctggcggcgg gccgccgtct ggcggagcgg ggcaagcgtt
     7021 tcgggatccg cctggattcc ggggacatcc agtacctggc cgaggagtcc cgcaagatgc
     7081 tggacgccgc ggggttccgg gactccttca tcgtggcctc caacgacctg gacgaggaga
     7141 tcatcgaggc cctgaccgcc gcgggcgtcc ccgtggacat ctggggtgtc ggaacccggc
     7201 tggtgaccgg cggctccgac tccgccttct ccggggtcta caagctggcc gccgtggacc
     7261 gcgggggcgg gatggaaagc gccatgaagt tctcggacaa ccccgacaag tccacggaac
     7321 ccggcgtcaa ggaggtctac cggatctacg acgaggcggg gcaggcccgg gcggacgtcc
     7381 tggccgcgga gggggagacc ctggagcccg gggtcccgag gaccttccac cacccggccc
     7441 tggacacccg caggttccgg atgaccccct gcggggagat ccggccccta ctggccaagg
     7501 cgatggaggg cgggaagcgc ctcggaccgc ccccgcccct ggccgaggcc cgggaccggt
     7561 gcaagaccga actcggacgc ttcgatccca cctaccggcg cctcctcaat ccccacgtct
     7621 acaaggtctc catcacggac gccgtccggg acctgaaggc gtccttcctg gagcggtacc
     7681 gggcggggac ctgacggatt gcgggctcta cggcccgccc cgcgcggccg gggattcaat
     7741 tttagaagcc ccggaaaaat acctcttcag ggcgagtttc agggcgaaca gtgcggccag
     7801 ggcggccgcg atcggccagc gacggtactc ccgctccggg atccggaggg agcccgtccg
     7861 ggccgattgg gcatattccc cgatcggcgg caatagggct tccgggccgg cccggccgag
     7921 gcgccagtag cccgagccct ggggtttcca ccccgacccc gcccgttccg gcccggagaa
     7981 gtacggcgcc gggatcgacg actgcaggcg gttccagtac agcaggggca gatccgacga
     8041 atccaggctg cccctgccca gggcgatatc cgcccgtccg gcccgcgcgt ccggttccgc
     8101 caggggaacg aggccgccga acgcctcgga ggggtccgcg accagggggg tatcggagct
     8161 tcccagggag gtcacgaagg atcggcccgg ccggcgccgg ccggagtcga ggaatcccag
     8221 gccttcgccg ggccggtcgg gaaggaagga gaagaccgag gccatggcct tgctgaagtc
     8281 gccggatccg tcgggccgcg cgggccgggg agggaggagg acggggaagg tctccccccc
     8341 gacggcgacc accacgagtc cgggcttggg aaaacggagt atccacccgg aggggccctc
     8401 cgccaggtcc agcgagtccg tggacagccg gacgaaagtc gcgggtccct cctcccaccg
     8461 gtggacgatg aggccggacg gctccccctc cacggccagc cgcagggccc agcttccggt
     8521 agcctcctcg tatcccgcgg aggcggggta cgcgtagggc agttcggaaa agcccgtctc
     8581 cacggcctcg aattcgacgg ggtcgccggg caggccgtcc gtgaacacga cgatcctgcc
     8641 gtacccccgg ctcctgaggg aagccaggat cccccagtcg acccccaggg cctcggcttc
     8701 tcgggccgcc cgggccgcga atccctccgg accctcctcc cgccggtgcc gggtgaagtc
     8761 ccgcaggcgg tatcttcccc ggtccgggtc gaagtccagc aggaagacgc gggctcccgc
     8821 caggtcctcc ctctcataca aggtgctgac tgcccggtcc acagcccggg acccgggttc
     8881 cccgcggaac atggaccggg aaccgtccag caccgccgcg gtcctgcccg ggcccgggga
     8941 aggccagccg gacaaagcca gggccaccag gatcgcgacc agggcgtcca ggatgaggtc
     9001 gtacctcagg cgcaggctcc gcaggaggac ctcggaagcc cgggaccggg cggaggggaa
     9061 gtagccgtgg gggaagacgg ccctgcggcg caggcggagc ccggccagga ggaggagggc
     9121 caccgggagg agcaggagga agagccggac ggcgaaatca gccatacagg tactccagga
     9181 ccttccaata cacgggcgcc cggtcgtccc cggcggacag gcggaagtac cggaagccct
     9241 tccgggggcg gccgagggat tcagcccggg cttcctccgc acgccggtat gcatcccagg
     9301 tcgcgggatc ccaggggacc aggatcctcc ggccaccctc cgggtcgagg acctcgtact
     9361 ccccccagcc ttcggcgacc tcctcgaagc ctcggcggag gtggaagagg caggcggacg
     9421 ggaacccgac gctccgggga tcgaagccgg gatcctggaa gtccgagaac aggaagagcc
     9481 tccggtggga agagagcccc cggagggtcc ggacggcccg ggagatgtcc gtcggtcccc
     9541 ggaattccgc ggacctcagc caggaggcca actccgacac cccgctccgt ccccgaagcc
     9601 gcagtcccgc cgcgtccggc cgatccgaga acccgtgggc gctcagggac agccccagct
     9661 ccaggcagag gtagcccagg gacagcacca ggctcccgta ggaatcccgg tccgccgcgg
     9721 acaggctacc ggacaggtcc accaggaagg cgaccccctc ggtctcctcc tcgtagtatt
     9781 cccggacgaa ggtccggtcc gtccggccca ggagcctcca gtcgatgctc cggggatcgt
     9841 ccgtcggggc gtactcccgg atggtcttca ggtcgaagct ggagcccgag cggcggaaca
     9901 tggggtcccg gccggccact ccccggggaa ggtgggcgcg gaggttccgc ctgcggaagg
     9961 cggccgccag ggcggggcac tcggggtcaa cggagatcac ggccctaccg ctcgaagaat
    10021 tcccggcgca cggcgttccg ggcggccgtc aggatgtccc gcttgcggac gccgtccgcc
    10081 tctgcgtcga agttgaggat gacccggtgg gccgacacgt cctcgaagag ctcccccacg
    10141 tcgtcgtagg acaccgccgc gcggcctgcg agggcggcct tggccttggc ggcccggagc
    10201 agggagatgg cgcagcgggg gctggcgccg taaaggacgt acttccgtac ctcctccaca
    10261 ggggaatggt ccggatgggt cgccagcacg agcttggcga tgaagttctt cacctcctcc
    10321 gccgcggcca tctcccggga ggcctgggcc cagagggcca ggacctcgcc ggtctgttcc
    10381 cgacccgcgg ggcttccctc ggggggtcgc tccgagatcc gcaggatgtc cttgagggcc
    10441 cggaagtcgg gaggggagaa gcggagcttc atgaagaacc ggtccacctg ggcctcggga
    10501 aggggatagg tcccctccag ttcgatggga ttctgggtgg cgatcacgaa gaaggggtcc
    10561 gggagggggt aagtctcgcc caggaaggtg atccgccgct cctccatggc ctccaggaag
    10621 gcggactggg tcttgggggt ggcccggttg at
//
LOCUS       DAOTEI010000123        10500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4452_length_10500_cov_5.305792, whole genome shotgun sequence.
ACCESSION   DAOTEI010000123 DAOTEI010000000
VERSION     DAOTEI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4452_length_10500_cov_5.305792"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="P5313_11660"
     CDS             complement(<1..818)
                     /locus_tag="P5313_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRY81064.1"
                     /translation="MDALASTIRKLLASYEEAGGINHSDGPNLPSKQSVAAIVDDLET
                     LAFPGYRREDGIVDTNADFLIGELVHRTARNLVRELERSLDYRLRMETGRGCPAACHM
                     EARDLAYDFLESLPGIRTVLMEDVAAAFSGDPAAKSVEEVILSYPGMEAIVAHRFAHW
                     FWERRIPLLPRMMSEYLHGKTGIDIHPGARIGRMFFIDHGTGVVIGETAVIGDRVKIY
                     QGVTLGALSVRKAEGDVKRHPTIEDDVTIYSGATILGGQTVIGRGSAIGGNVWLT"
     gene            complement(847..2157)
                     /locus_tag="P5313_11665"
     CDS             complement(847..2157)
                     /locus_tag="P5313_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY81065.1"
                     /translation="MTKVPNSFRPFLGTTFLIGFGFFTMGLMDPLYDTYVPIFLGKYI
                     RSMGIVGSVMTLDNIFALFLIPIVAVWSDKIRSPIGRRMPFILVTLPLTAVCFAWLPY
                     AAAASLAALVAAIFLLNIWKQSARGPVVALMPDIVPGEFRSEANGVINTMGGIASIVG
                     TLGLARLMDLDVTLPLLGPSKGRLPFPIAGILVVAATALLFALVRERGTGDASAEPRV
                     RLLESLKGIAGSGDKSALLILASLFLWFLGYQGVLPFIGKYSVESLGVSQGRAAFAAG
                     MVGIAYALFAIPSGYVAHRIGRKRAIRTALAALTVLVSLLFFLDPLASALGLAGMGKV
                     YLFWGLLFFFGLFWVTVVTNSFPMLWQMSRYGTVGIYTGLYYTFSQGAAILSPPLTGG
                     IIDLAGYRGIFAFCAVCMAAAFTVMGFVSGGEPEGAVAASGAAS"
     gene            complement(2160..3437)
                     /locus_tag="P5313_11670"
     CDS             complement(2160..3437)
                     /locus_tag="P5313_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HRY81066.1"
                     /translation="MGSNAPAQAKSFQKRLSELYGKTESASQAKRYLDLAQRASEAFG
                     GEPRFFSAPGRTELGGNHTDHNRGRVLCAAVTLDIAAAVVPSEGTEVELWSDGWERPF
                     RVDLARTDPVASERGTTEALIRGVAEGFAEAGYRTGGFKGILNSRVPAGSGLSSSAAL
                     EVLLGTIQSGLYNDGKVRPEELAGIGQRAENRHFGKPCGLMDQTASAVGGILVIDFEN
                     PGTPRIRKIKADFEKEGYALAVVSTGGSHADLTEDYAAIPREMREAASVFGKETLRGL
                     EPGQLLRKLPEVRAAAGDRAALRALHFLEENERVLSMAKALEDGRVKRFLKLAGRSGS
                     SSWRYLQNVLAPGDPRNQGLALALALSEFLLGKRGACRVHGGGFEGTLLAFIPLDGFK
                     EYRETMDRVLGPGCVTRLRIREEGAGEIRQKET"
     gene            complement(3624..3818)
                     /locus_tag="P5313_11675"
     CDS             complement(3624..3818)
                     /locus_tag="P5313_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81067.1"
                     /translation="MKQFAIRGTSQAPSVLELINEIENGYFVRIIHTHEDWEDVREEF
                     MTKELFDTCVRTGYIAELSA"
     gene            complement(4016..4813)
                     /locus_tag="P5313_11680"
     CDS             complement(4016..4813)
                     /locus_tag="P5313_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRY81068.1"
                     /translation="MKKILAIALILAVAGAAFAQAPKDGFYFAEQTEFSKSGWRYQAV
                     LEVKGGKIVSANWNGVNNLGLADKKTVAKAGQYGMAKVAKQGEWDVQAARVEAELVKV
                     GDVSKIAVKADGKTDAISGVSITVKEFLDLVPKALAAGPVAKGSYKKDGWFYAKDAAF
                     DRSGYAATALITIVNGRIVGAVSNAVHKDGGDSKYVRAVKGTYKMNAKQGEWNVQSEK
                     MAAALVKAQEVSKIAVKADGKTDAISGVSVTVGEFFKIAAEALKAAQ"
     gene            5275..6573
                     /locus_tag="P5313_11685"
     CDS             5275..6573
                     /locus_tag="P5313_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY81069.1"
                     /translation="MNPLAEELNRTLTGTCAARLLSASGTRMYFPKGILSQSAEASEK
                     AKVFNATIGMSYVKGQPMILDAIRAHLPGLSPVEAVAYAPTAGVPALRKRWKEELLRK
                     NPSLKGVPVSLPVVVPGLTAGVSFLADLFVDPGDAVVLPDLFWPNYRLILEERKGARL
                     ATYPMFTRSGGFNVEGFKAALETEGRVRGKVVTILNFPNNPTGYSPTLAEAEALVAAL
                     EQVAAGGTDILVVSDDAYFGLQYEEGTLRESLFARLAGRNPRILAAKVDGQTKEDYVW
                     GFRSGFLTFGCPGFQEDQYEALVKKVMGVVRSCVSNSAAPTQYILHKALETPGYEEQK
                     ARYRKILEERYRKVKDFLARHPAPACLRPLPFNSGYFMSFECDGVSAETLRKKLLDEK
                     GIGVIALQEKWLRVAFSSVEAEDVEPLYTEIFAAARALMG"
     gene            6601..7251
                     /locus_tag="P5313_11690"
     CDS             6601..7251
                     /locus_tag="P5313_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRY81070.1"
                     /translation="MYRNTAALLILVLCLPAAPLSAAEAETGEASWYGPGFHGRRTAN
                     GEVFDKEAMTAAHRTLPFGTLVRVRNLDNGREAVLRINDRGPFARGRILDVSEAGARA
                     LGMVESGTARVLIEVLRSGTAAPGAPAAGAAPDPAGSGPAVTVRIQVASYRDAANAGR
                     TAERLRLSGMNPSLETEGAYTRVVLTGISPSERDALVRRLADLGYPGVLVRTETRF"
     gene            complement(7325..8500)
                     /locus_tag="P5313_11695"
     CDS             complement(7325..8500)
                     /locus_tag="P5313_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRY81071.1"
                     /translation="MEDQYRFPLENYMSQEQFERIERFASDKETPCLIVDLDIVKKKY
                     AQLRALLPYAEVYYAVKANPMDEVVSTLRDLGSSFDVATRYELDQLLRLGVDPARMSF
                     GNTIKKEKDIAYFYEKGIRLYATDSTADVEKIARAAPGSRVFFRILTEGMGADWPLSR
                     KFGSHPDETRRLVLRAKDLGLEPYGISFHVGSQQRDIGQWSTALTTTGGIFKWAREEH
                     GIELQMVNMGGGFPSNYLEPTNAIEAYAEDIKNYLLNNFDGRWPRVIIEPGRSMAGDC
                     GVIVTEIVNIARKSLHDLYPWVFLDVGKFGGLIETLDEAIKYPIYFKGSGKQVDVILA
                     GPTCDSMDILYEKTTYMMPTSARIGDRAYILTTGAYTQSYSSIYFNGFPPLKAYVLD"
     gene            8605..9372
                     /locus_tag="P5313_11700"
     CDS             8605..9372
                     /locus_tag="P5313_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81072.1"
                     /translation="MTGFFIKKAFFDGWDHLWGLVALNLGALALLAGFVLAPLALELP
                     GGAVWPGLGFLVLSAYSLVCAEVLKDAAEYRNLSLPAFREALKRRGVLGLAFGAALSA
                     ALVLFWISVPFYLSRGSFLGWFAAGTLFWCFLIALLALQWFPAVRALLPGTVKASVRK
                     CFLLLADNLGFSILLALYSAAGLVVSVFSAFLVPGLSGTVLGSVVALRLVLKKYDWLE
                     ANPGAKRGDVPWESLLSEDKELVGKRSLKGMLFPWKE"
     gene            9449..10432
                     /locus_tag="P5313_11705"
     CDS             9449..10432
                     /locus_tag="P5313_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY81073.1"
                     /translation="MSITTSQQIAKWYDVYKSIDVTLTKEILKTTGLDPRHVFMKCVG
                     EQWPCVIYSTSFSGAKIIVSAKPILLEKIKRANNLVSLRFSFRFEGKADPLSFFVTGR
                     ATGFAPYQAGGELQFMTLEYTQRPPDDLIEIMGRLLEANINSARRREERILLNPDSMR
                     RLCLMSKDTILTVQGVPRKCIVRDLSFSGAKLIIVGLAKFLVGKECLLRLEMDEPRET
                     LDLKGSIVRYEDVEGRKDLTAVAVRFEDQTVPMTYKIHINDFLGQVRQVRPENAPGAE
                     GASTAQPAAARPAPAQPAPAQAASAQPAESPAPAGPAPAKAGGGVPHGQSR"
BASE COUNT         1730 a         3496 c         3459 g         1815 t
ORIGIN      
        1 gtgagccaga cgttgccccc gatggcggag ccccggccga taacggtctg gccccccagg
       61 atggtggccc ccgagtagat cgtcacgtcg tcctcgatgg tgggatggcg cttcacgtcc
      121 ccctcggcct tgcgcaccga gagggcaccc agggttaccc cctggtagat cttcacccgg
      181 tccccgatga ccgcggtctc cccgatgacc acccccgtgc cgtggtcgat gaagaacatc
      241 cgcccgatcc gggccccggg gtggatgtcg atcccggtct tgccgtggag gtattcgctc
      301 atcatccggg gcaggagggg gatccggcgt tcccagaacc agtgggcgaa gcggtgggcc
      361 acgatggcct ccatgccggg ataggagagg atgacctcct ccacgctctt ggccgcggga
      421 tccccggaga aggcggcggc cacgtcctcc atcagcacgg tccggatgcc cggcagggat
      481 tccaggaagt cgtaggccag gtcccgggcc tccatgtggc aggccgcggg acagccccgg
      541 ccggtctcca tccggagccg gtagtccagg ctgcgctcca gctcccggac gaggttccgg
      601 gccgtcctgt ggacgagctc tccgatcagg aagtcggcgt tcgtgtccac gatgccgtcc
      661 tccctccggt agcccgggaa ggcaagggtc tccaggtcgt ccacgatggc ggccacgctc
      721 tgcttggagg ggaggttggg gccgtcggag tggttgatgc ccccggcctc ctcgtaggag
      781 gccaggagct tgcggatcgt ggaggccagg gcgtccatca ggagggcccc ggcgtcggac
      841 gccttttcat gaagcggccc ccgatgccgc gacggcccct tcgggctccc cgccgctcac
      901 gaagcccatg accgtgaagg ccgcggccat gcatacggcg cagaaggcga agatcccccg
      961 gtagccggcc aggtcgatga tcccgccggt caggggaggg gagaggatgg cggccccctg
     1021 gctgaaggtg tagtacaggc ccgtgtagat cccgacggtg ccgtaccggg acatctgcca
     1081 gagcatgggg aaggagttcg tcaccacggt cacccagaac aatccgaaga agaacaggag
     1141 accccagaag aggtagacct tgcccatccc ggccagtccc agggcggacg cgaggggatc
     1201 caggaagaag agtagggata ccaggaccgt gagagccgcc agggccgtcc ggatggcccg
     1261 cttccgtccg atccggtggg cgacatagcc cgaggggatc gcgaacaggg cgtaggctat
     1321 acccaccatg cccgccgcga aggcggcccg cccctgggaa acccccaggg attcgacgct
     1381 gtactttccg atgaagggaa ggaccccctg gtaccccagg aaccacagga agagggacgc
     1441 caggatcagg agggcgctct tgtccccgct gcccgcgatg cccttaaggg attccagcag
     1501 gcgtacccgg ggctccgcgg acgcgtcccc ggtcccgcgt tcccgcacca gggcgaagag
     1561 cagggccgtc gccgccacga ccaggattcc cgctatcggg aagggcaggc ggcccttgga
     1621 gggccccagg agggggagcg tgacgtccag gtccatgagc cgggccagcc ccagggtacc
     1681 cacgatgctg gcgatgccgc ccatggtgtt gatgacgccg ttggcctcgg agcggaattc
     1741 ccccggcacg atgtccggca tcaaggccac cacggggccc cgggccgact gcttccagat
     1801 gttgagcagg aagatcgccg ccaccagggc cgcgagggag gccgcggccg cgtaggggag
     1861 ccaggcgaag cagaccgccg tcaggggcag ggtgaccagg atgaagggca tgcgccgccc
     1921 gatcggggaa cggatcttgt cggaccagac cgcgacgatg gggatcagga agagggcaaa
     1981 aatgttgtcc agggtcatga cggaccccac gatgcccatg gatcggatgt atttgcccag
     2041 gaagatgggc acgtaggtgt cgtagagggg atccatgagc cccatcgtga agaaaccgaa
     2101 gccgatcagg aaagtcgtgc ccaggaaggg ccggaagctg ttcgggacct ttgtcatcgc
     2161 tacgtctcct tttgccggat ctcccccgcg ccttcttccc gtatcctcag ccgggtcacg
     2221 cagcccggac ccaggacgcg gtccatggtt tcccggtact ccttgaaacc gtccaggggg
     2281 ataaaagcca ggagggtgcc ctcgaagccg cctccgtgga cgcggcaggc gccccgcttg
     2341 cccagcagga attcggaaag ggccagggcc agggccagcc cctggttccg ggggtccccg
     2401 ggcgccagga cgttctgaag atagcgccag gaggaggatc cggagcggcc ggccagcttc
     2461 aggaaccgct tcacccggcc gtcctccagg gccttggcca tggacaggac ccgctcgttc
     2521 tcctccagga aatgcagggc ccggagggca gcccggtctc ccgccgcggc gcggacctcc
     2581 ggcagcttcc gtaggagctg gccgggttcc aggccccgga gggtctcctt gccgaagacc
     2641 gaggcggcct cgcgcatctc cctcggaatg gccgcgtagt cctcggtcag gtccgcgtgg
     2701 ctgccccccg tggataccac ggccagggca tagccttcct tttcgaaatc cgccttgatc
     2761 ttccggatcc ggggagtccc gggattctcg aaatcgatga ccaggatccc gcccaccgcg
     2821 gacgcggtct ggtccatgag cccgcagggc ttgccgaagt gccggttctc cgcccgctgg
     2881 cctatcccgg cgagctcttc gggccgtact ttgccgtcgt tgtataatcc cgactggatt
     2941 gtcccgagca ggacttccag ggccgcggaa ctggacagtc cggagcccgc cggcacccgg
     3001 gagttcagga tgcccttgaa gccccccgtg cggtaccccg cctcggcgaa gccctcggca
     3061 accccccgta tcagcgcctc cgtggtgcct ctctcggagg ccaccggatc cgtccgggcg
     3121 aggtccacgc ggaaggggcg ctcccagccg tcggaccaga gctccacctc ggtgccctcg
     3181 gagggcacca cggccgcggc gatgtccagg gtgaccgcgg cgcagaggac ccggccgcgg
     3241 ttgtggtccg tgtggttccc gcccagctcg gtgcggcccg gcgcggaaaa gaagcgaggc
     3301 tcgccgccga aggcctcgga ggcccgttgg gcaaggtcaa ggtaccgctt cgcctgggag
     3361 gccgattcgg tcttgccgta gagttcggaa agacgtttct ggaagctttt cgcttgggcg
     3421 ggggcgttcg agcccatgga gtcctcccga cgcgtcccgc cgcgcagcgc agcggacgcc
     3481 gatcggggac aggatatcat ccgggagact ggaagggaag cagtcaggag tcaggagtca
     3541 gggaaccgtc ggacggcggc ggattccttc tcgacgaccg gcgacgggag ttcgacccgt
     3601 cgcccggcgt ctgactcctt gtttcaggcg gacaattcgg cgatgtatcc ggtgcggacg
     3661 caggtgtcga agagttcctt ggtcatgaac tcttcccgga cgtcctccca gtcttcgtgg
     3721 gtatggatga tgcggacgaa gtatccgttt tcgatctcgt tgatgagttc caggacggag
     3781 ggcgcctggc tggtgccccg gatggcgaac tgtttcatga gcttcctcct gggaaggccc
     3841 gccggccatt ccgatgccgg gcagacttac cgtttcaatt ttcggaggat gcaggctttc
     3901 ggcttagcgc caaatccggg tgccgcggcg ggaaaaagga acgcccggga cgaagggtcc
     3961 cgggcgtatc caacagagtc cggaacggcc ggattcgagc cggccggatt cgggcttact
     4021 gggcggcctt gagggcttcc gcggcgatct tgaagaactc gccgacggtc acggacacgc
     4081 cggagatggc gtcggtcttt ccgtccgcct tcacggcgat cttggagact tcctgggcct
     4141 tgaccagggc ggcggccatc ttctcggact ggacgttcca ctctccctgc ttggcgttca
     4201 tcttgtaggt gcccttgacg gcgcggacgt acttgctgtc gcccccgtcc ttgtggacgg
     4261 cgttggaaac cgcgccgacg atgcggccgt tcacgatggt gatcagggcc gtggcggcgt
     4321 acccgctgcg gtcgaaggcg gcgtccttgg cgtagaacca gccgtccttc ttgtaggaac
     4381 ccttggcgac ggggcccgcg gccagggcct tggggaccag gtccaagaat tccttcacgg
     4441 tgatggacac gccggagatg gcgtcggtct tgccgtccgc cttcacggcg atcttggaaa
     4501 cgtccccgac cttgacgagt tcggcttcca cccgggcggc ctggacgtcc cactcgccct
     4561 gcttggcgac cttggccatg ccgtactggc cggccttggc gacggtcttc ttgtccgcca
     4621 gcccgaggtt gttgacgccg ttccagttgg cggacacgat cttgccgccc ttcacctcga
     4681 ggacggcctg atagcgccag ccgctcttgg agaactcggt ctgctcggcg aagtaaaagc
     4741 cgtccttggg cgcctgggcg aacgcggcgc ccgcgacggc caggatcaac gcgatcgcga
     4801 gaatcttctt catatgcact ccctcctgcg agatagcaat aaaactatat agatataatg
     4861 gacagtgaat atccattatg tcaagggctt tgaggcggcg aagccgccgc aaaaggcaac
     4921 gaagccctgc gggtttcgtc gatccacaca aaaagatcct atggccggtg aacggtctac
     4981 agtgcccgat taccgattcc tgtcatcgga gcgtggaagt aggccgcgaa ctgtcctatc
     5041 ttccgtccac tggccaccgt ccactgtcca cttcccgttg acactcacag taacctccta
     5101 tacggtttag caggttgttg aaatactcga ggcatttcaa caacctgcta tgcaattgct
     5161 cgagaacctt acggttctct gcgcaattgc gacattaagg aagtgttgaa aaggctctat
     5221 agcctttttc aacaccctgt tagagcaacg aagcacccaa atcaggaggc ctccatgaac
     5281 cctctagcgg aagaactgaa ccggaccctt acgggaacct gcgccgcccg cctgctatcg
     5341 gcctcgggca cccggatgta tttccccaag ggcatccttt cccagtccgc ggaagcctcg
     5401 gaaaaggcca aggtttttaa cgccactatc ggcatgtcct acgtgaaggg ccagcccatg
     5461 atcctggacg cgatccgggc ccaccttccc gggctttccc ccgtcgaggc ggtggcctac
     5521 gcccccaccg cgggcgtgcc cgcgctccgg aagcggtgga aggaggagct cctccggaag
     5581 aacccctccc tgaagggggt tccggtctcc ctgcccgtgg tggtgccggg cctgaccgcc
     5641 ggcgtctcct tcctggcgga cctcttcgtg gatcccggcg acgcggtggt gctgccagac
     5701 ctcttctggc ccaactaccg gctcatcctg gaggagcgca agggcgcccg cctggccacc
     5761 tatccgatgt tcacccgtag cggcggcttc aacgtggagg gcttcaaggc ggccctggag
     5821 accgagggcc gggtccgggg caaggtggtg accatcctga acttcccgaa caaccccacc
     5881 gggtactccc cgaccctggc ggaggcggag gccctcgtgg ccgccctgga gcaggtcgcc
     5941 gcgggaggga cggacatcct ggtggtctcg gacgacgcct atttcggcct ccagtacgag
     6001 gaaggcaccc tgcgggagtc cctcttcgcc cggctggcgg ggcggaaccc ccgcatcctg
     6061 gccgccaaag tggacgggca gaccaaggag gactacgtct ggggcttccg gtcgggcttc
     6121 ctgaccttcg gctgtccggg gttccaggag gaccagtacg aagccctggt caagaaggtg
     6181 atgggggtgg tccgttcctg cgtgtccaac tccgcggccc cgacccagta catcctgcac
     6241 aaggccctgg agacccccgg ctacgaggag cagaaggccc ggtaccggaa gatccttgaa
     6301 gagcggtacc ggaaggtcaa ggatttcctg gcccggcatc ccgccccggc ctgcctccgg
     6361 ccccttccct tcaactccgg gtacttcatg agcttcgagt gcgacggggt atccgcggag
     6421 accctgcgga agaagctcct ggacgagaag gggatcggcg tcatcgccct gcaggagaag
     6481 tggctgcggg tggccttctc cagcgtggag gcggaggacg tcgagcccct ctacacggag
     6541 atcttcgccg ccgctcgggc cctgatggga tgagcccggc ccgccgatac tgaatccagg
     6601 atgtatcgga ataccgccgc tctgctgatc ctggtcctct gcctcccggc cgcccccctc
     6661 tccgcggcgg aggcggagac cggggaggcc tcctggtacg gcccgggctt ccacggacgc
     6721 cggaccgcca acggggaggt cttcgacaag gaggccatga ccgccgccca tcggaccctg
     6781 cccttcggca ccctggtgcg ggtgcggaac ctggacaacg gccgggaggc ggtcctccgg
     6841 atcaacgacc gcgggccctt cgcccggggc cggatcctgg acgtgtccga ggcgggggcc
     6901 cgggcactgg gcatggtgga gtcgggaacc gcccgggtcc tgatcgaggt cctgcgctcc
     6961 ggaacggccg cgccgggagc ccccgcggcg ggggccgccc cggacccggc gggctccggc
     7021 ccggccgtga cggtccgcat ccaggtggcc tcctaccggg acgctgcgaa cgccgggcga
     7081 acggcggagc gtctccggct ttccggtatg aacccctccc tggagaccga gggtgcctac
     7141 acccgggtgg tactgacggg catctcgccc tcggaacggg acgccctggt taggcgcctg
     7201 gccgacctcg ggtatccggg ggtcctggtg cggacggaga cccggttctg aaaggtccgc
     7261 gggcatcgta aaataaaaag ccgccggcga tgccggcggc tttttttgtg atcggcgaac
     7321 cgggtcagtc caggacgtag gccttcaggg gcgggaagcc gttgaagtaa atggagctgt
     7381 agctctgggt gtaggccccg gtggtcagga tgtaggcccg gtccccgatg cgggcgctgg
     7441 tgggcatcat gtaggtggtc ttctcgtaca ggatgtccat ggagtcgcag gtgggcccgg
     7501 ccaggatcac gtccacctgc tttccggagc ccttgaaata gatcgggtac ttgatggcct
     7561 cgtccagggt ttcgatgagg cccccgaact tgccgacgtc caggaagacc caggggtaca
     7621 ggtcgtgcag ggacttccgg gctatgttca cgatctccgt cacgatcacg ccgcagtccc
     7681 ccgccatgga ccgtccgggc tcgatgatga cccggggcca gcgtccgtcg aagttgttga
     7741 gcaggtagtt cttgatgtcc tcggcgtagg cctcgatggc gttcgtgggc tccaggtagt
     7801 tggaggggaa gcctccgccc atgttgacca tctggagctc gataccgtgc tcctcccggg
     7861 cccacttgaa gatccccccc gtggtcgtga gggcggtgga ccactggccg atgtcccgct
     7921 gctgactgcc cacgtggaag gagatcccgt agggctccag gcccaggtcc ttggcgcgca
     7981 ggaccaggcg gcgggtctcg tcggggtggc tcccgaactt tcgggacagg ggccagtccg
     8041 ctcccatgcc ctcggtcagg atccggaaaa agacccggga acccggggcg gcccgggcga
     8101 tcttctccac gtccgcggtg gaatccgtgg cgtagagccg tatccccttc tcgtagaagt
     8161 aggcgatgtc cttctctttc ttgatggtgt tcccgaagct catccgtgcg ggatccacgc
     8221 ccagccggag gagctggtcc agctcgtatc gggtggccac gtcgaagctg gaacccaggt
     8281 cccggagggt ggagaccacc tcgtccatgg gattggcctt gacggcgtag tagacctcgg
     8341 cgtagggcag gagggcccta agctgggcgt actttttctt gacgatgtcg agatcgacga
     8401 tcaggcaagg ggtttccttg tcgctggcga accgctcgat ccgctcgaac tgttcctggg
     8461 acatgtagtt ttcgagcgga aaacggtact gatcctccat gggcctgcct ctggggatgt
     8521 cggatgaccg tcctataaaa acggcatagc cggagcatag ggacgggctt gccactttgt
     8581 caaggatgcg gtaaaccgtc gattatgaca ggctttttca tcaaaaaggc gttcttcgac
     8641 ggctgggacc acctctgggg cctggtggcg ctgaacctcg gcgctctggc gctcctggcc
     8701 ggtttcgtcc tggcgcccct ggcgctggag cttcccggag gtgcggtctg gccgggcctg
     8761 ggcttcctgg tcctgtccgc ctactccctc gtatgcgcgg aggttctcaa ggacgccgcg
     8821 gaataccgga acctctccct ccccgcgttc cgggaagccc tgaagcgccg cggggtactc
     8881 ggtctggcct tcggggcggc gctctccgcc gccctcgtcc tgttttggat ctccgtgccc
     8941 ttctacctct cccggggaag cttcctgggc tggttcgccg cgggaaccct cttctggtgc
     9001 ttcctgatcg ccctcctggc cctccagtgg ttccccgcgg tccgcgccct cctcccgggg
     9061 accgtgaagg cctcggtccg caagtgcttc ctgctcctgg cggacaacct ggggttctcg
     9121 atcctcctgg ccctctattc cgccgcggga ctcgtggtgt ccgtcttctc cgccttcctg
     9181 gtcccggggc tctcggggac cgtcctggga tccgtggtcg ccctgaggct tgtcctcaag
     9241 aagtacgact ggctggaggc caacccggga gcgaagcgcg gggacgtccc ctgggagtcc
     9301 ctcctctcgg aggacaagga gctcgtcggc aagcgcagcc tcaagggcat gctctttccc
     9361 tggaaagaat aagccgggaa ggcgcggccc gggcggccct cgtcgttgac caaggcccct
     9421 ccgatgccct attattccgc caaggcctat gtccatcacc accagccagc agatcgccaa
     9481 atggtatgac gtctacaaga gcatcgacgt cacgctgacg aaggaaatcc tcaagacgac
     9541 gggcctcgac cccaggcacg tcttcatgaa gtgcgtcggg gagcagtggc cctgcgtcat
     9601 ctactcgacc tccttctccg gggccaagat catcgtcagc gccaagccca tcctcctcga
     9661 gaagatcaag cgcgccaaca acctcgtctc cctgcgcttc agcttccgct tcgagggcaa
     9721 ggccgatccc ctgagcttct tcgtcacggg ccgggcgacg ggcttcgccc cctaccaggc
     9781 gggcggcgag ctccagttca tgaccctcga gtacacgcag cgccccccgg acgacctcat
     9841 cgagatcatg ggccgcctcc tcgaggccaa catcaactcg gcccggcgcc gcgaggagcg
     9901 catcctcctc aatccggact cgatgcgcag gctctgcctc atgtccaagg acacgatcct
     9961 caccgtccag ggcgtgccgc ggaagtgcat cgtccgagac ctgtccttct ccggcgcgaa
    10021 gctcatcatc gtcggcctcg ccaagttcct ggtcggcaag gagtgcctcc tccgcctgga
    10081 gatggacgag ccgcgggaga ccctggatct caagggctcg atagtccgct acgaggacgt
    10141 cgagggccgg aaggacctca ccgccgtcgc cgtgcgcttc gaggaccaga ccgtcccgat
    10201 gacctacaag atccacatca acgacttcct gggccaggtc aggcaggtca ggcccgagaa
    10261 cgcccccggc gccgagggcg cctctacggc ccagcccgcg gccgcgaggc cggcccccgc
    10321 tcagcctgcc cccgcccagg cggcctcagc acaaccagcc gaatcccccg ccccggcggg
    10381 accagctcca gccaaggcgg gtggaggagt accccatggc caatcccgct agggacgagg
    10441 aggccggcga ggccgccctc cgccgtctcg tcttcatcga ccttcccccc gccctggcgc
//
LOCUS       DAOTEI010000124        10471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4473_length_10471_cov_5.858199, whole genome shotgun sequence.
ACCESSION   DAOTEI010000124 DAOTEI010000000
VERSION     DAOTEI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4473_length_10471_cov_5.858199"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="P5313_11710"
     CDS             <1..215
                     /locus_tag="P5313_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY81074.1"
                     /translation="LVIGALTDAVHEVVDIDDEHMEPAPRFGTRIDSGFIRAIGKRGD
                     AFVIILDIDKAFRGDETEELARAAAS"
     gene            complement(287..1423)
                     /locus_tag="P5313_11715"
     CDS             complement(287..1423)
                     /locus_tag="P5313_11715"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY81075.1"
                     /translation="MDRKKILMTMIEVGFGHKAPALAVRDAIVERVGDAVQVDVVDFA
                     RECGALKDDRALKDSWDLGLAFPLSARIGYLLVELYGKGAEYIDLLFKDFVHKGIEYI
                     DRYRPDLVFATHPLALYVAVKAKETLRRPFKVIAYVVDPFDGYAWWANEGADALLVAS
                     DYSRDRLLRHGVRPDIIRKMGFPINKKFFEIRTPPPEILRDLDLNPEWPTLLVTAGGQ
                     GIGKVFAYAQALYLFQAPVNLIVVTGRNKATLRRLESMKAKVLSRTRLAPLGYVSNMN
                     ELMAASDVVVGKAGASTAMEAAFMSKPFIFTEWATYNDRFIINFALNYHVGWYCPTVF
                     AFSQVVRKIVDTDVLAKYRSNLENLSVEPGTDEVADYLLKEAGA"
     gene            complement(1531..2877)
                     /locus_tag="P5313_11720"
     CDS             complement(1531..2877)
                     /locus_tag="P5313_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81076.1"
                     /translation="MAAKRVNARAGAEPRAWVAAANMGLGHKRAVRPLSDLAEGGILI
                     VNDPGFADPEELKLWERLLSIYEKLSRARGIPLIGPALFGILDYFQRIRPPYPRVDLS
                     VPTIQTRFVDRMLEKGLCRTLLAKIKEKPLPLVTSFYHPAVAADRAGWGRIYCIICDA
                     DINRVWVPSRPRDSRIEYLVPCGSAMRRLRQYGVPDERIWMTGFPLPLSLLGDESLSV
                     LRRDLGRRLRRLDPEDRFRPLHGRNVEHFLGPENFLEGRDPGPVTITFAVGGAGAQKE
                     IGTAALESLAPAIRTGEFAMNLVCGVRSEVREYFEEAVRRILPDHPGVRVVSGRTDSE
                     YFEAFESCLHDTDVLWTKPSELSFYVGLGIPLIAAPTIGSQEDFNLRWLREIQATLHQ
                     SDPRYALEWIRELLEQGRFAEAAWSGFLKARKYGTYKIRELLATGTMERESNPLRR"
     gene            3090..4841
                     /locus_tag="P5313_11725"
     CDS             3090..4841
                     /locus_tag="P5313_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY81077.1"
                     /translation="MKDQLIVKNLPFIKILPAWAQELSYKYLSKTANLYILHGNIRDF
                     LPHKMNEGEFIFVKIQDYVSEVLFGNRDVIAFWDRSSGVTFCTPEMMKAYLEAHVERN
                     PDVDKDDLVSPDPLLAFKYLEEYFLLNIPKKKRIVLIADYAETFLPASDVSRLSDEDR
                     YCFVTLNRWSHDPVFTKGDVSVILLTENLTDINPRIVSSPTTVKVQIPIPDESVRISF
                     LEFLERRKTLLLDKGLGPREVGTITSGLNLMNLNRLAAESYQEDLPISMDYLKQKKKE
                     IIENEASGLLEFLETEHDLSYVSGHDFVKRRFKSAARALKQGRLDVLPMGYLISGPVG
                     TGKSFLVTAFAGEIGIPMVKFKNFRSKWQGVTESNLEKVLNILKAMAPVAVMIDEADA
                     FLGDRNQEGDSGTSNRIFAQIASFMGNTEYRGRIIWFLITCRPDLLPIDLKRQGRAEE
                     HLALFYPDTDAEKEALFDTLVRKLDLSIRKFPVGDLLRRFKYEFSGADLEAVLIRAKF
                     RAAMDGRSFVTREDMDEILADFVPPAYPHEIELQNLVAVLECTSKEMVPKRFQNLDRT
                     KLVRDIREIKELLGERE"
     gene            complement(5419..6099)
                     /locus_tag="P5313_11730"
     CDS             complement(5419..6099)
                     /locus_tag="P5313_11730"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HRY81078.1"
                     /translation="MDDKRTARYLGILLVSAIACGILNTVPALEYPDYLEKLPSIRTW
                     VLTAVFFQAVMATIYVYIAVLFNQVLRKRRDVLATVYLVFRIVGAAFLYVGIGSLLLL
                     LMLSRRFAAEAPSDPSYYRTLGELLRQGRDHMNHIGMVLPWMTGGLILCYGLFKDRLV
                     PRWLSVWGFTGAALTILATVMLMLEFIKMATPIYFALNVPTALFELTLAARLAFRGFD
                     EDVRPAVR"
     gene            complement(6138..6368)
                     /locus_tag="P5313_11735"
     CDS             complement(6138..6368)
                     /locus_tag="P5313_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY81079.1"
                     /translation="MAARTLVTNNIRSLRFAAGEMTQAELADRVSLTRQTIIAIEQGR
                     YSPTLETAFQIARVFGVPLEEVFQYPDERADS"
     gene            complement(6371..6823)
                     /locus_tag="P5313_11740"
     CDS             complement(6371..6823)
                     /locus_tag="P5313_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81080.1"
                     /translation="MPFEEKITWVNAVVSAAVAAVYVALVLKRMESVPVDQVSYQGPL
                     LIAIGSVIVMSILGAILVAVAGSVSARIKGDGSEAEVNRSDQRDTEINRRGELVGYYV
                     SSGGALGALALAMLRYDPFWIANALYLSFVAGALVSSASKLIAYRRGF"
     gene            7115..8491
                     /locus_tag="P5313_11745"
     CDS             7115..8491
                     /locus_tag="P5313_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HRY81081.1"
                     /translation="MDSIVVGLKSALAAFFGFKAYVILPAFMLVLALAARMRPKEALF
                     ASIKIGAGFSGVFIVFSFFVTQIRPAIEAVIALRGLNYPVADVGWPPLAAITWSSPLT
                     PLLIPLVLVLNLVMIAARSTKTLYIDLWNYWHFALIGVLVRNATGSLVLGLAAGLLLA
                     LYTIKTAEWSAPYVRRETGLEGLAVSPVSVAGLLPYAVALNRAFDAIPGLRRLSWDPS
                     RTGAEGSGPRLLQEPMVIGILVGTGLSLLAGYDLKGVLETSVNIAAVMFLLPKCGALI
                     GEGMGAVSLAFKLLVERRFRSLRGLSIAVDTGILMTNPSVMATGLILIPVSLLVAFLL
                     PGNRVLPLGDLPNLISIMSLCTLVMGGNVLRSVLAGVPVIASFLLIASDMAPLYTEQA
                     AAAGMNLDLGGRELTAFTDGGNQLRYWLYGLFQGRAAAWAAVPLVLGLLWLAGREHKK
                     MADLEPPR"
     gene            8595..9476
                     /locus_tag="P5313_11750"
     CDS             8595..9476
                     /locus_tag="P5313_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRY81082.1"
                     /translation="MRHSSFPRSLACILALTLGLSGNLAAQEEFIRLPGGGFLMGSPV
                     TEKQRNPDEGPQHPVKVAPFTMGRYEVTQAEWTALMENNPSRFRGDRLPVEGVSWYEA
                     LVYCNKRSIAEGLDPCYTIQGSADPADWGSVPTSSNGAWNAVVCDFSADGYRLPTEAE
                     WEYACRARTESATAFGDSLGPDRANFDGSRPYNSEILAEPLRRTTPVGSYPANPWGFH
                     DMHGNVWEWCWDHYADKYYARSPDTDPRGAEGGRYRVLRGGAWGNFGYRLRSAARRDE
                     DPFYRDADNGFRLVRGR"
     gene            complement(9623..9994)
                     /locus_tag="P5313_11755"
     CDS             complement(9623..9994)
                     /locus_tag="P5313_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY81083.1"
                     /translation="MDLGAFSVSLAVKNVEASRAFYEKLGFSAFGGDAAQGWLILKNG
                     VCILGLFQGMIPRNTLTFNPGWDQDAQALPSFTDIREIQKELKRKGVRLVTEADESTS
                     GPASFTVEDPDGNPVLFDQFV"
     gene            10182..>10471
                     /locus_tag="P5313_11760"
     CDS             10182..>10471
                     /locus_tag="P5313_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011910143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81084.1"
                     /translation="MERTSLTHPLIVDSVETPGGGRIGLTFCPGKKQARAASGSWNRD
                     LDADLDAIRAWGAEVLVSLIEPAEYQRLGVEDLPEKARARMAHLALPVPDYGL"
BASE COUNT         1904 a         3489 c         3251 g         1827 t
ORIGIN      
        1 ccctggtgat cggggccctg acggacgcgg tccacgaggt ggtggacatc gacgacgagc
       61 acatggagcc ggctccccgc ttcggtaccc ggatcgattc gggcttcatc cgggccatcg
      121 gcaagcgggg ggacgccttc gtcatcatcc tggacatcga caaggccttc cggggggacg
      181 agaccgagga gctcgcccgg gccgccgcct cctgaggccg gcgcagacca ttggaggaaa
      241 ctcgccgaag cacagcttcg gcgccctgag gccggcgcag ccggcctcag gctccggcct
      301 ccttcaacag gtagtcagcc acctcgtccg tgccgggttc cacggagaga ttctccaaat
      361 tactcctgta tttggccagg acatccgtat cgacgatctt gcgaacgacc tgagaaaagg
      421 cgaagaccgt ggggcagtac catccgacat ggtagttcag ggcgaaattg atgatgaacc
      481 ggtcgttgta ggtggcccat tcggtgaaga tgaagggctt gctcatgaaa gccgcctcca
      541 tggccgtgga cgccccggcc ttgcccacca ccacgtccga ggccgccatc agctcgttca
      601 tgttggagac atagcccagg ggagccagcc gggtccggga caggaccttg gccttcatgc
      661 tttccagccg acgcagggtc gccttgttcc ggccggtcac cacgatcagg ttaaccgggg
      721 cctggaagag gtagagggcc tgggcgtagg cgaagacctt cccgatcccc tgccctcccg
      781 cggtcaccag gagcgtcggc cactcggggt tcaggtccag gtcccgcagg atctccggag
      841 gcggggtacg gatctcgaag aacttcttgt tgatcgggaa gcccatcttc cggatgatgt
      901 ccggccggac tccgtgccgg aggagccggt ccctggaata gtccgaagcc accaggaggg
      961 cgtccgcccc ctcgttggcc caccatgcgt acccgtcgaa ggggtccacc acgtaggcga
     1021 tcaccttgaa gggccgcctc agggtctcct tggccttgac cgccacgtag agggccaggg
     1081 ggtgggttgc gaagaccagg tccggccggt agcggtcgat atattcgatc cccttgtgta
     1141 cgaagtcctt gaacagcagg tcgatgtact ccgccccctt gccgtagagt tccacaagga
     1201 ggtagccgat ccgggcggag agggggaagg ccaggcccag gtcccaggag tccttgaggg
     1261 cccggtcgtc cttgagggcc ccgcactccc gggcgaagtc caccacatcc acctggaccg
     1321 cgtcgcccac cctctccacg atggcgtccc ggaccgccag ggccggggcc ttgtggccga
     1381 acccgacctc gatcatcgtc atgaggatct tcttcctatc catgggttcg ggacctccgg
     1441 ggagcgcacg gaatgactgc ggggactcga tcagaatata cccataggca tggtgcgccg
     1501 gccactggac gccgtcatcc cccgcccgga tcagcgcctc aagggattcg actccctctc
     1561 catggtcccg gttgccaaaa gctcccggat cttgtaggtc ccgtacttgc gggcctttaa
     1621 aaacccgctc caggccgcct ccgcaaagcg cccttgctcc aacagctccc ggatccactc
     1681 cagggcgtag cggggatccg actggtgcag ggtggcctgg atctcccgga gccagcggag
     1741 gttgaagtcc tcctggctcc ctatggtcgg ggccgcgatc aggggtatcc ccaggcccac
     1801 gtagaaggag agctccgagg gcttggtcca caggacgtcc gtgtcgtgca ggcaggattc
     1861 gaaggcctcg aagtactccg agtccgtgcg gcccgagacc acccggactc ccggatgatc
     1921 cgggaggatc cgccggaccg cctcctcgaa gtattcccgc acctccgagc gcaccccgca
     1981 gaccaggttc atcgcgaact cgccggtccg gatggcggga gccaggctct ccagggccgc
     2041 cgtcccgatc tccttctggg cccccgcgcc ccccaccgcg aaggtgatcg tgaccgggcc
     2101 cgggtccctt ccttccagga agttctcggg acccaggaag tgttccacgt tccggccgtg
     2161 caggggacgg aaccggtcct ccgggtccag gcggcgcaga cggcggccca ggtcccggcg
     2221 caggacggag agactctcgt cccccaggag gctcagggga aggggaaagc ccgtcatcca
     2281 gatccgctcg tcgggcaccc cgtactgccg gagccgccgc atggcggacc cgcagggcac
     2341 caggtactcg atccgggaat cccggggacg ggagggcacc cagacccggt tgatgtccgc
     2401 gtcgcagatg atgcagtaga tccggcccca gcccgcccgg tccgcggcca ccgcggggtg
     2461 gtagaaggag gtgaccaggg gcagaggctt ctccttgatc ttggccagga gggtccggca
     2521 cagccccttc tccagcatcc ggtccacgaa gcgggtctgg atggtcggta ccgacaagtc
     2581 gacccggggg tagggaggtc gtatccgctg gaaatagtcc aggatcccga agagggccgg
     2641 gccgatcagg gggatgcccc gggcccggga aagtttttcg tagatcgaaa ggaggcgctc
     2701 ccagagcttc agctcctccg ggtccgcgaa gcccgggtcg ttgacgatga ggatcccgcc
     2761 ctccgccagg tcggaaaggg gccgcaccgc ccgcttgtgc cccagcccca tgttcgccgc
     2821 cgccacccag gcccggggct cagcccccgc ccgggcgttc acgcgcttag ccgccatgtc
     2881 gacctcccgt gacgggatga attttatgcg ggcggcgtcg ggaagtcaaa ggtggtgaat
     2941 aggtgaatcg tgaataggtg aataggaggg gtgccgggta ttccggtgaa ggcagttcgc
     3001 ggattatcga aatccgaggc gcggctccat tcacccattc acccattcac ccattcaccc
     3061 attcacccat tcactctata cttcccctca tgaaagacca gctcatcgtc aagaacctgc
     3121 ccttcatcaa gatcctcccg gcctgggcgc aggagctgtc ctacaagtac ctgtccaaga
     3181 ccgcgaacct ctacatcctc cacgggaaca tccgggactt cctgccccac aagatgaacg
     3241 agggggagtt catcttcgtg aagatccagg actatgtgtc cgaggtcctc ttcgggaacc
     3301 gggacgtcat cgccttctgg gaccggtcca gcggggtcac cttctgcacc cccgagatga
     3361 tgaaggccta cctggaggcc cacgtcgagc ggaaccccga cgtggacaag gacgacctcg
     3421 tctctccaga tcccctcctg gccttcaagt acctggagga gtacttcctt ctcaacatcc
     3481 ccaaaaagaa gcggatcgtc ctgatcgcgg actacgcgga gaccttcctg cccgccagcg
     3541 acgtgtcccg cctgtccgac gaggaccggt actgcttcgt caccctgaac cgctggtccc
     3601 acgaccccgt gttcaccaag ggggacgtgt cggtgatcct cctgaccgag aacctcacgg
     3661 acatcaatcc ccggatcgtc tcctccccca ccaccgtcaa ggtccagatc cccatacccg
     3721 acgagtccgt gcggatcagc ttcctggagt tcctggagcg ccggaagacc ctcctcctgg
     3781 acaagggcct ggggccccgg gaggtgggga ccatcacctc gggcctcaat ctcatgaacc
     3841 tgaaccgact ggccgcggag tcctaccagg aggacctccc catctccatg gactacctca
     3901 agcagaagaa gaaggagatc atcgagaacg aggcctcggg cctcctggaa ttcctggaga
     3961 ccgagcacga cctgtcctac gtctccggcc acgacttcgt gaagcgacgc ttcaagagcg
     4021 ccgcccgggc actcaagcag gggaggctgg acgtcctgcc catgggctac ctcatctccg
     4081 ggcccgtggg cacgggcaag agcttcctgg tcaccgcctt cgccggggag atcgggatcc
     4141 ccatggtcaa gttcaagaac ttccggtcca agtggcaggg ggttacagag tccaacctgg
     4201 agaaggtcct gaacatactc aaggccatgg cccccgtggc ggtgatgatc gacgaggccg
     4261 acgccttttt gggggaccgc aaccaggagg gcgactcggg cacctccaac cgcatcttcg
     4321 cccagatcgc ctcgttcatg ggcaacaccg agtaccgggg ccggatcatc tggttcctga
     4381 tcacctgccg gccggacctc ctgcccatcg acctcaagcg ccagggccgg gcggaggagc
     4441 acctggccct cttctacccc gacacggacg cggagaagga ggccctcttc gacaccctgg
     4501 tgcgcaagct cgacctgtcc atccggaagt tccccgtggg ggacctcctc aggcgcttca
     4561 agtacgagtt ctccggggcg gacctggagg ccgtcctgat ccgggccaag ttccgggccg
     4621 ccatggacgg ccgatccttc gtgacccggg aggacatgga cgagatcctg gccgacttcg
     4681 tgccccccgc ctacccccac gagatcgagc tccagaacct ggtggcggtc ctggaatgca
     4741 ccagcaagga gatggtcccc aagcgcttcc agaacctgga ccggaccaag ctggtgcggg
     4801 acatccggga gatcaaggaa ctcctgggag agcgagagta gggggagcgc gttcgggaaa
     4861 actccacagg aagcgccgaa cgtggtatcc caatggggca ctgcggaatc ccggggtttc
     4921 gctacgtcct acgacgcttc gcgtcgtttg tcgagcggaa gtgattatcg tgtgcgccat
     4981 cgcgcaccac gacatcgcct tatcgcccaa ggatgggcgc ttgcccatga atccagaatc
     5041 gctttgcgat tctgaatcgt cagggacgac gcgccccctt cgggtttagt ctggttcggc
     5101 gcttcctggg cggtcccgcg ggtgcgccgc ctattcccgg tctgtttgac cgggggagaa
     5161 aggggcagcg gtcgattccg tcgcgaggcc gcggcggcgt tctttaacgc ggctccccct
     5221 cgctacgaag gccgcgcgta ccgcgcggcg cttctccgct accccttcgt aggtttattc
     5281 ccagaaactc gacgaagccg ccaggcttcg tcgccgtgag gccggcaccc aggatgggtg
     5341 ccgaattgcc ggccagaatt gcacaggcaa ttctggatca tcacggatga tgcggcctca
     5401 cgcgcaccct cgcacttgtt aacggacggc gggccggacg tcctcatcga atccccggaa
     5461 cgcaaggcgg gctgcaaggg tcagttcgaa cagcgccgtg ggcacattca gggcgaagta
     5521 gatcggtgtg gccattttga tgaattcgag catcagcatc acggtcgcga gtatcgtcag
     5581 cgcagcaccg gtgaatcccc aaaccgacag ccatctcggc acgagcctgt ccttgaaaag
     5641 tccgtagcag aggatcaacc cgccggtcat ccacggtaaa accattccta tgtgattcat
     5701 gtggtcccta ccctgcctta gcagctctcc caaggtcctg tagtatgacg ggtcggaagg
     5761 tgcctcggcc gcgaaacgcc ggctaagcat taaaagcagc agaagggatc ctatcccgac
     5821 atagagaaac gcggctccga cgatcctgaa cacgagatac acggtcgcca agacgtcgcg
     5881 tcgctttcgg agtacctgat taaacaatac cgcgatgtac acgtatatcg tagccataac
     5941 ggcctggaaa aataccgccg tgagaaccca cgttcggatc gacggcagtt tctccaggta
     6001 atccggatat tccaacgccg gcaccgtgtt caatatcccg catgcgatcg cggaaacgag
     6061 caatatcccc agatatcgcg ccgtcctctt gtcatccatc acgatactcc ttgggtcgaa
     6121 ttcgattcca ccggctttca tgagtccgcc cgctcgtccg gatactggaa gacctcttca
     6181 aggggaacgc cgaaaacccg ggcaatctgg aacgccgtct cgagagtcgg ggaataccgc
     6241 ccttgttcga tcgcgatgat cgtctggcgg gtcaggctca cgcgatccgc cagttcggcc
     6301 tgcgtcatct cgccggcggc gaacctcagc gaccggatgt tgttcgtaac cagggtgcgc
     6361 gcggccacgg ctagaacccc cggcgatagg cgatcagctt ggacgccgac gaaacgagag
     6421 cccccgcgac gaacgagagg tacagggcgt tggcgatcca gaacggatca tatcggagca
     6481 tagccagggc cagggccccg agcgcacccc cggacgagac gtagtacccg acgagctcgc
     6541 cccgcctgtt gatttcggtg tccctctggt cgctcctgtt gacctcggct tcggatccgt
     6601 ctcccttgat ccgcgcggag acggagcccg ccaccgccac cagaatcgct ccaaggatcg
     6661 acatcacgat caccgagccg atcgcgatga gcaacggccc ttggtacgag acctggtcca
     6721 ccggcacgga ctccatccgc ttgagcacga gcgctacgta gacagccgcc accgccgccg
     6781 aaacgaccgc gttaacccag gtgatcttct cctcgaacgg catcacagcc tccttgtttt
     6841 ggttcctatc gacctatgaa caccaacccg acatcgcgag gcgccgcagg acgcctcggc
     6901 ctgccctttt acgcagatga ttcgcccctt gtgccgaaat cggccatgtc aagttttctt
     6961 tacttgatgt aatgtaaatc ataacattct ttatgtcaag tatattttac acatatcaag
     7021 atcaacaatt caatctatcg ggagtgcgcg gcggaacctg gcagcccaaa cggccggcgt
     7081 tgactccggg ggacggacgg cctagagtag cgccatggat tctatcgtag tcggactgaa
     7141 gtccgccctc gccgccttct tcggcttcaa ggcctacgtc atccttccgg cattcatgct
     7201 ggtcctggcc ctggcggccc ggatgaggcc gaaggaggcg ctcttcgcct cgatcaagat
     7261 cggcgcgggc ttttccggcg tcttcatcgt cttcagcttc ttcgtgaccc agatacgacc
     7321 ggcgatcgag gccgtcatcg ccctgcgcgg cctcaattac ccggtggcgg acgtgggctg
     7381 gccgcccctg gcggccatca cttggtcctc ccccctcacc cccctgctca tccccctggt
     7441 cctcgtcctg aacctggtca tgatcgcggc gcggagcacc aagaccctct acatcgacct
     7501 gtggaactac tggcatttcg ccctgatcgg ggtgctggtt cggaacgcga cgggcagcct
     7561 ggtcctgggc ctggccgcgg gcctcctcct ggcgctctac accatcaaga ccgccgaatg
     7621 gtccgctccg tacgtgcggc gggagacggg actggaggga ctggccgtca gccccgtgtc
     7681 ggtcgcgggg ctgcttccct acgcggtggc gctgaaccgg gccttcgacg cgatcccggg
     7741 cctgcgccgg ctttcctggg atccctcccg gacaggggcc gagggctcgg ggccgcgcct
     7801 cctgcaggaa cccatggtca tagggatcct ggtcgggacg ggcctttccc tgctggccgg
     7861 gtacgacctc aagggcgtcc tggagacctc cgtaaacatc gcggccgtca tgttcctcct
     7921 gcccaagtgc ggagccctca tcggggaagg catgggggcc gtatccctgg ctttcaagct
     7981 cctcgtggag aggcgcttcc ggtccctgcg cggactttcc atcgccgtgg acaccggaat
     8041 cctcatgacc aacccctccg tcatggcgac ggggctcatc ctgataccgg tgtccctgct
     8101 ggtcgccttc ctcctgcccg gcaaccgggt cctgcccctg ggggacctgc ccaacctcat
     8161 ctcgatcatg tccctgtgca ccctggtcat gggaggcaac gtcctgcgat ccgtgctggc
     8221 gggcgtaccc gtcatcgcca gcttcctgct catagcctcc gacatggccc ccctgtacac
     8281 cgagcaggcc gcggctgcgg ggatgaacct ggacctcggg ggccgcgagc tcacggcctt
     8341 cacggacggg ggcaaccagc tccgctactg gctctacggg ctgttccagg gaagggccgc
     8401 ggcctgggcg gcggtccccc tcgtgctggg cctgctgtgg ctggcagggc gggaacacaa
     8461 gaagatggcc gacctcgaac cgccgaggtg aacgccccgg cccgaaaatc gggccggtac
     8521 cgggcttccg cggttttctc ggcgatcatc cggcatttct cggcgttgac cgtcgaacgc
     8581 cgatggaggc gaccatgcgt cattcctcgt tcccccgttc tctggcctgc attctcgccc
     8641 tgaccctcgg cctgtccggg aacctggccg cgcaggagga gttcatccgc ctgccgggag
     8701 gcggcttcct gatgggctcc cccgtcacgg agaagcagcg gaatccggac gagggacctc
     8761 aacatccggt caaggtcgcg cccttcacga tgggccggta cgaggtgacc caggccgagt
     8821 ggacggcgct gatggaaaac aatccgagcc ggttccgggg cgaccggctg cccgtcgagg
     8881 gggtgagctg gtacgaggcc ctggtgtact gcaacaagcg cagcatcgcg gaaggtctgg
     8941 atccttgcta taccatacag ggaagcgcgg atccggcgga ctgggggagc gtgccgacga
     9001 gttcgaacgg cgcctggaat gccgtcgtct gcgatttttc cgcagacggc taccgtctgc
     9061 ccaccgaggc tgaatgggag tacgcgtgcc gggcgaggac ggaatcggcc acggccttcg
     9121 gagactccct gggtccggac cgggcgaact tcgacgggag ccggccgtac aattctgaga
     9181 tcctcgcgga acccctgcgc cgcacgacgc ccgtggggag ctacccggcg aacccctggg
     9241 gtttccacga catgcacggc aacgtctggg aatggtgctg ggatcactac gccgacaaat
     9301 actacgcccg atccccggat acggatcccc ggggggcgga gggcgggcgg taccgggtcc
     9361 tgcgcggcgg cgcctggggc aatttcggct accggctgcg gtccgccgcc cggcgcgacg
     9421 aggacccctt ctaccgggac gccgacaacg gattccggct cgtacggggg agatagccgg
     9481 gccggcccta gcaggctgtt gacaaaggcg atggcgcctt tgtcaacagc ttcctcaata
     9541 ccgcaattgc gcagaaaagc gagagctttt cgagcaattg catagcaggg tgctgacaag
     9601 cttcgagttt gtcagcaccc tgctagacga actggtcgaa gaggacaggg ttcccgtcgg
     9661 gatcctccac cgtgaagctg gcgggtcccg aggtcgactc gtcggcctcg gtgacgagcc
     9721 ggacaccctt ccgcttgagc tccttctgga tctcccggat gtccgtgaag gagggcagcg
     9781 cctgggcgtc ctggtcccag ccgggattga aggtcagggt gttcctgggg atcatgccct
     9841 ggaagaggcc caggatgcag accccgttct tgaggatcag ccagccctgg gccgcgtccc
     9901 cgccgaaggc ggagaatccc agcttctcgt agaaggccct cgaggcctcc acattcttca
     9961 cggcaaggct gacggaaaag gctccgaggt ccatggcggc tcctttcgca ccggggaggt
    10021 gctggaggag aagatactga aaacgggagg cggaagagtc aaaaaggaaa cccaaggacg
    10081 ttcctcgaag gctgcacatg cgaaggataa tccctcgcag tagtccgaat ccaatcgccc
    10141 ctccccttgc cctcctcccc tccccgcccc atactttccc catggaacgc accagcctca
    10201 cgcatcccct gattgtcgac tccgtggaga cacccggggg tgggcggatc ggcctgacct
    10261 tctgcccggg gaaaaagcag gcccgggccg cctcgggatc ctggaaccgg gacctggacg
    10321 cggacctgga cgcgatccgg gcctggggag cggaggtcct ggtcagcctc atcgagcccg
    10381 cggagtacca acggctcggg gtggaggacc tgccggagaa ggcccgggcc cggatggccc
    10441 acctggccct gcccgtcccg gactacgggc t
//
LOCUS       DAOTEI010000125        10454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4488_length_10454_cov_6.322820, whole genome shotgun sequence.
ACCESSION   DAOTEI010000125 DAOTEI010000000
VERSION     DAOTEI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4488_length_10454_cov_6.322820"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="P5313_11765"
     CDS             complement(<1..718)
                     /locus_tag="P5313_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY81085.1"
                     /translation="MKLAVITGFLGKTKDRFHEYNEALDLDAKFKLLASIPGYSGVEV
                     VHPYEVPSAAELKTLLAKHRLSVAAINVNVKAEPEFRSGGLTSADPGVRAKAVRFLKE
                     AKDYAAAVGADKVTCCPLGDGYEFSFQYDYAASWRHLVDAFAEAAAHRPEVTLFVEYK
                     PSETRGRCFVDSAAKTVCLVRDAGSKGLGVTLDFGHSVYGGENPAEAAALVHGSGIPL
                     YVHINDNDGKWDWDYFCGTKH"
     gene            complement(729..1679)
                     /locus_tag="P5313_11770"
     CDS             complement(729..1679)
                     /locus_tag="P5313_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY81086.1"
                     /translation="MSLPPAKVPTRRAIMDRLVEAAKEDPSFVVLDSDIGYSTYSYLF
                     GDAFPDRYFNLGIAELGTMAAAAGLAADGRTVFVCGYGVFLTMRALEAVRSFVCYPHL
                     NVKILSSHAGVTAAIDGVTHQATEDLAFMSTLPNMTVLAPCDSSSARAAFDLARNTPG
                     PVYVRLMRDPLKEVYAPETTFRVGGSHTPRRGKDVTIACLGDMVIQAIEASELLAAEG
                     ISAEVLDLYSLKPWDEAGLAESLDRTGALLAAENHQRRNGLGYELAAWCMRNGRSVPF
                     SQAGLADTFAESGAYGKTLEKFGMDAAALARAARELVSRK"
     gene            complement(1676..2527)
                     /locus_tag="P5313_11775"
     CDS             complement(1676..2527)
                     /locus_tag="P5313_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY81087.1"
                     /translation="MTTSNARSLDERAAFLEMKAREVRKAIVEMVHSAQSGHCGGSLS
                     AADFVTALYFDLMDIDPAEPRKPGRDRFVLSKGHACPVWYAALALRGYFPAEELSTLR
                     RNDSRLQGHPVATYLPGIDATSGSLGLGFGQALGMALDARLSGRKNTVYAVLGDGELN
                     EGVVWETAAQAAKYRLDNLVAVVDRNNLQNDGRADVIMPMEPIDKKFEAFNWRVLEMD
                     GHDMRQVLSTLEKARERSGRPTCVIARTIKGKGVSFMEDKREWHGKPPSDEERRRACE
                     EIEGRTP"
     gene            complement(2524..3489)
                     /locus_tag="P5313_11780"
     CDS             complement(2524..3489)
                     /locus_tag="P5313_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY81088.1"
                     /translation="MYGVSPAYFFSSFGPGFRPSDILDALPGLRGLGYESFQPEAFRA
                     EAAEAWTGGDSERIAARARDLGLSVGPFVAHWLGAGFSTAESLGRRGLPEGTDRALEI
                     AAALGGASVFAVPLPALALSRQGGPARGADSAIGPIRAGLRGALIEKLGRLAEAVRKA
                     GFRPALELLPGNALGGGAEFRGLLSVPGFGDLGLVLDTGHFHAMGEEVPVLPESLGGA
                     IAATHLCDNDGIENLSLAPGAGTIPFRPLLAALAASGYPGGLDIEIVCPPGDVAREYR
                     KALDTLRSLDPSDPAREGPAAEAAERRGGMNTREPGARTLERSPA"
     gene            3613..4281
                     /locus_tag="P5313_11785"
     CDS             3613..4281
                     /locus_tag="P5313_11785"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY81089.1"
                     /translation="MRESRFEDLAEQVYRALREMILTGELVPGQKLVQEALSERLGVS
                     RTPLLAAFSKLEKELLVELVPRRGAYVKRLSPLELLHLYDVRLRLEPLGAAEAARNLD
                     AAGRAELEKAFSAYRDCVTDRDAARVRVADFEFHGILMRLSRNSILDRILSSFAIVLA
                     SNQRGLMKPMEKSLAEHEALVEAILRGDEGRAESIMHAHLAEARDAAAALAAGAAGPA
                     AGGI"
     gene            4283..4930
                     /locus_tag="P5313_11790"
     CDS             4283..4930
                     /locus_tag="P5313_11790"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY81090.1"
                     /translation="MPRIQRTVVSSAVYDAVKEMLFDGTWVPGDRIDRKALAVRLGVS
                     PTPVNDALHRLTGEGLVESRIRDGFFVPDYSDEDLADLFAVRAGIEGVAARLCAEEAL
                     PGEVEKLCALFDPFSRGIPPERTEEYLRADKSFHAGLLAASGSARLREVERFFGHVFR
                     SYEHGLVRSPGETLPEHRRIIEALRSRDGREAQAAMSDHLLRSRRALMDRARSSG"
     gene            5009..6991
                     /locus_tag="P5313_11795"
     CDS             5009..6991
                     /locus_tag="P5313_11795"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY81091.1"
                     /translation="MKKLGLLFLIVLFLGGCALAPHRDVKAAVLDLRASENLVPLTGE
                     WNAAVRGRISAAPAPLRSLLPVPTAGEPETWTLTTLFRAGGEYALLLPFPAEITGVYL
                     DSRTVWERSAGGAGPARPRILELPPDASGVLRVEVPAGGRSPGRPTLAATPFILGSSE
                     AVEDYRSLVALLAGVKVGAFGGALLVSLLFALISRRGKGFLSLAIYLAGTLVYFLVRD
                     ASVLGWIPLDSYFLDRLFSSAWSLQTSALAVLALALCDRALPAPVRPALTVPPLLLAG
                     AAFLPLPILLPVHAAALVWTALSFAALIGWSAFRSLRGDRRAVPVLLGGILLAAAYGA
                     RLAVPDTLLLARLLEPAGAGAFTVLASLTMIRKVGDAFEEAENLSAYVDSVSTSVKRF
                     IPAEFMDYLQKTDVTDLKLGDHVEKAMTIFFSDIRGFTQLSESLTEEQTFAFINSYLA
                     RMVPVIKERGGFVDKYVGDAIMALFPDTGGADQALRAALEMQSRVVEYNRHRASVGYR
                     AIEMGIGIHTGTLMLGVVGVQDRMENTVISDAVNLASRLQAIAKAFNIGVVVSEQSFK
                     SLEDPTLFKYRFIGKVRVKGKAAPVSCFEIFGDTQDEAFDEQKMKASTYFEQGMLRYY
                     QKDFLAGFHYFQKALEFLPQDGASKFYMEVCWRKKD"
     gene            7007..9007
                     /locus_tag="P5313_11800"
     CDS             7007..9007
                     /locus_tag="P5313_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRY81092.1"
                     /translation="MGLSFGVDWYPEQWERSLWESDADRMKAFGVRTVRMMEFAWALV
                     EPEPGRFDFSLFDEAIRVLADRGISVILGTPTATLPAWLAGDPSGVLQTHPSGRVRDF
                     GARREGCWNAPAYREASRRLVRAIAARYGKRPEVEGFQIDNELGHEGSDRCVCPHCAR
                     EWRAWLARRYGDVKALNEAWGTVFWGRTISDFSQVPVPRIQPATRHNPALLLDYDRFL
                     SDTAAAFVNEQVEVLRAASTPEKWITTNLFPVPFSLSLDMEDVTRGMDFASWDNYPVW
                     GEDEAPLPYFFQSFLMTYVRGLKKDRAFTVMEQMSGIQAHVCLGHLPSERQVALWTAQ
                     AVARGAERIVYFRWRTAAFAQEQLCYGLLDTDNRETERMRVLGENLTRAETAFSDLAS
                     EPVESPACLVYSKDDARLLREQYLSLGLYHRANDWMQAGLDAELAKWFAPFVALNVNA
                     DVKSVRSVDLDRYKLVSLPLYQMADPAFVERLAAWVDRGGVLVLSYRAGARDLSNRNV
                     SEPLPGLFRALAGVRIPRFEALGKTKARLRVGRLPIPAHGEVWADIVEPETARPAAFW
                     TDRKKFYRGAPCVTVNSLGKGTVWYVGTSPDPAATFLLYRRIFKSAGIAPRFAGPGVE
                     IVDRRLSDGSEVSVILNHSPRRRRVLGRTLEPWGWAVVPKRG"
     gene            9018..9542
                     /locus_tag="P5313_11805"
     CDS             9018..9542
                     /locus_tag="P5313_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81093.1"
                     /translation="MDAADWDARGRELTDRGDLEGAEAAFRSALELDPGSPDLLYNLG
                     TVLRDRGLRGEARSAFLAALDAAPPRADALTALGLLAWEEGDGPGAETLYREALRLDP
                     AYPEAWNDLGVVLFVSARYREAREAFERAVALRDDYADAWFNLRDACSELGDRRGARA
                     ADRRYRALKGGARV"
     gene            9693..>10454
                     /locus_tag="P5313_11810"
     CDS             9693..>10454
                     /locus_tag="P5313_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HRY81094.1"
                     /translation="MKRRFRTLAAIALIAALFLGSCATKFDQFVDQPLKITFIETSDI
                     HGAIFPYDFITGKPLATSMAQVAGLVKEKRAAGEVVLLEDGDSLQGQPTVYYYNFEKT
                     GVPHIWSDVLNYLAYDAVTVGNHDIEAGHPVYDKMVKELKMPFICANVVDEKTGEPYF
                     KPYAVVTRQGVKIAILGLTEPKITEQLPRAFWSGMDILDMVETAKKWVPIILEKEKPD
                     LLVGLFHAGVDYTYGGQTADTPKNESGSQLVAERVP"
BASE COUNT         1583 a         3626 c         3560 g         1685 t
ORIGIN      
        1 agtgcttggt gccgcagaaa tagtcccagt cccacttgcc gtcgttgtcg ttgatgtgta
       61 cgtacagggg tatgccgctt ccgtgcacca gggcggcggc ctccgcgggg ttctcccccc
      121 cgtagaccga gtgcccgaag tccagggtca cccccaatcc cttggatccc gcgtcccgga
      181 ccaggcagac cgtcttggcc gcgctgtcca cgaaacaacg tccccgggtc tccgagggct
      241 tgtactccac gaagagggtc acttcgggcc tgtgggccgc ggcctcggcg aaggcgtcca
      301 cgaggtgccg ccaggaggcg gcgtagtcgt actggaacga gaattcgtag ccgtccccca
      361 ggggacagca ggtcaccttg tccgccccta ccgcggcggc gtagtccttg gcctccttga
      421 ggaaccggac cgccttggcc cgtaccccgg gatccgcgga ggtcaagccc ccggagcgga
      481 actcgggctc cgccttgacg ttcacgttga tcgccgctac ggaaagcctg tgcttggcca
      541 ggagggtctt gagctccgcg gcggagggca cctcgtaggg gtggaccacc tcgaccccgg
      601 aatagccggg tatggaggcc agcagcttga acttcgcgtc caggtccagg gcctcgttgt
      661 attcgtggaa ccggtccttg gtcttgccca ggaatccggt gatgacggcc agcttcatgc
      721 ggtcctcctc attttctcga gactagttca cgggccgccc gggcgagggc ggcggcgtcc
      781 atgccgaatt tctccagggt cttcccgtag gccccgctct ccgcgaaggt gtccgccagg
      841 cccgcctggg agaagggcac ggaccggccg ttccgcatgc accaggccgc cagctcgtag
      901 cccagaccgt tccgacgctg atggttttcc gccgccagga gggctccggt ccggtccagg
      961 gactccgcaa gaccggcctc gtcccagggc ttcagggagt ataggtccag gacctccgcg
     1021 gagatcccct cggccgccag gagttcggag gcctcgatcg cctggatgac catgtctccc
     1081 aggcaggcga tggtcacgtc cttgccgcgc cggggggtgt ggcttcctcc cacccggaag
     1141 gtggtttcgg gggcgtagac ctccttcagg gggtcccgca tgaggcggac atacacgggt
     1201 cccggtgtgt tccgtgccag gtcgaaggcg gcccgggcgc tggagctgtc gcagggggcc
     1261 aggacggtca tgttcggcag ggtggacatg aaggccaggt cctcggtggc ctggtgggtc
     1321 accccgtcga tggcggcggt cacccccgcg tggctggaga ggatcttcac gttgagatga
     1381 ggatagcaga cgaaggaccg caccgcctcc agggcccgca tggtcaggaa gaccccgtag
     1441 ccgcacacga agacggtccg gccgtccgcg gccaggcccg cggcggcggc catggtcccg
     1501 agctccgcga tgcccaggtt gaagtaccgg tccgggaacg cgtcgccgaa gaggtaggaa
     1561 tacgtggagt atccgatgtc cgagtccagg accacgaagc tcgggtcttc cttcgccgcc
     1621 tccacgaggc ggtccatgat cgcgcgccgg gtgggcacct tggcgggggg caggctcacg
     1681 gggtcctccc ttcgatctcc tcgcaggccc gacggcgctc ctcgtccgag ggcggtttgc
     1741 catgccactc gcgtttgtcc tccatgaagc tcactccctt tcccttgatc gtgcgggcga
     1801 tcacgcaggt cggacggccg ctgcgttccc gggccttttc cagggtcgag aggacctgcc
     1861 gcatgtcgtg cccgtccatc tcgaggaccc gccagttgaa ggcctcgaac ttcttgtcga
     1921 tgggctccat gggcatgatc acgtcggccc ggccgtcgtt ctgcaggttg ttccggtcca
     1981 cgacggccac caggttgtcc aggcggtact tggccgcctg ggccgcggtc tcccagacca
     2041 ctccctcgtt cagttccccg tcccccagca cggcgtatac cgtgttcttc cggcccgaga
     2101 gccgggcgtc cagggccatg cccagggcct gtccgaagcc gaggcccagg gatcccgagg
     2161 tggcgtcgat cccgggaagg taggtcgcca cggggtgacc ctggaggcgg ctgtcgttcc
     2221 gccgcagggt ggaaagctcc tccgccggga aatagccccg gagggccaga gcggcgtacc
     2281 agaccgggca ggcatgtccc ttggacagga cgaagcgatc ccggccgggc ttgcggggtt
     2341 cggcggggtc gatgtccatg aggtcgaagt agagggccgt cacgaagtcc gccgcggaga
     2401 ggcttcctcc gcagtgcccg gactgggcgg agtggaccat ctccacgatg gccttgcgca
     2461 cctcccgggc tttcatctcc aggaaggcag cccgctcatc caaagagcgg gcgttcgaag
     2521 tcgtcatgcg gggctccttt ccagggtgcg ggcgccgggc tcgcgggtgt tcatgccgcc
     2581 gcggcgttcc gccgcctcgg ccgccggacc ctcccgggcc gggtcgctcg ggtccaggga
     2641 ccggagggtg tccagggctt tacggtattc ccgggccacg tccccggggg ggcatacgat
     2701 ctctatgtcc agcccgcccg ggtatcctga ggcggccagg gcggccagga ggggtcggaa
     2761 ggggatggtc ccggccccgg gggccaggga gaggttctcg atcccgtcgt tgtcgcaaag
     2821 gtgggtggcc gcgatcgccc cgcccaggga ttccggcagg accgggacct cctcccccat
     2881 ggcgtggaag tggccggtat ccagcaccag gcccaggtcc ccgaagccgg ggacggatag
     2941 gaggccgcgg aactcggcgc cgcctcccag ggcgttgccc ggaaggagtt ccagggccgg
     3001 gcggaagccc gccttgcgga cggcttcggc caggcggccc agcttctcga tcagggcccc
     3061 ccggagaccg gcacggatcg ggccgatcgc ggagtcggcg ccgcgggcgg gtccgccctg
     3121 gcgggacagg gccagggcgg gcaggggcac cgcgaacacg gacgctcctc ccagggccgc
     3181 cgcgatctcc agggcccggt ccgttccctc cgggagcccc cggcgtccca gggactcggc
     3241 ggtcgagaat cccgccccca gccagtgggc cacgaaggga ccgaccgaga gccccaggtc
     3301 gcgagcccgg gcggcgatcc tctcggagtc ccccccggtc caagcttccg cggcctccgc
     3361 ccggaaggct tccggctgga aggattcgta cccgaggccc cgaagccccg gcagggcatc
     3421 caggatgtcc gagggccgga agcccgggcc gaaggaggag aagaagtatg cgggggaaac
     3481 tccgtacata cgacctgcct aaaaatattg tatacaaaac atagaccgac tccgggctcc
     3541 tgtcaagggc ggaacgatcg gtttgtatac aaaatcattg accgggtcac ccagcgcggc
     3601 tagaatgggg ccatgcgaga atcccgcttc gaagacctcg cggaacaggt gtaccgggct
     3661 ttgagggaaa tgatcctgac cggggaactc gtgcccggcc agaagctggt gcaggaagcc
     3721 ctttccgagc gtctgggtgt atcccgaacc cccctcctgg ccgccttctc gaagctggag
     3781 aaggagctcc tggtggagct ggtcccccgc cggggggcct acgtcaagcg cctttcgccc
     3841 ctggaactcc ttcacctcta cgacgtccgt ctgcgcctgg aacccctcgg ggcggccgag
     3901 gcggcccgca acctggacgc ggcggggcgg gcggaactgg agaaggcttt ttccgcctac
     3961 cgcgactgcg tgaccgaccg ggacgcggcc cgggtccggg tggcggactt cgagttccac
     4021 ggcatcctga tgcgcctgag ccgcaactcg atcctggacc gcatcctctc gtccttcgcc
     4081 atcgtgctgg ccagcaacca gaggggcctc atgaaaccca tggagaaaag cctggccgag
     4141 cacgaggccc tggtggaagc catcctgcgc ggggacgagg ggcgggcgga atcgatcatg
     4201 cacgcccacc tcgcggaggc ccgggacgcc gcggcggccc tggccgcggg cgcggccgga
     4261 ccggcggcgg gagggatcta ggatgccgcg catccaacgg accgtggtct cctcggcggt
     4321 gtacgacgcc gtaaaggaaa tgctcttcga cgggacctgg gttcccgggg accgcatcga
     4381 ccgcaaggcc cttgcggtcc gcctgggggt gagcccgacc ccggtcaacg acgccctcca
     4441 ccgcctcacg ggggagggcc tggtggaatc ccggatccgg gacggatttt tcgtacccga
     4501 ctattcggat gaggacctgg cggacctgtt cgccgtccgc gcgggcatcg agggtgtcgc
     4561 ggcccggctc tgcgcggagg aggcccttcc cggggaggtc gagaaactct gcgccctctt
     4621 cgatcccttc tcccggggga taccgccgga aaggaccgag gagtatcttc gggcggacaa
     4681 gtccttccac gcgggcctcc tggcggcgtc cggaagcgcc cggctccggg aggtggagcg
     4741 gttcttcggc cacgtcttcc gcagctacga gcacggcctg gtccggtccc ccggggaaac
     4801 cctgcccgag caccggcgga tcatcgaggc cctgagatcc cgggacggac gggaggccca
     4861 ggcggccatg tcggaccatc ttctccgatc ccgccgggcc ctgatggacc gggcccggtc
     4921 gagcggctag cccggaaccc ggtttcccgg cgcggacgac gccatcgcag tggcggagct
     4981 cggcgatccg ggctatagta ggatcgatat gaaaaagctt ggtctcctgt tcctcatcgt
     5041 cctcttcctg ggcggctgcg ccctggcgcc gcatcgggac gtgaaggcgg cggtcctgga
     5101 cctccgggcc tcggaaaacc tggtccccct cacgggagaa tggaacgcgg ccgtccgagg
     5161 ccggatctcc gcggcccccg cacccctgcg ttccctcctg ccggttccga cggccgggga
     5221 acccgagacc tggaccctga cgaccctttt ccgggccgga ggcgagtacg ccctcctttt
     5281 gccctttccc gcggaaatca ccggggtgta cctggattcc cggacggtct gggagcgctc
     5341 cgcgggggga gccggcccgg cccggccccg gatcctcgag ctccctccgg acgcatccgg
     5401 ggtactccgg gtggaggtcc ccgcaggcgg tcgatccccc ggccgcccga ccctcgcggc
     5461 gacgccgttc atcctgggga gttcggaagc cgtggaggac taccggagcc tggtcgccct
     5521 cctcgcgggg gtcaaggtag gggccttcgg cggggctctc cttgtgtccc tgctcttcgc
     5581 cctgatcagc cgccggggca agggcttcct ctccctggcg atctacctcg cgggcaccct
     5641 ggtctacttc ctggtccggg acgcctccgt cctgggctgg atacccttgg actcgtactt
     5701 cctggaccgg ctcttttcct cggcctggtc cctccagacc tcggccctgg ccgtcctggc
     5761 cctggccctc tgcgaccggg ccctcccggc cccagtccgc ccggccctga cggttccgcc
     5821 cctcctgctg gccggggcgg ccttcctgcc cctgccgatc ctgcttcccg tgcacgccgc
     5881 cgccctggtc tggacggccc tgtccttcgc ggccctgatc gggtggtccg ccttccgttc
     5941 cctccggggc gaccgccgcg ccgtacccgt cctgctgggc ggaatcctgc tggccgcggc
     6001 ctacggggcc cggctggcgg tgccggatac cctgctcctg gcccggctcc tggaacccgc
     6061 gggggcgggg gcgttcacgg tcctggcctc cctgaccatg atccgcaagg tgggggacgc
     6121 cttcgaggag gcggaaaacc tgtccgccta cgtggacagc gtgtccacct cggtcaagcg
     6181 attcatcccc gcggagttca tggactacct ccagaagacg gacgtgacgg acctcaagct
     6241 cggggaccac gtggagaagg cgatgaccat cttcttctcc gacatccggg gcttcaccca
     6301 gctctcggag tccctgacgg aggagcagac cttcgccttc atcaattcct acctggcccg
     6361 gatggtcccc gtcatcaagg agcggggcgg cttcgtggac aagtacgtcg gggacgcgat
     6421 catggccctg ttcccggaca cgggcggggc cgaccaggcg ctgcgtgcgg ccctggagat
     6481 gcagagccgg gtggtcgagt acaaccgcca ccgggccagc gtgggctacc gggccatcga
     6541 gatggggatc ggcatccaca cggggaccct gatgctgggg gtggtgggag tccaggaccg
     6601 gatggagaac accgtcatct ccgacgcggt gaacctcgct tcccgtctcc aggccatcgc
     6661 caaggccttc aacatcgggg tggtggtcag cgagcagtcc ttcaagtccc tggaggatcc
     6721 caccctcttc aagtaccgct tcatcggcaa ggtccgggtc aagggcaagg ccgccccggt
     6781 gtcctgcttc gaaatcttcg gggacaccca ggatgaggcc ttcgacgagc agaagatgaa
     6841 ggccagcacc tatttcgagc agggaatgct ccggtactac cagaaggact tcctggcggg
     6901 cttccactac ttccagaagg ccctggagtt ccttccccag gacggggcgt ccaaattcta
     6961 catggaagtg tgctggcgga agaaggatta gcggcccgga ggaagcatgg gactgagctt
     7021 cggagtcgac tggtatcccg agcaatggga aaggtcgctg tgggaatccg acgcggaccg
     7081 gatgaaggcc ttcggcgtcc ggacggtgcg catgatggaa ttcgcctggg ccctggtgga
     7141 gcccgagccc gggcgcttcg atttttctct cttcgacgag gcgatccggg tgctcgcgga
     7201 ccgcgggatc tccgtaatcc tgggaacccc cacggccacc cttccggcct ggctggccgg
     7261 cgacccctcc ggagtcctcc agacccatcc gtcggggcgg gtgcgcgact tcggggcccg
     7321 ccgggagggg tgctggaacg cccccgccta ccgggaggcc tcccgacgcc tggtacgggc
     7381 gatcgccgct cggtacggga agcggccgga ggtggagggc ttccagatcg acaacgagct
     7441 gggccacgag ggctcggacc gctgcgtctg cccccactgc gcccgggaat ggcgcgcctg
     7501 gctggcccgg cgctacggcg acgtgaaggc cctgaacgag gcctggggaa cggtgttctg
     7561 gggccggacg atctcggact tctcccaggt gcccgtaccc cggatccagc cggccacccg
     7621 gcacaacccc gccctgctcc tggactacga ccgcttcctc tcggataccg ccgcggcctt
     7681 cgtgaacgag caggtcgagg tcctccgggc cgcctcgacc ccggagaagt ggatcaccac
     7741 gaacctcttc cccgttccct tctccctgtc cctggacatg gaggatgtca cccggggcat
     7801 ggacttcgcc tcctgggaca actacccggt ctggggggag gacgaggccc ccctgccgta
     7861 cttcttccag tccttcctga tgacctacgt ccggggcttg aagaaggacc gggcctttac
     7921 ggtcatggag cagatgagcg ggatccaggc ccacgtctgc ctgggccacc tcccgtcgga
     7981 acggcaggtg gccctgtgga ccgcccaggc cgtcgcccgg ggggccgaac ggatcgtcta
     8041 cttccgctgg cgcaccgccg ccttcgccca ggaacagctc tgctacggcc ttttggacac
     8101 ggacaaccgg gagaccgagc ggatgcgggt cctgggggag aacctcaccc gcgccgagac
     8161 ggccttctcg gacctcgcct cggagcccgt ggagagcccc gcctgcctgg tctactccaa
     8221 ggacgacgcc cgcctccttc gggaacagta tctttccctg gggctttacc accgcgccaa
     8281 cgactggatg caggcgggcc tggacgcgga gctggcaaag tggttcgccc ccttcgtggc
     8341 cctgaacgtc aacgcggacg tcaagagcgt ccggtccgtg gacctcgacc ggtacaagct
     8401 cgtctccctg cccctctacc agatggccga ccccgccttc gtggagcgcc tggccgcctg
     8461 ggtggaccgg ggcggggtcc tggtcctgtc ctaccgggcg ggcgcccggg acctctcgaa
     8521 ccgcaatgtt tccgaacccc tgccggggct cttccgcgcg ctcgcggggg tccggatccc
     8581 gcgtttcgag gccctgggca agaccaaggc ccgtctgagg gtcggccgac tgccgatccc
     8641 cgcgcacggg gaggtatggg cggatatcgt ggagcccgag accgcccggc ccgcggcctt
     8701 ctggacggac cggaagaagt tctaccgggg cgccccctgc gtcaccgtga attccctggg
     8761 caagggaacg gtctggtacg taggcaccag tccggacccc gcggcgacct tcctgctata
     8821 ccgccgcata ttcaagtccg cgggcatagc cccccgattc gcggggccgg gtgtggagat
     8881 cgtggaccgt aggctatccg acggctccga ggtttccgtc atcctgaacc acagtccccg
     8941 tcgcaggcgg gtcctgggac ggaccctgga gccctggggc tgggcggtgg tccccaagcg
     9001 gggatagcgg gacctccgtg gacgccgcgg actgggatgc ccggggccgg gagctcacgg
     9061 accgggggga cctggagggg gcggaggcgg ccttccggtc tgccctggaa ctcgatcccg
     9121 ggagcccgga cctcctctat aacctgggca ctgtcctccg ggaccggggc cttcgcgggg
     9181 aagcccggtc cgccttcctg gcggccctgg acgcggcccc tccccgggcg gacgccctta
     9241 cggccctggg cctcctggcc tgggaagagg gggacggccc cggtgcggag accctctatc
     9301 gcgaggccct ccgcctggat cccgcctacc cggaagcctg gaacgacctc ggcgtcgtcc
     9361 tcttcgtctc cgcccggtac cgggaggccc gggaagcctt cgaacgggcc gtcgccctcc
     9421 gggatgacta cgcggacgcc tggttcaacc tccgggacgc ctgctccgaa ctgggggacc
     9481 gccggggggc ccgggccgcg gaccgccgct accgggcctt gaagggcggc gcacgggtat
     9541 gaatcgaagc gcaaccgggg cgggcgatcc ggctccgatt gcgggatccg gcggaggact
     9601 cacatttccc taacaaacac ggctgcccgg gtgtatactg gatcccccgc gcgccgaaga
     9661 gcgacccgcg gggattctaa aaggaggcat ccatgaaacg tcggttccgc actctggcgg
     9721 cgatcgccct gatcgcggcc ttgttcctgg ggtcctgcgc gacgaagttc gaccagttcg
     9781 tcgatcagcc cctcaagatc accttcatcg agacctcgga catccacggg gccatctttc
     9841 cctacgactt catcacagga aagcccctgg ccacctccat ggcccaggtc gcaggcctcg
     9901 tgaaggagaa acgggccgcc ggggaggtcg tgctcctgga ggacggggac tccctccagg
     9961 gccagcccac ggtctactac tacaactttg agaagaccgg ggtcccccac atctggtccg
    10021 atgtcctcaa ctacctggcc tacgacgccg tcacggtggg gaaccacgac atcgaggccg
    10081 gacaccccgt gtacgacaag atggtgaaag agctcaagat gcccttcatc tgcgccaacg
    10141 tggtggacga gaagacgggc gagccctact tcaagcccta cgccgtcgtg acccgccagg
    10201 gcgtgaagat cgccatactg ggcctcacgg agcccaagat caccgagcag ctcccccggg
    10261 ccttctggag cggtatggac atcctggaca tggtggaaac cgcgaagaag tgggtcccga
    10321 tcatcctgga aaaggagaag cccgacctcc tggtcggcct cttccacgcc ggggtggatt
    10381 acacctacgg cggtcagacc gcggacaccc ccaagaacga gagcggcagc cagctggtcg
    10441 ccgagcgcgt cccg
//
LOCUS       DAOTEI010000126        10322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4560_length_10322_cov_5.102464, whole genome shotgun sequence.
ACCESSION   DAOTEI010000126 DAOTEI010000000
VERSION     DAOTEI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4560_length_10322_cov_5.102464"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1297)
                     /locus_tag="P5313_11815"
     CDS             complement(38..1297)
                     /locus_tag="P5313_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRY81095.1"
                     /translation="MSKTSIVGAYNTAFGSFVKKNKETKEVTDLKSIYELIEEAGRGA
                     LADAGIEADRIGGIWVGSCSPSLFANQEHLAPNALEVDPKGLRFKPAYRVEDACASSS
                     AALYSAVYAVEAGRCDAALVIGVEKMNLLDTKGVTHALACSSHWPTEGGMGMSFPALF
                     AEYAKGYRDRYKISDKKLAEMLAAVAALCYRNGLDNPLAHFGKGGPSDKLGLVTAEAI
                     LGLPEDKNPMIAEPLRLHDCSLVTDGAAALVVMRTEEARKRGRPAVEIAGIGHTTERM
                     PIKVRPNMHELMAGKEAARRAFAEAGIASRDIDIAEVHDCFTINQILCTEALGLSADG
                     KAGDDYLAGRFGRDDKVCVNLSGGLKAKGHPVGATGASMHALLYKQLTGHAIGTAPSK
                     GTPKVGAVLNVGGSSVTNCVTVLRVLG"
     gene            complement(1355..2020)
                     /locus_tag="P5313_11820"
     CDS             complement(1355..2020)
                     /locus_tag="P5313_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRY81096.1"
                     /translation="MEYTANKEVIAKRIARIFKDGDVVNLGIGLPTLVGNYVPAGVTI
                     VLQSENGFLGLGPAPAPGREDPALVNAGGGFVTIQPGGAFFDSAMSFAIIRGGHVDAT
                     VLGVLEVDQKGNLANYKIPGKMVPGMGGAMDLTVGAKIVVAATEHFDKSGASKLLRAC
                     KLPLTAAGQVDFVCTDVGFFEVRNGTFVLKEYFKPYTVDYILEKTDADIVVDKDCREV
                     DLG"
     gene            complement(2025..2708)
                     /locus_tag="P5313_11825"
     CDS             complement(2025..2708)
                     /locus_tag="P5313_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRY81097.1"
                     /translation="MVAKPIIAAEKAVELIKEGDTVMVGGFLGCGSPPALIDALEQQG
                     TKNLTLVCNDTAFLNFKTGACTHVAKLVAKKQFRKIVVSHIGTNQETQRQMNEGETEV
                     VLVPQGTLAERIRAAGAGLAGVLTPTGVGTEVQEGKQVLDVDGRKFLLEKALPGNVAL
                     LKAKKADKAGNLVYDKAARNFNPLMAMACETVIVEADEIVEIGQLDPEIVVTPSVLVH
                     YLVRSKKQG"
     gene            complement(2863..4197)
                     /locus_tag="P5313_11830"
     CDS             complement(2863..4197)
                     /locus_tag="P5313_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HRY81098.1"
                     /translation="MIRALGNFFTKIAKKWIPDAFIFAIILTILVFVLGLVIQGKSFI
                     EMTSYWGGGMWSLLGFSMQMVLVLVTGSALALSKPVHRVLKTIASAANTPAKAIAIVA
                     FVMGIGCWLNWGFGLIISAILAKEIAKVTKGIHYPLLVASSYSGFVLWHSGLSASIQL
                     TIAAKNPVMEKVAPGTIIPTGETIFSPQTLIIVLVLLVTVPIINVLMMPKKKEEIVEV
                     DPSIFAEDEKEEVATKSFKGMTPAEKIENSIVLAIIVGVVGLVYAVRYLAKGGSLSLD
                     VVNFIFLVLGLLMHKTPINYVKAVDKSVRNCGGIVLQFPFYAGIMGMMVSSGLAATFS
                     NWFVSISTPKTFQLFTYWSAGLVNMFVPSGGGQWAVQGPIMMPAAQALGVSYARTASA
                     IAWGDAWTNMIQPFWALPLLAVAKLGIKDIMGYTTVIMLWVGIITSLGLLFL"
     gene            complement(4351..5691)
                     /locus_tag="P5313_11835"
     CDS             complement(4351..5691)
                     /locus_tag="P5313_11835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY81099.1"
                     /translation="MKYTILVCDDERNVRVFLRDLLVLEGFEVVEADSGKAALDAART
                     AKPDLCILDLKLPDCNGTDIIPDLKAARPLLAVIVITALGTVDNAVLSMKAGAYDFIT
                     KPFDVDTVVLAVRRAADYISVSKENTVLRNLHRNRMYFEDFIGDCPAVQSIKGLIPKL
                     AFADVPILVTGETGTGKNVLAKQIHFSLSGSESPLVYANCSSIPATLFESELFGHEKG
                     AFTGATALKKGRVESADGGTLLLDEISEIPYEVQAKLLDFLQERTFYRVGGVKPISVQ
                     IRIIALTNKNLQEEIQAGRFRKDLFYRLNVIHFHIPPLRERGDDVRLICEYLLSLLRK
                     KYNAPDIGLSEEALDVIASYPWPGNVRELKNVLERAFICADGGVIGAGDLILEEPERK
                     ADKRSLKGLTDEYERDVILRRLAFFRGNRTRTAEDLGVSLRNLQYKLEKYQIRG"
     gene            complement(5688..7496)
                     /locus_tag="P5313_11840"
     CDS             complement(5688..7496)
                     /locus_tag="P5313_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY81100.1"
                     /translation="MKLTTRLLILLFLSVLLPLLGLSFLLREEVTESILKEKEDKLFG
                     LARQLDRHLSGTFEDILREEGVLEAPRDRKIAVLNKRLWAITDFVASGNDGVGVGYYS
                     RDLDAVLTYGPSSEFQYTVGQSIFPGHRGYEVMATGKPLVQQGHLVRGNILNCMWPVV
                     REGRTIGYIWSNETVDQISGQLAPILRRVYGIVLLIFLLIYASVTVSTQTLLDKIYRI
                     KKGIEGLFEAPTRHIPPVRGELSVIVQTINELVDNMNLIKCYNKSILEGVSDGVLAVT
                     LDGRVSRANRAFYRLFPEHSDETLLGRGVREVFGPEIRGLVEAGMEDPEYRSAREIPS
                     GGRTLEAYGNRMIGEDGEPLGSVFVFRDVTPARRYEREVQEKERAVALGNMALGVVHE
                     VKNPLTSVKGFAQLLLRPEIPEDKRAEYVRLIDSELNRVNRLLNEMLVYGGKSRLELR
                     ECDILGILEDRVDNCEWCASPRPEIRARPGLSFVARVDEFKLAQVFDNLLKNASEAVS
                     AKGDGRIVAILREEAGWYALDLVDTGCGIRAEDLDRIGNPLFSTKRDGAGLGLAISRK
                     ILEAHGGKLSIESRLGFYTKVSLRLPRSGAAEAVPA"
     gene            7657..8688
                     /locus_tag="P5313_11845"
     CDS             7657..8688
                     /locus_tag="P5313_11845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY81101.1"
                     /translation="MHHPISFRARLSVALAAAAVSLTSCQPRKELPEGLYARLKTGRG
                     EILLRLEAEKAPLTVANFVGLAEGTLDAAAGKRYYDGLTFHRVVKDFMIQGGDPAGNG
                     SGGPGYRFPNETDTSLRHDGPGVLAMANSGPHTNGSQFYITHKAAPWLDGGYSVFGRV
                     VEGQSVVDSVQPGDRIESVEILRIGSNWEGYKADQALWNSLAEARTAKLREEREAARA
                     AETAQIDAKWPGLSEGPRGLRWKVLKEGSGPKLAHGDLARVDYQGMLTTGKTFDASRL
                     HGGPLELEVGTGQVILGWDLTLLDMKKGERRLVVIPPELGYGPAGAGGGVIPPDAFLV
                     FEMEVVGVR"
     gene            8696..9472
                     /locus_tag="P5313_11850"
     CDS             8696..9472
                     /locus_tag="P5313_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRY81102.1"
                     /translation="MAPGEVLRTADLARAAGTHVNTVRFYEAMGFLPPAERGPNGYRL
                     WKGEHRDQMVFARKALHGLWPGRRIRASALALVRKAAAEGPEAALPAARAHRDLVRQE
                     RARAEEAARFLESWARGRADAGDGRRAPRLGPREAARAAGATPGQVRNWERNRLIRTP
                     RETEGGRRTYGPGEIGRLRVIRTLLLAGYSVMAVQRMTRALDRGRDGELREVLDTPAP
                     GEEALTACDRWLSSLAEQEARAESLISVLEDRSTKKTTLQ"
     gene            9510..>10322
                     /locus_tag="P5313_11855"
     CDS             9510..>10322
                     /locus_tag="P5313_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRY81103.1"
                     /translation="MNDHPIQVRGARTHNLKNLSVDVPKDGIVVFTGPSGAGKSSLLF
                     DTIHTEAQRQLVETFSTFARRRLPKLTRPPVDEIRNLAVSIVVDQKPMGRTLRSTVGT
                     ATEIADYLRMLYSRFGSPFVGPSFYFSFNNPEGMCPECSGLGKRIRIDPGAFYDPARS
                     LREGALLHPDYKVGGYFWREFLSIDLFDPDLPLGRWKPADLERFLHTEPFPVEVRHGA
                     GTTKKNFEGVIRRLERYYANKAEDEAEEGEKDVYQSFLRYGTCPACGGRRVNP"
BASE COUNT         1685 a         3457 c         3462 g         1718 t
ORIGIN      
        1 catcctgtcc gtctcatggt tccaatgcgt tcaatcctca gcccaggacc cgcaggacgg
       61 tcacgcagtt ggtcaccgag gagcctccca cgttgagcac ggcacccacc ttgggggtgc
      121 ccttggaggg ggccgtcccg atggcgtggc cggtcaactg cttgtacagc agggcgtgca
      181 tggaggcgcc ggtggcgccc acggggtgcc ctttggcctt gaggccgccg gagaggttca
      241 cgcagacctt gtcgtcccgg ccgaagcgtc cggccaggta gtcgtcgccg gcctttccgt
      301 ccgcggacag gcccagggcc tcggtgcaga ggatctggtt gatggtgaag cagtcgtgga
      361 cctcggcgat gtcgatgtcc cggctcgcga tccccgcctc ggcgaaggcg cggcgggcgg
      421 cttccttgcc ggccatgagc tcgtgcatgt tcggacgcac cttgatgggc atccgctccg
      481 tcgtgtggcc gatgccggcg atctcgacgg cggggcggcc gcgcttccgg gcctcctcgg
      541 tccgcatgac caccagggcc gcggcgccgt cggtgaccag ggagcaatcg tgcagccgca
      601 ggggctccgc gatcatgggg ttcttgtcct ccgggaggcc caggatggcc tcggcggtga
      661 ccaggcccag cttgtccgag ggcccgccct tgccgaagtg agccaggggg ttgtccaggc
      721 cgtttcggta gcagagggcg gccacggcgg ccagcatctc cgccagcttc ttgtcggaaa
      781 tcttgtaccg gtcacggtag cccttggcgt actcggcgaa gagggccggg aagctcatac
      841 ccatcccgcc ctcggtgggc caatgggagg agcaggccag ggcgtgggtg acccccttgg
      901 tgtccaggag gttcatcttc tcgaccccga tcaccagggc cgcgtcgcag cgccccgcct
      961 ccacggcgta gaccgcgctg tacagggccg cggaggagga ggcgcaggcg tcctccaccc
     1021 ggtaggcggg cttaaagcgg agtcccttgg ggtccacctc cagggcgttg ggcgccaggt
     1081 gttcctggtt ggcgaagagg gagggcgagc aggaacccac ccagatcccc ccgatgcggt
     1141 ccgcctcgat cccggcgtcc gccaatgcgc ccctcccggc ctcctcgatg agctcgtaga
     1201 tgctcttgag gtccgtgacc tccttggttt ccttgttctt cttcacgaag gagccgaacg
     1261 ccgtgttgta ggcgccgact atgctggtct tgctcattcc atactccttg tcgatgtcgc
     1321 accgaaccgt tctcgaccgc gggccgcgcc tcgcttaccc gagatccact tcccggcagt
     1381 ccttgtccac cacgatgtcc gcgtcggtct tctccagtat gtagtccacc gtgtagggtt
     1441 tgaaatactc cttgagcacg aaggtcccgt tccggacctc gaagaacccg acgtcggtgc
     1501 agacgaagtc cacctgcccg gcggcggtca ggggcagctt gcacgcccgc aggagcttgg
     1561 aggccccgga cttgtcgaag tgctccgtgg cggccacgac gatcttagcg cccacggtca
     1621 ggtccatggc cccccccatg cccgggacca tcttgccggg tatcttgtaa ttcgccaggt
     1681 tgcccttctg gtccacttcc aggaccccca ggaccgtggc gtccacgtgc cccccccgga
     1741 tgatggcgaa gctcatggcg gagtcgaaga aggccccgcc gggctgtatg gtcacgaatc
     1801 ctcccccggc gttcaccagg gcggggtctt ccctgcccgg cgccggagcg ggccccaggc
     1861 ccagaaagcc gttctccgac tggaggacga tggtcacccc cgccggaacg tagttgccca
     1921 ccagggtcgg caggccgata cccaggttca ccacatcccc gtccttgaag atccgggcga
     1981 tgcgcttggc gatgacttcc ttgtttgccg tgtattccat gtccttaccc ctgcttcttc
     2041 gaccggacca ggtagtgcac gagcaccgag ggcgtcacca cgatctcggg gtccagctgt
     2101 ccgatctcga cgatctcgtc cgcctccacg atcacggtct cgcaagccat ggccatgagg
     2161 gggttgaagt tgcgggccgc cttgtcgtag acgaggttgc cggccttgtc ggccttctta
     2221 gccttgagca gggcgacgtt tcccggcagg gccttctcca gcaggaattt ccggccgtcc
     2281 acgtccagga cctgcttgcc ttcctggacc tcggtgccca cgcccgtggg ggtgaggacg
     2341 cccgccaggc cagcgccggc cgctcggatc ctctcggcca gggtaccctg gggcactagg
     2401 accacctcgg tctccccctc gttcatctgc cgctgggtct cctggttcgt gccgatgtgg
     2461 gagaccacga tcttccggaa ctgcttcttg gccaccagct tggccacgtg ggtgcaggcg
     2521 cccgtcttga aattcaggaa cgccgtgtcg ttgcagacca gggtcagatt cttcgttccc
     2581 tgttgttcca gggcgtcgat cagggccgga ggggacccgc agcccaggaa gccgccgacc
     2641 atgacggtgt cgccttcctt gatcagctcc acggccttct cggccgcgat aatcggtttc
     2701 gctaccatgt ctatcgctcc taacgttcaa aacaaccttc gcgcctcgga cggactcccg
     2761 gcggcccggc ctgcgaagtg tacgtatgta tcgagccgta tgccgggagg aaaccgggtc
     2821 cggcggggcg cacccgccgg acccggcgtc aagcgacggg aatcagagga agagcagtcc
     2881 cagggaggtg atgatgccca cccagagcat gatcacggtg gtgtagccca tgatgtcctt
     2941 gatgcccagc ttggccaccg ccaggagggg cagggcccag aagggctgga tcatgttggt
     3001 ccaggcgtct ccccaggcga tggccgaggc ggttcgggcg tagctgactc ccagggcctg
     3061 ggcggcgggc atcatgatcg gaccctggac ggcccactgg ccgccgccgg agggtacgaa
     3121 catgttcaca aggcccgcgg accagtaggt gaagagctgg aaggtcttgg gcgtcgaaat
     3181 ggacacgaac cagttggaga aggtggcggc caggccggag ctgaccatca tgcccatgat
     3241 ccccgcgtag aaggggaact ggaggacgat gccgccgcag ttgcgcacgg acttgtccac
     3301 ggccttcacg tagttgatcg gggtcttgtg catgaggagg cccaggacga ggaagatgaa
     3361 gttgaccacg tccagggaga gggagccgcc cttcgcgagg tagcggaccg catacacgag
     3421 ccccaccaca ccgacgatga tcgccagtac gatggaattt tcgatcttct cggcgggggt
     3481 catccccttg aaggacttcg tcgcgacttc ttccttctcg tcctccgcga agatcgaggg
     3541 gtccacctcg acgatctcct ccttcttctt gggcatcata aggacgttga tgatcggcac
     3601 cgtgaccagg agcacgagga cgatgatcag ggtctggggg gagaagatgg tctcaccagt
     3661 ggggatgatg gtccccgggg cgaccttctc catgaccggg ttcttggccg cgatggtcag
     3721 ctggatggag gccgacagac ccgagtgcca aagcacgaag cccgaatagg agctggccac
     3781 gagaagggga tagtggatgc ccttggtgac cttggcgatc tccttggcca ggatcgcgct
     3841 gatgatgagc ccgaagcccc agttgagcca gcagccgatg cccatgacga aggcgacgat
     3901 ggcgatggcc ttggcggggg tgttggccgc ggacgcgatg gtcttgagga ccctgtgcac
     3961 cggcttggac agggcaaggg cggagcccgt gaccaggacc aggaccatct gcatggagaa
     4021 gcccaggagg ctccacatgc cgccgcccca ataggacgtc atctcgatga acgacttgcc
     4081 ctggatgacc agccccagca cgaacaccag gatggtcagg atgatcgcga agatgaacgc
     4141 gtcggggatc cacttcttcg cgatcttggt aaagaaattg cctagagccc ggatcatacg
     4201 gcctcctctt gagatgtgtt gagaacacta aagcaagttc cgtgccaggc tcgaaaacgg
     4261 ccgtccggcg gcgttcgggg actaggcggc cttcccgggg aatggggaaa cgcgcaagat
     4321 ccgtcgtgga ccgcgcaaga tcttgcgcgc ctaaccccgg atctggtact tctcgagctt
     4381 gtactggagg ttccgcaggg agacgcccag gtcctcggcg gtgcgggtcc ggttaccccg
     4441 gaagaaggcc aggcgccgga ggatgacgtc cctctcgtac tcgtccgtga ggcccttgag
     4501 ggaccgcttg tccgccttgc gctcgggctc ctccaggatc aggtcccccg ccccgatgac
     4561 ccccccgtcc gcgcagatga aggccctctc caggacgttc ttgagctccc ggacgttccc
     4621 gggccagggg taggaggcga tcacgtccag ggcctcctcg gacaggccga tgtccggtgc
     4681 gttgtacttc ttccgcagga gggacagcag gtactcgcag atcagccgga catcgtcccc
     4741 ccgctcccgc aggggcggga tgtggaaatg gatgacgttc aagcggtaga acaggtcctt
     4801 ccggaaccgc cccgcctgga tctcctcctg gaggttcttg ttggtcaggg cgatgatgcg
     4861 gatctggacc gagatgggct tgacgccccc cacgcggtag aaggttctct cctgcaggaa
     4921 gtccaggagc ttggcctgaa cctcgtaggg gatctcggag atctcgtcca ggaggagggt
     4981 acccccgtcc gcgctctcca cccggccctt cttcaaagcc gtcgcccccg tgaaggcgcc
     5041 cttctcgtgg ccgaagagct cggactcgaa gagggtggcc gggatgctgg agcagttggc
     5101 gtagaccagg ggggattcgg agccggacag cgagaagtgg atctgcttgg ccaggacgtt
     5161 cttgcccgtg ccggtctccc cggtcacgag gatgggcacg tccgcgaagg ccagcttggg
     5221 gatgaggccc ttgatggact ggaccgcggg acagtccccg atgaagtcct cgaagtacat
     5281 ccggttcctg tggaggttcc gaaggaccgt gttctccttg ctcacgctga tgtagtccgc
     5341 ggcccgcctc accgccagta ccacggtgtc cacgtcgaag ggcttggtga tgaagtcgta
     5401 ggccccggcc ttcatggaca ggaccgcgtt gtccacggtc cccagggcgg tgatcacgat
     5461 gaccgccagg agggggcggg cggccttcag atccgggatg atgtccgttc cgttgcagtc
     5521 cgggagcttc aggtccagga tgcagaggtc gggcttggcg gtccgggcgg catccagggc
     5581 cgccttgccg gagtccgcct ccaccacctc gaagccctcc aggaccagga ggtcccggag
     5641 gaagacacgg acgttccgct cgtcgtcgca gaccaggata gtgtacttca agccggcacc
     5701 gcctcggccg cgccggaccg cgggagccgc aggctgacct tggtgtagaa cccgagccgg
     5761 gactcgatgg aaagcttccc gccgtgggct tccaggatct tccggctgat ggccaggccc
     5821 aggccggcgc cgtcccgctt ggtggagaag agggggttgc cgatccggtc caggtcctcg
     5881 gcccggatcc cgcagcccgt gtccaccagg tccagggcgt accaccccgc ctcctcgcgc
     5941 aggatcgcca cgatgcgccc gtcccccttc gcggacaccg cctcggacgc gttcttgagg
     6001 agattgtcga agacctgggc cagcttgaac tcgtccaccc gggcgacgaa ggacaggccc
     6061 ggccgggcgc ggatctcggg ccgcggggag gcgcaccact cgcagttgtc cacccggtcc
     6121 tccaggattc ccaggatatc acactcgcgg agctccagcc ggctcttccc gccgtagacc
     6181 agcatctcgt tcaaaaggcg gttgacccgg ttcagctccg agtcgatcag ccggacgtac
     6241 tcggcgcgct tgtcctccgg aatttccggc ctcaggagca gctgggcgaa acccttcacg
     6301 gaggtgaggg gattcttgac ctcgtgcacg acccccaggg ccatgtttcc cagcgccacc
     6361 gccctctcct tctcctggac ctcccgttcg tagcgccggg ccggggtcac gtcccggaac
     6421 acgaagaccg agcccagggg ctctccgtcc tccccgatca tccggttgcc gtaggcctcc
     6481 agggtccggc cgcccgaggg gatctcccgg gcggaacggt attccggatc ttccataccg
     6541 gcttcgacca ggccgcggat ctccggcccg aagacctccc ggaccccgcg gcccaggaga
     6601 gtctcgtccg aatgctccgg gaagaggcga tagaaggccc ggttggcccg cgacacgcgg
     6661 ccgtccaggg tgaccgccag gaccccgtcg gaaaccccct ccaggatgct cttgttgtag
     6721 cacttgatga ggttcatgtt gtccacgagc tcgttgatgg tctggacgat gacggacagc
     6781 tccccccgca ccggggggat gtgccgggtg ggggcctcga agagcccctc gatccccttc
     6841 ttgatgcggt agatcttgtc caggagggtc tgggtggaga ccgtcacgct ggcgtagatg
     6901 agcaggaaga tgagaagcac gatcccgtag acccggcgca ggatgggggc cagctgaccc
     6961 gagatctggt ccaccgtctc gttggaccag atgtagccga tcgtccggcc ctcccggacc
     7021 acgggccaca tgcagttcag gatgttgccc cgtacgaggt ggccctgctg taccaggggc
     7081 ttgcccgtgg ccatgacctc gtagcccctg tgccccggaa agatggactg tcccacggtg
     7141 tactggaact ccgagctcgg gccgtaggtc aggaccgcgt cgaggtcccg gctgtagtag
     7201 cccaccccca ccccgtcgtt tccggaggcc acgaagtccg tgatcgccca cagacgcttg
     7261 ttgagcacgg cgatcttccg gtcccgggga gcctccagga cgccctcctc ccggagtatg
     7321 tcctcgaagg tccccgaaag gtgccgatcc agctgccggg ccaggccgaa gagcttgtcc
     7381 tccttctcct tgaggatgct ctcggtgacc tcctcccgga gcaggaagga caggcccagc
     7441 aggggcaaca gcacgctcag gaagaggagg atgagaagcc gggtggtcaa tttcatggga
     7501 agggaacctc gacttcgagc ctaccacggg ggccgtccgg ggacaaggcg gtccgacgcg
     7561 cggcccggac ccggggccgg ccgtcacaag cttcgtgaca ccgggaagac cttgccaaag
     7621 gacgccgtgt acgccaagat tcgccccgga ggaaccatgc accatccgat ctcgttccgg
     7681 gcccggcttt ccgtggccct cgcggccgcg gccgtctccc tgacttcctg ccagccccgc
     7741 aaggaactgc ccgagggcct gtacgcccgc ctcaagaccg gccgggggga gatcctcctg
     7801 cgcctggagg ccgagaaggc ccccctgacg gtcgccaact tcgtgggcct ggcggaaggc
     7861 accctggacg ccgcggcggg caagcgctac tacgacggac tcaccttcca ccgggtggtg
     7921 aaggatttca tgatccaggg aggggacccc gcggggaacg gttccggggg tccgggctac
     7981 cgcttcccga acgagacgga cacgtccctg cgtcacgacg gccccggggt cctggccatg
     8041 gccaactcgg gcccccatac caacggttcc cagttctaca tcacccacaa ggccgcgccc
     8101 tggctggacg gggggtattc ggtcttcggc agggtcgtgg agggccagtc cgtggtggac
     8161 tccgtccagc cgggggaccg catcgaatcc gtggagatcc tccgtatcgg ctcgaactgg
     8221 gaggggtaca aggccgatca ggccctatgg aactccctgg ccgaggcacg gacggccaag
     8281 ctgcgggagg agcgcgaggc ggcccgggcc gccgaaacgg cccaaatcga cgccaagtgg
     8341 ccgggcctgt ccgaggggcc ccggggtctc cgctggaagg tcctcaagga gggttccggg
     8401 cccaagctgg cccacgggga cctggccagg gtcgactacc aggggatgct gacgaccggc
     8461 aagaccttcg acgcctcccg cctccacggc ggccccctgg aactggaggt gggaaccggc
     8521 caggtgatcc tgggctggga cctcaccctc ctggacatga agaagggcga gcgccgcctg
     8581 gtggtcatcc cgccggagct gggctacggc ccggcggggg ccggaggcgg ggtcatcccc
     8641 ccggatgcct tcctggtctt cgagatggaa gtggtcgggg taaggtaggg tccccgtggc
     8701 gccgggcgag gtcctccgca ccgccgacct ggcccgggcg gcgggaaccc acgtcaacac
     8761 ggtccggttc tacgaggcga tgggcttttt gccccccgcg gaacggggtc ccaacgggta
     8821 ccgactctgg aagggggagc accgggacca gatggtcttc gcccggaagg ccctgcacgg
     8881 cctctggccg ggtcggcgga tccgggcctc cgccctggcc ctggtccgca aggccgccgc
     8941 ggagggtccc gaggcggccc tgcccgcggc ccgggcccac cgggacctcg tgcgccagga
     9001 gcgcgcccgg gcggaggagg cggcgaggtt tctggaatcc tgggcccggg gacgggccga
     9061 tgcgggcgac ggccgacggg ctccccgcct gggtccccgg gaggccgccc gggcggccgg
     9121 ggccacgccg gggcaggtcc gcaactggga acgtaaccgc ctgatccgga ccccccggga
     9181 aaccgagggg ggccggcgga cctacggccc cggggagatc ggcaggctcc gggtgatccg
     9241 caccctcctc ctggcgggct actcggtcat ggcggtccag cgcatgaccc gggcattgga
     9301 ccggggccgg gacggggagt tgcgggaggt cctggacacc cccgccccgg gagaggaagc
     9361 tctcacggcc tgcgaccgat ggctcagctc cctggcggag caggaagccc gggcagaatc
     9421 cctgatctcg gtcctggagg atcggtcgac gaaaaagaca accctacagt agcgcaccac
     9481 cccagccgac cccggctatc cttcccccca tgaacgacca ccctatccag gtgcgcggag
     9541 cgcgcaccca caacctcaag aacctgtccg tggacgtgcc caaggacggg atcgtcgtgt
     9601 tcacgggtcc cagcggggcg ggcaagagct ccctgctgtt cgacaccatc cacaccgagg
     9661 cccagcgcca gctggtggag accttctcca ccttcgcccg gcgccgcctg cccaagctga
     9721 cccgcccccc ggtggacgag atccgcaacc tggcggtctc catcgtggtg gaccagaagc
     9781 ccatggggcg gaccctccgg agcaccgtcg ggacggccac ggagatcgcc gactacctcc
     9841 ggatgctcta ttcccggttc ggctcgccct tcgtcggccc ctccttctac ttctccttca
     9901 acaacccgga ggggatgtgc ccggagtgct cgggcctggg caagcggatc cggatcgatc
     9961 ccggggcctt ctacgacccg gcccggagcc tccgggaggg ggccctcctc cacccggact
    10021 acaaggtcgg gggctacttc tggcgggagt tcctctcgat cgacctcttc gatcccgacc
    10081 tgcccctcgg ccggtggaag cccgcggacc tggaacgctt cctccacacc gagccgttcc
    10141 ccgtggaggt ccggcacggg gcgggcacca cgaagaagaa cttcgagggg gtcatccggc
    10201 ggctggagag gtactacgcc aacaaggcgg aggacgaggc ggaggagggg gaaaaggacg
    10261 tctaccagag cttcctccgc tacgggacct gccccgcctg cgggggccgc cgggtgaatc
    10321 cc
//
LOCUS       DAOTEI010000127        10268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4584_length_10268_cov_4.764907, whole genome shotgun sequence.
ACCESSION   DAOTEI010000127 DAOTEI010000000
VERSION     DAOTEI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4584_length_10268_cov_4.764907"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..749)
                     /locus_tag="P5313_11860"
     CDS             complement(264..749)
                     /locus_tag="P5313_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81104.1"
                     /translation="MRAFPIPPALREYARVFKDAGRQAWLVGGALRDMILGKKVNDWD
                     VATDAPAQEVARMFPRVIPTGIQHGTVTVLWKGLTIETTTFRVDGDYGDGRRPDSVTF
                     TSDIREDLRRRDFTMNSLAYNLETGELLDDLQGLQAEELPVGMGHDLMVAHAGEMKLM
                     G"
     gene            843..1469
                     /locus_tag="P5313_11865"
     CDS             843..1469
                     /locus_tag="P5313_11865"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRY81105.1"
                     /translation="MERALLVIGGEAPPRAALEPLFGSFGAVCAADSGLEVVHDWGLS
                     PTLIVGDMDSLTRPELLSAYPGARIVCADRAKDETDTEMGLRLLAVAGNREIVLAGGG
                     GGRLDHLLAVRALFEREIRPREWWTARERVVLADRPFEADLPPGTVVSVFPLARGARG
                     MRSSGLRWPLDGLSWGPGDFGVSNEAEGGTMRVEPGEGDLLVVLPLGD"
     gene            complement(1466..1951)
                     /locus_tag="P5313_11870"
     CDS             complement(1466..1951)
                     /locus_tag="P5313_11870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRY81106.1"
                     /translation="MEYFFNFDKLGYLKGGRPEGCILCLVKDGSDQVERLVVHETERF
                     LVSLNLYPYNPGHLLVFPRRHVTDVRELDGAEKAELTALTDLCLDALERAFSPGGFNI
                     GYNMGLVAGASIDHLHLHIIPRFPREIGIVELIAGKRVLVQDPLETQDRLRKVFAEIA
                     P"
     gene            complement(1956..2798)
                     /locus_tag="P5313_11875"
     CDS             complement(1956..2798)
                     /locus_tag="P5313_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81107.1"
                     /translation="MKKFITWMLVLIVLAGAVFAAGWIQFRVPAGQYGVYVTKTRGWH
                     SEVLVPGRFAWSWEALIPSNFRLFRFALEPRTETVTLSGTLPSSEVYAAFAVGSPDFS
                     YEASYGIVASVRPEALPKAAESRKIETQEALDAWLSEGIRTAGESLRGLLLARASDPD
                     WIAAVLRGEPGTSEDLAARIGAAVPDLEIRSVSVRSIRVPDLDLYEAVKAKYLGFLAS
                     ADSALSRTLEREAQARAQTELRLESLERYGQLITKYPKLIDFLAVENRTDAALLEALG
                     KRVE"
     gene            2936..3280
                     /locus_tag="P5313_11880"
     CDS             2936..3280
                     /locus_tag="P5313_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81108.1"
                     /translation="MKDSVAARSVWKSAFKRAALYFLGLDLLAFFYYLLGNYQGFLSD
                     TQIMLLRFVSGLSAAAFLSGLAGLARGVFAALRRREALPLAAAVGWLACMAAAVVLAL
                     LSQGVRAFAAGI"
     gene            3386..4888
                     /gene="gltX"
                     /locus_tag="P5313_11885"
     CDS             3386..4888
                     /gene="gltX"
                     /locus_tag="P5313_11885"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRY81109.1"
                     /translation="MSVRVRYAPSPTGLQHIGGVRTALFNYLFARANGGKFILRLEDT
                     DRTRSSEEYVKNLYDTFDWLGIRWDEGPDVGGPKGPYVQSERFGLYRQYAEKLVAAGK
                     AYYCFCGEDRLSRMRSDAEPEEGEGAASYGYDRACRNIESAEARRRAEAGEAHVIRLK
                     IPLEGTTKFRDLLLGEIEWKNADVSPDPVLLKSDGFPTYHLANVVDDHLMEITHVMRA
                     QEWIPSAPMHMIMYEAFGWTPPSLCHLPMVLGSDGHKLSKRHGATAVNEFRTKGYVKE
                     ALINYVAMLGASYEDGRDLYSLEDLARLFRIDRINKAPAVFDYVKLEWFNGQYIRAKE
                     DGELTEMLLPFAVDAGLFGAPGTRPSGGQDSLFRSAVPLVKERLSYLSEAPAKLAYLF
                     SEPPLAPVEEFIPKKLDAPRAAELLAIAAALLPDCDLSDIPASEEKFRTKAAEAGCKL
                     GDLMMPLRVAVTGSRVSPPLFESIRLLGLPAVLARAAKARAALAAAGSAP"
     gene            4885..5352
                     /locus_tag="P5313_11890"
     CDS             4885..5352
                     /locus_tag="P5313_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY81110.1"
                     /translation="MIDQRRPAAYERIYRQLEPLIREKSPNLVAGMATVAALLHGKLR
                     HHFWTGFYFVAGPEELHVGPYQGSVACQVLKGRGVCLHAVKTGAPVVVPDVEAFPGHI
                     ACDSRSKSEIVLPLRKDGRVVAVLDIDSDKPAQWDSDDVEPLARIVSLLEPLA"
     gene            complement(5411..5884)
                     /locus_tag="P5313_11895"
     CDS             complement(5411..5884)
                     /locus_tag="P5313_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HRY81111.1"
                     /translation="MNPTKELLDRISAEEAALALDRFDEEDAWRLGLLLTEEARRRKA
                     PVAVDVRRPGQILFHAALPGATPDNDEWVRRKSACAFRFSKSSLAVGVGLALAGKTLE
                     EKSFVSSFEYSAHGGAVPIRVRNVGIVACATVSGLPQEEDHALVVECLRRLAGGA"
     gene            complement(5929..6360)
                     /locus_tag="P5313_11900"
     CDS             complement(5929..6360)
                     /locus_tag="P5313_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRY81112.1"
                     /translation="MSVNQVSIFLENSKGRLAEVTRILAKEGIDLRALSLADTSDFGV
                     LRIIVADPDRCLGVLKENGFVARTTEVVAVEVEDRPGGMSRVLDVLDEAGLNVEYMYA
                     FVAKAREHAVVVCKIDERERAREVLKAKGIRTISAAELRGL"
     gene            complement(6387..7685)
                     /locus_tag="P5313_11905"
     CDS             complement(6387..7685)
                     /locus_tag="P5313_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRY81113.1"
                     /translation="MIWNPDYESLPPDGLKRLQDERLGTLVDMLYRRVAFFRTKMDAS
                     GIKPGDIRGIGDLARLPFTTKDELRSGYPFELLAVDLREIVEIHTSSGTTGKPVVDAY
                     TAGDLDTWAEAMARTLSAGGATRHDIVQNAYGYGLFTGGLGVHYGARRIGATVLPISG
                     GNTKRQVELLRDFGTTVLTCTPSYALYLAEALREEGMKPRDMGLRAGFFGAEPWSDTM
                     RSEIQASLGLAAYDIYGLTEIIGPGVAFECERQDGLHVNEDLFYPEIVDPETGRVLPD
                     GQKGELVITTLTKQGTPILRYRTRDITYLDRTPCPCGRTTVRMHRLLGRTDDMLIVRG
                     VNVFPSQIEEVILAIEGVEPHYQIIVDRRNQLDSLEVQVEMTEGLFSDEIRDLERTEK
                     LIESRLYGTLNIHSRVKLVEPKTIARSEGKAKRILDKRQI"
     gene            7835..8635
                     /locus_tag="P5313_11910"
     CDS             7835..8635
                     /locus_tag="P5313_11910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRY81114.1"
                     /translation="MPTARTSSSSSSITGYFVLSYLLFWVLLGVTGFLIFLKAPGPLV
                     AAMKNVCAWAPTFALAILFRRLNPGTSWKDFLRAQVAEKPRAGLFLAVLAAQAGILVL
                     ARAVHSALGGQPFAAWTLPAPAALAAAFVVNLTSGPLGEELGWRGYALPRLRRTRSTT
                     ASGLLLGLVWGFWHLPLWLMSGFSGPDLLRYVLAFLAAIVSVSVLIAHALERNRNILL
                     AVWIHFWFNFLVQLAGIDILALMTYIAVGYGFAAVLVVALAERRPAAP"
     gene            8882..9154
                     /locus_tag="P5313_11915"
     CDS             8882..9154
                     /locus_tag="P5313_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY81115.1"
                     /translation="MGKKLYVGNLSYNTGENSLRELFQNYGTVASAKIITDRDSGNSK
                     GFGFVEMGSDSEAQAAIAGTNGTDLDGRQIKVNEAMDKPRRDDNRW"
     gene            9284..9493
                     /locus_tag="P5313_11920"
     CDS             9284..9493
                     /locus_tag="P5313_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 17 family protein"
                     /protein_id="HRY81116.1"
                     /translation="MNHSGWIFMAIGLFSVVCAAFDFEWFMNHRKARFFVRILGRTGA
                     RVFYIVLGAAILVLGGLVLFGALHT"
     gene            complement(9522..10259)
                     /gene="ribA"
                     /locus_tag="P5313_11925"
     CDS             complement(9522..10259)
                     /gene="ribA"
                     /locus_tag="P5313_11925"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRY81117.1"
                     /translation="METLNSPEEILASCDNTMSRAELEAKLKGKPDYYAEEYFCAGKG
                     AYCRDEELCVQLVARARLPTRFGEFEIAGFYDAKHDKEHTAVLKGTVEGAVDLPLRIH
                     SQCHTGDVFGSLRCDCRDQLEAALRYVEAEGRGAVVYMKQEGRGIGLLNKIKAYRLQE
                     LGLDTVEANRYLGFPDDARDYAVTAAIIRLLGIKSIRLMTNNPDKIEGMRKEGIEVTG
                     RIPVVIEPNPHDGSYLETKRDRMGHLY"
BASE COUNT         1558 a         3470 c         3398 g         1842 t
ORIGIN      
        1 ccatcctgcg cgatttcatt ggccaggtct actggacctc cttcgccgcc ggacgcacag
       61 ccatcatcaa ggagcaggcg ctggccttta tcgacggcgc cgtacaaaac ggccatatcg
      121 ccgcaccctg gtcggccgag cttcgtgacc gcatcgcaag aggcctgatg acctgcctgg
      181 ccgattttga tctggcgcaa aaaggccggg ccttgtcgcg caaaatcgtc agttttcgcc
      241 cctttccatc caccatcttt tttctagccc atgagcttca tttcgccggc atgagcgacc
      301 atcagatcat ggcccatccc gactggcagc tcttcggctt gcagcccctg gaggtcgtcc
      361 aggagctctc ccgtctccag attgtaggcc agggagttca tcgtgaagtc ccggcgccgg
      421 aggtcctccc ggatgtcgga ggtgaaggtc accgaatccg gtcgccttcc gtccccgtag
      481 tccccgtcca cccggaaggt ggtggtctcg atggtgagac ccttccagag gaccgtgacg
      541 gtgccgtgct ggatccccgt gggtatgacc cgggggaaca tccgggccac ctcctgggcc
      601 ggggcgtcgg tagccacgtc ccagtcgttc accttcttgc ccaggatcat gtcccggagg
      661 gccccgccca ccagccacgc ctggcgtccg gcatccttga agacgcgggc gtattcgcgg
      721 agggccgggg ggatcgggaa ggcgcgcatc ggggctcctt gaccaccgtt gtaccgaatt
      781 ccagcccccg ggacaaggga ccctcggttg acaggccgga cccgggcgga cacactcgtc
      841 ccatggaacg agccttgctg gtgatcggag gggaggcgcc gccccgggcc gccctggaac
      901 ccctgttcgg gtccttcggg gccgtgtgcg cggcggactc gggcctggag gtcgtccacg
      961 actggggcct ctcgccgacc ctcatcgtcg gggacatgga ttccctcacc cgtcccgagc
     1021 tcctgtccgc ctatcccggg gcgcggatcg tctgtgcgga ccgggccaag gacgagacgg
     1081 acacggagat gggcctccgg ctcctggcgg tggcggggaa ccgcgagatc gtcctggccg
     1141 gcgggggcgg gggacgcctg gaccacctcc tggctgtccg ggccctcttc gagcgggaga
     1201 tccggcctcg ggaatggtgg accgcccggg agcgggtcgt cctggcggac cgccccttcg
     1261 aggcggacct tccgcccggc accgtcgtct cggtctttcc ccttgcccgg ggggcccggg
     1321 gcatgcggag ttccgggctc cgttggcccc tggacgggct ttcctggggt cccggggatt
     1381 tcggggtgag caacgaggcc gagggcggga cgatgcgggt ggagccgggg gagggggacc
     1441 tcctggtcgt ccttcccctg ggcgattaag gggcgatttc cgcgaagacc ttccgcaacc
     1501 ggtcctgggt ttccaaggga tcctggacca ggacgcgctt gccggcgatc agttccacga
     1561 tgccgatctc ccgggggaag cggggtatta tgtgcaggtg gaggtggtcg atggaggccc
     1621 cggccacgag ccccatgttg tagccgatgt tgaatcctcc gggggaaaag gcccgctcca
     1681 gggcgtccag gcagaggtcc gtcagggcgg tcagttcggc cttttcggcc ccgtccagtt
     1741 cccggacgtc ggtgacgtga cggcggggaa agaccaggag gtgccccgga ttgtacggat
     1801 agaggttcag ggataccagg aacctctccg tctcgtggac caccaggcgt tcgacctggt
     1861 ccgatccgtc cttgaccagg cagaggatgc atccctcggg ccttccgccc ttcaggtatc
     1921 ccagcttgtc gaagttgaag aagtactcca tcccgctact cgacccgctt ccccagggct
     1981 tccaggaggg ccgcgtcggt gcggttctcc acggccagga agtcgatcag cttggggtac
     2041 ttcgtgatga gttggccgta acgctccagg gactccaggc ggagctccgt ctgggcccgg
     2101 gcctgggctt cccgctccag ggtgcggctc aaggcgctgt ccgcggatgc aaggaatccc
     2161 aggtacttgg ccttgacggc ctcgtagagg tcgaggtccg gaacccggat cgagcggacc
     2221 gagacggagc ggatctccag gtccgggacc gcggctccga ttcgggcggc caggtcctcc
     2281 gaagtacccg gttccccccg gaggaccgcg gcgatccagt cggggtccga ggcccgggcc
     2341 aaaaggaggc cccggaggct ctcccccgcg gtccggatgc cctcggaaag ccaggcgtcc
     2401 agggcttcct gggtttcgat cttccgggac tccgcggcct tgggcagggc ttccggccgg
     2461 accgaggcga cgatcccgta ggacgcttcg tacgagaaat ccgggctgcc caccgcgaag
     2521 gccgcgtaga cttccgagga ggggagggtg ccggagaggg tcacggtctc ggtcctcggt
     2581 tccagggcga accggaagag ccggaaattc gaggggatca gggcttccca ggaccaggcg
     2641 aagcggccgg gcaccaggac ctcggaatgc cagccccggg tcttggtcac gtagaccccg
     2701 tactggccgg cgggcacccg gaactgtatc catccggccg cgaacacggc tcccgcgaga
     2761 acgatcaaca cgagcatcca ggttatgaac tttttcatcc gggttcctcg agggtccggt
     2821 tgacccggtt tcgagtctat ccgattccgg ggagcatttc cataccgggt tttccggtct
     2881 gcggatccct tgcgcgatcc gcccgggaca tgtacctttc tgtcgggagg atccgttgaa
     2941 ggattccgtt gccgcgcgtt ccgtctggaa gtccgcgttc aagcgggccg ccctgtattt
     3001 cctcggcctg gacctcctgg cctttttcta ttacctcctg ggcaactatc aagggttcct
     3061 gtcggatacc cagatcatgc tgttacggtt cgtgtccggt ctttccgccg cggccttcct
     3121 gtccgggctc gcggggctgg cgcggggcgt gttcgcggcc ctcaggcggc gggaggccct
     3181 gccccttgcg gcggccgtcg ggtggctcgc gtgcatggcc gccgcggtgg tcctggccct
     3241 gctttcccag ggggtccggg cgttcgccgc gggcatctga ccggaagccc ggattcttga
     3301 cccccccttc ccggcccgac tataatcccc gtcacgacga gtccaaatcc ggcgcgccgt
     3361 ccgcgccgcg tccctaggag tcaacatgag cgtccgagtc cgctacgccc cgtcgccgac
     3421 gggactccag catatcggcg gggtgcgcac cgccctgttc aactacctct tcgcccgggc
     3481 caacgggggg aagttcatcc tgcgcctgga ggacacggac cgcacccggt cctccgagga
     3541 gtacgtgaag aacctctacg acaccttcga ctggctgggg atccgctggg acgagggacc
     3601 cgacgtgggc ggcccgaagg ggccctacgt gcagtccgag cgcttcggtc tgtaccgcca
     3661 gtacgctgaa aagctcgtgg ccgcgggcaa ggcctactac tgcttctgcg gggaggaccg
     3721 gctctcccgg atgcgctccg acgcggaacc cgaggagggg gagggcgcgg ccagctacgg
     3781 ctacgaccgg gcctgccgga acatcgagag cgcggaggcc cggcgccggg ccgaggcggg
     3841 ggaggcccat gtcatccgcc tcaagatccc cctggagggg accacgaagt tccgggacct
     3901 cctcctcggc gagatcgagt ggaagaacgc cgacgtgtcc ccggaccccg tgctcctcaa
     3961 gagcgacgga ttcccgacct accacctggc gaacgtggtg gacgaccacc tcatggagat
     4021 cacccacgtg atgcgggccc aggagtggat accctcggcg ccgatgcaca tgatcatgta
     4081 cgaggccttc ggctggacac ctccctcgct ctgccacctc cccatggtcc tgggctcgga
     4141 cgggcacaag ctgtccaagc gccacggggc cacggcggtc aacgagttcc ggacgaaagg
     4201 ctacgtgaag gaagccctga tcaactacgt ggccatgctg ggggccagct acgaggacgg
     4261 ccgggacctc tactcactgg aggatctggc tcggctcttc cggatcgacc ggatcaacaa
     4321 ggctcccgcg gtcttcgact acgtcaagct cgagtggttc aacggccagt acatccgtgc
     4381 caaggaggac ggtgagctta cggaaatgct cctgcccttc gccgtggacg cggggctctt
     4441 cggagccccg ggaacccggc cctccggcgg ccaggacagc ctcttcaggt ccgcggtgcc
     4501 cctggtcaag gagcgccttt cgtacctgag cgaggcgccc gccaagctgg cctacctctt
     4561 ctccgagccg cccctggccc cggtcgagga gttcatcccc aagaagctgg acgctccccg
     4621 ggccgcggag ctcctggcca tcgccgccgc cctgcttccg gactgcgacc tgtccgacat
     4681 ccccgccagc gaggagaagt tccggaccaa ggccgccgag gccggctgca agctggggga
     4741 cctgatgatg cccctgcggg tggccgtcac gggcagccgg gtgagccccc ccctcttcga
     4801 gtccatccgc ttattgggcc tgcccgcggt actggcccgg gcggccaagg cccgggcggc
     4861 cctggccgcg gccgggagcg ccccgtgatc gatcagcgcc gccccgccgc ctacgagcgg
     4921 atctaccggc agttggagcc cctgatccgg gagaagtccc ccaacttggt tgcgggcatg
     4981 gccacggtcg cggccctcct gcacggcaaa ctgaggcacc acttctggac agggttctac
     5041 ttcgtcgcgg gtcccgagga gctccacgtg ggaccctacc agggctccgt ggcgtgccag
     5101 gtgctcaagg ggcggggggt ctgcctgcac gccgtgaaga ccggggcccc cgtggtagtc
     5161 ccggacgtgg aagccttccc gggccacatc gcctgcgact cccggtccaa gagcgagatc
     5221 gtcctgcccc tcaggaagga cggccgggtg gtggcggtcc tggacatcga ctccgacaag
     5281 cccgcacagt gggactccga cgacgtggag cccctggcga ggatcgtgag cctcctggag
     5341 cccctggcct agcggaccgt ccgtgccgcc gcccacggat gccgccggca tggagcgatg
     5401 gacgggacgc tcaagcccct cccgccagcc tccggaggca ctccaccacc agggcgtggt
     5461 cctcctcctg ggggaggccg gacacggtcg cgcaggcgac gatccccacg ttccggaccc
     5521 ggatcggaac ggcgcccccg tgggcgctgt actcgaagga actgacgaag gacttctcct
     5581 cgagggtctt tcccgccagg gccaggccga cccccacggc cagggaggac ttggaaaagc
     5641 ggaaggcgca ggcggacttg cggcggaccc actcgtcgtt gtccggggtg gctcccggaa
     5701 gggccgcgtg gaagaggatc tgcccgggcc gccgcacgtc cacggccacg ggggccttcc
     5761 gcctccgggc ctcttccgtc aggaggagac ccaagcgcca ggcgtcctcc tcgtcgaaac
     5821 ggtccagggc gagggcggct tcctccgcgg agatccggtc caggagttct tttgtagggt
     5881 tcatggacgg agtatacccc gtgcggggaa ggaccgtcac ggttccggct acagaccccg
     5941 tagttccgcc gccgagatcg tccgtatccc tttcgccttc aggacctccc gggcccgctc
     6001 gcgctcgtcg atcttgcaga cgaccacggc atgttcccgg gccttggcca cgaaggcgta
     6061 catgtattcc acgttgagtc ccgcctcgtc caggacatcc aggacccggg acatcccgcc
     6121 cggccggtcc tccacctcca cggccacgac ctccgtggtc cgggccacga aaccgttctc
     6181 cttcaggacc cccaggcagc gatcgggatc cgccacgatg atgcggagca ccccgaagtc
     6241 cgaggtgtcc gccagggaca gggcccgcag gtcgatccct tccttggcga ggatccgggt
     6301 cacctccgcc agccggcctt tggagttctc caggaatatg gaaacttggt tcacgctcat
     6361 atcgcctccc cgggtccgag cccggctcag atctgccgct tgtccaggat ccgcttggcc
     6421 ttgccttcgc tgcgcgcgat agtcttgggt tccaccagct tgacccggga atggatgttc
     6481 aaggtcccgt agagccggct ttcgatgagc ttttccgtgc gctccaggtc gcggatctcg
     6541 tcggagaaga ggccctcggt catctccacc tggacctcca ggctgtccag ctggttcctg
     6601 cggtccacga tgatctggta gtggggctcg acgccctcga tcgccaggat gacctcctcg
     6661 atctgggagg ggaagacgtt caccccccgg acgatgagca tgtcgtccgt tcgcccgagg
     6721 aggcggtgca tccggaccgt ggtccgcccg caggggcagg gcgtgcggtc caggtaggtg
     6781 atgtcccggg tccggtatcg gaggatgggc gtgccctgct tggtcagggt ggtgatgacg
     6841 agttctccct tctggccgtc cgggaggact cgccccgtct cgggatccac gatctccggg
     6901 tagaagaggt cctcgttgac gtggagcccg tcctggcgct cgcattcgaa ggccaccccg
     6961 ggaccgatga tctccgtgag cccgtagatg tcgtaggccg cgaggcccag gctcgcctgg
     7021 atctccgagc gcatggtgtc gctccagggt tccgccccga aaaacccggc tcggagcccc
     7081 atgtcccggg gcttcatccc ttcttcccgg agggcttccg ccaggtacag ggcgtaggaa
     7141 ggggtgcagg tcagtaccgt ggtgccgaag tcccgcagga gctccacctg gcgcttggtg
     7201 ttcccgccgg agatgggcag gaccgtggcc ccgatcctgc gggcgccgta gtgcaccccc
     7261 aggccgccgg tgaacaggcc gtagccgtag gcgttctgga cgatgtcgtg ccgggtggcc
     7321 ccgcccgcgg agagggtgcg ggccatggcc tccgcccagg tgtccaggtc tcccgcggtg
     7381 taggcgtcca ccacgggctt gccggtggtc cccgaggagg tgtggatctc cacgatctcc
     7441 cggagatcca ccgccaggag ctcgaagggg taccccgagc gcaactcgtc cttggtggtg
     7501 aagggcagac gggccaggtc gccgatgccc cggatatccc cgggcttgat ccccgaggcg
     7561 tccatcttgg tacggaagaa cgcgaccctc cggtacagca tgtccaccag ggttcccagg
     7621 cgttcgtcct gcaggcgctt gagcccgtcc gggggaaggg actcgtagtc gggattccag
     7681 atcaaggcgg attctccttc cggtcttcgc ggcggcagtg atcggcggca gtctggcgaa
     7741 ccggattgat tctgtcaaga gaaacatggc aaaaaatccg gaggcctggt gagttcgctc
     7801 cgggttccgt acaatacggg gcaaggagaa ccgcatgccc acagcacgaa cctcctcctc
     7861 ctcctcctcc attaccgggt atttcgtcct ctcgtacctc ctgttctggg ttttgctggg
     7921 ggtgaccggg ttcctgattt tcctgaaggc tcccggaccc ctggtcgcgg cgatgaagaa
     7981 cgtgtgcgca tgggctccca ccttcgccct ggcgatcctc ttccggaggt tgaatccggg
     8041 aacgagctgg aaggacttcc tccgggccca ggtggccgag aagccccggg cgggactctt
     8101 cctggccgtc ctggccgccc aggcgggcat cctggtcctg gcccgggccg tccactccgc
     8161 cctgggggga cagcccttcg ccgcctggac gctcccggcc ccggccgcac tggccgccgc
     8221 cttcgtcgtg aacctcacct cgggccccct cggggaggaa ctgggatggc ggggttacgc
     8281 ccttccccgg cttcgccgga caaggtcaac gaccgcgtcc ggactgctcc tgggcctggt
     8341 ctgggggttt tggcacctgc ctctgtggct gatgtccgga ttctccggcc cggacctcct
     8401 tcggtacgtc ctggccttcc tggcggccat cgtctccgtg tcggtcctca tcgcccacgc
     8461 cctggaacga aaccggaaca tcctgctggc cgtctggatc catttctggt tcaatttcct
     8521 ggtccagctc gcggggatcg atatcctggc ccttatgaca tacatcgccg taggatacgg
     8581 gttcgcggct gtcctggtcg tagccctcgc ggaacgccgt cccgccgcgc cctgaacagg
     8641 ggtccgcggg aggcgatccg agatgaggca gccggggcgc agccgacctt ccggccgacg
     8701 ggatccccgg atcgagcgaa ttccgtcggc ccgagtccgg ccctacccat ccttccggaa
     8761 atagggtata ctgccgtcag cttgatcgcc gatccgcgag gatttccgat tcggtcatcc
     8821 gcgtcttctg cggaacatgt tgaggatacc cgctctacgt cggcgggtgg aggaaaagat
     8881 tttgggcaag aaactgtatg taggaaacct gagctacaat accggagaga acagcctccg
     8941 ggagctgttc cagaattacg gcaccgtagc gtccgccaag atcatcacgg accgggattc
     9001 cggcaattcc aagggcttcg gcttcgtgga gatgggttcg gacagcgaag cccaggccgc
     9061 catcgccggg accaacggca ccgacctcga cggccggcag atcaaggtca acgaggccat
     9121 ggacaagccc cgccgggacg acaaccgctg gtagatccgg ccgcgttccg cgcctgaaag
     9181 ccgccccctt ccggggcggt ttttcttttt ggacttcccg tccgcatctc cggaaggtag
     9241 aatcgccggg atattccgaa tcgtgcgaaa cgggggagct aacatgaatc attccggctg
     9301 gatcttcatg gccatcgggc ttttctctgt ggtctgcgcg gccttcgatt tcgagtggtt
     9361 catgaaccac aggaaggccc ggttcttcgt gaggatcctg ggccgaacgg gcgcccgggt
     9421 gttctatatc gtgctggggg cggcgatcct ggtcctggga ggactggtcc tgttcggcgc
     9481 cctgcatacc taggcacccc ggccggcggg ccggggcccg gtcaatacag atggcccatg
     9541 cggtcccgct tggtctccag gtagcttccg tcgtggggat tgggctcgat gaccacggga
     9601 atccggccgg tcacctcgat gccttccttc cgcatcccct cgatcttgtc cgggttgttg
     9661 gtcatgagac ggatcgactt gatcccgagc agacgaatga tcgcggcggt caccgcgtag
     9721 tcccgggcgt cgtccgggaa tcccaggtag cggttggcct ccaccgtatc caggccgagt
     9781 tcctggaggc ggtaagcctt gatcttgttc aataggccta tgccccggcc ctcctgcttc
     9841 atgtacacca cggcgccccg tccctcggcc tcgacgtacc tcagggccgc ctccagctgg
     9901 tcccggcagt cgcagcggag gctgccgaac acgtccccgg tatggcactg ggagtggatg
     9961 cgcaggggca ggtccaccgc tccttccacg gtccccttga gcacggcggt gtgctccttg
    10021 tcgtgcttgg cgtcgtagaa ccctgcgatc tcgaactctc cgaaccgggt cgggagccgg
    10081 gcccgcgcga cgagctggac gcagagctcc tcgtcccggc agtaggcgcc ttttccggcg
    10141 cagaagtact cctccgcgta gtaatcgggc ttccccttga gcttggcttc cagctcggcc
    10201 cggctcatcg tgttgtcgca ggatgcaagg atctcttcgg gcgagttcaa ggtttccatc
    10261 cgcgtctc
//
LOCUS       DAOTEI010000128        10266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4587_length_10266_cov_6.791891, whole genome shotgun sequence.
ACCESSION   DAOTEI010000128 DAOTEI010000000
VERSION     DAOTEI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4587_length_10266_cov_6.791891"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="P5313_11930"
     CDS             <1..358
                     /locus_tag="P5313_11930"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY81118.1"
                     /translation="PRTFPLIVILSLFGLSVVLGIQGLLRSLRDRNPLPPTLPRGKTA
                     GKLAVLTAAGAGYTLILEPVGYILATPVLIAVAMILFGERRPIRIAILSLTASAALYF
                     LFRGVFRVPLPRSILW"
     gene            373..1863
                     /locus_tag="P5313_11935"
     CDS             373..1863
                     /locus_tag="P5313_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY81119.1"
                     /translation="MPGLLEGFVYAFSHLHFLAIGVVGGILVGVIPGLSGSTGIILML
                     PFLLYVEPSAALIMMSGLFCGSMYGGSISAILISTPGTPSAAATVLDGYPLAKKGQAG
                     KALWVATIASTAGGMVSALCLIFLAPQLARIALKFGPAEYFALMIFGLTIMASVSASS
                     ITKGLISGFFGILLSLVGIDPTLGTERFTFGVVRLLTGFSMMPVLIGVFALSEVLLQL
                     ELLSDGKLARIPHTDTLGSIVPTKKERKGLWKVILGSSILGTIIGIIPATGGAIASFM
                     AYNEARRFSKEPETFGTGNLAGIAAPEAANNGTTGGAMVPLITLGIPGDVVTAVMLGA
                     LLLIGVQPGPLLFSQTPHIVNNLFAGFLVAQFLILGFGLLSIRVSPLILKAPLAVLFP
                     LILCLCLLGAFSLGNSMYDVGLAIIFGAIGYLMKKHGFSAASLILGLILGPIAERELS
                     RALILSHGNWLTLVKSPIAVGFYVLALASVGYSLAGSGKAGKANKA"
     gene            complement(1868..2587)
                     /locus_tag="P5313_11940"
     CDS             complement(1868..2587)
                     /locus_tag="P5313_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRY81120.1"
                     /translation="MYTAIKSEGQRSQEVAARIKDLILSEKLKRGEKIPNELNLCGLF
                     SVSRPTVREAIKSLVSAGILEIRRGKGTYVSMLPGLAEDPLGLDFVSGPDLRISLLEA
                     RLIIEPGVARLATRNADVQDVERIARSIRDMEEIVHLEKVDVNPELAFHRSIAEATKN
                     PVIMRLVPVIMDSIVKTYRDAPRTSEDHRHALQEHTAIFKAIAARDAEGAYGAMHDHL
                     ERSYRRTMAKRSVRGASESPG"
     gene            complement(2707..3480)
                     /locus_tag="P5313_11945"
     CDS             complement(2707..3480)
                     /locus_tag="P5313_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY81121.1"
                     /translation="MRESTDWAEEYGKLLGRLRVREEDCDETVLAHHLPFLSTLDAVD
                     GSSVALFDLRTKSYRFLTSSFKFLGDYPRDEALALGPNYFFRLMLPADLPFVLETVTR
                     ALRFLLAVPAEERRDYRLSFEFRIRAAGGGLVRLVQQVVILEADARGNPWLVLIANDR
                     APRGAGEETLLRRLENRRAGTWHLFPPGDRGEGPGTLSPRETEILGLVASGLASREIA
                     DRLYISVATVNNHRQRILEKLSTKSSAEAVRYAAALGLI"
     gene            3589..4047
                     /locus_tag="P5313_11950"
     CDS             3589..4047
                     /locus_tag="P5313_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81122.1"
                     /translation="MTESERLKTLQLMYAGVLVDAVRQYGSFGILANVTEKKRIEQEL
                     AARDQVARLGIHTPEDLFTILPEIFGCAAWNPDDSGPVFRVETRSCLMCALAKRLGAP
                     APCSIYCVNPSRALAGALPVPHDLTVLGTLWDGGSCVFELEPRGAGESAR"
     gene            4282..6402
                     /locus_tag="P5313_11955"
     CDS             4282..6402
                     /locus_tag="P5313_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRY81123.1"
                     /translation="MPNSFPDRPLVPVIGVDEDKCVNCHMCIAVCPVKFCIDGSGEKV
                     SIDETLCIGCGSCVRACSQKARYIIDDADHLLEDLERGERIVAVAAPALVSSFPGRHR
                     KFLGWLRSLGVDACFDVSFGAELTVRSYLDHIRTNDPRLVIAQPCPAIVSYVEIYRPG
                     LIPYLAPADSPMLHTIKMIREYHPEYSRHKVVVVSPCAAKKREFEETGIGDYNVTIRS
                     FHRILEERGVDLDHLPESDFDNPPAERAVLFSTPGGLLRTAMRDAPGIESRTRKIEGP
                     ELVYPYLDTLQSSLESGIAPLLVDCLNCEFGCNAGPGTLNHGKSPDAFERSVEDLAEE
                     RRRRYGGRAASDRAVRRRLAKVVSGRWRPGLYGRNYVDRSPDLRIREPDDARLAEIYA
                     ALLKETEKDHLNCAACGYKTCRGMAVAIHNGLNKKENCHLFRQRLIEREQTIVHEANA
                     RLYREIRQATGQVDAIRTALNRLQEDAASQFSAIEESAAAVEQMVATLGNASSIASSK
                     RGQMDHLAEEASAGGRDMDRTVESIRKATGGMNAIDGMIASIRDVSDRTNLLAMNAAI
                     QAARAGNAGRGFAVVAGEIRGLAETTGRNVGSITGDLSEIIAQIRDSDGMVSRTGDRI
                     RSMSRDVVTMAGELSALVDSLAELSSGGSQVTSGIEELRNLSMGVKEVYGQMASGVGS
                     ILEMINTIAAISEETQRTVDTAGG"
     gene            6457..7311
                     /locus_tag="P5313_11960"
     CDS             6457..7311
                     /locus_tag="P5313_11960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRY81124.1"
                     /translation="MRRIVPEEGMKPHGTVPGSRELRFFLLATFAWTWFFWLGLAGLA
                     RGGLVSAGTAPFILLHMAGGFGPTILALVCAARRAGDRPLAGFLHTWLPGRLSPAGFA
                     ALGAGLVLAVLKPLVLRGLNPGTPLLVENWPAMLALFPVMILGGGLEEPGWRGIVFED
                     SERLRSGDLGALAGMAVLWTAWHLPLWLIPGTYQNTSMDLVSFGLGVGGSTLVFYALR
                     MSGASVGWCVLAHAFYNTALGTFVPNTNLASDRLVSAAVLAAALAFFLVFRGRLRART
                     VRSGDAPA"
     gene            7321..8217
                     /locus_tag="P5313_11965"
     CDS             7321..8217
                     /locus_tag="P5313_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY81125.1"
                     /translation="MAELVIEDVGGGTRIVRGPTNLGILEARDGTAVLVDSGNDEDAG
                     RKLLRRLEADGLRLSLIVNTHSNADHCGGNAFLQARTGCRIAATREEAAFVENPLLEP
                     SLLWGGYPPASLRNKFLMAKPSRVTDVLAPSGPIPGMELEAVPLPGHFVGMIGVRTPG
                     GVLFCADALASAEILAKYPVFFLYDVAAHYRTLDFLASCPAELFVPSHAAPVREIGPL
                     VEVNRRAVDRVNEVVLDLARPEPSWEDLLAGLVTRLGIELNHSQYALVHASIRAHLAY
                     LADTGALATRFESGKLRVGCRP"
     gene            8280..8483
                     /locus_tag="P5313_11970"
     CDS             8280..8483
                     /locus_tag="P5313_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRY81126.1"
                     /translation="MTQNMGTIDRVIRIAAAIGIAVLYAVGLISGTTAIVLGFFAVVF
                     LATSFLGFCPLYVPFKISTRKKG"
     gene            8535..9392
                     /locus_tag="P5313_11975"
     CDS             8535..9392
                     /locus_tag="P5313_11975"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY81127.1"
                     /translation="MDIPLQAIHARTSIRSYDEVALSSGDREVLVRTFQDCLPGPFGG
                     RPRFALTESGALGSGGRIGTYGVIRGAPAYIAGAVKPEPFAFVDFGYCLEGVVLAATD
                     RGLGTCWLGGFFDRRAASRILGLGPGEVVPAASPVGRPAEGRRTLMERVIRGSAGSSR
                     RKPFETLFFDGGFDRPLSPKEAGPWAEVLECVRIGPSASNKQPWRILRDASAETPAFH
                     LFLDEDRLYSRAIPGILLQDLDAGIAMRHFEAAARALGLPGGWSRLEPSPPVPKTSFK
                     YVSTWTEPH"
     gene            9436..10212
                     /locus_tag="P5313_11980"
     CDS             9436..10212
                     /locus_tag="P5313_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81128.1"
                     /translation="MIDSIGKAVRGKLDSFLYGLGFFVSVLRETGRFFRRRQVGYKVL
                     VMQILFTGFEALRINAVMSLGIGAAIIVIGTSLLPQFGQSQLLYTILIIVITRELGPL
                     LTAFVVIARSGTAIATELGTMVVSHEIEAYLSVGVNPISYLVVPRFLGMVASMLILTI
                     YFNIFGLLGSFLVVQVVRPVGFQEYFRNLLTTLTVSDILSGLGKSVIFGAITSVVATY
                     QGFSVTRASTEIPVAGIRAVGQSFMLCILADVVITLMQYV"
BASE COUNT         1615 a         3463 c         3263 g         1925 t
ORIGIN      
        1 cccgcgtacc tttcccctga tcgtgatcct gtcactcttt ggtctttccg tcgttctggg
       61 aattcagggg ctgctccgat ccctgcgcga caggaatccc ctaccgccca cactgccccg
      121 gggaaagacc gccggcaagc tcgcggtcct gacggcggcg ggtgcgggct ataccctcat
      181 cctggaaccc gtcggctaca tcctggctac gccggtcctg atcgccgtgg ccatgatcct
      241 cttcggcgag cgccgcccga tccgaatcgc gatcctttcc ctgaccgctt cggcggccct
      301 ctatttcctg ttccgggggg tgttccgggt cccccttccc cgctcgattc tctggtgata
      361 ggagatcccg ccgtgccagg attacttgaa ggtttcgtct acgccttttc ccacctgcat
      421 ttcctggcca tcggagtggt cgggggtatc ctagtggggg tcattccggg actgtccggt
      481 tccacgggga tcatactgat gctgcccttc ctcctgtacg tagaaccctc cgcggccctc
      541 atcatgatga gcggcctgtt ctgcggatcc atgtacggag gttcgatttc cgccatcctg
      601 atctcgactc ccggaacccc ctcggcggcg gccacggtac tggacggcta ccccctggcg
      661 aagaaaggcc aggcgggcaa ggctctttgg gtcgcgacga tagcgtccac tgcgggagga
      721 atggtatccg ccctctgcct catattcctg gcgcctcaac tggcccggat tgccctgaag
      781 ttcgggcccg ccgagtactt cgcactgatg atcttcgggc tcaccatcat ggccagcgta
      841 tccgcctcgt cgatcactaa gggcctgata tcgggttttt tcggaattct cctctccctg
      901 gtgggaatcg atccgaccct gggcaccgag cgcttcacct tcggcgtagt ccgcctgctt
      961 acgggattct ccatgatgcc cgtccttatc ggcgtcttcg ccctttcgga ggttctgctt
     1021 caactggaac tgctcagcga cggtaagctc gcccggattc cccataccga cacgctcggt
     1081 tccatcgttc ccacgaaaaa agaacggaag ggtttgtgga aggtgattct cggatcctcc
     1141 atcctgggaa ccatcatcgg catcatcccc gccacgggag gggccatcgc ctccttcatg
     1201 gcctacaacg aggcccgccg tttctcgaag gagccggaaa ccttcggcac gggaaacctg
     1261 gcgggcatcg ccgctcccga ggcggcgaac aacggaacca ccggaggggc catggtcccc
     1321 ctgatcaccc tgggaattcc gggggacgtg gtcaccgcgg tcatgctcgg tgccctgctc
     1381 ctgatcggtg tccaaccggg accgctgttg ttcagccaga ctccgcacat cgtgaacaac
     1441 ctgttcgcgg gtttcctagt cgcccagttc ctgatcctcg ggttcggact attgagcatc
     1501 cgcgtgtcgc ccctcatcct gaaggcgccg cttgccgtgc tcttccccct catcctctgt
     1561 ctctgcctgc tgggggcatt ttccctgggc aacagcatgt acgacgtggg cctggctata
     1621 atcttcgggg caatcggcta cctcatgaag aagcacgggt tctccgcggc ttccctgatc
     1681 ctgggcctca tcctcggccc catcgccgag cgggagttga gccgtgccct catactgtcc
     1741 cacgggaatt ggctcacctt ggtcaaatct ccgatcgccg tcgggttcta cgtcctggcc
     1801 ctggcgtccg tggggtattc cctggccggt tcgggcaaag ccggcaaggc gaataaagcc
     1861 tgagggatca gcccgggctt tccgaagctc cccggaccga gcgcttggcc atggtccgtc
     1921 ggtagctccg ttctagatgg tcgtgcatgg cgccgtaggc gccctcggcg tcccgggcgg
     1981 cgatggcctt gaagatggcc gtgtgctcct gcagggcgtg gcggtggtcc tcgctcgtgc
     2041 ggggggcgtc ccggtaggtc ttcacgatgg agtccatgat gaccggaacg agccgcatga
     2101 tcacggggtt tttggtcgcc tcggcgatac tccgatggaa cgcaagttcc gggttcacgt
     2161 cgaccttttc caggtggacg atctcctcca tgtcgcggat gcttcgggct atgcgttcca
     2221 cgtcctggac gtcggcgttc cgggtggcca gccgcgcgac cccgggttca atgatcagcc
     2281 tcgcctctag gagggaaatt cgtaggtccg ggcccgatac aaaatcgagg cccaggggat
     2341 cctcggccag gccgggaagc atggaaacgt aggtgccctt tccccggcgg atctcgagga
     2401 ttccggcgga taccagggac tttatggcct cccgcacggt gggccgggac acggagaaaa
     2461 gtccgcagag attcagctcg ttggggatct tctccccacg cttcaatttc tccgaaagga
     2521 tgaggtcctt gattcgggcg gcgacttcct gggagcgctg cccttcggat ttgatagcag
     2581 tatacacggt acacctgtta gtttggatcg ttgactgaca tcatacaatg ataccatggc
     2641 gcgttaaaac gcacaactcg aggcggcggc ggtccggccc tggcacgcgg ttgcgaatcc
     2701 gtggactcag atcaggccga gggccgcggc atagcgtacg gcctcggcgc tggacttagt
     2761 gctgagcttc tccaggatcc gttggcgatg gttgttcacc gtcgccacgc tgatgtacag
     2821 gcggtcggcg atctcgcggc tggccaagcc cgaggcgacg agccccagga tctcggtctc
     2881 ccggggagac agggttcccg gcccctcgcc gcggtccccc ggcgggaaga ggtgccaggt
     2941 cccggccctc cggttctcca ggcgtcggag cagtgtctcc tcccccgccc cccggggcgc
     3001 ccggtcgttc gcgatcagga cgagccaggg attcccccgc gcgtccgcct ccaggatgac
     3061 aacctgctgg acgagccgga ccagcccccc tccggcggcg cggatccgga actcgaagga
     3121 aagacggtag tccctccgtt cctctgcagg aaccgccagg aggaagcgca gggcccgggt
     3181 cacggtctcc agcacgaagg gcaggtccgc cgggagcatg agccggaaaa agtagtttgg
     3241 tcctagggcc agggcctcgt cccggggata atcgcccagg aacttgaagg acgaggtcag
     3301 gaagcggtag ctcttggtcc gcaggtcgaa gagggcgacg gaactcccgt ccacggcgtc
     3361 cagagtcgag aggaagggca ggtgatgggc caggacggtc tcgtcgcagt cctcctcccg
     3421 gacccgtagc cgcccgagaa gcttcccgta ttcctccgcc cagtcggtcg attcccgcac
     3481 cccggtcctc cgatggaata ccgtaccgaa tgatgatttc tcatcatacc gcgccccggt
     3541 aaacccgggt agagtgcggg tatacgatcg agcaggcggg aggaagggat gacggaatcg
     3601 gaacggctga agacacttca actgatgtac gccggtgttt tggtggacgc ggtccggcag
     3661 tacggctcct tcgggatcct ggcgaacgtc acggagaaga agcggatcga gcaggagctt
     3721 gcggcccggg accaggtcgc ccggctcggg atacatacac ccgaagacct cttcacgatc
     3781 ctccccgaga tcttcggctg cgccgcctgg aacccggacg actcgggccc ggtcttccgg
     3841 gtggagaccc ggtcctgcct gatgtgcgcc ctggccaagc ggctcggcgc cccggcgccc
     3901 tgctccatct actgcgtaaa cccctcccgg gctctggccg gggccctccc ggtcccgcac
     3961 gatctcaccg tgctcggtac cctctgggac ggcggctcct gcgtcttcga gctggagccc
     4021 cggggggcgg gggaatcggc ccgctaggga ggtcccgggg acgacgggtc ccgcctccgc
     4081 gccggaacgg gcgggacggg acgccgcggc gtctccccac gcgattctcg tacggcggac
     4141 cgattctccc cggggaatgc tttatcgata atttcacatt gcgaaatgaa attctaaaac
     4201 acgatggact atactctagg tattccttcc ccaggatccg ccgtgcgtac cgctgggaaa
     4261 tccgaccccg agaggaatac tatgccaaat tccttcccag atcgtcccct ggttcccgtc
     4321 atcggggtgg acgaggacaa atgcgtgaat tgccatatgt gcatcgccgt ttgtcccgtc
     4381 aaattctgca tcgacggctc gggagagaag gtttccatcg acgagaccct ctgcatcggc
     4441 tgcgggagct gtgtgcgcgc ctgctcgcag aaggcccgtt acatcatcga cgacgccgac
     4501 catctccttg aggatctgga acggggcgaa cgtatcgtcg cggtagccgc cccggccctc
     4561 gtgtcgagct tcccggggcg gcaccggaaa ttcctcggat ggctccgcag cctcggcgtc
     4621 gatgcctgct tcgacgtgag cttcggtgcc gaactgaccg tccggtccta tctggaccac
     4681 atccgcacga acgatccccg actcgtgatc gcccagccct gtccggcgat cgtgagctac
     4741 gtcgagatct accgccccgg actgatcccc tacctggccc ccgcggacag ccccatgctc
     4801 cacaccatca agatgatccg ggagtaccat cctgaatatt cccgccacaa ggtagtcgtg
     4861 gtatccccct gcgccgcgaa gaaacgcgag ttcgaggaga cggggatcgg ggactacaac
     4921 gtcacgatcc gttccttcca ccggatcctc gaggagcggg gcgtcgacct ggatcatctg
     4981 cccgaatctg acttcgacaa tcctcctgcg gaacgggccg tcctcttctc cacccccggc
     5041 ggcctcctcc gaaccgccat gcgggacgcg ccggggattg aatcccggac ccgcaagatc
     5101 gagggccccg aactcgtata cccctacctc gacaccctcc agtcctccct ggagtcgggc
     5161 atcgcacccc tcctggtgga ttgcctcaac tgcgagttcg gctgcaacgc gggacccggc
     5221 acgctgaacc acggcaagtc gccggacgcc ttcgagcggt ccgtggagga tctcgccgag
     5281 gaacggcgcc gacgctacgg ggggcgggcc gcgtccgacc gggccgtccg tcgccgcctg
     5341 gccaaggtcg tctccggccg gtggaggccc ggactgtacg ggcggaacta cgtggaccgc
     5401 tcccccgacc tccgtatccg ggagcccgac gacgcacggc tggccgagat ctacgcggcc
     5461 ctgctcaagg agacggaaaa ggaccatctc aactgcgccg cctgcggcta caagacgtgc
     5521 cgaggtatgg ccgtggccat ccacaacggc ctgaacaaga aggagaactg ccacctcttc
     5581 cgccaacgcc tgatcgagcg agagcagacg atcgtccacg aggccaacgc ccggctgtac
     5641 cgggagatcc gccaagccac cggccaggtg gacgcgatac ggaccgccct gaaccggctg
     5701 caggaggacg cggcctccca gttctcggcc atcgaggaat ccgcggcggc cgtggagcag
     5761 atggtggcca ccctggggaa cgcgagctcc atcgcgtcca gcaagcgcgg ccagatggac
     5821 cacttggcgg aggaggcctc ggcgggcggg cgagacatgg accggaccgt ggaatcgatc
     5881 cggaaggcga cgggcggcat gaacgcgatc gacggcatga tcgcctcgat ccgggacgtg
     5941 tcggaccgga cgaacctgct ggccatgaac gcggccatcc aggccgcccg ggcggggaac
     6001 gccgggaggg gcttcgccgt cgtggccggt gaaatccggg gcctggcgga gacgaccggc
     6061 cggaacgtcg ggagcatcac cggggacctc tcggagatca tcgcccaaat ccgggactcc
     6121 gacggcatgg tctcccgaac gggggaccgg atccgctcca tgtcccggga cgtcgtgacc
     6181 atggcggggg agctgtccgc cctggtcgac tccctcgccg agctctcctc gggggggtcc
     6241 caggttacct cgggaatcga ggaactgcgg aacctctcca tgggcgtcaa ggaggtctac
     6301 ggccagatgg cctccggagt cggatcaatc ctggagatga tcaacaccat cgcggccatc
     6361 tccgaggaaa cccagagaac cgtggacacc gcgggggggt gacggatcgg ccattggcgg
     6421 aaacccgacg ctccggtatg atcgattccg ggcgggatgc gacgcatcgt cccggaggaa
     6481 ggcatgaaac cgcacggcac agttccgggc tcccgggagc tccggttttt cctgctcgcg
     6541 accttcgcct ggacctggtt tttctggctc ggcctggcgg gtctggcccg ggggggcctg
     6601 gtatccgcgg gcacggcgcc cttcatcctc ctccatatgg ccggcggctt cggtcccacg
     6661 atcctggccc ttgtatgcgc cgcccgccgc gccggggaca ggcccctggc gggcttcctg
     6721 catacctggc tgcccggaag gctcagcccc gcgggcttcg cggccctggg ggccgggttg
     6781 gtcctggcgg tcctcaagcc cctcgtcctt cggggcttga atccgggtac accgctcctg
     6841 gtcgagaact ggccggccat gctcgccctc ttcccggtca tgatcctggg cggcggcctg
     6901 gaggagcccg gctggcgggg aatcgtgttc gaggattcgg agcggctgag gtccggagac
     6961 ctcggcgccc tggccggcat ggccgtcctc tggaccgcct ggcacctgcc gctttggctg
     7021 atcccgggaa cttatcagaa cacgagcatg gacttggtct ccttcggcct gggcgtggga
     7081 ggttccactc tggtctttta cgccctccgg atgagcggcg cctccgtggg ctggtgcgtc
     7141 ctggcccacg ccttctacaa caccgcgttg ggtactttcg taccgaacac gaacctcgct
     7201 tcggatcgcc tggtttccgc ggcggttctc gcggcggccc tcgccttctt cctggttttc
     7261 cgaggccggc tccgggcgcg tacggttcgc tccggcgacg ctcccgccta aggaggagtc
     7321 atggcggaac ttgtcatcga ggatgtcggc ggcgggacgc ggatcgtccg gggacccacg
     7381 aacctgggga tcctcgaagc ccgggatgga acggcggtcc tggtcgattc gggcaacgac
     7441 gaggacgcgg gccggaagct cctgcgccgc ctggaggcgg acggcctgcg tttgtccttg
     7501 atcgtgaaca cccactccaa cgcggaccac tgcgggggaa acgcctttct ccaggcccgc
     7561 acggggtgcc ggatagcggc cacccgggag gaggccgcct tcgtggaaaa ccccctcctg
     7621 gagcccagcc tcctgtgggg cgggtatccc ccggcctcgc tccggaacaa gttccttatg
     7681 gccaagccat cccgggtcac cgatgtcctg gcgccgtcgg gccccatccc gggtatggaa
     7741 ctggaggccg tgcccctgcc gggccacttc gtggggatga tcggggtgcg tacccccggg
     7801 ggagtcctct tctgcgcgga cgccctggcc tccgcggaga tcctggccaa gtacccggtc
     7861 ttcttcctgt acgacgtggc ggcccactac cggaccctgg acttcctggc gtcctgcccg
     7921 gcggagctct tcgtcccgag ccacgccgcc ccggtccggg agatcggtcc cctggtggag
     7981 gtcaaccgcc gggccgtgga ccgggtgaac gaggtcgtcc tggacctggc ccggcccgag
     8041 cctagctggg aggatctcct ggccgggttg gtgacccgcc tggggatcga gctcaaccac
     8101 tcccagtacg cccttgtcca cgcctccatc cgggcccacc tggcttacct cgcggatacc
     8161 ggcgccctgg cgacccgttt cgagtccggg aagctccggg tcggttgccg gccgtgagga
     8221 atttgacttt tccctatata ttataaacct tgaatatagg atttttcttg gaggctatca
     8281 tgacccagaa catgggaacc atcgatcgag tcatccgcat cgccgccgcg atcgggatcg
     8341 ccgtcctgta tgccgtaggt ctgatttccg ggaccaccgc catcgtcctg gggtttttcg
     8401 cggtcgtctt cctggcgacc agcttcctgg gcttctgccc gctctacgtc cccttcaaga
     8461 tttcgactcg gaagaagggc tgaagcaact cgaaggcact tcaccggatt cgtgtatgtc
     8521 tgtatcttgg ggatatggac atacccctcc aagccatcca tgcccgcacc tccatccgtt
     8581 cctatgacga ggtggccctc tcgtccggcg accgtgaggt ccttgtccgg accttccagg
     8641 actgtttacc cggtcctttc ggaggccggc cgaggttcgc cctgaccgag tcaggggccc
     8701 tcggctccgg cgggaggatc ggcacctacg gggtgatccg gggcgcgccg gcgtacatcg
     8761 cgggcgcggt caaaccggag cccttcgcct tcgtggactt cggatactgc ctggaaggcg
     8821 tcgtcctggc cgcgacggac cggggcctgg gcacctgctg gctcgggggc ttcttcgacc
     8881 gaagggccgc ctcccggatc ctgggcctgg gtcccgggga ggtggtgccc gcggcgagcc
     8941 ccgtgggccg ccccgcggag ggacgaagaa ccctgatgga gcgggtcatc cggggctccg
     9001 cgggatcctc ccgtcgcaaa cccttcgaga ccctcttctt cgacgggggc ttcgaccggc
     9061 cgctctcgcc caaggaggcg ggaccctggg cggaggtcct ggagtgcgtg cggatcggac
     9121 cctccgcctc gaacaagcag ccttggagga tcctccggga cgcctcggcg gaaactcccg
     9181 ccttccacct cttcctggac gaggaccggt tgtacagccg ggccataccg ggaatcctcc
     9241 tccaggacct ggacgcgggg atcgcgatgc ggcacttcga ggccgccgcc cgtgccctgg
     9301 gcctgccggg cgggtggtcg cggcttgagc cctccccgcc cgtcccgaag acctccttca
     9361 agtacgtttc cacatggaca gaaccgcatt gattcgaagg ccgtcctgat ccatactgta
     9421 tccaggacac cccggatgat cgactcgatc ggcaaagcgg ttcgcggaaa actcgattcc
     9481 ttcctctacg gcctgggttt cttcgtgagc gtgctccggg agaccgggcg cttcttccgc
     9541 cgccgccagg tgggctacaa ggtcctggtc atgcagatcc tgttcacggg cttcgaagcc
     9601 ctccgcatca acgcggtcat gtccctggga atcggggcgg cgatcatcgt gatcggcacc
     9661 tccctcctgc cccagttcgg ccaaagccag cttttgtaca ccatcctgat catcgtgatc
     9721 acccgggagc tcgggcccct gctcacggcc ttcgtggtga tcgcccggtc cgggaccgcc
     9781 atcgccacgg agctggggac catggtggtg agccacgaga tcgaggccta cttgtcggtg
     9841 ggggtgaatc cgatctccta cctcgtggtg ccccggttcc tggggatggt ggcgtccatg
     9901 ctcatcctga ccatctactt caacatcttc gggctcctgg gttccttcct ggtggtccag
     9961 gtggtgcgcc ccgtgggctt ccaggagtac ttccgcaacc tcctcaccac gctcaccgtc
    10021 tcggacatcc tctcggggct gggcaagagc gtcatcttcg gggcgatcac ctccgtggtc
    10081 gccacctacc agggcttctc ggtcacccgg gcgtccacgg agatccccgt ggcgggcatc
    10141 cgcgcggtgg gccagagctt catgctctgc atcctggcgg acgtggtgat caccctgatg
    10201 cagtacgtgt gaggcggcga tgggcgagga agcggcgaat cccgtcatag agctgcgctc
    10261 cgtcac
//
LOCUS       DAOTEI010000129        10229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4608_length_10229_cov_4.663947, whole genome shotgun sequence.
ACCESSION   DAOTEI010000129 DAOTEI010000000
VERSION     DAOTEI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4608_length_10229_cov_4.663947"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="P5313_11985"
     CDS             complement(<1..497)
                     /locus_tag="P5313_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRY81129.1"
                     /translation="MTGLLFDVRKYSIHDGPGIRTAFFLKGCPLACPWCHNPEGLAGK
                     SELIFRPDRCLGTSACGACRTVCPRGADPSACVACGLCAEACPAEARQIVGFPLSVDE
                     VLLRARQDETFYDESGGGVTFTGGEPLAQPGFLLECLAALKSAGIRTAVDTSGYAPSG
                     ILESAA"
     gene            complement(494..1216)
                     /locus_tag="P5313_11990"
     CDS             complement(494..1216)
                     /locus_tag="P5313_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY81130.1"
                     /translation="MAFSGIQSTPAELLLRERVYDYLKKSLAEGRLSPGSYLNLSALE
                     ADLGLSRTPLRDALLRLEAEGFVTIHSRRGVIVNALDPTAIRDIYQIVGALESAAAEE
                     SRESAGDQTWATMERLNREMKDALSKDDFNEYYSRNLSFHDAFISLSANKELKTTIHT
                     LKERLYDFPRQKSYLREWEIASVREHDEIIRLFRSGDYSGAAAYLRNTHWSYALQEHY
                     VRAYYFKDVIRPDAMRPDTETP"
     gene            1343..2068
                     /locus_tag="P5313_11995"
     CDS             1343..2068
                     /locus_tag="P5313_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY81131.1"
                     /translation="MRTFVFFLYFWLTVACSSPIALILWVLDSAGLHAVRPLTGVLVR
                     LWARSLIALLGVRVRTEGLERIPKGEPVCFVSNHQGNFDIAVILACLPGCVGFITKSQ
                     ALWFPFLNLWILALGGVFLDRDRISKARQALDKGVGRLRSGASMVVFPEGTRSRGPDM
                     GEFHGGSFKLATRSGASIVPISIQGTYHIWEEKGSIVPASVTLKAGEPVPTRGLDPEG
                     RKALPALVRDRIARAQDSTERAG"
     gene            complement(2117..3847)
                     /locus_tag="P5313_12000"
     CDS             complement(2117..3847)
                     /locus_tag="P5313_12000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY81132.1"
                     /translation="MNFTELDLHPDLRKGVEEAGYVSCMPVQAQVFPHAFAGEDIYAQ
                     SQTGTGKTAAFLVSIFQRIMTDPAMRDRKVLILAPTRELAVQILEETRKLGKYLPVSS
                     GAFFGGVGYGPQVDMLKANVQMIIGTPGRIIDLIQQGKMLLKDVGFLVIDEADRMFDM
                     GFIDDLKRLLRYLPPPDKRQTYLFSATLGERVRHLAWDYMREPKEIVIEPENVTVDLV
                     TQEVHHVAASDKFRLLLGILRRENPQSAIIFCNQKYMVEEVARRLNINGITCDFIMGD
                     LPQVRRLEIIRQLKAGTLKILVATDVAARGLDIDALDMVVNYDVPLDTESYVHRIGRT
                     ARAGKSGKAVTLACEKFVYSLPAIEEFIHMRIPVAPFDDSLLADDRSSGMHFGHGRDR
                     GRDVGGRRGDRDSRRGDGRRESRRDGRPGPRQGHDRPSPRPRPAAPAKAVETPASESR
                     APARRPAAADSELYNLPLEERMRLYKQKYGGPEARSEGAEPKKDDGGSRRHRNRRPAA
                     AAGSPPTAPSGGRRDHRPDQKPRDQRPVPAERPRGIPSGKAAASPASADAGQGRKKGL
                     LGFITGLFKK"
     gene            4059..5546
                     /locus_tag="P5313_12005"
     CDS             4059..5546
                     /locus_tag="P5313_12005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRY81133.1"
                     /translation="MQPRTIPRRILNGPAIVALCLVLPAGACSVRVFVDAPQAVSEEA
                     EALRAGLGSPLSRGTPSVRPVGDRHRADIVLHLEYSWSGEGSPGSPESFPIVWRRPYA
                     PAVRIGGPGSSSAELLRDLGRAVSFEDFRSGAMEIRPLESIRPPWVALPVDGRTADQP
                     GYPLFREARVRVEPVGRRGRRHEDSVRAALVNLVANGTIPSDHTPEILWIAAAGDLMT
                     GRGIDHRLLERGADAVFDVQILRVLRDSDLATANLEGTLTSGGVRAEKRFTFRSPPAV
                     AEVLADAGLDILLLANNHSLDRGPEGLSDTRAALEEAGLAGLGAGADIEEAARPYREL
                     RKGQALAVYGAASFPRERSGWDGAAAAAGPGRAGILWLDAEGFNRIRTAFREDTLDLV
                     LIHGGEEWSRTPSREFRRTVTDLVRAGADVVLGSHPHVVQGMDWIDGRPVFWSLGNFV
                     FPGMDGTPGGEEGLLVRAGFLEGRMLYVQTLPLTLSVEGVRAARR"
     gene            complement(5699..7327)
                     /locus_tag="P5313_12010"
     CDS             complement(5699..7327)
                     /locus_tag="P5313_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY81134.1"
                     /translation="MVTVSDLKLSFGDRVLFKDVNLKFTPGNCYGIIGANGAGKSTFL
                     KLLSRELEADSGQISYPSKARMAVLRQEHYQYEDLPILEVVIMGYPKLHRIRKEREDL
                     YAKPDFSEADGLRAGELETEFAELGGWEAEAQAAILLSGLGMAESEHGRLMREIDEGR
                     KVRVLLAQALFGNPDILLLDEPTNGLDLDSISWLEEFLIEFPNIVIVVSHDRHFLNAV
                     CTHTCDFGAIRMYPGNYDFWYRMSRILTQQMKDDKRRREEKIKDLKEFIQRFASNASK
                     SRQATSRKKILDKLDVEDLQPSSRRFPYVGFKPAREPGNNVLSIQGISKTQEGQRILS
                     AFSLEVQRTDKIAFVGREHLAKTALFGILAGEDAAFEGSFSWGQTLSVSYFPKDNAPL
                     FQSDLSIVDWLRQYTESEDETYIRSFLGRMLFSGDEALKPVRVLSGGEKVRCMLSRLM
                     LSEANCLILDEPTNHLDLEAITALNNGLIEFPGVLLFTSHDHEFVNSIASRIVEICPG
                     GVIDRAMPFDDYLADPKIRALRDEYYHGHVIAEI"
     gene            7439..8677
                     /locus_tag="P5313_12015"
     CDS             7439..8677
                     /locus_tag="P5313_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY81135.1"
                     /translation="MPNRNPSKKTRALEDLFLAVLEKRADKAAVEAAKPFSDEALPED
                     IARLVDSAISRGVGFDVLKPGVSKLLNLLYDPLRNHRYPGGEVFLDSLMQENRGAVEL
                     LAEARSAVAAMNASGGSHPEGLARIRSFAERLGALEIHYLKKENILFPAFEARYPEYR
                     CVQLMWSIHDDVRRSLRRLRELCEEADSLLSEINGEIGRLYFNIHAVIFREECIVFPL
                     LAELFDRVSLDAMFEESRSFGFCFLGEDSVRDLVSRSRPKSGFPEEPPSDACPEGTGS
                     PQAGGPELQTDTGVLSFEVMESLFRALPVDMTFVNAAGKVTWFSNSPHRIFPRSPSII
                     GRDVRNCHPRESVDRVVAILEDFRSGRREREAFWIRHRGRFIHIEYFALRDPEGRFLG
                     TLEVSQDLTEKRELEGEKRL"
     gene            complement(8672..9766)
                     /locus_tag="P5313_12020"
     CDS             complement(8672..9766)
                     /locus_tag="P5313_12020"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRY81136.1"
                     /translation="MTEHDRPSRRNEGPVPLILRWIAAALGALTLGSCYVTNQGFRYL
                     SLRARAVGLEKVLSDPEAPEELKDLVRRAERIRTFAISELGLRGTRNYRSLVRLDSDR
                     LATVVSACDSVSFARHLWTYPVVGALPYKGFFEPGEAEREAERLKAKGLDTLIRPVDA
                     FSTLGWFADPLFSNFSRYDDGELAELIIHELAHATVFVKRAEQFNEEFATFVGRRGAR
                     LYISRMYGAQSPELSALDDARADAEAFADFLRGTAELLEAVYSSDRSREEKLAAKSGI
                     LEARAAEFRESALLRFRQEAYRTFAMDRINNAYLDLYRLYEGEPELYAEYLEQRSGGD
                     LARFIREVADLSKSGGDPKDRIRSALGLRL"
     gene            complement(9780..>10229)
                     /locus_tag="P5313_12025"
     CDS             complement(9780..>10229)
                     /locus_tag="P5313_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HRY81137.1"
                     /translation="TESERIFSEVDFSLLVFFSGLFIITRAIEGTALFRAAVSAALPA
                     ASRSPWAFSGASLALSNLVSNVPAVMLLRPMIPSFPDPENAWLLLALSSTFAGNLTLL
                     GSVANLIVAEGAKRRGIELSFGAYLRAGLPITVITAVLGTAWLAAAA"
BASE COUNT         1729 a         3181 c         3424 g         1895 t
ORIGIN      
        1 gcagcgctct ccaggatacc ggacggggcg tacccgctgg tatccacggc ggttcggata
       61 cctgcggact tcaacgcggc caggcactcc agcaggaatc ccggctgggc gaggggttct
      121 cccccggtga aggtgacgcc gcctcccgat tcatcgtaga aggtctcgtc ctgccgggcc
      181 ctaaggagga cctcgtccac ggagaggggg aaacctacga tctgccgggc ttccgcgggg
      241 caggcttccg cgcagagtcc gcaggcgacg caggccgagg gatccgcccc gcgggggcag
      301 accgtccggc aggcaccgca ggcggaggtt cccaggcatc ggtccgggcg gaagatcaat
      361 tcggacttcc ctgctaggcc ctcggggtta tggcaccagg gacaggccag cgggcagcct
      421 ttcaggaaaa aggccgtgcg tatcccgggg ccgtcgtgga tggaatactt tcggacgtcg
      481 aagagcagtc ccgtcatggg gtttccgtgt ccggccgcat agcgtccgga cgtataacgt
      541 ccttgaagta gtaggcacgg acataatgct cctggagggc ataggaccaa tgggtgttcc
      601 taagatacgc ggcggctccc gagtaatccc cggaccggaa cagccggatg atctcgtcgt
      661 gctcccggac cgaggcgatc tcccattccc ggagataact cttctgccgg ggaaagtcgt
      721 acagccgttc cttcaacgta tggatggtgg ttttcaattc cttgttggcg gaaagggaga
      781 tgaaggcatc gtgaaaggac aggttccggg agtagtactc gttaaagtcg tccttcgaca
      841 gggcgtcctt catctcccga ttgagccgct ccatggtcgc ccaggtctga tctcccgcgg
      901 attcccggga ttcctccgcc gcggcgctct ccagggcccc gacgatctga tagatgtcgc
      961 ggatggccgt gggatccagg gcgttcacga tcacgccgcg ccgggagtgg atggtgacga
     1021 aaccttccgc ttccagtcgg aggagggcat cccggagggg agtacgggag agtccgagat
     1081 cggcttccag ggcggagagg ttcaggtagc ttcccgggga gagcctgcct tcggccaggg
     1141 acttcttaag gtaatcgtac accctctccc gaagcaggag ctctgcgggg gtgctctgga
     1201 tcccggaaaa agccatatat gatacttact tgatatatca gatgtgtcaa gggccttttc
     1261 ctattttgga tataccggcg gatacttcgg cttcttccag aggccttgca accgagggaa
     1321 atccggccga ctctatctcc gtatgcgtac cttcgtattc ttcctctact tctggctcac
     1381 cgtcgcgtgc tcgtccccga tcgccctgat cctgtgggtc ctggactccg cggggctcca
     1441 cgctgttcgt ccgcttaccg gagtcctcgt acggctctgg gcccgcagcc tcatagccct
     1501 gctgggagtc cgcgtccgca ccgaggggct ggaacggatc ccgaaggggg aacccgtctg
     1561 tttcgtgtcg aatcaccagg ggaacttcga tatcgccgtg atcctggcct gcctgcccgg
     1621 ctgcgtcggg ttcatcacga agagccaggc cctgtggttc ccgttcctga acctatggat
     1681 cctcgccctc ggaggggtct tcctggaccg tgacaggatc tccaaggccc gccaggctct
     1741 ggacaaggga gtcggtcggc tcaggtccgg ggcctcgatg gtggtgttcc cggaaggcac
     1801 ccgcagccgc ggtccggaca tgggagagtt ccacggaggc agtttcaagc tggccacccg
     1861 gtccggcgcc tccatcgtcc cgatctcgat ccagggcacc taccatattt gggaggagaa
     1921 ggggagcatc gtcccggcct cggtgaccct caaggcgggg gaacctgtcc cgacccgcgg
     1981 actggatccg gagggcagga aggccctgcc ggcactggtc cgggaccgta tcgcgagagc
     2041 ccaggattct acggaacggg caggatgagg aggggggtgt ccaaaaagct cggtttgctt
     2101 tttggacacc cggccgtcat ttcttgaaca gaccggttat gaatcccagc aggcccttct
     2161 tgcggccctg cccggcgtcc gcggatgccg gggacgccgc ggccttaccg gaaggtatcc
     2221 cccgcggacg ttcggccgga accgggcgct gatcccgggg cttctgatcg ggcctgtgat
     2281 ccctgcggcc gccggacggg gctgtcggag gggaccccgc cgccgcggcg gggcggcgat
     2341 tcctgtgccg ccgggagccg ccgtcgtcct tcttgggttc ggccccctcg gaccgagcct
     2401 ccggtccgcc gtacttctgc ttatatagtc gcatgcgctc ttccagggga aggttgtaca
     2461 gttccgagtc ggcggccgcc ggtctccgag ccggggcacg agattccgag gccggggttt
     2521 cgacggcttt cgcgggtgcg gcgggccgtg gtcgggggga gggcctgtca tggccctgtc
     2581 ggggcccggg ccttccgtcc ctgcgggatt ccctgcggcc gtctccccgt cgggagtccc
     2641 gatcgcccct gcgtccgccg acgtcccggc cgcggtcccg tccgtgcccg aaatgcatgc
     2701 ccgagctccg gtcgtccgcc aggagggaat cgtcgaaggg ggcgacgggg atcctcatat
     2761 ggatgaactc ctcgatcgcc gggaggctgt acacgaattt ctcgcaggcc agggtgacgg
     2821 ccttgcccga cttgcccgcc cgggcggtac ggccgatccg gtggacatag gactccgtgt
     2881 ccaggggcac gtcgtagttg accaccatgt ccagggcgtc gatgtccagc ccccgggcgg
     2941 cgacgtccgt ggcgacaagg atcttcaggg taccggcctt gagctggcgg atgatctcga
     3001 gacgccgtac ctggggaagg tcgcccatga tgaaatcgca ggtgattccg ttgatgttca
     3061 acctccgggc cacctcctcg accatgtact tctggttgca gaagatgatg gcgctttggg
     3121 ggttttcccg ccgaaggatg cccagcagga gccggaactt gtcgctggcg gccacatggt
     3181 ggacttcctg ggtgaccaga tccaccgtga cgttctcggg ctcgatcacg atctccttgg
     3241 gttcccgcat atagtcccag gcaaggtgcc ggacgcgttc cccgagggtg gcggaaaaca
     3301 ggtaggtctg ccgtttgtcc ggcgggggga ggtatcgcag caaccgcttg aggtcgtcga
     3361 tgaaccccat gtcgaacatg cggtccgcct cgtcgatgac caggaacccc acgtccttga
     3421 ggagcatctt gccctgctgg atcaggtcga tgatccggcc cggggtcccg atgatcatct
     3481 ggacgttggc cttgagcatg tctacctggg ggccgtatcc gacacccccg aagaaggctc
     3541 cggagcttac ggggagatac ttgccgagtt tcctcgtctc ctccaggatc tggaccgcca
     3601 gctcccgagt gggagccagg atcagcacct tccggtcccg catcgcgggg tcggtcatga
     3661 ttcgctggaa aatggagacg aggaaggcgg cggtcttgcc ggtgcccgtc tgggactggg
     3721 cgtagatgtc ttccccggcg aaggcgtggg ggaagacctg ggcttgtacg ggcatgcagg
     3781 agacgtatcc cgcctcctcg acgccctttc tgagatccgg atgcaggtcc aattccgtga
     3841 aattcatcgt gtgtccattc ttgtggaaag agtctttctc aaagaataca ggaaacttcc
     3901 ctctttgtta agggatgctt ccgtatagca tcgttttctg tcgtcaggga gccgcaggtg
     3961 gaagaccaag attccggttt cgaagccggc gcatggggta tactcccctg cagaatgcgg
     4021 gtcggcctcg gggccggcgg tgcgaaaagg cggagcgcgt gcaacctcgt accatcccca
     4081 ggagaatcct caacgggccc gcgatcgtcg ccctctgcct cgtcctcccg gcgggcgcct
     4141 gctcggtccg ggtcttcgtg gacgcgcccc aagccgtttc tgaggaagcg gaagccctgc
     4201 gagccggatt gggatcgccg ctttcccggg gcactccttc tgtccggcct gtaggggacc
     4261 gacatcgggc ggatatcgtc ctgcacctgg agtattcctg gtccggagag ggatccccgg
     4321 gatccccgga gtcttttccg atcgtatggc gtcgtcccta tgcccccgcc gtccggatcg
     4381 gagggccggg atcttcgtcg gcagagctgc ttcgggacct gggccgggcc gtatccttcg
     4441 aggatttccg ctccggtgcg atggaaatcc gccccctgga atccatccgg cctccctggg
     4501 tcgcccttcc ggtcgacggg aggaccgccg accaacccgg gtatcccctg ttccgggagg
     4561 cgagggtccg ggtggaaccc gtcggtcgcc ggggccggag gcacgaggac tcggtgcgtg
     4621 cggccctggt gaatctcgtc gcaaacggga cgattccctc agatcataca ccggagatac
     4681 tctggatagc ggcggcgggg gacctgatga ccggccgggg gatcgaccat cggctcctgg
     4741 agaggggagc cgatgccgta ttcgacgtcc agatcctccg ggtcctgcgg gattcggacc
     4801 tcgcgacggc caacctggag gggaccttga cctccggggg agtgcgcgcc gaaaagcggt
     4861 tcaccttccg gtccccgccc gccgtggcgg aagtcctggc ggacgctggc ctggatatcc
     4921 tgctcctggc gaacaaccac agccttgatc gggggccgga gggtctatcg gacacccggg
     4981 ccgccttgga ggaagcgggc ctggccggcc tcggcgcggg agccgatatc gaggaggctg
     5041 cccggccgta ccgggagctc aggaagggac aagccctcgc cgtgtacgga gcggcttcct
     5101 ttccgaggga acggagcggc tgggacggag ctgccgccgc ggccggtccc ggccgggccg
     5161 ggatcctctg gctggacgcg gaagggttta accgcatccg cacggccttc cgggaagaca
     5221 ccctggacct cgtgcttatt cacggcgggg aggaatggtc ccggacaccg tcccgggagt
     5281 tccgccgcac cgtgacggac ctcgtccggg ccggggcgga cgtggtcttg ggatcccacc
     5341 cccacgtggt ccaggggatg gactggatcg acgggcgtcc ggtcttctgg tccctgggga
     5401 atttcgtctt tccggggatg gacggcaccc ccggagggga ggaaggactc ctggtccggg
     5461 cgggattcct ggagggccgt atgctctacg tacagaccct gccactaacc ctgtccgtgg
     5521 aaggtgtccg cgccgcacgg cgatagtccc cggtcccgaa agtccaagac agaaggttga
     5581 ccgcggccag ggaggcatac actctgaccc agcgaagttg cagatggccg cgaatccacg
     5641 gattttcggg cttttgtgaa accgtttata gaaatgctcg aagcatttct ataaacggct
     5701 agatttccgc gatcacgtga ccgtggtagt actcgtcccg gagggcgcgg atcttggggt
     5761 ccgcgaggta gtcgtcgaag ggcatggccc ggtcgatgac tcctcctggg cagatctcca
     5821 cgatccggct ggctatggag ttaacgaact cgtggtcgtg gctggtgaac aggagaaccc
     5881 ccgggaattc gatcaatccg ttgttcaggg ccgtgatggc ctcgagatcc aggtggttgg
     5941 tcggctcgtc caggatcagg cagttggctt ccgagagcat gagacgggaa agcatgcagc
     6001 ggaccttctc ccctccggac aggacccgca cgggcttgag ggcctcgtcc ccggaaaaga
     6061 gcatccgacc caggaaggag cggatgtagg tctcgtcctc gctttccgtg tactgcctga
     6121 gccagtccac gatggacagg tcggactgga agaggggggc gttgtccttg gggaaatagg
     6181 agaccgaaag ggtctgcccc caagagaaag agccctcgaa agccgcatcc tcgcccgcca
     6241 ggatcccgaa cagggccgtc ttggccaggt gttcccggcc cacgaaggcg atcttgtccg
     6301 tgcgctggac ctccagggag aaagcggaaa ggatcctctg tccctcctgg gtcttcgaga
     6361 taccctggat ggacaggacg ttgttgcccg gttcccgggc gggcttgaac ccgacgtagg
     6421 gaaagcgccg gctcgagggc tggaggtcct cgacgtccag cttgtccagg atcttcttcc
     6481 gggacgtggc ctggcggctc ttggaggcgt tggacgcgaa gcgctggatg aactccttga
     6541 ggtccttgat cttctcttcc ctgcgccgct tgtcgtcctt catctgttgg gtcaggatcc
     6601 ggctcatacg gtaccagaaa tcgtagttgc ccgggtacat ccggatggcg ccgaaatcgc
     6661 aggtgtgggt gcagaccgcg ttcaggaagt gccggtcgtg ggacacgacg atgacgatgt
     6721 tggggaactc gatcaggaac tcctccagcc aggagatgga gtccaggtcc aggccgttcg
     6781 tgggctcgtc caggaggagg atgtccggat tcccgaagag ggcctgggcc aggaggaccc
     6841 ggaccttccg gccctcgtcg atctcccgca tgagcctgcc gtgctccgat tccgccattc
     6901 ccagtcccga cagcaggatg gccgcctggg cctcggcctc ccaaccgccg agctccgcga
     6961 actcggtctc caactcgccg gcccgcaatc cgtcggcctc cgagaagtcg ggcttggcgt
     7021 agaggtcctc gcgttccttc cgtatcctgt ggagcttggg ataccccatg atcaccacct
     7081 ccaggatcgg cagatcctcg tattggtaat gctcctgccg gagcaccgcc atccgggcct
     7141 tggaggggta ggatatctga ccggaatcgg cttccagttc ccgggacagc agcttcagga
     7201 aggtggactt cccggcccca ttggccccga tgatgccgta gcaatttccg ggggtgaatt
     7261 tcaggttcac gtccttgaag aggacgcggt cgccgaagga gagcttcaga tcgctgacgg
     7321 taaccacgga atatcctttt gcgcgcgttc gtgcgcgggt ggaattcgaa tgtcctgtgt
     7381 tgtttgataa tcccggaaaa attggaaaat tgcaagccgg gccgttacac ttctgctcat
     7441 gccgaaccgg aatccatcga aaaagacccg cgccctggag gatttgttcc tggcggtgct
     7501 cgagaagagg gccgacaagg ccgccgtgga ggccgccaag cccttttccg acgaggccct
     7561 tccggaggac atcgcccggc tggtcgactc cgccatttcc cggggggtcg gcttcgatgt
     7621 cctcaagccc ggtgtgtcca agctcctgaa cctgctgtac gatccgctcc ggaaccaccg
     7681 gtaccccggc ggggaggtct tcctggattc cctcatgcag gagaaccggg gagccgtcga
     7741 gcttctggcg gaggcccgat ccgccgtggc ggccatgaac gcctccggcg gttcccatcc
     7801 cgaggggctc gcccggatcc gatccttcgc ggaacgtctc ggggccctgg agatccacta
     7861 cctcaagaag gagaacatcc tgttcccggc cttcgaggcc cggtatccgg agtaccgctg
     7921 cgtccagttg atgtggtcca tccacgacga tgtccggagg tccctgcgcc gtttgcggga
     7981 gctctgcgag gaggccgact ccctgctgtc cgagatcaac ggggagatag gacggctcta
     8041 tttcaacatc cacgcggtca tcttccggga ggagtgcatc gtgtttccgc ttcttgcgga
     8101 gctgttcgac cgggtgtccc tggacgcgat gttcgaggaa tcccggtcct tcggattctg
     8161 cttcctgggg gaggactccg tccgggacct ggtctcacgc tcccggccga aatccggatt
     8221 ccctgaggag ccgccctccg acgcctgtcc ggaagggacc ggatccccgc aggccggggg
     8281 accggagctt caaacggata cgggcgtcct ttcctttgaa gtcatggaat ccctcttcag
     8341 ggcactgccc gtggacatga ccttcgtgaa cgcggcggga aaggtgacct ggttttccaa
     8401 cagtccccac cggatatttc ccaggagccc ctcgatcatc ggccgggacg tccggaactg
     8461 ccatccccgg gaaagcgtcg accgtgtcgt cgccatcctg gaggatttcc gatcgggtcg
     8521 gagggaacgg gaggcgttct ggatccggca ccggggacgg ttcatccata tcgagtactt
     8581 cgccctgcgc gatccggagg gacggttcct ggggaccctg gaagtcagcc aggacctgac
     8641 ggaaaaacgg gaactcgagg gggagaagag gctataagcg gaggccgagg gccgaacgga
     8701 tccggtcctt gggatccccg ccggacttcg acaggtccgc gacctcccgg atgaaccgcg
     8761 ccagatcccc gccggatcgt tgctccaggt attccgcgta cagctccggc tccccctcgt
     8821 acagtcggta aagatccagg tatgcgttgt tgatcctgtc catggcgaag gtcctgtacg
     8881 cctcctgccg gaaccgaagg agggcggatt cccggaactc cgcggcccgg gcttcgagga
     8941 tcccggactt ggcggccaac ttttcctccc ggcttcggtc ggacgagtag acggcctcca
     9001 ggagctccgc ggtaccccgg agaaaatccg cgaaggcctc cgcgtccgca cgggcgtcgt
     9061 ccagggccga gagctccgga ctctgcgctc cgtacatccg ggagatgtac agccgggctc
     9121 cccggcgtcc gacgaaggtc gcgaactcct cgttgaactg ctccgcccgc ttcacgaaga
     9181 ccgtggcgtg ggcgagctcg tggatgatca actccgccag ctcgccgtcg tcgtaccggg
     9241 agaaattgga aaacagggga tccgcgaacc agcccagggt gctgaaggcg tccacgggcc
     9301 ggatcagtgt atccaggcct ttcgccttca gccgctcggc ctcgcgttcc gcctccccgg
     9361 gctcgaagaa ccccttgtag gggagggcgc ccaccacggg gtaggtccag aggtggcgcg
     9421 cgaaggacac ggaatcgcag gcggatacta cggtggccag acggtcggaa tccaggcgga
     9481 ccagggatcg gtagttccgg gtgccccgca ggcccagttc cgagatcgcg aaggtgcgga
     9541 tcctctccgc cctccgcacg aggtccttga gttcctccgg ggcttccgga tcggaaagca
     9601 ccttctccag gccgaccgcc cgggcgcgga gggaaaggta cctgaacccc tgattcgtca
     9661 cgtagcagga gcccagggtg agagctccca gggcggccgc gatccagcgc aggatcagag
     9721 gtacggggcc ctcgttcctg cggctagggc ggtcgtgttc cgtcatcaga agtctcctct
     9781 cacgccgcgg cggcgagcca cgcggtgccc aggacggcgg tgatgaccgt gatgggcagt
     9841 cccgcccgga gataggcgcc gaaggagagc tcgatgcccc ttcgtttggc cccctccgcc
     9901 acgatcaggt tggccacgga gcccaggagg gtcaggttgc ccgcgaaggt gctggacagg
     9961 gccagaagga gccaagcgtt ttccggatcc ggaaaggaag ggatcatggg ccgcaggagc
    10021 atcaccgcgg ggacgttgga gaccaggttt gagagggcca gggaggcccc ggagaaggcc
    10081 cagggggagc gggacgccgc gggaagggcg gcggaaacgg cggcccggaa taaggcggtc
    10141 ccctcgatgg cccgggtgat gatgaagagc cccgagaaga atacgagcag ggagaagtcc
    10201 acctccgaga agatcctctc gctctcggt
//
LOCUS       DAOTEI010000130        10205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4622_length_10205_cov_5.234778, whole genome shotgun sequence.
ACCESSION   DAOTEI010000130 DAOTEI010000000
VERSION     DAOTEI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4622_length_10205_cov_5.234778"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1235)
                     /locus_tag="P5313_12030"
     CDS             complement(27..1235)
                     /locus_tag="P5313_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY81138.1"
                     /translation="MRRFLERALAKAPRMNEEQIRRLLDILADENERMGTVLDSMLDG
                     IVVCDSAHRPTLFNKSAERLLPLAYLDVYDRPVWESVSDEEIAGFLRETLESEETRMD
                     REFALDAKGSTRIVSITISPLVREQRIRGNLIHVEEVTDKRRKEAQLRRAENLASLTT
                     LAAGVAHEIKNPLGSLSIHVQLMRKALKASCDERSGTIHRYLDVVDEEIDRLNRIVVD
                     FLFAVRPMDIQPRDTDLNGLLSDLLDFLAPEIERAEVRLERDLSPAVPRLRLDPRYMK
                     QAVLNLVQNALAAMPRGGKLTLSTGRGDDGILLTVADTGTGIPEENLPKIFEPYFTTK
                     ETGTGLGLTLTFKIVKEHGGEIAVRSREGEGTAFTITLPLPPAERRLLSHDSAAGERT
                     PESRTPGEAS"
     gene            complement(1232..2521)
                     /locus_tag="P5313_12035"
     CDS             complement(1232..2521)
                     /locus_tag="P5313_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HRY81139.1"
                     /translation="MYENLIGQEEVVRILRSDLERKELPPSLLFAGPENAGKLTAALE
                     TARALSCETGAAAWNCPCPSCERHRTLSHPDLLLMGPRSFRAEIKAAGEALGRSPNRA
                     ARFLFYRAVRKLTRRFDPALFEGEESRLSKASALLQTLDELLESAGPDAEDAGQAPGE
                     SAQAAAKALPVADKLEALLPDALPIFQVRAVERWARLAPYGRVKVAVIENAERMLDGA
                     RNALLKILEEPPATSRFLLLSSRPSALMPTILSRVRTYPFRPRDARASREILAKVFRA
                     APGEADLSLDEYLAARKPLPESATDEYAFRFLTAVCYQASREGVFIPDPLLDLSGDAP
                     DPTVHKAVSDISAATKGFGSGDDSLSYLFPAFLRSLARLVRSRARASEPRTDAILALW
                     AAAIRDAQVRYETYNLSPPALAERLAEALAESLRSVP"
     gene            complement(2522..2986)
                     /locus_tag="P5313_12040"
     CDS             complement(2522..2986)
                     /locus_tag="P5313_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRY81140.1"
                     /translation="MNALDLVLFVVILLFALRCYFRGFVAELLSMAAVILGVFLAILF
                     YRPAGELLAKYLALESFTEVLGFAVVFLLVFLLIKILQGVLRSVIENINLQNVDRGLG
                     FLLGAAEGLLLSSLLLMLMRLQPVLDLSALLEGSFLGKNLLPVLVSAARLGA"
     gene            complement(2983..4143)
                     /locus_tag="P5313_12045"
     CDS             complement(2983..4143)
                     /locus_tag="P5313_12045"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRY81141.1"
                     /translation="MRGGSETPGSPDVLAFTGGGTGGHIYPGLAVADRLRELLSVRGR
                     SVRFVWLGSTKDSDRAAVEAAGLEFIPIPSGKLRRELSVENLADAFRVLAGYGASRRV
                     LSKLRPRLLFSKGGYVSVPPCRAAAALGIPVFTHESDFSPGLATRLNAGIAETVFVSY
                     EETRDAFPPARRPSVVVSGQPIRAAIRSGDPAAGRSRAGFPEGLPLILVIGGSQGARR
                     LNDLVAAALPELLPKACVIHQTGPGNPGVPREALPPGLEGRYRSFEYLRGELPDLLAA
                     ASIYVGRAGAGSLWECAAAGLPMVLVPLAGTGTRGDQVENARWFAARRAARVLEGRDA
                     EDPSALVSACLGFLEDAEGLRAASRAARELGARDAADIIARAIADRAWGNPS"
     gene            complement(4140..5723)
                     /gene="pgi"
                     /locus_tag="P5313_12050"
     CDS             complement(4140..5723)
                     /gene="pgi"
                     /locus_tag="P5313_12050"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625632.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRY81142.1"
                     /translation="MVQFRNLDESPAFRALLGQGPSDLRAALTAEAFETRRADAGGGL
                     TFSWAASDAGDETVSLLGALAAEQELVEKYRLLASGHRMNTGEDRMVLHHLTRGEPAG
                     KVVRDGRDFRAFYEGERLRAADFARRIHSGSLTGSTGKRFRTAVQIGIGGSDLGPRAL
                     FLALETWARAKGRTRMAARFLSNVDPDDANGVFFDLDPETTLFILVSKSGTTQETLAN
                     ETLARDFLRRRGPAGLDPSRHIVAVTSETSPLARNPGYLESFYIDDFIGGRYSSTSAV
                     GGVVLSLAFGPETFEELLRGAREADERALTPDLLRNPALLDALLGIFERNVLGRPCVA
                     VLPYSQALSRFPAHLQQLDMESNGKQVNRDGHPVVYSTGPVVFGEPGTNGQHSFYQLL
                     HQGTDIVPLEFIGFAENQGDGDLEVEGSTSRAKLNANLAAQVVAFAKGQDSGNPNKRF
                     PGGRPSTLLYGRTLDPRALGALLAHFENKVMFQGFAWNLNSFDQEGVQLGKVLTKQVL
                     AGGPLDGALEALSRALGVR"
     gene            5965..7161
                     /locus_tag="P5313_12055"
     CDS             5965..7161
                     /locus_tag="P5313_12055"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY81143.1"
                     /translation="MNLGISLRLQGAGKSAAAKVKEFRKDSLWMDLPARSGKRFLPWE
                     LLSAYFDFHGKDMTFSSKVLKQEGDFLVLAYPVRLLRSPARRHPRVPCPRGFALEITL
                     QNETVRLDYPQSGEYSDVSLPDLHEGFDISSLNTLIESFRQRSSRMASESRVVLFRDR
                     FPQGVEELMLSRFGRTLFIPSTRSPLPSADPYPEPRIITAQMAQDYEGPEGIVDGSRF
                     DHAMVSKIGRGINAEVWCPILYFQYVVGYVYLANKTDRPVSMDFSVVDCAWEFSRVLA
                     FYLKTNDYFKTAQDGEPVSHTAGIVDLSASGCLLSIPHTALAMRIKTGALLDLRLDYP
                     EGSLQVRGKVVRRFDDKDGAYYGVAFQDLESEDLRVLFPKIYEMSYPDSQEDAGEARL
                     HAGGSA"
     gene            complement(7201..8967)
                     /locus_tag="P5313_12060"
     CDS             complement(7201..8967)
                     /locus_tag="P5313_12060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRY81144.1"
                     /translation="MLDPASESIRRQDPVSAAAETLFGVRYLYPYQRLVVANILDAFP
                     GAAPSPGDLGAVPDTAGTTPEGPDRLRQVVLLPTGYGKSLCFQLPAVFLPGLTLAVYP
                     LRSLMADQARRLRERGLPCAVLQGGRSPEERRAAEEAVRSGRARIALANPEVLRTESV
                     LALLRSVGVAHAVVDEAHCVSEWGDDFRPAYVELGSVLRALDPPAVTAFTATASQGIL
                     ARVAEVLFGDEPWRLVSGNPDRPNLSYRCAPTLSRSHTLERLARTLPKPLLVFCASRA
                     GTQITAERLARALDTREVRFYHAGMTKEERKAVEDWFLPSRDGVLTATCAFGMGIDKP
                     DIRSVIHADPPPSAESYLQESGRAGRDGRPSTAVLIVRPSDRDLLNRDSDPVRRRRRA
                     AVLDYAEGSTRCRRETLLALLGSEAEGCSGCDVCSGSAVPEPEGRREILRFAAANSGR
                     WTVSEASRLLAGIAGPCAWAGALLGWEAGEAAEALSYLAGSGAIEVPRRGAWKGRVRP
                     AARRRWFGPRPAPGAGAAATPRVPPEAHPSPKPEPERPGRPAFGAGGIGIPRACAEPA
                     PRIGRRSRLPGRPRGRPGSPTA"
     gene            complement(9020..9664)
                     /gene="nth"
                     /locus_tag="P5313_12065"
     CDS             complement(9020..9664)
                     /gene="nth"
                     /locus_tag="P5313_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRY81145.1"
                     /translation="METSPFEVERARSLRLALTALWPRAEPLLAYDDCFELLVAVVLS
                     AQTTDDQVNRVTPGLFRRWPDPGRLAEADLAELEDCVHSLGFYRTKARHLIDLSRILV
                     ERFSGRVPERMEELLELPGVGRKTANLVASACFGRPGIIADTHFLRVCHRLGLTENRD
                     PVAAERRIEALIEPEYRTAFSHAVNRHGKYVCTARRPDCPGCAVRELCPSADTA"
     gene            9782..>10205
                     /locus_tag="P5313_12070"
     CDS             9782..>10205
                     /locus_tag="P5313_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81146.1"
                     /translation="MKRILIASILIAMALAAFPAFSQEAKPADPLATQIKSPSDMYPF
                     RLDVVKVFQHSLGFRVVYRVGQADFADAYIPIEWFKAGGKAELIRSNDPSHPYMVIYY
                     REGKFSHVRLFVKDFPKDPSWGILEPGPDVASKFKVEEI"
BASE COUNT         1625 a         3382 c         3602 g         1596 t
ORIGIN      
        1 gtcgtcggcg accaggatgg tgaatttcac gatgcctccc ccggagtccg ggattccggc
       61 gtccgttccc cggccgcgga atcgtgggac aagagccggc gctccgcggg cggaagcggc
      121 agggtgatcg tgaacgccgt cccctcgccc tcccgggaac ggaccgcgat ctcgcccccg
      181 tgctccttga cgatcttgaa ggtaagggtg agccccagcc ccgtgcccgt ctccttggtg
      241 gtgaagtagg gttcgaagat tttcggcagg ttctcctccg ggatacccgt cccggtatcc
      301 gccacggtca ggaggatgcc gtcatccccg cgtcccgtgc tcagggtcag cttccctccc
      361 cgaggcatgg cggccagggc gttctggacc aggttgagga ccgcctgctt catgtaccgg
      421 gggtccagtc ggaggcgggg gactgcgggg gacaggtccc gctccagccg gacctcggca
      481 cgctcgatct ccggtgccag gaagtccagg aggtcggaca gaagcccgtt gaggtccgta
      541 tctcggggtt ggatgtccat gggccggacc gcgaagagga aatccaccac gatccggttc
      601 agtcggtcga tctcctcgtc caccacgtcc aggtaccgat ggatggtccc ggaccgctcg
      661 tcgcaggagg ccttcagggc cttccgcatc agttggacgt gaatcgacag ggaacccagg
      721 gggttcttga tctcgtgggc gactcccgcg gccagggtcg tcagggacgc caggttttcc
      781 gccctgcgga gctgggcctc cttacggcgc ttgtccgtca cctcctcgac gtggatcagg
      841 tttccccgga tccgctgttc cctcaccagg ggacttatgg tgatcgagac gatccgggtc
      901 gagcccttcg cgtccagggc gaattcccgg tccatccggg tttcctcgct ctccagggtt
      961 tcccggagga atccggcgat ctcctcgtcc gagacgctct cccagacggg ccggtcgtac
     1021 acgtccaggt aggccagggg cagcaacctc tccgcggact tgttgaaaag ggtgggacgg
     1081 tgggcggagt cgcacacgac gatgccgtcc agcatggagt ccaataccgt gcccatccgc
     1141 tcgttctcgt ccgccaggat gtccaggagt ctgcggatct gctcctcgtt catccgcggg
     1201 gccttggcca gggcccgttc caggaacctt ctcacgggac gctccggagg gattcggcca
     1261 gggcttccgc gaggcgttcc gccagggcgg ggggcgagag gttgtaggtc tcgtaccgga
     1321 cctgggcgtc ccggatggcc gccgcccaga gggccaggat cgcgtcggtc cggggttccg
     1381 aggcgcgggc ccgggagcgg accagccggg ccaggctgcg gaggaaggcg gggaagaggt
     1441 aggaaagtga gtcgtccccg gacccgaagc ccttggtggc cgcggagatg tcggaaaccg
     1501 ccttgtggac ggtcgggtcc ggcgcgtcgc cggagaggtc cagcagggga tcggggatga
     1561 agaccccctc ccgggaggcc tggtagcaga ccgcggtcag gaaccggaag gcgtactcgt
     1621 ccgtcgcgga ctcgggcagg ggtttccggg ccgcgaggta ctcgtccagg gagagatccg
     1681 cttcccccgg ggcggcgcgg aacaccttgg ccaggatctc ccgggatgcc cgggcatccc
     1741 gggggcggaa cggataggtc cgtacccggg aaaggatggt gggcatgagg gcggaggggc
     1801 gggaggacag gagcaggaag cgggaggtgg cgggaggctc ctccaggatc ttgagcaggg
     1861 cgttgcgggc cccgtccagc atcctctcgg cgttctcgat caccgcgacc ttgacccgtc
     1921 cgtagggcgc cagccgcgcc cagcgttcca cggcccggac ctggaagatg ggcagggcgt
     1981 cgggaagcag ggcctccaat ttgtccgcga ccgggagggc cttggccgcc gcctgggcgg
     2041 attccccggg ggcttgtccc gcgtcctccg cgtccggccc ggcggactcc aggagttcgt
     2101 ccagggtctg gagcagggcg gacgccttgg aaaggcggga ctcctcgccc tcgaagagcg
     2161 cggggtcgaa gcggcgggtg agcttccgga ccgcccgata gaagaggaag cgtgcggccc
     2221 ggttggggct ccggccgagg gcctccccgg cggccttgat ctccgcccgg aaggaccggg
     2281 gtcccatcag gaggaggtcc ggatgcgaga gggtccggtg gcgctcgcag gaggggcagg
     2341 gacagttcca ggccgcggcg ccggtctcgc aggacagggc tcgggcggtc tccagggcgg
     2401 ccgtgagctt gcccgcgttt tccggccccg cgaagagcag ggacgggggg agctccttcc
     2461 gctccaggtc ggaacggagg atcctcacga cctcctcctg gccgatcagg ttctcgtaca
     2521 cctaggcccc cagccgggcg gcggacacga ggacgggcag gaggttcttc cccaggaagc
     2581 tgccctccag cagggccgag aggtccagga ccggctgaag ccgcataagc atgagcagga
     2641 gggaggacag aagcagccct tccgccgccc ccaggaggaa gcccagaccc cggtccacgt
     2701 tctgcaggtt gatgttctcg atgacgctcc gcaggacacc ctgcaggatc ttgatgagca
     2761 ggaagaccag caggaagacg acggcgaagc ccaggacctc ggtgaaggac tccagggcca
     2821 ggtacttggc caggagttcc cccgcggggc ggtagaagag gatggccagg aacaccccca
     2881 ggatgacggc ggccatggag agaagttcgg ccacgaagcc ccggaagtag cagcgcagcg
     2941 cgaagaggag gatgactacg aacagcacca gatccaaggc gttcatgacg ggttccccca
     3001 ggctcgatcg gctatggctc gggcaatgat gtccgccgcg tcccgggccc ccagttcccg
     3061 ggccgcacgg gacgccgccc ggagcccctc ggcgtcttcc aggaatccga ggcaggcgga
     3121 taccagggcg gaggggtctt cggcgtcccg gccttcgagc acccgcgcgg cgcgccgggc
     3181 cgcgaaccag cgggcgttct cgacctggtc tccccgggtg ccggtccccg ccagggggac
     3241 caggaccatg ggcagccccg cggcggcgca ttcccagagg gaacccgctc cggcgcgccc
     3301 cacgtagatg ctggcggcgg ccaggaggtc cggaagttcc ccccgcaggt actcgaagga
     3361 gcggtaccgc ccttccagtc ccgggggcag ggcttcccgc gggactcccg ggttgccggg
     3421 tcccgtctgg tggatcacgc aggccttcgg aaggagttcc ggcagggccg ccgccaccag
     3481 gtcgttcagg cgccgggcgc cctggcttcc cccgatcacg aggatcaggg gaagtccctc
     3541 ggggaaaccg gcccgggagc gcccggcggc cggatccccg gaccggatgg ccgcccggat
     3601 cggctggccg gagacgacca cggagggtct ccgggcgggc gggaaggcgt ccctcgtctc
     3661 ctcgtagctc acgaagacgg tttccgcgat ccccgcgttg aggcgggtgg ccagacccgg
     3721 ggagaagtcg gactcgtgag tgaagacggg tatgcccaag gctgcggcgg cccggcaggg
     3781 cgggacggag acgtatccgc ccttggagaa caacaggcgc ggccgcagct ttgaaaggac
     3841 gcgccgggaa gccccgtatc ccgcgaggac gcggaaggcg tccgccaggt tttccacgga
     3901 aagttcccgg cggagcttgc ccgagggaat cgggatgaac tccaggcccg ccgcctccac
     3961 cgccgcccgg tcgctgtcct tcgtggaacc cagccagacg aagcggacgc tacggccccg
     4021 aacggacagg agttcccgga gccggtccgc aacggccaga cccgggtaga tgtgccctcc
     4081 cgtgccgccc ccggtgaagg ccaggacgtc cgggcttccg ggggtttcgc tccctcccct
     4141 catcgtaccc ccagggcgcg ggacagggct tccagggccc cgtcgagggg gcctccggcc
     4201 aggacctgct tggtcaggac cttgcccagc tggactccct cctggtcgaa gctgttcagg
     4261 ttccaggcga agccctggaa catcaccttg ttctcgaagt gggccaggag ggctcccagg
     4321 gcccggggat ccagggtccg gccgtacagg agggtggagg ggcggcctcc ggggaaccgc
     4381 ttgttcggat tgccgctgtc ctgtcccttc gcgaacgcga ccacctgggc ggccaggttg
     4441 gcgttcaact tggcccggct tgtggaacct tcgacctcga ggtccccgtc cccctggttc
     4501 tccgcgaaac cgatgaactc caggggcacg atgtccgtgc cctggtgcag gagttggtag
     4561 aaggagtgct ggccgttcgt cccgggttcg ccgaatacca cgggcccggt ggaatagacc
     4621 accggatggc cgtcccggtt tacctgcttg ccgttggatt ccatgtccag ctgctggagg
     4681 tgggcgggga agcgggagag ggcctgcgag tagggcagga cggccacgca gggtcggccc
     4741 aggacgttcc gctcgaagat cccgagcagg gcgtccagga gggcgggatt ccggaggagg
     4801 tccggggtca gggcccgttc gtccgcctcc cgggcgcccc gcaggagctc ctcgaaggtc
     4861 tccgggccga aagccaggga caggacgacc cctcccaccg cggaggtgga ggaataccgg
     4921 ccgccgatga agtcgtcgat gtagaaggat tccaggtagc cggggttccg tgccaggggg
     4981 ctggtctcgc tggtcacggc gacgatgtgg cgggaaggat cgagtcccgc gggtccccgc
     5041 cgccggagga agtcccgggc cagggtctcg ttggccaggg tctcctgggt ggtcccgctc
     5101 ttggacacca ggatgaagag ggtggtctcc gggtccaggt cgaagaacac cccgttcgcg
     5161 tcgtcggggt ccacgttgct caggaaccgg gcggccatcc gcgtccggcc cttggcccgt
     5221 gcccaggtct ccagggccag gaagagggcc cggggaccca ggtcggatcc gccgattccg
     5281 atctgcacgg cggtacggaa tcgcttgccc gtggagcccg tcagcgaccc cgaatggatc
     5341 cgccgggcga aatccgcggc ccgcaggcgc tccccctcgt agaaggcgcg gaagtcccgg
     5401 ccgtcccgga ccaccttccc cgcgggttcc ccccgggtca ggtggtggag caccatacgg
     5461 tcctcgccgg tgttcatgcg gtgccccgag gccaggaggc ggtacttctc cacgagttcc
     5521 tgctccgccg cgagggcgcc gagcagggaa accgtctcgt ctcccgcgtc cgaggcggcc
     5581 caggaaaagg tcaatccccc gcccgcgtcc gcccttcggg tctcgaaggc ttccgcggtc
     5641 aaggcggcac ggagatccga cggaccttgg ccgagaaggg cccggaacgc cggggactcg
     5701 tcgagattcc ggaattgaac catggggacc tcctggtcga aacggccccg ggggctgttt
     5761 cctttcgata cgctcatgat agccgggacg ggacggatct ttcaaggccg agctttccgc
     5821 gcgggtacgg ctttccggac ttttgacccg aaccgggttc cctccgcact ttcttcctcc
     5881 ggaaacgcat ggtatactcg ggcgggggct gtgatggccg aactcgtaaa tcatgtagaa
     5941 acggaattca ttcttaagga agccatgaac ctcggcattt cgctccgtct acagggagcc
     6001 gggaagagcg ccgcggccaa ggtcaaggaa ttccgcaagg attccctctg gatggatctt
     6061 ccggcccgga gcggcaagag gttccttccc tgggagctct tgtccgcgta tttcgatttc
     6121 cacggcaagg acatgacctt cagctccaag gtcctcaagc aggaagggga cttcctggtc
     6181 ctggcctatc ccgtccgtct gcttcgctcc ccggcccggc ggcacccccg ggtgccctgc
     6241 ccccggggat tcgccttgga gatcaccctg cagaacgaga cggtccggct ggactatccc
     6301 cagagcgggg agtattcgga tgtctccctg ccggatctgc acgaagggtt cgacatcagt
     6361 tccctgaaca cattgatcga aagtttccgc cagcgctcca gccggatggc cagcgagagc
     6421 cgggtggtgt tgttccggga tcggttcccc cagggagtcg aggagctgat gctctcccgt
     6481 ttcggcagga ccctgttcat accctcgacc cggagccccc tgccttcggc ggatccctac
     6541 cccgaacccc gcatcatcac cgcccagatg gcccaggatt acgagggacc cgaggggatc
     6601 gtggacggct cccggttcga tcatgccatg gtctccaaga tcggccgtgg gatcaacgcc
     6661 gaggtatggt gcccgatcct gtacttccag tacgtcgtcg ggtacgtgta cctggcgaac
     6721 aagaccgacc ggcccgtatc catggatttc tccgtcgtcg actgcgcgtg ggagttttcc
     6781 cgggttctgg ccttctatct gaagaccaac gattacttca agacggccca ggacggcgag
     6841 cccgtctccc acacggcggg catcgtggat ttgtccgcct ccggctgcct cctttccata
     6901 ccccatacgg ccctggcgat gcggatcaag acgggcgccc tgctggacct gcgcctggat
     6961 tatccggagg ggagcctcca agtccggggg aaggtggtcc ggcgcttcga cgacaaggac
     7021 ggagcctact acggagtcgc gttccaggac ttggagtccg aggacctgcg ggtcctcttt
     7081 ccgaagatct acgagatgtc ctacccggat tcccaggagg atgccgggga agcccgcctt
     7141 cacgccgggg gttccgcatg aacgggctga tggccgagct gctgatcgcc ctggaggtcc
     7201 tcaagcggtg ggagatcctg gccgccctcg cggtcttcct gggagtctgg agcgtcttcc
     7261 gatacgtggc gctggttcag cgcaagcccg cgggattccg attccgccgg ccccgaaagc
     7321 cggccgcccc ggccgctccg gctccggctt cggagacgga tgagcctccg gaggaacccg
     7381 aggagtagcc gcggctccgg cgccgggagc cggccgaggt ccgaaccaac gccgccgggc
     7441 ggcaggacgt acccggcctt tccaggcgcc gcgccggggc acctcgatcg cccccgaccc
     7501 cgccaggtag gacagggctt ccgccgcctc ccccgcctcc catccgagga gcgccccggc
     7561 ccaggcacag gggcccgcga ttcccgcgag cagtcgggag gcctccgaca cggtccagcg
     7621 gccggaattg gccgccgcga agcgcaggat ctcccggcgg ccctcgggtt ccggaacggc
     7681 cgaccccgag cagacgtcac agcctgagca tccctccgcc tcggacccca ggagcgcaag
     7741 cagggtttcc ctgcggcacc gggtcgagcc ctcggcgtag tccagcacgg cggccctccg
     7801 gcggcgccgg accggatcgg aatcccggtt cagcaggtcc cggtccgagg ggcggacgat
     7861 gaggaccgcc gtggaaggcc gtccgtcacg gccggccctc ccggattcct gcagatagga
     7921 ttccgcggag ggcggggggt ccgcgtggat gaccgagcgg atgtccggct tgtcgatgcc
     7981 catcccgaag gcgcaggtcg cggtaaggac cccgtcccgg gacgggagga accagtcctc
     8041 cacggccttt cgctcctcct tggtcatgcc cgcatggtag aagcgcacct cccgggtatc
     8101 cagcgcccgg gccaggcgtt ccgcggtgat ctgggttccc gcccgggagg cgcagaagac
     8161 cagcaggggc ttcggcaggg tccgggccag cctttccagg gtatgcgacc gggacagggt
     8221 cggagcgcat cgatagctca ggttcggccg gtccgggttc cccgagacca gccgccacgg
     8281 ttcgtccccg aagagcacct ccgccacacg ggcgaggatg ccctgggagg ccgtcgccgt
     8341 gaaggccgtg acggcgggag gatccagggc ccggaggacg cttcctaatt ccacgtaggc
     8401 ggggcggaag tcgtcgcccc attccgagac gcagtgcgcc tcgtccacga cggcatgggc
     8461 cactcccacc gagcgcagga gggcgaggac ggattccgtc cggaggacct ccgggttggc
     8521 cagggcgatc cgggcccggc ccgaacgcac ggcctcctcc gcggcgcggc gctcctccgg
     8581 ggaccggccg ccctggagta cggcgcaggg cagccccctc tcccggagcc tccgggcctg
     8641 gtccgccatc agggaccgca ggggatagac ggccagggtc agcccgggaa ggaagacggc
     8701 cggaagctgg aagcagaggc tcttgccgta cccggtgggc aggaggacga cttggcgcag
     8761 ccggtccggg ccctccggcg tcgtcccggc ggtgtcgggg acggcgccga gatccccggg
     8821 ggatggcgcc gcccccggga aggcgtccag gatgttggcg accaccaggc gctggtaggg
     8881 atagaggtac cgtaccccga acaaggtttc ggcggcggcc gaaacgggat cctgccgccg
     8941 gatggattcc gatgcgggat cgagcatgcc gggtaaccgc ccgcgatcgg ctgttcgctt
     9001 gcgtccggta cggacggact caggccgtgt ccgccgaggg acagagctcg cggacagcgc
     9061 agcccgggca gtcgggacgg cgggccgtgc agacgtactt accgtgccgg ttgaccgcgt
     9121 gggagaaggc ggtccggtat tccggctcga tcagggcctc gatgcggcgt tccgcggcga
     9181 cgggatcgcg gttctccgtg agcccgagcc tgtggcagac ccggaggaaa tgggtgtccg
     9241 cgatgatccc gggccttccg aagcaggcgc tggccaccag gttggccgtc ttgcgtccca
     9301 cgcccgggag ttccaggagc tcctccatgc gctccggcac ccgtccggaa aacctctcca
     9361 cgaggatcct ggacaggtcg atgaggtgcc gagccttggt ccggtagaag cccagggagt
     9421 ggacgcaatc ctccagctcc gcaaggtccg cctccgctag ccgcccggga tcgggccacc
     9481 tgcggaataa cccgggagtc acccgattga cctggtcgtc cgtcgtctgc gcggagagca
     9541 ccaccgcgac caggagttcg aaacagtcgt cgtaggcgag gaggggctcg gcccgaggcc
     9601 agagggccgt gagggcgagc cggaggctgc gtgcgcgttc cacctcgaag ggggaggttt
     9661 ccatggggaa agtatgccgg gaggctccgg gaagggcaag atctccgacc ggcggtcccg
     9721 cggaccttgc gcggggaagg gttgtatcct atacttacgc aagatcatca gggaggattg
     9781 gatgaaacgg attttgatcg cctcgatcct tatagcgatg gctcttgcgg ccttccccgc
     9841 gttttcccag gaagccaaac cggccgatcc cctggctacc cagatcaaga gcccttccga
     9901 catgtaccct ttccgcttgg acgtggtcaa ggttttccag cacagtctcg gattccgggt
     9961 cgtctaccgg gtcgggcagg cggatttcgc ggacgcctac atccccatcg agtggttcaa
    10021 ggccggcggc aaggccgagt tgatccgttc caacgacccg tcccatccgt acatggtcat
    10081 ctactatcgg gaaggcaaat tcagccatgt ccgactgttc gtcaaggatt tccccaagga
    10141 tccctcctgg gggatcctgg agcccggccc ggacgtggct tccaagttca aggtcgagga
    10201 gatcg
//
LOCUS       DAOTEI010000131        10166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4649_length_10166_cov_6.029671, whole genome shotgun sequence.
ACCESSION   DAOTEI010000131 DAOTEI010000000
VERSION     DAOTEI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4649_length_10166_cov_6.029671"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="P5313_12075"
     CDS             complement(<1..778)
                     /locus_tag="P5313_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRY81147.1"
                     /translation="MNYYDSILKKISDRSVVVGVIGLGYVGLPLAVAFARKGVTVLGF
                     EKSGKKAESVNAGRNYIGDIKDEELAGVVKAGKLSATTDFSRIRECDAAIICVPTPLD
                     KFKKPDMSFIEASCVDIGRNMKAGTFVSLESTTYPTTTEDFMKPILERESGLKEGADF
                     WLCFSPERVDPGNKDYKTDNTPKVVGALGEQARQIALALYGIAIQHLHAVSSPRAAEM
                     VKILENTYRLVNISLINELALLSGKMDINIWEVIEAAKTKP"
     gene            complement(894..1457)
                     /locus_tag="P5313_12080"
     CDS             complement(894..1457)
                     /locus_tag="P5313_12080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRY81148.1"
                     /translation="MSDYFVHESSYVDEGAVIGEGTKVWHFTHVMPGARIGKKCVLGQ
                     NVNVGSKAVLGDRVKVQNNVSLYDDVVLEDDVFCGPSCVFTNVINPRSFVERKHEYLR
                     TLVKKGASIGANATIVCGVTLGEYCFVGAGSVVTKDVPAYALVHGNPARVRGWMCQCG
                     VKLGEDLVCPECGKKYKKAGPGLILEE"
     gene            complement(1586..2701)
                     /locus_tag="P5313_12085"
     CDS             complement(1586..2701)
                     /locus_tag="P5313_12085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY81149.1"
                     /translation="MTVPFYTSTREYAARKDEFDAAVRSVMERGDFILGSEVAEFERE
                     AAAWLGAKYAVGVASGSDALVIAADLLGFRDGAEVLTPTFTFFASTSCVSRLGGKPVL
                     VDLDAETLNMDLRDAERRMSKATKGIIPVHLFLQPTPMQPVMDLARKRGLRVLEDAAE
                     AWGMDCLVDGSWRKAGTVGEIGIYSFFPTKTLGAYGDAGLMVTDDEELYKKIKSYRVH
                     GSSVKYHHDYVGYNSRLDTLQAAILRVKMRTTDAAVAARARHGAHYTERLSRVPGLRL
                     PKILPGGKPVYYVFNIFAPRRDELAAFLKEKGIGTSIYYPIPLHLQKCFQNLGYKKGD
                     FPNAERACGEVLALPIFPELTDDEVDYVCDQIEAFYR"
     gene            complement(2698..3114)
                     /locus_tag="P5313_12090"
     CDS             complement(2698..3114)
                     /locus_tag="P5313_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRY81150.1"
                     /translation="MIKDEVSERIIGCAFQVYNILGSGFLESVYKKALVIELTKAGLT
                     VEEETPITVTYKGQIVGWFNADLIVEKKIILELKAIDVLAKTHEIQLVNYLKATGIPV
                     GLLINFGTTGIQIKRKYKDPVDPDHPGILSKEEESQ"
     gene            complement(3162..4259)
                     /locus_tag="P5313_12095"
     CDS             complement(3162..4259)
                     /locus_tag="P5313_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY81151.1"
                     /translation="MHTLTLALIGCGRISHKHIEAAVKNVDRLRLVACCDPKLDRAEA
                     KAAEYRKAFPDAKVAVYADYREMLAKERPDLCAIATESGYHPRISMDCSAAGAHVVCE
                     KPMALSTADADAMIAAARKAGRTLSVCFQNRFNAPVQRTRTALEAGRFGRMLHGMVQV
                     RWNRNEAYYAEAPWRGTWDLDGGTLMNQCTHGIDLLQWMMGEDAVRVQAATRRFLRPI
                     EAEDFGAAVVEFASGAVGIIEGTADIYPTNLNETLSLFGQTGSVVIGGLAVNKIETWR
                     FADAAVAGDPEDKVVNPAEKDPPSVYGFGHSALYADVLSAIESGREPLVSGEKGRKAL
                     EIILAIYKSQKTGKAVDLPCEFSTVEMKGVF"
     gene            complement(4333..5400)
                     /gene="rfbB"
                     /locus_tag="P5313_12100"
     CDS             complement(4333..5400)
                     /gene="rfbB"
                     /locus_tag="P5313_12100"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRY81152.1"
                     /translation="MRTFQNLLVTGGAGFIGVNFIRYLFRETDFQGRIINLDCLTYAG
                     NPASLSAVLDSSGDRYIFVHGDIRDRAVVDKVIKDYFVDGIVHFAAESHVDRSILGPE
                     AFITTNVIGTFTLLEAARHAWKDREDVLFHHISTDEVFGSLGDEGYFTENTPYDPRSP
                     YSASKAGSDHLVRAYHHTYGLPVTLSNCSNNYGPYQFPEKLIPLMILNMLEDKPLPVY
                     GDGRNIRDWLYVADHAAAIWKIMQSAELGNSYNVGGENEWENIRLLEKLIDIVAMKIG
                     KPAEAIRSKITYVKDRPGHDRRYAIDCSRLKKDLGWNQSVTFDQGLELTVDWYLSNPD
                     WVNSVRSGEYRSWLERNYSKR"
     gene            complement(5403..6281)
                     /gene="rfbD"
                     /locus_tag="P5313_12105"
     CDS             complement(5403..6281)
                     /gene="rfbD"
                     /locus_tag="P5313_12105"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRY81153.1"
                     /translation="MIWVVGDKGMLGQEIGGLLKVEGLKWVGSDREVDFLDPVALREF
                     AKENSPSHIVNCAAYTAVDRAEDEEALALRLNAEGPENLAKLSVDFGARLIHISTDYV
                     FSGDSSVPYTESEPVNPSGAYGRTKAEGERRILAVAPDAIIIRTAWLYGRHGPNFVST
                     MIRLMKERDSLGVVADQRGSPTWARDLAAAILQIVSIAEFPSGIYHYTNEGETTWHEF
                     AVEIQKQGLEMGILNRSCPIRALRTEEYPTRAKRPRYSVLDKSKIRSFGVYVPEWRRS
                     LYSYLSQLTMENERSR"
     gene            complement(6278..6814)
                     /gene="rfbC"
                     /locus_tag="P5313_12110"
     CDS             complement(6278..6814)
                     /gene="rfbC"
                     /locus_tag="P5313_12110"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRY81154.1"
                     /translation="MGIEVKQCPIPGLLEIQPRIFGDSRGYFFESWSARDYEAAGIRT
                     RFVQDNESFSCRGVLRGLHFQKDRPQGKLVRAVRGEIFDVAVDIRPESPNRYRWHSVI
                     LSGDRHNLFYIPPGFAHGFLVLSETAIFSYKCTDFYFPEDEGGLAWDDPKLDIAWPSL
                     GMEYILSEKDRRNPRLPI"
     gene            complement(6805..7683)
                     /gene="rfbA"
                     /locus_tag="P5313_12115"
     CDS             complement(6805..7683)
                     /gene="rfbA"
                     /locus_tag="P5313_12115"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRY81155.1"
                     /translation="MKGLILAGGTATRLFPATRSVSKQMLPVFDKPMIYYPLSTLMLA
                     GIREVLVISTPRDVPHFRDLLGDGSRLGMRFEYAVQDKPRGLADAFIVGERFIGRDQS
                     ALVLGDNVFYGQGFTAMLERAAGRETGATIFGYPVKDPSSFGVVEFDKSGKAISIEEK
                     PAVPKSHYAVPGLYFYDTDVVEIAKSVQPSARGEIEITSVNNEYLGRGDLYVERLGRG
                     FAWLDTGSPATLLEASNFVETVQRRQGLYIACIEEIAFQRGWIGARELVSAAEEMGKT
                     DYSAYLRYLAEETPWA"
     gene            complement(7822..9009)
                     /locus_tag="P5313_12120"
     CDS             complement(7822..9009)
                     /locus_tag="P5313_12120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY81156.1"
                     /translation="MRIAILASESMSGGNNVVFEHAGNLVDRGYKVTMVVDGEVDKKK
                     LSWHRRGSELEWISLELAKQHTYDIAIATFWRTCYKIISIPASRYLYFNQSVESRFFP
                     DEDTLNRSFSELTYCLGFYIVTEASWIKTYIKEHYNQDAFFVPNGVNKEIFKLDGPCY
                     ASRNHGNLRVLVEGPLNIPFKNVQRTIDLCRQAGVDEVWLLTSTVATHVDGVDQLFSR
                     VPIAETPPIYRSCDAIVKLSFIEGMFGPPLEMFHCGGTAVVYNVTGHEEYIRNGHNAL
                     VAEMGDESAVLQHLRRLREDPELLATLKAHALETARDWIDWNSSTDRFEFALQEIVEK
                     FPLNACTVITKTRLVNTLASHASTTTQVNLEASFFKLSFRKFFILTGRFLVRKIKKRF
                     GIY"
     gene            9317..>10166
                     /locus_tag="P5313_12125"
     CDS             9317..>10166
                     /locus_tag="P5313_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HRY81157.1"
                     /translation="MTTNATIAQKAARRKLTLLELASDLDNVSKACKIMGYSRQQFYE
                     IRRNFQTIGSEALLDKVRGPRNPHPSRLSEELEQVLLDYCLEYPTQGSLKVSQQLLLR
                     GIHIGAGAIRGVWQRHNLLSKHQRLLRLEQHARETGMELSEQEIRLLERFDPEFRERH
                     IQADFTGQLVAIDTFMVGNLKGIGKVYLQTVVDCHSRYAWGHLYNSKIPITSVHVLNE
                     RVLPFFEEHGARIHTILSDNGREFCGRPDQHPFELFLQLEDIEHRTTQVRRPQSNGIV
                     ERLHRTL"
BASE COUNT         2278 a         2975 c         2643 g         2270 t
ORIGIN      
        1 agggcttggt cttggccgcc tcgatgacct cccagatgtt gatgtccatc ttgcccgaaa
       61 gcagggccag ttcgttgata agggagatgt tcaccaggcg ataggtgttc tccaggatct
      121 tgaccatctc cgcggcccgg ggtgagctga cggcgtggag gtgctggatc gcgatcccgt
      181 acagggccag tgcgatctgg cgggcctgct cgcccagggc gcccacgacc ttgggggtgt
      241 tgtcggtctt gtagtccttg ttgcccggat ccacccgctc ggggctgaag cagagccaga
      301 agtccgctcc ctccttcaac ccggactccc gctccaagat gggcttcatg aagtcctcgg
      361 tggtagtcgg gtatgtggtg ctctccaggg aaacgaaagt gccggccttc atgttcctgc
      421 cgatgtccac gcaggaggcc tcgatgaagc tcatgtcggg cttcttgaac ttgtccaggg
      481 gcgtgggcac gcagatgatc gcggcgtcgc actcccggat gcgggaaaag tccgtggtcg
      541 ccgagagctt gccggccttc acgaccccgg ccaactcctc gtccttaatg tcgccgatgt
      601 agttcctgcc ggcgttgacg gattccgcct tcttcccgct cttctcgaag cccaggaccg
      661 tcacgccctt gcgcgcgaag gccaccgcca ggggcagacc cacgtagccc aggccgatga
      721 cgccgaccac gacggacctg tcggagatct tcttcagaat agaatcatag tagttcatgc
      781 attctcccaa aattggaaat aagacaggat aacaggattg tcaggatgta caggattaat
      841 tatgggatca ccaaacagtg atcttgttca tcctggaaat cctgttatcc tgtctattct
      901 tctaatatta atccgggtcc tgccttcttg tattttttcc cgcattcggg gcagaccagg
      961 tcttccccca gcttgacgcc gcactggcac atccagcccc ggacccgggc cgggtttccg
     1021 tgcaccaggg cgtaggcggg cacgtccttg gtcaccacgc tgccggcccc cacgaagcag
     1081 tactccccca gggtcacgcc gcacacgatg gtggcgttgg caccgatgga ggcgcctttc
     1141 ttcaccagcg tccgcaggta ctcatgcttg cgctccacga aggaccgggg attgatgacg
     1201 ttggtgaaca cgcaggaggg gccgcagaag acgtcatcct ccaggaccac gtcgtcgtag
     1261 agggagacgt tgttctggac cttcacccga tcccccagga cggccttgct cccgacattc
     1321 acgttctgcc ccaggacaca cttcttcccg atccgcgccc cgggcatcac atgggtgaag
     1381 tgccagacct tggtcccctc cccgatcacc gccccctcgt cgacatagga cgattcatgt
     1441 acaaagtagt cgctcatgag ttctcccgag aaatcagaca ggatgtacag gatgttgaaa
     1501 aggatgaaca agatggaatc atgaggatga gcgaatattg ccctatcctg ttcatccggt
     1561 ctccatcctg ttcatcctgt ccatattagc gataaaacgc ctctatctgg tcgcaaacgt
     1621 agtcgacttc gtcgtccgtg agttccggga agatgggcag agccaggact tccccgcagg
     1681 cccgctctgc gttcgggaag tcgccctttt tgtaccctag attctggaaa catttctgca
     1741 ggtgcagggg gatggggtag tagatcgagg tgccgatccc cttctccttg aggaaggccg
     1801 cgagttcgtc ccggcggggc gcaaagatgt tgaacacgta gtacacgggc ttcccgcccg
     1861 gaaggatctt gggcaggcgg agccccggga ctcgggacag tcgctccgtg tagtgcgccc
     1921 cgtgtcgggc gcgggccgcg acggccgcgt cggtggtccg catcttcacc cgcaagatcg
     1981 ccgcctggag ggtgtccagc cgggagttgt accccacgta gtcgtggtgg tacttcacgg
     2041 agctcccgtg gacccggtag ctcttgattt tcttgtacag ttcctcgtcg tcggtgacca
     2101 tgagccccgc gtccccgtag gcccccaggg tcttggtggg gaagaaggag tagatgccga
     2161 tctcgccgac cgtgcccgcc ttgcgccacg atccgtccac gaggcagtcc atgccccagg
     2221 cctcggcggc gtcctccagg acccgcaggc cgcgcttccg cgccaggtcc atcaccggct
     2281 gcatcggcgt gggctgtagg aagaggtgca cggggatgat gcccttggtg gccttggaca
     2341 tgcgccgctc agcgtcccgg agatccatgt tcagggtctc cgcgtccagg tccaccagca
     2401 ccggcttgcc gcccaggcgc gacacgcagg aagtggaggc gaagaacgtg aacgtggggg
     2461 tgagcacctc ggccccgtcc cggaagccca ggaggtccgc ggcgatcacc agggcgtcgg
     2521 aacccgaggc cacgcccacg gcgtacttcg cgcccagcca ggcggcggcc tcgcgctcga
     2581 actccgcgac ctctgagccc aggatgaagt ccccgcgctc catcacggag cgcacggcgg
     2641 cgtcgaactc gtccttccgc gcggcgtact cgcgggtgga tgtgtagaac ggtacggtca
     2701 ttgggattcc tcttccttgg acaggatacc aggatggtca ggatcgacag gatctttgta
     2761 cttccgtttg atctgaattc cggttgtccc gaaattaatt aaaagcccca cagggatacc
     2821 tgtggccttc agataattca caagctggat ctcatgagtc ttggccagaa catctatcgc
     2881 tttcagttca aggatgatct tcttttcgac tataagatca gcattaaacc atcctacgat
     2941 ttgtccttta taggtgaccg tgatcggtgt ttcttcctct accgtaagac cggcttttgt
     3001 tagttcaatc accaaggctt ttttatagac actttccagg aatccggatc ccagaatatt
     3061 atacacttga aaagcacaac cgatgattcg ctccgatacc tcatccttga tcattcttga
     3121 tcctgtccat cctgtatatt ctgttatcct gtccctcttc ctcagaaaac gcccttcatt
     3181 tccacagtag aaaactcgca cggcaggtcc acggccttgc cggtcttctg gctcttgtag
     3241 atcgccagga tgatctccag cgccttgcgg cccttctccc cggagaccag gggctcccgg
     3301 ccggactcga tcgccgagag gacgtccgcg tacagggccg agtgtccgaa gccgtagacc
     3361 gagggggggt ccttctccgc ggggttcacg accttatcct ccgggtcccc cgcgacggcg
     3421 gcgtccgcga agcgccaggt ctcgatcttg ttcaccgcca ggcccccgat caccacgctc
     3481 ccggtctggc cgaagaggga aagggtctcg ttcaggttcg tggggtagat atccgcggtc
     3541 ccctcgatga tccccaccgc cccggaggcg aactccacca ccgcggcgcc gaagtcctcc
     3601 gcctcgatgg gccggaggaa acggcgggtg gccgcctgga cgcggacggc gtcctcgccc
     3661 atcatccact ggagaaggtc gatgccgtgg gtgcactggt tcatgagggt gcccccgtcc
     3721 aggtcccagg tgccgcgcca gggagcctcg gcgtagtagg cctcgttgcg gttccaccgg
     3781 acctggacca tgccgtggag catgcggccg aagcgcccgg cctccagggc cgtccgggtc
     3841 cgttggaccg gggcgttgaa gcggttctgg aagcagaccg agagggtccg gccagccttc
     3901 cgggccgccg cgatcatggc gtccgcgtcc gcggtggaca gggccatggg cttctcgcag
     3961 accacgtggg cccccgccgc ggagcagtcc atcgagatcc gggggtggta cccggactcc
     4021 gtcgcgatcg cgcagaggtc gggccgctcc ttggcgagca tctcccgata gtccgcgtag
     4081 accgcgacct tggcgtccgg aaaagccttt cggtattccg cggccttggc ctcggcccgg
     4141 tcgagcttgg ggtcgcagca ggcgacgagc cggagacggt ccacgttctt gacggccgcc
     4201 tcgatgtgct tgtggctgat ccggccgcag ccgatcaggg cgagtgtgag agtatgcatg
     4261 ctgaatgacc tttccggcaa gaatttcaac tcatgcttgt gtaattatga atagcaatgc
     4321 atggaaagag ttttaccgct tgctgtaatt ccgctccagc caagaccggt actccccgct
     4381 ccggacggag tttacccaat cgggattcga caggtaccag tcgaccgtca attccagacc
     4441 ttgatcgaag gttacggatt ggttccatcc aaggtccttc ttgagccgtg aacaatcaat
     4501 cgcgtatctc cgatcgtgac ccggcctgtc tttcacatag gttatcttgg agcggatagc
     4561 ttccgcaggt ttgccgatct tcatcgcaac aatgtcgatc aatttttcca gcaggcggat
     4621 gttttcccat tcattttctc cgccaacgtt ataggagttt ccgagttccg cggattgcat
     4681 gatcttccaa atagctgcgg catgatccgc cacatacagc caatcccgga tgttcctgcc
     4741 atccccgtat acgggaagag gcttatcctc aagcatgttc agaatcatca agggtatcag
     4801 cttctcggga aactggtagg gaccgtagtt gttcgagcaa ttcgacaagg tgacaggcaa
     4861 tccgtaggta tgatgatacg ctcgaaccag atgatcgctt cccgccttgg aagccgagta
     4921 cggtgaccgg ggatcatacg gcgtgttttc cgtgaaatac ccctcatctc ccagtgagcc
     4981 gaaaacttca tccgtgctga tgtgatgaaa aaggacatct tcccgatctt tccaggcatg
     5041 ccgggccgct tccagcaggg taaaggtccc aatgacattc gtagttatga atgcttccgg
     5101 gccgagaatg gaacgatcta cgtggctttc cgcggcaaaa tggactattc catcgacgaa
     5161 atagtctttt atcaccttat cgactaccgc tcgatcccgg atgtccccat gtacgaaaat
     5221 gtaacggtct cccgatgaat caagtacagc tgatagactg gcaggattac ccgcatacgt
     5281 caagcaatcc aggttaatga tccttccttg aaaatccgtt tcccggaaaa ggtatcgtat
     5341 gaaatttacc ccgataaaac ctgcgccacc ggttaccaac agattctgga atgtacgcat
     5401 gtttaccgac tcctttcatt ttccatggtt aattggctca agtacgaata aagacttctc
     5461 ctccactcgg gaacgtatac tccgaaagag cgaatcttgc ttttatcgag tactgagtat
     5521 ctaggccgtt ttgcgcgtgt cgggtactct tccgtgcgca aagctcgaat cggacagcta
     5581 cggtttagga ttcccatctc caatccttgc ttctggatct ccacggcgaa ttcatgccag
     5641 gtggtctccc cttcattcgt gtaatggtaa ataccggacg ggaattccgc aattgatacg
     5701 atttggagaa tcgcagccgc caaatcccgg gcccaggtgg gagaacctct ttgatcagcc
     5761 acaacaccca gggaatcccg ttccttcatt aaacgaatca tggtcgatac aaaattcggg
     5821 ccgtggcgac catagagcca agcggttcga atgattatgg catccggcgc gacagcgaga
     5881 atccgtcgct cgccctccgc cttggtacgc ccgtacgcgc ccgaggggtt gacaggttcc
     5941 gattccgtat agggaacgga ggagtctccc gaaaaaacat aatccgtgga tatgtgaatg
     6001 agtctcgccc cgaaatccac cgaaagcttc gccaggtttt ccggtccttc ggcattcagc
     6061 ctgagcgcta gagcctcctc gtcctcggca cgatccaccg ccgtatacgc ggcacaattg
     6121 acgatatggg agggagagtt ctctttcgca aactcccgga gcgcgacagg atccagaaaa
     6181 tccacttccc ggtcgctccc gacccacttc aacccctcaa ccttcaggag tccgccaatc
     6241 tcctgtccca acattccctt gtcgccgact acccagatca tatcggcagc ctcgggtttc
     6301 tacgatcctt ctcggacaga atgtactcca tgcccaaaga aggccaggct atatccagtt
     6361 ttgggtcatc ccatgccaaa ccgccctcat cctccgggaa gtagaagtcc gtgcacttgt
     6421 aggaaaatat cgccgtctcg gaaagcacta ggaaaccatg ggcgaagccg ggtgggatat
     6481 aaaatagatt gtgccgatct cccgacagga tcaccgaatg ccacctatac ctgttcggag
     6541 attccggccg gatatccacg gccacatcaa agatttcccc gcgtacggcg cgaacaagtt
     6601 tcccctgggg acgatctttt tggaaatgga gaccccgaag gaccccacga caagagaagg
     6661 actcgttgtc ctgcacaaac cgagttcgta ttcccgccgc ctcgtaatcc cgcgccgacc
     6721 aggattcgaa gaagtacccc cttgaatcgc cgaagattcg gggctgaatt tccagcaagc
     6781 ccgggatcgg gcactgcttg acttctatgc ccatggggtt tcctcggcca aatagcggag
     6841 gtaggccgaa taatccgtct ttcccatttc ttccgcggcg gacacgagct cccgggcgcc
     6901 tatccagcct cgctgaaagg ctatttcctc gatgcatgca atgtagagcc cctgacgacg
     6961 ctggacggtc tccacgaagt tggaggcttc gaggagagtg gcaggactcc cggtatcgag
     7021 ccatgcgaag cctcgaccca gccgctccac atacagatct cctcgcccaa ggtattcatt
     7081 gttaacgctc gtaatctcga tttctccccg ggcggatggc tggacggatt tggcgatttc
     7141 gaccacgtcg gtatcgtaaa aatacaggcc cgggaccgcg tagtgggatt tgggcactgc
     7201 gggtttctct tcgatcgata tcgccttacc ggatttgtcg aactccacga caccgaaaga
     7261 cgaagggtcc tttacgggat acccgaagat ggtagctccg gtctcacggc cggcggctct
     7321 ctcgagcatg gccgtaaacc cctggccgta aaaaacattg tctcccaaca ccaaggcact
     7381 ctggtcccga ccgatgaacc gctcccccac gataaacgcg tccgccaggc ccctcggctt
     7441 atcctggacc gcgtattcaa agcgcatccc cagacgggaa ccgtccccca gaaggtcccg
     7501 gaagtgggga acatcccggg gggtggaaat caccaggact tcccggattc ccgccagcat
     7561 cagcgtggac aggggatagt agatcatcgg cttgtcgaag accggcagca tttgcttgga
     7621 aaccgacctt gtcgccggga acaagcgcgt tgcagtccca cccgcgagaa tgagaccttt
     7681 catcttgctc ctccaataga gatttggaag atttctttgt atacaaagat ttgattacaa
     7741 attgatacga gaataaaagt actgagtaaa ggagaaatga ctcattcctc ttagtttttg
     7801 gtaataatag taagtggccg cttagtatat gccaaatctc ttctttatct ttctcacaag
     7861 aaaacgtcct gtcagaataa aaaactttct aaaagataat ttaaaaaatg aagcttctaa
     7921 gtttacctga gtggtcgtag acgcatgaga tgcaagagta ttaactagtc ttgttttggt
     7981 aattacagta caagcgttta aaggaaattt ttctactatt tcttgaagag caaactcaaa
     8041 tcgatcggtt gaggaattcc aatctatcca atcacgagca gtttccaaag catgagcctt
     8101 taatgtagca agcaattccg gatcttctct caatcgcctt agatgttgaa gaacagctga
     8161 ttcatcgccc atttcggcca cgagagcatt atgcccgttg cgtatatact cctcatgtcc
     8221 tgtgacatta taaacaactg ctgttcctcc acagtgaaac atttctagtg gtggaccaaa
     8281 cataccttca ataaaactaa gtttgacaat cgcgtcacat gaccggtata tcggaggcgt
     8341 ctcagcaata ggtaccctgg agaataactg gtctaccccg tcaacatgtg tggcaactgt
     8401 gctcgttaga agccatactt catcgacacc ggcctgacga catagatcta tggtgcgttg
     8461 aacattttta aatggaatat ttaaaggtcc ttcaactagg accctaagat tgccatgatt
     8521 tctagaagca taacaggggc cgtctaactt gaatatttcc ttattaacgc catttggaac
     8581 aaaaaaagca tcttgattgt aatgttcttt aatataagtt tttatccaac tcgcctcagt
     8641 aactatataa aaacccaaac aataagttag ctctgaaaaa cttcgattca aagtatcttc
     8701 atcggggaaa aaccgcgact caacagactg attaaaatat aaatatcggc tagcaggaat
     8761 actaataatt ttgtagcatg tacgccaaaa cgtcgcaatt gcaatgtcat aagtgtgttg
     8821 ctttgctagc tcaagcgaaa tccattccaa ctcagaacca cgccgatgcc aagaaagttt
     8881 ttttttgtcg acttccccat ctacaaccat tgtcacttta tagccgcggt cgacaagatt
     8941 cccagcatgc tcgaaaacta cgttgtttcc accgctcatt gattcagaag ctagaattgc
     9001 aattcgcaag gctataatca tattaaacca accaaataat tgttttcgta aaactgacgt
     9061 tataaaggtg tgacttgatt tgagcggaaa atttcatgaa aggacatgaa aactggcggt
     9121 cctcccggtc ccagatagta ggctggtttt gcctaaaaac tcttcaccat agaagatcgg
     9181 aacaaccagc aacactatta tagcacagaa tgttttgagc gagaaattac cttacaggaa
     9241 atgaaaaatg gcggtcaccc gcccccggat ggtagaatgg ttttaccgct aaaacagttc
     9301 accaagggag aacggaatga ccaccaacgc cactatagcg cagaaagctg cccgcaggaa
     9361 gctcacgctc ctggaactcg cttccgacct cgacaacgtc tccaaagcct gcaagatcat
     9421 ggggtattct cgccaacagt tctacgagat ccgcaggaac ttccaaacca ttggctccga
     9481 ggctctcctg gacaaagtcc gcggtccccg caacccccac ccaagccgtc tatctgaaga
     9541 gctggaacaa gtccttctcg actactgcct tgagtacccg acccagggaa gcctcaaggt
     9601 cagccaacag ctccttctcc ggggaatcca catcggagcg ggagccatcc gcggcgtctg
     9661 gcagaggcac aacctgttat ccaaacacca gagattgctc cgccttgaac agcacgccag
     9721 ggaaaccggt atggagcttt cggaacagga gatccgcctc cttgaacggt tcgatccgga
     9781 gttccgggaa cgtcatatcc aggctgactt tacaggtcag cttgtcgcga tcgacacctt
     9841 catggtcggg aacctcaaag gcatcgggaa ggtctatctg caaaccgtcg tcgattgcca
     9901 ctcacggtac gcctgggggc acctgtacaa ctctaagatc cccatcacgt ctgtccacgt
     9961 cctcaacgaa cgcgtccttc ccttcttcga ggaacacggg gcgcggatcc acaccatcct
    10021 cagcgacaac ggccgggagt tctgcggacg tccggatcag catcccttcg agctattcct
    10081 ccagctggaa gacatcgaac atcggaccac gcaagtccgc aggcctcaga gcaacggcat
    10141 cgtcgagcgt cttcaccgca ccctgc
//
LOCUS       DAOTEI010000132         9978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4772_length_9978_cov_5.071551, whole genome shotgun sequence.
ACCESSION   DAOTEI010000132 DAOTEI010000000
VERSION     DAOTEI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4772_length_9978_cov_5.071551"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2218
                     /locus_tag="P5313_12130"
     CDS             <1..2218
                     /locus_tag="P5313_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRY81158.1"
                     /translation="TDGAGRPSGAPETIRSLFKTYIRGLTEELALSKDFLRSVFTDNA
                     GVVQFRPGWALCVKAETHNSPSALDPYGGAITGIVGVNRDILGTGMGAKPIFNTDVLC
                     FAPPDTPEGEVPEGLLHPREVLEGVHKGIVDGGNQSGIPTVAGTFLFDESFLGKPLVF
                     CGTGGILPETVNGKPAWEKTVSPGDRAVMLGGRIGKDGIHGATFSSLALDEGSPASAV
                     QIGDPIIQRRMTDFLLEARDRGLYAGITDNGAGGLSSSLGEMAQSSGGVRIDLDACPL
                     KYPGLSPWEILVSESQERMSLAVPPDRIDDFLALAARRGVEATVVGEFTDSGNVEIRS
                     GGRTAGLLSLDFLHDGLPPLRLSARWSPVSLPSDPLPAREDWTETLYRILAEPNSASK
                     EPWVREYDHEVQGRSVEKPFTGVHRDAPSDGAVLKPRYDDGAGITVTHGICPRLSDYD
                     AGLMAACAVDEAYRAHVALGGDPDRAAALDNFCWPDPLPSPGNPDAERKLAQLVRACR
                     GLADACRAYGLPLVSGKDSMKNDAVAGGRRISVRPTLLVTLTGFVGDVRKVPSTDFPG
                     PGLLVYLLGRTEGALGLSLLERVEGLRFGDPPGIDTAAAVPLYRALARGISGRLVLSC
                     HDLSDGGLAAAAAESCLGGRVGADLVLDALPGAEAAEPVRILFSECPSRLLVSVRAAD
                     RERFERSFSGLPHRLLGTTTAEPRLRFRHAGREVLSAALDGLESAYKTPIAGRRYP"
     gene            2215..2952
                     /locus_tag="P5313_12135"
     CDS             2215..2952
                     /locus_tag="P5313_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRY81159.1"
                     /translation="MSAPVLIACGFGINSDEELAEAFRQAGADPSRIHLTDLLAEPGM
                     LARFRILAFPGGFSFGDHLGSGMVLANLLRRRLRPEIEAFLADGGLAIGICNGFQVLA
                     KTGILTNLRGGWEREITLVHNEKPGYVDDWVRVRVEPECPCVWTKGLSTLDLPVRHGE
                     GRFTARDPGTLARLESERLVALRYEGRNPNGSANGIAGVCDRTGRVLGLMPHPEAFLI
                     PDNHPDRRRRPPRETGLEIFRNAVARS"
     gene            3011..4060
                     /gene="dgcA"
                     /locus_tag="P5313_12140"
     CDS             3011..4060
                     /gene="dgcA"
                     /locus_tag="P5313_12140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HRY81160.1"
                     /translation="MTKDMKEESTAAIEEYERQIFDLRQLLEISKSLNSTLDYTILID
                     SILFTILGQMKVLKAGLFAKKNLDSPSFSLHRNHQGFDLRRDVEYSIPEDHPLVAYFT
                     RAYGCLTLDEIRRSLGSLAGLEGLAALEPSLVIPLKAKGVINGILVIGERIEGDEFSA
                     TEREYVINIANLAAIAISNAFLFEMTTTDMMTKLKMKHYFYTVLLERMDRARTDRRPL
                     SVLMLDIDHFKRFNDTYGHSCGDLVLKQTAVVLQESVRPGDLAARYGGEEFCLLLPDT
                     DGPHAACIAERIRKGVEANRIAYEGKTLGVTVSIGAAGYDPARDISAKTLLDRADKAL
                     YLSKQEGRNRTTLSP"
     gene            4066..4404
                     /locus_tag="P5313_12145"
     CDS             4066..4404
                     /locus_tag="P5313_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY81161.1"
                     /translation="MAVLRYRAPGSPLETEVTANLVDSILTSLLKAGRPIRHDCGGKA
                     LCGTCRVRVLEGAAALSPISDRERSRLSAVGAAPDERLACQAHAARDAKIEAVLPLEG
                     PGPETKGVTG"
     gene            4410..4769
                     /locus_tag="P5313_12150"
     CDS             4410..4769
                     /locus_tag="P5313_12150"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRY81162.1"
                     /translation="MEFDEEEARALARSMDRALYYVYLLKKGSRWSPDSSPEIDALQE
                     AHLANLRRLGEEGKLVLNGPLLDSFQLSGELRGIGVLKAKSLAQAREWIGTDPMVQAG
                     RLVFEVHAWMVPKGILP"
     gene            4904..6547
                     /locus_tag="P5313_12155"
     CDS             4904..6547
                     /locus_tag="P5313_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRY81163.1"
                     /translation="MQWWNDAVFYQIYPRSFQDSNGDGVGDLAGIESRLDYLAALGVD
                     ALWISPFFKSPMKDFGYDVSDYVSVDPLFGDMDGFKRLLSAAHRRNLRVVVDLVLNHT
                     SDEHPWFREAQESRDSPKHDWYIWKPIPERGPRRPPNNWISLFELRSAWYENPASREW
                     YLATFTRNQPEVNWRNPELREAMYGVVRAWLDLGVDGFRLDVATGYFKDELFRTNPFK
                     PRLVPDLLQEHLYDRNRPEVHGIFRDLRRIADSYGGRVLIAEPHGQDRALAASCHGSA
                     GDELHMAFNFEFLTRPWGAAAFRDSARRWYEALPPGAWPNFTLSNHDQPRHIHRYRRG
                     AETEARARVAAAMLLTLRGTPFLYYGEEIGMGCVRLPRGALRDPLGIRTWPLSGFGRD
                     PERTPMQWDASAGAGFTTGTPWLPVNPDHPVRNVAAQEGNPDSLLAFYKKLLALRRKT
                     PALRSGDLEFLGEDPDVLAYRRTPAEAAETRKAGSDSRGPAVLVVLNFAGGRKTFALP
                     EGGRVLAGTGRAVGTRVEGGTVGLAPCEVLILETEGRSR"
     gene            complement(6544..7554)
                     /gene="holA"
                     /locus_tag="P5313_12160"
     CDS             complement(6544..7554)
                     /gene="holA"
                     /locus_tag="P5313_12160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRY81164.1"
                     /translation="MPPPSVEILAGPETGRRNDRIAQIRRDCAEAWGSPPEEHRLYAF
                     ETGVQDLLGLLRNGSLFSDGKLVLLFGAEAVKGKSDLAALAQYFRAPAEKTALILVTE
                     GYGIEKYLEDAAGKDGKKIFWELGADEKDRWVRDYFRREGLEIEPEAVEAILELVENN
                     TEALKTECSRLALFFPRGGKVTADAVEAYIAHNREEDAFSLFDRLASGDLERSLETLG
                     AILVSREGTGIGLLGGLLWSFRRLRALEEDLAEGAAWEQAVRLQRITSRKLQAVYDAA
                     RRLWPRSVCESLIAFGVETDAQLRALGSPYERLLLELFVYACAGKKGPVPLAVPAGER
                     GP"
     gene            complement(7554..8153)
                     /gene="lexA"
                     /locus_tag="P5313_12165"
     CDS             complement(7554..8153)
                     /gene="lexA"
                     /locus_tag="P5313_12165"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRY81165.1"
                     /translation="MKDLTDRQKEVLAYISSFSEEHSYPPTIREIAERFGISVKGAYD
                     HVKALERKGTLRLGENRSRSIEVLAREKPGARYAEVPLLGTVAAGRPILSEENLDGTI
                     RIPAEMLRSRPGFALRVRGDSMRDAGILDGDVAVIEERPVAENGEIVVAMIDDAVTLK
                     RFHRETGRVRLSAENPSYAPIYTQDARILGRLRGILRTY"
     gene            complement(8150..9517)
                     /locus_tag="P5313_12170"
     CDS             complement(8150..9517)
                     /locus_tag="P5313_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY81166.1"
                     /translation="MNTILVIDDEPAIRAAVRDILQDEKYRVLLAEDGIVGLDILETE
                     RADLVILDVRLPRMGGMDVLREIRKRKPELEVLVVSGHGTIDMAVQAVRLGAFDFIEK
                     PLSIDRLLTAVRNGLSMAALRAENRRLRSAVLGEDEIIGDSAAIREVRDLADQAAKSD
                     ARVLITGENGTGKELVARRIHGASGRARGPFVAVNCAAIPDTLIESELFGHEKGAFTD
                     AVSRRIGKFEAADGGTLFLDEIADMSLTAQAKVLRAIQDMRFERIGGQESLSADVRVI
                     AATNKDLDAQIAAGRFREDLYFRLAVVLVRVPPLRSRREDIPGLCRFFLSQKSGGNGQ
                     ARELSAGALRFLSGHDWPGNIRELRNLMERVSVLSDEAEISEATVSRLLGSGERVTAG
                     PCLVPGEYSGLKLLAAKELFERNYLVQKLRESEYTITKAAQAAGIYPSALHAKMKKYG
                     IESGE"
     gene            complement(9531..>9978)
                     /locus_tag="P5313_12175"
     CDS             complement(9531..>9978)
                     /locus_tag="P5313_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY81167.1"
                     /translation="DWTNLRTLVEESCSPYRASFPEARFDAENVDPDLVLRVDRTRMR
                     QALENLISNALDAMDGKGSVTFRSDLVKAAESRYCRLQIRDTGRGIPPENRDRIFVPY
                     FTTKRAGTGLGLSITERIVQDHGGRVRFESEPGAGTVFFLDLPFDS"
BASE COUNT         1527 a         3498 c         3378 g         1575 t
ORIGIN      
        1 gaccgacggg gcgggccgtc cctcgggtgc tcccgagacg atccgctccc tcttcaagac
       61 ctacatccgg ggcctcacgg aggagctggc cctctccaag gacttcctgc gctcggtatt
      121 cacggacaac gcgggggtcg tccagttccg ccccggctgg gccctctgcg tcaaggcgga
      181 aacccacaac agcccctccg ccctggatcc ctacggcggg gcgatcacgg ggatcgtggg
      241 ggtcaaccgg gacatactgg gcacgggcat gggggccaag ccgatcttca acaccgacgt
      301 cctctgtttc gcgccgccgg acaccccgga gggcgaggtc cccgaggggc tcctgcatcc
      361 ccgggaggtc ctggaggggg tgcacaaagg gatcgtggac gggggcaacc agtccggaat
      421 ccccacggtc gcggggacct tcctcttcga cgagagcttc ctgggcaagc ccctggtttt
      481 ctgcggcacg ggcggcatcc ttccggagac ggtgaacgga aagcccgcct gggagaagac
      541 cgtgagcccc ggggaccggg cggtcatgct ggggggccgg atcggcaagg acgggataca
      601 cggggccacg ttttccagtc tggccctgga cgaggggagc cccgcctcgg cggtccagat
      661 cggggatccc atcatccagc ggcgcatgac ggacttcctc ctggaagccc gggaccgggg
      721 gctctacgcg gggatcacgg acaacggggc cggaggcctg tcctccagcc tgggggagat
      781 ggcccagtcc tccggggggg tccggatcga cctggacgcc tgtcccctca aatatcccgg
      841 gctttccccc tgggagatcc tggtctccga aagccaggag cggatgagcc tggcggtccc
      901 gccggaccgg atcgacgact tcctggccct ggcggcccgg cggggggtcg aggcgacggt
      961 ggtcggggag ttcacggact cggggaacgt cgagatccgg tccgggggcc gcaccgcggg
     1021 cctcctgtcc ctggacttcc tgcacgacgg gcttccgccc ctgcgccttt ccgcccgctg
     1081 gtccccggtg tccctcccct cggaccccct ccccgcccgg gaggactgga cggagaccct
     1141 gtaccggatc ctggcggagc cgaactccgc ctccaaggag ccctgggtcc gggaatacga
     1201 ccacgaggtc cagggccgct ccgtcgagaa gcccttcacg ggagtccatc gggacgcccc
     1261 ctcggacggg gcggtcttga agccccggta cgacgacggg gccgggatca cggtcaccca
     1321 cgggatctgc ccccgccttt cggactacga cgcgggcctc atggccgcct gcgcggtgga
     1381 cgaagcctac cgggcccacg tcgccctggg gggggacccg gaccgggccg cggccctgga
     1441 caacttctgc tggcccgacc ccctgccctc cccggggaac cccgacgcgg agcgcaagct
     1501 ggcccagctg gtccgggcct gccggggact ggcggatgcc tgcagggcct acggtctgcc
     1561 cctggtctcg ggcaaggatt ccatgaagaa cgacgcggtc gccggcggcc gccgcatctc
     1621 cgtccggcct accctcttgg tcaccctgac gggcttcgtg ggggacgtac gaaaggttcc
     1681 gtccacggac ttcccgggcc cgggtctcct ggtctacctc ctgggccgta cggagggagc
     1741 cctggggctc tcgctcctcg aacgggtgga aggcctgcgg ttcggggatc ccccgggaat
     1801 cgataccgcc gcggcggtcc ccctctaccg ggccctggcc cgggggatct cgggccgcct
     1861 ggtcctgtcc tgccacgacc tgtccgacgg gggcctggcc gccgcggcgg cggagtcctg
     1921 cctgggcgga cgggtcgggg cggacctggt cctggacgcc ctgcccggag ccgaggccgc
     1981 ggagccggtc cggatcctct tctcggaatg cccctcccgg ctccttgtgt ccgtgcgggc
     2041 agcggaccgg gagcgcttcg agcggagctt ctccgggctt ccccaccgtc tcctggggac
     2101 cacgacggcg gaaccccggc tgcgcttccg gcacgcgggc cgggaggtcc tgtccgcggc
     2161 cctggacggg ctcgagtcgg cctacaagac gccgatcgcc ggaaggaggt acccgtgagc
     2221 gcgccggtcc tgatcgcctg cggcttcggc atcaactccg acgaggagct cgccgaggcc
     2281 ttccggcagg ccggggcgga cccctcccgg atccacctga cggacctcct ggcggagccg
     2341 gggatgttgg cccggttccg catcctggcg ttcccggggg gcttctcctt cggggaccat
     2401 ctgggttccg gcatggtcct ggccaacctg ctccgtcggc ggctccgccc ggagatcgag
     2461 gccttcctgg cggacggagg actggccatc ggaatctgca acgggttcca ggtcctggcc
     2521 aagacgggga tcctcacgaa ccttcggggc ggctgggaaa gggagatcac cctggtccac
     2581 aacgagaagc ccggctacgt ggacgactgg gtgcgggtcc gggtcgagcc ggaatgcccc
     2641 tgcgtctgga cgaagggcct ctcgaccctg gacctgcccg tgcgccacgg ggagggccgg
     2701 ttcacggccc gggacccggg caccctggcc cggctggaat cggagcgact cgtggccctc
     2761 cgctacgagg ggcggaaccc gaacggctcg gcgaacggga tcgcgggggt ctgcgaccgg
     2821 accggccggg tcctgggcct catgccccac ccggaggcct tcctgatccc ggacaaccac
     2881 ccggatcggc ggaggcgccc accccgggag acggggctgg agatcttccg gaacgccgtg
     2941 gcacggtcgt agattttcga ggcacaagcg cctatactga ggcgagaacg atacttagga
     3001 tcgtaccggg gtgacgaagg acatgaagga agaatcgacc gcggccatcg aggagtacga
     3061 acgccagatc ttcgacctcc gccagctcct ggagatctcg aaaagcctca actcgaccct
     3121 ggactacacc atcctcatcg attccatcct gttcaccatc ctgggacaga tgaaggtcct
     3181 caaggcgggg ctcttcgcca agaagaacct ggattccccc tccttcagcc tccaccggaa
     3241 ccaccagggc ttcgacctcc ggagggacgt ggagtactcc atccccgagg accatcccct
     3301 ggtggcttat ttcacccggg cctacggctg cctgaccctg gacgagatcc gccgatccct
     3361 gggatccctg gccggactgg aggggctggc ggccctggaa ccaagcctgg tgatccccct
     3421 caaggccaag ggggtcatca acgggatcct ggtcatcggg gaacggatcg agggggacga
     3481 gttttccgcc accgagcggg agtacgttat caacatcgcc aacctggcgg ccatcgcgat
     3541 cagcaacgcc ttcctcttcg agatgaccac cacggacatg atgaccaagc tcaagatgaa
     3601 gcactacttc tacaccgtgc tcctggagcg gatggaccgg gcccggacgg accggaggcc
     3661 cctgtccgtg ctcatgctgg acatcgacca cttcaagcgc ttcaacgaca cctacgggca
     3721 ttcctgcggg gaccttgtgc tcaagcagac cgcggtggtc ctccaggaga gcgtccgccc
     3781 gggggatctg gccgcccggt acgggggcga ggagttctgc ctgctcctgc cggacaccga
     3841 cggcccccat gccgcctgca tcgcggaacg gatccgcaag ggcgtggaag ccaaccggat
     3901 cgcctacgag ggcaagactc tcggggtgac cgtttccatc ggggcggccg gatacgaccc
     3961 ggcccgcgac atctccgcca agaccctcct ggaccgggcc gacaaggccc tatacctgtc
     4021 caagcaggag ggtcgcaacc ggacgaccct ctccccctga gcgccgtggc cgtcctccgg
     4081 taccgcgccc ccgggagtcc gctcgaaacc gaggtcactg cgaacctcgt cgattccatc
     4141 ctgacctccc tgctcaaggc gggccgtccg atccgccacg actgcggcgg caaggccctc
     4201 tgcgggacct gcagggtccg tgtcctggag ggcgccgccg ccctgagtcc gatctccgac
     4261 cgggagcggt cccgcctgtc cgccgtcggc gcggccccgg acgaacgcct ggcctgccag
     4321 gcccacgccg cccgggacgc aaagatcgag gcggtcctgc ccctggaggg cccgggcccc
     4381 gaaaccaagg gcgtgacggg atgaacagga tggaattcga cgaagaagaa gcccgagcgc
     4441 tggctcggtc catggacagg gcgctttact acgtatactt gctcaagaag ggctcccgat
     4501 ggtcgcccga ctcgagcccc gagatcgacg ctctgcagga agcgcatctg gccaacctcc
     4561 gcaggctggg tgaggagggc aaactggtgc tgaatggccc cctcctcgat tccttccagc
     4621 taagcggcga gctgcgcggc atcggagtcc tcaaggcgaa gtcccttgcc caggcgcgtg
     4681 aatggatcgg caccgatccc atggtgcagg cgggccggct ggtcttcgaa gtccacgcct
     4741 ggatggttcc gaagggcatc ctgccctgac cgcctcatcc gtcgggccgt ccaggatcct
     4801 caccatccca cccctcgtat cgatcttttc tccgtgccct ccgtggtgat tcctgtataa
     4861 ttgcattctc attctgcggg tttcatcgaa cgagggggaa cgtgtgcagt ggtggaacga
     4921 cgccgttttc tatcaaatct acccccggtc cttccaggac tcgaacgggg acggggtcgg
     4981 ggacctggcg ggcatcgagt cccggctgga ctacctggcc gccctggggg tggacgccct
     5041 gtggatctcc cccttcttca agtcccccat gaaggatttc ggctacgacg tgtcggacta
     5101 cgtctccgtg gaccccctct tcggggacat ggatggcttc aagcgcctcc tgtcggcggc
     5161 ccaccgccgg aacctccggg ttgtggtgga cctggtcctg aaccacacct ccgacgagca
     5221 cccctggttc cgggaagccc aggagtctcg ggattccccg aagcacgact ggtacatctg
     5281 gaagcccatc ccggagcgcg gcccccggcg gcccccgaac aactggatca gcctcttcga
     5341 gctccgttcc gcctggtacg agaatccggc ctcccgggag tggtacctgg ccaccttcac
     5401 ccggaaccag ccggaggtga actggcggaa cccggagctt cgcgaagcga tgtacggggt
     5461 ggtgagggcc tggctggacc tgggggtgga cgggttccgc ctggacgtgg ccacggggta
     5521 cttcaaggac gagctgttcc gcacgaatcc cttcaagccc cgcctggttc cggacctcct
     5581 ccaggaacat ctttacgacc ggaaccggcc ggaggtccac gggatcttcc gggacctccg
     5641 aaggatcgcg gactcctacg gcggccgggt cctgatcgcg gagccccacg gccaggaccg
     5701 ggccctggcc gcctcctgcc acgggtctgc gggggacgag ctccacatgg ccttcaactt
     5761 cgagttcctg acccggccct ggggggccgc ggctttccgg gactcggccc ggcgctggta
     5821 cgaggcactt ccgcccgggg cctggcccaa cttcaccctg agcaaccacg accaaccgcg
     5881 ccatatccac cgctaccgtc ggggcgcgga gacggaggcc cgggcccggg tcgccgcggc
     5941 gatgctgttg accctgcggg gcactccctt cctctactac ggcgaggaga tcggcatggg
     6001 ctgcgtccgc ctgccccggg gcgccctccg ggatcccctg ggcatccgga cctggcccct
     6061 gtccggcttc ggccgggacc ccgagcgcac ccccatgcag tgggacgcct cggcgggggc
     6121 cggattcacg accggaaccc cctggctccc ggtgaacccc gaccatccgg tacggaacgt
     6181 cgcggcccag gagggaaatc cggactctct cctggccttc tacaaaaaac tcctggctct
     6241 gcgtcggaag acgccggccc tgcgttccgg ggacctcgag ttcctggggg aggatccgga
     6301 cgtcctggcg taccggagga ccccggccga ggcggcggag acgcggaagg ccggctcgga
     6361 ctcccgcgga ccggccgtcc tggtggtcct caacttcgcg ggcggccgga agactttcgc
     6421 cctgcccgaa gggggccggg tcctggcggg cacgggaagg gccgtgggca cccgggtgga
     6481 aggggggacg gtcgggctcg cgccctgcga ggtcctgatc ctggaaacgg agggccggag
     6541 ccgttagggc ccgcgctccc cggccgggac cgcgagggga accggcccct tcttaccggc
     6601 gcaggcgtag acgaagagct ccagcaatag acgctcatag ggggagccga gggcgcggag
     6661 ctgggcgtcc gtctccaccc cgaaggcgat gagggactcg cagacggacc ggggccagag
     6721 ccggcgggcg gcgtcgtaga ctgcctggag cttccggctg gtgatccgct gaaggcgcac
     6781 cgcctgctcc caggccgcgc cctcggccag gtcctcctcc agggcccgga ggcgccggaa
     6841 gctccacagg agtccgccca gaaggccgat gcccgtgccc tcccggctga ccaggatggc
     6901 ccccagggtc tccagggacc gctccaggtc ccccgaggcc agccggtcga agaggctgaa
     6961 ggcgtcctcc tcccggttgt gggcgatgta ggcctccacg gcatcggcgg tgaccttgcc
     7021 tccgcgggga aagaagaggg ccaggcggga acattccgtc ttcagggcct cggtgttgtt
     7081 ctccaccaac tccaggatcg cctccaccgc ctcaggctcg atctccagac cctcccgccg
     7141 aaagtagtcc cgcacccagc ggtccttctc gtccgcgccg agctcccaga agatcttctt
     7201 gccgtccttg cccgccgcgt cctccaggta cttctcgatc ccgtagccct cggtgaccag
     7261 gatcaaggcc gtcttctccg cgggagcccg gaagtactgg gccagggcgg ccaggtcgga
     7321 cttgcccttg acggcttcgg ccccgaacag gaggaccagc tttccgtcgg agaagaggga
     7381 tccgttgcgc aggaggccga ggaggtcctg cacccccgtc tcgaaggcgt acagccggtg
     7441 ctcctccggg ggggatcccc aggcctcggc gcagtcccgg cggatctggg cgatgcggtc
     7501 gttccggcgc cccgtttcgg ggccggcgag gatctccacg gaagggggag gcatcagtag
     7561 gtccggagta tgccccggag gcgccccagg atccgggcgt cctgggtgta gatgggagcg
     7621 taggagggat tctcggcgga taggcggacc ctgccggtct cccggtggaa gcgcttgagg
     7681 gtgacggcgt cgtcgatcat ggccaccacg atctcgccgt tctccgccac cggacgctcc
     7741 tcgatgaccg ccacgtcccc gtccaggatc cccgcgtccc gcatggagtc cccccgcacc
     7801 cgcagggcga agccgggccg ggatcggagc atctccgcgg ggatgcggat cgtgccgtcc
     7861 aggttctcct ccgagaggat gggccttccc gcggccacgg tccccaggag ggggacctcc
     7921 gcgtagcggg ctcccggctt ttcccgagcc aggacctcga tggatcggga acggttctcc
     7981 cccagccgga gggtgccctt ccgctccagg gccttcacgt ggtcgtaggc gcccttgacg
     8041 gatatgccga aacgctccgc gatctcccgg atcgtgggag gatacgagtg ttcctcggag
     8101 aaggaggaga tataggccag cacttccttc tggcggtccg tcagatcctt cattccccgc
     8161 tctctatccc gtatttcttc atcttggcgt gcagggcgct ggggtagatc cccgcggcct
     8221 gggcggcctt ggtgatagta tactcgcttt cccgcagttt ttgtacaaga tagttccgct
     8281 cgaacagttc cttggccgcc aggagcttga ggcccgaata ctccccgggc accaggcaag
     8341 ggccggcggt cacccgttcc ccggaaccca ggaggcggct caccgtcgcc tcggagatct
     8401 cggcctcgtc ggagaggacg gaaacccgct ccatgaggtt ccggagctcc cggatgtttc
     8461 ccggccaatc gtggccggag aggaagcgga gggctccggc cgagagctcc cgggcctggc
     8521 cgttcccgcc ggatttctgg gacaggaaga agcggcagag cccggggatg tcctcccggc
     8581 gggaccgcag gggcggcacc cggaccagga cgacggcgag ccggaaatag agatcctccc
     8641 ggaatcggcc cgccgcgatc tgggcatcca ggtccttgtt ggtcgcggcg atcacccgca
     8701 cgtcggcgga aagagattcc tggccgccga tcctttcgaa gcgcatgtcc tggatcgccc
     8761 taaggacctt ggcctgggcc gtcaggctca tgtccgcgat ctcgtccagg aaaagggtgc
     8821 ccccgtcggc cgcctcgaac ttgccgatcc tgcgggagac cgcgtccgtg aaggccccct
     8881 tctcgtgccc gaagagttcg ctctcgatca gggtgtccgg gatggccgcg cagttcaccg
     8941 ccacgaaggg cccccgggcc cggccggagg cgccgtggat ccgccgggcg accagttcct
     9001 tgccggtccc gttctccccg gtgatcagga cccgggcgtc gctcttggcc gcctggtccg
     9061 ccaggtcccg gacctcccgg atggccgcgg aatccccgat gatctcgtcc tccccgagga
     9121 ccgcgctgcg gagccggcgg ttttccgccc gcagggccgc catggacagg ccgttccgga
     9181 cggcggtcag gaggcgatcg atggaaaggg gcttctcgat gaagtcgaag gctcccaggc
     9241 ggacggcctg gacggccatg tcgatagtcc cgtggccgga gacgaccagg acctccagct
     9301 ccggcttccg cttccggatc tcccggagca catccatgcc ccccatgcgg ggcaggcgga
     9361 cgtccaggat gaccaggtcc gcccgctcgg tctccaggat gtccaggccc acgatcccgt
     9421 cctccgccag gagcacccgg tacttctcgt cctggaggat gtcgcgcacc gcggcgcgga
     9481 tggcgggctc gtcgtcgatg accaggatcg tgttcattcg ggcgatttcc tcaggagtcg
     9541 aaggggaggt ccaggaagaa gacggtcccc gcgccgggct cggattcgaa ccgcacccgt
     9601 cccccgtggt cctggacgat gcgctcggtg atcgacaggc ccagaccggt ccccgcccgc
     9661 ttggtcgtga aataaggtac gaagatgcgg tcccggttct ccggcgggat cccccggccc
     9721 gtatctcgta tctggagcct acaatagcgg gattccgcgg ccttgacaag gtcggagcgg
     9781 aacgtgaccg atcccttccc gtccatggcg tccagtgcgt tggagatcaa gttctccagg
     9841 gcctggcgca tccgggtccg gtccaccctc agcaccaggt cgggatccac gttctcggcg
     9901 tcgaacctgg cctccgggaa ggaggcgcgg tagggggagc aggattcctc caccagggtc
     9961 cggaggttgg tccagtcc
//
LOCUS       DAOTEI010000133         9673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_4971_length_9673_cov_5.274173, whole genome shotgun sequence.
ACCESSION   DAOTEI010000133 DAOTEI010000000
VERSION     DAOTEI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9673
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4971_length_9673_cov_5.274173"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="P5313_12180"
     CDS             <1..317
                     /locus_tag="P5313_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81168.1"
                     /translation="LPMEERMRLYKEKYGKHLAENPEPKAQGRKKGRRRRGRHPGAEG
                     PGGGPQNGAPSRTESGGTPSDSTGPSSAGAPDSSSQPGGADRSGLFGRIFNGSRKEED
                     RD"
     gene            593..3130
                     /gene="glgP"
                     /locus_tag="P5313_12185"
     CDS             593..3130
                     /gene="glgP"
                     /locus_tag="P5313_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRY81169.1"
                     /translation="MRIITHTVKPKIPEPLLPLEELAHNLWLSWNFDAVMLFIRLDYD
                     IWLQSRQNPAKMLGMVSQERYEQVAQDDSYLAALGDVYQRFTRYRDGETWYKGSHEDR
                     VAYFSMEYGLDISLPTYSGGLGILSGDHIKTSSDLGLPLVGVGLLYRQGYFKQYLNPD
                     GYQQEEYPENDWYNMPVNRCKKPDGSPVMISVDNAGRVVTAQVWEVKVGRASLYLLDT
                     NIDENSPEDRLITATLYGGDKEMRIRQEILLGIGGIRALRALGINPAVTHMNEGHSAF
                     LALERLREVMQAKGFAWKEALQTLWPTNIFTTHTPVPAGNERFGIDLMEKYFRGLCHE
                     LKMDWKEFLSLGRERPEDDSEPFCMTVFALKVSAYANGVAALHGKVSRQMWRGIWNGL
                     SVEEVPIGHVTNGVHPRTWISNNMLELLDRYFGPRFYDEPTDLDIFDRIDRVSDEELW
                     RTHERRRERLVVFARDRIRKQMQRSGVSESRLFHAEDALSPSILTISFARRFATYKRG
                     NLLLRDPERLIRLLQDKDRPIQLIFAGKAHPHDLPGKELIKELVHFSRREDVQSRMVF
                     LEDYDMTMARYMTSGSDLWLNTPKRPLEASGTSGMKAGMNGVLNCSVLDGWWDEGYEP
                     GIGWAIGRGEEYQDDELQNEVESKALFDLLEREIVPMFYARGRDNLPREWIRSMKASM
                     RQVGKKFSCHRMLMEYADKYYFPALDNYRAMAEKDFAPAKDVAAYLEKARREWPRLAV
                     LDIQTDAQPIMERGDTVNVTARVELAGLAPGDVAVELYHGGMSSQGEIVKPLRAEMAP
                     GKQAGQGWNYHVAVTCAVTGQHGYSVRILPKHPALVHPFLPGLVRWA"
     gene            3191..4639
                     /locus_tag="P5313_12190"
     CDS             3191..4639
                     /locus_tag="P5313_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY81170.1"
                     /translation="MGSKNFEGGAYDVAIIGAGVCGANIARRLSQYELKVILLDKEAD
                     VSFGTSKANSGIVHGGFHHSPKYLKARLEVQGALMFDRLHRELGFPFRRCGIVVAALH
                     EDEMRVVDQLYRQGVENGVIGIEMCSRERMLELEPKLNPDTVGGLYAPAGGIVEPYRF
                     VFSLVESAQRNGVEVRTSFKAERAEREGDLWRIRAEDGASLSARYVVNAAGLFADEVS
                     RAFGAEDYTIRARKGEYYLLDRMTRARPDRVVFPVPTSVSKGMLVVPTVEGTVLIGPT
                     AEMTDDKSDTATTRERLEMILGNARAMIPAISENDVITSFAGQRPVLEEDFHIEPSGK
                     VPAFIHVAGIQSPGLTASPAIGEYVKDLLKEAGLRLVEKPGYDPVLPDRPRMFDIDHY
                     QADALVARDPAYGNIVCRCEKVSEAEIVAAIRAGHTTLDGIKYFTRAQMGRCQGGFCT
                     YKILKILMRETGMSWDRITKRGGGSELLSGRL"
     gene            4636..5886
                     /locus_tag="P5313_12195"
     CDS             4636..5886
                     /locus_tag="P5313_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY81171.1"
                     /translation="MRELSYDAAVIGGGAAGMAAALEIRAAGLSCVVLEREEHLGGIL
                     LQCVHNGFGLIEFDEELSGPEFAERMEEKFRASGADAYLGVTVMDVEDPGDRKVLFGY
                     SAEHGILRVSVRAVVLAMGCRERNRGNIRIPGARPAGVYTAGLAQRLVNMEGYLPGKD
                     VVIIGSGDIGLIMARRMSWAGARVHGVVEILPYPSGLTRNIVQCLHDFGIPLYLSHLT
                     SGIFGGDRVESVEIAPIEDGAVRFEKAFRIPCDTVLLSVGLVPENELSKKMGVELNPM
                     TNGPWTDSMMMTNVPGIFAAGNVLHVHDLVDYVAEEARRAGAACARWIRGERPGRELR
                     VKAGPNVRYAAPCRVGPEAPNKLYLRTLIVKNDAVLEVRLDNRVIQSRKKPHVQPSEM
                     ISVTLDPKDLADAGPDSTIEIAIL"
     gene            5897..6253
                     /locus_tag="P5313_12200"
     CDS             5897..6253
                     /locus_tag="P5313_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HRY81172.1"
                     /translation="MPEELICITCPLGCRLSVERLPAGGLSVSGNRCTRGARYAEEEL
                     LAPKRVVTATCRALPADRCRVPVRTAAPFPKDRVEELIRVLYSLEVRLPVKRGDVLVP
                     DALGTGIPVIATRTLD"
     gene            6387..6725
                     /locus_tag="P5313_12205"
     CDS             6387..6725
                     /locus_tag="P5313_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81173.1"
                     /translation="MTNLPAASQIIVSVIPIVGIVAGAVVIFFYLLWNHKEKAVLIER
                     GEYRRPVFDLDTFCLLAGLLLAGVGTALTAVLALMNGLGYEILGGAIPLAVGASFLAF
                     YGFRAARGRR"
     gene            6722..7276
                     /locus_tag="P5313_12210"
     CDS             6722..7276
                     /locus_tag="P5313_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY81174.1"
                     /translation="MTEAPGVPDEVLVAQVLDGQAAAFGELTRRHQRRVLRIGYGFFR
                     NPEDAADFAQDVMIKAYTALATFRGAAKFSTWLTRIAYNTAINEARRKPRYDPLEVDP
                     VDTRELAPEDRHIREETVAALRKAMGDLPQKYAVCLDMYFYLGMKYGEISEATGFPVN
                     TIKSHVFRAKRELRAALGAAEVDA"
     gene            7273..7725
                     /locus_tag="P5313_12215"
     CDS             7273..7725
                     /locus_tag="P5313_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81175.1"
                     /translation="MNCEVFEERYDRAEDGAKLSPALALHLARCPRCAARVDLRRRAL
                     ELYRLPGTGPDIASRVLAALPFLPRPHRSVSLRDWILAGLALSASVILVPAQRVFSLV
                     IEEYGNRWMLPFVLVFGLAVSVFGALFIGTHMDELSGLVGRPREKPAR"
     gene            complement(7833..8366)
                     /locus_tag="P5313_12220"
     CDS             complement(7833..8366)
                     /locus_tag="P5313_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY81176.1"
                     /translation="MKGNRMYVDIGSMLDEIFDAAKNFGEEMQDKFGPGGAPRGPWFH
                     GPDFDENTDWYPNFSYPPMNIFLTPDRTLVFEFAVAGFDEKDISLSFQGDYMLFSARI
                     GLEAAPEDGVRFIKRRLKLKDIEKQKYYVPADKFAQEKVKAVFKNGILRVTVPPKEEM
                     ETAEGIKIEIVKEPDSN"
     gene            complement(8565..>9673)
                     /locus_tag="P5313_12225"
     CDS             complement(8565..>9673)
                     /locus_tag="P5313_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRY81177.1"
                     /translation="GFDFILVGHDHAGWDGMGFDPATKKKMWVKDPAGKVVPIYGALA
                     GAAAVPVVEVSLRWDRPNKTWIKEVNGTLLALKDRPADPDFLAKFDPYKAELMAWVER
                     PIGKMSGKITSDVAMFGDSAFVDLIHKIQLELCADPAMGLKPAQISIAAPLDMYAYLP
                     TTADGTIKVRDMFSLYKYENFLYTMDLTGEQIKKFMEFSYGGWFNTMAGPEDDLIAFA
                     KDKEGKLIFNARYNSYDTVTRAYNYDSFAGLNYTVDVSKPVGSRVTITGMSDGKPFDP
                     AKTYSVAINSYRGSGGGGHLTKGVGLDAKKLARLEMVTSSTTKDLRYYMLKWFEKQTG
                     VIAPASDNNWKVLPEDWAAAGAKKNYSYLYPPAK"
BASE COUNT         1608 a         3223 c         3256 g         1586 t
ORIGIN      
        1 ccctgcccat ggaggagagg atgcggctgt acaaggagaa gtacggcaag cacctggccg
       61 agaatccgga gccgaaagcc cagggccgca agaaaggccg caggcggcgg ggcaggcatc
      121 ccggagccga gggaccgggc ggcgggcccc agaacggggc tccgtcgcgc acggaatccg
      181 gaggaacccc ctccgattcg accggcccgt cctccgcggg cgcccccgac tcctcctccc
      241 agccgggcgg cgcggatcga agcggtctgt tcggccgcat cttcaacggc tcccgtaagg
      301 aagaagaccg ggactgatcc ttaccgaaag gcttggaacc atcggtcgga caggatgtac
      361 aggattaaaa ccggatagac aggatgggaa tggggaattg accacctcct catgtatcct
      421 gtccatcgtt tcttcatcct gttcatcctg tcaaatatgc cggaagtctg ctgtagttgt
      481 actaaccggc actgtttctt gacaccccgg actccactcg actaggctta acccagccgc
      541 gggggctttc cgcccgacgg atacccccct cccccgccgc taggagtccc ccatgaggat
      601 catcacccac acggtcaagc cgaagatccc cgagcccctc ctgcccctgg aggaattggc
      661 ccataatctc tggctgtcct ggaatttcga cgcggtcatg ctcttcatcc ggctggacta
      721 cgacatctgg ctccagagcc gtcagaaccc ggccaaaatg ctggggatgg tctcccagga
      781 gcggtacgag caggtggccc aggacgattc ctatctggcg gccctggggg acgtgtacca
      841 gcgcttcacc cgataccggg acggggagac ctggtacaag gggagccacg aggaccgggt
      901 ggcctacttc tccatggagt acggcctgga catctccctg cccacctact ccgggggcct
      961 ggggatcctc tcgggggacc acatcaagac cagctccgac ctgggcctgc ccctggtggg
     1021 ggtgggcctc ctgtaccggc agggctactt caagcagtac ctcaaccccg acggctacca
     1081 gcaggaggag taccccgaga acgactggta caacatgccg gtcaaccgct gcaagaagcc
     1141 cgacggctcc ccggtcatga tctccgtgga caacgccggc cgggtcgtga ccgcccaggt
     1201 ctgggaggta aaggtgggcc gggcctccct ctacctcctg gacacgaaca tcgacgagaa
     1261 ctccccggaa gaccggctca tcaccgccac cctctacggc ggggacaagg agatgcggat
     1321 ccgccaggag atcctcctgg ggatcggcgg gatccgggca ctgagagccc tggggatcaa
     1381 tcccgcggtc acccacatga acgaggggca ctccgccttc ctggccctgg agcgcctgcg
     1441 ggaggtcatg caggccaagg gcttcgcctg gaaagaggcc ctccagaccc tctggcccac
     1501 gaacatcttc accacccata cccccgttcc cgcgggaaac gagagattcg gcatcgacct
     1561 catggagaag tacttccggg ggctctgcca cgagctcaag atggactgga aggaattcct
     1621 gtccctgggc cgagaacgtc cggaggacga ctccgagccc ttctgcatga ccgtcttcgc
     1681 cctcaaggtc agcgcctacg ccaacggggt ggcggcgctg cacggcaagg tctcccggca
     1741 gatgtggcgg gggatctgga acggactgtc cgtggaggag gtccccatcg ggcacgtcac
     1801 gaacggcgtg cacccccgga cctggatctc caacaacatg ctggagctcc tggaccggta
     1861 cttcgggccc cggttctacg acgaacccac ggacctggac atcttcgacc ggatcgaccg
     1921 ggtctccgac gaggagctct ggcggaccca cgagcgccgc cgggaacgcc tggtggtctt
     1981 cgcccgggac cggatccgga agcagatgca gcgctccggg gtttccgaga gccgcctctt
     2041 ccacgccgag gacgccctct cgccctccat cctgaccatc agcttcgccc gcaggttcgc
     2101 gacctacaag cggggcaacc ttctcctacg ggacccggag cgcctgatcc gcctgctcca
     2161 ggacaaggac cggcccatcc agctgatctt cgccggaaag gcccaccccc acgacctgcc
     2221 cggcaaggag ctgatcaagg agctggtcca cttctcccgc cgggaggacg tgcagagccg
     2281 catggtcttc ctggaggatt acgacatgac catggcccgg tacatgacct ccggctcgga
     2341 cctctggctc aacaccccca agcgccccct ggaagcctcg ggcaccagcg gcatgaaggc
     2401 cgggatgaac ggggtcctca actgctccgt cctggacggg tggtgggacg agggctacga
     2461 gcccgggatc ggctgggcca tcggccgcgg ggaggagtac caggacgatg agctccagaa
     2521 cgaggtggag agcaaggccc tcttcgacct cctggagagg gagatcgtcc cgatgttcta
     2581 cgcccggggc cgggacaacc tcccccggga gtggatccgc tccatgaagg cctccatgcg
     2641 gcaggtgggc aagaaattct cctgccaccg gatgctgatg gagtacgcgg acaagtacta
     2701 cttccccgcc ctggacaact accgcgccat ggcggagaag gacttcgccc cggccaagga
     2761 cgtcgcggcc tacctggaga aggcccggcg ggagtggccc cggctggcgg tcctggacat
     2821 ccagaccgac gcccagccca tcatggagcg gggggacacg gtcaacgtga ccgcccgggt
     2881 ggagctggcg ggcttggctc ccggggacgt ggccgtggag ctctaccacg gcggcatgtc
     2941 cagccagggc gagatcgtga agcccctgcg ggccgagatg gccccgggta aacaggcggg
     3001 acagggctgg aactaccacg tggcggtgac ctgcgccgtc accggccagc acgggtactc
     3061 ggtccggatc ctgcccaagc acccggccct ggtccatccc ttcctgccgg gactggtccg
     3121 gtgggcctga gaagcgcgcc gggccgcccg ggcggccgcg catccgatac ctgaacgaga
     3181 ggaacggcga gtgggatcca agaatttcga gggcggcgcc tacgacgtcg cgatcatagg
     3241 ggcgggggtc tgcggggcga acatagcccg caggctctcc cagtacgaac tgaaggtcat
     3301 cctcctggac aaggaagcgg acgtctcctt cggcacctcc aaggccaact ccggcatcgt
     3361 ccacgggggc ttccaccaca gcccgaagta cctcaaggcg cggctggagg tccagggcgc
     3421 cctgatgttc gaccggctcc accgggaact gggcttcccc ttccggcgct gcgggatcgt
     3481 ggtggccgcc ctccacgagg acgagatgcg ggtggtggac cagctctacc ggcagggggt
     3541 ggagaacggg gtcatcggga tcgagatgtg ctcccgggag cggatgctgg aactggagcc
     3601 caagctgaac cccgacaccg tggggggcct ctatgccccg gcggggggga tcgtggagcc
     3661 ctaccgcttc gtcttctccc tggtggagtc cgcccaacgg aacggggtgg aggtgcgcac
     3721 gtccttcaag gccgaacggg ccgagcggga gggggacctc tggcggatcc gggccgagga
     3781 cggggcctcc ctttcggcgc gctatgtcgt gaacgccgcg gggctattcg cggacgaggt
     3841 ctcccgggct ttcggggccg aggactacac gatccgggcc cggaaggggg agtactacct
     3901 cctggaccgg atgacccggg cccggccgga ccgggtggtc ttcccggtgc ccacctcggt
     3961 atccaagggg atgctcgtgg tgcccaccgt ggaggggacg gtcctgatcg gccccaccgc
     4021 cgagatgacc gacgacaagt ccgacacggc caccacccgg gagcgattgg agatgatcct
     4081 gggcaacgcc cgggccatga tcccggcgat ctcggagaac gacgtgatca cctccttcgc
     4141 gggccagcgg cccgtcctgg aggaggactt ccacatcgag ccctccggga aggtgcccgc
     4201 cttcatccac gtggcgggga tccagtctcc ggggctcacc gccagccccg ccatcgggga
     4261 atacgtgaag gacctcctca aggaggcggg gctccggctg gtggagaagc cgggctacga
     4321 cccggtcctg ccggaccgtc cccggatgtt cgacatcgac cactaccagg cggacgccct
     4381 ggtggcccgg gatcccgcct acggcaacat cgtctgccgc tgcgagaagg tcagcgaggc
     4441 ggagatcgtg gcggccatcc gggccggcca taccaccctg gacggtatca agtacttcac
     4501 ccgggcccag atgggccgct gccagggagg gttctgcacg tacaagatcc tcaagatcct
     4561 gatgcgggag accggaatga gctgggaccg gatcaccaag cggggaggcg ggagcgagct
     4621 gctctcgggg cgcctatgag ggagctttcc tacgacgccg ccgtcatcgg cgggggggcc
     4681 gcgggcatgg ccgccgccct ggagatccgg gccgccgggc tgtcctgcgt ggtcctggag
     4741 cgggaggagc atctgggagg catcctcctc cagtgcgtgc acaacggctt cgggctcatc
     4801 gagttcgacg aggagctctc gggtccggaa ttcgcggagc ggatggagga aaagttccgg
     4861 gcctccgggg cggacgcgta cctcggcgtc acggtcatgg acgtggagga cccgggagac
     4921 cggaaggtcc tcttcggcta ctccgcggag cacgggatcc tccgggtgtc cgtccgggcc
     4981 gtggtcctgg ccatgggctg ccgggagcgc aaccggggga acatccgcat tcccggggcc
     5041 cgtccggcgg gggtctacac cgcggggctg gcccagcgcc tggtgaacat ggagggctac
     5101 ctccccggca aggacgtggt gatcatcggg tccggtgaca tcggcctcat catggcccgt
     5161 cggatgagct gggcgggggc ccgggtccac ggggtcgtgg agatcctgcc ctacccctcg
     5221 gggctgaccc gcaacatcgt ccagtgcctc cacgacttcg gcatccccct ctacctctcc
     5281 cacctgacct cggggatctt cggaggggac cgggtcgagt ccgtggagat cgcccccatc
     5341 gaggacgggg cggtgcgctt cgagaaggcc ttccggatcc cctgcgacac ggtcctgttg
     5401 tccgtgggcc ttgtgccgga gaacgagctg tccaagaaaa tgggggtgga gctcaacccc
     5461 atgaccaacg gtccctggac ggactccatg atgatgacca acgtccccgg gatcttcgcg
     5521 gcgggaaacg tcctgcacgt ccacgacctg gtggactacg tggccgagga ggcccggcgc
     5581 gcgggggccg cctgcgcccg gtggatccgg ggcgaacgtc ccggccggga gctccgggtc
     5641 aaggcgggcc ccaacgtccg ctatgcggcc ccctgccggg tcggcccgga ggcaccgaac
     5701 aagctctacc tgcggaccct gatcgtgaag aacgacgcgg tcctggaggt ccgcctggac
     5761 aaccgcgtca tccagagccg taagaagccc cacgtccagc cctcggagat gatctccgtg
     5821 accctggatc ccaaggacct ggcggacgcc ggaccggatt ccacgatcga gatagccata
     5881 ctgtgaggat agcccgatgc ccgaagagct gatctgcatc acctgccccc tgggctgccg
     5941 cctctcggtg gagcgcctgc ccgcgggagg actctccgtt tccgggaacc gctgtacccg
     6001 gggcgcccga tacgcggagg aggagctcct ggcccccaag cgggtggtca cagccacctg
     6061 ccgcgccctg cccgcggacc ggtgccgggt gcccgtgcgc accgccgcgc cctttcccaa
     6121 ggaccgggtg gaggaactga tccgggtcct ctactccctg gaggtccggc taccggtgaa
     6181 gcggggggac gtgctggtgc ccgacgccct gggaacgggg attcccgtga tagccacccg
     6241 gaccctggat tgaggaccgg gcgcgtaccg gccgcccggg cttcgcaacc ggatgccggc
     6301 tttcgcgtcg aactaggtgc gggtccgttc cgggggcata cggccccgcg gggagcccgt
     6361 gaaatcccgg aacccgaggt gcaaccgtga cgaaccttcc cgcggcttcc cagatcatcg
     6421 tctccgtcat ccccatcgtg gggatcgtcg ccggggccgt cgtgatcttc ttctacctcc
     6481 tgtggaacca caaggaaaag gccgtcctga tcgagcgggg cgagtaccgt cgcccggtct
     6541 tcgacctgga caccttctgc ctcctggcgg ggctgctcct ggcgggggtg ggcacggcgc
     6601 tgaccgccgt cctggccctg atgaacgggc tggggtacga gatcctgggc ggggccatac
     6661 ccctggcggt aggagcctcc ttcctggcct tctacggctt ccgggccgcc cgcggaagga
     6721 gatgacggag gcgccgggcg ttccggacga ggttctggtg gcccaggtcc tggacggaca
     6781 agccgccgct ttcggggagc tcacccggag gcaccagcgc cgggtcctgc gcatcgggta
     6841 cgggttcttc cggaaccccg aggacgcggc ggacttcgcc caggacgtga tgatcaaggc
     6901 ctacacggcc ctggcgacct tccggggcgc ggccaagttc tccacctggc tcacccggat
     6961 cgcctacaac acggccatca acgaggcccg tcggaagccc cggtacgatc ccttggaagt
     7021 cgaccccgtg gacacccggg agctggcccc ggaggaccgg cacatccggg aggaaaccgt
     7081 ggcggccctt cgaaaggcca tgggggacct gccccagaag tacgcggtct gcctggacat
     7141 gtatttctat ctgggcatga agtacgggga gatcagcgag gccaccggat tccccgtgaa
     7201 cacgatcaag tcgcacgtct tccgcgccaa acgggagctg cgggcggcgc tgggcgccgc
     7261 ggaggtggac gcatgaactg cgaagtcttc gaggaacggt acgaccgggc ggaggacggc
     7321 gccaagctct ccccggccct ggccctgcac ctggcccgct gtccccgctg cgcggcccgg
     7381 gtggacctgc gccgccgggc cctggagctc taccgtctcc cgggcaccgg tccggacatc
     7441 gcctcccggg tcctggccgc cctgcccttc ctgccccggc cgcaccggtc ggtttccctg
     7501 cgggactgga tcctggcggg cctggccctc tccgcgtccg tgatcctggt gcccgcccag
     7561 agggtcttct ccctggtgat cgaggagtac gggaaccgct ggatgctgcc cttcgtcctg
     7621 gtgttcggcc tggcggtctc ggtcttcggg gcactcttca tcgggaccca catggacgag
     7681 ctgtccggcc ttgtcggccg cccccgggag aagccggcgc ggtaggggcg gagaccgggg
     7741 cggcccgcgc cgcacggacc gccccggtac gtccggtcga taaaaaaacg ggccgcccct
     7801 gccgggcggc ccgttcatat cctgtgttcg gctcagttcg agtccggttc cttcacgatc
     7861 tcgatcttga tgccctcggc ggtctccatc tcctccttgg gggggacggt cacccggagg
     7921 atgccgttct tgaacaccgc cttgaccttt tcctgggcga acttgtccgc ggggacatag
     7981 tacttctgct tttcgatgtc cttcagcttg aggcggcgct tgatgaagcg caccccgtcc
     8041 tcgggcgcgg cctcgaggcc gatgcgcgcg gagaagagca tgtagtcccc ctggaaggag
     8101 aggctgatgt ccttctcgtc gaatcccgcc acggcgaact cgaacaccag ggtccggtcg
     8161 ggggtcagga agatgttcat gggcggatag gagaagttgg ggtaccagtc ggtgttctcg
     8221 tcgaaatccg gcccgtggaa ccagggaccg cggggggccc cgccgggacc gaacttgtcc
     8281 tgcatctctt cgccgaagtt cttggccgcg tcgaagatct cgtccagcat ggacccgatg
     8341 tccacgtaca ttctgttgcc cttcatagca ttcctccgtt ctgcgaggat cgtcccggga
     8401 ccttcccggt ctcacggggc gttgatcgcg ccctccatac ggagcggcgc cgacggaccg
     8461 ggcatccttg ggggaccgtc cgttccggta atgttaaaat agcgataatt ctcaaaaaag
     8521 acaaccgccg ccctcaaggg cggcggtata tgtttgcggc gtgtttactt ggccggcgga
     8581 tacaggtagg aatagttctt cttggccccc gcggccgccc aatcctcggg aagcaccttc
     8641 cagttgttgt ccgaggcggg ggcgattacg cccgtctgct tctcgaacca cttgagcatg
     8701 tagtaccgca ggtcctttgt ggtggaggag gtcaccatct ccaggcgggc cagcttcttg
     8761 gcgtccaggc ccacgccctt ggtcagatgc ccgccgccgc cggagccgcg gtaggagttg
     8821 atggccacgc tgtaggtctt ggccgggtcg aagggcttgc cgtcggacat gccggtgatc
     8881 gtgacgcgac tgcccaccgg cttggagacg tccaccgtgt agttcaggcc cgcgaaggag
     8941 tcgtagttgt acgcccgggt gacggtgtcg taggagttat accgggcgtt gaagatcagc
     9001 ttgccctcct tgtccttggc gaaggcgatg aggtcgtcct cgggaccggc catggtgttg
     9061 aaccatccgc cgtaggagaa ctccatgaac ttcttgatct gctcccccgt caggtccatg
     9121 gtgtacagga agttctcgta cttgtagagg ctgaacatat cccggacctt tatcgtcccg
     9181 tccgcggtgg tgggaaggta ggcgtacatg tccagggggg ccgcgatgga gatctgggcg
     9241 ggcttgaggc ccatggcggg atccgcgcag agctccagct ggatcttgtg gatcaggtcc
     9301 acgaaggcgg aatccccgaa catggccacg tccgaggtga tcttgccgct catcttgccg
     9361 atgggcctct cgacccaggc catgagctcc gccttgtagg ggtcgaactt ggccaggaag
     9421 tccgggtccg cgggccggtc cttgagggcc aggagggttc cgttcacctc cttgatccag
     9481 gtcttgttcg gcctgtccca gcgcagggag acttccacca cggggacggc ggcggctccg
     9541 gccagggcgc cgtagatggg cacgaccttt ccggccgggt ccttgaccca catcttcttc
     9601 ttggtggccg ggtcgaagcc catgccgtcc cagcccgcgt ggtcgtgccc gaccaggatg
     9661 aagtcgaagc ccg
//
LOCUS       DAOTEI010000134         9629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5006_length_9629_cov_4.774076, whole genome shotgun sequence.
ACCESSION   DAOTEI010000134 DAOTEI010000000
VERSION     DAOTEI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_9629_cov_4.774076"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="P5313_12230"
     CDS             <1..1183
                     /locus_tag="P5313_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRY81178.1"
                     /translation="LGLLLPSGSPTDATLGILFELAIRGGRYIFLPLIFFSVPIAVFE
                     LYEDRKLGPTLGKAALISAVSVFAASILGAVGAFVLQPDRIPLVSETSAVEPLASAGD
                     IILGLFPRNAFQALVSGGDGLAPLLILAVILGLAFSFDQAVTKPVIGMFDSLSRILYQ
                     INSFFVEILAVFSIAAAAYNARAFRTVLELSLYRSLLLSVAAEVLFTVLIVLPGILYF
                     AGGRKNPFRILYGLAGPALAAVVSGDLFFCAGTLVKHGKENLGIRRRSGTLTLPVALL
                     LGRAGTALVTSTSFIVVLNSFSNIGLTAENLLWLLLFVPLSAFLLGSAPGRGPAAALA
                     LLSGLYGRGFETGHLLLVQAAIPLLLAGAFLDAVWAGCVSWLTARSEGLAAEREVRRY
                     I"
     gene            1223..1669
                     /locus_tag="P5313_12235"
     CDS             1223..1669
                     /locus_tag="P5313_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY81179.1"
                     /translation="MNLRTVLNEKSIALHLKGRTKDEIIRELLEVLVKNGSVSSFEKA
                     YQGILERERKMSTGMKHGIALPHGKTDAVKDLAACVGVSDFPIDYESLDGKPCRIFIM
                     LLSPPAKTGPHLQFLAEVSLLFRSAAKREALLAAENPGAVLRALSE"
     gene            complement(1774..2040)
                     /locus_tag="P5313_12240"
     CDS             complement(1774..2040)
                     /locus_tag="P5313_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRY81180.1"
                     /translation="MKVKPLGDRVLIKIQESEAKTAGGIIIPQTAQEKTQTGVVVAVG
                     TDADVIKVKAGEKVMYDKYAGTQIKVEGADHLIVKMSDILAVID"
     gene            2217..4166
                     /gene="ftsH"
                     /locus_tag="P5313_12245"
     CDS             2217..4166
                     /gene="ftsH"
                     /locus_tag="P5313_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRY81181.1"
                     /translation="MPGKKKTSWDKNLMKPRTPGGKVPNLPPGFNGWRLSLIYMGMTL
                     LMIILFNWYMVSSDETVIPFSDFKSRIDSGEIVRVEMDSSWYRGYVHSESTAEGQPSL
                     WPRRMVSSPALPADKLAYKTVPVYDPDFTKLLDSRAVVYSSVPREGNAMVSILLNWVL
                     PFGAIFLLWRLLQKRLSNVNSNVLAFGQNKAMIVAEGDVKARFTDVAGVDEAKEELVE
                     VVDFLKSPKKYTDIGGKIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFRMSGAEFVEM
                     FVGVGASRVRDLFRQAREKAPCIIFIDELDAIGKSRISGPMGGNDEREQTLNQLLVEM
                     DGFDATSGLIILAATNRPDVLDPALLRPGRFDRQVMVDRPDLIGRQAILAIHAKAVKL
                     DEAVDLGDVARSTPGFVGADLANIVNEAALLAVRGGRRKVAQRDFEAAIEKVVTGLEK
                     KSRVMNPEERKAIAYHEAGHALVAAFTEGSDPVQKVSIVPRGFGALGFTLQMPTEDRY
                     VVTEGQLLGQIDVLMGGRAAEEIVFGRISTGAASDITKATDIARRMITDYGMSERFRN
                     VALTKRGTGMGGVQDAFLTREYSEETQRYVDDEVAKIIAERYALALKTLRDRKALLDT
                     VSAELLEKETLDAARFRELVGTTTV"
     gene            4292..5623
                     /locus_tag="P5313_12250"
     CDS             4292..5623
                     /locus_tag="P5313_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRY81182.1"
                     /translation="MWADVILVVYAAILLGIALLSLKYNTNLENFLVAGHKQRKILVV
                     ASMLASTIGGGITIGTVANAYRIGFPAFWFVAAGGIAHFLQGALLSERVRASEALTLP
                     DLANRHLGPAVRMEISLIILVTWIGISAAQFVAAARIITTLTGMPHAGAVASAAAFIV
                     VYTLIGGQKSVLRTDLFQFGILAGAVVLILSYLFLAKPPAPGTVRIDLFTEAFGPLDL
                     VYYVVVMGGSYFICPMMFSRILSADSPANARKSSFLSGTGMLFFAFAITFVGLWARAS
                     LPDLGGLDPLNSIARNHVPSALGIVLIVGLLAAILSTADTVLITAAGILEHDVFKGTR
                     VAGVRMWTVAVGAVGAGIALFQTDVIAMLMKTYNGYTAGLVPALFVALMLPAGRRVRP
                     LWGFAAIAGGYALGLAGSITGSKVLPLVGMAFSAGVALAGIRPVEPSNTRA"
     gene            5710..6696
                     /locus_tag="P5313_12255"
     CDS             5710..6696
                     /locus_tag="P5313_12255"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRY81183.1"
                     /translation="MAVLIRSTGSFVPDNRYSNDQLSAIVDTTDEWIRSHTGIGARHI
                     APDGTMTSDLALGAAKSALEKAGIGPEEVDYIAVATASPDYFGFPATACIVQDKLGAS
                     KAAAFDVVAGCTGFIYALDTVSAMLEARKARYGLVIGAETLSRIADWADRSTCVLFGD
                     GAGCALLENSQAETDRGLKASILGSDGGGALDLYLAQGMRDKTFDRGEPYQDFPKIVM
                     NGKNVYNFAVKAITKLIGDLLEKSGIPLPEFRWIVPHQANLRIVQAAAKRFGLPEERF
                     YLNIQEYANTSAASVPIALNEIAEKSLVKAGDPVMLLGFGAGLTFGGSVLRY"
     gene            6783..8030
                     /gene="fabF"
                     /locus_tag="P5313_12260"
     CDS             6783..8030
                     /gene="fabF"
                     /locus_tag="P5313_12260"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRY81184.1"
                     /translation="MDTKRRVVVTGMGVVSPVGNDLASFWDAVKNGRSGIGPVTRFDA
                     SRLEARIAGEVKDLDVSLWMDKKEARKMALFTQYAVSAAAQAWAQAGMDGASTPRDRI
                     AIVVGNGIGGEEIHCESHAKMLESGPGRIPPMTIPLMIMNEAAGNIALRFQIKGPAYT
                     QATACASGTDALGQALDMIRAGRCDVAIAGGTEGAITEFAIGGFCRIQALSTKRNDEP
                     AKASRPFDKDRDGFVIAEGAGMMILESWEHAKARGAAILAEFAGYGASCDSYHLTAPD
                     PEGSGGALAIRRAIEDAGLAPSDIQYYNAHGTSTPINDPTETLMVKKAFGDHAGKLKI
                     SSTKSVTGHMIAAAGAVEAIVCVQAIREGFCPPTINLDEPDPACDLDYVPNKGVALPV
                     RAAASASLGFGGHNGCVVFRRAE"
     gene            8027..8476
                     /gene="fabZ"
                     /locus_tag="P5313_12265"
     CDS             8027..8476
                     /gene="fabZ"
                     /locus_tag="P5313_12265"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRY81185.1"
                     /translation="MSGKSAETRAEVEALLPHRDPFRFVDSVRIEGEKILGKKLWTGN
                     EFFFPGHFPGHPVVPGVILLETMAQCGGVGVKLQGIVPAGTFFFAAMDGIKFRKPVYP
                     GDTFEIEIVNLRASSRAVKQQGMGYVNGEKAVEASWLCLVGVEGGPS"
     gene            8482..>9629
                     /locus_tag="P5313_12270"
     CDS             8482..>9629
                     /locus_tag="P5313_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HRY81186.1"
                     /translation="MIRNNICMNAHGPGCEALTEGWIARALEGKPAIQSAVGTSAKKY
                     RSVLVLGGSTGYGLASRIVAAFCLGADTVSVSFEREPSDKRAGTPGWYNNRAFDRAAV
                     GAGLRSWGLDADAFADSTREAVIALAREKGLIFDLVVYSLASPVRTDPKTGQMYRSVI
                     KPLDRPYEGINLDVFSGKISRATVQPASAEEAEHTVKVMGGEDWELWIRALRNTGVLA
                     PDAETLAYTYIGPEHSWPIYRSGALGKAKAHLEATARTLDSLLKPGGGAAWAAVMKAV
                     VTRASAVIPVIPLYVSTLFKVMKEKGVHEDCLDQGLRLFRDRLLKKDVPTDPERRIRL
                     DDLEMGLEVQAETSRRMKTVTEENAETVADIEGFRTDFLQAHGFAVPGV"
BASE COUNT         1589 a         3286 c         3145 g         1609 t
ORIGIN      
        1 cctgggtctc ctcctgccct ccggctcccc gacggacgcc accctgggga tcctcttcga
       61 gctagcgatc cggggcggac ggtacatctt ccttcccttg atcttcttct ccgtcccgat
      121 cgcggttttc gagctctacg aggacaggaa gctgggtccg accctgggca aggccgccct
      181 gatctcggcc gtctcggtct tcgccgcgtc gatcctgggc gccgtgggcg ctttcgtgct
      241 tcagccggat cgcattcccc tggtcagcga gacctccgcg gtggagcccc tggcctcggc
      301 gggggacatc atcctggggc tgtttccccg caacgccttc caggctctcg tctccggcgg
      361 ggacggactg gcccccctgc tcatcctggc ggtgatcctg ggtctggcct tttccttcga
      421 ccaggcggtc accaagcccg tgatcgggat gttcgactcc ctctcccgga tcctctacca
      481 gatcaactcc ttcttcgtcg agatcctggc cgtcttctcg atcgccgcgg cggcgtacaa
      541 cgcccgggcc ttccggacgg tcctggagct gtcgctgtat cggagtctgc tgctctccgt
      601 ggccgcggag gtcctcttca cggtgctcat cgtcctgccg ggcatcctct acttcgcggg
      661 gggccggaag aatcccttcc gcatactcta cggactggcc ggaccggccc tggccgcggt
      721 cgtctcgggg gacctcttct tctgcgcagg aaccctggtc aagcacggca aggaaaacct
      781 ggggatccgg cggagatccg ggaccctgac cctgcccgtc gccctcctgc tgggccgggc
      841 cggcaccgcc ctggtcacct cgacgagctt catcgtcgtc ctgaactcct tctccaacat
      901 cgggctgacg gcggagaacc ttctctggct cctcctgttc gtccccctgt ccgccttcct
      961 cctgggatcc gccccgggcc ggggacccgc ggccgccctg gccctgctct cgggcctgta
     1021 cggacggggc ttcgagacgg gacacctcct cctggtccag gccgcgatac ccctcctgct
     1081 ggcgggcgcg ttcctggacg cggtctgggc gggctgcgta tcctggctga ccgcccgctc
     1141 ggagggcctg gccgcggagc gcgaggtgcg gagatacatt tagcaaattg actttccgca
     1201 tggccgaccc tacaatcctc ccatgaacct ccgcaccgta ctgaacgaga aatccatcgc
     1261 cctgcacctg aagggcagga ccaaggacga gatcatccgg gaactcctgg aagtcctggt
     1321 caagaacggg tccgtgtcgt ccttcgagaa ggcctaccag ggcatcctgg agcgggaacg
     1381 caagatgtcc acggggatga agcacgggat cgccctgccc cacggaaaga cggacgcggt
     1441 gaaggacctg gcggcctgcg tcggcgtgtc ggacttcccg atcgactatg aatccctgga
     1501 cggaaagccc tgccggatct tcatcatgct cctctccccc cccgccaaga cgggcccgca
     1561 tctccagttc ctggcggagg tgagtctcct gttccggtcc gcggccaagc gggaagccct
     1621 tttggcggcg gagaatcccg gcgccgtcct ccgggccctt tccgaatgag ccgaagccgc
     1681 gcggcccggg cagagggcgc ggggtgaaga acaacgggcc gccagggaag agattcccgg
     1741 gcggcccgtc cgtttgcgga gcttcgcgag aggtcagtcg atgacggcca ggatgtcgga
     1801 catcttgacg atgagatggt ccgcgccttc caccttgatc tgggttccgg cgtacttgtc
     1861 gtacatcacc ttctcgcccg ccttgacctt gatgacgtcc gcgtcggttc ccacggcgac
     1921 caccacgccg gtctgggtct tctcctgggc ggtctgcggg atgatgatcc cgccggcggt
     1981 tttcgcctcg ctctcctgga tcttgatgag cacgcgatcg cccaacggtt tcactttcat
     2041 gacgtaatcc tcctgagaac tgggtctggg ttgtacgtag gataggcaaa aatatacgaa
     2101 aacctgcccg cccgggtcaa ggagtcatcg atctcccgaa ccccttcgag tggccggagg
     2161 atcatttttt cggtagattc aaaccgtaag accccatcgc cctcaaggga gaacctatgc
     2221 ccggcaagaa aaagacctct tgggacaaga atctcatgaa accccggact ccgggaggaa
     2281 aggtgcccaa cctccctccc gggttcaacg ggtggaggct gtccctcata tacatgggga
     2341 tgaccctgct catgatcatc ctgttcaact ggtacatggt ctcctcggac gagacggtca
     2401 tccccttcag cgacttcaag tccaggatcg actccgggga aatcgtacgg gtcgagatgg
     2461 attcgtcctg gtatcgggga tacgtgcact ccgagtccac ggcggaaggg cagccctccc
     2521 tctggccccg ccggatggtt tccagccccg ccctccccgc ggacaaactg gcctacaaga
     2581 ccgtgccggt ctacgatccg gacttcacca agctcctgga cagcagggcc gtggtctact
     2641 cgtccgtacc ccgggagggg aacgcgatgg tctcgatcct cctcaactgg gtattgccct
     2701 tcggggccat attcctcctg tggaggcttc tgcagaagcg gctgtccaac gtgaactcca
     2761 acgtcctggc cttcggccag aacaaggcca tgatcgtggc cgagggggac gtcaaggcga
     2821 ggttcacgga cgtggccggg gtggacgagg ccaaggagga gctggtcgag gtcgtggact
     2881 tcctcaagtc ccccaagaag tacacggaca tcggcggcaa gatccccaag ggggtcctcc
     2941 tggtgggccc cccgggaacg ggcaagaccc tcctggcccg ggccgtggcc ggggaggcgg
     3001 gggtgccctt cttccggatg agcggcgcgg agttcgtgga gatgttcgtg ggcgtgggag
     3061 cctcccgggt ccgggacctc ttccggcagg cccgggagaa ggccccctgc atcatcttca
     3121 tcgacgagct ggacgccatc ggaaagagcc ggatctccgg ccccatggga ggcaacgacg
     3181 agcgggagca gaccctgaac cagctcctgg tggagatgga cggcttcgac gcgaccagcg
     3241 ggctcatcat cctggccgcc accaaccggc ccgacgtcct ggaccccgcc ctcctccggc
     3301 ccgggcgctt cgaccggcag gtcatggtgg accgcccgga cctgatcggc cgccaggcca
     3361 tcctggccat ccacgccaag gcggtcaagc tggacgaggc cgtggacctg ggcgatgtgg
     3421 cccgctcgac cccgggcttc gtgggggcgg acctggccaa catcgtgaac gaggcggcac
     3481 tcctggctgt gcggggtgga cggaggaagg tggcccagcg ggacttcgag gcggccatcg
     3541 agaaggtggt cacggggctg gagaagaaga gccgggtcat gaatcccgag gagcggaagg
     3601 ccatagccta ccacgaggcg ggccacgccc tggtggcggc cttcacggag ggctcggacc
     3661 cggtccagaa ggtctccatc gtgccccggg gcttcggggc cctgggtttc accctgcaga
     3721 tgcccactga ggaccggtac gtggtgaccg agggccagct cctgggccag atcgacgtgc
     3781 tcatgggcgg ccgggccgcg gaggagatcg tcttcgggcg gatctccacc ggggcggcca
     3841 gcgacatcac caaggccacg gacatcgccc gcaggatgat caccgactac gggatgtccg
     3901 aacgcttccg gaacgtggcc ctgaccaagc ggggaaccgg gatgggggga gtccaggacg
     3961 ccttcctgac ccgggaatac agcgaggaga cccagcgcta cgtggacgac gaggtggcca
     4021 agatcatagc cgagcgctac gccctggccc tgaagaccct gcgggaccgg aaggccctgt
     4081 tggacacggt gtccgcggag ctcctggaaa aggagaccct ggacgcggct cggttccggg
     4141 aactcgtggg aacgacaacg gtataggaca tttcaacaaa tgtcctagta ctcgcttgac
     4201 attgtcttag tttcatcgta gcttgaccga aacgggttca agggccggcc gggaatgttc
     4261 cggccgaaac cccgcccccg gggaggtcga tatgtgggcc gatgtcatcc ttgtcgtcta
     4321 tgccgccatc ctgctgggca tagccctcct gagcttgaag tacaacacca acctcgagaa
     4381 cttcctggtc gcgggacata agcagaggaa gatcctcgtg gtcgcgtcca tgctggcctc
     4441 cacgatcggc gggggcatca cgatcgggac ggtggcgaac gcctaccgga tagggttccc
     4501 ggccttctgg ttcgtggccg ccggcgggat cgctcacttc ctccaaggcg ccctcctgtc
     4561 cgagcgggtg cgcgcctccg aggccctcac cctgccggac ctggccaacc gccacctggg
     4621 acccgcggtg cggatggaga tctccctgat catcctggtg acctggatcg gcatctccgc
     4681 ggcccagttc gtggccgccg cacggatcat cacgaccctg acgggcatgc cccacgcggg
     4741 agccgtggcc tccgcggcgg ccttcatcgt ggtctacacc ctgatcgggg gccagaagtc
     4801 ggtgctccgc acggacctct tccagttcgg catcctggcg ggggccgtcg tcctgatact
     4861 gtcctacctc ttcctggcca agccccccgc cccgggcacc gtgcggatcg acctgttcac
     4921 ggaggccttc ggccccctgg acctggtcta ttacgtggtg gtgatgggag gctcctactt
     4981 catctgcccg atgatgttct cccggatcct ttccgccgac tcccccgcca acgcccggaa
     5041 atcctccttc ctctccggga ccgggatgct cttcttcgcc ttcgccatca ccttcgtggg
     5101 actgtgggca cgcgcctccc tgcccgacct gggcggcctg gatcccctga actccatcgc
     5161 ccggaaccac gttccctccg ccctgggcat cgtcctgatc gtcggcctcc tggcggccat
     5221 cctgtccacc gcggacacgg tcctgatcac cgcggcgggc atcctggagc acgacgtgtt
     5281 caagggcacc cgggtggcgg gggtgaggat gtggaccgtg gccgtcgggg cggtcggggc
     5341 cgggatagcc ctattccaga ccgacgtgat cgccatgcta atgaagacct acaacggcta
     5401 caccgcgggc ctcgtgccgg ccctcttcgt ggccctgatg ctccccgcgg gccggcgggt
     5461 ccggcccctt tggggcttcg cggccatagc cggaggctac gccctgggcc tggcgggcag
     5521 catcacgggt tccaaggtcc tgcccctggt cggcatggcg ttttccgcgg gagtggccct
     5581 ggcgggcatc cgcccggtcg aaccttcgaa cacaagggcg tgacaatcgg gcaagggctg
     5641 tcatattgac aatcgacagc ccttgcgtca aactcgcccc cgggacgacg gcgtcccgag
     5701 gaggcgaaca tggccgtact catccgatcc acgggatcct tcgtcccgga caaccgatat
     5761 tccaacgacc agctctccgc gatcgtggac accacggacg aatggatccg ttcccatacg
     5821 ggcatcggcg cccggcatat cgccccggac ggcacgatga ccagcgacct ggccctgggt
     5881 gccgcgaaaa gcgccctgga aaaggcggga atcggcccgg aggaggtcga ttacatcgcc
     5941 gtggccacgg ccagcccgga ctacttcggc tttcccgcca cggcctgcat cgtacaggac
     6001 aagctggggg cctccaaggc ggccgccttc gacgtggtcg cgggatgcac gggcttcatc
     6061 tacgccctgg acacggtttc cgcgatgctg gaggcccgga aggcccggta cggcctggtg
     6121 atcggggcgg agaccctgtc ccggatcgcg gactgggcgg accggtccac ttgcgtcctc
     6181 ttcggggacg gggcgggctg cgccctgctg gaaaactccc aggccgagac ggaccggggc
     6241 ctcaaggcca gcatcctggg atccgacggc gggggagccc tggacctcta cctggcccag
     6301 ggcatgcggg acaagacctt cgaccgcggt gaaccctacc aggatttccc gaaaatcgtc
     6361 atgaacggca agaacgtcta caacttcgcc gtgaaggcca tcaccaagct gatcggggac
     6421 ctcctggaga agagcggcat cccccttccg gagttccgct ggatcgtccc ccaccaggcc
     6481 aacctgcgca tcgtccaggc cgcggcaaag cgcttcggtc ttccggagga acgcttctac
     6541 ctgaacatcc aggagtacgc gaacacttcc gccgcctcgg tgcccatcgc cttgaacgag
     6601 atcgcggaaa aatccctggt gaaagccggg gacccggtca tgctcctggg cttcggcgcg
     6661 gggctgacct tcggcgggag cgtcctgagg tactgaccat cgtccgagac cgcctccggc
     6721 ggccttgccg gggacgggtc cgcgagatac gctaagaatt ccaacgttcg acaggaggaa
     6781 ccatggatac gaaacgccgc gtagtcgtga ccgggatggg ggtcgtgagc cccgtcggca
     6841 acgatcttgc gagcttctgg gacgccgtca agaacggccg ttccggcata ggaccggtga
     6901 cccgcttcga cgcctcccgc ctggaggccc ggatcgcggg ggaggtgaag gacctggacg
     6961 tctccctctg gatggacaag aaggaggccc ggaagatggc cctcttcacc cagtacgccg
     7021 tgtccgcggc ggcccaagcc tgggcccagg ccggcatgga cggggcctcc accccccggg
     7081 accggatcgc catcgtggtg ggcaacggca tcggcggcga ggagatccac tgcgagtccc
     7141 acgccaagat gctggagtcc gggcccgggc ggatcccgcc catgacgatc cccctcatga
     7201 tcatgaacga ggcggcgggg aacatcgccc tgcgcttcca gatcaagggc cccgcctaca
     7261 cccaggccac cgcctgcgcc tccggcacgg acgccctggg ccaggccctg gacatgatcc
     7321 gcgcgggacg ctgcgacgtc gcgatcgcgg gcggcaccga gggggcgatc acggagttcg
     7381 ccatcggggg cttctgccgg atccaggccc tgtccaccaa gcgcaacgac gagccggcca
     7441 aggcctcccg ccccttcgat aaggaccggg acggcttcgt gatcgccgag ggcgccggga
     7501 tgatgatcct ggagtcctgg gagcacgcca aggcccgggg ggccgcgatc ctggcggagt
     7561 tcgcggggta cggggcctcc tgcgactcct accacctgac cgccccggac cccgagggct
     7621 cggggggcgc cctggccatc cgccgggcca tcgaggacgc ggggctcgcc ccctcggaca
     7681 tccagtacta caacgcccac gggacctcca cgccgatcaa cgatcccacg gagaccctca
     7741 tggtcaagaa ggccttcggg gatcacgccg gaaagctcaa gatcagctcc accaagagcg
     7801 tcacgggtca catgatcgcc gccgcgggtg ccgtggaggc catcgtctgc gtgcaggcca
     7861 tccgggaagg attctgccct cccacgatca acctggacga gccggacccc gcctgcgacc
     7921 tggactacgt cccgaacaag ggcgtggccc tcccggtccg ggccgccgcc tccgcctccc
     7981 tgggcttcgg gggacacaac ggctgcgtcg tgttccggag ggccgaatga gcgggaagag
     8041 cgcggaaacc agggcggagg tggaagccct ccttccccac cgggatccct tccgcttcgt
     8101 ggactccgtc cggatcgagg gcgaaaagat cctgggaaag aagctctgga ccgggaacga
     8161 gttcttcttt cccggccact tccccggcca tcccgtggtg cccggggtca tcctcctgga
     8221 aaccatggcc cagtgcgggg gtgtgggggt caaactccag gggatcgtgc ccgcgggaac
     8281 cttcttcttc gccgccatgg acggcatcaa gttccgcaag cccgtgtacc ccggggatac
     8341 cttcgagatc gagatcgtca acctccgggc ctccagccgg gcggtcaagc agcagggcat
     8401 gggctacgtg aacggcgaga aggccgtgga agcctcctgg ctctgtctgg tgggggtgga
     8461 gggaggtccg tcgtgaagcc gatgatccgg aacaacatct gcatgaacgc ccacggcccg
     8521 ggctgcgagg ccctgaccga ggggtggata gcccgggccc tggaaggaaa gccggctatc
     8581 cagtccgccg tcggaacctc ggccaagaag taccggtccg tcctggtcct ggggggctcc
     8641 acaggctacg gcctggcttc ccggatcgtg gccgccttct gcctgggagc cgacacggta
     8701 tccgtctcct tcgagcggga accgagcgac aagcgggcgg gaacaccggg ctggtacaac
     8761 aaccgtgcct tcgaccgggc ggccgtcggg gcgggcctcc ggtcctgggg cctggacgcg
     8821 gacgccttcg cggactccac ccgggaggcg gtgatcgccc tggcccggga gaaggggctc
     8881 atcttcgacc tggtggtcta ctccctggcc tccccggtgc gtacggatcc caagaccggc
     8941 cagatgtacc gctcggtcat caagcccctg gaccggccct acgaggggat caacctggac
     9001 gtcttctccg ggaagatctc ccgggccacg gtccagccgg cctctgccga ggaggcggag
     9061 cacacggtca aggtgatggg cggcgaggac tgggaactct ggatccgcgc cctgcggaac
     9121 acgggggtcc tggccccgga cgccgagacc ctggcctaca cctacatcgg cccggagcac
     9181 tcctggccga tctaccggtc cggcgctctg ggcaaggcca aggcccacct ggaggccacg
     9241 gcccggaccc tggactccct gctcaagccc ggcgggggag ccgcctgggc ggcggtcatg
     9301 aaggccgtgg tgacccgggc ctccgcggtc ataccggtca tccccctcta cgtctccacc
     9361 ctcttcaagg tgatgaagga gaagggagtc cacgaggact gcctggacca gggactgcgg
     9421 cttttccggg accgcctgct caagaaggac gtgcccacgg acccggagcg ccggatccgc
     9481 ctggacgacc tggagatggg cctggaggtc caggccgaga cctcgcggcg catgaagacc
     9541 gtgacggagg agaatgccga gactgtggcc gacatcgagg gcttccggac ggacttcctc
     9601 caggctcacg gttttgctgt acccggcgt
//
LOCUS       DAOTEI010000135         9535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5072_length_9535_cov_4.883017, whole genome shotgun sequence.
ACCESSION   DAOTEI010000135 DAOTEI010000000
VERSION     DAOTEI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_9535_cov_4.883017"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1554)
                     /locus_tag="P5313_12275"
     CDS             complement(109..1554)
                     /locus_tag="P5313_12275"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY81187.1"
                     /translation="METMRPHRTTTLAAKIIRILVIALTVGLGIAALMFGLVLTSSRQ
                     EVTRESLNQEAAMLQTAIENFMLPGEAPIAVQFFREIEGANPKVRISLFRRNGEIAFS
                     DNRTIQEVNANIRREKFAPRSSPALEMQMPADSVHFPKAVSMPPQDVFFRQEDDAAVY
                     IKIYRPLINLPRCTACHGSDHTIRGVIGVQFDVSSFVRSENLALASGGAGFLVLTIIL
                     AAALGSIMNRGVAAPVAAIGKVCERVAQGDFGGVVSVRGNDEIARLGRQVNDMVRGLR
                     ERSELTKYVSAGTLGAIQDSQEPKRVRKTLLFTDVRGFTSYTGSHDPERVVGILNKLL
                     EEQSRIIHEHQGDVDKFVGDEVVAVFSGEDSPARACSAALAIMAAARKGGEAYDGLAL
                     GAGIACGEVIQGMIGSARRADFTVIGASVNIAARLCGLAKPGQILVARDARDAAGTPP
                     GFSFRGPFKTALKGVEGPSVIYLLEPEGDAS"
     gene            1591..2397
                     /locus_tag="P5313_12280"
     CDS             1591..2397
                     /locus_tag="P5313_12280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY81188.1"
                     /translation="MPLKRNGGIDAYSGLVRAYEELFPVHPGTVGYLARAGLANGARV
                     LDVGCASGAHPRAFAAAGYRVVGIDPSEAMIEAARASAARAPTHRKPESGPSSGPKGR
                     PEAPRFETAGMLDLERLFPARTRDAVTCLGNTLPHLRDEAEAEDFLRQARSVLAPGGF
                     LVLQVLNYRRILAERPESLPRLEAGAWVFERSYRYGDGFVEFHTRLIDRSHRTLSEGR
                     TRLLPIFPERIADLAAGRGFRSAALESGWDGEPFDPETSTLAVLRLSIPA"
     assembly_gap    2460..2559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2708..4822)
                     /locus_tag="P5313_12285"
     CDS             complement(2708..4822)
                     /locus_tag="P5313_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY81189.1"
                     /translation="MARKRSLSFRIAFIFSVFIAAAFAATGIITALLVDGRVTELTTE
                     LQGQMTRARGDQMAEFIKKMEATARMVAFRDQVRTADLDRIEELMKALRAQVSEDIDM
                     IFYAGLDGEAVTSLGTRVNVKDRDYFQAVVVGTQRFFISKAVISRATNLPVVLMAHEV
                     LDPAGGRRGLVGIQIDLGKLSQIALSMKSGHTGYGWIADKDGLVIAHPNKDVAMSLVL
                     SETDAKGYKGLSELGRRFLAEESGLGHYSTPDGRDVTTFFVKVPSSPGWALALTQETR
                     EIRQTATELIQVLAVTVVVALVLSILISVLVARSVAKPVDHVRVSVEHVAQGELGKAR
                     LVSDYSRKVLERGDEVGAIAVAINDMIEALVRIATDIRQASGQVSTGSQELSSTAQGL
                     SQGATEQAASVEELSASVEELASTIRQNAENTSQADSLAQRVARNAEDSGKAVAKTSD
                     SMGEIASRISIIEEIARQTNLLALNAAIEAARAGDAGKGFAVVASEVRKLAERSQKAA
                     QEINGLSRDSVSVAQEAGKLLAELVPDIKKTADLILEISAASNEQSSGAEQIAKGITQ
                     MDMVVQQNASASEELAGTSEELASQAELMADTVSFFKLDGASAVRTSRSSEPSVRPSA
                     GPRRLAPPAGSRPAVQGSPSTKPSRTAAKGAKTSAPSPEGGSGQSPKLASAPADRQSR
                     SAATGITLKQPRDAEDSEFEEF"
     gene            complement(5043..6158)
                     /gene="ugpC"
                     /locus_tag="P5313_12290"
     CDS             complement(5043..6158)
                     /gene="ugpC"
                     /locus_tag="P5313_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY81190.1"
                     /translation="MAKVELKGITKVYEGNVTAVEKADIVIEDKEFVVFVGPSGCGKS
                     TTLRMIAGLEDITGGDLFIDGDKVNDIPPKDRNIAMVFQNYALYPHMSVYENMAFGLR
                     IRKTDKAEIDKRVKEAARILDIEKLLERRPKQLSGGQRQRVAVGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRAEIIELHHRLNATMIYVTHDQVEAMTMGDKIVVMKDGKIQQIGS
                     PLYLYNNPVNKFVAGFIGSPPMNFLTVKVVEEGGKILIDEGSFKIQPAGAHLSYLKPY
                     VGKQVSFGIRPEDLPCTEDGAGGIPAKVTVVEPLGAEIHLYAATPTQSMIAKVPPHHL
                     FKIGDSVRFLPVLEKAKYFDRETDEAIIPADRVEKSK"
     gene            complement(6254..7024)
                     /locus_tag="P5313_12295"
     CDS             complement(6254..7024)
                     /locus_tag="P5313_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY81191.1"
                     /translation="MIPLHKLSTLTPSHRLRKAALEFRALEETLGAGGNIDGRYARGL
                     LDLLARDEAVPSEVRSDLGRLALRLEEDPRRACNEARHHMLRLTGKDAADWDFLDASG
                     LSSETRRVLPGFRVYLQDVRSPFNVGSVFRSAEAFGVAEILLSPFCADPGHPRAERSA
                     MGTTRTVPWRRAALADLDGLGPLVALELGGEPLDGFTFPERGILAVGSEELGLSPDLL
                     ARCRYRVSIPMGGAKASLNLGVAFGIAARAWFRGRPGS"
     gene            complement(7172..8323)
                     /locus_tag="P5313_12300"
     CDS             complement(7172..8323)
                     /locus_tag="P5313_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRY81192.1"
                     /translation="MADFRFRVPAETYMGPDTLLKLPSLLASYGSDRVLVVADPMLHE
                     ARAVDRVLALLEDRGSKAILFDEIPDGATTASAEDILRLARGARANGVVGLGGVRVLS
                     AARAVAASAAGTASLDDWIDGTPPTCRPLPYISVPTTYRDPFLLTDECALTDARGRRA
                     RVLAASPGTTRAVILDPSMSTGLFPKAAAACMLDVLLQAIEGFVSARANFYSDVLLER
                     AVALAASCVDAFQTRPDDPAVRADALRASFLTGAGLASSSLGLGTAVVLALNARFQVP
                     KASLAAVVLPYMLEWASKSRLEKVAALAPLLGEDVSELTTAESAVKAVESVRTRLGLL
                     KIPSRLKDFDLELERLVETAEHARTLDMTNHLPRTVSTDDVFDMMKTAY"
     gene            complement(8405..8686)
                     /locus_tag="P5313_12305"
     CDS             complement(8405..8686)
                     /locus_tag="P5313_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRY81193.1"
                     /translation="MTIDRLHPIDPIQNPRKSDGPHRADKGSKGDSIAVSSEAMEKAE
                     LFRVIEMVKAAPDVRADRIAELKAKIDDPAYLDETLLAATADRILDQLL"
     gene            complement(8740..9420)
                     /gene="rsmI"
                     /locus_tag="P5313_12310"
     CDS             complement(8740..9420)
                     /gene="rsmI"
                     /locus_tag="P5313_12310"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRY81194.1"
                     /translation="MATLYMVATPIGNLQDITLRALEVLKSVDTVACEDTRHTLKLLT
                     HFGIRKPLVACYAYEEEKGARRILGLLDSGKDVAYCSDAGTPALSDPGARAAALAREG
                     GHDVVPVPGPSAFACLLSASGLPVKSVLFEGFLSPKPGRRRSRLAELLARGEAFVVYE
                     SPHRILKLLLDLADLDPECRVFAGREMTKAFEEYMAGTPGEVCSALESREKVQGEFSV
                     LVYGGKNA"
BASE COUNT         1494 a         3099 c         3252 g         1590 t
ORIGIN      
        1 acttcggggt cgcgggcccg caggccgaaa ggaccgccag gaccgccagg atcgttgcgc
       61 aggcgggcag gatcgccgcg gccgcggcag gaacgtgtcg ggcagtcctc atgaagcgtc
      121 tccttcgggt tcgagaaggt agatcacgct ggggccctcg acccccttca gggccgtctt
      181 gaagggaccc cggaacgaga accccggcgg cgtgccggcc gcgtcccggg cgtcccgggc
      241 caccaggatc tgtcccggct tggccaggcc gcagagccgg gcggctatgt tgaccgaggc
      301 tccgatgacg gtgaagtccg cccggcgggc cgagccgatc atgccctgga tcacctctcc
      361 gcaggcgatg cccgccccca gggccaggcc gtcgtaggct tctccgcctt tccgggccgc
      421 ggccatgatg gcaagggcgg cggagcaggc ccgggcgggg ctgtcctccc ccgagaagac
      481 cgccaccacc tcgtccccca cgaacttgtc cacgtccccc tggtgctcgt ggatgatccg
      541 ggactgctcc tccaggagct tgttgaggat tccgaccacc cgttccgggt cgtgggaacc
      601 cgtataagag gtgaagcccc ggacgtccgt gaagagcagg gtcttccgga cccgcttggg
      661 ctcctgggaa tcctggatcg ccccgagggt gcccgcggag acgtacttgg tcagttcgga
      721 gcgctcccgg agtccccgga ccatgtcgtt cacctgccgt cccagccggg cgatctcgtc
      781 gtttccccgg acgctgacga ctcctccgaa gtccccctgg gccacccgtt cgcagacctt
      841 tccgatggcc gcgacggggg cggctactcc ccggttcatg atgctcccca gcgccgcggc
      901 caggatgatg gtcaggacca ggaagcccgc gccgccggag gccagggcga gattctcgga
      961 ccggacgaag gaactgacgt cgaactggac cccgatgact ccgcggatgg tgtggtcgga
     1021 gccgtggcag gccgtacacc ggggaaggtt gatgaggggc cggtagatct tgatgtagac
     1081 cgccgcatcg tcctcctggc ggaagaacac gtcctggggg ggcatggaca ccgccttggg
     1141 aaagtggacg ctgtccgcgg gcatctgcat ctccagggcc ggggacgatc ggggggcgaa
     1201 cttttccctg cggatgttcg cgttgacctc ctggatggtc cggttgtcgg agaaggcaat
     1261 ctccccgttc cgccggaaca gggagatccg gaccttgggg ttggcgccct cgatctcccg
     1321 gaagaactgc accgcgatcg gggcctcccc gggaagcatg aagttctcga tggcggtctg
     1381 gagcatggcc gcttcctggt tcaggctctc ccgggtgacc tcctgccggg aactcgtgag
     1441 caccaggccg aacatcaggg ccgctatccc gaggccgacc gtcagggcaa tcacgaggat
     1501 ccggatgatc ttggccgcca gggtcgtcgt gcggtgaggg cgcatcgttt ccatgtatac
     1561 agcctagaga cctggggtct agaatgcaat atgcccttga aaaggaacgg cgggatcgac
     1621 gcatattcgg ggctcgtccg agcctatgag gaactcttcc cggtccaccc cggcaccgtg
     1681 ggctatctgg cccgggcggg cctggctaac ggtgcccggg tcctggacgt cggctgcgcc
     1741 tccggggccc acccccgggc cttcgccgcc gcggggtaca gggtcgtggg gatcgacccc
     1801 tcggaggcca tgatcgaggc cgcacgggct tcggccgcac gggcgccgac gcaccgcaaa
     1861 ccggagtccg gtccgtcttc cggcccgaag ggccgtccgg aagccccgcg gttcgagacc
     1921 gcaggcatgc tcgacctgga gcggcttttc ccggcgagga ctagggacgc ggtcacctgc
     1981 ctggggaaca ccctccccca cctccgggac gaggccgagg cggaggactt cctgcggcag
     2041 gcccggtccg tcctggcccc ggggggcttc ctggtgcttc aggtcctgaa ctaccgccgg
     2101 atcctggccg aacggccgga aagcctgccc cgtctggagg ccggggcctg ggtcttcgag
     2161 cggtcctatc ggtacgggga cggcttcgtc gaattccaca cccggctcat agaccggagc
     2221 catcgtaccc tctccgaagg acggacccgc cttttgccca tcttcccgga gaggatcgcg
     2281 gacctggcgg cgggccgggg cttccggagc gccgccctgg agtccggctg ggacggggag
     2341 cccttcgacc cggagacctc aaccctggcg gttctgcggc ttagcatccc ggcttgaatg
     2401 ccgaaggcgc ggattatccg gggcagccgg tcgcgctgcc gcgcgaccga tcaaccgtgn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgccgcaggc gcggattatc
     2581 cggggcagcc ggtcgcgctg ccgcgcgacc gatcaaccgt ggcgcgccgc aggcgcggat
     2641 tatccggggc agccggtcgc gctgccgcgc gaccgatcta ccgtggcgcg ccgcaggcgc
     2701 gccacggtta gaactcctca aattccgaat cctccgcgtc ccggggctgt ttcagggtga
     2761 tgcccgtggc ggctgatcgg ctttgccgat cagccggcgc cgaagcaagc ttcggcgatt
     2821 gtccggatcc gccttccgga gacggcgcgg aagtcttcgc gccctttgcc gcggtccggg
     2881 acggtttggt agaagggctt ccctggaccg ccggcctgct tcccgcggga ggggcgagcc
     2941 tccggggacc cgccgatggg cggacggagg gctccgagga ccgggaggtc cgaaccgccg
     3001 acgcgccgtc cagcttgaag aaggacacgg tgtccgccat gagctccgcc tgggaggcga
     3061 gctcctcgct cgtccccgcg agctcctcgc tggccgaggc gttctgctgg accaccatgt
     3121 ccatctgggt gatgcccttg gcgatctgct ccgccccgct ggactgctcg ttggaggcgg
     3181 cgctgatctc caggatcagg tccgcggtct tcttgatgtc cgggaccagt tccgccagga
     3241 gctttccggc ctcctgggcc accgacacgg aatcccggga aaggccgttg atctcctggg
     3301 cggccttctg gctccgctcc gcgagcttgc ggacctcgct ggcgaccacg gcgaaaccct
     3361 ttccggcgtc cccggcccgg gccgcctcga tggcggcgtt caaggccagg aggttggtct
     3421 gccgggcgat ctcctcgatg atggagatcc gggaggcgat ctcccccatg ctgtccgagg
     3481 tcttggccac ggccttgccg gagtcctcgg cgttccgcgc cacccgctgg gccagggagt
     3541 ccgcctggga ggtgttctcc gcgttttgcc gtatcgtgga ggcaagctcc tccacggacg
     3601 ccgagagctc ctccacgctg gcggcctgct cggtggcgcc ctgggaaagg ccctgggccg
     3661 tgctggagag ctcctgggaa cccgtggaaa cctggccgga ggcctggcgg atgtccgtgg
     3721 cgatccggac cagggcctcg atcatgtcgt tgatggccac cgcgatggcc ccgacctcgt
     3781 ccccccgctc caggaccttg cgggaatagt ccgagaccag gcgggccttg ccgagctcgc
     3841 cctgggccac gtgctccacc gagacgcgga catggtccac gggcttggcc acgctgcggg
     3901 ccacaagtac ggagatcagg atggacagga ccagggccac gacgactgtc acggccagga
     3961 cctggatgag ctccgtggct gtctggcgga tctcccgggt ctcctgggtc agggccaagg
     4021 cccagcccgg actgctcggg accttcacga agaaggtggt cacgtcccgc ccgtccgggg
     4081 tgctgtagtg acccaggccg gattcctcgg ccaggaagcg gcgcccgagc tccgacagcc
     4141 ccttgtaccc cttggcgtcc gtctccgaga gcacgaggct catggccacg tccttgttgg
     4201 ggtgggcgat caccaggccg tccttgtccg cgatccagcc gtacccggtg tgcccggact
     4261 tcatggacag ggcgatctgg gacagcttgc ccaggtcgat ctggatgcct accaggcctc
     4321 gccgtccccc cgcgggatcc aggacctcgt gggccatcag gaccaccggg aggttcgtgg
     4381 cacgggagat cacggccttg gagatgaaga accgctgggt tccgaccacc acggcctgga
     4441 aatagtcccg gtccttgacg ttcacccggg tccccaggga ggtgaccgcc tccccgtcca
     4501 gacccgcgta gaagatcatg tcgatatcct cggagacctg ggcccttaag gccttcatga
     4561 gctcctcgat gcggtctagg tccgcggtcc ggacctggtc ccggaaggcc accatgcggg
     4621 cggtggcctc catcttcttg atgaattccg ccatctggtc gccccgggcc cgggtcatct
     4681 gcccctggag ctccgtcgtg agttccgtga cacgaccgtc caccaggagg gcggttatga
     4741 ttcccgtggc cgcgaaggcc gcggcgatga acacgctgaa gatgaaggcg atgcggaagg
     4801 atagggagcg tttgcgggcc attggcacct ccaaaaaagt agagccgtac acgttcgggt
     4861 ttcaccctgg ctttttgaaa cggtgctgaa tgggagggct cggctcctga catatactcc
     4921 gaatatgtca gacatcaccg tcctcaattg aattttagtg attaccgggg aaaaagcaaa
     4981 caaaaaaagg ggccggcctg agccggcccc cgaggggtcg tcgcaacgga tcgggcggcg
     5041 ccctacttcg acttctccac ccggtccgcg gggatgatgg cctcgtcggt ctcccgatcg
     5101 aagtacttgg ccttctccag gaccggcagg aaacgtacgg aatctccgat cttgaagagg
     5161 tggtggggcg ggaccttggc gatcatggac tgggtcgggg tcgcggcgta gaggtggatc
     5221 tccgctccca ggggctccac cacggtcacc tttgcgggga tgcccccggc tccgtcctcg
     5281 gtgcagggca gatcctcggg ccggatgccg aaggagacct gcttgccgac gtagggcttc
     5341 aggtaggaca ggtgggcccc ggcgggctgg atcttgaagg agccctcgtc gatcaggatc
     5401 ttgccgccct cctcgacgac cttgacggtc aggaagttca tgggcgggga tccgatgaat
     5461 ccggccacga acttgttgac cgggttgttg tacaggtaca ggggggatcc gatctgctgg
     5521 atcttcccgt ccttcatgac cacgatcttg tcccccatgg tcatggcctc cacctggtcg
     5581 tgggtcacgt agatcatggt ggcgttcagg cggtggtgga gctcgatgat ctccgcgcgc
     5641 atctggaccc ggagcttggc gtccaggttg gacaaaggct cgtcgaagag gaagaccttg
     5701 ggattccgga cgatggcccg cccgacggcc acgcgctggc gctggcctcc ggacagctgc
     5761 ttgggccggc gctccaggag cttctcgatg tccaggatgc gggcagcctc cttgacccgc
     5821 ttgtcgatct ccgccttgtc ggtcttgcgg atgcgcaggc cgaaggccat gttctcgtac
     5881 acgctcatgt gggggtacag ggcgtagttc tggaacacca tggcgatgtt ccggtccttg
     5941 ggggggatgt cgttgacctt gtccccgtcg atgaagaggt cgccccccgt gatgtcctcc
     6001 aggcccgcga tcatgcgcag ggtggtggac ttgccgcagc cggacggccc gacgaagacg
     6061 acgaactcct tgtcctcgat gacgatgtcc gccttctcca cggcggtcac gttgccttcg
     6121 tagaccttcg tgattccctt gagctctacc tttgccatac ttcctccgga tcggggtatc
     6181 ttatggaagg cattgtacgg cggatttccg ggtatgtcaa aaaaaagagg gggcggccgt
     6241 ccaaaaagcc ggcctagctt cctggacggc cgcggaacca tgcccgggcg gcgatcccga
     6301 aggccacccc caggttgagg ctggccttgg ctccccccat ggggatcgac acccggtatc
     6361 ggcaccgggc caggaggtcc ggggaaagcc cgagctcctc ggaacccacg gccaggatcc
     6421 cccgttccgg gaaggtaaag ccatccaggg gctccccgcc cagctccagg gccaccaggg
     6481 ggcccaggcc gtccaggtcc gcgagggccg cgcggcgcca gggtacggtc cgggtggtgc
     6541 ccatggccga cctctcggcc cggggatggc cgggatcggc gcagaagggg gacaggagga
     6601 tctccgccac cccgaaggcc tcggcgctcc ggaacacgga acccacgttg aagggactcc
     6661 gcacgtcctg gaggtagacc cggaagcccg gcaggacccg gcgggtctcc gaggaaagcc
     6721 ccgaggcgtc cagaaagtcc cagtccgcgg cgtccttccc ggtcaatcgg agcatgtggt
     6781 gccgggcctc gttgcaggcg cgccgggggt cctcctcgag ccgcagggcc aggcggccca
     6841 ggtccgagcg tacctcggag gggacggcct cgtcccgggc aagcagatct aaaaggcccc
     6901 gggcgtagcg cccgtcgatg ttccccccgg cgcccagggt ctcctccagg gcccggaact
     6961 ccagggcggc cttccgcagc cggtgggagg gggtgagggt ggagagcttg tggaggggga
     7021 tcatgggaga aggtgaacag tgaatagtga atagtgaata ggtgactcga tcgtcctggt
     7081 tgatcttctc accatagcaa gaagtctttc tccatgcctt aaatcagttc cactattcac
     7141 caatttaccc attcaccaat tcacgttccc atcaatacgc cgtcttcatc atgtcgaaca
     7201 cgtcgtcggt ggacacggtg cggggcaggt ggttggtcat gtccagggtg cgggcgtgct
     7261 ccgcggtctc caccaggcgc tccagttcca ggtcgaagtc cttgaggcgg ctggggatct
     7321 tgaggagccc caatcgggtg cgcacggact ccacggcctt gaccgcggac tcggcggtgg
     7381 taagttcgga cacgtcctct cccaggaggg gggccagggc ggccaccttc tccagccggc
     7441 tcttggaggc ccattccagc atgtagggca ggaccaccgc ggccaggctg gccttgggca
     7501 cctggaaccg ggcgttcagg gccaggacca ccgcggtccc caggcccagg gagctggagg
     7561 ccaggccggc cccggtcagg aaggaggccc ggagggcgtc cgcccggacc gccggatcgt
     7621 ccggccgggt ctggaaggcg tccacgcagg aggcggccag ggccaccgcc cgctccagga
     7681 gcacgtcgga gtagaagttg gcccgggcgg acacgaagcc ctcgatggcc tggagcagga
     7741 cgtccagcat gcaggccgcc gcggccttgg ggaagagccc cgtggacatc gaggggtcga
     7801 ggatcacggc ccgggtcgtc ccgggagagg cggccaggac ccgggcccgc cggccccggg
     7861 cgtccgtcag ggcgcactcg tcggtgagta ggaagggatc ccggtaggtg gtgggtacgg
     7921 agatgtacgg aagcgggcgg caggtcgggg gagttccgtc gatccagtcg tccagggagg
     7981 ccgtccctgc ggccgaggcg gccaccgccc gggccgcgga caggactcgg accccgccca
     8041 ggcccacgac cccgttggcc cgggcccccc gggccaggcg gaggatgtcc tccgcggagg
     8101 cggtggtggc gccgtccggg atttcgtcga acaggatggc cttggagccc cgatcctcca
     8161 ggagggccag gacccggtcc accgcccggg cctcgtggag catgggatcc gcgaccacca
     8221 ggacgcggtc cgagccgtag gacgcaagca ggctgggaag cttgagtagg gtgtccggcc
     8281 ccatgtaggt ttccgcgggg acacggaatc ggaagtctgc catctcgctc gcctccggag
     8341 gggcatgtac gtaaaaaagg cggaagactc gcttccgcct gtgtttctcg ccgtgaaccg
     8401 gaccttacag gagctggtcg agtatccggt ccgccgtcgc ggccaggagg gtctcgtcca
     8461 ggtaggcggg gtcgtcgatc ttggccttga gctccgcgat ccggtccgca cggacgtccg
     8521 gcgcggcctt gaccatctcg atgacgcgga agagttccgc cttctccatc gcctcggagg
     8581 atacggcgat ggaatcgccc ttggagccct tgtccgcccg atgggggccg tcggacttcc
     8641 gggggttctg tatcgggtcg ataggatgta gtcggtcgat cgtcatcggg aaacccctgc
     8701 ctttctcatc atgagtatcg gcatcgatcc acgaatagtt tatgcgtttt taccgccgta
     8761 tacaagtacc gagaactccc cctgcacctt ttcccgggat tccagggccg aacagacctc
     8821 cccgggcgtc ccggccatgt actcctcgaa ggccttggtc atctcccggc cggcgaagac
     8881 ccggcactcc ggatccaggt cggccaggtc caaaaggagt ttcaggatcc ggtgggggga
     8941 ctcgtagacc acgaaggcct ccccccgggc caggagctcc gccagccggg agcgccgtcg
     9001 gcccggcttc ggggagagga agccctcgaa gaggacggat ttcacgggaa gccccgaggc
     9061 ggacaggagg caggcgaagg ccgagggccc cggtacgggg actacgtcgt gccccccctc
     9121 ccgggccagg gcggccgccc gggccccggg atcgctcagg gcgggagtcc ccgcgtcgga
     9181 gcagtaggcc acgtccttgc cggagtccag gagccccagg atcctgcggg cccccttctc
     9241 ctcctcgtag gcgtagcacg cgaccagggg cttgcgtatc ccgaaatggg tcaggagctt
     9301 cagggtgtgg cgggtgtcct cgcaggccac ggtatccacg gacttgagga cctccagggc
     9361 ccggagggtg atgtcctgga ggttaccgat gggggtggcc accatgtaca gcgtcgccat
     9421 ggggggagta tagcggttgg aaaggtgaat agtgaatagt gaataggtga atagaggggc
     9481 cacccattca cccattcacc cattcaccca ttcacccatt cacccattca cccat
//
LOCUS       DAOTEI010000136         9512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5094_length_9512_cov_4.940890, whole genome shotgun sequence.
ACCESSION   DAOTEI010000136 DAOTEI010000000
VERSION     DAOTEI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5094_length_9512_cov_4.940890"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="P5313_12315"
     CDS             <1..702
                     /locus_tag="P5313_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRY81195.1"
                     /translation="LGPELFDYGYGPFRWVCLSGKPDDLRKTDRAAMDCIDPNRRGQD
                     RDNWAWIRDAEKNRLVVGTQARILYQDAMGRTKIALRFNEMVRNGETGPIMLGRDHHD
                     CGGTDSPFRETSNIKDGSNVMADMATQIWGGNAARGMSLVALHNGGGVGIGKAINGGF
                     GLVLDGSERVDRIIRMAMPWDVMGGVARRAWARNGHAVSTCTEYNDVYAEYGHVTLPY
                     VADDELLRKAVGKKA"
     gene            699..1742
                     /locus_tag="P5313_12320"
     CDS             699..1742
                     /locus_tag="P5313_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRY81196.1"
                     /translation="MKAKSAGSARKHRPSAIPGGSWTWKPPEPWGGRVDDPEDPAALR
                     WHQVVRPWDGMPIGAGSGTRRIWFIGCPTDIGVSRNKGRPGAARGSGSLRRALANLPV
                     RFGDALELIDAGDLEPRDGDLEGLMEGLELLVGAVVASGGFPVVLGGGHETAYGHHRG
                     LVRGLAALSLPAPRIISFDAHFDLRPEGPPGAPLHSGNMFRLLAREAGERGEPLEYDV
                     IGIQRSANTLSLFREADGLGVRYLPAREAGSPEAILGFLRTGRIGDRPLHVTLCTDVI
                     SAAHAPAVSAPQPFGLDPNVVLEGLRHLARSGRAAGLDVAEVSPRYEGDDGTAHLAAV
                     LLFAWVNAMAGSD"
     gene            complement(1822..3198)
                     /locus_tag="P5313_12325"
     CDS             complement(1822..3198)
                     /locus_tag="P5313_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HRY81197.1"
                     /translation="MSERKSYGASAALKFIGLSLFGLFMFFVPFDLMIKEIGYFRMVS
                     GAQAPINTIAVDHIVGMIRKIPSFDVVWGTAIVLIGIVYPLATGSWKKDLTSKVFMVF
                     KVLALLPLSMVLFKWQLVPNMPKGADLSFIWLRVVVPVTTLVPVGAVFLAFITTYGLM
                     EFFGVFLRPVMRPIWKTPGKSAVDAVASFVGSYSVGLLITDRMYQTHRYSDKEAAIIA
                     TGFSTVSATFMVIVANTTGLGGPKFVAYFWSTLVVTFLVTAITARLFPIARKKDTGFE
                     GKAPETEPKFPASEIPRESWILACEAAERSGNVLKQMAGNIAAGVRLSIAIGPTLLSI
                     GYFGLVLAHFGIFNVIGYIFLPFTWLAGLIFPALGSPMVLAGALASSLAEMFIPVGVI
                     AGGSALVKAVVAIVCVSEIIFFSASIPCILSTKIPLSIGELLVVWFERVVLSLFLALP
                     FALILVGG"
     gene            complement(3354..3806)
                     /locus_tag="P5313_12330"
     CDS             complement(3354..3806)
                     /locus_tag="P5313_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY81198.1"
                     /translation="MEGTRSLAALTVEEIRELWSRTYNREGKPDWSHIFPYYHEDVVF
                     RDSIQEIRGIEEFKALCGRLTDRCQQLRMKVPSVVHGGDCVFLQWEMTMIFKGYPSST
                     IYGATRLGLSGDGRIITQRDYYDLWGDIFDNIPWFAKPYRRFMKKKFG"
     gene            3883..4866
                     /locus_tag="P5313_12335"
     CDS             3883..4866
                     /locus_tag="P5313_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY81199.1"
                     /translation="MTSRTARYVREYTWSNVFAMIRNNGLEPKICGDDCKGKLVVITG
                     ATSGIGRAAARKFASRGADLLCVNRNPEKSEALRREIEGEFGTRCEYRIADLSRLEDI
                     RRTAEELSRPGSPIDVLVHNAGVYLTRRELTSDGLETTFVVHYLSSFILNYLLMDTLK
                     ARGKARILMVGSEGYRFAAWGLRLDDLNWERRKYSGLASYGSAKLAQLLSMIVFDERL
                     RGTGVTINTMHPGAVKTETGQENGPAYRWFKRNFFDKTLKSPELSAEALYYLGAAKEL
                     EGVSGKFFNLTTEEEPAPPARDREAAYELWRETLKMTGLEDAESVPGHDPK"
     gene            complement(4888..6138)
                     /gene="hutI"
                     /locus_tag="P5313_12340"
     CDS             complement(4888..6138)
                     /gene="hutI"
                     /locus_tag="P5313_12340"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRY81200.1"
                     /translation="MSTADLFVRDIRELATPTGGKAQRGPEMAEVRSVPDAAVLIRDG
                     RIAWAGPASEAPAIPEDVPVLSAGGGTVVPGFVDSHTHFVFAGDRSEEFLWRAGGLPY
                     MEIHRRGGGIRRSMDATRAASLDELVAIGRRRLGTMVSLGVTTVEGKSGYGMDLATEL
                     RQLEAMDILGREQPVRIVPTFLGPHSTPPEYAGRTSEYMDYVIRDMLPAVKARGLARF
                     CDVFCEKGVFELADSRRYLEAAKAAGFGLKVHADEIERIGGAALAAELGAASADHLLK
                     CVPSDYEALARSSTVATCLPLTAFVLREPYADARGMIDSGCAVALASDLNPGSCYSQS
                     IPLIFALAVLYMRLSVDEALCALTLNGAAALGLAGEVGSIEPGKRGDLVILDAPSRAF
                     LAYHSGMNLVKTTVIGGRLEYERP"
     gene            complement(6147..>9512)
                     /locus_tag="P5313_12345"
     CDS             complement(6147..>9512)
                     /locus_tag="P5313_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81201.1"
                     /translation="YSREGTAFVELARELIENYREKDRLLTGYLPPADQRIQAFLSDY
                     LADTGLPEIPRLPSNTLTLDRYGMARELSLPPEASTYSSPTLTSYRIRNGILHNPAND
                     RRTTEGVFHVADGGLPVPPDKKAVPKAVFGRILAAAVTPPQEMLTLPFTAQEPKPGRT
                     FLSLLLRPVVQPEVPGICPERSMEIRFFAPGSLAANLDFVESIFGNSGDPYIAENDSA
                     LDPLHWTGHTGCILLATHLVGLKKRDLGLPRWDDASERQRRDGMAWKDPEELYNEGKP
                     FKLCARNERGVIFTIIADNYFGYSKKEVKTQISFSSNLLGMTEEEHAGGALVFPTYNL
                     GTRFVPDANLKARGHNFDEVVSLLKDHLEEHPDGFARDRTFPNIVYLPENAQISLEDQ
                     QARWTRDGKEYSLRILPNETYIHPTGYKIGMNRHAGSGAWRLVGTLATGLLCHKPCTV
                     SGGGKSEISKSIWDAIEYFPFIIGDYEADMAQVRDILERDYSGRFRATKEKHRPDERR
                     VLSSQRSLGSVIKLLSPSPLYTDEYNDWLKTIPERIKALVFLIKRFYVPDWGEDWAKH
                     FTVDTVNGTAGNILKFEGRPVLGSYLRVGYSQDGRRRIFKLRQDYLPAEKVQWEDDIT
                     ASIVLPKASLPELEKASPGISVKFCRNAEARFFQRPDDAVIRGYDKQAEKDMARPDNF
                     ISNFEPLSRSKAHAMIEKTIEFSAYTEPMRRFIEDAEKDDGFDWFVASDRPRLVDGKP
                     SKNPRYLQLDPNHIEPCKRYLADLGHRLFYRIPEDKPVLHPVGAALPGRRNNPADPAG
                     KIRPLAVYGPIHYQELPELFMDFVCSLTGKSPSTTGAGSEGALTKGPFNALVPTTDLN
                     AALLSYILTGYGGFSSAAGHIGGKYKVNHDVSLLVPELWSRMDPEEKDPAFLIANGYL
                     EKIEDFDYQGRRIPASRLGYRITSLFATTFLGRIFDTPSSVFPEDFLKPELQDLAEFA
                     DGVENIAEAQAKVARDYLADGSVEAAIPPLAAILHVMAEGTWNGKTIDDPELRRLFDR
                     DYVLASDWYKERLDRYRDKQVTFLERSIVEIRKAKADQRFTDPAFAPRLDKILKAAEK
                     ELRVVLDPAFVEGLSGTLGADPLFTGRKPVPRK"
BASE COUNT         1625 a         2976 c         3364 g         1547 t
ORIGIN      
        1 ctggggcccg agctcttcga ctacgggtac ggtcccttcc gctgggtctg cctgtccggc
       61 aagcccgacg acttgcgcaa gaccgaccgg gcggccatgg actgcatcga cccgaaccgc
      121 cggggccagg atcgggacaa ctgggcatgg atccgggatg ccgagaagaa ccgcctggtc
      181 gtcgggaccc aggcacggat cctctaccag gacgccatgg gccgcacgaa gatcgccctg
      241 aggttcaacg agatggtccg gaacggcgag acggggccga tcatgctcgg ccgtgaccac
      301 cacgactgtg gcggaacgga cagccccttc cgggagacct ccaacatcaa ggacggctcc
      361 aacgtcatgg cggacatggc cacccaaatc tggggaggca acgcggcccg gggcatgagc
      421 ctggtggccc tgcacaacgg aggaggggtc ggtatcggca aggctatcaa cgggggcttc
      481 ggcctggtgc tggacgggtc cgagcgggtg gaccggatca tccggatggc tatgccctgg
      541 gacgtcatgg gcggggtggc gcgccgtgcc tgggcccgga acggccacgc ggtttcgacc
      601 tgcaccgagt acaacgatgt ctacgccgaa tacggacacg tgaccctgcc gtacgtggcg
      661 gacgacgaac tcctgcggaa ggccgtaggg aagaaggctt gaaggcgaag agcgccggga
      721 gcgcccgcaa acaccggccc tccgcgatac cgggcgggtc ctggacgtgg aagcccccgg
      781 agccctgggg cggccgggtg gacgacccgg aggatccagc cgccctgcgc tggcaccagg
      841 tcgtccggcc gtgggatggt atgcccatcg gcgccggatc gggcacccgg aggatttggt
      901 tcataggatg cccgacggac atcggggtct cccggaacaa gggtcgtccc ggggcggccc
      961 ggggttccgg gagcctgcgg agggccctgg ccaacttgcc cgtgcgcttc ggggacgccc
     1021 tagagctgat cgacgccggg gacctggagc cccgggacgg ggacctcgag gggctcatgg
     1081 agggtctcga gctcctggtc ggcgccgtcg tcgcctccgg gggcttcccc gtcgtgctcg
     1141 gcgggggcca cgagacggcc tacggccatc accggggact ggtccggggg ctggccgccc
     1201 tgtccctccc ggcccctagg atcataagtt tcgacgccca tttcgacctg cgtccggaag
     1261 gtccccccgg ggcacccctg cactcgggga acatgttcag gcttcttgcc cgggaggccg
     1321 gggagcgggg cgagcccctc gagtacgacg tgatcgggat ccagcgctcc gccaacacct
     1381 tgagcctgtt ccgggaggcg gacgggctgg gggtccggta cctgccggcc cgggaggcgg
     1441 ggagccccga agcgatcctg gggttcctgc ggaccgggcg catcggagac cggcccctcc
     1501 acgtgaccct ctgcaccgac gtgatttccg cggcccacgc ccccgcggtc tccgcccccc
     1561 agcccttcgg actggacccg aacgtggtgc tggaaggcct gcgccacttg gcccggagcg
     1621 gccgagcggc gggcttggat gtcgccgaag tctctccccg gtacgagggg gacgacggca
     1681 cggcccattt ggcggcggtg ctcctcttcg cctgggtgaa cgcgatggcg gggtcggact
     1741 gatccggctt tcgacgcttc gtataaaaag gcccggctgt acgccgggcc tttccgttcc
     1801 caggggacac cgtcgccgac gtcagccccc gaccaggatc agtgcgaagg gcagggcaag
     1861 gaagagggag aggaccaccc gctcgaacca cacgaccagg agttctccga tggacagggg
     1921 aatcttcgtg gacaggatgc aggggatgga cgcggagaag aagatgatct ccgatacgca
     1981 gacgatggcc accacggcct tgacaagggc agaccccccg gcgatcacgc ccacggggat
     2041 gaacatctcc gccagggagg acgccagggc ccccgccagg accatggggc tgcccaaggc
     2101 cgggaagatg aggccggcca gccaggtgaa gggcaggaat atgtagccga tcacgttgaa
     2161 gatcccgaag tgggccagca ccaggccgaa gtatccgatg gacaggaggg tgggaccgat
     2221 ggcgatggac aggcggactc ccgccgcaat gtttccggcc atctgcttga ggacgttgcc
     2281 ggagcgctcc gcggcctcgc aggccaggat ccaggattcc cgggggatct cggaggccgg
     2341 gaatttcggt tccgtttccg gagccttgcc ctcgaacccc gtgtccttct tgcgggcgat
     2401 cgggaagagc cgggccgtga tcgcggtcac caggaaggtt accaccaggg tggaccaaaa
     2461 ataggccacg aacttaggac cgcccaggcc cgtcgtgttg gccacgatga ccatgaaggt
     2521 ggcggaaacc gtggagaagc ccgtggcgat gatcgccgct tccttatccg agtaccgatg
     2581 agtctggtac atgcggtccg tgatcaggag gcccacggag tagcttccga cgaaggaggc
     2641 caccgcgtcc accgcggact tgcccggggt cttccagatc gggcgcatga ccggccgcag
     2701 gaataccccg aagaactcca tgagaccgta ggtggtgatg aaggccagga acacagctcc
     2761 aaccggaacc agggtcgtga cgggcaccac gacgcggagc cagatgaagg agaggtccgc
     2821 gcccttgggc atgttcggca cgagttgcca cttgaacagg accatggaca ggggcagcag
     2881 ggccaggacc ttgaagacca tgaagacctt ggaagtcagg tccttcttcc agctgcccgt
     2941 ggccagggga tacacgatgc cgatcaggac gatggccgtt ccccacacga cgtcgaagct
     3001 ggggatcttc cggatcatcc cgacgatgtg gtccacggcg atggtattga tcggagcctg
     3061 ggcgccggag accatccgga agtagccgat ctccttgatc atcaggtcga agggcacgaa
     3121 gaacatgaac aggccgaaca ggctcaatcc gatgaacttc agggccgccg acgctccata
     3181 cgatttccgt tcagacaagg ggcacctccc gattggatcg gccgttccgc acgagtcgat
     3241 cggggccggc cggtttccgg atagtataga aggggctttc aactcctgta aaattagaaa
     3301 aaccgatagc aaagaagcag ggatgatacg acccggctaa gagtcctcgt ctcctacccg
     3361 aacttctttt tcatgaaccg ccgatagggc ttggcgaacc aggggatgtt gtcgaagatg
     3421 tcgccccaca ggtcgtagta gtcccgctgg gtgatgatcc gtccgtcccc ggagagcccc
     3481 agccgggtgg caccgtagat ggtcgagctg gggtacccct tgaagatcat ggtcatctcc
     3541 cactgcagga agacgcagtc cccgccgtgg accacgctgg gcaccttcat gcggagctgc
     3601 tggcagcggt ccgtgaggcg accgcagagg gccttgaact cctcgattcc ccggatctcc
     3661 tggatgctgt cccggaagac gacatcctcg tggtagtacg ggaagatgtg ggaccagtcg
     3721 ggcttgccct cccggttgta ggtccgggac cagagttccc ggatctcttc gacggtcagg
     3781 gcggccaggg atcgcgtgcc ttccatgagc ttgtccttgt atgataaaat caagaatgta
     3841 tcgcaggata caacagccgg aaccggagcg caaggacatc ccatgacaag cagaaccgcc
     3901 cgatacgtcc gggagtacac gtggtccaac gtattcgcca tgatccggaa caacggcctt
     3961 gagccgaaga tctgtggcga cgactgcaag ggaaaactcg tggtcatcac cggagcgacc
     4021 tcggggatcg gccgcgccgc cgcccggaag ttcgcgtccc gaggcgcgga cctgctgtgc
     4081 gtcaaccgga acccggaaaa gtcggaagcc ctgcgacggg agatcgaggg cgagttcggg
     4141 acgcgctgcg agtacaggat cgccgacctc agccgccttg aggacatccg ccgtaccgcc
     4201 gaggagctgt cgaggccggg atctcccatc gacgtcctcg tacacaacgc gggggtgtac
     4261 ctgacccggc gggaactcac gtccgacggc ctggagacca cgttcgtggt ccattacctt
     4321 tcgtccttca tcctgaacta tctgttgatg gacacgctca aggcccgagg caaggcccgg
     4381 atcctcatgg tcggctccga gggataccgc ttcgccgcct ggggactccg gctggacgac
     4441 ctcaattggg aacggaggaa gtattcgggc ctggcgagct acggctcggc caagctggcc
     4501 cagcttcttt ccatgatcgt gttcgacgag cgcctccggg gcacgggggt gacgatcaac
     4561 acgatgcacc ccggggccgt gaagaccgag accggacagg agaacggacc tgcgtaccgt
     4621 tggttcaagc ggaatttctt cgacaagacc ttgaaatccc cggagctctc cgcggaggca
     4681 ctatactatc taggtgcggc caaggagttg gagggggtca gcgggaagtt cttcaacctg
     4741 accaccgagg aggaaccggc tccgcccgcc cgggaccggg aagccgcgta cgagttgtgg
     4801 cgggaaaccc tcaagatgac aggtcttgag gatgcggaat ccgtacccgg gcacgatccg
     4861 aaataaggaa gctcgcggcc gctcctttta gggccgctcg tactccagcc gtcccccgat
     4921 caccgtcgtc ttcacgaggt tcatgccgct gtggtaggcc aggaaggccc gggatggggc
     4981 gtccaggatg actaggtccc cccgcttgcc cggttcgatg gagccgacct cgccggccag
     5041 gcccagggcg gcggccccgt tcaaagtcag ggcgcagaga gcttcgtcca cggacaggcg
     5101 catgtacagg accgccaggg cgaagatgag ggggatggac tggctgtagc aggacccggg
     5161 attcaggtcg ctggccaggg ccacggcgca gcctgagtcg atcatgcccc gggcgtccgc
     5221 gtagggctcc cggaggacga aggccgtcag gggcaggcag gtcgccaccg tggaggaccg
     5281 ggccagggcc tcgtagtccg agggcacgca cttcaggagg tggtccgcgc tggcggctcc
     5341 cagctcggcc gccagggccg caccgccgat cctctcgatc tcgtccgcgt ggaccttgag
     5401 cccgaagccc gcggccttgg cggcctcgag gtagcgccgg gaatccgcca gctcgaagac
     5461 ccccttctcg cagaagacgt cgcagaaccg ggcgagcccc cgggccttga ccgcgggcag
     5521 catgtcccgg atcacgtagt ccatgtactc cgaggtgcgg cccgcgtact ccggcggggt
     5581 cgagtggggt cccaggaagg tggggacgat ccggacgggc tgttcccggc ccaggatgtc
     5641 catggcttcc agctggcgca gctccgtggc caggtccatg ccgtagccgc tcttgccctc
     5701 cacggtggtg acccccaggg agaccatggt cccgaggcgc ctccggccga tcgcgacgag
     5761 ctcgtccagg cttgccgccc gagtggcgtc catggacctc cggatgcccc cgccccggcg
     5821 gtggatctcc atgtagggca ggcccccggc ccgccagagg aactcctcgg agcggtcccc
     5881 ggcgaagacg aagtgggtgt gggaatccac gaagccgggg accacggtcc ccccgcccgc
     5941 ggacaggacc ggtacatcct cgggaatcgc gggggcttcc gaggcgggac ccgcccaggc
     6001 gatccggccg tcccggatca ggaccgccgc gtcgggcacc gaccggacct cggccatctc
     6061 cgggccccgt tgggccttgc cgcccgtcgg cgtcgcaagt tcgcggatgt cccgaacgaa
     6121 gagatcggcg gtcgacacgg cgggcctcac ttcctgggta cgggcttgcg gcccgtgaac
     6181 agggggtccg cccccagggt cccggaaaga ccctccacga aggcggggtc caggaccacc
     6241 cgaagttcct tctcggcggc cttgaggatc ttgtccagcc gcggggcgaa ggcggggtcc
     6301 gtgaaccgct ggtcggcctt ggccttgcgg atctcgacga tggaccgttc caggaaggtc
     6361 acctgcttgt cccggtagcg gtccaggcgc tccttgtacc agtccgaggc gaggacgtag
     6421 tcccggtcga agagccttcg cagctccgga tcgtcgatgg tcttgccgtt ccaggtgccc
     6481 tcggccatga cgtgcaggat ggccgccagg ggcgggatgg ccgcctccac gctcccgtcc
     6541 gccaggtaat cccgggccac cttggcctgg gcctccgcga tgttctccac cccgtccgcg
     6601 aattccgcca ggtcctggag ctcgggcttg aggaagtcct cggggaagac cgaactgggg
     6661 gtgtcgaaga tgcggcccag gaaggtggtg gcgaagaggg aggtgatccg gtagcccagg
     6721 cggcttgcgg ggatccgccg gccttggtag tcgaagtcct cgatcttctc caggtagccg
     6781 ttggcgatga ggaaggccgg gtccttctcc tcggggtcca tccgggacca gagctcgggc
     6841 accaggaggg acacgtcgtg gttcaccttg tacttcccgc cgatgtgtcc cgcggcgctg
     6901 gagaagcccc cgtatccggt caagatgtac gagaggaggg ccgcgttcag gtccgtggtc
     6961 ggaaccaggg cgttgaaggg acccttggtc agggcgccct cgctgccggc cccggtggtg
     7021 gacgggctct tcccggtcag ggagcagacg aagtccatga agagctccgg gagctcctgg
     7081 tagtggatgg ggccgtagac cgccaggggg cggatctttc cggccgggtc cgcgggattg
     7141 ttcctgcggc ccggtagggc ggcgcccacg ggatggagta cgggcttgtc ctcgggaata
     7201 cggtagaaga ggcggtgccc caggtccgca aggtagcgct tgcagggctc gatgtggttg
     7261 gggtccagct ggaggtagcg ggggttcttg gagggcttgc cgtccaccag gcgggggcgg
     7321 tcgctggcca cgaaccagtc gaagccgtcg tccttctccg cgtcctcgat gaagcgccgc
     7381 atgggctccg tgtacgcgga gaactcgatg gtcttctcga tcatggcgtg ggccttggac
     7441 cgggagaggg gttcgaagtt ggagatgaag ttgtccggcc gggccatgtc cttttcggcc
     7501 tgcttgtcgt agccccggat gaccgcgtcg tcggggcgct ggaagaaccg cgcctccgcg
     7561 ttccggcaga acttgaccga tatccccggc gatgccttct cgagctccgg cagggaggcc
     7621 ttgggcagga cgatgctggc ggtgatgtcg tcctcccact ggaccttctc cgcgggcagg
     7681 tagtcctggc ggagcttgaa gatcctccgg cggccgtcct gggagtaccc cacccggagg
     7741 taggagccca ggaccgggcg tccctcgaac ttgaggatgt tccccgcggt cccgttcacc
     7801 gtgtccaccg tgaagtgctt ggcccagtcc tccccccagt cgggcacgta gaagcgcttg
     7861 atcaggaaga ccagggcctt gatccgctcg gggatggtct tgagccagtc gttgtactcg
     7921 tccgtgtaca agggcgaggg ggacaggagc ttgatcacgc tgcccaggga acgttggctg
     7981 gagagcaccc tgcgctcgtc gggccggtgc ttctccttgg tggcgcggaa acggccggag
     8041 tagtcccgct ccaggatgtc ccggacctgg gccatgtccg cctcgtagtc cccgatgatg
     8101 aaggggaaat actcgatggc gtcccagatg ctcttggaaa tctcagactt gccgcccccg
     8161 gagaccgtgc agggcttgtg gcacaagagg cccgtggcca gggtgcccac gagccgccag
     8221 gccccggagc ccgcgtggcg gttcatgccg atcttgtagc ccgtggggtg gatgtaggtc
     8281 tcgttcggca ggatccgcag ggagtactcc ttgccgtccc gggtccaccg ggcctgctgg
     8341 tcctccaggg agatctgggc gttttccgga aggtagacga tgttcgggaa ggtacggtcc
     8401 cgggcgaagc cgtcgggatg ttcctccagg tggtccttga ggaggctcac cacctcgtcg
     8461 aagttatggc cccgggcctt gaggttggcg tccggaacga agcgggtgcc caggttgtag
     8521 gtcgggaaga ccagggctcc cccggcgtgc tcctcctcgg tcatgcccag gaggttggag
     8581 gagaaggaga tctgggtctt cacctccttc ttggagtacc cgaagtagtt gtccgcgatg
     8641 atggtgaaga tcaccccccg ctcattccgg gcgcagagct tgaagggctt gccctcgttg
     8701 tagagctcct cgggatcctt ccaggccatc ccgtcccgcc tctggcgctc ggaggcgtcg
     8761 tcccagcggg gaagccccag gtcccgcttc ttgaggccca cgaggtgggt ggccaggagg
     8821 atgcagcccg tgtggccggt ccagtgcagg gggtccaggg cggagtcgtt ctcggcgatg
     8881 taggggtcgc cggagttccc gaagatgctc tccacgaagt ccaggttggc cgcgagggag
     8941 cccggcgcga agaagcggat ctccatggag cgctccggac agatccccgg cacctcgggc
     9001 tggaccacgg ggcggagcag gagggagagg aaggtccggc cgggcttggg ctcctgggcc
     9061 gtgaagggga gggttagcat ctcctgcggg ggggtgacgg cggcggccag gatgcggccg
     9121 aagaccgcct tgggcacggc tttcttgtcc gggggcaccg gaagcccccc gtcggccacg
     9181 tggaagaccc cctccgtggt ccgccggtcg ttggcggggt tgtggaggat cccgttccgg
     9241 atgcggtagg aggtcagtgt gggggaggag tacgtggagg cttcgggggg aagggacagc
     9301 tcccgggcca tgccgtagcg gtccagggtc agggtgttgg aggggagacg ggggatctcc
     9361 ggcagccccg tgtcggccag gtagtcggac aggaaggcct ggatgcgctg gtccgccggg
     9421 ggcaggtagc cggtgaggag gcggtccttc tcccggtagt tctcgatcag ctcccgggcc
     9481 agctccacaa aggccgtgcc ttcccgggaa ta
//
LOCUS       DAOTEI010000137         9469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5133_length_9469_cov_5.615466, whole genome shotgun sequence.
ACCESSION   DAOTEI010000137 DAOTEI010000000
VERSION     DAOTEI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5133_length_9469_cov_5.615466"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="P5313_12350"
     CDS             <1..352
                     /locus_tag="P5313_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5616 domain-containing protein"
                     /protein_id="HRY81202.1"
                     /translation="AIALIGRILGLYESARATWYLDAPVSNSGRLRAILYEEAEKGGW
                     DWRVEVTNGVDRVVAESDAVVLVVSEETGALSLAYDAKLHYNLTASLVQRRLAALLDF
                     SETSAAEEEGPDEP"
     gene            342..1298
                     /locus_tag="P5313_12355"
     CDS             342..1298
                     /locus_tag="P5313_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRY81203.1"
                     /translation="MSRKPILERILENWPAKILSFMAAVLLFSFNQLNRLEERPLAVP
                     LRLVGSEDLAPSSAYPRTVRIVLKGEANSIFAVQEGDLEAVLDLSEFKAEGVYRVPVV
                     VEKRGSAVGIDPLEIRVDPSEVAIALEQRITRSVPVVPSFRGFPDPGFELASSELVPP
                     TAEIEGPASLVRKATDVTTEFIELSGRSEDFSAVLRLQQRDPLLGFTGPGTVEFRAKI
                     QRALAYRSFEGLPIRLEGLAPGLAVAGELPSGTLRVAGARNDLSFLTPAPDALYADLS
                     LIDEPGVYTVPVGIRVPPGVSPEAWTPGAVTLAVVRSSEVSR"
     gene            1295..1672
                     /locus_tag="P5313_12360"
     CDS             1295..1672
                     /locus_tag="P5313_12360"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRY81204.1"
                     /translation="MILGIGVDVVHVRRIEHWLTVPGLPERFFHPEELSAARSRGDTL
                     SLSLAARFAAKEAFGKALGTGLAGIKLRDIQVENTPYGRPDIVLAGTALEKFQAAGGG
                     RILLSLTHERDNAIAMVVIEDGR"
     gene            1669..2553
                     /locus_tag="P5313_12365"
     CDS             1669..2553
                     /locus_tag="P5313_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HRY81205.1"
                     /translation="MKGLLMKGKEDKDKKITFFSKEAVRCPVCGAAFHREELFSGRVN
                     AGDLTDELHRQYIPMRDFGEVQPLVYDVTVCPSCFYAALRSDFSAAPQKALDVLEGET
                     SARIAQVQKVFEGLDFQGSRRILEGAASYYLAVLCYEHFPKDFSPTLKGAMCCLRAAW
                     LCGHLHEKAPNENFDYLALVFYRKARVLYRGAIEREQSGKEPIGGARWMGPDTDKNYG
                     YEGALYLSGVLELKYGNRGNPAYREKILDESKRTIAKMFGLGKRSKAKPGPLLDKARD
                     LYDRIKAELHQDDDSDDE"
     gene            2546..3337
                     /gene="truA"
                     /locus_tag="P5313_12370"
     CDS             2546..3337
                     /gene="truA"
                     /locus_tag="P5313_12370"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRY81206.1"
                     /translation="MSERTVRLDLSYDGTDFSGWQRQKNARSVQEELEKALGALHGHP
                     VEAVGAGRTDAGVHAERQTAHFRSDMPSIPGDRFRLALNRFLPRDVRILESREADPDF
                     HARYDARLRTYRYFIETSRECPPGRARYAWHLRRVPDLRALNAMASVLIGETDFTAFS
                     AARDPSENRYRFVHSASWRFECGQAVFEISANAFLLRMVRSLVGSMVEYQGEGRDPSW
                     MREVLESRDRSRAGSTAPACGLFLWNVEYYDKPTRAGRAGDGFID"
     gene            complement(3517..4362)
                     /locus_tag="P5313_12375"
     CDS             complement(3517..4362)
                     /locus_tag="P5313_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRY81207.1"
                     /translation="MQGSAPVVRALDNLNLRKSLDDLITLEALGTALKLAGILVLGLI
                     VVRVAVAVTRRFSRKTLSPRTSELLVKFVRYTGFFLVAVNLANAAGLDLSALLGAAGI
                     AGIAIGFAAQTSISNLISGIFLLTEKAYAVGDVLDVGGGTVGVVDSIDMLSVKLKTFD
                     NRLVRIPNETLVKSNITNVTRYPIRRLNINITITYGDDIEKARRILLETADANPWILR
                     SPNPIFIVEGFGESGINLFFGVWFTKDEFVDAKNSMYIEIKRRFDEEKVEFAFRTVTV
                     YQKKG"
     gene            complement(4381..5151)
                     /locus_tag="P5313_12380"
     CDS             complement(4381..5151)
                     /locus_tag="P5313_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81208.1"
                     /translation="MWSTFALSPGTTYAIRFPGVNLYFRAEGVLWGVASEFVAKPQRP
                     APAAVVRNPPKTLEWKYSALLGAAELTLRPTLSPRAYEVQLTQAVSILPGVELDGYVF
                     LPLRAEVLAAEGTILASLPLQRRKNSWFGSPQEGVLCEAVVSAFSAAEELGRFLADPS
                     PALAVCPISVRNDMAEGMDLVRLCIPTDILSVYGGEGIYATDRVLCSFTSDGLQVRPK
                     RGMDPLLGRMEVRHAARKGPEERFFSRGMDILRYITGI"
     gene            5309..6268
                     /locus_tag="P5313_12385"
     CDS             5309..6268
                     /locus_tag="P5313_12385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY81209.1"
                     /translation="MASFEGIVEGPGNARLDVYVSETLRLLSRSQLKARLRSAAVNGR
                     PAKPSRVLREGDRLSLTWEEEIPAGLEPEDIPLSVLYEDERVIVVDKAQGMVTHPAHG
                     NWSGTLANALAGRRPGGGAEAGDRGSAPGAAPDRAGIVHRLDKDTSGLLIAAKDAEAQ
                     EFLSAQFRDRRVLKEYLALCSRPLPAPSGRVEGRMGRDPRDRKRFAPVETGGKLAVTD
                     WKVLESLGPYSLVALRPRTGRTHQLRVHMKALGCPILGDPLYGRRDLRFPEATLMLHA
                     RRLRILLPGRSEPSTFTAEVPERFRRVLAALRAEFGESNTVSG"
     gene            complement(6484..6774)
                     /locus_tag="P5313_12390"
     CDS             complement(6484..6774)
                     /locus_tag="P5313_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY81210.1"
                     /translation="MAKKIYVGNVSFSTTESSLKNLFSQYGEVLSANVVMDRVTGRSK
                     GFAFVEMADDAAAQSAIQALDGKEFEGRQVRVNEAMDKPRSSGGYGDRSNRY"
     gene            6974..8503
                     /gene="rny"
                     /locus_tag="P5313_12395"
     CDS             6974..8503
                     /gene="rny"
                     /locus_tag="P5313_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRY81211.1"
                     /translation="MKAIVYLALPLTGLILGWTIRWLYARFQLSSAEQSAERIKQEAV
                     KQAEAEKREILVEAKDQLIRERNQQEREVRERRAELQRFERRILQKEESLDKKVQSLD
                     KQEQVLQTREKEAQDKEEFLAGQEERYRKELERISGLSADDAKKIIIQGLENEAKRDA
                     QVIINKIEQEAQVASDRKARDILVTTIQRLATEVTTEVTVTSVSLPSDEMKGRIIGRE
                     GRNIRTLETLTGVDVIIDDTPEAVVISCFDPVRREIARVSLERLISDGRIHPARIEEV
                     VQKVTREISQKIFEEGEKVVFDLGLHNLNPELIKALGRLHYRTSYGQNVLAHSKEVAV
                     IAGMLAAEIGLNREIAKRGALLHDIGKGIETDSDQNHAEVGMELARKLGEDPRVINAI
                     GSHHADVEPSCPESVIVQMADAISAARPGARRETLDNYIKRLENLENIAESFSGVDKA
                     YAIQAGRELRILVNNEQVSDDDAKDLCKSIAKKIETDLRYPGRIKVTIIRETRIVEYA
                     R"
     gene            8678..9454
                     /locus_tag="P5313_12400"
     CDS             8678..9454
                     /locus_tag="P5313_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRY81212.1"
                     /translation="MQDTVRVLMLGDVIGDPGLRALFAGLPGLIKKTRADLVVANGEN
                     AAGGFGITREAAERMFSLGIDVITTGNHVWEKKDIDKLLDSEPRILRPANYPAGAPGS
                     GFRAFEKDGVPWLVLSLQGRERLYPIDCPFRTAKEILKRHKSHLAVIDFHAEATEEKE
                     ALGFHLDGEASVVAGTHTHVQTADERILPGGTGYITDLGMSGPADSVIGVKTEICLRR
                     SLTQMPLKMESAEGEAGIQGALFEVDVRSRRTLSILRIRS"
BASE COUNT         1671 a         3076 c         3207 g         1515 t
ORIGIN      
        1 ggcgatcgcg ctcatcggcc ggattctcgg tctgtacgaa tccgctcggg cgacgtggta
       61 cttggacgcg cccgtatcca acagtggacg gctgcgggcc attttgtatg aggaggccga
      121 gaaaggcggg tgggactggc gggtcgaagt gacgaacggt gtggaccgcg tcgtggcgga
      181 atccgacgcc gtggtcctgg tggtctcgga ggagaccggg gccctctccc tggcctacga
      241 cgccaagctc cactacaacc tgacggcgtc cctggtccag cgccgcctgg cggccctcct
      301 ggacttctcc gagacctccg ccgcggagga ggaaggtccc gatgagccgt aagccgatcc
      361 tggagcggat cctggagaac tggcccgcca agatcctgtc cttcatggcg gcggtgctcc
      421 tgttctcctt caaccagctg aaccgtctgg aggagcgccc ccttgccgtg cccctccggc
      481 tggtggggag cgaggacctg gccccctcct ccgcctaccc ccggaccgtg cggatcgtgc
      541 tcaagggaga ggccaactcc atcttcgccg tccaggaggg tgacctggag gcggtcctgg
      601 acctctcgga gttcaaggcc gagggggtct accgggtgcc cgtggtggtg gagaagcgcg
      661 gcagcgccgt cgggatagat cccctggaga tccgggtgga tccctccgag gtggccatag
      721 ccctggaaca gaggatcacc cgctccgtgc ccgtggtccc gagcttccgg ggcttcccgg
      781 accccggctt cgagctggcc tcgtccgagc tcgtgccccc gaccgcggag atcgagggtc
      841 ccgccagcct cgtgcggaag gccacggacg taaccaccga gttcatcgag ctgtccggac
      901 ggagcgagga cttcagcgcc gtcctccgcc tccagcagcg ggatcccctc ctgggcttca
      961 cggggccggg gacggtggag ttccgggcca agatccagag ggccctggcc taccggagtt
     1021 tcgaagggct tcccatccgg ctggaaggcc tggcgccggg gctggccgtg gctggggaac
     1081 ttccctccgg caccctgcgg gtcgccgggg cccggaacga cctctccttc ctcacgcccg
     1141 ccccggacgc cctgtacgcg gacctgtccc tgatcgacga gccgggagtg tataccgtgc
     1201 ccgtggggat ccgggtgccc ccgggggtga gcccggaggc ctggaccccc ggcgccgtca
     1261 ccctggccgt agtccgaagc tcggaggtgt cccgatgatc ctgggtatcg gcgtggacgt
     1321 cgtgcacgtc cgccggatcg agcactggct gaccgtgccg ggcctcccgg aacggttctt
     1381 ccatcccgag gagctctccg cggcccgcag ccggggagat accctctccc tatccctggc
     1441 ggcgcggttc gcggccaagg aggccttcgg caaggccctt ggcacgggcc tggcggggat
     1501 caagctccgg gacatccagg tggagaacac cccctacggg cggccggaca tcgtcctggc
     1561 cggtacggcg ctggagaaat tccaggccgc cggaggcggg aggatcctcc tgtccctgac
     1621 ccacgaacgg gacaacgcca tcgcgatggt ggtgatcgag gacggccgat gaagggactc
     1681 ttgatgaagg ggaaggaaga caaggacaag aagatcacgt tcttctccaa ggaggccgtg
     1741 cgctgtccgg tctgcggggc ggctttccac cgggaggagc ttttttccgg gagggtgaac
     1801 gcgggggacc tcacggacga gctccaccgg cagtacatac ccatgcggga cttcggggag
     1861 gtccagcccc tggtctacga cgtgaccgtc tgcccctcct gcttctacgc cgccctcagg
     1921 tccgattttt ccgcggcccc ccagaaggcc ctggacgtcc tggaggggga gacctccgcc
     1981 cggatagccc aggtccagaa ggtgttcgag ggcctggact tccagggttc ccggcggatc
     2041 ctggagggag cggccagcta ctacctcgcg gtgctctgct acgagcactt ccccaaggat
     2101 ttttcgccca ccctgaaggg ggcgatgtgc tgcctccggg cggcctggct ctgcggccac
     2161 ctccacgaga aggccccgaa cgagaacttc gactacctgg ccctggtctt ctaccgcaag
     2221 gcccgggtcc tctaccgggg ggcgatcgaa cgggagcagt cgggcaagga acccatcgga
     2281 ggggcccggt ggatggggcc ggacacggac aagaactacg gctacgaggg ggccctgtac
     2341 ctctccgggg tcctggagct caagtacgga aaccggggga acccggccta ccgggagaag
     2401 atcctggacg agtccaagcg caccatcgcc aagatgttcg gcctgggcaa gcgatccaag
     2461 gccaagccgg ggcccctcct ggacaaggcc cgggacctct acgatcggat caaggcggag
     2521 ctccaccagg acgacgattc cgacgatgag tgagcggacc gttcgactgg acctgtccta
     2581 cgacgggacg gatttttccg gctggcagag gcagaagaac gcccgctccg tgcaggagga
     2641 gctggagaag gccctggggg ccctccacgg ccatcccgtg gaggcggtcg gggcgggacg
     2701 gacggacgcg ggggtccacg cggagcgcca gaccgcgcac ttccggtcgg acatgccctc
     2761 catacccggg gaccgtttcc gcctggccct gaaccgtttc ctgccccggg atgtccgcat
     2821 cctggaatcc cgggaggcgg atccggattt ccacgcccgg tacgacgccc ggctccggac
     2881 ctaccgatac ttcatcgaga catcccggga gtgccctccc gggcgcgccc ggtacgcctg
     2941 gcacctccgg cgggtcccgg acctgcgggc cctgaacgcc atggcctccg tcctgatcgg
     3001 ggaaaccgac ttcaccgcct tctcggccgc gagggatccc tcggaaaacc ggtaccgctt
     3061 cgtgcactcc gcctcctgga ggttcgaatg cgggcaggcg gtcttcgaga tctccgcgaa
     3121 cgccttcctc ctgcgcatgg tccggtccct ggtgggatcc atggtggagt accagggcga
     3181 ggggagggat ccttcctgga tgcgggaggt cctggagtcc cgggaccgga gccgggccgg
     3241 gtccacggcc cccgcctgcg gccttttcct ctggaacgtg gaatattacg acaagcccac
     3301 ccgcgcgggc cgcgccgggg atggttttat tgattaaacc acggagggcg ctaggctgac
     3361 tatatgtcag cctagcggca acgaagacct gtggtcttcg tcgaggttgt cggagtcggg
     3421 atcgccgaag ctcgcttcgg cgccgaccgg ccggcagcgc cggccggtcg ccctccgtga
     3481 gctcgtgtcc tccgtggtga gtcctgcaaa ggcggcctat ccctttttct ggtacaccgt
     3541 cacagtccgg aaggcgaact ccactttctc ctcgtcgaag cgcctcttga tctcgatgta
     3601 catcgagttc ttggcgtcca cgaactcgtc cttggtgaac cataccccga agaagaggtt
     3661 gatcccgctc tccccgaagc cctccacgat gaagatgggg ttgggggaac ggaggatcca
     3721 gggattggcg tccgcggttt ccagtaggat ccggcgggcc ttctcgatgt cgtccccgta
     3781 ggtgatggtg atgttgatgt tgaggcggcg gatggggtag cgggtgacgt tggtgatgtt
     3841 cgacttgacc agggtctcgt tggggatccg gaccaggcgg ttgtcgaagg tcttgagctt
     3901 gaccgagagc atatcgatgg aatccacgac ccctacggtg ccgccgccca cgtccaggac
     3961 gtcccccacg gcgtaggcct tctcggtcag gaggaagatc ccgctgatca ggttggagat
     4021 ggaggtctgg gccgcgaagc cgatggcgat ccccgcgatc ccggcggccc ccaggagggc
     4081 cgagaggtcc aggcccgccg cgttggccag gttcacggcc acaaggaaga agccggtata
     4141 ccgcacgaac ttgaccagga gctccgaggt cctgggggaa agggttttcc ggctgaacct
     4201 ccgggtcacc gcgacggcca cccgtacgac gatcaggccc aggaccagga ttcccgcgag
     4261 cttgagggcc gtgcccaggg cttccagggt gatcaggtcg tccagggatt ttctcagatt
     4321 caggttgtcc aaggcccgga ccacgggggc agatccctgc atggcggctc ctcctggggg
     4381 ttaaattccc gtgatgtacc ggaggatgtc catcccccgg gaaaagaacc gttcctcggg
     4441 gcccttccgg gccgcgtggc ggacctccat ccgtcccagc agggggtcca tgccgcgctt
     4501 cggccggacc tgcaagccgt cggacgtgaa ggagcacagg acccgatccg tcgcgtagat
     4561 cccctccccg ccgtagacgg acaggatgtc cgtgggaatg cagagccgga ccaggtccat
     4621 gccctcggcc atgtcgttcc gcacggagat gggacagacc gcgagggccg gggaaggatc
     4681 cgccaggaat cttcccagtt cctccgcggc gctgaacgcg gataccacgg cctcgcagag
     4741 gacgccctcc tggggggacc cgaaccagga gttcttccgg cgctgtaggg gaagggaggc
     4801 caggatggtc ccctccgcgg ccaggacctc cgcccgcagg ggcaggaaca cgtacccgtc
     4861 cagttccacc cccgggagga tggataccgc ctgggtcagc tggacctcgt aggcccgggg
     4921 ggagagggta gggcggaggg tgagctccgc ggcccccagc agggcggagt acttccactc
     4981 cagggttttc ggggggttcc gaaccaccgc ggccggggcg gggcgctggg gcttggcgac
     5041 gaactccgag gcgacgcccc agaggacccc ctcggcgcgg aaatagaggt tgacccccgg
     5101 gaaccggatc gcgtaggtcg ttccgggtga gagggcgaag gtggaccaca tggcttcctc
     5161 cggcgtaccc caaagtcggg ctcggaccgc gccattgccc ggaaccaccc ggggatcgta
     5221 tactcggacg cgcgcggccg aaccattacg atacaggata acgcgggccg gctcaactgc
     5281 ccgatccgaa atccgcgcgg gaagggatat ggcatccttc gaaggtatcg tcgagggacc
     5341 ggggaacgcc cggctcgacg tgtacgtgtc cgagacgctg agactcctga gccggtccca
     5401 gctgaaagcc cgcctccgct cggccgcggt gaacgggaga cccgccaagc cctcccgggt
     5461 gctccgggag ggggaccgac tttcacttac atgggaagag gagatccccg cgggcctgga
     5521 gcccgaggac atcccccttt cggtcctcta cgaggacgag cgggtgatcg tggtggacaa
     5581 ggcccagggc atggtgaccc atcccgccca cggcaactgg agcgggaccc tggccaacgc
     5641 cctggccggc cggcggcccg gcggcggcgc ggaggcgggc gaccggggat ccgccccggg
     5701 ggcggcgccg gaccgggccg ggatcgtcca ccgcctggac aaggacacct cggggctcct
     5761 catcgcggcc aaggacgcgg aggcccagga gttcctttcc gcccagttcc gggaccgccg
     5821 ggtcctgaaa gagtatctcg ccctctgctc ccgccccctt cccgccccgt cgggcagggt
     5881 ggagggccgg atgggccggg atccccggga ccggaagcgc ttcgcccccg tggaaaccgg
     5941 aggcaagctc gccgtcacag actggaaggt gctggaaagc ctcggaccct atagtctcgt
     6001 cgccctgcga ccccggaccg gtcggaccca ccagctccgc gtgcatatga aagccctggg
     6061 ctgcccgatc ctgggggacc ctctctacgg gcgccgggat ctgcggttcc cggaggccac
     6121 cctcatgctc cacgcccggc ggctccgcat cctcctgccc gggcgctccg agccctccac
     6181 gttcacggcg gaggtcccgg agcgcttccg ccgggtcctg gcggccctcc gcgcggaatt
     6241 cggggaatcg aatacggttt ccggataatt ccgacaggat ggacaggatt ggatccagga
     6301 tgaacaggat gggaaggaga acaggaggaa tcgcgttttt ttcacttctt tctttgcttc
     6361 agtatcctgt tcatccgttc agaatcttgt acatcctgtc tacattttta taacaaagcg
     6421 ccccgccggg gggcggggcg gaagtgcggt actcggaagc cgcgcgggtc gcggcggacg
     6481 ggttcagtac cggttggagc ggtctccgta tcccccggag gaccggggct tgtccatggc
     6541 ctcgttcacg cggacttggc gtccctcgaa ctctttgccg tccagggcct ggatggccga
     6601 ctgggccgcc gcgtcgtccg ccatctccac gaaggcgaac cccttggacc ggcccgtgac
     6661 gcggtccatg acgacgttcg cggaaagcac ttccccgtac tgggagaaga gatttttcag
     6721 gctggattcg gtggtggaga agcttacgtt ccccacgtag atctttttag ccatgccggg
     6781 aatcctcggg gtggtggccg atgaacggcc cgcgattcga cccgcccagg gcggttcgac
     6841 gtccttcctt gaagagcgga cgcgcttaaa ctatagaccc gcctgcccgg gccgtcaatt
     6901 ccgatccacg ttctttcttg acaaatggga gtcgattcgt ataatcaatt cctaatagta
     6961 agtaggagga gccatgaagg ctatagtgta cttagccctt cctcttactg gtttgatcct
     7021 gggatggacg atccgctggc tgtatgccag atttcaactt tcctcggccg aacagtcggc
     7081 cgagcgaatt aaacaggaag cggtcaagca agccgaggcc gagaagaggg aaatcctcgt
     7141 cgaagccaag gaccaactca ttcgggagag aaaccagcaa gagcgcgagg ttcgggaacg
     7201 cagggccgaa ctccagagat tcgagcgacg gatacttcag aaggaagaga gtctcgacaa
     7261 gaaggtccaa tccctggaca agcaggagca ggtcctgcag acccgggaga aggaagccca
     7321 ggacaaagag gaattcctgg cgggccagga agagcggtac cgcaaggagc tcgagcgcat
     7381 ctccggcctt tccgccgacg acgccaagaa gatcatcatc cagggcctgg agaacgaggc
     7441 caagcgggac gcccaggtca tcatcaacaa gatcgagcag gaagcccagg tcgcctcgga
     7501 ccggaaggcc cgggacatcc tggtcaccac catccagcgg ctggccacgg aggtgaccac
     7561 cgaggtcacg gtgaccagcg tcagcctgcc ctcggacgag atgaagggcc ggatcatcgg
     7621 ccgggagggc cggaacatcc ggaccctgga gaccctgacc ggggtggacg tcatcatcga
     7681 cgataccccc gaggcggtcg tcatctcctg cttcgacccg gtccgccggg agatcgcgcg
     7741 ggtgtccctg gaacggctca tctccgacgg gcgtatccat cccgcccgga tcgaggaggt
     7801 cgtccagaag gtcacccggg agatcagcca gaagatcttc gaggaggggg agaaggtcgt
     7861 cttcgacctg ggcctccaca acttgaatcc cgagctgatc aaggccctgg gccgcctgca
     7921 ctaccggacc agctacggcc agaacgtcct ggcccactcc aaggaggtgg ccgtcatcgc
     7981 gggcatgctg gccgcggaga tcggcctcaa ccgggagatc gccaagcggg gagccctgct
     8041 ccacgacatc ggcaagggca tcgagacgga ctcggaccag aaccacgcgg aggtcggcat
     8101 ggaactggcc cgaaagctgg gagaggatcc ccgggtcatc aacgccatcg gatcccacca
     8161 cgcggacgtg gagccctcct gcccggagag cgtcatcgtc cagatggcgg acgccatcag
     8221 cgcggcccgg ccgggagccc gccgggagac cctggacaac tatatcaagc gcctggaaaa
     8281 cctggagaac atcgcggaga gcttctccgg ggtggacaag gcctacgcca tccaggccgg
     8341 ccgggagctc cggatcctgg tgaacaacga gcaggtctcc gacgacgacg ccaaggacct
     8401 gtgcaagtcc atcgccaaga agatcgagac ggacctgcgg taccccggac ggatcaaggt
     8461 cacgatcatc cgtgagactc gcatcgtcga atacgcgcga tgaaaaagcg ctaggcgctt
     8521 tttcatcgcg gcaacgaagc ccttcgggct tcgtcgaggt ttgcggatcg acaagcgagg
     8581 gagcggcgcg gggcgccgct ccctcttttt cgggttgtag aagcgctcca aggcttcaac
     8641 gcccgaatgg gcccgagtct gggccgggga gaacggcgtg caggataccg tccgggtgct
     8701 gatgctggga gacgtgatcg gggacccggg cctccgggcc ctcttcgcgg ggctcccggg
     8761 gctgatcaag aagacccggg cggacctggt ggtcgccaac ggggagaacg cggccggagg
     8821 cttcgggatc acccgggagg ccgcggaacg gatgttttcc ctgggaatag acgtgatcac
     8881 cacgggcaac cacgtctggg aaaagaagga catcgacaag cttctggatt cggagccccg
     8941 gatcctaagg cccgccaact accccgccgg ggccccggga tccgggttcc gggccttcga
     9001 gaaggacggc gtgccctggc tcgtcctgtc cctccagggg cgggagcgac tgtatcccat
     9061 cgactgcccc ttccgtacgg ccaaggagat cctgaaacgg cacaagagtc acctggccgt
     9121 catcgatttc cacgcggagg ccacggaaga gaaggaggcc ctgggcttcc acctggacgg
     9181 cgaggcctcg gtcgtggcgg ggacccacac ccatgtccag accgcggacg agcggatcct
     9241 ccccgggggg accggctaca tcaccgacct gggcatgtcg ggtccggccg actcggtcat
     9301 cggggtcaag acggagatct gcctccgccg gagcctcacc cagatgcccc tcaagatgga
     9361 atccgccgag ggcgaggccg ggatccaggg ggccctcttc gaggtggacg tccggtcccg
     9421 caggaccctg tcgattctcc ggatccgctc ctgatgcgga tgtaccgta
//
LOCUS       DAOTEI010000138         9463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5142_length_9463_cov_5.365434, whole genome shotgun sequence.
ACCESSION   DAOTEI010000138 DAOTEI010000000
VERSION     DAOTEI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5142_length_9463_cov_5.365434"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..2058
                     /gene="glmS"
                     /locus_tag="P5313_12405"
     CDS             235..2058
                     /gene="glmS"
                     /locus_tag="P5313_12405"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRY81213.1"
                     /translation="MCGIVGYTGPRRAAPVLVEGLKRLEYRGYDSAGIAVQSGSDLKV
                     IKTVGKIARLKAIVPADLPGTCGIGHTRWATHGGVTDANAHPHRDPTGKIVLVHNGIV
                     ENYKGIKEKLQAKGAVFLSETDTEVIAHLIARYYEGDLEKAVKAAVQRLEGTYGIAVM
                     HADEPGRIVGARKGSPLVVGVGEGEMLLASDVTAMVAHTKRVVYMNDGEVADIRPDGF
                     TITDLDDRPVDKRIDSVDWDLGEIEKEGFSCYMEKEIFEQPESIGRALSGRLDAEFAT
                     GKLGGLNLTPQQLHSMQRIKIIAAGTSWHAGLVGAYLLESVARIPAAAELASEIRYRN
                     PVVEPNTLFLAVSQSGETADTLYAMREVRRKGGTVLGICNVVGSTIARESDGGVYVHS
                     GPEIAVASTKAFTSQLAAFYTLTLLLARMHDMSPTEGRRFMEALRSVPDKVARTLTLR
                     DQIQAIAKKYAKARDFLFLGRNLMYPLALEGALKLKEISYIHAEGYGAGEIKHGPIAL
                     VSPETPSVFLVPDDFLREKTLSNMKEIKARGGPVIAFGVEGDEDVRELADDFIGVPKI
                     DERFYPFLMSAPLQLFAYFCALELNRDVDQPRNLAKSVTVE"
     gene            2051..2551
                     /locus_tag="P5313_12410"
     CDS             2051..2551
                     /locus_tag="P5313_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81214.1"
                     /translation="MNNVRWAVWVLAAAVCAAGLSPPASAQTALARAILVRGTLQVRG
                     VPHLPANAVPALYGEYRLDAAPVRVWVVREELFLTPEWEEIPFRSPPGTRIRAYSRTV
                     GTREAPDTAFALRDSAYWILAEVPAALSNPAAFLEALAARLAYFAAQAKSPGDLSLPA
                     VLEYRP"
     gene            2727..3884
                     /locus_tag="P5313_12415"
     CDS             2727..3884
                     /locus_tag="P5313_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY81215.1"
                     /translation="MNFRKAAFVVLAIALVLAGCGGKQKADTIKVGWFGALTGDQAVW
                     GENEFNTVKMLFEEYNAKGGIEVGGKKYMLEAIGYDNKGDAQEAVNVTNRLVSQDKVV
                     AILGPNASGNAIPIASTLEQAKVPDIATVATNPKVTVQDGKVKPYNFRVCFIDPYQGA
                     VAAGYAFDKLGAKKAAVLYDVSDDYSQGLTEFFVKSFEGKGGKIVAKEGFTAGDKDFR
                     AQLSKIKAAAPDVIFMPYFFTEVALSATQARELGITATFMGGDGWPSPDLFKLGGKAV
                     EGGIFVNHLDYDQPTVQDYKARYKAKYGKETELNGYLVHDAVLMLVDGLQRAGKVDGE
                     ALAKAFESTDIQGITGRIKISPTTHNPEGKDAAIIKIVDGQYKFLENYAAQ"
     gene            3963..4841
                     /locus_tag="P5313_12420"
     CDS             3963..4841
                     /locus_tag="P5313_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY81216.1"
                     /translation="MTQFLQQLTNGLSIGSVYALMAVGYSLVYSIMNFSNFAHGGVIM
                     LGAYFGFFGLTTFKLPFAAAFALATAGTALVAIVVERVAYKPLRDRKAPFLYFIISAM
                     GASMFLENFVIATIGPNPKPYPPIIPREPIALGDLALGRLDLIMFIVSAACLTLLVLF
                     IDRTRLGKAIQASAYNMKASALMGINTDSVVILVFALGGFLAGVAGVLYGMKYMVYPT
                     IGAITLKSFIAAVFGGLGSLPGAVLGSVLLGVIETLVAGYLSSQYRDLIAFVILIGVL
                     IVRPTGIMGKAVEDKA"
     gene            4850..5782
                     /locus_tag="P5313_12425"
     CDS             4850..5782
                     /locus_tag="P5313_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY81217.1"
                     /translation="MDSYILGILMLAGINLVAVLGLSLLTGFTGLFSFGHAGFMAIGA
                     YVGAFLTVSPARDVPGLGLPLVVGILGGGAAAGIVSWLVGRITLNLKGDYFCIATLGF
                     GEAIRLVFNNVQRFGGSRGWPLGATRNPQDIWVILLVDVVAILLVANLVRSRHGRNMI
                     AIREEELAAQVTGINVFRYKMVSLVTSAVLAGVAGVLLGDYLTFINPKIFNLTKSTEL
                     IIIVIFGGLGSISGSVLGALLLTALPEVLRFFEIWRLVFYGAAVILIMISRPQGLLGG
                     MELTPAGIRKILAERRARQALRAEAAREAEGGSR"
     gene            5779..6549
                     /locus_tag="P5313_12430"
     CDS             5779..6549
                     /locus_tag="P5313_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81218.1"
                     /translation="MSGILEVKGLTKRFGGLAAVDNVSFSVREGGISGLIGPNGAGKT
                     TIFNLITGVYKVTEGSVSFSGSEITNMEPFRIADRGITRTFQNIRLFKNLTVYENVLT
                     ACHMSAKYSIPEALLRLPRYLREEKALRAKAEDLLSIMGLTERRDVQANNLPYGLQRR
                     LEIVRALALDPKLLLLDEPAAGMNPDETEQLMRLISEIRTRFKLTVLVIEHHMDLVMR
                     LCESIVVLNFGQKLAEGTADEIRGDPRVVEAYLGSGGN"
     gene            6551..7270
                     /locus_tag="P5313_12435"
     CDS             6551..7270
                     /locus_tag="P5313_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81219.1"
                     /translation="MLTVRDIHVSYGGIRALKGVSLRVEKGEIVTIIGANGAGKSTLL
                     NAISGFLKLGSGEILYQDKLLPKRPDRVVKAGICHVPEGRLVFANLTVEDNLRMGAYL
                     RRDKGAIAADLDKVYGLFPRLKERQRQIAGTLSGGEQQMLAMGRALMTDGDLVLMDEP
                     SLGLAPLLVQTVFDIILEIRALGKTILLVEQNAYKALDAADRAYVLEQGRIVKKGPAA
                     EVKADPSVRAAYLGTESNTAG"
     gene            7341..7922
                     /locus_tag="P5313_12440"
     CDS             7341..7922
                     /locus_tag="P5313_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRY81220.1"
                     /translation="MTFNWAAFLSYIVLTTYTPGPNNLMSMNNAARFGLRKGFPFNLG
                     IWSAFSLVMILCAVFSAALYAVIPRIQLPMKILGAAYMLYLAWNTLKPAPRGSDREAK
                     GGYWYGFALQFVNPKIFIYGITAMSSFILPNFSAPAQVVAFALLLAFVGFTGNMLWAL
                     FGALFLKLFSRHSGVVRAVMTVLLVYCAVSLFL"
     gene            complement(7944..8963)
                     /locus_tag="P5313_12445"
     CDS             complement(7944..8963)
                     /locus_tag="P5313_12445"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY81221.1"
                     /translation="MNSDIPGRSPRPDGDRPILRILVPVLLAASVLGIAAIGLSGFAR
                     ARDASAMAAVGVPEPLRGLPGYSLEALEGPIKIGIQPGHWRIDELPDELARLRTSTGA
                     AFGSLRELDLNLAVSRILVDRLNAAGYSAELVPAAVPAGYRADLFVSVHADRADRPDR
                     RGWKLAPPWRPSPESRRLAEAMADAFRASGLPEDQGGVTANMRGYFGFSWWRFSNTLS
                     PYTPAVLVEMGFLGNPEDRARMRDNPSLYAGILLEGVRTFLAESDRADLDALVPVLYR
                     GVSAGPRGAAGRASPSLDSAVLERYPPGRYLNPVDSSEGGWFEVFSRPLRRGVWVHES
                     ELDGE"
BASE COUNT         1629 a         3185 c         3041 g         1608 t
ORIGIN      
        1 gtgaatgggt gaatgggtga atgggtgaat gggtgaatgg gtgaatgggt gaatgcaatc
       61 cccccaattc acttattcac caattcactt attcactccc ctgcccgccg tcgggcaaat
      121 cccgcgcctc cgccctgtac aggcccccga tctccgcgta tattgtcaga acgcgggcta
      181 tacgagcctt cagcgtgaac tatacggccg aaaagccgga accatggaga atctatgtgc
      241 ggaatcgtgg gatatacggg gccccggcgg gcggcccccg tcctggtgga ggggctcaag
      301 agactggaat acagggggta tgattcggcg ggcatcgcgg tgcagtcggg gtcggatctc
      361 aaggtgataa agaccgtcgg aaagatcgcc cggctcaagg ccatcgttcc cgcggacctg
      421 ccgggaactt gcggcatcgg gcacacccgc tgggccaccc acggcggcgt caccgacgcc
      481 aacgcccatc cccaccggga tcccaccggc aagatagtcc tggtgcacaa cgggatcgtg
      541 gagaactaca agggaatcaa ggaaaagctc caggccaagg gcgcggtctt cctgagcgag
      601 acggatacgg aggtcatcgc ccacctcatc gcacgatact acgaagggga cctggaaaag
      661 gcggtcaagg ccgcggtcca gcgcctggag ggaacctacg ggatcgccgt gatgcacgcc
      721 gacgagcccg gccgcatcgt gggcgcccgg aagggcagcc ccctcgtggt cggggtgggc
      781 gagggggaga tgctcctggc cagcgacgtc accgcgatgg tcgcccacac caagcgggtg
      841 gtctacatga acgacggtga ggtcgcggac atccgcccgg acggtttcac catcacggac
      901 ctggacgacc ggcccgtgga caagcgcatc gacagcgtgg actgggacct cggggagatc
      961 gagaaggaag ggttctcctg ctacatggag aaggagatct tcgagcagcc cgagtccatc
     1021 gggcgggccc tgtccggacg cttggacgcg gagttcgcga ccgggaagct cggaggcctg
     1081 aacctcaccc cccagcagct tcattcgatg cagcgcatca agatcatcgc cgcgggaacc
     1141 agctggcacg cgggcctggt cggggcctac ctcctggaga gcgtggcccg gataccggcc
     1201 gccgcggagc tggccagcga gatccgctac cgcaatcccg tggtggagcc gaacaccctc
     1261 ttcctggcgg tttcccagtc cggggagacc gcggacaccc tctacgccat gcgggaggtg
     1321 cggcgtaagg gcggcacggt cctgggcatc tgcaacgtgg tgggctccac catagcccgg
     1381 gagagcgacg ggggagtcta cgtccactcg gggcccgaga tcgccgtggc ctccaccaag
     1441 gccttcacca gccagctggc cgccttctat accctgaccc tcctcctggc ccgcatgcac
     1501 gacatgagcc ccaccgaggg ccggcgcttc atggaagccc tcaggtccgt gccggacaag
     1561 gtggcccgga ccctgaccct tcgggaccag atccaggcta ttgccaagaa atacgccaag
     1621 gcccgggact tcctcttcct ggggcgcaac ctgatgtacc ccctggccct ggagggggcc
     1681 ctgaagctca aggagatctc ctatatccac gcggagggct acggagccgg ggagatcaag
     1741 cacggcccca tcgccctggt gagccccgag acccccagcg tcttcctggt gcccgacgac
     1801 ttcctccggg agaagaccct gagcaacatg aaggagatca aggcccgggg aggccccgtc
     1861 atagccttcg gggtcgaggg ggacgaggat gtccgcgagc tggcggacga cttcatcggg
     1921 gtgccgaaga tcgacgaacg gttctatccc ttcctgatgt ccgcccccct ccagctcttc
     1981 gcgtacttct gcgccctgga gctcaaccgg gacgtggacc agcctcggaa cctggccaag
     2041 agcgtgaccg ttgaataacg tccggtgggc ggtttgggtc ctggcggccg ccgtgtgcgc
     2101 cgcggggctc tctccgcccg cctccgccca gaccgccctg gcccgggcga tcctggtccg
     2161 gggcaccctg caggtccgcg gggtgcccca cctgccggcc aacgccgtcc cggccctgta
     2221 cggggaatac cgcctggatg ccgctccggt ccgggtatgg gtggtccggg aagagctgtt
     2281 cctgaccccg gaatgggagg agatcccctt ccggtcaccc ccgggaaccc ggatccgggc
     2341 ctattcccgg accgtaggga cccgggaggc gccggatacg gccttcgctc tccgggattc
     2401 cgcctactgg atcctggcgg aggtacccgc ggcgctttcg aatccggcgg ccttcctgga
     2461 agccctcgcg gcgcgtctag cctacttcgc ggcccaggcc aagtccccgg gggacctttc
     2521 cctgcccgcg gtgctggagt atcgtccctg agggcggtcg agcggttttc ggccgccccg
     2581 actccgttcc atccgtgttt ctacaaagaa aatcaccttt tttatattga atccttcccg
     2641 acatggctat atactggtgc ggcccgtccg tagggacgga agcgcttaaa cgtcagggtt
     2701 cttagaggta tgttcaagga ggcttcatga acttccggaa agctgcgttc gtcgttctgg
     2761 cgatcgcgtt ggttcttgcc ggctgcggcg gcaagcagaa ggccgacacc atcaaggttg
     2821 gatggttcgg agccctcacc ggcgaccagg ccgtgtgggg agagaacgag ttcaacaccg
     2881 tcaagatgct cttcgaggag tacaacgcca agggcggcat cgaggtgggc ggcaagaagt
     2941 acatgctcga ggccatcggc tacgacaaca agggtgacgc ccaggaagcc gtgaacgtca
     3001 cgaaccgcct ggtctcccag gacaaggtcg tggccatcct cggccccaac gcctcgggca
     3061 acgccatccc catcgcctcc accctggagc aggccaaggt tccggacatc gccactgtgg
     3121 cgaccaaccc caaggtcacg gtccaggacg gcaaggtgaa gccctacaac ttccgcgtct
     3181 gcttcatcga cccctaccag ggcgccgtgg ccgccggcta cgccttcgac aagctcggcg
     3241 ccaagaaggc cgccgtcctc tacgacgtgt ccgacgacta ctcccagggc ctcaccgagt
     3301 tcttcgtgaa gtccttcgag ggcaagggcg ggaagatcgt cgccaaggaa ggcttcaccg
     3361 ccggggacaa ggatttccgc gcccagctgt ccaagatcaa ggccgccgct cccgacgtca
     3421 tcttcatgcc ctacttcttc accgaggtgg ccctgtccgc cacccaggcc cgggagctcg
     3481 gcatcaccgc caccttcatg ggcggcgacg gatggccttc acccgacctg ttcaagctcg
     3541 gcggcaaggc cgtcgagggc gggatcttcg tgaaccacct cgactacgac cagcccacgg
     3601 tccaggacta caaggcccgc tacaaggcca agtacggcaa ggagaccgag ctgaacggct
     3661 acctggtcca cgacgcggtc ctcatgctgg tcgacggcct ccagcgcgcc ggcaaggtgg
     3721 acggagaggc cctggccaag gccttcgaga gcacggacat ccagggtatc accgggcgca
     3781 tcaagatcag cccgaccacc cacaacccag agggcaagga cgccgcgatc atcaagatcg
     3841 tggacggcca gtacaagttc ctcgagaact acgccgccca gtaagcggca gacccggggg
     3901 ccggttccgt cggacagacc gaggggccgg ccctcttcct atccattgag aacgggggcg
     3961 ccatgactca attcctccaa cagctcacga acggactttc catcggttcc gtgtacgcgc
     4021 tgatggccgt cggatactct ctcgtgtaca gcatcatgaa cttctccaac ttcgcccacg
     4081 ggggagtgat catgctgggc gcctatttcg gattcttcgg cctcaccacc ttcaagcttc
     4141 ccttcgcggc cgccttcgcc ctggccacgg ccggcaccgc cctggtcgcc atcgtcgtcg
     4201 agcgggtggc ctacaagccc ctgcgggacc gcaaggcccc cttcctgtac ttcatcatct
     4261 ccgcgatggg cgcctccatg ttcctggaga acttcgtcat cgcgacgatc gggccgaatc
     4321 ccaagcccta tccgcccatc atcccccggg agcccatcgc cctgggcgac ctggctctgg
     4381 gccgcctcga cctgatcatg ttcatcgtct cagcggcctg cctgaccctc ctggtgctgt
     4441 tcatcgaccg cacccgcctg ggcaaggcca tccaggcctc cgcatacaac atgaaagcaa
     4501 gcgccctcat gggcatcaat acggatagcg tcgtgatcct ggtcttcgcc ctgggaggct
     4561 tcctggcggg cgtcgccggg gtcctctacg gcatgaagta catggtctac ccgaccatcg
     4621 gggccatcac cctcaagtcc ttcatagccg ccgtcttcgg cggactcggc agcctgccgg
     4681 gcgccgtcct gggctccgtc ctcctgggag tcatcgagac cctggtggcg gggtaccttt
     4741 cgtcgcagta ccgcgacctc atcgccttcg tcatcctgat cggggtcctg atcgtgcggc
     4801 ccacgggcat catgggcaag gccgtcgagg acaaggcata gggggaggca tggacagtta
     4861 catcctgggc atcctgatgc tcgcgggcat caacctcgtg gccgtgctgg gtctctccct
     4921 gctcacgggc ttcacggggc tcttctcctt cggccacgcg ggcttcatgg ccatcggcgc
     4981 ctacgtcggg gccttcctca cggtgtcccc ggcccgggac gtgcccggcc tgggcctgcc
     5041 cctggtggtc ggcatcctgg gcggcggggc cgccgcgggc atcgtcagct ggctcgtggg
     5101 ccgcatcacc ctgaacctca agggcgacta cttctgcatc gccaccctgg gcttcgggga
     5161 ggccatccgc ctcgtgttca acaacgtcca gcgcttcggc ggatcccggg gctggcccct
     5221 gggcgccacc cggaatcccc aggacatctg ggtcatcctc ctggtggacg tcgtcgcgat
     5281 cctcctggtg gccaacctgg tccggtcccg gcacggccgg aacatgatcg ccatccggga
     5341 ggaggagctg gccgcccagg tgacggggat caacgtcttc cggtacaaga tggtcagcct
     5401 cgtgaccagc gccgtgctgg cgggggtcgc gggggtcctg ctgggggact acctcacctt
     5461 catcaacccc aagatcttca acctgaccaa gtccacggag ctcatcatca tcgtgatctt
     5521 cgggggcctg ggatccatat ccggctccgt cctgggggcc ctgctcctga ccgccctgcc
     5581 cgaggtcctg cgcttcttcg agatctggcg cctggtgttc tacggagccg cggtcatcct
     5641 gatcatgatc agccggcccc agggcctcct gggcggcatg gagctgactc ccgcggggat
     5701 ccgcaagatc ctggcggagc ggagggcccg gcaggccctg agggcggaag ccgcccggga
     5761 agccgaaggg ggctcgcgat gagcggcatc ctggaggtga agggcctgac caagcgcttc
     5821 ggcggattgg ccgcggtgga caacgtgagt ttctcggtcc gggagggcgg gatctccgga
     5881 ctcatcggac cgaacggggc cggcaagacg acgatcttca acctgatcac cggggtgtac
     5941 aaggtgaccg aggggtccgt ctccttctcg ggctccgaga tcacgaacat ggagcccttc
     6001 cggatcgcgg accgggggat cacccggacc ttccagaaca tccggctctt caagaacctg
     6061 acggtgtacg agaacgtcct gaccgcctgc cacatgagcg cgaagtactc cattcccgag
     6121 gccctcctgc gcctgccccg gtacctgcgg gaggagaagg ccctccgcgc caaggcggag
     6181 gacctcctat ccatcatggg cctcacggag cggcgggacg tccaggccaa caacctgccc
     6241 tacgggctcc agcggcgact ggagatcgtc cgcgccctgg ccctggatcc caagctcctc
     6301 ctgctggacg agcccgcggc ggggatgaac cccgacgaga cggagcagct catgcgcctg
     6361 atctccgaga tccggacgcg tttcaagctc acggtcctgg tgatcgagca ccacatggac
     6421 ctggtgatgc ggctctgcga aagcatcgtg gtgctcaact tcgggcagaa gctggcggag
     6481 ggcaccgccg acgagatccg gggcgatccg cgggtcgtgg aagcctacct gggatcggga
     6541 gggaactgac atgctgacgg tgcgagacat ccacgtatcc tacggcggca tccgggccct
     6601 gaagggcgtc tccctccggg tggagaaggg cgagatcgtc acgatcatcg gggcgaacgg
     6661 ggccgggaag tcgaccctcc tgaacgccat ctctgggttc ctgaagctcg gttccgggga
     6721 gatcctctac caggacaagc tcctgcccaa gcggccggac cgggtggtca aggccgggat
     6781 ctgccacgtc cccgaagggc gactggtgtt cgccaacctg accgtggagg acaacctccg
     6841 catgggggcc tacctgcgcc gggacaaggg ggcgatcgcc gcggacctgg acaaggtcta
     6901 cggcctcttc ccccgtctga aggagcggca gcgccagata gcgggcaccc tctccggggg
     6961 ggagcagcag atgctggcca tgggccgggc cctcatgacc gacggggacc tggttctcat
     7021 ggacgaaccc tccctgggct tggcgcccct cctcgtgcag acggtcttcg acatcatcct
     7081 tgagatccgg gccctgggaa agaccattct tttggtggag cagaacgcct acaaggccct
     7141 ggacgccgcg gaccgggcct acgtcctgga gcagggccgc atcgtgaaaa agggtcccgc
     7201 cgccgaggtc aaggcggatc cctccgtccg cgccgcctac ctggggaccg agagcaatac
     7261 ggccggctaa cgagcggggc aaagccccgc tcgttggacg aggccttgac cccgggcggg
     7321 caaatcccgt atagactccc atgacgttca actgggcggc cttcctctcc tatatcgtcc
     7381 tgaccaccta caccccgggg ccgaacaacc tcatgtccat gaacaacgcg gcccggttcg
     7441 gcctgcgcaa gggatttccc ttcaacttgg ggatctggtc cgccttctcc ctggtgatga
     7501 tcctctgcgc ggtcttctcc gcggccctgt acgctgtgat ccccaggatc cagctgccca
     7561 tgaagatcct gggcgcggcc tacatgctgt acctggcctg gaataccctg aagcccgctc
     7621 cccgcggctc cgaccgggag gccaagggcg ggtattggta cggcttcgcc ctgcagttcg
     7681 tgaatcccaa gatcttcatc tacgggatca cggcgatgtc ctccttcatc ctgcccaatt
     7741 tctcggcccc ggcgcaggtg gtcgccttcg ccctgctcct ggccttcgtg ggcttcacgg
     7801 gaaacatgct ctgggccctc ttcggtgccc tcttcctcaa gctgttctcc cgccactcgg
     7861 gggtggtccg ggcggtcatg acggtccttc tggtctactg cgcggtgtcc ttgtttctgt
     7921 agcccgcgcc gcgggccgga agtctactcc ccgtccagct cgctctcgtg cacccagacg
     7981 ccgcgccgga gggggcggga gaacacctcg aaccagcctc cttcggagga atccacggga
     8041 ttgaggtagc gtcccggggg atagcgctcc agcacggcgg aatcaaggga gggcgaggct
     8101 cggcccgcgg cccctctcgg ccccgcggaa acgccccggt acaggaccgg caccagggcg
     8161 tccaggtccg cccggtcgga ctcggccagg aaggtacgta cgccctcgag aaggatcccc
     8221 gcgtagaggg aggggttgtc gcgcatccgc gcccggtctt cgggattgcc caggaagccc
     8281 atctccacca ggaccgcggg cgtgtagggg gacagggtgt tggagaacct ccaccaggag
     8341 aagccgaagt acccgcgcat gttcgccgtg acgccgccct ggtcctcggg caggccggag
     8401 gcgcggaagg cgtcggccat cgcctcggcg agccgccggg attccgggct cggcctccaa
     8461 ggcggggcga gtttccagcc cctccggtcg ggccggtccg cccggtccgc gtgcaccgag
     8521 acgaagaggt ccgcccggta ccccgcgggc acggcggcgg gcaccagctc cgccgagtag
     8581 cccgcggcgt tcaggcggtc gacgaggatg cgggagaccg cgaggttcag gtccagttcc
     8641 cggagggagc cgaaggccgc gcccgtggag gtccggagcc gggcaagttc gtccgggagc
     8701 tcgtcgatcc tccagtgtcc gggctgtatc ccgatcttga taggcccttc cagggcttcc
     8761 agggagtagc cgggaaggcc ccgaaggggt tccgggactc cgaccgcggc catcgcggag
     8821 gcgtccctcg cccgagcgaa gcccgacaat ccgatcgcgg cgattccgag gacggacgcc
     8881 gcaaggagga cggggaccag gatccgaagg atcggacggt cgccgtcagg cctcggcgag
     8941 cggcccggga tatcggaatt cataccaaaa taataaacat atatccctgt ccgttacaag
     9001 aaatgatgaa cggggctcct cggtaggcgc ctcgctcatc gagaggtaat tattgtcgtc
     9061 tgcgggaccc ctggtccttg tggtgagccg gttccgggct tggcccggaa ccgtatagcg
     9121 aaccgcgcgt cagcgcggtt cgctggaatc cactttctct actatcttta tgtgtaactc
     9181 gttgagctgc ttctgatcca cctcgttggg gcaatcgtgc gccgggcctg gcggcccggc
     9241 gctcatcgag aggaagtgat tatcgtgtgc ggcacccgct gcgcgggatg ccgcacgacg
     9301 attaccatcg gcctacttgg attccgcctt ctccacaatc tttatgtgca attcgtccaa
     9361 ctgtttctgg tccacctcat tggggcaatc atgcgaggcg cctcggggag gcgcctcgct
     9421 catcgagagg aagtgattat cgtgtgcggc caaaggccgc acg
//
LOCUS       DAOTEI010000139         9438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5163_length_9438_cov_4.413834, whole genome shotgun sequence.
ACCESSION   DAOTEI010000139 DAOTEI010000000
VERSION     DAOTEI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5163_length_9438_cov_4.413834"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="P5313_12450"
     CDS             complement(<1..727)
                     /locus_tag="P5313_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRY81222.1"
                     /translation="MRPFKVVAPYGPAGDQGQAIEALAKGVRAGQRYQTLKGVTGSGK
                     TFTMAKVIEEVQLPTLVISHNKTLSAQLYREFKDFFPDNAVEYFVSYYDYYQPEAYVP
                     TRDLYIEKDADINQEIDRLRLAATAALMERRDVIIVATVSCIYGLGSPDLYRAMRVYI
                     DRGSEIDLDDVKRRLVSLQYERNDAVLERGRFRVRGDTLEIHPAYANEAYRIELDFDR
                     VARIRKFDPISGAVVADDLEEAVV"
     gene            complement(1098..2357)
                     /locus_tag="P5313_12455"
     CDS             complement(1098..2357)
                     /locus_tag="P5313_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY81223.1"
                     /translation="MRLYSRGQVLLFSVCSFAVALLAAFGLGFIRLPAAAAASEGAAP
                     AALERFDAGAPAPSLEPGVISSPHLLTADTSQDYSQDERENISVYERLNSGVVNITTE
                     VVSINYFLEPVPSAGGSGSGSIIDPAGYVLTNYHVVKDAFKVYVNLSDGSRFEGKVQG
                     TDEENDLAVLKFDPPRDRRLTVIPYGDSSGLRVGQKVLAIGNPFGLERTLTRGIVSGI
                     GRPVQQDQKTIIRDMIQTDASINPGNSGGPLLNSRGEMIGINTMIYSPSGGSVGVGFA
                     VPVNTAKRVVPDLIRFGMVKRGWIDITAVQLFPALVEFMKERGYPVPVEQGLLVSQAR
                     RNGNADRAGIRGGSTPARYYSSVFYVGGDIITAIAGKKVASIADLYSALEDTKPGQEV
                     DVEYYRSGRKLTVKLDLSDRAEYRKQE"
     gene            2454..3521
                     /locus_tag="P5313_12460"
     CDS             2454..3521
                     /locus_tag="P5313_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRY81224.1"
                     /translation="MPSQIAHALVAEAALARTDPDEARRIAAGALGPCFRLGCQGPDI
                     FYHSQRTRPLALHYGVLAHRRGFGRLVEGMLETWIREDGDPASSWAAFLLGFATHGAL
                     DRAAHPYIVHGSGWTEPGKPETERFRGCHAFLERILDILLWEGRTGTPVSGFDVRVRL
                     LPAGEFPPEFPARLTSAFRRAYPSETAGDPRLTARFANALADTRRFLLLTNPAETSRR
                     AEGSGHDPESLRHLRGPHGRRIVSVLYPETFDRGVDWASERGRTWVHPCRGEPVRRDS
                     FFDLCAAAETEAAGLLRLVLEGIRNRSVPQGLADAAGNGTLNVGDAEGRPARPRFQDP
                     FPLAEAMSEELRSRLSLEASH"
     gene            3557..4744
                     /locus_tag="P5313_12465"
     CDS             3557..4744
                     /locus_tag="P5313_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRY81225.1"
                     /translation="MNMKDRLEKAYGGKKDPSSPAPRGPGAPDKPKPGAAKGKPASKG
                     GTVSPTRPPADFGVEKTEALLKTGVPGIEKAAKFLLLLGQEEAAKVIRHMKPDEIERV
                     SRAIAGIDRIDTIEANEILTEFGWLVKTQGASIEGGAETAQRMLAAAFGDDKARELLR
                     KAVPESAKPFQFLNDFDPKELVALLRNESPEVMSVLLPYLEPRLASAVIVSLADPVRT
                     EVVRRIARLEKVDPEVLRRMEEGIKEKIRRIGHSATEEVDGRSALANILRHVDTDLEE
                     SILGSLEEDNRDLSDSIRERLFTLDDILRVPDREVQKRLRDLSEREIALVLKGKSAAL
                     RDKLFQNVSQTRRLIIQEEYEILGAQRREDVERGTRDFLAWFKQRWDDGELSLEGDED
                     LVD"
     gene            4748..5281
                     /locus_tag="P5313_12470"
     CDS             4748..5281
                     /locus_tag="P5313_12470"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRY81226.1"
                     /translation="MSTISTKTGDGGLTDLWSGERVRKDDARVEAYGTVDELSSFLGE
                     SKHWVKRPETLAILEEIQRDLFRAAGELASRGTPFTDPVLPRDEERLFAYVERFEGEL
                     GLKGFVVPGSTVQSAKLDIARTVARRAERRIVTLARDSEVSPALRKYVNRLSDLLFVL
                     ARWEEAGEDRIIYVNNK"
     gene            5449..8394
                     /locus_tag="P5313_12475"
     CDS             5449..8394
                     /locus_tag="P5313_12475"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRY81227.1"
                     /translation="MTTYHSAFETLSEFPVPEYRAFGIHLRHRATGCEVFHLRSEDEE
                     NTFAFCFRTPSLDGTGVAHIVEHSVLCGSERFPVKDAFISLARGSLATFLNALTYPDK
                     TVYPAASTVEADYFNLMDVYGDAVFHPRITPDTFLQEAHHLEYGSDGKLDRKGVVYNE
                     MRGDYSSPDSLIATRTYETVFSPGHPYHADSGGDPDRIPNLSYEAFRSFWERNYHPSN
                     CRIFLHGSIPTERQLAFLEERFLSGYSRREPDTEIPLQPRFSGPVRASFPFPAEEGTE
                     ARTTTLVTWLTAAPEESVDNLTLEVLGEVLMGSDGAPLAHALTESGLGEDLSPHSGLD
                     TGLRQALFSAGLRGVERGRESEIEDLVLRTVGDLVRDGIPRDLLDAAFHSIEFSSREI
                     RRGGGTYGLRLLSRSLRGWLHGKTPDRTLSFETPLAELRRRMEADPRYLEEKLREWIL
                     DNPHRATVTVYPDPALAEARRRERRASLDALERGLSEAEKSDIRKTAEALRESQDAED
                     GAGTRETIPLLRRSDIPQEIEIIPRESTKIAGAAASLHPVFTNGIVYADFAVPLSGLP
                     ESRLCLLPLLARFVSGAGLRGRSYAEVSRDLARTAGGFHVLLQAATPAGNPADIPSVW
                     MVFRLKALAGKLPAALDLVLPLMTEPDFSDLKRLSDLLSELANDMTSAIIPSGSSFAA
                     ARAAASFSRAQGIEELWRGLSQFLFLSDLRADTKTETLAGRLSDLLRSTVGREALRVN
                     LTADPETLESARSALESALSRLPVRPAGAAAPPLAEAPAPWTAPPARFEAWSLPAQVG
                     FSAACLRASPIGTPGFAHEQALGHLLSTGYLWDEVRVKGGAYGASAWTDGSESTFSFS
                     SYRDPAPAATRAAYRGALESVAGDGLARDVLERAVVGAAGRDLRPMMPEEKGFIDFKR
                     ELFGVTDEMRRRKRAELLALDREDLRAAAGRILSGWEDRTEVLISHEDDIESLKRENP
                     AFSVRQLQL"
     gene            8408..8602
                     /locus_tag="P5313_12480"
     CDS             8408..8602
                     /locus_tag="P5313_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81228.1"
                     /translation="MRDEGFNVLKSLASELQGAPFPKTVTKELYTIWYEHAQRIAQDA
                     LEYLAENAPDYGPETQDLDI"
     gene            8610..9359
                     /locus_tag="P5313_12485"
     CDS             8610..9359
                     /locus_tag="P5313_12485"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRY81229.1"
                     /translation="MSIPLRELTYGPCPYLAQAPSWKVAEFSVRELPPAVYENLLVRG
                     FRRSGTSFYRTICEGCDRCIPIRIDVERFTPTKSQRRVARLNSRTGFAFDGAGGGFRE
                     DRFRLYEAYCRDRHGKTHAAEDSALSYFAFLVDSPLGSVAVTEYSTPDGEGRSLAGNG
                     YLDVLPDGLSSVYFAWDARVHRLSLGIYSVLREIELCRNLGKRWYYLGFWVPGSRVMD
                     YKANFEPAEIAREGAWIPLERTLRDILRGAA"
BASE COUNT         1570 a         3249 c         3053 g         1566 t
ORIGIN      
        1 agacgacggc ctcctccagg tcgtccgcga ccacggcccc ggagatcggg tcgaacttgc
       61 ggatccgggc cacccggtcg aaatccagtt cgatgcggta ggcctcgttg gcgtaggccg
      121 ggtggatctc cagggtgtcc ccccggaccc ggaaccgtcc ccgctccagg acagcgtcgt
      181 tccgttcgta ctggagggac acgagtcggc gcttcacgtc gtccaggtcg atctcggagc
      241 cccggtcgat gtagactcgc atggcccggt agaggtccgg actccccagg ccgtagatgc
      301 aggagaccgt ggccacgatg atcacgtccc ggcgctccat gagggccgcc gtcgccgcca
      361 ggcggaggcg gtcgatctcc tggttgatgt ccgcgtcctt ctcgatgtag aggtcgcggg
      421 tgggaacgta ggcctcgggc tggtagtagt cgtagtagga gacgaagtat tccaccgcgt
      481 tgtccgggaa gaagtccttg aactcccggt agagctgggc cgataaagtc ttgttgtggg
      541 agatgaccag ggtgggaagc tggacctcct cgatgacctt ggccatggtg aaagtcttgc
      601 cggagcccgt cacccccttc agggtctggt agcgctgtcc ggcccggact cccttggcca
      661 gggcctcgat agcctggccc tgatcgcccg cggggccgta gggcgcgact accttaaacg
      721 gtctcatgga tcctctcaca cggccccgcg gtcggatcgc ggggaatcgg attgtacggc
      781 gccgcttccg cggcgtgcga tacgcctgca ggcccatatg gtgccactac tttgaacggt
      841 ttcatgtatt aggctctccg atcaccgtgc ccgattgaac gaggtctgga acgttaatcc
      901 aatccatttc ccgaaaaaag cgaagcagga tgatcatgat gtacaagatg aacaagatct
      961 aaaaacgaga gtacgatcca agatcatcct tttcatcctt tcccgatcct gtccatcctg
     1021 tcccgtccaa ggtttatcgt cccactccgt gctctccgtg agctccgtgg tgagtcctgg
     1081 aatgtacgcc tcgcggccta ttcctgcttc cggtactcgg cgcggtccga gaggtccagc
     1141 ttgacggtca gcttccgtcc ggagcggtag tactccacgt cgacctcctg cccgggcttg
     1201 gtgtcctcca gggccgagta gagatccgcg atgctggcga ccttctttcc cgcgatggcg
     1261 gtgatgatgt ccccgcccac gtagaagacg ctggagtagt agcgggcggg tgtggaaccg
     1321 ccccggatgc cggcccgatc cgcgttcccg ttccttcgcg cctgggaaac caggagtccc
     1381 tgctccacgg gcacgggata cccccgttcc ttcatgaact ccaccagggc ggggaagagc
     1441 tggaccgcgg ttatgtcgat ccagccccgc ttgaccattc cgaaccggat caggtccggc
     1501 acgacccgct tggcggtgtt cacggggacg gcgaacccga cccccacgga cccgccggag
     1561 ggggaataga tcatggtgtt gatgccgatc atctcccccc gggagttgag gaggggaccg
     1621 ccggagttgc cggggttgat ggaggcgtcc gtctggatca tgtcccggat gatggtcttc
     1681 tggtcctgct ggaccgggcg accgatcccg gagacgatac cccgggtcag cgtgcgctcc
     1741 aggccgaagg ggttgccgat ggccaggacc ttctgcccga cccggagccc cgaggagtcc
     1801 ccgtagggga tgaccgtcag ccgccggtcc ctggggggat cgaacttgag gaccgccagg
     1861 tcattctcct cgtcggtgcc ctggaccttg ccctcgaagc ggctgccgtc ggaaaggttc
     1921 acgtagacct tgaaggcgtc cttgaccacg tggtagttgg tcaggacgta gcccgcggga
     1981 tcgatgatgg acccggaacc cgagcctccc gcgctgggca cgggctccag gaagtagttg
     2041 atggagacga cttcggtggt gatgttgacc accccggagt taaggcgctc gtagacgctg
     2101 atgttctccc gctcgtcctg ggaatagtcc tgggaggtat cggcggtcag aaggtggggg
     2161 gaagagatga ccccgggctc cagcgacggg gccggggctc cggcgtcgaa ccgctccagg
     2221 gccgcggggg cggcgccctc cgaggccgcc gcggcggccg gcaggcgtat gaatccgagg
     2281 ccgaaggccg caagcaaggc tacggcgaag gaacagaccg agaacaacag cacctgtccg
     2341 cgggaataga gcctcatacg catctccttg ccatttcatg atacttgttt tccggaaaaa
     2401 agccaacgaa gccgcttcca aggaaggcgg atcccggagt attttcaagg tatatgccct
     2461 cccagatcgc ccacgccctg gtcgcggaag cggccctggc ccggacggat cccgacgagg
     2521 cccggagaat cgccgccgga gccctcggtc cctgtttccg cctgggctgc cagggcccgg
     2581 acatattcta tcacagccag aggacccgtc ccctggccct ccactacgga gtactggccc
     2641 accggagggg tttcggccgg ctcgtggagg gcatgctgga aacctggatc cgggaggacg
     2701 gggatcccgc ctcctcctgg gccgccttcc tcctgggctt cgcgacccac ggcgccctgg
     2761 accgggctgc ccacccctac atagtccacg gctccggctg gaccgagccc gggaagcccg
     2821 aaacggaacg gttccggggc tgccatgcct tcctggaacg gatcctggac atcctgctct
     2881 gggaagggcg gacggggacc ccggtgtccg gcttcgacgt ccgggtccgc ctcctgcccg
     2941 cgggggagtt tcctccggaa ttcccggccc gcctgacctc ggccttccgc cgcgcctacc
     3001 cctcggagac ggccggggat ccccgcctga ccgcccggtt tgctaacgcc ctggccgaca
     3061 cccggcgctt cctgttgctc acgaatcccg cggagacttc ccggagggcg gagggttccg
     3121 gccacgaccc ggaatccctg cggcacctcc ggggccccca cggaaggagg atcgtctccg
     3181 tgctctaccc ggaaaccttc gaccggggcg tggactgggc ctcggagcgg ggacggacct
     3241 gggtccaccc ctgccggggg gagcctgtgc gccgggatag cttcttcgat ctctgcgcgg
     3301 ccgcggaaac cgaggctgcg ggcctgctca ggctcgtcct ggaaggcatc cggaaccggt
     3361 ccgtgccgca gggcctggcg gacgccgcgg ggaacggcac cctgaacgtc ggggatgccg
     3421 agggacggcc ggcccggccc cggttccagg atccctttcc cctggccgag gcgatgtcgg
     3481 aggaactgcg ctcacggctc tcccttgaag cttcgcattg accgcccgta aagccccgga
     3541 tagtatagac ctcgatatga acatgaaaga caggctggaa aaggcgtacg gcgggaagaa
     3601 ggatccttct tcccctgctc cccgaggtcc gggcgccccc gacaagccca aacccggtgc
     3661 cgcgaagggc aaacccgcct cgaagggcgg gaccgtctcc cctacccggc cgcccgcgga
     3721 cttcggggtg gaaaagaccg aggccctcct caagaccggg gtgcccggca tcgagaaggc
     3781 cgccaagttc ctgctgctcc tgggccagga ggaagccgcc aaggtcatcc gccacatgaa
     3841 acccgacgag atcgagcggg tctcccgggc catcgccggg atcgaccgga tcgacaccat
     3901 cgaggccaac gagatcctca cggaattcgg ctggctggtg aagacccagg gggcctccat
     3961 cgaggggggc gccgagacgg cccagcgcat gctggcggcc gccttcgggg acgacaaggc
     4021 ccgggagctc ctgcgcaagg cggtccccga gtccgccaaa cccttccagt tcctgaacga
     4081 cttcgacccc aaggaactcg tggccctcct gcggaacgag tccccggagg tgatgagcgt
     4141 cctcctgccc tacctggagc cccggctggc cagcgcggtg atcgtgagcc tggcggatcc
     4201 cgtgcgcacc gaggtggtcc gacgcatcgc ccggctggag aaggtggatc ccgaggtcct
     4261 ccgccgcatg gaggagggca tcaaggagaa gatacggcgc atcggacatt ccgccaccga
     4321 ggaggtggac ggccgctcgg ccctggccaa catcctgagg catgtggaca cggacctgga
     4381 ggagagcatc ctgggcagcc tggaggagga caaccgtgac ctgtccgact ccatccggga
     4441 acggctcttc accctggacg acatactgcg ggtgccggac cgggaggtgc agaaacgcct
     4501 gcgggacctc tcggagcggg agatcgccct ggtcctcaag ggcaagagcg cggccctgcg
     4561 ggacaaactc ttccaaaacg tatcccagac ccgcaggctc atcatccagg aggagtacga
     4621 gatcctgggg gcccagcgcc gggaggacgt ggagcgaggg acccgggatt ttttggcctg
     4681 gttcaagcag cgctgggacg acggagagct ctccctggaa ggggacgagg acctggtgga
     4741 ctaggccatg agcaccatct cgaccaagac cggcgacggg ggactcacgg acctgtggtc
     4801 cggggagcgc gtccgtaagg acgacgcccg cgtggaggcc tacgggaccg tggacgagct
     4861 gtcgagcttc ctgggggagt ccaagcactg ggtcaagcgg ccggagaccc tggccatcct
     4921 cgaggagatc cagagggacc tcttccgcgc cgccggggaa ctggcttccc gggggacacc
     4981 cttcacggac cccgtccttc cccgggacga ggaacgcctg ttcgcctacg tggaacggtt
     5041 cgagggcgaa ctgggcctca agggcttcgt cgtgcccggg tccaccgtgc agtccgccaa
     5101 gctcgacata gcccgcaccg tagcccgcag ggcggaacgc cgcatcgtca ccctggcccg
     5161 ggactccgag gtctcaccgg cccttcggaa gtacgtgaac cgcctgtccg acctcctctt
     5221 cgtcctggcc cgctgggagg aagccgggga agatagaatc atctacgtaa ataataaata
     5281 gcagacatat caggagctca ccacggagga cacggagacc acggaggaga aaggagagat
     5341 cttgaattct ctttcccctg ttctttctcc gtgacctccg tgagctccgt ggttcattcc
     5401 tgaaattcat gtcttgactc tctaccgtcc ggcggagatc ttgtacccat gaccacctac
     5461 cactccgcct tcgagaccct ctccgagttt cccgtgcccg aataccgcgc cttcgggatc
     5521 cacctgcggc accgcgcgac cggctgcgag gtcttccacc tgcggtccga ggacgaggag
     5581 aacaccttcg cgttctgctt ccgcaccccc agtctcgacg gcaccggggt ggcccatatc
     5641 gtggagcact ccgtgctctg cggctccgag cgctttcccg tcaaggacgc cttcatctcc
     5701 ctggcccggg gcagtctggc caccttcctg aacgccctga cctatccgga caagaccgtg
     5761 taccccgcgg cgtccacggt ggaggcggac tacttcaacc tgatggacgt gtacggggac
     5821 gcggtcttcc acccccggat caccccggac acattcctcc aggaggccca ccacctggag
     5881 tacggatccg acggaaagct ggaccgcaag ggtgtggtct acaacgagat gcggggcgac
     5941 tattccagcc cggacagcct gatcgcgacc cggacctacg aaaccgtctt ttccccgggc
     6001 cacccctacc acgcggactc cggcggagac cccgaccgga tcccgaacct cagctacgag
     6061 gccttccgtt ccttctggga acggaactac catccctcca actgccggat cttcctgcac
     6121 ggttcgatcc cgacggagcg ccagctggcc ttcctggagg aacggttcct ctccgggtat
     6181 tcccggaggg agccggacac ggaaatcccc ctccagcccc ggttctccgg accggtccgg
     6241 gcatccttcc ccttccccgc cgaggagggc accgaggccc gcacgacgac cctggtcact
     6301 tggctgaccg cagcgcccga ggagagcgtg gacaacctga ccctcgaggt cctgggggag
     6361 gtactcatgg ggtccgacgg ggcccccttg gcccacgccc tcacggaatc cgggctcggg
     6421 gaggacctgt ccccccacag cggcctggac acgggcctgc gccaggccct cttttccgcg
     6481 ggtctccgcg gcgtcgagcg cggccgggaa tccgagatcg aggacctcgt cctccggacc
     6541 gtgggcgacc tggtacggga cgggatcccc cgggacctcc tggacgcggc cttccattcc
     6601 atcgagttct cctcccggga gatccgccgt ggagggggaa cctacggact ccggctcctg
     6661 tcccggtccc tccggggctg gctgcacggc aagaccccgg atcggaccct ctccttcgag
     6721 actcccctgg cggaactcag gcgacgcatg gaagccgatc cccggtatct cgaggaaaag
     6781 ctccgggaat ggatcctgga caatccccac cgcgccacgg tcacggtcta ccccgacccg
     6841 gccttggcgg aggcccggcg cagggaacga cgggcttccc tggatgccct ggagcggggc
     6901 ctttccgagg cggagaaatc ggacatccgg aagaccgcgg aagcccttcg ggaatcccag
     6961 gacgcggaag acggggccgg cacccgggaa acgatccctc tcctccggcg ctcggacata
     7021 ccccaggaaa tcgagatcat cccccgggag tccaccaaga tagccggggc ggcagcctcc
     7081 ctccatccgg tcttcaccaa cgggatcgtc tacgcggatt tcgccgttcc gctctcgggt
     7141 ctgccggaat cccggctctg cctccttccc ctcctggccc gtttcgtgtc cggggcgggg
     7201 cttcggggcc ggtcctatgc cgaggtctcc cgggacctgg cccggacggc gggcggattc
     7261 catgtcctgc tacaggccgc cacccccgcg gggaatcccg ccgacatccc ctccgtctgg
     7321 atggtcttcc gtctcaaggc cctggcgggc aagctccccg ccgccctgga cctggtcctg
     7381 cccctcatga cggagccgga tttctcggac ctcaagcggc tttccgacct cctttcggag
     7441 ctggccaacg acatgacctc cgccatcatc ccctccggga gctccttcgc cgcagcccgg
     7501 gcagcggcct ccttctcccg ggcccagggg atcgaggagc tctggcgggg cttgtcccag
     7561 ttccttttcc tctcggacct ccgggcggat acgaagacgg aaaccctggc cggccggctc
     7621 tccgacctgc tccggtccac ggtcggccgg gaagccctgc gggtcaacct gaccgcggat
     7681 ccggaaaccc tggaatccgc ccgctccgcc ctggagtcgg ccctttcccg cctgcccgtc
     7741 cggcccgccg gggcggcggc ccctcccctc gcggaggccc ccgccccgtg gacggccccg
     7801 ccggcccggt tcgaggcctg gtccctcccc gcccaggtcg ggttcagcgc ggcctgcctc
     7861 cgggcctccc cgatcgggac tccggggttc gcccacgagc aggccctggg tcacctcctg
     7921 tccacgggct acctctggga cgaggtccgc gtgaagggag gggcctacgg agcctccgcg
     7981 tggaccgacg ggtcggaatc caccttcagc ttctcctcct accgggatcc cgccccggcc
     8041 gccacccggg ccgcctaccg cggggccctg gaatccgtcg ccggagacgg cctggcccgg
     8101 gatgtcctgg aacgggccgt cgtgggggcg gcgggacggg acctccggcc catgatgccc
     8161 gaggagaagg gcttcatcga cttcaagcgg gaactcttcg gggtgaccga cgagatgcgc
     8221 cgacggaagc gtgcggagct cctggctctg gaccgggagg atctgcgggc cgccgccggc
     8281 cgcatccttt cgggttggga ggaccgaacc gaggtattga tttcccacga ggatgatata
     8341 gaatcgttga aacgggagaa tccggcattt tccgtacgcc aactccaact ctgaggagat
     8401 gcgcgaaatg cgcgacgaag gtttcaacgt gctcaagtcc ctggccagcg agctccaggg
     8461 ggctccgttt cccaagacgg tgacgaagga gctctatacc atctggtacg agcacgccca
     8521 gcgcatcgcc caggacgctt tggagtacct cgcggagaac gcccccgatt acggaccgga
     8581 aacccaggac ctggacatct aaggtgacca tgtccattcc cctccgggaa cttacctacg
     8641 ggccctgtcc ctacctggcc caggctccat cctggaaggt ggccgagttc tccgtccggg
     8701 aacttccgcc ggcggtgtat gagaacctcc tggtccgggg tttccggcgc tcgggcacgt
     8761 ccttctatcg gacgatctgc gagggctgcg atcgctgcat ccccatccgg atcgacgtcg
     8821 agcggttcac gcccacaaag tcccagcgca gggtggcacg gctgaactcc cggaccggat
     8881 tcgccttcga cggcgcgggc ggcggcttcc gggaagaccg gttccggctg tacgaggcgt
     8941 actgccggga ccgccacggc aagacccatg cggccgagga ctccgccctg tcctatttcg
     9001 ccttcctggt cgactcgccc ctgggatccg tggccgtgac ggagtactcg acgcccgacg
     9061 gggaaggccg gagcctggcg gggaacggat acctggacgt gcttcccgac gggctttcct
     9121 ccgtctattt cgcctgggac gcccgggtac accggctatc gctgggcatt tattccgtcc
     9181 tccgggagat cgagctctgt cggaacctcg gcaagcgctg gtactacctg ggtttctggg
     9241 tgcccggttc ccgtgtgatg gactacaagg ccaacttcga gcccgccgag atcgcccggg
     9301 agggcgcatg gatccccctg gagcggactc tccgggacat cctccggggg gccgcgtgaa
     9361 gtcgggaaag ggaccgaccg ccgagacgga tcggcggata ccggaatcgg accgcgcccg
     9421 gatcgacggc ctcaacga
//
LOCUS       DAOTEI010000140         9407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5179_length_9407_cov_5.217601, whole genome shotgun sequence.
ACCESSION   DAOTEI010000140 DAOTEI010000000
VERSION     DAOTEI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5179_length_9407_cov_5.217601"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="P5313_12490"
     CDS             <1..1544
                     /locus_tag="P5313_12490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY81230.1"
                     /translation="VFIFAFMVAGIYFRTKSAIETRETGLAGFYRDAVMLALQDWLAE
                     RESDIAWMASILESDAERAGTPLAPDAHDRFKALLEAKDSFSDALLLDLEGNVVFGRS
                     GPPARKVSLADRDYFQAAREGRAFVSGVFRNRLTGRYAFAVSRPLRYRGEIRGVLAGT
                     VLLADLVRIVESMGLGSLGSVYITDPERRLVAASSEADFRSPADTAEEGPVLDNLAVR
                     EAVARREGVAKYRGESGRTVIGAYGWCPRLNLGMVVELRSDQAYLPITRLLEFFAQFA
                     AGLLVLQLVLAYALSARLIRPIRALAEAAGTLEEREYPEVQAPRTNTELDNLVDTFNR
                     MAKAVRDREGLLKESAARDSLTGLYNHAKIEEFLDFEIRRSRRDDRPVCFVMMDIDHF
                     KSVNDTFGHQAGDTVLKETAGLILRSGRDGDIVGRYGGEEFAVILNARSSGEAAAYCE
                     RIRKAVEDTAFVHEGVEIRVTISLGYSCALPSALGPFELIRAADKALYAAKQAGRNRV
                     VAAAL"
     gene            1701..2264
                     /gene="efp"
                     /locus_tag="P5313_12495"
     CDS             1701..2264
                     /gene="efp"
                     /locus_tag="P5313_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRY81231.1"
                     /translation="MIRGGDIAKGSVLLINSHPHIVVDREFVNPGKGAAFARVKAKNM
                     RNGNVVTQTIKTQDEVEDAQVDLIDCQYQYSDAENYIFMNNESFEQYFIPFPGHEDKK
                     FYLQEGETYSILFYEGEAIDIKIPYKMVFTVAESENYVRGDTVSGATKPVVCETGLVV
                     RVPLFIKQGEKILVNTETNEYQERVNT"
     gene            2408..3631
                     /locus_tag="P5313_12500"
     CDS             2408..3631
                     /locus_tag="P5313_12500"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY81232.1"
                     /translation="MIEPFASPARLPSLDPYIHKSVSFRYSGRELRLDLSHALFSSFD
                     IDRGTRLLLKVLARSRFAEGARSLLDLGCGVGILGIALGSASPGSEIHFRDRDALAAA
                     FAARNARANGLEPASWGTGLTLAGLAGRRFDRIVSNVPAKAGPPVIEDFVRRLPSALS
                     EDGRFALVVVNPIAPAVRAAALASGAEIHEEEAGPGHTVIAGGRGTELPPAADELAVY
                     ERCRSAFELAGIRYPLSGYWGLPDFDTPSYAAVLAAGLCARAAGDAVIRRVSIGNPGP
                     GHLALYLCGRFGVERLRLVSRDCLQTAATRRNLAAAGFSPELSESDGLETGESFEGGF
                     DLIVEFPDPVPEFDWIGPIWERASRSAAPGSAFVMVGRPTEAGRFDRRKPSGWTRAFE
                     RKRDGFSGLVYRREG"
     gene            complement(3726..5801)
                     /gene="recG"
                     /locus_tag="P5313_12505"
     CDS             complement(3726..5801)
                     /gene="recG"
                     /locus_tag="P5313_12505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRY81233.1"
                     /translation="MYLRELTQSVSALKGAGPSVCARLARLGVYTVADLLLRAPRAYE
                     DRSRAVPFREALRTGLPANTVGVVTAHDWFGYGRTRTLKILVDDGTAVAALVCFNRAF
                     LADRHPVGSRSRVTGNLALRFNELQAASFEIEAAAEGQGPEAGVLPVYPLTDGLAQGT
                     VRKLAARALAEYGRRVEDEVPVRIRELRGLPPKARALEDLHAPGDPEAAEHALRALAY
                     EELFYLQVLVRRRAAARAERPADRSAGPPARAGLRPRLLERLPFRLTADQEKVAAEIA
                     ADMEKPRPMARLLQGDVGSGKTLVAFLAALKAVESGGQAALMAPTELLARQHAQTAAR
                     LLEPLGVRLAFLTGNLRDASRRPLLAALAAGEVDFVLGTHALFSEDVEYRRLALAIVD
                     EQHRFGVLQRLALSRKGGAPDLLMMTATPIPRTLALTVYGDLEVSTIRTMPPGRKPVV
                     THLARHGNEAKVYEFVRKELTEGRQAYFVYPLISGSEAGSLKDAEAMARHLAEEVYPG
                     IPGGLIHSRLPDEEKRSVMEAFTRGGLRYLVATSVVEVGVDVAAATCMVVEHAERFGL
                     AALHQLRGRVGRSEAQSYCFLVYSRDLSEEGKRRLKAMKETTDGFALAEEDLRIRGPG
                     EIAGIAQSGSLRLAFADPVRDADLLETARLDAAGIVDRDPGLLEPEHRVIREVLDRAN
                     PFPAESSGA"
     gene            complement(5824..6966)
                     /locus_tag="P5313_12510"
     CDS             complement(5824..6966)
                     /locus_tag="P5313_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81234.1"
                     /translation="MSPARTTARPTRRPSRTCPGAPAPQSFPAALRACLAALAAGALL
                     ASCATVQRDLTVERLPEDGYAVLDSHERDLVLLEAQPDRAALDALRSRLAETGSRPVL
                     ERTWSARLAALAGRAALLAQDRPVADRELRKALSLNPADEVARVLEIRLERNADKRKA
                     LLEEALKTAETQVRLRAELGRILLAEGRYAEALAAFDASLPFLPEPYEAVYGAERRRA
                     LALRDASAPAGSALLSDRPVSLQDLAALVQAESSLVDFLTGGKDWAPGILFERLRAGG
                     FYPDPAAPGTAAARRGEAAFLLWHLAANRRNDRSLLRRYSDRYRARPNPASPVPDVPL
                     EAPWFDSVLGCVEREIMELPDGRNFRPLDPVSGLEFYRWLRRAADL"
     gene            complement(6983..>9407)
                     /locus_tag="P5313_12515"
     CDS             complement(6983..>9407)
                     /locus_tag="P5313_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRY81235.1"
                     /translation="LAAPEAAGVRALVDALRRDPFLRSRAFRILADVRMDGALYGPAY
                     LAAVDLGIRSALTRTAPFWGPWLSIPGLTYSRSSGTSRFLYRAGDTVIHAAVRKNLLV
                     LAGDEGLLERALASGGAGRDAETAELLAGRAPRDLRILLRTAELLAGFRGDADPAGRF
                     LADLKLPGFAAVDLSLSNAAVQLTLELPVSSDRAALAALISARSRPPAVLSVLPESSR
                     YFSVLAAGSLEQVRDAALPLLGAEETWRQADAASKAALGMGLEDLLLSWSGDELGAFG
                     LSDRTEPVFFLKVRDEARRRRVFQEAFQSGTLGEDSAAVLDGIRVPRIVFPWYIRALL
                     DAAGADVPQPYFLVEDGYLLVSTSAESLVSAAREARAGKTLPRTDSWRRVSAGIPADA
                     AAMIYYSLDRSVPFFLRGTGPLAEFLRRYGEGTASIRFGDGRMKLALSAVALGGRGTR
                     DLPGFPLRTERRPVGGMEAGRGPDRRVRLYWMEEGGTLVSYLPASGIRKALSLDDSGH
                     FVLETLPDGTVAAVWAVSRRGTVWRLDPDLAASEGFPRATGARPAAAPTLAPDPEAKG
                     TSPALRLLVPLRDGGILTVSSSGTEGSLPVTFEDPVSVPISRWNGFLGIYPKSFDGTL
                     WILDSGGQALPGWPVALPGIGYGSPVLAPLGSSFRAAFLTQAGTLSCWNDRGEPVPGF
                     PVDLEGVFYGTPIFAADAYWAVSESGTLYRVGLDGRRTRTDVRGLGSREPVLAARDVD
                     GDGRPEIFVSGDGNALYGFDADLEPLTGFPVQGTGRPAFADLNGDGRPELVSAGVDGT
                     VRAVTFQAP"
BASE COUNT         1444 a         3248 c         3425 g         1290 t
ORIGIN      
        1 ccgtcttcat cttcgccttc atggtggcgg gaatctactt ccggaccaag tccgcaatcg
       61 agacccggga gacggggctt gcggggttct accgggacgc ggtcatgctg gctttgcagg
      121 attggctggc cgagcgggag agcgacatag catggatggc ctcgatcctg gaatccgacg
      181 cggagcgggc cgggaccccc ctggccccgg acgcgcacga ccgcttcaag gctctcctgg
      241 aagccaagga ctccttctcg gatgccctcc tcctggacct ggaggggaat gtggtgttcg
      301 ggcgatccgg ccccccggcc cggaaggtga gcctggcgga ccgggactac ttccaggcgg
      361 cccgggaggg aagggccttc gtctccgggg tcttccgcaa ccgcctgacg gggaggtacg
      421 ccttcgcggt cagccgtccc ctgcggtatc ggggggagat ccggggcgtc ctggcgggga
      481 cggtccttct ggccgacctg gtccgcatcg tggagagcat ggggctgggc agcctgggct
      541 ccgtctacat caccgatccg gagcggaggc tggtggccgc ctcgtccgag gcggatttcc
      601 gatcccccgc ggataccgcg gaggagggcc ccgtgctgga caacctcgcg gtacgggagg
      661 ccgtcgcccg gagggaggga gtcgccaagt accgcggcga gtcggggcgg accgtcatcg
      721 gagcctacgg ctggtgtccc cgcctgaacc tgggcatggt ggtggagctc cgcagcgacc
      781 aggcctacct gcccatcacc cgcctcctgg agttcttcgc ccagttcgcc gccgggctcc
      841 tggtgctcca gctcgtcctg gcctacgcct tgagcgcccg gctgatacgc cccatccggg
      901 ccctggccga agccgcggga accctggagg agcgggagta cccggaggtc caggctcccc
      961 ggacgaacac cgaactggac aacctcgtgg acaccttcaa ccggatggcc aaggcggtga
     1021 gggaccggga gggcctcctg aaggagagcg cggcccggga cagcctgacg ggcctctata
     1081 accacgcgaa gatcgaggag ttcctggact tcgagatccg gaggagccgg cgggacgacc
     1141 gtcccgtctg cttcgtcatg atggacatcg accacttcaa gtccgtcaac gacaccttcg
     1201 gacaccaggc cggggatacg gtcctgaagg agacggcggg gctgatcctc cgttccggcc
     1261 gggacggcga catcgtcggg cgctacgggg gggaggagtt cgcggtcatc ctgaacgccc
     1321 ggagttccgg ggaggctgcg gcctactgcg agcggatccg gaaggctgtg gaggataccg
     1381 ccttcgtgca cgaaggcgtc gagattaggg tcaccatcag tttggggtat tcctgcgcgc
     1441 tgccctcggc cctgggaccc ttcgaactga tccgggccgc ggacaaggcc ctgtacgcgg
     1501 ccaaacaggc cggcaggaac cgggtggtcg ccgcggccct ctagcaggag ccggctttac
     1561 aaaaccgggc gaatttgcca tagtgctgtg tccccgtgga atcccggcac cgagccgggg
     1621 accgtgggca gtccatccca tgtaaacccg gccccgtcct aattcccgca ggcccggagc
     1681 cgcaatccga gaggtacatc atgatccgag gcggagacat agccaagggc agcgtcctgc
     1741 tcatcaacag ccatccccac atcgtcgtgg accgggagtt cgtcaacccg ggcaaggggg
     1801 ccgccttcgc ccgggtcaag gccaagaaca tgcggaacgg caacgtggtc acccagacca
     1861 tcaagaccca ggacgaggtc gaggacgccc aggtcgacct gatcgactgc caataccagt
     1921 actcggacgc cgaaaactac atatttatga acaacgagtc cttcgagcag tatttcatcc
     1981 ccttcccggg ccacgaggac aagaagttct acctccagga gggggagacc tactcgatcc
     2041 tcttctacga gggcgaggcc atcgacatca agatccccta caagatggtc ttcaccgtgg
     2101 cggaaagcga gaactacgtc cggggcgaca ccgtctccgg ggcgaccaag cccgtggtct
     2161 gcgagacggg cctggtggtg cgggttcccc tgttcatcaa gcagggcgag aagatcctgg
     2221 tcaacaccga gaccaacgag taccaggaga gggtgaatac ttaggtgaat aggtgaatat
     2281 gtaaatgggt gaatggggtt ggacaggcag aaggcatgcc cggacatggc ttcgctcctc
     2341 aacctttcat tcccattcac ctattcacaa ttcaccaatt cacggaatac cgaaatagcc
     2401 cttggacatg attgaaccgt tcgcgtcccc cgcccgactc ccttccctcg acccctacat
     2461 ccataaatcc gtctccttcc gctattccgg ccgggagctc aggctggacc tgtcccacgc
     2521 cctgttcagc tccttcgaca tcgaccgggg aacgaggctc ctgctcaagg tcctggcacg
     2581 ctcgaggttc gcggagggcg cccggtcact cctggacctg ggctgcgggg tcgggatcct
     2641 ggggatcgcc ctgggctcgg cgtcgccggg ctcggagatc catttccggg accgggacgc
     2701 cctggccgcg gccttcgcgg cgcgcaacgc ccgggccaac ggccttgagc ccgcgtcctg
     2761 ggggacgggc ctgaccctgg ccggcctggc gggccgccgc ttcgaccgga tcgtctccaa
     2821 cgttccggcc aaggccgggc ccccggtcat cgaggacttc gtgcggcgcc tcccgtccgc
     2881 tctctccgag gacggccgtt tcgccctggt ggtggtgaat cccatcgccc cggcggtccg
     2941 ggcagcggcc ctggcctcgg gggcggagat ccatgaggag gaggcgggac ccgggcatac
     3001 ggtgatcgcc gggggccggg ggacggagct tccccccgcg gccgacgagc tggccgtgta
     3061 cgagcgctgc cgctcggcct tcgagctggc ggggatccgg taccctctgt ccgggtactg
     3121 ggggcttccg gacttcgata ccccctccta cgccgcggtc ctggccgcgg ggctctgcgc
     3181 ccgtgccgcc ggggatgccg tgatccggcg cgtgtcgatc gggaatcccg gaccgggcca
     3241 tctcgccctg tacctctgcg gaagattcgg ggtcgagaga ctccggctgg tctcccggga
     3301 ctgcctgcag accgccgcga cccgccggaa cctggccgcc gcgggctttt ccccggagct
     3361 ctcggaatcc gacggactgg aaaccgggga atcgttcgag ggcggtttcg acctgatcgt
     3421 ggagttcccg gacccggtcc ccgagttcga ttggatcggt cctatctggg aacgggcttc
     3481 ccggtccgcc gcccccgggt cggccttcgt gatggtcggc cgccccaccg aggccgggcg
     3541 cttcgaccgc cgcaagcctt cgggctggac ccgagccttc gagcggaaac gggacggctt
     3601 ctcgggcctg gtgtaccgcc gggagggata agcccgagtc ggcgcccgcg acaaactcct
     3661 aggagccgaa taatcgggat ttttagcgcc tatcggcgct aaaaatccac aagtccgaca
     3721 ggctcctagg ctccggagct ctccgcaggg aaggggttgg cccgatccag gacctcccgg
     3781 atcacccggt gctccggctc caaaaggccc ggatcccggt ccacgatccc ggcggcgtcc
     3841 aggcgggcgg tttccaggag gtccgcgtcc cggaccggat ccgcgaaggc cagccgcagg
     3901 gagcccgact gggcgatgcc cgcgatctcg ccgggacccc ggatgcgcag gtcttcctcc
     3961 gccagggcga acccgtccgt ggtctccttc atggccttga gacggcgctt tccctcctcc
     4021 gagaggtccc gggaatagac caggaagcag taggactggg cctcgctccg gcccacccgg
     4081 ccccggagct ggtgcagggc cgccaggccg aagcgctccg cgtgctcgac caccatgcag
     4141 gtggccgcag ccacgtccac gcccacctcg accacgctgg tggcgaccag gtaccgcagc
     4201 ccgccccggg tgaaggcctc catgacggag cgtttctcct cgtccggcaa cctcgaatgg
     4261 atgaggcctc cgggaatccc gggatagacc tcctcggcca ggtgccgggc catggcctcg
     4321 gcgtccttga gcgacccggc ctcgctgccg gagatcaggg ggtagacgaa gtaggcctgc
     4381 cgtccctccg tcagctcctt ccgaacgaac tcgtagacct tggcctcgtt gccgtgccgg
     4441 gccaggtggg tgaccacggg cttccggccc ggaggcatcg tccggatggt ggaaacctcc
     4501 aggtccccgt agaccgtcag ggccagggtc cgcggtatgg gagtggcggt catcatgagc
     4561 aggtccggtg cgccgccctt gcgggacagg gccaggcgct ggagcacccc gaagcggtgc
     4621 tgctcgtcca cgatcgccag ggccaggcgc cggtactcca cgtcctccga gaagagggcg
     4681 tgggtcccca ggacgaagtc cacctccccc gcggccaggg ccgccaggag gggacggcgg
     4741 gaggcgtccc ggaggttgcc ggtcaggaag gccaggcgca cccccagggg ctccaggagc
     4801 cgggcggcgg tctgggcgtg ctgccgggcc aggagctccg tgggggccat cagggccgcc
     4861 tgccccccgg actcgacggc cttcagggcc gccaggaagg cgaccagggt cttacccgag
     4921 cccacgtccc cctgcaagag gcgcgccatg gggcggggct tttccatgtc cgcggcgatc
     4981 tcggcggcca ccttctcctg gtccgcggta aggcggaagg ggaggcgctc caggagccgg
     5041 gggcggagcc ccgcccgggc cggggggccc gcggaccggt ccgcggggcg ctccgcccgg
     5101 gcggcggccc tgcggcgcac caggacctgg aggtagaaga gctcctcgta ggccagggca
     5161 cggagggcgt gctccgcggc ctccgggtcg cccggggcgt gcaggtcctc caaggcccgg
     5221 gccttgggcg gcaggccccg gagctcccgg atccggaccg gcacctcgtc ctccacccgg
     5281 cggccgtact cggccagggc ccgggccgcg agcttccgga cggtaccttg ggccaagccg
     5341 tccgtgaggg ggtagacggg aaggaccccc gcctcggggc cctgtccctc cgcggccgcc
     5401 tctatttcga aggaggcggc ctggagctcg ttgaaccgga gggccaggtt tccggtgacc
     5461 cgggaccggg agcccacggg gtggcggtcc gccaggaagg cccggttgaa gcagaccagg
     5521 gcggcgacgg cggtcccgtc gtccaccagg atcttgaggg tccgggtccg gccgtagccg
     5581 aaccagtcgt gggcggtcac cacccccacc gtgttcgccg ggaggccggt ccgcagggcc
     5641 tcccggaagg gcacggcccg ggagcggtcc tcgtaggccc ggggcgccct gagaaggagg
     5701 tccgccacgg tgtacacccc cagccgggcc agccgggcgc agaccgaagg acccgccccc
     5761 ttcagggcgg acacgctctg ggtcagttcc cgcaggtaca cggccgcctt ccctctctcc
     5821 ggatcaaagg tccgcggctc ggcggagcca gcggtagaac tcgagaccgg agaccggatc
     5881 cagggggcgg aagttccggc cgtccggcag ttccatgatc tcccgctcca cgcagcccag
     5941 gaccgagtcg aaccaggggg cctccagggg cacgtccggg acagggctcg cggggttcgg
     6001 ccgagcccgg taccggtcgg aatacctgcg caggagggat cggtcgttcc tccggttcgc
     6061 cgccaggtgc cagagcagga aggccgcctc cccccggcgc gcggccgcgg tacccggggc
     6121 cgcgggatcg gggtagaaac ctccggcccg aaggcgctcg aagaggatgc cgggggccca
     6181 gtccttgccg ccggtcagga agtcgaccag ggaactctcc gcctggacca gggcggccag
     6241 gtcctggagg gatacgggcc ggtcggagag cagggccgat cccgcgggcg ccgaggcgtc
     6301 ccggagggcc agggcccggc gacgctccgc cccgtagacc gcttcgtagg gctccgggag
     6361 gaagggcagg gaggcgtcga aagcggccag ggcttcggcg taccgcccct cggccaggag
     6421 gatccggccc agctcggccc ggaggcggac ctgggtctcc gcggtcttca gggcctcctc
     6481 caggagggcc ttccgcttgt cggcatttcg ctcaagccgg atctccagga cccgggcgac
     6541 ctcgtccgcg gggttcaggg agagggcctt ccggagttcc cggtccgcga cgggccggtc
     6601 ctgggccagc agggcggccc ggccggccag ggcggccaga cgcgccgacc aggtccgctc
     6661 caggacgggg cgggagccgg tctcggccag gcgggaccgg agggcatcca gggccgcccg
     6721 atccggttgg gcttccagga gcaccagatc ccgttcatgg gaatccagga cggcgtatcc
     6781 gtcctcgggc aagcgctcca cggtcaggtc cctctggacc gtcgcgcagg aggccagcag
     6841 ggccccggcg gccagggccg ccagacaggc ccgcagggcg gccgggaaag actgcggagc
     6901 gggtgccccc ggacaggtgc gggaaggccg acgggtcggg cgggccgtgg ttcgggcagg
     6961 gctcatgggg gcctcctagg gatcagggag cttggaaggt gacggcccgg acggttccgt
     7021 ccacccccgc ggaaaccagt tccgggcggc cgtccccgtt caggtccgcg aaggcgggcc
     7081 gtcccgttcc ctggacgggg aaccccgtca ggggctccag gtccgcatcg aagccgtaca
     7141 gggcgttgcc gtccccggac acgaagattt ccggccgtcc gtccccgtcc acgtcacgcg
     7201 ccgcgagcac gggttcccgg cttcccaggc cccggacgtc ggtccgggtc cggcgtccgt
     7261 ccaggcccac ccggtagagg gtccccgact cggagaccgc ccagtaggcg tccgccgcga
     7321 agatcggggt gccgtagaag actccctcca ggtcgacggg gaatcccggg acgggctcgc
     7381 cccggtcgtt ccagcaggag agagtgccgg cctgggtcag gaaggcggcc cggaaggagg
     7441 atcccagggg ggcgagcacc ggggatccgt atccgatccc ggggagggcg acgggccagc
     7501 ccgggagggc ctggccgccg gagtccagga tccagagggt cccgtcgaag ctcttggggt
     7561 agattccaag gaatccgttc cagcgggaga tcgggacgga cacgggatcc tcgaaggtga
     7621 ccggcaggga gccctcggtc ccggaggagg atacggtgag gatccccccg tcccggaggg
     7681 ggacgaggag ccggagggcc ggggaggtgc ccttggcctc gggatccggg gccagggtcg
     7741 gggccgcagc cggccgggcc ccggtggctc gcgggaagcc ctccgaggcc gcgagatccg
     7801 ggtccagcct ccagacggtg cccctgcggg agaccgccca taccgccgcc acggtgccgt
     7861 ccggcagggt ctccaggacg aagtggcccg agtcgtccag gctcagggcc ttccggatcc
     7921 ccgaggcggg gaggtaggaa accagggtgc cgccctcctc catccagtag agccggaccc
     7981 tgcggtccgg gccccggccg gcctccatgc ccccgacggg gcgccgttcc gtccggaggg
     8041 ggaagccggg gaggtcccgg gtgccccggc cgcccagggc gaccgcggag agggccagct
     8101 tcatccggcc gtccccgaag cggatcgagg ccgtgccctc gccgtagcgg cggaggaatt
     8161 ccgccagggg gcccgttccc cggagaaaga aggggacgct ccggtccagg gaatagtaga
     8221 tcatggccgc ggcgtccgcg gggatcccgg cggagacccg gcgccaggaa tccgtccggg
     8281 gcagggtctt ccccgcccgg gcttcccggg ccgcggagac cagggattcc gcggaggtcg
     8341 agacgaggag gtagccgtcc tccacgagga agtagggctg ggggacgtcc gcgcccgcgg
     8401 cgtcgaggag ggcccggatg taccagggga agacgatccg gggcacccgg atcccgtcca
     8461 ggaccgcggc ggagtcttcc cccagggtgc cgctctggaa ggcctcctgg aagaccctgc
     8521 gccttcgggc ctcgtcccgg accttcagga agaacacggg ctcggtccgg tccgaaaggc
     8581 cgaaggcccc caactcgtcc ccggaccagg acaggagcag gtcctccagg cccatgccca
     8641 gggcggcctt ggatgcggca tccgcctgcc tccaggtttc ctcggccccc aggaggggga
     8701 gggccgcgtc ccggacctgc tccaggcttc ccgcggccag gacggaaaag taccgggagg
     8761 actcgggaag gacggagagg accgccggcg gccgggagcg ggcggaaatc agggccgcca
     8821 gggcggcccg gtccgaggag accggaagct ccagggtcag ctgcacggcc gcgttcgaga
     8881 ggctcaggtc cacagcggcg aacccgggaa gcttgaggtc cgccaggaat ctgcccgccg
     8941 gatcggcatc gccccggaag ccggccagga gctccgcggt ccgcaggagg atgcggaggt
     9001 cccggggggc gcggccggcc aggagctccg cggtctcggc gtcccgtccc gctcctcccg
     9061 acgccagggc gcgctccagg agcccctcgt ccccagcgag gacaagcagg tttttccgga
     9121 ccgcggcgtg gatcaccgta tccccggccc ggtagaggaa gcgggaggtc ccggaggagc
     9181 ggctgtaggt caggcccggg atgctcagcc agggccccca gaagggggcg gtccgggtca
     9241 gggcggagcg gatccccagg tccaccgcgg ccaggtaggc cgggccgtac agggccccgt
     9301 ccatcctcac gtccgcgagg atccggaagg cccggctccg caggaaggga tcccgccgga
     9361 gggcgtccac cagggcccgg acgccggcgg cctcgggggc ggccagg
//
LOCUS       DAOTEI010000141         9406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5180_length_9406_cov_4.225430, whole genome shotgun sequence.
ACCESSION   DAOTEI010000141 DAOTEI010000000
VERSION     DAOTEI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5180_length_9406_cov_4.225430"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="P5313_12520"
     CDS             <1..248
                     /locus_tag="P5313_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81236.1"
                     /translation="EALAWNAALTVSLCFAVQGLGILRHLLERIAPTGPLRWTGILLV
                     VLLLFNLKTGAWTAGTLTVLGATETWIPYRTSKGVQT"
     gene            245..817
                     /gene="rplI"
                     /locus_tag="P5313_12525"
     CDS             245..817
                     /gene="rplI"
                     /locus_tag="P5313_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRY81237.1"
                     /translation="MKVILNNDVPNLGELGDVKEVALGYARNFLLPRGLALPYNQKVV
                     ALFEKRKTEIESLKAKKRLESSDLKTRLEAEEISLVLPAGANGKLYGAVTNATVADEL
                     LKKGFSVDRKRIEIPGRVIKSVGNYKVAIRLYEKDEATIKLVIRGQSDKEARAEAAAA
                     ATTAPKVERFKSAHVDEDEEIDERLPEDRD"
     gene            824..2161
                     /gene="dnaB"
                     /locus_tag="P5313_12530"
     CDS             824..2161
                     /gene="dnaB"
                     /locus_tag="P5313_12530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRY81238.1"
                     /translation="MAPTQLRDKVPPHNAEAEQAVLGAILLDPDSVTLVLRFLRPEDF
                     YVNANRNVFSAIISLYEKGQKPDLITLTDELRLLALLDASGGPGYVADLTSFVPSSAN
                     VEYYARIVQENSVRRALLKLSQQMTAKVFDDSVESALMLDEAQASIFDISNKRQAVSY
                     KSVKELLPDTIKMIEKLYRTKTPYTGIPSGFAELDSMTSGFQDSELVIIGARPSQGKT
                     ALALTIAAHTAIHEKIPTAFFSLEMADTALMQRMVASEARIGSEKIRTGLMKHSDFQS
                     LIDAAGRIYDAPLYIIDMPNMKLLDLRTLARRLRAERDVRIIFVDYLTLVTHENTELP
                     RWEQIASISRSLKALARELRIPVVALSQLKREAEGKQPTLADLRESGSIEQDADLILF
                     LHRDRELGRTQEERSNPIETELIVAKQRNGPTGTSRILFLPAFTRFESIAKHS"
     gene            2223..2519
                     /locus_tag="P5313_12535"
     CDS             2223..2519
                     /locus_tag="P5313_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HRY81239.1"
                     /translation="MELRQMYDFSILVNEAERMVLDELDRQLDDAESQGICTCEDCVL
                     DMAALALNSIKPLYRVSLLGTLYAQAADGTEYADHVRKAVETAVDKVRKNPSHD"
     assembly_gap    2546..2645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2850..3470)
                     /locus_tag="P5313_12540"
     CDS             complement(2850..3470)
                     /locus_tag="P5313_12540"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HRY81240.1"
                     /translation="MRTVTCPCEAVFEADLPESVDLDSRPRALEEILEGTFLSARCPE
                     CGTVVKPDLPVRVTSGSRGMDIQVVPEVDRFAFYRGAVPLPSGSEAVIGYRELVERIR
                     AVRDGVSPRALEVLKYMLLARAQQDAPEAEIVIEYAGREVDSFVFDVWGLKADEAGVV
                     RIPAAVYEKTLSELARKSSEEPFSRIFAASYRSVRILEADPDEELK"
     gene            complement(3511..4878)
                     /gene="rseP"
                     /locus_tag="P5313_12545"
     CDS             complement(3511..4878)
                     /gene="rseP"
                     /locus_tag="P5313_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRY81241.1"
                     /translation="MVVIVLGLLGLGIVVFVHELGHFLAARWMGVHVEAFSLGWGPKL
                     AGITRGGTEYRVSVFPIGGYCRMKGEEAFKAALERGDEDMPREPGSYYGASPGRRIVI
                     ALAGPVANLVFALIVAVAIAAAGYTLRTSGNKVILASEYPLVGAPVTGLPAERAGIRT
                     GDRIVAIDGVETRMYSQIQEIIFRSADKPLRLTVERDTNRIELVVVPVLDRETGAGRV
                     GLYAWIDPVVERVEPTGSAYVAGLEPGDRILSVNGQKVEHDVAFQKALLDRSRVFRME
                     IDRAGTRQVVEVVPTWDKDGHPDLGLSFRAVAFRIQADGPLDAIVKGWKDTRDTVLGT
                     LQGLAGLFRGNVKVTNAVSGPARITYMVGTVASRSLRDGWADGLSQLFNFLSLLSVGL
                     FVMNLMPIPLLDGGQILLFVVEGIRRKPLKLKTVYRYQAVGMAVVLALFLLTTVADLT
                     FFSGR"
     gene            complement(4883..6013)
                     /gene="dxr"
                     /locus_tag="P5313_12550"
     CDS             complement(4883..6013)
                     /gene="dxr"
                     /locus_tag="P5313_12550"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRY81242.1"
                     /translation="MQPKRIIILGATGSIGRSALDVAVSRPDLFRVVGLSAHSRTEEL
                     LAAGRRVPGAALCLTGADSASPEIGYGGPGAVARMLEEAQADMTLNGISGAAGLEPSL
                     QAIEAGTDLALANKETMVLAGRLVLAKAAARGVRVIPVDSEHSAVFQMVERFGAGDLE
                     EVILTASGGAFRDRPIAELPHATPEEASTHPNWNMGRKITIDSASMANKGLEVIEAIR
                     FFGLESRRVKVLIHPQSRVHALIRMRDGALYAQVSETDMRLAIQSALSWPRTETCAFG
                     RLDLAGLNLSFREPDPARFPLLGLAYRAVDAGEGYTIAYNAANETAVDLFIRRKIRFT
                     DLAGLTSAVLDLAWPSRVDSLEEVLSVDVEARAAALEAAKGF"
     gene            complement(6023..6889)
                     /locus_tag="P5313_12555"
     CDS             complement(6023..6889)
                     /locus_tag="P5313_12555"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRY81243.1"
                     /translation="MKNFLSRFLLVAVALPSLYAAAVYLPRHGHAAIIAVVLVFTAGC
                     GTEIAGLFRRKGVRAQAPLLAGLAVLPTVLLWTILRIYPGAPASALGVFVAGYGTVFL
                     AAFAPFAFPRKAEDTAESLSRSAARAFPLLYPGLLAAPIVLIADLPGDSSTAALIWFA
                     LLVFGNDSAAWAVGMLAGRHRGIVTVSPNKSLEGFLGAFAGSLAAAFLGPVLYPRALS
                     GSPWKLLVLGVLVGAAVIVGDLFESALKRSAGIKDSGTMIPGRGGFLDTFDSILFAAP
                     VFYAAIIILDLV"
     gene            complement(6886..7581)
                     /gene="uppS"
                     /locus_tag="P5313_12560"
     CDS             complement(6886..7581)
                     /gene="uppS"
                     /locus_tag="P5313_12560"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRY81244.1"
                     /translation="MVAGKAVPAHAGIIMDGNGRWAKARGLPRTEGHREGLRVAKRII
                     RAAEDLGIRYLSLYTFSTENWKRAAEEVSFLMSLITRHLRSEFDFYRDNHIRIVHSGD
                     AAGLPEDVREAIRSVMADTSGYDGITVNLAINYGGRDEIVRAARKLLRGGAVDVSEDG
                     FRAFLDAPDLPDPDLVIRTGGDMRLSNFLLWESAYAELYYSEKLWPDWSEGDLIKAVD
                     SYSCRERRYGGTP"
     gene            complement(7583..8134)
                     /gene="frr"
                     /locus_tag="P5313_12565"
     CDS             complement(7583..8134)
                     /gene="frr"
                     /locus_tag="P5313_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRY81245.1"
                     /translation="MDEVKASCEDRMRKAIAALKDEYNGLRTGRASPALLDRIRVDYY
                     GEKTPIPQVATVSVPEARLIVIQPWDRSQIAEIEKAIRTSELSLNPSNDGKVIRIAIP
                     PLTEERRKDLAKTAKNMAENCRVSIRNIRRDGIETLKKHKPDGSSEDDLKKAEEDLQK
                     LTDAYIAQANKVLEEKEKEILEV"
     gene            complement(8212..9054)
                     /gene="tsf"
                     /locus_tag="P5313_12570"
     CDS             complement(8212..9054)
                     /gene="tsf"
                     /locus_tag="P5313_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRY81246.1"
                     /translation="MEIKAQDIKTLREKTGAGMMDCKKALTEAGGDPVAAEKLIKEWG
                     LAGVEKRSGRATNEGKVFVKEEGGKVGMVEVACETDFVARNEDFIKAGAVLASLVHKK
                     EASAPDAEMEAMIRDMASVIKENIALKRVICVKAGPGEVLHTYIHGEGTLGVIVKAKT
                     DKPGDASVQAFIHDTALHIAAFRPLFLDRGRVDAAYLAEQQEIFQKQIEGDEKMKGKP
                     ANVLEGIVKGKVNKLMAEICLLDQGFVKDEKQTVAKVMADVAKAAGAALAIVDYVVYR
                     VGEN"
     gene            complement(9076..>9406)
                     /locus_tag="P5313_12575"
     CDS             complement(9076..>9406)
                     /locus_tag="P5313_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRY81247.1"
                     /translation="IVDTNCNPEGITFPIPGNDDAIRSISLFTQIIANAVVEADNETG
                     LKIIENLQEEEEAVEEVRTDARQAEEEVEIDISTYTPEEKPAAVEEKAEETAPAAVID
                     EDKLYDR"
BASE COUNT         1681 a         3107 c         2907 g         1611 t
ORIGIN      
        1 tggaagccct ggcgtggaac gccgccctga ccgtctcgct ctgcttcgcg gtccagggac
       61 tcggcatcct gcgacattta ctggagcgaa tcgctcccac cggtcccctc cgatggacgg
      121 ggatcctcct cgtcgtcctg ctcctgttca acctgaaaac gggagcctgg acggcgggga
      181 ctctcacggt tctcggcgcg accgagacct ggataccgta ccgaaccagc aaaggagtcc
      241 aaacatgaag gtcatcctga acaacgatgt gcccaacctg ggcgaattgg gagacgtcaa
      301 ggaagtcgcc ctgggatatg cccgcaactt cctgcttccc cggggtctgg cccttccgta
      361 caaccagaag gtcgtggccc tgttcgagaa gcgcaagacc gagatcgagt ccttgaaggc
      421 caagaaacgc ctggagagct cggacctcaa gactcgcctg gaagccgagg agatatccct
      481 ggtccttccc gcgggcgcca acggcaagct ctacggcgcc gtcaccaacg ccaccgtggc
      541 cgacgagctc ctcaagaagg gcttctccgt ggaccgcaag cgcatcgaga ttcccggccg
      601 ggtcatcaag agcgtgggga actacaaggt cgccatccgc ctctacgaga aggacgaggc
      661 gacgatcaag ctggtcatcc gcggccagtc cgacaaggaa gcccgtgcgg aagccgcggc
      721 ggccgccacc accgccccta aggtggagcg gttcaagtcc gcccacgtgg acgaggacga
      781 ggagatcgac gaaaggctcc ccgaagaccg ggactaagag cccatggcgc cgacgcagct
      841 cagggacaag gttcccccgc acaacgcgga agcggaacag gccgtgctcg gcgccatcct
      901 cctcgacccc gactccgtga ccttggtcct ccggttcctg agaccggagg acttctacgt
      961 caatgccaat cggaacgtct tttccgccat catttccctc tatgagaaag gccagaagcc
     1021 cgacctcata accctcacgg acgaactcag gcttcttgcc ctgctggacg cctcgggagg
     1081 gccgggctac gttgccgacc tgaccagctt cgtcccctcc agcgccaacg tggaatacta
     1141 cgcccgaatc gtgcaggaga actccgtccg cagggccctc ctaaagctct cccagcagat
     1201 gaccgccaag gtcttcgacg attccgtgga gagtgccctg atgttggacg aggcgcaggc
     1261 gtcgatcttc gacatcagca acaagcgcca ggcggtttcc tacaagagcg tcaaggagct
     1321 tcttcccgac accatcaaga tgatcgagaa gctctaccgg accaagaccc cctatacggg
     1381 catcccgagc ggcttcgcgg agctggattc catgaccagc ggcttccagg actcggaact
     1441 ggtcatcatc ggcgcccggc cctcccaggg caagaccgca ctggccctca cgatcgccgc
     1501 gcacacggcc atccacgaga agatccccac ggccttcttc tccctggaga tggcggatac
     1561 ggccctcatg cagcgcatgg tcgccagcga ggcccggata gggtccgaga agattcgcac
     1621 gggcctcatg aagcacagcg acttccagag cctgatcgac gccgcggggc gcatctacga
     1681 cgcccccctg tacatcatcg acatgcccaa catgaagctc ctggacctcc ggaccctcgc
     1741 ccggcgcctc cgggcggagc gcgacgtccg tatcatcttc gtggactacc tgaccctggt
     1801 gacccacgag aacaccgaac taccccggtg ggagcagata gccagcatct cacggtccct
     1861 caaggccctt gcccgtgaac tccggatacc cgtcgtcgca ctctcccagc tgaagaggga
     1921 ggcggagggc aagcagccca ccctggcgga tctccgggag tccggatcca tcgagcagga
     1981 cgcggacctg atcctcttcc ttcaccggga ccgtgagctg gggaggaccc aggaggagcg
     2041 ttccaacccg atcgagacgg agctcatcgt ggccaagcag cggaacggcc cgacgggcac
     2101 ctccaggatc ctcttccttc ccgccttcac ccgcttcgaa tcgattgcaa agcactcata
     2161 ggggctatac actactactg attccgacag aggcgggccg tcgagcggcc ggggagggcg
     2221 tcatggaact tcgccagatg tacgacttca gcatcctcgt gaacgaggcg gagcgcatgg
     2281 ttctggacga acttgaccgc cagctggacg acgcggagag ccagggcatc tgcacctgcg
     2341 aggactgcgt gctggacatg gccgccctgg ccctgaactc gatcaagccg ctctatcggg
     2401 tatccctgct gggcaccctg tacgcccagg ctgcggacgg cacggaatac gcggatcatg
     2461 tgcggaaggc cgtcgagacc gcggtggaca aggtccggaa gaacccgtcc cacgactgag
     2521 cgtgaagagg ccggcttcgc cggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnntcttc acggcgccga agccgtagct tcggcgattc aaccggagaa aacccggctt
     2701 cgccggcctc ttcacggcgc cgaagccgta gcttcggcga ttcaaccgga gaaaacccgg
     2761 cttgcgccgg ccctcaacgg cgccgaagcc tgaggcttcg gcgatcacca cggggattga
     2821 ttaccgctgt cgtccttcgg gaatggattc tactttagct cctcgtccgg gtccgcctcc
     2881 aggatgcgca ccgagcggta gctcgccgcg aagatccggg agaagggctc ctccgaggac
     2941 ttccgggcca gctccgacag ggtcttttcg tatacggctg cgggaatccg gaccaccccc
     3001 gcctcgtcgg ccttgagccc ccatacgtcg aacacgaacg agtcgacctc ccggcccgcg
     3061 tactcgatga cgatctccgc ctccggagcg tcctgctggg cacgggccaa gagcatgtat
     3121 ttcaacacct ccagcgcccg gggcgaaacc ccgtcccgga cggcccggat cctctccacg
     3181 agttcccggt aaccgatcac cgcctcggat cccgagggaa ggggaacggc accccggtag
     3241 aaggcgaacc ggtccacttc cggcaccacc tggatatcca ttccccggga tcccgaggtg
     3301 actcggaccg gaaggtccgg cttcaccacc gtgccgcact cggggcaacg ggcggagagg
     3361 aaggttcctt ccaggatctc ctcgagcgcc cggggacggg agtccagatc cacggactcc
     3421 ggaaggtccg cctcgaagac ggcttcgcag ggacaggtca cggttcgcat ccaaacctcc
     3481 tggttgcccc gtttctgacg ggcgcggggc ctagcggccc gaaaagaacg ttagatccgc
     3541 gacggtggtc aacaggaata gggccagcac gaccgccatg cccacggcct ggtaccggta
     3601 tacggtcttg agcttcaagg gtttgcgtcg gatcccctcc accacgaaca gtaggatctg
     3661 cccgccgtcc agaagcggga tgggcatgag gttcatgacg aacagcccga cggagaggag
     3721 ggagaggaaa ttgaagagct gggacaatcc gtccgcccat ccatccctga ggctacggct
     3781 ggccacggtg ccgaccatgt aggttatgcg cgcagggccg gaaaccgcat tggtgacctt
     3841 gacgtttcct cggaaaagcc ccgccagtcc ctggagggtc ccgaggacgg tgtccctcgt
     3901 atccttccag cccttgacga tcgcgtccaa aggcccgtcg gcctggatcc tgaacgcgac
     3961 ggcccggaag ctcaagccca ggtcgggatg tccgtccttg tcccaggtcg gaaccacctc
     4021 gacgacttga cgagtcccag cccggtcgat ttccatacgg aagaccctgg agcggtcgag
     4081 cagggccttt tggaaagcta cgtcgtgctc gaccttctgg ccgttgaccg aaagaatcct
     4141 gtccccgggc tccagccccg ccacgtacgc ggaaccggtg ggctccaccc gctccacgac
     4201 gggatcgatc cacgcataca gaccgaccct ccccgccccg gtctcccggt ccagcacggg
     4261 caccacgacc agttcgatac ggttggtgtc ccgttcgacc gtcagccgga ggggcttgtc
     4321 cgcggatcgg aagatgatct cctggatttg ggaatacatc cgggtttcga cgccgtcgat
     4381 ggccacgatc cggtccccgg tccgtattcc cgctctctcc gcaggcaacc cggtcacggg
     4441 tgccccgacc aggggatact ccgacgcgag gatgaccttg tttcccgagg tccggagggt
     4501 atacccggcc gcggctatgg ccacggccac gatcagggcg aacacgaggt tcgcgacggg
     4561 ccccgcgagg gcgataacga tacgccgccc gggagaggcc ccgtagtaac tgccgggttc
     4621 ccggggcatg tcctcgtccc cgcgctcaag ggcggctttg aaggcttcct cgcccttcat
     4681 ccggcaatag ccccctatgg ggaacaccga aacacgatac tccgtgcccc cccgtgtgat
     4741 tccggccagc ttgggccccc agcccagact gaaggcctcc acgtggacgc ccatccaccg
     4801 ggctgccagg aaatgtccca gttcatgcac gaataccacg atgcccaggc ccaggaggcc
     4861 cagaacgatg acgaccatgc tctcagaatc ctttcgcggc ttctagcgcc gccgcgcggg
     4921 cctcgacgtc cacggacagg acctcctcca ggctgtcgac ccgggatggc caagccaggt
     4981 ccaggacagc cgatgtaagg cccgccagat ccgtaaaccg tatttttcga cggatgaaga
     5041 ggtccaccgc ggtctcgttg gccgcgttgt aggctatcgt gtatccctct cccgcatcca
     5101 cggcccgata cgccaggccc agcaggggga accgggcggg atcgggttcc cggaaggaca
     5161 ggttcaggcc cgccaggtcg aggcggccga aggcgcaggt ctccgtacgc ggccaggaca
     5221 aggcggactg gatggccagc cgcatgtcgg tttccgaaac ctgggcatag agggccccgt
     5281 cccgcatgcg gatcagggca tgcacgcgac tctgggggtg gatcaggacc ttcactcggc
     5341 gagactccag accgaagaac cggatggctt cgatgacttc gagccccttg ttggccatac
     5401 tggccgagtc gatcgtgatc ttgcgtccca tgttccagtt cggatgggtc gatgcctcct
     5461 ccggggtcgc gtggggaagc tccgcgatgg gcctgtcccg gaaggctccc ccggaagcgg
     5521 tcaggatgac ctcctccagg tccccggccc cgaagcgctc gaccatctgg aagaccgcgg
     5581 agtgctcgga atccacgggg atcacccgca cgccccgggc cgccgccttg gcgagcacga
     5641 ggcggcccgc caggaccatg gtctccttgt tggccagggc caggtccgtg cccgcctcga
     5701 tggcctgcag ggacggttcc agacccgcgg cgccggaaat tccgttgagg gtcatatccg
     5761 cctgcgcttc ctcgagcatc cgggcgacgg ctccgggtcc cccgtagccg atctccgggg
     5821 aagcgctgtc ggcccccgtc aggcaaaggg cggcgccggg aacccgtcgg ccggcggcca
     5881 ggagttcctc tgtcctcgaa tgggcggaca ggccgaccac ccggaagagg tccggtcggg
     5941 atacggccac gtccagggca gagcgcccga tggaacccgt ggcgcctaga atgatgatgc
     6001 gtttcggctg cacggtgcgc ccctaaacca ggtccaggat gatgatggcg gcgtaaaata
     6061 cgggggccgc gaacagaatg ctgtcgaagg tatccaggaa tccgcccctg ccgggaatca
     6121 tggtgccgga gtcctttatg ccggcgctgc gcttcagggc ggattcgaag aggtccccga
     6181 caatgacggc ggctccgacc aggactccca gcacgaggag tttccagggg gaaccggaga
     6241 gcgcgcgggg gtacagcacg ggtcccagga aggcggccgc cagggatccg gcgaaggcgc
     6301 ccaggaagcc ctccaggctc ttgttcggcg aaaccgtcac gatgccgcga tgccgtccgg
     6361 ccagcattcc cacggcccag gcggcgctgt cgtttccgaa gaccaggagc gcgaaccaga
     6421 tgagcgccgc cgtggaggag tcgcccggca ggtcggcgat cagtacgatg ggcgccgcga
     6481 gaagccccgg atagagcagg gggaaggccc gcgcggcgga cctggaaagg gactccgcag
     6541 tatcttcagc cttcctcggg aacgcgaagg gggcgaaggc ggccagaaaa acggtcccgt
     6601 accccgcgac gaagacgccg agggccgagg ctggcgcgcc gggatatatt cggaggatcg
     6661 tccagagcag gacggtcggg agtacggcca ggccggccag gaggggagcc tgagcccgga
     6721 cgcccttgcg ccggaataat cccgctattt ccgtcccgca gcccgcggtg aacacgagca
     6781 ccaccgcgat gatggccgcg tgcccgtgtc ggggcagata caccgccgcg gcatagaggg
     6841 acggcaacgc aacggccacg agcaggaacc gggaaaggaa gttcttcatg gcgtgccgcc
     6901 gtagcggcgc tccctgcagg aataggagtc cacggccttt atcaagtccc cctcggacca
     6961 gtccggccaa agcttctcgg aatagtacag ttccgcgtag gcggattccc agaggagaaa
     7021 attggagagc cgcatgtccc cgcccgtacg gatcaccagg tccggatccg gcaggtccgg
     7081 agcgtccagg aaagcccgga agccgtcctc ggacacgtcc acggctccgc cccgcaggag
     7141 cttgcgtgct gcccgcacga tctcgtccct gccgccgtag ttgatagcca gattgaccgt
     7201 tatcccgtcg tacccggagg tgtccgccat cacggaacgg atagcctccc ggacatcctc
     7261 gggaagtccc gccgcgtcgc cggaatgcac gatgcggatg tggttgtccc ggtagaagtc
     7321 gaattccgat cgaaggtgtc gggtgatcag cgacatcagg aaggaaactt cctcggccgc
     7381 gcgcttccag ttttcggtgc tgaaggtata gagggatagg taacgtatgc cgaggtcctc
     7441 ggcggcccgg atgatccgct tggcgacccg gaggccttcc cgatgaccct cggtgcgggg
     7501 caggccccgg gccttggccc agcgcccgtt tccgtccatg atgatgcccg cgtgggcggg
     7561 aacggccttc ccggcaacca tctcagactt ccaggatttc cttttccttt tcctccagga
     7621 ccttgttcgc ctgggcgatg taggcgtcgg tcagcttctg gaggtcctcc tccgccttct
     7681 tgaggtcgtc ctcggaggat ccgtcgggct tgtgcttctt cagggtctcg atgccgtccc
     7741 ggcgtatgtt ccggatggaa acccggcaat tctccgccat gttcttggcg gtcttggcca
     7801 ggtccttgcg ccgttcctcc gtgaggggag ggatcgcgat gcggatgacc ttgccgtcgt
     7861 tggaaggatt cagggaaagc tccgaggtcc ggatggcctt ttcgatctcc gcgatctggg
     7921 accggtccca gggctggatg acgataagcc gggcttccgg caccgagacg gtggcgacct
     7981 gggggatggg cgtcttctcg ccgtagtaat ccacccggat cctgtccagc aaggcagggg
     8041 aggcgcggcc ggtacggagt ccgttgtact cgtccttgag cgccgctatg gccttgcgca
     8101 tcctatcctc gcaggaagcc ttaacctcgt ccatgtttcc tccctgacgt gccgatccat
     8161 acggagggtg tccaataagc aaccgactta ttggacaccc cggattctat ctcagttttc
     8221 gcccactcgg tagaccacgt agtccacgat ggcgagggcc gcaccggccg ccttggcgac
     8281 atccgccatg accttggcca cggtctgttt ctcgtccttg acgaagccct ggtccagaag
     8341 gcagatctcc gccatgagct tgttcacctt gcccttgacg atcccctcga ggacgttggc
     8401 gggcttgccc ttcatcttct cgtcgccctc gatctgtttc tggaagatct cctgctgctc
     8461 cgcaaggtag gcggcatcca cccgaccgcg gtccaggaac aggggccgga aggccgcgat
     8521 gtggagggcg gtgtcatgga tgaaggcctg caccgaggca tctccgggct tgtccgtctt
     8581 ggccttgacg atcacgccga gggtaccctc gccgtggatg taggtgtgca ggacctcccc
     8641 gggcccggcc ttgacgcaga tcacgcgctt cagggcgatg ttctccttga tcacgctggc
     8701 catgtcgcgg atcatggctt ccatctcggc gtccggcgcg gaggcttcct tcttgtggac
     8761 cagggacgcc aggacggcgc cggccttgat gaagtcctcg ttgcgcgcca cgaagtccgt
     8821 ctcgcaggcc acttcgacca taccgacctt gccgccctct tccttgacga acaccttgcc
     8881 ctcgttggtg gcgcggccgg agcgtttttc gactcccgca agcccccact ccttgatgag
     8941 cttctcggcg gcgacgggat ccccgcccgc ctccgtgagg gccttcttgc aatccatcat
     9001 accggctccg gtcttctccc ggagggtctt tatgtcctga gccttgatct ccatttcgtt
     9061 ctccttgacg gcttactagc ggtcgtacag tttgtcttcg tcgatgacgg cggccggagc
     9121 cgtttcctcg gccttctcct cgacggcggc gggcttctcc tcgggagtgt aggtggagat
     9181 gtcgatctcg acctcttcct ccgcctggcg tgcgtccgtc cggacttcct cgaccgcttc
     9241 ctcctcttcc tggaggttct cgatgatctt gaggcccgtc tcgttgtccg cctcaaccac
     9301 cgcgttggcg atgatctggg tgaaaaggga gatggaccgg atggcgtcgt cgttgcccgg
     9361 gatggggaag gtgatgccct cgggattgca gttggtgtcg acgatg
//
LOCUS       DAOTEI010000142         9371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5201_length_9371_cov_5.116144, whole genome shotgun sequence.
ACCESSION   DAOTEI010000142 DAOTEI010000000
VERSION     DAOTEI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5201_length_9371_cov_5.116144"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="P5313_12580"
     CDS             <1..1079
                     /locus_tag="P5313_12580"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRY81248.1"
                     /translation="LATDLRDLRERVGRVVLGLDKSGKQVTTEDLGVAGAMTILLKDA
                     LAPNILQTLEEQLAFVHAGPFANIAHGNSSILADLVGVKVGDYLVTESGFGADMGFEK
                     FVDIKCRASGIYPDAAVLVATVRALKMHGGGPKVVPGKPLAPEYKEENRELLKKGLVN
                     LKAHLGILRKFGVPAVVAINAFPTDTPAEWDLIRQAALDAGASDAVTAFHWAEGGEGA
                     APLAEAVEKAASKPSDVKPLYSLDLSLERKIEKIATEVYGAGSIEYSPAARKALEGYS
                     KLGYDKLPVCMAKTQYSLSHDPALKGAPSGFEFPVSDIRLSAGAGFIYPIVGEINTMP
                     GLPSKPGYIGMDIDPDTGRITGLS"
     gene            1255..2286
                     /gene="lgt"
                     /locus_tag="P5313_12585"
     CDS             1255..2286
                     /gene="lgt"
                     /locus_tag="P5313_12585"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRY81249.1"
                     /translation="MTPLYTLYPSWISPEIIPGLPFRWYGMMYLVAFGITYLLFQRQV
                     RREKLGWSEDEVSNFFLAAILGLILGARLFGTFLYEPTGLYWRKPWLVIWPFDEAGRF
                     VGFQGMSFHGGLVGVVIGTVIYARRRALDYWEWADRMAVSVPLGYTFGRLGNFINGEL
                     WGKVTSGPLGMVFPHAERFPTSEAWVREVAGKAGMALDSARINLPRHPSQLYEAFFEG
                     IVLWLVLWFLVRPRRPYKGFAVGTYLIGYGAIRFAIEYLREPDSGLGYIIKLGDPDAP
                     IHTFTTLWNFSMGQILCFLMIAGGAGFLAWRKSASAREAAAAQSARSGAGDPGRRKKE
                     ARKLRKKLG"
     gene            complement(2434..3036)
                     /locus_tag="P5313_12590"
     CDS             complement(2434..3036)
                     /locus_tag="P5313_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81250.1"
                     /translation="MSAKNRENGRETRPDSPAPDRLVPFLTAPSLPVRTARVAGTGGL
                     VFLVAWYVSYLFLPEGLLKSGPTGRFAAGGALVAAAILAGNLLVRLRSLPAGYLAAAW
                     GFLYAGVLAGTNSFGVPYSVRITPTFRVFSRGIIYEMAAWAILAAASCGWAFFELDPP
                     GSGFRRTESRALRAELAAAAAGILILAAALALEAAGILPA"
     gene            complement(3017..3373)
                     /locus_tag="P5313_12595"
     CDS             complement(3017..3373)
                     /locus_tag="P5313_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81251.1"
                     /translation="MPGLTDWAAGLMDQARDTYGVNPVVFLSVFLGSGPVFYYSLFRL
                     LRSLARRDGRGTGLWSAVFLAATAAPYLYVLLFGRNLPVWVYGILVLLIGQGVWSLAR
                     RLRKNKKGDPNEREEP"
     gene            complement(3423..4526)
                     /locus_tag="P5313_12600"
     CDS             complement(3423..4526)
                     /locus_tag="P5313_12600"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRY81252.1"
                     /translation="MPRSAAKTFGILTSGGDCPGLNAAIRGVAKAACDAYGMRAVGIS
                     NGYRGLIQGDSRDLKSADFSGILTLGGTILGSSREKPFKGRDDLAETDADAGMSAPEL
                     IRENYKNMGLDALVVLGGNGTQTTAHLLQESGLNVIGLPKTIDNDLWGTDVTFGFHSA
                     VDIATEAIDRLHSTALSHNRVMVIELMGHKAGWLALFAGIAGGGDVVLLPEIPYEIGS
                     IARHLTNRAKEGKSFSIVAVAEGALSAEEAKLSKKDRKKAREKMPFPSIGYRVASEIQ
                     EAVGMETRVTVLGYLQRGGVPSPYDRLLATAFGTEAAAMLNRGDFGKMVCLSGERIGA
                     VPLSEVAGKIKPVPRDHRLIRTARLVGTCMGDD"
     gene            complement(4540..5250)
                     /locus_tag="P5313_12605"
     CDS             complement(4540..5250)
                     /locus_tag="P5313_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81253.1"
                     /translation="MKKTTLVAPYDEPLARAVAVEARVLGRSVAALLPGYGEPREARK
                     GRRGEPAEAVDPDADLHGPAPGETPIPWNPPSFVSARSAVLDASVRFGSVDEAVLVVS
                     PGPAPDLTAKPAELERVLQDRVFSVLWLARELLTHFSARGSAVGPGRLVLVLADRGLP
                     PRDPVGAAAFGAVSAFAAALAESAPDAPYDVWAVQDSCPQDDLAAQYIRRILEAPPER
                     RPGRALRFTGKSTLFNRP"
     gene            5301..6968
                     /locus_tag="P5313_12610"
     CDS             5301..6968
                     /locus_tag="P5313_12610"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRY81254.1"
                     /translation="MIVALAQIDAVVGDFAGNRARIARLAREALEREPRVSLVVFPET
                     ALCGYPPMDLLDQESFAEGSLAALRALQKDLPGGPAVAVGYVDRNRSGSGKPLVNAVA
                     VIRDGRIVFTQEKTLLPTYDVFDEARYFEPARERRILELGGLRIGFAICEDIWWEEPA
                     VPGSRYPVDPVRDLLDLGPDLVVVPSASPFHAGKQETRLRLASNLAREASAPVLYCNL
                     VGATDSLVFDGRSFALDPRGNLVLQAGWEEGLTFVDTGAVGTSARDPADRWDETERAL
                     VSGIRGYMGKCGFKKALVGISGGIDSAIVAVLAAQAVGAENLTLLGMPSRFSSEGSIS
                     DSRYLAQALGARMEVLPIEGPFSAYLDLFELHFRGLPFGLAEENLQARIRGALLMTWS
                     NKFGSLLLTTGNKSELACGYCTLYGDMCGALAPIGDLLKTEVYALSRAVNERRRSEGR
                     TPPIPEATLTKPPSAELRPGQTDQDSLPPYEVLDAILELYIVRNLSGPEIVDRGHDPA
                     LVRRVLQMVGRAEYKRRQAAPVLKVSPRAFGMGRRIPIARVLHETDA"
     gene            6965..8179
                     /locus_tag="P5313_12615"
     CDS             6965..8179
                     /locus_tag="P5313_12615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRY81255.1"
                     /translation="MSRGATHGNEALRASSRLPRRFPAIRAFARAGRAAAASIVLAGL
                     VPTLLLAACQPRFPGAGSPETSAPGETVSVPLSLEGARSPGPVTLLACGDVLLSRTVA
                     DMIDRNGYRWPFRHVRDLVSSADIAFCNLENPASFTGSPYPGKPAEVTFRAPPGALFG
                     LKWAGFDVVSLANNHMNDYGPAAIRETLDYLDLLGIARTGAGIDEASARAPAVLEARG
                     ARVGFLAYAEGIWSVLPAASGRPGVALADPARLAADIGSLRKTARPDYIVVSVHWGEE
                     HERHPRQAQRDFGRAAIDAGADLVLGHHPHVLQSLERYREGLIVYSLGNFVFDMAADP
                     TYDTAALRITLAEGRILAAEVLPLRIERRDYAPRPAAPEEAGRILAGLRDTSRLLGTT
                     VEIGEGRGRVIF"
     gene            8266..>9371
                     /locus_tag="P5313_12620"
     CDS             8266..>9371
                     /locus_tag="P5313_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81256.1"
                     /translation="MIRSRRYPRPALPAAAALLAVLFAAACDDGIGLLTMDERQALYR
                     AEASSKGSALPVSAANPGAILDPSEGIRVSFEALRGSEEPAELVVTLRAGSGTPSVAV
                     RYRTGQAPAAASGESEVREVRTPSLASFSAVLEVPAEFPDGYYELVLDARSARSESLS
                     LSEIGLFLSRTPLPSPSILAYPANPAPGSRILLVANLAGYEGRDPWLRWTVDGTVRAE
                     GPASAGTDKLLWPAPQAGSPAAVGLSLYPSAPPAGSAFTFPPPRSASASLLVAPGTPE
                     TADEFSRAGRFRALFRMEDTPNYAGAPGGTAEFLQSPRLDVHPGGFGLVLGGESGAGI
                     RARALLLPARDGVLQSCTILARLVPSRSGGGILFR"
BASE COUNT         1374 a         3344 c         3169 g         1484 t
ORIGIN      
        1 ccctggccac ggaccttcgg gatctccggg agcgggtcgg acgcgtcgtc ctgggcctgg
       61 acaagtccgg caagcaggtg accaccgagg acctgggggt ggcgggggcc atgaccatcc
      121 tcctgaagga cgccctggcc ccgaacatcc tgcagaccct ggaggagcag ctggccttcg
      181 tccacgcggg tcccttcgcg aacatcgccc acggcaactc ctccatcctg gcggacctgg
      241 tgggcgtcaa ggtcggggat tacctggtca cggaaagcgg cttcggggcg gacatgggct
      301 tcgagaaatt cgtggacatc aagtgccggg ccagcgggat ctacccggac gccgcggtcc
      361 tggtagccac cgtccgggcc ctcaagatgc acgggggcgg acccaaggtc gttcccggca
      421 agcccctggc cccggaatac aaggaggaga accgcgaact cctcaagaag ggcctcgtga
      481 acctgaaggc ccacctgggc atcctgcgga agttcggggt ccccgcggtg gtcgccatca
      541 acgcattccc cacggatacc ccggcggagt gggacctgat ccgccaggcg gccctggacg
      601 cgggggcctc ggacgcggtc acggccttcc actgggccga gggcggcgag ggcgcggctc
      661 cccttgcgga ggcggtggag aaggcggcct ccaagccgag cgacgtgaag cccctgtact
      721 ccctagacct ctccctggag cggaagatcg agaagatcgc tacggaggtg tacggagccg
      781 gctcgatcga gtacagtccc gccgcccgga aggccctgga gggatactcc aagctcggat
      841 acgacaagct tcccgtgtgc atggccaaga cccagtattc cctgagccac gatccggcgc
      901 tcaagggagc gccgtcgggc ttcgaattcc cggtcagcga catccggctc tccgcgggcg
      961 cgggcttcat ctaccccatc gtcggggaga tcaacaccat gccgggcctg ccctccaagc
     1021 cgggctacat cggcatggac atcgatcctg atacggggag gatcacgggg ttgtcttgag
     1081 agtaggtgaa taggtgaagc gtgaataggt gaataggggt tgatgccagg ttcctaaaac
     1141 atcctgcgct atcgcgtggc ctgtttccgc gccctctcta tttacttatt cacccattca
     1201 cctattcact tgccccacat tgaactttaa gttgtcatcc cgtagactgt ccccatgact
     1261 cctctgtaca ccctctatcc ttcgtggatt tccccggaga tcatcccggg gctgcccttc
     1321 cggtggtacg ggatgatgta cctggtggcc ttcgggatca cctacctgct cttccagcgc
     1381 caggtgcggc gggaaaagct gggctggtcc gaggacgagg tctccaactt cttcctggcc
     1441 gccatcctgg ggctcatcct gggagcccgg ctcttcggaa ccttcctgta cgagcctacg
     1501 ggcctgtatt ggcgcaaacc ctggctggtg atctggccct tcgacgaggc cggccgcttc
     1561 gtgggcttcc aggggatgag cttccacggg gggctcgtcg gagtcgtgat cgggaccgtg
     1621 atctacgccc gcagacgggc cttggattac tgggagtggg cggaccggat ggcggtgtcc
     1681 gtccccctgg gctacacctt cggccgcctg ggcaacttca tcaacggcga actctgggga
     1741 aaggtgactt cgggccccct gggcatggtc ttcccccacg cggagcgttt cccgacctcg
     1801 gaagcctggg tccgggaggt cgccgggaag gcggggatgg ccctggactc cgcccggatc
     1861 aacctgcccc ggcacccctc ccagctttac gaggccttct tcgaggggat cgtgctctgg
     1921 ctggtgctgt ggttcctggt acggcctcgg cgtccctaca agggcttcgc ggtgggcacc
     1981 tacctcatcg gatacggggc gatccgcttc gccatcgagt acctgaggga gcccgattcc
     2041 ggcctgggct acatcatcaa gctgggggac ccggacgccc cgatccatac cttcacgacc
     2101 ctgtggaact tctccatggg ccagatcctc tgcttcctga tgatcgccgg gggggccgga
     2161 ttcctggcct ggcggaagag cgcgtcggcg cgggaggcgg ccgcggcgca gtcggcccgg
     2221 agcggcgccg gggaccccgg acgcaggaag aaggaagccc ggaagctccg caagaagctg
     2281 ggctaggagc ctgtcggact tgtggatttt tagcgccgat aggcgctaaa aatcccgatt
     2341 atccggctcc tttggagttt gaaaagtaga aaaatcgcat gaatgcgatt tttctattat
     2401 taaggtgtgc aagcccgaca aactcctagc cgatcaggcc ggcaggattc ccgcggcctc
     2461 caaggccagg gcggcggcca ggatcaggat ccccgcggcg gccgcggcca gctcggcccg
     2521 gagcgcccgg gattccgtcc gcctgaaccc ggatcctgga gggtccagct cgaagaaggc
     2581 ccagccgcag gatgcggcgg ccaggatcgc ccaggccgcc atctcgtaga tgatcccccg
     2641 ggagaagacc cggaaggtcg gggtgatccg cacggaatag ggcaccccga aggagtttgt
     2701 gcccgccagg accccggcgt acaggaagcc ccaggccgcc gccaggtagc ccgcgggcag
     2761 gctccgtagc cggaccagga ggttcccggc caggatcgcc gccgcgacga gcgccccgcc
     2821 cgcggcgaac cttccggtcg gcccggattt cagcaggccc tccggcagga agaggtagct
     2881 cacgtaccag gcgaccagga agaccaggcc gcccgtaccc gcgacgcggg cggtccggac
     2941 cggcagggac ggggccgtca ggaagggtac cagcctgtcc ggggcagggg agtccggtct
     3001 cgtctcccgg ccattctcac ggttcttcgc gctcattcgg gtcccccttc ttgtttttcc
     3061 tcaaccttcg tgccagggac cacacgccct gtccgatcaa cagcaccagg attccgtaca
     3121 cccagaccgg gaggttccgg ccgaaaagca ggacatacag gtagggagcc gccgtggcgg
     3181 ccaggaacac cgcgctccac agccccgtcc cgcggccgtc cctgcgggcc agggaccgca
     3241 ggagccggaa gagggaatag tagaagaccg ggccggatcc gaggaagacg gacaggaaca
     3301 cgacgggatt caccccgtag gtgtcccggg cctggtccat caggcccgcc gcccagtccg
     3361 tcagtcccgg cacggttcct ccccggaggc cgccgtggac cggcccccga gaaccggtcg
     3421 cctcaatcgt cccccatgca ggtgcccacc aggcgggcgg tccgtatgag ccggtggtcc
     3481 cggggcacgg gcttgatctt gccggccacc tccgaaaggg gcaccgcgcc gatgcgctcc
     3541 cccgagaggc agaccatctt gccgaaatcc ccccggttca gcatcgccgc ggcctcggtg
     3601 ccgaaggctg tggccaggag ccggtcgtag ggactgggga cgccgcccct ctggaggtag
     3661 cccagcacgg tgacccgagt ctccataccg acggcctcct ggatctccga agccacccgg
     3721 tagcctatgg aagggaacgg catcttttcc cgggccttct tccggtcctt cttggacagc
     3781 ttggcctcct ccgcggaaag ggctccctcc gcgaccgcca cgatggagaa gctcttgccc
     3841 tccttggccc ggttggtgag gtgccgggcg atggagccga tctcgtaggg gatctccggc
     3901 aggaggacca cgtccccgcc cccggcgatc cccgcgaaga gggccagcca cccggccttg
     3961 tggcccatga gctcgatgac catcacccgg ttgtgggaga gggccgtgga gtggaggcgg
     4021 tcgatcgcct cggtggctat gtccacggcg gagtggaagc cgaaggtcac gtccgtgccc
     4081 cacaggtcgt tgtcgatggt cttgggaagt cctatgacgt tgagtccgga ttcctggagg
     4141 agatgggccg tggtctgggt cccgtttccg cccaggacga ccagggcgtc cagtcccata
     4201 ttcttgtagt tttcccggat gagctccggg gcggacatcc ccgcgtccgc gtcggtctcc
     4261 gccaggtcgt cccgtccctt gaagggtttt tcccgagagg aacccaggat cgtgcctcct
     4321 agggtcagga tccccgaaaa atccgcggac tttaggtccc gggaatctcc ctggatcagg
     4381 ccccggtagc cgttggagat ccccacggcc cgcatgccgt aggcgtcgca ggcggccttg
     4441 gccacccccc ggatcgcggc gttcagcccg gggcagtccc ccccgctggt caggatgccg
     4501 aaggtcttgg cggcgctccg gggcatggtt cctcctgggt cagggccggt tgaagagggt
     4561 gctcttgccc gtgaaccgga gggcccggcc cggccggcgc tccggggggg cttcgaggat
     4621 gcggcggatg tactgggccg ccaggtcgtc ctgggggcag gagtcctgga ccgcccagac
     4681 gtcgtagggg gcgtccgggg cggattccgc cagggccgcg gcgaaggccg agacggcccc
     4741 gaaggccgcc gcgccgacgg gatcccgggg gggtagaccc cggtccgcca ggaccaggac
     4801 cagccggccg ggtccgaccg cggagccccg ggccgagaaa tgcgtcagga gttcccgggc
     4861 gagccagagg acggaaaaca cgcggtcctg gaggacccgt tccagttcgg cgggcttggc
     4921 ggtcaggtcc ggtgcggggc ccggggaaac caccagcacc gcctcgtcca cggacccgaa
     4981 gcgcacggag gcgtccagga cggcggagcg ggcggatacg aaggaggggg gattccaggg
     5041 gatgggggtt tcccccgggg cgggaccgtg aaggtccgca tccgggtcta cggcctccgc
     5101 gggttccccg cgacggccct tccgcgcttc ccggggttcg ccgtacccgg gcaggagggc
     5161 ggcgacggaa cggcccagga cgcgggcttc cacggccacg gcccgggcca ggggctcgtc
     5221 gtagggagcc acgagcgttg tctttttcac ggccccagtg tactatggcg accgtcgctc
     5281 cgcaaggcga ggaggctccc gtgatcgtcg ccctggctca gatagacgcg gtcgtcggag
     5341 acttcgccgg aaaccgcgcc cgtatcgccc ggctggcccg ggaagccctg gagagggagc
     5401 cccgggtctc cctcgtcgtc ttccccgaaa cggccctctg cgggtacccc cccatggacc
     5461 tcctggacca ggaatccttc gcggagggca gcctggccgc cctccgggcc ctgcagaagg
     5521 acctgcccgg cggcccggcc gtggccgtcg gttacgtgga ccgcaaccgc tcgggttccg
     5581 gcaagcccct ggtaaacgcg gtcgcggtga tccgggacgg ccggatcgtc ttcacccagg
     5641 aaaagaccct ccttcccacc tacgacgtct tcgacgaggc ccgctacttc gagcccgccc
     5701 gggagcgtcg gatcctcgaa ctgggcggtc tgcggatcgg attcgccatc tgcgaggaca
     5761 tctggtggga ggagcccgcg gtgcccggca gccgataccc ggtggatccc gtccgggacc
     5821 tcctggacct cggcccggac ctggtcgtgg tgccctccgc ctcgcccttc cacgcgggca
     5881 agcaggagac ccgcctccgc ctggcctcca acctggcccg ggaggcttcc gccccggtcc
     5941 tctactgcaa cctggtcggc gccacggact ccctggtctt cgacggccgc tccttcgccc
     6001 tggatccccg ggggaacctc gtcctccagg cgggctggga ggaggggctg accttcgtgg
     6061 acaccggggc cgtcgggacg tccgcccggg accccgcgga ccggtgggac gagaccgagc
     6121 gggccctggt ctccggcatc cggggctaca tgggcaagtg cggctttaaa aaggccctgg
     6181 tcgggatctc cgggggcatc gattccgcca tcgtggcggt gctggccgcc caggcggtcg
     6241 gggcggagaa cctgaccctc ctgggcatgc cctcccggtt ctcctcggag gggtcgatct
     6301 ccgattcccg gtacctggcc caggccttgg gggcacggat ggaggtcctg cccatcgagg
     6361 ggcccttctc cgcctaccta gacctgttcg agctccactt ccggggcctt cccttcggcc
     6421 tggcggagga gaacctccag gcccgcatcc ggggagccct gctcatgacc tggtccaaca
     6481 agttcggttc cctcctcttg accacgggca acaagagcga gctcgcctgc ggctactgca
     6541 ccctctacgg ggacatgtgc ggggccctgg cgcccatcgg ggacctcctg aagaccgagg
     6601 tctacgccct ctcccgggcg gtgaacgagc gccggcgctc ggaaggccgt acccccccga
     6661 tcccggaggc caccctgacc aagcccccca gcgcggagct ccgccccggc cagacggacc
     6721 aggactccct gcccccctac gaggtcctgg atgccatcct ggagctctac atcgtccgga
     6781 acctctcggg acccgagatc gtggaccggg gccacgatcc cgccctggta cggcgggtcc
     6841 tccagatggt cggccgggcg gagtacaagc gccgccaggc cgccccggtc ctcaaggtgt
     6901 ccccccgggc cttcggcatg ggccgccgca ttcccatagc ccgggtcctg cacgagacgg
     6961 acgcgtgagt cgcggagcga ctcacggcaa cgaagccctg cgggcttcgt cgaggttgcc
     7021 gcgacggttt cccgcgatcc gagcctttgc ccgggcggga cgggccgccg ccgcgtcgat
     7081 cgtccttgcg ggcctcgttc cgacccttct ccttgcggcc tgccagcccc ggttccccgg
     7141 cgccggatct cccgagacct ccgccccggg ggagacggtc tccgtacccc tctccctgga
     7201 aggcgcgaga tccccgggtc ccgtcaccct cctggcctgc ggggacgtgc tcctgtcccg
     7261 cacggtcgcg gacatgatcg accggaacgg gtaccgctgg cccttccgcc atgtccggga
     7321 cctcgtgtcc tccgcggaca tcgccttctg caacctggag aacccggcct ccttcacggg
     7381 ctccccctat ccggggaagc cagcggaggt caccttccgg gctcctcccg gcgcgctgtt
     7441 cgggctcaaa tgggcgggct tcgacgtggt ctccctggcc aacaaccaca tgaacgacta
     7501 cggccccgcg gcgatccggg agaccctgga ctacctggac ctgctgggaa tcgcccggac
     7561 cggggccggg atcgacgagg cctccgcccg ggcccccgcg gtcctggagg cccggggcgc
     7621 ccgggtgggc ttcctggcct acgcggaggg catctggagc gtcctgcccg ccgcctccgg
     7681 gcgccccggg gtggccctcg cggaccccgc ccggttggcc gcggacatcg gatccctgcg
     7741 gaagaccgcg cggccggact acatcgtcgt gtccgtccat tggggcgagg agcacgagcg
     7801 gcatccccgg caggcccagc gggacttcgg ccgtgcggcg atcgacgccg gggcggacct
     7861 ggtcctgggg caccaccccc acgtcctgca gtccctggaa cggtaccggg agggcctcat
     7921 cgtctactcc ctgggcaact tcgtcttcga catggccgcg gatcccacct acgataccgc
     7981 ggccctccgg atcaccctgg cggaaggccg gatcctggcc gccgaggtgc ttcccctgcg
     8041 gatcgagcgc cgggactacg ccccccgccc cgccgccccc gaggaggcgg gccgcatcct
     8101 ggcggggctc cgggacacct cgcgactcct gggcacgacc gtggagatcg gggagggccg
     8161 gggacgcgtg atcttctagc gggctggcgc cgggggagta aaatcgcgat acttgttcct
     8221 gagatgcccg ggactccgtg atatggtatt gaggaaaggc cccagatgat ccgatcccgt
     8281 cgataccccc ggccggcctt gccggcagcg gccgcactcc tggcggtcct cttcgcggcc
     8341 gcctgcgacg acgggatagg tctgctcacg atggacgagc gccaggccct gtaccgcgcc
     8401 gaggcctcct ccaagggctc ggccctgccg gtcagcgcgg cgaatcccgg cgcgatcctg
     8461 gacccctccg agggcatccg cgtctccttc gaggccctgc ggggctccga ggagcccgcc
     8521 gaactggtcg tgaccctccg ggccggctcg ggcaccccgt ccgtcgcggt tcggtaccgg
     8581 acgggccagg ccccggccgc ggcttccgga gaatccgagg tccgggaagt ccggaccccg
     8641 tccctggcct ccttttcggc tgtcctcgag gttcccgcgg aattccccga cggctattac
     8701 gagctggtcc tggacgcccg gtccgcccga tccgaatccc tctccctgtc cgagataggc
     8761 ctcttcctgt ccaggacccc cctgccgagc ccttccatcc tggcctaccc cgccaacccc
     8821 gccccgggat cccggatcct cctggtggcc aacctggcgg gctacgaagg ccgggacccc
     8881 tggctgcggt ggaccgtgga cgggacggtg cgcgccgagg gccccgcctc cgccggaacg
     8941 gacaagcttc tgtggcccgc tccccaggcg ggttctcccg cggccgtcgg gctgtccctc
     9001 tatccctcgg ctcccccggc gggctcggcc ttcacctttc cacccccccg gagcgcctcc
     9061 gccagcctcc tggtggcacc gggtaccccc gagaccgcgg acgaattctc ccgggccggc
     9121 cgattccggg ccctcttccg catggaggac accccgaact acgcgggtgc gccgggaggc
     9181 accgcggagt tcctgcagag cccgcggctg gacgtccatc cgggcggctt cggcctggtc
     9241 ctgggcgggg agtccggggc cggcatccgc gcccgcgccc tgctcctgcc ggcccgggac
     9301 ggagtgctcc agtcctgcac gatcctggcc cggctggttc cctcccggtc cggggggggc
     9361 atcctgttcc g
//
LOCUS       DAOTEI010000143         9352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5214_length_9352_cov_5.832311, whole genome shotgun sequence.
ACCESSION   DAOTEI010000143 DAOTEI010000000
VERSION     DAOTEI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5214_length_9352_cov_5.832311"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="P5313_12625"
     CDS             complement(<1..1571)
                     /locus_tag="P5313_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81257.1"
                     /translation="MTERRRRKPGIPARVLAVFLAASFAAAASAQAPDLQALEAEAAA
                     AAEAGDYGGAAAILRRARSLFPGDPRPAVLLGDLYTDRSLHALALDEYLAAESLDPAD
                     TGTLQRIADSYGYLNREEDSIRYLRRLLDLDPGSTRAVGDLGWMLFKTHRLNEGIALL
                     EDAERRLGPDIGFSMTLGTLRAELFEYEESRRRYEEAIAGARAEGYSRFTAVALYNLS
                     LLESRFRLWARALEAADRSLLAESRSSGYLARGELRLRRMELDAAQADFASAFGVDTT
                     PLARLGLAESALAAGRLDEALAWVREVADLEDHPWMASFGTDPDSFAMDLHELLSEIH
                     RGRARVEKARPKRDLRDGAASWASRAVESLRAAWHEGLFRKYARAVAEAYAREGAELP
                     AAVHAYLAFRGYPRRAARYLGRARALEEAWVPEARPSNDLEAALLARNPRAVEAALAT
                     LDPAWERDLTEEGLGGLAEILARRGRKAELRSVLERLWILNPAGVGRRGSGSRGARGR
                     TGARVPGGEQVIGSRG"
     gene            complement(1568..3733)
                     /locus_tag="P5313_12630"
     CDS             complement(1568..3733)
                     /locus_tag="P5313_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRY81258.1"
                     /translation="MSGSSRVLSVPARIFRTLALLLLTYQARLLLADLVSLPRALGLC
                     LLLSAAGVLLGRTRLRPWVRLAAAGVLPFALRLAFFAAFGIAGAILPGLGTDGLFPAF
                     DETFRPLLPLWIWSVVASDAAERNRSFAVFEAGLNLALLALLFASQGGFRVTLYSHPS
                     LLAAVAAALLFLSLAVLALLAPRGSGRSPGFPFRLAVLFAALALVLLALLGRYSEGAA
                     SRGGGLLKPTLFRFDLARYLKLETEISLSDDLVLIARKDPVDPNVLVRRFVLSEYDRR
                     GGFRPGPGDEGIPEILPREPGSWEVPPWEGRQSLEQEYWILNLEPGALLGVDAPVSVV
                     PWKLEPGSSFASAYALSSRAAVPALSLLLGDFDPARTPPAFALSESDRDRYTRWGDDP
                     RIRALAEEVTAEAATPYDRVTAVLSYLRENYRYSLKPGAAPDGNQLSHFLFRSRKGYC
                     SYFAFSMTVMLRSLGIPARVAAGFFLDPDTAVLEFHPVRADMAHAWTEVWFGEYGWIA
                     FDPTSREPAPGESFEESRGTDPALLEGLLREILRGAREAATPEGPGPASDRRPAWDLR
                     RAAAWVLRRSWLLAPGLWLALAVLGRGIRALRLGLLKDRRGRLLAAWESLEDALSSAG
                     KHRSRGESIVDFAGRLDREGRGTSAEDLEGLARDWERAVHDPAPNSEEVRDLEARIRG
                     FRGRVRSGIPAPRRVLAFLDLRRGLRPLPGGPGRRGGRR"
     gene            complement(3717..5054)
                     /locus_tag="P5313_12635"
     CDS             complement(3717..5054)
                     /locus_tag="P5313_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY81259.1"
                     /translation="MRNPEARGRRRSVPGAGPRLTRRGLGFSLLSTGLLAAGILRSEL
                     GALISGAGLAAAVLASLAGVLLEGRRRDRALGADPEALRPSLSPPSRDGDPDLRALFR
                     IENRGVPLPRAPGISAWVELRCGSRQGRHAEAAAALPAPGSSGAAGFPPSGPLARGVY
                     RGRAAAVAGDAFGFWHSRRELPGILETAALPEPSAAAAAVPRGSGGQRADVSDRRIRG
                     EDRFDSRPYLPGDDPRRLHWKLYARFGDLFVRPGDLSPPPRKTVRILLDTERPAWLAG
                     EAGDYYLDRIVSAALGYARYLEDRGLEVRLSAPGSPDAPPDPPGRLYWLADLAWDADG
                     AGAVNPGPDPLAVFGAPGAVRRGAVLAERKAAGFDTVLIIPDLPETGPAGFWARFFLV
                     PDGRPAKSGASGVPRAPGHGPETGRALRAAFRTALDRETRVLSGAGGFDVRVL"
     gene            complement(5062..6051)
                     /locus_tag="P5313_12640"
     CDS             complement(5062..6051)
                     /locus_tag="P5313_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY81260.1"
                     /translation="MNQIGGETPSVLDVRASAEILADLAAGMGTVLRGKDGFVRDFLA
                     CAAAGGHLLVEDLPGLGKTTAAKTFAALLSRDPDSPALFRRLQCTPDLLPYDVTGVDV
                     FDPDARAFVFHPGPVFANVLLADELNRTPPKVQSALLEAMAEGQVTTAEATRPLPEFF
                     FVAATQNPVETEGTYPLPAAQLDRFLMRVSLGYPDRDAEISILRDDPSVNVLTGLSPR
                     RSVEELLFVRRTARAVWADPGLMGSLADLVSVTRSHPGILLGASPRAALGLLAAARAR
                     ALQEGRGFVVPQDIVDLAAPVLAHRLRTRDPGFDAARWLREEAAARTDPLRRT"
     gene            complement(6133..6933)
                     /locus_tag="P5313_12645"
     CDS             complement(6133..6933)
                     /locus_tag="P5313_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81261.1"
                     /translation="MRTGTPSPAAIAALAFFAAVLPLAADPLAARVPADRAAVLIRGE
                     ELRRSSTGREAAVAWSPSHPLAAGVQSDLRAEGPDVVVEALYLWKKPRSAAPATELLT
                     VYNVLRAVGTLQGIEYWSASRNTMRLFYEESWRVVSLEDRTRVPDQRVSVVPPAETFT
                     VWQKDLSFGGNLNRLDYRTAPDGVLLESVNLTRMYYKFVPMAAPGALKVRVLVLNTDE
                     GILFWAVSSARAAVVPGVRGKLEDSFGNRAEAVFRWFSRNVEEAWKTL"
     gene            7110..8774
                     /locus_tag="P5313_12650"
     CDS             7110..8774
                     /locus_tag="P5313_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRY81262.1"
                     /translation="MTVVTLTLEMIGALGLFLYGMKVLSDGIQRAAGDRLKGALNQMT
                     RSPLAAVLTGLGVTSLVQSSSATTVMVVSFVNAGLLSLIQAVGVIMGANIGTTVTAWI
                     VSLVGFKFKITLLAVPLVGVGFILTTYLKTRNKSLRSYGDALIGFGILFIGLDFLSHT
                     LPKPSPEVLHFLARFSGLGLVSVLISVLVGTILTVLVHSSSASTAIVITLALEGVVDF
                     RMAAGLVLGCNIGTTIDAFLASLAARVHARRAAWAHILFNVAGTVWAVILFNPFLRLV
                     EVLTPGGTEGNVATHIAMLHTVFNVVNTLVLFPFVRQFAALLERLVRERPGEAAAPER
                     LRYVAGPIMDSPEMNLVYAQKEIADMAALARDMFVRFRNVLKSPPADLEPEIEAIAAL
                     EDRADQMREELTGFILECVSHEMGERARRDLAASLRIVDDLENITDDSLSLMHLMDRG
                     RKRKLPTDALKLEELGPYTLLVENFLGFVVANMNKHISEEQLAQASEYEDRIDGLRAT
                     LKKSARKRLKAGADVKLELLYIDLVRHVEKIGDHAYGIAHALREMR"
     gene            8777..>9352
                     /locus_tag="P5313_12655"
     CDS             8777..>9352
                     /locus_tag="P5313_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY81263.1"
                     /translation="MALSFGRTLIVDLGTRKVRIEPLEASEIRSYIGGSGIGTRLLAR
                     HVPPAADPLGPDNAVVFTVGPFCGTPVPTSGRHQVLAKSPLTGVFGECDVGGAWGSAF
                     RATGFDALVVRGISDRPVSLIVREDGASLEDAGDLWGRDTFETYETYTSRIPGSETAC
                     IGPAGERLVRIAAVLHDGRHARAAGRCGIGAV"
BASE COUNT         1432 a         3361 c         3359 g         1200 t
ORIGIN      
        1 ccccgggacc cgatcacttg ttcacctccc ggaacacgag ctccggtccg tcctcgagcg
       61 cctctggatc ctgaaccccg gcgccctacc ccggcggggt tcaggatcca gaggcgctcg
      121 aggacggacc ggagctcggc cttccggccc ctgcgggcca ggatctccgc caggcctccc
      181 aggccttcct ccgtcaggtc ccgctcccac gcgggatcca gggtcgccag ggccgcctcg
      241 acggcccggg gattccgcgc cagcagggcc gcttccaggt cgttggaagg ccgggcttcc
      301 ggcacccagg cttcctccag ggcccgggcc ctgcccaggt accgggcggc ccggcgcggg
      361 tatccccgga aggcgaggta ggcgtggacc gcggcgggaa gctccgcccc ctcccgggcg
      421 taggcctccg ccactgcccg ggcgtacttc cggaagaggc cctcgtgcca ggccgcgcga
      481 agggattcga cggcgcggct cgcccaggat gcggccccgt cccggagatc ccgcttcggc
      541 cgcgccttct cgacccgggc ccggccccgg tggatctccg acagcagttc gtggaggtcc
      601 atggcgaagc tgtcgggatc cgtaccgaag ctggccatcc aggggtggtc ctccaggtcc
      661 gcgacctccc ggacccaggc cagggcctcg tccagccggc ccgccgcaag ggccgactcc
      721 gccaggccga gacgtgccag gggggtcgtg tccactccga aggcgctggc gaagtccgcc
      781 tgggccgcgt ccagttccat ccgccggagc cggagctccc cccgggccag atagccggaa
      841 gaccgcgact ccgccagcag cgaccggtcc gccgcctcca gggcccgggc ccaaagccgg
      901 aatcgggact ccaggaggga caggttgtac agggccaccg ccgtgaacct cgagtagccc
      961 tccgcccggg cccccgcgat ggcttcctcg taccgccttc gggactcttc gtattcgaag
     1021 agctcagccc ggagagtccc cagggtcatg gaaaatccga tgtccggtcc caggcgccgc
     1081 tcggcgtcct ccaggagggc gatcccctca ttcaggcggt gggtcttgaa gagcatccac
     1141 ccgagatccc cgacggcccg ggtgcttccg ggatccaggt ccaggagcct ccgcaggtag
     1201 cggatggaat cctcctcccg gttcaggtac ccgtaggaat ccgcgatccg ctggagggtg
     1261 cccgtgtcgg ccgggtccag gctctccgcg gccaggtact cgtccagggc cagggcgtgg
     1321 agggaccggt ccgtgtagag gtcccccagg aggaccgccg ggcggggatc cccgggaaag
     1381 agcgagcggg cccggcgcag tatcgccgcg gctccgccgt agtcccccgc ctccgcggct
     1441 gcggcggctt cggcctccag ggcttgcagg tccggcgcct gggcggaggc cgcggcggcg
     1501 aaggacgccg ccaggaagac cgccaggacc cgcgccggga tgccgggttt ccgcctcctc
     1561 ctctccgtca tctccgccct ccccgccggc ccggaccccc cggcaggggc cgcaggccac
     1621 ggcgcaggtc caggaaggcc aatacccgtc gcggcgcggg gatccccgac cggactcggc
     1681 cgcggaagcc ccggatccgg gcctccaggt cccggacctc ctcggagttc ggggcgggat
     1741 cgtggaccgc cctctcccag tcccgggcga gcccttccag gtcttccgcc gaagtgccgc
     1801 ggccctcccg gtccaggcgc cccgcgaaat ccacgatcga ctccccccgg gaccggtgct
     1861 tcccggccga ggacagggca tcctccaagg actcccaggc cgccaggagc cttccgcggc
     1921 ggtccttgag gaggccgagg cgcagggccc ggatccctcg gcccaggacg gccagggcca
     1981 gccacagccc gggggccagg agccaggacc gccgaagaac ccaggcggcc gcccgccgga
     2041 gatcccaggc ggggcgccgg tccgaggcgg gccccgggcc ttccggggtc gccgcctccc
     2101 gggcaccccg gaggatctcc cgcaggaggc cttccagcaa ggccgggtcc gtgcccctgg
     2161 attcctcgaa gctctccccc ggggcgggct ctcggctggt ggggtcgaag gcgatccagc
     2221 cgtactcccc gaaccagacc tccgtccagg cgtgggccat gtccgcccgg acggggtgga
     2281 actccaggac cgccgtgtcg gggtccagga agaagcccgc cgccacccgg gccgggatcc
     2341 ccagggaccg gagcatcacg gtcatcgaga aggcgaagta cgagcagtac cccttccggc
     2401 tccggaagag gaagtgggac agctggttcc cgtccggggc cgccccgggt ttcagggagt
     2461 agcggtagtt ctcccggagg taggaaagca ccgccgtcac ccggtcgtac ggggtcgcgg
     2521 cctccgccgt cacctcctcg gccagggccc ggatccgcgg gtcgtccccc caccgggtat
     2581 accggtctcg gtcggattcg gagagggcga aggccggggg ggttcgggcc ggatcgaagt
     2641 ccccgaggag gagggagagg gccggcaccg ccgcccgtga gctcagggcg tacgcggagg
     2701 cgaaggagga gcccggctcc agcttccagg gaacgacgga taccggggcg tccaccccca
     2761 gcagggcccc gggctcgagg ttgaggatcc agtactcctg ctccagggat tgccggccct
     2821 cccagggcgg gacctcccag gagccgggtt cccggggcag tatttcgggg atcccttcat
     2881 ccccggggcc gggccggaac ccgccccggc ggtcgtactc cgagagcacg aagcgccgca
     2941 ccaggacgtt cgggtccacg ggatccttcc gggcgatcag gaccaggtcg tcggagaggg
     3001 agatctccgt ctccagcttg aggtaccggg ccaggtcgaa gcggaacagg gtcggcttca
     3061 agaggccccc gccccgggag gccgccccct cggagtaccg gcccaggagg gccaggagga
     3121 ccagggccag ggcggcgaac aggaccgcca accggaacgg gaagcccgga ctcctgcccg
     3181 agccccgggg cgcgaggagg gccaggacgg ccaggctcag gaagagcagg gccgcggcga
     3241 ccgcggccaa aaggctgggg tgggagtaca gagtgacccg gaaccctccc tgggacgcga
     3301 agaggagggc caggagggcg aggttcagcc cggcctcgaa gaccgcgaag gaccggttcc
     3361 gctccgccgc gtccgaggcg acgacgctcc agatccacag cggcaggagg ggacggaagg
     3421 tctcgtcgaa ggccgggaag agaccgtccg ttccgagacc cggaaggatc gcgccggcga
     3481 tcccgaaggc cgcgaagaac gcgagccgga gggcgaaggg caggactccg gccgcggcca
     3541 ggcggaccca gggccgcagg cgggtacgac ccagaagaac ccccgcggcg ctcaagagca
     3601 ggcagagccc cagggcccgg ggcagggata ccaggtccgc caggaggagc cgggcctggt
     3661 atgtcaggag gaggagggcc agggtacgga agatccgggc cgggacggac agaaccctag
     3721 aggacccgga catcgaagcc ccccgccccc gatagaaccc gggtctcccg atccagggcc
     3781 gtccggaacg cggcccggag ggcgcgcccg gtctccgggc catgcccggg agcgcgcggc
     3841 acccccgaag cgccggactt cgccggccgg ccgtccggga ccaggaagaa ccgggcccag
     3901 aagccggcgg gtccggtttc ggggaggtcc gggatgatca ggaccgtgtc gaagcccgcc
     3961 gccttccgct ccgccagcac ggccccgcgg cgaacggccc cgggagcgcc gaacaccgcg
     4021 agggggtcgg gacccggatt cactgccccc gccccgtcgg cgtcccaggc caggtccgcc
     4081 agccagtaca acctgcccgg gggatccggg ggggcgtcgg gagagcccgg cgcggagagc
     4141 cggacctcca agccccggtc ctccaggtac cgggcgtagc ccagggccgc ggatacgatc
     4201 ctgtccaggt agtagtcccc ggcctcgccg gccagccagg ccgggcgctc cgtgtccagg
     4261 aggatcctga cggtcttgcg gggcggaggg gagaggtctc cgggacgcac gaagaggtcc
     4321 ccgaagcggg cgtaaagctt ccagtggagg cgccgcgggt cgtccccggg caggtagggc
     4381 cgggagtcga agcggtcctc cccccgtatc ctccggtccg aaacgtcggc ccgctgtccc
     4441 cccgatcccc gggggacggc cgcggcggcg gccgagggct ccgggagcgc ggcggtctcc
     4501 aggattccgg gcagttcccg gcgggagtgc cagaacccga aggcgtcccc cgcgaccgcg
     4561 gcggcccggc cccggtacac cccccgggcc agagggccgg aaggcgggaa tcccgccgcc
     4621 ccggacgagc cgggcgcggg cagggccgcg gcggcttcgg cgtgccgccc ctgccggctc
     4681 ccgcaccgca gctccaccca ggccgagatc cccggtgccc ggggaagggg cactccgcgg
     4741 ttctcgatcc ggaagagggc ccgcaggtcc ggatccccgt cccgggacgg gggcgaaagg
     4801 ctcggccgga gggcctcggg gtcggcgccc agggcccggt cccgccggcg tccttccagg
     4861 agtactccgg ccagggaggc caggaccgcc gcggcaaggc ccgccccgga gatcagggcg
     4921 cccagctcgg acctcaggat tccggcggcc aggagccccg tggacaagag cgagaagccc
     4981 agcccgcggc gcgtgaggcg cggtcccgcg cccggaacgc ttcggcgtct tccgcgcgcc
     5041 tcgggattcc gcatggcccg cctaggtcct tcggagggga tccgtccggg cggccgcctc
     5101 ctcccggagc catcgggccg cgtcgaaccc gggatcccgg gtccggagcc ggtgggccaa
     5161 caccggggcg gccaggtcca cgatgtcctg ggggaccacg aagccccggc cctcctgcag
     5221 ggcccgggca cgcgccgcgg ccagaaggcc cagggccgcc cggggagacg cccccaggag
     5281 gatacccggg tgcgagcggg tcacggagac caggtccgcc agggagccca tgagtcccgg
     5341 atccgcccag accgcccggg cggtccggcg gacgaacagg agctcctcca cggagcgtcg
     5401 gggggaaagt ccggtcagca cattgaccga ggggtcgtcc cggaggatgg agatctccgc
     5461 gtcccggtcg ggatacccca gggagaccct catcaggaaa cggtccagct gggcggccgg
     5521 cagggggtag gttccctcgg tctccacggg attctgggtg gcggccacga agaagaactc
     5581 cggcaggggg cgggtcgcct ccgcggtggt gacctggccc tcggccatgg cctccaggag
     5641 ggcggactgg accttgggag gggtgcggtt gagctcgtcc gccaggagga cgttggcgaa
     5701 gacgggaccc gggtggaaga cgaaggcccg ggcgtcgggg tcgaagacgt ccacccccgt
     5761 cacgtcgtaa ggaaggaggt ccggggtgca ctggaggcgg cggaagaggg cgggggagtc
     5821 gggatcccgg gagagcaggg ccgcgaaggt cttggccgcg gtggtcttcc cgaggcccgg
     5881 aaggtcctcc accaggaggt gtccccccgc ggcggcgcag gccaggaagt cccgcacgaa
     5941 gccgtccttg ccccggagga ccgtgcccat cccggcggcc aggtccgcca ggatctccgc
     6001 cgaggcgcgc acgtccagga ccgacggcgt ctctcccccg atttggttca tggatccact
     6061 atagcacggc ccggggaggg gcacaaacgc ggccgccccg gcggaggcac cggaggaagc
     6121 gggggcggaa ggctacaagg tcttccaggc ctcctcgacg ttccgggaga accagcggaa
     6181 gacggcctcc gcccgattcc cgaaggagtc ctccagcttt ccccgcaccc cgggtaccac
     6241 cgcggcccgt gcgctggaca cggcccagaa caggatcccc tcgtcggtgt tcaggaccag
     6301 cacccggacc ttcagggccc cgggagcggc catgggtacg aacttgtagt acatccgggt
     6361 gaggttgacg gattccagga ggaccccgtc aggagcggtc cgatagtcca ggcggttcag
     6421 gttccccccg aaggagaggt ccttctgcca gaccgtgaag gtctcagccg gcggcacgac
     6481 cgagacccgc tggtcgggca cccgggtgcg gtcctccagg gagacgaccc gccaggactc
     6541 ctcgtagaag agacgcatgg tgttgcggct ggcggaccag tactcgatgc cctggagggt
     6601 cccgacggcc cggaggacgt tgtagaccgt caggagttcc gtcgcggggg cggcggagcg
     6661 cggttttttc cataggtaga gcgcctccac gaccacgtcc ggcccctccg cccgcaggtc
     6721 ggactggacc cctgcggcca gggggtggga gggcgaccag gcgacggcgg cctcccgacc
     6781 ggtggaggag cggcggagtt cctccccgcg gatcaggacg gcggcacggt ccgcgggcac
     6841 gcgggcggcc aaggggtccg cggccagggg caggacggcc gcgaaaaagg ctagggcggc
     6901 gatggccgcg ggggacgggg ttccggtccg catggtgtca ctcctggatg tagatccggc
     6961 ggccgcttcc tgccgcctcg aaggggaata taccgcggaa cggggcgctt cgccttcccc
     7021 ggcacgggcc cgggtccgct atgcaaaaac ctcgttagaa ttgtataagg ttcctgatcg
     7081 atccccgaat ttctaacgcg ggagcaccca tgaccgtcgt caccctgacc ttggaaatga
     7141 ttggcgccct gggattattc ctgtacggga tgaaggtcct gtcggacggg atccaacgcg
     7201 ccgccgggga ccgcctgaag ggcgccctga accagatgac ccggagcccc ctggccgcgg
     7261 tcctgacggg cctcggcgtg acgtccctgg tccagtcctc ctcggccacc acggtcatgg
     7321 tcgtctcctt cgtgaacgcg gggctcctct ccctgatcca ggccgtgggc gtcatcatgg
     7381 gggccaacat cggcaccacc gtgaccgcct ggatcgtctc cctggtgggc ttcaagttca
     7441 agatcaccct cctggccgtc cccctggtcg gggtgggctt catcctaacc acgtacctga
     7501 agacccggaa caagtccctg cggagctacg gggacgccct gatcggcttc gggatcctct
     7561 tcatcggcct ggacttcctc tcccacaccc tgcccaaacc cagccccgag gtccttcatt
     7621 tcctggcccg gttctccggc ctgggcctgg tctcggtcct gatcagcgtc ctggtgggca
     7681 cgatcctgac ggtcctggtc cactcctcca gcgcctccac cgccatcgtg atcaccctgg
     7741 ccctggaggg ggtcgtggac ttccggatgg ccgcgggcct ggtcctgggc tgcaacatcg
     7801 ggaccaccat cgacgccttc ctggcctccc tggccgcccg ggtccacgcc cgaagggccg
     7861 cctgggcgca catcctgttc aacgtcgccg gcacagtctg ggccgtgatc ctcttcaatc
     7921 ccttcctgcg cctggtggag gtcctgaccc cgggaggcac ggaggggaac gtggcgaccc
     7981 acatcgccat gctccacacg gtgttcaacg tcgtgaacac cctggtcctc ttccccttcg
     8041 tgcggcagtt cgccgccctc ctggagcgcc ttgtccggga gcggccgggc gaggccgccg
     8101 ccccggaacg cctgcgctac gtggccggac ccatcatgga ctccccggag atgaacctgg
     8161 tctacgccca gaaggagatc gccgacatgg ccgccctggc ccgggacatg ttcgtccgct
     8221 tccggaacgt cctgaagtcc ccgccggcgg acctggaacc cgagatcgag gccatcgccg
     8281 ccctggagga ccgggcggac cagatgcggg aggagctgac cggcttcatc ctggagtgcg
     8341 tgtcccacga gatgggcgag cgggcccgca gggacctggc cgcctccctg cggatcgtgg
     8401 acgacctgga gaacatcacg gatgactccc tgagcctcat gcacctgatg gaccggggga
     8461 ggaagagaaa gcttcccacc gacgccctga agctcgagga gctgggcccc tacaccctcc
     8521 tcgtggaaaa cttcctgggt ttcgtggtgg ccaacatgaa caagcacatc tccgaggaac
     8581 agctggccca ggcctcggag tacgaggacc gcatcgacgg gctccgggcc accttgaaga
     8641 agagcgcccg aaaacgcctg aaggccgggg cggacgtgaa gctcgagctc ctctatatcg
     8701 acctggtccg gcacgtggag aagatcggcg accacgccta cgggatcgcc cacgccctgc
     8761 gggaaatgag gtaaccatgg cactttcgtt cggcaggacc ctgatcgtcg acctgggcac
     8821 ccggaaggtc cggatcgaac cgttggaggc ttcggagata cggtcctaca tcggcggctc
     8881 cggaatagga acccggctgc ttgcgcgcca cgtccctccg gccgcggatc ccctgggacc
     8941 ggacaatgcc gtggtcttca ccgtcggacc cttctgcggg acccccgtcc ccacctcggg
     9001 ccggcaccag gtcctggcca agagtcctct cacgggagtc ttcggggaat gcgacgtcgg
     9061 gggagcctgg ggctccgcct tccgggcgac gggcttcgac gcgcttgtgg tccggggaat
     9121 ctccgaccgg cccgtctccc tgatcgtgcg ggaggacggc gcctccctcg aggacgccgg
     9181 ggacctctgg ggccgggaca ccttcgagac ctatgaaacc tatacgtccc gcattcccgg
     9241 gtccgagacg gcctgcatcg gccccgcggg ggaacgcctg gtccgcatcg ccgcggtcct
     9301 ccacgacggc cggcatgccc gggccgcggg ccgctgcggg atcggggccg tg
//
LOCUS       DAOTEI010000144         9199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5342_length_9199_cov_4.396325, whole genome shotgun sequence.
ACCESSION   DAOTEI010000144 DAOTEI010000000
VERSION     DAOTEI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5342_length_9199_cov_4.396325"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="P5313_12660"
     CDS             <1..314
                     /locus_tag="P5313_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRY81264.1"
                     /translation="SQYKNKDVAMSVLKSRLYEHYRQEKEKENAKFASEKKEIAWGSQ
                     IRSYVFQPYTMVKDHRTKFSVGNVQGVMDGDIDPFIENWLQFSWAGGSPAPEGGDDDE
                     D"
     gene            325..1737
                     /locus_tag="P5313_12665"
     CDS             325..1737
                     /locus_tag="P5313_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81265.1"
                     /translation="MTPGRFGALALLLASASFPASGQGEAPAWILGVASFPPAAREVR
                     LYPALGQLPVQVLEGVRDLDRRFLTEEERTRILEKSRLEELFKAGAAAAGTRDRRDAA
                     ALSPVTGPARAAEERRLGEELARGPKTGSDPTAGVSSPGGPSPGVSSPDIRRVVFWEG
                     HSSGRLIEAGADPGSVCSREKLDYLVLWEAGEVSGYLRIRMTGWNAVLSRPDFTYTAY
                     CPVDDIPAAAAELSRGLVRAAAGVPSSRLVFTVDPPESRIRVDGRLLAPGRRSLRVYE
                     EREHLVSLELGAGTREEYSVRSEFGKDVELSVRLEPPDPETVLVETDPPGASLHLDGI
                     WAGTTPGPALVFGSARVARLTLPGFQDEYRVIEPGTEGPVLVPLREAETGGTSLFERR
                     KERFYRALGGLAVSLPVTLLSYGVYLQSSALHMEYPADEAFARRKDTSLAVFAISTGI
                     TAGLLTGAAVRLVKYIQSAR"
     gene            1792..2625
                     /gene="ftsY"
                     /locus_tag="P5313_12670"
     CDS             1792..2625
                     /gene="ftsY"
                     /locus_tag="P5313_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRY81266.1"
                     /translation="MGPGDEFYECLEDLLIEGDLGARAALEAVSELRGHCRREGILDE
                     SGIRRALKDVLLRYGKEVRIEPSAGGLDVILLLGVNGVGKTTTAAKLAHWYREQGLAR
                     NIILAAGDTFRAAAIDQLKVHGERLGLRVVAQQPGSDPGAVLYDAIEAAKADGADLVL
                     ADTAGRMHTKQNLIRELEKLDRIVGARAQPSRYRKLLVVDGTTGQNALRQAEVFGEAV
                     RLDGAVLTKYDSSGKGGIVISMAYERGIPTAFLGTGEGYGDIKPFRLGEYLDDLLGGP
                     G"
     gene            2652..3443
                     /locus_tag="P5313_12675"
     CDS             2652..3443
                     /locus_tag="P5313_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81267.1"
                     /translation="MPLLLLAAPGLRAQESAPAAPEEIFRYVLAAGTVTGAVVTTPEG
                     VELRRETYIRDSEGRVVDVRISLPDGRSVRTGGSGGREWLGYPEGDLSFRVYRPDGVL
                     ELEESRNGSILLFRRTYTYRGDSRSPSVVEEDRPGEGWKRRTEYGERGLPARESLSTA
                     DGPETVTLYEWDERDRPVGLRILEGRSERRVRFSYGEDGTETEERTDSTGALVLRILR
                     NPDGSSVEERFDGGILFARTFLKDGRLTREEIYREGRLARVRTAP"
     gene            3440..4747
                     /locus_tag="P5313_12680"
     CDS             3440..4747
                     /locus_tag="P5313_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81268.1"
                     /translation="MKGTASWAAFVALRYFRARRVKGGAASFLLAALGIAVGTGTLVV
                     VLGVMNGFQSGFIDSILEVDSHHVRISLAAEDPSGQEPGLLADQLREDPAVSAALPFA
                     DFETVALGPSGRAYPLRVKALPEDAGTRDPALTDRLGLGSAPFPGSSPGGIVVGAELA
                     RQLDVLPGDEVSLLTVRASAEEGVEASTVRVRVTEVFHSGYYAFDYGLGFVTFETSRA
                     FGGPVNLVLGVKLKDRFGDEGFAERLPSRYGLPVDRIVTWRTYNRSFFGALRMEKTAM
                     MLLVGLIFLVVAVNIFHSLRRTVFEKMEDIAVLKALGGSARTVRSIFVLDGLVTGFLG
                     ATCGLAAGLAVTMNVNEVFAAAEALVNAVLRLGSALSRAGGSGDFQVFSPAYFYLVRI
                     PARVVFEEILFIYGAGASSAAAAAWAASRRVLAFRPAEVLRNE"
     gene            4740..5420
                     /locus_tag="P5313_12685"
     CDS             4740..5420
                     /locus_tag="P5313_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81269.1"
                     /translation="MSEAIIVCEDLSKTFKTDAEELHILRSVDLTVEAGSTASITGPS
                     GCGKTTLLSILGGLDSPGGGRVRVGPWNLHELGEDGLSEYRSSFVGFVFQFHYLLKDF
                     TALENVALPAYMRGLSRAEAFDRAAALLADLGLGDRKNQYPGKLSGGERQRAAIARAL
                     VNRPSVILADEPTGNLDEANARAVEDLLLGLAPGYGATLVVVTHDARFASRTDQRFRL
                     TEGEIEAP"
     gene            5417..6745
                     /locus_tag="P5313_12690"
     CDS             5417..6745
                     /locus_tag="P5313_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81270.1"
                     /translation="MKAGPVLFLALRNILGRRSDERDLARRTLRGAVLSVAVSMVPLI
                     VTLVVADGMIQGITSRYIELSSYHIQCVPFRGQGESSVRDALSALDGLPGVRGAWAET
                     QAVGVAFSKGRRGGALVRAVDPGFLADEGTRSYLGILSGEAALSGPNDVILGSELARD
                     LGAEPGSTVSLISVRSRRGDTIVPRVSVFRVRGIVSSGYRDLDSRWFLVPRDSSGRYL
                     TSDTSRTILGVKVADPFAPLDIVVERIRAALPEGWAVQPWPQTERNLYESFRTTRYLL
                     LLIMGLTVAVAAVNISSALVTLVTERSHEIAILKGLGATPKDIGRVFVAGGMILGFAG
                     SLLGTALGILAAVHVNELIASLERVLAGASALFHALAWPFLDESARALPAPVVRILNP
                     DYYLESIPVVLDYRALGGILAAALFLSYLASLLPARKAAALSPLEILRRR"
     gene            complement(6781..8400)
                     /locus_tag="P5313_12695"
     CDS             complement(6781..8400)
                     /locus_tag="P5313_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006209983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRY81271.1"
                     /translation="MDFDPNRRPYPSLRYPIYANRGMVAASSPQASAAGLQALRSGGN
                     AVDAAVAAAATLTVTEPTANGIGGDAFALVWIEKDRRLYGLNASGRAPSGISIEAVLA
                     RNPPGERMPALGWLPVLVPGIPKAWASLVRRFGRRSLGEALEPAAEYAEGGYPAGPVL
                     AGAWQRSFDRYSRECRGPEFAEWFRVFAPEGRPPAPGRIVRLPDHARTLRSLGASDSE
                     SFYRGDLADRIVRDSEEFGGFLRKPDLEGYEAEWVDPISVAYRGYEVCEIPPNGQGIV
                     ALMALNILKGFPAAREPSPEDYHRSWEAMKLAFADGTAHVTDPGSMSVFPRDLLSPDY
                     GALRAREIGEAARIHPPAAPGRSGTVYLCTADGNGNMVSYIQSNYLGFGSGVVVRGTG
                     IALQNRGFDFSLDPRHVNRLEPGKRTYHTIIPGFLMKDGRAVGPFGVMGGYMQPQGHV
                     QVVRNLLDYGLDPQQALDAPRWQWTEGLRFLVEEGFPREAAEELARRGHEVAVSRDTA
                     TFGRGQIILRLPGGTLVGGTEGRTDSNIACW"
     gene            8545..>9199
                     /gene="recA"
                     /locus_tag="P5313_12700"
     CDS             8545..>9199
                     /gene="recA"
                     /locus_tag="P5313_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRY81272.1"
                     /translation="MARNDAEGAPGDAGEKLKALEAARLQIEKQFGLGSLMKLGTHAS
                     AQGVEVIPSGSILLDEALGVGGYPRGRIVEIYGPESSGKTTLALHVIAEAQKLGGIAA
                     FIDAEHALDPIYARNLGVNIDELWVSQPDNGEQALEIAESLVRSGAVDVIVVDSVAAL
                     TPQAEIEGDMGDAHVGLQARLMSQALRKLTATIGKSKTILVFINQIRMKIGVMFGNPE
                     "
BASE COUNT         1398 a         3065 c         3269 g         1467 t
ORIGIN      
        1 ggagccagta caagaacaag gacgtcgcga tgtccgtgct caagtcgcgg ctctatgagc
       61 actaccgcca ggagaaagag aaggagaacg cgaagttcgc ctcggagaag aaggagatcg
      121 cctggggaag ccagatccgc tcctacgtct tccagcccta caccatggtg aaggatcacc
      181 gcaccaagtt ttccgtcggg aacgtgcagg gcgtcatgga cggggacatc gatcccttca
      241 tcgagaactg gctccagttc tcctgggccg gagggtctcc ggctccggag ggcggggacg
      301 acgacgagga ttgacgggtc cgccatgacc cccgggagat tcggagccct cgcgctcctg
      361 ctggcctccg cgtccttccc ggcttcgggt cagggggaag ctccggcctg gatcctcggg
      421 gtggcctcct tcccccccgc ggcccgggaa gtccggctgt atcccgccct cggccagctg
      481 ccggtccagg tgttagaagg cgtccgggac ctggacagga ggttcctgac cgaggaggaa
      541 agaacccgga tcctggagaa gtcccggctg gaggaactgt tcaaggcggg ggccgcggcc
      601 gcggggaccc gggatcgaag ggacgccgcg gccctttctc ccgtgacggg ccccgcgcgg
      661 gccgcggagg agaggaggct cggagaggag cttgcgcgag gcccaaaaac cggctccgac
      721 ccgacagcgg gcgtatcctc gccgggcgga ccttcgccgg gcgtatcctc gccggatatc
      781 cgcagagtgg ttttctggga gggacactcc tcgggacgct tgatcgaggc cggggcggat
      841 cccggaagcg tctgttcccg ggagaaactc gactacctgg tgctctggga agccggggag
      901 gtctccggat atctgcgtat ccgcatgacc ggatggaacg cggtcctgag ccgcccggac
      961 ttcacctata ccgcctactg cccggtggac gacatcccgg ccgcggcggc ggaactttcc
     1021 cgggggctcg tccgggcggc tgccggagtg ccgtcctccc ggctcgtctt caccgtggac
     1081 cccccggagt cccggatccg ggtggacggc cgccttctgg ctcccgggcg ccggagcctt
     1141 cgggtctacg aggaacggga gcatctcgta tccttggagc tcggggccgg gacccgggag
     1201 gaatacagcg tccggtccga gttcggcaag gatgtggaac tctccgtccg cctggagccc
     1261 ccggaccccg agacggtcct cgtggagacg gaccccccgg gggcttccct gcacctggac
     1321 gggatctggg ccggcacgac ccccggcccg gcccttgtct tcggatccgc ccgggtagcc
     1381 cgcctgacgc ttccggggtt ccaggacgaa taccgtgtga tcgagccggg aacggaagga
     1441 cccgtgctcg tgccgctccg ggaggcggag accggcggta cttccctctt cgagcggcgg
     1501 aaggaacggt tctatcgggc cctgggcggc ctcgcggtca gcctgcccgt gaccctcctg
     1561 tcctacggtg tctacctcca gagttcggcc ctgcacatgg agtatcccgc ggacgaggcc
     1621 ttcgcccgaa ggaaggacac gtctctcgcg gtattcgcca tttcgaccgg gatcacggcg
     1681 ggcctgctga cgggcgccgc ggtccggctc gtgaagtata ttcaatcggc gcgatgagga
     1741 ggtcccggag tgggagcccg tttttccgat cggcttaagg cgttgctggg catgggcccg
     1801 ggagacgagt tctacgaatg cctcgaggac ctcctgatcg agggggatct gggagcccgc
     1861 gcggcgttgg aggcggtcag cgagctccgg ggacactgcc gccgggaagg gatcctggac
     1921 gaatcgggta tccggcgggc cctgaaggac gtgcttctcc ggtacggcaa ggaagtccgg
     1981 atcgagccct ccgcgggcgg actggacgtc atcctgctcc tgggggtgaa cggggtggga
     2041 aagaccacca ccgcggccaa gctggcccac tggtaccggg aacaggggct ggcacggaac
     2101 atcatcctgg ccgcgggcga caccttccgg gccgcggcca tcgaccagct caaggtccac
     2161 ggcgagcgcc tgggcctgcg ggtggtggcc cagcagccgg gctccgaccc cggagccgtg
     2221 ctctacgacg ccatcgaggc cgccaaggcg gacggtgccg acctggtcct ggccgacacg
     2281 gcgggccgca tgcacacgaa gcagaatctc atccgggagc tcgagaagtt ggaccggatc
     2341 gtcggagccc gggcccagcc gtcccggtac cggaagctcc tggtggtcga cggcaccacg
     2401 ggccagaacg ccctacgaca ggcggaggtc ttcggagagg cggtgcgcct ggacggggcg
     2461 gtcctgacca agtacgattc ctccggcaag ggggggatcg tgatctcgat ggcctacgaa
     2521 cgggggatac ctacggcctt cctgggcacc ggggaggggt acggggacat caagcccttc
     2581 cggctggggg aatacctgga cgacctcctg ggaggtccgg gctgagcctc cggcgcctcc
     2641 gcgcgtcctg ggtgccgctt cttctgttgg cggcgcccgg tctccgcgcc caggagtccg
     2701 cccccgcggc cccggaggag atttttcggt atgtccttgc cgccggcacg gtcacgggag
     2761 ccgtggtcac gactcccgag ggcgtcgaac tccggcggga gacctacatc cgggactcgg
     2821 aaggacgggt ggtcgatgtc cggatctcct tacccgacgg ccgctcggtg cggaccggag
     2881 gctccggcgg ccgggaatgg ctcgggtatc ccgaggggga cctctccttc cgcgtgtacc
     2941 ggccggacgg ggtcctggag ctcgaggaat caaggaacgg atcgatcctg ctcttccgaa
     3001 ggacctacac ctaccgtggg gattcccgct cgccctcggt cgtggaggag gaccggcccg
     3061 gggagggctg gaaacgcagg accgagtacg gcgagcgggg tctccctgcg agggagagcc
     3121 tctccacggc cgacggaccc gagacggtca ccctctacga atgggacgaa cgggaccggc
     3181 cggtcggcct ccgcatcctg gagggccgga gcgagcggcg ggtccgcttc tcctacggcg
     3241 aggacgggac cgagacggag gaacgcacgg actccacggg tgccctggtc ctgaggatcc
     3301 ttcggaaccc cgacggaagc tccgtggagg agaggttcga cggcgggatc ctgttcgccc
     3361 ggaccttcct gaaggacggc cgcctgaccc gtgaggagat ctacagggaa ggtcggctgg
     3421 cccgggttcg gacggctcca tgaaagggac agcctcctgg gcggccttcg tggccctccg
     3481 gtatttccgc gcccgtagag tcaagggcgg agccgcgtcc ttcctcctgg cggccctggg
     3541 gattgcggtg gggacgggaa ccctggtcgt ggtcctgggg gtgatgaacg ggttccagtc
     3601 gggcttcatc gattcgatcc tggaggtgga cagccaccac gtccggatct ccctggccgc
     3661 ggaggatccc tccggacagg aaccggggct ccttgcggac cagctccgcg aggacccggc
     3721 ggtttccgcg gccctcccgt tcgcggactt cgagaccgtg gccctgggtc cctccggcag
     3781 ggcatacccg ctccgcgtca aggccctgcc cgaagacgcg gggacgcggg accccgccct
     3841 gacggatcga ctcggtctgg gctccgcacc gtttccgggt tcctctcccg gcggcatcgt
     3901 ggtgggcgcg gagctggccc gccagctgga tgtccttccg ggggacgagg tcagcctcct
     3961 gaccgtccgc gcctccgccg aggagggggt cgaagcgtcc acggtccggg tgagggttac
     4021 ggaggtgttc cacagcggat actacgcctt cgattacggc ctgggattcg tgaccttcga
     4081 gacctcccgg gctttcggcg gccccgtgaa cctggtcctg ggcgtcaagc tcaaggaccg
     4141 cttcggcgac gaggggttcg cggaaaggct gccctcccga tacgggcttc ccgtggacag
     4201 gatcgtgacc tggaggacct acaaccggtc cttcttcgga gccctgcgca tggagaagac
     4261 ggccatgatg ctgttggtgg ggctcatctt cctggtcgtg gcggtaaaca tcttccattc
     4321 cctgcgccgg acggtcttcg agaagatgga ggacatcgcc gtcctgaagg ccctcggagg
     4381 ctccgcccgg acggttcgct ccatcttcgt cctggacggc ctggtgacgg ggtttctggg
     4441 ggcgacctgc ggcctggcgg ccggcctggc ggtcacgatg aacgtgaacg aggtattcgc
     4501 ggccgcggaa gccctggtga acgccgtact ccgtctgggc agcgccctat cccgggccgg
     4561 cggaagcggg gacttccagg tcttttcccc cgcgtatttc tacctggtcc gcatacctgc
     4621 ccgggtggta ttcgaggaga tcctgttcat ctacggcgcg ggagcttcca gcgcggccgc
     4681 ggccgcctgg gcggcgtccc gacgggtgct ggcgttccgg cccgcggagg tcctgaggaa
     4741 tgagtgaagc gatcatcgta tgcgaggact tgagcaagac cttcaagacc gacgcggagg
     4801 agcttcatat cctgcgctcc gtcgacctga ccgtggaggc cggttccacg gcctccatca
     4861 cagggcccag cggctgcggc aagacgaccc tcctgtccat cctggggggc ctggattccc
     4921 cggggggcgg ccgggtccgg gtggggccct ggaacctcca cgaactgggg gaggacgggc
     4981 tctcggaata ccgttcctcc ttcgtgggtt tcgtgttcca gttccactac ctgctcaagg
     5041 atttcacggc cctggagaac gtcgccctgc cggcctacat gcgcggcctg tcccgggcgg
     5101 aggccttcga ccgggccgcg gcgctccttg ctgacctggg cctgggggac cggaagaacc
     5161 agtaccccgg gaagctctcc gggggggaac gccagcgggc tgccatcgcc cgcgccctgg
     5221 tcaaccgtcc ctcggtgatc ctggcggacg agcccacggg caacctggac gaggcgaacg
     5281 cccgggccgt ggaggacctc ctcctgggcc tggcgcccgg gtacggcgcc accctcgtgg
     5341 tcgtgaccca cgacgcgcgc ttcgcctccc ggacggacca gcgtttccgc ctgaccgagg
     5401 gggagatcga agccccgtga aggccggccc cgtcctcttc ctggccctgc ggaacatcct
     5461 cggacgcagg agcgacgagc gggacctggc ccggcggacc ctgcgcgggg ccgtgctctc
     5521 cgtggcggtc agcatggtgc ccctcatcgt caccctggtg gtggcggacg ggatgatcca
     5581 gggcatcacg tcccggtaca tcgagctgtc ctcctaccat atccagtgcg taccgttccg
     5641 tggccagggg gagagttcgg tccgggacgc cctgtccgcc ctagacgggc tgcccggggt
     5701 tcggggcgcc tgggcggaga cccaggcggt gggggtggcc ttttcgaagg gccggcgcgg
     5761 cggggccctg gtgcgggcgg tggatcccgg attcctagcg gacgagggaa cccgttccta
     5821 cctggggatc ctgtcgggtg aggcggcgct gtccggtccc aacgacgtga tcctgggcag
     5881 cgaactggcc cgggacctgg gggcggagcc ggggagcacg gtgagcctga tcagcgtccg
     5941 gtcccggagg ggcgacacga tcgtcccccg ggtcagcgtc ttccgggtgc gcgggatcgt
     6001 ctcctccggg taccgggacc tggattcccg ctggttcctg gtgccccggg attcctcggg
     6061 ccgctacctt acttcggaca cctcccggac gatcctgggg gtcaaggtcg cggatccctt
     6121 cgctcccttg gacatcgtcg tcgagaggat ccgtgcagcg cttcccgagg ggtgggccgt
     6181 acagccctgg ccccagaccg agaggaacct ctacgagtcc ttccggacca cccgctacct
     6241 cctgctcctg atcatggggc tgaccgtggc ggtcgcggcg gtgaacatct ccagcgccct
     6301 ggtcaccctg gtgaccgaga gatcccacga aatcgccatc ctgaagggcc tgggagcgac
     6361 acccaaggac atcggaaggg tcttcgtggc cggcggaatg atcctgggtt tcgcgggatc
     6421 ccttttgggg accgccctgg ggatcctggc ggcggtgcac gtgaacgaac tgatcgcgtc
     6481 tctggagcgc gtcctggccg gggcgtcggc cctgttccac gccctcgcct ggcccttcct
     6541 ggacgagtcg gcccgggcgc ttccggctcc cgtggtcagg atcctgaatc ccgactacta
     6601 cctggagagc attcccgtgg tcctggacta ccgcgccttg gggggcattc tggcagcggc
     6661 actcttcctc tcctacttgg cctccctcct gcccgcacgg aaggccgcgg ccctatcccc
     6721 cctggagata ctccgcaggc ggtaggccgg gccggcgatc cctcccgtcg gacccggagc
     6781 tcaccagcag gcgatgttgc tgtccgtccg cccttcggtg ccgccgacca gagtgccccc
     6841 cggaagtcgc aggatgatct ggcctcggcc gaaggtggcg gtgtcccggg atacggccac
     6901 ctcgtggccc cgccgggcca gctcctccgc ggcctctcgc gggaagccct cctccacgag
     6961 gaagcgcaga ccctcggtcc actgccaccg gggggcatcc agggcctgct gggggtcgag
     7021 gccgtagtcc aggaggttcc gcacgacctg aacatggccc tggggctgca tgtagccccc
     7081 catcaccccg aagggaccga cggcgcggcc gtccttcatc aggaaaccgg ggatgatggt
     7141 gtggtaggtc cgttttcccg gctccagtcg gttgacgtgg cggggatcca gggaaaaatc
     7201 gaagccccgg ttctgcaagg cgatcccggt tcccctgacc acgacgccgg agccgaagcc
     7261 caagtagttg ctctggatgt aggaaaccat gttgccgttc ccgtcggcgg tgcagaggta
     7321 gaccgtgccg gaccgtccgg gggcggcggg gggatggatc cgtgcggcct ccccgatctc
     7381 ccgggcgcgc agggccccgt agtccgggga tagcaggtcc cgggggaaaa cgctcatgct
     7441 cccgggatcc gtgacatggg ccgtgccgtc cgcgaaggcc agcttcatgg cctcccagga
     7501 gcggtggtaa tcctccggcg agggctcccg ggccgccgga aaccccttca ggatgttcaa
     7561 ggccatcagg gccacgatgc cctggccgtt cggggggatc tcgcagacct cgtatccccg
     7621 gtacgcgacg gagatgggat cgacccactc ggcctcgtag ccttccaggt ccggcttgcg
     7681 gaggaaaccg ccgaactctt cggagtcccg tacgatacgg tccgcaaggt cgccccggta
     7741 gaaggattcg gaatccgagg ctcccaggga cctcagggtg cgggcgtggt ccgggaggcg
     7801 cacgatccgg cccggggcag gaggacgtcc ttcgggggcg aagacccgga accattcggc
     7861 gaactcgggc ccccggcatt cccgggaata ccggtcgaag gaacgttgcc aggctcccgc
     7921 aaggacgggg cctgcgggat aacccccctc ggcatattcc gccgcgggct ccagggcctc
     7981 ccccagggaa cggcggccga agcggcgtac cagggaagcc caggccttgg ggatcccggg
     8041 caccaggacg ggcagccatc ccagggccgg catcctttct cccgggggat tccgggccag
     8101 caccgcttcg atggaaatcc cggacggagc ccggcccgag gcgttgagtc cgtataggcg
     8161 acggtccttt tcgatccaga ccagggcgaa ggcgtccccg ccgatcccgt tggccgtcgg
     8221 ttcggtcacg gtcagggtcg ccgcggccgc caccgccgcg tccacggcgt tccctccgga
     8281 ccggagggcc tggagccccg cggcggaggc ctggggggag gaggcggcga ccatcccccg
     8341 gttcgcgtag atcgggtacc gcagggaagg gtagggacgg cggttcggat cgaagtccat
     8401 gctgcgtcct ccggacccgc ggatccgctt tcctagtcgg cgaatcccgt gtatagttga
     8461 gccgatcgaa aaagacggtc gaccgctgct ggcgaagcaa gcgccggggc ccggtcgagg
     8521 cggtttcggc atcaggagga taccatggca cgcaacgacg cggaaggagc cccgggggac
     8581 gcgggcgaga agctcaaggc cctggaggcg gcccgcctgc agatagagaa gcagttcgga
     8641 ctggggtccc tgatgaagct gggcacccac gccagcgccc agggggtgga ggtcatcccc
     8701 tccgggtcga tcctcctgga cgaggccctg ggcgtggggg gctacccgag gggccggatc
     8761 gtggagatct acggccccga atcctcgggc aagacaaccc tggccctcca cgtcatcgcg
     8821 gaggcccaga agctgggggg gatcgcggcc ttcatcgacg cggaacacgc cctggacccc
     8881 atctacgccc gaaacctggg cgtcaacatc gacgaactct gggtttccca gccggacaac
     8941 ggggagcagg ccctggagat cgcggaatcc ctggtgcgct ccggggccgt ggacgtcatc
     9001 gtggtggatt ccgtggccgc cctcaccccc caggccgaga tcgaggggga catgggggac
     9061 gcccacgtgg gcctgcaggc ccgtctgatg agccaggccc tgcggaagct gaccgccacc
     9121 ataggcaagt ccaagaccat cctggtcttc atcaaccaga tccgcatgaa gatcggcgtc
     9181 atgttcggca atcccgaga
//
LOCUS       DAOTEI010000145         9189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5352_length_9189_cov_4.335122, whole genome shotgun sequence.
ACCESSION   DAOTEI010000145 DAOTEI010000000
VERSION     DAOTEI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5352_length_9189_cov_4.335122"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="P5313_12705"
     CDS             <1..506
                     /locus_tag="P5313_12705"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81273.1"
                     /translation="CGQDPGIFRTDPGSRSMSTERTFGRDIADPETLESLLLGMAQEI
                     SQRAWREGVRSKTVFIKLRYADFRTLNPRRTSSSFFEESGVLWSEACRLFRSAWNGGP
                     VRLLGLGLGNLQDRKPESQGELFPAGDDRRARVEQAVFELERKGVGKVTRARLIGREG
                     DRIPGDP"
     gene            complement(555..1703)
                     /locus_tag="P5313_12710"
     CDS             complement(555..1703)
                     /locus_tag="P5313_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81274.1"
                     /translation="MKIGALVVVAACSILVCAGCVSTPAALEPGPGPKPGSGTVRPAS
                     AVDEMKRLLEVARTAQDSNHPAEAIRSYVTVLALSREDGSAEAARLGSQAEAELVRIG
                     NRLSLEPGEGWIGPDGSQAAAASRSVGKEGSLQPAVFLFENFAAAKSPVADAPIRFEF
                     VRGGGVLTTSVSTDIYGRANAILSRLDSPSSEAVVRAYPAFTVRGFTYVFRNVQRDFS
                     YLPPSNVAKVVALERGPEGAVQNPQVLDSVAAILKGAGLVPSPLDGKLLGSGFDRAFG
                     GDPASVRALAASAGASYFAFVVSEASPARQMEYGGKKYNIFTSDCKTTIRIIRDDGTI
                     VFALPLDPVRGQGGTASASVSDAFLKARDALTAELNRRVPEIKKAVEQ"
     gene            complement(1772..2056)
                     /locus_tag="P5313_12715"
     CDS             complement(1772..2056)
                     /locus_tag="P5313_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRY81275.1"
                     /translation="MVVSMIQFILELPETTSIKEKRKIVVSLKEKLRVKFRISCAEVD
                     LQDSLQWAQLGGALVSNSREHGELVLQKVLAFAEGQCRVRDVQIHSEVFE"
     gene            complement(2050..2697)
                     /locus_tag="P5313_12720"
     CDS             complement(2050..2697)
                     /locus_tag="P5313_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81276.1"
                     /translation="MKERSPGPLGILLAVSAAVSLAAWAFEEISVLAPALGPYSKWAS
                     LAGLALDACILGFGSALTALAALESGRSPRDSWIEVISSLPVIVFASAPLAYAHLEGR
                     PDAIPLVLLAVRSLRILRLRRILPESRPAILFILAFFELFRAFAQALLPDSISFSASL
                     RLLLLPEALLLLGAAALGAKGLRAFRSPGDPENGIADPVVGQDELAGLLGKRSSW"
     gene            2750..6820
                     /locus_tag="P5313_12725"
     CDS             2750..6820
                     /locus_tag="P5313_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81277.1"
                     /translation="MGKKSAVAAIFLLAALLAAAQSTEGTEAEPAPQEVPASGSAPTP
                     PPLAPVPVLPVEAPQSLLSLRMGDKDVELLAEGYWEVSVLASGAFGLGANEGVSAPTL
                     LFRQIPDLWLSLTLLDRWFVEASLSSEGFGDQYVAGYRGGEGDFLREVRLGNKGVSFP
                     DLPYLSLGAGSYTSFGISALGRSDRGSAHFLVRYDQARRITQTWVGSREVTRTEYRPS
                     EYVRGRWFMLPDQGVSDVELYIESSSGTIIGSDTRKYRTMTADEYSLSAATGRVDLKT
                     AVGEGRRLLVYYPGLNVLAITYDDGPPFYNEPPAGVTGVASGTALVLYERGEHEGFQI
                     ANRYALASSASAASAIAYVRDAATGLPDPAYTATVLSDGFARVTQTVAVTSAPDFSDY
                     DFRMPFAGPPLTTIDVGMDWLYDPDAVEAAEGTPTEPAPAYSRVLVVETYGAPGSLVL
                     EETGVLPGSVEVRRNGVLDYGFRYDPASNVVTLDRDPAISDIIEVSYLLASSDRNAGS
                     LAAGLAGIFDLSPDWRAWTALGLRWGVPGTGYSEGGDAVPGSLTLTAGIRGGSPEPDA
                     GPDAAGAPRFKAEAALAASYRRPDASGYYRLAGMEEASSWTSPFRPTGTWPAGVGVAT
                     VAAVADTAAPSLESLFPKLYGRFHSATSIQKVLQVSFAGHDGTTAEVVRYEEPVAVGD
                     YRAFAFFARRDFDAAGAVLTVALDGADPAISVQVPAGEMVETWRRFVVRYDGVAKLYY
                     QDTEDGPEHEIGVIPDPDPSVSAGRITISVTGVTDGSVFVDEFHFLDPAGEIALSGRG
                     SFSWSRGGTLAESGAGAILSDPGFSARLSGTISEDSNFAGYLDGRIAVGPAKLAANLR
                     QGYADGELSDGAFGHSLELPLKGFSVGDSFQFDSGSGRFGRRDFLSLQARGLGLDLEQ
                     SASYGGSVLEQAWKVRLAAGSWMTLGGELSNRAAGAVDLSSAYGEAWIEAFRYALPAL
                     EDASLRRTTTLTGAWKLGGGALTVAADSGLTDAVSPASAGSAGLNARLGWSIPAGRGS
                     VEPYYRRVWTAGAVSQSSAFPEDWEFWIDRFSSSEYLYRTIPFAELFDPFTAGLFEAA
                     AQGTTSSSYKPETGILFGRRYGSSWTDLVIPSKAALSLRRELASSLETVTDALVWEAS
                     ASMAAVNLFGSRGTYRFSRLYSVDEYSQRFAFSLTSYRGDPDPLASFSHTALASFYTT
                     REDNLVAENRLDLTESRDGLAWSERLSLNLTLRPSRTWLGDLVTLLLERSGSKPEPEE
                     GTGPTLVSGFWDSLRTARGRLSEVWGASFLVKRPYAYQDRFDVELAQSYETKYVVSQK
                     ASLWARLGTRQTLNILEGADYWMLGFELTLGARVIF"
     gene            6914..>9189
                     /gene="metG"
                     /locus_tag="P5313_12730"
     CDS             6914..>9189
                     /gene="metG"
                     /locus_tag="P5313_12730"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRY81278.1"
                     /translation="MKRRLITSALPYVNNIPHLGNLIQVLSADVFARFCRLRGYETLY
                     VCGTDEYGTATETKALEEGITPRELCDRFFVVHRDIYAWFGISFDKFGRTSTPQQTEI
                     VQGLFKDIDAAGFVKKQAIEQLYCESCGRFLADRYVRGTCPHCAYPDARGDQCESCGK
                     LLDPTELKEPKCSTCGAAPKPRSTSHLYIDLPAIRPRLEDWMKEASVKGFWANNAVQM
                     TQAWIRDGLRERAITRDLKWGIPVPKPGFEKKVFYVWFDAPIGYISITACHTDENGGD
                     WKSWWQSPGNVELYQFIGKDNIPFHTVIFPSTLLGSGRPWTMLHHMSSTEYLNYESGK
                     FSKSKGIGVFGNDAVDSGIPADVWRFYIYYNRPEKSDVTFTWSDFQEKVNGELIGNLG
                     NLANRTLTFVQRFYDGRVPEGEPDPAFWAEVRKVYQKITEHLERAELRDAFRAAFSIS
                     GIANKRFQDGEPWKARTQDPAKAAGLIRDLCYVLKDLAILMHPYIPRAADRLAGFFGL
                     KVGGADGLSWKDLGVLEGIGRVNSPEVLFSKLEDDRIAELRERYAGSQKDRADREAAK
                     TSAEGPAARTPTASPAAAGPESVSPAAAAAPAPAPAPAPAPYADLPVEERFSKLVDLR
                     AARIVKIERHPKADKLYIETLDDGSGTERVIVSGLVPFYKEEELLGKTIVLVNNLKPA
                     KLRGVESRGMLLAASKDGPEGREVVEVLDVPWAAPGARVFLETGAGEPPAEIDIDTFF
                     SIPIRSEGGTVKVGTKTL"
BASE COUNT         1524 a         3098 c         3076 g         1491 t
ORIGIN      
        1 cctgcggaca ggacccgggt atattccgta ccgatcccgg ttcccgctcc atgagcaccg
       61 agcggacctt cggccgggac atcgccgacc cggaaaccct ggagtccctg cttctgggga
      121 tggcccagga gatctcccag agggcctggc gtgagggtgt ccggtcgaaa acggtcttca
      181 tcaagctgcg gtacgcggac ttccgaaccc tgaatccccg gaggacgtcc tcctccttct
      241 tcgaggaatc cggggtcctc tggtccgagg cgtgcaggct gttccgatcc gcctggaacg
      301 gcggacccgt gcggctcctg ggcctggggc tcgggaacct ccaggaccgg aagccggaat
      361 cccaggggga gctcttcccg gccggggacg accgccgggc gcgggtggaa caggcggtct
      421 tcgaactgga gcgcaaggga gtcggaaaag tcacccgggc ccgcctgatc gggagggagg
      481 gggatcggat cccgggcgat ccctgatccg gacaaagtgt cccgacggga tcccgaaaac
      541 cgctcagccc tcggtcactg ttccacggct ttcttgatct ccgggacgcg acggttcaat
      601 tccgcggtca gggcgtcccg ggccttgagg aaggcgtccg agaccgaggc cgaagccgtc
      661 cctccctggc cgcggacggg atccaggggc agggcgaaga ctatggtgcc gtcgtcccgg
      721 atgatccgga tcgtggtctt gcagtcgctg gtgaagatgt tgtacttctt cccgccgtac
      781 tccatctgcc gggccggcga ggcttccgag accacgaagg cgaagtagga ggccccggcc
      841 gaggcggcga gggcccggac cgaggcgggg tcgcccccga aggcccggtc gaacccggat
      901 ccgagcagct tgccgtccag gggagaggga accaggccgg cccccttcaa tatcgcggcc
      961 acggagtcca ggacctgagg gttctgaacc gccccctccg gtccccgttc cagggccact
     1021 accttggcga cgttcgaggg gggcaggtag gagaagtccc gctgcacgtt ccggaacacg
     1081 taggtgaagc cccggaccgt gaaggccgga taggcgcgca ccaccgcctc cgaggaaggg
     1141 ctgtccaggc gggagaggat cgcgttcgcg cgtccgtaga tgtccgtgga gacgctggtc
     1201 gtcagtacgc cccctccccg gacgaactcg aaccggatgg gggcgtccgc gaccggggac
     1261 ttggcggcgg cgaagttctc gaacaggaag accgcgggct ggagggatcc ctcctttccg
     1321 acggaacggg aggccgcggc cgcctgggaa ccgtcggggc cgatccatcc ttccccgggc
     1381 tccagggaca gccggtttcc gatgcgcacc agttccgcct ccgcctgcga acccaggcgg
     1441 gcggcctccg ccgagccgtc ctcccgggag agggccagga cggtcacgta ggatcggatg
     1501 gcctctgcgg gatggttgga gtcctgggcg gtccgggcga cctccaaaag ccgcttcatc
     1561 tcgtccacgg ccgaagccgg tcggaccgtg ccggatccgg gcttgggacc cgggccgggc
     1621 tcgagggcgg cgggggtcga gacacagccc gcgcaaacga ggatcgaaca tgccgctacg
     1681 acgacgagag ctccgatctt catggggact cctcccggga gatataaccc ggacgcgcgg
     1741 ccggccgcac gatcgtttcc gtatgaggga ctcactcgaa cacctcgctg tggatctgga
     1801 cgtcccgcac cctgcactga ccctccgcga aggccaggac cttctggagc acgagctccc
     1861 cgtgctcccg ggaattggag accagggcgc cgccgagctg ggcccactgg agcgagtcct
     1921 ggaggtcgac ttccgcgcag gagatccgga acttgaccct cagcttttcc ttcaaggaaa
     1981 ccacgatctt ccgtttctcc ttgatgctcg tggtttccgg gagttccagg atgaactgga
     2041 tcatggacac taccacgagg acctcttgcc caggagcccg gccagctcgt cctgaccgac
     2101 cacgggatcc gcgattccgt tttccgggtc gcccggggag cggaaagcgc ggagcccctt
     2161 agccccgagg gcggcggccc cgaggaggag cagggcttcc gggagcagta ggagccggag
     2221 cgacgcggag aaggagatcg aatcgggcag gagggcttgc gcgaaggccc ggaagagctc
     2281 gaagaacgcc aggatgaaca ggatggccgg tcgggattcc ggcaagatcc tgcgcagccg
     2341 gagtatccgg aggctacgga cggccaggag aaccaagggg atcgcatccg gccgcccttc
     2401 caggtgggcg taggcaaggg gtgccgaggc gaaaacgatg accgggaggg aggatatcac
     2461 ctcaatccag gaatcccgcg gggaccttcc ggattccagg gccgcgaggg cggtcagggc
     2521 gcttccgaag cccaggatgc aggcgtccag ggcgagcccc gccaaggaag cccacttcga
     2581 ataggggccg agagccggcg cgaggacgga gatctcctcg aacgcccagg ccgccaggga
     2641 cacggcggcc gagaccgcga gcaggatgcc cagcggaccc ggggatcttt ctttcatcgt
     2701 cggccttgtg atgcggttca tcgccgcgct actatcatac aagcctatca tgggaaagaa
     2761 aagcgccgtc gccgcgattt ttttgttggc cgccctgctc gcggcggccc agtccaccga
     2821 ggggacggaa gccgaacctg cgccgcagga ggtgcccgcc tccggatccg cccccacgcc
     2881 tccgcccctg gcgccggtac cggtcctccc ggtcgaggcg ccccagagcc tactttccct
     2941 ccggatgggg gacaaggacg tggaactgct ggccgaggga tactgggagg tgtcggtctt
     3001 ggcttccggc gccttcggtc tgggggccaa cgagggagtt tccgccccga ccctcctctt
     3061 tcggcagatc ccggacctat ggctctccct gaccctcctg gaccgatggt tcgtggaagc
     3121 cagcctgtcc tccgagggct tcggggacca gtacgtggcg ggataccggg gaggggaggg
     3181 ggacttcctg cgggaggtcc ggttgggcaa caagggagtc tcgtttccgg accttcccta
     3241 cctttccctg ggagccggaa gctacacctc cttcggaatt tctgccctcg gccgcagcga
     3301 ccgggggtcc gcccatttcc tggtgcggta cgatcaggcc cgaaggatca cccagacctg
     3361 ggtcgggagc cgggaagtca cccgcacgga gtaccggccc tcggaatacg tccgggggcg
     3421 gtggttcatg ctgccggacc agggcgtgtc ggatgtcgag ctctatatcg aaagcagttc
     3481 gggaacgatc ataggctcgg atactcgaaa atatcgaaca atgactgcgg atgagtattc
     3541 gctttccgcc gcgacgggcc gcgtggacct gaagaccgcg gtgggggagg ggcggcggct
     3601 cctcgtctac taccccggtt tgaatgtttt ggcgattacc tatgacgacg gccctccttt
     3661 ctacaatgaa cctccggccg gagtaaccgg agtggcatcc ggtactgccc tcgtcctcta
     3721 cgagcggggc gaacacgagg gcttccagat cgccaaccgc tacgccctgg cctcctcggc
     3781 ctccgcggcg tcagcgatcg cgtacgtcag ggacgcggcc acgggactgc cggatccggc
     3841 gtacacggcc acggttcttt ccgacggatt cgcacgggtc acccagaccg ttgcggtcac
     3901 atcggctccg gatttttccg actacgattt ccgcatgccc ttcgcgggcc cgccccttac
     3961 cacgatcgac gtaggcatgg actggctgta cgatcccgat gccgtggagg ccgcggaggg
     4021 cacgcctacg gagcccgccc cggcgtactc ccgggtcctg gtggtggaga cctacggcgc
     4081 cccgggaagc ctggtcctgg aggagacggg ggtcctgccc ggatccgtgg aggtgcgccg
     4141 caacggggtc ctggactacg ggttccggta cgacccggcg tccaacgtcg tgactctgga
     4201 ccgggatccg gcgatctcgg acatcatcga ggtttcctac ctcctggcct cctcggaccg
     4261 gaacgccggg agcctggccg cgggcctggc cggaatcttc gacctttcac cggactggag
     4321 ggcctggacg gccctgggac tccgctgggg cgtgcccgga acggggtatt ccgagggagg
     4381 ggacgcggtc cccgggtccc tgaccctgac cgcggggata cggggcggat cgccggaacc
     4441 cgatgccggg ccggacgctg ccggcgcgcc gcggttcaag gcggaggccg ccctggcagc
     4501 gagctaccgc cgccccgacg cctcggggta ctatcggctc gcgggcatgg aggaggcatc
     4561 ctcctggacc agccccttcc ggcctacggg gacctggccg gcgggagttg gcgtcgcgac
     4621 ggtggcggcc gtggcggata ccgcggcccc gagcctggag tccctgttcc cgaaactgta
     4681 cggccgcttc cattcggcga cttccattca gaaggtccta caagtctcct tcgccgggca
     4741 tgacggaacc acggccgagg tagtccggta cgaggaaccc gtcgcagtcg gggactatcg
     4801 ggccttcgcg ttcttcgctc ggcgggattt tgatgccgcg ggagccgtgt tgacggtcgc
     4861 cctggacggc gccgaccctg ccatctccgt ccaggtgccc gccggtgaaa tggtcgaaac
     4921 ctggcgccgc tttgtcgtac ggtacgacgg agtcgccaaa ctctactacc aggataccga
     4981 ggacggcccg gagcacgaga ttggcgtgat tcccgatccg gacccctccg tcagcgcagg
     5041 tcggatcacg atctccgtga ccggtgtcac ggacggctcc gtcttcgtcg acgagttcca
     5101 cttcctggat cccgcggggg agatcgccct gtccggtcga ggctccttct cctggagccg
     5161 gggcggaacc ttggcggaat ccggggcagg cgcgatcctt tcggatccgg gcttttcggc
     5221 ccggctctcc ggcacgatct ccgaggactc gaacttcgcg ggatacctgg acggcaggat
     5281 cgcggtgggc ccggccaagc tggcggcgaa cctgcgacag gggtacgcgg acggggagct
     5341 ctcggacgga gccttcggac actccctgga actgccctta aaagggtttt ccgtaggaga
     5401 cagtttccag ttcgattccg gctcgggccg tttcggccgc agggattttc tgtccctgca
     5461 ggcccggggg ctcggactcg acctggagca gtccgcgtcc tacgggggat cggtcctgga
     5521 gcaggcctgg aaggtgcggc tcgccgcggg atcctggatg accctgggcg gcgagctctc
     5581 caaccgggcg gccggcgcgg tcgacctctc ctccgcctac ggggaagcct ggatcgaggc
     5641 cttccggtac gccctgccgg ccctggagga cgcctccctc cggcgcacca cgaccctgac
     5701 gggcgcctgg aagctgggcg gcggcgccct gacggtggcc gcggactccg ggctcacgga
     5761 cgcggtatcc ccggcgtccg ccggttccgc cggcttgaac gcccggctgg ggtggtccat
     5821 cccggcgggc cggggctccg tcgaacccta ctaccgccgc gtctggaccg ccggcgccgt
     5881 ctcgcagtcc tcggccttcc cggaggattg ggaattctgg atcgaccggt tctcctcctc
     5941 cgagtatctc tacaggacca tccccttcgc ggagcttttc gatcccttca cagccggtct
     6001 cttcgaggcc gcggcccagg ggacgacctc gagctcctat aaacccgaga cggggatcct
     6061 gttcggacga cgctacggct cgagctggac ggacctggtg atcccgagca aggcggccct
     6121 gtccctgcgc agggagctgg cttcctccct ggagactgtt acggacgccc tggtgtggga
     6181 ggcgtccgcg tccatggccg cggtcaacct cttcggttcc cggggaacct accggttttc
     6241 ccggctctac tcggtggacg agtactccca gcgtttcgca ttctcgctta cgtcctaccg
     6301 cggggatccc gatcccctgg cctccttttc ccataccgcc ctggcctcct tctacacgac
     6361 ccgggaggac aacctggtgg cggagaaccg cctggacctg acggaatccc gggacggtct
     6421 ggcctggagc gagcggctgt ccctgaacct gacactccgt ccttcccgga cctggctggg
     6481 ggacctggtg accctgctcc tggaaaggtc cggctccaag cccgaaccgg aggaagggac
     6541 cgggccgacc ctggtttccg gattctggga ttccctccgg accgcccgcg gccggctttc
     6601 cgaggtctgg ggggcctcct tcctggtcaa gcgaccctac gcctaccagg accggttcga
     6661 cgtagagctt gcccaatcct acgagaccaa gtacgtggtc tcccagaaag ccagcctctg
     6721 ggcccgcctg ggcacccggc agaccctgaa catcctggag ggagccgatt actggatgct
     6781 ggggttcgag ctgaccctgg gcgcccgggt catcttctga cagccctcgc tcgggggacc
     6841 cggacaggtt cggcaatccg cggactcccg gaaccttgac atgccccgcg ccccgggacc
     6901 atattgaacc gccatgaaaa gaagactcat caccagtgca cttccgtacg tgaacaacat
     6961 cccccacctg gggaacctaa tccaggtcct ttccgcggac gtgttcgccc gcttttgccg
     7021 cctccggggc tacgagaccc tctacgtctg cggcaccgac gagtacggta ccgccacgga
     7081 gaccaaggcc ctggaggagg ggatcacccc ccgggaactc tgcgaccgct tcttcgtggt
     7141 ccaccgggac atctacgcct ggttcggcat ctccttcgac aagttcggcc gcacgagcac
     7201 cccccagcag accgagatcg tccagggcct gttcaaggac atcgacgcgg cgggattcgt
     7261 gaagaagcag gcgatcgagc agctctactg cgagtcctgc ggacgcttcc tggcggaccg
     7321 ctacgtccgg ggcacctgcc cgcactgcgc ctacccggac gcccggggcg accagtgcga
     7381 gtcctgcggc aagctcctgg atcccacgga gctcaaggag cccaagtgct ccacctgcgg
     7441 tgccgccccg aaaccccggt ccacctccca cctgtacatc gacctgccgg ccatccgacc
     7501 caggctggag gactggatga aggaagccag cgtcaagggc ttctgggcca acaacgccgt
     7561 ccagatgacc caggcctgga tacgggacgg gctccgggaa cgcgccatca cccgggatct
     7621 gaagtggggt atcccggtcc ccaagccggg cttcgagaag aaggtcttct acgtctggtt
     7681 cgacgcgccc atcgggtaca tctccatcac cgcctgccac accgacgaaa acggcgggga
     7741 ttggaagtcc tggtggcagt ccccggggaa tgtcgagctc taccagttca tcggcaagga
     7801 caacatcccc ttccacacgg tcatcttccc ctccaccctg ctggggtccg gccggccctg
     7861 gaccatgctc caccacatgt ccagcaccga gtacctgaac tacgagagcg ggaagttctc
     7921 caagtccaag gggatcggtg tcttcggcaa tgacgccgtg gactccggga ttcccgcgga
     7981 cgtgtggcgc ttctacatct actataaccg ccccgagaag agcgacgtga ccttcacctg
     8041 gagcgacttc caggaaaagg tgaacgggga gctgatcggc aacctgggca acctggccaa
     8101 ccgcaccctg accttcgtgc agaggttcta cgacggccgg gtccccgagg gagagccgga
     8161 cccggcattc tgggccgagg tgcgcaaggt ctatcaaaag attaccgagc acctggaacg
     8221 ggccgaactg cgggacgcct tccgggcggc cttctcgatt tccggaatcg ccaacaagcg
     8281 cttccaggac ggcgagccct ggaaggcccg gacccaagat ccggccaagg ccgccggcct
     8341 catccgggat ctctgctacg tgctcaagga cctggccatc ctcatgcatc cctacatccc
     8401 ccgggcggcg gacaggctgg ccggattctt cggcctcaag gtcggcggcg cggacggact
     8461 atcctggaag gacctcggcg tcctggaagg gatcggacgg gtgaactcgc cggaggtcct
     8521 tttttccaag ctcgaggacg accgcatcgc ggagctccgg gaacggtacg cagggagcca
     8581 gaaggaccgg gccgaccggg aagccgcgaa gacctccgcc gagggcccgg ctgcccgaac
     8641 cccgacggca tcgccggcgg ctgcgggtcc cgaatccgtt tccccggccg ccgcggcggc
     8701 gcccgcgccg gcgcccgcgc cggcccccgc accctacgcg gatctccccg tggaggagcg
     8761 gttttcgaag ctcgtggatc tgcgggccgc gcggatagtg aagatcgagc gccatcccaa
     8821 ggcggacaag ctctacatag agaccctgga cgacgggtcc gggacggagc gcgtcatcgt
     8881 ctcggggctg gttcccttct acaaggagga ggaactcctc gggaagacca tcgtgctcgt
     8941 gaacaacctg aagcccgcca agctccgggg tgtggagagc cggggcatgc tcctggcggc
     9001 gtccaaggac ggtcccgagg gacgtgaggt cgtggaagtc ctggacgttc cctgggccgc
     9061 gccgggcgcc cgggtgttcc tggaaaccgg agccggagag ccccccgccg agatcgacat
     9121 cgacaccttc ttctccatcc cgatccgttc cgagggaggg acggtaaaag tcggaacgaa
     9181 gactctcca
//
LOCUS       DAOTEI010000146         9141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5382_length_9141_cov_5.323465, whole genome shotgun sequence.
ACCESSION   DAOTEI010000146 DAOTEI010000000
VERSION     DAOTEI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5382_length_9141_cov_5.323465"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..908
                     /locus_tag="P5313_12735"
     CDS             102..908
                     /locus_tag="P5313_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81279.1"
                     /translation="MILVRVRYSVFLLAISLGATVGAGVWSYSVAAAAGMKGPAETSL
                     FLALCAAAVQILAAAELFRQARRKRRELDSIAGAVRYGGILPAERLDRFGPLGDSIRR
                     ILKDLSDAGERKSERIASLTGLLRASLALVETPVLVVSPDGLVQEASKGAREDSRLSG
                     LETGKTRVEDILPEAQMRAVLREADRTHQAVELPGHLTFHPVYSTRGQIACFLADLGR
                     TGIRDFLGGLGERKQAPEPAAAKPPNILGRVLGRFGRGSKDTSDRRRGAD"
     gene            968..3439
                     /locus_tag="P5313_12740"
     CDS             968..3439
                     /locus_tag="P5313_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY81280.1"
                     /translation="MCGIVSLCYEDEEAGMGIEAAALLRRLEYRGYDSTGASFIDAAR
                     KIVLVKSVGAPSRVCRDLGIDKFPGQRFIGQVRWATYGAVTDANSQPHHVRCKVEMVG
                     AHNGNISNTDSLKAELTAQGHRVVSENDGEIVVHLVEDAWAENRRNPGPALAAQKRAL
                     SACSLSDVPDEVLLMVDAVRRAESRTEGSYAAAVSDPTVPGVFAVKSGSSLYAGIGAD
                     ERGSFVVVSSDLTSVLSKTRTLIPLAEGEGLWFTHDRYLVFSLAGEPVYSRPRPKRSK
                     LNVQDTALDPRYSYYMEQEISSSPSNIEEILRYYFRDPGAAALEAGLEDKRLIAKESL
                     DRLLALTEELDEDRLRADAESVFSDPAWRELAARVKDYAPRPESAYRGDERAFLAELA
                     KLLPGRLSDLALADSALVWRKRRAVVRRVGELSQAVRETVKAGGRVYLVASGTSYHAA
                     LTAAYFFDHLAGLPVYPCNPGMFRSLYLDTLEDRDLILGITQSGETKDLVDILQDARD
                     KAPGLRRVSLVNNENSRIPQELSDFYLPLLCGPEIAVAATKSFLNQIVILYLLAAGFR
                     MPEVEVRKKVTRIRGLLEDTLRLTLPEADRAAERLFREPSMHILGTGLIGLAREGALK
                     IREVVLNHSEGYDAAEFKHGPNTILGKNTVFGLQSLEAVLSAYAGVLRDAPEAERKAL
                     LAAAPSAVLAKNPGILEAGFGNYPLVFVCPPDERDIRITISQIHTHKIRGADILLVAE
                     KRPELALAVEGKPANDPNYRSLYLEIPSTGDRDLFVFSATLVLQWLAFRMSVRKGAYL
                     DGLGVQDHGVHPDVPKNVSKSITVD"
     gene            complement(3653..5569)
                     /locus_tag="P5313_12745"
     CDS             complement(3653..5569)
                     /locus_tag="P5313_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HRY81281.1"
                     /translation="MFVVGTAGHVDHGKTALIRALTGIDPDRLPEEKARGMTTDLGFA
                     YFEGPGGECVGVVDVPGHERFLRNMAAGAWGLDLALLVVAADDGWMAQSRTHARILAS
                     LSAPDVLLAINKIDAVGPERAEEVARDAAEKAAALLGFRPDARFVSARTGEGVPELKG
                     AILERLFPDRRRLSGKPYLFVDRVFTLSGAGTVAAGTLRGGPLSVKDELVLWPAGEKV
                     RVRSLQTYKAGTELALPVSRTALALPKPRSELRRGDLLAGSGMEVLSGREFLCLLEPL
                     PGDTELCPRDEKGRLLLRPGLEAELVLGSARRDAVVSPYRTPGWLRVVSSEPLACPPG
                     QPFALLRRGGTELLARGRVMAEGATDIRARRTLDAVLPGVPALAAGLGSARGSAAGGE
                     PGADAGRRIPGEDDGAVPVRRALEILSRGWTLFEPGADRPSDPAAPQNLRVLTGQEGR
                     FFFSAESFDRFRVRLLETAGKPGGAVRAELESASGLDTGACSALLGALEAEKALERSG
                     PRWNVPGPGPKLGKEEARLEALLIRADAEGVEPGRNVPQQDGRTLKALCALGRALALD
                     TGVFWHLSVYERLSAAVLAGRAAGERFSVGQAKDRTGLSRKYILPLLNRMEDEDLVKR
                     EGDVRVVLPKGARD"
     gene            complement(5570..6967)
                     /gene="selA"
                     /locus_tag="P5313_12750"
     CDS             complement(5570..6967)
                     /gene="selA"
                     /locus_tag="P5313_12750"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011898649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRY81282.1"
                     /translation="MKTNERFSRLPQVERLLARPEVAGYFGQISRPLCARAASRAVAV
                     LRERLASDPAYPSDEETLLDGALELLRAELDALDRRRIRPVLNGTGILLNTNLGRSPV
                     PGEDWDAVRETNTGYSNLEYDLAEGRRGGRGGLVPDLAAELCGAEAALAVNNNAAGVL
                     LMLTALAKGREVIVSRGEQVQIGGGFRVPDILALSGARLVEVGTTNVTTREDYLRAVG
                     SDTALVLLVHASNFALRGFSSSPGIRELADALPEGVILAADQGSGCTGDSMAGELPAR
                     KYLERGCRLVAFSADKLLGGPQAGIVAGDRDLIEALGRHPLYRAFRPGKTILTLLERR
                     LAARLNGEPSRAEEARDRDPKELRRYGRRILSRLPRGRARLVPSRAAWGGGSTPDETF
                     PSLALELGPAGPPERLLTALREAPVPVVGLIRDDRVLLDLSTLWGTEPALAAASVSDA
                     LARDELAAQETGRVR"
     gene            complement(6971..7060)
                     /locus_tag="P5313_12755"
     tRNA            complement(6971..7060)
                     /locus_tag="P5313_12755"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7024..7026),aa:Sec,seq:tca)
     gene            complement(7387..8253)
                     /locus_tag="P5313_12760"
     CDS             complement(7387..8253)
                     /locus_tag="P5313_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY81283.1"
                     /translation="MKKAVLILLVLALTVPAFARGIDAVVSADWLAANLSKVVVVDMR
                     KLDEYRAGHIPGAVSLQGLYVAKDNLSNEVPAADELAELIAEAGISADSKVVVVETDG
                     SRFAWVTRAAWTLVYAGVADVAVLDGGYAAWTKAGKPTATGMEFRDETKFEVKFNPAY
                     IATKAEVLAGLKKAQILDTRAYDTYFGLSKQAFVDQFGHIPGAYSLPSAWITKDGMVR
                     PKAELEEMVAALKLSPKKPMIIHCDSGVLCTAWWWILTQQMGWTNVKSYDGSAQEISK
                     EPTVKFVKYVWK"
     gene            complement(8333..>9141)
                     /locus_tag="P5313_12765"
     CDS             complement(8333..>9141)
                     /locus_tag="P5313_12765"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY81284.1"
                     /translation="AAGMKHGVSGRFSEAEKKTFDAKVFEQSCRSCHASCGDCHVKSP
                     VVSGVNLGLMNGHQFARKDESKTCALCHGGRVYPEFTGEYGGTPDVHYRKGMTCADCH
                     PAEQFHGDGKAYPDRTKVAVKPSCVTCHPLEYLGKDSTKQTHETHDGRVSCSACHTAS
                     SYRQCSSCHLGEGARSSPAIVLGKNPRNLKELTTLRLIPTVRDTFQKAGIAQANFDAL
                     PNYWNTVPHNIAKRTDRTRDCNTCHQEGLYYLEAKMLPPGSSKKNLDLIP"
BASE COUNT         1415 a         3132 c         3185 g         1409 t
ORIGIN      
        1 tgttcccgga acccggggac gcattcgagg agggggaccg cctcttcgtc ctgggcagga
       61 actcggacct ggacgagttc cggaaaatct agggggaccg catgatcctc gtacgggtcc
      121 ggtattcggt cttcctgctc gccatatctc tgggggccac ggtcggggcg ggggtctggt
      181 cctattcggt cgccgccgcc gcgggcatga agggacccgc ggaaacctcg ctcttcctgg
      241 ccctctgcgc agcggcggtc cagatcctgg ccgccgcgga gctgttccgc caggcccgca
      301 ggaaacgccg ggagctggac tccatcgcgg gcgcggtacg gtacggaggg atccttcccg
      361 cggagcgcct ggaccgattc ggtcccttgg gggacagcat acgcaggatc ctcaaggacc
      421 tgtccgacgc gggggagcgc aagagcgaac ggatcgcctc cctgacgggc ctcctgcggg
      481 cgtccctggc cctggtggaa acccccgtcc tggtggtatc gccggacggg ctggtgcagg
      541 aagccagcaa gggagcccga gaggattccc ggctttcggg cctggaaacg ggaaagaccc
      601 gggtggagga tatcttgccg gaggcacaga tgagggcggt gctccgggag gcggaccgca
      661 cccaccaggc ggtggaactt ccgggacacc tgaccttcca tccggtatac tccacccgcg
      721 ggcagatcgc ctgcttcctt gccgatttgg gacgtacggg gatccgggac ttcctgggcg
      781 gcctggggga gcggaaacag gctcccgagc ccgcggccgc caaaccgccc aatatactcg
      841 gccgggtact gggcaggttc ggcaggggaa gcaaggatac gtcggaccgg cggcggggcg
      901 ccgattgact cgatgcccgc cggcccggac ttcggattcg atacaaccaa taggagaggt
      961 ggacaccatg tgcggaatcg tttccctgtg ctacgaggac gaggaggccg gaatgggcat
     1021 cgaggcggct gccctgctca ggcgcctgga gtaccgcggt tacgactcca ccggggcctc
     1081 cttcatcgac gcggcccgta aaatcgtcct cgtgaagagc gtcggcgctc cctcccgggt
     1141 ctgccgggac ctggggatcg acaagttccc gggacagcgc ttcatcggcc aggtccgctg
     1201 ggccacctac ggggccgtga cggacgccaa cagccagccc caccacgttc gctgcaaggt
     1261 ggagatggtc ggggcccaca acggcaacat ctccaacacc gacagcctca aggccgagct
     1321 gaccgcacag gggcaccggg tcgtgtcgga gaacgacggg gagatcgtgg tccacctggt
     1381 ggaggacgcc tgggcggaga accgccgcaa ccccgggccc gccctggcgg cccagaagcg
     1441 ggcactttcg gcctgcagcc tttccgacgt ccccgacgag gtcctcctca tggtggacgc
     1501 ggtccgccgg gccgagtccc ggacggaagg gtcctatgcg gcggccgtct cggacccgac
     1561 ggttccgggg gttttcgccg tcaagtccgg gtcctccctg tacgccggca tcggagccga
     1621 cgaacgggga agcttcgtcg tggtgtccag cgacctcacc agcgtccttt ccaagacccg
     1681 gaccctgatc cccctggccg agggggaggg cctgtggttt acccacgacc ggtacctggt
     1741 gttctccctg gccggggagc ccgtctactc ccggccacgg cccaagcggt ccaagctgaa
     1801 cgtccaggac acggccctgg accccaggta ttcctactac atggagcagg agatctcctc
     1861 gagcccctcc aacatcgagg agatcctgcg gtactacttc cgggatcccg gggccgcggc
     1921 cctggaggcg ggcctggagg acaagcgcct gatcgccaag gagagcctgg accgcctcct
     1981 ggccttgacg gaggagctgg acgaggacag gctgcgcgcg gacgccgagt cggtcttctc
     2041 cgacccggcc tggcgggagc tggccgcccg ggtcaaggac tacgcccccc ggcccgaaag
     2101 cgcgtacagg ggcgacgagc gtgccttcct cgcggagctg gcgaagctcc ttccgggccg
     2161 cctatcggac ctggccctgg cggactcggc cctggtctgg cgcaagcggc gggccgtggt
     2221 ccgccgggtc ggggagcttt cccaggcggt ccgggagacc gtgaaggcgg gcggacgggt
     2281 ctacctggtc gcctcgggca cctcctacca cgccgccctg accgcggctt acttcttcga
     2341 ccacctggcg gggcttcccg tctacccgtg caatcccggc atgttccggt ccctctacct
     2401 ggacaccctg gaggaccggg acctgatcct ggggatcacc cagtccgggg agaccaagga
     2461 cctggtggac atcctccagg acgcccggga caaggccccg gggctccgca gggtgagcct
     2521 ggtgaacaac gagaacagcc gcatccccca ggagctctcg gacttctacc tgcccctgct
     2581 ctgcggaccc gagatcgcgg tggccgccac caagagcttc ctgaaccaga tcgtcatcct
     2641 ctacctcctg gccgcgggct tccgcatgcc cgaggtcgaa gtccgcaaga aagtcacccg
     2701 catccggggc ctcctggagg acaccctgcg gctgaccctc ccggaggcgg acagggcagc
     2761 ggagcgcctc ttccgggagc cctcgatgca catcctgggc acgggactca tcggcctggc
     2821 ccgggagggg gccctcaaga tccgggaagt ggtcctgaac cacagcgaag gctacgacgc
     2881 ggcggagttc aagcacggcc ccaacaccat tttgggcaag aacaccgtct tcggccttca
     2941 gtccctggag gcggtgctct cggcctatgc cggggtgctc cgggacgcgc cggaggccga
     3001 acgtaaggcc ctgctggccg cggccccctc ggcggtcctg gcgaagaatc ccgggatcct
     3061 ggaggccggg ttcggcaact accccctggt cttcgtctgc cccccggacg agcgggacat
     3121 ccgcatcacc atcagccaga tccacaccca caagatccgc ggcgcggaca tcctcctggt
     3181 ggcggagaag cggcccgagc tggccctggc ggtggagggc aaacccgcga acgacccgaa
     3241 ctaccggtcc ctgtacctcg agatcccctc caccggggac cgggacctct tcgtcttctc
     3301 cgcgaccctg gtcctgcagt ggctggcctt ccggatgtcc gtccggaagg gggcctacct
     3361 ggacggactg ggggtccagg accacggggt ccacccggac gtgcccaaga acgtgtccaa
     3421 gtcgatcacg gtcgattagc gcaggttctc tcagttttct ggacttaacc acggagagca
     3481 cggagagcac ggagaagaga gaagtcgagg aacgagtgtc ctgctgaggg gcggcggtca
     3541 tggttatccc atagtcgccg ccgtcgttcc ctatcttttc atcactctct taatttttta
     3601 tcttgtaaat ctttcttaat cacgttcgtc ttgttggaac tatccgggaa ccctaatccc
     3661 gcgcgccttt cggtaaaacc acccggacgt ccccctcccg cttcacgagg tcctcgtcct
     3721 ccatgcggtt gaggaggggc aggatgtact tccgggagag ccccgtgcgg tccttggcct
     3781 ggcccacgga aaaccgttct cccgcggcgc ggcccgcgag gacggccgcc gagaggcgtt
     3841 cgtagacgga gaggtgccag aagaccccgg tatccagggc gagcgcccgg cccagggcgc
     3901 agagggcctt gagggtccgc ccgtcctgtt gaggaacgtt ccggcccggc tctacgccct
     3961 cggcatcggc ccggatcagg agggcctcca ggcgggcttc ctccttgccg agcttcggtc
     4021 cgggccccgg cacgttccac cggggccccg agcgctccag ggccttctcg gcctccaggg
     4081 cgcccagaag ggccgagcag gccccggtgt ccaggcccga ggcgctctcc agttccgcac
     4141 ggacggctcc cccgggcttg ccggccgtct ccaggaggcg gacccggaag cggtcgaagg
     4201 actccgccga aaagaagaac cgaccctcct gccccgtaag gacgcggaga ttctgcggcg
     4261 ctgccgggtc tgacggccgg tccgcccccg gctcgaacag ggtccagccc cgggacagga
     4321 tctccaaggc ccggcggacc gggacggccc cgtcatcctc cccgggaatc ctccgtcccg
     4381 cgtcggcgcc gggctctccg ccggccgcgg aacctcgggc tgatccgagc ccggccgcca
     4441 gggccggaac cccgggcagg acggcgtcca gggtccggcg cgcccggatg tccgtggctc
     4501 cctcggccat tacccggccc cgggccagga gctccgtccc tccccggcgg agcagggcga
     4561 agggctgtcc gggggggcag gccaggggct cggaggatac gacccgaagc cagcccgggg
     4621 tccggtaggg ggagacgacc gcgtcccgcc gggcgctgcc caggaccagt tccgcttcca
     4681 ggcccggccg caggaggagc cggcccttct cgtcccgggg acagagctcg gtatccccgg
     4741 gcaggggctc aaggaggcag aggaactccc ggcccgaaag gacctccatc ccggagccgg
     4801 ccaggaggtc cccccggcgg agctcgctcc ggggcttggg cagggccaaa gccgtccggg
     4861 acacggggag ggcgagctcc gtgccggcct tgtaggtctg gagggagcgc acgcggacct
     4921 tctcgcccgc gggccagagg accagctcgt ccttgaccga gagggggccg ccccgcaggg
     4981 tgcccgcggc cacggtcccc gcgccgctca gcgtgaaaac ccggtcgacg aagaggtagg
     5041 gcttcccgga aaggcgcctc cggtccggga aaagacgttc aaggatcgcg cctttcagct
     5101 ccgggacccc ctcccccgtc cgggcggaga cgaaccgggc gtcggggcgg aagcccagaa
     5161 gggccgcggc cttttcggcg gcgtcccggg cgacctcctc ggcccgctcc gggccgacgg
     5221 cgtcgatctt gttgatcgcc agcaggacgt ccggagcgga caggctggcc aggatgcggg
     5281 cgtgggtccg gctctgggcc atccaaccat cgtccgctgc caccaccagg agggccaggt
     5341 cgaggcccca ggccccggct gccatgttcc gcaggaagcg ctcgtggccg ggcacatcga
     5401 ctacccccac gcactcgccc ccggggccct cgaaataggc gaagcccagg tccgtggtca
     5461 tgccccgggc cttctcctcg ggcaggcggt ccggatcgat gcccgtcagg gcccggatca
     5521 gggccgtctt gccgtggtcg acgtgcccgg cggtgccgac gacgaacatc tagcggaccc
     5581 ggccggtttc ctgggcggcc agctcgtccc gggccagggc gtccgacacc gaggccgcgg
     5641 ccagagcggg ctccgtcccc cagagggtgg agaggtccag gaggacccgg tcgtcccgga
     5701 tcaggccgac gaccggaacc ggggcttccc gcagggcggt caggaggcgc tccggcgggc
     5761 ccgcggggcc cagttccagg gccagggagg ggaaggtctc gtccggggtg gagcccccgc
     5821 cccaggcggc ccgggagggg acgagccggg cccggccccg ggggagccgg gacaggatcc
     5881 ggcggccgta gcggcggagc tccttcggat cccggtcccg ggcctcctcg gcccgggagg
     5941 gttctccgtt gagccgggcg gccaggcgcc gttccaggag ggtaagtatc gtcttgcccg
     6001 gccggaaggc ccggtacagg gggtgccggc cgagggcttc gatcaggtcc cggtccccgg
     6061 ccacgatccc cgcctggggt cctcccagga gcttgtccgc ggaaaaggcg accaggcggc
     6121 agccccgctc caggtacttg cgggcgggaa gttctccggc catggaatcc ccggtgcagc
     6181 cggagccctg gtccgccgcc aggatcaccc cctcgggcag ggcatccgcg agctcccgga
     6241 tcccggggct ggaggagaag ccccggaggg cgaagttgga ggcgtgaacc aggaggacca
     6301 gggccgtgtc ggatccgacc gcccggaggt agtcctcccg ggtggtgacg ttcgtcgtgc
     6361 cgacctcgac caggcgggcc cccgagaggg ccaggatgtc ggggacccgg aagcccccgc
     6421 cgatctggac ctgctccccc cgggagacga tcacctcccg gcccttggcc agggcggtca
     6481 gcattaaaag gaccccggcg gcgttgttgt tcaccgccag ggcggcctcg gccccgcaga
     6541 gctccgcggc caggtccggt accaggccgc cccggccgcc ccggcggccc tcggccaggt
     6601 cgtactccag gttggagtac cccgtgttgg tctcccggac cgcgtcccag tcctcccccg
     6661 ggacggggct ccgtcccagg ttcgtgttga gcaagattcc cgtcccgttc aggacgggac
     6721 ggatcctcct gcggtccagg gcatccaatt cggcccggag cagctccagg gcaccgtcca
     6781 ggagggtttc ctcgtcggag gggtaggcgg ggtccgaggc caggcgctcc cggaggacgg
     6841 ccaccgcccg ggaggcggcc cgggcgcaca ggggccggga gatttggccg aagtaccccg
     6901 ccacctcggg ccgggccagg agccgctcca cctgggggag ccgggaaaaa cgttcgttgg
     6961 ttttcatgta cggaagaggc aggaatcgaa cctgccgccg cgcggaaacg cggccgacgg
     7021 ttttgaagac cgcgggagcc gccagacccc atcagcttcc ccggcgcccg gcgccgtccc
     7081 atcaatacta gagaccggga actgccgcgt caagttgacg gacgatccgc gcaaatctcg
     7141 acaggataac aggatctcta gcaggttatt gaaatacact aggaatttca ccaacctgct
     7201 aggatttgca ggatcaggag cggaaacagg ggacgcgggg cggacgccga cccgggtggt
     7261 gccaagcttt ttgtctcgaa gcttgtgatt cgtccggtcc ggcgtccgcg caaaaaacgc
     7321 ccggggccga aaccggcccc gggcgtcctt ttcttgtcct tccgttcact gtttaccgtt
     7381 aacagctcac ttccatacgt acttcacgaa cttgacggtg ggctccttgg agatctcctg
     7441 ggccgagccg tcgtagctct tcacgttggt ccagcccatc tgctgggtca ggatccacca
     7501 ccaggcggtg cacaggacgc cggaatcgca gtggatgatc atgggcttct tggggctgag
     7561 cttgagggcc gcgaccatct cctccagctc ggccttgggc cggaccatgc cgtccttggt
     7621 gatccaggcg gaaggcaggg agtaggcgcc ggggatgtgg ccgaactggt ccacgaaggc
     7681 ctgcttggaa aggccgaagt aggtgtcgta ggcgcgggta tccaggatct gggccttctt
     7741 gaggccggcc aggacctcgg ccttggtggc gatgtaggcg ggattgaact tgacctcgaa
     7801 cttggtctcg tcccggaact ccatgcccgt ggcggtcggc ttgccggcct tggtccaggc
     7861 ggcgtagccc ccgtccagga ccgcgacgtc ggcgacgccc gcgtagacca gggtccaggc
     7921 ggcccgggtg acccaggcga agcgggagcc gtcggtctcc accacgacga ccttggagtc
     7981 cgcggaaatg cccgcctcgg cgatcagctc ggcgagctcg tccgcggcgg ggacctcgtt
     8041 ggacaggttg tccttggcaa cgtagagccc ctggaggctg acggcgccgg ggatgtgacc
     8101 ggcccggtat tcgtccagct tgcgcatgtc gacgacgacg accttggaga ggttggccgc
     8161 caaccagtcc gcggacacta cggcgtcgat gccccgggcg aaggccggga cggtcagggc
     8221 caggacaagc aggatgagca cggctttctt catacgataa tctccttgcg atcgattgcg
     8281 ttcccgggtc cgccggcggc tcgaagccgc cggctcccgg aaccccctat cctcagggga
     8341 tgaggtccag gttcttcttg ctgctccccg ggggaagcat cttggcctcc aggtagtaca
     8401 agccttcctg gtggcaggtg ttgcagtccc gggtgcggtc cgtccgcttg gcgatgttgt
     8461 ggggcacggt gttccagtag ttgggcaggg cgtcgaagtt ggcctgggcg atgcccgcct
     8521 tctggaaggt gtcccgcacg gtggggatca agcggagggt cgtcagctcc ttcaggttcc
     8581 ggggattctt tcccagcacg atggccgggc tggaacgggc cccttccccg aggtggcagg
     8641 acgagcactg ccggtaggag ctggcggtgt ggcaggccga gcaggacacg cggccgtcgt
     8701 gagtctcgtg ggtctgcttg gtactgtcct tgcccaggta ctccagggga tggcaggtca
     8761 cgcaggaagg cttgacggcg actttagtcc tgtccggata ggccttcccg tccccgtgga
     8821 actgctccgc cgggtggcaa tccgcgcagg tcatgccctt gcggtagtgc acgtccgggg
     8881 ttccgccgta ctcgccggtg aactcgggat agacccggcc gccgtggcag agggcgcagg
     8941 tcttgctctc gtccttccgc gcgaactggt ggccgttcat gagaccgagg ttcacccccg
     9001 acacgacggg gctcttcacg tggcagtccc cgcaggaggc gtggcagctc cggcaggact
     9061 gctcgaagac cttggcgtcg aaggtcttct tctccgcctc cgagaaccgt cccgatacgc
     9121 cgtgcttcat ccccgccgcc g
//
LOCUS       DAOTEI010000147         9002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5492_length_9002_cov_4.823516, whole genome shotgun sequence.
ACCESSION   DAOTEI010000147 DAOTEI010000000
VERSION     DAOTEI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5492_length_9002_cov_4.823516"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="P5313_12770"
     CDS             <1..466
                     /locus_tag="P5313_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81285.1"
                     /translation="PMVFYEDRPVSWVLPTISLALSGIAWYAIWLRRFMVDEENKDYV
                     KFAVSKGLPWKRVMRGHVLRNALVPLVQYFPQQILLTIAGSLLIESIYSIPGMGGLLV
                     SAIRQQDNPLVQALVLLYSVLGVVGVFLGDLLMVLVDPRIKLAESSGKGAAA"
     gene            463..1395
                     /locus_tag="P5313_12775"
     CDS             463..1395
                     /locus_tag="P5313_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81286.1"
                     /translation="MIRTIPEVPAELFRPAAFDEAAAERTGYARYSYWRSTFRTFLKS
                     PVSIFLLALIVVLTTASFVYPLFAHMDLGAVNLDTETWNLSPNRDHVFGTDGLGRDIW
                     ALTWYGTRTSLLLGFVIALLDVGIGMIAGALWGYVRKLDRIMTELYNVLNNIPQTVFL
                     VLLAYIMRPGFWTIVTALCSTGWIGIARFVRNKVITIRDAEYNVASRCLGTPLRRMIT
                     KNVLPYLVSILIMEAALTIPYSIGSEVFLGFIGLGLPIDTISLGNLVNQGKNNFMLYP
                     YQLTWPTVILAAITVSFYIVGNRFSDASDPRNHV"
     gene            1392..2483
                     /locus_tag="P5313_12780"
     CDS             1392..2483
                     /locus_tag="P5313_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81287.1"
                     /translation="MSGDTILEVRDLRVSFSLRGRRLTALRGAGLDLRKGETLAVVGE
                     SGSGKSVLAKTFLGMLDRNGRVDSGSILFDGKDLARYNKEREWLGIRGKRIAMIFQDP
                     MTALNPLKTVGEQIREAAALHRGLDRVASRAVVLETLSRVGIPEPELRYRQYPHEFSG
                     GMRQRAVIAAAVACRPEILICDEPTTALDVTVQAQILDLIKSLQRELGMTVLFITHDL
                     GVVASVAQRVAVMYSGQVIEEGSVREVFYDPRAPYTWALLASLPQLGVKGEELYSIKG
                     TPPSLFKEVVGDAFAPRNPRALEVDFKHEPPVFPVSDTHWARTWLLDPRAPRIEAPET
                     VRRLRARLARGGAGAAATGGADTGKGGAE"
     gene            2480..3421
                     /locus_tag="P5313_12785"
     CDS             2480..3421
                     /locus_tag="P5313_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY81288.1"
                     /translation="MSRDVLLSVRDLRVDFPVDRKTFTAVRDVSFDVYRGETFSLVGE
                     SGSGKTTIGRAIMRIHPASSGEVLFEGRRVSGRIDAASERLLRQRIQMIFQDPMASLN
                     ERAKIDYIVSEGLYNFKLFKDEAERREKVERALRDVGLLPEFATRFPHEFSGGQRQRI
                     GIARALVMEPELLIADEPISALDVSIRAQVINLMNRLKREKSLTYFFIAHDLSVVRFI
                     SDRIAVIHRGRIVELAGAEELFARPLHPYTRALLSAVPVPDPDAEAGKRLTTYDPARA
                     HDYSREAPAWREALPGHFVLGTEGETAGWVGGSSGTP"
     gene            3487..>4086
                     /locus_tag="P5313_12790"
     CDS             3487..>4086
                     /locus_tag="P5313_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81289.1"
                     /translation="MNILVCNVGSTSLKYQLIAMGDEAVLAKGKMERVGTDRAEYSHE
                     DAEGNRIREELPLRDHGEGIERMLRALIGPVVPSLKAVDCVGFKVVLAKDITGVQELT
                     EEVVAAMEAMNGIAPAHNPPYIAAIRQFRRIVPSVPLVGSFETGFHRNLPPKAYLYSI
                     PKEYALRGVRRYGYHGASHEYLAGRAAELGAALEAAKARL"
     assembly_gap    4087..4096
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4097..4734
                     /locus_tag="P5313_12795"
     CDS             <4097..4734
                     /locus_tag="P5313_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRY81290.1"
                     /translation="AAELTGRRDLKLVTCHLGGSGSLAAVDSGVSIDTTLGFSLQCGI
                     MHNNRCGDIDPYLPIYLQETEGLSLAEVKTLLTKKSGLLGLSGVSNDLRDIEQAADSG
                     NRDAREAVEAYAYYIRKQIGAYAAALGGLDAVAFGGGIGENSARVRELSLEGLGFLGL
                     DLDRERNRTARCDAEISTAESRVKVFILATNEEIVVARKAKEFLEARRLPE"
     gene            4802..6895
                     /locus_tag="P5313_12800"
     CDS             4802..6895
                     /locus_tag="P5313_12800"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRY81291.1"
                     /translation="MADTGRRTATIPGRFILLLLLASVPFIGAAVFSTLWTARASGEL
                     IRNQLQNYTSTLHAVIDTLLKANVQTYLRSKVEVGVDALRAVLREHPGDGAARKTGLE
                     QAVGELLNYKVGVSGYYYALDLEGNVVFHPDAAIVGTSQAGKEPIDTQVVRRTGYFEY
                     MWQNTYEEAPRRKALYMEFIPELGWILTATSYRDEFTRMIDLEGLRSAVNSVVFGQSG
                     YSYIVDREGLVISHPYLQGEYVRDHFSPEEGELLMSRFFSAGSGYTTYPWKDPGSRRT
                     RVKMVNHKQLEDFGWVVATSMYVDEMNRPLLVLAGTNAAIALAAALALAAAVYFITRS
                     IDRPMLQLIEVLRRSSAGDLSVRAEPAGLRELDEVADHLNYFIQALHDKIAALGNLTA
                     GIAHELNSPLGAIRSTARSMEANMRRCLEDQQVILAGLSPGERVEYYALLDRARLDPA
                     KPDRLLDRELRRRLETYLSNRGLPGREDLVDALISLGAGDDPEALGTWLSSDRGIERL
                     AAASRVMENFYSVRLIDQASRTAAKVVDTLRNYIYTGEEGAVERLSASQEVELVLTQF
                     QGRIREGLEVRRDYDPQAVLEGDRKRLSVVWNALIKNALDSMGNSGVLGVRTSADGGR
                     ARVEISDTGPGVDPAIRDRIFEPFVTTKTGGAGMGLGLDIARKIVQEYGGTIEFESVP
                     GRTTFRVTFPKPGAP"
     gene            6895..7290
                     /locus_tag="P5313_12805"
     CDS             6895..7290
                     /locus_tag="P5313_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY81292.1"
                     /translation="MRKVILCVDDEAVILLTLKFLFKGAFGNEYRIESAYGAEEALRI
                     LEEEDREEIDVRLLITDWLMPGRKGDDLIRSARAMKPGLPCILITGQAEEDVLAALVR
                     EDLVHAIFRKPWHENQLLDAVRECLDEKR"
     gene            7277..8521
                     /locus_tag="P5313_12810"
     CDS             7277..8521
                     /locus_tag="P5313_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY81293.1"
                     /translation="MKRDESPKMSRPGARGILFWIILAALAPGAFSQTRLFDYLDNHT
                     SVMLLVDPDTGIIEDANKAAAEFYGYPADTLRGMPITRINVLSSPEIQAEMRAAASQR
                     RNYFVFPHRRADGTIRTVEVYSSPVTAPAYGRPLLLSIIHDITGKSVPEAEMDAYRIR
                     LNSLVETRAREVVEARSMNIVLAAAAIVASLTGLALYLGVRSQRRAAETVQAALEDRN
                     KLFAELQHRVKNSFATIVSLIHIDQSRAEDPAVRSALETLGGRVETLSALYRLMHEGR
                     RDEEIDLPEYLMEIARSISSAMGAGGPRLEFDNRIEHLVWGAKGASSLGLILNELVTN
                     AYKYAPEGNPGAVRIRLERIDEDLLLLVSNPGGPLPEGFTPGRGSGFGLLMITELVRQ
                     FQGSLDVRSSNGRVEFSVRIPA"
     gene            complement(8537..>9002)
                     /locus_tag="P5313_12815"
     CDS             complement(8537..>9002)
                     /locus_tag="P5313_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY81294.1"
                     /translation="EFGERPDVLLCPVGGGGLTSGTALSARYFGGGVRVVAAEPAGAD
                     DAFRSFTTRTFVPQTGPKTIADGLLTSLGKLTFPIILDAVSEIVTVSEESIVEAMRLL
                     WERCKLVVEPSGAVPLAALLEGKGAPEGSTVALILSGGNVDLDRLPWTAART"
BASE COUNT         1513 a         3024 c         2974 g         1481 t
ORIGIN      
        1 gcccatggtc ttctacgagg accgccccgt ctcctgggtc ctccccacga tctccctggc
       61 cctctcgggg atcgcctggt acgccatctg gctccggcgc ttcatggtgg acgaggagaa
      121 caaggactac gtcaagttcg cggtctccaa gggactgccg tggaaacggg tcatgcgggg
      181 ccacgtgctc cggaacgccc tggtccccct ggtccagtac ttcccccagc agatcctcct
      241 gaccatcgcg ggatccctcc tgatcgaaag catttattcg atccccggca tgggaggcct
      301 cctggtcagc gccatccgcc agcaggacaa tcccctggtc caggccctgg tactcctgta
      361 ctcggtgctc ggggtcgtcg gggttttcct gggggacctg ctcatggtcc tggtggatcc
      421 ccggatcaag ctggccgaat cgtccggaaa gggggccgcc gcatgatccg aacgattccc
      481 gaggtacccg ccgaactctt ccgacccgcg gccttcgacg aggcggccgc ggagcgcacg
      541 gggtacgccc ggtattccta ctggcggagc accttccgca cctttctcaa gagcccggtc
      601 tcgatcttcc tcctggccct catcgtggtc ctgacgacgg ccagcttcgt ctatcccctg
      661 ttcgcccaca tggacctcgg agcggtgaac ctggacaccg agacctggaa cctgagcccg
      721 aatcgggacc acgtcttcgg gaccgacggc ctgggccggg acatctgggc cctgacctgg
      781 tacgggaccc ggacttccct gctcctgggc ttcgtgatcg cccttttgga tgtcgggatc
      841 gggatgatcg ccggggccct ctggggctac gtccggaagc tcgaccggat catgacggag
      901 ctctacaacg tcctcaacaa catcccccag acggtcttcc tggtcctcct ggcctacatc
      961 atgcggccgg gattctggac catcgtgacg gccctctgct ccaccggctg gatcggcata
     1021 gcccgcttcg tgcggaacaa ggtcatcacc atccgggacg cggagtacaa cgtggcctcc
     1081 cgctgcctgg gcacccccct ccgccggatg atcacgaaga acgtcctgcc ctacctggta
     1141 tccatcctga tcatggaggc ggccctgacc atcccgtact ccatcggttc cgaggtcttc
     1201 ctgggcttca tcggcctggg actccccatc gacaccatct ccctgggaaa cctggtgaac
     1261 cagggaaaga acaacttcat gctctacccc taccagctga cctggccgac cgtgatcctg
     1321 gcggccatca cggtctcctt ctacatcgtc gggaaccggt tctccgacgc ctccgacccg
     1381 aggaaccacg tatgagcggc gacaccatcc tggaggtccg ggacctccgc gtgagcttct
     1441 ccttacgggg ccgacggctc actgccctgc ggggcgccgg cctggacctg cgcaaggggg
     1501 agaccctggc cgtggtcggg gaatctgggt ccgggaagag cgtcctggcc aagaccttcc
     1561 tggggatgct ggaccggaac ggccgcgtgg actccgggtc catcctcttc gacgggaagg
     1621 acctggcccg ctacaacaag gagcgcgagt ggctgggcat ccggggcaag cgcatcgcca
     1681 tgatcttcca ggatcccatg acggccctga accccctcaa gaccgtgggc gagcagatcc
     1741 gggaagctgc ggccctgcac cggggtctgg accgggtcgc ctcccgggcg gtggtcctgg
     1801 agaccctgtc ccgggtgggc ataccggaac cggaactccg gtatcgccag tacccccacg
     1861 agttctccgg ggggatgcgc cagcgggcgg tgatcgccgc ggccgtggcc tgccgtccgg
     1921 agatcctgat ctgcgacgag ccgaccacgg ccctggacgt cacggtccag gcccagatcc
     1981 tggacctgat caagtccctc cagcgggagc tcgggatgac ggtcctcttc atcacccacg
     2041 acctcggggt cgtggccagc gtggcccagc gcgtcgcggt catgtattcc ggccaggtca
     2101 tcgaggaggg gagcgtgcgc gaggtcttct acgatccccg ggccccctac acctgggccc
     2161 tcctggcctc cctgccccag ctgggggtca agggcgagga gctctactcg atcaagggaa
     2221 ccccgcccag cctcttcaag gaggtcgtcg gggacgcctt cgcgccccgg aacccccggg
     2281 ccctggaggt ggacttcaag cacgagcccc cggtcttccc ggtctcggac acccactggg
     2341 cccgaacctg gctcctggat ccccgggcgc cccgcatcga ggctcccgag accgtacgcc
     2401 gcctgcgggc ccgtctggcc cggggcggag ccggcgccgc cgcgacgggc ggcgcggata
     2461 ccgggaaggg aggcgccgaa tgagccggga tgtcctgctt tccgtacggg atctccgggt
     2521 cgactttccc gtcgaccgca agacgttcac cgcggtccgg gacgtgtcct tcgacgtcta
     2581 ccgcggggag accttctccc tggtggggga gtccgggtcc ggcaagacca ccatcggccg
     2641 ggcgatcatg cgcatccacc cggcctcctc gggggaggtc ctcttcgagg gtcggcgcgt
     2701 ctccggccgg atcgacgcgg cctcggagcg gctcctccgg cagaggatcc agatgatctt
     2761 ccaggatccc atggcctccc tgaacgagcg ggccaagatc gactacatcg tctccgaggg
     2821 tttgtacaac ttcaagctct tcaaggacga ggcggaacgc cgggagaagg tggagcgggc
     2881 cctccgggac gtcggactgc tgccggagtt cgccacccgc ttcccccacg agttctccgg
     2941 gggccagcgc cagcgcatcg ggatcgcccg ggccctggtc atggaacccg agctgctgat
     3001 cgcggacgag cccatatccg ccctggacgt ctccatccgg gcccaggtca tcaatctgat
     3061 gaaccggctg aagcgggaaa agagcctcac gtactttttc atcgcccacg acctctcggt
     3121 ggtgcgcttc atctcggacc gtatcgccgt catccaccgg ggccgcatcg tggagctggc
     3181 cggggcggag gagctcttcg cccggcccct ccacccctac acccgggccc tcctgtccgc
     3241 ggtgcccgtg ccggatcccg acgccgaggc gggcaagcgg ctcacgacct acgatcccgc
     3301 ccgggcccac gactactccc gggaagcccc cgcctggcgg gaggccctgc cgggccactt
     3361 cgtcctgggc accgaggggg agacggcggg gtgggtcgga gggtccagcg ggactccctg
     3421 agctcggcgg atattcctcg cggaagggtg gaaggatctg ctatactccc cgaccaagga
     3481 gacgccatga acatcctcgt ctgcaacgtg gggagcacct ccctcaagta ccagctgatc
     3541 gccatggggg acgaggccgt cctggccaag ggcaagatgg agcgggtcgg gacggaccgg
     3601 gccgagtact cccacgagga cgccgaggga aaccgcatcc gggaggagct tcccctgcgg
     3661 gaccacggcg aggggatcga gcggatgctc cgggccctga tcggtcccgt ggtgccgtcc
     3721 ttgaaggccg tcgactgcgt gggcttcaag gtggtcctgg ccaaggacat cacgggggtg
     3781 caggagctca ccgaggaggt cgtggcggcc atggaggcca tgaacgggat cgccccggcc
     3841 cacaatcctc cgtatatagc ggcgatccgg cagttccggc gcatcgtccc ctcggtgccc
     3901 ctggtcggat ccttcgagac ggggttccac cggaacctcc cgcccaaggc ctacctctac
     3961 tcgataccca aggaatacgc ccttcggggg gtgcggcgct acgggtacca cggggccagc
     4021 cacgagtacc tggcgggccg ggccgccgaa ctgggcgcgg cgctcgaagc cgcgaaggcg
     4081 aggctcnnnn nnnnnngggc cgccgaactg acgggccgga gggatctcaa gctggtcacc
     4141 tgccacctgg gagggtcggg ctccctggcg gccgtggact cgggggtctc catcgacacg
     4201 accctgggat tctccctcca gtgcgggatc atgcacaaca accgctgcgg ggacatcgac
     4261 ccctaccttc cgatctacct ccaggagacc gagggcttga gtctggctga ggtcaagacc
     4321 ctcctcacca agaagagcgg cctcctgggc ctgtccgggg tatcgaacga cctacgggac
     4381 atcgagcagg ccgcggactc ggggaaccgg gatgcccggg aagccgtgga ggcctacgcc
     4441 tactacatcc gcaagcagat cggggcctac gcggccgccc tgggcggttt ggacgccgtg
     4501 gccttcgggg gcgggatcgg ggaaaacagc gcccgggtcc gggagctttc cctggaaggc
     4561 ctggggttcc tgggactgga cctggacagg gagcggaacc ggacggcccg ctgcgacgcg
     4621 gagatctcga ccgccgagtc ccgggtcaag gtcttcatcc tggccaccaa cgaggagatc
     4681 gtggtggccc ggaaggccaa ggagttcctg gaagcccgaa gactcccgga atagccggcc
     4741 gggacggcgc cgaagtcccg tttttcttgt gcgggagtac ccgattgtct atacttttcc
     4801 cgtggcggac acagggcggc gcacggcgac aataccgggc aggttcatcc tcctcctgct
     4861 cctggcttcc gtgcccttca tcggggcggc ggtgttctcc accctctgga ccgcccgggc
     4921 ctcgggagag ctgatccgga accagctcca gaattacacc tctaccctgc acgcggtcat
     4981 cgacaccctg ctcaaggcca acgtccagac ctacctgcgg agcaaggtgg aggtcggggt
     5041 ggacgcgctc cgggcggtct tgcgggaaca tcccggggac ggcgcggccc ggaagaccgg
     5101 gctcgagcaa gcggtggggg agctcctgaa ctacaaggtc ggagtttccg gctattacta
     5161 cgctctcgac cttgaaggga acgtggtctt tcacccggat gccgcgatcg tggggaccag
     5221 ccaggccgga aaggaaccga tcgacaccca ggtggtccgg aggaccgggt acttcgagta
     5281 catgtggcag aacacctacg aggaagcgcc tcgccgaaag gccctgtaca tggagttcat
     5341 tccggaactc gggtggatcc tgaccgccac gtcctatcgg gatgagttca cgaggatgat
     5401 cgacctggag ggtttgcgat ccgcggtcaa ttccgtcgtc ttcgggcaga gcggatactc
     5461 ctacatcgtc gatcgggaag gattggtgat ctcccatccc tatctgcagg gagagtacgt
     5521 gagggatcat ttctcccccg aggagggcga gctgctcatg tcccgcttct tctccgcggg
     5581 gtccggctac accacctatc cctggaaaga tccgggaagc cgcaggaccc gggtgaagat
     5641 ggtcaaccac aagcaactgg aggacttcgg ctgggtggtc gccacctcca tgtacgtgga
     5701 cgagatgaac cgacccctcc ttgtcctggc cggtacgaac gcggcgatcg cccttgccgc
     5761 ggccctggcc ctggcagcgg cggtctattt catcacccgg tccatcgacc gccccatgct
     5821 ccagttgatc gaggttctgc gcaggtcctc cgccggggac ctttcggtgc gcgcggaacc
     5881 cgcgggtctg cgggaactgg acgaggtggc ggatcacctc aactatttca tccaggctct
     5941 ccacgataag atagccgcac tgggcaacct gaccgcgggg atcgcccacg agctcaattc
     6001 ccccctgggg gccatccgtt cgacggcccg gtccatggag gcgaacatga ggcggtgcct
     6061 ggaggatcag caggtcatcc tggcgggcct gtccccggga gagcgggtgg aatactacgc
     6121 gctcctggac cgcgcccggc tcgatcccgc gaagccggat cgcctgctcg accgggagct
     6181 caggaggcgc ctggaaacct acctgtcgaa ccggggactc ccgggtcggg aggatctcgt
     6241 ggacgcgttg atcagcctgg gagccgggga cgatcccgaa gccctgggta cctggctttc
     6301 ttcggatagg gggatcgagc ggctggcggc ggcctcccgg gtcatggaga acttctactc
     6361 ggttcgcctg atcgatcagg ccagcaggac ggcggccaag gtcgtggata ctcttaggaa
     6421 ttacatctat acgggggaag agggggccgt ggaacggcta tccgcctccc aggaggtgga
     6481 actggtcctg acccagttcc agggcaggat ccgggagggg ttggaggtcc ggcgggacta
     6541 cgatccccaa gccgtcctgg aaggggatcg caaacgcttg agcgtggtct ggaacgccct
     6601 gatcaagaac gccctggatt cgatggggaa ctcgggcgtc ctgggcgtcc ggacctcggc
     6661 ggacggaggc cgggcccggg tggagatctc ggacaccggt cccggggtag atccggcgat
     6721 ccgggaccgg attttcgagc ccttcgtcac caccaagacc ggcggggcgg gcatgggcct
     6781 ggggctcgac atcgcccgga agatcgtcca ggaatacggc ggaaccatcg agttcgagag
     6841 cgttccggga cgcacgactt tccgggtcac cttcccgaag ccgggagccc cctgatgcgc
     6901 aaggtcattc tctgcgtaga cgacgaggcg gtcatcctcc tgaccctaaa gttcctcttc
     6961 aagggcgcat tcggcaacga gtaccggatc gagtccgcct acggagcgga ggaggcgctg
     7021 cggatcctgg aggaggagga ccgggaagag atcgacgtgc gcctgctcat caccgattgg
     7081 ctcatgccgg ggcgcaaggg ggacgacctc atccggtccg cccgggcgat gaagccgggc
     7141 ctgccctgca tactgatcac gggacaggct gaggaggatg tcctggccgc cctggtgagg
     7201 gaagacctgg ttcacgccat cttccgcaag ccctggcatg aaaatcagct tttggacgcc
     7261 gttcgggagt gcctggatga aaagagatga gtcgccgaag atgagtcgcc caggggcgcg
     7321 cgggatcctg ttctggatca tcctggccgc gctcgctcca ggggcgtttt cccagacccg
     7381 gctcttcgac tatctggaca accacacctc cgtcatgctc ctggtggatc cggatacggg
     7441 gatcatcgag gacgcgaaca aggccgccgc ggagttctac ggatatcccg ccgataccct
     7501 aaggggcatg cccatcaccc ggatcaacgt gttgtcgtcc ccggagatcc aggcggagat
     7561 gagggccgcg gcgagccagc gacggaacta tttcgtgttc ccccatcgcc gggcggacgg
     7621 cacgatccgg accgtggagg tgtattcctc gccggtgacc gccccggcct acgggcgacc
     7681 gctccttctg tccatcatcc acgacataac cggaaagagc gtgcccgagg cggagatgga
     7741 cgcctaccgg atccggctca attccctggt ggagacccgg gcccgggagg tggtggaagc
     7801 ccggtccatg aacatcgtcc tggccgcggc cgcgatcgtc gcgagcctga ccggcctcgc
     7861 gctctatctg ggcgtgcgaa gccagcgccg ggccgcggag acggtccagg ccgccctgga
     7921 ggatcgaaac aagctcttcg cggaactcca gcaccgggtc aagaactcct tcgcgaccat
     7981 cgtctccctc atccacatcg accagtcccg ggccgaggat cccgcggtac ggtccgccct
     8041 ggaaacgctg ggagggcggg tggagactct gtcggccttg taccgcctga tgcacgaggg
     8101 gcggagggac gaggagatcg acctgccgga gtacctcatg gaaatcgccc ggtccatctc
     8161 ctccgcgatg ggagccggcg gcccccgtct ggagttcgat aaccgcatcg agcacctggt
     8221 ctggggcgca aagggagcct ccagcctggg cctcatcctc aacgaactcg tgaccaacgc
     8281 ctacaagtac gccccggagg ggaatcccgg cgccgtccgg atccggctgg agaggatcga
     8341 cgaggacctc cttctcctgg tatcgaaccc cggaggcccc ctgcccgagg gcttcacccc
     8401 ggggcgggga agcggattcg gccttcttat gatcacggag ctggtgcgac agttccaggg
     8461 ttccctggac gtccggtcct cgaacggccg ggtggagttc tcggtccgga ttcccgccta
     8521 ggagcccgac aaactcctag gtccgggcgg cggtccaggg caggcggtcc aggtccacgt
     8581 tcccgcccga gaggatcagg gccaccgttg aaccttccgg tgcgcccttg ccctccagga
     8641 gggcggccag gggcacggcc ccggaaggct ccaccaccag cttgcaccgc tcccacagca
     8701 ggcgcatcgc ctccacgatg gattcctcgg acaccgtgac gatctcggaa accgcatcca
     8761 ggatgatggg gaaggtgagc ttaccgaggc tcgtgagcag gccgtcggcg atggtcttcg
     8821 ggcccgtctg ggggacgaag gtcctcgtgg tgaaggaccg gaacgcgtcg tccgcccccg
     8881 cgggctccgc ggcgaccacc cggaccccgc ctccgaagta ccgggccgag agggccgtac
     8941 cgctggtgag cccgcccccg ccgacgggac acaaaaggac gtcgggcctc tccccgaatt
     9001 cc
//
LOCUS       DAOTEI010000148         8834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5627_length_8834_cov_5.381251, whole genome shotgun sequence.
ACCESSION   DAOTEI010000148 DAOTEI010000000
VERSION     DAOTEI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5627_length_8834_cov_5.381251"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /gene="ccsA"
                     /locus_tag="P5313_12820"
     CDS             <1..465
                     /gene="ccsA"
                     /locus_tag="P5313_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRY81295.1"
                     /translation="SSPLIPKAALPPIPALQSGWLLLHVSFSFIGEAFFAASFVSALA
                     FLFTKDEARRLDLDRITYTAIAVGYPIFTAGALAFGAIWAEQAWGRWWSWDPKETWAL
                     VTWLVYTIYLHVRLILKRKDSWPSILAVAGFLCTVFTFFGVNYLLPGLHSYG"
     gene            643..1683
                     /locus_tag="P5313_12825"
     CDS             643..1683
                     /locus_tag="P5313_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017927852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY81296.1"
                     /translation="MSRRTLTAIVAALVLLPSLAFGQTYTLKLAHLNPQNPFEVASAA
                     MAEVFKSEVEANSNGRIKVELYPNGVLGKEPETMIQVKSGVVQSYISSSGGIAQFYPL
                     IDVTNMPFAFSSYNVGYKVYDGAFGQALAKDIEAKAGFKVLGFGESGGFFAITNSKKP
                     IKSPADMRGIKLRTMALPLHQEIVKALGASPTTVAWAEVYTSLQTGVVDGQMNPISII
                     AMAKFQEVQKYITLTNHLYAPYVWVINPKFFNDLPADLKAVVTDAARTGLLAGRGLSR
                     IIDASDKGLPTLATKMEVYVPTTAEMKQFRDATMPAARKFMSDKYKKEGENWVDQFYK
                     AIEQAEKELAGK"
     gene            1786..2322
                     /locus_tag="P5313_12830"
     CDS             1786..2322
                     /locus_tag="P5313_12830"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY81297.1"
                     /translation="MNSDSGGPGAALERISRGVDALASAICAVFFGAMTAAVLTGVFF
                     RYILNSPLSWTEEVSRYLMIWGASVGISLGIRADEHVGLTILLDSIKSRAVRMVFHTV
                     IFILVLGFLGILFLYSVRMALDARYMQTQALGVPMYVAYAAVPVSSALAAVQLALMYG
                     LKAVRGDQQHRDLKILDI"
     gene            2333..3616
                     /locus_tag="P5313_12835"
     CDS             2333..3616
                     /locus_tag="P5313_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY81298.1"
                     /translation="MFLSIVIVFFALLAIGVPIGFTLGLSGILGLYLMGSGVTLFSMA
                     PLQYFSGLDMFTLMAMPFFIFAGEIMNKGGITQRLVRFSNILAGNIRGGLAHANIIAS
                     VFFAGMTGAAVSDTAAIGTMLIPAMKDDGYDLDFSAAVTAASSIIGPTIPPSNMMVIY
                     GSLMNVSIAGLFAAGFLPGLVLAALLMVMTAYLSWKRGYPKGLKTTFKEKILGFKDAI
                     IPLLMPVIILGGILSGIFTPTEAAAVAVFYAMIVGFFVLKTITPKDLVPMLIKTARIT
                     GSVFLIIGAAGILSWVLAINQVPQAIAAFLLETTGNPKIILLLILILMLIVGMFMDIA
                     AALIILGPILHPIAMSIGMAPIHFGIVMVLSLNIALMTPPVGACLFVACSISKLSLWQ
                     LSKAIWPYIIMEVLALFIVAYVPEISLFLPRLFGF"
     gene            complement(3747..4445)
                     /locus_tag="P5313_12840"
     CDS             complement(3747..4445)
                     /locus_tag="P5313_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81299.1"
                     /translation="MAFTFVPIVLFFIVSIAVTVLLIPKMLQGMRRGREEARDQAGRL
                     GLEYVDGIDALRRSYIDSGQTKALEAFDRMPAFLRSFAQSAAPWRMEGTRKGVRVSVY
                     RETRSSGKSSRTYVVAKAFYPAPLPFKFRAAREGTMAKIGKALFGLRDVQIGDPDFDP
                     KVRIRAEDEAAAASLFFREAAASSFLALLERFPDAVADSSCVTRERQASLLPDGELRE
                     VLDLLAAFAAEAKV"
     gene            complement(4513..5721)
                     /locus_tag="P5313_12845"
     CDS             complement(4513..5721)
                     /locus_tag="P5313_12845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY81300.1"
                     /translation="MAIARGIRENLGRASWIRRTFEEGRRLKEKFGTDAVFDFSLGNP
                     DVEPPPEFHETILRVLREGGKGLHAYMPNAGYPFAREAVARKVSREHECPVSPDHVIL
                     TVGAAGALNVLFHTILDPGDEVVVPRPYFGEYYFYTLNHGGKMVTVDCRPDFELDPQR
                     IAAALGPRTAALIVNTPNNPTGRIYRKETLAELAAVLEEHGRRVGRLPLLVVDEPYRD
                     IVYDERKAPPILGLYPETAVVSSWSKTLSIPGERIGYLALSDSCSGIDEMMDGLVMSN
                     RTLGFVNAPALLQRAVAPLEAKTADLSRYAERRKALAAILDAAGIPYAEPEGAFYFFC
                     RVPEGGPSGSEAERERAFNQCLRNENILAVPGVGFDYPGWFRLSYCVPEATIAGSEAA
                     WKRALLEWNR"
     gene            5937..6935
                     /locus_tag="P5313_12850"
     CDS             5937..6935
                     /locus_tag="P5313_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRY81301.1"
                     /translation="MFQKQIIMRRVLYSLAPVWALSVWLYGPRVVVLTAVSLALGVLT
                     EYLYEKRRNGKVSEAVLVTATLFALSLPPAAPVWIAAAGIVFAVFTAKMIYGGFGRNI
                     YNPAIAGRLFVYISFATVLGRSFTPHGNFGLGAGTLLGRPEVLSAATPLAVWRSGGNV
                     PVLNLLTGLRDGSAGESSALLIAAAAVFLIWTKTASWRIIVSEVAAGGALALALHFAG
                     VKAALPPASLLAGSFLFVAVFMGTDPVSAPKKPASHWIYGALIGITAIVIRTFSAFPE
                     GTSFAVFVGNTFASMIDDIVVGRMNARKARAEAKAAAGGTGSGAAPEAGAGKEAAK"
     gene            6932..7564
                     /locus_tag="P5313_12855"
     CDS             6932..7564
                     /locus_tag="P5313_12855"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRY81302.1"
                     /translation="MKKDSIVYVVVFTFVVCAAFVFFLALANEATKDRVAANRLFAER
                     AAVLDAFGIEYSAPPEALEKYERFVKDQPVPEGAGAEKAYRYEADGKTFFAARVTGAG
                     LWGSISIILGADAGVERLTGLQIVAQNETPGLGGRIDEAWFRGQFRGERIGPEGIRVA
                     TGAESRGTGDPDPDNGLLDGVSGASRTSQSMEAIVNGGIRALRAIAGGAR"
     gene            7561..8211
                     /locus_tag="P5313_12860"
     CDS             7561..8211
                     /locus_tag="P5313_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRY81303.1"
                     /translation="MSGKAGKILWDNLWTNNPIFIQILGICSTLAVTNSVRNTLIMTV
                     GVSLSTAFSSWTLSAMQATIPRKVRMIVQVLVISFYVIIIDIILRAYLPEVSKQLGPY
                     VGLIITNCIIMGRAEAFAAANKPVLSFWDGLTSGLGYMAVLVAIAVVREILGFGTLLG
                     FRVMPAGFEPWTIMIMAPSAFFLVAFVMWWAKTVQARAAAKAAAAKSAPRPVPAKA"
     gene            8222..8821
                     /locus_tag="P5313_12865"
     CDS             8222..8821
                     /locus_tag="P5313_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRY81304.1"
                     /translation="MNAEPLVNPVVLFFASIFTSNILLSNFLGMCSFISISKDKTSAY
                     GLGLAVTVVMTICAAVSWLVLRYLILPLDIEYLSFIIFIIVIAAVVQMLEMIIDRVSP
                     ALYMSLGIFLPLITVNCAILGAILFMQIRSYGFLQAVAFGAGSGMGWWLAIMLLASIR
                     KRIENNPVPAGLKGAGITMVTIGFMAMAFIGFSGMIAVQ"
BASE COUNT         1430 a         3060 c         2762 g         1582 t
ORIGIN      
        1 tcctcccccc tgatccccaa ggcggccctt ccccccatcc cggccctcca gtcggggtgg
       61 ctgctgctgc acgtgtcctt ctccttcatc ggggaggcct tcttcgcggc cagcttcgtg
      121 tccgccctgg ccttcctgtt caccaaggac gaggcccggc gcctggacct ggaccggatc
      181 acctacacgg ccatagccgt ggggtacccg atcttcacgg cgggagccct ggccttcggg
      241 gccatctggg ccgagcaggc ctggggacgg tggtggagct gggatcccaa ggagacctgg
      301 gccctggtga cctggctggt ctacacgatc tacctgcacg tccgcctcat cctgaagcgc
      361 aaggactcct ggccttccat cctggcggtg gccgggttcc tgtgcacggt gttcaccttc
      421 ttcggggtga actacctcct gcccggcctg cacagttacg gctaagcgtc cggcgggctt
      481 ctgcccgccg gacggcacgg aagcttcgct tccgcgatcc tcaggaaccg acctcgaggc
      541 aaggcccgta gaattctgca tacattttct gacaaaatcg ataattgacg gatcggaaat
      601 caggacatat actcaatgtc ttcacgttac caggaggtac tcatgtctcg cagaaccctg
      661 accgccatcg tcgcggccct cgtacttctc ccgtccctgg ccttcgggca gacctacacc
      721 ctcaagctcg cgcacctgaa tccgcagaat cccttcgagg tggcttccgc ggccatggcc
      781 gaggtcttca agagcgaagt cgaagccaac tccaacggcc ggatcaaggt cgaactctat
      841 cccaacggcg tcctgggcaa ggaacccgag accatgatcc aggtcaagtc cggggtcgtc
      901 cagtcctaca tctcctcctc cggaggcatc gcccagttct atcccctcat cgacgtgacc
      961 aacatgccct tcgccttctc cagctacaac gtgggctaca aggtctacga cggcgccttc
     1021 ggccaggccc tggccaagga catcgaggcc aaggcgggct tcaaggtcct gggcttcggg
     1081 gaatccggcg gcttcttcgc catcaccaac tccaagaagc cgatcaagag cccggcggac
     1141 atgcggggca tcaagctccg caccatggcc ctgcccctgc accaggagat cgtgaaggcc
     1201 ctgggcgcct ccccgaccac cgtggcctgg gccgaggtct acacctccct gcagaccgga
     1261 gtcgtggacg gccagatgaa ccccatctcc atcatcgcca tggccaagtt ccaggaagtc
     1321 cagaagtaca tcaccctgac gaaccacctc tacgccccct acgtctgggt catcaacccc
     1381 aagttcttca acgacctccc cgcggacctc aaggccgtcg tgaccgacgc cgcccgcacg
     1441 ggcctcctgg ccggccgggg cctgtcccgc atcatcgacg ccagcgacaa gggactcccg
     1501 accctggcca ccaagatgga ggtctacgtc cccaccaccg ccgagatgaa gcagttccgg
     1561 gacgccacca tgcccgcggc ccgcaagttc atgtcggaca agtacaaaaa ggaaggcgag
     1621 aactgggtgg accagttcta caaggccatc gagcaggcgg agaaggagct ggccgggaag
     1681 tgacccgggc cctgatccta cgaattaaaa aagggacggc gcccggactt cgggtgccgt
     1741 cccttgtctt gtacggccgg gcccggggcc cggaaaggaa gcgcgatgaa ctctgactcc
     1801 ggcggaccgg gagccgccct ggaacggatc tcccgggggg tggacgccct ggccagcgcg
     1861 atctgcgcgg tcttcttcgg cgccatgacg gcggcggtgc tcacgggggt gttcttccgg
     1921 tacatcctga attcccccct gtcctggacc gaggaggtca gccgctacct catgatctgg
     1981 ggcgcctcgg tggggatctc cctggggatc cgtgcggacg agcacgtggg gctcaccatc
     2041 ctgctggact ccatcaagtc ccgggccgtc cgcatggtgt tccatacggt catcttcatc
     2101 ctggtcctcg ggttcctggg gatcctcttc ctctactccg tccgcatggc cctggacgcc
     2161 cggtacatgc agacccaggc cctgggagtt ccgatgtacg tggcctacgc ggccgttccc
     2221 gtctcctcgg ccctggcggc ggtccagctc gccctcatgt acggcctcaa ggccgtccgg
     2281 ggggaccagc agcaccggga cctgaagatc ctcgacatct aggagggcgg atatgttcct
     2341 atccatcgtc atcgtgttct tcgccctcct ggccatcggg gtgcccatcg ggttcaccct
     2401 gggcctctcg ggcatcctcg ggctctacct catgggatcg ggggtcaccc tcttctccat
     2461 ggcgcccctg cagtacttct ccggcctgga catgttcacc ctcatggcca tgcccttctt
     2521 catcttcgcc ggcgagataa tgaacaaggg cggcatcacc cagaggctgg tgcgcttctc
     2581 gaacatcctg gccggcaaca tccggggcgg actggcccac gccaacatca tagccagcgt
     2641 cttcttcgcc gggatgaccg gggccgccgt gtccgacacc gccgccatcg gcaccatgct
     2701 gatcccggcc atgaaggacg acggctacga cctggacttc tccgcggccg tcaccgccgc
     2761 gtcctccatc atcggcccca cgatcccgcc ctcgaacatg atggtcatct acggatccct
     2821 catgaacgtg tccatcgcgg ggctcttcgc tgcgggattc ctgccgggcc tggtcctggc
     2881 ggccctcctc atggtcatga ccgcctatct ctcgtggaaa cgcggctatc ccaagggctt
     2941 gaagaccacc ttcaaggaga agatcctggg cttcaaggac gcgatcatcc ccctcctgat
     3001 gccggtcatc atcctgggcg ggatcctctc cgggatcttc acccccaccg aggccgccgc
     3061 cgtggcggtg ttctacgcca tgatcgtcgg gttcttcgtg cttaagacca tcaccccgaa
     3121 ggacctggtc cccatgctga tcaagaccgc ccggatcacg ggatccgtat tcctgatcat
     3181 cggggcggcg ggcatcctgt cctgggtcct ggcgatcaac caggtgcccc aggccatcgc
     3241 ggccttcctc ttggagacca cggggaatcc caagatcatc ctgcttctga tcctgatcct
     3301 gatgctgatc gtggggatgt tcatggacat cgcggcggcc ctgatcatcc tggggccgat
     3361 cctgcacccc atcgcgatgt ccatcggaat ggcgcccatc cacttcggga tcgtcatggt
     3421 cctgtccctg aacatagccc tgatgacacc ccccgtgggg gcctgcctct tcgtggcctg
     3481 ctcgatctcc aagctgtccc tctggcagct gtccaaggcc atctggccct acatcatcat
     3541 ggaggtactg gccctgttca tcgtggccta cgtcccggag atctcgctct tcctgccgag
     3601 gctcttcggg ttctagcagg ttgttgaaat gcacgaggca tttcaacaac ctgctatgca
     3661 attgctcgag aaccttacgg ttctctgcgc aattgcgaca ttaaggtagt gttgaaaaag
     3721 gctctatagc ctttttcaac accctgttag accttcgcct ccgccgcgaa ggccgccagc
     3781 aggtccagga cctccctcag ctccccgtcc gggagcaggg aggcctggcg ttcccgggtg
     3841 acgcaggagg aatccgcgac ggcgtccggg aatctctcca ggagggccag gaaggacgac
     3901 gccgcggctt cccggaagaa gagggaggcc gcggccgcct cgtcctccgc ccggatcctc
     3961 accttgggat cgaagtccgg gtccccgatc tgcacgtccc ttagcccgaa gagagccttc
     4021 ccgatcttgg ccatcgtccc ctcccgggcc gcccggaact tgaagggcag gggcgcgggg
     4081 tagaaggcct tggcgacgac gtaggtccgg gagctcttgc cgctggaccg ggtctcccgg
     4141 tagaccgaga cgcggactcc cttccgggtt ccctccatgc gccagggcgc cgcggactgg
     4201 gcgaaggagc ggaggaaggc gggcatccga tcgaaggcct ccagggcctt ggtctgcccc
     4261 gaatctatgt aggaccggcg cagggcgtcg atcccgtcca cgtactccag gccgagccgg
     4321 cctgcctggt cccgggcctc ctcccggccg cgccgcatgc cctgcagcat cttcgggatc
     4381 agcaggaccg tcacggctat ggagacgatg aaaaagagga cgatgggaac aaaggtaaac
     4441 gccatgcctc aagtataggc ggagggcgcg aaaaagcgag gaccgccgcg gcggccgtgg
     4501 aattcggagg atctaccggt tccactccag gagggcccgc ttccaggccg cctcggagcc
     4561 cgcgatggtc gcctcgggca cgcagtacga gaggcggaac cagcccggat agtcgaaacc
     4621 cacccccggc acggccagga tgttctcgtt ccggaggcac tggttgaagg cccgctcccg
     4681 ctccgcctcc gaacccgagg ggccgccttc cgggacccgg cagaagaagt agaaggcccc
     4741 ctcgggctcg gcgtagggga tgcccgcggc gtccaggatg gccgccaggg ccttccggcg
     4801 ctccgcgtac cgggagaggt ccgcggtctt cgcctcaagg ggggccaccg ccctctggag
     4861 cagggccggg gcgttgacga agcccagggt ccggttgctc ataaccaggc cgtccatcat
     4921 ctcgtcgatc ccggagcagg aatctgacag ggccaggtag cctatgcgct ccccggggat
     4981 ggacagggtc ttggaccagg aggatacgac cgccgtctcg gggtagagcc cgaggatggg
     5041 aggggccttc cgttcgtcgt atacgatgtc ccggtagggc tcgtccacca ccaggagggg
     5101 gaggcgcccg acgcggcggc cgtgctcctc caggactgcg gcgagctccg ccagggtctc
     5161 cttccggtag atgcggccgg tggggttgtt gggggtgttc acgatcaggg ccgcggtgcg
     5221 ggggccgagg gccgctgcga tccgctgggg atccagctcg aagtcgggcc ggcagtccac
     5281 ggtcaccatc tttccgccgt ggttcagggt gtagaagtag tactccccga agtagggccg
     5341 ggggaccacc acctcgtccc cgggatccag gatcgtgtga aaaaggacgt tgagggcccc
     5401 ggcggctccc acggtcagga tcacgtggtc cggggacacc gggcattcgt gctcccggga
     5461 caccttgcgc gccaccgcct cccgggcgaa ggggtagccc gcgttgggca tgtaggcgtg
     5521 cagtcccttg cccccctccc ggaggacccg gaggatcgtc tcgtggaact ccggaggcgg
     5581 ctccacgtcg gggttcccca gggaaaagtc gaagaccgcg tccgtcccga acttctcctt
     5641 gagtcggcgc ccctcctcga aggtcctgcg tatccaggac gcccgtccca ggttttcccg
     5701 aatgcctcgc gcgatcgcca cggtgtccac ctcccgcgcc cgttcccggc cgcctctccg
     5761 gataacggaa agaatcggcc cgggggacgc ggctccattc tcccggctcc gggcggatcc
     5821 gtcaatgaga aagatttcct cgttcccgaa gcccgcggcg cgcgccgggc ttgcccgtcc
     5881 cagtagtgtt tcctatactt aggtcgtcgc atcgggtgga aaaaaaggag atccgcgtgt
     5941 tccagaaaca gatcatcatg cgacgcgtgc tctacagcct ggcgcccgtc tgggcccttt
     6001 cggtgtggct gtacggaccc cgcgtcgtcg tcctgaccgc cgtgagcctg gccctggggg
     6061 tcctcacgga atacctgtac gagaagcgcc ggaacgggaa ggtcagcgag gccgtcctgg
     6121 tcaccgcgac cctcttcgcc ctgtccctgc ctcccgcggc gccggtctgg atcgcggcgg
     6181 cgggcatcgt cttcgcggtg ttcaccgcca agatgatcta cgggggcttc ggacggaaca
     6241 tctacaaccc cgccatcgcg gggcggctgt tcgtctacat ctccttcgcc accgtactgg
     6301 ggcggtcctt cacgccccac ggtaacttcg ggctcggcgc cggaaccctt ctgggccgcc
     6361 cggaggtcct ctccgccgcc acacccctgg cggtctggcg ctccggcggg aacgtccccg
     6421 tcctgaacct cctcacgggg ctccgggacg gctctgccgg ggaatcctcg gccctcctga
     6481 tcgccgcggc ggcggtcttc ctgatctgga ccaagaccgc cagctggcgg atcatcgtgt
     6541 ccgaggtggc cgcgggcggg gccctcgccc tggccctcca cttcgcgggg gttaaggcgg
     6601 ccctgccccc ggcctccctc ctggcgggga gcttcctctt cgtggcggtc ttcatgggca
     6661 cggaccccgt gtcggccccc aagaaacccg cctcccactg gatctacggg gccctgatcg
     6721 ggatcaccgc catcgtcatc cggaccttca gcgccttccc ggagggcacc agcttcgcgg
     6781 tcttcgtggg caacaccttc gccagcatga tcgacgacat cgtggtcggc cgcatgaacg
     6841 cccggaaggc ccgggccgag gccaaggccg cggcgggcgg gacgggatcg ggcgcagccc
     6901 cggaggccgg ggccggcaag gaggccgcga aatgaagaag gactcaatcg tctacgtggt
     6961 ggtcttcacc ttcgtcgtgt gcgcggcctt cgtcttcttc ctggccctgg ccaacgaggc
     7021 cacgaaggac cgggtggcgg ccaaccggct cttcgcggag cgggcggcgg tcctggacgc
     7081 cttcggcatc gagtactccg ccccgccgga ggccctggaa aagtacgagc ggttcgtcaa
     7141 ggaccagccc gtgccggagg gcgccggggc ggaaaaggcc taccgctacg aggcggacgg
     7201 gaagaccttc ttcgccgccc gggtcaccgg ggcgggcctc tggggttcca tctccatcat
     7261 cctgggggcg gacgccgggg tcgagcggct cacgggcctc cagatcgtgg cccagaacga
     7321 gacccccggc ctgggaggcc gcatcgacga ggcctggttc cggggccagt tccggggcga
     7381 gcggatcggc ccggagggca tccgcgtcgc gacgggcgcc gagtcccggg gcacggggga
     7441 tccggacccg gacaacgggc ttctggacgg ggtgtccggg gccagccgca ccagccagtc
     7501 catggaggcc atcgtgaacg gcggaatccg ggccctgcgg gcgatagcgg gaggcgcgcg
     7561 atgagcggaa aagccggaaa gatcctttgg gacaacctct ggacgaacaa tcccatcttc
     7621 atacagatcc tgggaatctg ctccaccctg gcggtcacga actcggtgcg gaacaccctg
     7681 atcatgaccg tgggcgtgag cctctccacc gccttctcca gctggaccct gtcggccatg
     7741 caggcgacca tcccccggaa ggtgcggatg atcgtccagg tcctggtgat ctccttctac
     7801 gtgatcatca tcgacatcat ccttcgggcc tacctgcccg aggtatccaa gcagctggga
     7861 ccctacgtgg ggctcatcat cacgaactgc atcatcatgg gccgggcgga agccttcgcg
     7921 gcggccaaca agccggtcct gtccttctgg gacgggctga ccagcgggct gggatacatg
     7981 gccgtcctgg tcgccatcgc cgtagtccgg gagatcctgg gcttcggcac cctcctgggc
     8041 ttccgggtga tgcccgcggg cttcgagccc tggaccatca tgatcatggc gcccagcgcc
     8101 ttcttcctgg tggccttcgt gatgtggtgg gccaagaccg tgcaggcccg ggcggcggcc
     8161 aaggccgcgg cggccaagtc ggccccccgg cccgtcccgg ccaaggcgta aggaggatac
     8221 cgtgaacgcc gaacccctcg tgaaccccgt cgtcctcttc ttcgcctcca ttttcaccag
     8281 caacatcctt ttatccaact tcctggggat gtgctccttc atctcgatct ccaaggacaa
     8341 gaccagcgcc tacggcctcg ggctggccgt gacggtggtg atgaccatct gcgccgcggt
     8401 cagctggctg gtcctgcggt acctcatcct gcccctggac atcgagtacc tctcgttcat
     8461 catcttcatc atcgtcatcg ccgcggtggt gcagatgctg gagatgatca tcgaccgggt
     8521 gtcccccgcc ctctacatgt ccctggggat cttcctgccc ctgatcacgg tgaactgcgc
     8581 catcctgggc gccatcctgt tcatgcagat ccgcagctac ggattcctcc aggccgtggc
     8641 cttcggggcg ggctccggca tgggctggtg gctggccatc atgctcctgg cgagcattag
     8701 gaagcggatc gagaacaatc ccgtgcccgc gggtctcaag ggggcgggca tcaccatggt
     8761 gaccatcggc ttcatggcca tggccttcat cggcttctcc ggcatgatcg ccgtacagta
     8821 ggaggaacca tgag
//
LOCUS       DAOTEI010000149         8728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5705_length_8728_cov_4.499827, whole genome shotgun sequence.
ACCESSION   DAOTEI010000149 DAOTEI010000000
VERSION     DAOTEI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5705_length_8728_cov_4.499827"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..419
                     /locus_tag="P5313_12870"
     CDS             228..419
                     /locus_tag="P5313_12870"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRY81305.1"
                     /translation="MILPFEQLIENDTNAYEFTVAVTRRAYQLAVLRTPEVEKHNGKV
                     VSLGMRQIFSGQVVYRFIE"
     gene            397..1344
                     /gene="miaA"
                     /locus_tag="P5313_12875"
     CDS             397..1344
                     /gene="miaA"
                     /locus_tag="P5313_12875"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRY81306.1"
                     /translation="MCTASSSRTLLLFGPTASGKTALLSELFLGNRGSGLPQAEVVSA
                     DSMQVYRGLDIGTAKPDAGLLENLPHHLIDIRDPSEQYTAGDFVREADRACDEIRSRG
                     RLPVISGGTAFYLKNFVYGLPDSPPADARIRSEVERDLRLRGPEALRAELEAGDPESA
                     GRIHARDLYRLTRAVEVLRLTGRPLSSFEVPAGPRAEHDLLLIALDRPREELYARIDA
                     RVDAMFESGLAGEVRGLLAAGYGDADPGMQAIGYREFLEARRTGCARLRDVRDRIALD
                     TRRYAKRQLAFFHTLPGVEWFSAEDAEGVRGRIREWVNK"
     gene            1413..1892
                     /locus_tag="P5313_12880"
     CDS             1413..1892
                     /locus_tag="P5313_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HRY81307.1"
                     /translation="MELSTLLATVFLSFLPISELRGAIPFAVARGMPLPAAFLLAVAC
                     NVLVAPVAFLFLGTLHGLLYRWAFYARIFDRFVEKARAKVGGSVEKYGYWGLLVFVAV
                     PLPVTGAWTGALGAWILGLRKRKATLYIALGVLAAGIVVSAVVGLGIGALSFLIKRI"
     gene            1906..3912
                     /locus_tag="P5313_12885"
     CDS             1906..3912
                     /locus_tag="P5313_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY81308.1"
                     /translation="MKIDFQGELDPEQYQAVTAVEGPVLIIAGAGSGKTRVITYRIAY
                     MLEKGIPQSAILALTFTNKAAREMEERVKALTKKKLQSLTVSTFHAFGVKILRKEIHR
                     LGWKDSFSIYDETDRTQLIKDCARETGFRMEGFDPAAAGAAFSEVKTRRRAWDGTDLY
                     EKVYEEYQASLKAHNAVDFDDLIVKPIEVFEAFPDALAKYREQYRYIMIDEFQDTSKI
                     QYRLMRLLADDNVCAVGDDDQSIYSWRGANYENLVQFERDWPHRKEVKLERNYRSTST
                     ILDAANAVIAHNENRKEKALWSPSRTGGTPIEVYTPENEVEEAEFIARAVKEARLSEK
                     VPYSDIGILIRTNALTRNLEEALLAENVPYKVSGGTSFFQRKEIKDVISYLRVIANPQ
                     DDVNLIRILNVPRRGLGRRALEHLQERARRMSGPLFAALESLASDGEAEFPERSRADA
                     LEFLQTIRGYREEMLGHRNLAAKVRKMVDGIDYWGYLVSENQHSEKAAQWKFLNVESL
                     IQSIETWEKDPDNLDPSLFNWLNRISLITRDDLDEEADAGKVNLMTVHASKGLEFEVV
                     FIAGCEDGIMPHARALAEGEGNLEEERRLFYVAVTRARRKLYISACRQRRRQAQAVDC
                     APSPFLAEIPSHLVTYYEEPEVTEEAADAVFAGLRKMWGEGVDR"
     gene            complement(3993..4928)
                     /locus_tag="P5313_12890"
     CDS             complement(3993..4928)
                     /locus_tag="P5313_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRY81309.1"
                     /translation="MIGAIPPGARPLEAAVYHAAPGMTRHLLAELGEWDRQDGDLLLR
                     SGPVLPARWVRNIWTRPFLLEFASLGEAAAALRSVQRNWAPCPARLFRRTALLAEKLP
                     PLPSKPKVFPFDPPPAPMGAFTLLDEGLLLGSAACSSPFPNGELRFEEDTEGPPSRAY
                     RKLWEALVLAGKRPGPGDRCLDAGASPGGWTWALAGLGASVLAVDRAPLEPRIAALPG
                     VEFRKGSAFSLRPEDSDPLDWLFSDVICYPPVLYDWVEKWLDSGRARNFVCTIKMQGE
                     EYDRATTDRFAAIPGSRVVHLWHNKHELTWIKVNE"
     gene            complement(4925..7789)
                     /locus_tag="P5313_12895"
     CDS             complement(4925..7789)
                     /locus_tag="P5313_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81310.1"
                     /translation="MIALYVRIDYHGQHRRMDPPGAADLQTRMERIAADYGARPSGRE
                     HPFVAVFDSSSIFWRLQAIEALLAFRDLLRSRFEGTMDFFAALHEGPDEETLRAEMDR
                     AWAEADRPEDAWLGPAADRETRGYLNLESAGSGLARVLGPSYRLDGLVRTAQEFYRRE
                     KAAANISELIGELVVGGEKNVLILRGAAGTGKEATVAGALEALGYPPDRFLTLRGGTV
                     NPRPFDPLACGITGDFLAEARVHLGSVEGQLYERLLPAYRYVFAGPFRAVLAPAMETG
                     YRLFMGLVLRAYARGMSRAGLPPILVCENLHRFSPESLDLLGELLLSSDIRIVATTAR
                     NCPQSWPEYGILEWDLPPPDFEEYSGKLSEVLPEAPEETRKSLFSRARGDLPLLYRCA
                     LLELEHASGPPPSEAILGLMPREAAAALYAASLCENVLDTPRFHDFLESLGLRAKARQ
                     IVLDLLGRTGYLSGPGPRNASRMSNRRLSAFVGTDAQRIEASLAEYLTGLYRNREAYP
                     SLGLLSATKAFSGEDRQRFEIDCVLSDALEDPQDQSRQAYKDIPPALRKLRELAACLV
                     SGDEARASARLAELEETDSRSTLPPGLPFLFRAELSLARREFPEAAKQAKEALLSARG
                     GPEPKLEARCHRLLGLCALARERPEESIEYFENAHDISGAARDEYERLLCRWFTAVAL
                     FASGRLDKALKLTNQAEAASQAVFRRDWEAASVFLRARIHLELGRYEEARVGFEGVRA
                     IARAYGFDEAGRRADIWDARALAYAGSHEEARTLLSRHEGDGEAEFFRAELELLRGNP
                     GGAAEILEALPPWEHGTFDPAERLFWDSGYRGIERRAFGASALRPFDADLRAAYACFA
                     RGLERREGAWAVQVYRMTREDKLSAEHPAFSTYLFFCFLLLERLAEPPLDKATVLSRA
                     FKYLQERAGRIEDAAAGSDFLERNLWNRRLVEAARRYKLL"
     gene            8027..8521
                     /locus_tag="P5313_12900"
     CDS             8027..8521
                     /locus_tag="P5313_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY81311.1"
                     /translation="MVDAVRSVKLPPLPLNEELAAFSVLELLSNSIRAHRERGVREDV
                     ELRYSISDNRLKIEILDSGRGFDPGRLPYRLDSPPDSIDPRSETFTEYRLKYGNGRFG
                     MGLLAARKTFPAFSLTFVDRDLSPCPWYSGMVRGTRIVLSAPLASEAWAEDLEELPPA
                     EVEE"
     gene            8518..>8728
                     /locus_tag="P5313_12905"
     CDS             8518..>8728
                     /locus_tag="P5313_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81312.1"
                     /translation="MSRERRGTDRNRGFARAGLVPSLYGYVADVSARGFRAVFREDPR
                     LSKGAPVVPVLSFSEIDVEPFPLPSE"
BASE COUNT         1452 a         2942 c         2980 g         1354 t
ORIGIN      
        1 tgtctatatc gtggacgagc tcttccggga acggacgaac cgctcctccc gggaggccga
       61 ccaggacgag ttgggccggg ccgcggaccg gctgatcgag cgtctggagc gggccatctc
      121 ggacctggaa cccggaccgg ggcccgaaag cccgggcgcc ccttgatccg tccgggacgt
      181 cccgatataa ttccagttcc agcaacgaga cgacaagcgg agagcccatg atactaccct
      241 tcgagcagct gatcgagaac gataccaacg cctacgagtt caccgtggcc gtcacccgcc
      301 gggcctatca gctcgccgtg ctccgcaccc ccgaggtgga gaagcacaac ggcaaggtgg
      361 tctccctcgg catgcgccag atcttctccg gccaggttgt gtaccgcttc atcgagtagg
      421 acccttctcc tcttcggccc gacggcgtcg ggcaagacgg ccctcctttc ggaactcttc
      481 ctcggaaacc ggggatccgg cttgccccag gccgaggtgg tctccgcgga ctccatgcag
      541 gtctaccggg gactggacat cggcacggcc aagccggacg cggggcttct cgagaatctt
      601 ccccaccacc tgatcgacat ccgcgatccc tcggaacagt ataccgccgg ggattttgta
      661 cgggaggccg accgggcctg cgacgaaatc cgaagccggg gacgcctgcc cgtgatctcc
      721 ggaggcacgg ctttttacct gaagaacttc gtctacggcc tcccggattc tccccccgcg
      781 gacgcccgga tccggtccga ggtggagcgg gacctccggc tccggggccc cgaggctctc
      841 cgggcggagc tggaggccgg ggatcccgag agcgccggcc gcatccatgc ccgggacctg
      901 taccggctca cccgggccgt ggaggtcctg cgcctgacgg gccggcccct ctcttccttc
      961 gaggttcccg cgggaccccg ggcggaacat gacctccttc tcatagccct ggaccgcccc
     1021 cgggaggagc tgtacgcccg catcgacgcc cgggtggacg cgatgttcga gtcgggcctg
     1081 gccggggagg tccgggggct cctggccgcc ggctacgggg atgcggaccc ggggatgcag
     1141 gccatcgggt accgggaatt cctggaagcc cggaggaccg gttgtgcccg gctccgggac
     1201 gtgcgggacc ggatcgccct ggacacccgc cgctacgcca agcggcagct cgccttcttc
     1261 cataccctgc ccggggtcga gtggttttcc gcggaggacg cggagggggt gcggggaaga
     1321 atacgggaat gggtgaataa gtaaatagag agcctcccat tcacccattc acctattcac
     1381 ctattcactt caacccgcta tactccgccc ccatggaact ctcgaccctg cttgccacgg
     1441 tattcctttc cttccttccg atatcggagc tccgcggggc catccccttc gcggtcgccc
     1501 gggggatgcc cctgcccgcg gccttcctcc tggccgtggc ctgcaacgtc ctggtggcgc
     1561 ccgtggcctt cctcttcctg ggcaccctgc acggattgct gtaccggtgg gccttctacg
     1621 cccggatctt cgaccggttc gtggaaaagg cccgggccaa ggtcggcgga tccgtggaga
     1681 agtacggata ttggggcctt ctggtcttcg tagccgtccc cctgcccgtg acgggagcat
     1741 ggaccggcgc cctgggggcc tggatcctgg gcctccggaa gcgaaaagcc accctctaca
     1801 tcgccctggg ggtcctggcc gcgggcatcg tggtctccgc ggtcgtcggc ctcgggatcg
     1861 gagccctgtc cttcctgatc aagcggatct agcgcgtacc cggacatgaa gatcgatttc
     1921 cagggggaac tcgacccgga gcagtaccag gcggtcaccg ccgtcgaggg gcccgtgctg
     1981 atcattgccg gcgccggatc cggcaagacc cgggtcatca cctaccgcat cgcctacatg
     2041 ctggaaaagg gcatccccca gtccgccatc ctggccctca ccttcacgaa caaggcggcc
     2101 cgggagatgg aggaacgggt caaggccctg accaagaaga agctccagag cctcacggtc
     2161 tccaccttcc atgccttcgg ggtcaagatc ctccggaagg agatccaccg cctgggctgg
     2221 aaggattcct tttcgatcta cgacgagacg gaccggaccc agctgatcaa ggattgcgcc
     2281 cgggagacgg ggttccggat ggagggcttc gatccggccg cggcgggggc cgcgttctcg
     2341 gaggtgaaga cccgcaggcg ggcctgggat gggacggacc tgtacgagaa ggtctacgag
     2401 gagtaccagg cctccctcaa ggcccacaac gccgtggact tcgacgacct gatcgtgaag
     2461 cccatcgagg tctttgaggc cttcccggac gccctggcca agtaccggga gcagtaccgg
     2521 tacatcatga tcgacgagtt ccaggacacc tccaagatcc agtaccggct gatgcggctc
     2581 ctggcggacg acaacgtctg cgcggtgggg gacgacgacc agtccatcta ctcctggcgg
     2641 ggggcgaact acgagaacct cgtccagttc gagcgggact ggccccaccg gaaggaggtc
     2701 aagctggagc ggaactaccg ctccacctcc accatcctgg acgcggccaa cgcggtcatc
     2761 gcccacaacg agaaccgtaa ggaaaaggcc ctctggtccc cctcccgcac ggggggcacc
     2821 cccatcgagg tctacacccc ggagaacgag gtcgaggagg cggagttcat cgcccgggcg
     2881 gtcaaggagg cccggctatc cgagaaggtc ccctacagcg acatcggcat cctgatccgc
     2941 acgaacgccc tgacccggaa cctggaggag gccctcctgg cggagaacgt cccgtacaag
     3001 gtctccgggg ggacgagctt cttccagcgc aaggagatca aggatgtgat ctcctacctc
     3061 cgggtcatcg ccaatcccca ggacgacgtg aacctgatcc ggatcctgaa cgtgccccgc
     3121 cggggactgg ggaggcgggc cctggagcac cttcaggagc gggcccggcg gatgtcgggc
     3181 ccccttttcg cggccctgga atccctggcg tcggacggag aagcggagtt ccccgagcgg
     3241 agccgggcgg acgccctgga attcctccag acgatccggg gctaccggga ggagatgctg
     3301 ggccaccgca acctggccgc caaggtccgg aagatggtcg acgggatcga ctactggggc
     3361 tacctggtct cggagaacca gcacagcgag aaggccgccc agtggaagtt cctgaacgtg
     3421 gagagcctca tccagtccat cgagacctgg gaaaaggacc cggacaacct ggatccctcc
     3481 ctgttcaact ggctcaaccg catatccctg atcacccggg acgacctgga cgaggaggcg
     3541 gacgccggca aggtgaacct catgaccgtc cacgcctcca agggcctgga gttcgaggtg
     3601 gtcttcatag cgggctgcga ggacgggatc atgccccacg cccgggccct ggccgagggg
     3661 gaggggaacc tggaggagga gcggcggctt ttctacgtgg ccgtcacccg ggcccgccgc
     3721 aagctctaca tctccgcctg ccgacagcgc cgccgccagg cccaggcggt ggactgcgcc
     3781 ccgtccccct tcctggcgga gatcccgtcc cacctcgtca cctactacga ggaacccgag
     3841 gtcaccgagg aggccgcgga cgccgtgttc gcgggtctca ggaagatgtg gggggagggg
     3901 gtggacaggt gaataggtga ataggtgaat aggtgaatag gtgaataggt gaataggtga
     3961 atagaatgcc ccatattcac tcattcactc attcactcat tcacctttat ccacgtcaac
     4021 tcgtgcttgt tgtgccacag gtgcacgacc cggcttccgg ggatggccgc gaaacgatcc
     4081 gtggtcgctc ggtcgtactc ctcgccctgc atcttgatgg tgcagacgaa attccgggcc
     4141 cggccggagt ccagccactt ctccacccag tcgtacagca ccggggggta gcagatcacg
     4201 tcggagaaaa gccagtccaa gggatcagag tcctcgggcc tcagggagaa ggcgctgccc
     4261 ttccggaact cgacccccgg cagggcggcg atccggggct ccaggggggc ccggtccacg
     4321 gccaggaccg aggcgcccag gcccgccagg gcccaggtcc agccgcccgg ggaggccccc
     4381 gcgtccaggc agcggtcccc cggtcccggc ctcttgcccg caaggaccag ggcttcccac
     4441 agcttgcggt aagcgcggct ggggggaccc tccgtgtcct cctcgaagcg gagctccccg
     4501 ttggggaagg gactggagca ggcagcggat cccaggagaa gcccctcgtc caacagggtg
     4561 aaggctccca tgggcgccgg gggcggatcg aaggggaaga ccttgggctt ggagggcagg
     4621 ggggggagct tttccgccag gagggcggtg cgccggaaga gccgggcggg gcagggcgcc
     4681 cagttccgct ggaccgagcg cagggccgcg gcggcctccc ccaaggatgc gaactccagc
     4741 aggaagggcc gagtccagat gttgcgtacc cagcgggcgg gcaggaccgg cccggaccgc
     4801 agcaggaggt ccccgtcctg gcggtcccat tccccgagct ccgcgagcag gtgccgtgtc
     4861 atgcccgggg cggcgtggta gaccgcggcc tcgaggggcc gggcgcccgg ggggatcgcg
     4921 ccaatcacag gagcttgtac ctgcgggccg cctccaccag tcggcggttc cagaggttcc
     4981 gttccaggaa atccgaaccc gcggccgcgt cctcgatccg tccggcccgc tcctgcaggt
     5041 acttgaaagc ccgggacagc acggtagcct tgtccagggg cggttccgcg agccgctcca
     5101 ggagcaggaa gcagaaaaag aggtaggtgg agaaggcggg gtgttcggcc gagagcttgt
     5161 cctcccgggt catgcggtac acctggacgg cccaggcccc ctcccggcgt tccaagcccc
     5221 gggcgaaaca cgcgtaggcg gcccggagat ccgcgtcgaa ggggcggagc gcggaggcgc
     5281 cgaaagccct gcgctcgatg ccgcggtatc cggaatccca gaagagcctc tccgcgggat
     5341 cgaaggtacc gtgctcccag gggggaaggg cctccaggat ctccgcggcc ccgccggggt
     5401 ttccccggag gagttcgagt tccgcccgga agaattccgc ctccccgtcc ccctcgtggc
     5461 gggagagcag ggtccgggcc tcctcgtggc tcccggcgta ggccagggcc cgggcgtccc
     5521 agatgtccgc gcgacgtccg gcctcgtcga atccgtaggc ccgggcgatc gcgcgcaccc
     5581 cctcgaaacc gacccgggct tcctcgtagc ggccgagctc cagatggatc cgggcgcgca
     5641 ggaagacgct ggccgcctcc cagtcccggc ggaagacggc ctgggaggcc gcctcggcct
     5701 ggttcgtcag cttgagggcc ttgtcgagcc gtccggaggc gaacagggcg acggccgtga
     5761 accaccggca gaggagccgc tcgtattcgt cccgggccgc cccggagata tcatgggcgt
     5821 tctcgaaata ctcgatcgac tcctccggcc gctcccgcgc gagggcgcag agccccagga
     5881 ggcgatgaca gcgggcttcc agcttgggct ccggtccacc ccgggcggag agcagggctt
     5941 ccttcgcctg cttcgccgcc tccgggaact ccctccgggc cagggagagc tccgcccgga
     6001 ataggaaggg gaggcccggg ggaagggtcg agcggctgtc cgtctcctcc agttccgcca
     6061 gccgggctga cgcccgggcc tcgtcgccgg agaccaggca ggccgcgagc tcccggagct
     6121 tccgcagggc gggggggatg tccttgtagg cctgccggga ctgatcctgg gggtcctcca
     6181 gggcgtccga cagcacgcag tcgatctcga atcgctgccg gtcctctccg gaaaacgcct
     6241 tcgtggccga caggaggccg agggaggggt aggcctcccg attcctgtaa agcccggtca
     6301 ggtactccgc cagggaagct tcgatccgtt gggcgtccgt ccccacgaag gcggaaagcc
     6361 tgcggttcga catcctcgag gcattccggg gaccgggacc cgagaggtag ccggtcctgc
     6421 ccaggagatc caggacgatc tgacgggcct tggctcggag ccccaggctt tccaggaagt
     6481 cgtggaacct gggggtgtcc aggacatttt cgcagagact ggcggcgtac agcgccgccg
     6541 ccgcctcccg aggcatgagg cccaggatgg cctcggaggg gggcggaccg gacgcgtgct
     6601 cgagttcgag gagggcgcag cggtacagga ggggcaggtc gccccgggcc cgggagaaca
     6661 gggactttcg ggtctcctcg ggggcctccg gcaggacctc ggaaagcttg cccgagtatt
     6721 cctcgaaatc cggggggggc aggtcccatt ccaggatccc gtattcgggc cacgactggg
     6781 gacagttccg ggcggtggtc gccacgatgc gtatgtccga ggacaggagc agctccccca
     6841 ggagatccag gctttccgga gagaaccgat ggaggttctc gcagaccagg atgggcggca
     6901 gaccggcccg ggacatgccc cgggcgtagg cccgcaggac caggcccatg aacaggcggt
     6961 agcccgtctc catggccggg gccagtacgg cccggaaggg gccggcgaaa acgtaccggt
     7021 aggccggcag aaggcgctcg taaagctgac cttcgacgga gccgaggtga acccgggcct
     7081 cggccaggaa atccccggtg atcccgcagg ccaggggatc gaagggccgg gggttgactg
     7141 tcccgccccg gagggtcagg aatcggtcgg gcgggtagcc cagggcctcc agggccccgg
     7201 ctacggtcgc ttccttcccg gtacccgcgg cgcccctcag gatcagcacg ttcttctcgc
     7261 ccccgaccac cagttcgccg atcagctcgg atatgttggc cgcggccttc tcccggcggt
     7321 agaattcctg ggcggtccgg acgagcccgt ccagccggta ggaaggtccc aggacccggg
     7381 ccaggccgga gcccgcggat tccaggttca ggtatccccg ggtttcccgg tccgccgcgg
     7441 gacccagcca ggcatcctcg ggccggtcgg cctccgccca ggctcggtcc atctcggccc
     7501 tcagggtctc ctcgtccggc ccctcgtgca gggccgcgaa gaaatccatg gtgccctcga
     7561 agcggctccg gagcaggtcc cggaaggcaa ggagggcttc gatcgcctgg agccgccaga
     7621 agatggagct cgaatcgaag accgccacga aggggtgttc ccggccggaa gggcgggccc
     7681 cgtagtccgc ggcgatccgc tccatccggg tctgcaggtc cgcagccccc ggggggtcca
     7741 tgcgccgatg ctgcccgtgg tagtcgatgc gtacgtacaa ggcgatcatg cccggccctc
     7801 ccggcaggag tatactccat attgatcctg cgcgcaccga gcgcggtgct tgcccctcgg
     7861 gctcctatcc gatatcttaa tattgtatcc aaaaagtagt acaggaaagc ccggaccccg
     7921 gtcggggatc ccggcgcggt atgccatgac gccgtatctg gaattcaggg aaacgaagag
     7981 cctcgatctg acgttccggt tctgtccagg ccaggagttt cgccaggtgg tcgatgccgt
     8041 tcggtcggtc aaactcccgc ctctcccgct caacgaggag ttggctgcct tctcggtgct
     8101 cgagttgctc tccaattcga tccgggctca ccgggagcgg ggcgtccggg aggacgtgga
     8161 actccggtat tcgatctccg acaaccgcct gaagatagag atcctggact ccggccgggg
     8221 cttcgacccg ggccgcctcc cgtaccggtt ggacagcccc ccggactcga tcgacccccg
     8281 gtcggagacc ttcacggagt accgcctgaa gtacgggaac ggtcgcttcg ggatgggcct
     8341 gctggcggca cggaagacct tccccgcctt ttccctgacc ttcgtcgatc gggacttgag
     8401 tccctgtccc tggtactccg ggatggtccg gggtacccgg atcgtgctgt ccgcccccct
     8461 ggcctcggaa gcctgggcgg aggatctgga ggaactgccc cctgcggagg tggaggaatg
     8521 agccgggaac gccgcgggac ggaccggaac cggggcttcg cccgggccgg tctggtgccg
     8581 tccttgtacg ggtacgtggc ggacgtctcg gctcggggct tccgagccgt tttccgggag
     8641 gacccccgcc tttccaaggg ggcgcccgtc gtgccggtcc tctccttctc cgagatcgac
     8701 gtggaaccct tccccctgcc ctcggagg
//
LOCUS       DAOTEI010000150         8591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5823_length_8591_cov_5.373594, whole genome shotgun sequence.
ACCESSION   DAOTEI010000150 DAOTEI010000000
VERSION     DAOTEI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5823_length_8591_cov_5.373594"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1123
                     /gene="nagA"
                     /locus_tag="P5313_12910"
     CDS             2..1123
                     /gene="nagA"
                     /locus_tag="P5313_12910"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRY81313.1"
                     /translation="MVQNCDLLLEDGRVADVFGERRLAARAFGPEVRVRDASGCYIAP
                     GFTDTHIHGIGGHGTEEGDPREIEAMSRALPRYGVTSFCPTLYPAPRAELVERVRRAA
                     SARTPGDGARILGLHLEGPFISPERLGVQKPESASPVDLDFLEELWEAAGGRILLMTV
                     APELKGMRELALSCAKKGIVLSAGHTNATYENMVEGMQAGILHATHLFNGMSQMHHRN
                     PNAVGAVLIHPEISCEIVADGRHVHPDLVRLVVRDKPLDRIVLVTDSLRPTKQGEGPL
                     FANNQEVEPADGVFRRKSDGVIAGSCLTMIEGVRNLFSWGIPVADAVRMACGNPARVL
                     RRDRLGALVPGYEGDVVVFDREFRVRAAAVRGRLVVDEL"
     gene            complement(1187..2455)
                     /gene="thrC"
                     /locus_tag="P5313_12915"
     CDS             complement(1187..2455)
                     /gene="thrC"
                     /locus_tag="P5313_12915"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRY81314.1"
                     /translation="MRFPSTRGGCEPASASEALRTGLAPDGGLFVPELLPALSPAALD
                     PALPYPERAREVLAPFFEGDELEPDLPELCREAFDFPVPLTRLSDRVRILELFHGPTA
                     AFKDFGACFLARALERLQSGEKTPLTILVATSGDTGGAVAAAFHGRTGISVRVLFPED
                     GVSERQRKQLTCWDGNVASYAVRGTFDDCQRVVKEAFRSPEISRRFRLSSANSINLGR
                     LLPQTVYYVHASALFLAETGEKPALVIPSGNVGNAVGAYWARAMGAPIARIALAVNAN
                     RVLPDFLTEGIYRPRPSVRTLANAMDVGDPSNLERLRFLFPERETLEREVSARSVTDG
                     EIRDTIRRVFEGSGLVLCPHTACAEYVRERRMPEAPSIVVATAHPAKFETVVEPLIGQ
                     AVPVPPALAALLDAPSRHETIEPRLEELLG"
     gene            complement(2452..3426)
                     /locus_tag="P5313_12920"
     CDS             complement(2452..3426)
                     /locus_tag="P5313_12920"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRY81315.1"
                     /translation="MEKKTAKAFAPATVANVAVGFDILGFPLDAVGDTVTVERIDPAP
                     GASPAVTLAGTDGVVSTLPTDPERNTATAPLIRLREDQGLPFGFRVSVRKGIPLSSGM
                     GGSAASSVGALTAANSLLPEPLPRESLLKYALLGEELASGSPHGDNAAPCLLGGLTLL
                     RSLDPVEAVRIPVPEGLRVVLVHPHRELDTRSSRGVLPRDLPLKEYVAQSSRLAAFVA
                     ACYAGNLDLMARSLKDGLIEPRRAPLIPAFHSVQRAALQAGALGCSISGSGPPVFALC
                     RSDDEAEKARRAMAAAWEAAGVPCDSWTGRISQAGARILENGQAGGAA"
     gene            complement(3534..3968)
                     /gene="rpiB"
                     /locus_tag="P5313_12925"
     CDS             complement(3534..3968)
                     /gene="rpiB"
                     /locus_tag="P5313_12925"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRY81316.1"
                     /translation="MRILLANDHGALELKRRISAHLQSRGHEIVDLGVDTEESVDYPD
                     KGAEAAREYLKGGYDLGIVLCGTGIGISIAANKHKGIRCALVHDLFTAEMARAHNDAN
                     FLAFGGRVDYPVPVERILDKFLETKYEAGRHARRVGKLDALG"
     gene            4135..5178
                     /gene="ltaE"
                     /locus_tag="P5313_12930"
     CDS             4135..5178
                     /gene="ltaE"
                     /locus_tag="P5313_12930"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HRY81317.1"
                     /translation="MKYIDLRSDTVTWPTDEMRAAMARAEVGDDVYGDDPTVNELEAL
                     GARLTGKEAALFVPTGTFGNQLALFTHCPRGTEVILGDDCHIVAHEAGAASVIAGVQL
                     RTLVSREGELDPAEVESRIRPGGDIHYPDTSLVCLENAHGCGRALSLSYMDAVRAVAD
                     RHGLPIHLDGARLFNAAAALGVEAREIAARVDSVMFCLSKGLCAPVGSLLAGTRAFIE
                     AARRKRKLMGGGMRQAGVLAAAGLLALGPMRERLSEDHRNAAALAEGLARIPGVRILG
                     SGVRINMVFLQIEGLSDAGAFSAALERRGILVNPPEGGALRLVTHYWIKAERIPVIVS
                     AVAEAAAEAGGRA"
     gene            5175..6104
                     /locus_tag="P5313_12935"
     CDS             5175..6104
                     /locus_tag="P5313_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRY81318.1"
                     /translation="MKRETLGVLLCYALVYVVWGTTYYFIRAAVHTMPASLVVALRFL
                     AGAVLLAGFALAKGDFRKGLSWRQAGGSALLGILLLLLGNGLLTIAEKTIPSYIASLI
                     VVGGPFYIAAFNYLLFRTRISAIRIGGVLIGVAGIAVLLYDGTSLAGTFHPAVLIAVS
                     GSLCWAFGTSISRALPKAESVFLSTAIQMAVAGGVSLVSALASVPDLGAAFRGVSPWS
                     WFSVGYLAFMGTVTLAAYNHLLVKEPSFRISSYALVNPLIAVALGLAVGEKATPFLAF
                     GVPLILAGLTLMLYGDPIRERLSRKKPAPDASV"
     gene            6142..7221
                     /locus_tag="P5313_12940"
     CDS             6142..7221
                     /locus_tag="P5313_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81319.1"
                     /translation="MNSPFGAPSGVPAAARSGAAAAAAGCILIAAVLLSACLPQVVSE
                     AFSPRDLRPPSVRVWDAPGPGEFLVDFDEDVEADPGDFAADPDLGGLEVSSEGSRVRI
                     TASGLPPPGSALSLEGTVRDAEGNSTSFVLPFWAYNPALPRVLINEVLTQGSSTHPDL
                     VELAVLESGNLAGLAFRVGCAVKQILVYVFPPCEVAAGEYAILHLKPQGLPEEVDETG
                     DTSSSGGQDAYPYSRDFWYRGGDGALPGENGCVTLYRSPTGPVLDALLYSARTSASDT
                     KYGGFGTQALLDQARAVVAEGMWRIAGSEVRPEDAARSEGSTSTRTLCRSSGSGDTDS
                     ASDWHVVPTKGSTVGKPNSDEVYVP"
     gene            complement(7374..7697)
                     /gene="trxA"
                     /locus_tag="P5313_12945"
     CDS             complement(7374..7697)
                     /gene="trxA"
                     /locus_tag="P5313_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRY81320.1"
                     /translation="MSHEVTLTSDNFEEEVLKSPIPVLVDFWAEWCGPCRMIAPAVEE
                     LARTYEGRIKVGKVDVDSQGELAGRFGIISIPTLMVFKDGKAARQKVGALPKPQIEGL
                     FKDLV"
     gene            7855..8475
                     /locus_tag="P5313_12950"
     CDS             7855..8475
                     /locus_tag="P5313_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRY81321.1"
                     /translation="MLNTFLQIFLTVFIVIDPIGIVPLFISATNRMTEAQKKRTIWKA
                     VAVAFAVLGIFTLAGRFILSFFGISPGSFYVAGGTLFFLISIDMLFGQPKRTKTSKEE
                     EEEDSSSVAVFPLAIPMIAGPGTITTLMLYSADAPSYLTITLLLFGAIVLSLAGVTLA
                     MFSSRIVLRILGRTGVSVVERMMGLVLSGLAIQFVYDGLVKLGILG"
BASE COUNT         1228 a         2890 c         3033 g         1440 t
ORIGIN      
        1 cctggtacag aactgcgacc ttctgctgga ggacggacgg gtggcggacg tcttcgggga
       61 gcgtcgcctg gccgcccggg ctttcggccc ggaggtccgg gtccgggacg cctcgggctg
      121 ctatatcgcc ccgggtttca cggataccca tatccacggc atcggggggc acggcaccga
      181 ggagggggat ccccgggaga tcgaggcgat gtcccgggcc ctgccccggt acggggtgac
      241 ctccttctgc ccgaccctgt accccgcacc ccgggccgaa ctcgtagagc gggtccgccg
      301 ggcggcctcg gcccggactc ccggggacgg ggcgcggatc ctggggctcc acctggaggg
      361 ccccttcatc tcccccgagc gtctgggtgt ccagaagccc gagtcggcca gcccggtcga
      421 cctggacttc ctggaggaac tctgggaggc cgcggggggc cgcatccttc tcatgaccgt
      481 ggccccggag ctcaagggca tgcgggaact ggccctgtcc tgcgccaaga agggcatcgt
      541 actttccgcg gggcacacga acgccaccta cgaaaacatg gtggagggga tgcaggcggg
      601 gatcctccac gcgacccacc tgttcaacgg catgagccag atgcaccacc ggaaccccaa
      661 cgccgtcggg gcggtcctga tccatccgga gatctcctgc gagatcgtgg cggacggccg
      721 tcacgtgcat ccggacctgg tccgcctggt ggtccgggac aagcccctgg accgcatcgt
      781 cctggtcacg gactccctgc ggcccacgaa acagggggag gggcccctgt tcgcgaacaa
      841 ccaggaggtc gagccggcgg acggggtctt ccgccggaaa tcggacgggg tcatcgcggg
      901 ctcctgcctg accatgatcg agggggtccg gaacttgttc tcctggggga ttcctgtggc
      961 ggatgcggtg cgcatggcct gcggaaaccc cgcccgggtc ctgcgccggg accgcctcgg
     1021 ggccctggtg cccgggtacg agggggacgt ggtcgtcttc gaccgggagt tccgggtccg
     1081 cgctgccgcg gtccgggggc ggctcgtggt ggacgagctc taggagcctg tcggacttgt
     1141 ggatatttta cgccgatagg cgctaaagat cccgattatg cggctcctac ccgagcagtt
     1201 cctcgagcct cggctcgatg gtctcgtgcc ggctcggggc gtccaggagg gccgccaggg
     1261 cggggggcac cgggacggcc tgcccgatca ggggctccac gacggtctcg aatttggccg
     1321 gatgcgcggt ggccacgacg atggaggggg cttccggcat gcggcgctca cggacgtatt
     1381 ccgcgcaggc ggtgtgggga cagagcacga ggccggaacc ctcgaacacc cgccggatgg
     1441 tatcccggat ctccccgtcc gtcacgctcc gggcggaaac ctcccgctcc agggtctccc
     1501 gctcggggaa gaggaagcgc aggcgttcca ggttactcgg gtcccccacg tccatggcgt
     1561 tggccagagt ccggacgctg gggcggggcc ggtagatccc ctcggtcagg aaatccggca
     1621 ggacgcggtt ggcgttgacg gccagggcga tccgtgcgat cggggccccc atggcccggg
     1681 cccagtaggc gcccacggcg ttgcccacgt tcccgctggg aatcacgagc gcgggtttct
     1741 ccccggtctc ggccaggaag agggccgagg cgtgcacgta gtagacggtc tggggcaaaa
     1801 ggcggcccag gttgatgctg ttggccgagg ataggcggaa gcggcgggag atctccgggg
     1861 agcggaaggc ttccttgacc acccgctgac agtcgtcgaa ggttccccgc acggcgtagg
     1921 aggcgacgtt gccgtcccag caggtaagct gtttcctctg gcgttccgaa acgccgtctt
     1981 ccgggaagag gacccggacg gagatcccgg tccggccgtg gaaggccgcg gcgacggccc
     2041 ccccggtgtc cccggaggtg gccacgagga tggtcagggg agttttctcc ccggactgga
     2101 ggcgctccag ggcgcgggcc aggaagcagg ccccgaagtc cttgaaggcc gcagtggggc
     2161 cgtggaagag ctccaggatc cgcacccggt ccgagagccg ggtcaggggc acggggaaat
     2221 cgaaggcctc ccggcagagc tccggcaggt ccggttccag ctcgtcgcct tcgaagaagg
     2281 gggccaggac ttcccgggcc cgttcggggt aggggagggc cgggtccagg gccgcggggg
     2341 acagagccgg taggagttcg gggacgaaga ggcccccgtc cggggccagc cccgtccgca
     2401 gggcctcgga ggcggaggcc ggctcgcagc ctccccgggt gctcgggaac ctcacgcggc
     2461 ccctccggct tggccgtttt ccaggatccg ggcgcctgcc tggctgatcc ggcccgtcca
     2521 ggagtcgcag gggacccccg ccgcctccca ggcggccgcc atggcccggc gggccttctc
     2581 ggcctcgtcg tcgctccggc agagggcgaa gaccgggggg ccggatccgg agatggagca
     2641 gcccagggct cccgcctgga gggcggccct ctggacggag tggaaggccg ggatcagggg
     2701 ggcccgccgg ggctcgatga gcccgtcctt gaggctccgg gccatgaggt ccaggttccc
     2761 ggcgtagcag gcggcaacga aggcggccag ccgggaggac tgggctacgt attccttgag
     2821 gggaaggtcc cggggcagga cgccccggct ggaccgggtg tccagctccc ggtgggggtg
     2881 gaccaggact acccggagcc cttccgggac ggggatgcgg accgcctcca cggggtccag
     2941 ggaccgcagg agcgtgagcc ctcccaaaag gcagggggcc gcgttgtccc cgtgggggga
     3001 tcccgaggcc agttcctccc ccaggagggc gtacttgagc aggctctccc ggggcagggg
     3061 ctcgggcagg agggagttgg ccgccgtcag ggcgcccacg gagctggccg cggagcctcc
     3121 catcccgctg gacaggggga tgcccttgcg cacggagacc cggaacccga aggggaggcc
     3181 ctggtcctcc cggaggcgga tgaggggcgc cgtcgcggtg ttgcgctcgg gatccgtcgg
     3241 cagggtcgac acgaccccgt ccgtcccggc cagggtgacc gccggggagg cgcccggggc
     3301 cggatcgatt ctttcgacgg taaccgtgtc ccccacggcg tccaggggaa agcccaggat
     3361 gtcgaagccc acggccacgt tggccacagt cgccggggcg aaggcttttg ccgttttctt
     3421 ttccatgttc agctcccgtt gcggggattc cggcggatac tgcgtccgcc ggctggtacg
     3481 aagcttgtga aggttcccgt gcggcgccag gccggggacg tgcccggagc ggttcagccc
     3541 agggcgtcca gctttccgac ccgacgggcg tggcgcccgg cctcgtactt cgtctccagg
     3601 aatttgtcca atatccgctc caccggaacg gggtagtcca cccggcctcc gaaggccagg
     3661 aagttggcgt cgttgtgggc ccgggccatc tccgcggtga agaggtcgtg gaccagggcg
     3721 cagcggatgc ccttgtgctt gttggcggcg atggagatcc cgatcccggt gccgcacagg
     3781 acgatcccca ggtcgtagcc gcccttgagg tactcccggg cggcctcggc gcccttgtcg
     3841 gggtagtcca cgctctcctc ggtgtccacc ccgaggtcca cgatctcgtg tccccggctt
     3901 tgcaggtggg cggagatcct ccgcttgagc tccagggcgc cgtggtcgtt ggccagcagt
     3961 atccgcatgg tgtccgctcc cgtcccggcc ggtttagcag gctggttcag cccgcaaggt
     4021 cgctccaccg gccgaatttc tgcggcggag tataccattt cagcgcggga aggtaagggg
     4081 ccgaccgcgc cccgccgctt gccccgcccg cccccggcgc gtactatgtc cggcatgaag
     4141 tacatcgacc tgcggagcga tacggtcaca tggccgaccg acgagatgcg ggccgccatg
     4201 gcccgggcgg aggtggggga cgacgtctac ggggacgacc ccacggtcaa cgagctggag
     4261 gccctggggg cccggctaac gggcaaggag gcggccctct tcgtgccgac cgggaccttc
     4321 gggaaccagc tggccctctt cacccactgc ccccggggca ccgaggtgat cctgggggac
     4381 gactgccata tcgtggccca cgaggcgggg gcggcctcgg tcatcgcggg cgtccagctc
     4441 cggaccctcg tatcccggga gggcgagctg gacccggcgg aggtcgagtc ccggatccgg
     4501 ccgggcgggg acatccacta cccggacacc tccctcgtct gcctggagaa cgcccacggc
     4561 tgcggccggg ctctgtccct ctcctacatg gacgcggtcc gggccgtcgc ggaccggcac
     4621 ggcctgccga tccacctgga cggggcgcgg ctgttcaacg cggcggccgc cctgggggtc
     4681 gaggcccgcg agatcgccgc ccgggtcgac tctgtgatgt tctgcctttc caaggggctg
     4741 tgcgcccccg tgggatccct cctggcgggt actagggcct tcatcgaggc cgcccgccgc
     4801 aagcgcaagc tcatgggcgg cgggatgcgc caggcgggcg tcctggccgc cgcgggcctc
     4861 ctggccctgg gccccatgcg ggagcgcctt tccgaggacc atcggaacgc cgccgccctg
     4921 gccgagggcc tggcccggat tccgggggtg cggatcctgg ggtccggggt ccggatcaac
     4981 atggtcttcc tccagatcga ggggctttcc gacgccgggg ccttctccgc ggccctcgag
     5041 cgccggggca tcctggtgaa tccccccgag ggaggggccc tccgtctggt gacccactac
     5101 tggatcaagg cggagcggat cccggtcatc gtctccgcgg tcgcggaggc ggcggccgag
     5161 gcgggcggcc gggcatgaag cgggaaaccc tcggggtcct cctctgctac gccctggtgt
     5221 acgtggtgtg ggggacgacc tactacttca tccgggcggc ggtccatacc atgcccgcat
     5281 ctctggtggt ggccctccgc ttcctggccg gagccgtcct cctggcgggg ttcgccctgg
     5341 ccaaggggga cttccggaag ggcctttcct ggagacaggc gggaggttcc gccctcctgg
     5401 ggattctcct tttgctcctg gggaacggcc ttttgaccat cgcggaaaag accataccca
     5461 gctacatcgc cagcctcatc gtcgtggggg gacccttcta catcgcggcc ttcaactacc
     5521 tcctgttccg tacccggatc tccgcgatcc gcatcggggg ggtgctgatc ggggtcgcgg
     5581 ggatcgcggt gctcctgtac gacggaacct ccctggccgg gaccttccac cccgcggtcc
     5641 tgatcgccgt gtccggttcc ctctgttggg ccttcggcac cagcatctcc cgggccctgc
     5701 ccaaggcgga gagcgtcttt ctgtccacgg ccatccagat ggccgtggcc gggggggtct
     5761 ccctcgtctc ggccctcgcg tccgtcccgg acctcggggc cgccttccgg ggggtctcgc
     5821 cctggtcctg gttctccgtg gggtacctgg ccttcatggg aaccgtcacc ctggcggcct
     5881 acaaccacct cctggtcaag gagccgagct tccggatctc cagctacgcc ctggtgaatc
     5941 ccctgatcgc cgtggccctg ggcctggcgg tcggggaaaa ggcgacgccc ttccttgcct
     6001 tcggggtccc cctgatcctg gcgggtctga ccctcatgct ctacggcgac cccatccggg
     6061 agcgcctttc ccgaaaaaaa cccgccccgg acgcaagcgt atagaccggt tcctcccgga
     6121 ccatccccgg gaggaacgag tatgaatagt ccgttcgggg cgccgtccgg cgtcccggca
     6181 gccgcccgga gcggcgccgc ggccgcagcc gcgggatgta tcctgattgc cgccgtcctg
     6241 ctctccgcct gcctgcccca ggtcgtgtcg gaggcgtttt caccccggga tctccgtccc
     6301 ccgagcgtac gggtctggga cgccccgggt ccgggggagt tcctggtgga cttcgacgag
     6361 gacgtggagg cggatccggg ggatttcgcc gcggatccgg acctgggcgg cttggaagtc
     6421 tcgtccgagg ggagccgggt gaggatcacg gcctccggac tcccgccccc cggttcggcc
     6481 ctgtccctgg aagggaccgt acgggacgcc gagggcaatt cgaccagctt cgtgcttccc
     6541 ttctgggcgt acaatccggc gctgccccgg gtcctgatca acgaggtcct gacccaggga
     6601 tcctcgaccc atccggacct ggtggaactg gcggtgctgg aatccggtaa tttggcgggg
     6661 ctcgccttcc gggtaggctg cgccgtcaag cagatcctgg tctatgtctt cccgccctgc
     6721 gaggtcgcgg ccggggagta cgcgatcctg cacctcaagc cccagggcct gccggaggaa
     6781 gtggatgaga ccggggatac ttcatcttcc ggaggtcagg acgcctaccc gtattcccgg
     6841 gacttctggt accggggagg cgacggggcc ctgccggggg aaaacgggtg cgtgaccctg
     6901 taccgttccc ccacggggcc ggtcctggac gccctgctct actccgcccg gacctcggcg
     6961 tcggacacga agtacggggg cttcgggacg caagccctcc tggaccaggc ccgggcggtc
     7021 gtcgcggaag ggatgtggcg gatcgcggga tccgaggtcc ggccggagga cgccgcccgg
     7081 agcgaaggaa gcaccagcac gcgcactcta tgccgatcct cgggctcagg agacacggac
     7141 tcggcctcgg actggcacgt cgttccgacc aagggctcga ctgtgggcaa acccaactcg
     7201 gacgaggtct acgttccctg acttcggggc tgggagtttg tcgggcttgc ccaccttgaa
     7261 tagtagaaaa atccctttta tgcgattttt ctactttgcg aactccaaag gagccggaaa
     7321 atcgggattt ttagcgtcta tgggcgctat aaatctacaa gtccgacagg ctcctagacc
     7381 aggtccttga agagcccctc gatctggggc ttgggcaggg cgccgacctt ttggcgcgcg
     7441 gctttgccgt ccttgaagac catgagggtg gggatggaaa tgatgccgaa gcggccggcc
     7501 agctctcctt gggaatccac gtccaccttg ccgaccttga tcctgccttc gtaggtccgg
     7561 gcgagctcct ccacggccgg ggcgatcatc cggcaaggac cgcaccactc cgcccagaaa
     7621 tccaccagca cggggatggg ggacttgagg acttcctctt cgaaattgtc ggacgtaagg
     7681 gtcacttcgt ggctcatctt ccctcccggc ggcggataaa attcggttgt tcggcggccg
     7741 gccgtgaacg ggccggaacg atacgggaaa tatagggaga aacggcggcg gagtcaaatg
     7801 cggggcggac ccgccggcct tgcgctcccg gttcccgcgc tgtatccttc cgccatgctg
     7861 aataccttcc tgcagatctt cctcaccgtc ttcatcgtga tcgatcccat cggcatcgtg
     7921 cccctgttca tctccgcgac gaaccgaatg accgaggccc agaagaagcg gaccatctgg
     7981 aaggccgtcg cggtggcctt cgcggtcctg gggatcttca ccctggcggg gcgcttcatc
     8041 ctctccttct tcgggatttc cccgggctct ttctacgtgg ccggcggaac cctcttcttc
     8101 ctgatctcca tcgacatgct cttcggacag cccaagcgca ccaagacgag caaggaggag
     8161 gaagaggagg actcatcctc ggtggcggtc tttcccctgg ccatccccat gatcgcgggt
     8221 cccgggacca tcacgaccct catgctctat tccgcggacg cgccttccta cctgacgatc
     8281 accctcctgc tcttcggggc catcgtcctc tccctggcgg gggtgaccct ggccatgttc
     8341 tcctcccgga tcgtcttgag aatactgggc cggacggggg tttccgtggt ggagcggatg
     8401 atgggcctcg tgctctcggg cctggcgatc cagttcgtgt acgacgggct cgtgaagctg
     8461 ggcatcctgg gctgatccgc cgtcggtgga cgaaaaaagc cgaggcgggc acaccgcctc
     8521 ggctttttca tatcggtccg cgggtcaggc cgtcttcggg accggcgcct tcaggaactt
     8581 ctggatcagg a
//
LOCUS       DAOTEI010000151         8537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5869_length_8537_cov_5.698892, whole genome shotgun sequence.
ACCESSION   DAOTEI010000151 DAOTEI010000000
VERSION     DAOTEI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5869_length_8537_cov_5.698892"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="P5313_12955"
     CDS             complement(<1..391)
                     /locus_tag="P5313_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY81322.1"
                     /translation="MKRRVFVLVIAIFLIAAAALSAQDKTYTIRFNTVASPSDIQVEA
                     MNKFAEVATALSGGKLDVKVFHSGQLGDQKTALLGVMKGSLEMACDASPAWLADIGSY
                     PEIGVLEVAYLFRDIDHMYRVLNGPVGQ"
     gene            complement(448..1224)
                     /locus_tag="P5313_12960"
     CDS             complement(448..1224)
                     /locus_tag="P5313_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRY81323.1"
                     /translation="MKQVRSIQRALEIVDSFRLGQSKGVSEISREMGIPKSTAHEILA
                     TLVEAGVLARDDVTGLFQLGTKLIELGNRARSNYPLNRAAEPFIKALRDEFDETVFLT
                     LLERDQVFYLDCYESTRRLRIFSTLGDRAPLYCTSLGKAILAAMPPDELDRYLSSVKL
                     EPFTVNTITDPVLLREDIERTARRGYAVDDMEHEEGVRCLGAAIRNEAGRPIGAVSVS
                     GAAGRISGEREAEIAVRVVAAAEGTSRAMGYRPGESRQNQ"
     gene            complement(1371..3191)
                     /locus_tag="P5313_12965"
     CDS             complement(1371..3191)
                     /locus_tag="P5313_12965"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HRY81324.1"
                     /translation="MANLESLKPRPAARRFPGPAPKIGIRPAIDGRLGGVRESLEDVT
                     MGMARAAARLISDNLRHPDGTPVECVLADSTIGGVAEAAACAEKFSREGIQVTLTVSP
                     CWCYGSETMDMDPLTVKAVWGFNGTDRPGAVYLAAVLAAHNQKGLPAFGIYGRDVQDL
                     KDTGSVPPDVRDKILRFARAGLAVAWMRGKSYLSIGSVSMGIAGSIVDPDFFQSWLGM
                     RTEYVDMSELVRRFEEKIYSEAEYRRALAWVRENCPEGSDVNPPSIQHSRARKDKAWE
                     TSVKMALIVRDLMVGNPELRRKGRGEEALGHNAILAGFQGQRAWTDHFPNGDFLETIL
                     NSSFDWTGIRQPYLVATENDSLNGAVMLFGHLLTGTAQIFSDVRTFWSPEAVERVTGW
                     KPEGASAGGFIHLINSGATTLDGTGRQRRDGKPAMKPWWEITAAEAGACLDATSWRYA
                     DLGYFRGGGYSSDFLTEGGMPVTMTRLNLAAGAGPVLQIAEGVTATLPQQVHDKLDRR
                     TNETWPTTWFVPRLTGRGPFRDVYSVMAAWGANHGAVCYGHVGADFAALAAALRIPVC
                     MHNLEESSLFRPSYWNSFGMDPEGSDYRACSALGPLYGRR"
     gene            complement(3224..4216)
                     /locus_tag="P5313_12970"
     CDS             complement(3224..4216)
                     /locus_tag="P5313_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY81325.1"
                     /translation="MSRAATVRDIAREAGVSTATVSRALNGDPRVHGTTASAVRAAAD
                     RLGYSVNRAARSLKTRSTRMVGVIAPDLATDFFMILADRLERELAAEGYDMLIASSRE
                     SAAEEERLLGLFPEHLVDGIVAIPATGESDHFRRAIARGTPLVLTDRAPGNPGADAVL
                     VDNEGGAREAVRALAGLGYRRIGFVGGDLRLTTARERFEGYRTALAGLGIPPEPEFER
                     FGPMHIDAGYGMMDELLALPNAPEAWFVVNADTHVGATNRLYKEPPERRERAALAAFD
                     EMPWSPLLRFCRFAVEQPVGEMGRTAAGFILDRIEGRVGGRPRTARLPTRLIPH"
     gene            complement(4213..4665)
                     /gene="fucU"
                     /locus_tag="P5313_12975"
     CDS             complement(4213..4665)
                     /gene="fucU"
                     /locus_tag="P5313_12975"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HRY81326.1"
                     /translation="MLIGIAPCITPELLAVLHRMGHGDEIVLADAFFPGESCNPRTLR
                     ADGIRVAELLDGVLALINPDGHDPAPFLMMQPVPGDPLDPGVEREYRTVIDRRWPASP
                     PIARLERFAFYERARRAFAVVMTGETAPYGNLIVKKGVIPAAPGGRPV"
     gene            5111..6016
                     /locus_tag="P5313_12980"
     CDS             5111..6016
                     /locus_tag="P5313_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81327.1"
                     /translation="MRVASCRPRLVAFAAVSILALAARTASAQSPLSFDVSLAPNTSG
                     TLEGSASLRLRWASFGESGLTFSASSFLEEYPEGGGTTTVIQSRKTLDLEVFRLPSDI
                     LALRFGRTGGVSLSPVALVNGVMLDEDKYGEDTGVVYYVGQRTIWAKPLLGADLKLSL
                     GTASVEVYYRTSWPFTLREYLEGIANYSTVVTPFSVWDEGFETRLGGTLSFRPAPGWT
                     VRGGVDWVRHIGYSVAVAGGVATDFVYESQSLEGSLTLTFPVGERRPMVGVAYAYDAF
                     LPVDAIYDQKSYTNGRIRFLLGLEL"
     gene            complement(6066..6863)
                     /locus_tag="P5313_12985"
     CDS             complement(6066..6863)
                     /locus_tag="P5313_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY81328.1"
                     /translation="MEFGGKTVWITGASSGIGKALALEFFRRGAVVVLSARSADKLGE
                     LKEILDGIETGRCHVVPCDVTDPESVLRAAGAVKSAIGRLDILVNNAGVSQRSTALET
                     ESRVERALFEVNFFGAVAVTRNALPWMIETGGGRIVVIGSMAGKFGFPMRSTYCASKH
                     ALQGYFETLRAELRGSGVGVTLVCPGRIRTDISIHSLKGDGTVYGRMDAGQERGMPVE
                     RCARIIVEAVRRRRKEILIGAPERLLYFIRRFCPPLFYGITERINPI"
     gene            7143..8354
                     /locus_tag="P5313_12990"
     CDS             7143..8354
                     /locus_tag="P5313_12990"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRY81329.1"
                     /translation="MSEYRSNRLRTFRIRPGRSSPGPRISETFSLALLAAALILVPPP
                     VAAQAGGGAAGVFRSREHTFRLVKVLDRLDYPWALAFLPDGSMLISERTGRLLRVREG
                     RAQPVSGVPTVNASGQGGLLDLAVSPSFASDRTVFFTYSQPGAGGAGTALARAVLDGT
                     SLREVRTIWSMERKTGAGQHFGSRIRLLQDGTILLTTGDRGQGSRAQDLGDDAGKVHR
                     VAPDGSVPADNPFRGNRAARPSVFSYGHRNPQGLAIHPGTGEAWITEHGPQGGDEVNL
                     VRPGRNYGWPVITFGRQYGSGAQIGEGTAKPGMEQPLIHWTPSIAPSGLEFYTGDAFP
                     RWKGSLFSGNLAGRRLVRMTVGDAQVLSQEVLLEGSVGRIRDVRQGPDGFLYILTDES
                     PGAVYRLEPGD"
BASE COUNT         1350 a         2851 c         2840 g         1496 t
ORIGIN      
        1 tctgcccgac cggtccgttc agcacccggt acatgtggtc gatgtcccgg aagaggtagg
       61 ccacctcgag gacgccgatc tcgggataac ttccgatgtc cgccagccaa gccgggctcg
      121 cgtcgcaggc catctcgagg gatcctttca tgaccccgag cagggcggtc ttctggtccc
      181 cgagctgacc ggagtggaag accttgacgt ccagcttgcc gccgctcagg gccgtcgcca
      241 cctcggcgaa cttgttcatc gcctccacct ggatgtcgct cgggctagct accgtgttga
      301 aacggatggt gtaggtcttg tcctgggcgg acagggccgc ggcggcgatc aggaagatcg
      361 cgatcaccag aacgaaaact cgtcttttca tgatacaagc acctcctgaa aatgaccttt
      421 cggcggacat cggcccgccg cggaaaccta ttgattttgc cggctctcgc cgggccggta
      481 ccccatcgcc ctggatgtcc cctccgccgc tgccacgacc cggacggcga tctccgcctc
      541 ccgttccccg gatatccggc cggctgcgcc ggatacgctc acggccccta tcggtcggcc
      601 tgcctcgttg cggattgccg cgcccaggca gcgcaccccc tcctcgtgct ccatgtcgtc
      661 caccgcgtag cccctccggg ccgttcgctc gatgtcctcc cggaggagga cagggtccgt
      721 gatggtgttc accgtgaagg gctcaagctt gacggaggac aggtatcgat ccagctcgtc
      781 cgggggcata gccgcgagga tggcctttcc cagggaagtg cagtacaggg gagcccggtc
      841 tccgagggtc gagaagatcc ggagccgccg ggtggattcg tagcagtcga gatagaagac
      901 ctggtcccgt tcgagcagcg tgaggaagac cgtctcgtcg aattcgtccc gaagggcctt
      961 gatgaagggt tcggcggcgc ggtttagggg gtagttcgag cgggcgcggt tcccgagctc
     1021 gatgagcttg gtccccagct ggaataggcc ggttacgtcg tcccgggcca ggacgcccgc
     1081 ctcgacgagg gtggccagga tctcgtgggc cgtgctcttg ggtatcccca tctcgcggct
     1141 gatctcggag actcccttgc tctggccgag ccggaaggag tcgacgatct cgagggcccg
     1201 ctggatggat cggacctgtt tcataacgcc tcggagttgt ccgtatatac ggacgtctgc
     1261 cgtgtatgtg aacagtttag acccgtactc cccgacttgt caaatcattt cgaccgcagc
     1321 cgggatgatc acggccgcgg ttccgaccgc cgcggccgtt ccgggggact tcacctcctg
     1381 ccgtagagag gtcccagggc ggagcaggcc cggtagtccg agccttccgg atccatcccg
     1441 aaggagttcc agtagctcgg ccggaagagg gacgactcct cgaggttgtg catgcagacc
     1501 gggatgcgca gggcggccgc cagggccgcg aagtccgccc cgacgtggcc gtagcacacg
     1561 gccccgtggt tggctcccca tgcggccatg accgaataga cgtcccggaa ggggcccctc
     1621 cccgtcaggc gcggcacgaa ccaggtggtg ggccaggttt cgttggttcg cctgtccaac
     1681 ttgtcgtgca cctgctgagg cagggtggcc gtgacgccct ccgcgatctg aagcacgggc
     1741 cccgccccgg ccgccaggtt cagtcgggtc atcgtcacgg gcatgccgcc ctcggtgagg
     1801 aagtccgagg agtagcctcc tccacggaag tagcccaggt cggcgtacct ccagcttgtc
     1861 gcgtcaaggc aggcgccggc ctccgccgcc gtgatttccc accagggttt catagccggc
     1921 ttgccgtccc ggcgctgcct gcccgtgccg tccagggtgg tcgccccgga attgatgaga
     1981 tggatgaaac cgcccgccga ggctccttcg ggcttccagc ccgtgacgcg ctccacggcc
     2041 tcgggactcc agaaagtgcg cacgtcggag aatatctggg ccgtcccggt gagcaggtgc
     2101 ccgaagagca tgaccgcccc gttcagggag tcgttttccg tggcaaccag gtagggctgt
     2161 cgtatcccgg tccagtcgaa gctggaattg aggatggttt ccaggaagtc cccgttgggg
     2221 aagtggtccg tccaggcgcg ctgtccctgg aagcccgcca ggatggcgtt gtgccccagg
     2281 gcttcctccc cccggccttt cctccggagt tcgggattcc cgaccatgag gtcccggacg
     2341 atcagggcca tcttgacgct ggtctcccag gccttgtcct tgcgggcccg ggaatgctgt
     2401 atcgaaggcg gattgacgtc ggatccctcc gggcagtttt cacggaccca ggcgagggcg
     2461 cggcgatatt cggcctcgga atatatcttc tcctcgaagc ggcgcacgag ttcgctcatg
     2521 tccacgtact cggtccgcat gcccagccag gactggaaga agtccggatc gacgatggaa
     2581 ccggctatcc ccatggagac ggacccgatg gacaggtatg actttccccg catccaggcg
     2641 acggcaaggc cggctcgggc gaagcgcagg atcttgtccc gcacgtccgg cgggacggaa
     2701 cccgtgtcct tgagatcctg gacgtcccgg ccgtagattc cgaaggcggg caggcccttc
     2761 tggttgtgag ccgccaggac ggccgccagg tagacggccc cgggacggtc ggtgccgttg
     2821 aagccccaga ccgccttgac ggtcagggga tccatgtcca tggtttccga cccgtagcac
     2881 cagcaggggc tcacggtgag agtgacctgg atcccctccc gcgagaattt ctccgcgcag
     2941 gccgcggcct ccgcgacgcc tcctatggtc gagtccgcca ggacgcactc gaccggggtg
     3001 ccgtcggggt gcctcaggtt gtccgagatc aaccgggccg cggcccgggc catgcccatg
     3061 gtcacgtcct ccagggattc ccggactccc ccgaggcgcc cgtcgatcgc gggccggata
     3121 ccgatcttgg gggccggtcc gggaaacctc cgcgccgccg gcctgggctt cagactttca
     3181 agattcgcca tgtcctgctc cttcgccgct ccgggcggcc ctttcaatgc gggatgagcc
     3241 gggtgggcag gcgcgccgta cggggccgcc ctccgaccct gccttcgatt cgatccagga
     3301 tgaagcccgc cgccgtcctg cccatctccc ccaccggctg ttccaccgcg aaccggcaga
     3361 accggaggag gggcgaccag ggcatctcgt cgaaggcggc caaggcggcg cgttccctac
     3421 gctccggcgg ttccttgtac agccggttgg tcgccccgac atgggtgtcc gcgttcacca
     3481 cgaaccaggc ctccggtgcg ttcggcaggg ccagtagttc gtccatcatg ccgtagcccg
     3541 cgtcgatgtg catgggaccg aaccgctcga actccggttc gggggggatg ccgagaccgg
     3601 ccagggccgt acggtaaccc tcgaagcgtt cccgggccgt ggtcagacgc aggtctccgc
     3661 ccacgaatcc gattctgcgg tagccgagcc ccgccagcgc ccggaccgcc tcccgggccc
     3721 ccccctcgtt gtcgacgagt acggcgtccg cgcccgggtt ccccggagcc ctgtccgtca
     3781 gcactagggg cgtgccccgg gcgatcgcgc gccggaagtg atccgattcc ccggtggccg
     3841 ggatggcgac gatgccgtcc acgaggtgct ccgggaacag gcccagcagg cgctcctcct
     3901 ccgccgcgct ctccctcgag gaggcgatca acatgtcgta accctcggcg gcgagctccc
     3961 gttcgagacg gtccgcgagg atcatgaaga aatccgtagc caggtccggc gctatcaccc
     4021 cgaccatcct ggtcgaccgg gtcttcaggc tgcgagccgc ccggtttacc gagtacccca
     4081 ggcggtctgc cgcggcccgc accgccgagg cggtcgttcc gtggacgcgg ggatccccgt
     4141 tcagggcgcg ggacaccgtg gccgtggaaa ccccggcttc ccgggctatg tcccggaccg
     4201 tggccgcgcg actcacacgg gacgtcctcc gggagccgcc ggtatcacgc cttttttcac
     4261 gatgaggttg ccgtacgggg ccgtttcgcc ggtcatgact accgcgaagg cccgccgcgc
     4321 ccgctcgtag aaggcgaagc gctctagacg ggcgatcggg ggagacgcgg gccagcgtcg
     4381 gtcgattacg gttcggtact cccgctccac cccggggtcc aaggggtccc cgggaacggg
     4441 ctgcatcatg aggaaggggg cgggatcgtg gccgtcgggg ttgatgaggg ccaggacgcc
     4501 gtccaggagt tccgcgacgc ggatcccgtc ggcccggagg gtccggggat tgcagctttc
     4561 cccggggaaa aaggcgtccg ccaggacgat ctcgtccccg tgacccatgc ggtggaggac
     4621 cgcgaggagt tcgggagtga tgcagggcgc gattccaatg agcatggcga tcccccggcg
     4681 gcgtaaaaca ggcgccgtct aaggatgtaa tcgtttaccc ggttggcagg cttgtcaaga
     4741 ggaaaatgta atcgattaca aaacccgcac cgtcgaaggc gcctcctccg agagtggtga
     4801 tgctcgtcct aaaggaaacg acacccggtc ggaacggctc tatgatgttc ccctctcgga
     4861 tccgggaagt gccatcgaac cgttccgcgg gtcacttcga agggacctgc gtattccggg
     4921 gaggaggcgc aggttcgccg ccgtccgtgg tgtcgtgtca atcgacgtcg ggatcgggtg
     4981 ctcagcctcg gttgacgtgt gttttcaggg aagtatacta cagtacgtaa gaatttcatc
     5041 tgtaaaaacg cgacgaggtt gcgggaacga tttgttcgga ccaaagtccc cgcagccccg
     5101 aggaggtacc atgcgcgtcg cttcctgtcg tccacgactt gtcgcattcg ctgccgtgtc
     5161 gatcctggcg ctcgcggctc ggaccgcctc ggcccagtcc cccctctcct tcgacgtgag
     5221 cctggcgccc aacacctcgg gaaccctgga gggttccgcc tccctgcgcc tcaggtgggc
     5281 ttccttcggg gagtcgggcc tgacgttctc ggcctcgagc ttcctggagg agtatcccga
     5341 gggcgggggc acgaccacgg tcatccagtc ccggaagact ctggacctgg aggtctttcg
     5401 cctgccctcg gacatcctgg ccctgcggtt cggaaggacg ggcggagtct ccctgagccc
     5461 cgtggccctg gtgaacggtg tcatgctgga cgaggacaag tacggggagg atacgggagt
     5521 agtctattac gtaggacagc ggacgatctg ggcgaaaccg ttgctggggg cggacctgaa
     5581 actgtccctg ggtaccgcat cggtggaggt gtactatcga acctcctggc ccttcaccct
     5641 gagggagtac ctggaaggca tcgcgaacta ctcgaccgtc gtcaccccct tctccgtctg
     5701 ggacgagggc ttcgagaccc ggctgggggg tacgttgtcc ttccggcccg cgccgggctg
     5761 gacggtccgg ggcggggtgg actgggtccg acacatcggg tattccgttg cggtggcggg
     5821 aggcgtggct acggatttcg tctacgaaag ccaaagcctg gaaggttccc tgaccctgac
     5881 gttcccggtc ggggagcggc ggcccatggt cggagtggcc tacgcctacg acgccttcct
     5941 gcctgtggac gcgatctacg accagaagtc ctacaccaac ggccgcatcc gcttcctcct
     6001 gggcctggag ctctgagaca tggtagcgcg cgactcccgg gccgcccgga tcgggggtcc
     6061 ccggatcaga tcgggttgat ccgctccgtg atcccgtaga agaggggagg gcagaagcgg
     6121 cggatgaagt agaggagccg ctccggggcc ccgatcagga tctccttgcg ccttcgtcga
     6181 acggcttcaa cgatgatccg ggcgcaccgt tccaccggca tgccccgctc ctgtcccgcg
     6241 tccatcctgc cgtagaccgt tccgtcgcct ttgagcgagt ggatcgagat gtcggtcctg
     6301 atccgtcccg ggcacacgag ggtcacccct acgcccgatc cccggagctc tgcccggagg
     6361 gtctcgaaat agccctgcag ggcgtgcttg gaggcgcagt aggtggagcg catcgggaat
     6421 ccgaacttcc ctgccatgct cccgatcacc acgatccggc cgccgcccgt ctcgatcatc
     6481 cagggaaggg cgttccgggt gacggcgacg gctccgaaga agttgacctc gaagagggcg
     6541 cgctccaccc tgctctccgt ctccaaggcg gtggaccgct ggctgacccc ggcgttgttg
     6601 accaggatgt ccagccgccc gatcgcggac ttcacggccc ctgcagcgcg cagaacggac
     6661 tcgggatccg tcacgtcgca gggtacgaca tgacagcgcc ccgtctcgat cccgtccagg
     6721 atttccttga gctctccgag cttgtccgcg ctccgggccg agaggaccac gacggccccc
     6781 cttcgaaaga actccagcgc cagggccttc ccgatgccgg aggatgcccc ggtgatccag
     6841 accgtcttgc cgccgaattc catgcgcagc ctcccttcac agccggggct tccggtcgtg
     6901 ttcgaatgac gcaaggagca gaatcaccgg ttgtatcgag ccttatggtc aattatgtat
     6961 ccttcgattc atgtactggc aacatggggg cgagcgggct gttggcacgt ttcatccgtt
     7021 tggccaatgc cttggtcctg cggcagaaat tcttaaacgg tttccggagg tagttctcgc
     7081 cggtctgtaa ccattcggcg cataaattac tatattagta ataaaatcgc cggaggcagg
     7141 ccatgtctga gtatcgatcg aatcggctcc gaaccttccg aatacggccc ggacgatcct
     7201 ccccgggccc ccggatctcc gagaccttct ccctggccct gctggccgcc gcactgatcc
     7261 tcgttccgcc gcccgtggcg gcccaggcgg ggggcggagc cgccggggtc tttcggagcc
     7321 gggagcatac gttccggctg gtgaaggtgc tggatcggct ggactacccc tgggccctgg
     7381 ccttcctgcc cgacgggagc atgctgatct ccgagcggac tggaaggctc cttcgggttc
     7441 gggaaggccg ggcccagccc gtctcggggg tcccgacggt gaacgcctcc ggccagggag
     7501 ggcttctgga tctcgccgtg agcccttcct tcgcctcgga ccggaccgtc ttcttcactt
     7561 attcacagcc cggcgccggc ggagcgggca cggccctggc ccgggcggtc ctggacggga
     7621 cgagcctccg ggaggtccgc acgatctgga gcatggagcg gaagaccggg gccggacagc
     7681 acttcgggtc ccggatacgg ctcctgcagg acgggacgat cctcctgacg accggggacc
     7741 ggggacaggg ctcccgcgcc caggacctgg gcgacgacgc gggcaaggtc caccgggtag
     7801 ccccggacgg ctccgtcccc gcggacaatc cgttccgcgg caaccgggcc gcccggccct
     7861 cggtgttttc ctacggccac cggaatcccc agggcctggc gatccatccg gggaccgggg
     7921 aggcctggat caccgagcac ggcccccagg gcggcgacga ggtgaacctc gtccgccccg
     7981 gacgcaacta cggctggccg gtcatcacgt tcggacgcca gtacggctcc ggggcgcaga
     8041 tcggggaggg gacggccaag cccggcatgg agcagcccct gatccactgg accccctcca
     8101 tcgccccctc gggcctggag ttctacaccg gggacgcctt tccccgctgg aaaggcagcc
     8161 tgttctccgg gaacctggcg ggacggcgcc tggttcgcat gaccgtgggc gatgcgcagg
     8221 tcctctccca ggaggtcctg ctggagggct cggtcgggcg gatccgggac gtgcggcagg
     8281 ggcccgacgg attcctgtac atcctgacgg acgagtcccc cggcgccgtc tatcggctgg
     8341 aaccggggga ctgagggagg ggaaccgggg ccctcactgc tggggcatca ccaggtccgg
     8401 cacgaacatc gtgatctccg ggatgaaggt gaccagcatc aggacgatga tcatgacccc
     8461 ccacatgggc aggagggccc gggtgacctg ctccatccgg atcttcccga tggcgcagcc
     8521 cacgaagagg gcggagc
//
LOCUS       DAOTEI010000152         8473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5924_length_8473_cov_4.552744, whole genome shotgun sequence.
ACCESSION   DAOTEI010000152 DAOTEI010000000
VERSION     DAOTEI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_8473_cov_4.552744"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1962)
                     /locus_tag="P5313_12995"
     CDS             complement(37..1962)
                     /locus_tag="P5313_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81330.1"
                     /translation="MKKKTLILAGIAVLLIIAAGIGTFAVLGAGGGADRERQNTLRLA
                     RDYLEQGDFDRALDLLDRLLIKNADDPDARALRDEVVARKKATESDKARAEAEKNRSG
                     QDALTQTISKLGQTIERTSTPPARDTAAEKAAEEEARRKAEAEAAAARKRAEEAELAK
                     KSAELQEKMRAVNELVRKGRESMTNGQYDPARKSFASAQEILPPGEDKFAARTLADIA
                     ESYYDGYKRAPESPDGIQSAKDAQKTAQEAIRKDATDARPHYTLSKIYNDSKLPDLAV
                     KELEEATRLDPKNYLYAFELGKAQYALKKYEEARRSFESAGSLNPQFEPAFFNLGMTQ
                     RVLRNNDAALTAFRTAVKIKPDFVRAHIEIGRVLFAKGDTKGAITSYRTALSFEPKNV
                     TALRDLGLAQVKEGNLADAERTFAEALAVESDAVTNYNLANVKIDLKKFREAVPLAEK
                     AVAAAPNNALYQYTLGLAHDGAGNADGAIAAYTRAAALDKAYAPPRINLGKLYLEAGF
                     DDKALPPLEEAYKIDPNSLEANNNLANAYGRKGLWDKSIFHYEKALQLSPRNPTIRLN
                     LARAYVSAGTLEKARDAYAELIKLAPSEWDAYFELGKVYASLKDSANARRVLTELKAK
                     KPDYEKRAEVDAILAGL"
     gene            complement(1985..2704)
                     /locus_tag="P5313_13000"
     CDS             complement(1985..2704)
                     /locus_tag="P5313_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81331.1"
                     /translation="MSGRRIVRFAVLAALFLSVLSSASAQTGSFAQGERLFLENRPLE
                     ASALLERATRENPRDEKAWLYLGICYQQLGRHDEAVAVFRKGLPAALSLSHVFHFNMG
                     NSFLAQGRNAFAEEMYGQAIQARPDWAPPFLNRANARMAQEKYPDAVADYTVYLSINP
                     STPQAESIRKVIELLGHAQAAEAALKAAEEARKLAEEQARQALLAAVADSLRQSAEDT
                     TSLSAGSGDVQGYTDDFTLED"
     gene            2897..4279
                     /locus_tag="P5313_13005"
     CDS             2897..4279
                     /locus_tag="P5313_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81332.1"
                     /translation="MSFVVILIIVIGAGVGVLAIFLVRMVVTPKQLAAVNDMIKQGRL
                     SAAARIVRGIIAKTPTSAPAHYYLGLVYLAEEKPELALMEFKTVNQIGQFGEEVPEVE
                     FRTRIAGMYEKFSQFEEALKEHILLTKLESSVAEHYFNCGRLFDERGKTDVGVKYLRK
                     ALELDPRMGKAHARLGLILYKTKHPVESRAELEIAIKLQPDYLESYYYLGKLLKESND
                     YTAALLTLEKAQRSPELKVKALVERGGCYMSMGALDKAVVELERAVKLAKDEGAPEAL
                     YARYFLAMCFEKMRNLDRAIEQWEKIYARKPGFRDVAEKLTQYQEYRSDDKMKDYLTC
                     GREEFIELCKAITQQALQLNIRDVSMTPNGVDMIAVENDSEKWMGTKKMPRLLRFLRV
                     SEMLDDSSIRSLLDQMKKLSIIKGSIITSSSFNRAALEFGENRAVELTAKDQLQELLA
                     KTTLPSGSRR"
     gene            4276..4956
                     /locus_tag="P5313_13010"
     CDS             4276..4956
                     /locus_tag="P5313_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY81333.1"
                     /translation="MRILCVSDQVDPVVYSSAIRDRFKDVDLVLAAGDLPMDYLSFIV
                     SGLNKPLLFVFGNHNLEELAYYRPRADVRGHPVDTPWPSRDRLEGSGAVYTGFRVVRE
                     GGLLVAGLGGSRWYNGGANQFTNLQMAFRILFLIPSLVWNRIFHGRYLDILLTHAPPE
                     GIQDRPDPCHRGFRPFLWFMRAFRPRFLVHGHVHLYDMASSRVSEYGGTTVVNAFSHY
                     LLDWEDRT"
     gene            4956..5858
                     /locus_tag="P5313_13015"
     CDS             4956..5858
                     /locus_tag="P5313_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRY81334.1"
                     /translation="MDLHSERAEDDFSRARTREFLTRVQGFLDPSRSRLLSLEEVRSV
                     LRPEGETYLGMQAVPLDRIVGSEGRYRDFNRHFLPRFDHLRPRWVRVDVAHYKDVILP
                     PIQLYEAGGLYFVRDGNHRVSVARMQGIQAIDAEVVSLDTKVSLEPGMGLEDLRKAVL
                     RYEKDRFYGKTGFLDATGDGNLDFSAPGQYDIVLDHILVHKYFINEGKPEEIPFRDAL
                     ESWYRTVYAPIAAVIRAERIDARFPGRTVADLYVYIVKHWDHLKRKYGLPVTARSAAV
                     DFSSRYGQGFRDRVRGVLSALLGR"
     gene            6054..6224
                     /locus_tag="P5313_13020"
     CDS             6054..6224
                     /locus_tag="P5313_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81335.1"
                     /translation="MAAISKNARTSSTTQRFYCPCGGEIKMKTLFEDGKIKNIAECEK
                     CKRTERRPSDFS"
     gene            complement(6299..7081)
                     /locus_tag="P5313_13025"
     CDS             complement(6299..7081)
                     /locus_tag="P5313_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY81336.1"
                     /translation="MLSSIWFMVCAFSLFLLYDVLLKVARALAPDRGLELSFRLADEI
                     IRRLFVTLRWIGGFRYLPEGTPGGDLPDRFLIVANHQSLLDIIVVMSYFGSCRGVRLV
                     SKRELGRFVPLVSSVLRIQGHALISRKGEAKQAMDELARFARLCAREGVNPVIFPEGT
                     RSRTGEVGPFHSAGVRRILEEGGSLPVVALAIDGGWRVRNVRGLLQNLAGGRYRVRPA
                     GIYPAPAGKREILDVLARARASISEIVDRWHAEQPSPRQDMG"
     gene            7246..7515
                     /gene="rpsT"
                     /locus_tag="P5313_13030"
     CDS             7246..7515
                     /gene="rpsT"
                     /locus_tag="P5313_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRY81337.1"
                     /translation="MGKNQSSAEKRHLQSEKRRVRNKRAKSEIRTEAKKVVAAVQGKD
                     KAGAEAALKAMIKDIDTAASKGIVKRNTAARKKSRMQRLVNTLTA"
     gene            7580..7924
                     /locus_tag="P5313_13035"
     CDS             7580..7924
                     /locus_tag="P5313_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRY81338.1"
                     /translation="MMAEKVTKAELIDSLHERLSISRKDIHSLIDGVFEEIKTAILSG
                     RTVELRGFGTFEVRVRKGRKRARNPKTGEPVSVADHGVAAFRPGRDLKKAAWNLKDRP
                     GPVPNGSDGSGE"
     gene            7899..>8473
                     /locus_tag="P5313_13040"
     CDS             7899..>8473
                     /locus_tag="P5313_13040"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81339.1"
                     /translation="MAPTAPGNNKPIGPVPTAACFLVSLALYILAFPNPVLEKGLGFL
                     AWIALAPLFAALADRKARSAAVLGAAFGISFYALSNYWLWIYHPAAMIIACAYAALCL
                     AILFPVISLAFRAFPKAGFAALPFLWLSYEFLRSRGFMGYPYGVLGYAFWRDPAMLGL
                     ASAGGVWLVSLAALGANAAAGFALRELLRGRP"
BASE COUNT         1398 a         2761 c         2744 g         1570 t
ORIGIN      
        1 aggttgttga aatgcttcga gtatttcaac aacctgctag agacccgcca ggatggcgtc
       61 cacctccgcc cgcttctcgt aatccggctt cttggccttg agctccgtca gcacccgccg
      121 tgcgttggcg gagtccttga ggctggcgta gaccttcccg agctcgaagt aagcatccca
      181 ctcggagggg gccagcttga tcagctccgc gtaggcgtcc cgggccttct ccagggtccc
      241 cgcgcttacg taggcccggg ccaggttcag ccggatggta ggattccggg gggaaagctg
      301 gagggccttc tcgtagtgga agatactctt gtcccaaagc cccttgcggc cgtaggcgtt
      361 ggccaggttg ttgttggcct ccagggagtt cgggtcgatc ttgtacgctt cctccagggg
      421 aggcagggcc ttgtcgtcga aacccgcttc caggtagagc ttgcccaggt tgatgcgggg
      481 cggggcgtag gccttgtcca gggcggcggc tcgggtatag gccgcgatcg ccccgtccgc
      541 gttcccggcc ccgtcgtggg ccagacccag ggtgtactgg tacagggcgt tgttcggggc
      601 cgcagcgacg gccttctccg ccaggggcac ggcttcccgg aatttcttga ggtcgatctt
      661 gacgttggcc aggttgtagt tcgtgaccgc gtcggactcg acggccaggg cctccgcgaa
      721 ggtcctctcc gcgtccgcca ggttcccttc cttgacttgg gccagaccga ggtcccgtag
      781 ggccgtcacg ttcttcggct cgaaggagag ggccgtccgg taggaggtga tggccccctt
      841 ggtgtctccc ttcgcgaaca ggacccgccc gatctcgata tgggcacgga cgaaatcggg
      901 cttgatcttg accgcggtcc ggaacgccgt cagggcggcg tcgttgttcc ggaggacccg
      961 ctgggtcatg cccaggttga agaaggccgg ctcgaactgg ggattcaggg aacccgcgga
     1021 ctcgaaggac cttcgggcct cctcgtattt cttcagggca tattgggcct tcccgagctc
     1081 gaaggcgtac aggtagttct tgggatccag ccttgtggct tcctccagtt ccttcacggc
     1141 caggtccggg agcttggaat cgttgtagat cttgctcagg gtgtaatgcg ggcgggcgtc
     1201 cgtggcgtcc tttcggatgg cctcctgggc cgtcttctgg gcgtccttgg cggactggat
     1261 gccgtccgga ctctcggggg cccgcttgta cccgtcgtaa taggattccg cgatgtccgc
     1321 cagggtccgg gccgcgaact tgtcctcccc ggggggaagg atctcctggg cggaggcgaa
     1381 ggacttgcgg gcggggtcgt actgcccgtt cgtcatggac tcccgacctt tgcggaccag
     1441 ctcgttcacg gcccgcattt tttcctggag ctccgcgctc ttcttggcca gctccgcctc
     1501 ctcggcgcgt ttccgggcgg ccgcggcctc cgcctcggcc ttgcgccggg cctcctcctc
     1561 cgcggccttc tccgccgccg tgtcccgggc gggcggggtc gaggtacgct cgatggtctg
     1621 gccgagcttg gagatggtct gggtcagggc gtcctggccc gagcggttct tctccgcctc
     1681 ggcccgggcc ttgtccgact cggtggcctt cttccgggcc acgacctcgt cccggagggc
     1741 acgggcgtcc gggtcgtccg cgttcttgat gagaaggcgg tccaggaggt ccagggcgcg
     1801 gtcgaagtcc ccctgctcca ggtagtcccg ggccaagcgc agagtgttct ggcgctcccg
     1861 gtccgccccg cccccggccc cgagtacggc gaaggtcccg atacccgcgg cgatgatcag
     1921 aaggacggcg atgccggcca gaatgagcgt tttcttcttc acgatgattc tccgtttcgt
     1981 tccgtcagtc ttccagggtg aagtcgtcgg tatagccctg cacgtccccg gaaccggcgg
     2041 acaggctggt ggtatcctcc gcgctctgcc ggagggaatc ggccaccgcg gccaggaggg
     2101 cctgccgggc ctgctcctcc gccagctttc tggcctcctc cgcggccttg agggccgcct
     2161 cggcggcctg ggcgtggccg agcagttcta tcaccttgcg gatggattcc gcctgggggg
     2221 tcgaggggtt gatgctgagg tacacggtgt agtcggccac cgcgtccggg tacttctcct
     2281 gggccatccg ggcgttggcc cggttcagga agggaggggc ccagtcgggc cgggcctgga
     2341 tggcctggcc gtacatctcc tccgcgaagg cgttccggcc ctgggccagg aagctgttcc
     2401 ccatgttgaa gtggaacacg tgggacaggg agagggccgc gggcaggccc ttgcggaaga
     2461 ccgccacggc ctcgtcgtgg cgcccgagct gctggtaaca gatcccgagg tagagccagg
     2521 ccttctcgtc ccgggggttc tctcgggtcg cgcgttccag gagggcggag gcctccaggg
     2581 gtctgttctc caggaagagc cgttctccct gggcgaagga tccggtctgg gcggaggcgg
     2641 aggagaggac ggaaagaaag agagcggcca ggaccgcgaa gcggacgatc ctgcgtcccg
     2701 acaaggccgc ggatcctttc ctccgtccgt gtagcaccct tatgcacccc cgttcctgct
     2761 gacaagatcg gcgtatctgc tatactattt gagtataccg cgcctcgggc tatacgaaaa
     2821 gtaggacggc ctagccggac cggacgcgtc gaactcggcg cggacttcgg ttccgggacg
     2881 tccgacagga gacgggatgt cctttgtcgt gatcctcatc atcgtgatcg gtgcgggagt
     2941 cggcgtcctg gccatcttcc tggtccgcat ggtcgtgacc cccaagcagc tggccgccgt
     3001 gaacgacatg atcaagcagg gccgtctttc cgccgcggcc cggatcgtgc gcggcatcat
     3061 agccaagacc cccaccagcg ccccggccca ctactacctg ggcctcgtgt acctcgcgga
     3121 ggaaaagccc gagctggccc tgatggagtt caagaccgtc aaccagatcg gccagttcgg
     3181 ggaggaggtt ccggaggtgg aattccggac ccgcatcgcg gggatgtacg agaaattcag
     3241 ccagttcgag gaagccctca aggagcacat tctcctgacc aagctggaga gttccgtcgc
     3301 ggaacactac ttcaactgcg gccggctctt cgacgaacgg ggcaagacgg acgtgggggt
     3361 gaagtacctt cggaaagccc tggagctgga tccccggatg ggcaaggccc acgcgcgcct
     3421 cgggcttatc ctctacaaga ccaagcaccc cgtcgagtcc cgggcggagc tggagatcgc
     3481 catcaagctc cagcccgatt acctggagtc ctattactac ctcgggaagc tcctgaagga
     3541 gagcaacgac tataccgccg ccctcctgac cctggagaag gcccagcgga gcccggagct
     3601 caaggtgaag gccctggtcg agcggggcgg ctgctacatg agcatgggcg cgttggacaa
     3661 ggccgtggtg gagctggagc gggcggtcaa gctggccaag gacgagggag ccccggaagc
     3721 cctgtacgcc cgctacttcc tggccatgtg cttcgagaag atgcggaacc tggaccgggc
     3781 catcgagcag tgggagaaga tctacgcccg caagcccggc ttccgggacg tcgcggagaa
     3841 gctcacccag taccaggaat accggtccga cgacaagatg aaagattacc tgacctgcgg
     3901 gcgggaggag ttcatcgaac tctgcaaggc catcacccag caggccctgc agctcaacat
     3961 acgggacgtg tccatgaccc cgaacggggt ggacatgatc gccgtggaga acgattccga
     4021 gaagtggatg ggcaccaaga agatgccgcg cctgctccgc ttcctgcggg tgtccgaaat
     4081 gctggacgac tcctccatcc gttccctcct ggaccagatg aagaagctca gcatcatcaa
     4141 gggttccatc atcaccagct cgtccttcaa ccgcgctgcc ctggagttcg gggaaaaccg
     4201 ggccgtcgaa ctgacggcca aggaccagct ccaggaattg ctggcgaaaa cgaccctgcc
     4261 ttccggctcc cgtagatgag gatcctctgc gtctcggacc aggtcgatcc ggtggtgtac
     4321 tcctcggcca tccgggaccg gttcaaggac gtggatctcg tcctggccgc cggggacctt
     4381 cccatggact acctgtcctt catcgtctcg ggattgaaca agcccctgct cttcgtcttc
     4441 gggaaccaca atctggagga gctggcgtac taccggcccc gggctgatgt ccgcggccac
     4501 cccgtggaca ccccctggcc gtcccgggac cgtctggagg gttccggggc ggtctacacg
     4561 ggtttccggg tggtacggga gggcgggctc ctggtggccg ggctgggggg ctcccgctgg
     4621 tacaacggcg gcgccaacca gttcacgaac ctccagatgg ccttccgcat cctttttttg
     4681 atcccgtccc tggtatggaa ccggatcttc catggccggt acctcgacat cctgcttacc
     4741 cacgcccctc cggaaggcat ccaggatcgg ccggacccct gccatagggg tttccgcccc
     4801 ttcctgtggt tcatgcgggc cttccgcccc cgcttcctgg tccacggcca tgtccacctc
     4861 tacgacatgg catcctccag ggtttcggaa tacggcggga ccacggtggt gaacgccttc
     4921 agccattatc tgctggactg ggaggaccgg acctgatgga tcttcattcc gagcgagccg
     4981 aggacgactt ttcccgggcc cggacccggg aattcctgac ccgggtccag ggtttcctgg
     5041 atccctcccg aagcagactc ctgtccttgg aggaggtgcg ttccgtcctg aggcccgagg
     5101 gcgagaccta cctgggcatg caggccgtgc ccctggaccg gatcgtcggc agcgaaggtc
     5161 ggtataggga tttcaaccgc catttcctgc cccggttcga ccacctccgg ccgcgctggg
     5221 tgcgggtgga cgtggcccat tataaggacg tcatcctgcc gcccatccag ttgtacgagg
     5281 cgggagggct ctacttcgtc cgggacggca accacagggt ttccgtggcc cggatgcaag
     5341 gcatccaggc catcgacgcg gaggttgtgt ccctggatac caaggtttcc ctggagcccg
     5401 gcatgggcct ggaggacctc cggaaggccg tcctgcggta cgaaaaggat cggttctacg
     5461 ggaagacggg cttcctggac gccacagggg acggaaacct ggatttctcg gccccagggc
     5521 agtacgacat cgtcctggac catatcctag tccacaaata cttcatcaac gaggggaaac
     5581 cggaggagat ccccttccgg gacgccctgg aatcctggta ccggaccgtc tacgccccca
     5641 tagcggcggt catccgggcc gaacgtatcg acgcccggtt cccgggtcgg accgtggcgg
     5701 acctgtacgt ctacatcgtg aagcactggg accacctgaa gcggaagtac ggactgccgg
     5761 tgacggcccg ttccgcggcg gtggacttct cctcccgcta cggccagggc ttccgggacc
     5821 gggtccgggg ggtattgtcc gcgttgctgg gaagatgacc tttcttgtaa cctcccccgg
     5881 ggcacgttgt ttctcgtatg catggggtga gcgagattgc cttctcgact ccgttatgtg
     5941 gcgcggctcc cgggggttcc tcctttcccc cgggagcatt tttttgcgcc tctcccgcgg
     6001 ttgacatttc cgagccttta aggatagatt ccatccttga ttaaggagtt gccatggcag
     6061 ccataagcaa gaacgcccgt acttccagca cgacccagcg cttctactgt ccctgcggcg
     6121 gggagataaa gatgaagacc ctcttcgagg acggcaagat caagaacatc gccgaatgcg
     6181 agaagtgcaa gcgcacggag cgccggccca gcgatttcag ctagtccagg gtccgcggaa
     6241 ccgtacggtc cgatccgggg cgccgggagg cttgaagcct tcggcgtccc gctttttttc
     6301 agcccatatc ctgacgcgga gacggctgct ccgcgtgcca acggtcgacg atctcggaga
     6361 tcgaggcccg ggcccgggcc aggacgtcca ggatctcccg tttgccggcc ggcgccggat
     6421 agatccccgc cggacggacc ctgtaccggc cgcccgcgag gttctgcaga agtccccgta
     6481 cgttccggac cctccacccc ccgtcgatgg ccagggccac gacgggaagg cttccgcctt
     6541 cctccaggat ccggcgcacc cccgcggaat ggaagggacc cacctcgccg gtccgggacc
     6601 gggtgccctc ggggaagatg accgggttca cgccctcccg cgcgcagagc cgggcgaacc
     6661 gcgccagctc gtccatggcc tgtttggcct ctcccttccg ggagatcagg gcgtggccct
     6721 ggatccgcag gaccgaggac accaggggga cgaagcgccc gagttcccgc ttggatacca
     6781 ggcgaacccc ccggcaggat ccgaaatagc tcatcaccac gatgatatcc aggaggctct
     6841 ggtggttcgc gacgatcagg aaccggtcgg gcaggtcccc gcccggggtt ccctcgggaa
     6901 ggtagcggaa ccccccgatc catcgcaggg tcacgaacag gcggcggatg atttcgtccg
     6961 ccaggcggaa cgacaattcc aaccctcggt ccggagccag ggcgcgggct accttcagga
     7021 gcacgtcgta gaggaggaag agggagaagg cgcagaccat gaaccagatc gaggagagca
     7081 tggctccatg atagccgctt ctcccggaaa ccgcaactcg aggatcggat tccaaatgcg
     7141 tctatctatt gacacggcaa ggcgcatctg ctagtttttc cctcaagccc cgcatcggcg
     7201 ccgaggcgcc gcgggggagt ccggaaccgt tttaggagta cttagttggg caagaatcag
     7261 tcgtccgcgg aaaaacgcca cctccagtcc gagaagcgcc gggtccgcaa caagcgcgcc
     7321 aagagcgaaa tccgcacgga agccaagaag gtggtcgccg ccgttcaggg caaggacaag
     7381 gccggggccg aggccgccct caaggccatg atcaaggata tcgacaccgc ggccagcaag
     7441 ggcatcgtca agagaaacac cgcggcccgc aagaagtcgc ggatgcagcg cttggtcaac
     7501 accctgacgg cttgatccag gaaacgccgc gattcgacgg acggcgtcgt tcttttccga
     7561 gcgctaggtt ccggggggca tgatggccga aaaggtcacg aaagctgagc tgatcgattc
     7621 tctccacgag cgcctttcga tcagccggaa ggacattcac tccttgatcg acggcgtatt
     7681 cgaggaaatc aagacggcca tcctgtccgg ccgcaccgtc gagctccgag ggttcggcac
     7741 tttcgaggtc cgggttcgca agggtcggaa acgcgcccgg aacccgaaga ccggggaacc
     7801 ggtctccgta gccgaccacg gcgtggcggc cttccgcccc ggccgcgatc tgaaaaaggc
     7861 cgcctggaat ttgaaggacc ggccgggacc ggttccgaat ggctccgacg gctccgggga
     7921 ataacaagcc gatcgggccc gtaccaaccg ccgcctgctt cctggtttcc ctcgccttat
     7981 atatcctcgc cttcccgaac cccgtccttg aaaagggcct gggtttcctc gcctggatag
     8041 ccctggctcc gctcttcgcg gccctggccg accggaaagc ccggtccgcg gcggtcctgg
     8101 gggccgcctt cgggatttcc ttctacgccc tttccaacta ctggctctgg atctatcacc
     8161 ccgcggccat gatcatcgcc tgcgcctacg cggccctctg cctggcgatc ctcttcccgg
     8221 tcatctccct cgccttccgg gccttcccca aggccgggtt cgccgccctc cccttcctct
     8281 ggctgtccta cgaattcctc cgctcccggg ggttcatggg atacccctac ggcgtcctgg
     8341 gctacgcatt ctggagggat ccggccatgc tgggcctggc gtccgcgggc ggggtctggc
     8401 tcgtgtccct tgccgccctg ggggccaacg ccgccgctgg attcgccctg cgggagctcc
     8461 tgcgtggacg tcc
//
LOCUS       DAOTEI010000153         8390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_5994_length_8390_cov_5.259268, whole genome shotgun sequence.
ACCESSION   DAOTEI010000153 DAOTEI010000000
VERSION     DAOTEI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_8390_cov_5.259268"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="P5313_13045"
     CDS             complement(<1..642)
                     /locus_tag="P5313_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY81340.1"
                     /translation="MTTVLLVDDKENIRTVMGAVIRREGFEVLTAADGREALDLALER
                     TPDLVLSDVRMDGMSGTELFRELRSRGISAPFVFMTAYASVPDAVDAIRDGAVHYLTK
                     PVDYGELLRIIRRAVLHKSGEAQAERSSSRSILGSGPAVRKLLARIEAVAASEATVLI
                     RGENGTGKELAARAIHSRSPRRNGPFVPVHCAALNPNLLESELFGHERGAFTGA"
     gene            complement(639..1997)
                     /locus_tag="P5313_13050"
     CDS             complement(639..1997)
                     /locus_tag="P5313_13050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY81341.1"
                     /translation="MPLGHKLVLFALALLALLSAQWGASIARERSLLMEEAQERAAVL
                     VRNMAELAREPIASGRLGSLDRQIRSFLGERDVTYARILDHRSRVLAASEDRLVGWSI
                     SGPPEPEGFLRWTDVGLVARAPIRIPGQGDGYVELGMDRGPLDAKIRRSLGILARFLA
                     AELALFLAFMILLYRQLFEPVRALVARLGQARPEAGYEPIDLPRRAAPEIARIARAVD
                     ELRMRAAEYQAELVAEERLATIGRMAADLAHEIRNPLEAISGAVEALAGAVPQDNEFT
                     RVIREEVRNLNEYLAGILEFARKGGRRPEAADLADLIREAAALAAPRAREARVLFDLR
                     LSYCPCVVSRSAVKRAVLNLLLNAVEASAPGQAVEAATERFENSVVLRVRDRGSGVAP
                     SDRMRLFEPYFTTKPGGTGLGLALARQTVEEHGGTLTLESADGDGGTQAILRLPAREE
                     RE"
     gene            complement(1981..2772)
                     /locus_tag="P5313_13055"
     CDS             complement(1981..2772)
                     /locus_tag="P5313_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HRY81342.1"
                     /translation="MRALACILLSLACLTAAADPPLRLALEAGADGLRTAQDWQAAAR
                     EYLSRTGVPLEPLLVSSSEEMLRALDSREAEAAVLDAAAFLRNRDRLRILGVAAEYGS
                     PYERFLLVASAESVIHRPEDLPRARLVLAGTPGTLPWDLPLAWARRYRGPDAGAADPR
                     PETGYEGVLRNVALGQADAGFLPGGFLERRKTSALADRIRIVAAIPPCPLALLVARRD
                     LAGDRAALAEGLLGLSAGTLELRTPPADLEAVLSGLEEALRAPRP"
     gene            2987..4372
                     /locus_tag="P5313_13060"
     CDS             2987..4372
                     /locus_tag="P5313_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY81343.1"
                     /translation="MTITLSGTRKWMFSLGDLTAAVPLTILGFFQLYFLTDVARIPPG
                     MAAWSILVVKLWDAVNDPVLGALSDRFRSRGGRRRVPMLAASVPLGLTFCLLWIVPPF
                     RPLGAAVWYAIVFLVFDTCFTMFHVSFNALTPVLARDYDERSSLNGYRMVFSIAGTLA
                     AVILMTVLSGRIEDARARFALAGIGLGLFCMIPPWFAYKAAEGFDEEGACETPGSGRM
                     RPGFLEALAESTRAVLANRPFRQVMGLYLFSWTTTAVISSALVYFVSYYLRAPAHSNY
                     LVLAAEVSAIAFIPLVVAAARRWDKRRAFMAGCAAWAAVQGAISLLAPGQLVPAYALA
                     VLIGLGIATAYVLPWAMIPDVIELDTAASGRNREGSYYAFASFFQKLGTGAALWLMAR
                     ALEAGGYVTPTAAVPFPEQPQSAIRAIRLFMGLAPAVLLACATAFAWLYPVSREDQRA
                     ARDAILARAGE"
     gene            4369..5217
                     /locus_tag="P5313_13065"
     CDS             4369..5217
                     /locus_tag="P5313_13065"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY81344.1"
                     /translation="MTPPASPPGRRRFGPPWPESPGRRSTVSPGPAACLRGLLRLASG
                     AVFLAGLWVFAASVEAAADPGTRDKRMRLLRMRGARYLSSLLGVIRVRRGRPSEGTFL
                     AAGNHISWLDSLVVVAETGAAYTAYSRDSRLVDSFLRVMGTILLPRGYAASLPGAVKA
                     AAERLRAGCPVGVFPEGTTTFGPGCLPFKPAFFQAALDTGVPVQPFALTYETPVPWPS
                     APRSVHWVDWTPLPLHVFRVLCLPRIEARVAWLPAILPEAGEDRRGLASRTEAAVRVG
                     LAAGAA"
     gene            complement(5234..5689)
                     /locus_tag="P5313_13070"
     CDS             complement(5234..5689)
                     /locus_tag="P5313_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRY81345.1"
                     /translation="MGYATFGEVLEFAIGRENAAVDFYRELSGIAAFAAQKSTLAEFI
                     AMERGHAAMLEAMKARGTVKLSASTAVDLGAARRVEATDRPPAGMTFQDILTAAASKE
                     ARSFELYGRLAREAGDPGIRETFERLAGEESRHRAYFQDLYEREVARDN"
     gene            complement(5691..7466)
                     /locus_tag="P5313_13075"
     CDS             complement(5691..7466)
                     /locus_tag="P5313_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY81346.1"
                     /translation="MDIKDIIAPFAVWKRAFEKPFTVRKPIDERPGAPRYRGFHINDA
                     DTCVGCGTCESICMNKAIDLVPVEGRSPSGGDSGLRPRVDYGRCCWCALCVDICPSGS
                     LGMSNEYLWIDSDPEVFRYVPGVDPKPWDGAELGYRRAEGYRLLDMERVPMPELSHAE
                     GVKSFLELVKGYSREQAEREADRCVACGVCVASCPAHMDIPGYINAVRGGDLETGLRL
                     LYETNPFPEACGRICTRACESACAIGSGGDPVAIRWLKRYIADQVELADYGKSLPRAE
                     SATGRKVAVVGAGPGGLSAAYYLALLGHKVTVFEKDEAPGGMLRYGIPEYRLPYPALD
                     KDVDYIRSLGVEIRHGATVGKDPALAKLREDFDAVYLSTGLPSAYRLDVPGDEHPRVL
                     DGVAVLAAATRGEELGLGRRVAVVGGGNVAMDAARTALRRGCEVDIVYRRRQVDMPAD
                     PEEIREAKAEGARLIERAIPLRVEDSGDGGARFVWNEAEMVQKEPGKRPVPEPIPGAV
                     VTERYDSIIAAIGQGPELSYITEADGVEVRRMKPVTDRTGRTSVPTVFAGGDLVNEKK
                     DAISAIADGHRAARAIDRILGGRGD"
     gene            complement(7471..>8390)
                     /locus_tag="P5313_13080"
     CDS             complement(7471..>8390)
                     /locus_tag="P5313_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRY81347.1"
                     /translation="RIAVPEPDFNEYCYAAALEELAGIEVPDKAVWLRTLSLEMIRLA
                     SFLMWIGGQAGAFGHGTIAQWSVTMRDYVLDLFEELSGGRIYHMYIIPGGVRADVPPG
                     FLRHVLETMTVVEQNLKDIELVMFHNAVMKMRAVGLGYIPPDWVDRYGITGPNARAAG
                     FKRDLRKDRGYLAYPRLSFEPVVGAESDAFERARLRLAEMFQGIDLIRQIVDFLPSEG
                     PFRERLPNPLHWKIPAGETYVAAECTRGEYGFYVVSDGGDKPRRVAVRGPSYTHAVSV
                     MERLVRGVNIADVAGLMVSLHTYPPEIER"
BASE COUNT         1172 a         3020 c         2867 g         1331 t
ORIGIN      
        1 ggcgcccgtg aaggcgcccc tctcgtggcc gaagagctcg gactccagga ggttgggatt
       61 cagggccgcg cagtggaccg gaacgaaggg gccgttccgg cgggggctcc gggaatggat
      121 ggcccgggcg gccaattcct tgcccgtacc gttctcgccc cgtatcagga ccgtcgcctc
      181 cgaagccgcg accgcctcga tccgggctag gagctttcgc acggcgggac cggatcctag
      241 aatgctccgg gaggaggacc gctcggcctg ggcctccccg gacttgtgca ggacggcccg
      301 gcggatgata cggaggagtt ccccgtagtc cacgggcttg gtcaggtagt ggacagcccc
      361 gtcccggatc gcgtctaccg cgtcgggcac ggaggcgtag gctgtcatga acacgaaggg
      421 cgcggagatg ccccgggacc ggagctcccg gaagagctcc gtgccgctca ttccgtccat
      481 tcgtacgtcc gagagcacca ggtcgggggt cctttcaagg gccagatcca gggcttcccg
      541 gccgtccgcc gccgtgagca cctcgaaccc ctccctccgg atgaccgccc ccataacggt
      601 acggatgttc tccttgtcgt ccaccagaag taccgtagtc actctcgctc ctccctcgcg
      661 ggaagccgca ggatcgcctg tgtcccgccg tccccgtccg ccgattccag ggtcagggta
      721 ccgccgtgct cctccaccgt ctggcgggcc agggccaggc ccaggcccgt gccgccgggt
      781 ttggtcgtga aatagggttc gaacaggcgc atccggtccg agggagcgac gccgcttccc
      841 cggtcccgaa cccggaggac gacggagttc tcgaatcgct ccgtagccgc ctccaccgcc
      901 tgtcccggcg cggaggcttc tacggcgttc agcaggaggt tcaggacggc ccgcttcacc
      961 gccgaccggg agacgacaca ggggcagtag ctcagacgca ggtcgaacag gaccctcgcc
     1021 tctcgggccc ggggggccgc cagggcggcc gcctcccgga tcaggtccgc caagtccgcg
     1081 gcctccggcc gtcttccgcc cttccgtgcg aactccagga tccccgcgag gtattcgttg
     1141 aggttccgga cctcctcacg gatcacccgg gtgaactcgt tgtcctgggg gacggccccc
     1201 gccagggctt ccacggctcc ggagatcgcc tccagggggt tccggatctc gtgggccagg
     1261 tccgcggcca tccggccgat cgtggccaga cgctcttcgg cgacaagctc cgcctggtac
     1321 tccgcggccc gcatccggag ctcgtccacc gcccgggcga tgcgggcgat ctccggggcg
     1381 gcccgccggg gcaggtcgat cggctcgtac cccgcctccg gtctcgcttg tcccagccgg
     1441 gctaccaagg cccgcacggg ctcgaagagc tgtcggtaca ggaggatcat gaaggccagg
     1501 aaaagggcga gttccgcggc caggaaacgg gccaggatgc ccaggctcct ccggatcttt
     1561 gcatccaggg gcccccggtc catccccagc tccacgtatc cgtccccctg accgggaatt
     1621 cggatgggcg cacgggctac cagcccgacg tcggtccacc gcaggaaccc ctccggttcg
     1681 ggagggcccg agatcgacca tccgaccagc cggtcctcgg aggccgcgag gacccgggac
     1741 cggtggtcca ggatccgggc gtaggtgacg tcccgctccc ccaggaagga ccgaatctgg
     1801 cgatccaggg accccaggcg gcccgaggcg atgggttccc gagccagctc ggccatgttc
     1861 cggaccagga ccgccgcccg ttcctgggcc tcctccatga gaaggctccg ttcccgcgcg
     1921 atggaggccc cccactgggc cgacaggagc gccagaaggg ccagggcgaa caggacgagc
     1981 ttatggccga ggggcacgga gagcctcctc caatccggaa aggacggcct ccaggtccgc
     2041 gggcggggtc cggagctcca gggtacccgc cgagagcccc aggagaccct ccgcgagggc
     2101 cgcccgatcc ccggctaggt cacgacgggc taccagaagc gcaagcgggc agggcgggat
     2161 cgccgccacg atccggatcc ggtcggccaa cgcgctggtc ttccttctct cgaggaagcc
     2221 tccgggcagg aagcccgcgt ccgcctggcc cagtgcgacg tttcgcagga ctccctcgta
     2281 gcccgtctcc ggccgcggat ccgcggctcc ggcatccgga ccacggtagc gccgggccca
     2341 ggccaggggc aggtcccagg ggagggttcc gggagttccg gcgaggacca gccgggcccg
     2401 gggcaggtcc tcgggccggt ggatgaccga ctcggcgctg gccaccagga ggaacctctc
     2461 atagggggac ccgtactccg cggcgacccc gagaatccgc agccggtccc ggtttctcag
     2521 gaaggcggcg gcgtccagga ccgccgcttc cgcctcccgg gagtccaggg ctcggagcat
     2581 ctcctcggac gacgacacca ggagaggctc cagggggacg ccggtacggg acaggtactc
     2641 ccgggcggcg gcctgccagt cctgggcagt ccggaggccg tccgccccgg cctccagcgc
     2701 cagccgaagg ggcggatccg cggccgcggt tagacaggcc agagacagga ggatgcaggc
     2761 cagggcgcgc atagggagat tctggaccgg atcggcggct ctgtcaaacc gcccgtacgg
     2821 gacggcgggg ttgctcccgg ccccggtatc cggaccggcg gctgcttaag gcatgacccg
     2881 gatagggatg gaatgaaggg tggacgagag ctgatacgta acatgacatc cgatgtcgac
     2941 atgttcttct tgcttgcccc ggaaaccgcc cctataatcc tcttccatga cgatcacctt
     3001 gtcgggcacc cgaaaatgga tgttcagtct gggagacctt accgccgcgg tccccctgac
     3061 gatcctggga ttcttccagc tctacttctt gaccgacgtg gcccgaatac ctccgggcat
     3121 ggcggcctgg tccatcctgg tagtcaagct ctgggacgcg gtcaacgatc cggtcctggg
     3181 cgccctgtcg gatcgcttcc gatcccgggg cggtcgccgg agggtgccca tgcttgcggc
     3241 gtccgttccc ctgggtctca ccttctgcct tctttggatc gtgcctccct tccggcccct
     3301 cggcgccgcc gtctggtatg ccatcgtctt cctggtcttc gatacctgct tcacaatgtt
     3361 ccatgtcagc ttcaatgccc tgacccccgt cctggcccgg gactacgacg aacggagctc
     3421 cctgaacggc taccggatgg tgttctccat cgcgggcacc ctggccgccg tcatcctgat
     3481 gaccgtgctc tcgggccgga tcgaggacgc gcgtgcccgg ttcgccctgg cggggatcgg
     3541 cctgggtctc ttctgcatga tccccccatg gttcgcctac aaggccgcgg aaggcttcga
     3601 cgaggagggc gcctgtgaaa cccccggttc gggaaggatg cgcccgggtt tcctggaagc
     3661 tctggcggaa tcgacccggg ccgtgctggc caaccggccg ttccggcagg tgatggggct
     3721 ctacctgttc tcctggacca ccaccgcggt catctcctcc gccctggtat acttcgtcag
     3781 ctactacctc cgggcccccg cccactccaa ctacctggtc ctggcagcgg aagtttcggc
     3841 catcgcgttc atacccctgg tcgtagccgc cgcacggcgc tgggacaagc ggcgggcctt
     3901 catggcgggc tgcgcggcct gggcggcggt acagggggcc atcagcctcc tggcccccgg
     3961 ccagctggtc ccggcatacg cgctggccgt cctgatcggc ctggggatcg ccacggccta
     4021 cgtccttccg tgggccatga tcccggatgt catcgaactg gataccgcgg cctcgggccg
     4081 gaaccgggaa ggctcctact acgccttcgc ctccttcttc cagaaactgg gtaccggtgc
     4141 cgccctctgg ctcatggccc gtgccctgga agccgggggg tacgtcacgc cgaccgccgc
     4201 cgtacccttc ccggaacagc cccagtccgc aatccgggcg atacggctct tcatggggct
     4261 ggcccccgcg gtcctgctcg cctgcgcgac ggccttcgcc tggctgtacc cggtcagccg
     4321 ggaggaccag cgggccgccc gggacgccat cctggcccgg gccggggaat gacgcccccc
     4381 gcgtctcctc ccggccgccg ccgcttcggc ccgccctggc ccgaatcgcc ggggcgcagg
     4441 tccacggttt cccccgggcc tgcggcctgc ctccggggcc tgctccgcct tgcctccgga
     4501 gccgtcttcc tggccggact ttgggtcttc gcggcttcgg tggaggcggc cgcggacccc
     4561 gggacgcggg acaagaggat gcggctcctc aggatgcggg gcgcccggta tctctcgtcc
     4621 ctgctggggg tgattcgggt ccgtcggggg agaccctccg agggcacgtt cctggccgcg
     4681 ggcaaccata tttcctggct ggacagcctg gtcgtggtag ccgaaaccgg ggcggcctac
     4741 acggcctatt cccgggacag ccgcctggtg gattccttcc tccgggtcat gggaaccatc
     4801 ctgcttcccc gaggctacgc cgcttcccta ccgggcgccg tgaaggcggc ggcggagcgg
     4861 ctgcgggcgg gctgcccggt gggggtgttt ccggaaggga caacgacctt cggacccggc
     4921 tgtctgccct tcaagcccgc attcttccag gcggccctgg ataccggggt gccagtccag
     4981 cccttcgccc tgacctacga gaccccggtt ccctggccgt ccgcgccccg gtccgtgcac
     5041 tgggtggact ggacccccct gccgctgcac gttttccggg tcctctgcct cccccgcata
     5101 gaggcccggg tggcctggct tcctgcgatc cttcccgagg ccggggagga ccggaggggt
     5161 ctggcgtccc gtaccgaggc ggcggtgcgc gtgggattgg cggccggggc tgcctgaggg
     5221 ccggacttcc ccctcagttg tcccgggcga cctcccgctc gtagaggtcc tggaagtagg
     5281 cccgatgacg ggattcctcc ccggccaggc gttcgaaggt ctcccggatc ccgggatccc
     5341 cggcctcccg ggccagccgg ccgtagagtt cgaagctccg ggcctccttg gaagccgccg
     5401 cggtcaggat gtcctggaag gtcatgcccg cgggggggcg gtccgtcgcc tcgactcgcc
     5461 gcgccgcgcc caggtccacc gccgtggagg cggagagctt gaccgtcccg cgggccttca
     5521 tcgcctccag catggccgcg tggcctctct ccatggcgat gaactccgcc agggtggact
     5581 tctgggccgc gaaggccgcg atgccggaca gctcccggta gaagtccacg gccgcgttct
     5641 cccggccgat ggcgaactcg agaacctccc cgaacgtggc gtatcccatg tcagtccccc
     5701 ctcccgccca ggatgcggtc gatggcccgg gccgcgcggt ggccgtccgc gatggcggag
     5761 atggcgtcct tcttttcgtt caccaggtcg ccgcccgcga agaccgtcgg gacgctcgtc
     5821 cggccggtcc ggtccgtgac gggcttcatg cggcggacct ccacgccgtc cgcctccgtg
     5881 atgtacgaca gttcggggcc ctggccgatg gccgcgatga tggagtcgta gcgctcggtg
     5941 acgaccgccc cggggatggg ttccggcacg gggcgcttgc ccggctcctt ctggaccatc
     6001 tcggcctcgt tccacacgaa gcgggcccct ccgtccccgg agtcctccac ccgcaggggg
     6061 atggcccgct cgataaggcg ggcgccctcg gccttggctt cccggatctc ctcggggtcc
     6121 gcgggcatgt cgacctggcg gcggcggtag acgatgtcca cctcgcagcc ccggcgcagg
     6181 gcggtccggg cggcgtccat agccacgttg ccgcccccga ccacggccac ccgccggccc
     6241 agtccgagct cctcgccccg ggtggcggct gccaggacgg ccaccccgtc caggacccgg
     6301 gggtgctcgt ccccgggcac gtccaggcgg tacgccgagg gcaggcccgt ggagaggtag
     6361 accgcgtcga aatcctcccg gagcttcgcc agggccggat ccttcccgac cgtcgctccg
     6421 tggcggatct ccacgcccag gctccggatg tagtccacgt ccttgtccag ggcggggtag
     6481 ggcaggcggt attccgggat cccgtaccgg agcatcccgc ccggcgcctc gtccttctcg
     6541 aagaccgtga ccttgtggcc caaaagcgcc aggtagtagg cagcggaaag tccgccgggg
     6601 ccggcgccga ccacggcgac ctttcggccc gtggcgctct ccgcccgggg aagggacttg
     6661 ccgtagtcgg ccagttccac ctggtccgcg atgtagcgct tgagccagcg gatggccacg
     6721 gggtcgcccc cggagccgat cgcgcaggcg gactcgcagg cccgggtgca gatccggccg
     6781 caggcctcgg ggaaggggtt ggtctcgtac aggaggcgca gccccgtctc aaggtcgccg
     6841 ccccggaccg cgtttatgta gccggggatg tccatgtggg ccgggcagga ggccacgcag
     6901 actccgcagg ccacgcagcg gtccgcctcc cgctcggctt gctcccggga atagcccttc
     6961 acgagctcca ggaaggactt caccccctcg gcgtgactta gttccggcat ggggacccgc
     7021 tccatgtcca ggagccggta gccctcggcg cggcggtacc cgagctctgc gccgtcccag
     7081 ggtttgggat ccaccccggg cacgtagcgg aagacctccg gatccgagtc gatccacagg
     7141 tattcgttgc tcatccccag ggagcccgag gggcagatgt ccacgcagag ggcgcaccag
     7201 cagcagcggc cgtagtccac ccggggccgg aggccggaat ccccgccgga gggcgatcgt
     7261 ccctccacgg gcaccaggtc gatggccttg ttcatgcata tgctctcgca ggtgccgcag
     7321 cccacgcacg tgtccgcgtc gttgatgtgg aagccccggt accggggcgc gccgggacgc
     7381 tcgtcgatgg gcttgcggac cgtgaagggc ttctcgaagg cgcgcttcca gacggcgaag
     7441 ggggctatga tgtccttgat atccatgggc tcacctctcg atctcgggcg gataggtgtg
     7501 gaggctcacc atgaggcccg ccacgtcggc gatgttcacg ccccggacca ggcgctccat
     7561 gacggaaacc gcgtgggtgt agctggggcc ccggaccgcg acccggcggg gcttgtcgcc
     7621 cccgtcggac accacgtaga agccgtactc accccgggtg cactccgcgg ccacgtaggt
     7681 ctcccccgcg gggatcttcc agtgcagggg gttgggcagc cgctcccgga aggggccttc
     7741 cgagggcagg aagtccacga tctggcggat caggtcgatc ccctggaaca tctcggccag
     7801 ccggagccgg gcccgttcga aggcgtccga ctccgccccg accacgggct caaaggagag
     7861 gcgggggtag gccaggtagc cccggtcctt ccggaggtcc cgcttgaagc ccgcggcccg
     7921 ggcgttcggg cccgtgatgc cgtagcggtc cacccagtcc ggggggatgt accccaggcc
     7981 gacggcccgc atcttcatca ccgcgttgtg gaacatcacg agctcgatgt ccttgaggtt
     8041 ctgctcgacg acggtcatgg tctccagcac gtgccggagg aagcccggcg ggacgtccgc
     8101 ccggaccccg ccggggatga tgtacatgtg gtagatgcga cctcccgaga gctcctcgaa
     8161 gaggtccagg acgtagtccc gcatcgtgac gctccactgg gcgatggtcc cgtgaccgaa
     8221 ggccccggcc tggccgccga tccacatgag gaagctggcc aggcggatca tctccaggga
     8281 gagggtccgg agccagaccg ccttgtccgg gacttcgatc cccgcgagct cctccagggc
     8341 ggcggcgtag cagtactcgt tgaagtccgg ctcgggcacc gcgatgcggc
//
LOCUS       DAOTEI010000154         8380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6007_length_8380_cov_4.536937, whole genome shotgun sequence.
ACCESSION   DAOTEI010000154 DAOTEI010000000
VERSION     DAOTEI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_8380_cov_4.536937"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="P5313_13085"
     CDS             <1..529
                     /locus_tag="P5313_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRY81348.1"
                     /translation="KEGIGIETGLMTTIHSYTATQKTVDGPSKKDWRGGRAAAINIIP
                     SSTGAAKAVGEVLPSTKGKLTGMSFRVPTADVSVVDLTFRATRDSSIEEIDGLLKKAA
                     GSYLKGILGYTDEELVSTDFIHDPRSSIYDSLATLQNNLPGEKRFFKVVSWYDNEWGY
                     SNRVVDMLKYMAGMK"
     gene            585..1847
                     /locus_tag="P5313_13090"
     CDS             585..1847
                     /locus_tag="P5313_13090"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRY81349.1"
                     /translation="MIKTIKDIELTGKRIVMRVDFNVPMKDGKVQDDTRITASLPTIR
                     YCLEKGVKSLVLMSHLGDPKKDSKKAKEKAEKDGKPWDEEKYLAGKHRMRPVAEHFSK
                     VLGKPVVFAGEDSCYGKKAFIESQPAGTVVMLENTRFHKEETSEDPAERDKLARELAA
                     YGEIFVNDAFGTAHRDHASTASVSKFLPVSVGGFLMAKEVEYLEPMVVNPPKPMVAVI
                     GGAKVSSKIAVLESLLKNAYALVIGGGMAYTFLRALGKKTGKSLVEEDMIPTARSILD
                     AASSAGVEIVLPLDHVCAAVFDASAAPVAVDGLDIPDDLMGMDVGPKTLSKYAKVLAS
                     AKSIVWNGPVGVFEFEAFSKGTGEVAKLVAQATGRGAVTVVGGGDSVAAVNKFGLADK
                     MSHVSTGGGASLELLEGKKLPGIEVCRK"
     gene            1863..2612
                     /gene="tpiA"
                     /locus_tag="P5313_13095"
     CDS             1863..2612
                     /gene="tpiA"
                     /locus_tag="P5313_13095"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRY81350.1"
                     /translation="MRSYYVAGNWKMHKTVGESVALAKALAAALADCGEKVMIAPAFT
                     SLAAVAEALRGSKLILGAQNMAVEEQGAHTGEVSVLMLKDLGVRAVILGHSERRHTYG
                     ESDALVNRKVRLALAHGLEPILCVGETLEEREAGRLEEVVGTQLREGLAGVSPEDFKR
                     VTIAYEPVWAIGTGKTATPEDADSVHAFCRKVLAELYGPSAAKSACIQYGGSVKPENA
                     AALMAKPNIDGALVGGASLKPESFVPIAKFR"
     gene            2730..3134
                     /gene="secG"
                     /locus_tag="P5313_13100"
     CDS             2730..3134
                     /gene="secG"
                     /locus_tag="P5313_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRY81351.1"
                     /translation="MGLLSIILLVFFIIVSVLLVLLVIVQDSDGDDLGGIFSGGSSSA
                     FGSRATNVVVRVTYVLGGLFFVFAFSLALLNRTPIGSVEAAALRRAGQTTTEWWSAES
                     QAEAPLPGSEGAQGQVTPPQDSPVPPSQNSGN"
     gene            3245..4048
                     /locus_tag="P5313_13105"
     CDS             3245..4048
                     /locus_tag="P5313_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRY81352.1"
                     /translation="MGDEPLTSYYEFLGVRPEASTREIKSAFRKKAKVFHPDTARSDD
                     RSMRFLLEAYRTLSDPLRRREYDRKLRRFEARAREVPSFEYRTWLLERREDPQYRAKL
                     VMYDLLHDRDDEALEYYESISGDERTRLVRYFERSEAMDAEFCIAELYEKRGEWRKAY
                     EVYRSLIGMEREKPAFGYFFDVVELQFRRLVLEGIPARDDPEEYLGILEESARIAVDT
                     EDAARFLRRKAEILAKAGRRGDALAALREAEFLAPRLPGIKPLLRKLGA"
     gene            4194..5231
                     /locus_tag="P5313_13110"
     CDS             4194..5231
                     /locus_tag="P5313_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRY81353.1"
                     /translation="MRRSLVSLCLLLAVVALQAQTTIDTPVATVRLTRQEVISQRSLK
                     TDFDRLETVAKRKLTPEERRQVLDMRINNALFFQFCEREKITATDAELNAQISNMRSQ
                     LGPSATDANLEQVLRSRGITLDLKVWARQQVLLQKYIQTKQADRLKAIAQPTADEIIK
                     EYEFNKKDYFLPEMARVSMIYVDLKDKGAEDRRKALETLNGLSSQIKANPEKFGEFVT
                     RAMAGGMPYKASSQFTLPKTADAVRAFGQKFTDAAFTVKPGRVSDPIESAGVQLTGYA
                     ILRVEEQSPEKFLGLADLIPGQNRTVQQLIQYKLGMERLQNTLDQIENDLLKQLRSEA
                     TIKIVDEKLEF"
     gene            5234..5659
                     /gene="nusB"
                     /locus_tag="P5313_13115"
     CDS             5234..5659
                     /gene="nusB"
                     /locus_tag="P5313_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRY81354.1"
                     /translation="MASRRKGRILAFQALYAWEASRQPLEDLLSYEWVEETKREALED
                     ELRAFAGLLISGTIENLEAVDACIQRHLEHWTLERLKKVDLAVLRLSAYSLMYQKDIP
                     AQITIDEAIEIVKEYGSEDSYKFVNGVLDAIWKAESKAS"
     gene            5625..7181
                     /locus_tag="P5313_13120"
     CDS             5625..7181
                     /locus_tag="P5313_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81355.1"
                     /translation="MPSGKRSPRLPELPRAALRTLAPLPALAALAALLGSCYPGAGFE
                     RTLRRLDAGDHDAARASRLLDAGARRARSSSDWLRLLSRAFLVSERTGNPSLAALTAD
                     RARKAYPTYEDIALAACRAYIDAGRPGAALSLFPAPLDPALRPSWFAEAFLEEYRRSG
                     RTSASADEDVLLRIAEASGRGEPAVNAALLRMRAGDREGAAWFLRRGIELGAAPDPDL
                     AWDAGVLSSILSDPERALGGRDLERRADAALLLGERDWAREYLTELVLADPAYSWKAY
                     ASLAALEDDLERADYWYDRMASEFPSDPEAFRARAAHLARTGREGRALEGLESEPGGR
                     SDPRTAVLAAEIGFRIKPGEPRAAKAIELANTFPAEPYVQTWALGTLAAAERFAEAAE
                     GYRQVKSRGMDLGRPWYLEALSLILEGRSAEAAQLIERDGPAEAGPEAPFALGVLYSE
                     SGDHARAAERFRIAASAARDAETRARALTEAGKEYAALGERRNARDAWAAALAVKPDH
                     AEALRLLGSR"
     gene            7204..7950
                     /gene="map"
                     /locus_tag="P5313_13125"
     CDS             7204..7950
                     /gene="map"
                     /locus_tag="P5313_13125"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRY81356.1"
                     /translation="MLKLKNEEQIRGIRESCRLLSGLFAELRPLVREGVSTRDLDEYT
                     RTFILKAGGRPAFLGYNGYPASLCVSVNEEVIHGIPGPRKVKSGDIVGLDCGIDLGGY
                     FSDAAITVPVGKVSAEIEALLRTTRECLEKALAAIRVGGRIHDISRAVYGHARSRGYG
                     IVRQWCGHGVGFSPHEDPQVPNYVGAGPNPRIVPGMVLAIEPMINLGGDDVETLDDDW
                     TVVTADGKPSAHFEHTVAILPEGPEVLTSW"
     gene            8030..>8380
                     /locus_tag="P5313_13130"
     CDS             8030..>8380
                     /locus_tag="P5313_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRY81357.1"
                     /translation="MSLRDKLSSKNFFLVNLVLLGILIGFSAAFVSLTASARARAPAV
                     VYAEGPGSGTVAEDAALASAESLQTAFNRVAESVLTSVVELDVVETVTSQAPSPDQFP
                     WRFFFGPQEDGQNPQ"
BASE COUNT         1463 a         2810 c         2773 g         1334 t
ORIGIN      
        1 caaggagggg atcgggatcg agacgggcct catgaccacc atccacagct acactgccac
       61 ccagaagacc gtggacggcc cctccaagaa ggactggcgg ggcggccgcg ccgcggcgat
      121 caacatcatc ccttccagca ccggcgccgc caaggccgtt ggggaggtcc ttccctccac
      181 caagggcaag ctcacgggca tgtccttccg ggtccccacc gcggacgttt ccgtcgtgga
      241 cctgaccttc cgggcgaccc gggattcctc catcgaggag atcgacggcc tgctcaagaa
      301 ggccgcgggt tcctacctca agggcatcct ggggtacacg gacgaggagc tcgtctccac
      361 ggacttcatc cacgatcccc gctcctccat ctacgacagc ctggccaccc tccagaacaa
      421 cctccccggg gagaagcggt tcttcaaggt cgtcagctgg tacgacaacg agtggggcta
      481 ttccaaccgc gtcgtcgaca tgctcaagta catggcgggc atgaagtaag atcccggggc
      541 gcgggccgga acggcaccgc gccccgctca ttccggagaa ccccatgatc aagaccatca
      601 aggatatcga gctgacgggc aagcggatcg tcatgcgcgt ggacttcaac gtccccatga
      661 aggacgggaa ggtccaggac gacacccgga tcaccgcctc cctgcccacg atccggtact
      721 gcctggagaa gggcgtgaaa agtctggtcc tcatgagtca cctcggagat ccgaagaagg
      781 actccaagaa ggccaaggag aaggcggaaa aggacggcaa gccctgggac gaggagaagt
      841 acctggcggg caagcaccgg atgcgccccg tggcggagca cttctcgaag gtcctcggca
      901 agcccgtggt cttcgcggga gaggattcct gctacggcaa gaaggccttc atcgagtccc
      961 agcccgccgg aacggtggtc atgctggaga acacccggtt ccacaaggag gagaccagcg
     1021 aggacccggc cgagcgggac aagctggccc gggaactggc cgcctacgga gagatcttcg
     1081 tgaacgacgc cttcggcacg gcccaccggg accacgcctc cacggccagc gtctccaaat
     1141 tcctgcccgt gtccgtgggg ggcttcctga tggccaagga ggtcgagtac ctggaaccca
     1201 tggtcgtgaa tccgcccaag cccatggttg cggtcatcgg gggggccaag gtttcctcca
     1261 agatcgcggt cctggaaagc ctgctcaaga acgcctacgc cctggtcatc gggggcggga
     1321 tggcctacac cttcctccgc gccctgggca agaagaccgg gaagtccctg gtggaggagg
     1381 acatgatccc caccgcccgg tccatcctgg acgccgcgtc ctccgcgggg gtcgagatcg
     1441 tcctgcccct ggaccacgtc tgcgccgcgg tcttcgacgc ctcggcggcc ccggtggccg
     1501 tggacggcct ggacatcccg gacgacctga tgggcatgga cgtcggcccc aagaccctgt
     1561 ccaagtatgc caaggtcctg gcctccgcca agagcatcgt atggaatggg ccggtcgggg
     1621 tcttcgagtt cgaggccttc tccaaaggca ccggcgaggt ggccaagctc gtggcccagg
     1681 ccacgggacg cggagccgtg accgtggtcg gcggggggga ttccgtggcg gcggtcaaca
     1741 agttcggcct ggccgacaag atgagccacg tgtccacggg gggcggcgcc agcctcgaac
     1801 tcctggaggg caagaagctc ccgggcatcg aggtctgcag aaaataagat aaggagagct
     1861 caatgcgttc ctattacgtc gcgggcaatt ggaagatgca caagaccgtc ggagagtccg
     1921 tggccctggc caaggccctg gccgccgccc tggcggactg cggggagaag gtcatgatcg
     1981 ccccggcctt cacctccctg gccgccgtgg ccgaggccct ccggggttcg aagctgatcc
     2041 tgggggccca gaacatggcc gtggaggagc agggcgccca caccggggag gtttccgtcc
     2101 tgatgctcaa ggacctcggg gtccgggccg tgatcctggg ccattccgag cgtcgccata
     2161 cctatgggga atccgacgcc ttggtgaatc gcaaggtcag gctggccctg gcccacggcc
     2221 tggagccgat cctctgcgtc ggggagaccc tggaagaacg ggaggcgggc cgtctggaag
     2281 aggtcgtggg aacccagctg agggagggcc tggcgggagt ctcccccgag gacttcaagc
     2341 gggtcaccat cgcctacgag cccgtctggg ccatcgggac cgggaagacc gccacccccg
     2401 aggacgcgga ttccgtgcac gccttctgcc gaaaggtcct cgcggagctc tacggaccgt
     2461 ccgccgccaa gtccgcctgc atccagtacg gcggatcggt caaaccggag aacgccgcgg
     2521 ccctcatggc caagccgaat atcgacggtg ccctggtggg aggcgcgagc ctcaagcccg
     2581 aatccttcgt acccatcgcc aagttccggt agggcagggg cttgcctatt gatcctgcgg
     2641 gcggtacgcg ttattatgcc ttgccccgaa ggggcgcgcg gcgggcggta tgacccgcgg
     2701 ccgcggaatc cgtcgacgga gtctaatcca tgggtctttt aagcatcatc cttctggtct
     2761 tcttcatcat cgtctcggtc ctcctggtgc tccttgtgat cgtccaggat tcggacggcg
     2821 atgacctggg cggcatcttc tccgggggaa gctcctccgc gttcggatcc cgggccacca
     2881 acgtggtggt gcgggtcacc tacgtcctgg gcggcctctt cttcgtcttc gccttcagcc
     2941 tggccctcct gaaccggacc ccgatcggct ccgtggaggc cgcggcgctc cgccgggcgg
     3001 gccagaccac gacggagtgg tggtccgcgg aaagccaggc ggaagccccc ctgcccggtt
     3061 ccgaaggagc ccagggccag gtaacccccc cgcaggattc gccggtcccg ccctcccaga
     3121 actccgggaa ctgatctcgg ggctgtcccg cgggccgcga gcccccggga cagccgcatc
     3181 ccgtccgatc cgcatcggtc ccgcttcgtt ccgcgcccgc ataccgtcct ttccggagcc
     3241 atcgttggga gacgaaccgc ttacctcgta ctacgaattc ctgggcgtcc gccccgaggc
     3301 ctccacccgc gagatcaaga gcgcgttccg gaaaaaggca aaggtcttcc atcccgacac
     3361 cgcgcgcagc gacgatcgct ccatgcgctt cctgctggag gcgtaccgga ccctctcgga
     3421 tcccctccgg cgccgcgagt acgaccgcaa gctccggcgt ttcgaggcga gggcgcggga
     3481 ggttccctcc ttcgagtaca ggacctggct cctggagcgc cgggaggatc cccagtaccg
     3541 ggccaagctc gtgatgtacg acctgctcca cgaccgggac gacgaggccc tggagtacta
     3601 cgagtccatc tccggcgacg agcgcacccg gctggtccgg tatttcgagc ggtcggaggc
     3661 catggacgcc gaattctgca tcgccgagct ttacgaaaag cggggcgagt ggaggaaggc
     3721 ctacgaggta taccggagcc tgatcggcat ggagagggaa aagcccgcct tcggatactt
     3781 cttcgacgtg gtggagctcc agttccgccg gctggtcctg gagggaatcc ccgcccggga
     3841 cgatccggag gaatacctgg gcatcctgga ggaatccgcc cggatcgccg tggacaccga
     3901 ggacgccgcc cgcttcctcc gcaggaaggc ggagatcctg gccaaggccg gccgccgggg
     3961 ggacgccctg gccgccctga gggaggcgga gttcctggcc ccccggctgc cgggcatcaa
     4021 gcccctgctg cgcaagctgg gcgcttgagg acccacgagc cttgaacaaa cccggtgttt
     4081 tgtttacctt tcatcggatc gtttgttcta actacgtgta cggccgtccg agaagcgcac
     4141 ggcttgtcgg gactgcctgc tcaaggatcg attccattcg acaaggaaac cctatgcgtc
     4201 gcagccttgt atccctttgc ctcctcctcg ccgtcgtcgc ccttcaagcc cagacgacca
     4261 tcgacacccc cgtggccacg gtgcgcctca cccggcagga ggtgatctcc cagcgcagcc
     4321 tcaagacgga tttcgaccgg ctggagaccg tggccaagcg caagctcacc cccgaggagc
     4381 gcaggcaggt cctggacatg cgcatcaaca acgccctctt cttccagttc tgcgagaggg
     4441 agaagatcac cgcgacggac gccgagctca acgcccagat ctcgaacatg cgttcccagc
     4501 tggggccctc cgccacggac gcgaacctgg agcaggtcct ccggtcccgg gggataaccc
     4561 tggacctcaa ggtctgggcc cgccagcagg tactcctgca aaaatacatc cagacgaagc
     4621 aggcggaccg cctgaaggcc atcgcccagc cgacggcgga cgagatcatc aaggaatacg
     4681 agttcaacaa gaaggactac ttccttccgg agatggcccg ggtgtccatg atctacgtgg
     4741 atctgaagga caagggcgcg gaggaccgca ggaaggccct ggagaccctg aacggcctgt
     4801 cttcccagat caaggcgaat cccgagaagt tcggggagtt cgtgacccgc gccatggcgg
     4861 gagggatgcc ctacaaggcc tcgtcccagt tcacccttcc caagaccgcg gacgcggtcc
     4921 gcgccttcgg ccagaagttc acggacgcgg ccttcaccgt caagcccggc cgcgtcagcg
     4981 acccgatcga gagcgccggc gtccagctga cagggtacgc catcctccgg gtggaggagc
     5041 agagccccga gaaattccta ggtctggcgg acctgatccc gggccagaac cggacggtcc
     5101 agcagctcat ccagtacaag ctgggcatgg aacggctcca gaacaccctg gaccagatcg
     5161 agaacgacct gctcaagcag ctgagatccg aggcgaccat caagatcgtc gacgaaaagc
     5221 tggagttcta ggcatggcgt cccgcaggaa ggggaggatc ctcgccttcc aggcgctcta
     5281 cgcgtgggag gcttccaggc agcccctgga ggacctgttg tcctacgagt gggtggagga
     5341 aaccaagcgg gaagccctgg aggacgagct ccgggccttc gcgggtctcc tgatctcggg
     5401 cacgatcgaa aacctcgagg ccgtcgacgc ctgcattcaa cggcatctgg agcactggac
     5461 cctggagcgg ctcaagaagg tggatctcgc ggtgctccgg ctttccgcgt attccttgat
     5521 gtaccagaag gacatacccg cgcagatcac gatcgacgaa gccatcgaga tcgtgaagga
     5581 atacggatcc gaggattcct acaaattcgt gaacggggta ctggatgcca tctggaaagc
     5641 ggagtccaag gcttcctgaa ctccctcggg cggcgctcag gaccctggcg ccgcttccgg
     5701 ccctggcggc cctggccgcc ctgctgggtt cctgctatcc cggtgccgga ttcgaacgga
     5761 ccctccgtcg cctggacgcc ggggatcacg acgcggcccg ggcttcccgg ctgctggacg
     5821 cgggagcccg gcgggcgcgg agctcctcgg actggctgcg cctcctgtcc cgggccttcc
     5881 tcgtctcgga acgcacggga aatccttcct tggccgccct gacagcggac agggcccgga
     5941 aggcctatcc cacctacgag gacatcgccc tggcggcctg ccgggcgtat atcgacgcgg
     6001 gccgccccgg ggccgcgctg tccctctttc cggctcccct ggatcccgcg ctccggccgt
     6061 cctggttcgc cgaggccttc ctggaggagt accgccgttc cggccggact tccgcctcgg
     6121 cggacgagga cgtcctcctt cggatcgcag aggcttcggg caggggggag cccgccgtga
     6181 acgccgccct cctccggatg cgcgcgggag accgggaagg cgctgcctgg ttcctccgca
     6241 ggggtatcga actcggcgcc gccccggatc cggacctggc ctgggacgcc ggcgtcctgt
     6301 cctcgatcct gtcggatccc gagagggcgt tagggggccg ggacctggag cgccgcgcgg
     6361 acgcggccct gctcctggga gagcgggact gggcccggga atacctgacg gagctggtcc
     6421 tggcggaccc ggcctattcc tggaaggcct acgcttccct cgcggccctg gaggacgacc
     6481 tcgaacgggc cgactactgg tacgaccgca tggcctcgga gtttccctcc gatcccgagg
     6541 cattccgagc ccgggccgcc cacctggccc ggacgggacg ggagggtcgg gccctggagg
     6601 gactggaatc cgagccgggg gggaggtcgg atccccggac cgccgtcctc gccgcggaga
     6661 tcggcttccg gatcaagccc ggggagcccc gggcggcgaa ggcgatcgaa ctggccaaca
     6721 ccttcccggc ggagccctac gttcagacct gggccctggg taccctcgcg gccgcggaac
     6781 ggttcgcgga agccgccgag gggtatcgcc aggtgaaatc ccggggcatg gacctgggcc
     6841 gaccctggta cctggaagct ctgtccctca tactggaagg ccgctccgcc gaggccgccc
     6901 agctcatcga gcgggacggc ccggccgagg ccggtccgga ggcccccttc gccctggggg
     6961 tgctctattc cgagtccggc gaccatgccc gggcggcgga gcggtttcgg atagccgcgt
     7021 ccgcggccag ggacgcggag acccgggcga gggcgctgac cgaggccggg aaagaatacg
     7081 cggccctggg ggaaaggcgg aacgcccggg acgcctgggc cgccgccctg gccgtcaagc
     7141 cggatcacgc ggaagccctg aggctcctgg gctcccgata gtcgcgttgg gaaagaggtt
     7201 ttcatgctga agctcaagaa cgaggaacag attcggggga tccgggaatc ctgccgcctc
     7261 ctttccggtt tattcgcgga gctccgtccc ctggtccggg aaggggtctc gacacgggac
     7321 ctggacgaat atacccggac cttcatcctg aaggccgggg ggcgccccgc cttcctggga
     7381 tacaacgggt atcccgcctc cctgtgcgtg tccgtcaacg aggaggtcat ccacggaatc
     7441 cccgggccga ggaaggtcaa gagcggcgac atcgtggggc tggactgcgg gatcgacctc
     7501 ggggggtatt tttcggacgc cgccatcacg gttcccgtgg gcaaggtgag cgcggagatc
     7561 gaagcccttc tccggacgac ccgggaatgc ctggaaaagg ccctggccgc gatccgggtg
     7621 gggggacgca tccacgacat ctcccgggcg gtctacggtc acgcccgaag ccggggctac
     7681 ggcatcgtcc ggcagtggtg cggccacggg gtggggttct ccccccatga ggatccccag
     7741 gtgcccaatt acgtcggcgc ggggccgaat ccccggatcg tccccggcat ggtcctggcc
     7801 atagagccca tgatcaacct gggcggcgac gacgtggaga ccctggatga cgactggacc
     7861 gtggtcaccg cggacgggaa gccctcggcc catttcgagc ataccgtggc catccttccg
     7921 gagggacccg aggtcctgac gtcctggtag gggagctccc tgtaaccatt ccggccgctt
     7981 gttagtatat tgtatcgaac gcggacgagt cgctcgcaag gagtatgcca tgtcgcttcg
     8041 agacaagttg agctccaaga atttctttct cgtcaacctt gttttattgg gtatcctgat
     8101 aggtttttcc gcggccttcg tatccctgac ggcttccgcc cgcgcgcggg cccctgcggt
     8161 cgtctacgcg gaaggccccg gttcggggac ggtcgcggag gatgcggccc tcgccagcgc
     8221 tgaatccctc cagaccgcgt tcaaccgggt ggcggagtcg gtcctgacct ccgtggtgga
     8281 actcgatgtc gtggagaccg tgacctccca ggccccgagt cccgaccagt tcccctggag
     8341 attcttcttc gggccccagg aggacggcca aaacccccag
//
LOCUS       DAOTEI010000155         8312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6071_length_8312_cov_5.130798, whole genome shotgun sequence.
ACCESSION   DAOTEI010000155 DAOTEI010000000
VERSION     DAOTEI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6071_length_8312_cov_5.130798"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1617
                     /locus_tag="P5313_13135"
     CDS             280..1617
                     /locus_tag="P5313_13135"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRY81358.1"
                     /translation="MPSLGTRWASAGRRFLFTAELYGKRELANHAAAGAYAFLLSATP
                     AFLVLLAASSWFLRGSEAAAGILKSVLGEILGPHTLEGIARAYLSRPLTGFAAVFGIV
                     NLVWASRLLVASIQRGLRTIWADTGPVRPVRENLLTFAVELVLLVSVIAVLAASQAAR
                     IVLDSVRNLAARGTLLRFYRAAYLAAPHAALFLFSYGTYRTIPPARPRHSHCLAGAAL
                     CAAVYALLRALLDSIRNIARYDLLYGVLGNLILGLINVYLFFSLYFFFAGFVYVLESF
                     DALLLGRYLHRKRPRSTEAPASDPEAGMPYRLLRRFGRDYPEGAKIFRAGDEGNEVFL
                     LREGEVGILQAPAAGGPPVQVSTVRAGEFFGEMAYLLGDRRTATTVALTPVSVLALPV
                     RLFERFLAVDARASRRLVKLLSARLSETTRLAAEAQARDALVLDDGDDRGDSP"
     gene            1614..2147
                     /locus_tag="P5313_13140"
     CDS             1614..2147
                     /locus_tag="P5313_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HRY81359.1"
                     /translation="MKILVVSDTHGDLGNLERVLRILGSRLDLLLHLGDGARDPDRLP
                     RSSERSIPCLQVKGNMDSDPHLPPSRVTRAGGHLLYLSHGHAALASGSPLPMILAARE
                     AGASACLFGHTHVPHKSYSEGLLVLNPGSLSRPRNGWGPSFALLTVPEVGDGRIEATH
                     YEIRDNPDGPAFRVFDP"
     gene            2211..2852
                     /locus_tag="P5313_13145"
     CDS             2211..2852
                     /locus_tag="P5313_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81360.1"
                     /translation="MKKTTAILAGALLLALGASAGLYLALRAIPRKGVEREFLSYVEG
                     LSETGHLILVRGRERLTVRESAKGQLFGDTTLGDLLRIRSDAVVELSAWAELSWAVDL
                     ADPERWSVRLNRKERVLHVASPPLRTLTPALRTETLEARVVNRSVFLDEKRMIDSVRA
                     ALTGRFEEIARTTSEEPEVRGRAAASLSAIALAFCKKFGLPADRVEVVFGLEE"
     gene            complement(3061..3762)
                     /locus_tag="P5313_13150"
     CDS             complement(3061..3762)
                     /locus_tag="P5313_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRY81361.1"
                     /translation="MAASRVLVVGAGGLGSPVLYYLAAAGVGSLGIADRDTVSLTNLN
                     RQILHNPGDLGRAKTESARDKLAAFRPDLELRLHPGSLDDGNAPGIVAGYDLVLAAVD
                     SRESRRVVNRACYARGVPWIDGGVDGFEGLVSVYRPPSGPCYECLTRGIPDDPGHVPR
                     LFGALAGTVGLLQVQEALKLLLGIGRPLAGRVLFYDSREPRFDVVEVPADPSCPVCAN
                     ARSPGINHGDHGEHG"
     gene            complement(3867..4418)
                     /locus_tag="P5313_13155"
     CDS             complement(3867..4418)
                     /locus_tag="P5313_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HRY81362.1"
                     /translation="MIRIRTPLDDGTARSLRAGDEVRISGTVYAARDAAHARLLVLIR
                     DGKPLPLDLRGAVIYYVGPTPPSEGMVCGSAGPTTSGRMDGTTPALLDRGLKGMIGKG
                     FRSPEVIEAMKRNGAVYFAATGGAGALLARHIRALEVVAWPELGPESLKRLEVEEFPA
                     VVAVDCQGGNLYEEGPARYRRDA"
     gene            complement(4415..5260)
                     /locus_tag="P5313_13160"
     CDS             complement(4415..5260)
                     /locus_tag="P5313_13160"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRY81363.1"
                     /translation="MRRIDCSEVREAVKAELVRINREIGPDVLSALEAGLAAEESPVG
                     RAVLDQLIENDRLAAREGRAICQDTGMVVAFVTLGQEVHLTGGSFREAVDEAVRGAYR
                     DARLRNSVVRDPLFDRSNTGDNTPAVVHLDLVPGDGLSIELMVKGFGSENMSALAMLN
                     PADGEAGVRRLVVDTVRRAGPNPCPPVIVGVGLGGTFERAALLSKKALLRPLGEPSPD
                     PRYAALERSLLADLNRLGIGPAGLGGLVTSLAVHVETCPTHIASIPAAVNVSCHASRH
                     GRVIL"
     gene            complement(5269..5445)
                     /locus_tag="P5313_13165"
     CDS             complement(5269..5445)
                     /locus_tag="P5313_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81364.1"
                     /translation="MKTKIRLPLVSAALMALLLAAGCVSAPKEAETVPAAQTEADAGS
                     SASTDAESGATASN"
     gene            5676..6455
                     /locus_tag="P5313_13170"
     CDS             5676..6455
                     /locus_tag="P5313_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRY81365.1"
                     /translation="MRREKTTLLRKLSMYGFDLVTGSHNFFALLEFDVTDLRRHLRAL
                     RAEGRGGSLFAFLLKAVGKCLEEFPEFNAMIDRRKTTYFDEVDVNIPIEIETEGIRNP
                     KQYIVRDINRKSLTEVDREIAASKDAGDDHTGYVFSKGLQRLLGILPAGLVMFLMRSI
                     LRRHDTVQKLSGTVFVTSVSMFSNVPGYVIPYAGGPKAASFAIGSVSKKPVVRGDEVR
                     VREMINLTAIFNHDLIDGAPAARFINRLRRYVETSFAELTG"
     gene            6452..7063
                     /locus_tag="P5313_13175"
     CDS             6452..7063
                     /locus_tag="P5313_13175"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HRY81366.1"
                     /translation="MSAAPPSARILLLTGDRGAGKSTLCELLAGEAEGRGIPAGGIAC
                     PAVFGPDGTKIGCRVRDLAAGEERDLGSTALDLGGPRWKGWSFSEEGFAAANRAVLAA
                     LDRGAGLVILDEIGPVELRLGAGLEPSLRALDERYAGPDLPGPGGRRDVAAGPLVLLV
                     VRPELAEELGRRFPGSAIIRVDPGDRRAALDLARDALFRAPRP"
     gene            complement(7094..7765)
                     /locus_tag="P5313_13180"
     CDS             complement(7094..7765)
                     /locus_tag="P5313_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRY81367.1"
                     /translation="MSDILLPYRSSHERRYSPGEEIANGVTHGAGALLSVAALVILVV
                     FAVLRGSARHVVSYAVFGSSLVVLYTMSALYHSVTNPRVKRILEILDHSSIYVLIAGT
                     YTAFALTALPPALGWWIFGAEWALALAGIALKAVWFDRWKLLSVLGYVAMGWMIAPAL
                     GHLRENLPAESVALLAAGGVAYTAGAVFYVIKRVKWFHAVWHLFVLAGSALHFFSALR
                     ALPGA"
     gene            complement(7901..8308)
                     /gene="msrB"
                     /locus_tag="P5313_13185"
     CDS             complement(7901..8308)
                     /gene="msrB"
                     /locus_tag="P5313_13185"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRY81368.1"
                     /translation="MERDLLQAVPVVARGGTEPPFQNAYWNNHAQGIYVDAVDGTPLF
                     TSKEKYDSGTGWPSFWTPISQDSLELVEDLSYGMRRIEVRSKSTGAHLGHVFDDGPAP
                     TGLRYCMNSASLRFIPREDLAQQGFPELAKLFE"
BASE COUNT         1273 a         2905 c         2806 g         1328 t
ORIGIN      
        1 cggcgacgag cttccgctcg tcgatttgcc cgaacggccg aaggccgctg cgcggcctcg
       61 tggcgacgag cttgcgctcg tcgattcaca cgaacggccg agggccgctg cgcggccctg
      121 cggcgacgag cttccgctcg tcgatttgcc cgaacggccg aaggccgctg cgcggcctcg
      181 tggcgacgag cctgcgctcg tcgatttgcc gaacggccgg aggccgctgc gcggcctcgc
      241 gggcttgcat cccgtcgggg actggggcag aatgatccca tgccctccct ggggacccga
      301 tgggcgtcgg ccgggcgccg tttcctcttc accgcggaac tctacggaaa acgggaactg
      361 gcgaaccacg cggccgcggg agcctacgcc ttcctcctgt ccgccacccc cgccttcctg
      421 gtcctcttgg ccgcatcctc ctggttcctc cggggctccg aggcagcggc gggaatcctg
      481 aagtccgtcc tgggggaaat cctggggccc cacaccctgg agggcatcgc ccgggcctac
      541 ctctcccgcc ccctcacggg cttcgcggcg gtcttcggca tcgtgaacct cgtatgggcc
      601 tccaggctcc tggtggcctc catccagagg gggctccgga ccatctgggc ggacacgggt
      661 ccggtccggc cggtccggga gaacctcttg accttcgccg tggagctggt cctcctggtc
      721 tcggtcatcg cggtgctggc ggcctcccag gcggcccgga tcgttctgga ctccgtccgg
      781 aacctggccg cccggggcac cctccttcgg ttctaccggg cggcctacct ggcggccccc
      841 cacgccgctc tcttcctgtt ctcatacggc acctaccgca cgatccctcc cgcccggccc
      901 cggcactccc actgcctggc gggagccgcg ctctgcgcgg ccgtgtacgc cctcctccgg
      961 gcccttctcg actcgatccg gaacatcgcc cggtacgacc tcctgtacgg ggtcctgggg
     1021 aacctcatcc tggggctcat caacgtgtac ctcttcttct ccctctactt cttctttgcg
     1081 ggcttcgtgt atgtcttgga atccttcgac gccctcctcc tgggccgata cctgcaccgg
     1141 aaacggccgc ggagtaccga ggccccggcc tccgacccgg aggcggggat gccctaccga
     1201 ctcctccgga ggttcggacg ggactacccg gagggggcga agatcttccg tgccggggac
     1261 gaggggaacg aggtcttcct gctccgggag ggcgaggtcg gcatcctcca ggctcccgcc
     1321 gcgggcggcc cgcccgtcca ggtgtccacg gtccgggccg gggaattctt cggcgagatg
     1381 gcctacctcc tgggggatcg gcggacggcg accaccgtag ccctgacccc ggtttccgtc
     1441 ctggccctgc ccgtccgcct cttcgagcgc ttcctggccg tggacgcccg ggcttcccgc
     1501 cgcctggtca agctcctgtc cgcccgcctt tccgagacga cccggctggc cgcggaagcc
     1561 caggcccgtg acgccctggt cctggacgac ggggatgacc ggggggattc cccatgaaga
     1621 tcctggtggt cagcgacacc cacggcgacc tgggcaacct ggaacgggtc ctccggatcc
     1681 tgggctcccg actggacctc ctgcttcacc tgggggacgg cgcccgcgac ccggaccgtc
     1741 tgccccggtc ctcggagcgt tccatcccct gccttcaggt gaaggggaac atggactccg
     1801 accctcacct cccgcccagc cgggtaaccc gggcgggggg gcacctgctg tatctctccc
     1861 acggccacgc ggccctggct tccggctccc ccctgcccat gatcctggcc gcccgggagg
     1921 ccggagcctc cgcctgcctc ttcggccaca cccacgtgcc gcacaagtcg tactcggagg
     1981 ggctcctcgt gctgaacccc ggcagcctgt cccggccccg gaacggatgg ggcccctcct
     2041 tcgccctcct gaccgtcccg gaggtcgggg acggccggat cgaggcgacg cactacgaga
     2101 tacgggacaa tccggacggg cccgccttcc gggtcttcga tccctaggag ccggaggtcc
     2161 tccggctcgc acggttgcgc gccgccccgg aacggcctat ccttggaagc atgaaaaaga
     2221 ctaccgcgat tttggcaggc gccctgctcc tggccctggg agcgtcggcc gggctctacc
     2281 tggcactgcg ggcgatcccc cggaagggcg tggagcggga attcctttcc tacgtcgagg
     2341 ggctctccga gaccgggcac ctgatcctgg tccggggtcg ggagcggctg acggtccggg
     2401 aatccgccaa gggccagctc ttcggcgaca ccaccctcgg agatctgctt agaatacgtt
     2461 ccgacgcggt cgtggaactg tccgcctggg cggaactctc ctgggccgtg gacctggccg
     2521 atcccgagcg ctggtccgtg cgcctcaacc ggaaggagag ggtccttcac gtcgcgtcgc
     2581 cgcccctgcg caccctgacc cccgcccttc gcacggagac cctggaagcc agagtcgtga
     2641 accgttccgt ttttttggac gagaagagga tgatcgactc cgtccgggcg gccctgacgg
     2701 ggcgcttcga ggagatcgcc cggaccacct cggaggagcc ggaggtgcgg ggccgcgccg
     2761 ccgcctccct gtccgcgatc gccctggctt tctgtaagaa attcggcctg ccggcggacc
     2821 gcgtggaggt cgtcttcggg ctggaagagt gaaggagatt ccaggactca ccacggagga
     2881 cacggaggtc acggggaacc tggccatgga accataggaa agacaggatt tacaagatta
     2941 gaataggatg atcaagataa aagagaagaa gtaccgaatg tcccctacac tcaaccacta
     3001 tcctgttcat ccttttttat cttgttcatc ttgtcggatt tatggttcga tgcttcgctc
     3061 ttatccgtgc tctccgtgat ctccgtggtt tattccggga gatcgcgcgt tcgcgcagac
     3121 ggggcaggag gggtccgcgg gaacctcgac cacgtcgaac cggggttccc gggaatcgta
     3181 gaacaggacc cgcccggcca ggggccggcc gatccccagg agcagcttca gggcctcctg
     3241 gacctgcagc agtccgacgg tcccggccag ggcaccgaaa agccggggaa cgtgcccggg
     3301 atcgtccgga atcccccggg tcagacattc gtagcagggc ccggaggggg gccggtagac
     3361 cgagaccaaa ccctcgaacc cgtccacccc gccgtcgatc caaggcactc cccgggcgta
     3421 gcaggcgcgg ttgaccaccc tgcgggattc tcgactgtcc acggcggcca ggaccaggtc
     3481 gtagcctgcc acgatgcccg gggcgttccc gtcgtccagg gacccgggat ggagccggag
     3541 ttccagatca gggcggaaag ccgccagctt gtcccgggcg gattccgtct tggcccggcc
     3601 cagatcgccg ggattgtgga ggatctggcg gttgaggttg gtcaggctca ccgtgtcgcg
     3661 gtccgcgatg cccagggatc cgacgccggc cgcggccagg tagtagagga cgggggaacc
     3721 caggcctccg gcccccacga ccaggaccct ggaggcggcc agccgcctct gtccctcgac
     3781 gccgaccccg ggcaggccca gttggcgctc gtaccggaca agatcttcgg ggatcccttc
     3841 gatggctcac ctcctccgcg cggttttcac gcgtcccgtc ggtaccgggc gggtccttcc
     3901 tcgtagaggt tccccccctg gcaatccacg gccaccacgg cggggaattc ctcgacctcc
     3961 agccgcttga gggactccgg tcctaactcc ggccaagcga ccacctccag ggcccggatg
     4021 tgccgagcca gcagggcccc ggcccctccg gtggccgcga agtataccgc cccgttccgc
     4081 ttcatggcct cgatcacctc cggggatcgg aagcccttgc cgatcatgcc cttcagtccc
     4141 cggtccagca gggccggggt ggtgccgtcc atgcgtccgc tggtcgtggg acccgcggac
     4201 ccgcagacca tcccctccga ggggggcgtg ggaccgacat agtagatcac cgcgccccgg
     4261 agatcgaggg gaaggggctt cccgtcccgg atcagaacca ggaggcgggc atgggccgcg
     4321 tcccgggcgg cgtacacggt cccgctgatc cggacctcgt cccccgccct cagggaccgg
     4381 gccgtcccgt cgtccagggg agtacggata cgtatcacag gatcacccta ccgtgccggg
     4441 aggcgtgaca ggagacgttg accgccgcgg ggatggaggc gatgtgggtg ggacaggtct
     4501 cgacatggac cgccagggag gtgacaaggc ctcccaggcc ggccggcccg atccccaggc
     4561 ggttcaggtc cgcaaggagg gaccgctcga gggcggcgta ccggggatcg gggctgggct
     4621 cccccagggg ccgcaggagg gccttcttgg acagcagggc cgcccgctcg aaggttccgc
     4681 cgaggcccac ccccacgatc acggggggac agggattggg ccccgcgcgc cgcaccgtgt
     4741 ccacgaccag gcggcgcacc cccgcctcgc cgtccgcggg gttcagcatg gccagggcgc
     4801 tcatgttctc gctgccgaag cccttgacca tgagctcgat ggataggccg tccccgggca
     4861 cgaggtccag gtggaccacc gcgggggtgt tgtccccggt gttggagcgg tcgaacaggg
     4921 gatcccgcac gaccgagttc cggagccggg catcccggta ggcgccgcgg acggcctcgt
     4981 cgaccgcctc ccggaaggag cccccggtca ggtggacctc ctggcccagg gtcacgaagg
     5041 cgacgaccat gcccgtgtcc tggcagatcg cccggccctc ccgggccgcg agccggtcgt
     5101 tctcgatcag ctggtccagg acagcgcggc ctacggggga ctcctcggcc gcgagccccg
     5161 cctccagggc cgagagcacg tccggtccga tctcccggtt gatccggacg agctcggcct
     5221 tcacggcttc gcggacctcg gaacagtcga tccgcctcac ggcatccctc agttcgaagc
     5281 cgtcgctccg ctttccgcgt ccgtggaggc ggaactgccg gcgtccgcct cggtctgggc
     5341 ggccggaacg gtttcggctt ccttcggggc ggagacgcag cctgcggcca ggagcagggc
     5401 catcagggcg gcggagacga gggggagtcg gatcttggtc ttcatggaat cctccgggaa
     5461 tactatagcc gaagtccgtc aacacggaaa cggccggggt gaccaatgcg tcggttacgt
     5521 attggacacc ccgtatctaa taacgagact ataatagttt actgatttta tacgacatgt
     5581 ggaaccgatg gtcaacccga cgccccgggg cgggtatagg ttcggacggg cttttccggt
     5641 acaatccgcc gagaacgttc accccgggag cggccatgag acgggaaaag accacccttc
     5701 tgagaaaatt gtccatgtac ggcttcgacc tcgtgaccgg aagccacaac ttcttcgccc
     5761 tcctggagtt cgacgtgacg gacctacgcc gccatctccg ggcgctccgg gccgagggcc
     5821 ggggcgggtc cctcttcgcg tttcttctga aggccgtcgg caagtgcctg gaagagttcc
     5881 cggagttcaa cgccatgatc gaccgccgga agaccaccta tttcgacgag gtggacgtca
     5941 acatccccat cgagatcgag acggagggga tccggaaccc caagcagtac atcgtccggg
     6001 acatcaaccg caaatcgttg accgaggtgg atcgggagat cgcggcctcc aaggacgccg
     6061 gggacgacca cacgggttac gtcttttcca agggtctcca gcgtctcctg gggatcctcc
     6121 ccgccgggct cgtgatgttc ctgatgcgga gcatcctccg ccgtcacgat accgtgcaga
     6181 agctgtcggg cacggtcttc gtgacctccg tgagcatgtt ctccaacgtc cccgggtacg
     6241 tgatccccta cgcggggggc ccgaaggccg cctccttcgc gatcggaagc gtgtccaaga
     6301 aaccggtggt ccggggggac gaggtccggg tccgggagat gatcaacctc accgccatct
     6361 tcaaccacga cctgatcgac ggggccccgg ctgcccggtt catcaaccga ctgcgcaggt
     6421 acgtggaaac ctccttcgcg gagctgaccg ggtgagcgcg gcccccccct ccgcccggat
     6481 cctgctcctc accggggacc ggggagccgg gaagtccacc ctctgcgagc tcctggccgg
     6541 ggaagccgag gggcgcggca ttcccgcggg ggggatcgcc tgccccgcgg tcttcgggcc
     6601 tgatgggacg aagatcggct gccgggtccg cgacctggcc gccggggagg agcgggacct
     6661 gggatccacg gcgctggacc tgggcggtcc ccgctggaag ggctggagct tttcggagga
     6721 aggtttcgcg gcggcgaatc gggccgtcct cgcggcactg gaccggggcg cggggctcgt
     6781 gatcctggac gagatcggcc cggtggagct ccgcctggga gccgggctgg aaccttccct
     6841 acgggccctg gacgaaaggt atgccgggcc ggacctgccc gggccgggcg gccgccggga
     6901 cgtcgccgcc gggcccctcg ttctcctggt cgtccgcccg gaattggcgg aggaactggg
     6961 gcgccgcttc ccagggtccg cgatcatccg ggtcgatccc ggggaccgcc gggccgccct
     7021 ggacctcgcc cgggacgcgc tcttcagggc tccgagaccc tgaacgtcct tcacaatctt
     7081 cgtatcaccg gggtcaggcc cccggcaggg ccctcagggc ggagaagaag tgcagggcgc
     7141 tcccggccag gacgaagagg tgccagaccg cgtgaaacca cttcacccgc ttgatcacgt
     7201 agaacaccgc cccggccgtg tacgcgaccc cgcccgccgc caggagagcc acgctttcgg
     7261 cgggaaggtt ctccctcaga tggcccaggg cgggggcgat catccagccc atggccacgt
     7321 agcccagcac ggacaggagc ttccagcggt cgaaccacac ggccttgagg gcgatgcccg
     7381 ccagggccag ggcccactcg gcgccgaaga tccaccaacc cagggccgga ggcagggcgg
     7441 tcagggcgaa ggccgtgtag gtcccggcga tcaggacgta gatcgaggag tggtccagga
     7501 tttccaggat ccgcttgacc cggggattcg tgaccgagtg gtagagggcg ctcatcgtat
     7561 agaggacgac cagggaggag ccgaacacgg cgtaggacac gacgtgcctc gcgcttcccc
     7621 ggaggaccgc gaagaccacg aggatcacca gggccgccac ggaaagcagg gctcccgccc
     7681 cgtgggtcac cccgttggcg atctcctcgc ccgggctgta ccggcgctcg tgggaggaac
     7741 gatagggcag aagaatgtcg gacatggggc tcccttcgga cggaatcggg cgtcgccgat
     7801 cccgtaactc ggggatgccg tccggaagac cggcccgggg cgcgctcatc aggtttgacc
     7861 ggggattccc ccttcggttg cgcgcccccg ccgggtcagg tcactcgaag agcttggcca
     7921 gctcgggaaa gccctgctgg gccagatctt cccgggggat gaaccgcagg gaggccgagt
     7981 tcatgcagta ccgcaggccc gtgggggccg gcccgtcgtc gaagacgtgg cccaggtggg
     8041 cgcccgtgga cttggaccgg acctcgatgc gccgcatccc gtaggacagg tcctccacga
     8101 gttccaggct atcctgggag atcggggtcc agaaggaggg ccagccggtc ccggaatcgt
     8161 atttctcctt ggacgtgaac aggggagtcc cgtccacggc gtccacgtag atcccctggg
     8221 cgtggttgtt ccagtatgcg ttctggaagg ggggctccgt gccgccccgg gcgaccacgg
     8281 gaaccgcctg aagcaggtcg cgctccatgt ag
//
LOCUS       DAOTEI010000156         8312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6073_length_8312_cov_4.512535, whole genome shotgun sequence.
ACCESSION   DAOTEI010000156 DAOTEI010000000
VERSION     DAOTEI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6073_length_8312_cov_4.512535"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="P5313_13190"
     CDS             <1..892
                     /locus_tag="P5313_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81369.1"
                     /translation="ARPTVLANLIEQAAAPCLCALGTALVFRSGDFNLGGEGQAYAGG
                     ALSVLALLAAPSLPGPAGLAGGLAAGAFAGAFVALPSALSRRNLGADVLLSTFLVSQA
                     VVLVLDWAIAGPLRDPASNLIATPVVEAAYRLPRLAPPSWLSFAPVLAAAAAVALDRF
                     LRSSRRGLELTLFGRNPDFAVSMGFRVRAYAFWPLLASGALNGLAGALLALGANGRAV
                     RGMTGGIGWNGISIALLAGNDPRLILPAALFFSWLETGSRQASILAGISVDVGTVAKA
                     LVLLFATARFLGTARRKGTA"
     gene            889..1785
                     /locus_tag="P5313_13195"
     CDS             889..1785
                     /locus_tag="P5313_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81370.1"
                     /translation="MNPMETLAAEALAIALPILLAALGGLFTEAAGILNVALEGLIVA
                     GAFAFAVAAEGTGSLVLGVAAALACSAALALLAAAAADRLSANPFVAALAVNLLVAGA
                     VGLASEHVFGTKGVVPLDGLLSPRVELGFLSGIPFLGPVLFRQRGISYLLAAACAIAA
                     VAYSATPFGLRLRALGMKEGSLRIAGVDPRAVRYWAYAASGAACALAGIALARSIGAW
                     VPNIASGRGWIALVVVYLGRKRPAGLLAAALGFGLLFALSNAAQSVLSVPPELVLALP
                     YAATAAAALSGGARTRGRTDRG"
     gene            1790..2383
                     /locus_tag="P5313_13200"
     CDS             1790..2383
                     /locus_tag="P5313_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81371.1"
                     /translation="MGVGLVTRTARALGERIVPVRVLLARTTDFLGRTSLFSQYEGRL
                     RELNTRLSGSRGDDSTADEVRRELIEIRKGLRQAGYDLSLGNLDLALQGFRGDAAQGE
                     GFRRAVLFLGDRTLWCLAGEANHLELHDRLERLIHSTRSAPIRGKHYVWFRWDRDLLV
                     VSGAATETASDWEAFKVWCEGKERRLSLLSRMRRIPG"
     gene            2527..2952
                     /locus_tag="P5313_13205"
     CDS             2527..2952
                     /locus_tag="P5313_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY81372.1"
                     /translation="MFSVSARTQYGIRALAYLARTPGWEAIGAQIAEAEGISPKYLEG
                     ILTRLATAGLVRSERGKNGGYRLAKPPGGIVMAEVVAALDGEVRPVDCVDSLGICDHD
                     SSCRSRRFWIGLKFAIDEYLGNRTLADIIEEGADPVSTR"
     gene            2967..4130
                     /locus_tag="P5313_13210"
     CDS             2967..4130
                     /locus_tag="P5313_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRY81373.1"
                     /translation="MDRHFVYMDYNATTPLHPEVKARMIEDMEVYANASSLHEAGRGA
                     HARVEEARGAVARLIRAKDPSSVIFTSGGSESNNTVFATMFHLGRKGPRKGVVTTAIE
                     HPCVLNAAKWLAEEGFPVRFLGVDSKGRVDLEELRSSVGPDTLLVSIMAANNEIGTIE
                     DVASASRIAKSAGAFFHTDATQCAGKIPVDVEAWGADYLTLSCHKIYGPKGIGALYVR
                     KGAPLVPLIRGGHQEHGHRAGTYNNLGILGFGAAADLALRELPEYSARVRRLRERLRD
                     GLTSRLPYAHVNGHETEVLPNTLNLSFPGAEGESILLYLDLEGIQVSTGSACASGSLD
                     PSHVLLATGLGPELAHGSIRFSLGRDSTEADVDYVVQKLPPIIERLRRMSTVA"
     gene            4151..4759
                     /locus_tag="P5313_13215"
     CDS             4151..4759
                     /locus_tag="P5313_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRY81374.1"
                     /translation="MDAFTWLYSDIVKDHFMNPRNVFDPDEKFEADADGIVGNVKCGD
                     QMQFFLQVDEKDVIRDVRWKTYGCASAIASTSMLSEIVKGLPILEAYQLKPEDVSRRL
                     GGLPDNKIHCSVLGDKALRAAIDSWLAKTGREGAFKDVGSTVICTCLNITDRDIEQAV
                     KDGARTWEELQERTKIGTVCGGCKGRAEDLLVEYVHVFGHDE"
     gene            4794..5645
                     /locus_tag="P5313_13220"
     CDS             4794..5645
                     /locus_tag="P5313_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRY81375.1"
                     /translation="MILSEAGAKEFRAGVYRHFREEGRDFPWRRTRDPWLVLTSEVML
                     QQTRTEAVAARWDAWTSAYPSPASLAESPLAEVLGLWKGLGYNRRALALRNAAARIVH
                     EHGGAVPSGEAELRSLPGVGLYTARAVRAFAFGLPGVVIETNIRTVYLFYFYPDREKV
                     TDRELEPLVEATQDREDPRTWYYALMDYGAALKKREPNPGRRSAAYCRQSPFEGSHRQ
                     VRSAILHALSARGPLAAAALGETAAERVGPPAREPGFPERLARATEELAAEGFLEVRD
                     GTIRLRD"
     gene            5735..7330
                     /locus_tag="P5313_13225"
     CDS             5735..7330
                     /locus_tag="P5313_13225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY81376.1"
                     /translation="MESKRTILVVEDEALIALAERRALESFGYAVLTVPDGEKAVETA
                     LGNPDVDLVLMDIDLGRGMDGTEAARRILERRDIPVVFLSSHTEPDIVDRTERITSYG
                     YVVKDSGSTVLNASIRMAFRLFETRRREAEKEERLLKSERTYADIVRRIPSGFFVYRL
                     VAPGRLALEEANPEAERLTGIRLAEWRGRDFDEIWPRAREAGITDRYLEALRTGTAYE
                     VEDLQYSDDKIAGAFRVRAFALPDRRLAVSFEDITERKAAADALRRGRDLLDASQSVA
                     HVGSWLYEPSPGRLTWSDETYRLFGLEPASWTPTYEGFLEMVHPEDRGAVDAAYSRSL
                     ADGSPGYELEHRILRKGTGETRCVLEKCIHERDPEGRVLRSVGMVQDITDRKRLETEL
                     RKSEERYRSLFLLSPLPYVVTQDDRTVMVNPSAVRFFGADREEDLIGRDPREWLHPDS
                     RALSLERRRRILETGGVAEPAELKFILRDGREAYVLANLVRIEYRGAPALLSVFQDIT
                     AFKSSGQGGSGPGRTPAEQAPEE"
     gene            complement(7286..>8312)
                     /locus_tag="P5313_13230"
     CDS             complement(7286..>8312)
                     /locus_tag="P5313_13230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRY81377.1"
                     /translation="RVLYKEFASGRMSPDDLVTVLPEDCALPYNSSLMYLQPYMKLPV
                     RVLMQGLAVVSGNDAARALARAAAGSIEAFAGRMNEEAAALGLETTRFVEPSGLSEYN
                     LTTAREMALFAFRYLKDNPESLTELHSLRWIEFPVAEYMPPGVPPPKGRMVQYNNNKL
                     VRSYEGADGLKTGFIRESGFNLVATAERGGTRFLIVTLGGTGSSSRVGGDVRERDGRE
                     LLDWAFRNFVTVRPRVEHLPAVRLWKTADRSLTPVPASDFAFTVRSGDAPRVRARVEL
                     PEELVGPVPAGGRIGRVVFEAEGRELHTVDLVSPEEHPLGNVWIRFRDGIALFFRGLF
                     GRRPARA"
BASE COUNT         1241 a         2871 c         2903 g         1297 t
ORIGIN      
        1 cgcccgtccg acggtcctgg ccaacctgat cgagcaggcc gcggcgccct gcctctgcgc
       61 cctcgggacg gctctggtct tccgctccgg ggacttcaac ctgggcggcg agggacaggc
      121 ctacgccggg ggcgccctgt ccgtgctggc gctcttggcc gcgccgtccc ttcccgggcc
      181 cgcgggcctg gcgggagggc tggccgcggg cgccttcgcg ggggccttcg tggccctgcc
      241 gtccgccctg agccgccgga acctgggcgc ggacgtactg ctttccacct tcctggtgtc
      301 ccaggcggtg gtcctggtcc tggactgggc catcgcgggt ccgcttcgcg acccggccag
      361 caacctgatc gccacccccg tggtggaggc cgcgtaccgc ctcccgcggc ttgctccccc
      421 gtcctggctg agcttcgccc cggtcctggc cgcagccgcc gccgtcgccc tggatcgctt
      481 cctgaggtcg tcccgccggg gcctggaact caccctgttc gggcgcaatc cggacttcgc
      541 ggtctccatg ggattccggg tccgtgcata tgccttctgg ccccttttag cctcgggcgc
      601 cctgaacggc ctggcggggg ccctgctggc cctgggagcc aacggaaggg cggtccgggg
      661 tatgaccggg ggtatcggct ggaacgggat ctccatcgcc cttctggccg gaaacgatcc
      721 ccggttgatc cttcccgcgg ccctcttctt ttcctggctg gagacgggtt cccggcaggc
      781 ttccatcctt gcgggcatct ccgtggacgt gggcaccgtg gccaaggccc tggtcctcct
      841 gttcgctacc gcgaggttcc tgggtacggc ccggaggaag ggaaccgcat gaacccgatg
      901 gaaaccttgg ccgcggaagc cctggccatc gccctgccca tcctgctggc cgccctggga
      961 ggcctgttca ccgaggccgc gggcatcctg aacgtagccc tggagggcct gatcgtggcc
     1021 ggcgccttcg ccttcgcggt cgccgcggaa gggacgggaa gcctggtcct cggggtggcc
     1081 gccgccctgg cctgttccgc cgccctggcc cttctagccg cggcggccgc ggaccggctg
     1141 tccgcgaacc ccttcgtggc cgccctggcc gtcaacctcc tggtcgccgg agccgtgggg
     1201 ctggcctcgg agcacgtgtt cggcaccaag ggggtcgtcc ccctggacgg cctgctctcg
     1261 ccccgggtgg aactcgggtt cctgtccggg attcccttcc tggggcccgt cctcttccgg
     1321 cagcggggga tctcgtatct cctggccgcg gcctgcgcga tcgcggcggt cgcgtactcc
     1381 gcgacaccct tcgggctccg gctccgggcc ctgggcatga aggaaggatc cctccggatc
     1441 gcgggcgtgg atccccgggc ggtgcggtac tgggcctacg cggcgtccgg agccgcctgc
     1501 gccctggccg ggatcgccct ggcccgctcc atcggggcct gggtccccaa catcgcctcg
     1561 ggccgaggct ggatcgccct ggtggtcgtc tacctgggcc gtaaacggcc cgcgggcctc
     1621 ctggcggcgg ccctgggctt cgggctgctg ttcgccctgt ccaacgccgc ccaatccgtg
     1681 ctttccgtac cgccggaact ggtcctggcc ctgccgtacg cggcgaccgc cgcggcggcc
     1741 ttgtcgggag gggcgagaac ccgcgggcgg accgaccggg ggtgagggga tgggcgtcgg
     1801 cctcgtcacc cgcaccgccc gggccctggg cgagaggatc gtgccggtcc gggtcctgct
     1861 cgcccggacc acggacttcc tgggcaggac cagcctattc tcccagtacg agggcaggct
     1921 ccgggagttg aacacccggc tctcgggctc ccggggggac gactcgacgg cggacgaggt
     1981 gcgcagggag ctcatcgaga tccgcaaggg cctgcgccag gcgggctacg acctctccct
     2041 gggaaacctg gacctggccc tgcagggctt ccgcggcgac gcggcccagg gggaaggctt
     2101 ccgaagggcc gtccttttcc tgggggaccg gaccctctgg tgcctggccg gggaggcgaa
     2161 ccacctggag ctccacgacc gcctggagag actgatccat tccacccgct ccgcgccgat
     2221 ccggggtaag cactacgtct ggttccgctg ggaccgggac ctcctggtgg tctccggcgc
     2281 ggccaccgag acggcttcgg actgggaggc cttcaaggtc tggtgcgaag gcaaggagcg
     2341 ccgcctgtcc ctgctctccc ggatgcgcag gattcccgga tagggccgca tctgtcgatc
     2401 acggtccgcg ggttccctcc cggtgcatgt cgggtagttg acgagccatc gcaggctgag
     2461 gaccgcttga cggagagaga gttcctggat ataatcctat tatcccgata ggaaaaagag
     2521 gtttacttgt tctccgtctc cgcacgaacc cagtacggta tccgagcctt ggcctacctg
     2581 gcccgcacac ccgggtggga ggccatcggg gcccagatcg cggaggcgga gggtatatcc
     2641 cccaagtacc tggagggcat cctgacccgg ctggccacgg cgggcctggt ccggagcgag
     2701 cgcggcaaga acgggggata ccgcctggcc aaaccgccgg gggggatcgt catggcagag
     2761 gtcgtggccg ccctggacgg ggaggtccga cccgtggact gcgtggactc cctggggatc
     2821 tgcgaccacg actcctcctg ccgatcccgg aggttctgga tcggcctaaa attcgccatc
     2881 gacgaatacc tgggcaaccg gaccttggcg gacatcatcg aggaaggcgc ggacccggtg
     2941 tccacgcggt aggagagagg aaccgaatgg acaggcattt cgtatacatg gactacaacg
     3001 ccacgacccc cctgcatccg gaggtcaagg cccggatgat cgaggacatg gaggtgtacg
     3061 cgaacgcctc cagcctccac gaggcgggca ggggcgccca tgcccgagtc gaggaggccc
     3121 ggggcgcggt cgcccgcctg atccgcgcca aggacccctc atccgtcatc ttcaccagcg
     3181 gagggtcgga gtcgaacaac accgtcttcg cgaccatgtt ccacctgggc cgcaagggcc
     3241 cccggaaggg cgtcgtaacc accgccatcg agcacccctg cgtcctcaac gcggccaagt
     3301 ggctggcgga ggaggggttc ccggtccgtt tcctgggagt ggactccaag ggccgcgtgg
     3361 acctggagga gcttcggtcc tccgtcggac ccgacaccct gctcgtgtcg atcatggcgg
     3421 ccaacaacga gatcggcacg atcgaggacg tggcctccgc ctcgcgcatc gccaagtccg
     3481 cgggcgcctt cttccacacc gacgccaccc agtgcgcggg aaagatcccc gtggacgtcg
     3541 aggcctgggg ggcggattac ctgaccctgt cctgtcacaa gatctacggc cccaagggga
     3601 tcggcgccct ctacgtccgc aagggagccc ccctggttcc cctcatccgg ggaggccacc
     3661 aggagcacgg tcaccgggcg ggaacctaca acaacctcgg catcctgggc ttcggggctg
     3721 ccgcggacct tgccctccgg gagctcccgg agtactccgc ccgggtccga cgcctgcgcg
     3781 agcgcctgcg ggacgggctc acctcccggc tgccctacgc ccacgtcaac ggccacgaga
     3841 ccgaggtcct gcccaacacc ctgaacctct ccttccccgg ggcggagggg gagagcatcc
     3901 tgctgtacct ggacctggag ggcatccagg tctccacggg ctccgcctgc gcctccggga
     3961 gcctggatcc cagccacgtc cttttggcca cgggcctggg gccggagttg gcccacgggt
     4021 ccatccggtt cagcctgggc cgggactcca cggaggcgga cgtggactac gtcgtccaga
     4081 agctgccccc catcatcgag cgcctccggc gcatgtccac cgtagcctga gcgacggaca
     4141 aggagagaac atggacgcct tcacctggct gtattccgat atcgtcaagg accacttcat
     4201 gaacccccgg aacgtcttcg acccggacga gaagttcgag gccgacgcgg acggcatcgt
     4261 ggggaacgtg aagtgcgggg accagatgca gttcttcctc caggtggacg agaaggacgt
     4321 gatccgggac gtccggtgga agacctacgg ctgcgccagc gcgatcgcgt cgacctcgat
     4381 gctcagcgag atcgtgaagg gcctgcccat cctggaggcc taccagctca agcccgagga
     4441 cgtgtccagg cgcctcgggg gcctgccgga caacaagatc cactgctccg tcctgggcga
     4501 caaggccctg cgggcggcca tcgactcctg gctggccaag acgggtcggg agggagcctt
     4561 caaggacgtg ggctccacgg tgatatgcac ctgcctcaac atcacggacc gggacatcga
     4621 gcaggccgtg aaggacgggg cccggacctg ggaggagctc caggagcgca ccaagatcgg
     4681 cacggtctgc gggggctgca agggccgggc ggaggacctc ctggtcgagt acgtccatgt
     4741 cttcggacac gacgagtagg attcccgggc cgccgattcc ggtcagcctc cccttgatcc
     4801 tttcggaggc cggggcgaaa gaattccgcg ccggggtcta ccggcacttc cgggaggagg
     4861 gccgggactt cccctggcgc cggacccggg atccatggct cgtcctgacc tcggaggtca
     4921 tgctccagca gacccgcacc gaggccgtgg cggccaggtg ggacgcctgg acctccgcct
     4981 atccgtcccc ggcctccctg gccgagtcgc ccctggccga ggtgctggga ctctggaagg
     5041 gcctcgggta caatcgccgc gccctggccc tgcggaatgc ggcggcccgg atcgtccacg
     5101 agcacggagg agcggtcccg agcggcgaag cggaactgag gtccctgccc ggggtgggtc
     5161 tctacacggc ccgggcggtt agggcattcg ccttcggact tccgggtgtg gtgatcgaga
     5221 cgaacatccg gacggtctac ctgttctact tctacccgga ccgggagaag gtcacggatc
     5281 gggagctcga acccctggtc gaggccaccc aggaccggga ggatccgcgg acctggtact
     5341 acgccctgat ggactacggg gcggccctga agaagcggga gcccaatccc ggccgacgat
     5401 ctgccgccta ctgccggcag tcgcccttcg aggggtccca ccgacaggtg cggtccgcga
     5461 tcctccatgc cctctccgcc cggggcccct tggctgcggc cgcactgggc gagaccgcgg
     5521 cggagcgggt cgggcccccc gcccgggaac ccgggttccc ggagaggctc gcccgggcta
     5581 cggaggaact ggccgcggag ggcttcctgg aggtccggga cgggacgatc cggctgaggg
     5641 actgagccgg ccttgcgcgg gaggcggaaa tgtgtagagt ttcatggtat atcgcgggtc
     5701 ccgaagtcct cacctgccga ggccggagtc gaatgtggaa agcaagcgga cgatcctggt
     5761 agtcgaggac gaggccctca tcgccctggc cgagcggcgg gccctggagt ccttcggata
     5821 cgccgtcttg accgtcccgg acggggagaa ggccgtggaa acggccctgg ggaatccgga
     5881 cgtggacctg gtgctcatgg acatcgacct gggcaggggc atggacggca ccgaggccgc
     5941 ccgccggatc ctggagcgcc gggacatacc cgtcgtcttc ctgtcgagcc acacggagcc
     6001 ggacatcgtc gaccggaccg agcggatcac ctcctacggg tacgtggtga aggattcggg
     6061 ctcgaccgtc ctgaacgcct ccatccgaat ggcgttccgt ctcttcgaaa cccgccgccg
     6121 ggaggccgag aaggaggaga ggcttctcaa gtcggaacgc acctacgccg acatcgtccg
     6181 gcgcatcccc tccgggttct tcgtctaccg cctggtcgcc ccgggccgcc tggccctgga
     6241 ggaggccaat cccgaggccg agcgtctgac gggcatccgc ctggcggagt ggagggggcg
     6301 ggatttcgac gagatttggc cccgcgcccg ggaggcggga atcaccgacc gctacctgga
     6361 agccctgcgg accgggaccg cctacgaggt cgaggacctg cagtactcgg acgacaagat
     6421 agcgggagcc ttccgggtgc gcgccttcgc gctcccggac cggcggctcg cggtctcgtt
     6481 cgaggatatc acggagcgga aggccgcagc ggacgccctc cgccggggcc gggacctcct
     6541 ggacgcctcc caatccgtgg cgcacgtggg aagctggctc tacgagccgt ccccgggaag
     6601 gctcacctgg tccgacgaga cctaccggct cttcggcctg gaacccgcgt cctggacgcc
     6661 gacctacgag ggttttctcg agatggtcca tccggaagac cggggagccg tggacgctgc
     6721 ctattcccgg tccctggcgg acggaagccc gggctacgag ctggagcaca ggatcctgcg
     6781 gaagggcacg ggggagacgc gctgcgtcct ggagaaatgc atccacgagc gggacccgga
     6841 gggccgcgtt ctccggtccg tgggcatggt ccaggacatc accgaccgga aacggctgga
     6901 gaccgagctt cggaagagcg aggagcggta ccggtccctc ttcctgctct cacctctgcc
     6961 ttacgtggtg acccaggacg acaggaccgt gatggtcaat ccgtccgcgg tgcgcttctt
     7021 cggggcggac cgggaggagg acctgatcgg ccgggatccc cgggaatggc tgcacccgga
     7081 ctcccgcgcg ctgtccctgg agcggcgccg caggatcctg gagacgggag gggtcgcgga
     7141 gcccgcggag ctgaaattca tcctccggga cggaagggaa gcctacgtcc tggccaatct
     7201 cgtgcggatc gagtataggg gggctccggc cctgctgtcg gtcttccagg acatcactgc
     7261 cttcaagtcg tcggggcagg gcggatcagg cccgggccgg acgccggccg aacaagcccc
     7321 ggaagaatag ggcgatcccg tcccggaacc gtatccagac attgcccagg ggatgttcct
     7381 ccggggacac gagatccacg gtatggagtt cccggccctc cgcctcgaag acgacgcggc
     7441 cgatccgccc gcccgccggg accggtccga ccagctcctc cggcagttcc acccgggccc
     7501 ggacccgggg cgcgtccccg gaccgcaccg tgaaggcgaa gtcggatgcc ggcacgggcg
     7561 tgagggacct gtccgcggtt ttccagaggc gcacggccgg caggtgttcg actcgcggcc
     7621 ggaccgtgac gaagttccgg aatgcccagt ccaggagctc ccggccgtcc cgttcccgca
     7681 cgtccccgcc tacccgggag gaggagcccg tcccgcccag ggtcacgatc aggaaccggg
     7741 tgccgccgcg ctcggcggtg gccaccaggt tgaagccgct ttcccggatg aatcccgtct
     7801 tgaggccgtc cgcgccctcg tagctgcgga ccaatttatt gttgttgtac tggaccatgc
     7861 ggcccttggg aggaggaacg ccgggcggca tgtactccgc gacggggaac tcgatccacc
     7921 gcagggagtg gagctccgtc agggattcgg ggttgtcctt gaggtagcgg aaggcgaaga
     7981 gggccatctc ccgggcggtt gtgaggttgt actccgatag ccccgagggt tccacgaagc
     8041 gggtagtctc caggcccagc gccgcggctt cctcgttcat cctgcccgcg aaggcctcga
     8101 tgctcccggc cgcggcccgg gccagggcgc gggccgcgtc gtttccggac accaccgcca
     8161 gcccctgcat gagcactcgt accggcagct tcatgtaggg ctgtaggtac atgaggctgg
     8221 agttgtaggg aagggcgcag tcctccggca ggaccgtcac gaggtcgtcc ggggacatgc
     8281 gtccggaggc gaattccttg tacaggaccc gc
//
LOCUS       DAOTEI010000157         8280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6097_length_8280_cov_5.335319, whole genome shotgun sequence.
ACCESSION   DAOTEI010000157 DAOTEI010000000
VERSION     DAOTEI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6097_length_8280_cov_5.335319"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="P5313_13235"
     CDS             complement(<1..537)
                     /locus_tag="P5313_13235"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRY81378.1"
                     /translation="MTGVILQARLGSTRLPRKALLPFGDGTLLESCLRRLSRVPADLR
                     VLATEPGSAPELGPRAEALGWKVFVGSEEDVLARYCGAIREYRISRVIRATGDNPFVS
                     PEAAESLLSDPRTAASDYAAYQGLPLGFGVEVVRASALVEAEAESSDPYEREHVCPFL
                     YRRPGRFSIYRPEAPGSLR"
     gene            complement(534..1499)
                     /locus_tag="P5313_13240"
     CDS             complement(534..1499)
                     /locus_tag="P5313_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81379.1"
                     /translation="MSTIPTTLDDRPVPYTVVGGQKSRACPEGLPVSVVLLNRGSRLY
                     RARLLADLERIGFDSILSIEAGSETPDVESLSGRFPQVRFLFLKEEVTTGEKVNLGIR
                     ESCGPFVFVLWNDQKLSTAALSSRFFERLSEQDFLCTAPFLSSGGGEPVPTLLAPAFE
                     GAGLRILSLTPQKDGDKTLFPFDGCGIYSRERFVRLGGYDGTLPDPWWQKMDFGFRAW
                     LWGEEIRMAQALRMGYEGVPPTEDTTPGPSYGRFHLKNLAVRVREDGGMLPLGAFPAY
                     LFRASRDPFRAAGEFRAAREWVRINRYRFRWDARSVVDLWEPLAP"
     gene            complement(1474..2997)
                     /locus_tag="P5313_13245"
     CDS             complement(1474..2997)
                     /locus_tag="P5313_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HRY81380.1"
                     /translation="MTAIAVLDCVGIRDLALSPLGEGPSALERVLEKVRGLPGVGQVV
                     LVSDRDLPGAPVPVVKVPGPGSRPFLEALEAVLAAVPAGAEGRDALIRIRGDAPFLDP
                     ALTGRMLEDFRRYRAEYYFADGFPAGVAPEILAPRILPALRALAEKVPEPLGEEGLFP
                     VIQKDINSFDLETEISRVDLREYRLSLTCDTRRNRMVCERLLDLGAGDAESILDLVPA
                     NLGLLRTLPAFVSVQVAGGCPQACSYCPYPGFGGDILSRRDFLPVERYSRLMDETADL
                     CGDAVVDLSLWGEPSLHPDFPALVKSTLRHPGLSLIVETSGVGWDDDTVEKAAALADG
                     RLDWIVSLDETDPEAYARLRGPGFEEAKARTGTLMRLFPGRVHVQAVRMKENEERLEA
                     FYRGWKARTENVIIQKYDSFCGTLPDRSVADLSPLDRRPCWHLARDVCVLLDGSVPVC
                     RECRAPGLPEGAEILGNVFEEGLAALWARSEPWFRRHTAKDYPAICGRCDEYHTYNA"
     gene            3301..6804
                     /locus_tag="P5313_13250"
     CDS             3301..6804
                     /locus_tag="P5313_13250"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRY81381.1"
                     /translation="MNIERRFTRPGRGPYEGLSWERRVSEIRNLDGRTVFRMEGVIVP
                     SGWSQIATDILAQKYFRKAGIPEGKARSWESFVPEDQRTLAGPGPKAGAEHDARQVFH
                     RLAYTWLRWGTEEGWFDTEEDGKAFYDETCAMLARQMAAPNSPQWFNTGLHAVYGIEG
                     PAQGHFYMDPATGEVVKSSSAYARPQPHACFILDIQDDLVNEGGIMDLVTREARLFKY
                     GSGTGSNFSKLRGALEPLSGGGVSSGLLSFLKIADRSAGAIKSGGTTRRAAKMVILDA
                     DHPDIESYVDWKPREEYKVACLAAGSALIRYHLEKVRSAVARTDLPPASRTSLDANPA
                     LRDAARTALRAGIPAAFLHQVLLRLSQGDAAPDLPQYDTSWEGEAYNTVSGQSSNNSV
                     RVSTKFMEAVVKNRDWDLTARTDGKTMRTVKARALWDRFAQAAWSCADPGLQFHGTVN
                     EWHTCPADGEIRGSNPCSEYMFLDDTACNLASLNLLSFYDEAERRFDADGYRHAVRIW
                     TTILEISVAMAQFPSKEIARKSYDYRTLGLGFANLGTLLMVMGLAYDSDEGRAVAAGL
                     SAALTGEAYAQSARMAAQRGPFARYEANKDAMLRVVRNHRRAAHGEPTSAYEGLTVTP
                     KPLDPALCPEPIRTAARASWDEALALGRAHGFRNAQVSAIAPTGTIGLLMDCDTTGVE
                     PDFALAKFKKLAGGGYFRIINRSVPPALRALGYDEEAVREIVAYCVGSGTLAEAPGVS
                     LARLAEKGIPKEALDKAEAALKDAIGIEGVFTPHILGKETFDALGVPEATWSLPGFSL
                     LRHLGFTPEEVAAAEDFACGTLGLEGAPRLKKAHYPVFDTATPSGKRGTRSIAWQAHV
                     AMMAAVQPFVSGAISKTINMPASASVEDVKGAHMLAWKSMLKSIALYRDGSKLSQPLS
                     ALAPGSDPVADALVALQYEEPAPALSAAVVPESRASSPPAAPARTRTSLPNRRSGYTQ
                     KAKIGGHSVFVRTGEYDDGSLGEIFLDMHKEGAAFRSLLNSFAISVSLGLQYGVPLDE
                     FVDAFTFTRFEPNGMVQGHDLIKMSTSVLDYVFRDLAITYLNRYDLGQVKPEDLISTA
                     TQNGHARNGHVNLAPSGDPAKAPSSAQPSPTAPKPDARPRTARLKGYEGDPCPVCGHL
                     TLVRNGTCLKCETCGSTTGCS"
     gene            complement(6912..7082)
                     /locus_tag="P5313_13255"
     CDS             complement(6912..7082)
                     /locus_tag="P5313_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY81382.1"
                     /translation="MAYKVTDACVNCGACDPECPVEAISEKDGARWIDPDKCTDCGSC
                     ASVCPTEAIIAG"
     gene            7338..>8280
                     /locus_tag="P5313_13260"
     CDS             7338..>8280
                     /locus_tag="P5313_13260"
                     /inference="COORDINATES: protein motif:HMM:NF014281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY81383.1"
                     /translation="MKNLKLGLKLIGSFSIVAGITLALGILGFYAITQLSGYLNNIGR
                     NRIPDLQSLAALNRERMSIRAQTLEMYSFETQADTEAGIRKILENRQKSWKVVDEAWT
                     AFLAIPRQSERGRALLASAKEEYKAWRDIYVELDQLVGRIGAVSDPGEKAVLYAEYRR
                     LVARMVPISDKLGATCDELTANNLTNTNRMIDTYTILAETLTRVSLSAMILAVLLALG
                     LGIILSRGITVPMARGVEFARRLSEGDLTADLAVVQKDEIGKLADALREMRDRLKEIM
                     SGVKTAADNVYGGSQQISATAQQLSQGATEQAANAEEV"
BASE COUNT         1457 a         2841 c         2732 g         1250 t
ORIGIN      
        1 gcggagggat cccggcgcct cgggccggta gatcgaaaaa cgcccgggcc ggcggtagag
       61 gaagggacag acatgctccc gctcgtaggg gtccgaggac tccgcctccg cttccaccag
      121 ggcggaagcc cgtaccacct cgaccccgaa gcccaggggc aggccttgat aggccgcgta
      181 gtcggaggcg gcggtacggg gatccgaaag gagggactcg gcggcctcgg ggctgacgaa
      241 ggggttgtcc ccggtggcac ggatcaccct ggaaatccgg tactcccgga tcgccccgca
      301 gtaccgggcc aggacgtcct cctcggaccc cacgaagacc ttccatccca gggcttccgc
      361 ccggggcccc agctccgggg cgctcccggg ctccgtggcc aggacgcgga ggtccgcggg
      421 cacccgggag aggcggcgca ggcaagattc caggagggtt ccgtccccga agggaaggag
      481 ggccttgcgg ggaagccggg tggaacccag gcgggcctgg aggatgacgc cggtcatggg
      541 gccaggggtt cccagagatc gaccacgctc cgggcatccc accggaagcg gtaccggttg
      601 atccgaaccc attcccgggc cgcccggaac tcccccgcgg cccggaaggg gtcccgggag
      661 gcacggaaca ggtaggccgg gaaggccccc agggggagca tgccgccgtc ctcccggacc
      721 cggacagcga ggttcttgag gtggaagcgg ccgtaggacg gcccgggggt cgtgtcctcc
      781 gtaggtggaa cgccctcgta gcccatccgc agggcctggg ccatgcggat ctcctccccc
      841 cagagccagg cgcggaatcc gaaatccatc ttctgccacc agggatccgg cagggttccg
      901 tcgtatcctc ccaggcggac gaacctctcc cgggaataga tcccgcagcc gtcgaagggg
      961 aagagggtct tgtccccgtc cttctgggga gtcaaggaaa ggatccgcag gccggcgccc
     1021 tcgaaggccg gggccaggag ggtcgggacc ggctcgcccc ccccggagga gaggaaggga
     1081 gcggtgcaca ggaagtcctg ctccgagagc cgttcgaaga aacggctgga cagggccgcg
     1141 gtggagagct tctggtcgtt ccagaggacg aagacgaagg gaccgcagga ctcccggatc
     1201 cccaggttca ctttctcccc ggtcgtgacc tcctccttca ggaagagaaa ccgcacctgg
     1261 gggaaacggc cggacaggga ttccacgtcg ggggtctcgg agccggcctc tatggagagg
     1321 atggaatcga agccgatccg ttccaggtcc gccaggagcc gggcccggta cagccggctg
     1381 ccccggttca ggaggaccac cgatacggga aggccttccg gacaggcgcg ggacttctgc
     1441 ccgcccacca ccgtgtaggg gacggggcgg tcgtcaagcg ttgtaggtat ggtactcatc
     1501 gcagcgtccg cagatcgcgg ggtagtcctt cgccgtgtgg cgccggaacc agggctcgct
     1561 ccgggcccag agggcggcaa ggccctcctc gaacacgttg cccaggatct ccgcgccctc
     1621 cggaagcccg ggggcccggc attcccggca gaccggtacg gagccgtcca ggaggacgca
     1681 gacgtcccgg gccaggtgcc agcagggcct tcgatccagg ggggagaggt ccgcgacgga
     1741 ccggtccggc agggtgccgc agaaggaatc gtacttctgg atgatcacgt tctccgtccg
     1801 ggccttccag ccccggtaga aggcctccag gcgctcctcg ttctccttca tccgtaccgc
     1861 ctggacgtgg acccgtcccg ggaagaggcg catcagggtc ccggttcggg ccttcgcctc
     1921 ctcgaacccc ggcccccgga ggcgggcgta ggcttcgggg tcggtctcat ccagggaaac
     1981 gatccaatcc aggcgtccgt ccgccagggc cgcggccttc tcgaccgtgt cgtcgtccca
     2041 ccccaccccg gaggtctcca cgatgaggga tagaccgggg tgccggaggg tggacttgac
     2101 cagggccggg aaatcgggat ggagcgaggg ctccccccag agggaaaggt ccaccacggc
     2161 gtccccgcag aggtccgcgg tctcgtccat cagccgggag tagcgctcga cgggcaggaa
     2221 gtcccgccgg gacaggatat cccctccgaa cccgggatag gggcagtagg agcaggcctg
     2281 gggacagccc ccggccacct ggacggagac gaaggccggg agggtgcgga ggagcccgag
     2341 gttcgcaggc accaggtcca ggatggactc cgcgtccccg gcgcccaggt ccaggaggcg
     2401 ctcgcagacc atccggttgc gccgggtgtc gcaggtcagg gacaggcggt actcccggag
     2461 gtcgacccgg gagatctccg tctccaggtc gaaggagttg atgtccttct ggatgaccgg
     2521 aaagagtccc tcctcgccca ggggttccgg gaccttttcc gccagggccc ggagggccgg
     2581 gaggatccgg ggggccagga tctccggggc cacccccgcg gggaagccgt cggcgaagta
     2641 gtactccgcc cgatacctgc ggaagtcctc cagcatccgg ccggtcaggg ccgggtcgag
     2701 gaagggggcg tccccccgta tccggatcag ggcgtcccgg ccttccgcgc ccgcggggac
     2761 ggccgccagg accgcctcga gagcctccag gaagggccgg gaaccgggtc ccgggacttt
     2821 gacgacgggc accggggcac cgggcaggtc gcggtccgag accaggacga cctgccccac
     2881 ccccggaagg ccccggacct tttccaggac ccgttccagg gcggatggtc cctcccccag
     2941 ggggctcagg gcgagatccc ggattccgac gcaatcgagc acggctatgg ctgtcatacc
     3001 ctatccagta tcggccgcac ggccgggggt cgttagggac gggtccgcag gacgtcccgg
     3061 atcgagtgta cacccccggg cggaggcgat caatctatgc cggaaggatg cttgccgcgg
     3121 gccgccggcc ccttcgggct catggcgaca ataggcaggg tccggatctc caattcatag
     3181 ggtttcctct attataccac ctattgaagt ttagccttgc atggccacaa tatgttgtgg
     3241 tatagtcgcc gtctgcgccg aaaatgtact agagcgcgga taccataaag gagcgtcgcc
     3301 atgaacatag agcgccgatt cacccgcccc ggccgcggcc cctacgaggg gctctcctgg
     3361 gaacgcaggg tttccgagat acgcaacctg gacggtcgga cggtattccg catggagggg
     3421 gtgatcgtgc cctccggatg gagccagatc gccacggaca tcctggccca gaagtacttc
     3481 cgcaaggcag ggatccccga gggcaaggcc cggagttggg agtccttcgt tcccgaggac
     3541 cagcggaccc tggccggccc gggccccaaa gccggggcgg agcacgacgc ccgccaggtc
     3601 ttccaccgcc tggcctatac ctggctccgc tgggggaccg aggagggctg gttcgacacc
     3661 gaggaagacg gcaaggcctt ctacgacgag acctgcgcca tgctggcccg tcagatggcg
     3721 gcccccaact ccccccagtg gttcaacacg gggctccacg cggtgtacgg gatagagggt
     3781 cccgcacagg gccacttcta catggatccc gccaccgggg aggtggtgaa gtcctccagc
     3841 gcctacgccc ggccccagcc ccacgcctgc ttcatcctgg acatccagga cgacctggtc
     3901 aacgagggcg ggatcatgga cctggtcacc cgggaagccc ggctcttcaa gtacgggtcc
     3961 ggcacggggt ccaacttctc caagctccgg ggggccctgg aacccctgtc cgggggcggg
     4021 gtctcctcgg ggctcctgtc gttcctcaag atcgcggacc gctccgcggg agccatcaag
     4081 agcgggggga ccacccgccg ggccgccaag atggtcatcc tggacgcgga ccacccggac
     4141 atcgagtcct acgtggactg gaagccccgg gaggagtaca aggtggcctg cctggccgcg
     4201 gggagcgccc tgatccggta ccacctggag aaggtccgct ccgcggtcgc ccggacggac
     4261 ctgccgcctg cctcccggac ctccctggac gcgaacccgg ccctccggga cgctgcgcgg
     4321 accgccctcc gggccgggat cccagcggcc ttcctccacc aggtcctcct gcggctctcc
     4381 cagggggacg ccgcccccga cctcccccag tacgacacct cctgggaggg ggaggcctac
     4441 aacaccgtca gcggccagtc ctccaacaac tccgtccggg tctccaccaa gttcatggag
     4501 gcggtggtca agaacaggga ctgggacctg accgcccgga ccgacgggaa gaccatgcgg
     4561 accgtcaagg cccgggccct ctgggaccgc ttcgcccagg ccgcctggtc ctgcgcggac
     4621 ccggggctcc agttccacgg caccgtgaac gagtggcaca cctgcccggc ggacggggag
     4681 atccggggct cgaacccctg ctcggagtac atgttcctgg acgacacggc ctgcaacctg
     4741 gcctccctca acctcctttc cttctacgac gaggccgagc gcaggttcga cgcggacggg
     4801 taccggcacg ccgtgcggat ctggactacg atcctggaga tctccgtggc catggcccag
     4861 ttcccctcca aggagatcgc ccggaagagc tacgactacc ggaccctggg gcttggcttc
     4921 gccaacctgg ggaccctgct catggtcatg ggcctggcct acgattccga cgagggccgg
     4981 gccgtggccg cagggctctc cgccgcgctc accggggaag cctacgccca gagcgcccgg
     5041 atggccgccc agcgggggcc cttcgcccgg tacgaggcca acaaggacgc catgctccgg
     5101 gtcgtccgca accaccgccg cgcggcccac ggcgagccga cctcggcgta cgagggcctg
     5161 acggtgacgc ccaaacccct ggatcccgcc ctctgcccgg aacccatccg gaccgcggcc
     5221 agggcctcct gggacgaggc cctggccctg ggcagggccc acggcttccg gaacgcccag
     5281 gtaagcgcca tagcccccac gggcaccatc ggccttctca tggactgcga taccaccggg
     5341 gtagagcccg acttcgccct ggccaagttc aagaagctgg cgggaggcgg ctacttccgg
     5401 atcatcaacc gcagcgtgcc ccccgccctc cgggccctgg gctacgacga ggaggcggtc
     5461 cgggagatcg tcgcctactg cgtgggaagc ggcaccctgg cggaggcccc gggcgtaagc
     5521 ctggcccgtt tggccgagaa ggggattccc aaggaggccc tggacaaggc ggaggcggcc
     5581 ctgaaggacg cgatcgggat cgagggggtc ttcacccccc acatcctggg caaggagacc
     5641 ttcgatgccc tgggagtccc cgaggccacc tggtccctcc cgggattctc cctgttgcgg
     5701 cacctgggct tcaccccgga agaggtcgcc gccgcggagg acttcgcctg cgggaccctg
     5761 ggcctggagg gggccccccg gctcaagaag gcccactacc ccgtcttcga caccgccacc
     5821 ccctcgggca agcggggaac ccggtccatc gcctggcagg cccacgtggc catgatggcg
     5881 gcggtccagc ccttcgtgtc cggggccatc tccaagacca tcaacatgcc cgcttccgcc
     5941 tccgtcgagg acgtgaaggg agcccacatg ctggcctgga agagcatgct caagagcatc
     6001 gccctgtacc gggacgggtc caagctctcc cagcccctgt ccgccctggc cccgggctcg
     6061 gaccccgtgg cggacgccct ggtggctctc cagtacgagg agccggcccc ggccctttcc
     6121 gcggccgtcg tccccgaatc ccgcgcctcg tccccgcccg ccgcgcccgc ccggacccgg
     6181 acctccctgc ccaaccgccg ctcgggctac acccagaagg ccaagatcgg cggccactct
     6241 gtcttcgtgc gcaccggcga gtacgacgac gggtccctgg gggagatctt cctcgacatg
     6301 cacaaggagg gcgcggcctt ccggtccctc ttgaactcct tcgccatctc cgtgtccctg
     6361 ggcctgcagt acggggtgcc cctggatgag ttcgtggacg ccttcacctt cacccgtttc
     6421 gagcccaacg ggatggtcca gggccacgac ctgatcaaga tgtccaccag cgtgctggac
     6481 tacgtcttcc gggacctggc catcacctac ctgaaccgct acgacctggg ccaggtcaag
     6541 cccgaggacc tgatctccac ggccacccag aacggccacg cccggaacgg ccacgtgaac
     6601 ctggcgccct cgggagaccc tgccaaggcg ccctcctcgg cccagccctc cccgacggcg
     6661 cccaagcccg acgcccggcc ccggacagcc cggctcaagg gctacgaggg cgatccctgc
     6721 cccgtctgcg gccacctgac cctggtccga aacggtacgt gcctgaagtg cgaaacttgc
     6781 ggctctacca cgggatgtag ttagcaagaa aacgcaggtt tcgcatttca ccggggcaga
     6841 cggaagcggc gtcagccgct tccgaataaa aaaaccgccc cctcgggggc ggtttttttg
     6901 cgcgacgggg cttacccggc gatgatcgcc tcggtggggc acacggaagc gcagctcccg
     6961 cagtccgtgc acttgtccgg atcgatccag cgggctccgt ccttttcgga aatggcttcg
     7021 accgggcact ccgggtcgca ggctccgcag ttcacacagg catcggtcac cttataggcc
     7081 atgaaaacac ctctcgtatg tggatttcga cctcatagta ccacgatccg gggaagggcg
     7141 caagggcagc gagactgtcc caagctcgcg tgtatttata gtttttatcg ttttatacat
     7201 ttcgataata gtaagttata tttttttatg ctgtaaatat ttacaatttt tttccgcttg
     7261 gcggaaatcc ttatgcatga tataatatct atattatctc tattttagaa tcattctgat
     7321 tatcattagg aggaaccatg aaaaacctca agctcggcct gaagctgatc ggctcctttt
     7381 cgatcgtcgc cgggatcacc ttggccctgg gaatcctcgg gttctatgca atcacacaat
     7441 tatccgggta cctgaacaat atcggaagaa accgaattcc ggaccttcag tcgttagccg
     7501 ccctcaaccg agagaggatg tccatccgcg ctcaaacctt ggaaatgtac tcctttgaaa
     7561 cccaggccga cacggaggcg ggaatccgga agatcctgga aaatcgacag aagagctgga
     7621 aagtcgtgga tgaggcatgg acagccttcc tcgcgatccc ccggcaatcc gaacggggca
     7681 gggccctgct cgcaagcgcc aaggaggagt acaaggcctg gcgcgacatc tatgtcgaac
     7741 tcgaccaatt ggtcggccgg atcggcgccg tctccgatcc cggcgagaaa gcggtcttgt
     7801 atgcggagta ccgtcggctc gtcgcgcgga tggtaccgat ttccgacaag ctgggagcga
     7861 cctgcgatga actgaccgcg aataacctga cgaacacgaa ccggatgatc gatacctaca
     7921 cgatcctcgc cgagacccta actcgcgtga gcctctcggc catgatactg gccgtgctct
     7981 tggccctcgg gctcgggatc atcctgagcc ggggcatcac cgtgcccatg gcccgaggcg
     8041 tcgagttcgc ccgcaggctc tcggaagggg atctgaccgc ggatctggcg gtggtccaga
     8101 aggacgagat cggcaagctg gccgacgccc tccgggaaat gcgggaccgg ttgaaggaga
     8161 tcatgagcgg agtcaagacc gcggcggaca acgtctacgg cggctcgcag cagatctcgg
     8221 ccactgccca gcagctgtcc cagggggcca cggagcaggc ggccaacgcc gaggaggtct
//
LOCUS       DAOTEI010000158         8180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6194_length_8180_cov_4.471385, whole genome shotgun sequence.
ACCESSION   DAOTEI010000158 DAOTEI010000000
VERSION     DAOTEI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6194_length_8180_cov_4.471385"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="P5313_13265"
     CDS             <1..1091
                     /locus_tag="P5313_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY81384.1"
                     /translation="WMASVPRIWESVKDGIYRNIRQTGGVKAALFHFFVAVGSAFSHF
                     RNQLLGLLPDFGNRVPILDRILPIIPLVLLAPLNALGGILVFKKIKHKLGGNFIAGIS
                     GGGALPSAVDQFFGALGVLILEGYGLTETAPVLGVRPQHKPVLGTVGPALKGTEIKIV
                     GENGVVLPAGQKGLILVKGPQVMKGYYKRPDLTEKVLSADGWLDTGDLGMLTRSGEIK
                     ITGRAKDTIVLLGGENIEPLPIEQKLCESEYIKQTIVLGQDKKFLAALIVPDADAINA
                     WAKDNHVYIVDYETLLQQPEVQELLDYEVKELISPKNGFKNFERIFRFKLLPVPFEVG
                     RELSAKQEIKRHVINEIYAKDIAALFKD"
     gene            complement(1176..1709)
                     /gene="msrA"
                     /locus_tag="P5313_13270"
     CDS             complement(1176..1709)
                     /gene="msrA"
                     /locus_tag="P5313_13270"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRY81385.1"
                     /translation="MNTGEFETAVLGGGRFWCLEGAYEIVPGVVDVENGYAGGGRANP
                     TYEQVGTGLTGHAEVVKITFDPAKVTYTELLDLFWKIHDPTTLNRQGADIGPQYRSII
                     LYSSPEQEAAAKASIAKAAPSFRNPIVTELKPLETYWKAEGYHQDYFRRNPDQGYCQV
                     VVAPKVDKAQEWIRTNP"
     assembly_gap    1905..2004
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2254..2652
                     /locus_tag="P5313_13275"
     CDS             2254..2652
                     /locus_tag="P5313_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY81386.1"
                     /translation="MNSTMTERGIRFSLDAAGGVPFYRQIIQQIEQAILARRLEPGDR
                     LPTIRALAIDLKINPNTIAKAYGELEIRGIVTTQVGSGTYVSDKRIDMQEAERTKKLE
                     AAVARFIRDMEALGVDRTAMADLVLSFEED"
     gene            2655..3791
                     /locus_tag="P5313_13280"
     CDS             2655..3791
                     /locus_tag="P5313_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRY81387.1"
                     /translation="MWLFNKKVETPLIKNPRKPGRRRDFALSWAGAALLAALAAFALA
                     ARLLTGASPSLAAFGALLGAPVAAIIVMALFPRWNASIKLVILTAFALAVWRTGESGL
                     ILAFAAAGVLVAPAVQKAEEWERAIVLRFGKFHRVRGPGLFLLLPLADRVAQVVDLRI
                     RVTDFAAETTLSRDSVTVTVDALCFWMVWDAEKAVLEVQDYEEAVILSSKTALRAAVS
                     RNDLSAFLERGDEIENQIRAEVDQKTTEWGITVQHIEITDIQIPESLQGPLSRLAQAE
                     REKKGRILLAEAEIEIARKLEEAGKIYTANDLALKLKSLSILNEGLKAGNSMMLVPNY
                     ITEELKSKDLFGLQALTENHREGAGGGKKSAPDTGAGETDGTEK"
     gene            3863..4489
                     /locus_tag="P5313_13285"
     CDS             3863..4489
                     /locus_tag="P5313_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81388.1"
                     /translation="MALGAVDLDIEKGEYLAVVGPSGSGKTTLLNILGCLDVPTSGRV
                     VYDGRELRALGEKELSEYRKLHVSFIFQSYNLIPVLTVAENVELPLVIERKRNGAQAR
                     AKALELLSAVGLSGMENRYPRELSGGQEQRVAIARALVKDPIVVLADEPTANLDSHTA
                     EEIVELMKRINAERKTTFIFSTHDRLVMDHARRIVTLRDGAVTSDERR"
     gene            4491..5750
                     /locus_tag="P5313_13290"
     CDS             4491..5750
                     /locus_tag="P5313_13290"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY81389.1"
                     /translation="MNTLFKIAARNVLRNKRRTAITGIVMMFGIGLYIYADSILKGMD
                     RMTIDNMVNYTESFLKVRTPEYVSNLQGNPLEHGIPDPEATIRKILAADPTMKAAAPR
                     TRFVARLSNYEDEIPVLATAVDPVRDGEVLRLTDVVVQGVWLDKAEPRSVVIGADLAA
                     ELGLGLGDYVLVSASTVYENVNADEYRIAGLLAAPDPSLNKAALFMTFPDADEFLGLN
                     GLVTEIVTAGRAYPALDALLKGADGAAASVSKALPGLQADPIRVLAQDYLAMREMKGK
                     FSSAIILIMLVIAGVGIVNTILMAVYARIREIGVLRAYGMTAKEIRRLFTLEGLIVGA
                     VGSLAGVLLGVAIVAVTSTYGLSFAQIVGDVDMGGIPIMDRLYGEWNFGNMVFGFLFG
                     MFVSFLSARIPAKRAAKMQVTDALRFV"
     gene            5762..7006
                     /locus_tag="P5313_13295"
     CDS             5762..7006
                     /locus_tag="P5313_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY81390.1"
                     /translation="MRLWTMAVRNLGRNRRRSLLAGLSVLISILLILFMNGLMSGMMG
                     SIVKNFTKNDVGHLNIVTDGYRARERFMPVTEGIPDSAEVVGIVEAIPELKGRLLNTA
                     ERIRFGVLLSSGPNSKAALGIAGDPVTERSMLMLDRSVLPGGSYLTGPGQAILGEALA
                     RDLGLGVGDVLKVVTQKADYGLGFKRFTVAGIFKTNVNALDNAVFQIGIRDARELLGM
                     GDGAQQVLVMLKNYREADRVEALIDAALKKAGRDGLSVHSWTRMGDYPRFVRMAEGIY
                     MYMYVVVAFLGAFIITNIMMMVVLERRKEIGILKSMGMPKGDILKLFLLEGTLLGMAG
                     SAVGVVLGLGMNFALSKVGMDFSRSMAGFDWPMDNIVYPAVNPAMGLAFFALGSIVAA
                     TVSLLPSRSAARMNPIDAIRSV"
     gene            7008..7739
                     /locus_tag="P5313_13300"
     CDS             7008..7739
                     /locus_tag="P5313_13300"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRY81391.1"
                     /translation="MKRIFFVYLITLAAGAAFGQTAAEILRKVDENQAFQSIEYTGRM
                     EIVLGGDTRVKTMRTWAVGDSKAFVEFTNPEDRGTRMLKLDKNLWMYFPKEQDTVKIS
                     GHLLKEGMMGSDVSYEDAMESDALLDKYSASVLREEAVEGRPAWVLELKAKVPTATYD
                     RRLVWIDKERYVSLKGEMYARSGKLLKESRSLEVRNIGGRWYPVRTEMVNRLRKDTRT
                     VFVLEDLKLDTRIPDSRFSMAELTK"
     gene            7736..>8180
                     /locus_tag="P5313_13305"
     CDS             7736..>8180
                     /locus_tag="P5313_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81392.1"
                     /translation="MRILSRIAAYAGLVPAVAAVLLLTAASGSAQEPSWTLGGSLTQR
                     AFAAAAESAAGTDYSYGSATTLEATLRARGPVLRAEFSAEAALLTGASAEAFAAAVSA
                     LPPDTLYAPSAPAGAETVLAARVRTAYVKWTAEGFSLTAGRQIVNY"
BASE COUNT         1498 a         2590 c         2615 g         1377 t
ORIGIN      
        1 agtggatggc ctcggtgccg cggatctggg agtccgtcaa ggacggaatc taccgcaata
       61 tccgccagac cgggggcgtc aaggcggccc tcttccactt cttcgtggcc gtgggctccg
      121 cgttctccca tttccggaac cagctcctgg ggctcctgcc ggacttcggg aatcgtgtgc
      181 cgatcctgga ccggatccta ccgatcatcc ccctcgtact cctggcgccc ctgaacgcct
      241 tgggagggat cctggtcttc aagaagatca agcacaaact gggcggcaac ttcatagccg
      301 gcatctccgg cggcggcgcc ctgccctccg cggtggacca gttcttcggg gccctggggg
      361 tactgatcct ggaaggctac ggcctgacgg agacggcccc ggtcctgggc gttcggcccc
      421 agcacaagcc cgtcctggga accgtgggtc ccgctctcaa gggcacggag atcaagatcg
      481 tcggggaaaa cggcgtggtc ctgcccgccg gacagaaggg actcatcctg gtgaagggtc
      541 cccaggtcat gaagggctac tacaagcgcc cggacctgac ggagaaggtc ctctccgcgg
      601 acggctggct ggacacggga gacctgggca tgctgacccg cagcggagag atcaagatca
      661 ccggccgggc caaggacacc atcgtcctcc tgggcgggga gaacatagag ccgctgccca
      721 tcgagcagaa gctctgcgag agcgagtaca tcaagcagac catcgtcctg gggcaggaca
      781 agaagttcct ggcggccctc atcgtccccg acgcggacgc catcaacgcc tgggccaagg
      841 acaaccacgt ctacatcgtg gactacgaga ccctgctcca gcagcccgag gtccaggaac
      901 tcctggacta cgaggtcaag gagctgatct cccccaagaa cggcttcaag aacttcgagc
      961 ggatcttccg cttcaagctc ctgcccgtgc ccttcgaggt gggccgggag ctgtccgcca
     1021 agcaggaaat caagcgccac gtgatcaacg agatctacgc caaggacatc gcggcgctgt
     1081 tcaaggactg ataccaggca ttctaagaaa gatggaaagg ccgcgcaaac gcgcggcctt
     1141 tccatttccc ggggattcct aaccttgttt cgagcctagg gattcgtccg gatccactct
     1201 tgggccttgt ccaccttggg ggccacgacg acctggcagt atccctggtc ggggttgcgc
     1261 cggaagtaat cctggtggta cccctcggcc ttccagtatg tctccagggg tttgagctcc
     1321 gtcacgatgg ggttccggaa ggacggcgcc gccttcgcga tggacgcctt ggccgcggcc
     1381 tcctgttccg gactcgagta gaggatgatg gaccggtact gggggccgat gtcggctccc
     1441 tggcggttca aggtcgtcgg atcgtggatc ttccagaaga ggtccaaaag ctccgtgtag
     1501 gttaccttcg ccggatcgaa ggtgatcttg accacctcgg cgtgcccggt gaggcccgtg
     1561 cccacctgtt cgtaggtggg attcgcccgg ccgcccccgg cgtatccgtt ctccacgtct
     1621 acgacgcccg ggacgatctc gtacgccccc tcgaggcacc agaaacgccc cccgcccaga
     1681 accgcggtct cgaactcccc cgtattcatc acgcttcccc ccgattccac ggtcttcgcg
     1741 gcaggcgccc gattcgactc ggtcccagcc ccgacgcacg ataccgatag agcggtcgct
     1801 gccgcggccg cggctataaa cattctcata ttagtaatat ataatctgca ccaggacggt
     1861 tcaagtgcgg aacgtccagg attgaggata gaggaaccac cctgnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnaccagg acggttcaag tgcggaacgt ccaggattga
     2041 ggatagagga accaccctgt cggtggagcc aatttcaaaa gtcggtcaat tttaaacttg
     2101 gctttgcgat tgcttgcggt tgcgttgcag ccgctgggcc caatcacgga tttcaaggca
     2161 gctttttcaa aactccctga gttttgaaaa aggcctggta aaatcgaaaa acgcacttga
     2221 cacattgtac tagttcaaat agtattgtac tagatgaata gtacaatgac cgagcgaggc
     2281 atacgatttt ccctggacgc ggccggcggg gttcccttct accggcagat catccagcag
     2341 atcgagcagg ccatcctggc ccgccgactg gaacctgggg accgcctgcc gacgatccgg
     2401 gccctggcga tcgatctcaa gatcaacccc aacaccatcg ccaaggccta cggagagctg
     2461 gaaatcaggg gaatcgtcac cacccaggtg gggagcggga cctacgtctc ggacaagagg
     2521 atagatatgc aagaagcgga acggaccaag aagctggaag ccgccgtggc ccggttcatc
     2581 cgggacatgg aagcgctcgg ggtggacagg acggcgatgg cggatctggt cctgtccttc
     2641 gaggaggact gagtatgtgg ttgttcaaca agaaagtgga gacgcccctg atcaagaatc
     2701 cccgaaagcc cgggcgccgc cgggacttcg ccctttcctg ggcgggcgcc gccctgctgg
     2761 cggccctggc cgccttcgcc ctggccgccc ggctcctgac cggggcctcc ccctccctgg
     2821 cggccttcgg cgccctcctg ggcgcaccgg tcgcggcgat catcgtcatg gccctgttcc
     2881 cccggtggaa cgcctcgatc aagctcgtca tcctgacggc cttcgccctg gcggtctggc
     2941 gcaccgggga atccggcctg atcctggcct tcgcggccgc gggggtgctg gtagccccgg
     3001 cggtccagaa ggcggaggag tgggagcggg ccatcgtcct gcgcttcggc aagttccacc
     3061 gggtccgggg gcccgggctc ttcctgctcc tccccctggc agaccgggtg gcccaggtgg
     3121 tggacctgcg gatccgcgtg acggacttcg cggctgagac gaccctgtcc cgggattccg
     3181 tgaccgtgac cgtggacgcc ctgtgcttct ggatggtttg ggacgccgaa aaggccgtcc
     3241 tggaggtcca ggactacgag gaggcggtga tcctgtcttc caagaccgcc ctgcgcgccg
     3301 cggtcagccg gaacgacctg tccgccttcc tggagcgggg ggacgagatc gagaaccaga
     3361 tccgggcgga ggtggaccag aagactacgg agtggggaat cacggtccag cacatcgaga
     3421 ttacggacat ccagatcccc gagagcctcc agggccccct gagccgcctg gcccaggcgg
     3481 agcgggaaaa gaaggggcgg atcctcctgg cggaggcgga gatcgagata gcccggaagc
     3541 tggaagaggc ggggaagatc tacaccgcca acgacctagc cctcaagctc aagagcctgt
     3601 ccatcctgaa cgagggcctc aaggcgggca attccatgat gctcgtgccc aactacatca
     3661 cggaggagtt gaaatccaag gacctcttcg gcctgcaggc cctgaccgag aaccaccggg
     3721 aaggcgcggg gggcggtaaa aaatccgcgc cggacacggg tgccggcgaa accgacggaa
     3781 ccgagaagta aggaggacga tatggccatt ttgcacctgg aaaaaatacg gaaggtctat
     3841 aaaaaaggga gacaacgagg tcgtggccct gggcgccgtg gacctggaca tagaaaaggg
     3901 cgagtatttg gcggtcgtgg gaccctcggg ctcgggcaag accaccttgc tcaacatcct
     3961 gggctgcctg gacgtcccga cctccggccg ggtggtctac gacggccggg agctccgcgc
     4021 cctgggggag aaggagcttt cggaatatcg gaagctgcac gtctccttca tcttccagtc
     4081 ctacaatctc atccccgtgc tgacggtggc ggagaacgtg gaacttccgc tcgtgatcga
     4141 gcggaagagg aacggcgccc aggcccgggc caaggccctg gagctccttt ccgccgtggg
     4201 gctttcgggc atggaaaacc ggtatccccg ggagctctcc gggggccagg aacagcgggt
     4261 ggccatagcc cgggccctgg tcaaggatcc catcgtggtc ctggcggacg agccgacggc
     4321 caacctggat tcccacaccg ccgaggagat cgtggagctc atgaagcgca tcaacgcgga
     4381 gcggaaaacc accttcatct tctccaccca cgaccggctg gtcatggacc acgcccgccg
     4441 catcgtgacc ctccgggacg gggcggtcac gagcgacgag aggaggtgac atgaacaccc
     4501 tgttcaagat cgccgcccgc aacgtcctgc ggaacaagcg gcgcacggcc atcacgggta
     4561 tcgtcatgat gttcgggatc ggcctctata tctacgcgga ctccatcctg aagggcatgg
     4621 accggatgac catcgacaac atggtcaact acaccgagtc cttcctgaag gtccgcaccc
     4681 cggaatacgt gtcgaacctc cagggcaatc ccctggagca tggaatcccg gatcccgagg
     4741 ctacgatccg caaaatcctg gccgcggatc cgaccatgaa ggccgcggcg ccccggaccc
     4801 gcttcgtggc ccggctgtcc aactacgagg acgagatacc cgtgctggcc accgccgtgg
     4861 atccggtccg ggacggggag gtcctgcgcc tgacggacgt ggtcgtccaa ggggtctggc
     4921 tggacaaggc cgaaccccgc agcgtcgtga tcggggcgga tctggccgcg gaactgggac
     4981 tcggactggg ggactacgtc ctggtctccg catccacggt ctacgagaac gtgaacgccg
     5041 acgaataccg gatcgcgggc ctcctggccg ccccggatcc ctccctgaac aaggcggccc
     5101 tcttcatgac cttcccggac gcggacgagt tcttgggctt gaacggtctg gtcacggaga
     5161 tcgtgacggc cggccgggcc taccccgccc tggacgccct cttgaaggga gccgacgggg
     5221 cggcggcctc ggtttccaag gccctgccgg ggctccaggc cgaccccatc cgggtcctgg
     5281 cccaggatta tctggccatg cgggagatga aaggaaagtt ctcctcggcc atcatcctga
     5341 tcatgctggt gatcgccggg gtgggcatcg tgaacaccat cctgatggcc gtgtacgccc
     5401 gtatccggga gatcggggtc ctccgggcct acgggatgac cgccaaggaa atcaggcggc
     5461 tcttcaccct ggaaggcctg atcgtgggcg ccgtggggag cctggcgggg gtcctcctgg
     5521 gggtcgccat cgtggcggtc acctcgacct acggcctttc cttcgcgcag atcgtcgggg
     5581 acgtggacat gggcggaatc ccgatcatgg atagactgta cggagaatgg aacttcggca
     5641 acatggtctt cggattcctg ttcgggatgt tcgtgtcctt cctctccgcc cgcattcccg
     5701 cgaagcgggc cgccaagatg caggtcaccg acgccctgcg cttcgtctaa ggaggacagc
     5761 gatgcgacta tggaccatgg ccgtccggaa cctcgggcgc aaccggcgga ggagcctcct
     5821 ggcggggctc tccgtcctga tctcgatcct cctcatcctg ttcatgaacg gactcatgag
     5881 cgggatgatg ggatccatcg tcaagaattt cacgaaaaac gacgtgggtc acctgaacat
     5941 cgtgaccgac ggctatcggg cccgggaacg cttcatgccc gtgaccgagg gcatcccgga
     6001 ttccgccgag gtagtcggga tcgtggaggc catccccgag ctcaagggac gcctcctgaa
     6061 cacggccgaa cggatccgct tcggggtcct tttgtcctcg gggccaaact ccaaggccgc
     6121 cctgggcatt gccggggacc ccgtcacgga aagatccatg ctgatgctgg accgcagcgt
     6181 ccttcccgga ggatcctacc ttaccgggcc gggccaggcc atcctgggcg aggcattggc
     6241 ccgggacctg ggcctgggcg tgggagacgt ccttaaggtg gtcacccaga aggcggacta
     6301 cggcctggga ttcaagcgct tcaccgtggc ggggatcttc aagaccaacg tgaacgccct
     6361 ggacaacgcg gtcttccaga tcggcatccg ggacgcccgg gaactcctgg gcatgggaga
     6421 cggggcccag caggtcctgg tcatgctgaa gaattaccgg gaggcggacc gcgtggaagc
     6481 cctgatcgac gccgccctga agaaggcggg ccgggacggc ctatcggtcc attcctggac
     6541 ccggatgggg gactaccctc gcttcgtccg catggccgaa ggcatctaca tgtacatgta
     6601 cgtcgtcgtg gccttcctgg gggccttcat catcacgaac atcatgatga tggtggtcct
     6661 ggagcggcgc aaggaaatcg ggatcctcaa gagcatggga atgcccaagg gcgacatcct
     6721 gaagctcttc ctgttggagg gcaccctcct gggcatggcg ggatccgcgg tgggcgtggt
     6781 cctggggctg gggatgaact tcgccttaag caaggtcggc atggatttct cgaggtccat
     6841 ggcgggcttc gactggccca tggacaacat cgtctacccc gccgtgaatc ccgccatggg
     6901 gctggccttc ttcgccctgg gttcgatcgt ggcggccacg gtgtccctcc tgccctcgcg
     6961 cagcgcggcc cgcatgaatc ccatcgacgc gatacggagt gtgtaatatg aaacgaatct
     7021 tttttgtata cctgatcacc ctggccgccg gcgcggcctt cggccagacc gccgcggaga
     7081 tcctgcggaa ggtcgacgag aaccaggcct tccagtccat cgagtacacg gggaggatgg
     7141 agatcgtcct ggggggtgac acccgggtca agaccatgcg gacctgggcc gtaggggaca
     7201 gcaaggcctt cgtcgagttc acgaaccccg aggaccgggg gacccggatg ctcaagctgg
     7261 acaagaacct ctggatgtac ttccccaagg agcaggacac tgtgaagatc tccggtcacc
     7321 tgctcaagga ggggatgatg ggcagcgacg tctcctacga ggatgccatg gagtcggacg
     7381 ccctcctgga caagtactcc gcctcggtcc tgcgggagga ggccgtggag ggccggcccg
     7441 cctgggtcct ggaactgaag gccaaggtcc cgacggccac ctacgaccgc cgcctggtct
     7501 ggatcgacaa ggagcgctac gtgagcctca agggcgagat gtacgcccgg agcggcaagc
     7561 ttctcaagga gagccgctcc ctggaggtcc ggaacatcgg cgggcgctgg tacccggtcc
     7621 gcacggagat ggtcaaccgg ctgcgcaagg acacccggac ggtcttcgtc ctggaggacc
     7681 tgaagctcga cacccgcatc ccggattccc ggttcagcat ggcggagctt acgaagtgag
     7741 gatcctatct agaatcgcgg cgtacgcggg cctcgtcccg gccgtcgccg cggtcctcct
     7801 cctgacggcc gcctcgggct cggcccagga gccgtcctgg accctgggcg gctcccttac
     7861 ccagcgggcc ttcgcggctg cggcggagtc ggcggccggc acggactatt cctacggctc
     7921 ggcgaccacc ctggaggcga ccctgcgggc ccggggcccg gtcctccggg cggagttctc
     7981 cgccgaggcc gccctgctga ccggagcctc cgcggaggcc ttcgcggcgg ctgtctcggc
     8041 cctccccccg gacaccctgt acgccccctc ggcccccgcg ggtgcggaaa ccgtactggc
     8101 cgcccgggtc cgcaccgcct acgtcaagtg gaccgccgag ggtttctccc tgaccgcggg
     8161 ccggcagatc gtcaactacg
//
LOCUS       DAOTEI010000159         8004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6381_length_8004_cov_5.238646, whole genome shotgun sequence.
ACCESSION   DAOTEI010000159 DAOTEI010000000
VERSION     DAOTEI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6381_length_8004_cov_5.238646"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="P5313_13310"
     CDS             <1..1530
                     /locus_tag="P5313_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY81393.1"
                     /translation="SNTTETHPVIGTRIKRAVRRGARLIVSDPRRIELAEEAEIFLPL
                     KPGTNVALYNALACAILEEGLADREFIAERTEGFEEWETSVRSCTPEKAAEICGVDAG
                     DIRRAARIYAGARAAGIYYAMGVTQHTAGTESVMALSNLALACGKLGKAGCGINPLRG
                     QNNVQGACDVGALPDVLPGYRKVSDPAARAAAAAVWGREPPAEPGLTVTEMIRAAETG
                     HIGFLYVMGENPLVSDPDIGHVREALQAAEFLVVQDIFLTETAALADVVLPAACFAEK
                     DGTFTNTERRVQRVRKAIPPPGRAREDLDILAELLARLGLPQADRTGAGVFAELARLA
                     PQYAGMSWGRLEDGGLQWPCPAPDHPGTPILHVGRFTRGPGRFIPYRWRPPAEEPDAE
                     YPLVLTTGRNLYQYHTRTMTGREEGLSILAGEAYAELHPEAAVRAGVRHGEILRLSTR
                     RGSIELTARVSEGIRPDTVFVPFHYAEAAANILTGTALDPHAKIPELKVCAVRAERGG
                     A"
     gene            complement(1537..2553)
                     /locus_tag="P5313_13315"
     CDS             complement(1537..2553)
                     /locus_tag="P5313_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017788543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81394.1"
                     /translation="MKQFWTMVYARTMEFVRDRGTFFWNLLFPVLLVFGFSFAFSGEG
                     QPLFKVGVLDSSPAALEGPASFLSIEQGRFIVYGPEESRTAVLDRLRRHELDMVLDPA
                     DRSYYLNVQGRTASLLRRLAAADPVLRGYLERTVSGAPIRYVDWLVPGVIGMNIMFSC
                     LFGVGFVLVRYRKNGVLKRLKATPVSAAAFVTAQAVSRFLIVILTSVVVFAGTNAFLR
                     FRMEGSWAALLAIASLGIVSMEALGLVFAARLRSEELVNGLMNLATLPMLALSGVFFS
                     LEGSPEILKTVSRALPLTHAIGAARAVMLEGAGLGRIAPDLLFLGLFSAAALALASAL
                     FRWE"
     gene            complement(2700..3572)
                     /locus_tag="P5313_13320"
     CDS             complement(2700..3572)
                     /locus_tag="P5313_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81395.1"
                     /translation="MATILEAAGLRKRYGSVQAVDGVSLPIEEGTCLGILGPNGAGKT
                     TTIEMMEGILAPDEGTVTFRGEPIGREFRRKVGIQFQTTALPEFITVRETLKLFSSFY
                     PSPRPFDEVVDLCSLSDILDRDNRKLSGGQRQRMLLALAIAPRPEVVFLDEPTTGLDP
                     QARRNFWDLIRGIRAERTTVVLTTHYMDEAQTLCDEVAIMDRGRVLERGNPARLLARH
                     FRGALVRIPRGCVEGDGIPGKLVADHLEIQSENLDRTMRELLAAGVNLEGLSIHKPDL
                     EDLFIELTGSSLRN"
     gene            3685..4110
                     /locus_tag="P5313_13325"
     CDS             3685..4110
                     /locus_tag="P5313_13325"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRY81396.1"
                     /translation="MLDLSVAFLPLAAIALANFFLSWLYYSPAAPWFRTWAAGVGMDP
                     DRREMSEEDRRAMPRLMGGAVVASVLLSYGLQVFVRSVRAETFGQGALTGIALWAAFA
                     VTQSLNTQFEGRKPSVLIINNALYLLTYAVFGGILAVWR"
     gene            complement(4213..5346)
                     /locus_tag="P5313_13330"
     CDS             complement(4213..5346)
                     /locus_tag="P5313_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY81397.1"
                     /translation="MPGLLPDVDPDGLLEYSVVYTDRSVNHMSKLFQGALRDISRVMK
                     EVYKARSAVIVPGSGTFGMEAVARQFATGKKCLVIRNGLFSFRWSQIFEVGKIPSSET
                     VLKARRTAEAPESPFAPVPIAEAVETIRREKPDLVFAPHVETSSGMILPDGFIKSLAD
                     AVHAHGGMLVLDCVASGAIWVDMKALGIDILVTAPQKGWSGQPCCGMVALGETARQRI
                     DSTVSTSFSCDLKKWLEIMETYEAGKHAYHATPPTDSLLVLRDVMKEMEAFGFEKART
                     AQAELGRRVRTLLASRGFKSVAAPGFEAPGVVVSYTTDPDIQSGKKFLDLGMQVAAGV
                     PIKCDEPADYKSFRVGLFGLDKLGNLERTVKTFEATLDKVLGR"
     gene            complement(5419..5910)
                     /locus_tag="P5313_13335"
     CDS             complement(5419..5910)
                     /locus_tag="P5313_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81398.1"
                     /translation="MSKRIRKFGSLSAFLLALAVVFVPVPAVAQGGSRLAVTAPAGWS
                     PHPSNASMMMKGTGTYTLKPENMPSEANTPDSFTAFAQKNFAKSFSNCKFSTVTSLKV
                     SGNEARCFEFTGEVSGMKMTYLVLYVFQGGRAHSLVCGSLADGFAALKPDYEKVIASA
                     RME"
     gene            complement(6083..7498)
                     /locus_tag="P5313_13340"
     CDS             complement(6083..7498)
                     /locus_tag="P5313_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRY81399.1"
                     /translation="MAKEKNRRFPHVYVILFIVIIAAALLTYVLPANQYDRQIKDGKE
                     TKLIDPNSYHPIDPNPVDPWETLLAIPKGLQEVASIIFFIFIVGGSFNIIQSTGAVEA
                     GIRSLVNALGGKGSGGAWKDGMLLFLIVAAFSFGGSSFGMAEEALVFIPMLVPLAIAM
                     GYDSLTGLALALVGPCAGFTGAFINPFTLQVAQGFVGLPLLSGMGYRIIIFSVQTILA
                     FLFIFWYAQRVKKNPKLSVMYEADQKREKIDLDAKSPFTGRQKLVLVFMVACFALLIY
                     GVNKWGWYIDEIAALFLAMGAISGFIGGKGPSKVAEAFVEGSTALTVGALVVGVARAI
                     LVVLTQGGILHTIVHAMAAAVQGLPPFLSAIGMYVVQILISVIIPSGSGMAAVTMPIM
                     GPLATLLGMTQQTAVLIYQFADGFTNIIIPTSGYLLAGVALAKVPYEKWVKWYAPLFG
                     IFLAVGAVFVVIAQAIAFGPF"
     gene            complement(7929..8001)
                     /locus_tag="P5313_13345"
     tRNA            complement(7929..8001)
                     /locus_tag="P5313_13345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7966..7968),aa:Met,seq:cat)
BASE COUNT         1338 a         2603 c         2754 g         1309 t
ORIGIN      
        1 tccaacacca ccgagaccca tcccgtcatc ggtacccgga tcaagcgggc cgtccgacgc
       61 ggggcgcgcc tgatcgtgtc ggacccccgc cgcatcgagc tggccgagga ggcggagatc
      121 ttcctgcctc tgaagcccgg caccaacgtg gccctctaca acgccctggc ctgcgccatc
      181 ctggaggagg gcctggcgga ccgggagttc atcgccgagc ggaccgaggg cttcgaggag
      241 tgggaaacct ccgtccggtc ctgcaccccc gagaaggccg ccgagatctg cggggtggac
      301 gccggtgaca tccggcgggc ggcccggatc tacgccgggg cccgggccgc ggggatctac
      361 tacgccatgg gggtcaccca gcacaccgcc ggcaccgagt ccgtcatggc cctgtccaac
      421 ctggccctgg cctgcggaaa gctcgggaag gccggctgcg ggatcaaccc cctgcggggc
      481 cagaacaacg tccagggagc ctgcgacgtc ggggccctgc cggacgtcct gccgggatac
      541 cggaaggtct ccgaccccgc ggcccgggcc gcggccgcgg cggtctgggg ccgggagcct
      601 ccggcggagc cggggctgac ggtgaccgag atgatccggg ccgccgagac ggggcacatc
      661 ggcttcctct acgtcatggg ggagaatccc ctggtctccg acccggacat cggccacgtc
      721 cgggaggccc tgcaggcggc ggagttcctg gtggtccagg acatcttcct aaccgagacg
      781 gcggccctgg cggacgtggt ccttccggcc gcctgtttcg cggaaaagga cggcaccttc
      841 acgaacaccg aacggagggt ccagcgggtc aggaaggcga tcccgccccc ggggcgggcc
      901 cgggaggacc tggacatcct ggcggagctc ctggcccggc tgggcctgcc ccaggcggac
      961 cggacggggg cgggtgtctt cgcggagctg gcccgcctgg cgccccagta cgcggggatg
     1021 tcctggggcc ggctggagga cgggggcctg cagtggccct gccccgcccc ggaccatccg
     1081 ggcaccccca tcctccacgt cgggcgcttc acccggggcc cgggacgctt catcccctat
     1141 cgttggcggc cgcccgcgga ggagcccgac gccgaatatc ccctggtcct gaccacgggt
     1201 cggaacctct accagtacca cacccggacc atgaccggcc gggaagaggg gctctcgatc
     1261 ctggccggtg aggcctatgc ggagctccac ccggaggccg cggtccgggc cggagtccgg
     1321 cacggcgaga tcctgcggct ttccacccgg cggggctcca tcgagctgac cgcccgggta
     1381 tccgagggga tccgcccgga cacggtcttc gtgcccttcc actatgccga ggccgcggcg
     1441 aacatcctga ccgggaccgc cctggatccc cacgccaaga tcccggagct caaggtctgc
     1501 gcggtccggg cggagcgggg cggggcctga tcctcctcac tcccaccgga agagggcgga
     1561 ggcgagggcc agggctgccg cgctgaagag ccccaggaac agcaggtccg gggcgatcct
     1621 gcccaggccg gccccctcca gcatgaccgc ccgggcggct ccgatcgcgt gggtcaaggg
     1681 aagggcccga gatacggtct tgaggatctc cggggaaccc tccagggaga agaacacccc
     1741 ggacagggcc agcatgggca gggtcgcgag gttcattagg ccgttgacca gctcctcgct
     1801 ccggagccgc gcggcgaaga cgagccccag ggcttccatg ctgacgatcc ccagggaggc
     1861 gatcgccagg agggccgccc aggacccctc catacggaac cggaggaagg cgttcgtgcc
     1921 cgcgaagacg acgacgctgg tcaggatgac gatgaggaac cgggatacgg cctgggcggt
     1981 cacgaaggcc gcggcggaca cgggggtcgc cttcaggcgc ttgagcaccc cgttcttccg
     2041 gtaccgaacc aggacgaagc cgacgccgaa gaggcaggag aacatgatgt tcatcccgat
     2101 gacccccggg acgagccagt ccacgtaccg gatcggggca ccggagaccg tccgttcgag
     2161 gtagcctcga agcacgggat ccgcggccgc cagcctgcgc aggaggctgg cggtccggcc
     2221 ctggacgttc aggtagtagc tccggtccgc gggatccagg accatgtcca gctcgtgccg
     2281 ccggagcctg tccaggaccg ccgtgcggct ttcctccgga ccgtagacga tgaaccggcc
     2341 ctgctcgatg gacaggaagg acgccgggcc ttccagggcc gcgggggaag agtccaacac
     2401 tccgaccttg aagaggggct ggccctcccc ggagaaggcg aaggaaaacc cgaacacgag
     2461 gaggacggga aacaggaggt tccagaagaa ggttccccgg tcccggacga actccatcgt
     2521 gcgggcgtac accatggtcc agaattgttt catcgcgcgc cttcctagca gggtgctgac
     2581 aaactcgaag cttgtcagca ccctgctatg caattgctcg aaaagcttgc gcttttctaa
     2641 gcaattgcga cattaatgaa gctgttgaca aaggcgccat cgcctttgtc aacagcctgc
     2701 tagttccgca gggacgatcc cgtgagctcg atgaacaggt cctcgaggtc gggcttgtgg
     2761 atggacaatc cctccaggtt cactcccgcg gccaggagtt cccgcatcgt ccggtccagg
     2821 ttctcggact ggatctccag gtggtccgcg accagcttgc ccggaatccc gtccccttcc
     2881 acgcagcccc gggggatgcg caccagggct ccccggaaat gccgggctag gaggcgggcg
     2941 gggttcccgc gctccaggac ccttccccgg tccatgatcg ccacctcgtc gcagagggtc
     3001 tgggcctcgt ccatgtaatg ggtggtcagg accacggtgg tccgctccgc ccggataccc
     3061 cggatcaggt cccagaaatt ccggcgcgcc tggggatcga ggcccgtggt cggctcgtcc
     3121 aggaaaacca cctccggccg gggggcgatg gccagggcca ggagcatccg ctggcgctgg
     3181 ccgccggaca gtttacggtt gtcccggtcc aggatgtccg agagggagca caggtccacc
     3241 acctcgtcga aggggcgggg ggaggggtag aaggaggaga acagcttcag ggtctcccgg
     3301 acggtgatga actccggaag ggccgtggtc tggaactgga tccctacctt ccgcctgaat
     3361 tccctcccga tcggttcgcc ccggaacgtg acggttccct cgtccggggc gaggattccc
     3421 tccatcatct cgatggtggt ggtcttgccc gcgccgttcg ggcccaggat gcccaggcag
     3481 gtcccttcct cgatgggaag ggacacgccg tccacggctt gcacggagcc gtaccgtttc
     3541 cggagccccg cggcttcaag gatggtcgcc atggttcgaa ggtagcgatc ggccggccgc
     3601 ggggcaacgg aaaccgcgcg gtcgcgaggg ggatttgcct ttttatgagt aaaatcgtaa
     3661 ttttaacacc acaaggagcc ctacatgctt gacttgagtg tcgcctttct tcccctggcg
     3721 gcgatcgcgc tggccaactt cttcctttcc tggctctatt actcccccgc cgctccctgg
     3781 ttccggacct gggcggccgg cgtcgggatg gatcccgacc gccgggagat gtccgaggag
     3841 gaccgcaggg cgatgccccg gctgatgggt ggggccgtgg tcgcctcggt cctgctttcc
     3901 tacggcctgc aggtcttcgt acgctccgtc cgggcggaga ccttcgggca gggtgcgctg
     3961 accgggatcg cgctctgggc ggctttcgcg gtgacccaga gcctcaacac ccagttcgaa
     4021 ggccggaagc cctcggtcct gatcatcaac aacgccctct acctgctcac gtacgccgtc
     4081 ttcgggggca tcctggccgt ctggcggtag gttccccgct tccggtccgg gcggcgcgcc
     4141 tcttcgaccc gcggatcctc aagtggaacg gccgcccgtt tcggggcggc cgttccatct
     4201 ttggaagctc tctcagcggc cgagtacctt gtccagggtc gcctcgaagg tcttgaccgt
     4261 tcgctccagg ttccccagct tgtccaggcc gaacaggccc acccggaagc tcttgtagtc
     4321 cgcgggctcg tcgcacttga tgggcacccc ggcggccacc tgcatcccca ggtccaggaa
     4381 cttcttcccc gactggatgt ccggatccgt ggtatagctg accaccactc ccggggcctc
     4441 gaaacccggg gccgctacgc tcttgaagcc cctggaggcc aggagggtac gtacccggcg
     4501 cccgagttcc gcctgggcgg tccgggcctt ctcgaatccg aaggcttcca tttccttcat
     4561 cacgtcccgg aggaccagga gggagtccgt gggcggcgtg gcgtggtagg cgtgcttccc
     4621 ggcctcgtag gtctccatga tctccagcca cttcttgagg tcgcaggaga aactcgtgct
     4681 gaccgtggag tcgatgcgct gtcgggcggt ctcgcccagg gccaccatgc cgcagcaggg
     4741 ctggccgctc caacccttct ggggggctgt caccaggatg tcgattccca gggccttcat
     4801 gtccacccat atggcgccgg aggccacgca gtccaggacc aacatgcccc cgtgggcgtg
     4861 cacggcatcc gccagggact tgatgaagcc gtccggaagg atcatgcccg acgaggtctc
     4921 cacgtggggg gcgaagacca ggtccggttt ctcccgccgg atcgtctcga ccgcctccgc
     4981 gatcgggacc ggggcgaagg gggactccgg ggcctccgcg gtccggcgcg ccttgaggac
     5041 ggtctcggag gaggggatct tcccgacctc gaagatctgg ctccaccgga aactgaacag
     5101 gccgttccgg atgaccaggc acttctttcc cgtggcgaac tggcgggcca cggcctccat
     5161 gccgaaggtt ccggacccgg gcacgatgac cgcggagcgg gccttgtaga cctccttcat
     5221 gacgcgggag atgtcccgta gggcgccctg gaagagcttg gacatgtggt tcacggagcg
     5281 gtcggtgtag acgaccgagt attcgagaag accgtcggga tcgacgtcgg gcaagagtcc
     5341 gggcatggga gcctcctaga acagggatat acccgagggt accccgggag ggctcggcgg
     5401 cgcaaggtgc gccgcggtct actccatccg ggccgaggcg atgaccttct cgtagtccgg
     5461 tttcagggcg gcgaacccgt ccgccaggga tccgcagacc agactgtggg ccctgccgcc
     5521 ctggaagacg tacagcacga ggtaggtcat cttcatgccg gacacctccc cggtgaactc
     5581 gaagcaccgg gcctcgtttc cgctgacctt gaggctcgtg acggtactga acttgcagtt
     5641 ggaaaagctc ttggcgaagt ttttctgggc gaaggctgtg aaggagtccg gggtgttcgc
     5701 ctcggacggc atgttctccg gtttgagggt gtaggtgccc gtgcccttca tcatcatgga
     5761 ggcgttggag ggatgcgggc tccatccggc cggggcggtg acggccagcc gggagcctcc
     5821 ctgggcgacg gcggggacgg gaacgaagac gacggccagg gcgaggagga aggcggacag
     5881 ggaaccgaat ttgcggatac gtttcgacat gatgacctcc ggtggatgcg gtgcttttgg
     5941 gcggtctcgg cggccgccga ggagcatcgt atcccgtctc gcgcgccttg tcagcctcgc
     6001 cgcgtcgggg gtaaaaaaaa cgcccccgcg gcgggcgggg gcgaggctcg ctcggcgggg
     6061 aaccggtccc cgccgcgagg ggtcagaacg gtccgaaggc gatggcctgg gcgatgacga
     6121 cgaacaccgc gccgacggcc aggaagatgc cgaacagggg ggcgtaccac ttcacccact
     6181 tctcgtaggg caccttggcc agggccacgc ccgccaggag gtagcccgag gtcgggatga
     6241 tgatgttcgt aaaaccgtcg gcgaactggt agatcagcac cgcggtctgc tgggtcatgc
     6301 ccaggagggt ggccaggggg cccatgatcg gcatcgtcac cgccgccatg ccggagccgg
     6361 aggggatgat cacggagatg aggatctgca cgacgtacat cccgatcgcg gagaggaagg
     6421 gcgggagccc ctgcacggct gcggccatgg cgtggacgat ggtgtggagg atgcctccct
     6481 gggtcaggac gaccaggatg gcccgcgcca cgccgaccac gagggcgccc acggtaaggg
     6541 cggtcgagcc ctccacgaag gcctcggcga ccttgctggg acccttgccg ccgatgaagc
     6601 cgctgatcgc gcccatggcc aggaagaggg ccgcgatctc gtcgatgtac cagccccact
     6661 tgttgacgcc gtagatgagg agggcgaagc aggccaccat gaagaccagg accagcttct
     6721 gccggcccgt gaacgggctc ttggcgtcca ggtcgatctt ctcgcgcttc tggtccgcct
     6781 cgtacatgac gctgagcttg ggattcttct tgaccctctg ggcgtaccag aagatgaaga
     6841 ggaaggccag gatcgtctgc acggagaaga tgatgatccg gtatcccatg cccgagagga
     6901 ggggcaggcc cacgaagccc tgggccacct ggagggtgaa ggggttgatg aaggcgcccg
     6961 tgaacccggc gcagggtccg accagggcca gggccaggcc ggtcagggag tcgtagccca
     7021 tggcgatggc caggggcacg agcatcggta tgaaaacgag ggcttcctcg gccatgccaa
     7081 agctgcttcc tccgaaggag aaggctgcca cgatcaggaa gaggagcatt ccgtccttcc
     7141 aggcgccccc ggatcccttg cctcccaggg cgttcaccag gctgcggatg cccgcctcca
     7201 cggctcccgt ggactggatg atgttgaagg aacctccgac gatgaagatg aagaagatga
     7261 tgctggccac ttcctgcagg cccttcggga tcgccaggag tgtctcccag gggtcgacgg
     7321 gattgggatc gatgggatgg tatgagttgg gatcgatgag cttggtctcc ttgccgtcct
     7381 tgatctgcct gtcgtactgg ttggccggga gcacgtaggt gagcagggcg gccgcgatga
     7441 tcacgatgaa caggatcacg tagacgtgcg ggaaccggcg gttcttctcc tttgccatga
     7501 tctcgtctcc ttcgcagggc gcacgaactt caagcggctc caggagccgc tcgtcgcacg
     7561 gcgcattcca ggagcctcgc tccggtcgtt tgttcgtagg gccggcgttc ggggagggat
     7621 gcgctggggc gattcgatta tacgcccccc cggcgggaag tcaatcctgt atgtcgaata
     7681 atgaaacaag ccggccgacc tgtgaatgga tcggccggct tttggcaggg gtaggaccgt
     7741 tcgagtccta cccctgtggt aggactcgcc cttcattcga cggcttcgtg ccgtctcagt
     7801 ccgggccggg gttccggcct gtcggggcct tccatggccc ctttccctcg ctgccgctcg
     7861 gcggccggaa cccttcgagt ccgaaagata aaaagaaagc cggtccatag aaggaccggc
     7921 tttcttttta gcaggggtag gactcgaacc tacgacctcc gggttatgag cccgacgagc
     7981 tgccaactgc tctaccctgc gtcg
//
LOCUS       DAOTEI010000160         7978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6413_length_7978_cov_4.512180, whole genome shotgun sequence.
ACCESSION   DAOTEI010000160 DAOTEI010000000
VERSION     DAOTEI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6413_length_7978_cov_4.512180"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="P5313_13350"
     CDS             <1..459
                     /locus_tag="P5313_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81400.1"
                     /translation="GSAPGKSVAIGEIPIAIGYAHDQVKLKVEGAPVVINAPSEGTGY
                     ELASMSLIKGGKDAVNAKKLYDWVLSSKSESFFALAKKLYDWVLSSKSAQDLFVQWYL
                     VLVATGAPSHPDALSIKQIKTVKQDLLWDGNKDNRARLLKRWTDEIGSKR"
     gene            571..2769
                     /locus_tag="P5313_13355"
     CDS             571..2769
                     /locus_tag="P5313_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY81401.1"
                     /translation="MPKKTTILRFLAALALFAAVDAWVYFTLRGTLRSQEARDSESRL
                     TLLARSAPADPENLDTWLASVPESLPGGAAVYLIPTEDPEAYETRSSDEEAARLWDSV
                     SGGADAAKGLESAYYFENYASPQRVEYGGQRVRLFYAPVLDESESELLGIAVFLAPEA
                     GNRTLERLFALFAVGTLVLYAALSGISRFARDPTVGYAVVILAGIAGVFIAYPLAEAL
                     RLSFLKEGRFSLEIWKDVLSGAHLQALWGSVRLGIWTASVSTVIGFVFAFAIHRTGIG
                     GKKFLSTIAQLPVISPPFSLTLSVILLAGNNGLITRQLLGLQNFSIYGLSGLVIVQTV
                     GMFPIAYLTLAGVLQAMDSTLEEAALDLNAGRWRAFRTVTLPLAVPGILSAWLLVFTN
                     SLADFANPLLLAGNYRVLSVEAYIQVTGRNDLGGGAALSLLLLLPTLTAFFLQRNWVA
                     KRSFVTVTGKPNAKLVEIASPRVRFALGAGVWTCVALILGLYGTIVAGCFVKNWGIDY
                     SFTLSNIGEALTRGRDAIRSTVTLAAAATPVAGLLAMAAALVFVRKTFAGKRVLEALM
                     MTPFAVPGTLLGIAYILAFNKAPLILVGTSAIIVINYVIRELPVGIESGVASLRQIDP
                     SIEEAAKDLGADEGTVFRTIVLPLVRPAFITSMSYTFVRSMTAVSAVIFLISARWYHM
                     TVLIYNFSENLRFGLASVLATVLIVIVLGAFALMRLLVRDTGALEKTITR"
     gene            2788..3888
                     /locus_tag="P5313_13360"
     CDS             2788..3888
                     /locus_tag="P5313_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81402.1"
                     /translation="MAKQPVAVTLDDVTRVYKNVKGRPDVVAVRQASFEVAPGELVTL
                     LGPSGCGKTTTLRMIAGFDLPSSGLIRIGGEDVTNLPPNKRDTATVFQSYGLFPHMTV
                     AENVAYGLRVRNVPKAEQEERVRRALSLTGLSDYAERSPGRLSGGQQQRVALSRSLVV
                     EPGVLLLDEPLSNLDALLREQMRVEIRRIQKSLGITAVYVTHDRTEAMSLSDRVIVMK
                     DGRIRQIGSPSTIYEDPDSVFVAGFVGKVAFFPVAVEGLEGPGCACVLHGKTVRAGKR
                     APGVAAGKPGILMARPESLRLGAPGQGVADGTVRQNVYLGSSVETFVTTEYGELLVQV
                     DDPAGKRIPAEGEAVSISFNEARVRVLPPETE"
     gene            3893..4468
                     /locus_tag="P5313_13365"
     CDS             3893..4468
                     /locus_tag="P5313_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY81403.1"
                     /translation="MGTELDAIISSSVARIAGMRKPRELREGARYHVTGRANRKELIF
                     DTGAMKDLFLSVVKRAKRKYAFRIENFCIMGNHFHLVIIPGKGMSLSTIMQWIMSVFA
                     MAYNRICGYTGHVWGERFFSRILSGFRELLAVFAYIDNNPVQASLIEDPRKWRFGGLW
                     HNRTGCRDIVEVEPSWLPLTFPERGMLLLTN"
     gene            4806..6449
                     /gene="pruA"
                     /locus_tag="P5313_13370"
     CDS             4806..6449
                     /gene="pruA"
                     /locus_tag="P5313_13370"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRY81404.1"
                     /translation="MNDAILSAPPAGNEPVRSYAPGTPERAALKAELGSLLSESFDIP
                     LIIGGRELRTGRTAPLAQPHDHGRPLGRFHLAGEEEAALAVDAALAAKPGWEAMPWEE
                     RAAVFRRAAALVAGKYRYRLLAATMLGQSKTVHQAEIDAVCETADFLRINPRFMEELY
                     SIQPRSSDSEWNRVRYRPLEGFVYAVSPFNFTSIAANLASAPAMMGNVVVWKPASTSV
                     LSNWILARIYEEAGLPPGVINFLPGSGAAVSAVALSHPAFAGLHFTGSTAVFNGLWRQ
                     AAANLGSYRSYPRLVGETGGKDFVFLDPSADPDAAVAALVRGAYEYQGQKCSAASRLY
                     VPASLGRAFLDRLAETVRSIPVGPPTDLRAFMCAVIDGASYRNILPYLERACSRGAAR
                     CELELLAGGHGRAEEGWFIEPSLLLAESPDYETMREELFGPVLTAYVYDDRDMEAAYR
                     LVDGTSPYALTGAVFARDRRAILAGLEGLKNAAGNLYVNDKPTGAIVGSQPFGGMRAS
                     GTNDKAGSILNLIRWTSAGAVKENFDPPVDWRYPYMSEE"
     gene            complement(6832..7677)
                     /gene="ugpE"
                     /locus_tag="P5313_13375"
     CDS             complement(6832..7677)
                     /gene="ugpE"
                     /locus_tag="P5313_13375"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HRY81405.1"
                     /translation="MTEHSRFRRVLPHVFLILAVMVILFPIWLVFTASTRTNAEILSA
                     PMPISPGPHFLENYRRAILEGSANMGASALTMLKNSFIMAMGIAVGKIVVSLLAAYAI
                     VFFDFKLRAFCFWSIFITLMLPVEVRIMPTYKVISDLGLLNTYAGLILPMVVSATAVF
                     LFRQFFLSVPREIAEASQIDGAHAMQFFRRILVPMTRTPIAAMFVIQFIYGWNQYLWP
                     LLITTKQEYYTLLIGINRMLQGADVLIEWQIVMATTMLALIPPALVVVVMQKQFIAGM
                     TDTEK"
     gene            complement(7690..>7978)
                     /locus_tag="P5313_13380"
     CDS             complement(7690..>7978)
                     /locus_tag="P5313_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008558267.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
                     /protein_id="HRY81406.1"
                     /translation="ARRFWKIIFPILSPTSFYLLVMNLVYGFFETFPIVHQVTAGGPG
                     KSTAILVYKVWRDGVVNLDLGGSSAQSVILMLMVIGLTIVQFRFIERRVNY"
BASE COUNT         1314 a         2689 c         2638 g         1337 t
ORIGIN      
        1 ggctcggccc ccggcaagag cgtggccatc ggggagatcc ccatcgccat cggctacgcc
       61 cacgaccagg tcaagctcaa ggtcgagggc gcccccgtcg tgatcaacgc cccctccgag
      121 ggcaccggct acgagctggc ctccatgtcc ctgatcaagg ggggcaagga cgcggtcaac
      181 gcaaagaagc tctacgactg ggtgctctcc tccaagtccg agagcttctt tgcgttggcc
      241 aagaagctct acgactgggt gctctcctcc aagtccgccc aggacctctt cgtccagtgg
      301 tacctggtcc tggtggccac cggcgccccc tcccacccgg acgccctgtc catcaagcag
      361 atcaagaccg tcaagcagga cctcctgtgg gacgggaaca aggacaaccg ggcccgcctg
      421 ctgaagcgct ggaccgacga gatcggctcc aagcggtaaa cccccgttcg gcccggcacc
      481 ggaccggccg ccccgcgggg ccggcccggc tccggccgac gccggcggcc gctcccggcc
      541 gccgggcccc acgctccgac gggagacccc atgcccaaaa aaacgacgat cctgagattc
      601 ctggccgccc tggccctgtt cgcggcggtg gacgcctggg tgtacttcac cctccgcggg
      661 accctgcggt cccaggaagc ccgggattcc gagagccgcc tgaccctcct ggcccgcagc
      721 gcccccgcgg atcccgagaa cctggacacc tggctggcct cggtgccgga aagcctgccc
      781 ggcggcgccg cggtctatct gatacccacc gaggatccgg aggcctacga gactcggagc
      841 tccgacgagg aagccgcccg cctgtgggac tccgtgtccg gcggtgcgga cgcggccaag
      901 ggcctggagt ccgcgtacta cttcgagaac tacgcctccc cccagcgggt ggagtacggg
      961 ggacagcggg tccggctctt ctacgccccg gtcctggacg aaagcgagtc cgagcttttg
     1021 gggatagccg tgttcctggc ccctgaggcc gggaaccgga ccctggagcg gctcttcgcc
     1081 ctcttcgcgg tggggaccct cgtcctctac gcggccctgt ccgggatctc ccgcttcgcc
     1141 cgggacccga ccgtgggcta cgccgtcgtg atcctggcgg ggatcgccgg ggtcttcatc
     1201 gcctaccccc tggccgaggc cctccgcctt tcgttcctga aggaggggag gttctccttg
     1261 gagatctgga aggacgtcct ctcgggcgcc cacctgcaag ccctctgggg ttccgtgcgc
     1321 ctggggatct ggaccgcctc ggtgtccacg gtcatcggct tcgtgttcgc cttcgcgatc
     1381 caccggaccg gcatcggcgg caaaaaattc ctgtccacca tcgcccagct tccggtgatc
     1441 tccccgccct tctccctgac tctctcggtg atcctcctgg cggggaacaa cggcctcatc
     1501 acccgtcagc tgctgggcct ccagaacttc tccatctacg gcctgtcggg cctggtgatc
     1561 gtccagaccg tggggatgtt ccccatcgcc tacctgacct tggcgggggt gctccaggcc
     1621 atggactcca ccctggagga ggctgccctg gacctgaacg ccggacgctg gagggcattc
     1681 cgcaccgtga ccctgcccct ggcggtcccg ggcatcctgt ccgcgtggct cctggtgttc
     1741 acgaactccc tggcggactt cgccaatccc ctcctgctgg cggggaacta ccgggtcctc
     1801 tccgtggagg cctacatcca ggtgaccggc cggaacgacc tgggaggcgg agcggctcta
     1861 tccctcctgc tgctcctgcc cacgttgacg gccttcttcc tccagcggaa ctgggtggcc
     1921 aagaggtcct tcgtgaccgt cacgggcaag cccaacgcca agctggtgga gatcgcctcc
     1981 ccccgggtgc gattcgccct gggggccggg gtgtggacct gcgtcgccct catcctgggc
     2041 ctctacggca caatcgtggc gggctgcttc gtgaagaact gggggatcga ctactccttc
     2101 accctgtcca acattggcga ggccctgacc cggggccggg acgccatccg gtccaccgtg
     2161 accctggccg cagcggccac ccccgtggcg ggccttctgg ccatggcggc ggccctggtc
     2221 ttcgtgcgca agaccttcgc gggcaagcgg gtcctggaag ccctgatgat gacccccttc
     2281 gccgtgccgg ggactctgtt gggcatcgcc tacatcctgg ccttcaacaa ggcccccctg
     2341 atcctggtcg gcacctcggc catcatcgtg atcaactacg tcatacggga gcttccggtg
     2401 gggatcgagt cgggcgtggc ctccctgcgg cagatcgacc cctccatcga ggaggcggcc
     2461 aaggacctgg gggccgacga gggcaccgtc ttccggacca tcgtcctgcc cctggtgcgc
     2521 ccggccttca tcaccagcat gtcctacacc ttcgtgcggt ccatgaccgc ggtcagcgcg
     2581 gtgatcttcc tgatctccgc ccgctggtac cacatgacgg tgctgatcta caacttctcg
     2641 gaaaacctcc ggttcggact ggccagcgtg ctggccacgg tgctcatcgt catcgtcctg
     2701 ggcgctttcg ccctgatgcg gctcctggtg cgggacacgg gagccttgga aaagaccatc
     2761 acccggtagg ggaggaaagg ataccacatg gccaagcaac cggtagcggt gaccctggac
     2821 gacgtgaccc gggtgtacaa gaacgtgaaa gggcggcccg acgtggtcgc cgtgcgccag
     2881 gcctccttcg aggtggcccc cggggagctc gtgaccctcc tgggaccctc gggctgcggc
     2941 aagaccacca ccctgcgaat gatcgccggc ttcgacctgc ccagctccgg cctcatacgg
     3001 atcggcggtg aggacgtgac gaaccttccc ccgaacaagc gggacaccgc caccgtgttc
     3061 cagagctacg gcctcttccc ccacatgacc gtggcggaga acgtggccta cggcctgcgg
     3121 gtccggaacg tgcccaaggc cgagcaggag gagcgggtcc gccgagctct gtccctgacg
     3181 gggctttcgg actacgccga acgctccccg ggccgcctgt ccggcggcca gcagcagcgg
     3241 gttgccctat cccggagcct cgtggtggag cccggggtcc tcctgctgga cgagccgctt
     3301 tcgaacctgg acgccctcct gcgggagcag atgcgggtgg agatccgccg catccagaag
     3361 tccctgggca tcaccgccgt gtacgtgacc cacgaccgga ccgaggccat gagcctttcg
     3421 gatcgggtta tcgtcatgaa ggatggccgg atccgccaga tcggctcgcc ctcgacgatc
     3481 tacgaggacc cggactccgt gttcgtggcg ggcttcgtgg gcaaggtggc cttcttcccc
     3541 gtggccgtgg agggcctcga agggcccggt tgcgcctgtg ttctccacgg gaagaccgtg
     3601 cgagccggga aacgcgcccc cggggtggcc gccggcaaac ccggcatcct catggcccgc
     3661 cccgagtccc tgcgtctggg agcccccggc cagggcgtgg cggacgggac cgtccggcag
     3721 aacgtgtacc tgggctccag cgtggagacc ttcgtgacca ccgagtacgg ggagttgttg
     3781 gtgcaggtgg acgaccccgc gggcaagcgc atccccgcag agggggaggc cgtgtccatc
     3841 tcctttaacg aggcccgggt aagggtgctg ccgccggaga cggaataaat tcatggggac
     3901 agagctcgat gcaatcatat cgagctctgt cgcgcgtatt gcaggcatga gaaagccaag
     3961 agagttgcgc gaaggcgccc gctaccatgt gacgggccgg gcgaatcgga aagaactaat
     4021 cttcgacaca ggagcgatga aggatctttt tctttcggtc gtgaaacggg ctaaaaggaa
     4081 atatgccttt cggatcgaga atttttgcat tatgggaaat catttccatc ttgtgatcat
     4141 tccggggaag ggcatgagct tatccacgat catgcagtgg atcatgagcg tctttgctat
     4201 ggcgtacaat cgtatctgcg gttatacggg tcatgtgtgg ggggaacggt tcttctcccg
     4261 gatactatcg gggttccggg aattgctcgc ggtattcgca tacatcgaca ataatccggt
     4321 acaagcgagc ctgatagagg atccccgcaa gtggagattc gggggattat ggcataaccg
     4381 tacagggtgc cgtgatatcg tcgaagtaga accatcctgg ttgcctctta cctttccgga
     4441 acggggaatg ctcctcctga cgaactgaaa cgcccagaaa agcctgcctg tcggagtgta
     4501 ccgcgatccc cgggcacggg gattccggat gggcagcagg gtaccgtggt atcggaggcg
     4561 ggctcccggg gtggagcccg ggtcctgccc ccgaatacgg actagcgcgg ggcgggagtc
     4621 attcaggact tcccgagagt ccctgcgacg ctcgactatg tctccgagac atttcggaga
     4681 ctcccgaaga cctatccacc tgtccatgga ggattccacc cgcgtcggga agctcggcgg
     4741 ccgggcgacg aacggcggcc gcggatgccg tatccctgag tatatttgtt cacggaggta
     4801 cctccatgaa cgacgccatc ctgagcgccc cgcccgcggg aaacgaaccc gtccgttcct
     4861 acgcccccgg cacgcccgag cgggcggccc tcaaggcgga gctcgggtcc ctgctgtccg
     4921 agagcttcga catacccttg atcatcggcg gccgggagct gcggacggga cgcacggccc
     4981 ccctcgcgca gccccacgac cacggccgac cgctggggcg gttccacctg gccggggaag
     5041 aggaggcggc cctggccgtc gacgcggccc tcgcggccaa gcccggttgg gaggccatgc
     5101 cctgggagga gcgggcggcc gtcttccgcc gcgccgcggc cctggtcgcc ggcaagtacc
     5161 gctaccgcct cctggccgcg accatgctcg gccagtctaa gaccgtccac caggccgaga
     5221 tcgacgcggt ctgcgaaacc gcggatttcc tccggatcaa tccccgcttc atggaggagc
     5281 tctactccat ccagccgcga agctccgatt cggagtggaa ccgcgtccgc taccgccccc
     5341 tggagggctt cgtctacgcc gtctcgccct tcaacttcac ctcgatcgcc gccaacctcg
     5401 cgagcgctcc cgccatgatg ggcaacgtcg tggtgtggaa gccggcttcc acctccgtcc
     5461 tctcgaactg gatcctggcg cggatctacg aggaggccgg cctcccgccc ggggtcatca
     5521 acttccttcc cgggagcggc gccgcggtct cggcggtcgc tctttcccat ccggccttcg
     5581 ccggcctcca cttcacgggc tcgacggcgg tcttcaacgg gctctggagg caggcggccg
     5641 ccaacctggg ttcctaccgc tcctatcccc gcctcgtggg cgagacgggg ggcaaggatt
     5701 tcgtcttcct cgatccctcg gccgacccgg acgccgccgt cgctgccctt gtccggggcg
     5761 cctacgagta ccagggccag aagtgctccg cggcctcgcg gctctacgtg cccgcctccc
     5821 tggggagggc cttcctcgac cgcctggccg agaccgtgcg ctccatcccc gtcggccccc
     5881 cgacggacct ccgcgccttt atgtgcgccg tcatcgacgg ggcctcctac cggaacatcc
     5941 tgccctacct ggagcgagcc tgctcccggg gcgcggcgcg ctgcgagctc gagctcctcg
     6001 cggggggaca cgggagggcc gaggaaggct ggttcatcga gcccagcctt ctcctggccg
     6061 agtccccgga ctacgagacc atgagggagg agctcttcgg gccggtcctg accgcctacg
     6121 tctacgacga ccgggacatg gaggcggcct accgcctcgt ggacgggacc tcgccctacg
     6181 ccctgaccgg cgccgtcttc gcccgggacc ggcgggccat tctggccggc ctggagggcc
     6241 tgaagaacgc tgcgggaaac ctctacgtca acgacaaacc caccggcgcg atcgtcggca
     6301 gccagccctt cggcggcatg cgggcctcgg gcaccaacga caaggccggc tccatcctca
     6361 acctgatccg ctggacgagc gccggggcgg tcaaggagaa cttcgatccg cccgtcgact
     6421 ggcgctaccc ctacatgtcc gaagagtagg agcgcgtcgc ggcctcgcgg cgacgaagtg
     6481 cttcgcgctt cgtcgagtcc atgcgattac ggtacgaggg ggtagcggag aagcgccgcg
     6541 cggtacgcgc ggccttcgga gcgaggggga gccgcgcctg aggaccccgg ctccggcgcg
     6601 gcataaaacc gcgaccgcag cctatctctg ctattgcgcc ggagtcgggc gcacccgcgg
     6661 aagcatgcgg gaagcgccgc ttcactccca gcaacgcgcg ggtgcggagg gaacccacgg
     6721 ctcgcggttc cgcaggcgaa gccgaggacg tagcgagccg tgggttcccg cagcaggccc
     6781 cgcgttgatc gggataccgg cgcttcccgc ttttcgtcgc gggcgctccc cctacttctc
     6841 cgtgtccgtc atccccgcga tgaactgctt ctgcatgacc actacgacca gggccggcgg
     6901 gatcagggcc agcatggtgg tggccatgac gatctgccac tcgatgagga cgtccgcgcc
     6961 ctggagcatg cggttgatgc cgatgaggag ggtgtagtac tcctgcttgg tcgtgatgag
     7021 gaggggccag aggtactggt tccagccgta gatgaactgg atgacgaaca tggccgcgat
     7081 gggggtccgg gtcatgggca ccaggatgcg gcggaagaac tgcatggcgt gggccccgtc
     7141 gatctgggag gcctcggcga tctcacgggg gaccgagagg aagaactgac ggaacaggaa
     7201 caccgcggtg gcggacacga ccatgggaag gatgagcccg gcataggtgt tcaagaggcc
     7261 caggtcggag atgaccttgt aggtgggcat gatgcggact tccaccggga gcatgagggt
     7321 gatgaagatg ctccagaagc agaaggcgcg gagcttgaag tcgaagaaga cgatggcgta
     7381 ggccgccagg agggacacga cgatctttcc gaccgcgatg cccatggcca tgatgaagga
     7441 gttcttcagc atggtgaggg ccgaggcgcc catgttcgcg gagccctcca ggatggcccg
     7501 gcggtaattt tccaggaagt ggggacccgg gctgatgggc atgggggccg agaggatctc
     7561 cgcgttcgtg cgggtggagg cggtgaagac gagccagatg gggaagagga tcaccatcac
     7621 ggccaggatc aggaagacat ggggcaggac gcggcggaag cgggagtgtt cggtcatcgc
     7681 ggtgccccct tagtagttga cgcgccgctc gatgaagcgg aactgcacga tggtgaggcc
     7741 gatgaccatg agcatcagga tcaccgactg ggcggaggaa cctcccaggt ccaggttcac
     7801 gaccccgtcc ctccagacct tgtacaccag gatggccgtg gacttgcccg ggcctcccgc
     7861 ggtgacctgg tggacgatgg ggaaggtctc gaagaagccg tacacgaggt tcatcaccag
     7921 gaggtagaag ctggtgggcg agaggatcgg gaagatgatc ttccagaagc ggcgggcc
//
LOCUS       DAOTEI010000161         7950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6442_length_7950_cov_5.564408, whole genome shotgun sequence.
ACCESSION   DAOTEI010000161 DAOTEI010000000
VERSION     DAOTEI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_7950_cov_5.564408"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="P5313_13385"
     CDS             <1..503
                     /locus_tag="P5313_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81407.1"
                     /translation="RQYERDFEGLMSNPIRAAAYAKAAREDNLYNVTIDGLKVTLGKK
                     ASQTVHYGHMDKANKASVYMPLNAGSIGYRYNNEFLLDFFKANPTTDFAAFTMKKAKV
                     TLAEDKNVDAGAKAAEYTAADDMVFAYADVVTGATYSDFPHYALELQTAYQMALAMQH
                     KAMKKK"
     gene            1070..1405
                     /locus_tag="P5313_13390"
     CDS             1070..1405
                     /locus_tag="P5313_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HRY81408.1"
                     /translation="MVHESIESFVCRNCGSPVPPSSIGGKNRNHCHVCLSSRHVDITT
                     GDRRSPCKGLMRPIAVWVKPDGEWSILHRCERCGVIRANRIAADDDERALLKLALKPV
                     TRLPFPLEV"
     gene            1410..2621
                     /gene="rsgA"
                     /locus_tag="P5313_13395"
     CDS             1410..2621
                     /gene="rsgA"
                     /locus_tag="P5313_13395"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HRY81409.1"
                     /translation="MNRVSDAESWGWKPEFHASWAAAMEQQGEGKRILPARVTARGHH
                     RYEVIIPDLRGRPGFPDCPGLAGIHENVAVSGRFRYDAEDPEDYPVVGDWVLVDPDED
                     SIRIRSVLHRRSALTRGRAGRESGGQVLAANIDTLLLVFALDGGRNFLPRLLERSLVV
                     ARNSGAAPCIILNKADLASEEDRERAFLEAAYAAPSVPVLAVSAKTGEGLEDLRARLS
                     PGETIGMLGKSGVGKSALVNALARRGRDEPGHDEGLRKTGISSPASDESAREGRVRPG
                     DLRGRHTTTTSRLYRLASGLLLIDGPGIRELKIWGGVEGLEEGFPELAALAGRCRFKD
                     CSHSGEPGCAVLEALDSGELEPSRYLSYLELSRERDRLEKRTDERARREDERKWKQIS
                     KQRKALKQSGW"
     gene            complement(2676..3560)
                     /gene="dapA"
                     /locus_tag="P5313_13400"
     CDS             complement(2676..3560)
                     /gene="dapA"
                     /locus_tag="P5313_13400"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRY81410.1"
                     /translation="MSLHGVYVVTVTPFTRDGKVDLAGIEKNADWLVKQGVHGLLPLG
                     STGEFASLEEEDKKAIVDRVLKTVAGRVPVVVGATAETTDKAIRNARYAESAGAAGVL
                     VLPPYYYTPDQEEIYDHYRRIAEAVKIPVMIYNNPASSKVDIRADTVARLARVPGIEC
                     IKESTGDIRRITEIRLLTEDKLTVFCGWEDMAYESFVMGARGWVCVLGNILPKECVEL
                     HDLIVVKKDLPAAWTLYRKLLPLLRYLEYAGKTQKALKYVLDKTGLAGGASSSPKRPL
                     DPEDKAALDRMLADLGKA"
     gene            complement(3598..5475)
                     /locus_tag="P5313_13405"
     CDS             complement(3598..5475)
                     /locus_tag="P5313_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRY81411.1"
                     /translation="MSDTLGGYAGKCLHLDLSVPSGRTEDLDPEILRDYLGGTGYAAR
                     ILYDGQKPGIDPLSPEAMLVVSTGPLTLNAVPGGGSVELCFKSPLTGGWGESRAGSDF
                     GPDLRRAGFDHVVIRGRAPRPVYVVIRDGKAEFRPAEHLAGKLVSEKLAMIRRELPDG
                     RFSIGCIGPGGENEVAFAGVQFGDRAAGRAGAGAVLGSKNLLAIAVEGRNEVVAAKPA
                     ELKELLRTYFTQLRSNPVSIGFHDNGTVGDLAANDEKGDWPTKNWQSNSWGKGAVLLD
                     SFVRRNFIKPYPCYRGCTISCGRKVRVPDGAYATVEHGGAEYESISCFTAYVQNEDMD
                     AAVHCSHLCNEYGVDTISAGAVIAFALECAEAGSLAASDIAGLDLSWGNSKVLPRLVR
                     MIALREGIGAVLADGVRAAAARIGKGSEAFAVHVKGLEGPAHDPRSGKSLAVSYGTGN
                     RGMCHIHPAEAMAWDSGKMDWGLCKHGLPDPNGVDRWDEPGKGSAAKLIQDGLNLPDI
                     LGTCKFFMYAGIGVDELAAMLSLITGRDIGPAELLAVSERVINLQRLFNLREGFTAAD
                     DLLPARVCAVPSFGRYAGTAECGISDFPGMLREYYEARGWDAGTGIPTDATLKRIGLR
                     R"
     gene            complement(5516..5794)
                     /locus_tag="P5313_13410"
     CDS             complement(5516..5794)
                     /locus_tag="P5313_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81412.1"
                     /translation="MRVRLAAMGQLEKDFGGGRFDFEFPPDSTLADLLVKIGDDFSGR
                     LPESLWNRAERKFRGAVVMMSDGAALRDPAQPLKDGQEILIFKVLVGG"
     gene            complement(5796..6734)
                     /locus_tag="P5313_13415"
     CDS             complement(5796..6734)
                     /locus_tag="P5313_13415"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY81413.1"
                     /translation="MSKAEKDYAVTPGSLTAYISRDLENRILSGDLEDGDLSLVTLAA
                     HYNVSITPLRAAVQELVDKGVMIKLPNRRLRANPGKARKAEPRRVVAVPPTQRDWSEV
                     LIKEVVRASLSPDPVYLREDTLAKKFGVGRSIIRQVLSRFVGAGLIDHIPRKGWMAHP
                     LGVEDMNAYLTVREILELKALKLARPRLDMEELARIREVCKSGARGNLKDFDNSVHEC
                     IINASGNRYIRQFFQQHVARYYTELFYFAAPETAVVEEMSNEHLGIIDALLERRWPAA
                     EKLLSAHIFGQAVVLTKLLEKERGEAPGRNDARKRF"
     gene            complement(6773..>7950)
                     /locus_tag="P5313_13420"
     CDS             complement(6773..>7950)
                     /locus_tag="P5313_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRY81414.1"
                     /translation="GGMVKMEKFNGFEVAICGDFHELLITVDSGGPSLEGVLERIKEA
                     IPRYEDIAVCERSPRTLVNMKRSADIDEGTLQALRRIPMVLDVVSLAPILPTRSSADC
                     RVPFVTAEQVLEWMAANGRKELWELAALYEARRGDTDQDDVMEKMSRLVDIMRKSIRD
                     GLAGTEYADRILGPQAYKIDEGIKNNRLIPGDILNRIIKYITAVMETKSSMGVIIAAP
                     TAGSCGCLPGTILGAGDAMDFSREEITKAMLAAGLIGVFVSEGATFSAEVAGCQVECG
                     AGSAMAAAGLVQLFHGSAEQCLDAASMAFQNVTGLACDPVANRVEVPCLGKNIMGGSN
                     AVACANMALAGYDKVIPLDETIAAVYDIGTKLPLELRCTFGGLGKTRTSLDIQKRLT"
BASE COUNT         1345 a         2715 c         2481 g         1409 t
ORIGIN      
        1 cccgccagta cgagcgcgac ttcgagggcc tcatgtccaa ccccatccgc gccgccgcct
       61 acgccaaggc cgcccgggag gacaacctct acaacgtcac catcgacggg ctcaaggtca
      121 ccctcggcaa gaaggcctcc cagaccgtcc actacggcca catggacaag gccaacaagg
      181 cctccgtgta catgcctctg aacgccggat ccatcgggta ccgctacaac aacgagttcc
      241 tcctggactt cttcaaggcc aatcccacca cggacttcgc cgcgttcacc atgaagaagg
      301 ccaaggtcac gctcgccgag gacaagaacg tcgacgccgg cgccaaggcc gccgagtaca
      361 ccgccgcgga cgacatggtc ttcgcctacg cggacgtcgt gacgggagcc acctactccg
      421 acttcccgca ctacgccctg gagctccaga ccgcctacca gatggccctg gccatgcagc
      481 acaaggcgat gaagaagaag tagtctacat cctgtaggcg ctgaaggcgc ttcgcgcctt
      541 cagcgatttc ctgtgccgca gcatacggcc ctgcgggccg tttgccgaga ggaaaagata
      601 gtcgtccgcg ctatcgcgca ggacggcatc cgtgccgcgc aagaaaaggc cggggtcccg
      661 acggggcccc ggtttttttt gtcggggaac cggcgatacg acgaaaatcg tagcctcccg
      721 agcggcccgt ccgattgacc taaactcgcc gcgcgggtat ccttgtttca acctggcatt
      781 cgaggaggtc tgtacgaacg ctcttgtgat ccatccgacg gcggtgttcc ggcaggaatg
      841 acgcgggccg gccaagcgcc cccgtggttt ctggttcgtc agatccctcc accctccttt
      901 gagggcggtt cccgacgagc cctcctgccg tacggactct tccgcacccc tccgtccgcc
      961 gaacaccccg tccatcatcc cacgcggtac tcattcacac cggccccggg aacctcctta
     1021 tattccagcc ccggtgccga aaggcgttgg tatgtccagg acacaacacg tggtacacga
     1081 gagcatagaa tccttcgtct gccgaaactg cggcagcccg gttcccccat cctcgatcgg
     1141 gggcaagaac cgcaatcact gccatgtctg cctttccagc cggcatgtcg acataacgac
     1201 cggtgaccgc cgttcaccct gcaagggcct catgcggccc atcgccgtgt gggtcaagcc
     1261 cgacggagaa tggagcatac tgcaccgctg cgagcgctgt ggagtaatcc gagccaaccg
     1321 catcgccgcg gatgacgacg agagagccct gctcaagctt gccctcaagc ccgtaacgag
     1381 gcttcccttc cctctggagg tgtgacacga tgaaccgcgt ctcggacgcc gaatcctggg
     1441 gctggaaacc cgaattccac gcttcctggg ctgcggcgat ggaacagcag ggcgagggca
     1501 agcggatcct tcccgcccgg gtgacggctc ggggccacca ccggtatgag gtgatcattc
     1561 cggaccttcg cggacgcccg ggattcccgg actgcccggg tctcgccgga atacatgaga
     1621 acgtggccgt cagcggccgc ttccggtacg atgcggagga tcccgaggac tatcccgtgg
     1681 tcggagactg ggtcctggtg gatccggacg aggacagtat ccgtatccga tcggttctac
     1741 accgccgctc ggccttgacg cgaggccggg cggggaggga gagcggcggg caggtcctgg
     1801 cggccaacat cgacacgctc ctcctggtgt tcgccctgga cgggggacga aatttcctgc
     1861 cgaggctcct cgagaggagc ctcgtggtgg cgcgcaattc cggcgccgcc ccctgcatca
     1921 tcctcaataa agcggatctg gcatcggagg aggaccggga acgggctttc ctggaggccg
     1981 cgtatgccgc tccctcggtt ccggtcctgg ccgtaagcgc caagaccggg gaaggccttg
     2041 aagatctgag agcccgccta tctcccggag agacgatcgg aatgctcggg aaaagcggag
     2101 tcgggaaatc ggccctggtg aacgcccttg cccgccgcgg ccgggacgag cccgggcacg
     2161 acgaaggttt acggaagacc ggaatctcct ctccggcctc ggacgagtcc gcccgggaag
     2221 gtcgagtccg ccccggcgat ctccgcggaa ggcacaccac gaccacaagc cgcctgtatc
     2281 ggctcgcatc cggcctgctc ctgatcgacg gtcccgggat ccgggagctc aagatctggg
     2341 gaggcgtcga gggcttggaa gaaggctttc ccgaactggc ggccctggcg ggacgatgcc
     2401 ggttcaagga ctgcagccac tcgggagagc cgggctgcgc ggtcctggag gctctcgact
     2461 cgggagagct cgaaccctcc cggtacctgt cctacctgga actgtcccgg gagagagacc
     2521 ggctggagaa gaggaccgac gaacgagccc gtcgggagga cgagcggaag tggaagcaga
     2581 tatcgaaaca gcggaaggcc ctgaaacaat cgggatggta agggggagtc tccgccgagg
     2641 acgccggatc cgcggcggag accttcgtcg gtgctctacg ccttccccag gtccgcgagc
     2701 atcctgtcga gggcggcctt gtcctccgga tccagagggc gcttggggct ggaggaggcg
     2761 cccccggcca ggcccgtctt gtccagcacg tacttgaggg ccttctgggt cttgcccgcg
     2821 tactccaggt agcggagcag ggggagcagc ttccggtaca gggtccaggc cgccggaagg
     2881 tccttcttca cgacgatcag gtcgtggagc tcgacgcatt ccttgggcag gatgttgccc
     2941 aggacgcaga cccagccccg ggcccccatc acgaaggatt cgtaggccat gtcctcccag
     3001 ccgcagaaga ccgtcagctt gtcctcggtc aggagccgta tttcggtgat ccggcggatg
     3061 tccccggtgc tctccttgat gcactcgatc ccgggcaccc gggcaagccg ggccaccgtg
     3121 tcggcccgga tgtcgacctt cgagctggcc ggattgttgt agatcatcac ggggatcttg
     3181 acggcttccg cgatgcgtcg gtaatggtcg tagatctctt cctggtcagg ggtgtagtag
     3241 tacgggggga ggacgagcac ccccgcggcg cccgcgcttt cggcgtaacg cgcgttccgg
     3301 atcgccttgt cggtcgtttc cgccgtcgct cccaccacca cgggaaccct gccggcgacg
     3361 gtcttcagga cccggtcgac gatggccttc ttgtcctcct cctccaggct ggcgaattcc
     3421 ccggtgctcc ccagggggag caggccgtgg actccctgct tcaccagcca gtcggcgttc
     3481 ttctcgatcc ccgcgagatc caccttcccg tcccgcgtaa agggagtgac ggtcactacg
     3541 tacacaccgt gcagactcat tccatcctcc atcccggtta ttattctcgt ctggtcatca
     3601 tcggcgcagg ccgatccgtt tcagcgtcgc gtccgtggga atccccgtac cggcgtccca
     3661 gccccgggcc tcgtagtact ccctcagcat tccggggaaa tccgagatgc cgcactccgc
     3721 cgtccccgcg taccttccga aggagggcac ggcgcagacc cgcgcgggca ggaggtcgtc
     3781 ggcggcggtg aatccctccc gcaggttgaa caggcgctgc aggttgatca cccgctcgga
     3841 aaccgccaga agctcggccg gcccgatgtc ccgtcccgtt atcagggaga gcatcgcggc
     3901 cagttcgtcc actccgatgc ccgcgtacat gaagaacttg caagtaccca ggatgtcggg
     3961 caggttgagg ccgtcctgga tgagcttggc cgcggaaccc ttcccgggct cgtcccagcg
     4021 gtccaccccg ttcgggtccg gcaggccgtg tttgcacagg ccccaatcca tcttgcccga
     4081 gtcccaggcc atggcctccg cggggtggat gtggcacatc ccccggttcc cggtcccgta
     4141 gctgacggcc agggacttgc ccgaccgggg atcgtgggcc gggccttcca gccctttcac
     4201 gtgcacggcg aaggcttcgg aacccttgcc gatcctcgcg gccgcggccc gaaccccgtc
     4261 ggccagcacg gcgccgatcc cctcccggag ggcgatcatc cggaccagcc ggggcagtac
     4321 cttcgagttg ccccaggaga ggtccagccc ggcgatgtcc gaggccgcga gacttcccgc
     4381 ttccgcgcat tcgagcgcga aggctatgac cgccccggcg gagatcgtgt ccacgccgta
     4441 ttcgttgcac aggtgggaac aatggacggc ggcgtccatg tcctcgttct ggacgtaggc
     4501 ggtgaagcag gaaatggact cgtactcggc tcccccgtgc tcgaccgtcg cgtaggctcc
     4561 gtcgggaacg cggaccttcc gcccgcagga aatcgtgcaa ccccggtagc agggataggg
     4621 cttgatgaag ttgcgccgga cgaagctgtc cagcaggacg gcgcccttgc cccaggaatt
     4681 ggactgccag ttcttcgtgg gccagtcccc cttctcgtcg ttcgccgcga ggtctccgac
     4741 ggtcccgttg tcgtggaacc cgatggagac cgggttcgag cgcagttgcg tgaagtacgt
     4801 gcggaggagc tctttcagtt ccgcgggctt cgcggcgacg acctcgttcc ggccttccac
     4861 cgcgatcgcc aggaggttct tcgatccgag gaccgccccg gcccccgccc tgcccgccgc
     4921 ccgatccccg aactggaccc ccgcgaaggc gacttcgttc tcgccgccgg ggccgatgca
     4981 gccgatggaa aacctcccgt ccggaagctc ccttcggatc atcgcgagtt tttccgagac
     5041 cagcttcccg gccagatgct cggcgggccg gaactcggcc ttcccgtccc gtatcaccac
     5101 gtagacgggc cgcggggccc tgccccggat gacgacgtgg tcgaacccgg cccggcgcag
     5161 gtccggcccg aagtcgctgc cggcgcggga ctcgccccaa ccgcccgtga ggggagactt
     5221 gaagcagagt tccacgctgc ctccgccggg aaccgcgttc aacgtcaggg ggccggtcga
     5281 gacgaccagc atcgcctcgg gcgaaagcgg atcgatcccg ggcttctgcc cgtcatagag
     5341 gatcctggcc gcgtatcccg taccacccag gtaatcccgc aggatttccg ggtccaggtc
     5401 ctcggtccgg cccgacggga cgctcaaatc caggtgaagg catttcccgg catatccgcc
     5461 aagtgtatcg gacatcgctc atctccttcc cccggaggcg ggggttcgct cggcgtcagc
     5521 cgccgaccag taccttgaag atcaggattt cctgcccgtc cttcagaggt tgggcggggt
     5581 cccgaagggc cgcgccgtcg gacatcatca cgacggcgcc gcggaatttg cgctcggccc
     5641 ggttccacag ggactcgggc agtcggccgg aaaagtcgtc gccgatttta accagcaggt
     5701 cggctagggt ggaatccggc gggaactcga agtcgaacct cccgcccccg aagtcctttt
     5761 ccaattgtcc catcgcggcc aggcggacac gcatttcaga accgcttccg ggcgtcgttc
     5821 cgaccgggcg cctcgccccg ttccttttcc agcagcttcg tcaggacgac ggcctgcccg
     5881 aaaatatgcg ccgaaagaag tttttccgcc gcgggccagc ggcgctccag aagggcgtcg
     5941 atgatgccca ggtgctcgtt cgacatctcc tccaccaccg cggtctcggg ggccgcgaaa
     6001 tagaagagtt ccgtgtaata ccgggcgacg tgctgctgga agaactggcg gatgtaacga
     6061 ttcccggagg cgttgatgat gcactcgtgg acgctgttgt cgaagtcctt gaggttgccc
     6121 ctcgccccgc tcttgcagac ctcccggatt ctcgcgagtt cctccatgtc cagccgcgga
     6181 cgcgccagct tgagggcctt gagttccagt atctcccgca ccgtcaggta ggcgttcatg
     6241 tcctccacac ccaaggggtg ggccatccat cccttgcggg gaatgtggtc gatgagcccc
     6301 gccccgacga agcggctcag gacctgccga atgatggacc tgcctacgcc gaacttcttc
     6361 gcgagcgtgt cttcccgcag gtagacggga tcggggctga ggctggcgcg caccacttcc
     6421 ttgatcagga cctcgctcca gtcccgctgg gtcgggggga cggccacgac ccggcggggc
     6481 tccgccttcc gggccttccc gggattcgcg cgcaggcgcc ggttcggcaa cttgatcatg
     6541 acccccttgt cgaccaactc ctggaccgcc gcccgaaggg gggtgatgct gacgttgtaa
     6601 tgcgcggcga gagtcacgag ggacagatcc ccgtcctcca gatcccccga caggatacgg
     6661 ttttccaggt cccggctgat ataggcggtc aggctgcccg gggtgacggc atagtccttc
     6721 tcggctttgg acatggtcgg ctctccaaat ggtaggctat tcatctgcca tcttatgtaa
     6781 gccgtttttg tatgtccaga gaggtcctgg tcttgcccag ccccccgaag gtgcagcgca
     6841 actccagcgg cagtttcgtg ccgatgtcgt acaccgcggc gatcgtctcg tccaggggta
     6901 tgaccttgtc gtaccccgcc agggccatgt tggcgcaggc cacggcgttg gaacctccca
     6961 tgatgttctt tcccaggcag gggacctcca cccggttggc cacggggtcg caggccagcc
     7021 ccgtgacgtt ctggaaggcc atggaagccg cgtccaggca ttgctccgcg ctgccgtgga
     7081 acaactggac cagaccggcc gccgccatgg cggaacccgc cccgcactcg acctggcagc
     7141 ccgcgacctc cgcggagaac gtggcacctt ccgagacgaa caccccgatc aaccccgccg
     7201 ccagcatggc cttggtgatc tcctcccggg aaaaatccat cgcgtccccg gcccccagga
     7261 tcgttccggg caggcagccg caggatcccg cggtgggggc ggcgatgatg acacccatgg
     7321 aactcttcgt ttccatgacg gccgtgatgt acttgatgat ccggttcagg atgtcgcccg
     7381 gaatgagacg gttgttcttg atgccctcgt cgatcttgta cgcctgaggg cccaggatcc
     7441 tgtcggcgta ctcggttccc gccagtccgt cgcggatgct ctttctcatg atgtcgacga
     7501 gacgggacat cttctccatc acgtcgtcct ggtccgtatc cccgcgccgg gcctcgtaga
     7561 gggccgccag ttcccacaat tccttccggc cgttcgcggc catccattcc aggacctgtt
     7621 ccgcggtcac gaagggcacc cggcagtcgg cgctcgaccg ggtcgggagg atcggagcaa
     7681 gggagacgac atccagcacc atcggaatcc gtctcagggc ctggagcgta ccctcgtcga
     7741 tatcggcgct ccgcttcatg ttgaccaggg tccgggggga ccgctcgcat acggcgatgt
     7801 cctcataccg ggggatcgct tccttgatcc gttccagcac gccttccagg gaaggccctc
     7861 ccgagtcgac ggttatcagc aattcgtgga aatccccgca gattgcgacc tcgaagccgt
     7921 tgaacttttc catcttgacc atgccgccgc
//
LOCUS       DAOTEI010000162         7946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6445_length_7946_cov_8.193131, whole genome shotgun sequence.
ACCESSION   DAOTEI010000162 DAOTEI010000000
VERSION     DAOTEI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6445_length_7946_cov_8.193131"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..365)
                     /locus_tag="P5313_13425"
     CDS             complement(123..365)
                     /locus_tag="P5313_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81415.1"
                     /translation="MKKEAMDSTAIIAILSVFGLAPMTVFGFILLLKRQKHRVEELRY
                     KKEILELELEREAYKIKLLQEENRKYDRLIHGESDN"
     gene            complement(389..832)
                     /locus_tag="P5313_13430"
     CDS             complement(389..832)
                     /locus_tag="P5313_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HRY81416.1"
                     /translation="MGRERYESLEKIVLSDPDVFPTPELLSKVLGEKIDLFHQTLEYL
                     KIEIPEVDPEWNYYRDGKSWILKVLSNKKTFCWIFVYEKGFKTTFYINGKYEEYIIES
                     DLPEDLKKAYLESNGKKIRSLSVNIEKEKDIHDFKKLVKVKKKTK"
     gene            complement(1166..1657)
                     /locus_tag="P5313_13435"
     CDS             complement(1166..1657)
                     /locus_tag="P5313_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3859 domain-containing protein"
                     /protein_id="HRY81417.1"
                     /translation="MKNLVWFFFGVLVLSSCVTTVNQEKVDPVSISILEKGLYTSDII
                     RTEKTPTDVYHVTENHKLLEETALVPLKKGVLFGFKLKLESDKYKELEVEIRLIHPSF
                     YDPYLKKESSSSISKMKIYKEKTRFRGYLLEEDFELAPGKWIYEIWYKGKLYATTEFE
                     LKN"
     gene            complement(2047..3183)
                     /locus_tag="P5313_13440"
     CDS             complement(2047..3183)
                     /locus_tag="P5313_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRY81418.1"
                     /translation="MAIPIEPESRARVFPLADGSAGIEVRPAGAARAELGSIPGSLHV
                     RENLWRIPETFLAFVHSGIPGLRLEFASEGAPVPEAFSLWNGEAVRAETDEPPSSAGL
                     DWNGKVALLSAMRARKYSPRTVRAYYRYVDDFLRRAGKPPRQASDADLTAYLAYLEAE
                     RRASAATLNLAISAVRFFYVAVLGLPLAMRRRRPKGDRRLPMVLSRAEILKVVDALPS
                     LKHKAMILLAYSGGLRVSEIVNLWYQDLDRDRHTIYIRAAKGRKDRYTLLSDTAWTAV
                     ESYVLSERPRFWLFEGRTPGYRLSVRAVQGVFYSACRRAAIGKRVSIHALRHSFATHL
                     LESGTDLRYIQTLLGHASPATTAVYAHVAKGEFLRIRSPLDGAG"
     gene            complement(3294..4349)
                     /locus_tag="P5313_13445"
     CDS             complement(3294..4349)
                     /locus_tag="P5313_13445"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY81419.1"
                     /translation="MPVDTDRADLIARLFPAGVPSLLCPPITHYDAEGALDTARTRAH
                     ISVVAPRAPCLLVPGSTGDGWELSPGEVRRLLEIHAGLARDLGYSILVGALHPDAAEA
                     RRAVLDRLDLFKALAGTANAERALAASRVAGFTVCGPSGADLAQEAIEDSLRRILDLG
                     LPTAVYQLPQVTRNEISPAVMERLLGDYPNLWMMKDTSGSDRLVRAGIRRRGAVFVRG
                     AEGDFASWLADRDGEAERKYDGFLLSSANAFAPHLAEMIRRVRAGDRPAAAELSGRMD
                     SVVRAVFAEAAALPFGNPFSNANKALDHVLAWGGADRAPAPRTRSGSFLPAGLLSAAE
                     VALRRGGFLPGRGYLET"
     gene            complement(4439..5215)
                     /locus_tag="P5313_13450"
     CDS             complement(4439..5215)
                     /locus_tag="P5313_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY81420.1"
                     /translation="MEPDKAFDRIFLQRRSVRAYEDRPIPPEIRDLVLSAALRAPTAG
                     NLCLYTILEIEDPELKSRLSETCDHQSFIARAPWVLVFLADWARLQGYYRHHRVPEWC
                     EERGEPFLRPRESDLMLAACDALIAAQSAASAAEALGLGTCYIGDILENGELHRELLG
                     LPPYAFPITMLCLGWPTDQQRARPQPPRLPRDLVVHRDRYRSYGPADYDRVYAGEGWP
                     EPRTDPPGDNPGRSLYARKFAAAYSLEMRRSVKALLEEWS"
     gene            complement(5302..5841)
                     /locus_tag="P5313_13455"
     CDS             complement(5302..5841)
                     /locus_tag="P5313_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRY81421.1"
                     /translation="MLSKKIVDRVNLQINREIYSAYLYMAMSAKAAEAGYQGVASWLM
                     VQYHEEMFHAMKFYYYLLDQGARPELAKIDAPETKGSTIKEWFTHVLEHEKFVTASIR
                     ELLELALAEKDYASENLLRWFVDEQVEEEKNATEILQQLELLGDGKQGAFLLNIELGK
                     RALTVQSDFSGGIVPTAAG"
     gene            6049..6723
                     /locus_tag="P5313_13460"
     CDS             6049..6723
                     /locus_tag="P5313_13460"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HRY81422.1"
                     /translation="MPNRTPDRYSPGFEPPAGFEARRQRAIDALTQAYASDRITVEEY
                     EARAAAAAAADRPEELEDLVSDLPAPRPSQDAARRGPAPEAPFAPRRPSPPPRPYAPS
                     YPAYGSGEGTVSVACVMGDRRMTGNWLDSDRVNSFTLMGSTRLDLRDADLPESGPVRI
                     EAFTLMGETVIVVPRELPVRLTAVPFMGEAVARRDVDQNVRGARRWVEISGLVVMGSV
                     VVKAAD"
     gene            complement(6727..>7946)
                     /locus_tag="P5313_13465"
     CDS             complement(6727..>7946)
                     /locus_tag="P5313_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRY81423.1"
                     /translation="PASNGLDTLYALQDSEGAIRSAYDISTGKPVLTKENPEGLGLPL
                     FAWAEYNLYHKTGNKKRIKDVMPVLQRHFAWIERTFKRPNGLYAVPVSATGMENSPRG
                     SAVYHADFNAAMAANALYMSAMGDILNDKDIGFQYKRAYFSLKTRINSMMWDAEDSFY
                     YDLDAGERQVKVKTLGGYWPLLAEIPNEDRAERMIAHLLDPGLFGTEHPFPTLAADSA
                     DYDKRGMGFRGSVLPPFTFMVIKGLEKYNHWELARECSIRHLYYILDSMHAAAEKERD
                     HRPIVWEAYQPQNEGKAVWPEKSGWPRQQHLAYAGLSTIALMIENIVGLSISLPRKTV
                     DWIIPNLEIMGIENMSLKRNMVTILSNKSGRGWEIHMESEKLYYFTINVLGKKKKTLP
                     IPSGKCSMLIDKL"
BASE COUNT         1445 a         2408 c         2478 g         1615 t
ORIGIN      
        1 acagggcgcg cgacgcagca gatagcgtaa gttatgtcgc tgatttttct tgagctttct
       61 ccaatatcgc ccgagccagg agggcgaggg cgattacctt ctcccctact cttctctaat
      121 tctcaattat cgctttcacc gtggatcaat cgatcatact tcctgttttc ttcttgaagg
      181 agtttgattt tgtatgcttc tctttcaagt tctaattcta atatttcttt cttgtatcta
      241 agttcttcaa ctctatgctt ttgacgtttc aataaaagga taaatccaaa aacagtcatg
      301 ggggcgagac caaagacact caaaatagca atgatagcag ttgaatccat ggcttccttc
      361 ttcaaaggca aaaggtcttc ctcttgtctt atttagtctt cttcttgacc ttcacaagtt
      421 ttttaaagtc atggatgtct ttttctttct caatatttac tgaaagactt cttatctttt
      481 tcccattact ttccaggtaa gctttcttca aatcttctgg gagatcgctt tcaataatgt
      541 attcttcata tttcccattt atataaaatg tggttttaaa gcctttctca tagacaaaaa
      601 tccaacagaa agtcttcttg tttgaaagga cctttaaaat ccagcttttc ccgtctctat
      661 aataattcca ttcagggtct acttcgggta tttctatctt taagtattcc aaggtttgat
      721 gaaataggtc gattttctct cctaacactt tagataacag ttcaggagtt ggaaacacat
      781 caggatctga tagtacaatt ttttccaaag attcgtacct ttcccttccc aattcttctc
      841 ttagctacgt tgatctcatg ttccaggctc ttacgctttc cgcgccaagg atggcgcgga
      901 aacaccaacc tgtagccgcc aagaaaaatc actgcacata acaacaagct atctggaagg
      961 accgtgcgtc cataagagcg aatcggagta ctcgacgatt cgcgggacgc acggtccttg
     1021 gtggagcggc gcggctagcg ccgcggaacc cgaggagcgc gccacagggc gcgcgacgca
     1081 gcagatagcg taacgttatg tgtctgattt ttcttgaaca tctttcagat cgcccgagcc
     1141 aggagggcga gggcgattac cttccctaat tctttaactc aaattctgta gttgcgtata
     1201 gttttccttt ataccaaatc tcataaatcc atttccccgg agctaattca aaatcttctt
     1261 caagtagata tcctctaaat cttgtctttt ctttataaat cttcatttta gaaatagatg
     1321 acgaagactc ctttttcaaa tagggatcat agaaacttgg atgaatcagt cgtatttcaa
     1381 cttcaagttc tttgtattta tcactttcaa gttttagttt aaatccgaaa agaacacctt
     1441 tttttaacgg aaccagagca gtttcttcaa gcaacttatg attctcagtt acatgataga
     1501 catccgttgg agttttttct gttcgaataa tatcagaagt atagaggcct ttttctaaaa
     1561 ttgaaataga aactggatcg actttttctt gatttacggt tgttacacat gacgaaagga
     1621 ctaatactcc aaaaaagaac cagacaagat tcttcatacg ttttcctcct gtgaattgtt
     1681 tcagaaagta cctttttatt tattttccgc gccacggatg gcgcggaaac acaagccagt
     1741 agccgccaag aaaaatcatg cacataacaa ttattaggtg atgcgcgtgt aatcgcggtg
     1801 caaccctgaa tatgtgaagt tcgtgtaatc gtggtcggtt ttgtcgtata tgtcctggta
     1861 atatctgcaa tggctcgcca ttggaaccgt aaggattcct tactcggcga gtagagttta
     1921 accgatcgct aggacgctcc gccgcaacag gatacgcccc gaatttcacg atcttttaca
     1981 gaattatacg tctccctctt cgatttcgca acttttttcc tagctttcct gattgccgtt
     2041 gtaatattaa ccagccccat ccaggggact ccgtatcctg aggaactccc ccttcgcgac
     2101 atgggcgtac acggccgtgg tagccgggct ggcgtggccc aggagggtct ggatgtaccg
     2161 gaggtccgtg cccgattcca gcaggtgggt ggcgaagctg tgccgcaggg cgtggatgga
     2221 caccctcttt ccgatcgcgg cccttcggca ggccgagtag aagactccct ggactgcccg
     2281 gacggacagg cggtatccgg gggtccttcc ctcgaagagc cagaaccggg gccgttcgga
     2341 taggacgtag gattccacgg ccgtccaggc cgtatcggag agcagggtgt accggtcctt
     2401 ccgccccttg gcggcccgga tgtagatggt atgccggtcc cggtccaggt cctggtacca
     2461 gaggttgacg atctcgctga cccggagccc cccgctgtac gccagaagga tcatggcctt
     2521 gtgcttcagg ctcggaagcg cgtccacgac cttcaggatc tccgccctgc tcaacaccat
     2581 gggcagccgc cggtctccct tcggacggcg gcggcgcatg gcaaggggga gtcccaggac
     2641 cgcgacgtag aagaaccgca cggcgctgat ggccaggttc agggtggcgg cggaagccct
     2701 ccgctcggcc tcgagatagg cgaggtaggc ggtaaggtcg gcgtccgaag cctggcgggg
     2761 tggcttgccc gcccggcgaa ggaaatcgtc cacgtatcgg taataggccc gtacggtccg
     2821 gggagagtac tttcgggcgc gcatggcgga gaggagggcg accttcccgt tccagtccag
     2881 tccggcggag gacggcggct cgtccgtctc ggcgcggacg gcctcgccgt tccagaggga
     2941 aaacgcctcc gggacggggg cgccctcgga ggcgaactcc aggcggagtc cggggatccc
     3001 cgagtgcacg aaggccagga aggtctccgg gatcctccac aggttctccc gcacgtgcag
     3061 ggagccgggg atcgatccca gctcggcccg tgcggctccg gcgggccgga cctcgatccc
     3121 ggcggaaccg tccgccaggg ggaacacccg ggcccgggac tcgggttcga tcggtatggc
     3181 catgcccggt atccgcctgc ggggggcccg gccgcttgcg tcgccgcctc ggacgaggcg
     3241 gcgggacgcc tcggacgagg cggcgggacg cctcgggatt gctcgcccgg ccgtcaggtc
     3301 tccaagtagc cccggcccgg gaggaagccg ccccggcgca gggccacctc cgccgcggac
     3361 aggaggcccg cgggcaggaa ggatcccgag cgggtgcggg gcgcgggcgc ccggtccgcc
     3421 ccgccccagg ccaggacgtg gtccagggcc ttgttcgcgt tggagaaggg gttcccgaag
     3481 ggcagggccg cggcctcggc gaagaccgcc cgcacgaccg agtccatcct gccggagagc
     3541 tccgcggccg ccggccggtc cccggcccgg acccggcgga tcatctcggc caggtggggg
     3601 gcgaaggcgt tggccgagga gaggaggaag ccgtcgtact tccgctccgc ctcgccgtcc
     3661 cggtccgcca gccaggaggc gaagtccccc tccgcgcccc ggacaaacac ggcgccgcgc
     3721 cgccggatcc ccgcgcggac gaggcggtcg gagcccgagg tgtccttcat catccagagg
     3781 ttggggtagt ccccgaggag tcgctccatc acggcggggg agatctcgtt cctcgtgacc
     3841 tggggcagct ggtagaccgc ggtcgggagc cccaggtcca ggattcgccg gagggagtcc
     3901 tcgatggcct cctgggccag gtccgccccg gaaggtccgc agaccgtgaa gcccgccacc
     3961 cgggaggccg ccagggcccg ctcggcgttg gccgtgcccg ccagggcctt gaagaggtcc
     4021 agccggtcca ggacggcccg gcgcgcctcg gcggcgtcgg gatggagggc gcccaccagg
     4081 atcgagtagc ccaggtcgcg ggcgagcccg gcgtggatct ccaggagccg ccgcacctcg
     4141 cccggggaga gctcccaccc gtcccccgtg gatcccggaa ccaggaggca gggggcccgc
     4201 ggcgcgacca ccgaaatgtg ggcccgggtc cgtgcggtgt ccagggcccc ctcggcgtcg
     4261 tagtgggtga tcggagggca caagagcgag ggcaccccgg ccgggaaaag ccgggcgatc
     4321 aggtctgcgc ggtccgtatc cacgggcatg gcggcctcct caggatcggt ctgccctgat
     4381 tctatcacgt cggcggggcg gccggtaccc gccggggacc cgcctccgcc ccgggacttc
     4441 agctccattc ctccaggagc gccttcacgg agcggcgcat ctccaggctg taggcagccg
     4501 cgaatttgcg cgcgtagagg gagcggcccg ggttgtcccc gggcgggtcc gtccggggct
     4561 cgggccagcc ctccccggcg tagacccggt cgtagtccgc gggcccgtag gagcggtagc
     4621 ggtcccggtg gacgaccagg tcccggggaa gccgcggcgg ctggggccgg gctctttgct
     4681 ggtccgtggg ccagcccagg cagagcatcg tgatcgggaa ggcgtaggga ggaaggccca
     4741 ggagttcccg gtggagctcc ccgttctcca ggatgtcccc gatgtagcag gtacctaggc
     4801 ccagggcctc cgcggcggag gcggcggact gggccgcgat cagggcatcg caggccgcca
     4861 gcatcaggtc ggactctcgg ggccggagga agggttctcc ccgctcctca caccactcgg
     4921 gaacccgatg atgtcggtag tacccctgga gccgggccca gtccgccagg aacacgagga
     4981 cccagggggc gcgggcgatg aagctctggt ggtcgcaggt ctccgagagc cgggacttga
     5041 gctcggggtc ctcgatctcc aggatcgtgt agaggcacag attgccggcg gtcggagcgc
     5101 gaagggccgc ggacaacacg agatcccgga tctccggggg gatcggacgg tcctcgtagg
     5161 cccggacgga gcgtcgttgg agaaagatcc tgtcgaaggc tttgtcgggt tccattcccc
     5221 aaccatcgcc cgggtccgga taaaatgcaa gcgccccgcg aaggcgcggg gcgctacggg
     5281 gtagctctcg gcgggggaac ctcagcccgc cgcggtcggg acgatgcccc cggagaagtc
     5341 gctctggacg gtgagggctc gcttccccag ctcgatgttg agcaggaagg ccccctgctt
     5401 gccgtctccg aggagttcca gttgctggag gatctcggtg gcgttcttct cctcctccac
     5461 ctgctcgtcg acgaaccagc gcagtaggtt ctcagaggcg tagtccttct ccgccagggc
     5521 cagctccagg agctcccgga tggaggcggt cacgaacttc tcgtgctcca ggacatgggt
     5581 gaaccactcc ttgatcgtgg aacccttggt ctcgggggcg tcgatcttgg ccagctcggg
     5641 ccgggcgccc tggtccagga gatagtagta gaacttcatg gcgtggaaca tctcctcgtg
     5701 gtactggacc atgagccagg aggccacccc ctggtagccc gcctcggcgg ccttggcgga
     5761 catggccata tagaggtagg ccgaatagat ctcccggttg atctgaaggt tgacgcggtc
     5821 gacgatcttc ttggaaagca tgtatgtctc cttgattttc aggatatgtt cctgtttgaa
     5881 aaagatactg gtcccggagc ccggattcaa gccggaccgg cccgtttttc ccggcttcct
     5941 agttggagcg ggcccccaaa atgaagtaca ttatacacgg cgcagcgcgg gaaaccggcg
     6001 cgcccgggag ccgaaccata ccaaacggtc ccgacaagga ggcccttcgt gccgaacaga
     6061 accccggatc gatactcccc cggtttcgag ccccccgccg gcttcgaggc gaggaggcag
     6121 cgggcgatcg acgccctgac ccaggcctac gcgtccgacc ggatcaccgt ggaggaatac
     6181 gaggcccgcg ccgcggccgc cgcggccgcc gaccgccccg aagagctgga ggacctggtc
     6241 tcggacctcc cggcgccacg cccttcgcag gacgccgccc gccgcggccc cgccccggaa
     6301 gcgcccttcg ccccccgccg cccctccccc cctccccgcc cctatgcccc ctcctacccg
     6361 gcctacggat cgggggaagg caccgtctcc gtagcctgcg tgatggggga ccggcgcatg
     6421 accgggaact ggctggactc ggaccgggtg aactccttca ccctgatggg cagcactcgg
     6481 ctggacctcc gggacgcgga cctgcccgaa agcgggccgg tccgcatcga ggccttcacc
     6541 ctcatggggg agaccgtcat cgtggtgccc cgggagcttc ccgtgcgcct caccgccgta
     6601 cccttcatgg gggaggccgt ggctcgccgg gacgtggacc agaacgtccg cggagcccgg
     6661 cgctgggtgg agatctcggg actcgtggtg atgggctcgg tggtggtgaa ggccgcggac
     6721 tgatcgtcag agtttatcga tcagcatgga gcacttgccc gagggtatgg gcagggtctt
     6781 cttcttcttt cccaggacgt tgatcgtgaa gtagtagagc ttctcgctct ccatgtggat
     6841 ctcccagccc cggccgctct tgttggacag gatggtgacc atgttccgct tcagggacat
     6901 gttctcgatc cccatgatct ccaggttcgg gatgatccag tccacggtct tccggggcag
     6961 ggagatggaa agccccacga tgttctcgat catgagggcg atggtggaga gccccgcgta
     7021 ggccaggtgc tgctgccggg gccatcccga tttctccggc cagacggcct tgccctcgtt
     7081 ctggggctgg taggcctccc agacgatggg ccggtggtcc ctctccttct ccgcggcggc
     7141 gtgcatggag tccaggatgt agtagaggtg ccggatggag cattcccggg ccagctccca
     7201 gtggttgtat ttctccagcc ccttgatgac catgaaggtg aagggcggga ggacggaacc
     7261 ccggaagccc atgccgcgct tgtcgtagtc cgcactgtcc gcggccaggg tcgggaaggg
     7321 atgctcggtg ccgaagagtc ccgggtccag gagatgggct atcatgcgct ccgcccggtc
     7381 ctcgttggga atctccgcca ggaggggcca gtacccgccc agggtcttca ccttgacctg
     7441 ccgttccccc gcgtccaggt cgtagtagaa ggagtcctcg gcgtcccaca tcatggagtt
     7501 gatccgggtc ttcagggaga agtaggcgcg cttgtactgg aatccgatgt ccttgtcgtt
     7561 gaggatatcc cccatggcgc tcatgtacag ggcgttggcc gccatggccg cgttgaagtc
     7621 cgcgtggtac accgcggaac cccgcgggga gttctccatc cccgtggcgg acaccggcac
     7681 ggcgtacagg ccgttgggcc gcttgaaggt ccgctcgatc caggcgaaat gcctctgcag
     7741 gacgggcatg acgtccttga tgcgcttctt gttgcccgtc ttgtggtaga ggttgtactc
     7801 cgcccaggcg aagaggggca ggcccaggcc ctcgggattc tccttggtga gcacgggctt
     7861 tccggtggat atgtcgtagg ccgagcggat ggccccctcg gaatcctgca gggcgtagag
     7921 ggtgtccagg ccgttggagg ccggga
//
LOCUS       DAOTEI010000163         7873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6524_length_7873_cov_5.429138, whole genome shotgun sequence.
ACCESSION   DAOTEI010000163 DAOTEI010000000
VERSION     DAOTEI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_7873_cov_5.429138"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="P5313_13470"
     CDS             complement(<1..686)
                     /locus_tag="P5313_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY81424.1"
                     /translation="MNPDPAIRVFERILADPDIAPCVARVERLPEAPGSFAPLPPDLD
                     GRLAGALARRGIERLYTHQRASWDLARAGRNFVVVTPTASGKTLCYNLPVVQALLEDP
                     SARALYLFPTKALSQDQQAELNEVALGGDLPVKIATYDGDTPSSVRVSARDTGRIVIT
                     NPDMLHSGVLPNHAKWSRFFSGLRYVVVDEMHGYRGIFGSHVANVIRRLKRIAAFYGS
                     KPSFILCSAT"
     gene            750..2024
                     /locus_tag="P5313_13475"
     CDS             750..2024
                     /locus_tag="P5313_13475"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRY81425.1"
                     /translation="MSKVLSIVLGGGKGTRLYPLTKDRSKPAVPFGGKYRIVDIPISN
                     CINAGFKQIYVLTQFNSASLHLHVARTYMFDDFSEGFVEIMAAEQTLTHSGWYEGTAD
                     AVRKNFIHFRTQNPTHYLIVSGDQLYRMDLDDMFRKHVESGCGLTIACTTVTREAASA
                     LGIMKVDKNHRIVEFQEKPGPVKDISDYRIPQELIADRRSGGKDFLGSMGIYIFDASL
                     MESSLDNDLMDFGKEVIPKVLSENPVNAYIFNGYWEDIGTIRNFYDANLDLTEINPPF
                     NFYEEQKPIFTHRRNLPASKINYCTLDRTLTADGCIITNASLSRSIVGIRTIIETGAN
                     LDGTICMGADYYESDAQKAENARKGIPNIGIGHGCILKGAIVDKNARIGENCRIGIDA
                     MERKDGEYQAWYVVDGVIVIPKNAIIPPGTVI"
     gene            2031..2723
                     /locus_tag="P5313_13480"
     CDS             2031..2723
                     /locus_tag="P5313_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HRY81426.1"
                     /translation="MTRPDFALLVVDLQRYYIDPRASFRAWTEFHAPGTLAYIEGRIR
                     ESVVPAVSRLKAEFGRRGWPTAYLRLCGTAEDRSDLHRIFRQAHDLAGSEGFPDLYPL
                     ASDPLSEILPEAAPEPGDAVFDKTTFSGFASGGLVSWLRETAPRAIVFAGLATSQCVD
                     TTARDASDRGFGVVHIEDAQADYGADEHYASLFASRGVCGGVILSSGEFCADPDAILS
                     RMDAGTSRDRSD"
     gene            complement(2817..3332)
                     /locus_tag="P5313_13485"
     CDS             complement(2817..3332)
                     /locus_tag="P5313_13485"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRY81427.1"
                     /translation="MPQTKVSVFEYAIRSEEEGAAFYSNAARSLPAGPEKDFLAGLAV
                     EESRHAEVFRQLREKVAKKGVAELFGSVEVSDYLDAVVRGGLFESARGAVPGGAAPKT
                     VEDVYAIAIRAERSSILLYGELAGQATDKTARKIFEDLVREEKNHLVRIVALRADHDT
                     LFAIERFGCVC"
     gene            complement(3422..5038)
                     /locus_tag="P5313_13490"
     CDS             complement(3422..5038)
                     /locus_tag="P5313_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRY81428.1"
                     /translation="MKPAFLLPRSVAAVVRSILVASAVLSLSCGGAGNRISGVGEAEA
                     SVERPGAADSSGWYPYPGPVRLDERSDVALEAGPGFSDVLSIGPDLLVRDSFGRLSLY
                     SAELGRFLPWRGPDTERGTAAIVVAIAADSARIYVSQEDGFVHAFPAPSGSGPETEPV
                     KPLWSVHPGMVSDWILAVPERLVCAASDGKIAVLSASEGTVQAFRDLGLPLVGKPVAA
                     GSVLAAARSVGLAALGLPDLEFLWSGDRAPSNLPVLRTVRNLLAFQDHEGILRMLDAR
                     TGTELYSISGVQGSALACDGERWYVAGPDDSLGAFGFSDGTPVWTAGAAAERVSLGAG
                     MTRYLPRLAVDSERVYVVHSGGLESWNSRTGELIERSKVPGPVDGLFIAPGRLFCRMR
                     DGTLRAYGPESTQADLLDPQGAIRPDPAVIQRILSQLERYAEPQTAVRLAWRVYVPEA
                     VPSPDNRFTVFRYEVGEAGKKTFSLRPDGQDRMLVAVFDATGEERHANVGELGVDASF
                     EYWTEAGTWYVAVGNLRGQVPEAPVFLDIR"
     gene            complement(5035..6804)
                     /locus_tag="P5313_13495"
     CDS             complement(5035..6804)
                     /locus_tag="P5313_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81429.1"
                     /translation="MKALTRTCRVLLAAGVFFLSSVPRALPEGFDYLPVKEAQRARQI
                     QAKLDASLETWIALERRYEGEVQTARLSVSGIEDSKKVRVILEAGKSGAWTAGTGLSL
                     ARARGDALEQARLLASVLEPRLDRLGREALADLYRESLGRARRVPGSVSDYLDFAEKV
                     AAVDGAGQRYVVRALLPLAPESVGLRLAPDDGPPAAYAGSPELSEEAVRIRRLRKALE
                     ECRRAAPALGVPELPWSQADSEAAESFARALSLLEPEFRAHVFESAKIPGLRLLPAFL
                     AGLSPEEREGWARNRNVSLRAVDLLLGCASASLTSALEVPETASAQATVSAVIALSEL
                     ERDLAKGTSGIALLARLKDPALRRLLRSESAPPSVRKAVSEARNRLAEGLETDLSRIS
                     PIARHRVELEEVPGLAGYTAIHAFSSSLEGTWSPLTPESVQSTLDRFLSGFRAPIGGF
                     VSALEIGLIAGSASSVPEDPSSQGGFVPRKIIAQHLALSAEALSPSAYAALCVYLLEA
                     FRESARSAGVPPAFLTPPALAGLAATCEARCRGVVEDSQVALSLEQAGGRDGLLGLWE
                     EAAFNLESTSRALDVHADRKESP"
     gene            complement(6824..>7873)
                     /locus_tag="P5313_13500"
     CDS             complement(6824..>7873)
                     /locus_tag="P5313_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81430.1"
                     /translation="ESWEPVPGRRSGVSAEGYSGPLFIVQGSALVLRNLTIEADRTGD
                     FTFAESSEGSLSVDNLRIRVHATGELVVFRSRDSSLDGSDLEVEISGALFARVVEAAG
                     GETRIRGLTIRAERSLGYLTAFSFAGGSAEISRTRSESRTSGGFTLVRGVGSRFELRD
                     SYINARGSGYCEAFRLEGSRSAFYAVSADLDCAGPLTFASVEGGRIDVLHSSVALRGG
                     SVVFLDLKSAAFRLGNSILQDDSGVGMLLRTDEAVQRGAVVRNALSGFRAYLDGPVPA
                     ATVDALNRIAAPPDGQNFQETFRRSQDPGSLGLPLLLPGSAGFGGAYPVMIPPGDPDR
                     PEPARKDVGAVEGAL"
BASE COUNT         1501 a         2636 c         2480 g         1256 t
ORIGIN      
        1 atggtcgcgg agcagaggat gaacgagggt ttcgaaccgt aaaacgccgc gatccgtttg
       61 agcctccgga tcacgttggc cacgtggctg ccgaagatgc cccgataccc gtgcatctcg
      121 tccaccacca cgtagcgcag gcccgagaag aaccgcgacc acttggcatg gttgggcagg
      181 acgccggaat ggagcatgtc cggattggtg atgacgatgc ggcccgtatc ccgtgccgat
      241 acccgcaccg aactgggggt gtccccgtcg taggtggcga tcttgacggg gaggtcgcct
      301 cccagggcta cctcgttcaa ctcggcctgc tggtcctggg acaaggcctt ggtcgggaac
      361 aggtacaggg ctcgcgccga gggatcttcc aggagggcct gtacgaccgg gaggttgtag
      421 cagagggtct tgccgctggc cgtcggggtc accacgacga agttccggcc ggcccgcgcc
      481 aagtcccagg atgcgcgctg gtgcgtatac agacgctcga tgccccttcg agccagggcg
      541 cccgccaggc ggccgtcgag gtccggaggc aggggggcga aggaaccggg ggcctcgggg
      601 agcctctcga cccgggcgac gcagggagct atgtcgggat ccgcgagaat tctctcgaat
      661 acccggatgg cgggatcggg attcatgatt tatactacca tacgaagggt tcggtagtaa
      721 cgatatctga atatggaaag gagtccatcg tgtccaaggt attgtccatc gttttgggag
      781 gcgggaaggg aacacggctg tatcctctca cgaaggaccg ctccaagccg gccgtgccgt
      841 tcggaggcaa ataccggatc gtggacatcc ccatttccaa ctgcatcaac gcgggattca
      901 agcagatcta cgtgctgacg cagttcaatt ccgcctccct gcacctccac gtggcccgga
      961 cctacatgtt cgacgatttc tccgagggat tcgtggagat catggcggcc gagcagaccc
     1021 tcacccactc ggggtggtac gagggaaccg cggacgcggt gcgcaagaac ttcatccatt
     1081 tccgaaccca gaatcccacc cactacctca tcgtatcggg cgaccagctg taccgcatgg
     1141 atctggacga catgttccgg aagcacgtgg agtccggttg cgggctcacc atcgcctgca
     1201 ccaccgtcac ccgggaagcg gcctccgccc tgggcatcat gaaggtcgac aagaatcacc
     1261 ggatcgtcga gttccaggag aaaccgggac ccgtgaagga tatctcggac tacaggatac
     1321 cccaggaact catagcggac cgaaggtcag gagggaagga cttcctcgga tccatgggca
     1381 tctacatctt cgacgcgagc ctcatggaat ccagcctgga caacgacctc atggatttcg
     1441 gcaaggaggt gatccccaag gtgctgtccg agaatcccgt gaacgcctat atcttcaacg
     1501 gctactggga ggacatcggc acgatccgga acttctacga cgcgaacctc gacctgacgg
     1561 agatcaatcc gcctttcaat ttctacgagg agcagaagcc gatcttcacc catcgccgca
     1621 atcttccggc ctccaagatc aactactgca ccctggaccg caccctgacc gccgacggct
     1681 gcatcatcac caacgccagc ctttcccgct cgatcgtggg aatccggacc atcatcgaaa
     1741 ccggggccaa cctggacggg accatctgca tgggggcgga ctactacgag tccgacgccc
     1801 agaaggcgga gaatgcccgg aagggaatcc cgaatatcgg catcggacac gggtgcatcc
     1861 tcaagggcgc catcgtggac aagaacgccc ggatcgggga gaactgccgc atcggcatcg
     1921 acgccatgga acggaaggac ggggaatacc aggcctggta cgtggtggac ggggtcatcg
     1981 tgatccccaa gaacgcgatc atacccccgg gaaccgtgat ctaaccgcgg atgactcgac
     2041 cggatttcgc gctcctcgtc gtggacctcc aacggtacta catagatccg agggcttcct
     2101 ttcgcgcgtg gaccgagttc cacgcccccg gcaccctggc gtatatcgag ggccgcatcc
     2161 gggaatccgt ggttccggcc gtgtcgcggc tcaaggcgga gttcgggcgg cgcggctggc
     2221 cgacggccta cctgaggctt tgcgggacgg cagaggatcg ttccgacctc cacaggatct
     2281 tccggcaggc ccatgacctg gcgggatcgg agggattccc ggatctatat ccgctagcct
     2341 cggatcccct ctcggagatc ctgcccgagg ccgccccgga acccggggac gccgttttcg
     2401 acaagaccac cttcagcgga ttcgcttccg gaggactggt ctcctggctc cgggaaacgg
     2461 cccccagggc gatcgtgttc gcggggctgg cgaccagcca gtgcgtagat acgaccgccc
     2521 gggacgcttc ggaccggggc ttcggcgtcg tccatatcga ggacgcccag gccgactacg
     2581 gggccgacga gcactacgcg tcccttttcg cctcccgggg ggtctgcgga ggcgtcatcc
     2641 tgtcgagcgg ggagttctgc gccgaccccg atgcgatcct gtccaggatg gacgcgggga
     2701 cctcgaggga ccgttcggac taaggtggac cggccggccc cctgctccgc cggaaaccga
     2761 gcggcccacc cgcccggccc ccggaattcc gcatccgtga tagacttaag ggaaactcag
     2821 cacacgcatc cgaaccgctc gatggcgaac agggtgtcat gatccgcccg gagggcgacg
     2881 atccggacga ggtggttctt ctcctcccgc accaagtctt cgaagatttt gcgggcggtc
     2941 ttgtccgtgg cctgtcccgc gagttcaccg tagagcaata tcgaactgcg ttccgctcga
     3001 atggctatgg catagacgtc ttccacggtc ttcggagccg ccccgcccgg taccgccccg
     3061 cgggcggact cgaacagacc tccccgcacc accgcgtcca ggtagtcgct cacttccacg
     3121 gagccgaaca gctccgcgac gcccttcttg gcgacctttt ccctcagctg acggaagacc
     3181 tccgcatgcc gggactcctc cacggccaag cccgcaagaa aatccttctc ggggccggcc
     3241 ggaagcgagc gagccgcatt cgaatagaag gcggcacctt cctcctccga acggatcgcg
     3301 tactcgaaaa cgctcacctt cgtctgcggc atgggggcct cctcataaca ggaagttttc
     3361 gcgatgtagc cgggtctgtc aaccatccga catcgagctc tgtcccccga atccggagga
     3421 atcaccgtat gtcgagaaaa acgggcgcct cgggtacctg gcccctcaag tttccgaccg
     3481 cgacgtacca ggtgccggcc tcggtccagt actcgaagga tgcgtccacg ccgagctcgc
     3541 ccacgttggc atgcctctcc tcgcccgtcg cgtcaaagac cgccacgagc atccgatcct
     3601 gcccgtcggg cctcagggaa aaggtcttct ttccggcttc cccgacctcg tatcggaaca
     3661 cggtgaaccg attgtccggc gagggaacgg cctcgggaac gtagacccgc catgccagac
     3721 ggacggccgt ctggggttcg gcatatcttt cgagctggct caggatccgc tggatcacgg
     3781 ccgggtccgg ccgaatcgcg ccttggggat ccagaagatc cgcttgggtc gattctggac
     3841 cgtaggcgcg gagagtacca tcccgcatcc tgcagaacaa cctgccgggg gctataaaaa
     3901 gcccatccac gggaccgggc accttgctcc gttcgatcaa ttcgccggtc ctggaattcc
     3961 aggattctaa accgccggaa tgaaccacgt agacacgttc ggaatccacc gcgagcctgg
     4021 gaagatatcg agtcatcccc gccccgagag atacacgttc ggccgcggcc cccgcggtcc
     4081 agacgggcgt cccgtccgag aatccgaagg cgcccaagct gtcgtccggc cccgcgacat
     4141 accaacgttc cccgtcacaa gcgagggccg atccttgtac gccggagatg ctgtagagct
     4201 ctgtccccgt tcgggcatcc agcatccgca agatcccctc gtgatcctga aacgccagga
     4261 ggttacggac cgttcgcagg accggcaggt tcgatggagc tctgtccccg ctccaaagga
     4321 attccaggtc cggaaggccg agtgctgcga gtccgacgga ccgggccgcc gccaggacgg
     4381 aacccgccgc gacgggcttc cccaccaggg gaaggcccag atcccggaag gcctggacgg
     4441 tcccctcgga agccgagagc acggcgatct ttccgtccga agcggcgcac acgagacgtt
     4501 cggggacggc caggatccaa tcggagacca tacccggatg tacggaccag agaggcttga
     4561 ccggttcggt ttccggaccg gatcccgagg gcgcgggaaa cgcgtgtaca aagccgtctt
     4621 cctgggaaac gtagattctc gcggaatccg ccgcgatagc gaccacgatc gcggctgtcc
     4681 cgcgttcggt gtccggtccg cgccaaggga ggaagcggcc aagctccgcg gagtacaggg
     4741 aaagtcgtcc gaaactatcc cggacaagga gatccgggcc gatcgacagg acatccgaga
     4801 atcctggacc ggcttcgagc gccacgtccg agcgttcgtc caggcggacc gggcccgggt
     4861 atgggtacca gccggaggaa tccgcggcgc ccggcctctc gaccgaggcc tccgcctccc
     4921 cgacgccgct gatccgattc ccggcacccc cgcaggaaag ggacagtacc gccgaggcga
     4981 ccaagatgct ccgtacgacc gcggccaccg acctcggaag aaggaaagcc ggcttcatgg
     5041 gctctccttc ctgtcggcat ggacatccag ggcccgactt gtggactcca ggttgaacgc
     5101 ggcctcttcc cagagcccga gcaggccatc ccgtcccccg gcctgttcga gggacagagc
     5161 cacctgagag tcctccacca cccccctgca tcgagcttcg caggtcgccg cgaggccggc
     5221 cagtgcggga ggagtcagga aggccggggg aacgccggcg ctccgggcgg attcacggaa
     5281 ggcctccagc agatatacgc acagcgccgc gtaggccgac ggggacagag cttcggccga
     5341 caaggccagg tgctgagcta tgatcttccg ggggacaaag ccgccctggg atgatggatc
     5401 ttcggggaca gagctcgccg atccggcgat caagccgatt tccagcgcgg atacgaaacc
     5461 tccgatcggg gcgcggaaac cggacaggaa tcgatccagg gtggattgaa cggattcggg
     5521 cgtcaaggga ctccacgtac cctcgaggct cgatgagaag gcatggatcg ccgtgtatcc
     5581 cgcgaggccg gggacttcct ccaactccac ccgatggcgt gctatcgggg aaatcctgga
     5641 cagatcggtt tccaggccct cggccagccg gttcctcgcc tccgaaacgg ccttccggac
     5701 gctcgggggt gcggattcgg accgaaggag ccgccgcagg gcgggatcct tcaagcgggc
     5761 aagcagggcg attccggacg ttcccttggc caggtcccgc tccagctccg agagggcgat
     5821 cacggcggag accgtcgcct gcgcggatgc cgtttccggg acctcgagcg cggatgtaag
     5881 gctggcggac gcgcagccta gcaacaagtc caccgcccgc aaggacacgt tccggtttcg
     5941 tgcccaccct tcgcgctcct ccggcgaaag gcccgccagg aaggccggaa gcaggcgcaa
     6001 gcccgggatt ttagcgcttt cgaatacatg cgcccggaat tcgggttcca ggagggacag
     6061 agctcgggcg aaactctccg cagcctcgct gtccgcctgc gaccacggca gttcgggcac
     6121 cccaagggca ggcgccgccc gcctgcattc ttccaatgcc ttgcgcaacc gccggattcg
     6181 cacggcttcc tcggacagct ccggggaacc ggcatatgcg gccggaggcc cgtcgtccgg
     6241 tgccagccgg agtccgaccg actccggagc cagggggagc agggctcgca cgacgtatcg
     6301 ctgccccgcg ccgtcaacgg cggccacctt ctccgcgaaa tccaggtaat ccgacaccga
     6361 tccggggacc cggcgggccc tccccagcga ttcccggtac aggtccgcga gagcctcccg
     6421 gccgagccgg tcgagcctcg gctccaggac ggatgccaag aggcgggcct gctccaaggc
     6481 gtccccccgg gcacgggcca ggcttaatcc ggtgcccgcg gtccaagcgc cgctcttccc
     6541 cgcttccagg ataaccctca cctttttcga gtcctcgatg ccggatacgg agaggcgggc
     6601 cgtttgaacc tcgccctcgt agcgacgctc cagggcgatc caggtttcca gggaagcgtc
     6661 cagcttggct tggatctgac gcgcccgctg ggcttccttg acgggcaggt aatcgaagcc
     6721 ctccggcagg gcccggggga cagagctcaa aaaaaacacc cccgcggcca gcagcacacg
     6781 gcaggttcga gtcaaagcct tcatccgacc ttccggaagc gcatcatagg gcgccctcga
     6841 cagcccccac gtcctttcgc gccggctccg ggcgatccgg gtctccgggc gggatcatga
     6901 cgggataggc tccgccgaag cctgcggaac ccggaagcag gaggggcagt ccgagacttc
     6961 ccggatcctg ggaccttcgg aaggtttcct ggaagttctg accgtccggc ggggctgcga
     7021 tccggttcag ggcatccacc gtcgcggcgg gaacgggccc atccaagtat gcacggaatc
     7081 ccgagagggc gttccgtacc acggcccccc tctgcacggc ttcatccgtc ctcagaagca
     7141 taccgactcc cgaatcatcc tggagtatcg agtttccgag gcggaacgcg gcggacttga
     7201 gatccaggaa gaccacggag cctccgcgca gggcgaccga ggagtgaagc acatcgatcc
     7261 ttcctccctc cacggaagcg aaggtcaagg ggccggcgca atccagatcc gccgagaccg
     7321 cgtagaaggc cgaccgggaa ccttccagcc ggaaagcctc gcagtaaccg gatccccgcg
     7381 cgttgatgta cgaatccctc aactcgaatc ggcttcccac cccccggacc agggtgaaac
     7441 ctccggatgt gcggctttcg gatcttgtcc tggaaatctc cgcggatccc ccggcgaagg
     7501 agaaggccgt gaggtatccc agggaccgct cggcccgtat cgtgaggccc cggatccggg
     7561 tctcccctcc ggcggcctcg accacccggg cgaagagggc gccggagatt tccacctcca
     7621 agtcggagcc gtccagggag gaatcccggg atcggaagac gacgagctct cccgtcgcgt
     7681 ggacccggat gcgcaggttg tcgacggaga gggaaccctc ggaggactcc gcgaaggtga
     7741 agtcgcccgt cctgtccgcc tcgatggtca ggttccgcag gaccagggcg ctgccctgta
     7801 cgatgaagag gggtccggag tatccctcgg ccgaaacccc gctgcgccgt cccggcacgg
     7861 gctcccagga ctc
//
LOCUS       DAOTEI010000164         7866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6530_length_7866_cov_4.781206, whole genome shotgun sequence.
ACCESSION   DAOTEI010000164 DAOTEI010000000
VERSION     DAOTEI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6530_length_7866_cov_4.781206"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1068
                     /locus_tag="P5313_13505"
     CDS             70..1068
                     /locus_tag="P5313_13505"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRY81431.1"
                     /translation="MSVKPVPPELPAFIDGREVFLIVGHKEPDGDCIGSQLALSSFLR
                     RKGKRTALLSSGPFTRTEILQYEHAFLDRIPDGLPLSRAALIVLDCSSLSRVGRIADQ
                     LPTLPVAFIDHHAAGEASGDVLYIDPAASSVTRLIHALIEAMGEEPTREEAELLFFGL
                     ATDTGFFRHLDAGGSDSFLSAARLVDRGASPKRTYSMIYGGKSLFTRKLMGEILVRTE
                     PFFDGRLLVSYVTEEDHRRYGIASRDSDMLYQLLMTVAGVEAAVVIRQESENNCTVGF
                     RSRDTVDVGAVAAQFGGGGHRLAAGLSIEGRIEDVRERMLEAFDRLFVTKGNDSDY"
     gene            1201..2058
                     /locus_tag="P5313_13510"
     CDS             1201..2058
                     /locus_tag="P5313_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81432.1"
                     /translation="MEQETFSCIARLADSGEGLREALGEMARILYRNPAAYGLKDADD
                     VGELFARYPGRIEAILRKYRETQGYFPAYLVATVRYLALSLRRDRARAFDRQMVFEEE
                     AVMCAEGVPGPEYGPAELESPLPESRGRGRYRGRSLRSRFLFLSVKCAWTADEDLLLR
                     AARALDLDTARLSKSLSRARARTLSVRDRWEERRTARDASWVRLRVLERRLSREEDPC
                     LRAVFRERIDRERERFRRRLEETRRLRLTISNKAVAEILGVPKGTVDAGMYNLARRLR
                     DFPFEGQNG"
     gene            2069..2665
                     /gene="rdgB"
                     /locus_tag="P5313_13515"
     CDS             2069..2665
                     /gene="rdgB"
                     /locus_tag="P5313_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRY81433.1"
                     /translation="MTLYIATNNAHKVKELAELFPGIPLRTPADAGIEFDHDETEDSF
                     LGNSLGKARALFGLVKAPVLADDSGLCVDALGGAPGVRSARYGSPDDGRTVLSARDKN
                     ELLLREMEGRRDRTCRFVCAMTLMLSEHRFVTVQETCEGRLLESSRGAEGFGYDPVFF
                     LPGEGLSMAELSLERKNRLSHRGRAAARILAVLASLPD"
     gene            complement(2745..2948)
                     /gene="rpmE"
                     /locus_tag="P5313_13520"
     CDS             complement(2745..2948)
                     /gene="rpmE"
                     /locus_tag="P5313_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRY81434.1"
                     /translation="MKKGIHPKYELTTITCACGNVIETRSTVKNLQVEICSACHPFFT
                     GKQKLIDTAGRVERFRKKYGIKE"
     gene            complement(3043..4698)
                     /gene="rho"
                     /locus_tag="P5313_13525"
     CDS             complement(3043..4698)
                     /gene="rho"
                     /locus_tag="P5313_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRY81435.1"
                     /translation="MAIIRKKRPVDIAAETEEPGQAEFPLESDLNLTEESQGSFEPGP
                     EAPEPREEGADKEPVRIRVRRKPGREVPPADQVSPEQPRPEAAREDSPGFRTTTISVP
                     GFVRKQEAVEKPSEPSRSDHVDSRSRLSINDLTSMPLKELRDLGSKYGIGHEEMISLK
                     KQELIFSTLKAHTERGGIIYAYGSLEILPDGYGFLRSPQNSYLPGSDDIYISPSQIRL
                     FNLKTGDTVYGQIRSPKEGERFFAMLRVEQVNFDEPAVAQNRIPFENLTPLYPNQRLN
                     LETDTKEISTRIINLFCPIGKGQRALVVSPPKTGKTILLQRIANSITANHPEVYLIVL
                     LIDERPEEVTDMERTVKAEVISSTFDEQATRHVQVAEMVLEKAKRLVEHGKDVVILLD
                     SITRLARAYNQTVPTSGKILSGGVDSNALHKPKRFFGAARNIEGGGSLTIIATALIDT
                     GSRMDEVIFEEFKGTGNMEINLDRRLSDRRTFPAFNIKKSGTRKEELLLTDEELQKIW
                     VLRKVINPMDDIEIMELLIDRMMKSKNNEVFLKSMNTPYNGND"
     gene            complement(4897..5430)
                     /locus_tag="P5313_13530"
     CDS             complement(4897..5430)
                     /locus_tag="P5313_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRY81436.1"
                     /translation="MRPSARGSEAGEPAKAAAARLLEDLSRRGWTAALAESCTGGLVS
                     AALTSIPGASDHFWGAVVSYANEAKVKVLSVPRDVLERHGAVSAETVRAMARGVLDLS
                     GADVSASVSGIAGPGGGSPEKPVGTVWIGLACRDGRSRELRLDLTGGRDRIRGDSALR
                     VLGELRSFVEGGSAGSS"
     gene            complement(5430..5822)
                     /locus_tag="P5313_13535"
     CDS             complement(5430..5822)
                     /locus_tag="P5313_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY81437.1"
                     /translation="MPKNASKTIHEISTILGRETGFSGKLKFQESLMIRGNFEGEIDA
                     QGFLHIDEGAEVRAGLIRASSIVVGGVVHGDLEAADRIELLPTAKVYGKIRTTKLRIA
                     DGVIFEGRCEMIRNPSDFSPFATRAEPS"
     gene            complement(5833..6054)
                     /locus_tag="P5313_13540"
     CDS             complement(5833..6054)
                     /locus_tag="P5313_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81438.1"
                     /translation="MITEEKKRVLELFAQGRKLYKLMKFKEAEPLFAEALRIDSLDGP
                     AKVYLERCRHYMQEPPPEDWDGVFVMKTK"
     gene            complement(6070..6732)
                     /locus_tag="P5313_13545"
     CDS             complement(6070..6732)
                     /locus_tag="P5313_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY81439.1"
                     /translation="MDDKKRILIVDDEQINLEFFDVMLSKLGFRVEKAENGMEALDSI
                     RKEKPDLVILDNVMPKLSGWEVTRLLKTNPDYSEYADIPIIMFSAMDDVKDKVEGLEL
                     GADDYITKPFNFSEVLARIRAVLRTRELIRQIEYREGRLRIAEEMAGAAVQVSSEVRP
                     LLDALGSRSGAPAEEVRKIVSKLDEWERRVESWKAEADRLKTGETALAAIKRKVREHS
                     GQ"
     gene            complement(6785..>7866)
                     /gene="lnt"
                     /locus_tag="P5313_13550"
     CDS             complement(6785..>7866)
                     /gene="lnt"
                     /locus_tag="P5313_13550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRY81440.1"
                     /translation="RGRPGTGPQRDRKPALASAAAAVAIVVLSYASGAPLRTDWEPRG
                     YLRAALVQPAFRERQRSIRDYRAMADRLIRLTEEVRPEKPDLVVWHETAIVPPLGWHL
                     RFRPNRDTLDLAMRLDGYIRGLDVPLLFGNGRAEPSGPDTGLRRNWNSAFLFDRGREA
                     ARYDKMRLVPYSETFPPKKVLPGIAALIEAEVGQFWEAGREITVFRVGDAKFSVPICF
                     EDSFGDHTRAMAAAGAEFLVVLTDDSWGKSASCQYYHLTQSALRAAELGIPIARAANT
                     GSTALLGPDGTLRTELPPFEEGILRVDIPLPPASFRGTGYRRIGDAVGWAALALGAAW
                     AAASAAIAAAACTAGKRPGIRKTLD"
BASE COUNT         1204 a         2544 c         2653 g         1465 t
ORIGIN      
        1 gggacccggc gggacggccc agtctcctga gtcaccccgt ctgtccgccg gccgcttgaa
       61 aggcgtacca tgagcgtcaa acccgttcct cccgagctgc cggccttcat cgacggccga
      121 gaggtattcc tgatcgtcgg gcacaaagag cccgacggag actgcatcgg atcccagctg
      181 gccctgtctt ccttcctgcg gaggaaggga aagaggacgg ccctcctgtc ctcgggcccc
      241 tttacccgga cggagatcct gcagtacgaa cacgccttcc tggaccggat acccgacggt
      301 cttccccttt cccgggcggc ccttatcgtc ctggactgct cctccctctc ccgggtgggg
      361 cggatcgccg accagcttcc aacccttccc gtcgccttca tcgaccacca cgcggccggg
      421 gaggcttcgg gggacgtcct ctatatcgat ccggcggctt cctcggtcac ccgactgatc
      481 cacgccctga tcgaggcgat gggggaggag ccgacccggg aggaggcgga gctcctcttc
      541 ttcggcctgg ccacggacac gggcttcttc cgccacctgg acgcgggggg ttcggactcc
      601 ttcctgtccg cggcccggct ggtcgatcgg ggagcctccc ccaagcgtac ctattcgatg
      661 atctacgggg gcaagtccct gttcacccga aagttgatgg gcgagatcct ggtccgcacg
      721 gagccgtttt tcgacggccg cctcctggtt tcctacgtga ccgaggagga ccataggcgc
      781 tacggcatcg ccagccggga ttcggacatg ctctaccagc ttctcatgac cgtcgcgggg
      841 gtcgaggcgg ccgtggtcat ccggcaggaa agcgagaaca actgcaccgt aggattccgg
      901 tcccgggata ccgtggacgt cggggccgtc gccgcccagt tcggcggagg cgggcatcgg
      961 ctcgccgcgg ggctttccat cgagggccgg atcgaggacg tgcgggagag gatgctggaa
     1021 gccttcgacc ggctatttgt aacaaaaggt aacgactcgg actattgatt tccggtatgc
     1081 cggagatccc cctccgtaat tccatatccg gactcggccg tttcgaggag ccagacggac
     1141 ctcgaacctt ggcatggaac ttgccatgtt ctcctcgccc gcaggggccg ggagggaggc
     1201 atggaacagg agaccttttc atgcatagcg cggctggcgg attccggcga aggacttcgg
     1261 gaagccctgg gagagatggc ccggatcctg tataggaacc ccgccgcgta tggcttgaag
     1321 gacgcggacg atgtcggcga gctcttcgcg aggtatccgg gcaggatcga ggccatcctg
     1381 aggaagtacc gggaaaccca gggatatttc ccggcttatc tcgtcgccac cgtccggtac
     1441 ctggccttgt cgctccgcag ggaccgggca agggccttcg accgccagat ggtcttcgag
     1501 gaggaggccg tgatgtgcgc ggaaggagtt ccgggtcccg agtacggtcc cgcggagctg
     1561 gagtcccccc tgcccgaatc ccggggccgc ggacgctacc gcggccgctc cctccggtcc
     1621 cggttcctgt tcctgagcgt gaaatgcgcg tggaccgcgg acgaggacct cctcctccgg
     1681 gccgctcggg ccctcgatct cgacaccgcc cggctgtcga agagcctttc ccgggcccgg
     1741 gcgcgcaccc tctccgtccg ggaccgctgg gaggagcgca ggacggcccg ggacgcgtcc
     1801 tgggtccggc tccgggttct tgagcgtcgc ctgtcccgtg aggaggatcc ctgcctccgg
     1861 gcggttttcc gggaacggat cgaccgggag cgggaacggt tccgccggag gctcgaggag
     1921 acacgcaggc ttcggttgac gatttccaac aaggcggtcg cggagatcct gggcgtgccg
     1981 aaaggcacgg tggacgcggg gatgtacaac ctcgcccgga gactgcggga ctttcccttc
     2041 gagggacaaa acggctagta tcccccccat gacgctgtac atcgccacca acaacgccca
     2101 caaggtcaag gagctcgcgg agctcttccc ggggatcccg ctccggaccc ccgcggatgc
     2161 ggggatcgag ttcgaccacg acgagacgga ggacagcttc ctgggcaaca gcctgggaaa
     2221 ggcccgggct ctcttcgggc tcgtgaaggc cccggtgctg gcggacgatt cggggctctg
     2281 cgtggacgcc ctgggcgggg cgccgggggt gcggtcggcg cgctacggct ccccggacga
     2341 cggccggacc gtcctgtccg cccgggataa gaacgagctc ctcctgcggg agatggaggg
     2401 ccggcgggac cggacctgcc gcttcgtctg cgccatgacc ctgatgctct cggaacaccg
     2461 cttcgtcacg gtccaggaga cctgcgaggg ccgtctcctg gagagctccc ggggcgccga
     2521 gggattcggg tacgatccgg tctttttcct gccgggggaa ggcctctcga tggccgagct
     2581 ctccctggag cggaagaacc ggctgagcca ccgaggccgg gccgcggcgc ggatcctcgc
     2641 cgtcctggcg tcccttccgg actgaagggg ggggcaatga aaaggccgcg ggcgatgcgc
     2701 cgcggccttt cgacgaggaa cggcgccgaa ggcggcgcct cttcctactc cttgattccg
     2761 tactttttcc ggaatcgctc cacgcgtccc gcggtgtcga tgagcttctg tttgccggta
     2821 aagaaggggt ggcaggccga gcaaatttcg acctggagat tctttaccgt cgaacgggtc
     2881 tcgatgacgt tgccgcaggc gcacgtgatg gtggtgagct cgtacttggg atggatgccc
     2941 ttcttcacag taccctccgc gcctatctgg ctgcagcctg aaaaccggcc tctgacggta
     3001 gtccgcacgg agcgggcgat ggaggcccgc gtccggcttc cctcagtcgt tcccgttgta
     3061 cggggtgttc atggacttca ggaagacctc attattctta cttttcatca tcctgtcaat
     3121 gaggagctcc atgatctcta tatcgtccat gggattaatc accttgcgga gcacccagat
     3181 cttctggagc tcctcgtccg tcaggaggag ttcctccttg cgggtgccgg actttttgat
     3241 gttgaaggcc gggaaggtcc ggcggtccga gagccggcgg tccaggttga tctccatgtt
     3301 gcccgtgccc ttgaactcct cgaagatcac ctcgtccatg cggctgcccg tgtcgatcag
     3361 ggccgtggct atgatggtca ggctgccgcc gccttcgatg ttccgggcgg ctccgaagaa
     3421 gcgcttgggc ttgtgcaggg cgttggaatc cacgccgccc gacaggatct tgccgctggt
     3481 gggcacggtc tggttgtacg cccgggccag gcgggtgatg gagtccagga ggatcaccac
     3541 gtccttgccg tgctcgacca ggcgcttggc cttctccagg accatctccg ccacctggac
     3601 gtgccgggtg gcctgctcgt cgaaggtgga ggagatgacc tcggccttga cggtacgctc
     3661 catgtccgtc acttcctccg gccgctcgtc gatcaggagg acgatgaggt agacctcggg
     3721 atggttggcg gtgatggagt tggcgatgcg ctggaggagg atggtcttgc cggtctttgg
     3781 gggggatacc accagggcgc gctggccctt cccgatgggg cagaagaggt tgatgatccg
     3841 ggtggagatc tccttggtgt ccgtctccag gttcagccgc tggttcgggt acaggggggt
     3901 caggttctcg aaggggatgc ggttctgggc caccgcgggt tcatcgaagt tgacttgctc
     3961 cacccggagc atggcgaaga agcgttcccc ttccttgggg ctccggatct ggccgtacac
     4021 ggtgtccccg gtcttcaggt tgaacaggcg gatctgggac ggggagatgt agatgtcgtc
     4081 ggagccgggc aggtaggagt tctgggggga gcggaggaag ccgtagccgt ccggaaggat
     4141 ctccagggag ccgtaggcgt agatgatccc gccccgctcc gtatgggcct tgagggtgga
     4201 aaagatgagc tcctgcttct tgagggagat catctcctcg tggccgatcc cgtacttgga
     4261 gcccaggtcg cggagctcct tgaggggcat gctggtcagg tcgttgatgg aaagccggga
     4321 ccgggagtcc acgtggtcgg aacggctcgg ttccgagggc ttctcgacgg cctcctgctt
     4381 cctcacgaaa ccgggcacgg agatcgtggt ggtgcggaac cccggcgagt cctcccgggc
     4441 cgcctcgggg cggggctgtt ccggcgagac ctggtcggca ggcgggactt cgcggcccgg
     4501 tttcctacgg acccggatcc ggaccggttc cttgtccgcg ccttcttccc ggggttccgg
     4561 ggcctcgggg ccgggctcga aggaaccctg agactcctcc gtcaggttca agtccgactc
     4621 gaggggaaat tccgcctgtc cgggctcctc ggtctccgcc gcgatatcga cggggcgttt
     4681 ctttcggatt attgccatca atcactccgt actgaaggtt ggattctcgg tatgccgcgg
     4741 gccgccgcat cgggcgtcca cggggatcga atcgcgccga aaccctcggg ttggcgcgtt
     4801 tcgaagctgg atcttgtcag aagggggaac ttcgccggcc gggcctggtg ccctccgtgg
     4861 cggacatccc atcctatatc ccgatgcctc ccgctgtcaa gaagacccgg cggagccccc
     4921 ttccacgaag gaccggagtt cccccaggac gcggagcgcg gagtccccgc ggatccggtc
     4981 cctcccgccc gtcaagtcca ggcggagctc ccggctcctc ccgtcccggc aggccagccc
     5041 gatccacacg gtgccgacgg gcttttccgg gctccctccc ccggggcccg cgataccgga
     5101 aaccgaggcc gagacgtccg cgccgctcaa gtccaggacc cccctggcca tggcccggac
     5161 cgtctccgcg gaaacggctc cgtggcgttc caggacgtcc cgggggacgg aaaggacctt
     5221 taccttggcc tcgttcgcat aggagacgac ggccccccag aaatgatcgg aagccccggg
     5281 tatggaggta agggcagcgg agaccagccc gcccgtacag gattccgcca gggccgcggt
     5341 ccatccgcga cgggacaagt cctcaaggag tcgcgccgcc gcggccttcg cgggctcccc
     5401 ggcttccgac ccgcgggcgg acggtcgcat caggatggtt cggcccgggt cgcgaagggg
     5461 ctgaagtcgg aggggttgcg gatcatctcg cagcggccct cgaagatcac gccgtccgcg
     5521 atccgcagct tggtggtgcg gatcttgccg tacaccttgg cggtgggcag cagttcgatg
     5581 cggtccgcgg cctccaggtc gccgtggacc acgccgccga ccacgatgct ggaggcccgg
     5641 atgagcccgg cccggacctc ggcgccctcg tcgatgtgca ggaaaccctg ggcatcgatc
     5701 tcgccctcga agttcccgcg gatcatgagg gattcctgga atttcagttt tccggagaac
     5761 ccggtttccc ggcccaggat cgtgcttatc tcgtggatcg tcttggatgc gttcttgggc
     5821 atgggcttcc tgtcacttcg tcttcataac gaacacgccg tcccagtcct ccgggggcgg
     5881 ttcctgcata tagtgccggc agcgttccag atagactttg gccggcccat ccagggagtc
     5941 gatccggagg gcttccgcga acaggggttc ggcttccttg aacttcatga gcttgtagag
     6001 cttgcgcccc tgggcgaaga gttcgaggac ccgttttttc tcttccgtga tcactcccac
     6061 cctcctagct tactgtccgg aatgctcccg gaccttcctc ttgatggccg ccagggcggt
     6121 ctctcccgtc ttcaggcggt cggcctcggc cttccaggat tccacgcgcc gttcccactc
     6181 gtccagcttg gagacgatct tgcggacttc ctccgcaggg gcgcccgacc tggagcccag
     6241 ggcgtccaag aggggccgga cctccgagga aacctgaacc gccgccccgg ccatctcctc
     6301 cgcgatcctg agccggcctt cccggtactc gatctggcgt atcagttccc gggtccgaag
     6361 gacggcccgg atccgggcga ggacctcgga gaaattgaag ggtttggtga tgtagtcgtc
     6421 cgccccgagt tccagtccct ccaccttgtc cttcacgtcg tccatcgcgg aaaacatgat
     6481 gatggggatg tcggcgtatt ccgagtaatc cgggttggtc ttgagcaggc gggtcacctc
     6541 ccagcccgag agcttgggca tgacgttgtc caggatcacc aggtcgggtt tttccttccg
     6601 gatggagtcc agggcttcca tcccgttctc cgccttttcc acccggaaac cgagtttgga
     6661 aagcatgacg tcgaagaatt cgaggttgat ctgctcgtcg tctacgatta gtatgcgctt
     6721 cttgtcgtcc atctgtacct acttgcggtg tcgtgcctca ttttcctata gccgtccggt
     6781 attgtcaatc gagggtcttc cgaatcccgg ggcgctttcc cgcggtacac gcggcggccg
     6841 cgatcgccgc ggaggccgcg gcccaggccg cccccaaggc cagggcggcc cagccgacgg
     6901 cgtccccgat ccgccggtac cccgtgccgc ggaacgacgc cggaggcagg ggtatgtcca
     6961 cccggaggat cccctcctcg aagggaggca gctccgtccg cagggttccg tcgggcccga
     7021 gcagggccgt ggagccggtg ttggcggccc tggcgatcgg aatgcccaac tccgcggccc
     7081 tcagggccga ttgggtcagg tgatagtact ggcaggaagc cgattttccc caggaatcgt
     7141 ccgttaggac caccaggaac tcggcgcccg ccgcggccat agcccgggtg tggtctccga
     7201 aggaatcctc gaagcagatg ggcacggaga acttggcgtc tcccacgcgg aagacggtga
     7261 tctcccggcc ggcttcccag aattggccga cctcggcttc gatgagcgcc gctatgcccg
     7321 gcaggacctt cttcggggga aaggtttctg agtagggcac caggcgcatc ttgtcgtacc
     7381 gggccgcctc ccggccccgg tcgaacagga aggcgctgtt ccagttcctg cggaggccgg
     7441 tatccggccc cgaaggctcg gcccggccgt tcccgaagag gaggggcacg tccaggccgc
     7501 ggatgtagcc gtcgaggcgc atcgccaggt ccagggtgtc ccggttcggg cggaaccgga
     7561 gatgccatcc gagggggggt acgatggcgg tctcgtgcca gacgacgagg tcgggtttct
     7621 cggggcggac ttcctcggtc aggcggatca gccggtccgc catggcccga tagtcccgga
     7681 tggaccgctg gcgttcccgg aaggcgggct gcaccagggc cgcccggagg tatccccggg
     7741 gctcccagtc cgtccggagc ggggcgccgg aggcgtagga caggaccacg atggcgaccg
     7801 cggccgcggc ggacgcgagg gcgggcttcc ggtccctctg cggtccggtt cctggacgtc
     7861 cacgca
//
LOCUS       DAOTEI010000165         7861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6536_length_7861_cov_5.002818, whole genome shotgun sequence.
ACCESSION   DAOTEI010000165 DAOTEI010000000
VERSION     DAOTEI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6536_length_7861_cov_5.002818"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="P5313_13555"
     CDS             <1..1013
                     /locus_tag="P5313_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766714.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRY81441.1"
                     /translation="ADAFARAWFKLTHRDMGPKARYLGPEVPKEDLIWQDPLPARDYP
                     LIDSADIAALKKKVLAAGVPPADLVFVAWASASTFRGSDKRGGANGARIRLAPQKDWA
                     ANEPARLAKVLAALEAAKAGFDRQAAGGKQVSLADLIVLGGCAAVEEAARKAGFPVEV
                     PFRPGRVDARQDQTDPVSFSFLEPKADGFRNFRKEAYAVPDEELLLDRAQLLTLTAPE
                     MTVLVGGLRVLGANTGGSYHGVLTKRPGVLSTDFFVNLLDMGTEWKAAGKEPGLYEGL
                     GRGDGRLHWTATRADLVFGADSRLRALAEVYASDDAGEKFVRDFAAAWAKVMDLDRFD
                     LL"
     gene            1143..1571
                     /locus_tag="P5313_13560"
     CDS             1143..1571
                     /locus_tag="P5313_13560"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRY81442.1"
                     /translation="MNEGKRRILMAAGFLAFGLGWVGIFLPGLPTTIFWILAGLAFLR
                     TNRRMYEKIVSHRRFGPGVKLFVEEGRIRARGKIISIAAMMTFASLGALAIPPAWVKI
                     LVVSAAACGSLWVALLPTPEGAARAAAEAPDAEGKDAESA"
     gene            1653..2102
                     /locus_tag="P5313_13565"
     CDS             1653..2102
                     /locus_tag="P5313_13565"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRY81443.1"
                     /translation="MEILRKKEQPALSIRYRTAAAKLPETMGPVFGQIAAYMARKGIP
                     FAGPPFAMYHNMDMEDLDVEIGFPVGKAEPGEGRIKSGRLPGGPTATAKHTGPYTTIE
                     TTYNTLMAFVAEKGVTPESFMYEEYLNSPEDTPVEKLETNIYFPLKG"
     gene            complement(2158..2231)
                     /locus_tag="P5313_13570"
     tRNA            complement(2158..2231)
                     /locus_tag="P5313_13570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2195..2197),aa:Arg,seq:cct)
     gene            complement(2278..4740)
                     /locus_tag="P5313_13575"
     CDS             complement(2278..4740)
                     /locus_tag="P5313_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY81444.1"
                     /translation="MEQKDYRLAAILYTDIAGFSRMMENDEAGTLRLLHFHNAVITEA
                     AERRHGTVIKTIGDAFLVDFRNTVEALQTALEIQDKFYQYNLEHKELPLLVRVGIHLG
                     DIYFFENDALGEGINIAARLQSLAHPGAICMSQDVYNQVLNKIDFHAVKLGKVSLKNI
                     TKEIHAYEISSANVEFDPNRDKPQPGFPPGAYAGPEPAELGTLGPTLPEAPPKTSREA
                     GTGSPKPRGPDRDYSPEGSANVLSEIRKAILDDTRTEGRRLSVDEALKRYGDYGVEAH
                     EVIARMAEQGLLIKRTPSRTDGAGKFDGESLGRDIERAVRGIVALVDDKIGEYEESRR
                     RDRGSARDVGRDGNREDGRDRFERGMDRMEARLERFAERMERGAGRWSEKTAIKAELR
                     RHAETTETGEWDKELKDSDYFKPGREELATSFEAYREGLAEKARRLRGGFAGNLISFL
                     GVNALLWFINLQLQAGPPFLWAAIVTAGWGTGVVSNLAGLARTNAKLREADRVPDLEG
                     EALATYKKLNIVRDSMARHTASVLTVPALLFTIHAVATPLVFPWWLIPSGIMAISWVS
                     HAIAYASARRRLESKLFKEAGVGGWRDLFRIGKTRKAAARASGPYATLYAEAETAKEA
                     IVAQVKAAGKDSPFDKELIPSLEEYVGQVRLLTQTVNEIDSIIDAIPMKDLAKDKAEL
                     RGKLEGSVNSSLKSEYARSVEEIERQEKSFQDLQDQREVLHLRLKSSVNSLKQMQLDM
                     ARYRANPDLAQSASLDQVRSRTGELSKYLEDLRKGYEEAREAEDPWKDLERQAAEREA
                     RAKALPESEGSGNEGGADSPSR"
     gene            complement(4838..6649)
                     /locus_tag="P5313_13580"
     CDS             complement(4838..6649)
                     /locus_tag="P5313_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRY81445.1"
                     /translation="MIPNGALVFYKGKPALARNSGDKLELSIPGAPEVKVRPKDVELL
                     HPGPLSRVPEARPDGDFETAWEMSAGETLGLEALCELVFGRAGPEEALACRLEALEGL
                     RFRTEEAHPGFRAVSREEREKEAARRARKETEAADREAFLARARKGLFEPGDERFLGE
                     VEAFALGATQKARILGDLGLADKPEAVHAWLIKTGRWTDAVNPWPVRFGTASRAPDLE
                     LGPEDDDGRVDLTGLEAWAIDNAWSRDPDDAVSFDGRDVWVHVADPASAVLPGSPADR
                     EASDRAATLYLPEGSVPMLPDSALERFGLGLSGVSRALSVRISLEPDGSVREAEILPT
                     LVKVTRLSYGGADPLLGSGPLAELDRIASIRSEARTAAGAVDISIPEVRVWVDSGEPR
                     IDAIPEHRSGAVVREMMVLAGEAAARWAFDRSLPFPYYSQEAPQDAGSLPAGLAGEFA
                     KRRLMKAGMAGPQPRAHRGLGVPFYAQFTSPLRRYSDLLAHHQVRAVLAGRKPLGEDE
                     ISALLGRAAAAAALIRQAERASQLHWTLAFLGRRPGWTGEGVVVGTGNPASVYIPGLG
                     METRVRLGGTELNATVSLRLTGTDLARQEARFETAEA"
     gene            6964..7194
                     /locus_tag="P5313_13585"
     CDS             6964..7194
                     /locus_tag="P5313_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81446.1"
                     /translation="MVALIVGIVLILLAVVACVPFGLAWWQDVVVFLRGSLPVLAVLI
                     GLIAVFIGIADIKDRMEAKKEEEEEAKENAGK"
     gene            7426..7854
                     /gene="rplM"
                     /locus_tag="P5313_13590"
     CDS             7426..7854
                     /gene="rplM"
                     /locus_tag="P5313_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRY81447.1"
                     /translation="MKTVFVKPEEAPRSWYLIDAEGKSLGRVAAKVAYILRGKNKPFY
                     TPHQEIGDYVVVINADKVAVTGAKRENKMYYHHTGFPGGLKGQTYQALSDRHPGMPLE
                     IAIRGMLPKGPLGRKLNKNVKIYAGSSHPHAAQQPIALEL"
BASE COUNT         1200 a         2675 c         2696 g         1290 t
ORIGIN      
        1 tcgccgacgc cttcgcccgg gcctggttca agctgaccca ccgcgacatg ggtcccaagg
       61 cccgttacct cggcccggaa gtcccgaagg aagacctgat ctggcaggat cccctgccgg
      121 ctcgggacta cccgctcatc gactcggctg acatcgcggc gctcaagaag aaggtactgg
      181 ccgcgggtgt accccccgcg gacctcgttt tcgtcgcctg ggcctcggcc tcgaccttcc
      241 gcggttcgga caagcgcggc ggggcgaacg gcgcacgaat ccgcctggcc ccgcagaagg
      301 actgggctgc caacgagccg gcgcgccttg ccaaggtcct cgccgccctc gaggccgcga
      361 aggccggctt cgaccgccag gcggcgggcg gcaagcaggt ctccctggcc gacctgatcg
      421 tgctgggggg ctgcgccgcg gtcgaggagg ccgcccgcaa ggccggcttc ccggtggagg
      481 tgcccttccg gcccgggcgg gtggacgccc gtcaggatca gaccgacccg gtatccttct
      541 ccttcctgga gcccaaggcc gacggcttcc gcaacttccg gaaggaggcc tacgcggtcc
      601 cggacgagga actcctccta gacagggccc agcttctcac cctcaccgcg ccggagatga
      661 cggtcctcgt gggcggcctc cgggtcctgg gggccaacac gggcggcagc tatcatggtg
      721 tgcttacgaa gcggcccggc gtcctgtcga cggacttctt cgtgaatctc ctggacatgg
      781 gcaccgagtg gaaggccgcg gggaaggagc ctggtctcta cgaaggccta ggccgcgggg
      841 acggcaggct ccactggacg gcgacccgag ccgacctggt cttcggcgcg gattcgcgtc
      901 tccgggcgct cgccgaggtc tacgcctccg acgacgccgg ggagaagttc gtccgggact
      961 tcgccgcggc ctgggccaag gtgatggacc tggaccgctt cgacctgctc tagcgggggc
     1021 gccgcgccta cgcggcggct gtccggatcg gccgaacggc cttccgggcg gcccgccgga
     1081 gaccggccct ggactccggg gcggccggag tggtagcgtg gtaccgaagc ggggaggggc
     1141 ggatgaacga aggaaagcga cggatcttga tggccgccgg cttcctggcc ttcggcctgg
     1201 gctgggttgg aattttcctg cccggcctcc ccaccacgat tttctggatc cttgcgggcc
     1261 tggccttcct gcgcaccaac cggcgcatgt acgagaagat cgtctcccac aggcgcttcg
     1321 gtccgggggt caagctcttc gtggaggagg gcaggatccg ggcccggggc aagatcatct
     1381 ccatcgccgc gatgatgacc ttcgcgagcc tgggcgccct ggccataccc ccggcttggg
     1441 tcaagatcct ggtcgtgtcg gcggcggcct gcgggagtct ctgggtggcc ctgctgccga
     1501 caccggaggg cgcggcccgg gcggccgccg aggcgcccga cgcggaaggg aaggacgcgg
     1561 agtcggcctg aagatcgaag gcctgctccg cattccaggc tcgtgaaggc tgcggcagga
     1621 tagcctcatc ttcaaggtcg aggaggagcg ccatggaaat tctgcggaag aaggaacagc
     1681 ccgccctgtc gatccggtac cggaccgccg cggccaagct gcccgagacg atggggccgg
     1741 tcttcggcca gatcgcggcc tacatggccc ggaaggggat ccccttcgcg gggcctccct
     1801 tcgcgatgta ccacaacatg gacatggagg acctggacgt cgagatcggc ttcccggtcg
     1861 ggaaggccga gcccggggaa ggcaggatca agtcgggccg cctgcccggc gggccgaccg
     1921 cgacggccaa gcatacgggt ccctacacga cgatcgagac gacctacaac accctcatgg
     1981 ccttcgtggc cgagaaggga gtcaccccgg agtccttcat gtacgaggag tacctgaact
     2041 ccccggagga cacccctgtg gaaaagctgg agacgaacat ctacttcccc ctcaagggct
     2101 gattccccgg gaaatggaaa cggcccgcgg gcgtcgtgcc tgcggaccgt tcatgcctga
     2161 cccggacggg aatcgaaccc acgacctagc gcttaggagg cgctcgctct atccaactga
     2221 gctaccgggt ctagctttgg ggacaggtgc gatactaggg gcaaaacccg gggcttgtca
     2281 acgtgacggg gaatccgccc cgccctcgtt cccggagccc tccgactccg gaagggcctt
     2341 ggcccgggct tcccgttccg ccgcctggcg ctccagatcc ttccagggat cttcggcctc
     2401 ccgggcctcc tcgtagccct tccggaggtc ctccaggtac ttggagagct ccccggtgcg
     2461 ggagcgtacc tggtccaggg acgcggactg ggccaggtcg gggttggccc ggtaccgggc
     2521 catgtccagc tgcatctgct tgagggaatt gaccgaggat ttcaggcgaa ggtggaggac
     2581 ctcccgctgg tcttggaggt cctggaagga cttctcctgc ctctcgatct cctccacgga
     2641 ccgggcgtac tcgcttttca aggaggaatt gacgctgccc tcgagcttcc ccctgagctc
     2701 cgccttgtcc ttggccaggt ccttcatggg gatggcgtcg atgatggagt cgatctcgtt
     2761 gaccgtctgg gtgagcaggc ggacctgacc cacgtactcc tccagggacg ggatgagctc
     2821 cttgtcgaag ggggagtcct tgcccgcggc cttgacctgg gcgacgatgg cttccttggc
     2881 cgtctccgcc tcggcgtaca gggtggcgta ggggccggag gcccgggccg cggccttgcg
     2941 ggtctttcct attcggaaga ggtccctcca gcctccgacc ccggcctcct tgaagagctt
     3001 ggactccagg cgtcggcggg cggaggcgta ggcgatggcg tgggataccc agctgatcgc
     3061 catgatcccc gagggtatga gccaccaggg aaagaccagg ggcgtggcca cggcgtggat
     3121 ggtgaagagc agggccggga cggtgaggac gctggccgta tgacgggcca tggagtcccg
     3181 gactatgttc agcttcttgt aggtggccag ggcctcgccc tccaggtccg ggacccggtc
     3241 ggcctcccgg agcttggcgt tggtccgggc caggcccgcc aggttggaga cgaccccggt
     3301 cccccagccc gcggtgacga tggccgccca caggaagggc ggacccgcct gcagctggag
     3361 gttgatgaac cacaggaggg cgttcacccc caggaaggaa atgaggtttc cggcgaatcc
     3421 gcccctcagc ctgcgggctt tttccgccag gccctcccgg taggcctcga aactggtcgc
     3481 cagctcttcc cggccgggct tgaagtagtc cgagtccttg agctccttgt cccactcccc
     3541 ggtctcggtc gtctccgcgt gccgccggag ctcggccttg atggccgtct tctcgctcca
     3601 gcgtcccgcg ccccgttcca tgcgctcggc gaagcgctcc agccgggctt ccatgcggtc
     3661 catcccgcgt tcgaaccggt cccggccgtc ctcgcggttc ccgtcgcggc cgacgtcgcg
     3721 ggcgctgccg cgatcccggc gacggctctc ctcgtactcc ccgatcttgt cgtccaccag
     3781 ggccacgatg ccccggaccg cccgctcgat gtcccggccc agggattccc cgtcgaactt
     3841 ccccgccccg tcggtccggg aaggggtccg cttgatgagc agtccctgtt ccgccatgcg
     3901 ggcgatgacc tcgtgggcct ccaccccgta gtccccgtaa cgcttcaggg cctcgtccac
     3961 ggagaggcgc cgaccctcgg tccgggtgtc gtccaggatg gccttgcgga tctccgaaag
     4021 gacgttcgcg gagccttcgg ggctgtagtc ccggtcgggg ccgcggggct tcggggagcc
     4081 cgtgccggct tcccgggacg tcttcggcgg ggcttccggc agggtgggcc ccagggtccc
     4141 gagttccgcg ggctccgggc cggcgtaggc cccgggcggg aagcccggct ggggtttgtc
     4201 ccggttcggg tcgaattcca cgttggcgct ggagatctcg taggcgtgga tctccttggt
     4261 gatgttcttg agggagacct tgcccagctt gacggcgtgg aagtcgatct tgttgaggac
     4321 ctggttgtac acgtcctggg acatgcagat agcccccggg tgggccaggg actggaggcg
     4381 ggcggctatg ttgatgccct cccccagggc gtcgttctcg aagaaataga tgtcccccag
     4441 atggatgccg acgcggacaa ggaggggcag ctccttgtgc tcaaggttgt actggtagaa
     4501 cttgtcctgg atttccaggg cggtctggag ggcttccacg gtgttccgga agtccaccag
     4561 gaaggcgtcc ccgatggtct tgatgaccgt gccgtgcctc cgctcggcgg cctcggtgat
     4621 gacggcgttg tggaagtgga ggagccggag ggttcccgcc tcgtcgtttt ccatcatccg
     4681 ggagaaaccc gcgatgtccg tgtacaggat cgccgccagg cggtagtctt tttgctccat
     4741 tgacttggga ggatactccc gtggtcggcg gccgtcaagg acgcggggtt ccaccgttcc
     4801 ccgcagacgg ggcggacgga cgaaaccggc tccttcccta ggcctccgcg gtctcgaacc
     4861 gggcctcctg gcgggccagg tccgttccgg tcaggcgcag ggaaaccgtc gcgttgagct
     4921 ccgtcccccc caggcggacc cgggtttcca tccccaagcc ggggatgtac accgaggcgg
     4981 gattcccggt tccgacgacg acgccctccc cggtccagcc ggggcgcctt cccaggaagg
     5041 ccagggtcca gtggagctgg ctggcccgtt cggcctggcg gatcagggca gcggccgccg
     5101 cggcgcggcc caggagagcg gaaatctcgt cctctcccag gggcttgcgc cccgccagga
     5161 cggcccggac ctggtggtgg gcgaggaggt cggagtaccg gcgcaggggg ctcgtgaact
     5221 gggcgtagaa gggcaccccc aggccgcggt gggcccgggg ctgggggccc gccattccgg
     5281 ccttcatcag cctgcgcttg gcgaactccc cggccaagcc cgccggcagg gagcccgcgt
     5341 cctggggggc ttcctggctg tagtagggga agggcaggga ccggtcgaag gcccagcggg
     5401 ccgcggcctc tcccgccagg accatcatct cccggaccac ggcgccggag cggtgctccg
     5461 ggatcgcgtc gatgcgcggt tccccggagt ccacccagac ccggacctcc gggatggaga
     5521 tgtccacggc ccccgcggcg gtccgggctt cggagcggat cgaggcgatc cggtccagct
     5581 cggccagggg gccggacccc aggagggggt cggctccgcc gtaggaaagc cgggtgacct
     5641 tcacgagggt gggaaggatc tccgcctccc ggaccgagcc gtccggttcc agggagatcc
     5701 ggacggacaa ggcccggctt acgcccgaca atcccagtcc gaaccgctcc agggccgagt
     5761 ccgggagcat ggggacggaa ccctcgggaa ggtagagggt ggcggcccgg tccgaggcct
     5821 cccggtccgc ggggctcccc gggagcaccg cggaggcggg gtccgccacg tgcacccaaa
     5881 cgtcccggcc gtcgaaggag accgcgtcgt cgggatcccg gctccaggcg ttgtcgatgg
     5941 cccaagcctc aagccccgtc aggtcgaccc ggccgtcgtc gtcctccggt ccgagctcga
     6001 ggtccggtgc cctggacgcg gtcccgaagc ggacgggcca ggggttgacg gcgtcggtcc
     6061 agcggccggt cttgatcagc caggcatgca cggcctcggg cttgtccgcc agtcccaggt
     6121 cacccaggat ccgggccttc tgggtggcac ctagggcgaa ggcctcgacc tcccccagga
     6181 agcgctcgtc cccgggctcg aagaggcctt tccgggcgcg ggccaggaag gcctcccggt
     6241 ccgcggcctc ggtttccttc cgggccctgc gtgcggcctc cttttcccgc tcctcccggg
     6301 aaaccgcccg gaatcccggg tgggcctcct cggtccggaa ccggaggccc tccagggcct
     6361 ccaggcggca ggccagggcc tcctcgggac ccgcccgccc gaagacgagc tcgcagagtg
     6421 cctccaggcc cagggtttcc ccggcgctca tctcccaggc cgtctcgaag tccccgtccg
     6481 ggcgggcctc ggggaccctg gacaggggac ccgggtgcag gagctccacg tccttgggcc
     6541 ggaccttcac ctccggggcg ccgggtatcg agagctccag cttgtccccg gaattccggg
     6601 ccagggcggg ctttcctttg tagaatacga gggctccgtt ggggatcata caaccgcctg
     6661 actggtagat tccgcctgaa gacgggaggg cagtatagca aggaagggga ctcgggagcc
     6721 aggagtcagg acacgggact caggtgccga ccggacaact gcggattccc tcccgtcatc
     6781 agccgccgga aggcccctct gtcgcctggt tcctggcacc tgggtcctgt tacctgtcgc
     6841 ctgactcccg tattgacgaa atgattcttt cgggtatgct taggtgggcg gcgcggaccg
     6901 ccttccggcc ccgccgcagc cccatcggcc ggaccggacg aacatgagga ggaacattcg
     6961 atcatggtag ctctcatagt cggtatcgtc ctgatcctgc tggccgtagt cgcctgcgtc
     7021 cccttcggtc ttgcctggtg gcaggacgtc gtcgtattcc tacggggatc cctccccgtc
     7081 ctcgcggtcc tcatcggcct gatcgcggtc ttcatcggca tcgccgacat caaggaccgg
     7141 atggaggcca agaaggagga ggaagaggaa gccaaggaga acgcggggaa ataaggacac
     7201 aggagtcagg aaccaggagc caggcgcccg atgacagtcc acccgacgga tgcagaagcc
     7261 ggttccgccg aaacctgcgc ggtcgacgga cccctgggtc ctgagtcctg tgacctgagt
     7321 cctgttacct gacttccgtc gccttacttg acacaatccc ccgaatatgg gagtatcacc
     7381 cgcgctattt tccgctgtat tccaagaaca cgaaggaatc gatacatgaa gaccgtcttt
     7441 gtgaagcccg aagaggctcc ccgctcctgg tacctcatcg acgccgaggg caagtccctg
     7501 ggccgcgtgg ccgccaaggt ggcctacatc ctccgcggga agaacaagcc cttctacacg
     7561 ccccaccagg aaatcggcga ctacgtcgtg gtcatcaacg ccgacaaggt ggccgtcacc
     7621 ggcgccaagc gcgaaaacaa gatgtactac catcacaccg ggttccccgg cggcctcaag
     7681 ggccagacct accaggccct ctcggaccgg caccccggca tgcccctgga gatcgccatc
     7741 cgcgggatgc tccccaaggg acccttgggc cggaagctca acaagaacgt caagatctac
     7801 gccggctcca gccacccgca cgccgcgcag cagcccatcg ccctggaact gtaggagaat
     7861 a
//
LOCUS       DAOTEI010000166         7852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6548_length_7852_cov_4.690009, whole genome shotgun sequence.
ACCESSION   DAOTEI010000166 DAOTEI010000000
VERSION     DAOTEI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6548_length_7852_cov_4.690009"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="P5313_13595"
     CDS             complement(<1..408)
                     /locus_tag="P5313_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81448.1"
                     /translation="MSLHFTKRGWAYLALEFAFAWGACGIAWAAGLRSGSPAFLVFAS
                     FFMLTPLATALIVSAARPSGLPLKEVLALRFRMGWKNFFLAWLLFPALALLTLGLSLL
                     LPGVRYDPSMSQLIERMTRGLSPEKIAEIREMAG"
     gene            complement(405..1106)
                     /locus_tag="P5313_13600"
     CDS             complement(405..1106)
                     /locus_tag="P5313_13600"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRY81449.1"
                     /translation="MKTSKFLLAIAILSLAGTAVAYFFLPETIPMHWNFQGEVDSWGP
                     RWSILALGCLPLGVALLMAWLPRIAPRREAYAKHAKTYEILQVILVLMMTGLVWITVA
                     AALGSTLDVSLAVRILIGALFIAMGNYLGRLKRNYFVGIKTPWALADDEVWRLTHRRG
                     ALVFVIMGASFLLSLAFRPGWIVGAVTLVPTILGVAYVFVYSWWVWKKLRGNEKPVIP
                     AASGPEVGKDKEGSS"
     gene            complement(1093..1374)
                     /locus_tag="P5313_13605"
     CDS             complement(1093..1374)
                     /locus_tag="P5313_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HRY81450.1"
                     /translation="MNDVFKALSDPTRREILQMLKRQSLSAGQIASAFDIGKPAISHH
                     LSLLKASDLVQAERDGQNIIYSLNTTVFQDVLAWVYEMRGAKEKTDEDE"
     gene            complement(1508..2587)
                     /locus_tag="P5313_13610"
     CDS             complement(1508..2587)
                     /locus_tag="P5313_13610"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRY81451.1"
                     /translation="MGLESCSVVVNPSRTPHLVRRLLGLLERHGAGRVVVTRDREHFI
                     REIEDFAAGPFRNLIVWGGDGSAHEAQNALVKSGCSGKNVGFLRGGSGNGIQDSYEVP
                     FPIARQVAAFAESAVMDYRIAVDLLAIDDGSGGAPVYGQLCGLGFDVRVLERRARHRS
                     RKTGSARPGLANYVRSGLGLVATEPLSDGRTYRLDLERGKFALRGKRTNAEFPFERLS
                     LDADPVMIEMGTRPYYGGLFKVCPDVVCNDGFLDLYLFNFQNKLDVLRNAVSLWNGWH
                     SRINSRFARRSKPLIERFEIKRVSVSSSEPFLWHVDGEVRLCEPRKDGPARLDIGVLP
                     GALSFLVPAGFWRKFHPYDERRDPS"
     gene            complement(2651..3679)
                     /locus_tag="P5313_13615"
     CDS             complement(2651..3679)
                     /locus_tag="P5313_13615"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HRY81452.1"
                     /translation="MNRLDRVLSLLQLLRQGRPVSASVLARRFGVSVRTVYRDLEALA
                     GLGLPVEPEMGRRGGFRLGPGYFLPPVTLNPEEAVSLLLGLSLGRALRVLPFPEGTDE
                     AERKLLAVLPEETRRILERASRFIGFEKVPPDLLHPERGAPQSVGQAVEEESRIVGRF
                     LRAVLSRSRLRISYRSPYRDGTEPLREIEPRGILWDRDRWYLVGDVEVRSGEPRLWRS
                     DRVVEMRPGPAIRPTSSEFDVSDLLGRRWLGKAMERWRAEHPVRIALGEAQAEALRRD
                     WYYGHALFEEGPDGRTVLTVGEGEYERIAAFLRWLGPGAELLEPAGWRDRFAGELEAM
                     AEAYRRPG"
     gene            3751..4695
                     /locus_tag="P5313_13620"
     CDS             3751..4695
                     /locus_tag="P5313_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY81453.1"
                     /translation="MNSRAAYAAGIAWSTIFGLSFLVTKSALEAFSPFTLLALRFGLA
                     TAALLALSAAGILKPDFRGKPRGILVLVCLFQPILYFTSETFGVRETSTSTAGLILGA
                     LPAAVAALSVPMLRERLSGARTAGLALSVAGVSLVAAAGGGGGADEPGGVLLVLGALA
                     SAAFYNVFSRRASRFYGPVEITLAMMASGAGFFGLAALAERLAVGPAAPGIRPGAEAW
                     AAVAYLGILSSVLAFFLVNLSLSRLRAAQASVFGALTPLIALAAGVLVRGEPVSLPKA
                     AGAAAILAGLWITNAPERTAPASAGVGAAATNTERTGA"
     gene            4735..5256
                     /locus_tag="P5313_13625"
     CDS             4735..5256
                     /locus_tag="P5313_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81454.1"
                     /translation="MDGATDRTIIGREAWTRALLALFDEGFRGARGAGTWYVDNDPEC
                     GLLGTVAGLSAGEASRPLAPGDPLTAASHVDHVRFALSLANRASRGENPYPTADWASS
                     WAVRSVTDGEWADLVASLESEIADLRRYIEGGKPLEDEMFATGIFGLVAHTAWHLGAL
                     RQALGFIRAPAES"
     gene            complement(5302..6093)
                     /locus_tag="P5313_13630"
     CDS             complement(5302..6093)
                     /locus_tag="P5313_13630"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HRY81455.1"
                     /translation="MGPLSLLKTARRRRILERSPIPEELWDWALKEHRIFRSLDPEDS
                     ARLRELSTIFLAEKEFRPLPGAVLDDRTRVSIAAQACLPLVGLDIDWYSDWTTILVTP
                     REYEVTRRDVDEAGVVTEYDDELAGEVLHLGPVVLSLEDVEDSGWGDGFNVVIHEMAH
                     KLDGRDGSFDGCPPLRRDMDGEAWRLAFTEAYEDLRAKVARAPRRRSRRRGPRIDPYA
                     AESPDEFFAVACEYFFEKPILLKSEYPEVYGQLVRFFRRDPLSRD"
     gene            complement(6098..6727)
                     /locus_tag="P5313_13635"
     CDS             complement(6098..6727)
                     /locus_tag="P5313_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81456.1"
                     /translation="MNLNRPELTTQALSILRNPETFQWYFIPLLAFTVYVYFNEIQKK
                     NWKGVAAGLSLYMVHWLYEIANAMIQHFTGHALWTVPGGTSFLLLVGVGWELSLMFAV
                     AGLIFSKILPEDPKLKLFGINNRILFAVLNAGFFSVFEIFLAATPAFHWVYPWWGAFP
                     VFVTVYIPFFLAANLCHDWPPVRQRRFIGSLALVNAVLLLVFAGMLGWI"
     gene            6864..7496
                     /locus_tag="P5313_13640"
     CDS             6864..7496
                     /locus_tag="P5313_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRY81457.1"
                     /translation="MDESSFILGVDLDGVVGDFYTSMRQIAAEWLDRPVESLATDVSF
                     GLSEWGLDEFGGYEKLHRFAVTQRDLFRILPPVKGASATLRKLSERGIRIRIITHRLF
                     LKYFHKEAIAQTIDWLDAHAIPYWDLCFMKDKGAVGAHVYIDDSPSNVENLRAQGCRT
                     IVFGNSTNRHLPPPRAESWSEVERLVMEAHEEWRTGTLGLFGAADYIQRP"
     gene            complement(7637..>7852)
                     /locus_tag="P5313_13645"
     CDS             complement(7637..>7852)
                     /locus_tag="P5313_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81458.1"
                     /translation="VVDNPDFNLVKIFLCNLYDWLSSRHRFSGHYVRTAYPRVREILD
                     RRGCPASARFLEFLLAGGARRAGASRD"
BASE COUNT         1307 a         2600 c         2761 g         1184 t
ORIGIN      
        1 cccggccatc tcccggatct ccgcgatctt ctcggggctc aacccccggg tcatccgctc
       61 gatcagctgg gacatggaag gatcgtagcg gactccgggc aggaggaggg aaaggcccag
      121 ggtcagcaag gccagggcag ggaagaggag ccaggccagg aaaaaattct tccagcccat
      181 ccggaaccgc agggccagga cctccttcag ggggaggccg gaaggccgcg ccgccgatac
      241 gatcaacgcc gtggcgaggg gggtcagcat gaagaaggac gcgaacacca ggaacgccgg
      301 ggagcccgag cggaggcccg cggcccaggc tatgccgcag gctccccacg cgaacgcgaa
      361 ctccagggct aggtaggccc agccccgctt cgtgaagtgc agactcatga ggatccctcc
      421 ttgtccttgc cgacttcggg cccggaggcc gccgggatga cgggcttctc gttcccccgc
      481 agctttttcc agacccacca ggagtacacg aacacgtacg cgacccccag gatggtcgga
      541 accagggtca cggctcccac gatccaaccc ggccggaagg ccagggagag caggaaggaa
      601 gcgcccatga tcacgaagac cagggctccc cggcggtggg tgaggcgcca gacctcgtcg
      661 tccgccaggg cccagggggt cttgatgccc acgaagtagt tgcgcttgag gcggcccagg
      721 tagttcccca tcgcgatgaa gagggccccg atcaggatgc ggacggccag gctgacgtcc
      781 agcgtcgagc ccagggccgc ggccacggtg atccagacca gccccgtcat catgaggacg
      841 aggatcacct ggaggatctc gtaggtcttc gcgtgcttgg cgtaggcttc ccgccggggg
      901 gcgatccggg ggagccaggc catcaggagg gcgacgccca ggggcaggca gcccagggcc
      961 aggatgctcc accgcggccc ccaggagtcc acctcgccct ggaagttcca gtgcatgggg
     1021 atggtttccg gcaggaagaa ataggcgacg gcggtcccgg ccagggacag gatcgcgatg
     1081 gccagcagga atttactcgt cttcatcggt tttctccttg gctccccgca tctcgtatac
     1141 ccaggcgagc acgtcctgga acacggtggt gttgagggaa tagatgatgt tctgcccgtc
     1201 ccgctccgcc tgcaccaggt ccgaagcctt taaaaggctc aggtggtggc tgatggccgg
     1261 cttgccgatg tcgaaggcgg aggctatctg ccccgcgctc aggctttgcc gcttcagcat
     1321 ctggaggatc tccctccggg tcggatcgga gagggccttg aaaacgtcgt tcactacggc
     1381 gcctcctatt gttcgacaat tcgaataata tcgaaatatc gaaataaggt caagcggaga
     1441 cgtgaatccc aggcgggcgc gagggcaggg aaaccggtgg acggagggac ggaaagccga
     1501 cggggaatca ggagggatcg cggcgttcgt cgtaggggtg gaacttgcgc cagaaccccg
     1561 cgggcaccag gaagctcaag gcccccggga ggacgccgat gtccagccgg gccggcccgt
     1621 ccttccgggg ctcgcagagc cgtacctccc cgtccacgtg ccacaggaag ggttccgacg
     1681 aggagacgga taccctcttg atctcgaaac gctcgatgag gggcttggat ctccgggcga
     1741 agcgggaatt gatccgggag tgccagccgt tccacaggga gaccgcgttg cgcaggacgt
     1801 ccagcttgtt ctggaaattg aagaggtaga ggtccaagaa gccgtcgttg cagaccacgt
     1861 cggggcagac cttgaacagg cccccgtagt acggccgggt ccccatctcg atcatcacgg
     1921 gatccgcgtc cagggaaagc cgctcgaagg ggaactccgc gttggtgcgc ttgccgcgca
     1981 gggcgaactt gccgcgctcc aggtccaggc ggtaggtccg gccgtccgag agaggttccg
     2041 tggccacgag cccgagcccc gagcggacgt agttggccag gcccgggcgg gccgacccgg
     2101 tcttccggga ccggtgccgc gcccggcgct ccaggacccg gacgtcgaag cccaggccgc
     2161 agagttggcc gtacacgggg gcgcccccgc ttccgtcgtc gatggccagg aggtccacgg
     2221 cgatgcggta gtccatgacc gcgctctccg cgaaggcggc aacctgccgg gcgatcggga
     2281 agggcacctc gtaggagtcc tggatcccgt tcccggagcc tccccggagg aaccccacgt
     2341 tcttgccgga gcagccggac ttcaccaggg cgttctgggc ctcgtgggcc gatccgtccc
     2401 cgccccagac gatgaggttc cggaaggggc ccgcggcgaa gtcctcgatc tcccggatga
     2461 agtgttcccg gtcccgggtg acgacgaccc ggccggcccc gtgcctttcc aggagcccga
     2521 ggaggcgccg gaccaggtgg ggcgtccggc tgggattcac gacgacggaa caggattcca
     2581 ggcccatggc ggggagtata tctctctcgg gagcggcagg ccaggggcgt tccggggaaa
     2641 tcccgaggca tcagccgggc cggcggtagg cctccgccat ggcctccagt tccccggcga
     2701 agcggtcgcg ccagccggcg ggctccagga gttcggcccc gggccccagc cagcgcagga
     2761 aggcagcgat ccgctcgtac tccccctccc ccacggtcag gacggtccgg ccgtccgggc
     2821 cctcctcgaa gagggcatgg ccgtagtacc agtcccgtcg cagcgcctcc gcctgggcct
     2881 cccccagggc tatccgcacc gggtgttccg cccgccaccg ttccatggcc tttccgagcc
     2941 accgccgtcc cagcaggtcg gacacgtcga actccgagga ggtgggccgg atggccggcc
     3001 cgggcctcat ctcgacgacc cggtcggacc gccacaggcg gggttccccg gaccggactt
     3061 cgacgtcccc cacgaggtac caccggtccc ggtcccacag gatgccccgg ggctcgatct
     3121 cccgcagggg ttccgtcccg tcccggtagg gggaccggta ggagatccgg agccgggacc
     3181 gggacaggac ggcccggagg aagcggccca cgatccggga ctcctcctcc acggcctggc
     3241 cgacggactg cggcgcgccc cgctcgggat gcaggaggtc cggcgggacc ttctcgaagc
     3301 cgatgaaccg ggacgcccgc tccaggatcc gtcgggtttc ctcggggagg accgcgagca
     3361 gtttgcgctc ggcctcgtcc gtcccctcgg ggaaggggag gacccggaga gcccggccga
     3421 gggacaggcc caggagcagg ctgaccgcct cctcggggtt gagggtgaca gggggtagga
     3481 agtaccccgg accgaggcga aagccgcccc gcctgcccat ctccggttcg acgggaaggc
     3541 ccagtccggc cagggcctcc aggtcccggt agaccgtgcg gacggatacc ccgaaccgcc
     3601 gggccaggac ggaggcggac acgggacgcc cctgccggag gagctggagg agggacagga
     3661 cgcggtccag gcggttcatg attcccgaaa agtatgacat atcctgacac ggtcggatag
     3721 tatgttcacc tccgagtcgg gaggttttcc atgaacagtc gagcggccta tgccgccggt
     3781 atcgcctggt ccacgatatt cggcctttcc ttcctggtga ccaagtcggc cctggaggcc
     3841 ttctcgccct tcaccctgct ggccctgcgc ttcgggctgg cgaccgcggc cctgctggcc
     3901 ctgtccgcgg ccgggatcct gaaacccgac ttccggggca agccccgggg catcctggtc
     3961 ctggtgtgcc tcttccagcc gatcctgtac ttcacctccg agaccttcgg ggtccgggag
     4021 acctcgacga gcaccgcggg cctgatcctg ggggccctgc ccgccgcggt ggcggccctt
     4081 tccgtgccga tgctccggga gaggctctcc ggggcccgga ccgccgggct ggccctgtcc
     4141 gtggcggggg tctccctggt ggccgcggcc ggggggggag gaggggccga cgaaccgggc
     4201 ggggtcctcc tggtcctggg cgccctggcc agcgccgcct tctacaacgt gttttcccgc
     4261 cgggccagcc ggttctacgg ccccgtggag atcaccctgg cgatgatggc ctccggggcc
     4321 ggcttcttcg gcctggcggc cctggccgag cggctggcgg tcggtcccgc ggccccgggc
     4381 atccggcccg gcgccgaggc gtgggccgcg gtcgcgtacc tggggatcct gtcctcggtg
     4441 ctcgccttct tcctggtcaa cctgagcctg tcgcggctcc gggccgccca ggcctcggtc
     4501 ttcggggccc tcacgcccct gatcgccctg gccgcggggg tgctcgtccg cggggagccc
     4561 gtgagccttc cgaaggcggc cggggccgcg gcgatcctgg cggggctctg gatcacgaac
     4621 gccccggagc ggacggcgcc ggcctcggcc ggggtcggcg cggcggctac gaatacggag
     4681 cggacggggg cctagccccc ggaaccgtaa gacagggaac ggggaggtgc gagaatggac
     4741 ggagcgacgg accgaacgat catcgggcgc gaggcttgga cgagggccct cctggccctg
     4801 ttcgacgagg ggttccgggg ggcccgggga gccggaacct ggtacgtgga caacgacccg
     4861 gagtgcggcc tcctgggaac cgtcgcgggc ctctccgccg gggaggcctc ccggcccctg
     4921 gccccggggg atcccctgac ggccgcctcc catgtcgacc acgtgcgctt cgccctgtcc
     4981 ctggccaacc gggcctcccg gggcgagaat ccctacccga ccgcggactg ggcctccagc
     5041 tgggccgtcc ggtccgtgac ggacggggaa tgggcggact tggtggcctc cctggagtcc
     5101 gagatcgcgg acctgcgtcg gtacatcgag ggcgggaagc ctctggagga cgagatgttc
     5161 gccacgggga tcttcggcct cgtggcccac acggcctggc acctgggagc cctgcgccag
     5221 gccctggggt tcatacgggc gccggccgaa tcctgacgga aggaggccgg accttacgct
     5281 gacggcctag gaccgcacga ttcaatcccg ggacagcggg tccctgcgga agaaccggac
     5341 gagctggccg tagacctccg ggtattcgga cttcaggagg atcggcttct cgaagaagta
     5401 ctcgcaggcc acggcgaaga actcgtccgg ggactccgcg gcataggggt cgatgcgcgg
     5461 tccccgcctc cgggatctcc ggcggggtgc ccgggcgacc ttggctcgca ggtcctcgta
     5521 ggcctccgtg aaggcgaggc gccaggcctc gccgtccatg tcccgccgca ggggcggaca
     5581 gccgtcgaag gaaccgtccc ggccgtccag cttgtgggcc atctcgtgga tcaccacgtt
     5641 gaaaccgtcc ccccagcccg agtcctccac gtcctcgagg gacaggacca cggggcccag
     5701 gtggagtacc tcccccgcca gctcgtcgtc gtactccgtg accaccccgg cctcgtccac
     5761 gtcccgccgg gtgacctcgt actcccgggg ggtgaccagg atggtggtcc agtcggaata
     5821 ccagtcgatg tccagtccga ccaggggcag gcaggcctgg gcggctatgg aaacccgggt
     5881 gcggtcgtcc agcaccgcgc ccggcagggg gcggaattcc ttttccgcca ggaagatcgt
     5941 ggagagctcc cggaggcggg cggagtcttc cgggtccaga ctccggaaga tccggtgttc
     6001 cttgagcgcc cagtcccaga gttcctccgg gatcggggac cgctccagga tccggcgtcg
     6061 ccgggccgtc ttcaggaggg agagggggcc catcgcccta gatccacccc agcatgcccg
     6121 cgaagaccag gagcaggacc gcgttcacca gggccaggga tccgatgaac cgccgctgcc
     6181 tgaccggggg ccagtcgtgg cagaggttgg cggccaggaa gaaggggatg tagacggtga
     6241 cgaagaccgg gaaggccccc caccaggggt aaacccaatg gaaggcgggg gtcgcggcca
     6301 ggaagatttc gaagaccgag aagaagcccg cgttcaggac cgcgaagagg atccggttgt
     6361 tgatcccgaa aagcttgagc ttcgggtcct cgggcaggat cttggagaag atgagccccg
     6421 ccacggcgaa catcagggag agttcccagc cgactccgac caggagcagg aaggaggtcc
     6481 cgcccgggac ggtccagagt gcgtgacccg tgaagtgctg gatcatggcg ttggcgatct
     6541 cgtagagcca gtggaccatg tagagcgaga gccccgcggc cactcccttc cagttttttt
     6601 tctggatctc gttgaagtag acgtagaccg tgaaggccag caagggaatg aaataccact
     6661 ggaaggtctc ggggttccgg aggatcgaga gcgcctgggt ggtcagttcc ggccggttca
     6721 ggttcatggg agcctcctga tggaagccca tggtagcatt ccgcgcctcc ttcggcgagt
     6781 gcgcgtcgaa gtttagctta ggcgattttg gtggggccaa ggaggagtgg acggacggcg
     6841 gcgggcgcgc catactggcg ggtatggacg agagcagttt catcctgggc gtggacctgg
     6901 acggggtggt gggggacttc tacaccagca tgcggcagat cgcggcggag tggctggacc
     6961 ggccggtcga gtccctggcc acagacgtgt ccttcggcct ttccgagtgg gggctggacg
     7021 agttcggggg ctacgagaag ctccaccgtt tcgccgtgac ccagcgggac ctcttccgga
     7081 tcctgccccc cgtgaagggc gcctcggcca ccctgcgcaa gctgtccgag cgtgggatcc
     7141 gcatccgcat catcacccac cggctcttcc tgaaatactt ccacaaggaa gcaatagccc
     7201 agaccatcga ctggctggac gcccacgcca tcccctactg ggacctctgc ttcatgaagg
     7261 acaagggggc cgtgggagcc cacgtctaca tcgacgactc accctccaac gtggagaacc
     7321 tccgggccca gggctgccgt accatcgtgt tcgggaattc cacgaaccgc cacctgccgc
     7381 ccccccgggc ggaatcctgg tccgaggtgg agcgcctggt gatggaggcc cacgaggagt
     7441 ggcggaccgg caccctgggg ctcttcggcg cagcggacta tatccagcgt ccctagggag
     7501 cctgtcggac ttgtggattt ttagcgccga taggcgctaa aaatcccgat tatacggctc
     7561 ctttggagtt tgcaaggtag aaaaatcgca caaatgcgat ttttttacta tttaggtgga
     7621 caagcccgac aaactcctag tcccgggacg cccccgcgcg ccgcgctccc ccggccagga
     7681 ggaactccag gaaccgggcc gaggcggggc agccccggcg gtccaggatc tcccggaccc
     7741 ggggataggc cgtccgcacg tagtgacccg agaaccggtg ccgggacgag agccagtcgt
     7801 agaggttgca caggaagatc ttcacgaggt tgaagtccgg gttgtctact ac
//
LOCUS       DAOTEI010000167         7847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6555_length_7847_cov_4.645149, whole genome shotgun sequence.
ACCESSION   DAOTEI010000167 DAOTEI010000000
VERSION     DAOTEI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6555_length_7847_cov_4.645149"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="P5313_13650"
     CDS             complement(<1..155)
                     /locus_tag="P5313_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006073620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HRY81459.1"
                     /translation="MRVSVVTVSDRASRGEYEDLSGPEIERILRETVPGAEVERRVVP
                     DEEEAILG"
     gene            complement(124..573)
                     /locus_tag="P5313_13655"
     CDS             complement(124..573)
                     /locus_tag="P5313_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRY81460.1"
                     /translation="MERAGEFRVLSINVSRETGVQKEPVDFAVLKPAHGIEGDAHAGD
                     WHRQVSLLADEDIETMRGKGVDLDFGDFAENITTRGVDLGSLPVGTRLRIGEAELEVT
                     QIGKECHHGCAIYQAVGDCVMPRKGIFARVLKGGRIDRESVGRYRIG"
     gene            complement(573..1034)
                     /gene="moaC"
                     /locus_tag="P5313_13660"
     CDS             complement(573..1034)
                     /gene="moaC"
                     /locus_tag="P5313_13660"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRY81461.1"
                     /translation="MAESDRLTHVDGSGQARMVDVSGKPRVRRTARAEGFVFLAPGTV
                     RLVRENALAKGDVLAAARLAGILAAKRTSDLIPLCHNIEIEHVEVRLEVLDDRIRIEA
                     SAACTDKTGIEMEALTAVSVAALTVYDMCKAVDKGMRIEGVRLLEKTKEAL"
     gene            complement(1036..1833)
                     /locus_tag="P5313_13665"
     CDS             complement(1036..1833)
                     /locus_tag="P5313_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY81462.1"
                     /translation="MLDSFDRTIDYLRVSVTDRCNLRCVYCMPAEGVPLLRHEDILSL
                     EEIVRIAEAAVGLGIRKIRLTGGEPLVRKGILDLVSLLRSLPGLEILGMTTNGTLLAP
                     VAEELRARGLDSVNVSLDTLDPGRFSALTRGGRLADALAGIDAAVRAGIPVKINTVAF
                     DGDPEGDLGRVADFCAERGLRHQRIRRYDLAGQKFDQDDYERPPRCGDCNRIRLLADG
                     SLKPCLHSDLSVPVDFSDIEGSLRACVRAKPGRGAACTTLAVGQIGG"
     gene            complement(1817..2989)
                     /locus_tag="P5313_13670"
     CDS             complement(1817..2989)
                     /locus_tag="P5313_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRY81463.1"
                     /translation="MTHPDEALALVARQEVSILPEEPEAVPLSRALGRVLARDLPATV
                     DQPPFDKSAMDGFAWGGTLSDSFRVLGTLAAGRVADHPLAPGDCVRIMTGAPLPPGAD
                     RVQRVEWTEEADGRVRFTRRESSDNVIRRGENQKSGDLLLGRRILRPADIGILASSGY
                     ESVPVQRRPVVGILSTGDELRTPGEPLVPGAIYDSNGPQLAAQAAAEGCEVRSYGRFP
                     DLPVALETAVKAALSECDLVLLSGGVSMGDFDYVPEVLARNGVERIFHNLAMKPGKPT
                     YLGRRENVFVLGLPGNPVSTFVNFEILAKAVLARLRGLPWEPAIASARLAEEVSRRDS
                     ERVEFLPARLSGGEIRALRYSGSSMLGVLAEANALLRLEIGQKILERGAQIDARLL"
     gene            complement(2986..3699)
                     /locus_tag="P5313_13675"
     CDS             complement(2986..3699)
                     /locus_tag="P5313_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HRY81464.1"
                     /translation="MRSEAILSVRDLGFSYGTREILSIPELDVRAGETLALVGPNGSG
                     KTTLLKLLNDLIRPSSGSILFEGRPAAGDPGLRLRSVYVHQSPFLLAGTVYRNVAYGL
                     RVRRVPEPEIRRRVEASLDLLGLAGFKRRDAKRLSGGETQRIALARALALEPELLLLD
                     EPTAQTDRASGERIRTVLADLATRGGTTIVFSSHDAAFAEALADRAAFLEEGRLVRIE
                     PGRRRYAADLPSQAPESPV"
     gene            complement(3689..4369)
                     /locus_tag="P5313_13680"
     CDS             complement(3689..4369)
                     /locus_tag="P5313_13680"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81465.1"
                     /translation="MNPFLGALELIVSGDREVFLIAGTTLHFSFWSTAFSTLPGLALG
                     ILLANYRFPGRRAVAAVVSSLTALPTVVIGLFVYSFISRSGPLGSLGWLFAPPGVVLG
                     QTLLALPIVVSLVYTGLSKLDARFTETLVTLGAGRLRRLASTLFEAKYVLASALLNAF
                     GRVTGEVGVSMMLGGNIRWYTRTMTTTIALDTSKGEFERALSLGILLLTISLTINALV
                     HAAVKHEE"
     gene            complement(4370..5134)
                     /locus_tag="P5313_13685"
     CDS             complement(4370..5134)
                     /locus_tag="P5313_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY81466.1"
                     /translation="MGGKEAAAPANPRIKLATTTSTENSGLLGYILPIFEAKTGYKVD
                     VVAVGTGAALKLGENGDADVVLVHARAQEDAFVAAGHGVDRRDVMYNDFIILGPASDP
                     AGIGSAKSSTEAFSRIASAGATFVSRGDKSGTHVKELDIWKAAGIAPAGTWYKEAGQG
                     MEQVILMANGMPGYTLADRGTWLAVKDKTSLKICYQGDKGLFNPYGIIAVNPAKWPSA
                     NNEGAKALIAWIMSKEGLKAIESFKLNGEQLFFTYK"
     regulatory      complement(5277..5456)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            5731..7554
                     /locus_tag="P5313_13690"
     CDS             5731..7554
                     /locus_tag="P5313_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY81467.1"
                     /translation="METVTFESLGLEPEILEALDRKGFEEPTPIQALAIPRLLSGGAN
                     LVARARTGTGKTAAFGLPLIQNLRVPTGAVRALVLVPTRELALQVSGEVSSLRPHGDY
                     PRVAPIYGGASYGEQFRRLSRGVEIVVGTPGRVKDHIERGSLSLEALEYFVLDEADEM
                     LDMGFLEDIEWIFEKAPADRRVLLFSATMPKEILRIAKTRLGEYEIVADDSGPVETAL
                     TDQIWIEVREADKLEVLCRLVDKEDEFYGIVFCATKVDTDALAKSLGERGYGAEALHG
                     DLSQVEREKVLRRFRTKRTTILTATDVAARGIDIDRLTHVVNFSLPHDPDSYTHRIGR
                     TGRAGNTGTAVTLVTPDEYRRIFFLRKTAGAALKKGAVPEVDEVLEAKRQRIRGRVLS
                     AAADLGILSEETEGSAGQAPGEAGDASAEPGKTGEKAGTPQHSEEARERWRILAREIS
                     EGLDPLDALAAALAAGYSSELDPGRYGDVRTDFLDKEATTRLFIGAGRRDGADRKAIA
                     VLVKDLAGLPDRLVGGVEVYDAFSFVTVPFEAAERIIAEARKSGKGPVVKMAFPKDGA
                     PARRGPPAYRKFNPGDRGPRDRDGFSPPGRTRGRRTNTRSG"
     gene            complement(7502..>7847)
                     /locus_tag="P5313_13695"
     CDS             complement(7502..>7847)
                     /locus_tag="P5313_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81468.1"
                     /translation="KRTGTLDSRGPETVLPPRDRLEEAMKRAASSLSPARTGLDEGGE
                     RAALTRALAAEEETSAFYRSLVAALPEESRAVFHRFVEIEDGHTSIVRAQLDLVSRTG
                     YWFDVREFDLED"
BASE COUNT         1235 a         2678 c         2744 g         1190 t
ORIGIN      
        1 cccaggatgg cctcctcctc gtcggggacc acccggcgct cgacctcggc gccgggcacc
       61 gtttcccgca ggatccgctc gatctcggga cccgagaggt cctcgtactc cccccggctg
      121 gccctatccg atacggtaac gaccgacact ctcacggtct atcctccccc ccttgagcac
      181 ccgggcgaat atgcccttcc ggggcatgac gcagtccccc acggcctggt agatcgcgca
      241 gccgtggtgg cattccttcc cgatctgggt gacctccagc tccgcctcgc cgatccggag
      301 gcgggtcccc acgggcaggg atccgaggtc cacgccccgg gtggtgatgt tctccgcgaa
      361 gtccccgaaa tccaggtcca cgcccttgcc ccgcatggtc tcgatgtcct cgtccgccag
      421 gagggagacc tggcggtgcc agtccccggc gtgggcgtcc ccctcgatcc cgtgggcggg
      481 cttcaggacc gcgaagtcca cgggctcctt ctggaccccc gtctcccggg agacgttgat
      541 cgacaggacc cggaattccc cggcgcgttc catcacaggg cctccttcgt cttctccagg
      601 aggcgcaccc cctcgatccg catccccttg tccacggcct tgcacatgtc gtagaccgtc
      661 agggccgcca ccgatacggc ggtcagggcc tccatctcga tgccggtctt gtccgtacag
      721 gccgccgagg cctcgatgcg gatccggtcg tccaggacct ccaggcggac ctcgacgtgc
      781 tcgatctcga tgttgtggca cagggggatg aggtccgagg tgcgcttggc cgccaggatc
      841 cccgccagac gggccgccgc caggacgtcc cccttggcca gggcgttctc ccggaccagc
      901 ctcacggtcc cgggggccag gaacacgaag ccttccgccc gggccgtgcg ccgtacccgg
      961 ggcttgcccg agacgtccac catccgtgcc tgtcccgacc cgtccacgtg ggtcagccga
     1021 tccgattccg ccatcctatc ctccgatctg gcccacggcc agggtcgtgc aggccgcgcc
     1081 ccggcccggc tttgcccgca cgcacgcgcg caggcttccc tcgatgtccg agaaatccac
     1141 gggaacggag aggtccgagt gcaggcaggg cttaagggat ccgtccgcca ggagccggat
     1201 ccggttgcag tccccacagc gggggggacg ctcgtagtcg tcctggtcga acttctgtcc
     1261 cgccaggtcg tagcgccgga tgcgctggtg ccgcagtccc cgttccgcgc agaagtcggc
     1321 cacccgcccc aggtcgccct cggggtcccc gtcgaacgcc acggtgttga tcttgactgg
     1381 gatgcccgcc cggaccgcgg cgtcgatccc cgcgagggcg tccgcgaggc ggccgccccg
     1441 ggtcagggcc gagaagcggc cgggatccag ggtgtccagg gagacgttaa cggaatccag
     1501 gccccgggcg cggagctcct cggccacggg agccaggagg gtcccgttgg tggtcatccc
     1561 caggatctcc aagcccggaa gggaccgcag gagggagacc aggtccagga tgcccttgcg
     1621 gaccaggggt tcccccccgg tcaggcggat cttccggatc cccaggccta cggccgcctc
     1681 cgcgatccgg acgatctcct cgaggctcag gatgtcctcg tgccggagga ggggcacccc
     1741 ctcggcgggc atgcagtaca cgcagcgcag gttgcatcgg tccgtgaccg agacccgcag
     1801 gtagtcgatg gtgcggtcaa aggagtcgag catcgatctg ggctccccgt tccaggattt
     1861 tctgtccgat ttccagccgc aggagggcgt tcgcttcggc caggacgccc agcatggagg
     1921 accccgagta ccggagggcc cggatctccc ccccggaaag ccgggcgggc aggaactcga
     1981 cccgttcgga gtcccggcgg gagacttcct cggccaggcg ggcggacgcg atcgcgggtt
     2041 cccagggcag tccgcggagt cgggccagca cggccttggc caggatctcg aagttcacga
     2101 aggtggagac cggattcccg ggaaggccca ggacgaagac gttctcccgc cttcccaggt
     2161 acgtgggttt cccgggcttc atggccaggt tgtggaagat cctctccacg ccgttccggg
     2221 ccaggacctc gggaacgtag tcgaagtccc ccatggatac ccccccggac aggaggacca
     2281 ggtcgcattc cgacagggcc gctttcacgg cggtctccag ggccaccggg aggtccggga
     2341 agcggccgta ggagcggacc tcgcagccct ccgccgcggc ctgggccgcc agctggggtc
     2401 cgttggagtc gtagatggcc cccggcacca gaggctcgcc gggggtccgg agctcgtccc
     2461 ccgtggagag gatccccacg acgggcctgc gttgaacggg gacggactcg tacccggagg
     2521 acgccaggat gccgatatcc gcgggccgaa ggatacgtcg ccccaggagg aggtccccgg
     2581 acttctggtt ctctccacgc cggatgacgt tgtccgagga ttcccggcgg gtgaaccgga
     2641 cccgaccgtc cgcctcctcg gtccactcca cccgctggac cctgtccgcc ccgggcggaa
     2701 ggggggctcc ggtcatgatc cggacgcagt ccccgggggc gaggggatgg tccgccaccc
     2761 ggccggcggc gagggtcccg aggacccgga aggaatccga gagggttccg ccccaggcga
     2821 atccgtccat ggcggacttg tcgaaggggg gctggtcgac cgtcgcggga aggtcccggg
     2881 cgaggaccct ccccagggcc cgtgacaagg gtaccgcctc cggttcctcg ggcaggatgg
     2941 agacttcctg ccgggcgacg agggccaggg cttcgtcggg gtgcgtcata cgggagactc
     3001 cggagcttgg gatggtaaat cggccgcgta ccgccgccgg ccgggttcga tgcgcacgag
     3061 gcggccctcc tccaggaagg ccgcccgatc ggccagggct tccgcgaagg ccgcatcgtg
     3121 ggaggaaaag acgatggttg tcccgccccg ggtcgcgaga tccgccagga ccgtccggat
     3181 gcgctccccc gaggcccggt ccgtctgggc cgtgggctcg tccaggagga gcagttccgg
     3241 ttccagggcc agggcccggg ctagggcgat ccgctgggtc tcgcccccgg acaagcgctt
     3301 ggcgtccctg cgcttgaaac cggccaagcc caggaggtcc agggatgcct ccacccggcg
     3361 gcggatctcc ggttccggta cccggcgcac ccgaaggccg taggccacgt tccggtacac
     3421 ggtgcccgcc aggaggaagg gggactggtg gacgtagacg gaccggaggc ggagtcccgg
     3481 atcccccgcc gcgggcctgc cctcgaagag gatcgagccc gaggagggcc ggatcaggtc
     3541 gttcaggagc ttgaggaggg tggtcttccc ggacccgttc ggtcccacca gggccagggt
     3601 ttccccggcc cgcacgtcca gctccgggat cgagaggatc tcccgggttc cgtaggagaa
     3661 cccgaggtcg cggaccgaga ggatcgcctc actcctcatg cttcaccgcc gcgtgcacca
     3721 gggcgttgat cgtgagtgag atggtcaaaa ggaggatgcc cagggagagg gcgcgctcga
     3781 attcgccctt gctcgtgtcc agggctatcg tcgtggtcat ggtacgggtg taccaccgga
     3841 tgttcccccc cagcatcatg gacactccga cctccccggt gacccgtccg aaggcgttca
     3901 gcagggcgga ggccaggacg tacttggcct cgaagagggt ggaggccaag cgtcggagac
     3961 ggccggctcc cagggtgacc agggtttccg tgaaccgggc gtccagcttg gagagccccg
     4021 tatagaccag actgaccacg atcggcaggg ccaggagggt ctggccgaga acgacccccg
     4081 ggggggcgaa gagccatccc aggctcccca ggggaccgga ccgggagatg aaggagtaca
     4141 cgaaaaggcc gatcaccacg gtgggcaggg ccgtcaggct cgacaccacc gcggctacgg
     4201 ccctgcggcc cgggaaccgg tagttcgcca ggaggattcc cagggccagc cccggaagcg
     4261 tggagaaggc ggtggaccag aaggagaagt gcaacgtcgt gcccgcgatc aggaagacct
     4321 ctcggtctcc cgaaacgatc aactccaggg ctcccaggaa cggattcatc tacttgtacg
     4381 tgaagaagag ctgctcgccg ttcagtttga aggattcgat ggccttgaga ccttccttgc
     4441 tcatgatcca ggcgatgagg gccttggcgc cctcgttgtt ggcggagggc cacttggccg
     4501 ggttgacggc gatgatcccg tagggattga acaggccctt gtcgccctgg tagcagatct
     4561 tgagggacgt cttgtccttg accgccagcc aggtaccccg gtccgctagg gtatacccgg
     4621 gcatgccgtt ggccatgagg atgacctgct ccatgccctg gccggcttcc ttgtaccagg
     4681 ttcccgcggg ggcgatcccg gcggccttcc agatgtccag ctccttcacg tgggttccgg
     4741 acttgtcccc ccgggacacg aaggtggcgc cggcggaggc gatcctggag aacgcctcgg
     4801 tagaggactt cgcggagccg atgcccgcgg gatccgaggc ggggcccagg atgatgaagt
     4861 cgttgtacat gacgtcccgg cggtccactc cgtgaccggc ggccacgaag gcgtcctcct
     4921 gggcccgggc gtgtaccagg accacgtcgg cgtccccgtt ctccccgagt ttgagggccg
     4981 ccccggtgcc cacggccacg acgtcgacct tgtagcctgt cttggcctcg aagatgggca
     5041 ggatgtaccc caggaggccg gagttctcgg tgctggtggt ggtggccaac ttgatgcggg
     5101 gattggccgg ggcggcggct tccttgccgc ccatcgcgaa ggcggaagcc gcgcagaggg
     5161 cggccagggc gaggaccagg gcttttcggt tcacgggttg cctcctcgaa gaacgatgtt
     5221 gtccgagagg cccggaagag ggatatccgg tgcttttctg taaggaatca ggagatggag
     5281 catggggaag ttctcctttc caaacgggag gctcgggaat ttctccccgg accctcggcc
     5341 gggcggtcgg cgcgccccgg gtcgggaacg cggatccagc cgtccggcac cgcccgctcg
     5401 ccctggatcc cggtacggga aggttaggcg aagacgggtc ggagacgttg tccgcccacg
     5461 gcggacgggg ccagaataaa acaggaccgt cggattgtca acgaaacccg ggacgccggg
     5521 agggtttcga tccggcccgg gagcgagaac ttcccgctag acccccgcgg cccggttccg
     5581 gtaagatagg atattccgtg atcgcccgcc gacgagtccg acaatccgag cgccttcacg
     5641 caatctcgac ccgcgcgcca tccgcgtttc cgcccgccgt gcttcgccgc gggtctccga
     5701 ggcgcttccg cgcgcccgaa taggatcgcc atggaaaccg tgaccttcga atccctgggc
     5761 ctcgagcccg agatcctgga agccctggac cgcaagggct tcgaggagcc cacccccatc
     5821 caggccctcg ccataccccg gctcctgtcc ggcggggcca atctcgtggc ccgggcccgc
     5881 accggtaccg ggaagaccgc ggccttcggc ctgcccctga tccagaatct ccgggttccc
     5941 accggggccg tgcgcgccct ggtcctggtt cccacccggg aactggccct ccaggtctcc
     6001 ggggaggttt cctccctgcg gccccacggg gactaccccc gggtcgcgcc gatctacggg
     6061 ggcgcctcct acggggagca gttccgccgc ctctcccggg gcgtggagat cgtcgtgggg
     6121 acgccgggga gggtgaaaga ccacatcgag cggggaagcc tttccctgga ggccctggag
     6181 tacttcgtcc tggacgaggc ggacgaaatg ctggacatgg gcttcctgga ggacatcgag
     6241 tggatcttcg agaaggcccc cgcggatcgc cgggttctgc tcttctccgc caccatgccc
     6301 aaggagatcc tccggatcgc caagacccgg ctgggggagt acgagatcgt ggcggacgac
     6361 tccggccccg tggagaccgc cctgaccgac cagatctgga tcgaagtccg ggaggcggac
     6421 aagctggagg tcctgtgccg cctggtggac aaggaggacg agttctacgg gatcgtcttc
     6481 tgcgccacca aggtggacac ggacgccctg gccaagagcc tgggcgagcg gggctacggc
     6541 gccgaggccc tgcacgggga cctatcccag gtggagcggg aaaaggtgct ccgtcgcttc
     6601 cggaccaaga ggaccacgat cctgaccgcc acggacgtgg ccgcccgcgg gatcgacatc
     6661 gatcgcctga ctcacgtcgt gaacttctcc ctgccccatg acccggattc ctatacccac
     6721 cggatcgggc gcacgggccg ggcagggaac accgggaccg cggtcaccct ggtcaccccc
     6781 gacgaatacc ggaggatctt tttcctgcgg aagaccgcgg gcgcggccct gaagaaaggc
     6841 gcggtgccgg aggtggacga ggtcctggag gccaagcgtc agcggatccg gggccgagtg
     6901 ctttcggccg ccgcggatct ggggatcctt tctgaggaaa cggagggaag cgcgggacag
     6961 gctcccgggg aggcagggga cgcctcggcg gagcccggca agaccggtga gaaggccggg
     7021 acgccgcagc actccgagga agcccgggag cgctggcgga tcctcgcccg ggagatctcc
     7081 gaaggcctgg atcccctgga cgccttggcc gcggccttgg cggcgggcta ctcctcggag
     7141 ctggaccccg ggcgctacgg cgacgtccgc acggatttcc tggacaagga agccacgacc
     7201 cggctcttca tcggggcggg ccgccgggac ggggcggacc gaaaggccat cgcggtcctg
     7261 gtcaaggacc tggccggcct tccggaccgc ctggtcggag gggtggaggt ctacgacgcc
     7321 ttctccttcg tgaccgtccc cttcgaggcc gcggagcgca tcatcgcgga ggcacggaag
     7381 tcgggaaagg gtcccgtcgt gaagatggcc ttcccgaagg atggcgcccc ggcacgacgg
     7441 ggaccgccgg cgtaccggaa attcaacccc ggggaccgag gcccccggga tcgggacggc
     7501 ttcagtcctc caggtcgaac tcgcggacgt cgaaccaata cccggtccgg ctgaccaggt
     7561 ccagctgggc ccgcacgatg ctggtgtggc cgtcctcgat ctccacgaag cggtggaaga
     7621 cggcccggga ctcctccgga agggcggcga ccagggaacg gtagaaggcg ctcgtctcct
     7681 cctccgcggc aagggcccgg gtcagggcgg cccgttcccc gccctcgtcc aggccggtcc
     7741 gggccgggga cagggaggag gcggcccgtt tcatcgcctc ctccaggcgg tcccggggag
     7801 gcaggacggt ctcggggccc cgggagtcca gcgtgcccgt ccttttc
//
LOCUS       DAOTEI010000168         7780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6625_length_7780_cov_4.736699, whole genome shotgun sequence.
ACCESSION   DAOTEI010000168 DAOTEI010000000
VERSION     DAOTEI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6625_length_7780_cov_4.736699"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..2026
                     /locus_tag="P5313_13700"
     CDS             383..2026
                     /locus_tag="P5313_13700"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY81469.1"
                     /translation="MTWISASVDLVLNLTLLVSLSILSGFMDKRLKKETLRRDLLQGA
                     LFAVTTLVGMSRPLVLGPGLIFDGRSVMISLCALYFGPSAAAVAAGASIAYRVILGGS
                     GTLMGVAVALSSAVIGIIARARIDPEHRPPSPTRLYVFGLAVHVAMLCMTVFVPDRGG
                     LLVLRRIGIPIIVLYPLATILAGKILSDHVEARRALEALRASEERFKLSMEASNDGLW
                     DLDVRTGSAYYNPAYFRILGYEPGDFPALGSSWSSLVHPEDRERALAGNRSCIEEGRD
                     FLETEFRMISKDGGVRWIYSRGKVIARDASGRALRMVGTHVDITDRKRIEEELKRNVE
                     EKEILLREVHHRVKNNLSIISSLLNLQSSMIKTPEQALAAFQNSRERIQAMSLVHEEL
                     YKSMDYSRVDMEEYVGKLLAQLAFAYGEDRGITLTRRIQNVELDVSTSIPCGLILNEL
                     ITNAYKYAFPEGRAGTILIDLRITPEEVAEMRVADDGIGMPAGIDLETSDSLGLTLVR
                     LLVDQLRGTLRMSTGDGTAFTIRFPAHEGSASGPGGDHA"
     gene            complement(2093..2644)
                     /gene="amrA"
                     /locus_tag="P5313_13705"
     CDS             complement(2093..2644)
                     /gene="amrA"
                     /locus_tag="P5313_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRY81470.1"
                     /translation="MNFKLSQEERQTLLKTARESVESRLQGREPRWPAVPDSLAVPRG
                     AFVTLTAGGHLRGCIGRLTPSGPLIETVRSMARAAAFEDPRFPSLDREEWPRVRIEIS
                     VLGPMERLASVEDVVIGVHGLYIVQGFRAGVLLPQVATEWGWDRDEFLAQVCRKAGLP
                     AGAWKDPTAELHRFEAAVFGEED"
     gene            complement(2641..3477)
                     /gene="amrB"
                     /locus_tag="P5313_13710"
     CDS             complement(2641..3477)
                     /gene="amrB"
                     /locus_tag="P5313_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRY81471.1"
                     /translation="MIENSGSAKLREAVVSGIFYPEDPDALRAAVEGELGKAAAGRGD
                     AAAILSPHAAFEYAGAVAAAAWAAASRRVPSAVVLLAPYHRAEESAVWLPEAEVFQTP
                     LGDVMVDRAYVEELESCGTLFRQNDIPHFEEHGIEVQLPFLQVLFPKASVVPVLLGKP
                     SPSAVESLAKALSLVFSDSRESVLFVVSTNFAGHSSAEEASRRFERVLSYLASGDYEA
                     LYEEYRSETPDMCGVGCLASLMRSRLLGGRTWKLLERADSGSKRSTPAERIVYYAGGV
                     WV"
     gene            3539..4876
                     /locus_tag="P5313_13715"
     CDS             3539..4876
                     /locus_tag="P5313_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY81472.1"
                     /translation="MQVLLVDDEPNLLRSLEGFLKTEGIGCHTAPEGRAAQTILQERT
                     VDAVVLDLRMPGMDGMTLLRWIKESGPDVPVIMASAHGDVGDAVQAMKLGAYDYLVKP
                     FDPDELVLRLRKAVRERALSNGAEAERRSTEERVELVGTSPPILEAVRLIEKAAVTQA
                     TILLTGESGTGKEVAARLAHQASRRSGPFIAVNLGAIPDTLLESELFGHEKGAFTGAD
                     SRKQGLFELAQGGTLFLDEIGELPLQLQVKLLRALQERKIQRLGGVQGIPVDARIVAA
                     TNRDLEAMVREGRFREDLYYRINVIRIVLPPLRARTGDVALLADYFIRKLSGAMGRRV
                     DGLAPEARALLEGYRFPGNVRELQNAVERALILSDKTVLEAGDFQFFAPSAPGEDAED
                     LRDPGTGTLAELERRAILAALDRNSGRREQTARELGITRRTLLTKLKEYGVSP"
     gene            complement(4877..7234)
                     /locus_tag="P5313_13720"
     CDS             complement(4877..7234)
                     /locus_tag="P5313_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HRY81473.1"
                     /translation="MSERIRELREESFTARPSVSAERARILTDVYRNNEGKVSVPVLR
                     ALAFKGLCERKTVYLDPRELIVGERGPRPKACPTYPELTCHSVEDLQVLRTRPMTSYD
                     VDDSVIETYRTEIIPYWRGRSIRDRAFADIPSEWKALYEAGLFTEFMEQRAAGHTALD
                     GKLYRTGLAEFRERILARKTALDPSDPESGPKAEELTAMAIVCEGAIRFAERHAEKAE
                     ELAESERDPSRRADLLAVAETCRRVPALPPRTFREALQAYWFVHLGTITELNGWDAMS
                     PGHLDQHLAPFYEKDLAEGRLTRESAKELVSCFWIKVNNTPAPPKVGVTAAESGTYND
                     FTNINLAGLTPDGRDGSSEVSLILLEVLDELRLLQPQANVQVSARTPDRVLKEACRVI
                     RNGSGYPSLFNADEVVLAQAAMGKRLTDAREGGTSGCVETGCFGKEAYVLHGYLNVPK
                     ILELALNDGTDPVTGRKIGPSTGRPESFRNFEDLYTAFRAQLEYVVDWKVKVSEDLDR
                     MYGQNVPAPFLSVYIDDCIQTGKDYYCGGARYNTDYIQCVGLGTVTDSLSAIRTHVFE
                     GRSLGFPELLAALRDDWKGHEALRLLIRNKTPLFGNDDDRADALAVRVLDTLIETIEG
                     RKSWRGGRYYVDMLSTTCHIYFGLKTGATPDGRPARAPESDGTSPAHGSDRFGPTAVM
                     KSLGKLDQARTGGTLLNQRFLPATLAGEEGIDKLASLVRGYFRMGGHHVQFNVVDEAT
                     LRDAQKRPEAHRGLLVRVAGYSDYFVDLDRNHQEEIIARTAQEGF"
     gene            complement(7236..7628)
                     /locus_tag="P5313_13725"
     CDS             complement(7236..7628)
                     /locus_tag="P5313_13725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81474.1"
                     /translation="MADLFLYDLKHLDSREHERWTGVPNDRILENLRSLVEGGARIWV
                     RIPLIPGVNDGNDNLEATAEYLASIGFGREGSSGSSIQILPYHDSARRKYALRNLDYP
                     FTASPGGTSPQGAVELFRDRGLCARIGG"
     gene            complement(7645..7780)
                     /locus_tag="P5313_13730"
                     /pseudo
     CDS             complement(7645..7780)
                     /locus_tag="P5313_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1345 a         2497 c         2571 g         1367 t
ORIGIN      
        1 ggaacccttt cacgcattct attcccggga tcttcttccc catatcgagg cgtgtttcgc
       61 gcgcggaacg gctgcggata ggtcgtgttc catagcctcc gtgcttcggg gacatccgca
      121 catccggttc ccggaatccc gggtcagggc gatgttcccg gactggcggc ttttcctcaa
      181 catcaacacc ccggaagact gggaatcgta ccgggatccc gaatagccca agcgtaccga
      241 atcgaaagga agccgccggg tacctttcgg attcagtcgg ggcggatgcg aattagcatg
      301 gaaaaacgag gcacaatact cgatactgat ttcagcctcg ggggtgtcga gccataccgc
      361 cccggaccat gggagacttc cggtgacctg gatttccgcc tccgtggatc tcgtcctgaa
      421 ccttacgctt ctggtatccc tcagcatcct ttcggggttc atggacaagc ggctgaagaa
      481 ggaaacccta cgcagggacc tccttcaagg cgccttgttc gcggtgacca cgctcgtcgg
      541 catgtcccgg cctctcgtgc tcggacccgg tttgatcttc gacggccgtt ccgtgatgat
      601 aagcctgtgc gccctctact tcggcccctc cgcggcggcc gtggcggccg gagcctccat
      661 agcatatcgg gttatactgg gcggcagcgg gaccctgatg ggggtggccg tggccctctc
      721 gtcggccgtc atcggcatca tcgcccgggc gcgtatcgat cccgagcatc ggccgccttc
      781 cccgactcgg ttgtacgtct tcggccttgc cgtgcacgtc gctatgctgt gcatgacggt
      841 cttcgtgccc gacaggggag gcctcctggt gctccgcagg atcggcattc cgatcatcgt
      901 tctctacccc ctggcgacca tcctggccgg gaagatcctt tcggatcatg tggaggctcg
      961 gcgcgccctg gaagccctaa gggccagcga ggaacggttc aagctcagca tggaggcctc
     1021 gaacgacgga ctctgggacc tggacgtccg gaccggcagc gcttattaca atcccgcata
     1081 cttccgcatc ctgggatacg agcccgggga ctttcccgcc ctgggaagct cctggagttc
     1141 cctggtccac ccggaagatc gcgagcgggc cctggcgggg aaccggagct gcatagagga
     1201 aggccgtgat ttcctggaga cggaattccg catgatctcc aaggacggag gggtgcgctg
     1261 gatctactcc cgaggaaagg tcatcgcccg ggatgcgtcc gggcgggccc tgcggatggt
     1321 cggaacccac gtggacatca cggaccggaa acggatcgag gaggagctga aaaggaacgt
     1381 cgaggaaaag gagatactgc tgagggaggt tcaccacagg gtcaagaaca acctgagcat
     1441 catctcgagc ctgctgaacc tgcaatcgtc catgatcaag acccccgagc aggccctggc
     1501 cgccttccag aacagcaggg agcgcatcca ggccatgtcc ctggtccacg aggaactgta
     1561 caagtccatg gattactccc gggtcgacat ggaggaatat gtcggcaagc tcctggccca
     1621 gctcgccttc gcgtacgggg aggatcgcgg aatcaccctg acccggcgga tccagaacgt
     1681 ggaactggac gtctccacct cgatcccctg cggcttgatc ctgaacgagc tcatcacgaa
     1741 cgcatacaag tacgcgttcc ccgagggtcg ggcgggcacc atcctcatag acctccggat
     1801 cacgccggag gaggtcgccg agatgcgggt cgcggatgac gggatcggta tgcccgccgg
     1861 gatcgacctt gaaacgtccg attccctggg gctgaccctg gtgcggctac tggtcgacca
     1921 gcttcggggc actctgcgca tgagcacggg agacggcacc gccttcacca tccgattccc
     1981 cgcccatgag ggatccgcat ccggccccgg aggcgatcat gcttgaaggg gaaatccggc
     2041 ggccgatagt taagcctgcc ttgttcgcgg atcgttacgc ggaaaaccgg gactagtctt
     2101 cctcgccgaa tacagccgcc tcgaagcggt gaagttccgc ggtgggatcc ttccaggctc
     2161 ccgcgggaag ccccgccttc ctgcaaacct gggccaggaa ctcgtcccgg tcccagcccc
     2221 actccgtggc cacctggggc agcaggaccc cggcgcggaa tccctgaacg atataaaggc
     2281 cgtgaacgcc tatgaccacg tcctccacgg aggctaggcg ctccatggga cccaggacgg
     2341 agatctcgat ccggactcgg ggccactcct ccctgtcgag ggacgggaac cgcggatcct
     2401 caaaggccgc cgcccgcgcc atggagcgta cggtttcgat caggggaccc gagggggtga
     2461 gcctcccgat gcagccgcgc agatgtccgc cggccgtcag ggtcacgaag gcccctcggg
     2521 gtacggccaa ggagtcggga acggcgggcc atcggggctc ccgtccctgg aggcgggatt
     2581 ccacggattc ccgggcggtt ttcagcaagg tttgccgctc ctcctgggat agcttgaaat
     2641 tcatacccag actcccccgg cgtagtacac gatccgttcc gcgggtgtgg atcgcttgga
     2701 accggaatcc gcgcgttcca ggagcttcca ggtcctgcct cccagaaggc gggaacgcat
     2761 caaggacgcc agacatccta cgccgcacat gtccggcgtc tcgcttctat attcctcgta
     2821 cagggcttcg tagtccccgg aggcgagata cgaaagaacc ctctcgaagc gccgggaggc
     2881 ttcctccgcg gaggagtgcc cggcgaagtt cgtagatacc acgaagagaa ccgattcccg
     2941 ggaatccgag aaaaccaggc tcagggcctt ggccagggat tcgaccgcgg agggcgaggg
     3001 ctttcccagc aagacgggaa ccacggacgc cttcgggaag aggacctgca ggaagggcag
     3061 ctggacctcg atcccgtgtt cctcgaaatg ggggatgtcg ttctgtcgga agagggtgcc
     3121 gcaggactcc agctcctcaa cgtaggcccg gtcgaccatc acgtccccca ggggggtctg
     3181 gaagacctcg gcctcgggga gccagaccgc ggattcctcc gcccggtggt agggtgccag
     3241 aagcacgacc gcggagggga cccggcggga cgccgcggcc caggccgcgg ccgcgacggc
     3301 tccggcgtat tcgaacgccg cgtgggggga taggatcgca gcggcatcgc cccgacccgc
     3361 ggcggccttc ccgagttcgc cttccacggc ggcccggagg gcgtccggat cctcggggta
     3421 gaagatgccg gagaccacgg cttcccgaag cttggccgat ccgctattct cgatcatgag
     3481 aataagtata tggccggaat tcccgagcgc aagttcggat cggcattaag agggtgccat
     3541 gcaggttctt cttgtggacg acgagccgaa tcttctccga tccctggaag ggttcctgaa
     3601 gaccgaggga atcgggtgcc acacggcgcc ggagggacgg gcggctcaga ccattctcca
     3661 ggaaaggacc gtcgacgcgg tggtcctgga tctcaggatg cccggcatgg acgggatgac
     3721 cctgctccgc tggatcaagg aatccggtcc cgacgtcccc gtcatcatgg cctccgccca
     3781 cggggacgtg ggggacgccg tgcaggccat gaagctggga gcctacgact acctggtcaa
     3841 gcccttcgat ccggacgagc tcgttctgcg tctccggaag gccgtgcgag agcgtgccct
     3901 gtcgaacggc gccgaggcgg agcgtcggtc cacggaggag cgggtggaac tcgtcggtac
     3961 cagccccccg atcctggaag ccgttcgatt gatcgagaaa gccgccgtga cccaggctac
     4021 catcctcctg accggggaga gcgggacggg caaggaggtg gcggcccgcc tggcccatca
     4081 ggcttcccgg aggtccggcc ccttcatcgc cgtgaacctg ggggcaatac cggacaccct
     4141 tctggaaagc gaactcttcg gacacgaaaa aggagccttc acgggggcgg actcccggaa
     4201 gcaggggctc ttcgagctcg cccagggggg gacgttgttc ctggacgaga tcggggagct
     4261 ccccctgcag ctccaggtca agctcctgcg ggccttgcag gagcggaaga tccagcgcct
     4321 cgggggcgta caggggatcc ccgtggacgc caggatcgtg gcggccacga accgcgacct
     4381 ggaagccatg gtccgggagg gacggttccg ggaggacctg tactacagga tcaacgtcat
     4441 ccggatcgtc ctgccccccc tccgcgcccg aaccggggac gtcgccctgc tggcggacta
     4501 ctttatccgc aagctctccg gagccatggg ccgtcgggtg gacggcctgg ccccggaggc
     4561 gcgggccctt ctggagggct acagatttcc cggcaacgtg cgggaactgc agaacgcggt
     4621 ggaacgggcc ctgatcctct cggacaagac ggtcctggag gcgggggact tccagttctt
     4681 cgccccctcg gcgccgggcg aggatgcgga ggaccttcgg gatccgggaa cgggaaccct
     4741 cgcggagctg gagcgcaggg ccatcctggc cgccctggac cggaactccg ggcgcaggga
     4801 gcagacggcc cgggagctgg ggatcacccg caggaccctg ctcacgaagt tgaaggagta
     4861 cggagtctcg ccgtagctag aatccttcct gagcagtccg ggcgatgatc tcctcctggt
     4921 ggttgcggtc caggtccacg aagtaatccg aatatcccgc cacccgcacc aggagacccc
     4981 ggtgggcctc ggggcgcttc tgcgcgtccc gtagggtggc ctcgtccacg acgttgaact
     5041 ggacgtgatg accccccatc cggaagtatc cccggaccag ggaggccagc ttgtcgattc
     5101 cctcttcccc cgccagggtg gcgggaagga agcgctggtt gagcagggtt cctcccgtac
     5161 gggcctggtc gagctttccc aggctcttca tgaccgcggt ggggccgaag cggtccgatc
     5221 cgtgggccgg actggtgccg tcggactccg gtgccctcgc cggccttccg tccggagtgg
     5281 cgccggtctt gaggccgaag tagatatggc aggtcgtgga gagcatgtcc acgtaatacc
     5341 ttccgccccg ccaggatttc cggccctcga tcgtctcgat gagggtgtcc aggacccgga
     5401 cggccagggc gtccgcgcgg tcgtcgtcgt tgccgaacag gggggtcttg ttgcggatca
     5461 gcagccgcag ggcctcgtgc cccttccagt cgtcccggag tgccgcgagg agctccggga
     5521 acccgaggct ccggccctcg aagacgtggg tccggatggc ggagaggctg tccgtgacgg
     5581 tgcccaggcc cacgcactgg atgtaatcgg tgttgtaccg ggcacccccg cagtagtagt
     5641 ccttccctgt ctggatgcag tcgtcgatat atacggacag gaagggggcc gggacgttct
     5701 gtccgtacat ccggtccagg tcctcgctca ccttgacctt ccagtccacc acgtattcga
     5761 gctgggcgcg gaaggcggta tagaggtcct cgaaattccg gaaggactcc ggcctgcccg
     5821 tcgaggggcc tatcttcctg cccgtgacgg gatccgtgcc gtcgttcaag gccaattcca
     5881 ggatcttcgg cacgttcaga tagccgtgga gaacgtaggc ctccttcccg aagcagcccg
     5941 tctcgacaca gccgctggtc ccgccctccc gggcgtccgt caggcgtttg cccatggcgg
     6001 cctgggccag gacgacctcg tcggcgttga acagggacgg ataccccgat ccgttgcgga
     6061 tcacccggca ggcctccttg agcacccggt ccggagtccg ggccgacacc tgcacgttcg
     6121 cctggggctg gagcagccgg agttcgtcca ggacctccag gagtatcagg cttacttccg
     6181 aggagccgtc ccggccgtcc ggagtgagcc cggccaggtt gatattcgtg aagtcgttgt
     6241 acgttccgct ctccgcggcg gtgactccca ccttcggggg cgcgggggtg ttgttcacct
     6301 tgatccagaa acaggacacc agctccttgg cggattcccg ggtgagccgc ccctccgcca
     6361 gatccttctc gtagaacggg gccaggtgct ggtccaggtg gcccgggctc atggcgtccc
     6421 agccgttgag ctccgtgatg gtgccgaggt gcacgaacca gtaggcctgg agggcttccc
     6481 ggaaggtccg ggggggcagg gcgggtaccc ggcggcaggt ttcggccact gccaggagat
     6541 ccgcacggcg ggagggatcc cgttcggatt cggccagttc ctccgccttc tcggcatggc
     6601 gttccgcgaa ccggatcgcg ccttcgcaga cgatcgccat ggccgtgagt tcctcggctt
     6661 tcggaccgct ttccggatcg gagggatcca gggccgtctt acgggccagg atcctctccc
     6721 ggaattcggc cagacccgtc cggtagagct tgccgtccag ggccgtgtgg cccgcggccc
     6781 gctgttccat gaactccgtg aagagcccgg cttcgtacag ggccttccat tcggagggga
     6841 tatccgcgaa agcccggtcc cggatggacc ggccccgcca ataggggatg atctcggtcc
     6901 gataggtctc gatcacggaa tcgtccacgt cgtaggaagt catgggccgg gtcctcagga
     6961 cctgcaggtc ctccacggaa tgacaggtca gttcagggta ggtcgggcag gccttgggtc
     7021 ggggcccacg ctcccccacg atgagctccc ggggatccag gtagacggtc ttcctctcgc
     7081 agagtccctt gaaagccaat gcccgaagta cgggtacgct gaccttccct tcgttgttcc
     7141 ggtagacgtc cgtcagtata cgggcacgct ccgcggagac ggaggggcgt gcggtaaagc
     7201 tttcctcgcg cagttcccga atgcgttcgc tcatgtcatc ctccaatgcg ggcgcaaagg
     7261 ccccggtcgc ggaatagttc gacggccccc tgggggctcg tgcccccggg ggaggcggta
     7321 aagggatagt ccaggttgcg gagggcgtac ttgcggcggg cggagtcgtg gtaggggagg
     7381 atctggatcg aggaaccgga gctcccttcg cggccgaatc cgatggaggc gagatactcc
     7441 gccgtggctt ccaggttatc gttcccgtcg ttcactcccg ggatgagcgg aatccggacc
     7501 cagatcctcg ctccaccctc gaccagggac cgcaggttct ccaggatcct gtcgttcggc
     7561 accccggtcc atcgctcatg ttcccggctg tccaggtgct tgagatcgta cagaaagagg
     7621 tccgccatgg ccgccgcgct ctccctgata atgctgtttg aaacggtaat cgagaagggt
     7681 ttgaatacgg ggatccgcga cgaatgcaat gctgccgcca tggccgccgt caccgccatc
     7741 cgggcgcgga tatctcatcc acaggtcgcg gtagtggctt
//
LOCUS       DAOTEI010000169         7662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6773_length_7662_cov_5.226108, whole genome shotgun sequence.
ACCESSION   DAOTEI010000169 DAOTEI010000000
VERSION     DAOTEI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6773_length_7662_cov_5.226108"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2146)
                     /gene="uvrA"
                     /locus_tag="P5313_13735"
     CDS             complement(<1..2146)
                     /gene="uvrA"
                     /locus_tag="P5313_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRY81475.1"
                     /translation="MDKLIIKGAREHNLKNIDIELPRDKLVVVSGLSGSGKSSLAFDT
                     IFAEGQRRYVESLSAYARQFLGRMDKPDVDYIEGLSPAISIEQKSTHRNPRSTVGTVT
                     EIYDYYRLLYARIGIPHCPQCGREIKEQGIDQILDTILSWPKGSKLQILAPVVRGKKG
                     EHQKILEDARKQGFVRARVNGEIVSLEDEIRLEKQKKHSIEIIVDRIKLSEDSRKRLA
                     ESVETALQIAEDAAVVVRDSGQGETEEFFSRRGACPECGISLPELEPRLFSFNNPHGA
                     CPSCTGLGMNLEFDPNLVIPDPDLSFEEGGCIPYNPDAAWNRSRFEALAKHYKFSLGT
                     PFSRLPKKVMDAILYGTDDAVRVRYENREGTGHFEYESRFPGILADLKRRYMETTSDG
                     IKQWLERFMTEKPCEACGGRRLRPEALAVTVGGVNVHDLSARSVEATLDFFSNVELSD
                     TRRQIAKQILKEITARLTFMRNVGLEYLTLERKAATLSGGEAQRIRLATQIGSSLVGV
                     LYILDEPSIGLHQRDNQRLIDTLTYLRDIGNTLIVVEHDEQTLRTADWIVDLGPGAGV
                     HGGRVVAQGTPDQVMRSAKSLTGQYLAGTLRIDVPARRREGNGKSLVIRGCREHNLKG
                     IDVDLPLGKFVCITGVSGSGKSTLLTDLLYPAISNRVMGTSWPEGEFDSLEGIEHIDK
                     IINIDQSPIGRTPRSNPATYVGVFTHIRDLFAT"
     gene            complement(2233..3270)
                     /locus_tag="P5313_13740"
     CDS             complement(2233..3270)
                     /locus_tag="P5313_13740"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRY81476.1"
                     /translation="MSAKIEGKRRIRVAARVVLLLCLFGPSAAFGQEARAGFSPGSEL
                     PGHTGAVNSLAWSPDGGLLASAGGDRTCRIWDPSGGIPTAVVSIPAAVWGVSWAPDSR
                     RFAAASVDSVLRIFRRDGTLEREVRLEASGAYCVSWSPGGPRVAAGLSSGSVLVLDAD
                     TGQGTTFPVSDGSEGLSTQIIALAWSPDGRRIAAGGMDFVVRILDPQTGRIERELRAP
                     GEARKDVNGLAWSPDGTRIAVCQQDGRVRIWNADTGALIAASSQAGGWLRAVAWSPDG
                     RYLATAGNGRDIAVRDGSDGRILATIPAHRSPVWSLAWSPDGSRLASGSGQYATAGGD
                     CSVRIWDVRRD"
     gene            3303..3878
                     /locus_tag="P5313_13745"
     CDS             3303..3878
                     /locus_tag="P5313_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81477.1"
                     /translation="MSKNDEADHALHSLRREQLVEEVRRLRALLEDTRDLPDMASDAL
                     RRANARTEEYRTAFVVATEELARARSALQETWRVLKAQEFSAPAYSLYAETGGEALGA
                     TAGSPSLPEARERGACPACGGFGSHSPSCSLARALEPSRDPRPPRGELERAVDRAWEE
                     RTGEKDGVRARLVFLDVLFEELAKRGLVRED"
     gene            complement(4177..5274)
                     /gene="ychF"
                     /locus_tag="P5313_13750"
     CDS             complement(4177..5274)
                     /gene="ychF"
                     /locus_tag="P5313_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRY81478.1"
                     /translation="MPLNCGIIGLPTVGKTTIFTALTRVKAEISNYSSAKNPNLGIIA
                     VPDPRLDRLTEMFKPRKKVPATVEFVDIAGLASAASKKEGANQFLAHIRDSAVLAHVV
                     RCFEDPEVPHVSNRVSPGDDIEMLHLELALADLETVDKRAEKLQKELKDPAKKKDAER
                     ELAAYAKIRPALQEGRPARSVSLDAEEAEAVKRSFLITMKKELYVCNADEEGICTGSP
                     HVEAVRRIAEAEGAECVVICGKFESELADLESEEEKAEFLAELGLAESGLSALIHAVY
                     HLLGLRTFFTAGEDECRAWTIHVGDKAPAAAGTIHTDFEKGFIKAEVYRYDDLMEYGS
                     EHAVKSAGKLRLEGKEYVVQDGDVMFFKFNI"
     gene            5507..6742
                     /locus_tag="P5313_13755"
     CDS             5507..6742
                     /locus_tag="P5313_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY81479.1"
                     /translation="MASFESLYSENALNMKKSVIRELLKLTQKPDIISFAGGLPAPET
                     FPVEDLRAAADVVFVKHATKALQYGTTEGDPDLKDELIKFEARQGVKIGQDHLLVVSA
                     SQQALDIVAKVFIDPGDYIIGGHPTYLGTIQAIQSYRGRVLGIPFSKADDGFDMDELE
                     RRYASALRDGKRIKFIYVIPDFQNPTGICWSVEKRKALLEFSYRTGLPLVEDSPYREI
                     RFLGKTLPSIFQLDQEGENRGNVLNFKTFSKILAPGTRMGWVMANPDIISKFVVAKQA
                     MDLCTNVFAQKWIAEYLKTGKIYSVIEHTCGIYRDKRNYMTELLEQHMPKRPDISWTK
                     PEGGLFLWIRLPEGIDTDKMLLKAIERKVAYVGGSSFYFDEPEHNTMRINFSYSSLEQ
                     IEEGVKRLARVVAEELEGR"
     gene            6828..>7662
                     /locus_tag="P5313_13760"
     CDS             6828..>7662
                     /locus_tag="P5313_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY81480.1"
                     /translation="MLSNLVRQTAAGALLGAAVQSSLVLLLAPGTPLPALAASAAAGF
                     SVFLLAGTLVLRFGVEKPSARIADRVRTLAEGDLIGRVGEVRGHEALRTAAVELDETL
                     AGNYQVILLGLKEITRRNLEAARAFRADLDSAVRSIAAARDPVSSLGEKVGRLSARLS
                     SASSESKTVRASVTRLAERVADQAGAVEQSSAAIEETGSQIRSIAQTARKERQAALDL
                     AEAVERGGRGIGTVAEVIGGLSTGIAEVAELSRMINQVAARTNLLAMNAAIEAAHAGE
                     YG"
BASE COUNT         1234 a         2611 c         2531 g         1286 t
ORIGIN      
        1 gcgtggcgaa gaggtcgcgg atgtgcgtga agacgcccac gtaggtggcg gggttcgagc
       61 ggggggtccg gccgatgggg ctctggtcga tgttgatgat cttgtcgatg tgctcgatac
      121 cctccaggga atcgaactct ccctcgggcc aggaggttcc catcacccgg ttggagatgg
      181 ccgggtagag gaggtccgtg aggagggtgc tcttcccgga accggacacc ccggtgatgc
      241 agacgaattt ccccaggggc aggtccacgt cgatgccctt gaggttgtgc tcccggcagc
      301 ctcggatgac cagggacttg ccgttcccct cccggcgccg ggccgggacg tcgatccgca
      361 gggtcccggc caggtactgt ccggtaaggc tcttggcgct ccgcatcacc tggtcggggg
      421 tgccctgggc caccacccgg cccccgtgca ccccggcccc gggtcccagg tccacgatcc
      481 agtccgcggt ccggagggtc tgctcgtcgt gctccaccac gatcagggtg ttgccgatgt
      541 cccgcaggta ggtcagggtg tcgatgaggc gctggttgtc ccgctggtgc aggccgatgg
      601 acggctcgtc caggatgtac aggaccccca ccagggagga cccgatctgg gtggccaggc
      661 ggatccgctg ggcctccccg cccgagaggg tcgcggcctt tcgctccagg gtcaggtact
      721 ccagccccac gttccgcatg aaggtcaggc gggccgtgat ctccttcagg atctgcttgg
      781 cgatctgccg ccgggtgtcc gagagctcga cgtttgagaa gaagtcgagg gtggcctcca
      841 cgctccgggc ggacaggtcg tggacgttga cgccccccac cgtgacggcc agggcctcgg
      901 gccggaggcg gcggcctccg caggcctcgc agggcttctc ggtcatgaag cgttccagcc
      961 actgcttgat cccgtcgctg gtggtctcca tgtagcggcg cttaaggtcc gccaaaatgc
     1021 cggggaaacg gctctcgtac tcgaaatgtc ccgtgccctc ccggttctcg tagcggaccc
     1081 ggaccgcgtc gtccgtgccg tagaggatgg cgtccatgac ttttttgggc agacgactga
     1141 agggcgtgcc cagggagaac ttgtagtgct tggccagggc ctcgaagcgg ctgcggttcc
     1201 aggcggcgtc ggggttgtag gggatgcacc ccccctcctc gaaggagagg tccgggtccg
     1261 gaatgaccag gttcgggtcg aactccaggt tcatccccag ccccgtgcag gacgggcagg
     1321 ctccgtgggg attgttgaag gagaagaggc ggggctccag ctcgggcagg ctgattccgc
     1381 actccgggca ggccccccgg cggctgaaga actcctcggt ctcgccctgc ccggagtccc
     1441 ggacgacgac ggccgcgtcc tcggcgatct gcagggccgt ctccacgctc tccgccaggc
     1501 gtttccggga atcctcactg agcttgatcc ggtccacgat gatctcgatg ctgtgcttct
     1561 tctgcttctc cagccggatc tcgtcctcca gggagactat ctcgccgttc acccgcgccc
     1621 ggacgaagcc ctgcttccgg gcgtcctcca ggatcttctg gtgctccccc ttcttgcccc
     1681 ggaccacggg ggccaggatc tggagtttgg agcccttggg ccaggagagg atcgtgtcca
     1741 ggatctggtc gatgccctgc tccttgatct cccggccgca ctgggggcag tgggggatcc
     1801 cgatccgggc gtagaggaga cggtaataat cgtagatctc cgtaaccgtg cccaccgtgg
     1861 accgggggtt gcgatgggtg ctcttctgct cgatggagat ggccggggac aggccctcga
     1921 tatagtcgac gtcgggcttg tccatgcgcc ccaggaactg acgggcgtag gcggacaggg
     1981 actccacgta gcgcctctgt ccctccgcga agatcgtgtc gaaggccagg gagctcttgc
     2041 cggagccgga gagcccggag acgacgacca gcttgtcccg gggaagttcg atgtctatgt
     2101 tcttcaggtt gtgttcccga gcgcccttga tgatcagctt atccatgatg gctagagcat
     2161 acccaagccc gggcggccgg gcaaggggtg cttaatattt gtatagcgga ttgtagcggc
     2221 cgagctgtcc ggtcagtccc gacggacgtc ccagatccgt acggagcagt cccccccggc
     2281 cgtcgcgtac tggccggagc ccgaagccag gcgcgaaccg tcgggcgacc aggccaggga
     2341 ccatacgggg gatcgatggg cgggaatcgt cgccaggatc cggccgtcgg aaccgtcccg
     2401 caccgcgatg tcccggccgt tccccgcggt cgccagatac ctcccgtccg gggaccacgc
     2461 gaccgcccgg agccatccgc cggcttggga gctggctgct attagggcgc ccgtatccgc
     2521 gttccagatc cgcacccggc cgtcctgctg gcagacggcg atccgggtcc cgtccgggga
     2581 ccaggccagg ccgttcacgt ccttccgggc ctccccgggc gccctcagtt cccgttctat
     2641 ccgaccggtc tggggatcca ggatccgcac cacgaaatcc atgcctccgg ccgctatccg
     2701 acggccgtcg ggagaccagg ccagggctat gatctgggtg gacagcccct cggacccgtc
     2761 cgagacggga aaggtcgttc cctggcccgt atccgcgtcg aggacgagga cggatcccga
     2821 ggacaggccg gccgccacgc gcggtccccc gggcgaccag gagacacagt aggctccgga
     2881 cgcctccagc cggacttccc gttccagggt cccgtcccgg cggaagatgc gcaggacgga
     2941 atccacggac gccgcggcga agcggcggga atccggcgcc caggacactc cccagaccgc
     3001 ggccgggatc gagacgacgg cggtcgggat ccctccggag ggatcccaga tccggcaggt
     3061 cctgtcccct cccgcggagg ccaggagccc gccgtccggg gaccaggcca gggagttcac
     3121 ggctcccgta tggccgggga gttccgaccc cggggagaat ccggcccggg cttcctgccc
     3181 gaaggcggcg gacggcccga ataggcacaa cagcaggacg acccgggctg cgacgcgaat
     3241 cctgcgtttt ccctcgatct tcgctgacat ttcccgcttc cttcgctata gtttaccacg
     3301 aaatgtccaa gaatgacgaa gccgatcacg ccctccactc cctgcgccgg gagcagttgg
     3361 tggaggaggt ccgccgcctg cgcgccctcc tggaagacac tcgggacctt ccggacatgg
     3421 ccagcgacgc cctccgcagg gccaacgcac ggaccgagga ataccggacg gccttcgtgg
     3481 tggccacgga ggagttggca cgcgctcgtt cggccctcca ggaaacctgg cgggtactga
     3541 aggcccagga gttctccgcc cccgcctaca gcctgtacgc ggagaccgga ggcgaggctc
     3601 tcggcgcgac cgccggttct ccctccctac cggaggcccg ggagcgcggg gcctgcccgg
     3661 cctgcggggg cttcggctcc cattccccct cctgctccct ggcgcgggcc ctggagcctt
     3721 cccgggaccc ccggcccccg cgaggcgaac tggaacgggc cgtggaccgg gcctgggagg
     3781 agcggaccgg ggaaaaggac ggggtccgcg cccggctcgt cttcctggac gtgctcttcg
     3841 aggagctggc caagcgaggc ctggtccggg aagactgaac attgaatttg aagaacatca
     3901 cacccccgtc tcgtccggcg cggggcgccg gacggcgacg aacgcgttcg cgttcgtcga
     3961 tcccaccgga acgtcgtccg cgccggagcg ccgggcgctc cggcggcgcg gaaggcctct
     4021 aagccttccg cgatccaccg aatcgcgagt cttttagcta gatattaaac ttgaagaaca
     4081 tcacatcccc gtctcgtccg gcgcggggcg ccggacggcg acgaacgcgt tcgcgttcgt
     4141 cgatattcct gaaacgtcgt ccaggacggg cgggtatcaa atattgaatt tgaagaacat
     4201 cacatccccg tcctggacga cgtactcctt gccttccagg cggagtttgc cggcggactt
     4261 cacggcgtgc tcactgccgt attccatgag gtcgtcgtag cggtagacct cggccttgat
     4321 gaaacccttc tcgaagtccg tgtggatggt ccccgccgcg gcgggagcct tgtccccgac
     4381 atggatggtc caggcccggc actcgtcctc cccggcggtg aagaaggtgc gcaggcccag
     4441 gaggtggtag acggcgtgga tcagggccga caggccggat tccgccaggc ccagctccgc
     4501 caggaattcc gccttctcct cctcgctctc caggtccgcc agctcggact cgaacttccc
     4561 gcagatgacc acgcactcgg cgccctcggc ctcggcgatc ctccgcaccg cctcgacgtg
     4621 ggggctgccc gtgcagatcc cctcctcgtc ggcgttgcag acgtagagct ccttcttcat
     4681 cgtgatgagg aaggagcgct tgaccgcctc ggcctcctcg gcatccaggg agacggaccg
     4741 ggccggacgg ccctcctgca gggcggggcg gatcttggcg taggcggcca gttcccgttc
     4801 ggcatccttc ttcttggcgg gatccttcag ctccttctgg agtttttccg cgcgcttgtc
     4861 cacggtctcc aggtccgcca gggccagctc caggtggagc atctcgatgt cgtccccggg
     4921 gctcacccgg ttggagacgt ggggcacctc ggggtcctcg aagcaccgga ccacgtgggc
     4981 caggaccgcg gagtcccgga tgtgggccag gaactggttg gctccctcct tcttggaggc
     5041 cgcggaggcc agccccgcga tgtccacgaa ctccaccgtg gcggggacct tcttccgggg
     5101 cttgaacatc tccgtgagcc ggtccagccg tgggtccggc acggcgatga tgcccaggtt
     5161 gggattcttg gcggaggaat agttggagat ctccgccttg acccgggtca gggcggtgaa
     5221 tatcgtggtc ttgccgaccg tgggcagtcc gatgattccg cagttcaggg gcatatgtac
     5281 ctcgctcggg aggggcaccg gaccgctcac ggtccttcga cggccctttc ctacacccta
     5341 aagaaagcgc ctccccgcgt caatgccgga tcccgccgca gaaaaccgcg cttgcccggc
     5401 tccggacccg caacctttac gaagcggctc cgtatcggtt agcatgatcg gaactgccgg
     5461 accgggccgg tcgcccggaa cggcgaaacc accaaggaga cgatccatgg cttccttcga
     5521 atcgctctat tccgagaacg ccctgaacat gaagaaatcc gtcatccggg agctcctcaa
     5581 gctgacccag aagccggaca tcatctcgtt cgcgggcgga ctccccgcgc cggagacctt
     5641 cccggtggag gacctccggg ccgccgccga cgtcgtgttc gtcaagcacg cgaccaaggc
     5701 cctccagtac gggaccaccg agggcgatcc ggacctgaag gatgagctga tcaaattcga
     5761 ggcccgccag ggggtgaaga tcggccagga ccacctcctg gtcgtatccg ccagccagca
     5821 ggccctggac atcgtggcca aggtcttcat cgatccgggg gactacatca tcggcggcca
     5881 ccccacctac ctggggacca tccaggccat ccagtcctac cggggccggg tcctgggcat
     5941 ccccttcagc aaggcggacg acgggttcga catggacgaa ctggagaggc gctacgcctc
     6001 cgccctgcgg gacggcaagc ggatcaagtt catctacgtc ataccggact tccagaaccc
     6061 cacggggatc tgctggagcg tggagaagcg gaaggccctc ctggaattct cctaccggac
     6121 cggccttccc ctggtggagg attcccccta ccgggagatc cggttcctgg gcaagaccct
     6181 gccctcgatc ttccagctgg accaggaggg ggagaaccgg gggaacgtcc tcaatttcaa
     6241 gaccttctcc aagatcctcg ccccgggcac ccggatgggc tgggtcatgg cgaacccgga
     6301 catcatctcc aagttcgtgg tggccaagca ggccatggac ctgtgcacga acgtcttcgc
     6361 ccagaagtgg atcgccgagt acctcaagac cggcaagatc tactccgtca tcgagcacac
     6421 ctgcggcatc taccgggaca agcgcaatta catgaccgaa ctgctggaac agcacatgcc
     6481 caagcggccg gatatctcct ggaccaaacc cgaggggggc ctgttcctct ggatccgcct
     6541 ccccgagggg atcgacacgg acaagatgct gctcaaggcc atcgagcgca aggtcgcgta
     6601 cgtgggaggg tcgtccttct acttcgacga gcccgagcac aacaccatgc gcatcaactt
     6661 ctcctacagc tccctggagc agatcgagga gggagtcaag cgcctggctc gggtggtggc
     6721 cgaggagctc gagggccgct gatcccgacc cccgggattc cgagggaccg gaatcccggc
     6781 ttcgcgcttt catttgacgg acccggactt cggccctaga atggcccatg ctgtccaacc
     6841 ttgtcaggca aaccgccgcg ggtgccctcc tgggcgccgc cgtccagtcc tccctggtcc
     6901 tcctcctggc acccggcact ccccttcccg ccctggccgc ttccgccgcc gcgggatttt
     6961 ccgtcttcct cctggcgggc acgctggtcc ttcgcttcgg ggtcgaaaag ccctccgccc
     7021 ggatcgcgga tcgggtccgc accctggcgg aaggggacct cataggacgc gtcggcgagg
     7081 tgcgagggca tgaggccctc cgcaccgcgg cggtcgaact ggacgaaacc ctggcgggca
     7141 actaccaggt catcctcctg ggacttaaag agatcacccg ccgcaacctg gaggccgccc
     7201 gggccttccg tgcggacctg gattccgccg tccggtccat agcggccgcc cgggatcccg
     7261 tgtcctccct gggcgaaaag gtcggacgcc tttccgctag actgtcctcg gcctcctcgg
     7321 aatccaagac cgtccgggcc tccgtgaccc ggctggccga gcgggtcgcg gaccaggccg
     7381 gggcggtcga gcagtccagc gccgcgatcg aggaaaccgg gagccagata cggtccatcg
     7441 cccagacggc ccggaaggag cgccaggccg ccctggacct cgccgaggcc gtggaacggg
     7501 gcggccgggg gatcgggacg gtggcggagg tcatcggggg gctttcgacg ggtatcgccg
     7561 aggtggccga gctgtcccgg atgatcaacc aggtggccgc ccggacgaac ctcctggcca
     7621 tgaacgcggc catcgaggcg gcccacgccg gggagtacgg cc
//
LOCUS       DAOTEI010000170         7540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_6921_length_7540_cov_3.976754, whole genome shotgun sequence.
ACCESSION   DAOTEI010000170 DAOTEI010000000
VERSION     DAOTEI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6921_length_7540_cov_3.976754"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..705
                     /locus_tag="P5313_13765"
     CDS             13..705
                     /locus_tag="P5313_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRY81481.1"
                     /translation="MGERLEDVRIRIAKAAERSGRDPRTISILGVTKFHPLEAVQAAY
                     EAGIRVFGENRVQEALDKYPDFLVRRPDAEVHMIGHLQGNKVKKAVELFSCIQSVDSP
                     DLLGELERRAAAAGRRMDILLELHTGEESKSGFPDRGSALEACERLRGLGNLRLRGLM
                     TMAPYTDDAAAVRASFRALGALFEEIDTSGSFPDFDTLSMGMSNDFEIAVEEGATLLR
                     LGTVLFGKRDPA"
     gene            702..1160
                     /locus_tag="P5313_13770"
     CDS             702..1160
                     /locus_tag="P5313_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81482.1"
                     /translation="MRRFPAILAAAALAWTSAVPGARAQEAPAVPEPALPVRAAPDAL
                     PGAFVPEDRSPFLTGLRRFEIVAFGAFPIMLFYSNLGFDISRYARSGYDPFYAPWPLK
                     SEYSYKPTTPEILASIGTAALLSVGVGALDAVIRASRTRKAAAKARVDSP"
     gene            1182..1586
                     /locus_tag="P5313_13775"
     CDS             1182..1586
                     /locus_tag="P5313_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81483.1"
                     /translation="MKATKPKTLIVYEDAREDLAQAARDMERLAEAAGAAVRLRTAST
                     VTVSEVLASRLFVFGAHAPDSPSYAELRRLFRGINLAGRRAAFFGPPGSKGVAALKAS
                     LKDSDISLTGQDLDVGSGPAAVAAWFKTAMDF"
     gene            1605..2138
                     /locus_tag="P5313_13780"
     CDS             1605..2138
                     /locus_tag="P5313_13780"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRY81484.1"
                     /translation="MAQGFNLDDAIRKVPDFPKKGILFYDITSILMNPAAFEYCVDAM
                     VKLYKGEAVDAVAAIESRGFLFAAPFAQRLGLPLILVRKKGKLPGRTVSKSYALEYGQ
                     AEIEMHAEDIPAGGRVLIVDDLVATGGTLRAAADLLSEAGARPVGVFCVIGLPFLQYD
                     RVLEGLPVRTLIEYFGE"
     gene            2227..3885
                     /gene="gpmI"
                     /locus_tag="P5313_13785"
     CDS             2227..3885
                     /gene="gpmI"
                     /locus_tag="P5313_13785"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRY81485.1"
                     /translation="MLGSLKRDPNWKGRRGPVVLVVMDGVGYGAYEEGDAVRRALMPN
                     FRSLEASCPRTLLKAHGTAVGLPSDEDMGNSEVGHNAIGCGRVFAQGAKLVSASIDTG
                     AIFRGETWKKLVANVRASGGKLHFIGLFSDGNVHSHLDHLKALIGRAAEEGVKAVRIH
                     PLFDGRDVGEQSALDYLDPFEAFLAGFNGRGLDYRIASGGGRMYITMDRYGADWEMVR
                     RGWEAHVLGRGRAFPSAREAVLAYRAEKPSVIDQDLGEFVVVKDGKPVGIIEDGDSVV
                     YFNFRGDRALEITAAFERDDFDKFDRVRRPRVQYAGMMEYDGDLHVPAQYLVEPPSID
                     RTLGEYLAASKVRQLAISETQKFGHVTYFFNGNRTGKFDEALEDYVEIPSDRLPFEQR
                     PWMQCAQITDRLLEEIPSGKYGFIRVNFPNGDMVGHTGVFPAVVCSLEAMDLQIGRLR
                     AAVEKAGGILILTADHGNSDDMYEHDKKTGQVSRKPDGTPKAKTSHSLNPVPCVIYDP
                     EYKGEYETELASGLGISSLAATCARLLGFEPPEGYDPAVLRARG"
     gene            3888..4439
                     /gene="cyaB"
                     /locus_tag="P5313_13790"
     CDS             3888..4439
                     /gene="cyaB"
                     /locus_tag="P5313_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRY81486.1"
                     /translation="MLEVELKARIEDPGAVEGRVAAFARFVKKVDKSDSYWHGPGWRD
                     RRGTRGFRVRSEGDSFVVTFKSKRLEGGFEMNQEREFEISDPAVFDEFVRRIGCEPYY
                     RKRKVGAAYEYEGMTLEILRVEGAGTFLEIERLLATDSPAALEAAREGLKAALAKAGV
                     PESAIEKRTYSELILGKDGVSVS"
     gene            complement(4604..6226)
                     /locus_tag="P5313_13795"
     CDS             complement(4604..6226)
                     /locus_tag="P5313_13795"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRY81487.1"
                     /translation="MNVRIGIALTLAAVFDAAVGAEGGGMGGSEGKAVGDARGRVSAL
                     LLLDDEYGANFLIEDEIPSILEQLESYGWSLTTAAPKPEVRPCAWGSRNAGQKPFRPD
                     ITVRDLGDPSRYDVVILIPGKTQSNLQSDPGVLDFLRRAADRGTVIAAWCRSSRILAK
                     AGVLEGVRIVGKPEYRAEYEAAGAFPAEVPSPAETPPPVSDRGIVTTLRSKYYRTAMC
                     EEIRRTVESSVPRLSREREPPISRASSPAVAFFARAGQESEARILVESLRAFGGSLKD
                     APVSVLYPEDSPLRDGSDRRALERLGAELVPFRGDPRVMAFPLSDKAAAAAEAERRAE
                     GRADVLVWLDADTVFLDEPSDFLLPPGIALAARPVHHRLIGSPYDEPPSPLWKRLYES
                     EDVPESNLYPVVTTVDRQTIRPYFNAGCLVVRPERRVLRTWYAELARLIRDPAVVEEA
                     SEVPRGSLFLHQAVLSAVILSRIPREQIAELPFRYNYPLALHRSCPEDLRPASWSALT
                     TLRYDSLGNVRGDAWRILPAEGLSRKLRKLFE"
     gene            complement(6286..7182)
                     /locus_tag="P5313_13800"
     CDS             complement(6286..7182)
                     /locus_tag="P5313_13800"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY81488.1"
                     /translation="MDESGIGRGLCFLEDHLRKTFIMESAARAAGYSLYHFCRLFSLA
                     AGLPPGEYVRKRRLSEAARKLAETDEPVGRIAREYTFQSHEAFTRSFKALFGVSPERY
                     RRRTEPARLMKPFLLMEDFRISDPDRLARPPELVALDETRIAGLRFLCDLTSPRLASD
                     IRDHWERSAPRIRAAAGGVYPARRYGIGWPAEGRIEEPVLEYLAGMEWTEAPPPAGFR
                     TFRIPAGPYLRAIHRGAPALERETFLYLYGAYLPASNLDVRDAFDFDVYAERFHADSP
                     DDPDSEVEIYIPVKRREDPLRA"
BASE COUNT         1236 a         2539 c         2556 g         1209 t
ORIGIN      
        1 gactacggtt tcgtggggga acggctcgag gacgtacgga tccggatagc gaaggccgcg
       61 gaacgatcag gccgggatcc ccggacgata tcgatcctgg gggtgaccaa attccatccc
      121 ctggaggccg tccaggccgc ctacgaagcc gggatccggg tcttcggcga gaaccgggtc
      181 caggaggccc tggacaagta tccggacttc ctggtccggc ggccggacgc ggaagtccac
      241 atgatcggcc acctccaggg caacaaggtg aagaaggccg tcgagctgtt ttcctgcatc
      301 cagtccgtgg actccccgga cctcctgggg gaactcgagc gcagggccgc cgcagcgggc
      361 cgcaggatgg acatcctcct ggagctccac accggagagg aatccaagag cggcttcccg
      421 gaccgaggat ccgccctgga ggcctgcgaa aggctccgcg gcctcgggaa cctccggctt
      481 cgggggctca tgaccatggc cccgtacacg gacgacgcgg cggccgtccg ggcctccttc
      541 cgggccctgg gtgccctgtt tgaggaaatc gacacctccg ggtctttccc ggacttcgac
      601 accctgtcca tgggcatgtc caacgatttc gagatcgccg tggaggaggg ggcgaccctc
      661 ctgcgcctgg gcaccgtcct cttcgggaaa agggatccgg cgtgaggagg tttccggcga
      721 tcctggccgc ggcggccctg gcctggacat ccgccgtccc gggcgcccgg gcccaggaag
      781 cccctgcggt cccggagccg gcactcccgg tccgggccgc gccggatgcc ctgcccgggg
      841 ccttcgtccc cgaggaccgt tctcccttcc tgaccggcct gcggcggttc gagatcgtcg
      901 ccttcggggc cttccccatc atgcttttct attccaacct ggggttcgac atctcccggt
      961 acgcccggtc cggatacgat cccttctacg cgccctggcc gctcaagagc gagtactcct
     1021 acaagcccac cacccccgag atcctggcct ccatcgggac cgccgccctg ctttcggtgg
     1081 gcgtcggggc cctggacgcc gtgatccgcg cctcccggac ccgcaaggcg gcggcaaagg
     1141 cgagagttga cagcccctag cggccgtact agtatggaaa tgtgaaagcc accaaaccga
     1201 aaaccctgat cgtctacgaa gatgcccggg aggacctggc tcaggcggcc cgggacatgg
     1261 agcggctggc ggaagcggcc ggcgccgccg tccgcctccg gaccgcctcg accgtcaccg
     1321 tttcggaggt tctggcatct cgcctgttcg tgttcggggc ccacgctccg gattcccctt
     1381 cctacgcgga gcttcgcagg ctcttccggg ggatcaacct ggcgggccgc agggcggcgt
     1441 tcttcggccc cccgggatcc aagggagtgg cggcgctcaa ggcctccttg aaggactcgg
     1501 acatctccct caccggccag gatctcgacg taggatcggg acccgcggcc gtggccgcct
     1561 ggttcaagac ggcgatggat ttctgaccca acccgaggat tatcatggca cagggattca
     1621 acctcgacga cgccatccgc aaggtgcccg actttcccaa gaaaggcata ctgttctacg
     1681 acatcacgag catcctgatg aaccccgccg ccttcgagta ctgcgtggac gccatggtca
     1741 agctctacaa gggcgaggcc gtcgacgccg tggccgcgat cgagtcccgg ggcttcctct
     1801 tcgcggcccc cttcgcccag cgcctgggcc tgcccttgat cctggttcgc aagaagggaa
     1861 agctcccggg acgcacggtg tccaagtcct acgccctgga gtacggccag gcggagatcg
     1921 agatgcacgc cgaggacatt ccggccgggg gccgagtcct gatcgtggac gacctggtgg
     1981 ccaccggggg gaccctccgg gccgccgcgg acctcctttc cgaggcgggc gcccggcccg
     2041 tcggggtgtt ctgcgtcatc ggattgccct tcctccagta cgaccgggtc ctggagggcc
     2101 tgcccgtgcg gaccctgatc gaatacttcg gggaatgatc cggtcgttga aatcctcggc
     2161 ccgtttctat accttgtaac ccatcgacac gcaggcggtc cgttccgctg aaccctagga
     2221 ggggatatgc tcggctccct gaagcgcgat ccgaattgga agggacggag aggtcccgtg
     2281 gtcctggtgg tgatggacgg cgtcgggtac ggcgcatacg aggaagggga cgcggtgcgg
     2341 cgcgccctca tgcccaactt ccggtccctg gaggcgtcct gcccgcgaac cttgctcaag
     2401 gcccacggca ccgccgtggg gttgccctcg gatgaggaca tgggaaattc cgaggtcgga
     2461 cacaacgcca tcggctgcgg acgggtcttc gcccagggag ccaagctggt ctccgcctcc
     2521 atcgacaccg gggccatctt ccggggtgag acctggaaga agctcgtggc caacgtgcgg
     2581 gcttccggcg ggaagctcca cttcatcggc cttttttccg acgggaacgt acactcgcat
     2641 ctggaccacc tgaaggccct gatcggccgc gccgcggagg agggggtcaa ggcggtccgc
     2701 atccatcccc tcttcgacgg ccgggacgtg ggagagcaga gcgccctgga ctacctggat
     2761 cccttcgagg ccttcctggc cgggttcaac ggcaggggcc tcgactacag gatcgcctcc
     2821 gggggcgggc gcatgtacat caccatggac cgctacgggg cggactggga gatggtccgg
     2881 cgaggctggg aagcccacgt cctgggcagg ggccgggcct tcccgagcgc ccgggaggcc
     2941 gtcctggcct accgcgccga gaaaccctcg gtcatcgacc aggacctcgg ggaattcgtg
     3001 gtggtgaagg acggcaagcc cgtcggaatc atcgaggacg gggactcggt ggtgtacttc
     3061 aacttccggg gggaccgggc cctggagatc acggcggcct tcgagcggga cgacttcgac
     3121 aagttcgacc gggtacgccg tcccagggtc cagtacgcgg ggatgatgga gtacgacggg
     3181 gacctccacg tgcccgccca gtacctggtg gagcccccct ccatcgaccg gaccctgggg
     3241 gagtacctgg cggcgtccaa ggtccgccag ctggccatct cggagaccca gaaattcggg
     3301 cacgtcacct acttcttcaa cgggaaccgc accgggaagt tcgacgaggc cctggaggac
     3361 tacgtcgaga tccccagcga ccggcttccc ttcgagcagc ggccctggat gcagtgcgcc
     3421 cagatcacgg accggctcct cgaggagata ccctccggaa agtacggctt catccgggtc
     3481 aacttcccca acggggacat ggtcgggcac accggggtct tcccggcggt ggtctgctcc
     3541 ctggaggcca tggacctcca gatcggccgc ctgcgcgccg ccgtggagaa ggccgggggc
     3601 atcctgatcc tgaccgcgga ccacgggaac tcggacgaca tgtacgagca cgacaagaag
     3661 accggccagg tttcccggaa gcccgacgga acccccaagg ccaagaccag ccactccctg
     3721 aaccccgtgc cctgcgtgat ctacgacccc gagtacaagg gcgaatacga gaccgagctg
     3781 gcgtcgggcc tgggcatcag ctccctggcg gccacctgcg cgcggcttct gggcttcgag
     3841 ccccccgagg gctacgaccc ggcggtcctc cgggcgaggg gctgaccgtg ctggaggtgg
     3901 agctcaaggc ccggatcgag gatcccgggg ccgtggaggg gcgggtcgcc gccttcgccc
     3961 ggttcgtcaa gaaggtggac aagagcgatt cctactggca cggcccggga tggcgggacc
     4021 ggcgcggaac ccgaggtttc cgggtgcgct ccgaggggga ttccttcgtg gtcaccttca
     4081 agtccaagcg cctcgagggc gggttcgaga tgaaccagga gcgggaattc gagatctcgg
     4141 accccgcggt cttcgacgag ttcgtgcgga gaatcggctg cgagccgtac taccgcaaac
     4201 gaaaggtcgg agcggcctat gaatacgagg ggatgaccct ggagatcctc cgggtcgagg
     4261 gcgccggtac attcctggag atcgaacgcc tgcttgcgac cgactccccg gccgcgctgg
     4321 aggcggcccg ggagggactc aaggccgccc tggccaaggc gggggtcccc gaatccgcga
     4381 tcgagaagcg tacctactcg gaattgatac tcgggaagga cggggtctcc gtttcctgag
     4441 gccccccggt cgggcggcgg gccccggtcg ggagaacccc gggcggccta tcgcgaaaac
     4501 gctcctcgag cccggggtgt ccactacgtc ggctactcat tggacacccc ttgcagtttc
     4561 taacgttctc aatagaacgt taaaactgct accttcaaac cagctattca aaaagctttc
     4621 tgagctttct ggatagcccc tccgcgggga ggatcctcca cgcgtccccg cgcacgttcc
     4681 cgaggctgtc gtacctcagg gtggtcaggg cgctccagga agcggggcgc aggtcttcgg
     4741 ggcagcttcg gtgcagggcc aggggatagt tgtaccggaa gggcagttcc gcgatctgct
     4801 ccctgggtat ccgggacagg atgaccgccg aaagcaccgc ctggtggagg aagagggagc
     4861 cccggggaac ctccgaggct tcctcgacca ccgcgggatc ccggatcaac cgggcgagct
     4921 ccgcgtacca ggtcctgagg acccgccgct ccgggcggac gaccagacag ccggcgttga
     4981 agtagggccg gatcgtctgc cggtccacgg tggtcaccac ggggtacagg ttcgattccg
     5041 ggacatcctc cgactcgtag agacgtttcc agagcgggga gggcggctcg tcgtagggag
     5101 acccgatgag cctgtggtgg accggccggg ccgccagggc gatccccggg ggaagcagga
     5161 aatccgaggg ctcatccagg aagaccgtgt ccgcgtccag ccagaccagg acatccgccc
     5221 ggccttcggc ccggcgctcc gcctccgcgg ccgcggccgc cttgtccgac agggggaagg
     5281 ccatgaccct gggatcgccg cggaacggca cgagctcggc tcccagcctc tccagggccc
     5341 ggcggtccga gccgtcccgg agcggggaat cctccgggta aaggacgctg accggggcgt
     5401 ccttgaggga acccccgaag gcgcgaaggc tctcgaccag gatccgggcc tcgctctcct
     5461 gcccggcccg ggcgaaaaag gcgacggccg gggaggaagc ccgggagatc ggaggctcgc
     5521 gctcccggga aagccgcggt accgaggact ccacggtcct gcggatttcc tcgcacatcg
     5581 ccgtccggta gtacttgctc cggagggtcg tgacgatccc ccggtcggaa accggcgggg
     5641 gggtctccgc cggggacggg acctccgccg ggaaggcgcc cgcggcctcg tactcggccc
     5701 tgtattccgg cttgccgacg atgcgcacac cctccagcac ccccgccttg gccaggatgc
     5761 ggctggaacg gcaccaggcg gcgataacgg tccctcggtc cgcggcccgt cgaaggaagt
     5821 ccaggacccc cgggtcggac tggagattgg actgggtttt cccggggatg aggattacga
     5881 catcgtaccg ggacgggtcc cccaggtccc gtaccgtgat gtccgggcga aaaggtttct
     5941 gaccggcgtt ccgggagccc caggcgcagg ggcggacttc cggcttggga gccgccgtgg
     6001 tgaggctcca tccgtagctc tccagctgct ccaggatgct cgggatttcg tcctcgatca
     6061 ggaagttcgc gccgtactcg tcgtccagga ggagcagcgc ggacaccctg cccctcgcgt
     6121 cccctaccgc cttgccttcc gagcccccca tgccgcctcc ttccgcgcct acggccgcgt
     6181 cgaagaccgc ggcgagcgtc agggcgattc cgattcgtac gttcattccc cgcctccgcc
     6241 gtgagtatac cggctccccg ggccgtcggg atttgatagt tcttgctaag cccggagcgg
     6301 gtcttcccgg cgcttcacgg gaatgtagat ctccacctcg gaatccggat cgtccggact
     6361 gtccgcatgg aagcgttcgg cgtatacgtc gaaatcgaag gcgtcccgga catccaggtt
     6421 cgaggcgggg agataggccc cgtagaggta caggaaggtt tcccgttcca aggccggggc
     6481 tccccggtgg atcgcccgta ggtaggggcc ggccgggatg cggaaggtcc ggaagcctgc
     6541 gggcggcgga gcttccgtcc attccatgcc ggccaggtat tccaggaccg gctcctcgat
     6601 ccgaccctcg gcgggccagc cgatgccgta ccgccgggcg gggtagacgc ctccggcggc
     6661 ggcccggatc cggggagcgg accgttccca atggtcgcgg atgtccgagg ccaggcgggg
     6721 cgaggtcagg tcgcagagaa agcgcaggcc ggcgatccgg gtttcatcga gcgccacgag
     6781 ttccggcggc cgggccaggc ggtcggggtc ggagatccgg aaatcctcca tgaggaggaa
     6841 gggcttcatg agccgcgccg gctccgtgcg gcgacggtac cgttccgggc tgacgccgaa
     6901 aagggccttg aaggacctcg tgaaggcctc gtgggactgg aaggtgtatt cccgtgcgat
     6961 ccttcccacg ggttcgtctg tctcggccaa tttccgggcc gcctcggaca gcctgcgctt
     7021 ccggacatat tcccccggag gcaggccagc cgccagggag aagagcctgc agaagtggta
     7081 cagcgagtat cccgccgccc tggccgcgga ctccatgatg aaggttttcc gcagatgatc
     7141 ctcgaggaag caaagcccac gcccgattcc agattcgtcc atcccgcacc ccgccggtct
     7201 tcaccgccga cgggacagta tatctcggga gcggaatgcg cgcgacgtca ttcctcagcg
     7261 tgtctaatgt tgaacgccgc cttgcggcgg cgttcttcgg ttgggcgctg agggattgcc
     7321 ctccagccct cggcatcctg ccgcgggctt ccgggccggg gtcggcgctc gcgctcgcca
     7381 tcctggctcg ctcacctcgc tgccgctcgg cgcgccgacc cttcaaatcc ctcagcgccc
     7441 cccaagtacg aaaccccgac cggccgaagg ccgatcgggg tttcgtatgg gcgctgaggg
     7501 atttgaaccc ccgacatcct gggtgtaaac caggcgctct
//
LOCUS       DAOTEI010000171         7453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7036_length_7453_cov_4.641390, whole genome shotgun sequence.
ACCESSION   DAOTEI010000171 DAOTEI010000000
VERSION     DAOTEI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7036_length_7453_cov_4.641390"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="P5313_13805"
     CDS             <1..812
                     /locus_tag="P5313_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY81489.1"
                     /translation="GIVADGMLVFADRPLALVGIAEKGYVDVELEARGAGGHASMPPR
                     STAAGTLARAVARLESRPFPARLTYTVRSFLSELSPYVPFGFRLLFRSPGVSAFALIK
                     AFSAAPTTNALVRTTQAATMLSGSDKENVLPDRARAVINVRILPGQTVAGALARIASI
                     VARDGVTVRQAHEGLAVEPLPESPTDHEGYRALRSALGAAHPEAAVVPFLFSAGTDSK
                     HYADLVQAIYRIAPIRQTSRDLAGVHGPDEKISFENLRRCALFYYELMKGM"
     gene            complement(895..1392)
                     /locus_tag="P5313_13810"
     CDS             complement(895..1392)
                     /locus_tag="P5313_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81490.1"
                     /translation="MDRDRSEGGEATPSRSPAERVLRITILVLAAALVLGTVYALATD
                     TRGRAARSSPGPRIDVPREGEAVYSRLGTIRASTRDDPPAVVAVSIAFPYPAGDRAFK
                     EELDDKAARLREACAAFFAEKSAESLHPAFEGALKSELRDRLNAQLSLGRIRELWFGD
                     FAVVR"
     gene            complement(1545..2816)
                     /locus_tag="P5313_13815"
     CDS             complement(1545..2816)
                     /locus_tag="P5313_13815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY81491.1"
                     /translation="MPSWKRSFYVIWFAELIAIAGFNTTTPIIPLFLRDLGITDPGRL
                     NYWNGLSQSASALALALLAPVWGSLADGYGRKPMILRAMFGGAALISLMALTTEPWQV
                     FALRTLQGCVTGTVAAATVFVASIVPKEEAGYRLGLLQMAIFLGGAVGPLFGGFVADA
                     AGPRVNFLATGAFLALSGLLVLRFVREDFIPVPRPGSIFRNALPDLSPAVRNPALAAL
                     LLVTFAVQFASQVAVSIMTLLVFHLLGDVPTVGRVSGLLIGAGTLAAALSAAAVGRVS
                     GRVGYGRTLMGCIAGALVFYVFQGFARTPAQLFWFRIGSGIFLGGTMPSVNALIARLC
                     EPGRQGSTYGLSSSVSSAGAALGPAVGALVAATAGYPFVFFTASAILLGTGLIVVRAS
                     AHGITFSSGSPSGAPDPEPRTGSKASGPEGG"
     gene            2949..3968
                     /locus_tag="P5313_13820"
     CDS             2949..3968
                     /locus_tag="P5313_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81492.1"
                     /translation="MSAENGAWDSEGRPTPRKVILLAAAFLAALAFVLVVRRPESQSR
                     FLSYREGGNLVFLDKDLPGPLRTAIRRDRVPRSDAFLAALGYRGGSVADRDLVVVAAD
                     GGYGALAADDPVVARRLSGRASAELLLLARMSRDFFANEPVSRSNISADGRYVFLDAR
                     EPWEPSWIHALAHSLLVDSRGPGARARVEAGVNPGLFSPEAARGWEFLQESAALLAEG
                     FFVLSGGDPGRLAEAAAGYGPSGAARFGPEGPVRAELALREALAVPPPRKDAAWYEAC
                     HGFGAWLLAEYGLDAVRGFLGHGLGGTWETLDDLVRPFGTDFPGLLNRWSGGKGLPPP
                     YRQDT"
     gene            complement(4099..5901)
                     /locus_tag="P5313_13825"
     CDS             complement(4099..5901)
                     /locus_tag="P5313_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81493.1"
                     /translation="MNTKRNAPGAVLLRLLPYYRPYAFILAADLLAVLAVAALSLALP
                     LVLRSLVDDVIPSGDVRRLAELSGIVAALALGKFAANFYTLYAGHAYAVAMERDLRRD
                     FFAKLTRMSFSFFDDRKTGELMSRMSNDINKVSDAVNHVPEDILLTLATTAGAFGVLA
                     SLDPTLAVISFLPIPLAALYSGPLGGRILAGFEKANDAVAGVSVKIESVVSGMRTVQA
                     FAREPHEIRRFADLNRAIYQAWRGVLLSLGFFFGGADFLKDLARLVVTAAGGWFALRG
                     RISPGTLVAFLFYANLILEPVDRLTRTVEASQRLAAGLKNFFEILDQEPAIRDRPGAL
                     PLDRPEGHIRFEDVTFGYARGRHVFRGLTADIAPGSTVALVGPSGAGKTTFCSLIPRF
                     YEPLEGRVLLDGRDVREYRLADLRKAVGIVQQDVFLFPGTVRENIRYGRPEASEAEVE
                     DAARSANAHEFILGLPSGYDTDIGERGIKLSGGQKQRLSIARAFLKDPAVLILDEATS
                     SLDMKSEQAVQEALRRLSVGRTTLIIAHRLSTVRAADEILVLTEEGIVQRGGHEELLA
                     AGGVYADLYALQTEGVSAEEDEEDEEAEGGELGA"
     gene            6309..6725
                     /locus_tag="P5313_13830"
     CDS             6309..6725
                     /locus_tag="P5313_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRY81494.1"
                     /translation="MRHEISCTWTGDMSFEAEVSGHKIRLDADEAVGGKDSGPRPKPL
                     VLVALAGCSGMDVVSILKKMREPVTWFDVKVGGDLTEEHPKYYSSVKITYEFKASDGL
                     TDENVRKAVALSQDKYCGVNALLKKAIPVEWEVVYK"
     gene            7047..>7453
                     /locus_tag="P5313_13835"
     CDS             7047..>7453
                     /locus_tag="P5313_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRY81495.1"
                     /translation="MAFDVKKMRNIGISAHIDSGKTTLSERILFYCQKIHAIHEVRGK
                     DAAGATMDFMELEKERGITIQSAATNVTWKDHAINLIDTPGHVDFTIEVERALRVLDG
                     AVLVLCAVGGVQSQTLTVDRQLKRYHVPRLAFI"
BASE COUNT         1267 a         2502 c         2558 g         1126 t
ORIGIN      
        1 gggggatcgt cgcggacggt atgctggtct tcgcggaccg gcccctggcc ctggtaggga
       61 tcgcggaaaa gggctacgtg gacgtggagc tggaggcccg gggcgcggga gggcacgcct
      121 ccatgccgcc ccggagcacg gccgcgggca ccctggcccg ggcggtggcc cgcctggaat
      181 cccggccctt cccggcccgg ctgacctaca cggtgcggag cttcctctcg gaactctctc
      241 cctacgtgcc cttcggattc cgcctcctgt tccggagccc cggcgtatcg gccttcgccc
      301 tgatcaaggc cttctccgca gcgcccacca cgaacgccct ggtcaggacc acccaggccg
      361 ccaccatgct ctccgggagc gacaaggaga acgtcctgcc cgaccgggcc cgggcggtca
      421 tcaacgtgcg catcctgccc ggccagaccg tcgcgggggc gctggcccgg atcgcctcca
      481 tcgtggcgcg ggacggggtg acggtccgcc aagcccacga ggggcttgcc gtggagcccc
      541 tgccggaatc cccgacggac cacgaggggt accgggcgct gcgctcggcc ctgggggcgg
      601 cccacccgga ggcggcggtg gttcccttcc tgttcagcgc gggaaccgat tccaagcact
      661 acgcggacct cgtccaggcc atctaccgga tcgcgccgat ccgccagacc tcgcgggacc
      721 tggcgggggt gcacggcccc gacgagaaga tctccttcga gaacctccgc cgctgcgccc
      781 tgttctacta cgagctcatg aaggggatgt agcgcgcccg cgcccggcct cactccgata
      841 gaatcgccga agcgaggctt cggcgcgttg aagggcgggg cgcaagcccg cccttcaacg
      901 gacgaccgcg aagtcgccga accagagctc ccggatgcgc cccagggaga gctgggcgtt
      961 gagccggtcc cggagctcgg acttcagggc cccctcgaag gcgggatgga gggattccgc
     1021 gctcttttcc gcgaagaagg ccgcgcaggc ctcccgcagg cgggccgcct tgtcgtccag
     1081 ctcctccttg aaagcccggt ccccggcggg gtacggaaag gcgatgctga ccgccacgac
     1141 cgcgggggga tcgtcccggg tggaagcccg gatggttccc agtcgggaat agaccgcttc
     1201 cccctcccgg gggacgtcga tccggggacc cggggaggat cgggcggccc ggccccgggt
     1261 atccgtcgca agggcgtaga ccgtgcccag gaccagcgcc gccgccagga ccaggattgt
     1321 gatccgcagg acccgctccg cgggactccg ggacggggta gcctccccgc cctccgatcg
     1381 atcgcgatcc atgcctacct cctaggagcc tgtcggactt gtggattttt agcgccgata
     1441 ggcgctaaaa atcccgatta tgcggctcct ttggagtttg caaggtagaa aaatcgcaca
     1501 aatgcgattt ttctactatt taggtgggca agcccgacaa actcctagcc gccttccgga
     1561 ccggatgcct tggaacccgt ccggggctcc ggatcgggcg ccccggacgg ggacccggac
     1621 gagaaggtga tgccgtgggc cgatgcccgg accacgatca ggccggtacc caacaatata
     1681 gccgaagcgg tgaagaatac gaagggatac ccggcggtcg ccgcgaccag ggctcccacg
     1741 gcgggtccga gggccgcccc ggcgctggag acggagctgg acagcccgta cgtggagccc
     1801 tgacggcccg gttcgcagag ccgggcgatc agggcgttca cggagggcat ggtcccgccc
     1861 aggaagatcc ccgagccgat gcggaaccag aagagctggg cgggtgtccg ggcaaagccc
     1921 tggaatacgt agaaaaccag ggctcccgcg atgcagccca tcagggtcct gccgtacccc
     1981 accctcccgg aaaccctgcc caccgcggcc gcggacaggg ccgcggccag ggtgcccgcg
     2041 ccgatgagca ggccgctgac cctcccgacg gtcgggacgt cgccgagcag gtggaacacc
     2101 agcagggtca tgatggaaac cgccacctgg ctggcgaact ggaccgcgaa ggtcaccagg
     2161 agaagggcgg ccaaggccgg attccggacc gccggggaca ggtcgggcag ggcgttccgg
     2221 aaaatcgatc cgggccgggg gaccgggatg aagtcctccc tcacgaagcg caggaccaac
     2281 aagcccgaaa gggccaggaa cgcgccggtt gccaggaagt tgacccgggg acccgcggcg
     2341 tccgctacga agcccccgaa caggggaccc acggctcctc ccaggaaaat ggccatctgc
     2401 aaaaggccca ggcgatagcc cgcctcttcc ttggggacga tggaggccac gaacaccgtg
     2461 gccgcggcca cggttcccgt gacgcagccc tggagggtcc ggagggcgaa gacctgccag
     2521 ggttccgtgg tcagggccat caggctgatg agggccgccc cgccgaacat ggcccgcagg
     2581 atcatgggct tgcgtccgta tccgtccgcc aggctccccc agaccggggc cagcagggcc
     2641 agggcaaggg cggaggcgga ctgggaaagc ccgttccaat agttcaaccg cccgggatcg
     2701 gtgatcccga ggtcccggag gaagaggggg atgatggggg tggtcgtgtt gaacccggct
     2761 atggcgatca gctcggcgaa ccagatgacg taaaaggatc gtttccagga tggcatgatg
     2821 ggaccggaac ccagtgtacc cgcaaaggag cgggagaggc aacctggaat cgccgggatt
     2881 ccggcccgcc cgcggacgga catcgacgag gggccggccg gggtttacaa tgaaggggga
     2941 ggcgtccaat gtccgcagag aatggtgcct gggactcgga gggaaggccc accccccgga
     3001 aggtgatcct cctggccgcc gccttcctgg cggccctggc cttcgtcctc gtcgtgcgcc
     3061 ggccggaatc ccagagccga ttcctgtcct accgggaagg cgggaacctc gtgttcctgg
     3121 acaaggacct gcccgggccc ctgcgaaccg cgatccgcag ggaccgggtc ccccggtcgg
     3181 acgccttcct ggcggccctg ggctaccgcg gcggaagcgt cgcggaccgg gacctggtcg
     3241 tggtcgccgc ggacggcggg tacggggccc tggccgcgga cgatcccgta gtcgcccggc
     3301 gcctctcggg gagggcctcc gcggagctcc tcctcctggc ccggatgtcc cgggatttct
     3361 tcgccaacga gccggtctcc cgctcgaaca tctccgcgga cggccggtac gtcttcctgg
     3421 acgcccggga accctgggag ccctcgtgga tccacgccct ggcccattcc ctcctggtgg
     3481 actcccgggg gcccggggcc cgggcccggg tcgaggccgg ggtgaatccc gggctcttct
     3541 ccccggaggc cgcccggggc tgggagttcc tgcaggaatc cgcggccctc ctggcggagg
     3601 ggttcttcgt cctgtccggc ggggatcccg ggcgcctggc cgaggccgcc gcgggctacg
     3661 gcccctccgg ggccgcccgc ttcgggccgg aaggtccggt ccgggccgag ctggccctcc
     3721 gggaggcctt ggccgtcccg cccccccgga aggacgcggc ctggtacgag gcctgccacg
     3781 gcttcggggc ctggctcctg gcggaatacg gcctggacgc ggtccgcggc ttcctgggcc
     3841 acggcctcgg gggaacctgg gagaccctgg acgacctggt ccggcccttc gggacggact
     3901 tccccggcct tcttaaccgg tggagcggag gaaaaggact tccccccccc taccggcagg
     3961 atacctagca gggtgttgaa aaagactata gagccttttt caacacttcc ttaatgtcgc
     4021 aattgcgcag agaaccgcaa ggttctcgag caattgcata gcaggttgtt gaaatgcctc
     4081 aagtatttca acaacctgct aggcgccgag ctcccctccc tccgcctctt cgtcctcctc
     4141 gtcctcctcc gcggagacac cctccgtctg gagggcgtag aggtccgcgt acaccccccc
     4201 cgcggccagg agctcctcgt ggcctccccg ctggacgatc ccctcctccg tgagcaccag
     4261 gatctcgtcc gcggcccgca cggtggaaag ccggtgggcg atgatcaggg tggtgcggcc
     4321 gacggaaagc cgccgcaggg cctcctggac cgcctgctcg ctcttcatgt ccagggagct
     4381 cgtcgcctcg tccaggatca ggaccgcggg atccttcagg aaggcccggg ctatggagag
     4441 cctctgcttc tggccccccg acagcttgat cccccgctcc ccgatgtccg tgtcgtaccc
     4501 cgagggcaga cccaggatga actcgtgggc gttcgcggac cgggccgcgt cctcgacctc
     4561 ggcctccgag gcctcgggcc ttccgtaacg gatgttctcc cgcaccgtgc cggggaagag
     4621 gaagacgtcc tgctggacga tccccacggc cttccggagg tccgccaggc ggtactcccg
     4681 tacgtcccgg ccgtccagga ggacccggcc ttccaggggc tcgtagaacc gggggatcag
     4741 gctgcagaag gtcgtcttcc cggcgccgct gggacccacc agggccacgg tggagcccgg
     4801 ggctatgtcc gcggtgaggc cccggaagac gtgccggccc cgggcgtacc cgaaggtgac
     4861 gtcctcgaag cggatatgtc cctcgggccg gtccaggggc agggctcccg ggcggtcccg
     4921 gatcgccggt tcctggtcca ggatctcgaa gaagttcttg agccccgcgg ccaggcgctg
     4981 ggaggcctcc acggtccggg tcagccggtc cacgggctcc aggatgaggt tggcgtagaa
     5041 gaggaaggcc accagggtgc ccgggctgat cctgccccgg agggcgaacc agccccccgc
     5101 ggcggtgacc acgaggcggg ccaggtcctt gaggaagtcc gccccgccga agaagaagcc
     5161 cagggacagg aggacccccc gccaggcctg gtagatggcc cggttgaggt cggcgaagcg
     5221 acggatctcg tggggctccc gggcgaaggc ctggacggtc cgcatgcccg agaccacgga
     5281 ctcgatcttg acgctcaccc cggccacggc gtcgttggcc ttttcgaaac ccgccaggat
     5341 cctccctccc agggggccgg aatacagggc cgccaggggg atgggcagga aggagatcac
     5401 cgccagggtc gggtccaggg aggccaggac cccgaaggcc ccggccgtgg tagccagggt
     5461 caggaggatg tcctccggga cgtggttcac cgcgtcggag accttgttga tgtcgttgga
     5521 catccgggac atgagctccc cggtcttccg gtcgtcgaag aaggagaagg acatccgggt
     5581 gagcttggcg aagaagtccc tgcgcaggtc cctctccatg gccacggcgt aggcgtggcc
     5641 cgcgtacagg gtgtagaagt tggccgcgaa cttgcccagg gccagggccg ccacgatccc
     5701 cgagagttcc gccagtcggc ggacgtcccc ggaggggatc acgtcgtcca ccagcgaccg
     5761 gaggaccagg ggcagggcca gggagagggc ggccacggcc aggaccgcca ggaggtcggc
     5821 ggccaggatg aaggcgtagg gcctatagta cggaagcagg cgcagaagga cggcgccggg
     5881 ggcgtttctc ttggtgttca tgtcgcgtcc aagggccgcc tcggcgccgt gcaggtgccg
     5941 ggggcccggt atccggtgac tcaccccggg acgatgccgg gggcgagggc tcaggagccc
     6001 ggtaaacggg cttcttttgg ggccgagcct gagacgagat accggttgga cgccgaggga
     6061 ggcgggggcc tccggttcgg gcggctggaa tccggtccgt caggcccggg aggcgcgaac
     6121 tcgggtcgcg ccgaccatga cactgtaacc gttcctcatc gtataccgct ccttttcggg
     6181 cccctaagga cccggtttcc ttggacggac cgtattctac atccgtaccg aggggcttcg
     6241 tcaagtctcc gcgcgaattg ccccggagct atccgatttc gtatactgga aggacgagga
     6301 ggaacggaat gaggcacgag atttcctgta cctggaccgg ggacatgtcc ttcgaggcgg
     6361 aggtctctgg gcacaagata cggctggacg ccgacgaggc cgtcggaggc aaggactcgg
     6421 gtccccggcc caagcccctc gtcctggtcg ccctagcggg atgctccggc atggacgtgg
     6481 tctccatcct caagaagatg cgggagcccg tcacctggtt cgacgtcaag gtcggcgggg
     6541 accttacgga ggagcacccc aagtactatt cctccgtcaa gatcacctac gagttcaaag
     6601 cctccgacgg ccttacggac gagaacgtcc ggaaggccgt ggccctgtcc caggacaagt
     6661 actgcggcgt gaacgcccta ttgaagaaag ccatcccggt ggagtgggaa gtggtgtata
     6721 agtgaataag tgaataggtg aatgggttaa tggggcgccc aaggatccgg ccgctcccaa
     6781 cgctatttta aatcttccgc ggattcctgt gcttcctcct attcactatt cacagattca
     6841 cgattcacct cctacttgaa acttctcccc atcctctata cactgcggac gggttacggt
     6901 ttttcaaagg aattacggaa aaccgcaaga actgcaacat agtgtgcgtt ttcatccccc
     6961 aaaagtttcg ttggacgcaa ggagccgggg ccgctcggtt acgggcccgt gagatagaac
     7021 cccattctgc gaggtagagg aagagcatgg ctttcgacgt gaagaaaatg cggaatatcg
     7081 gcatcagcgc ccacatcgac tcgggcaaga cgaccctgtc cgaacgcatc ctgttctatt
     7141 gccagaagat ccacgccatc cacgaggtcc ggggcaagga cgccgccggc gccaccatgg
     7201 acttcatgga attggagaag gagcggggga tcacgatcca gtccgcggcc accaacgtga
     7261 cctggaagga ccacgcgatc aacctgatcg acacccccgg ccacgtggac ttcaccatcg
     7321 aggtggagcg cgccctccgg gtcctggacg gggccgtcct ggtcctctgc gccgtgggcg
     7381 gggtccagtc ccagaccctc acggtggacc gccagctcaa gcgctaccac gttccccggc
     7441 tcgccttcat caa
//
LOCUS       DAOTEI010000172         7353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7180_length_7353_cov_4.781584, whole genome shotgun sequence.
ACCESSION   DAOTEI010000172 DAOTEI010000000
VERSION     DAOTEI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7180_length_7353_cov_4.781584"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="P5313_13840"
     CDS             complement(<1..111)
                     /locus_tag="P5313_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRY81496.1"
                     /translation="MGQKVNPIGLRLGINKDWKSRWYVDPREYAKTLHEDL"
     gene            complement(113..469)
                     /gene="rplV"
                     /locus_tag="P5313_13845"
     CDS             complement(113..469)
                     /gene="rplV"
                     /locus_tag="P5313_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRY81497.1"
                     /translation="MNAGYVAKAKFLIASPSKVRPVADLIRRKPYTEAVAILDNMPHK
                     GARLIRKVVMSAAANALDRNRKLAEDMLFVKELRIDDGPRMRRVWFRARGRADMQLKR
                     MCHISVVVDEIGRTEA"
     gene            complement(478..759)
                     /gene="rpsS"
                     /locus_tag="P5313_13850"
     CDS             complement(478..759)
                     /gene="rpsS"
                     /locus_tag="P5313_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRY81498.1"
                     /translation="MSRSVKKGPFIEASLYKKIVEMTKGGDKKMVKTFSRTSTIIPEM
                     VGMTISVYNGKTWVPVYVTENLVGHKLGEFAPTRIFRGHAGSDKKAEKK"
     gene            complement(769..1599)
                     /gene="rplB"
                     /locus_tag="P5313_13855"
     CDS             complement(769..1599)
                     /gene="rplB"
                     /locus_tag="P5313_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRY81499.1"
                     /translation="MAIKTFKPITPGLRHRVQVVNTELTCGNEPEKALSRGSKRSSGR
                     ASNGRISMRRRGGGHKRKYREIDFLRDKIGIPGKVASIEYDPNRSANIALINYVDGEK
                     RYIIAPKGLTVGQKILSGPDAPIETGNTLPLESIPVGLTVHNVELNLGRGGQLARSAG
                     AGALIAAKEGDYVILKLPSGELRMVFKKCSATIGSVGNDEHMNERIGKAGRSRWMGMR
                     PRVRGVAMNPIDHPHGGGEGRGKGYKQPVTPWGQPCKGYKTRDDHKPSGSFIVKRRKG
                     "
     gene            complement(1626..1910)
                     /locus_tag="P5313_13860"
     CDS             complement(1626..1910)
                     /locus_tag="P5313_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRY81500.1"
                     /translation="MQYEDILIEPVLSEKTNEMREGGKFVFRVDPRATKTQVMEAVRR
                     LFQVHPVSCTVMNVKGKPKRLRNRAGYTPSWKKATVRLAAGETIKAFEGA"
     gene            complement(1921..2556)
                     /gene="rplD"
                     /locus_tag="P5313_13865"
     CDS             complement(1921..2556)
                     /gene="rplD"
                     /locus_tag="P5313_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRY81501.1"
                     /translation="MEGKLYSVQGKELGTVQLDDAVFGLPINEDVIYYAIHNELANAR
                     VGSACTKGRSEVHGTNRRPYAQKGTGRARQGDVKSPLYVGGGIVFGPKPRDYSYRMPR
                     KAKRLAIKTILSLKAQERVLKVVQDFTVPAGKTKDLASILKTVLPREKDERTVVILKD
                     DDADLKRAGRNIPWLHFLSYNRLRAHDLFYGRSVVVLESAVKSLNEFYSGN"
     gene            complement(2566..3198)
                     /gene="rplC"
                     /locus_tag="P5313_13870"
     CDS             complement(2566..3198)
                     /gene="rplC"
                     /locus_tag="P5313_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRY81502.1"
                     /translation="MIGIIGQKIGMTQVFDETGRITPVTVLKIAPNVVVGRKDKETFG
                     YEAVVFGFFDIKKKNVTKPYAGQFPEGVAPTRILRESKGFEGEVEVGKSVGVEYFQDV
                     RFVDVSANSKGKGFQGVVKRWGFGGGRATHGSKFHREPGSTGQRTYPHKTFKNVKLPG
                     RMGNDKVTVQNLKVVRVDAENGILLVRGAVPGPRNCAVTVRPAVKKMKRG"
     gene            complement(3242..3547)
                     /gene="rpsJ"
                     /locus_tag="P5313_13875"
     CDS             complement(3242..3547)
                     /gene="rpsJ"
                     /locus_tag="P5313_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRY81503.1"
                     /translation="MKDRIRVRLRGFDVRLIDDSARSIVTTVQKAGAKVAGPIPLPTR
                     INKYTVLRSPHVHKKSREQFEMRTHKRLIDIMDPTADVMDALMKLELPAGVDVEIKQ"
     gene            complement(3800..4990)
                     /gene="tuf"
                     /locus_tag="P5313_13880"
     CDS             complement(3800..4990)
                     /gene="tuf"
                     /locus_tag="P5313_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRY81504.1"
                     /translation="MAKEKFERTKPHMNVGTIGHIDHGKTTLSAAITMYCAKKFGDKV
                     MKYDDIDNAPEEKARGITINTRHLEYQSVKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     VLVVSAPDSVMPQTREHVLLARQVGVPSVLVFLNKVDLVDDAELIELVEQEIIDLLNF
                     YGFPGDKTPIIKGSAFKAMSEPDNAEATKCIQDLLDAMDSFFPDPERLTDKPFLMPIE
                     DVFTISGRGTVVTGRVERGIVKVGETVEIVGIKPTKSTVVTGVEMFNKLLDEGQAGDN
                     IGTLLRGVEKKDVERGQVLAKPGSITPHTKFKGRIYCLSKDEGGRHSPFFSGYRPQFY
                     FRTTDITGSVKLPEGREMVMPGDNTEIFVELIYPIAMEKGLKFAIREGGRTVASGQVI
                     EVQN"
     gene            complement(5205..5675)
                     /gene="rpsG"
                     /locus_tag="P5313_13885"
     CDS             complement(5205..5675)
                     /gene="rpsG"
                     /locus_tag="P5313_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRY81505.1"
                     /translation="MGRKKKTVDRGHQPDPKYNSVTVTRFVCRMMWEGKKATCSRIMN
                     NSMAILQEKAGVPALDAFLKALDNVKPLVEVKSRRVGGATYQVPIEIREVRREALAMR
                     WLLAAARGRSGKSMAERLSAELLDAFNNTGTAVKKKEDTHRMAEANKAFAHYRW"
     gene            complement(5687..6061)
                     /gene="rpsL"
                     /locus_tag="P5313_13890"
     CDS             complement(5687..6061)
                     /gene="rpsL"
                     /locus_tag="P5313_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRY81506.1"
                     /translation="MPTINQLIRLGRKTNTWRTKAPALDECPQRRGVCTRVMTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGAK
                     DTLGVEDRKKARSKYGAKRPKA"
     gene            complement(6291..>7353)
                     /locus_tag="P5313_13895"
     CDS             complement(6291..>7353)
                     /locus_tag="P5313_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRY81507.1"
                     /translation="EAKQPRVIITDEAGNEITTYFLPGGAYLNVEDGELVKAGRTLAK
                     TLKESAKAMDITGGLPRVSELFEARKPKTPTVLAMVAGTVAFRGIVKGKRVVVVRDIF
                     GKEYKHLVPMGRRLLVRDGDTVESGDLLCDGNKNPHDIMNILGEQYCQRFLMDEVQQV
                     YRLQGVTINDKHIGVIVRQMMKKVEIVSPGDTRFIYGQQIDKYKFHEENKRVISEGGQ
                     PAVARPLLLGITRASLKIDSFFAAASFQETTRVLTDAAIAGATDTLRGLKENVIIGHL
                     IPAGTGMRKYRNIKLFDQENDDLDAFVNEIIEKRKREKELQPLPALEDRGDGSAFEED
                     AVFENDEGFGQVGGEDDED"
BASE COUNT         1066 a         2344 c         2420 g         1523 t
ORIGIN      
        1 caggtcctcg tggagggtct tggcgtactc ccggggatcg acgtaccagc gggacttcca
       61 gtccttgttg atgccgagcc ggagcccgat cggatttact ttctggccca cgttacgcct
      121 ccgtccttcc gatttcgtcc acgactacgc tgatgtggca catgcgcttg agctgcatgt
      181 ccgcccggcc gcgggcgcgg aaccagacgc ggcgcatccg gggaccgtcg tcgatcctga
      241 gttccttgac gaagagcatg tcctccgcca gcttccggtt gcggtccagg gcgttggccg
      301 ccgcggacat gacgacctta cggatcagcc gggcgccctt gtggggcatg ttgtccagga
      361 tggccaccgc ctcggtgtag ggcttgcggc ggatcaggtc cgccaccggc cgtaccttgg
      421 agggcgacgc tatcagaaac tttgccttgg ctacgtatcc tgcgttcatg gcatccccta
      481 ctttttctcg gccttcttgt cgctgcccgc atggccgcgg aagatgcggg tgggcgcgaa
      541 ctcgccgagc ttgtgaccga ccaggttctc ggtgacgtac accggcaccc aggtcttccc
      601 gttgtagacc gagatggtca tgccgaccat ctcggggatg atcgtggagg tgcgggagaa
      661 ggtcttcacc atcttcttgt ccccgccctt ggtcatctcg acgatcttct tatacaggct
      721 ggcctctatg aagggcccct tcttgacgga tctcgacacg gttggctcct atcccttcct
      781 gcgcttgacg atgaagctgc ccgacggctt gtggtcgtcg cgcgtcttat agcccttgca
      841 cggctggccc cagggggtca cgggctgctt atagcccttg ccccggccct cgccgccgcc
      901 gtgggggtgg tcgatcgggt tcatggccac gccgcggacc cgggggcgca tgcccatcca
      961 ccggctgcgg cctgccttgc cgatccgctc gttcatgtgc tcgtcgttgc cgacggaccc
     1021 gatggtggcg gagcacttct tgaagaccat ccggagctcg ccggaaggca gcttgaggat
     1081 gacgtagtcg ccctccttgg ccgcgatcag ggcgccggcc ccggcggagc gggccagctg
     1141 gccgccccgg cccaggttca gctccacgtt gtggaccgtg aggcccacgg ggatggactc
     1201 cagcggaagg gtgttgcccg tctcgatggg ggcgtccggg ccggacagga tcttctgtcc
     1261 gacggtcagc cccttcgggg cgatgatgta gcgcttctcg ccgtccacgt agttgatgag
     1321 ggcgatgttg gcgctccggt tgggatcgta ctcgatggag gcgaccttcc cggggatccc
     1381 gatcttgtcc cgaaggaaat cgatctcccg gtacttgcgc ttgtgcccgc cgccccggcg
     1441 ccgcatggag atgcggccgt tggaggcccg gccggaggag cgcttggagc ccctggacag
     1501 ggccttttcc ggctcgttgc cgcaggtgag ctccgtgttg accacctgga cccggtgccg
     1561 gagtcccggg gttatcggct tgaacgtctt gatagccata tcttcccctt agttgccgtg
     1621 aaggctcacg cgccctcgaa ggccttgatc gtctcgcccg cggccagacg caccgtcgcc
     1681 ttcttccacg agggagtgta gccggcgcga ttccggaggc gcttcggctt gcctttgacg
     1741 ttcatcacgg tgcaggacac cggatgaacc tggaacagcc tccgcacggc ttccatgacc
     1801 tgggtcttgg tggcccgcgg atccacgcgg aagacgaact tgccgccctc gcgcatctcg
     1861 ttggtcttct cggagaggac cggttcgatc aggatatctt cgtactgcat cttcctctcc
     1921 tcaatttccc gagtagaact cgttgaggct cttgacggcc gactcgagca ccacgacgga
     1981 gcgtccgtag aacaggtcgt gagcccgcag gcggttgtag gacaggaagt ggagccaggg
     2041 gatgttcctg ccggcccgct tcaggtccgc gtcgtcgtcc ttgaggatga ccaccgtgcg
     2101 ctcgtccttt tcccggggca ggaccgtctt caggatggac gccaggtcct tggtcttgcc
     2161 ggcggggacc gtgaagtcct ggaccacctt caggacccgc tcctgggcct tgaggctcag
     2221 gatagtcttg atcgccaggc gcttggcctt ccggggcatc cggtaggagt agtcccgggg
     2281 cttcggcccg aacacgatgc cgccgcccac gtagagcggg gacttgacgt cgccctgccg
     2341 ggcgcggccg gtgcccttct gggcataggg ccggcggttg gtaccgtgga cctcggagcg
     2401 tcccttggtg caggccgagc ccacccgggc gttggccagc tcgttgtgga tcgcgtagta
     2461 gatcacgtcc tcgttgatgg gcaggccgaa gacggcgtcg tccagctgga cggtcccgag
     2521 ctccttgccc tgtacggaat atagcttgcc ttccatctcg gtcacctacc ctcgcttcat
     2581 tttcttgacc gcgggacgca ccgtgacggc gcagttccgc ggaccgggga cggcgccgcg
     2641 aaccaggaga atcccgtttt cggcgtccac ccggacgacc ttgaggttct ggaccgtcac
     2701 cttgtcgtta cccatgcggc cgggaagctt gacgttcttg aaggtcttgt gcgggtaggt
     2761 ccgctgtccg gtcgaaccgg gttcccggtg gaacttggac ccgtgggtgg ctcggccgcc
     2821 cccgaatccc cagcgtttca cgacgccctg gaagcccttg cccttcgagt tcgcggagac
     2881 gtccacgaag cgcacgtcct ggaagtactc cacgccgacg ctcttgccga cctcgacctc
     2941 gccctcgaag cccttggact cccggaggat gcgggtgggg gcgacgccct ccgggaactg
     3001 cccggcgtag ggcttggtca cgttcttctt ctttatgtcg aagaatccga agaccacggc
     3061 ttcgtaccca aaggtttcct tgtccttgcg gccgaccacc acgttgggtg cgatcttgag
     3121 gacggtgacg ggagtaatcc tgcccgtctc gtcgaagacc tgggtcatgc cgatcttctg
     3181 tccgatgatt ccgatcattc gatgctccta gcgttgaacg cttcggtacg taaccgggcg
     3241 tttactgctt gatctccacg tcgacgcccg cgggaagctc cagcttcatc aacgcgtcca
     3301 taacgtcggc ggtcggatcc atgatgtcga tgaggcgctt gtgggttctc atctcgaact
     3361 gctcccgcga cttcttgtgg acgtgcgggg aacgtaggac cgtgtacttg ttgattcggg
     3421 tcgggagcgg gatgggtccc gcgaccttcg cgcccgcctt ctggaccgtc gtcacgatcg
     3481 aacgagcgga gtcgtcgatg agacggacgt cgaatcccct gagccttacg cggatcctgt
     3541 ctttcatgct tcctccaaag atctcttgct tccggtcctt tcgtgccgtt cccgggaggg
     3601 cgtgcgccgg gggccggaag ccgcggggga ttgggccccc gcaagccgct tggcgaagcc
     3661 gccctggggc ggccgcgccg gaaacctgaa cgcgtcacgc cctcccgggc ggaacgccgc
     3721 ttcggccggg aacgcgaagc gcccccgatc gaagacgaag ggcgcgcgaa cttgcgtccg
     3781 cgcgcccgga atcccttact tagttctgaa cctcgatgac ctgaccggag gccacggtcc
     3841 gcccgccttc acggatggcg aacttgagcc ccttctccat ggcgatgggg tagatcagtt
     3901 cgacgaagat ctccgtgttg tccccgggca tcaccatctc acggccctcg ggcagcttga
     3961 cggaaccggt gatgtccgtg gtgcggaagt agaactgggg ccggtagccg gagaagaagg
     4021 gggaatggcg gccgccctcg tccttgctga ggcagtagat ccgtcccttg aacttggtgt
     4081 gcggggtgat ggaacccggc ttggccagaa cctggccgcg ctcgacatcc ttcttctcca
     4141 caccccggag cagggtgccg atgttgtcgc ccgcctgccc ctcgtccagg agcttgttga
     4201 acatctcgac gccggtgacg acggtgctct tggtcggctt gatgcccacg atctcgacgg
     4261 tctcgcccac cttgacgatc ccgcgctcca cacgtccggt cacgacggtg ccgcggccgg
     4321 agatggtgaa gacgtcctcg atcggcatga ggaagggctt gtccgtgagt cgctcggggt
     4381 ccgggaagaa ggagtccatg gcgtccagga ggtcctggat gcacttggtg gcctcggcgt
     4441 tgtccggttc ggacatggcc ttgaaggcgg aacccttgat gatgggggtc ttgtccccgg
     4501 ggaagccgta gaagttgagg aggtcgatga tctcctgctc caccagctcg atcagctccg
     4561 cgtcgtcgac caggtcgacc ttgttcagga agaccaggac ggaggggacg cccacctggc
     4621 gggcgagcag gacgtgctcg cgggtctggg gcatcacgga gtcgggagcg gacacgacca
     4681 ggaccgcgcc gtccatctgg gccgcgccgg tgatcatgtt cttgatgtag tcggcgtggc
     4741 cgggacagtc gatgtgcgcg tagtggcgct tcacggactg gtactccagg tgccgggtat
     4801 tgatggtgat accgcgggcc ttctcttccg gggcgttgtc gatatcgtcg tacttcatca
     4861 ccttgtcgcc gaacttcttg gcgcagtaca tggtgatggc ggcactcagg gtggtcttac
     4921 catggtcgat gtggccgatc gtaccgacgt tcatatgagg tttggtacgc tcgaatttct
     4981 ctttggccat tgaacttcct cctctagcta tatacggaac gatccagctc gcacctttgg
     5041 ggacacggca ttataacggc gagcccgtat cttgacaaga gcccgccgac gggtcctcgc
     5101 cctaaaatat cccgccggac gatgcaacgc atcgtcctgg gcagccggat ttggcctacg
     5161 gccaaatccg atccgtgaaa cgacgctatg cgtcgtttca cgggctacca gcgatagtgc
     5221 gcgaaggcct tgttcgcctc ggccatccgg tgggtgtctt ccttcttctt cacggcggtg
     5281 cctgtgttgt tgaaggcgtc caaaagctcc gcggacaggc gttcggccat ggacttgccg
     5341 gaccggcccc gggcggcggc caggagccag cgcatggcca gggcttccct gcgaacctcc
     5401 cggatctcga tgggcacctg gtaggtggcg ccgccgacgc ggcgggactt cacttccacc
     5461 aggggcttca cgttgtccag ggccttcaag aaggcgtcca gggcgggaac cccggccttt
     5521 tcctggagaa tggccatgga attgttcatg atccgggagc aggtggcctt cttgccctcc
     5581 cacatcatcc ggcagacgaa acgggtgacg gtcacgctgt tgtacttggg atcgggctgg
     5641 tgcccgcgat ccacggtctt tttcttgcgg cccatatccg tcccccttac gccttcggcc
     5701 gcttggcgcc gtacttggaa cgggccttct tgcggtcttc cacgccgagg gtgtccttgg
     5761 cgccgcggac gatgtggtag cgcacgcccg ggaggtcctt gacgcggcct ccgcggatga
     5821 ggaccaccga gtgctcctgc aggttgtggc ccacgccggg gatataggcg gtgacctcga
     5881 tgccgttcgt gaggcggacg cgcgcgacct tccggagggc cgagttcggc ttcttcgggg
     5941 tgacggtcat gacgcgcgtg cagacgccgc ggcgctgcgg gcactcgtcg agggccgggg
     6001 ccttggtccg ccacgtgttg gtcttgcgac ccagacggat aagctggttg atcgtcggca
     6061 tgtatgctct cgactccctt tgtcgtgcct ggcttcagca tagcccggcg aaatatggtg
     6121 gtacgcgata tgaagactgt ttcacgcctc cggccctcgc ggacccggaa gtctagaccg
     6181 taaccttacc cttccggggg ctccctagtc aaggagtcgc cgcccgggcc gggaccccgc
     6241 gggcggccgg ccggggcttt cgcccccggc cggcccctcc cgcgcgggcg tcagtcctcg
     6301 tcgtcctcgc cgccgacctg gccgaagccc tcgtcgttct cgaagacggc gtcctcctcg
     6361 aaggccgacc cgtcgcccct gtcctcgagg gcggggaggg gctggagctc cttctccctc
     6421 ttccgcttct cgatgatctc gttgacgaag gcgtccaggt cgtcgttctc ctggtcgaag
     6481 agcttgatgt tcctgtactt gcgcatgccc gtgccggccg ggatgaggtg gccgatgatg
     6541 acgttctcct tgaggccgcg gagcgtgtcc gtggcgccgg cgatcgcggc gtcggtgagg
     6601 acccgggtgg tctcctggaa ggaggccgcg gcgaagaagg agtcgatctt gagcgaggcg
     6661 cgggtgatgc cgaggaggag gggcctcgcg acggccggct ggccgccctc ggagatgacc
     6721 cgcttgttct cctcgtggaa cttgtacttg tcgatctgct ggccgtagat gaacctcgtg
     6781 tcgccggggc tcacgatctc caccttcttc atcatctgtc ggacgatgac gccgatgtgc
     6841 ttgtcgttga tcgtgacgcc ctggaggcgg tacacctgct ggacctcgtc catgaggaag
     6901 cgctggcagt actgctcgcc caggatgttc atgatgtcgt gcgggttctt gttgccgtcg
     6961 cagagcaggt cgccggactc gacggtgtcg ccgtcgcgca cgaggagcct gcggcccatc
     7021 ggcacgaggt gcttgtactc cttgccgaag atgtcgcgga ccacgacgac gcgcttgccc
     7081 ttcacgatgc cgcggaaggc gaccgtgccg gcgaccatcg cgaggacggt cggcgtcttg
     7141 ggcttgcggg cctcgaagag ctcgctgacc ctcgggaggc cgcccgtgat gtccatggcc
     7201 ttcgcggact ccttgagggt cttcgcgagg gtccggcccg ccttcacgag ctcgccgtcc
     7261 tcgacgttga ggtaggcgcc gcccggcagg aagtaggtcg tgatctcgtt gcccgcctcg
     7321 tcggtgatga tgacgcgggg ctgcttcgcc tcg
//
LOCUS       DAOTEI010000173         7314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7227_length_7314_cov_4.276347, whole genome shotgun sequence.
ACCESSION   DAOTEI010000173 DAOTEI010000000
VERSION     DAOTEI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7227_length_7314_cov_4.276347"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="P5313_13900"
     CDS             <1..443
                     /locus_tag="P5313_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81508.1"
                     /translation="RYQSADYTIAHRYLTYVNRRDFGPFRAITIGLGLDDRIAATAES
                     LASFLRNPPERTDRPLPGSPESGAAGSGPGQGAAAPGSGADPDASGAPGAGPDARNAA
                     SAGGIPWILAGLGAGVAALAILVLVVRARAGKEERKKKPEEPGA"
     gene            440..1417
                     /locus_tag="P5313_13905"
     CDS             440..1417
                     /locus_tag="P5313_13905"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRY81509.1"
                     /translation="MKLDDLASLLRTGDSYILTSHESPDGDGLGAMYALCRALEKLGK
                     KAAALLSEPLPPKYRFLDERGVFRVAETPPAADLDPASSLLLILDTNDAHYAGRIADT
                     LLDVVRGHVLIDHHESRGLDSSGHFLDSDASSTCEITFGLIRKLGVSLDPDMAVALFT
                     GIVYDTGSFGYPKTGENTFACALELVRAGVKPYSVHNLLYESSSVASLLLRKLVLSTL
                     ELHADNRIAVQIMRAVHLDDSGAVYEDSEDLINVPLRSETVQVSVFFKENKTGILRCS
                     LRSKGSVNVAHIAQGFGGGGHRTAAGFKCSLPLEQARKSVLDKLQDALP"
     gene            1433..2959
                     /locus_tag="P5313_13910"
     CDS             1433..2959
                     /locus_tag="P5313_13910"
                     /inference="COORDINATES: protein motif:HMM:NF033751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pallilysin-related adhesin"
                     /protein_id="HRY81510.1"
                     /translation="MKRATQILFLLCSIAAAGYIVFLAVDARSSRSQRDDKPMLARSV
                     AAEPRTEEGGAEGASEERARTVSEIPTASNEILLDVYSFNLDSDQEDEQILVVRRTDD
                     PSGLLRLIVADYLPGGRSWVRAWEGSTAVTKVRTLQILVKDVVGDHIPNIIAVGMNDR
                     GEQAVSIFWRTPPSREYRSPLFYTKICEVSGDSVTIEETERPESYKLGQTNAETWPVT
                     VHHRDPESENFLDQIRETWEWNFTRKAYERVLVERIPGAQIERQLVTRILDGTVETFQ
                     RYLEGIWYRASADPRAPGSQFVIFDPREKTLSFFSDNVMEPYQWEDSHPTRYGIFIGS
                     RSSAVKTLRRLIDVELTGHEAVSLRIFQDIRLKADVTEQWNGTYRKMNSELASSFRKV
                     TPVTQPRSAPLSGRYRGLDGSTLSFDPPRFTAARDGRSEAGGFSVFTLKGAEILEMKY
                     LTETGVVGKRVSYRVDRSQRKENGETVEILNLTPVRVAIDGIEFVEGSPLILERLPNP
                     "
     gene            complement(2962..4128)
                     /locus_tag="P5313_13915"
     CDS             complement(2962..4128)
                     /locus_tag="P5313_13915"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRY81511.1"
                     /translation="MIPDAVARVYSDRAVQGRRRGPVVPSAAALILALAAAFHAVPGA
                     VAQQPGSLRAELAGQTSLQDFPARLAAAADRASGLTELLALLEEFLPKVKEGPERRAL
                     FLRWAAVLELAGRWEEAAARYEEAAFAAPGQRDTESLLWAARAWLAAGETEKALSILR
                     VLGAASPEAAVRTRSTVLEGWARLIEGSAAEARTLAARAAASPPDRETLLSALTLLWA
                     ASEGPSRGEAAARIRREFPGTPEAVMVESGAVPPASHWLLTPAGGGARPSEAPAPGSP
                     AAAPPAATGSAPAKVLPPAPASSPAAASDPGPAAVRHSDSSRIASFQTGAFSEERNAD
                     ALVKELSGKGFAAVVERRERGGKPLWVVLVPAGADSQATLLRLKDAGYEAYPVF"
     gene            complement(4125..4430)
                     /locus_tag="P5313_13920"
     CDS             complement(4125..4430)
                     /locus_tag="P5313_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81512.1"
                     /translation="MPFRLFFLAVVIVLLVVFIGFNIENRFNVSFVFHTLEDVPVVVT
                     ILSSFALGLVAALPFALRRRNRSVVPKTPRGRRGKAGTAGGEAPRDERFPDGGAGVP"
     gene            complement(4430..5752)
                     /gene="miaB"
                     /locus_tag="P5313_13925"
     CDS             complement(4430..5752)
                     /gene="miaB"
                     /locus_tag="P5313_13925"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRY81513.1"
                     /translation="MKYYIETYGCQMNKAESSALENLFRERGWAPGEEDESDLVLINT
                     CSVRITAEDRAWGRIAHHAARKRTRPFTLVVTGCMAERLREEMRTAQPAVDYVIGNFQ
                     KQAFSDLIDAAGSGGRLEDVPEIPAFSFAPDHGTPGDFRAFVPIMHGCDNFCSYCIVP
                     YVRGREVSRPPGEILAEIDRLEDSGIREITLLGQNVNSYKWTDGEGGALDFPGLLRGI
                     SARLSERGRGGIRWIRFLSSHPKDLSDEALDAMTADERYCRHIHLCLQHGSDSILAAM
                     NRRYTAASYLALVDRIRSRMPDISLSTDILVGFPGETEEDLEATLTVMRKVGYSYSFM
                     YHFNPREGTPAARMENRVPDRVKKARLARVIALQKDLTRSRMEERLGSVTEVLVESVS
                     RKRRTELLGRSAGDMMVVFPGSPERVGTFARVLLESIQGNTFRAKEVP"
     gene            complement(5790..6146)
                     /locus_tag="P5313_13930"
     CDS             complement(5790..6146)
                     /locus_tag="P5313_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81514.1"
                     /translation="MSDQYPADPTELRNQGTRGVISTGAGLGLWVVNGLLRIPFVGAA
                     LGGVLVVLGILGLVGRNRTDRTTGVVLMAAGGAGLASLILPGLSSFLFGVGGLALVGF
                     GLFNLYKFLRGLKSRS"
     gene            complement(6334..>7314)
                     /locus_tag="P5313_13935"
     CDS             complement(6334..>7314)
                     /locus_tag="P5313_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HRY81515.1"
                     /translation="LQFGKHPGKTNLTDVDSALAILRYLTETPACAVMKHNNPSGAAR
                     ADTVEEAYRKAFEADRLAAFGGAVVLNRPADPRTARAIADSFCEVVAAPDFEPGAFDL
                     LAGRKNLRILRIPGLDRLSRYAPARNLDFRTLLDGGCVVQLSFVSRIRSAADLLPAEC
                     EHRGRSHSVARRPTEREVEDMLFGWAVETGVTSNSVIYVKDGVTIGIGTGEQDRVGVA
                     EIARDKAYRKLEDLLAWRRTSKPWNELDDPALREEIRREVEVARGGLAGSVMVSDAFF
                     PFRDGIEVGLREGVSAVLQPGGSLRDYESIEACNEYGAAMMFTGQRCFRH"
BASE COUNT         1232 a         2510 c         2469 g         1103 t
ORIGIN      
        1 cccggtacca atccgcggac tatacgatag cccaccgcta cctgacctac gtcaaccgcc
       61 gggatttcgg gcccttccgg gccattacca tcggattggg gctggatgac cggatcgccg
      121 cgaccgcgga gtccctggcc tccttcctcc ggaaccctcc ggagcggacg gaccggcccc
      181 ttcccggttc gccggagtcc ggagccgcgg gttccggacc cgggcaaggc gccgcggccc
      241 ccggttccgg ggcggatccc gacgcctccg gggctccggg cgccggtccg gacgcccgga
      301 acgccgcttc ggcgggcggt atcccctgga tcctggccgg actgggtgcc ggggttgccg
      361 cgctggcgat cctcgttctg gtcgtgcggg cgcgggcggg caaggaagaa cgcaagaaaa
      421 agcccgagga accgggggca tgaagctcga cgacctggcc tccctgcttc ggacggggga
      481 ttcctacatc ctgacgtccc acgaatcccc cgacggggac gggctgggcg cgatgtacgc
      541 cctctgcagg gccctggaaa aactcggcaa gaaggccgcg gccctgctct cggaacccct
      601 gcccccgaaa tacaggttcc tggacgagcg gggagtgttc cgggtggccg agacgcctcc
      661 tgcggcggac ctggacccgg cttccagcct acttcttatc ctggacacga acgacgcgca
      721 ttacgccggc cgcatcgcgg ataccctcct ggacgtcgtc cgcggccacg tcctgatcga
      781 ccaccacgaa tcccggggcc tggactcctc ggggcacttc ctggactccg acgcatcctc
      841 cacctgcgag atcaccttcg ggctgatccg caagcttggg gtttccctgg acccggacat
      901 ggccgtggcc ctgttcacgg gcatcgtgta cgataccggt tccttcggct atcccaagac
      961 cggagagaac accttcgcct gcgccctgga actggtccgg gccggggtga agccctattc
     1021 ggtgcacaac ctgctgtacg aaagctcctc cgtcgcctcc ctgctcctgc gcaagctggt
     1081 cctttcgacc ctggagctcc atgcggacaa ccgaatcgcc gtccagatca tgcgggcagt
     1141 ccatctggac gactccgggg cggtctacga ggactccgag gacctgatca acgtccccct
     1201 ccggagcgag accgtccagg tttccgtgtt cttcaaggag aacaagaccg gaatcctgcg
     1261 gtgttccctg cgatccaagg gatccgtgaa cgtggcccac atagcccagg gcttcggggg
     1321 aggcggccac cgcaccgccg cggggttcaa atgctccctt ccccttgaac aggcccggaa
     1381 atccgtgcta gacaagcttc aggatgctct tccctaggaa accgaaggcc ggatgaagcg
     1441 agccacccag atactcttcc ttctctgttc gatagccgcg gcggggtata tcgtcttcct
     1501 ggccgtcgac gcccggagca gccgatccca gcgggacgac aagccgatgc tcgcccggag
     1561 cgtggccgcg gagccccgca ccgaggaggg aggggccgaa ggcgcttcgg aggagcgggc
     1621 ccggacggtc agcgagatcc cgacagcctc caacgagatc ctgttggacg tctattcctt
     1681 caacctggat tccgaccagg aggacgagca gatcctggtg gtccgacgca ccgacgatcc
     1741 ctcgggcctg ctccggctga tcgtcgcgga ctatctgccc ggcggccgtt cctgggtacg
     1801 ggcctgggag ggttccaccg cggtcaccaa ggttaggacc ctccagatcc tcgtcaagga
     1861 cgtggtgggg gaccacatcc ccaacatcat cgccgtgggc atgaacgacc ggggagagca
     1921 ggccgtctcg atcttctggc ggaccccgcc ctcccgggaa taccgatccc ccctcttcta
     1981 caccaagatc tgcgaggttt ccggggattc cgtgaccatc gaggaaacgg agcgtccgga
     2041 aagctacaag ctcggacaga cgaacgcgga aacctggccc gtcacggtcc accaccggga
     2101 tccggaatcc gagaatttcc tggaccaaat ccgggaaacc tgggaatgga acttcacccg
     2161 gaaagcctac gagcgggtcc tggtggagcg gatcccgggc gcccagatcg aacgacagct
     2221 ggtcacccgg atcctggacg gcaccgtgga gaccttccag cggtacctgg agggcatctg
     2281 gtaccgggct tccgcggatc cccgggctcc gggttcccag ttcgtcatct tcgacccccg
     2341 ggagaagacc ctctcgttct tcagcgacaa tgtgatggaa ccctaccagt gggaggacag
     2401 ccaccccacc cgctacggga tcttcatcgg aagccggagc tccgcggtca agaccctgcg
     2461 tcggctgatc gacgtggagc tcacgggcca tgaagccgtt tccctcagga ttttccagga
     2521 catccgcctc aaggcggacg tgacggagca atggaacggg acctaccgga agatgaattc
     2581 cgagctggcc tcctccttcc gcaaggtgac ccccgtaacc cagccccgct ccgcccccct
     2641 ttcgggtagg taccggggcc tggacggctc caccctgtcc ttcgacccgc cccggttcac
     2701 ggcagcccgg gacggccgct ccgaggccgg ggggttctcg gtcttcaccc tcaagggcgc
     2761 ggaaatcctg gagatgaagt acctgaccga gacgggcgtc gtcggcaaga gagtctccta
     2821 ccgggtagac cgatcccagc ggaaggagaa cggggaaacc gtggagatcc tgaacctgac
     2881 acccgtgcgg gtggccatcg acgggatcga attcgtggaa gggtcccccc tgatcctcga
     2941 gcgccttccg aatccctgaa cttagaagac cggatacgcc tcgtagcccg cgtccttcag
     3001 acgcaggagg gtcgcctggg aatccgctcc cgcgggcacc aggacgaccc agaggggctt
     3061 gcctccccgc tccctccgct cgacgaccgc ggcgaaaccc ttgcccgaaa gttccttgac
     3121 cagggcgtcg gcgttccgtt cctcggagaa cgctcccgtc tggaacgacg cgatccggga
     3181 cgaatccgag tgccggacgg cggccgggcc ggggtcggac gcggcggcgg gggatgaagc
     3241 cggagcgggg ggcaagacct tagcgggagc cgatcccgtc gccgcgggcg gggccgcggc
     3301 gggactgccc ggggccggag cctcggaagg ccgggctccg ccgcccgcgg gggtcaggag
     3361 ccaatgggag gcagggggaa cggctccgct ctcgaccatg acggcttccg gagtgccggg
     3421 gaattccctg cggatccgcg cggcggcctc tccccgggag ggcccctccg aggcggccca
     3481 caggagggtc agggcggaca gcaaggtttc gcggtccggg ggcgaggctg cggcccgggc
     3541 ggccagggtt cgggcctccg cggcggatcc ttcgatcaac cgcgcccagc cctccaggac
     3601 ggtggaacgg gtccggaccg ccgcctccgg cgaggcggcc cccaggaccc ggaggatgga
     3661 cagggccttc tccgtctccc ccgcggccag ccaggcccgg gccgcccaca agaggctttc
     3721 cgtatcccgc tgcccggggg ccgcgaaggc ggcctcctcg taccgggccg ccgcttcctc
     3781 ccagcggccc gcgagctcca ggaccgcggc ccaacgcagg aacagggccc ggcgctccgg
     3841 cccctccttc accttgggca ggaactcctc cagcagggcc agaagctcgg taagcccgga
     3901 ggcccggtcg gcggccgcgg cgaggcgcgc gggaaaatcc tggagggatg tctggccggc
     3961 gagctcggcg cggagcgagc cgggctgctg cgcgaccgcc ccgggcaccg catggaaggc
     4021 agcggccagg gcgaggatga gggcggcggc ggaaggaacc acggggcccc ggcgacgccc
     4081 ctggacggct cgatccgaat atacccgggc aaccgcgtcc gggatcacgg aacccccgcg
     4141 cctccgtccg ggaaccgttc gtcccgggga gcctccccgc ccgcggtacc ggccttgccc
     4201 ctgcgccccc gcggggtctt gggtacgacc gagcggttcc gtcggcgcag ggcgaaggga
     4261 agggcggcga ccagacccag ggcgaaggag gagaggatgg tgaccaccac cgggacatcc
     4321 tccaaggtat ggaagacgaa ggagacattg aagcggttct cgatgttgaa tccgatgaag
     4381 accacgagaa ggacgatgac tacggccagg aaaaagaggc ggaagggcat cagggtacct
     4441 ccttggcgcg gaacgtgttc ccctggatag attctagcag aacccgggcg aaggtgccca
     4501 ccctttcggg actgcccggg aacacgacca tcatgtcccc cgcggaccgt ccgagtagct
     4561 cggtcctgcg cttgcgggac acggactcca ccaggacttc cgtgacggaa cccaggcgct
     4621 cctccatccg ggaccgggtc aggtccttct gcagggcgat gacccgggcc agacgggcct
     4681 tcttcacccg gtccgggacc cggttttcca tgcgggcggc gggggttccc tcccggggat
     4741 tgaagtggta catgaaggaa taggagtatc ccaccttccg catcaccgtc agggtcgcct
     4801 ccaggtcctc ctccgtctcc cccgggaacc ctacgaggat gtccgtggac agacttatgt
     4861 ccggcatccg ggaccgtatc cggtcgacca gggccaggta ggaagcggcc gtataccggc
     4921 ggttcatggc ggcaaggatg gaatccgagc cgtgctggag gcagaggtgg atgtgacggc
     4981 agtaccgttc gtccgcggtc atggcgtcca gggcctcgtc ggagaggtcc ttagggtggc
     5041 tggacaagaa ccggatccag cggatccccc cgcggccgcg ctccgagagc cgggcggaga
     5101 ttccccgcag gaggcccgga aaatcgaggg caccgccctc cccgtccgtc catttgtagg
     5161 aattcacgtt ctgccccagg agggtgatct cccggatgcc ggagtcctcc aggcggtcga
     5221 tctcagccag gatctccccg ggcggccggg acacctcccg gccccgcacg tagggaacga
     5281 tgcagtagct gcagaaattg tcgcagccgt gcatgatggg cacgaaggcc cggaagtcac
     5341 cgggggtccc gtgatcgggg gcgaaggaaa aggccgggat ctccggcacg tcctcgagcc
     5401 gtccgcccga gcccgcggcg tcgatgaggt ccgaaaaggc ctgtttctgg aagttgccga
     5461 ttacgtagtc gacggcgggc tgggcggtcc gcatctcctc ccggaggcgc tccgccatgc
     5521 agcccgtgac gaccagggtg aagggacgtg tccgtttccg ggcggcatga tgggctatac
     5581 ggccccaggc ccggtcctcg gcggtgatcc ggacggagca ggtgttgatg aggaccaggt
     5641 ccgactcgtc ctcctccccc ggagcccacc cgcgctcgcg gaagaggttc tccagggcgg
     5701 aggactcggc cttgttcatc tggcagccgt aggtttcgat gtagtacttc atggggagtc
     5761 gggtttccta cggcggccgc tcaggcccgt caagaccggg acttgagtcc cctcaggaat
     5821 ttgtacaggt tgaagaggcc gaagcccacc agggccagac cgccgacccc gaacaggaag
     5881 gagctgagcc cgggaaggat cagggaggcc agaccggctc cgccggcggc catgagtacg
     5941 acaccggtgg tccggtcggt ccgattccgg ccgacgagtc cgaggatgcc caggaccacg
     6001 agaacgccgc ccagggcggc ccccacgaag ggaatgcgca aaagtccgtt cacgacccac
     6061 aggcccagac ccgccccggt cgagatgact ccccgggtgc cctggttccg gagttccgtg
     6121 gggtccgcgg gatactgatc gctcatcttc tttctcctcc ttcgccgccg gcggacggcg
     6181 gccgtcaccc cgatggcgtg gcgtaccgag gcgtcttccg gctcgacatg agattaagat
     6241 atcgaaacgg accggcgggg taaagccttc cggacccgcg gggacgcaaa aacgcggggc
     6301 tgtccgatac gtgaccgacc attgcacacc ccgtcaatgc cggaagcagc gctggccggt
     6361 gaacatcatg gccgccccgt actcgttgca ggcctcgatg ctctcgtagt cccgcaggga
     6421 gcccccgggc tggaggaccg cggatacccc ctcccggagt cccacctcga tcccgtcccg
     6481 gaaggggaag aatgcgtcgg agaccatgac ggatccggcc agccccccgc gggcgacctc
     6541 cacctcccgg cggatctcct cccggagcgc cgggtcgtcc agttcgttcc agggcttgct
     6601 ggtcctgcgc caggccagaa ggtcctcgag cttgcgatag gccttgtccc gggcgatctc
     6661 ggccaccccc acccggtcct gttcccccgt gccgatcccg atggtgacgc cgtccttgac
     6721 gtagatgacc gagttgctgg tcaccccggt ctccacggcc cagccgaaga gcatgtcctc
     6781 gacctcccgt tccgtgggcc gtcgggccac ggagtgggag cggcccctgt gctcgcactc
     6841 cgcgggcagg aggtccgcgg cggaccggat ccgggatacg aaggagagct ggacgacgca
     6901 gcccccgtcc aggagggtcc ggaaatccag gttccgggcc ggggcgtagc ggctcagccg
     6961 gtcgagcccg gggatccgga ggatgcgcag gttcttccgg cccgccagca ggtcgaaggc
     7021 tccgggctcg aagtccggtg ccgccacgac ctcgcagaag gagtccgcga tagcccgggc
     7081 ggtccgggga tccgcggggc ggttcaggac cacggccccc ccgaaggcgg ccaggcggtc
     7141 cgcctcgaag gccttccgat aggcctcctc caccgtgtcc gcccgggcgg ccccggaggg
     7201 gttgttgtgc ttcatcaccg cgcaggcggg ggtttccgtc aggtaccgga ggatggccag
     7261 ggcggaatca acgtcggtca ggttggtctt gccggggtgc ttgccgaact ggag
//
LOCUS       DAOTEI010000174         7288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7263_length_7288_cov_4.580948, whole genome shotgun sequence.
ACCESSION   DAOTEI010000174 DAOTEI010000000
VERSION     DAOTEI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7263_length_7288_cov_4.580948"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="P5313_13940"
     CDS             complement(<1..1167)
                     /locus_tag="P5313_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY81516.1"
                     /translation="MKKVSVRAAIILSILAALSACVSSPPPAEEPVPAAPAEPDVAAL
                     ARAGDLAAVRDLGKGRALVNKPGANGDYPLHAAAVRAEPEMAALLLEQGADLESRDAA
                     GKTPLRRALEAGTLKTAEFLASRGADLFAADSAGATPLDDALAKGPDPLAAVVQAANV
                     DRKGADGRTALHAAADRLLVPAVTYLLTLSPDLSIRDNAGRTALDCALLHPSRKESAQ
                     IARLLVLRGAVPGFGDFEWFILAARSGDWARPRFSEGDTVLHRAVRNNLKGFAEYFLD
                     EKVPVDLKNAAGATSLHEAVRAGRYDIAELLLARGANPNARDGFGNTALHIALPESGR
                     RDGVELLVRYKADPSLKDRSGNTPLHVAVQLGYGADTAPLFLDAGAFVDSANAEG"
     gene            1335..2315
                     /locus_tag="P5313_13945"
     CDS             1335..2315
                     /locus_tag="P5313_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRY81517.1"
                     /translation="MKIGFCLPLNQHWRDNIERLARAFPGVEFVADPDRSLAEFESLD
                     AVVANPVPREKYEGARNLKALFVPFVGINHLPADLLLDRGVEVFNCHGNAFSVAERAL
                     AMTLAAFGRIIEFHNDLRQGIWHGFWVGKGHEDFWHSLQGKTCAVLGTGAIGTALAKL
                     LSAFDCRVIGYRRRPGAGVPPHFDRVTADLDEAVRDAELVFVALPLTSETEGLLSRRI
                     LESMKGKFLVNVGRGGIVDEEGLYRALKDGILAGAAIDTWYTYPPKGETAGAPSRFPI
                     HELPNVVLSPHVAGSTWEAVANNAVQTVDNVAEWLRTGSCGSKVDLRASY"
     gene            complement(2320..4128)
                     /locus_tag="P5313_13950"
     CDS             complement(2320..4128)
                     /locus_tag="P5313_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81518.1"
                     /translation="MRILFIRLPAPDPSRQALREAVPLAAARAAALAESRGLLDRGDW
                     SFLDRNVTDYGGDAAVVAAAAASGARAVLFELEPHNLDRSAWIAKRLRTRSPRVLLAA
                     HGPEARRDMPIFRASTFDAVLEGEPEESFCSFLTDLAAGSARPHYLSEPSREFGAWPD
                     PYLAGTLAVEPDRPVFIETVRGCPPPCLDAPGSGGSLRFRPEDQAARIVRLASKNGAP
                     ELRFQDKALSLRPDLPKFFREAAGANDAGVPIELELDPGILTEELGGLMADAAVETVR
                     TGPWSTNPAALAALGTAWNREEFERGCGILTSLGIRVRPSLLLGLPFDDYDAVIDSFD
                     FLGMAGLGQDALVSPLSVLPGTRIRDRAEDLGIKEYLRRPPYYVMETEWIDEDGIADA
                     AAAFEESFDVALAPPVAPAFEETIRGFLSFADLRKPGSLDRLLVNPETLGNSLTLLLS
                     GDDEEGIQRLARAARDLRKENPFGLYQIVLYSDKRIPGEAVQRKIADAFIEPDHWYEL
                     SRIYSLDPQRSFQARVFFATRKPSLALAALREAQDLETIFVLDAEPARGWERVFDALP
                     FLAFDRDSVPFGLLYDAMNAYRAYPDLLIEARAEVW"
     gene            complement(4203..4970)
                     /gene="aat"
                     /locus_tag="P5313_13955"
     CDS             complement(4203..4970)
                     /gene="aat"
                     /locus_tag="P5313_13955"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRY81519.1"
                     /translation="MSDGTPAPSEVPFLSEDERIEFPDPRSADRRGILCVGANLSPGV
                     LLSAYSRGIFPWFSEGDPILWWSPNPRFVLFPPEIRVSESMRKVLRKGTYTLTLDRDF
                     RGVIEACSSAPRPGQDGTWITRDMIEAYVRLHHLGFAHSVEAWKGSELAGGLYGVSLG
                     RLFCGESMFSRAPNASKAALIALAWRLADEGFPVIDSQVRTEHVASMGGRNIPRTRYL
                     DLVRACLELPGLRGSWDGLLAGFPDSAGWRKGVLRRS"
     gene            complement(4967..5977)
                     /locus_tag="P5313_13960"
     CDS             complement(4967..5977)
                     /locus_tag="P5313_13960"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRY81520.1"
                     /translation="MIGYERDTLREGPLLRPMDVPGLAALAFFIVASILVPHTGTTQA
                     GPWTMPRGYAVAAGYGAAAALILALAAAERRYGGRILCFLRTLYPLLLITPLFLDSIL
                     LSAQIFGGRSHDAFFRALDEAIFGFEPYLEFYKSFESFPRFNELMFGSYFTYYVMIAT
                     VLWIPWIKGDREETERAVFTYFMIQLIVAVWYVFFRVQGPKYWVPELREHWYGNFVGF
                     FFVPFFQRGFETVTLSGAAFPSSHVLFTTLCVLFAWRWDRRLLAFYLPMLALVLLATV
                     YIFAHYAVDGLAALVLAPALYAFSFRLYDPVRKLLGYQVRSGRAVRGAAARAAANAAE
                     GT"
     gene            complement(6009..>7288)
                     /locus_tag="P5313_13965"
     CDS             complement(6009..>7288)
                     /locus_tag="P5313_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY81521.1"
                     /translation="FFEKDRALSRRFQKIDIVEPSLAETSEILKGLRPKYEEYHKVRY
                     SDEALDLAVRLSAQYVTERRLPDKAIDVMDEAGAWARIASFKRSGADQPEEDVLDIGA
                     REIETVVAKIARIPEKTVSGSEKEKLAGLEEDLKKVVFGQDPAVEAVAKAVKRSRAGF
                     RHPDKPVANFLFVGPTGVGKTELARRLAERLGVALHRFDMSEYQEKHTVSRLIGSPPG
                     YVGYEEGGLLTDAIRKTPHAVVLMDEIEKAHPDVYNILLSIMDYATLTDNQGRKADFR
                     NVVLIMTSNAGAREIGKSMIGFGERAVTDSALDDAVQKTFTPEFRNRLDAVVRFGHLP
                     LEIIERIVVKAVDDFRVQLAEKKVELEMTDACVKLLAEKGYSKEFGARNVGRLVEDKV
                     KTWFVDEVLFGRLEKGGKVLADAVDGEVVLRTL"
BASE COUNT         1164 a         2418 c         2591 g         1115 t
ORIGIN      
        1 cccctccgcg ttggcggaat ccacgaaggc tccggcgtcc aggaagaggg gggccgtgtc
       61 cgccccgtag cccagctgga cggccacgtg gaggggggtg ttcccgctcc ggtccttcag
      121 ggaggggtcc gccttgtacc ggacgagaag ctcgaccccg tcccgccgcc ccgactcggg
      181 aagggcgatg tgcagggccg tgttcccgaa accgtcccgg gcgttcgggt tggccccccg
      241 ggccaggagg agctccgcga tgtcgtagcg gccggcccgg accgcctcgt gcaggctggt
      301 ggcccccgcg gcgttcttga ggtccaccgg gaccttctcg tccaggaagt attccgcgaa
      361 gcctttcagg ttgttccgca ccgcccggtg gaggaccgta tccccctccg agaaccgcgg
      421 ccgggcccag tcgccggacc gggccgccag gatgaaccac tcgaagtccc cgaacccggg
      481 gacggctccc cgcaggacta agagtcgggc gatctgggcg ctctccttgc gggaggggtg
      541 caggagggca cagtccaggg ccgtgcgccc ggcgttgtcc cggatggaca ggtccgggga
      601 gagggtgagc aggtaggtaa ccgccggtac caggagccgg tccgcggcgg cgtgcagggc
      661 ggtccgcccg tccgcgccct tccggtcgac gttggccgcc tggacgacgg cggccagggg
      721 atccggtccc ttggccaggg cgtcgtccag gggggtggcg cccgcggagt ccgccgcgaa
      781 gaggtccgcc ccccgggagg ccaggaactc ggcggtcttg agggtgccgg cctccagggc
      841 tctccgcagg ggggtcttcc cggccgcgtc ccggctctcg aggtcggcgc cctgttccag
      901 gaggagggcc gccatctccg gttccgcccg gacggcggcc gcatggaggg gatagtcccc
      961 gttcgccccg ggcttgttga ccagggcccg gcccttgccc aggtcccgga ccgcggcgag
     1021 gtctcccgcc cgggccaggg ccgccacgtc cggttccgcg ggtgccgcgg gaacgggctc
     1081 ttccgcggga gggggggagg aaacgcaggc ggatagcgcc gcgaggatcg aaagaatgat
     1141 agccgcccga acggatactt ttttcatcac cgcccctccg aggcccgggc cgggatcgaa
     1201 cgcgtcgtcc gcctagccgg gcatgttcta ctgggtagga tagtaccgca ccgagggcga
     1261 atcgtcgacc gggttgccgc ggttcccttc cgtttggtac tttctatcac aagcccatca
     1321 ctccggagga gcccgtgaag atcggattct gcctgccctt gaaccagcac tggagagaca
     1381 acatcgaacg cctggcccga gcctttcccg gcgtcgagtt cgtggcggat cccgaccggt
     1441 ccctggcgga gttcgagagc ctggacgccg tggtggccaa tccggtcccc cgggagaagt
     1501 acgagggggc gcggaacctg aaggccctct tcgtgccctt cgtggggatc aaccacctgc
     1561 ccgccgacct cctcctggac cggggggtgg aggtgttcaa ctgccacggg aacgccttca
     1621 gcgtggccga gcgggccctg gccatgaccc tggccgcctt cggccggatc atcgagttcc
     1681 acaacgatct ccgtcaaggc atctggcacg gcttctgggt cggcaaagga cacgaggact
     1741 tctggcattc cctgcaggga aagacctgcg cggtcctggg cacgggggcg atcgggaccg
     1801 ccctggccaa gctcctttcg gccttcgact gccgggtcat cgggtaccgc cggagacccg
     1861 gcgccggggt ccccccccat ttcgaccggg tcaccgccga cctggacgag gccgtccggg
     1921 acgcagagct cgtgttcgta gccctgccct tgacctccga gaccgagggg ctcctgtccc
     1981 ggcgtatcct ggagtccatg aagggcaagt tcctggtgaa cgtggggcgg ggcgggatcg
     2041 tggacgagga ggggctctac cgggccttga aggacggtat cctcgccgga gccgccatcg
     2101 acacctggta tacctatcct cccaagggcg agaccgccgg ggctccctcc cgcttcccga
     2161 tccacgaact gcccaacgtg gtcctctccc cccacgttgc cggctccacc tgggaggctg
     2221 tggcgaacaa tgcagtccag accgtggaca acgtggccga gtggctgcgc acgggaagct
     2281 gcggctcgaa ggtggacctg cgggcgagtt actgaacggt caccacactt ccgccctcgc
     2341 ctcgatcagc aagtccgggt aggcccggta ggcgttcatg gcgtcgtaca agaggccgaa
     2401 gggtacggag tcccggtcga aggccaggaa gggcagggcg tcgaagaccc gctcccagcc
     2461 ccgggcgggc tcggcgtcca ggacgaagat ggtctccagg tcctgggctt cccgcagggc
     2521 ggccagggcc agggagggct tgcgggtagc gaagaagacc cgggcctgga aggatctctg
     2581 gggatccagg gaatagatcc gggagagttc gtaccagtgg tccggttcaa tgaaggcgtc
     2641 cgcgatcttc cgctggacgg cctccccggg gatccgcttg tccgagtaga ggacgatctg
     2701 gtagagcccg aagggattct ccttgcgcag gtcccgggcg gcccgggcca ggcgctggat
     2761 tccctcctcg tcgtccccgg acagaaggag ggtcagggaa ttgcccaggg tttccggatt
     2821 gaccaggagc ctgtccagac tgccgggctt gcgcaggtcc gcgaaggaca ggaaaccccg
     2881 gatcgtctcc tcgaaggccg gggccaccgg gggcgccagg gccacgtcga agctctcctc
     2941 gaaggccgcg gcggcgtctg cgatgccgtc ctcgtcgatc cactcggtct ccatcacgta
     3001 gtacggaggc cggcgcaggt attccttgat tcccaggtcc tcggcccggt cccggatgcg
     3061 ggtgcccggc aggactgaca ggggggacac cagggcgtcc tgccccaggc cggccatgcc
     3121 caggaagtcg aaggagtcga tgaccgcgtc gtagtcgtcg aagggcaggc ccaggagcag
     3181 ggagggacgg acccggattc ccagggaggt caggatcccg cagccccgct cgaactcctc
     3241 ccggttccag gcggtcccca gggccgcaag ggccgcggga ttcgtggacc aggggccggt
     3301 ccggacggtc tccaccgccg cgtccgccat gagtcccccg agctcctcgg tcaggatgcc
     3361 cggatcgagt tccagttcga tgggcacccc cgcgtcgttg gcccccgcgg cttcccggaa
     3421 gaacttgggc aggtccggcc tgagggacag ggccttgtcc tggaaccgaa gttccggggc
     3481 tccattcttg gaagccagcc gcacgatccg ggccgcctgg tcttccggac ggaaccggag
     3541 gctccccccc gagcccggag cgtccaggca gggcggcggg cagccccgga cggtctcgat
     3601 gaacacgggg cggtccggct ccaccgccag ggtacccgcc aggtaggggt ccggccaggc
     3661 tccgaactcc cgggatggct ccgagaggta atgggggcgg gcggagcccg cggcgaggtc
     3721 cgtcaggaag gagcagaagg attcctcggg ctccccctcc aggacggcgt cgaaggtcga
     3781 ggcccggaat atgggcatgt cccggcgggc ttccggtccg tgggcggcca ggaggacgcg
     3841 cggggaacgg gtccggaggc gcttggcgat ccaggcgctc cggtccaggt tgtggggctc
     3901 cagttcgaac aggacggccc gggcccccga ggccgcggcc gccgcgacga cggcggcgtc
     3961 gcccccgtaa tccgtcacgt tccggtccag gaaagaccag tccccccggt ccaggagtcc
     4021 ccgggattcc gccagggcgg cggcgcgggc ggcggccagg ggtacggcct ccctcagggc
     4081 ctggcgggag ggatcggggg cgggcagtcg gatgaacagg attctcacgg gatggaacct
     4141 cgggctacgg cggggccgga atcggtacgt tccagtatat cagaaaggga tccgtctgtc
     4201 tttcagctcc ggcggagaac ccccttccgc catccggcgg aatcggggaa cccggccagg
     4261 agcccgtccc aggacccccg caggcccgga agttccaggc aggcccggac cagatcgaga
     4321 taccgggtcc ggggtatgtt ccttcccccc atggacgcga cgtgctctgt acggacctgg
     4381 ctgtcgatca cggggaagcc ctcgtccgcc aggcgccaag ccagggcgat cagggccgcc
     4441 ttggaggcgt tcggcgccct ggagaacatg gactccccgc agaataggcg gcccaggctc
     4501 acgccgtaca gccctccggc cagttccgac cccttccagg cctccacgga gtgggcgaag
     4561 cccagatggt ggagacggac gtaggcctcg atcatgtccc gggtgatcca ggtgccgtcc
     4621 tggccgggcc ggggcgccga ggagcaggcc tcgatcaccc cccggaagtc ccgatccagg
     4681 gtcagggtgt acgtgccctt gcggagcacc ttgcgcatgg actcggagac gcggatctcc
     4741 ggggggaaga ggacgaaccg gggattcggg ctccaccaga ggatggggtc cccttcggag
     4801 aaccagggga agatcccccg cgaataggcg gacagaagga ccccggggga gaggttggcg
     4861 ccgacgcaga ggatcccccg ccggtccgcg ctccgggggt ccggaaactc gatccgctca
     4921 tcctcggaaa ggaagggaac ctccgagggg gccggggtgc cgtcgctcat gtcccttcgg
     4981 ccgcgttcgc ggcggccctc gcggcggcgc cccggaccgc ccggccggac cggacttgat
     5041 accccagaag ctttcgcacg gggtcgtaga gccggaagga gaaggcgtag agcgcggggg
     5101 ccaggactag ggccgccaga ccgtccacgg cgtagtgggc gaagatgtag accgtggcca
     5161 ggaggaccag ggccagcatg ggcaggtaga acgccaaaag gcgcctgtcc cagcgccagg
     5221 cgaagaggac gcagagggtg gtgaacagta cgtgggagga ggggaaggcg gcccccgaga
     5281 gggtcacggt ctcgaagccc cgctggaaga agggcacgaa gaagaaaccc acgaagttgc
     5341 cgtaccagtg ttcccggagc tcggggaccc agtacttcgg gccctggacc cggaaaaaga
     5401 cgtaccagac ggctacgatc agctgtatca tgaaatatgt gaagaccgct cgctcggttt
     5461 cctcccggtc gcccttgatc caggggatcc agagcaccgt ggcgatcatc acgtagtagg
     5521 tgaagtagga gccgaacatg agctcgttga acctgggaaa ggactcgaag gacttgtaga
     5581 actccaggta gggctcgaac ccgaagatcg cctcgtccag ggcgcggaag aaggcgtcgt
     5641 gggaccgtcc cccgaagatc tgggcggaca gcaggatgct gtccaggaac aaaggggtga
     5701 tcaggagcag gggatagagg gtgcggagga agcacaggat ccgtcccccg tacctgcgct
     5761 ccgcggcggc cagggccagg atgagggcgg ctgccgcccc gtagccggcc gccacggcgt
     5821 agccccgggg catcgtccag gggcccgcct gggtcgtgcc ggtatggggt acgaggatcg
     5881 aagcgacgat gaagaaggcc agggcggcca ggccggggac gtccatcggc cggagcaggg
     5941 gaccttcccg cagggtgtcc cgttcgtatc ctatcatggc ggtattccgg atggtttcgc
     6001 gcggggccct agagggtgcg caggacgact tccccgtcca ccgcgtccgc caggaccttc
     6061 ccgccctttt ccaggcgccc gaagagaacc tcgtccacga accaggtctt caccttgtcc
     6121 tcgaccaggc gtcccacgtt tcgggccccg aactccttgg aatatccctt ctcggccagg
     6181 agcttgacgc aggcgtccgt catctcgagc tcgaccttct tctccgcgag ctggacccgg
     6241 aaatcgtcca ccgccttcac gacgatccgc tcgatgatct ccaggggcag gtggccgaag
     6301 cggaccaccg cgtccaggcg gttgcggaac tccggggtga aggtcttctg taccgcgtcg
     6361 tccagggctg agtccgtcac ggcccgttcc ccgaatccga tcatggactt tccgatctcc
     6421 cgggcgcccg cgttgctggt catgatgagg acgacgttgc ggaaatccgc cttccggccc
     6481 tggttgtccg tgagggttgc atagtccatg atcgaaagga ggatgttgta gacgtccggg
     6541 tgggccttct cgatctcgtc catcagcacc acggcgtggg gggtcttgcg gatcgcgtcg
     6601 gtcaggaggc ccccctcctc gtagcccacg tacccggggg gggatccgat gaggcgggag
     6661 accgtgtgct tctcctggta ctcgctcatg tcgaaacggt gcagggccac acccaggcgc
     6721 tccgccagtc ggcgggccag ctcggtcttg ccgacccccg tgggtcccac gaagaggaag
     6781 ttggccaccg gcttgtcggg gtgccggaac ccggcccggc tgcgcttgac cgccttggcc
     6841 acggcctcca ccgccgggtc ctggccgaag accaccttct tgaggtcctc ctccaggccc
     6901 gccagcttct ccttctcgga gcccgagacg gtcttttccg ggatccgggc gatcttggcc
     6961 accacggtct cgatctcccg ggccccgatg tccaggacgt cctcctccgg ctggtcggcg
     7021 ccggacctct tgaaggatgc gatccgggcc caggccccgg cttcgtccat gacgtcgatg
     7081 gccttgtccg gcagccgccg ctccgtgacg tactgggcgg agaggcggac cgccaggtcc
     7141 agggcctcgt ccgagtaccg gaccttgtgg tactcctcgt acttgggccg gagtcccttg
     7201 aggatctcgg aggtttccgc cagggagggt tccacgatgt cgatcttctg gaagcgccgg
     7261 gagagggccc ggtccttctc gaagaact
//
LOCUS       DAOTEI010000175         7278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7278_length_7278_cov_6.389450, whole genome shotgun sequence.
ACCESSION   DAOTEI010000175 DAOTEI010000000
VERSION     DAOTEI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7278_length_7278_cov_6.389450"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="P5313_13970"
     CDS             <1..794
                     /locus_tag="P5313_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY81522.1"
                     /translation="FDSKHIGKVWITKEDWKRSLPPMLRSSPLGFIVGVLPGMGGTIA
                     TFMSYAMEKNLSKKPEEFGKGAIEGLAAPEAANNACSCGAMVPLLTLGIPGSGTTAVM
                     LGALMLLGVRPGPTLFTDHPEIAWGVIASMLIGNIILIIINLPLAIPLVQILKVPTRI
                     MLPIIMGLALIGTYFLNYSSFDFVVVMVSAVIGYLFSKLEIPLPPLVLALILGGTTEQ
                     ALRNSLVLSDGSLGIFVQKPISAVFIVLAAASLVYSLAKRKKHAA"
     gene            791..1696
                     /locus_tag="P5313_13975"
     CDS             791..1696
                     /locus_tag="P5313_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY81523.1"
                     /translation="MKPDRTAGRPRRSLLYIPGNNPGMIQNCSIYGADGVLLDLEDSI
                     ALAEKDAARKLVRGALRALDFGGVERVVRINGRDTPYFEKDLAEIVPARPDAVRLPKV
                     DGPEDVLEADRIISRLEKENGIEPGTVRIQAMLETARAIVNAAAIASSSPRLSGLTLG
                     GQDLAADLGIKATKDGRELLYAKSAVVIAAKASGLEAFDTVYTDVNDLEGLLEATRTA
                     VSLGFSGKAAIHPSQVPVIHVAFAPTESEVRKARAVVRAAREAEARGAGVVALDGRMI
                     DAPVVAQAERTLELARLAGMEDAQA"
     gene            1693..3258
                     /gene="citF"
                     /locus_tag="P5313_13980"
     CDS             1693..3258
                     /gene="citF"
                     /locus_tag="P5313_13980"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HRY81524.1"
                     /translation="MKLDCVTNSIGRRVPREIPGLGVLAPYAGAYSRLDGSYVYTPKT
                     FPRRVPAPTTDKVAPSLAAAIERSGLESGMTVSFHHHLRNGDTVVEQVLAAAEALGIR
                     NLTLAPSSLGDEADCVAGFIRRGVVSRLHTSGVRGEVGKAISRGEIEAPVVIRSHGGR
                     ARAVEEGSLRIDVAFLAAPACDRLGNMTGSAGKTACGSLGYAIHDARYAGRVVAVTDN
                     LVPYPLAPRISIPQYLVDHVAVVDSIGDPSKIATGATRISRDPVQLRIAELAFGLIKS
                     TGYLKDGCSFQTGGGGPSLAAARYVRDYMKEKGIRGSYCIGGITGFMAEMLKEGLFEA
                     LFDVQSFDAAVTSSMADNPRHMEADVSCYANPLNKGCLVNDLDVVVLAALDTDVDFNV
                     DVLTGHDGVLRGASGGHSDTAAGAKLAVITVPSMRNGVPSIRNRVQTVVTPGETIDAI
                     VTERGIAINPRREDLAAAARAAGLPIKDIRDLKAEIERVTGVPDEVEFADDIVGIVEY
                     RDGTIIDSIRRVV"
     gene            3292..3780
                     /locus_tag="P5313_13985"
     CDS             3292..3780
                     /locus_tag="P5313_13985"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRY81525.1"
                     /translation="MQQPWIIGAAVLAGAFLGDRLKLPSGILTGGMIAGLVAKGFVEG
                     NVPSGRALSVISQLLVAYVVVSNSDVATIRKHPEILPIAVGYIVALTLFCLGAAWAIN
                     KLFHIDLETAIYATAPGGLSGMALSATEAGAETPVSMMFHLLRLTLILVFTPFLAALF
                     GK"
     gene            complement(3811..4833)
                     /locus_tag="P5313_13990"
     CDS             complement(3811..4833)
                     /locus_tag="P5313_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY81526.1"
                     /translation="MRILFVGGTGNISAACTRRALSEGFEVFNLNRGNRPDRTAPGVV
                     TLTADIRNPETVKTALAGLRFDGVVQFLAYRPEHVRADLELFEGIAGQYVLISTCSAY
                     RKPSLSPVITEDTPLENPFWEYSRLKIACEAVLREEGTARSFPFTIVRPSHTYDDGWI
                     PGVFGSSDYTIPWRMRGGKAVVVPGDGQSLWTLTHASDFAVGLVGLLGNPAALSEAFH
                     ITSDERLTWDAIHAILAEALGVRAETVHIPSDYIARIRPERGAGLLGDKAVSVLFDNS
                     KIRSFVPEFRPEVTFAEGVRRSLEWYDARPDLKVPNPVVDAEIDEILGYWRKALALLP
                     KVDGKP"
     gene            4945..6051
                     /locus_tag="P5313_13995"
     CDS             4945..6051
                     /locus_tag="P5313_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRY81527.1"
                     /translation="MFEFSQDEVAEKKRLIRGLLEETGLTGIYLKKTANFSWVTAGGY
                     NIVGLSTELGVAGVLLTPDRGFVVCSNIEVPRMEQEERLVEQGYEIVGFPWYEDREAA
                     LVASLGGPRLGADHAFPGAVDVSGKINPLRYSLTSWEIARYRGQARETARAAEETALE
                     IRPGDEERAVVGRLSDRLWSRGMDFIVAFCAADDRISRYRHPVATDAKIRKRAMISVN
                     SRRRGLIVSITRFVQFGKVPGDLQRRYEDNVYVDSVMMAATKPGRPASEVFQAAVRAY
                     AEKGYPDEYKLHHQGGAIGYIGRDYKATERTTEIIRENQAFTWNPSITGAKSEDTMLA
                     SSSGPIVLSEPVIFPTILSEAGGFVFRRPGIAEL"
     gene            6091..7068
                     /locus_tag="P5313_14000"
     CDS             6091..7068
                     /locus_tag="P5313_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY81528.1"
                     /translation="MNVQRVFVATVLCVLAAVALTADGTKEAKFPEREIEVMVPWAAG
                     GATDVTFRAFTGVIQKYLGVPMIISNKPGGAAVPGYVEAMTKSPDGHYLVAWATPSIT
                     VTHMQNTPYDATTFEPVLNIVTAPVWFLVPANSPYKNLADLVADAKKRPGMINLGNAG
                     TGGGTHMIALAFETAAGAKFNHVPHAGGGPTVTAAVAGQVDVITVGPPEGVPQLASGQ
                     LRCLGVFSDKRLEQFPDYPTAKELGIPFSMGQWRGLAVPKGTDPKKIKILHDAFKAAM
                     ADPEFLAIAEKSGLLLDYRDTVSFRKLIQEQDLVYKTTVQTNKLGDKYK"
BASE COUNT         1236 a         2465 c         2385 g         1192 t
ORIGIN      
        1 agttcgactc caagcacatc ggcaaggtct ggatcaccaa ggaggactgg aagcgctccc
       61 tgcccccgat gctccgcagt tcccccctgg gcttcatcgt gggagtcctc ccgggcatgg
      121 gcggcaccat cgccaccttc atgtcctacg ccatggagaa gaacctgagc aagaagcccg
      181 aggagttcgg caagggcgcc atcgagggcc tggcggcccc ggaggcggcc aacaacgcct
      241 gctcctgcgg cgccatggtt cccctcctga ccttgggcat ccccggatcc gggaccaccg
      301 ccgtcatgtt gggcgctctg atgctcctgg gcgtacggcc cggtccgacc ctcttcacgg
      361 accaccccga gatcgcctgg ggcgtcatcg cctccatgct gatcgggaac atcatcctga
      421 tcatcatcaa cctccccctg gccattcccc tggtccagat cctcaaggtg cccacccgca
      481 tcatgctgcc catcatcatg gggctggccc tgatcgggac ttacttcctg aactacagct
      541 ccttcgactt cgtcgtggtg atggtctccg cggtgatcgg gtacctcttc tccaagctcg
      601 agatacccct gcctcccctg gtcctagccc tgatcctggg gggcaccacg gagcaggccc
      661 tgcggaactc cctggtcctg tccgacggct ccctgggcat cttcgtccag aagccgatct
      721 ccgcggtctt cattgtcctg gccgccgcct cgctggtata ctcgctggcc aagaggaaga
      781 agcacgcagc atgaaacccg acaggacggc ggggcgcccc cggcgctccc tgctgtacat
      841 ccccgggaac aacccgggga tgatccagaa ctgttcgatc tacggggcgg acggggtcct
      901 cctggacctg gaggactcca tcgccctggc ggagaaggac gcggcccgca agctggtccg
      961 cggcgccctc cgggccctcg acttcggggg cgtggagagg gtggtgcgga tcaacgggcg
     1021 ggacacgccc tacttcgaga aggacctcgc ggaaatcgtc ccggcccgtc cggacgccgt
     1081 ccgccttccc aaggtggacg gccccgagga cgtgctcgag gcggaccgga tcatctcccg
     1141 cctggagaag gagaacggga tcgagcccgg gacggtccgg atccaggcca tgctggagac
     1201 cgcccgggcc atagtgaacg ccgcggccat agcctcctcc tctccccggc tctcggggct
     1261 gaccctgggc ggccaggacc tggccgcgga cctggggatc aaggccacca aggacggccg
     1321 ggaacttctg tacgccaaga gcgccgtggt catcgccgcc aaggcttcgg gcctggaggc
     1381 cttcgacacg gtctacacgg acgtgaacga cctggagggc ctcctggagg ccacccggac
     1441 ggcggtgtcc ctgggcttct cgggcaaggc cgccatccat ccctcccagg tcccggtgat
     1501 ccacgtggcc ttcgctccga ccgagtccga ggtccgcaag gcccgggccg tggtccgggc
     1561 ggcccgggag gcggaagccc ggggcgcggg ggtggtggcc ctggacgggc gcatgatcga
     1621 cgcccccgtc gtggcccagg cggagcggac cctggagctc gcccgcctcg ccggcatgga
     1681 ggacgcccaa gcatgaagct cgactgcgtg accaattcca tcggacggcg ggtgccccgg
     1741 gagatcccgg ggctcggcgt cctggccccc tacgcgggag cctactcccg gttggacggt
     1801 tcctacgtct acaccccgaa gaccttcccg cgccgggttc ccgctcccac caccgacaag
     1861 gtcgcgccca gcctggccgc cgcgatcgag cgctcgggcc tggaaagcgg catgaccgtc
     1921 tccttccacc accacctgcg caacggcgat accgtggtgg agcaggtcct ggccgctgcg
     1981 gaggccctgg ggatccggaa cctgacactg gctcccagtt ccctggggga cgaggcggac
     2041 tgcgtggccg gcttcatccg gcggggcgtc gtgtcccgcc tgcacacctc cggggttcgt
     2101 ggagaggtcg gcaaagccat ctcccggggc gagatcgagg ccccggtggt catccggagc
     2161 cacgggggac gtgcccgggc cgtggaggag gggagcctgc ggatcgacgt ggccttcctg
     2221 gccgccccgg cctgcgaccg gctcggcaac atgaccggtt ccgcgggcaa gaccgcctgc
     2281 gggtccctgg gctacgcgat ccacgacgcc cggtacgcgg gtcgggtggt ggcggtgacg
     2341 gacaacctcg tgccctatcc cctggccccc cgtatctcca taccccagta cctggtggat
     2401 cacgtcgcgg tggtggactc catcggggac ccctccaaga tcgccaccgg ggccacccgt
     2461 atatcccggg atcccgtcca gctccggatc gcagagttgg ccttcggact catcaagtcc
     2521 acgggctatc tgaaggacgg ctgttccttc cagaccggag gcggcggccc gagcctggcc
     2581 gcggcccggt acgtccggga ttacatgaag gagaagggga tccggggcag ctactgcatc
     2641 ggagggatca cgggcttcat ggccgagatg ctgaaggaag gacttttcga ggccctcttc
     2701 gacgtccaga gcttcgacgc cgcggtcacc tcctccatgg cggacaatcc ccggcacatg
     2761 gaggcggacg tctcctgcta cgccaacccc ctgaacaagg gctgcctggt caacgacctg
     2821 gacgtggtgg tcctggccgc cctggacacg gacgtggact tcaacgtgga cgtgctcacg
     2881 ggccacgacg gggtcctccg gggcgcttcc gggggacatt ccgacacggc cgcgggggcc
     2941 aagctggcgg tcatcaccgt gccctccatg cggaacgggg tgccttccat ccggaaccgg
     3001 gtccagaccg tcgtcacccc cggggagacc atcgacgcca tcgtcaccga gcgggggatc
     3061 gccatcaatc cccgccggga ggacctggcc gccgcggccc gggccgcggg cctaccgatc
     3121 aaggacatcc gggatctcaa ggcggagatc gagcgggtaa ccggggtccc cgacgaggtg
     3181 gagttcgcgg acgacatcgt ggggatcgtg gaataccggg acgggacgat catcgactcg
     3241 atccgcaggg tcgtctagga cggcgacagg ccgcgccgga aaggaagcga catgcaacag
     3301 ccgtggatca tcggcgccgc agtcctggcc ggggccttcc tgggcgaccg cctcaagctg
     3361 ccctcgggca tcctgaccgg cgggatgatc gcgggtctgg tggccaaggg tttcgtggag
     3421 gggaacgtgc ccagcggccg ggccctctcg gtgatctccc agctcctggt ggcctacgtg
     3481 gtggtctcca attccgacgt ggccaccatc cgcaagcacc ccgagatcct gcccatcgcg
     3541 gtgggctaca tcgtggccct gaccctattc tgcctgggtg ccgcctgggc catcaacaag
     3601 cttttccaca tcgacctgga gaccgcgatc tacgccaccg cccccggggg cctgtccggc
     3661 atggcccttt ccgcgacgga ggccggggcg gagaccccgg tctccatgat gttccacctc
     3721 ctccggctca ccctgatcct ggtcttcacg cccttcctgg cggcgctgtt cgggaagtga
     3781 acggaccgag gaacagggcg aggggccgct ttatggtttc ccatcgacct tcggcaacag
     3841 ggccagggcc ttcctccagt acccgaggat ctcgtcgatt tccgcgtcca cgacaggatt
     3901 cggaaccttc aggtccgggc gggcgtcgta ccactccagg gatctgcgga cgccctcggc
     3961 gaaggtgact tcgggccgga actccgggac gaaactccgg atcttcgagt tgtcgaacag
     4021 cacgctgacc gccttgtccc ccagaaggcc cgctccgcgt tccgggcgaa tacgggcgat
     4081 gtagtccgag ggaatgtgta ccgtctcggc tcgcactccg agcgcctcgg cgaggatcgc
     4141 gtggatagcg tcccaggtca ggcgctcgtc ggaggtgatg tggaatgcct cggacagggc
     4201 cgcgggattc cccagcagtc ctaccaaccc gacggcgaaa tccgatgcgt gggtgagggt
     4261 ccacagggac tggccgtccc cgggaaccac gaccgccttg cccccccgca tccgccaggg
     4321 gatcgtgtaa tccgacgatc cgaacacccc cgggatccag ccgtcgtcgt aggtgtggga
     4381 cggccgcacg atagtgaagg ggaaggatcg tgcggttccc tcctcccgga gcaccgcctc
     4441 gcaggcgatc ttgagccggg agtattccca gaagggattt tccagggggg tatcctcggt
     4501 gatcacgggg ctcagggacg gcttgcggta ggcggagcag gtgcttatga gcacgtactg
     4561 gcccgcgatc ccctcgaaca gttccagatc cgcccggacg tgctcggggc ggtaggccag
     4621 gaactgcacc acgccgtcga agcgcaggcc cgctagggct gtcttcaccg tttcgggatt
     4681 ccggatgtcc gccgtcaggg tgacgacacc cggcgccgtc ctgtcgggac ggttgccgcg
     4741 gttcaggttg aacacctcga acccctcgga gagcgccctg cgggtacagg ccgcggaaat
     4801 gttgcccgtc ccccccacga atagtatccg catttcttcc ctccccggcg atgcgaggtt
     4861 tttcttgaca cctgacgtct tacaacgtaa gattacggat gaccgtcggg attgtcaata
     4921 gaacctgaac gcagaggatt ccagatgttc gaattcagcc aagacgaagt cgcggaaaag
     4981 aaacgcctca tccgggggct tttggaagag acgggcctca cagggatcta cctcaagaag
     5041 accgcgaatt tctcctgggt caccgccggg gggtacaaca tcgtcggcct ttccaccgag
     5101 ttgggagtgg ccggagtcct gttgactccg gaccggggct tcgtggtctg cagcaacata
     5161 gaagtccccc gtatggagca ggaggagcgg ctggtcgagc agggctacga aatcgtcggc
     5221 ttcccctggt acgaggaccg ggaggctgcc ctggtggctt ccctaggggg gccccggctc
     5281 ggggccgacc acgccttccc gggcgccgtc gacgtgtccg gaaaaatcaa tcccctccgg
     5341 tactccctga cctcctggga gatcgcccgc taccggggac aggcccggga aaccgcccgg
     5401 gccgcggagg agaccgcact ggagatccgg cccggggacg aggagcgggc cgtggtgggc
     5461 cggctttcgg atcggctctg gtcccggggc atggacttca tcgtggcctt ctgcgcggcg
     5521 gacgatcgga tctcccggta ccggcatccc gtggctacgg acgcgaagat ccggaagcgg
     5581 gccatgatca gcgtgaactc ccggcgccgg gggctcatcg tctccatcac ccgtttcgta
     5641 cagttcggga aggtccccgg ggaccttcag cgccggtacg aggacaacgt ctatgtggac
     5701 agcgtcatga tggcggcaac gaagccggga aggcccgcat cggaggtctt ccaggcggcc
     5761 gtacgggcct acgccgagaa gggctatccc gacgagtaca agctccacca tcagggcggc
     5821 gccatcggtt acataggccg ggattacaag gccacggagc gcacgaccga gatcatccgg
     5881 gaaaaccagg ccttcacgtg gaatccctcg atcaccggag ccaagagcga ggataccatg
     5941 ctggcctcgt cctcgggccc gatcgtgctt tcggagcccg tgatctttcc gaccatcctg
     6001 tccgaggcgg gcggattcgt tttccgacgc cccggaatcg cggaactgtg aaacgggaaa
     6061 cgaagtttcc ttaattttag gaggaaccag atgaacgtac agcgagtgtt cgtcgcgacg
     6121 gtgctctgcg tcctggcggc cgtcgccctc acggcggacg gcacgaagga agccaagttc
     6181 ccggagcggg aaatcgaggt catggtcccc tgggccgccg gcggcgcgac ggacgtgacc
     6241 ttccgcgctt tcacgggggt catccagaag tacctggggg tcccgatgat catcagcaac
     6301 aagcccggtg gagcggcggt tcccggctac gtggaggcta tgaccaagag ccccgacgga
     6361 cactacctgg tcgcctgggc gacgccttcc atcacggtga cccacatgca gaacaccccc
     6421 tacgacgcga ccaccttcga accggtcctg aacatcgtga ccgcgccggt atggttcctg
     6481 gtccccgcga actcccccta taaaaacctg gcggacctgg tggcggacgc caagaaacgg
     6541 ccgggcatga tcaacctggg caatgccggc accggcggcg gaacccacat gatcgccctg
     6601 gccttcgaga ccgcggcggg cgccaagttc aaccacgttc cccacgcggg aggcggcccc
     6661 acggtgaccg cggcggtcgc gggccaggtg gacgtgatca ccgtcggtcc ccccgaaggg
     6721 gttccgcagc tcgcgagcgg acagctgcgc tgcctgggcg tcttctcgga caaacggctc
     6781 gagcagttcc cggactaccc gaccgcgaag gagctcggca tccccttcag catgggccag
     6841 tggcggggtt tggcggttcc gaagggaacg gatcccaaga agatcaagat tctccacgac
     6901 gcctttaagg ccgccatggc ggatccggag ttcctggcga tcgcggagaa atccggtctc
     6961 ctcctggact accgggacac cgtgtccttc cggaagctca tccaggagca ggacctggtc
     7021 tacaagacca ccgtccagac caacaagctc ggggacaagt acaagtaatt tgccgccgtg
     7081 ggggacccgt acgccgatgg tacgggaccc ccgcgccgga accggccgtt cgaaaagccc
     7141 cgggcttttt ggacgatccg ccgcatttcc gggctccgcg catccctgtt aaggaacgca
     7201 ccgatgggca gaatcgatat cgtgtacggc ttcgtattgg gagctctctc cctgttgttt
     7261 ttcgtggaga ccctctcc
//
LOCUS       DAOTEI010000176         7253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7325_length_7253_cov_4.926091, whole genome shotgun sequence.
ACCESSION   DAOTEI010000176 DAOTEI010000000
VERSION     DAOTEI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7325_length_7253_cov_4.926091"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..741
                     /locus_tag="P5313_14005"
     CDS             115..741
                     /locus_tag="P5313_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81529.1"
                     /translation="MFAFPVFALPLAYLSVAAWFFPRHREEALARSFGRGLALGLPCA
                     LVHMLVLGWIPEVHGSFLFVFRIWWERFLLPFSLTIAASRLIQGFEDTVRSDAALRRF
                     TAFQFGCFTVLALVSAVRYADSPDTFVLFFNPLLSIAGIFAAVYFLERAVTEAGVYVA
                     LWISAGVLASFAFACAAYLFEARLEWLGAILSLALLGISSYQVRFLMK"
     gene            complement(744..817)
                     /locus_tag="P5313_14010"
     tRNA            complement(744..817)
                     /locus_tag="P5313_14010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(781..783),aa:Arg,seq:tct)
     gene            complement(956..1028)
                     /locus_tag="P5313_14015"
     tRNA            complement(956..1028)
                     /locus_tag="P5313_14015"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(993..995),aa:Pro,seq:tgg)
     gene            1190..3286
                     /gene="fusA"
                     /locus_tag="P5313_14020"
     CDS             1190..3286
                     /gene="fusA"
                     /locus_tag="P5313_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY81530.1"
                     /translation="MAFTTDLIRNVALAGHGGTGKTSLLEHLLFSGGAISKPETVDSG
                     KTVSDYMEDEIARKISLHAAFCHLEWKGKNITFVDTPGSSDFVGDVILAFRSCETALL
                     LVDGKSGVQIETIKLWRELDKREKPRGVFISKLDEERADFDAALADIKEKFKMDPVPV
                     SIPMGTGPSFKGVIDVVNGKAYPLPASGDQKETSADIPADFASAYQEARARLSEAAAE
                     GDDELMEKYLEAGELSQEEILKGLGEAFASNRILPAFAGAGLRNSGCAALLEFLAGIA
                     PSPLALKPEIVKNAKGEEVEFGVDPAKPTTALVIRTQIDQFSGRLSYFKVFSGKLLPD
                     SELYNVRENKKEKVGKLYRAQGKKLEEVPELCAGDIGLTSKVAGLKTNDTLTLHDDQF
                     AFAPLRLPQPVHALAISAVNKKEEDKMSELLHKSAEEDLTFQVRFDGETKETVISGMG
                     ELQINMILEKIKTGAKIAVETRTPRVAYRETITKKAHAEYTHKKQTGGHGQYARVVFD
                     IEPLERGKNYEFENKVFGGAVSKGFIPGIEKGIHQAMEAGVTAGYPVVDVKTMIVDGK
                     EHPVDSSEMAFKIASRGAFRECMRQAGPVLLEPIMNLTVFVEDKYLGDIMSDLSGRRG
                     RISGQQPVGGGIVEVKAQAPQAELLRYSIDLRAMTSGTGSFEVEFDHYSPISGKIAED
                     VTKAAQAFRVQEEEEE"
     gene            complement(3353..>7253)
                     /locus_tag="P5313_14025"
     CDS             complement(3353..>7253)
                     /locus_tag="P5313_14025"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRY81531.1"
                     /translation="PRGRSVLSEVRLVILDEIHAVLDSKRGAFLACQIGALSLLAGEF
                     RRVALSATVRPPETAAAFVGGLRKVTAAGGRPGFRYEPRPVSVVAPPAEKRIDLAVEY
                     PEPPPPDPEAGPGLPESSRYGVLVDTLAGRIRKNRSTLVFTDSRRRAERIAFLLNEKA
                     GAGSAYAHHGSLSKEVRRLVEERLKRGELPCVVATGSLELGIDVGSVDEVVLAGSPPA
                     VSPALQRVGRSGHGVGRTSRGLLFPFHGMDLLRAAALAGAVRDREIEPLSPVENPLDI
                     LAQTVLALCAAEDRGVDALYEDIRTFPPFRDLSREAYDRVVEMLAGRYESARLRELKP
                     RLRYDRISGTLGAAEGTVRLLYSSGGAIPDRGVYSMRLQDGTRIGELDEEFVWERKVG
                     DAFSFGTRSWRIREIGPEAVEVVPLDRPADFVPFWKGEARYRSPVLVRRTLELSASFG
                     SEGFPPGTLDGLGLTEEARAALARFLESQARAQQGVPLAGPACLPLELCSDPGLRGDA
                     VTAVLHTLRGGAVNEPLSLALAAELEETLGVRIETVADEDFVLAVLPLVPGVVPDGAI
                     REALGRLGDPDLRERRLRSRLEGSGVFGAAFREAAGRSLVLPRAGFGKRTPLWITRLR
                     AKRLFDAVSSFGDFPVAAEAWRTCLVDLFDLPEFGRLLDGISGGGIRVPAFRTRAPSP
                     FAREAVWQETNRFLYEGDGSERRSGVSLSDRAIEEALGSPALRPALPAALVSDFISRL
                     RREKPGWAPEDGPALAEWVKERVFLPADEWEALAANLGEAAREALEADPTLGARIARI
                     RPAGGFLDLVVHEERRPEVEADPRALLGEWLRYEGPVDPGRAAAVLGIPEEEARAALA
                     ALAEEGSLAAGVRVSDTSGELFCDESNLELLLRLARKAARPSVDERPASDLPLFLARL
                     QGLSGDRPEHPWEPLAGWAAPIRLWETELLPARRPDYAPALLDRDFEEGRCLWFGAGR
                     ETVGLCAPEDLDLALPDRPAESALVPEVLADFWALRDASGLAPEDCVRSLWEEAWRGA
                     ASSDSWETLRSGIRSDFGRAAAALTRSESRLPESSSFGVPRRIPRALRDRWRGGPPVA
                     GRWFSLGAEGDPDPLDRDQLDRDRVRLLARRWGILCRPLLEREVPALGWARLLRALRT
                     LELAGEILAGRFFAGLGGLQFMSPEAFELFRTLEDGGGIYWMNACDPASLAGVEVEGL
                     SYELPARIASTRLCYRGSRLAAVSLRNGRELRILDDGGDPAGVLAFLRIPRTRRVDPE
                     RKVVVETANGAAAAAGPYAAVLKDLGFEADRGRMVLW"
BASE COUNT         1217 a         2566 c         2425 g         1045 t
ORIGIN      
        1 atcggggaat gggcccggga ccacctggaa ctcctctccc tttattagtg tcgaatcgat
       61 aaccccggtt ctgcttgcgc cggtcggcat tcccgtgcta ccctgcggga cgccatgttc
      121 gcatttcccg tcttcgcact gcccctggcc tatctctccg tcgccgcatg gttcttcccc
      181 cgccaccggg aggaggccct ggcccgaagc ttcggaaggg gcctggccct ggggctcccc
      241 tgcgcgctgg ttcatatgct ggtcctaggc tggattccgg aggtccacgg cagtttcctg
      301 ttcgtgttcc ggatctggtg ggagcgcttc ctcctgccct tttccctgac catcgccgct
      361 tcccgcctga tccagggctt cgaggacacg gtgaggtccg atgccgcgct gcggaggttc
      421 acggccttcc agttcggctg tttcacggtc ctggccctgg tttcggcggt gcggtacgcg
      481 gacagtccgg acaccttcgt actcttcttc aatcccctgc tttccatagc cgggatcttc
      541 gcggccgtgt acttcctgga gcgggcggtg acggaggcgg gtgtctacgt ggccctctgg
      601 atctccgcgg gggtcctggc cagcttcgcc ttcgcctgcg ccgcctacct cttcgaggcg
      661 cgcctcgagt ggctcggggc catcctgtcc ctggccctgc tggggatttc ctcgtaccag
      721 gtccgattcc tgatgaaatg aagcgggacc agagggactc gaacccccga cctaccgctt
      781 agaaggcggt tgctctatcc ggttgagcta tgatcccctg cgggggtggc caataagtcg
      841 gctacttatt ggccacccct tgcagtttcc aacgttctat tgagaacgtt agaagatgct
      901 accttaatgc cagctgtcca aaaagctaac cgagcttttt gaacagcctg gaatctcggg
      961 acgggcggat ttgaaccgcc gatatccagc tcccaaagct ggcgccttag ccgctaggca
     1021 acgtcccgga atacccgcgt actctaggcc gaaggcaaat cctttgtcaa tcgaagcgcc
     1081 ccgcccgggg tccccggctt tttcccttga caaccggaaa aatcgggttc tatcatggcg
     1141 gcagtccttt aggctcggga tccccggcct taacaatcaa ggagcgctta tggcctttac
     1201 gaccgatctc atccggaacg tggccctggc gggacacgga gggacgggaa aaacttcctt
     1261 gctcgaacac ctgctgttct ccggcggagc catctccaag ccggaaaccg tggattcggg
     1321 caagactgtc agcgattaca tggaggacga gatcgcccgc aagatctccc tccacgccgc
     1381 cttctgccac ctggagtgga agggcaagaa tatcactttc gtggacaccc ccggatcctc
     1441 ggatttcgtg ggcgacgtga tcctggcctt ccgttcctgc gagacggccc tgctcctggt
     1501 ggacggcaag agcggagtcc agatcgagac catcaagctg tggcgggagc tggacaagcg
     1561 ggaaaagccc cgcggcgtct tcatctccaa gctggacgag gaacgcgccg acttcgacgc
     1621 ggccctggcg gacatcaagg agaagttcaa gatggacccc gttcccgtct ccattcccat
     1681 ggggaccggg ccgtccttca agggcgtcat cgacgtggtg aacggcaagg cctaccccct
     1741 gcccgcctcc ggggaccaga aggaaacctc cgccgacatc cccgcggact tcgcctcggc
     1801 gtaccaggaa gcccgcgccc ggctttccga ggccgccgcg gagggggacg acgagctcat
     1861 ggagaagtac ctggaggccg gggagctctc ccaggaggag atcctcaagg gtctcgggga
     1921 ggccttcgcg tccaaccgca tcctgcccgc cttcgcggga gccgggctcc ggaactccgg
     1981 ctgcgcggcc ctcctcgagt tcctggccgg gatcgccccc tcccccctgg ccctcaagcc
     2041 cgagatcgtg aagaacgcca agggagagga agtcgagttc ggggtcgatc cggccaaacc
     2101 gaccaccgcc ttggtcatcc gcacccagat cgaccagttc tccgggcgtc tgtcctactt
     2161 caaggtcttc tccggcaagc tccttccgga ctcggaactc tacaacgtcc gggagaacaa
     2221 gaaggagaag gtcggcaagc tctaccgcgc ccagggcaag aagctcgagg aagtcccgga
     2281 actctgcgcg ggggacatcg ggctgacttc caaggtggcg ggcctcaaga ccaacgacac
     2341 cctgaccctg cacgacgacc agttcgcctt cgcccccctg cgcctgcccc agccggtcca
     2401 cgccctggcc atctccgcgg tgaacaagaa ggaggaggac aagatgtccg agctcctgca
     2461 caagtccgcg gaggaggacc tgaccttcca ggtccggttc gacggggaga ccaaggagac
     2521 cgtcatctcc ggcatgggcg agctccagat caacatgatc ctggagaaga tcaagaccgg
     2581 ggccaagatc gccgtggaga cccggacccc gcgggtggcc taccgggaga ccatcaccaa
     2641 gaaggcccac gcggagtaca cccacaagaa gcagaccggg gggcacggac agtacgcccg
     2701 ggtcgtcttc gacatcgagc ccctggagag gggcaagaac tacgagttcg agaacaaggt
     2761 cttcggcgga gccgtgtcca agggattcat tccgggaatc gagaagggca tccaccaggc
     2821 catggaggcc ggcgtaacgg cgggctaccc cgtggtggac gtcaagacca tgatcgtgga
     2881 cggcaaggag catcccgtgg actcctcgga gatggccttc aagatcgcca gccgcggcgc
     2941 cttccgggag tgcatgcgcc aggcgggtcc cgtcctcctg gaacccatca tgaacctgac
     3001 cgtcttcgtg gaggacaagt acctggggga catcatgtcc gacctctccg gccgccgcgg
     3061 ccggatctcc ggccagcagc ccgtgggcgg cgggatcgtg gaggtcaagg cccaggctcc
     3121 ccaggcggag ctcctgcggt actccatcga cctcagggcc atgactagcg gcaccggttc
     3181 cttcgaggtg gagttcgacc actactcccc gatctccggg aagatcgccg aggacgtcac
     3241 caaggcggcc caggccttcc gggtgcagga ggaagaagaa gaataaagaa acggggcgcg
     3301 gtgcgccccg tttctttatt gatcggtttc acggcgatgc cgtgaaaccg gttcaccaaa
     3361 ggaccatccg cccccggtcc gcctcgaagc ccaggtcctt gaggaccgcg gcatagggtc
     3421 cggccgcagc cgccgcgccg ttcgcggttt ccaccaccac cttccgttca ggatccaccc
     3481 gtcgggtccg gggaatcctc agaaaggcca agaccccggc cggatcccct ccgtcgtcga
     3541 ggatccgcag ctcccggccg ttccgcaggg agaccgcggc caggcggctt ccccggtagc
     3601 agagtctggt ggaagctatc cgggccggga gttcatagga cagcccctcg acctccaccc
     3661 cggccaagct ggcggggtcg caggcgttca tccagtaaat tcccccgccg tcctccaggg
     3721 tccggaagag ctcgaaggcc tcgggactca tgaactgcag gcctccgagt cccgcgaaga
     3781 atctccccgc caggatctcc cccgccagct ccagggtacg cagggcccgg agcagacgcg
     3841 cccagcccag ggccgggact tccctctcca ggaggggccg acagaggatc ccccaccgtc
     3901 gggccaggag ccgcacccgg tcccggtcca gctggtcccg gtccagggga tcgggatccc
     3961 cctccgcccc cagggaaaac caccggcccg ccacgggggg cccgccccgc caccggtccc
     4021 gcagggcccg gggtattcgg cgggggacgc cgaaggaaga ggattccgga agtctcgact
     4081 cggatcgagt gagggcggcc gcggcgcggc cgaaatccga ccggatcccg gaccggaggg
     4141 tctcccagga atccgaggac gcggcacccc tccaagcctc ttcccagagg gagcggacgc
     4201 agtcctcggg ggccaggcca gaggcgtccc ggagggccca gaagtccgcg aggacctccg
     4261 gtaccagggc ggattccgcg ggtcgatccg ggagggcgag gtccaggtcc tcgggtgcgc
     4321 agagcccgac ggtctcccgg cccgccccga accagaggca ccggccttcc tcgaagtccc
     4381 ggtccaggag ggcgggggcg tagtccggcc gtcgggcggg cagaagctcc gtctcccaga
     4441 ggcggatcgg ggcggcccag cccgccaggg gctcccaggg atgctccgga cggtcccccg
     4501 agagtccctg gagccgcgcc aggaagaggg ggaggtccga ggcgggccgt tcatccaccg
     4561 agggccgggc cgccttccga gccaggcgga ggaggagctc caggttggac tcgtcgcaga
     4621 acagctcgcc ggaggtgtcc gagacccgca cccccgcggc cagggagccc tcctcggcca
     4681 gggcggccag tgcggcacgg gcctcctcct ccgggattcc caggacagcg gcggcccgcc
     4741 ccggatccac gggaccctcg taccgcagcc actcccccag gagggcccgg ggatccgcct
     4801 cgacctcggg tctgcgctcc tcgtggacga ccaggtccaa gaatcccccg gccgggcgga
     4861 tccgggcgat ccgggcgccc agggtcgggt ccgcttccag ggcctccctc gccgcctcgc
     4921 ccaggttcgc cgcgagggct tcccactcgt ccgcgggcag gaagacccgc tccttgaccc
     4981 attccgccag ggccgggccg tcctcggggg cccagcccgg cttctcccga cgaagccggg
     5041 agatgaaatc ggacaccagg gcggcgggca gggccgggcg gagggccggg gagcccaggg
     5101 cctcctcgat cgcccgatcg gacagggaaa ccccggaccg gcgctcggac ccgtccccct
     5161 cgtacaggaa ccggttcgtc tcctgccaga ccgcttcccg ggcgaagggg ctgggcgccc
     5221 gggtgcggaa ggccgggacc cggatgcctc cgcccgagat cccgtccagg agccggccga
     5281 actccggtag gtcgaagagg tcgaccaggc aggtcctcca ggcctccgcg gccaccggga
     5341 agtccccgaa ggagctgacc gcgtcgaaca ggcgcttggc ccggagccgg gtgatccaca
     5401 ggggggtacg tttcccgaag cccgcccggg gcaggacgag ggaccgcccc gcggcctccc
     5461 ggaaggcggc cccgaagacc ccggatccct ccaggcggga ccggagacga cgctccctca
     5521 agtccgggtc ccccaaccgt ccgagggcct cccggatcgc cccatccggg accacgcccg
     5581 gcacgagggg aaggacggcc aggacgaagt cctcgtccgc caccgtctcg atcctcaccc
     5641 ccagggtctc ctccaattcg gccgccaggg ccagggagag aggctcgttc accgccccgc
     5701 cccggagggt gtggaggacc gcggtgaccg cgtcgccccg gagcccgggg tcggagcaga
     5761 gctccagggg gagacaggca ggtccggcga gagggacacc ctgctgggcc cgggcctggg
     5821 attccaggaa gcgggccagg gcggcccggg cctcctccgt cagtcccagg ccgtccaggg
     5881 tgcccggagg gaaaccttcg gagccgaagg acgcggagag ctccagggtc ctgcggacca
     5941 ggaccgggct ccggtaccgg gcctccccct tccagaaggg cacgaaatcc gcgggccggt
     6001 ccaggggcac gacctccacg gcctcggggc cgatctcccg gatccgccag gaccgggtcc
     6061 cgaaggagaa ggcgtccccg accttccgct cccagacgaa ctcctcgtcc agttccccga
     6121 tgcgggtacc gtcctgaagg cgcatcgagt acaccccccg gtccggaatg gcgcctccgg
     6181 aggagtagag gagccggacc gttccctccg cggcgcccag ggtgccggag atacggtcgt
     6241 agcggaggcg gggcttgagc tcccggagcc gggcggactc gtatcgcccg gccagcatct
     6301 ccacgacccg gtcgtacgcc tcccgggaga ggtcgcggaa gggcgggaag gtccggatgt
     6361 cctcgtagag ggcgtccacg ccccggtcct cggccgcgca gagggccagg acggtctggg
     6421 ccaggatgtc caggggattc tccaccgggg acagcggctc gatctcccgg tcccggaccg
     6481 ccccggccag ggccgcggcg cgcaggaggt ccatcccgtg gaagggaaat aggaggcccc
     6541 ggctcgtccg gccgaccccg tggccggacc gccccacccg ctggagggcc ggggacaccg
     6601 cgggagggga gcccgccagg accacctcgt ccacggaccc cacgtcgatg cccagctcca
     6661 gggaccccgt ggccaccacg caaggcaact ccccgcgctt gaggcgctcc tcgaccaggc
     6721 ggcggacctc cttggagagg gagccgtggt gggcgtaggc ggagcccgcc ccggccttct
     6781 cgttcaggag gaaggcgatc cgttcggccc ggcgacggga atccgtgaac accagagtcg
     6841 agcggttctt ccggatccgc ccggccaggg tatccaccag gaccccgtac cgggaggatt
     6901 ccgggagccc gggtccggcc tccggatccg ggggcggcgg ctcggggtac tccacggcca
     6961 ggtcgatcct cttctccgcg gggggagcga cgacggacac cggccggggc tcgtaccgga
     7021 aacccggccg ccccccggcg gcggtaacct tccgcaggcc tccgacgaag gcggctgccg
     7081 tctccggcgg ccgtaccgtg gcggagaggg ccacccgccg gaactccccg gccaggaggg
     7141 acagagctcc gatctggcag gccaggaagg ccccgcgctt ggagtccagg accgcgtgga
     7201 tctcgtccag gatgaccagg cggacctccg agaggacgga cctcccccgg gga
//
LOCUS       DAOTEI010000177         7253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7326_length_7253_cov_4.415949, whole genome shotgun sequence.
ACCESSION   DAOTEI010000177 DAOTEI010000000
VERSION     DAOTEI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7326_length_7253_cov_4.415949"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..399
                     /locus_tag="P5313_14030"
     tRNA            313..399
                     /locus_tag="P5313_14030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:347..349,aa:Ser,seq:gga)
     gene            584..682
                     /gene="ffs"
                     /locus_tag="P5313_14035"
     ncRNA           584..682
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5313_14035"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            895..>2079
                     /gene="dnaX"
                     /locus_tag="P5313_14040"
     CDS             895..>2079
                     /gene="dnaX"
                     /locus_tag="P5313_14040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRY81532.1"
                     /translation="MAYEITAARKRPRGFEELAGQDFVSTTLKASLDTGRIAHAYLFS
                     GPRGCGKTSTARILAKSLNCEGGPTSKPCGKCASCQSIQRGAALDVIEIDGASNTSVD
                     NVRQIKDEVLFPPSSGRYKVYIIDEVHMLSNSAFNALLKTIEEPPPYIVFIFATTELH
                     KVPATIKSRCQQFNFRLIPLDVIRERLREAATELAVDADEEALLWIAKEATGSLRDAY
                     TLFDQAVSFSEGRITYEKVRDKLGLVGVDRMNDLFERCVRGERSEALDWVEDILSRGV
                     SPEQCVADAAEYLRSLLLIKSGVSRESVLGDSPSRYSPEVLEALSSQRIERALAEILD
                     LHRDLRYTVDPRLELELAAARLCSIRDWISPSEAARAVEELRAWTLSADAASAPALPG
                     QKK"
     assembly_gap    2080..2089
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2090..2699
                     /locus_tag="P5313_14045"
     CDS             <2090..2699
                     /locus_tag="P5313_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81533.1"
                     /translation="PDPREPSEGPRAVRPARGTAAQGRLRDSGEADPGELSGSSDPGD
                     AEAAGSGAGPSAGRTAPSSEPGSDARAVREAVLARLAKSHVVLASALDKSLSWTVTEN
                     RLEILFRSGYEEAMARRDLAVLVKAAGEAAGRVLKVELRVEEAPKEAPRTSRTAAEDD
                     LPPPDEEEPETDAVEIVERVFRGKRVEGSAPPPRSGEADGYF"
     gene            2686..3024
                     /locus_tag="P5313_14050"
     CDS             2686..3024
                     /locus_tag="P5313_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRY81534.1"
                     /translation="MDISEILKMLGDPRALQERMREAQDRMARTTATGSAGGGMVRIT
                     LNGSFEMTGIEIAPEVLDPPDPVLLGDLVRAAHNDAAARLRETLQRDLTGAAGGLPIP
                     PDLFGGGAPR"
     gene            3021..3608
                     /gene="recR"
                     /locus_tag="P5313_14055"
     CDS             3021..3608
                     /gene="recR"
                     /locus_tag="P5313_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRY81535.1"
                     /translation="MKVLEDLIGLLSRLPGIGRKSASRIAFHLLKADESYAQALADRI
                     LTLRGSVRFCSVCGAYAETDPCPICSSPGRDRSLLCVVEQPQDVLTVEASREYRGLYH
                     VLGGLLSPLDGVGPEALRIDRLSERVRGGEVREVVLATNPTVEGDTTALYIRKVLENA
                     GVNVTRLATGIPVGGDLEYADKLTLARSFRGRVKL"
     gene            complement(3657..5057)
                     /locus_tag="P5313_14060"
     CDS             complement(3657..5057)
                     /locus_tag="P5313_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81536.1"
                     /translation="MERADRAARDAVLRKLFPGLGAGQDPEIGRYFRLRSEGRTAEAL
                     ALFNQVLKIRYPDEARRAALFNLFRRSDPRFPAFHDDLLWDLYDSSSVRLRETLDRVC
                     RPVSGIAPKDAYRLLCALEDVVRLLPEGRRAALARLDELCALAELLGHRTREIQRVRA
                     LAASYFDQGAGDFDLSRAELSTADVERVEIPAGVERREDRVLAYCFKYWKSAEDPAFE
                     RLVLLYSRKRGTPHHEILRAIRDGRAAGAPDEEILGRVSALLSPGYSYTTQGDLYMRR
                     AWKALKERLEPGPAVPRSGNAESPARDRPVRGEGEPETPGKAAPGRRTSRRTDARRVA
                     EGTRKPPRKRTARGDPARSAESPRPAAPGGSVSDRIKALSGKRYDVYREEFLARVGPA
                     IRDRLSERRIKPKDAFEDPLGRAEDLVRDFLEKNYANPYMDWEGSDTRARVEGLGFSV
                     PDLDGIIALWYSRRGK"
     gene            complement(5156..6016)
                     /locus_tag="P5313_14065"
     CDS             complement(5156..6016)
                     /locus_tag="P5313_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRY81537.1"
                     /translation="MIINHNLSAMFADRSLRVTNGNVDKGMEKLASGLRINRAGDDAS
                     GLAVSEKMRSQIRGLQRASKNAADGISFIQTAEGFLQESQDILQRMRELAVQASNGIY
                     SAEDRMQIQVEVSQLVDEIDRIASHAQFNGMNLLTGRFARETGENIVTGSMWFHIGAN
                     MDQRERAYIGTMTAKGLGVRNVGDESILSIATPDDSNRAIGTLDAALKVVNKQRADLG
                     AYQNRMEHAIRGIDIGAENLQAAESRIRDADMANEMVDYTKNQILAQAGNAMLAQANQ
                     KTQQVLQLLQ"
     gene            6406..6765
                     /locus_tag="P5313_14070"
     CDS             6406..6765
                     /locus_tag="P5313_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81538.1"
                     /translation="MTVRRIAELLEARVHTGEDDLDLDVSAACGADLMSDVMAFVKEK
                     VVLLTGLVNPQAIRTADLLDIRVVVFVRGKTPSRDMVEAARESGMILLSTNYSMFISC
                     GRLYEAGLRAGGTREIR"
     gene            6776..>7253
                     /locus_tag="P5313_14075"
     CDS             6776..>7253
                     /locus_tag="P5313_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY81539.1"
                     /translation="MTLTFTVPGGDFNQAGRASGEVKKVLARLGVDPGIIKRAAVAMY
                     EAEINMVIHAGGGTAVAEISPERVVLTLEDTGPGIPDLDLAMREGYSTAPEWIRELGF
                     GAGMGLPNMRRNSDELSIDSAPGRGTRVTIVLRLDGEGASVENRAAPAAAPAGTSPA"
BASE COUNT         1119 a         2461 c         2469 g         1194 t
ORIGIN      
        1 gcgagagcgg ttgaatcggg cggtctcgaa aaccgttgaa cccgtaaggg ttccgggggt
       61 tcgaatcccc ctctctccgg aaatgtccta ccatccgttc gtcgggggat tcgaaccgag
      121 cccctcgccg accggaggga ggcaaggcga tacggagatc gccgctagag gggcgaggcg
      181 ggttcgagga gcttccagga cgggagcgac gagaaccgaa tccccctctc tccggagaat
      241 atgcgacgta ttcgacggag gtttccaccg cggccttctc cggtgcgtcg atgtaataac
      301 gtggtccgac ttggagagat gaccgagcgg ccgaaggtgc gcgattggaa gtcgcgtgta
      361 cccgtaaggg taccgagggt tcaaatccct ctctctccgt acgaaatgga aagacgagcc
      421 cgcaggcccg tctttcgggg gctgtccaaa aagcccggac ggctttttgg acagttggtt
      481 cccgtccggc agtttctagc gttctcggag agaacgctag aaactgcaag gtttgtgcca
      541 tgcgtaaccg acgcatggca caaaccggtc ctcgggcgtt cgtagccagg ccccacgcta
      601 acggtcgccg cccaaccccg ccaggcccgg aagggagcaa cggtaagcgg tagggcgttg
      661 tgccgtggtc ggcctggcta ccgacgttcg agggcttttt tattgaatcc gacaggatgt
      721 acaggatcac ctaggaagta ccggatggtg cttggagatg gtccgtccgt ttttccatcc
      781 tgaatcccgg gaatccttcc gaattctgtt catcctgtct cgtctttctc cggcatagac
      841 cgcctgtacg ccaccctttg ccgaaagcgc cggccccgtg gtatccttcc ccccatggca
      901 tacgagatca ccgcggcccg gaagcggccc cggggcttcg aggaactggc ggggcaggac
      961 ttcgtctcca ccaccctcaa ggcctccctg gacaccggcc ggatagccca cgcctacctc
     1021 ttttcgggcc cccggggctg cggaaagacc agcacggccc gcatcctggc caagagcctc
     1081 aactgcgagg gcggcccgac ctccaagccc tgcgggaagt gcgcctcctg ccagtccatc
     1141 cagcgggggg cggccctgga cgtgatcgag atcgacgggg cctcgaacac ctccgtggac
     1201 aacgtccggc agatcaagga cgaggtcctc ttccccccct cctcggggcg ctacaaggtc
     1261 tacatcatcg acgaagtcca catgctctcg aactcggcct tcaacgccct cctgaagacc
     1321 atcgaggagc cgccccccta catcgtcttc atcttcgcca ccacggagct ccacaaggtc
     1381 ccggccacca tcaagagccg gtgccagcag ttcaacttcc gcctcatccc cctggacgtc
     1441 atccgggaac gactccgcga ggcggccacg gaactggccg tggacgccga cgaagaggcc
     1501 ctgctctgga tcgccaagga ggccacgggc tccctccggg acgcctacac cctcttcgac
     1561 caggccgtct ccttttccga gggccggatc acctacgaga aggtccggga caagctgggc
     1621 ctcgtggggg tcgaccggat gaacgacctc ttcgagcgct gcgtccgggg cgagcgctcc
     1681 gaggccctgg actgggtgga ggacatcctg tcccgggggg tgtccccgga gcagtgcgtg
     1741 gcggacgcgg cggagtacct ccggtccctc ctcctcatca agagcggggt ctcccgggaa
     1801 tccgtgctgg gggactcccc gtcccggtat tccccggagg tcctggaggc cctttcctcc
     1861 cagcgcatcg agcgggccct ggcggagatc ctggaccttc accgggacct gcgctacacc
     1921 gtggatcccc gcctggagtt ggagctggcg gccgcgcggc tctgctccat ccgggactgg
     1981 atctccccct ccgaggccgc acgggccgtg gaggaactcc gggcctggac cctctccgcg
     2041 gacgccgcct cggcgcccgc ccttccgggg caaaaaaaan nnnnnnnnnc ccggatcccc
     2101 gggagccgtc cgaggggccc cgcgccgtcc gtcctgcccg gggtaccgcg gctcaggggc
     2161 ggcttcggga ttccggggag gccgacccgg gcgagttgag cggatcctcg gatcccgggg
     2221 acgccgaggc cgcggggtcg ggcgccggcc cgtcggccgg ccgcaccgct ccctcctccg
     2281 agccgggctc cgacgcccgg gccgtccggg aggccgtcct ggcccggctg gccaagtccc
     2341 acgtggtcct ggccagcgcc ctggacaaat ccctttcctg gacggtgacg gaaaaccgcc
     2401 tggagatcct cttccggtcc gggtacgagg aggccatggc ccgtcgcgac ctggccgtgc
     2461 tggtcaaggc cgcgggggag gccgcgggcc gagtcctgaa ggtggagctc cgggtggagg
     2521 aggccccgaa ggaggccccc cggacttccc ggaccgcggc ggaggacgac ctgccgcccc
     2581 cggatgagga agagccggag acggacgcgg tggagatcgt ggaacgggta ttccggggca
     2641 agcgcgtgga gggttccgcc ccgccgcccc gttcgggaga agccgatgga tatttctgag
     2701 atactcaaga tgctcgggga tccccgggcc ctgcaggagc gcatgcggga agcccaggac
     2761 cggatggccc gcacgaccgc cacgggttcc gccggggggg gcatggtccg gatcaccctg
     2821 aacggttcct tcgaaatgac cgggatcgag atagccccgg aggtcctgga tcccccggac
     2881 cccgtcctgc tcggggacct cgtgcgggcc gcccacaacg acgccgcggc ccggctccgg
     2941 gagaccctgc agagggacct gacgggcgcg gccggaggcc tgcccatccc gccggacctc
     3001 ttcgggggcg gagcgccccg gtgaaggtcc tggaggacct catcggtctc ttgtcccggc
     3061 tgcccgggat cggccggaag agcgcttccc gcatagcctt ccacctgctc aaggccgacg
     3121 aatcctacgc ccaagccctg gcggaccgga tcctgaccct ccggggttcg gtccggttct
     3181 gctcggtctg cggcgcctac gccgagacgg atccctgccc gatctgttcc tccccgggcc
     3241 gggaccggtc cctcctgtgc gtggtggaac agccccagga cgtcctgacc gtggaggcct
     3301 cccgggaata ccggggcctg taccacgtac tggggggcct gctctcgccc ctggacgggg
     3361 tggggccgga ggccctgcgc atcgaccgcc tttccgagcg cgtccggggc ggggaggtcc
     3421 gggaggtggt cctggccacc aacccgaccg tggaggggga cacgacggcc ctgtacatcc
     3481 ggaaggtcct ggaaaacgcc ggggtgaacg taacccggtt ggccacgggg atccccgtgg
     3541 ggggtgacct ggagtacgcg gacaagctga ccctggcccg gtccttccgt gggcgcgtga
     3601 agctctagtt cctcgggatc cgggccgcct acgccccggg gggaaatcga tccggactac
     3661 ttcccccgcc gggagtacca aagggcgatg atcccgtcca ggtccgggac ggagaacccc
     3721 aggccctcca cccgggcccg ggtgtccgat ccctcccagt ccatgtaagg attcgcgtag
     3781 ttcttctcca ggaagtcccg gaccaggtct tccgcccggc ccaggggatc ctcgaaggcg
     3841 tccttgggct tgatcctccg ctcggacagc cgatcccgga tggccggccc gacccgggcc
     3901 aggaattcct cccggtagac gtcgtagcgc ttgccggaaa gggccttgat gcggtccgag
     3961 acggagcccc ccggcgcggc gggccggggg gattcggccg atcgggccgg gtctccccgg
     4021 gcggtacgtt tccggggagg cttccgcgtc ccctccgcaa cccgacgggc gtccgtcctc
     4081 cgggaggtcc gcctcccggg ggcggccttc ccgggcgtct caggctcgcc ctctccgcgg
     4141 accggccggt cgcgggccgg agactcggcg ttcccgctcc tcgggacggc ggggccgggc
     4201 tccagccgct ccttcagggc cttccaggcc ctccgcatgt acaggtcccc ctgggtcgtg
     4261 taggagtagc ccggggagag gagcgcggac acccggccca ggatctcctc gtccggcgcg
     4321 cccgcggcgc ggccgtcccg gatcgcccgg aggatctcgt gatggggggt cccgcgcttc
     4381 ctcgagtaca ggaggaccag ccgctcgaag gccgggtcct ccgcggactt ccagtacttg
     4441 aagcagtagg ccaggacccg gtcctcccgg cgctccacgc ccgcgggaat ctcgacgcgt
     4501 tccacgtcgg cggtgctcag ctccgcccgg gacaggtcga agtcccctgc gccctggtcg
     4561 aaataggagg cagccagggc gcggacccgc tggatctccc gggtccggtg ccccaggagt
     4621 tccgccagtg cgcaaagctc gtccagccgg gccagggccg ccctccggcc ctccggcagg
     4681 agccggacca cgtcctccag ggcacaaaga agcctgtagg cgtccttggg cgctatgccg
     4741 gagacgggtc ggcataccct gtccagggtt tcccggagcc ggacggagga ggaatcgtag
     4801 agatcccaaa gcaggtcgtc gtggaaggcg ggaaagcggg ggtccgagcg gcggaagagg
     4861 ttgaacagtg ccgccctccg ggcctcgtcg ggataccgga tcttcaggac ctggttgaag
     4921 agggccaggg cctcggcggt gcggccttcc gagcgcagcc ggaaataccg gccgatctcg
     4981 gggtcctgtc ccgctccgag acccgggaag agtttccgga gtacagcgtc gcgcgcggcg
     5041 cggtccgccc gttccatgat gggcgattat aggggaggcc gccctcccga tcaagccgag
     5101 aaaaaggccg cccccggggg ggcggccttc ggtttcactt cccggggtcc tggtcctact
     5161 ggaggagctg gaggacctgc tgggttttct gattggcctg ggcgagcatc gcgtttccgg
     5221 cctgggcgag gatctggttc ttcgtgtagt ccaccatctc gttggccatg tcggcgtccc
     5281 ggatgcggct ctcggcggcc tggagattct cggcgccgat gtcgatcccg cggatcgcgt
     5341 gctccatgcg gttctggtac gctcccagat ccgcccgctg cttgttgacg accttgaggg
     5401 ccgcgtccag ggttccgatg gcgcggttgg agtcgtccgg ggtggcgatg gacaggatac
     5461 tctcgtcccc gacgttgcgc acgccgagtc ccttggcggt catcgtgccg atgtaggcgc
     5521 gctcccgctg atccatgttg gcgccgatgt ggaaccacat ggatccggtg acgatgttct
     5581 ccccggtctc gcgggcgaaa cggcccgtga gcaggttcat gccgttgaac tgggcatggc
     5641 tcgcgatgcg gtcgatctcg tcgaccagct gcgaaacctc gacctggatc tgcatgcggt
     5701 cttccgcgct gtagatcccg ttcgacgcct gtacggccag ttcccgcatc cgctggagaa
     5761 tgtcctggct ctcctggagg aatccctccg cggtctggat gaaggagatg ccgtccgcgg
     5821 cgttcttgga cgcccgctgg agcccgcgaa tctgcgagcg catcttctcc gagaccgcga
     5881 gtcccgaagc gtcgtccccg gcgcggttga tccgcagtcc ggacgcgagc ttctccatcc
     5941 ccttgtccac gttcccgttc gtgaccctga gggagcggtc ggcgaacatc gcactgaggt
     6001 tgtgattgat gatcataacc tgtttctcct ttccttaggt gacaacggcc cgtcttcggg
     6061 gccgttcggc ttccttgccg gtatcgtgtc tggttcgacg tcgctcctcc gggacctacg
     6121 gccccgttga agtgataccg tgggccgccg gtcggcaaaa aaccgtccgg cgccctctct
     6181 aggtatcggt aatccgtaaa cgggctttag gaattccgcg aaggcacgcc gccgccggac
     6241 aatcgggggc tcggaaccat aggagggaca agatgaacag gatccagaga ggatgagcag
     6301 gatagaagga aggggatgca tcgatctcgg ccttccatca tgttcatctc gatccatctt
     6361 gtagatcctg ttggatcgat ggtatcttgc cgccggggag acgccatgac cgtcagacgg
     6421 atagcggaac tgctggaggc ccgggtgcat acgggcgagg acgacctgga cctggacgtg
     6481 agcgccgcct gcggcgcgga cctgatgagc gacgtgatgg ccttcgtcaa ggagaaggtc
     6541 gtcctcctca cgggcctcgt gaacccccag gccatccgca ccgcggacct cctggacatc
     6601 cgggtggtgg tcttcgtgcg cggcaagacc ccttctcggg acatggtcga ggcggcccgg
     6661 gaatccggca tgatactcct ttccacgaac tactccatgt tcatctcctg cggacgcctt
     6721 tacgaggccg gcctccgggc gggcgggacc cgggagatcc gctaagcccg gtccgatgac
     6781 cctgaccttc accgtccccg gcggggactt caaccaagcg ggccgcgcct ccggggaggt
     6841 caagaaggtc ctggcccggc tgggcgtgga tcccgggatc atcaagcggg ccgcggtggc
     6901 gatgtacgag gcggaaatca acatggtcat ccacgccggg ggaggcacgg cggtggccga
     6961 gatcagcccg gaacgggtgg tcctgaccct ggaggatacc ggccccggca tcccggacct
     7021 ggacctggcc atgcgggagg gctactccac ggccccggag tggatccggg aactcggctt
     7081 cggggccggg atggggctgc ccaacatgcg gcggaacagc gacgagctat ccatagattc
     7141 cgcgcccgga cggggcaccc gggtgaccat cgtcctgcgg ctggacgggg aaggcgcgtc
     7201 cgtcgagaac cgggccgccc cggccgcggc gcccgccgga acctccccgg cgg
//
LOCUS       DAOTEI010000178         7235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7352_length_7235_cov_4.662953, whole genome shotgun sequence.
ACCESSION   DAOTEI010000178 DAOTEI010000000
VERSION     DAOTEI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7352_length_7235_cov_4.662953"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="P5313_14080"
     CDS             complement(<1..483)
                     /locus_tag="P5313_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81540.1"
                     /translation="MPFNHVSRIDDRVPVRNAIVSVYDKIGLPDLARGLVESCPGIRI
                     YSTGGTLSALRTILGPAAERILVSMEDYTGQPEMKGGLVKTLDWRIYLGLLSEPYDPE
                     HAADRARSGAVAFDLVVGNLYPFGSVSSASGPFEAARQNIDIGGPCMLRAAAKNFLRV
                     A"
     gene            complement(599..1366)
                     /gene="folE2"
                     /locus_tag="P5313_14085"
     CDS             complement(599..1366)
                     /gene="folE2"
                     /locus_tag="P5313_14085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRY81541.1"
                     /translation="MIDIQSMRDERRIPIQKVGVKGLRYPIMLRDKERGTQWTTAVAN
                     LYADLPHDFKGTHMSRFIEVFNAHREDLSMPRFLGMLGEIRQALDAETAYSDIHFPYF
                     IEKAAPVSGAKSVMSYDCVYSGRVSDQVRQFRVSVSVPVQTVCPCSKAISAHGAHNQR
                     GVVTATVEVGPFFWLEDLIAVIESAASCDVYTLLKRDDEKYVTEKAFDNPRFVEDVVR
                     DALRGIDGMGIFKGFSVEAENFESIHNHSAYAYVEYG"
     gene            complement(1363..2241)
                     /gene="folD"
                     /locus_tag="P5313_14090"
     CDS             complement(1363..2241)
                     /gene="folD"
                     /locus_tag="P5313_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRY81542.1"
                     /translation="MAASIIDGKALAADIREDVKRRASALAARGVVPGLAVILVGEDP
                     ASVSYVTSKEKACEEAGMRSFERRLPASTPQPELVALVETFNRDPAVHGILVQLPLPD
                     HIDEDAVIAAISPDKDVDGFTPVNVGRMILGQPCFLPCTPHGILKLVERAGVPTKGAH
                     VVVVGRSNIVGKPLANLFVRKELNSTVTVCHTGTRDLAEHTRRADILIACAGRPRLIT
                     GDMIRPGACVIDVGVNRVEDLSAAKGYRLVGDVDFQSALPVAGSITPVPGGVGPMTIA
                     MLLSNTVESAERTVRA"
     gene            complement(2366..3562)
                     /locus_tag="P5313_14095"
     CDS             complement(2366..3562)
                     /locus_tag="P5313_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY81543.1"
                     /translation="MPFPPFSRRIPAPREPNALGRALAEAQASGRRVINLAESNPTRV
                     GAALPAEKVLRALARTESLSYEPDPRGLPAARSAVASWYASRGTSVDPSDIFLAASTS
                     EAYGWLFKLLADPGDSVLVPKPGYPLFEYLSGLEGVEAVPYPLEYRHPAGWSADLDSL
                     EAALKSRRPKAVILIHPNNPTGSFVGRRERRRILDLCGEAGTAVIADEVFLPYPLEDP
                     AEPSFARETEVPCFVLNGLSKLLGLPQMKLGWIVLAGPASYRADAAGRLEIIADTYLS
                     TGAPILNAAGELLPLAAGFTEELRERLLSNLGCLREALEGEGSPYRVLRCSGGWTAVL
                     ELPRVLPEEDLALGLLREEGVFAHPGYFFDFPREAFLALSLLPRPEDFREGTGRLRAF
                     LDRLLA"
     gene            3733..6015
                     /locus_tag="P5313_14100"
     CDS             3733..6015
                     /locus_tag="P5313_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY81544.1"
                     /translation="MAVRVPIGTRIFLTLVILFAVITVGIGILVSFTLVGTVERAVVG
                     NIGRDTSLLAKDFETWLAEKYQILETLKHSVLQNRNDPEALLDILIAKTREEPDIAWI
                     YYGTETFDPRFTPVKDGVTRTANGGFYVDADEWNPDPDFIWPSRPWFVRARDENQAVM
                     SDPYIDANTKEIVVSLSRSVRDEGGRLLGVIALDMYISRLTEVVSVQKYTPSSKTYLI
                     DAKGSFITHEDKSLVLKLDDPAGNLFSPGTPLAAMNEAILGTPETWSRNRSKNLYWAA
                     RRIPRTSWTVVTYGKYSDIARPVVEFYGTLLGIMGAAILMAVLFALAEARAISAPIEQ
                     LKRGALEMAKGNLSWNVQIKSRDEFGELGDFFNQVSASLKENISRIEGQRQEIEDYSK
                     NLEKMVEERTRQLDHANKELTARNVQMEKEMQMAEVVQRKTIPQTVDLPAAPELRFGA
                     RYVAMQAVGGDLYDVLPIDGRRYALLIADVSGHGMAAALVAAMAKVAFRTQAGIGLGP
                     AEVCRAVNQEIHEIIGGETYYLSAFYLVLDVVDGTLTYSNAGHPPVLLVRKDRTIEKL
                     DVAGGQLLGISGDFAFEEGRTRMAPGDFLLMFTDGIVEARDPDGDFYEYGRLMEYVRG
                     NRDAKPEEFIQGLLDDVDAFSRGAARSDDRAALYAQFNGYRSEATAAIAQTGDWAARF
                     DRAGRLLKSGDARSASIVLEEIRAQRPEDPRVLNMLGLASFRLGDRERAERLLETAVL
                     LAPESPTYRKNLEMVRSGKA"
     gene            complement(6047..>7235)
                     /locus_tag="P5313_14105"
     CDS             complement(6047..>7235)
                     /locus_tag="P5313_14105"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY81545.1"
                     /translation="VVAVGKLGFPDLAEAALRDGSCDMVMLGRPLLADPEWPNKAFAG
                     QVDRIRPCIGDQEGCLGELVEGGRPGCAVNPRTGFEDRLDGSELAPARNPGRVAVVGA
                     GPAGITAALAAARRGHRVTLFEARTEIGGMLVPGSRPAIKFEVANYLEWLRGEVERAR
                     AGGNLSLELGRAPTAMELKAARFDAVVTATGSRQVRPGVPGIETAGVVAAVELLRDPG
                     RAAGARRAVVVGGGTVGCETALWLSRELGLAEVSLVEMLPWLMKGVCTANRGWLIHHL
                     EEAGVRLWNCARLAEVRTGEVRVIRNVSRTVPDPRVTWEPLLPENVPNPFAREIRVEV
                     REESLGADLVVLAAGAKADDGLYRECLGTCAAPAVRNVGDSHIPGRVLEAVRSGYAAG
                     ISI"
BASE COUNT         1228 a         2471 c         2458 g         1078 t
ORIGIN      
        1 cgcgacacgc aggaagttct tggccgccgc ccggagcatg caggggcccc cgatgtcgat
       61 gttctgccgg gcagcctcga agggacccga ggccgaggag acggagccga agggatagag
      121 gttgcccacc accagatcga aggccacggc ccccgaccgg gcgcggtccg cggcgtgctc
      181 cgggtcgtag ggttccgaga gcagtcccag gtagatccgc cagtccaggg tcttgaccag
      241 cccgcccttc atctcgggct gtccggtata gtcctccatg gagacaagaa tcctctcggc
      301 cgcgggcccg agaatggtcc ggagggccga cagggtccct ccggtggaat agatacggat
      361 tccggggcag gactcgacca ggccccgggc cagatccgga aggccgatct tgtcgtagac
      421 cgacacgatc gcgttgcgaa cgggcacccg gtcgtcgatc cgggagacat ggttgaaggg
      481 catgggaggc tcctgtaagg actcagggac caggactcag gtatcggcaa gacgactgcg
      541 gattgaaacc gtcaaccgcc gcctggaaac cattccgaat cctgacgccg gactcctttt
      601 acccgtactc cacatacgcg tacgcgctgt ggttgtggat gctctcgaag ttctccgcct
      661 ccacggaaaa gcccttgaag atccccatcc cgtcgatgcc gcgaagggcg tcccgcacca
      721 cgtcctccac gaagcgcggg ttgtcgaagg ccttctcggt cacgtacttc tcgtcgtccc
      781 gcttgaggag ggtgtagacg tcgcaggagg ccgcggactc gatcacggcg atgaggtcct
      841 cgagccagaa gaaggggccg acctccaccg tcgcggtgac cacgcccctc tggttgtggg
      901 ccccgtgggc gctgatggcc ttcgagcaag ggcacacggt ctgcacgggg acggacacgg
      961 agacccggaa ctgccgtacc tggtcggaaa cccggccgga atagacgcag tcgtagctca
     1021 tcacgctctt ggcgccggag accggggccg ccttctcgat gaagtacggg aaatgtatgt
     1081 ccgagtaggc ggtctcggcg tccagggcct gccggatctc ccccagcatc cccaggaacc
     1141 tcggcatgga caggtcttcc cggtgggcgt tgaagacctc gatgaagcgg ctcatgtggg
     1201 tgcccttgaa gtcgtggggg aggtccgcgt agaggttggc gaccgcggtg gtccactggg
     1261 tgccgcgctc cttgtcccgg agcatgatgg ggtaccggag gcccttgacg ccgaccttct
     1321 ggatggggat gcggcgctcg tcccgcatgg actggatgtc gatcatgccc ggacggtccg
     1381 ctccgcggat tccacggtgt tggacaggag catggcgatg gtcatgggac cgacgccccc
     1441 ggggacgggg gtgatggaac ccgcgaccgg cagggcggac tggaagtcca cgtccccgac
     1501 caggcggtag cccttggccg cggagaggtc ctccacccgg ttgaccccca cgtcgatgac
     1561 gcaggccccg ggccggatca tgtcccccgt gatcagccgg ggccggccgg cgcaggcgat
     1621 caggatgtcc gcccggcggg tatgctccgc gagatccctc gtgccggtgt ggcagacggt
     1681 caccgtggag ttgagctcct tgcggacaaa gaggttggcc aggggcttgc ccacgatgtt
     1741 ggaccgtccc acgacgacca cgtgggcgcc cttggtgggc accccggccc gttcgaccag
     1801 cttgaggatc ccgtgggggg tgcaggggag aaagcagggc tgtcccagga tcatccgtcc
     1861 gacgttgacg ggggtgaagc cgtccacgtc cttgtccggg gatatggcgg ctatgacggc
     1921 atcctcgtcg atgtggtccg ggaggggcag ctggaccagg atgccgtgga ccgcgggatc
     1981 ccggttgaag gtttccacca gggcgacgag ctccggctgg ggggtcgagg ccggcaggcg
     2041 gcgctcgaag ctccgcatcc ccgcctcctc gcaggccttt tccttggagg tgacgtagga
     2101 gacgctggcg gggtcctccc cgaccaggat gacggccaat ccggggacca ccccccgggc
     2161 cgcgagggcg gaggccctcc ttttcacgtc ctcccggata tccgcggcca gggcctttcc
     2221 gtcgatgatc gaagctgcca tggaacctcc ccggggacgg cggcgcgggc atgcgggagg
     2281 ccgggtcccc tggacgtata gtacctacgc gggcccgtcc gggcaagcgg agggcccggg
     2341 tccggaggac ggcgcggctt ccggtctatg ccaggagcct gtccaggaag gcccggagcc
     2401 gcccggttcc ttcccggaaa tcttccggcc tcggaaggag gctaagggcc aggaaggctt
     2461 cccgcgggaa gtcgaagaag tatccgggat gcgcgaagac cccctcctcc cgcaggagcc
     2521 cgagggccag atcctcctca ggcaggaccc tcggaagctc caggaccgcg gtccagcctc
     2581 cggaacagcg gagaacccgg tagggactgc cctccccttc cagggcttcc cggaggcagc
     2641 cgaggttgga gaggagccgt tcccggagct cctccgtgaa gccggcggcc agggggagca
     2701 gttccccggc ggcgttcagg atgggagccc ccgtcgagag gtaggtgtcc gcgatgatct
     2761 ccaggcgccc ggccgcgtcc gcccggtacg aggcgggacc ggccagtacg atccacccca
     2821 gcttcatttg gggaagcccc aggagtttgg acaagccgtt caggacgaag cagggcacct
     2881 ccgtttcccg cgcgaaactc ggctccgccg gatcctccag gggatacggc aggaagacct
     2941 cgtccgcgat gaccgccgtc ccggcctccc cgcagaggtc caggattcgg cgccgctccc
     3001 gacggccgac gaaggacccc gtcgggttgt tcgggtggat gaggatgacg gccttcggcc
     3061 tcctcgactt cagggcggct tccagggaat ccaggtcggc gctccagccc gcggggtggc
     3121 ggtattccag gggatacggg accgcctcca cgccctccag tcccgacagg tactcgaaca
     3181 gggggtagcc gggcttaggc accaggacgg agtcccccgg gtccgcgaga agtttgaaga
     3241 gccagccgta ggcctcggag gtgctggccg ccaggaagat atcggaggga tcgaccgacg
     3301 ttcctcggga ggcgtaccag gaagccacgg cggatcgggc cgcgggcagg ccccgggggt
     3361 ccggctcgta ggagaggctc tcggtccggg cgagggcccg gagcaccttc tccgcgggca
     3421 gggcggcgcc gacccgagtg ggattgcttt ccgccaggtt gatcaccctc cggcccgagg
     3481 cctgggcttc ggctaaggcc cgtcccagtg cgttgggctc ccggggggcg ggaatacggc
     3541 gggagaaggg agggaacggc atgggggctc cttgaggcga caggactcag gacccagtga
     3601 caagatagac gacagtggat tccaataatt cgtcggtcgt catacaggtc tcccggctcc
     3661 tgactcctga ccactgtctc ccacagtatg ctataatcct ccccaatacc agcttcccac
     3721 agggagtccg ccatggccgt ccgagttccc atcggcacca gaatcttcct caccctcgtg
     3781 atcctgttcg cggtcatcac cgtgggcatc ggtatcctgg tttccttcac cctggtcggc
     3841 accgtggagc gggccgtggt gggaaacatc ggccgggata cgagcctgct ggccaaggac
     3901 ttcgagacct ggctggcgga gaagtaccag atcctggaaa ccttgaaaca ctcggtgctc
     3961 cagaaccgaa acgatccgga agccctcctg gacatcctga tcgccaagac ccgggaagag
     4021 ccggacatcg cctggatata ctacggaacc gagaccttcg acccccggtt cacccccgtc
     4081 aaggacggtg tgacccggac ggccaacggg ggcttttacg tggacgcgga cgagtggaac
     4141 ccggaccccg atttcatctg gccctcccgc ccctggttcg tccgggcccg ggacgaaaac
     4201 caggccgtca tgagcgaccc ctacatcgac gccaacacca aggagatcgt ggtctccctg
     4261 tcccgttcgg tacgggacga gggcggcagg ctcctggggg tgatcgccct ggatatgtac
     4321 atctcgaggt tgacggaagt ggtgtccgtc cagaagtaca ctccctcctc caagacctac
     4381 ctgatcgacg ccaagggatc cttcatcacc cacgaggaca aatccctggt cctaaagctg
     4441 gacgacccgg cgggcaacct gttctccccg ggcacccccc tggcagccat gaacgaggcc
     4501 atcctgggaa ccccggagac ctggagccgc aatcggagca agaatctcta ctgggcggcc
     4561 cggcgcatcc cccggaccag ctggaccgtc gtcacctacg gcaagtactc cgacatcgcc
     4621 cggccggtgg tggaatttta cggaaccctg ctggggatca tgggcgcagc catcctgatg
     4681 gcggtactgt tcgctctcgc ggaggcccgg gccatctcgg ctcccatcga gcagctcaag
     4741 cggggcgccc tggaaatggc caagggcaac ctctcctgga acgtgcagat caagagccgg
     4801 gacgagttcg gggagctcgg ggatttcttc aaccaggttt cggcttccct caaggagaac
     4861 atctcgcgta tcgaggggca gagacaggag atcgaggact actcgaagaa ccttgagaaa
     4921 atggtcgagg agaggacccg gcagctggac cacgccaaca aggaactgac cgcccggaac
     4981 gtccagatgg agaaggagat gcagatggcg gaggtggtcc agcggaagac catcccccag
     5041 accgtggatc tgcccgccgc gccggagctc cggttcggcg cccggtacgt ggccatgcag
     5101 gccgtggggg gggacctcta cgacgtcctg cccatcgacg gacgccgcta cgccctgctc
     5161 atcgcggacg tctcgggaca cggcatggcc gccgccctgg tggccgccat ggccaaggtc
     5221 gcattccgga cccaggccgg catcggcctc ggccccgcgg aagtctgccg ggcggtcaac
     5281 caggagatcc acgagatcat cggcggggag acctactacc tctccgcctt ctacctggtc
     5341 ctggacgtgg tggacggcac cctgacctat tccaacgccg gccatccccc ggtgctcctg
     5401 gtccggaagg accggacgat cgaaaagctg gacgtggccg gaggacagct cctggggatc
     5461 agcggggatt tcgccttcga ggaaggccgc acccggatgg ccccgggcga cttcctgttg
     5521 atgttcaccg acggcatcgt ggaggcccgg gaccccgacg gggacttcta cgagtacggc
     5581 aggctcatgg agtacgtccg gggaaaccgg gacgccaagc cggaggagtt catccagggg
     5641 ctcctggacg acgtggacgc gttcagccgc ggagccgccc gatcggacga ccgggccgcc
     5701 ctgtatgcgc agttcaacgg gtaccggtcg gaggcgaccg cggcgatcgc ccagaccggc
     5761 gactgggccg cccggttcga ccgggccgga cgcctgctca agtccgggga cgcccggtcc
     5821 gcctcgatcg tcctggagga gatccgggcc cagaggcccg aggatcctcg ggtcctgaac
     5881 atgctcggcc tggccagctt ccgcctgggc gaccgggaac gggcggagcg gctcctggag
     5941 accgcggtcc tcctggcccc cgagtccccg acgtaccgga aaaacctgga gatggtccgg
     6001 tcgggcaagg cctagggggc cgatcgggcg aagccgcgcc gatcccctag atggagatcc
     6061 ccgccgcgta tccggaccgg accgcctcca atacccggcc ggggatgtgg gagtccccga
     6121 cgttccgcac ggccggagcg gcgcaggtcc cgagacattc ccgatacagc ccgtcgtccg
     6181 ccttggcccc cgcggccagg accacgaggt ccgcccccag ggactcctcc cgcacctcga
     6241 cacggatctc ccgggcgaag ggattgggca cgttctccgg caggaggggc tcccaggtca
     6301 cccggggatc cggcacggtc cgggacacgt tccgtatcac ccgtacctcc ccggtccgga
     6361 cctccgcaag acgggcgcag ttccagagcc ggacccccgc ctcctccagg tggtggatca
     6421 gccagccccg gttggccgtg cagaccccct tcataagcca ggggagcatc tccacgaggg
     6481 aaacctcggc cagtcccaac tcccgggaaa gccagagggc cgtctcgcag cccacggtcc
     6541 cgcccccgac gacgacggcc cgccgggcac cggccgccct gcccggatcg cgcaggagct
     6601 ccaccgccgc gaccaccccg gccgtctcga tccctgggac cccgggccgg acctgccggg
     6661 accccgtggc cgtcacgacc gcgtcgaacc gggccgcctt gagttccata gccgtcggcg
     6721 cccggcccag ctcgagggag aggttccccc cggcccgggc ccgctcgacc tccccgcgaa
     6781 gccattccag gtagttcgcc acctcgaact tgatcgccgg tcgggatccg ggcaccaaca
     6841 tacccccgat ctccgtgcgg gcttcaaaca gggtgacccg gtggccccgc cgggccgcgg
     6901 ccagggccgc ggtgatcccc gcgggtcccg cgcccacgac ggccacccgg cccggattcc
     6961 gggccggggc cagctccgac ccgtccaggc ggtcctcgaa gcccgtccgg ggattgacgg
     7021 cgcagccggg ccggccgcct tccacgagct ccccgaggca gccctcctgg tccccgatgc
     7081 aggggcggat ccggtccacc tgccccgcga aggccttgtt cggccattcc gggtccgcca
     7141 ggagggggcg gcccagcatg accatgtcgc aggagccgtc ccggagggcc gcctccgcca
     7201 ggtccgggaa accgagcttt ccgacggcga ccacg
//
LOCUS       DAOTEI010000179         7223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7366_length_7223_cov_5.323940, whole genome shotgun sequence.
ACCESSION   DAOTEI010000179 DAOTEI010000000
VERSION     DAOTEI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7366_length_7223_cov_5.323940"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2182)
                     /locus_tag="P5313_14110"
     CDS             complement(<1..2182)
                     /locus_tag="P5313_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY81546.1"
                     /translation="MFLKSLEILGFKSFADRTRIEFSPGISALLGPNGCGKSNVVDSI
                     KWVVGEQSARSLRAESMEDIIFNGTETRKALNVAEVTLTISNENSSLALDAPEIAIKR
                     RLYRSGESEYFLNGQAVKLKEIRELFWDTGIGKSAYSVMEQGRIDQILSSKPEERRYL
                     FEEAAGITKHKVRSREAELKLERTEENVRQVQGILGEVKRSHDTLKSQSEKTLAYRAL
                     RDDVFQAELDLHLLRLRGFVQDKARRDEEAADKVRARDAVKKSIDDINLNLEESLDVV
                     NTMESKLVEHQKTVYGLAVERAGKEKQHKLLAERVSEAKAKIDQARLRRKGAQERLEG
                     LQEEAEEKEGEVRSLKARILEIEKNIASFESNITAADGRIRDNEAAILRSEKGIADLE
                     AGQAQAQRELDALTDDIVRELDARLKETGYSAKERRAAEEAIDGLIAAVHARLAGKEA
                     LLSDLGRLSDYKAEDARALIEKARADLEGTKARIEELRGLYEAYKRTTPSFIDEFLSP
                     EGIITQKRAVDERIARIKDEIAALKEKILALREENRQLAERIDEYRRTLEELRGNRIR
                     MQTQGQAAEEALTLLKREVAVQEGVLKELENEVYLEEKRLADATEQLADLDQEIAEIE
                     TKGRRLTAEMEKLERDIALKNSDLGKRQEDLRKKMDQLGRFQAELERIHLTLAQTDTE
                     IRNIKDNFRETYSRDLMEFEERMFEIRVPVQELRDRLQERRTKLK"
     gene            2421..3080
                     /locus_tag="P5313_14115"
     CDS             2421..3080
                     /locus_tag="P5313_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRY81547.1"
                     /translation="MLYPDPEEALRDLRGAALESAYRAWAKRLHPDGPDGNHEAFLRL
                     RKAYEEAKTGGPRPSAPDPEAFDPWEAVRETGRRGPLSPEDCLFAVLERWRQAGSPTA
                     KPVASPSTGARNRRILRSVLHWARAVDPAFARDFALLVRFDAGPLRTTATSRAEREAR
                     RKLEAGLDDLLRWREEGRKVTAELARERLEAAVELVELHGLPRPEITRIAEWMIGKVN
                     G"
     gene            complement(3168..4400)
                     /gene="kbl"
                     /locus_tag="P5313_14120"
     CDS             complement(3168..4400)
                     /gene="kbl"
                     /locus_tag="P5313_14120"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRY81548.1"
                     /translation="MYGAMKDDLRKKLDAIRAEGLYKAERVIATPQGARIRVRGGKEV
                     LNFCANNYLGLANHPAIREASRKAQDEWGYGLSSVRFICGTQEPHKLLEKAAADFLGT
                     EDAILFSSCFDANGGVFEPLLDEDCAIITDSLNHASIIDGVRLCKAKRWIWKHGDLRD
                     TEEIDPDTGKPAKGLERCLKEAASCRYRMVATDGVFSMDGDIADLASVCDLAEKYGAL
                     VMVDDSHATGFVGRTGRGTPEHCGVSGRVDILTTTFGKALGGASGGVVAARAEIVEYL
                     RQKARPYLFSNTLAPGIVGGTLAALDLLSGSTELRDRLEANTRRFREGMTAAGFDIRP
                     GVHPIVPVMLYQEKLAHDMADRLLDEGIYVIGFSFPVVPRGKARIRVQISAAHSDADI
                     DRALEAFRKVGREMGVVK"
     gene            complement(4503..4769)
                     /locus_tag="P5313_14125"
     CDS             complement(4503..4769)
                     /locus_tag="P5313_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81549.1"
                     /translation="MRERPLRVRFCGDCERMILVDFKFCPHCGSPVRPVVPPAEPPVP
                     EAREGKAPIPRDGREARVLLDRLIRELEVLDAEMVEWTAVNGKR"
     gene            complement(4766..6076)
                     /locus_tag="P5313_14130"
     CDS             complement(4766..6076)
                     /locus_tag="P5313_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRY81550.1"
                     /translation="MSSGNDIHKTFVIDTNVLIHYPEAIMSFKDNEIVLPLWVLEELD
                     TLKSYQDQRGKSAREAIRFLDSLIKKGNLHDGVKLDNGSVLRVSLRLPAETPEGVELE
                     KTDNRIILCAYGLQQEGRKVFFVSKDINARVKATALGLKAVDYEKQKVDIASLYPGVR
                     DVDASEEMLKTLAETGSLPWKERLRANEFVVLHDRAGHRERVLRYDPDAEFLRSSDPE
                     SPSASGITPLNERQRIALELLLDDSVPLVTLVGKAGTGKTLLAIAAGLRMVYDEKKYT
                     RMLVSRPVVPMGKDIGYLPGEKQAKLANWMQPLFDNLEFILSAHKRPNVKNVDQLIKD
                     KQIEIEALTYIRGRSLPGQYIIVDEAQNLTPHEIKTIVSRAGDGTKVVLTGDPYQIDN
                     MYLDANSNGLSFLVEAFKGQRLYGHVTLLRSERSQLAELAASLL"
     gene            complement(6128..6688)
                     /locus_tag="P5313_14135"
     CDS             complement(6128..6688)
                     /locus_tag="P5313_14135"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY81551.1"
                     /translation="MRIKYNAPTTLTFTLLCAVVLALDTLLAPSLAETWFSAPGRGAF
                     RADSARDFIRLVTHVLGHADLNHFLSNFAFILLLGPMLELAYGSLTLFIMILITSLVT
                     GVLNTLLFPVSLMGASGVVFMMILLSSFTNFGKGEIPLTFVLIMVLYLGREVLSSFQR
                     NSVSEFAHIIGGLCGSLFGYFRPARR"
     gene            complement(6685..>7223)
                     /locus_tag="P5313_14140"
     CDS             complement(6685..>7223)
                     /locus_tag="P5313_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRY81552.1"
                     /translation="SVSFLGVREFDEEGRVLAADFGGLTVISAYFPNSQDGGARLGYK
                     LGFCKAIREFCDGITARGGHIVLSGDYNIAHTPIDLARPKENEENPGYLPEERAWMDE
                     FTGAGYVDTFRVLHPGEPGHYSWWSYRTRAREHNVGWRIDYHCVDPGIRTRIRSAAIR
                     SDVTGSDHCPVTLELEEA"
BASE COUNT         1122 a         2354 c         2393 g         1354 t
ORIGIN      
        1 ccttgagctt ggtcctgcgc tcctggaggc ggtcccgcag ttcctggacg gggacgcgga
       61 tctcgaacat ccgctcctcg aactccatca ggtcccggga gtaggtttcc cggaagttgt
      121 ccttgatgtt gcggatctcc gtgtccgtct gggccagggt caggtggatg cgctccagct
      181 ccgcctggaa gcggcccagc tgatccatct tcttccggag atcctcctgg cgcttgccca
      241 ggtccgagtt cttgagggcg atgtcccgct ccagtttttc catctccgcg gtcaggcggc
      301 ggcccttggt ctcgatctcc gcgatctcct ggtccaggtc ggccagctgt tccgtggcgt
      361 ccgccaggcg cttctcctcg aggtagacct cgttctccag ctccttgagg accccctcct
      421 ggacggcgac ttcccgcttg aggagggtca gggcctcctc cgcggcctgt ccttgcgtct
      481 gcatccggat ccggttgccc cggagctcct ccagggtcct gcggtattcg tcgatccgct
      541 cagccagctg gcggttctcc tccctcaagg ccaggatctt ctccttgagg gccgcgatct
      601 cgtccttgat ccgggcgatc cgctcgtcca cggcccgctt ctgggtgatg atgccctcgg
      661 gggagaggaa ctcgtcgatg aaggaggggg tcgtccgctt gtaggcctcg tacaggccgc
      721 gcagctcctc gatccgggcc ttggtgccct ccaggtcggc ccgggccttc tcgatcaggg
      781 cccgggcgtc ctcggccttg tagtccgaga gccggcccag gtccgaaagg agggcttcct
      841 tgcccgccag ccgggcgtgg accgccgcga tgaggccgtc gatggcctcc tcggcggccc
      901 ggcgctcctt ggcggagtac cccgtctcct tgagacgggc gtccagttcc cggacgatgt
      961 cgtccgtgag ggcgtccagc tctctctggg cttgagcctg accggcctcc aggtcggcga
     1021 tgcccttctc ggagcgcagg atggccgcct cgttgtcccg gatgcggccg tccgcggcgg
     1081 tgatgttgga ttcgaaggag gcgatgttct tctcgatctc caggatccgg gccttgaggc
     1141 tgcggacctc cccttccttc tcctcggcct cctcctggag accttccaga cgctcctggg
     1201 cccccttgcg gcgcaggcgg gcctggtcga tcttggcctt ggcctcggag acccgctcgg
     1261 ccaggagctt gtgctgcttc tccttgcccg cccgctccac cgccaggccg tagacggtct
     1321 tctggtgctc cacgagcttg gactccatcg tgttgacgac gtccaggctc tcctccagat
     1381 tcaggttgat gtcgtcgatg gatttcttga ccgcgtcccg ggcacggacc ttgtccgcgg
     1441 cctcctcgtc ccggcgggcc ttgtcctgga cgaagccccg gagccggagg aggtggaggt
     1501 ccagctcggc ctggaacacg tcgtcccgca gggcccgata agccagggtc ttctcggatt
     1561 gggacttgag ggtgtcgtgg ctgcgcttga cctcgcccag gatgccctgg acctggcgta
     1621 cgttctcctc cgtccgctcc agtttgagct ccgcctcccg ggaccgcacc ttgtgcttgg
     1681 tgatgccggc cgcctcctcg aagaggtagc gcctctcctc gggcttggag gagaggatct
     1741 ggtcgatgcg gccctgctcc atgacggagt aggcgctctt cccgatcccg gtgtcccaga
     1801 agagctcccg gatctccttc agcttgaccg cctggccgtt caggaagtac tcgctctccc
     1861 cggagcggta gaggcggcgc ttgatggcga tttccggggc gtccagggcc agggaggagt
     1921 tctcgttgga gatggtcagg gtcacctcgg cgacgttgag agccttccgg gtctccgtgc
     1981 cgttgaagat gatgtcctcc atgctctcgg cgcggaggct gcgggcggac tgctccccca
     2041 ccacccactt gatggagtcc acgacgttgc tctttccgca gccgttggga cccaggaggg
     2101 cgctgatgcc cggggagaac tcgatacggg tgcggtcggc gaagctcttg aaacccagta
     2161 tttccagact cttgaggaac acgaataccc tcggggcggt cgattatcgt atgcgcgggc
     2221 cgggccggcc ggcggggcgc gcgtccggaa atactagcac gtgaggcgtg aataggtgaa
     2281 tgggtgaata ggtgaataag agcggtattg tcgatcgcgg ttacataaat ccccaggcgg
     2341 atacgcaggg gtacgacgcc gacttcccga ttcactattc accaattcac tattcaccca
     2401 ttcactacta tacttccccc atgctgtacc ccgacccgga ggaggccctc cgggacctgc
     2461 gcggagcggc cctggagagc gcctaccggg cctgggccaa gcgcctgcat cccgacgggc
     2521 cggacgggaa ccacgaggcc tttctccggc tccggaaggc ctacgaggag gccaagacgg
     2581 gcggaccccg accttcggcg cccgatccgg aagccttcga cccctgggaa gcggtccggg
     2641 aaaccggccg gcggggcccc ctgtccccgg aggactgcct cttcgcggtc ctggagcggt
     2701 ggcggcaggc gggctccccc acggccaagc ccgtggccag cccctccacg ggcgcccgga
     2761 accggaggat cctccgctcg gtcctgcact gggcccgggc cgtggacccg gccttcgccc
     2821 gggacttcgc cctcctggtg cgcttcgacg cgggccccct aaggaccacc gcgacctccc
     2881 gggcggagcg ggaagcccgg cggaagctgg aagccgggct ggacgacctc cttcgctggc
     2941 gggaggaggg gcgcaaggtc acggccgagc tcgcccggga gcgcctggag gccgccgtgg
     3001 agctcgtgga gctccacggt ctgccccggc cggaaatcac gcggatagcg gagtggatga
     3061 tagggaaggt gaatgggtga atacgtgaat gggcgaatag gaaaagcgga acaaaacgat
     3121 aaatatcgta ccggattcac ctattcacta ttcaccaatt cactcgttca cttcacaacg
     3181 cccatttctc tccccacctt ccggaacgcc tccaaggccc ggtcgatgtc cgcgtccgaa
     3241 tgggcggcgg agatctggac ccggatgcgc gccttgcccc ggggaacgac ggggaaggag
     3301 aatccgatca cgtagatgcc ctcgtccagg aggcggtccg ccatgtcgtg ggccagcttc
     3361 tcctggtaga gcatcaccgg gacgatgggg tggacccccg gacgtatgtc gaagcccgcg
     3421 gcggtcatgc cctcccggaa gcggcgggtg ttggcttcca gccggtcccg gagttcggtg
     3481 ctgccggaga ggaggtccag ggccgccagg gtccccccca cgatgcccgg ggccagggtg
     3541 ttggagaaga ggtagggccg ggccttctgt cggaggtact ccacgatctc cgcccgggcc
     3601 gccacgaccc ctcccgaggc ccctcccagg gccttcccga aggtggtggt caggatgtcc
     3661 acccgccccg acaccccgca gtgctccgga gttccccggc cggtccggcc cacgaaaccc
     3721 gtggcgtggg aatcgtccac catgaccagg gctccgtatt tttccgccag gtcgcagacc
     3781 gaggccaggt ccgcgatgtc cccgtccatg gagaacaccc cgtcggtggc caccatacgg
     3841 tacctgcagg aagcggcctc ctttaggcag cgctccaggc ccttggcggg cttgcccgtg
     3901 tcgggatcga tctcctccgt gtcccggagg tccccgtgct tccagatcca gcgcttggcc
     3961 ttgcacaggc gcaccccgtc gatgatggag gcgtggttca agctgtccgt gatgatcgcg
     4021 cagtcctcgt ccaggagggg ttcgaaaacg cctccgttgg cgtcgaagca ggaggaaaag
     4081 aggatcgcgt cctcggtgcc caggaagtcc gccgccgcct tctccagaag cttgtggggc
     4141 tcctgggtcc cgcagataaa ccggacgctg gaaaggccgt agccccactc gtcctgggcc
     4201 ttccgggaag cctcccggat cgccggatgg ttggccaagc ccaggtaatt gttggcacag
     4261 aaattgagaa cttccttgcc gccccgcacc cggatccggg caccctgggg cgtggcgatc
     4321 acacgttcgg ccttgtagag cccctccgcc cggatggcgt ccagcttctt cctgagatca
     4381 tccttcatag cgccgtacat ggttcctccc ggataggact tgggtttctg agtttaggtt
     4441 cgtgggggat tccatagtat cacacttcgg cgtccggtgt gcttccttcc actgtccacc
     4501 gctcaccgtt taccgttcac cgccgtccat tcgaccatct ccgcgtccag gacctcgagt
     4561 tcccggatca gtcggtccag gaggacccgg gcttcccggc catcccgggg gataggggcc
     4621 ttcccctccc gggcctccgg aacgggaggt tcggcgggcg gaacgaccgg ccggaccggg
     4681 gacccgcagt ggggacagaa tttgaagtcg accaggatca tgcgttcgca atccccgcag
     4741 aaccggaccc tcagggggcg ttccctcata ggaggctcgc ggccagttcg gccaattgac
     4801 tgcgctcgct ccggagcagg gtgacgtgcc cgtacaggcg ctgtcccttg aaggcctcca
     4861 ccaggaagga gaggccgttg gagttggcgt ccaggtacat gttgtcgatc tggtagggat
     4921 ccccggtcag cacgaccttg gtcccgtccc cggcccggga gacgatcgtc ttgatctcgt
     4981 ggggggtcag gttctgggcc tcgtccacga tgatgtactg gcccggcagg gaccggcccc
     5041 ggatgtaggt cagggcctcg atctcgatct gcttgtcctt gatcaactgg tccacgttct
     5101 tgacgttggg ccgcttgtgg gcggagagta tgaactccag gttgtcgaag agaggctgca
     5161 tccagttggc cagcttggcc tgcttctccc cggggaggta gccgatgtcc ttgcccatgg
     5221 gcaccacggg gcgagagacc agcatgcggg tgtacttctt ctcgtcgtag accatgcgca
     5281 ggcccgccgc tatggccagg agggtcttgc ctgtgccggc cttgcccacg agggtgacca
     5341 ggggaacgga gtcgtccagg aggagctcga gggcgatccg ctggcgctcg ttcaggggcg
     5401 tgatgcccga ggcggagggg gactccggat ccgaagaccg gagaaactcc gcgtccggat
     5461 cgtaccggag gacccgttcc cggtgcccgg cgcggtcgtg gaggacgacg aactcgttgg
     5521 cccgcaggcg ctccttccag gggaggctcc cggtttccgc cagggtcttc agcatctcct
     5581 ccgaggcgtc cacgtcccgg accccggggt agagggaggc tatgtccacc ttctgcttct
     5641 cgtagtccac ggccttgagc cccagggcgg tggccttgac ccgggcgttg atgtccttgg
     5701 agacgaagaa gaccttccgt ccctcctgct ggagcccgta ggcgcagagg atgatccggt
     5761 tgtccgtctt ctccagttcc accccctccg gggtctccgc ggggagccgc agggagaccc
     5821 ggagcacgga gccgttatcc agcttcaccc cgtcgtggag gttgcccttc ttgatgaggg
     5881 agtccaggaa ccggatggcc tcccgggcgc tcttgccccg ctggtcctgg taggacttca
     5941 gggtgtccag ctcctccagg acccagaggg gaagcactat ctcgttgtcc ttgaaggaca
     6001 tgatcgcttc cgggtaatgg attaggacat tggtgtcgat gacgaaggtt ttatggatgt
     6061 cgttgccgga actcatgaac tcaagtattc ggcacgaccg ggagaaacgc aagggttccg
     6121 gtagaggtta tctcctcgcg gggcggaagt acccgaagag gctcccgcag agtcccccga
     6181 tgatgtgggc gaactcggat acggagttcc gctggaagga cgacaggact tcccggccca
     6241 ggtacaggac catgatcagc acgaaggtca gggggatctc ccccttgccg aagttcgtga
     6301 acgaggatag gaggatcatc atgaagacca cgccgctggc tcccatgagg ctcacgggaa
     6361 ataggagggt gttgaggacc cccgtcacga ggctggtgat caggatcatg atgaagaggg
     6421 tgagggagcc gtaggccagc tccagcatcg gccccaggag caggatgaag gcgaagttcg
     6481 aaaggaagtg gttcaggtcc gcgtgcccca ggacgtgggt gaccaggcgg atgaagtccc
     6541 gggcggaatc cgcccggaag gcgccccggc cgggggcgga gaaccaagtc tccgccaggg
     6601 acggggccag gagggtgtcc agggccaaga ccacggcgca cagaagggtg aaggtcaggg
     6661 tggtgggcgc gttgtacttg attctcatgc ttcctccagc tccagcgtca cggggcagtg
     6721 gtcggacccg gtcacgtccg agcgtatcgc ggcggaccgt atccgtgtcc ggatcccggg
     6781 atccacgcag tggtaatcga tcctccaccc cacgttgtgc tcccgggcgc gggtccggta
     6841 ggaccaccag gaataatgcc ccggttcccc gggatgcagg acccggaagg tgtccacgta
     6901 tcccgccccg gtgaactcgt ccatccaggc ccgctcctcg ggcaggtagc ccgggttctc
     6961 ctcgttttcc ttggggcggg ccaggtcgat gggggtgtgg gcgatgttgt agtccccgga
     7021 caggacgatg tgtcctcccc gggccgtgat cccgtcgcag aactcccgga tcgccttgca
     7081 gaagcccagc ttgtatccca gacgggcgcc tccgtcctgg gaattcggga agtaggcgga
     7141 gatcaccgtg agcccgccga aatccgcggc caggaccctg ccctcctcgt cgaattcccg
     7201 gacccccagg aaggagacgg acc
//
LOCUS       DAOTEI010000180         7130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7509_length_7130_cov_5.569753, whole genome shotgun sequence.
ACCESSION   DAOTEI010000180 DAOTEI010000000
VERSION     DAOTEI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7509_length_7130_cov_5.569753"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="P5313_14145"
     CDS             <1..392
                     /locus_tag="P5313_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY81553.1"
                     /translation="VEYVPAAPGTEDLVLTDAQKAALNSRKAFRGRTFPAVILPGDEE
                     DYGGCLAAGRGFLHLSPEGALEACPFAPYSDTGVSDRSLREALDSPLLAAIRARHSDL
                     TETKGGCALWNRGGWIASLGACAAKAG"
     gene            469..747
                     /locus_tag="P5313_14150"
     CDS             469..747
                     /locus_tag="P5313_14150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY81554.1"
                     /translation="MEWRASHILVKDRAQAQELLKRIKQGADFASLARENSTCPSKSS
                     GGDLGWFGPGKMVASFEAACKSLGVGSVSDVVQTQFGYHIVKVTGKRD"
     gene            complement(744..1991)
                     /locus_tag="P5313_14155"
     CDS             complement(744..1991)
                     /locus_tag="P5313_14155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY81555.1"
                     /translation="MQYPLPTPGQGLSLLLVGFLLAVATLHLAMYAGGRRKAGDLPFS
                     LLAVFAAFRVALIGGILDSLVSSLPSEHGRLDAVPDFLILPAFVYFLRTLFPEESDTR
                     TARAVAAASGAWLVGAFLLPREAVRWISLSYMAVAAGGLAAALAALVRAERAGRSAVR
                     QARVGTLLFGVLLVLDLLGIAAGPGPGRAGLAIGWVAIFAACSAALGRRIQDSYEEAR
                     EASRALARHNEELEVLVENRTRELTEAAEGLRRMAETDGLTGIANRRRFEAALDEERR
                     RAARTGSTLSIGMFDVDHFKAYNDTYGHLEGDECLKRVAAVLSSHARRPGDLAARYGG
                     EEFALLLPDTDPAGALVRVRSAAAEIRNLGIPHESSPFRIVTISAGVASAPRGQGDLV
                     LAADQALYAAKASGRDSVRPAET"
     gene            complement(2089..4605)
                     /locus_tag="P5313_14160"
     CDS             complement(2089..4605)
                     /locus_tag="P5313_14160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HRY81556.1"
                     /translation="MVDPSPPGPDAGAGPTAAPDPGPSGHPGFLVHGWADSRGRMLLA
                     GRLSDGRSFAVVENRVRPDFLIRSADVERASEAAARARLEASFTSSGRRTLAGEPCDR
                     TEARDRGELRRLEEAFGAAGIPVYEADVRAADRFLAERRIRGGLVIRGTPVPGRFVDA
                     VFLDPDLEAAEVPPALRTAALDIETDVEAGTVLAVSLAGPGLREVLFLDEAAGLFSPD
                     LAGADPAARNPSPFEEADDEGRTYLARPFSREADLLRALGERIRALDPDLITGWNVVD
                     FDLAHLSRRCQALAVPFRIGRTEDPADIRELDGGRRYAASIPGRQAVDALKLARGAGR
                     FEDLTLDSVAREVLGTGKSVESRGRAKIEELSRLRSGDPAAFRRYCLADSELVLAILD
                     KTGLLDLTLKRAQLTGTGIDLAWTSIPAFERIYLDELMARGYAAAPKVEGRRVSGAAG
                     GTILEPRPGLFRNVLVFDFRSLYPSVMRTFNVDPLAFRLAAAEPRPDDLEAPNGARFR
                     REPGILPAVIERYFAERLAAIERGDETASYVYKILMNSFYGVLGAEGCRYADTALAGA
                     VTSYGRKWLHWARDWFAAKGFRVLYGDTDSAFVDSGLPEGALWGALDGLGRRLAPELN
                     ADLAAAVRDEYGLESRMELKFEKVYARFFIPPLRSRTEDDEEGDEKGRAKGYAGFRLG
                     PEGGETEVKGMEAARRDGTALARELQVETMDLVFRDAPPARIEAHVRRVLADLRAGLL
                     DDRLVYRKALRRTAAAYTKVEPPHVRAARILGWTDRRGVVEYRITKAGAEPVGRETAP
                     LDYEHYAQRQLRPAVDALLSAAGMDPELIFGDRPQLELGL"
     gene            complement(4599..5309)
                     /locus_tag="P5313_14165"
     CDS             complement(4599..5309)
                     /locus_tag="P5313_14165"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY81557.1"
                     /translation="MNIPILFEDESLLAADKPAGVLSAPDRWDPDLPVAAALLSKGRG
                     RLWAVHRLDKDTSGVLLLARSEEAHRSLSRAFETRSVAKVYRALVRGVPDWAESSCDL
                     PLSPDGDALHRTIVDAHRGKPSRTAFRVLAAFRDFALVEARPETGRTHQVRVHLAALG
                     YPVACDPLYGDGKPVLLSAIKRKWKGDPFEERPLLARAALHAASVEIPHPATGLPLRI
                     EAPDPKDLRASLAQLEKW"
     gene            complement(5411..5836)
                     /locus_tag="P5313_14170"
     CDS             complement(5411..5836)
                     /locus_tag="P5313_14170"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY81558.1"
                     /translation="MDGIRLMTMVQSLWRKRASAALEGFGITLSQFLLIQLARRRGAI
                     RPSEAAEELASDRPTLTLVARKCVARGWLSRLRVKLDRRAHRLRLTGLGEELPGRIEA
                     ARVLSPEVLGDPLDVLDSEERGQFLRALDKVHRRARDLF"
     gene            6019..>7130
                     /gene="groL"
                     /locus_tag="P5313_14175"
     CDS             6019..>7130
                     /gene="groL"
                     /locus_tag="P5313_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710555.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRY81559.1"
                     /translation="MAKQLLFSEDARRRLLSGVEQISKAVKVTLGPKGRNVLLDKKFG
                     APTVTKDGVSVAKEVELEDAYENMGAQLLKEVATKTNDIAGDGTTTATVLAYSMVKEG
                     LKAVAAGIDPMGLKRGIDKAVELTVEEIKKASKEIKEKDEISHVASVSANNDMEIGSQ
                     IADAMEKVGKDGVITVEESKTMDTTIEFVEGMQFDRGYLSPYFVTNRDTMTTVFENPY
                     ILIHDKKISSMKDMLPVLEKIAQAGKPLVIIAEDVDGEALATLVVNHLRGTLNTCAVK
                     APGFGDRRKAMLEDIAILTGGEVISEELGLKLESTEIGQLGTAKTVKIDKENTTIING
                     AGKQKDIQDRIAQIKAQIEETTSDYDREKLQERLAKL"
BASE COUNT         1158 a         2540 c         2502 g          930 t
ORIGIN      
        1 tggtggagta cgtccccgcc gccccgggga cggaggacct ggtcctcacg gacgcccaga
       61 aggcggccct gaactccagg aaggccttcc gcggccggac ctttccggcc gtgatcctgc
      121 ccggcgacga ggaggactac ggcggatgcc tggccgcggg ccggggcttc ctgcaccttt
      181 ccccggaggg ggccctggag gcctgtccct tcgcccccta ctcggatacc ggagtctcgg
      241 accggtccct ccgggaagcc ctcgactctc ccctcctggc ggccatccgg gcccggcact
      301 cggatctgac ggagaccaag gggggctgcg ccctgtggaa ccggggcggg tggatagcct
      361 ccctgggcgc ctgcgccgcc aaggccggat gacggccttt cttcggtacg ccgatcttgg
      421 tatattgaac ccgtccccta ccctggaaac catctcaagg agcgaactat ggaatggaga
      481 gccagccaca tactcgtcaa ggaccgggcc caggcccagg aacttctcaa gcggatcaag
      541 cagggggcgg atttcgcctc cctggcccgg gagaactcca cctgcccgtc caagtcctcg
      601 ggaggcgacc tcggttggtt cggccccggc aagatggtgg cctccttcga ggcggcctgc
      661 aagtccctgg gcgtcggctc cgtgtccgac gtggtccaga cccagttcgg ctaccacatc
      721 gtgaaggtga cgggcaagcg ggactaggtt tccgccgggc ggacggagtc ccggccggaa
      781 gccttggccg cgtagagggc ctggtccgcc gccaggacca ggtctccctg cccccggggc
      841 gcggacgcca cccccgcgct gatggtgacg atccggaagg gggagctctc gtggggtatg
      901 cccaggttgc ggatttccgc agccgcggag cggacccgga ccagggctcc cgcggggtcc
      961 gtgtccggaa ggagcagggc gaactcctcc cccccgtacc gggcggccag gtccccgggc
     1021 cggcgggcgt gggaggagag gacggccgcg acccgcttga ggcactcgtc cccctcgaga
     1081 tggccgtagg tgtcgttgta ggccttgaag tggtccacgt cgaacatccc gatggacagg
     1141 gtcgagcccg tccgggcggc ccggcgccgt tcctcgtcca gggccgcctc gaatcgcctc
     1201 cggttcgcga ttccggtgag cccgtccgtc tcggccatgc gccgcagacc ctcggcggcc
     1261 tccgtcagct cccgggtgcg gttctcgacc aggacctcca gttcctcgtt gtgccgcgcc
     1321 agggcccggg aagcctcccg ggcttcctcg taggaatcct ggatgcgccg tcccagggcg
     1381 gcggagcagg cggcgaagat ggccacccag ccgatcgcca gccccgcccg gccgggtccg
     1441 ggccccgcgg cgatccccag caggtccagc acgagcagga cgccgaacag gagggtgccg
     1501 acccgggcct gacgcaccgc ggacctgccc gcccgttcgg cccggacgag ggcggccagg
     1561 gccgcggcca ggccgcccgc cgccacggcc atgtaggaca gcgaaatcca gcgcacggcc
     1621 tcccggggca acaggaaggc cccgaccagc catgcgccgc tggcggccgc tacggcccgc
     1681 gccgtccggg tatccgactc ctccgggaag agggtgcgga ggaaatagac gaaggccggg
     1741 aggatgagga aatccggcac cgcgtccagc cggccgtgct ccgagggaag ggacgagacg
     1801 agggaatcca ggatcccccc gatcagggcc acccggaacg ccgcgaatac ggccaacagg
     1861 gaaaagggca ggtccccggc cttccggcgg cctccggcgt acatggccag gtgcagagtg
     1921 gccacggcca gcaaaaaacc caccagcagc aggctcaagc cctgcccggg ggtgggaaga
     1981 ggatattgca tatcgatatc agtatatcga gatcccggcg ggcgtccagg gtggagcgtt
     2041 cctacccgaa gaaaatcggg cggctcgatc ccgggaggtc ccgtccggtc acagccccag
     2101 ctcgagctgg ggccggtcgc cgaagatgag ctcgggatcc atgccggccg cggagagcag
     2161 ggcatccacc gcgggccgga gctggcgctg ggcgtagtgc tcgtagtcca ggggggcggt
     2221 ctcgcggccc accggctccg cccccgcctt ggtgatgcgg tactccacca ccccgcggcg
     2281 atccgtccag cccaggatgc gggcggcccg gacatggggc ggctccacct tggtgtacgc
     2341 ggccgccgtc cggcgcaggg ccttccggta gacgagccgg tcgtccagga ggcccgcccg
     2401 caggtccgcg aggacccgcc ggacgtgggc ctcgatccgc gccggcggtg cgtcccggaa
     2461 gaccaggtcc atcgtctcca cctggagctc ccgggccagg gccgttccgt cccggcgggc
     2521 ggcctccatg cccttcacct ccgtctcccc cccctcgggg cccagccgga atcccgcgta
     2581 gcctttggcc cggcccttct cgtccccctc ctcgtcgtcc tcggtccggg accgcagggg
     2641 agggatgaag aagcgggcat acaccttctc gaacttgagc tccattcggg attccaggcc
     2701 gtactcgtcc cgcacggccg ccgcgaggtc ggcgttcagc tccggggcca ggcggcgtcc
     2761 caggccgtcc agggcccccc agagcgcccc ctcgggcagg cccgaatcca cgaaggcgga
     2821 gtccgtgtcc ccgtagagca cccggaaacc cttggccgcg aaccagtccc gggcccagtg
     2881 cagccacttc cggccgtagg aggtgacggc ccccgccagg gccgtgtccg cgtagcggca
     2941 gccctccgcg cccaggaccc cgtagaagga gttcatcagg atcttgtaga cgtaggaggc
     3001 cgtctcgtcc ccccgctcga tggcggccag gcgctccgcg aagtaccgct cgatcacggc
     3061 gggcaggatc cccggttccc ggcggaaccg ggccccgttc ggggcctcga ggtcgtccgg
     3121 ccggggctcc gcggcggcca ggcggaaggc caggggatcc acgttgaagg tccgcatcac
     3181 cgagggatag aggctccgga agtcgaacac gaggacgttc cggaacaggc ccggccgggg
     3241 ctccaggatg gtcccccccg cggccccgga gacccggcgc ccctcgacct tgggggccgc
     3301 ggcgtagccc cgggccatga gctcgtccag gtagatccgc tcgaaggcgg gaatgctggt
     3361 ccaggccagg tcgatgcccg tcccggtcag ctgggcgcgc ttgagggtca ggtcgaggag
     3421 tcccgtcttg tccaggatgg ccaggaccag ctcggagtcc gccaggcagt agcgccggaa
     3481 ggcggcgggg tccccgctcc gcaggcggga cagttcctcg atcttggccc gcccccggct
     3541 ctccacggac ttgcccgtgc ccaggacctc ccgggccacc gagtccaggg tgaggtcctc
     3601 gaagcggccg gccccccggg ccagcttgag ggcgtccacg gcctggcgcc cggggatgga
     3661 ggccgcgtat cggcgccccc cgtcgagctc ccggatgtcc gcgggatcct cggtgcggcc
     3721 gatccggaag ggcaccgcga gggcctggca tctccgggac aggtgggcca ggtcgaagtc
     3781 cacgacgttc caccccgtga tcaaatccgg gtccagggcc cggatccgct cccccagggc
     3841 gcgcaggaga tccgcctccc gggagaaggg ccgcgcgagg taggtccggc cttcgtcgtc
     3901 cgcctcctcg aagggggagg ggttccgggc ggcgggatcc gcgcccgcga ggtccgggga
     3961 gaagaggccc gcggcctcgt ccaggaagag gacctcccgg agccccggcc cggccaggga
     4021 taccgccagg accgtcccgg cttccacgtc cgtctcgatg tccagggccg ccgtccggag
     4081 ggcgggaggg acctccgcgg cctccagatc cggatccagg aacacggcgt ccacgaagcg
     4141 gcccggaacg ggggtccccc ggatcacgag gcctccccgg atccggcgct ccgccaggaa
     4201 gcggtccgcg gcgcgcacgt ccgcctcgta caccggtatc cccgcggccc cgaaggcctc
     4261 ctccaggcgc cgcagttccc cccggtcccg ggcttccgtg cggtcgcagg gctcccccgc
     4321 cagggtgcgg cggccggagg acgtgaagga cgcctccagc cgggcccggg ccgctgcctc
     4381 cgaggcccgc tcgacatccg cggagcggat gaggaagtcc gggcgcaccc ggttctccac
     4441 caccgcgaag gagcgtccgt ccgaaagccg gcccgccagg agcatccgcc cccgggaatc
     4501 cgcccagccg tggaccagga accccgggtg cccggacggt cccgggtcgg gcgccgcggt
     4561 cggcccggct ccggcgtccg gtccgggagg ggacggatct accatttctc cagctgggcc
     4621 agggaggccc ggaggtcttt ggggtccggg gcctcgatcc gcaggggaag gcccgttgcg
     4681 ggatggggga tctccacgga ggcggcgtga agggccgccc gggccaggag ggggcgttcc
     4741 tcgaaggggt cgcccttcca ctttcgcttg atcgcggaca ggagcaccgg cttgccgtcc
     4801 ccgtacaggg gatcgcaggc cacgggatag cccagggcgg ccaggtggac ccggacctgg
     4861 tgggtccggc ccgtctcggg ccgcgcctcc accagggcga agtcccggaa ggccgccagg
     4921 acccggaagg ccgtgcggga gggcttgccc cggtgggcgt ccacgatggt ccggtgcagg
     4981 gcgtccccgt ccggggacag gggcaggtcg caggaggact cggcccagtc cgggaccccc
     5041 cggaccaggg cccggtagac cttggccacg gaccgggtct cgaaggcccg ggacagggag
     5101 cggtgggcct cctcgctccg ggccaggagg aggaccccgg aggtgtcctt gtccagccgg
     5161 tgcaccgccc agagccggcc ccggcccttg gacaggaggg ccgcggccac gggcaggtcc
     5221 ggatcccagc gatccggggc tgagagcacg cccgcgggct tgtccgcggc caggagggat
     5281 tcgtcttcga acaggatggg gatgttcacg gggacagtat gaagtgaacg ggagaaatgg
     5341 tgaataggca aatccggggg cgaggcgccg gaagcacccg gacggggctt cgcggcggca
     5401 ggggcccggc tcagaagagg tcccgggccc tcctgtgcac cttgtccagg gcccgcagga
     5461 actgcccccg ttcctcggag tccaggacgt cgaggggatc gcccaggacc tcggggctca
     5521 ggacgcgggc cgcctcgatc cggcccggga gctcctcgcc caggcccgtc aggcgcaggc
     5581 gatgggcgcg gcggtcgagc ttcacgcgga gccggctgag ccagccccgg gccacgcatt
     5641 tccgggccac gagggtcagg gtcggccggt ccgaagccag ctcttcggcc gcctcggagg
     5701 gccggatcgc gccgcgccgc ctcgccagtt gtatcaggag gaactgcgac agggtgatcc
     5761 cgaacccctc gagggcggcg gaagcccgtt tccgccagag gctctggacc atcgtcatga
     5821 gtcggatgcc gtccatatag ttcgtatact aatcaaataa aaaaatcacg gtcaagggcc
     5881 caaaaccctt gccgctctac ccgctttttc ggtattttct gactgacctc gtgaaccggt
     5941 tatccgaaac cgaggcgggc cctcgaggcg cacggccgag gccggcccgg aatcattcta
     6001 ggaaagggag aatccactat ggcgaaacag ctactcttca gcgaggatgc ccgcaggcgg
     6061 ctcctctccg gcgtcgagca gatctcgaag gccgtcaagg taaccctcgg tcccaagggc
     6121 cgcaacgtgc tcctggacaa gaagttcggc gcccccaccg tcaccaagga cggcgtgtcc
     6181 gtggccaagg aagtcgagct cgaggacgcc tacgagaaca tgggcgccca gctcctcaag
     6241 gaagtcgcca ccaagaccaa cgacatcgcg ggcgacggca ccaccaccgc caccgtcctg
     6301 gcctactcca tggtcaagga aggcctcaag gccgtggccg ccgggatcga ccccatgggc
     6361 ctcaagcgcg gcatcgacaa ggccgtggag ctcacggtcg aggagatcaa gaaggcctcc
     6421 aaggagatca aggagaagga cgagatctcc cacgtcgcct ccgtgtccgc caacaacgac
     6481 atggagatcg gctcccagat cgccgacgcc atggagaagg tgggcaagga cggcgtgatc
     6541 acggtcgagg agtccaagac catggacacc acgatcgagt tcgtggaggg catgcagttc
     6601 gaccgcggct acctctcccc ctacttcgtg accaaccggg acaccatgac cacggtgttc
     6661 gagaatccct acatcctgat ccacgacaag aagatctcca gcatgaagga catgctcccc
     6721 gtgctcgaga agatcgccca ggccggcaag cccctcgtga tcatcgccga ggacgtggac
     6781 ggcgaggccc tggccaccct ggtggtgaac cacctgcggg gcaccctgaa cacctgcgcc
     6841 gtcaaggccc ccggcttcgg cgaccgccgc aaggccatgc tcgaggacat cgccatcctg
     6901 accggcggcg aggtcatctc cgaggagctc ggcctcaagc tcgagagcac cgagatcggc
     6961 cagctgggca ccgccaagac cgtgaagatc gacaaggaga acaccacgat catcaacggc
     7021 gccggcaagc agaaggacat ccaggaccgg atcgcccaga tcaaggccca gatcgaggag
     7081 accaccagcg actacgaccg cgagaagctc caggagcgcc tggccaagct
//
LOCUS       DAOTEI010000181         7097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7552_length_7097_cov_4.880290, whole genome shotgun sequence.
ACCESSION   DAOTEI010000181 DAOTEI010000000
VERSION     DAOTEI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7552_length_7097_cov_4.880290"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="P5313_14180"
     CDS             <1..183
                     /locus_tag="P5313_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRY81560.1"
                     /translation="LIYFFSMATSFTKAALGAEGVGKDIELIVGNGYAKGHSDLTLNI
                     LRESAEIRALFERLYV"
     gene            complement(194..883)
                     /locus_tag="P5313_14185"
     CDS             complement(194..883)
                     /locus_tag="P5313_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81561.1"
                     /translation="MEDFRKSYGSLYAAASAATLIMLVIIPIQILVFALTPMPVPPEA
                     WFELFAANPLLGFFHADFFILVDNILIAVIYLGFYHVLKERHKGILQAALLLGFIGIA
                     VYTASNKTFELYALAGEYARTGAEARLPLLAAGRTLIAEWQGTAFDVYYVLNGVALLV
                     VAARMLGDRTFGRTTALWGLASGFLMTVPSTAGTVGLVFSLLSLVPWYVFSIRFGLTF
                     LRIARGAAVIR"
     gene            978..1601
                     /locus_tag="P5313_14190"
     CDS             978..1601
                     /locus_tag="P5313_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRY81562.1"
                     /translation="MGSAPAREGPLSRTELFDRALAIADAEGLEALSMRRLAREAGVE
                     AASLYHHLPNKSALLDGMVVRMRSEMRLPDPIPEDWRELLRAIFLEYRRVLAAHPNLM
                     SLAGRRVESDPDSGLVYLIRQGFEPEDAVELWQSLLAFTVGFSVFSSAYAEGDVSDLP
                     ADLAARMSEWRDDTCRRTLDAIIRTYDAGRVRREPRSRPAGPSKPRE"
     gene            1692..1874
                     /locus_tag="P5313_14195"
     CDS             1692..1874
                     /locus_tag="P5313_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRY81563.1"
                     /translation="MAGKFEWFKDKKDKYRFRLKAGNGQIIATSEAYETKDSCMNGIE
                     SVKKNAPDAPITEVKD"
     gene            complement(1948..2181)
                     /locus_tag="P5313_14200"
     CDS             complement(1948..2181)
                     /locus_tag="P5313_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81564.1"
                     /translation="MRIRVYAPPHADPSVLDERGFIDLPAGSRLRDLHRALKIPILAR
                     PLLLCAVNYRISGPGLALKDGDTVSYFSMAGGG"
     gene            complement(2178..2390)
                     /locus_tag="P5313_14205"
     CDS             complement(2178..2390)
                     /locus_tag="P5313_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY81565.1"
                     /translation="MAKQYPNYDYVPCMACGICVQACPFSCLELRRTGLDTYRKAYPD
                     LARREDCTGCGICASSCPVDAIRMGE"
     gene            complement(2383..4389)
                     /locus_tag="P5313_14210"
     CDS             complement(2383..4389)
                     /locus_tag="P5313_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRY81566.1"
                     /translation="MKGYLGKVLLIDLSKGTWDSQDIPETVYEGLLSGVGLGAYYLYR
                     HIPVGADPLGPDNILGFTSGLLTGTGSFMTGRWMAVCKSPLTGGWGDSNCGGTLAPAI
                     KQCGYDAIFFRGISPESVYVYVDNKGPQIRDAAAYWGMDAVEAEKALIRDHLKAEGGS
                     KKKPEVAVIGPGGEKLSRIAGISNDLGRYAARSGVGAVMGSKRLKALVLAGTRAVRPS
                     DPEGMKRLSGVFASTVRKANLPGLFTGAVFPIMGGLMGGLPSAVPLDGMLSAMMFKKF
                     GSIMNNTYGMTNGDSPVKNWDGSVKDFPAYRALNPDRIRRREVRKYHCYACILGCGGV
                     CDIRDLSGGNGEHTHKPEYETCMAFGGLVLNKDLDSIFRINDTLNRAGLDTISVGGTV
                     AFAVECFENGILTPADTGGLELRWGNARAIEALVEKICAREGIGDLLADGAKLAAERI
                     GKGSERFAVHAGGQELPMHDPRVDPMMGVTYSADPTPGRHTTSGNLYYSQSSLWDYVS
                     WAPKVRIHPKSQDFEPTETEALRNVAMTAFKMLVDGTGSCYYAMLMGVQHFPIFEYLN
                     AATGWNRTPDEYMEAGKRIQTLRQAFNAKHGVDVRAFRMHDRASGIPPASEGHNRGVT
                     LRIDEMIRQYRRAWGWDVETGLPTRETFESLGLSALPGLEYTHG"
     gene            complement(4475..4885)
                     /locus_tag="P5313_14215"
     CDS             complement(4475..4885)
                     /locus_tag="P5313_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRY81567.1"
                     /translation="MNDSRPVFSRWARLKRRAARLKRQVWAIFLAWKDPATPFPAKAV
                     IAVTIAYAVSPIDLIPDFIPFLGQLDDLIIVPALIVLALRMIPPEVAARCRRDAWRHL
                     AKGERVRTPAGTLAAVVFGIVWILLAVWLVGIFL"
     gene            4998..5204
                     /locus_tag="P5313_14220"
     CDS             4998..5204
                     /locus_tag="P5313_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81568.1"
                     /translation="MADNVDFLVKGIPNALLAMFRGLCSINGKTEGEGVIDLIIEHID
                     KNFSGDKANFKKVVEEYRAGKKKK"
     gene            5321..6010
                     /locus_tag="P5313_14225"
     CDS             5321..6010
                     /locus_tag="P5313_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY81569.1"
                     /translation="MKGISVLLVEDEPGICLTLGDRLAAEGYGVTVRNDGVSGEAAAR
                     AGGFDILLLDLNLPGRDGLSLCRNLRRDGFAAPILMLTARDGDADIVAGLREGADDYL
                     PKPFDPSVLLARMEALLRRPRSLIWDGRDPVIRFGPFSLDRERGELLREGEPVELNAR
                     EYGLLEYLALRPGKVLPRRELLERVWGRGDGDRTRTVDMHVAKLRSRLGEAEKPRHIV
                     TVHGRGYRFET"
     gene            complement(6013..>7097)
                     /locus_tag="P5313_14230"
     CDS             complement(6013..>7097)
                     /locus_tag="P5313_14230"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRY81570.1"
                     /translation="GMFTANTMNCMMEGLGLGLPGNGTIPAVYAERDRLAKDAGVAIM
                     KLVEQGTRPRDILTRKAFENAVALDLALGGSTNTALHLPAIAWAAGLELPVEIFNEIG
                     ARVPHLCSLSPAGPYHIQDLYRAGGVQAVMRRLLDAGLVDGSCRTVTGMSQAENLAGA
                     RVRDEEVIRPLDRPYHAQGGLACLKGNLAPDGSIVKQAAVLPEMMVHSGPARVFDNES
                     DAFAAIKARKIKDGDVVVIRYEGPRGGPGMREMLSPTSTLAGMGLDRTVALITDGRFS
                     GASRGAAIGHVSPEAAAGGPIALVKEGDIISIDIPAKKLELKVDAAELERRKAAWKPY
                     VQTVDSLFLNRYRKVVTSGVRGAVLD"
BASE COUNT         1223 a         2337 c         2414 g         1123 t
ORIGIN      
        1 ctcatctact tcttctccat ggccacgtcc ttcaccaagg cggccctggg cgccgagggt
       61 gtcggcaagg acatcgagct gatcgtcggc aacggctacg ccaagggcca ctcggacctg
      121 accctgaaca tcctgcggga gtccgcggag atccgtgccc tgttcgagag gctgtacgtg
      181 tagatgaccg gactcaacgg atcaccgccg ctccccgggc gatccgcagg aacgtaaggc
      241 cgaagcggat cgagaagacg taccagggta cgagggacag cagggagaac accaggccga
      301 ccgtcccggc ggtcgagggg acggtcatga ggaagcccga ggcgagcccc catagggccg
      361 tggtccggcc gaaggtccgg tcccccagca tccgggcggc caccacgagc agggccaccc
      421 cgttcaggac atagtagacg tcgaaagccg tgccctgcca ttccgcgatc agggtccgtc
      481 ccgcggccag aaggggcagg cgggcttcgg ccccggtccg tgcgtactcg cccgccaggg
      541 cgtacagctc gaaggtcttg ttggacgccg tgtagaccgc gatcccgatg aagccgagca
      601 gaagcgccgc ctggaggatg cctttgtgcc gctccttgag gacatggtag aaccccaggt
      661 agatcaccgc gatcaggatg ttgtcgacca ggatgaagaa atcggcgtgg aaaaaaccca
      721 gcaggggatt cgcggcgaac agctcgaacc atgcttccgg gggcaccggc atgggggtca
      781 gggcgaagac caggatctgg atcgggatga tgacgagcat gatcagggtc gccgcggacg
      841 cggcggcgta gagggaaccg tagctcttgc ggaaatcttc catccggtat accttctgta
      901 tgggattccc taacatcgtt agggtaaccg aagaatactg cctaacatcg ttagggtcaa
      961 gcccccaagg aggctccatg ggaagcgcgc cggctcggga gggacctctt tccaggacgg
     1021 agctgttcga ccgggcgctg gcgatcgccg acgcggaagg gctggaggcc ctgagcatgc
     1081 gcaggctcgc ccgggaagcg ggggtggagg cggcctcgct ctaccaccac ctgccgaaca
     1141 agtccgccct cctggacggc atggtggtgc gcatgcggtc ggagatgcgc ctgcccgacc
     1201 cgatcccgga ggactggagg gagcttctcc gggcgatatt cctggagtac cggcgggtcc
     1261 tggccgccca cccgaacctc atgtccctgg cgggacgccg ggtcgagtcg gatccggaca
     1321 gcggcctagt ctacctcatc cgtcagggct tcgagccgga ggacgcggtg gagctgtggc
     1381 agtccctcct ggccttcacc gtgggatttt ccgtcttcag ctccgcctac gcggaaggcg
     1441 acgtaagcga tctgcctgcg gacctggccg cccggatgtc cgagtggagg gacgacacct
     1501 gccggaggac cctggacgcg atcatccgga cctacgacgc cgggcgcgtg cgaagggaac
     1561 cgagaagtcg gcctgccggc ccctcgaaac ctcgcgagtg acacggacat ccgccgatcc
     1621 ttccttgcgc cccggcggag ggcagggtac aatcaagaag caacgtatca taccgcggca
     1681 aggaggatcc catggccggc aagttcgaat ggttcaagga caagaaggac aagtaccggt
     1741 tccggctcaa ggcggggaac ggtcagatca tcgccaccag cgaggcctac gagaccaagg
     1801 attcctgcat gaacggtatc gagagcgtca agaagaacgc cccggacgct ccgatcaccg
     1861 aggtcaagga ctagccataa tctccgccgg cgattcgcgc ccggcggaga tgtacgtcgc
     1921 ctgggccgcg tttcgacgcg gcctctccta ccctcccccg gccatcgaga aataactcac
     1981 cgtatccccg tccttcaagg ctaggccggg accggagatc ctgtagttca cggcgcacag
     2041 gagcaggggg cgtgcgagga tcgggatctt caaggcccga tgcaggtccc gcagccggga
     2101 gccggcgggc aggtcgatga agccccgctc gtccaggacc gaagggtcgg cgtgcggcgg
     2161 ggcgtagacc cggatcctca ttcccccatc cggatcgcat ccacggggca ggaggaggcg
     2221 cagatgccgc agcccgtgca atcctcccgc ctcgcgaggt ccgggtaggc cttccgatag
     2281 gtatccaggc ccgtcctgcg cagctccagg caggagaagg ggcaggcctg cacgcagatc
     2341 ccgcaggcca tgcagggaac gtagtcgtag ttcgggtatt gcttagccat gggtgtactc
     2401 cagccccggc agggcggaaa ggccgaggga ttcgaaggtt tcccgggtcg gcaagcccgt
     2461 ctccacgtcc cagccccagg cccggcgata ctgccggatc atctcgtcga tccgcagggt
     2521 gactccccgg ttgtgcccct ccgatgcggg ggggatgccc gaagcccggt cgtgcatgcg
     2581 gaaggcccgg acgtccaccc cgtgtttggc gttgaaggcc tggcggaggg tctggattcg
     2641 cttgcccgct tccatatact cgtccggcgt ccggttccag cccgtggcgg cgttcaggta
     2701 ctcgaagatc gggaagtgct ggacccccat gagcatggcg tagtagcagg aacccgtccc
     2761 gtccaccagc atcttgaagg ccgtcatggc cacgttccgc agggcttccg tctccgtggg
     2821 ctcgaagtcc tgggacttgg ggtggatccg caccttcggg gcccaggaca cgtagtccca
     2881 gagggaggac tgggagtagt acaggttccc gctggtggtg tggcggcccg gggtgggatc
     2941 cgcgctgtag gtcaccccca tcatcgggtc cacccgggga tcgtgcatgg ggagttcctg
     3001 gccgcctgcg tgcacggcga agcgctccga gcccttgccg atccgctcgg ccgcgagctt
     3061 cgccccgtcc gccaaaagat ccccgatccc ttcccgggcg cagattttct ccaccagggc
     3121 ctcgatcgcc cgggcgttcc cccagcggag ctccaggcct cccgtgtccg cgggggtcag
     3181 gatcccgttc tcgaagcact ccaccgcgaa ggcgaccgta cccccgacgg agatggtgtc
     3241 caatccggcg cggttcaagg tgtcgttgat ccggaagatc gagtccaggt ccttgttcag
     3301 gaccaggcct ccgaaggcca tgcaggtctc atactccggc ttgtgggtgt gctccccgtt
     3361 ccctccggag aggtcccgga tgtcgcagac cccgccgcag cccaggatgc aggcgtagca
     3421 gtggtacttc cggacctccc gcctacggat gcggtccggg ttcaaggcgc ggtacgcggg
     3481 gaagtccttg accgaaccgt cccagttctt gaccggggag tccccgttgg tcatgccgta
     3541 ggtgttgttc atgatggagc cgaacttctt gaacatcatg gccgagagca tcccgtccag
     3601 gggcaccgcg ctgggaagcc ctcccatgag gcctcccatg atcgggaaga cggcacccgt
     3661 gaaaagcccg ggcaggttgg ccttccgtac cgtggaggcg aagacgccgg agaggcgctt
     3721 catcccctcg ggatccgagg gacggaccgc ccgggtcccc gccaggacca gggccttgag
     3781 gcgcttggat cccatgaccg ccccgacccc ggaccgggcc gcgtaccgcc ccaggtcgtt
     3841 ggagatcccg gcgatccggg aaagcttttc cccgcccggg ccgatcaccg ccacctcggg
     3901 cttcttcttg ctcccgccct ccgccttcag gtggtcccgg atcagggcct tctccgcctc
     3961 cacggcgtcc atgccccaat aggcggcggc gtcccggatt tggggaccct tgttgtccac
     4021 gtagacgtag accgattccg gggagatgcc ccggaagaag atcgcgtcgt agccgcactg
     4081 cttgatggcg ggagccaggg tccccccgca gttggagtcc ccccagcccc cggtgagggg
     4141 ggacttgcag accgccatcc agcggccggt catgaaggag cccgtgccgg tcaggaggcc
     4201 cgaggtgaag cccaggatgt tgtccggtcc cagagggtcc gcgccgacgg gtatgtgccg
     4261 gtacaggtag tacgccccca gcccgactcc ggacaggagg ccctcgtaca ccgtctccgg
     4321 gatgtcctgg gaatcccagg ttcccttcga cagatcgatc aggagcacct tgccgagata
     4381 ccctttcatg ccttaccccc gggtcgacgg atcctcgccg ccccacgggc gaatgatacg
     4441 cgatcccgaa ggccggcgca atccgggcaa ggttctaaag aaagatgcct acaagccaga
     4501 cagccagaag tatccagacg attccgaaga ccacggcggc cagagtgccc gcgggcgtcc
     4561 ggacccgctc tcccttcgcc aaatgccgcc aggcatccct gcggcagcgg gcggcgacct
     4621 cggggggaat catccgcagg gccaggacga tcagggccgg gacgatgatg aggtcgtcca
     4681 actggcccag gaaggggatg aagtccggga tgaggtcgat cgggctgacg gcgtaggcga
     4741 tggtgaccgc gatgacggcc ttggccggga agggggtggc gggatccttc caggccagga
     4801 agatcgccca gacctggcgc ttcaaccggg cggcgcgccg cttcaggcgg gcccagcggg
     4861 aaaagacggg gcggctgtcg ttcacggcat cggtctccaa ggttcaagca cgcgggatcc
     4921 tatcacggcg caccggggaa ggcaacatcg ggtcccgggg cgcgctatac tctaaggata
     4981 tacattagga ggccgaaatg gcggacaatg tcgatttcct ggtcaagggc atcccgaacg
     5041 cactgctcgc gatgttccgg ggcttgtgct ccatcaacgg caagaccgag ggcgaggggg
     5101 tcatcgacct catcatcgag cacatcgaca agaacttctc cggggacaag gccaacttca
     5161 agaaggtcgt ggaggagtac cgggccggga agaagaaaaa atagccgtcg ggattccggc
     5221 cgatccggct ttccctggat tcccgttcct ccatccgtta tcatcgatcc ggagggactt
     5281 ccgcggaccg cgagcgcggt agtccgcggc gggaggcgac atgaagggga tatcggtcct
     5341 cctggtcgag gacgaacccg gaatctgcct gaccctggga gaccgcctgg ccgcggaggg
     5401 gtacggggtc acggtgcgga acgacggcgt ctccggggag gcggcggccc gggccggagg
     5461 cttcgacatc ctcctgctgg acctgaacct ccccggccgg gacgggcttt ccctctgccg
     5521 aaacctccga agggacggat tcgcggctcc gatcctcatg ctgaccgccc gggacgggga
     5581 cgcggacatc gtcgcgggac tccgggaggg tgccgacgac taccttccca aacccttcga
     5641 cccctcggtg ctcctggccc ggatggaggc cctgctccgg aggccccggt cgctgatctg
     5701 ggacgggcgg gatccggtca tccggttcgg acccttttcc ctggaccggg agaggggtga
     5761 actcctccgg gaaggcgagc ccgtggagtt gaacgcccgg gagtacggcc tcctggagta
     5821 cctggccctc cggcccggaa aggtcctacc gcgcagggaa ctcctggagc gggtctgggg
     5881 gcgcggggac ggggaccgga cgcgcaccgt ggacatgcac gtggccaagc tccgttcccg
     5941 cctgggggag gcggaaaagc cccggcacat cgtgaccgtc cacggccggg gctaccgctt
     6001 cgaaacctga atctagtcta aaaccgcgcc ccggacgccg gaggtcacga ccttccgata
     6061 ccggttcagg aagaggctgt ccacggtctg gacgtagggc ttccaggccg ctttccggcg
     6121 ctccagttcc gcggcgtcca ccttcagttc cagcttcttg gcggggatat cgatggagat
     6181 gatgtccccc tccttcacca gggcgatggg cccgcccgcg gcggcttccg gggagacgtg
     6241 gccgatggcc gcgccccggg aggctccgct gaagcgtccg tccgtgatca gggccaccgt
     6301 ccggtccagt cccatgcccg ccagggtcga ggtcggggag agcatctccc gcatgccggg
     6361 accgccccgg ggaccctcgt agcggatcac caccacgtcc ccgtccttga tcttccgggc
     6421 cttgatggcc gcgaaggcgt ccgactcgtt gtcgaagacc cgggccgggc cggagtggac
     6481 catcatctcc ggaaggaccg ccgcctgctt gacgatggac ccgtccgggg ccaggttgcc
     6541 cttcaggcag gccaggccgc cctgggcgtg gtagggccgg tcgaggggcc ggatgacttc
     6601 ctcgtcccgg acccgggcgc ccgccagatt ctccgcctgg gacatgcccg tgacggtccg
     6661 gcaggatccg tcgaccaggc ccgcgtcgag caggcgcctc atgaccgcct ggactccgcc
     6721 ggccctgtac aggtcctgga tgtggtaggg acccgcgggg gacagggagc agaggtgggg
     6781 cacccgggcg ccgatctcgt tgaagatctc cacgggaagc tccaggcccg cggcccaggc
     6841 gatggccggc aggtgcaggg cggtgttcgt ggagcccccc agggccaggt ccagggccac
     6901 ggcgttctcg aaggccttcc gggtcaggat gtcccggggc cgggtcccct gctccaccag
     6961 cttcatgatc gccacgcccg cgtccttggc caggcggtcc cgctcggcat agaccgcggg
     7021 gatggtgccg ttccccggaa ggccgagtcc caggccctcc atcatgcagt tcatggtgtt
     7081 ggcggtgaac atgcccg
//
LOCUS       DAOTEI010000182         7008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7688_length_7008_cov_4.524378, whole genome shotgun sequence.
ACCESSION   DAOTEI010000182 DAOTEI010000000
VERSION     DAOTEI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7688_length_7008_cov_4.524378"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="P5313_14235"
     CDS             complement(<1..675)
                     /locus_tag="P5313_14235"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HRY81571.1"
                     /translation="MSKVSILTVALAAAFLSSCSIATLGTRATDSFLSEEDPELVGQS
                     LPLLLKASEILADSKPTDVDRAVTASSLAVMYAMGFVSADAAGIPSEEVDARMEANLR
                     AKRLLQRAFRRSSAALEQRRKGSLAALASGDTTPLEKLKAKDVPLLYWTSASALGAFS
                     LDPFDPSASKHLAAAVALLERALELDPDWNFGALQELVISLAPSLPRELGGGEEYEIV
                     RALEEKR"
     gene            749..2524
                     /locus_tag="P5313_14240"
     CDS             749..2524
                     /locus_tag="P5313_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81572.1"
                     /translation="MLREFRTLLPYLKKYRLNYLLGLVFLVTVDAAQLAIPQFVRRAV
                     DDLSLGRTGEVPRLALAIVGVAAVVAAGRFLWRYFLHGAARRIEVELRDRLFDHFMVL
                     PSSFYQKNKIGDLMARSTNDLQAIRMASSMGLVTFVDGVFMSAAILVIMTARNPRITL
                     YTIIPLVPITVLILIFGKMVGARFKRVQELYSKLSDVVQETMTGIRVVKSFVKEEVFA
                     ARFARGNDEYRDASMGLAKIFGFFFPFIAFLSGLTTLILLWAGGGAVIENRMSPGDIT
                     ALLAYLEMLIWPMIGAGFTVNMIQRGATSLKRVNEILDEKPEAADRPGTIRTVPSCRA
                     PADGTPALEFRSLTFSYPEARDPALKNLTLRVPGGTTLGILGRIGSGKSTLLKLLPRM
                     LEPPEGTVFLAGRDVLDYDLETLRKTFGFVPQDTFLFSDTVRANILFAAPDLSPELFD
                     RVTSISAIDRDVKDFPAGWDTIVGEKGLTLSGGQKQRVAIARALAADPEILVFDDALS
                     AVDTETEERILEALLARRAGRTNLIISNRVSTLRHADRIAVLDGGVLTQFGTHEELLA
                     GEGFYSEIARLQALVRDPDGRPLHG"
     gene            2517..4619
                     /locus_tag="P5313_14245"
     CDS             2517..4619
                     /locus_tag="P5313_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81573.1"
                     /translation="MADFFDSDVITKEYDPRIMRRILGYVKPYRRLAVYAVAALFLAT
                     AGELALPVLIQRTVDDALLPSWYGLGTDIRGRPEAAHLGLSGREPEALGRVWVKADRV
                     SRLSAAQREALTVRGFLDREPRYLARLEAGDAETDRVLAVLGPAAERDGPWAAVRMES
                     LRALAPGDVRVLRARDGRSVAVYLAAFLLILILVLVTTYAQIWASARIGQNVMKDLRM
                     ELYRHTTGQSLAFLSRHPVGRLVTRMTGDVETINEFFTSVVISFAKDASLMVGVLAAL
                     VILSPRLGFVVLATLPPVLAASTISRIKARDAFRRQRQWLSKVNSFLSEHLSGVSVVK
                     LFAGRLRSAREFARYDDELLKANLGEMYVFATFRPVVEFLASCSLAVLLWYGAGLHAA
                     GAVSLGTLIAFINLVRMFYNPVQDISEKYTILQSAMAGGERVFKLLDAREPIEDSGTV
                     SLPKPVRGRIEFDRVWFAYKNEEWVLKDLSFAVEPGEMVAIVGYTGAGKTTIANLLAR
                     MWDVQKGRILVDGTPIRDIPLPELRGTVQPVPQDVFLFSGTVAENIRLGSPIGDEEVE
                     KAAKVVHAHGFVSRLPGGYGADLSEGAANISTGQRQLLSFARVVAHDPAIVILDEATS
                     SVDTETEKLVQKGLTELLKGRTSVVIAHRLSTIRHAHRILVLAAGRLVEEGNHEALMA
                     RGGVYANLYRLQYEKLEA"
     gene            4621..5535
                     /locus_tag="P5313_14250"
     CDS             4621..5535
                     /locus_tag="P5313_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRY81574.1"
                     /translation="MDRRSPVLSAAFVLAGAFLAAGILTSCANLPPEPAPRGEVRIGP
                     VRTEALDPSRVRFLFEVRTSNPGPRALPGLSLDCRLEAGGIPAGLSEASGAGGAGSPG
                     PVGPFSLGPGEERVWSFEGTADLRSLPASGPDEVPWRIAAVLSAPGPDGIPGEVGSAR
                     TEGTVPRVREPLLTIRSIVLVRHELVNVLLELVLDVENPNPFPVEFSAASYRFHGEGR
                     KWASGKVDRPASVPARGSAEVRLPILLNFTETGRDLFDLVAKLQVVRFRLEGQARVTA
                     PLPFLPEFRMDFDRSGAVQVERTLPTRP"
     gene            5541..>7008
                     /locus_tag="P5313_14255"
     CDS             5541..>7008
                     /locus_tag="P5313_14255"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY81575.1"
                     /translation="MPRYRIPELKLPLDHGGPEIERRILALLRTSPENLESFRLDRKS
                     FDARDKGDIRTVYGVTVSLRKALRESSGRGWMPAPPEEPYRFSAPAEPSREEGRPRPV
                     VVGAGPAGLFCALLLAENGFRPLLLERGGDVRARAESVRRFWETGNLDPECNMQFGEG
                     GAGTFSDGKLNTGVHDRPAAMRKVLEEFVESGAPPEIAWLAKPHIGTDFLVTVVRNLR
                     AKIERLDGEVRFGARVSSLEVRDGRIRSVTVNGSERIEADRVVLAVGHSARDVFEELE
                     RVGAVLEPKPFAMGLRIEHPQEDIQRAQFGPSWKHPALPRADYKLTHQARDGRGVYSF
                     CMCPGGWVVNAASEAGGVVCNGMSDFARDARNANSALVVAVRPGDFGGSGPRAGVDFQ
                     RAWEAAAFRAGGGDYALPVQTLGDFVEGRPSSRLGTVEPCIRGRYRPADLSACLPGFV
                     RDGIREALGTFGRRIRGFDRPDAVLSGVETRTSSPVRVL"
BASE COUNT         1003 a         2457 c         2404 g         1144 t
ORIGIN      
        1 gcgcttctct tcgagagcgc gcacgatttc gtactcctcg ccgccgccca gctcccgggg
       61 cagggagggg gccagggaga tcacgagttc ctggagggcc ccgaagttcc agtccggatc
      121 cagctccagg gcgcgctcca ggagggccac cgccgcggcc aggtgcttgg aggcggaggg
      181 atcgaaggga tccagggaga aggcccccag ggcggaggcg ctggtccagt agagaagggg
      241 cacgtccttg gccttcagct tttccagggg cgtcgtatcc cccgaagcca gggccgccag
      301 gctgcccttc cggcgctgtt ccagggctgc cgaggagcgg cggaaggccc tctgcaggag
      361 gcgcttggcc cggaggttgg cctccatccg ggcgtccacc tcctcggagg ggatgcccgc
      421 ggcgtcggcg ctgacgaagc ccatggcgta catcaccgcc agcgaggagg ccgtgacggc
      481 ccggtcgacg tccgtgggtt tggaatccgc caggatctcg gaggccttga gcaggagggg
      541 caggctctgg cccacgagtt cggggtcctc ctcggagagg aaggaatccg tggcccgggt
      601 gcccagggtg gcgatcgaac aggaagagag gaaggccgcc gcgagggcga ccgtcaggat
      661 cgatactttg gacatattta tatattgtag ggaacggcct ttcttgtcaa cgcttgccat
      721 ttcctgccct cgtcgatact cttgtgccat gctccgtgaa ttccgcaccc tcctcccgta
      781 cctgaaaaag tacaggttga actacctgct cgggcttgtg ttcctggtca ccgtggacgc
      841 cgcccagctg gccatccccc agttcgtgcg ccgggcggtg gacgaccttt ccctgggccg
      901 gacgggggag gtcccgcgac tcgccctggc catcgtcggc gtggcggccg tggtggccgc
      961 ggggcgtttc ctgtggaggt acttcctgca cggggcggcg cggcgcatcg aggtggagct
     1021 ccgggaccgt ctcttcgatc acttcatggt cctgccctcg tccttctacc agaagaataa
     1081 gatcggggac ctcatggccc ggtccaccaa cgacctccag gccatccgga tggcctccag
     1141 catgggactg gtgaccttcg tggacggggt cttcatgtcc gccgccatcc tggtcatcat
     1201 gaccgcccgc aatccccgga tcaccctgta caccatcatt cccctggttc ccataacggt
     1261 cctgatcctg atcttcggca agatggttgg ggcgcggttc aagcgggtcc aggagctcta
     1321 ctccaagctg tccgacgtgg tccaggagac catgaccgga atccgggtgg tgaaatcctt
     1381 cgtcaaggag gaggtcttcg cggcccggtt cgcccggggg aacgacgagt accgggacgc
     1441 ctccatggga ctggccaaga tcttcggctt tttcttcccc ttcatcgcct tcctctcggg
     1501 cctgacgacc ctgatcctgc tctgggcggg gggcggggcg gtcatcgaga accggatgag
     1561 ccccggggac atcaccgccc tgctggccta cctcgagatg ctgatctggc ccatgatcgg
     1621 ggcggggttc acggtgaaca tgatccagcg gggagccacc tccctcaagc gggtgaacga
     1681 gatcctggac gagaagcccg aggcggcgga ccggcccggc acgatccgga ccgtgccgtc
     1741 ctgccgcgcc cccgcggacg gcactccggc cctggagttc cgttccctga ccttctccta
     1801 cccggaagcc cgggacccgg ccctgaaaaa cctgaccctc cgggttcccg ggggcacgac
     1861 cctgggcatc ctgggccgca tcgggagcgg gaagagcacc ctgctcaagc tccttccccg
     1921 gatgctggaa ccccccgagg ggaccgtgtt cctggcgggg cgggacgtcc tggactacga
     1981 cctggagacc ctgaggaaga ccttcggatt cgttccccag gacaccttcc tcttctccga
     2041 cacggtccgg gccaacatcc tcttcgccgc cccggacctg tccccggaac tgttcgaccg
     2101 ggttacctcc atcagcgcca tcgaccggga cgtgaaggac ttccccgcgg gctgggatac
     2161 gatcgtgggg gagaagggcc tgaccctctc gggaggccag aagcagcggg tggccatagc
     2221 ccgggccctg gccgcggacc cggagatcct cgttttcgac gacgccctgt ccgcggtgga
     2281 cacggagacg gaggagcgga tcctggaagc cctcctggcc cggagggcgg ggcgcacgaa
     2341 cctgatcatc tccaaccggg tgtccaccct gcgccacgcg gatcggatcg ccgtcctgga
     2401 cgggggagtc ctgacccagt tcggcaccca cgaggagctc ctggccgggg aggggttcta
     2461 ctccgagatc gcccgcctcc aggccctggt gcgggacccc gacgggaggc ctctccatgg
     2521 ctgatttctt cgactccgac gtgatcacca aggaatacga cccccggatc atgcggcgca
     2581 tcctgggcta cgtgaagccc taccgccgcc tcgcggtcta tgcggtcgcc gccctcttcc
     2641 tggccaccgc gggggagctg gccctgccgg tcctgatcca gcggaccgtg gacgacgccc
     2701 tccttccttc ctggtacggg ctcgggacgg atatccgggg ccggcccgag gcggcccacc
     2761 tggggctttc cggaagggag cccgaggccc tgggccgggt ctgggtcaag gcggaccggg
     2821 tctcccggct ctccgccgcc caacgggaag ccctgaccgt ccgggggttc ctggatcggg
     2881 aaccccgata cctggcgcgc ctcgaggccg gggacgccga gacggaccgg gtcctggccg
     2941 tcctgggacc agccgccgaa cgggacggcc cctgggctgc ggtgcggatg gaatccctcc
     3001 gggccctggc tcccggggac gtccgggtcc ttagggcccg ggacgggagg tccgtagccg
     3061 tctacctggc cgccttcctc ctcatcctga tcctcgtcct ggtgacgacc tacgcccaga
     3121 tctgggcctc cgcacggatc ggccagaacg tcatgaagga cctccggatg gagctctacc
     3181 ggcacaccac ggggcagtcc ctggccttcc tctcccggca ccccgtgggg cgcttggtga
     3241 cccggatgac cggggacgtg gagaccatca acgagttctt caccagcgtg gtgatctcct
     3301 tcgctaagga cgccagcctc atggtcgggg tcctggccgc cctggtgatc ctgagtcccc
     3361 gtctgggctt cgtggtcctg gccaccctgc ctccggtcct ggcggcctcc acgatttccc
     3421 gcatcaaggc ccgggacgcc ttccgccgcc agcgccaatg gctgtccaag gtcaactcct
     3481 tcctctccga gcacctctcc ggagtctcgg tggtcaagct tttcgcgggc cgcctccgga
     3541 gcgcccggga gttcgcccgg tacgacgacg agctcctgaa ggcgaacctg ggggaaatgt
     3601 acgttttcgc caccttccgc ccggtggtgg agttcctggc ctcctgttcc ctggccgtgc
     3661 tcctgtggta cggcgcgggc ctccacgccg cgggggcggt ctccctgggg accctcatcg
     3721 ccttcatcaa cctggtgcgg atgttctaca accccgtcca ggacatctcg gagaagtaca
     3781 ccatcctcca gagcgccatg gccggggggg agagggtctt caagctcctg gacgcccggg
     3841 agcccatcga ggactccggc accgtctccc tccccaagcc cgtccggggg cgcatcgagt
     3901 tcgaccgggt ctggttcgcc tacaagaacg aggaatgggt gctcaaggac ctttccttcg
     3961 ccgtggagcc cggggagatg gtggccatcg tcgggtacac gggagccggc aagacgacca
     4021 tcgccaacct cctggcccgg atgtgggacg tccagaaggg ccggatcctg gtggacggga
     4081 cgcccatccg ggacatcccc ctgccggagc tccgcgggac cgtccagccg gtgccccagg
     4141 acgtcttcct cttctccggc accgtggcgg agaacatccg gctgggaagc cccatcgggg
     4201 atgaagaggt tgagaaggcc gccaaggtag tccacgccca cggcttcgtg tcccgcctcc
     4261 cgggcgggta cggagcggac ctctccgagg gtgcggcgaa catctccacc ggccagcgac
     4321 agctcctctc cttcgcccgg gtggtggccc acgacccggc catcgtgatc ctggacgagg
     4381 ccaccagttc cgtggacacc gagaccgaga agctcgtgca gaagggcctc acggagctcc
     4441 tcaagggccg gacgagcgtg gtcatcgccc acaggctttc caccatacgc catgcccacc
     4501 ggatcctggt ccttgcggcc ggccggctcg tggaggaggg gaaccacgag gccctcatgg
     4561 cccggggagg ggtgtacgcc aacctctacc ggctccagta cgagaagctg gaagcctagg
     4621 atggatcgcc gcagccccgt cctgtccgcg gccttcgtcc tggccggagc gttcctggcc
     4681 gccgggatcc tgacgtcctg cgccaacctc cccccggagc ccgcgccccg gggggaggtc
     4741 cggatcgggc cggtgcggac cgaggccctg gatccgtccc gggtccgctt cctgttcgag
     4801 gtacgcacct ccaatcccgg cccccgggcc ctcccgggcc tgtccctgga ctgccgactg
     4861 gaggcggggg gcatacccgc ggggctttcg gaggcctccg gtgcgggggg ggccgggagc
     4921 cccggcccgg tcgggccttt ttccctgggc cccggggagg agcgggtctg gagcttcgag
     4981 gggaccgcgg acctgagatc cctcccggcg tcggggccgg acgaggtgcc ctggcggatc
     5041 gcggccgtcc tttccgcccc cggacccgac gggatccccg gggaagtcgg atccgcccgg
     5101 acggagggga ccgtcccccg ggtccgggag cccctgctca cgatacggtc catcgtcctg
     5161 gtgcggcacg agctcgtgaa cgtcctgctg gaacttgtcc tggacgtgga gaatcccaat
     5221 cccttcccgg tggagttctc cgccgcctcc taccgcttcc acggggaggg ccgcaagtgg
     5281 gcctccggaa aggtggaccg gcccgcctcc gtcccggccc ggggaagcgc ggaggtccgg
     5341 cttcccatcc tcctcaactt caccgagacc ggccgggacc tcttcgacct ggtggccaag
     5401 ctgcaggtgg tccggttccg tctggaagga caggctaggg tgaccgcacc ccttcccttc
     5461 ctcccggagt tccggatgga cttcgaccgg tccggggcgg tccaggtgga gcggaccctc
     5521 ccgacgaggc cctgagaccc gtgccgcgct accgaatccc cgaactcaag ctccccctgg
     5581 accacggcgg gccggagatc gagcgccgga tcctggccct gctccgaacc tccccggaga
     5641 atctcgagtc cttccgcctg gaccggaagt ccttcgacgc ccgggacaag ggggacatcc
     5701 ggaccgtcta cggagtgacg gtgagcctcc gcaaagccct tcgggagtcc tccggccggg
     5761 gctggatgcc ggcgcccccg gaggagccct atcgcttttc cgcccccgcg gagccctccc
     5821 gggaggaggg ccgtccccgg cccgtggtgg tcggggccgg acccgcgggg cttttctgcg
     5881 ccctcctcct ggcggagaac gggttccggc ccctcctcct ggagcggggc ggggatgtcc
     5941 gggcgcgggc cgagtcggtc cgacgtttct gggagaccgg gaacctggac cccgaatgca
     6001 acatgcagtt cggggaggga ggagcgggga ccttctcgga cggaaagctc aacaccgggg
     6061 tccacgaccg ccccgcggcc atgcggaagg tcctggagga gttcgtggag tccggggccc
     6121 ccccggagat cgcctggctg gccaagcccc acatcggaac cgatttcctc gtgacggtgg
     6181 tccggaacct tcgggccaag atcgagcggc tggacggcga agtccgtttc ggggcccggg
     6241 ttagttctct ggaggtccgg gacggccgga tccgttcggt cacggtgaac ggctccgagc
     6301 ggatcgaggc ggaccgggtg gtcctggcgg tgggccacag cgcccgggac gtcttcgagg
     6361 aactcgagcg ggtgggggcg gtcctggagc ccaagccctt cgcgatggga cttaggatcg
     6421 agcatcccca ggaggacatc cagcgcgccc agttcggccc ctcctggaag catcccgccc
     6481 tgccccgggc ggactacaag ctcacccacc aggcccggga cggccgggga gtctactcct
     6541 tctgcatgtg ccccgggggc tgggtggtca acgccgcctc cgaggccggg ggggtggtct
     6601 gcaacgggat gagcgacttt gcccgggatg cccggaacgc caacagcgcc ctggtcgtgg
     6661 cggtgcgacc cggggacttc gggggatccg gcccccgggc gggagtcgat tttcaacggg
     6721 cctgggaagc cgccgccttc cgggcgggtg gcggggacta cgccctgccg gtccagaccc
     6781 tgggggactt cgtggagggc cgtccgtcgt cccggctcgg gaccgtggag ccctgcatcc
     6841 ggggccggta ccggcccgcc gacctgtccg cctgccttcc gggttttgtt cgggacggga
     6901 tccgggaggc cctgggaacc ttcggccgcc gcatccgggg cttcgaccgg cccgacgcgg
     6961 tcctctcggg ggtggagacc cgcacctcct ccccggtccg ggtcctgc
//
LOCUS       DAOTEI010000183         6949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7753_length_6949_cov_5.172324, whole genome shotgun sequence.
ACCESSION   DAOTEI010000183 DAOTEI010000000
VERSION     DAOTEI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7753_length_6949_cov_5.172324"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="P5313_14260"
     CDS             <1..871
                     /locus_tag="P5313_14260"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRY81576.1"
                     /translation="PGRTGRNVTAVLEPAGPAKRQLIVSGHHDSARVFNFYIDKPEKY
                     SLRVYGGIGSFAVLWIVSLAVAVLAPGPWVRAAVTISFCLDFLLVAPLWNFAGSEGTP
                     GAGDNLAASAIALELLREFRTRRDAGRGLVGTRLLFVSFDAEEAGLRGARAWAKAHAA
                     EIAGLPTWHYNMDCVYKAADARFLTSDINGSVKLSVPLAEACAAAAREAGVPAAVEPI
                     AFLTGGTDAAETAKAGARATTLIAMKWSNEERSGGYHTPKDDLSAVEPAAVELALAVG
                     VRLADRLDAGDLD"
     gene            complement(940..1938)
                     /locus_tag="P5313_14265"
     CDS             complement(940..1938)
                     /locus_tag="P5313_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRY81577.1"
                     /translation="MLVPKIKKLYNIDPRSWEHPADKAALAALRSLKGLDELVSTILS
                     VTTEKSLRLMHLASAIKVTERQYPRIFRLTAEVTDTFDWPYTPTVFVKQSVEYNAGVL
                     GAKEPFVVLNSYLLRNFDETEVKAILAHELGHIMSGHAPYKTLIWMLARISPYLLGLP
                     KIVLIPIFAALYEWDRKSELTADRAEVLALQDDGPSLNALMRMAGGEDLSEVDINEFF
                     LQAREFDEQKSLLDSVHKLLNQLWQSHPFPVERIQELRTWSASGAFKAILDGNYLKRG
                     FSAETPEEDIKAGFDFYKRDMEDSEDPLTRLASNLGKGIEKAAGNLEDAFKELFKP"
     gene            complement(2016..2363)
                     /locus_tag="P5313_14270"
     CDS             complement(2016..2363)
                     /locus_tag="P5313_14270"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HRY81578.1"
                     /translation="MDGTGPAGTGPVTGRGLGACAGGRGAGWGYGPGLGRGICRGFEP
                     GRGPGFGSGFGRGSGRRMGWFSVGYGQAGGGPATNVKSVLEERRGFLRAELERTEDLL
                     KKAPAAPGEDKDA"
     gene            complement(2518..2964)
                     /locus_tag="P5313_14275"
     CDS             complement(2518..2964)
                     /locus_tag="P5313_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HRY81579.1"
                     /translation="MEGKTRIGIIICDRYRSCAGGKCFRSLRNREGAFSRYKDREAEL
                     VGYTSCGGCPGGNIEYAGEEMVKNGATVIHLATGLVVGYPPCPNIDYFAKFLRERYKV
                     EVVVGTHPIPTKYLDMHTKLGTWNAPEWKPRIQPTLSDLATRDAYN"
     gene            complement(3082..4617)
                     /locus_tag="P5313_14280"
     CDS             complement(3082..4617)
                     /locus_tag="P5313_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY81580.1"
                     /translation="MAQEPWLAPEGTMDIVLESLRQLIDYELAVILSFEGKDTLRVRT
                     MKGPLATRRLADFSISLLERRDLALLLAAGEPRLLSTERGFVDTYAEILDLPEGHSCL
                     AAPLTVEGTAIGLLTFDHRSCGVFSDQILRFIGVISRLIAVALAQSEASRNLRDRNRL
                     LLEERNRLLARDAEAFGDLAGTSPVWVEALDAIKLVAATESPVLLLGETGTGKEEAAR
                     AIHKLSARAAGPFVAVNCSALPASLAESELFGHEKGSFTGAQALRKGRFELAHGGTLF
                     LDEIGDLPLEIQPKILRALQEGRFERVGGESPVSVDVRIVAATHVDLGAAVEAGTFRE
                     DLFYRVAVFPIRMPPLRERGQDSILIAELFAARLRSRPGWQDLAFSAEALDLIARRDW
                     PGNVRELRNAVERAAILAKGGIIGAQELCAGDWAQGRAVTPPGLPVPGVCSDSRSFPE
                     APFRVPTLREAEREHIRTALARVRGRIYGPDGAAAALGLPPSTLQSRLKKLGLDPKDY
                     RRE"
     gene            4885..5604
                     /locus_tag="P5313_14285"
     CDS             4885..5604
                     /locus_tag="P5313_14285"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRY81581.1"
                     /translation="METSDQTAIQPMPRIGDKAPAFKAVTTQGEIDFPKQYEGSWVIL
                     FSHPADFTPVCTSEFMTFASLEQKFAELNCKLVGLSVDGLYSHIAWLRTIKEKIQYKG
                     MKDVEVKFPLIEDITMEVARKYGMIQPGEAATKAVRAVFFIDPKGVIRAVIYYPLSIG
                     RNFDELIRVVQALKTADAFSVACPADWRPGDDVIVPPAGSCGVAKDRMDGKESGVTCH
                     DWFFCTKKIAKENVLAAITKK"
     gene            5760..>6949
                     /locus_tag="P5313_14290"
     CDS             5760..>6949
                     /locus_tag="P5313_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106502.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRY81582.1"
                     /translation="MGGGGTTNQDWWPEALNLDLLNQHSSRSDPMGPGFDYRKEFLSL
                     DYEGLKRDLRELMTNSQDWWPADFGHYGPFFIRMAWHSAGTYRSGDGRGGGGRGQQRF
                     APLNSWPDNANLDKARRLLWPIKKKYGRRISWADLLILAGNTALESMGFKTFGFGGGR
                     PDVWEPDKDVYWGSEKAWLAGDKRHAEAGKLENPLAAVQMGLIYVNPEGPGGNPDPLL
                     AAKDIRETFARMGMDDEETVALIAGGHTFGKTHGAGHPKHVGPEPEAAPIEAQGFGWK
                     STFGTGKGNDTITGGPEVTWTPTPTKWTPNFFKVLFETEWELTTSPAGAKQWKPKDGS
                     GPLVPDAHEAGKRHPAGMLTTDLSLRFDPVYEKISRRFMENPDAFADAFARAWFKLTH
                     RDMGP"
BASE COUNT         1176 a         2324 c         2309 g         1140 t
ORIGIN      
        1 cccgggccgc acggggcgga acgtgaccgc ggtgctggag cccgccgggc ccgcgaagcg
       61 ccagctcatc gtgagcggcc accacgactc cgcccgggtc ttcaactttt acatcgacaa
      121 gcccgagaag tactccctgc gcgtatacgg cggcatcggc tccttcgcgg tgctctggat
      181 cgtctccctg gccgtcgcgg tcctggcacc gggaccctgg gtgagggcgg cggtgacgat
      241 atccttctgc ctcgacttcc tcctggtggc tcccctgtgg aacttcgcgg gctccgaggg
      301 cactcccggg gcgggggaca acctggcggc ctccgcgatc gccctggagc tcctgcggga
      361 gttccggacc cgccgggacg cgggccgggg cctggtcggt acccgccttc tgttcgtcag
      421 cttcgacgcc gaggaggcgg ggctccgggg ggcccgggcc tgggccaaag cccatgccgc
      481 cgagatcgcg gggcttccga cctggcacta caacatggac tgcgtctaca aggccgcgga
      541 cgcccgcttc ctgaccagcg acatcaacgg ctccgtgaag ctatcggttc ctctggcgga
      601 agcctgcgcg gccgctgccc gggaggctgg ggtgccggcc gcggtggagc ccatcgcctt
      661 cctgacgggt gggacggacg ccgcggagac cgccaaggcg ggggcccggg ccaccaccct
      721 gatcgccatg aagtggtcca acgaggagcg ctccgggggc taccacaccc ccaaggacga
      781 cctgtccgcg gtggaacccg ccgccgttga gttggccctg gcggtgggcg tccgcctggc
      841 ggatcgcctg gacgcggggg acttggacta ggcggaccgg ttccggcgcg ggcagcgggc
      901 cggcgccgac tcgccgcggc cacgccgtcc gaagtcgggc tacggtttga acagctcctt
      961 gaaggcgtcc tccaggttgc ccgcggcctt ctcgatcccc ttgcccaggt tcgaggcaag
     1021 gcgggtcagg ggatcctcgg aatcctccat gtcccgcttg tagaagtcga accccgcctt
     1081 gatgtcctcc tcgggggtct cggcggagaa tccccgcttc aggtagttgc cgtccaggat
     1141 ggccttgaag gccccggagg cggaccaggt gcgcagctcc tggatccgct ccaccgggaa
     1201 ggggtgggat tgccagagct ggttcagcag cttgtgtacc gagtccagga gggatttctg
     1261 ctcgtcgaac tcccgggcct gcaggaagaa ctcgttgatg tccacctcgg acaggtcctc
     1321 cccgcccgcc atgcgcatca aggcgttcag ggaagggccg tcgtcctgga gggccaggac
     1381 ctccgcccgg tccgcggtca gctcgctctt gcggtcccat tcgtacaggg ccgcgaagat
     1441 cgggatgagc acgatcttgg gcaggccgag caggtagggg gagatgcggg ccagcatcca
     1501 gatgagggtc ttgtacgggg cgtggccgct catgatgtgg cccagctcgt gggccaggat
     1561 ggccttgacc tcggtctcgt cgaagtttcg aaggaggtag gagttcagga cgacgaaggg
     1621 ttccttggcc cccaggactc ccgcgttgta ctccacgctc tgcttgacga acacggtcgg
     1681 cgtgtacggc cagtcgaagg tgtccgtcac ctcggccgtc aggcggaaga tccgggggta
     1741 ctggcgctcg gtgaccttga tcgcggaggc caggtgcatg aggcgcaggg atttctccgt
     1801 cgtgacggac aggatcgtgg agacgagttc gtccagaccc ttcagggacc gcagggccgc
     1861 cagggccgcc ttatccgcgg gatgctccca ggaccgggga tcgatgttgt agagtttctt
     1921 gatcttcgga accagcatga tgcctcccgg cgcggcctcg cggccccgcg cgccgggccc
     1981 attgtccgat gttttggacg gttccgcaag gagtatcacg catccttgtc ttccccagga
     2041 gcggccgggg cctttttcag gaggtcttcg gtcctctcga gttccgcccg gagaaagccg
     2101 cgtcgttctt ccagcacgct cttgacgtta gtcgccggcc ccccgccggc ctgtccgtac
     2161 cctacggaga accatcccat ccttcgaccc gaccccctgc cgaatcccga tccaaaaccc
     2221 ggaccccggc ccggttcgaa tccacggcag attcctctgc ccaaacccgg gccgtatccc
     2281 catcccgctc cgcggccgcc cgcgcaggcc ccgaggcccc tgcccgtcac cggaccggtg
     2341 ccggccggtc ccgttccatc catacaaggc atatccgcct cctcgtgatt ggtggagtat
     2401 ccgcgcctac ggcgcggaga aaaaggcaaa gctcaaaatc tttctaggga tattataggc
     2461 atatgcttat aatacgtcaa gcctaccaga gcacggagac cgcggatcgc cgaatgctca
     2521 gttgtacgcg tcccgggtcg cgaggtccga gagggtgggc tggatccggg gtttccattc
     2581 cggggcgttc caggtgccca gcttggtgtg catgtccagg tacttggtgg gtatcgggtg
     2641 ggtgcctacg acgacctcga ccttgtaccg ttcccggagg aacttggcga aataatcgat
     2701 gttcgggcag gggggatagc cgacgaccag gccggtggcc aggtggatga ccgtggcccc
     2761 gttcttgacc atctcctcgc cggcgtactc gatgttgccg ccgggacagc caccgcagct
     2821 cgtgtatccg accagttcgg cctcccggtc cttgtagcgg gagaaggctc cctcccggtt
     2881 cctcagggag cgaaagcact tgccccccgc gcagctgcgg taccggtcgc agatgatgat
     2941 accgatcctg gtcttgcctt ccatatcgcc cccctctcga atttccagga taaacctgaa
     3001 cagggttact gtcaaatgcc cgattccaga gcgtcgatca tccggtccgc cccgggccgg
     3061 cgtccccgga aggggatatt cctattcccg gcggtaatcc ttcggatcca agccaagctt
     3121 cttcagtcgg ctctggaggg tgctgggggg caagcccagg gcggccgccg ccccgtcggg
     3181 gccgtagatc ctgccccgga cgcgggctag agccgtccgg atgtgttccc gttccgcctc
     3241 ccggagcgtc gggacccgga agggcgcttc cgggaaagac cgtgaatccg agcagacccc
     3301 ggggacggga agccccggcg gtgtgacggc cctgccctgg gcccagtcgc cggcgcagag
     3361 ttcctgggct ccgatgatcc cgcccttggc caggatggcc gccctttcca cggcgttccg
     3421 caattcccgg acgttccccg gccagtccct gcgggcgatc aggtccaggg cctccgcgct
     3481 gaaggccagg tcctgccagc ccgggcggct ccggaggcgc gccgcgaaga gttcggcaat
     3541 caggatcgag tcctgtcctc tctcccggag ggggggcatg cggatgggga agaccgctac
     3601 ccggtagaag aggtcctccc ggaaggtgcc cgcttccacg gccgccccca ggtccacgtg
     3661 ggtggctgcc acgatccgga cgtccacgga caccggggac tctcccccga cccgttcgaa
     3721 gcggccctcc tggagggctc gcagtatctt gggttggatc tccaggggaa ggtccccgat
     3781 ctcgtccagg aagagggtgc ccccgtgggc cagttcgaag cggcccttcc gcagggcctg
     3841 ggctcccgtg aaggaaccct tttcgtggcc gaagagctcg ctttccgcca gggaggccgg
     3901 gagggccgag cagttcaccg ccacgaaggg tcccgcggcc cgggcggaaa gcttgtggat
     3961 ggcccgggcg gcctcttcct tgcccgttcc cgtctccccc aggaggagga ccggagactc
     4021 cgtggccgcc accagcttga tcgcgtccag ggcctccacc cagaccgggg aggtccccgc
     4081 aaggtccccg aaggcctcgg catcccgggc aaggaggcgg ttgcgctcct ccaggagcag
     4141 gcggttccgg tcccggaggt tccgggaagc ctcggactgg gccagggcca cggcgatgag
     4201 tcgggagatc actccgatga accgaaggat ctgatctgaa aagacgccgc aggagcggtg
     4261 gtcgaaagtc aggaggccta tggcggtccc ctccacggtc aggggggcgg ccaggcagga
     4321 atgcccctcc gggaggtcca ggatctccgc gtaggtgtcc acgaagcccc gttccgtgga
     4381 aaggaggcgt ggttccccgg cggccaggag gagggccagg tcccggcgtt ccaggaggga
     4441 gatggagaag tccgccaggc gtcgggtggc cagggggccc ttcatggtcc ggacccggag
     4501 ggtgtccttg ccctcgaagc tcaggatgac agccagctcg tagtcgatca gctgacgcag
     4561 ggattccagg acgatgtcca tggtcccttc cggggccagc cagggctcct gggccatgcc
     4621 gagcccggcg actcgggtcc cgttgagcat cgatttttcg gtgttcaccg acatatgggt
     4681 gaaagtatcg ccgattttcc ggtggaaggc aagctttccc ggctcaaaac ccggtggaaa
     4741 cccggttgaa ccggggcggc gcgccgaact ctgtcttggc atggatcctg ctaaagagct
     4801 catggagtcc gcccagacct gtacgcggcg gaagtctcca aaaaatcatc ctcggcacga
     4861 tgtgcctatc gacaaggagt acctatggaa accagcgacc agaccgctat ccagcccatg
     4921 ccccgcatcg gggacaaggc gccggccttc aaagccgtca ccacccaggg ggagatcgac
     4981 ttccccaagc agtacgaagg cagctgggtg atcctcttca gccaccccgc ggacttcacc
     5041 cccgtatgca cctcggagtt catgaccttc gccagcctgg aacagaagtt tgcggagctc
     5101 aactgcaagc tggtggggct ttcagtggac ggcctttaca gccacatcgc ctggctccgg
     5161 accatcaagg agaagatcca gtacaagggc atgaaggacg tggaagtgaa gttccccctg
     5221 atcgaggaca tcaccatgga ggtggccagg aagtacggga tgatacagcc cggagaagcc
     5281 gccaccaagg ccgtgcgggc cgtcttcttc atagatccca agggcgtgat ccgggcggtc
     5341 atctactatc ccctgtccat agggcgcaac ttcgacgagc tcatccgggt agtccaggcc
     5401 ctcaagaccg cggacgcctt ctccgtggcc tgtcccgcgg actggcgccc cggggacgat
     5461 gtgatcgtgc cccccgccgg atcctgcggg gtggccaagg accgaatgga cggcaaggag
     5521 tccggggtca cctgccacga ctggttcttc tgcaccaaga agatagccaa ggagaacgtc
     5581 ctggcggcta tcaccaagaa ataggcgccc ggcacggctt caaggggccg gttccggccg
     5641 gccccgatac gcatggatac gtgtcccgcg gggagcgccg ggcccccggg accggaacgg
     5701 gaacccggcg gaaggagaga accgtgagcg acgcgaagac gggcccggcg cacgggcaca
     5761 tgggcggcgg cggaaccacc aaccaggatt ggtggcccga agcgctgaac cttgacctgc
     5821 tcaatcagca ctcgtccagg tccgacccga tggggccggg cttcgactac cggaaggaat
     5881 tcctttccct cgactacgaa ggcctcaagc gggacctccg ggaactcatg acgaattccc
     5941 aggactggtg gcccgcggac ttcggccatt acgggccctt cttcatccgt atggcctggc
     6001 acagcgcggg aacgtaccgc tccggggacg gccgcggagg cggtggccgg ggccagcagc
     6061 gtttcgcgcc cctcaacagc tggccggaca acgccaacct cgacaaggcc cgccgccttc
     6121 tctggccgat caagaagaag tacggccgca ggatttcctg ggcggacctc ctgatcctgg
     6181 cgggcaacac ggccctcgag tccatgggct tcaagacctt cgggttcggc gggggccgcc
     6241 cggacgtctg ggagcccgac aaggacgtct attggggctc cgagaaggcc tggctcgcgg
     6301 gggacaagcg ccatgccgag gccggcaagc tcgagaatcc cctggccgcg gttcagatgg
     6361 gtctcatcta cgtgaacccc gagggtccgg gcggcaatcc cgaccccctc ctggctgcca
     6421 aggacatccg ggagaccttc gcccgcatgg gcatggacga cgaggagacc gtcgccctca
     6481 tagcgggtgg ccacaccttc ggcaagaccc acggcgcggg gcatcccaag cacgtcgggc
     6541 ccgaacccga ggccgcgccc atcgaggccc agggtttcgg ctggaaaagc accttcggca
     6601 cgggcaaggg aaacgacacc atcacggggg gccccgaagt gacctggacc ccgactccca
     6661 cgaaatggac ccccaacttt ttcaaggtcc tcttcgagac cgaatgggag ctcacgacga
     6721 gccccgcggg cgccaagcag tggaagccca aggacgggtc gggtcccctg gtgccggacg
     6781 cccacgaagc cgggaaacgt catcccgcgg gcatgctgac cacggacctt tccctgcgct
     6841 tcgaccccgt ctacgaaaag atctctcgca ggttcatgga gaacccggac gccttcgccg
     6901 acgccttcgc ccgggcctgg ttcaagctga cccaccgcga catgggtcc
//
LOCUS       DAOTEI010000184         6909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7820_length_6909_cov_4.474613, whole genome shotgun sequence.
ACCESSION   DAOTEI010000184 DAOTEI010000000
VERSION     DAOTEI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7820_length_6909_cov_4.474613"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1443
                     /locus_tag="P5313_14295"
     CDS             196..1443
                     /locus_tag="P5313_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HRY81583.1"
                     /translation="MDLSEKLSILAAGARYDASCASSGSDRASSRGACTPSGICHSWT
                     GDGRCVSLIKVLYSNVCRLDCAYCPNRAGADIPRTSFTAEELIRITKEFYRRNYVEGL
                     FLSSGIFADPDIVMDRLTEVARRLRRDEGFGGYIHLKAIPGSGERALREAGRWADRLS
                     ANLELPTSDSLAALAPQKDGGEILGAMRFLSGEIREAAGPGRGTPRFAPAGQSTQIIV
                     GASPEPDRTVLRLASGLYGKLGLRRVYYSAFVPVNRDPRLPSGTPPLLREHRLYQADW
                     LLRFYGFSVDEIAGESEPNLDASLDPKTAWALRHPEAFPVDLARAGREELLRVPGIGV
                     TGAARILETRRAGGLSLEVLPRLGIAWKRARHFVAVRGSSAGIEGDPDRLRALLAAPG
                     SGGPGVRSSASSAVPRLQREFQF"
     gene            1452..2291
                     /locus_tag="P5313_14300"
     CDS             1452..2291
                     /locus_tag="P5313_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HRY81584.1"
                     /translation="MRTSERLERTLIAVANLLGRTAGGRGRGDLPPSTGTTPAGGRAG
                     GAGLRRVGRPCAPRGQLELELTDCPDGVSAGSAPSPPVSGRCGLPVEERDPGALRAER
                     ALRALGGDVWETFEYSLRTDLAVEGDAAAFAALALLCGPEAAADRSRPEVRAVRAAAF
                     KVRREVHRLLGLLRFAPDPDGVLTARCEPDNEILDLLAPAFRRRFGEDPFRIVDLRRG
                     TTVGSEPDARARPDEPASDTDPANLWRAYYKAAENPARSNPRLRLQFMPRRYWKHLPE
                     LDA"
     gene            2391..2783
                     /locus_tag="P5313_14305"
     CDS             2391..2783
                     /locus_tag="P5313_14305"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRY81585.1"
                     /translation="MRLSEATIGQDFEILAVRIQREVGRRLADMGFTEGARGRVVRGG
                     AFRGPLQVRIRDYDILLRRCEAEGIEVVPVGGGHAEEPAGRSRGFGVPGFRRRGWGPR
                     RHGGIGSRDGLPGSCGDLDGPAPRESGA"
     gene            2780..4924
                     /gene="feoB"
                     /locus_tag="P5313_14310"
     CDS             2780..4924
                     /gene="feoB"
                     /locus_tag="P5313_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRY81586.1"
                     /translation="MSALAPRVIRVALAGNPNSGKTTLFNALTGAHHKVGNYPGVTVE
                     KREGVRVRDGREFRFVDLPGTYSLTAYSLDEVVARDFILDEKPDVIVDVLDSTNLERN
                     LYLCLQFQEFGIPVVGALNMSDEAAAKGIEIDADGLGKVLDIPMVKTVGPKGIGIDEL
                     LDAVEARMESSRAEPRMPGYGEELEAGIGRISDLLASDPDFAARRPSRWLAVKLLEKD
                     DHAARIASGHPRSSEIEAAVRRSVQWVERHFGKDAEIVVSEQRYGYIRGAVKETVRVV
                     RKPDFSRTEAVDRIVMHPLLALPIFAGVLWAVFQLTFLIGEYPMGWLETLFGRLGEAA
                     ESALPEGLLRSLVVDGILGGVGGVLSFVPLIVILFFLLSLLEDVGYMSRAAFATDKLL
                     HTFGLHGQSVLPMMLGFGCSVPAILAARTLKSRRDRIATILTIPFMSCGAKLPVYVLL
                     AGAFFPDAAAGMVLLIYAIGVALSLASSRILHNTVLRGEPTPFVLELPPYRAPTLRGV
                     LWHVRDKTWQYVKKAGTVILAASVLIWALTSFPARPSAPGEAEALAARYAAEHPGADP
                     GAAEAWAQTSRATAALEYSAAGRIGRFIEPAVRPLGFDWKVGVATLTGFAAKEVVVST
                     LGILYRTGLEETEESGSLREALRADPLFSPLTAFVLMLFTLIIPPCFAALATIRAELG
                     WGWLGFVVAYFLALGWGLGFAVYQIGNLAGLG"
     gene            4921..5103
                     /locus_tag="P5313_14315"
     CDS             4921..5103
                     /locus_tag="P5313_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81587.1"
                     /translation="MSGSAELAAVVGIVGAAAVGLVLALVRAGRGRSCCSPGDAGRRT
                     RGAGSRRSGGRGLSPR"
     gene            complement(5090..5941)
                     /locus_tag="P5313_14320"
     CDS             complement(5090..5941)
                     /locus_tag="P5313_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY81588.1"
                     /translation="MKIAMYTRNGVRGIAVLSAEGPRGLEEGGPGWPGDLDTLISGGA
                     DLRDVGARLSSAPLLDAGTISYLPPLVRPSKILCIGLNYRDHSSETGLKLPDYPAVFA
                     RFPSCLVGHGQPLVKPAVSDQFDYEGELVAVIGRRVRKVSREEAPDFVAGYSAFNEAS
                     VRDFQMRTSQWTVGKNFDGTGAFGPWLVTPEELPPGARGVRIRTRLNGQTVQDGNTSD
                     MVFDVADLVSILSRTMTLEPGDVLVTGTPSGVGMARKPPLFMKPGDVCEVEIEGIGIL
                     RNPVIEG"
     gene            6074..>6909
                     /locus_tag="P5313_14325"
     CDS             6074..>6909
                     /locus_tag="P5313_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81589.1"
                     /translation="MLDRYLFADWDEPPGAMDFEETFADLLGQAAAGVEAVLGREESG
                     TVPKEAEDPFWDFCVRLYVRAPALVNVLLNYKICVEHGLPLHPTVYYDLGEARKYNLR
                     YPLGELERANNLYRDGVEVARAVVRLDPGIRELAGSLLRRLPVLVRDFVYTGIRDKYT
                     WRASEPAKIRALADRILAACKPDILVGAAHGSILPGLLLAEYLGVPLYFVRFSMFKRK
                     DDAPIVSLSDEVFLSAYRDGTALLFDEDVAKGTTLELFERRLAPLFPNRRTASVIRHS
                     GAS"
BASE COUNT          984 a         2430 c         2398 g         1097 t
ORIGIN      
        1 ggagcggacg gagagcacgg actccaatgc atacgaaccg atcatggccc cactgtacac
       61 cggcccccgg cggctgtcaa tctcggcaaa ttcgcgcggc cgatccgggg tcccgggact
      121 ctccgggaat caggggtccc ggcccctcca tcgggtctcc ttgccagaaa gactatacgg
      181 ttgtatagtc attcaatgga cctatccgaa aaactctcga tcctggcggc gggggcccgg
      241 tacgacgcct cctgcgcctc cagcggatcg gaccgcgcat cctcccgggg ggcctgcacc
      301 ccctcgggga tctgccactc ctggaccggg gacggccgct gcgtctccct gatcaaggtc
      361 ctctattcca acgtctgccg cctggactgc gcctactgcc cgaaccgggc gggcgcggac
      421 atcccccgga cctccttcac cgccgaggag ctcatccgga tcaccaagga gttctaccgc
      481 cggaactacg tcgaggggct tttcctatcc tcggggatct tcgcggatcc cgacatcgtg
      541 atggaccggc tgaccgaggt ggcccggcgg ctccggaggg acgagggctt cggggggtat
      601 atccacctga aggcgatacc cgggtccggg gagcgggccc tccgggaggc gggacgctgg
      661 gccgaccggt tgtccgccaa tctggagctc ccgacctcgg acagcctggc ggccctggct
      721 ccccagaagg acgggggcga gatcctcggg gccatgcgct tcctctccgg cgagatccgg
      781 gaggcggccg gaccgggtcg gggcaccccg aggttcgccc ccgcggggca gagcacccag
      841 atcatcgtgg gagcctcccc ggagcccgac cgcacggtcc tccgcctcgc ctccggcctg
      901 tacgggaagc tgggcctccg gcgcgtctac tactccgcct tcgtgcccgt caaccgggat
      961 ccccggctgc cgtccggaac ccctcccctg ctccgggaac accggctcta ccaggcggac
     1021 tggctcctgc ggttctacgg gttttcggtg gacgagatcg cgggggagtc ggagccgaac
     1081 ctggacgcgt ccctggaccc gaagaccgcc tgggccctgc gccaccccga ggccttcccg
     1141 gtggacctgg cccgggcggg ccgggaggag ctcctccgag tgcccggaat cggcgtgacc
     1201 ggagccgccc ggatcctgga gacccggcgg gccggggggc tctccctgga ggtcctgccc
     1261 cggctgggga tcgcgtggaa gcgggcccgg cacttcgtcg cggtccgcgg atcctccgcg
     1321 gggatcgaag gggatccgga tcggctccgg gccctccttg ccgctccggg ctccgggggc
     1381 cccggagtcc gttcctcggc ctcatcggcg gtcccccggc tgcagaggga attccaattc
     1441 tagggggacc gatgaggaca tccgagagat tggagcggac cctgatcgcg gtcgccaacc
     1501 tgctgggaag gaccgccgga gggcgcggac gcggcgacct cccgccttcg accgggacca
     1561 cccccgcggg cggacgggcc ggcggcgccg gcctccgccg cgtcggccgc ccgtgcgccc
     1621 cccggggcca gctcgaactg gagctgacgg attgccccga cggcgtctcc gcggggtccg
     1681 cgccgtcccc cccggtttcc ggccggtgcg gcctccccgt ggaggagcgg gatcccggag
     1741 ccctccgcgc ggaacgggcc ctgcgggccc tggggggcga cgtctgggaa accttcgagt
     1801 actccctccg gaccgatctg gccgtggagg gggatgcggc cgccttcgcg gccctggccc
     1861 tgctctgcgg ccccgaggcc gcggcggacc ggtcccggcc cgaggtgcgc gccgtccggg
     1921 cggccgcctt caaggtccgc cgggaggtcc atcggctcct gggcctgctc cgcttcgccc
     1981 cggacccgga cggcgtcctc acggcccgct gcgagccgga caacgagatc ctggacctcc
     2041 tggcgcccgc gttccgacga aggttcgggg aggatccctt ccgcatcgtc gacctgcggc
     2101 gcggaacgac cgtcggatcg gagccggacg cccgggcccg tccggacgaa ccggcgtcgg
     2161 acacggatcc cgcgaaccta tggcgggcct attataaggc cgcggaaaat ccagcccggt
     2221 cgaacccccg ccttcggttg cagttcatgc cccggaggta ctggaagcac ctgccggaac
     2281 ttgacgcttg acgtcaggtt gagggctatg ttaggataaa caaacaagag gcccatcggc
     2341 ctcgataata atccgctcaa tgttgttgaa cgataacttc tggagggaca atgcggttgt
     2401 cggaggcgac gatcggccag gatttcgaga tcctggccgt caggatccag cgggaagtcg
     2461 gccgcaggct tgccgacatg ggattcaccg agggcgcccg cggccgggtc gtgcggggag
     2521 gcgccttccg ggggcccctc caggtccgga tccgggacta cgacatccta ctccggcgct
     2581 gcgaagccga gggaatcgag gtagtgcccg taggcggggg acatgccgag gagcccgccg
     2641 ggcggagccg gggctttgga gtcccgggat tccgcaggcg cggatggggc ccgcgacggc
     2701 atggaggcat cggaagccgc gacggacttc caggatcctg cggtgatctc gacggtcccg
     2761 cgccgcggga gtccggagca tgagcgccct ggcaccccgg gttatcaggg tcgccctcgc
     2821 ggggaacccc aactccggca agaccaccct cttcaacgcc ctgaccgggg cccaccacaa
     2881 ggtcggcaac tatcccggcg tcacggtgga gaagcgggag ggcgtccggg tacgggacgg
     2941 ccgggaattc cggttcgtgg acctcccggg aacctacagc ctgaccgcct attccctgga
     3001 cgaggtcgtc gcccgggatt tcatcctgga cgagaagccg gacgtcatcg tggacgtcct
     3061 ggactccacg aacctcgagc ggaacctcta cctctgcctg cagttccagg agttcggcat
     3121 tcccgtggtc ggcgccttga acatgagcga cgaggccgcg gccaagggca tcgagatcga
     3181 cgcggacggc ctgggcaagg tcctggatat ccccatggtg aagaccgtgg gcccgaaggg
     3241 gatcggcatc gacgagctcc tggacgccgt cgaagcccgc atggagtcct cccgggccga
     3301 gccccggatg ccgggatacg gggaggagct cgaggccgga atcggccgga tttccgacct
     3361 gctggcctcc gatccggact tcgccgccag gcggccttcc cgctggctgg ccgtcaagct
     3421 cctggagaag gacgaccatg ccgcccggat cgcctccggg catccccgga gctcggagat
     3481 cgaggcggcc gttcgccgat ccgtgcagtg ggtggagcgg cacttcggca aggacgccga
     3541 gatcgtggtc tccgagcaga ggtacggtta catccggggt gcggtcaagg agacggtccg
     3601 ggtcgtccgc aagccggatt tctcccgcac cgaggccgtc gaccggatcg tcatgcaccc
     3661 cctcctggct ctgccgatct tcgcgggggt gctctgggcg gtcttccagc tgaccttcct
     3721 gatcggggag taccccatgg gctggctgga gaccctcttc ggccggctgg gggaagcggc
     3781 ggaatccgcc cttcccgagg ggctcctgag gtccctggtg gtggacggaa tcctgggcgg
     3841 cgtcgggggg gtcctctcct tcgtccccct gatcgtgatc ctctttttcc tgctgtccct
     3901 gctggaggac gtgggctaca tgtcccgggc ggccttcgcg acggacaagc tcctgcatac
     3961 cttcggcctc cacggccagt cggtgctccc catgatgctg ggcttcggat gttccgtccc
     4021 cgcgatcctg gcggcccgga ccctgaaaag ccgcagggac cggatcgcca cgatcctcac
     4081 catccccttc atgagctgcg gcgcgaagct accggtctac gtcctcctgg ccggagcctt
     4141 cttcccggat gccgcggccg gcatggtcct gctgatctac gcgatcggtg tggctctgtc
     4201 cctcgcctcg tctcggatcc tgcacaacac cgtcctccgg ggcgagccca cacccttcgt
     4261 gctggagctg cccccctacc gggcacccac cctgcgggga gtgctctggc atgtccggga
     4321 caagacctgg cagtacgtga agaaggccgg gaccgtgatc ctggcggctt ccgtcctgat
     4381 ctgggccctg acgagcttcc cggcccgccc gtccgcgccg ggggaggcgg aagccctggc
     4441 ggcccgatac gcggcggagc atcccggggc ggatcctggc gcggcggagg cttgggccca
     4501 gacctcccgg gccacggcgg ccctggaata cagcgcggcg ggacggattg gaaggttcat
     4561 cgaaccggcc gtccgacccc tgggcttcga ttggaaggtc ggcgtggcca ccctgacggg
     4621 tttcgcggcc aaggaggtcg tggtctcgac cctgggcatc ctgtaccgga ccggtctcga
     4681 ggagaccgag gagagcggct ccctgaggga ggccctgcgg gcggatcccc tcttctcgcc
     4741 gctcacggcc ttcgtgctca tgctcttcac cctgatcatc cccccctgct tcgcggccct
     4801 ggccacgatc cgggcggaac tgggttgggg atggctgggc ttcgtggtcg cgtacttcct
     4861 ggccctgggc tggggcctcg gcttcgcggt gtaccagatc gggaacctgg cgggcctggg
     4921 atgagcggtt ctgcggagct cgcggccgta gtcgggatcg tcggggcggc ggccgtcgga
     4981 ctcgtcctcg ccctggtccg ggcgggccgg ggacggtcct gctgctcccc cggggacgcc
     5041 gggcgccgca cccgcggggc cggctcccga aggtccggcg gccgggggct cagccctcga
     5101 taaccggatt ccggagaatc ccgatcccct cgatctccac ctcacagacg tccccgggct
     5161 tcatgaacag gggcggcttg cgggccattc cgactccgga gggcgtcccg gtgaccagca
     5221 cgtccccggg ctccagggtc atggtccggc tgaggatgga gaccaggtcc gccacgtcga
     5281 agaccatgtc cgaggtgttg ccgtcctgga cggtctgtcc gttaaggcgg gtccggatcc
     5341 ggaccccccg ggcccccggg ggcagctcct ccggggtcac gagccagggc ccgaaggccc
     5401 cggtgccgtc gaagttcttg cccacggtcc actggctggt ccgcatctgg aagtcccgga
     5461 ccgaggcctc gttgaaggcc gaatagcccg ccacgaagtc cggtgcctcc tcccgggaga
     5521 ccttccggac ccggcggccg atcacggcca cgagctcgcc ctcgtagtcg aactggtcgg
     5581 agaccgccgg cttcaccagg ggctggccgt ggccgaccag gcaggagggg aagcgggcga
     5641 acacggcggg atagtcgggg agcttgagac cggtctccga ggagtggtcc cgatagttga
     5701 ggccgatgca gaggatcttg gacggccgga cgaggggggg caggtaggag atagtcccgg
     5761 cgtccaggag gggagccgac gacagccggg ccccgacatc ccggaggtcc gccccgccgg
     5821 atatcagggt gtccaggtcc ccgggccagc ccggaccgcc ttcctcgagg cccctcgggc
     5881 cttcggcgct caataccgcg atcccccgga ccccgtttcg cgtatacatc gctatcttca
     5941 tgcgtcccct ccgccctcgg ccgccgcgtt cagcctgcct tgatgtggac cggattctac
     6001 caggaaaccg gcgcgcgcgt caggcccggc cgccttcgtt gcgggcggct ccggggccgg
     6061 atatactccc tccatgctgg accgatatct cttcgcggac tgggacgagc cgcccggggc
     6121 catggatttc gaggaaacct tcgcggacct cctcggacaa gcggcggcgg gtgtcgaggc
     6181 cgtcctgggc cgggaagagt ccgggaccgt gccgaaggaa gcggaggatc ccttctggga
     6241 cttctgcgtc cgcctctacg tgcgcgcccc cgccctggtc aacgtcctgc tcaactacaa
     6301 gatctgcgtc gagcacggct tgcccctgca tccgacggtg tactacgacc tcggggaggc
     6361 gaggaagtac aacctccgct atcccctggg ggaactggag cgggccaaca acctgtaccg
     6421 ggacggggtg gaggtggccc gtgccgtggt ccgcctggac cccggcatcc gggaactggc
     6481 cgggtccctc ctgcggaggc ttccggtcct ggtccgggac ttcgtctaca cggggatccg
     6541 ggacaagtac acctggcggg cttccgaacc cgccaagatc cgggcccttg cggacaggat
     6601 cctcgcggcg tgcaagccgg acatcctggt gggggcggcc cacggctcca tcctgcccgg
     6661 gctcctgttg gcggaatacc tgggcgttcc cctgtatttc gtgcggttca gcatgttcaa
     6721 aagaaaggac gacgccccga tcgtctccct ctcggacgag gtcttcctgt ccgcctaccg
     6781 ggacggcacg gccctgctct tcgacgagga tgtggccaag ggaaccaccc tggagctctt
     6841 cgagcgccgc ctggcccccc tcttcccgaa ccgacgcacc gcctcggtca tccggcattc
     6901 gggagcctc
//
LOCUS       DAOTEI010000185         6854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7905_length_6854_cov_5.236211, whole genome shotgun sequence.
ACCESSION   DAOTEI010000185 DAOTEI010000000
VERSION     DAOTEI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7905_length_6854_cov_5.236211"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1019
                     /locus_tag="P5313_14330"
     CDS             219..1019
                     /locus_tag="P5313_14330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HRY81590.1"
                     /translation="MRLSAFIVNAFAEDLACGNPAGVVFSEEALPERTMRDLAADLGK
                     SETAFLTPRGRDYGIRWFSPLQEMPLCGHATLAAAWVIFGRERDRTGIEFSHAGGAIS
                     ARRAEDGFIGLTFPLDGYERIEPDPEWLGLLGLDRAEDCVRGRGTGKVILAARADFDL
                     ADLRPDFRRMRTRLGVWERGVAVTRPSAAFDFETRYFNPWAGVDEDPVTGSVHTVLAG
                     YWRDRLGRTVLTGRQASQRPGTIRMRIDGEMVELAGKARIVFEGTADV"
     gene            complement(1038..1646)
                     /locus_tag="P5313_14335"
     CDS             complement(1038..1646)
                     /locus_tag="P5313_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81591.1"
                     /translation="MRTQPRPAPAARIRTILAFALAAALPVIGQPTPSAPGPAPASPA
                     SAPSSGLPRGFRGVELGMSLSEVETVLKADELFLYRGGPDVTLLPRPDEKLLEVQGLS
                     YVKRAFFQFHEDKLFSAIYVLNPERVDHYSVFTRLSDRYGKPGSLSPRESVWSDGSVR
                     LSLERPLTVKYLDLGTLEKFMQGGQARQSWEDLSRQDFLESF"
     gene            complement(1669..2187)
                     /locus_tag="P5313_14340"
     CDS             complement(1669..2187)
                     /locus_tag="P5313_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY81592.1"
                     /translation="MMRKAAKNAAALGKVGILQDLSEEQLGRILEIAEPLEVKAGTVI
                     MREDEPGDEVFLFMEGRVDVSKNLTLKIGRTGFGRAEKSMTKLNAGQASVFGEMALFG
                     TEPRSATVTAASDCLLYRIRREDFAALCDADPDLGLKVVRRIAAVLGARLRKGNEDVL
                     KLSTALSIALSK"
     gene            complement(2184..3431)
                     /locus_tag="P5313_14345"
     CDS             complement(2184..3431)
                     /locus_tag="P5313_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRY81593.1"
                     /translation="MPTRTRTDSRRPRARHGTDPEGRTLLSGLSDRWSSLKNDAARAN
                     LPRLLVFFLSAVVTGAVLVHLAESGWEDGMFARLFDGIWWSVVTIASVGYGDKYPRTD
                     VGRSFAILLILSGVVLTSLISGTIASIFVERRIREGKGLRDVKLKRHILVCGWNAKAR
                     NVLEGISGSEEAARTPVVLVCQMESDLFDDLKTQFPGLDLRFVRGDHTQETVLRRASA
                     GTARACILLPDESGAGGTANADERTIMACLAVKALNPEIHASAEILKTESEQHLVRAQ
                     ADDVIVNGEFAGRLLSAAGFEPGLPGAARRLFSGESRLRQSPIPPALVGRTFREASGW
                     YLSEKSAVLVGVLSKGKGMALEDLLSDDSSAIDAFIKRKFQEAEMDLSREAGSGNEIR
                     LAPGPDYVIRDTDWAFTVGSGAR"
     gene            3535..4419
                     /locus_tag="P5313_14350"
     CDS             3535..4419
                     /locus_tag="P5313_14350"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRY81594.1"
                     /translation="MSSFDFPTRGGDPRVVRADPDFLAVSKPAGMHTAPAPAGDPDPS
                     SPDLASWVFERYPEIRGIRGRDRGEGGLLHRLDRETSGLVLFARTEESFRALSAAAAS
                     GLFRKDYILRARPSRSGLPGSRPVRTAPGGVDPEAWAAWLDRGTPPGALGGTDCAPGA
                     AEGLRGILAGALESGRPVFVESRFRPFGPGAARVACAAADADLGRSRGDWGLETYRTD
                     ILETGRTGSVLEVRVSLTRGFRHQVRAHLAWIGLPLEGDGLYGGGEGERLALHAAALD
                     FPRPGTGERIRVEDRGVQ"
     gene            4487..6202
                     /locus_tag="P5313_14355"
     CDS             4487..6202
                     /locus_tag="P5313_14355"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HRY81595.1"
                     /translation="MSEPSTFDSLVRGLSREERRDLLTRIRGTAEVSDEPMFRPETVR
                     EPPVDYSAQYRELGPLARLVILVRRLFSGKPRDELVKELLLRHLARRIELGSPGLVSY
                     SRRVFLNRMLAELRDLRDGARYIYDLLDRSVEKDRGAFYAFLASLELEEQHQRLLSET
                     DPFLFAQTNSLAGEPEVRTAVWTAFDGILSSMPEESRRRMYADVRSLAFLKRVAGFLY
                     DRLFSCFEGAGGAAPESPFVTARDLMSELADILYSMASPPSLKLMESLLVFQMREEME
                     NPDFDAETALKTQLARVEEALARIRSFNARVPLHEILRVVMEDPNYRPRELAGGEDWF
                     AIYKGFWKERIEKQLSLYGTARRMQDLQSDIAGFLGSSEPVRFEYINEEGREGSPPVR
                     LEKALGFLSAFYERLFVPEMNRTLKILLIEGEFYKKDNRVEYTDAYNELLQIPEALSG
                     LDARLGPGGDLGSAWVQAKEELVSLPLKRRKVQAALQAANGEAEALLSRSREAMKRMQ
                     AILKGLLKGEAGGRYDSLSNLSYIEGKGNKEFLRNLDSIKNRLERTVHFLDELEKLGM
                     TREPA"
     gene            6199..>6854
                     /locus_tag="P5313_14360"
     CDS             6199..>6854
                     /locus_tag="P5313_14360"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRY81596.1"
                     /translation="MTCTELSLDSVRSGGFARFPLGADPGAPWALLSLRPLGSMRFLH
                     DRPNPLREDFFRDRGIDPGRVLAVELVHSRTVLFPKGPAELRGIRADGLIVEPGPWVP
                     TVTVADCMPIWIRHAPSGAYGILHSGWQGTGILAAAVRGLEERFGARPSEIDAFLGPA
                     IGPCCYRVPEDRARGFRAEFGPEAARLEDGAWRLDLGAANLGIARSLGLRSVTRISGC
                     T"
BASE COUNT         1032 a         2338 c         2392 g         1092 t
ORIGIN      
        1 agaccctgga gagcgggatc gccggcctct tcccctgcgg ggagggggcg ggctacgcgg
       61 gagggatcct ctcggcggcc gtggacgggg tgcgggcggc cgaagccgtt gctacccgcg
      121 tccgttacat cctgtaaccg ccgaacttcg ttcggcgatt gaccgtggcg ccgcatccgt
      181 gcggcgcgta accgccggga gcggatggga gattcgccat gcgtctgtca gctttcatcg
      241 tgaacgcctt cgccgaggac ctggcctgcg ggaaccccgc gggggtcgtg ttctcggagg
      301 aggcccttcc ggaacggacg atgcgggacc tggccgcgga cctgggcaag tccgagacgg
      361 ccttcctcac tccccggggc cgggactacg gcatccgatg gttctctccc ctccaggaga
      421 tgcccctctg cggccacgcg accctggccg cggcctgggt gatctttggc cgagagcgag
      481 accggaccgg tatcgagttc tcccacgcgg gcggggcgat ctcggcacgc agggcggagg
      541 acggcttcat cgggttgacc tttcccctgg acgggtacga gcgcatcgaa ccggaccccg
      601 agtggctcgg cctcctgggc ttggaccggg cggaggactg cgtgcgcggg cgcgggaccg
      661 ggaaggtgat cctggcagcc cgggcggact tcgacctggc cgacctccgg cccgacttcc
      721 gccggatgcg gacccgcctt ggagtctggg agcgcggtgt ggccgtgacc aggccctccg
      781 cggccttcga cttcgagacc cggtacttca acccctgggc gggggtggac gaggatcccg
      841 tcaccggctc ggtgcacacc gtactggcgg ggtactggcg cgaccgtctg ggccggaccg
      901 tccttacagg acgccaggct tcccagaggc ccgggacgat ccggatgagg atagacggag
      961 aaatggtgga gctggccggg aaggcccgga tcgtgttcga ggggacggcg gacgtctgag
     1021 ccccgaaccg acggtcccta gaaactttcc aggaaatcct gccgggacag gtcctcccag
     1081 gactgccggg cctggccgcc ctgcatgaac ttctcgagcg tcccgaggtc gaggtacttc
     1141 accgtcaggg ggcgctccag ggacaggcgg acgctaccgt cggaccagac gctctcccgg
     1201 ggcgagaggg atcccggctt tccgtaccgg tcggagagcc gggtaaatac cgaatagtgg
     1261 tccacccttt ccgggttcag cacgtagatc gcggagaaga gcttgtcctc gtggaactgg
     1321 aagaaggccc gcttcacgta ggagagtccc tggacctcca ggagcttctc gtccggccgt
     1381 ggcaggaggg tgacatccgg tccgccccgg tacaggaaga gttcgtccgc cttgaggacg
     1441 gtttccacct ccgaaaggga catgccgagc tcgacccccc ggaaaccccg gggcaggccc
     1501 gaggaagggg cggacgccgg ggatgctggc gcgggccccg gggcggaggg ggtcggctgg
     1561 ccgatgaccg gaagggccgc cgccagggcg aaggccagga tcgtccggat gcgggccgcc
     1621 ggggcgggac ggggttgtgt tctcaaggca gcattcatac cgggcacctc acttcgaaag
     1681 ggcgatggac agggccgtgg agagcttgag gacgtcctcg ttgcccttcc gcaggcgcgc
     1741 gcccaggacg gcggcgatcc ggcgaacgac cttcagtccg aggtcgggat ccgcgtcgca
     1801 gagggcggcg aagtcctccc ggcggatccg gtagagcaga cagtccgaag ccgcggtgac
     1861 cgtggcgctc cggggttcgg tcccgaagag ggccatctcc ccgaagaccg aggcctggcc
     1921 ggcgttcagc ttggtcatgg acttctccgc ccggccgaat cccgtccggc ctatcttcag
     1981 ggtcaggttc ttgctgacgt ccacccgccc ctccatgaag aggaacacct cgtcccccgg
     2041 ctcgtcctcc cgcatgatga cggtgccggc cttgacctcc aggggctccg cgatctccag
     2101 gatccttccg agctgttcct cggacaggtc ctgcaggatc ccgaccttcc ccagggccgc
     2161 cgcgttcttg gccgccttcc tcatcatcgg gcccccgatc ccaccgtgaa ggcccagtcg
     2221 gtgtcccgga tgacgtagtc cggcccgggg gccagccgga tctcgttccc ggacccggcc
     2281 tcccgggaca ggtccatctc ggcctcctgg aacttccgct tgatgaaggc gtcgatcgcg
     2341 ctggagtcgt cggacaggag gtcctccagg gccatgccct ttcccttgga caggactccc
     2401 accaggaccg cggatttctc ggacaggtac cagcccgagg cctcccggaa ggtcctgccc
     2461 accagggccg gggggatggg ggactggcgc agccgggatt cgccggagaa gagcctgcgg
     2521 gccgcgcccg gaagtcccgg ctcgaagccg gcggcggata ggagacggcc cgcgaactcg
     2581 ccgttcacga tgacgtcgtc cgcctgggcc cggaccaggt gctgctcgct ctcggtcttc
     2641 aggatttccg cgctggcgtg gatctcggga ttcagggcct tgaccgccag gcaggccatg
     2701 atcgtccgct cgtccgcgtt ggccgtcccg ccggccccgg actcgtcggg caggaggatg
     2761 caagcccggg cggtccccgc agaggccctt cggagcacgg tttcctgggt gtggtccccc
     2821 cggacgaacc ggaggtccag tccggggaac tgggtcttca aatcgtcgaa gaggtcgctc
     2881 tccatctggc agaccaggac gacgggggtg cgagcggcct cctcggatcc cgagatgccc
     2941 tccaggacgt tccgggcctt ggcattccac ccgcagacca ggatatggcg cttcagcttt
     3001 acgtcccgca aacctttacc ctcccgtatc ctgcgctcca cgaagattga ggcgatcgtc
     3061 ccggagatca gggacgtcag gaccactccg gacaggatga ggaggatcgc gaaggacctc
     3121 ccgacgtccg tccggggata cttgtccccg tagccgaccg acgcgatggt gaccacggac
     3181 caccagatcc cgtcgaagag ccgggcgaac atcccgtcct cccagcccga ctccgccagg
     3241 tgcaccagga cggctcccgt cacgacggcg gacaggaaaa acacgagcag ccgcgggagg
     3301 ttggcccggg cggcgtcgtt cttcaggctg gaccaccggt ccgaaaggcc cgagaggaga
     3361 gtcctgccct cggggtcggt cccgtgccgg gcgcggggcc tccgggaatc ggttcgtgtg
     3421 cgtgtcggca tcctggatgg tttcctcctt atcctatcgg ctgatatcgg tcccgtattg
     3481 acctccccgc gcggcgggat tgacggcttc cccgggacgg gaggatattc gggcatgagc
     3541 tccttcgact ttccgacccg gggcggggat ccccgggtcg tccgggcgga tccggacttc
     3601 ctggcggtct ccaagcccgc ggggatgcac acggcccccg cccccgccgg agatcccgac
     3661 ccgtcctctc ccgacctggc ttcctgggtc ttcgagcggt acccggagat ccgggggatc
     3721 cggggccggg accgcggaga ggggggactt ttacaccgat tggaccggga gacctcgggg
     3781 ctcgtcctgt tcgcccgaac cgaggagtcc ttccgggccc tgtccgccgc ggccgcctcg
     3841 gggctcttcc gcaaggacta catcctccgg gcccgccctt cccggtcggg gctcccgggt
     3901 tcacggcccg ttcggaccgc gccggggggg gtggatccgg aggcctgggc cgcctggctt
     3961 gaccggggaa ccccgcccgg ggcgctcggg gggaccgatt gcgccccggg tgccgcggag
     4021 ggccttcggg gcatccttgc cggcgccctg gagtccgggc gtccggtctt cgtggaaagc
     4081 cggttccgcc ccttcggacc cggagccgcc cgggtcgcct gcgccgccgc ggacgcggac
     4141 ctcggccggt cccgggggga ctggggcctc gagacgtacc gaaccgacat cctcgaaacc
     4201 ggcaggacgg gatccgtcct ggaggtacgg gtctccctga cccggggatt ccggcaccag
     4261 gtccgcgccc atctggcctg gatcggcctg cccctggagg gggacggcct gtacgggggc
     4321 ggggaagggg agcggctggc cctgcacgcg gcggccctgg actttccccg tccgggcacg
     4381 ggcgagcgga tccgggtgga ggaccggggt gtccaataag ccggttacgt attggacaac
     4441 tcgccggatt gcgcccggcc ggggcatgga ttaggatagg ggtagtatga gcgaaccctc
     4501 gaccttcgac agcctggttc ggggcctttc ccgggaagaa cggcgggacc tcctcacccg
     4561 gatccggggg accgccgagg tgtccgacga accgatgttc cggccggaga ccgtgcggga
     4621 acccccggtc gactattccg cccagtaccg ggagctcggt cccctggcac ggctggtcat
     4681 cctggtccgg aggctgttct cggggaagcc ccgggacgaa ctggtcaagg aactcctgct
     4741 caggcacttg gcgcgccgga tcgagctggg gagcccgggg ctggtgtcct attcccgccg
     4801 ggtcttcctg aaccgcatgc tcgcggagct ccgggacctc cgggacgggg cgcggtacat
     4861 ctacgacctc ctggacaggt cggtcgaaaa ggaccgggga gctttctatg ccttcctggc
     4921 ctccctggaa ctggaggaac agcaccaaag gctcctttcc gagacggatc ccttcctttt
     4981 tgcccaaacc aattccctgg cgggggagcc cgaagtgcgg acggccgtct ggaccgcctt
     5041 cgacggcatc ctttcctcga tgcccgagga gagccgccgg aggatgtacg ccgacgtccg
     5101 gagcctcgcc ttcctgaagc gggtggcggg attcctgtac gaccgcctgt tctcctgctt
     5161 cgagggggcg ggcggggccg ccccggaaag ccccttcgtc accgcccggg acctcatgtc
     5221 cgagctcgcg gacatcctct actccatggc ctccccgccc tccctgaagc tcatggaatc
     5281 cctcctggtc ttccagatgc gggaggagat ggagaacccc gatttcgacg ccgagacggc
     5341 cttgaagacc caactggccc gggtggagga ggccctggcc cggatccggt ccttcaacgc
     5401 ccgggttccc ctccacgaga tcctgcgggt ggtgatggag gaccccaact accggccccg
     5461 ggaactcgcg ggcggggagg actggttcgc catctacaag ggattctgga aggagaggat
     5521 cgagaaacag ctgtccctct acgggacggc ccggaggatg caggacctcc agtccgacat
     5581 agcgggcttc ctgggatcat cggagccggt ccggttcgag tacatcaacg aggagggcag
     5641 ggaaggcagc cccccggtgc ggctggaaaa agctttgggc ttcctctcgg ccttctacga
     5701 gcggctcttc gtgcccgaga tgaaccggac cctcaagatc ctcctgatcg agggggagtt
     5761 ctacaagaag gacaaccgcg tggagtacac ggacgcgtat aacgagctcc tacagatccc
     5821 ggaggccctg tccggactgg acgcccggct cggccccggc ggggacctcg gttcggcctg
     5881 ggtccaggcc aaggaggagc tggtatccct gcccctcaag cgccggaagg tccaggcggc
     5941 cctccaggcc gcgaacgggg aggcggaagc cttgctcagc cgcagccggg aggcgatgaa
     6001 gcggatgcag gccatcctga agggccttct caagggggag gccgggggac gctacgattc
     6061 cctgtccaac ctttcctaca tcgagggcaa gggcaacaag gaattcctgc ggaacctgga
     6121 ttccatcaag aaccgcctgg agcggacggt ccacttcctg gatgagctcg agaagctggg
     6181 tatgacccgg gagccggcgt gacctgcacg gaactgtccc tcgactcggt ccggtccggc
     6241 ggcttcgccc gctttcccct gggcgcggat cccggagccc cctgggccct cttgtccctg
     6301 agacccctgg gatccatgcg cttcctccac gaccggccga accccctgcg ggaggacttc
     6361 ttccgggacc ggggaatcga tcccggccgg gtcctggcgg tggagctggt ccattcccgg
     6421 acggtcctct tccccaaggg ccccgcggag ctccggggaa tccgggcgga cggcctcatc
     6481 gtggagcccg gaccctgggt ccccacggtg accgtggcgg actgcatgcc catctggatc
     6541 cgccacgctc ccagcggggc ctacgggatc ctccactcgg gctggcaggg caccggcatc
     6601 ctcgccgccg cggtccgggg actcgaggaa cgcttcggag cccgcccctc cgagatcgac
     6661 gccttcctgg ggcccgccat cggcccctgc tgttaccggg tccccgagga ccgagcccgg
     6721 ggcttccggg ccgaattcgg tcccgaggcc gcgaggctcg aggacggagc ctggagactg
     6781 gacctggggg ccgccaacct cggtatcgca cggtccttgg gcctccggtc cgtgacccgc
     6841 atctccggct gcac
//
LOCUS       DAOTEI010000186         6803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_7991_length_6803_cov_4.146858, whole genome shotgun sequence.
ACCESSION   DAOTEI010000186 DAOTEI010000000
VERSION     DAOTEI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7991_length_6803_cov_4.146858"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="P5313_14365"
     CDS             complement(<1..813)
                     /locus_tag="P5313_14365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY81597.1"
                     /translation="MFTDHDWMTQLGSSVRDAGGLALRVSLDPETITAIDCLSEGGGL
                     PLRVTPYWLSLAGPGPDDPILAQCLPSPRESEPDPDAVPDPLGESLHRASPSLVRQYR
                     SRALLRASGECAVHCRHCFRRNLLPSERGYISPEDQAAAAAWLAGHPEIREILVSGGD
                     PLVASDARIEGLLSALRAARPEAVLRICTRCPSTLPMRFTPALTALLRSFRPLRVVTH
                     FNHPRELSPQADEALELLIDAGIPVSVQTVLLAGINDDPEVLETLFAGLARRG"
     gene            complement(961..>1560)
                     /locus_tag="P5313_14370"
     CDS             complement(961..>1560)
                     /locus_tag="P5313_14370"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRY81598.1"
                     /translation="IGHQKGFLKAGGVLRAKFAQNCRVAVRGDVFIPGSLVSCRLFTL
                     GMLEMGDKGRILAGETFAVHGVRCGWIGSDTGQTTYVHVGIDFTVQQRLDLANERLRL
                     IQMKRDKLEELGKTRPSAKLEALKQELDKAASELSALISDLLPKLDADERAFVEIRGG
                     AFPGAVIEICRVRFQIQEEIKKTVFRLDKASGRIIQDHT"
     assembly_gap    1561..1570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1571..2360)
                     /locus_tag="P5313_14375"
     CDS             complement(<1571..2360)
                     /locus_tag="P5313_14375"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRY81599.1"
                     /translation="MESKSPEKKAAKPREMKLSFSPGGRKNDGFVEISVPEDAMEVQA
                     SFHPAVGGGAPLSQEYVRELLSRAGVTEGILEDVIGDSVLSCNLDQHPLREVVIARGV
                     PAEEEIPGHLKLLKRFEETPLDAAVPDGSLDWKDLSPVIVVTRGERIADKISARAGTP
                     GKTVRGEAIPAKKRSVTQYLPGRNVRMDGEALVADVDGRFSLSGNRIDVEEVLVAKGG
                     VGYHTGHIVFPGDVTIEGPVRDGFKVHSGGSILAKDTMDVFDVNA"
     gene            2511..2996
                     /locus_tag="P5313_14380"
     CDS             2511..2996
                     /locus_tag="P5313_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HRY81600.1"
                     /translation="MKDSKERSERLKAILRLIRAHRIESQESLLKFLEAEGFILTQAT
                     LSRDLKALKVGKISAGESGYYYSVPSDEERRETDASFIQDLQRGYVSADWNSSLVVVH
                     TFSGHSATVALALDSLGFECSLGTLAGQDNAVFIALRQGYTGEQFVEELHRKVPSLRA
                     E"
     gene            complement(3050..3253)
                     /locus_tag="P5313_14385"
     CDS             complement(3050..3253)
                     /locus_tag="P5313_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HRY81601.1"
                     /translation="MEQRVILLHGFEGESLIAAVRAIKAVLPDPAGIAFASTTPVNVD
                     WKVGDLIEHVAEEHEAFRQSRTS"
     gene            3383..5962
                     /gene="clpB"
                     /locus_tag="P5313_14390"
     CDS             3383..5962
                     /gene="clpB"
                     /locus_tag="P5313_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRY81602.1"
                     /translation="MDYDRYTTKTQEVLQAATTLARQNDHGQIDTDHLLAALLEQEDG
                     VVSPLLGRLEVDVPGLLEASRSLIAKKPKVFGEAAQVYLSPAASKVFAKAEGEAKALK
                     DDYVATEHLLLALIGDEGPVGSLLRDRGIVKTAVLEALKAVRGTRRAADPEAEAKYQV
                     LERYCRDLTALARAEKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LARRIVAGDVPDSLRNKKLLALDLGALVAGSKFRGEFEERLKAVIEEIQKSDGKIVLF
                     IDELHTLVGAGAAEGAMDASNLLKPALARGELKAVGATTLDEYRKHIEKDAALERRFQ
                     PVFCGEPSVEDTIAILRGLAERYEVHHGVRIRDEALIAAATLSDRYITSRFLPDKAID
                     LVDEAASRIKMEIESQPTELDQAERRILQLTIEKQALTKETDPGSKERLAKLEKELAE
                     VRGRRDSMKLRWTSEKGKIEEIRKLKESLERLKIEEQRYERDGNLAKAAEIKYGRLLE
                     AEKRLAEVSAEIGKQDDGTRLLREEVSEEDIARVVSTWTGIPVSKMLSGEAQKYLELE
                     KVLGRRVLGQQAAIKAVADAIRRNRAGLSDPNRPLGSFLFIGPTGVGKTELAKTLAEF
                     LFNDEKSLTRIDMSEYGEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLFDEI
                     EKAHPDVFNVLLQLLDDGRLTDGQGRVVDFKNAIVILTSNLGSDLILAAESMKAVKPA
                     IDQLLRVSFKPEFLNRIDETVLFERLGKAEILGIVDLQIALLAKRLESRKIALEVSPE
                     AKAWLAEAGYDPLFGARPLKRAVQNELQNPLARDVLAGKIREGSRVKVALSGGKLAFG
                     TAS"
     gene            5959..6789
                     /locus_tag="P5313_14395"
     CDS             5959..6789
                     /locus_tag="P5313_14395"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRY81603.1"
                     /translation="MTAAAPGGPCTDSHAHLSMIPARAGRAALEGVLAAYDAAWEAAA
                     GVPGRAPRVVDPGTRADDLPERVRALGRRPWLRFSAGIWPGPEALRLPEPHIAALEAA
                     ASSPDCAAIGEGGLDYHWMHGSREAQRLLFEAQIDLARRLGKPLIVHSRDAHGDTVET
                     LRSARPGIPVVIHCFGYDAAAARDYLDLGCFVSFAGNLTYPAARNLREACALVPRDRL
                     LLETDSPYMAPVPERGKPCTPLLVGHTYAFAAELRGEDAGDLARAVTENTVRIFFGPQ
                     "
BASE COUNT         1153 a         2312 c         2315 g         1013 t
ORIGIN      
        1 ccccctccga gccagccccg caaagagggt ttccaggacc tccgggtcgt cgttgatccc
       61 ggcgaggagg accgtctgca ccgagacggg gatgcccgcg tcgatcagga gctccagggc
      121 ttcgtccgcc tggggggaaa gctcccgggg gtggttaaag tgggtgacca cgcgcagggg
      181 ccggaaagaa cgcagaaggg cggtcagggc gggggtgaat cgcatgggga gtgtggaagg
      241 acaacgcgtg cagatccgaa ggaccgcctc cggccgggcg gcgcgcaggg cggataaaag
      301 gccttcgatc cgggcgtccg aggcgaccag aggatccccc ccggagacca ggatctcccg
      361 gatctcgggg tgcccggcca gccaggcggc ggccgcggcc tggtcctccg gggagatgta
      421 gccacgttcc gagggcagga ggttcctgcg gaagcagtgg cggcagtgga ccgcgcactc
      481 cccggacgca cgcagcaggg cccgggatcg gtactgccgg accagggagg gcgaggcgcg
      541 gtgcagggac tcccccaggg gatccgggac ggcatcggga tcgggttccg attcccgggg
      601 cgagggcagg cactgggcca ggatggggtc gtccggtcct gggcccgcca gggaaagcca
      661 gtagggggtt acgcggaggg ggaggccgcc gccttccgag agacagtcga tggcggtgat
      721 cgtttccgga tccaaggaaa cgcggagggc cagacccccc gcgtcccgga cggaggagcc
      781 cagctgggtc atccagtcat ggtcggtgaa caagatgtca aagagtaccg aaagctcggg
      841 atcgcggcaa tacgggagac ggcgggtccc gccgggcagg ccgcgggtcg tacgcgggcc
      901 ggatgtccgc ggcgggacgt tcgtcgaaca aggggccggg catcctgccg gccccgcgat
      961 tcaggtatgg tcctggatga tccgccccga ggccttgtcc agccggaaga ccgtcttctt
     1021 gatctcctcc tggatctgga accggacccg gcagatctcg atcaccgctc ccgggaacgc
     1081 cccgccgcgg atctccacga aggcgcgctc gtccgcgtcc agcttgggca ggaggtcgga
     1141 gatcaatgcg gagagctccg aggccgcctt gtccagctcc tgcttgaggg cctccagctt
     1201 ggcgctgggc cgggttttgc ccagctcttc gagcttgtcc ctcttcatct ggatcaagcg
     1261 taaccgctcg ttggcaaggt ccagcctctg ctggaccgtg aaatcgatgc cgacgtgcac
     1321 gtacgtggtc tgtccggtgt ccgagccgat ccatccgcag cgcaccccgt ggaccgcgaa
     1381 ggtctccccc gccaggatcc tgcccttgtc ccccatctcc agcatgccca gggtgaaaag
     1441 gcggcaggag accagggagc cgggaatgaa gacgtccccc cgcacggcta cgcggcagtt
     1501 ctgggcgaac ttggcccgga gcacgccgcc cgccttcagg aaacccttct ggtgcccgat
     1561 nnnnnnnnnn tggcgttcac gtcgaagacg tccatcgtgt ccttggccag gatggagccc
     1621 ccggagtgga ccttgaatcc gtcccggacg ggcccctcga tggtgacgtc cccggggaag
     1681 acgatgtggc ccgtgtggta gcccactccg cccttggcca ccaggacttc ctccacgtcg
     1741 atccggttgc ccgagaggga gaatcggccg tccacgtcgg ccaccagggc ctcgccgtcc
     1801 atgcgcacgt tccggcccgg gagatactgg gtcacggacc tctttttcgc ggggatcgcc
     1861 tcgccgcgga cggtctttcc gggggtgccc gcgcgggccg atatcttgtc cgcgatcctc
     1921 tcgccccggg tgaccacgat gacgggggac aggtccttcc agtccaggga cccgtcgggt
     1981 acggccgcat ccaggggcgt ctcctcgaac cgcttcagga gcttgaggtg ccccgggatc
     2041 tcctcctccg cgggaacgcc ccgggcgatc acgacctccc gcaggggatg ctggtccagg
     2101 ttgcaggaca ggaccgagtc cccgatgacg tcctccagga tcccctccgt cacccccgcc
     2161 cgggaaagga gttcccggac gtactcctgg gagagggggg cgccgcctcc gacggcgggg
     2221 tggaaggaag cctggacctc catggcgtcc tcgggcacgg agatctccac gaatccgtcg
     2281 ttcttgcgcc ctccgggact gaaggagagt ttcatctccc ggggtttggc ggctttcttc
     2341 tctgggctct tggattccat cctgatatat gcccccccat tattgtatcg gcataccacc
     2401 cggaaatcac caacaaatcc gtcctaggtc gatggtgtcc gtcatcgagc ttgcaagaac
     2461 ctcgtacccg tggcatgata cgaaaacagc aacaaaccga ggtcgggatc gtgaaagact
     2521 ccaaggaacg atcggaacgt ctcaaggcga tattgcggct gatccgggcg catcggatcg
     2581 aatctcagga aagccttctc aaattcctgg aagccgaggg attcatcctc acccaggcca
     2641 ccctttcccg ggacctgaag gccctgaagg tggggaagat ctccgcggga gaatccggat
     2701 actactactc cgtcccctcc gacgaggaac ggagggagac cgacgccagt ttcatccagg
     2761 acctccagcg gggctacgtc tccgcggact ggaattccag cctggtcgtg gtccatacct
     2821 tctccggcca ttcggccacc gtggccctgg ccctggacag cctgggcttc gagtgttccc
     2881 tgggcaccct ggcgggccag gacaacgccg tgttcatcgc ccttcggcag ggctacacgg
     2941 gggagcagtt cgtggaagag ctccatcgca aggtcccgag cctccgggcc gagtgaaccg
     3001 cccggctccg atcgccggcg caccgggccc gatccgggcc gccccgtcct taggaggtcc
     3061 ggctctgccg gaaggcctcg tgctcctcgg ccacgtgctc gatcaggtcc cccaccttcc
     3121 agtccacgtt gaccggggtc gtcgacgcga aggcgattcc cgcgggatcg ggcaggacgg
     3181 ccttgatggc cctcaccgcg gcgatcaggg actcgccctc gaagccgtgc aacaaaatca
     3241 cgcgttgttc catgccccat cctaacgtaa aacgtgctat cgtgccataa actcgacaaa
     3301 gcctcctagg cttcgtcgcc gcgaaccgcg tcgcggttcg cgcccttccc gctcctcctt
     3361 cggcggaggt atattccatc tcatggacta cgatcgctac acaaccaaga cgcaggaagt
     3421 cctgcaggcc gccacgaccc tggcacggca gaatgaccac ggtcagatag acaccgacca
     3481 cctcctggcg gccctcctgg agcaggagga cggcgtggtc tcccccctcc tgggacgcct
     3541 ggaagtcgac gtgccgggcc tcctggaggc ctctcgctcc ttgatcgcga agaaacccaa
     3601 ggtgttcggg gaggccgccc aggtctacct ctccccggcc gcttccaagg ttttcgccaa
     3661 ggccgagggg gaggccaagg ccctgaagga cgactacgtg gccacggagc acctcctcct
     3721 ggccctgatc ggcgacgagg gacccgtggg aagcctcctc cgggaccggg ggatcgtgaa
     3781 aaccgcggtc ctggaggccc tcaaggccgt gcgcggcacc cggcgggccg cggatccgga
     3841 ggcggaggcc aagtaccagg tcctggagcg ctactgccgg gacctgaccg ccctggcccg
     3901 ggcggagaag ctggaccccg tgatcggccg ggacgaggag atccgccggg tcatgcaggt
     3961 cctgtcccgg cgcaccaaaa acaaccccgt cctgatcggg gagcccgggg tcggcaagac
     4021 cgccatcgtg gagggcctgg cccggcgcat cgtggccggg gacgtgccgg acagcctgcg
     4081 gaacaagaag ctcctggccc tggacctggg ggccctggtc gcgggatcca agttccgggg
     4141 ggaattcgag gagcgcctga aggccgtgat cgaggagatc cagaagtccg acgggaagat
     4201 cgtcctcttc atcgacgagc tccataccct ggtgggggcg ggtgccgcgg agggggccat
     4261 ggacgcctcc aatctcctca agcccgccct ggcccggggg gagctcaagg ccgtcggtgc
     4321 cacgaccctg gacgaatacc gcaagcacat cgagaaggac gccgccctgg agcgccgctt
     4381 ccagcccgtg ttctgcgggg agccctccgt ggaggacacc atagcgatcc tccggggcct
     4441 cgcggagcgc tacgaggtcc accacggcgt ccggatccgg gacgaggccc tgatcgccgc
     4501 ggccaccctc tcggaccgtt acatcaccag ccgcttcctg ccggacaagg ccatcgacct
     4561 ggtggacgag gcggccagcc gcatcaagat ggagatcgag agccagccca cggaactgga
     4621 ccaggcggag cgccgcatcc tccagctcac gatcgagaaa caggccctta ccaaggagac
     4681 ggatccgggc tccaaggagc ggctggccaa gctggagaag gagctggcgg aggtccgggg
     4741 ccgcagggat tccatgaagc tccggtggac ctcggagaag ggcaagatcg aggagattcg
     4801 gaagctcaag gagagcctgg agcggctcaa gatcgaggaa cagcgctacg agcgggacgg
     4861 caacctggcc aaggccgcgg agatcaagta cggcaggctc ctggaagccg agaaacgcct
     4921 ggccgaggtg agcgcggaga tcggcaagca ggacgacggg acccgcctcc tccgggagga
     4981 ggtctctgag gaggacatcg cccgggtggt ttccacctgg accggcatcc ccgtgtccaa
     5041 gatgctctcc ggggaggccc agaagtacct ggagctggag aaggtcctgg gccggagggt
     5101 cctgggccag caggcggcga tcaaggccgt ggcggacgcc atccgccgga accgggcggg
     5161 cctctcggac ccgaaccgcc ccctcggaag tttcctcttc ataggcccca cgggggtcgg
     5221 caagaccgag ctggccaaga ccctggcgga gttcctcttc aacgacgaga agtccctgac
     5281 ccggatcgac atgagcgagt acggggaaaa gcactccgtg agccgcctga tcggagcgcc
     5341 cccgggctac gtgggctacg acgagggcgg ccagctgacc gaggcggtcc gccgccgacc
     5401 ctacagcgtc gtcctcttcg acgagatcga gaaggcccac cccgacgtct tcaacgtcct
     5461 tttacagctc ctggacgacg gccgcctcac ggacgggcag gggagggtgg tggacttcaa
     5521 gaacgccatc gtcatcctga ccagcaacct gggcagcgac ctcatcctgg ccgccgagtc
     5581 catgaaggcg gtcaagcccg ccatcgacca gctcctccgg gtcagcttca agcccgagtt
     5641 cctgaaccgg atcgacgaga ccgtcctgtt cgagcgcctc gggaaggcgg agatcctggg
     5701 catcgtggac ctgcagatcg ccctcctggc caagcggctg gaatcccgga agatcgccct
     5761 ggaggtgagc cccgaggcca aggcctggct cgccgaggcg ggctacgatc ccctgttcgg
     5821 ggcgcgcccc ctcaagcggg cggtgcagaa cgagctccaa aatcccctgg cccgggacgt
     5881 cctggccggg aagatccggg aagggagccg cgtgaaggtg gccctctccg gcggcaagct
     5941 ggctttcggg acggcctcgt gaccgcggcg gctcccggag gaccgtgcac cgactcccac
     6001 gcccacctct ccatgatccc ggcccgggcg ggccgggccg ccctggaggg tgtcctggcg
     6061 gcctacgacg ccgcgtggga ggccgcggcc ggggttccgg gccgggcccc ccgggtcgtg
     6121 gatcccggca cccgggcgga cgaccttccg gagcgggtcc gcgccctggg ccgccgcccc
     6181 tggctccgtt tctccgcggg gatctggccc gggccggagg ccctccggct gccggaacct
     6241 cacatcgcgg ccctggaggc ggccgcctcc tccccggact gcgccgcgat cggggagggg
     6301 ggcctggatt accactggat gcacggcagc cgggaggcgc agcgcctact cttcgaggcc
     6361 cagatcgacc tggcccggcg cctgggcaag cccctgatcg tacattcccg ggacgcccac
     6421 ggggacaccg tcgagaccct ccgttccgcg cggcccggca tccccgtggt gatccactgc
     6481 ttcggctacg acgcggccgc ggcccgggac tacctggacc tggggtgctt cgtctccttc
     6541 gcggggaacc tgacctatcc cgccgcccgg aacctgcggg aggcctgcgc cctggtgccc
     6601 cgggaccggc tgctcctgga gaccgattcc ccctacatgg ccccggtccc ggaacgggga
     6661 aagccctgca cgcccctgct ggtcgggcac acctacgcct tcgccgcgga gctccggggc
     6721 gaggacgcgg gggacctggc gcgggcggtc acggagaaca cggtcaggat tttcttcggg
     6781 cctcagtagc cggacaatcg ttt
//
LOCUS       DAOTEI010000187         6722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_8117_length_6722_cov_4.152242, whole genome shotgun sequence.
ACCESSION   DAOTEI010000187 DAOTEI010000000
VERSION     DAOTEI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8117_length_6722_cov_4.152242"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="P5313_14400"
     CDS             <1..864
                     /locus_tag="P5313_14400"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRY81604.1"
                     /translation="TGSWKNVRFRAYNVRGPAARVLPGRRNPYMEFLEGVKDKLVSLG
                     FQEFDGSLVETEFWNSDALFMPQFHSARDIHDVYHLAEPTHAREIEEPFLSNVAAAHE
                     NGGETGSRGWGYGFDRDFTRRLILRSQGTVLSARTLPKAEIPGRYFGMARCFRYDKVD
                     ATHLSDFYQTEGIVLGPDVNLRTLLGFLEMFAVEVAGAKEVKYVPGYFPFTEPSVEVH
                     IKHPVLGWFELGGSGIFRPEVTEPLGVHVPVLAWGIGIDRMALMALGLNDLRELFSPD
                     IESVRLRRAKI"
     gene            886..2595
                     /gene="pheT"
                     /locus_tag="P5313_14405"
     CDS             886..2595
                     /gene="pheT"
                     /locus_tag="P5313_14405"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRY81605.1"
                     /translation="MPKIDVNEKLFFDLAGRKYSAEELELALQGAKAELDGWETGAGV
                     APEERTIKIELNDTNRPDLWSTAGIARQLRTLAGGAVPSYPFFSREGDLRPATRRVVV
                     DPSVLGVRPFMAAFVISGKPITDSMLRDAIQTQEKLCWNFGRKRASVSMGIYRTSLIQ
                     WPVHYKAVPPASVRFVPLQMTEALNLSEILDKHPKGQEFGFINRGKKLHPLLTDSRGG
                     VLSYPPIINSADIGAVQVGDTDLLVELTGTDLASVTLSASIVACDFADAGYTIEPVAI
                     DFPEPTEFGRTVVFPYYFQKPVSVDSGRAGRLLGRSLSPAAVAEALARMGVRSETHGQ
                     FVTAFPPEYRNDFLHPVDIIEDVMIGVGMPGFQPERPKDFTIGRLTPVETLARKAKAV
                     LVGMGYQEMVYNYLGSGRDYVERMNIPGDAVLRIANPMTENFEYVRPSILPCLLGSES
                     VSGKAVYPHRILEAGKVAFKDESANYGSVTRTYLGFLSSHGEANYNEAASLVSALLYY
                     LGAEYSVEECEDPRFIPGRQARILRNGAGVGVFGEIHPRVLENWGITVPCVAGELDLE
                     AYL"
     gene            2632..4392
                     /locus_tag="P5313_14410"
     CDS             2632..4392
                     /locus_tag="P5313_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY81606.1"
                     /translation="MDYKTYFAEHPGWHYGNFARMLAGTVRAYGDSTAFRARPAGKEE
                     GYETLSFRELESRVRNLSRFFVAAGLSAGDPVAIYSENRPEWCVCYFAAVIAGLVAVP
                     LDTALDERGAANILAASSARVLAYSARQRDKARAAAAISGIAGLVLLDLDREDDGTAS
                     DGVLERSYRRIQAEPPSADLPDPDSIPGDATAAIIFTSGTTGVAKGIVLSHAGILANM
                     EASIRALRVDGRDNILSVLPLHHTYATTCTFLAPLEAGCSVTFVEKIVPSVVLRHIEE
                     SRVTVLIGVPLLFDKIRAGIEGELRKLPAAAGWMLRALIGAARFLTVDCGLDAGRPLL
                     GFLRRKAGLDSLRLCVSGGGPLLPATAHFFDALGLRMVQGYGMSENGPLISVNLPRFK
                     DNRSVGVPVWNTEVRIADAGPDGIGEIQVKSPSLMKGYLNAPEATAGTLTPDGWLRTG
                     DLGRVDGRGFIFITGRSKDLIVTEGGKNVYPEEIEQRFSDSPWIAEVLVLGRGRSEGH
                     SGEDVVALCHPNFDAIRAAHPGREGDPEFVGSLVRSEVQRVNKSLPPYMKVVYHRIRD
                     TEFEKTSTRKIKRFLYKDSF"
     gene            4580..4879
                     /locus_tag="P5313_14415"
     CDS             4580..4879
                     /locus_tag="P5313_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81607.1"
                     /translation="MDTAAPVLLVSRLTLTAVTTFLAIVLWSRTRDLAWMLMVVGTVA
                     GYADILYGLLFRFGVVPEGPLAAPGVPLAVLILSNLPTLLYGAAFAVMVARKRLR"
     gene            5009..6589
                     /locus_tag="P5313_14420"
     CDS             5009..6589
                     /locus_tag="P5313_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRY81608.1"
                     /translation="MEVYAFAPTGFEGGLVRVEVDIRRGLPAFDLTGLPDDAVRESRD
                     RVRAAIRNAGFEFPLDRVLVNLAPADLRKSGASFDLPIALGILAESGQVPEAGRPVLA
                     LGELGLGGDIRPARGALPAVAAGLSAGIEDFAVPRENLAEARSLGRGRVRGVSSLGEF
                     CVLFLPAGALAEGAPCPECAGTPADPDADSRTAASWTAQDAGGPEGDFSDIRGQPQLR
                     RALEVAAAGGHHVLLFGPPGVGKTLAARRLPTLLPDLSGTAAVDVTRIHSLAGLLGPG
                     TGLLRRPPFRSPHHGASAEGVVGGGRCARPGEISLAHGGVLFLDEAPEFRSDILQALR
                     EPTEEGKISIARAEATLSFPARFQLVLAANPCPCGSLGRPGAVCLCSIQDLRRYWKKL
                     GGPLMDRIDIRVPVKPAPFRDLQAPEEPSSAVRLRVERARRIQAERFEGLDCEVNGRI
                     PAGFVGRYCPVDATTTAFLDRQSEALGLSSRALHSILRVSRTIADLDGAASISLRHAE
                     EAAELRRYGDEDPFWRIP"
BASE COUNT         1031 a         2334 c         2225 g         1132 t
ORIGIN      
        1 acgggatcct ggaagaacgt ccgcttccgg gcctacaacg tacgcggccc cgccgcccgg
       61 gtcctgccgg gaaggcggaa tccctacatg gagttcctgg aaggggtcaa ggacaagctc
      121 gtgtccctgg gcttccagga gttcgacggg tccctggtgg agaccgagtt ctggaactcc
      181 gacgccctgt tcatgcccca gttccactcc gcccgggaca tccacgacgt ctaccacctg
      241 gcggagccca cccacgcccg agagatcgag gagcccttcc tgtccaacgt ggccgcggcc
      301 cacgagaacg ggggcgagac cggcagccgg ggctggggct acggcttcga ccgggacttc
      361 acccggcgtc tgatcctgcg cagccagggc acggtgctct ccgcccggac ccttcccaag
      421 gcggagattc cgggccgcta cttcggcatg gcccggtgct tccgctacga caaggtggac
      481 gccacccact tgtccgactt ctaccagacc gagggcatcg tcctgggtcc cgacgtcaac
      541 ctgcgcaccc tcctggggtt cctggagatg ttcgccgtgg aggtggcggg ggccaaggaa
      601 gtcaagtacg tacccggcta cttccccttc acggaaccct ccgtggaggt ccacatcaag
      661 catcccgtcc tgggctggtt cgagctgggg gggtccggca tattccgacc cgaggtgacc
      721 gagcccctgg gggtgcacgt gccggtcctg gcctggggga tcgggatcga ccggatggcc
      781 ctgatggccc tgggcctgaa cgacctccgg gagctcttca gcccggacat cgagagcgtc
      841 cgcctgcgcc gggccaagat ctagcggaag acagaggata gcgatatgcc caagatagac
      901 gtcaacgaaa agcttttctt cgacctggcg ggccggaaat actcggccga ggagctggag
      961 cttgccctcc agggcgcgaa ggcagagctg gacggctggg aaaccggcgc cggagtggcc
     1021 cccgaggaac ggaccatcaa gatcgagctg aacgacacga accggccgga cctctggtcc
     1081 accgccggga tcgcccgcca gctccggacc ctcgcgggag gggccgtccc gtcctatccc
     1141 ttcttctccc gggaggggga cctccgcccg gcgacccgcc gggtggtcgt ggacccctcg
     1201 gtcctggggg tgcggccctt catggccgcc ttcgtgattt ccggcaagcc catcacggat
     1261 tccatgctcc gggacgccat ccagacccag gaaaaactct gctggaactt cggccgcaag
     1321 cgcgcctccg tctccatggg catctaccgg acctccctga tccagtggcc cgtccactac
     1381 aaggcggtgc ccccggcctc ggtgcgcttc gtgcccctgc agatgacgga ggccctgaac
     1441 ctctcggaga tcctggacaa gcacccgaag ggccaggagt tcggattcat aaaccggggg
     1501 aagaagctgc accccctcct gacggattcc cggggcgggg tcctgtccta tccccccatc
     1561 atcaacagcg cggacatcgg ggccgtccag gtgggggaca cggacctcct ggtggagctg
     1621 acgggcacgg acctggcctc ggtcaccctg tccgcctcca tcgtggcctg cgacttcgcg
     1681 gacgcgggct acaccatcga gcccgtggcc atcgacttcc ccgagcccac cgagttcggc
     1741 cggaccgtcg tgttcccgta ctacttccag aagcccgtgt ccgtggattc cgggcgggcg
     1801 ggccgcctcc tgggccggtc cctctccccc gcggcggtgg ccgaggccct cgcccggatg
     1861 ggcgtccgtt cggagaccca cggccaattc gtcaccgcct tccccccgga gtaccggaac
     1921 gacttcctcc atcccgtgga catcatcgag gacgtcatga tcggcgtcgg gatgcccggc
     1981 ttccagcccg agcggcccaa ggacttcacc atcggacggt tgacccccgt ggaaaccctg
     2041 gcgcgcaagg ccaaggccgt cctggtgggc atgggctacc aggagatggt ctacaactac
     2101 ctgggctccg gccgggacta cgtggagcgg atgaacatcc ccggggacgc ggtcctgcgc
     2161 atcgccaacc ccatgaccga gaacttcgag tacgtccggc cctcgatcct tccctgcctc
     2221 ctgggctcgg aatccgtgtc cgggaaggcc gtgtatcccc accggatcct ggaggccggc
     2281 aaggtggcgt tcaaggacga gtcggccaac tacggctccg tgacccggac ctacctgggc
     2341 ttcctgtcct cccacgggga ggcgaactac aacgaggccg ccagcctggt ctcggccttg
     2401 ctgtactacc tgggcgccga gtattccgtg gaggaatgcg aggatccccg gttcataccc
     2461 gggcgccagg cccggatcct ccggaacggg gccggggtcg gcgtcttcgg cgagatccac
     2521 ccccgggtcc tggagaactg gggaatcacg gtgccctgcg tggcggggga gctggacctg
     2581 gaggcatacc tctaacatcg gaggtcccga gcgggggcca aggaacgatc catggattac
     2641 aaaacctatt tcgcggagca tcccggctgg cactacggga acttcgcccg gatgctcgcg
     2701 ggtacggtcc gggcctacgg ggactccacg gccttccggg cgcgtccggc ggggaaggag
     2761 gagggctacg agaccctctc cttccgggaa ctggagtccc gggtccggaa cctgtcccga
     2821 ttcttcgtag ccgcgggcct ttccgcgggg gatcccgtgg ccatctactc ggagaaccgt
     2881 cccgaatggt gcgtctgcta cttcgccgcg gtcatcgccg ggctcgtggc ggttcccctg
     2941 gacacggcct tggacgagcg gggggcagcc aacatcctgg ccgcctcctc cgcccgggtc
     3001 ctggcgtact ccgcccggca gagggacaag gcccgagccg cggccgccat ttccgggatc
     3061 gccggcctcg tcctcctgga tctggaccgg gaggacgacg ggacggcctc cgacggtgtc
     3121 cttgaacgct cctaccggcg catccaggcc gagcctccct ccgcggacct cccagatccg
     3181 gactccatcc ccggcgacgc cacggcggcg atcatcttca cctccggcac caccggggtg
     3241 gccaagggca tcgtcctctc ccacgccggg atcctggcga acatggaggc ctcgatccgg
     3301 gccctgcggg tggacgggcg ggacaatatc ctctcggtcc tgcccctgca ccatacctac
     3361 gcgaccacct gcaccttcct ggcccccctg gaggcgggct gctcggtgac cttcgtggag
     3421 aagatcgtcc cctccgtggt cctgcgccac atcgaggaat cccgggtgac ggtcctgatc
     3481 ggggtacccc tcctgttcga caagatccgg gcgggcatcg agggggagct ccggaagctg
     3541 ccggccgcgg cgggctggat gctccgggcc ctgatcggag ccgcccgctt cctgaccgtg
     3601 gactgcggcc tggacgccgg aaggccgctc ctgggtttcc tccgaaggaa ggccggcctc
     3661 gattccctgc gcctgtgcgt gtccggcggt gggcccctgc tgccggccac agcccacttc
     3721 ttcgacgcct tgggactccg catggtccag ggctacggga tgagcgagaa cggccccctg
     3781 atttccgtga acctgccccg gttcaaggac aaccgctccg tgggggttcc ggtctggaac
     3841 acggaagtcc ggatcgcgga cgcggggccc gacgggatcg gggagatcca ggtcaaaagc
     3901 ccctccctga tgaaggggta cttgaacgcc cccgaggcga ccgccgggac cctgaccccg
     3961 gacggatggc tccgcaccgg ggacctcggg cgagtcgacg gtcggggctt catcttcatc
     4021 accggccgat ccaaggacct catcgtcacc gagggcggga agaacgtgta tcccgaggag
     4081 atcgagcaga ggttttccga ttccccctgg atcgcggagg tcctggtcct gggccgggga
     4141 cggtcggaag gccactccgg ggaagacgtg gtggccctct gccatcccaa tttcgacgcg
     4201 atccgggccg cccaccccgg ccgggaaggg gatccggaat tcgtgggctc cctggtgcgg
     4261 tccgaggtcc agagggtgaa caagtccctg ccgccgtaca tgaaggtggt gtatcacagg
     4321 atccgggata cggagttcga aaagaccagc acccggaaga tcaagagatt cctgtacaag
     4381 gattcgttct aggagtttgt cgggcttgcc caccttaata gtagaaaaat cgcaggtatg
     4441 cgatttttct actttgtaaa ctccaaagga accggataat cgggatatta agcgcctatc
     4501 ggcgcttaat atccacaagt ctgacaggct cctagtcccc cggctttacc gaggggcccc
     4561 tgcggagtag tatatggaca tggataccgc ggcccccgtc ctgctcgtct cccggctcac
     4621 cctgaccgcc gtcacgacct tcctggccat cgtgctctgg tcccggaccc gggacctcgc
     4681 gtggatgctc atggtggtcg gcacggtggc aggctacgcg gacattctgt acgggctcct
     4741 cttccggttc ggggtggtgc cggaaggccc cctggccgct cccggggttc ccctggccgt
     4801 cctcatcctc tccaaccttc cgaccctcct gtacggagcg gccttcgcgg tcatggtcgc
     4861 ccgcaagcgc ctccgctgaa tccgccgaga acacccctta tagcgcggtt ttcgaaaccg
     4921 cgcgggtcca cgggctcccg cgggagacgc cgccccggcc ggggcaagcg tccggccggt
     4981 tccgggcggt atatccccgg aggtccgcat ggaagtgtac gcgttcgcgc cgaccggatt
     5041 cgagggggga ctggtccggg tggaggtgga tatccgcagg gggctcccgg ccttcgatct
     5101 cacgggcctt cccgacgacg cggtccggga atcccgggac cgcgtccgcg ccgcgatccg
     5161 gaacgcgggg ttcgagttcc ccctggaccg ggtactggtc aacctggccc ccgcggatct
     5221 gcgcaagtcc ggggcctcct tcgacctgcc catagccctg ggcatcctgg cggagagcgg
     5281 ccaggttccg gaggcgggcc ggcccgtcct ggccctgggc gaactcggcc tgggagggga
     5341 tatccggccc gctagggggg ccctcccggc ggtggccgcg gggctttcgg cgggcatcga
     5401 ggacttcgcg gttccccggg agaatctggc ggaagcccgg tccctgggtc ggggcagggt
     5461 gcggggggtc tcctccctgg gggaattctg cgtcctgttt cttccggccg gggccctagc
     5521 ggagggtgca ccctgcccgg aatgcgccgg tactccggcg gatccggatg cggattcccg
     5581 gaccgcggcg tcctggacgg cacaggatgc cggcggcccg gagggggatt tttccgatat
     5641 ccgggggcag ccccagctcc ggcgcgccct ggaggtggcc gccgcgggag ggcaccatgt
     5701 cctgctcttc ggccccccgg gggtcggaaa gaccctggcg gcacgacgcc tcccgaccct
     5761 tcttccggac ctgtccggaa cggccgcggt ggacgtgacc cggatccact ccctcgcggg
     5821 cctcctgggc cccgggaccg gactcctgcg ccgtccgccc ttccggagtc cccaccacgg
     5881 ggcctccgcg gagggggtcg tgggcggagg ccgctgtgcg cggcccgggg agatctccct
     5941 ggcccacggg ggagtcctct tcctggacga ggccccggag ttccgatcgg acatcctcca
     6001 ggccctccgg gagccgaccg aggaggggaa gatatccata gcccgggcgg aggcgaccct
     6061 gtcgttcccg gccaggttcc aactggtcct ggccgccaat ccctgtccct gcggctccct
     6121 gggacggccc ggggccgtct gtctctgttc gatccaggac cttcggcgct actggaaaaa
     6181 gctgggcggc cccctcatgg accggatcga catccgggtc cccgtgaaac cggcgccttt
     6241 ccgggatctc caggcgccgg aggaaccctc ctcggccgtt cgcctcaggg tcgaacgggc
     6301 ccgccggatc caggcggaaa ggttcgaggg cctggattgc gaagtcaacg gccgtatccc
     6361 ggcgggcttc gtggggaggt attgccccgt ggacgctacg actacggcct tcctggaccg
     6421 gcagtccgag gctctcggat tgtcctcccg ggccctgcat tccatcctgc gcgtctcccg
     6481 taccatcgcg gacctggacg gcgccgcttc aatctccctt cgccacgccg aagaggcggc
     6541 ggagctgcgc cgctacgggg acgaggatcc cttctggagg attccctgat gcgtgtttct
     6601 cctctatcca acaagatgga caagaattag ggaaggatcg acgagatgaa tccttcatgc
     6661 gcccagatcc ccgtcttgtt aatcttgaac catcatgtga atcctgttgg atctatggtt
     6721 cc
//
LOCUS       DAOTEI010000188         6574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_8360_length_6574_cov_5.648719, whole genome shotgun sequence.
ACCESSION   DAOTEI010000188 DAOTEI010000000
VERSION     DAOTEI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8360_length_6574_cov_5.648719"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="P5313_14425"
     CDS             complement(<1..601)
                     /locus_tag="P5313_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY81609.1"
                     /translation="MRDSAGLIIYVGKAKILKNRLSSYFSGRKDPKTRLLVSRIASLE
                     WILTETEYEALLLENELIKRNAPKYNINLKDGKSYPVIRITAEEWPRVFRTRRIVDDG
                     SLYFGPFPSAEIIDAYLDLIRRIFPLRRCRRMRKRDTPCMYYHIGRCSAPCAGRIERE
                     AYLAVVEKVRKLLAGETDELLGSLKAQMEEAAADLRFEEA"
     gene            709..2274
                     /locus_tag="P5313_14430"
     CDS             709..2274
                     /locus_tag="P5313_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81610.1"
                     /translation="MTTKRILALILAASAAVCTAAGIETSLRLDASSRPASSPHPWIL
                     SGVAAVPAAGAVPAAGAVPAASAVPAADSGAARVLVLEPARYRWTEGTDLLVHFDAES
                     GWDETGRWRIEPRGSFKHAPADLAYMGSGAASFRAPGSQLVLHPEKSSLFGAGSVSGD
                     FSLEFWLYPANVESGEILFLWKGARRDGTRTTTQQVSAIFSRNRMVFAFANFFAPPGG
                     GAFQLNLTGTSLLVPRTWSHHLVRFDSSTGLVEYLMNGVSEAAAYATATGREGGAVHL
                     PSVGTASPVTLGQNYTGLLDELRLSSSWVERPNLGRYGTEPGSAVSPLVDLGWNNTRL
                     ASVDAEIRTPGDSGAAFFYRVGDFPETWRTDSPEWKPFRPGGSLPESARGRYAQVRVE
                     LYPDPRGADGPILSSITLRYLPDPPPPPPGEVLAAPGDGRVTLRWSRVPEADLAGYLV
                     YYGTSPGDYYGREAVQGASPVDTGNTVSITLTGLANGRLYYFAVASYDAVLPRRPGEF
                     SREVSARPARNVP"
     gene            2271..5405
                     /locus_tag="P5313_14435"
     CDS             2271..5405
                     /locus_tag="P5313_14435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81611.1"
                     /translation="MTAPHPPSDALERARNALRLRDFEAAERLARNYLIQKPESRDAR
                     FVLGTVYLRTNRFEPALGAFAAIVERDAKDVRALENLGAVYLRLGRLDDALAALARGL
                     ELAPDDPELLFNEGSVYRQMGNLKAAEMSFARAVEKDPRHVLALNSLGTVHMARGDHE
                     KAVKVFWKGLAVDQNYPSFHFNLGISYEAMGKTEDALREYEQAVKARPGWTEAMNRYG
                     LALARNGKTRQSTDIFHQVLAVSPRNAEARNGLGVAFAEAGKVDEAIREYRAALEADP
                     GFLKAAENIERLLEQSGRQAEALQEIQGLVEVMPDNLGLRLRLARLYRTLGYNDKSRD
                     AVVDVLRRSPDNLEALKLYGLLLKALGEEGKAEKIFRKVVSLDPKEIGFRIELARIYR
                     EEGRPDDAVKELELFLKARPGNPEARLELARARAARGDRTHAKSILEELTRERPDDAE
                     AWLELSAVYRALGEKEKAVAAADRLVNLRGRRGKPEDIEDLEGSLDLYEKAVGAYSQD
                     VRDAWDRNIQILKENLQAEEGSAAPGEPTPEERMEAVLALASAEPIPVDEAEELIFLE
                     EYEEPLPEEEVPLDSVDLSEEEEEPLGEDERLLAGQELYADSPVGGGGGGEGRGEESS
                     GREGGTPLPEGPSPHAPETRSPEPPPPYTPQAYPPQPYPPPSYPPPSYPPPQQAAPSY
                     APPPQAPAPQAPVPQSPAPAPQPSYAPQASYPPPQPPPRAYPPAPREEPRQRRPEPEP
                     VDEEPMEPMEPPVEAETAPETELPTAEPEPEYEEYDEDLGEEEPEIVFEEGEEPDVEP
                     PERPPLEPEPEPEASPEEVGEPSPDETGPGEPGSADGGSGTEGCASGRPECQGEEIAD
                     LLSYLKGLTDSLPEDKRKELEDSGISRKMDELIQALGDGDLESEDESVRRAAEDKVGR
                     AAAALDGSVPGASSLLKEAQKLGERRGEDRRTGADRRATPKERRTWMDRRDGGDRRRE
                     ARRVREDRRRDERIPASGEAAPSDESDAAPKAETPVVFDPQGRPTEVCGIPVSPKMAK
                     LIEIIRREKGHGRN"
     gene            5392..6261
                     /locus_tag="P5313_14440"
     CDS             5392..6261
                     /locus_tag="P5313_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRY81612.1"
                     /translation="MAGTSNLLKVRDYIGSMPSLPTTVSKVLEVCNNPKTSPIDLDRV
                     IALDPVLMGKVLKLINSAYYGLGTQVTSLVRAIIMLGVNTVKNLALSTAVLDKFTSNK
                     DFRALNMEGFWRHSLCAGVTAKLIAKKRGVDSKSLEEYFAAGLLHDIGKIPLNNALAE
                     EYVRTMAVADRDRVSLHVAEKRTLELDHADAGALIAAAWHLDGAISDAARYHHAFDEY
                     QGENREVVLTVAVANYYASREGIGFSGNLYPDKLPPSVFEELKFRKETLEELEGPVNA
                     EIDKAKVFLRLAQ"
     gene            6299..>6574
                     /locus_tag="P5313_14445"
     CDS             6299..>6574
                     /locus_tag="P5313_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY81613.1"
                     /translation="MFTVKIWGDRGSMPTPGPDTVEFGGNTSCIEVRCGKRLIIIDAG
                     SGIRQLGDSLMRTDFPKGPISADIFITHTHWDHIMGFPMFTPIYVPGT"
BASE COUNT         1076 a         2321 c         2237 g          940 t
ORIGIN      
        1 cggcctcctc gaagcggaga tcggccgcgg cctcctccat ctgggccttc agggatccga
       61 ggagctcgtc ggtctccccg gccaggagct tgcggacctt ctccacgaca gccaggtagg
      121 cctcccgctc gatccggccg gcgcaggggg cggaacagcg gccgatgtgg tagtacatgc
      181 agggggtgtc ccgcttccgc atccgcctgc agcgccgcag ggggaagatc cggcggatca
      241 ggtccaggta ggcgtcgatg atctccgcgc tggggaaggg gccgaagtag aggctgccgt
      301 cgtccacgat ccgccgggta cggaagaccc ggggccactc ctccgcggtg atgcggatga
      361 ccgggtagga tttcccgtcc ttcaggttga tgttgtactt gggggcgttc cgcttgatga
      421 gctcgttttc caggaggaga gcctcgtatt cggtctccgt caggatccac tcgaggctcg
      481 cgatcctgga gaccaggagc cgggtcttcg ggtccttacg gccggaaaag tacgaggaca
      541 ggcggttttt caggattttg gccttgccta cgtatataat tagccccgcg gagtcgcgca
      601 tgaggtatac tcccggggat tccggggcgc ttcgcgcgat tcttcggggt tcctgcgaag
      661 cgggggctat actttgctcc atatcgttcg atactagaat acagagaaat gacgaccaag
      721 agaatactcg ccctgatcct cgcagcctcg gccgcggtat gcaccgccgc cgggatagag
      781 acgtccctgc gcctggacgc ctcctcccgg cccgcctctt caccgcaccc ctggatcctc
      841 tcgggcgtcg ccgcggtccc ggccgcgggt gcggtccctg ccgcaggcgc ggtcccggcc
      901 gcgagtgcgg tccccgccgc ggactccggc gcggcccggg tcctggtcct ggagcccgcc
      961 cgataccggt ggaccgaggg aacggatctc cttgtccact tcgacgccga atccgggtgg
     1021 gacgagaccg gacggtggag gatcgagccc cggggcagct tcaaacacgc ccccgcggac
     1081 ctggcctaca tgggatccgg ggcggcctcc ttccgggccc ccggctccca gctggtcctc
     1141 caccccgaga agagctccct cttcggcgcc ggctccgtgt ccggggactt ctccctggaa
     1201 ttctggctct accccgccaa cgtcgagtcc ggggagatcc tcttcctctg gaagggggcc
     1261 cggcgggacg ggacccggac tacgacccag caggtgtccg cgatcttctc ccggaaccgc
     1321 atggtcttcg cgttcgccaa cttcttcgcc ccccccgggg gaggggcctt ccagctgaac
     1381 ctcaccggaa cctccctcct ggtccctcgg acctggagcc accacctggt gcgcttcgat
     1441 tcctccacgg ggctcgtgga gtacctgatg aacggggtct ccgaagcagc cgcctacgcc
     1501 accgccaccg ggcgggaggg cggggcggtc cacctcccct cggtgggcac ggcgtccccc
     1561 gtgaccctgg gccagaacta cacggggctc ctggacgagc tccgtctctc ctcctcctgg
     1621 gtggagcgtc cgaacctggg aaggtacggg accgagccgg gatctgcggt atcgcccctg
     1681 gtggacctcg gctggaacaa cacccgactc gcctccgtcg acgccgagat ccgcaccccc
     1741 ggggactccg gggccgcctt cttctaccgc gtcggggatt tccccgaaac ctggaggacg
     1801 gacagccccg agtggaaacc cttccggccg ggcgggtccc tgccggaaag cgcccggggg
     1861 cgctacgccc aggtgcgggt ggagctctac ccggaccccc ggggcgccga cgggccgatc
     1921 ctgtcctcga tcaccctacg gtacctgccc gatccccccc cgcccccccc gggggaggtc
     1981 ctggccgcgc ccggggacgg gagggtcacc ctccggtgga gccgggtgcc cgaggcggac
     2041 ctggcggggt acctggtgta ttacggaacc tccccggggg actattacgg cagggaagcc
     2101 gtgcagggag cctccccggt ggacacgggc aatacggtct cgatcaccct gacgggcctc
     2161 gccaacggca ggctctacta tttcgccgtg gcttcctacg acgcggttct cccccggcgg
     2221 ccgggggaat tctcccggga agtatccgcc cgtcccgcca ggaacgtccc gtgacggccc
     2281 cccaccctcc ctcggacgcc ctggagaggg cccggaacgc cctccgcctc cgggacttcg
     2341 aggccgcgga gcgcctggcc cgcaactacc tgatccagaa gcccgagtcc cgggacgccc
     2401 gcttcgtcct cggcacggtc tatctccgca ccaaccgctt cgagcccgcc ctcggggcct
     2461 tcgccgccat cgtggagcgg gacgccaagg acgtccgggc cttggagaac ctgggagccg
     2521 tctacctgcg cctgggccgc ctggacgacg ccctggccgc cctggcccgg gggctcgagc
     2581 tggccccgga cgatccggag ctcctgttca acgagggcag cgtctaccgc cagatgggga
     2641 acctgaaggc cgcggagatg tccttcgccc gggcggtgga gaaggatccc cggcacgtcc
     2701 tggccctgaa ctccctgggc accgtgcaca tggcccgggg ggaccacgag aaggccgtca
     2761 aggtgttctg gaagggcctc gcggtggacc agaactaccc ctccttccac ttcaacctgg
     2821 gcatctccta cgaggccatg ggcaagaccg aggatgccct ccgggagtac gagcaggccg
     2881 tgaaggcccg gccgggctgg acggaggcca tgaaccgcta cggcctggcc ctggcccgga
     2941 acgggaagac ccggcaatcc acggatatct tccaccaggt cctggccgtg tccccccgga
     3001 acgcggaagc ccgaaacggc ctgggcgtcg cgttcgcgga ggccggcaag gtcgacgagg
     3061 ccatccggga gtaccgggcg gccctggagg ccgatccggg cttcctcaag gccgcggaga
     3121 acatcgagcg cctcctggag cagagcggcc gccaggccga ggccctgcag gaaatccagg
     3181 ggctcgtgga ggttatgccc gacaacctgg gcctccggct ccgcctggcc cggctctacc
     3241 gtaccttggg ctacaacgac aagtcccggg acgcggtcgt ggacgtcctc cgccgcagtc
     3301 ccgacaacct ggaagccctc aagctctacg gcctcctgct gaaggccctg ggcgaggagg
     3361 ggaaggcgga gaagatcttc cgcaaggtgg tctccctgga ccccaaggag atcgggttcc
     3421 ggatcgagct ggcccggatc taccgggaag agggccgacc ggacgacgcc gtcaaggagc
     3481 tggagctctt cctgaaggcc cggccgggga atcccgaggc ccgcctggag cttgcccggg
     3541 cccgggccgc ccggggggac cggacccatg ccaagagcat cctggaggag ctgacccggg
     3601 agcgtccgga cgacgcggag gcctggctgg agctctccgc ggtgtaccgg gccctggggg
     3661 agaaggagaa ggcggtggcc gccgcggacc gcctggtgaa cctccgggga cgccggggca
     3721 agcccgagga tatcgaggac ctggaaggct ccctggacct ctacgagaag gccgtcgggg
     3781 cgtattccca ggacgtccgg gacgcatggg accgcaacat tcaaatcctc aaggaaaacc
     3841 tccaggccga ggaggggtcg gcggcccccg gggaacccac cccggaggag cggatggagg
     3901 ccgtccttgc cctggcctcc gcggagccca taccggtgga cgaggcggag gagctcatct
     3961 tcctggagga gtacgaggag cccctgcccg aggaggaggt ccccctggat tccgtggacc
     4021 tctcggagga agaggaggag ccccttggcg aggacgagcg gctcctggcg ggccaggagc
     4081 tctacgccga ttcgcccgtg ggaggcggag ggggcggaga gggccggggc gaggaatcct
     4141 ccggccggga aggcgggact ccgctaccgg aaggtccgtc cccccatgcc ccggaaaccc
     4201 ggagcccgga gccgccccct ccgtacacgc cccaggccta tcctccccag ccctatccgc
     4261 ctccttccta tccgccgccg tcctatcctc ccccccagca ggcggccccg tcctacgcgc
     4321 cgccgcccca ggctccggcc ccccaggccc cggtgcccca gtctccggcg ccggcccccc
     4381 agccgtccta cgcgccgcag gcctcgtatc cgccgcccca gccgcctccc cgtgcctacc
     4441 ccccggcccc ccgggaggaa ccccgccaac ggcgccccga gccggagcct gtggacgagg
     4501 agccgatgga gccgatggag ccgcccgtgg aagcggaaac cgccccggag accgagcttc
     4561 cgacggccga gccggagccg gagtacgagg aatacgacga ggacctcggc gaggaggagc
     4621 ccgagatcgt gttcgaggag ggggaggagc ccgatgtcga gcccccggag cgccctcccc
     4681 tggagccgga acccgagccg gaagcatccc cggaggaggt aggcgaaccg tccccggacg
     4741 aaaccgggcc gggggagccg ggatccgcgg acggcggttc cggaaccgag ggttgcgcct
     4801 ccggccggcc ggaatgccag ggcgaggaaa tcgcggacct cctttcctac ctgaagggcc
     4861 tcacggattc cctcccggag gacaagcgga aggaactgga ggacagcggg atctcccgga
     4921 agatggacga gctgatccag gccctcgggg acggggacct ggagtccgag gacgagtctg
     4981 tccgccgggc cgcggaggac aaggtgggcc gggccgccgc ggccctggac ggctcggtgc
     5041 ccggggcctc gtccctgctc aaggaagccc agaagctggg ggagcgccgc ggggaggacc
     5101 gcaggacggg cgcggaccgg cgcgcgaccc ccaaggaacg caggacctgg atggaccggc
     5161 gggacggggg ggatcggcgc cgggaagcgc gacgggtccg ggaggaccgg cgccgggatg
     5221 aacggatccc ggcttccggc gaggcggcgc cctccgatga atccgacgcc gcgccgaagg
     5281 cggagacccc ggtggtcttc gatccccagg gccggcccac ggaggtctgc gggattccgg
     5341 tttccccgaa gatggcaaaa ttgatcgaaa tcatacgccg ggagaagggt catggcagga
     5401 actagcaacc tcctgaaggt ccgggactac atcggttcca tgccgagcct cccgacgacc
     5461 gtttccaagg tcctggaagt ctgcaacaat cccaagacca gccccatcga cctggaccgc
     5521 gtgatcgccc tggatcccgt gctcatgggc aaggtgctca agctcatcaa ctccgcgtac
     5581 tacggcctgg ggacccaggt gaccagcctg gtccgggcga tcatcatgct gggggtcaac
     5641 acggtgaaga acctggccct ctccacggcg gtgctggaca agttcacctc gaacaaggat
     5701 ttccgggccc ttaacatgga gggcttctgg aggcattctc tctgcgcggg ggtgaccgcc
     5761 aagctgatcg ccaagaagcg gggcgtggat tccaagtccc tggaggagta cttcgccgcg
     5821 gggctcctgc acgacatcgg caagatcccc ctgaacaacg ccctggcgga ggagtacgtc
     5881 cggaccatgg ccgtagcgga ccgggaccgg gtttccctcc acgtggcgga gaagaggacc
     5941 ctggagctgg accacgcgga cgcaggcgcc ctcatcgcgg cggcctggca cctggacggg
     6001 gccatctcgg acgccgcccg gtaccaccat gccttcgacg agtaccaggg ggagaaccgg
     6061 gaggtggtcc tcaccgtggc ggtggccaac tactacgcca gccgggaggg catcggcttc
     6121 tcgggtaacc tctacccgga caagctcccc ccctcggtct tcgaggagct caagttccgc
     6181 aaggagaccc tggaggaact ggagggcccc gtgaacgccg agatcgacaa ggccaaggtc
     6241 ttcctgcgtc tggctcaata gcctccgttc cgttatagtc ttcccaggag acaaacccat
     6301 gttcaccgtc aagatctggg gagaccgggg ctccatgccc acccccggtc cggataccgt
     6361 cgagttcggc ggcaacacct cttgcatcga ggtccgctgc ggcaagcgtc tcatcatcat
     6421 cgacgccggg tccggaatcc gccagctggg cgactccctg atgcgcaccg acttccccaa
     6481 ggggccgatc tccgcggaca tcttcataac ccacacccac tgggaccaca tcatgggctt
     6541 ccccatgttc acgcccatct acgtgcccgg cacg
//
LOCUS       DAOTEI010000189         6556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_8395_length_6556_cov_5.795416, whole genome shotgun sequence.
ACCESSION   DAOTEI010000189 DAOTEI010000000
VERSION     DAOTEI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8395_length_6556_cov_5.795416"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..850)
                     /locus_tag="P5313_14450"
     CDS             complement(56..850)
                     /locus_tag="P5313_14450"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRY81614.1"
                     /translation="MIAAVDARNRTIRVGLGDPGPSGVRWRARFCLGADPLRSAEEYA
                     WTLAGLCARQGVDPLAADRVILSSVVPSLTPRLRSACAQAFGSGEAGLGRVLTVGPGI
                     RTGMKIRTDIPGEVGSDLVCDAVGAYARLGTACIVVDFEAMLTFTAVNGEGELLGVAI
                     APGPQAAAESLRANGAQLPQVRLEPPARAVGKNSAQSIQSGILLGYSGLVDRMVALFR
                     KELDCPAVAAGTGDEIGLALAPEGGYAFFDPWLSLEGLAVLDERNR"
     gene            complement(847..1464)
                     /locus_tag="P5313_14455"
     CDS             complement(847..1464)
                     /locus_tag="P5313_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRY81615.1"
                     /translation="MERSSVRRIVVSGALGAVSVVLAVTPLGYLPWFGGASLTVMHIP
                     AIVAAVLEGPWAGVSVGLIFGATSLVKAAVAPIGPLDPLFTNPLVSVLPRLAIGLAAW
                     AVYAAFRGKFRPVAAALAGAVGSFVNTAGVLTMLGLTAAAQISGVFGVEPGALPAVLA
                     GIAVSNGLVEAAAAAVFTAAIVSAWKGVEGASGKARLAREEGEGT"
     gene            complement(1596..2429)
                     /locus_tag="P5313_14460"
     CDS             complement(1596..2429)
                     /locus_tag="P5313_14460"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRY81616.1"
                     /translation="MKNIEFLRNVSIGMYVEGDSPVHRLSPAAKYLWLLALILPASFS
                     GSAPVVGILAAACLGFALLARIRPRTLLRGFLPVLPLVGLAAFLQVVFTWPQDRSAVL
                     LAAGPVSLTVREVLAVLAMALRFATLMLALGLFTSVITEGDTARGVEDLFAPLERFGF
                     PARGLGLTVAVAFRFIPIVAGELEAIVKAQAARGADLGSRKGGPIRKARAYLPLLVPV
                     TIRALERAEALAEAMEARGWSSGPRTRYAVSGGGRWDVPVKLGALAFAAAALWAGAVF
                     R"
     gene            complement(2426..4249)
                     /locus_tag="P5313_14465"
     CDS             complement(2426..4249)
                     /locus_tag="P5313_14465"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRY81617.1"
                     /translation="MIEIRSLSHTYSGASSPALVDVDLRIEDGEYLAVAGANGSGKST
                     LIRCLNGLVVPPAGAVAVDGLDPSRPEERRSIRRILALVFQNPPDQIVASVVEEDTAF
                     GLENLGVPRAEMVERVRAALEEMGLARESASPTRFLSAGQQQRLAVAGALVLGPKHLA
                     FDEATSMIDPAGRSEILDRMDALAASGATVIHVTHDMDEAARARRVVVLSEGRVAFDG
                     TPEELFRRGNLAALGLAPPRSWSLARALGLPPVVGERPGDLGRRAARALRDGMRPEPL
                     DPEPAIPPGPDRPAGADPERESPPLVSGSEVSAFRADRAGLSYLSGTPQERRALTDID
                     LGIPRGGRVALVGATGSGKSSLLQLLSALAFPQKGRVLSFGIDTSDPKTDLRALRMRT
                     PLSVQRPESALFEYYAGDEVSFGPRNQGLSGSALVARVRSALEAVGLPFDEFRDRPVR
                     SLSGGQKRRLALASVLAMEPEALLLDEPGSALDPVSRERLMGLVYSYGRKGGTVVFAT
                     HSMEEAARADLVVVLAEGRLRAAETPAYIFGAGWDPSWGLARPFGARAAEGAREAGLV
                     LPEGIVDEAGLIRALADLGRGAPSRASAGLPASRTAGEAGA"
     gene            4432..5088
                     /locus_tag="P5313_14470"
     CDS             4432..5088
                     /locus_tag="P5313_14470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY81618.1"
                     /translation="MTCPVCGRREPRNPDAPPWALVRDASYFRCPRCSYVYLDRNLLP
                     TPEEEKARYLLHRNDPEDPGYRSYLEDFIRKAVLPFAPPEGRILDFGSGPVPVLARLL
                     SDRGYRVSAYDPFFAPDRTARTGPFDLIALHEVAEHLARPYFELARIARALAPGGRLA
                     IRTRFAPEDPGDFRTWWYREDPTHVGFFGSRSFAALAGRLGMSVDLDDGRETLVLRSL
                     "
     gene            complement(5095..6429)
                     /locus_tag="P5313_14475"
     CDS             complement(5095..6429)
                     /locus_tag="P5313_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRY81619.1"
                     /translation="MTEARRILRIAAPAMAGSLVETLYNLTDAFFLGKLGSAEISAPS
                     VSTSLVFFLIIFATGLSGAGTTLIAQVKGRGDAERMNFYMNQTAVILAAASGVLTLSG
                     LVLARPILRLLNTPPEVLGPALTYLRIVLGGMPFMFAYFLLQSSFSAIGDTVTPLKVH
                     LTAVLANLVLDPLLIFGFGPIPALSVAGAAVATVLSQGLGAFLSLRILAKGRGPLRLR
                     PALLRPDPKAAALILRIGLPSSVGQALSALGFTVLQGVVNLFGTGAIAAFGVGNRLIG
                     LFDLPAHGLSVATTTLVGQALGARDEDRAARVVGSALRLCLVLVGLPLAASVAFGGHL
                     VRFFVADPEAVAVGDLMFKVVAPSVLFFCLYLVLSGAFQGAGDTRVIMVLSVVRLWIV
                     RVPLAYALAFLAGMGPMSIWIAMFVSNLATALAGLAWFRGGRWRKALGTAEL"
BASE COUNT         1097 a         2346 c         2306 g          807 t
ORIGIN      
        1 acatgatgaa ttggcggtag ccgaccgatg tgaacccggc aaacagctct tccatctacc
       61 tattcctttc atccagaacg gccagcccct ccagggacag ccaaggatcg aagaacgcgt
      121 atcccccctc gggggccaaa gcaaggccga tctcgtcccc cgtgcccgcc gcaaccgcgg
      181 gacaatccaa ctccttgcgg aacagggcca ccatccggtc cacgaggccc gaatatccca
      241 gcaggatccc ggactggatg gactgggcgg agttcttccc gacggcccgg gccggaggct
      301 ccaggcgcac ctggggaagc tgggccccgt tggcccggag gctttccgcc gcggcctggg
      361 ggcccggggc gatggcgacg cccaacagct ccccctcccc gttcacggcg gtaaaggtca
      421 gcatggcctc gaaatccacc acgatgcaag cggtccccag ccgggcgtaa gccccgacgg
      481 cgtcgcagac caggtccgag cctacctccc cgggaatgtc ggtgcggatc ttcatccccg
      541 tccggatccc cggtcccacg gtcaggaccc ggccgagccc cgcctcgccg gacccgaagg
      601 cctgggcgca ggcggaccta agccgcgggg tcagggaggg caccaccgag gagaggatta
      661 cccgatccgc ggctagaggg tctacaccct gccgggcgca gaggcccgcc agggtccatg
      721 cgtactcctc cgcgctccgc aggggatcgg ctcccaggca gaaccgggcc ctccaacgga
      781 ccccggaggg cccgggatcg cccaggccca cccggatcgt ccggttccgg gcatccaccg
      841 cggcgatcat gtcccctcgc cttcctcccg ggccaggcgg gccttgccgg aggctccttc
      901 aacgcccttc caggcgctca cgatggccgc cgtgaagacc gccgcggccg ccgcctcgac
      961 cagtccgttg gacacggcga tccccgccag caccgccggc agggccccgg gctccacgcc
     1021 gaagactccg ctgatctggg ccgccgcggt gagccccagc atggtcaaga ccccggcggt
     1081 gttcacgaag cttccgacag ccccggccag ggccgccgcg acgggccgga acttgccccg
     1141 gaaggcggcg tacacggccc aggccgccag cccgatggcc aggcggggca ggacggacac
     1201 caggggattc gtaaacaggg gatccagggg gccgatgggg gccaccgcgg ccttgaccag
     1261 gctggtcgcc ccgaagatga gccccacgga gacccccgcc cagggccctt ccagcacggc
     1321 ggccacgatg gccgggatgt gcatgaccgt cagggaggcc cctccgaacc agggcaggta
     1381 ccccaggggg gtcaccgcca agaccacgga cacggcgccc agggcgccgg acaccacgat
     1441 acgccgcacg gaagatcgtt ccacgttcca cctcccaagg tcccggtgca cgcccgtggg
     1501 gggtcgcgcc cggaccgcag attctatgga gatcggcgga accgaaatcc gggccgccga
     1561 cctcccgccg gccccctcga gggccgggtc gtgccctatc taaaaaccgc gcccgcccaa
     1621 agggccgcgg cggcgaaagc caaagcgccc agcttgaccg ggacgtccca gcggccgccc
     1681 ccggatacgg cgtagcgggt cctgggaccc gaggaccagc cccgggcctc catggcctcc
     1741 gccagggctt ccgcccgctc cagggcccgg atcgtcacgg gcaccagcag gggcaggtag
     1801 gcccgggcct tccggatcgg tccgcccttc cgggatccca ggtcggctcc ccgggcggcc
     1861 tgggccttca cgatggcctc cagctccccc gcgacgatgg ggatgaaccg gaaggccacc
     1921 gccaccgtca ggcctaggcc ccgggcggga aagccgaacc gctccagggg agcgaatagg
     1981 tcctccaccc cccgggccgt gtccccctcg gttatcaccg acgtgaagag ccccagggcc
     2041 agcatcaggg tggcgaaccg gagcgccatg gccagcaccg ccaggacctc ccggaccgtg
     2101 agggagacgg ggcccgccgc caggaggacg gcgctccggt cctggggcca ggtgaatacc
     2161 acctgcagga acgcggccag gcccaccagg gggaggaccg gcaggaagcc ccggagcagg
     2221 gtccgggggc ggatccgggc gaggagggcg aatcccaggc aggcggccgc caggatcccc
     2281 acgaccgggg cggatccgga aaagctggcc ggcaggatga gggccaggag ccagaggtac
     2341 ttggccgcgg gggagagccg gtggaccggg gaatccccct ccacgtacat cccgatggag
     2401 acgttccgga ggaactcgat gttcttcatg cccccgcctc cccggcagtt cgagacgccg
     2461 gaagccccgc ggatgcacgg gacggcgcgc ccctgccgag atccgcaagg gcccggatca
     2521 ggcccgcctc gtccacgatc ccctcgggca ggaccaggcc cgcctcccgg gctccctccg
     2581 cggctcgggc gccgaagggc cgggcgaggc cccaggaggg atcccacccg gctccgaaga
     2641 tatacgccgg ggtttccgcg gcccggagcc ggccttcggc caggacaacg accaggtccg
     2701 cccgggccgc ctcctccatg gaatgggtgg cgaacaccac ggtcccgccc ttccttccgt
     2761 aggagtagac cagtcccatg agccgttccc gggataccgg gtccagggcg gaccccggtt
     2821 cgtccaggag cagggcctcg ggctccatgg ccaggaccga ggccagggcc agccgccgct
     2881 tctgtccgcc cgacagggat cgtacgggcc ggtcccggaa ctcgtcgaag ggcaggccca
     2941 cggcctccag ggcggaacgg acccgggcca ccagggcgga ccccgagagc ccctgattgc
     3001 ggggcccgaa agaaacctcg tcccccgcgt agtactcgaa gagggccgat tccggacgct
     3061 ggacggaaag gggggtacgc atccggaggg cccggaggtc cgtcttcgga tccgaggtgt
     3121 cgatcccgaa ggaaaggacc ctgcccttct gggggaaagc cagggcggaa aggagctgga
     3181 gcaggctgga ttttccggaa cccgtggccc ccaccagggc gacccggccg ccccggggta
     3241 ttcccaggtc gatgtccgtc aaggctcggc gctcctgggg ggtgcccgaa agatagctga
     3301 ggcccgcccg gtccgcccgg aaggcggaaa cctcggaacc ggagaccaag gggggcgact
     3361 cccgttccgg atccgccccg gcgggccggt cgggcccggg cggaatggcg ggttccgggt
     3421 ccagcggttc gggacgcatc ccgtcccgga gggcccgggc cgcccggcgc cccaggtccc
     3481 cgggcctctc tcccaccacg ggcggaaggc ccagagcccg ggccaggctc caggaccgtg
     3541 ggggggccag gcccagggcg gccaggttcc cccgccggaa gagctcctcg ggggttccgt
     3601 cgaaagccac ccgtccctca gacaggacca cgacccgtcg ggcccgggcg gcctcgtcca
     3661 tgtcgtgggt gacgtggatg accgtggctc ccgaggccgc cagggcgtcc atccggtcca
     3721 ggatctccga ccgccccgcg ggatcgatca tggaggtagc ctcgtcgaag gccaggtgct
     3781 taggtcccag gaccagggcg ccggcgacgg ccaggcgctg ctgctggccc gcggacagga
     3841 agcgggtcgg gctggcgctc tcccgggcca gccccatctc ctccagggcg gcccggaccc
     3901 tctccaccat ttccgcccgg gggacgccga ggttctcgag gccgaaggcg gtgtcctcct
     3961 caacgacgga ggctacgatc tggtccgggg gattctggaa gaccagggcc aggatccgcc
     4021 ggatggagcg gcgttcctcc gggcgggacg gatccaaccc gtccaccgcg acggcgcccg
     4081 caggagggac gaccaggccg ttgaggcagc ggatcagggt actcttaccg gagccgttcg
     4141 ccccggccac ggccaggtat tccccgtctt ctatccgaag gtccacgtcg accaacgcgg
     4201 gcgaggaggc gccggaatag gtgtgggaaa gggatcgtat ctcgatcaac tcgggcccgc
     4261 cggagggcgc gggatactca cgaggaggga aggcatagac acctcgcgtc tcggtcccga
     4321 cggccgtacg gaaggttccg gggcttcgtc gatgcggatc caagcgccgt ggtcaaggta
     4381 cgcgttcccg ggggatttgt caagaaaacc ctcgtccgct agagtggggg catgacctgt
     4441 cccgtctgcg gccgccggga accccggaac cccgatgccc cgccctgggc cctcgtgcgg
     4501 gacgcctcgt atttccggtg cccccggtgc tcctacgtat acctagaccg gaacctcctg
     4561 cccacgccgg aggaggagaa ggcccgttac ctcctgcacc ggaacgatcc cgaggatccc
     4621 ggctaccgct cctacctgga ggatttcatc cggaaggcgg tcctgccctt cgccccgccg
     4681 gagggcagga tcctggactt cgggtccggg cccgtgcccg tcctggcccg cctgctctcg
     4741 gaccgggggt accgggtctc cgcgtacgat cccttcttcg ccccggaccg cacggctcgg
     4801 accggcccct tcgacctgat cgccctccac gaggtggccg agcacctggc ccgcccgtac
     4861 ttcgagctcg cccggatcgc ccgggccctg gcccccgggg gccgtctggc gatccggacg
     4921 cgcttcgctc cggaggatcc cggggacttc cggacctggt ggtaccggga ggatcccacc
     4981 cacgtgggat tcttcgggag ccgctccttc gcggccctgg ccggacggct ggggatgtcg
     5041 gtggatctgg acgacggccg ggagaccctg gtgctacgaa gcttgtgaag ggattcagag
     5101 ctccgcggtc cccagggcct tcctccatcg gccgccccgg aaccaggcga gtcccgccag
     5161 ggccgtggcc aggttggaca cgaacatggc tatccagatg gacatgggtc ccatcccggc
     5221 caggaaggcc agggcgtagg ccaggggtac ccggactatc cagagccgga cgacggacag
     5281 gaccatgatg acccgggtgt ccccggcacc ctggaaggcc ccggacagca ccaggtagag
     5341 gcagaagaag aggaccgagg gcgccacgac cttgaacatg aggtccccga cggccaccgc
     5401 ctccgggtcg gccacgaaaa accgcaccag gtgcccgccg aacgcgaccg acgccgccaa
     5461 gggcaggccc accaggacca gacagaggcg aagggcggat cctacgaccc gggccgcccg
     5521 gtcctcgtcc cgggccccca gggcctggcc gaccagggtg gtggtcgcca cggacagacc
     5581 gtgggcggga aggtcgaaga ggccgatcag tcggttcccg accccgaagg cggcgatggc
     5641 cccggtcccg aagaggttca ccaccccctg gaggaccgtg aagcccaggg cggacagggc
     5701 ctggccgacc gaggaaggca ggccgatgcg gaggatcagg gccgcggcct tgggatccgg
     5761 ccggaggagg gcgggccgca ggcgcagggg cccccttccc ttcgccagga tgcgcaggga
     5821 gaggaaggcc cccaggccct gggagaggac cgtcgcgact gcggcgccgg ctaccgacag
     5881 ggccgggatc ggaccgaagc cgaagatgag caggggatcc aaaaccaggt tggccaggac
     5941 ggcggtcagg tggaccttca ggggggtcac ggtgtccccg atcgcggaaa aggacgattg
     6001 gagcaggaag taggcgaaca tgaagggcat gccgcccagt acgatgcgca ggtaggtcag
     6061 ggccggtccg aggacctccg ggggggtgtt gagcaggcgg agtatagggc gggcgagaac
     6121 caggcccgag agggtcagga ccccggacgc ggcggcgagg atgaccgcgg tctggttcat
     6181 gtagaagttc atccgctccg cgtcgccccg gcccttcacc tgggcgatca gggtcgtccc
     6241 ggcgcccgaa agccccgtgg cgaagatgat caggaagaac accaggctgg tcgacacgga
     6301 gggcgcggag atctccgcgg aacccagctt tcccaggaag aaggcgtccg tcaggttgta
     6361 gagggtctcc accaggctgc ccgccatcgc gggggccgcg atcctgagga tcctcctggc
     6421 ttccgtcatg ccgccagagt agccgcccgg gcccggcatg tccagggagc ccgggttcga
     6481 ttgacgagta ttccccttcc ggttagactc ggcgaagtat gaagatttgg ctgaaactgc
     6541 tcatcgggac cctgct
//
LOCUS       DAOTEI010000190         6536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_8429_length_6536_cov_5.412282, whole genome shotgun sequence.
ACCESSION   DAOTEI010000190 DAOTEI010000000
VERSION     DAOTEI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8429_length_6536_cov_5.412282"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="P5313_14480"
     CDS             complement(<1..352)
                     /locus_tag="P5313_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81620.1"
                     /translation="MRNTRAKAALELTGKLIERFGPRLAGSEASLGCADALLAEARGA
                     ADKAWAEDFRVHPGAFLGFIRVLVVLYLVSAPALFFAPALSAVLMTAGIGILIFGFFL
                     YREVLEINLPWLERV"
     gene            complement(463..1845)
                     /locus_tag="P5313_14485"
     CDS             complement(463..1845)
                     /locus_tag="P5313_14485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY81621.1"
                     /translation="MSGTFPRWGAVCAVLLSLLVLGCAQVVEPKASPEESAVSVDELL
                     ADLDALGAVQSKALFGASSIPKPTGGYFTADELSGYLSGIKDALASCAAAAASNSPYL
                     VMPVKLKTGGQSGLMWVPVSLGRTLSVPIIAYQHGTRVYWENAPSRFNSNPLSVLSSR
                     DVSGAFQNYVECVAGALMASAGYIVVMPDYPGFGDSRAPHPYVHLSLGESVRVLVGRA
                     RTVLATLWGAARANNRLYLIGYSEGGFATMAAAKRMQETGMHVTAAVPCAGSYDLSGT
                     MVREVLSGEEAPVPYYIPYTVFGYASVYRNVEPAAWDYAALLKPPLPGILDTLFGGAY
                     SGAEVSAAMPSKVLRELITDTLAAELAAGTGTVFGRLLKNTAFRTGWIPGMILQMIHS
                     PEDGIVPVENTLAAYEAFKFLPNVLPPIFVPPVPLPEALVNLADVHLFAYPTALLAGF
                     TFIDGVESRF"
     gene            2106..2795
                     /locus_tag="P5313_14490"
     CDS             2106..2795
                     /locus_tag="P5313_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRY81622.1"
                     /translation="MKTIGLIGGMSWESSGVYYTLINREVRRRLGKSHSASCVMWSFD
                     FQEIEELQYAGDWEALRKRMFQAGRGLAAAGADFAVLCTNTMHKVMEGFEAAAGIPYL
                     HIVDAAGEALAADGPGTVGLLGTKFTMEGGFYADRLRDRFGLTVLVPEAEDRAAVNDV
                     IYGELVRGEIREESRTRYAEVIDRLADRGARGIVLGCTEIGLLIREHPRVPLYDTTVL
                     HARAAVDRALG"
     gene            complement(2814..3245)
                     /locus_tag="P5313_14495"
     CDS             complement(2814..3245)
                     /locus_tag="P5313_14495"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRY81623.1"
                     /translation="MKDLVHDTIELEFRFDAAPERVFEALTRGIGAWWSHAFREGSKV
                     GLDPRPGGAFGESWSGGGAVYAVVRHLDPPETLILDGPMGMDGAVAASMEYTLDPDGT
                     GTRLRLVHDILGRIDPDTIADYRAGWEAVLGISLRDYLAGK"
     gene            complement(3273..3818)
                     /locus_tag="P5313_14500"
     CDS             complement(3273..3818)
                     /locus_tag="P5313_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81624.1"
                     /translation="MKAINRLVAALLLALSLCAPVLAQSADAPFTDRELAKLIADWPS
                     VVQWMEAKGRQLDAAESAGPGAFAAMMAGADLEAFLKGKGWTLARFSYVAGTAFMLAA
                     YVTFERQNPDMIKQFDEAIAQIRANPSLSPADKAQAIQGLEEAKKSVLALPEEAKVNE
                     AELKLVRARYDALYKVMDESN"
     gene            complement(3898..4305)
                     /locus_tag="P5313_14505"
     CDS             complement(3898..4305)
                     /locus_tag="P5313_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81625.1"
                     /translation="MKSSKTLPLIVLVALSAFPAVSQTQDAPFTDWELARFIHDWYPV
                     MEWIHLGGGKFDYENPERSAAELFQGSEFEARLKRRFYWTSERFRYMAGSVFRLCLLV
                     EADDPGGARDPQEYELVKTRLGEIAEARRKAGD"
     gene            complement(4483..4911)
                     /locus_tag="P5313_14510"
     CDS             complement(4483..4911)
                     /locus_tag="P5313_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRY81626.1"
                     /translation="MIIPDLNVLLYAVDAGSARHERAEAWLKEEVNGGTEEIGLPWAV
                     ILGFLRLTTSSRVFPRPLSIEQALEWIRDLAARPSVRILDPGKAHLGILGHLLLASGT
                     GGNLTTDAHLAALCLEQDAVIVTGDRDFQRFPGLRVELLY"
     gene            complement(4908..5132)
                     /locus_tag="P5313_14515"
     CDS             complement(4908..5132)
                     /locus_tag="P5313_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81627.1"
                     /translation="MRELQDRAARDSTTLKDEVNRTLAKGLGRDPVGRSPWKPVSYRM
                     GTPAADLDKAWEVADSLEADAAIAKRDLRK"
     gene            5349..6167
                     /locus_tag="P5313_14520"
     CDS             5349..6167
                     /locus_tag="P5313_14520"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRY81628.1"
                     /translation="MAKRHRIDPSDYRSIVFLTGAGISVASGIRPYRGPGGLWNDETL
                     VKFSDLETFRKDPLAVWRFMSETRRISAAARPNPAHLALAELEVRLEAEQSLTVITQN
                     IDGLHRRAGSSHVIEFHGSVTRTRCSNHRCDFPAFEDPLLHADSVPLCSVCGAPLRPD
                     VVFFGEIIPSVRGAAAMEALADCDLFVAVGTSASVYPATRFVEWAWKRNARTVFVNVE
                     SFRDRDPVAATWFREEYIGPAERILPRLFGEGLDEEELDGYRPEVLEDLPEARE"
     gene            6202..>6536
                     /locus_tag="P5313_14525"
     CDS             6202..>6536
                     /locus_tag="P5313_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY81629.1"
                     /translation="MEKTDLSFMFLKKGLAAGRDLDLVLRVETPARTSEGARKPADLA
                     LVLDRSGSMQGRKIVYARQGAAAAVGLLGQEDRASLVQFDDRVDLLQPLTPAARSGAL
                     QAAIAGIEPR"
BASE COUNT         1128 a         2168 c         2204 g         1036 t
ORIGIN      
        1 ccacgcgctc gagccagggc agattgattt ccagcacttc ccggtacagg aagaacccga
       61 agatcaggat gccgatgccc gctgtcatga ggaccgccga gagggcgggg gcgaagaaga
      121 gggccggggc ggagaccagg tacaggacca cgagcacccg gatgaagccc aggaaggccc
      181 ccgggtggac ccggaagtcc tcggcccagg ccttgtcggc cgcgccccgc gcctccgcca
      241 ggagggcgtc cgcacagccc agggacgcct cgctcccggc cagccgcggc ccgaagcgtt
      301 cgatgagctt tcccgtcagt tccagcgcgg ccttcgccct cgtattacgc atagtcatcc
      361 tccccgcatc agtatgcacg ggagggaatc aaaacggaag actgttcggg atccgaacct
      421 cggggagggg cggcgcccgc gcccctcccc gggggggttc gatcagaagc ggctctccac
      481 tccgtcgatg aaggtgaacc ctgcaagcag ggccgtcggg tacgcgaaga ggtgcacgtc
      541 cgcgagattc accaaagcct ccggcagggg cacgggaggc acgaaaatag gcggaagcac
      601 gttcggcagg aacttgaagg cctcgtacgc cgccagggtg ttctccacgg gcacgatgcc
      661 gtcctcgggg gaatggatca tctgcaggat catgccggga atccatcccg tccggaacgc
      721 cgtgttttta agcagccgcc cgaatacggt gccggtgccg gccgcgagtt cagccgccag
      781 ggtgtccgtg atgagttccc gtagcacttt ggagggcatc gccgcggaca cctccgcccc
      841 ggagtacgcg ccgccgaaca gggtgtccag gattcccggg aggggcggct tcaatagggc
      901 ggcgtaatcc caggctgcgg gctccacgtt ccggtagacc gaggcgtagc cgaagaccgt
      961 ataggggatg tagtagggaa ccggcgcctc ctccccggag aggacttccc ggaccatcgt
     1021 acccgaaagg tcgtaggagc ccgcgcaggg caccgcggcg gtcacgtgca tgcccgtttc
     1081 ctgcatgcgc ttggccgcgg ccatcgtggc gaatccgccc tcggaatagc cgatgagata
     1141 caaccggttg ttggcgcggg ccgctcccca gagggtggcg agcaccgtcc gggcccggcc
     1201 gaccaggacg cggacggact cgcccaggga aaggtggaca taggggtgcg gagcccggct
     1261 gtcgccgaat cccggataat cgggcatcac cacgatgtac cccgccgagg ccatcagggc
     1321 gccggccacg cactccacgt agttctggaa agcccccgag acgtcccgac tcgagaggac
     1381 ggacaggggg ttcgagttga accgggacgg ggcgttctcc cagtacaccc gcgtcccgtg
     1441 ctggtaggcg atgatcggga ccgagagggt cctgcccagg ctgacgggca cccacatgag
     1501 cccgctctgt cccccggttt tcaacttgac gggcatcacc aggtacggac tgttcgaggc
     1561 ggccgcggcc gcacaggaag ccagggcgtc cttgatgccc gagaggtacc ccgacaactc
     1621 gtcggcggtg aaatacccgc ccgtcggttt gggtatggac gaggcgccga acagcgcctt
     1681 gctttgtacg gcccccaggg cgtcgagatc ggcaaggagc tcgtccacgg aaaccgcgga
     1741 ctcctccggg gacgccttgg gttccacgac ctgggcgcat cccaggacca gcagggacag
     1801 gaggacagcg cagactgccc cccagcgggg aaacgttcca ctcatgattt cctccccggc
     1861 gcggcgcgtg cggccgcgcc gatacagcga acccgccgcc gaagccgggt agcggttcgg
     1921 cggtccatcg tcgctaggat gtcgtttggg tggtacacta tcgggcggcg gccgagtcga
     1981 tgccggggaa ggttccggac gccgaacggt tcgggggcac cggcgattca agcatagtgc
     2041 cggcccgccg gattcgcaag cgccgggaac cccggaggcc gaccaagatc gcttggaggg
     2101 acggcatgaa gaccatcgga ctgatcggag gcatgagctg ggaatcctcg ggggtctact
     2161 acaccctgat caaccgggag gtgcggcggc ggctgggaaa aagccattcc gcgtcctgcg
     2221 tgatgtggtc cttcgacttc caggagatcg aggagctcca gtacgccggg gactgggagg
     2281 ccctgcgcaa gcgcatgttc caggcgggca ggggcctcgc ggccgctggg gcggacttcg
     2341 cggtcctgtg cacgaacacg atgcacaagg tcatggaggg tttcgaggcc gccgcgggca
     2401 tcccctacct gcacatcgtg gacgccgcag gggaggccct ggccgcggac ggcccgggca
     2461 cggtcggcct cctgggcacg aagttcacca tggagggagg cttctacgcg gaccggctcc
     2521 gggaccgctt cggccttacg gtcctggtac cggaggcgga ggaccgggca gcggtgaacg
     2581 acgtcatcta cggggagctc gtccggggtg agatccggga ggagtcccgg actcgctacg
     2641 ccgaggtcat cgaccgcctg gccgaccggg gggcccgggg gatcgtgctg ggctgtacgg
     2701 agatcggcct cctgatccgg gagcaccccc gggtgccgct ctacgacacc acggtcctcc
     2761 acgcccgggc cgcggtggat cgggcgctgg ggtaggagga ttctccgcct accctacttc
     2821 cccgccagat aatcccgaag gctgatcccc agcacggctt cccagcccgc acggtagtcg
     2881 gcaatcgtgt ccggatcgat ccggcccagg atgtcgtgga ccaggcggag ccgggtgccg
     2941 gttccgtcgg gatccagggt gtactccatg gaggcggcga ccgccccgtc catgcccatg
     3001 gggccgtcca ggatcagggt ctccggcgga tcgaggtggc ggaccacggc gtagacggcg
     3061 cccccgccgg accaggactc cccgaaggcg ccgcccgggc gcggatccag gccgactttg
     3121 gagccctccc ggaaggcgtg ggaccaccag gcgccgatgc cccgggtgag ggcctcgaag
     3181 acccgctccg gtgcggcgtc gaaccggaat tcaagctcga tcgtgtcgtg gactaggtct
     3241 ttcatggaac ctccggcgcc ttcgccccgg gcctagttcg actcgtccat caccttgtag
     3301 agggcgtcat accgggcccg gaccagcttc agctcggcct cgttcacttt cgcctcctca
     3361 ggcagggcca ggacgctctt cttggcttcc tcgagaccct ggatcgcctg ggccttgtcc
     3421 gcgggggaca gggacggatt cgcccggatc tgggctatgg cctcgtcgaa ctgcttgatc
     3481 atatccggat tctgacgctc gaaggtgacg taggcggcca gcatgaaggc cgtgcccgca
     3541 acgtagctga accgcgccag ggtccagccc ttgcccttca ggaaggcttc caggtccgcc
     3601 cccgccatca tcgcggcgaa ggcgccgggc cccgcggact cggcggcgtc cagctggcgg
     3661 cccttggcct ccatccactg gaccaccgag ggccagtcgg cgatgagctt ggccagctcc
     3721 cggtccgtga agggggcgtc ggcggactgc gccaggacgg gggcgcagag ggacagggcg
     3781 aggaggaggg cggcgacaag ccggttgatt gctttcatgg gagaaagtat agcacgctcc
     3841 gtcaaggacg gaaggcagga cgcggaccga tggctccggc agtccacggg agtgtatcta
     3901 atcccccgcc ttcctgcggg cttccgcgat ctcgcccagg cgcgtcttca cgagctcgta
     3961 ctcctgtgga tcccgggctc ctcccggatc gtccgcttcc acaaggaggc ataaccggaa
     4021 gaccgaaccc gccatgtacc ggaaccgctc cgaagtccag tagaatcgcc gcttgagtcg
     4081 agcctcgaac tcggatccct ggaacaattc cgcagcggag cgctccgggt tctcgtaatc
     4141 gaactttcct cctccaagat gaatccactc cattacgggg taccagtcgt ggataaatcg
     4201 tgcgagttcc cagtcggtga agggggcgtc ctgggtctgg gatacggcgg gaaaggcgga
     4261 cagggctacg agaactatta aagggagtgt ttttgagctt ttcataccaa gattctagac
     4321 ccgctcgcct cgcttgtaaa acgactttta tgaaaatcta gaccttggaa cgcgcccact
     4381 tccacaagcc ccaggcggcc atgcccagga gcacgacgta ctgggccgag acgaagcgga
     4441 cgtccaccgg ataacgccga aagagggcta ggcccgggga gctcagtaaa gcagttcgac
     4501 ccggaggccc ggaaaccgtt ggaaatcccg atctcccgtg acgatcaccg cgtcctgttc
     4561 cagacacagg gcggccaggt gggcgtccgt cgtgaggttg ccccccgtac ccgaggcgag
     4621 caggaggtgc cccagtatcc cgagatgggc ctttccggga tccaggatac ggaccgacgg
     4681 ccgggccgcc aggtcccgga tccactccag ggcctgttca atggataaag gccgcgggaa
     4741 gacacggctc gaggtcgtga gccggaggaa cccgaggatc acggcccagg gaagtccgat
     4801 ctcctccgtt cccccgttca cctcctcctt cagccacgcc tccgcccgct cgtggcgggc
     4861 cgagcccgcg tcgacggcgt acagcaggac gttcaggtcc gggatgatca cttgcgcagg
     4921 tcccgcttgg cgatcgcggc gtcggcctcc aggctatccg cgacctccca ggccttgtcg
     4981 aggtccgcgg cgggagtccc catccggtag gaaacgggct tccacgggga tcggccgacc
     5041 gggtcgcgcc ccaggccctt tgccagggta cggttcacct cgtccttgag ggtcgtcgag
     5101 tcccgggccg cccggtcctg tagctcgcgc atcaatatgt ccgaaatatc gatcgtagtg
     5161 cgcatatgga catcatactg ctatatcccg ccggggtcaa tatttcgggt ccgaatccgc
     5221 ccacacgagt ctttaaccgg ccgcaaacga tttttctgat ttttcattga cgtcgcgtac
     5281 atagtgtagt atatacacta ttagatgacc cgcggccgat cctcgtgacc ggcttcgagg
     5341 aggaaaccat ggcaaaacgg catcgaatcg atccttcgga ctacaggtcc atcgtcttcc
     5401 tgaccggggc ggggatatcc gtggcctcgg ggatccgccc ctatcgggga cccgggggcc
     5461 tgtggaacga cgagaccctg gtcaagttct cggacctcga gactttccgg aaggatcccc
     5521 tagcggtctg gcgcttcatg agcgagaccc ggaggatctc cgccgccgcc cggcccaacc
     5581 ccgcccacct cgccctggcg gagctggagg tacggctgga ggcggagcag tccctgacgg
     5641 tcatcaccca gaacatcgac ggcctccacc gccgggccgg gagctcccac gtgatcgagt
     5701 tccacggctc cgtgacccgg acccggtgct ccaaccaccg ctgcgacttt ccggccttcg
     5761 aggatcccct cctgcacgcg gattccgtgc ccctctgctc ggtctgcggc gcccccctcc
     5821 ggccggacgt ggtcttcttc ggggagatca tcccctccgt ccggggcgcc gcggccatgg
     5881 aggccctggc ggactgcgac ctcttcgtag ccgtcggcac ctcggcctcg gtataccccg
     5941 cgacccgctt cgtggaatgg gcctggaagc ggaacgcccg gacggtgttc gtgaacgtgg
     6001 agtccttccg ggatcgggat cccgtggccg ccacctggtt ccgggaggag tacatcgggc
     6061 ccgcggaacg gatcctgccc cggctcttcg gggagggcct ggacgaggag gagctggacg
     6121 ggtaccggcc cgaggtactg gaggatctcc cggaagcccg ggagtagcgg gaattctggc
     6181 acgaacgcga ggagaagaat catggagaaa acggacttga gtttcatgtt cctgaaaaag
     6241 ggcctggccg ccggccggga cctggatctc gtgctccggg tggagacccc ggcccgtacc
     6301 tccgagggcg cacgcaagcc cgcggacctg gccctggtcc tggaccgttc cgggtccatg
     6361 cagggccgca agatcgtcta cgcccggcag ggtgcggccg ccgccgtggg cctcctgggg
     6421 caggaggacc gggccagcct ggtgcagttc gacgaccggg tggacctcct ccagcccctg
     6481 acccctgccg cccggtccgg cgccctccag gccgccatcg cgggcatcga gccccg
//
LOCUS       DAOTEI010000191         6424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_8627_length_6424_cov_5.742032, whole genome shotgun sequence.
ACCESSION   DAOTEI010000191 DAOTEI010000000
VERSION     DAOTEI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8627_length_6424_cov_5.742032"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="P5313_14530"
     CDS             complement(<1..285)
                     /locus_tag="P5313_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81630.1"
                     /translation="MVRRTSIIIIAIACLAAVGAALYLGLAFYGNSPSAYGRVLTQYL
                     KALSAGDEAAALALTADGFVNELSEVKLVPGNFRAYDFGFQGPATSDPATL"
     gene            complement(356..1090)
                     /locus_tag="P5313_14535"
     CDS             complement(356..1090)
                     /locus_tag="P5313_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HRY81631.1"
                     /translation="MRIAIDTHTHSVASGHAYSTVDELARGVRDRGLEGFVLTDHGPA
                     LPGGAHPYHFGNIRILPRIIEGVRFYRGIEANILDLEGRIDLEPYWIGYLDFVMAGFH
                     EACFTNRGRDENTRALIAALENPLVDAISHPGNPAFPIEIPAVVARAAALGKALEVND
                     SSFRVRKGSSDTCLTLARACAQADCPVVCGSDAHYWADAGRLDTAVDTLDRAGVPEAL
                     VVNARTDRFEAFVARRRAEKGFLQAS"
     gene            1379..2524
                     /locus_tag="P5313_14540"
     CDS             1379..2524
                     /locus_tag="P5313_14540"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRY81632.1"
                     /translation="MASPSLPPSTFEAAFGADPELASAARVAVSSALRLQPGEKVLIV
                     ANPCRDSIEISRALFDAVLDAEARPSLLVQPVKAQLDYAEETLIAAFESHPDVVMSIS
                     AQKMGKDRSGLETPYEWDEVSYDHVFHYQLYGAKTLRAFWSPRITRDSFLRTVPIDYS
                     ELRGRCRRIKEVLDKAERIRVTNPKGTDITIGARGRSAFSDDGDFSQPGQGGNLPAGE
                     VFLSPELGTADGVIVFDGSISCVSGDLVLAKPVRAVVRGGFVAEVEGDSEAAEVRRSL
                     EDGAESARRLASEGRLSPEKGEQYARNARNLGELGIGLNPRARITGNMLEDEKVLRTC
                     HFAIGSNYDEDAPALVHFDGLVTEPTITAYLPHGREVVIERDGELLL"
     gene            2554..4131
                     /gene="lysS"
                     /locus_tag="P5313_14545"
     CDS             2554..4131
                     /gene="lysS"
                     /locus_tag="P5313_14545"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRY81633.1"
                     /translation="MEPTKTAHWADMYAQKIIREKGDKETYTCASGITPSGTVHIGNF
                     REIISTELVVRALRRLGKKVRFIYSWDDYDVFRKVPKNMPNQEVLTKHLRFPINLVPD
                     PYERDSSYARHHEVNVEKILPAVGIFPEFLYQAERYRSSMYAEGMRRALERRAEIKAT
                     LDEFRAEDLSGDWWPVSIFCDSCNRDDTTLQSWDGEWKISYTCADCGHAETIDLRSAK
                     GAKLFWRIDWPMRWAREGVDFEPAGKDHHSQGGSFDTARRIIQVVYDAEAPVSFQYDF
                     ISIKGRGGKISSSSGEVIDLYDVLEVYTPEVTRYLFAGTRPNTEFAISFDLDVIKIYE
                     DYDKTERVYYGAETLADPDEVEREKRIYELSQPGEPEPVMPYQVVGGFRHLCNLLQID
                     GGDIDRTLSKLPGLTKAQEPKLRVRAQCAWNWITKFSPEDFRFRLREGGSEIETKPNE
                     KAALAALRDAVCSGGLDEAALAERIYAIAQENGLEPKDFFKTAYRALLGKERGPRLAG
                     FLLGIGPERVKAILAPY"
     gene            4404..5330
                     /gene="cysK"
                     /locus_tag="P5313_14550"
     CDS             4404..5330
                     /gene="cysK"
                     /locus_tag="P5313_14550"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRY81634.1"
                     /translation="MRYAESILDLVGNTPLVKIRAEGAAEGARVLAKLEFFNPFSSVK
                     DRIGLAMIEDAEKRGALKKGSVIVEPTSGNTGIALAAAAAAKGYRIILTMPETMSVER
                     RKLLAAYGAELVLTEGAKGMKGAIAKAREILESTPGAFMPMQFDNPANPEVHRKTTAE
                     EIWRDTDGAVDILVAGVGTGGTISGVSSVIKRRKPSFKAVAVEPADSPVLSGGAPGPH
                     KLQGIGAGFVPANYDPAVVDEVIRVRAEDAGTEARRIAKEEGILVGISSGAALWAARE
                     VARRPESAGKTIVVIFPSSGERYLSTWLFGEM"
     gene            5804..>6424
                     /gene="rsgA"
                     /locus_tag="P5313_14555"
     CDS             5804..>6424
                     /gene="rsgA"
                     /locus_tag="P5313_14555"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HRY81635.1"
                     /translation="MHLDREEILKAYGWTEGFAEAAESPEFEGLEPARVLETRRGAWT
                     VLAAGASGPEELEAAAAGRLLHEAAAQADLPATGDWVLVRPTDPGRALIQAVLPRRSK
                     FSRKRPGETERDRIDEQVIAANVDWAFIVGAAGKDWNPRRAERYLTLAWESGAQPVLV
                     ITKADLAEDLETLLAQAVEAAPGAPVVALCAREGWGLEGLGPYLASW"
BASE COUNT         1101 a         2216 c         2114 g          993 t
ORIGIN      
        1 cagggtcgcc gggtcggaag tcgccggacc ctggaacccg aaatcgtagg cccggaagtt
       61 gccgggcacc agtttcacct cggacagctc gttgacgaac ccgtcggcgg tcagtgcgag
      121 ggcggccgcc tcgtcccccg cggaaagggc tttcaagtac tgggtcagga cccggccgta
      181 ggcggacggg gaattcccgt agaaggccag gccgaggtac agggccgcgc cgacggcggc
      241 cagacaggcg atcgcgatga tgattatgga tgttcgccga accatggaaa gagcctcctg
      301 gagcgccttg gtcccgccga atctactatc cggccggggg agcgtcaacc tggtgttacg
      361 aagcttgtag gaagcctttt tccgccctcc gccgggcgac gaaggcctcg aatcggtccg
      421 tccgggcatt cacgaccagg gcttcgggaa ccccggcccg gtccagggtg tccaccgcgg
      481 tgtccagccg gcccgcgtcg gcccagtagt gggcgtcgct cccgcagacc accgggcagt
      541 ccgcctgggc gcaggcccgg gcgagggtca ggcacgtgtc cgaggagccc ttccggaccc
      601 ggaaggagga gtcgttgacc tccagggcct tgcccagggc agcggcccgt gccacaaccg
      661 ccgggatttc gatcgggaag gccgggttgc cgggatggga gatcgcgtcc accaggggat
      721 tctccagggc cgcgatcagg gcccgggtgt tctcgtcccg gcctcggttg gtgaagcagg
      781 cctcgtggaa gcccgccatc acgaaatcca ggtacccgat ccagtaaggc tccaggtcga
      841 tccgtccctc gaggtccagg atgttcgcct cgataccccg gtagaaccgg actccctcga
      901 tgatccgggg caggatccgg atgttcccga aatggtaggg atgggctccc ccgggcaggg
      961 cgggaccgtg gtcggtcagg acgaagccct cgagcccgcg gtcccggacc ccccgggcca
     1021 gctcgtcgac ggtggagtag gcgtgcccgg acgcgacgga atgggtgtga gtgtctatgg
     1081 ctatgcgcat ggatgaagac agtatccacg aaacgggccg gagtgaccag cctgtcgtgg
     1141 acgtttcgtc cccggcacgc tcatgatttt aaaaatgcga gacaggatgg acaggatagg
     1201 atccaggatg aacaggatga agagagaaag ggaaagacca aaagagagag cacttcaaat
     1261 ttttccatgc atcctgtcca tcctttcttt atcctgttca tcctgtccaa tttctccggt
     1321 aaccgtttct ttttcccgtg ccttgacagc gcctccgggg ccgggattac atgggctcat
     1381 ggcaagccca tccctgcccc cctccacctt cgaggcggcc ttcggcgccg acccggagct
     1441 cgcgtcggcc gcccgggtcg cggtctcctc ggccctcagg ctccagcccg gggagaaggt
     1501 cctgatcgtg gccaatccct gccgggactc catcgagata tcccgggccc tcttcgacgc
     1561 tgtcctggac gccgaggcgc ggccgagcct cctcgtgcag ccggtcaagg cccagctgga
     1621 ctacgcggag gagaccctga tcgcggcctt cgagtcccac cccgacgtgg tcatgtccat
     1681 cagcgcccag aagatgggca aggaccgcag cggcctggag accccctacg aatgggacga
     1741 ggtctcctac gaccacgtct tccactacca gctctacggg gccaagaccc tccgggcctt
     1801 ctggtccccc cggatcaccc gggactcctt cctgcgcacg gttcccatcg actattcgga
     1861 actccggggc cgctgccgcc gcatcaagga ggtcctggac aaggccgagc ggatccgggt
     1921 caccaacccc aagggcacgg acatcacgat cggagcccgg gggcggtccg ccttctccga
     1981 cgacggggac ttctcccagc cgggccaggg gggcaacctg cccgccgggg aggtcttcct
     2041 ctccccggag ctgggcaccg cggacggcgt gatcgtcttc gacggctcca tctcctgcgt
     2101 ctccggggac ctcgtcctgg ccaagcccgt gcgggcggtg gtgcggggag gcttcgtggc
     2161 cgaggtggag ggggattccg aggccgccga ggtccggcgc tccctggagg acggggccga
     2221 aagcgcccgc cgcctggcct ccgagggccg gctctccccg gaaaaggggg agcagtacgc
     2281 ccggaacgcc cgcaacctgg gggagctggg gatcggtctc aacccccggg cccgtatcac
     2341 gggcaacatg ctggaggacg agaaggtcct gcgcacctgc cacttcgcca tcggctccaa
     2401 ctacgacgag gacgccccgg ccctggtcca cttcgacggc ctcgtgacgg agcccacgat
     2461 cacggcctac ctgccccatg gccgcgaggt cgtgatcgag cgggacgggg agctcctatt
     2521 gtaggacccg gtcccgttct gtatactccg gccatggaac cgaccaagac cgcccactgg
     2581 gcggacatgt atgcccagaa gatcatccgg gaaaagggtg acaaggaaac ctacacctgc
     2641 gccagcggca tcaccccctc gggtacggtg cacatcggca acttccggga gatcatctcc
     2701 acggaactcg tggtccgggc cctccgccgc ctgggcaaga aggtccgctt catctacagc
     2761 tgggacgact acgacgtctt ccgcaaggtc cccaagaaca tgcccaacca ggaggtgctg
     2821 accaagcacc tgcggttccc gataaacctc gtgccggatc cctacgagcg ggactccagc
     2881 tacgcccgcc accacgaggt gaacgtggag aagatcctgc ccgcggtggg gatcttcccc
     2941 gagttcctct accaggccga gcgctaccgg tcctcgatgt acgccgaggg gatgcgccgg
     3001 gccctggagc ggcgcgccga gatcaaggcg accctggacg agttccgggc cgaggacctc
     3061 tccggggact ggtggccggt ctccatattc tgcgactcct gcaaccggga cgataccacc
     3121 ctccagtcct gggacgggga gtggaagatc tcctacacct gcgccgactg cggccacgcg
     3181 gagaccatcg acctccgcag cgccaagggg gccaagctct tctggcgcat cgactggccc
     3241 atgcgctggg cccgggaagg cgtggacttc gagcccgcgg gcaaggacca ccactcccag
     3301 ggcggatcct tcgacaccgc ccggcggatc atccaggtgg tgtacgacgc ggaggccccg
     3361 gtctccttcc agtacgactt catctccatc aaggggcggg ggggcaagat ctcctcctcc
     3421 tccggggagg tgatcgacct ctacgacgtc ctggaggtct acacccccga ggtgacccgt
     3481 tacctcttcg cgggcacccg tccgaacacc gagttcgcca tctccttcga cctggacgtc
     3541 atcaagatct acgaggacta cgacaagacc gagcgggtct actacggggc ggagaccctg
     3601 gccgaccccg acgaggtgga acgcgagaaa cggatctacg agctctccca gcccggcgag
     3661 cccgaaccgg tcatgcccta ccaggtcgtg ggcgggttcc gccacctctg caacctactg
     3721 cagatcgacg ggggggacat cgaccggacc ctgtccaagc tgccgggact cacgaaggcc
     3781 caggagccca agctccgggt ccgggcccag tgcgcctgga actggatcac caagttctcc
     3841 cccgaggact tccgcttccg cctccgcgag ggcggctcgg agatcgagac caagccgaac
     3901 gagaaggccg ccctggcggc cctgcgggac gcggtctgct ccggaggctt ggacgaggcg
     3961 gccctggcgg aacggatcta cgcgatcgcc caggagaacg gcctcgaacc caaggacttc
     4021 ttcaagacgg cctaccgggc cctcctgggc aaggagcggg gcccccggct tgcgggcttc
     4081 ctcctgggga tcggcccgga gcgggtgaaa gccattctcg cgccgtattg atcggcgcga
     4141 ggatggcccg tcagaatcga cgaggccgat aggcttcgtc gccgccatgc cgactcctgt
     4201 cggcatggcg cgacttattg acgtcgcccg ggttccagga gtcaacgttc tctgtcctcg
     4261 atcctgcctc actccgtgat ctccgtgtcc tccgtggtga gttcgggaag cctttcgccc
     4321 ggcgcggccg gcgccggaat ttgtccggcg ccgcggttcc tccgtatatt tataattcgg
     4381 gtttcggccc ggacggaggg atcatgcggt acgcggagag cattctcgat ctcgtgggga
     4441 acacccccct tgtaaaaata cgggccgagg gcgcggccga gggcgcccgc gtcctggcca
     4501 agctcgaatt cttcaacccc ttttccagcg tcaaggaccg gataggactc gcgatgatcg
     4561 aggacgcaga aaaaagagga gccctcaaga agggctcggt catcgtggaa cccacctccg
     4621 gcaacaccgg catcgccctg gccgccgcgg ccgcggccaa gggctaccgc atcatcctga
     4681 ccatgccgga gacgatgagc gtcgagcgcc gaaaactcct ggcggcctac ggggccgagc
     4741 tcgtcctgac cgagggcgcc aagggcatga agggcgccat cgccaaggcc cgggaaatcc
     4801 tggaatccac ccccggggcc ttcatgccca tgcagttcga caaccccgcg aacccggagg
     4861 tccatcgaaa aaccaccgcc gaggagatct ggcgggacac ggacggagcg gtggacatcc
     4921 tggttgcggg ggtcggcacg ggaggcacga tctccggagt ctcctcggtg atcaagcggc
     4981 gcaagccctc cttcaaggcc gtggccgtgg agcccgcgga ctccccggtc ctttccggag
     5041 gcgccccggg cccccacaag ctgcagggga tcggcgcggg cttcgtgccc gcgaactacg
     5101 atccggccgt agtggacgag gtgatccggg tacgggccga ggacgcgggg acggaagccc
     5161 ggcggatcgc caaggaagaa ggcatcctgg tgggcatctc ctcgggcgcc gccctgtggg
     5221 cggcccggga ggtggcccgc cggccggaaa gcgcgggcaa gaccatcgtg gtcatcttcc
     5281 cgtcctcggg ggagcgctac ctgtcgacgt ggctgttcgg ggaaatgtga ataagtgaat
     5341 cgagaatggg tgaatcgtgc ggaacttctt attcacccat tcacccattc acccattcac
     5401 ccattcaccc attcacccat tcaccttcca ccctttacac tcccctctcg actccgctat
     5461 actccctccc ggtcgcgcgg acctgaccgc gcccgccggg acgcctggcg tcccctatcc
     5521 cgatccgccg ggggtttctg tggaaaaggc ccttgtactc gccgccgagg ccgcgctccg
     5581 gggggaccga ggataaacgc cgaaccgcgc ccggacgccc gagcgtctgt gtagcgcccg
     5641 ttctccgaac gctcccgctc ccaacccctc cgttcccccc tcgcgccccc ggataggatt
     5701 atggacccag gctcgccccg gcacgcgccg aggccgagtg gtaccggacg ccccgcgccg
     5761 aaggcccggg cgccgtccga gctcgagagg gaatacaccc atcatgcatc tggatcgcga
     5821 agagatactc aaggcatacg gctggaccga gggattcgcc gaggcggccg agtccccgga
     5881 attcgagggg ctggagcccg cccgggtcct ggagacccgg cgcggggcct ggacggttct
     5941 ggcggccggg gcctccggcc cggaggagtt ggaggcggcc gccgccggcc gccttctcca
     6001 cgaggccgcg gcccaggcgg acctgcccgc cacgggggac tgggtcctgg tccgcccgac
     6061 ggatcccggc cgtgcgctta tccaggccgt cctgccccgc aggtcgaagt tctcccggaa
     6121 acgccccgga gagaccgagc gggaccggat cgacgagcag gtgatcgccg ccaacgtgga
     6181 ctgggccttc atcgtcggag ccgcgggaaa ggactggaat ccccgccggg ccgagcgcta
     6241 cctgaccctg gcctgggagt ccggcgccca acccgtcctg gtgatcacca aggcggacct
     6301 ggcggaggac ctggagaccc tgctcgccca ggccgtggaa gccgccccgg gggctcccgt
     6361 ggtcgcgctc tgcgcccggg agggctgggg cctggagggc ctcggaccct acttggcctc
     6421 gtgg
//
LOCUS       DAOTEI010000192         6406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_8658_length_6406_cov_3.960636, whole genome shotgun sequence.
ACCESSION   DAOTEI010000192 DAOTEI010000000
VERSION     DAOTEI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8658_length_6406_cov_3.960636"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="P5313_14560"
     CDS             <1..399
                     /locus_tag="P5313_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRY81636.1"
                     /translation="QVGANMDQNERVFIGTMTAQALGLQGAQGSAEQIAIDDPTKANA
                     AIGTVDAALRVVSKQRADLGAYQNRFEMAAKGVAIAAENLQAAESRIRDTDMASQMVD
                     YVKNQILSQAGGAMLAQANTRSQSVMQILA"
     gene            534..863
                     /locus_tag="P5313_14565"
     CDS             534..863
                     /locus_tag="P5313_14565"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HRY81637.1"
                     /translation="MDVSVIQHGLPQAEILREAAAREAAARTAPSPAPQEPVNMELVL
                     SELQQVSQAFNRRLSFALNEKLDQVVVKVIDSETDKIIKEIPPAELQHVHERIREVIG
                     ILFDERA"
     gene            929..2935
                     /gene="fliD"
                     /locus_tag="P5313_14570"
     CDS             929..2935
                     /gene="fliD"
                     /locus_tag="P5313_14570"
                     /inference="COORDINATES: protein motif:HMM:NF005188.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRY81638.1"
                     /translation="MSDITIPGVTSKYDTKKLIEDLMKVERIPRDRAEEQLKALQLQK
                     TAWQDVNRSIVRARETSRALFSFQNPFNERIAKSSDEGVLTAAATREALEDEHTIVVK
                     QTASADRFLSGRLASDYRVPAGDYAFTVGDRAVSLKFSGGTLKEFADALTRKGGDILR
                     ARVIPVSKSEQSIVIESLKTGSKNRLGFEGAARDWALASGLVEEARSSRRDLATGSAQ
                     PFEKPLDRSLVRAEPGSLAVGPGGEAKLPVSPSLSTKGLVLEMEVRVTRRTEPPAPAG
                     PPPGPSISAPGSITYEGITITADPSDPQVPAWTPPPPPTRTDDPVVLFLSDGAGRSVP
                     LPAQPDSEEFRTVSVTLSDYLADFSGLGIRNRNTHRDVEVRNVRVYDPTETGGLRPRD
                     PVDTSRDAIVSLEGIEVVRDTNSISDLIPGVTLNLQEASDKKIRLKVEPDREKSKEAI
                     IEMVGNYNRLMADINILSRNDERLVQEISYYTEEERKTATARLGLLQGDSTLNLLRTS
                     MQRITTNPQPVGETSEVLGRIGISTNAARGGAGGYDASRLRGYLEIDEEALGKALRDD
                     FETVRRLFGYDTDGDLLVDSGVAFGLDALYRPYVETGGILAVKTRTFDTQISSQQTRI
                     ETLDRQLVAKEAELRRKYGMMEGALQGMERSSQDISNFMKQNDN"
     gene            3035..3907
                     /locus_tag="P5313_14575"
     CDS             3035..3907
                     /locus_tag="P5313_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81639.1"
                     /translation="MTITVNGNEFDMDTAGASLGEVLASADDILERAGSVILGLRLDG
                     TEIGADDLPGLRDKPASEYRLLEIDSAPASELRGRAYGSLLEFLGVLREWSAGTGPSE
                     PLREAWETYGRTYSGLLSSDEHSFLDLLGRDLESADVGGEGRRRLARHADSLEPIFRE
                     RLEELRDPAAALRSASRLYEAERQNLMDVSVRLQTGKDAQAMRSILLFVEIFNKANRI
                     LPELARIGMDTSSLRIDGNSIPEFYESFNGILRQLMEAFEAKDAVLIGDLAEYEIVPR
                     MNAFFETMTGALVP"
     gene            3904..4998
                     /locus_tag="P5313_14580"
     CDS             3904..4998
                     /locus_tag="P5313_14580"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY81640.1"
                     /translation="MSVYLPVLQPYWKQEDPTQNIVVFGVLGALILFAVVSSLLSRGK
                     AGSRRSSLTGGLPSFSRGAFRKAAAEAGLSDADAHFLEEFARASRASNPEFVFKNPGR
                     LDSFFKDALRHIDKTAEAEASAEERRSNLLRIRESLEVRRTLGPQVSSTRQLRPRTTL
                     SFIASDETNYPSVVVAVEAGGLAVEPPPDALGRPVHFRRGTKLTVYFYGTGHQGYNFR
                     TRVRGYEELRGKLVLVLKHSDSITPLPSRKHQRRDLREACTFRRVRVEVRKERGKTLK
                     DVFVENLPFPGTAVDISAGGISVQTANPLDAQEYVKIDFDVGEGAFSAFGIVVRTNRL
                     RGGGVMHVRWVKISRKALNSVLSYVYGYTD"
     gene            5071..5331
                     /locus_tag="P5313_14585"
     CDS             5071..5331
                     /locus_tag="P5313_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81641.1"
                     /translation="MRIVDLLNISRKETPLFYRRVYTAQAVFESPRGRAEKDVQFTVE
                     HLPVGGVEIQVDRIENLDYPLIPVVRELKTYIAALDKKGSLP"
     gene            5337..5768
                     /gene="tsaE"
                     /locus_tag="P5313_14590"
     CDS             5337..5768
                     /gene="tsaE"
                     /locus_tag="P5313_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRY81642.1"
                     /translation="MRTRSPRETRDLGELIGRSVPPGTVIAFRGGLGAGKTTLAKGIA
                     RGLGVEDEVTSPTYTLVFEYRGRMPLRHVDAYRLSSPAEFEEIGASELMGTDGLCLIE
                     WSENVAAALPAEAVVVEILPGDGPEARIVEISGSPLEELLP"
     gene            5765..>6406
                     /gene="tsaB"
                     /locus_tag="P5313_14595"
     CDS             5765..>6406
                     /gene="tsaB"
                     /locus_tag="P5313_14595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRY81643.1"
                     /translation="MNVLGLDCSADTLCAAVRRSGSPSGSPSGDGSAAQGKPGIPKHP
                     FREPRGHETRDGTIVLEIAAGLRHAQRILPAVDLCLREAGLELRDLDLLACAAGPGSF
                     TGLRIALSTVKGLSSALGIPFVTVPTPDCLAAEWEGACPVVIPVLDAQRGRFYAAVYE
                     RGRRTSGYLDLPLTELLRYADAFPEVLFTGPAADLLVGSEEGRPGLRIVRYGRG"
BASE COUNT         1129 a         2201 c         2064 g         1012 t
ORIGIN      
        1 caggtgggcg cgaacatgga ccagaacgag cgcgtcttca tcggcaccat gaccgcccag
       61 gctctcggac ttcagggagc ccaggggtcg gcggaacaga tcgccatcga cgacccgacc
      121 aaggccaacg ccgcgatcgg aaccgtggat gccgctctgc gcgtcgtgtc caagcagcgg
      181 gccgacctgg gcgcctacca gaaccgcttc gagatggccg ccaagggcgt cgccatcgcc
      241 gcggagaacc tgcaggccgc cgagagccgg atccgggaca cggacatggc ctcccagatg
      301 gtggactacg tcaagaacca gatcctgagc caggccggcg gagccatgct cgcccaggcc
      361 aacacgcgct cccagtcggt gatgcagatc ctagcctagg aaaaccgtta ttgcgggctt
      421 agcgtcgaag agatttctgg acgtcttctt tttgccgcgg cccgcggcag acccaggacg
      481 ggaggggatc gcgcccctct cccgttcttt tcttcccgtt cgaggaggaa gacatggatg
      541 tttccgttat acagcacggc ttgccccagg cggagatcct gagggaagcc gcggcgcggg
      601 aagccgcggc acggaccgcc ccctcccccg ccccccagga gccggtcaac atggagctgg
      661 tcttgagcga gctccagcag gtcagccagg ccttcaaccg ccgcctctcc ttcgccctga
      721 acgaaaagct cgaccaggtg gtggtcaagg tgatcgattc ggagaccgat aagatcatca
      781 aggagatccc gcccgcagag ctccagcacg tccacgagag aatccgcgag gtgatcggaa
      841 tcctgttcga cgagcgggcg tgacgaaccc gccggagggg ctcggagcct cttcggcatc
      901 ttgatcggta aggaaggcct cgtccgccat gtcggacatt acgatccccg gcgtcaccag
      961 caagtacgac accaagaaac tcatcgaaga cctgatgaag gtcgaacgca taccccggga
     1021 ccgcgccgag gagcagctga aggcccttca gctgcagaag acggcctggc aggacgtcaa
     1081 ccgtagtata gtccgggccc gggagacctc ccgagccctg ttctccttcc agaacccctt
     1141 caacgagcgt atcgccaagt cctcggacga gggcgtcctg acggccgcgg ccacccggga
     1201 agccctggag gacgagcaca ccatcgtcgt caagcagacc gcctccgcgg accgattcct
     1261 ctccggccgc ctggcctcgg actacagggt accggcgggc gactacgcct tcaccgtggg
     1321 cgaccgggcc gtcagcctta aattctcggg cgggaccctc aaggagttcg ccgacgccct
     1381 cacccgcaag ggcggggaca tcctccgcgc ccgggtgatc ccggtatcca agtccgaaca
     1441 gtccatcgta atcgagtccc tgaagacggg ctccaagaat cgcctgggat tcgaaggcgc
     1501 ggcccgcgac tgggccctgg cctcgggcct ggtggaggaa gcccgctcga gccgccggga
     1561 tctggccacc gggtcggccc agcccttcga gaaacccctg gatcgcagcc tggtgcgggc
     1621 cgagccgggc tccctggcgg tcggcccggg cggggaggcc aagcttcccg tctccccctc
     1681 cctttcaacg aagggtctcg tgctggagat ggaggtacgg gtgacccgcc ggacggagcc
     1741 tcccgccccg gcgggccctc ccccgggacc ctccatttcg gccccgggct ccatcaccta
     1801 cgaggggatc acgatcaccg cggatccctc ggatccccag gtgcccgcct ggaccccccc
     1861 gccgcctccg acgcggacgg acgatccggt cgtgctcttc ctgtccgacg gggcgggccg
     1921 atcggtgccc ctccccgccc agccggattc cgaggagttc cgcacggttt cggtgacctt
     1981 gtcggactac ctggcggact tttccggtct tgggatccgc aaccgcaaca cccataggga
     2041 cgtggaagtc cgtaacgtcc gggtctacga tcccacggaa accgggggcc tgcggcctcg
     2101 ggatcccgtg gacacctccc gggacgcgat cgtctccctg gaggggatcg aggtggtccg
     2161 ggacaccaat tccatctccg acctgatccc cggggtgacc ctgaacctcc aggaggcttc
     2221 ggacaagaag atacgcctca aggtggagcc ggaccgggaa aaatccaagg aagccatcat
     2281 cgagatggtg gggaactata accgcctgat ggcggacatc aacatcctct cccggaacga
     2341 cgagcgcctg gtacaggaaa tctcgtacta caccgaggag gagcggaaga ccgccacggc
     2401 gcgcctgggt ctcctgcagg gcgattccac cctgaacctg ctccggacct ccatgcagag
     2461 gatcaccacg aatccccagc cggtcgggga gaccagcgag gtcctgggcc gcatcgggat
     2521 atccacgaac gccgcccgag gcggagcggg aggctacgac gcgtcccgcc tccggggtta
     2581 cctggagatc gacgaggaag ccctcgggaa ggccctcagg gacgacttcg agaccgtccg
     2641 ccggctgttc ggatacgaca cggacgggga cctgcttgtg gactccggcg tcgccttcgg
     2701 actggacgcc ctctaccggc cctacgtcga gaccgggggc atactggccg tgaagacccg
     2761 gaccttcgat acccagattt ccagccaaca gacccggatc gagaccctgg accgccagct
     2821 cgtggccaag gaggcggagc tccggcggaa gtacggcatg atggaaggag ccctccaggg
     2881 catggagcgc tcttcccagg atatcagcaa ttttatgaaa cagaacgata attagaagtg
     2941 tgaataggtg aatgggtgaa tacgtgaata gatcgctgca agtatcctat tcacctattc
     3001 acatattcac ccattcacct cctcaggaga cggcatgacg attacggtga acggcaacga
     3061 gttcgacatg gataccgccg gggccagcct gggcgaggta ctcgcgagcg cggacgacat
     3121 cctggaacgg gcggggtccg tgatcctggg tctccgcctg gacggaacgg agatcggagc
     3181 cgacgacctg cccggtctcc gggacaagcc cgcctcggaa taccggctcc tggagatcga
     3241 cagcgccccc gcctccgagc tgcgcggccg ggcgtacggg agcctcctgg agttcctggg
     3301 cgtcctccgg gaatggtccg ccggcacggg accgtcggaa cccctccggg aggcctggga
     3361 gacctacgga cgcacatact ccggcctgct ctcctcggac gagcattcct tcctggacct
     3421 cctgggacgg gacctggaat ccgccgatgt cggcggagag gggcgccggc gtctggcccg
     3481 gcacgcggac agcctggagc cgatcttccg ggagcgcctg gaggaactcc gggatcccgc
     3541 ggccgccctg cgatccgcct cccggctcta cgaggcggag agacagaacc tcatggacgt
     3601 ctccgtgcgc ctccagaccg gcaaggacgc ccaggccatg cgcagcatcc tgcttttcgt
     3661 ggaaatcttc aacaaggcca accgcatcct tccggagctg gcccggatcg gaatggacac
     3721 ctcgagcctc cggatcgacg gtaattccat cccggagttc tacgagtcct tcaacggcat
     3781 cctgcgccag ctcatggaag ccttcgaggc gaaggacgcc gtgctgatcg gcgacctggc
     3841 ggagtacgag atcgtgcccc ggatgaacgc cttcttcgag acgatgacgg gagccctggt
     3901 cccatgagcg tctaccttcc ggtcctgcag ccctattgga agcaggagga tccgacccag
     3961 aacatcgtcg tcttcggagt cctcggggcc ctgatcctgt tcgccgtcgt ctcctccctg
     4021 ctgagccggg gcaaggcggg ttcccggaga tcctccctca ccggagggct tccctccttt
     4081 tcccgggggg ccttccgcaa ggccgccgcg gaggccgggc tctcggacgc ggacgcgcat
     4141 ttcctggagg agttcgcccg ggcttcccgg gcctccaatc ccgaattcgt gttcaagaat
     4201 cccggccgcc tggactcctt cttcaaggac gccctccgac atatcgacaa gaccgcggag
     4261 gcggaggctt ccgcggagga acgccgctct aacctgctcc ggatccggga gtccctggag
     4321 gtccgccgca ccctgggccc ccaggtcagc tcgacccggc agctccggcc ccgtacgacc
     4381 ctgtccttca tagcctccga cgagaccaac tacccttccg tggtggtggc cgtggaggcg
     4441 ggaggcttgg ccgtggagcc cccgcccgac gccctggggc ggcccgtcca tttccgcagg
     4501 ggcaccaagc tgacggtcta cttctacggc accggacacc agggatacaa cttccgcacc
     4561 cgggtccggg gatacgagga actccggggc aagctggtcc tcgtgctcaa gcactcggac
     4621 tcgatcaccc ccctgccctc ccgcaagcac cagcgtaggg acctccggga agcctgcacc
     4681 ttccggcggg tgcgggtgga ggtccggaag gaacggggca agaccctgaa ggacgtcttc
     4741 gtggagaacc tccccttccc gggcaccgcg gtcgacatct ccgccggcgg aatctccgtg
     4801 cagaccgcga atcccctgga cgcccaggaa tacgtcaaga tcgatttcga cgtcggggag
     4861 ggggccttct cggccttcgg gatcgtggtc cggaccaacc gtctccgggg cggcggagtc
     4921 atgcacgtcc gctgggtcaa gatctcccgg aaggccctga actcggtgct gtcctacgtg
     4981 tacgggtaca cggactaggg ttcagcgctc ggtcccgggc cggggcccaa ccttgacggg
     5041 cccctcggcg gtgccgtaca cttttcgatc atgcgaatcg tggatctgct gaacatctcc
     5101 cgcaaggaga ctcccctctt ctaccgccgc gtctacacgg cccaggccgt cttcgaatcc
     5161 ccccgggggc gggcggagaa ggacgtgcag ttcacggtcg agcacctccc cgtgggcggg
     5221 gtggaaatcc aggtggaccg gatcgagaac ctggactacc cgctcatacc cgtggttcgg
     5281 gaactcaaga cctatatcgc ggccctggac aagaagggtt ccctgccctg aagcgagtgc
     5341 gaacccgttc cccccgggaa acccgggacc tcggcgaact gatcggccgg tccgtgcccc
     5401 cggggactgt catagccttc cggggaggtc tgggggcggg caagaccacc ctggccaagg
     5461 gcatagcccg ggggctgggg gtcgaggacg aggtcaccag ccccacgtac accctggtgt
     5521 tcgaatatcg gggccggatg cccctgcgcc acgtggacgc ctaccgcctg tcctcgccgg
     5581 cggagttcga ggagatcggg gcctcggaac tgatggggac ggacggcctt tgcctgatcg
     5641 aatggagcga gaacgtggcc gccgccctgc ccgccgaggc cgtggtcgtg gagatccttc
     5701 cgggtgacgg gcccgaggcg cgcatcgtgg aaatctcggg ttcacccctg gaggaactcc
     5761 tgccatgaac gtcctgggac tggattgctc cgccgacacc ctgtgcgccg ccgtacgccg
     5821 ttccggcagt ccttcgggca gcccctcggg cgatggttcg gccgcccagg gcaagccggg
     5881 aattcccaaa catcccttcc gagagccccg gggacacgag acccgggacg gcacgatcgt
     5941 cctggaaatc gccgcgggcc tccgtcacgc ccagaggatc ctgcccgccg tcgacctctg
     6001 cctgcgggag gcgggccttg aactacggga cctggatctc ctggcctgcg ccgcggggcc
     6061 cgggtccttc acggggctgc ggatcgccct atccacggtc aaggggcttt cgtccgcact
     6121 gggtatcccc ttcgtaacgg tgcccacccc ggactgtctg gccgcggaat gggaaggggc
     6181 ctgtcccgtg gtcatccccg tcctggacgc ccaaaggggg cgattctacg cggccgtcta
     6241 cgagcgagga cggaggacct caggctacct ggacctgccg ctcaccgagc tgttgcggta
     6301 cgcggacgcc tttccggagg tcctcttcac ggggcccgcg gcggacctcc tggtcggttc
     6361 cgaggaaggc cggccgggtc tcaggatcgt ccgctacggc cgagga
//
LOCUS       DAOTEI010000193         6386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_8691_length_6386_cov_4.496446, whole genome shotgun sequence.
ACCESSION   DAOTEI010000193 DAOTEI010000000
VERSION     DAOTEI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8691_length_6386_cov_4.496446"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..688
                     /locus_tag="P5313_14600"
     CDS             230..688
                     /locus_tag="P5313_14600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY81644.1"
                     /translation="MMETRDYEQLALESGVYKDIELDILIDTLDSWKARPGSPYSLVE
                     VRDSREPAGFCLFQRAANTESTFDIHTFLVGRDYRGKGAAEKLLDLLAEEILASVRSA
                     MIRIETSSTKENAVEPRFFTARGFETIGHIPDFYGPGNDYCIYAKQVART"
     gene            827..2068
                     /locus_tag="P5313_14605"
     CDS             827..2068
                     /locus_tag="P5313_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY81645.1"
                     /translation="MRTTVEAYSLRNAAALVQESSTKRGNLSVQSGKISAGKAAGPEI
                     DLGGSSFLYPALINIHDHFRGNYVPQVGPNGNAFYINWSPWDNDLKASPVYKERANIT
                     VEQMYFLSSYKNLFSGVATANDHFPHEINEPFLPRLPIRVIGEYTLAHECSSFDLKWG
                     DGIEIEHKRAVERNWPFITHLEEGFDPESQDGIGILERLGCLDGHDVFIHCIGFSDED
                     IAKTAKAGASISWCPASNMLMFNVTCKIRKFLEAGVNVCLGTDSTHTGSANLLSEMKF
                     ARKVFRDMYGTELPAKTLYRMVTANPAKAFRMQDRTGSLDTGKSADLLVLKAIHDDPY
                     EALAKAEPSDIRLLTLEGVPLLVEEALAEPFRELLAKGYETVKVGGRKVHVRGTPASL
                     YRDVRKAIGFDKKLDYLPFEP"
     gene            2164..3231
                     /locus_tag="P5313_14610"
     CDS             2164..3231
                     /locus_tag="P5313_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY81646.1"
                     /translation="MPKAANYRANSVIYFQGDVISDKIYILQSGQVVVNYTDIETGKD
                     LREVIQTGEFFGVKSALGKYPREENAVVVSDAQVLIFTVPEFEQFVSANTRIILKMLR
                     VFSNQLRRIHKQVENLMEKSEALSPEIGLFRTGEYYLKNRMYSQAKYVFSRYLTYYPA
                     GKLADAASKNLETSESCMVKFGDGKGPSPSVAGPPAGTLSAHPGAGAAPAKPADTLRP
                     QAGQALSDTAKEYYNAVSLFSQEKYKEALGEFKRIVEANQDPEYVVKAAFDMGRSVFM
                     LGQFDAAIAHFTRMVQTYPKHPDLADILYYLGQSYAKKGDVERAKGFFRKILTMEKDE
                     DNSTFLRAKKALRGLEEAQNG"
     gene            3224..3871
                     /locus_tag="P5313_14615"
     CDS             3224..3871
                     /locus_tag="P5313_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY81647.1"
                     /translation="MADLSAFTRFARTYQAGEIIFSEYEPGDSFYLIQSGKVRIAKIV
                     GDIEKTIDVLQPPEIFGEMAILEGTPRSATAVAVDTVRVLEFNRANFEVLMMGNPQIA
                     LRLLKLFTKRIYDQKRRFMILTLEDPQARVADVFLMLDETQAAATAGAEQDGEKREFK
                     VTIEDVAHWGGMGASEVQTILGHFANQRRVELYPDRVVVRNISDFSRFVNSRRKK"
     gene            complement(4137..5570)
                     /locus_tag="P5313_14620"
     CDS             complement(4137..5570)
                     /locus_tag="P5313_14620"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRY81648.1"
                     /translation="MKNVSIGAKAFVVSALILAGLMVAAGILTRVVPAGTYERIEGPS
                     GPTLVPGSYRVTERPDYPAWRWLTAPAEVLGSPDALTALVIIAFIAVAGGSFAGLNEA
                     RVLEAAVAVLARRYRTRRFRLLAAVSLFGMLLGSLMGVFEESVLLVPVAAALAASFGW
                     DVLTGLGMSVLAVGFGFSAAVSNPFSIGTAQRLAGIPMFSGAPFRILVLAACYGLYVL
                     FLTRHARRVERRAAAASPEDAGSSISAGAVPGAAADPDLSPESYRGARAFGYSMATLL
                     AVVLAAALVPGLSDYSLPIMALAFLAAGFVSSRAAGLPLARWFRAFGGGALGVLPAGV
                     LILMALSVKRIVMAGGVLDTILFGAAEAVSGASGYGAAAAMYLLTLGLNFFIGSASAK
                     AFLLMPLLAPLADLTDISRQIAVQAFAFGDGFSNMLYPTNAVLLIALSIAGVSWTKYA
                     KWVLPLQAAAFVLTMGLLMLSVAFGYG"
     gene            5711..>6386
                     /locus_tag="P5313_14625"
     CDS             5711..>6386
                     /locus_tag="P5313_14625"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY81649.1"
                     /translation="MHPALALIFLPITLLAVLAVRALGTPRAPAPGRLDPAALEPFAG
                     IENKLAALVRLPTVSRFEAAQEDPAPFRDFPRELERLFPTVHARLRRDAVGDRALLYE
                     WPGSDPALAPAILAAHYDVVPPGEIPWTRPPFSGDAADGFVWGRGSQDTKVTLTGILA
                     AAERLLGEGYRPARTLFFAFGGDEEVGGARGAAALARTLRERGVKASFLSAEGGIVAD
                     GMLAFAD"
BASE COUNT         1249 a         2134 c         2037 g          966 t
ORIGIN      
        1 cgacttcgag gccggcaaga cctattcctt cgagcctttc ctggggctca acatacggtg
       61 atcggcggcg gattggatta aaaattggcg ccaaaaggcg tttcccggcc cgggaaacgc
      121 ctttttcgtt tgacagcggg gcttgcggcc gtggtatggt ataccccagc cgcgagcttc
      181 gcggaccttg aggaaccccg cggtagatca gaacagagga tcccgattca tgatggaaac
      241 ccgcgattac gaacagcttg ccctggaatc cggcgtatat aaggacatcg agctggacat
      301 cctcatcgac accctggact cctggaaggc ccggccggga agcccctatt ccctggtgga
      361 ggtccgggac agccgggagc ccgcgggatt ctgcctcttc cagcgcgccg ccaacacgga
      421 atccacattt gatattcaca ccttcctggt gggacgggac taccggggca agggcgccgc
      481 ggaaaagctg ctggacctgc tggccgagga gatcctggcc tccgtgcggt ccgccatgat
      541 ccggatcgag acctcttcca cgaaggaaaa cgccgtggaa ccccggttct tcaccgcccg
      601 cggcttcgag accatcggcc atattcccga tttctacggt cccggaaacg attactgtat
      661 ctacgcgaag caagtggccc ggacctgaac cgatcccggg ccccgggagt ccgagaagga
      721 accgacgtat cggaccggcc gtattccctg attgaccctg tacttttcgc ccgtatcggc
      781 cgagaatgaa gggaagattc gaacgacacg cgataggatc gtaatcatga ggacgaccgt
      841 ggaagcctat tcccttcgca atgccgccgc cctagtgcag gaatcgagca ccaaacgcgg
      901 gaacctgagc gtccagtccg ggaagatatc cgcgggcaag gccgccggac ccgagatcga
      961 cctgggcggt tcgtccttcc tgtatcccgc cctgatcaac atccacgacc atttccgggg
     1021 taactacgtg ccccaggtcg gccccaacgg gaacgccttc tacatcaact ggagcccctg
     1081 ggacaacgac ctcaaggcct ccccggtgta caaggagagg gccaacatca ccgtggagca
     1141 gatgtacttc ctctcctcct acaagaacct cttcagcggg gtggccacgg ccaacgacca
     1201 cttcccccac gagatcaacg agcccttcct gccccgcctg cccatccggg tcatcgggga
     1261 gtacaccctg gcccacgaat gctcctcctt cgacctcaag tggggggacg gcattgagat
     1321 cgagcacaag cgggcggtgg agcggaactg gcccttcatc acccacctgg aggaaggatt
     1381 cgacccggaa tcccaggacg ggatcggcat tctggagcgc ctgggctgcc tggacggcca
     1441 cgacgtcttc atccactgca tcggcttctc cgacgaggac atagctaaga cggccaaggc
     1501 cggggcctcg atctcctggt gtcccgcctc gaacatgctc atgttcaacg tcacctgcaa
     1561 gatccgtaag ttcctggagg ccggggtcaa cgtctgcctg ggcacggact ccacacacac
     1621 gggatccgcc aacctccttt cggagatgaa gttcgcccgc aaggtgttcc gggacatgta
     1681 cgggaccgaa ctgccggcca agaccctcta ccgcatggtg accgcgaacc ccgccaaggc
     1741 cttccgcatg caggaccgga cgggatccct ggacaccggc aagtccgcgg acctcctggt
     1801 cctcaaggct atccatgacg acccctacga agccttggcc aaggcggagc cttcggacat
     1861 ccgactcctg accctggaag gcgtccccct cctggtggag gaagccctgg ccgagccctt
     1921 ccgggagctc ctggccaagg gctacgagac cgtcaaggtg gggggccgga aggtccatgt
     1981 ccgcggcacc cccgcgtccc tgtaccggga cgtccggaag gcgatcggct tcgacaagaa
     2041 gctggattac ctgcccttcg agccgtgatg cccgaaaact ccggggtttg tgtggaaatc
     2101 gccgcggccg tccactatac tgctatcgca cgccgcgtta cggcgagagt ggagtgagga
     2161 gcaatgccga aggcagccaa ctaccgcgca aactcggtca tctacttcca gggggacgtg
     2221 atcagcgaca agatctacat cctccaatcc ggccaggtag tggtgaacta cacggacatc
     2281 gagaccggca aggacctcag ggaggtcatc cagaccggcg agttcttcgg ggtcaagagc
     2341 gccctgggca agtatccccg ggaggagaac gccgtggtgg tctcggacgc ccaggtcctg
     2401 atcttcacgg tcccggaatt cgagcagttc gtctccgcca acacccgcat catcctcaag
     2461 atgctccggg tcttctccaa ccagctccgg cggatccaca agcaggtcga gaacctgatg
     2521 gaaaagtcgg aggccctgag cccggagatc ggcctgttcc gcaccgggga gtattacctg
     2581 aagaatcgca tgtactccca ggccaagtac gtattcagcc ggtacctcac ctattatccc
     2641 gccggcaagc tggcggacgc ggccagcaag aacctggaga cttcggaaag ctgcatggta
     2701 aagttcgggg acggcaaggg gccctccccg tcggtggcag gaccgcccgc gggcaccctg
     2761 agcgcgcatc ccggcgccgg cgccgccccc gccaagcccg ccgacaccct ccgcccccag
     2821 gcgggacagg ccctgtcgga cacggccaag gagtactaca acgccgtgag ccttttctcc
     2881 caggagaaat acaaggaagc cctgggggag ttcaagcgca tcgtggaagc caaccaggat
     2941 cccgaatacg tggtcaaggc cgccttcgac atggggcgca gcgtgttcat gctgggccag
     3001 ttcgacgcgg ccatcgccca cttcacccgc atggtccaga cctatcccaa gcatccggac
     3061 ctggcggaca tcctctacta cctgggacag tcctacgcca agaaggggga cgtggaacgg
     3121 gccaaggggt tcttccggaa gatcctgacc atggaaaagg acgaggacaa ctcgaccttc
     3181 ctccgggcca agaaggccct gcgcggcctg gaggaggccc agaatggctg acctctccgc
     3241 cttcacccgt ttcgcccgga cctaccaggc cggggagatc atcttctccg agtacgagcc
     3301 cggcgactcc ttctacctga tccagtcggg caaggttcgc atagccaaga tcgtcgggga
     3361 catcgagaag accatcgacg tcctccagcc cccggagatc ttcggggaga tggccatcct
     3421 ggaggggacc cctcgctccg ccacggcggt ggcggtggac acggtccggg tcctggagtt
     3481 caaccgggcg aatttcgagg tcctcatgat gggcaatccg cagatcgccc tgcggctcct
     3541 caagctcttc accaagcgga tctacgacca gaagcgccgg ttcatgatcc tgaccctgga
     3601 ggatccccag gcccgggtgg cggacgtctt cctgatgctg gacgagaccc aggccgccgc
     3661 cacggcgggg gccgagcagg acggggagaa gcgggagttc aaggtgacca tcgaggacgt
     3721 ggcccactgg gggggcatgg gggcttcgga ggtccagacc atcctgggcc acttcgccaa
     3781 ccagcgccgg gtggagctct accccgaccg ggtggtggtc cggaacatca gcgacttttc
     3841 ccgcttcgtg aactcccgaa gaaagaaata gaaggttccg ggactcacca cggaggacac
     3901 ggagaacacg gagaagaaaa gagaataaat attttgcgaa tattcggaca ggatggacag
     3961 gatcatgcaa ggatggacag gatatatttt tatttaagat aataaactat taatcctgtt
     4021 catctgttct aaatcctggc atcctgactc tcctcgaact ccgaaaaaac ctagaaaccc
     4081 atcgacgaag acgcgtagcg gcttcgttgc cgttcggcta cggatgaggc cccgcctcat
     4141 ccgtagccga acgcgacgga aagcatcagc aatcccatgg tcaatacgaa ggccgcggcc
     4201 tggaggggca ggacccactt ggcgtacttc gtccaggaca cccccgcgat ggagagggcg
     4261 atcaggagca ccgcgttcgt gggatatagc atgttggaga agccgtcccc gaaggcgaag
     4321 gcctggaccg cgatctgccg ggagatgtcc gtgaggtccg ccaggggagc caggaggggc
     4381 atcaggagga aggccttggc gctggcgctg ccgatgaaga agttcaggcc cagggtcagg
     4441 aggtacatcg cggccgccgc tccgtagccg gaagccccgg acacggcctc cgcagccccg
     4501 aagaggatgg tatccaggac acccccggcc atcacgatac gcttcacgct cagggccatc
     4561 aggatgagga cccccgcggg aaggaccccc agggctcccc ctccgaaggc cctgaaccag
     4621 cgggcgagcg gcaggcccgc ggcccgggag gagacgaaac ccgcggccag gaaggccagg
     4681 gccatgatcg gcagggaata gtccgacagg ccggggacca gtgcggccgc caggaccaca
     4741 gccaggaggg tggccatcga atagccgaaa gcccgggccc cccggtagct ttcgggggaa
     4801 aggtccggat cggcggccgc tccggggacg gcgccggcgg aaatcgagga acccgcgtcc
     4861 tccggagacg ccgcggccgc gcggcgctcc acgcgccggg cgtgccgggt caggaagagg
     4921 acatagaggc cgtagcacgc ggccaggacc aggatacgga acggcgcccc ggagaacatg
     4981 ggaatccccg ccaggcgttg ggcggtcccg atggagaagg gattggagac cgcggcggag
     5041 aagccgaacc ccaccgccag gacgctcatc cccagacccg tgaggacgtc ccacccgaag
     5101 gaggccgcca gggccgcggc cacgggcacc aggaggacgg actcctcgaa gacccccatg
     5161 agggatccca ggagcatccc gaagagggac accgccgcca ggagccggaa ccttcgggtc
     5221 cggtaccgcc gggccaggac ggccaccgcc gcctccagga cccgggcctc gttcaggccc
     5281 gcgaaggatc cccccgccac ggcgatgaag gcgatgatca ccagggccgt cagggcgtcc
     5341 ggggagccca ggacctccgc cggggcggtc agccagcgcc aggccgggta gtccggcctc
     5401 tccgtgaccc ggtaggatcc cgggaccagg gtcggcccgg agggaccctc gatcctctca
     5461 taggtccccg cgggcacgac ccgggtcagg atcccggcgg cgaccatgag gcccgccagg
     5521 atgagggccg agaccacgaa ggccttcgcg ccgatggaaa cgttcttcat ggagccctcc
     5581 gcggccgcgg gcctccgggg ccggccagcc tgccggggga gtataccacg gcacgcagga
     5641 aggggcgagg ttgaccggga gggtcccccg ggttagtgtt acgaaccttg tgatgccccg
     5701 gaggtacccc gtgcatcccg ccctcgcgct catcttcctt cctataaccc tcctggccgt
     5761 cctcgcagtc cgcgccctgg gcaccccccg ggcccctgcg cccggcaggt tggacccggc
     5821 cgccctggaa cccttcgcgg gcatcgagaa caagctcgcc gccctggtcc gtctgcccac
     5881 ggtctcccgc ttcgaggccg cccaggagga tcctgccccc ttccgggact tcccccggga
     5941 actggagcgc ctcttcccga cggtgcacgc ccggctgcgc cgggacgccg taggggaccg
     6001 ggccctgctc tacgaatggc cgggctccga ccccgccctg gccccggcga tcctggccgc
     6061 ccactacgac gtggtccctc ccggggagat cccctggacc cggcccccct tttccgggga
     6121 cgccgcggac ggtttcgtct ggggccgggg ttcccaggac acgaaggtga ccctgacggg
     6181 gatcctggct gccgcggagc ggctcctggg ggagggatac cgccccgcac ggaccctctt
     6241 tttcgccttc gggggggacg aggaggtcgg gggcgcccgg ggggccgcgg ccctggcccg
     6301 gaccctccgg gagcgggggg tgaaagcctc cttcctctcc gccgaggggg ggatcgtagc
     6361 ggacgggatg ctggccttcg cggatc
//
LOCUS       DAOTEI010000194         6356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_8753_length_6356_cov_4.297889, whole genome shotgun sequence.
ACCESSION   DAOTEI010000194 DAOTEI010000000
VERSION     DAOTEI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8753_length_6356_cov_4.297889"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="P5313_14630"
     CDS             <1..516
                     /locus_tag="P5313_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81650.1"
                     /translation="DFGYDVLTARTDGTGDMIPVTRIYCFEGSVVVTVLPAPDVPPEE
                     LKVKAGSMVTVERSEGKWVPSLVPVSSDIRAFWTQNDFAQAAKPSGVIDLPTYARVQA
                     NLKVKNAAIFGGVVLVGLGSVLQGVGAYAYSSGDPVLGLNAVAGGAFLSILSLPVLVF
                     GLSTNPVLAGD"
     gene            complement(530..1453)
                     /locus_tag="P5313_14635"
     CDS             complement(530..1453)
                     /locus_tag="P5313_14635"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRY81651.1"
                     /translation="MPQSVFQSIVLFILLASGFGAGKLKVLGPETVRGMSRFIVDFTL
                     PALVLVSMQKPFSPELRDEAFRMLGISFAIYLSALPLAVLWARAIRARGLERGVHEFG
                     GTFANVAFMGFPIMGAYFGKDILFDVSIYNMPFQLLAFSVGVIMLTRGTSGAPRLTLR
                     QWINPAILSTTLGFVFFLGSVKISGPLLSGLTILGDMTTPLSMAVIGAILSRMDVRGV
                     LGNLRVYLTSVYRLVLHPLAVWLVLSALGMRGQSLAVPVVLATMPVAANASILSDAYG
                     GDSETASALVFVSTAVSLVTIPLLAWTLFGL"
     gene            complement(1519..2985)
                     /locus_tag="P5313_14640"
     CDS             complement(1519..2985)
                     /locus_tag="P5313_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRY81652.1"
                     /translation="MYLTFDIGTSSVKGALVGEDGRASRVERAPVGLYRGEGESAWEA
                     EPSEWWKALAECSLRLVRRNEGPIRAVAVSGNGPTLVPADSSGNPVGRAISWMDRRAR
                     DQADRISAVLGFPLDPSFYLPKALWFLEHREAEFRRTARFFSCPEWAAFRLCGEACTY
                     LADPYYERYTWGGPAASELGLDASRFPPYAAPGRVLGKVLPEAARDTGIPEGTPVVTA
                     FPDFLASLLGSGAVEPGIACDRAGTSEAFNLCSDAPCADWSFLSLPHAVPGLWNISGG
                     LSTSGKALEWYARAAGFPADEEPPARKVFRAAETSVPGARNLLFLPFLAGERAPLWRR
                     DLRGAFLGLSVDHGPGDLARAVAESIGFGLRLTSQGLAERGHVARLVRVSGNPARNAF
                     LTRLKADILGLPVEVPELPDCELSGDAAACAAALGDSADLKEAAARLVRVESRFEPDP
                     RLRGEYDRLYYVFADAVAALIPLSERLSPESGSPGSGG"
     gene            complement(2991..3458)
                     /locus_tag="P5313_14645"
     CDS             complement(2991..3458)
                     /locus_tag="P5313_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRY81653.1"
                     /translation="MSSDHSFRIGDLARMFGITARTVRYYEELGLIEPENRDEGEHRR
                     YSDRNVIRLKRVQQLKDYGLTLAEISELFDLARKDRSGESARRGLAGKYRERLEDALR
                     RKEALDKYIEDLSWHIEQLERTPDFFECPGASCASCRWAGRCDVRSLLTREGA"
     gene            3610..4662
                     /locus_tag="P5313_14650"
     CDS             3610..4662
                     /locus_tag="P5313_14650"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY81654.1"
                     /translation="MNPVRRSWIAAAAVLALFASCAKGPDDSSSRPAVPAAFKGVAVF
                     VPGVVSGSPIYEMLAAGARRAAESVPGTTFRVVEAGFNQAEWLDKLAALAASGEFDLI
                     VTSNPALPELCAKVAESYPAMRFFVADAYLPGNPAIHTVLYNQLEQGYIVGYLAGLIT
                     TSGLPGANRAKKAGMVIAQTYPTLDKLIRPGFEAGLKAVDPGISLEVRVVGNWYDANR
                     SAELTSSLIDGGVDVILPIAGGAGQGVLSASKERGTYAVWFEGKGYGLAPGTILGCAV
                     LDQERLVRERVGALLSGDKSLFGKADVVGASEGYIGFDPEGDAYKALPSELRSRFEKA
                     LTDLRTGSPAFRIDSL"
     gene            4666..6177
                     /locus_tag="P5313_14655"
     CDS             4666..6177
                     /locus_tag="P5313_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY81655.1"
                     /translation="MAAEPVLEVRDLSKTFPSTGTVALSGVSLSLSAGEIHAVVGENG
                     AGKSTLARILAGLDRPDSGRILVRGREVRFRRPRDAERLGIGMVPQQSLLAPGLSVAE
                     NVVLGHEPRLLGILLDRRRAYYETALASANFSFPLDPGSRISRLSPPERREAEIVRAL
                     ARGGDVLILDEPTSILTESEAETLYGLLKRLRDAGASVILISHRVREVLSAADRITIL
                     RNGSVVDTILPAETDDCDLALRMASSASCFTADGPAESGGQAVFRFLGANFRDSERVT
                     LRDLGFTVRRGEIYGIAALAGNGLGALEDLACGERICDSGAVELLGRNLVDWPRDELR
                     ASALSYLPTDRDGRGLCLGATILENLAARIGSPGDWSRRGRAARRAEAFRLLEDAGGR
                     APPDAPIDMLSGGNRQRVLCVRELGTFTPAVLAANPTQGLDPRAQEETWNRLRSRARE
                     GAGILLLTSSVDELFSVADRVAVLYRGRLTELGIRTGSVTAEAVTRLLTGAAA"
     gene            6174..>6356
                     /locus_tag="P5313_14660"
     CDS             6174..>6356
                     /locus_tag="P5313_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81656.1"
                     /translation="MKPGKEGRRPGFPGSGTALALGAALGAAALYILAAAEEPGAAFR
                     AFFLSPFARPTVLANLI"
BASE COUNT          966 a         2197 c         2253 g          940 t
ORIGIN      
        1 gatttcgggt acgacgtcct gaccgcccgg accgacggga ccggagacat gattccggtc
       61 acccggatct actgcttcga gggttccgtg gtcgtgaccg tccttcccgc ccccgacgtc
      121 cctccggagg agctcaaggt caaggccggc agcatggtga cggtggaacg gtccgagggg
      181 aagtgggtgc cgtccctggt gcccgtatcc tcggacatac gggcgttctg gacccagaac
      241 gacttcgccc aggcggccaa gccttcgggg gtgatcgacc tgcccaccta cgcccgggtc
      301 caggcgaacc tgaaggtgaa gaacgccgcg atcttcgggg gggtcgtcct ggtggggttg
      361 ggctctgtgc tccagggggt cggggcctac gcctattcct ccggggatcc cgtgctcggc
      421 cttaacgccg tggccggggg cgccttcctg agcatcctct ccctgccggt cctcgtgttc
      481 ggcctgtcca cgaaccccgt cctggcgggg gattaagcga aagccgctcc tagagcccga
      541 agagggtcca ggccaggagc ggtatcgtga ccagggagac cgcggtggac acgaacacga
      601 gggcgctggc ggtctcggaa tccccgccgt aggcgtccga gaggatggac gcgttcgccg
      661 ccacgggcat ggtcgccagc accaccggga cagccaggga ctggccgcgc atccccaggg
      721 ccgacaggac cagccagacg gccaggggat gaaggaccag ccgatagacc gatgtaaggt
      781 acacacggag gtttcccagg acgccccgga cgtccatccg cgacaggata gccccgatca
      841 cggccatgga caggggggtg gtcatgtctc ccaggatggt gagtccggac agcagggggc
      901 cggagatctt gacggacccc aggaagaaga cgaatcccag ggtggtggac aggatggcgg
      961 gattgatcca ctgccggagg gtgagccggg gggcgccgga cgttcccctg gtcagcatga
     1021 tcacccccac ggaaaacgcc agcagctgga agggcatgtt gtagatcgat acgtcgaaga
     1081 ggatgtcctt cccgaagtac gcccccatga tgggaaagcc catgaaggcc acgttggcga
     1141 aggtcccccc gaactcgtgc acccccctct ccagtccccg agcccggatc gcgcgggccc
     1201 acagcacggc cagggggagg gcggacaggt agatcgcgaa ggaaatgccc aacatccgga
     1261 aggcctcgtc ccggagctcg ggggaaaagg gtttctgcat ggagaccagt accagggcgg
     1321 gcagggtgaa gtccacgatg aaccgcgaca tcccccggac cgtctccgga cccaggacct
     1381 tgagctttcc ggcgccgaat cccgacgcga ggagtatgaa caggacgatg ctctggaaca
     1441 cggactgggg catcgggcct ccgaaggcct cggggtttcc gggccggtct ctaatctacc
     1501 gagtttcggg attcgtttct atccgccgga gcccggggat ccggactccg gggagagtcg
     1561 ttcggacagg gggatgagag ccgccacggc gtccgcgaac acgtagtaca accggtcgta
     1621 ctccccgcga agccgcgggt ccggctcgaa tcgggactcc acccggacca gccgggcggc
     1681 ggcctccttc aggtccgcgc tgtcccccag ggccgcggcg caggcggcgg cgtcgcctga
     1741 tagctcgcag tccggcagtt cgggaacctc caccggcagt cccaggatgt cggccttgag
     1801 ccgggtcaaa aaggcgttcc gggccgggtt cccgctgacc cgcaccagcc gggcgacatg
     1861 cccgcgctcc gccaggccct gggacgtaag gcgtaggccg aagccgatgg attccgccac
     1921 cgcccgggcg aggtccccgg gtccgtggtc cacggagagc cccaggaagg ctccccggag
     1981 atcccggcgc cacagggggg cgcgctcccc cgccaggaag ggcaggaaga ggaggttccg
     2041 cgcccccggc acggacgtct ccgccgcgcg gaagaccttg cgggccgggg gctcctcgtc
     2101 cgcggggaaa cccgcggcgc gggcgtacca ctccagggcc ttcccgctgg tggacagccc
     2161 tccggagatg ttccagagcc ccgggacggc gtggggcagg gagaggaagc tccagtccgc
     2221 gcagggggcg tcggagcaga ggttgaaggc ttcgctggtg cccgcccgat cgcaggcgat
     2281 cccgggttcc acggccccgg agcccaggag ggacgccagg aagtccggga aggcggtgac
     2341 gacgggggtg ccctcgggga ttcccgtgtc ccgggcggcc tccgggagca ccttccccag
     2401 cacgcggccg ggagcggcgt agggcgggaa ccgggacgcg tccaggccga gttctgaggc
     2461 ggccgggcct ccccaggtgt aacgttcgta gtagggatcc gccaggtagg tgcaggcctc
     2521 cccgcagagc cggaaggcgg cccactccgg gcaggagaag aaccgggcgg tccggcggaa
     2581 ctccgcctcg cggtgttcca ggaaccagag ggccttgggc aggtagaagg agggatccag
     2641 gggaaatccc aggacggcgg agatccggtc cgcctgatcc cgggcccgcc gatccatcca
     2701 ggagatcgcc cggcccacgg gatttccgct cgaatccgcg ggtaccaggg tgggaccgtt
     2761 cccgctgacc gcgacggccc ggatcgggcc ttcgttccgt cgtaccagcc ggagggagca
     2821 ctcggccagg gccttccacc actccgaggg ctccgcctcc caggcggact cgccctcccc
     2881 gcggtacagc cccaccggtg cccgttccac ccgggacgcc cggccgtcct ccccgaccag
     2941 ggctcccttg acggaggagg ttccgatatc gaaggtcagg tacacggggg ctaggcccct
     3001 tcccgggtca ggagggaccg gacgtcgcag cggcccgccc agcggcagga cgcgcaggac
     3061 gcgcccggac actcgaagaa gtccggggtg cgttccagct gctcgatatg ccaggaaagg
     3121 tcctcgatgt acttgtccag ggcttccttg cggcgcaggg cgtcctccag gcgttcccgg
     3181 tacttgcccg ccaatccccg gcgggcggat tccccgctcc ggtccttgcg ggccaggtcg
     3241 aagagttccg agatctccgc cagggtgagt ccgtaatcct tgagctgctg gacccgcttg
     3301 agccggatca cgttgcggtc cgagtagcgc cgatgctccc cctcgtcccg gttttccggc
     3361 tcgatgagcc cgagctcctc gtagtaccgg acggtgcggg cggtgatgcc gaacatgcgg
     3421 gcgaggtcgc cgattcgaaa cgagtggtcg gaagacatga atcgcaccct taacgtaaac
     3481 ttggcggcgc gtcaggatcg ggatcctgta cccccgccgc gggacctcag tatactcccc
     3541 ccgacagcgt gtgaaaagcc ggccggcttt ttcgttccgg tccggcagca gctgtgtcag
     3601 gagagtacga tgaacccagt ccgcagatcc tggatcgccg ccgccgcggt ccttgccctt
     3661 ttcgcgtcct gcgccaaggg gccggacgat tcctcctccc ggcccgcggt cccggcggcc
     3721 ttcaagggtg tcgccgtgtt cgtgcccggg gtggtctccg gaagccccat ctacgagatg
     3781 ctggccgcgg gggcccgccg ggccgccgag tcggtcccgg gcacgacctt ccgggtcgtg
     3841 gaggccggct tcaaccaggc cgagtggctg gacaagctgg ccgccctggc ggcctccggc
     3901 gagttcgacc tgatcgtcac ctcgaacccg gccctgccgg agctctgcgc caaggtcgcg
     3961 gaatcgtatc ccgcgatgcg gttcttcgtg gccgacgcct atcttccggg gaatccggcg
     4021 atccacacgg tgctctacaa ccagctcgag cagggataca tcgtcgggta cctcgcggga
     4081 ctgatcacca cctcgggcct ccccggtgcc aaccgggcca agaaggccgg gatggtcatc
     4141 gcccagacct atccgaccct ggacaagctg atccgccccg ggttcgaggc gggactcaag
     4201 gccgtggatc ccgggatcag cctggaggtc cgggtggtcg ggaactggta cgacgccaac
     4261 cggagcgcgg aactgacgtc ctccctgatc gacggcgggg tggacgtgat cctgcccatc
     4321 gcgggcgggg ccggacaggg ggtcctctcg gcctccaagg agaggggaac ctacgcggtc
     4381 tggttcgagg gcaaggggta cggcctggcc ccggggacga tcctgggctg cgccgtcctg
     4441 gaccaggagc gcctagtccg ggagcgggtg ggcgccctcc tttccgggga caagtccctc
     4501 ttcggcaagg ccgacgtcgt cggggcgtcg gaggggtaca tcgggttcga cccggaggga
     4561 gacgcctaca aggccctgcc gtcggagctc cggtcccggt tcgagaaggc cctgacggac
     4621 ctgcgaacgg gatcccccgc cttccggatc gacagcctgt agtccatggc ggcggaaccc
     4681 gtcctcgaag tccgggacct ttccaagacc ttcccctcga cgggcacggt ggccctgtcg
     4741 ggcgtatccc tgtccctgtc ggccggggag atccacgccg tggtggggga gaacggggcg
     4801 ggaaagagca ccctggcccg gatcctcgcg gggctggacc ggcccgactc aggccgcatc
     4861 ctggtccggg gtcgggaggt gcggttccgc cgtccccggg acgccgagcg gctcggcatc
     4921 ggcatggtcc cccagcagtc cctcctggcc cccggtcttt cggtggcgga gaacgtggtc
     4981 ctggggcacg agccccggct cctggggatc ctcctggatc gtcgcagggc ctactacgag
     5041 accgccctgg cctcggcgaa tttctccttt cccctcgatc cgggctcccg gatctcccgc
     5101 ctctcccccc cggagcggag ggaggcggag atcgtgcggg ccctggctcg gggcggggac
     5161 gtcctcatcc tggacgagcc caccagcata ctcacggagt ccgaggcgga gaccctctac
     5221 ggcctcctca agcgcctgag ggacgcggga gcctcggtga tcctgatcag ccaccgggtc
     5281 cgggaggtcc tgtccgcggc ggaccgcatc accatcctgc ggaacggttc cgtcgtggat
     5341 acgatcctcc cggcggaaac cgacgactgc gacctcgccc tccgcatggc ctcctccgcc
     5401 tcttgcttca ccgcggacgg tcccgcggaa tccggcgggc aggcggtctt ccggttcctc
     5461 ggggcgaact tccgggattc cgagcgggtg accctccggg acctgggctt cacggtccgc
     5521 cggggggaga tctacgggat cgcggccctg gccggcaacg gcttaggcgc cctggaggac
     5581 cttgcctgcg gggaacggat ctgcgattcc ggagcggtgg agctcctggg acggaacctg
     5641 gtggactggc cccgggacga gctccgggct tcggccctgt cctaccttcc cacggatcgg
     5701 gacggcaggg gtctctgcct gggagccacg atcctggaaa acctggcggc ccggatcggg
     5761 tccccggggg attggagccg ccggggccgg gccgcccggc gagccgaggc gttccggctg
     5821 ctcgaggacg cgggtggccg ggcgccgccg gacgcgccca tcgacatgct ctccgggggc
     5881 aaccgccagc gggtcctctg cgtccgcgag ctcgggacct tcacccccgc ggtgcttgcg
     5941 gccaacccca cccagggcct ggatccccgg gcccaggagg agacctggaa ccgcctgcgg
     6001 tcccgggccc gggaaggcgc ggggatcctc ctgctcacgt ccagcgtgga cgagctcttt
     6061 tccgtggccg accgggtggc ggtcctctac cggggccgcc tgaccgaact ggggatccgg
     6121 accggctccg tcaccgcgga ggccgtcacg aggctcctca cgggggccgc cgcgtgaaac
     6181 ccgggaagga aggccggagg ccgggcttcc ccggatccgg gacggccctg gccctggggg
     6241 cggccctggg cgccgccgcc ctctacatcc tcgccgccgc ggaggaaccc ggggccgcct
     6301 tccgggcctt tttcctctcg cccttcgccc gtccgacggt cctggccaac ctgatc
//
LOCUS       DAOTEI010000195         6227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_9002_length_6227_cov_4.099482, whole genome shotgun sequence.
ACCESSION   DAOTEI010000195 DAOTEI010000000
VERSION     DAOTEI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9002_length_6227_cov_4.099482"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="P5313_14665"
     CDS             complement(<1..1821)
                     /locus_tag="P5313_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81657.1"
                     /translation="MTASERLAASAAEALREAIADAGGNEVFAAGRLDEEGLVTEITV
                     AARGRKDEVLALASYLDRGDVILHNHPSGILEPSQADLSVASSVGERGIGSYIVDNAV
                     ERIYVIAEPARRRALVALDEDEIAGVLEPGGKLSQRIPSYEARESQVGLARHVTRALN
                     EGFVLAAEAGTGVGKSYAYLLPAFAWAARNGDRVVVSTATLNLQRQLMDKDIPVVREL
                     FRKKLKAVLAKGRGNYLCRNRLAEALDEEGLLAGDEHPLRRIAAWADQTPTGDRADLS
                     FWPDEETWSRVRSESDACLGLRCSRRGDCFVLRAKREAADANLIVANHHLLFADLAAR
                     MGGAGYEAAAVLPPFQALVFDEAHAMESSAVSYFSEELTRFAVLRALGRLFRTKRERR
                     FGVLVRVQNLKGLPADLFAKVPGQIAAVRAAADDLDARTVSFLAAEPSLRVRRREPAW
                     EEPLFAPLSALEKALVALAQLLKDLFDPLPEEAGSDPSVYEAKLILRRLSDLASLCAQ
                     FREPQEYPESVLWVSREKTSKGEPFAKYTATPLDLSGMMNEAVFHPYRSVVCTSATLA
                     VGGSFDYWKRRVGLARTDEEVETAVYPSPFPYEENALVALP"
     gene            complement(1818..2630)
                     /locus_tag="P5313_14670"
     CDS             complement(1818..2630)
                     /locus_tag="P5313_14670"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRY81658.1"
                     /translation="MKHGYGERVARAAAAIPPGFRPRSAVILGSGLSGIVEGLGFKEI
                     PYSEIPEFPRPTVQGHKGLLCLSKDAAILAGRFHYYEGHPMDDVVLPVFFLKALGVEN
                     LILTNAAGGINTKYVPGDLVLIQDHINLMGTNAFIGPNPVDPSGKPLGERFFDMSEVY
                     SKDLRALAWSCAEKPLKEGVYAGLTGPSYETPSEIRMLKTLGADLVGMSTVPEATAAR
                     YLGMRVLGISCVTNMAAGISKTPLAHAEVVEVGKRAEAELKALLLKVLAKLA"
     gene            complement(2627..4549)
                     /locus_tag="P5313_14675"
     CDS             complement(2627..4549)
                     /locus_tag="P5313_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY81659.1"
                     /translation="MKIRDLSLKAKVLAITLAGIAVIAVLIALVYIGDISKMAYSSIL
                     EKSRIIVMNAEAARDVMAEKLELGVIRDLEELAKEGDREKIIAAVPILSAIEVASRNA
                     ESNRYLLRVPKFSPRNPANIPGGPEIEALKALESGNLEEYVVREAFQVRYFRPIRLTR
                     ECMICHGDPAGSLDPVGGVREGWKVGEVRGAFEIISSLGAASEARSQAIRNIAAFTLT
                     VMALLGASLFLVVRLVLRPIGGYVRAFEQSAGGDLTVRAAVSARDEIGKVADYFNDFI
                     GTLESMVQRVKSESDGVGAISQDLAASSEETAASLHEIRTNTEHMKDKIVRLDEEISD
                     SASSARDLRDYIGRLAELIQNQAAAITQSSASIEQMGASINNIAKTAEEKLRISTELE
                     STAQGGQSEMEETEQVIKKVAQSAGVILEMIEIIQGIASKTNLLAMNAAIEAAHAGEY
                     GKGFAVVADEIRNLAESSAESAAQITHSLGEVMGYIKVSEASTEKTGTAFSQIVDKVK
                     DVADSMSEMKNTTHELSLGAREVLEALGTLVSTTEEVKGSSREMNDRVAAISDAMGRM
                     SDYSSETKNGMEEITIGINEIYKAAEAISRAGTENSESVRRLRDLVARFKVSEGTGPE
                     ADGTAAGTSSKGRESA"
     gene            4830..>6227
                     /locus_tag="P5313_14680"
     CDS             4830..>6227
                     /locus_tag="P5313_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81660.1"
                     /translation="MTIGQKASISTLISVILFAGFAVLAYSGLFETVQTRFYNPRVAS
                     GVNKTLENLARESETYHSVLSGRFKSILALDFVRRSVLPNQSSEDIFNRSSAFGRLAQ
                     ETPGLLGIRLVDVDGRRIHFSTYEGDVLRREEFRQVYRNYGDGDVSLEVLGVPDGDPG
                     AVRILESANSFVYVFPFRDSFDAYRGTALFQVARSGLQEYLVKVGALPVGEDFLLLDG
                     RGLLFGAPRQGRPELVGRVVELWSGRLDSEPIPVGSMESSEELVLFSRRGETGLYGRL
                     APASWFILPNSMRIVFLAAFFVTAYLLSFLLLNLRQEDLTVISHRVKSLQIGLLEEYL
                     DRKGDVDFARWKSELESRKGEVRTEIRAGIGRVRKSRRKQVDDLIDRGWDEILQILGA
                     RAPASESQIDIARIEGLLKQALSNGSLVVNAAPAPVSGKKPKPVQAKAEAVEEVEAVE
                     EAEAVEEAEAVEEVEA"
BASE COUNT          954 a         2065 c         2108 g         1100 t
ORIGIN      
        1 cggcagggcc accagggcgt tctcctcgta ggggaagggc gaggggtaga ccgcggtctc
       61 gacctcctcg tccgtgcggg ccaggcccac ccggcgcttc cagtagtcga aggacccccc
      121 gacggccagg gtcgcggagg tgcagaccac ggagcggtag ggatggaaga ccgcctcgtt
      181 catcatcccc gagaggtcca ggggggtggc ggtgtacttg gcgaagggct cgcccttgct
      241 ggtcttctcc cgggagaccc agagcacgga ctcagggtat tcctggggct cccggaactg
      301 ggcgcacagg gaggccaggt ccgagagcct gcgcaggatc agcttggctt cgtagaccga
      361 gggatccgag ccggcctcct cgggcagggg gtcgaaaagg tccttcaaga gctgggccag
      421 ggccaccagg gccttttcca gggcggacag gggcgcgaac aggggttcct cccaggccgg
      481 ctcccggcgt cggacccgca gggagggctc ggcggccagg aaggagacgg tccgggcgtc
      541 caggtcgtcc gcggccgccc gcaccgcggc gatctgtccg ggcaccttgg cgaagaggtc
      601 cgcgggcagg cccttcaggt tctggacccg taccaggacc ccgaagcggc gctcccgctt
      661 ggtgcggaag aggcgtccca gggcccggag caccgcgaac cgggtgagtt cctcggaaaa
      721 gtagctgacc gcgctggact ccatggcgtg ggcctcgtcg aagaccaggg cctggaaggg
      781 gggcaggacc gcggcggcct cgtagcccgc cccgcccatc cgggcggcca ggtccgcgaa
      841 caggaggtgg tggttggcca cgatgaggtt ggcgtccgcg gcttcccgct tggcccgcag
      901 gacgaagcag tcccccctgc gggagcagcg gaggcccagg catgcgtcgg actcggaccg
      961 gacccggctc caggtctcct cgtcgggcca gaaggagagg tccgcccggt cccccgtcgg
     1021 ggtctggtcc gcccaggccg cgatgcgccg gaggggatgc tcgtccccgg ccaggagccc
     1081 ttcctcgtcc aaggcttcgg ccagacggtt ccggcagagg tagttccccc ggcccttggc
     1141 cagcacggcc ttgagcttct tgcggaagag ctcccggacc acggggatgt ccttgtccat
     1201 gagctggcgc tggaggttga gggtggccgt ggagaccacg acccggtccc cgttgcgggc
     1261 cgcccaggcg aaggccggaa ggaggtaggc gtaggacttg ccgaccccgg tccccgcctc
     1321 ggcggccagg acgaagcctt cgttcagggc ccgggtcacg tggcgggcca gcccgacctg
     1381 gctctcccgg gcctcgtagg acggtatgcg ctgggagagc ttgcccccgg gctccaggac
     1441 cccggcgatc tcgtcctcgt ccagggccac cagggcgcgt cggcgcgcgg gctcggcgat
     1501 gacgtagatc cgctccaccg cgttgtccac gatgtaggac ccgatcccgc gctcccccac
     1561 ggaggaggcc acggagaggt ccgcctggct gggttccagg atcccggaag ggtggttgtg
     1621 gaggatgacg tccccccggt ccaggtagga ggccagggcc aggacctcgt ccttgcgacc
     1681 ccgggcggcc acggtgatct ccgtcaccag gccctcctcg tccaggcgcc ccgccgcgaa
     1741 gacctcgttc ccgccggcgt cggctatggc ctcccggagc gcctcggcgg cggaggcggc
     1801 caggcgctcc gaggcggtca tgccagcttc gcgagcacct tcaggagcag ggccttgagc
     1861 tcggcctccg cgcgcttgcc cacctcgacc acctcggcat gggccagggg ggtcttgctg
     1921 atccccgcgg ccatgttggt cacgcaggag atccccagga cccgcatgcc caggtagcgc
     1981 gcggcggtgg cctcgggaac cgtggacatg cccaccaggt ccgcgcccag ggttttcagc
     2041 atgcggatct ccgagggggt ctcgtaggag ggcccggtca ggcccgcgta cacgccctcc
     2101 ttgaggggtt tttccgcgca gctccaggcc agggcgcgga ggtccttgga atagacctcg
     2161 ctcatgtcga agaaccgctc ccccaggggt ttgccggagg ggtccacggg attcggtccg
     2221 atgaaggcgt tcgtgcccat gaggttgatg tggtcctgga tgaggaccag gtccccgggc
     2281 acgtacttgg tgttgatccc ccccgccgcg ttcgtgagga taaggttctc cacccccagg
     2341 gccttgagga agaagacggg gaggaccacg tcgtccatgg gatggccctc gtagtaatgg
     2401 aagcgtcccg ccaggatcgc ggcgtccttg gaaaggcaca ggagcccctt gtgtccctgc
     2461 accgtgggcc gcgggaactc cgggatctcg gaatagggta tctccttgaa tcccagaccc
     2521 tccacgatgc ccgaaagccc cgagcccagg atgacggcgc tgcggggccg gaatcccggg
     2581 ggaatggcgg cggccgcccg tgcgacgcgt tctccgtaac cgtgtttcat gcggattccc
     2641 tgcctttcga cgatgttccg gcggccgtcc cgtccgcctc cggcccggtt ccctcggaaa
     2701 ccttgaaccg ggccaccagg tcccgcagcc gccggacgga ttcggagttc tccgtccccg
     2761 cccgggagat ggcctcggcg gccttgtaga tctcgttgat cccgatggtg atctcctcca
     2821 tcccgttctt ggtctccgag gagtagtcgg acatccggcc catggcgtcc gagatggccg
     2881 ccacccggtc gttcatctcc cggctggagc ccttcacctc ctcggtggtg ctcaccaggg
     2941 tgcccagggc ctccaggacc tcccgggccc ccagggacag ctcgtgggtc gtgttcttca
     3001 tctccgacat ggaatccgcc acgtccttga ccttgtccac gatctggctg aaggcggtgc
     3061 cggtcttctc cgtgctggcc tcggagacct tgatgtaccc catgacctcg cccagggagt
     3121 gggtgatctg ggccgcgctt tcggcgctgg actccgccag gttgcggatc tcgtcggcca
     3181 ccaccgcgaa gcccttgccg tactcgccgg cgtgggcggc ctcgatggcg gcgttcatgg
     3241 ccaggaggtt ggtcttggag gcgatcccct ggatgatctc gatcatctcc aggatgaccc
     3301 ccgcggactg ggcgaccttc ttgatgacct gctcggtctc ctccatctcc gactgcccgc
     3361 cctgggccgt ggactccagt tccgtggaga tccggagctt ctcctccgcg gtcttggcta
     3421 tgttgttgat ggaggcgccc atctgctcga tgctggcgct ggattgggtg atggccgcgg
     3481 cctggttctg gatgagctcc gccaggcgtc cgatatagtc ccggaggtcc cgggcggagg
     3541 aggcgctgtc ggagatctcc tcgtccaggc gcacgatctt gtccttcatg tgctcggtgt
     3601 tcgtccggat ctcgtggagg ctggccgcgg tctcctcgga actggcggcc aggtcctggg
     3661 aaatggcgcc cacgccgtcg gactcggact tgacccgctg gaccatggac tccagggtcc
     3721 cgatgaagtc gttgaagtag tccgccacct ttccgatctc gtcccgggcg ctcacggcgg
     3781 cgcggaccgt caggtcgcct cccgcggact gttcgaaggc acggacatat cccccgatgg
     3841 gccgtaacac cagccggacg accaggaaga ggctggctcc gagcagggcc atgaccgtca
     3901 gggtgaaggc ggcgatgttg cggatggcct gggaccgggc ctcggaggcg gctcccaggg
     3961 aactgatgat ctcgaaggct ccgcggacct ccccgacctt ccagccttcc cggaccccgc
     4021 ccaccggatc caggctgccc gcgggatccc cgtggcagat catgcattcc cgggtcaggc
     4081 ggatcgggcg gaaataccgg acctggaagg cctcccggac gacgtattcc tccagattgc
     4141 ccgattccag ggctttcagg gcctcgatct cgggacctcc ggggatgttg gcggggttcc
     4201 ggggggagaa cttcggaacc cgcagcaggt aacgattgga ttccgcgttc cggctggcca
     4261 cttcgatggc ggagaggatg ggcaccgccg cgatgatctt ctcccggtcc ccctccttgg
     4321 ccaactcctc cagatcccgg atgaccccca gttcgagctt ttcggccatg acgtcccggg
     4381 cggcctcggc gttcatgacg atgatccggc tcttctccag gatggacgag tatgccatct
     4441 tgctgatgtc tccgatgtat accagggcga tcagcaccgc gatcacggcg atgcccgcca
     4501 gggttatcgc cagaaccttg gctttcaggc tcaggtcgcg tatcttcatc cggactcctt
     4561 gaccgtttta cgagggtttg atcccgcgga tccagtatag tcgggacggt ccggtgtcgc
     4621 aagttcgggt tttccccggg aaagcgcccg tatgcccccg gccctttgtt caaacggggc
     4681 ggccccgata taatgtggga aggttttccg gtgtggatac gggtgctcgc ggccgatatt
     4741 gaaacgaagg ggtcgattcc tctttgcggg caacggggcc ccgggccccg gacggtccag
     4801 gtagaaggtc ctccaggaat aggaaccaga tgacgattgg ccagaaggct tcgataagca
     4861 ccctcatcag cgtgatcctc ttcgcgggat tcgctgtcct ggcctattcg gggcttttcg
     4921 aaactgtcca gacccggttc tacaatcccc gcgtggcgtc gggtgtgaac aagaccctgg
     4981 aaaacctggc ccgggaatcg gaaacctacc attccgtcct gtccggccga ttcaaatcca
     5041 tcctggccct ggatttcgta cgaaggagcg tacttccgaa ccagagctcc gaggatatct
     5101 tcaatagatc ctccgccttc ggccgattgg cccaggagac ccccggtctc ctaggtatcc
     5161 gcctcgtgga tgtcgacggc cgacgtatcc attttagcac ctacgaaggg gacgtcctgc
     5221 gccgggagga gttccggcag gtataccgga actacgggga cggcgacgtt tccctggagg
     5281 tcctgggcgt gccggacggc gatcccgggg cggtccggat cctggaatcc gccaattcct
     5341 tcgtgtacgt cttccccttc cgggattcct tcgacgcgta ccgcggaacc gccctgttcc
     5401 aggtggcccg gtccgggctc caggaatacc tggtcaaggt gggcgccctg ccggtcgggg
     5461 aggatttcct cctcctggac ggccggggcc tcctcttcgg agccccgcgt caaggccgtc
     5521 cggagctggt cggccgggtg gtcgaactct ggtcgggacg cctggactcg gagcccatcc
     5581 ccgtcggctc catggaaagt tccgaggaat tggtactctt ctcccgccgc ggggaaaccg
     5641 ggctgtacgg ccggctggcc ccggcgtcct ggttcatcct gcccaattcc atgcggatcg
     5701 tgttcctggc cgcctttttc gtgaccgcat atctcttgtc cttcctcctg ctgaacctac
     5761 gccaggaaga cctgacggtc atttcccatc gggtgaagag cctgcagatc ggcctgctcg
     5821 aggaatacct ggaccgcaag ggagacgtgg acttcgcccg ctggaagagc gagttggaat
     5881 cccgcaaggg cgaggtccgg accgagatcc gggccgggat cggccgggtc cggaagtccc
     5941 gccggaagca ggtggacgac ctgatcgacc ggggctggga cgagatcctg cagatactcg
     6001 gagcccgggc ccccgcctcg gaatcccaga tcgacatcgc ccggatcgaa ggactcctca
     6061 agcaggccct gtcgaacggc tccctggtcg tgaacgccgc gccggcaccc gtttccggga
     6121 agaaaccgaa gcccgtgcag gcaaaggccg aagccgtcga agaagtcgag gcggtcgaag
     6181 aggccgaagc cgtcgaggaa gccgaagcgg tcgaagaagt cgaggcg
//
LOCUS       DAOTEI010000196         6198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_9055_length_6198_cov_4.353410, whole genome shotgun sequence.
ACCESSION   DAOTEI010000196 DAOTEI010000000
VERSION     DAOTEI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9055_length_6198_cov_4.353410"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /locus_tag="P5313_14685"
     CDS             complement(<1..2139)
                     /locus_tag="P5313_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRY81661.1"
                     /translation="MKANIHYFSQKDTIKKTVDPEKLGQRGRQANEFAELGLPILPGF
                     IVDAEVAGHLAKEDVAAAVRPVIKKIEDVVGKKYADPKNPLLFKMVISPNLAVSDYPT
                     LHNFGLVRGNVAGFNTWVGARFGAHEVLFLIRGMLKVEERVADMEGRAKDLDKIRAAL
                     AKAEKALDVEKFSDSSEAVIQAYEALLPKGFFDDPWVQLDLAMKRVSRLLELDEQDAD
                     DTAILVQPMVYGNYGKDSASGAYFTRNVVTGEKKLQGDFYQERFNEIGAQGQDVNKIA
                     PAYLKDLEAVARKLEDHLREIRQIRFTIENKKLWLIEQRQVVAKSTQADIQLLLDLNK
                     RRIVDDAFVVNSIKPGRLNEILHPIVDLASVKKLKGVTGGIAGAPGAAIGRVYFTTEA
                     LLDAHRVAQQKGQDKRMILCMPATFADDVKAIEVATGVLSTEGGYSAHASVVARQYGK
                     VSLVKPDLRIRGKKATLGDMTISEGDYLTINIPFYGEPMVWFGKADLIEPKPDESGLL
                     EFIALAKKSMKDFHVRANSDSPRDAALALSFGAEGIGLCRTEHMFFNEKRINTFREMI
                     LADSKKDRLKALAKLQGMQREDFYGLFKTMAGKEVTIRLLDAPLHEFLPHNENEMEIF
                     LKYMGAGKGGTSLSKSEIRARFDALAEFNPMLGHRGCRIAVSYPEIYEMQVRAIFEAA
                     YKLKSEKVDVRPEIMIPIIMNSSELKLIVYG"
     gene            complement(2162..3214)
                     /locus_tag="P5313_14690"
     CDS             complement(2162..3214)
                     /locus_tag="P5313_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81662.1"
                     /translation="MGCQFENLANRDGFNASVRQIKSTLGVVERVGAKENLADLIAEL
                     LEDKSITQESVPPLVSMVLVDKFGYVSLSENLPSEGDIGSVASAVAGWNAVDLVAVYY
                     HPDLGPIAVNPKNPAHLARLEHLNKYELLVVYAGAFGRKADPMLFGTAARFLIDLFAG
                     KAVKPPAALLKGECSYKARPSAATKAAPAAKPVAKLAAAKKGAGAGKKTSAKAVPVPP
                     PVPKKAAAVAEESAAAAPALRPGVRMTPMYSVLVTNELFHNGNVEAWKRIIESYRTKH
                     PDLDVIVYYDRERITNLNALFKWGKVKRGTTIQFAVVGEQIGDVAKLLRYLQQGASHL
                     FEAFLKGPVNSVLPLF"
     gene            complement(3369..5435)
                     /gene="cfpA"
                     /locus_tag="P5313_14695"
     CDS             complement(3369..5435)
                     /gene="cfpA"
                     /locus_tag="P5313_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HRY81663.1"
                     /translation="MGAIDLPKSPNVFHPEKPSAVGSRNSLAQEGRDQQKEVNQLLEE
                     ETNKVMHHLRARLPKEVLERLDVMGGVKEKLYNYFNQNYQNMFNRYIVTTEDEMVKKV
                     RNFIDKEEMKSLARYTPKEIAELLDQVGGADKFNTGEIEKSVVNMYGHLQGHIQRGVN
                     ELENLTNSLLRQKTDVGAFIRGENAYSVVKCSFKDNIYKPKTVADVKLSVNILDSELI
                     SPIFHYQVTVEYLIKDLIAKHILDQIDKEIDKLKDQLVEEGKEEITDTEIIFEKMKKV
                     KEYTDDDVDNPKSKRYTIIAKDLMDRINNLRAEIDPTTFDQLNIRENLKKIVDIENIR
                     NRGFNTAVNSLTSILDTSKMGYQYIENLKNARELIIREYEDTDYAHLPDERYKIRLQY
                     YDNAQLLEERKAYDVQVKSFETEIEHIWDVLEMRYVDNKFLKRITDFDDLAKIYRRKI
                     KRKIKDQKGEPSYEDIAKVWDEIAFVKPAETDVEKMNRTYLFEKDKLRSKLIMMKDKL
                     KTMYSYQYPIERRVMEERLAFLEKEFFRFDYMINPYHIQPGILLDVDICSIKRKKATL
                     DGMANVLNEFLHGVSKGFQDAAFAAFSRRRSTVREDIAQSFGPVEDPHAHPANDYLSM
                     LNADSAPALEAPAPAPVTVKAAPARTRGRPSKGVVDTGRKKAAPKAKASGRGRPKGRE
                     GGIREL"
     gene            5671..>6198
                     /locus_tag="P5313_14700"
     CDS             5671..>6198
                     /locus_tag="P5313_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY81664.1"
                     /translation="MKRCRLALVLALFAAAAAFPGDERTLPVDFIFLVDRSLSMKPVL
                     EEVKRYLAGDVIGPLVVPGDSVTLLAFYGKVERIWQGRIGVEADKADLIRSLHRLAPD
                     GRYTDIGRALDFLDPVLTELDAADRLKYILLLTDERQEAPPDSRYQSGDYTIAHRYLT
                     YVNRRDFGPFRAITIG"
BASE COUNT          996 a         1870 c         1959 g         1373 t
ORIGIN      
        1 gccgtacacg atcagcttga gctccgagga attcatgatg atgggaatca tgatctccgg
       61 ccggacatcg accttctcgg acttgagctt gtaggcggcc tcgaagatgg cccggacctg
      121 catctcgtag atctcgggat agctcaccgc gatgcggcag ccccggtggc ccagcatggg
      181 gttgaactcc gccagggcgt cgaagcgcgc ccggatctcg gacttggaga ggctcgtgcc
      241 ccccttgccc gcccccatgt acttgaggaa gatctccatc tcgttctcgt tgtggggcag
      301 gaactcgtgc aagggggcgt ccaggagacg gatggtgact tccttgcccg ccatggtctt
      361 gaacaggccg tagaagtcct ccctctgcat gccctggagc ttggccaagg ccttgaggcg
      421 gtccttcttg ctgtccgcca ggatcatctc ccggaaggtg ttgatccgct tctcgttgaa
      481 gaacatgtgt tccgtgcggc aaagtccgat gccctccgcc ccgaacgaga gggccagggc
      541 cgcgtcccgg gggctgtccg agttggcccg gacgtggaag tccttcatgg acttcttggc
      601 caaggcgatg aactcgagga ggccggactc gtcgggtttg ggctcgatca ggtccgcctt
      661 gccgaaccat accatgggtt ccccgtagaa ggggatgttg atggtcaggt agtcgccctc
      721 ggagatggtc atatccccca gggtggcctt cttgccccgt atacggagat ccggcttgac
      781 cagggagacc tttccatact gccgggccac gacggaggcg tgggccgagt acccgccctc
      841 ggtggacagg actcccgtgg ccacctcgat ggccttgacg tcgtccgcga aagtggcggg
      901 catgcagagg atcatccgct tgtcctgacc cttctgctgg gccacccggt gggcgtccag
      961 caaggcctcg gtggtgaaat agacccgccc gatcgccgct cccggagctc ccgcgatgcc
     1021 cccggtaacg cccttcagct tcttcacgga agcgaggtcc acgatgggat ggaggatctc
     1081 gttcaggcgg cccggcttga tggagttgac cacgaaggca tcgtccacga tcctgcgctt
     1141 gttgaggtcc agaaggagct ggatgtccgc ctgggtgctc ttggccacca cctggcgctg
     1201 ctcgatcagc cacagcttct tgttctcgat ggtgaaccgg atctggcgga tctcccgcag
     1261 gtggtcctcc agcttccggg ccaccgcctc caggtccttg aggtaggccg gggcgatctt
     1321 gttgacgtcc tggccctggg ctccgatctc gttgaaccgc tcctggtaga agtcgccctg
     1381 gagcttcttc tccccggtga cgacgttccg ggtgaaatat gcccccgagg cggagtcctt
     1441 gccgtagttt ccgtagacca tgggctggac taggatggcc gtgtcatccg cgtcctgctc
     1501 gtccagttcc agcaggcggg agacccgctt catggccagg tcgagctgga cccagggatc
     1561 gtcgaagaag cccttgggca ggagggcctc gtaggcctgg atcaccgcct ccgaggaatc
     1621 ggagaacttc tccacgtcca gggccttttc cgccttcgcc agggccgccc ggatcttgtc
     1681 caggtccttg gcccggccct ccatgtccgc gacccgctcc tcaaccttga gcatgccccg
     1741 gatcaggaag aggacctcgt gggccccgaa ccgcgccccg acccaggtgt tgaagcccgc
     1801 cacgttgccc cggaccaggc cgaagttgtg gagggtcggg taatccgaga cggccaggtt
     1861 gggggaaatc accatcttga agaggagggg gttcttggga tccgcgtact tcttcccgac
     1921 cacgtcctcg atcttcttga tcaccggccg taccgcggcg gccacgtcct ccttggccag
     1981 gtgtccggcc acctccgcgt ccacgatgaa gcccggcagg atgggcagcc cgagctcggc
     2041 gaactcgttg gcctgccggc cccgctggcc gagcttctcg ggatccaccg ttttcttgat
     2101 cgtatccttc tggctgaaat aatgtatgtt cgctttcatg acggctcctg gttatcgtcg
     2161 attagaacag cggcaatacg ctgttcacgg gccccttcag gaaggcctcg aacaagtggc
     2221 tggctccctg ctggaggtat cgcaggagct tggccacgtc cccgatctgt tcgccgacga
     2281 cggcgaattg gatcgtagtg ccccgcttga ccttgcccca cttgaacagg gcattcaggt
     2341 tggtgatccg ttcccgatcg tagtagacga tgacgtccag gtccgggtgc ttggtccggt
     2401 agctctcgat gatccgtttc caggcttcca cgtttccgtt gtggaacagc tcgttcgtca
     2461 ccagcacgga atacatgggg gtcatgcgga ccccgggccg gagggccggg gccgccgcgg
     2521 cggattcctc ggcgaccgcg gccgccttct tgggcaccgg aggcggaacg ggcacggcct
     2581 tggccgacgt tttcttaccc gccccggcgc ccttcttggc ggcggcgagc ttggcgacgg
     2641 gcttggccgc gggtgcggcc ttggtagcgg ccgagggccg ggctttgtag gaacactctc
     2701 ccttgagcag ggcggcgggg ggcttcacgg cctttcccgc gaacaggtcg atcaggaacc
     2761 gtgccgcggt cccgaagagc atgggatccg ccttccggcc gaaggctccc gcgtacacca
     2821 ccaaaagctc gtacttgttg aggtgctcca gacgtgccag gtgggccgga ttcttgggat
     2881 tgaccgcgat cggacccagg tccgggtggt aatacacggc cacgaggtcc acggcgttcc
     2941 agccggcgac cgccgaggcc acgctcccta tgtccccctc ggagggaagg ttctcggaca
     3001 ggctcacgta accgaacttg tccacgagga ccatggaaac caggggaggc acggattcct
     3061 gggtgatgga tttgtcctcc aggagctccg cgatcaggtc cgcgaggttt tccttggccc
     3121 ccacccgttc cacgacgccc agggtggatt ttatttgccg taccgaggcg ttgaagccat
     3181 cccggttcgc caggttctcg aattgacagc ccatctatgc gctcctttca tccttataac
     3241 tttcgagttt cgcgcctgcc gcccgatttt tcaagtccgc ggcgggtcgt tcccccgttc
     3301 cctgccgcga tccgggaaag aaaaaggccc cccgaggaaa tcggggggcc tgtcgcggcg
     3361 aatcgtgctt aaagctccct gatcccgcct tcacgaccct tcggccggcc gcggccggaa
     3421 gccttggcct tcggagcggc cttcttcctg ccggtatcca cgacgccctt ggaagggcgg
     3481 ccgcgggtcc gggccggagc ggccttgacc gtgacgggcg cgggcgcggg ggcctcgagg
     3541 gccggggcgg aatccgcgtt cagcatggaa aggtaatcgt tggccggatg ggcatgggga
     3601 tcctccaccg ggccgaagct ctgggcgatg tcctcccgga ccgtggaccg gcggcggctg
     3661 aaggccgcga aggccgcgtc ctggaagccc ttggaaacgc cgtggaggaa ctcgttgagc
     3721 acgttggcca tgccgtccag ggtggccttc ttgcgcttga tggagcagat gtcgacgtcc
     3781 agcaggatgc ccggctggat gtggtaggga ttgatcatgt agtcgaagcg gaagaactcc
     3841 ttctccagga aggccagacg ctcctccatg acccggcgct cgatcgggta ctggtaggag
     3901 tacatggtct tgagcttgtc cttcatcatg atcagcttgg accggagctt gtccttctcg
     3961 aagaggtagg tccggttcat cttctcgacg tcggtctccg cgggcttcac gaaggcgatc
     4021 tcgtcccaga ccttcgcgat gtcctcgtac gagggctcgc ccttctggtc cttgatcttc
     4081 ctcttgatct tgcggcggta gatcttggcc aggtcgtcga aatccgtgat gcgcttgagg
     4141 aacttgttgt ccacgtaccg catctccagg acatcccaga tgtgctcgat ctcggtctcg
     4201 aagctcttca cctggacgtc gtaggccttg cgctcctcca ggagctgggc gttgtcgtag
     4261 tactgcaggc ggatcttgta gcgctcgtcg ggcaggtggg cgtagtcggt atcctcgtac
     4321 tcccggatga tgagctcccg ggcgttcttg aggttctcga tgtactgata gcccatcttg
     4381 gaggtatcca ggatggaggt cagggagttg acggcggtgt tgaagccgcg gttccggatg
     4441 ttctcgatat ccacgatctt tttgaggttt tcgcggatat tgagctggtc gaaggtggtg
     4501 ggatcgattt cggcccggag gttgttgatc cggtccatca ggtccttggc tatgatggtg
     4561 tagcgcttgg acttgggatt gtcgacgtcg tcgtccgtgt attccttcac cttcttcatc
     4621 ttctcgaaga tgatctcggt gtcggtgatc tcttccttcc cttcctcgac gagctggtcc
     4681 ttgagcttgt cgatttcctt gtcgatctgg tccaggatgt gcttggcgat caggtccttg
     4741 atgaggtact cgaccgtcac ctggtagtgg aagatcgggg agatgagctc ggagtccagg
     4801 atgttgacgg aaagcttgac gtccgccacg gtcttgggct tgtagatgtt gtccttgaag
     4861 gagcacttca ccacggaata ggcgttctcg ccccggatga aggcgcccac gtcggtcttc
     4921 tggcgcagga gggagttggt caggttctcc agctcgttca cgccgcgctg gatgtggccc
     4981 tgcaggtggc cgtacatgtt gacgacggac ttctcgatct cgccggtatt gaacttgtcc
     5041 gctccgccca cctggtccag gagttccgcg atctccttgg gggtgtaccg ggccagggac
     5101 ttcatctctt ccttgtcgat gaagttccgg accttcttca ccatctcgtc ttccgtggtg
     5161 acgatatagc ggttgaacat gttctggtag ttctggttga agtagttgta cagcttctcc
     5221 ttgaccccgc ccatcacgtc caggcgctcc aggacctcct tgggaagccg ggcgcggagg
     5281 tggtgcatga ccttgttggt ctcttcctcg aggagctggt ttacttcctt ctgctgatcc
     5341 cggccttcct gggccaggga gttcctggat ccgacggcgc tgggcttctc gggatggaat
     5401 acgttcgggc ttttcggcag atctatggca cccatgtcta actcctttat gcggtgaatt
     5461 ccgttcttac aacatagtag ccgatgatgg aaaaatgtaa agcgggatgg ggaaaaaagt
     5521 gtccatcccg atcgaattta tatggtagaa tcgcactggg tcgccggagg ggtggaaacg
     5581 tgccattttt tccgtcggac cttgcatttt caccgggtat ctcggatgct gataggagca
     5641 tttatacaag ccgtacgatt ggacaatccg atgaaacgat gtcgtctggc cctggtttta
     5701 gccttgttcg ccgcagcggc cgcgttcccg ggggatgagc ggacgcttcc ggtggatttc
     5761 atcttcctcg tggaccgttc cctgtccatg aaaccggtcc tggaggaggt caagcggtac
     5821 ctggcggggg atgtcatagg ccccctggtg gtgcccgggg attccgtgac cctccttgcc
     5881 ttttacggca aggtggagcg gatttggcag ggacggatcg gggtcgaggc ggacaaggca
     5941 gacctgatcc gctcgcttca ccggcttgcc ccggacggcc gctacacgga catcggccga
     6001 gcgctggact tcctggaccc cgtattgacg gaactggacg ccgcggatcg cctgaagtac
     6061 atcctgctcc tcacggacga gcggcaggaa gccccgccgg attcccgata ccaatcaggg
     6121 gactacacga tcgcccaccg ctatctgacc tacgtcaacc gccgggattt cgggcccttc
     6181 cgggccatta ccatcgga
//
LOCUS       DAOTEI010000197         6149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_9148_length_6149_cov_4.218247, whole genome shotgun sequence.
ACCESSION   DAOTEI010000197 DAOTEI010000000
VERSION     DAOTEI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9148_length_6149_cov_4.218247"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="gcvPA"
                     /locus_tag="P5313_14705"
     CDS             complement(<1..1121)
                     /gene="gcvPA"
                     /locus_tag="P5313_14705"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRY81665.1"
                     /translation="MPFIPNTDSDRSRMLAVIGKPSVESLFGDIPEKLRYPELDLPPA
                     LSEMEILREIEALAAKNLSSSTCAWFLGAGAYNHFIPSLVPAIASRGEFLSAYTPYQP
                     EVSQGTLQAIFEYQSMAGSLFGVEAMNASHYDGATALAEAVLMAARNAPGRETVVLPA
                     ALHPEYKEVIRTYLEPLDVKIREYAGTPAAAAASLDPSAACLVAAYPDFEGRVHDLSG
                     VADAVHAAGALLVVHADPILLGLFKSPGELGADIVTAEGQALGNAMNYGGPFLGMMGA
                     ASKYVRKMPGRIAGQASDAEGRRGFVLTLSAREQHIRREKALSNICSNQGLVMLQACI
                     YLATLGKNGLRKVAELCWHKAHYAAAEIAKLPGFSVTKD"
     gene            complement(1221..1604)
                     /gene="gcvH"
                     /locus_tag="P5313_14710"
     CDS             complement(1221..1604)
                     /gene="gcvH"
                     /locus_tag="P5313_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRY81666.1"
                     /translation="MKIDAAARYRSSHEWARKEGSLIVTGISDHAQESLGDIVFVEFP
                     EVGKTLKQGETFGVIESVKAASDLYMPMGGKIAAVNEALGGDPSAVNKDCYGAGWLIK
                     FEPTNPSEWDGLLSAGDYEKEAAAH"
     gene            complement(1638..2819)
                     /gene="gcvT"
                     /locus_tag="P5313_14715"
     CDS             complement(1638..2819)
                     /gene="gcvT"
                     /locus_tag="P5313_14715"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRY81667.1"
                     /translation="MSLRLPGFDARIALSAASPRRPSRSKEDTLKSTALHDWHAARGA
                     KMAPFAGYEMPITYPTGAVEEHLITRRSVGLFDIDHMGQIEVSGPGADDFVSRMVSAR
                     VLDMKDGDARYSLLLAEDGGVTDDLFIYRLPGRWWIVVNASNRETDYAWFRKNAPAGV
                     KVEDLSDDTYMIAVQGPRAIELLGAAADFDVPGMTRFTSAKGSLLGVPCLVGRTGYTG
                     EDGAELFFPARDAVKLWDGLLALAAKKGIEAAPIGLAARDSLRFEAGMPLHGHEISPS
                     INPVEAGFKWACDLEKDFVGKKAVTDLAEKGPARKLVGLDVTGGVPREGYEVQATDGR
                     TVGLCAAGMFCPTVKKYAANAFVPPELSKVGTPLKVVIHGKPKDAVVVKRPLYVPAYR
                     R"
     gene            complement(2901..3725)
                     /locus_tag="P5313_14720"
     CDS             complement(2901..3725)
                     /locus_tag="P5313_14720"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRY81668.1"
                     /translation="MAGSRRGSRSSSSSSSSKNSRVGAGCLIWLVALCVVFVLVFANL
                     GRIRQTLESTKFLDIVSTQGGREPSAPAPAPVAPAPQVQEEPSGKPPKKPAGGDAVTV
                     GPGTKPAPVETPPPESPAAKPPEAQPEKPGPVKTRSAALYFVKIDGDGVIARHEVKRT
                     IPATDSPLTDAIGSLLRGPGEEELRKELITLIPQGTRLLSAQVRGTTAYLNFSEAFMY
                     NSYGIEGYAGQLKQVVWTATAFPTVQDVQILVEGQRRDFLGGEGVYIGQPLSRNSF"
     gene            complement(3779..4762)
                     /locus_tag="P5313_14725"
     CDS             complement(3779..4762)
                     /locus_tag="P5313_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY81669.1"
                     /translation="MRRARRAETRPALPDLRETAGLLRRAAGIQTDFDPALRPRKPSG
                     APGGLLLLPEELPVLVLPDLHTRAGLLRRALEWIPPEGSRSVLELLDEGAAFLLLLGD
                     LFHSEGPDAPLRWERAYREALTGFALREAMDEEMARALSALDVLARTLIRAPGRAACL
                     KGNHDNSRNREGSGDHGFYKFADEGAMCAAWLSAFTPPEFREALASFEESLPLAARGA
                     RFAASHAEPAVPLSEAELIEAAPDTVEALTWTANGQALPGAAAETLEGLLGRGAAEEG
                     ARWISGHRPVEGRYSLRADGLLVQIHAPFSFRGAWVRPDGPFDPETDIFDL"
     gene            complement(4809..5939)
                     /locus_tag="P5313_14730"
     CDS             complement(4809..5939)
                     /locus_tag="P5313_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81670.1"
                     /translation="MDDEDEARLRNIVYVRLPEDFARSIGGFLLDPSIALPVETGGDP
                     ERFRPGDLVLEALLAGMLRVLAYRPDHPDADYFRRFVTTVKPSILGELSEAGILKSRN
                     GDLDVAEEIFRALRGLYPDRPESYLNLASVYEKRAQEYGRLDNPGLEEEFSQRAFDLY
                     RRLLAFEPPVPDAYYEAAFFHLKNRGYDRARELFETYATLGEDAERVARAREIVRKLR
                     TQGSLDTLFKEAYDFIRLGREEEGIGKAREFLSSYPKVWNGWFLLGWGCRRLKRWAEG
                     REAFEKAIELGAEDADTYNELAICLLELGEYRECRKRLEAALRLEPDNVKIVSNLGTL
                     ALKQGRPDEAERFFRTALELDPRDPLARSLLESLASGDGGPG"
     gene            complement(5929..>6149)
                     /locus_tag="P5313_14735"
     CDS             complement(5929..>6149)
                     /locus_tag="P5313_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81671.1"
                     /translation="DIKGKVVRYEDVEGRKDLAAVAIQFDEQQVPMNYKIHVNDYVVQ
                     IRGPRTEEGPQAPGGTPGSKGGGETDGR"
BASE COUNT          930 a         2049 c         2191 g          979 t
ORIGIN      
        1 atgtccttcg tcaccgagaa gcccggaagc ttggcgatct ccgcggcggc gtagtgggcc
       61 ttgtgccagc agagctcggc caccttccgc aggccgtttt tgccgagggt ggccaggtag
      121 atgcaggcct ggagcatgac cagtccctgg ttggagcaga tgttggacag ggccttctcc
      181 cgccggatgt gctgttcccg ggcggagagg gtcaggacga agccccggcg cccctcggcg
      241 tccgaggcct gaccggcgat gcgtccgggc atcttgcgca cgtacttgga cgccgccccc
      301 atcatgccca ggaaggggcc gccgtagttc atcgcgttgc ccagggcctg gccttccgcg
      361 gtgacgatgt ccgctccgag ttccccgggg ctcttgaaca ggcccaggag gatggggtcc
      421 gcgtggacca ccagcagggc gcccgcggca tggaccgcgt ccgcgacgcc ggacaggtcg
      481 tggacccgtc cctcgaagtc gggataggcg gcgaccaggc aggcggccga aggatccagg
      541 gaggccgccg cggcggcggg agtccccgca tactcccgga tcttcacatc caggggctcc
      601 agataggtcc ggatgacttc cttgtattcc gggtgcaggg ccgcgggcag gaccacggtc
      661 tcccggcccg gggcgttccg ggcggccatg agaacggcct ccgccagggc ggtggccccg
      721 tcgtagtggg aggcgttcat ggcctccact ccgaagagcg agccggccat gctctggtac
      781 tcgaagatgg cctgcagggt gccctgggag acctcgggct ggtagggggt gtaggcggag
      841 aggaactccc cgcggctcgc gatcgcggga accagggaag ggatgaagtg gttgtaggct
      901 cccgctccca ggaaccaggc gcaggtcgag gacgagaggt tcttggccgc cagggcctcg
      961 atctcccgga ggatctccat ttccgacagg gccgggggaa ggtcgagctc gggatagcgc
     1021 agcttctccg ggatatcccc gaagagggac tccaccgacg gcttcccgat gaccgccagc
     1081 atccggctgc ggtcggaatc cgtgttgggt atgaagggca cgattcgctc cttacggccc
     1141 cgtagggccc tgggtacgga gagccgtccg ggtaccgggc ggttcgccct cccggtagct
     1201 cggcggccgc cgggccggcc tcagtgggcg gccgcttcct tctcgtaatc cccggcggaa
     1261 agaaggccgt cccactccga ggggttggtc ggctcgaact tgatcagcca gcccgccccg
     1321 tagcagtcct tgttgaccgc cgaggggtcc ccgcccagag cctcgttcac ggcggcgatc
     1381 ttgccgccca tgggcatgta caggtccgag gcggccttga ccgactcgat caccccgaag
     1441 gtctcgccct gcttgagggt cttgcccacc tcggggaatt ccacgaacac gatgtccccc
     1501 agggattcct gggcgtggtc ggagatgccg gtcacgatca gggagccttc cttgcgggcc
     1561 cactcatggg aactgcggta tcgggctgcg gcgtcgatct tcatatcgtc tccttcagaa
     1621 tatagtgaga atatcgttca ccgacgatac gcgggaacgt acagcgggcg cttcacgacg
     1681 acggcatcct tgggcttgcc gtggatcacg accttcaggg gcgtcccgac cttggacaat
     1741 tccggaggga cgaaggcgtt ggcggcgtac ttcttgactg tggggcagaa catgcccgcc
     1801 gcgcagaggc cgacggtccg gccgtcggtc gcctgcactt cgtagccttc ccggggaacc
     1861 cccccggtca cgtccaggcc cacgagcttc cgcgccgggc ctttctccgc caggtccgtc
     1921 acggccttct tgcccacgaa gtccttttcc aggtcgcagg cccacttgaa gcccgcctcc
     1981 acggggttga tggacgggga gatctcgtgg ccgtgcaggg gcatccccgc ctcgaagcgc
     2041 agggaatccc gggcggccag gccgatcggc gccgcctcga tgcccttctt cgcggccagg
     2101 gccagcagtc cgtcccagag cttcacggcg tcccgggcgg ggaagaagag ctcggccccg
     2161 tcctcgccgg tgtagccggt gcggccgacc aggcagggga ctcccagcag ggagcctttg
     2221 gcgctggtga agcgggtcat gccgggcacg tcgaagtccg cggccgcgcc gaggagctcg
     2281 atcgcccggg ggccctggac ggcgatcatg taggtgtcgt cggagaggtc ctcgaccttg
     2341 acccccgcgg gggcgttctt ccggaaccag gcgtagtccg tctcgcggtt ggaggcgttc
     2401 accacgatcc accagcgacc cggcagccgg tagatgaata ggtcgtccgt cacgcctccg
     2461 tcctccgcca ggaggaggga gtaccgggcg tccccgtcct tcatgtccag gacccgggcg
     2521 ctcaccatgc gggagacgaa gtcgtccgcc ccggggccgg agacctcgat ctggcccatg
     2581 tggtcgatgt cgaacagtcc cacggaacgt cgggtgatga ggtgttcctc gaccgccccg
     2641 gtgggatagg ttatgggcat ctcgtacccc gcgaagggcg ccatcttggc gccgcgggcc
     2701 gcgtgccagt cgtggagagc tgtggatttc agggtgtcct ccttggaacg ggacggccgg
     2761 cggggggatg ccgccgacag ggcaattcta gcatcgaaac cggggagccg caaggacacg
     2821 aaccgccccg aatgtcacga cgatcgaggc accgggaagg atcgagacgc cgaaccttgt
     2881 acggcaataa aaacgacccc ctagaaggaa ttccgggaaa ggggctggcc gatatagacc
     2941 ccctcgccgc ccaggaaatc ccggcgctga ccctccacca ggatctgcac gtcctggacc
     3001 gtcgggaagg ccgtggccgt ccagaccacc tgcttgagct ggccggcgta tccctcgatg
     3061 ccgtaggagt tgtacatgaa ggcctccgag aagttcaggt aggcggtggt gccgcgcacc
     3121 tgggcggaca ggaggcgcgt cccctgggga atgagggtga tcagctcctt ccggagctcc
     3181 tcctccccgg ggcccctgag cagggagccg atcgcgtccg tcaggggaga atccgtggcg
     3241 gggatcgtgc gcttcacctc gtgccgcgcg atgaccccat ccccgtcgat cttcacgaaa
     3301 tagagggcgg cgctccgagt cttcaccggt ccgggttttt ccggctgtgc ctccgggggc
     3361 ttggccgcgg ggctctcggg cgggggagtc tccacggggg cgggcttcgt cccgggaccc
     3421 acggtcacgg cgtccccgcc cgcgggcttc ttcgggggtt ttccggaggg ctcctcctgc
     3481 acctgggggg cgggagccac gggcgcggga gccggcgcgg agggttcccg gcctccttgg
     3541 gtggagacga tgtccaggaa cttcgtggat tccagggtct gccgaatcct gcccaggttg
     3601 gcgaagacca ggacgaagac cacgcagagg gcgacgagcc agatcaggca tccggccccg
     3661 acccgggaat tcttcgagga tgaggacgac gaggaggaac gggagccgcg tcgggaacca
     3721 gccatgggac catgatacga catgggaggg ggatcggtaa acagtggacc ggggccggtc
     3781 acaggtcgaa aatatcggtc tccgggtcga agggaccgtc ggggcggacc caggcgcccc
     3841 ggaaggagaa gggcgcgtgg atctggacca ggagtccgtc cgcacggagg gagtaccggc
     3901 cttccacggg ccggtgcccg gagatccagc gggccccctc ctccgcggcg ccgcgaccca
     3961 ggagcccctc cagggtctcc gccgcggctc ccggaagggc ctggccgttc gcggtccagg
     4021 tcagggcttc cacggtgtcc ggcgccgcct cgatcagctc cgcctcggag agggggacgg
     4081 cgggctccgc gtggctggcc gcgaagcggg cgccccgggc cgccaggggc agggactcct
     4141 cgaaggaggc cagggcctcc cggaattccg ggggcgtgaa ggccgagagc caggcggcgc
     4201 acatggcccc ctcgtcggcg aacttgtaaa acccgtggtc cccggagccc tcccggttgc
     4261 gggagttgtc gtggttgccc ttgaggcagg cggcccgtcc gggagcccgg atcagggtcc
     4321 gggccaggac atccagggcc gagagggccc gggccatctc ctcgtccatg gcctcccgga
     4381 gggcgaaccc ggtcagggct tcccggtagg cccgttccca tcggagcggg gcgtcggggc
     4441 cctcggaatg gaagagatcg cccagcagca ggaggaacgc ggctccctcg tccaagagct
     4501 ccaggacgga tcgggatcct tccgggggaa tccattccag ggcccgccta aggagtcccg
     4561 cccgggtgtg caggtccggg aggaccagga cggggagttc ctcgggaagg aggaggaggc
     4621 ctcccggcgc cccggagggt ttcctggggc gaagggcggg atcgaagtcc gtttggattc
     4681 cggcggccct gcggagaagg ccggccgtct cccggaggtc cgggagggcg ggccgggttt
     4741 ccgcccgtcg cgcccgccgc acgagctcgt cggcccggcg tcgccgcgcg gcgcccggga
     4801 atccggtcct agcccgggcc gccatcgccc gacgccaggg actccaggag gctccgggcc
     4861 agggggtccc gggggtccag ctccagggcc gtgcggaaga acctctcggc ctcgtccggc
     4921 cgtccctgct tcaaggccag ggtgcccagg ttggagacga tcttgacgtt gtccggctcc
     4981 aggcggaggg cggcttccag gcgcttgcgg cattcccggt attccccgag ttccaggagg
     5041 cagatggcca gctcgttgta ggtgtccgcg tcctcggctc ccagctcgat cgccttctcg
     5101 aaggcttccc ggccctccgc ccagcgtttg agccgccggc agccccagcc caggaggaac
     5161 catccgttcc agaccttggg ataggacgat aggaattccc gggccttccc gatcccctcc
     5221 tcttcccttc ccaggcggat gaagtcgtag gcttccttga acagggtatc caggctgccc
     5281 tgggtccgga gcttccggac gatctcccgg gcccgggcga cccgctccgc gtcctcgccc
     5341 agggtcgcgt aggtctcgaa gagctcccgg gcccggtcgt agccccggtt cttgaggtgg
     5401 aagaaggcgg cctcgtagta ggcgtccgga accgggggct cgaaggccag gaggcgccgg
     5461 tagaggtcga aggcccgctg ggagaactcc tcctccaggc cggggttatc caggcgcccg
     5521 tactcctggg cccgtttctc gtacaccgag gcgaggttga ggtaggactc gggccgatcc
     5581 gggtagaggc ccctgagggc gcggaagatc tcctccgcca cgtccaggtc cccgttccgg
     5641 cttttcagga tccccgcctc cgacaactcc cccaggatgg agggcttcac ggtggtcacg
     5701 aagcgccgga agtagtccgc gtcgggatgg tcggggcggt acgccaggac cctcagcatc
     5761 ccggccagga gggcctccag gaccaggtcc ccgggccgga agcgctccgg gtcgcccccg
     5821 gtctccacgg gcagggcgat ggagggatcc aggaggaacc cccctatgga gcgggcgaag
     5881 tcctcgggga ggcggacgta cacgatgttc cggagccggg cctcgtcttc atcgtccatc
     5941 ggtttctccg ccgcccttgg agcccggagt ccccccggga gcctgcgggc cttcctcggt
     6001 cctgggtcct cggatctgaa cgacatagtc gttgacgtgg atcttgtagt tcatgggtac
     6061 ctgctgctcg tcgaactgga tggccaccgc cgccaggtcc ttccgcccct cgacgtcctc
     6121 gtagcggacc accttgccct tgatgtcca
//
LOCUS       DAOTEI010000198         6103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_9253_length_6103_cov_4.231316, whole genome shotgun sequence.
ACCESSION   DAOTEI010000198 DAOTEI010000000
VERSION     DAOTEI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9253_length_6103_cov_4.231316"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /gene="lon"
                     /locus_tag="P5313_14740"
     CDS             complement(<1..2399)
                     /gene="lon"
                     /locus_tag="P5313_14740"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRY81672.1"
                     /translation="MSLLDSLKPRESVELPLVYVRDMVVFPHALAPLFASTRFSVGAV
                     DAALAADKRVFVSMLKDTPDDKSPDIRVQEFGTVARVLQQVRLPDGSSRLLVEGEARA
                     RLRKTVYRKEWLGALVETLPEDDFQGPDADALVQVLRRDFSVYADLVKKIPGESVQVV
                     NKAEDPRRLCDLVSHALQVNAEKKQELLAILPMKDRMEALAVVLALENEQLTLQKRIT
                     QKVRARIDRSQKEYFLQEQIKEINKELGKEGEESEQAELEKQILSKSPPEEVVAKARK
                     ELARLQKLQPLSPEAGVLRTYCEWIADLPWSAATQDNRDLDRARAILDEDHYGMKKAK
                     ERILEFIAVRQLKAHMKGPILCFVGAPGTGKTSLGKSVARALGRNFVRVSLGGVRDEA
                     EIRGHRKTYVGALPGKIIQSLRKADSVNPVFLLDEIDKMSSDFRGDPASALLEVLDPE
                     QNSTFTDHYLEVPYDLSGVMFITTANSLHNVPYPLLDRMEVIEIPGYSEYEKLEIARH
                     FIVPKQLAENGLADSRVAIRNDAVLEIIRHYTMESGVRSLEREIAHVIRKIAQKAVDK
                     GYARDPSRLAEFSCAVTAKGIQGLLGKRIHDDDLVYRDPNPGVAYGLAWTEMGGTLLP
                     VETSSFEGEGELILTGNLGDVMKESARAALSYIEARAPAFGLSPADFRRRTLHIHVPE
                     GAIPKDGPSAGITLAASLLSALTGVPVLPGFAMTGEITLTGRVLPIGGVKEKLLAAHR
                     NKLPKVLLPEGNRKDMDDVPKEVSADTELVFVSTVEEALKALFPAQLAERSRPASRRS
                     VR"
     gene            complement(2396..>3068)
                     /locus_tag="P5313_14745"
     CDS             complement(2396..>3068)
                     /locus_tag="P5313_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY81673.1"
                     /translation="ENVSITRDVSGEGVQQALLKIIEGTVANVPPQGGRKHPQQEYLR
                     IDTTNILFVCGGAFVGLDKMIENRVSKAALGFGAEVKGRKDRDLHEIFTHLHPEDLIK
                     YGMIPEFIGRLPIQVNLEDLTLEDLTRIMTEPKNSIIKQYQESLRIDGVELVFEEDAI
                     AAVAEQAIKRRTGARGLRSIVETMMLDIMFDIPSLEGAKRVIVTRDVVEGTCKPSVEV
                     LAKSA"
     assembly_gap    3069..3078
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3079..3627)
                     /locus_tag="P5313_14750"
     CDS             complement(<3079..3627)
                     /locus_tag="P5313_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HRY81674.1"
                     /translation="MARPKSGANGSDTRECSFCGKSAEFAKRLIAGPGVYICDECVQV
                     CNQILSEDDVHLTANFSEDVPKPREIKDYIDQYVVGQEYAKKVLSVAVYNHYKRIANS
                     GKVQGGVEIEKANVLLIGPTGSGKTLLARTLAKKLKVPFAIADATTLTEAGYVGEDVE
                     NILLKLIQNAGGNISAAERGIIY"
     gene            complement(3602..4225)
                     /gene="clpP"
                     /locus_tag="P5313_14755"
     CDS             complement(3602..4225)
                     /gene="clpP"
                     /locus_tag="P5313_14755"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRY81675.1"
                     /translation="MDEKMIYVVPSVVEQTGLTERAYDIFSRLLKDRIVFIDGEINDQ
                     NADMIVAQLLFLESQDPEKDIDLYINSPGGSVTAGLAIYDTIQHIRPDVKTICLGQAS
                     SMGALLLACGSAGKRLALPSSRVLIHQPWGGVSGQASDISIQAREIIRLKRITVEYFA
                     RHTGKDVDTVARDMERDLFMSAEEALTYGLVDKILERRKHGKAEERS"
     gene            complement(4248..5606)
                     /gene="tig"
                     /locus_tag="P5313_14760"
     CDS             complement(4248..5606)
                     /gene="tig"
                     /locus_tag="P5313_14760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRY81676.1"
                     /translation="MIASKKIERLPDSAARLTVTVGAKEARAEYQALLAEYSKKARID
                     GFRTGKVPVAVLERKFGDEMRVEAMGRILEKGVEEALQGETLVPLAYSGPSLEGDPAF
                     ELDKDFTYSVTYDVFPEVKPGDWKGIEIEVPQVSIAKADEDRELQEIRERNAVVVEKD
                     SKAATAKGDVVTVDYRELAADGSTVTGSEREDFVFEIGTGYNLFKFDDDVVGMKKSDE
                     KTVEKTYPADFENKDLAGTSRRISVKVKQIKVKKLPDLDDELAQDVSEKFKTLEDLKA
                     DLRKNMERRMEARLRQLKEKAIVEALLARSEAELPKSMVDAELAMRWRNFVQQTTGGD
                     EGQMARLLQMTGRTPQSIFDEWRPSAEKAIRTRLVLEKLTEEGKYEATEEDQEREYAK
                     MAEESSLSVDEVKAEYKKHDALEYLKDRIREDKLMEAILAEAKVKKGKKTAFVDLFKD
                     SE"
     gene            complement(5772..5843)
                     /locus_tag="P5313_14765"
     tRNA            complement(5772..5843)
                     /locus_tag="P5313_14765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5809..5811),aa:Gly,seq:gcc)
     gene            complement(5884..5964)
                     /locus_tag="P5313_14770"
     tRNA            complement(5884..5964)
                     /locus_tag="P5313_14770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5929..5931),aa:Leu,seq:tag)
     gene            complement(6041..6102)
                     /locus_tag="P5313_14775"
     tRNA            complement(6041..6102)
                     /locus_tag="P5313_14775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6075..6077),aa:Lys,seq:ctt)
BASE COUNT          913 a         1985 c         1987 g         1208 t
ORIGIN      
        1 cgtacggagc ggcgggatgc gggacgggac cgctccgcca gctgggccgg gaagagggcc
       61 ttcagggcct cctccacggt ggagacgaag acgagctccg tgtccgcgct cacttccttg
      121 gggacgtcgt ccatgtcctt gcggttcccc tcgggcagga ggactttagg taacttgttc
      181 cggtgtgcgg ccaggagctt ctccttgacg ccgccgatgg gcaggacccg gccggtcagg
      241 gtgatctccc cggtcatggc gaagccgggc aggacgggca cccccgtcag ggcggacagg
      301 agggaggccg ccagggtgat ccccgcggag ggtccgtcct tggggatggc cccctcgggg
      361 acgtggatat gcagggtcct gcggcggaag tccgcggggg aaaggccgaa ggcgggagcc
      421 cgggcttcga tgtaggacag ggccgcccgg gcgctttcct tcatcacgtc ccccaggttg
      481 ccggtcagga tgagctcgcc ctccccctcg aaggaggagg tctccacggg caggagggtg
      541 cctcccatct cggtccaggc gagtccgtag gccaccccgg gattcggatc ccggtatacg
      601 aggtcgtcgt cgtggatgcg cttcccgagg agtccctgga tgcccttggc ggtgacggcg
      661 caggagaact ccgcgagccg ggagggatcc cgggcgtagc ccttgtccac cgccttctgg
      721 gcgatcttcc ggatcacgtg ggcgatttcc cgctccaagg accggacccc ggactccatg
      781 gtgtagtggc ggatgatctc caggaccgcg tcgttccgga tggccacccg ggagtccgca
      841 aggccgttct ccgcgagctg cttgggcacg atgaagtgcc gggcgatctc cagcttctcg
      901 tactcgctgt agcccgggat ctcgatcacc tccatccggt ccaggagggg gtaggggacg
      961 ttgtgcaggg agttggcggt ggtgatgaac atcaccccgg aaaggtcgta gggcacctcc
     1021 aggtagtggt ccgtgaaggt ggagttctgt tccgggtcca ggacttcgag cagggcgctg
     1081 gcggggtctc cccggaaatc gctggacatc ttgtcgatct cgtccaagag gaagacgggg
     1141 ttgaccgagt cggccttgcg cagggactgg atgatcttgc ccgggagggc tccgacatag
     1201 gtcttccggt gtccccggat ctccgcctcg tcccgcacgc cccccaggga aacccggacg
     1261 aaattccggc ccagggcccg ggccacggac ttgcccaggg aggtcttgcc cgtgccgggg
     1321 gcgcccacga agcagaggat ggggcctttc atgtgggcct tgagctggcg cacggcgatg
     1381 aactccagga tccgctcctt ggccttcttc atcccgtagt ggtcctcgtc caggatggcc
     1441 cgggcacggt ccaggtcccg gttgtcctgg gtagccgcgg accagggcag gtccgcgatc
     1501 cattcgcagt aggtcctgag gaccccggcc tcgggggaca ggggctggag cttctgcagg
     1561 cgggccagct ccttccgggc cttggcgacc acctcctcgg gaggggactt ggacaggatc
     1621 tgcttctcca gctccgcctg ctcgctctcc tctccctcct tgcccagctc cttgttgatc
     1681 tccttgatct gttcctggag gaagtactcc ttctgggagc ggtcgatccg ggcgcgcacc
     1741 ttctgggtga tgcgcttctg gagggtgagc tgctcgttct ccagggccag gacgacggcc
     1801 agggcctcca tccggtcctt catggggagg atggccagga gctcctgctt cttctccgcg
     1861 ttgacctgga gggcgtggga gacgaggtcg cagaggcggc gggggtcctc ggccttgttg
     1921 acaacctgga cggattcccc gggtatcttc ttgaccaggt ccgcgtatac ggagaagtcc
     1981 cgtcttagca cctggaccag ggcgtccgcg tcgggcccct ggaagtcatc ctccggaagg
     2041 gtctccacca gggcgcccag ccactccttg cggtacacgg tcttccggag ccgggcccgg
     2101 gcttccccct ccaccaggag ccgggagctt ccgtcgggaa gccggacctg ctgaaggacg
     2161 cgggcaacgg tcccgaactc ctggacccgg atgtccgggg acttgtcgtc gggggtgtcc
     2221 ttgagcatgg agacgaaaac ccgcttgtcc gcggccaggg ccgcgtccac ggcgcccacg
     2281 gagaagcggg tcgaggcgaa caggggggcc agggcgtggg gaaagacgac catgtcccgg
     2341 acgtagacca ggggaagctc cacggactcc ctcggcttca gggaatcgag aaggctcatg
     2401 cgctcttggc caggacttcc accgagggct tgcaggtccc ctccaccacg tcccgggtca
     2461 caatgacccg cttggcgccc tccaggctgg gtatgtcgaa catgatgtcc agcatcatgg
     2521 tctccacgat ggaccggagg ccccgggccc cggtccggcg cttgatggcc tgctcggcca
     2581 ccgcggcgat ggcgtcctcc tcgaagacca gctccacacc gtcgatgcgc agggattcct
     2641 gatactgctt gatgatggag ttcttgggct ccgtcatgat ccgcgtgaga tcctccaggg
     2701 tcaggtcctc gaggttgacc tggatgggca ggcggccgat gaactcgggg atcatgccgt
     2761 acttgatcag gtcctcggga tggaggtggg tgaagatctc gtggaggtcc cggtccttcc
     2821 ggcccttgac ctcggccccg aagccgaggg cggccttgga cacccggttc tcgatcatct
     2881 tgtccaggcc cacgaaggcc cccccgcaga cgaacaggat gttcgtggtg tcgatccgga
     2941 ggtactcctg ctggggatgc tttcgtcccc cctggggcgg gacgttggcc acggtgccct
     3001 cgatgatctt gagcagggcc tgctggacgc cctctcccga tacgtcccgg gtgatggaga
     3061 cgttctcgnn nnnnnnnngt agatgatccc gcgctccgcg gcggaaatgt tccccccggc
     3121 gttctggatc agcttcagca ggatgttctc aacgtcctcg cccacgtacc cggcctcggt
     3181 cagggtggtg gcgtccgcga tggcgaaggg gaccttgagc ttcttggcca gggtccgggc
     3241 gagcagagtc ttcccggagc ccgtgggtcc gatcaaaagg acgttggcct tctcgatctc
     3301 caccccgccc tggacctttc cggagttggc gatgcgcttg tagtggttgt agaccgccac
     3361 ggacaggacc ttcttggcgt attcctggcc caccacgtac tggtcgatgt agtcctttat
     3421 ctcccggggc ttggggacgt cctcggagaa gttggccgtc aggtggacgt cgtcctccga
     3481 gaggatctgg ttgcagacct ggacgcactc gtcgcagatg tacaccccgg gacccgcgat
     3541 gagacgcttg gcgaactcgg cgctcttccc gcagaaggag cactcccgcg tgtcgctccc
     3601 gttagctccg ctcttcggcc ttgccatgct tcctccgctc caggatcttg tccaccagcc
     3661 cgtacgtcag ggcctcctcg gcggacatga agaggtcccg ttccatgtcc cgggccacgg
     3721 tgtccacgtc cttcccggtg tggcgggcga agtattccac ggtgatgcgc ttcagccgga
     3781 tgatttcccg ggcctggatg ctgatgtccg acgcctgccc ggagactcct ccccagggct
     3841 ggtggataag gacccgggag gaggggaggg ccaggcgctt gcccgcgctc ccgcaggcca
     3901 ggagcagggc ccccatggag gaggcctggc ccaggcagat ggtcttcacg tccggccgga
     3961 tgtgctggat ggtgtcgtag atggccagtc ccgcggtcac cgaacccccg ggggagttga
     4021 tgtacaggtc gatgtccttc tccggatcct ggctctccag gaacaggagc tgggccacga
     4081 tcatgtccgc gttctggtcg ttgatctctc cgtctatgaa tacgatccgg tccttcagca
     4141 ggcgggagaa gatgtcgtag gccctctcgg tcaggcccgt ctgctccacc acgctcggga
     4201 ccacgtagat cattttttcg tccatccgta cacctcgcgg aagtaagtta ctcagaatcc
     4261 ttgaacaagt ccacgaaggc cgtcttcttg cccttcttga ccttggcttc cgccaggatc
     4321 gcctccatga gcttgtcctc ccggatgcgg tccttcaggt actccagggc gtcatgcttc
     4381 ttatattcgg cttttacctc gtccaccgat agggagctct cctccgccat cttagcgtac
     4441 tccctctcct ggtcctcctc ggtggcctcg tacttgcctt cctcggtgag tttctccagg
     4501 accaggcgcg tgcggatcgc cttttcggcc gagggcctcc actcgtcgaa gatggactgg
     4561 ggggtcctgc cggtcatctg gagcagccgg gccatctggc cctcgtcccc gccggtggtc
     4621 tgctggacga agttccgcca ccgcatggcc agttcagcgt ccaccatgga cttgggaagt
     4681 tccgcctcgg accgggccag gagggcttcc acgatggcct tctccttgag ctggcgcagc
     4741 cgggcttcca tcctgcgctc catgttcttc cggaggtccg ccttgaggtc ctccagggtc
     4801 ttgaacttct cggagacgtc ctgggccagc tcgtcgtcca ggtccgggag cttcttgacc
     4861 ttgatctgct tgaccttcac ggagatccgg cgggaggtgc cggccaggtc cttgttctcg
     4921 aagtccgcgg ggtaggtctt ctcgacggtc ttctcgtcgc tcttcttcat cccgaccacg
     4981 tcgtcgtcga acttgaagag gttgtacccc gtgccgatct cgaagacgaa gtcctcccgc
     5041 tcgctgccgg tcacggtgga gccgtccgcc gcgagctccc ggtagtccac ggtcacgacg
     5101 tcgcccttgg cggtcgcggc cttggagtcc ttctccacca cgactgcgtt ccgctcccgg
     5161 atttcctgga gttcccggtc ctcgtccgcc ttggcgatgg acacctgggg aacctcgatc
     5221 tcgataccct tccagtcccc gggcttgacc tccgggaaga cgtcgtaggt gacggaatag
     5281 gtgaagtcct tgtccagctc gaacgcggga tccccctcca gggaggggcc ggaataggcc
     5341 aggggcacca gggtctcgcc ttggagggct tcctccaccc ccttctccag gatccggccc
     5401 atggcctcca cccgcatctc gtccccgaac ttgcgctcca ggacggctac ggggaccttg
     5461 cccgtgcgga agccgtcgat ccgggccttc ttggagtact cggccaggag ggcctggtat
     5521 tcggcgcgcg cctcctttgc gcccacggtc accgtgaggc gggctgcgga atcgggaagg
     5581 cgctcgatct tcttgctggc aatcatgtgc gtatcctctt ttcctggcat agtatagcaa
     5641 ggaggcgcct aacggaagcc tccggttcga accgggacgc cgaagcccga ccgcgtacgg
     5701 acgggcgtct gcggttccgg ggaaaggcaa aaaaaaggcg atccgaacgt atccggatcg
     5761 ccttcaccgt aagcgggaaa cgggagtcgg acccgcgaca tcgaccttgg caaggtcgcg
     5821 ctctaccact gagctattcc cgcaagtgct tcccatccct gtccgcgggc aacgacccgc
     5881 gtttgcgaga gaagggactt gaaccctcac gccttccggc accagatcct aagtctggcg
     5941 tgtctgccat tccaccactc tcgcgcgctt cccgggggag cgccgtagcg gtcggtgcgg
     6001 gaccgatccg gcgatcaccg ttccgttccc gtacctttga gccgcgcagg gatcgaacct
     6061 gcgacccgca gattaagagt ctgctgctct accagctgag cta
//
LOCUS       DAOTEI010000199         6007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_9444_length_6007_cov_4.292675, whole genome shotgun sequence.
ACCESSION   DAOTEI010000199 DAOTEI010000000
VERSION     DAOTEI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9444_length_6007_cov_4.292675"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="P5313_14780"
     CDS             <1..931
                     /locus_tag="P5313_14780"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRY81677.1"
                     /translation="AQVSINLTDYHATPLHVVYETVREEAEKLGLLVTGSEIVGLLPL
                     EPLAEAGRFYLRRAGKSGGAPEAELLETAVQSLGLDSVAPFDANKKVVEYAVRSPSPL
                     VSRTVASFADEVSADSPAPGGGSVAALAGSLGAALAAMVANLTVGKKGYEAAWKDLDA
                     LALRAQDVKVRLLAAVDDDTNAFNDVLAAQKLPKGTEEEKTARAAAIQAGYRSATLVP
                     LSTAQACLEAARVSESAAVLGNRASVTDAGVGALLAAAGAEGAVLNVLINLGSIEDPE
                     WAAKVRAEAEALAAEARAVRDRTLVKVREVMGG"
     gene            complement(978..2003)
                     /gene="rlmN"
                     /locus_tag="P5313_14785"
     CDS             complement(978..2003)
                     /gene="rlmN"
                     /locus_tag="P5313_14785"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRY81678.1"
                     /translation="MPRRLAGLLPHEIAEDCGFDRPFRGIQVFKWLSGGALSFQDMTN
                     LPGPERERLTLLHPSLLGTAVDGRFRDEDGTVKLRLRLPDGAAVETVLLTDIEGRKTA
                     CISTQVGCPMACAFCKTGTLGFLRNLEAFEIVEQYLHLTRMFGRPSNLVFMGMGEPLL
                     NLPEVRRSVEILSHPEGMGLSLRKITISTCGLPEGIRDLADSGPHVRLAVSLTSAVDE
                     VRSGLMPVNRSQGLSELRDALAYYQAKTGDRITLEAVLIGGVNDRRQDMEAIVEWSRP
                     LKVQVNLIPWNPVPDLPYREPSRRAVEEAVTLLESAGIPVTRRMRRGRGVLGACGQLG
                     ETLRAPD"
     gene            complement(2006..3154)
                     /locus_tag="P5313_14790"
     CDS             complement(2006..3154)
                     /locus_tag="P5313_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81679.1"
                     /translation="MTNQSDDRIPGADPAETPSGSQPPELVEISELSKKGYQYLKENR
                     VRDAEECFRKILETEPENNYALVGIGDAARKRGIYREAVDSYKKCLVYHPGNNYALFG
                     LADCYKALNQYQMAIEIWEQYLLHDNKNITVLTRVADAYRKVRDFRKSKAIYLRVLEM
                     ETDNPYALIGLGHLHYDFKEYKDALQYWQRMVDLQGPAVDIRVLTSIGNCYRKLKQFD
                     RGIPYFEQALDKEPGNFYALFGLADCFRGTGDSAKSLEHWDRILNQDPRNKVILTRAG
                     DAYRALGDYPRATDYYERALNIEFDVYAVLGLAVIARLQGKTDDAIESLRKLIGNDPK
                     NYRLYLELGQCYLAKGDRQKAMDTLADFQRLGIRNPYIQDLVARIQRS"
     gene            complement(3135..3494)
                     /locus_tag="P5313_14795"
     CDS             complement(3135..3494)
                     /locus_tag="P5313_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81680.1"
                     /translation="MTMVKNESDKFSGEGLIKTTDGPRGDLETASKAQLNRRGNELFN
                     AGQIEQARRLFQTTGYSDGLIRVGDWYREHHKPVEALKMYKLAHDDERSGALIERMAL
                     VVQNLLHEEDQDDESIR"
     assembly_gap    3704..3713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3822..4373)
                     /gene="rsmD"
                     /locus_tag="P5313_14800"
     CDS             complement(3822..4373)
                     /gene="rsmD"
                     /locus_tag="P5313_14800"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRY81681.1"
                     /translation="MRITGGYLRGRTISVPKGNLEIRPAMDRMRESVFAVLGDLTGRS
                     FLDLFSGSGILALEAASRGAFPVECVEKDREKFPLLLRNVALSERRIACHRIAAELYL
                     KRTKDRFSVIFLDPPFPYGHRAELLTTISDRGILDPGGLVLLHYPEEDEPPDRCGSLE
                     TTDERKYGRSRVRFYTRRDENAP"
     gene            complement(4405..4869)
                     /gene="smpB"
                     /locus_tag="P5313_14805"
     CDS             complement(4405..4869)
                     /gene="smpB"
                     /locus_tag="P5313_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRY81682.1"
                     /translation="MDGSGTKLIAVNRKARFNYEVEEDLECGIELVGSEVKSFRMGKI
                     SFPDAFAVIEDGEVWIRNLHVAEYVFSSIFNHDPDRKRKLLLHKQEIKRLKRKVDERG
                     YTLVPLSFYFKNGRVKVSLGLCKGKKAADKRAAIKDRDVERDTRRELRERNR"
     gene            5115..5846
                     /gene="lepB"
                     /locus_tag="P5313_14810"
     CDS             5115..5846
                     /gene="lepB"
                     /locus_tag="P5313_14810"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY81683.1"
                     /translation="MRRRSPYSELRIKRLTVKRRLGILALLYLVYEVTTSVFLAPYRV
                     GSLSMSPTLSPGDLVLAFPLAFGPRSALFRKPLGGISDPRRGDLVLLEAPFHERDPWY
                     QEAADSIVRFFTFQQVSLSKSRDRLNSLSIKRVVGIPGDSLYMKGSEVYVKVSGNPHY
                     LTEYEVSGRVYEAAGEGMPEGWPESLPLSGAFPEITLGEGQYFVLGDNRTGALDSRAY
                     GPVDRSRFRARIILRYWPFGSFGIP"
BASE COUNT          953 a         1935 c         1991 g         1118 t
ORIGIN      
        1 ggcccaggtc tccatcaacc tcacggacta ccacgccaca cccctgcacg tcgtctacga
       61 gaccgtccgg gaggaggcgg aaaagctggg tctcctggtg acggggagcg agatcgtggg
      121 gctgcttccc ctggaacccc tcgccgaggc cgggcgcttc tacctgcgcc gcgcgggaaa
      181 gagcgggggg gctccggagg cggagctcct ggagacggcc gtccagtccc tgggcctgga
      241 ttccgtggca cccttcgacg cgaacaagaa ggtcgtcgag tacgcggtgc gctctccgtc
      301 acccctggtt tcccggaccg tggcctcctt cgcggacgag gtgtccgccg attcgcctgc
      361 ccccggcggc ggctccgtgg cggccctggc ggggagcctg ggagcggccc tggccgccat
      421 ggtcgccaac ctgacggtgg gcaagaaggg ctacgaggcg gcctggaagg acctggacgc
      481 cctggccctg cgggcccagg acgtgaaggt ccggctcctg gccgcggtgg acgacgacac
      541 gaacgccttc aacgacgtgc tggcggccca gaagctcccg aagggcacgg aggaggagaa
      601 aacggcgagg gccgccgcga tccaggcggg ctatcgcagc gccaccctgg tgcccctgtc
      661 cacggcccag gcctgcctcg aggccgcccg ggtttccgaa tccgcggcgg tcctgggcaa
      721 cagggcgtcc gtgacggacg ccggagtggg cgccttgctg gccgcggccg gcgccgaggg
      781 ggcggtgctc aacgtgctga tcaacctggg ctccatcgag gatccggaat gggcggccaa
      841 ggtccgtgcg gaggcggaag ccctggctgc ggaagcccga gccgtacggg accggaccct
      901 ggtcaaggtc cgggaggtga tgggcggctg acgccctccg gaaagcggca tcgccgggga
      961 acggaccgat ccgggcctca gtccggggcc cggagggtct ccccgagctg gccgcaggcg
     1021 ccgagcaccc cgcggccccg ccgcatgcgg cgggtgacgg gtattcccgc ggattccagc
     1081 agggtcaccg cctcctccac ggcccgtcgt gagggctccc ggtagggaag gtcgggcacg
     1141 ggattccaag ggatcaagtt gacctggacc ttgagcgggc gggaccattc cacgatggcc
     1201 tccatgtcct ggcgccggtc gttcactccc ccgatcagga ccgcctccag ggtgatccgg
     1261 tcgcccgtct tcgcctggta ataggccagg gcgtcccgga gctccgaaag cccctgggac
     1321 cggttcacgg gcatgagccc gctgcggacc tcgtcgaccg cggaggtcag ggagacggcc
     1381 agacggacgt gggggcccga gtccgcgagg tcccggatcc cctcgggaag gccgcaggtg
     1441 gagatcgtga tcttccggag ggaaagcccc atgccttccg ggtgggacag gatctcgacg
     1501 ctcctgcgga cttccggcag gttcagcaaa ggctccccca tgcccatgaa cacgaggttc
     1561 gagggccggc cgaacatccg ggtcaggtgc aggtactgct ccacgatctc gaaggcctcg
     1621 aggttgcgca ggaagcccag ggtcccggtc ttgcagaagg cgcaggccat ggggcagccg
     1681 acctgggtgg agatgcaggc ggtcttcctc ccctcgatgt ccgtcaagag cacggtctcc
     1741 accgcggcgc cgtccgggag gcggagcctc agcttgacgg tgccgtcctc gtcccggaac
     1801 cggccgtcca cggccgtgcc caggagcgag gggtggagca gggtcaggcg ttccctctcc
     1861 ggtcccggga ggttcgtcat gtcctggaaa gagagggccc ccccggaaag ccacttgaag
     1921 acctggattc cccggaacgg ccggtcgaat ccgcaatcct cggcgatttc atggggcaaa
     1981 aggcccgcaa ggcgtcgggg cacggctagg agcgctgtat cctcgcgacg aggtcctgga
     2041 tgtacgggtt tcgtatccca agacgctgaa agtccgcgag ggtgtccatg gctttctgcc
     2101 ggtccccctt ggccaggtag cattggccca gttcgaggta gaggcggtag tttttcgggt
     2161 cgttcccgat gagcttccgg agggactcga tggcgtcgtc ggtcttcccc tggagtcggg
     2221 ctatgaccgc caggccgagc acggcgtaca cgtcgaactc gatgttcagg gcccgctcgt
     2281 aataatccgt ggcccggggg taatccccga gagcccggta ggcgtccccc gcccgggtca
     2341 ggatgacctt gttccgggga tcctggttca ggatccgatc ccagtgctcc aggctcttgg
     2401 cggagtcgcc ggtcccccgg aagcagtccg caagcccgaa gagggcgtag aagttcccgg
     2461 gctccttgtc cagagcctgc tcgaaatagg ggatcccccg gtcgaactgc ttgagcttgc
     2521 ggtagcagtt cccgatggag gtcaggaccc ggatgtccac cgccggtccc tgcagatcca
     2581 ccatgcgctg ccagtactgg agggcgtcct tgtattcctt gaagtcgtag tgaaggtgcc
     2641 cgagcccgat cagggcgtag ggattgtccg tttccatctc cagaacccgc aggtagatgg
     2701 ccttggattt ccggaaatcc cggactttcc ggtaggcgtc cgccacgcgg gtcaggacgg
     2761 tgatgttctt gttgtcgtgg aggaggtact gttcccagat ctcgatggcc atctggtact
     2821 ggttcaaggc cttgtagcag tccgccaggc cgaagagggc gtagttgttg cccgggtggt
     2881 acaccaggca cttcttgtag gaatccacgg cttcccggta gatgccccgc ttccgggccg
     2941 cgtcgccgat gcctaccagg gcgtagttgt tctccggctc cgtttccagg atcttgcgga
     3001 agcattcctc cgcgtcccgg acgcggtttt ccttgaggta ctggtacccc ttcttcgaca
     3061 gctcggatat ctcgacaagc tcgggcggct gggaaccgga cggggtctcg gcgggatcgg
     3121 ctcccgggat ccgatcatcg gattgattcg tcatcttggt cctcttcatg gagtaggttt
     3181 tgaactacca aggccattct ctcgatcagg gctccgcttc gctcgtcgtc atgggccagc
     3241 ttgtacatct tcagggcttc gacgggcttg tgatgctccc ggtaccagtc gccgacccgg
     3301 atcagcccgt ccgagtatcc cgtggtctgg aacaaccgcc tcgcctgctc gatctgtccc
     3361 gcgttgaaca gttcgtttcc acggcggttg agctgggcct tgctggcggt ttccaggtcc
     3421 ccccggggcc cgtccgtggt cttgatgagg ccttcaccgg agaacttgtc ggactcgttt
     3481 tttaccatag tcattcccga ctatactaaa aaaaacctag gaatcaacat cgcaccgttg
     3541 atgtccaatt tatccagaaa ttccgaaacg accgtggtat ggttctctat cgagagcctg
     3601 cctccctgga ggaaccgatg atggaagaac cggatcgcgg cgcggaaacc ttgaagctgg
     3661 tcgaagattc catcgtgccg gggttcaccg gagagtacgt attnnnnnnn nnnccccggg
     3721 ccggtttcgg gcaggctcca cggcgacggc ccgtccccgg atgcccgggg ccggcgtctg
     3781 cgatcctatc tccgtcgatt ggatcggtaa acgtcgtccg gtcagggggc gttttcgtcc
     3841 cggcgggtgt agaaccggac ccgcgatcgg ccgtatttcc gctcgtccgt ggtttccagg
     3901 ctgccgcagc gatccggggg ttcgtcctcc tcggggtaat ggagcaggac gagaccgccg
     3961 ggatccagga ttcccctatc ggaaatcgtc gtcagaagtt ccgcccggtg gccgtagggg
     4021 aagggcggat ccaggaatat caccgaaaac cggtccttgg tccgcttcag gtacagttcc
     4081 gcggctatcc ggtggcaggc gatgcgccgt tccgaaaggg ccacgttccg gaggagcagg
     4141 gggaatttct cccggtcctt ctccacgcac tcgaccggga acgccccgcg gctggcggct
     4201 tccagggcca ggatgccgga gcccgagaac aggtccagga aggatcggcc ggtcaggtct
     4261 cccaggaccg cgaagaccga ttcccgcatc cggtccatgg cgggccggat ctccaggttc
     4321 cccttgggca cggaaatcgt tcttccccgc aggtatcccc cggttatccg cacgaagccc
     4381 tcccggggcc gacacggagg aggctcaccg gttccgctcc cggagttccc gccgggtatc
     4441 ccgttccacg tctcggtcct tgattgcggc ccgcttgtcc gcggccttct ttcccttgca
     4501 cagccccagg ctgaccttca cacggccgtt cttgaagtag aaggacaggg gcaccagggt
     4561 atagccccgc tcgtccacct tgcgcttgag gcgcttgatc tcctgcttgt gcaggaggag
     4621 ctttcgcttc cggtccgggt cgtggttgaa gatggaggaa aagacgtatt ccgccacgtg
     4681 gaggttgcgg atccagacct cgccgtcctc gatcaccgcg aaggcgtcgg gaaaggagat
     4741 cttacccata cggaaggact tcacctcgct gcccacgagt tcgatgccgc actcaaggtc
     4801 ctcctcgacc tcgtagttga accgggcctt gcggttgacg gcgatcagtt tcgtgccgga
     4861 tccgtccata tcaggctcca ggtcccgggg cgcagcggcg cggtccgggt tcgcgggtcg
     4921 ggccgagacg ggacggcggg gagacggcgg cgggtccttt ttcggcttct cggttgagtc
     4981 tcggcatggt ggtggattat aggaagccgc cggaagcctt gtcaacgcgg gccaaagatg
     5041 ctaccgttcc gcccgggtat ccagttcatc catcggcccc cggaccggcc ccggggcgat
     5101 acggcaggga gtgcatgcgt agaaggtccc cgtattcgga gctgcggatc aagcgcctta
     5161 cggtgaagcg acgcctgggc atccttgcgc tcctgtacct cgtctacgag gtcaccacta
     5221 gcgtgttcct ggctccgtac cgggtgggat ccctgtccat gtcgccgacc ctatcacccg
     5281 gggacctcgt cctggccttt cccctggcct tcggtccccg gtcggccctg ttccgcaaac
     5341 ccttgggggg tatctcggat ccccgacgcg gggacttagt gctcctggag gctcccttcc
     5401 acgagcgcga tccctggtat caagaagcgg cggactccat cgtgcgattt ttcaccttcc
     5461 agcaagtctc cctgtccaag tcccgggatc gattgaacag cctgtccatc aagcgcgtcg
     5521 tgggcatacc gggggattcc ctgtacatga agggcagcga ggtctatgtg aaggtttccg
     5581 gcaatcccca ctacctgacg gagtacgagg tttccggacg ggtctacgaa gccgccggcg
     5641 aaggcatgcc ggagggctgg cccgaatccc tgcccctctc gggcgccttc ccggagatca
     5701 ccctggggga ggggcagtat ttcgtcctgg gggacaaccg taccggcgcc ctggattccc
     5761 gggcttacgg ccccgtggac cggagccggt tccgggcccg gatcatcctg cggtactggc
     5821 ccttcggatc cttcggaatc ccttgaattc cgcggaaatc ctgccggacc tgcccggtct
     5881 ctacgtgcac gtcccctact gcgcggggaa atgcgggtac tgttccttcc attcgcggcc
     5941 cctgaaagcc tgtcccgacg acgaaccgga gaagatgacg tcggtcctgc tggaccgggc
     6001 gcgggac
//
LOCUS       DAOTEI010000200         5899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_9661_length_5899_cov_4.552190, whole genome shotgun sequence.
ACCESSION   DAOTEI010000200 DAOTEI010000000
VERSION     DAOTEI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9661_length_5899_cov_4.552190"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="P5313_14815"
     CDS             complement(<1..644)
                     /locus_tag="P5313_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81684.1"
                     /translation="MRPRYRALAPIFLVLLAAAASAAQDAVPQPEPQPPAPAPAAPAA
                     PAAPVPAPSFSPAPAPIPSRVDALELYRQGRNLESAGRTAEAQARYREAVAACDRELA
                     ADPARMDAYAVKSWSLFRLDRFREVVDTATAGLKVRYDPRLSEVMGEAYFHLGNNELA
                     LKNLQRYIENVGEFGDRVSTAYFYMGEVYQRLKKFDHADIAYALAVHRDPSMSR"
     gene            complement(699..2189)
                     /gene="gltA"
                     /locus_tag="P5313_14820"
     CDS             complement(699..2189)
                     /gene="gltA"
                     /locus_tag="P5313_14820"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRY81685.1"
                     /translation="MIRTMEQLASEARAVWADISSRELKPKDRLAIPVQDMPCQDPVE
                     RGRNMNEVALGYTEEQARVEASRCIQCKNAPCIAGCPVRIDIPGFIGKVRDGDYASAA
                     AIIKRTNLLPSICGRVCPQEVQCQLPCTLGKSLGGVDKAVQIGRLERFVADREARDGT
                     ARAPEIAPDTGKKVAVIGSGPAGLTVAADVRRAGHTVTIFEAFQKPGGVMVYGIPEFR
                     LPKAIVQSEAKALEALGVEFKLNFLVGRTRPLMSLLSEDGFDAAFVGVGAGLPKFMEI
                     PGENLVGVFSANEYLTRANLMKAYDRSRAYTPIYPSRRVAVLGGGNVAMDSARMALRL
                     GAEEVRVLYRRTREEMPARAEEVEHAMEEGIHFDFLSSPVEILADSEGKAAGLRVQAF
                     RLGDPDSSGRRSPVAIPGAITEVPFDTVIVAIGNESNPLLARTTPGLVVDKKGRVIVD
                     EAQKSSVDRVYAGGDIVLGAATVILAMGEGRKAAASINGILSNHQG"
     gene            complement(2186..3070)
                     /locus_tag="P5313_14825"
     CDS             complement(2186..3070)
                     /locus_tag="P5313_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRY81686.1"
                     /translation="MNRITAKRLLSEDVYRIEVEAPDIARERKAGQFVIVQADTEWGE
                     RIPLTIADADPGKGSIVLVFQAVGASTHKLARKEPGDEIAVLGPLGNPTHIENFGHAV
                     CVGGGIGVAPLHPIVQALKAAGNRLTVIMGARTKSLLIMEEEMRALADDLVVVTDDGS
                     YGRKALVTEPLKELCQREPKPDLAVAIGPPVMMKFCAETTRPFGVHTVVSLNTIMIDG
                     TGMCGGCRVSVGGETKFVCVDGPEFDGHKVDFDQMMRRLRAYKSIEDAAHERCHLELA
                     AEELAGKKAAEAAKGGAR"
     gene            complement(3067..4980)
                     /locus_tag="P5313_14830"
     CDS             complement(3067..4980)
                     /locus_tag="P5313_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HRY81687.1"
                     /translation="MNKLRFKAITRMVSISLGAALLMRAFLALYLARDAASVLDGLGS
                     FAAIVGILLAASALWIYALMGPLVRAAAKGAYGTPLSPEEKETAAAAGYRIPTAVTVI
                     LVLAFLIGPVISMGMNALSGTARYTATETVLIVLLNLSFGLMARTHITESLEGILSEA
                     VRALGIHALPPGARKRGLRNRLVLAGLSGVFLALFLLAVSGLGYIRSLSRGFQNPEAF
                     QTRYAAETGIVVVLVLAWTAWVIARLARNVASDIGSVTGQILTIAEGRGDLSRKADIH
                     RTDEIGFLVEAFNRFLAGLEALVGKARASAEAVRASSESLAATADEATQAVGNMEGSL
                     ENMRAAVDRQNAEIGDTEGTIARMIESIDEVAAQVSTQAGFVEQSSAAVAEMAANIGS
                     VSSVADRADGVARKLKEAADEGDSALADSMKAIQEIDESARSVRETVLVLSKIAAQTN
                     LLAMNAAIEAAHAGEAGKGFAVVAAEVRSLAENAAASAKNIAALMKEMNGKVERGSAL
                     AGKAGEAFMSIRQGVEQTTELVRTVSSSMAEQKSGAQEILGSVNSLIEATTAIRDLTA
                     EQKDKSKGMEQAMLRIVEASNNIFEAVQEETGSTQALSRVVRTVREEAENNRGHVGDL
                     DAAVSKFKVGEGR"
     gene            5265..>5899
                     /locus_tag="P5313_14835"
     CDS             5265..>5899
                     /locus_tag="P5313_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRY81688.1"
                     /translation="MPTKKNLVMIDGNTAAAHVAHACSEVVAIYPITPSSNMGELADE
                     FSAQGRKNIFGTVPQVVELQSEAGAAGAVHGSLTTGALTTTFTASQGLLLMIPNMFKI
                     AGEATPAVFHIAARAVAAHALSIFGDHQDVMAARQTGWALLGSANVQEAMDLALVAHA
                     ATLEARVPFLHFFDGFRTSHEVQKVEELSQDVMRKMIDSKFVRAHRARGLNP"
BASE COUNT          888 a         2070 c         1979 g          962 t
ORIGIN      
        1 cagcgggaca tggaggggtc ccggtgcacc gccagggcgt aggcgatgtc cgcgtggtcg
       61 aacttcttga gacgctggta gacctcgccc atgtagaaat aggccgtgga cacccggtct
      121 ccgaattccc cgacgttctc gatgtacctt tgcaggttct tcagggccag ctcgttgttg
      181 cccaggtgga agtaggcctc ccccatcacc tcggacagcc ggggatcgta gcggaccttc
      241 aggcccgccg tggccgtgtc cacgacctcc cggaaccggt ccaggcggaa gagggaccag
      301 ctcttgaccg cgtaggcgtc catgcgggcc ggatcggcgg cgagctcccg gtcgcaggcg
      361 gccacggcct cccggtaccg cgcctgggct tccgcggtcc gtcccgcgga ttcaaggttc
      421 cggccctggc gatagagctc cagggcgtcc acccgggaag ggatcggagc cggagccggc
      481 gagaaggacg gggcgggaac cggcgcggcc ggagccgcgg gcgcggcggg cgccggcgcg
      541 ggaggctggg gctccggctg cgggacggca tcctgggcgg ccgaggcggc cgcggccagg
      601 aggaccagga agatcggggc tagggctcgg tatctggggc gcatggaatc ctccggactg
      661 cgccccgatt atcccttaaa tgcccccgcc gcggcaagct agccctgatg gttcgacagg
      721 atcccgttga tggatgccgc ggccttgcgg ccctctccca tggccaggat gaccgtggcg
      781 gcccccagga cgatgtcgcc ccccgcgtag acccggtcca ccgagctctt ctgcgcctcg
      841 tccacgatga cccggccttt cttgtccacc accagcccgg gggtcgtgcg cgccaggagg
      901 gggttggact cgttgccgat ggccacgatg acggtgtcga agggcacctc ggtgatcgct
      961 ccggggatgg ccacggggga acggcgtccg gaggaatccg ggtcacccag gcggaaggcc
     1021 tggacccgga gccccgcggc cttcccctcg gagtccgcca ggatctccac ggggctggag
     1081 aggaagtcga aatgaatccc ctcctccatg gcgtgctcca cctcctcggc ccgggcgggc
     1141 atctcctccc gtgtgcggcg gtagagcacc cggacctcct cggcccccag gcgcagggcc
     1201 atgcgggcgg agtccatggc cacgttgccg ccccccagga cggccacgcg gcgggagggg
     1261 tagatggggg tgtaggcccg gctccggtcg taggccttca tcaggttggc ccgggtcagg
     1321 tactcgttgg cgctgaacac ccccaccagg ttctccccgg ggatttccat gaacttggga
     1381 agtcccgccc ccacccccac gaaggccgcg tcgaagccgt cctcggacag cagggacatg
     1441 aggggccggg tgcggccgac caggaagttg agcttgaact cgacccccag ggcctccagg
     1501 gccttggcct cggactggac gatggccttg ggcagccgga actcggggat cccgtagacc
     1561 atgaccccgc ccggcttctg gaaagcctcg aagatggtga ccgtatggcc cgccctgcgc
     1621 acgtccgcgg ccacggtcag gcccgcgggt cctgagccga tcacggccac tttcttgccc
     1681 gtgtccgggg cgatctcggg agcccgggcc gttccatccc gggcctcccg gtccgccacg
     1741 aagcgctcca ggcgcccgat ctgcaccgcc ttgtccaccc cgcccaggga cttgcccagg
     1801 gtgcagggaa gctggcactg gacctcctgg ggacagacgc ggccgcagat ggagggcagg
     1861 aggttggtcc gtttgatgat ggccgcggcg gaggcgtagt ccccgtcccg gaccttgccg
     1921 atgaagccgg ggatgtcgat ccgcaccggg cagcccgcga tgcagggggc gttcttgcac
     1981 tggatgcacc gggaggcctc cacccgggcc tgctcctccg tgtaacccag ggccacttcg
     2041 ttcatgttgc ggccccgctc cacgggatcc tggcagggca tgtcctggac ggggatggcc
     2101 aggcggtctt tgggcttcag ctcccggctc gagatgtcgg cccagacggc ccgggcctcg
     2161 gaggcgagct gttccatggt gcggatcatc gagcgcctcc cttcgcggcc tcggcggcct
     2221 ttttcccggc cagctcctcg gcggccagct ccaggtggca cctctcgtgg gcggcgtcct
     2281 cgatggactt gtaggccctt aggcggcgca tcatctggtc gaagtccacc ttgtggccgt
     2341 cgaactccgg tccgtccacg cagacgaact tggtctcgcc gcccacggat acccggcagc
     2401 ccccgcacat cccggtcccg tcgatcatga tggtgttcag ggacacgacg gtatgcactc
     2461 cgaaggggcg ggtggtctcg gcgcagaact tcatcatgac gggcggcccg atggccaccg
     2521 ccaggtcggg cttgggctcc cgctggcaga gctccttgag gggctccgtg accagggcct
     2581 tgcggccgta ggatccgtcg tcggtcacca cgacgaggtc gtccgccagg gcccgcatct
     2641 cctcctccat gatgaggagg ctcttggtgc gggcgcccat gatgacggtc aggcggttcc
     2701 ccgcggcctt cagggcctgg acgatcgggt ggaggggcgc cacgccgatc cctccgccca
     2761 cgcagaccgc gtggccgaag ttctcgatgt gggtcgggtt gcccaggggc cccaggacgg
     2821 cgatctcgtc ccccggctcc ttccgggcca gcttgtgggt ggacgccccc acggcttgga
     2881 acaccaggac gatggagccc ttcccgggat ccgcgtccgc gatggtcagg gggatgcgct
     2941 ccccccactc cgtgtcggcc tgcacgatga cgaactgccc ggccttgcgc tcccgggcga
     3001 tgtcgggcgc ctccacctcg atgcggtaca cgtcctccga caggaggcgc tttgcggtga
     3061 tgcgattcat cttccctctc cgaccttgaa tttggatacc gcggcgtcga ggtcccccac
     3121 gtggccgcgg ttgttttccg cctcttcccg taccgtgcgc accacccggg acagggcctg
     3181 ggtgctcccc gtctcctcct ggaccgcctc gaagatgttg ttcgaggcct ccacgatgcg
     3241 cagcatggcc tgctccatgc ccttggactt gtccttctgc tccgcggtca ggtcccggat
     3301 ggccgtggtg gcctcgatca gggagttgac gctgcccagg atctcctggg ccccggactt
     3361 ctgctccgcc atggaggagg agacggtccg caccagctcc gtggtctgct ccaccccctg
     3421 ccggatgctc atgaaggcct cgccggcctt gcccgccagg gcggagcccc gctccacctt
     3481 gccgttcatc tccttcatga gggccgcgat gttcttggcg ctggccgcgg cgttctccgc
     3541 cagggaccgc acctccgcgg ccaccaccgc gaagcccttc ccggcctcgc ccgcgtgggc
     3601 ggcctcgatg gccgcgttca tggccagcag gttggtctgg gccgcaatct tggacaggac
     3661 caggaccgtc tcccggaccg agcgtgcgga ctcgtcgatc tcctggatgg ccttcatgga
     3721 gtccgccagg gcagagtcac cctcgtccgc cgcctccttg agcttccggg ccaccccgtc
     3781 cgcccggtcc gccacggagg agaccgagcc gatgttggcg gccatctcgg ccaccgccgc
     3841 ggagctctgc tccacgaagc ccgcctgggt gctgacctgg gcggccacct cgtcgatgga
     3901 ctcgatcatg cgggcaatgg tgccctcggt gtccccgatc tcggcgttct gccgatccac
     3961 ggcggcccgc atgttctcca gggagccctc catgttgccc accgcctggg tggcctcgtc
     4021 ggcggtggcc gccagggact ccgaggaggc gcgcaccgcc tcggcggagg cgcgggcctt
     4081 gccgaccagg gcttcgaggc ccgccaggaa gcggttgaag gcctccacca ggaagccgat
     4141 ctcgtcggtg cggtggatgt cggcctttcg gctcaggtct ccccgtccct ccgcgatggt
     4201 caggatctgc cctgtcacgg acccgatgtc cgaggcgacg ttccgggcca gccgggcgat
     4261 gacccaggcg gtccaggcca ggaccaggac cacgacgatg cccgtctccg cggcgtaccg
     4321 ggtttggaag gcctcggggt tctggaagcc ccgggaaagg ctccggatgt agcccaggcc
     4381 ggataccgcc aggaggaaga gggccaggaa gacccccgag agcccggcca ggaccaggcg
     4441 gttccggagc ccccgtttcc gggcccccgg gggcagggcg tggatgccca gggccctgac
     4501 cgcctcggaa aggattccct ccagggactc ggtgatgtgg gtgcgggcca tgagcccgaa
     4561 ggagagattg aggagcacga tcaggaccgt ctccgtcgcc gtataccggg cggtcccgga
     4621 gagggcgttc atacccatgg agatgacggg gccgatgagg aaggccagga ccaggatgac
     4681 cgtgaccgcc gtggggatgc ggtagccggc cgcggcggcg gtctccttct cctccgggga
     4741 caggggggtc ccgtaggcgc ccttggcggc ggcccggacc aggggtccca tgagggcgta
     4801 gatccagagg gccgaggcgg cgagcaggat tcccacgatc gcggcgaagg aacccaggcc
     4861 gtccagcacg gaagccgcgt cccgggccag gtagagcgcc aggaaggccc tcatgaggag
     4921 ggccgcgccg aggctgatgg aaaccatgcg ggtgatggcc ttgaacctca gtttgttcat
     4981 ggaagccctc cgggtacgac cgatggaacg tcctccggca aaaacccgga ggcaccccat
     5041 actacaacct aagcggacgg atgcaaaggg ctcgctctgc ccgctcctcc gggcgtccga
     5101 gagtccattt cgggcgtgat cgtcaaatcg tataaaatta tagatacgga tttatcattt
     5161 gacaagcaga ttccaagatc tccgcttgat ataaatcccg aaagggcata ctataagtgg
     5221 gtccggcggt tgccggcccc acacccctac tctgaggatc caacatgccc accaagaaga
     5281 atctggtcat gatcgacggc aataccgccg ccgcccatgt agcccacgct tgttccgagg
     5341 tggtggcgat ctacccgatc accccctcct ccaacatggg agagctcgcg gacgaattct
     5401 ccgcccaggg acgcaagaac atcttcggta ccgtccccca ggtcgtggaa ctccagtccg
     5461 aagccggagc cgcgggggcc gtccacggtt ccctgaccac cggagccctg accaccacct
     5521 tcaccgccag ccagggcctg ctcctcatga tcccgaacat gttcaagatc gccggcgagg
     5581 ccacccccgc tgtcttccat atcgcagccc gagccgtggc agcccatgcc ctgtccatct
     5641 tcggggatca ccaggacgtc atggccgccc gccagaccgg ctgggccctc ctcggatccg
     5701 ccaacgtcca ggaggccatg gacctggccc tggtggccca cgccgccacc ctggaggccc
     5761 gggttccctt cctgcacttc ttcgacggct tccgcacttc ccacgaagtc cagaaggtgg
     5821 aggagctctc ccaggacgtg atgcggaaga tgatcgactc caagttcgtc cgcgcccacc
     5881 gcgcccgggg cctcaaccc
//
LOCUS       DAOTEI010000201         5743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10031_length_5743_cov_4.673172, whole genome shotgun sequence.
ACCESSION   DAOTEI010000201 DAOTEI010000000
VERSION     DAOTEI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10031_length_5743_cov_4.673172"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5313_14840"
     CDS             complement(<1..304)
                     /locus_tag="P5313_14840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY81689.1"
                     /translation="MSRKAGIVGYGLSGRTFHLPLIRSLPEDFEVSAVAAGRRFEEAA
                     RELPEARIVRTAEELFRLPGLDLAVIASPSGLHAAHARAALEAGLAVVLEKPFASTV"
     gene            complement(301..1896)
                     /locus_tag="P5313_14845"
     CDS             complement(301..1896)
                     /locus_tag="P5313_14845"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRY81690.1"
                     /translation="MPEESPPRVAEAARGGFSVEYRGRCLYSRVAPREGPERIVRDLE
                     PLPETLYVVPSPLLGYGLPSLLDKIPESSAVLALEADPVLAAFSRSRLPPEVTGHPRF
                     RFLACPFPADGSLGFPGGFRRASEVRLSAGRALHPEAYDAALRTVDAELNRYWRNRMT
                     LVRMGRLWIRNIFRNLARLPAAGLREPADWTGPVAVCGAGPSLDGACDWLRSERGRLR
                     ILACDTALGPLVLRGIRPDAVVCLEGQIHNLKDFLPAGKMEIPVFVDLSSHPSVFHAV
                     PGPKILTLTRFEDLGLLDRLERLPLPALPVPPLGSVGVLAVHLARRLTDGPVFLTGLD
                     FSFPAGTTHAKGAPAPRADLLREDRFHKARGQWSASWPTGGSRAAGGAGIIMETYAAL
                     LAEEVRGDSRVFDIRDSGLPLGLDRIPLKEASALLDDPRIGAAASGPLPLGRSVPRED
                     LRSAAESFLEVEADRLEGLLSALGGRGDLAARVSEFDWIHSWFPDGERVRELGPDVRN
                     RLLAEALDWKRRIREARTGLEGS"
     gene            complement(1896..3431)
                     /locus_tag="P5313_14850"
     CDS             complement(1896..3431)
                     /locus_tag="P5313_14850"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRY81691.1"
                     /translation="MSSPKQKEPTAAEGRKPAKEKPASNRNTFVYIGTIVILVIVVVA
                     FVFVPSFGGPSADGRLPDFGSYAGTPIRYEQGGYMAGRVQAINDQLKSYGYDQAGNQF
                     FAFQVWKGAFDQTVIHTALLHEARKAGITVSEDRLNEAVLEAPEFLDNGKFSLERYRE
                     ASESRKLAIRTSLQESLLKNHVLEDALSLRPSSREIDFIQAMAREERVVEYVLLPFSD
                     FPDERKAAFGRENPSKFRKINLSRITLNDEKTAGSVLAKVKAGTQTFEEAAKADSKDA
                     WAEQGGASGPRWGYELESELAAAQDAQTLLSLKAGEISPAIKTASGSWAFFRAESPAS
                     EPDFDSPDTLKAVWDYMNKWERGRIEDAAVLRGKDLAVDAAARGFVSAAGSYGLTVRE
                     STPFPLAYGDAFSSGSTSLFRKIAGEGLEGASTNEKFLTAAFSLPAGAVSEPLLLQNA
                     AVVLRVKEIRPAEDTGVQLIEFYYPYLFAQAREMELYDRFLASPELKDNFSTAFFQIF
                     RTE"
     gene            complement(3594..>5743)
                     /locus_tag="P5313_14855"
     CDS             complement(3594..>5743)
                     /locus_tag="P5313_14855"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY81692.1"
                     /translation="IDGLNESAWNGMFENFDAALVQAREALGESDKIGYPEGMAYAQL
                     NIGWCHSYLGGNEEARAAFERALELYRSLDDPAGRSKALNGLGVLYHNMSRYERALDY
                     YTQSLDEARRHGFRDREATTLNNIGEVCADLGNYKEALDYFLKAYDIVPEEKGSELKA
                     GILLNIGKSFTRLENYDLAEEFVRKALALAEPESERVIIASAWDVLGRISRGLGNPSA
                     AEERFRKAIALAEGSKSRREIIEALLDLGSLLVDRGSLEEAQEVLERAARTSEEIHAK
                     ALYYAAYERLAETHERKGDHRTALDYFRRFVRYEREILNEDTTRKIKDVQIQYEVERT
                     QREAEIYRLRNIELKEKTESLEEMNRQILTISEIGQRITSSLDMETVVSTLYESLRAL
                     LVTDLFGIALYDKPSECLRYVAYIQDGRRIPRDPVPLDPKRSFAAWCIRHDQPVFIRD
                     LNAEYRKYLQGEPMFFLGKPGLSFLYLPLSIEKKVIGVLTVQSYSKESYTDQHLRFLT
                     ALAPFAAIAVENALIHVRVEELNRELQGEKEQLERATHQISYLANHDSLTGLPNRRLL
                     FELLRKSFDLARRSGSKVGIIYVDLDDFKPINDKFGHLAGDRALVEISERLQSVLRAS
                     DTVARVGGDEFVAVLNHVRGKGDVESAARKVLEVCDAPLEFLGESCRIGASMGIAMFP
                     DDGDSLEALVHCADSAMYTIKHSSKKGYAFYSR"
BASE COUNT          939 a         1865 c         1956 g          983 t
ORIGIN      
        1 ggacggtcga ggcgaagggc ttttccagga ccaccgccag gccggcctcc agggccgccc
       61 gggcatgggc cgcgtggaga ccgctcgggg acgcgatcac ggcgaggtcc aggccgggga
      121 gtcggaagag ttcctccgcg gtccggacga tcctggcttc cggaagctcg cgtgcggcct
      181 cctcgaaccg tctccccgcg gccacggcgg atacctcgaa gtcctccggc agggaccgga
      241 tcaggggcag atggaaggtc cttccggaaa gtccgtagcc gactataccc gccttgcggc
      301 tcatgatccc tccagtccgg tccgggcttc ccggatcctg cgtttccaat ccaaagcttc
      361 cgccagcagg cggttgcgga cgtccgggcc cagctctcgt actcgttccc cgtcgggaaa
      421 ccaggaatgg atccagtcga attccgatac ccgggccgcg aggtcaccgc gaccgcccag
      481 ggcggacagc agaccttcca gcctgtccgc ctctacttcc aggaaggatt ccgcggcgga
      541 ccgcagatcc tcccggggaa cggatcggcc cagggggagc ggaccggaag ccgccgcgcc
      601 gatccgaggg tcgtccagga gggcggaagc ttccttcaag ggtatccgat ccaggccgag
      661 gggcaggccg gagtcccgga tgtcgaagac tctcgaatcc ccccggactt cctcggccag
      721 gagcgccgcg taggtttcca tgataattcc ggctcccccg gcggcgcggg atccccccgt
      781 cggccaggag gcgctccatt ggccccgggc cttgtggaac cggtcttccc ggaggaggtc
      841 ggcccggggg gctggggccc ccttggcgtg ggtggtcccc gcgggaaagg aaaaatccag
      901 gccggtcagg aagacggggc cgtccgtcag acgccgggcc aggtgtacgg ccagcacacc
      961 cacggacccc aggggcggaa ccgggagtgc ggggagggga aggcgttcca agcggtcgag
     1021 aagtcccagg tcctcgaacc gggtaagggt caggatcttg gggcccggga ccgcgtggaa
     1081 gacggaggga tgggaggaaa gatccacgaa caccgggatc tccatctttc cggcggggag
     1141 aaagtccttc aggttgtgga tctggccctc caggcagacg accgcgtcgg gccggatccc
     1201 cctcagtacc aggggtccca gggcggtgtc gcaggccaag atacggagcc gacccctctc
     1261 ggaccgcagc cagtcgcagg ccccgtccag gctcggcccc gcgccgcaga ccgccacggg
     1321 acccgtccag tcggcgggtt cccgcagccc ggcggccgga aggcgggcca ggttgcgaaa
     1381 gatgttccgg atccagagtc gtcccatccg caccagggtc atccggttcc gccagtatcg
     1441 gttcagttcc gcgtccaccg tccggagcgc tgcgtcatac gcctccggat gcagggcccg
     1501 gcccgcagac aaccggacct ccgaagccct ccggaaaccg cccgggaaac cgagggatcc
     1561 gtcggccggg aacgggcagg cgaggaaccg gaatctcggg tgccccgtca cctcgggggg
     1621 aagtctcgaa cgggagaagg ctgccaggac cggatccgcc tccaaggcca ggactgcgct
     1681 cgactcgggt atcttgtcca gcagggaggg aaggccgtag ccgagaaggg gggaggggac
     1741 gacgtagagg gtttcgggga gaggttccag gtcccggacg atccgttcgg ggccttcccg
     1801 gggcgccacg cgggaataca ggcagcgccc ccggtattcc acggaaaagc ctccccgggc
     1861 cgcttccgcg acgcggggag gcgattcctc gggcatcact ccgtccggaa gatctggaag
     1921 aaggcggtcg agaagttgtc cttcagttcc ggactggcca ggaacctgtc gtagagctcc
     1981 atctcccggg cctgggcgaa gaggtagggg tagtagaact cgatcagctg tactcccgtg
     2041 tcctccgccg gccggatctc cttgacccgg agcaccacgg cggcgttctg caagagaagg
     2101 ggctccgaca cggccccggc gggcagggag aaggccgcgg tcaggaactt ctcgttggtc
     2161 gaggctccct ccagcccctc gccggcgatc ttccggaaca ggctcgtgga gccgctggag
     2221 aaggcgtccc cgtacgcgag ggggaagggc gtcgattccc ggacggtcag gccgtacgaa
     2281 ccggcggcgg agacgaagcc ccgggccgcg gcgtccacgg cgaggtcttt gccccgcagg
     2341 accgccgcgt cctcgatccg gccgcgttcc cacttgttca tgtagtccca aacggccttc
     2401 aaggtatccg gggagtcgaa gtcgggttcc gaggccgggc tttcggcccg gaagaaggcc
     2461 caggatcccg aggcggtctt gatcgccggg gagatctccc cggctttcaa ggaaaggagg
     2521 gtctgggcgt cctgggccgc ggccaactcg gattccagct cataccccca acgcgggccc
     2581 gaagccccgc cctgctcggc ccacgcgtcc ttggaatcgg ccttggcggc ctcctcgaag
     2641 gtctgggtcc ccgccttgac cttggccagg acggagccgg ccgtcttctc gtcgttcagg
     2701 gtgatccggg agagattgat cttgcggaac ttggagggat tctcccgacc gaaggcggcc
     2761 ttccgctcat ccgggaagtc ggagaagggc aggagcacgt actcgacgac ccgttcctcc
     2821 cgggccatgg cctggatgaa gtcgatctcc cgggaactgg gccgcaggga cagggcgtcc
     2881 tccaggacat ggttcttgag cagggactcc tgcagggagg tgcggatggc cagcttcctg
     2941 gattccgagg cctcgcgata ccgttccagg gagaacttgc cgttatccag gaactcgggg
     3001 gcttccagga cggcttcgtt caggcggtcc tcggagacgg tgatccccgc cttccgggct
     3061 tcatgcagga gggccgtatg gatcacggtc tggtcgaacg ctcccttcca gacctggaag
     3121 gcgaagaact ggttgcccgc ctggtcgtag ccgtaggact tgagctggtc gttgatggct
     3181 tggacccggc cggccatgta cccgccctgt tcgtaccgga tgggggtgcc cgcgtaactg
     3241 ccgaaatccg gcagcctgcc gtccgcggag gggccgccga aggacggcac gaacacgaag
     3301 gcgaccacca cgatcaccag gatgacgatg gttccgatgt agacgaaggt gttgcggttc
     3361 gaggcgggtt tttccttggc cggtttgcgg ccttccgcgg cggtcggttc tttctgcttg
     3421 ggagacgaca tggcttcctt ccctagatgt gatcgtggat cgtttcccgc ggcgccttga
     3481 aacggaggaa gggtatggga gatccatccc ggagatggag gccgcagcgg ggccgaagca
     3541 aaataccacg tcccccgggc ctcgtcaata cgcggcgatc cggggctccg tccctaccgc
     3601 gaatagaacg cgtaaccctt tttggaagag tgctttatcg tgtacatggc ggagtccgcg
     3661 cagtggacca gggcctccag ggagtccccg tcgtccggga acatggcgat gcccatggac
     3721 gccccgatcc tgcaggattc ccccaggaat tccaggggcg cgtcgcagac ctccaggacc
     3781 ttccgggcgg cggactccac gtcccccttg ccgcggacat ggttcaggac agccacgaac
     3841 tcgtccccgc ccacccgggc cacggtgtcc gaggcccgca gcacggactg caggcgctcg
     3901 gaaatctcca ccagggcccg gtcgcccgcc aagtgtccga acttgtcgtt gatgggcttg
     3961 aagtcgtcca ggtccacgta gatgatcccc accttggagc ctgaccgccg ggccaggtca
     4021 aagctcttcc gcagcagttc gaagaggagc cggcggttcg gcagccccgt gaggctgtcg
     4081 tggttggcca ggtagctgat ttggtgggtg gcccgttcca gctgctcctt ttccccctgg
     4141 agttcacggt tcagttcctc cacccggaca tggatgaggg cgttctccac cgcgatggcg
     4201 gcgaagggcg ccaaggccgt caggaaccgc agatgctggt ccgtgtagga ttccttggaa
     4261 tagctctgga ccgtcagcac cccgatgacc ttcttttcga tcgaaagggg gaggtagagg
     4321 aaggaaagtc ccggcttgcc caggaagaac atcggctccc cctgcagata cttccggtat
     4381 tccgcgttca ggtcccggat gaagacgggc tggtcgtgcc ggatgcacca ggccgcgaag
     4441 ctccgcttgg gatccagggg gacggggtcc cgtggaatcc gccggccgtc ctggatgtag
     4501 gccacgtagc ggaggcactc cgaaggcttg tcgtacaggg cgatgccgaa gaggtccgtc
     4561 accaggagcg cgcggaggct ttcgtagagg gtggatacca cggtttccat gtccagactg
     4621 gaggtgatcc gctggccgat ctcggagatc gtcaggatct gccggttcat ctcctccagg
     4681 ctttccgtct tctccttcag ctctatgttg cggagccgat agatctcggc ctcccgctgg
     4741 gtccgttcca cctcgtactg gatctggacg tccttgatct tccgggttgt gtcctcgttc
     4801 aggatttccc gttcgtaccg gacgaaccgg cggaagtagt ccagagcggt ccggtggtct
     4861 cccttccgct cgtgggtttc cgcgagacgc tcgtaggcgg cgtagtacag ggccttggcg
     4921 tggatctcct cgctggtccg ggccgcccgc tccaggacct cctgggcctc ctcgaggctt
     4981 ccccggtcga cgaggaggga gccgagatcc aggagggcct cgatgatctc cctccggctt
     5041 ttggacccct ccgccagggc gatggccttg cggaaccgct cctccgccgc cgagggattg
     5101 cccaggcccc gggagatcct acccagcacg tcccaggcgg aggcgatgat cacccgttcg
     5161 ctctcgggtt ccgccagggc cagggccttg cggacgaact cctccgccag gtcgtaattc
     5221 tccaaccggg tgaaggactt cccgatgttg agcaggatgc cggccttgag ctcggagccc
     5281 ttctcctcgg gcacgatgtc gtaggccttc aggaagtagt ccagggcttc cttgtagttg
     5341 cccaggtcgg cgcagacctc cccgatgttg ttcagggtcg tggcctctcg gtcgcggaac
     5401 ccgtgccgac gggcctcgtc caggctctgg gtgtagtagt ccaaggcgcg ctcgtaccgg
     5461 gacatgttgt ggtacagcac acccaagccg ttcaaggcct ttgagcggcc cgcggggtcg
     5521 tcgaggctgc ggtacagctc cagggcgcgc tcgaaggcgg cccgggcttc ctcgttgccg
     5581 cccaggtagg agtggcacca tccgatgttc agctgggcgt aggccatgcc ctccgggtag
     5641 ccgatcttat ccgactcccc gagggcctcc cgggcctgga ccagggcggc gtcgaagttc
     5701 tcgaacatac cgttccaggc cgactcgttg aggccgtcga tcc
//
LOCUS       DAOTEI010000202         5740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10036_length_5740_cov_4.308355, whole genome shotgun sequence.
ACCESSION   DAOTEI010000202 DAOTEI010000000
VERSION     DAOTEI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10036_length_5740_cov_4.308355"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="P5313_14860"
     CDS             <1..927
                     /locus_tag="P5313_14860"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRY81693.1"
                     /translation="PPPQWDASVREEKTPEGNVLLVAARTGELYFEGNRLAIVQGKQV
                     AGDVGPATGNVRFPGAVQVSGSVLGGYTVFAEGDVTISGSVEAALISSDGSIRIGEGI
                     KGRRKATLRARKTLEASFAEQALLLAVEGVSLRGACLLSNVKTNGRLVLGGDKGILIG
                     GVVRARRGVEVQSLGSENGIKTEVSFGQDYLLADQAEAEEREIERLKALILQTDKRMK
                     DLERSGFALDEVRQDKTKLLKLIEKRSLRVFDFRERFEEHHESAVVVRGVVHPGVVLE
                     SHNRFLEIQSRKERVAFTFDVQLGRIVEKPLK"
     gene            960..1928
                     /gene="galE"
                     /locus_tag="P5313_14865"
     CDS             960..1928
                     /gene="galE"
                     /locus_tag="P5313_14865"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRY81694.1"
                     /translation="MRILVIGGAGYIGSHVARAFLDRGAEVTVFDNLSTGVRENLFPE
                     ARFEEGDILDYGRLEAVMKGGYDALVHLAAFKAAGESMEKPEKYSVNNISGTLNILNA
                     ACAAGVRRIVFSSSAAVYGEPEYVPLDEGHPTNPENYYGFTKLEIERFLGWYDKLKGI
                     RFAALRYFNAAGYDEAGRVRGLERNPANLIPVIMEVAVGRRAELKVFGTDYPTPDGTG
                     VRDYVHVSDLAEAHVAGLEYIGKHDRSLTVNLGSEEGLSVLEILEAARKITGKPIPAA
                     MTARRPGDPAKLVASSARARELLGWTARRSDAATLVRTTWEAYTRD"
     gene            2427..3290
                     /locus_tag="P5313_14870"
     CDS             2427..3290
                     /locus_tag="P5313_14870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRY81695.1"
                     /translation="MDEKRRLGYFEGTLSAVLNTLLFGFKIWVGAAAGSVAMIADAWH
                     TMSDTLTSLVVILGFWISGKPGDRKHPFGHGRAELVAAVVIGTLLGVVGVNFLRDSAV
                     QLRERRGAEFDVAALIVFGVSVILKEAIARFSIRAGRRVKSQSLVADGWHHRSDALAS
                     ALILAGALLQRRFWWIDGVLGIGVSLLILYAAYGIIREAADTLLGECPDEDTVRGIRE
                     ILARLTPEATDIHHIHIHRYGDHVEVTLHIRMPPEMSVREAHGIATSLETELRQTLGW
                     EATVHLEPRQG"
     gene            3385..3687
                     /locus_tag="P5313_14875"
     CDS             3385..3687
                     /locus_tag="P5313_14875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRY81696.1"
                     /translation="MLAIAVTYKAKPGKREEALRAARTCSAETRREAGNADYTFYAGI
                     DDPQTFFLFEHWESREALDAHLKSSHLAAFRAALKDLLEAPPAIRIYEVSSFKNGL"
     gene            complement(3818..4813)
                     /locus_tag="P5313_14880"
     CDS             complement(3818..4813)
                     /locus_tag="P5313_14880"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRY81697.1"
                     /translation="MALIVQKFGGTSVADEKAREHLAAKAEAARARGDKVVLVVSAMG
                     RRGAPYATDTLLDLLSPFGNGADAANRDLMASCGEIVSACLVAAFLSSRGTPAVPMTA
                     FTAGIRAEGPYGDATPVAADAARIRAALDAGKVPVVTGFQGVNPEGQITTLGRGGSDT
                     TAVALAAFLGADYADIYTDVPGVAVADPRIVPDAPFLDYLDYQSMYRLAACGARVLHD
                     RSARIAQERGVRVRVLSTFDYKPGTLIGPERPGTPEKAFLGIAVDKKNPESSTVTILF
                     RTGRGGEGIGKATQAAAPWSRDRVPTEDPDVASFLCPAASAPDLAQALFAAFRTN"
     gene            complement(4868..5392)
                     /locus_tag="P5313_14885"
     CDS             complement(4868..5392)
                     /locus_tag="P5313_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY81698.1"
                     /translation="MPMSPDAPDASDRTLKTLGAPSLAELRAAGVVPSREELAAHPCV
                     CVECIEEIPCNPCETSCPQGALTVGQPITNLPVLDRQKCTACGLCIPACPGLAVVIKS
                     LEGDRAKIRFPWEYLPVPAKGDRVEMVDRLGNPVCGGTVVSVINPARNNRTAVVTAEF
                     PAEYVDDVISMRRL"
     gene            complement(5383..>5740)
                     /locus_tag="P5313_14890"
     CDS             complement(5383..>5740)
                     /locus_tag="P5313_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HRY81699.1"
                     /translation="VGLTPSIELASLAGCRTFFVPELGGHMPLHDEFMRSSVPAVYVV
                     GDIAGVEEASTAMEEGRLAGIHAAFSMGYLDSGEMERRTADTRRRLRALRQGVFGAKR
                     FTAKERILAEKEKECP"
BASE COUNT          943 a         1927 c         1995 g          875 t
ORIGIN      
        1 cccccgcccc agtgggacgc ctcggtccgg gaggagaaaa ccccggaggg caacgtcctt
       61 ctggtggccg cccgcaccgg ggagctctac ttcgagggaa accgccttgc catcgtccag
      121 ggcaagcagg tggccgggga cgtgggtccc gccaccggaa acgtccgctt ccccggggcc
      181 gtccaggtct cgggctccgt cctgggaggc tacaccgtct tcgcggaggg ggatgtcacc
      241 atctccggct ccgtggaggc cgccctgatc tcctcggacg ggtccatccg catcggggaa
      301 ggcatcaagg gccgccgcaa ggcgaccctc cgggcccgga agaccctgga ggcctccttc
      361 gcggagcagg ccctgctcct ggccgtggag ggagtatccc tccggggcgc gtgcctcctg
      421 tccaacgtga aaaccaacgg ccgcctggtc ctgggcgggg acaagggcat cctgatcggg
      481 ggggtcgtgc gggcccggag gggggtggag gtccagagcc tcggctccga gaacgggatc
      541 aagaccgagg tctccttcgg ccaggactac ctcctggcgg accaggcgga ggcggaggag
      601 cgggagatcg agcgtctcaa ggccctgatc ctccagacgg acaagcgaat gaaggacctg
      661 gagcggagcg gcttcgccct ggacgaggtc cggcaggaca agaccaagct cctcaagctc
      721 atcgagaagc ggagcctccg ggtcttcgac ttccgggagc gcttcgagga gcaccacgag
      781 tccgcggtgg tggtccgggg ggtggtgcat cccggggtgg tcctggaaag ccacaaccgc
      841 ttcctggaga tccagagccg caaggagagg gtggccttca ccttcgacgt ccagctgggg
      901 cgcatcgtgg agaagcccct gaagtgagaa tcccgggttc cggggaaggg gagaggatga
      961 tgaggatact ggtgatcgga ggggccgggt acatagggag ccacgtggcc cgggccttcc
     1021 tggaccgggg cgcggaggtc acggtcttcg acaacctctc cacgggggtc cgggagaatc
     1081 tctttcccga ggcccggttc gaggaggggg acatcctgga ctacggccgc ctcgaggccg
     1141 tgatgaaggg gggctacgac gccctggtcc acctggccgc cttcaaggcc gccggggagt
     1201 ccatggagaa gcccgagaag tactcggtga ataacatctc cgggaccctg aacatcctga
     1261 acgccgcctg cgccgccggg gtgcgccgga tcgtcttctc ctcctccgcg gcggtctacg
     1321 gggagcccga gtacgtaccc ctggacgagg ggcacccgac gaatccggag aactactacg
     1381 gcttcacgaa gctggagatc gagcgcttcc tgggctggta cgacaagctc aagggcatcc
     1441 gcttcgccgc cctgcgctac ttcaacgccg ccgggtacga cgaggccggc cgggtccggg
     1501 gcctggagcg gaatcccgcc aacctcatcc ccgtgatcat ggaggtggcc gtcgggcgcc
     1561 gggccgagct caaggtcttc ggcaccgact acccgacccc ggacggcacg ggggtccggg
     1621 actacgtcca cgtctccgac ctggcggagg cccacgtcgc gggcctcgag tacatcggga
     1681 agcacgaccg cagcctgacc gtcaacctgg gcagcgagga gggcctctcg gtcctggaga
     1741 tcctcgaggc cgcccggaag atcaccggga agcccatccc cgcggccatg accgcccggc
     1801 gtcccgggga tcccgccaag ctggtggcct cctccgcccg ggcccgggag ctcctgggct
     1861 ggaccgcccg ccgctccgac gccgccaccc tcgtccggac tacctgggag gcatacacta
     1921 gagactgaaa tcgtttcgga atactcgatg aagcccgcag ggcttcatcg ccgcgagggc
     1981 ggctcggccg ccctcgcgcg gactaggtta gagaaactcg acgaagccct gcgggcttcg
     2041 tcgccgtcga tccggcaaag ccggattgac gcgacgtggg aggcatacac tagagactga
     2101 aaccgtttcg gaatactcga tgaagcccgt agggcttcat cgccccgagg gcggctcggc
     2161 cgccctcgcg cggactaggg ttcctgggaa tcgacgaagc ccgaaggact gcggcgccgt
     2221 cgatccggcc aagccggatc gacgcgacac cccggcactt gacagggccc gggccgcgga
     2281 ttaccttcca gatcaggatc aggcggcctt cccccaccgg ggcgggcgga gccgggctac
     2341 acgcagcgga aaccacccgc gggaccacca ttcggccccg cgggtttttt atcgtcccgg
     2401 ggcctctcga aaggcggaac cgaacgatgg acgaaaaacg caggctgggg tacttcgaag
     2461 ggacgctgtc ggcggtcctc aataccctgc tcttcgggtt caagatctgg gtcggggcgg
     2521 ccgcgggttc cgtggccatg atcgccgacg cctggcacac catgtcggac accctgacct
     2581 ccctggtggt gatcctggga ttctggatct ccgggaagcc cggggaccga aaacacccct
     2641 tcggtcacgg ccgggcggag ctcgtggcgg ccgtcgtcat cgggacgctc ctgggcgtgg
     2701 tgggggtgaa tttcctgcgg gattccgccg ttcaactgag ggaacgccgg ggagccgagt
     2761 tcgacgtggc ggccctcatc gtgttcgggg tctcggtgat cctgaaggaa gccatcgccc
     2821 ggttctcgat ccgggccggg cgccgggtga aatcccagtc cctggtggcg gacggctggc
     2881 accaccggag cgacgccctg gcctcggcgc tcatcctggc cggggccctg ctccagcgga
     2941 gattctggtg gatcgacggg gtcctgggga tcggggtgtc cctgctgatc ctgtacgctg
     3001 cctacggcat catccgggaa gccgcggaca ccctcctggg tgagtgcccc gacgaggaca
     3061 ccgtccgggg catccgggag atcctggccc ggctcacccc cgaggccacc gacatccacc
     3121 acatccacat ccaccgctac ggcgaccacg tggaggtcac cctccatatc cgcatgcccc
     3181 cggagatgtc cgtacgcgaa gcccacggga tcgccacgag cctggaaacc gaactccggc
     3241 aaaccctggg gtgggaggcc acggtgcacc tggagccgcg gcaggggtga ggggcgaggc
     3301 tcggattccg gagcggggcg agcttctcaa tccgattccg gggacgtatg atgcttccat
     3361 tcgcacccat agggaggaca ccgtatgctc gccatcgccg taacgtacaa ggccaagccc
     3421 gggaagcgcg aggaggcgct gagggcggcg cggacctgct ccgccgagac ccgccgcgag
     3481 gccgggaacg cggactacac cttctacgcg gggatcgacg atccccagac cttcttcctc
     3541 ttcgagcact gggagagccg ggaggccctg gatgcccacc tgaagtcttc ccacctggcg
     3601 gccttccggg cggccctgaa ggacctgctg gaagctccgc ccgccatccg gatctacgag
     3661 gtgtcgtcct tcaagaacgg actctagcag gttgttgaaa tactcgaggc atttcaacaa
     3721 cctgctatgc aattgctcga gaaccatacg gttctctgcg caattgcgac atcaaggtag
     3781 tgttgaaaaa ggctctatag cctttttcaa caccctgcta gttcgtccgg aacgcggcga
     3841 agagggcctg ggccaggtcc ggggcggagg cggcggggca gaggaaggac gcgacgtccg
     3901 ggtcctccgt cgggacccgg tcccgggacc agggagccgc ggcctgtgtg gccttgccga
     3961 tgccctcccc gccccgaccg gtccggaaga ggatcgtgac cgtcgaggac tcggggttct
     4021 tcttgtccac cgcgatcccc aggaaggcct tctcgggggt cccgggacgc tcgggaccga
     4081 tcagggtccc gggcttgtag tcgaaggtgg acaggacccg gacccggacc ccgcgctcct
     4141 gggcgatccg ggccgagcgg tcgtggagga cccgggcccc gcaggccgcc aagcggtaca
     4201 tggactgata gtccaggtag tccaggaagg gggcgtccgg cacgatccgg ggatccgcga
     4261 cggccacccc cgggacgtcc gtgtagatgt ccgcgtagtc cgcccccagg aaggcggcaa
     4321 gggctacggc ggtggtgtcg ctcccccccc ggcccagggt cgtgatctgc ccctccggat
     4381 tgacgccctg gaagcccgtc accacgggga ccttcccggc gtccagggcg gcccggatcc
     4441 gggccgcgtc cgccgcgacg ggggtcgcgt ccccgtaggg gccctccgcc cggatccccg
     4501 cggtgaaggc ggtcatcggg accgcggggg tgccccggga ggagaggaag gccgccacca
     4561 ggcaggcgga gacgatctcc ccgcaggatg ccatcaggtc ccggttcgcc gcgtccgccc
     4621 cgttcccgaa gggcgaaagg aggtccagga gggtgtccgt ggcgtaggga gcgcccctcc
     4681 gtcccatcgc ggagacgacc aggacgacct tgtccccccg ggcgcgggcg gcctcggcct
     4741 tggcggccag gtgttcccgg gccttctcgt cggccacgga ggtccctccg aatttctgca
     4801 cgatcagggc catagattcc tccagaagtt tggttcggac atccgggacg gcgagccgct
     4861 cggcttttta aagcctgcgc atcgagatca cgtcgtccac gtactcagcg gggaattccg
     4921 cggtcaccac ggcggtgcgg ttgttccggg ccgggttgat caccgagacc acggtgcccc
     4981 cgcagacggg attccccaga cggtcgacca tctcgacgcg gtcgcccttg gccgggacgg
     5041 ggaggtattc ccaggggaac cggatcttgg ctcgatcccc ctccaggctc ttgatcacca
     5101 ccgcgaggcc cggacaggcg ggaatgcaca ggccgcaggc cgtacatttc tgacggtcca
     5161 gtacgggcag gttcgtgatg ggctggccca cggtcagggc gccttgggga caggaagtct
     5221 cgcagggatt gcaggggatc tcctcgatgc attccacgca gacgcaggga tgggcggcca
     5281 gttcctcccg ggagggcacg acccccgctg ccctcagttc cgccagagag ggagctccca
     5341 gggtcttcag ggtcctgtcg gaggcatccg gggcgtcggg gctcatgggc attccttctc
     5401 cttttccgcc aggatgcgtt ctttggccgt gaagcgcttt gctccgaaga ctccctgccg
     5461 gagggcccgg agccggcgcc gggtgtcggc ggtccgcctc tccatctccc cggaatccag
     5521 gtagcccatg gaaaaggcgg cgtggatccc cgcaaggcgg ccttcctcca tggcggtgct
     5581 ggcctcctcg acccccgcga tgtccccgac cacgtagacc gccgggacgc tggagcgcat
     5641 gaactcgtcg tgcaggggca tgtgccctcc gagctcgggg acgaagaaag tccggcaccc
     5701 cgccagggac gccaactcga tcgagggggt cagccccacg
//
LOCUS       DAOTEI010000203         5733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10051_length_5733_cov_4.105847, whole genome shotgun sequence.
ACCESSION   DAOTEI010000203 DAOTEI010000000
VERSION     DAOTEI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10051_length_5733_cov_4.105847"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="P5313_14895"
     CDS             <1..463
                     /locus_tag="P5313_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY81700.1"
                     /translation="TWVFGAVSTGYFLAALLGSLASPLVLERTGISPYLLLFLLRLTT
                     AGFIVLLALQGSAAGFAVFYLAMFCWNGMVNPPEGTVLNLRIPADRRASLLSVASLLV
                     QLGGLAGALAFGALVGPLKIPGVWFAAAAVFAASAPLYLAAHGAGKGTERT"
     gene            complement(573..1067)
                     /locus_tag="P5313_14900"
     CDS             complement(573..1067)
                     /locus_tag="P5313_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRY81701.1"
                     /translation="MSKALIAYVTKTGTTREIALVIEKTLKAAGVEARTLPMSEAGDL
                     SGYDRVILGAPINGMRLVPEAAAFVEAHGAELRTRPVAVFAVSYMHGAARGNWNRAIE
                     KSVRSAAEAAGARTSAIFPGRVGSPLPGFARLLFGVPRDLSADRVDLEAVRAWAAELP
                     KALG"
     gene            complement(1079..1522)
                     /locus_tag="P5313_14905"
     CDS             complement(1079..1522)
                     /locus_tag="P5313_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRY81702.1"
                     /translation="MKVFIECERGSRTKRTYGLDLELKRQSETLLPYPYPYGFVLGTR
                     ADDGDGVDCYVLTEEPLEHGRIVEVEPAGLLPMTEDEEEDGKILAVPPGRALDEFPEA
                     GRILRDFILGIFRKFPEVRVAVGDIRPREAALEFLAARAVPDWKE"
     gene            complement(1551..1886)
                     /locus_tag="P5313_14910"
     CDS             complement(1551..1886)
                     /locus_tag="P5313_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRY81703.1"
                     /translation="MDKLTILWTNADVGTFDKMVAMYARNSKTKGWWDEVTVVIWGSP
                     AKLAAESEVVQLKIRELLQVGVKVSACKACADQFGVTGKLTEMGVEVVYWGVPLTEIL
                     KGREPLLTI"
     gene            2050..2499
                     /gene="ndk"
                     /locus_tag="P5313_14915"
     CDS             2050..2499
                     /gene="ndk"
                     /locus_tag="P5313_14915"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRY81704.1"
                     /translation="MPVERTFTMLKPGVLARRVAGEIITRIERKGFIIRAVKTLRIDR
                     ALAEAHYAEHRGKDFFERLVAYITSGPVLAMVLEGDGAIAMLRKLCGSTKAEEAAPGT
                     IRGDYGMHTNLNIIHASDSPESAAREIGLFFRPDEILEWEDGSHDWI"
     gene            2486..4270
                     /locus_tag="P5313_14920"
     CDS             2486..4270
                     /locus_tag="P5313_14920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRY81705.1"
                     /translation="MTGSDALPRWNLDPIYAGFEAPEYRMDKEELGRLSEALLDHLRV
                     APPGAGFPAWLREALRLETEAGALEETLGAYAYTRYSTATTDPKALAELNNLEEMRLP
                     LKRAGVLFRNALAARKDEVQALVSADPGIGEYAFHLREEILWASRQMSPELEDLAEDL
                     NRAGGDAWGRLQEQVSSTAAMVWDEATGERKTTVQLRALSSDPDRAVREKAWDLELKA
                     WEQMKLPLAAALNGVKGFAVTLNKRRGWADPLEKSIEQCRITRRTLDALISAMEESLP
                     LWRRYLKAKARYLGVPACAFYDIFAPVGAPPAPWTFERVRAFIVEKFGAFSPDMGTFA
                     ERAFREGWIDAEPREGKVGGAYCISFPNRKASRVLCNFDGSFGEVSTIAHELGHAYHH
                     EVIKDVPHPLAQYPMTLAETASIFAETVVFEEAVKEVPQDQKAALLENNLQNGCQILV
                     DILSRFYFEKSVFARRPAAELGAEELCDLMREAQEATYGEGLDKDRRHPYMWAVKGHY
                     YSPDLSFYNFPYAFGLLFGLGLYEKYRRQGPSFAAAYRDILVRTGRMSAVDLTRQAGF
                     DIEGKEFWLGGIEVFKRQVEEFERLYRG"
     gene            complement(4338..5081)
                     /locus_tag="P5313_14925"
     CDS             complement(4338..5081)
                     /locus_tag="P5313_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRY81706.1"
                     /translation="MVSKSQLKFYASLKDRRARAESGLFIAEGHKAVQEGLKSGCPCE
                     AAFVREGFFGENEETDRLLSNQTVEVLEERDFRKLANTESPQGVLAVFDRRGLLAPSP
                     TERDLVVLYDLADPRNVGTIIRSADWFGFRDILVGETCVDIFNEKVVRSTMGSIFHCR
                     FILAADLAADLRALKPEYRVLTADTGGVDYRNRRRGGKTAVVFSNETRGPSDAILALT
                     DERITIPGFGRAESLNVSCAAAIILASYR"
     gene            complement(5200..>5733)
                     /gene="rsgA"
                     /locus_tag="P5313_14930"
     CDS             complement(5200..>5733)
                     /gene="rsgA"
                     /locus_tag="P5313_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRY81707.1"
                     /translation="REGWGLEGLDPYLAPGKTAVLLGSSGTGKSTLLNALAGSELQRV
                     QDVRADDHRGRHTTTTRTLFRLPSGALVIDTPGLREIQLWADGQAVDSSFSDIEAFAE
                     SCRFRDCSHDGEPGCAVRAAVESGDLAAERLESWRKLRREVEFLERRGDPVAEAAARA
                     VWRTRARAIQAMEKARR"
BASE COUNT          880 a         1991 c         1933 g          929 t
ORIGIN      
        1 cacctgggtc ttcggggcgg tttccacggg gtatttcctg gcggccctcc tgggcagcct
       61 ggcgtccccc ctggtcctgg agcggaccgg gatctcgccc tacctcctcc tcttcctgct
      121 ccgcctgacg acggcgggct tcatcgttct cttggccctg caggggtcgg cggcggggtt
      181 cgcggtattt tatctggcca tgttctgctg gaacgggatg gtcaaccccc cggaaggcac
      241 ggtgctcaac cttcggatcc ccgcggatcg cagggcttcc ctcctgtccg tggcatccct
      301 gctggtccag ctcgggggac tcgccggggc cctggccttc ggagccctgg tggggccctt
      361 gaagataccc ggggtctggt tcgcggcggc cgccgtattc gccgcctcgg cccccctgta
      421 cctggccgca cacggtgccg ggaagggaac ggagaggact tagccgtacc cggggaggtc
      481 gaagacctcg aaggtccgga agttcgaggg gttcgccgtg agcatgcggt cgacccccgc
      541 ttccgcgtag gccgcggcca tgtgggtgtc gatcagccca gagccttggg tagctccgca
      601 gcccaggccc ggacggcctc cagatccacc cggtccgcgc tcaggtcccg ggggacgccg
      661 aagaggagcc gggcgaagcc cggcagggga gaacccaccc ggccggggaa gatcgccgag
      721 gtccgcgccc ccgccgcctc cgccgcggac cgaacgcttt tctctatggc ccggttccag
      781 ttcccccggg cggctccgtg catgtaggat accgcgaata cggccacggg acgggtacgg
      841 agttcggccc cgtgggcctc cacgaaggcc gccgcctcgg ggaccaggcg catgccgttg
      901 atgggcgctc ccaggatcac ccggtcatat ccggacaggt ccccggcttc ggacatcggc
      961 aaggtccggg cctcgacccc cgcggccttc agggttttct cgatcaccag ggcgatctcc
     1021 cgggtggtcc cggtctttgt gacgtacgcg atcaaggcct tgctcatgga agtctccttc
     1081 attctttcca atccgggacc gcccgggcgg ccaggaattc cagggccgct tcccggggtc
     1141 ggatgtcccc cacggctaca cggacctccg ggaatttccg gaatatcccc aggatgaagt
     1201 cccggaggat ccgcccggcc tccggaaact cgtcgagggc ccggcccggc ggcacggcca
     1261 ggattttccc gtcctcctct tcgtcctcgg tcatggggag gaggcccgcg ggctcgactt
     1321 ccacgatccg gccatgttcc agcggttcct cggtgaggac gtaacagtcc accccgtccc
     1381 cgtcgtcggc ccgggtgccc aggacgaagc cgtaggggta ggggtagggc aggagggtct
     1441 cggactgccg cttcagctcc aggtcgaggc cgtaggtgcg cttggtccgg gaaccccgct
     1501 cgcactcgat gaagaccttc acggtttcac ccccttgtcc cccgatacat tcagatcgtc
     1561 aggagcggct cacggccctt caggatctcc gtgaggggga cgccccagta caccacctcc
     1621 acccccatct ccgtgagctt cccggtgacg ccgaactggt ccgcgcaggc cttgcaggcc
     1681 gacaccttga cgcccacctg caggagttcc cggatcttca attggaccac ttcggattcc
     1741 gcagcgagct tggcggggga tccccagatg accacggtca cctcgtccca ccagcccttg
     1801 gtcttggaat tccgcgcgta catggcgacc atcttgtcga aggtgccgac atccgcgttg
     1861 gtccagagaa tcgtcagctt gtccatgcac acctccgccg gggactacgg taaaccgcgg
     1921 tccggtgctt gtccaggagg ccccgggaaa aatcgccttg ctccctccct cccgggcgtg
     1981 tagtatggtc ccaggccccc cccggaacgc gccagcgcct tccgggatcg ggtccggaaa
     2041 ggagagtcca tgcccgtcga acgcaccttc accatgctga aacccggggt cctggcccgc
     2101 cgggtagccg gggagatcat aacccggatc gagaggaagg gattcatcat ccgcgcggtc
     2161 aagacactcc ggatcgaccg ggccctggcg gaggcgcact acgcggagca caggggcaag
     2221 gacttcttcg aacgcctcgt cgcctacatc acctccggac cggtactggc catggtcctg
     2281 gagggggacg gggcgatcgc gatgctccgg aagctctgcg gctccaccaa ggccgaggag
     2341 gccgcccccg ggacgatccg gggggactac gggatgcaca cgaacctgaa catcatccac
     2401 gcctcggact ccccggagag cgcggcccgg gagatcgggc tcttcttccg gcccgacgaa
     2461 atcctggaat gggaggacgg aagccatgac tggatctgat gccctgcccc gctggaacct
     2521 cgatccgatc tatgcgggct tcgaggcccc ggaataccgg atggacaagg aggagctcgg
     2581 tcgcctctcg gaagccctgc tggaccacct gagggtcgcg cccccggggg cgggtttccc
     2641 ggcctggttg cgggaggccc tgcgcctgga gaccgaggcg ggcgccctgg aggagaccct
     2701 gggagcctac gcctacaccc ggtactccac ggccaccacg gatcccaagg ccctggcgga
     2761 attgaacaac ctggaggaga tgcgtctgcc cctgaagcgg gccggggtcc tcttccgaaa
     2821 cgccctggcg gcccggaagg acgaggtgca ggccctggtc tccgcggacc ccgggatcgg
     2881 ggaatacgcc ttccacctcc gggaggagat cctctgggcc tcccggcaga tgtccccgga
     2941 gctggaggac ctggcggagg acctgaaccg ggcgggcggg gacgcctggg gccgccttca
     3001 ggagcaggtc tcctccaccg cggccatggt ctgggacgag gccacgggag agcgcaagac
     3061 cacggtccag ctccgggcct tgtcctcgga ccccgaccgg gcggtccggg agaaggcctg
     3121 ggacctggag ctcaaggcct gggagcagat gaagctgccc ctggccgcgg ccctgaacgg
     3181 ggtcaaggga ttcgccgtca ccctgaacaa gcgccggggc tgggccgatc ccctggagaa
     3241 gtccatcgag cagtgccgga tcacccgcag gaccctggac gccctgatct cggcgatgga
     3301 ggagagcctt cccctctggc ggcgctacct caaggcgaag gcccgatacc tgggggtgcc
     3361 cgcctgcgcc ttctacgaca tcttcgcccc cgtgggagct ccccccgccc cgtggacctt
     3421 cgagcgggtc cgggccttca tcgtggagaa gttcggggcc ttttccccgg acatggggac
     3481 cttcgcggag cgggccttcc gggagggctg gatcgacgcg gagccccggg aaggcaaggt
     3541 cgggggcgcc tactgtatct ccttccccaa ccggaaggcc tcacgggtcc tgtgcaactt
     3601 cgacgggagc ttcggggaag tctccacgat cgctcacgag ctgggccacg cctaccacca
     3661 cgaggtgatc aaggacgtcc cccaccccct ggcccagtac cccatgaccc tggcggagac
     3721 ggccagcatc ttcgcggaga ccgtggtctt cgaggaggcg gtcaaggagg tgccgcagga
     3781 ccagaaggcg gcgctcctgg agaacaacct ccagaacggc tgccagatcc tggtggacat
     3841 cctctccagg ttctacttcg aaaagagcgt cttcgcccgg cgtcccgccg cggagctggg
     3901 tgccgaggag ctttgcgacc tcatgcggga agcccaggag gccacctacg gggagggcct
     3961 ggacaaggac cgccgccacc cctacatgtg ggcggtcaag ggccattact attccccgga
     4021 cctctcgttc tacaacttcc cctacgcctt cggcctcctg ttcggcctgg ggctgtacga
     4081 gaagtaccgc cgccaggggc cgtccttcgc ggcggcctac cgggatatcc tggtacggac
     4141 cggacggatg agcgccgtgg acctgacccg tcaggcgggc ttcgacatcg agggcaagga
     4201 attctggctg gggggcatcg aggtcttcaa gcggcaggtg gaagagttcg aacggctgta
     4261 taggggatag gtgaatgggg gcgggaccgg acgggcggtt tgagcggccg ccgtgagccg
     4321 gcccgcccgg cggcctctca gcggtacgag gccaggatga tcgcggctgc gcaggacacg
     4381 ttgagggact cggcccggcc gaagcccggg atggtgatcc tctcgtccgt gagggccagg
     4441 atggcgtccg aggggccccg ggtctcgttg gagaacacga ccgccgtctt tccgccgcgc
     4501 ctccggtttc ggtagtcgac cccgccggtg tccgcggtca gcacccggta ctcgggcttg
     4561 agggctcgca ggtccgcggc gaggtcggcg gccaggatga agcggcagtg gaagatcgac
     4621 cccatcgtgg aacgcacgac cttctcgttg aagatgtcca cgcaggtctc acccaccagg
     4681 atgtcgcgga agccgaacca gtccgcggac cggatgatcg tacccacgtt gcgcgggtcg
     4741 gccaggtcgt agagcacgac caggtcccgc tccgtcgggg acggggccag gaggccccgc
     4801 cggtcgaaga cggccaggac cccctggggg ctctcggtgt tggccagctt gcggaagtcc
     4861 cgctcctcga gcacctccac cgtctggttg gaaaggagcc ggtccgtctc ctcgttctcc
     4921 ccgaagaatc cctcccgcac gaaggcggcc tcgcaggggc agcccgactt gagcccctcc
     4981 tgcaccgcct tgtgcccctc cgcgatgaac aacccgctct cggcccgggc cctgcggtcc
     5041 ttcagggaag cgtagaattt cagttggctc ttgctgacca tggccgtcct cggacccgag
     5101 tctagcatac ttgccggggt ttgatccggc cgccccccgt gcaggtcaca gggggacagc
     5161 cgcattcctg ttcaccgttc accgtctacc gtctaccgct cagcgcctcg ccttctccat
     5221 cgcttggatc gcccgggccc gggtcctcca gaccgcccgg gccgcggctt cggccacggg
     5281 gtcgccccgg cgttccagga actcgacctc ccgccggagc ttccgccagc tttccaggcg
     5341 ctcggcggcc aggtccccgg attccaccgc ggcccggacc gcgcagccgg gctccccgtc
     5401 gtgggagcag tcccggaagc ggcaggattc cgcgaaggct tcgatatccg agaaggagga
     5461 gtccaccgcc tggccgtccg cccagagttg gatttcccgg agccccgggg tgtcgatgac
     5521 cagggctccc gagggcaggc ggaagagggt ccgggtcgtg gtggtgtggc ggccccggtg
     5581 gtcgtcggcg cgcacgtcct ggaccctctg gagctccgag ccggccaggg cgttgagcag
     5641 ggtgctcttg cccgttccgg aggagcccag gaggaccgcg gtcttccccg gtgccaggta
     5701 gggatcgagg ccctcgaggc cccagccctc ccg
//
LOCUS       DAOTEI010000204         5720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10083_length_5720_cov_4.910680, whole genome shotgun sequence.
ACCESSION   DAOTEI010000204 DAOTEI010000000
VERSION     DAOTEI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10083_length_5720_cov_4.910680"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /gene="mrdA"
                     /locus_tag="P5313_14935"
     CDS             complement(<1..1439)
                     /gene="mrdA"
                     /locus_tag="P5313_14935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRY81708.1"
                     /translation="MSDLIPEAKPVDPRRILVFRLLAVGILAVYTGRLFYLQILRGGE
                     FRSQAASISRQSSILPSQRGEIYDRGGNIPLVLNVDSFALDIVPAELPAEMRDTVFLK
                     LAKLTGRPVEELRAKIPSSYYHLYQPVEVVGSVPYPVIVSVAERLDELPGVTWHAKPK
                     RNYVETGSLSHLIGYVGDITREELKLLFNQGYQSGDVIGKTGIEKQYDSLLRGRDGRE
                     YRVVDVRGRKIAGAEPSVDPPVMGRNIVLTIDRRIQELAEKALGQRMGSVVVLKPGTG
                     EILAMVSYPWYNPNLFLEADGGRAYARLLNDPNKPLLNRAIQSAYPPASTFKAVVTTG
                     ILEENAFPPEKTVLCQGEVSYGDRVFRCWIRKPGHGAVDLKGAMAQSCDIYFWTVARD
                     HLGIERLVSYSKEFGFGTASGIDLPGEISGFNPSPQWKERRYHEKWLGGDTMNMSIGQ
                     GYLLTTPLQMANMIAMIVNDGIIYKPHILS"
     gene            complement(1436..1942)
                     /gene="mreD"
                     /locus_tag="P5313_14940"
     CDS             complement(1436..1942)
                     /gene="mreD"
                     /locus_tag="P5313_14940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRY81709.1"
                     /translation="MFKTILVAVATSVGCSLLQSTWLHAVAVYAVIPDLALLVIVYVA
                     FQNVRTEGIAAGFLSGLLQDFISSAPLGFNAFVRTVVSFGFNLISGSFYIDRILMPML
                     FGGAATLLKALATALLAIAFPAFIHGYDFLDRVLWIETAYNAVLAPFLFLILGLLKSL
                     FVTARNRG"
     gene            complement(1944..2801)
                     /gene="mreC"
                     /locus_tag="P5313_14945"
     CDS             complement(1944..2801)
                     /gene="mreC"
                     /locus_tag="P5313_14945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRY81710.1"
                     /translation="MSDAAERQLARRRNLVFILALGASLLILLISTNSLAGVPQRVGL
                     SILSFFQKGFDAVGNFASGTLTSVSELRDLRARYRELVGRMEEYQNLERSLTDLKREN
                     ERLRSQLGYLDEARYRAASARIIAKDPGNLYSTFVIDKGMRDGLRKNLPVVAYQDGSE
                     GLVGRVLEVGKSSSIISPIFDSSAHISARLDRTRYEGLVSGGGSPDVPLTMRYIKKRA
                     KDEIQYGDLVVTSGFQSLYPRDVIIGRVTRILSADYQTSLDIDVEPVLDFARLEYVFV
                     VFPEAGTED"
     gene            complement(2801..3838)
                     /locus_tag="P5313_14950"
     CDS             complement(2801..3838)
                     /locus_tag="P5313_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRY81711.1"
                     /translation="MARRNLFDSLSLDIGIDLGTCNTLIYVRGKGIVINEPSVVAVER
                     GTKRVVAVGSEAKRMLWKTPGDIIAIRPLRDGVIADPDTTEKMIKYFIQKVIPRRWFV
                     KPRMVIGVPSCITEVERRAVEDAAYKAGAREVKVIHESLAAAIGSDIPIHEPAGHMIC
                     DIGGGTSEISVISLKGMVVTSAIRVGGDEFDEAIIKHIRNAHNLIIGEQTAERLKIEI
                     GNASPDKIIEKVEIKGTDAITGLPRRLEIDSVEVREALQEPINQIVEEIKRTLGQTPP
                     ELAADIVERGIVMAGGGSLLKGLPKLIAKETGVPVILAERPLECVAIGAGKFFETMNS
                     QDGSLYGSLNI"
     gene            complement(3853..4758)
                     /locus_tag="P5313_14955"
     CDS             complement(3853..4758)
                     /locus_tag="P5313_14955"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81712.1"
                     /translation="MIVLACLAAGALGIAFLVPALVRSARARNPRIPSEESILAAWNA
                     KDYDSVLALAAEGLAQRPLDPFRLVLSGFASFYRGVGETDGEIRTHYMDSAVFSLRKA
                     LTSPSVPFRSQVSYILGKAYYHKGPYYMDEAARHLEAAFQDSAGRGEAAPSDVLEYAA
                     VAYSALGEHVKSVEAFERALARGKTDLLLLAASRAYGLAGQADKAEITVLEALSLTED
                     SLVREKSRFLLGELYLARKDYGMAEEQYNLILEMNPESADAHYYLGLIWQEKGDPVRA
                     RAAWRKAVSLDPMHTAARQKLAERI"
     gene            complement(4904..5313)
                     /gene="rnpB"
                     /locus_tag="P5313_14960"
     ncRNA           complement(4904..5313)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5313_14960"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5325..>5720)
                     /locus_tag="P5313_14965"
     CDS             complement(5325..>5720)
                     /locus_tag="P5313_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRY81713.1"
                     /translation="QKQARVSELRASEKTATEGIDTEITFKFERIIRSKQGLGIVPVR
                     GYVCSGCSMILPAQFVNEVRQGNRIIFCPYCSRVLHYQEAEESEGDLIADIESGSLAD
                     LDDFSDEAEDDSYDEDSEDSEKEMGESDE"
BASE COUNT          991 a         1798 c         1876 g         1055 t
ORIGIN      
        1 gagaggatgt ggggcttgta gatgatcccg tcgttgacga tcatggcgat catgttggcc
       61 atctgcaggg gggtcgtcag gagatacccc tgtccgatgg acatgttcat cgtgtccccg
      121 cccagccatt tttcgtggta ccgccgctct ttccactggg ggctcggatt gaaaccggag
      181 atctcgccgg gaaggtctat gcccgaggcg gtcccgaacc cgaattcctt ggagtacgag
      241 acaagcctct cgattcccag atggtcccgg gcgacggtcc agaaataaat atcgcaggac
      301 tgggccatgg cgcccttgag atccaccgcc ccgtggcccg gcttgcggat ccagcaccgg
      361 aatacccggt cgccgtagga aacctctccc tggcagagga ccgtcttttc cggggggaac
      421 gcgttctcct ccaggattcc ggtggtgacc acggccttga acgtggaggc gggaggatac
      481 gccgattgga tcgcccggtt cagcaagggc ttgttcgggt cgttgagaag ccgcgcgtag
      541 gcccggccgc cgtcggcttc caggaagagg ttgggattgt accaggggta ggaaaccatg
      601 gccaggatct ccccggtgcc gggcttgagg acgaccacgg aacccatcct ctgtcccagg
      661 gccttctccg cgagttcctg tatcctgcga tcgatggtca ggacgatgtt ccggcccatg
      721 accggagggt ccacggacgg ttccgccccg gcgatcttgc gtccccgaac gtccacgacg
      781 cggtattcac gcccgtccct gccccggagg agcgagtcgt actgcttctc tatcccggtc
      841 tttccgatca cgtccccgga ttggtatccc tggttgaaca ggagcttgag ctcctcccgg
      901 gtgatgtccc cgacgtaccc tatgaggtgg gacagggatc ccgtctccac gtaattcctc
      961 ttgggcttcg cgtgccaggt caccccgggg agttcgtcca ggcgttcggc cacggacacg
     1021 atcaccgggt acggcacgga accgaccacc tccacgggct ggtacagatg atagtaggag
     1081 gacgggatct tggccctaag ctcctccacg ggccggccgg tgagcttggc gagcttcagg
     1141 aagaccgtat cccgcatctc cgcgggcagt tccgcgggca cgatgtccag ggcgaaggaa
     1201 tcgacgttga gcacgagagg aatgttcccg ccgcgatcgt agatctcccc gcgctgggac
     1261 ggaaggatcg aggactggcg ggatatgctg gcggcctggg aacggaattc ccctccccgg
     1321 aggatctgca ggtagaacag gcgacccgtg tagacggcca gtatcccgac cgccaggagg
     1381 cggaagacca aaatccgcct cggatcgacg ggcttggcct cgggtatcag gtcgctcatc
     1441 cgcggttccg cgccgtgacg aagagggact tcagcagccc caggataagg aacaggaacg
     1501 gagccaggac cgcgttgtac gccgtctcga tccagaggac cctgtccagg aaatcgtagc
     1561 cgtggatgaa ggccggaaac gcgatggcca ggagggccgt ggccagggcc ttgagcaatg
     1621 tggcggcccc tccgaagagc atgggcatca ggatccgatc aatgtagaaa ctgccggaga
     1681 tcaggttgaa gccgaaggag accacggtcc tcacgaaggc gttgaatccc aggggggcgg
     1741 atgagatgaa atcctgcagg agccccgaaa ggaatccggc cgcgatcccc tcggtgcgga
     1801 cgttctggaa ggcgacgtag acgatgacca ggagggccag gtcggggatc acggcgtaga
     1861 cggcgacggc atgaagccag gtggactgca gcagggaaca gcctacggaa gtcgctacgg
     1921 cgaccaggat ggtcttgaac acctcagtcc tccgtcccgg cttcggggaa gaccacgaag
     1981 acatattcaa gccgcgcgaa atccagcacg ggctccacgt cgatgtccag ggaggtttgg
     2041 taatcggcgg agaggatccg ggtcacgcgg ccgatgatga cgtcccgggg gtagagggac
     2101 tggaaacccg aggtgacgac gaggtcgccg tactggatct cgtccttggc cctcttcttg
     2161 atgtagcgca tcgtcaaggg gacgtcgggg ctgcccccgc cggacaccag tccttcatag
     2221 cgggtccggt ccaggcgggc ggagatatgc gcggaggagt cgaagatcgg ggagatgatg
     2281 ctggaggact tgccgacttc caggaccctg cccaccagtc cctcggagcc gtcctggtag
     2341 gccacgacgg gcaggttctt gcggaggccg tccctcatac ccttgtcgat cacgaaggtc
     2401 gagtagaggt tgccggggtc cttggcgatg atccgggcgg aggccgcgcg gtaccgggcc
     2461 tcgtccaggt agccgagttg ggacctgagg cgctcgttct cccgcttcag gtccgtcagg
     2521 ctgcgttcca ggttctggta ctcctccatc cgtcccacga gctcccgata ccgggcgcgg
     2581 agatcccgga gttcggagac gctggtcagg gttccggacg cgaaattgcc caccgcgtcg
     2641 aatcccttct ggaagaagga gagtatcgac agtccgaccc gctggggcac cccggccagg
     2701 gaattggtgg aaatgagcag gatgagcagg gacgccccga gggccaggat gaagaccagg
     2761 ttcctcctgc gcgccagctg gcgctccgcg gcgtcggaca ttatatgttc agcgacccgt
     2821 agagggaccc gtcctggctg ttcatggtct cgaagaactt gcccgccccg atggccacgc
     2881 actccagggg ccgttcggcc aggatcacgg gcacgcccgt ttccttggcg atcagcttgg
     2941 gaaggccctt gaggaggctg ccgcccccgg ccatgacgat gccgcgctcc acgatgtccg
     3001 cggccagctc cggaggggtc tggcccaggg tccgcttgat ctcctcgacg atctggttga
     3061 tgggctcctg gagggcttcc cggacctcca cggagtcgat ctccaggcgg cgcgggagac
     3121 cggttatggc gtcggtgccc ttgatctcca ccttctcgat gatcttgtcc ggggacgcgt
     3181 tcccgatctc gatcttgagc cgctccgcgg tctgctcccc gatgatcagg ttgtgggcgt
     3241 tgcggatgtg cttgatgatg gcctcgtcga attcgtcccc tcccacccgg atggcgctgg
     3301 tcacgaccat gcccttcagg gagatcacgg agatctccga ggttcctccg ccgatgtcgc
     3361 aaatcatgtg ccccgcgggc tcgtggatgg ggatgtcgga tccgatcgcg gcggccaggg
     3421 actcgtggat caccttgacc tcccgggcgc cggccttata ggcggcgtcc tcaacggccc
     3481 ggcgctccac ttcggtgatg caggacggca ctccgatcac catgcggggc ttcacgaacc
     3541 acctgcgggg gatgaccttc tggatgaagt acttgatcat cttctccgtg gtgtccggat
     3601 ccgcgatcac cccgtcccgg aggggtcgga tggcgatgat gtccccgggg gtcttccaga
     3661 gcatcctctt ggcctcggat ccgacggcca cgacacgctt ggtcccgcgc tcgacggcca
     3721 cgaccgaggg ctcgttgatt acgatgccct tgccccgcac gtagatcagg gtgttacagg
     3781 tcccgaggtc gatccctatg tccagggaga gggagtcgaa caggttgcgg cgagccatga
     3841 tgcctccagt cgttaaatcc gttccgcgag cttttgacgc gccgcggtat gcatgggatc
     3901 cagggatacg gccttgcgcc atgccgcgcg cgctcggacc gggtcgcctt tttcctgcca
     3961 gatcaatccc aggtaataat gagcgtcggc ggattccgga ttcatttcca ggataaggtt
     4021 gtactgctcc tcggccatcc cgtagtcctt acgggcgagg tagagttccc ccaggaggaa
     4081 tcgggacttt tcccggacca gggaatcctc cgtcagggag agggcttcca ggaccgtgat
     4141 ctcggccttg tcggcctggc cggcaaggcc gtaggcccgg gacgcggcca acaggagcag
     4201 gtcggtcttt ccccgggcga gagcccgctc gaaggcttcc acgctcttta cgtgctcccc
     4261 gagggcggaa tacgcgaccg ccgcgtattc caggacatcc gaaggcgctg cctcgccccg
     4321 gccggccgag tcctggaagg ccgcttccag atgccttgca gcctcgtcca tgtagtaagg
     4381 ccccttgtgg tagtacgcct tgccgaggat gtagctcacc tgggagcgga aggggacgga
     4441 aggggatgtc agggctttcc ggagggaaaa gactgcggaa tccatatagt gggtccgtat
     4501 ttcgccgtcc gtctccccga ccccgcggta gaacgaggcg aaaccgctca ggaccaggcg
     4561 gaagggatcc agggggcgtt gggccaggcc ttccgcggcc aacgccagga cgctgtcata
     4621 atccttggcg ttccaggcgg ccaggatgct ctcctccgag gggatccggg gattccgtgc
     4681 tcgggcggaa cgcacgagag cgggcaccag gaacgcgatg ccgagggccc cggcggccag
     4741 acaggcaagg acgatcatcc gcgtgagtcg ccttctccgg cgcgaggagc tgcgttcgaa
     4801 ggtgtccgat tttcccggga ttgcgtacat agggcctgca aatttttacc atgcacatac
     4861 ctcccgagtc aatcgacggg gaaccgcgat cgtccgcggg aaaaaaaggg gcaggtctgt
     4921 aagccgggtt ctgtgcgcct tgcggcgcca ccgccatctc tctcggcctc cggtctcccg
     4981 ggacctccag cggtctaccc gcggatcgcc gcgccctgac ggcgcggccg ggccggcggc
     5041 ccatccgctg ctcgaccttg ctcgcagcga ggcttgccat gccgtctccg tcgccggaga
     5101 cgcggtgggc tcttacccca ccctttcacc cttgccatgg acccttgcgg gcccattcgg
     5161 cggtctgctc tctgttgcgc tgtctgtcga cgcggcacgg tccgaagacc gccccgcgtc
     5221 gcccggccgt tagccggcgc tgtgccgtag cgagcccgga ctttcctccg ccccggtctt
     5281 gaagaccgga acggcggcgg ttcgacctgc ccccaaaaca accgtcattc gtcggattcg
     5341 cccatttcct tctcggaatc ttccgaatcc tcgtcgtagg aatcatcctc ggcctcgtcg
     5401 gagaaatcgt ccaggtccgc gaggcttccg gattcgatat ccgcgatcag gtcgccctcc
     5461 gactcctccg cctcctggta gtggagcacc cgggaacagt aggggcagaa gatgatccgg
     5521 ttgccctgcc ggacttcgtt gacgaactgc gcgggcagga tcatggaaca tccggaacat
     5581 acgtagccgc gcacggggac gatgcccaag ccctgcttgg aacggatgat ccgctcgaac
     5641 ttgaaggtga tctccgtgtc gatcccctcc gtggcggtct tctcggaggc ccggagctcg
     5701 gagacccggg cctgtttctg
//
LOCUS       DAOTEI010000205         5676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10182_length_5676_cov_4.740082, whole genome shotgun sequence.
ACCESSION   DAOTEI010000205 DAOTEI010000000
VERSION     DAOTEI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10182_length_5676_cov_4.740082"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            600..1043
                     /locus_tag="P5313_14970"
     CDS             600..1043
                     /locus_tag="P5313_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81714.1"
                     /translation="MEPLRDLTWMRYLLTGPPAERVERPGYAAVFVAIPRTKERHLER
                     YRFRAFVFEPSARSPVLAVNLESDILGEYILTLEDARGRSVLDRFDREPSYEEYRNRV
                     LEEAGRRMPPGGPAARPRGGAGTRRTRGGPSPEIKTKPPRESHEK"
     gene            1033..2238
                     /locus_tag="P5313_14975"
     CDS             1033..2238
                     /locus_tag="P5313_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81715.1"
                     /translation="MRSDRFPARRIPSRRFLILACAASLFLAAPERAPAEELYSDSGF
                     FLELPEGFTLNDGDGVSRFSFTDPSGVLLFQIFLYEPARYSGARAMQEDIGKKLGARA
                     DSEAFTYQRRGAVMADLSFPLSGKPHRGWGLFVDGAAGNAAGKPEKDIALLAYVPEER
                     YEAYLPFILSCLDGFSVDEEARLYPGPVSQFNLPWDPAARKPADLRFGDRTLRVPYDP
                     REGAAAQDTVEREFQVFGVYARSSPDLAHAAWARFYRMVYRETFHRLDFLSLVLQREL
                     SGEPDERSRTARLLAWTQGFRYERDPSGSDVVNPLTAAFEGRGDCDSRAVLLAILARH
                     DNRDALLMLSVKHSHALAAFDLPGPGARFPHKGKGWLVGETTDDVALGLIDAGMADPA
                     DWIAVEFPR"
     gene            2300..3193
                     /locus_tag="P5313_14980"
     CDS             2300..3193
                     /locus_tag="P5313_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRY81716.1"
                     /translation="MESISAKYRNVFIEAMKHTRIPETVTDDNVFQPANPEILPFIDR
                     IVGDNLLPGSGIRHFENLLELHREAREGKACLLLLEHYSNADLPAFHYLLRHHGPEGE
                     QLAQAVVAVAGIKLNEDNPLIRTIVEAYSRLVIYPSRSMEIIRSHLTDPKELFLEMMK
                     SRSINHAAMKELDVLKRSGRILLVFPAGTRFRPWDPGTKKGVREIDSYLKSFDRMVLV
                     SINGNILRINPEDPQMHVDLVCPDKVVYDASPVLDCREFRERVKHAAHFNDDKKQAVV
                     DAVMKRLDEMHEAVERDRLKA"
     gene            complement(3215..3913)
                     /locus_tag="P5313_14985"
     CDS             complement(3215..3913)
                     /locus_tag="P5313_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81717.1"
                     /translation="MPLRSAGHARTALLLACLLSARGIACQDLVLRAEFWAESEPVEV
                     LPAEITDTAPKVTVPEEVVRSLLEEGRRVFSGMIWGFEFRYVPYDKARGVAEELEIVP
                     LGEIPWGDPRLGTSQTRLTGTQLRAYLEYRPDPSAAARKAAWDDATFQSAQGRGSAPL
                     AGGREARKAALDQAAKEALRELLRPVEKNKPREIRGVFAYDASPRIVITGGSYTAVVR
                     IRVKVAEVRRYSVY"
     gene            complement(4157..5494)
                     /locus_tag="P5313_14990"
     CDS             complement(4157..5494)
                     /locus_tag="P5313_14990"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRY81718.1"
                     /translation="MVILTLNCGSSSVKYQVYDWAAQSILAAGIVERVTQSGSVISHK
                     VPGREEFTQNHECPNHTVAIELILNTITGAETGVIKGMSDIKAVGHRMVHGGSKFARS
                     CLIDDEFLATFKSLNDLAPLHNPANLLGVEAARSVLPKVPQCAIMDTAWHQTMPESSY
                     LYAVPYEWYEKNMVRRYGFHGTSFLYNAKRAAVLLGKDPFKTNLIIAHIGNGASINAV
                     KDGCSFDTSMGLTPLEGLVMGTRSGDIDPGIIFHMIRQTGMSAADIEKKLNKESGVLG
                     VTGKWADRRDVEMGAEKGDPRALAALHLESYRIKKYIGAYYAALGRVDALVFTAGVGE
                     MGPIIRALATEGLAEMGIKIDPEKNKLAKCRNAELEITGAGSKVRVFVIPTDEELVMT
                     EDAYALISGKYDVHTKYTYSFQRKDYVNKARAEGLARDLEKKPYLKDLIAKVP"
BASE COUNT          943 a         1892 c         1860 g          981 t
ORIGIN      
        1 gctccaggac ggtcaggtct tcccgggacg cccaccggac gtccacccag gtgccggggc
       61 ccgcggactc cgcggggacc aggactccgt cctgttgttt ccagattcgg atcatgattg
      121 taccctcccg cccccgggag ggtcgcgcgc ggttcgacgg cgttcaggaa ggctgtcgaa
      181 gggcgggtaa cccggctctc gggggccagc gttggaacga gataggtgac ggacaactac
      241 cgtcgggatc cattcgcttc ccccttcttg gcgccgaagc gccggtatgg cggccccacg
      301 gggcgccggg tccggccggg gaccgcctta aggcggtatc ctgcctgatt ccaagcccag
      361 tatagcgccg gggccggatc gttgcaatga cggagatccc gatttgccct cggccttccc
      421 tgccgatata cttggtagag agctaggagc ccgtcggact tgtggacttt ttccgcctat
      481 aggcgtaata agtcccgatt atcgggctcc tcaggagtat gcaaggtaga aaaatcgcat
      541 gtatgcgatt tttccttgat tgtcgtggga aagcccgaca aactcctagg agggaacggg
      601 tggaacccct gcgggatctt acctggatgc gatacctctt gaccggcccc cccgccgaac
      661 gggtggagcg gccgggctac gcggcggtct tcgtggcgat cccgcgcacc aaggagaggc
      721 acctggaacg ataccggttc cgcgccttcg tgttcgagcc ttccgcccgg tccccggtgc
      781 tggcggtgaa cctggaatcc gacatcctgg gggagtatat cctgaccctg gaggatgccc
      841 gggggcggag cgtcctggac cggttcgacc gggaaccgtc ctacgaggag taccggaacc
      901 gggtcctcga ggaggcgggg cggaggatgc ctcccggggg tccggccgca cggccccgcg
      961 gcggagccgg aacacggcgc acccggggcg ggccctcccc ggaaatcaag accaagcccc
     1021 cgagggagtc ccatgagaag tgaccgcttt cccgcccgcc ggatcccgtc ccgtcgattc
     1081 ctcatcctgg cctgcgccgc gtccctcttc ctggccgccc cggagcgcgc cccggcggag
     1141 gagctctact ccgattccgg cttcttcctc gagctccccg agggcttcac cctgaacgac
     1201 ggggacgggg tctcccggtt ttccttcacc gatccctccg gggtcctctt gttccagatc
     1261 ttcctgtacg agcccgcccg ctactccggg gcccgggcga tgcaggagga catagggaag
     1321 aaactgggcg cccgggcgga ttccgaggcc ttcacctacc agcgccgagg cgccgttatg
     1381 gcggacctga gtttcccgct gtccggcaag ccccacaggg gttgggggct attcgtggac
     1441 ggggccgcgg ggaacgccgc gggcaagccc gagaaggaca tcgccctcct ggcctacgtg
     1501 cccgaggagc ggtacgaggc ctacctgccc ttcatcctct cctgcctgga cggtttttcc
     1561 gtcgacgagg aggcccggct ctacccgggc ccggtgagcc agttcaacct gccctgggat
     1621 ccggcggcgc ggaagcccgc ggacctccgc ttcggggatc ggaccctccg ggtcccctac
     1681 gatccccggg agggggccgc ggcccaggac acggtggagc gggagttcca ggtcttcggc
     1741 gtgtacgccc ggtcctcccc cgacctggcg cacgcggcct gggcccggtt ctaccggatg
     1801 gtctaccggg agaccttcca ccgtctggat ttcctttccc tggtgctcca gcgggaactt
     1861 tcgggggagc cggacgaacg gtcccggacc gcccgcctgc tggcctggac ccagggcttc
     1921 cggtacgagc gggacccctc gggaagcgac gtggtgaatc cccttacggc cgccttcgag
     1981 ggccggggag actgcgattc ccgggccgtc ctcctggcca tcctggcccg ccacgacaac
     2041 cgggacgccc tgctcatgct ctcggtcaag cattcccatg ccctggccgc cttcgacctg
     2101 ccgggccccg gcgcccggtt cccgcacaag ggcaaggggt ggctagtcgg ggagaccacg
     2161 gacgacgtgg ccctgggcct catcgacgcc gggatggcgg accctgcgga ttggatcgcg
     2221 gtcgagtttc cacgttgacc gcccttcccg cgggggacta tactggaatc gccccccaat
     2281 cctgttccaa ggccaggcga tggaaagcat aagcgcgaag tacaggaacg tcttcatcga
     2341 ggcgatgaag catacccgga ttccggaaac cgtcacggac gacaacgtct tccagccggc
     2401 caacccggag atccttccct tcatcgaccg gatcgtgggg gacaacctcc tgcccggcag
     2461 cgggatccgg cacttcgaga accttttgga acttcaccgc gaggcccggg agggcaaggc
     2521 ctgcctcctc cttttggagc actacagcaa cgccgacctc cccgccttcc actatctcct
     2581 gcggcaccac ggccccgagg gggaacagct ggcccaggcc gtcgtggccg tggcggggat
     2641 caagctgaac gaggacaacc ccctgatccg gaccatcgtg gaggcctatt cacggctggt
     2701 gatctacccc agccgatcca tggagatcat ccggagccac ctgaccgatc ccaaggaact
     2761 cttcctggag atgatgaaga gccggagcat caaccacgcg gccatgaagg aactggacgt
     2821 actgaaacga tccggccgga tcctcctggt cttccccgcg ggcacccggt tccgcccctg
     2881 ggatccgggc accaagaagg gcgtccggga gatcgattcc tacctgaaat ccttcgaccg
     2941 gatggtcctg gtctccatca acggcaacat cctgcggatc aatcccgagg atccccagat
     3001 gcacgtggac ctggtctgcc cggacaaagt ggtctacgac gcgagccccg tccttgactg
     3061 ccgggagttc cgggagcgcg tcaagcacgc cgcccatttc aacgacgaca agaagcaggc
     3121 cgtggtggac gcggtgatga aacggctgga cgagatgcac gaggcggtgg agcgggaccg
     3181 gctgaaggct tgaaccgtcg cccttccggc gaattcagta gaccgaatac cttctaacct
     3241 cggcgacctt cacccggatc ctcacgacgg cggtgtacga acccccggtg atcacgatcc
     3301 gcggcgaggc gtcgtaggcg aatacccccc ggatctcccg gggcttgttc ttttccaccg
     3361 gccggaggag ttcccggagg gcttccttgg ccgcctggtc cagggcggcc ttccgggcct
     3421 cccgccctcc cgccaggggg gccgaacccc ggccctgggc ggactgaaag gtagcgtcat
     3481 cccaggccgc cttacgggcc gccgcggacg ggtccggacg gtattccagg taggcccgga
     3541 gctgggttcc ggtcagccgg gtctgggaag tcccgaggcg gggatcgccc caggggatct
     3601 cccccagggg aacgatctcc agctcctcgg ccaccccccg ggccttgtcg taggggacgt
     3661 accggaactc gaagccccag atcatcccgg aaaagacccg tcggccctcc tccaggaggg
     3721 accggacgac ctcctccggg accgtgacct tcggggccgt gtcggtgatc tcggccggca
     3781 ggacttccac gggctcggat tccgcccaga actccgcccg caggacgaga tcctggcacg
     3841 cgataccgcg cgccgagagg aggcaggcga gaaggagggc ggtccgggca tgaccggcgg
     3901 agcggagggg catacttcaa tgttcggaag aaacggattc ccgatggaat cgaatccgga
     3961 gccctgaaaa tcaacgcgac ccggccatgc cgggtcgcgc gacaggggct tcgcctgaga
     4021 ccgtcaggga accttggcga tcagatcctt caggtacggc ttcttctcca aatcgcgcga
     4081 gccgggtcca cccgactcgc ggcgacgaag gccgctaggc ggccttcgtc gatcctcagg
     4141 gcctcgcccg caccgttcag ggcaccttgg cgatcagatc cttcaagtac ggcttcttct
     4201 ccaaatcgcg ggcgaggccc tcggcgcggg ccttgttcac gtaatccttg cgctggaagg
     4261 aataggtgta cttggtgtgg acgtcgtact tgcccgaaat cagggcgtag gcgtcctcgg
     4321 tcatcacgag ctcctcgtcc gtggggatca cgaaaacccg caccttggac ccggccccgg
     4381 tgatctcgag ctccgcgttc cggcacttgg ccagcttgtt cttctcggga tcgatcttga
     4441 tgcccatctc cgcgaggccc tcggtggcca gggcacggat gatcggcccc atctccccca
     4501 ccccggccgt gaagaccagg gcgtccacgc ggcccagggc ggcgtagtag gcgccgatgt
     4561 acttcttgat ccggtaggac tccaggtgca gggccgccaa ggcccgggga tcccccttct
     4621 cggcccccat ctccacgtcc cgacggtccg cccacttgcc ggtcacgccc aggacgccgc
     4681 tctccttgtt cagcttcttc tcgatgtccg cggcggacat cccggtctgc cggatcatgt
     4741 ggaagatgat ccccgggtcg atgtccccgg accgggtccc catgaccagg ccctccaggg
     4801 gggtgaggcc catggaggtg tcgaaggagc agccgtcctt gacggcgttg atggaggccc
     4861 cgttgccgat gtgggcgatg atcaggttgg tcttgaaggg atccttgccc aggaggaccg
     4921 cggcccgctt ggcgttgtac aggaagctcg tcccgtggaa gccgtagcgc cggaccatgt
     4981 tcttctcgta ccactcgtag ggcacggcgt agaggtagct cgactcgggc atggtctggt
     5041 gccaggcggt gtccatgatg gcgcactggg gcaccttggg gagcaccgac cgggcggcct
     5101 cgacgcccag gaggttggcg ggattgtgga ggggggccag gtcgttcagg gacttgaagg
     5161 tggccaggaa ctcgtcgtcg atcaggcagg accgggcgaa cttggacccc ccgtggacca
     5221 tgcggtgtcc cacggccttg atgtcggaca tgcccttgat caccccggtc tcggctcccg
     5281 tgatagtgtt caggatgagc tcgatggcga cggtgtggtt cgggcactcg tggttctggg
     5341 tgaactcctc ccggcccggg accttgtggc tgatgacgga cccgctctgg gtgacccgct
     5401 ccacgatacc ggcagccagg atggactggg cggcccagtc gtaaacctga tacttgacgg
     5461 aagaacttcc gcagttgagt gtgaggatga ccatggatgc tccttacgga tggatgcccc
     5521 agtataccag gaacccggga attggggaag aaaagttcca ataaatatgc gtatttttcc
     5581 cccgaatgcc tggttctacc atagatgcgg gtactccgga cccgcggagg cggagggctt
     5641 cgtcgggaaa gacgaggtca cgtggttcgg cggcac
//
LOCUS       DAOTEI010000206         5577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10430_length_5577_cov_5.617892, whole genome shotgun sequence.
ACCESSION   DAOTEI010000206 DAOTEI010000000
VERSION     DAOTEI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10430_length_5577_cov_5.617892"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="P5313_14995"
     CDS             complement(<1..889)
                     /locus_tag="P5313_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81719.1"
                     /translation="MYKRAGRVLFGSLALILASAGLLSAQAKLPGGVTSRLGKIEAYL
                     KSTEARIQNGSTNRNDLDMAIELMKEIKKSYPETASHPDVQAAEKRIAEVEAALAAAL
                     SGKAQAKAASAASAAASEQALVDWARRLAEYKPDGRPGSKGLFGVPTEDIDQLLAQKP
                     NYEKAKVVYAEFLASGLDKDAHPELRQAEYDIKVGILNYERSRDRIPEEAAAKVDEAL
                     AWMNARQAEGKAIALEPGVKKRVDILVEQSGLLFPGSSRTRALIAKKAELDKRLEEMD
                     KTILEKRVMSPDVYKGKDGE"
     gene            complement(1014..2498)
                     /locus_tag="P5313_15000"
     CDS             complement(1014..2498)
                     /locus_tag="P5313_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRY81720.1"
                     /translation="MREVPYLGRRYAAIAYSIGVILVVSGAIQAVPILAAVWYPEETA
                     RIPAFLVPAGIQTLLGLALGLAFRSRRTVELNVREGGVIIVVGWTIVILFSAWPFTAV
                     LGLPFSRSVFESVSGWTTTGLSVVDVLAAGPAILLWRSVMQLSGGAGLAVIMMSAVIG
                     PAGTGISSAEGRGDQLVPQVKRSARLVLTVYLSYAAAGIAGYRLAGMGWFDAINHSFA
                     AVSTGGFSTRVESVGHWDSAAIEAVTLPLMILGNLSFVTAWHLFRGRLRTVGRNGEIR
                     LLAVVIPASVTLAFLFTTRELYPRMDKAFRVAVFEVVTAITTTGFSTVGYGNWNSFGV
                     FLIVILMLIGGGTCSTAGGIKQSRVYLLWKALVRELRTMAAPRRAVLSVPYWEGERRV
                     FLDDGKVRQVAAFVVLYLVTYAAGTLVLCACGFGLTESLFEFASALGTVGLSVGLTGA
                     NMPDAALWAETLAMFLGRLELTIVFVSLAKIGTDARNAFARHSR"
     gene            complement(2495..3175)
                     /locus_tag="P5313_15005"
     CDS             complement(2495..3175)
                     /locus_tag="P5313_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRY81721.1"
                     /translation="MKALIIGGGTSIYFLCRNLASRGFRVAVIDRDPEVCGRLSRFLP
                     VEVVCADGSDAGVLEEAGARKAELVLAVTGNDADNLVACQLASMEFGVPRTAALANDP
                     ENAEAFQDLGVPSFSIAEVVGSLIEQRASLREITNVLPAGGGKVIVTELVLQENSPVA
                     GKLLKEVALPENSLVAILIRGEETIVPRGGNDLRAGDRLVLVTLPDNHGPAIRAVTGE
                     RSGTGGGV"
     gene            complement(3172..3612)
                     /locus_tag="P5313_15010"
     CDS             complement(3172..3612)
                     /locus_tag="P5313_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRY81722.1"
                     /translation="MRRNRYVVVAGCGRLGSGIAARLSASGDSVVIIDREESAFAGLP
                     PEFSGFRIEGDASRLSVLKAAKVDEADFLIAATGSDNVNLMAAQVAKRTFGVARVLAR
                     VYDPTKEKMYSGLGIVTVCPTSTAAEMFFRVMTEEPSGRGEASL"
     gene            complement(3633..4322)
                     /locus_tag="P5313_15015"
     CDS             complement(3633..4322)
                     /locus_tag="P5313_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY81723.1"
                     /translation="METIRMLVVDDEKNIRFTMSKSLEGLGAQIRTAVNGEEALGMLH
                     DEEYSLVFLDLKLPGMDGLEVLRRIREGYPRIRVVIISAHGSIGSAVDAMKLGAVDFI
                     QKPFSPDEIRGVAAGILAREDLDESSADDYPSLIELAKRRIADRDFTAARAAAERAIA
                     LDPARAEAYNLVGVVLEIKGEGLAAQKFYRASSDIDPTYRPAWANLERVTGLSGSGRI
                     DLDHGEQRRRR"
     gene            complement(4344..>5577)
                     /locus_tag="P5313_15020"
     CDS             complement(4344..>5577)
                     /locus_tag="P5313_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY81724.1"
                     /translation="ADRIARPLGRLMEASRRISGGDYTVEVPKDTGDELGRLAGEFNE
                     MARRLSAYRETDVDRLIAEKNKSEAILSSIEDGLVVFDPEGRVTALNPTARRLLALEF
                     AETATLRLEDVLPEDPARDYVRRTLESGTRPDVPEERRILRLPAGDGEAIHCLYSVFA
                     VRGRDRNLSGIVLLLRDVTRLKDLERLKEEFVMAASHELRTPLTSLGMSVELLRERAG
                     PGLPDGDRELLATAHEEVQRMKALVSDLLDLSRMEAGRIEMEIESVSVRDMFERVESV
                     FRNQAKMKDVRLRVLGPEGELRVRADPGKAAWVLTNLVSNALRYVEPGGHVDLFAQRI
                     GPLAYLSVRDDGTGIPVESQARIFEKFTRIREGGTGGSGLGLAISKEIVRAHGGAIWV
                     ESPSGGRGSTFTFTLPAE"
BASE COUNT          917 a         1953 c         1758 g          949 t
ORIGIN      
        1 cctccccgtc cttgcccttg tatacgtcgg gcgacatcac gcgcttttcc aggatggtct
       61 tgtccatctc ctccaggcgc ttgtccagct cggccttctt cgcgatgagg gcgcgggtcc
      121 ggctcgatcc ggggaagagc aggccggatt gctcgaccag gatgtcgacg cgtttcttga
      181 cgcccggctc cagggctatg gcctttcctt ccgcctgccg ggcgttcatc catgccaggg
      241 cctcgtccac cttggccgcc gcctcctccg gtatccggtc ccggctccgt tcgtagttga
      301 ggatgccgac cttgatgtcg tactcggcct gccggagctc cgggtgggcg tccttgtcca
      361 ggccggaggc caggaactcg gcatagacga ccttggcctt ctcgtagttc ggtttctggg
      421 ccaggagctg gtctatatcc tcggtgggaa cgccgaacag gcccttcgag ccgggccgcc
      481 cgtcgggctt gtattccgca agccgccttg cccaatcgac cagggcctgt tcgctcgcgg
      541 ccgcactcgc cgcggaggcg gccttggcct gggccttgcc ggacagggcc gccgccaggg
      601 cggcttccac ctcggcgatc cgcttctccg cggcctgtac gtcgggatgg gaagcggtct
      661 cgggatagga cttcttgatc tccttcatga gctcgatggc catgtccaga tcgttccggt
      721 tggtggagcc gttctggatg cgcgcctcgg tgctcttgag gtaggcctcg atcttcccga
      781 ggcggcttgt gacccctccg ggcagtttgg cctgcgcgga gagaagtccc gccgaggcga
      841 ggatgagggc gagcgacccg aacaggacgc ggcctgcacg tttatacatg gcgttcctcc
      901 tgagtttgta aaaaagtatc gaatgtgcct gccggaacgt caagaagccg cccggaatcc
      961 gcctggaatc caccttcccc gggagtgtgc aggggacgcg acagccgcct tcctcagcgg
     1021 gaatgccggg cgaaggcgtt ccgggcgtcg gttccgatct tggcgaggct cacgaagacg
     1081 atggtcagtt cgagccggcc caggaacatg gccagggtct cggcccagag cgcggcatcg
     1141 ggcatgttcg cgcccgtgag gcccacggag agtccgaccg taccgagggc cgaggcgaac
     1201 tcaaacaagg attccgtcag accgaagccg caggcgcaga gaaccagggt tcccgcggcg
     1261 taggtaacaa ggtacaggac tacgaaggcg gccacctgcc ggacctttcc gtcgtccagg
     1321 aagacccgcc tctccccttc ccaatagggg acggacagca cggcgcgcct cggagccgcc
     1381 attgtccgca gttcccggac cagggccttc cagagcagat acacccggct ctgtttgatc
     1441 ccccccgcgg tggaacaggt ccctcccccg atgagcatca ggatcacgat gaggaacacc
     1501 ccgaaggaat tccagttccc gtaccccacc gtggaaaatc ccgtcgtcgt tatggccgtg
     1561 acgacctcga agaccgcgac acggaacgcc ttgtccatcc gcggatacag ctctcgagtc
     1621 gtgaaaagga acgcgagggt gacggaggcg gggatgacga ccgcgagcaa tcggatctcc
     1681 ccgttcctgc ccacggtccg gagcctgccg cggaagagat gccaggccgt gacgaagctc
     1741 aggtttccca ggatcatgag gggaagggtc accgcctcga tcgcggcgga gtcccagtgc
     1801 ccgacgctct cgacccgggt ggagaagcct ccggtggaca ccgccgcgaa ggaatggttg
     1861 atcgcgtcga accagcccat tccggccagg cggtacccgg cgatacccgc cgcggcatag
     1921 gagaggtaca cggtgaggac cagccgggcg gatcttttaa cctggggaac cagctgatcc
     1981 ccccggccct ccgcgctgga aattcccgtg ccggccggtc cgatgacggc ggacatcatg
     2041 atcaccgcca aacccgcacc gccggacaat tgcatgacgc ttcgccacag caggatcgcc
     2101 ggtcccgccg cgaggacgtc cacgaccgac aggcccgtgg tggtccaccc gctcaccgat
     2161 tcaaaaaccg accgggaaaa cggcagcccc agcacggccg tgaacggcca ggcggaaaaa
     2221 aggatgacga tcgtccaccc cacgacgatg atcacgcctc cctcccggac gttcagctcc
     2281 acggtccgcc tcgaccggaa cgccagcccc agggctaggc ccaggagcgt ctgaattccc
     2341 gccggaacca ggaaagccgg gatccgggcg gtctcctcag gataccagac ggccgcgagg
     2401 atcggcacgg cctgtatcgc gccggacacg accaggatga ctcctatcga gtatgcgatc
     2461 gcggcgtaac gccgccccag gtagggtact tccctcatac gccgcctccg gtcccggatc
     2521 tctcccccgt gacggcccgg atcgcaggtc cgtggttgtc cggcagggtc accagcacga
     2581 ggcggtcccc ggcccgcaag tcgtttcctc cccgggggac gatggtctcc tcgccccgga
     2641 tcaggatggc caccaagctg ttctccggca gggcgacctc cttgagaagt tttcccgcga
     2701 cgggggaatt ctcctggagc accagctcgg tcacgatgac cttgcctccg ccggcgggga
     2761 ggacgttggt gatctcccgg agggacgccc gctgctcgat gaggctcccc accacttcag
     2821 cgatcgagaa actcggcact cccaggtcct ggaacgcctc cgcgttttcg gggtcgttgg
     2881 ccagcgccgc cgtcctaggc acgccgaact ccatcgaggc gagctggcag gccaccaggt
     2941 tgtccgcgtc gttcccggtc accgcgagca ccagctccgc cttcctcgcg cccgcctcct
     3001 ccaggacccc cgcgtcgctc ccgtccgcac acaccacttc gacgggaagg aaccgggaga
     3061 ggcgtccgca cacctcggga tcgcggtcga tcacggcgac tcggaacccc cgggacgcga
     3121 gattgcggca cagaaaatat atcgaggtgc ctcccccgat aatcaacgcc ttcataagga
     3181 ggcttcccct cgtccggagg gctcctcggt catgacccgg aaaaacatct ccgccgcggt
     3241 ggaggtggga cagaccgtga cgatcccgag ccccgaatac attttttcct tggtcggatc
     3301 gtagacccgg gcaaggacgc gtgcgacccc gaaggtccgc ttggccacct gggccgccat
     3361 caggttgacg ttgtcgctgc cggtcgccgc gatcaggaaa tcggcctcgt ccaccttggc
     3421 ggccttcagg accgagaggc ggctcgcgtc cccctcgatc cgaaaaccgc tgaattccgg
     3481 cggcaatccg gcgaacgcgg attcttcccg atcgatgatc accacggaat ccccggaggc
     3541 gctcaaccgc gccgctatgc ccgagcctag tcggccgcag cccgcgacca cgacgtatcg
     3601 attgcgccgc atatgtcctc ctcgggcgcc ggctaccggc gtcgcctctg ctctccgtgg
     3661 tccaggtcga ttctcccgga tccggataac cccgtgaccc gttccaggtt cgcccatgcg
     3721 ggacggtagg tcggatcgat gtcggaggaa gcccggtaga atttctgggc cgccagaccc
     3781 tctcccttga tctccaggac cacccccacc aggttgtagg cctccgcccg cgccgggtcc
     3841 agggcgatcg ccctctccgc cgcggcccgg gcggccgtga aatcccggtc ggcgatgcgg
     3901 cgcttggcga gctcgatcag ggagggatag tcgtccgccg aggactcgtc caggtcctcc
     3961 cgggccagga tccccgcagc gacgccccgg atctcgtccg ggctgaaggg tttctggatg
     4021 aagtccacgg ccccgagctt catggcatcg accgcggacc cgatggatcc gtgggcgctg
     4081 atgatgacca cccggatccg gggatatccc tcccggatcc tccgcaggac ctccaggccg
     4141 tccatgcccg gcagcttcag gtccaggaac accagggagt attcctcgtc gtgaagcatc
     4201 cccagggctt cctcgccgtt cacggccgtc cgaatctggg cccccaggcc ctccagggac
     4261 ttagacatcg tgaagcggat gttcttttcg tcgtcgacga cgagcatccg gatagtttcc
     4321 atcgatcgct cctttccgtc ttctcactcc gcgggcaggg tgaacgtgaa cgtgcttccg
     4381 cggccgccgg agggagactc gacccagatg gcccctccgt gggcccggac gatttccttg
     4441 ctgatcgcga gccccagacc cgagccgccc gtcccgcctt cccggatcct cgtgaatttc
     4501 tcgaagatcc gggcctgcga ctcgaccggg atgccggtcc cgtcgtcccg caccgacagg
     4561 taggccaggg gaccgatcct ctgggcgaag aggtccacgt gcccgccggg ttcgacatac
     4621 cgcagggcgt tggagacgag gttggtgagc acccaggcgg ccttgccggg atcggcccgg
     4681 acccgaagct ccccttcggg ccccagtacg cgaagccgga cgtccttcat cttcgcctgg
     4741 ttccggaaca cggactccac gcgctcgaac atgtcccgga ccgagacgct ctcgatctcc
     4801 atctcgatcc ttccggcttc catccgggag aggtcgagca ggtcgctcac cagggctttc
     4861 atgcgctgga cctcctcgtg ggccgtcgcg aggagttccc ggtccccgtc gggaagaccg
     4921 ggtccggccc gctcgcggag cagctcaacg ctcatcccca ggctggtgag gggtgtgcgg
     4981 agttcgtggg aggcggccat cacgaattcc tccttgagcc gttcgaggtc cttgagccgg
     5041 gtcacgtccc ggagcaggag cacgatcccg gacaggttgc gatcccgtcc ccgtacggcg
     5101 aatacggagt agaggcagtg gatcgcctcc ccgtcccccg cgggcagacg gaggatccgc
     5161 cgctcctccg ggacgtcggg ccgggtcccg gattccaggg tcctccgaac gtagtcccgg
     5221 gccggatctt cggggaggac gtcctcgagc ctcagcgtgg cggtttccgc gaactccagg
     5281 gcgaggagcc tgcgggccgt cggattgagg gccgtcacgc ggccttccgg atcgaagacg
     5341 acaagcccgt cctcgatgct ggacaggatc gcctcgctct tgttcttctc cgcgatcaac
     5401 cggtctacgt ccgtctcccg ataggcgctg agccgccggg ccatctcgtt gaactctccc
     5461 gccaggcgtc ccagctcgtc cccggtatcc ttcggaacct cgacggtata gtcgccgccg
     5521 gagatccgac gggaggcctc catgagccgc ccgagggggc gcgcgatgcg gtccgcg
//
LOCUS       DAOTEI010000207         5492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10679_length_5492_cov_3.849182, whole genome shotgun sequence.
ACCESSION   DAOTEI010000207 DAOTEI010000000
VERSION     DAOTEI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10679_length_5492_cov_3.849182"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="P5313_15025"
     CDS             complement(<1..1051)
                     /locus_tag="P5313_15025"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRY81725.1"
                     /translation="MMEETLSYDDVLLLPGYSEILPREADVRTRLAADIRLNVPILSA
                     AMDTVTEDRLAIALALEGGAGVIHRNLPPAEQAEHVSTVKRYLNWIIDSPVTIESDRT
                     IRDVRSLMSRHGVTGLPVLDQEGRVVGIITARDLRFSRDDGTLVRDAMTPDPVVVPGD
                     TTPAQARQKFDQYKIEKLPVVDAAGRLTGLITVKDMEKREIHPRAAVDSGGRLVVGAA
                     VSPQDIEARMPLLAAARCDFVVLDTAHGDSRNVAEAVRAIKAKYPIPVVGGNVATREG
                     ARRLVEAGADAVKVGVGPGSICTTRIVAGIGVPQFTAVLWCAEEASKAGIPVIADGGI
                     KYSGDVVKAIAAGASS"
     gene            complement(1180..2601)
                     /locus_tag="P5313_15030"
     CDS             complement(1180..2601)
                     /locus_tag="P5313_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRY81726.1"
                     /translation="MQSRDIFDNLSPLDHRYWEANRELFDALSERLSDRASVRACVLA
                     EIALLKAHLAARGLGTEERFRDLDALAGSVDPEEVAREEEKTRHNIRALVNVLQSKVP
                     ADLAPLVHLGATSVDILDTARAVQLRGAVLEVLLPLLARVLAELARLAEEEADTPQVG
                     RTHGRHAVPVTFGFALAEYAARLGKCLPRLEDLARDLRGKLAGATGSYNALSMIYPDP
                     EEVERTYLGFLGLSPSEHSTQLVEPEHVLRLLLELNTAFGILANLADDLRHLQREEID
                     EVRESFGGAQVGSSTMPQKRNPWNSEHVKSLWKAFCPRVLTFFLDQISEHQRDLTNSA
                     SERFSVEFLAGLGAAANRMLKVLKSLSVDRERMAVNLRSSGGAVLAEPAYILLAESGR
                     ADAHEEVRRLTLQAESEGLSFSEVLARRPEIRDILYGRLRDLGHPDPEGFFAAPERYR
                     GLAAEKARALGRKYRELARRWAD"
     gene            complement(2660..3142)
                     /gene="ybaK"
                     /locus_tag="P5313_15035"
     CDS             complement(2660..3142)
                     /gene="ybaK"
                     /locus_tag="P5313_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRY81727.1"
                     /translation="MTKTNAVRILESSGLPFRLAEYPVDEDDLSAPAVADKVGLPAEQ
                     VFKTLAARGDRTGPFLACVPGNAELSLKKAAVATGNKGVEMLPLRELEPLTGYVRGGC
                     SPVGTRKRLPVFLDETAQLFPEVSVSAGTRGLQMILAPEDLLAAVRLGNLSAEWADLV
                     "
     gene            complement(3150..4736)
                     /locus_tag="P5313_15040"
     CDS             complement(3150..4736)
                     /locus_tag="P5313_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HRY81728.1"
                     /translation="MERGYIALVLNAHLPFVRQPEYPRFLEERWLFEALSETYLPLLR
                     VLRNLEAEKVPVRLTLVLSPTLTAMLSDEVLRDRYVAWLDRQIGLAEREKERTAGDPE
                     FARLALLHADLYTSARDDFTILYGGNVLSGFDYFYKKGRLEIMTSAATHAFLPLYRDV
                     PEAVAAQVETAVISHRRQFGKVASGFWLPQLGWYPGMEEVLKAYSVRYTLVATRGALL
                     GKPVPKYGSYSPVTCPNGLSVFIRDAQATNAVWSEQTGYPADPVYRDFYRDIGFDLPL
                     DYVSPWLEDGARGFTGFKYWAVTGKTERKKPYDPAAASARAAEHARVFLDGRADQIRK
                     ASAYMDRPPLVVCPYDAELLGHGWFEGPQWLEALFRLGKDREEFRFVSLGEYLKLHPE
                     NPVSVPEFSSWGSGGYAGVWLDGSNDWIYRHSYKMLDRMMELAERFPNETGLKERVLN
                     QAARETLLVMCSDWALLIRAGKAGAFARRQVEDAVSNFQRIYEMLCANTVGTEWITRL
                     EKRNNLFPSINYRMFRRKRA"
     gene            complement(4739..>5492)
                     /locus_tag="P5313_15045"
     CDS             complement(4739..>5492)
                     /locus_tag="P5313_15045"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HRY81729.1"
                     /translation="TIENPYGEDRVVNIEKLSALSDEALLTLADKLGLIVPAGLERSF
                     LVEALLEALAEDSEEREEALDSSERLEETKYGGTDADILETSLDSGWCSDNRYNETMI
                     RLLVRDTAWAFAFWDVNDEEKAQLRAGEPGPGFPGLFLRVMEEGGETPRNADHFDIPV
                     DESDLQWYINLPQPDSRYRIDLCCRAGGKTRVLARSNVVQTPRGALAEDSAAMEGGTV
                     ELLALSGFGELDIPPSEDRHPSRILSGSEVEE"
BASE COUNT          831 a         1886 c         1910 g          865 t
ORIGIN      
        1 ccgaggaagc ccccgcggct atggccttga ccacgtcccc ggaatacttg atgcccccgt
       61 ccgcgatcac ggggatcccg gccttggacg cctcctcggc gcaccagagc acggcggtga
      121 attggggaac cccgatgccc gccacgatcc gggtggtgca gatggaaccc ggccccaccc
      181 cgaccttcac cgcgtccgcc cccgcctcga ccaggcggcg ggcgccttcc cgggtggcca
      241 cgttcccgcc caccacgggg atcgggtact tggccttgat agcccggacc gcctccgcca
      301 cgttccggga gtccccgtgg gccgtgtcca ggaccacgaa gtcgcagcgg gccgcggcca
      361 ggaggggcat ccgggcctcg atgtcctggg ggctcaccgc ggcgcccacc accaggcgcc
      421 cgccggaatc caccgcggcc ctggggtgga tctcccgctt ctccatgtcc ttgacggtga
      481 taagcccggt gagtcgcccc gcggcgtcca ccacggggag cttctcgatc ttgtactggt
      541 cgaacttctg ccgggcctgg gcgggggtcg tatccccggg caccacgacg gggtccgggg
      601 tcatggcgtc ccggaccagg gtcccgtcgt cccggctgaa ccggaggtcc cgggcggtga
      661 tgatccccac cacccggccc tcctggtcca ggacgggaag acccgtcacc ccgtggcggg
      721 acatcaggga acgtacgtcc cggatggtcc ggtccgactc gatcgtcacc ggggagtcga
      781 tgatccagtt gaggtagcgc ttcacggtgg agacgtgctc ggcctgctcc gcgggcggga
      841 ggttccggtg gatgaccccc gcccctccct ccagggccag ggcgatggcc agacggtcct
      901 cggtgaccgt gtccatcgcg gcggagagga tcggcacgtt cagccggata tccgcggcca
      961 gccgggtccg gacgtccgcc tcccggggga gtatctcgga ataccccggg agaaggagca
     1021 cgtcgtcgta ggagagggtc tcctccatga tcagtcgcct ccgcgcgaga ggctcacggc
     1081 ctctccggtc gatagagact cgtccggccg ccctcggccg ccgtcgagct ttcagggccg
     1141 cccgcaaaaa cccgtcgccg ccgggcggcc gccgttcctt caatccgccc agcgccgggc
     1201 gagttcccgg tacttccttc cgagggcccg ggccttctcc gccgcgagac cccggtagcg
     1261 ctcaggggcc gcgaagaatc cctcggggtc ggggtgcccc aggtcccgga gccggccgta
     1321 caggatgtcc cggatctccg ggcggcgggc caggacttcg gagaaggaga ggccctccga
     1381 ctcggcctgg agggtcagcc tgcggacctc ctcgtgggcg tccgccctgc ccgactcggc
     1441 caggaggatg taggccggct cggccaaaac ggcgcccccg ctggaccgga ggttgaccgc
     1501 catgcgttcc cggtccacgg acagggactt gaggaccttg agcatccggt tggccgcggc
     1561 ccccaggccc gccaggaact ccacggaaaa gcgctcgctg gcggagttcg tcaggtcccg
     1621 ctggtgttcg gagatctggt ccaggaaaaa cgtgaggacc ctgggacaga aggccttcca
     1681 gaggctcttc acgtgctcgc tgttccaggg attccgcttc tggggcatgg tggaggaacc
     1741 cacctgggct ccgccgaagg actcccggac ttcgtcgatc tcctctcgct ggagatgccg
     1801 caggtcgtcc gccaggttgg ccaggatgcc gaaggccgtg ttcagttcca gcaggagcct
     1861 aaggacgtgc tcgggctcca cgagctgggt ggaatgctcg ctgggcgaaa ggcccaggaa
     1921 gccgagatag gtccgttcca cctcctcggg gtccgggtag atcatggaca gggcgttgta
     1981 ggaacccgtt gccccggcca gcttgccccg gaggtcccgg gccaggtcct ccaggcgggg
     2041 caggcacttt cccaggcggg cggcgtactc cgccagggcg aagccgaagg tgacgggcac
     2101 ggcgtggcgg ccgtgggtgc gacccacctg gggggtatcc gcctcctcct cggccaggcg
     2161 ggccagctcg gcgaggaccc gggccaggag gggaagcagg acctcgagga cggcgccccg
     2221 gagctgtacc gcccgggccg tgtccaggat gtcgacgctg gtggctccca ggtggaccag
     2281 gggggccagg tccgcgggca ccttggactg gaggacgttc accaaagcac ggatgttgtg
     2341 ccgggtcttc tcctcctccc gggccacctc ctcggggtcc acggagcccg ccagggcgtc
     2401 cagatcccgg aacctctcct ccgtccccag gccccgggcc gccaggtggg ccttgagcag
     2461 ggcgatctcc gccaggacgc aggcccgtac ggaggcccgg tccgaaagcc gctccgacag
     2521 ggcgtcgaag agctcccggt tggcctccca gtaccgatgg tccagggggg agaggttgtc
     2581 gaagatgtca cgggactgca taccgcagta tgcccggccg gggtccgcac gtcaactacg
     2641 gcggcgcggg ccgccgcgct cagaccaggt ccgcccactc cgcggagagg tttcccagac
     2701 gtaccgcggc caggagatcc tccggggcca ggatcatctg gagtccccgg gtcccggcgc
     2761 tcacggagac ttccggaaac agctgggcgg tctcgtccag gaacaccgga agccgcttcc
     2821 gcgtcccgac cggggagcat ccgccccgga cgtatcccgt caggggctcc aattcccgga
     2881 gcggaagcat ctcgacgccc ttgttgcccg tggcgacggc ggccttcttg agggacagct
     2941 ccgcgtttcc gggcacgcag gccaggaagg gtccggtccg gtccccccgg gccgccaggg
     3001 tcttgaagac ctgttccgcg ggcaggccta ccttgtcggc cacggcgggg gccgacaggt
     3061 cgtcctcgtc cacggggtac tccgcgagcc ggaagggcag ccccgaggat tccaggatgc
     3121 gcacggcgtt cgtcttggtc atggaggggc taggcccgtt ttctgcggaa catccggtaa
     3181 ttgatgctcg ggaagaggtt gttcctcttt tccagccggg tgatccactc ggtccccacg
     3241 gtgttggcgc agagcatctc gtagatccgc tggaagttgg aaaccgcgtc ctccacctgc
     3301 ctgcgggcga aggcgccggc cttgccggcc cggatcagga gggcccagtc ggagcacatg
     3361 accaggaggg tctcccgggc cgcctggttc aggacccgct ccttgaggcc cgtctcgttg
     3421 gggaatcgtt cggccagctc catcatccgg tccagcatct tgtagctgtg gcggtagatc
     3481 cagtcgttgg acccgtccag ccacaccccg gcgtaccccc cgctccccca ggaggagaac
     3541 tcggggacgg atacggggtt ttccgggtgc agcttgaggt actcccccag ggacacgaaa
     3601 cggaactcct cgcggtcctt ccctagccgg aacagggctt ccagccactg gggcccctcg
     3661 aaccatccgt ggcccaggag ctccgcgtcg taggggcaga ccaccagggg cggccggtcc
     3721 atgtaggccg aggccttccg gatctggtcc gcccgtccgt ccaggaagac ccgggcgtgc
     3781 tcggcggccc gggccgaggc ggcggcggga tcgtagggtt tcttccgctc ggtctttccg
     3841 gtgacggccc agtacttgaa tcccgtgaag ccccgggccc cgtcctccag ccagggggag
     3901 acgtagtcca ggggaaggtc gaagccgatg tcccggtaga agtcccggta gaccgggtcc
     3961 gcggggtaac cggtctgctc ggaccagacc gcgttcgtcg cctgggcgtc ccggatgaac
     4021 acggacagcc cgttcgggca ggtcacgggg gaataggagc cgtacttcgg gacgggtttt
     4081 cccaggaggg cgccccgggt ggccaccagg gtgtagcgca ccgagtaggc cttcaggacc
     4141 tcctccatcc cgggatacca gcccagctgg ggcagccaga acccggacgc gaccttcccg
     4201 aactgccggc ggtgggagat cacggcggtc tccacctggg cggccacggc ctcggggacg
     4261 tcccggtaca ggggcaggaa ggcgtgggtg gccgcggagg tcatgatctc cagccggccc
     4321 ttcttgtaga agtagtcgaa gcccgagagc acgttcccgc cgtacaggat cgtgaagtcg
     4381 tcccgggcgg aggtgtacag gtccgcgtgg agcagggcca ggcgggcgaa ctcggggtcc
     4441 ccggcggtcc tctccttctc ccgctcggcg agcccgatct gccggtccag ccaggccacg
     4501 taccggtccc ggagcacctc gtcggagagc atggcggtca gcgtgggcga caggaccagg
     4561 gtgagccgca cgggaacctt ttcggcttcc aggttccgca ggacccggag gaggggcagg
     4621 taggtctccg agagggcctc gaagagccag cgttcctcca ggaaccgggg atactcgggc
     4681 tggcggacga aggggagatg ggcgttcagc accagggcga tatagcctcg ctccatcctc
     4741 actcctcgac ttccgatccg gacaggatgc gcgacgggtg gcgatcctcc gagggcggga
     4801 tgtccagctc cccgaagccc gagagagcca ggagttccac ggtcccgccc tccatggcgg
     4861 cggaatcctc cgccagggcg ccccgggggg tctgcaccac gttggaccgg gccagcaccc
     4921 gggtcttccc tccggccctg cagcaaaggt cgatgcgata gcggctgtcc ggctggggca
     4981 ggttgatgta ccactggagg tcggattcat ccacggggat gtcgaagtgg tccgcgttcc
     5041 gcggggtctc tcccccctcc tccatcaccc ggagaaacag gcccgggaag cccgggccgg
     5101 gctccccggc ccggagctgg gccttctcct cgtcgttcac gtcccagaag gcgaaggccc
     5161 aggcggtatc ccgcaccagg agccggatca tggtctcgtt gtagcggttg tcggaacacc
     5221 atccggaatc cagggaggtt tccaggatgt cggcatcggt gccgccgtat ttcgtctctt
     5281 ccagtcgttc ggaggagtcc agggcctcct cccgctcctc ggagtcctcc gccagggcct
     5341 ccaggagggc ctcgaccagg aaggaacgct ccagacccgc gggcacgatc agtcccagct
     5401 tgtcggcgag ggtgaggagc gcttcgtcgg aaagggccga gagcttttcg atgttcacta
     5461 ccctatcttc tccgtaagga ttttcgattg tc
//
LOCUS       DAOTEI010000208         5488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10690_length_5488_cov_4.336094, whole genome shotgun sequence.
ACCESSION   DAOTEI010000208 DAOTEI010000000
VERSION     DAOTEI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10690_length_5488_cov_4.336094"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="P5313_15050"
     CDS             <1..215
                     /locus_tag="P5313_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRY81730.1"
                     /translation="WFASYGPYGAGITDDTVVVVVMVEATNPWEWWAPYASNIIFQGI
                     FANQTYDEVIQALGLSYMIKPTERVE"
     gene            212..1531
                     /gene="rodA"
                     /locus_tag="P5313_15055"
     CDS             212..1531
                     /gene="rodA"
                     /locus_tag="P5313_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRY81731.1"
                     /translation="MIANLKALFSDIDWYILAASLILMVVGILFIFSSGVTSTGVVVS
                     TEYLRQIAWVALGFVFLMLVVLVDYKRVKDSSFVIYAAVLLLLVYTRMFGRVVNGARS
                     WIGIGELGIQPSEFAKIATIFYLSKYLDQTRHSLPGFRRFLGSLAIVGIPMLLVLSQP
                     DFGTALVFIPILLSMSYLCGLERRYIVFLLSTVALTLVLVVLPLWERNIAARPTGLLF
                     VLYEGPYRFWILGACVITLGLAVWGFLVFRNRYYYWISYAFLILILSLVGSELGHRVL
                     KDYQIMRLIVFLDPSVDPRGSGWNILQSITAIGSGGFAGRGFLQGTQSHYRFLPQQST
                     DFIFSILSEEWGFLGGLVVFGLYGIILWRTLALLKTVKDPFAVGIVAGLSGMILFHFM
                     INVGMTMGIMPITGIPLFFLSYGGSSLWAVLIGIGLILGISARRYHS"
     gene            1576..>3695
                     /locus_tag="P5313_15060"
     CDS             1576..>3695
                     /locus_tag="P5313_15060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRY81732.1"
                     /translation="MEKPARYLGGEQGAVRKEGSLFTVALCFPDLYEIGMSNHALRIL
                     YAGLNALPGIRCERVFAPARDFEQALRETGTPLYTLESGIPLSEVDLLAVTIGYELSA
                     TNLLTVLDLGGIPLDASSRGNGNPIVIAGGPAVTNPLPISRFLDAVWIGEAEAGFFKL
                     AVDLANLGSGEAGRSARIARLREEPAVWMPGKPAVRAVDMEFPDREYSGTFPWPAIKP
                     VQDHGTVEIMRGCPNGCRFCHAGYFYRPQRVRSPERIFREVESLILESGYREITLSSL
                     SSGDYPCLVDLVRELNRRWAGARISFQLPSLKVESFDLSILEELTHTRKSGLTFAVET
                     PEEAWQRSINKSVTLDKILAILGEAAHHGYRLAKFYFMIGLPIPGVSGIAETDALVGF
                     LGRLLKETRLEFNVNIGTFIPKPHTPFQWCSQIGEDEAFSRIRRIRDSFRHERRMKIS
                     YHSPFVSLLEGILSRGDERVGDLILEAWRNGARLDAWEDLMEKDTWRRAMGGVPEASI
                     REALDGRTLDSPLPWDSISLRVGKGFLKQEWEKTAASTMTSECTDNCTNPCGVCRDDG
                     SAVIKNIQPIMAEERSTPIGRHPGESSVPVGSKYRLVLKYRKTGPAAFLPHHSVMDMF
                     HRAFQLARYPVAFSQGFNPAPRLEIEDPIPLGMASLEDYISVLLTAPVDEKDTVVALN
                     RYLPADLAIQRCAMYVIPEGVKIRS"
     assembly_gap    3696..3705
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3706..4042
                     /locus_tag="P5313_15065"
     CDS             <3706..4042
                     /locus_tag="P5313_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81733.1"
                     /translation="RSLSSMAWGSDYLAEGVPPNSGAALTESLVHPRLEGSCLLPSGD
                     VIGTLSFRLRVGGTREIGFGSIFEKSFGLPFAGSPIQVTRLRQWADIGTEPLDFFKAF
                     DSLYGSAIS"
     gene            complement(4118..4828)
                     /locus_tag="P5313_15070"
     CDS             complement(4118..4828)
                     /locus_tag="P5313_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81734.1"
                     /translation="MGKINHLIPSLQIIVLVPFFSLFILGCGIDSYVYLAPPRNVIQN
                     GTTLHFENDTGNDISIFRGYILLYRFFKSSEDAASSRTQIASAFSSSPTTIFLRIQNL
                     GYNELIVNGSPYINVTNMPERSDPFSIEVSFDTRNSTGVFIDFAPSSAVVSINPGFPA
                     LISRKPKNLSSEFFSFKDSDFVENDSDLKSGQYDLDTGLTYLQCYVLSYGFSLDGVES
                     IYSEPIWFNGSLLSFSMQ"
     gene            complement(4848..>5488)
                     /locus_tag="P5313_15075"
     CDS             complement(4848..>5488)
                     /locus_tag="P5313_15075"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY81735.1"
                     /translation="ELGGEDKELTVFFSDIRGFTTLSENMAPQELVNHLNVYFTAMTN
                     LLMDYGGTLDKYIGDAIMGFWGAPLPQPDHAVRGCKCALAMMNRLKELNESWPEPKRI
                     NVGIGVNSGIITVGNMGSPLRMNYTLTGDHVNLASRLEATNKEYGTQVIVSEFTYGLV
                     KDKFIVRELDNIRVKGKNKPVGIYELVDCLEPLEPPKAPEAAGPRKKGRAEG"
BASE COUNT         1072 a         1643 c         1536 g         1227 t
ORIGIN      
        1 cctggttcgc cagttacggc ccctacggcg cgggcatcac ggacgatacc gtggtggtgg
       61 tcgtcatggt ggaagccacg aatccctggg agtggtgggc cccctacgcg tccaacatca
      121 tcttccaggg gatcttcgcg aaccagacct acgacgaagt catccaggcc ctcggactct
      181 cgtacatgat caagccgacg gagcgtgtcg aatgatcgcc aacctcaagg ccctgttcag
      241 cgacatcgac tggtacatcc tggccgcgtc cctgatcctc atggtcgtgg gcatcctgtt
      301 catcttttct tccggcgtga cgagcaccgg ggtcgtggtg tccacggaat acctgcggca
      361 gatcgcttgg gtggccctgg gtttcgtgtt cctgatgctc gtggtcctgg tggactacaa
      421 gcgcgtgaag gactccagct tcgtcatcta cgcggccgtc ctcctgctgc tcgtctatac
      481 ccgcatgttc gggagggtcg tcaacggggc ccggtcctgg atcgggatcg gggaactggg
      541 aatccagcct tccgaattcg ccaagatcgc cacaatattc tacctgtcca agtacctgga
      601 ccagacccgg cattcccttc ccggcttccg gcgtttcctg ggctccctcg ccatcgtggg
      661 aatccccatg ctcctggtgc tgtcccagcc ggatttcggc acggccctcg tcttcatccc
      721 catcctcctt tccatgtcgt acctctgcgg ccttgagcgc cggtacatcg tgttcttgct
      781 ttccaccgtg gccctgaccc tcgtcctcgt ggtcctgccg ctttgggaga ggaacattgc
      841 cgcccgaccc acgggcctgc tcttcgtcct ctacgagggt ccctaccggt tctggatcct
      901 cggagcctgc gtgatcacac tggggcttgc cgtctgggga ttcctcgtct tccggaatcg
      961 gtattactac tggatatcct acgcgttcct gatcctgatc ctctccctgg taggttccga
     1021 actcgggcac cgggtcctca aggattacca gatcatgcgg ctcatcgtgt tcctggatcc
     1081 ctccgtggat ccccggggtt ccgggtggaa catcctgcaa tccatcaccg ccatcggttc
     1141 gggaggattc gcgggccggg gattcctcca gggaacccag agccattaca ggttcctgcc
     1201 ccagcagagc acggatttca tattctcgat cctctccgag gagtggggct tcctgggcgg
     1261 gcttgtcgtg ttcgggcttt acggaatcat actctggagg accctggccc ttctgaagac
     1321 cgtcaaggat ccgttcgcgg tcggcatcgt ggccggactc tccgggatga ttctcttcca
     1381 tttcatgatc aacgtgggca tgaccatggg catcatgccc atcaccggca tacccttgtt
     1441 cttcctgtcc tacggcgggt cctccctgtg ggccgtgctg atcggcatcg ggctgatcct
     1501 ggggatttcc gcccggaggt accactcctg aaccggatag atcccgtcgg actgctgggc
     1561 aagcggctcc tggccgtgga aaagcccgcc cgctacctgg gcggtgagca gggggcggtg
     1621 cgcaaggaag gcagcctgtt cacggtcgcc ctgtgcttcc cggatctcta cgagatcggg
     1681 atgtccaacc acgccctgcg catcctgtac gcggggttga acgccctgcc cggaatccgc
     1741 tgcgagcgcg tgttcgcccc ggcccgggat ttcgaacagg cgcttcgcga aaccggtacg
     1801 cccctgtaca ccctggaaag cggcatcccc ctgtcggagg tcgacctgct ggccgtcacg
     1861 atagggtacg agctttctgc caccaacctc ctgaccgtgc tggatctcgg ggggattccc
     1921 ctggacgcat cctcccgagg gaacggtaat ccgatcgtga tcgccggggg acccgccgtg
     1981 acgaatcccc tgccgatctc ccgtttcctg gacgccgtct ggatagggga ggccgaggcc
     2041 ggcttcttca agctggccgt cgacctggca aacctgggtt ccggagaggc cggacgctcc
     2101 gcccggatcg cgcgccttcg ggaggaaccc gctgtctgga tgcccggcaa gcccgccgtc
     2161 cgtgccgtgg atatggagtt cccggaccgg gaatattccg ggacattccc ctggccggcg
     2221 atcaagcccg tccaggacca cggcaccgtg gagatcatgc ggggctgccc gaacggctgc
     2281 aggttctgcc acgcgggcta cttctaccgg ccccagaggg tacggtcccc cgaacggatc
     2341 ttccgtgagg tcgaatccct gatcctggag tccggatacc gtgagattac cctttcttcc
     2401 ctttcctccg gggattatcc gtgcctcgta gacttggttc gggagctcaa ccgccgctgg
     2461 gcgggtgccc ggatctcctt ccagcttccg tccttgaaag tggagagctt cgacctgtcg
     2521 atcctcgagg agttgaccca tacccgcaaa agcggcctga cgttcgcggt cgagaccccc
     2581 gaggaagcct ggcagagaag catcaacaag tccgtcaccc tggacaagat ccttgcgatc
     2641 ctaggagagg cggcgcatca cggctaccgc ctcgccaagt tctatttcat gatcggtctt
     2701 ccgattcccg gggtctccgg gatcgccgaa accgacgccc tggtcggctt ccttggacgc
     2761 ctgctgaagg aaacccggct cgagttcaac gtcaacatag gcaccttcat acccaagccc
     2821 catactccgt tccagtggtg ttcccagatc ggggaggatg aggcgttttc ccgaatccga
     2881 cgcatccgcg attcgttccg acatgaacgc aggatgaaga tctcctatca cagccccttc
     2941 gtgtccctgc tcgaggggat cctgagccgg ggagacgaga gggtagggga cctcatcctc
     3001 gaggcgtgga gaaacggagc ccgcctggac gcctgggaag accttatgga gaaggacacc
     3061 tggcgacggg ctatgggggg tgttcccgag gcatcgatcc gggaggcact cgacggccgg
     3121 accttggatt ctcctcttcc ttgggactcg atctccctcc gggtcggaaa gggattcctg
     3181 aaacaggaat gggaaaaaac cgcggcttcg accatgacat ccgaatgtac ggacaattgc
     3241 acaaacccgt gcggcgtctg cagggacgac ggatcggctg taataaaaaa catacaacct
     3301 ataatggccg aggaaaggag taccccgatc gggcgacacc ccggggagtc ctccgtgcct
     3361 gtcggatcca agtatcgcct cgtcctgaaa taccggaaga cgggacctgc ggcgtttttg
     3421 ccccatcact ccgtaatgga catgtttcat cgagcgttcc agctggctcg atatcccgtg
     3481 gcgttttccc agggattcaa cccggcccct cgactcgaaa tcgaggaccc gatcccgctg
     3541 ggcatggcgt cccttgagga ctacatttcg gtattgctca cggctcccgt ggacgaaaag
     3601 gataccgtag tcgccttgaa tcgctatctt cctgccgacc tcgcaattca gcgctgtgcc
     3661 atgtacgtga tcccggaggg cgtcaagata cgatcnnnnn nnnnnccggt ccctttcctc
     3721 gatggcctgg gggtctgatt atctggccga gggagttccg ccgaattccg gcgccgctct
     3781 cacggaatcc ttggtccatc ctcggctgga aggttcctgc ctgcttccgt ccggagatgt
     3841 aatcggaact ctgtcgttcc gacttcgtgt cggcggcact cgggaaatcg gattcggctc
     3901 cattttcgaa aaatccttcg gcctgccttt cgccggaagc ccgatacaag ttacccgtct
     3961 gcgacagtgg gcggatatcg gaacggaacc cctggatttc ttcaaggcat tcgattctct
     4021 atacggcagc gcgatttcgt aagcagctag gcatcgaagg acaactgaac atagaagtcg
     4081 acagaatgta cattatagga agcacggggg gggcaagcta ctgcatggaa aagcttaaca
     4141 agctgccgtt aaaccaaatg ggctcactat aaatggactc tactccgtct aaagaaaatc
     4201 cataagataa tacatagcat tggagatatg taagccctgt atccaaatca tactgccctg
     4261 atttcaagtc cgaatcattt tcaacaaaat ctgaatcctt gaaactaaaa aactcagaac
     4321 ttaagttttt cggttttctt gatatcagcg caggaaaacc cggattgatg gaaacgactg
     4381 cgcttgacgg ggcgaagtcg ataaaaacac cggtactatt tctcgtatca aatgatactt
     4441 cgatagagaa tggatcgctt cgctcaggca tattcgtaac atttatgtac ggtgatccgt
     4501 taacaatcaa ttcattataa ccaagattct gaattcgtaa aaatatcgtt gtggggctac
     4561 tgctgaaagc agacgcaatt tgcgttctgc tagaagctgc atcttcacta cttttaaaaa
     4621 atcgatataa gagtatatat ccacgaaata ttgaaatgtc gttacctgta tcgttctcaa
     4681 aatgaagagt ggtaccattc tggatcacat tcctgggagg agcaagataa acataactat
     4741 caattccaca ccctaatatg aaaagcgaaa aaaatggaac tagaacaata atttgcaatg
     4801 atgggataag atggtttatc tttcccacgt tgtccatttt ccccatctca cccctcggcc
     4861 ctgcccttct tccgcggacc cgcggcctcc ggtgccttcg gaggctcaag gggttccagg
     4921 cagtccacga gctcgtagat gccgacaggc ttgttcttcc ccttgacccg gatgttgtcc
     4981 aattcgcgca cgatgaattt gtccttcacg aggccgtagg tgaactcgct tactatgacc
     5041 tgggtcccgt actccttgtt ggtcgcctcc aggcgggagg ccaaattcac gtgatccccg
     5101 gtgagtgtgt agttcatgcg gaggggggat cccatattgc ctaccgtgat gatccccgag
     5161 tttacgccga ttccgacgtt gatgcgcttg ggctcgggcc aggactcgtt gagctccttc
     5221 agccgattca tcatggccag ggcgcacttg cagccccgga cggcgtggtc cggctggggc
     5281 agcggggctc cccagaatcc catgatggca tctccgatgt acttgtccag ggtaccgccg
     5341 taatccatga gcagattggt catggcggtg aaatagacgt tgaggtggtt gacgagctcc
     5401 tggggggcca tgttttcgga gagggtcgtg aagccgcgga tgtccgagaa gaagacggtg
     5461 agctccttgt cctcgccgcc gagctcgg
//
LOCUS       DAOTEI010000209         5437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10840_length_5437_cov_6.071721, whole genome shotgun sequence.
ACCESSION   DAOTEI010000209 DAOTEI010000000
VERSION     DAOTEI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10840_length_5437_cov_6.071721"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="P5313_15080"
     CDS             complement(<1..376)
                     /locus_tag="P5313_15080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY81736.1"
                     /translation="MSAAYGDRDVREIRDDPDRDEYRTLLTSRGLSVPEGSDLVLGLY
                     EGERLAAAGSLVGNVIQGLATARETEGEGSAAVLVTALLKAAADRGLGRVAVFTKPPE
                     AAVFEGLGFRRLAVAERPATGLG"
     gene            complement(381..2306)
                     /locus_tag="P5313_15085"
     CDS             complement(381..2306)
                     /locus_tag="P5313_15085"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRY81737.1"
                     /translation="MAKNLVRKLIDSHLAGKAEGGELSLRVDQTLTQDTTGTMAYLQF
                     EAVGVDRVRTELSVSYVDHNILQTGFENADDHAYLQDVAAKYGIRFSRPGNGICHQVH
                     LERFGVPGKTLLGSDSHTPTAGGLGMLAMGAGGLDVALAMAGVPFSIPDPKVLNVELR
                     GRLSPWVGAKDIILELLRRLSVKGGVGKILEYSGDGVASLTVPERGTITNMGAELGAT
                     TSVFPSDEATRAFLEAQGRGGDWVPLAADPGADYDERIVIDLAALEPLIALPHMPDKV
                     VPVREVEGTPVDQCFIGSCTNSSYLDLVRAAAVLRGRKVAERTSLVIGPGSRQTLLMI
                     TKEGILADLVGAGARILECACGPCIGIGQAPSSGGVSLRTSNRNFEGRSGTKDALVHL
                     VSVETAAASAIRGVITDPRSLGSAPAARQPEAFLVDDSQVVLPPADGSGVRIRRGPNI
                     KALPEFRPLADTLDGEILLTLGDDITTDHILPAGSRIMSLRSNLPAISEYCFSAVDPS
                     FPTRAWAAGGGFVVAGENYGQGSSREHAALSPLFLGVKAVVAVSYARIHRQNLVNAGI
                     LPLTFADKADRDALHAGDRLRIEDARKALDGERIRIRNETTGKTVEALHGLSRRQVEI
                     LRAGGALNAARSPGAGA"
     gene            2451..3161
                     /locus_tag="P5313_15090"
     CDS             2451..3161
                     /locus_tag="P5313_15090"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY81738.1"
                     /translation="MGYLRDVPDLRTQPVRDIIFERLRKAIVQGELKPDTYFTDVEIA
                     EEFGVSRTPVREAVQKLELGGYIERVPMRGNRVLGLSPRELAHSFAIRKALETLALRY
                     TALRISQEDLARMEDILDRLEAFAASGRSEADPEQLFSLVKEYNAVLFRSCGSEHLEG
                     LVYAQREVFDRYGVMRVVLPNRVDRSVSRRRQLWEAFRARDPERAAAIWAEHLDESFA
                     IWRDKSGYAEELKDFPFF"
     gene            3222..4196
                     /locus_tag="P5313_15095"
     CDS             3222..4196
                     /locus_tag="P5313_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRY81739.1"
                     /translation="MKKTLAIVLMLSILAVSFVGAQTWKPTKTIELVAPANPGGGWDM
                     LCRVVQKTLVDEKLVEKNVIVVNKPGGGGAVGWTYLKGKKGQGEFLAATSTLIMLNNL
                     LGSSELTFKDFTPLAALQTEWISVAVQADAPYKNIKDLMEAIKANPAAIPIGVGPTLG
                     NNDHLLILRLAKAYGIDPSKLKFIVYPGAGGEIVPALLGGHIKATTIGLGEVLEQHKA
                     GKLRVLGVSSDAPLSFLPGVQTFKAQGIDVVFPHWRGIIGAPEMTPAMVKYWDDVFTK
                     MVQTKTWKEQIEKLGWTGFYQNSAAHTAFLTEQTGIFNDLLTTVGLKK"
     gene            4278..4754
                     /locus_tag="P5313_15100"
     CDS             4278..4754
                     /locus_tag="P5313_15100"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY81740.1"
                     /translation="MTKDTLTGIIAVAVGGIYTATALVLPEMRMGDKLGPKIFPLVVG
                     IGSVLAGLALILGDRRPGKQSKKADFGFVEHKDLWLKIVLTTVVGIVYGLVMDKLGFL
                     IPTTLFMLFISTLINKGRLVQNAVLSAVFAVITYGVFAVALKLSLPRGFLESLLPF"
     gene            4767..>5437
                     /locus_tag="P5313_15105"
     CDS             4767..>5437
                     /locus_tag="P5313_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY81741.1"
                     /translation="MQEIFANLGLGFQTAFTFTNILWVFIGGTLGTIIGMLPGLGPAT
                     GVAILIPITFGMNPATALITMCAVYYGAMFGGSRASIMINTPGDASAIVSCFDGYPMT
                     KQGRAGAALAISALASFIGGMIGMFFLVFLTQPIAAAALKFGPAEMFSLMMFALVATI
                     TLAEGNLLKGAVAIAFGLMLSTVGIDALTGIIRFTYGAEALQDGIDFLVIIIGVYAVC
                     EVFKN"
BASE COUNT          891 a         1859 c         1790 g          897 t
ORIGIN      
        1 ccccgaggcc cgtggcgggt cgctccgcga cggctaggcg ccggaatccc agtccctcga
       61 agaccgcggc ctccgggggc ttggtgaaga ccgcaaccct gcccaggccc cggtccgcgg
      121 cggccttcag gagggccgtg accaggaccg cggccgagcc ttctccctcg gtctcccggg
      181 ccgtcgccag gccctggatc acgttcccca ccagggatcc cgcggcggcc aggcgctccc
      241 cctcgtacag gcccagtacg aggtccgacc cttcgggtac ggagagtccc cgggaggtca
      301 ggagggtccg gtactcgtcc cggtccgggt cgtcccggat ctcccggacg tcccggtccc
      361 cgtacgccgc ggacacggcc ttaagccccc gcccccgggg agcgggcggc gttcagggcg
      421 ccccccgccc ggaggatctc gacctgccgc cgggacaggc cgtgcagggc ttccacggtc
      481 ttgccggtcg tctcgttccg gatccggatc cgttccccgt ccagggcctt ccgggcgtcc
      541 tcgatgcgca ggcggtcccc ggcgtgcagg gcatcccggt cggccttgtc cgcgaaggtc
      601 aggggcagga taccggcgtt caccaggttc tgccggtgga tccgggcgta ggacacggcg
      661 acgactgcct tgacccccag gaagaggggc gagagggccg cgtgttcccg gctggagccc
      721 tggccgtagt tctccccggc caccacgaaa ccgccccccg cggcccaggc ccgtgtcggg
      781 aaggaggggt ccacggcgga gaaacagtac tccgagatcg ccgggaggtt ggaccggagg
      841 gacatgatcc tgctgcccgc ggggaggata tggtccgtgg tgatgtcgtc ccccaaggtc
      901 aggaggatct ccccgtccag ggtgtccgcc aggggccgga actccggaag ggccttgatg
      961 ttggggcccc ggcggatcct caccccggaa ccgtccgcgg ggggaaggac cacctgggag
     1021 tcgtcgacga ggaaggcctc cggctggcgg gccgccgggg cggagcccag gctccgggga
     1081 tccgtgatca ccccccggat cgcggaggcc gcggcggtct ccacgctgac cagatggacg
     1141 agggcgtcct tcgtaccgga ccggccctcg aaattgcggt tcgaggtacg cagggacacc
     1201 cccccggagc tgggggcctg gccgatgccg atgcagggcc cgcaggcgca ttccaggatt
     1261 cgggctcccg ccccgaccag gtccgccagg atcccctcct tcgtgatcat gagaagggtc
     1321 tgccgggagc ccgggcctat gaccaggctg gtccgctcgg cgaccttccg tccccggagg
     1381 accgcggcgg cacggaccag gtctaggtag gaggagttgg tgcaggaccc tatgaagcac
     1441 tggtccacgg gggtgccctc cacctcccgg acggggacga ccttgtccgg catgtggggc
     1501 agggcgatca ggggctccag ggccgccagg tcgatcacga tcctctcgtc gtagtccgcc
     1561 ccgggatccg cggccagggg aacccagtcc ccgccccggc cctgggcctc caggaaggcc
     1621 cgggtagcct cgtcggaggg gaagaccgag gtggtggccc ccagctcggc gcccatgttg
     1681 gtgatcgtgc cccgctcggg caccgtgagg gaggcgactc catccccgga atactccagg
     1741 atcttcccga ccccgccttt cacggaaagg cgccgcagga gctccaggat gatgtccttg
     1801 gctccgaccc agggggaaag ccggcctcgg agttccacgt tcagaacctt cgggtcgggg
     1861 atcgagaagg ggacccccgc catggccagg gccacgtcca ggccgcccgc gcccatggcc
     1921 agcatcccca ggccccccgc cgtgggggtg tggctgtccg agcccaggag ggtcttcccg
     1981 ggcaccccga aacgctccag gtggacctgg tggcagattc cgttgcccgg ccgggaaaaa
     2041 cggattccgt atttcgcggc cacgtcctgc aggtaggcgt ggtcgtcggc gttctcgaaa
     2101 cccgtctgga ggatgttgtg gtccacgtag ctcacggaca gctccgtgcg gacccggtcc
     2161 accccgaccg cctcgaactg gaggtaggcc atggtgcccg tggtgtcctg ggtcagggtc
     2221 tggtcgaccc gcagggagag ctctcccccc tccgccttgc ccgccaggtg ggaatcgatg
     2281 agtttccgga ccaggttctt cgccataact cctcctaatg gaagaatcgc gggatttccc
     2341 gcgaattccg ctttacagat tccgaatgcg ggtctagtat acacctggat tctggatcca
     2401 ggatgcagaa tccacaaaat ttgtaaagat ttttgtccag gagggtcgtc gtgggttatc
     2461 tgagggatgt tccggacctc cgcacccagc cggtacggga catcatcttc gagcgcctcc
     2521 ggaaggccat cgtccagggc gagctgaagc cggacaccta cttcacggac gtggagatcg
     2581 cggaggagtt cggcgtcagc cggacccccg tgcgggaggc ggttcagaag ctggaactcg
     2641 ggggctacat cgagcgggtg cccatgcggg gcaaccgggt cctcggactg tcgccccggg
     2701 agctggccca ctccttcgcg atccgcaagg ccctggagac cttggccctc cggtacacgg
     2761 ccctgcgcat ctcccaggaa gacctggcac ggatggagga catcctggac cggctggaag
     2821 ccttcgccgc ctccggacgg agcgaagcgg acccggagca gctcttctcc ctggtgaagg
     2881 agtacaacgc ggtcctgttc cggtcctgcg gttccgagca cctcgagggc ctggtctacg
     2941 cccagcggga ggtgttcgac cggtacgggg tcatgcgggt ggtcctgccc aaccgggtgg
     3001 accgcagcgt gtcccggcga aggcagctct gggaggcttt ccgggcccgg gatcccgagc
     3061 gggccgcggc gatctgggcg gagcacctgg acgaatcctt cgcgatctgg cgggacaagt
     3121 cgggctacgc ggaagagctc aaggactttc ctttctttta ggccgcatcc gccccagtag
     3181 gcggtcgagc gatatttccg atccttagga ggaacggtat catgaagaag acactcgcga
     3241 tcgtgcttat gctctcgatc ctggcggtgt ccttcgtcgg cgcccagacc tggaagccca
     3301 cgaagaccat cgagctggtg gcacccgcca acccgggcgg cggctgggac atgctctgcc
     3361 gggtcgtgca gaagaccctg gtggacgaga agctcgtcga gaagaacgtg atcgtggtca
     3421 acaagccggg cggcggcggg gccgtgggct ggacctacct gaagggcaag aaggggcagg
     3481 gggaattcct ggccgccacc tccaccctga tcatgctgaa caacctcctg ggctccagcg
     3541 agctgacctt caaggacttc acccccctgg ccgccctgca gaccgagtgg atctccgtgg
     3601 ccgtccaggc ggacgccccc tacaagaaca tcaaggacct gatggaggcc atcaaggcca
     3661 accccgcggc catccccatc ggcgtcggcc ccaccctggg caacaacgac cacctcctga
     3721 tcctccgcct ggccaaggcc tacgggatcg acccctccaa gctcaagttc atcgtctacc
     3781 ccggcgccgg cggggagatc gtcccggccc tcctgggcgg gcacatcaag gccaccacca
     3841 tcggcctggg cgaggtcctg gagcagcaca aggccggcaa gctccgggtc ctgggcgtca
     3901 gctccgacgc tcccctgtcc ttcctgcccg gagtccagac cttcaaggcc cagggtatcg
     3961 acgtggtctt cccccactgg cgcggcatca tcggcgcccc ggagatgacc cccgccatgg
     4021 tcaagtactg ggacgacgtc tttaccaaga tggtccagac caagacctgg aaggaacaga
     4081 tcgagaagct cggctggacc gggttctacc agaactccgc ggcccacacg gccttcctga
     4141 ccgagcagac agggatcttc aacgacctgc tcaccacggt cggactcaag aagtaattcc
     4201 gtaaagtagg caaagcgggg gcgggggaaa tcccgtcccc gcgaaggcgc gtccgggacg
     4261 cgccgggagg tgcgctcgtg acgaaagaca ccctgaccgg aatcatcgcc gtggccgtgg
     4321 gaggtatcta cacggccacc gccctggtcc tgcccgagat gcggatgggg gacaagctcg
     4381 ggcccaagat ctttcccctg gtggtcggca tcggatccgt cctggcgggc ttggccctga
     4441 tcctggggga ccgcaggccc ggtaagcagt ccaagaaggc ggacttcggt ttcgtggagc
     4501 acaaggacct gtggctcaag atcgtcctga ccacggtggt cgggatcgtc tacggcctgg
     4561 tgatggacaa gctgggcttc ctgatcccta cgaccctctt catgctcttc atctccaccc
     4621 tcatcaacaa aggccggctg gtccagaacg cggtcctctc ggccgtgttc gcggtcatca
     4681 cctacggggt cttcgcggtg gccctgaaac taagcctgcc ccggggattc ctggaatccc
     4741 tgctgccctt ctaggaggag accgagatgc aagagatatt cgccaacctc ggtctgggat
     4801 tccagaccgc gttcaccttc acgaacatcc tgtgggtgtt catcggaggg accctgggaa
     4861 cgatcatcgg catgctcccg ggactcgggc cggccacggg ggtggccatc ctgatcccca
     4921 tcaccttcgg catgaacccc gccacggccc tcatcacgat gtgcgcggtg tactacgggg
     4981 ccatgttcgg aggctcccgg gcttccatca tgatcaacac ccccggggac gcctcggcca
     5041 tcgtgtcctg cttcgacggc taccccatga ccaagcaggg acgcgccggc gcggccctgg
     5101 ccatctccgc cttggcctcc ttcatcggcg gcatgatcgg catgttcttc ctggtcttcc
     5161 tgacccagcc catcgcggcc gcggccctga aattcggacc cgcggagatg ttctccctca
     5221 tgatgttcgc cctggtggcc acgatcaccc tggcggaagg caacctcctc aaaggtgcgg
     5281 tggccatcgc cttcggcctc atgctctcca cggtgggcat cgacgccctg acggggatca
     5341 tccgcttcac ctacggggcg gaggccctcc aggacgggat cgacttcctg gtcatcatca
     5401 tcggtgtcta cgcggtctgc gaagtcttca agaactt
//
LOCUS       DAOTEI010000210         5426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10868_length_5426_cov_4.598026, whole genome shotgun sequence.
ACCESSION   DAOTEI010000210 DAOTEI010000000
VERSION     DAOTEI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10868_length_5426_cov_4.598026"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="P5313_15110"
     CDS             <1..377
                     /locus_tag="P5313_15110"
                     /inference="COORDINATES: protein motif:HMM:NF024278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HRY81742.1"
                     /translation="PKPFVAACDKFIYTEILREADEEAVPEKAKPEKAKEVVAADWKN
                     DRKLQAMLRAAVEDAADESGWAYLGPVGQYVANRQPEFDPRNYGFRKLGELIRSSGWF
                     EIDERTNAVDPGKQVFIRYKKR"
     gene            complement(451..792)
                     /locus_tag="P5313_15115"
     CDS             complement(451..792)
                     /locus_tag="P5313_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY81743.1"
                     /translation="MAELQAVFIQNLKKERGRLRISQSTLAERAELSSGFIGEIESGR
                     KFPSIDTVERLARALEVPPYRLYMSDRDLEDLLAPEGKPAWAKAALVRWAAEMLGKYA
                     GEPNDRSRSPD"
     gene            875..1180
                     /locus_tag="P5313_15120"
     CDS             875..1180
                     /locus_tag="P5313_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81744.1"
                     /translation="MARIRARPRPRTSSEALRALSLADLDTAPGDPRDCRVWESVHLG
                     RDGRILLSPRRLGGTWTPPGYAACLRRLRLLLRREGFPAGFLGPKKDRLRAEKYRRL"
     gene            complement(1152..2153)
                     /locus_tag="P5313_15125"
     CDS             complement(1152..2153)
                     /locus_tag="P5313_15125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY81745.1"
                     /translation="MATLRLRLTLAAAAVLLVSCATAPRGSPLPEAPPDPASAPALRV
                     EVDGIPIHAFDQGPEDADILVLGVHGWGASGAEGLAFGRALGREAVSAGAADSRAPTV
                     RLVSPDLPGSGASGKPEAPYTPEWFVAILDGLREALRRDRVVLAGHSLGGRLALEYTL
                     AHPERVEALILLAPAGLSDSFYPLDRWALGRDSIIRAGTRLTTERLYMRFYRKRVVRD
                     PRYAYPDVAAWAVAGLLTPEGRSALREVTRHALAAPDLTDRLDEIEVPVLLVWGRDDE
                     VLRFESSAAYRELLPDLRGFLALEDCGHHPQTERSARTARAAYEFLAGLRAAGTSPP"
     gene            complement(2129..2989)
                     /locus_tag="P5313_15130"
     CDS             complement(2129..2989)
                     /locus_tag="P5313_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY81746.1"
                     /translation="MKILARDEWSAERPLPLSSEGSLRLFFVGAGSAFSKKFYQTNLL
                     VTKGDTHLLIDCGTRTPEALAFLGLPVTRIDNFLITHSHADHVGGLEEVMLMNRYGPK
                     KKPSIVISRAYQRLLWSASLRGGCAYNERHDDRPLEFEDFWVPIRPKRIPGADRQLSI
                     ARVGSLRLTLFRTKHIPDNSASWRDSSPSHGVILDDRVLFTSDTRYDPGMVLDLDRTY
                     RFEAIFHDGQLFTGGVHASMEELSALPAEVKSRMYLMHYQDSVSSQVERAKDLGFAGF
                     SEQWRHYVFD"
     gene            3081..3890
                     /locus_tag="P5313_15135"
     CDS             3081..3890
                     /locus_tag="P5313_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019855904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY81747.1"
                     /translation="MSRKVWFITGASKGFGRIWAEAALARGDRVAAAARNPETLRDLA
                     AAYGDSILPLALDVRDRSAAFRAVAEARARFGRLDVVVNNAGYGLFGAVEDVTEEQAR
                     AQMETNFFGTLWVTQAAVPVLRDQGGGRILQVTSLGGVVSFPFLGLYHASKWAVEGMS
                     ESLAKEVAGFGIRITLIEPGPFGTDWRGPSAAHAPKSAPYQGAGPVGSGLKSGDPKAT
                     AAAILRIADSEDPPLRIFLGAGVAELARGVYDERRKTWDAWAEVSTAAQGS"
     gene            3992..4420
                     /locus_tag="P5313_15140"
     CDS             3992..4420
                     /locus_tag="P5313_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81748.1"
                     /translation="MTEDAEAFWRDYERSTGETVEARAMGQWLGPGAPREGVWGILIL
                     TDRSFHFLGRKSENWLKGIFTLRSRAEGEAVEFRVPREDLRSLEAPDRGWLSRILGSP
                     FPEVTLTWNPAGGGEPVRAVFNVDDPQGFLAKLGAAAGAR"
     gene            4513..>5426
                     /locus_tag="P5313_15145"
     CDS             4513..>5426
                     /locus_tag="P5313_15145"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY81749.1"
                     /translation="MSTLKRYKKLENTLVQRLVAALTRAASASRDFFRRFMKAGRQKL
                     TIMLVPHTEKRILNLQLSFFGLAGILLAVGIVLSVSVYATSRFGSTARLLSGKVSDLR
                     STQADLDAVRDETTTLIKAARNFEAALSSTLSRIGIQPSSSSTADRDGDLAAFFDVEE
                     AARGSLREVGEIRKVTRYLEKTVSAVKELGGVVASQSEVLSEIPNLWPVKGGIGHISM
                     YYGQNENPFYGSWYLHKGLDISTFRMGDSVVATADGKVANIGYDPSYGNNIILEHKHG
                     FFTRYAHLQSVRVQKGQKVQQGQVIGYLG"
BASE COUNT          849 a         1790 c         1937 g          850 t
ORIGIN      
        1 cccccaagcc cttcgtggcg gcctgcgaca agttcatcta caccgagatc ctgcgggagg
       61 cggacgagga ggcggttccg gagaaggcca agcccgagaa ggccaaggag gtggtggccg
      121 cggactggaa gaacgaccgc aagctccagg ccatgctccg ggccgcggtg gaggacgccg
      181 cggacgagtc cggctgggcc tacctgggac ccgtcgggca gtacgtggcc aaccggcagc
      241 ccgagttcga cccccgcaac tacggcttcc gcaagctcgg cgagctcatc cggtcctccg
      301 gctggttcga gatcgacgag cgcacgaacg ccgtggatcc cgggaaacag gtgttcatcc
      361 ggtacaagaa gaggtaacag gacacaggaa ccaggactca gggccttggc ggaagacggc
      421 ggatacgttc aggtgtccgt cgccctagta tcaatcgggt gaccggctcc ggtcgttcgg
      481 ctcccccgcg tactttccca gcatctccgc cgcccagcgg accagggcgg ccttggccca
      541 ggcgggcttc ccttcggggg cgagcaggtc ctccaggtcc cggtcggaca tgtacaggcg
      601 gtagggcggc acctccaggg cccgggccag gcgctccacg gtgtcgatgg aggggaactt
      661 gcggccgctc tcgatctccc cgatgaaccc ggaggagagt tcggcgcgct cggccagcgt
      721 cgattgggag atcctcaggc ggcctcgctc cttcttgagg ttctggatga atacggcctg
      781 gagttccgcc acgggatcgt ctcccttcgg tggattgatg ctagccccgg gccccttgct
      841 tgacaatttg ctgatagcga gtcgatcctg gcttatggcc aggattcgag cccgtcctcg
      901 tccccggacc tcttccgaag ccctccgggc cctgagcctg gcggatctcg acacggcccc
      961 gggggatccc cgggactgcc gggtgtggga gtccgtccac ctgggccggg acggaaggat
     1021 cctcctatcg ccccggcggc tgggcgggac ctggacgccc ccgggctatg ccgcctgcct
     1081 gcgccggctg cggcttctcc tgaggcgcga aggatttccg gcggggttcc tgggtccgaa
     1141 gaaagaccgg ctcagggcgg agaagtaccg gcggctctga gcccggccag gaattcgtag
     1201 gccgcccggg cggtccgggc ggaacgttcc gtctgggggt ggtggccgca gtcctccaag
     1261 gccaggaagc cccggaggtc gggtaggagc tcccggtacg ccgcggagga ctcgaagcga
     1321 agcacctcgt cgtccctccc ccagaccagg agcacgggca cctcgatctc gtccaaccga
     1381 tccgtcaggt ccggggcggc cagggcgtgc cgggtcacct cgcgcagggc gctccgcccc
     1441 tcgggggtga ggagcccggc tacggcccag gcggccacgt ccggataggc gtaccgggga
     1501 tcccgcacca cccgcttccg gtagaacctc atgtacagcc gctcggtggt cagtctcgtc
     1561 cccgcgcgga tgatcgagtc ccggcccagg gcccaacggt ccagggggta gaaggagtcc
     1621 gacagccccg cgggggccag gaggatcagg gcctccaccc gctcaggatg ggccagggtg
     1681 tattccaggg ccaggcggcc tcccagggag tgcccggcca ggaccacccg gtccctcctc
     1741 agggcttccc ggaggccgtc caggatcgcg acgaaccatt ccggggtgta gggtgcctcg
     1801 ggcttgccgg aggcaccgga gccgggcaga tccggggaca cgagccgcac cgtcggggcc
     1861 cgggaatccg cggcccccgc ggagaccgcc tcccgcccca gggcccgtcc gaaagccagg
     1921 ccctcggccc cggaggcccc ccatccgtgg acccccagga ccaggatgtc cgcgtcctcg
     1981 ggaccctggt cgaaggcgtg gatcgggatt ccgtccacct cgacccggag ggccggggcg
     2041 gacgcggggt ccggcggagc ctccgggagg gggctgcccc ggggggcggt cgcgcaggag
     2101 accaggagga cggcggccgc cgccagggtc agtcgaagac gtagtgtcgc cattgctccg
     2161 agaaccccgc gaacccgagg tcctttgccc gttcgacctg ggaggagacg gaatcctggt
     2221 agtgcatgag gtacatgcgg gatttcacct ccgccggtag ggcggacagc tcctccatcg
     2281 aggcgtgcac gcccccggtg aagagctggc cgtcgtggaa gatcgcctcg aagcggtagg
     2341 ttcggtcgag gtccaggacc atgccggggt cgtagcgggt gtcgctggtg aacaggaccc
     2401 ggtcgtcgag gatcaccccg tggctggggg aggagtccct ccaggaggcg gagttgtcgg
     2461 ggatgtgctt ggtgcggaac agggtgagcc gcagggagcc tacccgggct atggaaagct
     2521 ggcggtccgc cccggggatc ctcttggggc ggatggggac ccagaagtcc tcgaactcca
     2581 gcgggcggtc gtcgtgccgc tcgttgtagg cgcagcctcc ccggagggac gcgctccaca
     2641 ggaggcgctg gtaggcccgg gagataacga tggaaggttt cttctttggg ccgtagcggt
     2701 tcatgagcat gacctcttcc aggccgccga catggtccgc gtgggagtgg gtgatcagga
     2761 aattgtcgat cctggtaacc gggagcccca ggaaggccag ggcctcgggg gtccgggtgc
     2821 cgcagtcgat cagcaggtgg gtgtcgccct tggtcacgag gaggttcgtc tggtagaatt
     2881 tcttggagaa ggcgcttccc gccccgacga agaagagccg gaggctcccc tcgctcgaaa
     2941 gcggaagggg ccgttccgcg ctccactcat cccgggccag tattttcatc cacttaacta
     3001 tatcgaaggg tcccggggtt ttcaatgaag gggcgaattg tagtatattg cgtcgtatcc
     3061 cttccttagg agctgcatcg atgtcgcgga aagtctggtt catcaccggc gcgtccaagg
     3121 gtttcgggcg gatctgggcg gaggcggccc tggcccgggg agaccgggtg gcggcggccg
     3181 cccgcaatcc ggagaccctg cgggacctcg ccgccgccta cggggactcc atcctgcccc
     3241 tggccctgga cgtgcgggac cggtccgcgg cgttccgggc cgtggccgag gcccgggccc
     3301 gcttcgggcg gctggacgtg gtcgtgaaca acgcgggcta cggcctgttc ggcgccgtgg
     3361 aggacgtgac ggaggagcag gcccgggccc agatggagac gaacttcttc ggtaccctgt
     3421 gggtgaccca ggcggccgtg ccggtccttc gggaccaggg cggcggccgg atcctccagg
     3481 tgaccagcct ggggggcgtc gtgtccttcc ccttcctggg gctctaccac gcctccaagt
     3541 gggccgtgga ggggatgagc gagtccctgg ccaaggaggt cgcgggcttc gggatccgga
     3601 tcacccttat cgagccgggg cccttcggca cggactggcg gggtccctcc gcggcgcacg
     3661 ccccgaagtc ggccccctac cagggcgccg ggcccgtcgg gtcgggactg aagtccggcg
     3721 acccgaaggc cacggccgcg gcgatccttc ggatcgcgga ctccgaggat ccgcccctgc
     3781 ggatcttcct gggcgccggg gtggccgaac tcgcccgggg ggtctacgac gaacgccgga
     3841 agacctggga cgcctgggcc gaggtatcga cggccgccca gggaagctga gaatccggcg
     3901 gccccggcca cggcttcggc cgcggccccg ggccgccgcg gcggttccgc ggcgcggggt
     3961 actccgcgcc gccttgatga tgggaggcga tatgaccgag gatgccgagg cgttctggag
     4021 ggactacgag cgctcgaccg gggagaccgt ggaggcccgg gccatgggcc agtggctggg
     4081 gccgggggcc ccccgggaag gggtgtgggg cattctgatc ctcacggacc gcagcttcca
     4141 cttcctgggt cgcaagtccg agaactggct taaggggatc ttcaccctga gaagccgggc
     4201 ggagggggag gccgtggagt tccgggtgcc ccgggaggat ctccggtccc tggaggcgcc
     4261 cgatcgaggc tggctgtccc ggatcctggg gtcccccttc ccggaagtca cccttacctg
     4321 gaatcccgcc gggggagggg aacccgtccg tgcggtgttc aacgtggacg acccgcaggg
     4381 cttcctggcc aagctgggcg ctgcggcggg cgcccggtag cccgggcgat cttttcaaat
     4441 gcctttgaca aaccgataat ctaggtgcta aactgatccg atagctatct tcgcgagccc
     4501 taaggagcgc gggtgtcgac tcttaaacga tataagaagc tggagaatac tctggttcag
     4561 cggctggttg ccgccttgac ccgggcggcc tccgcttccc gggatttttt ccgccgattc
     4621 atgaaggccg gccggcagaa actcaccatc atgctggtcc cccacacgga aaagcgtatt
     4681 ctgaacctcc aactcagctt cttcggcctg gcgggcatcc tcctggccgt gggcatcgtc
     4741 ctgtccgtat cggtgtacgc cacctcccgc ttcggcagca ccgcccggct cctgtcgggc
     4801 aaggtctcgg acctccgctc cacccaggcg gacctggacg cggtccggga cgagaccacg
     4861 accctgatca aggcggcccg caacttcgaa gccgccctgt cctccaccct gtcccgcatc
     4921 ggcatccagc cctcctcgtc ctccactgcg gatcgggacg gagacctggc ggccttcttc
     4981 gacgtggagg aagcggcccg gggctccctg cgggaggtgg gggagatccg gaaggtgacc
     5041 cgctacctgg agaagacggt cagcgccgtc aaggagttgg gcggggtcgt ggcctcccag
     5101 agcgaggtcc tctcggagat accgaacctc tggcccgtga agggcgggat cggccacatc
     5161 tccatgtact acggccagaa cgagaaccca ttctacgggt cctggtacct gcacaagggc
     5221 ctggacatct ccaccttccg gatgggggac tccgtcgtgg ccaccgcgga cggcaaggtg
     5281 gccaacatcg ggtacgaccc cagctacggc aacaacatca tcctggagca caagcacggg
     5341 ttcttcaccc gctacgccca cctccagtcc gtccgggtcc agaagggcca gaaggtccag
     5401 cagggacagg tcatcggcta cctggg
//
LOCUS       DAOTEI010000211         5415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_10893_length_5415_cov_4.762127, whole genome shotgun sequence.
ACCESSION   DAOTEI010000211 DAOTEI010000000
VERSION     DAOTEI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10893_length_5415_cov_4.762127"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="P5313_15150"
     CDS             <1..889
                     /locus_tag="P5313_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY81750.1"
                     /translation="VSMPSATHGWMGNANWWAQRAEKDWEARDKAVQIELKFSGNVNQ
                     QVADIEDLLVKKVDALVVFPHDTSVTSIVEKAFNAGVYVVVLDRGVSKEGIYDIYITN
                     DDEAYTRKGMEWLAKQMGFKGNLLLITGIPSPIDTIRTDTIKEVVKKYPNIKLLDVQP
                     GDWNRQKSLTVMENYLQKYKQIDAVYTADDDMMLGAMQAYKESGRKDIKLFLGGGCLK
                     SVIKEIMDDSNPLVKADVTYSPSVIATSISYAVIGVKGEKLNPNVYQVRALPRRIILP
                     SELVTRENAKDFYVPEAPF"
     gene            1190..2278
                     /locus_tag="P5313_15155"
     CDS             1190..2278
                     /locus_tag="P5313_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY81751.1"
                     /translation="MGFKVGIVGAGGMTRYHVPGFRSAGVEVVGLADPVPEARERTVK
                     LHGIPAAYASLDDLLAAHKDVDIISILTPNKFHAPLAIQALRAGKHVFCEKPPALDAA
                     STEQMAAEAKKAGKRLQFNFNNRARPESFAMMEYIRSGEVGRINSAQAVWCRRSGVPG
                     FGGWFTDKSVSGGGPVIDLLHMLDLALHFLGYPEPEWVLAQTFRDFIDNRDFRGPWGI
                     PDVPGGKIDVEAACHGFIRFRTGQVCFVRSSWAEMSERETVSVTFQGTKAGGVVERLF
                     GVDGIDETSVDRCQLFTVEHGRHVNRNVIVPRDDAMGRLRSVQNFVRSLEGTEEPLNT
                     PDQAVKLMKVVDALFASSASGEPVRVAR"
     gene            2374..3372
                     /locus_tag="P5313_15160"
     CDS             2374..3372
                     /locus_tag="P5313_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY81752.1"
                     /translation="MEHKICVRIKSGEYVEALDLSRARRAVLHLTTIEDFQTAAEIPL
                     YLVSGTERKPLATLSAERLSPLSSEECRLDLQTSLVGRRRAEFRVVQNGRLVLTETCG
                     LPIPGPGRRILAVAAILAAAAGLTLLVLFSIRGCAADGGPGGIGARVEAPSAPAVKPR
                     APSAPASPASTEVAAPAAQGTAPEAAAVSAAPQTSPPPTPAPVQAPVRTDPLEAAREA
                     FTALGEETLTVYFSADSAGILRDQRDKLGKVAAVLVRYPDLGITISGHCATAGTEKGR
                     LALSQDRARAVYEALAAAGWKPKTATAVEGFGDERPATRAEDRQALNRRVEIRLAP"
     gene            complement(3404..4522)
                     /locus_tag="P5313_15165"
     CDS             complement(3404..4522)
                     /locus_tag="P5313_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81753.1"
                     /translation="MNRLFDRTRIRVKPLAERVNKLDIERDAVDPSSYEGSLSEAARA
                     DAAAAAREAREARARGAPVILAFGAHSIKNGLSRLMIRLMEGGRVTHFATNGAGIIHD
                     WEFAFQGRSGEDVRRYVAEGQFGIWEETGSCLNLALAVGAWRGLGYGESVGALVAEGG
                     LPIPSDAELADAVLAGAAGDADETVLGRAAAAADLRALLRAHPLPAGFLRVEHPWARY
                     CLSAAAYRAGVPFTCHPMFGHDIIYTHPAARGAPIGRTAELDFLSFADSVSRLEGGVY
                     LSVGSAVMSPMIFEKALSMARNTARAENRRIEDFSIHVVDLAESRWDWQADGEPPQDN
                     PAYYLRFCKTFSRMGGRMTYAAADNRAWLTEFLRELEA"
     gene            complement(4519..>5415)
                     /locus_tag="P5313_15170"
     CDS             complement(4519..>5415)
                     /locus_tag="P5313_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY81754.1"
                     /translation="YGGLPHWGVDLGAAGTVVNNLAALGTGRLVAVGFTGDDGEGWEL
                     RQDLAALGVDRTHLHTDPARRTPLYLKPRDAGRPGLAGEHSRYDVKNRSATSPDLEAA
                     ILRSLRAVLPESDALIVMDQVEEEGRGAVTAAVVRALADLLPRFPRVVAWADSRRRIH
                     AYRKMILKMNQFELAGVHDPEPGSEIPEGRIVAALAETEARAGAGVYATVGEHGVWVG
                     GKRPVRVPAVLVPPPVDPTGAGDSFSAGAVLALASGASPVEAALVGNLVASITVRQLS
                     TTGTARPEELPGALELWKEQNP"
BASE COUNT          898 a         1865 c         1817 g          835 t
ORIGIN      
        1 cgtgtccatg cccagcgcca cccacgggtg gatgggcaac gcgaactggt gggcccagag
       61 ggccgagaag gactgggagg cccgggacaa ggccgtccag atcgagctca agttctccgg
      121 caacgtgaac cagcaggtgg cggacatcga ggacctcctg gtgaagaagg tggatgccct
      181 ggtggtcttc ccccacgaca cctcggtcac cagcatcgtc gagaaggcct tcaacgcggg
      241 cgtgtacgtg gtcgtcctgg accgcggcgt ctccaaggaa ggcatctacg acatctacat
      301 caccaacgac gacgaggcct acacccgcaa gggcatggaa tggctggcca agcagatggg
      361 cttcaagggc aacctgctcc tgatcacggg catcccctcc ccgatcgaca ccatccgcac
      421 ggacacgatc aaggaagtgg tgaagaagta tcccaacatc aagctgctgg acgtccagcc
      481 cggcgactgg aaccgccaga agtccctgac cgtcatggag aactacctgc agaagtacaa
      541 gcagatcgac gccgtgtaca ccgcggacga cgacatgatg ctgggcgcca tgcaggcgta
      601 caaggagtcc ggccgcaagg acataaagtt attcctgggc ggcggctgcc tcaagagcgt
      661 gatcaaggag atcatggacg actccaaccc cctggtgaag gcggacgtca cctactcccc
      721 ctcggtcatc gccacttcca tctcctacgc ggtcatcggg gttaagggcg agaagctcaa
      781 cccgaacgtc taccaggtcc gcgcccttcc gcgccggatc atcctgccct ccgagctcgt
      841 gacccgcgag aacgccaagg acttctacgt ccccgaggcg ccgttctgag aaactgaacg
      901 taataaaaaa ttgaaccaca gggtttaatt aaaaattgaa ccacagaggc acagaggcac
      961 agagataaat gagggattga ggataggagg agacaggata acaggataga gacgggatac
     1021 acaggatgga tcttgggaaa atcgactccc tgattaatat cctgttaatc cttcataatc
     1081 ctgttcatcc tgtctctctt ccggttccat gaccgttctc tctgtgctcc tctgtgctct
     1141 ctgtgcctct gtggttgttt ttcggaatag ttgttcgagg aggacgggca tgggattcaa
     1201 ggtcggcatc gtaggcgccg ggggcatgac gaggtaccac gttcccgggt tccggagcgc
     1261 gggggtcgag gtcgtagggt tggcggatcc cgtccccgag gcgagggaac ggacggtcaa
     1321 gctccacgga atcccggccg cgtacgcgag cctggacgat cttttggcgg ctcacaaaga
     1381 cgtcgatatc atttccatcc tcacccccaa caagttccac gcccccctgg ccatccaggc
     1441 cctgcgggcg ggcaagcacg tcttctgcga gaagcccccg gccctggacg ccgcttccac
     1501 ggaacagatg gccgcggagg cgaagaaggc ggggaaacgt ctccagttca acttcaacaa
     1561 ccgcgcccgc cccgagtcct tcgcgatgat ggagtacatc cgctccggcg aggtcggccg
     1621 gatcaactcg gcccaggcgg tctggtgccg gcgcagcggg gtccccggct tcggcggctg
     1681 gttcacggac aagtccgtct cggggggcgg tcctgtgatc gacctgctgc acatgctgga
     1741 cctggccctc cacttcctgg gctatccgga gcccgagtgg gtcctggccc agaccttccg
     1801 ggacttcatc gacaaccggg acttccgggg cccctggggc atccccgacg tgccgggcgg
     1861 caagatcgac gtcgaggcgg cctgccacgg cttcatccgg ttccggacgg gtcaggtctg
     1921 cttcgtccgg tcctcctggg cggagatgtc cgagcgggag accgtgtccg tgaccttcca
     1981 gggtaccaag gcgggaggtg tggtggagcg cctgttcggc gtggacggga tcgacgagac
     2041 cagcgtggac cgctgtcagc tcttcaccgt ggaacacggc cgccacgtca accggaacgt
     2101 gatcgtgccc cgggacgacg ccatgggccg gctccgctcg gtccagaact tcgtgcgctc
     2161 cctggagggg accgaggagc ccctgaacac cccggatcag gcggtcaagc tgatgaaggt
     2221 cgtggacgcc ctcttcgcat cctccgcctc gggagagccg gtgagggtgg cgaggtaggg
     2281 gcggatcccc gacgcggccg ccccgcccgg ttcgggcgga agtttttttc cccgcttctt
     2341 gacccgccgg gaattggggt tactatctta cgtatggagc acaaaatctg cgtgcggatc
     2401 aagtccggcg agtatgtcga ggccctggac ctctcccggg cccgccgcgc cgtactccac
     2461 ctgacgacca tcgaggactt ccagaccgca gcggagatcc ccctctatct cgtctccggc
     2521 acggagcgca aacccctagc caccttgagc gccgagcggc tttccccgct ttcctccgag
     2581 gagtgccgcc tggatctgca gaccagcctc gtgggacgcc gccgggcgga attccgggtg
     2641 gtccagaacg gacggcttgt cctgaccgag acctgcggtc ttccgatacc cggacccggc
     2701 cgccggatcc tcgccgtcgc cgcgatcctg gcggccgccg cgggtctgac cctgctcgtt
     2761 cttttctcca tccggggctg cgccgcggac ggggggcccg gcgggatcgg cgcacgggtc
     2821 gaagcccctt cggcgcccgc cgtcaaaccc cgggccccgt cggctccggc atcccccgca
     2881 tcgacggagg tcgcggcccc ggccgcccaa ggcaccgccc ccgaggctgc cgcagtttcc
     2941 gccgctcccc agacctcccc cccgcctacc ccggcgcccg ttcaagcgcc cgttcgtacg
     3001 gatcccctcg aagccgcccg ggaggccttc acggcgctcg gggaggaaac cctgaccgtc
     3061 tatttctccg cggattccgc ggggatcctc cgggaccagc gggacaagct cgggaaggtg
     3121 gcggccgtcc tggtccggta tccggacctg gggatcacga tctccggcca ctgcgccacc
     3181 gcgggcaccg aaaagggaag gctggccctg tcccaggacc gggcccgggc ggtgtacgag
     3241 gccctcgcgg cggcggggtg gaagccgaag acggcgacgg ccgtcgaggg cttcggggac
     3301 gagagacccg ccacccgggc ggaggaccgg caggccctca accgccgggt ggagatccgc
     3361 ctggcgccct gatactcctg ttctgaatgg gcggtaagat tctctatgct tccagctccc
     3421 gaagaaactc cgtaagccag gcgcggttgt ccgcggcggc gtaggtcatg cggcccccca
     3481 tccggctgaa ggtcttgcag aaccgcaggt agtaggccgg attgtcctgg ggcggctctc
     3541 cgtcggcctg ccagtcccag cgggactccg ccaggtccac cacgtggatg gagaagtcct
     3601 cgatccggcg gttctccgcg cgggccgtgt tccgggccat ggacagggcc ttctcgaaga
     3661 tcatggggga catgaccgcg gagcccacgg agaggtagac ccctccctcc agccgggaga
     3721 ccgagtcggc gaaggacagg aaatcgagct ccgcggtccg gccgatcggg gcgccccggg
     3781 cggcgggatg ggtgtagatg atgtcgtggc cgaacatggg gtggcaggtg aagggcaccc
     3841 ccgcgcggta cgccgccgcg gacagacagt accgggccca gggatgctcc acccgcagga
     3901 agcccgcagg aagcggatgg gcccggagga gggcccggag gtccgccgcc gccgccgccc
     3961 ggcccaagac cgtctcgtcc gcgtcccccg cggccccggc caggacggcg tccgcgagtt
     4021 ccgcgtccga ggggatcgga aggcccccct cggcgaccag ggcgcccacg gactcgccgt
     4081 aacccaggcc ccgccaggcc ccgaccgcca gggccaggtt gagacaggac ccggtttcct
     4141 cccagatccc gaactgcccc tccgccacgt aacggcgcac gtcctccccg gaccttccct
     4201 ggaaggcgaa ctcccagtcg tggatgatcc ccgccccgtt cgtggcgaag tgggtgaccc
     4261 ggccgccctc catgaggcgg atcatcaggc gggaaagccc gttcttgatc gagtgggccc
     4321 cgaaggccag gatgaccggg gctccccggg cgcgggcttc ccgggcttcc cgggccgcgg
     4381 ccgcggcgtc cgcccgggct gcctccgaga gggatccctc gtaggaggac gggtccacgg
     4441 cgtcccgctc gatgtccagc ttgttgaccc gttcggccag gggcttcacg cggatccggg
     4501 tgcggtcgaa gagtcggttc acgggttctg ctccttccag agctccaggg ctccgggcag
     4561 ctcctccggc cgggcggtgc ccgtggtgga gagctgtcgg acggtgatcg aggcgaccag
     4621 gttgccgacc agggctgcct ccacggggga ggccccggaa gccagggcca ggacggctcc
     4681 cgcggaaaag gaatcccccg cgccggtggg atccacgggg ggcggcacga ggacggcggg
     4741 cacccggacg gggcgcttgc cgccgaccca gaccccgtgt tcgcccacgg tggcgtagac
     4801 ccccgcgcct gcccgggctt ccgtctccgc cagggccgcg acgatgcggc cttccgggat
     4861 ctccgagccg ggttcggggt cgtggacgcc ggccagctcg aactggttca tcttgaggat
     4921 catcttccgg taggcgtgga tccgccttcg ggagtcggcc caggccacga cgcgggggaa
     4981 cctcggaagg aggtccgcca gggctcggac caccgcggcc gtcacggccc cccggccctc
     5041 ttcctccacc tggtccatca cgatcagggc gtccgactcc ggaaggaccg cccggaggga
     5101 gcggaggatc gcggcctcga gatccgggga cgtggccgac cggttcttca cgtcgtaccg
     5161 ggagtgttcc cccgccagac ccggacgtcc cgcgtcccgg ggcttcaggt acaggggcgt
     5221 gcgccgcgcc gggtccgtgt ggaggtgggt ccgatccacg cccagggctg ccaggtcctg
     5281 ccggagctcc cagccctccc cgtcgtcccc ggtgaagccg acggcgacaa ggcggccggt
     5341 ccccagggcg gccaggttgt tcaccaccgt gcccgcggcg cccaggtcaa cgccccagtg
     5401 cggcaggccg ccgta
//
LOCUS       DAOTEI010000212         5366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_11029_length_5366_cov_3.698174, whole genome shotgun sequence.
ACCESSION   DAOTEI010000212 DAOTEI010000000
VERSION     DAOTEI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11029_length_5366_cov_3.698174"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /gene="flgG"
                     /locus_tag="P5313_15175"
     CDS             complement(<1..775)
                     /gene="flgG"
                     /locus_tag="P5313_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRY81755.1"
                     /translation="MVRSLWTAASGMIGQQANIDTISNNLANVNTSGFKKVRADFEDL
                     IYQTVRVAGTPATEDTVVPVPVQMGHGVKLAATQRQFTQGALQNTENLSDIAIQGDGF
                     FRILLYDGTYGYTRDGAFKIDSNGQIVTSNGYRLLPEVTLPENFIPETLTISQDGRVN
                     VKVPGNDDPVLVGQLELYRFPNPVGLTAIGENLFKISNASGDPIAGRPGYDGMGKTIH
                     KFLEMSNVSVVREMVNMIVAQRAYEFNSKAIQTSDNMLGT"
     gene            complement(779..1630)
                     /gene="flgF"
                     /locus_tag="P5313_15180"
     CDS             complement(779..1630)
                     /gene="flgF"
                     /locus_tag="P5313_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRY81756.1"
                     /translation="MIRGWYTGASGMAAQQVRLDAISNNLANVDTDGYKRETAVHKAF
                     AQLLLRRINDDGQYLHPLGSGDMAPVIGKLGTGVETNELFVEFQQGALKQTENDFDLA
                     LDGRGFLCVRTPDGERYTRNGGFILGKEGYLETKDGYPVLGENGPIRIKANNFQVDAQ
                     GKIWVNRSWADDDPTRLVGREQNEWEDTFHMDTLKLVDFKRDRYLAKQGSSLYASTEE
                     SGEARVASAGQGPKVIQGFVEASNVNPVTEMVRMIEVNRAYEANQKTIQSHDGMLGKL
                     INEVVRV"
     gene            2169..2504
                     /locus_tag="P5313_15185"
     CDS             2169..2504
                     /locus_tag="P5313_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81757.1"
                     /translation="MIRYEFSAEASRIVRSEIRELLAWSRRADTISFGGGLPDPSLFP
                     VEDLERICAEVLGRKGYLALQYAPPASGFRPSAQPWNPSRAIWGSTETRPSRARSASS
                     PPPSRPWTS"
     gene            2426..3433
                     /locus_tag="P5313_15190"
     CDS             2426..3433
                     /locus_tag="P5313_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY81758.1"
                     /translation="MGIHGDKAEPGSLCVVSSSQQALDLLTLLFLEPGAPAVVEVPSY
                     VGALQAFGRAGADLRGVPVGPEGMDLDALEDTLHGLDREGKRPRFLYVIPDFQNPSGV
                     TLNLPGRRRLLSMAERRGIPIVEDSPYRELSYGAESLPSLWTLSGGRGVILLKTLSKM
                     LFPGFRLGWIAAEEEVLSKIIGLKQSVDLCSSSFLQLVTAAYFESGLMEGTLARARAL
                     YAPKRDAMVEALGRELPEGSTWTRPEGGVFCWATLPDRFDSREVLKAALGEGVAFVIG
                     RAFHCDGGGRSSMRLNYSFPSLEAIREGASRLGRAIRASEPGRPGIDRAAFPAAVEAG
                     A"
     gene            3436..5076
                     /locus_tag="P5313_15195"
     CDS             3436..5076
                     /locus_tag="P5313_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY81759.1"
                     /translation="MFLSRLDRLSREPLARQLADRILEGIETGALAAGDALPSTRRMA
                     DWVGVSRFTVERAYDELWAQGYVEARSGSYTRVRARPPRRARAEGSGTVRPEPAGIRL
                     KNAPASLRPEDRIGPAAAAALAQRERMRSAYLGSAAVTASPPRSRSGSPSGSRIASAS
                     SAGPVHPDRTVIDFYSFHPDSRLFPSESFKRAAAEALRSDPGSALSYGNPAGHPALRE
                     RIAKRMREHGIDAGPDNVLVTEGALHAVDLAGRLLAAPGRSALCEDPTYPGALAAFGA
                     SGLRPLPVPDDDKGMDPAALRAALDREGRDAAFVYLMPSFRNPTGRLTGQRRREELLS
                     ACLEARVPILEDGFEEELGFLGNAVRPLASMDPGAAVLYVGTLSKSLFPGIRLGWIAA
                     SEEWIRPLTALRAATSLGGNMMAQAAAAAFWDSGAYATHLRRVCRAFSRRLRAAQAAA
                     ARHLPASARLAEAGGGYLLWVRLPGGPGPEAARIESEVMEACRREGVAAAPGSPFYAG
                     TSPGPALRLCIAGLAESEIEEGFRRLGAALVKVFRTES"
BASE COUNT          741 a         1820 c         1821 g          984 t
ORIGIN      
        1 cggtgcccag catgttgtcg ctggtctgga tggccttgga gttgaactcg taggccctct
       61 gggccacgat catgttgacc atctcccgga ccaccgagac gttggacatc tccaggaact
      121 tgtggatggt cttgcccatc ccgtcgtagc ccgggcgtcc ggctatgggg tccccggagg
      181 cgttggagat cttgaagagg ttttccccga tggcggtgag gcctacgggg ttggggaaac
      241 ggtagagctc gagctggccc accaggacgg ggtcgtcgtt gccgggaacc ttgacgttga
      301 cccggccgtc ctgggatatg gtcagggtct cggggatgaa gttctccggc agggtgacct
      361 ccggcaggag gcggtagccg ttggacgtga cgatctggcc gttggagtcg atcttgaagg
      421 ccccgtcccg ggtgtagccg taggttccgt catacaggag gatccggaag aagccgtccc
      481 cctggatggc gatgtccgag aggttctcgg tgttctgcag ggccccctgg gtgaactggc
      541 gctgggtggc ggccagcttc acgccgtggc ccatctgcac gggtacgggc accacggtgt
      601 cctcggtggc gggggttccg gcaacccgga cggtctggta gatgaggtcc tcgaagtccg
      661 cccggacctt cttgaacccc gaggtgttca cgttggccag gttgttcgag atcgtgtcga
      721 tgttggcctg ctgtccgatc atgccgctgg cggcggtcca caagcttcgt accatgcgtt
      781 ataccctcac cacttcattg atcaacttgc cgagcatccc gtcgtgggac tggatggtct
      841 tctggttggc ctcgtaggct cggttgacct cgatcatccg caccatctcg gtcacggggt
      901 tgacgttgga ggcctccacg aagccctgga tcaccttggg cccctggccg gcggaggcga
      961 cgcgcgcctc cccgctctcc tccgtggagg cgtagaggct ggagccctgc ttggccaggt
     1021 agcggtcccg cttgaagtcc acgagcttca gggtgtccat gtggaaggtg tcctcccact
     1081 cgttctgctc ccgccccacc aggcgcgtgg gatcgtcgtc cgcccaggag cggttgaccc
     1141 agatcttgcc ctgggcgtcc acctggaagt tgttggcctt gatccggatg ggcccgttct
     1201 cacccaggac gggatagccg tccttggtct ccaggtagcc ttccttgccc aggatgaagc
     1261 ccccgttgcg ggtgtagcgc tcgccgtccg gggtccggac gcagaggaag cccctgccgt
     1321 ccagggccag gtcgaagtcg ttctccgtct gcttgagggc gccctgctgg aactcgacga
     1381 acagctcgtt cgtctcgacc cccgtgccga gcttgccgat gaccggcgcc atgtcgcccg
     1441 agcccagggg gtggaggtac tggccgtcgt cgttgatccg ccgcaggagg agctgggcga
     1501 aggccttgtg cacggccgtc tcccgcttgt agccgtcggt gtccacgttg gccaggttgt
     1561 tggagatcgc gtccagccgc acctgctggg ccgccatgcc gctggcgccg gtgtaccatc
     1621 ctcgaatcat ccggagggcc tcctacgata gaagcatcgg cagattcggg agggcggttg
     1681 agggggaagc gggggttccc gccggaggga tcgggaggaa tccggggagg cgcaccggat
     1741 ccgggccggc gcactatctc ctgcttcata taccatacgg gtttgttcaa ttatgcgaac
     1801 ttcagataac tcgccccatc gtcgcattat acgaagatca cagaacttgc cctctcgtcg
     1861 tgttatacga acttcatata actcgctccg taaatgagtt atctgaagct cccggcgaat
     1921 ccccggcgaa tcccctgcga atcccctgcg aatcccctgc ccgggcaggg ctccggatcg
     1981 tcccgaggac ccggcgcgcc tctttgtgcc ggtgcctgtc gatcgtcggg tatctcggcc
     2041 cctaatcggc ctggcccatt atttaactat tggctatgta caccatgccg attcgggtgc
     2101 atcctgaccc tgccggggaa ggcggaccgg tccggccggc gccggcctcc cgcggcgggg
     2161 aggaaagaat gatccgatac gagttttccg cggaggcgtc gcgcatcgtg cggtccgaaa
     2221 tccgggagct cctggcctgg tcccgcaggg cggacaccat ctccttcggg ggcggcctgc
     2281 cggatccctc gctcttcccg gtggaggacc tcgagcggat ctgcgccgag gtcctgggcc
     2341 ggaagggcta tctggccctc cagtacgcgc cgcccgcatc cggcttccgg ccctccgctc
     2401 agccctggaa tccttctcgc gctatatggg gatccacgga gacaaggccg agccgggctc
     2461 gctctgcgtc gtctcctcct cccagcaggc cctggacctc ctgaccctcc tcttcctgga
     2521 gcccggcgcc ccggcggtgg tggaggtccc ctcctacgtg ggggccctcc aggccttcgg
     2581 ccgggcgggc gcggacctgc ggggcgtccc ggtgggcccc gaaggaatgg acctggacgc
     2641 cctggaggac acgctgcacg gcttggaccg ggaaggcaag cggccccgct tcctctacgt
     2701 catcccggac ttccagaacc ccagcggggt caccctgaac ctgccgggcc ggcggcgtct
     2761 gctgtccatg gccgagaggc ggggtatccc gatcgtcgag gactcgccgt accgggagct
     2821 ctcctacggc gccgagagcc tcccgagcct gtggaccctc tcgggaggcc ggggggtcat
     2881 cctcctcaag accctgtcca agatgctctt cccgggattc cgcctgggct ggatcgccgc
     2941 ggaggaggag gtcctgtcca agatcatcgg cctcaagcag tccgtggacc tctgctcctc
     3001 ctccttcctc cagctggtca ccgcggccta cttcgagtcg ggcctcatgg agggcaccct
     3061 ggcccgggcc cgagccctgt acgcccccaa gcgggacgcc atggtcgaag ccctgggccg
     3121 ggagctcccg gagggctcga cctggacccg acccgagggt ggggtcttct gctgggcgac
     3181 tctgccggac cggttcgact cccgggaggt gctgaaggcg gccctgggcg agggggtggc
     3241 cttcgtgatc ggccgtgcct tccactgcga cggcgggggg aggtcctcga tgcgcctcaa
     3301 ctactccttc ccctccctcg aggcgatccg ggagggggcg tcccggctgg gccgggcgat
     3361 ccgggcttcg gagccgggcc ggcccgggat cgatcgcgcc gcgttcccgg ccgccgtcga
     3421 ggcgggagcc taaccatgtt cctgtcccgc ctggaccggt tgtcccggga gcccctggcc
     3481 cgacagttgg cggatcggat cctggagggc atcgaaaccg gggccctggc cgcgggggac
     3541 gccctgccct cgacccggcg catggcggac tgggtcgggg tctcccggtt cacggtggag
     3601 cgggcctacg acgagctctg ggcccagggc tacgtggagg cccggagcgg ctcctacacc
     3661 cgtgtccgcg cccggcctcc ccgcagagcc cgggcggaag ggtccgggac ggtgcgaccg
     3721 gaacccgccg ggatccgtct taaaaacgcc cctgcatccc tccgccccga agaccggatc
     3781 ggtcccgccg cggcggccgc cctggcccag agggagcgga tgcggtccgc gtacctgggt
     3841 tcggcggccg tcaccgcgtc gccgccccgg tcccgttcag gctcgccctc gggatcccgc
     3901 atcgcctccg cctcgtccgc tgggcctgtc catccggatc ggacggtcat cgatttttat
     3961 tccttccacc cggactcccg gctctttccc tcggagtcct tcaagcgggc agcggcggag
     4021 gccctgcgtt ccgatccggg atccgccctg tcctacggga atcccgcggg gcatcccgcc
     4081 ctccgggaac ggatcgccaa gcggatgcgg gagcacggga tcgatgcggg tccggacaac
     4141 gtcctggtga ccgagggggc cctccacgcg gtggacctgg ccggccgcct cctggcggcc
     4201 ccgggacgct cggccctctg cgaggacccg acctacccgg gggccctggc cgccttcggg
     4261 gcctcgggtc tccggcccct gccggtgccg gacgacgata aggggatgga tcccgccgcc
     4321 ctccgcgccg ctcttgaccg ggaaggccgg gacgcagcct tcgtctacct catgccctcc
     4381 ttccggaacc ccacgggcag actcacgggc cagcgccgac gggaggaact gctctcggcc
     4441 tgcctggagg cgagggtgcc gatcctggag gacggcttcg aggaggagct gggcttcctg
     4501 ggaaacgccg tgcggcccct ggcctccatg gatcccggcg ccgcggtcct ctacgtgggg
     4561 accctgtcaa agtccctctt cccggggatc cgcctgggct ggatcgccgc ctcggaggag
     4621 tggatccggc ccctgaccgc cctgcgggcc gcgaccagcc tgggggggaa catgatggcc
     4681 caggcggcag ccgcggcttt ctgggactcc ggggcctacg ccacccacct gcgccgggta
     4741 tgccgggctt tctcccgccg tctccgggcc gcccaggcgg ccgcggctcg gcacctgccg
     4801 gcgagtgccc ggctcgcgga agccggagga ggctacctcc tctgggtgcg cctcccggga
     4861 ggccccgggc cggaagccgc ccggatcgag tccgaggtga tggaggcctg ccgccgggag
     4921 ggggtggccg cggccccggg ctcgcctttc tacgcgggga cttcaccggg ccccgccctg
     4981 cggctgtgca tcgccggtct cgcggagtcc gagatcgagg agggcttccg ccgcctcggc
     5041 gccgcgttgg taaaggtttt ccggacagag agttaacaag atggacaaga tttggataga
     5101 tgaacaagat ggagctatgg gatcaggaga gttctgatca ttgaaaagga ttgttcctgc
     5161 ttattcctat tctctacttc ttcattatct tgttcatctc gtcttcatcc tgtccatcct
     5221 gttggacctg tccggaaacc tcacgtggtg gtcagcttcc cagcatctat ctcattgagg
     5281 aaaatcctgt cgtaccggcc cgagtgggtg gccgtaggga ggccgggggc cagggtttcc
     5341 tggaagaaaa aaaggacgtc ctccat
//
LOCUS       DAOTEI010000213         5314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_11181_length_5314_cov_4.584141, whole genome shotgun sequence.
ACCESSION   DAOTEI010000213 DAOTEI010000000
VERSION     DAOTEI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11181_length_5314_cov_4.584141"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="P5313_15200"
     CDS             <1..311
                     /locus_tag="P5313_15200"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HRY81760.1"
                     /translation="SRPQKLVLAAGLSDRTVELDSSVPGDVPQAHRLTLVDLESEEPL
                     SISADPLCRFLQAPMYTPVDAGEGAEPLYQGCAVTLEWPVSLRPDASWKASITLGVRE
                     "
     gene            357..1817
                     /gene="murC"
                     /locus_tag="P5313_15205"
     CDS             357..1817
                     /gene="murC"
                     /locus_tag="P5313_15205"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRY81761.1"
                     /translation="MAGSLVPAQIAGRSIHMVGIKGTGMAALAEILSAGGARLSGSDV
                     SEVFYTDAILAALGIEVFPFDRGNVRAGIDLVIHSAAYVRGVHPELAAAEDLGIPILS
                     YTEALGELSRRFDSSGIAGVHGKTTTTALTGTLLRGAGSSSTILAGSAVAGFGDRSTL
                     ILGKRFFVAETCEYKRHFLSFSPARIVLTSVEPDHQDYYPDYDSIRDAFLEYVLSLPP
                     RGCLIYCVDDPGASDVAGRALALRPDLDAVPYGWTAEGRWKLESYRIGNERAEFRLSG
                     FPEPFRLRVPGRHVALDAAAALALTERLVREEFGRPLEGTRLESVRAALESFSGSRRR
                     CEVLGLARGILFMDDYGHHPTAIRLTLEGIREFYPDRRLVVDFMSHTYSRTAALLDGY
                     ADAFSAADEVILHKIYASARERPDGTVTGRTLFERMSARRPRVRYFEEPLDARDALRA
                     ELGPGDLFLTLGAGDNFRLGRALYESIKAEEEPSTP"
     gene            1814..2677
                     /locus_tag="P5313_15210"
     CDS             1814..2677
                     /locus_tag="P5313_15210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRY81762.1"
                     /translation="MKSMTGFARREIETPFLKGSLTIKSYNNRYLEISVYLPPYLAAL
                     ESRFRDAIAASVIHGKVEASLRVREVSEPPRVLVDAAAASEVASALRTAAEAAGIREP
                     LRLSDILSFDGVVTFERNIDADTAWTALEEPVSGCLAEFDRDREREGRSTRADIESKL
                     AVIEESVGVVEGEAPAIEAAIRGNLASRFREVLGSEADENRVLQEVASYLARHTINEE
                     IVRLRSHVAAFRAALEDPGCGKKLDFLCQEMNREANTIGSKNILARIGEAVVSLKDAI
                     ENVREQVRNVE"
     gene            2692..3234
                     /locus_tag="P5313_15215"
     CDS             2692..3234
                     /locus_tag="P5313_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HRY81763.1"
                     /translation="MERSPFIIAVSGKSGCGNTTVSGLLRERLGLRLINFTFRSLAAE
                     RGITLQKVLELARDDPAWDREVDQRQVAMAREGDCVIGSRLAIWMIPEAALSVYLHAS
                     PETRARRIWNREGGKYEEVLAFTQSRDAQDTARYAELYGIDNNRYDFADLSINTERLG
                     PDRIVDIIATAVKGLRDVCP"
     gene            3267..4082
                     /locus_tag="P5313_15220"
     CDS             3267..4082
                     /locus_tag="P5313_15220"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY81764.1"
                     /translation="MTTQGSGDVVLVTGATSGLGRACAERLAARAYRVYGTGRKPSAP
                     EAGGVILLSMDVTDDQSVRKAVREILDREGRIDAVVCNAGYGIAGPVEETPLAEAKAQ
                     FETNFFGTLRTIQAVLPAMRAAGRGRVLVVGSMAGLAGVPYQSLYSAAKFALEGLVEA
                     LRMETRAFGIRCAIVEPGDTRTGFTDARKKSAAPGSPYAAAMERSLGVMEKEERNGSD
                     PAVMARLVERLLRKDRLRVRYMVGPGFQKLAMTLKRVLPSRTYEWIMASYYRV"
     gene            complement(4101..>5314)
                     /locus_tag="P5313_15225"
     CDS             complement(4101..>5314)
                     /locus_tag="P5313_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81765.1"
                     /translation="DGGIGAEHGISGRGIRGVLLTWEEASDPTAGYSVFRSDSPIDRG
                     SFPAAERLKDLPPGSLQYLDVPPDSRGYYYAVLGRTEQGGVYEVFIPLKNVSIAPVTY
                     VPPAAPVPEPDPKPVEPAPEPPPAVRASSERDGIRVVWEPVRPGRRVVLYRSPSPIRS
                     LSDLLTASWVASAEESPEGILDFPVPGIGYFYALVDDEGLRTGSPELAVGLNVTAESV
                     SVPAGRFRIGLPEIPYASRSLPLPYLSLTRGVEAGSQSLPGNLPVPSPSASPLRPETQ
                     KSVSRLGGSGKNLPSSPPELRILPEDLELSAVGGEEYTLRTIVRDRLARKDWKGASDE
                     LARYLSLNRSPSAAARARFYRGQALAYSGQYREAFFEFLLAQDDLYAPSNLWIDWLLD
                     QMKASGPAVGR"
BASE COUNT          902 a         1737 c         1829 g          846 t
ORIGIN      
        1 cctcccggcc ccagaaactg gtgctggccg cggggctctc ggacaggacc gtggagctgg
       61 actcctccgt acccggggat gtcccccagg cccaccgcct gacccttgtg gacttggaat
      121 ccgaggaacc cctgtcgatc tcagcggatc ctctgtgcag gttcctccag gccccgatgt
      181 acactccggt cgacgccggg gagggtgcgg agcctttgta ccagggctgc gccgttaccc
      241 tggaatggcc cgtatccctc cgccccgacg catcctggaa agcatccata accctcggcg
      301 tccgggaatg aacggctctc gttgcaatga accggcctga tcgctatcat gcccccatgg
      361 caggatccct agtgcccgcc cagatcgcgg gacgttccat ccacatggtc ggcatcaagg
      421 gcaccggaat ggcggccctg gcggaaatcc tctccgccgg aggggcccgc ctctcgggca
      481 gcgacgtatc cgaggtcttc tacaccgacg cgatcctggc ggccctgggc atcgaagttt
      541 tccccttcga ccggggtaac gtgcgggcgg ggatcgatct ggtcatccac tccgccgcct
      601 acgtccgggg agtccacccg gaacttgccg cggccgagga cttgggcatc ccgattctga
      661 gctacacgga agccctgggg gagctgtccc ggagattcga ctcctccgga atcgccgggg
      721 tccacggcaa gaccacgacg acggccttga cgggtaccct cctgcgggga gcgggttcct
      781 cctcgacgat cctggcgggg agcgccgtgg cgggtttcgg cgaccggtcg accctgatcc
      841 ttgggaagcg gttcttcgtg gcggaaacct gcgaatacaa gcgccacttc ctgtccttct
      901 ctcccgcccg gatcgtcctc acctccgtgg aaccggatca tcaggattac tatcccgact
      961 acgattccat ccgggacgcg tttttggaat atgtcctctc cctgccgccc cggggatgcc
     1021 tgatctactg cgtcgacgat cccggggcct cggacgtcgc aggccgcgcc ttggcccttc
     1081 gcccggacct ggacgcggtg ccctacggat ggacggccga gggacggtgg aaactggaat
     1141 cctacagaat cggaaacgag cgggcggagt ttcgactctc ggggttcccg gaacccttcc
     1201 ggttgcgcgt cccgggccgg cacgtggccc tggacgcagc ggcggccctg gccctgacgg
     1261 agcggctggt gcgggaggag ttcggacgcc ccctggaggg aacgcggctg gagtcggtcc
     1321 gggccgccct ggagtctttt tccggatccc ggcgccgctg cgaggtcctg ggcctggccc
     1381 ggggcatcct gttcatggac gactacggcc accatcccac cgcaatccgg ctgaccctgg
     1441 aaggcatccg ggaattctat ccggaccgga gattggtggt ggatttcatg tcccatacct
     1501 attcccggac cgccgccctc ctggacggct acgcggacgc cttttccgcg gccgacgaag
     1561 tgatcctgca caagatctac gcctccgccc gtgagcggcc ggacgggacg gtgacaggac
     1621 ggaccctgtt cgagaggatg tccgcccgcc gccctcgggt ccggtacttc gaggaacccc
     1681 tggacgctcg ggatgccctt cgtgcggagc tcgggcccgg ggatctcttc ctgaccctgg
     1741 gcgccgggga caacttccgc ctgggaagag ccctctacga atccatcaag gccgaggagg
     1801 aaccttcgac accatgaaga gcatgaccgg attcgcgcgc cgggaaatcg agaccccctt
     1861 cctgaagggt tccctcacga tcaagtccta caacaaccgc tacctcgaga tctccgtgta
     1921 cctgcctccg tacctggcgg ccctggaatc ccggttccgg gacgcgatag ccgcatcggt
     1981 catccacggc aaggtggagg cgtcccttcg ggtccgggag gtctccgagc ccccccgggt
     2041 cctggtggac gccgcggcgg cttcggaagt cgcgtccgcc ctgcggaccg ccgccgaggc
     2101 cgcggggatc cgggaacccc tccggctctc cgacatcctc tccttcgacg gggtcgtcac
     2161 cttcgagcgg aacatcgacg cggacacggc ctggacggcc ctggaggaac ccgtctccgg
     2221 gtgcctggcc gaattcgacc gggatcggga acgggaaggg cggagcaccc gggcggatat
     2281 agagagcaag ctcgccgtca tcgaggagtc cgtgggagtc gtggaagggg aggcgccggc
     2341 catagaggcc gctatccggg gaaacctcgc ctcgcggttc cgggaggtcc tgggcagcga
     2401 agcggacgag aaccgggtcc tgcaggaggt ggcttcctac ctggcccggc ataccatcaa
     2461 cgaggagatc gtccgtctgc ggtcccatgt cgcggcgttc cgcgcggccc tggaggaccc
     2521 gggctgcggg aagaagctgg atttcctctg ccaggagatg aaccgggagg cgaacaccat
     2581 cggctccaag aacatcctgg cccggatcgg ggaggccgtc gtctccctga aggatgccat
     2641 cgagaacgtc cgggaacagg tccggaacgt ggaatgaacc cgggaggagc catggaacga
     2701 tcgcccttta tcatcgccgt gtccggcaag agcggctgcg ggaacaccac ggtgagcggc
     2761 ctgctgaggg agcgcctggg actccggctc atcaacttca ccttccgcag cctggccgcg
     2821 gagaggggga taaccctcca gaaggtgctg gagctggccc gggacgaccc ggcctgggac
     2881 cgggaggtgg accagcgcca ggtggccatg gcccgggagg gggactgcgt catagggtcc
     2941 cgcttagcca tctggatgat cccggaagcc gctctgtcgg tctacctcca tgcctccccg
     3001 gagacccggg cccgccggat ctggaaccgg gaaggcggga agtacgagga ggtcctggcc
     3061 ttcacccaat cccgggacgc ccaggacacg gcccggtacg cggagctcta cgggatcgac
     3121 aacaaccgct acgatttcgc ggacctgtcc ataaacaccg aacggctggg accggatcgg
     3181 atcgtggaca tcatcgccac cgccgtgaag ggtctacggg acgtctgccc ctgaacggga
     3241 tcaaagccaa gacaggaagg tacattatga caacacaggg atccggagac gtggtcctcg
     3301 tgacgggagc gaccagcggg ctcggcaggg cctgtgccga acgcctggcg gcccgtgcgt
     3361 accgtgtcta cgggacgggg aggaaaccgt cggcgccgga agccgggggg gtgatcctgc
     3421 tgtccatgga cgtcaccgac gaccagtccg tgaggaaggc tgtccgggaa atactggacc
     3481 gggagggccg gatcgatgcg gtggtctgca acgccggcta cggcatcgcg ggccccgtcg
     3541 aggagacgcc cctggcggag gccaaggccc agttcgagac gaacttcttc ggcaccctca
     3601 ggaccatcca ggccgtgctg cccgcgatgc gggcagcggg ccggggccgg gtgctggtgg
     3661 tgggttccat ggcgggcctg gcgggggttc cctatcaatc cctgtactcc gccgcgaagt
     3721 tcgccctgga aggcctggtc gaggccctcc ggatggaaac ccgggccttc ggaatacgct
     3781 gcgccatcgt ggagcccggg gataccagga cggggttcac ggacgcccgg aaaaagtccg
     3841 cggctccggg aagcccctac gccgccgcga tggaacgttc cctgggggtt atggagaagg
     3901 aggagcggaa cggctccgat cccgcggtca tggctcgcct cgtggaacgg ctcctgcgga
     3961 aggaccggct gcgggtccgg tacatggtcg gcccgggatt ccagaaactg gccatgacct
     4021 tgaagcgggt cctgccttcc cgtacctacg agtggatcat ggcctcgtac tacagagtct
     4081 gagcgagccc gcggacccac ctaccgtcct acggccgggc cggacgcctt catctggtcc
     4141 aacagccaat cgatccagag gttggaaggc gcgtacaggt cgtcctgggc caggaggaat
     4201 tcgaagaagg cctcccgata ctgcccggaa taggccaggg cctggccgcg gtagaaccgg
     4261 gcccgggccg cggcggaggg gctgcggttc agggaaaggt accgggcgag ctcgtccgag
     4321 gcccctttcc agtccttccg ggccagtcga tcccggacga tcgtgcgcag ggtgtattcc
     4381 tcgccgccga cggcggacaa ctccagatcc tcgggaagga tgcggagttc gggaggggac
     4441 gagggaaggt tctttccgct cccgccgagc cgggatacgg acttctgggt ctccggcctc
     4501 aacggagagg cggagggtga aggcacgggc aagttcccgg gcaaggactg ggatcccgcc
     4561 tcgactcccc gggtcaggga gaggtagggg aggggcaggg accgggacgc gtagggaatt
     4621 tcgggcagcc cgatacggaa gcggcccgcg ggaaccgaca cgctttccgc cgtgacattc
     4681 agccccaccg cgagctccgg gcttccggtc cgcagtccct cgtcgtccac cagggcgtag
     4741 aagtatccga ttccggggac cgggaaatcc aggatgccct ccggggattc ctcggcgctg
     4801 gccacccagg aggccgtcag caggtccgaa agggaccgga tgggggacgg cgaccggtac
     4861 agcaccaccc gtctgcccgg acgcacgggt tcccagacga cacggatgcc gtcccgttcg
     4921 gaggaggcgc gcaccgcggg agggggttcc ggcgcgggtt cgacgggctt gggatccggt
     4981 tccggcaccg gggcggcggg aggcacgtag gtgaccgggg ctatgctgac gttcttgagg
     5041 ggtatgaaaa cctcgtatac cccgccctgc tcggttcggc ctaaaaccgc gtaataatat
     5101 ccccgggaat ccgggggtac gtccaggtat tggaggcttc ccgggggcag gtccttcaat
     5161 cgctccgccg ccgggaagga gcctcggtcg atgggggaat cggagcggaa cacggaatat
     5221 ccggccgtgg gatccgaggc ttcctcccag gtgaggagga cgcctcggat cccacggccg
     5281 gatattccgt gttccgctcc gattccccca tcga
//
LOCUS       DAOTEI010000214         5274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_11310_length_5274_cov_4.470013, whole genome shotgun sequence.
ACCESSION   DAOTEI010000214 DAOTEI010000000
VERSION     DAOTEI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11310_length_5274_cov_4.470013"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="P5313_15230"
     CDS             complement(<1..346)
                     /locus_tag="P5313_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81766.1"
                     /translation="MRCARKPGVPGGQVLAGILIAGTALLGSSCSGHAPELVSLEYRL
                     NLRPADPRGASASEQLSIFAAVRDEDGVKDLAALHVLHDGRETYWTLTPETWTRNDRG
                     KETWVGGTGLSPV"
     gene            complement(349..1656)
                     /gene="hisS"
                     /locus_tag="P5313_15235"
     CDS             complement(349..1656)
                     /gene="hisS"
                     /locus_tag="P5313_15235"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRY81767.1"
                     /translation="MSDIIQPRVLKGFRDFLPDAEMERRDLTERLEKVFRTFGFVPID
                     TPALEYAEILLGKGGGETDKQVYRFRDHGDRDVALRFDLTVPFARFMAEHAGELYLPF
                     KRYHIAKVWRGENTQRGRYREFTQCDFDVVGADCASSDFETIQVIADSMRALGVEDFR
                     IRINHRGVFNRFLASAGAESASSDILRIVDKLEKAGTEEVRAQLAAAAGEGTADRVLD
                     FTRKEKDFPETLAKITAAAGGDNPDSARLGEVFECARAAGISDRLVLDPSITRGLDYY
                     TGVVFETSLEALPEIGSVCSGGRYNELASLYTRQNLPGVGAAIGLDRLLAALEALGRS
                     NRRSGNTRVLIFHVEDGFLPRYWELARELRAAGIPCEVYPENRKLSQQFAYAERKSVP
                     FGIFLGAAESARGVLAVKNLATRESREYPSVREAAADLAGGSA"
     gene            1867..2622
                     /locus_tag="P5313_15240"
     CDS             1867..2622
                     /locus_tag="P5313_15240"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRY81768.1"
                     /translation="MAIDVGNTNIVGGVYEGNVLRETLRIQTVTKKTEDEYVTVFRAI
                     LTERDIDPASIDRVIVSSVVPALTGSISAMVVRLFRTEPIVLGPALYSRLPVKVIAQD
                     EIGTDLVADAVAAYGKVGGACIVVDFGTALTFTCVSGAGEIRGVSIAPGLGTAVGALS
                     RDTAQLPNVPLAPPPSVLGQNTVQAIQAGVVYGYTGLVEYLIGRMVRELGAEAKIVAT
                     GGLCRVVAPLTDVFDLVEPDLTLDGLARIARYV"
     gene            complement(2626..3081)
                     /locus_tag="P5313_15245"
     CDS             complement(2626..3081)
                     /locus_tag="P5313_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81769.1"
                     /translation="MAQQKIRIQDDLWYVRRLVSLVSDAVKVQPDADLFATKVLEDAV
                     FAQRALKEFKELLESNPRLMDRAEYLVLLSRSARSLAEAVSDLASGEGELSRGAATRG
                     TDLEILTRDLRSLAAEIRDLASAALNDGTSDEDVVSGDELSELLRGEAP"
     gene            3231..3512
                     /gene="rpsF"
                     /locus_tag="P5313_15250"
     CDS             3231..3512
                     /gene="rpsF"
                     /locus_tag="P5313_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRY81770.1"
                     /translation="MRSYELVSIFPTEEELFRVGKESVAAELVKQGAQNIKEEDMGDR
                     PLAYPIRKKVRGHYILFSMQFPGENVDATEKVFKLNPNILRYLFVRADA"
     gene            3534..4013
                     /gene="ssb"
                     /locus_tag="P5313_15255"
     CDS             3534..4013
                     /gene="ssb"
                     /locus_tag="P5313_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRY81771.1"
                     /translation="MSSFSVVVLVGNLTRDAQLKYTPSGFPICTFGIAINTRKKNGDQ
                     WVDEANFFDIELFGKSAESLNQYLTKGKMVGVEGELRQDRWEQDGQSRSKVKIVANKV
                     NLLGGGNREGSYSGGGAPAQGGSGWRDQAERPAPKGRPAPGESTPPETDDFSDDIPF"
     gene            4100..4438
                     /gene="rpsR"
                     /locus_tag="P5313_15260"
     CDS             4100..4438
                     /gene="rpsR"
                     /locus_tag="P5313_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRY81772.1"
                     /translation="MRPPRDDQDSRRDGPRDGGREGPRDGGRDGEDGGRGGPRGGRKG
                     FFRKKVCKFCTNKLRIDYKDPDTLRRFVTERGKILPRRITGSCAKHQRALAVAIKRAR
                     ALAFLPFVSK"
     gene            4453..>5274
                     /locus_tag="P5313_15265"
     CDS             4453..>5274
                     /locus_tag="P5313_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81773.1"
                     /translation="METKSARIPEFRYLALGAVAAFLYGSTVFSLFFAMPLQTSWSRG
                     GYRAFFVSLSVALAGTAGWLFWQYRGLPFGDLGGFLLMLSLPAGLAGAMALANAGFLA
                     RLPLVYRVMAGSLVIVAISLPSIYSLFASPNVQAYLLQGIEEMTRTMDSVGGEGYAGA
                     VLRSSLEPEAMLQAARRVIADGFAAVLFGATFLGHWLGTRAAGADAPGSRKVPALSSF
                     SVPGQLIWAFLGSWSAVLSLRLPYLAGLRSAPFLEALAWNAALTVSLCFAVQGLGI"
BASE COUNT          880 a         1779 c         1705 g          910 t
ORIGIN      
        1 caaccgggga gagtccggtc cccccgaccc aggtctcctt gccccgatcg ttccgggtcc
       61 aggtctcagg ggtcagggtc cagtaggtct cccggccgtc gtgcaggacg tgcagggcgg
      121 ccaggtcctt gaccccgtct tcgtcccgga ccgccgcgaa gatcgagagc tgctccgacg
      181 ccgaggcgcc ccggggatcc gcgggccgaa ggttcaggcg gtattccagg gagaccagtt
      241 cgggggcatg gccgctgcag gacgaaccca ggagggcggt tcccgcgatc aggattccgg
      301 cgaggacttg cccgcccggt acccccggtt ttcgggcgca ccgcacggct aggccgatcc
      361 tcccgccaga tccgccgcgg cttcccgaac ggacgggtac tcccgggatt cccgggtagc
      421 cagattcttg accgcgagga cgccccgggc gctttccgcg gccccgagga atatgccgaa
      481 gggaacggat tttctctccg cgtaggcgaa ctgctgggaa agcttgcggt tctccggata
      541 gacctcgcag gggattccgg cggcccggag ttcccgggcc agttcccagt atcggggtaa
      601 aaatccgtcc tcaacgtgga agatcagcac ccgggtattc ccggaccgcc ggttcgagcg
      661 gcccagggcc tccagggccg caaggagtcg atccaggccg atggccgccc cgactcccgg
      721 aaggttctgc cgggtgtaca gggacgccag ctcgttgtac cgccctccgg agcaaaccga
      781 gcctatctcg ggcagggcct ccagggaggt ctcgaacacc acgccggtgt agtaatccag
      841 gccccgggtt atcgagggat ccaggaccag tctgtccgag atccccgcgg cccgggcgca
      901 ctcgaaaacc tccccgagac gggcggagtc gggattgtcc ccccccgcgg cggcggtgat
      961 cttggccagg gtctccggaa aatctttttc tttccgggtg aagtcgagga cccggtccgc
     1021 ggtcccttcc cccgccgcgg ctgccagctg ggcccgcacc tcttcggttc ccgccttctc
     1081 gagcttgtcc acgatccgga ggatgtccga ggaggccgac tcggctcccg ccgacgcgag
     1141 gaaccggttg aaaactcccc ggtggttgat ccggatgcgg aaatcctcca ccccaagggc
     1201 ccgcatggag tcggcgatca cctggatggt ctcgaaatcc gaggaggcgc agtccgcccc
     1261 caccacgtcg aaatcgcact gggtgaattc ccgatatcgg ccgcgctggg tgttctcgcc
     1321 gcgccacacc ttggcgatgt ggtaccgctt gaagggcagg tagagctccc cggcatgctc
     1381 cgccatgaag cgcgcgaagg ggaccgtgag gtcgaagcgc agggcgacat cccgatcgcc
     1441 gtgatcccgg aatcggtaca cctgtttgtc cgtctcgcct ccgccctttc ctaaaaggat
     1501 ttcggcgtac tccagggccg gcgtgtcgat ggggacgaag ccgaaggtcc ggaaaacctt
     1561 ctccagccgc tccgtaaggt cccggcgctc catctccgcg tcgggcagga aatcccggaa
     1621 tcccttgagc acccggggct gtatgatgtc gctcatgggg ggagtatggc ggaaaatggg
     1681 ggagggtgta caggagtgtg cggtaaacgg tgaaccgcgg atggtataat ctcagggacc
     1741 ggaaaaatgc gccagatccg tagcaggcgt ccgcgggtac ccggacacat tcccgttcac
     1801 ggaccaccgt tcaccaaccc ccgccttgac atcccctcgt ccctggccga tgatcccctc
     1861 atgctgatgg ccatcgacgt tggaaacacg aatatcgtgg gcggcgtgta cgaggggaac
     1921 gtgctccggg agaccctccg gatccagacg gtgaccaaga agaccgagga cgaatacgtc
     1981 acggtcttcc gggccatttt gaccgagcgg gacatcgacc cggcctcgat cgaccgggtg
     2041 atcgtgagct ccgtggtacc cgccctcacg ggctccatat ccgccatggt cgtgcggctc
     2101 ttccggaccg aaccgatcgt cctgggaccg gccctctatt cccggctccc cgtgaaagtg
     2161 atcgcccagg acgagatcgg gacggacctg gtggccgacg cggtcgcggc ctacggcaag
     2221 gtcggaggag cctgcatcgt cgtcgacttc ggcacggccc tgacttttac ctgcgtgtcc
     2281 ggcgccgggg agatacgggg ggtttccatc gcccccgggc tgggaaccgc cgtgggcgcc
     2341 ctgtccaggg atacggccca gctccccaac gtgcccctgg cgcccccgcc ctcggtgctg
     2401 ggccagaata ccgtccaggc catccaggcc ggcgtggtgt acggctacac ggggctcgtg
     2461 gagtacttga tcggccggat ggtgcgggaa ctgggggccg aggccaagat cgtcgccacg
     2521 ggcggacttt gccgggtggt cgcgcccctg accgatgtat tcgacctcgt ggagccggac
     2581 ctcaccctgg acggactcgc gcggatcgcc cggtacgtct agggatcagg gagcctcgcc
     2641 ccgcagcagt tccgaaagtt cgtcgccgga taccacatcc tcgtccgagg tcccgtcgtt
     2701 cagggcggcg ctggccaggt cgcggatctc cgcggccagg gatcgcaggt cccgggtgag
     2761 gatttccagg tccgtgccgc gggtcgccgc cccccgggaa agctcgccct ccccggaggc
     2821 caggtcggag acggcctctg caagagaccg ggcgctccgg gacagcagga ccaggtattc
     2881 ggcgcggtcc atgagccggg gattggactc caggagctcc ttgaactcct tcaaggcgcg
     2941 ctgggcgaag accgcgtcct ccaggacctt cgtggcgaac aagtccgcgt cgggctggac
     3001 cttgacggcg tcggagacca aggagacgag ccggcggacg taccacaggt cgtcctggat
     3061 tctgattttc tgctgggcca tgatcccccc gttccagcat cggcgccttt caggccctag
     3121 tcaagcccgg gggagctgtc caccttgatg tttccccccg ttcctgatat tgtaccttgc
     3181 ctctttatcc gtcccttgga ggacggatcg aaagtccata gggaggaact atgcgatcct
     3241 acgaactcgt ctcgatcttc ccgaccgagg aagaactctt ccgtgtcggg aaggaatccg
     3301 tggcggccga gcttgtcaag cagggcgccc agaacatcaa ggaagaggac atgggcgacc
     3361 ggcccctggc gtaccccatc cggaagaagg tccgggggca ctacatcctc ttttccatgc
     3421 aattccccgg tgaaaacgtc gacgccacgg agaaggtctt caagctcaat cccaacattc
     3481 tccggtacct cttcgtccgg gcggacgcct aacaggcgga agggcggcga aacatgtcca
     3541 gtttcagcgt tgtcgtattg gtgggaaacc tgactcgcga cgcccagctc aagtataccc
     3601 cctcgggatt cccgatctgc accttcggaa tcgcgatcaa tacccggaag aagaacgggg
     3661 accaatgggt ggacgaagcc aacttcttcg acatcgagct cttcgggaag agcgccgaat
     3721 ccctcaacca gtaccttacg aaaggcaaga tggtgggggt ggaaggcgag ctgcgccagg
     3781 accgatggga gcaggacggc cagtcccgga gcaaggtcaa gatcgtggcc aacaaggtga
     3841 acctcctggg aggagggaac cgtgagggtt cctactcggg aggcggagcc cccgcgcagg
     3901 gcggttccgg ctggcgcgac caggccgagc ggcccgcccc gaaaggccgg cccgctccgg
     3961 gcgaatcgac tcctccggaa accgatgact tttccgacga catcccgttc tgaaaaccga
     4021 acgaacattc aaggagacca gtatgtccga tattcgagag aaccaggaat cccccgcggc
     4081 ccgggagccc cgggaggaaa tgcgccctcc ccgggatgat caggattccc gccgggacgg
     4141 cccccgagac ggcggccgcg aaggcccccg ggacggcggc cgggacggcg aggacggcgg
     4201 ccgcggcggc ccccggggag gccgcaaggg cttcttccgg aagaaggtct gcaagttctg
     4261 caccaacaag ctccgcatcg actacaagga tccggacacc ctccgccgtt tcgtgaccga
     4321 gcggggcaag atccttcccc ggcgcatcac cgggtcctgt gccaagcacc agcgcgccct
     4381 ggccgtcgcc atcaagcgcg cccgcgccct ggccttcctg cccttcgtct ccaagtaagg
     4441 caagcttccg gcatggaaac caagagcgcc cgaatcccgg aattccgcta tctcgccctc
     4501 ggtgcggtgg cggcattcct gtacggttcc acggtattct cgctgttctt cgcgatgccc
     4561 ctccagacct cctggtcccg gggcggatac cgggcgttct tcgtttccct gtccgtcgcc
     4621 ttggccggca cggcgggatg gctcttctgg cagtaccggg gcctgccctt cggggacctc
     4681 gggggcttcc tcctcatgct gtccctgccc gcgggattgg ccggtgccat ggcccttgcg
     4741 aacgcggggt tcctggcccg cctgcccctc gtataccggg tcatggcggg atccctggtc
     4801 atcgtcgcga tctccctgcc ctcgatctac tcgctgttcg cgagcccgaa cgtccaggcc
     4861 tatctccttc aaggcatcga ggagatgacc cggaccatgg attcggtcgg aggggagggg
     4921 tacgcgggcg cggtcctgcg atccagcctg gaacccgagg cgatgcttca agccgctcgc
     4981 cgggtcatag ccgacgggtt cgccgccgtg ctcttcgggg ccaccttcct aggccactgg
     5041 ctcggtaccc gcgccgcggg cgccgacgcc ccgggctccc ggaaggttcc cgccctgtct
     5101 tcgttttccg ttccgggaca gctgatctgg gcctttttgg gctcctggtc cgccgtcctg
     5161 tccctgaggc ttccgtacct ggccggcctt cggtcggctc cgttcctgga agccctggca
     5221 tggaacgccg ccctgaccgt ctcgctctgc ttcgcggtcc agggactcgg catc
//
LOCUS       DAOTEI010000215         5224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_11453_length_5224_cov_5.152641, whole genome shotgun sequence.
ACCESSION   DAOTEI010000215 DAOTEI010000000
VERSION     DAOTEI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11453_length_5224_cov_5.152641"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="P5313_15270"
     CDS             complement(<1..2008)
                     /locus_tag="P5313_15270"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRY81774.1"
                     /translation="MASVPTGSIELRVDPAGLEALLAFKPDPKGEEWSVGRTMRLALD
                     ARLAGVAQKRIEEILSKFARAPGPASEVLVRGVPPEPPSLEDPEWVELTVPPEAEPFR
                     DAVLREAPAPAVFRIRTEKVSVEKVIRKPGALPFLPAKEERIVEVEKREVREPVALDT
                     RILKVGFTRRGQRLAVLSTAAPGRPGKTVYGKAINPEGPEDTSFHCGEGVTRNRNELV
                     ASADGFVRIGSRWVDIRPFPLHAWEVARSADGTSFLLNFTPGDPRLDLPEAEGILAAA
                     EGLGADRASLVSADDITRAIRSAVAEAEPLLSYSISESRDASVEIVVSPDRMRASVNV
                     RKGRGRGRPLELPALGTAIARAKISGMDTAKVKKDLLEFFKGPETELADYVLAEGRPP
                     GRGKDRELAWSVAFLPEDQAEPLRRAVSAHPALTSSVPTLDDFPLPAAQGVCFVQAGQ
                     EIGKFTPPAPGTPGQSVLGEALSGIPGAEPKIRTFENVARIRDVFRSEVEGLVLRGER
                     DGEVLLRVVPYKEYRAEVTLAPDISEARLSLYPAEGLAPRPTAESLLAALAEEEVSYG
                     IDREAVSGAIQESLEGRPVEGLVVARRKDPVAAGGVRTEWKVRKATGAAVTIREDGRA
                     DYKNQDRSTLVTEGQPLVELVRFGGEGEDGIDVRGQPIKPPAGWE"
     gene            complement(2012..3154)
                     /locus_tag="P5313_15275"
     CDS             complement(2012..3154)
                     /locus_tag="P5313_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRY81775.1"
                     /translation="MHIVPIASGKGGVGKSLVAANLSIALAEAGRRVILADLDLGASN
                     LHVILGIPSTGRGVGTWLSTSGAEFSDIVLSSDYPNLRFIPGDAEIPGLANITAPQKH
                     RLIRKLLALDADYLVLDLGAGTGANILDFFLTATQGIVVTAPALTATLNAYLFLKNAI
                     FRLMWTTFKKGSAAYAVLEGLRKDGSSLQKAYVPAILDQVGRADPESRAAFEAKAAQF
                     RPRLVMNMLEDPKDAEKAQKIRKSSLQYLNLDVEHLGILYRDDLQDIALGSRIPILRY
                     KPQAVLSQAIYRMADKLLSCEDAAEEPLSDAYLDETYQTAEMEAEIDYSAKVEYIEDL
                     LHSGSLTPGDLVETVKSQQFEIGQLRKENQFLKSKLVKAFSQGFKG"
     gene            complement(3163..3627)
                     /locus_tag="P5313_15280"
     CDS             complement(3163..3627)
                     /locus_tag="P5313_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81776.1"
                     /translation="MRRSFITLLGAGGELVRYFGLIALARAFLGGAGGKTTLALLQFA
                     ASPHLLFAGGFFFLWLDSRRYDAFRPLLAAGKALGFLTLGTLLARFALGFRDELPPQG
                     DPAVLLAAMGAFLAWDAAAGLALARSLRAYPLDSGVSRPETPEPVPEPVELE"
     gene            3707..5188
                     /locus_tag="P5313_15285"
     CDS             3707..5188
                     /locus_tag="P5313_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY81777.1"
                     /translation="MQTSPSHGKQDRVAWTAVVAALALCSLLSVSVPPAFAQTQASEA
                     QRYSSLFQNVYSFILQNYVDEVDPKVLYEGAMKGMLDALNDPYSTFLDSAAISDLQDT
                     TVGQYGGIGLYISKTPPSSRRPGEEPWVEVVSPIEDTPGWRAGIQPGDWISEIDGAST
                     APLAMDEVLKKLRGTPGSTVVLTIRRGENITFPVAIVRALIEVPSVKRAMMPNRTGYL
                     RIIEFNPQTTARVKEAVEDFKAQGARAMVIDLRNNPGGLLSAVIDTADLFLDSGVIVS
                     TKSRTAYENQVYRAKSTLTVPRDWPVVVLINRGSASASEILAGALKDQKRAYLIGERS
                     YGKGAVQQIFPLGETGFKLTMSRYYTPSDANIDKIGILPDLEVKEPELTEEESAELSG
                     LLEQRVFANFAKEKPSATEAERRAFVRKLRDSGIKLSDRILDRLLRNELQRTSIAPAY
                     DLEYDIQLQEAIKLIDQGRIRELLSGSRTVRESQDLAAAAPSP"
BASE COUNT          823 a         1827 c         1798 g          776 t
ORIGIN      
        1 tctcccaacc ggcggggggc ttgatcggct ggccccggac gtcgatcccg tcctccccct
       61 cccctccgaa acggaccagt tccaccaggg gctgtccctc ggtgaccagg gtggagcggt
      121 cctggttctt gtagtccgcc cggccgtcct cgcggatggt caccgcggcg cccgtggcct
      181 tccggacctt ccactccgtc cggacgcccc cggctgccac gggatccttg cgccgggcca
      241 cgacgagccc ctccacgggc cggccctcca gggattcctg gatcgccccg gacaccgcct
      301 cccggtcgat gccgtagctc acctcctcct cggccagggc ggccaggagg gattccgccg
      361 tgggccgggg agccaagccc tccgcggggt acagggacag ccgggcctcc gagatgtccg
      421 gggccagggt cacctccgcc cggtactcct tgtaggggac cacccggagc aggacctccc
      481 cgtcccgctc gccccggaga accaggccct cgacctcgct gcggaagacg tcccgtatgc
      541 gggccacgtt ctcgaaggtg cgtatcttcg gttccgcccc ggggatcccc gacagggcct
      601 cccccaggac cgactggccc ggagtgcccg gggcgggggg cgtgaacttc ccgatctcct
      661 ggcccgcctg gacgaagcag acgccctggg ctgccgggag ggggaagtcg tccagggtgg
      721 ggacggagga ggtcagggcc gggtgggcgg agacggccct gcggaggggc tccgcctggt
      781 cctcgggaag gaaggccacg gaccaggcca gctcccggtc cttgccccgt cccgggggcc
      841 ggccctcggc caggacgtag tcggccagtt ccgtctccgg gcccttgaag aactccagga
      901 ggtccttctt gaccttggcg gtgtccatgc cggagatctt ggcccgggcg atcgcggtgc
      961 ccagggccgg cagctccagg ggccgccccc ggccccggcc cttccgcacg ttcacggagg
     1021 cgcgcatccg gtccggggag accacgatct ccacggaggc gtcccggctt tcggagatgc
     1081 tgtaggacag gaggggttcc gcctccgcga ccgcggaccg gatcgcccgg gttatgtcgt
     1141 ccgcggagac cagggaagcc cggtcggcgc ccagtccctc cgcggcggcc aggatcccct
     1201 ccgcctcggg caggtccagg cgcggatccc cgggggtgaa gttgagcagg aaggaagtcc
     1261 cgtccgcgga ccgcgcgacc tcccaggcgt ggagggggaa gggccggatg tccacccagc
     1321 gggacccgat ccggacgaag ccgtccgccg aggcgacgag ctcgttccgg ttccgggtca
     1381 ccccctcgcc gcagtggaag ctcgtgtcct ccggcccctc ggggttgatc gccttgccgt
     1441 acacggtctt cccgggcctg cccggggcgg ccgtggagag cacggccaag cgctggcccc
     1501 tgcgggtgaa tcccaccttc aggatccggg tgtccagggc caccggctcc cggacctccc
     1561 gcttctcgac ctccacgatc cgctcttcct tggcgggcag gaagggcagg gccccgggct
     1621 ttcggatcac cttttccacg gagaccttct ccgtccggat ccggaacacc gcaggggcgg
     1681 gggcttcccg cagaaccgcg tcccggaagg gctccgcctc gggggggacg gtaagctcga
     1741 cccactcggg gtcctccagg ctcggcggct ccggcgggac gccccggacc aggacctccg
     1801 atgcgggccc cggggcccgg gcgaacttgg aaaggatctc ctcgatccgc ttctgggcca
     1861 ccccggccaa gcgggcgtcc agggccaggc gcatcgtccg gccgacgctc cactcctcgc
     1921 ccttgggatc cggcttgaag gcgagaaggg cctccaggcc cgcggggtcg acccgcagct
     1981 cgatcgaacc ggtcgggacg gatgccacga cctacccctt gaatccctgg gagaaagcct
     2041 tgaccagctt ggacttgagg aactggttct ccttccggag ctggccgatc tcgaactgct
     2101 gggacttgac ggtctccacg aggtccccgg gggtcaggga gcccgagtgg aggaggtcct
     2161 cgatgtactc caccttggcg gaataatcga tctccgcctc catctccgcg gtctggtagg
     2221 tctcgtccag gtaggcgtcc gacaggggct cctccgcggc gtcctcgcag gacaggagct
     2281 tgtccgccat gcggtagatg gcctgggaca ggaccgcctg gggcttgtac cggaggatgg
     2341 ggatccggct gcccagggcg atgtcctgga ggtcgtcccg gtagaggatg cccaggtgct
     2401 ccacgtccag gttcaggtac tggaggctgg actttcggat cttctgggct ttttccgcgt
     2461 ccttggggtc ctccagcatg ttcatgacca ggcggggccg gaattgggcg gccttggcct
     2521 cgaaggcggc tcggctctcc gggtccgccc ggcccacctg gtccaggatc gccgggacgt
     2581 aggccttctg gagggaggac ccgtccttcc gtaggccctc caggaccgcg taggccgcgg
     2641 agcccttctt gaaggtggtc cacatgagcc ggaagatcgc gttcttgagg aagaggtagg
     2701 cgttcagggt ggccgtcagg gccggggccg tgaccacgat cccctgggtc gcggtcagga
     2761 agaagtccag gatgttggcc cccgtcccgg cccccaggtc caggaccagg tagtccgcgt
     2821 ccagggccag gagcttccgg atcaggcggt gcttctgggg ggcggtgatg ttggctagcc
     2881 ccgggatctc cgcgtccccg ggtatgaacc ggaggttcgg atagtccgag gaaaggacga
     2941 tgtccgagaa ctccgcgccg gaggtggaga gccaggtgcc caccccccgc ccggtggacg
     3001 gaatccccag gatcacgtgg aggttggagg cccccaggtc caggtccgcc aggatcaccc
     3061 ggcgtcccgc ctccgccagg gctatggaga ggttcgcggc caccagggac ttgcccaccc
     3121 cgcccttccc gctggcgatc ggcacgatgt gcatgggccc tcctactcga gttccacggg
     3181 ctccggcacg ggctccgggg tttccgggcg cgagacgccg gaatccaggg ggtaggcgcg
     3241 gagggaccgg gccagggcga gtcccgcggc ggcgtcccag gccaggaagg cccccatggc
     3301 ggccagcaga accgccggat ccccctgggg cggcagctcg tcgcggaagc ccagggcgaa
     3361 ccgggccagg agggttccca gggtcaggaa accgagcgcc tttcccgcgg ccagcagggg
     3421 ccggaaggcg tcgtacctgc gggaatcaag ccatagaaaa aaaaacccgc ccgcgaacag
     3481 aaggtggggg gaggcggcga actggaggag cgccagggta gtcttacccc ccgccccgcc
     3541 caggaaggcg cgcgccaggg cgatgagccc gaagtaccgg accagttccc cgcccgcgcc
     3601 caggagggtg atgaaggatc gccgcatagg ggtccagtct acccgtggcc tcccgggggc
     3661 gtcaagcatt gacagcgcaa ggcgctgcgc ggacaatagg aatactatgc aaacctcccc
     3721 ctcccacggg aaacaggata gggtcgcctg gaccgcggtc gtcgcggcgc tggcgctgtg
     3781 ctcgctgctc tcggtctccg taccccccgc cttcgcccag acccaggcgt ccgaggccca
     3841 gcggtattcc tccctgttcc agaacgtata ctccttcatc ctccagaact acgtggacga
     3901 agtggatccc aaggtcctgt acgagggggc catgaagggg atgctggacg ccctgaacga
     3961 cccctattcg accttcctgg acagcgcggc catcagcgat ctccaggaca ccacggtcgg
     4021 ccagtacggg ggcatcgggc tgtacatctc caagaccccg ccttccagcc gccgtcccgg
     4081 ggaagaaccc tgggtggagg tcgtgtcccc catcgaggac accccgggct ggcgcgcggg
     4141 gatacagccc ggagactgga tcagcgagat cgacggagcc tccacggctc ccctggccat
     4201 ggacgaagtc ctgaagaagc tccgcggaac cccgggctcc acggtggtcc tgaccatccg
     4261 caggggcgag aacatcacct tccccgtggc gatcgtccgc gccctgatcg aggtcccctc
     4321 ggtcaagcgg gcgatgatgc ccaaccgcac gggctacctg cgcatcatag agttcaaccc
     4381 ccagaccacc gcgcgggtca aggaagccgt ggaggacttc aaggcccagg gcgcacgggc
     4441 catggtgatc gacctccgga acaaccccgg aggccttctg tccgcggtca tcgacaccgc
     4501 cgacctcttc ctggattcgg gtgtcatcgt ctccacgaaa tcccggacgg cctacgagaa
     4561 ccaggtctac cgcgcaaaat ccaccctgac cgtgccccgg gattggcccg tggtggtcct
     4621 catcaatcgg ggctccgcca gcgcctcgga gatcctggcg ggggccctga aggaccagaa
     4681 gagggcctac ctgatcgggg agcggagcta cggcaaggga gcggtccagc agatcttccc
     4741 cctgggggag acggggttca agctgaccat gtcccggtac tacaccccca gcgacgccaa
     4801 catcgacaag atcggcatcc tgccggacct ggaggtgaag gagccggagc tcacggagga
     4861 ggagtccgcg gagctgtccg ggctcctgga gcagcgggtc ttcgccaact tcgccaagga
     4921 gaagccctcc gccacggagg cggagcgcag ggccttcgtc cggaagctcc gggactcggg
     4981 tatcaagctt tcggaccgga tcctggaccg gctcctccgc aacgagctcc agcgcacttc
     5041 catagccccg gcctacgacc tggagtacga catccagctc caggaggcga tcaagctgat
     5101 cgaccagggc cggatccggg agctcctgtc cggatcccgg acggtccggg aaagccagga
     5161 cctggcggcc gcggctccgt cgccctgagc gcgcggattc cgcgcggaaa ggcgatatgc
     5221 gtca
//
LOCUS       DAOTEI010000216         5193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_11538_length_5193_cov_5.030362, whole genome shotgun sequence.
ACCESSION   DAOTEI010000216 DAOTEI010000000
VERSION     DAOTEI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11538_length_5193_cov_5.030362"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="P5313_15290"
     CDS             <1..834
                     /locus_tag="P5313_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRY81778.1"
                     /translation="DYPEDALAYRGGFERCGDQIAAVRDSFDLESILACNPETGTWIV
                     DEAAFYDERVAYLMRDESEKRGLLFICPTLVLNFRREIFNQTARLLLETATDVIPLTA
                     YCEHPDCIADSLYTYRHYAVDGIECPAPYFDPLIIIGGDRRKDDPREPNYCTRCDRHH
                     FLPGKEYTYFTLKPLGEAASRGDLKPLLIELAALAGNVEGSRLAASLRKRHLDREDPE
                     PENMETLRVPCLAERALVFLYAEANLLSLEQLRKVAADLGLDRDYLAGRLADNRRPVE
                     F"
     gene            complement(854..1162)
                     /locus_tag="P5313_15295"
     CDS             complement(854..1162)
                     /locus_tag="P5313_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRY81779.1"
                     /translation="MSRIERELHTLEVMIGMYCRRRHGTRKADACGECKALLGYARER
                     LRSCRYGEAKPVCSRCRTHCYRADMRARIREVMGWAGPRMLFRHPRLAILHLADSRRR
                     "
     gene            complement(1280..2467)
                     /locus_tag="P5313_15300"
     CDS             complement(1280..2467)
                     /locus_tag="P5313_15300"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HRY81780.1"
                     /translation="MGTRKLYYEDTERFECEATVIARTELAGRHAVVLDATVFYPEGG
                     GQPCDFGTIDGIPVVDVQERDGVVLHVLASPLPERGGPVPCRVDADRRRDHMEQHTGQ
                     HLLSSRVLKLAGGGATVSFHLGQEYSTVDVDLPELSREAADRIEDEIARIIREGHPVT
                     IHVCPPENVSDFPLRRPPPAGEEALRIVDLDGLDYSACCGTHLADTGRIGALRILRTE
                     KYKGMTRIYFAAGERARRDARRTAALTRETARLLGCSEDEAPDRVARSLERIRELEVR
                     LSIARAERAAAEARVFLADAPASGLLVLPLSDRTYDEVLEAAKAVTGLSGRPAAAASR
                     PDLRAVVTAPDASLRLGERLKPLLAGLGGKGGGGPAQFQAQFPDEASLTAFLEAARGI
                     LAG"
     gene            2605..3702
                     /locus_tag="P5313_15305"
     CDS             2605..3702
                     /locus_tag="P5313_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRY81781.1"
                     /translation="METGDIRVRRGELEAFCGSVFEHRGMGPGDAAVTAEVLVAADAC
                     GIPSHGVGRLRRYVSGLDTGLMLPRETGTTILESPASLVIDARGAMGPPVSVRTMDAV
                     IAKARENGAAFGTVRNSNHFGIAGFYARRALSEDMLGIAMTNTAALGVPTFGREAFFG
                     TNPIAFAAPAGEEGAFVLDMSTTVVTRGKVEVYERKGGELPEGWAVDHTGKTARDPGS
                     LLEGMQERRGGGILPLGGLDTLLGGHKGYGLAVMVDILCAVLSGGPFGRDVYDTPESS
                     ARVSHFFGAIRIDRFRDPGEFRADMDRMLRDLRACPPAEGRDRVWFAGLPEAEAERES
                     KRRGVPLSRKTWEVLRGLAVETGVHLPREIP"
     gene            complement(3911..4795)
                     /locus_tag="P5313_15310"
     CDS             complement(3911..4795)
                     /locus_tag="P5313_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY81782.1"
                     /translation="MPSFEALDRLSVTVVAEDTVMYESPCWGQHGISFFVTAVKGGVT
                     RNILIDTAQDPVALLHNMDLLGIRPSGIDGIVITHCHVDHTGGLSEVLKAVGKKDLPV
                     VAHPDLFRPNFVEEPFLEHVGISAGNTRARIEAAGGLLLLTTDPLKLMTGLSTTGYVT
                     RRTDFEEVGLPLRTLDGEGRLVQDPMKDDISVAAVLPGGKLVILTGCSHAGIVNIARH
                     SLELTGQKGIRAILGGLHLVEAPLDRIRKTADALAGLPIESVYAGHCTGFEAQVELRK
                     TFGDRFRPLQTGMRFEFP"
     gene            complement(4806..>5193)
                     /locus_tag="P5313_15315"
     CDS             complement(4806..>5193)
                     /locus_tag="P5313_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81783.1"
                     /translation="APATSGPPAAAPTPLSGSALPPDAAPLPPRDLPSSPEELLRLAR
                     EDLAASRIAGVLEVLDRYMLLFPFGSDEAHYLYAKAYEQNTPYRDIKKAYSNYRRVRD
                     EWPRSPFWKESAERVSYIERHYFDIR"
BASE COUNT          828 a         1761 c         1802 g          802 t
ORIGIN      
        1 gactaccccg aggacgccct ggcctaccgg gggggcttcg agcgctgcgg ggaccagatc
       61 gcggcggtac gggactcctt cgacctggag agcatcctgg cctgcaaccc cgagacgggg
      121 acctggatcg tggacgaagc ggccttctac gacgagcggg tggcctacct catgcgggac
      181 gagtccgaga agcgcggact gttgttcatc tgcccgaccc tggtcctaaa cttccggagg
      241 gagatcttca accagaccgc ccggctcctc ctggagaccg ccacggacgt gatccccctg
      301 accgcctact gcgaacaccc ggactgcatc gccgactccc tgtacaccta ccggcactac
      361 gccgtggacg gcatcgagtg cccggccccc tacttcgatc ccctgatcat catcgggggg
      421 gaccgccgga aggacgatcc ccgggagccc aactactgca cccgctgcga ccgccaccac
      481 ttcctgccgg gcaaggagta cacctacttc accctgaagc ccctggggga ggctgcctcc
      541 cggggggacc tgaagcccct cctgatcgag ctggccgccc tggccgggaa cgtcgaaggt
      601 tcccgcctgg ccgcctccct ccggaagcgc cacctggacc gggaggatcc ggagcccgag
      661 aacatggaga ccctccgggt gccctgcctg gcggagcgcg ccctcgtctt cctctacgcc
      721 gaggcgaacc tgctatccct ggaacagctg cgaaaggtgg ccgcggacct gggcctggat
      781 cgggactatc tggccggccg actggccgac aaccgccggc ccgtggaatt ctagcggggt
      841 tcggcggtcg cccctatcgc cttcgggaat ccgccaggtg caggatcgcc agccggggat
      901 ggcggaacag catccggggc cccgcccagc ccatgacctc gcggatccgg gctcgcatgt
      961 ccgcccggta gcagtgggtc cggcaccggg agcagaccgg cttggcttcc ccgtagcggc
     1021 aggatcgaag cctctcccgg gcgtagccga gcagggcctt gcattccccg cacgcgtccg
     1081 ctttccgcgt cccatggcgg cggcggcagt acatcccgat catgacttcc agggtatgaa
     1141 gctcgcgctc gatgcggctc actcgatctt tcctcctggg cagccccggc ggtgccgggt
     1201 tgaggtctag aaaattgcgc ccggtgagcc gcgccccgat ccgagcccgg aatccacggc
     1261 tacgaaaatg cgcctgtccc tagcccgcca ggatcccccg ggcggcttcc aggaaggcgg
     1321 tcagggacgc ctcgtccgga aactgggcct ggaactgggc ggggcctccc ccgcccttcc
     1381 ccccgaggcc ggccagcagg ggcttgaggc gctcccccag gcggagggac gcgtccggag
     1441 ccgtgaccac cgcccgcagg tccggccggg aggcggccgc ggcggggcgg ccggagagcc
     1501 cggtcacggc cttggccgcc tccaggacct cgtcgtaggt ccggtccgag aggggcagga
     1561 ccaggagacc cgaggccggg gcatcggcca ggaagacccg ggcttccgcg gccgcccgtt
     1621 ccgcccgggc gatagacagc cggacctcca actcccggat ccgctccagg ctccgggcga
     1681 cgcggtccgg ggcttcgtcc tcggaacagc cgagcaggcg ggccgtctcc cgggtcaggg
     1741 cggcggtcct gcgggcgtcc cggcgggccc gttccccggc ggcgaagtag atccgggtca
     1801 tgcccttgta cttctcggtc ctgagtatgc gcagggcccc gatccggccc gtgtccgcca
     1861 ggtgggtccc gcagcacgcg gaatagtcca ggccgtccag gtccacgatc cggagggcct
     1921 cctctcccgc cggcggcggg cgtcggagcg gaaagtccga aacattttcc ggagggcaca
     1981 catggatggt caccgggtgt ccctcccgga tgatccgggc gatctcgtcc tcgatccggt
     2041 ccgcggcctc gcgggaaagt tccggaaggt ccacgtcgac ggtggagtat tcctgcccca
     2101 ggtggaagga gacggtggct ccccccccgg cgagcttgag cacccgggac gagaggaggt
     2161 gctgtcccgt gtgctgctcc atgtggtccc ggcgccggtc cgcgtccacc cggcacggga
     2221 ccgggccgcc ccgttcgggc aggggcgagg ccaggacatg cagcacgacc ccgtcccgct
     2281 cctggacgtc gacgaccgga atcccgtcga tcgtcccgaa gtcgcagggc tggccgcccc
     2341 cctcgggata gaagaccgtc gcgtccagca cgaccgcatg tcggcccgcg agctcggtcc
     2401 gcgcgatcac ggtcgcctcg cactcgaacc gttccgtgtc ttcgtagtac agcttccggg
     2461 tacccatggg aggattctag gccgccggag gctgcctcgt aaagcgcccg aaaggcggaa
     2521 atttccagga aacgcaagaa tttcctcctc ccggggacgg gcggatcggg tatgctggat
     2581 acgagaacac ggcaaggagc atccatggaa accggggaca tcagggttcg gcgcggggag
     2641 ctggaagcct tttgcgggtc cgtcttcgag caccggggca tgggccccgg ggacgcggcc
     2701 gtgaccgcgg aggtcctcgt ggccgcggac gcctgcggga ttccctccca cggggtggga
     2761 cgcctgcggc gctacgtgag cggactggac acgggtctca tgctgccccg ggaaacgggg
     2821 acaaccatcc tggagagccc cgcttccctg gtcatcgacg cccggggcgc gatgggcccc
     2881 cccgtgagcg tccggacgat ggacgcggtc atcgccaagg cccgggagaa cggtgcggcc
     2941 ttcgggacgg tgcgtaactc caaccacttc gggatcgccg ggttctatgc ccgcagggcc
     3001 ctgtccgagg acatgctggg catcgccatg acgaacacag cggccctggg ggtgcccacc
     3061 ttcggccggg aagccttctt cgggaccaac cccatcgcct tcgccgcccc cgccggggag
     3121 gaaggcgcct tcgtcctgga catgtccacc accgtggtga cccggggcaa ggtggaggtg
     3181 tacgaacgca agggcgggga actcccggaa ggctgggccg tggaccacac gggtaagact
     3241 gcccgggatc ccgggtccct cctcgagggc atgcaggagc gccgaggggg cggaatcctg
     3301 cccctgggcg gactggacac cctgctgggc ggacacaagg gctacgggct ggcggtgatg
     3361 gtggacatcc tctgcgcggt gttgtccggc gggcccttcg gccgggacgt gtacgatacc
     3421 cccgagtcct ccgcccgggt ctcgcacttc ttcggggcga tccggatcga taggttccgg
     3481 gaccccggag aattccgggc cgacatggac cggatgctcc gggacctacg ggcctgcccg
     3541 cccgcggaag gccgggaccg ggtgtggttt gcgggcctgc ccgaggcgga agccgagcgg
     3601 gagtccaagc gccggggcgt gcccctgtcc cgcaagacct gggaggtgct ccggggcctg
     3661 gccgtggaga cgggggttca ccttccgcga gaaattcctt gagtggagat ctcgggacct
     3721 gttcatacgc tggtatcaga atggtcagaa acttggaacc ctagaacaga caggatgaac
     3781 aggatcacat atggatttac aagatgggta tatacataga taggtattcc ttcatcctgt
     3841 tcatcttgtc ttgatcctgt acatcctgtc aaagatttga gtagaaaccg tggatttccg
     3901 cctcaccgga tcatggaaac tcgaacctca tccccgtctg gaggggccgg aagcggtccc
     3961 cgaacgtctt ccgcagctcc acctgggctt cgaagcccgt gcagtgtccc gcatagaccg
     4021 actcgatcgg gagaccggcc agggcgtccg cggtcttccg gatccggtcc aggggggcct
     4081 ccaccaggtg cagccccccc aggatggccc ggatcccctt ctgccccgtg agctccaggg
     4141 aatgccgggc gatgttcacg atccccgcgt ggctgcagcc cgtcaggatc acgagctttc
     4201 cgcccgggag gaccgcggcc accgagatgt cgtccttcat gggatcctgg accagccggc
     4261 cttccccgtc gagggtgcgc aggggcaggc cgacctcctc gaagtccgtc cggcgcgtca
     4321 cgtacccggt ggtcgacagg cccgtcatga gcttcagggg gtccgtggtc aggaggagga
     4381 ggcctccggc cgcctcgatc cgagcccgtg tattcccggc ggagatcccg acatgctcca
     4441 ggaagggctc ctcgacgaag ttgggacgga aaaggtccgg gtgcgcgacc acgggaagat
     4501 ccttcttccc gacggccttc agcacctccg aaaggcctcc ggtgtggtcg acgtggcagt
     4561 gggtgatcac gatcccgtcg attccggacg ggcggatccc cagcaggtcc atgttgtgaa
     4621 ggagggcgac gggatcctgg gccgtgtcga tcaggatgtt ccgggtcacc ccgcccttca
     4681 ccgccgtgac gaagaaggag atgccgtgct gaccccagca ggggctctcg tacatcaccg
     4741 tatcctccgc gacgacggtc acggagagtc ggtccagggc ttcgaaggac ggcatgccgg
     4801 gcctcctaac ggatgtcgaa atagtgccgc tcgatgtagg acacccgttc cgcggattcc
     4861 ttccagaacg gggaccgggg ccactcgtcc cggaccctcc ggtagttgga gtaggccttc
     4921 ttgatgtccc gatagggagt gttctgctcg taggccttgg cgtacaggta gtgggcctcg
     4981 tcggaaccga aggggaatag gagcatgtag cgatccagga cctccaggac cccggcgatg
     5041 cgggaggcgg ccaggtcctc ccgggccagg cgcaggagct cctcgggact cgagggcagg
     5101 tcccggggcg ggaggggagc ggcgtcgggg gggagagcgg agcccgaaag gggcgtcggg
     5161 gccgccgccg ggggtccgga cgtcgccggg gcg
//
LOCUS       DAOTEI010000217         5149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_11679_length_5149_cov_3.625638, whole genome shotgun sequence.
ACCESSION   DAOTEI010000217 DAOTEI010000000
VERSION     DAOTEI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11679_length_5149_cov_3.625638"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1441)
                     /locus_tag="P5313_15320"
     CDS             complement(158..1441)
                     /locus_tag="P5313_15320"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRY81784.1"
                     /translation="MKRRGPGRHATLERYILGEFLLAFSVSFAFFFLVFFANQMLLMA
                     EDILAKRAPVWDVAKLIFYATPAVLALAFPFASLTGALMAAGRLSADSELLVLQSAGV
                     SPRRIFLPFLVLGALFSAGSFVMNDALLPLGTVNFSRLYRRILAVSPALELTPHSACR
                     WEGLTIVSGDSRDGVVRDLLILDTPQGGKKRIISAGSGTLSEGEPGVLTLSLQDVFSQ
                     SNDPARPGRFEWSKAESLQYNLLLRDISDMSLGVSPREMSSRDLGALIRERQKAAEAR
                     SVRRSEEARDAREQAADAYLKALVRRTPAGQAVSELRPLLERVAGLSVPAAEERTLRI
                     YRLEYYKKFSIPAGAFCFVILAFPLATLTRRGGRAAGFGIGLLVAVIYWAMLAGGQTL
                     GNRIEGISPFWAMWAPNVFVLAAGGAAFATKRVMG"
     gene            complement(1450..1893)
                     /gene="dut"
                     /locus_tag="P5313_15325"
     CDS             complement(1450..1893)
                     /gene="dut"
                     /locus_tag="P5313_15325"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009805695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRY81785.1"
                     /translation="MEVLSVRITSEDGARLPEYKSDGASGADLRAHLAEPLVLEPGGR
                     TAVPTGLRMEFPEGWEAQVRPRSGLALEHGITCLNSPGTVDSDYRGEIRVILANLGTE
                     PFTVRPGDRIAQLVFAPVVRASFTRVESLTDSGRGGGGFGSTGRA"
     gene            complement(1896..3164)
                     /locus_tag="P5313_15330"
     CDS             complement(1896..3164)
                     /locus_tag="P5313_15330"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY81786.1"
                     /translation="MEIRQSRRPSGAAAVLEPVPASHSVTIGFWFPLGSGMEMDSERG
                     YTHFVEHMLFKGTGTRDARTLARQAERAGGYLNAFTEKNTLCVHCTLPADRWADAVEL
                     LADMAFRSVFDPAEFLRERDVIRSEVLAARDDPEEDAADEFHARVWSGHPLARRVAGE
                     PADVDRVDRDGLFRFYRDYLVPGNLTVSAAGNFDPERLASALDAALEGVPAGRPVPAT
                     SGPEFHPGSWCRSAPAGQVYLVSGFELPPGLAREELAALDALNCAFGESSSSRLFQNI
                     REARGLCYSIGSSYASAREYGMFYVTAVSAPSNFPRLWDMIRSELDGLHMSGLTEEEV
                     AEAVAHIHGQDMVAADDTETRMRTLARQWMRFGRAEPYETFCRGILGVDPERVRAVRD
                     LIFRGSSGALAYGRIGARIRNSLGADENGG"
     gene            complement(3225..>5149)
                     /gene="pnp"
                     /locus_tag="P5313_15335"
     CDS             complement(3225..>5149)
                     /gene="pnp"
                     /locus_tag="P5313_15335"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRY81787.1"
                     /translation="PGGFIKRETRPKDREILVSRIIDRPMRPLFDKAFGREIQVVPTC
                     ISTDQVNPPDIVGINAASAAVHISDIPFAGPIAAVRVCHVDGQFVINPTFDQIGRSQL
                     EIIVAGTKDGITMVEGDASEVAETLMIEAIERARGPISDICAMIEKLRSEAGKPKLPL
                     APMSVQLARKDEIAAMAREKLSKALFTKVKQERGAAVSAAIKETLAAFPQVSEDDVQK
                     KLFNALMEDLQYEILRSGILDRGTRVDGRGLEDIRPITCEIDILPRTHGSALFTRGET
                     QALAVTTLGTVFDEQILDDIEGDRRDRFMLHYNFPPFSVGEVGRLGTGRREIGHGNLA
                     RRSLEKVLPPKEKFPYTVRVVSEILESNGSSSMASVCGGSLSMMAAGVPLKKPVAGIA
                     MGLITEGSRFAVLSDILGDEDHLGDMDFKVAGTENGITGFQMDIKISGVSTEILTKAL
                     DQARRGRMHILGIMNQALPAPASDISEYAPKIVSLHVNPEKIGAIIGPGGKNIKALCE
                     MYSVEINIEDDGSCTVYGKQQKNAYEARDAILAIVEDPEVGKIYHGTVKKIMDFGAFV
                     EIMPGKEGLCHISRLARHRVNAVTDVLKEGQKVDVKLLEVDKMGRLNLSYIDALEDSG
                     ASKGEGPAPSAPPHRP"
BASE COUNT          867 a         1667 c         1785 g          830 t
ORIGIN      
        1 ctgggccgac gacgcctcgt tctggatgta cttcacgagg ttggcgaaga ggtcgatcag
       61 ttcgaagagc agggagaaaa acgccaccgc tgccaggaag gccggaacga cgttggccag
      121 gacataggcc tggagggtcc acccggtctt cacgcgccta ccccatcacc cgcttggtcg
      181 cgaaggcggc tccgccggcg gccaggacga agacgttcgg cgcccacatg gcccagaagg
      241 gtgagatgcc ctcgatgcgg ttgcccaggg tctgcccccc cgccagcatg gcccagtaga
      301 tcacggccac cagaagcccg atgccgaacc ccgcggcccg gccgccccgg cgggtgaggg
      361 tggccagagg aaaggccagg atcacgaagc agaaggctcc cgcgggtatc gagaacttct
      421 tgtagtactc caagcgatag atcctgaggg tgcgctcctc cgcggcgggg accgagagtc
      481 cggctacccg ctccagcagg ggcctcagct ccgagaccgc ctggccggcg ggtgtcctgc
      541 ggaccagggc cttcaggtac gcgtccgccg cctgttcccg ggcgtcccgg gcctcctcgg
      601 aacgccggac ggaccgggct tccgcggcct tctgccgctc ccggatcagg gctcccaggt
      661 cccgggagct catctcccgg gggctcacgc ccaggctcat gtcggaaatg tcccgcagaa
      721 ggaggttgta ctgcagggat tccgccttcg accactcgaa ccggcccggc cgcgcgggat
      781 cgttcgactg gctgaagaca tcctgcaggg acagggtcag gaccccgggt tccccctcgg
      841 agagggttcc ggagcccgcc gagatgatcc gcttcttccc tccctggggg gtgtccagga
      901 tcaggagatc ccgcacgact ccgtcccggg agtccccgga cacgatggtc aggccctccc
      961 acctgcaggc cgaatgcggg gtcagctcca gggcgggaga cacggccagg atcctgcggt
     1021 acagccggct gaaattcacg gtacccagag gaaggagcgc gtcgttcatc acgaaggagc
     1081 cggccgagaa aagggccccc aataccagga agggcaggaa aatacgccgg ggggagaccc
     1141 ccgcggactg gagcaccagg agttcggaat ccgcggaaag gcggcccgcg gccatcaagg
     1201 cccccgtcag ggaggcgaag ggaaaggcca gggccaggac cgccggggtg gcgtagaaga
     1261 tcaatttcgc cacgtcccag accggagccc gcttggccag gatgtcctcc gccatgagga
     1321 gcatctggtt ggcgaagaag accaggaaga agaaggcgaa ggaaacggag aaggccagga
     1381 ggaattcccc caggatgtac ctctccaggg tagcgtgtcg tcccgggccc cgccgcttca
     1441 tcgggaatct caggcccgcc ccgtggagcc gaatccgccc ccgccccggc cgctatctgt
     1501 gagtgactca acccttgtga aggaagcccg tacgacgggg gcgaagacga gctgggcgat
     1561 ccggtccccg ggccggacgg tgaaaggctc cgttccgagg ttggccagga tgacccggat
     1621 ctcgccccgg tagtccgagt cgacggtgcc cggggagttc aggcaggtga tgccgtgttc
     1681 cagggccagc ccggatcggg gccgcacctg ggcttcccac ccctccggaa actccatgcg
     1741 gagccccgtg gggaccgcgg tccgtcctcc gggctcaagg acgaggggtt cggctagatg
     1801 ggcgcgcaga tccgcaccgg aggccccgtc cgacttgtat tccggcagcc gggctccgtc
     1861 ctccgaggtg atgcgcacgg acaggacttc catcctcatc ctccgttttc gtccgcgccg
     1921 agggagttcc ggatccgggc gccgatccgg ccgtaggcca gggcacccga agaaccccgg
     1981 aagatcaggt cccggacggc ccggacccgc tccggatcga cccccaatat cccgcggcag
     2041 aaggtttcgt acggttccgc ccggccgaac cgcatccatt gacgggccaa ggtccgcatc
     2101 cgggtttccg tgtcgtccgc ggccaccata tcctgaccgt ggatgtgggc caccgcctcg
     2161 gcgacctcct cctccgtgag cccggacatg tgaaggccgt cgagctcgct ccgtatcata
     2221 tcccacaatc ggggaaaatt ggagggagcg ctgaccgcgg tgacgtagaa cataccgtat
     2281 tcccgggcgc tcgcgtagga ggatccgatg gagtagcaca acccccgggc ttcccggatg
     2341 ttctggaaaa gccgggaact ggaggactcc ccgaaggcgc agttgagggc gtccagggcc
     2401 gccagctcct cccttgcaag accggggggc agttcgaagc ccgagaccag gtagacttgg
     2461 cccgcggggg cggaacggca ccaggaaccg ggatggaatt ccggtcccga ggtggccggg
     2521 accggccggc cggccgggac accctcgagg gcggcatcca gggccgatgc cagccgctca
     2581 ggatcgaagt tgcccgcggc ggagacggtc aggttgccgg gaacgaggta gtcccggtag
     2641 aagcggaaca atccgtcccg gtcgaccctg tccacgtccg cgggctcacc ggccacccgc
     2701 cgtgcaaggg ggtgtcccga ccagacccgg gcatggaact cgtccgcagc gtcctcctcg
     2761 ggatcgtccc gggccgccag gacctcggaa cggatcacgt cccgctcgcg caggaattcc
     2821 gcggggtcga agacggaacg gaaggccatg tcggccagga gttctaccgc gtcggcccat
     2881 cggtccgcgg gcagggtgca gtggacgcag agggtgttct tttcggtgaa tgcgttgaga
     2941 tagcccccgg cccgctcggc ttgccgcgcc agggtccgtg catccctcgt gccggtgccc
     3001 ttgaagagca tgtgctcgac gaagtgggtg tagccgcgct cggaatccat ctccatcccg
     3061 gaacccaggg ggaaccagaa cccgatggtc acggagtggg acgcgggtac gggctcgagc
     3121 actgcggccg cgccgctcgg tcttcgggat tgacggattt ccatgcacgg cctcgatgac
     3181 aaagaggcgg ccgggatcgc cccggccgcc tggttatttt ccgcttacgg gcggtgcggg
     3241 ggcgcggaag gagcgggacc ctcgcccttc gaggcgcccg aatcctccag ggcgtcgatg
     3301 tacgagaggt tcaggcggcc catcttgtcc acctcgagga gcttgacgtc caccttctgg
     3361 ccttccttga gcacgtccgt gacggcgttg acccggtggc gggcaagacg ggagatgtgg
     3421 cacaggcctt ccttgccggg catgatctcg acgaaggcgc cgaaatccat gatcttcttc
     3481 acggtaccgt ggtagatctt cccgacctcg ggatcctcca cgatggccag gatggcgtcc
     3541 cgggcctcgt aggcgttctt ctgctgcttg ccgtagaccg tgcaggatcc gtcgtcctcg
     3601 atgttgatct ccacggagta catctcgcag agggccttga tgttcttgcc gccggggccg
     3661 atgatggcgc cgatcttctc gggattgacg tggagggaga cgatcttcgg ggcatactcg
     3721 gagatgtccg aggccggcgc ggggagggcc tggttcatga tgcccaggat atgcatccgg
     3781 ccgcgccgcg cctggtccag ggccttggtc aggatctccg tggaaacccc ggaaatcttg
     3841 atgtccatct ggaagcccgt gatcccgttc tccgtcccgg ccaccttgaa gtccatgtcg
     3901 cccaggtgat cctcgtcgcc caggatgtcc gaaagcacgg caaagcggct gccctcggtg
     3961 atgaggccca tggcgatgcc cgccacgggt ttcttgaggg gcaccccggc ggccatcatg
     4021 gaaagggagc cgccgcacac cgaggccatg gaggaggagc cgttggattc caggatctcg
     4081 gagaccacgc ggacggtgta ggggaatttt tccttcgggg gaaggacctt ttccagggag
     4141 cggcgggcga ggttcccgtg cccgatctcc cgccggccgg tgccgagccg tcccacctca
     4201 ccgaccgaga agggggggaa gttgtagtgg agcatgaacc ggtcccgccg gtcaccctcg
     4261 atgtcgtcca ggatctgctc gtcgaagacg gtgcccaggg tggtgaccgc cagggcctgg
     4321 gtttcccctc gggtgaagag ggccgatccg tgggttcggg gcaggatgtc gatctcgcag
     4381 gtgatgggcc ggatatcctc caggccgcgg ccgtccaccc gggtcccgcg gtccaggatg
     4441 ccggagcgga ggatctcgta ctggaggtcc tccatgaggg cgttgaagag cttcttctgg
     4501 acgtcatcct ccgagacctg ggggaaggcg gccagggtct ccttgatggc cgcggagacg
     4561 gcggctccgc gctcctgctt gaccttggtg aacagggcct tggagagctt ctcccgggcc
     4621 atcgccgcga tctcgtcctt ccgggccagc tgcacggaca tgggcgcgag gggcagcttg
     4681 ggcttgcccg cctcggaccg gagcttctcg atcatggcgc agatgtccga gatgggtccc
     4741 cgggcgcgct cgatggcctc gatcatcagg gtttcggcga cctcggaggc atccccctcc
     4801 accatggtga ttccgtcctt ggtcccggcc acgatgatct cgagctggct gcggccgatc
     4861 tggtcgaagg tgggattgat gacgaactgg ccgtccacgt ggcagacccg caccgccgcg
     4921 atgggacccg cgaaggggat gtccgagatg tggaccgcgg cgctggccgc gttgatcccg
     4981 acgatgtccg gaggattgac ctggtccgtg gagatgcagg tcggtacgac ctggatctct
     5041 cggccgaagg ccttgtcgaa gagggggcgc atgggccggt cgatgatcct cgagacgagg
     5101 atctcgcggt ccttgggcct ggtctcgcgc ttgatgaagc cgccgggga
//
LOCUS       DAOTEI010000218         5063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_11936_length_5063_cov_4.418331, whole genome shotgun sequence.
ACCESSION   DAOTEI010000218 DAOTEI010000000
VERSION     DAOTEI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11936_length_5063_cov_4.418331"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /gene="nusA"
                     /locus_tag="P5313_15340"
     CDS             complement(<1..1158)
                     /gene="nusA"
                     /locus_tag="P5313_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRY81788.1"
                     /translation="MSSEMAEAIRQLIYEKGISEDLVIKTIEDSLLAAYKKRYGTSEN
                     AVVRFRENYEGVDIFAKKTIVEELDDPVLEITLDEARAYSEEAEIGDILELPVDPREF
                     DLQSVMLAKQTTRQNLREISRDTLYAEYKSKVGEIIIGYYQRDRNGNIYVDLGRVEGV
                     LPKKYQSPRETYHPNDRIKAYIVEVNKTKTGPQIVLSRTHAEFVRKILELEVPEIYDN
                     IVEVFKIVREPGYRTKISVFSKREDVDPVGACVGLKGIRIQSIIRELEGEKIDVLKYD
                     IDPKRFIKNALSPADVKEVFILDEPKRMALAVVPDNQLSVAIGKQGLNVRLANRLCDW
                     NIDVKTEEQFYQMDRSVDLKRAVSELFTEEEEEITRLSELPGVPESAVAALK"
     gene            complement(1163..1609)
                     /locus_tag="P5313_15345"
     CDS             complement(1163..1609)
                     /locus_tag="P5313_15345"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81789.1"
                     /translation="MESDRYRKDLEELLSGAGLGLVDLSVSRHRGSVQVRAVIYAPGG
                     TGIDECSRAHRLIQPRLQVLLSEQDFHLEVASPGVDRWIRSDREYAIFTGRGIRILLK
                     EGDEWLAGRIAQAGPSAMTIRGAWGERTVEYASIAKARLDYAQEGA"
     gene            complement(1792..2475)
                     /locus_tag="P5313_15350"
     CDS             complement(1792..2475)
                     /locus_tag="P5313_15350"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRY81790.1"
                     /translation="MSRALEILFPRCCVLCGRGLLEEADRAYPLCGECEEDLPRIRGE
                     RCPICGKPLVSERGLCMRCRDRSFAFDSAYPLWEYAGRIKDLLISYKIRGVRPLARFL
                     ASRVYDRLREGYPGTVLVPVPFRKGKIRKTGWDQVEDLARSLERRGVPVMRCLERAEG
                     ASQKTLDYSARMSNLTGKIRVRKGAKAPARVVLLDDVLTTGATLSECARVLKAAGTRR
                     VDALVLAAD"
     gene            complement(2537..>5063)
                     /gene="mutS"
                     /locus_tag="P5313_15355"
     CDS             complement(2537..>5063)
                     /gene="mutS"
                     /locus_tag="P5313_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRY81791.1"
                     /translation="HRDEILFFRLGDFYEMFERDALEVSSLLNLTLTKRQDAPMCGIP
                     YHAAHSYISRLLKAGKKIAICEQTAVAGVSRGLMDREVVEIITPGTTVEEDYLDSRSN
                     NYLVSIGRVRDVLGIAYVDVSTGEFCAFSAKQDAPARLRKELYRLAPREALVQQTLLD
                     DPDIAQILQEREGLILNRFPDWSYDQAQASEILTKRFGTASLKGFGFSDDDPALAPAG
                     VLVRYLEDAFRGAVPHLEGLRPRREEAFADIDESAQRNLEIDRNLQDGGRAFTLLDVL
                     DATRTAMGARLLKHWLLRPLRDPAAIRSRLDAVERLYRNQRTLERLRSCLGNCMDLER
                     LASRVAMDKAHAKDLLGIKGSLDTIREAAALLEPETGAAPELGLDLGPEVEEELRSVS
                     DLVGRAILEDPSVLLNEGNLIRPGFDPELDALRDLRDSSRSVLESYLEEERTATGIQN
                     LRIRYNRILGYYLEVTKGALPQVPSHFIRRQSLSTGERYTTDRLAELESRINGAQERI
                     VERERERFLEVRTEVKAAVPRLLEAARACARVDCLASFAYTATARGYTRPEVDDTGTL
                     GILEGRHPVVEAHRAGGDFVPNDVRLDSGEVSFALITGPNMAGKSTYLRQTALIVLLA
                     QAGSFVPAGEARIGVVDRIFCRVGAQDNLARGESTFLVEMHETAYILNNAGPRSLVIM
                     DEVGRGTGTLDGLAIAWAAAEHLLDAVGCRTLFATHYHELTALEHPKLVNRSLAVRES
                     AGEVVFLKRVVEGPAAGSYGLHVAGLAGIPAPVLARARQVRENLARKERDLPAAVASP
                     RQPAQAALFSNEELVLEELRSLDPDRITPLEALSRLARLKKLL"
BASE COUNT          822 a         1655 c         1665 g          921 t
ORIGIN      
        1 cttcagggcg gccacggcgc tttcgggcac tcccggcagc tcggagagcc gcgtgatctc
       61 ctcctcctcc tcggtgaaga gttcggagac cgcgcgtttt aagtccacgg agcggtccat
      121 ctggtagaac tgctcctcgg tctttacgtc gatgttccag tcgcacagcc ggttggccag
      181 ccgtacgttc aggccctgct ttccgatggc gacggacagc tggttgtccg ggacgaccgc
      241 aagggccatg cgcttgggct cgtccaggat gaagacttcc tttacatccg ccggggacag
      301 ggcgttcttg atgaaccgtt tgggatcgat gtcgtacttg aggacgtcga tcttctcccc
      361 ctccagctct cggatgatgg actggatgcg gatgcccttg agtcctacgc acgctcccac
      421 ggggtccacg tcctcccgct tggagaagac ggagatcttg gtgcggtatc cgggttcccg
      481 gacgatcttg aagacctcga cgatgttgtc gtagatctcc ggcacctcga gctccaggat
      541 cttgcggacg aactccgcgt gggtgcggga caggacgatc tggggacccg tcttggtctt
      601 gttgacctct acgatgtagg ccttgatccg gtcgttggga tggtaggtct cccgggggga
      661 ctggtacttc ttgggaagca ccccttccac cctgcccagg tccacgtaga tgttgccgtt
      721 ccggtcgcgc tggtagtatc cgatgatgat ctcgccgacc ttggacttgt actccgcgta
      781 gagggtgtcc cgggagatct cccggaggtt ctgccgcgtg gtctgcttgg ccagcatgac
      841 ggattggagg tcgaactccc ggggatccac gggaagctcc aggatgtccc cgatttcggc
      901 ttcctcgctg taggcccggg cctcgtccag ggtgatctcc aggaccggat cgtcgagttc
      961 ctcgacgatg gtcttcttcg cgaagatgtc cactccctcg tagttttccc ggaaacgcac
     1021 gaccgcgttt tccgaggtac cgtagcgttt cttgtacgcc gcgaggagag agtcttcgat
     1081 cgtcttaatc acgagatcct cggatatgcc cttctcgtag attagctggc gtatcgcctc
     1141 ggccatttct gaagacatgg cgttaagctc cctcctgtgc gtaatcgagc cgcgcttttg
     1201 ctatggatgc gtattcaacg gtccgttcgc cccaggctcc ccggatcgtc atcgcggacg
     1261 ggcctgcctg ggctatgcgg ccggcgagcc actcgtcccc ctccttgagg aggatccgaa
     1321 tgccccggcc ggtgaatatg gcgtactccc gatcggaacg gatccatcga tccaccccgg
     1381 gggaggcgac ctcaaggtgg aagtcctgct cggaaagcag gacctgcagc ctgggctgga
     1441 tgagcctgtg ggcccgggaa cactcgtcga tccccgtacc gccgggggcg tagattaccg
     1501 cccggacctg cacggagccg cgatgacggc tgacggacag gtccacgagg cccagtccgg
     1561 cgcccgagag gagttcctcc agatccttcc tgtatctgtc gctttccacc atacctacaa
     1621 aaaaagagtc gtgggaacga ctcttcttat ccctaagaat aagctctcta cggaaggtat
     1681 agtataagaa gccgctcccc ttcgtcaagc cgcggggacg gcaccgtccc cgcgggcgcc
     1741 gcgggtccgg ggaccccgac ggctcggccg atccgggggg ccggaaagga gtcaatccgc
     1801 cgccaggacc agggcatcca cccgacgtgt ccccgctgcc ttgagcaccc gggcgcactc
     1861 ggagagggtc gcgcccgtgg tcagcacgtc gtccaggagc acgacccgcg ccggcgcctt
     1921 tgctcccttt cggacgcgga tctttcccgt caggttggac atccgggcgg agtaatccag
     1981 ggttttctgg gaggctcctt ccgccctctc caggcagcgc atcacgggca ccccccttcg
     2041 ttccaggctc cgggccaggt cctcgacctg gtcccatccg gttttgcgga tcttgccctt
     2101 ccggaacggg acgggaacca ggacggtccc cggataccct tcccgaaggc ggtcgtatac
     2161 ccgggaggcc aggaagcggg ccaggggccg cactccccgt atcttgtagg agatgaggag
     2221 atccttgatc cgccccgcat actcccagag ggggtacgcc gaatcgaagg cgaagctgcg
     2281 gtcccggcag cgcatgcaga gcccccgctc ggaaacgagg ggcttgccgc atatcggaca
     2341 tcgttctccc cggatccggg ggaggtcctc ctcgcattcg ccgcacaggg gatacgcccg
     2401 gtcggcttcc tcgaggagcc cgcggccgca aaggacacag caccggggaa acaggatctc
     2461 cagagccctg ctcaaaaggg aaggcgaaca gtccatgacg gaataccgtc cgccgagccc
     2521 tcccccgctt gcgaggctat agcagtttct tgagccgggc cagacgggag agggcctcca
     2581 ggggggtgat gcggtcggga tccagggagc ggagctcctc cagaaccagt tcctcgttgg
     2641 aaaagagggc ggcctgggcg ggttgcctcg gggacgccac ggcggcgggc aggtcccgct
     2701 ccttccgggc caggttttcc cgcacctggc gggcccgggc caggaccggg gcgggtatcc
     2761 ccgcgagacc cgccacgtga agaccgtagg aacccgcggc gggcccctcg accacccttt
     2821 tcaggaatac cacctccccc gcgctctccc ggaccgcgag ggagcggttg accagcttgg
     2881 gatgctccag ggccgtcagc tcgtggtaat gggtggcgaa gagggtccgg catcccacgg
     2941 cgtccagcag gtgttcggcg gccgcccagg cgatggccaa cccgtccagg gtcccggtcc
     3001 ccctccccac ctcgtccatg atgaccaggc tgcggggtcc ggcattgtta aggatgtagg
     3061 cggtctcgtg catctccacc aggaaggtgc tctctccccg ggccaggttg tcctgggcgc
     3121 cgacccggca gaatatccgg tccaccaccc cgatcctggc ctcaccggcg ggcacgaagg
     3181 aaccggcttg ggccagcagg acgatcaggg cggtctgccg gaggtaggtg gacttgccgg
     3241 ccatgttggg acccgtgatc agggcgaagg agacctcccc ggagtccagc cgcacgtcgt
     3301 tgggcacgaa gtccccgccc gcccgatggg cttccaccac gggatggcgg ccttccagaa
     3361 tgcccaaggt cccggtatcg tcgacctcgg gacgggtgta gccccgggct gtggcggtgt
     3421 acgcgaagga ggccaggcag tcgacccggg cgcaggctcg ggcggcttcc agcaaccgcg
     3481 gcacggcggc cttgacctcg gtccgcacct ccaggaacct ctcccgctcc cgttccacga
     3541 tccgctcctg ggccccgttg atgcgggatt ccagctctgc caggcggtcc gtggtgtacc
     3601 gctccccggt ggagagggac tgccggcgta tgaagtggga gggcacctgg ggaagggcgc
     3661 ccttggtgac ttccaggtaa tagcccagga tgcggttgta ccggatcctg aggttctgga
     3721 tgcccgtggc ggtccgttcc tcctccaggt acgactccag gacggatcgg gaggaatccc
     3781 ggaggtcccg cagggcgtcc agctccggat cgaagcccgg ccgtatcagg ttcccctcgt
     3841 tcaacaggac cgagggatcc tccaggatgg cgcggccgac caggtccgac acggaccgga
     3901 gctcctcctc gacctcgggt cccaagtcca gtccgagctc cggcgcggcc ccggtctcgg
     3961 gctccaggag ggcggcggct tcccggatcg tgtccaggga acccttgatt ccgaggagat
     4021 ccttggcatg ggccttgtcc atggccaccc gggaagcgag gcgttccagg tccatacagt
     4081 tgcccagaca ggaccggagc cgctccaggg tgcgctggtt ccgatacagc cgttccacgg
     4141 cgtccagccg ggaccggatc gcggcaggat ccctcagggg ccggagaagc cagtgcttga
     4201 ggagccgggc ccccatggcg gtccgggtgg cgtccaggac gtccaggagg gtgaacgccc
     4261 ggccgccgtc ctggaggttc cgatcgatct ccaggttcct ctgggcggat tcgtcgatgt
     4321 ccgcgaaagc ctcctccctc cggggccgca gaccctccag gtggggaacg gcaccccgga
     4381 aggcgtcctc caggtaccgt accaggacgc ccgccggcgc cagggccgga tcgtcgtcgg
     4441 aaaaaccgaa gcccttgagg gaagccgtcc cgaaccgctt tgtcaggatc tccgacgcct
     4501 gggcctggtc gtagctccag tccgggaaac ggttcaggat gagcccctcc ctttcctgga
     4561 gtatctgggc gatatccggg tcgtccagca gggtctgttg gaccagggct tcccgagggg
     4621 ccaggcggta cagctccttg cggagcctgg ccggggcgtc ctgcttcgcg gagaaggcgc
     4681 agaattcccc ggtggatacg tccacgtaag cgatccccag tacgtcccga acgcggccga
     4741 tggacacgag gtagttgttg gacctgctgt ccaggtagtc ttcctccacg gtggtgcccg
     4801 gggtgatgat ctctaccacc tcccgatcca tgagcccccg ggaaacccct gcgaccgcgg
     4861 tctgttcgca gatggcgatc ttcttgcccg ccttgaggag acgggagatg taggaatggg
     4921 cggcgtggta cgggatcccg cacatgggcg cgtcctgacg cttggtcagg gtcaggttga
     4981 gcagggagct gacttccagg gcgtcccgtt cgaacatctc gtagaaatct ccgaggcgga
     5041 agaacaggat ctcgtcccgg tgg
//
LOCUS       DAOTEI010000219         5033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_12037_length_5033_cov_4.883487, whole genome shotgun sequence.
ACCESSION   DAOTEI010000219 DAOTEI010000000
VERSION     DAOTEI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12037_length_5033_cov_4.883487"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="P5313_15360"
     CDS             complement(<1..307)
                     /locus_tag="P5313_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81792.1"
                     /translation="MTFSEKIKNLVNTGLDTSRDILSKAGGKAQQWGEMGVLKVEIVQ
                     LRGQAEKMTARLGAEVYETLVEKGQKTVSRDTPSIRDILGKIEELGKKEDALVGVPSG
                     "
     gene            565..1041
                     /gene="nrdR"
                     /locus_tag="P5313_15365"
     CDS             565..1041
                     /gene="nrdR"
                     /locus_tag="P5313_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRY81793.1"
                     /translation="MRCPHCGSLDDRVIDSRTLANGESIRRRRECLACEFRFTSYERI
                     EEKPLMVVKRDGRREPFERAKLERGVQRAVEKRPISQLSIENIVNEIEDEAAVRGKGG
                     NEIASSVLGDMVLHRLYDIDKVAYVRFMAVYRSFESVDEFLKVVEQIESERKGPAL"
     gene            1213..3315
                     /locus_tag="P5313_15370"
     CDS             1213..3315
                     /locus_tag="P5313_15370"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HRY81794.1"
                     /translation="MSDQHVFPEWRSFLGSPAGEGGTAFVKSVVKRSGQVEKYDRAKI
                     EEAIAKAFRAVSGVAEKDTVGRLADAVEDRLRLILAGRHRNSIPAIEEIQDVVETVLV
                     EAREVEVAKAYILYRARHEVLRDTKTLMLDINSTMDGYLAQADWRVKENANVNYSLGG
                     LILHNSGSITANYWLKNIYPEEIARAHRSADFHIHDLSMFSGYCAGWSLRALIKEGFG
                     GVPDKISSTPAKHLSTLVQQMVNFLGVLQNEWAGAQALSSFDTYLAPFVKADSLPESE
                     VKQCIQSFLFGVNTPSRWGSQAPFTNVTLDWTVPADLAKLPAVVGGTEQPFTYGDCQA
                     EMDLINKVFIDLLLEGDAEGRGFQYPIPTYNVTRDFNWDTPNARRLFEMTAKYGTPYF
                     QNFINSDLDPGDVRSMCCRLQLDKRELRKRGGGLFGSDELTGSIGVVTLNLPRIGYLA
                     DSRTDFFRRLDRLLDLAAQSLRIKRKVCSRLLEAGLFPYTRRYLRSFDNHFSTIGLVG
                     MHECCLNFLGKGIETPDGKAFSLEVLGYMRKRLQDFQEETGELFNLEATPAESTSYRL
                     AKHDKKQYPEIVTSGREEPYYTNSSQLPAEFTEDLFDALDHQEELQTQYTGGTVFHIF
                     LGEAVEEWEAVRDLVKAVAGGYRIPYFTISPTFSVCPVHGYLSGKHFACPRCKEEKEA
                     RLREEIRALERELAEGRE"
     gene            3450..4019
                     /gene="nrdD"
                     /locus_tag="P5313_15375"
     CDS             3450..4019
                     /gene="nrdD"
                     /locus_tag="P5313_15375"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRY81795.1"
                     /translation="MDTATIEKRLAGLKADLASVQGTPTEVYSRIVGYYRSVKNWNAG
                     KREEFSHRATYRLPSGIPSALGDAPQPPAPVSGHALLWEAPAEHPDLFTGEQAPTLSD
                     VMGPASYILFTRRSCPNCPAVRDYLEGTGLLGREADVDSPQGLELARAYGVLSCPTAV
                     LRDLEDREVYRAYSVTELRAFLETVPETA"
     gene            4025..4744
                     /locus_tag="P5313_15380"
     CDS             4025..4744
                     /locus_tag="P5313_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRY81796.1"
                     /translation="MEAPAAERTAAWGVAVQKTSLIDYPGRVSAVLFLAGCNFRCPYC
                     HNPDLVLPPFPEDLSTAGDALAFLDTRRRVLSGVVLSGGEPLLREDLPLLARELRDRG
                     FKVKLDTNGSFPDRIEGVGADFVALDLKTTPDRYDRVAPGVPDAGERVLESLRVVRSL
                     GVPYELRITCAPGIAETGEIRSLAELLEPEDSVVLQGFRPLRVLDPLWETLPATPERL
                     LEDYAGLLRTRAARVRVRSST"
     gene            4784..>5033
                     /locus_tag="P5313_15385"
     CDS             4784..>5033
                     /locus_tag="P5313_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY81797.1"
                     /translation="MDEPFHPSFHPLIPSWFRETYGEPTAVQAAAWPRIAAGEHVLAL
                     APTGSGKTLAAFLDAISRLVRGEYEPGRLSVLYVSPLKA"
BASE COUNT          868 a         1719 c         1623 g          823 t
ORIGIN      
        1 acccggaggg cacgccaaca agtgcatcct ctttctttcc cagttcctcg atcttcccca
       61 ggatatcccg gatggagggg gtgtcccggc tgaccgtctt ctggcccttc tccaccaggg
      121 tctcgtagac ctcggcgccc agacgggcgg tcatcttctc cgcctgtccc cggagttgga
      181 cgatctcgac cttgagcacc cccatctcgc cccactgctg ggccttgccc cccgccttgg
      241 aaaggatgtc ccgggacgtg tccaggcccg tgttgaccag gttcttgatc ttctcggaaa
      301 acgtcatgat accccctacg gccgatcgaa tatgaaattc cgtcctttat aacatagcct
      361 ctttagcggc ccgggggaag cttttcccgc acggacgaaa aaaaaccgca aagccttgac
      421 atccgtccgt tccgccacta tatcctgtag tcgatcgtga tagatactac tacttgttgt
      481 atagtctgat tgcaaaaagg ataccaaggg gatgtcggat cggggatctg ggacgaaaaa
      541 aaaccgaaaa agccggagcg ccgcgtgagg tgtccccact gcggcagcct ggacgaccgg
      601 gtcatcgatt ccaggaccct ggccaacggg gagagtatcc gcaggcgccg ggagtgcctg
      661 gcctgcgagt tccggttcac cagctacgag cggatcgagg aaaaacccct catggtcgtg
      721 aagcgggacg gccgccggga gcccttcgag cgggccaagc tcgagcgggg cgtccagagg
      781 gccgtggaaa agcgtcccat atcccagctc tcgatcgaga atatcgtgaa cgagatcgag
      841 gacgaggccg ccgtgcgggg caagggcggc aacgagatcg cgagctccgt cctgggggac
      901 atggtcctgc accggctcta cgacatcgac aaggtggcgt acgtccggtt catggcggtg
      961 tatcgaagtt tcgagagcgt ggacgagttt ctgaaggtgg tcgagcagat cgagtcggag
     1021 agaaaagggc ctgccctgtg aaggtcgtga aggtatcgtg aactagggaa cgggccgcgc
     1081 gccggcttgg gagacggcgc gcggccgggc ggcggcggag gttccgaggc ccggggtcgc
     1141 ggccccgggc cggagagccg cccggggcgg ccggtatccg gccgcccccg ttcccggtcc
     1201 tgggagggga ctatgtcgga ccagcatgtc tttcccgaat ggaggagctt tctgggctcc
     1261 ccggccgggg agggcggaac cgcttttgtc aaatccgtgg tcaaacgctc cgggcaggtc
     1321 gagaagtacg accgggccaa gatcgaggag gccatagcca aggccttccg ggccgtttcc
     1381 ggtgtcgcgg aaaaggacac cgtgggccgc ctggccgacg ccgtcgagga ccggctccgc
     1441 ctgatcctgg cgggcagaca ccgcaactcc atccccgcca tcgaggagat ccaggacgtg
     1501 gtggagacgg tcctggtgga ggcccgggag gtggaggtcg ccaaggccta catcctctac
     1561 cgggcccgtc acgaggtcct ccgggacacc aaaaccctca tgctggacat caactccacc
     1621 atggacgggt acctggccca ggcggactgg cgggtcaagg agaacgccaa cgtcaactac
     1681 tccctggggg gactgatcct ccacaactcg ggctccatca ccgccaacta ctggctcaag
     1741 aacatctacc ccgaggagat agcccgggcc caccggtcgg cggacttcca catccacgac
     1801 ctgtccatgt tctccgggta ctgcgccggc tggtccctcc gcgccctgat caaggagggc
     1861 ttcggcgggg tgcccgacaa gatctcctcc acccccgcca agcacctgtc caccctggtc
     1921 cagcagatgg tgaacttcct gggggtcctc cagaacgagt gggcgggggc ccaggccctt
     1981 tcctccttcg acacctacct ggcgcccttc gtcaaggccg actccctgcc ggagtcggag
     2041 gtcaagcagt gcatccagag cttcctgttc ggggtgaaca cccccagccg ctggggcagc
     2101 caggcgccct tcacgaacgt caccctggac tggacggtac ccgcggacct ggccaagctc
     2161 cccgccgtgg tcggcgggac cgaacagccc ttcacctacg gggactgcca ggcggagatg
     2221 gacctgatca acaaggtctt catcgacctt ctgctggagg gcgacgccga gggccggggc
     2281 ttccagtacc ccatcccgac ctacaacgtg acccgggact tcaactggga cacccccaac
     2341 gcccggcgcc tcttcgagat gaccgccaag tacggcaccc cctacttcca gaacttcatc
     2401 aactccgacc tggatcccgg ggacgttagg tccatgtgct gccgcctcca gctggacaag
     2461 cgggagctcc gcaagcgggg agggggcctg ttcgggagcg acgagctcac gggatccatc
     2521 ggggtggtca ccctgaacct cccccgcatc ggctacctgg ccgattcccg gacggacttc
     2581 ttccgccgtc tggaccggct cctggacctg gccgcccaaa gcctccggat caagcggaag
     2641 gtctgctccc ggctcctgga ggcggggctc ttcccctaca cccggcgcta ccttcggtcc
     2701 ttcgacaacc acttctccac catcggcctc gtggggatgc atgagtgctg cctgaacttc
     2761 ctgggaaagg ggatcgagac cccggacggc aaggccttct ccctggaggt cctgggctac
     2821 atgcgcaagc ggctccagga cttccaggag gagaccgggg agctcttcaa cctggaggcg
     2881 acccccgcgg agagcacctc ctaccgcctg gccaagcacg acaagaagca gtaccccgag
     2941 atcgtcacct ccggccggga ggaaccctac tacacgaact cctcccagct gcccgcggag
     3001 ttcacggagg acctcttcga cgccctggac caccaggagg agctccagac ccagtacacc
     3061 ggcggtacag tcttccacat tttcctgggt gaggcggtgg aggaatggga ggcggtccgg
     3121 gacctggtga aggccgtagc gggcggttac cgcattccgt acttcacgat ctccccgacc
     3181 ttctcggtct gccccgtgca cggctacctc tcgggcaagc acttcgcctg cccccggtgc
     3241 aaggaggaga aggaggcccg gctccgggag gagatccggg cgctggagag ggagttggcg
     3301 gaaggaagag aataagtgaa tgggtgaatg ggtgaatggg tgaatggagc ggctgtagcc
     3361 gcttgaggtc ctgaacgcgt cgagaatcga attcaggaat cacgtattca cgattcaccc
     3421 cttcaccgat ttacctcaca ggagggtaca tggatacggc aacgatcgaa aaaaggctgg
     3481 ccggcctgaa ggcggacctc gcttccgtcc agggcacccc cacggaggtg tacagccgga
     3541 tcgtgggata ctatcgctcc gtcaaaaatt ggaacgcggg caagcgggag gagttttccc
     3601 atcgggcgac ctaccgcctg ccctccggga tcccctcggc cctgggggac gcccctcagc
     3661 cgcccgcgcc ggtctccggc cacgccctcc tctgggaagc cccggcggag catcccgacc
     3721 tcttcaccgg ggaacaggcg ccgaccctga gcgacgtcat ggggccggcc tcgtacatcc
     3781 tgttcacccg ccgttcctgt cccaactgcc cggccgtccg ggactacctg gagggcacgg
     3841 ggctgctggg gagggaggcg gacgtcgatt cgccccaggg cctggaactg gcccgggcct
     3901 acggggtgct ctcctgtccg accgcggtgc tccgggacct cgaggaccgg gaggtctacc
     3961 gggcgtacag cgtcacggag ctccgtgcct tcctggagac ggtccccgag acggcctagg
     4021 caccgtggaa gccccggcgg cggagcggac ggcggcctgg ggggtagccg tccagaagac
     4081 cagcctcatc gactatcccg gccgggtctc cgccgtgctc ttcctggcgg gctgcaactt
     4141 ccgctgtccc tactgccaca atcccgacct ggtccttccc cccttcccgg aggacctgtc
     4201 caccgccggg gatgccctgg ccttcctgga cacccggagg agggtcctgt ccggtgtggt
     4261 cctctccggg ggggagcccc tcctgcggga ggacctgccc ctcctggccc gggagctgcg
     4321 ggaccggggc ttcaaggtca agctggacac caacggctcg ttcccggacc ggatcgaggg
     4381 ggtgggggcg gacttcgtcg ccctggacct gaagacgacc ccggaccgct acgaccgtgt
     4441 cgcgccgggg gttccggacg cgggggaacg ggtcctggaa tccctccggg tcgtgcgctc
     4501 cctcggggtg ccctacgagc tccgcatcac ctgcgccccc ggcatcgccg agaccgggga
     4561 gatccggagc ctcgccgaac tcctggagcc cgaggattcc gtggtcctcc agggcttccg
     4621 tccactgcgg gtcctggatc ccctctggga gaccctgccc gccacgccgg aacgcctcct
     4681 ggaggactac gccgggctgc tgcggacccg ggccgcccgg gtccgcgtcc ggtcctcgac
     4741 ctagaacttg aagccccgcg gccgccgggg taccatgacc ggcatggacg aacccttcca
     4801 cccgagcttc caccctctca tcccttcctg gttccgggaa acctacgggg agcccacggc
     4861 cgtccaggcg gcggcctggc cccggatcgc cgcgggggag cacgtcctgg ccctggcccc
     4921 cacggggagc ggcaagaccc tggccgcctt cctggacgcg atctcccggc tggtccgggg
     4981 ggagtacgag ccgggtcggc tctcggtgct ctacgtctcc cccctgaagg ccc
//
LOCUS       DAOTEI010000220         5006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_12124_length_5006_cov_4.276712, whole genome shotgun sequence.
ACCESSION   DAOTEI010000220 DAOTEI010000000
VERSION     DAOTEI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12124_length_5006_cov_4.276712"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="P5313_15390"
     CDS             complement(<1..319)
                     /locus_tag="P5313_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRY81798.1"
                     /translation="MKHSVAYDVNGDKLILETGHMAKQANGAVFAKFGGSAVIATACC
                     ASSATEGLDFVPLTVEYNEKYYAAGKIPGGFIKRETRPKDKEILVSRLIDRPMRPLFD
                     KAFG"
     gene            complement(397..666)
                     /gene="rpsO"
                     /locus_tag="P5313_15395"
     CDS             complement(397..666)
                     /gene="rpsO"
                     /locus_tag="P5313_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRY81799.1"
                     /translation="MAMTKEDKARVVLEYGAGEKNTGATEVQIALITERIKYLTEHFK
                     ANKKDTNSRRGLLQLVGQRRRLLKYLQAENLQSYRSLIEKLGIRK"
     gene            complement(692..1477)
                     /locus_tag="P5313_15400"
     CDS             complement(692..1477)
                     /locus_tag="P5313_15400"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HRY81800.1"
                     /translation="MRILDWGSWTEGAGGSARPSAVTIGVFDGVHAGHRVLIGRILAR
                     AENLEPTVVTFRQNPKELLHPETFHGAVLTLRQKLESLEESGIGTCVLIDFSGNFSKL
                     TGTEFLSALADKGQARYLAVGSDFRCGHRLSTDAESVRRIGSSLNVDTEIVDPVLYHG
                     RAVSSSRIRRAVLEGRMPDAFAMLDRPYVLDLREFVPSKEGDALRYDGIRQVLPPPGI
                     YPAEASTADGWRKTGLGVLSGESALVEAAGGDPPGFLRFSIHG"
     gene            complement(1474..2367)
                     /gene="truB"
                     /locus_tag="P5313_15405"
     CDS             complement(1474..2367)
                     /gene="truB"
                     /locus_tag="P5313_15405"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRY81801.1"
                     /translation="MRKRSSDSNGFLVLDKPSGITSFEALGSVKRAFDTGRVGHTGTL
                     DRFARGVLVALVGRYARLSEYFTASDKVYLAGIRFGTETDTLDPEGTIIARGPLPDRA
                     ALEAVLPTFRGDILQAPPAFSAVHIEGRRAYERALAGEDVRPEPRPVTILGLDLLDYS
                     DGLARMRVRCSKGTYIRSLARDIAEACGTRAHLETLVREASGPFRIEDGIPPEAADSS
                     SLRVLDPELCETLGLPPVRLAPGQEAFFLRGGPLTKGRFAKPPEGVGFRGVFSAEGTF
                     LGVLEIRDGTYSYRVVLGTSA"
     gene            complement(2364..2711)
                     /gene="rbfA"
                     /locus_tag="P5313_15410"
     CDS             complement(2364..2711)
                     /gene="rbfA"
                     /locus_tag="P5313_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRY81802.1"
                     /translation="MDDIRRKRVEEELRSEIASLILSGEIKDPRINAFLSITRVEVSR
                     DASAARVHVSTFEEGEALTRGVEGLNNAAGFIQAAVGRKIRLRLTPKLKFLADDGIRK
                     GFEMGEKIKDLFS"
     gene            complement(2716..>5006)
                     /gene="infB"
                     /locus_tag="P5313_15415"
     CDS             complement(2716..>5006)
                     /gene="infB"
                     /locus_tag="P5313_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRY81803.1"
                     /translation="YQGQSGGYQGRPGGYQGRPGGYQGGPGQGSGGYQGQSGGYQGRP
                     GGYQGRPGGYQGGPGQGSGGYQGQSGGYQGRPGGYQGRPGGYQGGPGQGTGGYQGRPG
                     GYQGRPGGYQGGPGRGPGGPGRGPGAGTSPTIDKRPQGPKRFIKSKKPTYNKKDREEE
                     FSQKLNLSKKKQGAVANPIPTKIDILETISVSELARKMNLKPSELIQKLMKLGFMATI
                     NQQIDSDTATILAGEYGCEVRVVSLYDETVIEKQKDNPEDLVHRPPIVTVMGHVDHGK
                     TKLLDAIRKTDVVAGEFGGITQHIGAYQVDTGKGKITFLDTPGHEAFTMMRARGSQVT
                     DIVVLVVAATEGVMPQTLEALDHAKAAGVPIIVAVNKIDLPDANPDRVKTQLSDKGLV
                     AEDWGGTTMFCPISALKKQGISELLDAILLQAEVLELTANYDCRAEGKVLESKIDHGR
                     GIVSTVIIERGTLRVGDSFVAGIYPGKVRALYDDHGVKVDEAPPSFPVEVIGFEGMPN
                     AGDPFEAVEDEKFARQISDKRQELKRYEEAKTVKKVTLDNLYDTIHEGTVQELKVVIK
                     GDVHGSVEALKSSLEKLSTKEVRLNVIHASAGAIHESDIMLASASNALIIGFNVRPTP
                     KAKLLADQEKVDIRKYNVIYKAVEEIEKAMEGMLAPEYKEQDIAQAEVRETFHVPKIG
                     VVAGCMVTEGTVKRNAGVRVIREGIEIFSGKITSLKRFKDDAKEVAAGFECGIGVENC
                     NDIKVGDILEIFEQVQVTRKLS"
BASE COUNT          770 a         1641 c         1612 g          983 t
ORIGIN      
        1 ggccgaaggc cttgtcgaag agggggcgca tgggccggtc gatgaggcgg gagaccagga
       61 tctccttgtc cttcggacgg gtctctcgct tgatgaagcc gccgggaatc ttgccggcgg
      121 cgtagtactt ctcgttgtat tccacggtga gggggacgaa gtccaggccc tcggtggcgg
      181 aggaggcgca gcaggcggtg gcgatcaccg cggagccgcc gaatttcgcg aacacggcgc
      241 cgttggcctg cttggccatg tggccggtct cgaggatcag tttgtcgccg ttcacatcgt
      301 acgcgacgga atgtttcatc gtaggttctt tctccattgt ccggtaaata aaagggcccg
      361 aacaaccgtt cgttcgggcc ttcacgtcag gtccgcctac ttgcggatac ccagtttttc
      421 gatgagggac cgataactct gtaggttctc ggcctgcagg tatttcagga ggcggcggcg
      481 ctggccgacc agctgcaaga ggccccgccg ggaattggtg tccttcttgt tcgccttgaa
      541 gtgctccgtt agatacttga tccgctccgt gatgagggcg atctgcacct ccgtggcccc
      601 ggtattcttc tcgccggcgc cgtactcgag gaccacgcga gccttgtcct ctttggtcat
      661 tgccatacct atactccttg cctctgcatt gctagccgtg tatcgaaaat cgcaggaacc
      721 cgggaggatc cccccccgcc gcctcgacga gcgcgctttc tccggaaagc acgcccaggc
      781 cggtttttct ccaaccatcc gccgtcgacg cttccgccgg gtatatgccg ggagggggca
      841 gaacctgccg gatcccgtcg tacctcaggg cgtcgccctc cttcgaagga acgaactccc
      901 gaagatccag gacatagggc cggtcgagca tggcgaaggc gtccggcatc ctcccctcca
      961 gtacggcccg ccgaatccgg ctggaactca cggcacggcc gtggtacagg acgggatcca
     1021 cgatctcggt atccacattc aacgaagagc cgatgcgtcg aacgctctcg gcgtccgtgg
     1081 aaagccggtg cccgcagcgg aaatccgatc ccaccgccag gtaccgggct tgccccttgt
     1141 ccgcgagggc gcttagaaac tcggtccccg tcagtttact gaaatttccg gaaaagtcaa
     1201 tcaggacgca agtccctatt cctgactcct caaggctttc cagcttctgc cggagggtga
     1261 gtacggcccc gtggaaggtc tcgggatgca ggagttcctt gggattctgc cggaaggtga
     1321 cgaccgtggg ctccaggttc tccgcccggg ccaggatacg tccgatcagc acccggtggc
     1381 ccgcgtggac tccgtcgaag acaccgatgg tgaccgcgga aggccttgcg gaccctcccg
     1441 ctccttcagt ccaggaaccc cagtccagga ttctcatgcc gatgtcccga ggaccacccg
     1501 gtaggaatac gtaccgtccc ggatttccag gacgcctagg aaggtcccct ccgcggagaa
     1561 gactccccgg aatcccacac cctccggtgg tttggcgaag cgccccttgg tgagaggacc
     1621 gccgcgtagg aagaaggcct cctgccccgg agcgagccgt accggcggga gtcccagggt
     1681 ttcgcagagt tccggatcca ggacccgcag cgacgaggag tccgctgcct ccggcggtat
     1741 cccgtcttcg atccggaagg ggccggaagc ttcccggacc agggtctcca ggtgtgcccg
     1801 tgtcccgcag gcctccgcga tgtcccgggc tagggaccgg atgtaggtgc ccttggaaca
     1861 ccggacccgc atacgggcca gcccgtccga atagtccagg agatccaggc cgaggatggt
     1921 caccgggcgg ggctcgggac ggacgtcctc gcccgccagg gccctctcgt aggcgcgcct
     1981 gccctcgatg tggacggcgg aaaaggcggg aggggcctgg aggatgtccc cccggaaggt
     2041 aggaaggacg gcttccaggg cggcgcggtc cggcaggggc ccccgggcga tgatggttcc
     2101 ctccgggtcc agggtgtccg tttccgtccc gaagcggatt cccgccaggt agaccttgtc
     2161 ggaagccgtg aagtattcgg agaggcgggc gtatcggccc accagggcga ccaggacgcc
     2221 ccgggcgaag cggtccaagg ttcccgtgtg cccgacccgg ccggtgtcga aggcgcgctt
     2281 gaccgacccc agggcctcga aggaggtgat cccggagggc ttgtccagta ccaggaagcc
     2341 gttggaatcc gaggagcgtt tcctcacgaa aacaggtcct tgatcttctc gcccatctcg
     2401 aagcccttgc ggatgccatc gtcggccagg aacttgagct tgggggtcag ccggaggcgt
     2461 atcttccggc cgaccgccgc ctggatgaat ccggcggcgt tgttcaggcc ctccaccccc
     2521 cgggtcaggg cctcaccctc ctcgaaggtg gagacgtgga cccgggcggc gctggcgtcc
     2581 cgggacactt ccacccgggt gatcgaaagg aaggcgttta ttcggggatc cttgatctcc
     2641 ccggagagga tcagggaggc gatctccgac cggagctcct cctccaccct ctttctccgt
     2701 atgtcgtcca tagcctcagc tcaacttgcg cgtaacctgc acctgctcga agatctcgag
     2761 gatgtcgccc accttgatgt cgttgcagtt ctccacgccg atgccgcact cgaagcccgc
     2821 ggccacttcc ttggcgtcgt ccttgaagcg cttgagggag gtgatctttc cgctgaagat
     2881 ctcgatgccc tcccggatga cgcgcacgcc ggcgttccgc ttgaccgtgc cttcggtgac
     2941 catgcagccc gccaccacgc cgatcttcgg gacgtggaag gtttcccgga cctccgcctg
     3001 ggcgatgtcc tgctccttgt actccggagc cagcatgccc tccatggcct tctcgatctc
     3061 ctcgacggct ttgtagatga cgttgtactt ccggatgtcc accttctcct ggtccgcaag
     3121 gagcttggcc ttgggggtcg ggcggacgtt gaagccgatg atcagggcgt tggaggcgct
     3181 ggccagcatg atgtcgctct cgtggatggc ccccgcgctg gcgtggatca cgttgaggcg
     3241 cacttccttg gtggacagct tctccaggga ggacttcagg gcttccacgg aaccgtggac
     3301 gtcgcccttg atgacaacct tgagctcctg gaccgtgcct tcgtggatgg tgtcgtagag
     3361 gttgtccagg gtgaccttct tgacggtctt ggcctcctcg taccgcttga gctcctggcg
     3421 cttgtcggag atctggcggg cgaacttctc gtcctccacc gcctcgaagg gatccccggc
     3481 gttgggcatg ccctcgaacc cgatgacctc caccgggaag gagggaggag cctcgtccac
     3541 cttgaccccg tggtcgtcgt acagggcccg gacctttccg gggtagatgc ccgccacgaa
     3601 ggagtcgccg acccgcaggg ttccgcgctc gatgatgacc gtggaaacga tgccccgccc
     3661 gtggtcgatc ttggactcca ggaccttgcc ttccgcgcgg caatcgtagt tcgcggtcag
     3721 ttcaagcacc tcggcctgca ggaggatggc gtccagcagt tccgaaatgc cctgcttctt
     3781 gagggcgctg atcgggcaga acatggtggt cccgccccag tcctcggcca ccaggccttt
     3841 gtcggaaagc tgggtcttca cgcggtcggg attggcgtcg ggcaggtcga tcttgttgac
     3901 cgccacgatg atgggcaccc cggccgcctt ggcatgatcc agggcttcca gggtctgggg
     3961 catgacgcct tccgtggccg ccacgaccag gaccacgatg tccgtgacct gggatccccg
     4021 ggcgcgcatc atggtgaagg cctcgtggcc cggggtgtcc aggaaggtga tctttccctt
     4081 gcccgtgtcc acctggtagg cgccgatgtg ctgggtgatg ccgccgaact ccccggctac
     4141 cacgtccgtc ttccggatgg cgtccaggag cttggtcttg ccgtggtcga cgtggcccat
     4201 gaccgtaacg atgggcggcc ggtggaccag atcctcggga ttgtccttct gcttctcgat
     4261 gacggtctcg tcgtagaggg agaccacccg gacctcgcaa ccgtactcgc cggccaggat
     4321 ggtcgcggta tccgagtcga tctgctggtt gatggtggcc atgaatccca gcttcatgag
     4381 cttctggatg agctcgctgg gcttcaggtt catcttccgt gcgagttccg aaacggagat
     4441 ggtctcgagg atgtctatct tggtcgggat ggggttcgcg acagctccct gcttcttctt
     4501 ggacaggttg agcttctggg aaaattcctc ttcccgatcc ttcttgttat aggtgggttt
     4561 cttggacttg atgaagcgct tgggaccctg gggcctcttg tcgatggtcg gggaagtgcc
     4621 ggcgccgggt ccgcgccccg ggcccccggg tccccgaccg ggaccgccct gatagcctcc
     4681 cggacggccc tgatagccgc ccggccggcc ctggtagccg ccggtgccct gtccgggacc
     4741 gccctggtac cctccggggc ggccctgata gcctcccggc cggccctggt agccgcccga
     4801 ctgtccctgg tagcctccgg atccctggcc gggaccgccc tggtacccgc cgggacggcc
     4861 ctgatagcct cccggccggc cctggtagcc gcccgactgt ccctggtagc ctccggatcc
     4921 ctggccggga ccgccctggt acccgccggg acggccctga tagcctcccg gccggccctg
     4981 gtagccgccc gactgtccct ggtagc
//
LOCUS       DAOTEI010000221         4944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_12338_length_4944_cov_5.516261, whole genome shotgun sequence.
ACCESSION   DAOTEI010000221 DAOTEI010000000
VERSION     DAOTEI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12338_length_4944_cov_5.516261"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="P5313_15420"
     CDS             complement(<1..705)
                     /locus_tag="P5313_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81804.1"
                     /translation="MSPVRRRTGCSGHGTESGGDPSGFPGLRQILQEFRFLGLLGLLG
                     LLRLRRLGAVPDFPGFGRLFPPCFLQISEYVLQGRREAQGGQVGGLDILRNIFPVRGR
                     TVKCQGLDLGQGRTFYGFGFFYRFGLFYGFGLFDCFGLFDRLGFFDRFGFFYGFGLFD
                     CFGHFDRLGLFDRLGFFDGLDFFDCFSFFYGFGLFDRLGLFDRLGFLDSLGLFDGFGL
                     FDRFGFLDGFDLFDRFG"
     gene            667..1827
                     /locus_tag="P5313_15425"
     CDS             667..1827
                     /locus_tag="P5313_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81805.1"
                     /translation="MAGASGSAADRAHQLSKEDEDIPVIPEDLGLELVDETELSEISE
                     YLDVEGTRLSAILAAESLQESTPILEFGEAEEAEAVEEAEAVEEIEEVEEAEAVEAVE
                     EAEAVEEAEAVDEAEAVEEVEEAEAVEEAEAVEEVEAVEEAEAVEEVEAVEEVEEVEA
                     VEEVEAVEEVDLSEFGIAEERSKIPSAELLSRIEAMEFPESQAQEPEDLLEEVEFEVF
                     LSTLDLSALEGWNDEEGFLDFDLAAIREDIREEELDDIPLVGDEIEILSDEDRERLEE
                     LPAFEAPDASAELEASVGALPAYDYLPFDRRVVFAFRAQEGELENLGDGDLPEAEPLE
                     EFTGEASGSSDREVIVLEDGIYTVNRDAVDPDGLKDPSLAALAEEVLKDGSR"
     gene            1910..2674
                     /locus_tag="P5313_15430"
     CDS             1910..2674
                     /locus_tag="P5313_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81806.1"
                     /translation="MEAPRTFGIYLARGSSGEILLLHPFRPGSIVFREPETIVLEGRY
                     ASEPGPGEGDFIRKSLHGELERGLRRDALDRGFYPRLLVSAAAFLILYLFLSIVVRDP
                     VPLVDELLAGGLGAAAAWFALERRSLSSDAFARRTATLRRTLDTALFRPSRAAAYLEE
                     VLQEAESLDLERLAEYPASAGGSGFPEEDIRELEELADALEARLPPSVAEAAKESLSG
                     GGDLFRLLRKKAAGRAPYLPMLLTYVRIKTLCGAGA"
     gene            2671..3102
                     /locus_tag="P5313_15435"
     CDS             2671..3102
                     /locus_tag="P5313_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81807.1"
                     /translation="MKIRDLAGRVIRGYARALRSLGEALVVSAGMAGIAFAVVFPLWW
                     LAVNRRQLYTALVAGAGLAAAGFLVFRRFRSRHRNDPRTARAGFRVALILVPALLAAG
                     VYGTGVLFFRSPALGIPAALVLLATAGVWAFGRGERFDRER"
     gene            3099..4028
                     /locus_tag="P5313_15440"
     CDS             3099..4028
                     /locus_tag="P5313_15440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY81808.1"
                     /translation="MRPVPRAPVRRLLALCLGLGVASSLPAQVSPYPRIHRFEASDLS
                     FRQLSDSIAQSRRAEMTGSPFPDLLFFSYVLAEDTDLIALAARMNLPYESLATANRIG
                     ESRTLRAGQAVVVPSVTGIFAALDPSNDLEYLIAASRTPGDRDARARIRGPAGTSEFR
                     FLPGARFTPAERAFFLGTGFRFPLPKGRLTSSFGLRASPITGEIQHHAGIDLAAPAGT
                     EVFAARAGTVSEVGVDPVYGIYVLISHEGGWETLYGHLSARRVELNTTVKSGMMIGNV
                     GSTGLSTGPHLHFEVRIRGASRDPVPLLPRLGQ"
     gene            4025..>4944
                     /locus_tag="P5313_15445"
     CDS             4025..>4944
                     /locus_tag="P5313_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81809.1"
                     /translation="MKRYRALTIPAALAVLLFLPIASAIPEGVRTVVAAVVAVSADKP
                     DGETVSLGYADAVAVTLGAGSPFLQGVEIDLRIPRPLQSAQGAFAWSLYKAVTPVPAV
                     DKVAYDGDRLLIQALPPRAGVVFQVPVSPRHSLRSGPYATVLPMLLGPGDFPILFKLS
                     ALTKGVTPEQEQARYQVRIRPLFTDEGALRLSILPPEGAEPGFTPSVYVDERKLDSWQ
                     DLVFLKKGMHTVQVAGEGVRDESRAVAVEAGKVFSLEIRLQGTRPVVVFEAPANALIF
                     LDDQPVDHAGGARIQVEPGERTVVCRIGDYT"
BASE COUNT          835 a         1619 c         1715 g          775 t
ORIGIN      
        1 gccgaagcgg tcgaagaggt cgaagccgtc gaggaagccg aagcggtcga agaggccgaa
       61 gccgtcgaag aggccgaggc tgtcgaggaa gccgaggcgg tcgaagaggc cgaggcggtc
      121 gaagaggccg aagccgtaga agaagctgaa gcagtcgaag aagtcgaggc cgtcgaagaa
      181 gccgaggcga tcgaagaggc cgaggcggtc gaagtggccg aagcagtcga agaggccgaa
      241 gccgtagaag aagccgaagc ggtcgaagaa gccgaggcga tcgaagaggc cgaagcagtc
      301 gaagaggccg aagccgtaga agaggccgaa gcggtagaag aagccgaagc cgtagaaggt
      361 ccgaccctgg ccgaggtcga gtccctggca tttgaccgtt cggcctcgaa ctgggaagat
      421 attccggagg atgtcgagac ctccgacctg cccgccctga gcctcgcgtc tcccctggag
      481 gacgtactcg gaaatttgga ggaagcaagg cgggaagagc cgtccgaagc cggggaaatc
      541 ggggaccgcg ccgagccgcc ggagccggag gaggccgagg agcccgagga gcccgaggaa
      601 gcggaattcc tggaggatct gccggaggcc ggggaacccg gagggatctc cgccggattc
      661 ggtaccgtgg ccggagcatc cggttcggcg gcggaccggg ctcatcagct ctccaaggag
      721 gacgaggaca tccccgtcat cccggaggac ctgggcctcg agctcgtgga cgagaccgaa
      781 ctttccgaga tcagcgagta cctggatgtc gagggaaccc ggctgtccgc gatcctggct
      841 gccgaaagtc tccaggaatc cacgccgatc ctggaattcg gcgaggccga ggaagccgag
      901 gccgtcgaag aagccgaggc cgtcgaagag atcgaagaag tcgaagaggc cgaggcggtc
      961 gaagcggtcg aagaggccga agctgtcgaa gaggccgaag ccgtagacga agccgaagcc
     1021 gtagaagaag tcgaagaggc cgaggcggtc gaagaggccg aagctgtcga agaggtcgaa
     1081 gccgtcgaag aggccgaagc cgtcgaagag gtcgaagccg tcgaagaggt cgaagaggtc
     1141 gaagccgtcg aagaggtcga agccgtcgaa gaggtcgacc tatccgaatt cggaattgcc
     1201 gaagaaagat cgaagatacc ctccgccgag ctcctctccc ggatcgaggc catggagttc
     1261 ccggaatccc aggcccagga accggaggat ctcctcgagg aggtcgagtt cgaggtcttc
     1321 ctcagtaccc tggatctttc cgccctggag ggatggaacg acgaggaagg gttcctggac
     1381 ttcgaccttg ccgcgattcg ggaggatatc cgggaggaag agctcgacga catccccctg
     1441 gtgggggacg agatcgaaat cctctccgac gaggatcggg agcgtctcga ggaactgccc
     1501 gccttcgagg ccccggacgc gtccgccgag ctggaggcct ccgtgggagc ccttcccgcc
     1561 tacgattatc tccccttcga ccggcgcgtg gtcttcgcct tccgggccca ggaaggggaa
     1621 ctggagaacc tcggagacgg ggacctgccc gaggcggaac ccctggagga gttcaccggg
     1681 gaggcttccg ggtcctccga tcgggaggtc atcgtccttg aggacggcat ctatacggtc
     1741 aaccgggacg cggtggatcc cgacgggttg aaggacccgt ccctggccgc cctggccgag
     1801 gaagtgctga aggacggatc gcggtgagcc ggatccggat accggtcccc ggatccccga
     1861 tcccccggaa gtcttgacac cgcggcctac gcctcgtagg ctcctgtctg tggaagcgcc
     1921 ccggacattc ggcatctatc tcgcccgcgg aagttccggg gagatccttc ttcttcaccc
     1981 gttccgaccg gggagcatcg tcttccggga gcccgagacg atcgtcctgg aaggccggta
     2041 cgcgtcggaa ccggggcccg gagaagggga cttcatccgg aaatccctgc acggcgagtt
     2101 ggaacggggt ctgcgaaggg atgccctcga cagggggttc tacccgcgcc tgctggtttc
     2161 cgccgccgcc ttcctcatcc tgtacctgtt cctgtccatc gtcgtccggg atcccgtacc
     2221 cctggtcgac gagctcctgg ccggcggcct cggagccgcc gcggcctggt tcgccctgga
     2281 acggcgatcc ctgtcctcgg acgcctttgc ccgccgcacc gcgaccctcc gcaggaccct
     2341 ggataccgcg cttttccggc caagccgggc ggccgcgtac ctggaggagg tcctccagga
     2401 agcggaatcc ctggatctgg agcgcctcgc ggaatatccg gcttccgcgg gcggaagcgg
     2461 atttccggag gaggatatcc gggaactgga agagctggcg gacgccctgg aagcccggct
     2521 gcccccgtcg gtggcggagg cggccaagga atccctgtcc ggcggggggg acctgttccg
     2581 cctgctccgg aagaaggcgg ccgggcgggc cccgtacctg ccgatgctgc ttacctacgt
     2641 gcggatcaag actctgtgcg gagcgggggc atgaagattc gcgacctggc cggaagggtg
     2701 atccgcggat acgcccgcgc acttcgctcc ctgggcgagg ccctcgtggt gtccgcgggg
     2761 atggcgggga tcgccttcgc cgtggtgttt cccctctggt ggctcgcggt caaccgcaga
     2821 cagctctata cggccctcgt ggccggggcg ggtctggccg cggcgggatt cctggtcttt
     2881 cgccgattcc gatcgcggca tcggaacgat ccccgtaccg cgagggcagg tttccgggtc
     2941 gccctcatcc tggtccccgc cctgctggcc gcgggcgtct acgggacggg agtgctcttc
     3001 ttccgatccc ccgccctggg aatcccggcc gccctggtcc tgctggccac agcgggagtc
     3061 tgggccttcg gccggggcga acggttcgac cgggaacgat gaggcccgtt ccccgcgccc
     3121 cggtccggcg cctgctcgcc ctgtgtctgg gattgggtgt cgcctcctcc ctccccgccc
     3181 aggtgtcccc gtatccccgc atccaccgat tcgaggcctc cgacctgtcc ttccgacaac
     3241 tctccgactc gatcgcccag agccgcaggg ccgagatgac gggctcgccc tttccggacc
     3301 tcctcttctt cagctacgtc ctcgcggagg acacggacct gatcgccctg gccgcccgga
     3361 tgaaccttcc ctatgagtct ttggcgaccg cgaaccggat cggcgaatcc cggaccctcc
     3421 gggcgggcca ggccgtcgtg gtcccctccg tcacggggat cttcgccgcc ctggatccgt
     3481 cgaacgacct ggaatacctg atcgccgcgt cccggacgcc cggggaccgg gatgcgaggg
     3541 cgaggatccg cggaccggcg gggacttcgg agttccgctt cctgccgggc gcccggttca
     3601 cccccgcgga gcgggcgttc ttcctgggca cgggattccg ctttcccctg cccaagggac
     3661 ggcttaccag ctcgttcgga ctccgggcca gcccgataac cggggagatc cagcatcacg
     3721 cggggatcga cctggccgcc cccgcgggaa cggaggtctt cgcggcccgg gccgggaccg
     3781 tctcggaggt cggcgtcgat ccggtgtacg gcatctacgt gctgatctcc catgagggcg
     3841 ggtgggagac cctgtacggt catctctccg cgcggcgggt cgagttgaac acgaccgtga
     3901 aaagcggtat gatgatcggg aacgtcggtt ccaccgggct ctcgacgggc ccgcatctgc
     3961 atttcgaggt tcggatccgg ggagcgtccc gggatcccgt gccgcttcta ccacggttgg
     4021 gccaatgaaa cgttaccgcg ccctgacgat cccggccgcc ctggcggtcc tcctgttcct
     4081 cccgatcgcc tccgcgattc cggaaggcgt ccgaacggtc gtggccgcgg tggtagccgt
     4141 atccgcggac aagcccgacg gggaaaccgt gagcctcggc tatgcggacg ccgtggccgt
     4201 gactttgggc gcgggatcgc ccttcctcca gggcgtcgag atcgatctcc ggataccccg
     4261 acccctccag tccgcccagg gagccttcgc gtggtccctg tacaaggccg tcacccccgt
     4321 tccggcggtc gacaaagtgg cctacgacgg ggaccgcctc ctgatccagg ccctgcctcc
     4381 ccgggcggga gtcgtgttcc aggtccccgt ttcgccccgc cattccctgc gttccgggcc
     4441 ctacgccacc gtgctcccga tgctcctggg acccggggac ttcccgatcc tcttcaagct
     4501 gtccgccctg accaagggcg tgaccccgga gcaggaacag gcccggtacc aggtcaggat
     4561 ccgtcccctc ttcacggacg agggagccct gcggctctcc attctccccc cggagggagc
     4621 cgaaccggga ttcaccccct cggtctacgt ggacgagagg aagctggatt cctggcagga
     4681 tctggtcttc ctgaagaagg ggatgcacac cgtccaggtg gcgggggagg gcgtgcggga
     4741 cgaaagccgg gccgtggccg tggaagccgg caaggtgttc agcctggaga tccggctcca
     4801 gggcacccgt cccgtcgtgg tcttcgaggc ccccgcgaac gccctgatct tcctggacga
     4861 ccagcccgtg gaccacgcgg ggggcgcccg gatccaggtc gagccggggg agcgcaccgt
     4921 tgtctgccgg atcggggatt acac
//
LOCUS       DAOTEI010000222         4944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_12339_length_4944_cov_5.150133, whole genome shotgun sequence.
ACCESSION   DAOTEI010000222 DAOTEI010000000
VERSION     DAOTEI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12339_length_4944_cov_5.150133"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="P5313_15450"
     CDS             <1..259
                     /locus_tag="P5313_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRY81810.1"
                     /translation="FVRVLPEGADFTLMGIGRASLPAQYAAIARGGWIRVGFEDNVYY
                     SKGVLAESNAQFVARAARLAKEAGLTPATPADVRSHLKLRT"
     gene            436..1710
                     /gene="ablA"
                     /locus_tag="P5313_15455"
     CDS             436..1710
                     /gene="ablA"
                     /locus_tag="P5313_15455"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRY81811.1"
                     /translation="MNEYRTSDYKRIPLYANVPESDWNDWHWQMKNDIKDVETLSKVV
                     PLTEAEKKDIEAVLKRFRMAITPYYASLIDPADKHCPVRLQAIPRLPETFEDPADEDD
                     PLHEDVDSPVPGLTHRYPDRVLLLVTNICSMNCRHCTRRRLVGFEDVHMSDANIDAAV
                     EYIRKTPIVRDVLISGGDPLVLSDEKLERVIRKLREIEHVEVIRIGSRVPVVMPQRIT
                     DNLVSMLAKYHPIYLNTHFNHPKEITAEAKAACEKLANAGIPLGNQSVLLRDVNDCPM
                     TMKKLVQKLLTIRVKPYYIYQCDLSMGISHFRTSVSKGIEIIENLRGHTTGMAVPTFV
                     VDAPGGGGKTPVMPNYLITQNEKRIVMRNYEGVITTYTEPDTYVPRCPEGCEFCEDEL
                     LKPQDGVATLLAGQRLSLEPKDLKRFQRGHRP"
     gene            1718..2602
                     /locus_tag="P5313_15460"
     CDS             1718..2602
                     /locus_tag="P5313_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81812.1"
                     /translation="MLDRAEDFLGRVTFVTGPEKHCGKTTFLNRALSLVRAAGERPAF
                     LSIGYDGEARDSLSAARKPSVPVSPGDVVISAERFLRDGRILPEILEILPGSSAFGRI
                     CAARARRPGRIVLVGPEGNEGVARALAFLRDEGVARTVLVDGAINRITQAASWPGARF
                     VFVLRADAASLDKSARQARRLSSLASLPAAPPGFGSGEGEHILDGPLTAATAGALPDT
                     ARAVAVEDFTKVFLDDGELRSFRTGRELLVKAPVECAGIVAVLRGVSRPAFLSRLDGE
                     TAARIAFNPYELSPEAAA"
     gene            2599..4230
                     /locus_tag="P5313_15465"
     CDS             2599..4230
                     /locus_tag="P5313_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81813.1"
                     /translation="MSGRPAGLLAPEDLADAWSFSRAEEFWDLYRPYTPYGKDERDER
                     RVHSDRAAIEELLDRTEAAAAWLDSTREDPAARDRAGYYLKRMPRLPSSVKAEYELAE
                     IFQVKKFLADYQGLRTALDETARDAFGLGLAAPDLAAELGKGGSDPETFFVSDAYDPA
                     LAEVRMSIARTDADLEAARSAAETRLAARHGVRLSGRDFALVPRAQAEALLADRDLAV
                     VEPYDDRLYLVRPAADARILGLRDLRDELAARERKLEDDVIRRLSGLIASALPEIRAA
                     SERTRDFDLALARARLARDLGLVRPDLSAEGDWELTAGRFLPCEVECGDLGLAYTPLS
                     ARFGKPVTTIFGSNMGGKTVALKTVLFFQVLAQAGFHVPAASFRTRVYARLSYVGELA
                     GERLAGLSGFGFEIHRFIRAWEGMGEGGCLAVFDEFARTTSSREAEALLSAAQAELAA
                     RPGVKSFFATHFRGLVRSPGVDYLRMRGLDRKAASECLEADAPRAERLARINRHMRYE
                     LEPDAEGPGPADALTVAALLGLPGEIVKKAEGMLR"
     gene            4323..>4944
                     /locus_tag="P5313_15470"
     CDS             4323..>4944
                     /locus_tag="P5313_15470"
                     /inference="COORDINATES: protein motif:HMM:NF020610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HRY81814.1"
                     /translation="MRAKLGLSQDKISEARQLATRICEPVSKFVDGHTSVTVERATLR
                     LAGADDADADGVPVPNLIVDQARDRLADGAFRLYASALARTGDSPERLNRKIAEGLRL
                     TDLPPADESATARKAVELVNRAETRVRANREYRESKQAEWKDRNHRPLLYLIVATGNI
                     YEDVKQATAAAEQGADVIAVIQTTAQSLLDYVPFGPTTEGFGGTYAT"
BASE COUNT          773 a         1717 c         1715 g          739 t
ORIGIN      
        1 cttcgtccgg gtcctgccgg agggggcgga cttcactctg atgggcatcg gccgggcgtc
       61 cctgccggcc cagtacgcgg ccatcgcccg gggcggctgg atccgggtgg gcttcgagga
      121 caacgtgtac tactccaagg gcgtcctggc ggagtccaac gcccagttcg tggcccgggc
      181 cgcccggctg gccaaggaag cgggcttgac ccccgcgact ccggcggacg tgcggagtca
      241 tctgaaactc aggacttgat ttggaatgaa ccacggagtc gcgcgagccg gacgtaggtc
      301 cggctcgcgg cagcgaaacc cgttgggttt cgccgatttt agatgatttt ggcatcgccg
      361 aagcttgctt cggcgccgtc aggatggcgt agccatcctg acgcgtactc tgtggttgaa
      421 tcgtgaggag tctgtatgaa cgagtaccgt accagcgact acaagcgcat ccctctctac
      481 gcgaacgtgc cggagtcgga ctggaacgac tggcactggc agatgaagaa cgacatcaag
      541 gacgtggaaa ccctgtccaa ggtggtgccc ctcacggagg cggagaagaa ggacatcgag
      601 gcggtcttga agcgcttccg catggccatc accccctact acgcctccct gatcgacccc
      661 gcggacaagc actgtcccgt gcgcctccag gccatccccc gcctgccgga gaccttcgag
      721 gaccccgcgg acgaggacga tcccctccac gaggacgtgg actccccggt ccccgggctg
      781 acccaccgct acccggaccg ggtcctgctg ctggtcacga acatctgctc catgaactgc
      841 cgccactgca cccggcgccg actggtgggc ttcgaagacg tgcacatgtc cgacgccaac
      901 atcgacgcgg cggtggagta catccggaag accccgatcg tccgggacgt cctcatctcc
      961 gggggggatc ccctggtgct ctcggacgag aaactggagc gggtcatccg gaagctccgg
     1021 gagatcgagc acgtggaggt gatccgcatc gggtcccggg tccccgtggt gatgccccag
     1081 cggatcacgg acaacctggt ctccatgctg gccaagtacc acccgatcta cctcaatacc
     1141 cacttcaacc atcccaagga gatcaccgcg gaggccaagg cggcctgcga gaagctggcc
     1201 aacgcgggca ttcccctggg caaccagagc gtccttctgc gggacgtgaa cgactgcccc
     1261 atgaccatga agaagctggt ccagaagctc ctgaccatcc gggtcaagcc ctactatatc
     1321 taccagtgcg acctgtccat ggggatcagc cacttccgga cctcggtgtc caaggggatc
     1381 gagatcatcg agaacctccg cggccacacc acgggcatgg cggtccccac cttcgtggtg
     1441 gacgccccgg ggggcggcgg caagaccccg gtcatgccga actatctgat cacccagaac
     1501 gagaagcgca tcgtgatgcg caactacgag ggcgtgatca cgacctacac ggagccggat
     1561 acctacgtgc cccgctgtcc cgagggctgc gagttctgcg aggacgagct cctgaagccc
     1621 caggacgggg tggccacact gctggcgggg cagaggctct ccctggagcc caaggacctc
     1681 aagcgcttcc agcgcggcca ccgcccctag gcccgccgtg ctggaccggg cggaggactt
     1741 cctgggccgg gtcaccttcg tgaccggccc ggagaagcac tgcggaaaga ccaccttcct
     1801 caaccgggcc ctgtccctgg tgcgggccgc gggggagagg cccgccttcc tgtccatcgg
     1861 ctacgacggg gaggcccggg actccctctc ggcggcccgc aagccctcgg tgcccgtgtc
     1921 cccgggggac gtggtgatca gcgccgagcg cttcctgcgg gacgggcgca tcctcccgga
     1981 gatcctggaa atccttcccg gaagctccgc cttcggccgg atctgcgcgg cccgggcgag
     2041 gcggccgggc cgcatcgtcc tggtgggccc cgagggcaac gagggcgtgg cccgggccct
     2101 ggcctttctc cgggacgaag gcgtcgcccg gacggtcctg gtggacgggg cgatcaaccg
     2161 gatcacccag gcagcctcct ggccgggggc gcgcttcgtc ttcgtgctcc gggccgacgc
     2221 cgcctcactg gacaagtcgg cccggcaggc ccgacgcctc tcgtccctgg cttccctgcc
     2281 cgcggcgccc ccgggcttcg gttcggggga gggggagcac atcctggacg gccccctcac
     2341 cgcggcgacc gcgggggccc tgccggatac cgcccgggct gtggcggtgg aggacttcac
     2401 caaggtcttc ctggacgacg gggagctccg gtccttccgg accgggaggg agcttctcgt
     2461 caaggcgccc gtcgagtgcg cggggatcgt ggcggtcctg cggggggtat cccgcccggc
     2521 cttcctgtcc cggctggacg gggagaccgc ggcccggatc gccttcaacc cctacgagct
     2581 ctccccggag gccgcggcat gagcggccgg cccgccggcc tcctggcgcc cgaggacctc
     2641 gcggacgcct ggtccttctc ccgggccgag gagttctggg acctctaccg tccctacacc
     2701 ccctacggca aggacgagcg ggacgagcgg cgggtccatt cggaccgggc cgcgatcgag
     2761 gaactcctgg accggaccga ggccgcggcg gcctggctgg attcgacccg ggaggacccc
     2821 gcggcccggg accgggcggg atactacctg aagcggatgc cccggctccc gtcttcggtc
     2881 aaggccgagt acgagctggc ggagatcttc caggtgaaga aatttctggc cgattaccag
     2941 ggtctccgta cggccctgga cgaaaccgcc cgggacgcct tcggcctggg gctcgcggcc
     3001 ccggacctgg ccgcggagct gggcaaggga ggctcggacc cggagacctt cttcgtctcc
     3061 gacgcctacg atcccgccct ggcggaggtc cggatgtcca tagcccggac ggacgcggac
     3121 ctggaggcgg cccggtcggc cgcggagacc cggctggccg cccggcacgg ggtacgcctt
     3181 tccggccggg acttcgccct ggtcccccgg gcccaggcgg aggccctgct ggcggaccgg
     3241 gacctggcgg tggtggagcc ctacgacgac cgcctctacc tggtgcggcc ggcggcggac
     3301 gcccggatcc tggggctccg ggacctccgg gacgagctgg ccgcccggga acgcaaactg
     3361 gaggacgatg tcatccggcg cctctcgggc ctcatcgcaa gtgcgctccc ggagatccgg
     3421 gccgcctcgg agcggacccg ggacttcgac ctggccctgg cccgggcccg ccttgcgcgg
     3481 gacctgggac tcgtccggcc ggacctctcc gccgagggtg actgggaact cacggccggc
     3541 cgcttcctgc cctgcgaggt ggagtgcggg gatctcggcc tggcgtatac gcccctgtcc
     3601 gcccggttcg gaaagccggt gaccacgatc ttcgggtcca acatgggggg caagaccgtg
     3661 gccctgaaga cggtgctctt cttccaggtc ctggcccagg cgggtttcca cgtccccgcc
     3721 gcctccttcc gcacccgggt ctacgcccgg ctctcctacg tgggggagct ggcgggggag
     3781 cgcctggcgg ggctctcggg cttcggcttc gagatccacc gcttcatccg ggcctgggag
     3841 gggatggggg aaggcggctg cctggcggtc ttcgacgagt tcgcccggac caccagttcc
     3901 cgggaggcgg aggccctcct gtccgcggcc caggcggagc tggcggcccg gcccggggtg
     3961 aagagcttct tcgccaccca cttccggggc ctggtccggt cgccgggggt ggactacctg
     4021 cggatgaggg gcctggaccg gaaggcggcc tcggaatgcc tggaggcgga cgccccccgg
     4081 gcggagcgcc tggcccggat caaccggcac atgcgctacg aactggaacc ggacgcggag
     4141 ggccccggcc ccgcggacgc cctgaccgtc gcggccctgc tgggcctgcc gggggagatc
     4201 gttaagaaag cggaaggaat gttgaggtaa acgtgaatgg gtgaataggt gaatgagtga
     4261 atggggtttc cttctattca cccattcact attcacttat tcaccttcca caaggagttt
     4321 tcatgcgcgc gaaactaggc ttatcccaag acaagatctc cgaagcccgc cagctcgcga
     4381 ccaggatctg cgagccggtg tcgaaattcg tggacggcca cacctccgta acggtggagc
     4441 gggccactct gcgcctggcg ggggcggacg acgcggacgc cgacggggtg cccgtgccga
     4501 acctgatcgt ggatcaggcc cgggaccgtt tggcggacgg tgccttccgc ctctacgcga
     4561 gcgccctggc ccgcacgggg gacagcccgg agcggctgaa ccggaagatc gcggagggct
     4621 tgcgcctgac cgacctgccc cccgcggacg agtccgcgac ggcccggaag gcggtcgagt
     4681 tggtgaaccg ggccgagacc cgggtccgtg ccaatcgcga gtaccgggaa tccaagcagg
     4741 cggagtggaa ggaccggaac caccggcccc tcctgtacct catcgtggcc acgggcaaca
     4801 tctacgagga cgtgaagcag gccacggccg cggcggagca gggggcggac gtgatcgccg
     4861 tgatccaaac caccgcgcag agcctcctcg attatgtacc cttcggcccg accaccgagg
     4921 gcttcggggg gacctacgcc accc
//
LOCUS       DAOTEI010000223         4904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_12475_length_4904_cov_4.503403, whole genome shotgun sequence.
ACCESSION   DAOTEI010000223 DAOTEI010000000
VERSION     DAOTEI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12475_length_4904_cov_4.503403"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="P5313_15475"
     CDS             complement(<1..471)
                     /locus_tag="P5313_15475"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY81815.1"
                     /translation="MASKEPAAQDKAAREEKPSIHARLNDFLRKNRAAVITVFAAAVL
                     AVVAAAVWTVVDERVDTRSAQALEKVELSLGEWYGMEQGPAAAEKANEILAALDTIRS
                     KYGKRYAGQKALVLAGRVQARNGDWAAAEKLFLQAADLNNKTALAGFALQEAATA"
     gene            complement(475..1986)
                     /locus_tag="P5313_15480"
     CDS             complement(475..1986)
                     /locus_tag="P5313_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY81816.1"
                     /translation="MLERIDPRKFETLIEINTLINSNYADATSLLTHIIESATRLTEG
                     EASSLVLKNPEDGKLYFEIALGPKGRDVKKFSLNPGEGIAGWVAAHTQSLIVNDVETD
                     SRFYGEISKSIGFPTFSILAVPMVVRDNCVGVIEILNKKGKKYFTQEDLQWLEIFAVQ
                     AAIAIENARYLERAREEIGYLREQIDIDKGYHVLVSASPLIKEKLDLIERVAPTDSSV
                     LILGESGVGKELFAEQLHLRSGRHKGPFVRVNCAALPEGLLESELFGHVKGAFTDAVQ
                     NRRGRFELADGGTIFLDEIGDLPLKLQAKLLRVLQQKTFERVGSSDSVTVDVRIVAAT
                     NRDIESLVEKGEFRSDLYYRLNVLPIYVPPLRQRKEDIPALAEYFLKKFGRETKKQFR
                     GFTDQAMDIMLSYSWPGNVRELENAVERACVIAKDQYIGVPDLLLGGRAESADPYGGK
                     SLKDSLAIFKRHFIRRALEDHDWNQTETAKVLDIQRTYLSRLIKELSINDYKE"
     gene            complement(1993..4359)
                     /gene="rpsA"
                     /locus_tag="P5313_15485"
     CDS             complement(1993..4359)
                     /gene="rpsA"
                     /locus_tag="P5313_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRY81817.1"
                     /translation="MIVAIDGPAGTGKSTIARMVAERLGITYVNSGSLYRALTYGLLS
                     AGLDPSEEGPVVEWARKARLEYRDGRVHLDGRDVEAFLRTDAVEAWVAQVSAFVPLRH
                     IVNGVVRDLSRSMDIVVEGRDMTTAVFPDAEHKFFLDATAESRALRRLSQGTSMSSLE
                     EIRQNIEMRDRIDREKPEGPLKVSSDALYLDTSHLTIEEVYEKVYTKIQQQGNIMGVK
                     EVGMEGSATPEDNFQTQLQEQYLKSMDHLEEGELVDGQVIEVSSDCVFIDVGAKSEGK
                     IPLVEFDTPPKMGDTVSVVLLKKETYSGEIAVSKKRADEKIRWRIISNAFRDNQPVDG
                     VIEKEIKGGFDVNLGPNLRAFLPVSKADTQRVEKGDYLVGVKTKFYVERLYSEKRVNL
                     VVNRRKWLEEQTEKNREEFFSKVQIGDTVKGEVKSFTSFGAFIDLGGFDGLLHINDMS
                     WGHVTRPKDFVKKGQEIELKVIRMDPEEKRINLSLKHFTEDPWISFEERYHVGDVVNG
                     KVTKLTDYGAFIEVEEGIEGLAHISEFSWVRKVRKPEEMLRIGDPVQCMILAYDLQAG
                     KVSLGLKQVQENPWDQVSEKYPVGMRLARKVVKLTNAGAFIELEEGIDGFLHVDDISW
                     TKRVKHPGSELQVGQEIEVMVIDSDQDNRNIRLGIKQLSEDPWHSFATAFHVGSIVDG
                     TISSITDFGLFVKVQGDIEGLVNKSNLSTDRDEAFEDAIKKFEVGQAIKTVIVELSPD
                     RQKLSLSVRDLVKKQQREEISRFMQDDAAEGYTLGDMLKERGGSGKNS"
     gene            complement(4356..>4904)
                     /locus_tag="P5313_15490"
     CDS             complement(4356..>4904)
                     /locus_tag="P5313_15490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRY81818.1"
                     /translation="LNKPAGVLSAASDPHGRPVAVDLIKPEVTERVYNVGRLDYDSEG
                     LLLFSNDGEFAGAVGHPSANTEKEYRVRTDRPVPEGFAKAFLRGIPEGGQILRARSVE
                     AEDERTLRVTLIEGRNREIRRALAAFGLAAVRLVRVRVGPVTLEGLRPGAWRDLTEPE
                     KEALRNAAPDPASNTFYRSRPS"
BASE COUNT          767 a         1590 c         1528 g         1019 t
ORIGIN      
        1 ggccgtggcc gcttcctgca gggcgaatcc cgccagggcg gtcttgttgt tcagatccgc
       61 ggcctggagg aagagcttct cggcggcggc ccagtcgccg ttccgggcct ggaccctgcc
      121 ggccaggacc agggccttct gtcccgcgta acgcttcccg tatttggatc ggatggtgtc
      181 gagggcggcc aggatctcgt tggccttttc cgcggcggcc ggtccctgct ccatcccgta
      241 ccattcccca agggaaagct cgaccttttc cagagcttgg gcggaccggg tgtcgacgcg
      301 ctcgtcaacg acggtccata cggccgcagc gacgacggcc agaacggccg cggcgaatac
      361 ggtgatcacg gccgcacggt tcttccggag gaagtcgttc agtcgggcat ggatgctggg
      421 tttttcctcc cgcgcggcct tgtcctgcgc ggcgggttcc tttgaggcca tatatcactc
      481 cttgtagtcg tttatcgaga gttccttgat caatcgggag aggtaggtcc tctggatatc
      541 caggaccttt gccgtctccg tctggttcca gtcgtgatcc tccagggccc ggcggatgaa
      601 atgccgcttg aagatcgcca gggaatcctt gaggctcttt ccgccgtacg gatcggcact
      661 ctcggcgcgc cctcccagca ggaggtccgg aaccccgatg tactggtcct tggcgatcac
      721 gcaggcccgc tccaccgcgt tctccagctc ccggacgttc ccgggccagg agtaggacag
      781 catgatgtcc atggcctggt cggtgaagcc ccggaactgt ttcttggtct cccgtccgaa
      841 cttcttcagg aagtactccg ccagggcggg aatgtcctcc ttgcgttgcc ggaggggcgg
      901 aacgtagatg ggaaggacgt tcagccggta atacaggtcg ctgcggaact cccccttctc
      961 caccagggac tcgatatccc ggttcgtggc cgccacgatg cgcacatcca cggtcacgga
     1021 gtcgctggag cccacgcgct cgaaggtctt ctgctggagc acccggagga gcttggcctg
     1081 gagcttgagc ggcagatccc cgatctcgtc caggaagatc gtgccgccgt cggccagctc
     1141 gaaacggccc cgccggttct gcacggcgtc cgtgaaggcg cccttgacgt ggccgaagag
     1201 ctcgctttcc aggagtcctt ccggcagggc cgcgcagttc acccgcacga agggtccctt
     1261 gtgccggccg gaccggaggt ggagctgttc tgcgaaaagc tccttaccca ccccgctctc
     1321 ccccaggatc aggaccgagg agtccgtcgg cgcgacccgc tcgatcagat ccagcttttc
     1381 cttgatcagg ggactggcgg acacgaggac gtggtagccc ttgtcgatgt cgatctgctc
     1441 ccggaggtag cctatctcct cccgggcccg ctcgaggtac cgggcgttct cgatcgctat
     1501 ggccgcctgc accgcgaaga tctccagcca ctggagatct tcctgtgtga agtacttctt
     1561 gcccttcttg ttgaggatct ctatgacccc cacgcagttg tcccggacca ccatgggcac
     1621 cgccaggatg ctgaaggtgg ggaaaccgat gctcttggag atttccccgt agaatcggct
     1681 gtcggtctcc acgtcgttca cgatcaggga ttgggtgtgg gcggccaccc agccggcgat
     1741 gccttccccg ggattcaggg agaatttctt gacgtcccgg cctttgggcc ccagggcgat
     1801 ctcgaagtaa agcttcccgt cctccgggtt cttgagcacc aggctggagg cctcgccctc
     1861 ggtcagccgc gtggcggact cgatgatgtg ggtcagcagg gacgtagcgt ccgcgtagtt
     1921 cgagttgatc agggtgttga tctcgatgag ggtctcgaac ttacgcgggt cgatacgttc
     1981 cagcatgtcc ggttacgagt tcttgcccga gccgcccctc tccttgagca tgtcgccgag
     2041 ggtgtacccc tccgccgcgt cgtcctgcat gaagcgggag atctcttccc tctgctgctt
     2101 cttcaccagg tcgcgcacgg acagcgagag cttctgccga tccgggctca gttccacgat
     2161 gaccgtcttg atggcctgtc cgacctcgaa cttcttgatc gcgtcctcga aggcctcatc
     2221 ccggtccgtc gagaggttgg acttgttcac caggccctcg atgtcgccct ggaccttcac
     2281 gaacagtccg aagtccgtga tcgaggagat cgtgccgtcg acgatgctgc ccacgtggaa
     2341 ggcggtggcg aaggagtgcc agggatcctc ggacagctgc ttgatgccca gccggatgtt
     2401 ccggttgtcc tggtccgaat cgatgaccat aacctcgatc tcctgcccga cctggagctc
     2461 ggaaccgggg tgcttgaccc gcttggtcca ggagatgtcg tccacgtgga ggaagccgtc
     2521 gatgccttcc tcgagctcga tgaaggcccc ggcgttggtg agcttcacga ccttccgggc
     2581 caggcgcatt cccacggggt acttttcgga aacctggtcc caggggtttt cctggacctg
     2641 cttgagcccc agggagacct tgcccgcctg gaggtcgtag gccaggatca tgcactgcac
     2701 ggggtcgccg atccggagca tttcctcggg cttgcgtacc ttgcggaccc aggagaactc
     2761 ggagatgtgg gccaggccct cgatgccctc ctcgacctcg atgaaggcgc cgtagtccgt
     2821 gagcttggtg acctttccgt tcaccacgtc gcccacgtgg taccgctcct cgaaggagat
     2881 ccaggggtcc tcggtgaagt gcttgaggga gaggttgatc cgcttctcct cgggatccat
     2941 gcggatgacc ttgagctcga tctcctggcc cttcttcacg aaatccttgg gccgggtgac
     3001 gtgcccccag ctcatgtcgt tgatgtgcag gagcccgtcg aagccgccca ggtcgatgaa
     3061 ggcgccgaag gaggtgaagc tcttgacctc gcccttcacg gtgtccccga tctggacctt
     3121 ggagaagaac tcctcgcggt ttttctcggt ctgctcctcg agccacttgc gccggttcac
     3181 gaccaggttg acccgcttct cggaatagag gcgctccacg tagaacttgg tcttgactcc
     3241 gacgaggtag tcgcccttct ccaccctctg ggtgtccgcc ttggatacgg gcaggaaggc
     3301 ccggaggttc gggccgaggt tgacgtcgaa gccgcccttg atctccttct cgatgacgcc
     3361 gtccacgggc tggttgtccc ggaaggcgtt ggagatgatg cgccagcgga tcttttcgtc
     3421 cgcgcgcttc ttggagacgg cgatttcccc ggaataggtc tccttcttga ggaggaccac
     3481 gctgaccgta tcccccatct tggggggagt atcgaactcg acaaggggga tcttcccctc
     3541 ggacttggcc cccacgtcga tgaagacgca gtcgctggag acttcgatca cctgaccgtc
     3601 gacgagttcc ccctcttcga ggtgatccat ggacttgaga tactgttcct gcaattgtgt
     3661 ctggaaattg tcctcggggg tcgcggaccc ttccattccc acttccttca cacccataat
     3721 gttcccttgc tgttgaattt tcgtgtacac tttctcataa acttcctcta tggtcaagtg
     3781 agaggtatcc aaatacaggg cgtccgaaga gactttcagg ggtccctccg gcttttcgcg
     3841 gtcgatccgg tccctcatct cgatattttg tctaatctct tccaggctcg acatgctggt
     3901 cccctgggaa agccgtcgca gagcccggct ctccgcggtg gcgtccagga agaacttgtg
     3961 ttccgcgtcc gggaagaccg cggttgtcat gtcccgcccc tccacgacga tgtccatgct
     4021 ccgggacagg tccctgacca caccgttgac gatatgccgg agggggacga aggcgctgac
     4081 ctgtgccacc caggcctcca cggcgtcggt ccgcaggaag gcctccacgt ccctcccgtc
     4141 caggtggacc cggccgtccc gatactccag ccgggctttc cgcgcccatt ccacgacggg
     4201 gccttcctcg gagggatcca ggccggcgga gagcaggccg taggtcaggg ccctgtacag
     4261 gcttccggag ttgacgtacg tgatgcccag gcgctcggcg accatgcggg ctatggtgct
     4321 cttgccggtt ccggcggggc cgtcgatggc tacgatcatg acgggcggct cctgtagaag
     4381 gtattcgagg ccggatccgg ggcggcgttc cggagggcct ccttctccgg ttccgtaagg
     4441 tctctccagg cgcccggacg gagcccctcc agggtcacgg gacccacccg cacccggacg
     4501 agccggaccg cggccaggcc gaaggcggcc agggcccggc ggatctcccg gttacgtccc
     4561 tcgatcaggg tcacccgcag tgtccgctcg tcctcggcct cgacggaacg ggcccggagg
     4621 atctgtccgc cctccgggat cccccgcagg aaggccttgg cgaagccctc cggtacgggc
     4681 cgatccgtac gcacccggta ctccttctcg gtgttggcgg aaggatggcc cacggccccc
     4741 gcgaattcac cgtcgttgga gaacaagagt agcccctcgg agtcgtagtc caggcgcccc
     4801 acgttgtaga cccgctcggt cacctcgggc ttgatcaggt cgacggcgac gggccggccg
     4861 tgggggtccg aagccgcgga gagcaccccc gcgggcttgt tcag
//
LOCUS       DAOTEI010000224         4857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_12650_length_4857_cov_5.235943, whole genome shotgun sequence.
ACCESSION   DAOTEI010000224 DAOTEI010000000
VERSION     DAOTEI010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12650_length_4857_cov_5.235943"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2240
                     /locus_tag="P5313_15495"
     CDS             <1..2240
                     /locus_tag="P5313_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRY81819.1"
                     /translation="KAVSLLFAVDSPDFGRSGDNPELEALLPVDLLPRSLKRLPDLAV
                     LLLLDVSGSMFGDKLSLAKAAGVEFLKALKPTDRVGLLLFSEERSWLYRFEPNSSIQP
                     GRDIPNLAAGGGTDLAAALREGLGELSKFRDRDRHAVVISDGVTKPADFRPIEEAAAR
                     AGISLSTIAVGKDADVSLLGRLAKNTGGRAYRADRFDQIPALLFEDRKSVSRTSFSRE
                     TQSVLALNGDKVSTVEGMALLTPRDPSTILLANELGDPLLASREYRNRGVVVFASDLY
                     GAYTADFFRSPGAVSYLKRRLDALLAEESVPVSVHEHAGGVELVLRSPWLVSPEVRVL
                     YADRRVAREDPLKRSAPGVYGAALPLSERGLYTAVLSDRGAAFARVPVAMNGVFRAVP
                     ASAYRAVREYRPARLVRYPGRGAWLVAFFLSSLGVTVLLRLDGSGARTAAVLIAALTV
                     SARLGAEPEIRFDEVGYRPYGICSGTVRSPEGFSGTLELRGRRFDGRDVTLFRAEIPG
                     WTASLEFRFIPDEDVRAVYVAGLGGERGLPLPPGGTEETAGRRFLASLSPPFRSRPPA
                     RLPGSARLRARDPGEAGSEAARLFGVPPEPAAAGILAAFAAGALALAAFRGRMGRAYL
                     PALLVLAAAGAAGMAAARPSAVLFRFPLPGPRGPYDLEVRTGIERSPGLSVVRFDPSG
                     NGPTRPSLTVLGIRTPPGRSVPLSAFPEGALFRFRDPPLVLGDGDGSFSLRFEGFALG
                     WELLE"
     gene            2233..2712
                     /locus_tag="P5313_15500"
     CDS             2233..2712
                     /locus_tag="P5313_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81820.1"
                     /translation="MNRRILRAAAVLAPLGTAALLMPLADRPLPAWAASSLLAMAVRI
                     LDGAAGRAFGRPDPSFLPEAPLWAAPFAVRILSLLSAPGFPDLFTASALWAGFYAAAA
                     AAECAASFRGRRGFPAFAAALAGLAAFTAVAGSPAGTAGAALTAAGLVYSARRRLAP"
     gene            2709..3878
                     /locus_tag="P5313_15505"
     CDS             2709..3878
                     /locus_tag="P5313_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81821.1"
                     /translation="MIGPRAYSMLRRRAGAWTRRSLAGSLALWGLLVLAASNLLSAAG
                     LLGWIGRAGHDGLLLLVISTFPLALFRPPDPRSGLVELRTVDRGAVLESALAAAPGDA
                     AEPLLRRTGEGAAEAAGRIRPKRRPTPRGTRFLLLAAGGIILVSQGTFLFVAGRPVIG
                     YASLQESRGRAEEGAEAFARPESPDPAEGDGLPVPEPARDRDPEARLENPAERARREA
                     EFADLHGLLRQDREGLAPEPREGEAAEEGNAPPPAAPAGGDGPPGGGSGGTGRAGAPG
                     PDAPAPEGSGGAGDPRGTGGQERNQGPGSGWSDSPGAQARNAMKDYRSAFEKILTERT
                     GAAVTAGQDLSLAETEAAVRLYFDSLRLRVEVDPQEDPLAAGLRAAWQRLRGGIR"
     gene            3875..4537
                     /locus_tag="P5313_15510"
     CDS             3875..4537
                     /locus_tag="P5313_15510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRY81822.1"
                     /translation="MRMRRTGRGFPGLREGIPAALRDAGTRAAAILRRPGGPVLTAVF
                     AAAGAGLYPAAAAINRFLADRFALPFAVSVLPEELLKLGMAGAAAALARRLGDPGKGL
                     AAVAGATGFAALENLAYLGAFPGAGVFLRLGWSLPLHVNGAALFALALASRRPGAAAA
                     AALLASAAYHAAFNAAAAAAPAPLVTAGGVAINLVVCAGLAFAARLRFAWGGILDGKP
                     RL"
     gene            4518..>4857
                     /locus_tag="P5313_15515"
     CDS             4518..>4857
                     /locus_tag="P5313_15515"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY81823.1"
                     /translation="MESRDFEAALERVRSFYEAYAAYVGARVVGYPELLERLFTAFLS
                     GGHVLLEGAPGLGKTSLAKSWAAFFGLEFRRVQFTPDLMPLDVIGSNILQDGAEGREF
                     RFYRGPIFANV"
BASE COUNT          549 a         1790 c         1770 g          748 t
ORIGIN      
        1 gcaaggcggt cagcctgctc ttcgccgtcg attccccgga cttcggccga tccggggaca
       61 accccgagct ggaggccctg ctcccggtgg acctgcttcc ccggtccctg aagcggcttc
      121 ccgacctggc cgtcctcctc ctgctggacg tatcgggcag catgttcggg gacaaactgt
      181 ccctcgccaa ggccgcgggt gtcgaattcc tcaaggccct caagcccacg gaccgggtcg
      241 ggctcctcct cttctcggag gaacgctcct ggctgtaccg tttcgagccg aactcctcca
      301 tccagcccgg gagggatatc cccaacctgg ccgcgggagg ggggacggat ctggcggcgg
      361 ccctccggga gggactcggg gaactgtcga aattccggga ccgggaccgg cacgcggtgg
      421 tcatctccga cggggtcacg aagccggcgg atttccggcc catcgaggag gcggcggcgc
      481 gggccggaat aagcctttcc accattgccg tgggcaagga cgccgacgtc tccctcctgg
      541 ggcggctggc caagaacaca gggggccggg cctaccgggc ggaccgcttc gaccagatac
      601 ccgccctgct cttcgaggac cggaaaagcg tgtcgaggac ctccttctcc cgggaaaccc
      661 agtccgtcct ggccctcaac ggggacaagg tctccacggt ggaggggatg gcactgctga
      721 cgccgaggga cccctccacg atcctcctgg ccaacgaact gggggatcct ctcctggctt
      781 cccgggaata ccggaaccgg ggggtcgtgg tcttcgccag cgacctgtac ggagcctata
      841 cggcggactt cttccgatcc cccggggccg tatcctacct gaaacggcgt ctggacgccc
      901 tgctggccga ggagagcgtg ccggtttctg tgcacgagca cgcggggggg gtggagctcg
      961 tcctgcgaag cccctggctc gtgtccccgg aggtccgggt cctgtacgcg gatcggaggg
     1021 tcgcccggga ggatcccctc aagcgtagcg ccccgggggt ctacggcgcg gccctccccc
     1081 tgtccgaacg gggcttgtac acggcggtcc tttcggaccg cggagccgcc ttcgcccggg
     1141 tccccgtcgc gatgaacggt gtgttccggg cggtcccggc gtcggcgtac cgcgcggtcc
     1201 gggagtacag gccggcccgc ctggtccggt accccggccg cggcgcctgg ctggtcgcct
     1261 tcttcctgtc gtccctgggg gtgacggtcc tgctccggct ggacggatcc ggcgcccgga
     1321 cggcggccgt cctgatcgcg gccctgacgg tttccgcgcg cctgggggcg gaaccggaga
     1381 tccggttcga cgaggtgggc taccgtccct acgggatctg ctcgggaacc gtccgctccc
     1441 ccgagggctt ttccgggacc ctggaactca ggggccggcg attcgacggc cgggacgtga
     1501 ccctgttccg ggcggagatc ccggggtgga ccgcttccct ggagttccgg ttcattcccg
     1561 acgaggacgt ccgggcggtc tacgtcgccg ggctgggcgg ggagcgcggt cttccccttc
     1621 ctccgggcgg gacggaggag accgcgggac ggcgcttcct ggcctccctt tccccgccgt
     1681 tccgctcccg tccgcccgcc cgccttccgg gatcggcccg cctccgggcc cgggaccccg
     1741 gggaggccgg gtcggaggcc gcccggctct tcggcgtccc ccccgagccg gcggccgcgg
     1801 gcatcctggc ggccttcgcg gcgggggccc tcgccctcgc ggccttccgg ggccgaatgg
     1861 gccgggccta ccttcccgcg ctcctggtcc tggccgcggc gggagcggcg ggcatggccg
     1921 cggcccggcc gtccgccgtc ctcttccggt ttccccttcc cggcccgagg ggtccctacg
     1981 acctggaggt ccggaccggg atcgagcgga gtcccggcct gtccgtagtc cggttcgatc
     2041 cgtccggaaa cgggccgacc cgtccctccc tcacggtcct ggggatccgc actcctcccg
     2101 ggcggtccgt gccgctgtcg gccttcccgg aaggggccct gttccggttc cgggatcctc
     2161 ccctcgtcct gggggacggc gatggctcgt tctcgctgcg tttcgagggc ttcgccctgg
     2221 ggtgggaact ccttgaatag gcggatcctc cgggctgcgg ctgtcctggc tcccctgggg
     2281 acggcggccc tccttatgcc cctggcggac aggccccttc ccgcctgggc cgcctcgtcc
     2341 ctgctggcta tggccgtccg gatcctggac ggagcagcgg gccgcgcctt cggacggccc
     2401 gatccctcgt tcctgccgga ggcccccctc tgggccgcgc ccttcgcggt ccggatactc
     2461 tccctcctgt ccgctccggg attcccggat ctttttacgg cctcggccct ctgggccggg
     2521 ttctacgcgg ccgccgcggc ggcggaatgt gccgcttcct tccgcggccg ccgaggattc
     2581 ccggccttcg cggccgccct cgcgggcctg gccgccttca cggccgtcgc cgggtcgccc
     2641 gcggggaccg ccggggccgc gctgacggcg gccggcctgg tctattcggc ccgcaggagg
     2701 ctcgccccgt gatcggaccc cgggcgtact ccatgctccg gcggagggcc ggggcctgga
     2761 cccggagatc cctggccgga tccctggccc tctggggcct cctggtcctg gcggcctcca
     2821 acctcctctc cgccgcgggc ctcctgggct ggatcggccg ggcgggccac gacggacttc
     2881 tccttctcgt gatctcgacc tttcccctgg ccctcttccg gcccccggac ccgagatcgg
     2941 gcctagtaga actgcggacc gtggaccggg gcgccgtcct ggaatcggcc ctggccgctg
     3001 ccccggggga cgccgcggaa cccctgctcc ggaggaccgg ggaaggagcg gcggaagccg
     3061 caggacggat ccgacccaag cggcgcccga cgccccgggg tacgcgtttc ctgctcctgg
     3121 ccgcgggggg gatcatcctg gtctcccagg gaaccttcct cttcgtcgcg gggaggcccg
     3181 tgatcggata cgcgtccctc caggagagcc ggggccgggc ggaggaagga gccgaggcct
     3241 tcgcgaggcc ggaatccccc gaccccgccg agggggacgg ccttcccgtg ccggagcccg
     3301 cccgggaccg ggatccggag gcccggttgg aaaatccggc ggagcgggcc cggagggagg
     3361 ccgagttcgc ggacctccac ggcctgctga ggcaggacag ggagggactc gccccggagc
     3421 cccgggaagg ggaggcggcg gaggaaggga acgccccgcc tcccgcggcc ccggccggag
     3481 gggacgggcc cccgggcgga ggctccggcg gcacgggccg tgccggtgcg cccggtccgg
     3541 acgccccggc gcccgaggga tcgggaggag ccggggatcc ccggggaacc ggcggccagg
     3601 aacggaacca aggccccggc tcgggctggt ccgactcccc gggagcccag gcacggaacg
     3661 ccatgaagga ttacaggtcc gccttcgaga agatcctgac cgagaggacc ggggcggccg
     3721 taacggcggg gcaggatctt tccctggcgg aaacagaagc cgccgtacgc ctctacttcg
     3781 attccctgcg ccttcgggtg gaggtggacc cccaggagga tcccctggcc gcgggcctgc
     3841 gggcggcctg gcagcgtctc cggggaggaa tccgatgagg atgcggcgaa cgggccgggg
     3901 tttcccggga ctccgggaag ggatcccggc ggcgctccgg gacgcgggga cccgggcggc
     3961 cgcgatcctt cgccggccgg gcgggccggt cctgacggcc gtgttcgccg ccgcgggggc
     4021 cgggctgtat ccggcggcgg ctgcgatcaa ccggttcctg gcggaccgct tcgccctgcc
     4081 cttcgccgtc tccgtcctgc cggaggagct tctcaaactg ggaatggccg gggcggccgc
     4141 ggccctggcc cgccgcctcg gggatccggg gaaggggctg gcagccgtgg cgggggcgac
     4201 gggattcgcg gccctggaaa acctggcgta tctcggtgcc ttcccgggag ccggggtctt
     4261 cctgcgcctc ggctggagcc tgcccctcca cgtcaacggt gcggccctct tcgccctggc
     4321 cctggcgtcc cggcgtcccg gagccgcggc cgccgccgca ctgctcgcat ccgccgcata
     4381 tcacgccgcc ttcaacgccg ccgccgcggc ggccccggcc cccctcgtga cggcgggagg
     4441 ggtcgcgatc aacctcgttg tctgcgcggg cctcgcgttc gcggcccggc tacgattcgc
     4501 atggggaggg atcctggatg gaaagccgag actttgaggc cgccctggaa cgggtgcgct
     4561 cgttctacga ggcctacgcc gcctacgtgg gagcccgggt ggtcgggtat cccgaactgc
     4621 tggaacgcct cttcacggcc ttcctctccg gcggccacgt gctcctggag ggggctccgg
     4681 gcctgggcaa gacgagcctg gccaagtcct gggccgcctt cttcgggctg gagttccgcc
     4741 gggtccagtt caccccggac ctcatgcccc tggacgtgat cggatccaac atcctgcagg
     4801 acggggccga ggggcgggag ttccggttct accggggccc gatcttcgcg aacgtga
//
LOCUS       DAOTEI010000225         4782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_12941_length_4782_cov_4.502433, whole genome shotgun sequence.
ACCESSION   DAOTEI010000225 DAOTEI010000000
VERSION     DAOTEI010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12941_length_4782_cov_4.502433"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="P5313_15520"
     CDS             <1..470
                     /locus_tag="P5313_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81824.1"
                     /translation="KEAGTSPAPELAVKWPNDLLVRGRKAAGILCESSGGRCCLGMGV
                     NCGTPSGRGFRTEATGLAEELKAPVDRFRVLELILERLAEALAEEGWREEVSRRLWLL
                     DRPARFRTGLADPGAPGTRILEGIVRGVGPSGELILETAEGIRALASGELSRD"
     gene            542..2104
                     /locus_tag="P5313_15525"
     CDS             542..2104
                     /locus_tag="P5313_15525"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HRY81825.1"
                     /translation="MATKSPGPSAAGTPSETGILARILSIFTGGDPEAEKKKLLRSIG
                     KDLSRSRYKFYKPKGSEALPGLGRFFYEIYKIVAPAQILLTNAAGSGVLRSFVIESFL
                     SPEQKEISERLTDAWIQEKAKTLSVKELSELVKQDMIRFISVFDGDLTARIDTAHATL
                     VALANFVNFDYFFLLKKFDSSIPERNFTYHPKFETISADYIVDDLKDFLEVFLALNLE
                     ADWKRILGALKEYRNIDVLPVDPWIKLTGALNEVRQSRILEQVVRHAQKDPYWTAAPR
                     SQADRIVEPFLEKLRSQMEIQLQKIAQERRNSKIDDLAKQVFGTSVVVRMKNYTDKAN
                     VVFAKKMMGGFTQTAALNYIRAFLIDFFKKDVRELVDLLIIRGQWTTSILSQQLSDAY
                     HALLELSDEIIRFDDSLGDDGDLGNKLRNVLAKSDRDKEQLKYLRNLLKDTNDRASGM
                     VNRSALNLISVGKHLKSLIEDLSRPHHEVVLNWKEIENSAAHPVKEWMLGTYKKIYYL
                     VQLLQFFVSKEQ"
     gene            complement(2114..3388)
                     /gene="hflX"
                     /locus_tag="P5313_15530"
     CDS             complement(2114..3388)
                     /gene="hflX"
                     /locus_tag="P5313_15530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRY81826.1"
                     /translation="MTELTETAARPERALLIGIQTDSIPKPEAESLLAELASLAKTQG
                     IQEAGRILVRIRDRTASHLLSSGKVQELVRAAEAEDADSIIFDHPLSPVQQRNWEEDS
                     GRVVYDRSELILKIFAGRALTREAGLQVELAQLQYALPRLAHSREDLMRQKGGRYGTK
                     GSGEKALELDRRKIEARIRDIQEDLKKVRMERAVQRRRRERAGLPRAAVVGYTNAGKS
                     SLLNAITNADALAEDKLFATLDTTTRRILYAPGRGLLATDTVGFVRNLPHGLVEAFKA
                     TLEEAVLADVLIHVVDASEAEPVRFLETTEKVLGEIGAGDKPTLLAFNKTDLVTDPAR
                     LDLLAERFPGAVFLSVKTGRGIPELLARLEELLTADREEWTVRIPDAEYGLVALLHRR
                     AAVVSESREDDATIVRCRMPEDLREKYGGYRR"
     gene            3631..>4782
                     /locus_tag="P5313_15535"
     CDS             3631..>4782
                     /locus_tag="P5313_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY81827.1"
                     /translation="MPVRLEDISPAVREAEYAVRGAIPTRAQELEAQGRRIIYCNIGN
                     PQSLGQKPLTWLRQVLALCEWPDLADRAPQAFPEDVRRTAREILSGSKHGLGAYSESK
                     GMKFVRQAVAAFIRERDGIEADPEAVYVTDGASKGVQAVLRILIAGPEDGIMIPIPQY
                     PLYSATITLYEGKQVHYYLDEENGWKLNRSMLEESLAEARRYGVRVRAISVINPGNPT
                     GSVLDEENIAMVLDFAREHGLSVLADEVYQSNVYREGDRFVSFAKVLEGKGYKDVSLF
                     SFHSCSKGFLGECGQRGGYLEIRNVPADVTAQLTKLQSISLCANTVGQAAVYLLVRPP
                     RPGEPSRELYVRERDGILAELRSRAELLAEGLNKIPGIRCNPIAGAMYAY"
BASE COUNT          811 a         1654 c         1557 g          760 t
ORIGIN      
        1 cgaaggaggc ggggacctcc ccggcgccgg aactggccgt caaatggccg aacgacctcc
       61 tggtccgggg gcgcaaggcg gcgggcatcc tctgcgagtc ctccgggggg aggtgctgcc
      121 tggggatggg cgtcaactgc ggaactccgt ccggccgggg attccggacg gaggcaaccg
      181 ggctcgcgga ggagctgaag gcgcccgtgg accggttccg ggtcctggaa ctcatcctgg
      241 aacgcctggc cgaggccctg gcggaggaag gctggcggga agaggtttcc cgaagactct
      301 ggctcctgga ccgtcccgcc cggttccgca cggggttggc cgatcccggg gcgccgggga
      361 cgcggatcct ggaagggatc gtgcgcggag tcggcccctc cggggagttg atcctggaaa
      421 ccgcggaggg catccgtgcc ctggcctccg gggaactgtc ccgggactga ggcgggggga
      481 cggaacggct tttcgtacca gggattgacg gcggccggga cagccggtaa ggtttatgcg
      541 tatggccacg aaaagccccg gaccctccgc ggccggaaca ccgtccgaga cgggcatcct
      601 tgcgcgcatc ctttccatct tcacgggagg ggatcccgag gcggagaaga agaaactcct
      661 gcggtccatc ggaaaggatc tttcccggtc gcggtacaaa ttctacaagc ccaagggttc
      721 cgaagccctg ccggggctcg ggcgcttctt ctacgagatc tacaagatcg tggcccccgc
      781 ccagatcctc ctgacaaacg ccgccggttc cggagtcctc cgctccttcg tcatcgagag
      841 cttcctgtcc ccggagcaga aggagatctc ggaacgcctc acggacgcgt ggatccagga
      901 aaaggccaag accctgtcgg tgaaggagct ctccgaactg gtcaagcagg acatgatccg
      961 cttcatctcg gtcttcgacg gggacctgac cgcccggatc gacaccgccc acgcgaccct
     1021 cgtcgccctg gccaacttcg tgaacttcga ctacttcttc ctgctcaaga aattcgattc
     1081 cagcatcccc gagcgcaact tcacctacca ccccaagttc gagacgatct ccgcggacta
     1141 catcgtggac gacctcaagg acttcctgga ggtcttcctg gccctgaacc tggaggcgga
     1201 ctggaaacga atcctgggcg ccctcaagga ataccgcaac atcgacgtcc tgcccgtgga
     1261 tccctggatc aagctgaccg gggccctgaa cgaggtccgc cagagccgca tcctggagca
     1321 ggtcgtccgg cacgcacaga aggatcccta ctggaccgcc gcaccccggt cccaggcgga
     1381 ccggatcgtg gagcccttcc tggagaagct gcgcagccag atggagatcc agctccagaa
     1441 gatcgcccag gaacgccgaa actccaagat cgacgacctg gccaagcagg tcttcggcac
     1501 ctccgtggtg gtccggatga aaaactacac ggacaaggcc aacgtcgtct tcgccaagaa
     1561 gatgatggga ggcttcaccc agaccgctgc cctcaactac atccgggcct tcctgatcga
     1621 cttcttcaaa aaggacgtgc gggagctcgt ggacctcctg atcatccggg gtcagtggac
     1681 caccagcatc ctctcccaac agctttcgga cgcctaccac gccctcctgg agctctccga
     1741 cgagatcatc cgtttcgacg attccctcgg ggacgacggg gacctgggaa acaagcttcg
     1801 gaacgtcctg gccaagagcg accgggacaa ggaacagctc aagtacctgc gcaacctgct
     1861 caaggacacg aacgaccggg cctcgggcat ggtcaaccgc tccgccctga acctgatctc
     1921 cgttggcaag cacctaaaga gcctgatcga ggacctctcc cgcccgcacc acgaggtcgt
     1981 gctcaactgg aaggagatcg agaactccgc cgcccatccc gtcaaggaat ggatgctggg
     2041 cacctacaag aagatctact acctggtcca gctcctgcag ttcttcgtgt cgaaggagca
     2101 gtaaggactc ctgtcacctt ctataccccc cgtacttctc ccgcaggtcc tcgggcatcc
     2161 ggcagcggac gatcgtggcg tcgtcctccc ggctttcgga gaccaccgcg gcccgccggt
     2221 gaagcagggc caccagtccg tactcggcgt cggggatgcg gacggtccac tcctcccggt
     2281 ccgcggtcag gagctcctcc agccgggcca ggagctccgg gatgccccgg ccggtcttga
     2341 cggacaggaa gaccgccccg gggaagcgct ccgcgaggag gtccagccgc gccgggtccg
     2401 tcaccaggtc ggtcttgttg aaggccagga gggtcggctt gtcccccgcc ccgatctccc
     2461 ccaggacctt ctcggtggtc tccaggaaac gtaccggctc ggcctccgag gcgtccacca
     2521 cgtggatcag gacatccgcc aggacggcct cctccagggt cgccttgaag gcctccacga
     2581 ggccgtgggg taggttccgc acgaagccca cggtgtcggt ggccaacagg ccccgtcccg
     2641 gggcgtagag gatgcggcgg gtcgtggtgt ccagggtcgc gaagagcttg tcctccgcca
     2701 gggcgtcggc gttcgtgatc gcgttcagca ggctggactt cccggcgttc gtgtagccca
     2761 ccaccgccgc ccggggcagg cccgcccgct cccggcgcct ccgctggacc gcgcgctcca
     2821 tccggacctt cttgaggtcc tcctggatgt cccggatccg ggcctcgatc ttgcgccggt
     2881 ccagctccag ggccttctcg ccggatccct tggtgccgta gcgcccgccc ttctggcgca
     2941 tcaggtcctc ccgggaatgg gccagccggg gaagggcgta ctggagctgg gccagctcca
     3001 cctggagccc cgcctcccgg gtcaaggccc ggcccgcgaa gatcttcagg atcagttcgc
     3061 tgcggtcgta gacgacccgg cccgagtcct cctcccagtt cctctgctgg accggggaca
     3121 gggggtggtc gaagatgatg gagtccgcgt cctcggcctc ggcggcacgg acgagctcct
     3181 ggaccttccc cgaggacaag aggtgggagg ccgtccggtc ccggatccgc accaggatcc
     3241 gccccgcctc ctggatcccc tgggtcttcg cgaggctcgc gagctccgcc aggaggctct
     3301 ccgcctcggg cttggggatc gagtcggtct gtatccctat gagaagcgcg cgctccgggc
     3361 gcgccgcggt ttcggtcagt tcggtcatgt ccttccttcc ggccgtcccg cgggacggat
     3421 ccgtgccgcg agtatacccg cccgggcccg gattaggtaa gcggtccggg tgtcccggcc
     3481 gcggccgcgg gcggaagggc ggccgggccg cgccccccgc cgcgacgccg cgcggcctcg
     3541 atccttgccc gggggcggaa ggggtggtac aatcctcccg acacgtcccg gaatccccta
     3601 tcgccctcag ccgtgaccca gggaggagcc atgcccgtcc gccttgaaga catcagtccc
     3661 gccgtccggg aagccgaata cgcggtccgc ggcgccatcc ccacccgcgc ccaggagctc
     3721 gaggcccagg gacgtcgcat catctattgt aacatcggga atccccagtc cctgggccag
     3781 aagcccctga cctggctgcg ccaggtcctg gccctctgcg agtggccgga cctggcggat
     3841 cgggctccac aggccttccc cgaggacgtg cgcaggaccg cccgggagat cctttcgggc
     3901 tccaagcacg gactgggggc ctactcggag agcaagggca tgaaattcgt gcgccaggcg
     3961 gtggccgcct tcatccggga gcgggacggg atcgaggcgg accccgaggc ggtctacgtc
     4021 accgacgggg cgtccaaggg ggtccaggcg gtcctgcgca tcctgatcgc cggccccgag
     4081 gacgggatca tgatccccat cccccagtac cccctgtact ccgcgaccat caccctgtac
     4141 gagggcaagc aggtccacta ctacctggac gaggagaacg gctggaagtt gaaccggtcc
     4201 atgctggagg agagcctggc cgaagcccgg cgctacgggg tccgggtccg ggccatctcg
     4261 gtcatcaacc ccgggaaccc cacgggatcg gtcctggacg aggagaacat cgccatggtc
     4321 ctggacttcg cccgggagca cggcctttcc gtcctggccg acgaggtcta ccagagcaac
     4381 gtctaccggg agggggaccg cttcgtgtcc ttcgccaagg tcctggaggg caaggggtac
     4441 aaggacgtat ccctgttcag cttccattcc tgctccaagg gctttttggg ggagtgcggc
     4501 cagcgggggg gctacctgga gatccggaac gtgcccgcgg acgtgacggc ccagctgacc
     4561 aagctccagt ccatcagcct ctgcgccaac accgtgggac aggccgccgt ctacctcctg
     4621 gtccggcctc cccggccggg ggagcccagc cgggagctgt acgtgcggga gcgggacggg
     4681 atcctcgcgg agctgaggtc ccgggcggag cttctggccg agggactcaa caagattccc
     4741 ggcatccgct gcaaccccat cgcgggggcc atgtacgcgt ac
//
LOCUS       DAOTEI010000226         4759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_13020_length_4759_cov_5.519983, whole genome shotgun sequence.
ACCESSION   DAOTEI010000226 DAOTEI010000000
VERSION     DAOTEI010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13020_length_4759_cov_5.519983"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1102
                     /locus_tag="P5313_15540"
     CDS             101..1102
                     /locus_tag="P5313_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY81828.1"
                     /translation="MKRILTLALALLCLGAAVFGQGRYPAKDITITVVFGAGGGTDVL
                     NRLFAGEMRAALGRNVVVANVTGGVSASNGMFHVWNLPHDGYNLAGFSESVVSGPAMG
                     GFDKRMSAWYPFLIAGSPDVLSVPASSPYKTLTDLINAAKAKPGTIKAGAGNVGGLHH
                     LNTLAFEQGTGVKFNYIPYTGSGPTQTAAVTGEIDLVITSIAEQQQLIKAGQLRPLGM
                     LIATPFKVEGFGEIPSSFKFYPDLKKHLPIMQAIGFGVPDDVPADVKKKLDDAFKKTM
                     KSKKVVEFLAANYYEPIGKSGKDAAAIFAKLESLFCWTLWDLGQAKNDPATLGIPKP"
     gene            1520..2293
                     /locus_tag="P5313_15545"
     CDS             1520..2293
                     /locus_tag="P5313_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81829.1"
                     /translation="MLPKERLRRADILFSFLVVLAGAAIVINAMGFPRGAELSGVRND
                     WYVSPALMPYVIGTGLVLMGIALFFTGLKETGTQGLREVLGSIGGLFIRRPAPGAGSL
                     RFLAVTATVVLTVFLYMPRVDFVISAAFLLVALMTMFAFEDDALLRRLLVLYLLLAAP
                     MIAYFASGLGARFEEAFYYASDIPGGLFLVLYILLAARTAGRERRRRFLRLVLIAFAV
                     PLILVPVFKYLLYIPFPREGGVVALMDYVRFDLLDPVQE"
     gene            2297..3847
                     /locus_tag="P5313_15550"
     CDS             2297..3847
                     /locus_tag="P5313_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016400175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY81830.1"
                     /translation="MQFLTLLGHMAASTGSLLISPLAIALLFASVFLGIVFGALPGLT
                     ATLGVALLTTVTYGLDSTLAMVSLLGIYVGAVYGGSYSAVLINVPGTPASAATALDGY
                     PLACRGEAGKALGVTTTASAIGTVVGMIAVIAFSPIITRLALQFTSFEFFLLAFFGIL
                     ISGSLVTPDLVLKGWIAGFLGLFLSLVGRDRLQSFPRFTFGLPQLDSGIDVVPILLGA
                     FGIPQMIGTLKDRIAIKEVQKLGRILPEFRTVLSRFPDIVRSSLIGVGIGAVPGLGED
                     IAAWTSYGAAKKASKHPEEFGKGSYSAIVASETANNSCIGGALIPLYTLGIPGSPPAA
                     MLLGALMLHGLTPGPLLLIENPKFLPETGAILLLAAAAMWVIGIFLAKQVVKVLRIPQ
                     PVLMPIVGLLCIIGSYSLGNNIFNLYLMVPIGIVAYFLGEMGYPIAPLVIGMILGPMA
                     DESLRRALMVSQGSFAPMFTRPVSLILVILIVLTVASQFPFAKKLTDRLAVFTKKAPA
                     ACGDGEGK"
     gene            3963..>4759
                     /locus_tag="P5313_15555"
     CDS             3963..>4759
                     /locus_tag="P5313_15555"
                     /inference="COORDINATES: protein motif:HMM:NF037180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRY81831.1"
                     /translation="MRIEIFFTDPALDGPGSGISEKLSRALGRDLRVRVVEVILPGVP
                     VPRPVAEEAFSDPVVQRVTLDEPAAALLPGWSALVETAWRPGVTDTLALTAREALAIC
                     GGPPESESAGLRTARQYLLYGDAGRPEAEEAARLLHNPLIQEARILTVEDWAAGLRPP
                     ASYPGVSVHDRPDWETFDLESLSDTELEALSRDRLLALTLPELRALRDHFADPRTVEA
                     RRSAGLPAEATDVELEMVAQTWSEHCKHKIFNAEIEYSETDGAGRPSG"
BASE COUNT          739 a         1707 c         1442 g          871 t
ORIGIN      
        1 cgggcaaatt tcattttccg agatttctct tgacatacgg cgagggcgtg gtaagctaat
       61 tttgtattca aaactttcca tacacttcaa ggaggcacct atgaaacgca ttcttacgct
      121 cgccctggct ctcctgtgcc tcggggcggc ggtcttcggc cagggaaggt acccggccaa
      181 ggacatcacc atcaccgtgg tcttcggcgc gggcggcggc acggacgtct tgaaccgcct
      241 gttcgcggga gagatgcggg cggccctggg ccgcaacgtc gtggtggcca acgtcaccgg
      301 cggcgtctcc gcgtccaacg gcatgttcca cgtctggaac ctgccccacg acggttacaa
      361 cctggcgggc ttctccgagt ccgtggtcag cggccccgcg atgggcggct tcgacaagcg
      421 gatgagcgcc tggtatccct tcctgatcgc gggatccccg gacgtgctct ccgtgccggc
      481 gtcctcgccc tacaagaccc tgacggatct catcaacgcc gccaaggcca aaccgggcac
      541 catcaaggcc ggggccggca acgtgggcgg cctgcaccat ctcaacaccc tggccttcga
      601 gcagggcacg ggggtcaagt tcaactacat cccctacacc ggatccggcc cgacccagac
      661 cgccgcggtc accggggaga tcgacctggt gatcacctcc atcgcggaac agcagcagct
      721 gatcaaggcc ggccagctcc ggcccctcgg gatgctcatc gccaccccct tcaaggtgga
      781 aggcttcggc gagatcccct cgtccttcaa gttctatccg gacctcaaga agcacctgcc
      841 catcatgcag gccatcggct tcggcgtacc cgacgacgta cccgcggacg tcaagaagaa
      901 actggacgac gccttcaaga agaccatgaa gtccaagaag gtggtggagt tcctggcggc
      961 caactactac gagcccatcg gcaagtccgg caaggacgcc gcggcgatct tcgccaagct
     1021 cgagtccctg ttctgctgga ccctctggga cctcggacag gccaagaacg accccgcgac
     1081 cctcggcatc cccaagccct gacgattccg ggatacctct ctcctgcggc cggcccctcg
     1141 gggccggcct tttgattaca ggaattgacc acggaggaca cggagctcac ggagagaaag
     1201 agagaaggtg tgcctcattg aatcgacagg attcggaaaa gcaccgcgca agcaaaggcg
     1261 aagaatgccc ttccttacct tccttgttca tcccgaatta ccatatctat cccaaataca
     1321 cattttcctt tcttctccgt gtcctccgtg aactccgtgg tgagtccaga atagcgcgct
     1381 ccgtgccctc ggtggtgagt ccgacaaatc cggtagcttt cccgttgaca agatagggaa
     1441 gtatagtaca ctgttttgta tacaaaatat cgacaacgcc gcgcttcggg cggcgccagc
     1501 ttcgatcccg ggaggaccta tgctgccgaa agaacggctc cgccgagccg acatcctctt
     1561 ctcgttcctg gtcgtgctcg cgggggcggc catcgtgatc aacgcgatgg gcttcccgcg
     1621 tggggcggag ctctccgggg tccggaacga ctggtacgtg tcccccgccc tcatgcccta
     1681 cgtcatcggg acggggctgg tcctcatggg aatagccctc ttcttcacgg ggctcaagga
     1741 aaccgggacc caggggctcc gggaggtcct gggctcgatc ggagggctgt tcattcgccg
     1801 gccggccccc ggggccggct cgctccgctt cctggcggtc acggccacgg tggtcctgac
     1861 cgttttcctc tacatgcccc gggtcgattt cgtgatctcc gcggccttcc tcctcgtcgc
     1921 cctcatgacc atgttcgcct tcgaggacga cgccctgctc aggcggctcc tcgtcctgta
     1981 ccttctcctg gcggcgccga tgatcgccta cttcgcctcc ggcctgggcg cgcggtttga
     2041 agaggcattc tattacgcct cggacatccc gggagggctg ttccttgtcc tgtacatcct
     2101 tctggccgcc cgtacggccg gccgggaacg ccgaaggcga ttcctgcgcc tggtcctgat
     2161 cgccttcgcg gtgcccctga tcctggtccc cgtcttcaag tacctgctgt acataccctt
     2221 cccccgggag ggcggcgtcg tcgccctcat ggattacgta cggttcgacc tgctcgatcc
     2281 ggtccaggag tagaccatgc aattcctaac cctgctgggc cacatggccg cctccacggg
     2341 ctccctgctg atcagccccc tggcgatcgc cctcctgttc gccagcgttt tcctgggcat
     2401 cgtcttcggc gccctcccgg gcctgaccgc caccctgggg gtggccctcc tgaccacggt
     2461 gacctacggc ctggactcca ccctggctat ggtcagcctc ctgggaatct acgtgggcgc
     2521 cgtctacggc ggcagctact cggcggtgct gatcaacgtg ccgggcaccc ccgcctccgc
     2581 ggccacggcc ctggacggct accccctggc ctgtcgcggc gaggccggga aggccctagg
     2641 agtcaccacc accgcctccg ccatcggaac cgtggtcggg atgatcgccg tgatcgcctt
     2701 ctcccccatc atcacccgcc tggccctcca gttcacgagc ttcgagttct tcctgctggc
     2761 cttcttcggc atcctgatct cgggttccct ggtgaccccg gatctggtcc tgaagggctg
     2821 gatcgcgggc ttcctggggc tcttcctctc cctggtgggc cgggaccgcc tccagtcctt
     2881 cccgcgcttc accttcgggt tgccccagct ggactcgggg atcgacgtgg tgcccatcct
     2941 cctgggcgcc ttcgggatcc cccagatgat cggcaccctg aaggaccgta tcgccatcaa
     3001 ggaggtgcag aagctcgggc gcatactgcc ggagttccgc accgtgctga gccgcttccc
     3061 ggacatcgtc cggtccagcc tgatcggggt ggggatcggc gccgtccccg ggctggggga
     3121 ggatatcgcg gcctggacca gctacggggc ggccaagaag gcctccaagc accccgagga
     3181 gttcggcaag ggctcctact cggccatcgt tgccagcgag accgccaaca actcctgcat
     3241 cgggggcgcc ctgatccccc tctacaccct cgggatcccg ggaagtcccc cggcggccat
     3301 gctcctgggg gccctgatgc tccacgggct caccccgggc cccctcctcc tgatcgagaa
     3361 cccgaagttc ctgcccgaga cgggggccat cctccttctg gccgcggcgg ccatgtgggt
     3421 gatcgggatc ttcctggcca agcaggtggt caaggtcctg cgcatccccc agcccgtgtt
     3481 gatgcccatc gtgggcctcc tgtgcatcat cgggtcctac tccctgggga acaacatctt
     3541 caacctctac ctgatggtcc ccatcgggat cgtggcctac ttcctgggcg agatgggata
     3601 ccccatagcc cccctggtca tcgggatgat cctgggtccc atggcggacg agagcctgcg
     3661 ccgagccctc atggtctccc aggggagctt cgcccccatg ttcacccggc cggtatccct
     3721 gatcctcgtc atcctcatcg tcctgaccgt ggcctcccag ttccccttcg ccaagaagct
     3781 cacggaccgg ctggcagtgt tcacgaagaa ggctcccgcg gcctgcgggg acggggaggg
     3841 gaaataggcc cggcggcggg ggccggcccg atggccgccg gctcccctcc cggaagcggg
     3901 ggcccgcctc gtccgatccc cgcttcaaca acgccccggt ttccgttact atccgaccat
     3961 acatgcgcat agagatcttc ttcaccgacc cggccctgga cggccccgga tcggggattt
     4021 cggaaaaact ctcccgggcc ctgggtcggg acctgcgggt acgggtggtg gaggtgatcc
     4081 tgccgggggt tccggtgccc cggccggtcg cggaggaagc cttctcggac cccgtggtcc
     4141 aacgggtgac cctggacgag ccggccgccg ccctccttcc gggctggtcc gccctggtcg
     4201 agacggcctg gaggccgggg gtcacggaca ccctggccct gaccgcccgg gaggccctgg
     4261 ccatctgcgg gggtcctccg gaatccgagt ccgcgggcct gcggaccgcc cggcaatatc
     4321 tgctgtacgg ggacgccgga cgcccggagg ccgaggaggc cgcccggctc ctccacaacc
     4381 ccctcatcca ggaggcccgg atcctcaccg tcgaggactg ggccgccggg ctccgccccc
     4441 cggcctccta ccccggggtc tccgtccacg atcgcccgga ctgggagacc ttcgacctgg
     4501 aatccctctc ggatacggag ctcgaagccc tgtcccggga ccgcctcctg gccctgaccc
     4561 tgccggagct gcgggccctg cgggaccact tcgcggatcc ccggaccgtc gaggcgcgca
     4621 ggtccgcggg cctgcccgcg gaagccacgg acgtggagct cgagatggtc gcccagacct
     4681 ggagcgaaca ctgcaagcac aagatcttca acgccgagat cgagtattcc gagaccgacg
     4741 gggcgggccg tccctcggg
//
LOCUS       DAOTEI010000227         4719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_13171_length_4719_cov_5.384863, whole genome shotgun sequence.
ACCESSION   DAOTEI010000227 DAOTEI010000000
VERSION     DAOTEI010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13171_length_4719_cov_5.384863"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="P5313_15560"
     CDS             complement(<1..915)
                     /locus_tag="P5313_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81832.1"
                     /translation="MTPHKGKATRIAFLSIPPLFLAAYLAGIPVPLPAFLSPARTYLL
                     VPRSGAPSSLAEVKLPGMNLFAVLSDRVVPLKRGAPGLEEIRAESLPARPAVETALRD
                     AEASPKVFGRIRLVCGVHPSAEEVARIREFQERTGRSVSLLVLPDDRPLLRASSYASA
                     SGSSGLKISMTTGPGAREFDLLSAVQDSATLGTWQVPRMPSEGLRFSTAGTGGPVSLV
                     FSGSGGTRREEILLAEPDPGFPRVLVVSRRTGSSGFLDSAYSARRILPEDLETTDLTA
                     YPLVAFDGVPLREIPPGISRTLRSLVERK"
     gene            975..1442
                     /locus_tag="P5313_15565"
     CDS             975..1442
                     /locus_tag="P5313_15565"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRY81833.1"
                     /translation="MDASIINPFIAATTAVFKEMFGLDAAPDKPYVLEETSDSKWEIS
                     GLIGLAGSAQGIVAVRLPSGFAETLLGKTGMTFKTDDEKKQMVGGLVAELTNIVSGNA
                     AGRFKDVNLDIAPPVVVRGDKHQISWPKIAPVLAVPMKTQAGSFELDVCFKGA"
     gene            complement(1519..2898)
                     /locus_tag="P5313_15570"
     CDS             complement(1519..2898)
                     /locus_tag="P5313_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRY81834.1"
                     /translation="MRIPPGSSTPLSRFLSDRRFFTDLFRIAIPIALQNLVASSVNML
                     DTIMVGRLGAVELAAVGLGNQVFFLLLLLLFGIGSGSGVFTSQYWGARDQAGIRRTAG
                     MALALGLSASLVFTAASLAAPRAILGLYSRDPAVLDAGAVYLRIAALSYPALAASFIF
                     SMALRGVERVRLPLAATVISLSANAFLNWVLIFGTLGFPALGVRGAAIATVVSRWMEA
                     AIVLVVTYAKKYPPAGSLRELTDWTRPYVRRFTGIVLPVVFNEAAWALGITFYNGIFA
                     RISTEAIAAFNIQSTISQLSMVLFMGTANASAVMLGKRIGEGNREGAYDWAGRFAVLG
                     PVLGTAIGILLLPTIPALPILFRLEPGVLFQAGAMVCCLAAVFPIKVFNLHIVVGVCR
                     SGGDTRFAAFFDIFGVWLVGVPLAALGAFAWNLSPWAVFLLLCMDEAAKFGLGLWRLR
                     SRKWLRDVT"
     gene            complement(2958..3812)
                     /locus_tag="P5313_15575"
     CDS             complement(2958..3812)
                     /locus_tag="P5313_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY81835.1"
                     /translation="MKTFSKRPSPSERLRGTACPVCGSPETRPLWDLGDFAFARCAGC
                     GHVYQNPRPDPADLTLRYDEEYLEYEVRNALPYLRLMLLGLADLGFERIEAALPEGRS
                     FLDVGCATGALVEHAARRGWEATGVEVCRPAAEYGRRVRGVTILEGSLRDAAFPDDGF
                     DFVHSSHVIEHVPEPGAFLAEIHRILKPGGWCVTVTPNRTSLQAALFGSGWRSVIPDH
                     MHLFSRPGLLRLVRDSGLAPLRTATWGGIALGLAPAPLKAALDRAAKLLGFGDVLAVL
                     ARKPPAGT"
     gene            complement(3809..>4719)
                     /locus_tag="P5313_15580"
     CDS             complement(3809..>4719)
                     /locus_tag="P5313_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY81836.1"
                     /translation="VTEANLEAFLSYCSLRGPEHDESFLPGAGFRPDADHPSFLLLRD
                     GRPAGAASLLLESANRAARRARFAILHAEGGRRGYFALLGASAAAAAPHAGELFLFLP
                     EARAEPLSCLREAGFAVERLSFEMARDDPDLEAPEAPSGYRLEPLRPGDGEGIRAYVE
                     VRNRNFREVLGSLPIDEGTVRADLEDPGIPSGGLAVLRAPDGSACGTMRAERDTDPEC
                     LSIGALSVDREHRGRDLGRFLLRSAAALGRREGFRTLSLSVNALNKNALGLYESEGFT
                     VRGTWVCLSAPVPAVLGRTAPGAGEG"
BASE COUNT          833 a         1559 c         1721 g          606 t
ORIGIN      
        1 cttgcgttcc accaggctcc tgagggtccg ggaaataccc ggggggatct cccgcagggg
       61 caccccgtcg aaggcgacga ggggataggc ggtcaggtcc gtcgtttcca ggtcctccgg
      121 aagtatcctg cgggcggaat acgccgaatc caggaaaccc gatgagccgg ttcggcggct
      181 gacgaccagg acccggggga atccgggatc gggctccgcc aggaggatct cctccctgcg
      241 ggtaccgccg gatccggaaa agaccaggga tacgggtccg cccgtacccg cggtggaaaa
      301 ccggagcccc tcggacggca tccgcggaac ctgccaggtc cccagggtcg cggagtcctg
      361 gaccgcggac aatagatcga attcccgggc acccggcccg gtcgtcatcg agatcttcag
      421 cccggagctg ccggacgccg aagcgtacga ggaagcccgg agcagggggc ggtcgtccgg
      481 gaggacgagg agggagacgg aacgacccgt gcgctcctgg aattcccgga tcctcgccac
      541 ctcctccgcg gaggggtgga ccccgcagac caggcgtatc cgtccgaaca ccttcgggga
      601 ggcttccgcg tcccgaaggg cggtctccac ggcgggccgg gccggcaggg attccgcccg
      661 gatctcttcg aggcccggcg ccccccgttt cagggggacg acccggtcgg agagcacggc
      721 gaagagattc ataccgggaa gcttgacctc cgcgagggag gagggggcgc cggaacgggg
      781 aacgagcagg taggtgcgcg cgggcgaaag gaacgcaggc aggggaacgg ggatccccgc
      841 gaggtacgcc gccaggaaga gcggaggaat cgaaaggaag gcgatccggg tcgcttttcc
      901 cttgtgcggg gtcatggttc cgcctattct ataagcgagc ccgccgattc cgcaaggcgg
      961 aaaaaggagc ccccatggac gcgtccatca tcaacccctt catcgccgcg accacggccg
     1021 tcttcaagga gatgttcggc ctggatgccg cgcccgacaa gccctacgtc ctggaggaga
     1081 cctcggactc gaagtgggag atctccggcc tgatcggcct ggcgggatcc gcccagggga
     1141 tcgtggcggt ccgcctgccc tcgggattcg cggaaaccct gctgggcaag accggcatga
     1201 ccttcaagac cgacgacgaa aagaaacaga tggtgggcgg cctggtggcg gagctcacga
     1261 acatcgtgtc cggcaatgcc gccgggcggt tcaaggacgt caacctggac atcgccccgc
     1321 ccgtggtcgt ccggggcgac aagcaccaga tatcctggcc caagatcgcc ccggtcctgg
     1381 ccgtgcccat gaagacccag gcgggctcct tcgaactgga cgtgtgcttc aagggggcgt
     1441 aatggatccg ggaattcgct gaagcgcagc ttcagcgccg tgatttcctg tccgaaaact
     1501 atattttccg acaggaaatc acgtcacgtc cctgagccat ttccgggacc gcagcctcca
     1561 gagacccagg ccgaacttgg ccgcctcgtc catgcagagg aggaggaaca cggcccaggg
     1621 cgagaggttc cacgcgaagg cgcccagggc ggccaagggc acccccacga gccagacccc
     1681 gaagatgtcg aagaaggccg cgaaccgggt gtccccgccg gacctgcaca cccccaccac
     1741 gatgtggagg ttgaagacct tgatcgggaa gaccgcggcc agacagcaga ccatcgcgcc
     1801 ggcctggaac aggaccccgg gctccagccg gaacaggatg ggcagcgccg ggatcgtcgg
     1861 cagcagcagg attccgatcg cggtgcccag gacggggccg agcacggcga agcggcctgc
     1921 ccagtcgtag gccccctccc ggttgccttc cccgatccgt tttcccagca ttaccgccga
     1981 ggcgttcgcg gtgcccatga acaggaccat ggacagctgg gagatcgtgc tctggatgtt
     2041 gaacgccgcg atggcctccg tggagatccg cgcgaagatc ccgttgtaga aggtgatgcc
     2101 cagggcccag gccgcctcgt tgaagaccac gggaaggacg atcccggtga acctccggac
     2161 gtagggccgg gtccagtccg tcagctcccg gagggatccc gcggggggat actttttcgc
     2221 gtaggtgacg accaggacga tggcggcctc catccagcgg gagacgacgg tggctatggc
     2281 cgccccccgc acccccaggg ccgggaatcc cagggtcccg aagatgagga cccagttcag
     2341 gaaggcgttg gcggagaggg agattacggt cgccgcaagg gggagccgga cccgttccac
     2401 cccccggaga gccatggaga agatgaagct ggcggccagg gcggggtagc tcagcgctgc
     2461 gatgcggaga tagacggccc ccgcgtccag gaccgcgggg tcccgggaat acagacccag
     2521 gatggcccgg ggggccgcca gggacgcggc cgtgaagacc agggacgccg agagtccgag
     2581 ggccagggcc atgcccgcgg tcctccgtat ccccgcctgg tcccgggctc cccagtactg
     2641 ggaggtgaac accccggagc cggatccgat cccgaacaag aggagcagga ggaggaagaa
     2701 gacctggttc cccaggccca ccgccgcgag ctccacggct cccaggcggc ccaccatgat
     2761 cgtgtccagc atgttcaccg aggacgccac gaggttctgg agggctatcg ggatggcgat
     2821 gcggaagagg tccgtgaaga atctccgatc cgagaggaac cgggatagcg gggtggatga
     2881 gcctgggggg attcgcacgg ggtgcatcct agccgaaggg ggaaccggtg ggcaaggccg
     2941 gggtcccggg ccgtcggcta ggttccggcg ggcggtttcc gggccagcac cgccaggaca
     3001 tccccgaagc cgagtagctt ggctgcccga tccagggcgg ccttgagggg tgccggggcc
     3061 aggcccaggg cgatccctcc ccaggtcgcg gtccggaggg gcgcgagtcc cgaatcccgg
     3121 accaaccgga ggagaccggg gcgcgagaac aggtgcatgt ggtccgggat gacgctgcgc
     3181 catccggagc cgaagagggc ggcctgcagg ctggtccggt tcggggtcac cgtgacgcac
     3241 cagcctccgg gtttcaggat ccggtggatc tccgccagga aggccccggg ctcgggtacg
     3301 tgctcgatga cgtggctgga atgcacgaag tcgaagccgt cgtccgggaa ggccgcgtcc
     3361 cggaggctgc cctccaggat cgtgacgccc cgaactctcc gtccgtactc cgccgcggga
     3421 cggcagacct cgacccccgt cgcctcccag ccccgccgcg ccgcgtgctc caccagggct
     3481 cccgtggcgc agccgacatc caggaagctc cggccttcgg gaagggcggc ctcgatcctc
     3541 tcgaagccca ggtccgcaag gcccaggagc atgaggcgga ggtagggaag ggcgttccga
     3601 acctcgtact cgaggtattc ctcgtcgtag cgcagagtca ggtccgcggg atccgggcgg
     3661 gggttctggt agacgtggcc gcagccggca cagcgcgcga aggcgaagtc cccgaggtcc
     3721 cagagggggc gggtttccgg ggagccgcag accggacagg ccgtcccccg gagccgttcc
     3781 gagggggagg ggcgcttgga aaaggtcttc atccctcccc ggcccccggg gccgtgcggc
     3841 ccaggacggc cgggacgggc gcggaaaggc agacccaggt gccccggacc gtgaatccct
     3901 cggactcgta gagccccagg gcgttcttgt tcagcgcgtt gaccgaaagg gacagagtcc
     3961 ggaagccctc ccggcggccc agggccgcgg cggagcggag caggaaccgt ccgaggtccc
     4021 ggcctcggtg ttcccggtcc accgacaggg cgccgatgga caggcattcg gggtccgtgt
     4081 cccgctccgc ccgcatcgtg ccgcaggccg agccgtccgg ggcgcggagg accgccagac
     4141 ccccggaggg gatccccgga tcctccagat cggcccggac ggtcccctcg tcgatcggaa
     4201 gggagcccag gacttcccgg aagttgcggt tccggacctc cacgtaggcc cggatgccct
     4261 ccccgtcccc cgggcgcagg ggttccagcc ggtaccccga gggagcctcc ggggcctcga
     4321 ggtccgggtc gtcccgggcc atctcgaagg acaggcgctc gacggcgaag cccgcctccc
     4381 gcaggcagga aaggggttcg gcgcgggctt ccgggaggaa gaggaagagc tctccggcgt
     4441 ggggcgccgc ggcggccgcg gaggcgccca gaagcgcgaa gtacccccgc cgcccgccct
     4501 cggcgtgcag gatcgcgaac cgggcccgcc gggcggcccg gttcgcggac tccaggagca
     4561 gggaggctgc gcccgcgggc ctgccgtccc ggagcaggag gaaggagggg tggtcggcgt
     4621 caggccggaa gcccgccccc gggaggaagc tttcgtcgtg ttcgggaccc cggagggaac
     4681 agtaggagag gaaggcctcc aggttcgcct cggtgaccg
//
LOCUS       DAOTEI010000228         4703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_13235_length_4703_cov_4.882745, whole genome shotgun sequence.
ACCESSION   DAOTEI010000228 DAOTEI010000000
VERSION     DAOTEI010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_4703_cov_4.882745"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="P5313_15585"
     CDS             <1..1426
                     /locus_tag="P5313_15585"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRY81837.1"
                     /translation="HMPPGAEEERAEQLAWLEGSAHEKAVDPRIGEHLAALGCSDSNP
                     GGDPSLDPDARAFLRVLYRTYAREVKLPADLVTETAKAQSLGQSAWVRARSENDFSAF
                     RPHLERILDLKRRAAACLDPAKKPYDVLLDLFEPGSDEAQVRRVFDPLRKDLSSLLAR
                     IRSRPQVDDSFLRKPCPKDAQTAASRYFMELLGYDLDRGRLDVTAHPFTTTVGRADVR
                     ITTRYDEGYFLSSLFSTIHETGHALYEQGVNPPAEYAGTCLADGASMGIHESQSRMWE
                     NVVGRSRPFWSREYPALRAVLGEVLSGVSLDAFLRGINKVEPSLIRTEADEVTYGLHI
                     ILRFEMEADLLSGRLAVQDVPAAWNAKTRDLLGIEPPSDAKGCLQDVHWSAGLFGYFP
                     SYSLGNLYAAQFVQALRKDLDLDGRLREGDYASVLGWLRKHIHAPGAALLPNELCERV
                     TGKPLDAAHFVDYLNRKYAEIYGF"
     gene            1416..2201
                     /locus_tag="P5313_15590"
     CDS             1416..2201
                     /locus_tag="P5313_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81838.1"
                     /translation="MDSELAAAGAGWAAAALGGLSLGAAAAFCLLALSAGRSGRGGKP
                     RRVLSGVSLGLAAAVACLAALLILPPKPLLADRTLWIWAGAWGALGCLSAFFPGRAGI
                     PLAVLVLSFLGLAAGETSVWHPLVPGREVARLVPYEATAAGASGDLAVPDRNAVPVLS
                     RVRVEGSDCALEARVLDLAGPLAPLFGPRRYRLSRLTGGGGGGLLDFPRKPGPLTGVL
                     EGREFSVGWVSLRTVRSPALPLEALRALSWSFDGEGELEARLP"
     gene            complement(2218..3756)
                     /locus_tag="P5313_15595"
     CDS             complement(2218..3756)
                     /locus_tag="P5313_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81839.1"
                     /translation="MKDPGAVSKGTREQALRYIDYASLQERFDALVRGGSDLKTRRKF
                     ITDVFLAKNRAIRDLDFRTLDPRSIEDDYLRPRKDLSSFVLRNWVNGVFLPSLEPDLT
                     GGLFLFGLGRMFSAYNDVGVQSCTDADLNFVIQDEVPPAGRKVLETELRKLRKEAFDQ
                     FGILIEIHPEYTIQDASAVSASLTHPDPERRLGSALFYKSNEKSIFVFKDHPELRARV
                     FSKVSALPDSCLFEHFLGLGSAKNSFLKIRMDRTPLTVLEEYTCEQIQTRAVIGSRAF
                     GQYCFKTLRKVPFASPPVWYFSMKYFVNRVYDYVCAMRNMGYGLPQIGFDSEDSTGSP
                     DPDYLFLRNAHKLMLYLQELIQLSMESFGMECDYSYISRARFLKFMEIDGDKFRKNFQ
                     ELVLGGKLLPPTAREKFLRLKRKIEEKSRDRIVHCDKKDLEAFPEGFKYETIDRDSAR
                     RRIWVPYSWADLGFFVFNVIAARIGQIVDSRLVPPLARLDARAGAPEPEAGSPARKRA
                     RRSA"
     gene            complement(3949..>4703)
                     /locus_tag="P5313_15600"
     CDS             complement(3949..>4703)
                     /locus_tag="P5313_15600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRY81840.1"
                     /translation="LIVSAARPSGLPLKEVLALRFRMGWKNFFLSWLLFPALALLTLG
                     ISLLLPGVRYDPSMSQLIERLTKGMSPEKVAELREMAGAAPPLLLTLVQGLAAGISIN
                     AVFGFGEEAGWRGFLVKELRGSSFWGASVFTGAVWGLWHAPLILQGHNYPEHRVLGVF
                     LMILFCVLLSPVFLYIRLRSKSAVAAAVAHGSMNATAGLSIMPLAGGNDLTVGMTGLA
                     GFVAAAGLLAALYLLDRRAKEPLMASRLGEDA"
BASE COUNT          721 a         1606 c         1638 g          738 t
ORIGIN      
        1 ccacatgccc cccggggcgg aggaggaacg cgcggagcag ctggcctggc tggagggatc
       61 ggcccacgag aaggccgtcg acccccggat cggcgaacac ctggccgccc tgggctgctc
      121 ggactccaac cccggggggg acccgtccct ggatcccgac gcccgcgcct tcctgcgggt
      181 cctgtaccgg acctacgccc gggaggtgaa gctccccgcg gacctcgtca ccgagaccgc
      241 gaaggcccag agcctcggcc agtccgcctg ggtccgggcc cgctccgaga acgacttttc
      301 cgccttccgg ccccacctgg agcgcatcct ggacctcaag cgccgggccg cggcctgcct
      361 ggacccggcc aagaagccct acgacgtact cctggacctc ttcgagcccg ggagcgacga
      421 ggcgcaggtc cgccgggtct tcgaccccct gcggaaggac ctctcctccc tgctggcccg
      481 gatccggtcc aggccccagg tggacgactc cttcctgcgt aaaccctgcc ccaaggacgc
      541 ccagaccgcg gccagccggt acttcatgga gctcctgggc tacgacctcg accgcggccg
      601 gctggacgtc accgcccatc ccttcaccac caccgtgggc cgggcggacg tccgcatcac
      661 cacccgctac gacgaggggt acttcctctc cagcctcttc tccacgatcc acgagaccgg
      721 ccacgccctc tacgagcagg gggtgaaccc cccggcggag tacgcgggga cctgcctggc
      781 cgacggggcg tccatgggga tccacgagtc ccagtcccgg atgtgggaga acgtggtcgg
      841 ccgcagccgc cccttctgga gccgcgagta tcccgccctg cgggccgtcc tgggggaggt
      901 cctgtccggg gtctccctgg acgccttcct gcgggggatc aacaaggtgg agccctccct
      961 gatccggacc gaggcggacg aggtcaccta cgggctccat atcatcctgc gcttcgagat
     1021 ggaggcggac ctcctttcgg gccgcctggc cgtccaggac gtcccggcgg catggaacgc
     1081 caagacccgg gacctcctgg ggatcgagcc gcccagcgac gccaagggct gcctccagga
     1141 cgtccactgg tccgcggggc tcttcggcta cttccccagc tattccctgg gcaacctcta
     1201 cgccgcccag ttcgtccagg ccctgcggaa ggacctggac ctggacggcc gcctccggga
     1261 gggagactac gcctcggtgc tcggctggct taggaagcac atccacgcgc ccggggcggc
     1321 cctgctcccg aacgagctct gcgagcgggt gacgggcaag cccctggacg ccgctcattt
     1381 cgtggactac ctgaaccgca agtacgcgga gatctatgga ttctgagctg gccgccgcgg
     1441 gcgccggctg ggccgctgcg gccctcgggg ggctttccct gggggcggcg gcggccttct
     1501 gcctgctcgc cctgagcgcc ggccggtcag gccggggggg caaaccccga cgggttctca
     1561 gcggggtttc cctggggctc gcggcggcgg tcgcctgcct ggcggcgctc ctcatccttc
     1621 cgcccaaacc cctgctcgcg gaccggacgc tctggatctg ggcgggcgcc tggggggccc
     1681 tgggatgcct gtccgcgttc ttcccgggcc gggccggaat tcccctggcc gtgctggtcc
     1741 tctccttcct gggcctggcc gcgggggaga cctcggtctg gcaccccttg gtgcccggcc
     1801 gggaagtcgc ccgcctggtg ccctacgagg cgaccgcggc gggggcttcc ggcgacctgg
     1861 ccgtgccgga ccggaacgcc gtgcccgtgc tgtcccgggt gcgggtggag ggatcggact
     1921 gcgccctgga agcccgggtc ctggacctgg ccggccctct cgcccccctc ttcggccctc
     1981 ggaggtatcg gctctcgcgg ctgaccggag gcggcggggg cggactgctg gacttccccc
     2041 ggaagccggg gcccctgacg ggggtgctgg aagggaggga gttctccgtc ggctgggttt
     2101 ccctgcggac ggtccggagt cccgccctgc ccctggaggc gctgcgagcc ctgtcctggt
     2161 ccttcgacgg ggagggggaa ctcgaggccc ggttgcccta gcaggtccga caggctccta
     2221 ggccgaacgc cgggcgcgtt tccgggcggg acttccggcc tcgggctccg gggcgccggc
     2281 ccgggcgtcc aagcgcgcga ggggcggcac cagccgggag tccacgatct ggccgatccg
     2341 ggcggcgatc acgttgaaca cgaagaatcc caggtccgcc caggaatagg gcacccagat
     2401 cctgcggcga gcggagtccc ggtcgatcgt ctcgtacttg aagccctccg ggaaggcttc
     2461 caggtccttc ttgtcgcagt gcacgatccg gtcccgggac ttctcctcga tcttgcgctt
     2521 gaggcgcagg aacttttccc gggctgtcgg aggcaggagc ttccctccca gcacgagttc
     2581 ctggaaattc ttgcggaact tgtccccgtc gatctccatg aacttcagga acctcgcccg
     2641 ggagatgtag gagtagtcgc actccatccc gaaggactcc atggacagct ggatgagctc
     2701 ctggagatag agcatgagct tgtgggcgtt gcgcaggaag aggtagtccg gatcggggct
     2761 tcccgtggaa tcctcggagt cgaagccgat ctgcgggagc ccgtacccca tgttccgcat
     2821 ggcgcagacg tagtcgtaga cccggttgac gaagtacttc atggagaagt accagaccgg
     2881 cggggaggcg aaggggacct tccggagggt cttgaagcag tactggccga aggcccggga
     2941 tccgatgacg gcccgggtct ggatctgctc gcaggtgtat tcctccagga ccgtgagggg
     3001 ggtccggtcc atccggatct tcaggaagga gttcttggcg ctcccgagac ccaggaagtg
     3061 ctcgaagagg caggagtccg gcaaggcgct caccttggaa aacacccgcg cccggagctc
     3121 cgggtggtcc ttgaatacga agatgctctt ctcgttgctc ttgtagaaga gggcgcttcc
     3181 gagacgccgc tccggatccg gatgggtcag ggaggcggag acggcgctcg cgtcctggat
     3241 ggtgtattcc gggtggatct cgatgaggat gccgaactgg tcgaaggctt ccttccggag
     3301 tttccggagc tccgtctcta ggaccttccg tccggccggg ggcacctcgt cctggatcac
     3361 gaaattcagg tccgcgtcgg tacaggactg gacccccacg tcgttgtacg ccgagaacat
     3421 ccgtcccagg ccgaagagga agagtccccc cgtcaggtcc ggctccaggg atgggaggaa
     3481 gaccccgttc acccagttcc gcagtacgaa ggagctcaag tccttccggg gccggaggta
     3541 gtcgtcctcg atggaccggg gatccagggt gcggaaatcc aggtcccgga tggcccggtt
     3601 cttggccagg aagacgtcgg tgatgaattt ccggcgggtc ttcaggtccg agccgccgcg
     3661 caccagggcg tcgaagcgtt cctgcaggga ggcgtagtcg atatagcgca gggcctgctc
     3721 gcgggttccc ttggaaacgg cccctggatc cttcatgctt cggcggcctc ggcggttaag
     3781 gagtacgccc ggtccgggac cgggcgtcgt tccattgtag caattatatc taaattgtca
     3841 acattgccgg atgtcggatg caggcatcct gcatcgccgc acggtggtac ggcccggagc
     3901 agcggccccg tccgcccggt tcggcgcggc ccgggctccg ggggcggact acgcgtcctc
     3961 cccgagccgg gaagccatca gcggctcctt cgcccttcgg tccaggaggt acagcgccgc
     4021 cagcaggccg gcggccgcga cgaatcccgc caggcccgtc atgccgacgg tcaggtcgtt
     4081 gccgccggcc aggggcatga tggaaaggcc cgccgtggcg ttcatgctcc cgtgggcgac
     4141 ggcggcggcc acggcggact tcgagcggag gcggatgtac aggaagaccg gggacagcag
     4201 gacgcagaac aggatcatca ggaagacccc gaggacccgg tgctcggggt aattgtgccc
     4261 ctgcaggatg agcggcgcgt gccagagacc ccagacggcg ccggtgaaga ccgatgcgcc
     4321 ccagaaggag gaaccccgga gttccttcac caggaagccc ctccacccgg cctcctcccc
     4381 gaacccgaag acggcgttga tcgagatgcc cgccgcgagg ccctggacca gggtcaggag
     4441 gaggggcggc gcggccccgg ccatctcccg gagctccgcg accttctcgg ggctcatgcc
     4501 cttggtcagc cgttcgatca gctgggacat ggaagggtca tagcggaccc cgggcaggag
     4561 gagggagatg cccagggtca gcagggccag ggccgggaag aggagccagg aaaggaagaa
     4621 attcttccag cccatccgga accgcagggc caggacctcc ttcaggggaa ggccggaagg
     4681 ccgcgccgcc gatacgatca acg
//
LOCUS       DAOTEI010000229         4679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_13329_length_4679_cov_3.738106, whole genome shotgun sequence.
ACCESSION   DAOTEI010000229 DAOTEI010000000
VERSION     DAOTEI010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13329_length_4679_cov_3.738106"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5313_15605"
     CDS             <1..205
                     /locus_tag="P5313_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81841.1"
                     /translation="SKGAPVVPVLSFSEIDVEPFPLPSEVRWVREKEGAWEVGFSVRL
                     PMPAGPAERDFFRIHEYYRDSAR"
     gene            382..2316
                     /locus_tag="P5313_15610"
     CDS             382..2316
                     /locus_tag="P5313_15610"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY81842.1"
                     /translation="MSIPSIEQILESPLPIKLIVMLGMSLVFLYVRKRDTEGFTSGYF
                     YLFALQAVRDLMFAVFPVPQIYLVSDLFVFAALTYLAAASLGVRALAAGVSVVNLAAA
                     ILCLVDYSMPFLPSLPFSPFSLVPALDAAVLAILLAVRGRSLDRALMNAGTVAAGFGA
                     AYAAAAFFLGYEHSLLHKLAVPLFYFWPLGTAFYQLETHEGQLVRALEYYEGAVDSIY
                     NLSVETGSAMRKSFAISDMLDGMARVVVAETDADGGAVLLVDEFEDVVSVRSVQGRFP
                     PPFKLPEDLPRKQERVDAFVRHAQFKLGETLFGEAAKTGKPIFAPQAEEDGRIFTNGP
                     EDFLRIGSLMAVPLLVEDTVIGVLAVERLPARDPFTEGQFDHLKTLANFGALQISNFF
                     SFQEARERSERERAANIAAEIQGLIVPKKLPDFPAASLGAFTAPAIGVSGDYFDILQT
                     RKDRLLLAVGDVAGKGVAAALVMVMVRSILHLVANTDKDASTMLSWINRGITGKIEMD
                     HYATLGLVDVNLATGEVEYANAGHQPLLIYRAQEDGIESVELKSIPIGVERGMNYARK
                     PLKLRAGDVLLLYTDGVVEAMNAQGRQFGRKSLGSSLLRCRELSARETALKIRSDLAN
                     FVGDTRQHDDQTVLVLKMKL"
     gene            2364..2702
                     /locus_tag="P5313_15615"
     CDS             2364..2702
                     /locus_tag="P5313_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521480.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HRY81843.1"
                     /translation="MELKIRKTGTNYIIDVNGEMDLYNSYKLKELVMKMLEKKVERFI
                     INLENVDYIDSSGIGALIYICSTIKKQGDKLAITNIHGSVKKVIELTKLMGYFPIMNS
                     IEEAILRMEE"
     gene            2706..3269
                     /locus_tag="P5313_15620"
     CDS             2706..3269
                     /locus_tag="P5313_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY81844.1"
                     /translation="MDSADTTYLSGIHEIRVDENSPLFEKTNMLYKEFPSDYRQIRYF
                     TLLIVQSAPLEIKEINLLEQQISEIIKNAVKHGNKCAPEKKVRVWYEFDASHAHLIVQ
                     DEGEGFKDLETWNDFNRRRLEKLAAQDFEGLADFVSFRTDDSDDNDGGNALFAALEYW
                     NGGFVFNAKRNAVAMLKKFPQKKHAIG"
     gene            3509..>4679
                     /locus_tag="P5313_15625"
     CDS             3509..>4679
                     /locus_tag="P5313_15625"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRY81845.1"
                     /translation="MRKPAGIKGVPLSRLAFVAALAMASASCTASYFIVGPEGFSGLA
                     TDLSRRIEGAGGLVPARSADRADLVLYLHGYWSGRVPGGTGSPVLSREAWVPTREART
                     GAGGGSAWTTSGARLEECLSGTVPIMPLERVAPPLLAMPVDGLRIGKAGYPLWREAAA
                     AAVPAGTRGRRAMPALDALLSAMEAEALQAAERVSGSLVWIAAMGDLMPGRGIDARLL
                     ADGPSSVFSAEVLRVLGEADLAIANLEGALTLRGRAAVKTFTFRSPPAVAEALSAAGL
                     DAFLLANNHALDWGATGLYDTLEALTGAGLGYAGAGRTIEDAARPFLATVKGRPVAVW
                     GAAAFPREGNGWDGAAAAAGPDSPGIFWLDAPGMARLASGFREDVLDLVMIHAGTE"
BASE COUNT          788 a         1546 c         1535 g          810 t
ORIGIN      
        1 ttccaagggg gcgcccgtcg tgccggtcct ctccttctcc gagatcgacg tggagccctt
       61 tcccctgccc tccgaagtcc gttgggtccg ggaaaaggag ggagcctggg aggtcgggtt
      121 ctcggtgcgg ctacccatgc ccgcgggtcc cgcggagagg gattttttcc ggatccacga
      181 atactaccgg gactccgccc ggtaggcggc cgtccttcga aacccctcaa accgcgcgga
      241 accggcaccg acaactatgg tagaacggtc ctgatttggc ttgaccccca tcttcccggg
      301 tgatagactt ttttggactt ttcatcgggc gatcccccgg atccgtcccg gagtccgccg
      361 agtgatttcc gagggttcgc aatgagcatt ccgtcgatcg agcagattct cgagtccccg
      421 ctgcccatca agctcatcgt gatgctcggg atgagcctcg tgttcctgta cgtgcgcaag
      481 cgcgatacgg aagggttcac ctcggggtat ttctacctct tcgcactcca ggcggtccgg
      541 gacctgatgt tcgcggtatt cccggtcccc cagatctacc tcgtttccga cctcttcgtg
      601 ttcgcggccc tgacctacct ggccgctgcc tccctgggag tccgggccct ggccgcgggg
      661 gtttccgtgg tgaacctcgc ggccgcgatc ctctgcctcg tcgactattc catgcccttc
      721 ctgccctccc tcccgttctc acccttcagc ctggtcccgg ccctggacgc ggcggtcctg
      781 gcgatcctcc tggcggtccg aggccggagc ctggatcggg ccttgatgaa cgccgggacc
      841 gtcgccgcgg gcttcggcgc ggcctacgcc gccgcggcct tcttcctggg atacgagcac
      901 agcctgctgc acaagctggc ggtgcccctc ttctatttct ggcccctcgg tacggcgttc
      961 taccagctgg agacccatga aggacagctc gtccgcgccc tggagtacta cgagggggcg
     1021 gtggattcca tctacaacct ctccgtggag accggatccg ccatgcggaa atccttcgcc
     1081 atctcggaca tgctggacgg catggcccgg gtggtcgtcg cggaaacgga cgcggacggg
     1141 ggagcggtcc tgttggtgga cgagttcgag gacgtcgtga gcgtccggtc cgtccagggg
     1201 cgtttccctc cccccttcaa gctgcccgaa gacctgcccc ggaagcagga gagggtggac
     1261 gccttcgtcc ggcacgccca gttcaagctc ggggagaccc tgttcggcga ggccgccaag
     1321 accgggaagc cgatcttcgc accccaggcg gaagaggacg gacggatttt cacgaacggc
     1381 ccggaggact tcctgcggat cggatccctg atggccgtcc ccctcctcgt ggaggatacg
     1441 gtcatcggcg tcctggccgt ggagaggctt ccggcccggg atcccttcac ggagggacag
     1501 ttcgaccacc tcaagacact ggccaacttc ggggccctgc agatcagcaa cttcttctcc
     1561 ttccaggaag cccgggaacg gagcgagcgc gaacgggccg cgaacatcgc cgcggagatc
     1621 cagggactaa tcgtacccaa gaagctgccg gacttccccg ccgcgtccct gggggccttc
     1681 acggccccgg ccatcggcgt cagcggcgac tacttcgaca tcctgcagac ccggaaggac
     1741 cgcctgctcc tggccgtcgg tgacgtggcg ggcaaggggg tcgccgcggc cctggtcatg
     1801 gtcatggtgc ggtccatcct gcacctcgtg gcgaacacgg acaaggacgc ctccaccatg
     1861 ctatcctgga tcaaccgggg aattaccggc aagatcgaga tggatcatta tgccaccctg
     1921 ggcctggtgg acgtgaacct cgcgacgggt gaggtggagt acgcgaacgc gggacaccag
     1981 cccctcctga tctaccgcgc ccaggaggac ggcatagagt ccgtggaatt gaagagcatt
     2041 ccgatcggcg tggagcgggg catgaactac gcccggaagc ccctcaagct ccgggccggg
     2101 gacgtcctgc tcctgtacac cgacggggtg gtggaggcca tgaacgccca gggccgacag
     2161 ttcggccgga agagcctggg gtcttccctg ctgcggtgcc gcgaactgtc cgcccgggag
     2221 acggccctca agatccgatc cgacctggcg aacttcgtgg gggacacgcg ccagcacgac
     2281 gaccagaccg tgctcgtcct gaagatgaag ctctaggggg ccgaggaaat gcccgatctc
     2341 ttgctaagga gggcctgggt accatggaat tgaagatccg caagacgggt accaactaca
     2401 tcatcgacgt caacggcgag atggacttgt acaactcgta caagctcaag gagctggtga
     2461 tgaagatgct ggagaagaag gttgagcgtt tcatcatcaa cctggagaac gtcgactaca
     2521 tcgattccag cgggatcggc gccctcatct atatctgctc caccatcaag aagcagggcg
     2581 acaagctggc gatcacgaac atccacggtt cggtgaagaa ggtcatcgaa ctcaccaagc
     2641 tcatgggtta tttccccatc atgaacagca tcgaggaagc catcctccgg atggaggaat
     2701 gatccatgga ttccgcggac accacctacc tctcgggcat ccatgagatc cgggtcgacg
     2761 agaacagccc cctgttcgag aagaccaaca tgctgtacaa ggaatttccg tccgactacc
     2821 ggcagatccg gtatttcacc ctcctgatcg tccagtcggc ccccctggag atcaaggaga
     2881 tcaacctgct ggagcagcag atctccgaga tcatcaagaa cgccgtcaag cacgggaaca
     2941 agtgcgcccc ggagaaaaag gtccgggtct ggtacgagtt cgacgcctcc cacgcccacc
     3001 tgatcgtcca ggacgagggg gagggcttca aggacctgga gacctggaac gacttcaacc
     3061 gccgcaggct ggagaaactg gcggcccagg acttcgaggg gctcgcggat ttcgtctcct
     3121 tccggaccga cgactccgac gacaacgacg gcggcaacgc cctgttcgcg gccctggagt
     3181 actggaacgg cggatttgtc ttcaacgcca agcggaacgc ggtggccatg ctgaagaagt
     3241 tcccgcagaa gaagcacgcc atcggctgaa agccggcgaa tcgcggcgtt tcgaaagcct
     3301 ccgcccggac ttacatacaa aagtatgctc gtccggtcgg gggctttcgc gccttgcgct
     3361 tcatccggct ttcagccgat gcattgaacc ggatgagcgg cgaatcgcgg cgttgcgtgg
     3421 atgcgccgtc ggcgcaccgg tcgggggctt tcgcgcctca tccggcagcg ggctcgccgc
     3481 ccggcggcac cgtggagggg cgcgtgaaat gaggaaacct gccgggatca aaggcgttcc
     3541 gctctctcgc ctggcgttcg tcgcggcgct cgcgatggcc tcggcttcct gcaccgcaag
     3601 ttatttcatc gtcggcccgg aagggttctc cggcctcgcc acggaccttt cccggaggat
     3661 cgagggtgct ggcggcctgg ttccggctcg ctccgcggac cgcgcggacc tggtgctcta
     3721 cctccatggg tactggtcgg ggcgcgttcc cggcggcacc ggctctcccg tgctgtcccg
     3781 cgaagcctgg gtcccgacgc gcgaggcccg gacgggagcc gggggcggca gtgcctggac
     3841 aacctcaggc gcccgcctgg aagagtgcct gtcggggacg gtccccatca tgccgctgga
     3901 gcgggtggcg ccgcccctgc tcgcgatgcc cgtggacggg ctcaggatcg gcaaggcagg
     3961 ctatccgctc tggcgcgagg cggccgccgc agcggtcccc gcaggaaccc gcggacgccg
     4021 cgcgatgccc gccctggacg ccctgctctc cgccatggag gcggaggccc tgcaagccgc
     4081 cgaacgggta tccgggagcc tggtgtggat tgccgcgatg ggggacctga tgcccgggcg
     4141 gggcatcgac gcgcggcttc tggcggacgg cccgtcctcc gtgttctccg cggaggtcct
     4201 gcgcgtcctg ggggaagcgg acctggcgat cgcgaacctg gaaggcgcgc ttaccctccg
     4261 gggcagggcg gccgtcaaga ccttcacctt ccgttccccg cccgccgtgg cggaagccct
     4321 ctccgcggcc gggctggacg cgttcctcct ggccaacaac cacgcgctgg attggggagc
     4381 gacgggactg tacgacaccc tggaagcgct cacgggcgcc ggactgggct atgcgggggc
     4441 tggacggacc atcgaggacg ccgcccggcc cttcctcgcg accgtgaagg gccggcccgt
     4501 ggccgtatgg ggagcggccg cgttcccccg ggagggcaac ggatgggacg gcgccgcggc
     4561 cgcggccggg ccggattcgc ctggcatttt ctggctcgac gcgccgggga tggccaggct
     4621 cgcctcgggg ttcagggaag atgtcctgga cctggtcatg atccatgccg ggaccgagt
//
LOCUS       DAOTEI010000230         4621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_13552_length_4621_cov_4.649146, whole genome shotgun sequence.
ACCESSION   DAOTEI010000230 DAOTEI010000000
VERSION     DAOTEI010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13552_length_4621_cov_4.649146"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="P5313_15630"
     CDS             <1..531
                     /locus_tag="P5313_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81846.1"
                     /translation="KKALVGADLGIPDPNYTGAYYWTQKDGGQIVFWAYLLAKPGQGF
                     AQRAQQLKERIRASELTNMKIDRGYFGEDEYETVKRLIENQRILELETVEGFLGTLRF
                     WWASASTDYFFGYLDNMKKVGYREISAFLDRYIHKNLEIVAVRVHPSVYEAEKKAFAD
                     AGYETLGADNAFWWKK"
     gene            549..2069
                     /locus_tag="P5313_15635"
     CDS             549..2069
                     /locus_tag="P5313_15635"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY81847.1"
                     /translation="MTSNKRSPARPRAFLGAALSAALLLAVAAGCVSTPPASSGSGGE
                     APGSGSPAPVPTAKAAPSPVKTEPGPFYKSNIGDFETRTLSNGIKLYVKKNRTNRVLN
                     LKLVLLGGSSVVPRAKAGLDDMTLSLLSRGSASYPYEKILDVEYRTSSSISHSSGYDY
                     SSYDLNCIDTYLPELLDIYLDGFLRPSLLADRFRDVRNEAAQARESRMGDPDQRARYL
                     GTQAIFAGHPYASDPAGTPESLDSVTLEDVKAHHGTLLNADRIFLAAVGNFDVDALAA
                     KLESALGSIPRKGSATPVSAPPATSGPPILEAHPASEGVAYIQGYFRIPGRQDRDYVP
                     FALAADMLDDLMFNVVREKYGACYSVGAIFRPAAASYGILWIYQASDIEGIRKYVKEA
                     ADYLAQGKLIRSKDASGQYVLGSIEDRLPAYRSKYVNAVFANQRTNSDIGAQVVRSVV
                     YAGDPADYLRTPDRIAAVTPADIRRVYKTWFLDQSFRWVIVSDEAGLKRVPAGEYR"
     gene            complement(2159..4246)
                     /locus_tag="P5313_15640"
     CDS             complement(2159..4246)
                     /locus_tag="P5313_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRY81848.1"
                     /translation="MKELEDLRKAVSAWEAEDRAKVETAVEAASIAHEGQVRASGEPA
                     VTHPLRVALILAELGLDSDAVVAAVLHHAPDEDGPARADLAERFGPVAALLVEETSRI
                     AALKAKNKTLQAAETIRKMLLAMTRDIRVILVKLADKLDNMRTLRWLPEEERKRIAAE
                     CLDIFAPLADRLGLSAVKDELEDLALKELNREAFDQIKAIVADKKGERAEFLARVEKD
                     IRTAAAQEKLFVEVSSRAKHFYSIYQKMRKRNKSADELYDLLGIRLLCGTENDCYALL
                     GVVHRLWKPLEGRFKDYIAMPKANGYRSLHTTVMAYEGRLLEIQIRTYDMHRVAEFGV
                     ASHWLYKKGRSRDTVRTEDLPLVNKLKDWSGMLSSGAEFLQEIKRELLKESIFVFTPK
                     GDVVELPAGSTPLDFAYEVHTAVGNRCVGAKADGAIVPLTYELRNTQVVEIMTSNTAR
                     PNIDWLRAVKTSKAKSKIRQWLIVNGQALVIDRNVVARKKADAPEAKGEARPRGEGRP
                     EPGKAGETAPEARPAVPEPDRPLGQIEIHPYVPRAVLSSDKAGISVSGGRGLMIRFAG
                     CCRPTTGDPIVGYVSRGRGIIVHRRDCRNLPFLSDFKERAIEVVWETRHPGLTRRFRI
                     TAKRIPDLFGEVETAVKKIQGHLIEGKLEEDGDRLVGWFTVRMEKPEDGRKIERSVRG
                     VPAILKVQVVSGD"
     gene            complement(4243..>4621)
                     /locus_tag="P5313_15645"
     CDS             complement(4243..>4621)
                     /locus_tag="P5313_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81849.1"
                     /translation="ARFVVLAGVSGPFDLITANPPYVASAETDRLQGRPGPGHGGTAG
                     EDGVRREPRAALDGGPDGLDFYRRLVPEAEKALALGGFLVVEIGEDQGASVGALFRGC
                     GFTGVEVMRDLGGRDRVVRGMRG"
BASE COUNT          726 a         1635 c         1493 g          767 t
ORIGIN      
        1 aagaaggctc tggtcggggc ggacctgggc atcccagatc cgaactacac gggggcgtac
       61 tactggaccc agaaggacgg aggccagatc gtcttctggg cctacctcct ggccaagccc
      121 ggacagggct tcgcccagag ggcgcagcag ctcaaggaga ggatccgggc ctcggaactg
      181 acgaatatga agatcgaccg gggatacttc ggcgaggacg agtacgagac cgtcaagcgt
      241 ctgatcgaga accagcggat cctggagctc gagaccgtgg agggcttcct gggaaccctc
      301 cggttctggt gggcctcggc ctccacggac tatttcttcg ggtacctcga caacatgaag
      361 aaggtcgggt accgggagat atccgccttc ctggaccggt acatccacaa gaacctcgag
      421 atcgtggccg tccgggtcca tcccagcgtc tacgaggccg aaaagaaggc cttcgccgac
      481 gcggggtacg agaccctggg ggcggacaac gccttctggt ggaaaaaatg atgcgaggac
      541 ccaagccgat gacatcgaac aagcgttccc cggcccgccc gcgggccttc ctcggagccg
      601 ccctctcagc ggccctcctc ctggccgtcg cggccggctg cgtgtccacc cccccggcct
      661 cctcgggttc gggcggcgag gccccgggtt ccggctcccc ggcgcccgtg ccgaccgcca
      721 aggccgcccc ttccccggtc aagacggagc cgggcccctt ctacaagtcc aacatcgggg
      781 acttcgagac ccggaccctg tccaacggca tcaagctcta cgtgaagaag aaccggacga
      841 accgggtcct gaacctcaag ctggtcctgc tgggaggctc ttcggttgtg ccccgggcca
      901 aggccgggct ggacgacatg accctgtccc tcctttcccg gggctcggcg tcctacccct
      961 acgagaagat cctggacgtc gagtaccgga cctcctcctc catctcccat tcctcggggt
     1021 acgactactc cagctacgac ctcaactgca tcgacaccta cctgcccgaa ctcctggaca
     1081 tctacctgga cggattcctc cgcccgtccc tccttgccga ccggttccgg gacgtgagga
     1141 acgaggcggc ccaggcccgg gaatcccgca tgggggaccc ggaccagcgg gcccgctacc
     1201 tggggaccca ggccatcttc gcgggccatc cctacgcctc ggatcccgcg ggcaccccgg
     1261 agtccctgga ttccgtcacc ctggaggacg tcaaggccca ccacggaacc ctcctgaacg
     1321 ccgaccggat cttcctggcc gcggtgggga acttcgacgt ggacgccctt gcggccaagc
     1381 tggaatcggc cctggggtcg atcccgagga agggctccgc gacccccgtc tcggctcctc
     1441 cggcgacctc gggccccccc atcctggaag cccatccggc ctccgagggc gtggcctaca
     1501 tccagggcta cttccgcatc cccggccggc aggaccggga ctacgtgccc ttcgccctgg
     1561 ccgcggacat gctggacgac ttgatgttca acgtggtccg ggagaagtac ggggcctgct
     1621 attccgtggg ggcgatcttc cggcccgcgg cggcctccta cgggatcctg tggatctacc
     1681 aggcctcgga catcgagggg atccggaagt acgtgaagga ggccgcggac tacctggcgc
     1741 agggaaagct gatccggtcc aaggatgctt cgggacagta cgtcctgggc tccatcgagg
     1801 accggctccc cgcctaccgg agcaagtacg tgaacgccgt cttcgccaac cagcggacca
     1861 actcggacat cggcgcccag gtcgtgcgga gcgtcgtgta cgccggggat cccgcggact
     1921 acctgcggac cccggaccgg atcgccgccg tcacccccgc ggacatccgc agggtctaca
     1981 agacctggtt cctggaccag agcttccgct gggtgatcgt cagcgacgag gcggggctga
     2041 agcgggtgcc ggcgggggag tataggtgaa taagtgaata ggtgaattag tacataggac
     2101 tggctggatt gcggtccgcg gatccttcga ttcacctatt cacgattcac atattcactc
     2161 aatcaccgct caccacctgc accttcaata tcgcggggac gccccggacg gagcgctcga
     2221 tcttgcggcc gtcctcgggc ttctccatgc gcaccgtgaa ccagcccacc aggcggtccc
     2281 cgtcctcctc gagcttcccc tcgatcaggt ggccctggat cttcttgacc gcggtctcca
     2341 cctccccgaa gaggtcgggg atgcgcttgg ccgtgatccg gaagcgccgg gtcaggccgg
     2401 ggtgccgggt ctcccagacc acctcgatgg cccgctcctt gaagtccgaa aggaaaggca
     2461 ggttccggca gtcccggcgg tgcacgatga tcccccgtcc ccgggagacg tagcccacaa
     2521 tgggatcccc cgtggtgggg cggcagcacc cggcgaagcg gatcatgagg ccccggcccc
     2581 cggacacgga gatccccgcc ttgtcgctgg agaggaccgc cctcggcacg taggggtgga
     2641 tctcgatctg gcccaggggg cggtcgggct ccgggacggc gggacgggct tcgggggcgg
     2701 tctccccggc cttgccgggc tccgggcggc cctccccccg gggccgggcc tcgcccttgg
     2761 cctccggcgc gtcggccttc ttccgggcca ccacattgcg gtcgatcacc agggcctggc
     2821 cgttgacgat gagccactgc cggatcttgg acttggcctt ggaagtcttc accgcccgca
     2881 gccagtcgat gttgggccgg gccgtgttgg aggtcatgat ctccacgacc tgggtgtttc
     2941 ggagctcgta ggtgaggggc acgatggccc cgtcggcctt ggctccgacg cagcggtttc
     3001 ccacggcggt gtggacctcg taggcgaagt ccaggggggt ggagcccgcg ggaagctcca
     3061 cgacgtcccc cttgggggtg aagacgaaga tggactcctt gaggagctcc cgcttgatct
     3121 cctggaggaa ctccgccccg gaggagagca tcccgctcca gtccttgagc ttgttcacca
     3181 ggggcaggtc ctcggtccga accgtgtccc gggatcggcc cttcttgtag agccaatggg
     3241 aggccacgcc gaactccgcc acccggtgca tgtcgtaggt gcggatctgg atctccaaaa
     3301 gccggccctc gtaggccatg accgtcgtgt ggaggctacg gtacccgttg gccttgggca
     3361 tggcgatgta gtccttgaac cttccctcca ggggtttcca gagccggtgc accaccccca
     3421 ggagggcgta gcagtcgttc tcggtaccgc agagcagtcg gatgcccaaa aggtcgtaca
     3481 gctcgtcggc gctcttgttg cgcttccgca tcttctggta gatcgagtag aagtgcttgg
     3541 cccgggagct gacctccacg aaaagctttt cctgggccgc ggccgtccgg atgtccttct
     3601 ccacccgcgc caggaactcg gcccgctcgc ccttcttgtc cgccacgatc gccttgatct
     3661 ggtcgaaggc ctcccggttc agctccttga gggccaggtc ctccagctcg tccttgacgg
     3721 ccgagaggcc cagccggtcc gccagggggg cgaagatgtc caggcattcc gcggcgatgc
     3781 gcttgcgctc ctcctcgggc agccagcgca gggtgcgcat gttgtccagc ttgtccgcca
     3841 gcttcaccag gatcacccgg atgtcccggg tcatggccag gagcatcttt cggatggtct
     3901 ccgcggcctg gagggtcttg ttcttggcct tgagggcggc gatgcgggag gtctcctcca
     3961 ccaggagggc cgcgacgggt ccgaatcgct ccgccaggtc cgcccgggcc gggccgtcct
     4021 cgtccggggc gtggtggagc accgcggcga ccacggcgtc cgagtccagg cccagctccg
     4081 ccaggatgag ggccacccgc agggggtggg tcaccgcggg ttccccggag gcccggacct
     4141 gcccctcgtg ggcgatcgag gccgcctcca cggcggtctc caccttggcg cggtcctccg
     4201 cctcccaggc ggaaacggcc tttcgcagat cctccagctc cttcatcccc gcatcccccg
     4261 gaccacccgg tcccggcctc ccaggtcccg catcacctcg acccccgtga acccgcagcc
     4321 gcggaacagg gcccccaccg aggccccctg gtcctcccct atctctacaa ccaggaagcc
     4381 cccgagggca agagcttttt ccgcttccgg aaccaggcgg cggtaaaagt ccagcccgtc
     4441 cggtccgccg tccagggccg cccggggttc cctacggacc ccgtcctccc ccgccgtccc
     4501 gccgtgcccc ggaccggggc ggccctggag gcgatccgtc tcggcgctcg caacgtaggg
     4561 gggattcgcg gtgatcaggt cgaagggccc ggagaccccg gccaggacga cgaaacgggc
     4621 g
//
LOCUS       DAOTEI010000231         4543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_13874_length_4543_cov_3.835784, whole genome shotgun sequence.
ACCESSION   DAOTEI010000231 DAOTEI010000000
VERSION     DAOTEI010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13874_length_4543_cov_3.835784"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="P5313_15650"
     CDS             <1..1177
                     /locus_tag="P5313_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81850.1"
                     /translation="LSVLALGACSRFSGSVREVRRESLFSLGYGTLEDQLDLFRVEGM
                     APPAKTRIAMQDGIFFLANGNGAKVLTLSSFGDLLSMVYNPDRNPAPIVLSPSESQAS
                     GRFARPYPFLSPGEIAVDSRKHVYVEDRLPPDRRAFDAAANTSLEYVVLRFSTDGKFI
                     DFLGQEGIGGTPFPVISGIYATAQDDCVVLCLTREAWLVYWFDARGILTSTVRIRRDS
                     LPLPDDRNLVPSLERIYPDPDGNGIYLKVDYYTESIDPATKTRSGIVYDRAFVYRMDP
                     RTAAYTDRWEILPYEPPRGRGEDEAPPRIWEFLGAARGRNLFFTTTGEDGKTFVAVYN
                     GPAKSMKRYSITIEPEELQYTTLYLSPGGILCALMGTRFEARVVWWRFDRILSGGAL"
     gene            1138..2670
                     /locus_tag="P5313_15655"
     CDS             1138..2670
                     /locus_tag="P5313_15655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRY81851.1"
                     /translation="MALRPDPLGRRPLKPLVAAARAADLDRRIREEAGVPEAALMENA
                     AAGMEAVLVSRGLAGGTVTALVGKGNNGGDALAVLRRLAFRSAGGRRDGLSAVLSSGE
                     PGGLAGQQLKAARACGTRVLSWDREEPACRELLARSSLILDGIAGTGLSGPLRPREAA
                     LAEAAGSCGAVVAAVDVPSGCGDGIPPGSPVLRADLTLCVEPYKVCLFRPKARPFAGE
                     IVPVSGVFPRDSGEESPIRLLEPGDLADLVPPLRPEAHKYQRGHCLVFAGEVGTSGAA
                     VLAARGATAGGAGLVTLHVRPDIWQAAASSLAGELVRPLPPDPADLDLGRARAVVAGP
                     GWGRDGIAAAVLRRLWDSPLPLVLDADALTLLRDLDLPARVYPLVLTPHPGEAERLFG
                     RPAADFLRSPEETLPDLCRRWGAVVVLKTPVTWIASPDGRFAVHDGMDGSLAVGGSGD
                     VLAGLCGALLARGLGPFEAARAAVLAHGLAGTEARKALGHYGAEALPGFAAKVLGGIH
                     GQ"
     gene            2660..3208
                     /locus_tag="P5313_15660"
     CDS             2660..3208
                     /locus_tag="P5313_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81852.1"
                     /translation="MVNKGPEMRIPDDGGVSEDGIRRHYSRERRLAGASPEVRWLSRQ
                     YGAKRPGILGSLLATGPLRFMFFTLLLFMAAGALLSFLSGREDSGVLGGSLYKAAAFR
                     FGDSAYITVTRTARNEAAYQGPLRLAAYLDGRPAASGDFTAGAVPKEEYRLTAPSPEP
                     VRKLSVRVEAGGLALELTAPVK"
     gene            3305..3715
                     /locus_tag="P5313_15665"
     CDS             3305..3715
                     /locus_tag="P5313_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81853.1"
                     /translation="MIQFYFLSVLLNVLGGYALLIQGGGDKGTKFDGVRNFLKDMTVR
                     LVLGVLAVIVGFFKLLTVMRGDIPIVGDFLPALAGLASGFSLLLEFYRSNSNVTTDTL
                     ERMDRIFTANRRIVGISSMIVGFVHFLFASVLFL"
     gene            3721..4098
                     /locus_tag="P5313_15670"
     CDS             3721..4098
                     /locus_tag="P5313_15670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRY81854.1"
                     /translation="MPDSVAGIALRNGLVFVARRRPGGSMGGKWEFPGGKLEPGETPE
                     ETLVREFREELGLEVRPGALLGESEFENEGVRFRLRAYRVEFDGEPSFLAEHQDVRWL
                     SRTELEDLDLAPSDRSLLRFLPG"
     gene            complement(4140..>4543)
                     /locus_tag="P5313_15675"
     CDS             complement(4140..>4543)
                     /locus_tag="P5313_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81855.1"
                     /translation="AAVTAQLAALRPDQVQSLFKTGNGYTAFVDKAYNVVNDHPDILP
                     RIFDAAELKRDCELSKDLVPILDQVNQLADSLRDTQMAVNSDAIAGALEVYQAVKLIK
                     DRVQGLNVVAEEMGEFFKRSRKPAGAPARAS"
BASE COUNT          597 a         1645 c         1568 g          733 t
ORIGIN      
        1 cctttccgtc ctggccctgg gagcctgttc ccggttctcc ggctccgtcc gggaggtccg
       61 gcgggaatcc ctcttttccc tggggtacgg caccctggag gaccagctgg acctcttccg
      121 ggtggagggg atggcgcctc ccgccaagac ccggatcgcc atgcaggacg ggatcttctt
      181 cctggccaac gggaacgggg ccaaggtcct caccctgtct tccttcgggg acctgttgtc
      241 catggtctac aacccggacc ggaatccggc gcccatcgtc ctctccccgt cggaatccca
      301 ggcctccggc cgcttcgccc gcccctatcc cttcctgtcc cccggggaga tcgccgtgga
      361 ctcccgcaag cacgtctacg tggaggaccg cctacccccg gaccgccggg ccttcgacgc
      421 cgccgcgaac acctccctgg agtacgtggt gctccgcttc tccacggacg ggaagttcat
      481 agacttcctg ggccaggaag ggatcggagg cacccccttc ccggtcatct cggggatcta
      541 cgccaccgcc caggacgact gcgtggtgct ctgcctgacc cgggaggcct ggctggtcta
      601 ctggttcgac gcccggggca tcctgacctc caccgtgagg atccgccggg attccctgcc
      661 cctgccggac gaccggaacc tggtgccctc cctggagcgg atctacccgg accccgacgg
      721 gaacgggatc tacctgaagg tcgactacta caccgagtcc atcgaccccg ccaccaagac
      781 ccgctccggg atcgtgtacg accgggcctt cgtataccgg atggaccccc ggaccgcggc
      841 ctacacggac cgctgggaga tcctgcccta cgagcccccc cggggccggg gggaggacga
      901 ggctcctccg cggatctggg agttcctggg cgccgcccga ggaaggaacc tcttcttcac
      961 caccaccggc gaggacggga agaccttcgt ggccgtctac aacgggcccg ccaagtccat
     1021 gaaacgctac tccatcacca tcgagcccga ggaactccag tacacgaccc tctacctctc
     1081 cccgggcggc atcctctgcg ccctcatggg cacccgcttc gaggcccggg tggtctggtg
     1141 gcgcttcgac cggatcctct cgggaggcgc cctctgaaac ccctggtggc cgcggcccgg
     1201 gccgcggacc tggaccggcg gatccgggag gaggccgggg ttcccgaggc ggccctgatg
     1261 gaaaacgccg cggccgggat ggaagccgtc ctggtatcgc gggggctcgc gggcggaacc
     1321 gtgacggccc tggtgggcaa ggggaacaac ggaggggacg ccctggccgt cctccggcgc
     1381 ctggccttca ggtccgccgg cggccggagg gacggtcttt cggccgtcct ctcctccggc
     1441 gagcccgggg gactcgccgg gcagcagctc aaggcggccc gggcctgcgg gacccgggtt
     1501 ctctcctggg acagggaaga gcccgcctgc cgggaactcc tcgcacggtc ctccctgatc
     1561 ctggacggta tcgcgggtac gggcctgtcc ggccccctcc ggccccggga agccgccctg
     1621 gcggaggcgg ccggatcctg cggcgccgtg gtcgcggccg tggacgtgcc ctccggatgc
     1681 ggggacggga tcccgccggg atcccccgta ctccgggcgg acctgaccct ctgcgtcgag
     1741 ccctacaaag tctgcttgtt ccggcccaag gcccggccct tcgcgggcga gatcgttccg
     1801 gtctccgggg tcttcccccg ggattccggg gaggagtccc cgatccgcct cctggagccc
     1861 ggcgacctcg ccgacctggt ccccccgctc cgtccggagg cccacaagta ccagcggggg
     1921 cattgcctgg tcttcgccgg ggaagtcggc acttccggtg ccgccgtcct ggccgcccgg
     1981 ggagccaccg cgggtggggc ggggctcgtg accctccatg tccgcccgga catctggcag
     2041 gccgccgctt cctccctggc cggggagctc gtgcggcccc ttccaccgga ccccgcggac
     2101 ctggacctgg gcagggcccg cgccgtcgtg gccgggcccg gctggggccg ggacggaatc
     2161 gcggcggccg tcctccggag gctctgggac tcgcccctgc ccctggtcct ggacgcggac
     2221 gccctgaccc tcctccggga cctggacctc ccggcccgcg tgtatcctct ggtcctgacc
     2281 ccccaccccg gagaggccga gcgcctgttc gggcgcccgg cggcggactt cctgcggtcc
     2341 ccggaggaaa ccctgccgga cctgtgccgc cgatggggcg ccgtggtcgt cctgaaaacc
     2401 ccggtcacct ggatcgcgtc cccggacggc cgcttcgcgg tccacgacgg gatggacggg
     2461 tccctggccg tgggcggaag cggggacgtc ctggccgggc tttgcggcgc cctgctggcc
     2521 cgcgggctgg gccccttcga ggcggcccgg gcggctgtcc tggcccacgg cctagccggg
     2581 accgaggccc ggaaggccct gggacactac ggggcggagg ccctgcccgg tttcgcggcg
     2641 aaggtcctgg gaggaatcca tggtcaataa gggacccgag atgaggattc cggacgacgg
     2701 gggcgtctcg gaggacggga tccggcgcca ctacagccgg gagcgccggc tggccggcgc
     2761 atcccccgag gttcggtggc tgtcccggca gtacggggcc aagcgcccgg gcatcctggg
     2821 ctccctcctg gccacgggcc ccctccggtt catgttcttc accctccttc tgttcatggc
     2881 cgcgggagcc ctgctctcgt tcctttcggg tcgggaggat tccggcgtcc tggggggcag
     2941 cctctacaag gccgcggcct tccggttcgg ggattccgcg tacatcaccg tcacccggac
     3001 cgcccggaac gaggccgcct accaagggcc cctgcgcctg gcggcctacc tggacggccg
     3061 gcccgcggcc tccggggact tcacggcggg agccgtcccc aaggaggagt accggctcac
     3121 ggctccctcc cccgagcccg tgcggaagct ttccgtccgg gtcgaggccg gggggctcgc
     3181 cctcgagctg acggctccgg tcaagtaggg acctgtccgg accgccgttc cgacttacct
     3241 tgaccggacc cgggctttgg gttagattga acgggatgaa gaaccacggc ccgggggttg
     3301 ccgcatgatc caattctatt tcctcagcgt cctgctgaac gtcctggggg gctacgccct
     3361 cctgatccag ggcggcgggg acaagggaac aaaattcgac ggagtgcgca acttcctcaa
     3421 ggacatgacc gtccgcctgg tcctgggcgt cctggcggtc atcgtgggct tcttcaagct
     3481 cctcacggtc atgcgggggg acatccccat cgtgggggac ttcctcccgg ctctggcggg
     3541 cctggcctcc gggttctcgc tcctcctgga gttctaccgc agcaattcca acgtgaccac
     3601 ggacaccctg gagcgcatgg accggatctt caccgcgaac cgcaggatcg tgggcatatc
     3661 ctccatgatc gtcggcttcg tccacttcct cttcgcctcg gtcctcttcc tctagccccc
     3721 atgcccgact ccgtggcggg gatcgccctc cggaacggcc tcgtcttcgt cgcccggcgc
     3781 aggcccggcg ggtcgatggg cggcaagtgg gagttccccg gggggaagct cgagcccggg
     3841 gagaccccgg aggagaccct ggtccgcgag ttccgggagg aactgggcct ggaggttcgg
     3901 ccgggcgccc tgctggggga atccgagttc gagaacgagg gagtccgctt ccgcctgcgg
     3961 gcctaccggg tcgagttcga cggcgagccc tccttcctgg cggagcacca ggacgtccgc
     4021 tggctgtcac ggaccgagct cgaggacctg gatttggcgc ccagcgaccg gagcctgcta
     4081 cggtttctgc cggggtaggg cggcggggcg ccgaagagcc gccgggctcc gggaaatcgt
     4141 tacgaagccc gggcgggggc cccggcgggc ttccgggacc gcttgaagaa ctcgcccatc
     4201 tcctcggcca cgacgttgag gccctggacc cgatccttga tcagcttgac cgcctggtag
     4261 acctccaggg ccccggctat ggcgtcggag ttgaccgcca tctgggtgtc ccggagggag
     4321 tccgctagct ggttaacctg gtccagtatg gggacgaggt ccttggagag ctcgcagtcc
     4381 cgcttgagct ccgcggcgtc gaagatccgg ggcaggatgt ccggatggtc gttgaccacg
     4441 ttgtacgcct tgtccacgaa ggccgtgtag ccgttgcccg tcttgaacag ggactgcacc
     4501 tggtcgggcc ggagggcggc aagctgggcg gtgacggcag caa
//
LOCUS       DAOTEI010000232         4464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_14229_length_4464_cov_4.561352, whole genome shotgun sequence.
ACCESSION   DAOTEI010000232 DAOTEI010000000
VERSION     DAOTEI010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14229_length_4464_cov_4.561352"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="P5313_15680"
     CDS             <1..510
                     /locus_tag="P5313_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRY81856.1"
                     /translation="DSLRSSYEAALPRLTELGITAFRQGQDERGRLLDAFKADAASVL
                     FATDSFWEGVDAPGDTLRMVVITKLPFRVPTDPVQQARAEAIERREGNPFLELSLPEA
                     VLRFKQGFGRLIRHSEDRGVVAVLDSRIVHKRYGSQFLESLPRCRVETGPLERIRGEI
                     ARFFSVDAP"
     gene            566..1792
                     /gene="manA"
                     /locus_tag="P5313_15685"
     CDS             566..1792
                     /gene="manA"
                     /locus_tag="P5313_15685"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HRY81857.1"
                     /translation="MPVLRLENPIQHYDWGTPDAIPALLGMDEPDGKPCAELWMGAHP
                     KAPSVAETEDGRVPLDRLIARDPVSALGAPTVERFGPALPFLFKVLSAARPLSIQAHP
                     QKLKAERGFEKENAEAVPLDAPDRNYRDPNHKPEMLVALDDFEGLCGFRPIPEILRLI
                     RTVAPREFERIAGNLVRNPGKVELAVLFYRFISYSANLKRDLLYYTERRIRRILDHEP
                     DPAVRDALSWVLTLMRLFPGDVGALAPLILNHFRLTPGQALFIEPGQIHAYLRGTGLE
                     IMANSDNVLRGALTSKNIDIPELLSVLSFVPEGVRIYGPRETGTAVEEYASEAAEFRL
                     DRIRLEPGRDVPRGRPGPEILLCLEGWITISDTSGGPGRGGLALERGGCAFITADVRD
                     YSLAGSGLAFRAGVTA"
     gene            1789..2373
                     /locus_tag="P5313_15690"
     CDS             1789..2373
                     /locus_tag="P5313_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HRY81858.1"
                     /translation="MTIWADADSLPAPVREILARRARPAGRTGPDGSGTAEASTGVRV
                     VFAAGRPIPVPRGSEFLLVETGGPDAVDARIEVLAAPGDLVVTRDIPFAARLAEAGLA
                     VINDRGDEWTADTVRERLSRRDFMAGLRSSGLAEMNRARTWGPREKKAFADTLDRVVR
                     RIQATGPRSQDPGLASDVGGGAGRSAADGSVPGS"
     gene            complement(2295..3311)
                     /locus_tag="P5313_15695"
     CDS             complement(2295..3311)
                     /locus_tag="P5313_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRY81859.1"
                     /translation="MDTIPLALILFGAPVERSLGRACAAALVSRGAGPETPRSAGTSG
                     KRGRTRADAGGPEPARVPVPPVSPPPSPSRARILKARAALGRVLRTAGRGIALAHVWY
                     VLTVALLLLVYRSVDPPVTVLMAYRAGVDGWKLAPQRNVKLAKVPVLARRMVVSVEDG
                     KFYSHNGVDFEAILYARQVNRQVGRPLYGGSTLSMQTARTLFLVPFKSYLRKYLELIA
                     TVEMELILPKQRILEIYLSWAEWGKGVFGIEAAARRFYGTSVSRLDVDQTARLVALLS
                     SPIRYTPETLHKNGILRTRYQYLAGKYGEKEEPGVGTQDPGTDPSAADRPAPPPTSEA
                     SPGS"
     gene            3540..>4464
                     /locus_tag="P5313_15700"
     CDS             3540..>4464
                     /locus_tag="P5313_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY81860.1"
                     /translation="MKKVRFALLVALVLSSTFLYAEATGVPGLHRFVLDNGLELFVLE
                     NPSAPLAYIEIAIRGGGVAQTPETAGVFHFYEHMMFKGNSKYRTAAEVSSAYLDLGVP
                     SWNGSTGSEYVDYYFTVPSDRLREGLEFWSYAIREPLLDPGEIESEKGVVVSEITGNY
                     SEPRSIYAAGIDRRLFPKYPWRRDPGGTVEAIRGVTAEKLRTMQRTYYIPNNAALFVG
                     GDVRADAVHQLVKEVYGSWERGPDPWAKPQDPQAFPAVRKTAFVVYPDPTVNPRFGIA
                     QIYYRGPDVGRDPVATYAADVWGYLVENPSGR"
BASE COUNT          672 a         1559 c         1525 g          708 t
ORIGIN      
        1 gactccctgc gctcctccta cgaggcagcc ctgccccgcc tgaccgagct cgggatcacg
       61 gccttccggc agggccagga cgagcggggg cgcctcctgg acgccttcaa ggccgacgcg
      121 gccagcgtcc tcttcgccac ggactccttc tgggaggggg tcgacgcccc cggggacacc
      181 ctccgcatgg tcgtgatcac gaagctcccc ttccgggtgc ccacggaccc cgtccagcag
      241 gcccgggccg aagccatcga gcgccgggag ggcaacccct tcctggagct ctccctcccc
      301 gaagccgtgc tccgattcaa gcaaggcttc ggacgcctca tccggcactc cgaggaccgg
      361 ggggttgtgg cggtcctgga ctcccggatc gtccacaaac ggtacggcag ccagttcctg
      421 gagtccctgc cccgctgccg ggtggagacc ggccccctgg agcggatccg cggcgagatc
      481 gcccggtttt tctccgtcga tgcaccttga gtcccccttc atccttcact attcactatt
      541 cactattcct tgctaaactc ttcccatgcc cgtcctccgc ctggaaaacc caattcagca
      601 ctacgactgg ggaaccccgg atgctattcc ggccctcctc ggaatggacg agcccgacgg
      661 gaagccctgc gcggagctct ggatgggagc ccatcccaag gccccctcgg tggcggagac
      721 ggaggacggc cgggtgcccc tggacaggct catcgcccgg gatcccgtct ccgccctggg
      781 cgccccgacg gtggagcgat tcggccccgc cctccccttc ctgttcaagg tcctctcggc
      841 ggcgcggccc ctgtccatcc aggcccatcc ccagaagctc aaggccgagc gggggttcga
      901 gaaggagaac gccgaggcgg tgcccctgga cgccccggac aggaactacc gggacccgaa
      961 ccacaagccc gagatgctgg tcgccctgga cgatttcgag ggtctctgcg gcttccggcc
     1021 catcccggag atcctgcgcc tgatccggac ggtcgccccc cgggagttcg agcggatcgc
     1081 cggcaacctg gtccggaatc ccggcaaggt ggagctcgcg gtgttgttct accgcttcat
     1141 ctcctactcg gcgaacctga agcgggacct cctgtactat acggaacggc ggatccggcg
     1201 gatcctggac catgagccgg atccggccgt acgggacgcc ctgtcctggg tcctgaccct
     1261 catgcggctc ttcccggggg acgtgggagc cctggcgccc ctcatcctga atcatttccg
     1321 cctgaccccg ggccaggccc tcttcattga gccgggacaa atccacgctt acctgcgcgg
     1381 aacgggcctc gagatcatgg ccaactcgga caacgtcctt cgtggagccc tgacctccaa
     1441 gaacatcgac atcccggaac tcctgtccgt cctgtccttc gttccggagg gagtccggat
     1501 ctacggccct cgggaaaccg gaaccgcggt cgaggaatat gcctccgagg ccgcggagtt
     1561 ccgcctggac cgcatccgcc tcgaacccgg ccgggacgtc ccgcgcggac ggcccgggcc
     1621 ggagatcctg ctctgcctgg aggggtggat cacgatatcc gatacgtccg gcggccccgg
     1681 gcgggggggc ttggccctgg agcgaggcgg ctgcgccttc atcaccgccg acgtccggga
     1741 ctactccctc gcggggagcg gcctggcgtt ccgtgcgggg gtcacggcgt gaccatctgg
     1801 gccgacgcgg actccctgcc cgcgccggtg cgggagatcc tggcccgccg ggcccgccct
     1861 gcgggacgga ccgggccgga cgggtcgggc accgcggagg cttcgacggg ggtgcgggtg
     1921 gtcttcgccg ccggccgccc gattccggtc ccccggggct cggaattcct gctggtggag
     1981 accgggggac cggacgccgt ggacgcgcgg atcgaggtcc tggccgcgcc cggggacctg
     2041 gtggtgaccc gggacattcc cttcgcggcc cgcctggccg aggcggggct cgcggtgatc
     2101 aacgaccggg gcgacgagtg gacggcggac acggtccggg agcggctctc ccggagggac
     2161 ttcatggcgg gcctccgctc ctcgggcctt gcggagatga accgggcccg gacctggggt
     2221 ccccgggaga aaaaggcctt cgcggacacc ctggacaggg tggtacggag gatccaggcg
     2281 acaggacccc gaagtcagga ccccgggctg gcctccgatg tcggaggagg cgccggccgg
     2341 tccgcggccg acgggtccgt ccccggatcc tgagtcccga ccccgggctc ctctttctcc
     2401 ccgtacttcc ccgccaggta ctggtaccgc gtgcgcagga tcccgttctt gtgcagggtt
     2461 tccggggtgt agcggatggg ggacgagagc agggcgacca ggcgggcggt ctggtccacg
     2521 tccagacggg agacgctcgt tccgtagaac ctccgggccg cggcctcgat gccgaacact
     2581 cccttgcccc actccgccca ggaaaggtag atctccagga tcctctgctt gggcaggatc
     2641 agctccatct cgaccgtggc gatgagctcc aggtacttcc gcaggtagct cttgaagggt
     2701 accagaaaga gggtccgggc agtctgcatg gacagggtgg agccgccgta gaggggccgg
     2761 ccgacctgcc gattgacctg ccgggcatac aggatagcct cgaagtccac cccgttatgg
     2821 ctgtagaact tgccgtcctc cacggagacc accatgcgcc gggccaggac cggcaccttg
     2881 gccagcttga cgttccgctg gggcgccagc ttccagccgt ccaccccggc ccggtaggcc
     2941 atgagcacgg tgacgggggg gtcgacgctc cggtagacca ggaggaggag ggcgaccgtc
     3001 aggacgtacc agacatgggc cagggcgatc ccgcgccccg cggtccgaag gacccttccc
     3061 agggcggccc gggctttcag gatccgggct cggctcggcg acggcggggg ggataccggc
     3121 gggaccggca ccctcgcggg ctccggccct ccggcgtccg cccgagtccg gccccgtttc
     3181 ccggaggttc cggccgacct cggggtctcc ggacccgccc cgcgcgagac aagggcggcg
     3241 gcgcaggccc tgcccaggct tcgttccacg ggcgctccga agaggatgag ggctaggggg
     3301 atcgtatcca tgggtcgggg ggctctccgg atcgggcggt ttgccggccg gctcgggcgc
     3361 cgtcggcgat cgtcccgacg ataccacgaa ccccgtccct tggggaagcg cctccggctt
     3421 ccttgacagg agtggcgccg gctccttaga tttggatctt ggcgggctcg tggaacgctt
     3481 cgggcgttcc gatagcccgc cggactgccg gcaccgtccc ggcaggggag gttccgggaa
     3541 tgaaaaaagt ccgtttcgcg ctcttggtcg cgctggttct ttcatcgacc ttcctgtatg
     3601 cggaagccac cggggtgccg ggcctgcaca ggttcgtcct ggacaacggc ctggagctct
     3661 tcgtgctgga gaatccctcc gcgcctctgg cctacatcga gatcgcgatc cggggagggg
     3721 gcgtggccca gaccccggag accgcggggg tgttccattt ctatgagcac atgatgttca
     3781 agggcaactc gaagtacagg acggcggcgg aggtgagctc ggcctacctg gacctggggg
     3841 tgccctcctg gaacggctcc acgggttcgg aatacgtcga ctactatttc acggtgccct
     3901 ccgaccggct gcgggaaggg ctggagttct ggtcctacgc gatccgggaa cccctgctcg
     3961 acccggggga gatcgaatcc gagaagggcg tcgtcgtcag cgagatcacc ggaaactatt
     4021 ccgaaccccg cagcatctac gccgccggga tcgaccgcag gctcttcccg aaatacccct
     4081 ggcgccgaga tccgggagga accgtggagg ccatccgggg cgtgaccgcg gaaaagctcc
     4141 ggaccatgca gcggacctac tacatcccga acaacgccgc cctcttcgtg ggaggggacg
     4201 tgcgggcgga cgcggtccac cagttggtga aggaagtcta cggttcctgg gaacggggac
     4261 ccgatccctg ggccaagccc caggatcccc aggccttccc ggcggtccgc aagacggcct
     4321 tcgtggtcta cccggatccc acggtgaatc cccgtttcgg gatagcccag atctactacc
     4381 gggggcccga cgtgggccgg gatcccgtgg ccacctacgc cgcggacgtg tggggctacc
     4441 tggtggagaa cccctcggga cggt
//
LOCUS       DAOTEI010000233         4461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_14248_length_4461_cov_4.274626, whole genome shotgun sequence.
ACCESSION   DAOTEI010000233 DAOTEI010000000
VERSION     DAOTEI010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14248_length_4461_cov_4.274626"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..136)
                     /locus_tag="P5313_15705"
     tRNA            complement(64..136)
                     /locus_tag="P5313_15705"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:His,seq:gtg)
     gene            complement(220..1245)
                     /gene="amrS"
                     /locus_tag="P5313_15710"
     CDS             complement(220..1245)
                     /gene="amrS"
                     /locus_tag="P5313_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRY81861.1"
                     /translation="MIRRGRFWEAGEGGRVRCLLCHRSCSIPPGAAGICGVRRNEGGV
                     LMLPYYGRISSAAVDPIEKKPLLHFRPGGRVFSIGFTGCNLVCPFCQNWTISQDPDAP
                     SDYLSPADAVRAARDSGCGMIAYTYSEPLVHAEYVLDCMDRARAEGLANVLVTNGHAR
                     EEPAREILDRTDAANVDLKAWDPAWYRSELGGDLDTVKGFLGLAAERTRLEVTTLVIP
                     GRNDGDEDMRGIAGFLAGLSPDIPLHLSAYRPMYKYRLPPTPRDTLERLAAVARERLR
                     YVYLGNVPGAEDTRCPECGAVLVRREGYRTALENLVPSGPASSACGRCGASIPIVPPE
                     GRAGGGA"
     gene            complement(1247..1651)
                     /locus_tag="P5313_15715"
     CDS             complement(1247..1651)
                     /locus_tag="P5313_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81862.1"
                     /translation="MSIRDYLESEPFFEIQRYTSSPPQDAVAFTGTPRKHPYDDGKIV
                     LVGDPSAENPGVYEFLVGDIVHAEDLASPVTQSGESYPRVRIWIRRGSVGIRYEPFEV
                     DTPPRFFGISPDLKIARPSPGSRDRSAGGSRG"
     gene            complement(1629..3290)
                     /gene="lnt"
                     /locus_tag="P5313_15720"
     CDS             complement(1629..3290)
                     /gene="lnt"
                     /locus_tag="P5313_15720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRY81863.1"
                     /translation="MKRGSLILLALLGGALTALAVPNELFPRGNPALGWLAAAPLYFA
                     LAASPGYGFSAALGAVYGAAAHGFSSYWLWFFKDYAVWTLGSTVLAYAVVYAHFALWL
                     SFLLRRGGVCRPLAYALLWAGLEFYKSTGFLGYPWGLLAYSVNEVLPLIQIADRTGVY
                     GISFLLAWSGSSLAEIAGIPPVGLGSRARIVRSTFPTPLPEDGRDRRALGVGNLALVA
                     LTAVAVLSYGYARLARPVPEAGRIAAVLVQQNSDSWTDGEERTLLACMDLTRRAVAEG
                     GRRPDIVLWNESNLDAPYAEFFRKYSRYPGADPLVPFFREAGTRFLLGAPVAVDWETY
                     SFMNAVILVGPDASLEDQYGKIQLVPFAEGVPFWEYEWFRAFMDKVVGLGAGGWTPGT
                     ERVLFDLPTAGGRTFRFAAPICFEDAFSGLCRDFARDGADFFVNLTNDSWSRTVSALV
                     QHFAAARFRAVENRRTLVRSTNGGISCVVGAHGEVLAELPVFRAESRFLEIPVFREDR
                     DTVYTTYGDWFGYAALFLSGLFALILVLEERISTRRNRREHPRLP"
     gene            3518..3997
                     /gene="coaD"
                     /locus_tag="P5313_15725"
     CDS             3518..3997
                     /gene="coaD"
                     /locus_tag="P5313_15725"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRY81864.1"
                     /translation="MVKAVFPGSFDPPTYGHLNIIERARGIFEELYVVVAVNREKSYL
                     FSPDERLSLLKDLLKGYPNVSVHLCDTLIVDFARKYGCRVLLRGVRSVPDFSYEFELS
                     VMNKGLDPAIETFIMPTDPRYFVLRSSAIKELVAFNGDVSGMVPPSVAMALKGRISR"
     gene            4111..4296
                     /gene="rpmF"
                     /locus_tag="P5313_15730"
     CDS             4111..4296
                     /gene="rpmF"
                     /locus_tag="P5313_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRY81865.1"
                     /translation="MAVPRSKTSKARTSRRQSINMKLTAPAVVSCGNCGNPVLSHRVC
                     PKCGFYRGRQVLTPETK"
     gene            4309..>4461
                     /locus_tag="P5313_15735"
     CDS             4309..>4461
                     /locus_tag="P5313_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY81866.1"
                     /translation="MDELFAKIKKIIADKLEVEEDKITLEASFRQDLGADSLDTYELV
                     YGIEEEL"
BASE COUNT          820 a         1456 c         1497 g          688 t
ORIGIN      
        1 gctctatcca gctgagctac gggcgcatat cctaccgtcg tcgaccggcg gcgtggagcc
       61 cagggtgggt gacgggtctc gaacccgcga catccagatc cacattctgg tactctacca
      121 actgagctac acccaccgtg cgttgcactt ggtaggcagt acaatgccaa aacccgccgg
      181 gattgtcaag ggccgatccg tacttttccg gccgcctttt catgcccctc cgccggcccg
      241 gccctcgggc gggacgatgg ggatcgaggc gccgcacctg ccgcaggcgg aggaggcggg
      301 gcccgagggg accaggtttt ccagggccgt gcggtagccc tcccgccgga ccaggaccgc
      361 cccgcactcg ggacaccggg tgtcctccgc ccccgggacg ttccccaggt agacgtagcg
      421 gagacgctcc cgggctaccg ccgcgagccg ctccagggtg tcccgggggg tcggcggcag
      481 gcggtacttg tacatggggc ggtaggcgga caggtgcagg ggaatgtccg gggaaagtcc
      541 cgccaggaag cccgcgatcc cccgcatgtc ctcgtcgccg tcgttccggc ccgggatgac
      601 cagggtcgtg acctcgagcc gggtcctctc cgcggccagc ccgagaaagc ccttcaccgt
      661 gtccaggtcc ccccccagct cagagcggta ccatgcgggg tcccaggcct tgaggtccac
      721 gttcgcggcg tccgtgcgat ccaggatctc ccgggcgggc tcctcccggg cgtggccgtt
      781 ggtcaccagg acgttcgcca gtccctccgc ccgggcccgg tccatgcagt ccaggacgta
      841 ctccgcgtgg accaggggtt ccgagtaggt gtaggcgatc atcccgcagc ccgaatcccg
      901 ggcggcccgg acggcgtcgg cgggggagag gtagtccgaa ggcgcgtcgg gatcctggga
      961 aatggtccag ttctggcaaa aggggcatac caggttgcag cccgtgaagc cgatggaaaa
     1021 tacccgcccc cccgggcgga aatggaggag aggtttcttt tcgatgggat ccacggcggc
     1081 cgaggagatc cggccgtaat acgggagcat aaggacgccc ccctcgttgc ggcgtacccc
     1141 gcagattccc gccgcgcccg gggggatgga gcagctccgg tgacagagga gacagcggac
     1201 gcgcccgcct tccccggcct cccagaagcg gccccgccgg atcacctcag ccccgggacc
     1261 cgcccgcgct ccggtcccgg gagcccgggg acggccgggc gatcttcagg tccggggaga
     1321 tcccgaagaa ccggggaggg gtatccacct cgaagggctc gtaccggatc cccacgctcc
     1381 cccggcgtat ccagatccgg acccggggat agctctcccc gctctgggtg accggggagg
     1441 ccaggtcctc ggcgtggacg atgtccccga ccaggaactc gtagaccccg gggttctcgg
     1501 cggaggggtc ccccaccagg acgatcttgc cgtcgtcgta ggggtgcttc cggggggttc
     1561 ccgtgaaggc caccgcgtcc tggggcgggg aggatgtata cctctggatc tcgaagaagg
     1621 gctcgctttc aaggtagtcg cggatgctca cgccggttcc tcctggtcga gattcgttct
     1681 tccagcacaa gtataagggc gaagagcccg gaaaggaaga gcgcggcgta cccgaaccag
     1741 tccccgtagg ttgtatacac ggtatcccga tcctcccgga atacggggat ttccaggaac
     1801 cggctctcgg cccggaagac cggaagctcc gccagcacct ccccgtgggc gcctaccacg
     1861 caggagatgc ccccgttcgt ggaccgcacc agggtccggc ggttctccac ggcccggaac
     1921 cgggcggccg cgaagtgctg gaccagggct gaaaccgtcc gggaccagga atcgttggtc
     1981 aggttgacga agaagtccgc cccgtcccgg gcgaaatccc ggcagagccc cgagaaggcg
     2041 tcctcgaagc agatgggggc ggcgaagcgg aaggtccggc cgcccgcggt cggcaggtcg
     2101 aagaggaccc gctcggtgcc gggggtccag cctccggcac ccaggccgac gaccttgtcc
     2161 atgaaggccc ggaaccactc gtactcccag aagggcaccc cctccgcgaa ggggaccagc
     2221 tggatcttgc cgtactggtc ctccagggac gcgtccggcc ccaccaggat cacggcgttc
     2281 atgaaggaat aggtctccca gtccacggcc acgggggctc ccaggaggaa tctcgttccc
     2341 gcttcccgga agaagggcac cagggggtcc gcgccgggat accgggagta cttgcggaag
     2401 aattccgcgt acggggcgtc caggttggac tcgttccaca ggacgatgtc cggccggcgg
     2461 cccccctcgg cgacggccct gcgggtcagg tccatgcagg ccaggagggt ccgctcctcc
     2521 ccgtcggtcc aggagtccga gttctgctgg accaggaccg cggcgatacg ccccgcctcg
     2581 ggaaccgggc gcgccaggcg ggcgtatccg taggagagga ccgcgacggc cgtcagggcc
     2641 accagggcca ggttcccgac ccccagggcc cggcggtccc gtccgtcctc cggcaggggc
     2701 gttggaaacg ttgaccggac tatccgcgcc cgggagccga ggccgaccgg gggaatcccg
     2761 gcgatttccg ccagggagga cccggaccag gccaggagga aggagatacc gtacaccccg
     2821 gtccgatccg cgatctggat cagggggagg acctcgttca cgctgtaggc cagaaggccc
     2881 cagggatagc ccagaaaccc cgtggacttg tagaattcca gtcccgccca caggagggcg
     2941 taggccaggg ggcgacagac gcctccccgc cggagcagga aggagagcca gagggcgaag
     3001 tgggcgtaca cgacggcata ggccagaacc gtgcttccca gggtccagac ggcgtaatcc
     3061 ttgaagaacc agagccagta ggaggaaaac ccgtgggccg cggccccgta gacggcgccc
     3121 aaggccgcgg agaacccgta acccggggat gcggccaagg cgaaatagag aggcgcggcg
     3181 gccaaccaac ccagggcggg attcccccgg ggaaagagtt cgttcgggac cgcgagggcc
     3241 gtcagcgcgc cacccaggag ggccaggagg atcaggctgc cgcgtttcat cggaaggaat
     3301 aatacagggt gggagggaag tttgaaaggg ttaaagggtg aatgggtgaa taggtgaatg
     3361 ggtgaatggg tgaatgggtg aatgggtgaa tgggtgaatg ggtgaataag gaaggccgcc
     3421 agcgggttcg cagtccctcc tattcacgta ttcacgattc acatattcac ctcccgttac
     3481 cttgaccgcc cgctcccgat gctttagatt gccagctatg gtaaaggccg tgttcccggg
     3541 gtccttcgat ccccccacgt acggacacct taacatcatc gagcgggcac ggggcatctt
     3601 cgaggagctc tacgtggtcg tggccgtgaa ccgggaaaag agctacctgt tcagcccgga
     3661 cgagaggctc agtctgctca aggacctcct caagggctat ccgaacgtct ccgtacacct
     3721 ctgcgacacc ctgatcgtcg atttcgcccg gaagtacggc tgccgggtcc tcctgcgggg
     3781 ggtacgcagc gtcccggatt tttcctacga gttcgagctc tcggtcatga acaagggcct
     3841 ggaccccgcc atcgagacct tcatcatgcc cacggatcct cggtacttcg tcctgcggtc
     3901 cagcgcgatc aaggaattgg tggccttcaa cggagacgtc tccgggatgg tcccgccctc
     3961 ggtggccatg gccctgaagg gcaggatttc ccggtagcct ctgcaattcc ggccgccccc
     4021 ggccgctcca tcttgacaaa atccccggcc tccgttacgg ttgcgagtcg gtatccaatt
     4081 cggacaattc tacgggaatg aggtttagac atggctgtac ctagatcaaa gacttcgaag
     4141 gcccggacca gccgtcggca gtcgatcaat atgaaattga ccgctccggc cgtcgtgtcc
     4201 tgcgggaact gcggcaaccc cgtgctgtcg caccgcgtgt gcccgaagtg cggtttctat
     4261 cgcgggcgtc aggttctgac ccccgaaacc aagtaaagga gcattcacat ggacgaactt
     4321 ttcgccaaga tcaagaaaat catcgcggac aagctcgagg tcgaggagga caagatcacc
     4381 ctcgaggcca gcttccgcca ggacctcggc gccgacagcc tcgacaccta cgagctggtc
     4441 tacggcatcg aggaagagct c
//
LOCUS       DAOTEI010000234         4413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_14468_length_4413_cov_4.339605, whole genome shotgun sequence.
ACCESSION   DAOTEI010000234 DAOTEI010000000
VERSION     DAOTEI010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14468_length_4413_cov_4.339605"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="P5313_15740"
     CDS             <1..521
                     /locus_tag="P5313_15740"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRY81867.1"
                     /translation="EHSHYTRPEEVEDFVKKTGVDSLAISIGTSHGRAKFKPEQCTKD
                     ANGILIPPPLRFDILKEIEKRIPGFPIVLHGSSSVPVDYVKMIEKFGGKLSDSVGIPE
                     SQLREAAKSAVCKINIDSDGRLAMTALIRKVLAEKPDEFDPRKYLGPARDELKKLYAY
                     KNEHVLGSAGKA"
     gene            complement(729..1166)
                     /gene="rnhA"
                     /locus_tag="P5313_15745"
     CDS             complement(729..1166)
                     /gene="rnhA"
                     /locus_tag="P5313_15745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008229256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRY81868.1"
                     /translation="MADVIVYTDGACSGNPGPGGWAWVALQDGSRREGSGGEAATTNN
                     RMELIAVIEALKALPPGRAVEVRTDSQYVKNGITAWIHAWKKNGWRTASKSPVKNREL
                     WEDLDRLSTALKPRFVWVEGHAGEEFNERCDELARGEIARLRG"
     gene            complement(1171..3129)
                     /locus_tag="P5313_15750"
     CDS             complement(1171..3129)
                     /locus_tag="P5313_15750"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY81869.1"
                     /translation="MSSAGEVKPQGAAESTPVVAPVFRKAAGAVALVMTVAAVAVPVL
                     DFFILRIFQWGIPGATPFSIQAVLVLAFAGAVITSLEGEHLALTKTPHHGIDTRHRIQ
                     SSAVSFLTCAVEAAFVLGTLSFALLGFDPSERAGFVPYWIFALALPAGFLSMLVLDLR
                     RVKGWPRAAAAAGILAGVFLAAPSLVNLASLAGIFWPALDSLAGAAVASLRLLGLPLA
                     LVLGASAFLGTPLFVVLAGMAAILFTADGSLIELAVTESYAMLRSPQIAAVPLFTLTG
                     FLLAGTKAGERLVTLFRALFGRVRGGAAVAAVTVCAFFSVFTGSSGVAILALGPLLAR
                     VLVDAEGMGDETSKGLLTASGAVGLLFPPSMAVIVYAVTAQYSYGPENPLDILALFRS
                     SVIPGLLFVAVTAGAGIYFAAKERKGAAAAAPVPSAGTPAAPDMRPSIRRSLWAALPE
                     SFLPFGIALLFFSGTATLMEIGALSILYTGAYGFLRKEFDLRSLAASAAKAFPVIGGT
                     LILLAAARAFSYFLVDSGIPDMAAAFVTSQISSKLLFLLLLNLLLLAAGCLMDVFSAI
                     LVLAPLVIPVGQAFGIHPIHLGIIFVSNLMVGFITPPIGMNLFLASYAFDRPLGRVWR
                     AVSPWFLLQLAVVILITYVPALSLVFAG"
     gene            complement(3126..4118)
                     /gene="dctP"
                     /locus_tag="P5313_15755"
     CDS             complement(3126..4118)
                     /gene="dctP"
                     /locus_tag="P5313_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY81870.1"
                     /translation="MNRRTPILFLMLVLAAASLFGQVNLKLGVAAPKNSPWGMSLDRL
                     AADWRRISGGKVTLTVYAGTLGDDNSIIQKMRFGLDAGVFPSTGLSLIYEDILALSIP
                     SLVRDDAELRAVLKELDGPFRAEIGRKGYTILTYSSAGWVWLFSRRPVAKPSDIQNLK
                     VAVPVDDRKMSRMLQAAGAVPVQVDIQNLVGQIATGAVDALLISPAFVQAQWSFFKSY
                     LPYVSDMKISPFVGAALINTRSWERVPADLRKPLMDAADKMAGDLAREADRLDDVAIK
                     AMTSDGLSVVPLTAADRAAWYEVFVANRDRFVRDLFSKDVLEAIDRVVRSRSRP"
     gene            complement(4140..>4413)
                     /locus_tag="P5313_15760"
     CDS             complement(4140..>4413)
                     /locus_tag="P5313_15760"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HRY81871.1"
                     /translation="RELGGGEERAEAAYRRALEASGGLRASPYVSYASSVCVARQDYK
                     AFKAALEAALAVDVDADPSGRLANIIAQRSAKRLLAQAPDIFLILE"
BASE COUNT          791 a         1437 c         1567 g          618 t
ORIGIN      
        1 cggagcacag ccactacacc cgacccgagg aggtggagga cttcgtgaag aagaccggcg
       61 tcgactccct ggccatctcc atcgggacca gccacggccg ggccaagttc aagcccgagc
      121 agtgcaccaa ggacgccaac ggcatcctga tcccgccccc cctgcgcttc gacatcctca
      181 aggagatcga gaaacgcatc cccggcttcc ccatcgtcct gcacgggtcc tccagcgtac
      241 ccgtggacta cgtgaagatg atcgagaagt tcggcggcaa actatccgac tccgtgggca
      301 tccccgagtc ccagctccgg gaggccgcca agagcgcggt gtgcaagatc aacatcgact
      361 ccgacggacg cctggccatg acggccctaa tccgcaaggt cctggcggag aagcccgacg
      421 agttcgaccc ccgcaagtac ctgggtcccg cccgggacga gctcaagaag ctctacgcct
      481 acaagaacga gcacgtcctg gggagcgctg ggaaggctta gcgcgagacg ggcgaagtcc
      541 gtctcgcggc aacgaagccc tccgggcttc gtcgaagtac aggaaagggg gtcgggtata
      601 accggtcccc tttcctggaa atctgttcaa tcctgttctg aatcgccagg ttctgctatt
      661 tttttcttct ttcttctctc ttctccgtgc tctccgtgtc ctccgtggtg attccgggat
      721 ttatcctctc atccccgcag ccgcgctatc tccccccggg ccagttcgtc gcagcgctcg
      781 ttgaactcct ccccggcgtg gccctcgacc cagacgaacc tgggctttaa ggcggtcgaa
      841 aggcggtcca ggtcctccca gagttcccgg ttcttcacgg gggatttcga ggcggtcctc
      901 catccgttct tcttccaggc atggatccag gcggtgatcc cgttcttgac gtactgggag
      961 tccgtccgga cctccacggc ccggcccggg ggcagggctt tcagggcctc gatgaccgcg
     1021 atcagctcca tgcggttgtt cgtggtggcg gcctcgcccc cggatccttc ccggcggctg
     1081 ccgtcttgga gggccaccca ggcccagccc ccggggccgg ggttcccgga gcaggccccg
     1141 tcggtgtaca cgatcacgtc cgccacggcg tcagcccgcg aagaccaggc tcagggccgg
     1201 gacgtaggtg atcaggatga ccaccgccag ctggagcagg aaccaggggg acacggcccg
     1261 ccagaccctg cccaagggac ggtcgaaggc gtaggaggcc aggaagaggt tcatcccgat
     1321 gggaggggtg atgaagccca ccatcaagtt cgacacgaag atgatcccca ggtggatggg
     1381 gtggatcccg aaggcctggc cgaccgggat gaccaggggc gccaggacca ggatggcgga
     1441 aaaaacgtcc atcaggcagc ccgcggcaag gagaaggagg ttcagcagga gcaggaagag
     1501 gagcttggag ctgatctggg aggtcacgaa ggccgcggcc atgtccggga tccccgagtc
     1561 caccaggaag tacgagaagg cccgtgcggc ggccaggagg atcagggtgc ccccgatgac
     1621 ggggaaggcc ttggctgcgg aggcggccag ggaccggagg tcgaactcct tacgcaggaa
     1681 gccgtaggca ccggtgtaca ggatcgagag ggcgccgatc tccatgaggg tggcggttcc
     1741 cgagaagaac aggagggcga tcccgaaggg caggaaggat tccgggaggg ccgcccagag
     1801 ggatctccgg atcgagggcc gcatatcggg cgcggccggg gtacccgccg agggtacggg
     1861 cgcggcggcc gcggccccct tccgttcctt ggcggcgaag tagatcccgg cccccgccgt
     1921 gacggccacg aaaagcaggc ccgggatcac gctggaccgg aagagggcca ggatgtccag
     1981 ggggttctcg gggccgtagg aatactgggc cgtaaccgcg tacacgatca ccgccatgct
     2041 cggcgggaac aggaggccca ccgccccgct ggcggtcagg aggcccttgg aggtctcgtc
     2101 ccccatgccc tcggcgtcca ccaggacccg ggccaggagg ggccccaggg ccaggatggc
     2161 caccccggag gaaccggtga acaccgagaa gaaggcgcag accgtgacgg ccgccaccgc
     2221 cgccccgccc cggacccggc cgaagagggc ccggaacagg gtcaccaggc gctcccctgc
     2281 cttggtaccc gccaggagga atcccgtcag ggtgaacagg gggaccgcgg cgatctgggg
     2341 gctccggagc atggcgtagg attccgtgac cgcgagctcg atgagggaac cgtccgcggt
     2401 gaacaggatc gccgccatcc cggccaggac cacgaagagg ggggttccca gaaaggcgga
     2461 agctcccagg actagggcca ggggcagtcc gaggagccgc agcgaggcta cggcggcccc
     2521 ggccagggaa tccagggccg gccagaagat tccggccagg gaggccaggt tcacgaggct
     2581 gggggcggcc aggaagaccc ccgcgaggat gccggccgcg gccgccgccc ggggccatcc
     2641 cttcaccctg cgcaggtcca ggaccagcat ggacaaaaaa cccgcgggca gggccagggc
     2701 gaagatccag taggggacga agcccgcccg ctcggagggg tcgaagccca ggagggcgaa
     2761 ggagagggtc cccaggacga aggcggcctc cacggcgcag gtcaggaagg agacggcgga
     2821 ggactgtatc ctgtgccggg tgtcgatgcc gtggtggggg gtcttggtca gggccaggtg
     2881 ttcgccctcc agggaggtga tcacggcgcc cgcgaaggcc aggaccagga cggcctggat
     2941 ggaaaagggc gtggcccccg ggatccccca ctggaagatc cggagtatga agaagtccag
     3001 gaccggaacc gccaccgcgg cgacggtcat gaccagggcc acggcgcccg cggccttgcg
     3061 gaacaccggg gcgaccacgg gcgtcgattc cgcggcgccc tggggtttga cctccccggc
     3121 cgaactcacg ggcggctccg ggagcgcacg acccggtcga tggcctccag gacgtccttg
     3181 gagaagagat cccggacgaa gcggtcccgg ttggccacga agacctcgta ccaggcggcc
     3241 cggtccgcgg ccgtcagggg tacgacggat aggccgtcgg aggtcatggc cttgatggcc
     3301 acgtcgtcca ggcggtccgc ctcccgggcc aggtccccgg ccatcttgtc cgccgcgtcc
     3361 atgaggggct tgcggaggtc cgcgggaacc cgttcccagc tccgtgtgtt gatgagggcc
     3421 gcccccacga agggtgagat cttcatgtcg ctgacgtagg gcaggtagga cttgaagaag
     3481 gaccactggg cctggacgaa ggccggggag atgaggaggg cgtccacggc cccggtggcg
     3541 atctggccca ccaggttctg gatgtccacc tggaccggga cggccccggc ggcctggagc
     3601 atccggctca tcttgcggtc gtccacgggc accgcgacct tgaggttctg gatgtccgag
     3661 ggcttggcta ccggccggcg ggagaagagc cagacccagc ccgcggagga gtaggtcagg
     3721 atcgtgtagc ccttgcggcc gatctcggcg cggaaggggc cgtccagctc cttgagcacc
     3781 gcccggagct ccgcgtcatc ccggaccagg ctggggatgc tcagggccag gatgtcctcg
     3841 tagatcaggg acagacccgt gctggggaag accccggcgt ccaggccgaa gcgcatcttc
     3901 tggatgatgg agttgtcgtc ccccagggtg cccgcgtaga ccgtcagggt caccttgccg
     3961 ccggagatgc gccgccagtc cgcggccagc cggtccaggc tcatgcccca ggggctgttc
     4021 ttgggggctg ctacgcccag cttcaggttt acctggccga acagggaggc ggcggccaat
     4081 acgagcatga ggaacaggat cggggttctg cggttcacgg ttagggacct ccgttcggat
     4141 tattcgagta tcaggaaaat gtccggcgcc tgggcaagga gccgcttggc gctccgctgg
     4201 gcgatgatgt tggccagcct gcccgaggga tccgcgtcga catccaccgc cagggcggcc
     4261 tccaaagccg ccttgaaggc cttgtagtcc tggcgggcga cgcacaccga ggacgcgtag
     4321 gagacgtaag gggaggcccg gagccccccg gaagcctcca gggcgcgccg gtaggccgcc
     4381 tcggcccgct cctcgccgcc gcccagctcc cgg
//
LOCUS       DAOTEI010000235         4262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_15197_length_4262_cov_5.096030, whole genome shotgun sequence.
ACCESSION   DAOTEI010000235 DAOTEI010000000
VERSION     DAOTEI010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15197_length_4262_cov_5.096030"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="P5313_15765"
     CDS             complement(<1..1376)
                     /locus_tag="P5313_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY81872.1"
                     /translation="MRRNRCIPRSILAAAAFLSLQAPAALAQEAPDKELVIAVTSLDL
                     EIHPHHSIYAQEAQILSGLYEGLFSYNPATLEPVRAAIRSFSRSRDGKTYTFYLRETA
                     RWSNGDRVTAAHFRDAWLRMMDPAVKADYGSFFDIIAGARDYRAGRSRDTSKVGVSAI
                     SEDVLQVTLESPAPWFTKLLCHHSFSPVHPSVLGVRDWLSRAASVPYNGAFRIESVGP
                     EGFTLRKNEAYWDADSVRLPGIRVVAPESPEEASRLFNNGAVHWLAGDVDLESILYRD
                     AIQVNPMFATHYWFFSCRSAPWSDPAVRRALALLAPWDEIRSREQYLIPATGLVLPVE
                     GYEAPAGIREPKPHAARALLESAGYPDGKGLPALRILLPEGEGAVRVAGLLSKAWKEA
                     LGLEMVVKTLPASRFYGSLRGADWDLTLNTWIGDFADPLAFLQMWTSDSSLNDAGYSD
                     PDYDRRIA"
     gene            complement(1399..2823)
                     /locus_tag="P5313_15770"
     CDS             complement(1399..2823)
                     /locus_tag="P5313_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81873.1"
                     /translation="MEGNGEYERILEEKLLAKAETLDRVELPKLKEAFKLFQTAFQGI
                     HTVLLRKGVIQKDPYKFDLKISEVQTPPESPFAESEKNEQISVRLSQFDSYLDFLNGY
                     YQFSVDFLTMGRVKRLINFTKYFNFLQFSETSNQLNTRCFAEVVGLVRKGTDPLSTGI
                     LGEGLLSLEKTSREIFQILKSLTLYHKERYKLDIRQLALAGLTLDRERVITHRDEALR
                     SIKRKFSETMGDRPFYPELVEEVLAEDYSSDGPNLRDDILAQLQVKEEKKADSAQARN
                     FKAIVLDGARVLAGAGYQLEEAIRKLRENSILLENQNRTFLGQMRKMIRRLFNPAEDN
                     VVYQVEYSDPITAEHRREEIDFTRLSEEIARRARVLASMMARNSQSWQRLASAPEEQA
                     YSFLVKGLEDLQRYYRRLSALDEHFKNEITDPEAKPRIRGIKVELATLKTSIIKANQK
                     KHEYVAQKEEIEQMRRLGIRAETP"
     gene            2845..3399
                     /locus_tag="P5313_15775"
     CDS             2845..3399
                     /locus_tag="P5313_15775"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HRY81874.1"
                     /translation="MPLKASMGETSEFEPALLFLGLLYPASEPGPAEEAWSRLSARWG
                     AEGWRSPDIPFTWSPYYDEELGSPILRSLRCFREPVDPSGLAEAKVFTNRVEAELSRD
                     GRRRVNLDPGLLFPSRLILATTKDRAHRIPLRDGIFAELTLLYEKGGWKPLPWTYPDY
                     RTPEYAEALTRMREILRAGRKRKA"
     gene            3489..4214
                     /locus_tag="P5313_15780"
     CDS             3489..4214
                     /locus_tag="P5313_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81875.1"
                     /translation="MPALRLLAAVPVLVAFGAACRLFPEWEDVTVRQPETPAAWAGCG
                     LSPDWEVRAFWFGGRAVSAAGSGGRASLLLPRGERVAVLAYPIRAGRELRPAGALYPS
                     DGEGTLALTWEGGYRAEAARVLILAGVDPGRFDLDRFAREALTRLGDPWLRPPAAFAE
                     SLAAETFRVTWLDPSPLFAASVGGLPGSAASESPFGSSLIPDGEGQAAVSLPPGVHRW
                     YADWGRVTVELREDGEAARVIIR"
BASE COUNT          645 a         1371 c         1498 g          748 t
ORIGIN      
        1 tcggcgatcc gtcggtcgta gtccggatcc gagtagcccg cgtcgttcag ggatgaatcg
       61 gaggtccaca tctgcaggaa ggccaggggg tccgcgaagt cgccgatcca ggtgttcagg
      121 gtcaggtccc aatcggcgcc ccggaggctg ccgtagaacc gggaggcggg gagggtcttg
      181 acgaccatct ccagccccaa ggcttccttc caggccttcg acaggagccc ggccacccgg
      241 acggcccctt ccccttcggg aagcaggatc cgcagggcgg gaagcccctt gccgtccggg
      301 taccccgccg attccaggag ggcccgggcg gcgtggggtt tcggttcgcg gatgcccgcg
      361 ggcgcctcgt agccctccac gggcaggacc aggcccgtcg cgggaatcag gtactgctcc
      421 cggctgcgga tctcgtccca gggggccagg agggccaggg cgcggcggac cgcgggatcc
      481 gaccaggggg cggaccggca ggagaagaac cagtagtggg tggcgaacat gggattcacc
      541 tggatcgcgt cccggtacag gatgctctcc aggtccacgt ccccggccag ccagtggacg
      601 gccccgttgt tgaagagccg ggaggcctcc tcgggggatt ccggggctac aacccggatc
      661 ccgggaagcc ggacggaatc ggcgtcccaa taggcctcgt tcttccggag cgtgaagccc
      721 tcggggccga cggactcgat gcggaaggct ccgttgtagg gcaccgaggc ggcccgggac
      781 agccagtccc ggacgcccag gacggagggg tgcacggggg agaaggagtg gtggcagagg
      841 agcttcgtga accagggagc cggggattcc agagtcacct ggaggacgtc ctcggaaatc
      901 gcggataccc cgaccttcga ggtgtcccgg gaccgccccg cacggtagtc ccgggctccc
      961 gcgatgatgt cgaagaagga tccgtagtcc gccttgaccg ccggatccat catccggagc
     1021 caggcgtccc ggaagtgggc cgcggtcacc cgatccccgt tggaccatcg ggccgtctcc
     1081 cgcaggtaga acgtgtaggt cttcccgtcc cgggaacggc tgaagctgcg gatcgcggca
     1141 cggacgggct ccagggtggc cgggttgtag ctgaacagtc cttcgtagag ccccgagagg
     1201 atctgggcct cctgggcgta gatggagtgg tggggatgga tctccaggtc cagggaagtg
     1261 acggcgatca ccagttcctt gtccggggcc tcctgggcga gggccgcggg agcctgcagg
     1321 gacaggaagg ccgcggccgc caggatcgac cgggggatac agcggttccg acgcatacgg
     1381 gggactcctt tcgagccgct acggagtttc cgcgcggatg cccagacggc gcatctgctc
     1441 gatctcctcc ttctgggcga cgtactcgtg cttcttctgg ttggccttga tgatggaggt
     1501 cttgagggtg gccagctcca ccttgatccc ccggatcctg ggcttggctt ccggatccgt
     1561 gatctcgttc ttgaagtgct cgtccagggc ggaaaggcgg cgataatagc gctggaggtc
     1621 ctccaggccc ttcaccagga agctgtaggc ctgctcctcc ggcgcggagg ccaggcgctg
     1681 ccaggactgg gagttgcggg ccatcatgga cgccaggacc cgcgcccggc gggcgatctc
     1741 ctcggagagc cgggtgaagt cgatctcctc cctccggtgc tccgcggtga tggggtcgga
     1801 gtattcgacc tggtacacca cgttgtcctc cgcgggattg aagaggcggc ggatcatctt
     1861 gcgcatctgc cccaggaagg tacggttctg gttctccagg aggatcgagt tctcccggag
     1921 cttccggatg gcctcctcca gctggtagcc tgcccccgcc aggacccggg ccccgtccag
     1981 gacgatggcc ttgaagttcc gggcctgggc cgaatccgcc ttcttctcct ccttgacctg
     2041 gagctgggcc aggatgtcgt cccggaggtt cgggccgtcg gaggagtaat cctccgccag
     2101 gacttcttcc accagctcag ggtagaaggg ccggtctccc atggtctcgg agaatttccg
     2161 cttgatgctc cggagagcct cgtcccggtg ggttatcacg cgctcgcggt ccagggtgag
     2221 cccggccagg gccagctggc ggatgtcgag cttgtagcgc tccttgtggt acaaggtcag
     2281 gcttttcagg atctggaaga tctcccggga ggtcttttcc aggcttaaaa gcccttcccc
     2341 caggatcccc gtggacaggg gatccgtgcc cttgcggacc aggcccacga cctcggcgaa
     2401 gcaccgggtg ttgagctggt tgctggtctc ggagaactgc aggaagttga agtacttggt
     2461 gaagttgatc agccgcttca cccggcccat ggtgaggaag tccacgctga actggtagta
     2521 gccgttgaga aaatccaggt aggaatcgaa ctgggacagg cggacgctga tctgctcgtt
     2581 cttttccgac tccgcgaagg ggctctccgg gggggtctgg acctcggata tcttgaggtc
     2641 gaacttgtag ggatccttct ggatgacgcc cttgcggagc aggaccgtgt gtatcccctg
     2701 gaaggcggtc tggaagagct tgaaggcctc cttgagcttc ggcagctcca cccggtccag
     2761 ggtttcggcc ttggccagaa gcttctcctc caggatgcgt tcgtactcgc cgttcccttc
     2821 catagctcac cagtatggtc taaaatgccc ttgaaggcaa gcatgggaga gacttcggag
     2881 ttcgaaccgg ccctgttgtt cctggggctc ctgtatcccg cctcggaacc cggacccgcg
     2941 gaggaagcct ggagccggct gtccgcccga tggggggccg aggggtggcg gagccccgac
     3001 atccccttca cctggtcccc gtactacgac gaggagctgg gctccccgat cctccggtcc
     3061 ctgcgctgct tccgggagcc ggtggacccc tccggcctgg cggaggcgaa ggtcttcacg
     3121 aaccgcgtgg aggccgagct ctcccgggac gggcgccgtc gggtgaacct ggacccgggc
     3181 ctgctctttc ccagccgtct catcctggcc accaccaagg accgggccca ccggatcccc
     3241 ctgcgggacg ggatcttcgc ggagctgacc ctcctgtacg agaagggcgg gtggaagccc
     3301 ctgccctgga cctacccgga ctaccgcacc ccggaatacg ccgaagccct gacccggatg
     3361 cgggagatcc tacgggccgg acgcaagcga aaagcctagt ccgaggcgga ttcccgttca
     3421 tgctgccctc gatcccccga tccctcggag cggggtgccc cggattcgga ccggggctcc
     3481 gccgaggttt gcccgcgctt cgtctcctgg ccgctgtccc cgtcctcgtg gccttcgggg
     3541 cggcctgccg cctcttcccc gaatgggaag acgtcaccgt acgccagccg gaaacgcccg
     3601 cggcctgggc cgggtgcggc ttgtctcccg actgggaggt ccgggccttc tggttcggcg
     3661 gccgggccgt atccgctgcc gggtccggcg gccgggcctc actcctcctg ccccgtggcg
     3721 agcgggttgc ggtcctggcc tacccgatac gggccggccg tgagctccgg cccgcgggag
     3781 ccctgtatcc ctcggacggg gaggggaccc tcgccctgac ctgggagggc ggttaccgcg
     3841 ccgaggccgc ccgggtcctg atcctcgcgg gggtcgatcc ggggcgcttc gacctggatc
     3901 gcttcgcccg ggaggctctt acgcggctcg gggatccctg gctccggcct ccggcggcct
     3961 tcgcggagag cctcgccgcg gagaccttcc gtgtgacttg gttggacccg tctcccctgt
     4021 tcgcggcatc ggtcggcggc ctgcctggat ccgcggcctc cgaaagcccc ttcggctcaa
     4081 gtttaatacc ggacggggag gggcaggccg cagtgtcgct gcctccgggg gtgcatcggt
     4141 ggtacgcgga ctggggccgg gtgacggtgg agctgcggga agacggagag gcggcgaggg
     4201 tgataataag gtagcagccg acaggagtac ggcgacagat gaatacagag tcgacggatg
     4261 at
//
LOCUS       DAOTEI010000236         4254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_15257_length_4254_cov_3.622291, whole genome shotgun sequence.
ACCESSION   DAOTEI010000236 DAOTEI010000000
VERSION     DAOTEI010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15257_length_4254_cov_3.622291"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="P5313_15785"
     CDS             <1..277
                     /locus_tag="P5313_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY81876.1"
                     /translation="AGLARRGAQPYYLFQADLAPGTAHLRCPLSRGLDIYAELRRRLS
                     GLELPRYAVDAPGGGGKVYLPEGIIGREGDRYILETPFGGRAAYPAD"
     gene            399..929
                     /locus_tag="P5313_15790"
     CDS             399..929
                     /locus_tag="P5313_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81877.1"
                     /translation="MKPVPAAVAAVLVLALMSACVHPGTKAPPSGDPVSGQRNPPPAA
                     PAKPESPLSALPPETRVFLVELRDRVRRGDWAWAADRADPSFLRAMEGRGRDPYFYTY
                     LFAAGSLAGDAPEYARFELLPVSRVRDMAWEEARIEGPAAVVRGRFILSTGALVPFAL
                     RVLWRLDPPRILGVQP"
     gene            complement(1053..2603)
                     /gene="guaA"
                     /locus_tag="P5313_15795"
     CDS             complement(1053..2603)
                     /gene="guaA"
                     /locus_tag="P5313_15795"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRY81878.1"
                     /translation="MDSILILDYGSQYTQLIARRIREIGIYASILPGDARIGAPDLEG
                     VKGIILSGSPWSVYEPGAPAPDLVVYESGLPVLGVCYGIQRMTADFGGSVRTLPDREY
                     GRQAVRVEEDNALTRGLPRSFLSWMSHGDSVEIPAPGFRVLARSETGIPALLAHESRP
                     LWGVQFHPEVTHCEHGLEILGNFAAGICGAAREWSMERYVEEAGGRIRDAVGSGDVLL
                     LISGGVDSTVAAALVLKSLDPGRVHLMYIDTGLMRKGESEQVMRTLRSLGATHVHRID
                     AEGDFLTVLSGIDEPEEKRKRIGDMFITVQEREVARLGIPEAFLVQGTLYTDLIESGK
                     GVGKKAHVIKSHHNVRSPLVEKKRQAGKVIEPLDRLYKDEVRALGRLLGVPEEVVGRH
                     PFPGPGLGVRVLGEVTKEKCDVLREADDIYVSELKRRGLYDEIWQAFAVLLPVRSVGV
                     AGDVRRYGWVLALRAIVSSDGMTADVYPFPMKDLLEISSLITNRVPQIGRVTYDVSSK
                     PPATIEWE"
     gene            complement(2588..3817)
                     /gene="purA"
                     /locus_tag="P5313_15800"
     CDS             complement(2588..3817)
                     /gene="purA"
                     /locus_tag="P5313_15800"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRY81879.1"
                     /translation="MNIVVIGAQWGDEGKGKIVDYLAADAGLIVRFSGGANAGHTIVL
                     GKEQYALHLVPSGILYPDKITVLGSGMVIDPEALFAELAMLESRGVDWKGRVLISDRA
                     HIVLPRYREMDKQQEATRRKPIGTTGRGIGEAYSVKAARDGIRLADLEDAERLATLED
                     EDRAFLARYAERLLPMSVDLVAFLRQHRNSWTLFEGAQGALLDIDTGTYPYVSSGMSN
                     ASGAAAQGGIGPRAIDKVLGVFKAYTTRVGNGPFPTEFDPDSQGALCSFIRDTGREYG
                     VTTGRPRRCGYLDLVALRYARYANSIDSLVLTHLDVYDTLDEFEACVAYKIRGRVVED
                     FPTSVRDLNDARPVLRKFRGWKTSLGAARTWDDIPREAREYVAFIEEFAEAPVGIVSV
                     GYERNQTLVRESPWTRS"
     gene            complement(3830..>4254)
                     /locus_tag="P5313_15805"
     CDS             complement(3830..>4254)
                     /locus_tag="P5313_15805"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRY81880.1"
                     /translation="GDVVKSVAAGASSVMIGNLFAGLKESPGREILYEGRIYKEYRGM
                     GSLGALKDGAGDRYQMAKDEEPVPEGIEGRIPYKGELKPYLGQIVAGLRKGMGYTGCR
                     SIQELQKYGRFVRITSAGLRESHAHDVAITQEAPNYSR"
BASE COUNT          647 a         1491 c         1421 g          695 t
ORIGIN      
        1 tgcggggctg gctcggaggg gggcccagcc ctactacctc ttccaggcgg atctcgctcc
       61 cggcacggcc cacctccgtt gtcccctctc ccgggggctc gacatctacg cggagctccg
      121 gcgacggctt tcggggctgg agcttccccg gtatgccgtg gacgcacccg ggggcggcgg
      181 caaggtctac ctgcccgagg gaatcatcgg ccgggaggga gaccggtaca tcctggagac
      241 gcctttcggc ggccgcgccg cctatcccgc ggattgaaac cggccgccgc cccggaccgc
      301 ggacgcgagg gatcccggct cgatgtccgg gcgttgcttt cccccggggg atggctagaa
      361 tgaaggcttg cggatgttcc tgtagggagg agcacgtgat gaaacccgtt ccggccgccg
      421 tcgcggccgt cctggtcctg gccctgatgt ccgcctgcgt ccaccccggg accaaggccc
      481 ctccctccgg ggatcccgtt tcgggacagc ggaatccacc gcccgccgcc cccgcaaagc
      541 cggaaagccc cttgtcggcc ctgcctccgg aaacccgggt gttccttgtc gaactccggg
      601 accgtgtccg gcgcggggac tgggcctggg ccgcggaccg ggcggacccg tccttcctgc
      661 gggccatgga aggccggggc agggatccct acttctacac ctacctcttc gccgcaggct
      721 ccctggcggg ggacgcaccg gagtacgccc gcttcgaact gcttcccgtc tcccgggtcc
      781 gggacatggc ctgggaagag gctcggatcg agggacccgc ggcggtggtc cggggccggt
      841 tcatcctgtc gacgggagca ctcgtgccct tcgccttgcg ggtgctctgg cgtctggacc
      901 cgccccgcat cctgggcgtg caaccgtgaa agcgactact taaacaaacc aaccacagag
      961 gcacagaggc actgagaaaa gagaagtgcg gatgactcga cgaagcccgc agggcttcgt
     1021 tgccgcgagg cgggccttag gcccgcctcg cgctactccc attctatcgt ggccgggggc
     1081 ttggaggaga cgtcgtaggt gacacggccg atctggggca cccggttcgt gatcaggctg
     1141 gagatctcca ggaggtcctt catggggaag ggatacacgt ccgcggtcat gccgtccgaa
     1201 gagacgatgg cccgcagagc caggacccag ccgtagcggc ggacgtcccc ggcaacgccc
     1261 acggagcgga cgggcagcag gaccgcgaag gcctgccaga tctcgtcgta cagtccccgg
     1321 cgtttcagtt cggagacgta gatgtcatcg gcttcccgca ggacgtcgca cttctccttc
     1381 gtcacctccc ccaggacacg gacgcccagg ccggggcccg ggaagggatg ccggcccacg
     1441 acctcctccg ggacacccag caggcggccc agggcgcgga cctcgtcctt gtagagcctg
     1501 tccaaaggtt cgatcacctt tcccgcctga cgctttttct ccaccagggg ggagcgtacg
     1561 ttgtggtggc tcttgatgac gtgggccttc ttgcccaccc ccttgccgga ctcgatgagg
     1621 tccgtgtaca gggttccctg gaccaggaag gcctcgggga tgcccaggcg cgcgacctcc
     1681 cgctcctgga cggtgatgaa catgtccccg atccgcttgc gcttctcctc gggctcgtcg
     1741 attcccgaca ggacggtgag gaagtcccct tccgcgtcga tcctgtggac atgggtcgcc
     1801 ccgaggctcc ggagagtccg catgacctgt tcgctctctc cctttcgcat gagccccgtg
     1861 tcgatgtaca tgagatggac ccgtcccggg tcgagggact tgagcaccag ggccgctgcg
     1921 accgtggaat ccacgccgcc ggagatgagg agcagtacat ccccggagcc cacggcgtcc
     1981 cgtatccgcc cgcccgcctc ctccacgtaa cgctccatgc tccattcccg ggcggctccg
     2041 cagatcccgg cggcgaaatt gccgaggatc tcgaggccgt gctcacagtg ggtgacctcg
     2101 ggatggaact gcacgcccca gaggggccgg gactcgtggg ccaggagggc cggaatgccc
     2161 gtctccgagc gggccaggac ccggaaaccc ggggccggga tctccacgga gtcgccgtgg
     2221 ctcatccagg acaggaagct ccggggaagc ccccgggtca gggcgttgtc ctcctcgacc
     2281 cggaccgcct ggcggccgta ctcccggtcc ggcagggtcc gcacggagcc gccgaagtcc
     2341 gcggtcatcc gctggatgcc gtagcagact cccaggacgg gaaggccgga ctcgtacacg
     2401 accagatccg gggccggggc gcccggctcg tacacgctcc agggggatcc ggaaaggatg
     2461 attcccttca cgccctccag gtccggtgcg ccgatcctag cgtccccggg aaggatggaa
     2521 gcgtagatcc cgatctcccg gatccgtcgg gcgatcaact gggtgtactg gctcccgtag
     2581 tccaggatca ggatcgagtc cacgggctct ccctgacgag ggtctggttg cgctcgtagc
     2641 ccacggagac gatccccacg ggggcttccg cgaactcctc gatgaaggcg acgtactccc
     2701 gggcctcccg ggggatgtcg tcccaggtcc gggccgctcc cagggaggtc ttccagcccc
     2761 ggaacttgcg caggacgggc cgggcgtcgt tgaggtcccg gacggaggtg gggaagtcct
     2821 ccaccacccg gccccggatc ttgtaggcca cgcaggcctc gaactcgtcc agggtgtcgt
     2881 agacgtccag gtgggtgagc accagggaat cgatggagtt ggcgtaccgg gcgtagcgca
     2941 gggccaccag gtccaggtag ccgcaacgcc tcggccgtcc cgtggtcact ccgtattccc
     3001 gtccggtgtc ccggatgaag ctgcacaggg cgccctggga atcgggatcg aactccgtgg
     3061 ggaagggacc gttccccacc cgggtcgtgt aggccttgaa gacccccagg accttgtcga
     3121 tggcccgggg gccgatgccg ccctgggccg ccgccccgga ggcgttggac atcccgctgg
     3181 aaacgtaggg gtaggtcccg gtatcgatgt ccaggagggc cccctgggct ccctcgaaga
     3241 gggtccagga gttacggtgc tgccggagga aggcgaccag gtccacggac atcggcagca
     3301 ggcgttccgc gtaccgcgcc aggaaggccc gatcctcatc ctccagggtg gccaaacgtt
     3361 ccgcatcctc caggtccgca agacggatcc cgtcgcgggc ggccttgacg gagtacgcct
     3421 ccccgatccc ccggccggtg gtgccgatgg gcttgcgtcg ggtcgcctcc tgctgcttgt
     3481 ccatctcccg gtaccggggc aggacgatgt gggcccggtc gctgatcagg acccggccct
     3541 tccagtccac gccccgggac tccagcatgg ccagctccgc gaagagggcc tccgggtcga
     3601 tgaccatacc gctgcccagg accgtgatct tgtccgggta gaggatgccc gagggaacca
     3661 agtgcagggc gtactgctcc ttgcccagga cgatggtatg cccggcgttg gcccccccgg
     3721 agaagcggac gatgagcccc gcgtccgcgg ccaggtagtc cacgatcttt cccttgccct
     3781 cgtcgcccca ctgggcaccg atcacgacga tgttcacgtc agcctcccgt cacctgctgt
     3841 aattcggcgc ttcctgggtg atggccacgt catgggcgtg gctctcccgg aggcccgcgg
     3901 acgtgatccg cacgaagcgg ccgtacttct gcaactcctg gatggagcgg cagcccgtgt
     3961 accccatgcc cttccgcaag cccgccacga tctggccgag atagggcttg agctccccct
     4021 tgtaggggat gcggccctcg atgccctcgg gcacgggctc ctcgtccttg gccatctggt
     4081 accggtcccc ggctccgtcc ttgagggccc ccagggagcc catgccccgg tactccttgt
     4141 agatccggcc ctcgtacagg atctcccggc ccggggactc cttgagcccc gcgaagaggt
     4201 tcccgatcat gaccgaggaa gcccccgcgg ctacggactt gaccacgtcc ccgg
//
LOCUS       DAOTEI010000237         4227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_15388_length_4227_cov_5.721237, whole genome shotgun sequence.
ACCESSION   DAOTEI010000237 DAOTEI010000000
VERSION     DAOTEI010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15388_length_4227_cov_5.721237"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="P5313_15810"
     CDS             <1..246
                     /locus_tag="P5313_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HRY81881.1"
                     /translation="STMGNIAALEPAVQRQWIKALTQAVSAVQKQGFFPLILCSEAAR
                     ALVKSSTEREIPDLVVLSVPEVVADVSVEAIGEIRLE"
     gene            277..1467
                     /gene="flhF"
                     /locus_tag="P5313_15815"
     CDS             277..1467
                     /gene="flhF"
                     /locus_tag="P5313_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRY81882.1"
                     /translation="MEYFTEQGASHREAIERVRAKYGDAAQILTQRVVRTGGVLGFFA
                     REGVELTGYIRQEAAKEAANRKLDLEEEKRRLLASIQDRTLQEVLTEVRDLKRTVETY
                     RPPEAEADHPNLKALDDLLILNEFTEPYRRGILDKVRAEFTLDALEDWDGLQDAALEW
                     IGRDIAVVPGPASPGPRVFVLVGPTGVGKTTTIAKLAAIHKIGIGGLPPRDVRMITID
                     SYRIGAKQQIETYGDIMGVPTSCVETAEDLAKTIALYRDVDLILVDTIGKSPREAVKL
                     AEMKQILDACGPRAEVHLAMAATTKATDMEEILRQFEPFGYHAVVVTKLDETNRLGNV
                     ISVLSQRRKPVSYLTDGQKVPQDIQPAAVPRFLLGLEGFKLDRDRIEEKFGSRRETRA
                     EGRR"
     gene            1467..2348
                     /locus_tag="P5313_15820"
     CDS             1467..2348
                     /locus_tag="P5313_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRY81883.1"
                     /translation="MEDQAQKLRELMQAGAPSAAPQGASSQKRTRVITVTSGKGGVGK
                     TNLSVNLALAYAQQGKRVIVLDADLGLANVNIMLNMIPKYNLYHVIRKQKTMKEIILD
                     TDYGIQIVAGASGFSKIANLSEEERQSFISELYSLAGADIIIIDTSAGVSSNVLSFVA
                     AADDAIIITTPEPTAITDAYGIIKIIATEVENLNIGLKLVVNRVKSVLEGKKVAERII
                     NISGQFLNLKVEYLGCIYEDPIVPASVIRQRPFILSDPKSKAAISVHHLVSRIEKTDF
                     SDDKGIGRFIRKLFGRE"
     gene            2482..3132
                     /locus_tag="P5313_15825"
     CDS             2482..3132
                     /locus_tag="P5313_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81884.1"
                     /translation="MSSDIRIMSIAAGAAFLVSALTGIVSQVAFGALLLRAILSGAAF
                     AALGAGAAYVARVFLPELFGEPSHPDSAAPVPGIADAEHHLNIVLPGGDEAAEEVEEL
                     PEAVDPAGAGEDDLRNLEREAAEIGSDSLVPEEESREARAPVSARPPASFDDLDVLPD
                     LDGLSDVFSAASHEDIDAGEAPARDPISTTGASRDGIDAATMAQAVRTLLKRDQKG"
     gene            3139..3927
                     /gene="whiG"
                     /locus_tag="P5313_15830"
     CDS             3139..3927
                     /gene="whiG"
                     /locus_tag="P5313_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HRY81885.1"
                     /translation="MNNPLLEHTTEEELWVEYKRKRDPAIREAFIKQYAPLVKYVAGK
                     VASSMPHSVEFEDLVGFGVFGLIDAIDKFDPEKNVKFKTYAVTRIRGAIFDELRSIDW
                     VPRSVRQKTKELEEAIQQLESKLGRPASDMEIAHAMGVTEDELAKTMLKISGTAVLSL
                     NDVWYSGDDADKISIGDSIESPGSLNPDSMIEREEIRRVIVQAIQELPDKEKKVLVLY
                     YYEELTLKEIGQVLEVTESRVSQLHTKAILRLRAKLTNIRKGIV"
     gene            3930..>4227
                     /locus_tag="P5313_15835"
     CDS             3930..>4227
                     /locus_tag="P5313_15835"
                     /inference="COORDINATES: protein motif:HMM:NF026154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HRY81886.1"
                     /translation="MPGTVGLPQIQELMRRYLDEDREKTSVAAEGASLEEALRNAAIQ
                     LECPVASLDFEAVEKGVPGTLGLGRKPWKIRAYKTSVKKQVAAAESSFGEGILSE"
BASE COUNT          830 a         1324 c         1428 g          645 t
ORIGIN      
        1 tccacgatgg gcaacatagc cgccctggag ccggcggtgc agcggcagtg gatcaaggcc
       61 ttgacccagg cggtctcggc ggtccagaag cagggtttct tcccgctcat cctctgttcg
      121 gaggcggccc gggccctggt caagtcgagc accgagcggg aaatcccgga cctcgtcgtg
      181 ctctcggtgc ccgaggttgt ggcggacgtg agcgtggaag ccatcggcga gatacggctg
      241 gaataggccg gaccgaacat ggaagggaac gacgggatgg aatactttac cgaacagggg
      301 gcctcccacc gcgaagccat cgagcgggtg cgggccaagt acggggacgc ggcgcagatt
      361 ctgacccagc gggtggtccg gaccggcggg gtcctgggtt tcttcgcccg ggaaggggtt
      421 gagctcacgg gctacatccg ccaagaggcc gccaaggagg cggccaaccg gaaactcgac
      481 ctggaggagg agaagcggcg gctcctggca tccatccagg accggaccct ccaggaggtc
      541 ctcacggagg tccgggacct gaagcggacc gtggaaacct accgtccccc ggaggcggag
      601 gcggaccatc cgaacctgaa ggccttggac gacctgttga tcctgaacga gttcacggag
      661 ccctaccgcc gggggatcct ggacaaggtg cgggccgagt tcaccctgga cgccctggag
      721 gactgggacg gcctgcagga cgcggccctg gaatggatcg gccgggacat tgcggtcgta
      781 ccgggccccg cctccccggg cccccgggtc ttcgttttgg tggggcccac cggggtgggg
      841 aagaccacca cgatcgccaa gctcgcggct atccacaaga tcgggatcgg gggtcttccg
      901 ccccgggacg tgcggatgat caccatcgac agctaccgta tcggcgcaaa acagcagatc
      961 gagacctacg gggacatcat gggggtgccc acctcctgcg tggagaccgc ggaggacctg
     1021 gccaagacca tcgccctgta ccgggacgtg gacctgatcc tggtggacac tatcggaaag
     1081 agcccccggg aggcggtcaa gctggcggag atgaagcaga tcctggacgc ctgcggcccc
     1141 cgggcggaag tccacctggc catggccgcc acgaccaagg cgacggacat ggaggagatc
     1201 ctacggcagt tcgagccctt cggctaccac gccgtggtgg tcaccaagct ggacgagacg
     1261 aaccgcctgg ggaacgtgat ctccgtcctg tcccagcgac gcaagcccgt gtcctacctc
     1321 acggacggcc agaaagtgcc ccaggacatc cagcccgccg ccgttccccg gttcctcctg
     1381 gggttggagg gcttcaagct ggaccgcgat cggatcgagg agaagttcgg atcccgccgg
     1441 gagacccggg cggaggggag gcgttgatgg aagaccaggc acagaaactc cgggagctca
     1501 tgcaggcggg cgccccctcg gcggccccgc agggcgcctc ctcccagaag cggacccggg
     1561 tgatcacggt gaccagcggc aagggcgggg tggggaagac caacctctcg gtcaacctgg
     1621 ccctggccta cgcccaacag ggcaagcggg tcatcgtgct ggacgcggac ctggggttgg
     1681 ccaacgtgaa catcatgctg aacatgatcc ccaagtacaa cctctaccac gtcatccgca
     1741 agcagaagac gatgaaggag atcatcctgg acacggacta cgggatccag atcgtggccg
     1801 gggcttccgg gttctccaag atcgccaacc tctccgagga ggagcggcag agcttcatca
     1861 gcgagctcta ctccctggcc ggggcggaca tcatcatcat cgataccagc gcgggggtat
     1921 ccagcaacgt cctgtccttc gtggccgccg cggacgacgc catcatcatc accaccccgg
     1981 agcccacggc cattacggac gcctacggga tcatcaagat catagccacg gaggtggaga
     2041 acctgaacat cgggctcaaa ctcgtggtca accgggtgaa aagcgtcctg gaggggaaga
     2101 aggtcgcgga gcgtatcatc aacatctccg ggcaattcct gaacctgaag gtcgagtacc
     2161 tgggctgcat ctacgaggat cccatcgtgc cggcctcggt gatccgccag aggcccttca
     2221 tcctctcgga tcccaagtcc aaggccgcca tctcggtcca tcacctggtg agccggatcg
     2281 agaagacgga tttctcggac gacaagggca tcggccggtt catccggaag ctcttcggga
     2341 gggagtgagc gtgccgatcc gggaagggga cgggaaagtg cttcggcttg actccggctt
     2401 cggcgttgcc ataagatgga aggagatatc cgcaagccga gcacggagtc gcgggaacct
     2461 gcggggggag gggcgccagg ggtgagttcc gatatccgga tcatgtcgat cgccgccggg
     2521 gccgcgttcc tggtctccgc cttgacggga atcgtcagcc aggtcgcctt cggagccctg
     2581 ctgctgcggg cgatcctgtc cggcgcggcc ttcgccgccc tcggcgcagg tgcggcctac
     2641 gtggcccgcg tcttccttcc ggaactgttc ggagaaccct cccacccgga ctcggccgcg
     2701 cctgttccgg gaatcgcgga tgcggagcat cacctgaaca tcgtcctgcc cggcggggac
     2761 gaggccgccg aggaggtgga ggagttgccg gaagccgtgg atccggccgg ggccggagag
     2821 gatgacctga ggaacctgga acgggaagcc gcggagatcg gttcggacag tctcgtcccg
     2881 gaggaggagt cccgggaagc ccgggccccc gtttcggcgc ggcctcccgc ctccttcgac
     2941 gacctggacg tgctgccgga cctggacggg ctttcggacg tgttttccgc cgcgtcccat
     3001 gaggatatcg acgcgggcga ggctcccgcc cgggatccca tttccacgac cggggcttcc
     3061 cgggacggga tcgacgccgc gacgatggcc caggccgtga ggacgctgtt gaaacgggat
     3121 cagaaagggt aacccgagat gaataatccc ctgttggaac atacgaccga agaggaactc
     3181 tgggtcgagt acaagcggaa acgggatccg gcgatccgcg aggccttcat caagcagtac
     3241 gccccgctgg tgaaatacgt ggcagggaag gtcgcctcct ccatgcccca ttccgtggaa
     3301 ttcgaggacc tcgtcgggtt cggggtcttc ggcctcatcg acgccatcga caagttcgat
     3361 ccggaaaaga acgtcaaatt caagacttac gcggtcaccc gcatccgggg ggccatcttc
     3421 gacgagctcc gttccattga ctgggtaccc cgttcggtcc gccagaagac caaggagctg
     3481 gaggaggcca tccagcagct ggaatccaag ctgggccgcc ccgcctcgga catggagatc
     3541 gcccatgcca tgggggtcac cgaggacgaa ctggccaaga ccatgctgaa gatctccggt
     3601 accgccgtcc tttccctgaa cgacgtgtgg tattccgggg acgacgcgga caagatctcc
     3661 atcggagaca gcatcgagag cccgggcagc ctcaacccgg attccatgat cgagcgggag
     3721 gagatccgcc gagtgatcgt ccaggccatc caggaattgc ccgataagga gaagaaggtc
     3781 ctggtgctgt attactacga ggagctcacg ctcaaggaga tcggacaggt gctcgaggtc
     3841 acggaatccc gggtcagcca gctgcatacg aaggccatac tccggctccg ggccaagctc
     3901 acgaacatcc gcaaggggat cgtgtagaca tgccgggtac cgtcgggctt ccgcagattc
     3961 aggaactcat gcgccggtac ctggacgagg acagggagaa gacctccgtg gcggccgagg
     4021 gagcctccct ggaggaagcc ctccggaacg ccgccatcca gctggaatgc cccgtggcct
     4081 ccctggactt cgaggccgtc gagaaggggg tccccggaac cctgggactg ggccggaaac
     4141 cctggaagat ccgggcgtac aagacctccg tcaagaaaca ggtggcggcc gcggaatcgt
     4201 ccttcgggga aggcatcctg tcggagg
//
LOCUS       DAOTEI010000238         4198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_15531_length_4198_cov_4.822834, whole genome shotgun sequence.
ACCESSION   DAOTEI010000238 DAOTEI010000000
VERSION     DAOTEI010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15531_length_4198_cov_4.822834"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="P5313_15840"
     CDS             <1..163
                     /locus_tag="P5313_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HRY81887.1"
                     /translation="GVLITDHNVRDTLEITHRAYVINLGEILVSGDRQAILDSEVARK
                     IYLGEEFRM"
     gene            568..1338
                     /locus_tag="P5313_15845"
     CDS             568..1338
                     /locus_tag="P5313_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRY81888.1"
                     /translation="MQYFDTHAHIGLIFDDPIDQLLVCQQAKQAGVTRLISICNSLID
                     FAQVYENLKTAEHVYHAVGVSPSEVTTPGKDWRHRIDEGVKLPRVVAIGEIGLDYHHK
                     FGDRKSQIELFIEQLEIAARYDLPVIIHNREAGKDVLDVLKDRLPPAGGVLHCYSEDA
                     EYAMRAMDLNLYFSFAGNLTYRNARNLHDTVRVLPLERILVESEAPFMVPAEFRGKRN
                     KPEYLPSTVDFLAELLDIAVEDVAQATFDNASRFFKLA"
     gene            1564..2574
                     /gene="dusB"
                     /locus_tag="P5313_15850"
     CDS             1564..2574
                     /gene="dusB"
                     /locus_tag="P5313_15850"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRY81889.1"
                     /translation="MSCSEENEILSASLYHPVTVGTAVLPGNLFLAPVAGYSDAAFRS
                     VCAEHGCDLAYTEMVSSEALIRGHRATRTLLARGESERQYAVQIFGADPRSMARAAEL
                     LAPFHPTLVDINCGCPVPKIVRAGAGSALLRDPGRLAEIVRAVCSSSPAPVTVKIRLG
                     WDEGSVNFLETAAAAVEAGASAVTLHARTRAQGYSGKADWPALARLKSSLSVPVFGSG
                     DVFRPGDARRMIEETGVDGVMIARGAMGNPFIFRQAREVLEGREPGEPSPAERAAAAR
                     RHLTLAVEYLGERTACVEFRKQMCAYTKGVRGGAELRAAAVKCSTAAEYEDVFRLWDS
                     CA"
     gene            complement(2587..3834)
                     /locus_tag="P5313_15855"
     CDS             complement(2587..3834)
                     /locus_tag="P5313_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81890.1"
                     /translation="MTRLALAAAAALALASCSRDPNSAVIMTDSPEFAFYVDTYNASQ
                     DRYRLELQFRPNVPKALAQEAENPPALAVGRFLANDQARSRFVNLDYLFRELIVNPSG
                     VYQGLLQRGEFDGRQLLLPVSFNMPLVAWRRGEASPGKDNFVISLEEIRSAAAARNQL
                     AGSAFVRMGFSPRWDPDFLLLFARMSGAAFREGSPLSWNDEGLSASLERLRAWSSEVN
                     RSAADEDDFQFKYLYLPRNLSVQEGRIAFAYMDSSQFFLLSEERRAALEYRWIARETA
                     IPVLEEALYAGILRRGKGRAAAESFLKWFFREETQRYLLEKTRSLRTSETIFGIAGGF
                     SSIRSVNEKVLPVFYPSLLGHLPPPEYIEAPPTLPADWTDLAGRVLKPFLINATGTLS
                     PDFDPKSDLRRRMDQWKRQTAGT"
     gene            3940..>4198
                     /locus_tag="P5313_15860"
     CDS             3940..>4198
                     /locus_tag="P5313_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81891.1"
                     /translation="MPIPPGLPYHGRWNRYSSWLRARYGGPTARVPVDGGFSCPNRRP
                     DGSGGCSFCDSDGSRSPVLGSAGSVAEQADQAASFQRARYGA"
BASE COUNT          718 a         1378 c         1390 g          712 t
ORIGIN      
        1 cggcgtgctc atcacggacc acaacgtccg ggacaccctg gagatcaccc atcgggccta
       61 cgtcatcaac ctgggggaga tcctggtttc cggagaccgc caggccatcc tggactccga
      121 ggtcgcccgg aagatctacc ttggagaaga attccgaatg tagaactagc ctggaatcgg
      181 agaatcggac aggatgaaca ggattcggga aggatgaaca agataaagcc atatatagaa
      241 aagatatagt aattcacatt cgccttcctt tccccatctc ttatcttgta aatccttttt
      301 tctcctgtcc atcctgtcta gcatttcctg aatactgatt ctccgtgccc tccgtggtaa
      361 ggttcgataa aggcaagggg gatgacacac ccgggcgggt tcggtatact aattcccgta
      421 gattctccaa tctgtccgcc ccagacgccg tccggacccc cggtccccgg tttttccgtt
      481 tgagttgacg gctcggtggg tgtatgctac attctatcca cgtcctccta gaggatgcct
      541 catttcctta tcccgattgg aagccgaatg cagtacttcg acactcatgc ccatatcggg
      601 ctcatcttcg atgacccgat agaccagctc ctcgtctgtc agcaggccaa gcaggccggg
      661 gtgacccggc tcatcagcat atgcaacagt ctcatcgact tcgcccaggt ctacgagaac
      721 ctgaagaccg cggagcacgt ctaccacgcc gtgggggttt ccccgtcgga ggtgaccact
      781 ccgggcaagg actggcgcca ccggatcgac gagggcgtca agctcccgcg cgtcgtcgcg
      841 atcggggaga tcggcctgga ctaccaccac aagttcgggg accggaagag ccagatcgag
      901 ctcttcatcg agcagctcga gatcgccgcg cggtacgacc tgccggtcat catccacaac
      961 cgggaggccg gcaaggacgt cctggacgtg ctcaaggacc gccttccgcc cgccgggggc
     1021 gtgctccact gctactccga ggacgccgag tacgcgatgc gggcgatgga cctcaacctc
     1081 tacttctcct tcgccggcaa cctgacctac cggaacgcgc gcaacctgca cgacacggtc
     1141 cgggtcctgc ccctggagcg gatcctggtg gagtctgagg cccccttcat ggtgcccgcg
     1201 gagttccggg gcaagcggaa caagccggag tacctgccct ccacggtgga cttcctggcg
     1261 gagctcctgg acatcgcggt ggaggacgtg gcccaggcca ccttcgacaa cgccagccgc
     1321 ttcttcaagc tggcataggc attcaggaat caccacggag atcacggagc acacggagaa
     1381 gagagaggat cggagaagag agacaggata aaaaggatag ggaacggatc aacaggatag
     1441 aggttgagaa cggaacttcc atttctattc tccatcctgt ttatccatct aaatcctgtt
     1501 catcctgtct ttcctctcct ggaaggctgt tttctccgtc ctctccgtga ttcattcctg
     1561 tcaatgtcct gctccgagga gaacgagatc ctgagcgcat ccctgtatca tcccgtgacg
     1621 gtcgggacgg cggtcctgcc cggaaacctc ttcctggccc ccgtggcggg ctactccgac
     1681 gccgccttcc gctccgtctg cgcggagcac ggctgcgacc tggcctacac ggagatggtc
     1741 agctcggagg ccctgatccg gggccaccgg gctacacgga ccctcctggc ccggggcgaa
     1801 tccgagcggc agtacgcggt ccagatcttc ggggcggatc cccgatccat ggcccgggcc
     1861 gcggagctcc tcgcgccctt ccatccgacc ctggtggaca tcaactgcgg gtgcccggtg
     1921 ccaaagatcg tacgggccgg ggcgggctcg gccctcctgc gggatcccgg ccgcctggcc
     1981 gagatcgtcc gcgcggtgtg ctcctcctcc cccgccccgg tcacggtcaa gatccgcctg
     2041 ggctgggacg aggggagcgt gaacttcctc gagaccgcgg cggctgccgt ggaggccggg
     2101 gcctccgcgg tgaccctgca cgcccgcacc cgggcccagg gctattcggg gaaggcggat
     2161 tggcccgcac ttgcccggct caagtcgtcc ctctcggtgc cggttttcgg cagcggggac
     2221 gtcttccggc ccggggacgc ccggcgcatg atcgaggaga ccggcgtgga cggggtcatg
     2281 atcgcccggg gggccatggg gaaccccttc atcttccggc aggcccggga ggtcctggaa
     2341 ggccgggaac ccggggagcc gtccccggcg gagcgggcgg ccgcggcgcg ccgccacctg
     2401 accctggcgg tggagtacct cggggagcgg accgcctgcg tggagttccg caagcagatg
     2461 tgcgcctaca ccaagggcgt ccggggcggc gcggagcttc gggcggccgc ggtcaagtgc
     2521 tccaccgcgg cggagtacga agacgtcttc cggctctggg attcctgcgc ctgagggagc
     2581 cccggttcag gtgccggcgg tctgcctctt ccactggtcc atccgcctcc tcaggtccga
     2641 tttgggatcg aagtccgggg acagggtccc ggtggcgttg atcaggaagg gcttcagcac
     2701 ccgtccggcc agatcggtcc agtccgcggg gagggtcggc ggggcctcga tgtactccgg
     2761 cggaggcagg tgcccgagca gggaagggta gaagaccgga aggaccttct cgttcaccga
     2821 ccgtatcgag gagaagcccc ccgcgatccc gaagatcgtc tcgctggtgc gcagggaccg
     2881 ggtcttctcc agcaggtacc gctgggtctc ctcccggaag aaccacttca ggaaggattc
     2941 cgcggcggcc cgtcccttcc cgcgcctcag gatccccgcg tacagggcct cctccaggac
     3001 ggggatggcg gtctcccggg cgatccaccg gtactccagg gccgccctcc tctcctcgga
     3061 caggaggaag aactgggacg aatccatgta ggcgaaggcg atccgtccct cctggaccga
     3121 caggttgcgg ggcaggtaga ggtacttgaa ctggaagtcg tcctcgtcgg cggcggaccg
     3181 gttcacctcc gaggaccagg cccggagcct ctcgagggac gcggacaggc cctcgtcgtt
     3241 ccacgacagg ggggaaccct cccggaaggc ggcgccggac atccgtgcga acaggaggag
     3301 gaagtccggg tcccagcgcg gggagaagcc catccggacg aaggccgatc cggccagctg
     3361 gttcctcgcc gcggcggcgg agcggatctc ctccagggag atcacgaagt tgtccttccc
     3421 gggcgaggcc tccccgcgcc gccacgccac caggggcatg ttgaaggata cgggcaggag
     3481 gagttggcgg ccgtcgaatt cccctcgctg caaaagcccc tggtagaccc cggaggggtt
     3541 cacgatgagc tcccggaata ggtagtccag gttcacgaac cgggaccggg cctggtcgtt
     3601 ggccaggaaa cggccgaccg ccagggccgg ggggttctcg gcctcctggg ccagggcctt
     3661 gggcacgttg gggcggaact ggagctccag gcggtaccgg tcctgggagg cgttgtaggt
     3721 atccacgtag aaggcgaact ccggggaatc ggtcatgatc accgcggagt tggggtcccg
     3781 ggaacaggag gccagggcca gggcggcggc tgccgcgagg gctagacggg tcatggttac
     3841 acggtaccgg aaaggggcct tccgttcaag ccgaacgggc ccggtcgcgg attcccgggc
     3901 gccggagacg cggcccgcac ggtcggggta gaatgggcca tgccgatccc cccgggtctg
     3961 ccgtaccacg gccgctggaa ccgctactct tcctggctcc gcgcacggta cggaggcccg
     4021 acggcccggg tgcccgtgga cgggggcttc tcctgcccca accgccgccc cgacgggtcg
     4081 gggggttgct ccttctgcga ttccgacggc tcccgttccc ccgtactcgg ctccgcgggg
     4141 agcgtcgcgg agcaggcgga ccaggccgcc tccttccagc gtgcccggta cggggcgg
//
LOCUS       DAOTEI010000239         4191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_15570_length_4191_cov_5.075435, whole genome shotgun sequence.
ACCESSION   DAOTEI010000239 DAOTEI010000000
VERSION     DAOTEI010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15570_length_4191_cov_5.075435"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="P5313_15865"
     CDS             <1..536
                     /locus_tag="P5313_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81892.1"
                     /translation="RSLKEAVAEADRRVEVLRRESGRRVQEGAVYDRLGRLRASVSGA
                     SESAGAPRYPETAEPAARYAVPASSAPGIPPAGRPSPEDAEAPAVGRPSPSREYPGIA
                     PAEEAEPLQPSIPFVTFSSTPLRAKPPLKEEVLSLNRRGISSEFIAAKLGITVAEVEL
                     IVSLEEQRGRAGREGGT"
     gene            533..2200
                     /locus_tag="P5313_15870"
     CDS             533..2200
                     /locus_tag="P5313_15870"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRY81893.1"
                     /translation="MIRGILGIDFGTTNSLCAWIEDGRPQIVPNDRGSRTTPSVVALS
                     HSGEILVGESAKNQAVASPQTTLTGVKRRLGSRDPVRLGDRSFPPEEAASWIFAKLKR
                     DSEAFLGFPVEQAVVSVPARFGEAQRRAVKEAARRAGLEVPRLVNEPTAAAVARAFLD
                     PEEADTERVVLVYDFGGGTFDVTVLAARGRVCEVLSSAGDDRLGGMDIDALLYREAAE
                     AFRRDWGVDPDSDPFLAQQLMDLSERAKIELSSRSEVTFTIPFLNAGRGLSHPSFGLS
                     RARLEDLTRPLAERTLSLVRKALDEAGKRPMDVGALVLSGGSSRIPLVRRMISELLPL
                     EPEGRVNPEEIVAVGAAVEAAILSGRAGGFVVRDVNSRTYGVEIEGGRFVELLPRNEP
                     LPSRRRRVFTTVVDGQESVEIHILQGESGSAAENLSLGRFLLPGIRKAGKGEPKIQVE
                     FAIDESDLLRVRAMDLDTRTEQAVVVTGSGADGGDAEKLARLAARVRSLAGAEDIDNG
                     FRSEIEELLARAAWAVESGSEEDRRLARGSLEAVAGELLARKKVASP"
     gene            2197..2805
                     /locus_tag="P5313_15875"
     CDS             2197..2805
                     /locus_tag="P5313_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRY81894.1"
                     /translation="MKSDRELWAVFGLPPGADSRDLKKAFRQLTKRYHPDSSKDPATA
                     RRFSRVVRVYELLSRKAGPARETPAEPGGTDPEEDLFALGTEFTVSRDAVTRLEAVKR
                     LGLSGKRSAYIFLRKALYDDSPEVVAQAVRSVALLGIRQAEGEMASLFSRSGSALKRT
                     ILETARGTREPVFLSALRVARSDPDASIASDATEILGSLEGC"
     gene            complement(2825..4189)
                     /locus_tag="P5313_15880"
     CDS             complement(2825..4189)
                     /locus_tag="P5313_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY81895.1"
                     /translation="MKFTILVADDERNIREGLAEALRLEGYEVALAADGEEALKAVEE
                     GRIDLVVTDLRMPKVSGNEVLKRVAGSYPGMPVIVLTGHGTIEDAVDSMRVGAFDFIT
                     KPVNLDHLSMLIRRALESRELERQNQELQREIESQKRISSIIGRSPEMKKIFDLLRRV
                     APTKASVLITGESGVGKELIADALHNLSPRADKPFVKVHCAALAESLLESELFGHEKG
                     AFTGAAGRKRGRFELAHEGTLFLDEIGEINQNVQIKILRVLQERKFERVGGEETLEVD
                     VRVIAATNRDLKAEIEKGSFREDLYYRLNVVNIHVPPLRERKDDIPLLAGAFLREFTE
                     ENGKNLEGFDTKVRAAFFAYGWPGNVRELRNCVESAVVLARGSLVTLEDLPPGIREAQ
                     EDDTVRIPAGSSLAEAEKILIRETLAVQGGNKSRTAEILGIGRKTLYQKLQEYGLDGS
                     RAEE"
BASE COUNT          609 a         1426 c         1461 g          695 t
ORIGIN      
        1 tgcgttccct caaggaggcg gtggcggagg cggaccggag ggtggaggtc ctgaggaggg
       61 aatccggacg gagggtccag gagggggcgg tgtacgacag gctgggacgc ctccgtgcct
      121 ccgtttcggg cgcttcggag tcggccggtg cgccgcggta cccggaaacc gccgagccgg
      181 cggcccggta tgccgttccc gcgtcttccg ccccgggaat cccgccggcc ggtcggccga
      241 gcccggaaga cgctgaggcg ccggccgtcg gcaggccttc cccgagccgg gaataccccg
      301 ggatcgctcc ggccgaggag gcggaacccc tccagccctc catacccttc gtcacctttt
      361 cctccacgcc cctgcgggcc aagccccccc tgaaggagga ggtcctgtcc ctgaatcgcc
      421 gggggatctc ctcggagttc atagccgcca aactcggaat caccgtggcc gaagtcgagc
      481 tgatcgtgtc cctggaagaa cagaggggac gtgcgggccg ggaggggggg acgtgatccg
      541 ggggatcctg gggatcgatt tcggcaccac caattccctg tgcgcctgga tcgaggacgg
      601 tcgtccccag atcgtcccga acgaccgggg cagccggacc accccctccg tggtggccct
      661 gtcccattcg ggggagatcc tggtcgggga atccgccaag aaccaggccg tagcgtcccc
      721 ccagaccacc ctgaccggcg tcaagcgaag gctcggttcc cgggatccgg tacgcctcgg
      781 ggaccggagc ttcccgccgg aggaagccgc gtcctggatc ttcgccaagc tgaagcggga
      841 ctccgaggcc ttcctgggct ttcccgtgga gcaggccgtg gtgtccgtgc ccgcccggtt
      901 cggggaggcc caacggcggg ccgtcaagga agccgcccgc cgggcgggcc tggaggttcc
      961 ccggctggtg aacgagccga ccgcggcggc cgtggcccgg gccttcctcg accccgagga
     1021 ggcggacacg gaacgggtcg tcctggtcta cgatttcggc ggagggacct tcgacgtgac
     1081 cgtcctggcc gcccggggtc gggtctgcga ggtcctgtcc tccgccgggg acgaccgcct
     1141 ggggggcatg gacatcgacg ccctgctcta tcgggaggcc gccgaggcct tccgccggga
     1201 ctggggggtg gatcccgatt ccgacccctt cctggcccag cagctcatgg acctctcgga
     1261 acgggccaag atagagcttt cgtcccgctc cgaggttacc ttcaccatac cattcctcaa
     1321 cgcgggcagg ggcctgtccc atccctcctt cgggttgagc cgggctcgcc tggaagacct
     1381 gacccgaccc ctggcggagc ggaccctttc cctggtccgc aaggccctgg acgaggcggg
     1441 caaaaggccc atggacgtcg gcgccctggt gctctccggg ggctccagcc ggatccccct
     1501 ggtgcggaga atgatctcgg agcttttgcc cctggaaccc gaaggccggg tgaaccccga
     1561 ggagatcgtc gcggtcggcg cggcggtgga agccgcgatc ctctcgggcc gcgcgggcgg
     1621 attcgtcgtg cgggacgtga actcccgcac ctacggggtg gagatcgagg gaggtcggtt
     1681 cgtagagctc cttccccgga acgaacccct gccgtcccgg aggagacggg tgttcaccac
     1741 cgtcgtggac ggacaggaat ccgtggagat ccacatcctc cagggcgaat ccggatcggc
     1801 cgcggaaaac ctctccctgg ggcgcttcct cctgcccggg atccggaagg ccggcaaggg
     1861 ggaaccgaag atccaggtgg agttcgccat cgacgagagc gacctcctgc gcgtccgggc
     1921 catggatctg gacacccgca ccgagcaggc cgtggtcgtg acaggctccg gtgctgacgg
     1981 cggggacgcc gagaagctgg cccggctggc ggcccgggtc cggagcctgg cgggcgcgga
     2041 ggacatcgac aacggcttcc gatccgagat cgaggagctc ctggcccggg cggcctgggc
     2101 ggtcgagtcg ggttcggagg aggaccggcg cctggcccgg ggttccctgg aagccgtggc
     2161 cggggagctc ctggcgcgga agaaggtggc ctccccgtga aaagcgaccg ggagctctgg
     2221 gccgtattcg gcctgccgcc gggggcggat tcccgggacc tcaagaaggc ctttcgccag
     2281 ctcaccaagc ggtatcatcc ggattcctcc aaggatcccg ccacggcccg gaggttttcc
     2341 cgggtcgtgc gggtgtacga gctcctgtcc cggaaggcgg gtccggcacg ggaaaccccg
     2401 gcggaaccgg gaggaacgga tcccgaggag gacctgttcg ccctggggac cgagttcacc
     2461 gtgtcccggg acgcggtgac ccggctggag gcggtcaagc gcctggggct gtccgggaag
     2521 aggagcgcct acatcttcct gagaaaggcc ctgtacgacg attccccgga ggtggtcgct
     2581 caggccgtgc ggtccgtggc cctcctgggg attcggcagg ccgaggggga aatggccagc
     2641 ctcttctccc gctccggctc cgccctcaag cggaccatcc tggagacggc acggggcacc
     2701 cgggaaccgg tgttcctgtc cgccctgcgg gtggcgaggt cggatccgga cgcctccata
     2761 gcctcggacg cgacggagat cctcggctcc ctggagggct gctgacccgg gataccccgg
     2821 aacctcactc ctccgcccgg ctcccgtcca ggccgtactc ctggagtttc tggtagaggg
     2881 ttttccgccc gattccgaga atttccgcgg tgcggctctt gttccccccc tggacggcca
     2941 gggtttcccg gatcaggatc ttctccgcct ccgccaggga tgacccggcg gggatccgca
     3001 ccgtgtcgtc ctcctgggct tcccggatgc cgggggggag gtcctccagg gtcacgaggc
     3061 tcccccgggc caggacaacg gcgctctcca cgcagttgcg gagctcccgc acgttgccgg
     3121 gccagccgta ggcgaagaag gccgcccgta ccttggtgtc gaatccctcc aggttcttgc
     3181 cgttttcctc ggtaaactcc cgtaggaagg ccccggccag gagggggatg tcgtccttgc
     3241 gctcccgaag gggcgggacg tggatgttga ccacgttcag ccggtagtag aggtcctccc
     3301 ggaaagagcc tttctcgatc tccgccttca ggtcccggtt ggtggcggcg atcacccgga
     3361 catccacctc cagggtctcc tcgcccccga cccgttcgaa cttccgttcc tgcaggaccc
     3421 ggaggatctt gatctggacg ttctggttga tctccccgat ctcgtcgagg aagagggttc
     3481 cctcatgggc cagctcgaag cgcccccgtt tccgccccgc ggcccccgtg aaggctccct
     3541 tctcgtggcc gaagagctcg ctctccagga gggattccgc tagggcggcg cagtggacct
     3601 tgacgaaggg cttgtccgcc ctcggggaga ggttgtgcag ggcgtcggcg atgagctcct
     3661 tgccgacccc gctttccccg gtgatgagga cgctggcctt ggtgggcgcc acgcggcgaa
     3721 ggagatcgaa gatcttcttc atctcggggc tgcgcccgat gatggaggag atccgcttct
     3781 ggctctcgat ttcccgctgg agctcctggt tctgccgttc cagctcccgg ctttccaggg
     3841 cgcgccggat aagcatggac aggtggtcca ggtttacggg cttggtgatg aagtcgaagg
     3901 cccccacccg catggagtcc acggcgtcct cgatcgtccc gtggccggtc aggacgatga
     3961 cgggcattcc gggataggag cccgcgaccc gcttcaagac ctcgttcccg gagaccttgg
     4021 gcatacgcag gtccgtgacg accaggtcga tccgtccctc ctccacggcc ttcagggctt
     4081 cctcgccgtc cgcagccagg gccacctcgt agccctctag tcggagggcc tccgccagcc
     4141 cttcccggat gttccgctcg tcgtcggcga ccaggatggt gaatttcacg a
//
LOCUS       DAOTEI010000240         4189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_15578_length_4189_cov_5.436865, whole genome shotgun sequence.
ACCESSION   DAOTEI010000240 DAOTEI010000000
VERSION     DAOTEI010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15578_length_4189_cov_5.436865"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="P5313_15885"
     CDS             complement(<1..511)
                     /locus_tag="P5313_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY81896.1"
                     /translation="MNTEPLMEEKPATRADGMAALALNVVLTLASTAGTVLGIIALDR
                     GDGGFGALALTIACGLYASVIGPVLFGGLRILKPNEALVLTLFGRYHGTLRGPGFFFV
                     NPFSTAANPPDLEPTTGAVPGEGKPPSKPGSAAASAWGARKPARRISLKAMTLSNDKQ
                     KVNDALGNPI"
     gene            711..1580
                     /locus_tag="P5313_15890"
     CDS             711..1580
                     /locus_tag="P5313_15890"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRY81897.1"
                     /translation="MKLCLVVAEETVDQAAETLGRYDKVVPIFEVRADFLADLGPDSA
                     ALLRSSTDRSLIFTARLPADGGRWTAGPAARRRAYERAVAAGFDYVDLEIGAFPDLDT
                     GGRTRVIRSFHDMGGMPRNIREIFARACGNPGEIPKIAVTLNRPEDTVRFLEWNAELR
                     ASGPAPRVVTAMGRKGAFSRILGSRLGIEHTYVPVPGKLLLPGMVGLDAMTEGYGADR
                     IGDRTEVYGLVEPSSTYVPPETTDPEEDRPGTDPGADAVRVPFVLDSEEECRALRDFF
                     RIERFYSRNNRSS"
     gene            complement(1553..3673)
                     /locus_tag="P5313_15895"
     CDS             complement(1553..3673)
                     /locus_tag="P5313_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRY81898.1"
                     /translation="MSKSIDFSETPVTSLYGINSFSNAVMRERLPKNVYKELLEVQAG
                     EKELSLELAEVVASAMKDWALEKGATHYTHWFHPLTGLTAEKHDSFISPTPDGKILME
                     FSGKELIKGEPDASSFPSGGLRATFEARGYTAWDTTSPAFLKQDPTGTTLCIPTAFIG
                     YYGHALDKKVPLLRSMDALGAQALRVLRALGNETSRRVITTVGPEQEYFLVDSKYFER
                     RPDLMLTGRTVFGSMPAKGQEMEDHYFGAIKERVASFMREVNFELWKVGVSAKTQHNE
                     VAPNQFELAPIFTTTNVAVDGNQLVMETLQKVARRHGMEALLHEKPFAGVNGSGKHNN
                     WSMATDDGLNLLEPGENPESNARFLLFMTAVVEAVDRYAVLIRSAAATAGNDHRLGAN
                     EAPPAIISVFLGSQLCGILDAIVEGKAQSQSNGDKIKLGVTSLPSLPRDLSDRNRTSP
                     FAFTGNKFEFRMVGSSQSIATPNTYLNLAVAQVLSEFADRLEAASDRTAAIQAIVKDS
                     YARHRRVTFNGNGYSEEWVKEAERRGLPNIRASIETFGILTQPEIVSLFEGHKVLSKA
                     ELESRYHIYLEKYSKQINIEAGVMIDMVRRSIFPAATAFAGALARESASLAAIGAVSA
                     PQEKRAKRIAELTAEISEETEKLEKVLVEAQETEDALSQAKVYREKVTTQMSALRSRV
                     DAVEKLVAKDAWPLPTYEDLLFRL"
     gene            complement(4059..>4189)
                     /locus_tag="P5313_15900"
     CDS             complement(4059..>4189)
                     /locus_tag="P5313_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY81899.1"
                     /translation="GGNMAAAARSLGITERQMGLRVRHYAVNWRLYRAPRLSMKAT"
BASE COUNT          682 a         1311 c         1418 g          778 t
ORIGIN      
        1 tgatggggtt gcccagggcg tcgttgactt tctgcttgtc gttgctcagg gtcatggcct
       61 tgagggagat cctgcgggcc ggcttccggg ctccccaggc cgacgcggcc gcgctcccgg
      121 gcttggaggg aggcttgccc tcgccgggaa cggcccccgt cgtgggttcc agatccgggg
      181 gattggccgc cgtcgagaag gggttcacga agaagaaccc cggtccccgc agggtgccgt
      241 ggtacctccc gaaaagcgtc aggaccaggg cctcgttggg tttcaggatc ctgagccccc
      301 cgaagaggac gggcccgatc accgaagcgt acaggccgca cgcgatcgtc agggccaggg
      361 cgccgaagcc gccgtccccg cggtccaggg ctatgattcc caggacggtc ccggcagtcg
      421 aggccagggt caggacgaca ttcagcgcca gggcggccat gccgtccgcc cgggttgccg
      481 gtttctcctc catcaaaggt tcggtattca tgctatgcct cccgcccacc tgggcgatat
      541 gtttgtgata tcaatttgat agtatctcga tataagaaag tcgtcaaggg ttcctgggcg
      601 aatttcacgg aattcggccg atttccggcc tcgggccggg aggtccgggg agtaggccgt
      661 accgtagcgg ggaacttccc ttcccggccg ggatgtgctg tactgtcgat atgaagcttt
      721 gcctggtagt ggccgaggag accgtggacc aagccgccga aaccctgggc cgatacgaca
      781 aggtcgtccc gatcttcgag gtgcgcgccg atttcctggc cgacctggga ccggattccg
      841 cggcgctgct gcgaagcagc acggaccgga gcctgatctt cacggcccgg ctgcccgcgg
      901 acgggggccg gtggaccgcc ggtccggccg cccggcggag ggcctacgag cgggccgtcg
      961 ccgcgggctt cgactacgtg gacctggaga tcggcgcctt cccggacctg gataccggcg
     1021 ggcggactcg ggtgatacgg tccttccacg acatgggcgg gatgccccgg aacatccggg
     1081 agatcttcgc ccgggcttgc gggaatcccg gggagatccc caagatcgcg gtgaccctga
     1141 accgcccgga ggacacggtc cgattcctgg aatggaacgc ggaactccgc gcatccggcc
     1201 ccgctcccag ggtcgtcacg gccatgggcc gcaaaggggc gttctcacgg atcctgggga
     1261 gtcgcctggg catcgaacat acctacgtcc cggtccccgg gaaactcctc ctgcccggca
     1321 tggtgggcct cgacgccatg accgaggggt acggggccga ccggatcggc gaccggaccg
     1381 aggtgtacgg cctggtcgag ccgtcctcga cctacgtccc cccggagacg acggatcccg
     1441 aagaggaccg ccccgggacg gatcccgggg cggatgcggt ccgggtcccc ttcgtcctgg
     1501 actccgagga ggagtgccgg gccctccggg acttcttccg gatcgagcgg ttctacagcc
     1561 ggaacaacag gtcctcgtag gtcgggaggg gccaggcgtc cttggccacc agcttctcca
     1621 cggcgtcgac ccgggaacgg agggcggaca tctgggtcgt gaccttctcc cggtagacct
     1681 tggcctggga aagggcgtcc tcggtctcct gggcttcgac caggactttt tccagcttct
     1741 ccgtctcctc gctgatctcc gcggtcagct ccgcgatccg cttggccctc ttttcctggg
     1801 gagcgctgac cgccccgatg gccgccaggc tggcggattc ccgggccagg gcccccgcga
     1861 aggccgtggc cgcggggaag atggagcggc ggaccatgtc gatcatgacc cccgcctcga
     1921 tgttgatctg cttggagtac ttctccaggt agatgtggta ccggctctcc agctcggcct
     1981 tggacaggac cttgtggccc tcgaagaggc tcacgatctc cggctgggtc aggattccga
     2041 aggtctcgat ggaagcccgg atgttcggca ggccccggcg ctcggcctcc ttgacccatt
     2101 cctcggagta gccgttgccg ttgaaggtca ccctgcggtg ccgggcgtac gaatccttca
     2161 cgatggcctg gatcgcggcg gttctgtccg aggcggcctc cagcctgtcc gcgaactccg
     2221 agaggacctg ggccaccgcc aggttgaggt aggtgttggg ggtggcgatg gactgggagg
     2281 aaccgaccat gcggaactcg aatttgttgc ccgtgaaggc gaaggggctc gtgcggttgc
     2341 ggtccgagag gtcccgggga aggctgggaa ggctcgtgac ccccagcttg atcttgtccc
     2401 cgttgctctg ggactgggcc tttccctcca cgatggcgtc caggatgccg cagagctggg
     2461 agcccaggaa gaccgagatg atggccggag gggcctcgtt ggcccccagg cggtggtcgt
     2521 tgccggcagt ggccgcggcg gagcggatga gcaccgcgta ccggtccacc gcctccacca
     2581 cggcggtcat gaacaggagg aaccgggcgt tggactcggg gttctccccg ggttccagga
     2641 ggttgagccc gtcgtcggtg gccatggacc agttgttgtg cttgcccgag ccgttgaccc
     2701 ccgcgaaggg cttctcgtgg agcagggcct ccatcccgtg ccgtcgggcc accttctgca
     2761 gggtctccat gaccagctgg ttgccgtcca ccgccacgtt ggtggtggtg aagatggggg
     2821 ccagctcgaa ctggttcggg gccacctcgt tgtgctgggt cttggcggaa acccctacct
     2881 tccagagttc gaagttgacc tcccgcatga aggaggccac ccgctccttg atggccccga
     2941 agtagtggtc ctccatctcc tggcccttgg cgggcatgga cccgaagacc gtccgccccg
     3001 tgagcatgag gtccggccgc cgctcgaagt acttcgagtc cacaaggaaa tactcctgct
     3061 cgggccccac ggtggtgatg accctccggg aggtctcgtt gcccagggcc cggagcaccc
     3121 taagggcctg ggcgcccagg gcgtccatgg accttaggag ggggaccttc ttgtccaggg
     3181 cgtggccgta gtacccgatg aaggccgtgg ggatgcacag ggtcgtgccc gtcggatcct
     3241 gcttcaggaa ggccgggctg gtggtgtccc aggcggtgta gccccgggcc tcgaaggtgg
     3301 cccggaggcc tccggagggg aagctggagg cgtcgggctc gcccttgatc agctccttgc
     3361 ccgagaactc catcaggatc ttcccgtccg gggtcggaga tatgaaggaa tcgtgctttt
     3421 ccgcggtgag ccccgtgagg gggtggaacc agtgggtata gtgggtggct cccttctcca
     3481 gggcccagtc cttcatcgcg ctggccacca cctccgctag ctccagggaa agctccttct
     3541 cacccgcctg gacctccagg agttccttgt agacgttctt gggaagccgt tcccgcatga
     3601 ccgcgttgga aaaggaattg atcccgtaca ggctcgtcac gggggtctcg gaaaaatcga
     3661 tggacttgct catggtctct cctcgaagta tgggccggtc ccgcggagcc gcggacctgc
     3721 atatcccaat gcatagcagg agtcgtgccg gatcgggtgc ggatagaaga atatgtccga
     3781 cttcgggcag acttagcccc tttggaatga ataaacaggt agaaccacag agacacagag
     3841 gcacggagaa acgagggagg gaaattagga aagcaaggaa ccttataggc gggaacgcac
     3901 gattgagtta ctgtctttat gagtatagaa atactgggtt cgcctcaacc ctgcgcatcg
     3961 catccgaccg atcccggcga tcggcgtcgc cggaagcttt tccgagtctt cggcaggttt
     4021 tcctacataa ttgtatttag gagtaatatc tcctacattt atgtagcttt catggacaat
     4081 ctgggcgccc gatacaatct ccagttgacc gcgtagtgcc ggacccgcag acccatctgc
     4141 cgctccgtga tgcccaggga acgggccgcc gcggccatgt tgcctccgg
//
LOCUS       DAOTEI010000241         4181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_15623_length_4181_cov_4.092584, whole genome shotgun sequence.
ACCESSION   DAOTEI010000241 DAOTEI010000000
VERSION     DAOTEI010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15623_length_4181_cov_4.092584"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /gene="recN"
                     /locus_tag="P5313_15905"
     CDS             complement(<1..1583)
                     /gene="recN"
                     /locus_tag="P5313_15905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRY81900.1"
                     /translation="MLEELTIRDFALIDRVSVRFGPGLNLLTGETGAGKSILIGALGF
                     LLGGKADTGIIRSGAEETLVSGVLDVSGNPEARAWLSAHGMDCEDGTVVIRRSLKSTG
                     RGSVYIQNVPALRQDLADFTSLLVDVHGQHEHQSLLRPDSQRRLLDRFARIEAEVEAF
                     GERFTLLASRRREFEGMAASERERAREAELLAFAVDEISKAKLKPGEDAELEEEERLL
                     SQFEKLASAVEGARDLLADPSGGALPALRKVRTLLESAQAIDSKVSDLARRVDDGYYE
                     LEDVADTLRHYMDALRFSPERLEAVESRIAELQRLKKKYGDTLEEVIAYGADGAARLE
                     RLSNWEEDKAAREAEIAVLEREVYDRALAVSKARAAAGSDLEERIVAILRTLGMPGAR
                     FSIRLARKDSDGGKAVVGPNGIDEIDFLISPNRGEPLRELARIASGGEMSRVMLAVKT
                     ALADTDTIGTLIFDEVDAGIGGEVALSVGEHLHALAARKQVLCITHLASIAVRADTHY
                     RVEKAVQQDRTVTRLAALSG"
     gene            complement(1576..2433)
                     /locus_tag="P5313_15910"
     CDS             complement(1576..2433)
                     /locus_tag="P5313_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRY81901.1"
                     /translation="MQSDPGRALIIANLSKDDAGEVAREIGHFLRERGHTVDVHAFAG
                     KPDEVPRVAGADFAVSLGGDGTVLYSSRITAPWKVPVFPVNLGSLGFIAWIRKSEWRH
                     RLEDCLAGRLTLSERVMLDVEVVRDGRTEARFTALNEGVVSGSGSAKILDLRVVVSGA
                     PLCSYKSDGAIVATPTGSTAYSLAAGGPILEPDLDALVFNPICPFALSNRPLVLPGSE
                     EIEVNVAEKQRSSLILTLDGQDFFDLAPGDRVLFRRSESRARIWCAGRASFYDVLRHK
                     LNWSGGPDA"
     gene            complement(2445..3425)
                     /locus_tag="P5313_15915"
     CDS             complement(2445..3425)
                     /locus_tag="P5313_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY81902.1"
                     /translation="MDSVLSCMVEDAAEPGAFLSKFLRVAKEQLAFEQGAAFRSPAFA
                     LGAALDCFALPEGATVALSALSPAYYAYVLKDKRLNPLWLDIEPDTGCPDTEALAKAR
                     DGARVLLLFEGAGILPNPEEYVDLGIPVVEDVSESLGAFIGQTRAGSLGALTLLGLEQ
                     GGLATAGGGALVYANGRREAQALRNQADSLPPELLMTDLNAALGLSQIRALERTFERR
                     RELARLFEEALARSKHTALRLPGEGEPAYFGLPVVLPAGVKDVRAYARKKEVDTALAF
                     EGSCVARSLVPEGACPKAASLALRCLVFPLHPRIGKSAAQKVSRVLATLP"
     gene            complement(3478..3957)
                     /locus_tag="P5313_15920"
     CDS             complement(3478..3957)
                     /locus_tag="P5313_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY81903.1"
                     /translation="MSETESQIQLVTFQLSEELYGIDIMDVKEIVRIQEIRTIPNAPA
                     YVEGLYNLRGEIIPIINLHKRFHLKRAALGEDSELLSGFIIIEVAGMKLGVIIDKIER
                     VTPIEANEIQPPPQMLSGIGSEYIQGVVNQKSGYLIILDIRRLFNARELQKIEDLRK"
BASE COUNT          673 a         1383 c         1420 g          705 t
ORIGIN      
        1 ccggacaggg ccgccaggcg ggtgaccgtc cgatcctgtt ggacggcttt ttccaccctg
       61 tagtgagtat cggcgcgaac ggcgatggac gcaaggtggg tgatgcacag gacctgcttc
      121 cgggcggcca gggcgtggag gtgctccccc acggaaaggg cgacctctcc tccgatcccg
      181 gcgtcgactt cgtcgaagat cagggtcccg atggtgtccg tgtccgcaag ggcggtcttc
      241 acggccagca tgacccggct catctcgccc cccgaggcta tgcgcgcgag ttcccgcagg
      301 ggttcccccc ggttcgggga gatcaggaaa tcgatttcgt cgatcccgtt cgggccgacc
      361 acggccttgc ccccgtcgga atccttgcgg gccagccgga tcgagaaccg ggcgccgggc
      421 atgcccaggg tgcgcaggat ggccacgatc cgctcctcca ggtccgaacc cgccgcggcc
      481 cgggccttcg agacggcaag ggcccggtcg tagacctccc gctccaggac ggcgatctcg
      541 gcctcccggg cggccttgtc ctcctcccag ttcgagaggc gttccaggcg ggcggccccg
      601 tccgcgccgt aggcgatcac ctcttccagg gtatcgccgt acttcttctt gagcctctgg
      661 agctccgcga tacgggactc cacggcctcg agccgttccg ggctgaatcg gagggcgtcc
      721 atgtaatgcc gaagggtgtc cgcgacgtct tccagctcgt agtacccgtc gtccaccctc
      781 cgggcgaggt cggagacctt ggagtcgatg gcctgggccg actccaggag ggtccggacc
      841 ttccggagcg cgggcagggc ccctcccgac gggtcggcca ggagatcccg ggcaccttcc
      901 accgcggagg cgagtttctc gaactgggac aggaggcgtt cctcctcctc gagctccgcg
      961 tcctccccgg gcttgagctt ggccttggag atctcgtcca ccgcgaaagc caggagctcg
     1021 gcctcccggg ctcgctcccg ttccgaggcc gccatgccct cgaactcccg gcgccgggag
     1081 gcgagcaggg taaagcgctc gccgaaggcc tccacctccg cctcgatacg ggcgaagcgg
     1141 tccaggaggc ggcgctggga atccgggcgg aggagggatt ggtgctcgtg ctgcccgtgg
     1201 acgtccacca ggaggctcgt gaagtccgcc aggtcctgac gcagggcggg gacgttctgg
     1261 atgtagacgg atccccggcc cgtgctcttc agggacctgc ggatgacgac ggtgccgtcc
     1321 tcgcaatcca tgccgtgggc ggacaaccag gccctcgcct cggggttgcc cgagacgtcc
     1381 aggaccccgg aaaccagggt ctcctcggca ccggaccgaa tgatcccggt gtccgccttg
     1441 ccgcccagga ggaatcccag ggccccgatc aggatcgact tgccggcccc ggtctccccg
     1501 gtcaggaggt tgagcccggg accgaaccgg acgctgaccc ggtcgatgag ggcgaaatcc
     1561 cggatcgtca gctcctcaag catccggtcc cccggaccag ttgagcttgt gccgaaggac
     1621 gtcgtagaag gaggcccggc cggcgcacca gatgcgggcc cgggactcgg agcgacggaa
     1681 caggacgcgg tccccgggcg ccaggtcgaa gaaatcctgg ccgtcgaggg ttaggatcag
     1741 ggagcttctc tgcttttccg cgacgttcac ctcgatctcc tcgctccccg ggaggaccag
     1801 gggtcgattg gaaagggcga agggacagat cggattgaag accagggcgt ccaggtcggg
     1861 ctccaggatg ggcccccccg cggccaggga ataggccgtg gagcccgtcg gggtcgcgac
     1921 gatggcaccg tcggacttgt aggaacacag gggagccccg ctcacgacga cccggaggtc
     1981 caggatcttg gcgctcccgg atcccgagac gaccccctcg ttcagggccg tgaaccgggc
     2041 ctcggtccgt ccgtcccgga ccacctccac gtccagcatc acccgctccg agagggtgag
     2101 ccgcccggcc aggcagtcct ccaggcgatg cctccactcg cttttccgta tccaggcgat
     2161 gaaacccagg ctgccgaggt tgacggggaa cacggggacc ttccaggggg cggtgatccg
     2221 ggaggaatac aggacggtgc cgtcccctcc cagggagacc gcgaagtcgg ccccggccac
     2281 gcgggggacc tcgtcgggtt tgcccgcgaa cgcgtgcacg tccaccgtat ggccccgctc
     2341 ccgaaggaag tggccgatct cccgggctac ctccccggcg tcatccttgg acaggttcgc
     2401 gatgatcagg gcacggccgg gatcggactg cacgggtgcc tcctctaggg cagggtcgcc
     2461 aggaccctgg aaaccttctg cgccgcggac ttgcctatgc gggggtggag cgggaagacc
     2521 aggcaccgga gggccaggga ggccgccttc gggcaggctc cctcgggcac gagggaccgg
     2581 gcgacgcagg atccctcgaa ggccagggcc gtgtccacct ccttcttccg ggcgtaggcc
     2641 cggacgtcct tcacccccgc gggcaggacg acgggaaggc cgaagtacgc gggctcccct
     2701 tcccccggaa gccggagggc ggtgtgcttc gaccgcgcca gggcctcctc gaagagccgg
     2761 gccagttcgc ggcgccgttc gaaggtcctc tccagggcgc ggatctggga aagccccagg
     2821 gcggcgttca ggtccgtcat gagaagctcc ggaggcaggg agtccgcctg gttccggagc
     2881 gcctgggctt cccgccggcc gttggcgtag accaaggccc ccccgccggc cgtggccagg
     2941 cccccctgct cgagccccag cagggtcaag gctcccaggg agcccgcccg ggtctgtccg
     3001 atgaaggctc cgaggctttc cgagacgtcc tcgacgacgg ggatcccgag gtccacatat
     3061 tcctcggggt tcggaaggat tccggcaccc tcgaagagga ggaggacccg ggctccgtcc
     3121 cgggccttgg ccaaggcttc cgtatccggg cagcccgtat ccggctcgat atccaaccac
     3181 aaagggttca gacgcttgtc tttcagcacg taggcatagt acgccggaga gagcgcggac
     3241 agggcgaccg tcgctccttc gggcagggcg aagcagtcca gggcggctcc cagggcgaac
     3301 gcggggctgc ggaaggccgc tccctgctcg aaggcgagct gttccttggc tacgcggagg
     3361 aacttcgaga ggaaggctcc cggctccgcg gcgtcctcca ccatgcagga gaggacggaa
     3421 tccatatcct ttcgacggat gtaggaagaa cggacgggga tgatcactac ggttgtccta
     3481 ctttcttaga tcttcgatct tctggagctc ccgggcgttg aagagtcggc ggatatccag
     3541 gatgatgagg tatccgctct tctggttcac gaccccctgg atgtactccg agccgattcc
     3601 gctgagcatc tggggagggg gctggatttc gttggcctcg atgggggtca cccgctcgat
     3661 cttgtcgatg atgaccccga gcttcattcc ggccacttcg atgatgatga acccggacag
     3721 aagttccgaa tcctctccga gggccgcccg tttcaagtga aaccgcttgt gcaggttaat
     3781 gatcgggatg atctcgccgc gcagattata gagtccctcg acgtaggccg gcgcattcgg
     3841 gatcgtacga atctcctgga tgcgtactat ttccttcacg tccatgatgt cgattccgta
     3901 cagttcctcg ctcagttgga aggtcacgag ctggatctgg ctttccgttt cggacatgac
     3961 acccccgttt tagggcctgt agaggcaggc cggccgtctt ccgtggagtg tagcattttt
     4021 tgcccccggg agcaagtttc cggcgaatgc cgtgcggtac gcactgcatt cgcctcgcag
     4081 ttttcggcgt tctctaggag aacgccgaaa actgccgggc atgatagcgg ctcccggaac
     4141 ggtggacagt ggtcggtgga cggtgaacgg cgtggttgca g
//
LOCUS       DAOTEI010000242         4155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_15774_length_4155_cov_5.486829, whole genome shotgun sequence.
ACCESSION   DAOTEI010000242 DAOTEI010000000
VERSION     DAOTEI010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15774_length_4155_cov_5.486829"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /gene="ileS"
                     /locus_tag="P5313_15925"
     CDS             complement(<1..1737)
                     /gene="ileS"
                     /locus_tag="P5313_15925"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRY81904.1"
                     /translation="MYKPVDPKVSFPKMEEEVLAFWNEHRVFEKSVEQREGSPEYVFF
                     DGPPFATGLPHFGHFVPGTIKDIIPRYQTMKGRRVERRFGWDCHGLPVENLIEKELGL
                     NSKTDIEKFGIPEFNEACRASVLRYVKEWRTIMTRAGRWVDFDHDYKTMDPDYMESIW
                     WVVKALWDKGLIYEGHYILPYCPRCSTVLSNHELQLGGYKDVHDPAITVRFKVRSVPK
                     GFHDKTLADGSAYLVAWTTTPWTLPSNLALTLGPDVDYVCVRDGAERYVLAEARLSAY
                     YKDPAALEVLWKKKGSELKGIEYEPLFPYFEHTRAEGSFRTFNADYVTTEDGTGIVHT
                     APGFGEDDYQTLKGTGIPTVCPVDAECRFTGEVADFRGLFVKDADKPIIERLKAEGRL
                     VKREQYLHAYPHCWRCESPLIYRAVGSWFVKIDPVKPMMLDANSRIRWVPGHIQTGRF
                     GKWLENARDWAISRNRYWGNPLPIWKCDSCEATVCVGSRGELKELSGAEPVDLHKHFV
                     DGITWKCSCGGTMRRIPEVLDCWFESGSMPYAQVHYPFENKAHFEANFPADFICEGLD
                     QTRGWFYTLTILA"
     gene            2146..2706
                     /locus_tag="P5313_15930"
     CDS             2146..2706
                     /locus_tag="P5313_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81905.1"
                     /translation="MKKIIVALLALCLVFSVSAQGPKAGAGAGGGDAAPAGDRTGSET
                     APAYGAGEAARVMDRLQLKTEERERIRLMLEEQEGELVRVRAELREMQARMTRLMLEE
                     RVNRQEVERTLRQSMELEFRMRMIQAERNIRLKEMLGPERWANMYRLAQMVQAAERAG
                     KLEDFIAKSENPAQIRAMLQILQALF"
     gene            2712..3311
                     /locus_tag="P5313_15935"
     CDS             2712..3311
                     /locus_tag="P5313_15935"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY81906.1"
                     /translation="MNGDPGSAADRENVHRWNAGRDSEAFAALVRRYDPALRRVAAAV
                     LGSAARRDPGLLEDALQEGYRRMLDALRGYRAESEPFVFLAGVVRRACLDTLRKETRA
                     RGRTERAAVLGAAGGTEGTGRDPAGDAERAILADRVLAALDRLGDPDRSLVYLRDAEG
                     ASVAELADAFRMPEGTVKSRLARARTRLRGILAEEGVEP"
     gene            3308..3724
                     /locus_tag="P5313_15940"
     CDS             3308..3724
                     /locus_tag="P5313_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81907.1"
                     /translation="MRRDDVRDKALGALLRRAAETAAEASEKVRSGGALGYGAAHLEG
                     AVASPRHPRFSPGRFRVRIVGAAALAACLAAAIVVPALARFRADRELLSSAAEHLSRS
                     LIRESSSFLAELPAASELEAAAADWAGSLFRTDIDL"
     gene            complement(3947..>4155)
                     /locus_tag="P5313_15945"
     CDS             complement(3947..>4155)
                     /locus_tag="P5313_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81908.1"
                     /translation="LSWSAADYSTLALSALGDLEAGLFAATLTWAWDLAQNGGLTTYL
                     RLSNGQAGTEQWTGQAGVQLEVKF"
BASE COUNT          692 a         1298 c         1445 g          720 t
ORIGIN      
        1 ggccaggatg gtcagggtgt agaaccatcc ccgggtctgg tccagccctt cgcagatgaa
       61 gtccgcgggg aagttggcct cgaaatgggc cttgttctcg aaggggtagt gtacctgggc
      121 gtagggcatg gagcccgact cgaaccagca gtccaggacc tcggggatgc ggcgcatcgt
      181 cccgccgcag gagcacttcc aggtgatccc gtccacgaag tgcttgtgca ggtccacagg
      241 ctcggctccg gagagttcct tgagctcccc acggctcccc acgcacaccg tcgcctcgca
      301 ggagtcgcac ttccagatcg gaagggggtt gccccagtag cggttgcggc tgatggccca
      361 gtcccgggcg ttctccagcc acttgccgaa gcggcccgtc tggatgtgcc ccgggaccca
      421 gcggatccgg gagttggcgt ccagcatcat gggcttcacg ggatcgatct tgacgaacca
      481 ggatcccacg gcccggtaga tcagggggct ctcgcagcgc cagcagtggg ggtaggcgtg
      541 taggtactgc tcccgcttca cgagccggcc ctcggccttg agcctctcga tgatgggctt
      601 gtccgcgtcc ttgacgaaga ggccccggaa gtccgccacc tccccggtga agcggcactc
      661 cgcgtccacg gggcagaccg tggggatccc cgtgcctttc agggtctggt agtcgtcctc
      721 cccgaagccc ggggcggtgt gcacgatccc cgtgccgtcc tcggtggtga cgtagtcggc
      781 gttgaaggta cggaaggagc cctccgcccg ggtatgctcg aagtagggga acagcggctc
      841 gtactcgatg cccttgagct cggagccctt cttcttccag aggacctcca gggccgcagg
      901 gtccttgtag tacgcggaaa ggcgcgcctc cgccaggacg tagcgttccg ccccgtcccg
      961 gacgcagacg tagtccacgt ccgggcccag ggtcagggcc aggttggagg gcagggtcca
     1021 gggggtggtg gtccaggcga ccaggtaggc cgaaccgtcc gccagggtct tgtcgtggaa
     1081 gcccttcggc accgagcgga ccttgaaccg caccgtgatg gccggatcgt ggacgtcctt
     1141 gtagcccccc agctggagct cgtggttgga aaggaccgtg gaacagcggg ggcagtaggg
     1201 caggatgtag tggccctcgt agatgaggcc cttgtcccag agtgccttga ccacccacca
     1261 gatggactcc atgtagtccg ggtccatggt cttgtagtcg tggtcgaagt cgacccagcg
     1321 ccccgcccgg gtcatgatgg tccgccattc cttgacgtac cggagcaccg aggcccggca
     1381 ggcctcgttg aactcgggga tgccgaactt ctcgatgtcc gtcttggaat tgaggcccag
     1441 ctccttctcg atcaggttct ccacgggaag gccgtggcag tcccagccga agcggcgctc
     1501 cacccgtctg cccttcatgg tctggtagcg agggatgatg tccttgatgg tccccggtac
     1561 gaagtgtccg aagtgcggaa gtccggtggc gaaggggggg ccgtcgaaga agacgtactc
     1621 cgggctgcct tcgcgctgtt caacggactt ctcgaagacg cggtgctcgt tccagaaggc
     1681 gaggacctcc tcctccatct tcgggaagct gaccttggga tccacgggct tgtacacgat
     1741 gctcctccag gtatgttcga gaagggtccg tggaccgggg ggccgcggac cgcgcggagg
     1801 agtatgccat acaacgcacg ggtccgtaaa ctgcgcgggc gattggatct ttccggaatc
     1861 gaccacggag atcacggagg gcacggagga gggaatgaat aaggctctgg aaccatagat
     1921 ccaacaggat tcacaagata ggacaagatt aacaagatgg ggatatgaaa tacgattcat
     1981 cgattgatcg tatcttgttc atccatactc aatcatgttt atcttgttcg ataggtggtt
     2041 ctaaggcctt ttctccgtgg tctctgtgac ctccgtggta aaatcctggc aactatttat
     2101 gcaaccgaac cggcctccgc ggcgacctgt aggcaggagg atgatatgaa gaagatcatc
     2161 gtggcgttgc tggcgctttg tcttgtattc tccgtgtcgg cccaggggcc gaaggcgggc
     2221 gcgggcgcgg gcgggggcga cgccgccccc gcgggcgacc ggacgggttc cgagaccgcc
     2281 ccggcctacg gcgccggcga ggcggcccgg gtaatggacc ggctccagct caagaccgag
     2341 gagcgggaac ggatccggct gatgctggag gagcaggaag gggagctggt ccgggtccgg
     2401 gcggagctcc gggagatgca ggcccggatg acccggctca tgctggagga gcgggtgaac
     2461 cggcaggagg tggagcgcac tctccggcag agcatggagc tggaattccg gatgcggatg
     2521 atccaggctg agcgcaacat ccggctcaag gagatgctgg gaccggagcg ctgggccaac
     2581 atgtaccgcc tcgcccagat ggtccaggcg gccgagcggg cgggcaagct ggaagacttc
     2641 atcgcgaagt ccgagaaccc cgcccagatc cgggccatgc tccagatcct gcaggccctg
     2701 ttctgagccg aatgaacggg gatcccggtt ccgcggcgga ccgcgagaac gtgcaccggt
     2761 ggaacgccgg tcgggattcc gaggccttcg cggccctggt acggcggtac gatcccgccc
     2821 ttcggcgggt ggccgccgcc gtcctgggtt ccgcggcccg gcgcgacccg gggctcctgg
     2881 aggacgccct ccaggagggc taccgaagga tgctggacgc cctccgggga taccgcgccg
     2941 aatcggagcc cttcgtgttc ctggccgggg tggtgcgccg ggcctgcctg gataccctgc
     3001 gaaaggaaac ccgggctcgg ggccggaccg agcgggccgc ggttctcgga gccgccggcg
     3061 ggacggaggg aaccgggcga gaccctgccg gggacgccga gcgggcgatc ctggcggacc
     3121 gcgtcctggc cgccttggac cgcctggggg atccggaccg cagcctggtc tacctccggg
     3181 acgcggaagg ggcgtccgtc gcggaattgg cggacgcctt ccggatgccg gagggcaccg
     3241 taaagagccg tctggcccgg gcgcgaaccc ggctccgcgg gatcctcgcg gaggaaggag
     3301 tggaaccatg agacgggatg acgtgcggga caaggccctc ggcgccctgc tccggcgggc
     3361 cgcggagacg gccgccgaag cgtccgagaa agtccggtcg ggcggagcgc tcggctacgg
     3421 ggcggcgcac ctcgaaggcg cggtcgcatc cccgcggcat ccccggttca gcccggggcg
     3481 gttccgcgtc cggatcgtcg gggcggccgc cctggccgcc tgcctggccg cggccattgt
     3541 ggttcccgcc cttgcccggt ttcgggcgga ccgggagctc ctcagttcag ccgcggagca
     3601 cctctcccgg tccctgatcc gggaatcctc cagcttcctg gccgaactcc cggcggcgtc
     3661 cgaactcgag gccgcggccg cggactgggc gggatccctc ttccggacgg acatcgacct
     3721 ctgacggccc gcgaccggag tatgttttct tgaatcaacc acggagagca cggagcgcac
     3781 ggaggatgaa gccagtctgg ataggaacga caggatcaac aagatatccg tcataattga
     3841 ttttatctaa cttctatcct gtcaatcctt tcgtacatcc tgttatcctg tcctcccgta
     3901 tcattctccg tgttctccgc ggttaaatcc ggaaatggtg ctttcgtcag aatttgactt
     3961 ccagctgcac ccccgcctgg ccggtccatt gttcagtccc cgcctggccg ttggaaagcc
     4021 ggaggtaggt cgtgaggccc ccgttctggg ccaggtccca ggcccaggtc agggtggccg
     4081 cgaagagtcc ggcttccagg tcccccaggg cggacaatgc cagggtgctg tagtccgcgg
     4141 cgctccagga gaggg
//
LOCUS       DAOTEI010000243         4060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_16289_length_4060_cov_5.522597, whole genome shotgun sequence.
ACCESSION   DAOTEI010000243 DAOTEI010000000
VERSION     DAOTEI010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16289_length_4060_cov_5.522597"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /gene="thrS"
                     /locus_tag="P5313_15950"
     CDS             complement(<1..1288)
                     /gene="thrS"
                     /locus_tag="P5313_15950"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRY81909.1"
                     /translation="MSHPVETVRHSLSHVMAEAVVKLHPGTKVGIGPAIEDGFYYDFQ
                     FPQPLQPDDLPAIEKEMRRIIAGGASFVRREVSKEEAKNLFADEPFKLELIEGLADGT
                     ISVYEQGAFRDLCRGPHVDSTRDLRPDAFKLRSIAGAYWRGDEKRPMLTRIYAYAFGS
                     KADLEAHLKMLEEAEKRDNRRLGKELDLFSTHDEAGPGLIYWHPKGGRFRVALENWWR
                     EEHYQNGYEILFTPHIGKSWLWETSGHLGFYKAGMYSSMKIDEDDYYIKPMNCPFHIM
                     IYKNGVHSYRDLPLRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIICTPEQIEDEI
                     LEVLRFSLYMHKTLGFKDIKAYLATKPKEGAVGDDTRWAQALESLRKAVDREGLAYEM
                     DEGGGAFYGPKIDLKVKDAIGREWQLTTIQFDFNEPE"
     gene            complement(1802..2689)
                     /locus_tag="P5313_15955"
     CDS             complement(1802..2689)
                     /locus_tag="P5313_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY81910.1"
                     /translation="MTETVKDGVKGYLFALAATAFWSGNHFIARALRESIPPVSLAFL
                     RWAAATLAFLPFALPGAVRERRAVLRSLPYLALTALLGVSLFNTLIYYAGRTTTALNL
                     SLISLIFPVIILLLSRIFLGERMPLRRLAGIAVVLAGVAVLVTKGRLDALRSIGFAPG
                     DLLMLLAALVFSVYTLLLKRKPEGLSLSTLQFATFALGTLMLAPAFAWERASAPPVAW
                     TPALLGAVLYVGVFASLAAFVSWNQAIERIGTAKAGLVYYTLPIWSGLLAWVFLGEAP
                     TAAQAFSMALVVGGIVVAR"
     gene            2815..3120
                     /gene="clpS"
                     /locus_tag="P5313_15960"
     CDS             2815..3120
                     /gene="clpS"
                     /locus_tag="P5313_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRY81911.1"
                     /translation="MEESTEGEVLVREDEEIQEPEEFRVILLNDDYTTMEFVVAVIMT
                     VFHKGLAEATRIMLDVHKKGRGVVGVYPFDIAATKVDQVHTLARDNGYPLKCTMEKA"
     gene            3127..>4060
                     /locus_tag="P5313_15965"
     CDS             3127..>4060
                     /locus_tag="P5313_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY81912.1"
                     /translation="MKVNQEVQAIFNAAYNEAKIRSHEYLTPEHILYAALSFEQIRTI
                     LESCEADVEHIKQGMESYFEQKIPVVKNQEPIQTAGFQSVIERAVLQSQSAGKDEVRV
                     ADVLVSLYDEERNYSAYYLRKAGVKRLQLLDIISHGLDEESLGEYAPEPEAKDEEPGE
                     TEDSAAPDAEDEDEDAEPAPSRRGTARPGALERFTSDLTKLAREGRLEPVIGREAEIE
                     RTVQVLCRRLKNNPIHVGDAGVGKTAVTEGLAQRIVEGKVPPRLRDYTIHSLDMGSLL
                     AGTKFRGDFEERVKRVVDELLKKDRVILFIDEIHT"
BASE COUNT          744 a         1277 c         1387 g          652 t
ORIGIN      
        1 gctcgggctc gttgaagtcg aactggatgg tggtgagctg ccactcccgg ccgatggcgt
       61 cctttacctt gaggtcgatc ttgggaccgt agaaggcccc gcccccttcg tccatctcgt
      121 aggccaggcc ctccctgtcc accgccttgc gcagggattc caaagcctgg gcccagcggg
      181 tgtcgtcccc gaccgcccct tccttgggct tggtggccag gtaggccttg atgtccttga
      241 accccagggt cttgtgcatg tacagggaga agcgaaggac ctcgaggatc tcgtcctcga
      301 tctgttcggg ggtgcagatg atgtgggcgt cgtcctgggt gaagccccgg acccgcatga
      361 ggccgtggag ggtccccgac cgctcgtagc ggtacacggt cccgagctcg gcccagcgca
      421 ggggcaggtc ccgatacgag tggaccccgt tcttgtagat catgatgtgg aaggggcagt
      481 tcatcggctt gatgtagtag tcgtcctcgt cgatcttcat ggacgagtac atccccgcct
      541 tgtagaagcc caggtgcccc gaggtctccc agagccagct cttgccgatg tggggggtga
      601 agaggatctc gtagccgttc tggtagtgct cctcccgcca ccagttctcc agggccaccc
      661 ggaaacggcc ccccttgggg tgccagtaga tcagcccggg acccgcctcg tcgtgggtgg
      721 agaagaggtc gagctccttg cccaggcggc ggttgtcgcg cttctccgcc tcctccagca
      781 tcttgaggtg ggcttccagg tccgccttgg acccgaaggc gtaggcgtag atccgggtga
      841 gcatggggcg cttctcgtcg ccgcgccagt acgccccggc gatggagcgg agcttgaagg
      901 cgtcgggacg gaggtcccgg gtggagtcca cgtggggacc ccggcagagg tcccggaagg
      961 cgccctgctc gtacacggag atggtgccgt ccgcgaggcc ctcgatcagc tccagcttga
     1021 agggctcgtc ggcgaagagg ttcttggcct cctccttgct cacttccctg cggacgaagg
     1081 aggccccgcc cgcgatgatg cgccgcatct ccttctcgat ggccgggagg tcgtccggct
     1141 ggaggggctg ggggaactgg aagtcgtagt agaatccgtc ctcgatggcg ggtccgatgc
     1201 cgaccttggt tccgggatgg agcttgacga cggcttccgc catgacgtgg gacagggagt
     1261 ggcggacggt ttcgacggga tgggacatac ggtactcctc gggcgggaaa gcgttccggg
     1321 cgcgtcctgg cgcctccggg atcccttccc ggcgttctgg cgtaaggacg aatccccgaa
     1381 aagccctcga agaaggcccc cgcccccggc gacgccggac gcggtctcgc gaatccgcgg
     1441 taccacctta cttcggaccc cgccaccggg cggaatccgc gctcgaaacg ctgtgacggg
     1501 cgtccccggc ttccattacg gacccgtcgg atcgggggct cgctcatcgc gcgccccgat
     1561 tcctccgggt ggttcacgga agcggctccg gagtggtctt cggcgggtcc gcgacggggc
     1621 cctctcagcc gcggggcgcc ctctctcggg acgcggcggt ccgcctactc gtctccatca
     1681 aagcctgtgg ggtaaatatg gcgacggaag tggcggttgg tcaaggtagt gaatacgtga
     1741 atagtgaata tgtgaatagg gcgcgcccat tcacccattc acccattcac agcaagacta
     1801 cctacctcgc caccacgatc ccccccacga ccagggccat gctgaaggct tgggccgccg
     1861 taggggcctc ccccaggaag acccaggcca aaaggccgct ccagatcggc agggtgtagt
     1921 aaaccagccc ggccttggcg gtcccgatcc tctcgatagc ctggttccag gacacgaagg
     1981 ccgccaggga ggcgaagacc cccacgtaga ggacggcccc cagaagcgcc ggagtccagg
     2041 ctacgggcgg ggccgaggcg cgctcccagg cgaaggcggg agccagcatc agggtgccca
     2101 gggcgaaggt ggcgaactgc agggtggaaa ggctcaagcc ctcgggtttt cgcttgagca
     2161 gcagggtgta gaccgagaag accagggcgg ccaggagcat gagcaggtcc ccgggggcga
     2221 atccgatgga ccggagggcg tccaggcggc ccttggtgac taaaaccgcc accccggcca
     2281 ggaccacggc gatccccgcc aggcggcgga ggggcatccg ttcccccagg aagatccggg
     2341 agagcaggag gatgatgaca gggaagatca gggagatcag ggagaggttg agggccgtgg
     2401 tagtacggcc cgcgtaatag atcagggtat tgaagaggct gacccccagc agggccgtga
     2461 gggccaggta gggcaggctc cgcaggaccg cccggcgctc ccggacggct ccgggcaggg
     2521 cgaagggcag gaaggcgaga gtcgcggcag cccaccggag gaaggccagg ctcaccgggg
     2581 ggatcgactc cctaagggcg cgggcgatga aatggttgcc ggaccagaag gccgtggccg
     2641 ccagggcgaa gaggtaccct ttgactccat cctttaccgt ttcggtcatg ctttcccccc
     2701 gacgcgcatc ctaccggatc gtccgggctt cttcaagacc gggaagccgg gccgcccccc
     2761 ggcggcagtt gtttttttcc ccccctctgg gtatgttaat agtccgaagc ttatatggaa
     2821 gagtcgacag agggcgaggt ccttgtccgg gaggacgaag agatccagga gcccgaggaa
     2881 ttccgggtca ttctgctcaa cgacgactac acgaccatgg agttcgtcgt ggcggtgatc
     2941 atgacggtct tccacaaggg cctggcggaa gccacgagga tcatgctgga tgtccacaag
     3001 aagggtcgag gcgtcgtggg ggtgtatccc ttcgacatcg cggccaccaa ggtggatcag
     3061 gtccacactc tggcccggga caacggatac cccctcaagt gcacgatgga gaaggcgtaa
     3121 gggaccatga aagtcaacca ggaagttcag gccatattca acgcggccta taacgaggcg
     3181 aagatccgga gccacgagta cctgaccccg gagcacatcc tctacgcggc cctctcgttc
     3241 gagcagatcc gcaccatcct cgagtcctgc gaagcggacg tggaacacat caagcagggg
     3301 atggagtcct acttcgagca gaagatcccg gtggtcaaga accaggagcc catccagacc
     3361 gccggattcc agagcgtgat cgaacgggcg gtcctccagt cccagtccgc gggcaaggac
     3421 gaggtccggg tcgcggacgt cctggtttcc ctgtacgacg aggaacggaa ctactccgcg
     3481 tactacctcc gcaaggcggg ggtcaagcgc ctccagctat tggacatcat ctcccacggc
     3541 ctggacgagg aatccctggg cgagtacgct ccggagccgg aagccaagga cgaggagccc
     3601 ggggagaccg aagactccgc cgccccggac gcggaggacg aggacgagga cgccgaaccg
     3661 gccccgtccc gccgcggcac cgcgcgcccg ggcgccctgg agcgtttcac ctcggacctg
     3721 accaagcttg cccgtgaggg ccgcctggaa cccgtgatcg gccgggaggc ggagatcgag
     3781 cggaccgtcc aggtcctttg ccgccgcctc aagaacaacc caatccacgt gggggacgcg
     3841 ggggtcggga agaccgcggt caccgagggc ctcgcccagc ggatcgtgga ggggaaggtc
     3901 cccccgcggc tccgggacta caccatccat tccctggaca tgggatccct cctggcgggc
     3961 acgaagttcc ggggggattt cgaggagagg gtcaagcgcg tggtggacga gctcctcaag
     4021 aaggatcggg tcatcctatt catcgacgag atccacacca
//
LOCUS       DAOTEI010000244         4023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_16493_length_4023_cov_4.653730, whole genome shotgun sequence.
ACCESSION   DAOTEI010000244 DAOTEI010000000
VERSION     DAOTEI010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16493_length_4023_cov_4.653730"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="P5313_15970"
     CDS             complement(<1..136)
                     /locus_tag="P5313_15970"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRY81913.1"
                     /translation="MTSYKELGLVNTVELFKKAVSGGYALPAYNFNNMEQLQAIVQAC
                     V"
     gene            280..1176
                     /locus_tag="P5313_15975"
     CDS             280..1176
                     /locus_tag="P5313_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81914.1"
                     /translation="MDGNRGPRSVRCRISLLLALSGLLASCSRPVGLVLIDPALPVLD
                     PEGAKAFSSFRVPGTRRAVRDYPVSESAAFFGGLSAPPPSFVLLTPLLAPEYPAVRDA
                     FPEAVILGTGIPAGERSLSASWDPVPAAREAGVRAAAFLRNRGEGLSEPPEAVILVSG
                     AAGGGTGSGMAAEAFREGLLSARPDTPLRILELPGQAGEADVLIRGLGGPGAKAAFLD
                     PGTGGFRAARLLAESTPEGVRGPRFTVFRSALPPPPGTPADLLLLRETRDLLRAFRAA
                     LKEGRTGAIDVPEALRIPDRVP"
     gene            1282..2064
                     /locus_tag="P5313_15980"
     CDS             1282..2064
                     /locus_tag="P5313_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HRY81915.1"
                     /translation="MKRRVSRSICLAILLVSLLSAGGVFAQEKPVNLESKVIDSFDDP
                     EGNPWFVMGSKFSTAEFPKVASVNSWPISIYGYNPADKDKLKTLGVAMLFDRKEYNWV
                     DVVPGKKTGSGDSVKYEPQERNLPGRVAMLDMWIWSGNFNYYIEAFIRDYKGIVHTIY
                     MGDLNHVGWKNFRINIPSNIPQSKKYLPKLEGLKLVKFRIWTRPTEVVAIPAGAEEPA
                     HKKAIYFYFDQLKVLTDTYEDMYDGDALTSPELIEQTWSSGK"
     gene            2077..2814
                     /locus_tag="P5313_15985"
     CDS             2077..2814
                     /locus_tag="P5313_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HRY81916.1"
                     /translation="MKKIILIALALCLAAFASAQTAVGDPNPNDIGVDTAQQKLKEVS
                     VDKFEAAGFWKAFISPDEGAIQYRLFEGSPAGKANEPIADERYVGIDPKVADKFVLGV
                     RTDFFRRGYNEIRILADKPIPIEGISKTVSVWVAGRNLNHVLKIMILDAFNTPFELTV
                     GKLNFQGWKKLTVAIPPQNMDGKTGIIQRNFHFISRMGIKIVGFKIECDPMEAFGTYY
                     FYMDDLRAVTDLFAEDYRDPDDMKDTW"
     gene            2874..>4023
                     /locus_tag="P5313_15990"
     CDS             2874..>4023
                     /locus_tag="P5313_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY81917.1"
                     /translation="MNDYYEFLRRIFFFKDLSDQEILLVANACHEERHTSGDILFLEG
                     SAADRCYIVLEGRVEVWKDYNEAKPSLLGVHGPGHFFGELALLDELPRSATVVARENA
                     RVLFLYREDFRNLIRDHSSIALSVMVSISFMLRSSNEVFLEDLKKRNTELESAYRELE
                     EVHADRLRNERLSTLGKFSSMILHDIRNPLSMLKGLLQLMILNLHEPEKLEGHVRNAL
                     AETARLENLASEFLDYSRGEIRLNLGVTTAAWLFKRVEESVRERFRTSGLSVVTDCRL
                     EDALLLDGERILRALLNVADNARKAMSPGGVLTLRAYPQQDANVFVLEDTGEGMSPEV
                     LAHIFEPFYSSSKAGGTGLGMLIVKNVVEAHGGTVEVESEPGKGTRVRL"
BASE COUNT          727 a         1335 c         1254 g          707 t
ORIGIN      
        1 ccacgcaggc ctggacgatg gcctggagct gttccatgtt gttgaagtta taggcgggaa
       61 gggcgtagcc cccggacacc gccttcttga agagctccac ggtattcacc aggccgagct
      121 ccttgtaact cgtcatcgtt cgctccttgt gataggggga taggtgcccg gacagtctag
      181 gaagaggccc tcgggacgtc aagccccggg tcccgcggcc ccgggccgta cggattgtcc
      241 gggttgtccg ggcgccgggt acggtgtagg atgcggagca tggacggaaa ccgaggcccc
      301 cggtcggttc gatgccgcat ctccctcctt ctcgccttgt cgggcctcct ggcctcctgt
      361 tcccgtcccg taggactggt cctgatcgat cccgccctgc cggtcctgga ccccgagggg
      421 gcgaaggcct tctcgtcctt ccgcgtcccc ggcacgcgga gggcggtccg ggactaccct
      481 gtttcggaat ccgccgcctt cttcgggggg ctctcggctc cgcccccgtc cttcgtcctc
      541 ctcacgcccc tgttggcccc tgagtacccg gcggtccggg acgcctttcc ggaggccgtg
      601 atcctgggaa ccggcatccc cgcgggagaa cgctcccttt ccgcctcctg ggaccccgtc
      661 cccgcggccc gggaggcggg agtccgggcg gccgccttcc tgcggaaccg gggggagggg
      721 ctttccgagc ccccggaggc cgtgatcctg gtatccggcg ccgccggtgg cggtaccggg
      781 agcgggatgg cggcggaagc cttccgggaa gggcttctgt ccgcgcggcc ggataccccc
      841 ctgcggatcc tggagcttcc cggccaggcc ggggaggcgg acgtcctgat ccggggcctg
      901 gggggcccgg gggccaaggc ggccttcctg gatcccggga ccgggggatt ccgggccgcc
      961 cggctccttg cggaatccac ccccgaaggc gtccggggac cccggttcac ggttttccgg
     1021 tcggccctcc cccccccgcc ggggacgccg gcggacctcc tgctcctccg ggaaacccgg
     1081 gaccttttgc gggctttccg cgcggccctg aaggagggcc ggaccggcgc gatcgacgtc
     1141 ccggaagccc tccggatccc cgatcgggtg ccctgaaaag gcgtttcgct ttttttcaat
     1201 ttgtttgaca aaggaatggc cgatatctat aattcgtaca accgcaggta tttcactcat
     1261 ggggcaaaag gagccattcg aatgaaacga cgagtctccc gatcgatatg ccttgcgatc
     1321 ctactggtga gcctcctgtc cgcagggggc gtcttcgccc aggaaaagcc cgtcaacctg
     1381 gagtccaagg tcatcgacag cttcgacgac ccggaaggca atccctggtt cgtcatgggc
     1441 agcaagttct ccaccgcgga gttccccaag gtagcctccg tcaactcctg gcccatctcc
     1501 atctacggct acaaccccgc ggacaaggac aagctcaaga ccctgggcgt ggccatgctc
     1561 ttcgaccgga aggagtacaa ctgggtggac gtggtccccg gcaagaagac cggttccgga
     1621 gacagcgtga agtacgaacc ccaggaacgc aacctccccg gacgggtggc catgctggac
     1681 atgtggatct ggtccggcaa cttcaactac tacatcgagg ccttcatccg ggactacaag
     1741 ggcatcgtcc acaccatcta catgggggac ctcaaccacg tgggctggaa gaacttccgg
     1801 attaacatcc cctcgaacat cccccagtcc aagaagtacc ttcccaagct tgagggactc
     1861 aagcttgtga agttccggat ctggacccgg cccaccgagg tggtggcgat ccccgcgggg
     1921 gccgaggagc cggcccacaa gaaggccatc tacttctact tcgaccagct caaggtcctc
     1981 acggacacct acgaggacat gtatgacggc gacgcgctca cctcgcccga actcatcgag
     2041 cagacctgga gctcggggaa ataagggaga acagccatga agaagatcat cctaatcgcc
     2101 cttgccctgt gcctcgcggc cttcgcctcc gcccagaccg cggtcgggga cccgaacccc
     2161 aacgacatcg gagtggacac cgcacagcag aaactaaagg aagtctcggt ggacaagttc
     2221 gaggcagccg ggttctggaa agccttcatt tccccggacg agggagccat ccagtaccgc
     2281 ctcttcgaag gaagccccgc cggcaaggcc aacgagccca tcgcggacga acgctacgtc
     2341 gggatcgatc ccaaggtggc ggacaagttc gtcctgggcg tccgcacgga cttcttccgc
     2401 cgcggataca acgagatccg gatcctggcg gacaagccca tcccgatcga ggggatcagc
     2461 aagaccgtat ccgtctgggt ggccggccgc aaccttaacc acgtcctcaa gatcatgatc
     2521 ctggacgctt tcaacacccc cttcgagctc accgtgggca agctgaactt ccagggttgg
     2581 aagaagctga ccgtggcgat cccgccccag aacatggacg gcaagaccgg gatcatccag
     2641 cggaacttcc acttcatctc ccggatgggg atcaagatcg tgggattcaa gatcgagtgc
     2701 gatcccatgg aggccttcgg aacctattac ttctacatgg acgatctccg ggcggtgacg
     2761 gacctcttcg ccgaggatta ccgggatccg gacgacatga aggatacctg gtgatcgcgg
     2821 gattctgatc atccgagccc tcctttccgg gagggctttt ttttggaacc ggtatgaacg
     2881 actactacga gtttctccgc aggatcttct tcttcaagga cctctcggac caggagatac
     2941 tcctggtagc caacgcctgc cacgaggagc gccacacctc gggggacatc ctgttcctgg
     3001 agggcagcgc cgcggaccgc tgctacatcg tcctggaggg ccgggtggag gtctggaagg
     3061 actacaacga ggcaaaaccc agtcttttgg gagtgcacgg gccgggacac ttcttcgggg
     3121 agctggccct gctggacgaa cttccccgct cggccaccgt ggtggcccgg gaaaacgccc
     3181 gggtcctctt cctgtaccgg gaagacttcc ggaacctgat ccgcgaccac tcctccatcg
     3241 ccctgtccgt catggtctcc atctccttca tgctgcgctc ctccaacgag gtcttcctgg
     3301 aggacctcaa gaagcggaac accgagcttg agtccgcgta ccgggaactg gaggaggttc
     3361 acgcggaccg cctgcggaac gagcgcctct cgaccctggg caaattctcc tccatgatcc
     3421 tccacgacat ccggaacccc ctgtccatgt tgaagggact cctccagctc atgatcctga
     3481 acctccacga gccggagaag ctggaaggcc acgtccggaa cgccctggcc gagacggccc
     3541 gcctggaaaa cctggcctcg gagttcctgg actattcccg gggggagatc cgcctgaacc
     3601 tcggggtgac aaccgccgcc tggctgttca agcgggtgga ggaatccgtc cgggaacggt
     3661 tccggacctc ggggctgtcg gtggtgacgg actgccgcct ggaggacgcc ctgctcctgg
     3721 acggggaacg gatcctgcgg gccctgctga acgtggcgga caacgcccgg aaggccatgt
     3781 ccccgggcgg ggtcctgacc ctgcgggcct atccccagca ggatgccaac gtcttcgtcc
     3841 tggaggatac cggagagggc atgagcccgg aggtcctggc ccacatcttc gagcccttct
     3901 actcctccag caaggccggg ggcacgggcc tgggcatgtt gatcgtcaag aacgtggtgg
     3961 aagcccacgg gggcaccgtg gaggtggaat cggagccggg aaagggcacc cgggtccggc
     4021 tct
//
LOCUS       DAOTEI010000245         4004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_16611_length_4004_cov_5.706002, whole genome shotgun sequence.
ACCESSION   DAOTEI010000245 DAOTEI010000000
VERSION     DAOTEI010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16611_length_4004_cov_5.706002"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="P5313_15995"
     CDS             complement(<1..463)
                     /locus_tag="P5313_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY81918.1"
                     /translation="MEYRRLGSAGIKVSTLSLGSWVTYGKQVDVDAAAASLAAAYGAG
                     VNFFDNAESYAQGASESIMGAALRKLRWRRGSYLVSTKIYWGLAEGPNEKNTLNRKRL
                     LEGIDGSLERLSLDYVDLLFCHRPDPETTVEEVVWAMSDIVASGRAMYWGTS"
     gene            complement(465..1319)
                     /locus_tag="P5313_16000"
     CDS             complement(465..1319)
                     /locus_tag="P5313_16000"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRY81919.1"
                     /translation="MDKPAGMPTIAPEGASGRTRSLYDWVTEYIRRRNPKGRAAVVHR
                     LDRDSSGVVVFATGAAAKRELMSRWNQLALSRRYAALVEGAPDGESGVLDTWLRENRA
                     GTVYQAPPGTPGALRAVTRWRLLESGGGYSLLELELETGRKHQIRAQLSSAGLPVAGD
                     SRYGARTDPVGRLALHAVSIELERPFGDGVVEVESPVPESFLRPLRGREKGLYYVPGG
                     AKQRPSPAVGSPAAGSDTEGPGARAPGGGHMKEKSKQAKEPKKKAQKTLKEKRKEKKE
                     KAASKSEE"
     gene            complement(1493..1804)
                     /locus_tag="P5313_16005"
     CDS             complement(1493..1804)
                     /locus_tag="P5313_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81920.1"
                     /translation="MEPKPDDGVISESCGAPFGGAQMSFPVTFDLRIIYVLEGGGSIQ
                     SDLEALYTRLGVECSLLQGIFKPGAKYGRFGSRITVRNRAQFYAVYSEVGKLPYIKAV
                     I"
     gene            2046..2807
                     /gene="truA"
                     /locus_tag="P5313_16010"
     CDS             2046..2807
                     /gene="truA"
                     /locus_tag="P5313_16010"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRY81921.1"
                     /translation="MRNLLLILAYDGTAYKGWQRLPGSGRTVQEAVETALARVLGEDV
                     NIIGAGRTDAGVHAEGQAANVHTGSTRPPEDILADLNEALPRDIACRSLKEVPERFHA
                     RYWAIAKTYRYRLHAHPIPDPFSQRYSLHVPESLDLPAMEASAAELVGRRDFSAFANH
                     RGLKKGGERDLRTVRIQRNGQFVDILFTADGFLYNQARIMAGALIEAGLGRLGPKDIR
                     GLLETKDRSAAPGAAPAQGLCLVRVEFRTETPEEE"
     gene            2804..>4004
                     /locus_tag="P5313_16015"
     CDS             2804..>4004
                     /locus_tag="P5313_16015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY81922.1"
                     /translation="MTGPVRVLIVAASLGGSARHTPLGAARIVSALKACPASAGKIEP
                     ELLAVTVPGDPARLARTALDRRPRAVGFSLYVWNRDLLLAAARDLRRDRPDLVLFAGG
                     PEATADPAGVRAEGGLDFVVEGEGEESAPLALERALAGADPGEVPGVLGSGVPAPDGR
                     DREPVVSARAPGVPARVFGARAREPGVLARAELSRVPSPYLDGTLNVSEYGGGLWELA
                     RGCPFACHFCYESKGCRGVRSFPDEVVRAELRAFVRGGAREVFVLDPTFNADPARARD
                     ILTLLRREAPGIHFTFEIRSEFLDAAQARLFGSIPCSVQVGLQSARPEVLAVVGRPGF
                     DRKEFRRRTAMLSAAGVVFGLDLIYGLPGDDLAGFRESLDYALSLEPNHLDVFRLSVL
                     PGTVLADR"
BASE COUNT          540 a         1398 c         1377 g          689 t
ORIGIN      
        1 cgctggtgcc ccagtacatc gcacgcccgg aggcgacgat gtcgctcatg gcccagacga
       61 cctcctcgac ggtggtctcg gggtccgggc ggtggcagaa gaggagatcc acgtagtcca
      121 ggcttaggcg ctccagggag ccgtcgatgc cctccaggag gcgcttgcga ttcagggtgt
      181 tcttctcgtt cgggccctcc gccaggcccc agtagatttt ggtggagacg aggtagctcc
      241 cccgcctcca ccggagcttg cggagggcgg cgcccatgat ggattccgag gcgccctggg
      301 cgtaggattc cgcgttgtcg aagaagttga cccccgcccc gtaggccgcg gccagggagg
      361 ccgccgcggc gtccacgtcc acctgcttgc cgtaggtgac ccaggagccc agggagaggg
      421 tgctgacctt gatgcccgcg gaacccagcc gtcggtattc cattctattc ttcggacttg
      481 ctggcggctt tttccttctt ttccttccgc ttttccttga gggtcttctg ggcctttttc
      541 ttgggttcct ttgcctgctt gctcttttct ttcatgtgtc ctcctccggg ggcccgggct
      601 ccgggtccct ccgtatccga accggcggcc ggggatccga ccgcggggga gggccgctgc
      661 ttcgcccccc cgggtacata gtataggcct ttttcccggc cgcgcaaagg ccggaggaaa
      721 ctttcaggta cgggactttc gacctccacg acgccgtccc cgaagggacg ctccagctcg
      781 atggagaccg cgtgcagggc gagtcgtccg accgggtcgg tccgggcgcc gtaccgggag
      841 tccccggcca cgggcaggcc cgccgaggaa agctgggccc ggatctggtg cttccggccc
      901 gtctccagtt ccagttccag gagcgagtag ccgcctccgg attccagaag cctccaccgg
      961 gtgacggccc ggagggcgcc cggggttccc ggtggggcct ggtacaccgt gccggcacgg
     1021 ttctcccgga gccaggtatc caggacgccg gactcgccgt ccggagcgcc ctcgaccagg
     1081 gccgcgtagc gccgggacag ggcgagctgg ttccaccggg acatgagctc ccgtttggcc
     1141 gcggcgcccg tggcgaagac cacgaccccc gaggagtccc ggtccaggcg gtgcaccacc
     1201 gccgcgcgcc ccttggggtt gcggcggcgg atatactcgg tgacccagtc gtagaggctg
     1261 cgggttcggc cggaggcacc ctcgggggcg atggtcggca tacccgcggg cttgtccacg
     1321 atgaccaggt cctcgtcctc gtagagtatt tccggccggc cgggacggga cgcggtgccc
     1381 cgggctcgtc ccggggcggt tggccgcggc gtcttgcttg ttggtcgcgc ctcggcccct
     1441 gggcgcccgg gacgcggccg ggcgccgtcc ggattccggc tccgtcgcga ggtcaaatga
     1501 ccgcctttat gtaggggagc ttgccgacct cggaatagac cgcgtagaac tgggcccggt
     1561 tccggacggt gatgcggctg ccgaatcggc cgtacttggc cccgggcttg aagatcccct
     1621 ggagcaggga gcattccacc cccaaccggg tgtagagggc ttcgaggtcg gactggatcg
     1681 accctccccc ttccaggaca tagatgatcc ggaggtcgaa ggtgacggga aagctcatct
     1741 gggcaccccc gaagggggct ccgcaggatt cggagatgac gccgtcgtcg ggcttgggtt
     1801 ccatgagggt acgatgccat gagggaaggc gccatgtcga tagtccgttg cgattcgggt
     1861 atatgtgaac ttcgtataat tcgctccatc ttcgaattat gcgtgcttca aataatccgc
     1921 ctcgtcgccg aattatacgc gcttcgtata cttcgccccg tccccgaagt atacgcgcat
     1981 catataattc gcgccctcat ccgccccgct tccctaccgc ccccggaacg gatatcctta
     2041 tccttatgcg gaacctcctc ttgatcctgg cgtacgacgg aacggcctac aagggctggc
     2101 aaaggctgcc cggttcggga aggaccgtgc aggaggcggt ggagaccgcc ctggcccgcg
     2161 tgctggggga ggacgtcaac atcatcggcg cgggccggac ggacgcgggg gtccacgcgg
     2221 agggacaggc ggccaacgtc cacaccggtt ccacccgccc gcccgaggac atcctggcgg
     2281 acctcaacga ggccctcccc cgggacatcg cctgccgttc cctcaaggaa gtacccgaac
     2341 gattccatgc ccggtactgg gcgatcgcga agacctatcg gtaccgcctg catgcccacc
     2401 cgatcccgga tcctttttcc cagcgctaca gcctgcacgt cccggagtcc ctggacctcc
     2461 ccgccatgga ggcctcggcg gcggaactgg tcgggcggcg ggacttctcg gccttcgcca
     2521 accaccgggg cctcaagaag ggcggcgagc gggacctgcg gacggtccgc atccagcgga
     2581 acggacagtt cgtggacatc ctgttcaccg cggacgggtt cctgtacaac caggcccgca
     2641 tcatggccgg ggccctgatc gaggcggggc tgggccggct ggggcccaag gatatccgcg
     2701 gcctcctgga aacgaaggac cgttccgcgg ctccgggcgc ggcccccgcc caggggctct
     2761 gcctggtccg ggtcgagttc cggacggaaa cgccggaaga ggaatgaccg gtcccgtccg
     2821 ggtcctgatc gtcgccgcgt ccctgggcgg atcggcccgc cacacgcccc tgggggccgc
     2881 ccggatcgtc tcggccctga aggcgtgtcc cgcctccgcc gggaagatcg agcctgaact
     2941 gctcgccgtt acggtcccgg gcgaccccgc ccgtctcgcc cggaccgccc tggaccgccg
     3001 gccgcgtgcg gtcgggtttt ccctctacgt ctggaaccgg gatctcctgc tggccgcggc
     3061 ccgagacctg cggcgcgacc gtcccgacct ggtcctcttc gcgggcggcc ccgaggccac
     3121 cgcggatccc gccggggtcc gggcggaggg cggcttggat ttcgtcgtgg aaggggaggg
     3181 cgaggaatcc gcccccctcg ccctggagcg cgccctggcc ggggccgatc ccggggaagt
     3241 cccgggggtg ctgggttccg gggtcccggc gccggacggc cgcgaccggg aacccgtcgt
     3301 atccgcccgt gcacccggcg tacccgcccg ggtgttcggc gcccgcgccc gagagcccgg
     3361 cgtactcgcc cgggcggaac tctcccgcgt cccctccccc tacctggacg ggaccctgaa
     3421 tgtgtcggag tacggagggg ggctttggga gctcgcccgg ggctgcccct tcgcctgcca
     3481 tttctgctac gagtccaagg gctgccgggg agtccggagt ttccctgacg aggtcgtgcg
     3541 cgccgagctc cgggccttcg tccggggcgg ggcccgggag gtcttcgtcc tggacccgac
     3601 cttcaacgcc gatcccgccc gggcccggga tatcctgacc ctgctccgcc gggaggcccc
     3661 ggggatccac ttcaccttcg agatccgctc ggaattcctg gatgccgccc aggcgcgcct
     3721 ctttggatcc atcccctgtt cggtgcaggt cggtctgcag agcgcccggc cggaggtcct
     3781 ggccgtggtc ggccggccgg gcttcgaccg gaaggaattc cgccgccgca cggcgatgct
     3841 ctccgcggcg ggggtcgtgt tcgggctgga cctgatctac ggcctgcccg gggatgactt
     3901 ggcgggattc cgggagagcc tggactacgc cctgtccctg gagcccaacc atctggacgt
     3961 gttccggctc tcggtgctcc cgggcacggt cctggcggac cggg
//
LOCUS       DAOTEI010000246         3963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_16877_length_3963_cov_5.402508, whole genome shotgun sequence.
ACCESSION   DAOTEI010000246 DAOTEI010000000
VERSION     DAOTEI010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16877_length_3963_cov_5.402508"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="P5313_16020"
     CDS             <1..464
                     /locus_tag="P5313_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY81923.1"
                     /translation="SGIEAVLPLAEKEGVRLAIEPLHPMYAGDRSVINTMREALDLCE
                     GIGSPSLAVAVDVYHVWWDPELEAQIGRAGRLGALAAFHVCDWKTPLEDMLEDRGLMG
                     EGCIDIPRIRSWVERAGFRGFAEVEIFSRRFWAMDQDEYLGRILAAFREYV"
     gene            461..1612
                     /locus_tag="P5313_16025"
     CDS             461..1612
                     /locus_tag="P5313_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY81924.1"
                     /translation="MSERAIGIVMNGVTGRMGTNQHLVRSLLAVRAQGGLRARDGSVI
                     VPEPVLVGRDERKLKALAAEHGLRAWTTDLDAALSDKANQVYFDSQVTSLRPAAILKA
                     IAAGKAIYTEKPTATTLDEALGLCRAAKAAGIKNGVVQDKLWLPGLRKLKYLIDTGFF
                     GRILSVRGEFGYWVFDGFSQPAQRPSWNYRAEEGGGIILDMFCHWQYVISNLFGTIRS
                     VSALGATHLPKRVDEKGMPYDCTAEDSAYGTFELEGGVVCQFNSSWDVRVRRDDLFTM
                     QVDGTRGSAVAGLRECRIQGEAMTPKPVWNPDLDSPIDYFADWAKVPTRQTFDNAFKA
                     QWELFLLHLVQDDPFPWTLETGARGVQLAELALRSWKERRWIDIPDLGL"
     gene            1748..2191
                     /locus_tag="P5313_16030"
     CDS             1748..2191
                     /locus_tag="P5313_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81925.1"
                     /translation="MRRLKVLAAILIVAGFIVSCAGAPETPEPPPPEVKTVSGRPEAE
                     AAKQAADSARVKADEEKAAVAAKDEYTAAQEAYKKAETEFAAGRYTEAVAAYKLAEEG
                     FGKAQKLAADRKAAAQAAMKSADASLQATDERLKAIETEIEGGTP"
     gene            2188..2880
                     /locus_tag="P5313_16035"
     CDS             2188..2880
                     /locus_tag="P5313_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81926.1"
                     /translation="MRNLNRLVALAVLISLGTLAFAQHSLYDNPNYRNSQELRRQADL
                     AFQQGDYLKAEQLSKESERLSVIARQEAETQRLQWIANAWKNRASDRIGFGEKNDAAG
                     QLGPVWVEAKAAFALAVAEYDAKRYAESTEASKKVMDLMKDFAPAKAPAAAALPAFYT
                     VRLIPEARDCFWRIAEYPFIYGDPWKWKILYEANKDKIPQPDNPDLIEPGIVLTIPSI
                     AGELRSGTWNEK"
     gene            2945..3294
                     /gene="ssrA"
                     /locus_tag="P5313_16040"
     tmRNA           2945..3294
                     /gene="ssrA"
                     /locus_tag="P5313_16040"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3644..>3963
                     /locus_tag="P5313_16045"
     CDS             3644..>3963
                     /locus_tag="P5313_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HRY81927.1"
                     /translation="MASPLSNLIWSVADLLRGDYKQADYGKVILPFTLLRRLDCVLEP
                     TKAAVLAEAKAKKGEGELVLDRALCKASKQTFYNTSQHTLNTMLSDPDQLKQNLLHYL
                     SSFST"
BASE COUNT          721 a         1349 c         1298 g          595 t
ORIGIN      
        1 gctcggggat cgaggcggtc ctgcccctgg cggagaagga gggcgtccgt ctggcgatcg
       61 aacccctcca tcccatgtac gccggggacc ggtccgtcat caacacgatg agggaagccc
      121 tggacctgtg cgagggcatc gggagcccct ctctggccgt ggccgtggac gtgtaccacg
      181 tctggtggga ccccgagctg gaagcccaga tcgggcgggc cggccgtctg ggggccctgg
      241 cggccttcca cgtctgcgac tggaagaccc ccttggagga catgctggag gaccggggcc
      301 tcatgggcga gggctgtatc gatatccccc ggatccggtc ctgggtcgag cgggccgggt
      361 tccggggctt cgcggaagtg gagatctttt ccaggcggtt ctgggccatg gaccaggacg
      421 aatacctcgg ccgcatcctg gcggccttcc gggagtacgt atgagcgagc gagctatcgg
      481 gatcgtcatg aacggagtca ccggccgcat gggcaccaac cagcacctcg tccggtccct
      541 cctggccgtc cgggcccagg gcggcctgcg ggcccgggac ggatccgtga tcgtccccga
      601 gcccgtcctg gtcggcaggg acgagcgcaa gctgaaggcc ctggccgcgg agcacggcct
      661 ccgggcctgg acaacggacc tggacgcggc cctctcggac aaggccaacc aggtctactt
      721 cgactcccag gtcacctccc tgaggcccgc ggccatcctc aaggccatcg ccgcgggcaa
      781 ggcgatctat accgagaagc ccacggccac gaccctggac gaggcgctgg gcctctgccg
      841 ggcggccaaa gccgcgggga tcaagaacgg tgtggtccag gacaagctct ggctgccggg
      901 gcttcggaag ctcaagtacc tgatcgacac gggtttcttc ggccggatcc tgtccgtccg
      961 gggggagttc ggctactggg tcttcgacgg attctcccag cccgcccagc gcccctcctg
     1021 gaactaccgc gccgaggagg ggggcgggat catcctggac atgttctgcc actggcagta
     1081 cgtgatctcc aacctcttcg ggaccatccg ctccgtctcc gccctggggg ccacccacct
     1141 tcccaagcgg gtggacgaaa agggaatgcc ctacgactgc accgccgagg attccgccta
     1201 cgggaccttc gagctcgagg gcggggtcgt ctgccagttc aactcctcct gggacgtgcg
     1261 ggttcgcagg gacgacctct tcacgatgca ggtggacggc acccggggct ccgcggtggc
     1321 gggcctccgg gaatgccgga tccaggggga ggccatgacc cccaagcccg tctggaaccc
     1381 cgacctcgac tcccccatcg actacttcgc ggactgggcc aaggttccga cccggcagac
     1441 cttcgacaac gccttcaagg cccagtggga gctcttcctc ctgcacctgg tccaggatga
     1501 ccccttcccc tggaccctgg agaccggggc ccggggggtc cagctggcgg agctggccct
     1561 gcggagctgg aaggagcgcc gctggatcga catcccggac ctgggactct gaccggggag
     1621 gcggggccgg gaaccccgcc gttaccggtc gagacgggaa gatttttccc attcggcccc
     1681 ttcgggttga ctccacggag tttcgagccc acaatcaata ctatactctg ataatcagga
     1741 ggacatggtg agacgactca aggtcctcgc ggcgattcta atcgtcgcgg gttttatcgt
     1801 ttcctgtgcg ggggctccgg aaaccccgga accgcccccc ccggaggtca agaccgtaag
     1861 cggccgcccc gaggccgagg cggccaagca ggccgcggac tccgcccgcg tgaaggcgga
     1921 cgaggagaag gcggcggtgg cggccaagga cgagtatacg gccgcccagg aagcctacaa
     1981 gaaggcggag accgaattcg ccgcggggcg atataccgaa gccgtcgcgg cctacaagct
     2041 cgccgaggaa ggcttcggca aggcccagaa gctcgcggcg gacaggaagg ccgcggccca
     2101 ggccgccatg aagagcgccg acgcctcgct ccaggccacg gacgagcgac tcaaggccat
     2161 cgagaccgag atcgagggag gcacgccgtg aggaacctca acagactcgt cgccctggcc
     2221 gtgctgatct ccctcgggac cctcgccttc gcccagcaca gcctctatga caacccgaac
     2281 tacaggaatt cccaggagct ccgccggcag gcggacctcg ccttccagca gggggactac
     2341 ctcaaggccg aacagctctc caaggagtcc gaacgcctct cggtgatcgc gcgccaggag
     2401 gcggagaccc agcgccttca gtggatcgcc aacgcctgga agaaccgcgc ctccgaccgg
     2461 atcggcttcg gcgagaagaa cgacgccgcg ggccagttgg gtcccgtatg ggtcgaggcg
     2521 aaggccgcct tcgccctggc cgtcgcggaa tacgacgcca agcgctacgc cgagtccacg
     2581 gaagccagca agaaggtgat ggacctgatg aaggacttcg cccccgccaa ggccccggcg
     2641 gccgccgcac taccggcctt ctatacggtt cggctgatcc cggaggcccg ggactgtttc
     2701 tggcggatcg cggagtatcc cttcatctac ggggatccat ggaagtggaa gatcctctac
     2761 gaggcgaaca aggacaagat cccccagccg gacaatcccg acctgatcga gcccgggatc
     2821 gtcctgacca tccccagcat cgccggggag ctccgatcgg gcacctggaa cgagaagtag
     2881 ggaccgtacg ccccgccgct tgaccgctgg ccggcggcgg ggtatatttt ttcttgaccc
     2941 ctacgggggt gccccggttt cgactggtgc gattggggca tgggccgcag gtggagcgcc
     3001 gacctcctaa cattcggcaa ccctttaact gccaacaacg acagttacgc tctcgccgcg
     3061 taagcggcgg aacgcgggaa ccggctgcgc cgcctcgagc ggccgtcatt cccgacacga
     3121 tcgaggctgg tcgactttcg ggcccgaacg aaagtaggac gagaataacc gggatacggg
     3181 cccgccgcgc gccgcgcagc caagcgggtc ccgacaatcc aatgcgcggc taaacctgta
     3241 gaggctcctg tcacgcgcat caggacgggg gttcgactcc ccccacctcc agtaacggcc
     3301 gccgctccac caggaacggc ggccgtacca ttttaagtgg gggagtcgaa cgggcgttcg
     3361 cgacggcgta gctccgagcc ggcgaggagc ggagccggcc gcgcccaagg acgggcgcgg
     3421 agcgaacgcc ccggcccgca gggaggcgcg atgatcgcgc cgaccgaagg gtcgactccc
     3481 gccacctcca gtaaccgccg cagttccagc atggaaggcg gcggttttgt ttcaatgggg
     3541 gagtcgaacc gagccgacga tcccgaggcg gggcgaatct cgttgccatc ccccccgcca
     3601 agggcgtaca atagcctcca aaatagaatt catggagttc tagatggcgt ctccgctttc
     3661 caacctgatc tggtccgtcg cggatctcct tcgcggggat tataaacagg ccgactacgg
     3721 caaggtgatc cttcccttca ccctcctgcg ccgcctggac tgcgttcttg agcccaccaa
     3781 ggcggcggtg ctcgccgagg ccaaggcgaa gaagggcgag ggcgagctcg tcctcgaccg
     3841 ggcgctctgc aaggcctcca agcagacctt ctacaacacc tcccagcaca ccctgaacac
     3901 gatgctctcg gacccggacc agctcaagca gaacctcctc cactacctgt cgagcttctc
     3961 gac
//
LOCUS       DAOTEI010000247         3865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_17462_length_3865_cov_4.754068, whole genome shotgun sequence.
ACCESSION   DAOTEI010000247 DAOTEI010000000
VERSION     DAOTEI010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17462_length_3865_cov_4.754068"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="P5313_16050"
     CDS             complement(<1..325)
                     /locus_tag="P5313_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81928.1"
                     /translation="MRWEISKLVRGRDAYFTVRWSPFVRAEKYAIHTGVPAMGGVAEL
                     YWKDKGGKLNQFCVARSWYGGLRSLMRERIDPDLEKDPVRLAVLRDHKDEIYFRYSLS
                     ESNSDM"
     gene            504..2114
                     /locus_tag="P5313_16055"
     CDS             504..2114
                     /locus_tag="P5313_16055"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRY81929.1"
                     /translation="MKKLVFVTGGVCSSLGKGVTASSIGSLLEARGLTVRMMKIDPYL
                     NVDAGTMNPYQHGEVYVTDDGAETDLDLGNYARFTNAPIARANSITTGQVYQEVIEKE
                     RQGRFLGRTVQVIPHITDAIKARIHAVADNPDVDVTIVEVGGTVGDMESIPFLEAARQ
                     IIHEEGHQNAVSVHVTLIPRVSGGELKTKPTQHSVKELQEVGIQPDVLVVRCDEPVEE
                     GLRRKIAAFTNVDFEGVISAHDVPVTIYEIPLVFHDQKLDTYLLRRMNIESRHADLRA
                     WKEMVRKFREPRRRVRIGVVGKYMELVDAYKSVWEALHHGGIANDAVVDIVRIDSGDF
                     EGEQADPAKALGDVRGILIPGGFGHRGTSGMVAAVRWAREHKMPCFGICLGMQIMTIE
                     WARTVLGWEDADSSEFQPDSKHPVVSLLEEQLEVKNYGGTMRLGASVSVLKEGTRVRE
                     AYGSETIQERHRHRYEFSNAYRADMEASGLRITGLTPDGALVECVEWPDHPWGVGVQF
                     HPEFKSRPVAPHPLFRAFVKACLGYAGQ"
     gene            2101..2739
                     /gene="lptC"
                     /locus_tag="P5313_16060"
     CDS             2101..2739
                     /gene="lptC"
                     /locus_tag="P5313_16060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRY81930.1"
                     /translation="MRGSRTAPGGPRPGSAARRPGSSTAPVAAPPAAALCAVLAAAFL
                     VSACSFDYGQALAEDLAERTPNAVFTGFTHTVVQDNRPILRIEAARAEIFDRKSLVRL
                     SDVSFTEMRDGRVLAYGSADGASLRTDTEDAEFFGSVSLRSEEEGVSIRTAYLSWNAS
                     DRVLSGPEDGETGIERDDGSFLRGSGFRADARRKGISFSGKTEGLLVREAGE"
     gene            2736..3425
                     /locus_tag="P5313_16065"
     CDS             2736..3425
                     /locus_tag="P5313_16065"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81931.1"
                     /translation="MRGSVGRAVLARIPPRILALALLACSVPGFGAETFRFSADRMES
                     VIAEGRERALLSGNARLAADDLTITADVIELSGKDWRWARCSGRVSASDETQGIRLTT
                     EVLVYDRQTRISRLEGDSVLEDSRNRVVLKAYWMEHDDERKTVTARVGVRVFKDSTSA
                     RAASLTYRRDEQSLELSGSARVLRDGDEYRAERIVMDLETDEIVLSGGVSGTVIKKKK
                     PEEANPGETSP"
     gene            3422..>3865
                     /locus_tag="P5313_16070"
     CDS             3422..>3865
                     /locus_tag="P5313_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY81932.1"
                     /translation="MSAPAESHVLEARGIVKRFGRKTVIRGVGFSMRSGEVVGLLGPN
                     GAGKTTVFYTILGFLKPDQGAVILDDRDITRLPMYRRARLGVSYLPQEPSIFRRMTVE
                     KNVWAILETRDDLSLEEKKERLEALLSEFGIQDIRRSSLLDSFGDK"
BASE COUNT          626 a         1322 c         1339 g          578 t
ORIGIN      
        1 ccatgtcgga gttggactcg gacagggagt agcggaagta gatctcgtcc ttgtggtccc
       61 ggaggacggc caggcgcacg gggtcctttt ccaggtccgg gtcgatccgc tccctcatca
      121 gggaccgcag gcccccgtac caggaccggg ctacgcagaa ctggttgagc ttgccgccct
      181 tgtccttcca gtagagctcc gcgaccccgc ccatggcggg caccccggtg tggatggcgt
      241 acttttccgc ccggacgaag ggcgaccagc gcactgtgaa gtaggcgtcc cgtccccgga
      301 cgagcttgga aatttcccac cgcatcagcc tgaacatcct ccgattcctg aagccggttc
      361 cggggcgaag tcccggaacc gaaccgcggg ccggccgcgc aggcccgcgc cccatgcggg
      421 gcatggtaca ccaagccggc ggtccgtgta cacgagccgg caccgcttct cactccctcc
      481 cccgccgggt ataatggaat tccatgaaga agcttgtatt cgtgacgggc ggcgtgtgct
      541 ccagcctcgg caagggggtc accgcctcgt ccatcggctc cctcctggag gcgcgcgggc
      601 tcaccgtgcg catgatgaag atcgacccct acctgaacgt ggacgcgggg acgatgaatc
      661 cctaccagca cggcgaggtc tacgtgaccg acgacggggc ggagacggac ctggacctgg
      721 gcaactacgc ccggttcacg aacgccccca tcgcccgggc caacagcatc accaccggcc
      781 aggtctacca ggaggtgatc gagaaggagc gccaggggcg cttcctgggc cgcacggtgc
      841 aggtgatccc ccacatcacg gacgccatca aggcccggat ccacgcggtg gcggacaacc
      901 ccgacgtgga cgtcaccatt gtggaggtgg gcggcaccgt gggcgacatg gagtccatcc
      961 ccttcctgga ggccgcccgg cagatcatcc acgaggaggg gcaccagaac gcggtctccg
     1021 tccacgtgac cctgatcccc cgggtctccg gcggggagct caagaccaag cccacccagc
     1081 actcggtcaa ggagctccag gaggtgggga tccagccgga cgtcctagtg gtccgctgcg
     1141 acgagccggt ggaggaaggg ctgcgccgca agatcgcggc cttcacgaac gtggacttcg
     1201 agggggtgat ctccgcccac gacgttcccg tgacgatcta cgagatcccc ctggtcttcc
     1261 acgaccagaa gctggatacg tatcttctcc ggcgcatgaa catcgagagc cggcacgcgg
     1321 acctgcgggc ctggaaggag atggtgcgca agttccggga gccccggcgc cgggtccgca
     1381 tcggggtggt gggcaagtac atggagctgg tggacgccta caagagcgtc tgggaggccc
     1441 tgcaccacgg cgggatcgcc aacgacgcgg tggtggacat cgtccggatc gattccgggg
     1501 atttcgaggg cgagcaggcg gatcccgcca aggccctggg ggacgtccgg ggcatcctga
     1561 tcccgggcgg cttcgggcac cggggcactt cgggcatggt ggccgcggtc cgctgggccc
     1621 gggagcacaa gatgccctgc ttcggcatct gcctgggcat gcagatcatg accatcgagt
     1681 gggcccgcac ggtcctgggc tgggaggacg cggactcctc ggagttccag ccggactcga
     1741 agcaccccgt cgtgagcctc ctggaggaac agctggaggt caagaactac ggaggcacga
     1801 tgcgcctggg agccagcgtc tcggtcctga aggagggcac ccgggtccgg gaggcctacg
     1861 ggtccgagac catccaggag cgccaccgcc accgctacga gttctccaac gcctaccggg
     1921 cggacatgga ggcttcgggc ctccggatca cggggctgac cccggacggt gccctggtgg
     1981 agtgcgtgga gtggccggac cacccctggg gcgtgggcgt ccagttccac cccgagttca
     2041 agtcccggcc cgtggccccc caccccctgt tccgggcctt cgtcaaggcg tgcctggggt
     2101 atgcggggca gtaggacggc tcccgggggg ccgcgtcccg gatccgcggc gcgccgcccc
     2161 ggatcctcca cggccccggt cgcagcccct ccggccgccg cgctctgcgc agtcctggcc
     2221 gcggccttcc tcgtctcggc ctgcagcttc gactacggcc aggccctggc ggaggacctg
     2281 gcggagcgca cccccaacgc cgtattcacg ggcttcaccc acaccgtggt ccaggacaac
     2341 cggcccatcc tcaggatcga ggccgcccgg gcggagatct tcgaccggaa aagcctggtg
     2401 cggctctcgg acgtctcctt cacggagatg cgggacggcc gggtcctggc ctacggctcc
     2461 gcggacgggg cctcgctccg gacggacacg gaggatgccg agtttttcgg ttccgtgagc
     2521 ctccgctccg aggaggaggg cgtctccatc cgcacggcct acctttcctg gaacgcctcg
     2581 gacagggtcc tctcgggccc ggaggacggg gagaccggga tcgaacggga cgacggctcc
     2641 ttcctccggg gctcgggctt ccgggccgac gcccggcgca agggcatctc cttttccgga
     2701 aaaaccgaag gtctcctggt ccgggaggcc ggggaatgag gggctccgtc ggccgcgccg
     2761 tcctcgcccg gatcccgccg cggatcctcg ccctggccct ccttgcgtgc tcggtcccgg
     2821 gcttcggggc ggagaccttc cggttttccg cggaccgcat ggagagcgtc atcgccgagg
     2881 gccgggagcg cgccctcctc tcgggcaacg cccgactggc cgccgacgac ctcacgatca
     2941 ccgcggacgt catcgagctg tccggcaagg attggcgctg ggcccgctgt tccggcaggg
     3001 tgagcgcctc cgacgagacc cagggcatcc ggctgaccac ggaggtcctg gtctacgacc
     3061 gccagacccg gatcagccgc ctggaggggg actccgtcct ggaggactcc aggaaccggg
     3121 tggtcctcaa ggcctactgg atggaacacg acgacgaacg gaagacggtc accgcccggg
     3181 tgggggtgcg ggtcttcaag gactcgacct ccgcccgcgc ggcctccctg acctaccgcc
     3241 gggacgagca gagcctggag ctttcgggtt ccgcacgggt cctccgggac ggggacgagt
     3301 accgggccga gcgcatcgtg atggacctgg agaccgacga gatcgtcctg tccggagggg
     3361 tctcgggcac ggtgatcaag aagaagaagc ccgaggaggc gaaccccggg gagacctccc
     3421 cgtgagcgct cccgcggaga gccacgtcct ggaggcccgg ggcatcgtca agcgcttcgg
     3481 ccgcaagacc gtcatccggg gggtgggttt ttccatgcgc tccggcgagg tggtggggct
     3541 cctgggtccc aacggggccg ggaagaccac ggtgttctac accatcctgg gtttcctcaa
     3601 gcccgaccag ggggccgtga tcctggacga ccgggacatc acccgcctgc ccatgtaccg
     3661 gagggcccgg ctgggggtct cctacctccc ccaggagcct tccatcttcc ggcgcatgac
     3721 cgtggagaag aacgtatggg ccatcctgga gacccgggac gacctgagcc tcgaggagaa
     3781 gaaggagcgt ctggaggccc tgctctcgga gttcggcatc caagacatcc ggagaagctc
     3841 cctgcttgac agcttcggcg ataag
//
LOCUS       DAOTEI010000248         3851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_17558_length_3851_cov_4.445996, whole genome shotgun sequence.
ACCESSION   DAOTEI010000248 DAOTEI010000000
VERSION     DAOTEI010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17558_length_3851_cov_4.445996"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..918)
                     /locus_tag="P5313_16075"
     CDS             complement(109..918)
                     /locus_tag="P5313_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HRY81933.1"
                     /translation="MGREPSARGKEAFLQRYERIFGDRFPGLKAALEGPPDSVEYAEG
                     LARPYRMDRASVLAARSLPLPEEGRVLDACAAPGGKSLVLASRMADRPGVTLTSNELS
                     ADRRRRLRDVLAIHLPPELAGRVAVSGFDAASRARREREAWDAILLDAPCSSERHVLA
                     SPAHLAAWSEARVRNLAARQWSLLSAAFLLLKPGGALVYSTCALSPEENDGVAGRLGA
                     KYGDRVSYEPPSAEDAGPEAPVFEGTEFGILVLPDAARGAGPMYVCRVRKD"
     gene            1014..1670
                     /locus_tag="P5313_16080"
     CDS             1014..1670
                     /locus_tag="P5313_16080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY81934.1"
                     /translation="MPPALIFDLDGTLVDTLPDIAAAMNAALETRGFPTLPRDAYRSR
                     VGWGSRELSRLCLPEARGTDEEIRFLDREFRRRYEAAPAVRSVPFPEIPAVLAELQDR
                     GIHLSILTNKPEEAVQPLLAALFPDIRFFRTLGAADGRPRKPDPAAALGIARDLGVPP
                     EGVYFVGDSAVDMRTARNAGMVPIGVAWGYRDVPELEAEGAARILRSPRDLLAILSEP
                     "
     gene            1748..3508
                     /locus_tag="P5313_16085"
     CDS             1748..3508
                     /locus_tag="P5313_16085"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRY81935.1"
                     /translation="MDRKELQDRARAYIDQETEVAFRREIEDLLSREDWKELEDRFYR
                     DLEFGTGGLRGKIGGGTNRMNLLVVRKATQGLCDYLKSALPGKKLSACIAYDSRRYSA
                     AFAEATALVFAANGIKAWLFSALRPTPELSFAIRLLGADTGVVVTASHNPPAYNGYKA
                     YWGDGAQVIAPHDEGIIARVNSVGTVRTMGRAEALAAGLLAVIDKEVDEPYVAMVKSR
                     LFRPALIREMAGSVKIVYSPLHGTGALHFERVLGDLGLKVVTVPEQRDPDGEFPTVAF
                     PNPEEAAALKMALDLGKSVKADVVMATDPDADRLGIAVPDGAGGFALITGNQLGALLA
                     DYILLTRKELGTLPRKPATIKSIVTTELQARIAEAYGATCFDCLTGFKWIADLMRRFE
                     TDGRGYEFVYGTEESYGYLVENEVRDKDGISAAAVTAEMTLYWRGKGKSLLQRLDDLY
                     RTHGFYEEKGISKYFEGASGMALMGGIMEAYRKAQPSAFGGVPVARIRDLKAGKVWEP
                     ARPGSEAPAGLPASDVLQWWLADGTLVTVRPSGTEPKIKYYILARTEVPGGSLEAARA
                     ESARKVKAIEADIRAVVDSK"
     gene            3505..>3851
                     /locus_tag="P5313_16090"
     CDS             3505..>3851
                     /locus_tag="P5313_16090"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY81936.1"
                     /translation="MNRYGYLRRIRARGSRSGTAPASSRGRLDLARLRELWGRASFAV
                     LDFETTGLDSRADRICEIGALRLSETGVEAASLDLLVNPGLPIRPEASRVSGITDEMV
                     RNARPVEDALPELL"
BASE COUNT          617 a         1375 c         1315 g          544 t
ORIGIN      
        1 atcaccagcg cgtgttcacc aatctgcctg ccaaaggcct gattaccgac atcgtctccg
       61 aagccccgcc ggatcccggg cctgaacggc ccgcgcttac cgaagccttc agtccttgcg
      121 gaccctgcag acgtacatgg gtcccgcccc ccgggcggcg tcgggtagga ccaggattcc
      181 gaactcggtt ccctcgaaca cgggagcctc cggtcccgca tcctcggccg acggcggctc
      241 gtacgagact cggtccccgt acttggcgcc caggcggccc gccaccccgt cgttctcctc
      301 cggggacagg gcgcatgtgg aatagaccag ggctcccccg ggcttgagca ggaggaaggc
      361 cgcggagagc agggaccact gccgggcggc caggttccgc acccgggcct ccgaccaggc
      421 cgccaagtgg gcgggggaag ccaggacgtg ccgctcgctg gagcagggtg cgtccaggag
      481 gatcgcgtcc caggcttccc gctccctccg ggcgcgggag gccgcgtcga agcccgatac
      541 ggccaccctt cccgcgagct ccgggggcag gtgaatggcc aaaacgtccc gcagacgcct
      601 cctccggtcc gcggaaagct cgttggaggt cagggtcacc cccggccggt ccgccatccg
      661 ggaggccagg accagggact ttccgcccgg ggccgcgcag gcgtccagga cccggccctc
      721 ctccgggagg ggcagggacc gcgcggccag gacggaggcc cggtccatgc ggtagggccg
      781 ggccaggcct tccgcgtact ccacgctgtc cggcggccct tccaaggcgg ctttcaggcc
      841 cgggaaccgg tccccgaata tgcgttcgta cctctgcagg aaggcttcct tcccccgtgc
      901 ggacggttcc cggcccatgc ggaattgaca ctcccttccg gtgcggctag actcggcggc
      961 gccgcggata ccccataccc tacaccggcc ggcggcggag cggatagaac cccatgcccc
     1021 cagccctcat cttcgacctg gacggcactc tcgtcgacac ccttccggac atcgcggcgg
     1081 ccatgaacgc cgccctggag acacggggct tccccaccct gccccgggac gcctaccgct
     1141 cccgggtggg ctggggatcc cgggagctct cccggctctg cctgcccgag gcccgcggga
     1201 ccgacgagga gatccgcttc ctggaccggg aattccggcg gcgctacgag gcggcccccg
     1261 cggtccgatc cgtccccttc ccggagatcc ccgcggtcct ggcggagctc caagaccggg
     1321 ggatacacct ttccatactc acgaacaaac ccgaggaggc cgtacagccc ctcttggccg
     1381 ccctcttccc ggacatccgg ttcttccgga ccctgggcgc cgcggacgga cgcccccgca
     1441 agcccgatcc cgctgcggcc ctggggatag cccgggacct cggcgtgccg cccgaagggg
     1501 tctacttcgt cggggactcc gccgtggaca tgaggacggc ccggaacgcc gggatggtgc
     1561 ccatcggcgt cgcctggggc tatcgggacg tcccggagct cgaggcggag ggggccgctc
     1621 ggatactgcg gagcccccgg gacctcctgg ccatcctgtc cgaaccttga ttatactgga
     1681 agcatagcgt cggccggtcg gccttccgac ccgccgtgcg acatgtacca accgccggga
     1741 ggaagccatg gacaggaagg aactgcagga ccgggcccga gcctatatag accaggaaac
     1801 cgaggtcgcc ttccggagag agatcgagga cctcctttca agggaggact ggaaggagct
     1861 ggaggaccgg ttctaccggg acctcgagtt cgggaccggg ggcctccggg gcaagatcgg
     1921 cgggggcacc aaccgcatga accttttggt ggtacggaag gccacccagg ggctctgcga
     1981 ctacctcaag tccgccctgc cggggaaaaa gctctccgcc tgcatcgcct acgattcccg
     2041 gcgctactcc gcggccttcg cggaggcgac ggccctggtc ttcgcggcca acgggatcaa
     2101 ggcctggctc ttcagcgccc tccggcccac cccggaactc tccttcgcga tccggctcct
     2161 gggggcggac acgggcgtgg tggtcaccgc cagccacaat cccccggcgt acaacggcta
     2221 caaggcctac tggggggacg gggcccaggt gatcgccccc cacgacgagg ggatcatagc
     2281 ccgggtgaat tccgtgggaa ccgtccgcac gatgggccgc gccgaggccc tggccgccgg
     2341 actgctggcg gtgatcgaca aggaagtgga cgagccctac gtcgccatgg tcaaatcccg
     2401 actcttccgg cccgcgctga tccgggagat ggccgggagc gtgaagatcg tctactcccc
     2461 cctgcacggc acgggggccc tgcacttcga gcgcgtcctg ggggacctgg gcctcaaggt
     2521 cgtgacggtc ccggagcaga gggatccgga cggggagttc cccaccgtgg ccttccccaa
     2581 ccccgaggag gccgcggccc tcaagatggc cctggacctg gggaagtccg tgaaggccga
     2641 cgtggtcatg gccacggacc ccgacgcgga ccgcctgggc atcgcggtgc ccgacggggc
     2701 gggcggattc gccctgatca cgggcaacca gctgggcgcc ctcctggcgg actacatcct
     2761 tcttacccgg aaggagctcg gcaccctgcc ccgcaagccc gccaccatca agagcatcgt
     2821 gaccacggag ctccaggccc ggatcgccga ggcctacggg gcgacctgct tcgactgcct
     2881 tacgggcttc aagtggatcg ccgacctcat gcggaggttc gagaccgacg gccgaggata
     2941 tgaattcgtg tacggcaccg aggagagcta cggctacctg gtggagaacg aggtgcggga
     3001 caaggacggg atctccgcgg cggccgtgac cgcggagatg accctgtact ggcgcggcaa
     3061 ggggaagagc ctccttcagc gcctggacga cctctaccgg acccacggct tctacgagga
     3121 aaagggcatc agcaagtatt tcgagggagc ctcaggcatg gccctcatgg ggggcatcat
     3181 ggaggcctac cgtaaggccc agccctcggc cttcggaggg gttcccgtgg ctcggatccg
     3241 ggacctcaag gccggcaagg tctgggagcc cgcccggccc ggatcggaag cccccgcggg
     3301 gctcccggcc agcgacgtcc tccagtggtg gctggcggac gggaccctgg tcaccgttcg
     3361 gcccagcggt acggagccca agatcaagta ctacatcctg gcccgcaccg aggttcccgg
     3421 agggtccctg gaagccgccc gggcggagag cgcccggaag gtcaaggcca tcgaggccga
     3481 catccgggcg gtcgtggatt ctaagtgaac cggtacggat acctccgaag gatccgggcc
     3541 cggggaagtc gatcggggac cgcccccgcg tcctcccgcg gccgcctgga ccttgcccgg
     3601 cttcgggagc tctggggccg ggcgtccttc gcggttttgg acttcgagac caccgggctg
     3661 gacagccgcg cggaccggat ctgcgagatc ggggccctcc ggctctcgga gacgggagtc
     3721 gaggccgcta gcctggacct cctggtgaac cccggcttgc ccatccggcc ggaggcctcc
     3781 cgggtcagcg gcatcaccga cgagatggtg aggaacgccc ggcccgtgga agacgctctc
     3841 ccggaactgc t
//
LOCUS       DAOTEI010000249         3749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_18288_length_3749_cov_4.104764, whole genome shotgun sequence.
ACCESSION   DAOTEI010000249 DAOTEI010000000
VERSION     DAOTEI010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18288_length_3749_cov_4.104764"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="P5313_16095"
     CDS             complement(<1..1303)
                     /locus_tag="P5313_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81937.1"
                     /translation="MATFERHEGRNLKGIDPDAKDRDLEQYGIWVKAEPQDVLEEPDI
                     EDASLLSEAPEEAAVLDESFLTDEEEVFLGAGDRPAGRPRAEDADVFLSTSETPSLHE
                     EELSIPSLDESPAELPEDLPRIPDMAEFESLEDLGVPIEEFTPDETRKPSPRGPELEE
                     IQDVSDFGLEEAEPVLEAGTETDFESIDIDLKFDDTLPPSPAAQGAKIDRPQPSGADG
                     FETVTDFDDFLSGSDAGPAETAEPLSPPARASFEHKHPAESDDFSLEDIPEIPGGQPV
                     DDLAELERDLATGPGRPSVRAQAAEDSSSDILLKIAGELSSIKEELVSLKSQIAAMKS
                     ASPAPAEGIEEAAKAESGKAGGFFDEEEDETIALTGDELDNILNTAEFSEESAEPAPL
                     QEVPRRSKPWKTCFRRRGTTLPNRPPSTPILPASRSSASPTI"
     gene            1493..1984
                     /locus_tag="P5313_16100"
     CDS             1493..1984
                     /locus_tag="P5313_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81938.1"
                     /translation="MGIRLAVSAYIGLAVYCLLSLLLGPMGMAAYDDLETRVRGMREN
                     LARLESLNSEARARKDSALSDPEALALEARTLGYVGPGQVVVRLGFPEASPDPGDLGT
                     LVPFDPPRGMEDAELKAASLAAALVAAFAGFLIRSDRRGSRQRRGPEGRVSPAGTASA
                     RGL"
     gene            complement(1947..2336)
                     /locus_tag="P5313_16105"
     CDS             complement(1947..2336)
                     /locus_tag="P5313_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRY81939.1"
                     /translation="MQVPIDRIKVRRRVRKEPGDIDALAASMNVHGLLHPIVLDRGNR
                     LIAGGRRLAAAKRLGWKTINALIVDADDKADRLELELEENVQRSALSYLELEEGYARL
                     ERMRRPNIFRRIWEALKALVRRLFRRD"
     gene            complement(2388..>3749)
                     /locus_tag="P5313_16110"
     CDS             complement(2388..>3749)
                     /locus_tag="P5313_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRY81940.1"
                     /translation="DQVRLQMEKATVEGALITIENETGHVKAVVGGSKYDQSNQLIRA
                     TQSLLMPGSAFKPLYYSAAIDTRKFTEGSLIYDTPVVFYNDDGTPYIPLNYRGEWKGP
                     VLTWLALARSMNVPSVKVLDAIGFDAAINRAAALLDVTDPAEIRRTFPRLYPLALGVI
                     GVSPLKMARAYSIFANHGREVTPIAIRSVEDRQGRIFLEPEKELRTQQKKKGSAIQVI
                     SPLNAYVMTDMLRRTVTDGTLNYPTAGGKLFTYKDEEGKKYTIPSAGKTGTTQNWADA
                     WTVGFTPYMTTAVWFGFDRPGNSLGVTQSGAAIAGVLWANYMEAIHRGLPFKDFIRPQ
                     AGLVDVKVCAKSGLLPTDYCDEGLVGLLYYEGTQPHQYCDLHQFSSQAADRGMQTLSR
                     QGSVFGDVDIDTTLTIDLPDLEQLLRNPAPQTPSAPAPGTQPAPGTPPPPPAEYTTPP
                     VLE"
BASE COUNT          586 a         1150 c         1267 g          746 t
ORIGIN      
        1 agatcgtcgg agaggcggag ctcctcgatg ccggaagaat cggagtcgac ggaggccggt
       61 ttggaagcgt agtcccccgt ctccggaagc aggtcttcca gggcttcgac cgtctgggaa
      121 cctcctggag aggggctggc tccgcgcttt cctcggagaa ttcggcggta ttcaggatat
      181 tgtccaactc gtccccggtc agggctatcg tctcgtcctc ttcctcgtcg aagaatccac
      241 cggccttgcc cgactcggct ttcgccgctt cctcgatccc ctcagccgga gccggggagg
      301 ccgatttcat ggcggcgatt tgggacttga gggaaacgag ctcttccttg atcgaggaga
      361 gttcgcccgc gatcttgagg agaatgtccg aggacgagtc ttcggcggcc tgcgcgcgca
      421 cggacgggcg acccgggccg gtcgcgagat cccgttcgag ctccgccaag tcgtctacgg
      481 gctgtccgcc cggaatttcc gggatgtcct ccaggctgaa atcgtccgat tccgcgggat
      541 gcttgtgttc gaaggaggca cgcgcggggg gcgacaaggg ttccgcggtt tccgcggggc
      601 cggcatccga cccggaaagg aagtcgtcga aatccgtgac cgtctcgaac ccgtcggcgc
      661 ccgaaggctg ggggcggtct atcttggccc cctgggcggc ggggctcggc ggcagggtgt
      721 cgtcgaattt gaggtcgata tcgatggatt cgaaatccgt ttccgtaccc gcttccagca
      781 cgggctcggc ctcttccaga ccgaaatcgg agacatcctg gatttcctcg agttcggggc
      841 cccgagggga aggtttccgg gtttcgtcgg gggtgaactc ctcaatcggc acccctagat
      901 cctccaggga ctcgaattcg gccatatcgg ggatccgggg aagatcctcc gggagctccg
      961 cgggcgattc atccagggag gggatggaga gctcctcctc gtgcagggac ggagtctccg
     1021 aggtcgacag gaagacatcc gcgtcctccg cccggggccg tccggccggt cggtcgccgg
     1081 cacccaggaa gacctcctcc tcatccgtca ggaaggattc atccaggacc gcggcctcct
     1141 ccggagcctc ggaaagcagg gaagcgtcct cgatgtccgg ttcttccagg acatcctgcg
     1201 gctccgcctt aacccatatt ccgtactgct cgaggtcccg atccttggcg tcggggtcga
     1261 ttcccttaag gttccgtcct tcgtgccgct caaaggtcgc catggtcgct ccgttttacg
     1321 agggtcgttc tgatactgta tcggcaggga tcatctccga ataaacgacc gtttttaagt
     1381 ctaatcgggg cctatctcgt tgtcaatgta ggacgatacc gcgtttgcgg gattcttcgg
     1441 cgcaggcggt aaacacgtaa agacggggcc cgttccgtcc gtaaatgaat cgatgggtat
     1501 tcgactggcc gtcagcgcct acatcggact tgccgtgtac tgcctcctgt ccctcctgct
     1561 cgggcccatg gggatggccg cctatgacga cctggaaacc cgggtccggg ggatgcggga
     1621 gaacctcgcg cggttggaat ccttgaattc cgaggcccgg gcccgcaaag attccgccct
     1681 gtccgacccg gaagccctgg ccctggaggc ccggaccctg ggatatgtgg gcccgggaca
     1741 ggtcgtcgtc cggctgggtt tcccggaagc atcgccggat cccggcgacc tgggaaccct
     1801 ggttcccttc gatccgcccc ggggcatgga ggacgccgag ctgaaggccg cgtccctggc
     1861 ggccgccctg gtcgcggctt tcgcgggttt cctgattcga tcggacaggc ggggatctcg
     1921 gcagcgacgg ggtccggaag ggcgcgtcag tcccgccgga acagcctccg cacgagggct
     1981 ttgagggctt cccaaatcct gcgaaatata ttcggacgcc gcatgcgctc cagccgcgcg
     2041 tatccctcct ccagttcgag gtaggacagg gcggagcgct ggacgttttc ctccagttcc
     2101 agttccaggc ggtcggcctt gtcgtccgcg tcgacgatca gggcgttgat ggttttccag
     2161 cccagccgct tggccgcagc cagcctcctg cccccggcga tcagccggtt gccgcgatcc
     2221 aggacgatgg gatgcagcag tccgtggaca ttcatgctgg cggccagggc gtctatgtcc
     2281 ccgggttcct tgcggaccct gcgtcggacc ttgatgcgat cgatggggac ctgcacgctg
     2341 ccctccgctc cttttctata ccgataagtt tagctcggct tacccggcta ttcaaggacg
     2401 ggaggggtcg tgtattccgc gggaggaggg ggcggagtgc cgggggccgg ctgggttccc
     2461 ggagcgggcg cggacggcgt ctgcggggcg gggttccgga gcagctgctc caggtccggc
     2521 agatcgatgg tcagggtcgt atctatgtcc acatctccga acacggaccc ctgacggctc
     2581 agggtctgca tgccccgatc cgcggcctgg gacgagaact ggtgcaggtc gcagtactgg
     2641 tggggctggg tgccctcgta atacaggagg cccaccagtc cctcgtcgca gtaatccgtg
     2701 ggcaggagac ccgatttcgc gcagaccttg acgtccacga ggccggcctg gggacggatg
     2761 aagtccttga agggcagacc ccggtggatg gcttccatgt agttggccca gagcacgccg
     2821 gctatggcgg ctcccgattg ggtgactccc agggagttgc cgggacggtc gaatccgaac
     2881 cagaccgccg tggtcatgta cggcgtgaag ccgacggtcc aggcgtccgc ccagttctgg
     2941 gtggtgcccg tctttcccgc ggagggtatc gtgtacttct tgccttcctc gtccttgtag
     3001 gtgaagagct tgccgcccgc ggtgggatag ttcagggtcc cgtccgttac ggtcctacgg
     3061 agcatgtccg tcatgacgta ggcgttcagg ggggagatca cctggatggc ggaacccttc
     3121 ttcttctgct gggtccggag ctccttctcc ggttccagga agatccggcc ctgccgatcc
     3181 tccacggaac gtatcgctat gggggtcacc tcccgaccat ggttcgcgaa gatggagtag
     3241 gcccgggcca tcttcagggg ggataccccg atgactccca gggccagggg gtagaggcgc
     3301 gggaaggtcc tgcggatctc cgcggggtcc gtgacatcca ggagggcggc ggcgcggttg
     3361 atcgccgcgt cgaagccgat ggcgtccaat accttgacgg aggggacgtt catggaccgg
     3421 gccagggcaa gccaggtcag gacggggccc ttccattcgc cccggtagtt caaggggatg
     3481 tacggagtgc cgtcgtcgtt gtagaagacc acgggagtgt cgtagatcag gcttccttcc
     3541 gtgaacttcc gggtgtcgat ggccgcggag tagtagaggg gcttgaaggc gctgcccggc
     3601 atcaacaggg actgggtggc ccggatcagc tggttggact ggtcgtactt cgatccgccc
     3661 acgacggcct tgacgtgccc cgtctcgttc tcgatggtga tcagggcgcc ttccacggtc
     3721 gccttctcca tctggaggcg tacctggtc
//
LOCUS       DAOTEI010000250         3715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_18541_length_3715_cov_4.846448, whole genome shotgun sequence.
ACCESSION   DAOTEI010000250 DAOTEI010000000
VERSION     DAOTEI010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18541_length_3715_cov_4.846448"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="P5313_16115"
     CDS             <1..458
                     /locus_tag="P5313_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRY81941.1"
                     /translation="LDVEVSYMYVDNCAMQLVRNPSQFDVIATSNMFGDILSDEASMI
                     TGSIGMLASASLGDSGPGLYEPIHGSAPDIAGSGTANPLAAILSAALLLRHSLALEPC
                     ARAVESAVSRVLAEGYRCADIASGDPSEKVVGTEAMGDRVVRVLGRKEP"
     gene            455..2146
                     /gene="ilvB"
                     /locus_tag="P5313_16120"
     CDS             455..2146
                     /gene="ilvB"
                     /locus_tag="P5313_16120"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRY81942.1"
                     /translation="MMMSGARIVIECLAEQGVDTVFGFPGGSVIGIYDELYRNADRIR
                     HILTSHEQGAAHAADGYARASGKTGVVIATSGPGATNLVTGLATAYMDSVPLVAITGN
                     VPTNLLGKDSFQEVDITGITMSVTKHNFIVKDVAALASTLRRAFDIARFGRPGPVLVD
                     IPKNLCSASANWEPLPLSADKSFVPAPPRGTRSATDGEVDEAAALIASSRRPFIVAGG
                     GVIASGASEALRELAERIDAPVALTLMGQGAFPADHPLCTGMVGMHGTRASNAAVRNS
                     DLLIAVGTRFSDRVVSDTSSFIRDTQILHLDIDPAEIGKNVPCFHSLVGDAGELLSRL
                     LPRLERGSRPGWRDRVDGWREEPPAGRSPDGTAPRAALEAIRAAVPRDTIVTTEVGQH
                     QIWTAQYFGFSVPRTFLSSGGLGTMGYGTGAAIGAQLARPDRQVVHIAGDGSFRMNFT
                     ELATMVRYGLPVLVVVMNNRTLGMVRQWQTMLCQRRYAQTDLDRPPDFVKLADAFGIR
                     AWRAEDLPSFRAALAEALSERSPALIDLRIGIDECVLPMVLPGKPIEEQIMEVVN"
     gene            2186..>3715
                     /locus_tag="P5313_16125"
     CDS             2186..>3715
                     /locus_tag="P5313_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY81943.1"
                     /translation="MGRDAYVETAAGKPGGTGNPDSRAGELETLGLLFDLARTLDRHV
                     DLRDALGPVLELLEIRAGLARGMVTLLDRAEGVLRIEEARGYSAEDILRGRYRLGEGI
                     VGRVFESGDPIVVPDLAEEPRFLNRAGARSRADLAGKAYYCVPIRQGGAVTGTLSAER
                     KVRAVSPGTGPGRAGDPEAAGRGREAADLDLLERAAALIADSAKLRERIRERSSRAGP
                     DPTGTEPLAAACAGDEALSGSLVGTSDALRAVLALVDRVAPSDATVLITGESGTGKEL
                     VAAEIHRKSRRAGGPFVKVNCAALPESIIESELFGHEKGAFTGAAGRRKGRFELAHGG
                     TVFLDEIGELSSQVQVKLLRVLQEREIERVGGSGAVKVDVRVIAATNRSLEEELAAGR
                     FREDLYYRLNVFPIRVPPLRERKSDIVLLADHFTEKYAARNGKAVKRISSPAIDLLTS
                     YHWPGNVRELENCIERAVILSTDMVIHSYHLPPSLQSAVSTRTEPTSTLDAALSRLEK
                     EL"
BASE COUNT          556 a         1292 c         1314 g          553 t
ORIGIN      
        1 ggctggacgt ggaggtctcg tacatgtatg tggacaactg cgccatgcag ctggtccgga
       61 atcccagcca gttcgacgtt atcgccacca gcaacatgtt cggggacatc ctgtcggacg
      121 aggcctccat gatcacgggc tccatcggga tgctggccag cgccagcctc ggcgactccg
      181 gtccggggct gtacgagccc atccacggct cggcgccgga catcgcgggc agcggaacgg
      241 ccaaccccct ggcggcgatc ctctcggcgg ccctgctcct ccggcattcc ctggccctgg
      301 agccctgcgc cagggccgtg gagtccgcgg tgtcccgggt cctggcggag ggataccgct
      361 gcgcggacat agcctccggg gatccctcgg agaaggtcgt gggcacggag gccatgggag
      421 accgggtcgt ccgggtcctg gggaggaagg aaccatgatg atgagcggcg cgcgaatcgt
      481 gatcgagtgt ttggcggaac agggggtgga cacggtcttc gggtttcccg ggggttcggt
      541 catcgggatc tacgacgagc tctaccggaa cgcggaccgg atccgccaca tcctgacctc
      601 ccacgagcag ggggccgccc acgcggcgga cggctacgcc cgggcctccg gcaagacggg
      661 ggtcgtgatc gccaccagcg ggcccggcgc cacgaacctg gtgacgggtc tggccacggc
      721 ctacatggat tcggttcccc tggtggcgat taccgggaac gttcccacga acctcctggg
      781 caaggactcc ttccaggagg tggacatcac ggggatcacc atgtccgtca ccaagcacaa
      841 cttcatcgtg aaggacgtcg ccgccctggc ctccaccctg cgtcgggcct tcgacatcgc
      901 ccggttcggc cgtcccgggc cggtcctggt ggacatcccc aagaacctct gctccgcctc
      961 cgcgaactgg gagcccctgc ccctgtcggc ggataagagc ttcgtgcccg ccccgccccg
     1021 gggaacccgg tcggcgaccg acggggaggt cgacgaggcg gccgccctga tcgcctcgtc
     1081 ccggcggccc ttcatcgtgg ccgggggcgg ggtcatcgcc tccggtgcgt ccgaggccct
     1141 ccgggagctg gcggaacgga tcgacgcccc cgtggccctg accctcatgg ggcagggcgc
     1201 cttccccgcc gatcatcccc tatgcacggg catggtgggc atgcacggca cccgggcctc
     1261 caacgcggca gtccgcaact cggacctcct catcgccgtg gggacccgct tttccgaccg
     1321 ggtggtcagc gacacctcga gcttcatccg ggacacccag atcctccacc tggacatcga
     1381 tcccgcggag atcggcaaga acgtgccctg tttccactcc ctggtggggg acgccgggga
     1441 gctcctgtcc cggctcctgc cgcggctgga gcgcggctcc cgccccgggt ggagggaccg
     1501 ggtggacggg tggcgggagg agcccccggc gggccggtcc cccgacggaa cggccccccg
     1561 ggccgccctg gaggcgatcc gggcggcggt tccccgggac acgatcgtga ccaccgaggt
     1621 cggccagcac cagatctgga cggcccagta cttcgggttc tccgttcccc ggaccttcct
     1681 gtcctccggg ggcctcggga ccatggggta cggcaccggc gccgccatcg gggcccagct
     1741 ggcccggccg gaccggcagg tggtccacat cgccggggac ggctccttcc gcatgaattt
     1801 cacggagctc gccaccatgg tgcgctacgg cctgcctgtc ctggtggtgg tgatgaacaa
     1861 ccgcaccctg gggatggtcc ggcagtggca gaccatgctc tgccagcggc gctacgccca
     1921 gacggacctg gaccggccgc cggatttcgt gaagctcgcg gacgccttcg gcatccgggc
     1981 ctggcgggcg gaggacctcc cgtccttccg ggcggccctc gcggaagccc tgtccgaacg
     2041 ttccccggcc ctgatcgacc ttcggatcgg gatcgacgag tgcgtcctgc ccatggtcct
     2101 gcccgggaag cccatagagg agcagatcat ggaggtggtc aattaggggg cttacatttt
     2161 catacattta tgtaactatt gatgcatggg acgggatgca tacgtagaaa cggccgcagg
     2221 gaaaccgggc gggaccggga atccggactc ccgggccggg gagctcgaga ccctcggtct
     2281 cctcttcgac ctggcccgga ccctggaccg ccatgtggac ctgagggacg ccctgggtcc
     2341 cgtgctggag ctcctggaaa tccgggcggg cctggcccgg ggcatggtga ccctcctgga
     2401 ccgggcggag ggcgtcctcc ggatcgagga agcccgggga tacagtgccg aggacatact
     2461 ccgggggaga taccgcctgg gcgagggcat cgtgggccgg gtcttcgaat ccggggaccc
     2521 gatcgtcgtg cccgacctcg ccgaggagcc ccgattcctg aaccgcgccg gggctcgttc
     2581 ccgagcggac ctggccggga aggcgtacta ttgcgtgccc atacggcagg ggggggcggt
     2641 cacgggcacc ctgagcgccg aacgcaaggt ccgcgccgta tcccccggga ccggaccggg
     2701 cagggccgga gacccggaag cggccgggcg gggccgggag gctgcggacc tggacctcct
     2761 ggagagggcg gccgccctga tcgcggactc cgccaaactc cgggaacgga tccgggagcg
     2821 gtcctcccgg gccggaccgg atccgaccgg gacggagcct ctcgccgcgg cctgcgcggg
     2881 ggacgaggcc ttatcgggct ccctggtcgg caccagcgac gccctgcggg ccgtcctggc
     2941 cctggtggac cgggtggccc ccagcgacgc cacggtcctg atcaccgggg agagcggcac
     3001 cggcaaggag ctcgtggcgg cggagatcca ccggaagtcc cggcgggcgg gcggcccctt
     3061 cgtcaaggtg aactgcgcgg ccctgccgga atcgatcatc gagagcgagc tcttcggcca
     3121 cgagaaaggg gccttcaccg gggccgcggg ccggcgcaag ggccgcttcg agctggccca
     3181 cgggggcacg gtcttcctgg acgagatcgg ggagctctcc tcccaggtcc aggtcaagct
     3241 cctccgggtc ctgcaggaga gggagatcga gcgggtgggc ggctccgggg cggtcaaggt
     3301 ggatgtccgc gtgatcgccg ccacgaaccg cagcctggag gaggagctgg ccgcgggtcg
     3361 cttccgggag gacctgtatt accggctcaa cgtctttccg atccgcgttc cgcccctccg
     3421 ggagcggaag agcgacatcg tccttctggc ggaccacttc acggagaagt acgcggcccg
     3481 gaacggcaag gcggtcaagc gcatctccag cccggccatc gacctcttga ccagctatca
     3541 ctggccgggc aatgtccggg agctggaaaa ctgcatcgag cgggcggtca tcctgtccac
     3601 ggacatggtc atccactcct atcacctgcc ccccagcctc cagtccgccg tgtcgacccg
     3661 gacggagccg acttccaccc tggacgccgc cctttcgcgc cttgagaagg agctg
//
LOCUS       DAOTEI010000251         3640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_19109_length_3640_cov_3.689540, whole genome shotgun sequence.
ACCESSION   DAOTEI010000251 DAOTEI010000000
VERSION     DAOTEI010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19109_length_3640_cov_3.689540"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="P5313_16130"
     CDS             complement(<1..1230)
                     /locus_tag="P5313_16130"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRY81944.1"
                     /translation="MARPLRPGTLLKATLIAALGSALLLGIGIGVAIASTRNIANVED
                     FTDFNPALPTRLLDIKGRLITEFFSEEKREIVAIKELPQHLIDAFIVREDQDFWKHHG
                     FSLRGIFRAAFGVITRRNLGGGSTITQQLAGTLYADRTDISLRRKIVELWWALQLERR
                     FSKQEILEMYLNRMIMGPGVYGVEAASKFFFGHSAREVSVAEAAILVIQLSSPTRYNP
                     FRNPTLARERSREILDQMISLDYIEKETADDAFASYWENFDYTRTATSAFYMRDDKAP
                     WFSEYVRRQLEGMLYGSLDIYKDGFTVHTTLDLDYQAFADRFMARGIEQANKEYLASS
                     GLRLKEAESTYVPIVEMLGLAFNLEPLFVSDSKVQNRSQEYYRNTLNPAIDSLALLFG
                     IGDLRTATKASYDQVRLQ"
     regulatory      1432..1592
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1633..2340
                     /locus_tag="P5313_16135"
     CDS             1633..2340
                     /locus_tag="P5313_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY81945.1"
                     /translation="MSESKEKEEGLLGSTSSRRDFLRIAGTGIAGAFVTLTLGCATMV
                     RTQDGKNLKVWATPSGAMVQDPNLCVGCRRCESACTIRNDGKASAYVARVKVSRNLNY
                     GVEGVSSAYALANGQHGNFRIVAETCRQCGQPKCGDACPAVAIKVDKKTGARVVDSRR
                     CVGCGACQDACPWHIATLDPETRISTKCSLCDGDPTCAKACPTGSIKFYTWEEAEKLL
                     WGADATSGASAVSGATA"
     gene            2357..>3640
                     /locus_tag="P5313_16140"
     CDS             2357..>3640
                     /locus_tag="P5313_16140"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY81946.1"
                     /translation="MSQIFGWTGKILRVDLSTGKTSIDDTMKYKDFIGGEGIGYKILW
                     DEVPAGTHPYDPANKLILGAGPLCGSGVPCSGRTTIVSLLPTSPFHGPGSSHMGGYFS
                     VEMKYAGWDAIVLEGKAPKPVWIRIEDNKVKIEDARWMWGKGIFDTMAMISSQMGKEA
                     QVAAIGPAGENQLNMAVIRTGTSHSAGGHGGILGAKNVKAVAVKGTGSVKIAAEKAKV
                     QELDKFMMSIIGANNQMVVPSHPQPWAEFNSPGSRWTGRPGLFWGAANPPVETGVCDP
                     KDMQSVGFRTQKAVFDLGAGAEKYTVRMGGCAHCPIRCHSQLKVPQLEKYGMDPYVSN
                     TCVGWSGPRGVMAAGGGIKEFDGNGADSSVVGNGIGTTMVDDLGVWCNYGQIGRDVKW
                     FHEKGVFKRVLPAEEYASIPWDLLLKGDPRFLIDFY"
BASE COUNT          673 a         1150 c         1208 g          609 t
ORIGIN      
        1 ctggaggcgt acctggtcgt agctggcctt cgtggcggtg cgcaggtccc cgatgccgaa
       61 gagcagggcc agggagtcga tcgccggatt cagggtgttc cggtagtact cctgggaacg
      121 gttctggacc ttggaatccg agacgaagag gggctccagg ttgaaggcca gacccagcat
      181 ctccacgatg gggacgtagg tggactcggc ctccttcagg cgcaggccgc tggaggccag
      241 gtactctttg ttggcctgtt cgatgccccg ggccatgaac cggtccgcga aggcctggta
      301 gtccaagtcc agggtggtgt gtacggtgaa accgtccttg tagatgtcca gggacccgta
      361 gagcatcccc tccagctggc gccggacgta ttcgctgaac cagggggctt tgtcgtcccg
      421 catgtagaag gcgctggtcg cggtgcgggt gtagtcgaag ttttcccagt acgaggcgaa
      481 tgcgtcgtcg gccgtttcct tctcgatgta atccagggag atcatctggt ccaggatctc
      541 ccggcttcgc tcccgggcca gggtggggtt tcggaagggg ttgtaacggg tgggactgga
      601 gagctggatg accaggatgg cagcctccgc gacggagacc tcccgggccg agtgtccgaa
      661 gaagaacttg ctggccgcct cgaccccgta gacccccgga cccatgatca tgcggttcag
      721 gtacatctcc aggatttcct gcttggagaa ccggcgctcc agctggaggg cccaccagag
      781 ttccacgatc ttccgccgga ggcttatgtc cgtgcggtcc gcgtacaggg tcccggccag
      841 ctgctgggtg atcgtggagc cgccgcccag gttgcgccgg gtgatgaccc cgaaggcggc
      901 gcggaagatc ccccgaaggc tgaacccgtg gtgcttccag aaatcctggt cctcccggac
      961 gatgaaggcg tcgatcaggt gctgggggag ctccttgatg gccacgatct cccgcttctc
     1021 ctccgagaag aattccgtga tcaggcgtcc cttgatatcc aggagccggg tcgggagggc
     1081 gggattgaag tccgtgaaat cctctacgtt ggcgatgttg cgggtggacg cgatggcgac
     1141 cccgatgccg atgcccagga ggagggcgga accgagggcc gcgatcaggg tcgctttcag
     1201 gagggtgccc ggacggagag gacgagccat gcccgtaggg tatccgaagc ctcgggaccc
     1261 ggtcaaggcg aggacagcgg ggaaggaggc cttgtaggga tggaacatct ctaataattg
     1321 gaaaagtata tatttaaaca tatagatata aaagaaattt atacttgaca ggatcgcttg
     1381 gatgctttta atctggccct ggcggggatc cagaagatac ccgccccgat cttttccatc
     1441 cgcctccgaa ccttgggatc taaacgcctc ctccgtgcgg cgcaggatcc cgggtcccta
     1501 gggaacggcg tcgccgctcc cttgagggtg cgcggggaaa tccgcgcgcc tcccgccgca
     1561 acggcatcgg aaaggagacg acgcgccgaa acccgccccg ggccctgcgg acccgcgggc
     1621 aaggagtgag cgatgagcga aagcaaggag aaagaggaag gcttgctggg ctccacgtcg
     1681 tcccgccgcg atttcctgag aatcgcgggt accggtatcg ccggggcctt cgtaaccctg
     1741 accctcgggt gcgccaccat ggtccgcacc caggacggca agaacctgaa ggtctgggcc
     1801 accccctcgg gggccatggt ccaggatccc aacctctgcg tcggctgccg gcgctgcgag
     1861 tccgcctgca cgatccggaa cgacggcaag gcctccgcct acgtggcccg ggtcaaggtt
     1921 tcccggaacc tgaactacgg cgtcgaaggg gtctcctccg cctacgccct ggcgaacggc
     1981 cagcacggca acttccgcat cgtggccgaa acctgccgcc agtgcggcca gcccaagtgc
     2041 ggcgacgcct gccccgcggt ggccatcaag gtcgacaaga agaccggggc gcgggtcgtg
     2101 gactcccgcc gctgcgtcgg ctgcggggcc tgccaggacg cctgtccctg gcacatcgcc
     2161 accctggatc ccgaaacccg gatttccacc aagtgcagcc tctgcgacgg cgaccccacc
     2221 tgcgccaagg cctgccccac cgggtccatc aagttctaca cctgggaaga ggccgagaag
     2281 cttctctggg gagcggacgc gaccagcggc gccagcgccg taagcggcgc caccgcctaa
     2341 cgcaaggagg tcaaggatgt ctcagatatt cggttggacc ggcaagatac tccgcgtcga
     2401 cctctcgacc gggaagacct cgatcgacga caccatgaag tacaaggact tcatcggcgg
     2461 cgagggcatc ggctataaga tcctgtggga cgaggtgccc gcgggaaccc acccctacga
     2521 ccccgcgaac aagctcatcc tgggcgcggg ccccctctgc ggctccggcg tgccctgctc
     2581 cggccgcacg accatcgtca gcctccttcc caccagcccc ttccacggcc ccggcagctc
     2641 ccacatgggc ggctacttct ccgtggagat gaagtacgct ggctgggacg ccatcgtcct
     2701 ggagggcaag gcccccaagc cggtctggat ccgcatcgag gacaacaagg tcaagatcga
     2761 ggacgcccgc tggatgtggg gcaaggggat cttcgacacc atggccatga tttctagcca
     2821 gatgggcaag gaagcccagg tggcggccat cgggcccgcg ggcgagaacc agctcaacat
     2881 ggccgtcatc cgcaccggga cctcccattc cgcgggcggg cacgggggca tcctgggtgc
     2941 caagaacgtg aaggccgtgg ccgtgaaggg cacgggttcc gtgaagatcg cggcggaaaa
     3001 ggctaaggtc caagagctcg acaagttcat gatgagcatc atcggggcca acaaccagat
     3061 ggtcgtcccg tcccatcccc agccctgggc ggaattcaat tctcccggtt ctcgctggac
     3121 gggccggccg ggcctcttct ggggcgccgc gaatcccccc gtggagacgg gcgtctgcga
     3181 tcccaaggac atgcagtccg tgggattccg gacccagaag gcggtcttcg acctgggcgc
     3241 cggggccgag aagtacacgg tccgcatggg cggctgcgcc cactgcccga ttcgctgcca
     3301 ctcccagctc aaggttcccc agctcgaaaa gtacgggatg gatccctacg tgtccaacac
     3361 ctgcgtcggc tggtcgggtc cccgaggggt catggccgcg ggcgggggta tcaaggagtt
     3421 cgacggcaac ggcgccgact cctccgtcgt gggcaacggc atcggcacca cgatggtgga
     3481 cgacctgggg gtctggtgca actacggaca gatcggccgg gacgtaaagt ggttccacga
     3541 gaagggcgtc ttcaagcgcg tcctgcccgc ggaggagtac gcgtccatcc cctgggacct
     3601 gttgctcaag ggagatccga ggttcctcat cgacttctac
//
LOCUS       DAOTEI010000252         3630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_19192_length_3630_cov_4.260699, whole genome shotgun sequence.
ACCESSION   DAOTEI010000252 DAOTEI010000000
VERSION     DAOTEI010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19192_length_3630_cov_4.260699"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..898
                     /gene="trmB"
                     /locus_tag="P5313_16145"
     CDS             134..898
                     /gene="trmB"
                     /locus_tag="P5313_16145"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRY81947.1"
                     /translation="MDDEPDTLNRNSAETVGPDSPAPAPRESVTALRRGIRSYALRAG
                     RMTEFQRTSFETLAARWCVPFRSGEPFVPETAFGRTAPFVVEIGFGMGVATAEIAAAL
                     PATNFLGIEVHTPGVGKLLSEIENRSLRNLRILHHDAVEFLEDCVRPGSVSGFHVFFP
                     DPWPKKRHHKRRLIQRPLTDLLASRLVPWGYLYFVTDWEEYALWARDALDSTPGLTNR
                     FPGGWADRAPWRPVTKFERRALTEGREIRELWYVKP"
     gene            complement(914..1729)
                     /locus_tag="P5313_16150"
     CDS             complement(914..1729)
                     /locus_tag="P5313_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81948.1"
                     /translation="MDDPAVDGYSLRLPDGYDPSRQYPLLLSLHSSGQVGGSVEEALF
                     HGPPSVIRRFPETSPESLRRGFILVSPHLTDGPYEERQWFDRLELLDHILVEIARGYR
                     VDLSRIYLVGYSTGGTGTWGYASRRPGQFAAVIPIAGYSRPEQEIRKPIVDDWSVFRE
                     LPVWTFYNASDRAVGFSHGRNAVAGIEALDGKPFQVLNHGISGPDPYRGSVVIETPAG
                     EVSGKRRIFSVYRIGIHSEGRIWDNPVLYEWLLSHSNPNYRSGRLAGLLGRDP"
     gene            complement(1901..2452)
                     /locus_tag="P5313_16155"
     CDS             complement(1901..2452)
                     /locus_tag="P5313_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HRY81949.1"
                     /translation="MDTTTYTAFDGHTLVASAEPREALRSIKRYLDGKPDRAVLIFDD
                     GSGAQIDFDFRGTEEDVLARAYPPAQKPGRGRPALGVVCGEVSLLPRHWDWLKRQGNN
                     ASATLRRLVDDAIRNEPASARAKRAMEAVDREMWALAGNHPGCEEASRALYARDFAKF
                     RDLIRGWPRDVSSHLSMLAGRAE"
     gene            2549..3364
                     /locus_tag="P5313_16160"
     CDS             2549..3364
                     /locus_tag="P5313_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY81950.1"
                     /translation="MSEMEQSSTAPGQRVEIRIPVRHNPVLKTIVERVNADEELYALW
                     TCMNVNAVTRLGMTDHGPIHFQIVSNIALKMLRQLLEKGVVPSVVRDHGLTEADAEVI
                     VVLASLLHDLGMSIQRADHEQYSLFVAQPIIDRLLDGLYSPYVRTVIRAETQHAIIAH
                     RSEGRPMTVEAGIVRVADALDMTKGRSRIPFEAGVVNIHSLSAASIEQIEILSGEDKP
                     IWIKIRMSNSAGVFQLDELLKEKLHGSGLEKFVEVEASIEGETEKKLVKIFRL"
     gene            complement(3374..>3630)
                     /locus_tag="P5313_16165"
     CDS             complement(3374..>3630)
                     /locus_tag="P5313_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81951.1"
                     /translation="LVFALDHVLQVSRWKPKSAELGFVALFTDSAGSYWFRVSRGFHT
                     ALNESLASLEALVDDIAATATHEEKEKAAALYRKLSGFFG"
BASE COUNT          651 a         1201 c         1149 g          629 t
ORIGIN      
        1 tgctcagaac cgtcgacgcg ccccggcagc cggaaaccct ggaccgtctg tacgcctcgc
       61 ccgggtggag gcaggaagcc gagaccctgg tcgcgtacgg gtacgacgcg gagagaaacc
      121 tgggagtatt ctcatggacg atgaacctga caccctgaac cggaattccg ccgaaaccgt
      181 gggacccgat tcacccgcgc cggctccgcg ggaatccgtc acggcccttc gccggggcat
      241 ccgttcctac gctctccgcg cggggcggat gacggaattc cagcgtactt ccttcgagac
      301 cctggccgcc cgctggtgcg ttcccttccg ctccggggag cccttcgtcc cggaaaccgc
      361 gttcggccgg acggccccgt tcgtagtgga aatcggcttc ggcatggggg tggccaccgc
      421 cgagatcgcg gcagcccttc cggccacgaa tttcctgggc atcgaggtgc atacccccgg
      481 ggtcggcaag ctcctctcgg aaatcgagaa ccggagcctg cggaacctcc gcatcctgca
      541 ccacgacgcg gtcgagtttc tcgaggactg cgtccgcccc ggttccgtct cgggattcca
      601 cgtgttcttc ccggatccct ggcccaagaa gcgccaccac aagcgccgcc tgatccagag
      661 gcccctgacg gatcttttgg cctcccgact tgtaccctgg ggctacctct acttcgtgac
      721 ggactgggag gaatacgccc tctgggcccg ggacgccctg gactccaccc cgggcctgac
      781 caaccggttc cccgggggct gggcggaccg ggctccctgg cgcccggtca ccaagttcga
      841 gcgccgggcc ctgacggagg gccgggagat ccgggagctg tggtacgtca aaccctaggt
      901 tcgaggcacc gggtcacgga tcccgtccga ggagccccgc gagccggccg ctgcggtaat
      961 tcgggttcga atggctcaga agccattcgt agaggacggg gttgtcccag atccttccct
     1021 ccgagtggat gccgatccgg tacacggaaa agatccgtcg cttgccggag acctcgcccg
     1081 ccggggtttc gatgaccacg ctgcctcggt acggatccgg tcccgatatc ccgtggttca
     1141 ggacctggaa cggtttcccg tccagggcct cgatcccggc gaccgcgttc cgaccgtggc
     1201 tgaatcccac cgcccgatcg ctggcattgt agaaggtcca aaccggaagt tcccggaaca
     1261 ccgaccagtc gtccacgatc ggcttgcgga tttcctgttc cggtctgcta tatccggcga
     1321 tcgggatgac cgccgcaaat tgtccgggac gccggctcgc gtatccccag gtccccgtac
     1381 cgcccgtgga gtatccgacc agatagatcc tcgataggtc cacccgatat ccgcgggcga
     1441 tctcgacgag gatgtggtcc agaagttcga ggcggtcgaa ccattgacgt tcttcgtacg
     1501 ggccgtccgt aaggtgaggg ctgaccagga tgaagccccg ccgcagggat tcgggactgg
     1561 tttccgggaa ccggcggata acgctcggag ggccgtggaa gagcgcttcc tcgacggagc
     1621 ctcctacctg tccgctcgag tggagggaca ggagcaaagg atattggcgg gacggatcgt
     1681 atccgtcggg caggcggagg gaatacccgt ccactgccgg atcatccata cataccgtga
     1741 tgaattcccc ggcggttccg gccggtgagc gtggttccgg tccctcagcg gaaaccggaa
     1801 cgaccaggag ggcggcggca ataagaaccg ggaacttcat ggtacgcctc acgtctcagc
     1861 atacgcccgg cccgccggaa ccacaaggga ggggtcccct tcattctgcc cgccccgcga
     1921 gcatcgacaa atgggaggaa acatcccgag gccagccgcg gatcagatcg cggaacttcg
     1981 cgaaatcccg ggcgtacagg gcccgggacg cctcctcgca ccccgggtga ttgccggcca
     2041 gcgcccacat ctcccgatcc acggcctcca tcgcacgttt tgcccgggcc gaggccggct
     2101 cgttccggat tgcgtcgtcc acaaggcggc gcagtgtcgc ggacgcgttg tttccctgcc
     2161 gcttcagcca gtcccagtgt cggggcagga gggacacttc cccgcaaacg actccaagtg
     2221 cgggcctgcc tcggcccggc ttctgggcgg gcgggtaggc ccgggccagc acgtcctcct
     2281 cggtgccccg gaaatcgaag tcgatctggg ctcccgaccc gtcgtcgaag atgaggacag
     2341 cccgatccgg tttcccgtcc aggtaccgct tgatcgagcg taaggcttcc cggggctcgg
     2401 cggaagccac gagggtgtgg ccgtcgaagg ccgtgtaggt ggttgtatcc atgggcgcaa
     2461 tattataccc gggtatattt attgtcaata agatccgggt ataaatatag ggctttcccg
     2521 tacttccgct ttccgatact ctataggtat gagtgaaatg gaacaatcgt ctactgcccc
     2581 cggccagcgc gtggagatcc gcattcccgt gcgccacaat cccgtgctca agacgatcgt
     2641 cgagagagtc aacgccgacg aggagctcta cgccctgtgg acctgcatga acgtgaacgc
     2701 cgtgacccgc ctcgggatga cggaccacgg cccgatccac ttccagatcg tctccaacat
     2761 agctctgaag atgctccgtc agctcctgga gaagggcgtc gtacccagcg tggtccggga
     2821 ccacggcctt acggaggcgg acgcggaggt catcgtcgtc ctggcttccc tgctccacga
     2881 cctggggatg tccatccaga gggcggacca cgagcagtac tccctcttcg tggcccagcc
     2941 catcatcgac cgactgctgg acggactgta ctctccctac gtccggacgg tcatccgcgc
     3001 ggagacccag cacgccatca tcgcccaccg cagcgaggga cgacccatga ctgtggaggc
     3061 ggggatcgtc cgggtggcgg acgccttgga catgaccaag ggccggtccc ggataccctt
     3121 cgaggcgggg gtcgtgaaca tccactccct gtccgcggcc tccatcgagc agatcgagat
     3181 cctgtccggg gaggacaagc cgatctggat caagatccgc atgagcaact ccgcgggggt
     3241 cttccagctg gacgaactcc tgaaggaaaa gctccacggc tcgggcctgg agaagttcgt
     3301 ggaggtggaa gcctccatcg agggagagac cgaaaagaag ctcgtgaaga tcttccggct
     3361 ctaggcccgg ttactacccg aagaatcccg agagcttgcg gtacagggcc gcggcctttt
     3421 ccttctcctc gtgggtagcg gtcgccgcga tgtcgtccac cagggcttcc agggaagcca
     3481 ggctctcgtt cagggccgta tggaagcccc gggatacgcg gaaccagtag cttcccgcgg
     3541 agtccgtgaa gagcgccacg aatccgagct ccgcgctctt gggcttccag cgggacacct
     3601 gcaggacgtg gtccagggcg aaaaccaggg
//
LOCUS       DAOTEI010000253         3608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_19366_length_3608_cov_3.884323, whole genome shotgun sequence.
ACCESSION   DAOTEI010000253 DAOTEI010000000
VERSION     DAOTEI010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19366_length_3608_cov_3.884323"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1234)
                     /locus_tag="P5313_16170"
     CDS             complement(41..1234)
                     /locus_tag="P5313_16170"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRY81952.1"
                     /translation="MRKTLAVLLVLLTLGPGLAFAQNRTSLNIQCNEVGAEVYINGRL
                     AGKTTPNFSFLVPQGNVQVRVQKNGFQTFETVVRAGTSPITLNVTLQRIGAVVTPPPP
                     PPQNYNLLVNANVAGAEVILNNVSAGRVPFSASLAPGSYTLVVRAPGYTDYVENLVIS
                     GNRTVNAVLQGAFAAVTVVSNVQGADVIINEGQPFSQRGKAPFTANLPMGGAPYSIRV
                     TAPGYLDFNQTVSVTGPVTVTANLQPAMVPLVLNNLRPGAEIFLNGARIGVANSATFT
                     AQVAPGTYTVTIRLGGFLDFTTQVTVGAAAPQTIAPVFQPQPASYSFNIPANIQNPDM
                     KGNPWSQIRLFIDGVAQKDFRGQVSPGRHTIRIVSGAFAFETSFDFEAGKTYSFEPFL
                     GLNIR"
     gene            1533..2180
                     /locus_tag="P5313_16175"
     CDS             1533..2180
                     /locus_tag="P5313_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81953.1"
                     /translation="MNKRLIAVLAILTVAAAGAFAQIGLGLNAAIYSDTKMSFSEFGD
                     RLRNGEGVFYGPFIELALGKTALGLSGNFSFYEEDWSYDPMNPWYVPMVDFDLSLYAQ
                     GHLIKYRAFLDPFLEVGLGIMGKDYANEIDDPDLDNPIMATTYFQAGGGFGINLGGLG
                     IFIKGLYLFPMGTVKSSVYVDDDGDPFTPPLEITYDLATYPLRRLKLIAGAKIIL"
     gene            2416..2775
                     /locus_tag="P5313_16180"
     CDS             2416..2775
                     /locus_tag="P5313_16180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY81954.1"
                     /translation="MPEAPIQRLKVYQLAHALTLDLYRATRGFPADEKFGLTSQIRRA
                     ASSINANLMEGSHRRSRLDFASFIGIARGSCAELTYHLILARDLGYLNETDFRRYSER
                     MYILGSMLCGLYKKVKK"
     gene            2857..3603
                     /locus_tag="P5313_16185"
     CDS             2857..3603
                     /locus_tag="P5313_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRY81955.1"
                     /translation="MPSTSIDKAVLILKQLAVPPYEYKAGELARKLGMNRSSVYRILQ
                     VLKDCDLVISDKAGDYYKVGPEMYHIGTTYLYNNNFHSKLIDILSEISDIIKESVGMA
                     VMDGDKIMSLIEIEVHQPMKLNDVPGRYYPPNKGNYGKVLMAYQDPEYIERTLSGRVF
                     EKTFYNTLTTKEELLAEYARIRDRGYSFSVDELGIDILGVGIPIFGEKGRIKACVAVG
                     FYRSEGWEDKLHRVKDILLGYQGQIERNLP"
BASE COUNT          712 a         1031 c         1137 g          728 t
ORIGIN      
        1 acgccttttt ggcgccaatt ttaatccaac ctgccgccga tcaccggatg ttgagcccca
       61 ggaaaggctc gaaggaatag gtcttgccgg cctcgaagtc gaagctcgtc tcgaaggcga
      121 aggccccgga cacgatgcgg atcgtgtgcc gtccggggct cacctggccc cggaagtcct
      181 tctgggcgac cccgtcgatg aagagccgta tctggctcca gggattcccc ttcatgtccg
      241 ggttctggat gttcgcgggg atgttgaagg agtaggacgc gggctggggc tggaagaccg
      301 gggcgatggt ctggggcgcg gcggccccga cggtcacctg ggtcgtgaag tccaggaaac
      361 ctcccaggcg gatcgtcacc gtgtaggtcc ccggggcgac ctgggcggtg aaggtggccg
      421 agttggcgac tccgatccgg gcgccgttca ggaagatctc cgccccgggc cggaggttgt
      481 tgaggaccag gggcaccatg gcgggctgga ggttggcggt gacggtcacg gggcccgtta
      541 cggagacggt ctggttgaag tccaggtagc ccggagcggt gacccggatg gagtagggcg
      601 ccccgcccat gggcaggttg gccgtgaagg gggccttgcc gcgctgggag aagggctggc
      661 cctcgttgat gatcacgtcc gcgccctgca cgttggagac gaccgtgacc gccgcgaagg
      721 ctccctggag gaccgcgttg accgtccggt tgccggagat caccaggttc tccacgtagt
      781 ccgtgtaccc cggggcgcgc acgaccaggg tgtaggaccc gggggccagg gaggccgaga
      841 agggtacccg gccggcgctg acgttgttga ggatgacctc ggccccggcc acgttggcgt
      901 tcaccaggag gttgtagttc tggggagggg gaggcggcgg ggtgacgacc gcgccgatcc
      961 gctggagggt cacgttcagg gtgatcgggg aggtcccggc ccggacgacg gtctcgaagg
     1021 tctggaaccc gttcttctgg acccggacct ggacgttgcc ctggggcacc aggaaggaaa
     1081 agttcggggt ggtcttgccg gccaggcggc cgttgatgta gacctccgcg cccacctcgt
     1141 tgcactggat gttcagggac gtgcggttct gggcgaaggc caggcccggg cccagggtca
     1201 gaaggaccag gagcacggct aatgtctttc tcatcgattt cctccgtacg gtttggggaa
     1261 gcgcccccag ggtgcaatcc ctagtatacg acgcggagct cggagtttcc atcgggggcc
     1321 ggaaaaccgg caccgctttc ttccgcttcc gatagaaaag gagcgaacca gccccggtgt
     1381 tacgaagatt gtgaaatctc cattttgtcc cacattccat ctggtttttt ttgcccgatg
     1441 cgctatagta tcttcacgga aggcgcgggc gcaggaaccg gccttccatc gtgggccgga
     1501 agcgtccacg gtatcgatca ggaggttaag gaatgaacaa gagactaatc gcggtactcg
     1561 cgatcctcac ggtcgcggcc gcgggcgcct tcgcgcagat aggcctgggg ctcaacgccg
     1621 ccatctactc cgacacgaag atgagcttct cggagttcgg ggaccgcctc cggaacggcg
     1681 agggcgtctt ctacggtccc ttcatcgagc tggccctggg caagaccgcc ctgggactct
     1741 ccgggaactt ttccttttac gaagaggact ggtcgtacga tccaatgaat ccctggtatg
     1801 tccccatggt ggacttcgat ctgagcctct atgcccaggg ccacctgatc aagtaccgag
     1861 ccttccttga ccccttcctg gaggtgggac tggggatcat gggcaaggac tacgcgaacg
     1921 agattgatga cccggatctg gacaatccca tcatggccac cacctacttc caggccggcg
     1981 gaggcttcgg aatcaaccta gggggcctgg ggatcttcat caaggggctc taccttttcc
     2041 caatgggtac cgtaaagagt tcggtttacg tcgatgatga cggagatcct tttactccac
     2101 cgcttgagat tacctacgac ttggccacct accccctccg ccgcctcaag ctgatcgcgg
     2161 gggcgaagat tatcctgtaa acgtgaatgg gtgaatcgtg aataggtgaa tagggaggag
     2221 ggccttcttg tttacctaaa tcacgttcca tgagggctgc ggcgaaagtc gcggcctttt
     2281 ttatggaggt gaataggtga atgggtgaat tagtgaatgg gaagaagggc tcctattcac
     2341 ctatttaccc actcacctat tcacgttatc tgattctagt gcaatcaaaa aggcactcca
     2401 ggccgtatct cctgcatgcc ggaagctccg attcaaagac taaaagtcta tcagctagct
     2461 catgctctca cgttggatct atacagagcg actcgaggat ttccagccga tgaaaaattc
     2521 ggcctaactt cccagatccg tcgtgccgca tcgtctatta acgcaaacct tatggaggga
     2581 tcccatcgtc gatcccgttt ggatttcgcg tccttcatcg gtatcgcgcg cggttcctgt
     2641 gctgaactta cgtatcatct gatcctggca agagatctgg ggtatttgaa cgagacggat
     2701 ttccggcgtt attccgagcg gatgtacatt cttggttcga tgctttgcgg attgtataag
     2761 aaggtgaaga agtgaatcag tgaatgggtg aatagtcagt ccccattctc ccattcacct
     2821 attcacatat tcacctattc acggtacttt ccccgtatgc cctccaccag catcgacaag
     2881 gcggtcctca tcctcaagca gctggccgtg ccgccctacg agtacaaggc gggggagctg
     2941 gcccggaagc tggggatgaa ccggtcctcg gtctaccgga tcctccaggt cctgaaggac
     3001 tgcgacctgg tgatctccga caaggcgggg gactactaca aggtcggccc ggagatgtac
     3061 cacatcggga ccacctacct ctacaacaac aacttccact cgaagctgat cgacatcctc
     3121 tcggagatct cggacatcat caaggagtcc gtgggcatgg cggtgatgga cggggacaag
     3181 atcatgtccc tgatcgagat cgaggtccac cagcccatga agctgaacga cgtccccggg
     3241 cgctattatc cgcccaacaa gggcaactac ggcaaggtcc tgatggctta ccaggacccg
     3301 gagtacatcg agcggaccct ctccggccgg gtcttcgaga agaccttcta caacaccctg
     3361 accaccaagg aagagctcct ggcggagtac gcccggatcc gcgaccgggg ctactccttc
     3421 agcgtggacg agttggggat cgacatcctg ggggtcggga tccccatctt cggggagaag
     3481 ggccggatca aggcctgcgt ggccgtgggc ttctaccgca gcgagggctg ggaggacaaa
     3541 ctccaccggg tcaaggacat actcctgggc taccaggggc agatcgagcg gaaccttccc
     3601 tagcgggg
//
LOCUS       DAOTEI010000254         3513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_20203_length_3513_cov_5.432909, whole genome shotgun sequence.
ACCESSION   DAOTEI010000254 DAOTEI010000000
VERSION     DAOTEI010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20203_length_3513_cov_5.432909"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..386)
                     /locus_tag="P5313_16190"
     CDS             complement(27..386)
                     /locus_tag="P5313_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY81956.1"
                     /translation="MKPGPELPAVNRVTRHAVLGDLEPLREVTIWFEDRAVKAREGEP
                     ILSALIASGVGILRYTKNGSPRRMFCGIGRCTDCVMSVDGVPGVRTCVTAVRDGMRVE
                     RQEGVGRWAGEDGRDGA"
     gene            complement(383..1261)
                     /gene="dapA"
                     /locus_tag="P5313_16195"
     CDS             complement(383..1261)
                     /gene="dapA"
                     /locus_tag="P5313_16195"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRY81957.1"
                     /translation="MEYGRLIVALATPFRPDLEVDYDKTAELARRLVAEGATAFVSAG
                     TTGESPTLSREERIELIKTLKKAVRVPIIANVGTNDTRASIRNAKDAEEAGVDGLLLV
                     VPYYNKPDQGMLYDHFKAIAESVKIPSMLYNVPGRTAASVRPETVVKLSREVPNIRYV
                     KEASGDLEALTQIVRDSAPGFRAYTGDDSMTLPSLAVGAYGVVSVSGHVIGKQMKEMI
                     EAYVAGYVVKAAELHGKLLPLNRALFLQPNPVPLKAALKLCGFDAGSLRPPLKDASLE
                     VVQALKKALLDLGLLP"
     gene            complement(1331..1603)
                     /locus_tag="P5313_16200"
     CDS             complement(1331..1603)
                     /locus_tag="P5313_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY81958.1"
                     /translation="MDREENYICRCEEVTEEEIRAAIRGGARSVQEIKRWTRSGMGIC
                     QGRTCRRLVERILAEELGLSPGEAEPSSFRPPVRPVSLDSMDGSKE"
     gene            complement(1642..2367)
                     /locus_tag="P5313_16205"
     CDS             complement(1642..2367)
                     /locus_tag="P5313_16205"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY81959.1"
                     /translation="MKQPLDERELIVLECIRDSAEPLGSWSLVEHLEDRGYRVSSASI
                     GRILYRLEQRGYVEGQGNRGRIITKAGVTAIGKTKAFNSMDSHRKDLENLINSKVLED
                     FIMVLQARKAIERETARLAAENITEAGKRELHAILEEQEVKARRGESIAEIDIAFHRG
                     IAHASRNPALLALYGILSTMGQQSELFEFVRSRVNAPYRPAHRSILEAICSHDPDEAE
                     KNMIRHMDALIEDVTKYWDAYRD"
     gene            complement(2348..>3513)
                     /locus_tag="P5313_16210"
     CDS             complement(2348..>3513)
                     /locus_tag="P5313_16210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY81960.1"
                     /translation="KRGRSVVLVDKVVMGAGASGSCDDMILLQSKKPGILLEMAFRSL
                     ELFRDLEKELPCDIEFDTRGGTILIETPEELAVMEDFVASQNRHGLAVEIVDARRIRE
                     LHPHVTDRVIASTYSARDSQVNPMLLMKGFFLGATAKGLTFLRRSRPESLSPRNGRWE
                     IKLSTGETLETGSVVNAAGAWANEICGLLGLEFPIAPRKGQVLVTEQLPPLGATNVWS
                     AQYIVSKLRPAGVPAPGAAPWMSGPEEARKAADFGLGFAFTGTHSGNYLIGSTRENAG
                     FDKTTVPEALGLLARQVIHYFPVMKDVHFIRTFAGLRPSTPDGMPVIGEVPGFPGFFM
                     AAGHEGDGIALSPITGRVTADLVEGRKPEFSLESFRPERFLEGQGDLDEAASG"
BASE COUNT          514 a         1194 c         1169 g          636 t
ORIGIN      
        1 cgcccaccac gacggcgggc cgggtatcac gctccatccc gcccgtcctc tccggcccag
       61 cggccgacgc cttcctgtcg ttccacccgc atgccgtccc ggaccgcggt cacgcaggtc
      121 cgcactcccg gcaccccgtc cacgctcatg acgcagtccg tacagcggcc gattccgcag
      181 aacatccggc ggggagaccc gttcttggtg taccggagga tcccgacccc cgatgcgatc
      241 agggcggaga gtatgggttc cccctcccgg gccttcacgg cccggtcctc gaaccagatg
      301 gtcacctccc ggaggggttc caggtcgccc aggacggcgt gccgggtcac ccggttaacg
      361 gcgggcagtt ccggaccagg tttcatggaa ggagtcccag gtccaggagg gccttcttga
      421 gggcctggac gacctccagg gacgcgtcct tgaggggagg gcgcagggaa cccgcgtcga
      481 aaccgcagag cttcaaggcc gccttcaggg gcacgggatt gggctggagg aagagggccc
      541 gattgagggg gaggagcttc ccgtggagtt ccgcggcctt gaccacgtat ccggcgacgt
      601 aggcttcgat catctccttc atctgtttcc cgatcacgtg gcccgagacg ctcacgaccc
      661 cgtacgcccc gaccgccagg ctcgggaggg tcatggaatc gtccccggtg taggcccgga
      721 agcccggggc ggagtcccgg acgatctggg tcagcgcttc caggtccccg gaggcttcct
      781 tcacgtaccg gatgttcggc acctcccggg acagcttcac caccgtctcg gggcggacgc
      841 tggcggcggt ccggccgggg acgttgtaca gcatcgaggg gatcttcacg ctctcggcga
      901 tcgccttgaa gtggtcgtag agcatcccct ggtcgggctt gttgtagtag ggcaccacga
      961 gcaggaggcc gtccaccccg gcttcctcgg cgtccttcgc gttgcggatg gatgcccggg
     1021 tgtcgttggt cccgacgttc gcgatgatcg ggacgcgaac ggccttcttc agggtcttga
     1081 tcagttcgat cctctcctct ctggagaggg tgggggattc cccggtggtc cccgcggaga
     1141 cgaaggcggt ggctccctcc gcgaccaggc ggcgggccag ttcggcggtc ttgtcgtagt
     1201 ccacttccag gtccggccgg aagggtgtgg ccaaggccac gattagtcgt ccgtattcca
     1261 tcgcatcctc cgctcgttcg atactatata tagtaacacc ttgtccatag gtccggcgtc
     1321 cgggcccggt tcattccttg gacccgtcca tggaatccag ggacaccggc cggaccggcg
     1381 gccggaacga cgagggctcc gcctccccgg gggaaaggcc gagttcctcc gcgaggatcc
     1441 tttccacaag acgccggcag gtgcgcccct ggcagatccc catcccggag cgcgtccagc
     1501 gcttgatctc ctgtacggag cgtgccccgc cgcggatcgc cgcgcggatc tcctcctccg
     1561 tgacctcctc gcagcggcag atgtagttct cttcacggtc cattcgcagc tcctgggtcc
     1621 ggggtttcgg ggcccgccgg gtcagtcacg gtaggcgtcc cagtatttcg tcacgtcctc
     1681 gatgagggcg tccatgtgcc ggatcatgtt cttctccgcc tcgtcgggat cgtgggagca
     1741 gatcgcctcc aggatggacc ggtgggccgg ccgatacggg gcgttcaccc ggctccggac
     1801 gaactcgaag agctcggact gctggcccat cgtggaaagg atcccgtaca gggccaggag
     1861 ggcggggttg cgggaggcgt gggctatgcc ccggtggaag gcgatgtcga tctccgcgat
     1921 gctctccccc cgccgggcct tgacctcctg ctcttccagg atggcgtgca gttcccgttt
     1981 ccccgcctcg gtgatgttct ccgcggccag gcgggcggtc tcccgctcga tggccttgcg
     2041 cgcctggaga accatgatga agtcctccag gaccttgctg ttgatcaggt tctccaggtc
     2101 cttgcggtgg gagtccatgg agttgaaggc cttggtcttc cctatggcgg tcaccccggc
     2161 tttggtgatg atgcggccgc ggtttccctg cccctccacg tagccgcgct gttccaggcg
     2221 gtagaggatc ctcccgatgg aggcggagct gacccgatac cctcggtcct ccaggtgctc
     2281 gacgaggctc caggacccca ggggctccgc cgagtcgcgg atgcattcca gtacgatcag
     2341 ttccctctca tccagaggct gcttcatcca ggtctccttg cccttccagg aaccgttccg
     2401 gccggaaact ctccagggag aactccggct tccggccctc cacgaggtcg gccgtcaccc
     2461 gacccgtgat cggggacagg gcgatcccgt ccccctcgtg ccccgcggcc atgaagaagc
     2521 ccgggaatcc cggtacctcc ccgatcacgg gcatgccgtc cggcgtggac ggccggaggc
     2581 ccgcgaaggt ccgtatgaag tgcacgtcct tcatgaccgg gaagtaatgt atgacttgcc
     2641 gggccagtag gccaagggcc tcggggaccg tggtcttgtc gaaccccgcg ttttcccggg
     2701 tactgccgat caggtagttc ccgctgtggg tccccgtgaa ggcgaagccc aggccgaaat
     2761 ccgcggcctt ccgggcctct tcggggccgg acatccaggg ggcggccccg ggggccggga
     2821 ccccggcggg acgcagcttg gagacgatgt actgggcgct ccagacgttg gtggccccca
     2881 ggggcgggag ctgttcggtc accaggacct ggcccttccg gggggcgatg gggaactcga
     2941 ggcccaggag gccgcagatc tcgttggccc aggcccccgc ggcgttcacg accgacccgg
     3001 tctccagggt ttcccccgtg gagagcttga tctcccagcg tccgttccgg gggctcaggg
     3061 actccggccg gctccgacgg aggaaagtga gccccttggc ggtggccccc aggaagaagc
     3121 ccttcatgag gagcatgggg ttcacctggg agtcccgggc gctgtaggtg gaggcgatga
     3181 cccgatccgt cacgtggggg tggagctccc ggatccgtcg ggcgtccacg atctccaccg
     3241 caaggccgtg ccggttctgg ctggccacga agtcctccat gaccgccaac tcctcggggg
     3301 tctcgatgag gatggtcccc ccccgggtgt cgaactcgat gtcgcagggc agctccttct
     3361 cgaggtcccg gaaaagctcc aggctgcgga atgccatctc caggaggatg cccggcttct
     3421 tggactgcag aaggatcatg tcgtcgcagg agcccgaggc ccccgccccc atgaccacct
     3481 tgtccaccag gacgaccgat cgtccccgct tgc
//
LOCUS       DAOTEI010000255         3509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_20237_length_3509_cov_3.518529, whole genome shotgun sequence.
ACCESSION   DAOTEI010000255 DAOTEI010000000
VERSION     DAOTEI010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20237_length_3509_cov_3.518529"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..1511)
                     /locus_tag="P5313_16215"
     CDS             complement(387..1511)
                     /locus_tag="P5313_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81961.1"
                     /translation="MSIEVRLVQTPKDLRTFIQVPFSIYKDSPFWVPPLVFDELNSLR
                     KDKNPAFEFSDAEYWIAYKDGVPVGRIAGIVNRRAMEKWGNKDARFGWIDFIEDFEVA
                     KALVGTVEAWARSKGMEGLAGPLGFTDLDREGMLVEGFGELGTLATIYNHPYYPEYLS
                     RLGYAKEVDWVEYEVKTPKSIPEKALRVQELISKRSGIRLYEWKSKKDLIRRYAPAIF
                     DLINESYAKLYGTFVLTRAQIQAYIDQYLGFVDPRFLKVVVDSEDKLVCFAICMPSLS
                     RALQKARGALLPFGWFHLLRALRNPRTVDMYLVAVRSDWANRGAVAFLMTSLAESCIR
                     AGVQTNETNPELETNVEVQSIWKDFDRRQHKRRRAFRKAL"
     gene            complement(1521..1778)
                     /locus_tag="P5313_16220"
     CDS             complement(1521..1778)
                     /locus_tag="P5313_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81962.1"
                     /translation="MPLSDWKERKFDEVYRACLASLERQRRTDPSFTVDTARGVLEHL
                     YIQEGNDWTGRGEVQDIQIEASIAAYERFLSDWASEPAIRT"
     gene            complement(2073..2145)
                     /locus_tag="P5313_16225"
     tRNA            complement(2073..2145)
                     /locus_tag="P5313_16225"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2110..2112),aa:Met,seq:cat)
     gene            complement(2225..3325)
                     /gene="mnmA"
                     /locus_tag="P5313_16230"
     CDS             complement(2225..3325)
                     /gene="mnmA"
                     /locus_tag="P5313_16230"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRY81963.1"
                     /translation="MNDMPSVAVGLSGGVDSSVAAALLVQAGYRVSGLTMRIWDGPET
                     GQPGCRQGCYGPGEEEDVAACEALCSRLGIPYTVVDLSREYERTVLEYFRTEYLRGRT
                     PNPCVLCNHVMKFGFLLDRARIEGLRFDLFATGHYARVQERNGRPCLRTAADPSKDQS
                     YFLHRLKPEVLGKVLFPLGEKTKPEVRDIARSLGLEVSEKPESQDFVSGGDYSSVFSG
                     RESPPGDIVDTEGRVLGRHRGIHLYTIGQRRGLGVSSGPDPLYVLRLDPDRNHVVVGP
                     GEGLFSEGLEAEDAVLSDPSLEDAPFECRVRIRQNHAPAEARVTVRKGSARVLFRTPQ
                     RAVAPGQSAVFYDEAGFVLGGGVIEKALPREE"
     gene            complement(3345..>3509)
                     /gene="nadA"
                     /locus_tag="P5313_16235"
     CDS             complement(3345..>3509)
                     /gene="nadA"
                     /locus_tag="P5313_16235"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRY81964.1"
                     /translation="EFYTVGAAQHCLNMKKTRFADVVRALEGGKPVVDLDPGLMDRAR
                     LPIERMLALA"
BASE COUNT          632 a         1120 c         1128 g          629 t
ORIGIN      
        1 tccccggccc ggggcctgtt agatctcgcg gtcgaacggt tccggaccga gggcccctcg
       61 gatcccgagt gcggccccct ttatgtccgc aagagcgacg ccgagaccgc caaagaatga
      121 aaacaaagat tccaggattc accacggagc tcacggaggg cacggagaaa gagaccgcga
      181 gccaaaggaa agacaagata gacaaggaca aaatcggata aactcgatga atcgaggatg
      241 ggacattgcc atacctccat cctgtcgatc cgctcaaaat cttgtagatc ctgtcgaatc
      301 tctgcgaata ttttttcctt atgatctctc cgtgagttcc gtgccctccg tgatgagtcg
      361 tgaaaactcg cgccggaaat cccccgctag agcgccttcc ggaaggcccg gcggcgcttg
      421 tgctggcggc ggtcgaaatc cttccagatg ctctggacct cgacgttggt ctcgagctcg
      481 gggttcgtct cgttcgtctg gacccccgcc cggatgcagc tttccgccag ggaggtcatc
      541 aggaaggcca cggccccccg gttggcccag tccgagcgga cggcgaccag gtacatgtcc
      601 acggtccggg gattccgcag ggcccgcagg aggtggaacc agccgaaggg aagcaaggcc
      661 ccccgggcct tctggagggc ccgggacagg ctgggcatgc agatggcgaa gcacacgagc
      721 ttgtcctcgg aatccacgac gaccttgagg aaccggggat ccacgaagcc gaggtactgg
      781 tcgatgtaag cctggatctg ggcccgcgtg aggacgaagg taccgtagag cttggcgtag
      841 gactcgttga tcaggtcgaa gatggccggg gcgtagcggc ggatgaggtc cttcttggac
      901 ttccattcgt acaggcggat tccgctgcgt ttactgatga gctcctgtac cctcagggcc
      961 ttttccggga tgctcttggg agtcttgacc tcgtactcga cccaatccac ttccttggcg
     1021 taccccagcc tggaaaggta ttcgggatag taggggtggt tatagatcgt agccagggtg
     1081 cccagctccc cgaagccctc caccagcatc ccctcccggt ctaggtccgt gaagcccagg
     1141 ggccccgcca gaccttccat gcccttggat cgggcccagg cctccaccgt accgaccagg
     1201 gccttggcca cctcgaagtc ctcgatgaag tcgatccagc cgaaccgggc atccttgttg
     1261 ccccactttt ccatggcccg ccggttcacg atcccggcga tccgcccgac cgggacgccg
     1321 tccttgtagg ctatccagta ctccgcgtcg ctgaattcga aggcgggatt cttgtccttc
     1381 cgaagggagt tgagctcgtc gaagaccaga gggggaaccc agaaggggct gtccttgtag
     1441 atggagaacg gcacctggat gaaggtccgg agatccttcg gggtttggac gagcctcact
     1501 tcgatggaca taggcactcc ttatgtacgg attgcgggct ccgaggccca gtcggacagg
     1561 aagcgctcgt aggcggcgat ggaagcctcg atctggatgt cctggacctc gccccggccc
     1621 gtccagtcgt tcccttcctg gatgtacagg tgctccagga ccccccgggc ggtatcgacc
     1681 gtgaaggaag ggtccgtccg tctctgccgt tccaggctgg ccaggcaggc gcggtacacc
     1741 tcgtcgaatt tccgttcttt ccagtccgat agcggcatcc tgcctccggc gggccgcctg
     1801 gggcggcgga tcggtgggat tatataggag gccccgtggt tttggaaggg aggccggcgt
     1861 gtccaataag tcggttactt attggacacc cttgcagttt ctaacgttct ctcaagaacg
     1921 ttagaaactg ccgccctaaa accagttgtc caaaaagccg actgagcttt atggacagcc
     1981 cgggggccgg gctgccggat gcttcggcta tgtttcgctc ggccagtaaa aaaaccgcgc
     2041 ccgaagcccg ggggcttgag gcgcggtccg gatagcggcg gggggactcg aaccctcggc
     2101 acaacggata tgagccgttt gctctaccgt ctgagctacg ccgccaagcg cgtcgcggga
     2161 cgcgaaggaa tatataaccg gaacgaccgg agggtgtcaa gcgcgggacg gacggctcgg
     2221 cctcctactc ctcccgcggg agggccttct cgatgacccc gccgcccagc acgaatccgg
     2281 cctcgtcgta gaagaccgcg gactgccccg gggccaccgc ccgctggggg gtgcggaaga
     2341 ggacccgggc ggatcccttc cggaccgtga cccgagcttc cgcgggggcg tggttctgcc
     2401 gtatccggac ccggcactcg aagggggcgt cctccaggga ggggtccgac aggaccgcgt
     2461 cttccgcctc gagaccctcc gagaaaagtc cctccccggg acccacgacc acgtggttcc
     2521 ggtcgggatc cagccgcagg acgtacaggg ggtcgggtcc ggagctgacc cccaggcccc
     2581 gcctctggcc tatggtgtag aggtggattc cccgatggcg ccccaggacc cggccctcgg
     2641 tgtccacgat gtccccgggc gggctctccc gcccgctgaa taccgaagaa tagtcgccgc
     2701 cggagacgaa atcctggctc tcgggctttt cggacacctc cagtcccagg gatcgggcga
     2761 tgtcccggac ctcgggcttg gtcttctccc caagggggaa gagcaccttt cccaggacct
     2821 cgggtttcag tcgatggagg aaatagctct ggtccttgga gggatccgcg gcggtccgga
     2881 ggcagggccg gccgttcctc tcctggacgc gggcgtagtg ccccgtggcg aagaggtcga
     2941 agcggagacc ttcgatccgg gcccggtcca ggaggaaccc gaacttcatg acgtggttgc
     3001 acagcacgca ggggttgggg gtcctgccgc gcaggtactc ggtgcggaag tactctagga
     3061 cggtgcgttc gtactcccgg gaaaggtcca cgaccgtgta cggtatgccc aggcgggagc
     3121 acagggcctc gcaggccgct acgtcctcct cctccccggg gccgtagcag ccctgacggc
     3181 agcccggctg cccggtctcg ggaccgtccc agatgcgcat agtcaggccc gaaacccgat
     3241 acccggcctg gaccaaaagg gccgcggcca cggaggaatc gactcctccc gagaggccca
     3301 cggccacgga gggcatatcg ttcatgctct catccccggc ggccctacgc cagggccagc
     3361 atgcgttcga tgggtaacct tgcacggtcc atgagccccg gatccaggtc gacgacgggt
     3421 ttcccgcctt ccagggcccg gactacgtcg gcaaaccggg tcttcttcat gttgaggcag
     3481 tgctgggccg cccctacggt gtagaactc
//
LOCUS       DAOTEI010000256         3458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_20682_length_3458_cov_4.868058, whole genome shotgun sequence.
ACCESSION   DAOTEI010000256 DAOTEI010000000
VERSION     DAOTEI010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20682_length_3458_cov_4.868058"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="P5313_16240"
     CDS             complement(<1..591)
                     /locus_tag="P5313_16240"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRY81965.1"
                     /translation="MLDSKIVITCACTGAETTKAHNPALPVTPEEVADTAVACREAGA
                     AILHLHVRDENGKPTQDPKVFRKAIDLIRKRTDIVVEITTGGAVGDTAEERLRPVTEL
                     EPEMASLDCGTVNFGDDYIVNTLPVMRKFAQEFRRLKVRPTLECFDLGHVFSSRILIK
                     EGLLEPPYHYGLVMNVPGAVPFTLDTMESFVRVLPEW"
     gene            complement(805..2085)
                     /locus_tag="P5313_16245"
     CDS             complement(805..2085)
                     /locus_tag="P5313_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY81966.1"
                     /translation="MAASEYPKLNLSKSMSMFEEAQRLSPGGVMGIRRPYNFIQGEYP
                     IFIDRGYGGHIVDVDGNDYIDMLCAYGPVILGYDEKEINDAVKRQMDERGFCFSLVQP
                     AQNQLERALIDIIPCAEKVIPVKTGSDATTLAVRIARGYTDRAMVLRCGYHGWHDWCV
                     ENHGGVPEETLELTDEFEYGSLEALEAKLKEHAGKVACVIITPVGHPLAKPVMAPPKG
                     YLEGVKELVKKHGAVLVFDEIRTGFRAALGGAQERYGVTPDLGTFGKALANGYAISCV
                     AGKAEIMDVCQSKVFVSSTFFPNSLEMVAALKCLEILKREDVPNSIWARGTKFLERLR
                     KVVADSGCPATVSGIPPMPFVTFDKAPGDEEKAYKARREYFYTQTIRRGLFIQPFHHW
                     YIAHRHTDADLDKALWAIGEALELTMKKYPVGKV"
     gene            complement(2232..3272)
                     /locus_tag="P5313_16250"
     CDS             complement(2232..3272)
                     /locus_tag="P5313_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRY81967.1"
                     /translation="MSLGCKYGTHRVIEPVGAMPQTAQKINNDMSILYDNEILVDVIA
                     LNVDSASFTQIEQEAGGDVQKIGAKILEIVAARGKQQNPVTGSGGMLIGKVAKIGSAL
                     KDRDLKVGDKIASLVSLSLTPLKINKILKVHPEIDRVEIDGQAILFESGIYAKLPSDM
                     DEGLALAALDVAGAPAQAAKLVKPGDSVLVLGAGGKSGLLCLYESMKRAGPTGRVVAN
                     IHSERSRKVIQDLKIAHEIVVGDATKPVDLMNAVLKANKGREFDIAITCVNIAGCEMA
                     SILPVRDGGTVYFFSMATSFTRAALGAEGIGKDVNMIIGNGYTKDHAEITLWELRENP
                     ALRKVFEENYVK"
BASE COUNT          590 a         1068 c         1097 g          703 t
ORIGIN      
        1 ccactccggc aggacccgga cgaaggactc catcgtgtcc agggtgaagg gcaccgcccc
       61 ggggacgttc atcaccaggc cgtagtggta gggcggctcc agaagcccct ccttgatcag
      121 gatgcggctg gagaatacgt ggcccaggtc gaagcactcc agggtgggcc gcaccttgag
      181 acggcggaac tcctgggcga acttccgcat gaccggcagg gtgttcacga tgtagtcgtc
      241 cccgaagttg acggtcccgc agtccaggga ggccatctcg ggctccagct ccgtgaccgg
      301 ccgcaggcgt tcctccgcgg tgtcccccac ggcccctccg gtggtgatct ccaccacgat
      361 gtccgtgcgc ttgcggatca ggtcgatggc cttccggaag accttgggat cctgggtggg
      421 cttgccgttc tcgtcccgca cgtggaggtg gaggatggcg gcccccgcct cccggcaggc
      481 caccgcggtg tccgcgacct cctcgggcgt gaccggaagg gcggggttgt gtgccttggt
      541 ggtctccgcc cccgtgcagg cgcaggttat gacgatcttg gaatcgagca tacgcactcc
      601 tttaacgatt gaaccacaga ggcgggaaga agggcgtgga accagaaacc agacaggatt
      661 aacaggatga aagacggatg aacaagatag gaggggggaa ttgcgaattt cccaaacctg
      721 atcttgttaa tccgttctca atcttgtcca tcctgtctgt cctatggttc caaggccctt
      781 tccctccgtg tcctatcgtc ttctttaaac cttccccacc gggtacttct tcatggtcag
      841 ctccagggcc tcgccgatgg cccacagggc cttgtccagg tccgcgtccg tatggcgatg
      901 ggcgatgtac cagtgatgga agggctggat gaagagtccc cggcggatgg tctgggtgta
      961 gaaatactcc cgccgggcct tgtaggcttt ctcctcgtcc cccggggcct tgtcgaaggt
     1021 cacgaagggc atggggggga tgccgcttac cgtggcggga cagcccgagt cggcgacgac
     1081 cttccgcagg cgctccagga atttggtgcc ccgtgcccag atcgagttcg ggacgtcctc
     1141 gcgcttgagg atctccaggc acttgagggc cgccaccatc tccagggagt tcgggaagaa
     1201 ggtggaggag acgaagacct tggactggca gacgtccatg atctcggcct tgccggccac
     1261 gcaggagatg gcgtagccgt tggccagggc cttgccgaag gtacccaggt ccggggtgac
     1321 cccgtagcgc tcctgggcgc cgccgagggc ggcccggaag ccggtacgga tctcgtcgaa
     1381 cacgaggacg gcgccgtgtt tcttcacgag ctccttcacg ccctccaggt atcccttggg
     1441 cggggccatg accggcttgg ccagggggtg ccccacggga gtgatgatca cgcaggccac
     1501 cttgcccgcg tgctccttga gcttggcctc cagggcctcc agggacccgt actcgaactc
     1561 gtccgtgagc tccagggtct cctcgggcac cccgccgtgg ttctccacgc accagtcgtg
     1621 ccagccgtgg tagccgcagc gcaggaccat cgccctgtcc gtgtagcccc gggcgatccg
     1681 gaccgccagg gtcgtggcgt cggagcccgt cttcacgggg atgaccttct cggcgcaggg
     1741 gatgatgtcg atcaaggcgc gctccagctg gttctgcgcc ggctggacga ggctgaagca
     1801 aaagccccgc tcgtccatct ggcgcttgac cgcgtcgttg atctccttct cgtcgtagcc
     1861 caggatgacc gggccgtagg cgcagagcat gtcgatgtag tcgttgccgt ccacgtcgac
     1921 gatgtggccc ccgtaacccc ggtcgatgaa gatggggtac tccccttgga tgaagttgta
     1981 gggccgtcgg atgcccatga ccccgcccgg ggagagccgc tgggcctcct cgaacatgga
     2041 catggacttg gagaggttca gtttcggata ttcgctggct gccatgctgg ctccttcgca
     2101 aaggttttta ggactcacca cggaggacac agaggacacg gagaaatcga ggcgggattc
     2161 gataaatcat ctaattcctc atctctgctc cgtgttctcc gtgctctccg tggtttgttc
     2221 ctgcaaatac cttacttaac gtagttttct tcaaagacct tcctcagcgc cgggttttcc
     2281 cgaagctccc agagggtgat ctcggcgtgg tccttggtgt agccgttgcc gatgatcatg
     2341 ttcacgtcct tgccgatccc ctcggcgccc agggcggccc gggtgaagct ggtggccatg
     2401 ctgaagaagt agaccgtgcc gccgtcccgg accggcagga tgctggccat ctcgcagccc
     2461 gcgatgttca cgcaggtgat ggcgatgtcg aactcccggc ccttgttggc cttgagcacc
     2521 gcgttcatga ggtccacggg cttggtggcg tcgccgacca cgatctcgtg ggcgatcttg
     2581 aggtcctgga tgaccttccg agaccgctcg gagtggatgt tggccaccac gcggcccgtg
     2641 gggcccgcgc gcttcatgga ctcgtagagg cacaggaggc cggacttgcc gcccgcgccc
     2701 aggaccagta ccgagtcccc gggcttgacc agcttggccg cctgggccgg agcgcccgcc
     2761 acatccaggg ccgccagggc cagaccttcg tccatatccg aggggagttt ggcgtagatc
     2821 ccgctctcga agaggatggc ctggccgtcg atctcgaccc ggtcgatctc ggggtggacc
     2881 ttgaggatct tgttgatctt gaggggggtc agggaaaggg acacgaggga ggcgatcttg
     2941 tccccgacct tgaggtcccg gtccttcagg gccgagccga tcttggccac cttgccgatg
     3001 agcatcccgc cggatccggt tacgggattc tgctgcttgc cccgggccgc gacgatctcc
     3061 aggatcttgg cgccgatctt ctgcacgtcc ccgccggctt cctgctcgat ctgggtgaag
     3121 ctggccgagt cgacgttcag ggcgatcacg tccaccagga tctcgttgtc gtacaggatc
     3181 gacatgtcgt tgttgatctt ctgcgcggtc tggggcatcg caccgaccgg ttcgatcacc
     3241 cggtgcgtac cgtacttgca cccgaggctc ataggttcct ccttataaac ggaaacgata
     3301 tcgaaacgtt tcggggagag cggaaggctt ccccgcttga acccatggca gaatcaaggc
     3361 cagttacgat attttattat atattgttca acatgcaaac acgatgttca agatacaatc
     3421 acattatata ctattttcac gaatgcgcaa ccggtaaa
//
LOCUS       DAOTEI010000257         3433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_20915_length_3433_cov_5.693902, whole genome shotgun sequence.
ACCESSION   DAOTEI010000257 DAOTEI010000000
VERSION     DAOTEI010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20915_length_3433_cov_5.693902"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="P5313_16255"
     CDS             complement(<1..1251)
                     /locus_tag="P5313_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY81968.1"
                     /translation="MPRNDLTARPGHPRPFGAEPLDGGVNFAVFSRNALAMTLCLYEK
                     PEDGQAWREIRLDPHQNRTGDIWHVFVEGLAPGALYLYRAEGPFLPERGFRFNPNKAL
                     LDPYAKALTGEVTWDLSRARAYDPGRPEADLGFSEEDDAAYVPKCVVVDDQYDWEGDR
                     PLNYPLRHCVIYETHVRGLTAHSSSGVEHPGTYRGVVESIPRLKSLGITSLELLPVQE
                     FDSLDNPRKNPRTGEPLRQYWGYNTLAFFAPKSSYAASRVRGAQVAEFKDMVKALHRA
                     GIEVILDVVFNHTGEGNELGPTVSFRGLDNSIWYILDEDPRYYKNFSGCGNTLNCNHP
                     VVRNFILDCLHYWVVEMHVDGFRFDLGSILGRDSKGRLLENPPVIEQIAEDPILRQTK
                     IIAEAWDAGGAYQVGSFPGGRWAEW"
     gene            complement(1338..2537)
                     /locus_tag="P5313_16260"
     CDS             complement(1338..2537)
                     /locus_tag="P5313_16260"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRY81969.1"
                     /translation="MGLRRIGLLGGGQLGRMTIEEARKYPSYFAVLSPDFPSPGADLA
                     DEVVIGDLSDPEAVLDLGGRVDVVSYEIEHVRVDALKELEARGIPVLPGSAVLERIQD
                     KGLQKRLWDEAGLAQAPWVDLPENLSALPPADRAEALRSRAARVGGFPVVQKLRRGGY
                     DGRGVRVLSGPSDEPLPGPSFLEARVDFSKELAVVVVRAPGSEPAAYPCVEMVFDPAA
                     NLCDSVRAPADEPPEVLESALRAALRCVEALDRAAHAASPGGAGAVGVFAVELFLSRD
                     GRILVNEAAPRPHNSGHFTQDACAASQFEQYFRILAGLPPASTEQYRPARMLNILGDP
                     GASGVPEYEGLAEALAVPGVTVHLYGKREVRPWRKMGHLTAVADTPEEAARRAEAARS
                     RLRVLGG"
     gene            complement(2539..>3433)
                     /locus_tag="P5313_16265"
     CDS             complement(2539..>3433)
                     /locus_tag="P5313_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRY81970.1"
                     /translation="AFQALQYCICEMERRYSLLDGVGVRDIKSYNRRIKEREFAAETM
                     PYIVVVIDEFADLMATTGKELESTLARLAAMSRAVGIHLVLATQRPSIDVITGLIKAN
                     IPSRVAFMVASKFDSRIIIDAVGAEKLLGKGDMLVSTAQDAFPVRMQGAFVSEEEVER
                     VVGHVKTLGQPDYIDDEIFFDEDEEDGSPSLFDDEGDDPLFEKALEIVLQQGKASASY
                     LQRRLKIGYNRAARLVEMMEEKGIVGPSQGSKPRDVMRNPEVAGRPDAGRSVRPAPDS
                     ELGFGPAGPDGDAGGDTDGEG"
BASE COUNT          497 a         1177 c         1238 g          521 t
ORIGIN      
        1 ccattcggcc cagcgcccgc ccgggaagct gcccacctgg taggcccccc cggcgtccca
       61 ggcctcggcg atgatcttgg tctgcctcag gatgggatcc tcggcgatct gctcgatcac
      121 cggagggttt tccaggaggc gccccttgga gtcccggccc aggatggaac ccaggtcgaa
      181 gcggaacccg tccacgtgca tctccacgac ccagtagtgc aggcagtcca ggatgaagtt
      241 gcgcaccacc gggtggttgc agttcagggt gttgccgcag ccggagaagt tcttgtagta
      301 ccgggggtcc tcgtccagga tgtaccagat cgagttgtcc aggccccgga aggagaccgt
      361 gggcccgagc tcgttcccct ccccggtgtg gttgaagacc acgtccagga tcacctcgat
      421 cccggcccgg tgcagggcct tcaccatgtc cttgaactcc gcgacctggg cgccccggac
      481 ccgggaggcc gcgtaggagc tcttgggggc gaagaaggcc agggtgttgt agccccagta
      541 ctggcggagc ggttcgcccg tgcgggggtt cttgcgggga ttgtccaggg agtcgaactc
      601 ctggaccggc aggagctcga ggctcgtgat ccccagggac ttgagccggg ggatggactc
      661 gaccaccccc cggtaggtgc cgggatgctc caccccggag ctggagtggg cggtcaggcc
      721 ccggacgtgg gtctcgtaga tcacgcaatg gcgcagggga tagttgaggg gccggtcgcc
      781 ctcccagtcg tactggtcgt ccaccaccac gcacttgggc acgtaggccg cgtcgtcctc
      841 ctcggagaat ccgaggtccg cctccggccg gccggggtcg taggcccggg cgcgggagag
      901 gtcccaggtg acttccccgg tcaaggcctt ggcgtaggga tccaggaggg ccttgttcgg
      961 gttgaagcgg aagccccgct ccgggaggaa ggggccttcc gccctgtaca aatagagggc
     1021 cccgggagcc aggccctcca cgaagacgtg ccagatgtcc ccggtccggt tctggtgggg
     1081 gtccagccgg atctcccgcc aagcctgccc gtcctccggc ttctcgtaga ggcagagggt
     1141 catcgccagg gcgttgcggg aaaagacggc gaagttcacg cccccgtcca ggggctcggc
     1201 cccgaagggg cgggggtgtc cgggtcgcgc ggtcaggtcg ttccggggca agggggctcc
     1261 tcgatggggt acggcgcagt atagcacggc ggtgcgacct tggtggtacg aagcttgtga
     1321 cgggatcggc gccctcgtca gcccccgaga acccgaaggc gggaccgggc ggcctcggcc
     1381 cggcgggcgg cctcctcggg agtgtccgct acggcggtca ggtgccccat cttgcgccag
     1441 ggccggacct cccgcttgcc gtagagatgg accgtgaccc cgggtaccgc cagggcctcc
     1501 gccaggccct cgtactcggg gaccccggag gccccgggat cgcccaggat gttcagcatc
     1561 cgggccggcc ggtactgctc cgtcgaggcg ggaggaaggc ccgccaggat ccggaagtac
     1621 tgctcgaact ggctggccgc gcaggcgtcc tgtgtgaagt gcccggagtt gtggggccgc
     1681 ggggccgcct cgttgaccag gatccgtccg tcccgggaga ggaagagctc caccgcgaag
     1741 accccgacgg ccccggcgcc tccgggggat gcggcatggg ccgcccggtc cagggcctcc
     1801 acgcagcgca aggccgcgcg gagggcggat tccaggacct ccggcggctc gtccgcgggg
     1861 gcccggacgg agtcgcagag gttggccgcg ggatcgaaga ccatctcgac gcaggggtag
     1921 gcggccggct ccgagccggg agcgcggacg accaccaccg ccagctcctt ggagaaatcc
     1981 acccgggcct ccaggaagct tggcccggga aggggctcgt ccgaggggcc ggagaggacc
     2041 cggacgcccc ggccgtcgta gccgccccgg cgcagcttct ggaccaccgg aaagcctccg
     2101 acccgggcgg cccgggaacg gagggcctcc gctcggtcgg cggggggcaa ggcggaaaga
     2161 ttctcgggaa ggtcgaccca gggggcctgg gccaggcccg cctcgtccca gagccgcttc
     2221 tggaggccct tgtcctggat ccgctcgagg accgcggagc ccgggaggac ggggatcccg
     2281 cgggcctcca gctccttgag ggcgtccacg cggacgtgct cgatctcgta ggagacgacg
     2341 tccacccggc cgcccaggtc caggaccgcc tcgggatccg agaggtcccc gatcacgacc
     2401 tcgtccgcca ggtccgctcc gggcgaagga aagtccgggc tcaggacggc gaagtacgac
     2461 ggatacttcc gggcctcctc gatggtcatg cggcccagct ggccgccccc gaggaggccg
     2521 atccggcgga gtcccacctc agccctcccc gtccgtgtcc ccgcccgcgt ctccgtcggg
     2581 cccggcgggg ccgaatccga gctccgagtc cggtgcgggg cgtacggagc gtcccgcgtc
     2641 cggccggccg gccacctcgg ggttgcgcat gacgtcccgg ggtttggagc cctgggaggg
     2701 gccgacgatc cccttctcct ccatcatctc caccaggcgg gcggcccggt tgtacccgat
     2761 cttgagcctc cgctggaggt aggaggcgct ggccttgccc tgctggagga cgatctccag
     2821 ggccttctcg aacagggggt cgtccccctc gtcgtcgaag agggaggggc tcccgtcctc
     2881 ctcgtcctcg tcgaagaaga tctcgtcgtc gatgtagtcc ggctggccca gggtcttcac
     2941 gtggcccacg acccgctcca cctcctcctc ggagacaaag gccccctgca tgcgcaccgg
     3001 gaaggcgtcc tgggcggtgg acacgagcat gtcccccttg cccaggagct tttccgcccc
     3061 cacggcgtcg atgatgatcc gggagtcgaa cttgctggcc accatgaagg ccacccggct
     3121 ggggatgttg gccttgatga ggccggtgat cacgtcgatg gagggccgct gggtggccag
     3181 gaccaggtgg atccccacgg cccggctcat ggcggccagg cgggccaggg tgctctccag
     3241 ctccttgccc gtggtggcca tgaggtccgc gaactcgtcg atgaccacca cgatgtaggg
     3301 catcgtctcc gcggcgaact cccgctcctt gatccggcgg ttgtagctct tgatgtcccg
     3361 gacgccgacc ccgtccagga ggctgtagcg ccgctccatc tcgcagatgc agtactggag
     3421 ggcctggaag gcc
//
LOCUS       DAOTEI010000258         3429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_20948_length_3429_cov_4.920569, whole genome shotgun sequence.
ACCESSION   DAOTEI010000258 DAOTEI010000000
VERSION     DAOTEI010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20948_length_3429_cov_4.920569"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="P5313_16270"
     CDS             complement(<1..878)
                     /locus_tag="P5313_16270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY81971.1"
                     /translation="MGPKVRRLIGSLLALAIPAAGLGSLEWPVAGGVPVLTFGSHESG
                     RFHPSMTAGPEGAQVRAIGAGEISFSFDGDRLPSGLPCPLGGFVALSHPDGMISVYAR
                     LERGSLPRYLRAVSSGDILGRAGLSGFRTGRELSFTLYDRIKRQYLNPQVLLPPVRDD
                     RAPLVRGVYLSSGGKVLPLGETRSVRQGTYEIIADIQDPLAASDARPRAPYSIRLLVD
                     GKEAARYLYDAARAESGRLRFFGSARLDSRSYFLPDGRIRLGSFLLARGRTTIALIVS
                     DHAGNERETGGALQVD"
     gene            complement(879..1955)
                     /locus_tag="P5313_16275"
     CDS             complement(879..1955)
                     /locus_tag="P5313_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81972.1"
                     /translation="MRDVDERPNRLLYALIALAFLGYFFAAAEPLQKELALHPVWAGD
                     VSRDADPEPSPDPALAFSFHLGGRYGAVTPDGTRIWSRGAAFDAAVSDSLFLPYERES
                     KVLSAFTPGGDPAFRIQAEGWPFFRSGRLFLMSPEMDSVSEYSPEGKLLWSYDFPFKI
                     TDFDATESLAVAGLLDGSIECVDADGKSVLSFAPGGSRIEVTLGVALDPREEAIAAVC
                     GADRQRFILLVRKGGAFKVQYHRYLDSDYREPVTVRFTGDGRYVLYRQSEGIAVYDRQ
                     TGRESLLPLRAESFELASDASRGLEYLFFRAARTGGVVCFEPPARVIFSYPLPGGALW
                     TRHADRSLYLGSGAVLGRIDISEE"
     gene            2064..2465
                     /locus_tag="P5313_16280"
     CDS             2064..2465
                     /locus_tag="P5313_16280"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRY81973.1"
                     /translation="MELEELYREARKAAQNSYSPYSKFRVGAALLCGDGTVVTGANVE
                     NRSFGLTICAERGAITAAVSSGRRKFLALAICTPDAEYPVCLCGACRQFLSEFADPTL
                     PIAFGHGDSIGARTTMKELMPLDSLHELADR"
     gene            complement(2466..3272)
                     /locus_tag="P5313_16285"
     CDS             complement(2466..3272)
                     /locus_tag="P5313_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81974.1"
                     /translation="MGKITQEQRNLYFEKVKEYKKLIDARLSKEKTLQELLSSDDPGV
                     AYKRIAAAEEALNVASHYILINSVSVALLGIKNEDYLAEARRAIARAIKHFEDTVTAY
                     IDAPYSDYEKNLEAISDLSPQSRYQLLMKLGFAIQELEDAYGEMSRWKWAFIELWGKY
                     ATVAKNLLDLKSLPANLDFDSPHREVTHQHLSMVKRLFRDSADKYREKYEIFSNKAED
                     FRIAILFLNAIRRVHAVLGDRDDVEELKKKADIWTAKLESDIKKREAPKR"
BASE COUNT          599 a         1110 c         1137 g          583 t
ORIGIN      
        1 caatcgacct ggagcgcgcc gccggtttcc ctctcgtttc ccgcgtgatc ggagacgatg
       61 agggcgatgg tcgtccgccc ccgggccagg aggaagctgc ccagccggat cctgccgtcc
      121 ggcaggaaat aggaccggga gtccaggcgg gcggagccga agaaccgcaa ccgcccggat
      181 tccgcccggg cggcgtcgta caggtagcgt gcggcctcct tgccgtccac gagcaggcgt
      241 atggaatacg gggcccgggg gcgggcatcc gaggccgcca gagggtcctg aatgtccgcg
      301 atgatctcat aggttccctg gcggaccgac cgggtttccc ccaggggcag gaccttcccc
      361 cccgaggaga ggtagacccc ccggacgagg ggcgcccggt cgtcgcggac cgggggcagg
      421 aggacctggg gattcaggta ctgccgcttg atccggtcgt agagggtgaa cgaaagctcc
      481 cgccccgtcc ggaaacccga gagccctgcc cttccgagaa tatccccgga ggatacggcg
      541 cggaggtacc gcggcaggct tccccgctcc agccgggcgt atacgctgat cattccgtcc
      601 ggatggctaa gggccacgaa tccccccagg ggacagggta ggccggaggg cagcctgtcg
      661 ccgtcgaagg agaagctgat ttccccggcc ccgatggccc ggacctgcgc gccttcggga
      721 cccgcggtca tggagggatg gaaccggccc gattcatggc tgccgaaggt gaggaccggc
      781 acgcccccgg ccacgggcca ctccagggat cccaggcccg cggcggggat cgcgagcgcg
      841 aggagggacc ctatcagccg acgcaccttc ggtcccattc attcctccga gatgtcgatt
      901 cttcccagga cggccccaga acccaagtag aggctccgat ccgcgtgccg cgtccaaagg
      961 gcccctccgg gcaggggata cgaaaagatg acccgggcgg gcggctcgaa acagaccacc
     1021 cctccggtgc gggccgcccg gaagaacagg tactccaggc cccgggaggc gtcggaggcc
     1081 agttcgaagc tttccgcgcg caggggcagc agggattccc ggccggtttg acggtcgtag
     1141 acggcgatgc cctcggattg gcggtacagg acgtaccggc cgtccccggt gaaccgtacc
     1201 gtcacgggtt cccgataatc gctgtccagg tacctgtggt actggacctt gaaggcgccg
     1261 cccttccgga ccaggaggat gaacctctgc cggtccgccc cgcagaccgc cgcgatcgcc
     1321 tcttcccggg ggtccagggc tacccccagg gtgacctcga tccgacttcc cccgggcgcg
     1381 aagctgagga cggacttgcc gtcggcgtcc acgcattcga tgctcccgtc cagcaggccg
     1441 gccacggcca gggactccgt ggcgtcgaag tcggtgatct tgaacgggaa atcgtaggac
     1501 cagagcagct tgccctccgg tgaatactcg gagaccgaat ccatctccgg ggacatcagg
     1561 aacaatcgtc cggaccggaa aaagggccag ccctcggcct ggatccggaa ggcggggtcg
     1621 cctccggggg tgaaggcaga gagaaccttg gattcacgct cgtagggcag gaagagggaa
     1681 tcggacacgg ccgcgtcgaa ggccgccccc ctcgaccaga tccgcgtccc gtcgggagtc
     1741 accgctccgt accggccgcc cagatggaag gagaaggcaa gcgccggatc gggggacggc
     1801 tccggatccg cgtcccggga cacgtccccg gcccagacgg gatgcagggc gagctccttc
     1861 tgaaggggtt ccgcggcggc gaaaaaatac cccaggaacg cgagagcgat gagggcgtac
     1921 aggagcctgt tcggacgttc gtctacatcc cgcatcgcgg cctttccaaa tagggtcggt
     1981 ctttccagga ggcgcttaat ggtatatagt gaagcctccc gttgtcaacc gaaccaatcc
     2041 ctacgaagcg cgggagagca tcgatggaac tcgaggaact gtaccgggaa gcccgaaaag
     2101 ctgcgcaaaa ctcgtattcc ccgtattcga aattccgggt cggagcggcc ctgctctgcg
     2161 gggacgggac cgttgtcacc ggcgcgaacg tggagaaccg gtccttcggg ctgacgatct
     2221 gcgcggagcg cggagcgatc accgccgccg tttcctcggg gcgcaggaaa ttcctcgccc
     2281 tggccatctg cacccccgac gcggagtatc ccgtctgcct ctgcggggcc tgccgtcagt
     2341 tcctgagcga gttcgcggat ccgaccctgc ccatcgcctt cggccacgga gactccatcg
     2401 gagcccggac gaccatgaag gaattgatgc ccctggattc cctgcacgaa ctcgcggaca
     2461 ggtgatcagc gcttcggggc ttccctcttc ttgatgtcgc tctccagctt ggcggtccag
     2521 atgtccgcct tctttttcaa ttcctccacg tcgtcccgat cccccaggac ggcgtgcacc
     2581 cggcggatcg cgttcaggaa gaggatggcg atgcggaagt cctccgcctt gttggagaaa
     2641 atctcgtact tttcccggta cttgtccgcg gaatcccgga agagccgctt gaccatcgaa
     2701 aggtgctggt gggtgacctc ccggtgggga gagtcgaaat ccaggttggc ggggaggctc
     2761 ttcaggtcca ggaggttctt ggccaccgtg gcgtacttgc cccagagctc gatgaaggcc
     2821 cacttccacc gggacatctc cccgtaggcg tcctccagct cctgtatggc gaaacccagc
     2881 ttcatgagga gctggtagcg gctctgggga ctcaggtcgg agatggcttc caggttcttc
     2941 tcgtagtcgg agtagggagc gtcgatgtag gcggtgacgg tgtcctcgaa gtgcttgatg
     3001 gcccgggcga tggcgcggcg cgcctccgcg aggtagtcct cgttcttgat gcccaggagg
     3061 gccaccgaga cggagttgat caggatgtaa tgggaggcga cgttcagggc ctcctccgcg
     3121 gcggctatcc gcttgtacgc gacccccgga tcgtcggaac tcaggagctc ctgcagggtc
     3181 ttttccttag agagtcgggc gtcgatgagt ttcttgtatt ccttcacctt ctcgaagtag
     3241 aggttgcgct gttcctgcgt tatcttaccc atcgttttcc cgccaattac cgtagcgcct
     3301 tagaagctcc accgcgtggg cgtaggacac ttcggtcttg gaatcgcccg ccagcatgcg
     3361 cgctatctcc cgggccgtag cctcaccgga cagggccgcc aagcgggtga ccgtccggtc
     3421 ctgctggac
//
LOCUS       DAOTEI010000259         3395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_21271_length_3395_cov_5.050898, whole genome shotgun sequence.
ACCESSION   DAOTEI010000259 DAOTEI010000000
VERSION     DAOTEI010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21271_length_3395_cov_5.050898"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="P5313_16290"
     CDS             <1..955
                     /locus_tag="P5313_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010282298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81975.1"
                     /translation="AGFYYLFRGGLMNLERIGNTLASATTLIFTGLAVAFSFKTGLFN
                     IGASGQMLMGGLTATAIGLSVQMPRIVLLPLIVLAAASAGALWAAIPGFLKARFNVHE
                     VVATIMMNWIAYWTVYYTIPAYFKGTFLETESRRIPALASFRAEWLTNIFRGSYINMG
                     IFLAAAAVVVIAIILDKTTLGFELKAVGFNRHAAEYAGIHVPKNIVLSMSISGFLAGL
                     AGATYYLGYAVNMQIGVVPSAGIDGIAVALLGANSPWGVAASSVFFGILHSGKGFMNA
                     MTKVPPEIGDTIIAIIIYFAATGVLFTRLWERLTRKRSKEA"
     gene            957..1910
                     /locus_tag="P5313_16295"
     CDS             957..1910
                     /locus_tag="P5313_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY81976.1"
                     /translation="MQTLALIFPYAVAFTVPLLAASLGGLFSERSGVVNIGLEGLMVS
                     GSFTCAVVASRMETVLGPGHPGALWLGLGAAVVVGALFSLLHAFASISLKANQVISGT
                     AINMIAGSLTVFLARTMTGSGNVRIVLGFIPSDIPVLSDIPILGALFFTKTYATTWLT
                     AAILALAWFVIYKTPFGLRLRACGEHPQTADAAGINVYRMRYIAVSLSGALAGLAGGI
                     ILVTYSGEFNGSVAGLGFLALAALIFGQWNPLGILGATAFFGFAATIANVSQVIPQFS
                     RIPGLLLKAFPYVVTLIALVLFSKSSQAPRAAGEPYDQGKR"
     gene            complement(2041..2715)
                     /gene="deoC"
                     /locus_tag="P5313_16300"
     CDS             complement(2041..2715)
                     /gene="deoC"
                     /locus_tag="P5313_16300"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRY81977.1"
                     /translation="MEPAELAPFIDHTLLKPEATEDQIDVLCDEAAKHRFASVCVNST
                     WVERCARRLGGTGVKVCTVVGFPLGAMDSRSKAFEARTAVSNGASEVDMVMNIGALKA
                     GDLRAVREDMLAVRRACRVGIVLKVIIEACLLTDAEKVLACQVAKDVGADFVKTSTGF
                     SKSGATVADVALMRRTVGPSVGVKAAGGIRSFDDALAMLDAGANRLGTSQGVTLVSGQ
                     TPGGGY"
     gene            complement(2820..3311)
                     /locus_tag="P5313_16305"
     CDS             complement(2820..3311)
                     /locus_tag="P5313_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRY81978.1"
                     /translation="MSAEEGSAPADVVRNVAIGADHGAVEQKALLVRYLEVLGYRVTD
                     VGTFGTEKVDYPDIALAVCRKVKSGACERGIVLDGAGIGSCMAANKVRGIRAAMCYDL
                     RTVVNSREHNNANVLTLGGPLHTPDRLAEMVKVWLETRFAGGRHIVRINKIMALERGG
                     FEG"
BASE COUNT          480 a         1251 c         1059 g          605 t
ORIGIN      
        1 ggcgggattc tactacctct tccgcggcgg gctcatgaac ctggagcgga tcggcaacac
       61 cctggcctcc gccacgaccc tcatcttcac cggactggcc gtggccttct ccttcaagac
      121 cgggctgttc aacatcgggg cctccgggca gatgctgatg ggaggcctca cggccacggc
      181 catcggcctg tccgtccaga tgccgcggat cgtgctcctg cccctcatcg tcctggcggc
      241 cgcgtccgcc ggggccctct gggccgcgat accggggttc ctcaaggccc ggttcaacgt
      301 ccacgaggtg gtcgcgacca tcatgatgaa ctggatcgcc tactggaccg tgtactacac
      361 catcccggcc tacttcaagg gcaccttcct ggaaaccgag tcccgtcgca tccccgcgct
      421 cgcgtccttc cgggccgagt ggctcacgaa catcttccgg ggttcctaca tcaacatggg
      481 gatcttcctg gccgcggcgg ccgtggtcgt catcgcgatc atcctggaca agacgaccct
      541 gggcttcgag ctcaaggcgg tgggcttcaa ccgccacgcg gcggagtacg cgggcatcca
      601 cgtacccaag aacatcgtcc tgtccatgtc catctccggc ttcctggcgg gcctggccgg
      661 ggccacctac tacctgggct acgcggtgaa catgcagatc ggggtcgtgc cgtccgcggg
      721 catcgacggg atcgccgtgg ccctcctggg ggccaacagc ccctgggggg tggcggcctc
      781 ctccgtgttc ttcgggatcc tccattccgg gaagggcttc atgaacgcca tgaccaaggt
      841 gcccccggag atcggggaca ccatcatcgc catcatcatc tacttcgccg ccacgggggt
      901 gctcttcacc cgcctgtggg agcgcctcac gcggaagcgg agcaaggagg cctaggatgc
      961 agaccctcgc gttgatcttt ccctacgcgg tagccttcac cgtgcccctc ctggccgcgt
     1021 ccctgggggg cctgttcagc gagcgctcgg gggtggtgaa catcggcctg gaagggctca
     1081 tggtctccgg ctccttcacc tgcgccgtgg tggccagccg gatggagacg gtcctgggcc
     1141 ccgggcatcc cggcgccctc tggcttgggc tgggggcggc cgttgtggtg ggggcgctct
     1201 tctccctgct ccacgccttc gccagcatca gcctcaaggc caaccaggtc atcagcggca
     1261 cggccatcaa catgatcgcc ggctccctga ccgtcttcct ggcccggacc atgaccggtt
     1321 ccggcaacgt ccggatcgtc ctgggcttca tcccctcgga cattccggtc ctctcggaca
     1381 tcccgatcct gggggccctc tttttcacga agacctacgc caccacctgg cttacggccg
     1441 ccatcctggc cctggcctgg ttcgtcatct acaagactcc cttcggcctc cgcctgcggg
     1501 cctgcgggga gcatccccag accgcggacg cggcggggat caacgtgtac cggatgcgct
     1561 acatcgcggt gtcgctctcg ggcgccctgg cggggctggc gggggggatc atcctggtga
     1621 cctattccgg ggagttcaac ggaagcgtgg cgggtctcgg gttcctggcc ctggcggccc
     1681 tgatcttcgg gcagtggaat cccctgggca tcctgggggc cacggccttc ttcggcttcg
     1741 cggccaccat cgccaacgtc tcccaggtga ttccccagtt ctcccggata ccgggactcc
     1801 tattgaaggc cttcccctac gtggtcaccc tgatcgccct ggtcctgttc tccaagagca
     1861 gccaggcgcc ccgggcggcc ggggagccct acgaccaggg caaacgctag cgtaggacca
     1921 cgctgcgcgc ggtcctacgg caacgaagcg cgaagcgctt cgtcgagttt ccctaagccg
     1981 gcactataaa agcggccccc gttccacggg ggccgcgtcg tatccaaagg atcggaaccg
     2041 tcagtagccg cctcccgggg tctggccgct caccagggtg acgccctggc tggtgcccag
     2101 acggttcgcg ccggcgtcca gcatggccag ggcgtcgtcg aagctgcgga tcccgccggc
     2161 ggccttgacc cccacggagg gtcccacggt ccggcgcatc agcgcgacgt ccgcgaccgt
     2221 ggcgccggac ttggagaatc ccgtcgaggt cttcacgaaa tccgcgccca cgtccttggc
     2281 gacctggcag gccaggacct tctcggcgtc cgtgaggagg caggcctcga tgatgacctt
     2341 gaggacgatg ccgacgcggc aggcccggcg gaccgccagc atgtcctccc gcacggccct
     2401 gagatccccg gccttcaggg ccccgatgtt catgaccatg tcgacctcgg acgccccgtt
     2461 cgagacggcg gtgcgggctt cgaaggcctt cgaacggctg tccatggccc ccagggggaa
     2521 gcccacgacc gtgcagacct tgacccccgt cccgcccagg cggcgggcgc agcgctccac
     2581 ccaggtcgag ttcacgcaga cggaggcgaa gcggtgcttg gccgcctcgt cgcacagaac
     2641 gtcgatctgg tcttccgtgg cctcgggctt gagaagggtg tggtcgatga agggcgccag
     2701 ctcggccggt tccatccggg gccgggcggg cccctcgtcg ttcggaacct cggccgtccg
     2761 ggtccccatc ctcgccagga cctcctgcac gatcctctcg attacctttt cctcgtccgt
     2821 catccttcga agcctccgcg ctccagggcc atgatcttgt tgatcctgac gatatgccgg
     2881 cccccggcga accgggtctc cagccaaacc ttcaccatct ccgccaggcg gtccggcgtg
     2941 tgcaaggggc cgcccagggt aagcacgttc gcgttgttgt gctcccggct gttcaccacg
     3001 gtccggaggt cgtagcacat cgcggcgcgt atgccccgaa ccttgttcgc cgccatgcag
     3061 gacccgatgc ccgccccgtc caggacgatc ccgcgctcgc aggccccgct tttcaccttc
     3121 cggcacaccg ccagggcgat gtccgggtag tccaccttct cggtcccgaa ggtccccacg
     3181 tcggtcaccc ggtaccccag gacctcgagg tagcggacca ggagggcctt ctgctcgacg
     3241 gccccgtggt ccgcgccgat ggccacgttc cgcacgacgt ccgcgggcgc ggacccttcc
     3301 tcggcgctca ttcgtcccct cccatgtcga tccggtccac gatgccgcac accgcggtcc
     3361 gcacggtcac cgccccctcg tcccccacga cggtc
//
LOCUS       DAOTEI010000260         3224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_22953_length_3224_cov_5.218681, whole genome shotgun sequence.
ACCESSION   DAOTEI010000260 DAOTEI010000000
VERSION     DAOTEI010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22953_length_3224_cov_5.218681"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..750
                     /locus_tag="P5313_16310"
     CDS             127..750
                     /locus_tag="P5313_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81979.1"
                     /translation="MTSTRDFLRWYARSPLGMGAFVLSAAAGVGMGLAGVAVPAAVGI
                     GAGAFLAASAAAFASGAGGRAAARERDRDLDASLAAGIAAAAKDRDRIAALRIRDAEV
                     ASASAAFALAFGEYLDACRREGTRDPQADDAARDVLAALTAYLRELDGTAVERRFDLP
                     DADPFPEAKARTLAFLKERIAYVRERRLSVDGGLGGSDTLAIREELR"
     gene            747..2408
                     /locus_tag="P5313_16315"
     CDS             747..2408
                     /locus_tag="P5313_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81980.1"
                     /translation="MTNTLRRPHRAAALCVLLLAAVSAAALESRVATVDVTVSLRPDG
                     KGTVFYRLEWVVSSGTLGGFYFQGESFAPVWDSQRCWADLPGGSRVGLDIKNLGSGRY
                     DVLLAGGRRTGGTSFWNLTYGGDFAAADLVGRTTSPELGDLVRITWAPVEWDQMLDSR
                     TVRIVLPVAVSGPTLTDEEKRAIPLRTEEFVNRENRIDYYGSPGDDGRHYLTFLFYQE
                     GVPIRGTQELVLYLPADYLSMDLPEAGAASASGDGYEEAYEPYVWVGPREYWGTRPWV
                     AAPVFAGLAALALVLYARRLSAYRRAAAVLEGIAWAGDAWTPPRLQEGSYQVPGKVAE
                     DLHPVEAALLLELPLASVASIMLEGLKAQGIVEILDPDPLRIRILSARKAESEYEELF
                     LAAFDPEGKVLSGLMADFFEEAIKRLQEKIWDCDLEATRAFYRERLARAGREESGVEA
                     GGTSAAGRRYDPYWHGYWMVHSRPAYYESVRLPSDLGTGYADFMRSASCFSGCFSPPD
                     VGTVSACHSACHNACHDACHSACHSACHSACHSACHSACVSGGGH"
     gene            2408..>3224
                     /locus_tag="P5313_16320"
     CDS             2408..>3224
                     /locus_tag="P5313_16320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY81981.1"
                     /translation="MRSEGAAAGLVKLGWIAPFWRKVRSRLLLRPEDDVLILPPNRVY
                     KLNSTAKALVLFLEAGGRLDDFPGLNAGRARETDSFFRSLEAALSGRRPDADRVAYDF
                     SFTRLPVLAELAVTYRCNNRCRFCYAGCSAGTGCRRPGTAGAEEPASGRSEIEDLPAR
                     DLEKILRIFKEEAKVPFFSFTGGEPLLREDLPRLVRYAVRIELRTNLVTNGTLATPSL
                     ARDLFRAGLRTAQVSLEAPEEELHDDLCGAAGAWERSVAGIRALMEAGISVQTN"
BASE COUNT          430 a         1187 c         1118 g          489 t
ORIGIN      
        1 gaaccgcgcc cggaaccggc gggtggagat cacggtcctc aactgaggcc gccgcgcctg
       61 agtccggatg atccgatttc cccgccgagc ttgccagacc cggcgcaccg tagtacgctc
      121 ctagccatga cgagtacccg ggatttcctc cgatggtatg cacgatcccc cctgggaatg
      181 ggcgcctttg tcctctccgc ggcggcgggc gtcgggatgg gactggccgg cgtcgccgtg
      241 cccgccgcgg tcggcatcgg ggcgggggcc ttcctggccg cttccgccgc ggccttcgcc
      301 tccggggcgg ggggccgtgc cgcggcccgc gaacgggacc gggacctgga cgcctccctg
      361 gcggcgggga tcgcggccgc cgcgaaggac cgggaccgga tcgccgcgct gcgcatccgg
      421 gacgccgagg tcgcctcggc ctccgcggcc ttcgccctgg ccttcggcga gtacctggac
      481 gcctgccgac gggagggcac ccgggacccc caggcggacg acgcggcccg ggacgtcctg
      541 gcggccctca ccgcctacct ccgggaactg gacggcaccg ccgtggagcg ccgcttcgac
      601 ctgccggatg cggatccctt cccggaggcc aaggcccgga ccctggcctt cctgaaggaa
      661 cggatcgcct acgtccggga acgccgcctg tccgtggacg gcggcctcgg ggggagcgac
      721 accctggcca tacgggagga actccgatga cgaataccct tcggcggccc caccgcgccg
      781 cggcgctttg cgtacttttg ctcgccgccg tctcggcggc cgccctggaa tcccgggtag
      841 ccaccgtgga cgtcaccgtg agcctccggc cggacgggaa ggggacggtc ttctaccgcc
      901 tggaatgggt ggtgagctcc gggaccctgg gaggcttcta tttccagggc gagtccttcg
      961 cccccgtctg ggattcccag cgctgctggg cggacctacc cggaggttcc cgggtcggcc
     1021 tggacatcaa gaatctggga agcggacgct acgacgtact gctcgcgggt ggccgccgga
     1081 cgggggggac ctccttctgg aacctgacct acggagggga cttcgcggcc gcggatctgg
     1141 tcggcaggac cacgagcccc gaactcgggg acctggtccg catcacctgg gcccccgtgg
     1201 agtgggacca gatgctggat tcccggaccg tgcggatcgt ccttcccgtg gccgtatccg
     1261 gaccgaccct cacggacgag gagaagcggg ccatccccct gcggacggag gagttcgtca
     1321 accgggaaaa ccgcatcgac tactacggtt cccccgggga cgacggccgg cattacctga
     1381 ccttcctctt ctaccaggag ggggtcccga tccggggaac ccaggaactg gtcctctacc
     1441 ttcccgcgga ctaccttagc atggaccttc ccgaagccgg ggccgcgtcg gccagcgggg
     1501 acgggtacga ggaagcctac gagccctacg tatgggtcgg accccgggag tactggggca
     1561 cccggccctg ggtcgcggcg ccggtctttg ccgggctcgc ggccctggcc ctggtcctgt
     1621 acgcccggcg cctatcggcc taccggcggg ccgccgcggt gctggagggc atagcctggg
     1681 ccggggacgc ctggaccccg ccccgcctcc aggaaggctc ctaccaggtc cccggcaagg
     1741 tggccgagga cctccacccc gtggaggccg ctctcctcct ggagctcccc ctggccagcg
     1801 tggcctccat catgctggag ggactcaagg cccagggcat cgtcgagatc ctggaccccg
     1861 accccctgcg gatccgcatc ctctcggccc gcaaggccga gagcgagtac gaggagctct
     1921 tcctggccgc cttcgacccg gagggcaagg tcctgtcggg cctcatggcg gacttcttcg
     1981 aggaggccat caagcgcctc caggagaaga tctgggactg cgacctggag gccacccggg
     2041 ccttctaccg ggagcgcctg gcccgggccg gccgggagga gtccggggtc gaggcgggcg
     2101 ggacctccgc ggcgggccgg cggtatgacc cctactggca cgggtactgg atggtccatt
     2161 cccgccccgc ctattacgag tccgtgcgcc ttccctccga tctgggcacg gggtacgcgg
     2221 acttcatgcg gagtgcctcc tgtttctccg gctgcttctc cccgcccgac gtgggcaccg
     2281 tgtccgcctg ccactcggcc tgccacaacg cctgccacga cgcctgccac tcggcctgcc
     2341 acagcgcctg ccattccgcg tgccactcgg cctgccactc cgcctgcgtg tccgggggcg
     2401 gccactgatg cgttccgaag gagctgccgc aggcctcgtg aagctggggt ggatcgcccc
     2461 cttctggcgg aaggtccggt cccgcctcct cttgcgcccg gaggacgacg tcctcatcct
     2521 gccccccaac cgggtctaca agctcaattc cacggccaag gccctggtcc tcttcctgga
     2581 ggccgggggc cgcctggacg acttcccggg cctgaacgcc gggcgggccc gggagacgga
     2641 ctccttcttc cgctccctgg aagcggccct ctcggggcgc cggcccgacg cggaccgggt
     2701 ggcctacgac ttttccttca cccgcctgcc ggttctggcg gagctggcgg tcacgtaccg
     2761 ctgcaacaac cgctgccgct tctgctacgc cggatgttcc gcgggaacgg gctgtcgccg
     2821 ccccgggacc gcgggcgccg aggagcccgc gtccggccgc tccgagatcg aggaccttcc
     2881 ggcccgggac ctggagaaga tcctccggat cttcaaggag gaggccaagg tccccttctt
     2941 ctcctttacg ggcggggagc ccctcctgcg ggaggacctg ccccggctgg tccgatacgc
     3001 ggtccggatc gaactcagga ccaacctggt gaccaacggg accctcgcga ccccgtccct
     3061 cgcccgggac ctcttccggg cggggctccg gacggcccaa gtgagcctgg aagccccgga
     3121 ggaggagctc cacgacgacc tgtgcggggc cgccggggcc tgggagcgga gcgtggcggg
     3181 tatccgcgcc ctgatggagg cggggatctc ggtgcagacc aata
//
LOCUS       DAOTEI010000261         3133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_24056_length_3133_cov_4.051657, whole genome shotgun sequence.
ACCESSION   DAOTEI010000261 DAOTEI010000000
VERSION     DAOTEI010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24056_length_3133_cov_4.051657"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="P5313_16325"
     CDS             <1..850
                     /locus_tag="P5313_16325"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRY81982.1"
                     /translation="DFYDRVAHKKGELWHIADGSAWIAQRWGLGDDYWDHKDNKLWTR
                     GGYPVHHSNESAGQVGALISCFTNRDAQNHTHQNFIGSQLPIAIQKEIAGEIFGSPDA
                     LDPPQNYTRMNEFKARFVKWALVRGRLHDSLTLCNWMWPMAVSPLKERKYRGDTSLEA
                     QFFSAVTGVTKTEAELDKDAERVVTLFRALTVRAMGTVDMRGKHDAMNKWIYTPDKKD
                     GKEIPQFTKGTIQMEKDDLDKGLGMVYKQFGWDEKTGAPTKAGLEAIGLKDVADELGS
                     KGLLPA"
     gene            962..1558
                     /locus_tag="P5313_16330"
     CDS             962..1558
                     /locus_tag="P5313_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81983.1"
                     /translation="METSDVTASDAPSAVGTVRRDSAAGRLTLLSALRADAARNASLP
                     PAAAEIGQDAPSSAVPADPLDEIGGSGAEDLKRMDGRAETYFFSDLYMTRAYASYLYR
                     LAEKDLVGLVVETVREDSRLYPRPTPLDFFRGPPFTLAQDQLDDILTRIREREDCRDI
                     RECRASNGARYLYSTLHLTVAHAEALTEWNEVGQAENP"
     gene            1555..2286
                     /gene="fdhD"
                     /locus_tag="P5313_16335"
     CDS             1555..2286
                     /gene="fdhD"
                     /locus_tag="P5313_16335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRY81984.1"
                     /translation="MSDARPDLPLAPEYPVELTVNGTRLATFLCTPEHLDDLAAGNLF
                     TRGSLVDPGQIRTLGACPDLRIVAVTTRTPLGEESLGLSRVIASGCGAGLEFSEDFKN
                     APALPEGFSVELSSLRARARAMFEAAVQYRRTGGMHCASLAGRDGRTCVREDVGRHNA
                     VDKVIGRGFLDRWDFPTCCLLTSGRIAADMILKAAAAKIPVVVSRSIPTTTAYEIARL
                     RGITVVGRIESKDPILYTRPERIIR"
     gene            2315..2554
                     /locus_tag="P5313_16340"
     CDS             2315..2554
                     /locus_tag="P5313_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRY81985.1"
                     /translation="MFGRIGPMELILILVIALVIFGPKKLPEIGKAIGTAIKEFKKHT
                     SNVSESIEEAVNAPDEAPKIQDAAKGKEPDSKKKG"
     gene            2606..>3133
                     /gene="tatC"
                     /locus_tag="P5313_16345"
     CDS             2606..>3133
                     /gene="tatC"
                     /locus_tag="P5313_16345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRY81986.1"
                     /translation="MSDAPMPFLAHVAELRKRLLLAFAALAAATVASFSFTEALAEYL
                     MHPVAGLSFVYLSPPELFLAYVRIAVLAGLVISSPVILFQVWLFVRPGLLKRERRAIL
                     AGLFFGAVFFAAGAAFSFFVILPFTLRFFLQYATERIEAVFSFGEYVGFVTSLVLSFG
                     AAFELPVVTAILVGGR"
BASE COUNT          530 a         1090 c         1016 g          497 t
ORIGIN      
        1 cgacttctac gaccgggtgg cccacaagaa gggcgagctc tggcacatcg cggacggatc
       61 cgcctggatc gcccagcgct ggggcctcgg ggacgactac tgggaccaca aggacaacaa
      121 gctctggacc cggggcggct accccgtgca ccactccaac gagtccgcgg gccaggtcgg
      181 ggcgctcatc tcctgcttca cgaaccggga cgcccagaac catacccacc agaacttcat
      241 cggctcccaa ctgcccatcg cgatccagaa ggagatcgcc ggggagatct tcggtagccc
      301 ggatgccctg gatccgcccc agaattacac ccggatgaac gagttcaagg cccgcttcgt
      361 caagtgggcc ttggtccgcg gccggctcca cgactccctg accctgtgca actggatgtg
      421 gcccatggcg gtgagccccc tcaaggagcg gaagtaccgc ggcgacacct ccctggaggc
      481 ccagttcttc tccgcggtca cgggcgtaac caagaccgag gcggagctgg acaaggacgc
      541 cgagcgggtg gtgaccctct tccgggccct gaccgtccgg gccatgggca cggtggacat
      601 gcggggcaag cacgacgcca tgaacaagtg gatctacacc ccggacaaga aggacggcaa
      661 ggagataccc cagttcacca agggcacgat ccagatggag aaggacgacc tggataaggg
      721 actgggcatg gtgtacaagc agttcggctg ggacgagaag accggagccc cgaccaaggc
      781 gggcctggag gcgatcggcc tcaaggacgt cgccgacgag ctgggatcca agggcctgct
      841 gcccgcgtaa ccgcgattcg gcgggggtgt ccagtacgcg accggcgtat tggacacccc
      901 gggccgtttc cgcccgtcga cgaacgccgc cgattcctat atcctgaaat caaggatccc
      961 gatggaaacc tccgatgtaa cggcgagcga tgcgccgtcc gcggtcggaa ccgtccggcg
     1021 cgacagcgcc gcgggccggc tgaccctgct ttccgcactg cgcgccgatg ccgcgcgaaa
     1081 cgcctcgctc cctccggccg ctgccgagat cgggcaggac gccccctcgt ccgcggttcc
     1141 cgcggatccc ctggacgaga tcgggggttc cggggccgag gacctgaaga ggatggacgg
     1201 ccgggcggag acctatttct tcagcgacct gtacatgacc cgggcgtacg cgtcctacct
     1261 ctaccgcctg gcggagaagg atctcgtcgg gctggtcgtg gagacggtcc gggaggattc
     1321 ccggctgtat ccccggccca cccccctgga cttcttccgg ggtccgccct tcacgctcgc
     1381 ccaggatcag ctggacgata tcctgacccg gatccgggag cgcgaagact gccgggatat
     1441 ccgggaatgc cgggcttcca acggcgcccg ctacctctac tcgaccctgc acctgaccgt
     1501 cgcccacgcg gaggccctca cggaatggaa tgaagtgggc caggcggaga acccgtgagc
     1561 gacgcacgac ccgacctgcc cctggccccg gagtaccccg tggaattgac cgtgaacggc
     1621 acccggctgg ccaccttcct ctgcacaccg gagcacctgg acgacctggc cgcgggcaac
     1681 cttttcaccc ggggatccct ggtagacccg ggccagatcc ggaccctggg ggcctgtccc
     1741 gacctccgga tcgtggcggt gaccaccagg acccccctgg gggaggagag cctgggtctg
     1801 tcccgggtga tcgcctcggg ctgcggggca gggctggagt tctcggagga tttcaagaac
     1861 gccccggccc tgccggaggg attctccgtg gagctctcca gcctgcgtgc ccgggctcgg
     1921 gccatgttcg aggccgcggt ccagtaccgc cggaccgggg gcatgcactg cgccagcctc
     1981 gccggccggg acggccggac ctgcgtccgg gaggacgtgg gacggcacaa cgccgtggac
     2041 aaggtcatcg gccggggctt cctggatcgg tgggacttcc cgacctgctg tctcctgacc
     2101 agcggccgga tcgcagcgga catgatcctg aaggccgccg ccgcgaaaat ccccgtcgtg
     2161 gtctcccgga gcatcccgac caccacggcc tacgagatcg cccgtctacg gggcatcacg
     2221 gtcgtgggcc gtatcgaatc taaggaccct atactctaca cccgcccgga gcgtatcatc
     2281 cggtgaggga agcgcggaaa cgaggaggta tcgaatgttc ggacgcatcg gccctatgga
     2341 gctcatactc atcctggtca tcgccctggt gatcttcggg cccaagaagc tgccggagat
     2401 cggcaaggcc atcggcacgg ccatcaagga attcaagaag cacaccagca acgtgtcgga
     2461 gagcatcgag gaggcggtca acgcaccgga cgaggcgccc aagatccagg acgccgccaa
     2521 gggcaaggaa cccgattcca agaagaaagg gtaggggttt gcccggtcgg cggagcccct
     2581 ccgcccccga tccccggaga gcgctatgag cgacgcaccc atgcccttcc tggcccacgt
     2641 ggccgaactc cggaagcgcc tcctcctggc cttcgcggcc ctggcggccg cgacggtcgc
     2701 gagcttctcc ttcacggaag ccctcgcgga atacctcatg catcccgtgg cgggcctgtc
     2761 cttcgtctac ctctccccgc cggagctctt cctggcctac gtccgcatcg cggtcctggc
     2821 ggggctcgtg atctcctcgc cggtgatcct cttccaggtc tggcttttcg tacgccccgg
     2881 cctgctgaaa cgggagcgcc gggccatcct ggcgggcctc ttcttcgggg cggtcttctt
     2941 cgcggccgga gcggccttct cgttcttcgt gatcctgccc ttcaccctgc ggtttttcct
     3001 gcagtacgcc accgagagga tcgaggcggt cttctccttc ggggagtacg tcggcttcgt
     3061 gacctccctg gtcctgtcct tcggcgcggc cttcgagctt cccgtggtca ccgccatcct
     3121 ggtgggcggc cgg
//
LOCUS       DAOTEI010000262         3087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_24618_length_3087_cov_4.529683, whole genome shotgun sequence.
ACCESSION   DAOTEI010000262 DAOTEI010000000
VERSION     DAOTEI010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24618_length_3087_cov_4.529683"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2300)
                     /locus_tag="P5313_16350"
     CDS             complement(<1..2300)
                     /locus_tag="P5313_16350"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HRY81987.1"
                     /translation="MNYKELPVYQQKDRILDSLREHQVIVVESPTGSGKTTQIPVILH
                     EAGYARNGIIGVTQPRRIAAISVTDFISRQLGTPVPGLVGYKMRFEDKTDTSTLIKIM
                     TDGILLQEMKLDPLLSRYGVLMVDEAHERSLNIDFILGLLKRVMEARPEFKVVVSSAT
                     INAEVFSAYFGDCPVVKIETPMYPVTVVYDPPAAESDMDALIDKVESTIVRIVQEGRE
                     GDILVFLPGERTIKDCMQRLAACEVRRSLHLIPLYGRLGKDEQERVFEPTPAGKTKVV
                     VSTNIAETSVTIDGITSVIDSGLAKLNFYNPRTYTASLVEVPTSKASSNQRKGRAGRT
                     RPGSCYRLFGRDDFENRSLFTMEEIYRTDLSEVVLRMAELGITDFERFDFISKPAKTG
                     ILGAVEALNLLDALNPDRTLSPVGEKMCLFPLLPRHSRILVEAVLRYPDVLEEALIAT
                     AFLSTQGPFILPPGEELEARAAHHAFRDPAGDFVSYLKIFRQYKEARNPVRYCEKSYL
                     DERTMAEIVRVKEQLELIVADMGVPISGGGSVEDYLCAVSRGLIQFVCARQDRGTFRS
                     LTAERIQIHPGSVMFKENPQFIVAGEIVRTTRMYAMSVSPLQKHWLPKISPLVAEGLL
                     SHAAVKAVRGQAAKAVQEERAARSAARDFTNQIKIAGEVFRLEVGKKKKKLAVLDWEA
                     LKRVRGETARDSAELYKNLKGVVTRDNLRLLDGEKLDLILRVAPLLDLDRDLERDWPK
                     KRNFAVHEDSAALVSALDHVLQVSRW"
     gene            2414..>3087
                     /locus_tag="P5313_16355"
     CDS             2414..>3087
                     /locus_tag="P5313_16355"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRY81988.1"
                     /translation="MLREVFVDSLIFVCLFGIAVGRLPFLRMNRAALAFAGAAVLILA
                     GALTPRAAFEAVDLEALALILAMMVVAANLRISGFFSWASERALLLARSPRGLLAAVI
                     AVSGIFSALFLNDTMCLVLTPLVAELALSGRRDPIPYLIGVATAANVGSCATIIGNPQ
                     NMLIGASSGIPFGSFFLKLALPSAAGLAVCWIVIVLAFPKEFREGRLSSASAAVPPAP
                     ENPGLLR"
BASE COUNT          461 a         1004 c         1023 g          599 t
ORIGIN      
        1 ttccagcgtg acacctgcag gacgtggtcc agggcggaaa ccagagccgc ggagtcctcg
       61 tgcaccgcga agttacgttt tttcggccag tcccgctcca ggtcgcggtc caggtccagc
      121 aggggggcta cgcggaggat caggtcgagc ttctccccgt ccaggagccg caggttgtcc
      181 cgggtgacga ctcccttcag gttcttgtag agttccgcgg aatcccgggc cgtctctccc
      241 cggacgcgtt tcagagcctc ccagtccaac accgcaagct tcttcttttt cttgccgacc
      301 tccagcctga agacctctcc ggcgatcttg atctggttgg tgaagtcccg ggccgcggag
      361 cgggctgcgc gttcctcctg cacggccttc gccgcctgtc cccgcaccgc cttgaccgcg
      421 gcgtgggaca aaagaccctc ggccaccagg ggcgatatct tgggcagcca gtgcttctgc
      481 aggggggaga cgctcatggc gtacatgcgg gtcgtccgga cgatctcccc ggccacgatg
      541 aactggggat tctccttgaa catcacggaa ccggggtgga tctggatccg ctcggcggtg
      601 aggcttcgga aggttccccg gtcctgccgg gcgcagacga actggatgag cccccggctc
      661 acggcgcaga ggtagtcctc gacgctgccc cccccggaga tgggcacccc catgtccgcg
      721 acgatgagct ccagctgttc cttgacccgt acgatctccg ccatggtccg ctcgtccagg
      781 tagctcttct cgcagtaccg gaccgggttc cgggcctcct tgtactgccg gaagatcttg
      841 aggtacgaga cgaagtcccc ggcgggatcc cggaaggcgt ggtgtgcggc ccgggcctcc
      901 agctcctccc cggggggcag gatgaagggc ccctgggtgg acaggaaggc cgtggcgatc
      961 aaggcctcct ccaggacatc cggataccgc aggacggctt ccaccaggat gcgggagtgc
     1021 cggggcagga gggggaagag gcacatcttc tcgcccaccg gggaaagggt acggtcgggg
     1081 ttcagagcgt ccaggaggtt gagggcttcc acggcgccca ggatgccggt cttggcgggc
     1141 ttggagatga agtcgaaacg ctcgaagtcc gtgatcccca gttcggccat gcgcaggacc
     1201 acctccgaga ggtcggtccg gtagatttcc tccatggtga agagggaccg gttctcgaaa
     1261 tcgtcccggc cgaacaggcg gtagcaggac ccgggccggg tgcggcccgc cctccccttc
     1321 cgctggttgc tggaggcctt ggacgtgggg acctccacca gactggcggt ataggtgcgg
     1381 ggattgtaga agttcagctt ggcaaggccg gagtcgatta cgctggtgat gccgtcgatg
     1441 gtgaccgagg tctccgcgat gttcgtggag accaccacct tggtcttccc ggccggggtg
     1501 ggctcgaaga cccgctcctg ctcgtccttt cccaggcggc cgtacagggg tatcaggtgc
     1561 aggcttcgcc ggacctcgca ggcggccagc ctctgcatgc agtccttgat ggtgcgctcg
     1621 ccggggagga acaccaggat gtccccttcc ctgccctcct ggacgatcct gacgatcgtg
     1681 gattccacct tgtcgatcag ggcgtccatg tcggactccg cggcgggcgg atcgtacacc
     1741 accgtgacgg ggtacatggg cgtctcgatc ttgaccacgg ggcagtcccc gaaatacgcg
     1801 ctgaacacct cggcgttgat cgtggccgag gagaccacca ccttgaactc cggccgagcc
     1861 tccatgaccc gcttcaggag gcccaggatg aaatcgatgt tgaggctccg ctcgtgggcc
     1921 tcgtcgacca tgagcacccc gtaccggctg agcaggggat ccagcttcat ctcctggagg
     1981 aggatcccgt cggtcatgat cttgatgagg gtggaggtgt cggtcttgtc ctcgaagcgc
     2041 atcttgtagc ccaccaggcc gggaaccggg gttcccagct gacgggagat gaaatcggta
     2101 accgagatgg cagcgatccg gcggggctgg gtcacgccga tgatcccgtt ccgggcgtag
     2161 ccggcctcgt gaaggatgac gggaatctgg gtggtcttgc cggagcccgt ggggctctcc
     2221 accacgatca cctggtgttc ccggagggag tcgaggatgc ggtccttctg ctgatagacg
     2281 gggagttcct tgtaattcat gcttaccatt gtagagcttt cccctccgtt atcctaggga
     2341 gtacggaccc gggacgttgc ggtgggcccg gaccctcggt ataatccgtg cggccgccgc
     2401 ggccggagga agcatgcttc gcgaggtctt cgtggattcc ctcatcttcg tctgtctctt
     2461 cgggatcgcc gtgggacggc tccctttcct tcggatgaac cgtgcggccc tggccttcgc
     2521 gggggcggcc gtcctcatcc tcgcgggggc cctcacgccc cgggcggcct tcgaggcggt
     2581 ggatctggaa gccctggccc tgatcctggc catgatggtg gtagcggcga acctccggat
     2641 ctcggggttt ttctcctggg cttccgagcg agccctcctg ctggcccggt cgccccgggg
     2701 ccttctggcc gcggtgatcg cggtatccgg gatcttcagt gccctcttcc tgaacgacac
     2761 catgtgcctc gtgctcaccc ccctggtcgc cgagctcgcg ctctccggcc ggcgggatcc
     2821 gatcccctac ctgatcggcg tggcgaccgc ggcgaacgtc ggttcctgtg ccaccatcat
     2881 agggaatccc cagaacatgc tgatcggggc gtccagcggg atccccttcg ggtccttctt
     2941 cctgaagctg gccctgccgt ccgccgcggg actcgcggtc tgctggatcg tcatcgtcct
     3001 cgcgttcccg aaggaattcc gcgaaggccg tctttcctcc gcgagcgcgg cggttccacc
     3061 ggctccggag aaccctggcc tactccg
//
LOCUS       DAOTEI010000263         3060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_24942_length_3060_cov_5.981697, whole genome shotgun sequence.
ACCESSION   DAOTEI010000263 DAOTEI010000000
VERSION     DAOTEI010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24942_length_3060_cov_5.981697"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="P5313_16360"
     CDS             complement(<1..643)
                     /locus_tag="P5313_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRY81989.1"
                     /translation="MKVAINGFGRIGRLVFQSIVDQGLLGKGKIDVVAVVDISTDAEY
                     FAYQLKYDSVQGRMKADISAKKSDPSLAEADVLVVNGHEIKCVMAEKELKNLPWAKLG
                     VEYVIESTGLFTDEKAYGHLDAGAKKVIVSAPAKSKSEEKPIKTFVIGVNEKDYDAAK
                     HHVVSNASCTTNCLAPVVHVLLKEGIGIETGLMTTIHSYTATQKTVDGPSKKDW"
     gene            complement(878..2800)
                     /locus_tag="P5313_16365"
     CDS             complement(878..2800)
                     /locus_tag="P5313_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRY81990.1"
                     /translation="MPLLECRNVGKDFGGEPVLTGVSLSLEPGSKTALVGRNGTGKTT
                     LVRILLGLDEDFRGTVIRAPRIRVGYVPQHVEPPEGVSALEWLLNEVKEERSRLVRLE
                     HEMADGSGAALERLLAEYGALREAYEERNGDLAEDQARRLLSRAGLAGTADREAARLS
                     GGERNVLALVKALMENPDLLVLDEPGNHLDFWGLSWLEDFLRGLPKAVLLVSHNRWLI
                     DRVADRVLELEGGKLAEYAGGYSAYRMEKLRRAAGQGADWQADRKRIERLEELVRRFE
                     QIARARPDPAWGKRLRARRSQLAREKEAATQKPVLDGRRIELDFRDADSRSDIALDVR
                     GYSKSFGDRALFDAASLDVLTGERVALVGRNGSGKTTFLKDLVEHGNWDHGILRIGPS
                     MRVGYCAQDRTVFRPERTVVQEFEALDAKREETWKLLKRFLFRPEDLDRRIDTLSGGE
                     LNRLQLARAVYLKANLLILDEPTNHMDIPGCEAVEDGLAEYPGTILAVSHDRYFLEKV
                     ADRVVLLEDGRFVPYEGTFSEFWRDLGSTRAGAARGLAGRGQSLKIAERGQSSKKGDA
                     TVEEGPGTRSKGSKRPEGGQSSVEDRIQALEAEKAVLEARAAELFQTRDFKGSSRVTL
                     DLEKLNRRIEKLYGEL"
BASE COUNT          488 a         1036 c          946 g          590 t
ORIGIN      
        1 gccagtcctt cttggacggg ccgtcgacgg tcttctgggt ggcggtgtag gagtggatgg
       61 tggtcatgag gccggtctcg atgccgatgc cttccttgag caggacgtgg acgaccgggg
      121 ccaggcagtt ggtggtgcag gaggcgttgg agacgacgtg gtgcttggcc gcgtcgtagt
      181 ccttctcgtt caccccgatg acgaaggtct tgatgggctt ctcctcgctt ttgctcttgg
      241 cgggggcgga gacgatgacc ttcttggcgc cagcgtccag gtggccgtag gccttctcgt
      301 ccgtgaagag cccggtggac tcgatcacgt actcgacccc cagcttggcc caggggaggt
      361 tcttgagctc cttctcggcc atcacgcact tgatctcgtg gccgttcacc acgaggacgt
      421 ccgcctcggc cagggaggga tcgctcttct tggcggagat gtccgccttc atccggccct
      481 gcacggaatc gtacttgagc tggtaggcga agtactcggc gtccgtggag atatccacca
      541 cggcgacgac gtcgatcttg cccttgccga gcaggccctg gtccacgatg gactggaaca
      601 cgagtcttcc gatgcgcccg aatccgttga tcgcaacctt catgattcct cctaggggga
      661 ctatctataa ttccgtatag aaacgtatcg aataggactt ttagcgtcaa tcaggccgta
      721 ccgaaatccc ttccaagtat ccacaaaaag tgataaattt accatggatt cagccgataa
      781 aaagagtgtc ggaggcattc catagttccc ctcccagttt actatggagt gtcccggcac
      841 cttcacaggg cgggccggtc gaaagaccgg ccttttttta cagttccccg tacagcttct
      901 ctatcctccg gttcagcttc tccaggtcca gggtaacgcg gctggagccc ttgaagtccc
      961 gggtctggaa gagctccgcg gctcgggcct ccaggaccgc cttttccgcc tcgagggctt
     1021 ggatgcggtc ctccacggag ctctgtcccc cttccggccg tttcgagccc ttcgaccgcg
     1081 tcccggggcc ttcctccaca gtcgcatctc ccttttttga gctctgtccc cgttccgcga
     1141 tcttcaagct ctgtccccgg cccgcaaggc cccgagcggc gccggcccgg gtcgaaccca
     1201 ggtccctcca gaactcggag aaggtgcctt cgtacggcac gaaccggccg tcctccagca
     1261 ggaccacccg gtccgcgacc ttctccagga aataccggtc gtgggaaacc gctaaaatgg
     1321 ttccggggta ctcggcaagc ccgtcctcca cagcctcgca gcccgggatg tccatgtggt
     1381 tcgtgggctc gtccaggatg aggaggttgg ccttgaggta gacagcccgg gccagctgga
     1441 gccggttgag ctcccctccg gagagggtgt cgatccggcg gtccaggtcc tcggggcgga
     1501 agaggaaccg cttgaggagc ttccaggtct cctcccgctt ggcgtccagg gcctcgaact
     1561 cctgaaccac cgtgcgctcg gggcggaaga ccgtgcggtc ctgggcgcag taccccaccc
     1621 gcatgctcgg gccgatccgg agaattccgt gatcccagtt cccgtgctcg acgagatcct
     1681 tgaggaaggt cgtctttccg gacccgttcc gccctaccag ggcgacccgc tccccggtca
     1741 aaacatccag ggacgcggcg tcgaagagag ctctgtcccc gaaggacttc gagtatcctc
     1801 gcacgtccag ggcgatgtcc gagcgggaat ccgcgtcccg gaagtcgagc tcgatgcggc
     1861 gcccgtccag cacgggtttt tgcgtggccg cttctttctc ccgggccagc tggctgcggc
     1921 gggccctaag gcgcttcccc caggcgggat cggggcgggc ccgggctatc tgctcgaagc
     1981 ggcggaccag ctcttccagg cgctcgatcc gcttccggtc cgcctgccag tccgcgccct
     2041 gcccggcggc acgccgcagt ttctccatgc gataggcgga atacccgccc gcgtattccg
     2101 cgagcttccc gccctccagc tccagtacgc gatccgcgac ccggtcgatc agccaccggt
     2161 tatgggagac caggagcacc gccttgggca ggccccggag gaagtcctcg agccaggaaa
     2221 gcccccagaa gtccaggtgg ttcccgggct cgtccagcac caggaggtcg ggattctcca
     2281 tcagggcctt gaccagggcc aggacgtttc gctccccccc ggagagccgg gcggcctccc
     2341 ggtccgcggt tcccgccagg cccgcccggg aaagcaggcg ccgggcctgg tcctccgcta
     2401 ggtctccgtt cctctcctcg taggcttccc ggagagcccc gtattccgcc aagagccgct
     2461 ccagggcggc cccggacccg tcggccattt cgtgttccag gcgtacgagg cgggagcgct
     2521 cctctttgac ctcattcagg agccattcca gggccgatac gccctcgggg ggttcgacgt
     2581 gctgggggac atagccgacc cggatccggg gggcccggat cacggttccc cggaaatcct
     2641 cgtccaagcc caggaggatg cgcaccaggg tggtcttgcc ggtcccgttg cgtcccacca
     2701 gggcggtctt ggagcccggc tccaaggaca gggacacccc ggtgagcacc ggttcccctc
     2761 cgaaatcctt tcccacgttc ctgcattcca acagcggcat cacgactcct tttctcgggt
     2821 gtccaaaaaa ccgactgtgc tttttggaca gccccggcat cccggcccga tccgggcagg
     2881 gtaggtgcgg cccctgcggt tcctcgcctc cggctcagga gccggaggga cgaccctcga
     2941 accgaggggg acggccgcgc gcctatccgg aaatgccgga aaaaaaaagc gcggccgtcc
     3001 gtaggcggcc gcgcggtgcg ttcgattgaa gcttttctca aatatctcca accgatcctg
//
LOCUS       DAOTEI010000264         3054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_25017_length_3054_cov_4.578860, whole genome shotgun sequence.
ACCESSION   DAOTEI010000264 DAOTEI010000000
VERSION     DAOTEI010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25017_length_3054_cov_4.578860"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2049)
                     /locus_tag="P5313_16370"
     CDS             complement(<1..2049)
                     /locus_tag="P5313_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81991.1"
                     /translation="MRFRAVPFLILALSAAHGPAQDGNLAWMPPPGRYSSARVSPALP
                     PGTSGEYRFLENGDGIWRPLEGPLELTAFPREERSYVLEVRGSKGTERFEGRARYVID
                     RFPPPAPVVSPAPGSYEGRPEIRLSSEPDAQIYYSLEALDRPSPGFRPYDSDRPPVLD
                     RPRDGTRAWTLSAYAVDRAGNPGPISTARYVVEPSPTGPRPGDSGKAAESALGTGSDL
                     EYRVERKSPGTAIVYFPSPEGGRILAAVNPRDPSDPRYYAELPAGGREALLEVSAPAG
                     WIGPLLIRYARWADGKPALAPGGVEIRFAFEDPGKPPPAPAEPVVLYPPGTRTVLLSW
                     EPSPFRIEVAVGPGPFTPYSLPISVPVPEDSRGVEIRYRAVGPSGGSSEVRTLALAPS
                     TQPILPEISGLPTTVRTNEAVRPRAAPGTFIRYEASVEGFPPAVTTASPLLDEGVSFS
                     GEPGKDVRYYLRFRAFSDASPEAQGSDERFAEFLIDRSPPPAPRLAAGSLDGDTEEDR
                     IVAFEPGEGTIRFAAVEKGSPGDPDFRDYSGPVTLEGSGDRPRAYDVYAYAVDEAGNR
                     SETLGPVSVRIDRASVYVAPWGRDSDGGGPRNPLATVSAGVAAASKTGRRFVRVMGDV
                     RLGGPVRVGTEVEILGGCDESWEPVPGRRSGVSAEGFSGPLFIVQGSALVLRNLTIEA
                     D"
     gene            complement(2051..2656)
                     /locus_tag="P5313_16375"
     CDS             complement(2051..2656)
                     /locus_tag="P5313_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HRY81992.1"
                     /translation="MRKEFLPYDTVRNNALKLAHRIHSDGFVPDIIYVSLRGGAYLGN
                     VISEYFKIVRKSDRPVFYAAVVAHSYTDVQAHEQVRIDGWTYSPEYLRHGDRVLLVDD
                     IYDSGRTINHLVEILLEKGIPRTDIKVAVHDYKAAKYRKEELPIRPDYYCRKIEVDSP
                     ENEVWIHYMSHELVGLSEEELQTHYYPEDPELKDILKVLKK"
     gene            complement(2777..>3054)
                     /locus_tag="P5313_16380"
     CDS             complement(2777..>3054)
                     /locus_tag="P5313_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRY81993.1"
                     /translation="GLEVVSLEAEGIPEGRVPKGTSGVYLSNVIARTPADTIGMKPGD
                     IVQDVNERKVRNAAEFYKALNEPKDSKIQFNFLREGQTLSSLAYIRK"
BASE COUNT          473 a         1023 c         1066 g          492 t
ORIGIN      
        1 gtccgcctcg atggtcaggt tccgcaggac cagagcgctg ccctgtacga tgaagagggg
       61 tccggagaat ccctcggccg agaccccgct gcgccgtccc ggcacgggtt cccaggactc
      121 gtcgcagccg cccaggatct cgacctccgt cccgacccgg accgggcctc ccagacggac
      181 atcccccatg acccggacga agcgccggcc ggtcttcgac gccgcggcca cccccgcgga
      241 aaccgtggcc aggggattcc ggggcccgcc cccgtccgaa tccctgcccc agggggccac
      301 atacaccgaa gcccggtcga tgcgcacgga cacgggcccc agggtctccg atcgattccc
      361 cgcctcgtcc accgcgtagg cgtacacgtc gtaggcccgg ggccggtccc cgcttccctc
      421 cagggtcacc ggtccggagt agtcccggaa gtcggggtcc ccggggctcc ccttctccac
      481 cgcggcgaac cggatggttc cctccccggg ctcgaaggcc acgatccgat cctcctcggt
      541 gtccccgtcc aggcttccgg cggccagacg cggcgcggga gggggcgacc ggtcgatcag
      601 gaattcggcg aaccgctcgt cggatccctg ggcctcgggg gaggcgtccg agaaggcccg
      661 gaaccgcagg tagtaccgga catcctttcc cggctccccg gagaaggaga ccccctcgtc
      721 caggaggggg gaggctgtgg tcacggcggg ggggaacccc tcgacggatg cctcgtaccg
      781 gatgaaggtg cccggggcgg cccgcggccg gaccgcttcg ttcgtgcgga ccgtcgtcgg
      841 caggcccgag atttccggca ggatcggctg ggtcgaggga gccagggcca gggttcgaac
      901 ctcggaactg cccccggaag gccccacggc ccggtaccga atctccacgc cccgggagtc
      961 ctcgggtacg ggcacggaga tcgggagaga atacggggtg aagggtccgg gtccgacggc
     1021 cacttcgatc cggaaggggg acggttccca ggacaagagg accgtgcggg tgccgggcgg
     1081 atacaggacg accggttcgg cgggagccgg cgggggcttt ccgggatcct cgaaggcgaa
     1141 gcggatctcc acccctcccg gggcaagggc gggctttccg tccgcccacc gggcgtaccg
     1201 gatgaggagg gggccgatcc aaccggcggg cgcggagact tccaggaggg cttcccggcc
     1261 cccggcgggg agctcggcat agtatctcgg gtccgagggg tcccggggat tcaccgccgc
     1321 caggatcctg ccgccctccg gcgagggaaa gtagacgatc gcggtccccg gggatttgcg
     1381 ctccacccgg tattccaggt ccgatccggt tcccagggcg gattccgcgg ctttcccgga
     1441 atcgcccggc ctaggtccgg tgggcgacgg ttccacgacg taccgggccg tgcttatggg
     1501 accgggattt ccggccctgt ccaccgcgta cgccgacaac gtccacgcgc gggttccgtc
     1561 ccggggacgg tccaggacgg gcggacggtc ggagtcgtag ggccggaatc ccggggacgg
     1621 ccggtccagg gcttccaggg agtagtagat ctgggcatcc ggctccgagg agagccggat
     1681 ctccggcctt ccctcatagg agccgggagc gggcgagacc acgggagcgg gcggggggaa
     1741 gcggtcgatc acgtaccggg cccggccctc gaaccgctcg gtccccttcg agcctcgaac
     1801 ctccaggacg taggagcgct cctcccgcgg gaaggccgtc agttccaggg gtccctcgag
     1861 ggggcgccat atcccgtccc cgttctccag gaaccggtac tccccggacg tccccggcgg
     1921 gagcgcgggg ctgacccggg cggaggaata ccttcccggc gggggcatcc acgcgaggtt
     1981 cccgtcctgg gcgggcccgt gcgccgccga tagcgcgagg atcaggaacg gaacggctcg
     2041 gaagcgcatg ctacttcttg aggaccttga gtatgtcctt gagctccgga tcctcgggat
     2101 agtagtgggt ctgcagttcc tcctcggaaa ggccaaccag ttcgtggctc atgtagtgga
     2161 tccagacctc gttctccggg gaatccacct cgatcttgcg gcagtagtag tccggccgga
     2221 tggggagctc ctccttgcgg tacttggcgg ccttgtagtc gtggaccgcg accttgatgt
     2281 ccgtccgggg tatccccttc tccaagagga tctccacgag gtggttgatc gtgcggccgg
     2341 aatcgtagat gtcgtccacc aggaggaccc ggtccccgtg gcggaggtac tcgggactgt
     2401 aggtccagcc gtcgatacgg acctgctcgt gggcctggac gtccgtgtac gaatgcgcga
     2461 cgacggcggc gtagaacacc ggccggtcgg acttgcggac gatcttgaag tattcggaga
     2521 tcacgtttcc gaggtacgcc ccgccccgga gggaaacgta gatgatgtcc gggacgaatc
     2581 cgtccgaatg gatgcggtgc gccagtttga gagcgttgtt tcggaccgta tcgtagggca
     2641 ggaattcctt gcgcatgggc cgtcctttct cggatcgatg gctcggatcc gtccgatcct
     2701 ccgcctggct ccggaattcc cgcggcctta aaaggaaagg ccgccccgga agtccgggac
     2761 ggccccaatt ctagccttac ttgcgaatgt aggcaaggga agacagggtc tgtccctccc
     2821 gaaggaagtt gaactggatc ttggaatcct tgggctcgtt cagggccttg tagaactccg
     2881 ccgcgttgcg caccttcctc tcgttgacat cctgcacgat atccccgggc ttcattccga
     2941 tggtgtccgc cggggtccgg gcgatcacgt tggacaggta cacccccgag gtccccttgg
     3001 gcacccggcc ctcggggatg ccctcggctt ccagggagac gacctcgaga cccg
//
LOCUS       DAOTEI010000265         3016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_25493_length_3016_cov_4.768997, whole genome shotgun sequence.
ACCESSION   DAOTEI010000265 DAOTEI010000000
VERSION     DAOTEI010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25493_length_3016_cov_4.768997"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="P5313_16385"
     CDS             <1..139
                     /locus_tag="P5313_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY81994.1"
                     /translation="GADSLDTYELVYGIEEELGITIPDEKANEFETVKDAYEFIKSQI
                     K"
     gene            157..915
                     /gene="rnc"
                     /locus_tag="P5313_16390"
     CDS             157..915
                     /gene="rnc"
                     /locus_tag="P5313_16390"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRY81995.1"
                     /translation="MFGTSGLSRERRDQLAAFQKKIGVRFKSVELLSRALTHRSRTNE
                     DPSRNENNERLEFLGDAVLGMVAASYLFRKLPERAEGELARIKSFIVSEDTLSEVALD
                     LGVDRALLIGKGEEQSGGRTKKAILADAMEAVFGACYLDQGFDKACSFILKLLAPEVD
                     KVLANRHRKDYKTIVQEFVQKQYRSYPKYTVVRRSGPDHDRTFWIACVIEDREYGPAE
                     GKNKKEAEQKAASLAYEAIAAAGGPDAQLLRELG"
     gene            957..2144
                     /locus_tag="P5313_16395"
     CDS             957..2144
                     /locus_tag="P5313_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81996.1"
                     /translation="MKTRIPRLAVLAAVVLAFAGAPPAPAQTAPKLSVAVLGLENSGG
                     DPRQDYLASLAEGILRYDLSVLADIPLVERRNLDKLLSEKEFSLSAAADPARAARAGG
                     LIGASHLILGEYAVVAGELSLTVHLVDAASGQAVTFRDRGASEHAVHRIAEKIAARLL
                     DRRGIVFADAANDRSILSLRDETPGIIALHSPIRSAEIYLDGEFVGFTKGNEIEPILL
                     ESVRPGRHTLRVHLSGGDFGVVILPQVEFKDWETVVDVRPGGRHAVRDQTRHFNETLY
                     RLEYLGSFELKAERGKGSALGFSRELSFTDRTGKTVPVKLTGTPSETPEGVAVSLTFT
                     VGGQEARGRIAFPARPDAPAELRITAGSAALEVELSDSWGSWELDVTVRRTDVYQGLH
                     REK"
     gene            complement(2286..>3016)
                     /locus_tag="P5313_16400"
     CDS             complement(2286..>3016)
                     /locus_tag="P5313_16400"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY81997.1"
                     /translation="SPRPSYGLKLLEDTGMLPDILPELSACRGVEQKGFHRFDVLDHS
                     FLSCDGAPDRIELRLAALLHDIGKPAARVEGPDGLPTFHRHEELSARGTEEALRRLKF
                     PNAVIASVSHLVRHHMFAYDDSWGDAAVRRFLARTGPENLEDLLALRLADAYGTTGER
                     PDPRGLDPLRRRVEDLLGRSRALSIKDLAIGGNELAALGVPRGPVMGRILAELLETVL
                     DDPELNTSERLLDIARRIKEKYGV"
BASE COUNT          513 a         1023 c          991 g          489 t
ORIGIN      
        1 cggcgccgac agcctcgaca cctacgagct ggtctacggc atcgaggaag agctcggcat
       61 cacgatcccg gacgagaagg ccaacgaatt cgagaccgtc aaggacgcct acgagttcat
      121 caagtcccag ataaagtaag ttcccttcgc gccgtgttgt tcggaacgag cgggctgtcc
      181 cgcgagcgtc gcgaccaact cgcggcattc cagaagaaaa tcggggtccg gttcaaatcc
      241 gtcgagctct tgagccgggc cctgacccac cggtcccgca ccaacgagga cccctcccga
      301 aacgagaaca acgagcgact cgagttcctg ggcgacgccg tcctgggcat ggtcgcggcc
      361 tcctacctgt tccgcaagct ccccgaacgc gcggagggcg aacttgcccg catcaagagc
      421 ttcatcgtct ccgaggacac cctgtccgag gtcgccctgg acctgggggt ggaccgggcc
      481 ctcctgatcg gcaaggggga ggagcagtcc gggggccgga ccaagaaagc catcctggcg
      541 gacgccatgg aggcggtctt cggggcctgt tacctggacc agggtttcga caaggcctgc
      601 tccttcatcc tcaagctttt agccccggag gtggacaagg tcctcgccaa ccggcaccgg
      661 aaggattaca agaccatcgt ccaggaattc gtccagaagc agtaccggtc ctaccccaag
      721 tacaccgtgg tgcgcaggtc cgggccggac cacgaccgga ctttctggat agcctgcgtc
      781 atcgaggacc gggagtacgg ccccgcggaa ggcaagaaca agaaggaagc ggagcagaag
      841 gccgcttccc tggcctacga ggccatcgcg gctgcggggg gaccggacgc ccagctccta
      901 cgggagctgg gctgaaaccc ttgcccccgg ccgtcgcccg tcgtacaatc cccaccatga
      961 agacccgaat tcctcgcctc gccgtcctgg ccgccgtcgt cctggccttc gcgggagctc
     1021 cgcccgcccc ggctcaaacc gccccgaagc tttccgtggc cgtcctgggc ctcgagaact
     1081 ccgggggaga tccccggcag gactacctgg cctccctggc ggagggcatc ctccgatacg
     1141 acctctcggt tttggccgac atccccctgg tggagcgcag gaacctggac aagctcctct
     1201 cggaaaagga attctccttg agcgccgcgg cggatcccgc ccgagcggcc cgcgcggggg
     1261 gcctcatcgg cgcgagccac ctgatcctgg gggagtacgc ggtcgtggcg ggggaactgt
     1321 cccttacggt ccacctggtg gacgcggcct cgggccaggc ggtcaccttt cgcgaccggg
     1381 gggcgtcgga gcacgcggtg caccgaatcg cggagaagat cgccgcccgg ctcctggacc
     1441 ggcggggcat cgtcttcgcg gacgcggcca acgaccggtc catcctctcc ctccgggacg
     1501 agactcccgg gatcatcgcc ctccattccc ctatacgctc cgcggagatc tacctggacg
     1561 gggagttcgt gggcttcacc aaggggaacg aaatcgaacc catcctcctg gagtccgtgc
     1621 gccccggtcg ccataccctc cgggtccacc tgtccggcgg ggacttcggg gtcgtgatcc
     1681 ttccccaggt ggagttcaag gactgggaga ccgtggtgga cgtgcggccg ggcggacggc
     1741 atgcggtccg ggaccagacc cgccacttca acgagaccct gtaccgtctc gagtacctgg
     1801 gctccttcga gctgaaggcg gagcgcggca agggttccgc cctggggttc tcccgggagc
     1861 tgtccttcac ggaccgaacc ggaaaaaccg ttcccgtcaa actgacggga accccctcgg
     1921 aaaccccgga gggggtggcc gtatcgctga cgttcaccgt gggaggtcag gaggcccggg
     1981 gacggatcgc cttccccgcc cggccggacg cccccgcgga gctccgcatc acggccggtt
     2041 cggccgccct ggaggtcgaa ctctcggact cctggggctc ctgggagctc gacgtgacgg
     2101 tccgccgcac cgacgtctac cagggcctgc atcgggagaa ataaagagct ttcacgcgag
     2161 gaataacagg atggacagga tttttttggg atcaacagga tgaagtctga aggtcaagat
     2221 tccaaactct tgattgatct tgtccatccg ttttccatcc tgttcatcct gtctcgcatt
     2281 gttcattaaa cgccgtattt ttcttttatc cttcttgcga tgtccaatag gcgctcggag
     2341 gtgttcagct ccgggtcgtc caggaccgtc tccagcaatt ccgccaggat cctgcccatg
     2401 accggccccc ggggcacccc cagggccgcg agttcgttcc cgccgatcgc caggtccttg
     2461 atcgacaggg cccgggaccg ccccaggagg tcctccacgc gccgtctcag ggggtccagc
     2521 ccccgcgggt cgggccgctc ccccgtcgtg ccgtaggcgt ccgccagccg gagggccagg
     2581 aggtcctcca ggttttccgg gcccgtccgg gccaggaagc ggcggaccgc ggcgtctccc
     2641 caggagtcgt cgtaggcgaa catgtggtgc cgcaccaggt gggagacgga cgcgatgacc
     2701 gcgttgggga acttgagccg ccgcagggct tcctcggtcc cgcgggcgga cagctcctcg
     2761 tggcggtgga aggtgggaag gccgtcgggt ccctccaccc gggcggcggg cttgccgatg
     2821 tcgtggagca gggccgccag ccggagctcg atccggtccg gggccccgtc gcaggacagg
     2881 aaggaatggt ccaggacgtc gaagcggtgg aagcccttct gctcgacccc ccggcaggcg
     2941 gagagctccg gcaggatgtc cgggagcatc cccgtgtcct ccaggagctt gaggccgtag
     3001 gagggccggg gggaca
//
LOCUS       DAOTEI010000266         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_25536_length_3012_cov_5.119378, whole genome shotgun sequence.
ACCESSION   DAOTEI010000266 DAOTEI010000000
VERSION     DAOTEI010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25536_length_3012_cov_5.119378"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="P5313_16405"
     CDS             complement(<1..318)
                     /locus_tag="P5313_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY81998.1"
                     /translation="MAILDYKGRRILGLLSFVLAAFPSPAPAEPPGGGSAPGPTAALR
                     AEASSRDGFSSALDLSIGAARLALRLSSAPGESPEARIGTTGPFWTAGPVRHSGLARV
                     LADP"
     gene            464..1570
                     /locus_tag="P5313_16410"
     CDS             464..1570
                     /locus_tag="P5313_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY81999.1"
                     /translation="MKGSDVSIEGVSKSFGTFTALRDVSMVIRKGEFYSLLGPSGCGK
                     TTLLRIIAGFDDPDGGEVSLDGTSVLNLPPNERHVNTVFQNYALFPHLTVFENVAFPL
                     RLKKTPNATVRSRVGDYLELVQLSSHAEKMPAQLSGGQKQRVAVARALINEPSVLLLD
                     EPLSALDAKLRQHMLIELDAIHDKIGITFIYVTHDQQEALSVSDRIAVMNAGQVLQIG
                     TPHEIYESPATGFVAAFIGETNMFEGTVESVEGTMVAVDVTGLGRILVTEDEPPAPGS
                     KASVVVRPEKIRIDTERPAPAKRTDINFLEGSVDEPIYSGSSTKYYVRHQSGTMLKVI
                     KQHANWSDEGPEIEWKDKVYLSWSADDAYLLGAS"
     gene            1579..2487
                     /locus_tag="P5313_16415"
     CDS             1579..2487
                     /locus_tag="P5313_16415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY82000.1"
                     /translation="MAVRSRTSRGPGRVRNYGPAYAGPAGIWLTVFFVAPLLVIVAYS
                     FMAKGLYGGVEPRFSLEAYRSLANSVFLRVSMNTLGISLVTTVLAILIALPCGYYMAR
                     SNRKTLLLMLVIIPFWTNFLIRIYAWIAILGNNGFLNALLLRAGIVAAPVQFLYNQVA
                     VTIVLVYTYLPYAILPLYSTIEKFDFSLLEAARDLGCTRRGAIFRVLLPNIRAGIITA
                     VLFVFIPTFGAYAVPLLVGGKESYMLGNFVAEELTRTRNWPLASAISMVITLVTTLGL
                     WLMFRLQGKAPQAKDPARSGGERRAP"
     gene            2535..>3012
                     /locus_tag="P5313_16420"
     CDS             2535..>3012
                     /locus_tag="P5313_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY82001.1"
                     /translation="MGFLFLPLVVLIVYSFNSAKSMSWGGFSLVWYERLFLKERNVWK
                     AFENSLIIAVSSAAVATCLGTLGAIGVNWYKFRFKKYVQAVAFLPLIMPEIIIGVSLL
                     IFFAGIQMKLGLLTIFIAHTSFNLPFVFLMVLARLDEFDYSIIEASRDLGATERQTL"
BASE COUNT          517 a         1043 c          913 g          539 t
ORIGIN      
        1 ggggtcggcc aggacccggg ccagacccga gtgccggacc ggtcccgctg tccagaaggg
       61 cccggtcgtg ccgatccgag cctccgggga ttccccgggc gccgacgaaa gccggagggc
      121 aagtcgggcg gcaccgatgg acagatccag ggccgaggaa aacccgtccc gggatgaggc
      181 ttcggcgcgc agggcggcgg tcggaccagg ggcggacccg ccgcccggag gttccgcggg
      241 ggcgggactc gggaaagcgg ccaggacgaa ggacaggagg ccgaggatcc ggcgcccttt
      301 gtaatcgagg atggccatga ttgccgcacc gtccgggaaa cccgaaggcg cttgcggttt
      361 ccgaaaaaaa gtttgacccg ggatgttgca tttttcgccc cccttcgcca ttattccccc
      421 agttccatct cggccggcca ccatcggagg gaaacgaaac gaattgaagg gcagcgacgt
      481 ctcgatcgaa ggcgtctcca agagcttcgg tacctttacc gcgctccggg acgtgagcat
      541 ggtcatccgg aagggcgagt tctactcgct cttgggtcct tcgggttgcg gaaagacgac
      601 gctcctccgc atcatagccg gcttcgacga tcccgacggg ggcgaggtgt ccttggacgg
      661 cacttccgtg ctcaacctcc cgccgaacga acgccacgtg aacaccgtat tccagaacta
      721 cgccctcttc ccccatctca cggtattcga gaacgtggcc tttccccttc gcctcaagaa
      781 gacgcccaac gcgacggtac gatcccgggt cggggactac ctggagctgg tccagctaag
      841 ttcccacgcg gagaagatgc ccgcccagct ctcggggggt cagaaacaac gggtggccgt
      901 ggcccgggcc ctgatcaacg aacccagcgt cctcctcctg gacgagccct tgtcggccct
      961 ggacgccaag ctccgccagc acatgctcat cgagctggac gccatccacg acaagatcgg
     1021 gatcaccttc atctacgtga cccacgacca gcaggaagcc ctgtccgtct cggaccggat
     1081 cgcggtcatg aacgccgggc aggtcctcca gatcggcacc ccccacgaga tctacgaaag
     1141 cccggccacc ggcttcgtgg ccgccttcat cggggagacg aacatgttcg aggggaccgt
     1201 ggagtccgtg gagggcacca tggtggccgt ggacgtgacg ggcctgggcc gcatcctggt
     1261 gaccgaggac gaacccccgg cgcccgggtc caaggcctcc gtggtcgtgc ggcccgagaa
     1321 gatccgcatc gacaccgagc gtccggcgcc cgccaagcgc acggacatca atttcctgga
     1381 aggctccgtg gacgaaccca tctactcggg atcgagcacc aagtactatg tccggcacca
     1441 gtccgggacc atgctgaagg tgatcaagca gcacgccaac tggtcggacg agggacccga
     1501 gatcgagtgg aaggacaagg tctacctatc ctggagcgcc gacgacgcct acctcctggg
     1561 agcgtcctga tggctcccat ggcggtccgc tcccgcacga gcaggggtcc cggcagggtc
     1621 cggaactacg gccccgccta cgcgggaccc gcgggcatct ggctgacggt cttcttcgtc
     1681 gcccccctcc tggtcatcgt ggcctacagc ttcatggcga aggggctgta cggaggagtg
     1741 gagccgaggt tctccctgga ggcctaccgg tccctggcca actcggtctt cctccgggta
     1801 tccatgaata ccctggggat ctccctggtc accacggtcc tggccatcct gatcgccctg
     1861 ccctgcggct actacatggc ccggtccaac cggaagaccc tcctgctcat gctggtgatc
     1921 atccccttct ggacgaattt cctgatccgc atctacgcgt ggatcgccat cctggggaac
     1981 aacggcttct tgaacgccct gctcctacgg gcgggcatcg tcgccgcccc cgtccagttc
     2041 ctgtacaacc aggtggccgt aaccatcgtc ctggtctaca cctatctgcc ctacgcgatc
     2101 ctccccctct actccacgat cgagaagttc gacttctccc tcctggaggc ggcccgggac
     2161 ctgggctgca cccggcgcgg ggcgatcttc cgggtcctcc tcccgaacat ccgggcgggg
     2221 atcatcaccg cggtactctt cgtcttcatc ccgaccttcg gggcctacgc ggtccctctg
     2281 ctcgtaggcg gcaaggaatc ctacatgctg ggcaacttcg tggccgagga gctcacccgc
     2341 acccgcaact ggcccctggc ctccgccatc tccatggtca tcaccctggt cacgaccctg
     2401 ggcctctggc tcatgttccg gctccagggc aaggcccccc aagccaagga tcccgcccgg
     2461 tccggcgggg aacggagggc tccgtgaggg tcgtcaaagg cagggggttc tcccggaccg
     2521 tattcttcct gaccatgggc ttcctcttcc tgcccctggt ggtgctgatc gtctactcct
     2581 tcaattccgc caagagcatg agctggggcg ggttctccct ggtgtggtac gagcggctct
     2641 tcctcaagga gcggaacgtc tggaaggcct tcgagaactc cctcatcatc gcggtgtcca
     2701 gcgcggcggt cgccacctgc ctgggcaccc tgggagccat cggggtgaac tggtacaagt
     2761 tccggttcaa gaagtacgtg caggccgtcg ccttccttcc cctcatcatg ccggagatca
     2821 tcatcggcgt gtccctgttg atcttcttcg cgggcatcca gatgaagctg gggctcctga
     2881 cgatcttcat agcccacacc tccttcaacc tgcccttcgt cttcctgatg gtcctggccc
     2941 ggctggacga attcgactac tccatcatcg aggcgtcccg ggacctgggg gccacggagc
     3001 gccagaccct ct
//
LOCUS       DAOTEI010000267         2991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_25786_length_2991_cov_3.834469, whole genome shotgun sequence.
ACCESSION   DAOTEI010000267 DAOTEI010000000
VERSION     DAOTEI010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25786_length_2991_cov_3.834469"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="P5313_16425"
     CDS             complement(<1..1578)
                     /locus_tag="P5313_16425"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRY82002.1"
                     /translation="MNGTLQRNLLAISTTDPELAGRISKAARDPCVHRETARSGDPIP
                     LLRGPSGDLALHSRVDPIREAERLAAAYPAGGFIVFLGLGAGYGIRPFLDRPTTGGVL
                     VVEYSLGLLRAILEEFDLAPVLSDRRVRLLADPDEPTLERAIFEAYIPILTGDLAAVP
                     LRARVDSDPGNFSEAVDAVRRVISRVSDDYSVQAFFGRRWFSNTVRNLYAAETSVRPL
                     APVQEAIVTAAGPSLDGLAPELDKARKGRYLIATDTSMGALLARGIVPDAVVSIDCQH
                     ISYYHFLNPVPDGVPLVLDLASPPSVSRRARRVHFFSSGHPFCRFVSAHFRPFPSLDT
                     SGGNVTHAAVSLADTLGARRIFLYGADFSYPRGASYARGTYIHRYFRLRAGRKSPQEG
                     LFASFLYRNLQVDRESDPDGTFRYVTKPLQAYRQRLEAFAATLSGELIPVRGSGVEIR
                     VPRIAAGPVDSSRRLSLFAPGRATCTAREFLESYRDGLASLPELSAPSILSLRNLPPE
                     KQDLWTTLLPAAAALRKG"
     gene            complement(1575..>2991)
                     /locus_tag="P5313_16430"
     CDS             complement(1575..>2991)
                     /locus_tag="P5313_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRY82003.1"
                     /translation="LGGGEDWLSEAARARLPGSLVISIQLDPRFRGRERLGADLRWYP
                     DDPIALGPFLARSVPGRLDGGVAVVSWKPSERAFPDATGTVFRELRLALEDLTSDAAT
                     VRYWSRRWMANGLRNFLAADRYCRPKPGPFPIVVAAAGPSLESALDALEPHRGRLRLW
                     ALTSAAAACARRGWTPELQVSTDPGFWADRHFDSMLRTRPSRRIPLAAHLTARISPAA
                     LGLCSLALLSPADAPENDLSRAAGLLSVPGPSRGTSAADALALAVSVSRGAVIAAGLD
                     LASRDLRSHCRPYGFDNQVLRPERRLRPGLTLIWDREIPAFPERLGIWRRGRAFDLYA
                     GGLFPAPGKGILRLLPSPVDVAGTLPLAPEDLDSVLAGDTVDPDRQVEEYEAPDRPRR
                     TSIARAAMDAWEAEAEAALSGRIGPPLPARTRRLLYALGGPEAAAFLAETARGIREEA
                     LARRARTAVEGSLAGLRERTA"
BASE COUNT          499 a          980 c         1122 g          390 t
ORIGIN      
        1 ccctttgcgc agggctgcgg ccgcgggcag gagggtcgtc cacaggtcct gcttctccgg
       61 cggaagattc cgcaggctga gaatggaagg agccgacaac tccggaaggc ttgcgagccc
      121 gtcccggtag gattccagga attcccgggc cgtacaggtc gcccggcccg gggcgaacag
      181 cgacagcctg cgggacgagt cgacgggacc ggcggcgatc cggggtacgc ggatttccac
      241 cccgcttccc cggaccggga tcagctcccc ggagagggtg gccgcgaagg cctccagcct
      301 ctgccggtag gcctggaggg gcttggtcac gtaccggaat gtcccgtccg gatcggattc
      361 ccggtccacc tggagattcc ggtacaggaa ggacgcgaag agcccctcct ggggagactt
      421 cctcccggcc ctgagccgga aataccgatg gatataggtt ccccgggcgt agctggcacc
      481 ccggggatac gagaaatcgg cgccgtacag gaaaattctc cgcgctccca gggtgtcggc
      541 cagggagacc gccgcgtggg taacgttccc cccggaggtg tccagggacg ggaagggccg
      601 gaagtgggcg gacacgaagc ggcagaaagg gtggccggag gaaaagaaat gcacccgccg
      661 tgcccgccgg gacaccgagg gaggggatgc caggtccagg accaggggca ccccatcggg
      721 cacggggttt aggaagtggt agtaggaaat gtgctggcag tctatggaga ccacggcgtc
      781 cgggacgatc ccccgggcta ggagggcccc catgctggtg tccgtggcga tcaggtaccg
      841 gcctttccgg gccttatcca gttccggggc aaggccgtcc agggacggcc ccgctgcggt
      901 cacgatcgct tcctgcacgg gtgccagggg ccgtaccgag gtctccgcgg cgtacaagtt
      961 gcggacggtg ttggagaacc agcggcggcc gaagaaggcc tggacgctgt agtcgtcaga
     1021 tacccgggag atgacccgcc gcacggcgtc cacggcttcc gaaaaattcc ccggatccga
     1081 gtccacccgg gcccgaaggg ggaccgccgc caagtctccg gtcaggatgg gtatgtaggc
     1141 ctcgaagatc gcccgctcca gggtcggttc atccggatcc gccaaaaggc ggaccctccg
     1201 gtccgacagg accggggcca ggtcgaactc ctccaggatc gcccgcagga gccccaggga
     1261 gtactcgacc acgaggactc cccccgtggt gggacggtcc aggaagggtc ggatcccgta
     1321 ccccgcgccc aggcccagga acacgatgaa gccgccggcg gggtatgccg cggccagtcg
     1381 ctccgcctcc cgaatcggat ccacccggga atgcagggcc aggtccccgg agggcccccg
     1441 tagcaggggg atggggtcgc cgctgcgggc ggtttcccgg tggacgcacg ggtcccgggc
     1501 ggccttggat atgcggcccg ccagttccgg gtccgtggtg gagatggcca ggaggttgcg
     1561 ctgaagggtg ccgttcatgc cgtacgctcc cggagtcccg ccagggaacc ttcgacggcc
     1621 gtcctcgcgc ggcgggcgag ggcctcctca cggattcccc gggccgtctc cgccaggaag
     1681 gcggccgcct cgggacctcc cagggcgtag aggagccggc gggttcgggc gggcagggga
     1741 ggaccgatcc gaccggaaag ggccgcctcc gcctcggctt cccaggcgtc catagccgcc
     1801 ctcgcgatgg aggtgcggcg cggacggtcg ggggcttcgt attcctccac ctgccggtcc
     1861 ggatcgaccg tatccccggc gaggacggaa tccaggtcct ccggagccag gggaagggtc
     1921 cccgctacgt cgacggggga cggaagtagg cgcaggattc ccttgcccgg ggcggggaag
     1981 agtcctcccg cgtagaggtc gaaggcgcgc ccccgtctcc agatgcccaa ccgctccggg
     2041 aaggcgggga tttcccggtc ccagatcaag gtcaggcccg ggcggagcct ccgttccggc
     2101 ctcaggactt ggttgtcgaa gccgtagggt ctgcagtggg accggaggtc tcgggaagcc
     2161 aggtcgagcc ccgcggcgat gacggctcct cgggagactg acacggccag ggccagggcg
     2221 tcggccgcgg aggttccccg ggaaggaccc ggaacggaga gaagccccgc ggccctcgac
     2281 aggtcgtttt caggagcgtc cgccggggaa agcagggcca gggaacagag accgagggcc
     2341 gcgggcgaaa tccgggcggt gaggtgcgcg gccaggggaa tccggcggga gggtcgggtt
     2401 cggagcatcg agtcgaaatg acggtccgcc cagaatcccg ggtccgtgct cacctgaagc
     2461 tccggcgtcc agcccctccg ggcgcaggcc gccgcggccg aagtcagggc ccacaggcgg
     2521 agccgccctc gatggggttc cagggcatcc agggccgact ccaggctcgg acccgccgcg
     2581 gccaccacga tcggaaaagg tccgggcttg ggccggcaat accggtcggc ggccaggaaa
     2641 ttgcgcaggc cgttggccat ccagcgccgg gaccaatagc ggaccgtggc ggcgtccgag
     2701 gtgaggtcct ccagggcgag ccggagctcc cggaacacgg tcccggttgc gtccgggaag
     2761 gctcgttcgg agggcttcca ggaaaccacg gcgacgcctc cgtccagacg accgggtacg
     2821 gaacgggcga gaaacgggcc cagggcgatc gggtcgtccg ggtaccaccg gaggtctgcg
     2881 cccaggcgtt cgcggccgcg gaaacggggg tccagttgga tcgagatcac cagggagccg
     2941 ggcagcctgg cccgggcggc ctccgagagc cagtcctcgc cgccgcccag g
//
LOCUS       DAOTEI010000268         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_25888_length_2983_cov_5.249658, whole genome shotgun sequence.
ACCESSION   DAOTEI010000268 DAOTEI010000000
VERSION     DAOTEI010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25888_length_2983_cov_5.249658"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1407)
                     /locus_tag="P5313_16435"
     CDS             complement(127..1407)
                     /locus_tag="P5313_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY82004.1"
                     /translation="MRSGIGLRGLFPREIYALFVVRLVVSAGSFVNPFLAMMLTMKLG
                     YSQAAAGAFMSAVSVVSAAGLLAGGKLGDSLGRRPVLGGLQALTAASFAVCAVLGFVP
                     AAPFVIAFALGVLSGTWPVMNAVVADCAPPERRKEAFALLYWGNNIGFSIGPLAAGFL
                     FSKAPRLLFVGNAAALSIAAAVVFLFVRETNPLLSAPHGAPDAEKAAAEDAGRTSSGD
                     RAGKSAGGTLRILAADPVLLVFAALAALTAFVYNQHTFALPLFLKDLFGPEAGPRAFG
                     AAMAVNGLTVVVCTALVTAASHRWPALLAAAAGSVFYLLGFGAYALAAGLIPVLACTF
                     AWTIGEILGATNNNVFIAERSPASHRGRINSAISFCYILGNALAPLVSGPAMEALGSR
                     AIWGPIGFLAGVGALGLAALHVWDRSRGPAKAAL"
     gene            complement(1500..>2983)
                     /locus_tag="P5313_16440"
     CDS             complement(1500..>2983)
                     /locus_tag="P5313_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82005.1"
                     /translation="EGIVVRNGLILGTLPEEESGRWQRLKSSADGNPGNSGGPLITPD
                     GRVVGVVAALKDNILYSLPVSALLEVPPETLKFRQRLTYGHLLLANTLTRVYETEVAL
                     PAAYREVRKTVTDRYSPVYDQTMKELFKAAPAYLDGPANAYFLSAVADSSFPELAFVD
                     KDDNQWKMSDLKVERFNLPDDGAVFQAKVGSFSFLKVELPKTVVLDSVLKDPKALMDL
                     ILKGVSLERSLGGSEKYRILSFGKPVETGEYRDMVGRTWLRARWTVEFEDRALIAYAL
                     PLPDGPAVVWTYQPTGRIGVYDWDLKAVCDRVQTGYKGTFPEWERFLGLGSLLPEGFS
                     GVRFRWSEPDRTVELKVPDFSFSADMRTFEWTPASSLFLSPAYYFQGGTVRFGFRKLL
                     IQRDVRGRDFAILYKNVKPDTRLGAKAAEGWDALVKARSPFDGKPGINPKDNVGALGA
                     ILPQREPASDALYSLYLSMEEPGSEEALAARFEALKSGIGIVR"
BASE COUNT          522 a         1011 c         1034 g          416 t
ORIGIN      
        1 tacataacgt ccccgggatc gccttcccgg aacaccgcct cgccggccag gaatattttc
       61 tcgttcatgg tgccgcccat ccctccattg taggccgccc ggggccgctt cacaagcttc
      121 gggaccctag agcgccgcct ttgcgggacc tcggctccgg tcccagacgt gcagggccgc
      181 cagccccagg gccccgaccc cggccaggaa cccgatgggt ccccagatcg cccgggagcc
      241 cagggcctcc atggccggcc ccgacaccag gggcgccagg gcgttgccca ggatgtagca
      301 gaaggagatg gcgctgttga tccgtccccg atgggaggcg ggagaccgct ccgcgatgaa
      361 gacgttgttg ttcgtggccc ccaggatctc cccgatcgtc catgcgaagg tgcaggccag
      421 gacgggtatc aaacccgccg ccagggcgta ggctccgaag cccaggaggt agaagaccga
      481 acccgccgcc gcggccaaca gggcgggcca gcgatgggac gccgcggtga ccagggcggt
      541 gcagaccacc acggtgagtc cgttcaccgc catggccgcc ccgaaggccc gggggccagc
      601 ctcgggtccg aacaggtcct tcaggaagag tggcagggcg aaggtgtgct ggttgtagac
      661 gaaggccgtc agcgcggcca gggcggcgaa gaccaggagg accggatcgg cggccaggat
      721 gcgcagggtt ccccccgcgg acttcccggc ccggtccccg gaggatgtcc ggcccgcatc
      781 ctccgcggcg gctttttccg cgtccggggc gccgtgcggg gcggacagga gggggttcgt
      841 ctcccggacg aaaaggaaca cgacggccgc ggcgatggac agggccgcgg cgttccctac
      901 gaagaggagt cggggggcct tggagaagag gaaccccgcg gccaggggac cgatcgagaa
      961 gccgatgttg tttccccaat agaggagggc gaaggcctcc ttccgccgct ccgggggggc
     1021 gcagtccgcg accacggcgt tcatgaccgg ccaggtgccc gaaaggaccc ccagggcgaa
     1081 ggcgatgacg aagggagccg ccggcacgaa gcccaggacg gcgcagaccg cgaaggaagc
     1141 ggcggtcaag gcttgcaggc ctcccagtac gggtctgcgg cccagggagt ccccgagctt
     1201 gccgcccgcc agcaaccccg cggcggagac gacggagacc gcggacatga aggctcccgc
     1261 ggccgcctgg ctgtagccca gcttcatggt cagcatcatg gccaggaagg gattcacgaa
     1321 gcttccggcg gataccacca gccggacgac gaagagggcg tagatctccc gcgggaagag
     1381 gccccgcagg ccgataccgg aacgcattct actacctcga tgcgggcccg gcggcggccg
     1441 agcccgcggc atggcgcaca tggacggcgg gaggtccatc ccgccgcccc ggactcagtt
     1501 cagcgaacga tcccaatccc ggacttgagg gcctcgaagc gggcagccag ggcttcctca
     1561 ctgccgggct cctccatgga gaggtagagc gagtacaggg cgtccgatgc cggctcccgc
     1621 tggggcagaa tggcccccag ggctcctacg ttgtccttgg gattgatccc cggcttgccg
     1681 tcgaagggcg agcgcgcctt gaccagggcg tcccagccct ccgcggcctt ggcgccgagc
     1741 cgggtgtcgg gcttcacgtt cttgtacagg atggcgaagt cccggccccg gacgtcccgc
     1801 tggatcagga gcttgcggaa accgaagcgg accgttcccc cctggaagta gtaggccgga
     1861 ctcaggaaca gggaagaggc cggggtccac tcgaaagtcc gcatgtccgc ggagaacgag
     1921 aaatccggaa ccttaagctc cacggttcgg tccggttcgg accaccggaa cctcaccccg
     1981 gaaaaacctt ccggcaggag acttcccagg cccaggaacc gctcccactc ggggaaggtc
     2041 cccttgtacc ccgtctgcac ccggtcgcag accgccttga ggtcccagtc gtacaccccg
     2101 atccttcccg tgggctgata ggtccagacg accgccgggc cgtccggcag gggcagggca
     2161 taggcgatca gggcacgatc ctcgaactcc acggtccagc gggcccgaag ccaggtccgg
     2221 ccgaccatgt cccggtactc ccccgtctcc acgggcttgc cgaaggagag gatccgatac
     2281 ttctcgcttc cccccaggct ccgctccagg gagaccccct tgaggatcag gtccatcagg
     2341 gccttgggat ccttcaagac cgagtcgagg accacggtct tgggaagctc gaccttgagg
     2401 aaggaaaagg agcccacctt ggcctggaaa accgccccgt cgtccggcag gttgaaccgc
     2461 tccaccttca ggtcgctcat cttccactgg ttgtcgtcct tgtccacgaa ggccagctcg
     2521 gggaaggagg aatccgccac cgcggagagg aagtaggcgt tggcggggcc gtccaggtag
     2581 gcgggggcgg ccttgaagag ctccttcatc gtctggtcgt agaccgggga gtaccggtcc
     2641 gtgacggtct tccggacctc ccggtaggca gccggcaggg cgacctcggt ctcgtacacc
     2701 cgggtcagag tgttggccag gaggaggtgc ccgtaggtca gacgctggcg gaacttcagg
     2761 gtctccgggg gaacctcgag cagggcggag acgggcaggg agtagaggat gttgtccttg
     2821 agggccgcca cgacccccac gaccctgccg tccggggtga tcaggggccc cccggagttg
     2881 ccggggttcc cgtccgccga ggacttcagg cgctgccagc ggccggactc ctcctcgggc
     2941 agggtgccca ggatgagccc gttccgcacc acgatgccct ccc
//
LOCUS       DAOTEI010000269         2935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_26509_length_2935_cov_5.301389, whole genome shotgun sequence.
ACCESSION   DAOTEI010000269 DAOTEI010000000
VERSION     DAOTEI010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26509_length_2935_cov_5.301389"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="P5313_16445"
     CDS             complement(<1..1033)
                     /locus_tag="P5313_16445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY82006.1"
                     /translation="MPDTEPVCRKTPDPAGDGSGAILPVAVATRFLQWGSLGLILPVS
                     NLFRLSKGLSLGELGFSAAFVSAVVILLEVPTGILADRIGRRRVYLASLAFFALSCLT
                     LLASRGFLSVTVGFALYGVSRALSSGSLEALLIDRYLESEGEGRLHRLMAATNAADTA
                     GLALGSLAGGFVPMGWSILAPGAGRYDGNLIAMLVLTAILAAVVILAVPEEPPRAGER
                     EKVGGFLAGSLRAITGSRELALILATMIAWGFAFSAVETYWQPRLADILGSSESTWVF
                     GAVSTGYFLAALLGSLASPLVLERTRISPYLLLFVLRLTTAGFIVLLALQGSATGFAV
                     FYLAMFCWNG"
     gene            complement(1026..1865)
                     /locus_tag="P5313_16450"
     CDS             complement(1026..1865)
                     /locus_tag="P5313_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82007.1"
                     /translation="MDSKVVNERAADLRDSSIRVTPALLEELGIVKSDTVLRVGDFSV
                     SCVPFELSLSRMELLAVLSEREAEFFQRYLGQSHRLFMTCRPPYGSKPHAFFLTCRIS
                     GIRRPSPVSPYCFLGLELVRSPLSYKEMLVGYFLETEKSRDFFETAPEIELSPEQRSA
                     LLGGGYLQLLLEEGEERRFKVLSFTPRRVTLFGEYGDSIPADGAVLEFRPTDTDEDFE
                     LKGVCLRSRPSVEAPGFSTLELELRFTPGAFARIRRVVGWMPGIGRNGQAGARIQDRR
                     GCA"
     gene            2023..>2935
                     /locus_tag="P5313_16455"
     CDS             2023..>2935
                     /locus_tag="P5313_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRY82008.1"
                     /translation="MSRGMGCRYGSHRVLEPRGILPQPAIRLDNSTEIYDNEVLIDVG
                     TLNIDSASYTQIRAACGGDADRMKDMILGIVRERGKMQNPVTGSGGMLVGTVRDIGPA
                     FPDKKLAVGDRIATLVSLSLTPLSIEKILRLDPESDQVDVEARAILFESGIYAHMPPD
                     LPEKLALAVLDVAGAPAQVDRLVKEGDTVCIIGGGGKSGVLCCYQAMKNTGPYGKVIV
                     VEYSPENARRIRDMRLATDVIVADATNAIDVYHRVLEITGPRGCDVTINNVNVPNTEM
                     TSILITKGQGLIYFFSMATSFTKAALGA"
BASE COUNT          583 a          984 c          942 g          426 t
ORIGIN      
        1 tcccgttcca gcagaacatg gccagataga acaccgcgaa acctgttgcc gagccctgca
       61 gggccagcag gacgatgaaa cccgccgtag tcaggcggag gacgaacagc aggagatagg
      121 gcgatatccg ggtccgctcc aggaccaggg gagatgccag actgcccagg agggccgcca
      181 aaaaataccc cgtggatacc gcgccgaaca cccaggtgga ctccgaggaa cccaggatgt
      241 ccgccagccg tggctgccag taggtctcga cggcgctgaa ggcgaagccc caggcgatca
      301 tcgtggccag gatgagcgcg agctcccggc tgcccgtgat cgcccgcagg gagcccgcca
      361 ggaatccgcc taccttttcc ctctcccccg cccggggcgg ctcctcgggc acggccagga
      421 tcacgaccgc ggccaggatc gcggtcagga ccagcatggc gatcaggttg ccgtcgtacc
      481 ggcccgcgcc gggcgccagg atcgaccatc ccatagggac gaagcctccc gccagggatc
      541 ccagggccag tcccgccgta tccgcggcgt tggtcgccgc catgaggcgg tggagcctgc
      601 cttccccctc gctctccagg tatcggtcga tcagcagggc ctccagggaa cccgaggaca
      661 gggcccggga gacgccgtac agggcaaagc ctacggtcac ggacaggaag ccccgggacg
      721 ccagcagggt caggcaggac agggcgaaga aggccaggga tgccaggtag accctccttc
      781 gaccgatcct gtccgccagg atccccgtgg ggacttccaa caggattacc accgcggaaa
      841 cgaaggccgc gctgaacccc aactccccca gggagagccc cttggacagg cggaagaggt
      901 tcgacacggg caggatgagc cccagggaac cccactgcag gaagcgggta gcgacggcga
      961 cgggcaggat ggctccggac ccgtcgcccg caggatcggg ggtcttccgg cagacaggtt
     1021 cggtatcagg cacaacccct cctgtcctgg atccgggccc cggcctgacc gttccgcccg
     1081 atcccgggca tccagccgac gacccgtcgg atccgggcga acgcgccggg cgtgaatcgc
     1141 agctccagtt caagagtgga aaaaccgggc gcttccacgg acggtcggga ccgcaggcag
     1201 acacctttca actcgaagtc ctcatcggta tcggtcggac ggaattcaag caccgcaccg
     1261 tcggccggta tcgaatcgcc gtattccccg aaaagcgtga cacgccgcgg ggtgaacgac
     1321 aggaccttga atcgacgctc ctctccctcc tccaagagca gttgaagata gccgcccccg
     1381 agcagggccg accgctgttc cggggaaagt tcgatctccg gggccgtctc gaaaaagtcc
     1441 ctggatttct ccgtctccag gaagtatccg acaagcatct ccttgtagga caacggggac
     1501 cgtacgagtt cgaggccgag aaagcagtac ggcgagacgg gcgagggcct tcgaattccg
     1561 gaaatcctgc aggtcaggaa aaaggcgtgg ggtttcgatc cgtacggcgg cctgcaggtc
     1621 ataaaaagcc ggtgggactg tccgaggtat cgttggaaaa actcggcttc ccgctcgctc
     1681 aggaccgcca ggagctccat ccgcgacaag gaaagctcga agggtacgca ggacaccgaa
     1741 aaatccccca ccctcaggac ggtgtccgac ttcacgatgc cgagttcctc cagcagggcc
     1801 ggggtaaccc gtatcgagct atcccggagg tccgccgccc gttcgttgac gactttcgaa
     1861 tccatcccat cggccccttc ggcatcccgc gcaggctgtg atttcggagt cggctccagt
     1921 atgcctgacc gacggtcgtc cggcaagccc ggctggtttg cctcgattgc atcgcgattc
     1981 tcccggtggt actatcccgg ggaacccgaa aggaggccgt ccatgtccag gggaatggga
     2041 tgccgctacg gaagccaccg ggtcctcgag ccccggggga tcctccccca gccggctatc
     2101 cggctcgaca attccacgga gatctacgac aacgaggtgc tgatcgacgt aggcaccctg
     2161 aacatcgact ccgccagcta cacccagatc cgggcggcct gcgggggcga cgcggaccgg
     2221 atgaaggaca tgatcctggg catcgtccgg gagcggggca agatgcagaa tcccgtgacc
     2281 ggctccgggg gcatgctggt cggcacggtc cgggacatcg gcccggcctt cccggacaag
     2341 aagctggcgg tcggggaccg gatcgcgacc ctggtgtccc tctccctcac ccccctttcg
     2401 atcgaaaaga tcctccggct ggaccccgag agcgaccagg tggacgtgga ggcccgggcg
     2461 atcctcttcg agagcgggat ctatgcccac atgcctccgg acctgccgga aaagctggcc
     2521 cttgcggtcc tggacgtggc gggagccccc gcccaggtgg accggctggt caaggagggc
     2581 gacacggtct gcatcatcgg cgggggcggc aagtccgggg tcctctgctg ctaccaggcc
     2641 atgaagaaca cggggcccta cggcaaggtc atcgtcgtgg agtactcccc ggagaacgcc
     2701 cgcaggatcc gggacatgcg cctggccacg gacgtgatcg tggcggacgc aacgaacgcc
     2761 atcgacgtgt accacagggt cctcgaaatc acgggtcccc ggggttgcga cgtgaccatc
     2821 aacaacgtga acgtcccgaa caccgagatg acctccatcc tgatcaccaa gggccagggg
     2881 ctcatctact tcttctccat ggccacgtcc ttcaccaagg cggccctggg cgccg
//
LOCUS       DAOTEI010000270         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_26736_length_2918_cov_5.212714, whole genome shotgun sequence.
ACCESSION   DAOTEI010000270 DAOTEI010000000
VERSION     DAOTEI010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26736_length_2918_cov_5.212714"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="P5313_16460"
     CDS             <1..421
                     /locus_tag="P5313_16460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY82009.1"
                     /translation="EVPLLIRPDLLAPERGARPDGGKVYSLWDAYVACDFVTYPSTYE
                     GFGNAFLEAVYYRKPILVNQYAIYAQDIDPIGFKVVSIDGFITRDTVRQTIELLADPS
                     MREDWAEHNFRLGRRFFSYDILRKRLPYLFMNFGVVS"
     gene            complement(447..1559)
                     /locus_tag="P5313_16465"
     CDS             complement(447..1559)
                     /locus_tag="P5313_16465"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRY82010.1"
                     /translation="MTPVSLVACASYDPAALRAAAARAFDLADGPDPSGKTVLLKPNL
                     LSASHPDKAVTTHPEVLRAAIRLFRERGAARILVGESPGWQSASLVAGRTGVRAVVEE
                     EGVEWADFSESAVMEVPEGRVVKRFDAARAALDADILVSLPKLKTHKLLYFTGAAKNL
                     FGIVPGLGKSRYHLRFPERREFGAMITDLVLAAKPAFSLMDAVVGMEGPGPGNGTPRP
                     LGWLLASRDCLALDWTASGLIGYEPGDIPYLADARDRGILVPRDIEVRGLDPGRERVT
                     DYKRIHVPHDTDFFRARLPGFVHRIARNLAVERPAFSDRRCVACAGCVKICPAGALAF
                     REGKRAPVIDYAACIRCYCCHEICPADAIRLIRRPW"
     gene            complement(1576..>2918)
                     /locus_tag="P5313_16470"
     CDS             complement(1576..>2918)
                     /locus_tag="P5313_16470"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRY82011.1"
                     /translation="GVVPGLHVLRFELAGHRSRELVLDLAEKRTYTIQVILVPRTGRL
                     SVSVTPTDARVSVDGSELSGSLAEVPAGIRTVRASRFGYEEAVQNVFVPEDGIASAAF
                     VLEPAPFTLSELRLKRPSFNPANTGPLGLAEIRFTVTSFGRAELEIRDSGGERVARIP
                     VPPFDRNVQILVWDGRGPDGRPLPDGEYSLELRAFPEDPGTDPSEFVRRGILRVDSTF
                     LVAPRGLSGGLSGLALFPDPFTDPGRLLRFHGAVRPAFTPAGVLDGSFISLGAQTAFG
                     PAGEAALQGSFDPGDADSGSLGAGLKLTLVRADRFQAAAFAAAALGSPGGDPVAVPPA
                     RLGFSAALGSLRAYGGLALELEVPSWADPAPVLAARAGAALAGGRFGAGLSAAIRTEA
                     LAGGFALDGTIRTALEARAFLAPLPLALGLCLGVDFDVGGLSALRTGLELSLTF"
BASE COUNT          464 a         1025 c         1063 g          366 t
ORIGIN      
        1 ggaggtgcct ctcctgatcc ggcccgatct cctggccccg gagcggggcg caaggcccga
       61 cgggggcaag gtctacagcc tctgggacgc ctacgtggcc tgcgacttcg tcacctatcc
      121 ctcgacctac gagggcttcg gcaacgcctt cctggaggcc gtctattacc ggaaacccat
      181 cctggtgaac cagtacgcca tctacgccca ggacatcgac cccatcggct tcaaggtggt
      241 gtccatcgac gggttcataa cccgggacac cgtccggcag acgatcgagc tcctggcgga
      301 cccctcgatg cgggaggatt gggcggagca caacttccga ctgggccggc gcttcttctc
      361 ctacgacatc ctgcgcaagc ggcttcccta cctgttcatg aacttcggag tcgtctccta
      421 gcgggcgctc cgggatcgcc gccgcgtcac cagggccgcc ggatcagccg gatcgcgtcg
      481 gcgggacaga tctcgtggca gcagtagcat cggatgcagg cggcatagtc gatcaccggg
      541 gcccgcttgc cctcccggaa ggcgagggcc ccggcggggc agatcttcac gcagcccgcg
      601 caggcgacgc acctccggtc cgagaaggcg gggcgctcca cggccaggtt ccgggcgatc
      661 cggtgtacga agccgggcag gcgggcccgg aagaagtcgg tgtcgtgggg gacgtggatc
      721 cgcttgtaat ccgtcacccg ctcccgcccg ggatccaggc cccggacctc gatgtcccgg
      781 gggaccagga tgccccggtc ccgggcgtcc gccaggtagg ggatgtcccc gggctcgtac
      841 ccgatcaggc ccgaggccgt ccagtccagg gccaggcagt cccgggaggc caggagccag
      901 cccaggggcc ggggcgtccc gtttccggga ccgggtccct ccatcccgac cacggcgtcc
      961 atcagggaga acgcgggctt ggcggccagg accaggtccg tgatcatggc cccgaactcc
     1021 cggcgctcgg ggaaccgcag gtggtaccgg ctcttgccca gccccggcac gatgccgaag
     1081 aggttcttgg ccgccccggt gaagtagagg agcttgtggg tcttgagctt gggaaggctc
     1141 accaggatgt ccgcgtccag ggcggcccgg gccgcgtcga agcgcttgac cacccggcct
     1201 tccggaacct ccatgaccgc ggactcggag aagtccgccc actcgacgcc ctcctcctcg
     1261 accacggccc ggacgcccgt cctgccggcc accagggagg cgctctgcca gcccggggac
     1321 tcgcccacca ggatccgggc cgcccctcgc tcccggaaca gccggatcgc cgcccgcagg
     1381 acctccgggt gggtggtaac ggccttgtcc gggtggctcg cggaaaggag gttgggcttc
     1441 aggagcacgg tcttgccgga cggatccgga ccgtccgcca ggtcgaaggc ccgggccgcg
     1501 gcggcccgga gggcggccgg atcgtaggat gcgcaggcga caagggaaac gggggtcatg
     1561 ggggctcctc ggacgtcaga aggtcaggga aagttccagg ccggtgcgga gggcggaaag
     1621 gccgcctacg tcgaagtcga cgcccaggca gaggcccagg gccaggggca ggggggccag
     1681 gaaggcccgg gcctccaggg ccgtccggat cgtgccgtcc agggcgaatc cgcccgccag
     1741 ggcttccgtc cggatcgccg ccgagagccc cgcgccgaag cggcctccgg ccagggccgc
     1801 cccggcccgg gccgccagca cgggagcggg gtccgcccag gaggggacct cgagctccag
     1861 ggccagcccg ccgtaggcgc ggaggctgcc cagggccgcg gagaagccca ggcgggccgg
     1921 gggcacggcg acgggatccc ccccggggga gcctagggcc gccgccgcga aggccgcggc
     1981 ctggaaccgg tccgcccgga ccagggtcag cttcaggccc gccccgaggg aaccggaatc
     2041 ggcgtctccg ggatcgaagg agccctgcag ggccgcttcc cctgcggggc cgaaggcggt
     2101 ctgggccccc agggagatga aggaaccgtc caagactccc gccggggtga aggcgggacg
     2161 gacggccccg tggaagcgca gaaggcgtcc cggatccgta aaggggtccg ggaagagggc
     2221 cagcccggac aatccgccgg acagcccccg gggtgcgacc aggaaggtgg aatcgacccg
     2281 gaggatcccc cgacggacga attcggaggg atccgtgccc gggtcctccg gaaaggcccg
     2341 cagttccagg gagtactctc cgtccggaag gggacgtccg tccgggcccc ggccgtccca
     2401 gactaggatc tggacgttcc ggtcgaaggg cggcacgggg atccgcgcta cccgttcgcc
     2461 tccggaatcc cggatctcca gctccgcccg gccgaaactc gtgacggtga accgtatctc
     2521 cgcgaggccc agggggcccg tgttcgcggg attgaaggac ggccgtttca ggcggagctc
     2581 cgagagggtg aagggggcgg gttccaggac gaaggccgcg gaggctatcc cgtcctcggg
     2641 gacgaagacg ttctggacgg cctcctcgta cccgaagcgg gaggcccgca cggtgcggat
     2701 ccccgcgggc acctcggcca gggacccgga cagctccgag ccgtccacgg atacccgggc
     2761 atccgtgggg gtgaccgaga cggaaagccg gcccgtccgg ggcaccagga tcacctggat
     2821 cgtatacgtc cgcttctccg ccaggtccag gaccagttcc cgggacctgt ggcccgccag
     2881 ctcgaatcgc aggacatgga ggcccggaac cacacccc
//
LOCUS       DAOTEI010000271         2871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_27421_length_2871_cov_4.225142, whole genome shotgun sequence.
ACCESSION   DAOTEI010000271 DAOTEI010000000
VERSION     DAOTEI010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27421_length_2871_cov_4.225142"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="P5313_16475"
     CDS             <1..662
                     /locus_tag="P5313_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRY82012.1"
                     /translation="AKSKTILVFINQIRMKIGVMFGNPETTTGGNALKFYASVRLEVR
                     KIETIDKGADEDAIGNKVRVKVVKNKVAPPFRKVEMEIMFGKGVSRLGSLIDAAVKYN
                     VVDKKGAWYTWGEEKIGQGRDNVKIYLEQNPDLADELERKLRQLIFGKKDEAAGDAKP
                     GAGKAAPARPEAGARPRGETAAETPPARPVGRPPAAKTAAAKPSAGPSAGPSADDEGL
                     F"
     gene            683..1174
                     /gene="folK"
                     /locus_tag="P5313_16480"
     CDS             683..1174
                     /gene="folK"
                     /locus_tag="P5313_16480"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRY82013.1"
                     /translation="MEVFLGLGGNVGDTAAAFRSAFRSLAAFLRDARISSFYRTRPRY
                     LEDQPPFLNAVVIGRTFLGPRELLAAVQGVEAGHGRDRSREIRKGPRTLDIDILLYGD
                     AVLDLPDLVVPHPGLPERLFALVPLLELAPGLVDPRTDRPFAELAAALPDQGIYLEGE
                     AGL"
     gene            1295..2098
                     /locus_tag="P5313_16485"
     CDS             1295..2098
                     /locus_tag="P5313_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRY82014.1"
                     /translation="MSDLSEYSPEPSGTGQRFRLKDFEGPLDLLLFLIKKNEVSIYDI
                     PIASITEQYLEYLDMAAETSLEELTEFYSLASTLLYIKSRMLLPVEVDLGDEIEDPRR
                     DLIEKLIEYQKFRKLSELMEEKELEVEWTIERKKIQRALPFAEEEELWTKIDVWDLLI
                     SFSGLISSVSSERIIDLYEEVSINEKLALIQEILDGHESFDFTDLLTRRKSTLDVICA
                     FLAVLEAVKLRWIAIFQHKLFGDIQIRRHDKRNERSEDTGALDGSAPGA"
     gene            2076..2624
                     /gene="scpB"
                     /locus_tag="P5313_16490"
     CDS             2076..2624
                     /gene="scpB"
                     /locus_tag="P5313_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRY82015.1"
                     /translation="MEVRLERETALLETILFLEPEPQDLTSLVRISGLGRDVVESALK
                     SLEEALAAPDHGLELVRIGGGWTLAPKRELWEGLREHYGRKKESKLSRAALETLSIIA
                     YSQPVTRAEIEALRGVGADTMIHFLLEKELIKEVGKKDAPGRPIQYGTTKDFLKYFRL
                     DSIADLPRLDELDRERFEKEEP"
     gene            2621..>2871
                     /locus_tag="P5313_16495"
     CDS             2621..>2871
                     /locus_tag="P5313_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRY82016.1"
                     /translation="MTEPGEGDAGPGSADPGDRGPRLQAYLAACGLASRRACEDLIRA
                     GRVLLDGRVVTELGTRVPPGARVEVDGKPANTETRKRYLA"
BASE COUNT          536 a          953 c          935 g          447 t
ORIGIN      
        1 tggccaagtc caagaccatc ctcgtcttca tcaaccagat ccgcatgaag atcggggtca
       61 tgttcggcaa tcccgagacc accacggggg ggaacgccct aaagttctac gcctccgtgc
      121 gcctggaggt ccgcaagatc gagaccatcg acaagggcgc cgacgaggac gctatcggaa
      181 acaaggtccg ggtcaaggtc gtgaagaaca aggttgcccc tcccttccgg aaggtggaga
      241 tggaaatcat gttcggcaag ggcgtctccc ggctgggaag tctcatcgac gcggcggtca
      301 agtacaacgt ggtggacaag aagggcgcct ggtacacctg gggcgaggag aagatcggcc
      361 agggccggga caacgtgaag atctacctgg aacagaaccc ggacctggcg gacgaactcg
      421 agaggaagct ccgccagctc atcttcggca agaaggacga ggccgccggg gacgccaagc
      481 cgggcgccgg gaaagccgcc cccgctcgcc cggaagcggg ggcacgtccc cggggggaga
      541 ccgcggcgga gaccccgccc gcacggcccg tgggccgccc gcccgctgcc aagaccgcgg
      601 ccgcgaaacc ttcggccgga ccttcggccg gaccttcggc ggacgacgag ggtctcttct
      661 gagcggggcc gcttcgtccg gggtggaggt tttcctgggg ctcggcggga acgtcgggga
      721 tacggctgcg gccttccggt ccgccttccg ctccttggca gccttccttc gggacgcccg
      781 gatctcctcc ttctaccgga cgcgcccccg gtacctggag gaccagccgc ccttcctgaa
      841 cgcggtcgtc atcggccgca cctttctggg gccccgggag ctcctggccg ccgtccaggg
      901 ggtggaggcg ggacacggcc gggaccgatc ccgggagatc cggaagggcc cccggaccct
      961 ggacatcgac atcctcctgt acggggacgc cgtcctggac ctcccggacc tcgtcgtgcc
     1021 gcatccgggg cttccggaac ggctcttcgc cctggtgccc ctcctggaac tggcccccgg
     1081 tctggtcgat ccccgcacgg acaggccttt cgcggagctg gccgccgcgc tccccgacca
     1141 aggtatttac ctggaagggg aggccggact ataatccctt cgcctcccgg aatcggccga
     1201 tcccgggagc cgcgcacggc cgccgccctg cggctccgcg tacggccgcc gcagggcggc
     1261 gaaatcccat taaaggggtt cggtacccta caccatgtcg gacttatcgg agtacagccc
     1321 cgagccctcg ggcacgggcc agcgcttccg cctcaaggac ttcgaaggtc ccctggatct
     1381 tctgctcttc ctcatcaaga agaacgaggt gagtatctac gacatcccca tcgcctcgat
     1441 cacggagcag tacctcgaat acctggacat ggccgccgag acctccctgg aagagctcac
     1501 ggagttctac tccctggcct ccaccctcct gtacatcaag tcccgaatgc tcctacccgt
     1561 ggaggtggat ctgggcgacg agatcgaaga ccctcgccgg gacctgatcg agaagctcat
     1621 cgagtaccag aaattccgca aactctcgga gctcatggag gagaaggaac tcgaggtcga
     1681 atggaccatc gaacgcaaga agatccaacg ggcccttccc ttcgcggagg aggaggagct
     1741 gtggaccaag atcgacgtct gggacctcct catctccttt tcggggctca tctcctcggt
     1801 ttcctccgag cggatcatcg acctctacga ggaggtctcc atcaacgaga agctggccct
     1861 gatccaggag atcctggacg gacacgagag cttcgacttc acggatctcc tgacccggcg
     1921 gaaatccacc ctggacgtga tctgcgcctt cctggccgtc ctggaagccg tgaagctccg
     1981 ctggatcgcg atctttcagc acaagctctt cggagacata cagatacgac gccatgacaa
     2041 gcgaaacgaa cgaagcgaag acacgggagc cctcgatgga agtgcgcctg gagcgtgaga
     2101 ccgctctctt ggagaccatt ctcttcctgg aaccggaacc ccaggacctg acatccctgg
     2161 tccgcatctc cgggctgggc agggacgtgg tggagtccgc cctgaagagc ttggaggagg
     2221 ccctggcggc cccggaccac ggtctggagc tggtgcggat cggggggggc tggaccctgg
     2281 ccccgaagcg ggagctctgg gagggcctcc gggaacacta cggccggaaa aaggagtcca
     2341 agctctcccg cgccgccctg gagaccctct ccatcatagc ctattcccag cccgtcacta
     2401 gggcggagat cgaggctctc cggggggtcg gcgcggatac catgatccat ttcctcctgg
     2461 aaaaggagct gatcaaggaa gtggggaaga aggacgcccc cggaaggccc atccagtacg
     2521 gcaccaccaa ggatttcctc aagtatttta ggctggactc catcgcggac ctcccgcgcc
     2581 tggacgaact ggatcgggag cgcttcgaga aggaggaacc ttgacggaac cgggggaggg
     2641 cgacgccgga ccgggatccg ccgacccggg cgatcgggga ccgcgtctgc aggcctacct
     2701 tgcggcctgc gggctcgctt cccgcagggc ctgcgaggac ctcatccgcg cgggccgggt
     2761 gcttctggac ggccgggtcg tgacggaact gggcacccgg gtgcccccgg gcgcccgggt
     2821 ggaggtggac ggcaagccgg cgaatacgga gacccggaag cgctacctgg c
//
LOCUS       DAOTEI010000272         2739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_29480_length_2739_cov_5.681073, whole genome shotgun sequence.
ACCESSION   DAOTEI010000272 DAOTEI010000000
VERSION     DAOTEI010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29480_length_2739_cov_5.681073"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="P5313_16500"
     CDS             complement(<1..385)
                     /locus_tag="P5313_16500"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HRY82017.1"
                     /translation="MNPERPGKETLGETILRIEDLARRDPGGRGLSGRVPAGALGPAA
                     FELLASRRVAIVTGFCVRSAGIGETDGPPGALALAGALRILGKKTALITDRYSLPLIR
                     VGAKHLGTPFRALELPEFQPEADEAL"
     gene            complement(382..1164)
                     /locus_tag="P5313_16505"
     CDS             complement(382..1164)
                     /locus_tag="P5313_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HRY82018.1"
                     /translation="MEGKTVAQIREAIARGEWRKPTSGLAPGYTQANVVILPKEYAFD
                     FLLFCARNEKPCPVIDVSEPGVVSPPVAGPGADIRIHVPKYRVYRKGEPAGEADDIRG
                     LYTPDMVTFLLGCSFTFEQDLLDNGIPVRHIELDRNVPMYVTNRECRPAGAFAGPMVV
                     SMRPIPADLVEKAAAVTKPYRKAHGAPIHAGDPAVLGIRDVMKVDFGDPPEIRPGEVP
                     VFWACGVTPQMALRRAKPEIVITHAPGHMFITDIRDKDLAES"
     gene            complement(1182..2408)
                     /gene="tyrS"
                     /locus_tag="P5313_16510"
     CDS             complement(1182..2408)
                     /gene="tyrS"
                     /locus_tag="P5313_16510"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRY82019.1"
                     /translation="MNPALETIKERGFLQQCTDLEGLSKLMDEGPVTFYIGCDPTGPS
                     LHIGHMVPFFALRHLRDAGHVGIALLGSGTARIGDPSGKTETRKMISYEAIDANAELF
                     RKQLDRFVGFDGKTSFSANNKDWLADLNYIDFLRDIGSHFSVNRMLSFEAYKARMETG
                     LSFVEFNYQLLQSYDFLELYRRHGCRLQIGGDDQWGNIVAGMELIRRIEGAEVFGLTF
                     PLVMTHDGKKMGKTEKGALFLDPAMTTPYDFFQYWRNVSDADVLKFLLMFTFLPTAEC
                     RTLASPGADINAAKERLGWEVTALIHGKDEADRALAGAKAAFGGGGDRSSMPTAELPR
                     TRFDAGYPVLDLFVDAGLAQTKSEARRLVQQGGAFVADAAWKDEKAPVGAGLLGADGE
                     LVLRAGKKRYCRVVAK"
     gene            complement(2526..>2739)
                     /locus_tag="P5313_16515"
     CDS             complement(2526..>2739)
                     /locus_tag="P5313_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82020.1"
                     /translation="CMTWGYKHVPVSRGSRIFYLETLLAIGFGALLGERLRPAFFAGG
                     ALIAAGLAFDAWDAARRKSGKILAVQ"
BASE COUNT          413 a          915 c          952 g          459 t
ORIGIN      
        1 ccagggcctc gtccgcctcc ggctggaact ccggaagctc cagggcccgg aagggagtgc
       61 ccaggtgctt ggccccgacc cgaatcaggg gcagggaata ccggtccgtg atcagggccg
      121 tcttctttcc aaggatccgg agggcgccgg ccagggccag ggcgcccggg ggaccgtcgg
      181 tctccccgat gcccgcggag cggacgcaga atcccgttac gatcgccacc cggcggctcg
      241 ccaggagctc gaaggccgcg gggcccaggg cgcccgcggg cacccgtccg gaaagtcccc
      301 ggcccccggg atcccggcgc gccaggtcct cgatccgcag gatcgtctct ccgagggttt
      361 cctttccggg ccgttcggga ttcacgattc cgccaggtcc ttgtcccgga tgtccgtgat
      421 gaacatatgg cccggggcgt gggtgatgac gatctccggc ttggcccggc gcagggccat
      481 ctggggggtg accccgcagg cccagaatac ggggacctcc ccgggccgga tctccggggg
      541 atccccgaag tccaccttca tgacatcccg gatgcccagg accgcgggat ccccggcatg
      601 gatcggggcg ccgtgggcct tgcgataggg cttggtcacg gcggccgcct tttccaccag
      661 gtccgcgggg atgggccgca tgctgaccac catggggccg gcgaaggcgc ccgcggggcg
      721 gcattcccgg ttcgtgacgt acatgggtac gttccggtcc agctcgatgt gccggaccgg
      781 gatgccgttg tccaagagat cctgctcgaa ggtgaagctg cagcccagga ggaaggtaac
      841 catgtccggg gtgtagaggc cccggatgtc gtccgcctcc ccggcgggct cgcccttccg
      901 gtagacccgg tacttcggca cgtggatccg tatgtccgcg ccgggaccgg cgacgggcgg
      961 ggagaccacc cccggctcgc tcacgtcgat gaccgggcag ggcttctcgt tccgcgcaca
     1021 gaacaggagg aagtcgaagg cgtattcctt cggtaggatg accacgttgg cctgggtgta
     1081 ccccggggcg agccccgagg tgggttttcg ccactcgccc cgggcgatgg cttcccggat
     1141 ctgggcgacc gtttttcctt ccatggcctt ccgatcctcc cctatttcgc gacgacgcgg
     1201 cagtaccgct tcttgcccgc ccggagcacg agctccccgt ccgcgccgag gagccccgcg
     1261 ccgacggggg ccttctcgtc cttccaggcc gcgtcggcca cgaaggcccc gccctgctgg
     1321 accagacggc gggcctcgct cttggtctgg gcaaggcccg cgtccacgaa gaggtccagg
     1381 acgggataac ccgcgtcgaa gcgggtccgc ggaagctccg cggtgggcat ggagctccgg
     1441 tcgccgccgc ccccgaaggc cgccttggcc ccggccaggg cccggtccgc ctcgtccttg
     1501 ccgtggatga gggccgtgac ctcccatccc agtcgctcct tggcggcgtt gatgtccgcc
     1561 cccggggagg ccagggtccg gcactccgcc gtgggcagga aggtgaacat gagcaggaac
     1621 ttgaggacgt ccgcgtccga cacgttccgc cagtactgga agaagtcgta gggggtggtc
     1681 atggccggat ccaggaagag ggcccccttc tcggtcttgc ccatcttctt gccgtcgtgg
     1741 gtcatgacca gggggaaggt gagcccgaag acttcggcgc cctcgatccg gcggatcagc
     1801 tccatccccg ccacgatgtt gccccactgg tcgtccccgc cgatctggag gcggcagccg
     1861 tggcgccgat agagctccag gaagtcgtag ctctggagga gctggtagtt gaattccacg
     1921 aaggagagcc ccgtttccat ccgggccttg taggcctcga aggagagcat gcggttcacg
     1981 gagaaatggc tgccgatatc ccggaggaag tcgatgtagt tgaggtccgc cagccagtcc
     2041 ttgttgttcg cggagaagct ggtcttcccg tcgaagccga cgaagcggtc cagttgcttc
     2101 cggaagagtt cggcgttggc gtcgatggcc tcgtaggaga tcatcttgcg ggtctcggtc
     2161 ttgccggagg ggtccccgat ccgggcggtc ccgctcccca ggagggcgat gcccacgtgt
     2221 ccggcgtccc gtaggtgccg gagggcgaag aagggcacca tgtgcccgat gtgcagggac
     2281 gggcccgtgg ggtcgcagcc gatgtagaaa gtcacgggac cttcgtccat cagcttggac
     2341 aatccctcca ggtccgtgca ctgctgaagg aagcctcgtt ccttgatggt ctccagggcg
     2401 gggttcatga cgctcctccg atgcttggga gccccgggtt ggtctggacc atctccgggg
     2461 cgggtcctgg gattataccg gggccaggct cccccctcaa gagaggctgt ccaaaaagcg
     2521 accaattatt ggacagccag gatttttccg gacttcctcc gggccgcatc ccaggcgtcg
     2581 aaggccaggc ccgcggcgat cagggcgcct cccgcgaaga aggccgggcg caggcgctcc
     2641 cccaggaggg ccccgaaccc gatggccagg agggtctcca ggtagaagat ccggctcccc
     2701 cgggataccg ggacgtgctt gtacccccag gtcatgcag
//
LOCUS       DAOTEI010000273         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_29601_length_2732_cov_4.755697, whole genome shotgun sequence.
ACCESSION   DAOTEI010000273 DAOTEI010000000
VERSION     DAOTEI010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29601_length_2732_cov_4.755697"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="P5313_16520"
     CDS             complement(<1..1318)
                     /locus_tag="P5313_16520"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY82021.1"
                     /translation="MKRFQYLPAFLLAAALLGAQGSASPEKRRDVIRYGIESELLELV
                     TALQNEKDDTFNPDLLVLLGETRSPRLREALLRFFTVREWPGAVPAAVSLLDGRDQES
                     AQSVNAALGYLSAVKAREGIPEAREIVESRESKYLSPAIRLLGRAGGAPETDLLLGLY
                     ESEESSDSLRQDVILALGEIGAQAAVESLMRTLDDTAGKKGTRMFAAEALGKIKDARA
                     VPSLVRAANGEDPQVRGAAMTALGNFPGSEADAAIVQGLRDSVPAVRLAAVKASASRS
                     IREAEPFIRFRARNDPDRKIKDEAIKALAGMGGRENLEFLRELLQDKKVDVPSRAGAF
                     SALLERDADGSRADLEKALAAEAASKDPSLLKAFQRALVQGTDKAAAPLVQTYLLNSP
                     DFGTRIAGIDWVRRNKVQSLRSAIAVMAESDKVESVLNVDGALWLCA"
     gene            complement(1400..2527)
                     /gene="tgt"
                     /locus_tag="P5313_16525"
     CDS             complement(1400..2527)
                     /gene="tgt"
                     /locus_tag="P5313_16525"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRY82022.1"
                     /translation="MDGTEIFTIHARDGRTKARTGVLELPHGRVRTPAFMPVGTNATV
                     KAVPKEYLEEMGFEIILANTYHLYLRPGHGLIREAGGLHGFTGWKGNFLTDSGGFQVF
                     SLAQFRKITQEGVRFQSHIDGSRHVLRPEDAVDIQTALASDIFMQLDVCTPYGAPERE
                     ALEALKMTSDWARRSKERWLATRGDFRGSLFGIVQGNFYPALRARSAEEILALDLPGV
                     ALGGLSVGEPFEVFEETLAYSADLLPESKPRYVMGIGTPEYILAAVENGIDMFDCVLP
                     TRTARNGLLFTFSGKLVIKNAQYERDFGPPDEECGCRVCRQYSRAYLRHLYKNGEILW
                     SILASYHNLYFLRDLVSRIRYSIESGSFGEYKKAFYAKYLG"
BASE COUNT          478 a          915 c          871 g          468 t
ORIGIN      
        1 aggcgcacag ccacaaggcc ccgtccacgt tcagcacgga ctcgaccttg tccgattccg
       61 ccatcaccgc gatcgcggat ctcagggact ggaccttgtt ccggcgcacc cagtcgattc
      121 cggcgatccg ggtcccgaag tccggactgt tcagcaggta ggtctgcacc aggggagcgg
      181 cggccttgtc ggtcccctgg accagggccc gctggaaggc cttgagcagg gagggatcct
      241 tggaagcggc ctccgccgcc agggccttct ccaagtcggc ccgggatccg tccgcgtccc
      301 gttccaggag ggcggagaag gcccccgccc gggacggtac atccactttc ttgtcctgca
      361 gaagttcccg caggaactca aggttctccc gtccccccat cccggccaag gccttgatgg
      421 cttcatcctt gatcttccgg tccggatcgt tcctcgcccg gaaccggatg aagggctccg
      481 cctcccggat cgagcgcgag gcggaagcct tgaccgccgc caaccgaacc gcggggacgc
      541 tgtccctcaa tccctggacg atggcggcgt ccgcctcgga accggggaaa ttccccaggg
      601 cggtcatcgc ggctccccga acctggggat cctccccgtt ggccgcccgt accagggagg
      661 ggaccgcccg ggcgtccttg atcttcccca gggcctccgc ggcgaacatc cgtgtcccct
      721 tcttgcccgc cgtgtcgtcc aaggtcctca tgaggctctc gaccgccgcc tgggccccga
      781 tctcccccaa ggccaggatg acgtcctgac gcagggaatc ggaggactcc tcggattcgt
      841 acaggccgag gagcaggtcc gtctccgggg cgcccccggc ccggccgagg aggcgtatgg
      901 ccggggaaag gtacttggat tcccgggatt ccacgatctc ccgggcctcg gggatcccct
      961 cccgggcctt gaccgcggat aggtagccca gcgccgcgtt cacggactgg gcggattcct
     1021 ggtcccgtcc gtccaaaagg gagaccgcgg cgggaaccgc cccgggccat tcccggacgg
     1081 tgaagaacct cagcaatgct tcccggaggc ggggactccg ggtttccccg aggaggacga
     1141 gaaggtcggg attgaaggtg tcgtcctttt cgttctggag ggccgtgacg agttccagga
     1201 gctcggactc gatgccgtac cggatcacgt cgcgccgctt ctccggggag gcagacccct
     1261 gggcccccag cagggccgcg gcaaggagga acgccggtag atactggaac ctcttcacgc
     1321 ccaccatcct ttctctccgg ttccttccgg tcacgccgcc gcggccggtc cggaaggccc
     1381 gtgcgcggcg ccggccggat caaccgaggt acttcgcgta gaaggccttc ttgtactccc
     1441 cgaaggatcc gctctctata ctatatcgga ttcgagagac caggtccctg aggaagtaga
     1501 gattgtgata ggacgccagt atggaccaga ggatctcgcc gttcttgtag aggtggcgga
     1561 gataggcccg ggagtattgc cggcagactc ggcatccgca ttcctcgtcg ggcgggccga
     1621 agtcgcgctc gtactgggcg ttcttgatga ccagcttccc ggaaaaggtg aacagaagcc
     1681 cgttccgggc ggtccgggtg ggcaggacgc agtcgaacat gtcgatgccg ttctccacgg
     1741 ccgccaggat gtattccggc gtccctatgc ccatgacata gcggggcttg gattccggca
     1801 ggaggtccgc ggagtacgcc agggtctcct cgaagacctc gaagggttcc cccacggaaa
     1861 gtccccccag ggccactccg ggcaggtcca gggccaggat ctcctcggcg ctccgggccc
     1921 gaagagcagg atagaaatta ccttgtacga tcccgaatag actgccgcgg aaatccccgc
     1981 gggtggccag ccaccgttcc ttggaacgcc gggcccagtc cgaggtcatc ttcagggctt
     2041 ccagggcttc acgctccggt gccccgtagg gggtacagac gtccaactgc atgaagatgt
     2101 cgctggccag ggcggtctgg atatcgaccg cgtcctccgg ccgtagtacg tgccgggaac
     2161 cgtcgatgtg ggactggaac ctaacgccct cctgggtgat cttccggaac tgtgccagag
     2221 aaaagacttg gaaacctccg gaatcggtca aaaagttacc cttccagccg gtgaacccgt
     2281 gcaggccccc ggcctcgcgg atcagaccat gcccggggcg aaggtacagg tggtaggtgt
     2341 tcgccaggat gatctcgaaa cccatctcct cgaggtactc cttgggaacc gccttgacgg
     2401 tagcgttggt tcccacgggc atgaaggccg gggtgcgcac cctcccgtgg ggaagttcca
     2461 ggactccggt cctggccttg gtccgcccgt cccgggcatg gatcgtgaag atttccgttc
     2521 cgtccatcgc gactcctcga ctcaggtatg ggccgtccta aacaggagaa tccccagggc
     2581 caggaagaag accaccggcg cggcggctcc ggcggccggg gtgttccgcc aatctacccg
     2641 acgaaccggc ccgacgacaa caattgtcaa aaaataaaca agatgatagc ctcgccaggt
     2701 cacggcgcag cgcccaaggc gacaaccgct tc
//
LOCUS       DAOTEI010000274         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_30300_length_2693_cov_4.642532, whole genome shotgun sequence.
ACCESSION   DAOTEI010000274 DAOTEI010000000
VERSION     DAOTEI010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30300_length_2693_cov_4.642532"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="P5313_16530"
     CDS             complement(<1..668)
                     /locus_tag="P5313_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRY82023.1"
                     /translation="MIINHNLSAMFADRNLRVNGTYIDKNMEKLSSGLRINRAGDDAS
                     GLAVSEKMRSQIRGLNQASKNAANGISFIQTTEGYLQESQDIIQRLRELAVQASNGIY
                     SSEDRMQIQVEVSQLVDEIDRIASHAQFNGMNLLTGRFARETGENIPTGSMWFHIGAN
                     MDQRERVYIGTMTAKALGVKDPGSEAIITFSGPDSANRSIGILDAALKVVNKQRADLG
                     AYQNR"
     gene            1015..1962
                     /locus_tag="P5313_16535"
     CDS             1015..1962
                     /locus_tag="P5313_16535"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRY82024.1"
                     /translation="MNSSTFTHTSSDGVGIFVRRWAGDSPPKALLLVVHGVAEHSGRY
                     ARLASALTVRGYEVWAPDHRGHGGTARASGGRGWFAARDGFRRVVDDLEEVRGRMAAE
                     RPGLPVFLLGHSMGSSLARCYVSLYGKSLAGCALSGPLALDPFLAAAGDLIVAAGRAL
                     QGGRGIANFAHGLTLGSYAKPFSPVRTPQDWLSRDPAEVDAYIADPDCGFVCTWDWYR
                     DMNEGTKFVEDPRNVARIPQALPIYIFAGAADPVGAASGGAERLAASYRAAGIRDVSL
                     RLYPGGRHESLNEVNRDEVTRDLADWLDGRAGAIRNPGS"
     gene            2036..>2693
                     /locus_tag="P5313_16540"
     CDS             2036..>2693
                     /locus_tag="P5313_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRY82025.1"
                     /translation="MLVRYSTGSDGRPVKRALVYTAAEHRIERRSIDPDALRIVEHLR
                     ANGHSAYIVGGAVRDMLQGRRPKDFDIVTDAQPPRIRKIFRNSRVIGRRFRLVHVYAG
                     RKIFEVSTYRSIADGTIGNTYGTIEEDALRRDFTMNALYFDPVDNLLVDYVGGYKDME
                     ARVIKPVIPLKTIFKEDPVRMIRAAKYAAITGFRIPFRTGWAIRSHADLLATASPSRL
                     T"
BASE COUNT          408 a          895 c          904 g          486 t
ORIGIN      
        1 cggttctggt aggcgccgag gtcggcgcgc tgcttgttga ccaccttgag ggcggcgtcc
       61 aggatcccga tggagcggtt ggcgctgtcg gggccggaga aggtgatgat cgcctcgctg
      121 cccgggtcct tgacgcccag ggccttggcg gtcatggtgc cgatgtagac ccgctcgcgc
      181 tggtccatgt tggcgccgat gtggaaccac atggagccgg tggggatgtt ctccccggtt
      241 tcccgggcga agcggccggt cagcaggttc atgccgttga actgggcgtg gctggcgatg
      301 cggtcgatct cgtccaccag ctgggagact tccacctgga tctgcatccg gtcctcggag
      361 ctgtagatcc cgttcgaagc ctgtacggcg agctcccgga gcctctggat gatgtcctgg
      421 gattcctgga ggtagccttc cgtggtctgg atgaaggaga ttccgttggc ggcgttcttg
      481 ctggcctggt tgaggccgcg gatctgggag cgcatcttct cggaaacggc gagcccggag
      541 gcgtcgtccc cggcgcggtt gatccgcagt ccggacgaga gcttttccat gttcttgtcg
      601 atgtaggtac cgttgacccg caggttgcgg tccgcgaaca tggcgctcag gttgtggttg
      661 atgatcatct agttcctcct tgaatgtatc gcgcgagaca tccttgcctc gcggttcgat
      721 cgcgcgacgc ccattcagcc ttccggcccg ggcgccgttc catccccctt tcccggcacg
      781 cccgatccgc cctcccgggc ctcgggccgt atcgcgccgg atgcgatata gtgaaggggc
      841 gcccggcagc cgcaggtgac gtccgggcgg accttctccg ctcagaatat cggcaggaag
      901 ggtgaaaaag attagaggaa agtgaatggg tgaataggtg aatgtgtgaa tgggtactgc
      961 cgtttcacct attaactatt cactattcac ctatttacca actatacttt tcccatgaac
     1021 tcatccacct ttacccacac ctcctccgac ggcgtaggca tcttcgtgcg gcgctgggcc
     1081 ggggactccc cgcccaaggc cctgctcctg gtcgtccacg gggtcgcgga gcattcgggg
     1141 cggtacgccc gcctggcttc cgccctgacc gtccggggct acgaggtctg ggccccggac
     1201 caccgggggc acggcgggac cgcccgggct tccggcggcc gcggctggtt cgcggcccgg
     1261 gacggcttcc ggcgggtggt ggacgacctg gaggaagtcc gaggtcgaat ggccgcggag
     1321 cgtccggggc tccccgtctt cctgctgggg cactccatgg ggagctccct ggcacggtgc
     1381 tacgtctccc tgtacgggaa gagcctggcg ggctgcgctc tctccgggcc cctggccctc
     1441 gatcccttcc tggccgccgc cggcgacctc atcgtagccg cggggcgggc tctccagggg
     1501 gggcggggga tcgcgaattt cgcccacggg ttgaccctgg gctcctacgc caagcccttc
     1561 agcccggtcc gcacccccca ggactggctg tcccgggatc ctgcggaggt ggacgcctac
     1621 atcgcggacc cggactgcgg cttcgtgtgc acctgggact ggtaccggga catgaacgag
     1681 gggacgaaat tcgtggagga cccccggaac gtcgcccgca tcccgcaggc cctgccgatc
     1741 tacatcttcg cgggggccgc ggaccccgtc ggggcggcct ccgggggagc ggagcgcctg
     1801 gcggcctcgt accgggccgc ggggatccgg gacgtctcgc tcaggctgta tcccgggggc
     1861 cggcacgaga gcctgaacga ggtcaaccgg gacgaagtga cccgggacct ggcggattgg
     1921 ctggacgggc gcgccggcgc gatccggaat cccggttctt gacgcggccc gatccccgcg
     1981 gagtataatc cccgcaagaa ttaccgcacc ccagcgtgca gaatcagagg tccgtttgct
     2041 cgttcgatac agtacgggaa gcgacggccg cccggtcaag cgggcgctcg tctacaccgc
     2101 ggcggaacac cgcattgaaa gaagatccat cgatcccgac gccctgcgca tcgtggagca
     2161 cctccgggcc aacgggcact cggcctacat cgtgggaggc gcggttcggg acatgctcca
     2221 gggccggcgt cccaaggact tcgacatcgt cacggacgcc caaccgcccc ggatccggaa
     2281 gatcttccgg aacagccggg tgatcggccg gcgtttccgc ctggtccacg tctacgcggg
     2341 ccggaagatc ttcgaggtct ccacctaccg ctccatcgcc gacggcacca tcgggaacac
     2401 ctacgggacc atcgaggagg acgccctccg acgggacttc accatgaacg ccctgtactt
     2461 cgatcccgtg gacaacctgc tggtggacta cgtgggcggg tacaaggaca tggaggcccg
     2521 ggtcatcaag ccggtcattc ccctgaagac gatcttcaag gaggatccgg tccgcatgat
     2581 ccgggccgcc aagtacgccg cgatcacggg gttccggatt cccttccgca ccggctgggc
     2641 catccgctcc cacgcggacc tcctggccac ggcgtctccc tcccggctga ccg
//
LOCUS       DAOTEI010000275         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_33642_length_2526_cov_3.449211, whole genome shotgun sequence.
ACCESSION   DAOTEI010000275 DAOTEI010000000
VERSION     DAOTEI010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33642_length_2526_cov_3.449211"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="P5313_16545"
     CDS             complement(<1..460)
                     /locus_tag="P5313_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82026.1"
                     /translation="MAYPSDFKSAVENAAVLNLDLIRRYRESAHGVGNPAVGRLLQSV
                     LDQKSSQADLLRTVVGSGDAGRDTPLPRETERNDPPPSPAPAAPAPGADPATDLLRMI
                     RSEEEGLGALFERLRQAAADEENRERLHSLSETSRKIGEWARDHLELLSLY"
     gene            638..1192
                     /gene="infC"
                     /locus_tag="P5313_16550"
     CDS             638..1192
                     /gene="infC"
                     /locus_tag="P5313_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRY82027.1"
                     /translation="MADNKNLRINEQIRVREVRLIDDKGEQRGIVATLEALKIAREAG
                     VDLVEVAPQSSPPVCKLLDYGKFKFEQEKKIKESRKNQKIVKIKEVRMQPKIDDHDLE
                     FKSKHVREFLEEGNKVKVTIRFRGRELAHTEIGEEVLKSVLTHIEGSYVMEKLPAMEG
                     RFMSMILTPKAVKKPVEVKPPDGE"
     gene            1227..1427
                     /gene="rpmI"
                     /locus_tag="P5313_16555"
     CDS             1227..1427
                     /gene="rpmI"
                     /locus_tag="P5313_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRY82028.1"
                     /translation="MPKMKTKKSAAKRFTLTASGKVKYKKMNLRHILTKKSAKRKRNL
                     RKAGFVDGGNVRMIKKQLLPNG"
     gene            1511..1867
                     /gene="rplT"
                     /locus_tag="P5313_16560"
     CDS             1511..1867
                     /gene="rplT"
                     /locus_tag="P5313_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRY82029.1"
                     /translation="MPRAVDGTRRKDHRKKILKLAKGFRGRRGTNHKAAKDAVAKALM
                     FAYRDRRAKKNEFRTLWIARINAACRPEGISYSRLIEGLAKAQVAVDRKVLSDMAVRD
                     APAFKAVVERAKAALA"
     gene            1869..2321
                     /locus_tag="P5313_16565"
     CDS             1869..2321
                     /locus_tag="P5313_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HRY82030.1"
                     /translation="MITLEQVRALETRVEKALSAIDRLTSENAALRDNVSREQKRAAD
                     LERTIEEYRKDQTRIEQGILHALERLNAFEDAIQDPAPSAAPPPQADKPIRSGSAAAV
                     HAAPAAPAARKPAVEPRPAEPVRPEPASSAKDGTDPAPGAEEAELEIF"
     gene            2338..>2526
                     /locus_tag="P5313_16570"
     CDS             2338..>2526
                     /locus_tag="P5313_16570"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRY82031.1"
                     /translation="MAVQTLRIELLGTSFTIQTDESKEYMDAILAHIRRRAQEVQKST
                     RVTDPLKTSILASVYIVDE"
BASE COUNT          537 a          809 c          801 g          379 t
ORIGIN      
        1 aataaaggga gaggagttcc aggtggtccc gggcccattc cccgattttc cgggatgttt
       61 cggacaagga atggagccgc tcccggtttt cctcgtctgc cgccgcctgc cgcaggcgct
      121 cgaagagggc ccccaggcct tcctcttccg accggatcat ccggagcaaa tccgtcgccg
      181 gatccgcgcc cggcgccggg gccgcgggtg ccggagaggg cgggggatcg ttccgttcgg
      241 tttcccgggg caggggtgta tccctgcccg cgtccccgga tcccacgacc gttctgagca
      301 ggtccgcctg gctcgatttc tggtccagga cggactggag gagccgtccg accgcggggt
      361 tcccgacccc gtgggcggat tcccggtacc ttcgtatcag gtccaggttg aggacggccg
      421 cgttttccac ggcggacttg aaatcggacg gataagccat atagaatcac tgtactacgc
      481 gtcatttcag ctgtcaaaca ggcccgatcg ggggtcgagg tccgaaaaat cccggtcggc
      541 agggcggaca gccgggtacg accctttgac tcgccccgtg aagtatggta aagtagccgg
      601 aaacttccta ggctaggtgt attccgaagg aggccgattg gcggacaaca aaaacttgcg
      661 gatcaacgag cagatccggg ttcgggaagt gcggcttatc gacgacaagg gcgaacagag
      721 gggcatcgtt gccaccctgg aggccctgaa gatagcccgg gaggcgggtg tggacctggt
      781 cgaggtcgcc ccccagtcga gtccgccggt ctgcaagctg ctcgattacg gcaagttcaa
      841 attcgagcag gaaaaaaaga tcaaggaaag ccgcaagaac cagaagatcg tcaagataaa
      901 ggaagtccgg atgcagccca agatcgacga ccacgatctt gagttcaagt ccaagcacgt
      961 ccgggagttc ctagaggaag gaaacaaggt caaggttacg atacggttcc ggggccgcga
     1021 actggcgcac accgagattg gcgaggaagt gctcaagagc gtcctgaccc atatcgaagg
     1081 atcctacgtc atggagaagc ttcccgccat ggaaggccgg ttcatgtcca tgatcctgac
     1141 gccgaaggcc gtcaagaaac ccgtggaagt caagccgccc gacggcgagt gattcccgta
     1201 tactacgaag ataaggatgg atcgtcatgc ccaagatgaa gacgaaaaaa tccgctgcca
     1261 agcgattcac gctcacggca tccggtaagg tgaaatacaa gaagatgaac cttcgccata
     1321 tcctgacgaa gaagagcgcc aagcggaagc ggaaccttcg caaggccggc tttgtggacg
     1381 gcggcaacgt ccgcatgatc aagaaacagc ttcttcccaa cggctagccc gcggggcgct
     1441 ccttcccccg ttcggggcgc ccggtcagga cttccgtgag aagagcgaca gatagcacag
     1501 gagcgatacg atgcctagag cagtagacgg cacccgccgc aaggaccata ggaagaaaat
     1561 cctcaagctg gccaaggggt tccgcggccg ccgcggcacc aaccacaagg cggccaagga
     1621 cgccgtggcc aaggccctga tgttcgcata ccgcgaccgc cgggccaaga agaacgagtt
     1681 ccgcaccctc tggatcgccc gcatcaacgc ggcttgccgt cccgagggca tctcctacag
     1741 ccgcctgatc gagggcctcg ccaaggctca ggtggccgtg gaccgcaagg tcctctcgga
     1801 catggccgtc cgggacgcgc cggccttcaa ggccgtcgtg gagcgggcca aggcagccct
     1861 ggcctaggat gatcaccctc gagcaggtcc gggccctgga gacgcgggtc gagaaggccc
     1921 tgtcggccat cgaccggctc acctccgaga acgccgccct gcgggacaac gtctcccgcg
     1981 agcagaagcg ggcggcggac ctggagcgga ccatcgagga ataccgcaag gaccagaccc
     2041 gcatcgagca ggggatcctg cacgccctgg agcgcctgaa cgccttcgag gacgcgatcc
     2101 aggatcccgc tccctcggcc gccccgcccc cccaggcgga caagccgatc cgttccgggt
     2161 cggcggcggc cgtgcacgcg gccccggccg ccccggcggc ccggaagccg gccgtcgagc
     2221 ctcggcccgc cgaacccgtt cgccccgaac ccgcctcctc cgccaaggac gggaccgatc
     2281 ccgcccccgg cgcggaagag gcggagctcg agatattctg atcccgggcg cgccgccgtg
     2341 gccgtccaga ccctgaggat cgaacttctg ggcacctcct tcacgataca gaccgacgaa
     2401 tcgaaggaat acatggacgc catcctggcc catatccgcc ggcgcgccca ggaggtccag
     2461 aagagcaccc gggtgacgga tcctttgaaa acctccatcc tggcgagtgt ctatatcgtg
     2521 gacgag
//
LOCUS       DAOTEI010000276         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_37407_length_2365_cov_4.500433, whole genome shotgun sequence.
ACCESSION   DAOTEI010000276 DAOTEI010000000
VERSION     DAOTEI010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37407_length_2365_cov_4.500433"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="P5313_16575"
     CDS             complement(<1..490)
                     /locus_tag="P5313_16575"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRY82032.1"
                     /translation="MSEIRTFAVVGLGSFGAAVCETLAEKGASVIALDRDPASVERIK
                     GLVSAAVLLDSTDEDALLKAPLQDVDVAIVAIGDDLEASILTTALLRQQGVPYIAARA
                     VSAIHGTVLRRVGANEVINAEVDMGTRTARRLVAPDVLDWVPLSAEVSLTEMLAPEYF
                     AGK"
     gene            complement(556..2280)
                     /locus_tag="P5313_16580"
     CDS             complement(556..2280)
                     /locus_tag="P5313_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRY82033.1"
                     /translation="MDRRGRLVLFAFTLSVAGLFLEQTEPSPSLSVALHVLDFAVLGL
                     ILWEAAAGIAGAAYRWNHVRRNAPSLLFALGFTALFAYTKYQAFSGVPEVARGLLPQL
                     LRNAFLVLKIAGRVRRLSAFAEKFSVHPAQTIVASFLLVILTGSLLLMMPPATPDRKG
                     LGFLDAVFTSTSAVCVTGLVVADTAVDLAVPGQLVVLALIQIGGLGIMAYSFFGLFAL
                     GRKVSIRDRLTVSYMLSEDDLRGLSASLRRIVLYTFAIEGLGAAAFYPRFAALGGHGW
                     ESVFRALFHAVSAFCNAGFALFSDSLESFRSDPWIVLTAAALIILGGISFGVIRDGLR
                     SAGAALGALFGRRPAALPGPSQNTRAVVTITLALLASGLGGFYLLEHEGVMASYGLGE
                     QYLAAFFQSVTLRTAGFNTVPFGALRDSTLLFMIVFMFIGGASGSTAGGIKVNSLAAI
                     GAYFASFFRRERTPRIGGWSVAPEKVGRSLLVLAFGLAAVFLGVFLLTLTEGGDFLPL
                     LFETVSAFGTVGLSTGLTSGLSSPGKIVIIALMFLGRLGPLTILSAARKPGKRTEVQY
                     PEGDLAVG"
BASE COUNT          459 a          796 c          798 g          312 t
ORIGIN      
        1 acttgcccgc gaagtattcc ggcgccagca tctccgtcag ggacacctcc gcggaaaggg
       61 gcacccagtc caggacatcc ggagccacca ggcgtcgtgc ggtgcgcgtc cccatgtcga
      121 cctcggcgtt gatcacctcg ttggccccca cccgccggag tacggtcccg tggatggcgg
      181 agaccgcccg ggccgcgatg tagggcaccc cctgctggcg gagcagggcg gttgtcagga
      241 tgctggcctc caggtcatcc ccgatggcca cgatggccac gtccacgtcc tggagggggg
      301 ccttgagcag ggcgtcctcg tcggtggaat ccaggaggac cgccgcggaa accaggcctt
      361 ttatcctctc caccgacgcg ggatcccggt ccagggcgat cacggaggcg cctttttccg
      421 ccagagtctc gcacaccgcg gccccgaagg atcctaatcc caccaccgcg aaggtcctga
      481 tctcggacat agggcagccc tcctcgcatg aagtatccgc ggcgtccttt ccggagccgc
      541 cgcgccgccg tcccgtcagc ctacggccag atccccctcg ggatactgta cctccgtccg
      601 ctttccgggc ttccgcgccg cggagagtat cgtcaggggc cccaggcgcc ccaggaacat
      661 cagggctatg atgacgatct tgcccgggga ggacaatccg gaggtcagcc ccgtcgaaag
      721 tcccacggtc ccgaaggcag agaccgtctc gaacaggagg ggtaggaagt ccccgccctc
      781 ggtgagggtc agcaggaata cgcccaggaa gaccgcggcc agtccgaagg ccaggacgag
      841 cagggaacgg cccacctttt ccggggccac cgaccagccg ccgatccggg gcgtccgctc
      901 cctccggaag aaggaggcga agtaggctcc gatggccgcc agggagttca ccttgatacc
      961 ccccgcggta ctgccggaag cccccccgat gaacatgaag acgatcatga agaggagggt
     1021 ggagtcccgg agggccccga agggcacggt gttgaagccc gcggtgcgca gggtcacgga
     1081 ctggaaaaag gccgccaggt actgctcccc gaggccgtag gacgccatga cgccctcgtg
     1141 ctccaggagg tagaagcccc ccaggcccga agccaggagg gccagggtga tcgtgaccac
     1201 cgcccgggtg ttctgggagg gtcccggcag cgcggcgggc ctcctgccga agagagcccc
     1261 cagggccgcc ccggccgatc gcaggccgtc ccggatcacc ccgaagctga tcccccccag
     1321 gatgatcagg gcagccgcgg tcaggacgat ccagggatcc gagcggaagg attccaggct
     1381 gtccgagaaa agggcgaaac ccgcattgca gaacgccgag accgcgtgga acagggcccg
     1441 gaacacggat tcccacccgt ggcctcccaa ggccgcgaac cgcggataga aggccgcggc
     1501 tcccagcccc tcgatcgcga aggtgtacag gacgatcctc cggaggcttg cggacaggcc
     1561 ccgcagatcg tcctcgctga gcatgtacga gacggtcaag cgatcccgga tggatacctt
     1621 cctccccagg gcgaaaagcc cgaagaagga ataggccatg attcccagtc cccctatctg
     1681 gatgagcgcc agcacgacga gctgtcccgg caccgccagg tccacggccg tatccgccac
     1741 gaccaggccc gtgacgcaga ccgcggaggt ggaggtgaac accgcatcca ggaagcccag
     1801 gcccttccgg tccggcgtgg ccggcggcat catcaaaagg agggatcccg taaggatgac
     1861 caggaggaag gaggcgacga tggtctgggc gggatggacg gaaaactttt cagcgaaggc
     1921 ggaaagccgg cgaacccgcc ccgcgatctt gaggactaaa aacgcgttcc gcaggagctg
     1981 gggcaggagg ccccgggcca cctccggaac tcccgagaac gcctggtatt tcgtataggc
     2041 gaagagggcc gtgaagccca gggcgaagag cagggagggg gcgttcctgc ggacgtgatt
     2101 ccacctgtac gcggccccgg cgatcccagc cgcggcctcc cataggatca gccctaggac
     2161 cgcgaagtcc aggacgtgca gggccacgga caaggaaggg gagggctccg tttgctcgag
     2221 gaagaggccc gccacgctca gggtgaaggc gaagaggacg agtcggccgc gcctgtccat
     2281 ggaggcaagc ataccggagt gtcccgaagc ttgtgaaggc tgtccgcgcc gcctgccgtc
     2341 ctccgggccg tgtggccatt ctccg
//
LOCUS       DAOTEI010000277         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_41807_length_2207_cov_3.628717, whole genome shotgun sequence.
ACCESSION   DAOTEI010000277 DAOTEI010000000
VERSION     DAOTEI010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41807_length_2207_cov_3.628717"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="P5313_16585"
     CDS             <1..318
                     /locus_tag="P5313_16585"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRY82034.1"
                     /translation="RERKARMHELSDAFAALPGGIGTLEEFFEAWTWRALGYHAKPVG
                     ILEVDGFWEPLRIFLGGVVDGGFLGREHLADLVIRTDPGELLSALESLPPLAVHKKPE
                     RGK"
     gene            468..>2207
                     /locus_tag="P5313_16590"
     CDS             468..>2207
                     /locus_tag="P5313_16590"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRY82035.1"
                     /translation="MRRSRILASLIALIAVLPAVSQTGDFNLSVAPTFAVPIGPVIDG
                     DLQMYGIGGGATLRGDFVPGFARYLYGRASLDFDLLPLNGADDAVTLLAGGGSLGLTF
                     SPSPRLALKVGGGGGIYMAMSGLGTVRNPYVEGGAELSVRMSPSMAISLGGRYMHLFT
                     PTGPLYQGIAAQIGISYDLAGARKGTEIRLEQKINPIFPLFYSYYDKNPAGELSISNG
                     ESTPYEKVKVSFYAKQYMDGPRLCADLGTLKGGETKTAPVFALFNDSIFRVTEGTKAA
                     GEVIVEYFYLGKEKKVSFPVTVQVQNRNAMTWDDDRKVAAFVTAKDPLILGFSKNVAS
                     TVRYEGAPPVSEDFRIAFALFQALTTYNLGYAVDPKTPYTALSEKQDTVDFLQFPNQT
                     LAYRAGDCDDISVLYAALLESVGVPAALVTTPGHIFVAFDPGLPPDSAGRLFAAKGEL
                     IVRDGRAWVPVEVTLVKDGFVRAWRTGAQEWREAEAVGKAAFYPIREAWGTYEPVGFV
                     EGGIAVSLPSPERIVEGYRKEMAKFLEAQVGPRIAAIQAEDRAAKKTADQTANRIGVV
                     YAQFGLLDKAAEQF"
BASE COUNT          364 a          742 c          708 g          393 t
ORIGIN      
        1 cgggagcgga aggcccggat gcacgagctg tccgacgcct ttgcggccct gcccgggggc
       61 atcggcaccc tggaggagtt cttcgaggcc tggacctggc gggccctggg ttaccatgcc
      121 aagcccgtgg ggatcctgga ggtggacggc ttctgggagc ccctgcgcat cttcttaggc
      181 ggggtcgtgg acgggggatt ccttggccgg gagcacctgg ccgacctggt gatccgcacg
      241 gatccggggg agcttctttc cgccctggag agtctgccgc ccctggcggt acacaagaag
      301 ccggagcggg ggaagtagag gacgtcggcc attatctcca atatattaac tcctttcccg
      361 agaagagtat aattcaattc catccttgaa atacctcccg gttgtggtat tctttactat
      421 cattcacagt tccgcagcct agaatcgttt ttccatggag gccccccatg cggcggtcgc
      481 ggatcctcgc ttccctcatc gccctgatcg cggtcctccc cgcggtatcc cagaccggag
      541 acttcaacct ctccgtggcg ccgaccttcg cggtgccgat cggccccgta atcgacgggg
      601 acctccagat gtacggcatc ggcggcgggg ctacgttgag gggggacttc gtcccgggct
      661 tcgcccgcta cctctacggc cgggcttccc tggacttcga ccttctgccc ttgaacgggg
      721 ccgacgacgc ggtgaccctc ctggccggcg gcgggagcct gggactgact ttcagccctt
      781 ccccgcggct ggctttaaag gtgggcggcg gcggcgggat ctatatggcc atgtccggcc
      841 tgggcaccgt gcgcaatccc tacgttgaag gcggcgcgga gctctccgtc cgcatgagcc
      901 cctccatggc aatcagccta ggcgggcgct atatgcacct cttcactccc acgggccccc
      961 tctaccaggg catcgccgcc cagatcggca tctcctacga ccttgcgggg gcccggaagg
     1021 gcacggagat ccggctggag cagaagatca atcccatctt cccgctgttc tattcctatt
     1081 acgacaagaa tcctgccggg gaactctcga tctcgaacgg cgaatcgacc ccctacgaga
     1141 aggtgaaggt ctccttctac gccaagcagt acatggacgg accccggctc tgcgcggacc
     1201 tggggaccct gaagggcgga gagaccaaga ccgctcccgt cttcgccctg ttcaacgact
     1261 cgatcttccg ggtgaccgag ggcaccaagg ccgcagggga ggtcatcgtc gagtacttct
     1321 acctgggcaa ggagaagaag gtctccttcc cggtgaccgt ccaggtccaa aaccggaacg
     1381 ccatgacctg ggacgacgac cggaaggtcg ccgccttcgt caccgccaag gatcccctga
     1441 tcctgggttt ctccaagaac gtggcctcca cggtccggta cgagggagcc ccacccgtgt
     1501 ccgaggattt ccggatcgcc ttcgccctct tccaggccct gaccacctac aacctcggct
     1561 acgccgtgga tcccaagacc ccctacaccg ccctgtctga gaagcaggac acggtggact
     1621 tcctccagtt ccccaaccag accctggcct accgggccgg ggactgcgac gacatctccg
     1681 tgctgtacgc ggccctcctg gagtccgtgg gcgtgcccgc ggccctggtg accaccccgg
     1741 gccacatctt cgtggccttc gatcccggcc tgcccccgga cagcgcgggc cggctcttcg
     1801 cggccaaggg tgagctgatc gtccgggacg gccgggcctg ggtgcccgtg gaggtgaccc
     1861 tggtcaagga cggcttcgtc agggcctgga ggacaggggc ccaggagtgg cgggaggccg
     1921 aggctgtcgg caaggcggcg ttctatccca tccgggaggc ctggggcacc tacgagcccg
     1981 tgggtttcgt ggaggggggc atcgccgttt ccctgccgtc tccggaacgg atcgtggaag
     2041 gctaccggaa ggagatggcc aagttcttgg aggcccaggt ggggccccgg atcgcggcca
     2101 tccaggcgga ggaccgggcc gccaagaaga cggcggacca gacggccaac cggatcggcg
     2161 tggtctacgc ccaattcggc ctcctggaca aggcggccga acagttc
//
LOCUS       DAOTEI010000278         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_43090_length_2167_cov_3.794034, whole genome shotgun sequence.
ACCESSION   DAOTEI010000278 DAOTEI010000000
VERSION     DAOTEI010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43090_length_2167_cov_3.794034"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1146
                     /locus_tag="P5313_16595"
     CDS             133..1146
                     /locus_tag="P5313_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY82036.1"
                     /translation="MPRPLRVPADPGGRSPPGPLAPEEHARLVSFLRDRTPPVRPLPP
                     PDLPEPFSSLAGRPLPRRPRALILDVYGTLVSSGAGEVGTAAETAESGPDAGPAALAE
                     VLKEFGIREGPSRFEERLLEEIRAEHRAARETGVPWPEVDGAALLARLTGLSLPEARL
                     LGAAREAVLNPAAPMPGAAELLDSARSSGLALGLVSNAQYYTEPALEAAFGRSLDSLG
                     FEPDLRVWSWRLGRAKPDPELFRILARALASRRISPDEAVYVGNDLLNDMAPARAAGF
                     RTALFAGDARSLRLRAGDDRVEGVLPDTVLPTFLAASAVFRLGGRDPFLCVPLGPGGS
                     FRS"
     gene            1254..>2167
                     /locus_tag="P5313_16600"
     CDS             1254..>2167
                     /locus_tag="P5313_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRY82037.1"
                     /translation="MYLTKARDIRRIGFVSTRLQGTDGVSLETRKWDDVLTELGYETF
                     FFSGLSDWYPERSRVVPAAYFLQPEVHALHKEIFRDASRRRGLTADVGEIKGGIKAEL
                     YKFIEDFGVDLIIPENALAIPMHIPLGLAITELIAETEMPTIAHHHDFFWERPRFLTG
                     CAEDYLGRSFPPKLHSIAHVVINTEARQQLAFRCGLSSFMIPNVWDFDEEPVKPDAYN
                     AGFRKDFGLTEDEVIFLQPTRIVARKGIENAIELVARYNKTPEGAGRGRLLISHPELD
                     EGNAYFNRITDYAALLEGYNEAVYDGGI"
BASE COUNT          309 a          795 c          723 g          340 t
ORIGIN      
        1 ggaatactcc ccctaccgct gcggggaccg cctggccgcg gcctacgaac gcacggcggc
       61 ctgtccgggc tccggggcgg ccccggaccg ggaggccctg gcccgggtgc tcctttctcc
      121 ggagggcttc ttttgccccg gcctctgagg gttcccgcgg atcccggcgg caggtccccg
      181 cccggccccc tggccccgga ggagcatgcc cggctggttt ccttcctccg ggaccggacc
      241 ccgcccgtcc ggccccttcc acccccggac ctgccggaac ccttttcgtc cctggccggc
      301 aggcccctgc cccggcggcc ccgggccctg atcctggacg tgtacggaac cctcgtgtcc
      361 agcggcgccg gggaggtggg caccgccgcg gagacggccg agtccggtcc ggatgccggc
      421 ccggcggccc tggcggaggt tctgaaggaa ttcggaatcc gggaggggcc ctcccggttc
      481 gaggagcgcc ttctggagga gatccgggcg gagcaccggg cggcccggga aaccggggtg
      541 ccctggcccg aggtggacgg ggcggccctc ctggcccgcc tgaccggcct gtcccttccg
      601 gaggcgcgac tcctcggggc ggcccgggaa gcggtcctca accccgcggc ccccatgccc
      661 ggggcggcgg aactactgga ttcggcccgc tcgtccggac tggccctggg gctcgtctcg
      721 aacgcccagt actacaccga gccggccctg gaggccgctt tcgggcggag cctggattcc
      781 ctgggtttcg agccggacct ccgggtctgg tcctggcgcc tgggccgggc caaacccgac
      841 ccggagctct tccggatcct ggcccgggct ctcgcaagcc gaaggatctc cccggacgag
      901 gcggtctacg tgggcaacga ccttctgaac gacatggccc ctgcccgggc ggcggggttc
      961 cgcacggccc tgttcgccgg ggacgcccga tccctgaggc tccgggccgg ggacgaccgg
     1021 gtggagggag tccttccgga tacggtgctg ccgacgttcc tggcggcctc cgccgtgttc
     1081 cgactagggg gaagggatcc cttcctctgc gttcccctcg gtccgggcgg cagctttcga
     1141 tcctagaccc tggcacccgc gcccggtacc tccgatagta taagagtcgg aagacttccc
     1201 gggggcgcgg ttcggctcct tccgggcatt ccattcttcc gggaggccca gcgatgtacc
     1261 tgaccaaggc ccgggatatc cggcgtatcg ggttcgtgtc cacccgtctt caggggacgg
     1321 acggagtgtc cctggaaacc cggaaatggg acgacgtgct cacggagctc ggctacgaga
     1381 ccttcttctt ttccggactg tcggactggt accccgagcg ttcccgggtc gtcccggccg
     1441 cgtacttcct gcagcccgaa gtccacgccc tgcacaagga gatcttccgg gacgcctccc
     1501 gacggcgggg cctgaccgcg gacgtcggag agatcaaggg cgggatcaag gcggaactgt
     1561 acaagttcat cgaggacttc ggcgtcgacc tgatcatccc cgagaacgcc ctggccatcc
     1621 ccatgcacat ccccctgggc ctggcgatca cggagctgat cgccgagacg gagatgccca
     1681 ccatcgccca ccaccacgac ttcttctggg aacggccccg gttcctgacc ggctgcgcgg
     1741 aggactacct gggccggtcc ttcccgccca agctgcactc catcgcccac gtggtgatca
     1801 acaccgaggc ccggcagcag ctggccttcc gctgcggact atcctcgttc atgatcccca
     1861 acgtgtggga cttcgacgag gagcccgtga aaccggacgc ctacaacgcc ggcttccgga
     1921 aggatttcgg tctgaccgag gacgaggtca tcttcctcca gcccacccgc atcgtggccc
     1981 ggaagggcat cgagaacgcc atcgaactcg tggcccggta caacaagacc cccgaagggg
     2041 ccgggcgggg caggctcctg atctcccacc ccgagctgga cgaggggaac gcctacttca
     2101 accggatcac ggactacgcc gccctgctgg agggctacaa cgaggccgtc tacgacggtg
     2161 gcatcca
//
LOCUS       DAOTEI010000279         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_44505_length_2125_cov_4.221256, whole genome shotgun sequence.
ACCESSION   DAOTEI010000279 DAOTEI010000000
VERSION     DAOTEI010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44505_length_2125_cov_4.221256"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="P5313_16605"
     CDS             complement(<1..1825)
                     /locus_tag="P5313_16605"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRY82038.1"
                     /translation="MAKKRPKILDARWFGLIIGGMVLGIFLLLNYQTGLLAKLELKVL
                     DTFFILKTTQEKKTLQEGTVQTDRDVKISEDILIVGVDTSSLSRYGNWPFPRRVHADL
                     VNSFARIKNQDNRERSLFLDIFFIDPDRNPANDALLVDSIEKSGRVFLETVLMPSQSL
                     AAEEAGLEVRQEVLYDSWGAIRTIGGDWKSVPGFYGYEPPLEPYGRASRGYGHANFIA
                     DADKIYRRQPLVIKSSVLMEILRLDELGPGYTVNEKEMERLAWQDDRGEFHTIEVPLT
                     AESLASLKAQMAKRAPLKIEDTNQDGTPDAEYHIVRKFKDTFVPAITLSLALDYFGRS
                     LDEIEVILGEHILIPAPRKFDPGSGQWVPYTLVVSREQYDKDGNVVKEAVLREVPEIR
                     IPINEYGQMMVNYMGHRSSESMEGHQTFPVRSYAGYSDKAPGPDPDSWRRTMGVPNKI
                     IMVGAFASGMAEDEKPTPYGLMYGIEIHANALNTILMDNFINKAPDWVDILVVAGMVL
                     VTALLVSRLSALIGVGYTLASVIVYFFVASALFDGSAYLLNYTMPAFATLFTFIAVIV
                     YRALTEEKDKRQMRETFGKYVSPRVVDQLVDDPPELGGEDKE"
     gene            complement(1894..>2125)
                     /locus_tag="P5313_16610"
     CDS             complement(1894..>2125)
                     /locus_tag="P5313_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82039.1"
                     /translation="DTKTAIELWTRVIESSGASVGIPHAHFVLGTLYEETKQYEQAKA
                     QYDKLTAAFPDNDWTKLARNRIILLKSQGLLP"
BASE COUNT          374 a          644 c          674 g          433 t
ORIGIN      
        1 gctccttgtc ctcgccgccg agctcgggcg gatcgtccac gagctggtcc acgacccggg
       61 ggctcacgta cttgccgaag gtctcccgca tctggcgctt gtccttctcc tccgtgaggg
      121 cccgatacac gatcacggct atgaaggtga acagagtcgc gaaggccggc atcgtataat
      181 tgaggaggta cgcggagccg tcgaacaggg ccgaggcgac gaaaaagtac acgatgacgg
      241 aggccagggt gtaccctacc ccgatcagtg ccgagagccg ggagactagg agggccgtga
      301 cgaggaccat gcccgccacg acgaggatgt ccacccagtc cggggccttg ttgatgaaat
      361 tgtccatcag gatcgtgttc agggcgttgg cgtggatctc gatcccgtac atcaggccgt
      421 agggcgtggg cttctcgtcc tcggccatgc cgctggcgaa ggctccgacc attatgatct
      481 tgttggggac acccatggtc cttcgccacg agtccgggtc cggtccggga gccttgtccg
      541 agtagcccgc gtaggaccgg acggggaagg tctggtgccc ttccatgctc tcgctggagc
      601 ggtggcccat gtagttgacc atcatctggc cgtattcgtt gatcggaatc cgtatctccg
      661 gcacctcccg gagcacagcc tccttcacga cattgccgtc cttgtcgtac tgctcccggg
      721 ataccaccag ggtatacggc acccattgac ccgatcccgg atcgaacttc ctgggcgcgg
      781 gaatcaggat gtgctcgccc aggatgacct cgatctcgtc cagggacctc ccgaaatagt
      841 ccagggccag ggagagggtg atggccggaa cgaaggtatc cttgaattta cggacgatgt
      901 ggtactccgc gtccggcgtc ccgtcctggt tcgtatcctc gatcttgagg ggtgcacgtt
      961 tcgccatctg ggccttcaag gacgccaggg attccgcggt cagggggacc tcgatcgtat
     1021 ggaactctcc gcggtcatcc tgccaggcca ggcgctccat ctccttctcg ttcacggtat
     1081 atccgggtcc gagttcgtcg agccgaagta tctccatcag caccgaggac ttgatgacca
     1141 ggggctggcg ccggtagatc ttgtccgcgt cggcgatgaa gttggcgtgt ccgtagcctc
     1201 gggaagcccg cccatagggc tcgaggggcg gctcgtaccc gtagaatccc gggacggact
     1261 tccaatcgcc cccgatggtc cggatggcgc cccagctatc gtacaggacc tcctgccgga
     1321 cctccaggcc cgcctcttcc gcggccaggg actgggacgg cataagcacg gtttccagga
     1381 agacccttcc ggacttctcg atgctgtcca ccaggagggc gtcgttcgcg gggttccggt
     1441 cggggtctat gaagaagata tccaggaaga gggaacgctc ccggttgtcc tggttcttga
     1501 tccgggcgaa ggagttgacc aggtccgcgt gcacccgccg ggggaagggc cagttcccgt
     1561 atcgggaaag ggagctggtg tccacgccga cgatcaggat gtcctcggag atcttgacgt
     1621 cccgatcggt ctgcacggtc ccttcctgca gggttttctt ctcctgggtg gtcttcagga
     1681 tgaagaaggt gtccaggacc ttcagctcca gcttggcaag gaggcccgtc tggtagttca
     1741 ggagcaggaa gatccccagg accatgccgc cgatgatcaa gccgaaccac cgggcatcca
     1801 gtatcttggg acgcttcttc gccataccgg cctcctgctt aataaaacgg cggcccggaa
     1861 atcccgggcc gccggagtta gacctgcctc cgcctacggg agaagtccct gggacttgag
     1921 gaggattata cggttccggg cgagctttgt ccaatcgtta tcggggaagg ccgcggtcaa
     1981 tttgtcgtac tgggccttgg cttgctcgta ctgtttggtc tcctcataga gagttcccag
     2041 tacgaaatgg gcgtggggaa tccccacgga agccccggag ctctcgatca cccgggtcca
     2101 gagctcgatg gccgtcttgg tgtct
//
LOCUS       DAOTEI010000280         2082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_46025_length_2082_cov_5.867292, whole genome shotgun sequence.
ACCESSION   DAOTEI010000280 DAOTEI010000000
VERSION     DAOTEI010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46025_length_2082_cov_5.867292"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="P5313_16615"
     CDS             <1..536
                     /locus_tag="P5313_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY82040.1"
                     /translation="NKIKKNFHNMFRRLFGGGRGELRLVDPDHVLESGIEIYAQPPGK
                     KLEAISLLSGGEKSLTAIALLFATYMVRPSPFCFLDEIDAALDEANVVRFVNLLREFG
                     RISQFIVITHNKKTVIGADALLGVTMEESGVTKVIAVRLERGDEGRAAAPEPEIELED
                     EDVEYESGRELAPDGAS"
     gene            546..1121
                     /locus_tag="P5313_16620"
     CDS             546..1121
                     /locus_tag="P5313_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82041.1"
                     /translation="MNLRELVDRVRKIVSALASRPGGKGRLSAPSGADADGEDGLSGL
                     LGGPSASAPSEFPGFAPPPSLGDRIRSSAADLAGRVRGNPRALLAAGAGLLCVGVAVL
                     AVALVQRPPKPVVPAVSVSPEALSILRRIPVPRPDPLAGESALDRPRKDRYTEEEVDR
                     MWLDLGLLPVDALRERNEAELEALLSAMEKR"
     gene            1118..>2082
                     /locus_tag="P5313_16625"
     CDS             1118..>2082
                     /locus_tag="P5313_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82042.1"
                     /translation="MSTTRRRGVSGPARFLPALLLLAVLATAAACVSAPKEAPEEAPA
                     AAEPKPESAGDPRPEVVEIRGPVPLPVRMVLVLPDPEPKQAIGTASRLPAPQAAPAAS
                     AAAPTLDASAVSPPVAVLVPAAEPPKAAAAPAEKPPEPKPAVPSPPPPQKPAATAAVP
                     KTSTVPAKPAAPAAKPTAPAAGTASPAPAEAPPAARPAPALTPAPGSSARTVPEPAQV
                     PGRTEEAVRGSPLELPFRGSGWVYLGETGGRDGLRFETRKFDGSQAVFLFTAERPGEY
                     QLRFQRQDPFSGEPENLIVKVIVREPEPAAASGTPARPPAGSAAPA"
BASE COUNT          293 a          808 c          699 g          282 t
ORIGIN      
        1 acaacaagat caagaagaac ttccacaaca tgttccgccg cctcttcggc gggggccggg
       61 gagagctccg cctggtggac ccggaccacg tcctggagtc gggcatcgag atctacgccc
      121 agccccccgg gaagaagctg gaggccatca gcctcctgtc cggaggggag aagagcctca
      181 cggccatagc cctcctgttc gccacgtaca tggtccgccc cagccccttc tgcttcctgg
      241 atgagatcga cgcggccctg gacgaggcca acgtggtccg cttcgtgaac ctcctgcggg
      301 agttcggacg gatcagccag ttcatcgtca tcacccacaa caagaagacc gtgatcgggg
      361 cggacgccct cctgggggtc accatggagg agtccggggt caccaaggtc atcgccgtcc
      421 gcctggagcg gggggacgag ggccgcgcgg ccgcgccgga gccggagatc gaactggagg
      481 acgaggacgt ggagtacgag tccggccggg aactggctcc ggacggggct tcctaggacc
      541 gcggaatgaa cctccgggag ctggtcgacc gggtccggaa aatcgtctcg gccctggctt
      601 cccggccggg cgggaagggc cggctttccg ccccgtccgg agcggacgcc gacggggagg
      661 acgggctctc cgggctcctg ggcggaccct ccgcctccgc cccttcggag ttccccggat
      721 tcgccccgcc cccgagcctg ggcgacagga tccggtcttc cgcggccgac ctggccgggc
      781 gggtccgcgg gaacccccgg gccctcctgg ccgcgggagc cgggctcctg tgcgtcggag
      841 tggcggtcct cgccgtcgcc ctggtccaac gtcccccgaa gcccgtcgtc ccggccgtat
      901 ccgtgtcccc cgaagccctc tcgatcctgc ggaggatccc cgtgccccgg ccggatcccc
      961 tggccgggga atccgccctg gatcgcccgc ggaaagaccg atatactgag gaggaggtcg
     1021 accggatgtg gctcgaccta ggcctcctgc cggtggacgc cctgcgggag agaaacgagg
     1081 cggaactcga ggccctcctt tccgcgatgg agaagcgatg agcacgactc ggcgccgcgg
     1141 cgtatccggt ccggcacggt tccttccggc cctcctcctc ctggccgtcc tcgcgacggc
     1201 ggcagcctgc gtaagcgccc cgaaggaagc ccccgaggaa gctccggccg ctgccgaacc
     1261 gaagccggaa tccgccgggg atcctcgtcc cgaggtggtc gagatccggg gaccggtccc
     1321 gcttcccgtc cggatggtcc tggtccttcc ggaccctgaa ccgaagcagg cgatcggcac
     1381 ggcttcccgt cttcccgcgc cgcaggccgc tccggccgcc tccgccgcgg ccccgaccct
     1441 cgacgcttcc gcggtctccc cgccggtggc ggtcctcgtt ccggccgcgg agcctccgaa
     1501 ggcggccgca gccccggccg agaaaccccc ggagccgaag cctgcggtac cttccccgcc
     1561 cccgccccaa aaaccggccg ccacggccgc ggttcccaag acctcgaccg taccggcaaa
     1621 accggccgcc cccgcggcca agcctacggc acccgccgcg ggcacggctt ccccggctcc
     1681 tgcggaagcc ccccctgccg cccggcccgc ccccgccctg acccctgccc cggggtcctc
     1741 ggcccggacg gtcccggaac ccgcccaggt tcccggccgc acggaggagg cggtccgggg
     1801 ttcgcccctg gaactgccct tccggggctc cggctgggtc tacctggggg agacgggagg
     1861 ccgggacgga ctccgtttcg agacccggaa attcgacggc tcccaggccg tcttcctctt
     1921 cacggcggag cgtcccgggg aataccagct ccggttccag aggcaggatc ccttctcggg
     1981 ggagcccgag aatctgatcg tcaaggtgat cgtacgggag cccgaacccg cggccgcctc
     2041 cgggaccccg gcccgccccc ccgcgggttc cgccgctccg gc
//
LOCUS       DAOTEI010000281         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_46266_length_2076_cov_2.835725, whole genome shotgun sequence.
ACCESSION   DAOTEI010000281 DAOTEI010000000
VERSION     DAOTEI010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46266_length_2076_cov_2.835725"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1207
                     /locus_tag="P5313_16630"
     CDS             62..1207
                     /locus_tag="P5313_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY82043.1"
                     /translation="MQYRVFPRIPELPISTLSLGAMRLPVLAGDEARIDPDALDGMLQ
                     AAAEAGINYLDTAYIYHNGASEEAVGRSLDRTGLRGKFHLATKSPTWLVKTESDWEKY
                     LSTQLGRLGTERIDFYLLHALTTARWEKVLRLGGLEFLRKAKADGRIGHIGFSFHDSL
                     DVFKRIVDGWEGWEFCQVQYNYLDTEFQAGREGIRYAASKELGVIVMEPLRGGGLAVL
                     PREAREVFARWPAPRMPAEWALRFALDPQEVVTVLSGMGSESQVWENAAVADAARSNA
                     VTAKETALYEEVRAYFKAKMPVPCTTCGYCLPCPHKIPIPEIFAVYNTWAAFPERRED
                     RRGWYESAYRKSGIGGDACVSCGECRPKCPQGIPIPDRLAEAHGNLS"
     gene            1301..>2076
                     /locus_tag="P5313_16635"
     CDS             1301..>2076
                     /locus_tag="P5313_16635"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRY82044.1"
                     /translation="MSVQKLKPVPSDIDVAQAAEVKPITEIAGRYGIPAERLEMYGEY
                     KAKVRLEFLRENAAAPKKAKYIDVTAITPTPLGEGKTTTTVGLTQGLGLLGRKAICAI
                     RQPSMGPTFGIKGGAAGGGYSQIVPMADFNLHLTGDIHAVSAAHNLCSTALDARIYHE
                     GRWSDSYFEKLGLHKLNFDPYEVWWRRVMDMNDRTLRRILVGVGGIENGPLRETGFDI
                     SVASEVMAILALAKDLKDLRERIGRIVLGLDKSGKQVTTE"
BASE COUNT          372 a          700 c          673 g          331 t
ORIGIN      
        1 cgggctgagg cgggcggggt ccgtattccg catccatgcg gattccgcta tactcccggc
       61 catgcagtac cgagtcttcc ccaggatccc cgagcttccg atctccaccc tgagcctggg
      121 cgccatgcgc ctgcctgtcc tggccggcga cgaggcacgg atcgatcccg atgccctgga
      181 cggcatgctt caggccgccg ccgaggccgg gatcaactac ctggacacag cctatatcta
      241 ccacaacggg gcgagcgagg aggcggtcgg ccgatccctg gaccggaccg ggcttcgggg
      301 gaagttccat ctggccacaa agagtccgac ctggctggtc aagaccgaat cggattggga
      361 gaagtacctc tccacccagc tcgggcgcct ggggacggag cggatcgact tctacctcct
      421 ccacgccctg accacggctc ggtgggagaa ggtcctccgc ctgggaggcc tcgagttcct
      481 gcggaaggcc aaggcggacg gccggatcgg ccacatcggg ttctccttcc acgattccct
      541 ggacgtgttc aagcggatcg tggacgggtg ggagggctgg gagttctgcc aggtccagta
      601 caattacctg gataccgagt tccaggccgg ccgggagggc atccgctacg cggcctccaa
      661 ggaactcggt gtcatcgtca tggagcccct gcggggaggc ggcttggcgg tcctcccgcg
      721 ggaggcccgg gaagtcttcg cccgctggcc cgcgccccgg atgcccgcgg agtgggccct
      781 ccgcttcgcc ctggaccccc aggaggtggt taccgtcctg tcgggcatgg gctccgagtc
      841 ccaggtctgg gagaacgcgg cggtcgcgga cgcggcccgg tccaacgcgg tcaccgcgaa
      901 ggagacggcc ctctacgagg aagtccgcgc ctatttcaag gccaagatgc ccgtaccctg
      961 cacgacctgc gggtactgcc tgccctgtcc gcacaagatt ccgatccccg agatcttcgc
     1021 cgtctacaac acctgggccg ccttccccga acgccgggag gaccgcaggg ggtggtacga
     1081 gagcgcctac cgcaagtccg gcatcggggg agacgcttgc gtctcctgcg gggaatgccg
     1141 gcccaagtgt ccccagggta ttccgatccc ggaccgactg gcggaggccc acggaaacct
     1201 ctcgtgaagc gccgctattc ctaattgggt atgaatatat ttaattggac acggatgccc
     1261 cggcgggtta ctttttaggt accctgaaca aggaggcccc atgtccgtcc agaaactcaa
     1321 acccgttccc agcgacatcg atgtagccca agccgcggaa gtgaagccca taacggagat
     1381 cgccggccgc tacggcatcc ccgccgagcg cctggagatg tacggggagt acaaggccaa
     1441 ggtacgcctg gagttcctgc gggagaacgc ggcggctccc aagaaggcca agtatatcga
     1501 cgtgaccgcc atcaccccca cccccctggg ggagggcaag accacgacca ccgtgggtct
     1561 gacccagggt ctgggcctcc tgggccgaaa ggcgatctgc gctatccgcc agccctccat
     1621 ggggccgacc ttcgggatca aggggggagc cgcgggcggg ggctacagcc agatcgtccc
     1681 catggcggac ttcaacctcc acctgaccgg ggacatccac gcggtgtccg ctgcccacaa
     1741 cctctgctcc accgccctgg acgcccggat ctaccacgag ggccgctggt ccgattccta
     1801 cttcgagaag ctggggctcc acaagctcaa cttcgatccc tacgaggtct ggtggcgtcg
     1861 ggtcatggac atgaacgacc gtaccctgcg caggatcctg gtcggagtcg ggggcatcga
     1921 aaacggcccc ctccgggaga cgggcttcga catctccgtg gccagcgagg tcatggccat
     1981 cctggccctg gccaaggacc tcaaggacct cagggagcgg atcggacgca tcgtcctggg
     2041 cctggacaag tccggcaagc aggtgaccac cgagga
//
LOCUS       DAOTEI010000282         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_48386_length_2022_cov_4.316726, whole genome shotgun sequence.
ACCESSION   DAOTEI010000282 DAOTEI010000000
VERSION     DAOTEI010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48386_length_2022_cov_4.316726"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1283
                     /locus_tag="P5313_16640"
     CDS             264..1283
                     /locus_tag="P5313_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRY82045.1"
                     /translation="MVTILPIASGKGGVGKTVLAANLGVCLAGLGRTVVLADLDLGGA
                     NLHTCLGVKNRNPGIGALAWRQEKSLANLLVETRVDRLWFIPGDNLLPGTANLEFFVK
                     QRILRELARLPADFVLLDLGAGSSYNVVDFFLTSSSGLLVIQPEATSILNAYSFLKTS
                     VYRMLFRSFPRASEERRRVADFVSRRVEGSGASFLELARELARDFPASAPPALEQLSR
                     FFPRVVLNEGRGPKDASLGLHLRDMVTRNLGIPMEYVGFLLRDDAVPRSVAERTPLAL
                     SRPGSPFARGAAALAARIAAQPGGAPPRLFEDDEDLSGALEEAFRDQDYPVEEGPGEG
                     SAGGP"
     gene            1280..>2022
                     /locus_tag="P5313_16645"
     CDS             1280..>2022
                     /locus_tag="P5313_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRY82046.1"
                     /translation="MRPGSSPTAAVFASARAAVLVLATLVSACLPGDPSEPAAVSSDP
                     WAGHAERARVLAGALSLEAACGQLVMTGIGGRGSLDQPSRRLLEELPAGAVILFGFNI
                     PPPAGDLRPALDLAQEIARGTGAGIPLFIAVDHEGGDVFRFRDGVTRLPSARTQGEAG
                     PAAVSRAGAVAGRELKALGINLNLAPVAEAWDARNGGFLGTRAFSADPEEAGALAAAF
                     LAAQQAEGTAAAAKHYPGNGSEDPHKAVPV"
BASE COUNT          250 a          742 c          718 g          312 t
ORIGIN      
        1 ccaggatcag gaaggcggaa ccctccgtct gggtgtccgt cacctcgatg accggggggg
       61 gatcgggggt gtaggcccgg cagggcaggg cggccagcag gaccgcggcc aggcccaggg
      121 cgcgggggag gatcttggac atggatcgag tgtatccccc cgggccctcc gcttcaaggc
      181 gctccgtctt gccggaggcc ggcgcttcct gtacagtcag ggtatcgcgt tgtccgactc
      241 gagcgcggaa accacggagt agcatggtca cgatacttcc gatcgcctcc ggcaaggggg
      301 gcgtgggcaa gacggtcctc gcggcgaacc tgggggtttg cctggccggc ctgggcagga
      361 cggtcgtgct ggcggacctg gacctggggg gcgcgaacct gcacacctgc ctgggcgtga
      421 agaaccgcaa cccgggcatc ggggccctgg cctggaggca ggagaaatcc ctggccaacc
      481 tcctggtgga gacccgggtg gatcggctct ggttcatccc cggggataac ctcctgcccg
      541 gcacggccaa cctcgagttc ttcgtgaagc agaggatcct ccgggaactc gcccgccttc
      601 ccgcggattt cgtcctcctg gacctgggag ccggctcctc ctacaacgtc gtggacttct
      661 tcctgacctc ctcctcgggc ctgctggtca tccagccgga ggcgacctcg atcctgaacg
      721 cctattcctt cctgaaaact tcggtctacc ggatgctgtt ccggtccttc ccccgggcca
      781 gcgaggagcg ccgccgggtc gcggacttcg tgtcccggcg cgtggaaggc tccggagcct
      841 ccttcctgga gctggctcgg gagctcgcgc gtgattttcc cgcctccgcc cctccggccc
      901 tggagcagct ttcccggttc ttcccccggg tggtcctgaa cgagggccgg ggtcccaagg
      961 acgcctccct ggggctccac ctgcgggata tggtcacgcg caacctgggc atcccgatgg
     1021 agtacgtcgg cttcctcctc cgggacgatg cggtgccccg ctcggtggcc gagcgcaccc
     1081 ccctggccct gtcccgcccc ggatccccct tcgcccgcgg ggccgcggcc ctggccgccc
     1141 ggatcgccgc ccagcccggg ggagcgcctc cccggctctt cgaggacgac gaggatctct
     1201 cgggcgccct ggaggaagcc ttccgcgatc aggactaccc ggtcgaggaa ggccccggcg
     1261 agggctccgc gggcggccca tgaggcccgg ttcgagtccg accgcagcgg tcttcgcatc
     1321 cgcccgggcg gcggtcctgg tcctggcgac cctggtttcc gcctgcctgc ccggcgatcc
     1381 ctcggagccc gcggccgtct cctccgatcc ctgggccggg catgccgagc gggcccgggt
     1441 gttggccggg gccctgagcc tggaggccgc ctgcggacag ctggtcatga ccgggatcgg
     1501 aggccggggc tccctggacc agccgtcccg caggctcctc gaggagctcc ccgcgggcgc
     1561 ggtgatcctc ttcgggttca acatcccccc cccggccggg gacctgcggc ccgccctgga
     1621 cctggcccag gagatcgccc ggggaaccgg ggcggggata cccctgttca tcgcggtgga
     1681 ccacgagggg ggcgacgtct tccggttccg ggacggggtg acccggctgc cctccgcccg
     1741 gacccagggt gaggcgggcc ccgcggccgt ttcccgggcc ggggccgtag ccgggcggga
     1801 gctcaaggcc ctgggaatca acctcaacct cgcccccgtt gcggaggcct gggacgcccg
     1861 gaacgggggt ttcctgggca cccgggcctt ctccgcggat cccgaggagg ccggcgccct
     1921 ggcggcggcc ttcctcgcgg cccagcaggc cgagggcacc gcggccgccg ccaagcacta
     1981 tcccggaaac ggttcggagg atccccacaa ggccgtgccg gt
//
LOCUS       DAOTEI010000283         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_50828_length_1964_cov_3.688318, whole genome shotgun sequence.
ACCESSION   DAOTEI010000283 DAOTEI010000000
VERSION     DAOTEI010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50828_length_1964_cov_3.688318"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="P5313_16650"
     CDS             complement(<1..654)
                     /locus_tag="P5313_16650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY82047.1"
                     /translation="MPRNERLGILSLLAAAFLFALTTALVKLSSASLSGYLNSAMRFL
                     VGIVLSAAAILTRSGKIRVTNRKDWVLRGLYGAVSMILSYLAIARTSGGRATLLGNTY
                     PIFVALFAALFFRERLSRAAVPSLALCTLGSALVLNDGAGYPTTGDVLAILSAVFAGL
                     ALNHLKRARATEDPFTLYLSPCLFGLPVSAVLSARGVAFDPGALALAAGIGVVVFLAQ
                     "
     gene            779..1114
                     /locus_tag="P5313_16655"
     CDS             779..1114
                     /locus_tag="P5313_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRY82048.1"
                     /translation="MEEISYGDFKKLDIRVGRVLSAERVPNSEKLYKVQIDAGLDRPR
                     QTVTSLVDYYTAEELVGKTVVVLVNLAPARMRGEVSECMLLAAETPDAAACVLLTPEK
                     DIAPGTTIV"
     gene            1173..>1964
                     /locus_tag="P5313_16660"
     CDS             1173..>1964
                     /locus_tag="P5313_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82049.1"
                     /translation="MRNPARPLSAATLVLVLLTLPAALSPAQEAEDSLSLNIVLGYGP
                     PNLDGYGSTGFAPIDFDIIDAPDANSRDLGTGWGGGVALATLEYSRTIPAFVGEGPFF
                     SGNNLELSARAELSPVTVEAVGSATLTPIAFLKFQAGAAVGTGWTLGFVGLALRPTAP
                     GEMEEIPFGGALVRAWLSGTFQFDLAALLPGDWNHVVVQAVAKFEYRALSSADPGQAW
                     VWQADAGLNFNGWRHLGTYVLGYQMPRKLNFAGVMVETEAWLGDLI"
BASE COUNT          340 a          630 c          696 g          298 t
ORIGIN      
        1 ctgggccagg aagaccacga cgccgatccc ggcggccagg gccagtgcgc cggggtcgaa
       61 ggcgaccccc cgggccgaga ggaccgcgga aacgggaaga ccgaagaggc agggcgacag
      121 gtagagtgtg aagggatcct ccgtggcccg ggcccgcttg aggtggttca gggcgagccc
      181 ggcgaagacc gcggacagga tggcaaggac gtccccggtg gtaggatacc cggccccgtc
      241 gttcaggacc agggcgctcc ccagggtgca gagggccagg ctggggaccg cggcgcggct
      301 gaggcgctcc cgaaagaaga gggcggcgaa gagggccacg aagatcgggt aggtgttccc
      361 taggagggtc gccctccctc ccgaggttcg ggcgatggcg aggtacgaga ggatcatgga
      421 gacggcgccg tagaggcccc gcaggaccca gtccttccga ttcgtcaccc ggatcttccc
      481 ggaccgggtc aggatcgcgg cggcgctgag gacgatgccc accaggaacc gcatcgcgga
      541 gtttaggtac ccggacaggg aggcggagga caacttgacc agggcggtgg taagggcgaa
      601 gagaaaggcc gcggccagga gagaaagaat tccgagtcgt tcgttccggg gcatgggatg
      661 ggtatgccat ggccggcctc cttcgggcaa gaccgggtga ctcccatcgt accccgggtc
      721 cggttttccc gaattccgga ttgcgggcta cgttgctcgc cgctatactc tggagcttat
      781 ggaagagatc agctacgggg atttcaagaa gctggacatc cgggtcggcc gtgtcctatc
      841 cgccgaacgg gtcccgaact cggaaaagct ctacaaggtt cagatcgacg cgggcctgga
      901 ccggccccgg cagaccgtca ccagcctggt ggactactac accgcggagg aactggtcgg
      961 aaagaccgtg gtggtcctgg tcaacctggc ccccgcccgg atgcgggggg aggtttcgga
     1021 atgcatgctc ctggccgcgg aaactccgga cgcagcggcc tgcgtattgc tgacgcctga
     1081 aaaggacatc gctccgggaa cgaccatcgt ctgagccccg gccggcgccc cgatgtgccg
     1141 gccgtcaagt tgcatcccgt acggaggaac ccatgcgcaa tcccgcccgc cccctgtccg
     1201 ccgccaccct tgtcctcgtt ctgctgaccc tgcccgccgc cttgtcgccg gcccaggaag
     1261 ccgaggacag cctcagcctg aacatcgtac tgggctacgg tcctcccaac ctggacgggt
     1321 acgggtccac gggcttcgct cccatcgatt tcgacatcat cgacgcgccg gatgcgaact
     1381 cccgcgacct gggaacgggc tggggcggag gcgtcgccct ggcgaccctg gagtactccc
     1441 gcacgatccc ggccttcgtc ggggaggggc ccttcttctc cgggaacaac ctggaactct
     1501 cggcccgggc ggaactgagc cccgtcacgg tcgaggccgt gggatccgcg accctgacgc
     1561 cgatcgcctt cctcaagttc caggccggcg ccgcggtcgg cacgggctgg accttgggct
     1621 tcgtagggct ggcgctgagg ccgacagcac ccggggaaat ggaggaaatc cccttcgggg
     1681 gcgccctggt ccgggcctgg ctttccggga ccttccagtt cgatctggcg gcgctcctgc
     1741 ccggggactg gaaccacgtg gtggtgcagg cggtcgcaaa gttcgagtac cgcgccctga
     1801 gctccgcgga tcccggccag gcctgggtat ggcaggcgga cgcgggcctt aacttcaacg
     1861 gctggcgtca cctggggacc tacgtcctgg gctaccagat gccccgaaag ctgaacttcg
     1921 cgggggtgat ggtggagacc gaagcttggc tgggagacct gatc
//
LOCUS       DAOTEI010000284         1926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_52481_length_1926_cov_4.736505, whole genome shotgun sequence.
ACCESSION   DAOTEI010000284 DAOTEI010000000
VERSION     DAOTEI010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52481_length_1926_cov_4.736505"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="P5313_16665"
     CDS             <1..910
                     /locus_tag="P5313_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRY82050.1"
                     /translation="TAQSHNYYPVAYLKPEDGLCNAALGLGRWVVEGGDTFRFAPGYP
                     KLEIVAQEHRVANSQRRFIAIDLDRASPELLSGEDAGLAELDIEEVEGTPEFPLVAST
                     FDLADRRLVPGISARGPRILDFANILQHEALPFAPAVEAVLEVGSNSMGIPIEIEYAV
                     NLAETSGVPALYLLQIKPLIQNANKVAVDLGEVDREDCVLTTDRAMGNGRDVSIRDLV
                     FVVPETFDPEATQEIAQEIAQLNDRLKEEKRKYILIGPGRWGPGIGAWAYPSASCRSP
                     GPGSSWRRTWRASGWRAPWAPTSSTT"
     gene            817..1143
                     /locus_tag="P5313_16670"
     CDS             817..1143
                     /locus_tag="P5313_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82051.1"
                     /translation="MQISGARVIVEADLEGFRVEGSLGSHFFHNVTSMNIGYFTVPWD
                     SRSAFVNWEYLQTRPVERRTPHCVHLRFEEPLEILMDGRKSSATVKKSAAALTAGPDS
                     DNIACE"
     gene            1192..>1926
                     /gene="pheS"
                     /locus_tag="P5313_16675"
     CDS             1192..>1926
                     /gene="pheS"
                     /locus_tag="P5313_16675"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 2 subfamily; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRY82052.1"
                     /translation="MDTKSLVRTMHPLEIKVLGAYVPGRELSAALLAADLGYKEGQAN
                     QAFSWLSAKGLAEEAGRTVRTVYELTPLGLEWITAGTPEERILRHLTDKGPARLPEIC
                     AALGLEQKDVGSAFGLLSKEGVLSMDAEKRAVADPAWFETAHASGRKPRIARVREVLE
                     RARAAEGGILEESSLSPEERAAVAGIARKRGAGDAAFRVSDRESVAYRLTEAAGEVRK
                     ELAEAGITGDEEGALTPEMLETGSWKN"
BASE COUNT          337 a          661 c          654 g          274 t
ORIGIN      
        1 caccgcccag tcccacaact actatcccgt ggcctacctc aagcccgagg acggactgtg
       61 caacgccgcc ctgggcctgg ggcgctgggt ggtggagggg ggggacacct tccgcttcgc
      121 cccggggtac cccaagctgg agatcgtggc ccaggagcac cgggtggcca actcccagcg
      181 cagattcata gccatcgacc tggaccgggc ctccccggag ctcctctcgg gggaagacgc
      241 gggcctcgcg gaactggaca tcgaggaggt ggaggggacc cccgagttcc ccctggtggc
      301 ctcaaccttc gacctggcgg accgccgatt ggttccgggg atttccgccc ggggtccccg
      361 gatcctggac ttcgccaaca tcctccagca cgaggcactt cccttcgcac cggcggtgga
      421 ggccgtcctg gaggtgggct ccaacagcat gggaatcccc atcgagatcg agtacgccgt
      481 aaaccttgcg gagacctcgg gcgtcccggc cctctacctc ctgcagatca agcccctgat
      541 ccagaacgcg aacaaggtgg ccgtggatct cggggaggtg gaccgggagg actgcgtcct
      601 gaccacggac cgggccatgg gaaacggccg ggacgtctcc atccgggacc tcgtgttcgt
      661 ggttcccgag accttcgatc ccgaggccac ccaggagatc gcccaggaga tcgcccagct
      721 aaacgaccgg ctcaaggaag agaaacggaa gtacatcctg atcggacccg gccgctgggg
      781 acccgggatc ggcgcctggg catacccgtc agcttcatgc agatctccgg ggcccgggtc
      841 atcgtggagg cggacctgga gggcttccgg gtggagggct ccctgggctc ccacttcttc
      901 cacaacgtga cctccatgaa catcggctat ttcacggtgc cctgggactc ccggagcgct
      961 ttcgtgaact gggaatacct ccaaacccgg cccgtcgagc gccggacccc ccactgcgtc
     1021 cacctgcgct tcgaggaacc cctcgagatc ctcatggacg ggcggaaatc cagcgcgacg
     1081 gtcaaaaaat cggcggccgc attgaccgcc ggacccgatt ccgacaatat agcctgcgaa
     1141 taacggtaca ccaccggaac gcccctcggg gcccgacgat cggagcacag tatggatacc
     1201 aagtccctgg tgcgcaccat gcaccccctc gaaatcaagg tcctgggagc ctatgtcccg
     1261 ggccgggagc tctccgcggc gcttttggcc gccgacctcg gatacaagga gggccaggcc
     1321 aaccaggcct tcagctggct ctccgccaag ggcctggccg aggaggccgg acggacggtc
     1381 cggacggtct acgagctgac ccccctgggg ctcgagtgga tcacagcggg aacccccgag
     1441 gagcgcatac tccgccacct gacggacaag ggaccggccc ggttgccgga gatctgtgcc
     1501 gccctgggcc tcgagcagaa ggacgtcggc tccgccttcg gcctcttgtc caaggagggg
     1561 gtcctctcca tggacgccga gaagcgggcc gtggccgatc cggcgtggtt cgaaacggcc
     1621 cacgcctccg gccggaagcc ccggatcgcc cgggtgcggg aggtcctgga gcgggccagg
     1681 gccgcggagg gagggatcct ggaggaatcc tccctttccc cggaggagcg cgccgcggtc
     1741 gccggcatcg cccgcaagcg gggcgccggg gacgcggcct tccgggtctc ggaccgggag
     1801 tccgtggcct accgcctgac cgaggcggcc ggcgaggtcc ggaaggagct cgcggaggcc
     1861 gggataacgg gggacgagga gggggccctg accccggaga tgctcgagac gggatcctgg
     1921 aagaac
//
LOCUS       DAOTEI010000285         1912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_53139_length_1912_cov_4.092084, whole genome shotgun sequence.
ACCESSION   DAOTEI010000285 DAOTEI010000000
VERSION     DAOTEI010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53139_length_1912_cov_4.092084"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="P5313_16680"
     CDS             <1..731
                     /locus_tag="P5313_16680"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRY82053.1"
                     /translation="LDEVYSRSPANYAAGFPLHPHRGIDTVTYMLSGFLRQIDSLGGA
                     CMIGPGCLQWMTAGSGVIHEEMPLESAVLRGFQLWVNLPRAGKMVDPEYREAAPGEIP
                     IVPILAGKVRVLAGRFGGASGPVVGIQGHPGFLDVELDPGAVFVAEVPEGRKALVHVF
                     EGALAEVGTQRALNPPDPGVLLLRDGDSVRFRAGTQGARFLLLTGAPLGEPIAWKGPV
                     VMNEMEEVDRAYREFGTGTFVKVR"
     gene            complement(764..1852)
                     /locus_tag="P5313_16685"
     CDS             complement(764..1852)
                     /locus_tag="P5313_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRY82054.1"
                     /translation="MSAEKPEDGPDSSVLLPAGPADCDAGAGTFLQTGFWGAFKSEFG
                     WKPRHFRTGRDGAPLLVLERTLTRGFSFAYIPRGPSAGADNTSAPEGLGGMAEALRSH
                     LSADCVFLRVDPSWYAEGGEASIPPPAPPFPFRRAGSDVQVPDTVILDLRGRSDEELL
                     SRMKPKWRYNIKYAEKKGVEVASEPGPGAVEVFYSLYRATALRDRIALHPERYYARLV
                     SLAGDYGPGAPDIRVWIARYRGTPLAAIITSYLRGEAVYLYGASGDEHRNLMPAYALQ
                     WAAIRAARDAGCGYYDFFGIPPSDDPGHPMAGLYRFKTGFGGGIRRYPGSWDYPLRRF
                     PYGAYRGAEGLRGFWFKTVRKRLGGAGR"
BASE COUNT          312 a          648 c          652 g          300 t
ORIGIN      
        1 tcctcgacga ggtctactcc cggtcacccg ccaattatgc ggcgggcttc cctctccacc
       61 cccaccgggg aatcgacacg gtcacctaca tgctctcggg cttcctccgg cagatcgact
      121 ccctgggcgg cgcgtgcatg atcggtcccg ggtgcctcca gtggatgacc gcgggttccg
      181 gggtcatcca cgaggagatg cccctggaat ccgccgtgct ccggggattc cagctttggg
      241 tgaacctgcc ccgggccggc aagatggtcg atccggagta ccgggaggcc gcccccgggg
      301 aaataccgat cgttcccatc ctcgccggaa aggtacgggt actggccggt cggttcgggg
      361 gagcatccgg gccggtcgtc ggaatccagg gccacccggg attcctggac gtggagctgg
      421 atcccggagc ggtcttcgtc gcggaggttc cggaaggacg gaaagccctg gtccacgtct
      481 tcgagggcgc cctggcggaa gtgggaactc aacgggcctt aaaccctccg gacccgggcg
      541 ttctattgct gcgggacggc gactccgtac gattccgggc ggggacgcag ggcgcgcgat
      601 tcctcttgtt gaccggggca cccctggggg agccgatcgc ctggaaggga cctgtggtca
      661 tgaacgagat ggaggaggtc gaccgggcgt accgtgaatt cggcacgggc accttcgtga
      721 aagtccgctg acggccttcc ggaccgaagg cctccggcca agctcagcgc cccgcgcctc
      781 ccagacgttt ccgtacggtc ttgaaccaga atccccgcaa tccttccgca ccccggtagg
      841 ctccgtaggg aaaccgccgc aggggataat cccaggaacc ggggtaccgg cgtatgccgc
      901 ctccgaagcc cgtcttgaac ctgtacaggc cggccatggg atgccccgga tcgtccgagg
      961 gcgggattcc gaagaaatcg tagtacccgc accccgcgtc ccgggccgcc cggatggccg
     1021 cccattgaag ggcgtaggcg ggcatgaggt tccggtgttc gtccccggac gcgccataga
     1081 ggtagacggc ctccccccgc aggtaggagg taatgatggc ggccaggggg gttccgcggt
     1141 accgggcgat ccaaacccgg atgtccggcg ccccgggtcc gtagtccccg gccagggata
     1201 ccaggcgcgc gtaatatcgt tcgggatgga gggcgatccg gtcccgcagg gccgtggccc
     1261 ggtacaggga gtaaaacacc tccaccgccc cgggacccgg ctccgacgcg acctcgaccc
     1321 ccttcttctc ggcgtacttg atgttgtacc gccacttggg cttcatacgg gagagcagtt
     1381 cctcgtcgga cctgccgcgc aggtccagga ttacggtatc cggaacctgc acgtcggaac
     1441 cggccctgcg gaacgggaag ggcggcgccg ggggagggat cgacgcctcg ccgccctcgg
     1501 cgtaccagga cgggtccacc cgaaggaaaa cgcagtccgc ggagagatgg gaccggaggg
     1561 cttccgccat ccctccgagc ccctccggtg ccgaggtgtt gtccgccccc gcggacgggc
     1621 cgcgggggat gtacgcgaag gagaagcccc gtgtcagagt ccgctccagg acgaggaggg
     1681 gcgcaccgtc ccggcccgtc cggaaatgcc ggggtttcca tccgaactcg gatttaaaag
     1741 ccccccagaa ccccgtctgt aggaaggtcc cggctcccgc gtcgcagtcc gccgggcccg
     1801 cgggaaggag gacggaagag tccggaccgt cctccggctt ttcagccgac attcccgtcc
     1861 gcgacccgta ccgtcgacag ggctcggccc tcggcctgga gagtcttcgt tc
//
LOCUS       DAOTEI010000286         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_55695_length_1861_cov_3.628461, whole genome shotgun sequence.
ACCESSION   DAOTEI010000286 DAOTEI010000000
VERSION     DAOTEI010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55695_length_1861_cov_3.628461"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="P5313_16690"
     CDS             <1..803
                     /locus_tag="P5313_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82055.1"
                     /translation="FVHIDRDVSVFRLSDDLEGEEAPIPADEGISTLAEEGVAPLTAP
                     PEDTSYLEDAEELELAGDEDLPEAPLLEPSPEELNIDELVEEGFEEAEELPLAEFESA
                     EGLEEAADLEEESEELEELTLDLDSEPSSIVLESPENIELPIPEIEETEPGSMESAPE
                     FVEELASEEDSAGLGEIALHHEESPVPVSAGSPGIAEPEALLVEDLEPGDTPKPEQIP
                     NRIKDEVRSVLTYLDKLLEALPEEKIEEFAQSEHYETYKRLFEELGLV"
     gene            1226..1726
                     /locus_tag="P5313_16695"
     CDS             1226..1726
                     /locus_tag="P5313_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82056.1"
                     /translation="MLSLLGASLAAPLKASRPPCLRHDLPPERPPSELAAAASTRFAD
                     SQAASATLCVLDPRPFLDSAAESFPQLNPECLAEDLARCLAAYFESSGGLFLCDSGEL
                     AALCCGSRPVDTGLLQSQLGKYVRKFISPAVEVPIRVRRSRTFESPMTGEMESFLAEC
                     SDGPGA"
BASE COUNT          322 a          623 c          588 g          328 t
ORIGIN      
        1 tttttgtaca tatcgaccgc gacgtttccg ttttccgcct ctccgacgat ctcgagggcg
       61 aagaggcccc gataccggcg gacgagggca tatccacgtt ggctgaggag ggagtggctc
      121 ccctcaccgc tccccccgag gatacctcct atcttgagga tgccgaggaa ctggagctcg
      181 caggcgacga ggatctcccg gaggcccccc tgctggagcc gtccccggaa gagctgaaca
      241 tcgacgaact cgtggaagag ggattcgagg aggccgagga actaccgttg gcggaattcg
      301 agtccgccga gggtctggag gaagccgcgg acctcgaaga ggaatcggag gagctggagg
      361 agctcaccct cgacctggac agcgaaccct cctccatcgt cctggaatct ccggagaaca
      421 tcgaactgcc gataccggaa atcgaggaaa cggagcccgg atccatggag tccgctccgg
      481 agttcgtgga ggaactggcc tccgaggaag actccgccgg actcggggag atcgccctgc
      541 accacgagga atcccccgtg ccggtatccg cgggcagccc cgggatcgcg gagcccgagg
      601 ccctgctggt cgaggacctg gagcccgggg atacccccaa gccggagcag atcccgaatc
      661 ggatcaagga cgaggttcgc agcgtgctca cgtacctgga caagcttctg gaagccctcc
      721 cggaggagaa gatcgaggag ttcgcccagt ccgaacacta cgagacgtac aagaggctct
      781 tcgaagagct ggggttggtg taaggtatgg gtctactgga caaggcatcc cgcgggtttt
      841 cccggcccgc cccggcccgc ggcggcgggc ttcttgagag ggcatcccgg acgcgcaccg
      901 tccccggggg agaaaaaaaa aactaagcag ccttgatgcc gacgaggctc tgggcgccat
      961 cctgtcggaa tgtctcgagg ccccggcgga ttactttctt cccgcccgga tcctccggat
     1021 tctccgggac cgcggggggt tccccgactt aaggatcacc ctggaagaag gctcggaagc
     1081 cggttcgagg gttttcgcgt ccatcctgga ctccggcgaa cactccgccg gctatgacgc
     1141 cgcaggtgag gattccgccg gttcgagctg tctcctgggc cctcattacc gccttgaggt
     1201 actcggagtc accggggcgg agcggatgct ctccctcctg ggcgcttccc tggccgctcc
     1261 cctgaaagcc tcccgaccgc cctgtctccg ccacgacctt ccgccggaac gacccccttc
     1321 ggaacttgcc gcggcggctt ccactcgatt cgcggattcc caggcggcga gcgccaccct
     1381 ctgcgtcctg gatcccaggc ccttccttga ttccgcggcg gagtccttcc cccagctcaa
     1441 tcccgagtgc ctcgcggagg atctggcccg ctgcctggcc gcctacttcg agtcgtcggg
     1501 gggtctgttc ctctgcgatt ccggggaact ggcggctctt tgctgcggat cccgccccgt
     1561 ggacacggga ttgctccaga gtcaactggg caagtatgtg cggaaattca tctctccggc
     1621 cgtagaggtt cccatacgcg tacgcaggtc ccggaccttc gaatctccca tgaccggtga
     1681 aatggaatcc ttccttgccg agtgctccga cggacccggc gcgtgaaccc aggctgatcc
     1741 ccggtacgat ggccgatccg atcgtctccc cgcgcctgca ttcccggttc gatcccgcgg
     1801 gggaggcggc acggtacctc gaggaatccc tgggaggccg ccgcccgtcc tgcctgctcg
     1861 t
//
LOCUS       DAOTEI010000287         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_55949_length_1856_cov_4.270405, whole genome shotgun sequence.
ACCESSION   DAOTEI010000287 DAOTEI010000000
VERSION     DAOTEI010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55949_length_1856_cov_4.270405"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="P5313_16700"
     CDS             complement(<1..241)
                     /locus_tag="P5313_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRY82057.1"
                     /translation="MAEPTGKTIPVSIDDEVKTAYLNYAMSVIVSRALPDARDGLKPV
                     HRRLLFAMDELGLRPNAATKKSARITGDAMGKYHPH"
     gene            complement(267..>1856)
                     /locus_tag="P5313_16705"
     CDS             complement(267..>1856)
                     /locus_tag="P5313_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRY82058.1"
                     /translation="SYKVSGGLHGVGVSVVNALSSVCEAFVHLNGSIHFQRYRMGIPE
                     APVSILGTTEKRGTVVRFQPDPSIFESTVFSFDVLAGRLRELAFLNKGVTITLTDERL
                     STPKTHEFRFDGGLRQFVEYLNKNKTVLHKEPIYFSGTRDDVEIEVGLQYNDTFNETM
                     FAFVNGINTREGGTHLIGFKSSLTRILNEYLKKSKLAKKMEDTLSGDDVREGLVAVLS
                     VKVQNPQFEGQTKGKLGNSEVKGIVESFCNDQFTLYFETYPEVINAILEKTVLAAKAR
                     IAARQARELTRRKSFLESAGLPGKLADCSEKDPAKCEIFIVEGDSAGGSAKMGRDRSF
                     QAILPLWGKMLNVEKTRIDKVIGNDKLQPIIASLGTGIGEDYDVSKLRYHKVIIMADA
                     DVDGSHIRTLLLTFFYRYMTEMLEKGHVYLAMPPLYKIAFDKQVHYAYDDAEKDRILA
                     SVGKDPEKVNVQRYKGLGEMNPDQLWETTMDPGRRSIMQIRMEDAVEAEEVFTTLMGE
                     QVEPRRKFIEENAVYVRNLDV"
BASE COUNT          287 a          564 c          622 g          383 t
ORIGIN      
        1 cgtgggggtg gtacttaccc atggcgtcgc cggtgatgcg ggcgctcttc ttggtcgcgg
       61 cgttgggcct gagtcccagc tcgtccatgg cgaagaggag cctgcggtgc acgggcttga
      121 ggccgtcccg ggcgtccggc agggcgcggc tcacgatgac gctcatcgcg tagttcaggt
      181 aggcggtctt gacctcgtcg tcgatggaga cggggatcgt cttgccggtc ggttcagcca
      241 tggaagacag ctcctgggtt ccgggatcag acgtccaagt tccggacgta tacggcgttt
      301 tcctcgatga acttccgccg gggctccacc tgctcgccca tcagggtggt gaagacctcc
      361 tcggcctcca ccgcgtcctc catgcggatc tgcatgatgc tgcggcggcc cgggtccatg
      421 gtggtctccc agagctggtc ggggttcatc tcgcccagac ccttgtagcg ctggacgttg
      481 accttctcgg gatccttgcc gacggaggcc aggatccggt ccttctcggc gtcgtcgtag
      541 gcgtagtgaa cctgcttgtc gaaggcgatc ttatagaggg gcggcatggc caggtagacg
      601 tggccctttt ccagcatctc cgtcatgtag cggtagaaga aggtcaaaag gagggtccgg
      661 atgtgggacc cgtccacgtc ggcgtccgcc atgatgatga ccttgtggta ccggagcttg
      721 gagacgtcgt agtcctcccc gatccccgtg cccagggagg cgatgatggg ctggagcttg
      781 tcgttcccga tgaccttgtc gatccgggtc ttctccacgt tcaacatctt gccccagagg
      841 gggaggatgg cctggaagct ccggtcccgg cccatcttgg ccgagccgcc ggcggaatcc
      901 ccctccacga tgaagatttc gcacttggcg gggtccttct cggagcagtc cgccagcttg
      961 ccgggcaggc ccgcgctctc caggaaggac ttgcgccggg tcagctcccg ggcctgccgc
     1021 gccgcgatgc gggccttggc ggccaggacg gtcttttcca gtatcgcgtt gatgacctcg
     1081 ggataggtct cgaagtagag ggtgaactgg tcgttgcaga aggactccac gatccccttc
     1141 acctcggagt tgcccagctt gcccttggtc tgcccctcga actggggatt ctggaccttc
     1201 acggagagga ccgccaccag gccttcccgg acgtcgtccc ccgagagggt gtcctccatc
     1261 ttcttggcca gcttgctctt cttgaggtac tcgttgagga tccgggtcag ggagctcttg
     1321 aagccgatca ggtgggtgcc gccctcccgg gtgttgatcc cgttcacgaa ggcgaacatg
     1381 gtctcgttga aggtgtcgtt gtactggagg ccgacctcga tctccacgtc gtcccgggta
     1441 ccggagaaat agatgggctc cttgtggagg accgtcttgt tcttgttcag gtactccacg
     1501 aactgccgga gtcccccgtc gaagcggaac tcgtgggtct tgggcgtcga gagccgctcg
     1561 tccgtcaggg tgatcgtcac gcccttgttc aggaaggcca gctcccggag ccgtcccgcc
     1621 aggacgtcga aggagaagac cgtggactcg aagatcgagg ggtcgggctg gaagcggact
     1681 acggtccccc gcttctccgt ggttcccagg atggagacgg gggcctcggg aatccccatc
     1741 cggtagcgct ggaaatggat ggacccgttc aggtggacga aggcctcgca gacggaggac
     1801 agggcgttga ccaccgagac cccgacgccg tgcaggcctc cggatacctt gtagga
//
LOCUS       DAOTEI010000288         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_56666_length_1842_cov_5.106323, whole genome shotgun sequence.
ACCESSION   DAOTEI010000288 DAOTEI010000000
VERSION     DAOTEI010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56666_length_1842_cov_5.106323"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="P5313_16710"
     CDS             complement(<1..1210)
                     /locus_tag="P5313_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRY82059.1"
                     /translation="MNKAKRSLIVLVVLALAALFLLPSIRWYFFTPKDEKILATGSSE
                     QIREYAWRMARNDLAELEKAARAPGDEPLPAGIEWMADRAKKLYKELGKAAPSSWTAK
                     SVLAAYDSRKTAMSDIEDHYRTRILKIKNLQNTVVQLGLDLSGGMSVVIRADLPALEA
                     KLGHSLSLSEREEAMSRALEVLNNRIDKFGLTEPVIRRQGEDGIYVEIPGAADPERIN
                     SIIMGRGRLAFHIVDDEATARLDDYLTTNPAGIGADLKVADPSIVPAGYVVRKFYKKD
                     KYGLDEWTGRYLVITEQPGLDGNTIQRAERTNDPITGEPNIIFVLDNEGGEIFYALTS
                     ANVGKTLAVVLDDRIKAGARISEAIRESVRMTGFSVEESANLALILRSAALPVELVVE
                     SQQAIGASLGE"
     gene            complement(1277..1669)
                     /gene="yajC"
                     /locus_tag="P5313_16715"
     CDS             complement(1277..1669)
                     /gene="yajC"
                     /locus_tag="P5313_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRY82060.1"
                     /translation="MNTLLTSLPVLQAPAGTTGSMMSTIVMFGLIFVIFYFLIIRPQN
                     KKQKELQKMLAGIQKGDKVVTIGGVHGVVHAVKEGGTVVIKVDDTCKIEFSKSAVATI
                     LNRDSSKESSEKAPSEKSGADKGEEAAK"
BASE COUNT          297 a          569 c          616 g          360 t
ORIGIN      
        1 cctcccccag ggaggcgccg atggcctgct ggctttccac gacgagctcc acagggaggg
       61 ccgcggagcg caggatcagg gccaggttgg cggattcctc cacgctgaag cccgtcatgc
      121 ggacggactc ccggatggcc tcggagatgc gggcgcccgc cttgatacgg tcgtccagga
      181 ccaccgccag ggtcttgccc acgttggcgc tggtcagggc gtagaagatc tcaccgccct
      241 cgttgtccag cacgaagatg atgttgggct ccccggtgat cgggtcgttg gtccgctcgg
      301 cccgctggat ggtgttgccg tccaggccgg gctgctcggt gatcaccagg tagcggcccg
      361 tccactcgtc caggccgtac ttgtccttct tgtagaactt ccggaccacg tagcccgccg
      421 gcacgatgga gggatccgcc accttgaggt ccgcgccgat tcccgcggga ttggtggtca
      481 ggtagtcgtc cagccgcgcg gtggcctcgt cgtccacgat gtggaaggcc agccgacccc
      541 gccccatgat gatggagttg atccgctcgg ggtccgccgc gccggggatc tccacgtaga
      601 tcccgtcctc gccctgccgg cggatgaccg gctcggtcag gccgaacttg tcgatgcggt
      661 tgttcaggac ttccagggcc cgggacatgg cttcctcgcg ctccgagagg gagagcgaat
      721 ggccgagctt ggcctccagg gccggcaggt cggcgcgaat gaccacgctc atgccgccgg
      781 agaggtccag gcccagctgg accacggtgt tctgcaggtt cttgatcttg aggatccggg
      841 tccggtagtg atcctcgatg tcggacatcg cggtcttccg ggaatcgtag gccgccagga
      901 cgcttttcgc ggtccaggac gagggggcgg ccttgccgag ctccttgtac agcttcttgg
      961 cgcgatcggc catccactcg atgcccgccg gcaggggctc gtccccgggc gcccgggcgg
     1021 ccttctccag ctcggccagg tcgttccggg ccatgcgcca ggcgtattcc cggatctgtt
     1081 cgctggaacc cgtggccagg atcttctcgt ccttgggggt gaagaaatac caccggatcg
     1141 agggaagcag gaacagggcg gccagcgcca gcacgacgag cacgatgagt gagcgcttcg
     1201 ctttgttcat tcgaaaactc cagtcgtcac gaggatgggg tgccgcgggg ggcgccgcgg
     1261 aggcgccccg gcgggattac ttcgcggctt cctcgccctt gtcggcgccc gacttctcgg
     1321 agggggcctt ctcggaggat tccttggagg agtcgcggtt caggatcgtc gcgaccgcgg
     1381 atttggagaa ctcgatcttg caggtgtcgt ccaccttgat gaccacggtt ccgccttcct
     1441 tcacggcgtg gacgaccccg tggacgccgc cgatggtgac gaccttgtcg cccttctgga
     1501 tgcccgcgag catcttctgg agttccttct gcttcttgtt ctggggccgg atgatgagga
     1561 agtagaagat cacgaagatg aggccgaaca tcacgatggt ggacatcatg gatcccgtgg
     1621 ttccggccgg ggcctggagg acgggaaggg aggtaagcag ggtattcatg agcgctttcc
     1681 ttaaagagac agagttgtcg aaaggtacca cggaaggggg gcttggtcaa tccgtggaga
     1741 aagacggtga atgggtgaat agtgaatagg gggctgctta attcacccat tcacccattc
     1801 acccattcac ccattcaccc attcacccat tcacccattc ac
//
LOCUS       DAOTEI010000289         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_64828_length_1706_cov_4.077529, whole genome shotgun sequence.
ACCESSION   DAOTEI010000289 DAOTEI010000000
VERSION     DAOTEI010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64828_length_1706_cov_4.077529"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..>1706
                     /locus_tag="P5313_16720"
     CDS             157..>1706
                     /locus_tag="P5313_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82061.1"
                     /translation="MYFNELVAAYDQERRDRNIFHDLMRYRVREILLVASLYDSFILE
                     SDGALSEQIYGEYYRLNLTSAPRVTCAYSPESAQETFRSGPFDLVILMAGMDFDGPLA
                     LARDLKAVRPEVPVILLAMNNSSLADLDPKRPELRYADRVFVWNGYSKLFVGMIKYVE
                     DLRNVDSDTRTGLVRVVLLVEDSVRYYSRYLPVLYSVVMRQIQALIEEEQLVETYKVL
                     RMRGRPKILLATSYEEAVALFERYEPYILTVISDVRYSCGGTEDPRAGFKLLDHIRES
                     SPDLPVLIQSSEPEARTEAHRLGAGFISKGSDSLARELDGFFRENLGFGPFIFRGPDG
                     TELARARNMAEFEERLLQVPSDSLLLHGKRNHFSAWLTARGEIRFARILRNYRIDDFS
                     DPQEMRDFLLRLLDDVRRSKNRGLIPHFDVSLCGDSNVMARLAAGSVGGKGRGLMFLK
                     SLIDNMAFPAWEGKLEIRVPFTIFIGIDEFERFLESNNLWSFAYGGGDYEEIRRRYET
                     DPEACRLA"
BASE COUNT          289 a          583 c          528 g          306 t
ORIGIN      
        1 ggggggctcc gtggaccgag atttgcgtat gatgccgggt tgcgcgccat cccgctggga
       61 ttccgggatg ccgctgctac cagtatacga taaaccgccc gcgctgtcag ggtttcccct
      121 tgaagaaccg atcgccgccg gacatactat ctacacatgt acttcaacga gctcgtcgcg
      181 gcctacgacc aggaacgccg cgaccggaac atcttccacg acctcatgcg ataccgggtc
      241 cgggagatcc tgctggtggc cagcctgtac gattccttca tcctggagag cgacggggct
      301 ctctcggagc agatctacgg ggagtactac cgcctcaacc tgacctcggc cccccgggtc
      361 acctgcgcct acagccccga atccgcccag gagaccttcc gctccggccc cttcgacctg
      421 gtcatcctga tggcggggat ggatttcgac ggtcccctgg ccctggcccg ggacctgaag
      481 gccgtccggc cggaggtgcc cgtcatcctc ctggccatga acaactccag cctggcggac
      541 ctggacccca agcgtcccga gctccggtac gcggaccggg tcttcgtctg gaacgggtat
      601 tccaagctct tcgtggggat gatcaagtac gtggaggacc tgcggaacgt ggactccgac
      661 acccgcacgg gcttggtgcg ggtcgtcctc ctggtcgaag actcagtccg ttattattcc
      721 cggtacctgc cggtgctcta ttccgtcgtc atgcgccaga tccaggccct gatcgaggaa
      781 gagcagctgg tggagaccta caaggtcttg cgcatgcggg gccgccccaa gatcctcctg
      841 gccaccagct acgaggaggc ggtggccctc ttcgagcggt acgagcccta catcctgacc
      901 gtcatctccg acgtccggta ttcctgcggc gggaccgagg atccccgggc gggcttcaag
      961 ctcctggacc acatccggga gagcagcccg gaccttcccg tcctgatcca gtccagcgag
     1021 cccgaggccc gcacggaagc ccaccggctc ggggcgggct tcatatccaa gggatccgac
     1081 tccctggccc gggagctgga cggattcttc cgggagaacc tgggcttcgg acccttcatc
     1141 ttccgggggc ccgacgggac ggaactggct cgggcccgga acatggcgga gttcgaggaa
     1201 cgcctgctgc aagtgccctc ggacagcctg ctattgcacg gaaagcgcaa ccacttctcg
     1261 gcctggctca ccgcccgggg ggagatccgc ttcgcccgga tcctgaggaa ctaccggatc
     1321 gacgattttt cagatcccca ggagatgcgg gacttcctcc tgcgcctcct ggacgacgtc
     1381 cgccggtcca agaaccgggg gctcatcccg cacttcgacg tgtccctgtg cggggactcc
     1441 aacgtcatgg cccgcctggc cgcgggttcc gtgggcggca agggacgggg cctcatgttc
     1501 ctgaagagcc tgatcgacaa catggccttc ccggcctggg aagggaagct ggagatccgg
     1561 gttcccttca ccatcttcat cgggatcgac gagttcgagc gcttcctgga atccaacaac
     1621 ctctggtcct tcgcctacgg gggaggcgac tacgaggaga tccgccgccg ctacgagacc
     1681 gatcccgagg cctgccgcct ggccga
//
LOCUS       DAOTEI010000290         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_66502_length_1681_cov_2.704797, whole genome shotgun sequence.
ACCESSION   DAOTEI010000290 DAOTEI010000000
VERSION     DAOTEI010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66502_length_1681_cov_2.704797"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="P5313_16725"
     CDS             complement(<1..1002)
                     /locus_tag="P5313_16725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY82062.1"
                     /translation="MKSDPLVTVLMPVYNGGKYLRPTMETILGQTYRDFEFLLINDCS
                     TDDSLETIRSFSDPRIRIHTNEKNMGQTPSLNVGLKLARGKYIVVNDADDFSLPKRIE
                     TQLDFIQKNPEYPVVGCSAYIMDKDGVINRVFRKPTDPRKIRLWILTDTPMIHGAVIM
                     NKEIIRAEGGYDEYHITSQDSEFWSRLMRKGYRVTNVPDVLVVIRHYTASMSSRAADR
                     QMVEAGRIFRANINGMTSLNITDEEAIRHRTIFIAPEQLSEDEFLKAEELLVRAYGNL
                     KDGTGLEPEFIAEDLREKLVKPYVKRAADRLRQGRTAEARRIVRRYLATYGNNRLLVL
                     "
     gene            complement(1010..>1681)
                     /locus_tag="P5313_16730"
     CDS             complement(1010..>1681)
                     /locus_tag="P5313_16730"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRY82063.1"
                     /translation="IAADRHSEALIVEQLAASGIPLYSEEAGAIDTRRAARRSSKAAG
                     PLWIIDPLDGSLNYHQGIPLCCVSIALYEGPRPILGVVYDFNHDEMFCGLAGRGAWLN
                     HSPMEPSRTDFVSRAVLATGFPVRTDYSGPALSEFVGAVQGFRKVRLLGSAALSLCYV
                     ACGRIDAYREDAIMFWDVAAGCAIVEAAGGTVSVENLEHPASPVHVLATNGRLSADEI
                     RTSQG"
BASE COUNT          281 a          495 c          562 g          343 t
ORIGIN      
        1 cagaaccaat aggcggttgt tcccgtatgt ggccaggtat cgccggacaa tccttctggc
       61 ctccgctgtc cggccctggc gtaatcggtc tgccgcgcgc ttcacataag gtttcacgag
      121 tttttctcgc agatcctccg cgatgaattc aggctccagc cccgtgccgt ctttcaggtt
      181 cccgtaggcc cgaacaagaa gctcctcggc cttgaggaac tcgtcctcgc tgagctgttc
      241 cggcgcgatg aagattgtcc ggtgccggat ggcctcctcg tcggtgatgt tcagggaggt
      301 catcccgttg atgttggccc ggaatattct gcccgcctcg accatctgac gatccgcggc
      361 ccgggagctc atggaggcgg tgtagtggcg gatgacgacg aggacgtcgg ggacgttggt
      421 cacgcgatac cccttgcgca tcagcctcga ccagaactcg gagtcctggc tcgtgatgtg
      481 gtactcgtcg tagcccccct cggcgcggat gatctccttg ttcatgatca cggcgccgtg
      541 gatcatcggg gtgtccgtca ggatccagag ccggatcttt cgggggtccg tcggtttccg
      601 gaagacccgg ttgatcaccc cgtccttgtc catgatgtag gccgagcagc cgaccacggg
      661 gtattcgggg tttttctgga tgaaatcgag ctgcgtctcg atgcgcttcg gaagggagaa
      721 atcgtcggcg tcgtttacga cgatgtattt gccccgggcg agcttcaacc cgacattcag
      781 agaaggggtc tgccccatgt tcttttcgtt ggtgtggatg cggatcctcg ggtcgctgaa
      841 ggaccggatc gtctccagtg agtcgtcggt gctgcaatcg ttgatgagga ggaactcgaa
      901 gtcccgatac gtctggccca ggatggtctc catggtgggc cggaggtact tgcccccgtt
      961 gtagacgggc atcaggacgg tgaccagggg atctgatttc atgaaagggt tatccttgcg
     1021 aggttcggat ctcgtcggcc gaaagacggc cgttggtggc gaggacatgg accggcgagg
     1081 cggggtgctc gagattctcc accgataccg tgccgccggc cgcttcgacg atggcgcagc
     1141 ccgccgccac gtcccagaac atgatggcgt cttcccggta ggcgtcgatg cggccgcagg
     1201 ccacatagca caaagacagc gccgcgctgc ccagcaggcg gaccttgcgg aatccctgca
     1261 cggccccgac gaattccgag agggccgggc ccgagtaatc ggtgcggacg gggaatcccg
     1321 tggcgagcac cgcccgggaa acgaagtccg tccgggaagg ctccatggga ctatggttca
     1381 gccaggcccc gcggccggca agtccgcaga acatctcgtc gtggttgaag tcatagacga
     1441 cgccgaggat gggccggggg ccctcataga gggcaatgga tacgcagcac agggggattc
     1501 cctggtgata gttcaggctc ccgtcgaggg ggtcgatgat ccacaggggg cctgccgctt
     1561 ttgaggagcg tcgcgctgcc cgcctggtgt cgatcgcccc ggcttcttcg ctgtacaggg
     1621 ggatgccgct tgccgccagc tgctccacga tgagggcttc cgagtggcgg tcggcggcga
     1681 t
//
LOCUS       DAOTEI010000291         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_66820_length_1676_cov_4.736582, whole genome shotgun sequence.
ACCESSION   DAOTEI010000291 DAOTEI010000000
VERSION     DAOTEI010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66820_length_1676_cov_4.736582"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..335
                     /locus_tag="P5313_16735"
     tRNA            262..335
                     /locus_tag="P5313_16735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Arg,seq:ccg)
     gene            380..982
                     /locus_tag="P5313_16740"
     CDS             380..982
                     /locus_tag="P5313_16740"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRY82064.1"
                     /translation="MDTILLASGSPRRREILEQYGFPLVVRPTDTDETLRDHLPVGDR
                     VTALALDKARAALADSRDGDPRWVLSADTLVSVDGRVLGKPRNRDEARTFLRSLSGVP
                     HRVSTGLAFTDRNSGAVRTILEETEVVFAELSDDEIEGYLDTGEWEGAAGAYRIQGRA
                     AFLVERVIGSWSCVVGLPLRAFYVILRDSGYPLGDRPERP"
     gene            1085..>1676
                     /gene="rpsB"
                     /locus_tag="P5313_16745"
     CDS             1085..>1676
                     /gene="rpsB"
                     /locus_tag="P5313_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRY82065.1"
                     /translation="MAVVTMKNLLESGVHFGHQVKRWDPRMKKFIFAERNGIHIIDLQ
                     KTIQSIKEAYEAVRKCVASGKSVLFVGTKKQAQQAIQKEAERCGMFYVNSRWLGGMLT
                     NFSTIKKSILRLKKIEKMEVDGTFENLTKKEIAGLQKEKIKLEKNLGGIKEMKDLPGI
                     LFVIDTRKEAIAVAEAQRLGVPIVAIVDTNCNPEGIT"
BASE COUNT          325 a          515 c          540 g          296 t
ORIGIN      
        1 cccggtcaag cagaaggcgc gccaggtgct ggacaagctc cgcaagtccg cggcgagctc
       61 ctcgggcggc aaatgacgca gtattccatg aggccggctc ctaagctctg ttagcttcac
      121 ggccggccgg ttccggagcg gcggttcgca agggtttcgc ggggaaggtt tcgatccgcg
      181 aggggggtcc ggcggacgac ggctgggtgg actccccggg ttcccttgac aggctcccgg
      241 cttccgacat aggatgtccc cgagcgatta gctcagctgg atagagcgtc ggcctccgga
      301 gccgaaggtc atgggttcga atcccgtatc gctcataggc ggctttcagg agcaaggctc
      361 cggaaagccg ttttttatta tggatacaat tcttctagcc tcgggatccc cgcgtcgaag
      421 ggagatcctt gaacagtacg gatttcccct ggtcgtgcgg cccaccgata cggacgaaac
      481 cctccgggat catcttccgg tgggcgatcg cgtcacggcc ctggccctgg acaaggcccg
      541 cgccgccctg gccgactccc gggacggcga tccccgatgg gttctgtccg cggacaccct
      601 ggtttccgtg gacggaaggg tcctgggaaa gcctcgaaac cgggacgaag cccgcacctt
      661 tctccgctcc ctgtccggcg tcccccatcg ggtctccacg ggcctcgcct tcaccgaccg
      721 gaactccgga gccgtccgga cgatcctgga ggaaacggag gtggtcttcg cggaactctc
      781 ggacgacgag atcgaggggt acctggatac gggggaatgg gaaggcgccg ccggggccta
      841 tcgcatccag ggccgggcgg ccttcctcgt ggagcgtgtc atcggatcct ggtcctgcgt
      901 cgtgggcttg cctctccggg cgttttatgt catccttcgg gacagcggtt accccctggg
      961 tgaccgcccg gagcggccct aaccctgggt tttagccgcc cggaatcttc cgccgtcccc
     1021 ccgggacggt gagaatcaga gaagggggcc gtacgaaccg tacggccgtc ggtaggagga
     1081 tctcttggcc gtcgtaacga tgaagaatct tctggagtcc ggtgtccact tcgggcatca
     1141 ggtcaagcgc tgggatccgc ggatgaagaa attcatcttc gcggagcgca acggcatcca
     1201 catcatcgat ctgcagaaga ccatccagtc catcaaggaa gcctatgagg ccgtgcggaa
     1261 gtgcgtagcc tccggcaaga gcgtgctctt cgtgggcacc aagaagcagg cccagcaggc
     1321 catccagaag gaagccgagc gctgcgggat gttctacgtg aacagccgct ggctgggcgg
     1381 aatgctcacc aacttctcca ccatcaagaa atccatcctc cggctcaaga agatcgagaa
     1441 gatggaagtg gacggcacct tcgagaacct gaccaagaag gaaatcgccg ggctccagaa
     1501 ggagaagatc aagctggaga agaacctggg cggtatcaag gagatgaagg atctgcccgg
     1561 catcctcttc gtgatcgaca cccgcaagga agccatcgcg gtggcggaag cccagaggct
     1621 gggcgtgccc atcgtggcca tcgtcgacac caactgcaat cccgagggca tcacct
//
LOCUS       DAOTEI010000292         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_69015_length_1645_cov_5.325786, whole genome shotgun sequence.
ACCESSION   DAOTEI010000292 DAOTEI010000000
VERSION     DAOTEI010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69015_length_1645_cov_5.325786"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="P5313_16750"
     CDS             complement(<1..1114)
                     /locus_tag="P5313_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRY82066.1"
                     /translation="MRDIQDFDSIMIQLASPDMIRAWSYGEVKKPETINYRTLRPEKD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVICDRCGVEVTHFKVRRERMGHIELASPVS
                     HIWFYRSVPSRMGLLLDIPVAALRSVLYYEKFIVIDPGDTDLKKMQLLTEEEYYEAQD
                     RYGGAFSAGMGAEAIRSLLEQIDLEQMAAELRAKMIEKGAKSDKRLLKRIEIVESFRT
                     SQNRPEWMILDVIPVIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLQSLNA
                     PDIIIRNEKRMLQEAVDALFDNSKKKRVVKGASNRPLKSISDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKMHQCGLPTKMALELFKPFIM"
     gene            complement(1115..1261)
                     /locus_tag="P5313_16755"
     CDS             complement(1115..1261)
                     /locus_tag="P5313_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82067.1"
                     /translation="MEWKKVREIDLGGFSPETATPLSFLFSVYSVSSVVDSDNSPICM
                     AEGI"
     gene            complement(1312..>1645)
                     /locus_tag="P5313_16760"
     CDS             complement(1312..>1645)
                     /locus_tag="P5313_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82068.1"
                     /translation="VTQQPLGGKAQFGGQRLGEMEVWALEAYGAANTLQELLTIKSDD
                     MTGRAKVYEAIVKGEPHAAAGIPEAFNVMVQELRGLALDIRVYDAKGKQIALTERDED
                     LINKQGTF"
BASE COUNT          261 a          509 c          536 g          339 t
ORIGIN      
        1 tcatgatgaa gggcttgaag agctccaggg ccatcttggt cgggaggccg cactggtgca
       61 tcttgagctc cgggccgacc acgatcaccg agcggccgga atagtccacg cgcttgccga
      121 ggaggttctg gcggaaccgg ccctgcttgc ccttgagcat gtccgagatg gacttcaggg
      181 ggcgattaga ggctcccttg accacccgct tcttcttgga gttgtcgaag agggcgtcca
      241 ccgcctcctg gagcatccgc ttctcgttcc ggatgatgat gtccggggcg ttcagggact
      301 ggaggcgctt gaggcggttg ttccggttga tcacccggcg gtacaggtcg ttcaggtcgg
      361 aggtggcgaa gcgtccgccg tccagctgga ccatggggcg gatctccggg gggatcacgg
      421 ggatcacgtc caggatcatc cactccggcc ggttctggga ggtccggaag gactcgacga
      481 tctcgatgcg cttcagcagg cgcttgtcgc tcttggcgcc cttctcgatc atcttggccc
      541 ggagctccgc ggccatctgc tccaggtcga tctgctccag gagggagcgg atggcctcgg
      601 cgcccatgcc cgcggagaag gccccgccgt agcggtcctg ggcctcgtag tactcctcct
      661 ccgtgaggag ctgcatcttc ttgaggtccg tgtcgcccgg atcgatgacg atgaactttt
      721 cgtagtagag gaccgagcgg agggccgcca cggggatgtc caggaggagc cccatgcggc
      781 ttggcacgga gcggtagaac cagatgtggg acaccgggga ggccagctcg atgtggccca
      841 tgcgctcccg gcggaccttg aagtgggtca cctcgacgcc gcagcggtcg cagatcacgc
      901 ccttgtagcg gatgctcttg aacttgccgc agtagcactc ccattccttg gtggtgccga
      961 agatccgctc gcagaagagg ccgtccttct cgggcctcag ggtgcggtag ttgatggtct
     1021 cgggcttctt gacctcgccg tagctccacg cccggatcat gtccggcgag gccaactgga
     1081 tcattatgga atcaaaatcc tggatatccc gcatctaaat cccctccgcc atacaaatgg
     1141 ggctgttgtc ggaatcaacc acggaggaca cggagtacac ggagaagaga aatgaaaggg
     1201 gtgtagcggt ctcggggctg aagcctccga ggtctatctc cctcactttc ttccactcca
     1261 tcttatctcc gtgccctccg tgatctccgt ggtgaattcc tgcgaagtcc tctagaacgt
     1321 tccctgcttg ttgatcaggt cctcgtcgcg ctcggtcagg gcgatctgct tgcccttggc
     1381 gtcgtagacc cggatgtcca gggccaggcc ccggagctcc tggaccatga cgttgaaggc
     1441 ctcgggaatg cccgccgccg cgtggggttc gcccttcacg atggcctcgt agaccttggc
     1501 ccggccggtc atgtcgtcgc tcttgatcgt caggagctcc tggagggtgt tggcggcgcc
     1561 gtaggcctcc agggcccaga cctccatctc tcccaggcgc tggccgccga actgggcctt
     1621 gccgcccagg ggctgctggg tgacc
//
LOCUS       DAOTEI010000293         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_69272_length_1642_cov_2.401386, whole genome shotgun sequence.
ACCESSION   DAOTEI010000293 DAOTEI010000000
VERSION     DAOTEI010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69272_length_1642_cov_2.401386"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1057
                     /locus_tag="P5313_16765"
     CDS             158..1057
                     /locus_tag="P5313_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82069.1"
                     /translation="MRTPGGPGARPGRVRAVLAALACATAAPSLAQPVLLAYAGTDSY
                     VVTEKSDWSRYEDGRYLGHVYREVRGRLEPESPSPDALWKGDFLVFEETLRDLSAAAR
                     RVDDQVPAAFRMGTDGSLRMTVDNGFPGLRGFPAFPPGPVSPGDRWTAEGVRVVDPRN
                     SGVPTRTRILAEYEYRGEETYKGIPVRRIFAKFAVRYRAGQDPKGDPSLVEAQGTHEA
                     DILVRSDTGRPVLIRDRLDETFRFASGPSLRHRGFTLTFFDAAIPRTADPASGALRAA
                     LAEEAPGAAAVPGPSSSSAQLNT"
     assembly_gap    1077..1086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1239..>1642
                     /locus_tag="P5313_16770"
     CDS             1239..>1642
                     /locus_tag="P5313_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY82070.1"
                     /translation="MGAGTEGPLSTGSSSLDPGSGIALEDRPEGPTLVAFGLRFVPDQ
                     DVLLPEEKGRLDGIAAALKSVPGERTLLVTGHTADVGNPAGQKDLSIRRAKRVVDELV
                     ARGIPASRFLFRGLGAERPIAPYDTEACCARNR"
BASE COUNT          208 a          623 c          571 g          230 t
ORIGIN      
        1 ttcaaacaga cggtgaaaaa ttctgccgcg ggccgccgag gccgcgccat caagattgag
       61 ggtgtgcctc aagtttacaa gcgccccgcc ttcaagctca gagggcgcct gcctgataaa
      121 gctccgccgt ctgcggcctc ccgggccggg acgccgcgtg aggaccccgg gcggccccgg
      181 cgcgcgcccc gggagggtcc gggccgtact ggccgccctc gcctgcgcga cggcggcccc
      241 gagtctcgcc cagcccgtcc tcctggccta cgccgggacc gattcctacg tggtcaccga
      301 gaagtccgac tggtcccgct acgaggacgg ccgctaccta gggcacgtct accgggaggt
      361 ccggggtcgt ctggagcccg agagcccctc cccggacgcc ctctggaagg gggacttcct
      421 ggtcttcgag gagaccctac gggacctgag cgccgcggcc cgacgggtgg acgaccaggt
      481 ccccgcggcc ttccgcatgg ggacggacgg gtccctacgc atgaccgtgg acaacgggtt
      541 tcccggactc cggggcttcc ccgccttccc gcccggtccc gtctcccccg gggaccgctg
      601 gaccgcggag ggggtccggg tcgtggatcc ccggaactcc ggcgtgccca cgcgcacccg
      661 gatcctggcg gagtacgagt accgggggga ggagacctac aagggcatcc cggtacggcg
      721 catcttcgcc aagttcgcgg tccggtaccg ggcgggccag gatcccaagg gggacccgtc
      781 cctcgtggag gcccagggga cccacgaggc ggatatcctg gtgcgctcgg acacgggccg
      841 ccccgtgctc atccgggacc gcctggacga gaccttccgg ttcgcctcgg ggccctccct
      901 ccggcaccgg ggcttcaccc tgaccttctt cgacgccgcc atcccgagga ccgcggaccc
      961 cgcgtcggga gccctgcgag cggctttggc cgaggaagct ccgggggcgg ccgccgtgcc
     1021 cggcccgtcc tcctcgtcgg cgcaattgaa cacgtaggta actgaggcgt gccagtnnnn
     1081 nnnnnncgtc ggcgccgcct ccgaaacccg cggtccccgg cccgggtgcc ggatcttcga
     1141 gcccgtcggc cgccccgatc gtctctccgg ccccgggcac ggcgggtatg ccggccggat
     1201 ccccgcctgg cgcttcggcc cctcccggct ccggtcttat gggcgccggc accgagggtc
     1261 cgctctcgac ggggtcctcc tccctggacc caggatccgg gatcgccctg gaggaccggc
     1321 ccgaggggcc gaccctggtg gccttcgggc tccgtttcgt cccggaccag gacgtcctcc
     1381 tgcccgagga gaagggccgc ctggacggga tcgccgcggc cctgaagtcc gtgcccgggg
     1441 aacgcaccct tctggtgacc ggccacaccg cggacgtggg caatcccgcg ggccagaagg
     1501 acctgtcgat ccgccgggcc aaacgggttg tggacgagct cgtcgcccgg ggcatcccgg
     1561 cctcccggtt cctcttccgg ggcctcgggg cggagcgtcc gatcgcgccg tacgacacgg
     1621 aggcgtgctg cgcccgcaac cg
//
LOCUS       DAOTEI010000294         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_71114_length_1617_cov_4.114597, whole genome shotgun sequence.
ACCESSION   DAOTEI010000294 DAOTEI010000000
VERSION     DAOTEI010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71114_length_1617_cov_4.114597"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="P5313_16775"
     CDS             complement(<1..519)
                     /locus_tag="P5313_16775"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82071.1"
                     /translation="MYRCEIRGFPVPVVTLHPGEYYSTGEDLVLSTVLGSCVAVALFD
                     VETRIGGLNHFMLPGSVDTAKPLLSESARYGMYAMELLINDMLKKGVRKRDLRAKVFG
                     AASLLEFSGGSAGRISDSNIRFTFSYLEGEGIPVLARDVGGDLPRKIFFYARTGRVLL
                     KRIPRTRLTAIRR"
     gene            complement(526..>1617)
                     /locus_tag="P5313_16780"
     CDS             complement(526..>1617)
                     /locus_tag="P5313_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82072.1"
                     /translation="EEAAAAFGTLSQEAAGVRNPILGIMQGVQVQDILRQSIDHVILS
                     LREVFKDPTVSLKDELVFTAAVADLSVSVLADVIERIREGVSGMGGHVDEVVKFVEDL
                     EVRRQRTVSICEDFGIGSGEAAGKAETYLALKGRALAAGRSLSEQVRQLNDSFRVLAS
                     LLGKFQNIVVASRIEIARTRALSVVSNTVAGMIEITESLGRDVGQATDLTKTFIKTAS
                     AEILAYDKVQERNDERLALSVRRMEDELRRLESSRERVREAIASFRLYTPQFLDLIRT
                     ARASLDRLARLKDTLELLKKEIETLKRRASLEAEGIEGGGDDLHSIRNDRLRRMVERF
                     TIFTHKKTAGVLGDFAVEEGVESGEVTLF"
BASE COUNT          262 a          559 c          533 g          263 t
ORIGIN      
        1 cctccggatc gcggtgagcc gggtccgggg gatccgcttg agcaggaccc ggccggtccg
       61 ggcgtagaag aaaatcttcc ggggcaggtc ccctcccacg tcccgggcca ggacggggat
      121 gccctccccc tccaggtagg agaaggtgaa ccggatgttg ctgtccgaga tccggcccgc
      181 ggagcctccc gagaattcca gcagggaggc ggccccgaag accttggccc ggaggtcccg
      241 cttccggaca cccttcttca gcatgtcgtt gatcaggagc tccatggcgt acatcccgta
      301 ccgggcgctc tcggaaagca ggggtttggc ggtgtccacg gaccccggga gcatgaagtg
      361 gttcaggccc ccgatccgcg tctcgacgtc gaaaagggcc accgccacac aggagcccag
      421 gaccgtggag agcacgaggt cctcgccggt ggagtagtac tcgccggggt gcagggtgac
      481 gaccggaacc gggaatcccc ggatctcgca gcggtacacg gtccctcaga acagggtgac
      541 ttcgccggac tccacccctt cctccacggc gaagtccccc aggacccccg cggtcttctt
      601 gtgggtgaag atcgtgaagc gctcgaccat gcggcgcagg cggtcgttcc ggatggagtg
      661 cagatcgtcc cctcccccct cgatcccctc ggcctccagg gaggcccgcc gcttgagggt
      721 ctctatctcc tttttaagga gttccagggt gtccttgagc cgggcgagcc ggtccaggga
      781 ggcccgggcc gtccgtatga ggtccaggaa ctgcggagtg tagagccgga acgaggcgat
      841 cgcctcccgg acccgttccc gggaggactc caggcgccgg agctcgtcct ccatgcggcg
      901 cacggaaagg gccagcctct cgtcgttccg ctcctggacc ttgtcgtagg ccaggatctc
      961 cgcgctggcg gtcttgatga aggtcttggt caggtccgtc gcctggccca cgtcccgccc
     1021 caggctctcg gtgatctcga tcatcccggc cacggtgttg gaaaccacgg acagggcccg
     1081 ggtacgggct atctcgatcc gggacgcgac gacgatgttc tggaacttgc ccagcaggga
     1141 cgcgaggacc cggaaggagt cgttcagctg ccggacctgc tcggaaaggc tccggcccgc
     1201 cgccagggcc cgccccttca gggccaggta ggtctccgcc ttccccgcgg cctccccgga
     1261 gccgatcccg aagtcctcgc agatgctcac ggtccgctgg cgccggacct ccaagtcttc
     1321 cacgaacttg acgacctcgt cgacgtggcc ccccatgccg gacacgcctt cccggatgcg
     1381 ctcgatcacg tccgcgagca ccgacaccga aagatccgcg accgcggcgg taaagaccag
     1441 ttcgtccttg agggagacgg tcggatcctt gaacacctcc cggagggaga gtatgacgtg
     1501 gtcgatggac tggcggagga tgtcctggac ctggaccccc tgcatgatcc ccaggatggg
     1561 attccggacc cccgcggcct cctgggacag ggttccgaag gcggcggccg cctcctc
//
LOCUS       DAOTEI010000295         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_74343_length_1575_cov_5.015789, whole genome shotgun sequence.
ACCESSION   DAOTEI010000295 DAOTEI010000000
VERSION     DAOTEI010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74343_length_1575_cov_5.015789"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="P5313_16785"
     CDS             complement(<1..297)
                     /locus_tag="P5313_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRY82073.1"
                     /translation="MARDDSDNERFLRSLGFPSLRVHDSFNHFDFTEPGRRVLVIGPM
                     GSGKTEFSARIWRDSRVALKKGGSVAAATSSQGADRRKLFFVRSSLDRSRFPDYP"
     gene            complement(368..1384)
                     /locus_tag="P5313_16790"
     CDS             complement(368..1384)
                     /locus_tag="P5313_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82074.1"
                     /translation="MRKLLALALYVFIGLPATLGTLFLVPIRGWVLDRDFYKAVVSGP
                     EARAVLEAPALYDRFEETIRAGDSLTLSGPATGKALKASLPVPELLAAAGSAVDSAFD
                     ALERGGDANRFTVDLVPLRTALAKGVPEFARVYAAEVPVAASGAPAVVPARPEPGVPL
                     DLSVRPASVPESEFRAAIRSALAEAVAAIPDSAQPPMPEPGPGNAFLADARSTRDALN
                     KAALWMSLLAGGIWVAGAFVASDSAAKRLRWLGGTLLAPGIVVLTAGAVLRLAADPLV
                     LRVIQDPELQRALADPALAVVRDWIRRPLWTVSTGFLVSGSVAVILGGGLSASRRALR
                     YRDF"
BASE COUNT          257 a          529 c          595 g          194 t
ORIGIN      
        1 ggggtagtcc gggaagcgcg aacggtccag gctggagcgg acgaagaaga gcttccggcg
       61 atccgccccc tgggaggagg tggccgcggc caccgaaccg cccttcttca gggccacccg
      121 ggaatcccgc cagatccggg cggaaaactc ggtctttccg gagcccatgg gcccgatcac
      181 caggacacga cggccgggct ccgtgaagtc gaagtgattg aaggagtcgt ggacacggag
      241 ggaggggaat cccaggctcc gcaggaagcg ttcgttgtca gaatcgtcgc gcgccatcgt
      301 cgttcgctcc gtaccgtggg tgatccgggg acccggattc cgggcctccg tccggctcat
      361 tccgccgcta gaagtcccgg taccggaggg cccggcggga ggccgagagg cctccgccca
      421 ggatgaccgc cacggatccg gacaccagga accccgtgga cacggtccac aggggacgcc
      481 ggatccagtc ccgcaccacc gcgagggcgg ggtccgcgag ggcgcgctgg agctcggggt
      541 cctggatgac ccggagcacc agggggtccg cggccaagcg gaggactgcg ccggcggtca
      601 ggaccacgat ccccggggca aggagggtgc cccccagcca gcggaggcgc ttggccgcgg
      661 aatcggaggc cacgaaggcc ccggccaccc agatgccccc ggccaggagg gacatccaga
      721 gggcggcctt gttgagggcg tcccgggtgc tccgggcgtc cgccaggaag gcgttcccgg
      781 ggccgggctc cggcatcggg ggttgcgccg agtccgggat ggccgcgacc gcctcggcca
      841 gggccgatcg gatcgcagcc cggaactccg attccggaac ggaggccgga cgcacggata
      901 ggtccagggg aacccccggc tcgggccggg cggggacgac cgccggggct cccgacgccg
      961 ccacgggcac ctccgcggcg tagacccggg cgaactccgg cacacccttg gccagcgcgg
     1021 tccgcagggg taccaggtcc acggtgaagc ggttcgcgtc cccgccccgt tccagcgcgt
     1081 cgaaggcgga atccaccgcg gacccggcgg cggccaggag ttccgggacc gggagggagg
     1141 ccttcagggc ctttccggtc gcgggacccg agagggtcag ggagtccccg gcccggatcg
     1201 tttcctcgaa gcggtcgtac agggccgggg cttccaggac cgcccgggct tccggcccgg
     1261 acacgacggc cttgtagaaa tcccggtcca ggacccagcc ccggatgggc accaggaaga
     1321 gggtccccag ggtggcgggg aggccgatga agacgtagag ggccagggcg agcagtttcc
     1381 gcatgagggg ctccttgcta gtccgcgagt acgaggttgg gcagggcctt tggggagccc
     1441 acgcgcatgt acttgaaggg gtgcatgtcc agggcgttcg agtaccagcc ctccacgata
     1501 tcggtgtcca cgacgttcac cgccaggctg tggtagatcg gcaggaccgt gccggagtcc
     1561 aacaggactc cctcc
//
LOCUS       DAOTEI010000296         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_99714_length_1331_cov_4.006270, whole genome shotgun sequence.
ACCESSION   DAOTEI010000296 DAOTEI010000000
VERSION     DAOTEI010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99714_length_1331_cov_4.006270"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1331)
                     /locus_tag="P5313_16795"
     CDS             complement(<1..>1331)
                     /locus_tag="P5313_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82075.1"
                     /translation="AAADGAAALTKELGITAHILGRDPDTEDSTARLAVGSVEALSSR
                     QEAGSLDLGGFGFVVVDGLSRFIEPPFSGALRRIGARLRPPRERRTILFSERLGLKEQ
                     TLALELADAPEELYLEEEAEKTKHLPQTTWYVAAENKVRLLLGVLGSEPKGPVAVLCN
                     LPETAQDLVRRLGANGKRGEAVLENPAPSRKEAILARARSGELEAVVLTDESLRGLPR
                     GAFRQVINYDIPLEGEPYLERIRLLDASAPGARILNFACDRYVYGIPAVEQFMGISLG
                     AVQADDSLLAPADRSSAAAPGRGNRPRDDRNGWRSDRPQERGREDRPFRDGSPDRGQV
                     RREAGPRPRNEGGFRRDGRGRDREGDLRNQESIRAGIAELTGVNLGGSSARSRGPDAP
                     PPRDRESQDRGPRPEGPGRGNRRNKRGRRGPAGGGAPRGAGPVTQPSFADP"
BASE COUNT          161 a          484 c          491 g          195 t
ORIGIN      
        1 aggggtccgc gaaggagggc tgggtcacgg gtccggcgcc gcggggagcg cctccgcccg
       61 cgggaccgcg ccgtccgcgc ttgttcctcc ggttcccgcg gcccggcccc tcgggacggg
      121 ggccgcggtc ctgggattcc cggtcccggg ggggcggagc atccggacct cgggaccggg
      181 cgctcgatcc gccaaggttc acccccgtga gctccgcgat ccccgcccgg atggactcct
      241 ggttgcgcag gtcgccctcc cggtcgcggc cgcggccgtc ccggcggaag cccccttcgt
      301 tccgggggcg gggtcccgct tcccggcgga cctgtccgcg gtccgggctg ccgtcccgga
      361 aaggccggtc ctcccggccc cgttcctggg ggcggtccga cctccagccg ttccggtcgt
      421 cccggggacg attgccgcgg cccggggccg cggccgagct ccggtccgcc ggagccagaa
      481 gggaatcgtc ggcctggacg gcccccaggg agatgcccat gaactgctcc accgcgggga
      541 tgccgtagac gtagcggtcg caggcgaagt tcaggatccg agcccccgga gcggaggcgt
      601 ccaacagccg gatccgttcc aggtaaggtt ccccctccag ggggatgtcg tagttgatca
      661 cctggcggaa ggcgccccgg gggaggcccc ggaggctttc atccgtgagg accaccgcct
      721 ccaactcccc ggaccgggct cgggctagga tggcctcctt ccgggacggg gcgggattct
      781 cgaggacggc ttccccccgc tttccgttcg cgccgagtct gcggaccagg tcctgggcgg
      841 tctccggcag gttgcacagg acggcgacgg gacccttggg ctcggaaccc aggaccccta
      901 ggaggaggcg gactttgttc tccgccgcta cgtaccaggt cgtctggggg aggtgcttgg
      961 tcttctccgc ctcctcctcc aggtatagct cctcgggagc gtccgccagc tccagggcca
     1021 gggtctgctc cttcaggccc aggcgctcgg agaagaggat ggtccgccgc tcccggggag
     1081 ggcggagccg ggcgccgatc ctgcgaaggg ctccgctgaa ggggggctcg atgaatcggg
     1141 atagcccgtc cacgaccacg aagccgaagc ctcccaggtc caggctgccc gcctcctggc
     1201 gggaggacag ggcctccacg gaaccgacgg ccaggcgggc cgtggaatcc tcggtatccg
     1261 gatcccggcc caggatgtgc gcggtgatcc ccagctcctt cgtcagggcg gccgcgccgt
     1321 cggctgccgc g
//
LOCUS       DAOTEI010000297         1172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetia bacterium isolate AMR_MDS_3133
            NODE_125225_length_1172_cov_2.876455, whole genome shotgun
            sequence.
ACCESSION   DAOTEI010000297 DAOTEI010000000
VERSION     DAOTEI010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868157
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetia bacterium (wastewater metagenome)
  ORGANISM  Spirochaetia bacterium
            Bacteria; Spirochaetota; Spirochaetia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:36:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,360
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,307
            CDSs (with protein)               :: 3,307
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Spirochaetia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125225_length_1172_cov_2.876455"
                     /isolate="AMR_MDS_3133"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053615"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="P5313_16800"
     CDS             <1..1123
                     /locus_tag="P5313_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY82076.1"
                     /translation="RVAPTVGILSLDLERRPLARIFRSTGTAILFLFLLAAALALIVL
                     LVAYIRGLHRRAEQPVVAAVLDSEAAAAEERRRALASAPREKPAFVTRAEARAYASDL
                     VPKHVSSEEARRIQEGIVPKHLTAEEARVFQEETAPKHVTREEARIFEDSLRAQHVTE
                     EEARVFKPARKDITQDEARAYAAGRSLTEEEASRYRDTSARDAADALAAYAAASSPNR
                     PAPAASTGRPAEFTPVVKRTGSLRVEFRVEDQNPNIGLRNVRTLQSGGKKTIGGKRSD
                     FLVFLVPVPPRAAEVYFDGEDLTVVPLRPELFPDYTGPIPGCLGTPIRMVNRFGKVLT
                     LTFDRYVPPADRLNKILRCLESPGIPALNGDDTAGSGTA"
BASE COUNT          190 a          441 c          371 g          170 t
ORIGIN      
        1 ccgggtcgcg ccgaccgtcg gaatcctctc cctggacctc gaacgcagac ccctcgcccg
       61 gatcttccgt tcgaccggca cggccatcct cttcctgttc ctcttggcgg ccgcccttgc
      121 cctcatcgtc cttttagtgg cctacatccg cggcctccac cggcgcgccg aacagcccgt
      181 cgtggccgcg gtcctcgatt ccgaggccgc agccgcggag gagcgccgca gggccctggc
      241 ctcggccccc cgggagaagc ccgccttcgt cacccgggcg gaagcccggg cctacgcctc
      301 cgacctggtg cccaagcacg tgagctccga ggaggcccgc cgcatccagg aagggatcgt
      361 ccccaagcac cttaccgccg aggaagcccg ggtgttccag gaagagaccg ctccgaagca
      421 cgtcacccgg gaggaggctc ggatcttcga ggactctctc cgggcccagc acgtcacgga
      481 ggaggaggcc cgggtcttca agcccgcccg gaaggacatc acccaggacg aggcgcgggc
      541 ctacgcagcg ggccggtctc tcacggagga ggaagcctcc cgataccggg acacatcggc
      601 ccgggacgcc gccgacgccc tggccgccta cgcggccgcg tcctccccga accggccggc
      661 accggcggcc tccacgggcc gtcccgccga gttcaccccc gtcgtgaagc gcacgggctc
      721 tctcagagta gaattccggg tggaggacca gaatcccaac atcggcctcc ggaacgtccg
      781 gaccctgcag tccggcggca agaaaaccat cgggggcaag cgcagcgact tcctggtctt
      841 cctggtcccc gttccccccc gggccgcgga ggtgtacttc gacggcgagg acctgaccgt
      901 cgtacccctg cgccccgaac tcttcccgga ctacacggga cccatcccgg gctgcctggg
      961 cactccgata cggatggtca accggttcgg gaaggtgctg accctaacct tcgaccggta
     1021 cgtgccgccc gcggacaggc tcaacaagat cctgcgctgt ctggagtcgc cggggatacc
     1081 ggccttgaac ggggacgata cggccgggag cggtacggcc taggagcctg tcggacttgt
     1141 ggatttttag cgccgatagg cgctaaaaat cc
//
